LOCUS       DBNHKE010000001         6209 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_106083, whole genome shotgun sequence.
ACCESSION   DBNHKE010000001 DBNHKE010000000
VERSION     DBNHKE010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_106083"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="AC16O9_00005"
     CDS             complement(<1..1790)
                     /locus_tag="AC16O9_00005"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR6515034.1"
                     /translation="MSVPHPLAPELRAVAPRDLAGLLASLVLVAAPHALRMPWWLTLL
                     VLGLYAWRARIALERSALPSRWLVLALALLAVGAVYLEYRTLFGRTSGIVLLVLFSGL
                     KLAEMRTHRDAAVVAFLCYFLVMTNLLYTQSIPTALLMALALLAVTGTLVGLAAPQRP
                     ALANLRSAGVLLAHAAPAALILFLLFPRVQGPLWGLPQDAYAAMTGLSDTMTPGAVAQ
                     LAQSDAIAFRAEFEGEPPPPRERYWRGPVLWDFDGRTWRMGAGWLQKFDALQGGGRYE
                     YTVTLEPHNRTWMFALESVTRLPVRARYTNDGMVVATSPVRTRLRYDAASLARPEPEP
                     FERPDSLRRALSLPVSGNPRARALAAEWQRGARSDLEVLQRAVEFFRDSRLGYTLEPP
                     LLGRDTVDEFLFDTREGFCEHFSSAFVFLMRAAGVPARVVTGYQGGEVNYVDRIISVR
                     QSDAHAWAEVHLAGRGWVRADPTAAAVPGRIDAGLARAVRAGDPVPLLMRPQLEWLRG
                     LRSNWEALAHRWNVWVLGYGAERQRDLMSRIGMRDADWRKLTAAMASILGAFTLVLLV
                     WSLRRLARPDPVQRAWQAFCGKLRARGVARA"
     gene            complement(1787..2752)
                     /locus_tag="AC16O9_00010"
     CDS             complement(1787..2752)
                     /locus_tag="AC16O9_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6515035.1"
                     /translation="MIRALLNDARLRDWFYGYRAPERGAITLGQRRVYIVPTRLGWFY
                     GATLGILLVGSINYALQLGFALTFLLAGLGLVGMVHTTRNLARLTVSAGRAAPVFAGE
                     AAQFRLYLDNRARYDRPAILVRHQASGAQVVLDLAPGAVGEAVLPVPAARRGWLPLTR
                     VMLETRYPLGLFRAWSYVEPEARCLVYPRPERSPLPAPAPDAEAGAARAQALGNDDFS
                     GLRAYQLQDSPRHVAWKAVARSDDMLTKQFAGAAGIELWLDWERMAPNLDAEQRLSRL
                     AGWVLAAEQAGARYGLRLPARAMAPDRGDAHCAACLQALALHGVP"
     gene            complement(2754..3362)
                     /locus_tag="AC16O9_00015"
     CDS             complement(2754..3362)
                     /locus_tag="AC16O9_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6515036.1"
                     /translation="MRRTGLQAAVLAAALVHAAPAAAGAQVYEELAASVRHSLAAALS
                     DRSVPELAFREREDAHKWLFEMSRRLEKRVPDRKQRLELLRTAHFEALRAGLDPQLVL
                     GIIEVESRFRKYAVSRAGARGYMQVMPFWIKLIGSKRDNLFHLRTNLRYGCVILRHYL
                     DMENGDYFRALGRYNGSLGKPDYPRAVLAAWKVRWGYKGATS"
     gene            complement(3359..5068)
                     /locus_tag="AC16O9_00020"
     CDS             complement(3359..5068)
                     /locus_tag="AC16O9_00020"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR6515037.1"
                     /translation="MRTTRFHLGTIKEAPADAELVSHRLMIRAGYIRRLAAGIYTWLP
                     LGMRALRKVETVVREEMNRAGALELSMPAVQPAELWQESGRWQAYGPELLRFKDRHER
                     DFVIQPTSEEVITDLARAELKSYRQLPLHFYQIQTKFRDERRPRFGVMRGREFVMKDG
                     YSFHVDYADLEREYRNMYDTYTRIFTRLGLRFRAVAADTGAIGGTGSHEFQVLADSGE
                     DAIAYSAESDYAANIDLAEALAPAAARPAALAPMRKVPTPGKTRCEDVAAQLALPLAR
                     TVKALAVMQEAQFVLLLLRGDHELNEVKTQKAIGAFRFASEAEIRQALGSPPGYIGPV
                     GVAPQVRVVADRSVAAMGDFVSGANEEGFHLAGVNWGRDLPEPSLVADIRNVMEGDPS
                     PDGQGSLRIQRGIEVGHIFQLRTKYSEALGAKVLDAKGETRPLEMGCYGIGVTRVVAA
                     AIEQHHDARGIALPAALAPFAACLVPIAYGKSEKVRAAADRLYAELQQAGIEVLMDDR
                     DERPGVLFADMDLIGIPHRIVLSERGLAAGDAEYKGRRDAEARNVPLAGLAAFLKERI
                     AAA"
     gene            5144..5659
                     /locus_tag="AC16O9_00025"
     CDS             5144..5659
                     /locus_tag="AC16O9_00025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR6515038.1"
                     /translation="MLDRDGYRPNVGIILANGRNEVFWGKRVHQHAWQFPQGGIKHGE
                     TPERAMYRELDEEVGLKADHVRILGRTRHWLRYEVPEKWVRRDWRGSYRGQKQIWYLL
                     RLVGRDCDVRLRASAKPEFDAWRWHDYWVPLEAVIEFKREVYRQALAELHRYLGADRR
                     RQPRPEATETR"
     gene            5917..>6209
                     /locus_tag="AC16O9_00030"
     CDS             5917..>6209
                     /locus_tag="AC16O9_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR6515039.1"
                     /translation="MKALKGTKTHQHLKDAFAGESQANRRYLYFAAKADVEGQNDVSA
                     LFRSTAEGETGHAHGHLEYLEQVGDPATGLPIGRTRDNLKSAVAGETHEYTDM"
BASE COUNT          948 a         2172 c         2253 g          836 t
ORIGIN      
        1 gcgcgcgcca cgccgcgggc gcggagcttg ccgcagaacg cctgccaggc gcgctgcacc
       61 gggtcggggc gcgcgaggcg ccgcagcgac cacacgagca gcacgagcgt gaacgcgccg
      121 aggatcgacg ccatcgccgc cgtgagcttg cgccagtcgg cgtcgcgcat gccgatgcgc
      181 gacatcaggt cgcgctggcg ctcggcgccg tagccgagta cccaaacgtt ccagcggtgc
      241 gcgagcgcct cccagttgct gcgcaggccg cgcagccatt cgagctgcgg ccgcatcagc
      301 aggggcaccg ggtcgcccgc gcgcaccgcg cgggcgaggc cggcatcgat ccggcccggc
      361 acggcggccg cggtcgggtc ggcgcgcacc cagccgcgcc ccgccaggtg cacttccgcc
      421 caggcatgcg cgtcggattg gcgcacgctg atgatccggt ccacgtaatt cacctcgccg
      481 ccctggtagc cggtgaccac gcgcgccggc acgcccgcgg cgcgcatcag gaacacgaac
      541 gccgaggaga aatgctcgca gaagccctcg cgcgtgtcga acaggaactc gtccacggtg
      601 tcgcgcccga ggagcggcgg ttccagcgtg tagccgaggc ggctgtcgcg gaagaactcg
      661 accgcgcgct gcagcacctc caggtcgctt cgggcgccgc gttgccattc ggcggccagc
      721 gcgcgcgcgc ggggattgcc ggacacgggc agggagagcg cgcggcgcag gctgtccggg
      781 cgctcgaacg gctcgggctc ggggcgggcg agcgacgcgg cgtcgtagcg cagtcgcgtg
      841 cgcaccggcg aggtggccac caccatgcca tcgttcgtat agcgcgcgcg caccggcagc
      901 cgcgtgacgc tttccagtgc gaacatccag gtgcggttgt gcggctccag ggtcaccgtg
      961 tactcgtagc gcccgccgcc ctggagcgcg tcgaacttct gcagccagcc ggcacccatg
     1021 cgccaggtac ggccgtcgaa gtcccacagt acgggcccgc gccagtagcg ctcgcggggc
     1081 ggcggcggct cgccttcgaa ctcggcgcga aaggcgatgg catcggattg cgcgagctgg
     1141 gccacggcgc ccggcgtcat ggtgtcggag agtccggtca tggcggcgta ggcgtcctgc
     1201 ggcaggcccc acaacggccc ctgcacgcgc ggaaacagca ggaacaggat cagcgccgcg
     1261 ggcgccgcgt gcgcgagcag tacccccgcg ctgcgcaggt tggcgagcgc cggacgctgc
     1321 ggcgcggcga ggcccaccag cgtgccggtg acggcgagca gcgccagcgc catgagcagc
     1381 gccgtcggga tgctctgcgt gtacagcagg ttggtcatca ccaggaagta gcacagaaag
     1441 gcgaccacgg cggcgtcgcg gtgggtgcgc atctcggcga gcttcagccc cgagaacagc
     1501 accagcagca cgatccccga ggtgcgcccg aacagcgtgc ggtactcgag gtacacggcg
     1561 ccgacggcga gcagcgccag tgcgaggacc agccagcgcg agggcagcgc cgagcgctcg
     1621 agcgcgatgc gcgcgcgcca ggcgtacagg cccagcacca agagcgtcag ccaccagggc
     1681 atgcgcagcg cgtgcggcgc ggcgaccagc acgagcgagg cgagcagccc ggccagatcg
     1741 cgcggcgcga ccgcgcgcag ctcgggcgcg agcgggtgcg ggacgctcat ggcacgccgt
     1801 gcagcgcgag cgcctgcagg caggctgcgc agtgcgcgtc gccgcgatcg ggcgccatcg
     1861 cgcgcgccgg gagccgcagc ccgtagcgcg cgccggcctg ctcggcggcc agcacccagc
     1921 cggcgagccg cgacagacgc tgctcggcgt ccaggttcgg ggccatgcgc tcccagtcga
     1981 gccacagctc gatgccggcg gcgccggcga actgcttggt cagcatgtcg tccgagcgcg
     2041 ccacggcctt ccacgccaca tggcgcggcg agtcctgcaa ctgataggcg cgcagccccg
     2101 aaaaatcatc gttgccgagg gcctgcgcgc gcgccgcgcc cgcttcggcg tccggtgccg
     2161 gcgccggcag cgggctgcgc tcggggcgcg gatacaccag gcaacgcgcc tcgggctcga
     2221 cgtagctcca ggcgcggaac aacccgagcg gatagcgcgt ctccagcatg acgcgggtga
     2281 gcggcagcca gccgcgccgc gcggcaggca ccggcagcac cgcctcgccg accgcgccgg
     2341 gcgccagatc gagcaccacc tgggcgccgc tcgcctggtg gcgcacaagg atcgccggcc
     2401 ggtcgtagcg cgcgcggttg tcgaggtaca ggcggaactg tgccgcctcg ccggcgaaca
     2461 ccggcgcggc gcgcccggcg ctcaccgtca ggcgcgcgag gttgcgggtg gtgtgcacca
     2521 tgccgacgag gccgaggccg gcgaggagga acgtgagtgc gaagccgagc tgcagcgcat
     2581 agttgatcga gccgacgagc aggatgccga gcgtggcgcc gtagaaccag cccaggcgcg
     2641 tcggcacgat gtagacgcgg cgctggccga gcgtgatggc gccgcgctcg ggcgcgcggt
     2701 agccgtagaa ccagtcgcgc aggcgcgcgt cgttgagcag ggcgcggatc atgtcagctg
     2761 gtcgcgccct tgtagcccca gcgcactttc catgccgcca gcaccgcgcg cgggtagtcg
     2821 ggcttgccga ggctgccgtt gtagcggccg agcgcgcgaa aatagtcgcc gttctccatg
     2881 tcgagatagt ggcgcaggat gacgcagccg tagcgcaggt tggtgcgcag gtggaacagg
     2941 ttgtcgcgct tggagccgat cagcttgatc cagaacggca tcacctgcat gtagccgcgg
     3001 gcgccggcgc gcgataccgc gtacttgcga aagcggcttt ccacctcgat gatgcccagc
     3061 accagttgcg ggtcgagtcc cgcgcgcagc gcctcgaaat gcgcggtgcg cagcagctcc
     3121 aggcgctgct tgcggtcggg cacgcgcttt tcgagccggc gcgacatctc gaacagccat
     3181 ttgtgcgcgt cctcgcgctc gcgaaacgcc agctccggca ccgagcggtc gctcagcgcg
     3241 gccgcaaggc tgtggcgcac gctggccgcg agctcctcgt agacctgtgc gccggcggcg
     3301 gccggcgctg cgtgcacgag cgccgcggcg agcaccgccg cttgcaggcc ggtgcgcctc
     3361 atgcggctgc gatccgctcc ttgaggaagg cggcgagacc cgcgagcggc acattgcgcg
     3421 cctcggcatc gcgccgcccc ttgtactcgg cgtccccggc cgccaggccg cgctccgaga
     3481 gcacaatgcg atgcgggatg ccgatcaaat ccatgtctgc gaacagcacc cccgggcgct
     3541 cgtcgcggtc gtccatcagc acctcgatgc cggcctgctg cagctccgcg tacaggcggt
     3601 cggccgcggc gcgcaccttc tcgctcttgc cgtaggcgat gggcaccagg caggccgcaa
     3661 aaggcgccag cgccgccggc agcgcgatgc cgcgcgcgtc gtggtgctgc tcgatcgccg
     3721 ccgccaccac gcgcgtcacg ccgatgccgt agcagcccat ctccagcggc cgcgtctcgc
     3781 ccttggcgtc gagcaccttc gcgccgagcg cttcggagta cttggtgcgc agctggaaga
     3841 tgtggccaac ctcgatcccg cgctggatgc gcaggctgcc ctggccgtcg gggctcggat
     3901 cgccctccat gacgtttctg atgtcggcca cgagcgatgg ttccggcagg tcgcggcccc
     3961 agttcacgcc ggcgaggtga aatccctcct cgttggcgcc gctgacgaag tcgcccattg
     4021 ccgccacgct gcggtcggcg accacgcgta cctgcggcgc cacgcccacc ggcccgatgt
     4081 agccgggggg cgagccgagg gcctggcgta tctcggcctc gctcgcaaag cgaaaagcgc
     4141 cgatcgcctt ttgcgttttc acctcgttca gttcgtggtc gccgcgcagc agcagcagca
     4201 cgaattgcgc ctcctgcatt acggcgagcg ccttcaccgt gcgcgcaagc ggcagcgcga
     4261 gctgcgcggc gacgtcctcg cagcgggtct tgccgggcgt gggcaccttg cgcatcggcg
     4321 ccagggccgc gggccgcgcc gcggccggcg cgagcgcctc ggcgaggtcg atgttcgcgg
     4381 cgtagtcgga ctcggcgctg taggcgatgg cgtcctcgcc ggaatcggcc agcacctgga
     4441 actcgtgcga gccggtgccg ccgatggcgc cggtgtcggc tgccacggcg cgaaagcgca
     4501 gcccgaggcg cgtgaagatg cgcgtgtagg tgtcgtacat gttgcggtac tcgcgctcca
     4561 ggtcggcgta gtcgacgtgg aaagagtagc cgtccttcat cacgaattcg cgcccgcgca
     4621 tgacgccaaa gcgcggccgg cgctcgtcgc ggaacttggt ctggatctgg tagaagtgca
     4681 gcggcagctg gcggtagctc ttcagctcgg cgcgcgccag gtccgtgatc acctcctcgg
     4741 aggtgggctg gatgacgaag tcgcgctcgt ggcggtcctt gaagcgcagc agctccgggc
     4801 cgtacgcctg ccagcgcccg gactcctgcc acagctcggc cggctgcacc gcgggcatcg
     4861 acagctcgag cgcgcccgcc cggttcatct cctcgcggac cacggtctcc accttgcgca
     4921 gcgcgcgcat gccaagcggc agccaggtgt agatgcccgc cgccaggcgc cggatgtagc
     4981 cggcgcggat catcaggcga tggctgacga gctccgcatc ggcgggcgcc tccttgatcg
     5041 tgccaagatg gaatcgcgtt gtgcgcatcg gtcgctcgcc gcgttgtgtt tgcggttcgc
     5101 ctgtggaaaa atgcgagtgg attctaactt aggtggtgtg gccatgctcg accgggacgg
     5161 gtatcgcccg aacgtcggca tcatcctcgc caacggccgc aacgaggtgt tctggggcaa
     5221 gcgcgtccac cagcacgcgt ggcagtttcc ccagggcggc atcaagcacg gcgagacgcc
     5281 ggagcgggcg atgtaccgcg aactcgatga ggaggttggc ctcaaggcgg atcacgtccg
     5341 cattctcggg cgcacgcgcc actggctgcg ctacgaggtg cccgagaaat gggtgcggcg
     5401 cgactggcgc ggctcctacc gcggccagaa gcagatctgg tacctgctgc ggctggtcgg
     5461 tcgcgactgc gacgtccgcc tgcgcgccag cgccaagccc gagttcgacg cctggcgctg
     5521 gcacgactat tgggtgccgc tcgaggcggt gatcgagttc aagcgcgagg tctaccgcca
     5581 ggcgctggcc gagctgcacc gctatctcgg ggcggaccgg cgccgccagc cgcgtcccga
     5641 ggccaccgag acgcgctgag cgcgccgccg ccccggcaaa ggaggtgtcg tggacaggca
     5701 gtaccagggt cttccgatcc gcgagctggg cgccgcagcg ccgcagccgg gcgaggtggc
     5761 acgcatcttc gcagagatcg aggcagcgct cgggcactga gcgactcata gcgcaaggca
     5821 attgccggaa ttgcaaaaat cgatttgatt gatcggtgtc gcggcggtac cttcgctgcg
     5881 ccaggaacga cgcattcacg ctcaaaggag gctttcatga aggcactcaa gggaacgaag
     5941 acgcaccagc acctgaagga cgcgtttgcc ggcgaatcgc aggccaatcg tcgctacctg
     6001 tacttcgccg cgaaagccga cgtggaaggc cagaacgacg tgtcggcgct gttccgctcg
     6061 accgcggagg gcgagaccgg ccacgcgcac ggccatctcg agtacctgga gcaggtgggc
     6121 gacccggcga ctggcctgcc gatcggccgc acgcgcgaca atctgaagtc ggcggtggcg
     6181 ggcgagaccc acgagtacac ggacatgta
//
LOCUS       DBNHKE010000002        14226 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_846881, whole genome shotgun sequence.
ACCESSION   DBNHKE010000002 DBNHKE010000000
VERSION     DBNHKE010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_846881"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="AC16O9_00035"
     CDS             <1..1492
                     /locus_tag="AC16O9_00035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR6515040.1"
                     /translation="AGVSRALPGCAQGAPRFAGYPFGLGVASGYPHPGGVVLWTRLTG
                     ELPPVALPVRWEIAADESMSKPVAAGSAVAEPDWAHSLRVEAKGLEPDRWYWYRFTAG
                     DAVSPVGRTRTAPRPDAAVERLRFAFASCQQYEQGYFGAHRHIAADAPDLVAFLGDYI
                     YESSWGRDHVRKHTGGEPYTLEDYRARYALYKSDPDLQAAHAACPWIVTWDDHEVDND
                     YADDRPEDGMPREQFLARRAAAYRAFYENMPLPPRMAPRGPDMRLHTQLGWGRLGRFF
                     VLDGRQYRSWQACVRRGRRGGSNLVDAEECRALSDPARSMLGRAQERWLEGALGDSRA
                     QWSFLAQQTRMAQLDQKPGPGRRAWTDAWDGYAAARARLLGFLEQRRIANPVVLSGDA
                     HLFSVADLKRDFDDPRSPVVASEFLGTSITSQGWPQRRLDELRADNPHVKLMDSRFRG
                     YVRAEASARESRFELRAMASVGEPGAGCRTHAIFVVEDGQPGPQRA"
     gene            complement(1489..1878)
                     /locus_tag="AC16O9_00040"
     CDS             complement(1489..1878)
                     /locus_tag="AC16O9_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR6515041.1"
                     /translation="MASIEGRTRRLASLPLPAEQERELLRLLDEAGIVHREARSKSRL
                     FGGDAIWVAEADYPRAREILEREATQYAAAAREKWQAQWRDEHGGSYLRWLCSRLRHA
                     TMEDVVRALFLIVLVALMLLYPLSLMR"
     gene            complement(1886..2623)
                     /locus_tag="AC16O9_00045"
     CDS             complement(1886..2623)
                     /locus_tag="AC16O9_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6515042.1"
                     /translation="MLQAVHRFLSGQSQTRISLGALILVAVIGATDHVTGYELSASTW
                     LLVDYTSGHAYSYFAIPFWNAGVCLGIFLIVAHLLSNLKSALAQQDGLTGITNARTFR
                     QRDELLAHLAIRHGRPLTLGYVDLDGFKGVNDNLGHEVGDRVLRAVAAELSRRLRTSD
                     IVGRLGGDEFALLLTETDLNGARTLFADVRERLLAVACGTAGRSASASAWPFSSRPRR
                     ALRKPSSTPTHSCTRPSMTLIALHIVR"
     gene            complement(2623..3480)
                     /locus_tag="AC16O9_00050"
     CDS             complement(2623..3480)
                     /locus_tag="AC16O9_00050"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6515043.1"
                     /translation="MQVGDFPDPRYLIEARELRERLDALTLLDLRPAEDFALGHIPGS
                     RHLDIYGVSLNDSSEAPLNAFLAIFRVLFGARGVTADKPAVVYDHESGERAARAVWLL
                     AVLGHPDVRLLDGGTQAWTGAGGALVQVRQAPAPVDPAKAPPTAPPFRGKIDLGLLAT
                     RFDVHEAIGDAGKVILDVRRESEYRGTEKRARRVGTIPGAVHVFWREHLDARGAYRPA
                     EEIRRLYESKGVTPDKTIIPLCQGGYRSASTFIALKSLGYPRVKNYVSSWGEWGNRED
                     SKIVVPSGE"
     gene            complement(3471..3788)
                     /locus_tag="AC16O9_00055"
     CDS             complement(3471..3788)
                     /locus_tag="AC16O9_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515044.1"
                     /translation="MKLMALACVALLAACTHASVRYDSGGGTVTTSASVSAGYRGSSA
                     AAWALIGIGLIAAELAGSEAARERGLEPRTAPAQPMDAQRQVNEVDCTRPIENWSANL
                     RCK"
     gene            complement(3785..4720)
                     /locus_tag="AC16O9_00060"
     CDS             complement(3785..4720)
                     /locus_tag="AC16O9_00060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6515045.1"
                     /translation="MSADVRRAAEVLKRGGLVGFPTETVYGLGADACSREAVARLYAA
                     KRRPADHPVIVHFADAKQAFTWAREVPDGARKLAAKFWPGPLTLVLKRSKKAGDFVTG
                     GQDTIGVRVPSHPVAQALLQAFGGGIAAPSANRFGAVSATTAAHVKQDLGKDVEVVLD
                     GGPSEVGIESAIVDLSGAQPVLLRPGKISRLELEEVLGEPVSERRPDSPRHSGGLERH
                     YAPQTPARLVPPHELDREIARLRDKVAVLAFSRPDERVEYWLRMPREPLGYAQRLYAA
                     LRELDGAGCESILVEAPPETPEWAGVRDRLMKATA"
     gene            complement(4717..5196)
                     /gene="purE"
                     /locus_tag="AC16O9_00065"
     CDS             complement(4717..5196)
                     /gene="purE"
                     /locus_tag="AC16O9_00065"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR6515046.1"
                     /translation="MIGIVMGSANDWEVMKAAAKTLEEFGIAYDAKAMSAHRTPHAVG
                     EWAEGAAKRGLKAIIAAAGGAAHLAGVVAAHTTLPVLGVPMPSKHLQGLDSLLSTVQM
                     PKGIPVATFAIGEAGAANAALFAVALLALSDPAMAKKLDEFRKKQTEAVLKAAPLKL"
     gene            complement(5193..6086)
                     /locus_tag="AC16O9_00070"
     CDS             complement(5193..6086)
                     /locus_tag="AC16O9_00070"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR6515047.1"
                     /translation="MKNALYESSLKSLPRLGRGKVRDIYGVGEDKMLIVVSDRLSAFD
                     VVLPDPIPDKGRVLNEMANFWFGRLGHIVPNQLTGIDPLSVVRGEDEKAQVKDRAIVV
                     KKLKPLPIEAVVRGYLIGSGWKDYQNTGAVCGIALPKGLAQAQKLPEPIFTPATKATD
                     GHDENISFEETVKLIGKETAQKVRDIAIRLYKEASEYAATKGIIIADTKFEFGTDAQG
                     NLVLIDEVLTADSSRFWPADSYKVGVSPPSFDKQYVRDYLETLDWDKTPPAPKLPPEV
                     IARTSQKYREALERLTGRKLA"
     gene            complement(6083..7105)
                     /locus_tag="AC16O9_00075"
     CDS             complement(6083..7105)
                     /locus_tag="AC16O9_00075"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR6515048.1"
                     /translation="MDKNLLSKTAAAMVARGKGVLAADESSGTCEKRFKSVNVECTEE
                     NRRAYRGLLFATPGVEQYVSGVILFDETIRQKTADGAAFPAYLAGKGILPGIKVDKGA
                     MDLALCPGEKVTEGLDGLRGRLAEYFKLGARFAKWRAVITIGEGLPSHACLYANAHAL
                     ARYAALCQEASIVPMIEPEVLLDGNHSVERSEEVTEATLRATYAACAAHSVSVEHLIL
                     KTSMVVSGKDHPRQAPVDEVAERTVRVLKRTVPAAQPGIVFLSGGQSDLDATAHLNAM
                     NNMKGLPWPLTFSYSRALQNPALKAWSGKAANVAAAQKAFHHRAHMNGLAAQGKWKPE
                     LERQAA"
     gene            complement(7127..7615)
                     /locus_tag="AC16O9_00080"
     CDS             complement(7127..7615)
                     /locus_tag="AC16O9_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR6515049.1"
                     /translation="MRRPLLLVVFTALPLAAAAQGTHAIRLLTPDTALKAAQAALKKC
                     RDSGYQATIAVVDRMGVTQVLLRDRYAGAHTVHTAQAKAYSAVSFRTNTTELSEATQA
                     GRPQSGIRHLPGVAAVGGGLMIEAGGSLLGAIGVSGAPGGKEDDVCAAAGIDAIRDDI
                     EF"
     gene            complement(7648..8736)
                     /locus_tag="AC16O9_00085"
     CDS             complement(7648..8736)
                     /locus_tag="AC16O9_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016727121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR6515050.1"
                     /translation="MNEADPGQIAHTVAAGAFALAFIFGAVGNKTEFCTMGAVSDWVN
                     MGDTNRMRMWLLAIAVALLGSSALAAAGVVDLSKSIYPGPNFTWLSYIVGGFLFGVGM
                     TLGSGCGSKTLIRIGGGNLKSLVVFVFLGIAAYMTLRGLFGAFRVGVLERAAVTLPQG
                     QDLPSLLGWHRAIIAGLIGGALVVFCYARREFRQNFDYTLGGVVTGLVVVGGWYVSGV
                     VGYVAEHPETLQEAFIATNTGRMESFSFVSPMAFTLEYLMLWTDASKIITYGIASGLG
                     VIAGSAAYALGSGKFRWEGFRDAEDTANHVIGGILMGFGGITALGCTVGQAITGVSTL
                     ALGSFLTFAAIVAGSALTMKVQYWRMGG"
     gene            complement(8792..9880)
                     /locus_tag="AC16O9_00090"
     CDS             complement(8792..9880)
                     /locus_tag="AC16O9_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012800.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR6515051.1"
                     /translation="MSAALMSSILKRIATGPELSKDISREEAREGMRLVLEGAVDPVQ
                     AGVFLIALRMKRETDDENRGLLEAIREATLRATAQVDEVLDLGDPYDGYNRTLPAAPF
                     LPAVLAACGVPTVSHGVESMGPKYGVTHRQVLAAALAPVDLTPEQAAARLADPRRGWA
                     YVDQKAFCPKLYALAHLRTLIVKRPAITTAEVLAGPVRGRRRTHLVTGFVHKPYARIY
                     ALLARHAGFDSALIVRGVEGGVIPGLTQTGKVFRVLGDAEEAPVEFQPAELGIEQSVR
                     APRVPGAREDEEGAARLDTAVLAKAAAQAGVAALQGQAGATRDCLVVGAALCLWHLGR
                     HASLREAADAVRRALDGGEALARLHQAK"
     gene            complement(9877..10200)
                     /locus_tag="AC16O9_00095"
     CDS             complement(9877..10200)
                     /locus_tag="AC16O9_00095"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6515052.1"
                     /translation="MTFVEIARAGELREGTMQRFDVAGRRILLANVGGRLYAVDDTCT
                     HEEASLSTGVLKGGHVKCPLHNSRFDVRTGEALEEPAEEPLRTYPVREEGGRVLIGMP
                     GEGAA"
     gene            complement(10197..11582)
                     /gene="cysG"
                     /locus_tag="AC16O9_00100"
     CDS             complement(10197..11582)
                     /gene="cysG"
                     /locus_tag="AC16O9_00100"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039051507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HIR6515053.1"
                     /translation="MEFLPIFLKVRGAPCLVVGGGEVAARKCALLARAGARVTVLAPE
                     LGKALEAERAAGRIAHRAERFEDAHVQGYAAVIAATSDQAVNRSVAAAAKARLIPVNV
                     VDQPALCSFILPSIIERAPLIVAVSSGGASPVLARLLRARLESMIPAAYGRLAALAAE
                     FRERVKARFQGAERRRFWERALQGPIAELMLAGRDAEAREALAAAIDDTRLAFTGGEV
                     ALVGAGPGDPDLLTFRALRLMQQADVVVYDRLVSQPILDLARLEAERIYAGKERAMHT
                     LPQEDINQLLVRLAKAGKRVVRLKGGDPFIFGRGGEEIDTLAAEGIPFQVVPGVTAAA
                     GCASYAGIPLTHRDHAQSVVFVTGHLRDGSLNLNWRALAQPQQTIVFYMGLLAVDQLC
                     ARLAEHGLPAGTPAALVQAGTTPQQRVLTGTLATLPGLVEASEVKAPTLIIVGEVVRL
                     RERLKWFEPVA"
     gene            11614..13053
                     /locus_tag="AC16O9_00105"
     CDS             11614..13053
                     /locus_tag="AC16O9_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6515054.1"
                     /translation="MPRYLTLSRAARLVGVRRGVLQSKIRNGELAAFEGMVAEADLLR
                     AYPEAHLEDNTTLERLEAIKEAAFARRVRERVLPSAEVLMARLTELGRERAAADAELA
                     RTRAILAELTQRLRASADGTRLADWVEARLAAPAPGGPALLAHESLLRIMSAHVQVQP
                     SGREFFVDGNDSLLEAALRAGLSLDYGCSVGNCGKCKARVVSGQVHRLRHSDYALGAA
                     EKAAGVVLMCCHTAATDVVLEAREAHGAADMPLQTIDTRVKVVSPMGEHMRLLHLQTP
                     RTQRLRFLAGQSVRLSLGEDAAASLPVASCPCDERNLHFHVPRRAGDAFAERVFAGLK
                     GVDTVRIEGPRGEFVLDEDSIRPLVFVAFDTGFAPVKSLIEHAMALDASESLHLYWIA
                     TGAGGHYLDNLCRAWNDALDNFRYVPLSAAGGRADEAALGALLKDHPRFDECDVYVAG
                     PTPAAQAAEFALLERGLPRAQLRLGTLEG"
     gene            13060..>14226
                     /locus_tag="AC16O9_00110"
     CDS             13060..>14226
                     /locus_tag="AC16O9_00110"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6515055.1"
                     /translation="MSDRASRQAELLAQAGRTLAGGGLGLFVLPPELNLVVERGQGSR
                     VWDVAGREYIDYHLGSGPALLGHGHPAISAAIAARLDKGTTYYFLNEPEIELAQRLID
                     AVACGERVHYAGSGTEATFYALRIARAHTGRDKILKFEGAWHGMHDYGLWGTVPSTPS
                     DYPRARPDSVGVPRQAGETVLVAPFNDAERAVALIEAHARELAAVIVEPLQRVLRPEP
                     GFLEALRAVTQRHGIVLVFDEIVTGFRIAWGGAQERYGVVPDLACYGKAVSGGLPMAM
                     IVGRAEVLAVLDARSRPREQVVWATHTLNGNPICAAAGCAALDVLAKPGVYDHLHRLG
                     ARLRAGIVESGARHGFAVQAPGEDAVFGVRFTGRRPLRNWMDLTTADKDLGWRWA"
BASE COUNT         2017 a         5100 c         5004 g         2105 t
ORIGIN      
        1 cgcgggcgtt tcgcgcgcgc tgcccggctg cgcgcaaggc gcgccgcgct tcgcgggcta
       61 cccgttcggc ctcggcgtcg cctcgggcta cccgcacccg ggcggcgtgg tgctgtggac
      121 gcggctcacc ggcgagttgc cgcccgtggc gctgccggtg cgctgggaga tcgcggcgga
      181 cgagtcgatg tcgaagcctg tcgcagcggg aagcgccgtg gccgagcccg attgggcgca
      241 ctcgctgcgc gtggaggcga agggcctcga gcccgaccgc tggtactggt accgcttcac
      301 ggccggcgac gcggtaagtc cggtcggtcg cacgcgcacc gcgccgcgac cggacgccgc
      361 ggtcgagcgg ctgcgcttcg cgttcgcctc ctgccagcag tacgagcagg gctacttcgg
      421 cgcgcaccgg catattgccg ctgatgcgcc ggacctcgtc gcctttctcg gcgactacat
      481 ctacgaatcg tcctggggcc gcgatcacgt caggaagcac accggcggcg agccctacac
      541 gctcgaggac taccgggcgc gctatgcgct ctacaagtcc gatcccgacc tgcaggccgc
      601 gcacgccgcc tgcccgtgga tcgtcacctg ggacgaccac gaggtcgaca acgactacgc
      661 ggacgaccgg cccgaggacg gcatgccgcg cgagcagttt ctcgcgcgcc gcgcggcggc
      721 gtaccgcgcg ttctacgaga acatgccgct gccgccgcgc atggcgccgc gcggcccgga
      781 catgcggctg catacgcagc tcggctgggg aaggctcggg cgcttcttcg ttctggacgg
      841 ccgccagtac cgctcgtggc aggcgtgcgt gcggcgcggc cggcgcggcg gctcgaacct
      901 cgtcgacgcc gaggagtgtc gcgcgctgtc cgacccggcg cgctcgatgc tcgggcgcgc
      961 gcaggagcgc tggctggaag gcgcgctcgg cgactcgcgg gcgcagtgga gcttcctcgc
     1021 gcagcagacg cgcatggcgc agctcgacca gaagcccggg cccggacggc gcgcctggac
     1081 cgacgcctgg gatggctacg ccgcagcgcg cgcgcgcctg ctcgggttcc tcgagcagcg
     1141 gcgcatcgcc aatcccgtcg tgctgtcggg cgatgcgcac ctgttcagcg tcgccgacct
     1201 gaagcgcgac ttcgacgatc cgcgctcacc cgtggtggcg agcgagttcc tcggcacctc
     1261 gatcacctcg cagggctggc cgcagcgccg gctcgatgag ctgcgggccg acaacccgca
     1321 cgtaaagctc atggactcgc gctttcgcgg ctacgtgcgc gcggaggcct cggcaaggga
     1381 gtcgcgcttc gagctgcgcg cgatggcgtc ggtgggcgag cccggcgccg gctgccgcac
     1441 gcacgcgata ttcgtggtgg aggatggcca gcccgggccg cagcgcgcct agcgcatgag
     1501 gctcagggga taaaggagca tcagcgccac gagcacgatg agaaagagcg cgcgcaccac
     1561 gtcttccatg gtcgcgtgcc gcaggcggct gcacagccag cgcaggtacg agccgccgtg
     1621 ctcgtcgcgc cattgcgcct gccacttctc gcgcgctgcg gcggcgtact gcgtcgcttc
     1681 ccgctccagg atctccctcg ccctcgggta gtccgcttcg gcgacccaga tcgcgtcgcc
     1741 gccgaagagc cgcgacttcg agcgcgcctc gcggtgcacg atgcccgctt cgtcgagcag
     1801 gcgcaggagc tcgcgctcct gctcggccgg gagcggcagg ctggcaaggc gccgggtccg
     1861 cccttcgatc gacgccatca ggtcgctacc ggacgatgtg cagcgcaatg agcgtcatgc
     1921 ttggccttgt acatgagtgc gtcggcgtgc ttgagggctt cctcaacgct ctccgggggc
     1981 ggctggaaaa cggccacgcc gatgctgaag ccgaccggcc agcggttccg caggcgacgg
     2041 cgagcaggcg ttcgcggacg tccgcgaaca gggtgcgggc gccgttcaaa tcggtttcgg
     2101 tgagaagcag cgcgaactcg tcgccgccca agcgcccgac gatatccgag gtacgcagcc
     2161 tgcgggacaa ctcggccgcg accgcccgca gcacgcgatc gccgacctcg tggccaaggt
     2221 tgtcattgac gcctttgaag ccatccaggt cgacatagcc gagcgtcagc ggccgcccat
     2281 gccggatcgc caggtgcgcc agcagctcgt cgcgttgcct gaacgtgcgt gcgttcgtga
     2341 tgcccgtcag tccatcctgc tgcgcgagcg cgctcttgag attggacagc agatgggcaa
     2401 cgatgaggaa gattcccagg cagacgccgg cgttccagaa cgggatggcg aaatatgagt
     2461 acgcatggcc cgaggtgtag tccacgagca accacgtcga ggccgagagc tcgtatccgg
     2521 tgacgtgatc cgtggcgccg atgacggcga ccagaatcag cgcgccaagc gagatccggg
     2581 tctgcgactg cccagacaga aaccggtgaa cggcctggag cattactctc ccgacggcac
     2641 gacgatcttc gaatcctcgc ggttacccca ctcgccccat gaagacacgt agttctttac
     2701 ccgcggatac cccagcgact tcagcgcgat gaacgtgctc gccgagcggt agccgccctg
     2761 gcacagcgga atgatcgtct tgtccggcgt cacgcccttc gactcgtaca accggcggat
     2821 ctcttcggcc ggccggtacg cgccgcgcgc atccaggtgc tcgcgccaga agacgtgcac
     2881 cgcgcccgga atcgtcccga cgcgccgcgc ccgcttttcc gtgccccggt attcggactc
     2941 gcgccgcacg tcgaggatca ccttgcccgc atcgccgatt gcctcgtgca catcgaagcg
     3001 cgtcgccagc agcccgagat cgatcttgcc ccggaaaggc ggcgcggtcg gcggtgcctt
     3061 tgccggatcg accggcgccg gcgcctgtct cacctgcacc agcgccccgc ccgcccccgt
     3121 ccacgcctgc gtcccgccgt cgagcaggcg cacatcgggg tgcccgagca ccgccagcag
     3181 ccacaccgcg cgcgccgcgc gctcgccgga ctcgtggtcg tagacgacgg cgggcttgtc
     3241 cgccgtcacg ccgcgcgcgc cgaacagcac gcggaagatg gcgaggaacg cgttcagcgg
     3301 cgcttccgaa gaatcgttca ggctgacgcc gtagatgtcg agatggcggc tgcccgggat
     3361 atggccgagc gcaaagtcct ccgcgggccg caggtcgagc agcgtcaatg cgtcgagccg
     3421 ctcgcgcagt tcgcgcgcct cgatcaggta gcgcgggtcg gggaagtccc ctacttgcac
     3481 ctgaggttcg ccgaccagtt ctcgatcggc cgggtgcaat ccacctcgtt cacctggcgc
     3541 tgcgcatcca tcggctgcgc cggcgccgtc cgcggctcga gcccgcgctc gcgcgccgcc
     3601 tcgctcccgg cgagctccgc cgcgatcagg ccgatgccga tcagcgccca ggcggcggcg
     3661 ctgctgccgc ggtagccggc cgagacgctg gcgctggtgg tcacggtgcc gccgccggag
     3721 tcgtagcgca cgctggcgtg cgtgcatgcg gcgagcagcg caacgcaagc cagcgccatt
     3781 agtttcatgc cgtcgccttc atcagccggt cccgcacccc ggcccattcc ggggtctcgg
     3841 gcggtgcttc gacgaggata ctctcgcacc ccgcgccgtc caactcgcgc agcgcggcgt
     3901 acaggcgctg cgcgtagccc agcggctcgc gcggcatgcg cagccagtac tccacgcgct
     3961 cgtcgggccg cgagaaggcc agcaccgcga ccttgtcgcg cagacgcgcg atctcgcgat
     4021 cgagctcgtg cggcggcacg agccgtgccg gcgtctgcgg tgcgtagtgg cgctcgaggc
     4081 cgcccgaatg acgtggcgag tcggggcgcc gttcggacac cggctcgccg agcacctcct
     4141 cgagctcgag gcgcgagatc tttccgggcc gcagcagcac gggctgcgcg cccgagaggt
     4201 cgacgatggc ggattcgatg ccgacctcgc tcggcccgcc gtcgagaacg acctcgacgt
     4261 ctttgccgag gtcctgcttc acgtgcgcgg cggtagtcgc cgagaccgcg ccaaagcggt
     4321 tcgcggaagg cgcggcgatg ccgccgccga aggcctgcag caatgcctgc gccaccgggt
     4381 gcgagggcac gcgcacgccg atcgtgtcct ggccgccggt gacgaagtcg cccgccttct
     4441 tcgatcgctt cagtaccagg gtgagcgggc cgggccagaa tttcgccgcg agtttcctcg
     4501 caccgtcggg aacctcgcgc gcccaggtga acgcctgctt ggcgtcggcg aaatgcacga
     4561 tcaccggatg gtccgcgggc cggcgcttcg ctgcgtagag gcgcgccacg gcctcgcggc
     4621 tgcaggcatc cgcccccagc ccgtacacgg tctcggtggg aaacccgacc agcccgccgc
     4681 gcttgagtac ttccgcggcg cgccggacgt ccgcgctcac agcttgagcg gcgcggcttt
     4741 caggaccgct tcggtctgct tcttgcggaa ttcgtccagc ttcttcgcca tcgccggatc
     4801 cgagagcgcg aggagcgcca ccgcgaaaag cgccgcgttc gccgcgccgg cctcgccaat
     4861 ggcgaaggtc gccaccggga tgcctttcgg catctgcacg gtcgatagca gcgaatccag
     4921 cccctgcagg tgcttggagg gcatcggcac gccgagcacc ggcaacgtcg tgtgcgccgc
     4981 gaccacgccg gcgaggtgcg cggcgccgcc ggcggcggcg atgatggcct tcaggccgcg
     5041 cttggccgcg ccttcggccc actcgccaac cgcgtgcggc gtgcggtgcg ccgacatcgc
     5101 tttcgcgtcg taggcgatgc cgaactcctc gagcgtcttc gccgccgcct tcatcacctc
     5161 ccagtcgttg gcggacccca tcacgatgcc gatcatgcga gctttctccc cgtcaggcgc
     5221 tcgagcgcct cgcggtactt ctgcgaggtc cgggcgatca cctccggcgg gagcttgggc
     5281 gccggcggcg tcttgtccca gtcgagcgtc tcgaggtagt cgcgtacata ctgcttgtcg
     5341 aaagatggtg ggctgacgcc caccttgtag gaatcggcgg gccagaagcg cgacgagtcc
     5401 gcggtgagca cctcgtcgat cagcacgagg ttgccctggg catcggtgcc gaactcgaac
     5461 ttggtgtcgg cgatgatgat gcccttcgtc gccgcgtact cggacgcctc cttatagagg
     5521 cggatcgcga tgtcgcgcac cttctgcgcc gtctccttgc cgatcagctt caccgtctcc
     5581 tcgaaggaga tgttctcgtc gtggccatcg gtcgccttgg tggcgggcgt gaagatcggc
     5641 tcggggagct tctgcgcctg cgcgaggcct ttcggcagcg cgatgccgca cacggcgccg
     5701 gtgttctggt agtccttcca ccccgagccg atgaggtagc cgcgcaccac cgcctcgatc
     5761 ggcaagggct tcagcttctt caccacgatg gcgcggtcct tcacctgcgc cttctcgtcc
     5821 tcgccccgca ccacgctcag cgggtcgatg ccggtaagct ggttgggcac gatgtggccg
     5881 agccgcccga accagaagtt cgccatttcg ttcagcacgc gccccttgtc ggggatcggg
     5941 tcgggcagca cgacgtcgaa cgccgagagc cggtcgctca ccacgatgag catcttgtct
     6001 tcgcccacgc cgtagatgtc cctcactttg ccgcgaccga ggcgcggcag gctcttcagg
     6061 ctcgattcgt agagtgcgtt tttcaagcgg cttgcctttc cagttcgggt ttccacttgc
     6121 cctgcgccgc caggccgttc atgtgcgcgc ggtgatggaa cgctttctgc gccgcggcaa
     6181 cgttggccgc cttgccgctc caggccttga gcgcgggatt ttgcagcgcg cgcgagtacg
     6241 agaacgtgag cggccagggc aggcccttca tgttgttcat ggcgttcagg tgcgcggtgg
     6301 cgtcgaggtc cgactgcccg cccgaaagga acacgatgcc gggctgcgcg gcgggcacgg
     6361 tgcgcttgag cacgcgcacg gtgcgctcgg cgacttcgtc caccggcgcc tggcgcgggt
     6421 ggtccttgcc cgagaccacc atgctggttt tcaggatgag gtgctcgacc gagacgctgt
     6481 gcgccgcgca cgcggcgtag gtggcgcgca gcgtcgcctc ggtcacctcc tcgctgcgct
     6541 ccacgctgtg gttgccgtcg agcagcacct cgggctcgat catcggcacg atggaggctt
     6601 cctggcacag cgccgcatag cgcgcgagcg cgtgtgcatt ggcgtacagg cacgcgtgcg
     6661 acggcaggcc ctcgccgatg gtgatcaccg cgcgccactt ggcgaagcgc gcgccgagct
     6721 tgaagtactc ggcaagccgt ccgcgcagcc catcgagccc ctcggtcacc ttttcgccgg
     6781 ggcacagcgc gaggtccatc gcgcccttgt ccaccttgat gccggggaga attcccttgc
     6841 cggcgagata cgcggggaac gccgcgccgt ccgccgtctt ctggcggatg gtctcgtcaa
     6901 agaggatcac gcccgagaca tactgctcca cgcccggcgt ggcgaacagc agcccgcggt
     6961 aggcgcggcg attctcctcc gtgcattcga cgttcaccga cttgaagcgc ttctcgcagg
     7021 tgccgctgga ttcgtcggcg gcgagcacgc ccttgccgcg cgcgaccatc gcggcggcgg
     7081 tcttggatag caggttcttg tccatcagcg cccctctgcg aaagaatcag aactcgatgt
     7141 cgtcgcggat cgcgtcgatg cccgccgcgg cgcagacgtc gtcttccttg cctcccggcg
     7201 cgcccgagac gccgatcgcg ccgagcagcg acccgcccgc ctcgatcatc agaccgccgc
     7261 ccaccgccgc cacccccggc aagtggcgga tgccgctctg cggccggccg gcctgggtcg
     7321 cctcggagag ctcggtggtg ttggtgcgaa agctcaccgc gctgtaggcc ttggcctgcg
     7381 cggtgtgcac ggtgtgcgcg cccgcgtagc ggtcgcgcag cagtacctgg gtcacgccca
     7441 tgcggtccac caccgcgatc gtcgcctggt agccgctgtc gcggcacttc ttgagcgctg
     7501 cctgtgcggc cttgagggcc gtgtcgggcg tgagcagcct gatggcgtgc gtgccctggg
     7561 cggcggcggc gagcggcagg gcggtgaaga ccacgagaag gagggggcgt cgcatgcgcc
     7621 ctattttacg acccccttgc gccgcggtca gccgcccatg cgccagtact ggaccttcat
     7681 ggtgagggcc gagcccgcca cgatcgccgc gaacgtcagg aacgagccca gcgccagggt
     7741 ggagacgccc gtgatggcct ggcccacggt gcagccgagc gccgtgatgc cgccgaagcc
     7801 catcaggatg ccgccgatga cgtggttggc cgtgtcttcg gcgtcgcgga agccttccca
     7861 gcggaacttg cccgagccga gcgcgtaggc cgccgagccc gcgatcaccc ccaggcccga
     7921 ggcgatgccg taggtgatga tcttcgaggc gtcggtccac agcatcaggt actcgagcgt
     7981 gaacgccatg ggcgagacga aggagaaaga ctccatgcgc ccggtgttgg tggcgataaa
     8041 ggcctcctgc agcgtctcgg ggtgctcggc gacgtagccg acgacgccgc tgacgtacca
     8101 gccgccgacc accaccaggc cggtcaccac gccgcccagg gtgtagtcga aattctggcg
     8161 gaactccctc ctcgcgtagc aaaagacaac gagcgcgccg ccgatcagcc cggcgatgat
     8221 cgcgcgatgc cacccgagca gcgagggcag atcctgtccc tgcggcagcg tcaccgcggc
     8281 gcgctccagc accccgacgc ggaacgcgcc gaacagcccc cgcagcgtca tgtacgccgc
     8341 gatgccgagg aacacgaaca ccaccagcga cttgaggttg ccgccgccga tgcggatcag
     8401 cgtcttcgag ccgcagcccg agccgagcgt catgcccacg ccgaagagaa acccgccgac
     8461 gatgtaggag agccaagtga agttcggccc cggatagatg gacttggaca ggtccaccac
     8521 gcccgcagcc gcgagcgcgc tcgagccgag cagcgccacc gcgatggcga gcagccacat
     8581 gcgcatgcgg ttggtgtcgc ccatgttcac ccagtccgac acggcgccca tggtgcagaa
     8641 ctcggtcttg ttgccgaccg cgccgaagat gaaggcgagc gcgaaggcgc cggcggcgac
     8701 ggtgtgggcg atctgcccgg ggtcagcttc gttcatgcgc gattttgttc cggcgcggcg
     8761 gcacggcaca ttgaagttgc ataagcgctc atcattttgc ctgatgcagc cgcgcgagcg
     8821 cttcgccccc gtcgagcgcg cggcgcaccg catcggcggc ctcgcgcagc gatgcatggc
     8881 gcccgaggtg ccacaggcag agcgcggcgc cgaccacgag gcagtcgcgc gtggcgcccg
     8941 cctgcccctg cagcgccgcc acgcctgcct gcgcggcggc cttcgcgagg accgcggtgt
     9001 cgagccgcgc cgcgccctcc tcgtcctcgc gcgccccggg gacgcgcggc gcgcgcaccg
     9061 actgctcgat gccgagctcg gccggctgga attccaccgg cgcctcctcg gcgtcgccga
     9121 gcacgcggaa caccttgccg gtctgcgtga ggcccgggat cacgccgccc tccacgccgc
     9181 gcacgatgag cgcggagtcg aagcccgcgt ggcgcgcgag cagcgcgtag atgcgcgcat
     9241 agggcttgtg cacgaagccg gtgacgaggt gcgtccggcg ccggccgcgc accggaccgg
     9301 cgagcacctc ggcggtggtg atggcggggc gcttgacgat gagcgtgcgc aggtgcgcaa
     9361 gcgcgtagag cttcgggcag aacgccttct ggtccacgta ggcccagccc ctgcgcgggt
     9421 cggcgagtcg cgcggccgcc tgctccggcg tgaggtctac cggcgcgagc gccgcggcga
     9481 gcacctgccg gtgcgtgacg ccgtacttgg gtcccatgct ctcgacgccg tgcgacaccg
     9541 tcggcacgcc gcaggcggcg agcaccgccg gcaggaacgg cgccgcgggc agcgtgcggt
     9601 tgtagccgtc gtagggatcg ccgaggtcga gcacctcgtc cacttgcgcg gtcgcgcgca
     9661 gcgtggcctc gcggatcgcc tcgagcaggc cgcggttctc gtcgtcggtc tcgcgcttca
     9721 tgcgtagcgc gatgaggaac acccccgcct gcaccgggtc caccgcgccc tccagcacga
     9781 ggcgcatgcc ctcgcgcgct tcctcgcgcg aaatgtcctt gctgagctcc ggcccggtgg
     9841 cgatgcgctt gaggatcgag ctcatcagcg ccgcgctcat gccgcaccct ccccgggcat
     9901 gccgatcagc acgcgcccgc cctcctcgcg taccgggtag gtgcgcagag gctcctcggc
     9961 gggctcttcc agcgcttcgc cggtgcgcac gtcgaagcgg ctgttgtgca acgggcactt
    10021 gacgtgcccg cccttcagca ccccggtgga gagcgaggcc tcctcgtgcg tgcaggtgtc
    10081 gtccacggca tagagccgcc cgccgacgtt ggcaagcagt atgcgacggc cggcaacatc
    10141 gaagcgctgc atcgtgcctt cgcgcagctc gcctgccctg gcgatctcga cgaatgtcat
    10201 gcgaccggct cgaaccactt caagcgctcg cgcagccgca ccacctcgcc gacgatgatg
    10261 agggtaggtg ccttcacctc gctcgcctcg acgaggccgg gcagcgttgc gagcgtcccc
    10321 gtcagcacgc gctgctgcgg cgtggtgccg gcctgcacca gcgcggcggg cgtgcccgcc
    10381 ggcaggccgt gctcggcgag gcgcgcgcag agctggtcaa ccgcgagcag gcccatgtag
    10441 aacacgatcg tctgctgcgg ctgggcaagc gcgcgccagt tgagattgag gctgccgtcc
    10501 cgcaagtgcc cggtcacgaa caccaccgac tgcgcatggt cgcggtgcgt gagcgggatg
    10561 cccgcgtagg acgcgcagcc cgcggccgcg gtgacgccgg gcaccacctg aaacgggatg
    10621 ccctcggcag cgagcgtgtc gatctcctcg ccgccgcgcc cgaagatgaa cggatcgccc
    10681 cctttcaggc gcacgacgcg cttgcccgcc tttgccaggc gcacgaggag ctgattgatg
    10741 tcctcctgcg gcagcgtgtg catggcgcgc tccttgccgg catagatgcg ctcggcctca
    10801 agccgcgcca gatccaggat cggctgcgac acgaggcggt cgtagacgac gacgtcggcc
    10861 tgctgcatga ggcgcagcgc gcggaacgtg agcaggtccg ggtcgcccgg gcccgcgccg
    10921 acgagcgcca cctcgccgcc ggtgaatgca aggcgcgtgt cgtcgatggc cgcggcgagc
    10981 gcctcgcgcg cctcggcgtc gcgcccggcg agcatcagct cggcgatcgg cccctgcagc
    11041 gcgcgctccc agaagcggcg ccgctcggcg ccctggaagc gcgccttcac gcgctcgcgg
    11101 aattccgcag cgagcgcggc gagccgcccg taggccgccg ggatcatgct ctccaggcgc
    11161 gcgcgcagca gccgcgcgag cacgggcgag gcgccgccgc tcgagaccgc gacgatgagc
    11221 ggcgcgcgct cgatgatcga gggcaggatg aacgagcaca gcgccggctg gtcgacgacg
    11281 ttcaccggaa tgagccgcgc cttggcggcg gcggccaccg agcggttcac cgcctggtcg
    11341 ctcgtggccg cgatcacggc ggcgtagccc tgcacgtgcg cgtcctcgaa gcgctcggcg
    11401 cggtgcgcaa tgcggcccgc cgcgcgctcg gcttccaggg ccttgcccag ctcgggggcg
    11461 agcaccgtca cgcgcgcacc cgcgcgcgcg agcagcgcgc acttgcgcgc ggcgacctcg
    11521 ccgccgccta ccaccaggca gggcgcgccg cgcaccttga gaaagatcgg caggaactcc
    11581 accgccgtat gatagattca atcggcaagg tgcatgcccc gatacctcac actgtcgcgc
    11641 gcggcgcgcc tggtcggcgt gcgccgcggc gtgctgcagt cgaagatccg caacggcgag
    11701 ctcgccgcct tcgagggcat ggtggcggag gcggacctcc tgcgcgccta cccggaggcg
    11761 cacctggagg acaacaccac cctggagcgg ctggaggcga tcaaggaggc cgccttcgcg
    11821 cggcgcgtgc gcgagcgcgt gctgccgagc gccgaagtgc tcatggcgcg cctgacggag
    11881 ctgggccgcg agcgcgctgc ggccgacgcc gagctcgcgc gcacgcgcgc gatcctcgcc
    11941 gagctcacgc agcggctgcg tgcctccgca gacgggacgc ggctcgccga ctgggtcgag
    12001 gcgcgcctcg cggcgccagc cccgggcggc ccggcgctgc tcgcgcacga aagcctgctt
    12061 aggatcatgt ccgcccacgt ccaggtgcag ccgagcggcc gcgagttctt cgtggacggc
    12121 aacgacagcc tgcttgaggc ggcgctgcgg gcggggctgt cgctcgacta cggctgcagc
    12181 gtcggcaact gcggcaagtg caaggcgcgc gtggtctcgg gccaggtgca ccgcctgcgg
    12241 cattcggact acgcgcttgg tgcggccgag aaggccgcgg gcgtggtgct catgtgctgc
    12301 cataccgcgg cgaccgacgt ggtcctcgag gcgcgcgaag cgcacggcgc ggccgacatg
    12361 ccgctgcaga cgatcgacac gcgcgtcaag gtggtgagcc cgatgggcga gcacatgcgg
    12421 ctgctgcacc tgcagacgcc acgcacgcag cggctgcgct tcctcgccgg gcagtcggtg
    12481 cggctctcgc tcggcgagga cgcggccgcg tccctgccgg tggcgagctg cccctgcgac
    12541 gagaggaatc tgcacttcca cgtgccccgg cgcgccggcg atgcgttcgc ggagcgcgtg
    12601 ttcgccgggc tgaagggcgt ggacacggtg cgcatcgagg ggccgcgcgg cgagttcgtg
    12661 ctcgacgagg actcgatccg cccgctcgtg ttcgtcgcct tcgacacggg ctttgcaccg
    12721 gtaaagagcc tcatcgagca cgccatggcg ctcgatgcgt cggagtcgct gcacctgtat
    12781 tggatcgcca cgggcgcggg cgggcactat ctggacaacc tgtgccgcgc gtggaacgac
    12841 gcgctggaca acttccgcta cgtgccgctc agcgcggccg gcggccgcgc cgacgaggcg
    12901 gcgctggggg ctctgctcaa ggaccatccg cggttcgacg agtgcgacgt ctatgtggcg
    12961 ggacccacgc cggccgcgca ggcggccgag ttcgcgctgc tcgagcgcgg cctgccgcgc
    13021 gcgcagctgc ggctgggcac cctcgagggc tgagccgcaa tgtcggaccg cgccagccgg
    13081 caagccgagc tcctcgccca ggccgggcgc acgctcgcgg gcggcggcct gggcctattc
    13141 gtgctgccgc cggagctcaa cctcgtggtc gagcgcggcc agggcagccg cgtgtgggat
    13201 gtcgcggggc gcgaatacat cgactaccac ctgggctcgg ggccggcgct gctgggacac
    13261 ggccatccgg cgatcagcgc ggcgatcgcc gcgcgcctgg acaagggcac gacctactac
    13321 tttctcaacg agcccgagat cgagctggcg cagcgcctga tcgacgcggt tgcctgcggc
    13381 gagcgggtgc actacgccgg gtcggggacc gaggccacct tctacgcgct gcgcatcgca
    13441 cgcgcccaca ccggccgcga caagatactc aagttcgagg gcgcctggca cggcatgcac
    13501 gactacggcc tctggggcac ggtgccgagc acgccgtcgg actatccgcg cgcgcgcccc
    13561 gattcggtgg gcgtgccgcg ccaggcgggc gagaccgtgc tggtcgcgcc ctttaacgac
    13621 gccgagcgcg cggtggcgct gatcgaggcg cacgcccgcg aactcgccgc ggtgatcgtc
    13681 gagcccctgc agcgcgtcct gcggcccgag ccgggctttc tcgaggcgct gcgcgcggtc
    13741 acgcagcggc acggcatcgt gctggtgttc gacgagatcg tcaccggctt tcgcatcgcc
    13801 tggggcggcg cgcaggagcg ctacggcgta gtgcccgatc tcgcctgcta cggcaaggcg
    13861 gtgagcggcg gactgccgat ggcgatgatc gtcgggcgcg ccgaggtcct cgccgtgctc
    13921 gatgcgcgca gccggccgcg cgagcaggtc gtctgggcga cgcatacgct gaacggcaat
    13981 ccgatttgtg cggcggccgg ctgcgcggcg ctcgacgtgc tggcgaagcc cggcgtctac
    14041 gaccacctgc accgcctcgg cgcgcgcctg cgcgccggca tcgtcgagtc cggcgcgcgc
    14101 cacggcttcg cggtgcaggc accgggcgag gacgcggtgt tcggcgtgcg cttcaccggg
    14161 cggcggccgc tgcgcaactg gatggatctc accaccgccg acaaggacct gggctggcgc
    14221 tgggcg
//
LOCUS       DBNHKE010000003         2266 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_519, whole genome shotgun sequence.
ACCESSION   DBNHKE010000003 DBNHKE010000000
VERSION     DBNHKE010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_519"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC16O9_00115"
     CDS             <1..321
                     /locus_tag="AC16O9_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515056.1"
                     /translation="AGWKRHDVKQYLWTNAHNTFGQVAFNHRYGKVYNRSLPVWYGRE
                     LDSRIPVVPSAENIHLFVIGGEAGRFSAFIPGWGHMSSPVLREIEGNAQSGGPRCVDG
                     TCYL"
     gene            338..877
                     /locus_tag="AC16O9_00120"
     CDS             338..877
                     /locus_tag="AC16O9_00120"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:617..619,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR6515057.1"
                     /translation="MIQVYDPRGVVGAEKKATAARIRALQGLRLGILDNTKWNANKLL
                     RELRDQLKAAHPLADVHYYRKESFSKFAAPELIEQIRRDNDIVVTAIGDUGSCTSCCM
                     HDGFSLESLGVPTAVIVTTEFLHEARVQREALGMQGLVPVVIQHPLSSLSDEEIRARA
                     AQALEQVIGVLRGTQPDPS"
     gene            874..2094
                     /locus_tag="AC16O9_00125"
     CDS             874..2094
                     /locus_tag="AC16O9_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6515058.1"
                     /translation="MSGALEGLVVLDLTRYLSGPHCTLLLAGLGAQVIKIDEPAVGDP
                     AALAPPYSGPSFERVSDADFGLSYLKRARGKQSATLNLKAPRGRDLFLRLAEHADVVV
                     ENFRPGVAERLGIGYATLAKRNPRLVYCALSGYGATGPDRDLKSYDLMAQAESGLMSL
                     TGAPDGPPMKTGGALSDMIAGAYAALGIVAALQERERSGLGQAVDVSMVDCLFSMMMD
                     EPLDAYETLGLAARQGNRIMRFSPFNAYRASDGWITLGVVTRDDWHGLLAAMGRDDLR
                     EHQDWSRVEWRIRNNAEVDKLVDEWAARFTVAEAIARLRAADVPCGAVRTAKEAIAWE
                     QLKARGMVQALRRPDGSPAGVAAAGMPLKFSRSTAHHDEPAPAPSTDTAEVLQRMLGL
                     GADEIARLRADGIA"
     gene            2104..2238
                     /locus_tag="AC16O9_00130"
     CDS             2104..2238
                     /locus_tag="AC16O9_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515059.1"
                     /translation="MAEKLNTGDVFPRLTLDLVDGGKLELPAGLDARYKVILFYRGHW
                     "
BASE COUNT          362 a          782 c          772 g          350 t
ORIGIN      
        1 gccggctgga agcgccacga cgtcaagcag tacctgtgga cgaacgccca caacacgttc
       61 ggccaggtcg cgttcaacca tcgctacggc aaggtctaca accgcagcct cccggtctgg
      121 tacgggcgcg agctcgactc gcgcatcccg gtggtgccca gcgcggagaa catccacttg
      181 ttcgtcatcg gcggcgaggc cgggcgcttc tcggccttca tcccgggctg ggggcacatg
      241 agctcgcccg tgctgcgtga aatagaaggc aacgcgcaga gcggcggccc gcgctgcgtc
      301 gacggcacct gctatctctg aggaaggagt ccatcgcatg atccaggtct acgacccgcg
      361 cggcgtggtc ggggccgaga agaaggccac cgcggcgcgc atccgggcgc tccagggcct
      421 gcggctcggc atcctcgaca acaccaagtg gaacgcgaac aagctgctgc gcgaactgcg
      481 cgatcagctg aaggccgcgc acccgctcgc cgatgtccac tactaccgca aggagagctt
      541 ctccaagttc gccgcgcccg agctgatcga gcagatccgg cgcgacaacg acatcgtcgt
      601 caccgccatc ggcgattgag gctcctgcac gtcgtgctgt atgcatgacg gcttcagtct
      661 cgaatcgctg ggggtgccga ccgcggtgat cgtcaccacg gagttcctgc acgaagcgcg
      721 ggtgcagcgc gaggcgctcg gcatgcaggg gctggtgccg gtggtgatcc agcatccgct
      781 ctcttcgctt tccgacgagg agatccgcgc ccgagccgcc caggcgctcg agcaggtgat
      841 cggcgtcttg cgcggcacgc agcccgatcc gtcatgagcg gcgcgctcga gggcctcgtg
      901 gtcctcgatc tcacgcgcta cctctcgggc ccgcactgca ccctgctcct cgcggggctc
      961 ggggcgcaag tgatcaagat cgacgagccc gccgtgggcg acccggcggc gctcgcgccg
     1021 ccgtattcgg gtccctcctt cgagcgcgtc tcggacgccg acttcggcct ctcctacctc
     1081 aagcgtgcgc ggggcaagca gtccgccacc ctcaatctga aggcgcctcg cgggcgcgac
     1141 ctgttcctgc gccttgcaga gcatgccgat gtcgtggtgg agaacttccg ccccggcgtc
     1201 gccgagcggc tcgggatcgg ctatgcgacg cttgcgaagc gcaatccgcg cctcgtctac
     1261 tgcgcgctct cgggctatgg cgctaccggc ccggaccgcg acctgaagtc ctacgacctc
     1321 atggcgcagg ccgaaagcgg cctcatgagc ctcaccggcg cgcccgacgg cccaccgatg
     1381 aagaccggcg gcgcgctctc cgacatgatc gccggcgcct acgccgcgct tggcatcgtc
     1441 gccgcgctcc aggagcgcga gcgctcgggc ctcgggcagg ccgtggacgt ctcgatggtg
     1501 gattgcctgt tctcgatgat gatggacgag ccgctcgatg cgtacgagac gctcggcctc
     1561 gcggcgcgcc agggcaaccg catcatgcgc ttttcgccgt tcaacgccta tcgcgcgagc
     1621 gacggctgga tcacgctcgg cgtggtgacg cgcgatgact ggcacggtct gctcgcggcg
     1681 atgggccgcg acgacctgcg cgagcaccag gactggagcc gcgtcgagtg gcgcatccgt
     1741 aacaacgccg aagtggacaa gctcgtggac gaatgggccg cgcgtttcac cgttgccgag
     1801 gcgatcgctc ggctgcgcgc ggcggacgtg ccctgcggcg ctgttcgcac tgcgaaagag
     1861 gcgatcgcgt gggagcagct caaggcgcgc ggcatggtgc aggcgctaag acggcctgac
     1921 gggtcgcctg cgggcgtcgc ggcggcgggc atgccgctca agttcagccg ctccacggcg
     1981 caccatgatg aaccggcacc ggcgccgagc acggacacgg cagaagtact gcagcgcatg
     2041 ctggggctgg gcgcagacga gatcgcgcgc ctcagggcgg acggaatcgc ctagaatcgg
     2101 ggcatggctg aaaaactgaa caccggcgac gtttttcccc gcctgacgct cgacctggtc
     2161 gacggcggaa agctcgagct gcccgcagga ctggacgcgc gctacaaggt catcctgttc
     2221 taccgcggtc actggtgacc gtactgtcgc cggcagttgg ccggct
//
LOCUS       DBNHKE010000004         3059 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_635426, whole genome shotgun sequence.
ACCESSION   DBNHKE010000004 DBNHKE010000000
VERSION     DBNHKE010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_635426"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="AC16O9_00135"
     CDS             complement(<1..692)
                     /locus_tag="AC16O9_00135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6515060.1"
                     /translation="MGRAELPLHAPGTQLDKGYIGQSVPRPNAQRLLQGRGAYTDDLR
                     FPRLAHVAFFRSPYAHARIRKLDFSKALKSPGVIGVFDGRAVAEYCTPWVAVLAHLKG
                     IKSPPQHAIAIERACWQGEACAAVVAESRSQAEDAVGLIEADWEELPAVIDMDESLAG
                     KTVIHPELGDNICFKREHDTGGVDEAFARADVVVEETYGFGRHTGVCNEGRAILADYN
                     AGEHSLTVYHAT"
     gene            complement(679..1152)
                     /locus_tag="AC16O9_00140"
     CDS             complement(679..1152)
                     /locus_tag="AC16O9_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6515061.1"
                     /translation="MEHAVEATITVNGTEVRRRVEPRQHLVDFLREELGLTGSHIGCE
                     HGVCGACTVRVDGVIVRGCLMLAVQADGCKVDTIEGLSDSGELSKLQKAFHDRNALQC
                     GFCTPGMLMAARDLQLRNPRATRDEIREHLSGNYCRCTGYQAIVDAIEEVLHGKG"
     gene            complement(1137..1940)
                     /locus_tag="AC16O9_00145"
     CDS             complement(1137..1940)
                     /locus_tag="AC16O9_00145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6515062.1"
                     /translation="MKAAPFSYAKPRALEEALELAARPNAKVLAGGQSLIASLNMRLS
                     SPDLLVDISGLKELKGISLERGVVRIGALATHAEIENSKVIQEHLPLFAAAVRHIAHP
                     AIRNRGTLGGSLALADPAAEYPACVLAMEGVIFARGAQGRRQIKAADFFKGLFETALE
                     PGELLTAVEITAQLKDERSAFLELARRQGDYATVGLAAFATNGTKRLAYFGAGDRPIL
                     ATHAAKAKDLESAKAALAKDLQPPADLYHSSATKLHLARVLLERAWNTL"
     gene            2001..>3059
                     /locus_tag="AC16O9_00150"
     CDS             2001..>3059
                     /locus_tag="AC16O9_00150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR6515063.1"
                     /translation="MAAAAMNIVLLPGDGIGPEIMAITEGALARLDRRFALGLRLSAH
                     EVGLAALAHSGSTLPDPVIQACRAADGFVLGPVGHHAYPPRAEGGRNPSGDLRIALDL
                     YANIRPSRSYEGLPHYGRTPMDLVIVRENTEGFYADRNMHQGSGEFMPTPDLALSVRK
                     VSAHASRRIAVTAFLLAKRRRRKVTAVHKANVLRLGEGLFLREVRAVAAQHPQVAYDE
                     QLVDSMAALLVRDAARYDVVVTTNMFGDILSDEAAELSGSLGLAGSINAGDAHCMAQA
                     QHGSAPDIAGTDRANPVSLLLSAAMLLGWLGERHAKKNFIEAQAALEAALARLLGDAA
                     TRTPDLGGALGTRAFGAAL"
BASE COUNT          415 a         1066 c         1102 g          476 t
ORIGIN      
        1 tgcgtcgcgt ggtacacggt gagcgaatgc tcgccggcgt tgtagtcggc gaggatcgcg
       61 cgcccttcgt tgcacacgcc ggtgtgccgc ccgaagccgt aggtttcctc cacgacgacg
      121 tcggccctgg cgaaggcctc gtcgacgccg cccgtgtcgt gctcgcgctt gaagcagatg
      181 ttgtcgccga gctcggggtg gatcacggtc ttgccggcga gcgactcgtc catgtcgatc
      241 accgccggca gctcctccca gtcggcctcg atcaggccca ccgcgtcctc ggcctggctg
      301 cgcgactcgg cgaccaccgc ggcgcaggcc tcgccctgcc agcaggcgcg ctcgatggcg
      361 atcgcgtgct gcggcgggct cttgatgcct ttcaggtgcg cgagcaccgc cacccagggc
      421 gtgcagtact cggccaccgc gcgcccgtcg aacacgccga tcacgccggg cgacttcagc
      481 gccttggaaa agtcgagctt cctgatcctc gcgtgcgcgt acgggctgcg gaagaacgcc
      541 acgtgcgcga ggcgcgggaa gcgaaggtcg tcggtgtagg cgccgcgccc ctggagcagg
      601 cgctgcgcgt tcgggcgcgg cacgctctgg ccgatatagc ccttgtcgag ctgcgtgccc
      661 ggggcgtgca gcggaagctc agcccttccc atgcagcacc tcctcgatgg cgtcgacgat
      721 cgcctggtag ccggtgcagc ggcagtaatt gcccgacagg tgctcgcgga tctcgtcgcg
      781 cgtggcgcgc gggttgcgca gctgcaggtc gcgcgccgcc atcagcatcc ccggcgtgca
      841 gaagccgcac tgcagcgcat tgcggtcgtg gaacgccttc tgcagcttcg acagctcgcc
      901 cgagtccgac agcccctcga tggtgtcgac cttgcagccg tcggcctgca ccgcgagcat
      961 caggcagccg cgcacgatca cgccgtcgac gcgcaccgtg caggcgccgc acacgccgtg
     1021 ctcgcagccg atgtgcgagc cggtgagccc gagctcctcg cgcaaaaaat ccaccaggtg
     1081 ctggcgcggc tcgacgcgcc ggcgcacctc ggtgccgttg acggtgatgg tggcttctac
     1141 agcgtgttcc atgcgcgctc caggagcacc cgtgccaggt gcagcttggt tgcagaggag
     1201 tggtagaggt ccgcgggcgg ctgcaggtcc ttcgccagcg cggccttggc ggattcgagg
     1261 tctttggctt tcgcggcgtg cgtggcgagg atcgggcggt cgccggcgcc gaagtaggcg
     1321 aggcgcttgg tgccgttggt ggcgaacgcg gcgagcccca cggtcgcgta gtcgccctgg
     1381 cgccgcgcga gctcgaggaa cgccgagcgc tcgtccttca gctgtgccgt gatctccacg
     1441 gcggtgagca gctcgcccgg ctcgagcgcg gtctcgaaca ggcccttgaa gaagtcggcg
     1501 gccttgatct ggcgccggcc ctgcgcgccc cgcgcgaaga tcacgccttc catggcgagc
     1561 acgcaggcgg ggtactcggc cgccgggtcg gcgagcgcaa gcgagccccc gagggtgccg
     1621 cggttgcgga tcgcggggtg cgcgatgtgg cgcaccgccg cggcaaacag cggcaggtgc
     1681 tcctggatca ccttggaatt ctcgatctcg gcgtgcgtgg cgagcgcgcc gatgcgcacc
     1741 acgccgcgct cgaggctgat gcccttgagc tccttcagcc cgctgatgtc gaccagcaga
     1801 tccggactgg agagccgcat gttgagcgag gcaatcaggc tctgcccgcc ggccagcacc
     1861 ttggcgttcg ggcgcgcggc gagctcgagc gcttcttcga gcgcgcgcgg cttcgcatag
     1921 gaaaacggcg cagccttcac cgggatcctc ctccttgggg ccgattatgc ccgaaccttt
     1981 gcgcgccgcg agccgctatg atggccgcgg cagcgatgaa catcgtgctc cttcccggcg
     2041 acggtatcgg cccggagatc atggcgatca cggagggggc gctcgcgcgc ctcgaccggc
     2101 gcttcgcgct cggcctgcgc ctgagcgcgc acgaggtggg gctcgccgcg ctggcgcatt
     2161 cgggcagcac gctgcccgac cccgtgatcc aggcctgccg cgcggccgac ggtttcgtgc
     2221 tcggcccggt ggggcaccac gcctatccgc cgcgcgccga gggcggacgc aatccttcgg
     2281 gcgacctgcg catcgcgctg gatctgtacg cgaacatccg gccgagccgc tcgtacgagg
     2341 gattgccgca ctacgggcgc acgcccatgg acctggtgat cgtgcgggag aacaccgaag
     2401 gcttctatgc cgaccgcaac atgcaccagg gctcgggcga attcatgccg acgccggatc
     2461 tggcgctgtc ggtgcgcaag gtgagcgcgc atgcgtcgcg gcgcatcgcc gtaacggcgt
     2521 tcttgctcgc gaagcgccgc cggcgcaagg tcactgcggt gcacaaggcc aacgtgctgc
     2581 gcttgggcga gggcctgttc ctgcgcgaag tgcgcgcggt ggcggcgcag catccgcagg
     2641 tggcgtacga cgagcagctc gtggactcga tggcggcgct cctggtgcgc gacgcggcgc
     2701 gctacgacgt cgtcgtcacg accaacatgt ttggcgacat cctctccgac gaggccgccg
     2761 agctctccgg cagcctcggc ctcgcgggct cgatcaacgc gggcgatgcg cattgcatgg
     2821 cgcaggcgca gcacggctcg gcgccggaca tcgccgggac ggaccgcgcc aatcccgtgt
     2881 cgctgctgct ctcggcggcg atgctgctgg gctggctcgg ggagcggcac gcgaagaaga
     2941 atttcatcga ggcgcaggcg gcgctggagg cagcgctcgc gcgcctgctc ggcgacgcgg
     3001 ctacgcgcac gccggacctg ggcggcgcgc tcggcacgcg cgcgttcggc gcggccctc
//
LOCUS       DBNHKE010000005        18281 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_53626, whole genome shotgun sequence.
ACCESSION   DBNHKE010000005 DBNHKE010000000
VERSION     DBNHKE010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 18281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18281
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_53626"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16O9_00155"
     CDS             <1..615
                     /locus_tag="AC16O9_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515064.1"
                     /translation="LLTGNWEGGARIKLSRFDLNRFFPFGAFGDGQEDRYGLPPLALE
                     RAAADILREAVVGFDTETRPAFRKGESHLPSLAQVATGRAVYLFPLQRVDCSGVLGAL
                     LGAPHVVAAGVALADDLRQLRQLFEFEVAAALDLGQVARRHGLKQTGLRNLTGIFLGT
                     RIPKGTKTTNWAAPRLSPQQVTYAATDAWACRELYLRFQGLGLL"
     gene            complement(607..984)
                     /locus_tag="AC16O9_00160"
     CDS             complement(607..984)
                     /locus_tag="AC16O9_00160"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0015038 - glutathione disulfide
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HIR6515065.1"
                     /translation="MGRPIFDEARIHPAIRERIAGWGVAIVKEVQQVVQSKDLVVVGM
                     KTNPAPRKARKLLDAAGIEYTYLEYGSYLSGWRRRLPLKLWTGWQTFPMIFVRGVLIG
                     GASDLEKLMASGALPQMLKKDSE"
     gene            complement(987..2558)
                     /locus_tag="AC16O9_00165"
     CDS             complement(987..2558)
                     /locus_tag="AC16O9_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6515066.1"
                     /translation="MTPAQLRARLLAREELALLDVREQGVHYKGHPFFACSLPLSRLE
                     LMVEELLPRRAVPLVLLDGGGEGLAERAATKLKALGYTDVDILQGGCAAWKATGGELF
                     SGVNVPSKAFGEFVEHHYGTPRIPPEELQRLVQAGKKLVILDSRPWDEYHRMNIPGGV
                     DVPGAELVYRTPDLTPDPGTLVVVNCAGRTRSIIGCQSLRNAGWTDVVALKDGTMGWE
                     LAGFECERGSARVAPAPSAEGRAKARAAADAVAKRFDVKFVGAPQVRAWLGDAARTTY
                     LLDVRTREEFDAGRLEGAHHAPGGQLVQATDEYVGVRNARIVLVDPERVRSVMTASWL
                     NQMGWDEVFVLEPGATDWPLERGPRASRVPGLQRAPEIDAAELARVRATVLDLSTSLK
                     FRARHLPGAWWAVRARLDAAREKLGEATALVLTSDDGLLAHLAAPEAAALWPRARVQV
                     LAGGNAAWFAAGLPAESGIGANATTTLDDVWYKPYDHEHGADYEKHARAYLDWEVALV
                     DQIKRDPAIRFRAYD"
     gene            complement(2555..3133)
                     /locus_tag="AC16O9_00170"
     CDS             complement(2555..3133)
                     /locus_tag="AC16O9_00170"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR6515067.1"
                     /translation="MDRTAERRKEIQAAVADIRDIERREGVNAASLEQIKSRLIRLAA
                     RTDLFDPKDYPPPKAGEKLRSCLYRLSEDPDHRYALYANASLGGYGTPAHNHTTWAVI
                     VGVTGEEQNRFYDRSPEGGVREKGRAVVRQGTGVAFMPEDLHSIHIDAPLLNFHMYGL
                     ALEQLHRREFYKEKEGRWDIFPAHSDIRDARA"
     gene            complement(3147..3545)
                     /gene="arfB"
                     /locus_tag="AC16O9_00175"
     CDS             complement(3147..3545)
                     /gene="arfB"
                     /locus_tag="AC16O9_00175"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR6515068.1"
                     /translation="MVIAIPEHEIDITAVRAQGAGGQNVNKVSSAVHLRFDVRASSLP
                     EDVKHRLLHRGDKRITRTGVVVIKAQQHRSLELNRAEAMARLQALVDAAATVPKRRRP
                     TRPTRASKERRIEAKQRRGRIKALRGRVRD"
     gene            complement(3539..5224)
                     /locus_tag="AC16O9_00180"
     CDS             complement(3539..5224)
                     /locus_tag="AC16O9_00180"
                     /inference="COORDINATES: protein motif:HMM:NF044955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HIR6515069.1"
                     /translation="MRLVLLALALAALPAGAQERVLEFHSTIRISDDGALTVTETIAV
                     QAEGREIRRGILRDFPTEYRGRFGEKVRVPFTVVRVTRNGNPEPWTTQALSNGVRVRI
                     GNAGLQLPHGRHVYEIMYRTSRQLGFFEGHDELYWNVNGNGWTFAMDWVSADVHLPAA
                     APAASLRLEAYTGAQGERRRDYVAVAREGGATFTSTRALPPHHGLTIVVGFPKGLVRE
                     PSTLERMWWWVDGNTGVLTGAAGLLALLAFLWWRWHLVGRDPRAGPAFPRYEAPPGLG
                     PAGVRLIDRMAYDPKCFAAGLLGLGARGFLRIRESGNAYVLERTGKAVEWLPGERILS
                     DLLPGPGKSVVIDKTHSPGVQLTLEKHQQELTALYDKTLFSRNRGSHAAGVGIGVVVV
                     VAMIIQETPTAALVAVIVPMIVLLALFARWLPAYSVQGRRLQDAIQGLRQYLGVAEKD
                     DLARMKAPPQTAEEFAKFLPYAVALGVERTWAERFTATLGLAAVTAAVAGYYSAESGG
                     MFHGGGIGDSIGGIAGTVSAAATPPGSSSGSASGGGGGGSSGGGGGGGGGSGW"
     gene            complement(5221..5742)
                     /locus_tag="AC16O9_00185"
     CDS             complement(5221..5742)
                     /locus_tag="AC16O9_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6515070.1"
                     /translation="MAAVALLAAGIWLYNRLIALRNLARQGFADIDVQLKRRADLVPQ
                     LVESVRGYASYEKALLVTVTELRGTALAAQDSAARFALERQLGERLQQLVVLQENYPQ
                     LKADANFRDLAAKLVEVEDHLQYARRFYNGAVQQYVTRLETFPDIVVARLFGFRSLPF
                     FETDDRQPAKVTL"
     gene            complement(5786..6055)
                     /gene="infA"
                     /locus_tag="AC16O9_00190"
     CDS             complement(5786..6055)
                     /gene="infA"
                     /locus_tag="AC16O9_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR6515071.1"
                     /translation="MAKEEMLEMEGVVQEVLPSTQFRVELTNGAQVLAYAGGKVRKNR
                     IRILAGDRVTMEMSPYDLTRGRISFRHKDENPVAMPQRRPMFRKR"
     gene            complement(6129..6899)
                     /locus_tag="AC16O9_00195"
     CDS             complement(6129..6899)
                     /locus_tag="AC16O9_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6515072.1"
                     /translation="MSWDPAQYLKFAGERMRPAVDLLARIPAAAPATVVDLGCGAGNL
                     APLLAARWPGAQLIGVDSSPEMLARARAAHPKAQFVQGDIGAWRPERPVDVLYSNAAL
                     HWLDGHERLFPALLAALAPGGWLAVQMPRNFEAPSHTSIVETIEQGPWRARLEPLVRR
                     EPVAAPGFYWRLLGRRAAELQVWETEYLQVLEGDNPVAEYTKGTWLKQFLDRLPGPER
                     AGFEADYRKRVLAWYPPEADGRTLFPFRRLFLVARRKP"
     gene            complement(6896..8113)
                     /locus_tag="AC16O9_00200"
     CDS             complement(6896..8113)
                     /locus_tag="AC16O9_00200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6515073.1"
                     /translation="MVPSELRAAVAPAAILAAAGLAVWIGPTLPRSLEGLRTLGPYAV
                     LVLGVAFALRFNRGRALILLASLLAAYAGYHYAQGLGGFAEKAVYTALVVLVPLNALI
                     AMTLPERGVAYHGSYRWLLLLGAEALLILWIAAAGRSALSGLAWQGVLEHWIFRSPPT
                     PLAGRLLFGAAFVTAVWRAWPKHTPLDLGLAAALVAFFIAAEWATSPAVFGAFLCAAG
                     AVVLVAFVQESHRLAFRDELTGLPGRRALEERLRALGPRYTLAMIDVDHFKKFNDTHG
                     HDIGDQVLKLVAARLAEVGGGGDAFRYGGEEFSVLFPGRGLKEALPFLERTRAAVEKY
                     RMAVRSEARPRQEEAGIRRRGDGAPTKLLSVTVSMGAAEPEVERQPPVQVLKAADEAL
                     YRAKKGGRNRVST"
     gene            complement(8103..10403)
                     /gene="parC"
                     /locus_tag="AC16O9_00205"
     CDS             complement(8103..10403)
                     /gene="parC"
                     /locus_tag="AC16O9_00205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR6515074.1"
                     /translation="MDTRTPDLFTPENGDDAAPIERHASQAYLGYAVSTVKARALPEI
                     ADGLKPVQRRILYAMGDAGGQGFAKCARYVGEVLGKYHPHGDSSTYEALVHLAQPFSM
                     RYPLIEGQGNFGSRDGDAAAAYRYTEARLSRYAELMLAEIGEGTVDFAKNYDGKFEEP
                     ALLPARLPFGLLNGSFGIPVGFSTRIPSHNLKEVAAAAAHVIRHPRAKLDELLEILPG
                     PDFPGGGQVISSAEEIRQAYETGRGSLLVKCKWETEQLARAQWRVVVTELPHGVSVRQ
                     VMEEIEALANPRPSLGKKEVSQEQKRLRQYLLDQIDQGGVRDESDRRSKLRLVIEPRS
                     SRQKPEELMAFLLARTSLETRTAVNLTWLGLDGLPETKGLAEILREWGDWRVQTVRRR
                     TEHRLEKCEARLHIVLGRLLAFVRIDEIIQLIRAADDQAEAKQKMMAKWKFSERQAED
                     IVNIRLGQLTKLDGVKLNDERKALEAERKDLKAILGNDKELKKLVVKELEADAKLYGD
                     GRRTLIKAEERAQVERTVVEEPLSVILSNKGWMRARTGHNLDLATLTFKDGDGLLQTL
                     ECKTTDAVILLAESGKTFTLEAAALPTGRGDGVPVNTLVHSSSDDIVWIGTGDPAQKL
                     LMNSSAGLGFVCKLGDLVSRTRQGKDFMTVGEGAQARPPALFSEGKVVAALSSDARLL
                     LFPLADVPVRPNGGVGVQLIALPEGVRLAAVAVGDGKSLQVSGVRRKNRAVETLDAAR
                     LAAHLGKRAQRGKVCEAGFRPDRIGS"
     gene            complement(10406..10624)
                     /locus_tag="AC16O9_00210"
     CDS             complement(10406..10624)
                     /locus_tag="AC16O9_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515075.1"
                     /translation="MPKKPRPFWERAYKGHALWLGKQKLGKVTLAAQGRYTWEAAGRA
                     GASDDLEKAKKAVELAVAMADKQLDLFD"
     gene            complement(10628..12589)
                     /locus_tag="AC16O9_00215"
     CDS             complement(10628..12589)
                     /locus_tag="AC16O9_00215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR6515076.1"
                     /translation="MAKQVYDASAIQALKGLEPVRRMPGMYTHTVHPLHIVQEAIDNA
                     VDEALAGHGKRISVRVFKDGSVEVEDEGRGIPVDMHPVEKRPAVEVAFGMLHAGGKFA
                     RSGEGVYHISGGLHGVGVAVSNALSRRLEVTVYRDGHKHTVAFEGGELKQKLKSERSK
                     ERRTGTVVRLWPDAKYFDSAAIPMAELEHLVRSKAYLLPGLTAVLEVEGKEKKSWKYE
                     RGMAQYFDFMLAGRELVAPLFSGERYFTATNIPDIEPGEGAAWAVGWVAEGDVFADSH
                     VNLIPTRSGGTHEAGFRAGIFDALASFMEARALTPKGVKLIADDLWARACFTLSARIV
                     RTQFHGQTKEKLTTRHAARLLELCVKDAFELWLNEHPDEGKRIAELAVEQAMARLAKG
                     KKVERKKSSGIATLPGKLVDCASGDVTRNELFLVEGDSAGGSAKEARDKEAQALLPLR
                     GKVLNTMSKDSRTVLANKELQAIATAIGVDPHGAEDGADLSGLRYGKVILMTDADVDG
                     GHIQALLLTFFFMHAPKLVEAGHIYVAQPPLYKVEVPAQGKGKPARRLYCLDDDELEE
                     ALAALKKEKVKEGSWEISRFKGLGEMSPEQLWETTMNPDTRRLVRMQLDAKQMRKVRQ
                     TFELLMDSGEAEGRRNWMREHWKTVEADV"
     gene            complement(12619..13068)
                     /locus_tag="AC16O9_00220"
     CDS             complement(12619..13068)
                     /locus_tag="AC16O9_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR6515077.1"
                     /translation="MALVLDCSIALAWFFADEKTAFTEAALDLVALEECWVPAVWRLE
                     FPNALLVAERRRRLTRDERLQVLDEATRLGLRVDGAVPELRAISDLAERHALSTYDAA
                     YLELAARQGATLITLDKELGAAAAAAGVAVHAPGRTSAAQMRGRYNT"
     gene            complement(13068..13322)
                     /locus_tag="AC16O9_00225"
     CDS             complement(13068..13322)
                     /locus_tag="AC16O9_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR6515078.1"
                     /translation="MTTVGSFEAKTKLAELLDKVEAGEVVTITRRGKAVARLVPARSE
                     EGAAQVRRVVEDIKRNRVGRGKPAGPGTSIAELIKAGRRY"
     gene            complement(13369..14436)
                     /locus_tag="AC16O9_00230"
     CDS             complement(13369..14436)
                     /locus_tag="AC16O9_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6515079.1"
                     /translation="MKLEHWSLHFYRLRYAREVVWANAREDAGLFALLRVEANGASGI
                     AEGTLKATWSGVSPASLKASLEDFLMPRLVGIELADETNVARALAGIPENRLAKGMIE
                     SACWTLRAAAAKQPLWKLWDAGRTRKLAWTVTRQKPAQMAADAAEVCARYGFRVLKVK
                     GGQGVDVDLRALAEIRAAVGDGVELYVDANGAYGRADAAEYLRALAAAGVTVAEDPCP
                     LQPDAGFEALQKASPIPILVDRSCASQQDAALYLERGAQALSTKPGRVGLAETRAIVA
                     QASPRGARTAVGLYAESALGTLVNLQQPGSMAAEQTFYLAMTEQVCAQVPAIRDGKIE
                     LPEAADLSQLVDWGRLQTLAL"
     gene            14473..15342
                     /locus_tag="AC16O9_00235"
     CDS             14473..15342
                     /locus_tag="AC16O9_00235"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6515080.1"
                     /translation="MASQAEVAVRGADQLGECPLWDEREAMLWWVDARFPAVKRLDPA
                     TGAVMMLVLPEVVGSLALRESGGMLAATKSGIHFLDPASGALEMKADPESHLPDNRFN
                     DGRCDRAGRFWAGTMSHVNRDPTGSLYRFEADFRSTRLRNAIIIPNSLAFSPDGRTMY
                     FADTNRHTIWAYDYDPASGAATGERVFADTGAGRPDGSCVDADGCLWNADYGASRVVR
                     YTPAGKVDGTYELPATNVTCCCFGGRDLDTLYITTATQRLSPQALAQQPLAGSLFALR
                     PGARGLPESRFAG"
     gene            complement(15347..16126)
                     /locus_tag="AC16O9_00240"
     CDS             complement(15347..16126)
                     /locus_tag="AC16O9_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HIR6515081.1"
                     /translation="MADPRAARRAIRSGAYARHTAGIAPGYVQGNLCILPREYAEDFQ
                     AFCERNPKPCPLLAVSAPGDPRLPALAADLDIRTDVPSYRVFRDGEFVEDRRDIRELW
                     RDDLVAFVLGCSFSFEEALIEAGVPVRHVAQGRNVPMYLTNVDTAPAGPFHGKLVVSM
                     RAFTPANAIRAIEITSRYPRVHGAPVHIGKPELIGIEDLNKPWVGDATEVRDDELPVF
                     WACGVTPQSVVRNARPPLCITHTPGHMVLTDLTNASLRSRD"
     gene            16192..17151
                     /locus_tag="AC16O9_00245"
     CDS             16192..17151
                     /locus_tag="AC16O9_00245"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6515082.1"
                     /translation="MPRHRDYLPHGVIPAVLLPFHEDFSIDEASYRAHLRDVGSVEGI
                     SAITVNAHASEVASCTFEEQRRVLEITQDEIGKRIPIVNGVYAEGSLEAARIAKMAQD
                     GGASALLVFPSGIFTFGQRPEMALEHFRRIADASDLPLIVFQYPLAGGQGYPLATLER
                     IMDAVPTVRAIKDWSANPQLHERQIRVLQSRRPTVNVLTTHSAWLMSSLALGCNGLLS
                     GSGAVIADLQAQLWRAVQAKDLARAQAINERIWPTVEAFYTEPWVDMHNRMKEALVLL
                     GKLPRAVVRPPLVKLAPAEIERIRQALVAAGLLAEGARAKKVA"
     gene            17154..>18281
                     /locus_tag="AC16O9_00250"
     CDS             17154..>18281
                     /locus_tag="AC16O9_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6515083.1"
                     /translation="MTQRLAAEVLVIGSGGAGMYAAIEAARLGRSVILLDRSLIGRGG
                     ATVMAQMTVAVALEEGGLDHPDHHYADTLAAGRGLCDPAMARLLCEEGPARIREMDAW
                     GVGWAREGGALKRAFAPGHDRARCVYVDFLNTGPAVSKTLRAQLNRVPEIRRVGDLLV
                     TALALRGGECVGATALHLASGEPVSIAAGATVLATGGLTRLYRRNSASANMMGDGYAL
                     ALAAGAELIDMEFVQFFPIGHLAPRLVGMDPIMWDPFRYKLGGRLLNGRREEFIHRYG
                     AKDDGRYVVTRDVATYAILKEAAAGRGSPHGGAWLSFEHCTEAELRAAGGPVIDRLAA
                     NGIDLTKQAIEVAPIAHYHMGGVRVDAKLATRVPGLFAAGEA"
BASE COUNT         2605 a         6610 c         6258 g         2808 t
ORIGIN      
        1 ctgctgaccg gcaactggga gggcggcgcc cgcatcaagc tctcgcgctt cgatctcaat
       61 cgctttttcc ccttcggcgc cttcggcgat ggtcaggagg accgttatgg tctcccgccg
      121 ctggcgctcg agcgcgccgc cgccgacatc ctgcgggaag cggtggtcgg cttcgacacc
      181 gagacgcgcc cggccttcag gaaaggcgag agccacctgc ccagcctcgc gcaagtggcg
      241 accgggcgcg cggtgtacct gtttccgctg cagcgtgtgg attgctccgg cgttctgggc
      301 gcgctcctcg gcgcgccaca cgtcgtcgcc gccggcgtgg cgctcgccga cgacttgcgc
      361 cagctgcggc agctgttcga gttcgaggtc gccgcggcgc tcgacctcgg ccaggtcgcg
      421 cggcgccacg gcctgaagca gacgggcctg cgcaacctca ccggcatctt tctcggcacg
      481 cgcatcccga agggcaccaa gaccaccaac tgggcggcgc cgcgcctgtc gccgcagcag
      541 gtgacgtacg cggcgaccga tgcctgggcc tgccgcgagc tctacctgcg tttccagggg
      601 ctcgggctac tctgagtcct tcttcagcat ctgcggcagc gcgccgctcg ccatcagctt
      661 ctcgaggtcg ctcgcgccgc cgatcagaac cccgcgcacg aagatcatgg gaaaggtctg
      721 ccagccggtc caaagcttga gcggcaggcg ccgccgccag ccggacaggt aggagccgta
      781 ttcgaggtag gtgtactcga tgcccgcggc gtcgaggagc ttgcgcgcct tccgcggcgc
      841 cgggttcgtc ttcatgccga cgacgacgag gtccttcgac tgcaccacct gctgcacttc
      901 cttcacgatg gccacgcccc agccggcgat cctctcccgg atcgccgggt ggatgcgcgc
      961 ttcatcgaag atcggccggc ccatggctaa tcgtacgccc ggaagcggat cgcggggtcg
     1021 cgcttgatct gatcgacgag cgccacctcc cagtcgaggt acgcgcgcgc gtgcttctcg
     1081 taatcggccc cgtgctcgtg gtcgtagggc ttgtaccaga cgtcgtcgag cgtggtggtc
     1141 gcattggcgc cgatgccgct ctcggccggc agcccggcgg caaaccacgc cgcattgcca
     1201 cccgcgagca cctgcacccg cgcgcgcggc cacagcgccg ctgcttcggg cgccgcgaga
     1261 tgcgcgagca gcccgtcgtc ggaggtcagc accagggcgg tcgcctcgcc gagcttttcg
     1321 cgtgccgcgt ccaggcgggc gcgcaccgcc caccaggcgc ccgggaggtg ccgggcacgg
     1381 aatttcaggc tggtcgaaag atcgagcacc gtggcgcgca cgcgcgccag ctcggccgca
     1441 tcgatctcgg gcgcgcgctg caggcccggc acgcgcgagg cgcgcggccc gcgctcgagc
     1501 ggccagtcgg tcgcaccggg ctcgagcacg aagacctcgt cccagcccat ctggtttagc
     1561 cacgaggcgg tcatcaccga gcgcacgcgc tccgggtcga cgagcacgat gcgcgcgttc
     1621 ctcacgccga cgtactcgtc ggtggcctgc acgagctgtc cgccgggtgc atggtgcgcg
     1681 ccctcgaggc ggccggcgtc gaactcctcg cgggtgcgca cgtcgagcag gtaggtggtg
     1741 cgcgcggcat ccccgagcca ggcgcgcacc tgcggcgcgc ctacgaattt cacgtcgaag
     1801 cgcttggcga cggcgtcagc cgccgcgcgc gccttcgccc gcccctcggc ggaaggcgcg
     1861 ggcgccacgc gcgccgagcc gcgttcgcac tcaaacccgg cgagctccca gcccatggtg
     1921 ccgtctttca gcgccaccac gtccgtccag cccgcgttcc tgagcgactg gcagccgatg
     1981 atgctgcgcg tgcgcccggc gcagttcacc accaccagcg tgccggggtc gggcgtcagg
     2041 tcgggcgtgc ggtagaccag ctccgcgccc ggcacgtcca cgcctcctgg aatgttcatg
     2101 cggtggtatt cgtcccaggg ccgcgaatcg aggatcacga gcttcttccc cgcctgcacc
     2161 aggcgctgca actcctcggg cgggatgcgc ggcgtgccgt aatgatgctc gacgaactcg
     2221 ccgaaggcct tcgagggcac gttcacgccg ctgaacagct ccccgccggt cgccttccag
     2281 gcggcgcagc cgccttgcag gatatcgacg tcggtgtacc cgagcgcttt cagcttcgtc
     2341 gccgcgcgct cggcaagccc ctcgccgccg ccgtccagga gcaccagcgg caccgcgcgg
     2401 cgcggcagca gctcctccac catgagctcc aggcgcgaca gcggcagtga gcatgcaaag
     2461 aacggatgcc ccttgtaatg cacgccctgc tcacgcacgt cgagcagcgc aagctcttcg
     2521 cgcgcaagca ggcgcgcgcg cagctgcgcg ggcgtcatgc gcgcgcgtcg cggatgtccg
     2581 agtgcgccgg gaagatgtcc cagcgccctt ccttttcctt gtagaactcg cgccggtgca
     2641 gctgctcaag cgcgaggccg tacatgtgga agttgagcag cggcgcgtcg atgtggatgg
     2701 aatgcagatc ctcgggcatg aaggcgacgc ccgtgccctg gcgcaccacg gcgcggccct
     2761 tctcgcgcac gccgccctcg gggctgcggt cgtaaaagcg gttctgctcc tcgccggtca
     2821 cgcccacgat caccgcccag gtggtgtggt tgtgcgccgg cgtgccgtag ccgccgagcg
     2881 aggcgttggc gtagagcgcg taacggtggt ccggatcctc ggacagccgg tacaggcagg
     2941 agcgcagctt ctcgcccgcc ttgggcggcg ggtagtcctt ggggtcgaac aggtcggtgc
     3001 gcgcggcgag tcggatgagg cgcgacttga tttgctcgag gctcgcggca ttcacccctt
     3061 cgcggcgctc gatgtcgcgg atgtcggcga cggcggcctg gatttccttg cggcgttcgg
     3121 cggtgcggtc catggccgaa atggtatcag tcgcgcacgc ggccgcgcag cgccttgatg
     3181 cgcccgcggc gctgcttggc ctcgatgcgg cgctccttcg aggcgcgcgt cggccgcgtc
     3241 ggccggcgcc gcttgggcac ggtggccgcg gcatccacca gcgcctgcag ccgcgccatg
     3301 gcctcggcgc ggttcagctc caggctgcgg tgctgctgtg ccttgatgac gacgacgccg
     3361 gtgcgcgtga tgcgcttgtc gccccggtgc agcagccggt gcttgacgtc ttccggcagc
     3421 gaagaggcgc gaacgtcgaa acgcaggtgc acggcgctcg agaccttgtt gacattctgt
     3481 ccgcctgcgc cctgcgcgcg caccgcggtg atgtcgatct cgtgctcggg gatggcgatc
     3541 accagccgct gcctccgccg cccccgccgc cgccgcccga ggagccgccg ccgcccccgc
     3601 ccgaggcgct gcccgaactc gagcccggcg gcgtcgccgc cgcgctgacg gtgccggcga
     3661 tgccgccgat cgaatcgccg attccgccgc cgtggaacat gccgccggac tcggcactgt
     3721 agtagccggc gaccgccgcc gtcactgcgg caaggccgag ggtcgcggtg aagcgctcgg
     3781 cccaggttcg ctcgacgccg agcgccaccg cgtacggcag gaacttcgcg aactcctccg
     3841 cagtctgcgg cggcgccttc atgcgtgcga ggtcgtcctt ctcggccacg cccaggtact
     3901 ggcgcaggcc ctggatcgcg tcctgcagcc tgcggccctg cacgctgtag gcgggcagcc
     3961 agcgcgcgaa cagcgcgagc agtacgatca tcggcacgat gacggccacc agcgccgccg
     4021 tgggcgtctc ctggatgatc atggccacca ccacgacgac gccaatgcct acgccggctg
     4081 cgtgcgagcc gcgattgcgc gagaacagcg tcttgtcgta gagcgccgtc agctcctgct
     4141 ggtgcttttc cagcgtgagc tgcacgcccg ggctgtgcgt cttgtcgatg acgacggact
     4201 tgcccggccc gggcagcagg tccgacagga tgcgctcgcc cggcagccat tccaccgcct
     4261 tgccggtgcg ctccagcaca taggcgttgc ccgattcgcg tatacgcagg aagccgcgcg
     4321 cccccagccc gagcagcccc gcggcgaagc acttcgggtc gtacgccatc cgatcgatca
     4381 agcgcacccc cgcaggcccg agccccggcg gcgcttcgta gcgcgggaac gcgggaccgg
     4441 cgcgcggatc gcgcccgacg aggtgccagc gccaccagag aaaagcgagc agcgccagca
     4501 ggcccgccgc gccggtgagt acgcccgtgt tgccgtccac ccaccaccac atccgctcca
     4561 gggtggaggg ctcgcgcacc aggcctttgg gaaaccccac cacgatggtc aggccgtgat
     4621 gcggcggcag cgcgcgcgtg ctggtgaagg tggcgccgcc ttcgcgcgcc accgccacgt
     4681 agtcgcgccg gcgttcgccc tgcgcgcccg tatacgcctc caggcgcaag ctcgccgccg
     4741 gcgctgccgc gggcaggtgg acgtcggcgg aaacccagtc catggcgaag gtccagccgt
     4801 tgccgttcac gttccagtac agctcgtcgt ggccctcgaa aaaaccgagc tggcgcgacg
     4861 tgcggtacat gatctcgtag acgtgccgtc cgtgcggcag ctgcaggccg gcattgccga
     4921 tgcgcacgcg cacgccattg ctcagcgctt gcgtggtcca gggctcgggg ttgccgttgc
     4981 gcgtcacccg cacgaccgtg aacggcacgc gcaccttctc gccgaagcgt ccgcggtact
     5041 cggtgggaaa gtcgcgcagg atgccgcgcc tgatctcgcg gccctcagcc tgcacggcga
     5101 tggtctcggt caccgtcagc gcgccgtcgt cgctgatgcg gatggtgctg tggaactcga
     5161 gcacccgctc ctgcgcgccg gccggcagcg cggcgagagc cagcgcgaga agcaccagcc
     5221 tcacagcgtc actttcgccg gctggcgatc gtcggtctcg aaaaagggca gcgaccggaa
     5281 accgaacagg cgcgccacga cgatgtcggg aaaagtctcc aggcgcgtca cgtattgctg
     5341 taccgcgccg ttgtagaagc gccgcgcgta ctgcaggtgg tcctcgacct cgaccagctt
     5401 cgccgcgagg tcgcggaagt tggcgtcggc cttcagctgc gggtagttct cctgcagaac
     5461 gacgagctgc tgcagccgct cgccgagctg gcgctcgagc gcgaagcggg ccgcgctgtc
     5521 ctgcgcggcg agcgcggtgc cgcgcagctc ggtcacggtg acgaggagcg ccttttcgta
     5581 gctcgcgtag ccgcgcaccg actccacgag ctgcggcacg aggtcggcgc ggcgcttgag
     5641 ctgcacgtcg atgtcggcga agccctggcg cgcaaggttg cgcagggcga tgaggcggtt
     5701 gtaaagccag atgccggcgg cgaggagggc gaccgccgcc aaaagcgcga tccacattgc
     5761 agtggcgcgc cggacctggt gcccgctagc gcttgcggaa catcggccgc cgctgcggca
     5821 tggcgaccgg gttttcgtcc ttgtggcgga agctgatgcg accccgcgtc agatcgtagg
     5881 gggacatttc catcgtcacg cgatcgcccg cgaggatgcg gatgcgattc ttgcgtacct
     5941 tgccgcctgc gtaggcgagg acctgcgcgc cgttggtgag ctcgacgcga aactgcgtgc
     6001 tgggcagcac ttcctgcacc accccttcca tctccagcat ttcttccttg gccataactc
     6061 cgatccggtt attgcgaggc gggcagtatg ctccctaacg cccgggcgaa caaggcgatt
     6121 ctggaactct agggcttgcg ccgcgcgacc aggaacagcc gccggaacgg aaacagcgtg
     6181 cgcccgtcgg cttccggcgg ataccaggcg agcacgcgct tgcggtagtc ggcttcgaac
     6241 cccgcgcgct cgggccccgg caagcggtcg aggaactgct tgagccaggt gcccttggtg
     6301 tactcggcta ccgggttgtc gccctcgagc acctgcaggt actcggtttc ccagacctgc
     6361 agctcggccg cgcggcgccc gagcagccgc cagtaaaagc ccggcgcggc caccggctcg
     6421 cgccgcacga gcggctcgag ccgcgcgcgc cacggtccct gctcgatggt ctcgacgatg
     6481 ctcgtatgcg agggcgcctc gaagttgcgc ggcatctgca ccgcgagcca gccgccgggc
     6541 gccagcgccg cgagcagcgc cggaaacagc cgctcgtggc cgtcgagcca gtgcagcgcc
     6601 gcgttgctgt agagcacgtc gacgggccgc tcggggcgcc aggcgccgat gtcgccctgc
     6661 acgaactgcg ccttggggtg cgccgcgcgg gcgcgcgcaa gcatctcggg cgagctgtcc
     6721 acgccgatga gctgcgcgcc cggccagcgc gccgcaagga gcggcgcaag gttgcccgcg
     6781 ccgcagccca ggtcgacgac cgttgccggc gccgcggccg gtatgcgcgc cagcaagtcc
     6841 accgccggcc gcatccgttc gccggcgaat ttcaggtact gggcgggatc ccagctcacg
     6901 tgctcacgcg gttgcggccg cccttcttcg cgcgatagag cgcctcgtcg gccgctttca
     6961 gcacctgcac cggcggctga cgctcgactt cgggctcggc ggcgcccatc gacaccgtga
     7021 ccgagagcag cttggtcggc gcgccgtcgc cgcgccggcg gatgcccgcc tcctcctggc
     7081 gcgggcgcgc ctcgctgcgc accgccatgc ggtatttctc cacggccgcg cgcgtgcgct
     7141 caaggaacgg cagcgcttcc ttcaggccgc ggcccgggaa cagcacgctg aattcctcgc
     7201 cgccgtagcg aaacgcgtcg ccgccgccgc ccacctcggc gaggcgcgcc gcgaccagct
     7261 tcagcacctg gtcgccgatg tcgtggccgt gcgtgtcgtt gaacttcttg aagtgatcga
     7321 cgtcgatcat ggcgagcgtg tagcgcgggc cgagcgcgcg caaccgctct tccagcgcgc
     7381 gccgcccggg cagacccgtc agctcgtcgc ggaacgccag gcgatgcgac tcctgcacga
     7441 aggcgaccag cacgaccgcg cccgcagcgc acaggaaagc gccgaacacc gcgggcgacg
     7501 tggcccattc ggcggcgatg aagaacgcga ccagcgcggc ggcgagcccc aagtcgagcg
     7561 gcgtgtgctt gggccacgcg cgccacaccg cggtgacgaa cgccgcgccg aacaggagcc
     7621 ggccggcaag cggcgtcggc ggcgagcgga agatccagtg ctcgagcacg ccctgccagg
     7681 cgagcccgga gagcgcgctg cggccggccg cggcgatcca caggatgagc agcgcctcgg
     7741 cgccgagcag cagcagccag cggtagctgc cgtgataggc gacgccgcgc tcgggcagcg
     7801 tcatggcgat gagcgcgttg agcggcacca gcacgacgag cgccgtgtac acggccttct
     7861 ccgcgaagcc tcccagcccc tgcgcgtagt ggtagccggc gtaggcggcc aggagcgaag
     7921 cgagcaggat gagcgcgcgg ccgcgattga agcgcagcgc gaaggcgacc ccgagcacga
     7981 gcaccgcgta gggtccgagc gtgcgcagtc cctcgagcga gcggggcagg gtcgggccga
     8041 tccacaccgc gagtcccgcg gcggcgagga tcgccgccgg cgccaccgcg gcgcgcagtt
     8101 cgctaggaac caatgcgatc cggcctgaag cccgcctcgc agaccttgcc gcgctgcgcg
     8161 cgcttgccca ggtgcgccgc gagccgcgcg gcgtcgagcg tttccacggc gcggttcttg
     8221 cgacgcacgc cgctcacctg cagcgacttg ccgtcgccga cggcgactgc ggcgagcctc
     8281 acgccctcgg gcagcgcgat caactgcacg ccgacgccgc cgttcggccg caccggcacg
     8341 tccgcgagcg gaaagagcag cagccgcgca tccgaagaga gcgccgccac gaccttgccc
     8401 tcgctgaaca gcgcgggcgg ccgcgcctgg gcgccctcgc ccacggtcat gaagtccttg
     8461 ccctggcgcg tgcgcgagac gaggtcgccc agcttgcaca cgaagcccag gccggcgctc
     8521 gaattcatca gcagcttctg cgcggggtcg cccgtgccga tccacacgat gtcgtcggaa
     8581 gacgagtgca ccagcgtgtt caccggcacg ccgtcgccgc gcccggtagg cagcgccgcc
     8641 gcctccagcg tgaaggtctt gcccgactcc gcgagcagga tcaccgcgtc ggtagtcttg
     8701 cactcgagcg tctgcagcaa gccgtcgcca tccttgaagg tgagcgtggc gagatcgagg
     8761 ttgtggccgg tgcgcgcgcg catccagccc ttgttcgaca ggatgacgct gagcggctcc
     8821 tcgaccaccg tgcgctcgac ctgcgcgcgc tcctcggcct tgatgagcgt gcgccgcccg
     8881 tcgccgtaga gcttggcgtc ggcctccagc tccttcacca cgagcttctt cagctccttg
     8941 tcgttgccca ggatcgcctt caggtccttc ctctcggcct cgagcgcctt tctctcgtcg
     9001 ttcagcttca cgccgtcgag cttggtgagc tgcccgaggc ggatgttgac gatgtcctcg
     9061 gcctggcgct ccgagaactt ccatttcgcc atcatctttt gcttcgcctc ggcctgatcg
     9121 tccgcggcgc ggatcagctg gatgatctcg tcgatgcgca cgaaggcgag cagccgcccc
     9181 agcacgatgt gcagccgcgc ctcgcacttt tccagccggt gctcggtgcg ccggcgcacc
     9241 gtctgcacgc gccagtcgcc ccactcgcgc aggatctcgg cgagcccctt ggtctcgggc
     9301 agtccgtcga ggccgagcca ggtgaggttc accgcggtgc gcgtctcgag cgacgtgcgc
     9361 gccagcagga acgccatcag ctcctccggc ttctgccgcg aggagcgcgg ctcgatcacc
     9421 agccgcagct tcgagcgccg gtcgctctcg tcgcgcaccc cgccctggtc gatctggtcg
     9481 agcaggtact gccgcaggcg cttctgttcc tgggagactt ccttcttgcc caggctcggc
     9541 cgcggattcg ccagcgcctc gatctcctcc atcacctgtc tgacggatac gccgtgcggc
     9601 agctccgtga ccacgacgcg ccactgcgcg cgcgccagct gctcggtttc ccacttgcac
     9661 ttgaccagca gcgagccgcg cccggtctcg taggcctggc ggatctcctc ggcgctcgag
     9721 atcacctggc cgccgccggg gaaatccgga ccgggcagga tctcgagcag ctcgtcgagc
     9781 ttggcgcgcg gatgccggat gacgtgtgcc gccgcggccg cgacctcctt caggttgtgc
     9841 gatgggatgc gcgtggaaaa gcccaccggg atgccgaacg agccgttcag caggccgaag
     9901 ggcagccgcg cgggcagcag cgccggctcc tcgaacttgc cgtcgtagtt cttcgcgaag
     9961 tcgaccgtgc cctcgccgat ctcggcgagc atcagctccg cgtagcgcga cagcctggcc
    10021 tcggtgtagc ggtaggcggc ggcggcgtcg ccgtcgcgcg agccgaagtt gccctgaccc
    10081 tcgatcagcg ggtagcgcat cgagaacggc tgcgccaggt gcaccagcgc ctcgtaggtc
    10141 gaggaatcgc cgtgcgggtg gtacttgccg agcacctcgc cgacgtagcg cgcgcacttg
    10201 gcgaagccct gtccgcccgc gtcacccatc gcatagagga tgcgccgctg taccggcttc
    10261 aggccgtcgg cgatctcggg aagcgcccgc gccttgaccg tggacacggc gtagccgagg
    10321 taggcttgcg aggcgtggcg ctcgatcggc gccgcgtcgt cgccgttctc gggagtaaac
    10381 aggtcgggcg tgcgggtgtc catgctcagt cgaatagatc cagttgcttg tcggccatcg
    10441 cgacggcgag ctccaccgcc tttttcgcct tctccaggtc atcgctcgcg cccgcccggc
    10501 ctgcggcctc ccaggtatag cggccctggg cggcgagcgt caccttgccg agcttctgct
    10561 tgccaagcca gagcgcgtgg cccttgtagg cgcgctccca gaacggccgc ggcttcttcg
    10621 gcatgcgcta gacgtccgct tccaccgtct tccagtgctc gcgcatccag ttgcgcctgc
    10681 cttcggcctc gcccgagtcc atcagcagct cgaaggtctg gcgcaccttc cgcatctgct
    10741 tggcgtcgag ctgcatgcgc accaggcgcc gcgtatccgg gttcatcgtc gtctcccaga
    10801 gctgctcggg gctcatctcg cccaggccct tgaagcgcga aatctcccag ctgccttcct
    10861 tcaccttctc tttcttcagc gccgcgagcg cttcctccag ctcgtcgtcg tcgaggcagt
    10921 acaggcgccg cgccggcttg cccttgccct gcgccggcac ctcgaccttg tagagcggcg
    10981 gctgcgccac gtagatgtgg cccgcctcca ccagcttggg cgcgtgcatg aagaagaagg
    11041 tgagcaggag cgcctggatg tgcccgccgt cgacgtcggc gtcggtcatc aggatcacct
    11101 tgccgtagcg cagccccgag aggtccgctc cgtcctcggc gccgtgcgga tcgacgccga
    11161 tcgccgtggc gatcgcctgc agctccttgt tggcgagcac ggtgcgcgag tccttcgaca
    11221 tggtgttcag caccttgccc cggagcggca gcagcgcctg cgcttccttg tcccgcgctt
    11281 ccttggccga gccgccggcc gagtcgccct cgacgaggaa cagttcgttc cgcgtcacgt
    11341 cgcccgaggc gcagtcgacg agctttccgg gcagcgtggc gatgccggag gacttcttcc
    11401 tctcgacctt cttgcccttg gcgaggcgcg ccatcgcctg ctcgacggcg agctcggcga
    11461 tccgcttgcc ctcgtcggga tgctcgttca gccagagctc gaacgcgtcc ttcacgcaca
    11521 gctcgagcag gcgcgccgcg tgccgcgtgg tgagcttttc cttggtctgg ccgtggaact
    11581 gcgtgcgcac gatccgcgcc gagagcgtga agcaggcgcg cgcccagagg tcgtcggcga
    11641 tcagcttcac gcccttcggc gtcagcgcgc gcgcctccat gaaggaggcc agcgcgtcga
    11701 agatgccggc gcgaaagccc gcctcgtgcg tgccgcccga gcgcgtcggg atcaggttga
    11761 cgtggctgtc ggcgaacacg tcgccctcgg ccacccagcc caccgcccag gccgcgcctt
    11821 cgccgggctc gatgtcggga atgttggtgg cggtgaaata gcgctcgccg gaaaaaagcg
    11881 gcgccaccag ctcgcgcccg gcaagcatga agtcgaaata ctgcgccatg ccgcgctcgt
    11941 acttccagct cttcttctcc ttgccctcga cctcgagcac ggcggtgagg ccgggcagca
    12001 ggtaggcctt ggagcgcacc aggtgctcga gctcggccat cgggatcgcc gccgagtcga
    12061 agtacttcgc gtcgggccac aggcgcacca cggtaccggt gcgccgctcc ttcgatcgct
    12121 ccgacttcag cttctgcttg agctcgccgc cctcgaaggc gaccgtgtgc ttgtgtccgt
    12181 cgcggtagac cgtgacctcg aggcgcctgg acagcgcgtt ggagaccgcc acgccgacac
    12241 cgtgcaggcc gcccgagatg tggtacacgc cctcgcccga gcgcgcgaac ttgccgcccg
    12301 cgtgcagcat gccgaacgcg acctcgaccg ccgggcgctt ctccaccggg tgcatgtcca
    12361 ccgggatgcc gcggccctcg tcctcgacct cgaccgagcc gtccttgaac acgcgcacgc
    12421 tgattctctt gccgtggccg gcgagcgcct cgtcgaccgc gttgtcgatc gcctcctgca
    12481 cgatgtgcag cgggtgcacg gtgtgcgtgt acatgcccgg catgcggcgc acgggctcga
    12541 ggcccttcag ggcctggatg gcggaggcgt cgtagacctg ctttgccatt tatcttgtgg
    12601 tttccggttc gttcgctact aggtattgta tcgcccgcgc atttgcgctg ccgaggtacg
    12661 ccccggcgcg tgcaccgcga cgcctgccgc ggccgccgcg gcgccgagct ccttgtccag
    12721 cgtgatcagg gtcgcgcctt ggcgcgccgc gagctccagg tacgccgcgt cgtaggtgct
    12781 gagcgcatgg cgttcggcga gatcgctgat ggcgcgcaac tcgggcacgg cgccgtccac
    12841 gcgcaaaccg aggcgcgtcg cctcgtccag cacttgcagc cgctcgtcgc gcgtcaggcg
    12901 ccgccggcgc tcggcgacca gcagcgcgtt cgggaactcc aggcgccata cggcgggcac
    12961 ccagcattcc tcgagcgcga ccagatcgag cgccgcctcg gtgaacgcgg ttttttcgtc
    13021 ggcgaagaac caggcgagcg cgatcgagca gtcgagcacc agcgccatca gtagcggcgc
    13081 ccggccttga tcagctcggc gatcgacgtg ccgggcccgg cgggcttgcc gcggccgacg
    13141 cgattgcgct tgatgtcctc caccacgcgg cgcacctgcg cggcgccttc ttcggagcgg
    13201 gcgggcacga ggcgcgccac cgccttgccg cgccgggtga tggtgaccac ctcccccgcc
    13261 tcgaccttgt cgaggagttc ggcgagcttg gtcttcgctt cgaaagagcc gacggtggtc
    13321 atgccttatc caactagaca accagtcggg aggttaccac ccggtctgtc acaaggcaag
    13381 cgtctgcaag cgcccccaat ccacgagttg cgacagatcc gcggcctccg gcagctcgat
    13441 cttcccgtcc cggattgccg gtacctgcgc gcaaacctgc tcggtcatcg ccagataaaa
    13501 ggtttgctcg gccgccatgc tccccggctg ctgcaggttg accagcgtgc cgagcgcgct
    13561 ttcggcgtac agccccaccg cggtgcgcgc gccgcgcggc gacgcctgcg cgacgatggc
    13621 gcgcgtctcg gccaacccca cccttccggg cttggtcgag agcgcctgcg cgccccgctc
    13681 caggtacagc gccgcgtctt gctgcgacgc gcagctgcga tcgacgagga tcgggatcgg
    13741 gcttgccttc tgcagggcct cgaagccggc atcgggctgc aggggacagg gatcctccgc
    13801 caccgtcacg cccgccgcgg ccagcgcccg caggtattcg gccgcgtcgg cacgcccgta
    13861 cgcaccattg gcgtcgacgt agagctccac gccatcgccg accgcggcgc ggatttccgc
    13921 cagcgcccgc aggtccacgt ccacgccctg cccgcctttc accttcaaga cgcgaaaacc
    13981 ataccgcgcg cacacctcgg ccgcgtccgc cgccatttgc gcgggcttct gccgcgtcac
    14041 ggtccaggcc agcttgcgcg tacgacccgc gtcccacagc ttccataatg gctgcttcgc
    14101 ggccgcggcg cgcagcgtcc agcaggcgct ctcgatcatg cccttcgcga ggcggttttc
    14161 cgggataccg gcgagggccc gtgccacgtt cgtttcgtcg gcaagctcga tccccacgag
    14221 gcgcggcatg aggaaatcct caagcgacgc cttgagcgaa gccggcgata cgccgctcca
    14281 ggtggccttg agcgtgcctt cggcgatgcc gctcgcgccg ttcgcctcga cgcgcagaag
    14341 cgcgaacagg cccgcatcct cgcgcgcgtt cgcccacacc acttcacgcg cgtagcgcaa
    14401 gcggtagaag tgcagcgacc agtgctcgag cttcacggcg tgctatcgtg cccgcgcgag
    14461 gggattcggg caatggcgtc tcaggcggag gtcgcggtgc gcggcgcgga ccagctcggc
    14521 gaatgcccgc tgtgggacga gcgcgaagcg atgctgtggt gggtcgacgc gcgctttccg
    14581 gcggtcaagc gcctcgaccc cgccaccggc gcggtgatga tgctggtgct gccggaggtc
    14641 gtgggcagcc ttgcgctgcg cgagtcgggc ggcatgctgg cggcgacgaa aagcggcatc
    14701 cactttctcg atccggcgag cggcgcgctc gagatgaaag ccgatccgga atcgcacctg
    14761 cccgataacc gcttcaacga cggccgctgc gaccgcgccg ggcgcttctg ggccggcacc
    14821 atgagccacg tgaatcgcga cccgaccgga tcgctgtacc gcttcgaggc ggatttccgc
    14881 agcacccggc tgcgcaacgc gatcatcatc ccgaacagcc tcgccttcag ccccgacggg
    14941 cgcacgatgt acttcgccga taccaaccgc cacacgatct gggcctacga ctatgacccg
    15001 gcaagcggcg cagcgacggg cgagcgcgtg ttcgccgaca ccggggcggg acggcccgac
    15061 ggctcctgcg tcgacgccga cggctgcctg tggaacgcgg attacggcgc gtcgcgcgtc
    15121 gtgcgttaca cgccggcggg aaaggtggac ggcacctacg agctgccggc gaccaacgtc
    15181 acctgctgct gcttcggcgg cagggacctg gacacgctgt acatcacgac ggcgacgcag
    15241 cgcctttcgc cgcaggctct ggcgcagcag ccgctggccg gcagcctgtt cgcgctgcgg
    15301 ccgggcgcgc gcggcctgcc ggaatcgcgc ttcgcgggct aaagcgctag tcgcgcgagc
    15361 gcaggctcgc gttcgtcagg tcggtgagca ccatgtgccc gggcgtatgc gtgatgcaca
    15421 gcggcgggcg cgcgttgcgc accaccgact gcggcgtcac cccgcaggcc cagaagaccg
    15481 gcagctcgtc gtcgcgcacc tcggtggcgt cgcccaccca cggcttgttc aggtcctcga
    15541 tgccgatcag ctcgggcttg ccgatgtgca ccggcgcgcc gtgcacgcgg ggatagcgcg
    15601 aggtgatctc gatggcgcgg atcgcgttgg cgggcgtgaa cgcgcgcatc gacaccacca
    15661 gcttgccgtg gaacggcccc gccggcgcgg tatcgacatt ggtgaggtac atcggcacgt
    15721 tcctgccctg cgcgacgtgg cgcacgggta cgcccgcctc gatcagcgcc tcctcgaacg
    15781 agaacgagca gccgagcacg aacgccacca ggtcgtcgcg ccacagctcg cggatgtcgc
    15841 gccggtcctc gacgaactcg ccgtcgcgaa acacgcgata gctcggtacg tcagtgcgga
    15901 tgtcgaggtc ggcggcgagc gccggcagtc tcgggtcgcc cggcgccgag accgcgagca
    15961 gcgggcaggg cttggggttg cgctcgcaga aggcctggaa gtcctcggcg tactcgcgcg
    16021 gcaggatgca caggttcccc tgcacgtagc cgggcgcgat gcccgcggtg tggcgggcgt
    16081 acgcgccgct gcggatcgcg cggcgcgccg cgcgcgggtc ggccatcgga gaggcagcca
    16141 tgggcgacaa gggtacactg gagtccggcc acgcagtcga tggagcgacc catgccgaga
    16201 catcgcgatt accttcccca cggcgtcatc cccgcggtgc tgctgccgtt ccacgaggat
    16261 ttctcgatcg acgaggcgtc ctaccgcgcg cacctgaggg acgtcggctc ggtcgaaggc
    16321 atctcggcga tcacggtcaa cgcgcacgcc tcggaggtcg cctcatgcac cttcgaggag
    16381 cagcggcgcg tgctcgagat cacccaggac gagatcggca agcgcatccc gatcgtgaac
    16441 ggggtctacg ccgagggcag cctggaagcg gcgcgcatcg cgaagatggc gcaggacggc
    16501 ggcgcctcgg cgctgctcgt ttttccctcc ggcatcttca ccttcggcca gcgcccggag
    16561 atggcgctgg agcatttccg gcggattgcc gacgcgagcg acctgccgct catcgtcttc
    16621 cagtacccgc tcgcgggcgg ccagggatat ccgctcgcca cgctggagag aatcatggac
    16681 gcggtgccca cggtgcgcgc gatcaaggac tggagcgcca acccgcagtt gcacgagcgc
    16741 cagatccgcg tgctgcaatc gcgcaggccc acggtaaacg tcctcaccac gcacagcgcg
    16801 tggctgatga gctcgctcgc gctgggctgc aacggacttc tctcgggcag cggcgcggtg
    16861 atcgccgacc tgcaggcaca gctgtggcgc gcggtacagg ccaaggacct cgccagggcg
    16921 caagccatca acgagcgcat ctggcccacg gtcgaagcgt tctacaccga gccctgggtc
    16981 gacatgcaca accgcatgaa ggaggcgctg gtgctgctcg gcaagctgcc gcgcgcggtg
    17041 gtgcgaccgc cgctcgtcaa gctcgcgccc gccgagatcg agcgcatccg ccaggcgctc
    17101 gtcgccgccg ggctcctcgc cgaaggggcg cgcgccaaga aagtcgcctg acgatgacgc
    17161 agcgcctcgc cgccgaggtg ctggtcatcg gctccggcgg cgcgggcatg tacgccgcga
    17221 tcgaggcggc gcgcctcggg cgctcggtga tcctgcttga tcgaagcctg atcggccgcg
    17281 gcggcgccac cgtcatggcg cagatgacgg tcgccgtggc gctcgaggag ggcggcctcg
    17341 accatcccga ccaccactac gccgatacgc tcgccgcggg ccgcgggctg tgcgacccgg
    17401 cgatggcgcg cctgctgtgc gaggaaggac ccgcgcgcat ccgcgagatg gatgcctggg
    17461 gcgtcggttg ggcgcgggaa ggcggcgccc tgaagcgcgc tttcgcgccg gggcacgacc
    17521 gcgcgcgctg cgtgtacgtc gatttcctca acaccggccc tgcggtgtcg aaaacgctgc
    17581 gcgcgcagct caatcgcgtt cccgagatcc gccgcgtcgg cgacctgctg gtgaccgcgc
    17641 tcgcgctgcg cgggggcgaa tgcgtgggcg cgaccgcgct gcatctggcc agcggcgagc
    17701 cggtgagcat cgccgcgggc gcgacggtgc tcgccaccgg cggcctcacg cgcttgtacc
    17761 ggcgcaacag cgcctcggcc aacatgatgg gcgacggcta cgcgctggca ctcgccgcgg
    17821 gcgccgagct gatcgacatg gagttcgtgc agttctttcc catcgggcac ctcgcgccgc
    17881 gcctcgtggg catggacccg atcatgtggg acccgttccg ctacaagctc ggcgggcggc
    17941 tgctgaacgg ccggcgcgag gagttcatcc accgctacgg cgcaaaggac gacgggcgct
    18001 acgtggtgac gcgcgacgtc gccacctacg ccatcctcaa ggaagccgcc gccgggcgcg
    18061 gctcgccgca cggcggggcg tggctctcgt tcgagcactg caccgaggcc gagctgcgcg
    18121 cggccggtgg gccggtgatc gaccggctcg ccgcgaacgg catcgatttg acgaaacagg
    18181 cgatcgaggt ggcgccaatc gctcactacc acatgggcgg cgtgcgcgtc gatgcaaagc
    18241 tggcgacgcg cgtgccgggc ctgttcgccg cgggcgaggc g
//
LOCUS       DBNHKE010000006         4750 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_821, whole genome shotgun sequence.
ACCESSION   DBNHKE010000006 DBNHKE010000000
VERSION     DBNHKE010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_821"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16O9_00255"
     CDS             <1..299
                     /locus_tag="AC16O9_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515084.1"
                     /translation="AFPPERGELTRGVPTGYAAAPLNTQIAGSSEPPPVWPYAEGKTR
                     GYAFAPLYRTVPEAALRDPALYELLALVDAIRDGRARERNLAEKALEKRLRPAA"
     gene            360..500
                     /locus_tag="AC16O9_00260"
     CDS             360..500
                     /locus_tag="AC16O9_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515085.1"
                     /translation="MGATVAGLLADDGFLNALPGLVLEGSPAVRTPIVLQRLRQLAQL
                     RD"
     gene            783..1142
                     /locus_tag="AC16O9_00265"
     CDS             783..1142
                     /locus_tag="AC16O9_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515086.1"
                     /translation="MKLPANTAPFLYGMAAGAIALAVIGFNFSGWMTPVAAAQHASAR
                     VELAKVEMLTTICVAQFQKDPNAKASLAELMARERWDQADYVSKGGWATMPGGGVAAP
                     SRDVTEACAKTLAKRDV"
     gene            1154..1351
                     /locus_tag="AC16O9_00270"
     CDS             1154..1351
                     /locus_tag="AC16O9_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515087.1"
                     /translation="MKPPVIAVAGIPADWNAIGKSMRAANAPAEPANDPDIAAVQEWE
                     DEGGSVRHLETPPTKPPAKRD"
     gene            complement(1392..1667)
                     /locus_tag="AC16O9_00275"
     CDS             complement(1392..1667)
                     /locus_tag="AC16O9_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515088.1"
                     /translation="MTLRDEIVALRDRIAKAESDRDAWRASGNQEKYIEGYGVVEALE
                     LRLEQLRREGLRASGKDSARPPVCALLGGEPQPAGGGLPGPPEKVEP"
     gene            complement(1805..2029)
                     /locus_tag="AC16O9_00280"
     CDS             complement(1805..2029)
                     /locus_tag="AC16O9_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515089.1"
                     /translation="MTLKDDPAAQQSSARQQKYLVALCTAETLEMQLEQTRLAGLWAS
                     IKFSARALVASPGEPPAADAMSERPVRMPG"
     gene            complement(2026..2730)
                     /locus_tag="AC16O9_00285"
     CDS             complement(2026..2730)
                     /locus_tag="AC16O9_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6515090.1"
                     /translation="MPANRLLAALPRAAYRQLAPSLAPAALVFGDVLYEPGAAMRDVY
                     FPSGSVVSLLTVVDGRSALEVGLVGHEGMVGIPLALGIGVSPVRALVQGGGGALRLSA
                     ARLRQCLRASVPLQRALHRYAAALMAQITQTAACNRFHRVEARLARWLLMTRDRVGAG
                     EFHLTQEFLAHMLGVRRVGVTAAASALQRRGLIGYQRGDIRIVDHAGLEAAACGCYAH
                     VRQRTDAGRRWSQAAP"
     gene            complement(2792..3523)
                     /locus_tag="AC16O9_00290"
     CDS             complement(2792..3523)
                     /locus_tag="AC16O9_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6515091.1"
                     /translation="MVKPRSASRLPPRANRLLASLPDADYQALLPHLEPMAMPLGLAV
                     YESGGLQGFVVFPTDSVVSLLYVLEDGASTEIAVTGREGLVGISLFMGGETTPSRAVV
                     QSAGHGYRVRAAVLKKHFEARGELQHLLLRFTQALITQMTQTAVCNRHHAVEQQLCRW
                     LLLMLDRLSAPELRMTQELIANMLGVRREGVTEAAGKLQALGLIEYRRGRISVLDRPG
                     LEARVCECYAVVKREYDRLLGAAAH"
     gene            3670..4026
                     /locus_tag="AC16O9_00295"
     CDS             3670..4026
                     /locus_tag="AC16O9_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515092.1"
                     /translation="MKFPANTEPFLWGTGAGAIALAIIGFNWGGWMTESTAQQRASAH
                     ADKAVVASLTPICVAQFRKDPKSQASLAVLKGTDRWDQVEFINKGGWAMMPGSTAEPS
                     RDVADACAQALVKPTV"
     gene            4132..>4750
                     /locus_tag="AC16O9_00300"
     CDS             4132..>4750
                     /locus_tag="AC16O9_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR6515093.1"
                     /translation="MDPAALLFGAYRRDALALLLLHPEASLHVREIARAIGKAPGTLL
                     RELNALAQAGVLLRKPLGNQVHFQADPDCPIYEELRGILKKTVGVADVLREALAPLAP
                     RIRAAFVYGSVARGDERARSDLDVMVVGEASFGDVVDALASAQEALRREVNPNVYPAL
                     EFRKKLAAGDPFLKRVLAGRKIFIVGNEDDLGKPAAHRPAQGARRR"
BASE COUNT          713 a         1740 c         1667 g          630 t
ORIGIN      
        1 acgccttccc gccggagcgc ggcgagctca cccgcggggt gccgaccggc tacgccgcag
       61 cgccgctcaa cacccagatc gccggctcga gcgagccgcc accggtctgg ccctacgccg
      121 aaggcaagac ccggggttac gcgttcgcac ccctttaccg caccgtaccc gaagcggcgc
      181 tgcgcgaccc ggcgctctac gagctgctgg cgctggtcga cgcgattcgc gacgggcgcg
      241 cgcgcgagcg aaacctcgcc gagaaggcgc tcgaaaagcg cctgcggccg gcagcgtgat
      301 atgggcgacc cgaacatcga gattctcggc gcgcggccga cccggaactc cgcgcattcg
      361 tgggcgcgac ggtcgcaggc ctgctcgcgg acgacgggtt tctcaatgcg ctgcccggcc
      421 ttgttctcga aggcagccct gcggtacgca cgcccatcgt gctgcagcgc ctgcggcagc
      481 tcgcgcaatt gcgcgactga ggacgccgcg cctgctcgcg caggagacca gctacttctt
      541 ccccgcctgc ttcgccgccc gccgcagctt cttcttgatc ttttgcttgc gcgcccggtt
      601 gcgacggtcg cgtgccgtgt gtctcatggt cggttcctcc tggaaacgcg cagtatatgc
      661 gcccgcccgc gcccggccgc ccgctgtgta cgccagcgca cagagcgcgc cgcggaacgg
      721 gcgcaccctg aaggctcgag gacccggcgc accgggtccc ggtcaacgaa tcgaggtatc
      781 tcatgaaact tcccgccaac accgcaccat ttctttacgg catggccgcg ggcgcgatcg
      841 cgctcgccgt catcggcttc aacttcagcg gctggatgac ccccgtcgcg gccgcccagc
      901 acgccagcgc gcgcgtggag ctggccaagg tcgagatgct caccacgatc tgcgtggccc
      961 agttccagaa ggaccccaac gcgaaggcga gcctcgccga gctgatggcc cgggagcgct
     1021 gggaccaggc cgactacgtg agcaagggcg gctgggcgac gatgcccggc ggcggcgtcg
     1081 ccgcgccgag ccgcgacgtc accgaggcct gcgccaagac gctcgccaag cgcgacgtgt
     1141 aggacacggc gccatgaaac cacccgtcat cgccgttgca ggaatccccg ccgattggaa
     1201 cgccatcggc aagtcgatgc gcgccgccaa cgcgccggcc gagcccgcca acgatccgga
     1261 catcgcagcg gtgcaggaat gggaggacga aggcggcagc gtcaggcacc tggaaacgcc
     1321 accgacgaag ccgcccgcca agcgggactg agtcctccgc cgcgcgcggc ggcgacgcta
     1381 gcccgtatgc ctcaaggctc gaccttctcc ggcggacccg gcaagccgcc gcccgcgggc
     1441 tgcggctcgc cgcccagcaa agcgcagacg gggggacgag cggaatcctt cccggaagcc
     1501 cgcaggcctt cccgccgcag ctgctcgagc cgcagctcca gggcctcgac cacgccatag
     1561 ccctcgatgt acttctcctg gttccccgag gcgcgccagg cgtcgcggtc cgactcggcc
     1621 ttggcgatgc ggtcccgcag ggcgacgatt tcatctctga gtgtcatgac gcagcctggg
     1681 accagcggcg gccggcgtcc gtgcgctggc gcacatgcgc gtagcagccg cgggcggcgg
     1741 cttccagccc ggcgtggtcg gcgatccggt tgtcgccgcg gtggtccagg cactccgggc
     1801 ggtgctagcc gggcatgcgg accggccgtt ccgacatggc gtccgccgcc ggcggctcgc
     1861 ccggcgaggc caccagggcg cgggccgaaa acttgatcga ggcccacagg cccgccaggc
     1921 gcgtctgctc gagctgcatc tccagggttt cggcggtgca gagggctacc aggtacttct
     1981 gctgcctggc ggaggactgc tgtgcggcgg gatcgtcttt gagggtcatg gcgcagcctg
     2041 ggaccagcgg cggccggcgt ccgtgcgctg gcgcacatgc gcgtagcagc cgcaggcggc
     2101 ggcttccagc ccggcatgat cgacgatgcg gatgtcgccg cgctggtagc cgatgaggcc
     2161 gcgccgctgc agggcggagg ccgccgcggt gaccccgacc cggcgcacgc cgagcatgtg
     2221 cgcgaggaac tcctgcgtga ggtggaattc ccccgcgccg acgcgatcgc gcgtcatcag
     2281 cagccagcgc gcgaggcgcg cctcgacccg gtgaaagcga ttgcacgcgg cggtctgggt
     2341 gatctgcgcc atcagcgcgg cggcgtagcg atgcagcgcg cgctgcagcg ggacgctcgc
     2401 ccgcaggcac tggcgcaggc gcgcggcgct caggcgcagc gcgccgccgc cgccctgcac
     2461 cagggcgcgc accggcgaga cgccgatgcc gagcgcgagc gggatcccga ccatgccctc
     2521 gtgccccacc agcccgactt cgagcgcgct gcggccgtcg acgacggtga gcagcgacac
     2581 taccgagccg ctggggaagt agacgtcgcg catcgcggcg ccgggctcgt agagcacgtc
     2641 gccgaagacg agcgccgcgg gcgcgaggct cggcgcgagc tgccggtagg ccgcgcgcgg
     2701 cagcgcggcg agcaggcggt tggcgggcat gcgccattgt gggcagcgcc gcggcgcgcg
     2761 tatgttcgcg cgcgcacata gcgcggctct ttcagtgcgc ggccgcgccg agcagccggt
     2821 cgtactcgcg cttgaccacc gcgtagcact cgcacacgcg cgcctcgagc cccgggcggt
     2881 cgagcaccga gatgcgcccg cgccggtact cgatcagccc cagcgcctgg agcttgccgg
     2941 ccgcctcggt gacgccctcg cggcgcacgc cgagcatgtt ggcgatcagc tcctgggtca
     3001 tgcgcagctc gggcgcggac agccggtcga gcatcagcag cagccagcgg cacagctgct
     3061 gctccaccgc gtggtggcgg ttgcacaccg cggtctgcgt catctgcgtg atcagcgcct
     3121 gggtgaagcg aagcagcagg tgctgcagct cgccgcgcgc ttcgaagtgc tttttcagca
     3181 ccgccgcgcg cacgcggtag ccgtggccgg cgctctgcac cacggcgcgg ctcggcgtgg
     3241 tctcgccgcc catgaacagc gagatcccga ccaggccttc gcgcccggtg accgcgatct
     3301 cggtcgaggc gccgtcctcg agcacgtaga gcagcgacac gacgctgtcg gtcgggaaca
     3361 ccacgaagcc ctgcaggccg ccggattcgt acaccgccag cccgagcggc atggccatcg
     3421 gctccaggtg cggcagcagc gcctggtaat cggcatcggg gagcgacgcg aggagccggt
     3481 tggccctcgg cggcaggcgg ctggcgctgc gcggcttgac caccgggcca tgctacgcgc
     3541 atcgcggcgc cggcgcacgc gctctatgta cggcagcgca cagaaggccc cgcgcaatgc
     3601 gcgcaccctg ccatgaccga agcgcgcggc gcctgcgccg cgccttcaac cacgaatcga
     3661 ggagatgcca tgaaatttcc tgccaatacc gagccgttcc tctggggcac cggcgcgggc
     3721 gccatcgcgc tcgccatcat cggcttcaac tggggcggct ggatgaccga aagcacggcg
     3781 cagcagcgcg ccagcgcgca cgcggacaag gcggtggtcg cttcgctcac cccgatctgc
     3841 gtggcccagt tccgcaagga cccgaaatcg caggcgagcc tcgccgtgct gaagggcacc
     3901 gatcgctggg accaggtcga gttcatcaac aagggcggct gggcgatgat gcccggcagc
     3961 accgccgagc cgagccgcga cgtcgccgac gcctgcgcgc aggcgctcgt caagcccacc
     4021 gtctaggacg cggcttcagg acgcgccgag tacaccttcg cacccacgcc ggcccaaggt
     4081 tgacggtcgt tgcgctaaag gctacagtcg tagctcttta agcaacgacc gatggacccc
     4141 gccgcgctcc ttttcggcgc ctaccgccgc gacgcgctcg cgctgctcct cctgcacccg
     4201 gaggcgtcgc tgcacgtgcg cgagatcgca cgcgccatcg gcaaggcgcc gggcacgctg
     4261 ctgcgggagc tgaacgcgct cgcgcaggcg ggcgtgctgc tgcgcaagcc cctcggcaac
     4321 caggtccact tccaggccga ccccgactgc ccgatctacg aggagctgcg cggcattctc
     4381 aagaagaccg tgggcgtcgc ggacgtgctg cgcgaggcgc tcgcgccgct cgcgccgcgc
     4441 atccgcgccg cgttcgtcta cggctcggtg gcccggggcg acgagcgcgc gcgcagcgac
     4501 ctcgacgtca tggtggtggg cgaggcgagc ttcggcgacg tggtcgacgc gctcgcgagt
     4561 gcccaggagg cgctgcgccg cgaggtcaac ccgaacgtct atcccgcgtt ggagttcagg
     4621 aagaagctcg ccgccgggga cccgttcctc aagcgggtcc tggccgggcg caagatcttc
     4681 atcgtgggga acgaggatga ccttggaaaa cctgctgcgc accggccagc tcaaggcgca
     4741 cgccgccgat
//
LOCUS       DBNHKE010000007         9674 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_159659, whole genome shotgun sequence.
ACCESSION   DBNHKE010000007 DBNHKE010000000
VERSION     DBNHKE010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_159659"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="tldD"
                     /locus_tag="AC16O9_00305"
     CDS             <1..1018
                     /gene="tldD"
                     /locus_tag="AC16O9_00305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR6515094.1"
                     /translation="EKLERLCRARDPRVGQVMAQLGGEYEVVLIARADGLRVADVRPL
                     VRLSVQVIAEQHGRRESGSAGGGGRTDYAMFTDAALEAYARQAVDQALINLEARPAPA
                     GTMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSAFSGRMGERVAAPGVTVVDDGTIP
                     DRRGSLTVDDEGNPTRRTVLIEDGVLRGLLQDSLNARLMKMPLTGNGRRESYAHLTLP
                     RMTNTFMVGGAHDPGEIIRSVKKGLYAVNFGGGQVDITSGKFVFSAAESYLIEDGKVT
                     APVKGATLIGNGPDVLTRVSMVGNDFRLDPGIGTCGKEGQSVPVGVGQPTLKIDGLTV
                     GGTA"
     gene            1015..1872
                     /locus_tag="AC16O9_00310"
     CDS             1015..1872
                     /locus_tag="AC16O9_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR6515095.1"
                     /translation="MSLASNATQHFEDADALAARIADGSSIGLPPDYSPVAMAVVRAL
                     VRRNAKDLKLIGVPVLGLCADILIGAGCVREVETSAVTLGEAGLAPRFSEAAGQKSLK
                     IIDATCPMIHTGLQATEKGVPFLPLRGVLGSDLVKRHPDWKTIDNPYAKGDPILLAKA
                     ICPDVALFHARWADEAGNVWVGRRGELATIAHASRACYVTYEELKPGDMLEDEILAPG
                     CIGSVYISAVAPAPRGAWPLGVPGLYGIDDAHVQAYARLAKTADGFRKYLDEFVWNPT
                     SEKNSLPVS"
     gene            1899..2582
                     /locus_tag="AC16O9_00315"
     CDS             1899..2582
                     /locus_tag="AC16O9_00315"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR6515096.1"
                     /translation="MAVGAASPIPATGAFLLKRQNPALRISLLQKRTGNPFTQGSREL
                     FDLAGQGRIDVFFLGGAQIDGEANINLVEAEGKRFPGSFGSAYMYFTAGKTILFREEH
                     SPRVLVKNVEFVSAPGWSPPGVERRGGPAALLTGKALFRWQAGKRRFLLESVHPGGTA
                     EDVRASTGFVFDASTRVPQTAEPTLAQLRELRGPVAQVIAENYPEFARRVWGGEGGRS
                     FAAARSATQ"
     gene            2610..3245
                     /locus_tag="AC16O9_00320"
     CDS             2610..3245
                     /locus_tag="AC16O9_00320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6515097.1"
                     /translation="MAVLRIARMGNPVLSQVAAPVADPGAPDIRRLAADMIETLEDIG
                     ASGLAAPQVFDSRRVVVYRMIAARIPKGSGIEPRPWTVMVNPVITPKSSAKTPVWERC
                     LSIPGLHGKVPRHSRIAIRYQTPEGQTVSHDAHSSWAALLQHECDHLDGIVYPMRMAD
                     LGLLAYNDEPGQLAREVAADPSGIDPLFVDLVARWPARERWLGAASAPAPT"
     gene            complement(3223..4713)
                     /gene="glpK"
                     /locus_tag="AC16O9_00325"
     CDS             complement(3223..4713)
                     /gene="glpK"
                     /locus_tag="AC16O9_00325"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR6515098.1"
                     /translation="MKHILALDQGTTSSRAIVFDERGAPVSVAQEEFAQIYPQPGWVE
                     HDAKAILATQRAVAREALRRAAVPPKSVAAIGITNQRETTLVWDRQSGEPIANAIVWQ
                     DRRTAPACRDLKEAGAESLVRERTGLVIDPYFSATKIGWLLDNVPGARERAERGELAF
                     GTVDTWLIWHLTGNRTHVTDVTNAARTMLFNVHTQDWDEELLRLLRVPRALLPDVHPS
                     THAFGIVPKDVFGEPLIIGGVAGDQQAAMFGQACHAPGMAKNTYGTGCFMLLHTGSEM
                     VQSSHGLVSTPCAHLKAKEFALEGSVFVAGAVVQWLRDGLGFFNSSSEIEALANSVLD
                     SGDVCIVPAFTGLGAPHWDPEARGSIFGLTRGTTRAHIARAALESIAFQSADILEAMQ
                     NDSKMELKELRVDGGATANDLLMQFQADVLGVPVVRPKVLETTALGAAYLAGLTVNLW
                     KSRDEIAAQWQVEKRFEPRMEAARRGALMARWREAVGRSLKWAQER"
     gene            complement(4718..6298)
                     /locus_tag="AC16O9_00330"
     CDS             complement(4718..6298)
                     /locus_tag="AC16O9_00330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR6515099.1"
                     /translation="MQPAREELVERLARSPALDLAVIGGGATGLGVALDAAARGLSVA
                     LFEAEDFAKGTSSRATKLVHGGVRYLAQGNVALVREALRERARLLQNAPHLVQPLAFV
                     LPLYGARGRWLDSWFYGAGLTLYDLLAGGARLGRTQFLGHAAALERAPGLRADDLAGG
                     LQYWDGQFDDARLAVALARTAIARGAIVVNHCAAVGMTHDSAGKINGVLVEDRARGQQ
                     HRIGARCVVNAAGVWVDSVRRMDRAGRGALLAPSQGVHLVVDREFLPSRSAVLVPRTR
                     DGRVMFAVPWLGKTILGTSDTPRTDVPGEPEPLPGEVELILGEAARVLERAPTRADVR
                     SVWVGMRPLVKPPDEDPGDTKALSREHAVLVERTGLVTVTGGKWTTYRAMAEDVLQHA
                     AEAGLLPARPAGATSTLPLVGAPARGEHALSEAPGLHLYGTEAQAVQALPGATRELAP
                     GLTEAMVRFAARHEYARSVEDALARRSRLLFLDARLAAEVAERVAAVLAEELGDAFDA
                     ARSLRDFRALAARYASVE"
     gene            6349..6903
                     /locus_tag="AC16O9_00335"
     CDS             6349..6903
                     /locus_tag="AC16O9_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HIR6515100.1"
                     /translation="MPISEVERELTHTRRVRYEGYKRADGLWDIEAHITDVKNHDFTL
                     KTGVRRAGLPVHDMTVRVTIDRKFNIVDAIAVADAVPYPGGCEAAAPEYAKLVGLNLL
                     KKFRHGLKERLGGVQGCTHITEMLAALPTAAIQTFAGEGREDRPAGRKPFQLDHCLAM
                     DTTGESVKKWYPEWYMVKEEKAGA"
     gene            6900..8063
                     /gene="sucC"
                     /locus_tag="AC16O9_00340"
                     /pseudo
     CDS             6900..8063
                     /gene="sucC"
                     /locus_tag="AC16O9_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
     gene            8171..9055
                     /gene="sucD"
                     /locus_tag="AC16O9_00345"
     CDS             8171..9055
                     /gene="sucD"
                     /locus_tag="AC16O9_00345"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR6515101.1"
                     /translation="MSILVNKHTRVMTQGITGKTGMFHTQTSRAYANGRECYVAGVNP
                     KKAGEDFEGIPIFGTVRDAKEKTGATVSVIYVPPPFAAAAIWEAVEADLDLAICITEG
                     IPVRDMIEVRDRMRKQNRRTLLLGPNCPGVITPGELKIGIMPGHIHKKGRVGVVSRSG
                     TLTYEAVAQLTELGIGQSTAVGIGGDPVNGLKHVDVLKLFNDDPGTDAVIMIGEIGGS
                     DEEAAAEWVKQNMKKPVVGFIAGVTAPPGKRMGHAGAIISGGKGTAQEKLAVMQACGM
                     KVTRNPAEMGRLLKSVLK"
     gene            9057..>9674
                     /locus_tag="AC16O9_00350"
     CDS             9057..>9674
                     /locus_tag="AC16O9_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6515102.1"
                     /translation="MLRRLLANALIASGARAERGARLELACRRYRAAAALAPRYAPAH
                     LNLGAALEALGDAAGAFAAYQAALAAGGDNPFARYNAGRLHYARGELETAETHLRHAL
                     QRKPDFVEALITLAAVLDARRDPNDAAALLQQALALAPGDGGAWFNYGELLWRLARYD
                     EAEDALRRALEIEPRFIPAWHLLGNLLRGTSRIAEALQTFAAAGRA"
BASE COUNT         1567 a         3300 c         3478 g         1329 t
ORIGIN      
        1 ggagaagctg gagcggctgt gccgggcgcg cgatccgcgc gtcggccagg tgatggcgca
       61 gctcggcggc gagtacgagg tggtgctgat cgcgcgcgcc gacggcctgc gcgtggccga
      121 cgtgcgcccg ctggtgcgcc tgtcggtgca ggtgatcgcc gagcagcacg ggcgccgcga
      181 aagcggcagc gcgggcggcg gcgggcgcac cgattacgcg atgttcaccg acgcggcgct
      241 cgaagcatac gcgcgccagg cggtggacca ggcgctcatc aatctggagg cgcgcccggc
      301 gcccgccggc accatgaccg tggtgctcgg cccgggctgg ccggggatcc tgctgcacga
      361 ggcgatcggc cacggcctgg agggcgactt caaccgcaag ggcagctcgg cgttctcggg
      421 gcgcatgggc gagcgcgtcg cggcgcccgg cgtcacggtg gtggacgacg gcacgattcc
      481 cgaccggcgc ggctcgctca ccgtggacga cgagggcaac ccgacgcggc gcacggtgct
      541 gatcgaggac ggcgtgctgc gcgggctgct gcaggacagc ctgaacgcgc gcctgatgaa
      601 gatgccgctc accggcaacg gccggcgcga gtcctacgcg cacctcacgc tgccgcggat
      661 gacgaacacc ttcatggtcg ggggcgcgca cgacccgggc gagatcatcc gcagcgtgaa
      721 gaagggcctg tacgcggtga acttcggcgg cggccaggtc gacatcacca gcggcaagtt
      781 cgtgttctcg gcggccgagt cctatctgat cgaggacggg aaggtgaccg cgccggtgaa
      841 gggcgcgacg ctgatcggca acggcccgga cgtgctcacg cgcgtctcga tggtcggcaa
      901 tgattttcgt ctcgaccccg gcatcggcac ctgtggcaag gaggggcaga gcgtgccggt
      961 gggggtgggg cagccgacgt tgaagatcga cgggctgacg gtggggggga cggcgtgagc
     1021 ctggcgtcga acgcgacgca gcacttcgag gatgccgacg ccctcgcggc gcggatcgcc
     1081 gacggctcgt cgatcgggct gccgccggac tactcgccgg tggcgatggc ggtggtgcgc
     1141 gcgctggtgc gaaggaacgc aaaggatctg aagctcatcg gcgtcccggt gctcgggctt
     1201 tgcgccgaca tcctgattgg cgcgggctgt gtgcgggagg tcgagaccag cgcggtgacg
     1261 ctgggggagg cggggctcgc gccacgcttc agcgaggccg ccgggcagaa gtcattgaag
     1321 atcatcgatg ccacctgccc gatgatccac acggggctgc aggcgaccga gaagggcgtg
     1381 ccgttcctgc ctttacgcgg cgtgctcggc tcggacctcg tcaagcgcca tccggactgg
     1441 aagacgatcg acaatcccta tgccaagggc gatccgatcc tgctggcgaa ggcgatctgt
     1501 ccggatgtcg cgctgttcca cgcgcgctgg gcggacgagg cgggcaacgt ctgggtcggg
     1561 cggcgcggcg agctcgccac catcgcgcat gcgtcccggg cctgctacgt cacttacgaa
     1621 gagctgaagc ccggcgacat gctggaggac gagatcctcg cgccggggtg catcggctcg
     1681 gtctacatca gcgcggtggc gccggcgccg cgcggcgcct ggccgcttgg cgtgccgggg
     1741 ctgtacggca tcgacgacgc gcacgtgcag gcttatgcac gattggcgaa gacggccgac
     1801 gggttcagga aatatctcga cgagttcgtg tggaacccta ccagcgagaa gaactccttg
     1861 cctgtgtcat agcgcgcctg atcggcgacg cgcggcatgt ggcggtgggg gcggcgtcgc
     1921 cgattccggc cacaggggcg tttctgctga agcggcagaa ccctgcgctg cgcatctcgc
     1981 tgctgcaaaa gcgcaccggc aatccgttca cccaaggctc gcgcgagctg ttcgacctgg
     2041 cggggcaggg gcgcatcgac gtcttcttcc tgggcggggc gcagatcgac ggcgaggcga
     2101 acatcaacct cgtcgaagcc gagggcaagc ggtttccggg ttccttcggc tcggcgtaca
     2161 tgtacttcac cgcgggcaag acgatcctgt ttcgcgagga gcattcgccg cgcgtgctgg
     2221 tgaagaacgt ggagttcgtt tccgcgccgg gctggagccc gccgggcgtc gagcggcgcg
     2281 gcgggccggc ggcgctgctc acgggaaagg cgctgtttcg ctggcaggcc gggaagcgcc
     2341 gattcctgct cgaaagcgtg catcccggcg gcaccgccga ggacgtgcgc gcgagcacgg
     2401 gtttcgtctt cgatgcgtcg acgcgggtgc cgcagaccgc ggaaccgacg ctggcgcagc
     2461 tgcgcgagct gcgcggtccg gtagcgcagg tcatcgcgga gaactatccg gaattcgcgc
     2521 ggcgggtatg gggcggagag ggcgggcggt cgttcgccgc cgctcgatcc gccacgcaat
     2581 agcggcaacc gaacgagggg gaggcaagca tggccgtgtt acggatcgcg aggatgggca
     2641 acccggtgct gtcgcaggtt gcggcgccgg tcgcggaccc gggcgcgccg gacatccggc
     2701 gcctcgccgc cgacatgatc gagacgctcg aggacatcgg cgcgagcggc ctcgccgcgc
     2761 cgcaagtgtt cgacagcagg cgcgtcgtgg tctaccgcat gatcgccgcg cgcattccca
     2821 agggctcggg catcgagccg cgcccctgga cggtgatggt caacccggtc atcaccccca
     2881 agtcttcggc gaagacgccg gtgtgggagc gctgcctgtc gatccccgga ctgcacggca
     2941 aggtgccgcg ccattcgcgc atcgcgatca gataccagac gcccgagggg caaacggtat
     3001 cgcatgacgc gcacagttcg tgggcggcgc tgctgcagca cgagtgcgac cacctcgacg
     3061 gcatcgtcta tccgatgcgc atggcggatc tggggctgct cgcctacaac gacgaacccg
     3121 ggcagctcgc gcgcgaggtc gcggccgacc cgagcggcat cgatcccctg ttcgtcgatc
     3181 tggtcgcgcg ctggccggca cgcgagcgct ggctcggcgc cgctagcgct cctgcgccca
     3241 cttgagcgag cggcccaccg cctcgcgcca gcgcgccatc agcgcgccgc gccgcgccgc
     3301 ctccatgcgc ggctcgaagc gcttctccac ctgccactgc gcggcgatct cgtcgcgcga
     3361 cttccataga ttgacggtca agccggcgag ataggcagcc cccaaagccg tcgtctccag
     3421 cactttcggc cgcaccaccg gcacgcccag cacgtcggcc tggaactgca tcagcaggtc
     3481 gtttgcggtc gcgccgccgt ccacgcgcaa ttccttcagc tccattttcg aatcgttctg
     3541 cattgcttcg agaatgtccg cgctctggaa cgcgatcgac tccagcgccg cgcgcgcgat
     3601 gtgcgcgcgc gtcgtcccgc gcgtaagccc gaatatcgac ccgcgcgctt cgggatccca
     3661 gtgcggcgcg ccaagcccgg tgaacgccgg cacgatacag acgtcgccgg aatccagcac
     3721 gctgttcgcc aaagcctcga tctcggagga cgaattgaag aaccccagcc cgtcgcgcag
     3781 ccactgcacc accgcgcccg cgacgaacac gctgccttcc agcgcgaact ccttcgcctt
     3841 caagtgcgcg cagggcgtcg acaccaagcc gtgagacgac tgcaccatct cgctgccggt
     3901 gtgcagcagc atgaagcaac ccgtcccgta cgtgttcttc gccatccccg gcgcatggca
     3961 ggcctgcccg aacatcgccg cctgctggtc gccggcgacg ccgccgatga tcaaaggctc
     4021 gccgaagacg tccttcggca cgatgccgaa ggcgtgcgtg gaaggatgca cgtcgggcag
     4081 caaggcgcgc ggcacgcgca acagccgcag cagctcctcg tcccagtcct gcgtgtgcac
     4141 gttgaacagc atggtgcgcg cggcgttggt cacgtcggtg acgtgcgtgc ggttgccggt
     4201 caggtgccag atgagccagg tgtccacggt gccgaaggcg agctcgccgc gctcggcgcg
     4261 ttcgcgcgcg ccgggcacgt tgtcgagcag ccagccgatc ttggtggcgc tgaagtacgg
     4321 atcgatgacc aggcccgtcc gctcgcgcac caggctctcg gcgcccgcct ccttcagatc
     4381 gcggcaggcc ggcgccgtgc gccggtcctg ccagacgatg gcgttggcga tcggctcgcc
     4441 gctctggcgg tcccagacga gcgtggtctc gcgctggttg gtgatgccga tcgccgccac
     4501 gctcttcggc ggcaccgccg cgcggcgcag ggcctcgcgc gccaccgcgc gctgcgtggc
     4561 gaggatcgcc ttcgcatcgt gctcgaccca gccgggctgc gggtagatct gcgcgaattc
     4621 ctcctgcgcg acgctcaccg gcgcgccccg ctcgtcgaag acgatggcgc gcgagctggt
     4681 ggtgccctgg tccaacgcga ggatgtgctt catgcgttca ttctactgag gcgtaacgcg
     4741 cggccaaggc gcggaaatcg cgcagcgagc gcgccgcgtc gaacgcatcg ccgagctcct
     4801 cggcgagcac cgccgcgacg cgctcggcga cctccgccgc gaggcgcgcg tcgagaaaca
     4861 gcaggcgcga gcggcgcgcg agggcgtcct cgacgctgcg cgcgtactcg tgtcgcgccg
     4921 caaagcgcac catcgcctcg gtgagcccgg gcgcgagctc gcgtgtcgcg ccgggcaggg
     4981 cctgcaccgc ctgcgcttcg gtgccgtaga ggtgcaggcc cggcgcttcg ctcaaggcgt
     5041 gttcgccgcg cgcaggcgcg ccgacgagcg gcagcgtcga agtggcgccg gccgggcgcg
     5101 caggcagcag cccggcttcg gcggcgtgct gcagcacgtc ctcggccatg gcgcggtagg
     5161 tggtccactt gccgccggtg acggtcacca gcccggtgcg ctcgacgagc accgcgtgct
     5221 cgcgcgagag cgccttggtg tcgccggggt cctcgtcggg cggcttcacc agcggccgca
     5281 tcccgaccca gaccgagcgc acatccgccc gcgtcggcgc acgctcgagc acgcgcgcgg
     5341 cttcgcccag gatgagctcg acttctcccg gcaaaggttc gggctccccg gggacgtcgg
     5401 tgcgcggggt atcggaagtg ccgagaatgg tcttgccgag ccagggaacc gcaaacatca
     5461 cgcgcccgtc gcgggtcctg ggcaccagca ccgcgctgcg gctgggcagg aactcgcggt
     5521 cgacgacgag gtgcacgccc tggctcggcg cgaggagtgc gccgcgtccg gcgcgatcca
     5581 tgcgccgcac gctgtcgacc cagacgccgg cggcgttgac cacgcagcgc gcgccaatgc
     5641 ggtgctgctg gccgcgggcg cggtcctcca cgagcacgcc gttgatcttg cccgcgctgt
     5701 cgtgggtcat gcccacggcg gcgcagtgat tgacgacgat cgcgccgcgc gcgatcgcgg
     5761 tgcgcgcgag cgccacagcg aggcgcgcgt cgtcgaactg gccgtcccag tactgcagcc
     5821 cgcccgccag atcgtcggca cgcaggccgg gcgcgcgctc caatgcggcg gcgtggccga
     5881 gaaattgggt gcgcccgagg cgcgcgccgc cggctaggag gtcgtacagc gtgagcccgg
     5941 caccgtagaa ccagctgtcg agccagcgcc cgcgcgcccc gtacagcggc aggacgaaag
     6001 cgagcggctg cacgaggtgg ggtgcgtttt gcagcaggcg ggcgcgctcg cgcagtgcct
     6061 cgcgcaccag cgccacgttg ccctgggcga ggtagcgcac gcccccatgc accagcttgg
     6121 ttgcgcggga cgacgtgccc ttggcgaaat cctcggcctc gaagagcgcc accgagagcc
     6181 cgcgcgcggc ggcgtcgagc gccacgccga ggcccgtggc gccgccgccg atcaccgcca
     6241 ggtcgagcgc cggggagcgc gcgagccgct cgaccagctc ttcgcgcgca ggctgcacgg
     6301 cggcgattct agcaaggcgc cgatgctaaa cttcgcggcg ccatgcccct gcccatctcc
     6361 gaagtcgagc gcgagctcac ccacacgcgg cgcgtgcgct acgaaggcta caagcgcgcc
     6421 gacggcctct gggacatcga ggcccacatc accgacgtca agaaccacga tttcacgctg
     6481 aagaccggcg tgcgccgcgc gggcctgccg gtgcacgaca tgaccgtgcg cgtcaccatc
     6541 gaccgcaaat tcaacatcgt cgacgcgatc gcggtggccg atgccgtgcc ctatcccggc
     6601 ggctgcgagg ccgcggcgcc cgagtacgcg aagctcgtcg gcctgaacct gctgaagaaa
     6661 ttccgccacg gcctgaagga acggctgggc ggggtgcagg gctgcacgca catcaccgag
     6721 atgctggccg cgctgccgac ggcggcgatc cagacctttg ccggggaagg gcgcgaggac
     6781 cggcccgccg gcagaaaacc tttccagctc gaccactgcc tcgccatgga caccaccggc
     6841 gagtcggtga agaagtggta tcccgagtgg tacatggtga aggaagaaaa ggcgggggca
     6901 tgaagatcca cgagtaccag ggcaaggaaa tcttccgcaa gttcggcgtg cccacgccgc
     6961 gcggcgtcgc cgcgttcagc gtcgacgacg cggtgcgcgc cgccgagacg ctcggcggca
     7021 aggtgtgggt ggtgaaggcg cagatccacg ccggcggccg cggcaagggc ggcggcgtga
     7081 aggtggccaa atcgaccgac gaagtgcgcg cgcacgcgaa gaagatcctc ggcatgcagc
     7141 tcgtcacgca ccagacgggt ccgcagggtc agaaagtgcg gcgcctgctg atcgaggagg
     7201 gcgccgacat cgccaaggag ctctacgtcg gcatggtggc cgaccgtggc acgcagcgcg
     7261 tggtgctgat ggcctcgtcc gagggcggca tggacatcga ggaagtggcg gcgaagacgc
     7321 cgcataagat ccacaaggtg ttcatcgacc cggaagcggg cctggccgag ggcgaggcgc
     7381 gcgaggtggc gcgcagaatc ggcgtgcccg aagcttcgct cggacaggcc gcggagctgc
     7441 tgcgcagtct ttatcgctgc tactgggaca ccgacgcttc gctcgtcgag gtgaacccgc
     7501 tggtcgtcac cggcgacggg cgcgtcgtgg cgctggacgc gaagctcact ttcgacgaca
     7561 acgcgctctt tcgccacgag gacatcgtcg cgctccgcga cctggacgag gaggacccgc
     7621 tcgagatcga ggcgtcgaag cacgacctct cgtacatctc gctggacggc aacatcggct
     7681 gcatggtgaa cggcgccggg ctcgccatgg cgaccatgga cacgatcaag ctctacggcg
     7741 gcgccccggc caacttcctc gacgtcggcg gcggcgcgac ggccgagaag gtcacggccg
     7801 ccttcaagat catgctctcc aatcccaagg tgaaggcgat cctcgtcaac atcttcggcg
     7861 gcatcatgaa gtgcgacacc atcgccgcgg gcgtagtgac ggcggcgcgc gaggtgcacc
     7921 tcgcggtgcc gctcgtcgtg cgcatgaagg gcaccaacga ggagctcggc aagaagatcc
     7981 tcaaggactc cggcctgccg atcatctccg ccgacaacat ggcgcaggcc gggcaaaggg
     8041 cggtcgcggc gatcagaaag aaaaagccgg caaagaaaaa gccggcaaag aagaagccgg
     8101 tgaagaagaa gcccgtgaag aaggcgcccg cgcgcaaggc gaagaaaaag atcgcgaaga
     8161 ggaagaagaa atgagcatcc tcgtgaacaa gcacaccagg gtgatgaccc agggcatcac
     8221 cggcaagacc ggcatgttcc acacccagac cagccgcgcc tacgccaacg ggcgcgagtg
     8281 ctacgtggcc ggggtgaacc cgaagaaggc gggcgaggac ttcgagggca tcccgatctt
     8341 cggcacggtg cgcgacgcca aggaaaagac cggcgccacc gtgtcggtga tctacgtgcc
     8401 gccgccgttc gccgccgcgg cgatctggga agcggtggag gccgatctcg atctggccat
     8461 ctgcatcacc gaaggcatcc cggtgcgcga catgatcgag gtgcgcgacc gcatgagaaa
     8521 gcagaaccgc aggacgctgc tgctcgggcc caactgcccc ggcgtcatta cgccgggcga
     8581 gctgaagatc ggcatcatgc cggggcacat ccacaagaag ggccgcgtgg gcgtggtgtc
     8641 gcgctccggc acgctcacct acgaagccgt ggcacagctc accgagctcg gcatcggcca
     8701 gtcgacggcc gtgggcatcg gcggcgaccc ggtgaacggc ctgaagcacg tcgacgtgct
     8761 gaagctcttc aacgacgatc ccggcaccga cgccgtgatc atgatcggcg agatcggcgg
     8821 ctcggacgag gaggccgcgg ccgagtgggt gaagcagaac atgaaaaagc cggtggtggg
     8881 cttcatcgcc ggcgtcactg cgccgccggg aaagcgcatg ggccacgctg gcgccatcat
     8941 ctccgggggc aagggtacgg cgcaggaaaa gctcgccgtg atgcaggcct gcggcatgaa
     9001 ggtgacgcgc aacccggcgg agatgggccg gctgctgaag tcggtcctga agtagggtgc
     9061 tgcgccggct gctcgcgaac gcgctgatcg cgagcggcgc ccgggcggag cgcggcgccc
     9121 gcctcgagct cgcctgccgg cgctaccggg cggccgcggc gcttgcgccg cgctacgcgc
     9181 cggcgcatct caaccttggc gcggcgctcg aagcgctcgg cgatgccgcc ggcgcctttg
     9241 ccgcgtacca ggcggccctc gcggccggcg gcgacaaccc gtttgcccgt tacaacgccg
     9301 gacgcctgca ctacgcgcgc ggcgagctcg agacggccga aacgcacctg cgccacgcgc
     9361 tgcagcgaaa gcccgacttc gtcgaagccc tgatcacgct ggccgcggta ctcgacgcgc
     9421 gtcgcgaccc gaatgacgcc gcggcgctcc ttcagcaggc gctcgcgctg gcgccgggcg
     9481 acggcggcgc ctggttcaac tacggcgagc tgctatggcg cctcgcgcgc tacgacgagg
     9541 ccgaagacgc attgcggcgc gcgctcgaga tcgagccgcg cttcattccc gcctggcacc
     9601 tgctcggcaa cctgctgcgc ggcacctcgc gcatcgccga ggcgctgcag actttcgccg
     9661 ccgcggggcg ggcc
//
LOCUS       DBNHKE010000008        41215 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1164729, whole genome shotgun sequence.
ACCESSION   DBNHKE010000008 DBNHKE010000000
VERSION     DBNHKE010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 41215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 41215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41215
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1164729"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="AC16O9_00355"
     CDS             <1..723
                     /locus_tag="AC16O9_00355"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR6515103.1"
                     /translation="AERLYRAAAQRAHLEPVGIGCHIGSLLADPAPWIAAAERVIELA
                     ERITQAGIPLQHVDVGGGIGIRYRDEPQRTIEEFLSGALRALGERDEMLIVDPGRSIV
                     GNAGVLLTRVEYVKPGSAKNFVVVDAAMNDLMRPALYDAWHEVRQVREPQPGAATAVY
                     DVVGPICESADFLARDRTLVAHAGELLALMSAGAYGMSMSSNYNSRPRSAEVMVDGAS
                     AHLVRRREEVRALFAGESIPTW"
     gene            749..1540
                     /locus_tag="AC16O9_00360"
                     /pseudo
     CDS             749..1540
                     /locus_tag="AC16O9_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            complement(1562..2344)
                     /locus_tag="AC16O9_00365"
     CDS             complement(1562..2344)
                     /locus_tag="AC16O9_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6515104.1"
                     /translation="MSEAQNHIGETDLHAWADGCLAEGRRVEVEAWLAAHPEDAERAA
                     AYRTLNEQLRAHFEPVLDEPVPERLEHAALRRRRWSVAAAVAGWLALGVTIGGVAGWQ
                     LHARYAPVPLARTETPVLARRAALAHATYAPEVRHPVEVGADQEQHLVAWLSKRLGGE
                     VRAPRLDAEGMTLVGGRLLPRDPGPVAQFMYQSAPGRRVTLYVRTEGPDQRETAFRFA
                     REGNVRVFYWIDRKFGYALSSADLEREELLRLANEIYRQLNP"
     gene            complement(2341..2838)
                     /locus_tag="AC16O9_00370"
     CDS             complement(2341..2838)
                     /locus_tag="AC16O9_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6515105.1"
                     /translation="MSDVVTLIALIPRLRRYARALVGERAAADDLVQDTLERAWSKLH
                     LYRHGTDLRAWLFTVMHNVHVNQVRAARPTAALEDDMPELAQRAVQGDALLVRDLERA
                     IAALPAAQREVLLLVALEDLSYDETARVLGIPIGTVMSRLARAREKVRALMQGKGGSK
                     LQVVK"
     gene            complement(2890..3732)
                     /locus_tag="AC16O9_00375"
     CDS             complement(2890..3732)
                     /locus_tag="AC16O9_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR6515106.1"
                     /translation="MLNFGACVAPAQAQSQARGDATRGAYLAKAAGCKGCHTEAKEGA
                     TPYAGGRELKTPFGTFYGPNITPHPRAGIGKWSEADFVRALRQGERPDGKHYYPAFPY
                     PSFTQITDADLRDLWAYFRSLPASDRASRAHELRFPFGWRFLLGAWKTLYFESGPMPA
                     AHARSPQQRRGAYLANALGHCGECHTPRNWLGGPDRRRAFAGAKLADGAGAPNITPAK
                     LKKWNDAELSDFLKFGLTPDGDVVGATMGEVIRNTTSQLSQEDLAALFAYLRSLPPIA
                     EPPG"
     gene            complement(3788..4246)
                     /locus_tag="AC16O9_00380"
     CDS             complement(3788..4246)
                     /locus_tag="AC16O9_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515107.1"
                     /translation="MMKRLVTVATGAVLFAMLAPGAFAQAKPEVLVKQRQAVMTLQGK
                     YFGPMAAMAQGKAPFNAAVVQRNAGFLDNLSRMSWDGFDASTRGEKSRALPAVFDNSA
                     AFKETAARLENEAAKLVAVSRSGDEAAVKAQIGAVGKTCGGCHDEFRQKQ"
     gene            complement(4312..4947)
                     /locus_tag="AC16O9_00385"
     CDS             complement(4312..4947)
                     /locus_tag="AC16O9_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515108.1"
                     /translation="MNRMLAALAAAGLMWAAPAVAQDAAKPDLAKGEQLAKQVCAACH
                     GADGNSVAPANPKLAGQHAGYLYKQLANFKAQGDRRAARENVLMAGMVAPLSDADMRA
                     LAAYYPSQKLKPAAASDKDLAALGQKIWRGGIAERSVAACAGCHGPDGAGIPGQYPRL
                     AGQFAEYVDAQLKLFRAGGRANDPSGMMRGVAARMSDEEIKAVAEYAAGLR"
     gene            5002..5619
                     /gene="yihA"
                     /locus_tag="AC16O9_00390"
     CDS             5002..5619
                     /gene="yihA"
                     /locus_tag="AC16O9_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR6515109.1"
                     /translation="MPESSSAFSRVEFLVSAGMSGGLPPPDVPELAFAGRSNVGKSSA
                     INALARRRRLAYFSKTPGRTQTINFFDLGGRGRLVDLPGYGYARVPQDVRAQWDRLVG
                     GYLESRDALAGVVVLMDARRPFTAPDEHLLAWLRPAGRRLLVLLAKCDKLPRRARDVV
                     LSGALARLASTDPGAQALLFSSQSGEGVQEARALLEDWLRAARAE"
     gene            5786..6790
                     /gene="hemB"
                     /locus_tag="AC16O9_00395"
     CDS             5786..6790
                     /gene="hemB"
                     /locus_tag="AC16O9_00395"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR6515110.1"
                     /translation="MNIYGQFPAVRMRRMRRDDFSRRLMRETVLSANDLIYPVFILEG
                     RNRTEKVVSMPGVERMTLDRLLPVAEQCVKLRIPVLALFPVIEQKLKTLDGREAFNAK
                     GLVPRVVAALKRRFPELGVMTDVALDPYTSHGQDGVVDKSGYIVNDVTLAVLEKQALC
                     QARAGVDIVAPSDMMDGRIGRIRAALEKAGHIHTKILAYAAKYASGFYGPFRDAVGSA
                     KNLGKGDKKTYQMDPANSDEALWEVGLDLAEGADMVMVKPGLPYLDIVRRVKDEFKAP
                     TYVYQVSGEYAMLRGAIDRGWLPPSCVLEALTAMKRAGADGILTYFALEAARELKKTA
                     "
     gene            complement(6787..7614)
                     /locus_tag="AC16O9_00400"
     CDS             complement(6787..7614)
                     /locus_tag="AC16O9_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6515111.1"
                     /translation="MRRFVQGLDHAVILVRDLDAAQRAYARMGFSLTPRGTHSLGSQN
                     HCIMFEHDYLELMALPRPHPALQYFSEFLARGEGLGALVLATEDAAGLYAGLAADGIA
                     AEPPLDFSRHVADLGEARFRLVQLAVDAVPGCRMLACQHFTRDLVWRPEHQRHAVGAL
                     AIAAIAVVVEDTESAAARYARVLGARPQRIDEGLRVATGSAPIALASRWKLGHRLEGV
                     GLPPRPRPLVAALFVRVADRAQAAKVLRGNGLKPVALADGSLAVNAEDAHGVAVVFG"
     gene            complement(7620..9380)
                     /gene="dsbD"
                     /locus_tag="AC16O9_00405"
     CDS             complement(7620..9380)
                     /gene="dsbD"
                     /locus_tag="AC16O9_00405"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIR6515112.1"
                     /translation="MSRFLLLCLALAAPLAAAAEELLEPEKAFRFSARALGEDAVEVS
                     FAIADGYYMYRERFKFEAQNARLGPAQLPGGLRKKDEFFGDTEVYRNAVHIRVPVAAG
                     TADPLQLVVTSQGCADVGVCYVPMESRATLLLAAAPSTPAFSEAPRRVPETPRWSIYA
                     SDRDIAALFDRHVLAVIAGFFGLGLLLTFTPCVLPMVPILSSIIAGEGRNLNKRRAFT
                     LSASYVLGMALAYAAAGIAAAYSGTLIAAALQNPWVLGTFAAVFVWLALSMFGLYEIA
                     LPGFLHHRLSSAHQRLRGGRIASVAAMGVLSAVIVSPCVAAPLAGALLYISQTRDVAF
                     GGAALFAMALGMGVPLMIVGVSEGALLPKAGPWMNGVKKFFGVLLLAVAIWIASPVLP
                     SAAVMLAWAALLVGTGVYLHAVDRLPAPASGPVRIAKAAGIFALVAGIALLIGALSGS
                     RDPLRPLAGLTGAAPEAPIAFQRVNSSAELDAALRAPGKPVMLDFYADWCVSCKEMEA
                     FTFSDPRVRQAFGRMLLLQADVTANNDEHKALLKRFSLFGPPGIIFFDAQGREIRGLR
                     VVGYQDAERFLKTLEIAQAP"
     gene            complement(9377..9700)
                     /gene="cutA"
                     /locus_tag="AC16O9_00410"
     CDS             complement(9377..9700)
                     /gene="cutA"
                     /locus_tag="AC16O9_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR6515113.1"
                     /translation="MSALLVLTNLPDRAAAEKLADALVAKRVAACINILAPCRSVYRW
                     RGAVQHDEEHPVLIKTTRDAYAALEAEIRALHPYELPEIVAVPVERGLPAYLDWVASE
                     TTPTP"
     gene            complement(9697..10524)
                     /locus_tag="AC16O9_00415"
     CDS             complement(9697..10524)
                     /locus_tag="AC16O9_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6515114.1"
                     /translation="MKKLLIAGYGDIARRAAPQLEARYAVQALSRHHGIDLDRPDTLS
                     GLAADAVLHCAPPPATGSADPRTRNLLAALAKARILPARVVYVSTSGVYGDCGGARVD
                     EARAVQPQTDRARRRVDAERQLALWCSARGATLVILRAPGIYAADRLPLERLRRGTPV
                     LRAEDDAYTNHIHAEDLAAIAVRALEDSAPGGTYNASDDSEIRMGDWLDLVAERAGLP
                     RPPRIARSDAPGRISPELLSFMSESRRLVNRRMKEDLGVQLRYRTVFEGVPPARQAA"
     gene            10543..11565
                     /locus_tag="AC16O9_00420"
     CDS             10543..11565
                     /locus_tag="AC16O9_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HIR6515115.1"
                     /translation="MHSVTIQPSGQHFQVEDGETVLAAALRQGLVLPYGCKNGACGTC
                     KGRIVSGTVDYGAYQAKALPDEDKARGKALFCQAKPLSDLVVEARTIGAAKGIEVKML
                     PCRVQKLERLADDVMALHLKLPANEKLVFLAGQFIEFLLKDGTRRSFSMANAPHDAEL
                     LQLHVRHVVGGQFTDHVFGKMKERDILRFEGPLGTFFLREDSAKPIVFVASGTGFAPI
                     KSIIEAAVHKGVSRPMTLYWGGRRPKDLYMNALAEGWAAQGLLKYVPVISEALPEDGW
                     SGRTGFVHRAVMEDLPDLSGHQVYACGVPVMVDAARTDFTGSCGLPEDEFYADSFTTQ
                     ADAPAH"
     gene            complement(11627..12730)
                     /locus_tag="AC16O9_00425"
     CDS             complement(11627..12730)
                     /locus_tag="AC16O9_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6515116.1"
                     /translation="MKTHGILKFILALALASFGSAAWSQIVIATAGPMTGQYASFGEQ
                     MRRGAEMAVADINAKGGVMGKKLVLKIGDDACDPKQAVAVANQFVSDKVVFVAGHFCS
                     GSSIPASKVYTEEGILQMSPASTNPKLTDEGGPNVFRVCGRDDQQGTVAGNYLADKFK
                     GKKIGIIHDKTAYGKGLADETKKQLNKRGVKEAMYEAYTAGEKDYSALVSKMKGENVD
                     VVYVGGYHTEAGLIVRQAREQGYKVQLVSGDALVTDEYWKITGAAGEGTLMTFSPDPR
                     KNPAAEGVVKKFRAQKYEPEGYTLYTYGAIQAWAAAVGQAKSTKWKEVAGALRKGKFS
                     TVLGTIGFDKKGDVTAPGYVFYVWKDGKYDYAK"
     gene            complement(12779..13132)
                     /locus_tag="AC16O9_00430"
     CDS             complement(12779..13132)
                     /locus_tag="AC16O9_00430"
                     /inference="COORDINATES: protein motif:HMM:NF045145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6867 family protein"
                     /protein_id="HIR6515117.1"
                     /translation="MEALLGTGLGVYFGLTVCLMGFAAFMTGQAIAITWKPAWHALGY
                     TLLLGLADRFLAFALFDGPLLSLAAYLVDTATLAGIALLAWRLARARKMVSQYPWLYE
                     RTGPFSWRSRTPPAG"
     gene            complement(13132..13842)
                     /locus_tag="AC16O9_00435"
     CDS             complement(13132..13842)
                     /locus_tag="AC16O9_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515118.1"
                     /translation="MLRVTDLHAAYGPIKALHGINVDVAEGEIVTLIGANGAGKSTLL
                     MSICGNPRAAQGRIDFLGEDITRLPTHEIVRRGVAQAPEGRRLFPFMSVVENLQMGAV
                     SADPRHFDEDLERVFTLYPILRQRREQRGGTLSGGEQQMLAIARALMSRPKLLLLDEP
                     SLGLAPLLVKQIFSVIREINTRDGVTVFLVEQNAHHALRIAHRGYVMANGRIILSGTG
                     GELLANAEVRRAYLEGGR"
     gene            complement(13845..14660)
                     /locus_tag="AC16O9_00440"
     CDS             complement(13845..14660)
                     /locus_tag="AC16O9_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515119.1"
                     /translation="MTPLLSVEHLTMRFGGLVAVDDVSFDAPAGKITAVIGPNGAGKT
                     TVFNCLTGFYKPTAGCMRLRHPRRGEFLLERMEGYDIPARAGVARTFQNIRLFTGMSV
                     LENLLIAQHNALMRASGYTMGALFGIGGYAAAEQRAIERARYWLERVQLVDRADANAG
                     SLPYGAQRRLEIVRAMCTEPVMLCLDEPAAGLNPRESAQLNELLRFIRDEHRVTLLLI
                     EHDMSVVMEISDHVVVLDYGRKIAEGTPEVVRNDPKVIAAYLGVEDAEEAARP"
     gene            complement(14657..16129)
                     /gene="livM"
                     /locus_tag="AC16O9_00445"
     CDS             complement(14657..16129)
                     /gene="livM"
                     /locus_tag="AC16O9_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HIR6515120.1"
                     /translation="MRAAAAPRSPFDWPGALKDAGMVAGVVVLLTLALVGARIQDTSG
                     GPPLAYRFDDVVAAAVIAFFGRLAIGALRAQRPVPVLAGTLVFALALTGILLFGAEQQ
                     LLIFRSTVLNATIALAAWLLSARAAWLVWHAASETSAEVREARMDRLGAEVQKWAGWV
                     GPLLLGLAVALPFLPGIDRRMLDIATLVATYIMLGWGLNIVVGLAGLLDLGYVAFYAV
                     GAYSYALLALNFDLGFWVCLPFAGIMAAFAGILLGFPVLRLRGDYLAIVTLGFGEIIR
                     IILLNWYQFTKGPDGLSGIPRPTFFGLPMTREAPDGGATFHSFFGLEYTPLDRVIFLY
                     YLAVALALVTNLFTLRIRKLPIGRAWEALREDEIACRSLGVHPRNTKLTAFAIGAMFG
                     GFGGSFFATRQGFVSPESFTFIESAIILAIVVLGGMGSQIGVVLAAILLIGLPEVFRE
                     LNQYRMLAFGLAMVAIMTWRPRGLLAHREPTVRLGHGPPK"
     gene            complement(16129..17043)
                     /locus_tag="AC16O9_00450"
     CDS             complement(16129..17043)
                     /locus_tag="AC16O9_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6515121.1"
                     /translation="MGYFLQQLINGLTLGMIYGLIAIGYTMVYGIIGMINFAHGEIFM
                     IGAFIAFIAFLALGLIGIGWIPLALVIVLVATMLLTSVYGWALERVAYRPLRHSPRLA
                     ALITAIGMSIFLQNYVQLLQGARVKPLQPVVTGGLRFFETGEFAVAFSFLQMLIILIT
                     LGLMVGFTLIITRTALGRAQRACQQDLGMAALVGINVDRTIAITFVMGASLASVAGLM
                     FLLNYGVIDFYIGFLAGIKAFTAAVLGGIGSVPGAMLGGLLIGLIEAFWSAYFSVEYK
                     DVATFAILILVLIFRPTGLLGKPDVEKV"
     gene            complement(17119..19104)
                     /locus_tag="AC16O9_00455"
     CDS             complement(17119..19104)
                     /locus_tag="AC16O9_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6515122.1"
                     /translation="MSNRLAGETSPYLQQHAANPVDWQPWDAAALARAQREDKPILLS
                     IGYSACHWCHVMAHESFEDPEVAAVMNRHFVNIKVDREERPDLDQIYQTAHQMLAQRG
                     GGWPLTMFLAPDGTPFWGGTYFPKRARYGLPGFVEILERIAQVWREQRAAVGEQGEQV
                     RLALERLQPRTPAGGEALDAEPVRAALDVLRASFDARNGGFGGAPKFPHPTDLELCLR
                     RGETAIVDTTLRRMCEGGIFDQLRGGFCRYSVDAEWRIPHFEKMLYDNGPLLALLADA
                     WRVTNDDLYARCAAATAGWLMHEMQSPEGGYTSSLDADSEHEEGKYYVWDADQARALL
                     TSEEWAVTAPHFGLDGPPNFEGRHWHLRVARPLRTGGEALLAAAKAKLLAARGKRVRP
                     GRDEKVLVSWNALAIGGMARAGRVFGEQAWLASARRALDFVRSRMWQGGRLLATYKDG
                     RAHLNAYLDDYAFLMLALVELLQAQFRAEDLAFATALADVLFEQFEDRASGGFYFTAR
                     DHERLIHRPKPGHDQATPSGNGAAALALNRLAALTGETRYAVAAERTLRLFLPGVRAQ
                     PAGYATLLMALEEWLAPPATAIVRGAPEALRAWTGAMAREYLPRTLVLAIPAGLGGLP
                     PLLDKPGSAAVQAWLCRGAVCLPPFDGIDALRNACRA"
     gene            complement(19101..20045)
                     /locus_tag="AC16O9_00460"
     CDS             complement(19101..20045)
                     /locus_tag="AC16O9_00460"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HIR6515123.1"
                     /translation="MRQVATPAAAGVFISAAVLLAGCAAPQTSVIVERPPANLPVRAE
                     LTHTPFFPQEDYQCGPASLATVLQHAGRNAMPASLVPQVYVPERQGSFQAEMLAATRR
                     HGLVAYTLEPRLDTLLREIAAGTPVLVLQNLALDWAPQWHYAVAIGYDLEARELVLRS
                     GVTRRLAMRLDTFEHTWARSGHWGMLALPPARLPETVAERPYLAAVAALERVDPAAAR
                     RGYEAALARWPDSVAAQIGLGNASYALRDLSGAAAAYRRAAQRHPGSADAWNNLAQAL
                     TELGSRDDALAAARRAVALGGPRLPVYRETLEHILRQP"
     gene            complement(20053..20412)
                     /locus_tag="AC16O9_00465"
     CDS             complement(20053..20412)
                     /locus_tag="AC16O9_00465"
                     /inference="COORDINATES: protein motif:HMM:NF042483.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HIR6515124.1"
                     /translation="MVAGLIIVCLGGTGLVLPAHAGIVSSDVLVAGAQHERLAGLLER
                     SDVQARLKAYGVDPVQAQARVAALTDEEAAHLAAQIDELPAGGDVLGAAVLIFLVLLF
                     TDVMGYTKIFPFTRSVR"
     gene            20539..21081
                     /locus_tag="AC16O9_00470"
     CDS             20539..21081
                     /locus_tag="AC16O9_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR6515125.1"
                     /translation="MSDPRALEVLRFWLDEHSKDWFVKKPDLDAEIRRRFHALYLEAA
                     TGRLQDWLDDARECLALVILLDQFARNMFRGEAQAFATDARARAAARVILDHGWDKAM
                     TQAEQLFAYLPFEHSESLEDQDLSCELMKDFGAEQRRYAERHREIIARFGRFPHRNGI
                     LGRESTAAEIEFLKLPGSGF"
     gene            complement(21070..22752)
                     /gene="ilvD"
                     /locus_tag="AC16O9_00475"
     CDS             complement(21070..22752)
                     /gene="ilvD"
                     /locus_tag="AC16O9_00475"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6515126.1"
                     /translation="MADNRRSQLITQGVARSPNRAMLRAVGFKDGDFEKPIVGVANGH
                     STMNPCNAGIQPLVDSAMAALEAAGAKPQVFGVPTITDGIGMGTEAMKYSLVSREVIA
                     DAIETSVNGQAMDGALVVGGCDKNMPGGMMAIARMNVPAIYVYAGTIKPGTWKGQDLT
                     IVSAFEAVGAFSAGRMNEEDFVGIEKNACPSVGACGGMFTANTMSSSFEALGMSLLGS
                     SQMASPDREKADSAAESARVLVEAVKRNLKPRDIITRKSIENAIALVMATGGSTNAVL
                     HYLAIASAAGVKWTIDDFERVRRKVPVLCDLKPSGRYVAVDFHRAGGVPQVLKILLKN
                     GVLHGECMTIHGKTMAEMLKDVPAEPRADQDVIRPWSNPLYRQGHLAILKGNLATEGC
                     VAKITGLKKTAITGPARVFDSEPACMKAIMAKRIKAGDVVVIRYEGPKGGPGMQEMLA
                     PTSALIGQGLGDSVGLLTDGRFSGGTWGMVVGHVAPEAYVGGTIALVKEGDSVTIDAK
                     KHLVRLNVPAKELARRRKRWKAPKPRYTRGLLAKYMKLVSTASKGAITDSET"
     gene            22789..23565
                     /gene="lgt"
                     /locus_tag="AC16O9_00480"
     CDS             22789..23565
                     /gene="lgt"
                     /locus_tag="AC16O9_00480"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6515127.1"
                     /translation="MLIHPQFDPVALSLGPLAIRWYGLMYLAAFGFLWWLGRIRIARG
                     LAPIGREQFDDLIFLGVLGTILGGRLGYVLFYKPGYYLEHPLEIFAIWQGGMAFHGGF
                     LGVLAAVAFFAWRRGVDWWRLMDFIAPCVPTGLAAGRLGNFINGELWGRPTDLPWGMV
                     FRGAGSLPRHPSQLYQFALEGVLLFVLLWWYAAKPRARGAVSAMFLMGYGTFRFIAEF
                     AREPDEFLGLLALNLSMGQWLSLPMIAAGAALYVWSRGQK"
     gene            23562..24020
                     /locus_tag="AC16O9_00485"
     CDS             23562..24020
                     /locus_tag="AC16O9_00485"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6515128.1"
                     /translation="MRWLLLALALACTALAARAQSHEVPSWFAESFLEFPQDVKEAAG
                     DGKRLMLYFWQEGCPACRKMKETTLADPALVEATRRRFVAVALNVFGERELQWTDGRT
                     LREKELARALGIRGTPTLLFLDERGAVVLRRVGYLPPEGFAAALAEAKGR"
     gene            complement(24017..26890)
                     /locus_tag="AC16O9_00490"
     CDS             complement(24017..26890)
                     /locus_tag="AC16O9_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR6515129.1"
                     /translation="MDAQARPVRIVGPRTGEASIAARLRKAVDGEVLFDAASRGRYST
                     DASIYQVEPVGVVVPRTEEAARAAIAIAVEAGVPVLPRGAGSSQCGQAVGAALVIDDS
                     KYLNRVLEVDAAGRTALVQPGLVLDQLNAQLRAHKLWYPVDVSTSAQATIGGMAGNNS
                     CGSRSIAYGNMVHNVLAIDAVMAGGERWRFGPMRDASGPPGYLELVRKLGALYEREKD
                     EIAARFPKVLRRVAGYNLDHLGPPHANAAHLLVGSEGTLAYSERLLLRLAPLPPARAL
                     GVCHFPKFYTAMDLTQHIVKLGPSAVELVDRTMIGLARDIGAFRRTVDAVIQGEPDAI
                     LLVEFSGDDAPAQVSKLKQLSQLMGDLGLPGSVIEITDAGLQKDVWEVRKAGLNIMMS
                     MKGDGKPVSFIEDCAVPLEHLAEYTERLTQVFQKHGTRGTWYAHASVGTLHVRPVLDM
                     RRDGAEKMRAIAEEACALVKQYKGAYSGEHGDGLVRSEWIAPFFGPRLTACLAEIKSW
                     LDPQGLMNPGKIVAPGRMDDKALFRFKPGYATQVPSTALDWGEWGGYDKAVEMCNNNG
                     HCRKFDAGTMCPSFRATGDEMHLTRGRANTLRLALSGQLGASADEDVKAALDLCVSCK
                     GCKRECPTGVDMARMKIEFLAHYRRRHGATLRERLIAYLPRYAPWAARAAGLANLGAR
                     ATRTLLGFTPRRELPPWRSDYFVDRVWPKRGRREVVLLVDTFSRYFEPENARAAVRVL
                     EAADYRVHVAQPASGERPLCCGRTFLTAGMLDQARVEARRVLEALRPWLGLGVPVIGL
                     EPSCLYTLRDEYPVLLPEARAQAGSTYLFEEFLAAELDAGRFAPALRPLAAQALVHGH
                     CHQKAFGAMGAVERVLRTVPALQVSIIESSCCGMAGSFGYEAEHYEASMKMGERSLLP
                     AVRNAPAEALIVADGTSCRHQIADGAGRRAEHVARVLARALES"
     gene            26953..28146
                     /locus_tag="AC16O9_00495"
     CDS             26953..28146
                     /locus_tag="AC16O9_00495"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR6515130.1"
                     /translation="MPRNAGRHFLQIPGPTNVPDRVLRAIDFPTMDHRGPEFADLGKA
                     VLEGLRRVFKTRGHVVIYPASGTGAWEAALVNTLSPGDAVLMCETGHFATLWQKMAVK
                     LGLKPEFLPGDWRSGADPQAIEKRLRADTANNIKAVCVVHNETSTGVASRIAEVRKAI
                     DAAGHPALFMVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNAISDKALAA
                     AKSAGLPRSYWAWEEMLASGKSGYFPYTPATNLLYGLREALAMLEEEGLDNVFARHQR
                     HAEATRRAVRAWGLEVLATNPAEYSASLTAVLLPAGHDADRVRKVVLEAFDMSLGTGL
                     GKLAGKVFRVGHLGDFNDLALTGTLAGVEMGLTLAGVPVKNEGVAAAMRYLAECHRPK
                     ARAAA"
     gene            28176..29147
                     /locus_tag="AC16O9_00500"
     CDS             28176..29147
                     /locus_tag="AC16O9_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6515131.1"
                     /translation="MKLRASLAALLLAALAAPAAAQTELRFGHVGEPGSLIAASAEEF
                     ARIANAKLAGKAKVVVFGSSQLGNDRELLQKVKLGTVDFALPTTVMSSEVDLFGLFEM
                     PYLVKDRAHMARIEKEIFWPSLAPLAEQKGLKVLAVWENGYRHITNNKRPIVKPADLN
                     GVKLRTPRGKWRVKMFEAYGASPSPLPFSEVFTALQTGVMDGQENPFANIWTAKFYEV
                     QKYLSLTGHVYTPGYPVVGINKWKTHSPELRKTLEDAAKETQAYVYSTAISMENDLLG
                     KLKSGGIKVNEADKDAFIAGSKAIYEEFGKEVPGAQALIDKAIALGK"
     gene            29227..29718
                     /locus_tag="AC16O9_00505"
     CDS             29227..29718
                     /locus_tag="AC16O9_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6515132.1"
                     /translation="MADRLRTAFERLLEVVVFVLMIALALLVAAAVVFRKFNAPIIWY
                     DEVAVILLAWITYYGSALAALKGAHISVSAVVDALPRGPRIVVTLFGEACVIAFFLVL
                     GWAGYQVLGVLATDYLVTLPKVSNDYVQSIIPIGSACFVIAELLRLPAVLRGAAARSE
                     ASP"
     gene            29720..31000
                     /locus_tag="AC16O9_00510"
     CDS             29720..31000
                     /locus_tag="AC16O9_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6515133.1"
                     /translation="MAVVLLFLGVLALVLINVPIAVALGIAAAVAMIAAQGAGALPNM
                     AIVMYSGTTGFPLIAIPLFIFAGAIMNTSGISRRLIAFASAILGFIRGGLAMVNIGSS
                     MFFAEISGSAVADVAALGSVLMPAMKKKGYPGTFAAAITSSSASLAIIIPPSIPMILY
                     AVMSGASVVQLFVAGIIPGIIGGLAMMALSYWYAVRFNWPVEEVFQLKRVWKTFKDAA
                     LALLLPVIILGGIFGGIVTASEGAGLAVVAAIGIALYYREIQWQQLWTAVIDGAVQTA
                     AVMLLVAASALLGVYLTEAQVPQKLAQVIVGLTKEPWAVLLLLNFFFLFIGLFLHSAA
                     AIILVVPIVMPVVNAVGIDPVHFGLIVTLNLAIGQQTPPVASVLMTACSIAKENVWDV
                     TKVNLPFIGVLVSVLLLITYVPAIPLALVNLFYR"
     gene            31008..31787
                     /locus_tag="AC16O9_00515"
     CDS             31008..31787
                     /locus_tag="AC16O9_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6515134.1"
                     /translation="MPESVYVERDGAIATVVLNRPDKMNATTVDMWLRLGDAFRALNA
                     DEALRCIVLRGAGRKAFAAGADIGEFGKTRSDAKAAKAYSRQVGGTMHEIAHARHPTL
                     ALIHGACVGGGLELAALCDLRIAGESARFGVPIKKLGLVVAYDEMAALIALVGRATTL
                     EILLEGRVFGAAEALAKGLVTRVVADDRLDEEAYAAARRIAEGAPLAARWHKKFARRL
                     ADPRPLTEEEYDESYACFETEDFKEGAAAFLAKRAPQFRGR"
     gene            31705..32982
                     /locus_tag="AC16O9_00520"
     CDS             31705..32982
                     /locus_tag="AC16O9_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6515135.1"
                     /translation="MPASKPRTSRKAPRRSSPSARPSSAAAKVGPLAGVKVLELAQIM
                     AGPTCGMLLADLGADVIKVERIPGGDDTRSMDRHSINGESAAFMAMNRNKRGIALNLK
                     LPAAQEALKRMAAKADVLTENYRKGTLEKLGVGYETLKKINPGIIFCSISGYGRTGPY
                     ADKGGYDLIAQGMSGLMAITGEPGRAPVKVGSPVCDINGGMLAALGVVSAYVHRLKTG
                     KGQMVDTSLFEAGIQQTYWQAAMHFATGESPGPGGSAHPLSAPYQAFQAKDGWLTIGG
                     ANQANWQRMARVLGAPEWIEDPRFLNNTQRMNNLPALVALMNEKLAAKTVSEWLALLD
                     AAGVPCGPINSIAQVFQDPHAIAREMVVELEHPKAGHTRAIGLPIKLSASPGKVRRPA
                     PLFGQHTREVLAEFGFGKKEIEDLIGSGAAIAA"
     gene            32985..34130
                     /locus_tag="AC16O9_00525"
     CDS             32985..34130
                     /locus_tag="AC16O9_00525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6515136.1"
                     /translation="MDFTLSEEQRLFRDSVRGFAAKHLAAGALARAHDPAHPFDVARL
                     MAKQGLLGITIAEADGGQGGTLMDAVLAIEAVASVCPRSADVVQAGNFGPVRVLAEFG
                     SPEQKKKYLAPILAGETVMCLGMSEPEAGSAVTDLKTSATPDGDGYRVTGNKVFSTHG
                     PYASVILAYVRYGLGVGGIGSVLIEPGWKGVTLGKPSRFMSGEEWVEIHFEDVRVPRE
                     NVLLGPGGFKKQIAGFNVERIGNTARSLALGRYAYEAAREWALTRHQFGRALAEFQGL
                     QWKFADMKMKLDAGELLLYRAAANADRGMPSAEETAIAKAFCNQAGFEVASEALQVMG
                     GTGYSQELLVEYCLRRCRGWMIAGGSIEMMKNRIAEGIFGRSFPQRP"
     gene            34127..36142
                     /locus_tag="AC16O9_00530"
     CDS             34127..36142
                     /locus_tag="AC16O9_00530"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR6515137.1"
                     /translation="MSGELFRALFAPRGIALYGASGDPSKNTARPQRFLRKHGYAGSI
                     VPINRTRREVLGETAYPSLAAAPGDIDQAFVMVPPADVPAACAECFARRVPVVTIFTD
                     GFGETGDAGRQAEARLVAAAKSAGTRLLGPNSIGLVSTSPPAPLTVNATLELERLPVG
                     PLAVVSQSGSLIGALLSRGAARGIGFSRLVSVGNEADLSVGELMDLLVDDAQTGVILL
                     FLETLRDAKRIERAARRAFDAGKPVIAYKLGRSAAGAALARSHTGALAGDDRAADAFF
                     GACGILRVRSFEALIETAQLAIGQRPPRGKRVAALTTTGGGAAVVADPLGAAGAELVA
                     PPPALADTIRALGVRVADSPLIDLTLAGTRANVYGPVLEALLASDHCDAVLAAVGSSA
                     QFHPQLAIEPILEAHRKHSGKPLAVFLAPEAPASLQLLAQAGIAAFRTPESCADALAA
                     FLSWRAPRAPASPVSFDIPQAPTQRLRTPDEPITIPFPVAIKIDSPDIEHKTEVGGVE
                     LDVRDAKEAAAAARRVLERVRGACPRARVNGVTVSSMQTGLAEAILGYRLDPQVGPLV
                     LVGAGGTLAELYRDFALRLAPVSLEEAREMVAEVRGFAPLRGYRNAPRGDLEALARAV
                     AAHSRLAARPEIAEAEINPLIVKADGVVGVDALVVLREGAPDHGAAA"
     gene            36126..36497
                     /locus_tag="AC16O9_00535"
     CDS             36126..36497
                     /locus_tag="AC16O9_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515138.1"
                     /translation="MALPLERLMERAAGDALLARFGLDFDCALLVGDGDTDWLVEIAR
                     GAIVRVAKGPFIMPKTDLRLRAASEVWGKFLAPVPPPGYHDLLALRRYRRIQIEGDIR
                     LMSAYLFYLKRLFALLRAPGA"
     gene            36494..37348
                     /locus_tag="AC16O9_00540"
     CDS             36494..37348
                     /locus_tag="AC16O9_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6515139.1"
                     /translation="MKAAREPVTGYYLNVEILGRPQRLYVEEAGQGIPLLCLHTAGSD
                     TRQYRGLMNDAGILRDYRVIAFDMPWHGKSSPPAGWQTEEYRLTSADYARMVLEVCDA
                     LALERPAVMGCSIGGRIALVLAMEHAKRFRAIIGLQSGASIDPYYELTWFHHPQVHGG
                     EAAAAYVSGLMAPMSPDTDRWETLWHYMQGGPGIFKGDLYFYRGADGDIRERLSAIDT
                     RVCPLYLLTGEYDYSVSPEDTRAAAERIPGVKFTVMPKLGHFPMSENPALFLSYLKPV
                     LDEIRARG"
     gene            37354..38712
                     /locus_tag="AC16O9_00545"
     CDS             37354..38712
                     /locus_tag="AC16O9_00545"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR6515140.1"
                     /translation="MRAVERCGEFVEFMREAPLAAEVLHHAKRAVIDWVAAALPGAGV
                     PPALALEAALVEELDRGAAQLVLGRPATVRAAALINGTAAHTAEVDDIFRDGIYHPGA
                     PTIAAALALAQAGSLSGLRFLRAVIAGYEVSTRIARAMGRAHYRFWHNTGTIGAFGAA
                     AAAAGALSLDRKRSAHALATVATFAAGLQQAFRSDSMSKPLHAGRAAEAGVTAALAAR
                     EGVTGSLDVLDGAAGFGRAMGEGPDWVDAFATLGTHYNIARMTFKNHACCGHTFAAID
                     GALAARAALGAAAPDIARVRIGSYRAATEVSGIENPRTAAEARFSIKYVVAHALRHGS
                     VRLAAFEAAPLADPETRALMGCIEAAVDPELDAAFPGQRAARVAIETRDGRSHALLQP
                     ARKGDPELPLDDAELEAKYLELAAPVIGAAPARALLARLWRLEREPGVSLAAAAPPRA
                     AR"
     gene            complement(38672..39823)
                     /locus_tag="AC16O9_00550"
     CDS             complement(38672..39823)
                     /locus_tag="AC16O9_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515141.1"
                     /translation="MSKRTLTTCSAVHSLHDGLSDVTYVLLPLLANAFGLSLAQVGII
                     RGAHRAAMAAFQIPAGILAERVGERGLLAFGTALAGAAFIALGYAQGFWAILAALFFA
                     GMGSAVQHPLASTLVSRAYPVQGRRAALGTYNFAGDVGKFAFGGLVSLLLLAGVAWQA
                     PVIGFGVAAIAVAVAVLALLAAGRAPAPQHASRGDRAMDWGIRDRPRFLALCAIEVLD
                     ASTRSAFLTFIAFLLLAKGLSAGWAVAAVPLVLAGGMAGKLACGVLAERIGVPRMIVA
                     TEIATAAGIFAVLALPAAGALLLLPLLGVVVQGTSSVLYATIGEYVRPERLSRAFGLF
                     YTVGSVCGIVAPLTFGLLGDQLGVQAAIAALGGTILLTLPLVAVLRPRG"
     gene            39854..40891
                     /locus_tag="AC16O9_00555"
     CDS             39854..40891
                     /locus_tag="AC16O9_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515142.1"
                     /translation="MVDCPARGHPTRRGKVAKLLEVKNLQTQFLTSAGVVRAVDGVSY
                     DINEGETVALVGESGCGKSVSALSIMRLVAEPAGRIVGGEILFKGRDLLKLTEEEMRD
                     VRGRDITMIFQEPMTSLNPVLSIGRQLTEGLEIHLGMNQKAAQARAVELLGLVGIPDP
                     QRRLTQYPHQFSGGMRQRMMVAMALACNPKLILADEPTTALDVTIQAQILELLQDLSR
                     RLGVAMLIITHNLGVVARYADRVNVMYAGRVIEQGSAAEIYADPRHPYTLGLLRSVPR
                     LDEPMRAKLDPIEGQPPDLTRLPPGCAFAPRCAFRVDRCTETPPLTALGAPGHLSACW
                     EAEHLAKEVPA"
     gene            40888..>41215
                     /locus_tag="AC16O9_00560"
     CDS             40888..>41215
                     /locus_tag="AC16O9_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6515143.1"
                     /translation="MSQPLLEVHGLVMHFPVGGGMFSKPAGVVRAIDGVSFNIRKGET
                     LGLVGESGCGKTTTGRCILQLERPTGGRIVFEGVDMMALDAAELRAVRRRVQVIFQDP
                     YSSLKPR"
BASE COUNT         6564 a        14260 c        14111 g         6280 t
ORIGIN      
        1 gccgagcggc tctaccgcgc ggcggcacag cgcgcgcatc tcgagccggt cggcatcggc
       61 tgccacatcg ggtcgctgct cgcggatccc gcgccatgga tcgcggcagc cgagcgcgtg
      121 atcgagctgg ccgagcgcat cacgcaagcc ggcatccccc ttcagcacgt cgacgtgggc
      181 ggcggcatcg gcatccgcta ccgggacgag ccgcagcgca ccatcgaaga gttcctgagc
      241 ggcgcgctgc gcgcgctcgg cgagcgcgat gagatgttga tcgtcgatcc gggccgctcg
      301 atcgtcggca acgccggcgt gctgctcacg cgggtggagt atgtgaagcc cggcagcgcc
      361 aagaacttcg tggtggtgga tgcagccatg aacgacctga tgcggccggc gctctacgac
      421 gcgtggcacg aggtgcgcca ggtgcgcgaa ccgcagcccg gcgcggccac cgccgtctac
      481 gacgtcgtcg ggccgatctg cgagagcgcc gattttctcg cccgggatcg aacgctcgtg
      541 gcgcacgccg gcgaactgct cgcgctgatg tcggcagggg cttacggcat gagcatgagc
      601 tcgaactaca attcacggcc gcgctccgcc gaggtcatgg tggacggcgc gagcgcccac
      661 ctcgtgcgcc gccgcgaaga ggtgcgcgcg ctgtttgcgg gcgagagcat cccgacatgg
      721 tgaaatcggc acaacgacaa gggcaatcat gactgagacg cgcaacctcg ccgtattctg
      781 cgacttcgag aacatcgccc tcggcgtgcg cgacgccaag tacgagaagt tcgacatcgg
      841 ccgcgtgctg gagaagctcc tgctgaaggg ttcgatcgtg gtgaagaagg cctactgcga
      901 ctgggatcgc tacaaggagt tcaagtcgac gatgcacgcc gcggcgttcg agctgatcga
      961 gatcccgcac cgcagccagt ccggcaagaa ctcggccgac atccgcatgg tcgtggacgc
     1021 gctggatctg tgctacacca aggagcacgt cgacaccttc gtcgtggtca gcggcgattc
     1081 cgacttctca ccgctggtgt ccaagctgcg cgagaacgcc aagaacgtga tcggcgtcgg
     1141 cgtgaagaac tcctcctccg acctgctgat ctccaactgc gacgagttca tctactacga
     1201 cgacctggtg cgcgaggacg aggccaagcg ccgggcagcg aagaagcgcc gcgaggcgcg
     1261 cccggccggc gcggcgaagg gagtagcgct aagcgatgaa aagaagcagg aggccttcga
     1321 cctggtgatc gagacgctgc aggcgctgat cgccgagcgc ggcgagggcg aacggatctg
     1381 gggctcaatg gtgaagcagg cgctgaagcg caggaaaccc ggcttcaacg agtcctacta
     1441 cggcttcaag gccttttccg acctgctcga ggagctgcag aaccgcaagc tgctcgccct
     1501 cgagcgcgac gacaaatccg gcggctacct gatccgcccc gcgagcggcg gctgaggcgc
     1561 ctcagggatt gagctggcgg tagatctcgt tggcgagccg cagcaactct tcgcgctcga
     1621 ggtccgccga agacagcgcg tagccgaact tgcggtcgat ccagtagaac acgcgcacgt
     1681 tgccttcgcg cgcgaaacgg aacgcagtct cgcgctggtc cggtccttcg gtccggacgt
     1741 aaagggtgac ccgccgcccg ggtgcgctct ggtacatgaa ttgcgccaca gggccgggat
     1801 cgcgcggcag tagccgcccg ccgaccagcg tcatgccctc cgcatcgagc cgtggggcgc
     1861 gcacctcgcc gccaaggcgc ttggacagcc aggccaccag atgctgctcc tggtcggcgc
     1921 ccacttcgac cggatggcgc acctcgggcg cgtaggttgc atgcgcgagc gcggcgcggc
     1981 gcgcaagcac tggcgtctca gtccgtgcga gcgggacggg cgcatagcgc gcgtgcagct
     2041 gccaccccgc cacgccgccg atcgtcacgc cgagcgcaag ccagcccgcg accgccgccg
     2101 cgacactcca gcgccgacgg cgcagtgccg cgtgctccag gcgctccggc accggctcgt
     2161 cgagcaccgg ctcgaaatgc gcgcgcagct gctcgttgag cgtgcgatac gccgccgcgc
     2221 gctccgcgtc ctcggggtgc gccgcaagcc aggcctcgac ctcaacgcgc cggccctcgg
     2281 ccagacagcc gtcagcccag gcgtgcaggt cggtttcgcc aatgtgattt tgcgcttcgc
     2341 tcatttcacc acttgcagct tgctgccacc ctttccttgc atcaatgcgc gcactttctc
     2401 gcgagcgcgc gccaaacgcg acatcaccgt gccgatcggg attcccagca cgcgcgctgt
     2461 ctcgtcgtag ctcagatcct cgagcgccac caacagcagc acctcgcgct gcgccgcggg
     2521 cagcgcggcg atggcgcgct ccaggtcgcg caccagcagc gcatcgccct gcaccgctcg
     2581 ctgcgccagc tcgggcatgt cgtcctcgag cgcagcggtc ggccgcgccg cgcgcacctg
     2641 gttcacgtgc acgttgtgca tgaccgtgaa cagccaagcg cgcaggtccg tcccatgccg
     2701 gtacaggtgc agcttcgacc aggctcgctc cagcgtgtcc tgcaccaaat cgtccgccgc
     2761 ggcgcgctcg ccgaccaggg cccgcgcgta gcgccgcaag cgcggtatca aagcaatcag
     2821 ggtcacgacg tcgctcatct ggcttaacag tgctctgcgg cgcactattc cgtcaagatc
     2881 ggcaagcgct caccccggcg gctcggcgat tggcggcagg ctgcgcaggt aggcgaacag
     2941 agcggcgagg tcctcctgcg acagctggct ggtcgtgttg cggatcactt cccccatggt
     3001 agcgcccacc acgtccccgt ccggcgtgag gccgaacttg aggaaatcag acagctcggc
     3061 atcgttccat ttcttcagct tggcaggcgt gatattcggc gcaccggcgc cgtccgcgag
     3121 ctttgcaccg gcgaacgcgc ggcggcggtc ggggccgccg agccagttac gcggggtatg
     3181 gcattccccg caatggccca gcgcgttcgc caggtaagcg ccgcgccgct gctgcggcga
     3241 gcgcgcgtga gccgccggca tcggtcccga ctcgaagtac agggtcttcc aggcgcccaa
     3301 gaggaagcgc cagccgaacg gaaagcgcag ctcgtgcgcg cggctggccc ggtcgctcgc
     3361 cggcagcgag cggaagtagg cccacagatc gcgcaaatcc gcgtccgtga tttgcgtgaa
     3421 cgaggggtag ggaaaggcag gatagtagtg cttgccatcg ggccgctcgc cctggcgcag
     3481 cgcgcgcacg aagtccgcct cgctccactt gccgatcccc gcccgcggat gcggcgtgat
     3541 attcggcccg tagaacgtgc cgaacggcgt cttcagttcg cgcccgccgg catagggcgt
     3601 cgcgccttcc tttgcctcgg tgtggcagcc tttgcagccc gcggccttgg cgaggtaggc
     3661 gccgcgcgtg gcgtcgcccc gcgcctgtga ctgtgcctgt gctggcgcga cgcatgcgcc
     3721 gaagttcagc accgccaaaa agaaaagccc cgccagggcg gggcttgtgc accgtgccaa
     3781 gcctggacta ctgcttctgc ctgaactcgt cgtggcagcc gccacacgtc ttgccgaccg
     3841 cgccgatctg tgctttcacc gcggcctcgt cgccgctcct gcttacggcg accagcttgg
     3901 ctgcctcatt ctcgagccgg gccgccgtct ccttgaacgc ggcgctattg tcaaacaccg
     3961 cgggcagggc gcggctcttt tcgccgcggg tactggcatc aaagccatcc caggacatgc
     4021 ggctcagatt gtcgagaaag cccgcgttgc gttggaccac ggcggcattg aaaggcgcct
     4081 tgccctgtgc catggcggcc attgggccga aatacttgcc ctgcagcgtc atcaccgcct
     4141 ggcgttgttt tacaagaacc tccggctttg cctgcgcgaa cgcgcccggc gccagcatcg
     4201 cgaacagcac ggcgcccgtg gcgaccgtca ccagcctctt catcatcgga atctccttaa
     4261 gttggacccg ccgggggcaa acagcccgtg ccgacgttta tttcacgcgc cttaacgcag
     4321 gcccgcggcg tactcggcga ccgccttgat ctcctcgtcg ctcatgcgcg cggcaacgcc
     4381 gcgcatcatg ccgctggggt cgttggcacg gccacccgcc cggaaaagct tgagctgcgc
     4441 atcgacgtat tccgcaaact ggccggcgag ccgcggatac tggcccggaa tgccggcgcc
     4501 gtcgggacca tggcagccgg cacaggcggc cacgctcctc tcggcgatgc cgccgcgcca
     4561 gatcttctga ccgagcgccg cgaggtcctt gtcgctcgcc gcggcgggct tgagcttctg
     4621 cgatgggtag tacgcggcca gcgcgcgcat gtcggcgtcg gagagcggcg ccaccatgcc
     4681 cgccatgagc acgttttcgc gcgccgcccg cctgtcaccc tgcgccttga agttggcaag
     4741 ctgcttgtaa aggtagccgg catgctgccc ggcgagcttc ggattggcgg gcgccacgct
     4801 gttgccgtcg gcaccgtggc aggcagcgca cacctgcttg gcgagctgct cgcccttggc
     4861 gagatccggc ttggcggcgt cttgagccac ggctggcgcg gcccacatca gccccgcggc
     4921 ggccagcgcc gccagcatgc gattcattct gctactccct gaaatcggta ggaaacgcct
     4981 cattttatta gaatttcatc catgccggaa tccagcagcg cgttctcccg cgtcgagttc
     5041 ctggtttctg cggggatgag cggcggcctg ccgccgccgg acgtacccga gttggcgttc
     5101 gccgggcgct cgaatgtcgg caagtccagc gcgatcaacg cactggcccg gcgccggcgc
     5161 ttggcatact tctccaagac gccaggacgc acgcaaacca tcaatttctt cgaccttggc
     5221 ggccgcggcc ggctggtgga tctgccaggc tacggctatg cgcgtgtgcc tcaggacgtg
     5281 cgcgcacaat gggaccggct agtggggggc tatctcgagt cgcgcgacgc gctcgcaggc
     5341 gtggtggtgc tgatggacgc acggcgcccg ttcaccgcgc ccgacgaaca cctgctggct
     5401 tggctgcgcc cggccggacg ccggcttctg gtgctgctgg cgaagtgcga caagctcccg
     5461 cgccgagccc gggacgtcgt gctgagcggt gcgcttgccc ggctcgcttc caccgacccg
     5521 ggcgcgcagg cgctgctttt ctcgagccag agcggcgaag gggtgcaaga ggcgcgcgcg
     5581 ctgctcgagg attggctgcg ggcagcgcgc gcagaataaa aaacccccgg caaaggggat
     5641 ataaaccggg ggcaaaacgc cttaattggg attaaggcaa cccgctcagg gaggagaagc
     5701 gggagacgat caatatcgtc tgcaggtaag attgccagcg gcgtggaaag ttccgcgttc
     5761 cgaaaccctc agaataatca gcgctatgaa catctatggg cagttcccgg cagtacgcat
     5821 gcggcgcatg cggcgcgacg atttctcgcg gcgcctgatg cgcgagacgg tgctcagcgc
     5881 caacgacctg atctatccgg tattcatcct cgaaggtaga aaccgcaccg agaaagtggt
     5941 ttcgatgccg ggtgtcgagc gcatgacgct ggaccggctc ctgcccgtgg ccgagcagtg
     6001 cgtaaagctg cgcatcccgg tgctcgcatt gtttccggtg atcgagcaga agctgaagac
     6061 gctcgatggg cgcgaggctt tcaatgccaa ggggctggtg ccgcgcgtgg tagccgcgct
     6121 gaagcggcgc tttccggagc tcggtgtcat gaccgacgtt gcgctcgatc cctacacttc
     6181 gcacgggcag gacggcgtcg tcgacaagtc gggctatatc gtcaacgacg taaccctcgc
     6241 cgtgctggag aaacaggccc tgtgccaggc gcgcgcgggc gtcgacatcg tcgcgccctc
     6301 ggacatgatg gacgggcgca tcggccgcat ccgcgctgcg ctcgagaagg ccggccacat
     6361 tcacaccaag atcctcgcct acgcggccaa gtacgcttcg ggcttctacg gcccgttccg
     6421 cgatgcggtg ggttcggcga aaaacctcgg caagggcgac aagaagacct accagatgga
     6481 cccggcgaat tccgacgaag cgctgtggga ggtcgggctc gatctcgccg aaggcgccga
     6541 catggtgatg gtgaagccgg gcctgcccta cctggacatc gtgcggcgcg tgaaggacga
     6601 gttcaaggcg ccgacctacg tctaccaggt ctcgggcgag tacgcgatgc tgcgtggcgc
     6661 gatcgaccgc ggctggttgc cgccgagctg cgtgctggaa gccctcaccg ccatgaagcg
     6721 cgccggcgcc gacggcatcc tcacctactt cgcgctcgag gcggcgcgcg agctgaagaa
     6781 gacggcctag ccgaacacga ccgcgacgcc gtgcgcgtct tcagcgttca cggcgagcga
     6841 cccatctgcg agcgctaccg gcttgaggcc gttaccgcgc aacactttcg ccgcctgcgc
     6901 acgatccgca acgcggacaa agagcgcggc caccagcggc cgcgggcgcg gcggcaggcc
     6961 tacgccttcg aggcggtggc ccagcttcca gcgcgaggcg agcgcgatcg gcgcgctgcc
     7021 ggtcgccacc cgcaagccct cgtcgatgcg ctgcggacgc gcgccgagca ctcgcgcata
     7081 acgcgccgcc gcgctctcgg tatcctcgac gacgacggct atcgccgcga tcgccagcgc
     7141 gcccacggcg tggcgctgat gctccggccg ccataccagg tcgcgcgtaa agtgctggca
     7201 ggcgagcatg cggcatccgg gcaccgcgtc cacggcgagc tgcacgagcc gaaagcgcgc
     7261 ctcgcccagg tccgccacgt gtcgcgagaa atctaggggg ggttccgccg cgatcccgtc
     7321 tgccgcgagc cccgcgtaga gcccggccgc gtcctcggtg gcgaggacga gggcgccgag
     7381 cccttcgccg cgcgccagga actcgctgaa gtactgcagc gccggatgcg gccgcggcag
     7441 cgccatcaac tcgaggtagt cgtgctcgaa catgatgcaa tgattctgcg agccgagcga
     7501 atgcgtgccg cgcggcgtca ggctgaaccc catacgcgca taggcgcgct gtgccgcatc
     7561 cagatcccgc accaggatca ccgcatgatc gaggccttga acgaagcggc gcatcgccgt
     7621 cagggtgcct gtgcgatctc gagcgtcttc aggaagcgct cggcgtcctg gtagccgacg
     7681 acgcgcagcc cgcggatctc gcggccttgc gcgtcgaaaa agatgatgcc cggcgggccg
     7741 aaaagcgaaa agcgcttgag cagcgccttg tgctcgtcat tgttcgcggt gacatcggcc
     7801 tgcagcagca gcatgcgccc gaacgcctgg cgcacgcgcg ggtcgctgaa ggtgaacgcc
     7861 tccatttcct tgcacgacac gcaccagtcg gcatagaagt cgagcatcac cggcttgccg
     7921 ggcgcgcgca gcgccgcgtc gagctcggcg gacgagttca cgcgctgaaa cgcaatgggc
     7981 gcctcgggcg ctgcgcccgt gagccccgcc aggggtcgca gcggatcgcg gctgcccgag
     8041 agcgcgccga tcagcagggc gatgcccgcc accagcgcga atatgcccgc agccttggcg
     8101 atgcgcacgg gcccgctggc cggcgccggc aaccggtcca ccgcgtgcag atacacgccc
     8161 gtgcccacga gcagcgcggc ccaggccagc atcaccgcgg cgcttggcag taccggcgag
     8221 gcgatccaga tcgccaccgc cagcagcagc acgccgaaga atttctttac cccgttcatc
     8281 cacggcccag ccttcggcag cagcgcgccc tccgacacgc cgacgatcat caacggcacg
     8341 cccatgccga gcgccatggc aaagagcgcc gcgccgccga acgccacgtc gcgggtctgg
     8401 gagatgtaaa gcagcgcgcc ggcgagcggc gccgccacgc agggactgac gatcaccgcc
     8461 gagagcacgc ccatggccgc caccgaggca atgcgcccgc cgcgcaggcg ctggtgcgcc
     8521 gaggacaggc gatggtgcag gaatccgggc agcgcgatct cgtacagccc gaacatcgac
     8581 agcgccagcc acacgaacac ggcggcgaag gtgccgagca cccagggatt ctgcagcgcc
     8641 gcggcgatca acgtcccgga gtacgcggcg gcaatgccgg ccgccgcata cgcgagcgcc
     8701 atgccgagca catagctcgc cgacagcgtg aacgctcgcc gcttgttcag gttgcggcct
     8761 tcgccggcga tgattgatga caggatcggg accatgggca ggacgcacgg cgtgaacgtg
     8821 agcagcagcc cgaggccgaa gaacccggcg atgacggcca ggacgtgacg atcgaacagc
     8881 gcggcgatgt cccggtcgct ggcatagatc gaccagcgcg gcgtttcggg cacgcggcgc
     8941 ggtgcctcgc tgaacgccgg cgtcgatggc gccgcggcga ggagcagcgt ggcgcgcgat
     9001 tccatcggca cgtagcacac gccgacatcg gcgcagccct gcgaggtgac cacgagttga
     9061 agggggtcgg ccgtgcctgc ggccaccggc acgcggatgt gcacggcatt gcggtagacc
     9121 tcggtgtcgc cgaagaattc gtccttcttg cgcaggccgc caggcagctg cgccggcccg
     9181 agacgggcat tctgcgcctc gaacttgaag cgctcccggt acatgtagta gccgtcggcg
     9241 atggcgaagc tcacctcgac ggcgtcctcg cccaaggcgc gcgcggagaa gcgaaacgcc
     9301 ttctccggct cgagcagctc ttcggctgca gccgccagcg gcgccgccag cgccaaacac
     9361 agcaggagga atcggctcac ggggtcggtg tggtttccga ggccacccag tcgagatagg
     9421 cgggcaaacc ccgctcgact gggaccgcca cgatctccgg aagttcgtac gggtgcagcg
     9481 cccggatctc ggcctcgagc gcggcatagg cgtcgcgcgt ggtcttgatg agcaccgggt
     9541 gctcctcgtc gtgctgcacg gcgccccgcc accgatacac cgagcggcag ggcgcgagga
     9601 tgttgatgca cgccgcaacg cgcttcgcga cgagcgcgtc ggcaagcttc tccgccgcgg
     9661 cgcggtcggg caggttggtg aggaccagca aagcgctcac gcggcctgcc tggcgggcgg
     9721 cacaccctcg aatacggtgc ggtaacgcag ctgcacgcca agatcctcct tcatgcgccg
     9781 gttgaccagg cggcgcgact cgctcatgaa tgacagcagc tcgggcgaga ttcggcccgg
     9841 cgcatcgctg cgcgcgatgc gcggcggccg cggcagcccg gcgcgctccg ccacgaggtc
     9901 gagccagtcg cccatgcgga tctcgctgtc gtcgctcgcg ttgtaggtgc cgccgggagc
     9961 ggagtcctcg agcgcgcgca cggcgatcgc cgccaggtcc tcggcgtgga tatggttggt
    10021 gtacgcgtcg tcctcggcac gcagtacggg cgtgccgcgg cgcaggcgct ccagcggcag
    10081 ccggtcggcg gcgtagatgc cgggcgcgcg caggatcacg agcgtcgcgc cgcgcgcgct
    10141 gcaccatagt gcaagctgac gctcggcatc gacgcgccgc cgcgcgcggt cggtctgcgg
    10201 ctgcaccgcg cgcgcttcat cgacgcgcgc cccgccgcaa tcgccgtaca cgccgctcgt
    10261 gctgacgtag accacgcgcg ccggtagaat ccgggccttt gccagcgcgg cgagcaggtt
    10321 ccgggtgcgg gggtccgccg agcccgtggc gggcggcggc gcgcagtgca acacggcgtc
    10381 cgctgcaagg ccggacagcg tatcgggccg gtcgagatcg atgccgtgat ggcgcgacag
    10441 cgcctgcacc gcgtagcgcg cctcgagttg cggcgcggcg cggcgcgcaa tgtcgccgta
    10501 gcccgcgatt aagagtttct tcatcggtgc cattctagcg acatgcatag cgtcacgatc
    10561 cagccgagcg gtcagcactt ccaggtggag gacggcgaga ccgtgctggc ggcggcgctg
    10621 cgacaggggc tggtgctgcc ctacgggtgc aagaacggcg cctgcggcac ctgcaagggc
    10681 aggatcgtct cgggcacggt ggactacggc gcgtaccagg ccaaggcgct gcccgacgag
    10741 gacaaggcgc gcggcaaggc gctcttttgc caggcgaagc cgctctctga cctggtggtc
    10801 gaggcgcgca ccatcggcgc cgcgaaaggc atcgaggtca agatgctgcc gtgccgggtg
    10861 cagaagctcg agcggctcgc cgacgacgtc atggcactgc acctcaagct gcccgcaaac
    10921 gagaagctgg tgttcctcgc cgggcagttc atcgagttcc tgttgaagga cggcacgcgc
    10981 cgcagcttct cgatggccaa cgcgccgcac gacgcggagc tgctgcagct gcatgtacgc
    11041 cacgttgtag gcgggcagtt taccgaccac gtcttcggca agatgaagga gcgcgacatc
    11101 ctgcgtttcg agggaccgct cggcacgttc ttcctgcggg aggattccgc gaagccgatc
    11161 gtgttcgtcg cgtccggcac cggcttcgcg cccatcaagt cgattatcga ggcagcggtg
    11221 cacaagggcg tctcgcgacc catgaccctg tactggggcg ggcgccgccc caaggatctt
    11281 tacatgaacg cgctcgccga aggctgggcg gcgcagggcc tgctgaagta cgtgccggtg
    11341 atttcggagg cgctgccgga ggacggctgg tcggggcgta ccggcttcgt gcatcgggcg
    11401 gtgatggagg acctaccgga cctctccggc caccaggtct atgcctgcgg cgtgccggtc
    11461 atggtcgacg cagcgcgcac ggatttcacc ggatcgtgcg ggctgcccga ggacgagttc
    11521 tacgccgaca gctttaccac gcaggccgac gcgcccgcgc actagaaaaa gaaaaaggcc
    11581 cggggcttgc gccgccgggc cctcgcgcgc tgcgccgctg cgctacttat ttggcgtagt
    11641 cgtacttccc gtccttccag acgtagaaca cgtagcccgg cgcagtcaca tcgcccttct
    11701 tgtcgaagcc gatggtgcct agcacggtcg agaacttgcc cttgcgcagc gcgccggcga
    11761 cctctttcca cttcgtcgac ttcgcctggc cgacggccgc ggcccaggcc tggatcgcgc
    11821 cataggtgta gagcgtgtag ccctccggct catacttctg ggcgcggaac ttcttcacca
    11881 cgccctcggc ggccgggttc ttgcgcggat cggggctgaa ggtcatgagc gtgccctcgc
    11941 cggcggcgcc cgtgatcttc cagtactcgt cggtgaccag cgcgtcgccc gagacgagct
    12001 gcaccttgta gccctgctcg cgcgcctggc gcacgatgag gcccgcttcg gtgtggtagc
    12061 cgcccacgta caccacgtcg acgttctcgc ccttcatctt cgacaccagg gccgagtagt
    12121 ccttctcgcc cgcggtatag gcctcgtaca tggcttcctt cacgccgcgc ttgttcagct
    12181 gcttcttggt ctcgtcggcg aggcctttgc cgtacgcagt cttgtcgtga atgatgccga
    12241 tcttcttgcc cttgaacttg tcggcgaggt agttgccggc gaccgtgccc tgctggtcgt
    12301 cgcggccgca cacgcgaaac acgttcgggc cgccctcgtc ggtgagcttc gggttggtcg
    12361 aggcgggcga catctgcagg atgccttcct ccgtgtagac cttggaggcg gggatcgacg
    12421 agccggagca gaaatgcccg gcgacgaata ccaccttgtc gctcacgaac tggttggcta
    12481 cggcgaccgc ctgcttcgga tcgcaggcat cgtcgccgat cttcagcacc agcttcttgc
    12541 ccatcacgcc gcccttggcg ttgatatcgg ctaccgccat ctccgcgccg cggcgcatct
    12601 gctcgccgaa ggacgcgtac tgcccggtca tcgggccggc ggtggcgatc acgatctgcg
    12661 accaggcggc gctgccgaag ctcgccaggg cgagcgccag aatgaacttc aggataccgt
    12721 gcgttttcat cgggggaata cctcctcgga atgcagtgga attactctag cacagcgctc
    12781 agcctgcggg cggggtccgg ctgcgccagg aaaacggccc cgtacgctcg tacagccagg
    12841 ggtactggga caccatcttg cgcgcccggg ccaggcgcca ggcgaggagc gcaatgccgg
    12901 ccagggtcgc cgtatcgacg aggtaggccg caagcgacag cagcggcccg tcgaacagcg
    12961 cgaaggcgag aaaccggtcg gcaaggccga gcagcagcgt atagcccagg gcatgccagg
    13021 ccggcttcca ggtgatcgcg atcgcctgcc cggtcatgaa ggcggcgaag cccatcaggc
    13081 acaccgtcag gccgaagtaa acccccaggc cggtgccgag cagtgcctcc atcagcggcc
    13141 gccctccagg taggcccggc gcacttcggc gttggcgagc agctccccgc ccgtgcccga
    13201 aaggatgatg cggccgttcg ccatgacgta gccgcgatgc gcaatgcgca gcgcatggtg
    13261 cgcgttctgc tcgacgagga acacggtcac gccatcgcgc gtgttgattt cccggatcac
    13321 gctgaagatc tgcttgacaa gcagcggcgc gagcccgagc gagggctcat cgagcagcag
    13381 cagcttgggc cggctcatca gcgcgcgggc gatggcgagc atctgctgct cgccgcccga
    13441 gagcgtcccg ccgcgctgct cgcgccgctg gcgcaggatc gggtaaaggg tgaatacgcg
    13501 ctcgaggtcc tcgtcgaagt gccgcgggtc ggcgctcacc gcgcccatct gcaggttctc
    13561 gaccaccgac atgaacggaa acagccgccg gccctcgggc gcctgcgcca cgccgcggcg
    13621 cacgatctcg tgcgtcggca gccgcgtgat gtcttcgccg agaaaatcga tgcgtccctg
    13681 cgcggcgcgc ggattgccgc agatcgacat cagcagggtc gacttgcccg cgccgttggc
    13741 gccgatcagc gtgacgatct cgccttcggc gacatcgaca ttgatgccgt gcagggcctt
    13801 gatcggaccg taggcggcgt gcagatccgt gacgcggagc accgtcatgg ccgcgccgcc
    13861 tcctccgcgt cttccacgcc gaggtacgcg gcgatcacct tcgggtcgtt gcgcaccact
    13921 tcgggcgtgc cctcggcgat cttgcggccg tagtcgagca ccacgacgtg gtcggaaatc
    13981 tccatcacca cgctcatgtc gtgctcgatc aacagcagcg tcacgcggtg ctcgtcgcga
    14041 atgaacctga gcagctcgtt cagctgggcg gattcgcgcg ggttgagccc ggcggccggc
    14101 tcgtcgaggc acagcatcac cggctcggtg cacatcgcgc gcacgatttc gaggcgccgc
    14161 tgcgcgccgt agggcaggct gccggcgttg gcgtcggccc ggtcgacgag ctgcacgcgc
    14221 tccagccagt agcgcgcacg ctcgatggcg cgctgttcgg ccgccgcgta gccgccgatg
    14281 ccgaacaggg cgcccatcgt gtagcccgag gcgcgcatca gcgcgttgtg ctgggcgatg
    14341 agcaggtttt cgagcaccga catgccggtg aacaggcgga tgttctggaa cgtgcgcgcc
    14401 acgcccgccc gcgccgggat gtcgtagccc tccatgcgct cgagcaggaa ctcgccgcgc
    14461 cgcgggtggc gcaggcgcat gcagcccgcg gtcggcttgt agaaaccggt gaggcaattg
    14521 aacacggtcg tcttgccggc cccgttcggg ccgatgacgg cggtgatctt gcctgccggc
    14581 gcgtcgaacg agacatcgtc gaccgcgacc agcccgccga agcgcatcgt gaggtgctca
    14641 accgaaagca ggggcgtcat ttgggcggtc cgtggccaag ccgcacggtc ggctcgcggt
    14701 gcgcgagcag cccgcgcggg cgccaggtca tgatcgcgac catcgccagg ccaaaggcaa
    14761 gcatgcggta ctggttcaac tcgcggaaca cctcgggcaa accgatgagc aggatcgcgg
    14821 cgagcaccac cccgatctgg ctgcccatgc cgcccagcac cacgatggcg aggatgatcg
    14881 ccgactcgat gaacgtgaag ctctccgggc tgacgaagcc ctgacgggta gcgaagaagg
    14941 agccgccgaa gccgccgaac atggcgccga tggcaaaggc ggtcagcttg gtattgcgcg
    15001 ggtgcacgcc gaggctgcgg caggcgattt cgtcctcgcg cagcgcctcc caggcgcggc
    15061 cgatcggcag cttgcggatg cgcagcgtga acagattcgt caccagggcg agcgcgaccg
    15121 cgaggtaata caggaagatc acgcgatcga gcggcgtgta ctccagcccg aagaagctgt
    15181 ggaaggtggc gccgccgtca ggcgcctcgc gcgtcatcgg caggccgaaa aacgtcggcc
    15241 gcgggatgcc cgagaggccg tccgggccct tggtgaactg gtaccagttg agcaggatga
    15301 tgcggatgat ctcgccgaag ccgagcgtga cgatcgcgag gtagtcgcca cgcaggcgca
    15361 gtaccggaaa gccgagcagg atgccggcga aggccgccat gatgccggcg aacggcaggc
    15421 acacccagaa gccgagatcg aaattcagcg cgagcagcgc atagctgtag gcgcccacgg
    15481 catagaacgc gacgtagccc aggtcgagga gcccggccag gccgaccacg atgttgaggc
    15541 cccagcccag catgatgtag gtcgcgacca gggtggcgat gtcgagcatg cgccggtcga
    15601 tcccgggcag aaacggcagc gccaccgcca ggccgagcag cagcggaccc acccagccgg
    15661 cccacttctg cacctctgcg cccagccgat ccatgcgcgc ttcgcgcacc tccgcgctcg
    15721 tctcgctcgc cgcgtgccac accagccacg cggcacgcgc gctcagcagc caggcggcga
    15781 gcgcgatggt ggcattcaga accgtgctcc tgaagatcag cagctgctgt tcggcgccga
    15841 acagcaggat tccggtaagc gccagcgcga agaccagcgt gccggccagg accggcacgg
    15901 gacgctgcgc gcgcagcgcg ccgatggcga ggcgcccgaa gaaggcgatc accgccgccg
    15961 cgacgacatc gtcgaagcga taggccagcg gcggtccgcc ggaggtgtcc tggatgcgcg
    16021 cgccgaccag cgccagggtc agcaggacga caaccccggc gaccatcccg gcgtccttga
    16081 gcgcgccagg ccagtcgaac ggcgatcgcg gggcggcggc tgcgcgcatc agaccttctc
    16141 gacgtcgggc ttgcccagca accctgtcgg cctgaagatc agcaccagga tgaggatcgc
    16201 gaaggtcgcc acatccttgt actcgacgga gaagtacgcc gaccagaacg cctcgatcag
    16261 gccgatgagc agccccccga gcatcgcgcc ggggaccgag ccgatgccac cgagcaccgc
    16321 cgcggtgaac gccttgatgc cggcgagaaa gccgatgtag aagtcgatca cgccgtagtt
    16381 gagcaggaac atgagcccgg ccaccgaggc gaggctcgcg cccatgacga aagtaatcgc
    16441 gatggtgcga tcgacgttga tgccgaccag cgccgccata ccgagatcct gctggcaggc
    16501 gcgctgcgcg cgcccgagcg ccgtgcgcgt gatgatcagc gtgaagccga ccatcaggcc
    16561 cagggtgatc aggatgatca gcatctgcag aaagctgaac gccaccgcga attccccggt
    16621 ctcgaagaac ctcagaccgc cggtgacgac gggctgcagc ggcttcacgc gcgccccctg
    16681 cagcagttgc acgtagttct gcaggaagat cgacatgccg atcgcggtga tgagggcggc
    16741 gagccgcggc gagtggcgca acggccggta ggcgacgcgc tccagcgccc agccgtacac
    16801 cgaggtgagc agcatggtcg ccacgagcac gatgaccagc gcgagcggga tccagccgat
    16861 accgatcagg cccagggcaa ggaacgcgat gaaggcgatg aacgcgccga tcatgaagat
    16921 ctcgccgtgc gcgaaattga tcatgccgat gatgccgtac accatcgtat agccgatggc
    16981 gatcagcccg tagatcatgc caagcgtcag cccgttgatg agctgctgca ggaagtagcc
    17041 catggatcgt cccgtgccgc ggttgcgcgc gcgggaatgg ttacacaaaa aagggcgcgg
    17101 tgtcgcgccc agcgcggcct acgcgcggca ggcgttgcgc agcgcgtcga tgccgtcgaa
    17161 gggcggcagg catacggcac cgcggcacag ccaagcctgc acggcggccg accccggctt
    17221 gtcgagcagc ggcggcagac cgccgaggcc ggccgggatg gcgagcacca aagtccgtgg
    17281 caggtactcc cgtgccatcg cgccggtcca tgcgcgcagc gcttccggcg cgccgcgcac
    17341 aattgccgtg gcgggcggcg cgagccattc ttccagcgcc atcagcagcg ttgcgtagcc
    17401 cgcgggctgc gcgcgcacgc cgggaaggaa gagccgcaac gtgcgctcgg ccgcaacggc
    17461 ataacgggtt tcgccggtga gcgcggcaag ccggttcagt gcaagcgcgg ccgcgccgtt
    17521 gcccgaaggc gtggcctgat catggcccgg cttcggccgg tggatgaggc gctcgtggtc
    17581 gcgcgcggtg aagtagaacc cgcccgatgc gcggtcctcg aactgctcga agagcacgtc
    17641 ggccagtgct gtggcgaatg cgagatcctc ggcgcggaac tgcgcctgca gcagctcgac
    17701 gagcgccagc atcaggaacg catagtcgtc gaggtaggcg ttgagatgcg cgcgcccatc
    17761 cttgtaggtg gcgaggagcc tgccgccctg ccacatgcgc gagcgcacga aatcgagcgc
    17821 gcggcgcgcc gaggcgagcc acgcctgttc gccgaacacg cgtcccgcgc gcgccatgcc
    17881 gccaatggcg agcgcattcc aggacacgag cactttctcg tcgcgaccgg gccgcacgcg
    17941 cttgccgcgc gcggcgagga gcttcgcttt ggccgcggcg agcagagcct cgccgccggt
    18001 ccgcaatggg cgcgccacgc gcaggtgcca gtgacgccct tcgaagttgg gcgggccgtc
    18061 aagaccaaag tgcggcgccg ttaccgccca ctcctcggac gtgagcagcg cgcgcgcttg
    18121 gtccgcgtcc cacacgtagt acttgccctc ctcgtgctcg gaatcggcat cgagcgagga
    18181 ggtgtagccg ccttcgggcg actgcatctc gtgcatcagc cagcccgcgg tggccgcggc
    18241 gcagcgcgca tagagatcgt cgttcgtgac gcgccaggcg tcggcgagca gcgctagcag
    18301 cggcccgttg tcgtacagca tcttctcgaa gtgcgggatg cgccattcgg cgtccacgct
    18361 gtagcggcag aagccgccgc gcagctgatc gaagatgccg ccctcgcaca tgcgtcgcag
    18421 cgtcgtatcg acgatcgcgg tttcgccgcg gcgcaggcac agttcgagat cggtgggatg
    18481 cggaaacttg ggtgcgcccc caaagccgcc gttacgcgca tcgaagctgg cgcgcagcac
    18541 gtccagcgcc gcgcgcaccg gctcggcatc gagcgcttcg ccgccggcgg gcgtgcgcgg
    18601 ctgcagccgc tccaacgcga ggcgcacctg ctcgccctgc tcgccgaccg cggcacgctg
    18661 ctcgcgccag acctgcgcga tgcgctccag gatctcgacg aaccccggca gcccatagcg
    18721 cgcgcgcttc ggaaagtacg tgccgcccca gaacggcgtg ccatcgggcg cgagaaacat
    18781 ggtgagcggc cagccgccgc cgcgctgcgc cagcatctgg tgcgcggttt ggtagatctg
    18841 gtcgaggtcg ggccgctcct cgcggtcgac cttgatgttg acgaagtgcc ggttcatgac
    18901 cgcggctact tcggggtcct cgaagctctc atgcgccatc acgtggcacc agtggcaggc
    18961 cgagtagccg atggagagaa gaatcggctt gtcttcccgt tgcgcgcgcg ccagcgccgc
    19021 ggcgtcccac ggctgccagt cgacgggatt tgccgcgtgc tgctgcaggt agggcgaggt
    19081 ttcgccggca aggcgattgc tcatggctgg cgcaggatgt gctcgagtgt ctcgcggtag
    19141 accggcagcc gcgggccgcc gagcgcgacc gcgcggcgcg ccgccgccag cgcgtcgtcg
    19201 cggcttccca gttcggtcag cgcctgcgcg agattgttcc acgcgtcggc cgacccggga
    19261 tggcgctgcg ccgcgcgccg gtacgcggcc gcggcgccgg agaggtcgcg caacgcgtag
    19321 ctcgcgttac ccaggccgat ctgggcggcg acgctgtccg gccagcgcgc gagcgccgct
    19381 tcatagccgc ggcgcgccgc ggccgggtcg acgcgctcga gcgcggcaac ggcggcgagg
    19441 taggggcgct cggcgacggt ttccggcagc cttgccggcg gcagcgcgag catgccccaa
    19501 tgtccactgc gcgcccaagt gtgctcgaag gtgtcgaggc gcatggcaag gcggcgcgtg
    19561 acgccggagc gcagcacgag ctcgcgcgcc tcgaggtcgt agccgatcgc caccgcgtag
    19621 tgccactgcg gcgcccagtc aagcgccagg ttctgcagca ccagcaccgg cgtgcccgcg
    19681 gcaatctcgc gcagcagcgt gtcgagacgc ggctcgagcg tgtaggcgac gagcccatgg
    19741 cggcgcgtcg ccgcgagcat ctcggcctgg aaagacccct ggcgctccgg cacgtacact
    19801 tgcggcacca gcgatgccgg catcgcgttc cgccccgcgt gctgcagcac ggtggcgagc
    19861 gatgccgggc cgcactggta atcttcctgc ggaaagaacg gggtgtgcgt cagctcggcc
    19921 cgtacgggca ggttcgccgg agggcgttcg acgatgacgc tcgtctgcgg cgccgcgcag
    19981 ccggcaagca atactgccgc cgaaatgaaa acccccgccg cagcgggggt cgcgacctgc
    20041 cgcatgggcc gcttagcgca cggagcgcgt aaacggaaag atcttggtgt agcccatgac
    20101 gtcggtgaac agcagcacga ggaagatcag caccgcggcg ccgagcacgt cgccgcccgc
    20161 gggcagctcg tcgatctgcg cagcaaggtg cgccgcctcc tcgtcggtca gggcggcgac
    20221 ccgggcctgc gcctggaccg gatccacgcc gtacgccttc aggcgggctt gcacatcgct
    20281 gcgctcgagc aggcccgcca ggcgctcgtg ctgcgcgccg gcgaccagta cgtcgctgct
    20341 tacaatcccg gcatgcgcgg gcagtacaag tcccgtgccg cccaggcaga cgatgatgag
    20401 gccggcaacc atgcgacgaa aacgagtcag cattattcaa ttctcctttg ggggttgcga
    20461 atgcaaaaag ggtagtccgc gctcaccgtc agcgtcaacc tcggcttgtc ggcgcggcac
    20521 gacaggcgca agcacgccgt gagcgatccg cgcgcactcg aggtgctgcg gttctggttg
    20581 gacgagcatt ccaaggattg gttcgtgaag aaaccggacc tcgacgccga gatccggcgg
    20641 cgctttcacg cgctatacct cgaagccgca accggtcgcc tgcaggactg gctcgacgat
    20701 gcgcgcgagt gcctggccct cgtcatcctg ctcgaccagt ttgcgcgcaa catgtttcgg
    20761 ggcgaggcgc aggcgttcgc caccgacgcg cgcgcgcgcg cggcggcgcg cgtgatcctc
    20821 gatcacggct gggacaaggc gatgacgcag gccgagcagc tgttcgccta cctgccgttc
    20881 gagcacagcg agtcgctcga agaccaggac ctgtcgtgcg agctgatgaa ggatttcggc
    20941 gccgagcagc ggcgctacgc cgagcggcat cgtgaaatca tcgctcgctt cgggaggttc
    21001 cctcaccgca acggcattct cggacgcgaa agtaccgctg ccgagatcga atttctcaag
    21061 ctgccggggt caggtttctg aatcggtgat ggcgcctttc gaagccgtgg aaaccagttt
    21121 catgtacttc gccagcaagc ccctggtgta gcgcggcttg ggcgccttcc atcgcttacg
    21181 gcgcctggcc agctccttcg ccgggacatt cagccggacc aggtgtttct tcgcgtcgat
    21241 ggtgaccgag tcgccctcct ttaccagtgc gatggtgccg ccgacgtagg cctccggagc
    21301 cacatggccg accaccatgc cccaggtgcc tcccgagaaa cggccgtcgg tgagcaagcc
    21361 caccgaatcg cccaggcctt ggccaatcaa agcagaagta ggcgctaaca tttcctgcat
    21421 gcccgggccg cctttcggcc cttcgtagcg gatcacgacc acgtcgccgg ccttgatgcg
    21481 cttggccatg atcgccttca tgcaagcggg ctcggaatcg aacacgcgcg ccggcccggt
    21541 gatcgcggtc ttcttcagcc cggtgatctt cgcgacgcaa ccctcggtcg ccagattacc
    21601 tttgaggatg gcgagatggc cctggcgata aaggggattg gaccagggcc ggatcacgtc
    21661 ctggtcggca cgcggctcgg ccgggacgtc cttcagcatc tcggccatgg tcttgccgtg
    21721 gatcgtcatg cactcgccgt gcaggacgcc gttcttaagc aggatcttca gcacctgcgg
    21781 cacgccgccg gcgcgatgga agtccaccgc gacgtagcgc cccgacggtt tcaaatcgca
    21841 cagcaccggg actttgcgcc gcacgcgctc gaagtcgtcg atggtccact tcacgcccgc
    21901 ggccgaagcg atcgccaggt agtgcagcac ggcgttggtc gagcccccgg tcgccatgac
    21961 gagcgcgatc gcgttttcga tcgactttcg cgtgatgatg tcgcgcggct tgagattgcg
    22021 cttcaccgcc tcgaccagca cgcgcgccga ctcggcggcg ctatcggcct tctcgcggtc
    22081 gggcgaggcc atctgcgagg agccgagcag gctcatgccg agcgcctcga acgaggaact
    22141 catggtgttg gccgtgaaca tgccgccgca ggcgcccacc gaagggcagg cattcttctc
    22201 gatgccgacg aagtcctcct cgttcatgcg ccccgccgaa aatgcgccca ccgcctcgaa
    22261 cgccgagacg atggtgagat cctgcccctt ccacgtgccc ggcttgatgg tgccggcgta
    22321 gacgtagatc gccggcacgt tcatgcgcgc gatggccatc atgccgcccg gcatgttctt
    22381 gtcgcagccg ccgaccacca gcgcgccgtc catcgcctgg ccgttgaccg aggtctcgat
    22441 ggcatcggcg atgacttcgc gcgacacgag cgagtacttc atcgcctcgg tgcccatgcc
    22501 gatgccgtcc gtaatggtcg gcacgccgaa cacctgcggc ttggcgcccg cggcctcgag
    22561 cgccgccatc gcgctgtcca ccagcggctg gatgccggcg ttgcaggggt tcatggtcga
    22621 gtggccgttg gccacgccga cgatgggttt ctcgaaatct ccgtccttga accccaccgc
    22681 gcgtagcatc gcccggttgg gcgagcgggc gacgccctgg gtgatcagtt gtgagcggcg
    22741 gttgtcggcc atgcggcata tctccggaag tagaatgcaa attctaccgt gctgattcac
    22801 ccgcaattcg atcccgtcgc gctctccctc ggcccgctcg ccattcgctg gtacggcctg
    22861 atgtacctcg ccgcgttcgg cttcctgtgg tggctggggc gcatccgcat cgcgcgcggc
    22921 ctggcgccga tcggccgcga gcagttcgac gatctgatct ttctcggcgt gctcggcacc
    22981 attctcggcg ggcggctggg ctacgtgctg ttctacaagc ccgggtacta cctcgagcat
    23041 ccgctggaga tcttcgcgat ctggcagggc ggcatggcgt ttcacggggg cttcctgggc
    23101 gtgctggccg cggtggcgtt ctttgcttgg cgacgcggcg tggactggtg gcgcctgatg
    23161 gacttcatcg ctccgtgcgt gccgacgggc ctcgccgcgg ggcggctcgg caacttcatc
    23221 aatggcgagc tgtggggccg gccgaccgat ctgccatggg gcatggtgtt ccgcggcgcg
    23281 ggtagcctgc cgcggcatcc gtcgcagctc taccagtttg cgctcgaggg cgtgctgctg
    23341 ttcgtgctgc tgtggtggta tgcggcgaaa cccagggcgc gcggcgcggt ctcagccatg
    23401 ttcctcatgg gctacggcac cttccgcttc atcgccgagt tcgcccgcga gcccgacgaa
    23461 ttcctggggc tcctggcgct caacctgtcg atgggccagt ggctgagcct gccgatgatc
    23521 gcggccggcg ccgcgcttta cgtctggagc cgaggtcaga aatgaggtgg ctgctgctcg
    23581 ccctcgcgct cgcctgcacc gctctagcgg cacgtgcgca gtcacacgag gtgccctcct
    23641 ggtttgccga atcgtttctc gagttcccgc aggacgtgaa ggaagcggct ggcgatggca
    23701 agcgcctgat gctgtacttc tggcaggagg gctgccccgc ctgccggaag atgaaggaga
    23761 ccacgctcgc cgatccagcg ctcgtggagg cgacacgccg gcgtttcgtc gccgtcgcgc
    23821 tcaatgtgtt cggtgagcgc gagctgcagt ggaccgacgg gcgcacgctg cgcgaaaagg
    23881 agctcgcccg cgcgctcggc atccgcggca cgccgacgct gctgttcctg gacgagcgcg
    23941 gcgccgtcgt cttgcgccgg gtcggctacc tcccgcccga gggcttcgcc gcagcgctcg
    24001 cggaggcgaa gggccgctag ctctccagcg cgcgcgccag cacgcgcgcc acgtgctcgg
    24061 cgcggcgccc ggccccgtcg gcgatctggt ggcggcaact ggtgccgtcg gcgacgatga
    24121 gcgcctcggc cggcgcattg cgcaccgcgg gcagcagcga acgctcgccc atcttcatcg
    24181 acgcctcgta gtgctcggcc tcgtagccga agctgccggc catgccgcag cagctcgatt
    24241 cgatgatgga cacttgcagc gcgggcacgg tccgcagcac tcgctcgact gcacccatgg
    24301 cgccgaacgc cttttggtgg cagtgcccat gcacgagcgc ctgcgcggcg agcggcctca
    24361 gggccggcgc gaagcgcccg gcatcgagct ccgcggcgag gaactcctcg aagaggtagg
    24421 tgctgccggc ctgcgcgcgg gcctcgggca gcagcaccgg atactcgtcg cgcagcgtgt
    24481 acaggcacga gggctcgaga ccgatcacgg gcacgccgag cccaagccag ggacgcagcg
    24541 cttcgagcac gcgccgcgcc tcgacccgcg cctggtccag catcccggcg gtgaggaacg
    24601 tgcgcccgca gcacagcggt cgctcgccgg acgcaggctg cgccacgtgc acgcggtagt
    24661 ccgcggcctc cagcacgcgc acggccgcgc gcgcgttctc cggctcgaag tagcggctga
    24721 acgtgtccac cagcagcacc acttcacggc ggccgcgctt gggccagacg cggtcgacga
    24781 agtagtccga gcgccacggc ggcaattcgc gacgcggcgt gaacccgagc agggtgcgcg
    24841 tggcgcgcgc gcccagattg gccaggcccg ccgcgcgcgc cgcccacggt gcgtagcgcg
    24901 gcaggtaggc gatcaaccgc tcgcgcagcg tcgcgccgtg gcgcctgcgg tagtgcgcga
    24961 ggaactcgat cttcatgcgc gccatgtcga cgccggtcgg gcattcccgc ttgcagccct
    25021 tgcacgatac gcacaggtcg agggccgcct tcacgtcttc gtcggcgctc gcgccgagct
    25081 ggcccgagag cgcgaggcgc agcgtgttgg cccgaccacg cgtgaggtgc atctcgtcgc
    25141 cggtcgcgcg gaacgacggg cacatggtgc cggcgtcgaa cttgcggcag tggccgttgt
    25201 tgttgcacat ctccacggcc ttgtcgtaac cgccccattc gccccagtcg agcgcagtcg
    25261 acggcacctg ggtcgcgtag ccgggcttga agcggaacag cgccttgtcg tccatccggc
    25321 cgggcgcgac gatcttgccc ggattcatca ggccttgcgg gtcgagccag gatttgatct
    25381 cggccaggca cgccgtgagg cgcggcccga agaacggcgc gatccactcc gagcgcacca
    25441 gcccgtcacc gtgctcgccc gagtaggcgc ccttgtactg cttgacgagc gcgcaggctt
    25501 cttcggcaat ggcgcgcatc ttctcggcgc catcgcgccg catgtcgagc accgggcgta
    25561 cgtgcagtgt ccccaccgag gcgtgcgcgt accaagtgcc gcgcgtgccg tgcttctgga
    25621 atacctgcgt caggcgctcg gtgtactcgg cgagatgctc gagcggcacc gcgcagtcct
    25681 cgatgaagga caccggcttg ccgtcgcctt tcatcgacat catgatgttg aggcccgcct
    25741 tcctgacttc ccagacgtcc ttttgcagcc cggcatcggt gatctcgatg acgcttcccg
    25801 gcagtccgag atcgcccatc agctgcgaca gctgcttcag cttggaaacc tgagccggcg
    25861 cgtcgtcgcc ggagaactcg accagcagga tggcgtccgg ctcgccctgg atgacggcgt
    25921 cgaccgtcct gcggaaggcg ccgatgtcgc gcgcgaggcc gatcatcgtc cgatcgacca
    25981 gctccaccgc cgagggaccc agcttgacga tgtgctgcgt cagatccatc gcggtgtaga
    26041 acttcgggaa gtggcacacc cccagcgcgc gcgctggagg cagcggcgcc agccggagca
    26101 gcagccgttc ggagtaggcg agcgtgccct ccgagccgac caggagatgc gccgcgttcg
    26161 cgtgcggcgg tcccaggtga tcgaggttgt agccggcgac gcgccgcagc accttcggaa
    26221 agcgcgcagc gatctcgtcc ttctcgcgct cgtagagcgc gccgagcttt cgcaccagct
    26281 cgaggtagcc gggggggccc gaggcatccc gcatcggtcc gaagcgccag cgctcgcccc
    26341 cggccatgac ggcgtcgatg gcgagcacgt tgtgcaccat gttgccgtag gcgatggagc
    26401 gcgagccgca ggagttgttg cccgccatgc cgccgatcgt cgcctgcgcg ctggtggaca
    26461 cgtcgaccgg gtaccagagc ttgtgcgcgc gaagctgcgc gttcagctgg tcgagcacga
    26521 gccccggctg caccagcgcc gtgcgcccgg cggcgtcgac ctccagcacg cgattcaggt
    26581 actttgaatc gtcgatgacg agcgcggcgc cgaccgcctg gccgcactgc gacgagccgg
    26641 cgccacgcgg cagcaccggc acgcccgcct ccaccgcgat ggcgatcgcg gcgcgtgccg
    26701 cttcctcggt gcgcggcacg accacgccga cgggctcgac ctggtatatc gaggcatccg
    26761 tcgagtagcg gccgcggctc gccgcgtcga acagcacctc gccgtcgacc gccttgcgca
    26821 ggcgtgcagc gatcgaagcc tcgccggttc gcgggccgac gatgcgcacc gggcgtgcct
    26881 gagcgtccat ggctgcgggg tagaatacac tgcgccgcgc gacacgatgg gcgcgttcgc
    26941 tggggaggat gcatgccgcg caatgccgga cgacacttcc tgcagatccc ggggccgacc
    27001 aacgtgcccg accgggtgct gcgggcgatc gatttcccga cgatggatca ccgcgggccg
    27061 gagtttgcag acttgggcaa ggcggtgctc gagggcctgc ggcgcgtatt caagacgcgc
    27121 ggccacgtcg tgatctaccc cgcctcgggc acgggcgcgt gggaagccgc gctggtcaat
    27181 acgctctcgc ccggcgatgc ggtgctgatg tgcgaaaccg ggcacttcgc cacgctgtgg
    27241 cagaagatgg ccgtgaagct cggcctgaag cccgagttcc tgccgggcga ctggcgcagc
    27301 ggcgccgatc cccaggccat cgagaagcgc ctgcgcgcgg ataccgcgaa caacatcaag
    27361 gccgtctgcg tggtgcacaa cgaaacctcg actggcgtcg cttcgcgcat cgccgaggtg
    27421 cgcaaggcga tcgacgccgc cggacacccg gcgctgttca tggtcgacac catctcgtcg
    27481 ctcgcctcga tcgactaccg gcacgacgag tggggcgtgg acgtcacggt ggcgggctcg
    27541 cagaaaggcc tgatgctgcc gccgggcctt tcgttcaacg ccatctccga caaggcgctc
    27601 gccgccgcga aaagcgccgg gctgccgcgc agctactggg cctgggagga gatgctcgcc
    27661 agcggcaaga gcggctactt cccctacacg ccggcgacca acctccttta cggcctgcgc
    27721 gaggcgctcg ccatgctgga ggaggaagga ttggacaacg tcttcgcgcg ccaccagcgc
    27781 catgccgagg cgacgcggcg cgcggtgcgc gcctggggcc tggaggtgct cgccaccaat
    27841 ccggccgagt attcggcctc gctcaccgcg gtgctgctgc ccgccgggca cgacgccgac
    27901 cgcgtgcgca aggtcgtgct cgaggccttc gacatgtccc ttggcaccgg tctgggcaag
    27961 ctcgcgggca aggtgttccg cgtcggccac ctcggggatt tcaacgacct cgcgctgacg
    28021 ggcaccctcg cgggcgtcga aatgggtctt acgctcgcgg gcgtcccggt caagaatgaa
    28081 ggcgtagcgg ccgccatgcg ctatctcgcc gagtgccatc ggccgaaggc gcgcgcggcc
    28141 gcctgaaccc gccgcacatc caggaggaga aaaccatgaa gcttcgtgca tcccttgcag
    28201 ccctgttgct cgccgcgctc gcggcgcccg ccgccgcgca gaccgagctg cgcttcggcc
    28261 atgtcggcga gcccggctcg ctcatcgccg cttcggcgga ggagttcgcc cgcatcgcca
    28321 acgccaagct cgccggcaag gcaaaggtgg tggtgttcgg ctcgagccag ctcggcaacg
    28381 accgcgagct gctgcagaaa gtgaagctcg gcacggtgga tttcgcgctg cccaccacgg
    28441 tgatgtcctc ggaagtggac ctgttcggcc tgttcgagat gccctacctg gtcaaggacc
    28501 gggcgcacat ggcccgcatc gagaaggaga tcttctggcc gagcctcgcg ccgctcgccg
    28561 agcagaaagg cctcaaggtg ctcgcggtgt gggagaacgg ctaccgccac atcaccaaca
    28621 acaagcgccc gatcgtgaag ccggcggacc tgaacggcgt caagctgcgc acgccgcgcg
    28681 gcaagtggcg cgtgaagatg ttcgaggcct acggcgcgag cccgagcccg ctgccgttct
    28741 cggaagtgtt caccgcacta cagaccggag tgatggacgg ccaggaaaac ccgttcgcca
    28801 acatctggac cgccaagttc tacgaggtgc agaaatacct gtcgctgacc ggccacgtct
    28861 acaccccggg ttacccggtg gtcggcatca acaagtggaa gacgcattcc ccggagctgc
    28921 gcaagacgct cgaggacgcg gcaaaggaga cgcaggcgta cgtctacagc accgcgatct
    28981 ccatggagaa cgacttgctc ggcaagctca agtccggcgg catcaaggtg aacgaggccg
    29041 acaaggacgc gttcatcgcc ggctcgaaag caatttacga ggagttcggc aaggaagtgc
    29101 cgggcgcgca ggcgctcatc gacaaggcga tcgccctcgg caagtaagag cgacgccaag
    29161 gggccgctgt acgcggccct ttttttgcgt cccggctcgc atcgctcgtc gaccgccgca
    29221 ttgaccatgg ctgatcgcct gcgcacggcc ttcgagcgcc tgctcgaggt cgtggtcttc
    29281 gtgctgatga tcgcgctcgc gctgctcgtc gcggcggcgg tcgtgttccg caagttcaac
    29341 gcgccgatca tctggtacga cgaggtcgcg gtgatcctgc ttgcctggat cacctactac
    29401 ggctcggcgc tcgccgcgct caagggcgcc cacatttctg tctcggcagt agtggatgcc
    29461 ctgccgcgcg gcccgcgcat cgtcgtgacg ctgttcggcg aggcctgcgt catcgccttc
    29521 ttcctcgtgc tcggctgggc cggctaccag gtgctgggcg tgctcgccac cgactacctt
    29581 gtcacgctgc ccaaggtgtc gaacgactat gtgcagtcga tcatcccgat tggctcggcg
    29641 tgcttcgtaa tcgccgagct gctgcggctg cccgccgtgc tgcgcggcgc cgccgcgcgc
    29701 agcgaagcga gcccctgaca tggccgtcgt cctgctgttc ctcggggtgc tggcgctggt
    29761 gctgatcaac gtgccgatcg cggtggcgct cggcatcgcc gccgccgtcg ccatgatcgc
    29821 ggcgcaaggc gcgggcgcgc tgcccaacat ggcaatcgtc atgtactcgg gcaccaccgg
    29881 cttccccctg atcgcaatcc cgctgttcat cttcgccggc gcgatcatga acacttcggg
    29941 catctcgcgg cgtctgatcg cctttgcctc cgcgatcctc ggcttcatcc gcggcggcct
    30001 ggcgatggtg aacatcggca gctcgatgtt cttcgccgag atctcgggct cggcggtggc
    30061 cgatgtcgca gcgctcggct cggtgctgat gccggcgatg aagaagaaag gctatcccgg
    30121 taccttcgcc gcggcgatca cgtcgtcctc ggcctcgctc gccatcatca ttccgccctc
    30181 gattcccatg atcctgtacg cggtgatgtc gggcgcctcg gtggtgcaac tgttcgtggc
    30241 cggcatcatc cccggaatca tcggcgggct ggcgatgatg gcactcagct actggtacgc
    30301 ggtgcgcttc aactggccgg tcgaggaagt gttccagctc aagcgggtct ggaagacctt
    30361 caaggacgcc gcgcttgcgc tgctgctgcc ggtgatcatc cttggcggca tcttcggcgg
    30421 catcgtgacg gcctccgaag gggcgggcct ggcggtcgtg gcggccattg gcatcgccct
    30481 gtactaccgc gagatccagt ggcagcagct ctggacggcg gtaatcgacg gcgcagtgca
    30541 gaccgccgcg gtgatgctgc tggtggcggc aagcgcgctg ctcggcgtgt acctcaccga
    30601 agcgcaggtg ccgcagaagc tcgcgcaggt gatcgtcggc ctgaccaagg agccctgggc
    30661 ggtgctgttg ctgctcaatt tctttttcct gttcatcggc ctgttcctgc actcggccgc
    30721 ggccatcatc ctcgtcgttc cgatcgtcat gccggtggtg aacgcggtcg gcatcgatcc
    30781 ggtgcatttc ggactcatcg tcacgctcaa cctcgccatc ggccagcaga cgccgccggt
    30841 ggcgagcgtg ctgatgactg cgtgctccat cgccaaggag aacgtctggg acgtgaccaa
    30901 ggtgaatctg cccttcatcg gcgtgctcgt cagcgtgctg ctgctcatca cctacgtccc
    30961 cgccatcccg ctcgcgctgg tgaacctttt ctatcgctag ggccgccatg ccggagagcg
    31021 tctacgtcga gcgcgacggc gccatcgcca cggtggtgct caaccgcccg gacaagatga
    31081 acgccacgac ggtcgacatg tggctgcggc tcggcgatgc gtttcgtgcg ctgaatgccg
    31141 acgaggcgct gcgctgcatc gtgctgcgcg gcgccgggcg caaggccttc gccgccggcg
    31201 ccgacatcgg cgagttcggc aagacgcgca gcgacgcgaa agccgccaag gcctatagcc
    31261 ggcaggtcgg cggcaccatg cacgaaatcg cgcatgcccg ccacccgacg ctggccctga
    31321 tccacggcgc ctgcgtgggc ggtgggctgg agctcgccgc gctgtgcgac ctgcgcatcg
    31381 ccggcgagtc ggcgcgcttc ggcgtgccga tcaagaagct cggcctggtc gtcgcctacg
    31441 acgagatggc ggcgctcatc gcgctggtgg gccgtgctac aacgctcgag atcctgctcg
    31501 agggccgcgt gttcggcgcc gccgaagcgc tggccaaggg tctggtgacc cgcgtggtgg
    31561 ccgacgaccg gctggacgag gaagcgtacg ccgccgcgcg gcgcatcgcc gaaggcgcac
    31621 cgctcgcggc gcgctggcac aagaagttcg cgcggcgcct ggccgatccg cggccgctga
    31681 ctgaagagga gtacgacgaa agctatgcct gcttcgaaac cgaggacttc aaggaaggcg
    31741 ccgcggcgtt cctcgccaag cgcgcgcccc agttccgcgg ccgctaaggt cgggccgctc
    31801 gcgggcgtca aagtgctcga gctcgcgcag atcatggcgg gcccgacctg cggcatgctg
    31861 cttgccgacc tcggcgccga cgtcatcaag gtcgagcgca tcccgggcgg cgacgacacg
    31921 cgcagcatgg accggcactc gatcaacggc gaatcggcgg cgttcatggc catgaaccgc
    31981 aacaagcgcg gcatcgcgct gaatctcaag ctgccggccg cgcaggaagc gctgaagcgc
    32041 atggcggcca aggccgacgt gctcaccgag aactaccgca agggcacgct ggagaagctc
    32101 ggcgtgggct acgagacgct gaagaagatc aaccccggga tcatcttctg ctcgatttcc
    32161 ggctacggcc gcaccgggcc ctatgccgac aagggcggct acgacctcat cgcgcaaggc
    32221 atgtcgggcc tgatggcgat caccggcgag ccgggacgag cgccggttaa ggtcggcagc
    32281 cccgtctgcg acatcaacgg cggcatgctc gccgcgctcg gggtggtgtc cgcctacgtg
    32341 caccgcctga agaccggcaa ggggcagatg gtggacacct cgctcttcga ggcgggcatc
    32401 cagcaaacct actggcaggc ggccatgcac ttcgcgaccg gcgaatcgcc gggcccgggc
    32461 ggctcggcgc acccgctgtc ggcgccctac caggccttcc aggcgaagga cggctggctc
    32521 accattggcg gcgccaacca ggcgaactgg cagcgcatgg cgcgcgtgct cggcgcgccg
    32581 gagtggatcg aggatccgcg cttcctgaac aacacgcagc ggatgaacaa tcttcccgca
    32641 ctggtggcct tgatgaacga gaagctcgcg gcgaagacgg tgagcgagtg gctggcgctg
    32701 ctcgacgccg cgggcgtgcc ctgcggcccg atcaactcga tcgcgcaggt gttccaggat
    32761 ccgcacgcga tcgcgcgcga gatggtggtc gagctggagc acccgaaagc aggccacacg
    32821 cgcgccatcg gcctgccgat caagctctcc gcttcgccgg gcaaggtgcg gcggccggcg
    32881 ccgctcttcg gccagcacac gcgcgaggtc ctggccgagt tcggctttgg caagaaggag
    32941 atcgaggact tgatcggcag cggagcggcg atcgccgcct gagcatggac ttcacgctga
    33001 gcgaagagca gcggctgttt cgcgacagcg tgcgcggctt cgcggcaaag cacctcgccg
    33061 cgggcgcgct tgcgcgcgcg cacgacccgg cgcacccctt cgacgtcgcg cgcctgatgg
    33121 ccaagcaggg cctgctcggc atcacgatag ccgaggccga cggcgggcag ggtggcacgc
    33181 tgatggacgc ggtcctcgcc atcgaagcag tggcctcggt ctgcccgcgc agcgccgacg
    33241 tggtgcaggc aggcaacttc ggcccggtgc gcgtgctcgc cgagttcggc agccccgagc
    33301 agaagaagaa gtacctcgcg ccgatcctcg ccggcgaaac ggtgatgtgc ctcggcatga
    33361 gcgagcccga ggcggggtcg gcggtcaccg acctgaagac gagcgccacg ccggacggcg
    33421 acggctatcg cgtgaccggc aacaaggtgt tctcgaccca cgggccctac gcctctgtga
    33481 tcctcgccta cgtgcgctac ggcctggggg tcggcggcat cggctcggta ctgatcgagc
    33541 cgggctggaa gggcgtgacg ctcggcaagc cctcgcgctt catgtcgggc gaggaatggg
    33601 tcgagatcca cttcgaggac gtgcgcgtgc cgcgcgaaaa cgtgctcctc ggacccggcg
    33661 gattcaagaa acagatcgcg gggttcaacg tcgagcgcat cggcaatacg gcgcgctcgc
    33721 tcgccctggg ccgctacgcg tatgaggcag cccgtgagtg ggcgcttacc cgccaccagt
    33781 ttggccgggc gctggccgag ttccagggcc tgcagtggaa gttcgccgac atgaagatga
    33841 agctcgacgc cggggagctg ctcctttacc gcgcggcagc gaacgccgac cgcggcatgc
    33901 cctcggcaga ggagaccgct atcgccaagg cgttctgcaa ccaggccggc ttcgaagtgg
    33961 cgagcgaggc gctgcaggtg atgggcggca ccggctacag ccaggaactg ctggtcgagt
    34021 actgcctgcg ccgttgccgc ggctggatga tcgccggggg ctcgatcgag atgatgaaga
    34081 accgcatcgc cgaagggatc ttcgggcgca gcttcccgca gcggccgtga gcggcgaact
    34141 attccgtgcg ttgttcgcgc cgcgtggcat cgcgctgtac ggcgcgtccg gcgatccgtc
    34201 gaagaacacc gcgcggccgc agcgcttcct gcgcaagcat ggctatgcgg ggagcatcgt
    34261 tccgatcaac cgcacgcgcc gcgaggtcct cggcgagacg gcctacccga gcctcgccgc
    34321 cgcgccgggc gatatcgacc aggccttcgt gatggtgccg ccggcagacg tgccggccgc
    34381 ctgtgctgag tgcttcgcgc gccgcgtgcc cgtggtaacc atcttcaccg acggcttcgg
    34441 cgaaacggga gatgcggggc gccaggccga agcgcgcctg gtcgcggcgg cgaagtccgc
    34501 cggcacgcgc ctgctgggtc ccaacagcat cggcctggtc agcacctccc cgccggcgcc
    34561 gctgacggtc aacgcgacgc tcgagctcga gcgcctgccg gtcggcccgc ttgccgtggt
    34621 atcgcagtcc gggagcctga tcggcgcgct gctgtcgcgc ggcgcggcgc gcggcatcgg
    34681 cttctcgcgc ctggtgtcgg tcggcaacga agccgacctt tcggtcggcg agctgatgga
    34741 cctcctcgtc gacgatgcgc agaccggcgt gatcctgctg tttctcgaga cgctgcgcga
    34801 cgcgaagcgc atcgagcgtg cggcgcggcg cgcgttcgac gccggcaagc cggtgatcgc
    34861 ctacaagctt ggccgctcgg cggccggcgc ggcgctcgcg cgctcgcaca ccggcgcgct
    34921 cgcgggcgac gatcgggcag ccgacgcctt cttcggcgcc tgcggcatcc tgcgcgtacg
    34981 ctcgtttgaa gccctgatcg agacggcaca gctggcgatc ggacagcgcc cgcctcgcgg
    35041 caagcgcgtg gccgcgctga ccacgaccgg gggcggcgcg gcggtggttg ccgatccgct
    35101 tggcgctgcg ggcgcggagc tggtcgcgcc cccgcccgcg ctggccgaca cgatccgggc
    35161 gctcggggtg cgcgtcgcag actcgccgct tatcgacctc acgctcgccg gcacgcgcgc
    35221 caacgtctac ggcccggtgc tcgaagcgct cctcgcctcg gaccactgcg acgcggtgct
    35281 cgccgcggtg ggctcgtcgg cccaattcca tccacagctc gcgatcgagc cgatcctgga
    35341 agcgcaccgc aagcattccg gcaagccgct ggcggtgttc ctcgcgcccg aagcgcccgc
    35401 gtcgctgcag ctgctggcgc aggccggcat cgccgcattc cgcacgcccg agtcttgcgc
    35461 cgacgcgctc gcggcgtttc tctcctggcg cgcgccgcgg gcgccggcct cgcccgtctc
    35521 cttcgacata ccgcaggcgc ccacgcagcg gctgcgcacg ccggacgagc ccatcacgat
    35581 cccgttcccc gtggcgatca agatcgattc gcccgacatc gagcacaaga ccgaggtcgg
    35641 cggcgtcgag ctcgacgtgc gcgatgccaa agaggcggcg gcggcggccc ggcgcgtgct
    35701 cgagcgcgtg cgcggggcgt gcccccgggc ccgggtcaac ggcgtgacgg tctcgagcat
    35761 gcagaccggg cttgccgagg cgatcctcgg ctatcgcctc gacccgcagg tcgggccgct
    35821 cgtgttggtc ggcgccgggg gcacgctcgc cgagctctac cgcgacttcg cgctgcgcct
    35881 cgcgccggtg agcctcgagg aagcgcgcga gatggtggcc gaagtgcgcg gctttgcgcc
    35941 gctgcgcggc taccgcaacg cgccgcgcgg cgacctcgag gcgcttgccc gggcggtggc
    36001 cgcgcactcg cggctcgcgg cgcggcccga gatcgccgaa gccgagatca atcccctgat
    36061 cgtcaaggcc gacggcgtcg tcggcgttga cgcgctggtc gttctgcgcg aaggtgcgcc
    36121 cgatcatggc gctgccgctt gagaggctga tggagcgcgc cgcgggcgac gcgctgctcg
    36181 cgcgcttcgg gctggacttc gattgcgccc tgctggtcgg cgacggcgac accgactggc
    36241 tcgtcgagat cgcgcgcggc gcgatcgtgc gcgtggcgaa gggcccgttc atcatgccca
    36301 agaccgacct gcggctgcgc gcggcgagcg aggtgtgggg caaattcctc gcgcccgtgc
    36361 cgccgcccgg ctatcacgac ctcctcgcgc tgcgacgcta ccggcgcatc cagatcgaag
    36421 gcgatatccg cctcatgtcg gcgtacctgt tctacctgaa gcgcctgttc gcgctgctgc
    36481 gcgcacccgg cgcatgaaag cggcccgcga gccggtcacc ggctactacc tcaatgtcga
    36541 gatcctcggc cggccgcagc gcctgtatgt cgaggaggcg ggtcagggca ttcccctgct
    36601 gtgcctgcac acggcgggct ccgacacgcg gcagtaccgc gggctgatga acgatgccgg
    36661 catcctccgc gactaccgcg tcatagcgtt cgacatgccc tggcacggca agtcctcccc
    36721 gcccgcgggc tggcagaccg aggaatacag gctgacgagc gcagactacg cgcgcatggt
    36781 gctcgaggtg tgcgacgcgc tcgcgctgga gcggccggcc gtgatgggct gctcgatcgg
    36841 cgggcgcatc gcgctcgtgc tcgccatgga gcacgcgaag cgctttcgcg ccatcatcgg
    36901 cctgcaatcc ggcgcctcga tcgaccccta ttacgagctg acctggttcc atcatccgca
    36961 ggtgcatggc ggcgaggcag cggcggccta cgtcagcggg ctgatggcgc cgatgtcgcc
    37021 cgacacggac cgctgggaga cgctctggca ctacatgcag ggcggccccg gcatcttcaa
    37081 aggcgacctg tacttttatc gcggcgccga cggcgacatc cgcgagcgcc tgtccgcgat
    37141 cgacacccgc gtctgtccgc tgtacctgct cacgggcgag tacgactact cggtctcgcc
    37201 ggaggacacg cgcgccgcgg ccgagcgcat tccgggcgtg aagttcacgg tgatgccgaa
    37261 gctcggtcac tttccgatga gcgagaaccc ggccctgttc ctgtcgtacc tgaagccggt
    37321 gctggacgag atccgggcgc gcggctgacg ggcatgcgcg ccgtcgaacg ctgcggcgag
    37381 tttgtcgagt tcatgcgcga ggcgccgctc gccgcagagg tcctgcacca tgccaagcgc
    37441 gcggtcatcg actgggtcgc ggccgcgctg cccggtgcgg gggtgccgcc ggcgctcgca
    37501 ctcgaagcgg cgcttgtcga ggagctcgac cgcggcgcgg cgcaactggt gctcggccgt
    37561 ccggccaccg tgcgcgccgc agcgctcatc aacggcaccg ccgcgcacac ggcggaagtc
    37621 gacgacatct tccgcgacgg catctaccac cccggcgcac cgaccattgc ggcggcgctc
    37681 gcgctggcgc aggccggctc gctgagtggc ctgcgcttcc tccgcgcggt gatcgccggc
    37741 tacgaagtct ccacgcgcat cgcgcgcgcg atgggccgcg cgcattaccg cttctggcac
    37801 aacaccggca ccatcggcgc gttcggcgcc gccgcggcgg ccgcgggcgc gctgtcgctg
    37861 gaccgaaagc gcagcgccca tgcgctcgcc accgtggcca cgttcgcagc cgggctgcag
    37921 caggccttcc gcagcgattc catgtcgaag cctttgcacg cgggacgcgc ggccgaagcc
    37981 ggcgtcaccg cggcgctcgc cgcgcgcgag ggcgtgaccg gatcgctcga cgtcctcgac
    38041 ggcgcggccg gcttcggccg cgccatgggc gaaggccccg actgggtcga cgcgttcgcc
    38101 acgctcggca cgcactacaa cattgcgcgc atgacgttca agaaccacgc ctgctgcgga
    38161 catacgttcg cggccatcga cggcgcgctc gccgcgcgcg cggcgctcgg cgccgccgcg
    38221 ccggacatcg cgcgcgttcg catcggcagc tatcgcgccg cgaccgaagt ctccggcatc
    38281 gagaacccgc gcacggcggc cgaggcgcgc ttttccatca agtacgtggt ggcgcacgcg
    38341 cttcgccacg gcagcgtacg gcttgccgcg ttcgaggctg cgccgctcgc cgatcccgaa
    38401 acccgcgcgc tgatggggtg catcgaggcg gccgtcgatc ccgaactcga cgccgcgttc
    38461 cccgggcagc gtgccgcacg cgtggcgatc gaaacacgcg acgggcgcag ccacgctctg
    38521 ctgcagccgg cgcgcaaggg cgatcccgag ctgccgctcg acgacgccga gctcgaggcg
    38581 aagtacttgg agctggcggc gccggtgatc ggcgccgcgc cggcgcgtgc gctactcgcg
    38641 cgcctgtggc ggctcgagcg cgagcccggc gtcagcctcg cggccgcagc accgccacga
    38701 gcggcaaggt gagcaggatc gtcccgccca gcgccgcgat ggcggcctgc accccaagct
    38761 ggtcgccgag cagcccgaag gtcagcggcg cgacgatgcc gcagaccgaa cccaccgtat
    38821 agaacagacc gaaagcgcgc gacagccgct ccggcctgac gtactcgccg atcgtggcgt
    38881 acaggaccga cgacgtcccc tggacgacga cgccaagcaa cggcaacagt agcagcgccc
    38941 ccgcggcggg cagggcaagc acggcgaaga tgccggccgc ggtcgcgatc tcggtggcga
    39001 cgatcatgcg cggcacgccg atgcgctcgg cgagcacgcc gcaggcgagc ttgccggcca
    39061 tgccgccggc gagcaccagg ggcaccgcgg ccaccgccca gcccgcggac agccctttgg
    39121 cgagcagcag gaaggcgatg aaggtgagaa aggcgctgcg cgtgctcgcg tcgagaacct
    39181 cgatcgcgca caacgcgaga aagcgcgggc ggtcccggat gccccagtcc atagcgcgat
    39241 cccccctcga cgcatgctgc ggcgccggcg cccttcccgc agccaacaac gcaagcaccg
    39301 ccaccgccac tgcaatggcg gcgacgccga atccgatcac cggcgcctgc cacgcgaccc
    39361 ccgccagcag cagcagcgag accagtcccc caaacgcgaa cttgcccacg tcgcctgcga
    39421 agttgtacgt gccgagcgcg gcgcgccgtc cctggaccgg ataggcacgc gacaccagcg
    39481 tcgaggcgag cggatgctgc accgcagagc ccatgcccgc gaagaacagt gccgcaagga
    39541 tcgcccagaa gccttgcgcg tagccgagcg cgatgaacgc cgcgcctgcc agcgcagtgc
    39601 cgaacgcaag caggccgcgc tcgccgaccc tctcggccag gatgccagcc ggtatctgga
    39661 acgccgccat ggccgcgcgg tgcgcgccgc gaatgatgcc gacttgcgcg agcgacagcc
    39721 cgaacgcatt cgcgagcagc ggcagcagga cgtaggtaac gtcggatagc ccgtcgtgca
    39781 gcgagtgcac cgcgctgcaa gtcgtcagcg tgcgtttgct catcggcgat ccggcaggag
    39841 ctggattttc ggcatggtag actgcccagc ccgtggccac cctactagga gggggaaggt
    39901 ggcgaagctg ctcgaagtca agaacctgca gacgcaattt ctcacgtccg ccggcgtcgt
    39961 gcgcgccgtc gacggcgttt cctacgacat caacgagggc gagaccgtcg cgctggtcgg
    40021 cgaatcgggc tgcggcaagt cggtgtcggc gctgtccatc atgcgtctgg tcgccgagcc
    40081 cgcggggcgc atcgtcggcg gcgagatcct gttcaaggga cgcgacctgc tcaagttgac
    40141 cgaggaggag atgcgcgacg tgcgcgggcg cgacatcacg atgatcttcc aagagccgat
    40201 gacctcgctc aacccggtgc tttcgatcgg gcgccagctc acggagggtc tcgagatcca
    40261 cctcggcatg aaccagaagg cggcgcaggc gcgcgccgtg gaactgctcg gcctcgtcgg
    40321 cattccggac ccgcagcgcc gcctcacgca gtatccgcac cagttcagcg gcggcatgcg
    40381 ccagcgcatg atggtcgcca tggcgctcgc ctgcaatccg aagctcatcc tcgccgacga
    40441 gcccaccacg gcgctcgacg tcaccatcca ggcgcagatc ctggagctgt tgcaggacct
    40501 ttcgcgccgc ctgggcgtcg cgatgctgat catcacgcac aacctgggcg tggtggcgcg
    40561 ctacgccgat cgcgtcaacg tcatgtacgc cggacgcgtc atcgagcaag gcagcgctgc
    40621 ggagatctat gccgatccac gtcaccccta cacactcggg ctgctgcgct cggtgccgcg
    40681 cctcgatgag ccgatgcgcg ccaagctcga tccgatcgaa gggcagccgc ctgatctgac
    40741 caggctgccg cccggctgcg cgttcgcgcc gcgctgcgcc ttccgcgtgg accgctgcac
    40801 cgagacgccg ccgctcacgg cgctcggcgc gcccggacac ctgtcggcct gctgggaggc
    40861 cgagcacttg gcgaaggagg tgccggcatg agtcagccgt tgctcgaagt gcatggcctc
    40921 gtgatgcact tcccggtggg cggggggatg ttctccaagc cggccggcgt ggtgcgcgcg
    40981 atcgacggag tgagcttcaa catccgcaag ggcgagacgc tcggcctcgt cggcgagtca
    41041 ggctgcggca agaccactac cgggcgctgc atcctgcagc tcgagcgtcc cacgggcggg
    41101 cgcatcgtct tcgagggcgt cgacatgatg gcgctcgacg ccgccgagct gcgcgccgtg
    41161 cgccgccgcg tccaggtaat ctttcaggac ccgtattcct cactcaaacc gcgca
//
LOCUS       DBNHKE010000009         3764 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_583293, whole genome shotgun sequence.
ACCESSION   DBNHKE010000009 DBNHKE010000000
VERSION     DBNHKE010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_583293"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC16O9_00565"
     CDS             complement(<1..352)
                     /locus_tag="AC16O9_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR6515144.1"
                     /translation="MTGYALASAEHPRGALSLELRSVNARFLDLQFRVPEELRALEPV
                     LREAIVARLTRGKVDCRLAFAAGAGSAPALDAAALESLRQLEAAARRAFPDAAPLAAS
                     DVLRWPGVVRDGTLD"
     gene            418..1365
                     /locus_tag="AC16O9_00570"
     CDS             418..1365
                     /locus_tag="AC16O9_00570"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6515145.1"
                     /translation="MPAQANLPLPEGHQLLNYRILRLLSHGGFSFVYLAHDENEAPVA
                     IKEYLPATLALRTAGGPLPVVPEAHAAAFRYGLKCFFEEGRALATLAHPNVVRVLNFF
                     RANETVYIVMRYERGRTLQEHIHARGGVLREAWIRDTFASLLNGLREVHSNKLLHLDI
                     KPGNVYLRGDGTPLLIDFGAARTMLAGEAPVLPPMYTPGFASPEHHADRSKLGPWSDI
                     YSVGATLYACLVAEAPPPAEERMEKDRYVPARRAGGGKFTPELLDTVDWCLRLNHLER
                     PQSVFALQKALTGEKPPEYRGATPVLERIKGALARFSRR"
     gene            1362..2267
                     /locus_tag="AC16O9_00575"
     CDS             1362..2267
                     /locus_tag="AC16O9_00575"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6515146.1"
                     /translation="MKCTIYQETHVGKRPYNQDRLGHWRTPEALLLVLADGMGGHAHG
                     EVAAELTMRHIAAAFKHEAQPRLANPDLFLFRAVGRSHGMLLREARALGLTESPRTTV
                     VACVVQEGRAYWSHVGDSRLYLVRKGEVLVRTRDHTLVQQLIDAGRLREEDAGSHPER
                     NRLLQCVGGAHAPRISPTAHARLERDDIVLLCSDGLWGPLMERQIIAGLESGPLPEAI
                     ARLASLAQERAGPSSDNVSALGLAWHEDAVPLRDGPRTVPLAEMSTEVQDFGATNPEF
                     LRMTDGDIERAVAEIKDALKKRAPR"
     gene            2264..2851
                     /gene="rdgB"
                     /locus_tag="AC16O9_00580"
     CDS             2264..2851
                     /gene="rdgB"
                     /locus_tag="AC16O9_00580"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR6515147.1"
                     /translation="MKRLVLASNNAGKLREMRALLAPLGCEVIAQAELGIAETEEPHD
                     SFLENALAKARHASRASGLPALADDSGLCVDALGGAPGVHSAYFAGREGSRAERDARN
                     NAKLLASLGQARTAHYRCVMALVRSADDRQPLIAEGVWHGGIARAPRGANGFGYDPLF
                     LIDGDGRTAAELPEAEKNRISHRGRAAARLLELLR"
     gene            2848..>3764
                     /gene="hemW"
                     /locus_tag="AC16O9_00585"
     CDS             2848..>3764
                     /gene="hemW"
                     /locus_tag="AC16O9_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6515148.1"
                     /translation="MSAVVRAPHEVRFAALPPLALYAHLPWCVRKCPYCDFNSHEKAD
                     ALPEREYVEALVADLEGMLPLVWGRRVTSVFIGGGTPSLFAPESIDALLAAIRARVPL
                     EPGAEITLEANPGTAEAGRFRGYREAGVNRLSLGIQSFDDEKLKVLGRIHSAAEARRA
                     IEMAQAVFDNVNLDIMYGLPQQTLKETRTDARAALAFGTTHFSAYQLTLEPNTVFYSK
                     PPPLPVHDTCADMQLAVEEIAAEAGFEHYETSAFARPGRRCRHNLNYWEFGDYLGIGA
                     GAHGKLSFADHVTRHERAKQPREYMAQAG"
BASE COUNT          577 a         1351 c         1346 g          490 t
ORIGIN      
        1 cgtcgagcgt gccatcgcgg accacgccgg gccagcgcag cacgtcgctg gccgcaagcg
       61 gcgccgcgtc cggaaacgcg cggcgcgccg cggcctcgag ctggcgcagc gactcgagcg
      121 ccgcggcatc gagcgccggc gcgctgcccg cgcctgccgc gaaagcgagg cggcagtcca
      181 ccttgccgcg cgtgaggcgc gcgacgatgg cctcgcgcag cacgggctcg agcgcgcgca
      241 gctcctcagg cacgcggaac tgcaggtcga gaaagcgcgc gttcaccgag cgcagctcga
      301 gcgagagcgc gccgcgcgga tgctcggccg aggcgagcgc gtaaccggtc atgctgcgga
      361 tcaaggaggc agttcgcgga gcaaagcgtg cgaatcgaag cgggtatcat aggggagatg
      421 cccgcccaag ccaacctgcc gctgccggag gggcatcagc tgctgaacta ccgcatcctg
      481 cgcctgctgt cgcacggcgg cttttccttc gtgtacctcg cgcacgacga gaacgaggcg
      541 ccggtggcga tcaaggagta cctgccggcg acgctcgcgc tgcgcaccgc cggcgggccg
      601 ctgccggtgg tgccggaggc gcatgcggcg gcatttcgct acgggctgaa gtgcttcttc
      661 gaggagggcc gcgcgctcgc cacgcttgcc cacccgaacg tggtgcgggt gctgaacttc
      721 ttccgcgcca acgagaccgt atatatcgtg atgcgctacg agcgcggccg cacgctgcag
      781 gagcacatcc acgcgcgcgg cggcgtgctc agggaagcgt ggatccgcga caccttcgcc
      841 agcctgttga acgggctgcg cgaggtgcac tccaacaagc tgctgcacct cgacatcaag
      901 cccggcaacg tgtacctgcg cggcgacggc acgccgctgc tcatcgattt cggcgccgcg
      961 cgcaccatgc tcgccggcga agcgccggtg ctgccgccga tgtatacgcc gggctttgct
     1021 tcgccggagc accatgccga ccgctccaag ctcggcccct ggagcgacat ctactcggtg
     1081 ggcgcgacgc tttacgcctg cctggtggcg gaggcgccgc cgccggccga ggagcgcatg
     1141 gagaaggatc gctacgttcc cgcgcggcgc gccggcggcg gcaagttcac gcccgagctg
     1201 ctcgacacgg tggactggtg cctgcgcctg aaccacctcg agcggccgca aagcgtgttc
     1261 gcgctgcaga aggcactcac cggcgagaag ccgccagagt accgcggcgc tacgccggtg
     1321 ctggagagga tcaagggggc gctcgcgcgc ttttcgcgcc gatgaagtgc acgatctacc
     1381 aggaaaccca tgtcggcaag cggccctaca accaggaccg gctcgggcac tggcgcacgc
     1441 ccgaggcgct gctcctggtg ctggccgacg gcatgggcgg gcacgcgcat ggcgaggtgg
     1501 cggccgagct caccatgcgc cacatcgccg ccgccttcaa gcatgaggcg cagccccggc
     1561 tcgcaaatcc cgacctgttc ctgttccgcg ccgtgggccg ctcgcacggc atgctgctgc
     1621 gcgaggcgcg cgcgctcggg ctgacggagt cgccgcgcac cacggtcgtt gcctgcgtgg
     1681 tgcaggaggg ccgtgcgtac tggtcgcacg tcggcgactc tcgcctgtac ctggtgcgca
     1741 agggcgaagt gctggtgcgc acgcgcgacc acaccctggt gcagcagctg atcgacgcag
     1801 gccgtctgcg cgaggaggac gccggatcgc accccgagcg caatcggctg ctgcaatgcg
     1861 tgggcggcgc gcacgcgccg cgcatctcgc ccacggcgca tgcacgcctg gagcgcgacg
     1921 acatcgtgct gctgtgctcg gacggcctgt ggggcccgct tatggagcgc cagatcatcg
     1981 ccggcctgga aagcggcccg ctgcccgagg cgatcgcgcg cctcgcgtcg ctcgcccagg
     2041 agcgcgccgg ccccagcagc gacaacgtgt cggcgctcgg gctcgcctgg cacgaggacg
     2101 cggtgccgct gcgcgacggc ccgcgcaccg tgccgctcgc cgagatgtcc accgaggtgc
     2161 aggatttcgg cgccaccaac ccggagttcc tgcgcatgac ggacggcgac atcgagcgcg
     2221 ccgtcgccga gatcaaggac gcgctgaaga agcgcgcgcc gcggtgaagc gcctggtgct
     2281 cgcgtcgaac aacgccggca agctgcgcga gatgcgcgcg ctgctggcgc cgctcggctg
     2341 cgaggtcatc gcgcaagccg agctcggcat cgccgagacg gaggagccgc acgacagctt
     2401 cctcgaaaac gccctcgcca aggcgcggca cgccagccgc gcgagcgggt tgccggcgct
     2461 cgccgacgat tcgggcctgt gcgtggacgc gctcggcggc gcaccgggcg tgcactcggc
     2521 ctacttcgcg gggcgcgagg gcagccgcgc ggagcgcgac gcgaggaaca acgccaagct
     2581 cctcgcgagc ctcggccagg cgcgcaccgc gcactaccgc tgcgtgatgg cgctggtgcg
     2641 aagcgctgac gatcggcagc cgctcatcgc cgaaggcgta tggcatggcg gcatcgcgcg
     2701 cgccccgcgc ggtgcgaacg gctttggcta cgacccgctg ttcctcatcg acggcgacgg
     2761 ccgcaccgcg gccgagctgc cggaagcgga gaagaaccgc atcagccacc gcggccgcgc
     2821 cgcggcgcgg ctgctggagc tcctgcggtg agcgccgtgg tgcgcgcgcc gcacgaggtg
     2881 cgcttcgcgg cgctgccgcc gcttgcgctc tacgcgcacc tgccctggtg cgtgcgcaag
     2941 tgcccgtact gcgacttcaa ctcgcacgag aaggcggacg ccttgcccga gcgcgagtac
     3001 gtcgaggcgc tcgtcgccga cctggaaggc atgctgcccc tggtgtgggg ccggcgcgtg
     3061 acgagcgtgt tcatcggcgg cggcacgccg agcctgttcg cgcccgagtc gatcgatgcg
     3121 ctcctcgccg cgatccgcgc gcgcgtgccg ctcgagcccg gcgccgaaat tacgctggag
     3181 gcgaacccgg gcaccgcgga ggcggggcgc tttcgcggct accgcgaggc cggcgtgaac
     3241 cggctgtcgc tcggcatcca gagcttcgac gatgaaaagc tgaaagtcct cgggcgcatc
     3301 cactcggcgg ccgaggcgcg ccgcgccatc gagatggcgc aggcagtgtt cgacaacgtg
     3361 aacctcgaca tcatgtacgg gctgccgcag cagacgctaa aggagacgcg caccgacgcg
     3421 cgtgcggcgc tcgccttcgg cacgacgcat ttctccgcct accagctcac gctcgagccg
     3481 aacaccgtgt tctacagcaa gccaccgccg ctccccgtgc acgacacctg cgccgacatg
     3541 cagctcgcgg tggaggagat cgcggccgag gccggattcg agcactacga gacctcggcc
     3601 ttcgcgcgcc cgggacgacg ctgccggcac aacctcaact actgggagtt cggggactac
     3661 ctcggcatcg gcgccggcgc gcacggcaag ctctcgttcg ccgaccacgt cacgcgccac
     3721 gagcgcgcga agcagccgcg cgagtacatg gcgcaggcgg gaag
//
LOCUS       DBNHKE010000010         2789 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_795045, whole genome shotgun sequence.
ACCESSION   DBNHKE010000010 DBNHKE010000000
VERSION     DBNHKE010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_795045"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1118
                     /locus_tag="AC16O9_00590"
     CDS             111..1118
                     /locus_tag="AC16O9_00590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6515149.1"
                     /translation="MSIKQNFTSDALPDFRNLGVVARILLGVNALAFAAVLAAAPSWG
                     AALERFLLVAARLEPALAVTLVVLYALSPALARLPYWQGGFAVVTLTLAVSGTLHVAS
                     AAAGDALAGLARTLALAAIAALLLLAYLRLLTRAYSPALAEARLQALQSRIRPHFLFN
                     SLNAVLSLIRRDPRRAEQALEDLSDLFRVLMQDNRNFVPLGDEIALLERYASLEQLRL
                     GERLKVVWELEGAPRDALMPPMILQPLLENAIYHGVEPGAGEGRVVVHIERRGTRVFA
                     RIENPYIEQHAHRAGNRMALENIRERLQLFFDIEARVDMRVAGERYVVEIAMPYRPGK
                     D"
     gene            1123..1875
                     /locus_tag="AC16O9_00595"
     CDS             1123..1875
                     /locus_tag="AC16O9_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR6515150.1"
                     /translation="MLRVFIADDETPARERLKELLADIAAELPTEVVGEAASGLDAVA
                     QLPASRAQVLLLDIEMPGMRGLEVARHLSGLELAPAVVFVTAHDRHAVEAFELNALDY
                     LLKPVRAARLAAALKKASADGPPERERLAKAAGQAREYLSVTERNRIHLVPVRDIVYL
                     RAELKYVTLRTRAGEHLIEESLVALEREFAERLVRVHRNCLVARAAVRGFERSAEGGE
                     EPRWNVVLEGVPERLPVSRRQWAEVRVLAKEG"
     gene            1876..>2789
                     /gene="hemC"
                     /locus_tag="AC16O9_00600"
     CDS             1876..>2789
                     /gene="hemC"
                     /locus_tag="AC16O9_00600"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR6515151.1"
                     /translation="MRARLTIATRRSRLALWQAEHVKARLEAEHRGLEVALLPLSTRG
                     DELLEVRLDKVGGKGLFVKELEQALADGRADLAVHSMKDVPVALPAGFVLAATLERED
                     ARDALVSNAYAELAALPPGARVGTSSLRRAAQVAARYPRLELATLRGNVETRLAKLDR
                     GEYDAVLLAAAGLKRLGLEARIRCCLPVDESLPAPGQAALGIECLASRADVAALLAPL
                     AHAASAACVRAERAVSRSLAGSCHVPLAAFAVIEGETLWLRGLVASPDGKQVARGELR
                     VPVAEPERAGSELAALLRSRGADKILAGL"
BASE COUNT          352 a          971 c         1083 g          383 t
ORIGIN      
        1 ggcgaccagg gcgccgcggg acgccccgcc ccggctctag ggcgcgactt ccgcttggtg
       61 cgaggagggg ccatggggca tacttggtcg cggcgcgccg cgctatgcca atgagtataa
      121 agcaaaactt cacgagcgac gccctgccgg acttccgcaa cctgggcgtg gtggcgcgca
      181 tcctgcttgg cgtcaacgcg cttgccttcg cggcggtgct cgcggcggcg ccgagctggg
      241 gcgcggcgct cgagcgtttc ctgctggtgg cggcgcgcct cgagccggcg cttgccgtga
      301 cgctggtcgt gctctacgcg ctgtcgcccg cgctcgcgcg gctgccgtac tggcagggcg
      361 gcttcgcggt ggtgacgctc acgctcgcgg tcagcggcac gctgcacgtg gcgagcgcgg
      421 cggcgggcga cgcgctggcg ggccttgcgc gcacgctcgc gctcgcggcg atcgcggcgc
      481 tgctgctgct cgcgtacctg cgcctgctca cgcgcgccta ttcgccggcg ctggcggagg
      541 cgcggctgca ggcgctgcag tcgcgcatcc gcccgcattt cctgttcaat agcctgaacg
      601 cggtgctgtc gctgatccgc cgggacccgc gccgcgcgga gcaggcgctg gaggacctgt
      661 cggacctgtt ccgcgtgctg atgcaggaca accgcaactt cgtgccgctc ggcgacgaga
      721 tcgcgctgct cgagcgctac gcgagcctcg agcagctgcg cctgggcgag cggctgaagg
      781 tggtgtggga gctggaaggc gcgccgcgcg acgcgctgat gccgccgatg atcctgcagc
      841 cgctgctcga gaacgcgatc taccacggcg tcgagccggg cgcgggcgag gggcgtgtcg
      901 tggtgcacat cgagcggcgc ggcacgcgcg tcttcgcgcg catcgagaat ccgtacatcg
      961 agcagcatgc gcaccgtgcc ggcaaccgca tggcgctcga gaacatccgc gagcggctgc
     1021 agctattctt cgacatcgag gcgcgcgtcg acatgcgcgt cgccggcgag cgctacgtgg
     1081 tggagatcgc gatgccgtac cgcccgggaa aggactgagg caatgctgcg cgtgttcatc
     1141 gctgacgacg agaccccggc gcgcgagcgc ctgaaggagc tgctcgccga catcgccgcc
     1201 gagctgccca ccgaggtggt gggcgaggcg gcgagcgggc tcgacgcggt ggcgcagctg
     1261 ccggcgagcc gcgcccaggt gctgctgctc gacatcgaga tgcccggcat gcgcgggctg
     1321 gaggtggcgc gccatctctc cggcctggag ctcgcgcccg cggtggtgtt cgtcacggcg
     1381 cacgaccgcc acgcggtgga ggccttcgag ctgaacgcgc tggactacct gctcaagccg
     1441 gtgcgcgccg cgcgcctcgc ggcggcgctg aaaaaggcga gcgcggacgg gcccccggag
     1501 cgcgagcggc tggcgaaggc ggcggggcag gcgcgcgaat acctgtcggt gaccgagcgc
     1561 aaccgcatcc acctcgtgcc ggtgcgcgac atcgtctacc tgcgcgccga gctcaagtac
     1621 gtgaccctgc gcacgcgcgc gggcgagcac ctgatcgagg agtcgctggt ggcgctggag
     1681 cgcgagttcg ccgagcggct ggtgcgcgtg caccgcaact gcctggtggc gcgcgccgcg
     1741 gtgcgcggct tcgagcgctc ggccgagggc ggcgaggagc cgcgctggaa cgtggtgctc
     1801 gagggcgtgc ccgagcgcct gccggtcagc cgccggcagt gggccgaggt gcgcgtgctg
     1861 gcgaaggagg gctgaatgcg cgcgcgactc acgatcgcga cgcgccgcag ccggctggcg
     1921 ctgtggcagg ccgagcacgt caaggcgcgg ctcgaggccg agcaccgcgg gctggaagtg
     1981 gccttgctgc cgctctcgac gcgcggcgac gagctgctcg aagtgcgcct cgacaaggtc
     2041 ggcggcaagg gcctgttcgt gaaggagctc gagcaggcac tcgccgatgg ccgcgccgac
     2101 ctcgcggtgc actcgatgaa ggacgtgccc gtggcgctgc ccgcgggctt cgtgctcgcg
     2161 gcgacgctcg agcgcgagga tgcgcgcgac gcactggtga gcaacgctta cgcggagctc
     2221 gcggcgctgc cgcccggtgc gcgcgtcggc acctcgagcc tgcgccgcgc agcccaggtc
     2281 gccgcgcgct atccgcggct cgagctcgcg acgcttcgcg gcaacgtcga gacgcggctc
     2341 gccaagctcg accgcggtga gtacgacgcc gtgctgctcg cagccgccgg gctgaagcgc
     2401 ctcgggctcg aagcgcgcat ccggtgctgc ctgccggtcg acgagagcct gcccgcgccg
     2461 gggcaggcgg cgctcggcat cgagtgcctc gcctcgcgcg ccgacgtcgc ggcgctgctc
     2521 gcgccgctcg cgcacgccgc gagcgccgcc tgcgtgcgcg ccgagcgcgc ggtgagccgg
     2581 tcccttgccg gaagctgcca tgttccgctg gcggcatttg ccgtcatcga gggcgaaacc
     2641 ctctggctgc gcgggctcgt ggcgagtccg gacggcaagc aggtcgcgcg cggcgaattg
     2701 cgcgtgcccg tcgcggagcc cgagcgcgcg ggaagcgagc tcgcagcgct gctgcgaagc
     2761 cggggtgcgg acaagatact cgccgggct
//
LOCUS       DBNHKE010000011         7729 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_266765, whole genome shotgun sequence.
ACCESSION   DBNHKE010000011 DBNHKE010000000
VERSION     DBNHKE010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_266765"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1105)
                     /locus_tag="AC16O9_00605"
     CDS             complement(47..1105)
                     /locus_tag="AC16O9_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6515152.1"
                     /translation="MWIKGLGLLSAALATLAGCGAETNSKPAASAPRAHLVELAAAAP
                     GRLSYTADRAGSLRAVREVRLLNQEEGRLTEVRVREGDRVGAGEVLVRYDDRILRAEL
                     DKASATLNQAELDHERSRQLKEKGFVSDDAVSRAATALEIARAQVRLLETRVQRMTIA
                     APFAGVVARRLVEPGNVTPLHTHLLTVIDPSQLVTDVNVSELVMPYLSVGDKAEVRID
                     ALGEKLHPGKIVRIHPAIDPATRTGRVEVALYPVPPGARAGQFCRVVLSTGATEQLVV
                     PLAALQRDVDGEFVYVYQDTDGTVRRTGVASGLRLADRVEIRDGIERGAQVVVKGFLG
                     LSAGQRVKPVTPRAERSG"
     gene            1138..2025
                     /gene="cyoE"
                     /locus_tag="AC16O9_00610"
     CDS             1138..2025
                     /gene="cyoE"
                     /locus_tag="AC16O9_00610"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR6515153.1"
                     /translation="MTTLALPADFVQRARAFYALTKPRVVSLIVFTAVIGMFLAAPGM
                     VEPRVLLAGTFGIALVAGAAAAVNCLVEQNVDALMLRTRWRPLPSGELTSLQTLAFAG
                     VVGGLGLWLLAHFVNALTMWLTLATFVGYAVVYTVILKPLTPQNIVIGGASGAMPPVL
                     GWAAVTGEVTPEAMALFLIIFAWTPPHFWALALYRTEDYARAGLPMLPVTHGARYTRL
                     QVLLYTLILFGVSLLPFAMRMSGWLYLAAALALGGVFIAYALRIYFDYSDALARRTFR
                     YSIFYLALLFAALLVDHHL"
     gene            1994..2572
                     /locus_tag="AC16O9_00615"
     CDS             1994..2572
                     /locus_tag="AC16O9_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6515154.1"
                     /translation="MPRFWSIIICSLLAACDAGAPKFKSTDITGVEYGRTLALTDHHG
                     KARNLADFRGKAVVLFFGFVSCPDVCPTTLADMAQVMKKLGPEAARVQVLFVTVDPER
                     DTGAVLAKYVPAFHPDFLGLRGDLEATRAVAKEFKVYFEKRAGETPGSYSVDHSAQSY
                     VIDPQGRLRLFVRHERIAEDLAPDLRALLGAS"
     gene            2580..3461
                     /locus_tag="AC16O9_00620"
     CDS             2580..3461
                     /locus_tag="AC16O9_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR6515155.1"
                     /translation="MEFKDYYQTLGVGQDASADEIRKAFRKLARKYHPDVSKEAGAEA
                     RMKEVNEAYAVLSDPEKRAAYDQLGRGYQPGQEFRPPPDWDAGFEFSHHDMPGGDFSD
                     FFSELFGRMGARAHGQDHFAKVLLDVEDAFHGAERQIQLRVPQVDARGRVALETRTLN
                     VKIPKGVRAGQLIRLAGQGAGGDLLLEVHFKPHPRYRVEGRDLHVDLPVAPWEAALGA
                     VVRVEAPGGALNVRIPAGAQSGRVLRVRGKGIPAATPGDLLLAIRVVLPSADAPKARE
                     LYEQMARDLAFDPRAQP"
     gene            3458..3697
                     /locus_tag="AC16O9_00625"
     CDS             3458..3697
                     /locus_tag="AC16O9_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6515156.1"
                     /translation="MSDDELITLEELVVVCAVSREWVRERIDEGLLPERFDTLAVRRV
                     RRMRALERDFDAAPELAALMADLLEELDALRARLR"
     gene            complement(3694..4557)
                     /gene="rpoH"
                     /locus_tag="AC16O9_00630"
     CDS             complement(3694..4557)
                     /gene="rpoH"
                     /locus_tag="AC16O9_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR6515157.1"
                     /translation="MMSAQALPMALPMATGSLEAYIQTVSRLPLLTAEQEQDYARRYQ
                     REGDLEAARQLVLSHLRLVVAVARGYLGYGLPHADLIQEGNIGLMKAVKRFDPARGVR
                     LVSFAIHWVKAEIHEYILRNWRLVKVATTKAQRKLFFNLRSMKPGFATLSPGEVKRIA
                     SELKVKPEEVVEMEMRLAGREMAFEAPDDDEEVYAPAQYLADPDAEPSRLLEAEEEAR
                     VQTEGVARALATLDPRSRRIVEARWLKEKDTATLHDLAAEFGVSAERIRQIEAKAMQK
                     MKQVLALPAPA"
     gene            complement(4658..5542)
                     /gene="ftsX"
                     /locus_tag="AC16O9_00635"
     CDS             complement(4658..5542)
                     /gene="ftsX"
                     /locus_tag="AC16O9_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR6515158.1"
                     /translation="MRTWLRQHRAALGRAVRKLAAQRAATLLNALVIGVALSLPAGGY
                     ALLANLQGVAQRFTFEPQISVFLEPQAKRAELEPRLRADARVAALRFVPRDEALAELR
                     VAEGLADIVAALERNPLPDAFVVRAADGDAAALEALAHALKALPGVANVQVDSAWAER
                     LAALAKIGRLALGALAALLATGLVAVTFNTIRLQILTQREEIEVSQLLGATDAFIRRP
                     FFYQGFLQGLAGGGLALLILWAGLGVLNLGVRDLAQAYGSSFALAFLPGRDVLAVVAF
                     AAVLGWFGALLSVSKYLR"
     gene            complement(5539..6195)
                     /locus_tag="AC16O9_00640"
     CDS             complement(5539..6195)
                     /locus_tag="AC16O9_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR6515159.1"
                     /translation="MISFSAVSKRYPGGQDALAGVSFALAAGELAFVTGPSGAGKSTL
                     LKLIPAIERPTAGSVVVNGQNVGALKRGAIPYLRRNLGLVFQDHKLLYDRSVFDNVML
                     PLSFGALAPRDAARRARAALDKVGLLARERANPIQLSGGEQQRLAIARAVVNRPAVLV
                     ADEPTANLDADTAARVIDILLSFNQVGVTLVIATHDAALVARHGRRVLQLAAGRLVAA
                     "
     gene            complement(6188..7285)
                     /gene="ftsY"
                     /locus_tag="AC16O9_00645"
     CDS             complement(6188..7285)
                     /gene="ftsY"
                     /locus_tag="AC16O9_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6515160.1"
                     /translation="MQAHGAVEALAEPVEQRLAHAIGRWPHVCARGNVDTPPAPFAAH
                     NAHAARRSPLARIHRRMLAATSWTARLRSGLARTRAALGGGLGALFGRATVDEALYEA
                     LEEALLTADCGVAATRHLLERLRSRARRERITEPQALKRALQEELCLLLAPLERPLEL
                     ARAKPLVIMIAGVNGSGKTTSIGKLAKWLQAQGKSVLLAAGDTFRAAAREQLAAWGQR
                     NGVAVIAQQSGDAAAVVYDAAAAARARGIDALIADTAGRLPTQLHLMEEIKKVKRVLA
                     KALPGAPHEVLLVLDANTGQNALAQVKAFDEALGLTGLVLTKLDGTAKGGVVCAIARE
                     RPVPLLFIGVGEGIDDLRPFAAADFAEALVD"
     gene            7181..7702
                     /gene="rsmD"
                     /locus_tag="AC16O9_00650"
     CDS             7181..7702
                     /gene="rsmD"
                     /locus_tag="AC16O9_00650"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6515161.1"
                     /translation="MDVPARADVRPTPDRVRETLFNWLGQRLDGAVCLDLFAGSGALG
                     FEAASRGAARVVMVEADRAIAAALRKSRERLGAAAVEIVALDALAFLARGGERFDVVF
                     LDPPFRQNALPALLAALPPRLRRGARVYVEGERPAEAAAPWRELKRARAGQVSYQLLE
                     WSEHDPSGVPGDL"
BASE COUNT         1071 a         2756 c         2755 g         1147 t
ORIGIN      
        1 ccgggtggtg gatgcaccag accgcgatgc cgccgcggca catgcgctag cccgagcgct
       61 cggcgcgcgg cgtgacgggc tttacgcgct gcccggcgct aaggccgaga aagcccttca
      121 ccaccacttg cgcgccgcgc tcgatcccgt cgcggatctc gacgcggtcg gcgagcctga
      181 ggccgctcgc cacgccggtg cggcgcacgg tgccgtcggt atcctggtag acgtagacga
      241 actcgccgtc gacgtcgcgc tgcagcgcgg cgagcggcac gaccagctgc tcggtggcgc
      301 cggtggagag caccacgcgg cagaactggc cggcgcgcgc gcccggcggc accgggtaga
      361 gcgcgacttc gacgcgcccg gtgcgcgtcg ccggatcgat cgcgggatgg atgcgcacga
      421 tcttgcccgg gtgcagcttc tcgcccagcg cgtcgatgcg cacctcggcc ttgtcaccga
      481 ccgacaggta cggcatcacc agctccgaga cgttgacgtc ggtgaccagc tgcgaggggt
      541 cgatgacggt gagcaggtgc gtgtgcagcg gcgtgacgtt gccgggctcg accaggcgcc
      601 gcgccaccac accggcaaac ggggcggcga tggtcatgcg ctgcacgcgg gtctcgagca
      661 ggcgtacctg cgcgcgcgcg atctcgagcg cggtcgccgc gcgcgatacc gcgtcgtcgc
      721 tgacgaagcc cttttccttc agctgccggc tgcgctcgtg gtcgagctcg gcctggttca
      781 gcgtggcgct cgccttgtcg agctcggcac gcaggatgcg gtcgtcgtag cgcaccagca
      841 cctcgcccgc gccgacgcgg tcgccctcgc gcacgcgcac ctcggtgagg cgcccctcct
      901 cctggttgag cagacgcacc tcgcgcaccg cgcgcaggct gccggcacgg tcagcggtgt
      961 aggacaggcg cccgggcgcg gccgcggcca gctcgaccag gtgcgcgcgc ggcgcgctcg
     1021 cggcgggctt gctgttggtc tccgcgccgc agcccgcgag cgtggcgagc gcagcggaga
     1081 gcagcccgag tcccttgatc cacatgataa aattctacgc tttctgcggc cgccgtcgtg
     1141 accaccctcg cactccccgc cgacttcgtg cagcgcgccc gggccttcta tgccctgacc
     1201 aagccgcgcg tggtgtcgtt gatcgtgttc accgcagtga tcggcatgtt tctcgccgcg
     1261 cccggcatgg tcgagccgcg cgtgctgctt gccggcacct tcggcatcgc cctggtggcg
     1321 ggcgccgccg cggcggtgaa ctgcctggtg gagcagaatg tggatgcgct catgctgcgc
     1381 acgcgctggc ggccgctgcc gagcggcgag ctcaccagcc tgcagacgct cgctttcgcc
     1441 ggggtggtcg gcgggctcgg gctgtggctc ctggcgcact tcgtcaatgc gctcaccatg
     1501 tggctcacgc tcgccacgtt cgtcggctac gccgtcgtct acaccgtgat cctgaagccg
     1561 ctcacgccgc agaacatcgt catcggcggc gcttcgggcg cgatgccgcc ggtgctcggc
     1621 tgggccgcgg tgacgggcga ggtcacgccc gaggcgatgg cgctttttct catcatcttc
     1681 gcctggacgc cgccgcactt ctgggcgctg gcgctctacc gcaccgagga ctacgcgcgc
     1741 gccgggctgc cgatgctgcc ggtgacgcac ggcgcgcgct acacgcgcct gcaggtgctg
     1801 ctctatacgc tgatcctgtt tggcgtgtcg ctgctgccgt tcgccatgcg catgagcggc
     1861 tggctctacc tcgccgcggc gctggcgctg ggcggcgtgt tcatcgccta cgcactgcgc
     1921 atctacttcg actacagcga cgcgctcgcg cggcgcacct tccgctactc catcttctac
     1981 ctggcgctcc tctttgccgc gcttctggtc gatcatcatc tgtagcctgc tcgccgcctg
     2041 cgacgccggc gcgcccaagt tcaagagcac cgacatcacc ggcgtcgagt acggccggac
     2101 gctcgcgctg accgatcatc acggcaaggc gcgcaacctc gccgatttcc gcggcaaggc
     2161 agtggttctg ttcttcggct tcgtcagctg cccggacgtg tgcccgacga cgctcgccga
     2221 catggcgcag gtcatgaaga agctcggccc cgaggccgcg cgcgtgcagg tgctgttcgt
     2281 cacggtggac ccggagcgcg acacgggcgc ggtgctggcg aagtacgtgc cggcgttcca
     2341 tccggacttc ctcggcctgc gcggcgacct cgaggcgacg cgcgccgtag cgaaggagtt
     2401 caaggtgtac ttcgagaagc gagcgggcga gaccccgggg tcgtattccg tggaccacag
     2461 cgcgcagagc tacgtcatcg acccgcaggg gcggctgcgc ctgttcgtgc ggcacgagcg
     2521 cattgccgag gacctcgcgc cggacctgcg cgcgctgctc ggcgcgagct aggcgcggca
     2581 tggagttcaa ggactactac cagacgctcg gtgtcggcca agacgcctcg gcggacgaga
     2641 tccgcaaggc gttccgcaag cttgcgcgca agtaccaccc cgacgtgagc aaggaggcgg
     2701 gcgccgaggc gcgcatgaag gaggtgaacg aggcttacgc ggtgctctcc gaccccgaga
     2761 agcgcgccgc ctacgaccag ctcggccgcg gttatcagcc gggacaggaa ttccggccac
     2821 cgcccgactg ggacgcgggg ttcgagttct cgcaccacga catgccgggc ggggacttca
     2881 gcgacttctt ctcggagctg ttcggccgca tgggcgcgcg cgcacacggt caggatcact
     2941 tcgccaaggt gctgctcgac gtggaggatg ccttccacgg cgccgagcgc cagatccagc
     3001 tgcgcgtgcc tcaggtcgac gcgcgcggtc gcgtcgcgct cgagacgcgc acgctcaacg
     3061 tgaagatccc caagggggtg cgcgcaggcc agctcatccg cctcgcgggg cagggggcag
     3121 gcggcgacct gctgctcgaa gtgcacttca agccgcatcc gcgctaccgc gtcgaaggcc
     3181 gcgacctgca cgtcgacctg ccggtcgcgc cctgggaggc ggcgctcggc gcggtggtga
     3241 gggtcgaggc gccgggcggg gcgctcaacg tgcgcatccc ggcgggcgcg cagagcgggc
     3301 gcgtgctgcg cgtgcgcggc aagggcatcc ccgccgcaac gccgggcgac ctgctgctcg
     3361 cgatccgcgt ggtcctgccg agcgccgatg cgcctaaggc acgcgagctc tacgagcaga
     3421 tggcgcgcga cctcgctttc gatccacggg cccagccatg agcgacgacg agctgatcac
     3481 gctggaggag ctggtggtgg tgtgcgcggt gagccgcgag tgggtgcgcg agcgcatcga
     3541 cgagggcttg ctccccgagc gcttcgacac gctcgccgtg cgccgcgtgc ggcgcatgcg
     3601 cgcgctcgag cgcgacttcg acgcggcgcc cgagctcgcg gcgctgatgg ccgatctgct
     3661 ggaagagctc gacgcgctgc gcgcgcgcct gcgctaggcc ggcgcgggca gcgccagcac
     3721 ctgcttcatc ttctgcatcg ccttggcttc gatctggcgg atgcgctcgg ccgaaacgcc
     3781 gaactccgcg gcgaggtcgt gcagcgtggc cgtgtccttc tccttcagcc agcgcgcttc
     3841 gacgatgcgg cggctgcgcg gatcgagcgt ggcaagcgcg cgcgcgacgc cctcggtctg
     3901 cacgcgcgcc tcctcctcgg cttcgaggag acgcgaaggt tcggcgtcgg ggtcggcgag
     3961 gtactgcgcc ggggcgtaga cttcctcgtc gtcgtcgggg gcctcgaagg ccatctcgcg
     4021 gcccgccagg cgcatttcca tctcgacgac ttcctcgggc ttgaccttga gctcgctcgc
     4081 gatgcgcttc acctcgcccg ggctgagcgt ggcgaagccc ggcttcatcg agcgcaggtt
     4141 gaagaagagc tttctctgcg ccttggtggt ggccaccttc accagccgcc agttgcgcag
     4201 gatgtattcg tggatctcgg ccttcaccca gtggatggcg aacgacacca ggcgcacgcc
     4261 gcgcgccggg tcgaagcgct tcacggcctt catcaggccg atgttgcctt cctggatcag
     4321 gtccgcgtgc ggcaggccgt agccgaggta gccgcgcgcg accgccacca ccagccgcag
     4381 gtgcgacagc accagctggc gcgccgcctc gaggtcgccc tcgcgctggt agcgccgcgc
     4441 gtaatcctgc tcctgctcgg ccgtgagcag cggcaagcgg ctcaccgtct ggatgtacgc
     4501 ctcgagactg ccagtcgcca tcggcagcgc catcggcagg gcttgcgcag acatcatttg
     4561 cctccttggc cggatcttag cactcgcagt attggagtgc taagaagtcc caaggttccc
     4621 tgtgtgatgt ctgaaaccgt cttatcaaaa agcctattta gcgtaagtat ttactaactg
     4681 acagaagggc cccgaaccag ccgaggaccg ccgcgaaggc gacgacggcg agcacgtccc
     4741 ggcccggcag gaaggcgagc gcgaagctcg acccataggc ctgcgcaagg tcgcgcacgc
     4801 cgagattcag cactccgagc cccgcccaca ggatgagcag tgccaggccg ccgccggcga
     4861 gcccctgcag gaacccctgg tagaagaacg gccgccggat gaaggcgtcg gtggccccga
     4921 gcagttgcga cacctcgatc tcctcgcgct gcgtgaggat ctgcaggcgg atggtgttga
     4981 aggtcacggc gaccaggccc gtggcgagca gcgcggcgag cgcgccgagc gcgagccggc
     5041 cgatcttcgc cagcgccgcg aggcgctcgg cccaggccga gtccacctgc acgttcgcca
     5101 ccccgggcag cgccttgagc gcgtgcgcca gcgcctcgag cgccgccgca tcgccgtcgg
     5161 cggcgcgcac cacgaacgca tcgggcaggg ggttacgctc cagcgccgcg acgatgtcgg
     5221 cgaggccctc ggcgacgcgc agctcggcga gcgcctcgtc gcgcggcacg aagcgcagcg
     5281 ccgcgacgcg cgcgtcggcg cgcaggcgcg gctcgagctc ggcgcgcttt gcctggggct
     5341 cgaggaacac cgaaatctgc ggctcgaagg tgaagcgctg cgccacgccc tggaggttgg
     5401 cgagcagcgc gtagccgccg gcgggcagcg acagcgctac gccgatgacg agcgcgttga
     5461 gcagcgtcgc ggcgcgctgc gcggcgagct tgcgcacggc gcgcccgagc gccgcccggt
     5521 gctggcgcag ccaggtcctc atgccgcgac cagcctgccc gcggcgagct gcagcacgcg
     5581 gcgaccgtgg cgcgccacca gcgccgcgtc gtgcgtggcg atcaccagcg tgacgcccac
     5641 ctggttgaac gagagcagga tgtcgatcac gcgtgcggcc gtgtcggcat ccaggttggc
     5701 ggtgggctcg tcggccacca gcaccgccgg acggttgacc accgcgcgcg cgatcgccag
     5761 gcgctgctgc tctccgcccg aaagctggat cgggttggcg cgctcgcgcg cgagcagccc
     5821 caccttgtcg agcgccgcgc gcgcgcgccg cgccgcgtcg cgcggcgcca gcgccccgaa
     5881 cgagagcggc agcatgacgt tgtcgaagac gctgcggtcg taaaggagct tgtggtcctg
     5941 gaacaccagg ccgaggttgc ggcgcaggta ggggatcgcg ccgcgcttga gcgcgcccac
     6001 gttctggccg ttcaccacca cgctgcccgc ggtgggacgc tcgatcgccg gaatcagctt
     6061 caggagcgtc gacttgcccg cgcccgaagg cccggtgacg aaggcgagct cgcccgccgc
     6121 gagcgcgaac gacacgcccg cgagcgcatc ctggccgccc ggatagcgct tggaaaccgc
     6181 ggagaaacta atcaaccagc gcctcggcga aatccgcggc ggcgaagggc cgcaggtcgt
     6241 cgatgccctc gcccacgcca atgaacagca gcggcaccgg ccgctcgcgc gcgatcgcgc
     6301 acaccacgcc gcccttcgcg gtgccgtcga gcttggtcag caccagtccg gtaagcccga
     6361 gcgcctcgtc gaacgccttc acctgcgcga gcgcgttctg accggtgttg gcgtcgagca
     6421 ccagcagcac ctcgtgcggc gcgcccggca gcgccttcgc cagcacgcgc ttcaccttct
     6481 tgatttcctc catcaggtgc agctgcgtcg gcagtcgccc ggcggtgtcg gcgatcagcg
     6541 cgtcgatgcc gcgcgcccgc gctgcggccg ccgcgtcgta caccaccgcg gcggcgtcgc
     6601 ccgattgctg cgcgatgacg gcgacgccgt tgcgctgccc ccaggcggcg agctgctcgc
     6661 gcgccgccgc gcgaaacgta tcgccggcgg ccagcagaac ggacttgccc tgggcctgca
     6721 gccacttcgc gagcttgccg atggaggtgg tcttgcccga gccgttgacc cccgcgatca
     6781 tgatgacgag cggcttggca cgagcgagct cgagcggccg ttccagcggt gcgagcagca
     6841 ggcacagctc ttcctgaagc gcgcgcttga gcgcctgcgg ctcggtgatg cgctcgcgcc
     6901 gcgctcggct gcgcagccgc tcgagcaggt gccgcgtcgc ggcgacgccg cagtcggcgg
     6961 tgagcagcgc ttcttccagc gcttcgtaga gcgcctcgtc gaccgtggcg cggccaaaga
     7021 gcgcgccgag cccgccgccg agggcggcgc gggtgcgcgc aaggccgctt ctcaggcgcg
     7081 ccgtccacga agtcgctgct aacatcctgc ggtgaattct agcaagcggg ctgcgccggg
     7141 ccgcgtgcgc attgtgggcg gcgaatggcg cgggcggcgt atcgacgttc ccgcgcgcgc
     7201 agacgtgcgg ccaacgcccg atcgcgtgcg cgagacgttg ttcaactggc tcggccagcg
     7261 cctcgacggc gccgtgtgcc tggatctgtt cgcgggcagc ggcgcgctcg gcttcgaggc
     7321 cgcgtcgcgc ggcgcggcgc gcgtggtgat ggtggaggcc gaccgcgcca tcgccgccgc
     7381 gctgcgcaag agccgcgagc ggctcggcgc ggcggccgtg gagatcgtgg cgctggatgc
     7441 gctcgcgttc ctcgcgcgcg gcggcgagcg cttcgacgtg gttttcctcg acccgccgtt
     7501 caggcagaat gccctgccgg cgctgctcgc cgcgctgccg ccgcggctgc ggcgcggggc
     7561 gcgcgtgtac gtagaagggg agcggcccgc ggaagccgcg gcgccgtggc gcgagctaaa
     7621 gcgcgcgcgg gccgggcagg tgagctacca actactcgaa tggagcgaac atgatccgag
     7681 cggtgtaccc ggggaccttt gacccgctca cgcgcggcca tgaagacct
//
LOCUS       DBNHKE010000012         5977 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1271703, whole genome shotgun sequence.
ACCESSION   DBNHKE010000012 DBNHKE010000000
VERSION     DBNHKE010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1271703"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..416
                     /locus_tag="AC16O9_00655"
     CDS             207..416
                     /locus_tag="AC16O9_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515162.1"
                     /translation="MLKVSLPIAKARLIVRHALGAGRAANMMPLTVVVLDAGGHPVAL
                     EREDGSGILRSSPAQPAPDWAQSKL"
     gene            complement(426..502)
                     /locus_tag="AC16O9_00660"
     tRNA            complement(426..502)
                     /locus_tag="AC16O9_00660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Pro,seq:cgg)
     gene            complement(515..1492)
                     /locus_tag="AC16O9_00665"
     CDS             complement(515..1492)
                     /locus_tag="AC16O9_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6515163.1"
                     /translation="MPPTTASPLLAPVADDMRQVDALIRARLGSDVALVRQVAEYIVA
                     GGGKRLRPALLLLAAGALGCRGPACHELAAVVEFIHTATLLHDDVVDESRLRRGRRTA
                     NAAFGNAASVLVGDFLYSRAFQMMVGLDNLRVMRVLADATNTIAEGEVLQLMNCHDPD
                     VDEARYLDVVRRKTAKLFEAAARLGGILGGAAPALEQGLAAYGMHLGTAFQVVDDVLD
                     YSGAAQETGKSLGDDLAEGKPTLPLIRVMQAGSPEQRALVQRAIREGGREEFAPVLAA
                     IQSTRALDYARNAARREADAASRAIEALAPSAHRESLLELAAFAVSRRG"
     gene            1592..1909
                     /gene="rplU"
                     /locus_tag="AC16O9_00670"
     CDS             1592..1909
                     /gene="rplU"
                     /locus_tag="AC16O9_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6515164.1"
                     /translation="MYAIIKTGGKQYRVSAGEQVRVETLAADVGAAVSFDEVLLVGAG
                     DAVRVGAPLVSGAQVKATVVGHGRGDKVKIFKLRRRKHFQKSQGHRQNYTEVRIDDIV
                     AGA"
     gene            1930..2187
                     /gene="rpmA"
                     /locus_tag="AC16O9_00675"
     CDS             1930..2187
                     /gene="rpmA"
                     /locus_tag="AC16O9_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6515165.1"
                     /translation="MAHKKAGGSSRNGRDSQAKRLGVKVYGGEQVNAGQILVRQRGTV
                     VHPGSNVGMGRDHTLFARVAGKVQYLVKGERSKKTVCVQPA"
     gene            2251..3291
                     /gene="obgE"
                     /locus_tag="AC16O9_00680"
     CDS             2251..3291
                     /gene="obgE"
                     /locus_tag="AC16O9_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR6515166.1"
                     /translation="MKFIDEARIEVHAGKGGDGAAAFRREKYVPRGGPSGGDGGRGGS
                     VWAVADRNLNTLIDYRYARLHRAGDGAAGGGSDKYGRAGRDIELRVPLGTVLRDAESG
                     EPIADLARDGDRALLARGGRGGLGNLHFKSSINRAPRQYTRGEPGESRSLALELRVLA
                     DVGLLGLPNAGKSTFIRAVSSARPKVADYPFTTLNPSLGVVRIAHDASFVVADIPGLI
                     EGAAEGAGLGHQFLRHLARTRLLLHLVDIAPADEAAAPVPGVRAIVRELRKYDEALYR
                     KPRWLVFNKCDLAPDADERIARILHALRWTGPWFKVSALSGSGCRDVCNAAYRFLSAA
                     PRTTTTRARRAA"
     gene            3296..4423
                     /gene="proB"
                     /locus_tag="AC16O9_00685"
     CDS             3296..4423
                     /gene="proB"
                     /locus_tag="AC16O9_00685"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR6515167.1"
                     /translation="MTSPVAAAKTLVVKVGSSLVTNDGRGLDAAAIARWAAQIAALRK
                     LGRRCLLVSSGAIAEGLQRLGWTRRPHALHELQAAAAVGQMGLAQCYESCFREHGIAT
                     AQVLLTHADMADRQRYLNARFTLATLLELGVMPVINENDTVVTDEIKVGDNDTLGALV
                     ANLVEADALVILTDQAGLFEADPRRQPGARLVAQAQAGEARLVAMAGGTGSANARGGM
                     LTKVLAAQRAARSGAHTVIASGHEPEVLLRLARGEPVGTLLTAQTTPLAARKQWLADH
                     LTLGGRLELDAGAVRALAHDGKSLLPVGVTAVQGEFERGTVVGCLDPEGREVARGLVN
                     YSAAETRRIMRRPSSEIESILGYMDGPELIHRNNLVLLLAG"
     gene            complement(4420..4938)
                     /locus_tag="AC16O9_00690"
     CDS             complement(4420..4938)
                     /locus_tag="AC16O9_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HIR6515168.1"
                     /translation="MRALAGLALTLAGAAALAQPSDWERRQAEREWREIEVAPPAYPT
                     REHLLPFFVSAATSFEFFVDGTTLSVGADGVVRYVLVARSAQGAESVSFEGMRCSTGE
                     VRIYAVGTSARGWARSKSAWRQIKPREIQRWHNALFDEFFCPLGNPIHTSREGIDALK
                     RGGHPSRALSGN"
     gene            complement(4947..5531)
                     /locus_tag="AC16O9_00695"
     CDS             complement(4947..5531)
                     /locus_tag="AC16O9_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR6515169.1"
                     /translation="MMPRIERASLLSLEAYARERNAFRARVLEHKKHRRVQLGDHVTL
                     IFEDELTIRYQIQEMLRIERTFEEAGIQDELDAYNPLVPDGTNLKATMMIEYPDVAER
                     QAALARLKGIEDRVWVQVEGCARVHAIADEDLERENAEKTSAVHFLRFELDAEMRKAL
                     RYGVGLEIGVDHPQYQAAVEAAPAVRAALAADLA"
     gene            complement(5531..5950)
                     /locus_tag="AC16O9_00700"
     CDS             complement(5531..5950)
                     /locus_tag="AC16O9_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR6515170.1"
                     /translation="MELKGSSTEKNLKDAFAGESQANRRYLYFAKVADVEGYPEIAGN
                     FRETAEGETGHAHGHLEYLEQVGDPATGLPIGRTRDNLKSAVAGETHEYTDMYPGMAK
                     DARSEGFTEIADWFETLAKAERSHANRFQKALDALAD"
BASE COUNT          890 a         2122 c         2106 g          859 t
ORIGIN      
        1 gtgcacgccg cagtgcgcgc acgccacgag ctcgcccggc acgcccggcg cgcgcggcgg
       61 cgcgccgccc tggcgctcgc gcagcgcccg gcgtaccagc cagaccagga ccagcaccgt
      121 aagggcaagt aggaccaagc gtcccatggc gctattctag gagcttggtc aacgaagggt
      181 attcgacgag cgcacatccg aatccgatgc tgaaagtcag cctgccgatc gcaaaggcgc
      241 gcttgatcgt gcgccacgcg ctcggggcgg gccgcgcggc gaacatgatg ccgctcaccg
      301 tcgtcgtgct ggacgcgggc ggccatccgg ttgcgctcga gcgcgaggac ggctcgggca
      361 tcctgcgctc gtcgccggcg cagccggcgc cggactgggc gcaatcgaaa ctgtgagaag
      421 gggaatggtc ggggtgactg gattcgaacc agcgacacct gcgtcccgaa cgcagtactc
      481 taccaggctg agctacaccc cgaatcgccg gacactagcc gcggcgcgac accgcgaagg
      541 ccgccaattc tagcaaagat tccctgtgcg ccgagggcgc gagcgcctcg atcgcgcgcg
      601 atgccgcgtc ggcctcgcgc cgcgcggcgt tgcgcgcata gtcgagcgcg cgcgtcgact
      661 ggatcgccgc caggacgggc gcgaattcct cgcgcccgcc ctcgcggatg gcgcgctgca
      721 cgagcgcgcg ctgctcggga ctgcccgcct gcatgacgcg gatcagcggc agcgtcggct
      781 tgccctcggc caggtcgtcg ccgaggctct tgccggtttc ctgcgccgcg ccggagtagt
      841 cgagcacgtc gtcgaccacc tggaaggcgg tgcccagatg catgccgtag gcggccagcc
      901 cctgctccag ggccggcgcc gccccgccca ggataccgcc gaggcgcgcc gccgcctcga
      961 acagcttcgc ggtcttcctg cgcacgacgt cgaggtagcg tgcctcgtcc acgtccggat
     1021 cgtggcagtt catcagctgc agcacctcgc cctccgcgat ggtgttcgta gcgtcggcga
     1081 gcacgcgcat cacgcgcagg ttgtccagcc cgaccatcat ctggaaggcg cgcgagtaga
     1141 gaaaatcgcc gaccaggacc gaggcggcgt tgccgaacgc ggcgttggcg gtgcgccgac
     1201 cgcgccgcag ccgcgactcg tcgaccacgt cgtcgtgcag cagggtcgcg gtgtggatga
     1261 actccaccac cgcggcgagc tcgtggcagg ccggaccgcg gcacccgagc gccccggcgg
     1321 cgagcagcag cagggccggg cgcagccgct tgccgccccc ggcgacgatg tactcggcga
     1381 cctggcgcac cagggccacg tcggagccca ggcgcgcgcg gatgagcgcg tccacctgcc
     1441 gcatgtcgtc ggcaaccggg gcaagaaggg ggctggcggt ggtcggcggc atcggggcgc
     1501 tgcgcacgcg tagcatacaa ggctttgacg cgcgccggct gaatctccta taattccaag
     1561 tctttaccga actttggcgg cagaaccccc catgtacgca atcatcaaga ccggcggcaa
     1621 gcagtatcgc gtcagcgccg gcgagcaggt gcgcgtcgag acgctcgcgg ccgacgtcgg
     1681 cgcggcggtc tccttcgacg aagtgctgct ggtcggcgcg ggcgacgcgg tgcgcgtcgg
     1741 cgcgccgctc gtgtcaggcg cccaagtgaa ggccacggtc gtcggccacg gccgcggcga
     1801 caaggtcaag atcttcaagc tgcggcggcg caagcatttc cagaaatcgc agggccaccg
     1861 ccagaactac accgaggtcc ggatcgacga catcgtcgcc ggcgcctagc ggatcaggga
     1921 gaacggaaca tggcacacaa gaaagcaggc ggcagttcgc gcaacgggcg cgactcacag
     1981 gccaagcgcc tcggcgtgaa ggtttacggc ggcgagcagg tgaacgccgg ccagatcctg
     2041 gtgcgccagc gcggcaccgt ggtgcacccc ggctccaacg tgggcatggg acgcgaccac
     2101 accctgttcg cgcgcgtcgc aggcaaggtg cagtacctgg tcaagggcga gcgcagcaag
     2161 aagaccgtgt gcgtgcaacc ggcctgacgg ccggccaccc cacccaacca agccctatcg
     2221 gatccggtag ggctttttgc tttctgggcc atgaagttca ttgacgaagc gagaatcgag
     2281 gtgcacgccg gcaagggcgg cgacggcgcc gccgcgttcc ggcgcgagaa gtacgtgccg
     2341 cgcggcggcc cgagcggcgg cgacggcggg cgcggcggca gcgtgtgggc ggtggccgac
     2401 cgcaacctca acacgctgat cgactaccgc tacgcgcgcc tgcaccgcgc cggcgacggc
     2461 gccgcgggcg gcggctccga caagtacggc cgcgccgggc gcgacatcga gctgcgcgtg
     2521 cccctgggca cggtgctgcg tgacgccgag agcggcgagc cgatcgccga tctggcgcgc
     2581 gacggcgatc gtgcgctgct cgcgcgcggc ggccgcggcg gcctcggcaa cctgcacttc
     2641 aagtcgagca tcaaccgcgc gccgcgccag tacacgcgcg gcgagccggg tgagagccgc
     2701 agcctcgcgc tcgagctgcg cgtgctggcc gacgtgggcc tgcttggcct gcccaacgcg
     2761 ggcaagtcga ctttcatccg cgcggtatcg agcgcccgcc ccaaggtcgc ggattacccg
     2821 tttaccacgc tcaatccctc gctcggcgtg gtgcgcatcg cccacgatgc gagcttcgtg
     2881 gtggccgaca tccccgggct gatcgagggc gccgccgaag gcgccgggct cggccaccag
     2941 ttcctgcgcc acctcgcgcg cacgcgactg ctgctgcacc tggtcgatat cgcgccggcg
     3001 gacgaagcgg ccgccccggt gccgggggtg cgcgcaatcg tgcgcgagct gcgcaagtac
     3061 gacgaggcgc tgtaccgcaa gccgcgctgg ctggtgttca acaagtgcga cctcgcgccg
     3121 gacgccgacg agcgcattgc acgcatcctg cacgcgctgc gctggacggg cccctggttc
     3181 aaggtgagcg cactgagcgg gtccgggtgc cgcgacgtgt gcaacgccgc ctaccggttc
     3241 ctctcggcag cgccgaggac cacgacgacg cgcgcgcggc gcgccgcatg aggccatgac
     3301 cagcccggtc gccgccgcca agaccctggt cgtcaaggtc ggcagctcgc tcgtcaccaa
     3361 cgacggccgc gggctggacg cggccgcgat cgcgcgctgg gcggcgcaga tcgccgcgct
     3421 gcgcaagctc ggccggcgct gcctgctcgt ctcctcgggc gcgattgccg agggcctgca
     3481 gcgcctgggc tggacacggc gcccgcacgc gctgcacgag ctgcaggcgg cggcggccgt
     3541 gggccagatg gggctcgcgc agtgctacga atcgtgcttt cgcgagcacg gcatcgccac
     3601 tgcccaggtg ctgctgacgc acgccgacat ggccgatcgc cagcgctacc tgaacgcgcg
     3661 cttcacgctg gcgacgctgc tcgagctagg cgtgatgccc gtgatcaacg agaacgacac
     3721 cgtggtcaca gacgagatca aggtcggcga caacgatacg ctcggcgcac tggtcgccaa
     3781 cctggtggag gccgacgcgc tcgtcatcct gaccgaccag gcgggactgt tcgaggccga
     3841 tccgcggcgc cagcccggcg cgcgcctggt ggcgcaggca caggccggcg aggcgcgtct
     3901 cgtggcgatg gccggcggca ctggctcggc gaacgcgcgc ggcggcatgc tgaccaaggt
     3961 gctcgccgcg cagcgcgcgg cgcgcagcgg cgcgcatacc gtgatcgcct ccgggcacga
     4021 gcccgaggtt ctgctgcgcc tggcgcgcgg cgagcccgtc ggcaccctgc tcacggcgca
     4081 gaccacgccg ctcgccgcgc gcaagcagtg gctcgccgac cacctcacgc tgggcgggcg
     4141 cctcgagctt gacgccggcg cggtgcgcgc cctcgcgcac gacggcaaga gcctgctgcc
     4201 ggtgggcgtg actgcggtgc aaggcgagtt cgagcgcggc accgtggtcg gctgcctcga
     4261 tcccgagggg cgcgaagtgg cccgcggcct ggtgaactac tcggccgcgg aaacgcgccg
     4321 catcatgcgg cggccctcca gcgagatcga gtccatcctc ggctacatgg acgggcccga
     4381 gctgatccac cgcaacaacc tggtgctgct gctggcgggc tagttgccgc tcaaggcgcg
     4441 gctcggatgc ccgccgcgtt tgagggcgtc gatgccctcc cgcgaagtgt ggatcggatt
     4501 gcccagcggg cagaagaatt cgtcaaacag ggcgttgtgc cagcgctgga tctcgcgcgg
     4561 cttgatctgg cgccaggcgc tcttgctgcg cgcccagccg cgcgcgctgg tgccaacggc
     4621 gtagatgcgc acttcgccgg tgctgcagcg catgccctcg aagctcacgc tctccgcgcc
     4681 ctgcgcgctg cgcgccacga gcacgtagcg cactaccccg tccgcgccta cggacagcgt
     4741 cgtgccgtcg acaaagaact cgaagcttgt ggcggcactg acgaagaacg gcagtaggtg
     4801 ctcccgcgtg ggataggcag gcggcgccac ttcgatctca cgccactcgc gctcggcctg
     4861 ccggcgctcc caatccgagg gctgcgcgag cgcggcggcg cccgccagcg tcagcgccag
     4921 ccccgccagc gcccgcacgc cggcgttcag gcgagatcgg ccgcgagcgc cgcgcgcacg
     4981 gcgggcgcag cctcgacggc ggcctgatac tgcgggtggt cgacgccgat ctccaggccg
     5041 acaccgtagc gcagcgcctt gcgcatttcc gcgtccagct cgaagcgcag gaaatgcact
     5101 gccgaggtct tctccgcgtt ctcgcgctcg aggtcctcgt cggcaatggc atgcacgcgc
     5161 gcgcagcctt ccacctgcac ccacacgcgg tcctcgatcc ctttcagccg cgcgagcgcc
     5221 gcctggcgct cggcgacgtc cgggtactcg atcatcatcg tcgccttgag attggtgccg
     5281 tcaggtacca aggggttgta ggcgtcgagc tcgtcctgga tgccggcctc ctcgaaggtg
     5341 cgctcgatgc gcaacatctc ctggatctgg tagcggatgg tgagctcgtc ctcgaagatc
     5401 agcgtgacgt gatcgccgag ctggaccctg cgatgcttct tgtgctcgag cacccgcgcg
     5461 cggaacgcat tgcgttcacg cgcgtaggct tcgagcgaca gcaggctcgc gcgctcgatg
     5521 cggggcatca tcagtcggcg agggcgtcga gcgccttttg gaagcggttg gcgtgcgagc
     5581 gctcggcttt ggccagagtc tcgaaccagt ccgcgatttc cgtgaagcct tcgctgcgcg
     5641 cgtccttcgc catgcccgga tacatgtccg tgtactcgtg ggtctcgccc gccaccgccg
     5701 acttcagatt gtcgcgcgtg cggccgatcg gcaggccggt cgccgggtcg cccacctgct
     5761 cgaggtactc gaggtggccg tgcgcgtggc cggtctcgcc ttcggcggtc tcacggaagt
     5821 tacccgcgat ctccgggtag ccctccacgt cagccacctt ggcgaagtag aggtagcggc
     5881 ggttggcctg cgactcgccg gcgaacgcgt ccttgaggtt cttctcagtg ctgctgcctt
     5941 tcagttccat cgtgtctgtc tccttgctgt tggttgg
//
LOCUS       DBNHKE010000013        27932 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1377875, whole genome shotgun sequence.
ACCESSION   DBNHKE010000013 DBNHKE010000000
VERSION     DBNHKE010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 27932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1377875"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /gene="argA"
                     /locus_tag="AC16O9_00705"
     CDS             complement(<1..912)
                     /gene="argA"
                     /locus_tag="AC16O9_00705"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR6515171.1"
                     /translation="MSAPLKPEDFVRWFRQTAPYLHAFGGRTFVIGFGGELIAEHARF
                     AQFVQDVNLLAALDIRLVLVHGARPQIEAELKAKGLRSRYAKGLRVTDAAALTAVKHA
                     AGALRVEIEALLSQGLPSSPMAGAEIRVASGNYIAAQPIGVLDGLDHQFTGLVRKVDA
                     TAIARRLEAGEVVLVPHIGYSPTGEVFNLSWEDVAQSVASALRADKLLLFVDRLPADR
                     KGAVIGELTAREAEALLKKADLGVSATRALRHALAALAAGVGRAHLISRRTPGAALLE
                     LFTHSGVGTMITAEPMQRLRQARIEDVG"
     gene            complement(937..1680)
                     /locus_tag="AC16O9_00710"
     CDS             complement(937..1680)
                     /locus_tag="AC16O9_00710"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR6515172.1"
                     /translation="MHEKNARVARFYTEAALRAGGVSLLPEDAAHHAVHVLRLRPGEE
                     VVLFNGRGGEYAGRIAAVDRLRVSVDLLEHRRLERESPLAVTLVQGVSAGEKMDFTVQ
                     KATELGVAALQPVLAARSTGRISGERAALKRAHWQRVAVAACEQCGRNRIPEVLPVVP
                     LAQLCAAAPAPGTRLLLSPLATLGLRAAKLEGAVALAAGPEAGFTAEEEALLAATGFL
                     PVRLGPRVLRTETAALAALAALNALAGDF"
     gene            1694..2047
                     /locus_tag="AC16O9_00715"
     CDS             1694..2047
                     /locus_tag="AC16O9_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6515173.1"
                     /translation="MKRPWHATTREAAAVPLPPGRRSAEILRNGSLEVRWYAPRGADP
                     QTPHGRDEVYVVAAGRAAFVRGAERVAVAAGDLLFVPAGMAHRFEEMSGDFGTWVMFY
                     GPAGGERDNPFASRP"
     gene            2044..3081
                     /locus_tag="AC16O9_00720"
     CDS             2044..3081
                     /locus_tag="AC16O9_00720"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6515174.1"
                     /translation="MKISGLFGTELPIIQAPMAGAQAGAMAIAVSNAGGLGSLPCALL
                     SAEAMREELGAIKAHTARPYNVNFFCHAPPRPSAEREAAWRKALAPFYKEYGIDPESI
                     PAGSGRAPFSAEFADALEPFRPPVVSFHFGLPSNDLLARVKGWGAKILGSATTVDEAR
                     WLEARGVDAIIAQGLEAGGHRGIFLTDDLTTQVGTFALLPQVVRAVKLPVIAAGGIAD
                     AQGVAAALALGAAGVQIGTAYLLCPEATTGAVHRAALKSDAARHTALTNLFTGRPARG
                     IVNRVMKELGPISGAAPEFPLATSAIMPLRAKAEARGSGDFTSLWSGQNAAGCREVPA
                     AQLTRELAAGI"
     gene            complement(3152..3250)
                     /locus_tag="AC16O9_00725"
                     /pseudo
     CDS             complement(3152..3250)
                     /locus_tag="AC16O9_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029107.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3275..3862)
                     /locus_tag="AC16O9_00730"
                     /pseudo
     CDS             complement(3275..3862)
                     /locus_tag="AC16O9_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3853..4869)
                     /locus_tag="AC16O9_00735"
     CDS             complement(3853..4869)
                     /locus_tag="AC16O9_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR6515175.1"
                     /translation="MNGRARAIAYAASALVLLLLAGLAFALGRFPVSPGELAQLAANK
                     LFGAGYSVAPSAETVVLQVRGPRILAALLVGAALAAAGTAYQGMFRNPLVSPDILGVS
                     AGAALGAVLGIFFSQSALVIQLGAFAGGLAAVGVVYAVGANVRRHDPILSLILAGIVL
                     GTLLGAFIALIKYLADPYNQLPAITYWLLGSLAAVAPSDLALAAPFALAGLVPLYLLR
                     WRMNLLSLSDDEARALGVPVARLRLAVVCAATLMTAAAVAISGIIGWVGLLIPHAARM
                     LIGPEFSRLLPLSMLLGAAFLLAVDTLARTAAPIEIPPGVLTALIGTPFFLWLFVSAR
                     RAWQ"
     gene            complement(4866..6386)
                     /locus_tag="AC16O9_00740"
     CDS             complement(4866..6386)
                     /locus_tag="AC16O9_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR6515176.1"
                     /translation="MLQAADLTIRLHPDDDVVIARVEIPAGTRVQKEGVRAEAMIPAG
                     HKLATRAVEAGKPVRRYNQVIGFATQTIRPGDHVHVHNLAMGEFERDYAFCAGVKPID
                     YAQPSATFLGIVRADGRVATRNYIGILTSVNCSATVARMVADHFRNNLADYPNVDGVV
                     ALTHKTGCGMASEGEGMELLRRTLTGYAQHPNFYATVSIGLGCEANQLNTWMGSAKLK
                     VNEKLQAFTIQEKGGTTKAVKDGIARIKALLPEANKVKREPVPASHLILGLECGGSDG
                     YSGISANPALGAAADLLVRHGGTPILSETPEVYGAEHLLTRRAVSREVGEKLLTRIRW
                     WEDYTRRSNMEMNNNPSPGNKAGGLTTILEKSLGAAAKGGTTNLVDVYQFAEKVTAKG
                     FVFMDTPGYDPVSVTGHVAGGDNVVCFTTGRGSVYGCKPSPSLKLATNTRLYQHMEED
                     MDVNCGTIVDGTETVEQVGERIFRLILETASGKRTKSELLGFGEDEFAPWVVGATM"
     gene            complement(6397..8130)
                     /locus_tag="AC16O9_00745"
     CDS             complement(6397..8130)
                     /locus_tag="AC16O9_00745"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HIR6515177.1"
                     /translation="MAKKKPIPLRSVQWFGRQDIYGFIYRSWTKNRGIPQDQFDGRPV
                     VGICNTYSELVPCNSHFRTIAEHVKAGVLEAGGFPLEFPVMSLGETLLRPTAMLYRNL
                     ASMDVEESIRANPLDGVVLLVGCDKTTPSLLMGAASVDLPTVAVSGGPMLSGKYRGTD
                     IGSGTNTWSMSEELRAGKITLEEFHEAESCMHRSHGHCMTMGTASTMASMVESLGVGM
                     PGNAAIPAVDARRNVLARMAGRRAVEMVKENLRLSKILTKQAFENAIRVNAAIGGSTN
                     AVIHLIAIARRIGVDLKLADWDRLGRGVHTLVNLMPSGKYLMEDFYYAGGLPVVIREL
                     GEAGKLHKNALTVNGRSLWDNNKDAQCWNRDVIHSFKKPFKKDTGIAVLRGNLCPDGA
                     IIKPSAATPKLLKHRGRAVVFENIEDFHKRIDDPNLDIDAKCIMVLKNCGPKGYPGMA
                     EVGNMPLAPKLLKKGITDMVRISDARMSGTAYGTVVLHVAPEAAAGGTLALVENGDMI
                     ELDVAKRRLQLLVSPKELARRRAKWNKPKAPLARGYWKLYFDHVLQADKGADLDFLVG
                     SSGGFVPRDNH"
     gene            complement(8132..8836)
                     /locus_tag="AC16O9_00750"
     CDS             complement(8132..8836)
                     /locus_tag="AC16O9_00750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515178.1"
                     /translation="MNQLDLKGRTAIVTGGLIGIGAAIVKRLEASGAKVRIWDVGDKS
                     NPVDVTDQKAIERATAKALADLGKIDILVNNAGIAGPTMPVVEYPVDEWKRVIDIDLN
                     GPFLCCRAVVPHMVKAKYGRIVNIASIAGKEGNPTAAAYAAAKGGVIAFTKALGKELA
                     KTGVLVNCITPAAAQTAILDQVTPEFAQFMLSKIPMGRFVTVDEIAAMACWLATEDCS
                     FTTAGVFDISGGRATY"
     gene            complement(8833..9726)
                     /locus_tag="AC16O9_00755"
     CDS             complement(8833..9726)
                     /locus_tag="AC16O9_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6515179.1"
                     /translation="MADAKRALPGGDESEGMSYLSTLPRRLVTLYLPLSLIVVVLLFP
                     FYWMTVTAVKPKEHLLDLDTHSPFWTVSPTLEHINDLLFKTDYPLWLWNTMFIAVSAT
                     ILSLIASVFAAYAIVRIRYRGAQTAGGLIFLAYLVPPSILFIPLSTVVFQYGLFDTPF
                     ALILTYPTILIPFSTWLLMGYFKTIPYELEECALIDGATRWQILWKIVLPLAVPGLIS
                     AAIFCLTLCWNEFIYALTFINSTEKKTVPVAILSAFTDGDQYRWGSLMAGALVGSLPL
                     VILYAFFVEHYVSAMTGAVKE"
     gene            complement(9719..10630)
                     /locus_tag="AC16O9_00760"
     CDS             complement(9719..10630)
                     /locus_tag="AC16O9_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6515180.1"
                     /translation="MKLSWTELRMNRNWLGFWFMLPAAAFLIAFLVYPLGLGIWLSFT
                     DARIGTPGKFIGLENYEWIIGDSKFQTAVFFTLFYTIVASIVKFALGLYLALLLNQNM
                     PFKAIMRAIILIPFIVPTVLSAIAFWWIYDPQFSIISWSLKQLGWIEGNIDFLGDPWN
                     ARWSVIFANIWRGVPFIAISLLAGLQTVPPSLYEAATLDGATPWQMFVRITYPLLTPI
                     IAVVMTFSVLFTFTDFQLIKVLTNGGPAGATELMATMSYNTAILGSRIGEGAAVSTAM
                     VPFLLAAIMVSWFGLQRRKWQQGEGDG"
     gene            complement(10651..11991)
                     /locus_tag="AC16O9_00765"
     CDS             complement(10651..11991)
                     /locus_tag="AC16O9_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6515181.1"
                     /translation="MTDRKTSRRDVLKGSAALAGAAALQASHLNLFAQAWAQSSPWKP
                     EKGAKLQLLRWKRFVQSEEDGFMALVAAFTKATGVQVTVLNESLDDVQPKASVAANTG
                     QGPDLFWGLYSLPHLFPAKCMDVTDVANYLGKKYGGWVASAQVYGRSGNKWIDIPVAY
                     NGNVINYRQSMIEKAGFKTIPQDTAGFMELMKALKEKSQPGGFALGRASGDGNAWVHW
                     CLWAFGGNLVDAKDKVIINSPETAKALEYAKQLSSHFAPGTAAWNDAFNNKAFLSSEI
                     SLTNNGVSIYAAAKAGAAKGEAKMKEIVDDMNHSLWPVGPVGKPTEFHIAYPMLAMKY
                     TKVPNACKAFMAFTMEAQNYNPWLEAAVAYLTHPLNAYDSNPVWTSDPKLSIVRDVAK
                     RTLTAGHLGSVGEKAASALADFVILDMFATVCTDRASVKDAMAVAERQARRIYR"
     gene            complement(12041..13105)
                     /locus_tag="AC16O9_00770"
     CDS             complement(12041..13105)
                     /locus_tag="AC16O9_00770"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515182.1"
                     /translation="MAAVAISAARKSFGHTEVLHGVDIAIADGEFCVLVGPSGCGKST
                     LLRMIAGLENITAGEIRIGERVVNNVHPKERDIAMVFQNYALYPHMTVADNMGFSLAL
                     RGTAKALIKERVGVAADILGLAPYLDRYPRQLSGGQRQRVAMGRAIVRNPQVFLFDEP
                     LSNLDAKLRVAMRTEIKELHQRLKTTTVYVTHDQIEAMTMADKIVVMHDGRVEQMGDP
                     LTLYDRPANRFVAGFIGSPAMNFLEGAVAGADGRRVVNMASGARLPVAEAGAATEGQP
                     VIYGIRPEHLELADDGFDAEVIVIEPTGSETQLFARVGGQEIVAVFRERHEFAPGQKI
                     RLRPRPDMAHLFDPSSGDRI"
     gene            13282..14022
                     /locus_tag="AC16O9_00775"
     CDS             13282..14022
                     /locus_tag="AC16O9_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6515183.1"
                     /translation="MAGRLTGKTAFITAAAQGIGRGAALAFAREGAKVWATDVNAKAL
                     ADLEGKDGIRTRVLDVLDEQAIGKAAAEAGPIDVLFNCAGYVHHGTILECTPKDWDFS
                     FNLNVKSMYLVTRAFLPGMLKQGRGSIINMSSIASSVKGLPNRFVYGATKAAVVGLTK
                     AIAADYVKQGIRCNCIGPGTVDTPSLGERISAFADPVQARKDFIARQPMGRLGTVEDI
                     TGILVFLASDESLFATGNMYSIDGGMTI"
     gene            14019..14867
                     /locus_tag="AC16O9_00780"
     CDS             14019..14867
                     /locus_tag="AC16O9_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6515184.1"
                     /translation="MKLCRYGKDGFEKPGMIDGEGKLRDLSKVVAHIGPNELSPRGLK
                     MLTKVKPESLPVVSNSPRLGVPYVGIGKFVAIGLNYRDHAIESNLPIPSEPVVFSKAT
                     SCINGPSDNIIQPKGSTKLDWELELGVVIGTKAQYVSEASALEYVAGYCVVNDVSERS
                     FQHATSQWDKGKGFDTAGPIGPWLVTTDEVKDPQALDMWLDVNGKRMQSGNTRTMIFS
                     VAQIVSHVSQYMTLLPGDIITTGTPPGVGAGIKPNPVWLKPGDVITAGIRGLGEQKQK
                     VVAWPG"
     gene            complement(14875..15633)
                     /locus_tag="AC16O9_00785"
     CDS             complement(14875..15633)
                     /locus_tag="AC16O9_00785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515185.1"
                     /translation="MNPFDLKGKTALVTGGNGGIGLGIAKGLVQAGAKVAIAGRNEAK
                     NQAAVKALGSDAIALQVDVNDEAAVKRMVADTVKALGGLDILVANAGINIRKPPQDYT
                     MEEWHRIMDTNLSSVFACCQAAYPEMKKRGGGKIVTIGSMTSIFGFDVGPVYAASKGA
                     VVQLTKSLASAWAKDNIQVNSILPGFIDTDLTRNLRRNFPEAHERVVARTPARRWGEP
                     ADLAGTAVFFCSRASDFVTGTALPVDGGFSSSMF"
     gene            15678..16928
                     /locus_tag="AC16O9_00790"
     CDS             15678..16928
                     /locus_tag="AC16O9_00790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HIR6515186.1"
                     /translation="MDKLRINADRLWGSLMELAQIGATPKGGVRRLTLTEEDRRARDL
                     FARRAREAGLAVDVDAIGNIFARRAGADPSLPPVAMGSHLDSQPSGGKFDGAYGVMAG
                     LEVLRTLNDAGVATRAPMEVVSWTNEEGSRFQPVMMGSGVFANVFSLEHVRAQKDLDG
                     VAVGDALQAIGYAGAARPHRLGAYFEAHIEQGPVLENENKTIGVVQGALGQRWYDVTV
                     TGQDAHAGPTPMELRRDALIGASRLVLEVNRIARTFPDYARGTVGFMQVQPNSRNVIP
                     GEVRTSVDFRNAKAATLDAMCEELKRAARVIESECRVLIELRETVYFRPCEFDAGLVA
                     GVREAASALGYSHRDIVSGAGHDAVYVARVAPTAMIFIPCEGGISHNEIESAAPADLT
                     AGGNVLLHAALRKAGIAGAARAAA"
     gene            16939..18267
                     /gene="aptA"
                     /locus_tag="AC16O9_00795"
     CDS             16939..18267
                     /gene="aptA"
                     /locus_tag="AC16O9_00795"
                     /EC_number="2.6.1.-"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0016223 - beta-alanine:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omega-aminotransferase AptA"
                     /protein_id="HIR6515187.1"
                     /translation="MNDRATLPSLENSAFFMPFSMNRHFKKAPRLLARAEGVYYYTPE
                     GRKLIDGSSGLWCVNAGHCREKIVKAVQKQVAELDFAPPFNMGHPLAFEFADKVASLA
                     PADLKRVFFTNSGSESVDTALKLALAYHRVRGEGHRVRLIGRERGYHGVNFGGTAVGG
                     MVANRKIFGALVAGVDHIRSTHDLDRNAYSRGQPKHGAEFADELERLCALHDPSTIAA
                     VIVEPVACSGGVLVPPVGYLERLRAICDKHGILLIFDEVITGWGRLGKTFGTQHFNVI
                     PDMITTAKGITNGTVPMGAVLMRERLYQAFMVGPEKAIEVPHGYTYSSHPLACAAGLA
                     TMQVYEEEGLFTRAAELWPYWEEQAHALKGLPNVIDIRNIGLIAAIELTPRKDAPGAR
                     ALDAHIAAFERGAYMRVAADTIALSPPLIITKSEIDQLFEIVRGVLKSTD"
     gene            18289..19176
                     /locus_tag="AC16O9_00800"
     CDS             18289..19176
                     /locus_tag="AC16O9_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR6515188.1"
                     /translation="MFGFGKNKDEAPASEAPDDRAAGGGLSSNRTRIALLALVLVAGL
                     GYLAYEYLPGLLDPAPPPMPVQAKPKPKPAPVAVAQTTAVPVPVAAAVTKPQAEPAPE
                     PKAKPAPTPEPAAKPEPKPVPKPAAKPAPKPEPIAKPESKPAPVVVAAAPAAEPKPAA
                     EPKPAAEPKPAAEPTPAPALVAPAAAVAAAVAPLRGPPPIVLKYNDVMTAVLYSDREA
                     VAQLLDVGRWVDKPDSNGLTPLMAAVLGRDPDMVRLLLERGADPNLQAPGGEVALEMA
                     RANGDGASESLLLKAGARY"
     gene            complement(19178..20437)
                     /gene="speE"
                     /locus_tag="AC16O9_00805"
     CDS             complement(19178..20437)
                     /gene="speE"
                     /locus_tag="AC16O9_00805"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR6515189.1"
                     /translation="MNGLHLIGDLSGCRCDPQLLLDGAGFKDKCLEFVRDAGLTTMDA
                     SFHQFDGSGFTGTVVLAESHLAIHTWPEKQGLTLDVYVCNYSDDNSAKARKLFDAIVA
                     HFQPAECARHEVERGGHLVMEPLNDSTGFYVKAARQVGEWQTRFQRLQIFDTPHYGKI
                     LRLDGYNMTSEKEEFVYHENLIHPALTAHPAPKKVLIIGGGDGGSSEEALKHPSVEQV
                     TMCEIDPDVIRVAKEHFRAVHNGVFDNPKLRVLVGDGMKFVRETQERFDLIALDLNDP
                     MGPAEALYSAEFFQQCRQALAPGGALTLHLGSPVAQPERVGELAQRLNGIYRIVRPYI
                     MYIPLYGSLWSMAVCSDKLDPKAYTADEIDRRIEQRKMTELRYYNGETHEGVFALPNF
                     VRDLVTPPRLKQQTRGRRLGVVRAAAK"
     gene            complement(20607..21494)
                     /gene="nudC"
                     /locus_tag="AC16O9_00810"
     CDS             complement(20607..21494)
                     /gene="nudC"
                     /locus_tag="AC16O9_00810"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR6515190.1"
                     /translation="MLLLPFSSPRAFEHRVHGAGDAEGLWFVFRGDRLLVEVGPPDPR
                     PGDDPRVRERPSWARLPLRKNNTPLWSPPLRTLYLGGLEGLDCWAAEVADAQLPPGHD
                     WVGLRALFSVLEDAHFALAGRALQLLQWDRDHQFCGRCGTRTEPQAEERVRRCPACGL
                     SAYPRVAPAVMALVRRGRELLLGRSPHFPAGMYSALAGFVEPGESLEQCVAREVAEEV
                     GVTLGDMSYFASQPWPFPHSLMIAFVCEWAGGEIRPQEGEIEAASWFDVLQLPKLPSK
                     ISIARRLIDAVSAEIRRQI"
     gene            21521..23527
                     /gene="tkt"
                     /locus_tag="AC16O9_00815"
     CDS             21521..23527
                     /gene="tkt"
                     /locus_tag="AC16O9_00815"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR6515191.1"
                     /translation="MNPPASDRELADAIRALAMDAVEAAKSGHPGMPMGMAEIAVALW
                     QRHLRHNPANPAWANRDRFVLSNGHGSMLLYALLHLTGYDLPMAELRRFRQLGSKTPG
                     HPELGCAPGVETTTGPLGQGVSNAVGMAIAERLLAAEFNRPGFDVIDHYTYVFLGDGC
                     MMEGVSHEACSLAGTLGLGKLIAVYDDNGISIDSDKGQIRQWYTDDVPKRFAAYGWQV
                     IANVDGHDVEAMDKALKKAKREKSRPSLICAKTVIAKGAPKKANTGAAHGAPLGAEEV
                     AATRQALGWPHAAFEIPQAIYDGWDARAAGKRAERRWTKLVAAYEKQFPAEAAELRRR
                     TAGTLPADFAARIAALTAELHAKAESVATRKASQAVLEALVPALPELVGGSADLTGSN
                     LTMVKASRVVGREGGGNYLFYGVREFGMCAVMNGLALHGGVLPYGGTFLVFSDYARNA
                     LRLAALMRQRVIYVFSHDSIGLGEDGPTHQPVEHAASLRLIPYMDVWRPCDAAETAMA
                     WAVALERRDGPSALLLSRQNVAPAPRAAEALELARRGGYVLSDAPWAKAVLIATGAEV
                     PIALEAQKRLAAADLPVRVVSMPSTTVFDRQDAGYREGVLPPALPRVAVEAGVTDFWR
                     KYVGLQGAVVGLDRFGESAPAGDLFKHFGFTAENVEKAVRSVIP"
     gene            23524..24540
                     /gene="gap"
                     /locus_tag="AC16O9_00820"
     CDS             23524..24540
                     /gene="gap"
                     /locus_tag="AC16O9_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6515192.1"
                     /translation="MTIKVAINGFGRIGRNILRAHYEGKKKHALQFVAINDLGDPATN
                     AHLLKYDTAHGRFPGRVAVEGDAIAVEGDKLKVLAERDPAKLPWKALGVDVVLECTGL
                     FTGKAKGGAAHVSAGAKKVIISAPGDKDVDRTIVMGVNHASLKASDTVISNASCTTNC
                     LAPLVKALDDRIGVVSGLMTTVHSFTNDQVLTDVYHKDLRRARSATHNMIPTKTGAAA
                     AVGLVLPHLNGKLDGYAIRVPTINVSIVDLTFVAKKATTVDAVNQALQQAAQTDLKGI
                     LEYTSEPLVSCDFNHHPASSIVDGALTKVADGTLVKVASWYDNEWGFSNRMLDAAVAL
                     MSAK"
     gene            24546..25700
                     /locus_tag="AC16O9_00825"
     CDS             24546..25700
                     /locus_tag="AC16O9_00825"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6515193.1"
                     /translation="MRFKRMADLDLRGKRVFIRADLNVPLEAGRITDDTRIRASVPGI
                     RLALEKGAAVMVTSHLGRPKEGELKPEDSLAPVAQRLAELLGREVPLKRDWVEGVEVK
                     PGEVALLENCRVNMGEKKDDEALAKKIAALCDVYVNDAFGTAHRAEATTHGAARFAKV
                     ACAGPLMAAELDALGKALAHPERPLVAIVAGSKVSTKLTVLGERAKKVDQLIVGGGIA
                     NTFILASGLPVGKSLVEKDLVDEARRIMQATRVPLPEDVVVGRSLEDTRGVTKAARDV
                     GADEMILDIGPKTAAAYAGTLAGAGTILWNGPVGVFENNAFSRGTETLARAVAASGAF
                     SIAGGGDTVAAINKFGMAEGVSYISTAGGAFLEFLEGKTLPAVAALEARA"
     gene            25734..27173
                     /gene="pyk"
                     /locus_tag="AC16O9_00830"
     CDS             25734..27173
                     /gene="pyk"
                     /locus_tag="AC16O9_00830"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR6515194.1"
                     /translation="MLRSTKIVATLGPASSDPAVLERMLRAGVDVVRLNFSHGTADDH
                     RKRAELVRDIGRKIERTVGIMCDLQGPKIRIGKFRDGPVTLETGARFVLDAACAEGNA
                     ERVGLDYKELPRDVEEGAVLLLDDGKIVLEVTAVRGDEVQTRVRHGGVLSNNKGINRQ
                     GGGLTAPALTPKDMEDIRTAAQLHADFLAVSFPKSGADMYMARELMRAAGGKAMLIAK
                     IERAEAVKHEALLSIMAACDGIMVARGDLAVEVGDAAVPALQKRMIRLAREHNKLTIT
                     ATQMMESMISSPVPTRAEVSDVANAVLDGTDAVMLSAESASGKYPVQTIEAMDRICVE
                     AEQTQSISLDQDFLNRVFTRVDQSIAMGALFTAFHLKVKAIAALTESGATALWMSRLN
                     CGVPIYALTSQTSTRYRCSLLRDTYPLMVKYVGHDREELLREAEKVLVDNGVVKNGDL
                     IVLTIGEPIGKAGGTNTMKIVRVGEHLRA"
     gene            27196..27648
                     /locus_tag="AC16O9_00835"
     CDS             27196..27648
                     /locus_tag="AC16O9_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6515195.1"
                     /translation="MTAITPKVAAHATLAPLASRFVEVAKLPWEDTRFPGVRQKVLVV
                     DRDSGVATVLIHMAPGAKLPDHEHVLLEQTYVLEGSLVCGEGECKAGDFVWRPAGSRH
                     SAWGGPQGGTMLAIFQIANRFFDPDGRETDVNGQDWEAAWGKTLFREA"
     gene            complement(27698..>27932)
                     /locus_tag="AC16O9_00840"
     CDS             complement(27698..>27932)
                     /locus_tag="AC16O9_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6515196.1"
                     /translation="GYAVAQTMEQVLKQAGNDLSRANIMKQAANLKDFRPTVLLPGIR
                     INTSPTDFAPIESEQLARFDGEGWVRFGEIIGK"
BASE COUNT         4503 a         9495 c         9705 g         4229 t
ORIGIN      
        1 gccgacgtcc tcgatgcgcg cctgccgcag ccgctgcatg ggctcggcgg taatcatggt
       61 gccgacgccc gagtgcgtga agagctcgag cagtgcggcc ccgggtgtgc ggcgcgagat
      121 caggtgcgcg cggccgacgc cggcggcaag cgcggcgagg gcgtggcgca aggcgcgcgt
      181 ggcgctcacg ccaagatcgg ccttcttcag cagcgcctcg gcctcgcgcg ccgtgagctc
      241 gccgatcacc gcgcccttgc gatccgcggg cagccggtcg acgaacagga gcagcttgtc
      301 cgcgcgcagc gcgctggcga cgctctgcgc cacgtcttcc caggacaggt tgaacacctc
      361 gccggtgggc gaatagccga tatgcggcac cagaaccact tcacccgcct cgagccgccg
      421 cgcgatggcg gtggcgtcga ccttgcgcac caggccggtg aactggtggt ccaggccgtc
      481 gagtacgccg atcggctgcg cggcgatgta gttgcccgag gcgacgcgga tctccgcacc
      541 ggccatcggc gagctcggca agccctgcga cagcagggcc tcgatctcca cgcgcagcgc
      601 ccccgccgcg tgcttcacgg cggtcaacgc cgccgcgtcg gtgacgcgca ggcccttggc
      661 gtagcgcgag cgcagcccct tcgccttcag ctcggcctcg atctgcgggc gcgccccgtg
      721 caccagcacc agccggatgt cgagtgcggc gagcaggttc acgtcctgga cgaactgcgc
      781 aaagcgcgcg tgctccgcga tcagctcgcc gccgaagccg atcacgaacg tgcgcccgcc
      841 aaaggcgtgc aggtagggcg cggtctgccg gaaccagcgg acgaagtctt cgggcttgag
      901 gggcgcgctc atcggctcgc gattctaagg caaggctcag aaatcgcccg cgagcgcgtt
      961 caacgccgcg agcgcggcga gcgccgcggt ctcggtgcgc agcacgcgcg ggcccaggcg
     1021 tacgggaagg aagccggtcg cggcgaggag cgcttcctcc tcggcggtga agcccgcttc
     1081 gggccccgcc gcgagcgcca ccgcgccctc gagcttcgcg gcgcgcagtc cgagcgtggc
     1141 gagaggcgag agcagcaggc gggtgcccgg ggcgggcgcc gccgcacaga gctgcgccag
     1201 cggcacgacc ggcagcacct cgggaatgcg gttgcgcccg cattgctcgc acgcggcgac
     1261 cgccacgcgc tgccagtggg cgcgcttcag cgcggcgcgc tcgccgctga tgcggccggt
     1321 cgagcgcgcg gcgagcaccg gctgcagtgc ggcgacgccc agctcggtgg ccttttgcac
     1381 ggtgaaatcc atcttctcgc ccgccgacac cccctgcacc agcgtcacgg cgagcggcga
     1441 ctcgcgctcg agccgccgat gctcgagcag atccaccgac acgcgcagcc ggtcgaccgc
     1501 ggcgatgcgt cccgcgtact cgccgccacg gccattgaac aggaccacct cttcgccggg
     1561 ccgcaggcgc agcacgtgca ccgcgtgatg cgccgcgtct tcgggcagca gcgaaacgcc
     1621 tccggcacgc agcgcagctt cggtgtagaa gcgcgctacg cgcgcatttt tttcatgcat
     1681 actcggattc tgcatgaagc gtccctggca cgcgacgacg cgcgaagcgg cggcggtccc
     1741 gctgccgccg ggccggcgct cggccgagat cctgcgtaac ggcagcctcg aagtgcgctg
     1801 gtacgcgccg cgcggggcgg atccgcagac gccgcacggg cgcgacgagg tctatgtggt
     1861 ggcggccgga cgcgccgcgt tcgtgcgcgg cgccgagcgc gttgcggtgg cggcaggcga
     1921 cctgctgttc gtgccggcgg gcatggcgca ccgattcgag gagatgagcg gggacttcgg
     1981 cacctgggtg atgttctacg gcccggccgg cggcgagcgc gacaatccct tcgcgagccg
     2041 accttgaaga tatccggact tttcggcacc gagctgccga tcatccaggc accgatggcg
     2101 ggggcgcagg cgggcgcgat ggcgatcgcc gtgtccaatg ccggaggact tggttcactg
     2161 ccttgcgccc tgctctcggc cgaggcgatg cgcgaggagc tcggggccat caaagcgcac
     2221 accgccaggc cctacaacgt gaacttcttc tgccacgcgc cgccgcggcc gagcgccgag
     2281 cgcgaggccg cctggcgcaa ggcgctggcg cccttctaca aggaatacgg catcgatccc
     2341 gagtcgattc cggcgggatc cggaagagcg ccgttcagcg cggagttcgc cgacgcgctg
     2401 gagccgttcc gcccgccggt agtgagcttc catttcggct tgccctcgaa cgacctgctg
     2461 gcgcgcgtca agggctgggg cgcgaagatt ttgggctccg cgacgacggt cgatgaagcg
     2521 cgctggctgg aagcgcgggg tgtggacgcg atcatcgcgc agggcctgga agcgggcggg
     2581 catcgcggca tctttctcac cgacgacctg acgacgcagg tgggcacgtt cgcgctgctg
     2641 ccgcaggtcg tgcgcgcggt caagctgccc gtgatcgccg ccggcggcat tgccgacgcg
     2701 caaggcgtgg cggcggcgct ggcgctgggc gcggcgggcg tgcagatcgg caccgcctat
     2761 ctgctgtgtc cggaagccac caccggcgcc gtgcaccgcg cggcgctgaa gagcgacgcc
     2821 gcacgccaca ccgcgctcac caacctgttc accggtcgcc cggcacgcgg catcgtgaac
     2881 cgcgtaatga aagagctcgg cccgatcagc ggcgcggcgc cggaatttcc gctggcgaca
     2941 tcggcaatca tgccgttgcg cgccaaggcg gaggccaggg gctccggcga tttcacgtcg
     3001 ctgtggtccg ggcagaacgc ggccggctgc agggaagtgc cggcggcgca gctcacgcgg
     3061 gagctggcgg cggggatcta ggccgtgccg cctacagcac cggcaggcac acgctcgtgc
     3121 cgtcgagcgg cgcgacgcgc acttcgaccc cgtacacctg gcgcaggttc tccgccgtga
     3181 tcacctccgc cggcgtgccc tggcgcagcg ccccgccgcg cgcgaggagc agcacgcgac
     3241 tcgcggcgag gaacgcctgg ctcgggtcgt gcgaggagaa gacgaccgcg tagccctcgg
     3301 cggcgaggcg ccggatctcg cgcagcacgc gcacctggtt gccgaaatcc aggctcgccg
     3361 tgggctcatc gagcaccagc gcgcgggcgc cttgcgccag cgcgcgcgcg atcatggtga
     3421 gctggcgctc gccgccggag agctcggtca ccacgcgctc ggcgagctcg ccgatgccga
     3481 gcgtctcgat ggcgcgcgcg gcggccgcgc ggtcggccgc gccaggcgcg gcgaagaggc
     3541 cgatgtgcgc cgtgcgcccc atcagcacca catcgcgcac ggtgaaagcg aactgcgcga
     3601 cgtgcccctg cggcacgtag ccgacgcggc gcgcgacttc gctacggcct agggcggcga
     3661 gcgcctcgcc ctcgagccgc acttctcctt cctgcgccga cagcagcccg agcaacgtgc
     3721 ggaacagcgt cgtcttgccg ccgccgttcg ggccgagcac gcacagcacc tcgcccgcgc
     3781 cgagcgcgag ctccaggtcg cggcccacag ggcgcccggg gtagccatag cccagcttgc
     3841 gcgcctcgag cgtcactgcc atgcgcgccg cgccgagacg aagagccaca ggaagaacgg
     3901 cgtgccgatg agcgcggtga gcacgcccgg cgggatctcg atcggcgccg ccgtgcgcgc
     3961 cagcgtgtcc acggcgagga ggaacgccgc gccgagcagc atcgagagcg gcagcaggcg
     4021 cgagaactcc gggccgatca gcatgcgcgc cgcatgcggg atgagcagtc ccacccaccc
     4081 gatgatcccc gagatcgcca ctgccgcagc ggtcatcagc gtcgcggcgc acaccaccgc
     4141 gaggcgcagg cgcgccaccg gcacgccgag cgcgcgcgcc tcgtcatccg acaacgacag
     4201 caggttcatg cgccagcgca ggagatacag tggcaccagc cccgcgaggg cgaacggcgc
     4261 agcgagcgcg aggtccgaag gcgcgaccgc ggcaaggctg ccgagcagcc agtaggtgat
     4321 cgccggcagc tggttgtacg ggtcggccag gtacttgatg agcgcgatga acgcgccgag
     4381 cagcgtgccg aggacgatgc cggcgaggat cagcgacagg atcgggtcgt ggcggcgcac
     4441 gttggcgccg accgcgtaga ccacgcccac cgcggcaagc ccgcccgcga aggcgccgag
     4501 ctggatgacg agcgcgctct gcgagaagaa gatgccgagc accgcgccga gcgcggcgcc
     4561 cgccgagacg ccgaggatgt cgggcgagac cagcggattc ctgaacatgc cctgataggc
     4621 ggtgccggcg gcggcgagcg ccgcgcccac cagcagcgca gcgaggatgc gcggcccgcg
     4681 cacctgcagg accacggttt cggcggaggg ggcgacgctg tagccggcgc cgaagagctt
     4741 gttcgccgcg agctgcgcga gctcgccggg cgaaaccggg aagcgcccga gggcaaatgc
     4801 gagccccgcg aggagcagca ggaccagcgc cgaagcggcg tacgcgatag cgcgcgcgcg
     4861 cccgttcaca tcgtggcgcc gacgacccag ggcgcgaact cgtcctcgcc gaatcccagc
     4921 agctcggact tggtgcgctt gcccgaggcg gtctccagga tcagccggaa gatgcgctcg
     4981 ccgacctgct ccacggtttc ggtgccgtcg acgatggtgc cgcagttcac gtccatgtcc
     5041 tcttccatgt gctggtagag gcgcgtgttg gtggcgagct tgagcgaggg cgagggcttg
     5101 cagccgtaca ccgagccgcg gccggtagtg aagcacacca cgttgtcccc gcccgccacg
     5161 tgcccggtga ccgacaccgg gtcgtagccg ggcgtatcca tgaagacgaa gcccttggcc
     5221 gtcactttct cggcgaactg gtagacgtcg acgaggttgg tcgtgccgcc cttggccgcc
     5281 gcgccgagcg acttctccag gatcgtggtg aggccgcccg ccttgttgcc gggcgagggg
     5341 ttgttgttca tctccatgtt gctgcgacgg gtgtagtcct cccaccagcg gatgcgcgtg
     5401 agcagctttt cgcccacttc ccgcgacacc gcgcggcgcg tgagcaggtg ctcggcgccg
     5461 tagacctccg gtgtctccga gaggatcggc gtgccgccgt ggcgcaccag caggtcggcc
     5521 gctgcgccga gcgccgggtt ggcgctgatc ccggagtagc cgtccgagcc gccgcattcg
     5581 agcccgagga tcaggtggct cgcgggcacc ggctcgcgct tgaccttgtt cgcctccggc
     5641 agcagcgcct tgatgcgcgc gatgccgtcc ttcaccgcct tggtggtgcc gcccttttcc
     5701 tggatggtga aggcctgaag cttttcgttg accttcagct tcgcgcttcc catccaggtg
     5761 ttgagctggt ttgcctcgca gcccaggccg atgctcaccg tggcatagaa attcgggtgc
     5821 tgcgcgtagc cggtgagcgt acggcgcagc agctccatgc cctcgccctc ggaggccatg
     5881 ccgcagccgg tcttgtgcgt gagcgccacc acgccgtcga cgttgggata gtcggcgagg
     5941 ttgttcctga agtgatcggc caccatgcgc gcgacggtcg ccgagcagtt gacgctcgtc
     6001 aggatgccga tgtagttgcg cgtggccacg cgcccgtcgg cgcgcacgat gcccaggaag
     6061 gtggcgctcg gctgcgcgta gtcaatgggc ttcacgccgg cgcaaaacgc gtagtcgcgc
     6121 tcgaactcgc ccatggcgag gttgtgcacg tgcacgtgat cgcccgggcg gattgtctgg
     6181 gtggcgaagc cgatcacctg gttgtagcgg cgcaccggct tgcccgcttc gaccgcgcgc
     6241 gtggcgagct tgtgcccggc cgggatcatg gcctccgcgc gcacgccctc tttctgcacc
     6301 cgcgtgcccg cgggaatctc cacccgcgcg atgacgacgt catcgtctgg gtgcaggcgg
     6361 atggtgaggt cagcggcctg cagcatgcga gcctcctcag tgattgtcgc gaggtacgaa
     6421 gccgccgctg ctgccgacga ggaaatcgag gtccgcgccc ttgtcggcct gcagcacatg
     6481 atcgaagtac agcttccagt agccgcgcgc gagcggcgcc ttgggcttgt tccacttcgc
     6541 gcggcgcctg gcgagctcct ttggcgaaac gaggagctgc aggcggcgct tggccacgtc
     6601 gagctcgatc atgtcgccgt tctccaccag cgcgagcgta ccgcccgcgg cggcctcggg
     6661 cgccacgtgc agcaccacgg tgccgtaggc ggtgccgctc atcctcgcgt ccgagatgcg
     6721 caccatgtcg gtgatgccct tcttcaggag cttgggcgcg agcggcatgt tgccgacctc
     6781 ggccatgccc gggtagccct tcggcccgca gttcttcagc accatgatgc acttcgcgtc
     6841 gatgtcgagg ttggggtcgt cgatgcgctt gtggaagtcc tcgatgttct cgaataccac
     6901 ggcgcggccg cggtgcttga gcagcttcgg cgtcgcggcc gagggcttga tgatcgcgcc
     6961 gtcggggcag aggttgccgc gcagcaccgc gatgcctgtg tccttcttga acggcttctt
     7021 gaaactgtgg atcacgtcgc ggttccagca ctgcgcgtcc ttgttgttgt cccacagcga
     7081 gcggccgttc acggtgagcg cgttcttatg cagcttgccg gcttcgccca gctcgcggat
     7141 caccaccggc aggccgcccg cataatagaa atcctccatc aggtacttgc ccgagggcat
     7201 gaggttgacg agggtgtgca cgccgcgccc gaggcggtcc cagtccgcga gcttgaggtc
     7261 caccccgatg cgccgcgcga tggcgatcag gtggatcacc gcgttggtcg agccgccgat
     7321 cgcagcgttg acgcggatcg cgttctcgaa cgcctgcttg gtgaggatct tggagagccg
     7381 caggttttcc ttcaccatct ccaccgcgcg ccgcccggcc atgcgcgcga gcacgttccg
     7441 gcgcgcgtcc accgcgggaa tcgcggcgtt gcccggcatg ccgacgccga gggattcgac
     7501 catcgaggcc atcgtggaag ccgtgcccat cgtcatgcaa tgcccgtgcg agcggtgcat
     7561 gcacgactcg gcctcgtgga actcctccag cgtgatcttg cccgcgcgca gctcttccga
     7621 catcgaccag gtgttggtgc ccgagccgat gtcggtgccg cggtacttgc ccgagagcat
     7681 cggcccgccc gagaccgcca ccgtgggcaa gtcgacgctc gccgcgccca tgagcagcga
     7741 cggcgtggtc ttgtcgcagc ccaccagcag caccacgccg tccagcggat tggcgcggat
     7801 cgactcctcg acgtccatgc tcgcgaggtt ccggtagagc atcgccgtgg gccgcagcag
     7861 cgtctcgccg agcgacatca ccgggaactc gagcggaaag cccccggcct ccaatacccc
     7921 cgcctttacg tgctcggcga tggtgcggaa gtgcgagttg caggggacga gctcggaata
     7981 ggtgttgcag atgccgacca ccggccggcc gtcgaactgg tcctgcggaa tgccgcggtt
     8041 cttggtccag ctgcggtaga tgaagccgta gatgtcctgg cgcccgaacc actggacgct
     8101 gcgcaacgga atgggtttct tcttcgccat gtcagtaggt cgctcttccg cccgagatgt
     8161 cgaacacgcc cgcggtggtg aacgagcagt cctcggttgc cagccagcac gccatcgccg
     8221 cgatttcgtc caccgtgacg aaacggccca tcgggatctt ggagagcatg aactgcgcga
     8281 actcgggcgt cacctggtcg aggatcgcgg tctgcgccgc cgcgggcgtg atgcagttga
     8341 cgagcacgcc ggtcttggcg agttccttgc cgagcgcctt ggtgaaggcg atgacgccgc
     8401 ccttggcggc ggcgtaggcg gcggcggtgg ggttgccttc cttgccggca atcgaggcga
     8461 tgttgacgat gcgtccgtac ttcgccttca ccatgtgcgg caccacggcg cggcagcaca
     8521 ggaacgggcc gttcaggtcg atgtcgatga cgcgcttcca ctcgtccacc gggtactcga
     8581 ccacgggcat ggtggggccc gcgatgccgg cgttgttgac caggatgtcg atcttgccga
     8641 gatcggccag ggctttggcg gtggcgcgct cgatcgcttt ctggtcagtg acgtccaccg
     8701 ggttggactt gtcgccgacg tcccagatgc gcactttggc gcccgaggcc tcgaggcgct
     8761 tgacgatcgc ggcgccgatg ccgatcagac ctccggtaac gatcgcggtg cggcccttca
     8821 ggtcgagctg gttcattcct tgacggctcc ggtcatggca gatacgtaat gttcgacgaa
     8881 gaacgcgtag aggatgacga gcggcagcga accgacgagc gcgcccgcca tcagcgagcc
     8941 ccagcggtac tggtcgccgt cggtgaaggc ggacaggatc gccaccggca cggtcttctt
     9001 ctcggtcgag ttgatgaagg taagcgcgta gatgaactcg ttccagcaca gcgtcaggca
     9061 gaagatcgcc gccgagatca gcccggggac ggcgagcggc agcacgatct tccacaggat
     9121 ctgccagcgc gtcgcgccgt cgatcagcgc gcattcctcg agctcgtacg ggatggtctt
     9181 gaagtagccc atcaagagcc acgtcgagaa cgggatcaga atcgtcggat aggtgaggat
     9241 cagcgcgaag ggcgtgtcaa agaggccgta ctggaacacc accgtcgaga gcggaatgaa
     9301 caggatcgac ggcggcacca ggtaggcgag gaagatgagg ccgcccgcgg tctgcgcgcc
     9361 gcggtagcgg atgcgcacga tggcgtaggc tgcgaacacg ctcgcgatga gcgacaggat
     9421 ggtcgccgac accgcgatga acatggtgtt ccagagccac agcgggtagt cggtcttgaa
     9481 cagcaggtcg ttgatgtgct cgagcgtcgg actgacggtc cagaacgggc tgtgcgtgtc
     9541 gaggtcgagc aggtgctcct tgggcttcac cgcggtcacc gtcatccagt agaacgggaa
     9601 cagcagcacc accacgatca acgacagggg caggtacagc gtcaccagcc gccgcggcag
     9661 cgtcgacaga tagctcatgc cctcggattc gtcgccgccg ggcagcgcgc gtttcgcgtc
     9721 agccatcgcc ctctccctgt tgccatttgc ggcgctgcag gccgaaccag gacaccatga
     9781 tcgcggcgag caggaacggc accatcgccg tggagaccgc cgcgccctcg ccgatgcgcg
     9841 agccgaggat cgcggtgttg taggacatgg tggccatcag ctcggtggcg ccggccgggc
     9901 cgccgttggt gagcaccttg atgagctgga agtcggtgaa cgtaaagagc acggagaagg
     9961 tcatcaccac cgcgatgatc ggcgtgagca gcgggtaggt gatgcgcacg aacatctgcc
    10021 agggcgtcgc gccgtcgagc gtggcggctt catacagcga cggcggcacg gtctgcagcc
    10081 cggcgaggag cgatatcgcg atgaagggca ccccgcgcca gatgttggcg aagatcacgc
    10141 tccagcgcgc attccacgga tcgcccagga agtcgatgtt gccctcgatc cagccgagct
    10201 gcttcagcga ccacgagatg atcgagaact gcgggtcgta gatccaccag aaggcgatcg
    10261 ccgagagcac cgtgggcacg atgaacggga tcaggatgat cgcccgcatg atcgccttga
    10321 acggcatgtt ctggttcagc aacagcgcca ggtacagccc gagggcgaac ttcacgatgc
    10381 tggcgacaat ggtatagaac agcgtgaaga acaccgccgt ctggaatttc gagtcgccga
    10441 tgatccactc gtagttctcc aggccgatga acttgcccgg cgtgccgatg cgcgcgtcgg
    10501 tgaacgacag ccagatgccc aggccgagcg ggtacaccag gaacgcgatg agaaaggcgg
    10561 cggcaggcag catgaaccag aacccgagcc agttccggtt catgcgcagc tcggtccagc
    10621 tgagcttcat gcctgccccc gcgcgccgcg ttagcggtag atgcgccgcg cctggcgctc
    10681 ggccactgcc atcgcgtcct tgacgctcgc ccggtcggtg cagaccgtgg cgaacatgtc
    10741 gaggatcacg aagtcggcca gcgccgaggc ggccttttcg ccgaccgaac cgaggtgccc
    10801 cgcggtcagc gtgcgcttgg caacgtcgcg aacgatggac agcttcgggt ccgaggtcca
    10861 gaccggattc gagtcgtagg cattgagcgg gtgcgtgagg taggccaccg ccgcctcgag
    10921 ccaggggttg tagttctggg cctccatggt gaaggccatg aacgctttgc acgcgttcgg
    10981 caccttggtg tacttcatcg ccagcatcgg gtaggcgata tgaaactcgg tcggcttgcc
    11041 gaccgggccc acgggccaga gcgagtggtt catgtcgtcc acgatttcct tcatcttggc
    11101 ttcgcccttc gcggcgcccg ccttggcggc ggcgtagatc gacacgccgt tgttggtgag
    11161 cgagatctcg ctcgacagga aggccttgtt gttgaaggcg tcattccagg ccgcggtgcc
    11221 gggcgcgaaa tgcgacgaga gttgcttggc gtactcgagc gccttggcgg tctcgggcga
    11281 attgatgatc accttgtcct tggcgtcgac gaggttgccg ccgaaggccc acaggcacca
    11341 gtgcacccag gcgttgccgt cgcccgaggc gcggcccagc gcaaacccgc ccggctggct
    11401 cttctctttg agcgccttca tcagctccat gaagcccgcg gtgtcctgcg ggatcgtctt
    11461 gaagccggcc ttctcgatca tgctctggcg gtagttgatg acattgccgt tgtaggccac
    11521 cggaatgtcg atccacttgt tgcccgacct gccgtacacc tgcgcgctcg ccacccagcc
    11581 gccgtacttc ttgccgaggt agttggcgac gtcggtgacg tccatgcact tggccggaaa
    11641 caggtgcggc agcgaataca ggccccagaa gaggtcgggc ccctggccgg tgttcgcggc
    11701 cacggaagcc ttgggctgca cgtcatcgag cgactcgttc agcaccgtga cctgcacgcc
    11761 ggtcgccttg gtgaacgcgg cgaccagcgc catgaagccg tcttcttcgg actgcacgaa
    11821 gcgcttccag cgcagcagct gcagcttggc gcccttttcc ggcttccacg gcgagctctg
    11881 tgcccaggcc tgggcgaaaa ggttgagatg ggacgcctgc aacgcggcgg cgccggcgag
    11941 cgccgcgctg cccttcagta cgtcgcggcg cgaggttttg cggtcggtca tgtgcgtttc
    12001 tccagttggt ggttcgcgtg caagtaacct cgggcggcgg ctagatgcgg tcgccgctcg
    12061 acggatcgaa taggtgcgcc atgtcgggac gcgggcgcag gcggatcttc tgccccggcg
    12121 caaactcgtg gcgctcgcgg aacaccgcga cgatttcctg cccgccgacg cgcgcgaaga
    12181 gctgcgtctc cgagccggtc ggctcgatca cgatcacttc ggcgtcgaag ccgtcgtcgg
    12241 cgagctcgag gtgctcgggg cggatgccat agatgaccgg ctggccctcg gtggcggcgc
    12301 cggcctcggc caccggcagg cgcgcgccgg aggccatgtt gaccacgcgc ctgccgtccg
    12361 caccggccac ggcgccttcg aggaagttca tcgccggcga gccgatgaag ccggcgacga
    12421 agcggttggc cggccggtcg tagagcgtga gcggatcgcc catctgctcg acgcgcccgt
    12481 cgtgcatgac gacgatcttg tcggccatcg tcatcgcctc gatctggtcg tgcgtgacgt
    12541 acacggtggt ggtcttgagg cgctggtgca gctccttgat ctcggtgcgc atcgccacgc
    12601 gcagcttggc gtcgaggttc gagagcggct cgtcgaacag gaacacctgc gggttgcgca
    12661 cgatggcgcg gcccatggcg acgcgctggc gctggccgcc ggagagctgg cgcggatacc
    12721 ggtcgaggta cggcgcgagg ccgaggatgt ccgcggcgac ccctacgcgc tccttgatca
    12781 gcgccttcgc ggtgccgcgc aaggccagcg aaaagcccat gttgtcggcc accgtcatgt
    12841 gcgggtagag cgcatagttc tggaacacca tggcgatgtc gcgctctttc gggtgcacgt
    12901 tgttgaccac gcgctcgccg atgcggatct cgcccgcggt gatgttctcc aggcccgcga
    12961 tcatgcgcag cagcgtggac ttgccgcagc ccgaggggcc gaccaggacg cagaactcgc
    13021 cgtcggcgat cgcgatgtcg acgccgtgca gcacctcggt gtgaccaaag gacttgcgcg
    13081 cggcgctgat ggcgacggcg gccatgctat ttccccacct ccatcgggcg attctaccct
    13141 cggcgcgtcg cggccgacgc caaagttaac ccggcggtta cgcgaatcgg tcgggcgggg
    13201 gatgcgaacc gcccggctcc ccggcgccgg ttgcatttat aatcgccccc ctgctctggg
    13261 agaggaacaa gagcacgacg catggcaggc agacttaccg ggaagaccgc cttcatcacc
    13321 gcagccgcgc agggcatcgg ccgtggcgct gcgctcgcgt tcgcgcgcga aggcgcgaaa
    13381 gtgtgggcca ccgacgtcaa cgccaaggcg ctggccgacc tcgaaggcaa ggacggcatc
    13441 cgcacgcgcg tgctcgatgt gctcgacgag caggcgatcg gcaaggcggc cgccgaggcc
    13501 gggccgatcg acgtgctgtt caactgcgcc ggctacgtgc accacggcac gatcctcgag
    13561 tgcacgccca aggactggga cttcagcttc aacctcaacg tgaagtcgat gtacctcgtg
    13621 acgcgcgcct tcctgcccgg gatgctgaaa cagggcaggg gctcgatcat caacatgtcc
    13681 tcgatcgctt ccagcgtgaa gggcctgccc aaccgctttg tctacggcgc aaccaaggcc
    13741 gcggtggtcg gcctcaccaa ggcgatcgcc gccgactacg tgaagcaggg catccgctgc
    13801 aactgcatcg ggccgggcac cgtggacacg ccgtcgctcg gcgagcgcat cagcgcgttc
    13861 gccgacccgg ttcaggcgag gaaggatttc atcgcccgcc agccgatggg gcggctcggc
    13921 acggtggagg acattacggg gatcctggtg ttcctcgcct ccgacgagtc gctgttcgcg
    13981 acgggcaaca tgtactccat cgacgggggg atgacgatat gaagctgtgc cgctacggca
    14041 aggacgggtt cgagaagccg ggcatgatcg acggcgaggg gaagctgcgc gacctgtcca
    14101 aggtggtcgc gcacatcggc ccgaacgagt tgtcgccgcg cgggctgaag atgctgacca
    14161 aggtgaagcc cgaatcgctg ccggtggtct ccaacagccc gcgcctgggc gtgccctacg
    14221 tagggatcgg caagttcgtc gccatcgggc tgaactatcg ggaccacgcg atcgagtcca
    14281 acctcccgat cccttccgaa ccggtggtgt tctcgaaggc gaccagctgc atcaacggac
    14341 cgagcgacaa catcatccag cccaaggggt ccaccaagct cgactgggag ctcgagctcg
    14401 gcgtggtgat cggcaccaag gcccagtacg tgtcggaagc gagcgcgctc gagtacgtcg
    14461 cgggctactg cgtcgtcaac gacgtctcgg agcgatcgtt ccagcacgcc accagccagt
    14521 gggacaaggg caagggcttc gacaccgccg ggccgatcgg cccgtggctc gtcaccaccg
    14581 acgaagtgaa ggacccgcag gcgctcgata tgtggctcga cgtgaacggc aagcgcatgc
    14641 agtcgggcaa cacacgcacc atgatcttca gcgttgcgca gatcgtgtcg cacgtcagcc
    14701 agtacatgac gctgctgccg ggagacatca tcaccaccgg cacgccgccg ggtgtcggcg
    14761 cgggcatcaa gcccaacccg gtgtggctca agccgggcga cgtgatcacc gccggcatcc
    14821 gcggcctggg cgagcagaag cagaaggtcg tcgcctggcc gggctgagcg ccggctagaa
    14881 catcgagctg gagaagcccc cgtccaccgg cagcgcggtg ccggtgacga agtcgctcgc
    14941 ccggctgcag aagaacaccg ccgtgccggc aaggtccgcc ggttcacccc agcgccgcgc
    15001 cggcgtgcgc gcgacgacgc gctcgtgcgc ctcagggaag ttgcgccgca ggttgcgcgt
    15061 cagatcggtg tcgatgaagc cgggcaggat gctgttgacc tggatgttgt ccttcgccca
    15121 ggcggacgcg aggctcttgg tcagctgcac caccgcgccc ttgctcgccg cgtacacggg
    15181 gccgacgtcg aagccgaaga tgctggtcat cgagccgatg gtgacgatct tgccgccgcc
    15241 gcgcttcttc atctcgggat aggccgcctg gcagcacgcg aacaccgatg acaggttggt
    15301 gtccatgatg cggtgccact cttccatcgt gtagtcctgc ggcggcttgc ggatgttgat
    15361 gcccgcgttc gcaacgagga tgtcgagccc gccgagggcc ttgaccgtgt cggccaccat
    15421 gcgcttcacc gcggcctcgt cgttcacgtc gacctgcaag gcgatggcgt cactgccgag
    15481 cgccttcact gcagcctggt tcttggcctc gttcctgccg gcgatggcca ccttcgcccc
    15541 ggcctgcacg aggcccttgg cgatgcccag gccgatgccg ccgttgccgc ccgtcaccag
    15601 ggcggtcttg cctttcagat caaagggatt catgccaaaa gtgtaatggg gtaagctagc
    15661 ccacagcaat cgcggccatg gacaaattgc gaatcaacgc cgaccggctc tggggctcgc
    15721 tcatggagct ggcgcaaatc ggcgccactc ccaagggcgg cgtaagacgc ctgacgctca
    15781 ccgaggaaga ccggcgcgcc cgcgacctgt tcgcgcgccg ggcgcgcgag gccggcctcg
    15841 cggtcgacgt cgacgccatt ggcaacatct tcgcgcgccg cgcaggcgcc gatccgtcgc
    15901 tgccgccggt cgccatgggc agccacctcg actcgcagcc ctcgggcggc aaattcgacg
    15961 gcgcctacgg cgtcatggcc gggctcgagg tgctgcgcac gctgaacgac gccggcgtcg
    16021 ccacgcgcgc gccgatggaa gtggtgtcct ggaccaatga ggagggctcg cgcttccagc
    16081 ccgtgatgat gggctctggt gtgttcgcga acgtcttttc cctcgagcac gtgcgcgcgc
    16141 agaaggacct cgacggcgtc gcggtcggcg acgcgctgca agccatcggc tacgccggcg
    16201 ccgcccggcc gcaccgcctt ggcgcctatt tcgaggcgca catcgagcag ggcccggtgc
    16261 tggagaacga gaacaagacc atcggcgtgg tgcagggcgc gctcggccag cgctggtacg
    16321 acgtcaccgt gaccggccag gacgcgcacg ccggcccgac gccgatggag ctgcggcgcg
    16381 acgcgctgat cggcgccagc cgccttgtgc tggaagtgaa ccgcatcgcc cgcaccttcc
    16441 cggactacgc gcgcggcacc gtcggcttca tgcaggtgca gccgaactcg cgcaacgtca
    16501 tccccggcga ggtgcgcacg tcggtggatt tccgcaacgc gaaagctgcg acgctcgacg
    16561 cgatgtgcga ggaactcaag cgcgcggcgc gggtgatcga aagcgaatgc cgggtgctga
    16621 tcgagctgcg cgagacggtg tacttcaggc cctgcgagtt cgacgccggc ctggtggccg
    16681 gcgtgcgcga ggcggccagc gcgctcgggt acagccatcg cgacatcgtc agcggcgccg
    16741 ggcacgacgc cgtgtacgtc gcgcgcgtcg cgcccacggc gatgattttc attccctgcg
    16801 aggggggcat cagccacaat gagattgaaa gcgccgcgcc ggcggatctg accgcgggcg
    16861 gcaacgtgct cttgcacgcg gcgctgcgta aggcgggaat cgcgggcgcg gcccgcgccg
    16921 cagcctagga gaaccgacat gaacgatcgc gccacccttc ccagcctcga aaactccgcc
    16981 ttcttcatgc ctttcagcat gaaccggcac ttcaagaagg cgccgcgcct gctggcgcgc
    17041 gccgaaggcg tgtactacta caccccggag ggcaggaagc tgatcgacgg ctcctcgggc
    17101 ctgtggtgcg taaacgccgg gcactgccgc gagaagatcg tcaaggcggt gcagaagcag
    17161 gtcgccgagc tcgatttcgc gccgccgttc aacatgggcc acccgctcgc cttcgagttc
    17221 gccgacaagg tggcgagcct cgcgcccgcg gacctcaagc gcgtgttctt caccaattcg
    17281 ggctcggaat cggtcgacac ggcgctcaag ctcgcgctcg cgtaccaccg tgtgcgcggc
    17341 gagggccacc gcgtgcgcct catcggtcgc gagcgcggct accacggcgt gaatttcggc
    17401 ggcacggcgg tcggcggcat ggtcgcaaac cgcaagatct tcggcgcgct ggtggcgggg
    17461 gtcgatcaca tccgcagtac ccacgacttg gaccggaacg cctactcgcg cggtcagccc
    17521 aagcacggcg ccgagttcgc cgacgaactg gagcgcctgt gcgcgctgca cgacccctcg
    17581 accatcgccg ccgtgatcgt cgagcccgtg gcctgctcgg gcggcgtgct ggtgccgccg
    17641 gtgggctacc tcgagcgcct gcgcgcgatc tgcgacaagc acggcatcct gctcattttc
    17701 gacgaggtga tcaccggctg ggggcgcctg ggcaagacct tcggcacgca gcacttcaac
    17761 gtcatcccgg acatgatcac caccgccaag ggcatcacca acggcaccgt gccgatgggc
    17821 gcggtgctga tgcgcgagcg gctgtaccag gcgttcatgg tcgggcccga gaaggcgatc
    17881 gaggtgccgc acggctacac gtattcctcg caccccctcg cttgcgctgc ggggctcgcg
    17941 accatgcagg tgtacgaaga ggaggggctg ttcacgcgcg ccgccgagct ctggccctac
    18001 tgggaggagc aggcgcacgc gctgaagggc ctgcctaacg tcatcgacat ccgcaacatc
    18061 ggcttgatcg ccgccatcga gcttacgccg cgcaaggatg caccaggtgc gcgcgccctc
    18121 gatgcgcaca tcgcggcctt cgagcgcggc gcgtacatgc gcgtggcggc cgacaccatc
    18181 gcgctgtcgc cgccgctcat catcacgaag agcgagatcg accagctgtt cgagatcgtg
    18241 cgcggcgttc tcaagtcgac cgactgagcc gcggataagg gaggttacat gttcggcttt
    18301 ggcaagaaca aggacgaggc gcccgcttct gaagcgccgg acgatcgcgc ggcaggcggg
    18361 ggactgtcgt ccaaccgcac gcggatcgcc ctgctcgccc tggtgctggt cgccgggctg
    18421 ggctacctcg cctacgagta cctgccaggg ctgctcgacc cggcgccgcc gcccatgccg
    18481 gtgcaggcca agccgaagcc gaagcctgca cccgtggcgg tcgcccagac gaccgcagtc
    18541 ccggtcccgg ttgctgccgc ggtgaccaag ccccaggccg agccggcacc cgaacccaag
    18601 gccaagcccg caccgacgcc ggaacctgcg gcaaagcccg agcccaagcc cgtcccaaag
    18661 ccggccgcca aaccggctcc gaagcccgag cccattgcca agcccgagtc caagcccgcg
    18721 cccgtcgtcg ttgcggcggc gcccgcagcc gagccgaagc ctgcggccga gccgaagccc
    18781 gcagccgagc cgaagcctgc ggccgagccc acgcctgcac ccgccttggt ggcgcccgcg
    18841 gccgccgtcg cagccgcggt cgcgcccctg cgcgggccgc cgcccatcgt gctcaagtac
    18901 aacgacgtga tgaccgcggt gctgtacagc gatcgcgagg cggtcgcgca gctgctcgat
    18961 gtggggcgtt gggtggacaa gccggactcg aacggcctca cgcccctaat ggcggcggta
    19021 ctcgggcgcg atcccgacat ggtgcggctg ctgctcgagc gcggcgccga cccgaacctg
    19081 caggcgccgg ggggcgaggt cgcgctggag atggcgcgcg ccaacggcga cggcgcgagc
    19141 gagtcgctgc tgctgaaagc gggcgcgagg tactagctta cttggcggcg gcgcgcacga
    19201 cgccgagacg gcggccgcgc gtctgctgct tcaaccgcgg cggcgtcacc aggtcgcgca
    19261 cgaagttggg cagcgcgaac acgccctcat gcgtttcccc gttgtagtag cgcagctcgg
    19321 tcatcttgcg ctgctcgatg cgccggtcga tctcgtcggc cgtgtaggcc ttcgggtcga
    19381 gcttgtccga gcacaccgcc atcgaccata gcgacccgta cagcgggatg tacatgatgt
    19441 agggccgcac gatgcggtag atgccgttca ggcgctgggc gagctcgccc acgcgctcgg
    19501 gctgggcgac gggcgatccc aggtgcagcg tgagcgcgcc ccccggggcg agcgcctgcc
    19561 ggcactgctg gaaaaactcc gccgagtaca gcgcctcggc cggccccatc gggtcgttca
    19621 gatcgagcgc gatcaggtcg aagcgctcct gcgtctcgcg cacgaacttc atgccgtcgc
    19681 cgaccagcac gcgcagcttc gggttgtcga agacgccgtt gtgcacggcc ctgaagtgct
    19741 ccttcgccac ccggatcacg tcggggtcga tctcgcacat cgtcacctgc tcgaccgatg
    19801 gatgcttgag cgcctcctcc gaggatccgc cgtcaccgcc gccgatgatc agcaccttct
    19861 tcggcgccgg gtgcgccgtg agcgccgggt ggatcaggtt ctcgtggtag acgaactcct
    19921 ccttctcgga ggtcatgttg tacccgtcga ggcgcaggat cttgccgtag tgcggcgtgt
    19981 cgaagatctg caggcgctgg aagcgcgtct gccactcgcc gacctggcgc gcggccttga
    20041 cgtaaaagcc ggtcgagtcg ttgagcggct ccatcaccag gtgcccgccg cgctccactt
    20101 cgtggcgcgc gcactcggcg ggctggaaat gggcgacgat ggcgtcgaac agctttcgcg
    20161 ccttggcgct gttgtcgtcg ctgtagttgc agacgtagac gtcgagcgtc agcccctgct
    20221 tttcgggcca ggtgtggatg gcgaggtgcg attcggcgag caccacggtg ccggtgaagc
    20281 cggagccgtc gaactggtga aagctcgcgt ccatcgtcgt caggccggcg tcgcgcacga
    20341 attccaggca cttgtccttg aatccggcgc cgtcgagcag cagctgcggg tcgcagcggc
    20401 agccgctcag atcaccgatg agatgcagac cgttcatgtc cgttcccccc gaaaaacgag
    20461 aaggcgcagc cctgcaccaa agaatccagg ctgcgccccc gcgttaccgc cttgcggcga
    20521 aaaaccgagt tgctctaggt cgtccaattc gtgcaacacc ccgcttgggc cgccccgcac
    20581 ttacgcatgc gaggctgagc gccgatttaa atttggcgcc ggatctccgc tgaaaccgcg
    20641 tcgatcaggc gccgcgcgat cgagattttg cttggaagtt taggcaattg caatacgtcg
    20701 aaccagctcg cggcctcgat ttcgccttcc tgcggccgga tctcgccgcc ggcccattcg
    20761 catacgaacg cgatcatcag gctgtgggga aacggccacg gttggctggc gaagtacgac
    20821 atgtcgccga gcgtcacgcc gacctcctcg gcgacctcgc gcgccacgca ctgctcgagc
    20881 gactcgccgg gctcgacgaa gccggcgagc gcgctgtaca tgccggccgg gaagtgcggg
    20941 ctgcgcccga gcagcagctc gcgcccgcgc ctcacgagcg ccatcaccgc gggcgccacg
    21001 cgcgggtacg cagacaggcc gcaggccggg cagcgccgca cgcgctcttc ggcctgcggc
    21061 tcggtgcgcg tgccgcagcg cccgcagaac tggtggtccc ggtcccactg cagcagctgc
    21121 agcgcgcggc cggcgagcgc gaagtgcgcg tcctcgagga ccgagaacag cgcacgcagc
    21181 ccgacccagt cgtggccggg cggcaactgc gcgtcggcga cctccgcggc ccagcagtcg
    21241 agcccctcta gaccccccag atatagcgtt cgaagcggag gcgaccacaa gggagtgttg
    21301 tttttccgca acggcagtcg cgcccagctc ggccgctcgc gcacccgcgg atcgtcgccc
    21361 gggcgcgggt cgggcgggcc gacctcgacc agcagccggt cgccgcggaa tacgaaccac
    21421 aagccctcgg catcgccggc gccgtgcacg cggtgctcga acgcccgcgg gctggagaag
    21481 gggaggagca gcatgtcgcc gagtataatt gcgcgctccg atgaatcccc cagcgtccga
    21541 tcgcgagctc gctgacgcca tccgcgcgct cgccatggat gcggtcgagg ccgcgaaatc
    21601 gggccatccc ggcatgccga tgggcatggc ggagatcgcg gtggcgctct ggcagcgcca
    21661 cctgcgccac aacccggcca atccggcgtg ggccaaccgc gaccgcttcg tgctctccaa
    21721 cggccatggc tcgatgctgc tctatgcgct gctgcatctc accggctacg acctgccgat
    21781 ggcggagctc aggcgcttcc gccagctcgg ctcgaagacg ccggggcacc ccgagctcgg
    21841 ctgcgcgccg ggcgtcgaga cgaccaccgg gcccctgggg cagggcgtca gcaacgccgt
    21901 cggcatggcg atcgccgagc gcttgctggc cgccgaattc aaccgcccgg ggttcgacgt
    21961 catcgaccac tacacctacg tgttcctcgg cgacggctgc atgatggaag gggtctcgca
    22021 cgaggcttgc tcgctcgccg gcacgctcgg cctgggcaag ctgatcgccg tgtacgacga
    22081 caacggcatc tcgatcgact ccgacaaggg ccagatccgc cagtggtaca ccgacgacgt
    22141 ccccaagcga ttcgcggcgt acggctggca ggtgatcgcg aacgtcgacg ggcacgacgt
    22201 cgaggcgatg gacaaggcgc tgaagaaggc gaagcgtgaa aaatcgcgcc cgagcctgat
    22261 ctgcgcgaag acggtgatcg ccaagggcgc gccgaagaag gccaataccg gcgcggcgca
    22321 cggcgcgccg ctcggcgcgg aggaagtggc ggcgacgcgc caggcgctgg gctggccgca
    22381 cgcggcgttc gagatcccgc aggcgatcta cgacggctgg gatgcgcgcg cggccggcaa
    22441 gcgcgccgag cgccgttgga ccaagctcgt tgccgcctac gaaaagcagt ttccggccga
    22501 ggcggcggag ctgcgccggc gcaccgcggg caccctgccc gccgattttg cggcgcgcat
    22561 cgcggcgctg accgccgagc tgcatgccaa ggcggaaagc gtggccacgc gcaaggcctc
    22621 gcaggcggtg ctggaagcgc tggtgccggc gctgcccgag ctcgtcggcg gctcggccga
    22681 cctgacgggc tcgaacctca cgatggtgaa ggcctcgcgc gtcgtcggcc gcgagggcgg
    22741 cggcaactac ctgttctacg gcgtgcgcga gttcggcatg tgcgcggtga tgaacggcct
    22801 tgcgctgcac ggcggcgtgc tgccctacgg gggcaccttc ctcgtgtttt ccgactacgc
    22861 gcgcaacgcc ctgcggctcg ccgcgctcat gcgccagcgc gtgatctacg tcttctcgca
    22921 cgattcgatc ggactgggcg aagacggtcc cacgcaccag cccgtggaac acgcggccag
    22981 cctgcgactc ataccctaca tggacgtgtg gcggccctgc gatgcggcgg agacggcgat
    23041 ggcctgggca gtggcgctcg agcgccgcga cggcccctcg gcgctcctgt tgtcgcggca
    23101 gaacgtggcg cccgcgccgc gcgccgccga ggcgctcgag ctcgcgcgcc gcggcggcta
    23161 cgtgctgtcg gatgcgccct gggccaaggc ggtgctgatc gccaccggcg cggaagtgcc
    23221 gatcgcgctg gaggcgcaga aacgcctggc ggcggcagac ctgccggtgc gcgtggtgtc
    23281 catgccctcg accacggtgt tcgaccgcca ggacgcgggc taccgcgaag gcgtgctgcc
    23341 gcccgcgctg ccgcgcgtcg ccgtcgaggc gggcgtcacg gatttctggc gcaagtacgt
    23401 cggcctgcag ggcgccgtgg tcggcctcga ccgcttcggc gaatcggcgc ccgcggggga
    23461 tctgttcaag cacttcggct tcacggcaga gaacgtcgaa aaggcggtaa ggagcgtcat
    23521 cccatgacca tcaaagtagc gatcaacggc ttcggccgca tcggccgcaa catcctgcgc
    23581 gcgcactacg agggcaagaa gaagcacgcg ctgcagttcg tcgccatcaa cgacctgggc
    23641 gatccggcga ccaacgccca cctgctgaag tacgacaccg cgcacggccg gtttcccggc
    23701 agggtcgcgg tcgagggcga cgccatcgcg gtcgagggcg acaagctcaa ggtgctcgcc
    23761 gagcgcgacc cggccaagct gccctggaag gcgctcggcg tcgacgtggt gctggaatgc
    23821 acgggcctgt tcaccggcaa ggcgaagggc ggcgcggcgc atgtttccgc cggggcgaag
    23881 aaagtaatca tctcggcacc gggcgacaag gacgtggacc gcaccatcgt catgggcgtc
    23941 aaccacgcct cgctcaaggc gtccgacacg gtgatctcca acgcctcctg caccaccaac
    24001 tgcctcgcgc cgctggtgaa ggcgctggat gaccgcatcg gcgtcgtctc gggcctgatg
    24061 acgacggtgc actcgttcac caacgaccag gtgctgaccg acgtctacca caaggacctg
    24121 cgccgcgcgc gctcggcgac ccacaacatg atcccgacga agaccggcgc cgcggccgcc
    24181 gtggggctgg tgctgccgca cctgaacggc aagctcgacg gctacgccat ccgcgtgccg
    24241 acgatcaacg tctcgatcgt cgacctcacg ttcgtcgcga agaaggcgac cacggtcgac
    24301 gcggtgaacc aggcactgca gcaggccgcg cagaccgacc tgaagggcat cctcgagtac
    24361 acgagcgagc cgctcgtgtc ctgcgacttc aaccaccacc cggcgagctc gatcgtcgat
    24421 ggcgcgctca ccaaggtggc cgacggcacg ctggtgaagg tcgccagctg gtacgacaac
    24481 gagtggggat tttccaaccg catgctggac gccgccgtcg cgctgatgag cgcgaagtag
    24541 cgccgatgcg cttcaagcgc atggcggacc tggacctgcg cggcaagcgc gtgttcatcc
    24601 gcgccgacct gaacgtgccg ctggaggccg gccgcatcac cgacgacacg cgcatccgcg
    24661 cctcggtccc gggcatccgg ctcgcgctcg agaagggcgc ggcggtcatg gtcacatcgc
    24721 acctcgggcg tcccaaggaa ggcgagctca agcccgaaga ttcgcttgcg ccggtggccc
    24781 agcgcctcgc cgaactgctc ggacgcgagg tgccgctcaa gcgcgactgg gtcgagggcg
    24841 tggaggtgaa gccgggcgag gtggcgctgc tcgagaactg ccgcgtcaac atgggcgaga
    24901 agaaggacga cgaggcgctg gcgaagaaaa tcgccgcgct gtgcgacgtc tatgtgaacg
    24961 acgccttcgg caccgcgcac cgcgccgagg ccaccaccca cggcgccgcc cgcttcgcca
    25021 aggtcgcgtg cgccgggccg ctgatggccg cggagctgga cgcgctcggg aaagcgctcg
    25081 cgcacccgga gcggccgctc gtcgccatcg tcgccggctc caaggtctcg accaagctca
    25141 cggtgctcgg cgaacgtgcg aagaaagtcg atcagctgat cgtcggcggc ggcatcgcca
    25201 acacgttcat tctcgcctcg gggctgccgg tgggcaagtc gctcgtggag aaggatctgg
    25261 tggacgaggc ccggcgcatc atgcaggcga cgcgggtgcc gctgcccgag gacgtggtgg
    25321 tcggcaggag cctcgaggac acgcgcggcg tcaccaaggc ggcgcgcgac gtgggcgcgg
    25381 acgagatgat tctcgatatc ggtccgaaga cggcggcggc ctacgcgggc acgctcgcgg
    25441 gcgcgggcac catcctgtgg aacgggccgg tcggggtgtt cgagaacaac gccttctcgc
    25501 gcggcacgga gacgctggcg cgcgcggtcg ctgcgtccgg cgcgttctcc atcgccggcg
    25561 gcggcgacac cgttgccgcg atcaacaagt tcgggatggc ggagggcgtg agctacatct
    25621 cgaccgccgg cggcgcgttc ctcgagttcc tggagggcaa gaccctgccg gcggtggccg
    25681 cgctcgaggc gcgcgcctaa gccgccgcag cgggcggtgc tacactgcgc gcgatgctcc
    25741 gcagcaccaa gatcgtcgcc acgctcggtc ccgcctcgag cgacccggcg gtgctcgagc
    25801 gcatgctgcg cgccggcgtg gacgtggtgc gcctgaactt ctcccacggc acggccgacg
    25861 accatcgcaa gcgcgcggag ctggtgcgcg acatcggccg caagatcgag cgcacggtgg
    25921 ggatcatgtg cgacctgcag ggccccaaga tccgcatcgg caagttcagg gacggcccgg
    25981 tgacgctcga gacgggcgcg cgctttgtgc tcgacgccgc gtgcgcggag ggcaatgccg
    26041 agcgcgtggg tcttgactac aaggagctgc cgcgcgacgt cgaggagggc gcggtgctgc
    26101 tcctggacga cggcaagatc gtcctcgagg tgaccgcggt gcgcggcgac gaggtgcaga
    26161 cgcgggtgcg ccacggcggg gtgctgtcca acaacaaggg catcaaccgc cagggcggcg
    26221 gcctgaccgc tcccgcgctg accccgaagg acatggagga catccgcacg gcagcgcagc
    26281 tgcacgccga tttcctcgcg gtgtcgtttc cgaagtccgg cgcggacatg tacatggcgc
    26341 gggagctgat gcgcgcggcc ggcggcaagg cgatgctgat cgccaagatc gagcgcgccg
    26401 aggcggtgaa gcacgaggcc ctgctcagca tcatggccgc ctgcgacggc atcatggtgg
    26461 cgcgcggcga cctggccgtc gaggtgggcg acgcggccgt gcccgcactg caaaaacgca
    26521 tgatccggct cgcccgcgag cacaacaagc tcaccatcac cgccacgcag atgatggaat
    26581 ccatgatctc gagcccggtg cccacgcgcg ccgaggtctc ggacgtggca aacgccgtcc
    26641 tcgacggcac cgatgcggtg atgctctcgg cagagtcggc gagcggcaag tacccggtac
    26701 agaccatcga ggcgatggat cgcatctgcg tggaggccga gcagacgcag tcgatcagcc
    26761 tcgaccagga cttcctcaac cgggtgttca cgcgggtgga ccagtcgatc gcgatgggcg
    26821 ccctgttcac cgcgttccac ctcaaggtga aggccatcgc cgcgctgacc gaatcggggg
    26881 ccacggcgct ctggatgagc cgcctgaact gcggcgtgcc gatctacgcg ctcacctccc
    26941 agacctcgac gcgctaccgg tgctcgctcc ttcgcgatac ctatccgctg atggtgaagt
    27001 acgtcggcca cgatcgcgag gagctgctgc gcgaggccga gaaggtgctg gtcgacaacg
    27061 gcgtggtgaa gaacggcgac ctgatcgtgc tcaccatcgg cgagccgatc ggcaaggcgg
    27121 gcggcaccaa cacgatgaag atcgtgcggg tgggcgagca cctgcgggca taataggcgc
    27181 ggaacggagg atcccatgac cgccatcacg ccgaaagtcg ccgcccacgc gacccttgcg
    27241 ccgcttgctt cgcgcttcgt cgaggtggcg aagctgcctt gggaagacac ccgctttccg
    27301 ggcgtgcggc agaaggtgct ggtcgtcgac cgcgacagcg gcgtggcgac cgtgctcatc
    27361 catatggcgc ccggcgccaa gcttccggac cacgagcacg tgctgctgga gcagacctac
    27421 gtgctggagg ggagcctcgt gtgcggagaa ggcgagtgca aggcgggcga cttcgtgtgg
    27481 cgcccggccg gcagccggca ctcggcctgg ggcgggccgc agggcggcac gatgctcgcg
    27541 atcttccaga tcgccaaccg cttcttcgat cccgacggac gcgagaccga cgtgaacggc
    27601 caggactggg aagcggcctg gggcaagacg ctgtttcgcg aggcgtaaaa aaaagcgggc
    27661 gcttccgcgc ccgctttcgg gtctgctgcg aaacgcgcta cttgccgatg atttcgccga
    27721 agcgtaccca gccctcaccg tcgaagcgtg ccagctgctc cgattcgatc ggcgcgaagt
    27781 ccgtcggcga cgtattgatg cgaatacccg ggagcaggac cgtcggccgg aaatccttga
    27841 gattggccgc ctgcttcatg atgttggcgc ggctcaggtc gttgccggcc tgcttcagca
    27901 cctgctccat ggtctgcgcc accgcgtagc cg
//
LOCUS       DBNHKE010000014        31668 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_848749, whole genome shotgun sequence.
ACCESSION   DBNHKE010000014 DBNHKE010000000
VERSION     DBNHKE010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 31668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31668
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_848749"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="AC16O9_00845"
     CDS             complement(<1..694)
                     /locus_tag="AC16O9_00845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829007.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HIR6515197.1"
                     /translation="MLDYGPDAILMAGLDGRNWRLKDYEAREGYAALRRLLKEGVAPD
                     AVIAEVKKSALRGRGGAGFPTGLKWSFMPRQYAGAKYLVCNSDEGEPGTCKDRDLLRY
                     NPHAVIEGMIIAGYAMGATAGYNYIHGEIWAVYEQFEEALKEAYDAGYLGKDILGSGF
                     RFELYAHAGYGAYIVGEETAMLESLEGKKGQPRLKPPFPASYGLFGKPTTVNNTETLA
                     AVPWIMRHGANAF"
     gene            complement(696..1178)
                     /gene="nuoE"
                     /locus_tag="AC16O9_00850"
     CDS             complement(696..1178)
                     /gene="nuoE"
                     /locus_tag="AC16O9_00850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR6515198.1"
                     /translation="MLSAESLKKIDREVAKYPPDRKQSAVMAALIVAQDEKGWLSTEV
                     MDFVAQYLGMAPVAVHEVASFYTMYNRQPTGTHKLTICTCLPCGLQGALAAADHLRKR
                     LGIDFNETTPDGWFTLKEGECMGACAMAPVVLVDNKRMCDYMTNEKLDALVDELSRKS
                     "
     gene            complement(1184..2437)
                     /locus_tag="AC16O9_00855"
     CDS             complement(1184..2437)
                     /locus_tag="AC16O9_00855"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR6515199.1"
                     /translation="MAEIRNYTVNFGPQHPAAHGVLRLVLELDGEVVQRADPHIGLLH
                     RATEKLAESRMYIQSLPYMDRLDYISMMCSEHAFCMSLEKLLGVEVPVRAQYIRVMFD
                     EITRIMNHLFGVATLGLDLGAMTVFLYGFREREDLLDCYEAVSGARMHAAYYRPGGVY
                     RDLPDSMPKYEAQQSRNARALAEMNANRQGSLLDFIEDFTRRFPKCVDDYETLLTDNR
                     IWKQRTVGIGVVSPEQALARGFTGPMLRGSGVEWDLRKKQPYEVYDRLDFDIVVGRNG
                     DTYDRYLVRMEEMRQSNRIIKQCVDWLRKNPGPVITDNHKVAPPSRAEMKGSMEELIH
                     HFKLFTEGMHVPEGEAYAGVEGPKGEFGVYFISDGANKPYRMKLRSPAFTHLSAMDEM
                     ARGHMLADAVAIISTLDIVFGEIDR"
     gene            complement(2437..3027)
                     /locus_tag="AC16O9_00860"
     CDS             complement(2437..3027)
                     /locus_tag="AC16O9_00860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR6515200.1"
                     /translation="MTPKLQRLFDALTAAGFAPVERLGELTVEVPPEGYLDACRRLRD
                     EPGLKFEQLIDLCGVDYSTYGNQPWEKGRFAAVLHLTSVTHNWRLRLRAFCADDALPM
                     LDSLVEVWPGVNWFEREAFDLFGIVFEGHPDLRRILTDYGFVGHPFRKDFPISGHVEM
                     RYDPEQRRVVYEPVTIEPREITPRVVREPNYGEERH"
     gene            complement(3024..3500)
                     /locus_tag="AC16O9_00865"
     CDS             complement(3024..3500)
                     /locus_tag="AC16O9_00865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIR6515201.1"
                     /translation="MGIEGVMPQGFVTAKLDTVINWTRTGSLWPVTFGLACCAIEMMH
                     AGASRYDLDRFGIVFRPSPRQADVMIIAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGADRIVPVDIYVPGCPPTAEALLYGILQLQNKIRRTNTIAR"
     gene            complement(3504..3860)
                     /locus_tag="AC16O9_00870"
     CDS             complement(3504..3860)
                     /locus_tag="AC16O9_00870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR6515202.1"
                     /translation="MLAEYFPILLFILVGLAVGVGPMVLGKLLGPSRPDPEKLSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVALNDIGLAGFVAMMLFLGIL
                     VVGFVYEWMKGALEWE"
     gene            complement(3864..3951)
                     /locus_tag="AC16O9_00875"
     tRNA            complement(3864..3951)
                     /locus_tag="AC16O9_00875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3915..3917),aa:Leu,seq:gag)
     gene            complement(3983..4363)
                     /gene="secG"
                     /locus_tag="AC16O9_00880"
     CDS             complement(3983..4363)
                     /gene="secG"
                     /locus_tag="AC16O9_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR6515203.1"
                     /translation="MGQWITLIIVAHVLIALAIIGLVLLQHGKGADMGSGFGGGASSS
                     LFGATGSANFLSRATAALATIFFLTSLGLAYLASEKPRTEAGTVMDAVKPPPAPPAGG
                     EIPRPQALPPSGGDAGSPASGIPK"
     gene            complement(4368..5090)
                     /gene="tpiA"
                     /locus_tag="AC16O9_00885"
     CDS             complement(4368..5090)
                     /gene="tpiA"
                     /locus_tag="AC16O9_00885"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR6515204.1"
                     /translation="MRARLVAGNWKMHGSLAANRALLDAIVAGVQGVECAVCVPFPYL
                     AQAAERLRGTRIAWGAQNLSEHASGAYTGEVSAAMLGEFGCRYVLVGHSERRQLYGES
                     DAAVAAKFAAARSGGITPILCVGETLPEREAGRTQDVVARQLDAVLARGEIGDAVLAY
                     EPVWAIGTGRTASPDQAQEVHGLLRKRLPATTRILYGGSVKADNAAALFAMPDVDGGL
                     IGGASLKAQDFIAICKEAEQKG"
     gene            complement(5120..5650)
                     /locus_tag="AC16O9_00890"
     CDS             complement(5120..5650)
                     /locus_tag="AC16O9_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515205.1"
                     /translation="MLRAALPLRLALPVLEPFLADNVAKEVRKDALLIQRAGAGRSPG
                     DARELLAAAREIDRAFLARIGDAPVRIEIPYARIEPVRLRRIELGLDTAQRILEAWGQ
                     RRRARDAFAPGELERRVLEMLQLYAEETQLLSHAVRLPALLAPLRERAARHLRDAMTE
                     AARALVRGSHAAGQKG"
     gene            complement(5719..6480)
                     /locus_tag="AC16O9_00895"
     CDS             complement(5719..6480)
                     /locus_tag="AC16O9_00895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6515206.1"
                     /translation="MNPPPVATREIAREERFNVVWTQELNDVFADVAPYYDRANYIAS
                     LGLWGWFLRQFMATVDIRPGERVLDVCAGTNAIGIALLKREPTLEVHAIDRSAAMQEV
                     GRQRAQAAGFRIQSVIDDVHTLPYPDNSFDVATLQFASRHLRIRRVAAEIRRVLKPGG
                     RFYHCDMLRPGNRTVERLYYAYLRFCLWFTGFVFRSGPAALNCKEYFIQALQMFYSAR
                     ELTDMLEDVGYQDVTSRTVFSGMLGFHRAVKPRAA"
     gene            complement(6495..6836)
                     /gene="frdD"
                     /locus_tag="AC16O9_00900"
     CDS             complement(6495..6836)
                     /gene="frdD"
                     /locus_tag="AC16O9_00900"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HIR6515207.1"
                     /translation="MAVSNKPIVWGPFAAGGTLTAFLTPVLVLLTLLAALGHAPDLLA
                     YDRLHAFAGHWLVKLALAGTVFLSLWSAAHRLRITCYDLGIRADTWVAAIVYAGALAG
                     SGAAVLYLARI"
     gene            complement(6839..7240)
                     /locus_tag="AC16O9_00905"
     CDS             complement(6839..7240)
                     /locus_tag="AC16O9_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HIR6515208.1"
                     /translation="MAEEVKRKPYVRAVTSEWIFRHPRYIRYMVREFSCLFIGAWTLL
                     MVWGLKQLAQGPQAWAAFLELLQHPVSIAFHLAALAFAVYHSVTWFNLTPKALPVQRG
                     EAFVPDGAISGAHFAVWAVLSLAVLFLAGAF"
     gene            complement(7225..7968)
                     /locus_tag="AC16O9_00910"
     CDS             complement(7225..7968)
                     /locus_tag="AC16O9_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR6515209.1"
                     /translation="MSTRALELEVLRYNPESDREPRFQRYAIECPEEWVVLDALNHVK
                     ESVDPTLSYRWSCHMAVCGSCGMMINGEPKLACKAFLRDYAGVIRVEPLAHFPVERDL
                     VTVIDDFMAKLARVRPYLMPKHAKAIEAGEYRQTPAELARFKRYSMCINCMLCYAACP
                     EYALIPKYVGPAAIALAHRWNQDSRDGGRDARQQEIAATEGAWECSFVGACSEVCPEH
                     VDPAGAIQQVKIRSTVDWYTQHLMPWLKK"
     gene            complement(7965..9764)
                     /gene="frdA"
                     /locus_tag="AC16O9_00915"
     CDS             complement(7965..9764)
                     /gene="frdA"
                     /locus_tag="AC16O9_00915"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HIR6515210.1"
                     /translation="MRERRKGRMPAVEIIRTDVAIIGAGGAGLRAAIALAQEAPSLRI
                     ALISKVYPMRSHTCAAEGGAAGVIKPDDSLEHHFNDTVGGGDWLSDQDAVDYFIREAP
                     GELIQLEHWGCPWSREADGAVAVRPFGGMKKQRTWFAADKSGFHILHTLFQTSLQFPS
                     IQRYDEFYVTELLVDDGRCAGLTALEMRSGQMRCFQARAVIIAGGGAGRMFPFTTNGA
                     IKTGDGMALAYRAGVPLKDMEFVQYHPTGLPSTGTLLTEACRGEGGVLVNKDGRRFLQ
                     DYGMGPETPLGKPVLKTMELGPRDRVSQAFWQEQKKGRMVPTQWGECMLLDMRHLGEK
                     KINERLPLVRDMSISYMGVDPVTDPVPVRPVVHYMMGGIHTDINAATPLAGLFAAGEC
                     ACVSINGANRLGSNSLTELLVFGRRAAQSAMQYVGVGAGARPAGDAALAALAAAAQGR
                     VRELVQRTGRRDTVAGLRKEMMDTMEQHAGIYRSGEGLEAACAKLADLRRRYGGIELH
                     DKTNVYNTDLLQALELGSMLDCAETVVQSARARKESRGAHQRLDFTARDDEKYLRHSL
                     AYYQASAAPRVDYCDVVITRSQPGVRDYSGGHE"
     gene            complement(9803..10597)
                     /gene="pstB"
                     /locus_tag="AC16O9_00920"
     CDS             complement(9803..10597)
                     /gene="pstB"
                     /locus_tag="AC16O9_00920"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR6515211.1"
                     /translation="MVAVPSQLAAAAQAKAAVKDLNFYYGSFQALKTLSMPVYEKQVT
                     ALIGPSGCGKSTFLRCFNRMHDLYPGNRYEGEIRLYPDDVNILAHAVDPIEVRMRISM
                     VFQKPNPFPKSIYENVAYGLRVRGEGRRVTLNEKVEEALRNAALWDEVKDRLQEPAFN
                     LSGGQQQRLCIARALATDPEILLFDEPTSALDPIATASIEELIHDLKNKVTILIVTHN
                     MQQAARVSDYTAYMYLGELIEYGVTDEIFIKPRKKQTEDYITGRFG"
     gene            complement(10591..11517)
                     /gene="pstA"
                     /locus_tag="AC16O9_00925"
     CDS             complement(10591..11517)
                     /gene="pstA"
                     /locus_tag="AC16O9_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR6515212.1"
                     /translation="MPHARRLEEIRRLIAAHKRWDAIFGVVGVLALMLGVLTFAALFI
                     DMALDGVPRLTGDFFTNFPSRRAGQAGILSAWVGSTLVMLVTAFVAVPLGVAAGVYLE
                     EYAAKHWVTDVIEINITNLAGVPSIVYGLLALGLFVYQFGFGQSILSAGLTLALLILP
                     VVIVATREAIRSIPQGIREGAYALGATQWQVCKDHIIPYSMPGILTGVIIGMARAIGE
                     TAPIITIGALTFIAFLPPSPVQAEPPFVSFDWVMSPFTVMPIQMFNWTSRPEAAFHQN
                     AAAAGFVLVLMTLGMNAIAIWLRYRLRKRIKW"
     gene            complement(11517..12434)
                     /gene="pstC"
                     /locus_tag="AC16O9_00930"
     CDS             complement(11517..12434)
                     /gene="pstC"
                     /locus_tag="AC16O9_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR6515213.1"
                     /translation="MVAPDVRAGLERRRARIARERVIEGALFLAALVSVFTTVGIIYV
                     LVKESVVFFSHVPLWDFLTDTQWTPLFDDAHFGIMVLLSGTLTSSMVALGIAVPVGTV
                     IAIYLSEFAPFGVREVAKPFLEMLGGVPTIVYGYFALLFVTPLLQTIYPELPGFNLLG
                     AGIVMGIMIIPYVSSIAEDAMRAVPMSLREGSYAMGATRFQTATRVVVPAAISGIASA
                     YILGISRAVGETMILAVAAGMQPNLTFNPLEPAATITAFIVQVALGDLPHGSVGYQTI
                     FAAGLTLFLVTLAFNIGGHVLRKRFREAY"
     gene            complement(12446..13423)
                     /locus_tag="AC16O9_00935"
     CDS             complement(12446..13423)
                     /locus_tag="AC16O9_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6515214.1"
                     /translation="MRTLAVAAAFLASAPVLAQPLVKIDGSSTVFPVTEAVAEDFQKS
                     KRGAIRVTVGISGTGGGFKKFCRGEIDVTGASRPILKKEMQACRKAGVEYFELPVAFD
                     AITVVVNPKNKFIQQLTIAEMKKMWEPSAQNTVTQWNHVNPAWPAEPMKLFGPGADSG
                     TFDYFTEAVVGRAKSSRGDFTASEDDNVLVQGVSRDVNGLGYFGYAYYVENKDKLKAV
                     PIVNEKGRPVGPSLEAVVKGSYSPLARPIFIYVSAKSLARPEVKEFVRYYMTHGAKLA
                     QEVKYVPLPESAYQQAWQHVTGGKKGTVFGGVAEVGVTIEELLRREAKL"
     gene            complement(13541..14566)
                     /gene="arsB"
                     /locus_tag="AC16O9_00940"
     CDS             complement(13541..14566)
                     /gene="arsB"
                     /locus_tag="AC16O9_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HIR6515215.1"
                     /translation="MSLFERWLTLWVLLCIVAGVALGHWLPAPVEALGRMEVASVNLP
                     VGVLIWVMIIPMLLRIDFAAMGEVRRHWRGIGVTLAVNWLVKPFSMALLGWFFLRHVF
                     APWLPAEQIDSYVAGLILLAAAPCTAMVFVWSRLTQGDPYFTLSQVALNDAIMVVAFA
                     PIVALLLGLSAIVVPWDTLLTSVVLYIVVPVILAQLWRASLLKRGEARLQATLARIQP
                     WSVAALLATIVLLFAFQGRQILAQPLIIAILAVPILVQVYFNSGLAYLLNRRLRVAHS
                     VAGPSALIGASNFFELAVAAAITLFGFDSGAALATVVGVLVEVPVMLSVVYVVNGTRR
                     WYERVPA"
     gene            complement(14568..15002)
                     /locus_tag="AC16O9_00945"
     CDS             complement(14568..15002)
                     /locus_tag="AC16O9_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HIR6515216.1"
                     /translation="MKRLNVHVAVHDLEQSIRFYKALFDAEPTVKKDDHAKWQLEDPR
                     VNFAISKRGAKAGLDHLGIQVDDGAELGQLRSQLAQADVAVMDQKNATCCYAKSDKHW
                     TLDPQGIAWESFHTLEAAPRYGEDTRVEVKEAKAACCVPMTA"
     gene            complement(14968..15315)
                     /locus_tag="AC16O9_00950"
     CDS             complement(14968..15315)
                     /locus_tag="AC16O9_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6515217.1"
                     /translation="METKQAVQALSALAQDTRLAIFRLLVQAGPEGMSAGQIGEQLDL
                     APATLSFHLAGLTRAGLAQSRQEGRFVIYSADFTAMNALVGFLGENCCGGAACNPSTG
                     EKDEAPKRTRRRA"
     gene            complement(15356..16708)
                     /gene="glmM"
                     /locus_tag="AC16O9_00955"
     CDS             complement(15356..16708)
                     /gene="glmM"
                     /locus_tag="AC16O9_00955"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR6515218.1"
                     /translation="MTRTYFGTDGVRGTVGEAPMTADFVLRLGYAAGKVLAAREAGAG
                     DRPGVLIGKDTRISGYMIEAALEAGFSAAGVDVLLCGPLPTPGVAYLTRALRLAAGVV
                     ISASHNPYGDNGIKFFSGDGFKLPDAVESEIEELLAQPIGCNASAHLGRARRVDDAGG
                     RYVEFCKSTFPADLDLRGLRIVVDCAHGAAYSVAPHVFHELGADVVSVGVSPDGFNIN
                     DLFGATSPAQLQAAVMRHEADLGVALDGDGDRLVMADAQGALYDGDQLLYAIARHRAA
                     RGRVAGVAGTLMTNLALEQAFARLGVAFARAKVGDRYVLELMRERGWQLGGENSGHII
                     CLDRHTTGDGIVSALQVLAAMRESGKTLGELTGDLVMFPQVLVNVQVPRGFDWQKDRA
                     IAAARVEAEKSLDGKGRVLLRPSGTEPLLRVMVEGESRQTVEAAAKSIADAVRRAATG
                     "
     gene            complement(16705..17523)
                     /gene="folP"
                     /locus_tag="AC16O9_00960"
     CDS             complement(16705..17523)
                     /gene="folP"
                     /locus_tag="AC16O9_00960"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6515219.1"
                     /translation="MTLRCGRFPLPLDRALVMGVVNVTPDSFSDGGRFLDSEAAVAHA
                     CRLVADGADIIDIGGESSRPGAAPVDEREELDRILPVIERMRGVPVSVDTRRPGVMRA
                     ALAAGASMINDIEALAAPGALEAVAASDCAVCLMHKKGEPATMQDEPSYDDVVGEVGE
                     FLAGRIAACERAGIARDRIVADPGFGFGKTVEHNLTLLRRLPEFARLGVPVLAGWSRK
                     SALGRITGRDAAGRLPASLAAALLALLGGARILRVHDVKETRDVILVWDAWRKA"
     gene            complement(17520..19412)
                     /gene="ftsH"
                     /locus_tag="AC16O9_00965"
     CDS             complement(17520..19412)
                     /gene="ftsH"
                     /locus_tag="AC16O9_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6515220.1"
                     /translation="MNNMLKNLVIWLVIGLVLMTVFNQFNTRQVTQAPMEYSQFLEEV
                     KSGRISKVTIEGRQLKATTAEGKRVTSYSPGDIWLVSDLLKYGVKIEAKPEEEPSLLM
                     NIFVSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSKARMLDESTNTVTFADVAGCE
                     EAKEEVSELVDFLRDPSKFQKLGGRIPRGVLMVGSPGTGKTLLARAIAGEAKVPFFTI
                     SGSDFVEMFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFEATAGVIVIAATNRPDVLDPALMRPGRFDRQVVVPLPDIRGREEILK
                     VHMRKVPLAPDVKADIIARGTPGFSGADLANLVNEAALFAARANKRLVDMDDFERAKD
                     KIMMGAERRSMVMPEEERRNTADHESGHTVVARLLPKTDPVHKVTIIPRGRALGVTMQ
                     LPEQDRYSQDRDRLLNTITVLFGGRIAEEIFMHQMTTGASNDFERATEIARRMVTQWG
                     MSDELGTMVYGENEGEVFLGRSITTHKNVSEATLQKVDAEIRRIIDTQYKLARKLIEE
                     NRDKVEAMAKALLEWETLDADQINDVMQGRPPRPPKVTSTTTTPTGGAANDGAAGTTA
                     PASPAV"
     gene            complement(19494..20096)
                     /locus_tag="AC16O9_00970"
     CDS             complement(19494..20096)
                     /locus_tag="AC16O9_00970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HIR6515221.1"
                     /translation="MAKSSKAWLRRHVTDAYVRKAKEAGYRSRAAYKLLEIDAKERLL
                     KPGMRVLDLGAAPGGWSQVAADRVRPGGVVVAVDLLEMKPIHGVTVFKADFREADLAG
                     ALGGKADVVLSDMMPNITGVPLVDQARAAELLRAAIELCGKVLKPEGVFLAKMFHGEA
                     FDEVRAALKASFQTVAVRKPAASRGESRENYLLARGVISH"
     gene            20121..20498
                     /locus_tag="AC16O9_00975"
     CDS             20121..20498
                     /locus_tag="AC16O9_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014087.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HIR6515222.1"
                     /translation="MDRKLLKARAHALHPILQLGEKGLTDAVVAEIDRALAHHELIKV
                     RAAPLNRDAREVALASICERTGAAAVQHIGKIFVLFREKPIEAQPDDPVRPPARARAA
                     PPGRRRSEKRARRSPGRGRRRNR"
     gene            complement(20681..21154)
                     /gene="greA"
                     /locus_tag="AC16O9_00980"
     CDS             complement(20681..21154)
                     /gene="greA"
                     /locus_tag="AC16O9_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR6515223.1"
                     /translation="MAKTPMTTRGAEALRTELHRLKTVERPANSQAIAEARAHGDLSE
                     NAEYDAARNQQGFIEGRISEIEAKLGNAQIIDPKLLDADGRCVFGATVDLEEQGGEPV
                     TYQIVGEDEADIKAGRISISSPIARALIGKEAGDQVDVHTPGGIKKYEILDVRYE"
     gene            complement(21156..24398)
                     /gene="carB"
                     /locus_tag="AC16O9_00985"
     CDS             complement(21156..24398)
                     /gene="carB"
                     /locus_tag="AC16O9_00985"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR6515224.1"
                     /translation="MPKRADLHSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPITWQTVARVIEQERPDALLPTMGGQTALNCALDL
                     AREGVLEKFGVEMIGASREAIDKAEDREKFKQAMSRIGLASARSSLAHSMEEALQVQA
                     SLGFPVVIRPSFTLGGTGGGIAYNREEFVAICERGLEASPTNELLIEESVIGWKEFEM
                     EVVRDRADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASIAVLREIG
                     VDTGGSNVQFAISPEDGRLLVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNDITGGATPASFEPAIDYVVTKVPRFAFEKFPQANDRLTTQMKSVGEVMAIGRTFQ
                     ESLHKAMRGLEIGVDGLNQRTTDRETIERELGEPGPERIWYVGDAFENGFSLEEVHRL
                     TRIDPWFLAQIKEIVDLEMRLDDQKLADVDADTLRMLKRRGFSDRRLAYLFNTGEMAV
                     REKRKKLGIRPVYKRVDTCAAEFATKTAYMYSTYEEECEAEPTARRKVMVLGGGPNRI
                     GQGIEFDYCCVHAALALREAGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     DKEKPWGVIVQYGGQTPLKLARDLAAAGVPIIGTSADSIDIAEDRERFQKLLEKLKLR
                     QPPNRTARSAADAVKLAEQIGYPVVVRPSYVLGGRAMEIVHRQADLERYMKNAVKVSN
                     DAPVLLDRYLSDAIEVDVDCIADGDEVLVGGVMEHVEQAGVHSGDSACCLPPHSLAPE
                     MVQELRRQTEIMARALQVRGLMNVQYAIQGGVVYVLEVNPRASRTVPYVSKATGRPLA
                     KIAALAMAGISLAAQGIRGTITPPYYSVKEAVFPFSKFPGVDTILGPEMKSTGEVMGV
                     GQSFGEAFVKSQLAAGVRLPKGGRAFISVRDGDKSAAVAVARELVELGFTVLATRGTA
                     AALAAAGVEATPVNKVAEGRPHIVDMIKNGEVSFIVNTVEDTRTAISDSRSLRTTALQ
                     ARVTYYTTIAGAKAACAGMKYLPRLEPYRLQDLHAKLGAAPLTRTVASTS"
     gene            complement(24382..25521)
                     /gene="carA"
                     /locus_tag="AC16O9_00990"
     CDS             complement(24382..25521)
                     /gene="carA"
                     /locus_tag="AC16O9_00990"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR6515225.1"
                     /translation="MPALASAVLVLADGTVFRGRGVGAPGLAAGEVVFNTAMTGYQEI
                     LTDPSYAGQIVTLTYPHIGNTGVNPEDEESHKVFAAGLVVRDVPALMSSWRARQTLPD
                     YLAERNVVAIADIDTRRLTRILREKGAQSGCVMSGAVNDARALAAARAFPGLAGLDLA
                     KEVTVRAPYEWREGGWELGKGYRLIDNPSHHVVAYDYGIKRNILRLLAERSARVTVVP
                     AQTPAREVLRLKPDGVFLSNGPGDPEPCDYAIEAIGEILDATRIPVFGICLGHQLMGL
                     ASGAKTVKMKFGHHGANHPVKDLDTGQVVITSQNHGFAVDPASLPPSLRPTHVSLFDG
                     SLQGLARTDRPAFCFQGHPEASPGPRDIAYLFDRFAKLMADAQAR"
     gene            complement(25607..26380)
                     /gene="dapB"
                     /locus_tag="AC16O9_00995"
     CDS             complement(25607..26380)
                     /gene="dapB"
                     /locus_tag="AC16O9_00995"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR6515226.1"
                     /translation="MKVAIAGAGGRMGYALIDAVLMDSGFELAVALDAAGSPALGRAA
                     GGVKVGADLGALAGADVLIDFTRPEGTLAHLEACQKHGKAMVIGTTGFSEAQKARIAE
                     GARRLPIVLSPNFAIGVNVVFRLAQTAAAALGDAYDVEIVEAHHRHKVDAPSGTALKL
                     GELVATALGRDIRKVATHGRVGDTGERPARAIGIHAIRGGDIVGEHTVIFAGSGERVE
                     ITVRSQSRLTYAAGALRAAKWLQGRPPGLYDMFDVLGLK"
     gene            complement(26377..26712)
                     /locus_tag="AC16O9_01000"
     CDS             complement(26377..26712)
                     /locus_tag="AC16O9_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR6515227.1"
                     /translation="MLRVLLLLLALQLAGCGWLAPYRIDIQQGNFVSQEMVAQLKRGM
                     TKEQVRFVLGTPLVTDIFHASRWDYVYLLDRPGQPRVQRRLAVFFEDDKLARVEGDVV
                     AAAAAEPAR"
     gene            26734..27171
                     /gene="fur"
                     /locus_tag="AC16O9_01005"
     CDS             26734..27171
                     /gene="fur"
                     /locus_tag="AC16O9_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR6515228.1"
                     /translation="MSSAQSLKDIGLKATLPRRKVLELFENSKVRHLSAEDVYRSLMA
                     EGIDIGLATVYRVLTQFEQAGLLSRQHFETGKAVFELNQGGHHDHLVCLQCGRVEEFY
                     DAEIEQRQSEVARKRGFELRGHSLALYADCTKEDCPNRKDGRL"
     gene            complement(27168..28826)
                     /gene="recN"
                     /locus_tag="AC16O9_01010"
     CDS             complement(27168..28826)
                     /gene="recN"
                     /locus_tag="AC16O9_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6515229.1"
                     /translation="MLRVLSVRDYVIAERVELEPGPGYTVLTGETGAGKSILVDALEL
                     LVGGRADSALVREGAERAELSAEFDLDARSPLAAWLAERDLQGDPGNLILRRTLERGG
                     RSRCFINGHVATLAQLREAGEYLVDIHGQHEHQSLLRPAAQRELLDAHGGGEALARDC
                     AAAYRDWQRLRALAEDAEAQFAKHEAERAELEERRVLLDKLAPQPQEWERLAEEHRRL
                     EHGSSLLAGAQSALEALSEAEGAILPQLAAVAARLRALGEHDARLAPILELLSSAEAQ
                     AGEAMRELRHYASGVELDPQALRDAEARVEALHAAGRRFRVRAEALPELADATRTRLA
                     DLALAASPEALQKEVAAAAERYAAAAKKLTAKRKATAKALAEAVTLSMQQLAMKGGRF
                     EVRLQPLETPSAAGQESVEFEVSSHPSLPLRPLAKVASGGELSRLSLAIQLVAARVSP
                     VATLVFDEVDAGIGGAVADTVGRSLRKLGKERQVLCVTHLPQVAAQGDAQWSVLRAGA
                     RGKVATSVARLDRAARVEELARMLGGAEITATTRKHAAELLDAD"
     gene            complement(28833..29723)
                     /locus_tag="AC16O9_01015"
     CDS             complement(28833..29723)
                     /locus_tag="AC16O9_01015"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6515230.1"
                     /translation="MPKPAFSRVALIGKHTPEIADSLRALRGLLRERGCEVRVERETA
                     RRIGDGEDAASYEEIGAFADLAMVLGGDGTMLAAARSLARHKVPLAGINQGRVGFMTD
                     IALQDMRASVGAILEGRYSIEERALLDAQIMRGATRVLQTVALNEAVVGKGSQGRLIE
                     VEVRIDGEYVYALRADGLIVATPTGSTAYALSAQGPILQPGVAAFALVPLNPHVLSAR
                     PVSVADRSAIEIVLLRALDARAHFDGFALTELVQGDRLLLKRSADAIRFVHPPGYSYF
                     AMLREKLSWSEAAERGPEQD"
     gene            29741..30778
                     /gene="hrcA"
                     /locus_tag="AC16O9_01020"
     CDS             29741..30778
                     /gene="hrcA"
                     /locus_tag="AC16O9_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR6515231.1"
                     /translation="MCRMQRMLDKRAQILLKTLIERYIAEGQPVGSRTLSRHSGLDLS
                     PATIRNVMSDLEELGFIASPHTSAGRIPTPAGYRFFVDTLLVVKPLGSGEQRQLEDQL
                     HPDNPQRAIQSASQLLSQLTQFAGVVMTPRRRALAFRQIEFLRLSEKRVLLIVVTPEG
                     DVQNRVLFTERDYTPAELVSAANYINQNYAGNNFEDVRHRLQRELHEIKQDMVQLMNA
                     ALEAGNRALEEGNAPYVISGERNLLTAQDLSHDLRRLRQLFELFEHKTTLLQILDLSL
                     RGQGVQVFIGGESGVSAPDDVSVVTAPYKVDGEVVGTVGVIGPSRMAYDRVVPVVDVT
                     AKLLSSALSKP"
     gene            30784..>31668
                     /gene="hemH"
                     /locus_tag="AC16O9_01025"
     CDS             30784..>31668
                     /gene="hemH"
                     /locus_tag="AC16O9_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6515232.1"
                     /translation="MLLVNLGTPAAPTPAAVRAYLAEFLSDPRVVELPRWLWLPILHG
                     IVLRKRPAASARKYAKVWTAEGSPLAVHTARQAALLRAATGLPVEYAMRYGEPSIAAA
                     LRKLPPGPRIIPLYPQYARSTTESIRDVVSPDAEFVEQFHDHPGYIAALAALVEERPD
                     VLVMSFHGLPQRSVERGDPYQAQCLRTARLLADALRLPPDRYRVTFQSRFGPAKWLQP
                     YTSEVLAELGAARTPRVHVICPGFVSDCLETLEEIALEGRDIFLHAGGGEFKALPCLN
                     ESPRWIEALAAIATSSSRA"
BASE COUNT         4946 a        11073 c        10565 g         5084 t
ORIGIN      
        1 ggaacgcgtt tgcgccgtgg cgcatgatcc agggcacggc agcgagggtc tcggtgttat
       61 tgacggtggt cggcttgccg aacaggccgt agctcgcagg gaacggcggc ttgaggcgcg
      121 gctgcccctt cttgccctcg agcgactcga gcatcgccgt ttcctcgccg acgatgtagg
      181 caccgtagcc ggcgtgcgcg tacagctcga agcggaaccc cgagccgagg atgtccttgc
      241 cgagatagcc cgcgtcgtag gcttccttca gcgcctcctc gaattgctcg tagaccgccc
      301 agatctcgcc gtggatgtag ttgtagcccg cggtcgcgcc catcgcgtag ccggcgatga
      361 tcatgccctc gatcaccgcg tgcgggttgt agcgcagcag gtcgcggtcc ttgcaggtgc
      421 cgggctcgcc ctcgtcggaa ttgcagacca ggtacttggc gccggcatac tgccgcggca
      481 tgaacgacca tttcagcccg gtgggaaagc cggcgccgcc gcgaccgcgc agcgcggatt
      541 tcttcacctc ggcgatcacc gcgtcgggcg ccacgccctc cttgagcagg cggcgcagcg
      601 ccgcatagcc ctcgcgcgcc tcgtaatcct tgaggcgcca gttgcggccg tccaggccgg
      661 ccatcaggat cgcgtcgggg ccgtaatcga gcatgtcagg acttcctcga gagctcgtcc
      721 accagcgcgt cgagcttctc gttcgtcatg taatcgcaca tgcgcttgtt gtcgaccagc
      781 accacgggcg ccatggcgca ggcgcccatg cattcgccct ccttcagcgt gaaccagccg
      841 tccggcgtgg tctcgttgaa gtcgatgccg aggcgcttcc tgaggtggtc ggctgccgcg
      901 agcgcgccct gcagcccgca cggcaggcag gtgcatatcg tcagcttgtg cgtgccggtc
      961 ggctggcggt tgtacatggt gtagaagctc gccacctcgt ggaccgcgac cggcgccatg
     1021 ccgaggtact gcgcgacgaa gtccatgact tcggtcgaca accagccctt ttcgtcctgg
     1081 gcgacgatga gcgcggccat caccgccgat tgcttgcgat cgggcgggta cttcgcgacc
     1141 tcgcggtcga tcttcttcag cgattcggcc gagagcatgc gcgctaccgg tcgatttcgc
     1201 cgaacacgat gtccagcgtg ctgatgatcg ccaccgcgtc ggccagcatg tggccgcgcg
     1261 ccatctcgtc catcgccgaa aggtgcgtga aggccgggga gcgcagcttc atgcggtagg
     1321 gcttgttggc gccgtcggag atgaagtaca cgccgaattc ccccttcgga ccctcgaccc
     1381 cggcataggc ctcgccttcg ggcacgtgca tgccctcggt aaagagcttg aagtgatgga
     1441 tcagctcctc catgctcccc ttcatctcgg cgcgcgaggg cggcgccacc ttgtggttgt
     1501 ccgtgatcac cggccccggg ttcttgcgca gccagtccac gcactgcttg atgatgcggt
     1561 tcgactggcg catctcctcc atgcgcacca ggtagcggtc gtaggtgtcg ccgttgcggc
     1621 ccaccacgat gtcgaagtcg agccggtcgt acacctcgta gggctgcttc ttgcgcaggt
     1681 cccattcgac gcccgagccg cgcagcatcg gtccggtaaa accacgtgcc agcgcctgct
     1741 ccggcgacac cacaccgatg cccacggtgc gctgtttcca gatgcggttg tcggtgagca
     1801 gcgtctcgta gtcatccacg cacttgggga agcgccgggt gaagtcctcg atgaaatcga
     1861 ggagcgagcc ctggcggttg gcgttcattt cggcgagcgc gcgcgcgttg cgggactgct
     1921 gcgcctcgta cttcggcatc gaatcgggca ggtcgcggta cacgccaccc ggccggtagt
     1981 acgcggcgtg catgcgcgcg ccggacaccg cctcgtagca gtcgagcagg tcctcgcgct
     2041 cgcgaaagcc gtacaggaac acggtcatcg cgccgagatc gagccccagc gtggcgacgc
     2101 cgaacaggtg attcatgatg cgcgtgatct cgtcgaacat cacccggatg tactgcgcgc
     2161 ggaccggcac ttcgacgccg agcagcttct ccagcgacat gcaaaacgca tgctcgctgc
     2221 acatcatgga gatatagtcg agccggtcca tatagggcag cgactggatg tacatgcgcg
     2281 attcggcgag cttttcggtc gcgcggtgca gcaggccgat gtgcgggtcg gcgcgctgca
     2341 ccacctcgcc gtccaactcg agcaccagcc gcagcacgcc gtgcgcggcg gggtgctgcg
     2401 gcccgaaatt gacggtgtag ttcctgatct ccgccatcag tgccgttcct cgccgtagtt
     2461 cggctcgcgc accacgcgcg gcgtgatctc gcgcggctcg atcgtcaccg gctcgtagac
     2521 gacgcgcctt tgctcggggt cgtagcgcat ctcgacgtgc ccgctgatcg ggaagtcctt
     2581 gcggaacggg tggccgacga agccgtagtc ggtcaggatg cggcgcaagt cgggatggcc
     2641 ttcgaagacg atgccgaaca ggtcgaacgc ctcgcgctcg aaccagttca cccccggcca
     2701 cacctcgacc agcgaatcga gcatcggcag cgcatcgtcg gcgcagaacg cgcgcagccg
     2761 caggcgccag ttgtgcgtaa ccgaggtgag gtgcagcacc gcggcaaagc ggcccttctc
     2821 ccagggctgg ttgccgtacg tcgagtagtc cacgccgcag aggtcgatca gctgctcgaa
     2881 tttcaggccc ggttcgtcgc gcagcctgcg gcaggcgtcg aggtagccct ccggcggcac
     2941 ctcgacagtc agctcgccga ggcgctcgac gggcgcaaag cccgccgccg tcagcgcatc
     3001 gaaaagtctt tgcagctttg gcgtcatcgc gcgatcgtgt tggtgcgccg gatcttgttc
     3061 tgcaactgca ggatgccgta gagcagcgcc tcggcggtcg gcgggcaacc cggcacgtag
     3121 atatcgaccg gcacgatgcg gtcggcgccg cgcaccacgg agtaagaata gtggtagtag
     3181 ccgccgccgt tggcgcacga gcccatcgag atcacccagc gcggctcggc catctgatcg
     3241 tagaccttgc gcagcgcggg tgccatcttg ttgcacagcg ttccggcaat gatcatgacg
     3301 tccgcctgcc gcgggctcgg ccggaacacg atgccgaagc ggtcgaggtc gtagcgcgac
     3361 gcgcccgcgt gcatcatctc gatggcgcag cacgcgaggc cgaaggtcac cggccacagc
     3421 gagccggtgc gcgtccagtt gattaccgtg tcgagctttg cggtgacaaa gccttggggc
     3481 atgacgcctt caatgcccat gccctactcc cattccagcg cccccttcat ccattcgtac
     3541 acgaaaccga ccaccaggat gccgaggaac agcatcatgg ccacgaagcc ggcaaggcca
     3601 atgtcgttca gtgccaccgc ccatggaaac aggaacgcga tctccaggtc gaacaggatg
     3661 aacaggatcg ccaccaggta gtagcgcaca tcgaacttca tgcgcgcatc ctcgaaagcc
     3721 tcgaagccgc actcgtacgg cgacagcttc tcggggtcgg ggcggcttgg accgagcagc
     3781 ttgccgagca ccattggccc cacgccgacc gcgaggccga cgaggatgaa cagcaggatc
     3841 ggaaagtact cagcaagcat agttggtgcc cacgagcaga ctcgaactgc tacggctgtt
     3901 taggccacta gcccctcaag ctagcgtgtc taccaatttc accacgtggg cctgttattc
     3961 gagccaaatt atcctataaa aatcatttcg gtatgcccga tgccggcgag ccggcatcgc
     4021 cgcccgaagg cggcagcgcc tgcggcctgg gaatctcgcc gcccgcgggc ggcgcgggcg
     4081 gcggcttcac cgcgtccatc accgtcccgg cctcggtcct gggcttttcg ctcgcgaggt
     4141 aggccagccc gaggctggtg agaaagaaga tcgttgccag cgccgcagtc gcgcgcgaca
     4201 ggaagttcgc cgagccggtg gcgccgaaca ggctcgagga ggcgccgccg ccgaaacccg
     4261 agcccatgtc ggcgcccttg ccatgctgca gcagcaccag gccgatgatg gcgagggcta
     4321 tcagcacgtg cgcgacgatg atcaaggtaa tccactgtcc catgtcctca gcctttctgt
     4381 tccgcctctt tgcagatggc gatgaaatcc tgcgccttca gcgacgcgcc cccgatcaac
     4441 ccgccgtcca cgtcgggcat cgcgaacagc gccgcggcgt tgtcggcctt gacgctgcct
     4501 ccgtagagga tgcgcgtcgt tgccggcagg cgctttctca gcaagccgtg cacctcctgc
     4561 gcctggtccg ggctcgccgt acgcccggtg ccgatcgccc acaccggctc gtaggcgagc
     4621 acggcatcgc cgatctcgcc ccgcgccagc accgcgtcga gctggcgcgc gaccacgtcc
     4681 tgcgtgcgcc cggcttcgcg ctccggcagc gtctcgccca cgcacaggat cggcgtgatg
     4741 ccgccgctac gcgccgcagc gaacttcgcc gcgaccgccg cgtcgctttc gccgtacagc
     4801 tgccggcgct ccgagtgccc gacgaggaca tagcggcagc cgaattcccc cagcatggcc
     4861 gcggatactt cgccggtgta cgcgcccgac gcgtgctcgc tcaggttctg agccccccag
     4921 gcaatgcgcg ttccccgcaa ccgctcggcc gcttgcgcca ggtacgggaa cggcacgcac
     4981 accgcgcatt ccacgccctg cacgcccgcc acgatcgcgt ccagcagggc ccggttggcc
     5041 gccaggctgc cgtgcatttt ccagttgccg gcgacgagcc gagcgcgcat cccgtgaccg
     5101 ttcgctgaaa gcgtgaattc tacccttttt gccctgcagc atgggagccg cgcacgagcg
     5161 cacgcgccgc ctcggtcatc gcatcgcgca ggtgccgcgc agcgcgctcg cgcagcggcg
     5221 cgagcaacgc cggcaggcgc accgcgtggc tgagcaactg ggtttcctcg gcgtacaact
     5281 gcagcatctc gagcacccgg cgctcgagct cgcccggcgc gaacgcgtcg cgcgcgcgcc
     5341 ggcgctggcc ccaggcttcg agaatgcgct gcgccgtgtc cagcccgagc tcgatgcgtc
     5401 gcagccgcac cggctcgatc cgcgcgtagg ggatttcgat gcgcaccggc gcatcgccga
     5461 tgcgcgcgag gaacgcgcgg tcgatctcgc gcgccgcggc gaggagctct cgcgcgtcac
     5521 cggggctccg cccggcgccc gcgcgctgga tcagcagcgc gtccttgcgc acctccttgg
     5581 cgacgttgtc cgcaaggaag ggctcgagca cgggcaaggc gagacgcaag ggcagcgccg
     5641 cgcgcagcac cgccgtggtg cggcggctga actcccgcag cagccgcgcg ttcagcgccg
     5701 ccagtgcgtt gacccggttc aggccgcacg cggcttcacg gcgcggtgaa aaccgagcat
     5761 gcccgagaac accgtgcgcg acgtgacgtc ctggtagccg acgtcctcga gcatgtcggt
     5821 gagctcacgc gccgagtaga acatctgcag cgcctggatg aagtactcct tgcagttgag
     5881 cgccgcgggc ccgctgcgga acacgaaacc ggtgaaccac aggcaaaagc gcaggtacgc
     5941 gtagtacagg cgctcgacgg tgcggttccc ggggcgcagc atgtcgcagt ggtagaagcg
     6001 cccgccgggc ttgagcacgc gccggatctc ggcggcgacg cggcggatgc gcaggtggcg
     6061 cgacgcgaac tgcagcgtcg cgacgtcgaa cgaattgtcc ggatacggca gcgtgtgcac
     6121 gtcgtcgatc acgctctgaa tgcgaaagcc cgccgcctgg gcgcgctggc ggcccacttc
     6181 ctgcatggcg gcgctgcggt cgatggcgtg cacctcgagg gtcggctcgc gcttgagcag
     6241 cgcgatgccg atggcgttgg tgccggcgca gacatcgagc acccgctccc ccggccggat
     6301 gtcgaccgtc gccatgaact ggcgcaggaa ccagccccac aggccgaggc tggcgatgta
     6361 gttggcgcgg tcgtagtacg gggcgacgtc ggcgaacacg tcgttcagct cttgcgtcca
     6421 gacgacgttg aagcgctctt cgcgggcgat ttcccgcgtc gcaaccggcg gtggattcat
     6481 ggggttccct tcattcagat ccgggccaag taaagcaccg ctgccccgct ccctgcaagc
     6541 gcgccggcat agacgatcgc cgcgacccag gtgtcggcgc ggatgccaag gtcgtagcag
     6601 gtgatgcgca ggcggtgggc tgcgctccag agcgacagga acaccgtgcc cgccagcgcc
     6661 agcttgacca gccagtggcc ggcgaaggcg tgcaggcggt cgtaggcgag gaggtccggg
     6721 gcgtgtccga gggccgcaag caaggtgagc aggacaagca ccggcgtcag gaacgccgtg
     6781 agcgttccgc ccgccgcgaa gggaccccag acgatcggtt tgttcgatac cgccatgctc
     6841 agaatgctcc cgccagaaag aggaccgcca gcgacagcac cgcccagacg gcgaaatgcg
     6901 cgcccgagat ggccccgtcc ggcacgaacg cctcgccgcg ctgcaccggc agcgccttcg
     6961 gcgtcaggtt gaaccaggtc acgctgtggt agaccgcgaa ggcgagcgcc gcgagatgaa
     7021 acgcgatgct caccgggtgc tgcaggagct cgaggaacgc ggcccaggcc tgcggcccct
     7081 gcgccagctg cttcaggccc cagaccatca gcaatgtcca ggcgccgatg aacaggcagg
     7141 agaactcgcg caccatgtag cggatgtagc gcggatgccg gaagatccat tccgacgtga
     7201 ccgcgcgcac gtagggcttg cgtttcactt cttcagccat ggcatcaggt gctgcgtgta
     7261 ccagtccacc gtgctgcgga tctttacctg ctggatcgcg cccgccggat ccacatgctc
     7321 cgggcagact tcggagcagg cgccgacgaa cgagcactcc caggcgcctt cggtcgccgc
     7381 gatctcctgc tggcgcgcgt cgcgcccgcc gtcgcgcgaa tcctggttcc agcggtgcgc
     7441 gagcgcgatc gcggccggac cgacgtactt cgggatcaac gcgtactccg ggcaggccgc
     7501 gtagcacagc atgcagttga tgcacatgct gtagcgcttg aaccgcgcca gctccgccgg
     7561 cgtctggcgg tactcgcccg cctcgatcgc cttcgcgtgc ttgggcatca ggtagggccg
     7621 cacgcgcgcc agcttggcca tgaaatcgtc gatcaccgtc accagatcgc gctcgacggg
     7681 aaaatgcgcg agcggctcca cgcggatcac gcccgcatag tcgcgcagga acgccttgca
     7741 ggcgagcttg ggctcgccat tgatcatcat gccgcagcta ccgcacaccg ccatgtggca
     7801 cgaccagcgg tagctcagcg tcggatcgac gctttccttg acgtggttga gcgcatcgag
     7861 caccacccat tcctcgggac attcgatcgc gtagcgctgg aagcgcggct cgcggtcgct
     7921 ctccgggttg tagcgcagca cctccagctc gagcgcgcgc gtgctcattc gtggcctccc
     7981 gaataatcgc gcacgccggg ctgcgagcgc gtgatcacca cgtcgcagta gtccacgcgc
     8041 ggcgccgccg acgcctggta atacgccagc gagtggcgca ggtacttctc gtcgtcgcgc
     8101 gcggtgaaat cgagccgctg gtgcgcgccg cgcgattcct tgcgcgcccg cgcgctctgc
     8161 accaccgtct cggcgcaatc gagcatcgag cccagctcca gcgcctgcag caggtccgtg
     8221 ttgtagacgt tcgtcttgtc gtgcagctcg atgccaccgt agcgccggcg caggtcggcg
     8281 agcttggcgc aggccgcctc caggccctcg ccgctgcggt agatgccggc gtgctgttcc
     8341 atcgtgtcca tcatctcctt gcgcagcccg gcgaccgtat cgcggcggcc cgtcctttgc
     8401 accagctcgc gcacgcgccc ctgcgcggca gccgcgagcg ccgcgagtgc ggcgtcgccc
     8461 gccggccgtg cgcctgcgcc cacgccaacg tactgcatcg cgctctgcgc cgcccggcgg
     8521 ccgaatacca ggagctcggt gagcgagttc gagcccagcc ggttggcgcc attgatgctg
     8581 acgcaggcgc actcgcccgc cgcgaacagc cccgccagcg gcgttgccgc gttgatgtcg
     8641 gtatggatgc cgcccatcat atagtgcacc accgggcgca ccggcaccgg atcggtcacc
     8701 ggatcgaccc ccatgtagct gatcgacatg tcgcgcacca gcggcaggcg ctcgttgatc
     8761 ttcttctcgc ccaggtggcg catgtcgagc agcatgcact cgccccactg ggtcggcacc
     8821 atgcgcccct tcttctgctc ttgccagaat gcctggctga cgcggtcgcg cggtccgagc
     8881 tccatggtct tgagcaccgg cttgccgagg ggcgtctccg gccccatgcc gtagtcctgc
     8941 aggaagcgcc gaccgtcctt gttgaccagc accccgcctt cgccgcggca ggcctcggtc
     9001 agcagcgtgc cggtgctggg cagcccggtg gggtggtact gcacgaactc catgtccttc
     9061 agcggcacgc cggcgcggta ggcgagtgcc atgccgtcgc cggtcttgat ggcgccgttg
     9121 gtggtaaagg gaaacatgcg ccccgcgccg ccgccagcaa tgatcaccgc ccgggcctgg
     9181 aagcagcgca tctggccgct gcgcatctcc agggcggtga ggcccgcgca gcgcccgtcg
     9241 tcgaccagca gctcggtcac gtagaactcg tcgtagcgct ggatggaggg gaactgcagc
     9301 gacgtctgga acagcgtatg caggatgtga aaacccgact tgtcggcggc gaaccaggtg
     9361 cgctgcttct tcatgccgcc gaagggccgc actgccaccg cgccgtcggc ttcgcggctc
     9421 cacggacaac cccagtgctc gagctggatg agttctccgg gcgcctcgcg gatgaaatag
     9481 tccaccgcat cctgatcgct cagccagtcg ccaccgccca ccgtatcgtt gaagtggtgc
     9541 tcgaggctgt cgtcgggctt gatcacgccg gcggcgccgc cctcggccgc gcaggtgtgg
     9601 ctgcgcatgg gatatacctt ggaaatcagg gcgatgcgca aggacggcgc ttcctgcgcc
     9661 agagcgatgg cggcgcgcag cccggcgcct ccggcgccga tgatcgccac gtccgtgcgg
     9721 atgatttcca ctgccggcat cctgcccttg cggcgctcgc gcatgcttga cctgcgtcaa
     9781 acgtctgcga accggccggc gcctagccga agcgcccggt gatgtagtcc tcggtctgtt
     9841 tcttcctcgg cttgataaag atctcgtcgg tcacgccgta ctcgatcagc tcgccgaggt
     9901 acatgtaggc ggtgtagtcg gagacgcgcg ccgcctgctg catgttgtgc gtgacgatga
     9961 ggatcgtcac cttgttcttc agatcgtgga tcagctcctc gatcgaggcc gtggcgatcg
    10021 ggtcgagcgc cgaggtcggc tcgtcgaaca ggaggatctc cggatcggtg gcgagcgcgc
    10081 gcgcaatgca caggcgctgc tgctggccgc cggacaggtt gaaggcgggc tcctgcagcc
    10141 ggtccttgac ctcgtcccac agcgcggcgt tgcgcaacgc ctcctcgacc ttctcgttca
    10201 gcgtgacgcg ccggccctcg ccgcgcaccc gcagcccgta ggccacgttc tcgtaaatcg
    10261 actttggaaa cggattgggc ttctggaaca ccatcgagat gcgcatgcgc acctcgatcg
    10321 ggtccaccgc gtgcgccagg atgttgacat cgtccgggta cagtcggatt tcgccctcgt
    10381 agcggttgcc ggggtacaag tcgtgcatgc ggttgaagca gcgcaggaaa gtcgacttgc
    10441 cgcagcccga gggaccgatc agcgcggtca cctgcttctc gtagaccggc atgctgagcg
    10501 tcttcagcgc ctggaagctg ccgtagtaga agttgagatc cttgaccgcg gccttggctt
    10561 gcgccgcggc ggcgagctgc gacggcaccg ctaccatttg attcgcttcc tcagtctgta
    10621 gcgcagccag atggcgatgg cgttcatgcc gagtgtcatc agcacgagga caaagcccgc
    10681 ggccgcggcg ttctggtgaa aggcggcctc ggggcgcgaa gtccagttga acatctggat
    10741 cggcatcacc gtgaacggcg acatcaccca gtcgaacgac acgaacgggg gctccgcctg
    10801 caccggcgac ggcggcagga aggcgatgaa ggtgagcgcg ccgatcgtga tgatgggcgc
    10861 ggtctcgccg atggcgcgcg ccatgccgat gatcacgccg gtcaggatac ccggcatcga
    10921 gtacgggatg atgtggtcct tgcacacttg ccactgggtt gcgccgagcg cgtaggcgcc
    10981 ctcgcggatg ccctgcggga tcgagcggat cgcctcgcgc gtggcgacga tcactaccgg
    11041 caggatgagc agcgccagcg tcagccccgc cgagaggatc gactgcccga agccgaactg
    11101 gtacacgaac aggccgagag cgagcaggcc gtagacgatc gagggcacgc cggcgaggtt
    11161 ggtgatgttg atctcgatca catcggtgac ccagtgcttg gccgcgtatt cttccaggta
    11221 cacgcccgcc gccacgccga gcggcacggc gacgaacgcc gtgaccagca tcaccagcgt
    11281 ggagcccacc caggccgaga ggatccccgc ctgccctgcg cgccgcgatg ggaagttggt
    11341 gaagaaatcg ccggtgaggc gcggcacgcc gtcgagcgcc atgtcgatga acagcgcggc
    11401 gaaggtgagc acgccgagca tcagcgcgag cacgccgacc acgccgaaga tcgcgtccca
    11461 gcgcttgtgc gctgcgatca gcctgcggat ctcttccagg cgccgggcgt ggggcatcaa
    11521 taggcctcgc ggaagcgctt gcgcagcacg tggccgccga tgttgaacgc cagcgtcacc
    11581 aggaacagcg tcagcccggc cgcgaagatc gtctggtagc ccactgagcc gtgcggcagg
    11641 tcgccgagcg ccacctgcac gatgaacgcg gtgatggtcg cggccggctc gagcggattg
    11701 aacgtgaggt tgggctgcat gcccgcggcc accgccagga tcatggtctc gccgaccgcg
    11761 cgcgagatgc cgaggatata ggccgaggcg atgcccgaga tcgccgccgg caccacgacg
    11821 cgcgtcgcgg tctggaagcg cgttgcgccc atcgcgtacg agccttcgcg caggctcatc
    11881 ggcaccgcgc gcatcgcgtc ctcggcgatc gagctcacgt acgggatgat catgatgccc
    11941 atcacgattc ccgcgccgag caggttgaag ccgggcagct ccgggtagat tgtctgcagc
    12001 agcggcgtga cgaagagcag cgcgaagtag ccgtagacga tggtcggcac cccgcccagc
    12061 atctcgagga acggcttcgc cacctcgcgc acgccgaacg gcgcgaactc cgacaggtag
    12121 atggcgatca cggtgcccac cggcacggcg atgccgagcg ccaccatcga gctggtcagc
    12181 gtccccgaca acaacaccat gatgccgaag tgcgcgtcgt cgaacagcgg cgtccactgc
    12241 gtgtcggtga ggaagtccca cagggggacg tggctgaaga acaccaccga ctccttcacc
    12301 agaacgtaga tgatgcccac ggtggtgaac acggacacca gcgcggcgag gaacagcgcg
    12361 ccctcgatca cgcgttcgcg cgcaatgcgc gcgcgccgcc gctcgagccc cgccctgacg
    12421 tctggtgcca ccacggtgcc ggagtctaca gcttcgcctc gcgccgcagc agttcctcga
    12481 tggtgacgcc cacttcggcg acacccccga acaccgtgcc cttcttgccc ccggtcacat
    12541 gctgccaggc ttgctggtag gccgactccg gcagcggtac gtacttgact tcctgcgcca
    12601 gcttcgcgcc gtgcgtcatg tagtaccgta cgaactcctt gacctcgggc ctggcgagcg
    12661 acttggcgct gacgtagatg aagatcgggc gcgcgagcgg cgagtagctg cctttcacca
    12721 ctgcctccag cgagggcccc accggccggc ccttttcgtt cacgatcggc accgctttca
    12781 gcttgtcctt gttctccacg tagtaggcgt agccgaagta gcccaggccg ttcacgtcgc
    12841 gcgacacgcc ctgcaccagc acgttgtcgt cctccgacgc ggtgaagtcg ccgcgcgagg
    12901 acttcgcccg gccgaccacc gcctcggtga agtagtcgaa cgtgcccgag tccgcgccgg
    12961 gtccgaacag cttcatcggc tccgccggcc aggccgggtt gacgtgattc cattgcgtca
    13021 ccgtgttctg cgccgacggt tcccacatct tcttcatctc ggcgatcgtc agctgctgga
    13081 tgaacttgtt cttcgggttg accaccaccg tgatcgcgtc gaacgcgacc ggcagctcga
    13141 agtactcgac gccggccttg cggcaggcct gcatctcttt cttcaggatc gggcgcgatg
    13201 cgccggtaac gtctatctcg ccgcggcaga acttcttgaa gccgccgccc gtgcccgaga
    13261 tgccgacggt gacccggatc gcgccgcgct tggacttctg gaaatcctcg gccaccgcct
    13321 cggtgaccgg gaacacggtg ctcgagccgt cgatcttgac cagcggctgt gcaagcaccg
    13381 gcgcggacgc gagaaaggcg gcagcgactg ctagggttct catctattcg ctcctgtcca
    13441 tgattgacgg gcgagtctag gaggggtttg tgacaccccc attacaggcg gccgatctcc
    13501 tcgagccttc ttctcagcac cagccggtcg agcctgtcga tcatgcgggg accctttcgt
    13561 accagcggcg ggtgccattg acgacgtaga caactgagag catcaccggc acctcgacca
    13621 gcacgccgac cacggtcgcg agcgccgcgc cggaatcgaa gccgaaaagc gtgattgccg
    13681 cggccaccgc cagctcgaag aagttgctcg cgccgatcaa cgccgacggt ccggcgacgc
    13741 tatgcgcgac acgcaatcgc cggttgagca ggtacgcgag ccccgagttg aagtacacct
    13801 ggacgaggat cggcacggcg aggatggcga tgatgagcgg ctgtgcgagg atctgccggc
    13861 cctggaaggc gaagagcagc acaatggtcg cgagcagcgc cgcgaccgac cagggctgga
    13921 tgcgcgcgag cgtcgcctgc aggcgtgcct cgccgcgctt cagaagggat gcacgccaca
    13981 gctgcgcgag gatcaccggg acgacgatgt agagcacgac cgaggtaagc aacgtgtccc
    14041 agggcacgac gatcgccgaa agaccgagca ggagcgcgac gatcggcgca aacgccacaa
    14101 ccatgatggc gtcgttcagc gccacctggc tgagcgtgaa gtacgggtcg ccctgcgtga
    14161 gccggctcca cacgaacacc atggcggtgc acggcgcggc cgcgagcagg atcagtcccg
    14221 cgacgtagct gtcgatctgc tccgccggca gccacggcgc gaacacgtgg cgcaggaaga
    14281 accagccgag gagcgccatg gaaaacggct tcacaagcca gttgaccgcg agcgtcacgc
    14341 cgataccgcg ccaatgcctg cgcacctcgc ccatcgccgc gaagtcgatg cgcagcagca
    14401 tggggatgat catcacccag atgagcaccc cgaccggcag gttgacgctc gcgacttcca
    14461 tgcgcccgag cgcctcgacc ggtgcgggga gccaatgccc tagggcgacg cccgcgacga
    14521 tgcacaggag cacccagagc gtcagccagc gctcaaacag gctcatctca agcggtcata
    14581 gggacgcaac aggcggcctt cgcctccttc acctcgacgc gcgtgtcctc accgtagcgc
    14641 ggcgcggcct ccagcgtatg gaacgactcc cacgcaatgc cctgcgggtc gagcgtccag
    14701 tgcttgtcgc tcttcgcgta gcagcaggtg gcgttcttct ggtccatgac cgcgacgtcg
    14761 gcctgcgcga gctgcgagcg cagctggccg agctccgcgc cgtcgtccac ctggatgccc
    14821 aggtggtcca ggcccgcctt cgcgccccgc ttcgagatcg cgaagttgac gcgcgggtcc
    14881 tccagctgcc acttggcgtg gtcgtccttc ttcacggtag gctcggcatc gaacaaggcc
    14941 ttatagaagc ggatgctctg ctcgaggtca tgcacggcga cgtgtacgtt taggcgcttc
    15001 atctttctct ccggtggatg ggttgcaggc agcgccgccg cagcagttct cgccgaggaa
    15061 accgacgagc gcgttcatgg ccgtgaaatc ggccgagtag atgacgaagc ggccttcctg
    15121 gcggctctgc gccagcccgg cgcgggtcag ccccgcgagg tggaaggaca gcgtggcggg
    15181 cgccagatcg agctgctcgc cgatctgacc cgccgacatg ccttcgggac cagcttgcac
    15241 cagcagccgg aagatggcga ggcgcgtgtc ctgcgccagc gcgctcagcg cttggacggc
    15301 ctgcttcgtt tccatgcttc cactattatg gaattgatgg tcaaatagca agccgctaac
    15361 cggtcgccgc gcggcgcacc gcatccgcga ttgatttcgc tgccgcttca acggtttgcc
    15421 tgctctctcc ttccaccatc acccgcagca gtggctccgt gcccgaggga cgcaggagca
    15481 cccgcccctt cccgtccagc gacttttccg cctcaacgcg cgccgccgcg atcgcccggt
    15541 ccttctgcca gtcgaagccg cgcggcacct gcacgttgac gaggacctgc ggaaacatga
    15601 ccaggtcgcc cgtgagctcg ccgagcgtct tcccggactc gcgcatcgcc gccagcacct
    15661 ggagcgccga cacgatgccg tcgcccgtgg tatgccggtc gaggcagatg atgtggcccg
    15721 agttctcgcc gccgagctgc cagccccttt cgcgcatcag ctccaggacg tagcggtcgc
    15781 ccaccttcgc gcgcgcaaaa gcgaccccca ggcgcgcaaa tgcctgctcg agcgcaaggt
    15841 tggtcatcag cgtgccggca acgcccgcga ccctgcctct ggcggcgcga tgccgtgcga
    15901 tggcgtagag caactggtcg ccgtcgtaga gcgcgccctg cgcgtccgcc atcaccagcc
    15961 ggtcgccgtc gccgtcgagc gcgacgccga ggtcggcctc gtggcgcatc accgccgcct
    16021 gcagctgcgc cggcgaagtg gcgccgaaga ggtcgttgat attgaagccg tcgggcgaga
    16081 ccccgaccga gaccacgtcc gcgccgagct catggaacac gtgcggcgcc acgctgtacg
    16141 ccgcgccgtg cgcgcagtcg accacgatgc gcagcccgcg caggtcgaga tccgcgggaa
    16201 acgtggactt gcagaactcc acgtagcgcc ccccggcgtc gtcgacgcgc cgcgcgcgcc
    16261 cgaggtgcgc cgaggcgttg cagccgatcg gctgcgccag cagctcctcg atttcggatt
    16321 ccaccgcgtc ggggagcttg aagccgtcgc cggagaagaa cttgatgcca ttgtcgccgt
    16381 aggggttgtg cgaggcgctg atgacgacgc cggccgccag ccgcagcgcg cgcgtgaggt
    16441 acgccacccc cggcgtcggc agcggcccgc acaggagcac gtccacgccg gccgccgaaa
    16501 agcccgcctc cagcgccgcc tcgatcatgt agccggagat gcgcgtgtcc ttgccgatca
    16561 gcacgccggg gcgatcgccc gcgccggcct cgcgggccgc gagcactttg cccgcggcgt
    16621 agcccaggcg cagcacgaag tccgcggtca tgggcgcctc gcccaccgtg ccgcgcacgc
    16681 cgtcggtgcc gaaataggtt cgcgtcatgc cttgcgccag gcgtcccaga ccaggatcac
    16741 gtcgcgcgtc tccttcacgt cgtgcacgcg cagtattctc gcccctccga ggagcgcgag
    16801 caaagccgcg gcgaggctcg ccggcaggcg cccggcggca tcgcgcccgg tgatgcggcc
    16861 gagggcggac ttgcgcgacc agccggcaag gaccgggacg ccgaggcgcg caaactcggg
    16921 caggcgccgc agcagcgtca ggttgtgctc gacggtcttg ccgaaaccga agcccggatc
    16981 ggcgacgatg cgatcgcgcg caatgccggc gcgctcgcag gcggcgatgc ggccggcgag
    17041 gaactcgccc acttcgccca ccacgtcgtc gtagctcggt tcatcctgca tcgtcgccgg
    17101 ctcgcccttc ttgtgcatca ggcacacggc gcaatcgctt gccgcgacgg cctccagcgc
    17161 gcccggcgcg gcaagggcct cgatgtcgtt gatcatgctg gccccggccg ccagcgcggc
    17221 gcgcatcacg ccggggcggc gggtatccac cgacaccggc acgccacgca tacgctcgat
    17281 caccggcagg atgcgatcca gttcctcgcg ctcgtcgacg ggcgccgcac cgggccgcga
    17341 ggattcgccg ccgatgtcga tgatgtcggc gccgtccgcc accagccggc aggcgtgcgc
    17401 gaccgcggcc tcggagtcga ggaaccggcc gccgtcggag aaggagtcgg gcgtgacgtt
    17461 caccacgccc atcaccaggg cgcggtcgag cggcaggggg aacctgccgc agcgaagcgt
    17521 cacaccgcag gggacgcggg agccgtggtg ccggccgcgc cgtcgttggc ggcgccgccc
    17581 gtcggcgtcg tggtcgtcga agtcactttc ggcggccgcg gcggcctgcc ttgcatgacg
    17641 tcgttgatct gatccgcgtc gagcgtctcc cactcgagga gcgccttggc catcgcctcg
    17701 accttgtcgc ggttctcctc gatgagcttg cgcgccagct tgtactgcgt gtcgatgatg
    17761 cgccggatct cggcgtcgac cttctgcagc gtcgcctcgg acacgttctt gtgcgtggtg
    17821 atcgagcggc cgaggaacac ttcgccctcg ttctcgccgt acaccatggt gccgagctcg
    17881 tccgacatgc cccactgcgt caccatgcgg cgcgcgatct cggtggcgcg ctcgaagtcg
    17941 ttggaggcgc cggtggtcat ctggtgcatg aagatctctt cggcgatgcg cccgccgaac
    18001 agcaccgtga tggtgttgag caggcggtcg cgatcctggc tgtagcggtc ctgctccggc
    18061 agctgcatgg tcacgccgag cgcccgaccg cgcggaatga tggtcacctt gtgcaccgga
    18121 tcggtcttcg gcagcaggcg cgcgaccacc gtgtgcccgg attcgtggtc cgcggtattg
    18181 cggcgctcct cctcgggcat caccatcgag cgccgctcgg cgcccatcat gatcttgtcc
    18241 ttggcgcgct cgaagtcgtc catgtcgacc aggcgcttgt tggcgcgcgc cgcgaacagc
    18301 gccgcctcgt tcaccaggtt ggcgaggtcc gccccggaaa aaccgggggt gccgcgcgcg
    18361 atgatgtcgg ccttcacgtc gggcgcgagc ggcaccttgc gcatgtgcac cttgaggatc
    18421 tcctcgcggc cgcggatgtc cggcagcggc accacgacct gccggtcgaa gcggcccggg
    18481 cgcatcagcg ccgggtcgag aacgtcgggg cggttggtgg cggcaatcac gatcacgccg
    18541 gcggtggcct cgaagccatc catctccacg agcagctggt tcagcgtctg ctcgcgctcg
    18601 tcgttgccgc cgcccaggcc cgcgccacgc tggcggccga ctgcgtcgat ctcgtcgatg
    18661 aacacgatgc acggcgcgtg ctttttcgcc tgctcgaaca tgtcgcgcac gcgcgccgcg
    18721 cccacgccga cgaacatctc gacgaagtcg gagcccgaga tggtgaagaa cggcaccttg
    18781 gcctcgccgg cgatcgcgcg cgcaagcagc gtcttgccgg tgcccggcga gccgaccatc
    18841 aggacgccgc gcgggatgcg cccgccgagc ttctggaatt tggaggggtc gcgcaggaaa
    18901 tccaccagct cggagacctc ttccttcgcc tcctcgcagc cggcgacgtc ggcgaaagtg
    18961 acggtgttgg tcgactcgtc cagcatgcgc gccttcgact tgccgaagga gaacgcgccg
    19021 ccgcgcccgc cgccctgcat ctggcgcatg aagaagatcc acacgccgat caggagcagc
    19081 atcgggaacc acgaaacgaa gatgttcatg aggagcgagg gctcctcttc gggcttggcc
    19141 tcgatcttga cgccgtactt gagcaggtcc gacaccagcc agatgtcgcc cggcgagtag
    19201 ctggtgacgc gcttgccctc ggcggtggtc gccttgagct ggcggccctc gatcgtgacc
    19261 ttggagatgc gcccggattt cacctcctcc aggaattgcg agtactccat cggcgcctgg
    19321 gtcacctggc gggtgttgaa ctggttgaac accgtcatca gcacgaggcc gatcaccagc
    19381 cagatgacaa ggttttttaa catgttgttc aaggacttgc tcctatgttg gggcagcgcc
    19441 gagaaggttc gagtttacgc ccaatcgcta cattgcggcg ttcccgcccg ggttcaatgg
    19501 cttatgacac cgcgtgcgag caaataattc tctctggatt cccctcgcga ggcggcgggc
    19561 ttgcgcaccg cgaccgtttg aaacgacgcc ttcagcgccg cccgcacttc gtcgaaggct
    19621 tcgccgtgga acatcttggc gaggaagacg ccctccggct tcagcacctt gccgcacagc
    19681 tcgatcgccg cgcgcagcag ctcggcggcg cgcgcctggt cgaccagggg gaccccggtg
    19741 atgttcggca tcatgtccga gagcaccacg tccgccttgc ccccgagcgc gcccgccagg
    19801 tccgcttcgc ggaaatccgc cttgaacacc gtgacgccgt ggatcggttt catctccagc
    19861 agatccacgg cgaccaccac gccgcccggc cgcacccgat cggcggcgac ctgcgaccag
    19921 ccgccgggcg cggcgcccag atcgagcacg cgcatgccgg gcttgagcag gcgctccttg
    19981 gcatcgatct cgagaagctt gtaggccgcg cgcgagcgat agcccgcctc cttcgccttg
    20041 cgcacgtagg cgtcggtgac gtggcggcgc agccaggctt tgctcgactt ggccatcggc
    20101 gctgatctta tactcgcgcc atggaccgaa agctgctcaa ggcccgcgcg cacgcgctgc
    20161 accccatcct gcagctcggc gagaagggcc tcaccgacgc cgtcgttgcg gaaattgacc
    20221 gcgcgctcgc ccaccacgag ctgatcaagg tgcgcgccgc gccgctcaat cgcgacgcgc
    20281 gcgaagtggc gctggcgagc atttgcgagc gcacgggcgc cgcggctgtg cagcacatcg
    20341 gcaagatctt tgttctgttc cgagagaaac ctatcgaggc gcagcctgat gacccggtcc
    20401 ggccaccggc gcgcgcccgg gcagcaccgc ctggccgacg tcggagcgaa aagcgcgcgc
    20461 gtaggtcgcc aggacgcggc cgtcggcgga atcgatgagc cggacttcga cgcggtaatc
    20521 gcgctcggct ttgacgccgc gaagcggcgg cgagcgaacg acgcacgctg cttcggccag
    20581 ccggtagttg caggcgaagt ctgcgccgtg gaactcgagg tacggtccct cgccctccgt
    20641 gcacgccacc gccaggacga gcacgagcgc cgcgaggagc ctactcgtag cgtacgtcca
    20701 ggatctcgta cttcttgatt ccgcccgggg tgtgcacgtc gacctggtcg ccggcttcct
    20761 tgccgatcag cgcgcgtgcg atcggcgagg agatcgagat gcggccggcc ttgatgtcgg
    20821 cctcgtcctc gcccacgatc tggtaggtca ccggctctcc gccctgctcc tcgagatcga
    20881 cggtggcgcc gaacacgcag cgcccgtcgg cgtcgagcag cttcgggtcg atgatctgcg
    20941 cgttgccgag cttcgcctcg atctcggaga tgcggccctc gatgaagccc tgctggttgc
    21001 gcgccgcgtc gtactcggcg ttctccgaga ggtcgccgtg cgcccgcgct tccgcgatcg
    21061 cctgcgaatt ggcgggccgc tcgacggtct tcagccggtg cagctcggtg cgcagcgcct
    21121 cggcgccgcg cgtcgtcatg ggggtcttgg ccatatcagg aagtggatgc taccgtgcgg
    21181 gtcaggggag ccgccccgag tttcgcatgc aggtcctgca ggcggtaggg ctcgaggcgg
    21241 ggcaggtact tcatgccggc gcaggcggct ttggcgccgg cgatcgtggt gtagtaggtg
    21301 acgcgcgctt gcaacgccgt ggtgcggagg gagcgcgagt ccgagatcgc ggtacgcgtg
    21361 tcctccacgg tattgacgat gaagctcacc tcgccgttct tgatcatgtc gacgatgtgc
    21421 gggcggcctt cggccacctt gttcaccggg gttgcttcca ccccggccgc cgccagcgcc
    21481 gcagcggtgc cacgcgtggc aaggaccgtg aagccgagct cgacgagctc gcgcgccacc
    21541 gccaccgctg ccgacttgtc gccgtcccgc acgctgatga acgcgcggcc gcccttgggc
    21601 aggcgcacgc cggcggcgag ctgcgacttg acgaaggcct cgccgaagga ttgcccgact
    21661 cccatgacct cgccggtcga cttcatctcc ggcccgagga tggtgtcgac gcccgggaac
    21721 ttgctgaacg ggaacaccgc ctccttcacc gagtaatacg gcggcgtgat ggtgccgcgg
    21781 atcccctgcg ccgcgaggct gatgcccgcc atggcgagcg cggcgatctt ggcgagcggc
    21841 cgcccggtgg ccttcgaaac gtacggcacg gtgcgcgaag cgcgcgggtt gacttcgagc
    21901 acgtacacaa cgccgccctg aatggcatat tgcacgttca tcagcccgcg cacctgcagc
    21961 gcgcgcgcca tgatttcggt ctggcgccgc agctcctgca ccatctccgg cgcgagcgaa
    22021 tggggcggca ggcagcaggc cgagtcgccc gaatgcaccc cggcctgctc gacgtgctcc
    22081 atgacgccgc ccaccagcac ctcgtcgccg tcggcgatgc agtccacgtc cacctcgatg
    22141 gcgtccgaca ggtagcggtc gagcagcacc ggggcatcgt tggacacctt gaccgcattc
    22201 ttcatgtagc gctcgaggtc ggcctgccgg tgcacgatct ccattgcgcg cccgccgagc
    22261 acatagctcg ggcgcaccac caccgggtag ccgatctgct cggcgagctt cacggcgtcg
    22321 gccgccgagc gcgcggtccg gttcgggggc tggcgcagct tcagtttctc gagcagcttc
    22381 tggaagcgct cgcggtcttc ggcgatgtcg atcgagtcgg cgctcgtgcc gatgatcggc
    22441 accccggcgg cagcgaggtc gcgcgcgagc ttcaacgggg tctgcccgcc gtactgcacg
    22501 atcacgcccc agggcttttc cttgtcgacg atttccagga cgtcctcgag cgtgagcggc
    22561 tcgaaataca ggcggtccga ggtgtcatag tcggtggata cggtctccgg attgcagttg
    22621 accatgatgg tctcgaagcc cgcttcgcgc agcgcgagcg ccgcgtgcac gcagcagtag
    22681 tcgaactcga tgccctggcc gatgcggttc ggcccgccgc ccagcaccat gaccttgcgg
    22741 cgcgcagtgg gctcggcctc gcactcctcc tcgtaggtcg aatacatgta ggcggtcttc
    22801 gtcgcgaact cggcggcgca cgtgtcgacg cgcttgtaca ccgggcggat gccgagcttc
    22861 ttgcgcttct cgcgcaccgc catctcgccg gtattgaaca ggtacgccag ccgccggtcc
    22921 gagaagcccc ttcttttcag catgcgcagc gtgtcagcgt cgacgtcggc gagcttctgg
    22981 tcgtccaggc gcatctcgag gtcgacgatc tccttgatct gcgcgaggaa ccacgggtcg
    23041 atgcgcgtca ggcggtgcac ctcttccagg ctgaagccgt tctcgaacgc atcgcccacg
    23101 taccagatgc gctccgggcc gggctcgccc agctcgcgct cgatcgtttc gcggtcggtg
    23161 gtgcgctggt tcaggccgtc gacgccgatc tccaggccgc gcatcgcctt gtgcagcgac
    23221 tcctggaacg tgcgcccgat cgccatcacc tcgcccaccg acttcatctg cgtggtcagg
    23281 cggtcgttgg cctgcgggaa cttctcgaag gcaaaacgcg gcaccttggt gaccacgtag
    23341 tcgatggccg gctcgaacga tgcgggcgtc gcgccgcccg tgatgtcgtt cttcagctcg
    23401 tccagggtgt agcccaccgc aagcttcgcc gccaccttgg cgatcgggaa accggtggct
    23461 ttggaggcca gggccgagga gcgcgacacg cgcgggttca tctcgatcac cagcaggcgc
    23521 ccgtcctccg ggctgatggc gaactgcacg ttcgagccgc cggtgtcgac gccgatctcg
    23581 cgcagcaccg cgatggaggc gtcgcgcatg atctggtatt ccttgtcggt gagcgtctgc
    23641 gccggcgcca cggtgatcga gtcgccggtg tgcacgccca tcggatcgag gttctcgatc
    23701 gagcagacga tgatgcagtt gtcggcccgg tcgcgcacca cctccatctc gaactccttc
    23761 cagccgatca ccgactcctc gatcagcagc tcgttggtgg gcgaggcttc cagcccgcgc
    23821 tcgcagatgg cgacgaactc ctcgcggttg taggcgatgc cgccgccggt gccgccgagc
    23881 gtgaacgagg gccggatcac caccggaaag ccgaggctcg cctgcacctg cagcgcttct
    23941 tccatcgagt gcgcgagcga ggagcgcgcg gaggcgaggc cgatgcgcga catcgcctgc
    24001 ttgaacttct cgcggtcctc ggccttgtcg atcgcctcgc gcgaggcgcc gatcatctcg
    24061 acgccgaact tttccagcac gccttcgcgc gcgaggtcga gcgcgcagtt gagcgcggtc
    24121 tggccgccca tcgtcggcag cagcgcgtcg ggccgctcct gttcgatgac gcgcgccacc
    24181 gtctgccagg tgatcggctc gatgtaggtg gcgtccgcca tttccgggtc ggtcatgatg
    24241 gtggcggggt tggagttcac caggatcacc cggtagccct cctcgcgcag cgccttgcag
    24301 gcctgcgccc cggaatagtc gaactcgcag gcctggccga tgacgatcgg gcccgcgccg
    24361 atgatcagga tcgagtgcag gtcagcgcgc ttgggcatcg gccatcagct tggcgaaacg
    24421 gtcgaagagg taggcgatgt cgcgcggccc cggacttgcc tcggggtggc cctggaagca
    24481 gaacgccggc cggtcggtgc gcgccaggcc ctgcagcgac ccgtcgaaca gcgacacgtg
    24541 ggtcggccgc aggctcgggg gcaggcttgc cgggtccacg gcgaagccgt ggttctggct
    24601 ggtgatcacc acctgcccgg tgtcgaggtc cttcaccggg tggttggcgc cgtggtggcc
    24661 gaacttcatc ttcaccgtct tcgcgcccga ggcgaggccc atcagctggt gcccgaggca
    24721 gatgccgaac acggggatgc gggtcgcgtc gaggatctcg ccgatcgcct cgatggcgta
    24781 gtcgcagggc tcggggtcgc ccgggccgtt ggacaggaac acgccgtcgg gcttcaggcg
    24841 cagcacctcg cgcgccggcg tctgggccgg caccacggtg acgcgcgcgc tgcgctccgc
    24901 gagcaaccgc aggatgttgc gcttgatgcc gtagtcgtag gcgaccacgt ggtgcgaggg
    24961 attgtcgatc aagcggtagc ccttgccgag ctcccagccg ccctcgcgcc attcgtaggg
    25021 cgcgcgcacc gtgacctcct tggcgaggtc caggccggcc agccccggga aagcgcgtgc
    25081 cgcggccagc gcccgcgcgt cgttcaccgc gcccgacatg acgcacccgc tctgcgcgcc
    25141 cttctcccgc aggatgcgcg tgaggcggcg cgtatcgatg tcggcgatgg cgacgacgtt
    25201 gcgctcggcg aggtagtccg gcagcgtctg gcgcgcgcgc cagctcgaca tcagcgcggg
    25261 cacgtcccgc acgacgaggc cggcggcaaa taccttgtgc gactcctcgt cctcggggtt
    25321 gacccccgta ttgccgatgt gcggataggt cagcgtgacg atctggccgg cgtaggaggg
    25381 atcggtgagg atctcctggt agccggtcat ggcggtattg aagacgacct cgccggcagc
    25441 gaggcccggg gcgcccacgc cgcggccccg aaacaccgtc ccgtcggcga ggacgaggac
    25501 ggcggaggcg agcgcaggca aagcgaaact cccggattag atgtgaaaaa gcggaagagg
    25561 cttgcgcccc tcccgctcgt cagagcgccg aattataccg tgccggctac ttcaggccaa
    25621 gcacgtcgaa catgtcgtac aggccgggcg ggcggccctg cagccacttc gcggcgcgca
    25681 gcgcccccgc ggcgtaggtc aagcggctct gggagcgcac cgtgatctcc acgcgctcgc
    25741 cgctgccggc gaagatcacc gtgtgctcgc cgacgatgtc gccgccgcga atggcatgaa
    25801 tgccgatggc gcgcgccggg cgctctccgg tatccccgac gcggccatgc gtcgccactt
    25861 tgcggatgtc gcgccccagg gcggtcgcga ccagctcgcc gagcttgagc gccgtacccg
    25921 aaggggcgtc gaccttgtgg cgatggtgcg cttcgacgat ctcgacgtcg taagcgtccc
    25981 ccagcgccgc tgccgccgtc tgcgccagcc ggaacaccac attcacgccg atggcgaagt
    26041 tcggcgacag cacgatcggc aggcggcgcg cgccctcggc aatgcgcgcc ttctgcgctt
    26101 cgctgaagcc ggtggtgccg atgaccatcg ccttgccgtg cttctggcac gcctcaaggt
    26161 gcgcgagcgt gccttcgggg cgcgtgaaat cgatcagcac gtcggcgccg gccagcgcgc
    26221 cgagatcggc gccgaccttc accccgcccg ccgcccggcc cagcgccggg ctgcccgccg
    26281 cgtcgagcgc cacggccaat tcgaacccgc tgtccatgag caccgcgtcg atcagcgcat
    26341 atcccatgcg gccgcccgcg ccggcgatcg caactttcat cgcgcgggct ccgccgcagc
    26401 cgccgccacc acatcgccct cgacgcgcgc cagcttgtcg tcctcgaaga acacggccag
    26461 acggcgctgc acgcgcggct ggccgggccg gtccagcagg tacacgtagt cccagcggct
    26521 ggcgtggaag atgtccgtga ccagcggcgt gccgagcacg aagcgcacct gctccttggt
    26581 catgccgcgc ttgagctgcg cgaccatctc ctgcgacacg aagttgccct gctggatgtc
    26641 gatgcgatag ggcgcgagcc agccgcagcc ggccagctgc agcgcgagga gcaagaggag
    26701 tacgcgaagc atcggcatat aatgataatc gttatgagtt cggcgcaaag cctcaaagat
    26761 atcggcctca aggccaccct gccccggcga aaggtgctgg agctgttcga gaacagcaag
    26821 gtgcgccacc tgtccgccga ggacgtgtac cgcagcctga tggccgaggg catcgatatc
    26881 gggcttgcca cagtctaccg cgtgctgacg cagttcgagc aggcggggct gctgtcgcgc
    26941 cagcacttcg agaccgggaa ggccgtgttc gagctgaacc agggtgggca ccacgatcat
    27001 ctcgtgtgcc tgcagtgcgg gcgcgtggag gagttctacg acgcggagat cgagcagcgc
    27061 cagagcgagg tggcgcgcaa gcgcggcttc gagctgcgtg gccactcgct cgcgctatac
    27121 gccgactgta ctaaagagga ctgccctaac cgcaaggacg ggcgcctcta gtccgcgtcg
    27181 agcagctccg ccgcgtgctt gcgcgtggtg gcggtgatct cggcgccgcc cagcatgcgc
    27241 gccagctcct ccacgcgcgc cgcacggtcc aggcgcgcca cgctggtcgc caccttgccg
    27301 cgcgcgcctg cgcgcagcac gctccactgc gcatcgccct gcgccgccac ctgcggcagg
    27361 tgcgtcacgc agagcacctg ccgctccttg ccgagcttgc gcagcgaccg ccccaccgtg
    27421 tccgccactg cgccgccgat gccggcatcc acttcgtcga agaccagggt cgcgacgggc
    27481 gaaacacgcg ccgcgaccag ctgaatcgcc aggctgaggc gcgagagctc gccccctgaa
    27541 gccaccttgg ccaagggccg cagcggcagg ctcgggtgcg acgacacctc gaactcgacg
    27601 gactcttgac cggcggcgct tggcgtctcc agcggctgca gccggacttc gaagcgcccg
    27661 cccttcatcg ccagctgctg catcgacagg gtgaccgctt ccgcaagcgc cttggccgtg
    27721 gccttgcgct tcgccgtcag tttcttcgcc gccgccgcat agcgttcggc cgcggccgcg
    27781 acctccttct gcagcgcttc ggggctcgcc gcgagcgcga gatcggcgag ccgcgtgcgc
    27841 gtcgcgtcgg cgagctccgg cagcgcttcc gcacgcacgc gaaagcgccg gccggccgcg
    27901 tgcagcgcct cgacgcgcgc ttcggcatcg cgcagcgcct gcgggtcgag ctcgacgccg
    27961 gacgcgtagt gtcgcagttc gcgcatcgcc tcgcccgcct gcgcttcggc ggaagacagc
    28021 agctccagga tcggcgcgag ccgggcgtcg tgctcgccca gggcgcgcag ccgggcggcg
    28081 acggcggcaa gctgcggcag gatcgcgccc tcggcttccg aaagggcttc gagcgccgac
    28141 tgcgcgccgg caaggagact cgagccgtgc tcgaggcgtc ggtgctcctc ggccaggcgc
    28201 tcccactcct ggggttgcgg cgcgagcttg tcgagcagca cgcgtctttc ctcgagctcg
    28261 gcgcgctcgg cttcgtgctt cgcgaactgc gcctcggcgt cctcggcaag cgcgcgcagg
    28321 cgctgccagt cccggtaggc ggcggcgcaa tcccgcgcca gcgcctcgcc gccgccgtgc
    28381 gcgtcgagga gctcgcgctg cgcggccggg cgcagcaagg actggtgctc gtgctggccg
    28441 tggatgtcca ccaggtattc gcccgcctcg cgcagctgcg cgagcgtcgc cacgtgcccg
    28501 ttgatgaagc agcgcgagcg cccgccgcgc tccagggtcc ggcgcaggat caggttgccg
    28561 ggatcgccct gcaggtcgcg ctcggcgagc cacgccgcga gcggcgagcg cgcatcgaga
    28621 tcgaactcgg ccgacagctc ggcgcgctcg gcgccttcgc gcaccagcgc gctgtccgcg
    28681 cgcccgccga ccagaagctc gagcgcatcc accaggatcg acttgccggc gccggtctcg
    28741 cccgtgagca cggtatagcc tgggcccggc tccagctcga cgcgctcggc gatgacgtag
    28801 tcgcggaccg acagtacccg cagcatggcg cttcagtcct gctccggccc tctctcggcg
    28861 gcctcgctcc aggacagctt ctcgcgcagc atcgcgaagt agctgtagcc cggcggatgc
    28921 acgaagcgga tggcgtcggc cgagcgcttg agcagcagcc ggtcgccctg caccagctcg
    28981 gtgagggcaa agccgtcgaa atgcgcgcgg gcatcgagcg cacgcagcag cacgatctcg
    29041 atcgcgctgc ggtcggcgac gctcaccggg cgcgccgaga gcacgtgcgg gttgagcggc
    29101 accagcgcga acgcggccac gcccggctgc aggatcgggc cttgggcgga gagcgcgtag
    29161 gccgtggagc ccgtcggcgt ggcgacgatc aggccgtcgg cgcgcagggc gtacacgtac
    29221 tcgccgtcga tgcgcacctc gacctcgatg agccggccct gcgagccctt cccgaccacc
    29281 gcctcgttca gcgccacggt ctgcaggacg cgggtcgcgc cgcgcatgat ctgggcgtcg
    29341 agcagggcgc gctcctcgat gctgtaccgg ccctcgagga tcgccccgac cgaggcgcgc
    29401 atatcctgca gcgcgatgtc ggtcatgaag ccgacccggc cctggttgat gccggcgagc
    29461 ggcaccttgt gccgtgccag gctccgggcc gctgcgagca tggtgccgtc gccgcccagc
    29521 accatggcca ggtcggcgaa cgcgccgatc tcttcatagc ttgccgcgtc ctcgccgtcg
    29581 ccgatgcgcc tggcggtctc gcgctccacg cgcacctcgc agccgcgctc gcgcagcaag
    29641 ccgcgcaggg cgcgcagcga gtcggcgatc tcgggcgtgt gcttgccgat cagcgcgacg
    29701 cgggagaatg ccggcttggg catcgcggca ttctaaccgc atgtgtagaa tgcagcgcat
    29761 gctcgacaag cgcgcgcaga tcctgctgaa gacgctgatt gagcgctaca tcgccgaagg
    29821 gcagccggtc ggctcgcgca ccctgtcccg gcactcgggc ctcgacctgt cgccggccac
    29881 catccgcaac gtgatgtcgg acctggagga gctcggcttc atcgccagcc cgcatacctc
    29941 cgccgggcgc atcccgacgc ccgcgggcta ccgcttcttc gtcgatacgc tgctggtggt
    30001 gaagccgctc ggctcgggcg agcagcgcca gctcgaggac cagctgcatc ccgacaatcc
    30061 gcagcgcgcc atccagtcgg cctcgcagct gctgtcgcag ctgacgcagt tcgccggcgt
    30121 ggtgatgacg ccccggcgcc gcgcgctcgc gttccgccag atcgagttcc tgcgcctgtc
    30181 ggagaagcgc gtgctgctca tcgtcgtgac gccggagggc gacgtgcaga accgggtgct
    30241 attcaccgag cgcgactaca cgcccgccga gctggtctcg gcggcgaact acatcaacca
    30301 gaactacgcc ggcaacaatt tcgaggacgt gcgccatcgc ctgcagcgcg agctgcacga
    30361 gatcaagcag gacatggtgc agctgatgaa cgccgcgctc gaggccggca accgcgcgct
    30421 cgaggaaggc aatgcgcctt acgtcatctc gggcgagcgc aacctcctga ccgcgcagga
    30481 cctgtcgcac gacctgcggc gcctgcggca gctgttcgag ctgttcgagc acaagacgac
    30541 gctgctgcag atcctggacc tgtcgctgcg cggccagggc gtgcaggtgt tcatcggcgg
    30601 cgagtccggc gtcagcgcgc ccgacgacgt gagcgtggtc accgcgccct acaaggtcga
    30661 cggcgaggtg gtcggcaccg tcggcgtcat cggcccgtcg cgcatggcat acgaccgcgt
    30721 cgtgcccgtc gtcgacgtta cggcgaagct cctgtcgagc gcgctgtcca agccctgagc
    30781 ggcatcctcc tcgtcaatct gggcacgccc gcggcgccga ccccggcggc ggtgcgcgct
    30841 tatctggccg agtttctctc tgacccgcgc gtggtcgagc tgccgcgctg gctgtggctg
    30901 ccgatcctgc acggcattgt cctgcgcaag cggccggccg catcggccag gaagtacgcg
    30961 aaggtgtgga ccgcggaagg ctcgcctctc gcggtgcata ccgcgcgcca ggccgcactg
    31021 ctgcgcgcgg ccactggcct gcccgtggaa tacgccatgc gctacggcga gccgagcatc
    31081 gccgcggcgc tgcgcaagct gccccctggg ccgcgcatca ttccgctgta cccgcagtac
    31141 gcgcgcagca cgaccgagtc gatccgcgac gtggtcagcc cggacgcgga gttcgtcgag
    31201 cagttccacg accacccggg ctacatcgcc gcgctcgccg cgctcgtcga ggagcgcccc
    31261 gacgtgctgg tgatgagctt tcacggcctg ccccagcgct cggtggagcg cggcgacccc
    31321 tatcaggcgc agtgcctgcg caccgcgcgg ctgctggccg atgccctgcg gctgccgccc
    31381 gaccggtacc gcgtcacctt ccagtcgcgc ttcggcccgg ccaagtggct gcagccctac
    31441 acctcggagg tgctcgccga actcggcgcc gcgcgcacgc cacgcgtgca cgtgatctgt
    31501 ccgggcttcg tgtccgactg cctggagacg ctcgaggaga tcgcgctcga gggccgcgac
    31561 atcttcctgc acgcgggggg cggcgagttc aaggcactgc cctgcctgaa cgagtcgccg
    31621 cgctggatcg aggcgctcgc cgcgatcgct acttcttctt ctcgggcc
//
LOCUS       DBNHKE010000015         7780 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_796136, whole genome shotgun sequence.
ACCESSION   DBNHKE010000015 DBNHKE010000000
VERSION     DBNHKE010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_796136"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..560)
                     /gene="mlaD"
                     /locus_tag="AC16O9_01030"
     CDS             complement(81..560)
                     /gene="mlaD"
                     /locus_tag="AC16O9_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR6515233.1"
                     /translation="MTRSAIDLWVGVFVAIGLGALLFLALKVGNLASFSTAETYRVEA
                     RFSNIGGLKVRAPVKSAGVVVGRVADIGFDSKSYEAVAIFTIDSRYRFPRDTSAKILT
                     SGLLGEQYIGLEAGADERTLKDGERLRLTQSAVVLENLIGQFLFNKAAEGGDAKDGK"
     gene            complement(562..1317)
                     /gene="mlaE"
                     /locus_tag="AC16O9_01035"
     CDS             complement(562..1317)
                     /gene="mlaE"
                     /locus_tag="AC16O9_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIR6515234.1"
                     /translation="MVTTGVWRLGYASRFFLYLLAHSGTAFRRFRLTVREVYFAGVLS
                     LIIILVSGLFVGMVLGLQGYDTLQRYGSSEALGVLVALSLVRELGPVVAGLLFASRAG
                     SAITAEIGLMKATEQLSAMEMMAVDPIARVVAPRFWGGVLSMPLLAALFSAMGIFGGY
                     LVGVVLIGVDEGAFWSQMQAAVDVRNDILNGVIKSVVFGVAVTLIAVFEGYDAVPTAE
                     GVSGATTRTVVTSSLAILALDFVLTALMFTGGA"
     gene            complement(1350..2162)
                     /locus_tag="AC16O9_01040"
     CDS             complement(1350..2162)
                     /locus_tag="AC16O9_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515235.1"
                     /translation="MADPAAIAQIEDLTFAYGQAPVLHGIDLEIPRGKVVAIMGQSGC
                     GKTTLLRIIAAALLPTRGRARVLGQVTGELDRDGLYALRRRMGMLYQFGALFTDMTVF
                     DNVAFQMREHTDLPEDAIRDLVLLKLEAVGLRGAAPLMPAELSGGMARRVALARAIAL
                     DPELILYDEPFTGLDPIALGVIARLVRQLNDALGATAVIVTHDVAETLPLVDYVYFMA
                     DGRVVAQGTPAEVRRSGVPFVEQFVSGATDGPVPFHYPAAGYSADLGLAGSA"
     gene            2243..2977
                     /locus_tag="AC16O9_01045"
     CDS             2243..2977
                     /locus_tag="AC16O9_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515236.1"
                     /translation="MDRVVANAAIRVAGLVKRFGKREALAGVDLEVAPGEAFGLVGAN
                     GAGKTTLLKCLLDLCAFDAGRIEIFGAPSNEARARARLAYVPERFLPPHYLRGREFLE
                     LTVRLGGVAYEEAAARALLAELALDAEVLERPVRELSKGMTQKLGLAGGFLLARELTV
                     LDEPMSGLDPASRLAVKSVLARLHAEGRTLLFTSHVLADVEELCTTIAVLDRGRLCFR
                     GAPAALTARYGESSLERAFLRCVNGD"
     gene            2967..4394
                     /locus_tag="AC16O9_01050"
     CDS             2967..4394
                     /locus_tag="AC16O9_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6515237.1"
                     /translation="MAIETHDARGGKPQLLIVDDDTLIAETLTFALGVDFEVYACESR
                     AHAVELLRQLPQPPQLALVDLGLPPRPHAPDEGFQLIRDLLAHSPGMRIFVLSGQGDA
                     AHARHARALGAVEFIAKPCDPALLKAALLRALAFRPAEPAGPPGQAARLIGASAALVR
                     LRSQITQCADSPFPVLIEGESGSGKEQVALALHQLSQRAAKPYLALNCAAIAPTLVEP
                     TLFGYVKGAFTGATANKSGYFEDAQDGTLFLDEIGELPPEIQAKLLRVLENGEYQRVG
                     ETQGRVARARVIAATNRDLRQEMRQGRFRTDLYHRLSVLTVAVPPLREMDRDRLLLLE
                     HFRAQYSAQAGVPPFSLDADAERAWLAYAFPGNVRELRNIVVRLTTKYAGRRVSAAEL
                     EPEFDLATAAGDAPPVPDLLAEAQRELERGGFSLDETLKARERAYVEAALRMTRGNVS
                     QAAKLLGVNRTTLYSRMAPAEDKER"
     gene            4398..5780
                     /locus_tag="AC16O9_01055"
     CDS             4398..5780
                     /locus_tag="AC16O9_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR6515238.1"
                     /translation="MSLYLEHFGLKEAPFRITPHTDFFFDGADRGATLEALLYATLHE
                     EGIVKVVGEVGSGKTMLCRVLIERLPAHVETVYLANPSFTRDEILHAVAEELDIEVPR
                     ERVTGALNALHDRLIESYGGGRRTVILIDEAHAMPEETLEQVRLLSNLETSRHKLLQI
                     VLFGQPELDEVLALASMRQLKDRITHSFRMRPLQAAEVGTYLSFRMRAAGYRGPDIFA
                     PAAVTLIARAAGGLSRRINILADKSLLSAYSEGAHGVGVRQARAAIADSEFAPRWRAP
                     RGRLAFAAAAAAGVLLGAGAYWLLAPAPVPPRVQAPASAPVAPPQAAAPSAPAPQPQP
                     MLEREQMLRLAGYDAGRNRLLHARIEAARDKLDSAPDARHSIELYFTDNSDPDRVERF
                     LTRARDLVPLENLYIVPVESGGRYRIWALYGDFAGEPEALQAAKRLPPKYQQAFPLVP
                     RSFAEVRRAL"
     gene            5890..7563
                     /locus_tag="AC16O9_01060"
     CDS             5890..7563
                     /locus_tag="AC16O9_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HIR6515239.1"
                     /translation="MTWKLTVTVVALASALAGCAQAPAKPSETHLGVEAPPPITPGTI
                     PPPVQIAPVLPEPRPVAPPETYSVVVSNVSAPDLLFALARDAKLNIDIHPGISGNVTL
                     NAVDQTLPQLLERISRQISMRYELNGANLLVMPDAPFLRTYLIDSLSASRNVKMQSQA
                     STQFGAASASGGGTTGATATIDVTSNNQLWDTLVQNIKDILGEASGAAPAAGAAAATG
                     AGGGATGVIGNRESGVLYVRANSRQHERVREFLDSVLANVRRQVLIEATVAEVQLNNE
                     YQRGIDWQRLRTGAGATGTAAFGTGQSGVEFSQVASGTPAGITTQAFVLGGALVSQNL
                     NVAIRLLESFGNVRVLSSPKISVLNNQTALLRVTRDIVYFTVTPSQQTGAAAGGGTVV
                     LPATFTTAPVVAAEGFVMSVLPQINDVEGIMLNVRPTIRRRVTSAFDPNPALAAAGTV
                     NEIPVFETREFDSMLRIQSGQTAVLGGLMQDSVENVEDTIPGLNRIPFFGKLFAQERK
                     VTRKTELVIFLRATVIRDPSVDGDYARYRNLLPTENFLTRPAGSAPAPQ"
     gene            7565..>7780
                     /locus_tag="AC16O9_01065"
     CDS             7565..>7780
                     /locus_tag="AC16O9_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515240.1"
                     /translation="MSLLLEALKRAEKAKEEAQRRSREGGGASELRLAEPAAADAQKP
                     VVTRDELPPITSPLEIQSEDLRVEAPAT"
BASE COUNT         1234 a         2785 c         2670 g         1091 t
ORIGIN      
        1 cggatcgcgc gcatcgccgc cgcccgtggt ggcgcagccg ccggcggcga gcgccagcgc
       61 gagaacgagc gtccgagcgc tcacttgccg tccttcgcgt cgccgccctc ggcggccttg
      121 ttgaacagaa actggccgat caggttttcc agcaccaccg cggactgcgt gaggcgcagg
      181 cgctcgccat ccttcagcgt gcgctcgtcg gcaccggctt cgaggccaat gtactgctcg
      241 ccgagcagcc ccgaggtgag gatcttggcc gaggtgtcgc gagggaagcg gtagcgcgaa
      301 tcgatcgtga agatcgccac cgcctcatag ctcttgctgt cgaagccgat gtcggccacg
      361 cgcccgacca ccacgccggc gctctttacc ggcgcgcgca ccttcagtcc gccgatgttg
      421 gaaaagcgcg cttccacccg gtaggtctcg gcggtggaga aggaagcgag gttgccgacc
      481 ttcagcgcga ggaacaagag cgccccgagg ccgatcgcga cgaagacgcc gacccacagg
      541 tcgatggcgg aacgagtcat ttcaggcacc tccggtgaac atgagcgcgg tgagcacgaa
      601 atcgagcgcg agtatggcga gcgaggacgt caccacggtg cgcgtggtcg ctccggaaac
      661 cccctcggcg gtgggcaccg cgtcgtagcc ctcgaacacc gcgatcaatg tcacggcgac
      721 gccgaacacg acgctcttga tgacgccgtt caggatgtcg ttgcgcacgt ccaccgccgc
      781 ctgcatctgc gaccagaacg cgccttcgtc cacgccgatc agcaccaccc ccaccaggta
      841 gccgccgaag atgcccatcg ccgagaacag cgccgcgagc agcggcatcg acagcacgcc
      901 gccccagaag cgcggcgcga ccacgcgcgc gatcgggtcc accgccatca tctccatcgc
      961 cgagagctgt tcggtggcct tcatcaggcc gatctcggcg gtgatcgcgc tgccggcgcg
     1021 gctcgcgaag agcagcccgg cgaccaccgg cccgagctcc cgcaccagcg acagcgccac
     1081 cagcaccccg agcgcctccg aggagccgta gcgctgcagc gtgtcgtagc cctgcaggcc
     1141 gagcaccatg ccgacgaaca agccgctcac cagaatgatg atgagcgaga gcacgcccgc
     1201 gaagtagacc tcgcgcaccg tgagccggaa acggcggaac gcggtccccg agtgggcaag
     1261 caggtacaga aagaagcgac tggcgtagcc caggcgccat acgccggtcg tcaccatggc
     1321 accgagtcgg acgagggcgt ggcgcaaagc taggcgctgc cggcgaggcc gaggtcggcg
     1381 gagtagccgg cggccggata atgaaaaggc accggcccgt cggtcgcgcc actgacgaac
     1441 tgctcgacga agggcacgcc ggagcggcgc acctcggcgg gggtgccctg ggcgacgacg
     1501 cgcccgtcgg ccatgaagta gacgtaatcc acgagcggca gggtctcggc aacgtcgtgc
     1561 gtgacgatga ccgcggtggc gccaagcgcg tcgttcagct gccgcaccaa acgggcgatg
     1621 acgccgagcg cgatcgggtc gagcccggtg aacggctcgt cgtacaggat cagctccgga
     1681 tcgagcgcga tggcgcgcgc cagcgcgacg cgccgcgcca tgccgcccga gagctccgcc
     1741 ggcatgagcg gcgccgcgcc gcgcagtccc accgcctcga gcttgagcag caccaggtcg
     1801 cggatcgcgt cctccggcag gtcggtgtgc tcgcgcatct ggaacgccac gttgtcgaac
     1861 accgtcatgt cggtgaacag cgcgccgaac tggtacagca tgcccatgcg ccggcgcagt
     1921 gcgtacaggc cgtcgcggtc gagctcgccg gtcacctggc cgagcacgcg cgcccggcct
     1981 cgcgtgggca gcagcgccgc cgcgatgatc cgcagcagcg tcgtcttgcc gcagcccgac
     2041 tggcccatga tcgccaccac cttgccgcgc gggatctcga ggtcgatgcc gtgcagcacc
     2101 ggcgcttgcc cgtaggcgaa cgtcaagtct tcgatctgtg cgatggcggc agggtcagcc
     2161 acggggtggg gcggggacgg cggcaatgcg ctgattttac tcgacgcgct ggggcaagcc
     2221 ggtttcacct atagttaggg gcgtggacag ggtagtcgcc aacgccgcga tccgcgtggc
     2281 gggtctggtc aaacgcttcg gcaagcgcga ggcgctggcc ggcgtcgacc tggaggtggc
     2341 gccgggcgag gccttcggcc tggtcggggc caacggcgcg ggcaagacga cgctgctgaa
     2401 atgcctgctc gatctgtgcg ccttcgatgc cggccgcatc gagatcttcg gcgcgccgag
     2461 caacgaggcg cgcgcacgcg cgcgcctcgc ctacgttccc gagcgatttc tgccgccgca
     2521 ctacctgcgc gggcgcgagt tcctggagct gaccgtgcgc ctcggcggag tcgcttatga
     2581 ggaagccgcg gcgcgtgcgc ttctcgccga gctcgcgctg gacgccgagg tgctcgagcg
     2641 cccggtgcgc gagctctcca agggcatgac gcaaaagctc ggtctcgccg gggggttcct
     2701 gctcgcgcgc gagctcacgg tgctcgacga gccgatgagc ggcctcgacc cggcaagccg
     2761 cctcgcggtg aaatccgtcc tcgcgcgcct gcatgccgaa gggcgcacgc tgctgttcac
     2821 ctcgcacgtg ctggccgacg tggaggagct gtgcacgacc atcgccgtgc tcgaccgcgg
     2881 ccgcctctgc tttcgcggcg cgccggcggc gctcaccgcg cgctacggcg aatcgagcct
     2941 ggagcgcgct ttcctgcgtt gcgtaaatgg cgattgagac gcacgatgcg cgcggcggca
     3001 agccgcagct gctgatcgtc gacgacgaca cgctgatcgc cgagaccctg accttcgccc
     3061 tcggcgtcga tttcgaggtc tacgcctgcg aatcgcgcgc ccatgccgtc gagctgctgc
     3121 gccagctgcc gcagccgccg cagctggcgc tcgtcgacct cgggctgccg ccgcgcccgc
     3181 acgcccccga tgagggcttc cagctgatcc gcgatctgct cgcacactcg cccggcatgc
     3241 gcatcttcgt gctctccggc cagggcgacg cggcgcacgc gcgccacgcg cgggcgctgg
     3301 gcgccgtcga gttcatcgcc aagccctgcg atcccgcgct gctcaaggcg gcgctgctgc
     3361 gcgcgctcgc gttccggccc gccgagccgg cgggcccgcc gggccaggcc gcgcgcctca
     3421 tcggcgcaag cgcggcgctc gtgcgcctgc gcagccagat cacgcaatgc gcggactcgc
     3481 ccttcccggt gctgatcgaa ggcgaatccg gcagcggcaa ggagcaggtg gcgctggcgc
     3541 tgcaccagct ctcgcagcgc gccgccaagc cctacctggc gctcaactgc gcggcgatcg
     3601 cgccgacgct ggtcgagccg acgctgttcg gctatgtgaa gggggcgttt accggcgcca
     3661 ccgccaacaa gtcgggctac ttcgaggacg cgcaggacgg gactctgttt ctcgacgaga
     3721 tcggcgagct gccgccggag atccaggcca agctgctgcg cgtgctcgag aacggcgagt
     3781 accagcgcgt cggcgagacg cagggacgcg tggcgcgcgc gcgcgtcatc gccgccacca
     3841 accgcgacct gcgccaggag atgcgccagg gtcgcttccg caccgacctg taccaccgcc
     3901 tgtccgtgct caccgtggcg gtgccgccgc tgcgcgagat ggaccgcgac cggctgctgc
     3961 tgctcgagca tttccgcgcc cagtactcgg cgcaggcggg cgtcccgcct ttctcgctcg
     4021 atgccgacgc cgagcgcgcc tggctcgcgt atgcctttcc gggcaacgtg cgcgagctgc
     4081 gcaacatcgt cgtccggctc accaccaagt acgccggcag gcgcgtttcg gccgccgagc
     4141 tggagcccga attcgacctc gcaaccgccg ccggggacgc gccgccggtg cccgacctgc
     4201 tcgccgaagc ccagcgcgag ctggagcgcg gcggcttcag cctcgacgag accctgaagg
     4261 cgcgcgagcg cgcctacgtc gaggccgcgc tcaggatgac gcgcggcaac gtcagtcagg
     4321 cggcgaagct gctgggagtg aaccggacga cgctctacag ccggatggcc cccgccgagg
     4381 acaaggagcg ctaggcgatg tcactgtacc tcgagcactt cggcctcaag gaggcgccct
     4441 tccgcatcac gccgcatacc gacttcttct tcgacggcgc cgaccgcggc gccacgctcg
     4501 aggcgctgct ctatgccact ttgcacgagg aaggcatcgt caaggtggtg ggcgaagtgg
     4561 gcagcggcaa aacgatgttg tgccgggtgc tgatcgagcg gctgccggcg cacgtggaga
     4621 ccgtgtacct cgccaatccc tcgttcacgc gcgacgagat cctccacgcg gtggccgagg
     4681 agctcgacat cgaggtcccg cgcgagcgcg tcaccggcgc gctgaacgcg ctgcacgacc
     4741 ggctgatcga gtcctacggc gggggacggc gcaccgtgat cctgatcgac gaggcgcacg
     4801 ccatgcccga ggagacgctc gagcaggtgc ggctgctatc gaacctggaa acgagccgcc
     4861 acaagctgct gcagatcgtg ctgttcggcc agcccgagct ggatgaggtg ctggcgctcg
     4921 cctcgatgcg ccagctgaag gatcgcatca cgcacagctt tcgcatgcgc ccgctgcagg
     4981 cggcggaagt cggcacgtac ctgtcgttcc ggatgcgcgc cgccggctac cgcggccccg
     5041 acatcttcgc cccggcggcc gtgacgctga tcgcacgggc cgccggcggg ctttcccggc
     5101 gcatcaacat cctcgccgac aagtcgctgc tctcggccta cagcgaaggc gcccacggtg
     5161 tgggcgtcag gcaagcgcgc gccgccatcg ccgactccga gttcgcgccg cgctggcgcg
     5221 cgccgcgagg gcggctcgct tttgccgccg cggccgccgc gggcgtgctc ctgggagcgg
     5281 gcgcctattg gctgcttgcg cctgccccgg tgcccccgcg cgtgcaggcc ccggcatcgg
     5341 cgcccgtcgc gccaccgcag gctgcggcgc cgagcgcgcc ggcgccgcaa ccgcaaccca
     5401 tgctcgagcg cgagcagatg ctgcgcctcg ccggctacga cgccgggcgc aaccggctgc
     5461 tgcacgcgcg gatcgaagcc gcccgcgaca agctcgattc ggcgcccgac gcccgccaca
     5521 gcatcgagct gtacttcacc gacaacagcg accccgaccg ggtggagcgg ttcctgacgc
     5581 gggcgcgcga cctggtgccg ctcgagaacc tgtacatcgt gccggtggaa agcggcggcc
     5641 gctaccggat ctgggccttg tatggggact tcgccggaga gcccgaggca ctgcaggccg
     5701 ccaagcgcct gccgcccaaa taccagcagg cctttcccct ggtgccgcga agctttgccg
     5761 aagtccgccg cgcgctctag gcaggggtat ctgcaagatc ctgaaaactg tcccacgaca
     5821 gtcgcttagc ggcttttctt caggcacgct cgtgctatgc tagctatccg agggaggcgc
     5881 tcagacctga tgacgtggaa gctaacggtg accgtggttg cgctcgcgtc ggcactggcg
     5941 ggctgcgcgc aggcgcccgc gaagccgtcg gagacccatc tgggcgtcga ggcgccgccg
     6001 cccatcacgc ccggcaccat cccgccgccg gtgcagatcg cccccgtact gccggagccc
     6061 aggccggtgg ccccgcccga gacctacagc gtggtcgtca gcaacgtcag cgcgccggac
     6121 ctgctgttcg cgctggcccg cgacgccaag ctgaacatcg acattcaccc gggcatttcc
     6181 gggaacgtga cgctgaatgc ggtggaccag acgctgccgc agcttctcga gcgcatctcg
     6241 cgtcagatca gcatgcgcta cgagctgaat ggcgccaatt tgctggtcat gcccgatgcg
     6301 ccgttcctgc gcacctacct gatcgactcg ttgagcgcgt cgcgcaacgt gaagatgcag
     6361 tcgcaggcat ccacccagtt cggcgccgcg agcgcaagcg ggggaggcac cacgggcgcc
     6421 acggcgacca ttgacgtcac gtccaacaat cagttgtggg acacgctggt gcagaacatc
     6481 aaggacatcc tcggcgaggc ttcgggcgcc gcgccggccg cgggcgccgc cgccgcaacg
     6541 ggcgccggcg gcggggccac cggcgtcatc ggcaaccgcg agagcggcgt gctctacgtg
     6601 cgcgccaact cgcgccagca cgagcgcgtg cgcgagttcc tcgattcggt gctggccaac
     6661 gtgcggcggc aggtcctgat cgaagccacg gtcgccgagg tgcagctgaa caacgagtac
     6721 cagcgcggca tcgactggca gcgcctgcgc acgggtgccg gcgccaccgg caccgccgcc
     6781 tttggcaccg gacagtcggg cgtggagttc agccaagtcg ccagcggcac gccggcggga
     6841 atcacgaccc aggcgttcgt gctcggcggg gcgctggtca gccagaacct gaacgtcgcc
     6901 atccggttgc tggagtcttt cggcaatgtg cgggtgctct cgagcccgaa gatcagcgtc
     6961 ctcaacaatc agacggcgct cctgcgcgtt acccgcgaca tcgtctattt cacggtcacg
     7021 ccctcccagc agacgggcgc cgctgccggg ggcggaaccg tggtcctgcc tgcgactttc
     7081 acgacggcgc ccgtggtcgc cgccgagggt ttcgtcatga gcgtgctgcc gcagatcaac
     7141 gacgtcgagg gcatcatgct caatgtgcgc ccgacgatcc ggcgcagggt gacctccgcc
     7201 ttcgatccca accctgcgct ggccgcggcc ggaaccgtga acgaaatccc ggtgttcgag
     7261 acgcgcgagt tcgattcgat gctgcgtatc cagagcggcc agaccgcggt gctcggcggc
     7321 ctgatgcagg actcggtgga gaacgtcgag gacaccattc cgggcctgaa ccgcatcccc
     7381 ttcttcggca agctgttcgc ccaggagcgc aaggtgacgc gcaagacgga actggtcatc
     7441 ttcctgcgcg ccacggtgat ccgcgatccg agcgtcgacg gcgactacgc ccgctaccgc
     7501 aacctgctgc cgacggagaa cttcctcacc cggccggcgg gcagcgctcc cgcgccgcag
     7561 tgacatgagc ctgctgctcg aggcgctcaa gagggccgag aaggccaagg aggaggccca
     7621 gcgccgctcc cgcgaaggtg ggggcgcaag cgagctgcgg ctggcggaac cggcggcggc
     7681 ggacgcgcaa aagccggtgg tgacgcgcga tgagctgccg ccgatcacca gcccgctcga
     7741 gatccagtcg gaggacctgc gcgtcgaggc gccggccacc
//
LOCUS       DBNHKE010000016         4461 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1272299, whole genome shotgun sequence.
ACCESSION   DBNHKE010000016 DBNHKE010000000
VERSION     DBNHKE010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1272299"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /gene="rpe"
                     /locus_tag="AC16O9_01070"
     CDS             <1..515
                     /gene="rpe"
                     /locus_tag="AC16O9_01070"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6515241.1"
                     /translation="KPHVRLPIDVHLMVKPVDRIIPEFARAGASIISFHPEATEHVDR
                     TVGLIKELGCRAGLVLNPATPLGCLDHTLDKLDLVLLMSVNPGFGGQQFIRSVLPKIA
                     EVRRRIERLGREVWLEVDGGVKTDNIAEIAAAGADTFVAGSAIFGAKDYAATIREMRR
                     RVEAGIVPTP"
     gene            654..2144
                     /gene="trpE"
                     /locus_tag="AC16O9_01075"
     CDS             654..2144
                     /gene="trpE"
                     /locus_tag="AC16O9_01075"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR6515242.1"
                     /translation="MTESEFERLAQQGYTRIPVLAEARADLYTPLAVYLKLAGGPHSY
                     LLESVVGGERFGRYSFIGLPCAERIEARGASVRRLLRDARGADVCLEEALGDPFEFVR
                     AYLRRHRVAPAPGALKFPGGLAGYFGYECVRHVEPRALGAAKPDALGTPDMLLLVSDE
                     LAVVDNVMGKLHLVVYADPRQSGAWRAASERLERLRARLAAPLPEHMVLAQPREEGAS
                     APLSRSVTEAQFLAAVQRTKEYIFAGDCMQAQISQRTSRAFGAPPISLYRALRSLNPS
                     PYMFYFDFGDHHVIGASPEIMVRVAGDRITLRPIAGTRPRGATPEDDTRIAAELLADP
                     KERAEHVMLIDLGRNDVGRVAATGSVQVTEKMVVERYSHVMHIVSNVEGRCAPGVDAL
                     DVFKASFPAGTVTGAPKVRAMQIIDELEPVKRGVYSGAVGYLGFNGDMDVAIAIRTAV
                     VKDGQLHVQAAAGVVADSDPQSEWQETQHKARAILRAAEVATAEAP"
     gene            2141..2713
                     /locus_tag="AC16O9_01080"
     CDS             2141..2713
                     /locus_tag="AC16O9_01080"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR6515243.1"
                     /translation="MILVLDNYDSFTYNLVQYLGELGAEVKVARNDELSVDEIEALAP
                     ERVVISPGPCTPNEAGISLALVARLAGKVPILGVCLGHQAIGQAFGGKVVRARQVMHG
                     KVSRIRHDGRGVFAGVPDEFVATRYHSLVVERATLPAVLEVTAQAEDGEIMGLRHAAL
                     AVEGVQFHPEALLTEHGHRMLENFLRGDSR"
     gene            2710..3750
                     /gene="trpD"
                     /locus_tag="AC16O9_01085"
     CDS             2710..3750
                     /gene="trpD"
                     /locus_tag="AC16O9_01085"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR6515244.1"
                     /translation="MKQITVAEAIQRTVEHREVFHDEMLHVMRQIMRGELTPAQIAGF
                     VVGLRVKKETIGEIAAAAQVMREFATPVKVSDATHLVDTCGTGGDAAHTFNVSTASAF
                     VAAAAGARVAKHMGRSVSSASGSAEVLEALGANIALTPEQTGQAINALGLGFMFAPAH
                     HAAMKHAAPVRKDLGVRTIFNILGPLTNPAGAANQVMGVFHPDLVGIQVRVLQRLGSK
                     HVMTVYGLEGLDEISISGETMVGELVNGEIREYTMHPQQFGIELYDRRAIQVNTVDES
                     RQMILAVLEGQPGPALNIVLLNAGAAIYVAGVADSMQAGIDRARRAVAKGEARAKLDE
                     FVAFTKQVAAKA"
     gene            3755..>4461
                     /gene="trpC"
                     /locus_tag="AC16O9_01090"
     CDS             3755..>4461
                     /gene="trpC"
                     /locus_tag="AC16O9_01090"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR6515245.1"
                     /translation="MADVLQRILETKRAEVRAAKAALPPAEIERRARAAPPARDFVGA
                     LRAKIAAGRPAVIAESKRASPSKGLLRDPYDPAAIARSYERGGAACLSVLTDREYFQG
                     APEHLAAARAACALPVLRKDFMLDPYQVVEARAMGADCILLIAAAIDLPLMRELEDAA
                     LALGMAVLVEVHDGAELDRALRLRTPLVGINNRDLRTFETRLETTLRLHARVSAPRLV
                     VSESGIAARGDVSKLWQA"
BASE COUNT          662 a         1558 c         1609 g          632 t
ORIGIN      
        1 tgaagcccca tgtgcgcctg ccgatcgacg tgcacctgat ggtgaagccc gtggatcgca
       61 tcattcccga gttcgccagg gccggcgcga gcatcatcag cttccatccc gaggccaccg
      121 agcacgtcga tcgcaccgtc ggcctgatca aggaactcgg ctgccgcgcg gggctggtcc
      181 tcaacccggc gacgccgctc ggctgcctcg accacacgct ggacaagctc gacctggtgc
      241 tgctcatgtc ggtcaacccg ggcttcggcg gccagcagtt cattcgcagc gtgctgccga
      301 agattgccga ggtgcgccgg cgcatcgagc gcctcgggcg cgaggtctgg ctggaagtcg
      361 acggcggcgt gaagaccgac aacattgcgg agatcgcggc cgcgggggcg gacaccttcg
      421 tcgccgggtc cgccatcttc ggcgccaagg actacgccgc gaccatccgc gagatgcgcc
      481 ggcgcgtcga ggctggtata gttcccacgc cgtgacccac gtgcgctctc tccgctggca
      541 ggtctggcgc ccctggcgcc gctagctccc cacgcgtccc gcgcggctcg gcgacgcgtc
      601 acccccgagg ccgcacggca gtccccttga agtaagttgc agcggagaga ttcatgaccg
      661 agtccgagtt cgagcggctc gcgcaacaag gctatacccg cattccggtg cttgccgagg
      721 cgcgcgccga cctctacacg ccgctcgcgg tgtacctgaa gctcgccggc gggccgcaca
      781 gctacctgct ggagtccgtc gtcggcggcg agcgcttcgg gcgctactcg ttcatcggcc
      841 tgccctgtgc cgagcgcatc gaggcgcgcg gcgcctcggt gcggcgcctg ctgcgcgatg
      901 cgcgcggtgc cgacgtgtgc ctcgaggagg cgctgggcga tccgttcgag ttcgtgcgcg
      961 cctacctcag gcgccaccgc gtggcgccgg cgcccggggc gctgaagttc cccggcggcc
     1021 tggccggcta tttcgggtac gagtgcgtgc gccacgtgga gccgcgcgcg cttggcgccg
     1081 ccaagcccga tgcgctgggc acgcccgaca tgctgctgct ggtgtccgac gagctggcgg
     1141 tggtggacaa cgtgatgggc aagctgcatc tcgtcgtcta tgccgacccg cggcaatccg
     1201 gcgcctggcg cgcggcaagc gagcggctcg agcgcctgcg cgcgcggctc gccgcgccgc
     1261 tgcccgagca catggtgctc gcgcagccgc gtgaagaagg cgcgagcgcg ccgctcagcc
     1321 gctcggtgac cgaggcgcag tttctcgccg ccgtgcagcg cacgaaggag tacatcttcg
     1381 ccggcgactg catgcaggcg cagatctcgc agcgcacctc gcgcgccttc ggcgcgccgc
     1441 cgatcagcct gtaccgcgcg ctgcgcagcc tcaatccctc gccctacatg ttctacttcg
     1501 atttcggcga ccaccacgtg atcggcgcct cgcccgagat catggtgcgc gtcgccgggg
     1561 accggatcac cctgcgcccg atcgccggca cgcggccgcg cggcgcgacg cccgaggacg
     1621 acacgcgcat cgccgcggag ctgctcgccg acccgaagga gcgcgccgag cacgtgatgc
     1681 tgatcgatct gggcaggaac gacgtcggcc gcgtcgccgc cacgggcagc gtgcaggtga
     1741 ccgagaagat ggtggtggag cgctattcgc acgtgatgca catcgtctcc aacgtcgaag
     1801 gacgctgcgc gccgggggtg gacgcgctcg acgtgttcaa ggcgagcttt ccggcgggca
     1861 ccgtcaccgg tgcgcccaag gtgcgcgcga tgcagatcat cgacgagctc gagcccgtga
     1921 agcgcggcgt ctatagcggc gcggtcggct acctcggctt caacggcgac atggacgtgg
     1981 cgatcgccat ccgcaccgcg gtggtgaagg acgggcagct gcacgtgcag gcggccgcgg
     2041 gcgtggtggc cgactccgat ccgcagtccg agtggcagga gacgcagcac aaggcgcgcg
     2101 ccatcctgcg cgcggccgag gtggccacgg cggaggcacc gtgatcctgg tgctcgacaa
     2161 ttacgattcg ttcacctaca acctcgtgca gtacctgggc gagctcggcg ccgaggtgaa
     2221 ggtggcgcgc aacgacgagc tgagcgtcga cgagatcgag gcgctcgcgc ccgagcgcgt
     2281 ggtcatctcg ccgggcccgt gcacgccgaa cgaggcgggc atctcgctcg cgctcgtcgc
     2341 gcgcctggcg ggcaaggtgc cgatcctcgg ggtgtgcctc ggccaccagg ccatcggcca
     2401 ggccttcggc ggcaaggtgg tgcgcgcgcg gcaggtgatg cacggcaagg tgtcgcgcat
     2461 ccgccacgac ggccgcggcg tgttcgcggg cgtacccgac gagttcgtcg ccacgcgcta
     2521 tcactcgctg gtggtggagc gcgcaacgct gcccgcggtg ctcgaagtga cggcgcaggc
     2581 cgaagacggc gagatcatgg gcttgcgcca tgccgcgctg gccgtcgagg gcgtgcagtt
     2641 ccatccggag gcgctgctca ccgagcacgg ccaccggatg ctcgagaatt tcctcagggg
     2701 agacagccga tgaaacagat cacggtcgcc gaggcgatcc agagaacggt ggagcaccgc
     2761 gaggtgttcc acgacgagat gctgcacgtg atgcgccaga tcatgcgcgg cgagctcacg
     2821 ccggcgcaga tcgcgggctt cgtcgtcggg ctgcgcgtga agaaggagac catcggcgag
     2881 atcgccgcgg cggcgcaggt gatgcgcgag ttcgcgacgc cggtcaaggt gagcgacgcg
     2941 acgcacctgg tcgacacctg cggcaccggc ggcgacgccg cgcacacctt caacgtgtcc
     3001 acggcctcgg cgttcgtcgc cgcggcggcc ggggcgcgcg tcgccaagca catggggcgc
     3061 tcggtgtcct cggcctcggg cagcgcggag gtgctggagg cgctcggcgc gaacatcgcg
     3121 ctgacgccgg agcagaccgg gcaggcgatc aacgcgctcg gcctggggtt catgttcgcc
     3181 ccggcgcacc acgcggcgat gaagcacgcc gcgccggtga gaaaggacct cggcgtgcgc
     3241 acgatcttca acatcctcgg gccgctcacc aatcccgcgg gcgcggccaa ccaggtgatg
     3301 ggcgtgttcc accccgacct ggtcggcatc caggtgcgcg tgctgcagcg cctgggctcc
     3361 aagcacgtga tgacggtcta cgggctggag ggcctggacg agatctcgat ctccggagaa
     3421 accatggtcg gcgagctggt gaacggcgag atccgggagt acacgatgca tccgcagcag
     3481 ttcggcatcg agctctacga ccggcgcgcc atccaggtca acaccgtgga cgagtccagg
     3541 cagatgatcc tcgcggtgct cgaggggcag cccgggccgg ccctcaacat cgtgctgctc
     3601 aacgccggcg cggcgatcta cgtggcgggc gtcgccgact cgatgcaagc gggcatcgac
     3661 cgcgcgcggc gcgccgtcgc caagggcgag gcgcgtgcga agctcgacga gttcgtggcg
     3721 ttcacgaagc aggtggcggc gaaggcctga gcgcatggcc gacgtcctgc agcgcatcct
     3781 cgagaccaag cgcgcggagg ttcgcgccgc caaggccgcg ctgccgcccg ccgagatcga
     3841 gcgccgcgcg cgcgcggcgc cgccggcgcg cgatttcgtc ggcgcgctgc gcgcgaagat
     3901 cgccgccggc cggcccgcgg tgatcgccga atcgaagcgc gcgagcccgt ccaagggcct
     3961 gctgcgcgat ccctacgacc cggccgcgat cgcgcgcagc tacgagcgcg gcggcgcggc
     4021 ctgcctgtcg gtgctcaccg accgcgagta cttccagggc gcgccggaac accttgccgc
     4081 ggcgcgcgcc gcatgcgcgc tgccggtgct gcgcaaggat ttcatgctcg atccgtatca
     4141 ggtcgtcgaa gcgcgcgcga tgggcgcgga ctgcatcctc ctcatcgcgg cggcgatcga
     4201 cctgcccctg atgcgcgagc tggaggacgc cgcgctcgcg ctcggcatgg cggtgctggt
     4261 cgaagtgcac gacggcgcgg agctcgaccg ggcgctccgg ctgcgcacgc cgctcgtcgg
     4321 catcaacaat cgcgacctgc gcaccttcga aacgcggctc gagacgacgc tgcgattgca
     4381 tgcgcgcgtg agcgcgccgc gccttgtcgt ctccgaaagc ggcatcgcgg cacgagggga
     4441 cgtctcgaag ctgtggcagg c
//
LOCUS       DBNHKE010000017         6627 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_55741, whole genome shotgun sequence.
ACCESSION   DBNHKE010000017 DBNHKE010000000
VERSION     DBNHKE010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_55741"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="AC16O9_01095"
     CDS             <1..331
                     /locus_tag="AC16O9_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515246.1"
                     /translation="DPDPRAPNGFRIPFNAETGELIAARWKNWQRHDPINLVSRYKNN
                     LRSLRGIYIDCGWTDQYHIQYGSRILSRRLAEANLRHRYEEFPDDHSDIDYRMDVSLP
                     FLTRALR"
     gene            358..714
                     /locus_tag="AC16O9_01100"
     CDS             358..714
                     /locus_tag="AC16O9_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515247.1"
                     /translation="MSVRLVRAFVVAGAALGVSSCMSFVIEGAGDRDKTTAAQKVEET
                     IHGSFWGFLWVKPAVAKCEKNHELYRIKYHQNALYVLASAATLGAYVPQAIEWWCVPR
                     EDPKADEGPSLKPRPR"
     gene            711..1667
                     /locus_tag="AC16O9_01105"
     CDS             711..1667
                     /locus_tag="AC16O9_01105"
                     /inference="COORDINATES: protein motif:HMM:NF021508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HIR6515248.1"
                     /translation="MNVRNLIVGLDGTFNEPRQLDAGRVAPTNVAKLLSLLKRGQRIH
                     YEEGVGTGMWEQVRGGIIGYGLDERILGAYRFLCQRFADSDWRREDNRIFLFGFSRGA
                     YTARRISGLIAHSGIPVDVEDTELGFELYKGRDQVSVDKLKREGRFFDFPVEMVGVWD
                     TVKATNDADYHDDELCGNVVAGYHAMAIDEKRRVFPVLRWAADPRVTQLWFAGVHADV
                     GGGYHDAALSDIALEWMVWRGVDHGLDFSETKFRALNPRPGGRMHDSYSGIWKAFGSE
                     ARIVRAADPVHESVRERIEDAPAKYRPKLPSRPIFWKEPYRV"
     gene            complement(1694..2980)
                     /locus_tag="AC16O9_01110"
     CDS             complement(1694..2980)
                     /locus_tag="AC16O9_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6515249.1"
                     /translation="MTKPLHAFSAAEAARLIESRELTAEALAAACFERIAERDDAVRA
                     WAFIHEKQTLAAARELDRAPRRSRLHGVPFGIKDIIDTEDLPTEYGSPIYRGHRPRAD
                     AACVALLRRAGCLILGKTVTTEFANNHPSMTRNPHDPAHTPGGSSSGSAAAVADHMVP
                     LALGTQTGGSVIRPAAYCGAFALKPSFGSINRQGTKFVAESLDTIGIFSRSAEDLALA
                     LEVLSGRSVPDFASFTGKPRIGLCRTPRWQEADTLSQTNLEQAARLLAKAGATVSDFE
                     LPSGSEALFDRHSVIMGYESARALAWEYENHPGEISTSLKPRLEEGWQVARAEYDAVR
                     ETARQCRRALADRMREVDFLLTPSATSEAPRSHASTGDPVFNRAWTLLGVPCVTVPFG
                     KGAHGLPLGVQLVGPFDGDSALLGWAHWATAALGRG"
     gene            3107..3433
                     /locus_tag="AC16O9_01115"
     CDS             3107..3433
                     /locus_tag="AC16O9_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR6515250.1"
                     /translation="MLSIRVLEYLEPSGRSPFRHWFDDLEATTAARVGAYLYRLEEGN
                     FSRVEGVGAGVYELKVDFGPGYRVYFGKDGETVLILLGGSTKKRQRAAIGRAQAAWQD
                     YKRRKR"
     gene            3446..3766
                     /locus_tag="AC16O9_01120"
     CDS             3446..3766
                     /locus_tag="AC16O9_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing
                     transcriptional regulator"
                     /protein_id="HIR6515251.1"
                     /translation="MALTRDFKETVVERAQRDARFREAMFTQALNAYLAGDTRTGKAM
                     LRDLVNATIGFEGLAAEVRKPSKSLHRMLAERGNPSTENFFDIVQALQRRTRVRLHVT
                     AHAN"
     gene            3878..5497
                     /locus_tag="AC16O9_01125"
     CDS             3878..5497
                     /locus_tag="AC16O9_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HIR6515252.1"
                     /translation="MIVASGIAMQFGAKPLFTDVSVKFGGGNRYGLIGANGCGKSTFM
                     KILGGELEPSAGTIAIDSNERVGRLRQDQFAYETERVLDVVLMGHEKMWAAMQERDAI
                     YANESANEDDYLKAAELEQKYAEYGGYTAEARAGDLLLGLGVPTHQHHGPMSAVAPGW
                     KVRVLLAQALFGDPDILLLDEPTNNLDIHSIRWLEDVLNRRTSTMVIISHDRHFLSSV
                     CTHMADVDYGQIRIYAGNYDDYMEASTLAKQQLEKDNAKAKDRIADLEDFVRRFRAHK
                     AKARQATSRQKMIEKLKVEDVKPSSRQYPFIRFLVDPKEKLHRLALEAKALTKGYEKG
                     KPLFRNLDLAIEAGEKVAIIGPNGVGKTTLLRCLEGSLKPDQGTVKWAEKANRGYWPQ
                     DPADEFKGDESLADWMGRWRRKDKAQDELIVRATLGQLLFSGDDQKKSLKVISGGEAQ
                     RMLIGRLVLQMPNVMLLDEPTNHLDMESIESLNGALDKYTGTLIFVSHDREFVSSLAT
                     RILELKPGGEVIDFKGSYEDYLAGRLEALAA"
     gene            5514..6329
                     /locus_tag="AC16O9_01130"
     CDS             5514..6329
                     /locus_tag="AC16O9_01130"
                     /inference="COORDINATES: protein motif:HMM:NF021623.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6515253.1"
                     /translation="MHPIPVRTMKFDVPSAADFHPLYLAGSAALSYSHSAFGLYVALL
                     EPFAVKSLRKVLGKIRDDKLREEVDRFCRQEAQHYQRHIDFNKVILAHGYPGLEARIE
                     QLRQDLERVLGNASDRYCVGYVEGFESYTTQFALNMIGSGLYDHRRTHPAFGALFKWH
                     MLEEIEHRTVAFDLYQHLYGGALYRVRMCWISQGHMLRFLDDCADLMSKHDIARHGER
                     CRIGARQKFQLATFPIGMRIRSMLPGYTPRRHKVPEDIARLSAHFTQMAESVR"
     gene            complement(6301..>6627)
                     /locus_tag="AC16O9_01135"
     CDS             complement(6301..>6627)
                     /locus_tag="AC16O9_01135"
                     /inference="COORDINATES: protein motif:HMM:NF052063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6515254.1"
                     /translation="LGLWRHERARARREARAALHAACRRNDARGARDALLAWESAVGA
                     KAALEPAQAQALDAALYADRAWDGKAFWRALRLAKPKPRRAAPRAPLPPLFRLQAPSA
                     RSRPSA"
BASE COUNT         1182 a         2251 c         2211 g          983 t
ORIGIN      
        1 cgaccccgac ccgcgcgcgc ccaacggctt tcgcatcccg ttcaacgcgg aaacgggcga
       61 attgatcgcg gcgcgctgga agaactggca gcgccacgac cccatcaacc tggtttccag
      121 atacaagaac aacctaagat cgctgcgcgg catctacatc gactgcggct ggaccgacca
      181 gtaccatatc cagtacggct cacgcatcct ctcgcggcgc ctcgcggaag cgaacctccg
      241 ccaccgctac gaggaattcc ccgacgacca ctcggacatc gactaccgca tggacgtgag
      301 cctgccgttt ctcacgcggg cgctgcgctg agcacaggtt caccacagga gaccgacatg
      361 tccgtaagac tggttcgagc attcgtcgtc gccggcgccg cgcttggcgt ctcgagctgc
      421 atgagcttcg tgatcgaagg tgccggggat cgggacaaga ccacggccgc gcagaaggtg
      481 gaagaaacga tccacggatc gttctggggc ttcctctggg tgaagccggc ggtggccaag
      541 tgcgagaaga accacgagct ctaccggatc aagtaccacc agaacgcgct ctacgtcctc
      601 gcttcggccg cgacgctcgg ggcctacgtg ccgcaggcga tcgaatggtg gtgcgtgccg
      661 cgggaagacc ccaaagcaga cgaaggaccg tcgctgaaac cgcggccgcg atgaacgtgc
      721 gcaacctcat cgtcggcctg gacggaacgt tcaacgagcc gagacagctc gacgccggca
      781 gggtcgcgcc gaccaacgtc gcgaagctgc tgtcgctgct taagcgcggg cagcggattc
      841 actacgagga gggcgtgggg accgggatgt gggagcaggt ccgcggcggc atcatcggtt
      901 acgggctcga tgaaaggatt ctcggcgcgt accggtttct ctgccagcgg ttcgccgact
      961 cggactggcg gcgcgaggac aaccggatct tcctgttcgg cttcagccgc ggggcgtaca
     1021 ccgcgcggcg catcagcgga ttgatcgcgc acagcggcat tcccgtcgac gtggaggaca
     1081 ccgagctcgg cttcgagctc tacaagggcc gcgatcaggt cagcgtcgac aagttgaaga
     1141 gggaggggcg gttcttcgac ttccccgtcg agatggtcgg cgtctgggac acggtcaagg
     1201 cgaccaacga tgccgactac cacgacgacg agctctgcgg caacgtggtc gcgggctatc
     1261 acgccatggc gatcgacgag aagcgccggg tgttcccggt attgcggtgg gccgcggacc
     1321 cgagagtgac ccagctctgg tttgccggcg tccacgccga cgtcggcggc gggtaccacg
     1381 acgcggcgct ctccgacatc gccctcgaat ggatggtctg gcgcggcgtt gaccacggcc
     1441 tcgatttcag cgagacgaag ttccgcgctc tgaacccgcg tcccggcggc cgcatgcacg
     1501 actcctacag cggtatctgg aaggcgttcg gcagcgaggc gcgcatcgtc agagcggccg
     1561 acccggtcca cgaaagcgtg cgggaacgca ttgaagatgc gcccgcaaag taccggccga
     1621 agcttccctc gcggccgatc ttttggaagg agccgtatcg ggtgtgacgg ccgttcctcg
     1681 cccgggcgcc ggcctagccg cggcccagcg ccgcggtcgc ccagtgcgcc cagcctagca
     1741 gcgcgctgtc cccgtcgaac ggcccgacga gctgaacccc cagcggcagc ccgtgcgcgc
     1801 ctttgccgaa aggcacggtg acgcagggaa cgccgagcaa ggtccacgcg cggttgaaca
     1861 ccggatcgcc cgtgctcgcg tgcgagcgcg gcgcctcgct ggtcgcgctc ggcgtgagca
     1921 ggaaatcgac ttcgcgcatt ctgtccgcca gggcgcgccg gcattggcgc gcggtctcgc
     1981 gcacggcgtc gtactcggcg cgcgccacct gccacccctc ctcgaggcgc ggcttcaggc
     2041 tcgtgctgat ctccccggga tggttctcgt actcccaggc cagcgcccgc gcgctctcgt
     2101 agcccatgat gacactatgc cggtcgaaga gcgcctcgct cccggaaggc agctcgaagt
     2161 cgctcaccgt agcgccggcc ttcgcaagca gccgggcggc ctgctccaga ttagtctgcg
     2221 acaaggtgtc cgcttcctgc cagcgcggcg tgcgacacaa cccgatgcgc ggcttgccgg
     2281 tgaaggaggc gaagtccggg acggaacggc cggacaggac ttcgagcgcg agcgccaggt
     2341 cctccgcgct tcttgaaaag ataccgatcg tgtcgagcga ttcggcgacg aacttcgttc
     2401 cctggcggtt gatgctgccg aagctcggct tcagcgcgaa cgcgccgcag taggcggcag
     2461 gccggatcac cgagccgccg gtctgggtac ccaaggcgag cggcaccatg tggtcggcca
     2521 ccgcggccgc ggagccgctg gaagacccgc ccggcgtgtg cgcgggatcg tgcggattgc
     2581 gcgtcatcga gggatggttg ttggcgaact cggtggtgac cgtcttgccg aggatcaggc
     2641 acccggcgcg gcgcaggagc gcgacgcagg ccgcatccgc ccggggccgg tgcccgcgat
     2701 agatcggcga gccgtactcg gtcggcaggt cctcggtgtc gatgatgtcc ttgatgccga
     2761 agggcacgcc gtgcaggcgg ctcctgcgcg gcgcgcggtc gagctcgcgt gccgcggcga
     2821 gcgtctgctt ctcgtggatg aacgcccacg cgcgtaccgc gtcgtcacgc tccgcgatgc
     2881 gctcgaagca ggccgcggcg agcgcctcgg cggtcagctc gcgggattcg atcagacgcg
     2941 cggcctcggc cgcggaaaac gcatgcagtg gcttagtcat ttccggaggg tataacgccc
     3001 gaggcgttgc gcggctcagc tgggtaagtc atcccgccgg gcggggcccg cgttaggcgg
     3061 tcatgctgcc cgcaccgcgc ctggtaacac acgtgttacc atagccatgc tctcgatccg
     3121 ggtactggag tacctcgagc cgtccggtcg gtcgccgttc cgccactggt tcgacgacct
     3181 ggaggcgacg actgctgcca gggtcggcgc ctatctttac cggcttgagg aaggcaactt
     3241 ctccagagtc gagggcgtgg gtgcgggcgt ctacgaactg aaggtcgatt tcgggcccgg
     3301 gtaccgggtc tacttcggca aggacgggga gacggtgcta atcctgctgg gcggcagcac
     3361 caagaagcgc cagcgcgccg cgatcggccg ggcgcaggca gcctggcagg actacaagcg
     3421 gcgcaagaga tagcgaattc gagacatggc actgacgaga gacttcaagg aaacggtggt
     3481 cgagcgggcc cagcgggacg ccaggtttcg cgaggcgatg ttcacccagg cgctcaatgc
     3541 ctacctcgcc ggcgacacgc gcaccggcaa agcgatgctt cgtgacctgg tgaacgcgac
     3601 gatcggcttc gaaggtctcg ccgcggaggt caggaagccc tccaagagcc tgcaccgcat
     3661 gctcgcggag cgcgggaacc ccagcacgga gaacttcttc gacatcgtgc aggcgctgca
     3721 aaggagaact cgcgttcggc tgcacgtaac tgcgcacgcg aattgatctg acagcgacgg
     3781 ggaccgctgc acggcgcgcc tgctacaatg cgccctgcgc agtgagtccc aacccgaagc
     3841 tggccgcggc cggctttttt gttcccggaa tctcccagtg atcgtcgcct ccggcatcgc
     3901 catgcagttc ggggccaagc ccctgttcac cgacgtctcc gtcaagttcg gcggcggcaa
     3961 ccgctatggc ctgatcggcg ccaacggctg cggcaagtcc acgttcatga agatcctcgg
     4021 cggcgagctg gaaccgagcg ccggcaccat cgcgatcgac agcaacgagc gggtgggccg
     4081 gctgcgccag gaccagttcg cctacgaaac cgagcgcgtg ctggacgtgg tgctgatggg
     4141 ccacgagaag atgtgggccg cgatgcagga gcgcgacgcg atctacgcca acgagagcgc
     4201 gaacgaagac gactacctga aggccgcgga gctggagcag aagtacgcgg aatacggcgg
     4261 ctacacggcc gaagcccgcg ccggcgacct gctgcttggc ctcggcgtcc ccacccacca
     4321 gcaccacggc ccgatgagcg ccgtcgcccc gggctggaaa gtaagagtgc tgctcgcgca
     4381 ggcgctgttc ggcgacccgg acatcctgct cctggacgag ccgacgaaca acctcgacat
     4441 ccacagtatc cgctggctcg aagacgtgct caaccgcaga accagcacga tggtgatcat
     4501 ctcgcacgac cggcacttcc tctccagcgt ctgcacgcat atggcggacg tggactacgg
     4561 ccagatccgc atctacgccg gcaactacga cgactacatg gaagcttcca ccctggccaa
     4621 gcagcagctg gaaaaggaca acgccaaggc gaaggaccgc atcgccgacc tggaagactt
     4681 cgtgcgccgc ttccgcgcgc acaaggcgaa ggcgcggcag gcgacctcgc gccagaagat
     4741 gatcgagaag ctgaaggtcg aggacgtgaa gccctcgagc cgccagtacc cgttcatccg
     4801 cttcctcgtc gacccgaagg aaaagctcca ccgcctcgcg ctggaggcga aggcgctgac
     4861 caagggctac gagaagggca agcccttgtt caggaacctc gacctcgcca tcgaggcggg
     4921 cgagaaggtg gcgatcatcg gcccgaacgg cgtcggcaag accacgctcc tgcggtgcct
     4981 cgaaggcagc ctgaagcccg atcagggcac ggtgaaatgg gccgagaagg ccaatagagg
     5041 ctactggccg caggacccgg cggacgaatt caagggcgac gagtcgcttg cggactggat
     5101 gggccgctgg aggagaaagg acaaggccca ggacgagctc atcgtgcgcg cgacgctcgg
     5161 ccagctcctc ttctccgggg acgaccagaa gaagtccctg aaggtcatct ccggcggcga
     5221 ggcgcagcgc atgctcatcg ggcgcctcgt gctgcagatg ccgaacgtca tgctgctcga
     5281 cgagcccacc aaccacctcg acatggaatc gatcgagtcg ctgaacggcg cgctcgacaa
     5341 gtacacgggc acgctcatct tcgtctcgca cgaccgcgag ttcgtgtcct ccctcgccac
     5401 gcgcatcctc gagctgaagc ccggcggcga agtgatcgac ttcaagggca gctacgagga
     5461 ctacctcgcc ggaaggttgg aagcgctggc cgcctagcgc taagatggtg cgcatgcacc
     5521 ccatccccgt gcgcaccatg aagttcgacg tgccgagcgc cgccgacttc cacccgctgt
     5581 acctcgcggg cagcgcggcg ctctcctaca gccactccgc cttcggcctg tacgtggcgc
     5641 tgctcgagcc cttcgcggtc aagtcgctga ggaaagtcct cggcaagatc cgcgacgaca
     5701 agctccggga ggaggtcgac cgcttctgcc gccaggaggc gcagcactac cagcgccaca
     5761 tcgacttcaa caaggtaatc ctcgcgcacg gctacccggg gctggaagcg cgcatcgagc
     5821 aactgcgcca ggacctcgag cgcgtcctcg gcaacgcgag cgaccggtac tgcgtcggct
     5881 atgtcgaggg cttcgagtcc tacaccacgc agttcgccct caacatgatc ggcagcggcc
     5941 tgtacgacca ccgccgcacg cacccggcgt tcggcgcgct cttcaagtgg cacatgctgg
     6001 aggaaatcga gcaccgcacg gtcgccttcg acctgtacca gcacctctac ggcggcgccc
     6061 tctaccgggt gcgcatgtgc tggatctcgc aggggcacat gctgcgcttc ctcgacgact
     6121 gcgccgacct gatgtcgaag cacgacatcg cccgccacgg cgagcgctgc cgcatcgggg
     6181 cgcggcagaa attccagctc gccacgttcc ccatcggcat gcgcatccgc tccatgctcc
     6241 cgggctacac gccgcgccgc cacaaggtgc cggaggacat cgcccggctc tcggcgcact
     6301 tcacgcagat ggccgagagc gtgcgctagg cgcctgcagc ctgaagagcg gcggcagcgg
     6361 cgccctgggc gccgcgcgcc gcggcttcgg cttcgccagc cgcagcgcgc gccagaacgc
     6421 cttcccgtcc caagcgcgat ccgcgtagag cgcggcgtcg agcgcctgcg cctgcgcggg
     6481 ctcgagcgcg gctttcgcgc cgaccgcgct ttcccaggcg aggagcgcgt cgcgcgcgcc
     6541 gcgcgcatcg ttcctgcggc atgcggcgtg cagcgccgcg cgcgcttcgc gccgcgcgcg
     6601 cgcgcgctcg tgccgccaga ggccgag
//
LOCUS       DBNHKE010000018         7799 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1008437, whole genome shotgun sequence.
ACCESSION   DBNHKE010000018 DBNHKE010000000
VERSION     DBNHKE010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1008437"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="AC16O9_01140"
     CDS             complement(<1..724)
                     /locus_tag="AC16O9_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515255.1"
                     /translation="MSGKRLVAAFLLLIAAGCGGGGDAPPPPPDLSGVWAGSWQGSDP
                     SPGGLGPVSGTWGVEITQGESSASGPSELRGDVDCMDGQMQTNPDASSAVTGSLARAP
                     CATVTWTLTALNVTEGSASGSWFNTGTTGSGTLSGTRIARLGGPRIRFVNPPGATPGA
                     IVTVSGLRLSGGALTFNSALSPTIHSADATRIVATVPSGVASGPAKVTTGAGTAESPL
                     PFSVDVRSPPVVLGNATTAGTAP"
     gene            complement(931..1686)
                     /gene="gspN"
                     /locus_tag="AC16O9_01145"
     CDS             complement(931..1686)
                     /gene="gspN"
                     /locus_tag="AC16O9_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013376.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR6515256.1"
                     /translation="MSGGRVALLVVAGVAAYAIALVATAPASWLAHAIERASDRVVEL
                     REPTGSLWSGSGRLYARARAGSLLDLGGLRWETSWSEMHRLRLGVSLAIGGAQRPAEL
                     QLSPSGLSVRRLSVSAPAEVITGFVPALEGLGPRGTLRVRSEDMRIERDSTLGQAEIE
                     WTDVRLARSPELEFGSHIVRLRGSGPRVVIELSSLGGPIRLSGTGTWSREAGLDLSGA
                     AEHDAQQASAMGVFLQGMCTTYRANRCEFRVRR"
     gene            complement(1683..2204)
                     /gene="gspM"
                     /locus_tag="AC16O9_01150"
     CDS             complement(1683..2204)
                     /gene="gspM"
                     /locus_tag="AC16O9_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HIR6515257.1"
                     /translation="MNAFAALAALWRRFSARERTVIAAGALLAAAILLYVLLWEPGLA
                     ARRTLSSALPRLRAQLADMRLQREEIMALRRQIDAAARRGDLQTLLQASLRETAFAES
                     VERVSALPDGGAQVLIGSAAFDAWVEWLERAQREFGVRLQSATVTPPEPGGRVRIEAS
                     FAARGVPTAGSTR"
     gene            complement(2201..3433)
                     /gene="gspL"
                     /locus_tag="AC16O9_01155"
     CDS             complement(2201..3433)
                     /gene="gspL"
                     /locus_tag="AC16O9_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HIR6515258.1"
                     /translation="MSTLRIRVARDTPVSGEFRWARLDAPPGTPAAGSANLAEPGVTG
                     PCELVLAGDLVLLERVAVPRSQQKRLGESLRYLAEEMALQEPERLHVAAAPGPNRDSL
                     CLGVIERQWLQQLLARLQRAGLVAESAYPETLLPALAPRTWTVVWGGNESFVRTGEHD
                     ASMLDLAEAGAAPTALRLALEQARARGDAPQALVVRCAPEVATPDLAAWSQSLDLPVE
                     DGPAWHWSSAQPRPAFELLQGEFQSRRGAGAWVQRLRAAAVLAIALGAINSVALALDW
                     HAKARERDALLAQMRGLYRETFGEGAVVVDAPLQMQRALAQLRERSGRAGPSDFVTLA
                     SIAAGVLPPASQRQLERIAYDAGTLTLTLRSAAGQPPTALPAVLRARGAPPGYEVRIE
                     PPGSSGTLTVRLKAGGSP"
     gene            complement(3430..4353)
                     /gene="gspK"
                     /locus_tag="AC16O9_01160"
     CDS             complement(3430..4353)
                     /gene="gspK"
                     /locus_tag="AC16O9_01160"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HIR6515259.1"
                     /translation="MSPRTRQHGAAVLLAMLIVALAATAAASLMRQQDLALRQLSTAR
                     DYEQALWVLRGGAQWARSILAQDARTSAQDHRGELWATGLPTTEIEQGTVAGEILDQQ
                     GLFNVNNLVRDGKPSERDIAAFQRLLGIAGLEEGFAEALTDWIDPDAEARAGAGAEDD
                     YYLRLERPYRTPNQPVVEMGELLRVRGMDAAQLDRLRRYATALPRRTPVNVNLAPPEV
                     LAAVIPGLTLAEAEVMASNRAAAPFRNLEEFRAKLPRRELEWKEGELATDSRFFLVRG
                     RVTVGNADVRLEALLERDGSRMPLVLWQRMR"
     gene            complement(4350..4952)
                     /gene="gspJ"
                     /locus_tag="AC16O9_01165"
     CDS             complement(4350..4952)
                     /gene="gspJ"
                     /locus_tag="AC16O9_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HIR6515260.1"
                     /translation="MRARGFTLIELLAAIAVLAVLGAMAFRGLGSILEAESRVQEETR
                     RWGEVSLAFSQMNEDVLMAVGRTVQDAAGRLAPALRVGAAALERAEGDDAPLVLTRLG
                     SGEGAAAQSAPRRVGYRVRDGVLEYLAWPALDAAPAAAPQAYTLLDGVQGLTVEALDE
                     QGRWHPVWPAGRTVDALPRALKISLALAGGEEVTRMLALK"
     gene            complement(4954..5340)
                     /gene="gspI"
                     /locus_tag="AC16O9_01170"
     CDS             complement(4954..5340)
                     /gene="gspI"
                     /locus_tag="AC16O9_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HIR6515261.1"
                     /translation="MQAMAQAQPSRGFTLVEVLVALAIVAVALAAAARSVSVSTESAA
                     SHRSRVLAGFVAENQLAEFVARRAWPAIGTTQGLERQAGLEFAWRAEVIGTPHPLLRR
                     VEIRVMDPADPAHELRHLVGVLARER"
     gene            complement(5291..5836)
                     /locus_tag="AC16O9_01175"
     CDS             complement(5291..5836)
                     /locus_tag="AC16O9_01175"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR6515262.1"
                     /translation="MRASAAGWRPALGGFTLLEMLVVMSIVGVIGAMAAMSVSSADRG
                     RTRDEARRLATLLEFARTEARATGRSIAWLPLPEGYTFRRMAEDGTWVPFPEASPFRR
                     RTLPGTIYLHAVQVNAQPLRAEDFVVFSPYGASGELRVTVAGDGASLTLRAGMLGRIT
                     VSSDPHAGDGASAAQPRIHAG"
     gene            complement(5836..6306)
                     /gene="gspG"
                     /locus_tag="AC16O9_01180"
     CDS             complement(5836..6306)
                     /gene="gspG"
                     /locus_tag="AC16O9_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR6515263.1"
                     /translation="MRSARPLARARRPHSTRRGAGGFTLVEIMVVVAIMAILAALIVP
                     RIMSRPDEARVIAAQQDIRTIVQALKLYRLDNQRYPSTEQGLDALVKPPSQPPLPPNW
                     KAGGYLERLPLDPWRRPYQYLSPGIRGEIDVFSFGADGIGGGEGFDADIGSWSL"
     gene            6407..6901
                     /locus_tag="AC16O9_01185"
     CDS             6407..6901
                     /locus_tag="AC16O9_01185"
                     /inference="COORDINATES: protein motif:HMM:NF022800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR6515264.1"
                     /translation="MLRESWIAALELAIVVALALSLAVWTWQLFAPRTLAAPAPSSLG
                     AGPVGAAPLAARSLLGASGASASATVRAGSGGALTLVGVFAAPAPGTGRALIARGGDR
                     PQLVAAGEEIAPGVALNEVHADHVTVLRGGALERIELERRGAGGSQPLTPVQPQAPVR
                     PRED"
     gene            6901..>7799
                     /locus_tag="AC16O9_01190"
     CDS             6901..>7799
                     /locus_tag="AC16O9_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR6515265.1"
                     /translation="MRRALLAMLLGVAVGAGTARAADETVTLNFVNADIESVAAAIGK
                     ITNRNFLIDPRVRGTVNIVSARPVPASAVYGIFLSALRLQGFAAVESGGVVKILPEAD
                     AKLHAGASPARGATRGDRLQTQVYTLKYTSASQLLPVLRPIISPNNTIAAFAANNSLV
                     ITDYGENLRRIERIIEAVDLPGTGETAVVALQFASAADVVQTLNRVLADSMAPAGAQA
                     AADPTGRVFMAADPRTNSIVLRSENPARLEQAKALVGQLDVKTNAAANVHVVYLKNAE
                     AVVVAQTLRAILSGEAPAPVPATT"
BASE COUNT         1091 a         2885 c         2810 g         1013 t
ORIGIN      
        1 agggcgcggt gccggcggtc gtggcgttgc cgaggacgac ggggggcgac cgcacatcga
       61 cgctgaacgg caggggactt tccgcggtgc ccgcgccggt cgtcaccttc gccgggccgc
      121 tggcgacgcc gctcggcacc gtggcaacga tgcgcgtcgc gtccgccgag tggatagtgg
      181 gcgaaagcgc ggagttgaac gtcagcgcgc cgcccgacag ccgaagcccg ctcacggtga
      241 cgatggcgcc gggcgtcgcg ccgggcgggt tgacgaaccg gatgcgcgga ccgccaagcc
      301 gcgcgatgcg cgtgccgctg agcgttcccg atccggtggt gccggtgttg aaccagctgc
      361 cgctggcact gccttccgtg acattgagcg cggtgagcgt ccaggtcacg gtcgcgcacg
      421 gcgccctggc cagcgagccg gtcaccgcgc tgctcgcgtc cgggttcgtc tgcatctgcc
      481 catccatgca gtcgacgtca ccgcgcagct cgctcgggcc ggacgcggac gactcgccct
      541 gcgtgatctc gactccccag gtgccggaca ccgggccgag cccgcccggc gagggatcgc
      601 tgccctgcca ggaacccgcc cagactcccg aaaggtcagg cggcggcggc ggcgcgtcac
      661 cgccaccgcc gcaaccggcg gcaatcaaaa gcaggaacgc ggctaccagt cgcttgccgc
      721 tcacgaccct tgcgcgcatt acgccgctcc cgcctgtctg tttcggtcag gccatcctag
      781 gccgtcgctc gcactccgcc tatcccttga atgcggggga tggaatgcag tccggcatcc
      841 gcgcctgcac tttaggcgcg gatttcctgt gccatcttga ttttcgtcaa cgccttgcgg
      901 tttacatgcc ggcgcgaacg cccgcgaccc ctagcgccga acgcgaaact cacagcgatt
      961 ggcccggtac gtcgtgcaca tcccctggag gaacactccc atcgccgagg cctgctgcgc
     1021 gtcgtgctcg gcggcgccgg acaggtcgag accggcctcg cggctccagg tgccggtacc
     1081 cgacaggcgg atcgggccgc ccaggctgga gagctcgatc accacgcgcg ggccgctgcc
     1141 gcgcaggcgc acgatgtgcg agccgaactc gagctcgggc gagcgcgcga gccgcacgtc
     1201 ggtccactcg atttcggcct ggcccagggt ggagtcgcgc tcgatgcgca tgtcctcgga
     1261 gcgcacgcgc agcgtgcctc tcggccccag cccctccagc gcgggcacga agccggtgat
     1321 cacctcggcg ggcgcggaga ccgacaggcg gcgcaccgag aggcccgagg gcgaaagctg
     1381 gagctcggcg gggcgctgcg cgccgccgat cgcgagcgag acgccgagcc gcaggcggtg
     1441 catctcggac caggaggttt cccatcgcag tccgcccaga tcgagcaggc tgcccgcgcg
     1501 cgcccgcgcg tagagccgcc cgctcccgga ccacaggctt ccggtcggct cgcgcagctc
     1561 gacgacgcgg tccgacgcgc gctcgatcgc gtgcgcgagc cagctcgccg gcgcggtcgc
     1621 gacgagcgcg atcgcgtaag cggcgacgcc cgccacgaca agaagcgcca cgcggccgcc
     1681 gctcaccgcg tgctccccgc ggtgggcacg ccgcgcgccg cgaagctcgc ctcgatgcgc
     1741 acgcgcccgc ccggctcggg cggcgtgacc gtcgcggatt gcaggcgcac gccgaactcc
     1801 cgctgcgcgc gctcgagcca ctccacccag gcatcgaacg cggccgaccc gatgaggacc
     1861 tgggcgccgc cgtccgggag cgcgctgacg cgctccacgg actcggcgaa agcggtctcg
     1921 cgcaggctcg cctgcagcag cgtctgcagg tctccgcggc gcgcggccgc gtcgatctgc
     1981 cggcgcagcg ccatgatctc ctcgcgctgc agccgcatgt ccgcaagctg cgcgcgcagg
     2041 cgcggcagcg cggaggacag ggtccgcctc gcggccagcc ccggttccca gagcagcacg
     2101 tagaggagaa tcgcggccgc gagcagggca cccgcggcga tcaccgtgcg ttcccgcgcc
     2161 gagaagcggc gccagagcgc ggcgagcgcg gcgaaagcat tcatgggctg ccccccgcct
     2221 tcagccgcac ggtgagcgtg ccggaggacc cgggcggctc gatgcgcacc tcgtatcccg
     2281 gcggcgcgcc gcgcgcgcgc agcaccgccg gcagcgccgt cggcggttgg cccgccgcgg
     2341 acctcagggt cagcgtcagc gtgccggcat cgtaggcgat gcgctcgagc tggcgctggg
     2401 acgcaggcgg aagcacgccg gcggcgatgc tcgcgagcgt gacgaagtcc gatggtccgg
     2461 cccgccccga gcgctcgcgc aactgcgcca gcgcccgctg catctgcagc ggggcatcga
     2521 cgacgacggc gccctcgccg aaggtctccc ggtacagtcc gcgcatctgt gcgagcaacg
     2581 cgtcgcgctc gcgcgccttg gcatgccagt ccagcgcgag cgccactgaa ttgatcgcgc
     2641 cgagcgcgat ggcgaggacg gccgcggcac gcaggcgctg cacccaggcg ccggcgccgc
     2701 gccgcgactg gaactcgccc tgcagcagct cgaaagcggg gcgtggctgg gcgctcgacc
     2761 aatgccacgc cggaccgtct tcgacgggca ggtcgagcga ttgcgaccac gctgcgaggt
     2821 ccggggtggc gacctcgggg gcgcagcgca cgaccagtgc ctgcggcgca tcgccacggg
     2881 cgcgcgcctg ctcgagcgcg aggcgcagcg ccgtgggcgc cgcgcccgcc tcggcgagat
     2941 ccagcatcga agcgtcgtgc tcgccggtgc gcacgaagct ctcgttgccg ccccacacca
     3001 ccgtccaggt gcgcggcgcg agcgcgggca ggagggtttc cgggtacgcc gattcagcga
     3061 ccaggccggc gcgctgcaac cgggcgagca gctgctgcag ccactggcgc tcgatgacgc
     3121 ccaggcagag cgaatcgcgg ttcgggcccg gcgcggcggc gacgtgcagg cgctcgggct
     3181 cctgcagcgc catctcctcg gccaggtagc gcagcgactc accgagccgc ttttgctgcg
     3241 agcggggcac cgccacgcgc tcgagcagga cgagatcgcc ggcaagcacc agctcgcacg
     3301 ggccggtaac gcctggctcg gcgaggttcg cgctgcccgc ggcaggcgtg cccggcggcg
     3361 cgtccagccg cgcccagcgg aattcgcccg agaccggggt gtcgcgcgcg acgcggatgc
     3421 gcagcgtgct catcgcatcc gctgccacag cacgagcggc atgcggctgc cgtcgcgctc
     3481 cagaagcgcc tccaagcgca cgtcggcgtt gccgacggtg acgcgaccgc gcacgaggaa
     3541 gaaccggctg tcggtggcga gctcgccttc cttccattcg agctcgcgcc gcggcagctt
     3601 ggcccggaat tcctccaggt tgcgaaacgg cgcggcggcc cggttgctcg ccatgacttc
     3661 ggcctcggcc agcgtgagcc ccggaatcac cgcggcgagc acctcgggcg gcgccagatt
     3721 gacgttgacc ggcgtgcgcc gcgggagcgc cgtcgcatag cggcgcaggc gatcgagctg
     3781 cgcggcatcc atgccgcgca cccgcagcag ctcgcccatt tcgaccaccg gctgattcgg
     3841 cgtgcggtag ggccgctcca gacgcaggta atagtcgtcc tcggcgccgg cgcccgcgcg
     3901 ggcctccgcg tcggggtcta tccagtcggt gagcgcctcc gcgaatccct cctcgagacc
     3961 cgcgatgccg agcagtcttt ggaacgccgc gatatcacgc tcgctcggct tgccgtcgcg
     4021 caccagattg ttcacgttga acaggccctg ctgatcgagg atttcgccgg ccacggtgcc
     4081 ctgctcgatc tcggtggtcg gcaggcccgt ggcccaaagc tcgccgcggt gatcctgcgc
     4141 gctggtgcgc gcgtcctgag cgaggatcga gcgcgcccat tgcgcgccgc cgcgcaggac
     4201 ccagagggcc tgctcgtagt cgcgggccgt ggaaagctgg cgcagcgcga ggtcctgctg
     4261 gcgcatgagg gaagccgcag ccgtagccgc cagcgccacg atcagcatcg cgaggagaac
     4321 ggcggccccg tgctggcgcg tgcgcgggct catttgagcg cgagcatccg cgtgacctcc
     4381 tcgccgccgg ccagcgcgag gctgatcttg agcgcgcgcg gcagcgcgtc caccgtgcgg
     4441 cccgcgggcc agaccggatg ccagcggccc tgctcgtcga gcgcttcgac cgtgaggccc
     4501 tgcacgccgt cgagcagcgt gtaagcctgc ggcgcggcgg ccggcgcggc gtcgagcgcg
     4561 ggccaggcca ggtactcgag gacgccgtcg cgcacccggt agccgacgcg gcgcggcgcg
     4621 ctttgcgccg ccgcgccctc gccgcttccc agccgcgtga ggacgagcgg cgcatcatcg
     4681 ccctcggcgc gctcgagcgc cgccgcgccg acccgcagcg ccggcgcgag gcgcccggcg
     4741 gcgtcctgca ccgtccggcc gaccgccatc agcacgtctt cgttcatctg cgaaaacgcg
     4801 agcgacacct caccccagcg gcgcgtctct tcctgcacgc gggattcggc ctccaggatc
     4861 gagcccaggc cgcgaaacgc catcgccccg aggaccgcga gcaccgcgat cgcggcgagc
     4921 agctcgatga gcgtgaagcc gcgcgcgcgc acctcaacgc tcccgggcga gcacgccgac
     4981 caggtggcgc aattcgtgag cgggatcggc gggatccatc acgcggattt ccacccgcct
     5041 gagcagcgga tgcggcgtgc cgatcacctc ggcgcgccac gcgaattcga gcccggcctg
     5101 gcgctcgagg ccctgcgtcg tgccgatcgc gggccaggcg cggcgggcga cgaactcggc
     5161 cagctggttc tcggcgacga atccggcgag cacgcgcgag cggtgcgacg ccgcggactc
     5221 ggtcgacacc gataccgagc gcgcggccgc ggccagcgcg accgcgacga tcgcgagcgc
     5281 gaccaggact tcaaccagcg tgaatccgcg gctgggctgc gcttgcgcca tcgcctgcat
     5341 ggggatcgct actgacggta atccggccga gcatgccggc gcgcaaggtg agactcgccc
     5401 cgtcgccggc caccgtgacg cgcagctcgc cgctcgcgcc gtagggcgag aacaccacga
     5461 aatcctctgc ccgcagcggc tgcgcgttga cctgcaccgc gtgcaggtaa atcgtcccgg
     5521 gaagcgtgcg ccgccggaag gggctggctt ccggaaacgg aacccaggtg ccgtcctcgg
     5581 ccatgcggcg gaacgtgtag ccctcgggca ggggcagcca ggcgatgctg cggccggtcg
     5641 cgcgcgcctc ggtccgggcg aactcgagca gcgtcgcgag ccggcgcgct tcgtctcgcg
     5701 tgcgcccccg gtcggcagag gacacgctca tcgccgccat cgcgccaatc acgccgacga
     5761 tgctcatcac caccagcatt tcgagcagcg tgaagccgcc gagtgcgggg cgccagccag
     5821 ctgcggatgc gcgcactaga ggctccagga gccgatgtcg gcgtcgaagc cttccccgcc
     5881 gccgatgccg tcggcgccga agctgaacac gtcgatctcg ccccggatgc cgggactgag
     5941 gtactggtaa gggcgcctcc acggatcgag cggcaggcgc tcgaggtatc cgccggcctt
     6001 ccagttgggc ggcagcggcg gctggcttgg cggcttgacc agcgcgtcca gtccctgctc
     6061 ggtgctcggg tagcgctggt tgtccaggcg atagagcttg agcgcctgca cgatggtgcg
     6121 gatgtcctgc tgggcggcga tgacgcgcgc ctcgtcgggc cggctcatga tgcgcggcac
     6181 gataagcgcg gcgaggatcg ccatgatcgc gaccacgacc atgatctcga cgagcgtgaa
     6241 cccgcccgcg ccccgccgcg tgctgtgcgg ccgtcgggcc cgggcgaggg ggcgtgcgct
     6301 gcgcatggcc ggtgggtacc cggtgatttc gatcggtgta ctataccttg agccccggat
     6361 cgcgttgctg tggggtcaca tcggtatgca aagactagtc cactggatgc ttcgcgagtc
     6421 gtggattgcg gccctggagc tcgcaatcgt cgtggcgctt gcgctgagcc tcgccgtgtg
     6481 gacgtggcag ctgttcgcgc cgcgcacgct ggcggcgccc gcgccttcgt ccctcggcgc
     6541 ggggccggtc ggcgccgcgc cccttgccgc gcgcagcctg ctcggcgcga gcggggcaag
     6601 cgcgtccgcg accgtgcggg ccggaagcgg gggcgcgctc acgctggtgg gcgtgtttgc
     6661 ggcgcccgcg ccgggaacgg ggcgcgccct gatcgcgcgc ggcggggaca ggccgcagct
     6721 cgtggcggcc ggcgaggaaa tcgccccggg agtggcgctg aacgaagtgc acgccgacca
     6781 cgtcaccgtc ctgcgcggcg gcgcgctcga gcgcatcgag ctggagcgtc gcggcgcggg
     6841 cggctcacag ccgctgacgc ccgtgcagcc gcaggcgccc gtgcgcccgc gcgaagactg
     6901 atgcgccgcg cgctgctcgc catgttgctc ggggtcgcgg ttggcgcggg gacggcgcgc
     6961 gccgccgacg agacggtgac gctcaatttc gtcaatgccg acatcgaatc cgtcgccgcg
     7021 gcgatcggca agatcaccaa ccgcaatttc ctgatcgatc cgcgcgtccg gggcacggtc
     7081 aacatcgtct cggcgcggcc ggttccggcc tcggccgtct acggcatatt cctctcggcg
     7141 ctgcggctgc agggcttcgc cgcggtggag agcggcggcg tggtcaagat cctgcccgag
     7201 gccgacgcca agctgcacgc cggcgcctcg ccggcgcgcg gggccacgcg cggcgatcgc
     7261 ctgcagaccc aggtctacac gctcaagtac acctcggcga gccagctgct cccggtcctc
     7321 aggccgatca tcagcccgaa caacaccatc gcggcgttcg ccgccaacaa ctcgctcgtc
     7381 atcacggact acggcgagaa cctgcgccgc atcgagcgca tcatcgaggc ggtcgacctg
     7441 cccggaaccg gcgagaccgc ggtcgtcgcg ctgcaattcg cctcggcggc cgacgtcgtg
     7501 caaaccctga accgcgtgct cgcggacagc atggccccgg cgggcgcgca ggcggccgcg
     7561 gatcccacgg gccgcgtgtt catggcggcc gatccccgca ccaacagcat cgtgctgcgc
     7621 tcggagaacc cggcccgcct cgagcaggcc aaggcgctcg tcggccagct cgacgtgaag
     7681 accaatgccg ccgccaacgt tcacgtcgtc tacctcaaga atgcggaggc cgtggtggtg
     7741 gcgcagacgc tgcgggccat cctgtcgggc gaggcgccgg cgcccgtgcc ggcaacgac
//
LOCUS       DBNHKE010000019         2174 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1166721, whole genome shotgun sequence.
ACCESSION   DBNHKE010000019 DBNHKE010000000
VERSION     DBNHKE010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1166721"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC16O9_01195"
     CDS             complement(<1..290)
                     /locus_tag="AC16O9_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6515266.1"
                     /translation="MTIRIAPSILSADFARLGEEVRAVQAAGADLLHFDVMDNHYVPN
                     LTVGPLVCAALKPHVRLPIDVHLMVKPVDRIIPEFARAGASIISFHPEATEHV"
     gene            316..699
                     /gene="apaG"
                     /locus_tag="AC16O9_01200"
     CDS             316..699
                     /gene="apaG"
                     /locus_tag="AC16O9_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR6515267.1"
                     /translation="MGQEKRYEVAVGAATRYLADQSDESAGRYVFAYTITIRNAGSVA
                     AQLISRHWIITDAQGLVQEVRGLGVVGAQPMLKPGESFEYTSGTAIATPVGTMRGAYQ
                     MVAEDGTRFEAPIPEFTLSVPRVLH"
     gene            800..1930
                     /gene="mltA"
                     /locus_tag="AC16O9_01205"
     CDS             800..1930
                     /gene="mltA"
                     /locus_tag="AC16O9_01205"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR6515268.1"
                     /translation="MGLILLGACAQVPPPCPCPPPKPATDARYEPVAFAALPGWSAAP
                     LAASVRAFVAGCARVAPGQVMHEACEAARRLPAGDEAAARAFYQDAFEAYALVAPDGA
                     REGMVTGYYEPVLEGSRGPSARFAQPVHGVPDDLVVVDLASQYPELRGMRLRGRLDGR
                     RLRPYYSRGEILTRNGALPAPVIAWVADPVELFFLQVQGSGQIQLASGERIRVGFAEQ
                     NGHPYRSLGRHLVERGELPLEQASMQGIKAWAAANPARLQEALSHNASYVFFRELPAV
                     DGPIGAHGVPLEAGYSIAVDPKFVPLGAPVFLATTYPLSAQPLERLVMAQDTGGAIRG
                     AVRADFFWGSGAEAGALAGRMRQPGRLWVLWPRGAPLPRPLP"
     gene            complement(1934..>2174)
                     /locus_tag="AC16O9_01210"
     CDS             complement(1934..>2174)
                     /locus_tag="AC16O9_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HIR6515269.1"
                     /translation="VWCSADRAKAWLALAARAKAQVPTGKADCETPIERVLELGRSLG
                     VNSTPTLFFATGERARGGMQLPDLRAKLDEAARAR"
BASE COUNT          286 a          785 c          789 g          314 t
ORIGIN      
        1 acgtgctcgg tggcctcggg atggaagctg atgatgctcg cgccggccct ggcgaactcg
       61 ggaatgatgc gatccacggg cttcaccatc aggtgcacgt cgatcggcag gcgcacatgg
      121 ggcttcagcg ccgcgcacac gagcgggccg acggtgaggt tgggcacata gtggttgtcc
      181 atgacgtcga aatgcagcag gtcggcgccg gcggcctgca ccgcgcgcac ctcctcgccc
      241 agcctggcga aatccgccga caggatgctg ggcgcgatgc ggatggtcat ggcggcgcga
      301 gtttacaatg cgcccatggg tcaggagaag cgctatgaag ttgccgtcgg cgcggcgacg
      361 cggtacctcg ccgaccaatc cgacgaaagc gccggccgct acgtgttcgc ctacaccatc
      421 accatccgta acgccggcag cgtcgccgcg cagctgatca gccggcactg gatcattacc
      481 gacgcgcagg gcctggtgca ggaggtgcgc ggcctcggcg tggtcggcgc gcagccgatg
      541 ttgaagcccg gcgagagctt cgagtacacg agcggcaccg cgatcgcgac gccggtcggc
      601 accatgcgcg gcgcctacca gatggtggcc gaggacggca cgcgcttcga ggcgccgatc
      661 ccggagttca cgctgtcggt gccgcgggtg ctgcactgag acttactcgt cgtctccggt
      721 ctttcgcggc caggtccacc acacgatgag gatgagcagc gccagcgcca ccagcatttc
      781 gaggatcacg acccacatcg tggggctgat tctactgggc gcgtgcgcgc aggtgccgcc
      841 gccctgcccc tgcccgccgc ccaagcccgc cacggacgcg cgctatgagc cggtcgcgtt
      901 tgccgcgctg ccgggctgga gcgccgcgcc gctggccgcg agcgtgcgcg cgttcgttgc
      961 gggctgcgcg cgcgtcgccc ccgggcaggt catgcacgaa gcgtgcgagg ccgcgcggcg
     1021 gctgcccgcc ggcgacgagg ccgccgcgcg cgccttctac caagacgcct tcgaggccta
     1081 tgcgctggtc gcgcccgacg gcgcgcgcga gggtatggtc accggctact acgagcccgt
     1141 gctcgaaggc agccgcggcc cgagcgcgcg ctttgcgcag ccggtgcacg gcgtgccgga
     1201 cgatctggtc gtggtggatc tcgcgtcgca gtatccggag ctgcgcggca tgcgcctgcg
     1261 cgggcggctc gacggccggc ggctgcggcc ctactactcc cgcggcgaga tcctgacgcg
     1321 caacggcgcc ctgccggcgc ccgtgatcgc ctgggtcgcg gatccggtcg agcttttctt
     1381 cctgcaggtg cagggctccg gccagatcca gctcgcctcg ggcgagcgca tccgcgtcgg
     1441 cttcgccgag cagaacgggc acccctaccg ctcgctcggc cggcacctcg tcgagcgcgg
     1501 cgagctgcct ctcgagcagg cttccatgca gggcatcaag gcctgggccg ccgccaaccc
     1561 cgcgcgcctg caggaggcgc tcagccacaa cgcgagctac gtgttctttc gggagctgcc
     1621 cgcggtggac gggccgatcg gcgcccacgg cgtgccgctg gaggccggct acagcatcgc
     1681 ggtggacccc aagttcgtgc cgctgggtgc gcccgtgttt ctcgccacga cctatccgct
     1741 ctcggcgcag ccgctcgagc ggctggtgat ggcgcaggac acgggcggcg cgatccgcgg
     1801 cgcggtgcgc gcggacttct tctggggctc gggcgccgaa gcgggcgcgc tcgccgggcg
     1861 catgcgccag cccgggcgcc tgtgggtgct gtggccgcgc ggcgcgccgc tgccgcggcc
     1921 gctgccctag cgcctacctg gcgcgcgcgg cctcgtcgag cttcgcgcgc aggtccggca
     1981 gctgcatgcc gccgcgcgcg cgctcgcccg tggcgaaaaa gagcgtcggc gtcgaattca
     2041 cgccgagcga acggccgagc tccagcacgc gctcgatcgg cgtctcgcaa tcggcttttc
     2101 cggtcggcac ctgcgccttg gcgcgcgcgg cgagcgcgag ccaggccttg gcgcgatccg
     2161 ccgagcacca gacc
//
LOCUS       DBNHKE010000020         9839 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_214583, whole genome shotgun sequence.
ACCESSION   DBNHKE010000020 DBNHKE010000000
VERSION     DBNHKE010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_214583"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..558
                     /locus_tag="AC16O9_01215"
                     /pseudo
     CDS             109..558
                     /locus_tag="AC16O9_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            745..858
                     /locus_tag="AC16O9_01220"
     CDS             745..858
                     /locus_tag="AC16O9_01220"
                     /inference="COORDINATES: protein motif:HMM:NF023816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein C-terminal
                     domain-containing protein"
                     /protein_id="HIR6515270.1"
                     /translation="MIADGAPAEVIKDPKVIEAYLGSSDYARPPGTAHAAA"
     gene            845..1597
                     /locus_tag="AC16O9_01225"
     CDS             845..1597
                     /locus_tag="AC16O9_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515271.1"
                     /translation="MPLLEVKDLGVRYDKAVILNGVSLSVGEGEFVGIVGPNGAGKST
                     VLRAISGLIRLQAKMQRGTLGEVVLDGEIRFAGERIDTLPAHEVRRRGFALCPGRQRP
                     FRELTVLENLKAGAHLSRDAAQTRRRLERCFALFPRLAERKSQISGTLSGGEQQMLAT
                     ARALMYEAKLFAIDEPSLGLAPKVREEVFATISRIHAEGIPVLVVEQEVGQVFKLAHR
                     NYVLSQGRVWGEGAGAALLADESLRACYLGML"
     gene            complement(1598..2674)
                     /locus_tag="AC16O9_01230"
     CDS             complement(1598..2674)
                     /locus_tag="AC16O9_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6515272.1"
                     /translation="MYWLRRLWMQLRTEVLVLPSRTLVLAWAVALLALPLAYADPYVL
                     RILTLMCLFTIFAASWDLLAGYSGQVNFGHALFFGAGAYVSAMLALKLGWSPWLTVPA
                     GTAVATVLGAGVGYLCLRLRGSYLSLATLALPLIALGLLFAFPGFSGGELGFSGLPRL
                     TNNLLGNYYIAVIAMLALVFGMWLLADSRFGLVLHAIRDDELAARASGINTPRYKVAV
                     FAVSASAAGLAGALLAHYMRVTGPFTLETTLSFQAVIWGIFGGVATIYGSVVAVFVLY
                     PITEWIGGFQAFGERRLLVFAVIVLQVLLFMPRGLAPWVRDRIETLCPRCKQRNGAWR
                     SHCRLCSAQLIPTDRPRASEPGKG"
     gene            complement(2674..3546)
                     /locus_tag="AC16O9_01235"
     CDS             complement(2674..3546)
                     /locus_tag="AC16O9_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6515273.1"
                     /translation="MFQDILVTGLVNGSVYALLAIGFSLIFGVARIVNVAHTAFYMLG
                     AYLFYTLLVKLGLNFALAGVIAVAAITALSLVCYKLIIEPLREHEAAVLIATIALAII
                     IQELMLLAFGGHYLGIPSTLEGTVRIAGVSIAYQRFVILVVAFVMLAGTWYLLYATRL
                     GLAIRATANDLEVANLMGMNVDRVALATVGFSVALAAIAGVVVAPVFVVDPLMWLGPL
                     VTMLAIVVLGGLGSVKGSLVGAFIIGFVEAITVFAVPAGAFLKGAVALSIMVLVLLLR
                     PEGLYGVVFEEEER"
     gene            complement(3604..4821)
                     /locus_tag="AC16O9_01240"
     CDS             complement(3604..4821)
                     /locus_tag="AC16O9_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6515274.1"
                     /translation="MLNRFVLAAALALASAAHAQAPIKIAVLGPMAFVQGQHHWMGAE
                     MARDEINKAGGISVGGKKVMLELIRADSNEMTSVPDAASAVERVITRDKVDFLIGGFR
                     SEAVLAMQEVAMDHKKLFVGVGAAHDELGVRVEKDYNRYKYWFRVSPTKSTDLGRTVF
                     GVLGAVAGEIRTRLKTETPKVAVLAEKAVWTEGLIAAAKKNLPAMKMEMVGLWQPSAV
                     ATDVNAELSAIERAGADIVFTVLSGPVGIVVGRQMGERNMKAVAFGINVEAQKDEYWA
                     AAAGKANYLSTLNTYAEVEITPKTIPWIRAFRERFKMTPTYTAATYDAIMLIKEAVER
                     ANSTSADDMVPVLEKISYVGNGAVLEFDKRHDPVWGVGKSLGIGVQWQDGRLVAFWPP
                     PIKGMQPFRLPAR"
     gene            complement(4899..5057)
                     /locus_tag="AC16O9_01245"
     CDS             complement(4899..5057)
                     /locus_tag="AC16O9_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515275.1"
                     /translation="MPRVDFPRLLQLYMGGKLKLDELITRRHSIDEAPQAFADLESRK
                     NARGVIVF"
     gene            complement(5079..5345)
                     /locus_tag="AC16O9_01250"
     CDS             complement(5079..5345)
                     /locus_tag="AC16O9_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515276.1"
                     /translation="MKPWFEMPRVSMPFAFPLAIIGSSTPRAARHLERDVQVEVVLVL
                     ELEGHVGRLEEGEVRAVVEPVERVRHAGLAAGFVQRNAQGSSYL"
     gene            complement(5453..6475)
                     /locus_tag="AC16O9_01255"
     CDS             complement(5453..6475)
                     /locus_tag="AC16O9_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6515277.1"
                     /translation="MEQDTLSEVLRSIRLRGTVFFHLQGRAPWVVESPPTSEIASAVI
                     PGAEHVMAYHVVTRGSCWVGVVGEQPALVREGDIVVFPHGDRHVLSSTPHMRARQAVD
                     PNLFPARIGPPFFVDVHGDQPTLVAPEADGGSGRAVLVCGFLGCDVRPFNPLIASLPR
                     LLIERAGQGSPEGWLAQFVRSCGAGEGKLRPGDGAVLERMSEMMVLDLVRRHLERLPE
                     TQGGWLGGLRDRFVGRALALMHAHPDRAWTIDRLADETGLSRSALHERFVQFTGQAPM
                     QYLAGWRMQLASGLLRQSDADVTSIALEVGYESVAAFSRAFKRATGLAPTAWRKDAAA
                     NRPGPG"
     gene            6705..7559
                     /locus_tag="AC16O9_01260"
     CDS             6705..7559
                     /locus_tag="AC16O9_01260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6515278.1"
                     /translation="MTNTTTNAAASPAGAAAQPDFAAIKERQKAVWNAGDYAVLGGTL
                     QIVGELLCEAVDLRAGENVLDVAAGNGNASLAAARRWAAVTSTDYVAQLLEKGRARAA
                     ANGMPIEFKVADAENLPFDDASFDVTLSTFGVMFAPNQDRAASELLRVTRPGGRIGLA
                     NWTPTSLIGATLKTVGRYVPPPPGLKPPLLWGTEPRIVELFGPAAADIKVQRRDFVFR
                     YRSARHWLDVFTAYYGPLLKAIESLDESGRAALLGELEALLGQSNRSGSDSLVAPSEY
                     LEIVITRA"
     gene            7962..9335
                     /locus_tag="AC16O9_01265"
     CDS             7962..9335
                     /locus_tag="AC16O9_01265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6515279.1"
                     /translation="MPNEAMQKLKGNIKGRVILPEDRNYDDVRKIWNAMIDRRPAVIV
                     QCASPGDARHAIQYARDQGLDIAIRGAGHNIAGNGVCEGGLLIDHSTMKGVRVDAAKR
                     RAYVEPGATLGDFDAAVQAHGLATPVGINSTTGIAGLTLGGGFGWLTRKHGMTIDNLV
                     AANVTLANGKEVRASADEHADLFWAIRGGGGNFGVVTQFEFALHPVGPEVFAGLMVFP
                     GAQAKSVLKKYRDFVASAPEELCVWVVLRKAPPLPFLPENVHGKDVVVLALCYAGDVA
                     QGEKLIEPMRGFGDAYGTHVGAMPYAGWQQAFDPLLTPGARNYWKSHNLQSLGDAAVD
                     SILAYAGKLPSAQCEIFIAHIAGAANRVRADAMAYGHRDAKFVLNVHGRWDAAADDKA
                     CIGWAREFFAASAPYASAGAYINFMTEDETDRVQAAFGANHARLATIKKQYDPENIFH
                     LNQNIKP"
     gene            9547..9720
                     /locus_tag="AC16O9_01270"
     CDS             9547..9720
                     /locus_tag="AC16O9_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515280.1"
                     /translation="MPGQSSALAPLAVVTGGARGIGLAIAERFLAEGHRVALLDIDRG
                     TLRATEKRLNNQR"
BASE COUNT         1729 a         3372 c         3277 g         1461 t
ORIGIN      
        1 gcgggaggag cgcgcctagc cgcgcccccg cctttggaac gcccccgagc ccgttccgtg
       61 tctgcgccct tctttcgcgt cgaacgcctg accaagcgtt tcggcggcct ggtcgccctc
      121 aacgacatca gcttcgagct gcgccgcgac caggtcaccg gcctgatcgg cccgaacggc
      181 tcgggcaaga ccaccctgct gcggctcatc accggcatcc tcaagcctga cagcgggcgc
      241 atcgaattca acggcgaaga catcacgaac ctgaaaccgt gggacgtgac caatcgcggc
      301 atcgccggca ccttccagat gatgcgcccg ttccgccatc tgccggtcat cgccaacgtg
      361 atggtgccgc tgcttgcgcc gcgggcgaag cgacgcggtg aatgggtgaa gaagatcgaa
      421 gccaaggcga tggacgcgct ggaattcgtc ggcatctccg aactggccct ggagtccgcc
      481 tcggggctgt cgcagggcga actcaagcgc ctggaggtgg cgcgcgcgat ctcggccgag
      541 ccggagttgc tgctgctcga tgagccgctc ggcggcatcg gcccggtcga ttccgggttg
      601 ctggtgaaat cgatcctccg gatccacaag ggcggccgct tcggcagact gcactcggaa
      661 gggcccgccg tgctgatggt cgagcacaag ctgaaggaac tcatgtccat cgtggatcgc
      721 gtcatcgtgc tcgaccacgg cgagatgatc gccgacggcg caccggccga ggtcatcaag
      781 gacccgaagg tgatcgaggc ttatctcggc agcagcgact acgcaagacc cccgggaacc
      841 gcgcatgccg ctgcttgaag tgaaagacct cggcgttcgc tacgacaagg cggtgatcct
      901 caacggcgtc tcgctctcgg tcggcgaggg cgagttcgtc ggcatcgtgg gcccgaatgg
      961 cgccggcaag tcgaccgtgc tgcgcgccat ctccggcctg atccggttgc aggcgaagat
     1021 gcagcgcggc acgctcggcg aggtcgtgct ggacggcgag attcgcttcg ccggtgagcg
     1081 catcgacacg ctgcctgcgc acgaagtccg ccggcgcggg ttcgcgctct gtcccggccg
     1141 gcagcggccg tttcgcgaac tcaccgtgct cgagaacctg aaggcgggcg cgcatctttc
     1201 gcgcgacgcg gcgcagacgc gccgccgcct cgagcgctgc ttcgcactgt ttccgcgcct
     1261 ggcggagcgc aagagtcaga tatcgggcac gctgtcgggc ggcgagcagc agatgctcgc
     1321 cactgcgcgg gccctcatgt acgaggcgaa gctgttcgcg atcgacgagc cctcgctcgg
     1381 gctggcgccc aaggtgcgcg aggaagtctt cgccaccatc tcgcgcatcc atgccgaggg
     1441 catcccggtg ctcgtcgtcg agcaggaagt cggccaggtg ttcaagctgg cgcaccgcaa
     1501 ctacgtgctg tcgcagggcc gggtgtgggg cgaaggtgcc ggcgccgcgc tgctggccga
     1561 cgagtcgctg cgcgcctgct acctggggat gttgtagcta ccccttgccg ggttcggagg
     1621 cgcgcggccg gtcggtgggg atgagctgcg cggagcacag gcggcagtga gagcgccagg
     1681 cgccgttgcg ctgcttgcag cgcgggcaca gggtttcgat ccggtcgcgc acccagggcg
     1741 caagaccccg cggcatgaac agcagcacct gcagaacgat caccgcgaag accagcagcc
     1801 gccgctcgcc gaacgcctgg aagccgccga tccactcggt gatcggatag agcacgaaca
     1861 ccgccaccac cgatccatag atcgtcgcca cgccgccgaa gatcccccag atcaccgcct
     1921 ggaacgacag cgtcgtctcg agcgtgaacg gtccggtgac cctcatgtaa tgagccagca
     1981 gcgcccccgc gagtcccgcg gcagacgccg acaccgcgaa caccgcgacc ttgtagcgtg
     2041 gcgtgttgat tccagaggcg cgcgccgcca gctcgtcgtc gcggatcgcg tgcagcacca
     2101 ggccgaacct cgagtcggcg agcagccaca tgccgaagac cagcgcgagc atcgcgatca
     2161 ccgcgatgta atagttgccc agcaggttgt tggtgagccg gggcagtccg gagaatccca
     2221 gctcgccacc ggaaaaaccg ggaaacgcga acagtagccc gagcgcgatc agcggcaggg
     2281 cgagcgtcgc gagcgagaga taggagccgc gcaggcgcag gcacagatac cccaccccgg
     2341 cgccgagcac cgtcgccacc gcggttcccg ccgggaccgt gagccacggc gaccacccga
     2401 gcttgagcgc aagcatcgcc gagacgtagg cgccggcgcc gaaaaaaagc gcgtggccga
     2461 aattcacctg gcccgagtag ccggcgagca gatcccagct cgcggcgaag atcgtgaaca
     2521 ggcacatcag ggtgaggatc cgcagcacat agggatcggc gtaggcgagc ggaagcgcga
     2581 gcagcgcgac ggcccaggcg agcaccagcg tgcgcgaggg cagcaccagg acctcggtcc
     2641 gcaactgcat ccagaggcgg cgcagccagt acatcagcgc tcttcctcct cgaagacgac
     2701 gccgtacagc ccttcgggcc gcagcagcag caccagcacc atgatcgaca acgcgaccgc
     2761 gcccttgagg aaagccccgg cgggcaccgc gaacacggtg atcgcctcga cgaagccgat
     2821 gatgaacgcc ccgacgaggc tgcccttgac gctgcccagg ccgccgagca ccacgatcgc
     2881 aagcatcgtc accagcggcc cgagccacat caacggatcc accacgaaca ccggcgccac
     2941 caccaccccg gcgatggccg ccagcgccac ggaaaacccg accgtcgcca gcgctacgcg
     3001 atcgacgttc atgcccatca ggttcgccac ttccaggtcg ttcgccgttg cgcggatggc
     3061 aagaccgagc cgcgttgcgt agagcaggta ccacgttccc gcaagcatca cgaaggcgac
     3121 caccagaatg acgaagcgct gatacgcgat gctcactccg gcgatccgca ccgtcccttc
     3181 gagggtcgac gggatgccca ggtaatggcc gccgaaggcc agcagcatca gttcctgaat
     3241 gatgatcgcc agggcaatgg tcgcgatcag caccgccgct tcatgctcgc gcaagggctc
     3301 gatgatcagc ttgtagcaga cgagcgaaag cgccgtgatt gcagccaccg cgatcacacc
     3361 cgcgagggcg aagttcagcc ccagcttgac cagcagggtg tagaagaggt acgcccccag
     3421 catgtagaac gcggtgtgcg cgacgttgac gatgcgcgcc acaccgaaga tcagcgaaaa
     3481 accgatggcg agcagcgcat acacgctgcc gttgacgagc ccggtgacga gaatgtcctg
     3541 gaacatcgca aaaagagccg cgcggcgtcg atccgcgcgg ctctcgggac cctttccccg
     3601 gccttacctg gccggcaaac ggaacggctg catgcccttg atcggcggcg gccagaacgc
     3661 gaccagcctt ccatcctgcc attgcacgcc gatgccgagc gacttgccga cgccccagac
     3721 cggatcatgg cgcttgtcga actcgagcac cgcgccgttg ccgacgtacg agatcttctc
     3781 cagcaccggt accatgtcgt ccgcgctggt cgagttcgcc ctttcgaccg cttccttgat
     3841 gagcatgatc gcgtcgtagg tcgcggcggt ataggtcggg gtcatcttga aacgctcgcg
     3901 aaaggcgcgg atccacggga tcgtcttggg cgtgatctcg acctcggcat aggtgttgag
     3961 cgtggacagg taattcgcct tgccggccgc ggcggcccag tactcatcct tctgcgcctc
     4021 gacgttgatg ccgaaggcga ccgccttcat gttgcgctcg cccatctggc ggccgaccac
     4081 gatgcccacc ggccccgaaa ggacggtgaa cacgatgtcg gcgccggcgc gctcgatcgc
     4141 cgagagctcg gcgttgacgt cggtggcgac cgccgaaggc tgccacaggc cgaccatttc
     4201 catcttcatc gccggcaggt tcttcttcgc ggcggcgatc aggccctcgg tccagaccgc
     4261 cttctcggcg agcaccgcga ccttcggcgt ctcggtcttc aatctcgtgc ggatttcgcc
     4321 cgccaccgcg ccgagcacgc cgaagacggt acgcccgagg tcggtgctct tggtgggcga
     4381 aacacggaac cagtacttgt agcggttgta gtccttttcg acccggaccc ccagttcgtc
     4441 atgcgcggcg ccgacgccga cgaacagctt cttgtggtcc atcgcgacct cctgcatcgc
     4501 cagcaccgcc tccgagcgga agccaccgat gaggaaatcg accttgtcgc gcgtgatgac
     4561 gcgctcgacc gcgctggcgg cgtcgggcac cgaggtcatc tcgttgctgt cggcccggat
     4621 cagctcgagc atcactttct tgccgccgac cgagatgccg cccgccttgt tgatctcgtc
     4681 gcgcgccatc tccgcgccca tccagtggtg ctggccctgc acgaacgcca tcggcccgag
     4741 aaccgcaatc ttgatcgggg cctgggcatg ggcggcagaa gcaagcgcaa gtgcggctgc
     4801 gaggacaaat ctattcagca tcggaacccc ctccatggtg gtcgagacct tccttcgccg
     4861 gcgatgttac cgcaagttcc gtgcagaccc tgcaggggtc aaaacacgat cacgcctctc
     4921 gcgttcttcc gggattcgag gtcggcaaag gcctgcggcg cctcgtcgat cgagtggcgg
     4981 cgcgtgatca gctcgtcgag cttgagcttg ccgcccatgt aaagctggag cagcctcggg
     5041 aagtcgacgc gcggcacgca ggagccgaag taactgcctt aaagatatga acttccttgc
     5101 gcgttgcgct gcacaaaacc ggccgcaaga ccagcgtgcc gcacacgttc taccggctcg
     5161 acgacggctc gcacctcgcc ttcttcgagg cgcccgacat gcccttcgag ttcaaggacc
     5221 agcacgactt cgacctgcac atcgcgctcg aggtggcgcg cggcgcgcgg cgtgctcgag
     5281 ccgatgatgg ccaagggaaa ggcgaacggc atcgaaacgc gcggcatctc gaaccacggc
     5341 ttcatcgact cgatctactt ttgcgatccg aacggctacg cgatcgaact gtgcgccaag
     5401 cgcgagcggc acgacgagga gatggacccg gcgcgcaacg gcgcgcgcgc gatcagcccg
     5461 gcccggggcg gttcgcggcg gcgtccttgc gccaggccgt cggcgccagg ccggtggcgc
     5521 gcttgaacgc ccgactgaag gcagccaccg actcgtagcc cacctcgagc gcgatcgacg
     5581 tcacgtccgc gtcggattgg cgcagcaaac ccgaggcgag ctgcatccgc cagcccgcga
     5641 ggtactgcat cggcgcctgg ccggtgaact gcacgaagcg ctcgtgcagc gccgagcgcg
     5701 acaggcccgt ttcgtcggcc agccggtcga tggtccaggc acggtcgggg tgcgcgtgca
     5761 tcagcgccag cgcgcggccc acgaagcgat cgcgcaaccc gcccagccag cctccctggg
     5821 tctcgggcag cctttcgagg tgcctgcgca ccaggtcgag caccatcatc tcgctcatgc
     5881 gctcgagcac cgccccgtcg cccgggcgca gctttccctc gcccgccccg cacgagcgga
     5941 cgaactgcgc cagccagcct tcgggcgagc cctggcccgc gcgctcgatc agcagccgcg
     6001 ggaggctcgc gatcagcggg ttgaagggcc gcacgtcgca gccgaggaag ccgcacacga
     6061 gcacggcgcg cccgcttccc ccgtccgcct cgggcgccac cagcgtgggc tggtcgccgt
     6121 gcacgtcgac gaagaacggg ggcccgatgc gcgccgggaa caggttcggg tcgaccgcct
     6181 gccgcgcgcg catgtgcggc gtgctcgaga gcacgtggcg atccccgtgc gggaacacga
     6241 cgatgtcgcc ttcgcgaacg agggctggct gctcgccgac gaccccgacc cagcacgacc
     6301 cgcgcgtgac cacgtgatac gccatcacgt gctcggcgcc cggaatcacc gccgaggcga
     6361 tctcgctggt ggggggcgac tccacgaccc acggcgcgcg cccctgcaga tgaaagaaca
     6421 ccgtgccgcg caatcgaatg ctgcgaagca cttccgagag cgtgtcctgt tccatgtcgg
     6481 gccggtccag tggtcgatgc ggagcgtggc cgaaatgcgg tcgcgaaacc ggcgaggcgc
     6541 cggcgatccg gccggctcgg gcgcacaacc ggattccggg tcaaccgcga cggacctgcg
     6601 gtgaaaaccc tgcgggctcg ccgtgcaacg atgctcccgt cgcgacgcca agccatttcg
     6661 cgtcgcgcgc agcccagcga atcctaccag aggggagaca cgagatgacg aacaccacga
     6721 ccaacgcagc ggcctcgccg gcgggcgcag cggcgcaacc cgatttcgcc gcgatcaagg
     6781 aacgacagaa ggcagtctgg aacgcgggcg actacgccgt gctggggggc acgctgcaga
     6841 tcgtgggcga actgctgtgc gaggccgtcg acctgcgcgc cggcgagaac gtgctcgacg
     6901 ttgccgcggg caacggcaac gcttcgctcg ccgcggcacg ccgctgggcg gcggtcacgt
     6961 cgaccgacta cgttgcgcag ctgctggaaa agggccgcgc gcgcgcggcg gccaacggca
     7021 tgccgatcga gttcaaggtc gcggacgccg agaacctgcc cttcgacgac gcgagcttcg
     7081 acgtcacgct gtcgaccttc ggggtgatgt ttgcgcccaa ccaggaccgc gccgccagcg
     7141 aactgctgcg cgtgacgcgt ccgggcgggc gcatcgggct ggcgaactgg acccccacga
     7201 gcctgatcgg agcgacgctc aagacggtgg gccggtacgt gccgccgccc ccggggctca
     7261 agccgccgct gctgtgggga accgagcccc gcatcgtcga gctcttcggg cccgctgccg
     7321 ccgacatcaa ggtgcagcgc agggacttcg tgttccgcta tcggtcggcg cggcactggc
     7381 tcgacgtgtt caccgcgtac tacgggcccc tgctcaaggc gatcgagtcg ctcgacgaga
     7441 gcgggcgcgc ggcgctgctc ggcgagctcg aggcgctgct cgggcagtcg aaccgctccg
     7501 gctccgattc gctggtggcg ccgtcggagt atctcgagat cgtgatcacc agggcctgag
     7561 gcgaaagata ggaacttcct tccgcggcgc gctgcgcaaa aatctggcag tgcacgcccg
     7621 cacgacggtg gtacagggcg ccggtactcc acacggcggg cctcggcgca gcccatcaag
     7681 gcccatcgat gccgactccc actcgatcgg ccgatcggcg cgaaccagac cggattgggg
     7741 aatccctcgc gcgtcgaggg tttctgcaat tagcgatcac cttcgggccg aagtagcgta
     7801 agctgcatcg gcgtgcccgt tctgggagga gccggcgcga ccccgccgca acggatttcg
     7861 ctttccagtc caacgaggaa ctcgccgacg gttccgtggg cgagatccct tcaccgcctg
     7921 atgttgcggg tcaaccattt cacctcatgg aggcgagttt catgccgaac gaagccatgc
     7981 aaaagctcaa gggcaacatc aagggccgcg tcattctccc ggaagaccgg aactatgacg
     8041 acgtcaggaa aatctggaat gcgatgatcg accgccgtcc cgcggtcatc gtgcaatgcg
     8101 cgagcccggg cgatgcgcgg catgcgattc aatatgcgcg cgaccagggc ctcgacatcg
     8161 ccatccgcgg cgcggggcac aacatcgccg gcaacggcgt ctgcgaaggc ggcctgctga
     8221 tcgatcactc gaccatgaaa ggcgtgcgcg tggatgcggc caagcggcgg gcctatgtgg
     8281 agcccggcgc gacgctgggc gacttcgatg ccgcggtgca ggcacacggc ctggcgacgc
     8341 cggtgggcat caattcgacg accgggatcg ccggcctcac gctgggcggc ggtttcggct
     8401 ggctgacgcg caagcacggc atgacgatcg acaacctcgt ggcggcgaat gtcacgctcg
     8461 cgaacggcaa agaggtgcgg gccagcgccg atgaacatgc cgatctcttt tgggccattc
     8521 gcgggggagg cggcaatttc ggcgtcgtca cgcaattcga gttcgcgctc catcccgtcg
     8581 gccccgaagt cttcgccggg ctgatggtgt ttccgggcgc acaggcgaag tcggtgctga
     8641 agaaatatcg cgacttcgtc gcctcggcgc cggaggaact ctgcgtctgg gtcgtgcttc
     8701 gcaaggcgcc gccgctgccc tttctgcccg agaacgtgca tggcaaggac gtggtcgtgc
     8761 ttgccctgtg ctatgccggc gacgtcgccc agggcgagaa gctgatcgag ccgatgcgcg
     8821 ggtttggcga cgcctatggc acgcacgtgg gcgccatgcc ctatgcaggc tggcagcagg
     8881 cgttcgaccc gctgctcacg ccgggcgcgc ggaactactg gaagtcgcac aatctgcagt
     8941 cgctcggcga cgcggcggtg gattcgatcc tcgcgtacgc cggcaagctc ccgtcggcgc
     9001 agtgcgaaat cttcatcgcg cacattgccg gcgcggccaa tcgcgtgcgg gccgacgcca
     9061 tggcgtacgg acaccgcgac gcgaagttcg tgctgaacgt tcacggacgg tgggacgcgg
     9121 cggccgacga taaggcctgc atcggatggg cgcgcgagtt ctttgcggcg tccgcgccgt
     9181 acgcatctgc cggggcgtat atcaatttca tgacggagga cgaaaccgac cgcgtgcagg
     9241 ccgcattcgg cgcaaatcac gcgcggctgg cgacgatcaa gaaacagtac gatccggaaa
     9301 acatcttcca cctgaaccaa aacatcaagc cgtgaccgtg ccgtcgaacg ccatcccgag
     9361 ggggcggcgg tagcgcttgg gtcggggccc ccgtttgcgg ggccccaaac gcgtgcctcg
     9421 cgagcagtcg cttcccggag gcccgagcga acaccccgct cggcggagcg ccccgggtcg
     9481 cgtcacgcgt ccggcgcgcg gggcgcgcct gggctacttt tgcctatcgg ctttccggat
     9541 cgctccatgc cgggccagag cagcgccctc gctcccctgg ccgtcgtcac cggcggcgcc
     9601 cgcggtatcg gccttgcgat cgccgagcgt ttccttgccg aagggcaccg cgtcgcgctg
     9661 ctggacatcg accgcgggac gctgcgcgcg accgaaaagc gcctgaacaa ccaaagatag
     9721 gaacttcctt cctcgttggg ctgcgcaact ggcgtggctc gcggcggcga ggccgcgatt
     9781 cggggctcgg gggctgaagg ttcgagcggc gcgcaggcaa ggcgccgcgg tagcggcgg
//
LOCUS       DBNHKE010000021         7811 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_691026, whole genome shotgun sequence.
ACCESSION   DBNHKE010000021 DBNHKE010000000
VERSION     DBNHKE010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_691026"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="AC16O9_01275"
     CDS             complement(<1..1866)
                     /locus_tag="AC16O9_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6515281.1"
                     /translation="MPWQFWIDRGGTFTDIVARRPDGALVAHKLLSENPERYKDAALA
                     GIRELLGLAPGARIPGKDIDAVKMGTTVATNALLERKGDRTVLFITKGFRDALRIAYQ
                     NRPKIFERNIVLPELLYADVLEVQERIGARGEVLIPLDESRARADLEKSFKAGFRSIA
                     IVFMHGYRYTQHEARVAALAKEVGFTQISVSHLTSSLMKLIGRGDTTVVDAYLSPILR
                     RYVEQVAAELEGVRLLFMQSNGGLTDARRFFGKDSILSGPAGGIVGAVRTSLAAGFNK
                     IIGFDMGGTSTDVSHYAGEFEREFETQVAGVRMRAPMMSIHTVAAGGGSILHFDGARY
                     RVGPDSAGANPGPASYRRGGPLAVTDANVMLGKIQPGYFPRVFGAGGDEALDAAVVKQ
                     KFVELAQTIGDGRAPEEVAEGFVKIAVGNMANAIKHISVQRGHDVTEYTLCCFGGAAG
                     QHACLVADALAMTRVFIHPLAGVLSAYGMGLADQVEMREKAVEAKLGSAALVEDLDVL
                     AKAAAEELVAQGVERGRIRVIRRVHLKYEGTDTALIVPFGSLEDMTAAFEAAYRKQFS
                     FLMRGKQLIVEAVSVEALSAGETFRPFSEEHGKGAIDMKPEARVKMFTGGRWHETP"
     gene            complement(1866..2618)
                     /gene="cnbZ"
                     /locus_tag="AC16O9_01280"
     CDS             complement(1866..2618)
                     /gene="cnbZ"
                     /locus_tag="AC16O9_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-5-chloromuconate deaminase CnbZ"
                     /protein_id="HIR6515282.1"
                     /translation="MSALPVFREGGYRFIKAVFQYSGGVAAEPGFEIVRARLATVLPL
                     AQGFKTVEAYLKAAGRPLTAFCACELRSPAPFTEEGFLAFNKIYASTLERWGIYKDGA
                     NPVARTNVCPVFAPPAEPSLYAFSYTVPAAKGARGSFILAGGGEAPDGKPSYHDFIVR
                     RGDTSPEGLREKVRYVMAEMERRLAALGFSWKDAVSTQAYTVHDIGALVQKEIVERGA
                     AAGGLEWHLARPPIVGLDYEMDVRGAAREVVL"
     gene            complement(2620..3867)
                     /locus_tag="AC16O9_01285"
     CDS             complement(2620..3867)
                     /locus_tag="AC16O9_01285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6515283.1"
                     /translation="MARYDIVVLGAGIVGLCVALHLQKRGRAVALVDRRGAAEETSFG
                     NAGLIQREGVFPYGFPHDFGALARYALNRTVDAHYHPSALPALAPFLWKYWHHSRPAR
                     HAEVARRYARLIEHCVAEHDALVAEAGAEDLIVRGGWMKVFRTERERDRRFAEAEGWK
                     MDFGVEHRKLDARALQEAEPHLAPVLAGAIHWTQPTRALDPHALGQAYLKRFQDLGGV
                     FVQGNATTLEKAGDGWKLRTAKGALEAGGAVVALGPWSDVLTRALGYELPLAVKRGYH
                     MHYRAAGGAVLNHPILDVERGYFMGPMQRGVRLTTGAEFALRDAPRTPVQLARAEPIA
                     RDLFPLAERLDAEPWMGARPCTPDMLPVIGPAPRHDKLWFAFGHAHHGLTLAAVTGRL
                     LAEMVTGEAPFVDPAPYAAARFG"
     gene            3885..4982
                     /locus_tag="AC16O9_01290"
     CDS             3885..4982
                     /locus_tag="AC16O9_01290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6515284.1"
                     /translation="MDRIDTAVIGAGVVGLAVARALAAQGREVVILEAEDAIGTHTSS
                     RNSEVIHAGIYYPPGSLKARACVAGRERLYAYCAERGVPHRRCGKLIVATDAAQRDAL
                     EGIRRKAHANGVTDVVRVSPAEVRAMEPELACVDALYSPSTGIIDSHALMLAYLGDAE
                     NAGAMLALKSPLEAARVTPQGIELHVAGAEPLLARHVVNSAGLTAPSLARRFEGYAPQ
                     HAPRELYAKGNYYSLARRAPFTHLVYPVPEPGGLGVHVTLDLGGRARFGPDVEWVDSI
                     DYRVDPARAQAFYAAIRRYWPGLPDGALEPGYAGIRPKISGRGEPAADFVVQGPHAHG
                     VPGLVHLFGIESPGLTASLALADDAAAQLSD"
     gene            5000..5968
                     /locus_tag="AC16O9_01295"
     CDS             5000..5968
                     /locus_tag="AC16O9_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6515285.1"
                     /translation="MKFRLAAAVAAAVACSAAQAQTKWDMPTPYSDGEFHTRNVVLFV
                     EDVAKRTNGALEIKVHSSGSLIKHPDILRAVSTGQVNIAEFLLGQFGNEDPVFAADNL
                     PFVAAGYDSAQKFYAAQKPLLTKKLAERGVTLLYSVPWPGQGIYTKEPLKSVDDLKGT
                     KFRTYSPLTARLAELLGASPTVIQVPEIPQMFATGAVQAMITSSATGTSTKAWEFVKN
                     YYKTSAFHPKNVVVVNTRALKRLPKAQQDALLGAAQDAEPRGWAMSKQRERDGDETLA
                     KNGMTLHEPSAAMKAAFEKVGAQMAKEWEDKAGADGQSIMKAYHKK"
     gene            5970..6503
                     /locus_tag="AC16O9_01300"
     CDS             5970..6503
                     /locus_tag="AC16O9_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6515286.1"
                     /translation="MRRFLDRLYTASGVLAAACLASIAVVMLAQAGMREMGMLLRGAD
                     DIVGWLCAASAFLALGYTFRHGELVRVGLFIDRLPPASRRRAEFAALGLGVLVGGYIL
                     WAAANFVYESWQFEEVAQGLIQIPIWIPQMSFVVGALIFFIALLDEFIVLAMGGTPAY
                     RAAEETHRAHPEAAEAP"
     gene            6503..>7811
                     /locus_tag="AC16O9_01305"
     CDS             6503..>7811
                     /locus_tag="AC16O9_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6515287.1"
                     /translation="MGTLTVAAILLAVLLVLLAGGLWIAMAMSVVAWVGLQFFTTSPP
                     DVNLFQAYWGSSASWTLAALPMFVWMGEILFRTKLSEEMFKGLSPWLGWLPGRLMHVN
                     IFGCAIFAAVCGSSAATVATIGKVALPELAKRGYDETTSVGSLCGAGTLGLLIPPSII
                     MIVYAVAAEVSILKMFLAGVFPGALLVSLFSGYIITWALLNPGKTPTAVEKMSLGERV
                     RRAKNLIPCLALIFFIMGVMFTGVATATEAAAFGVLGALAIAWWSGSLTRQSFVTSLM
                     GATRLSCMILFILAGASMLSSCMAFTGIPRALAEWVAASNFSPYALIAVLALIYLLLG
                     MAIDGISMIVLTTSIVLPMIDAAGFDLIWFGIFIVLLVEIAQITPPVGFNLFVLQTMA
                     KREANYVARAAIPFFFMMLLAIVVVTAFPAIATWLPDAILGVTK"
BASE COUNT         1193 a         2794 c         2598 g         1226 t
ORIGIN      
        1 gggcgtctcg tgccaccttc cgcccgtaaa catcttcacc cgggcctcgg gtttcatatc
       61 gatcgcccct tttccgtgct cttcggaaaa ggggcgaaag gtttcccccg cgctcaacgc
      121 ctccaccgac accgcttcga cgatgagctg cttccctctc atcaggaacg aaaactgttt
      181 ccgataggcc gcctcgaaag cggccgtcat gtcctcaagc gacccgaacg gcacgatcag
      241 cgccgtatcc gtgccttcgt acttcaggtg cacccgccgg atgacgcgga tgcgcccgcg
      301 ctcgacgcct tgcgcgacga gctcctccgc cgccgctttc gccagcacgt cgagatcctc
      361 caccagcgcg gcggacccca gcttcgcctc caccgccttt tcccgcatct ccacctgatc
      421 cgcgagcccc atgccgtagg ccgagagcac gcccgcgagc gggtggatga acacgcgcgt
      481 catggcgagc gcgtcggcga cgaggcaggc gtgctgtccc gccgcgccgc caaagcaaca
      541 caacgtgtac tcggtgacat catggccgcg ctgcaccgag atgtgcttga tggcgttcgc
      601 catgttgccg accgcgatct tcacgaatcc ttccgccacc tcctcgggcg cgcgcccgtc
      661 gccgatggtc tgcgcgagct cgacgaactt ctgcttcacc accgccgcgt cgagcgcctc
      721 gtcgcctccg gcgccgaaca cgcgcggaaa atagcccggc tggatcttgc ccagcatcac
      781 gttggcgtcg gtcaccgcga gcggcccgcc gcgccggtag ctcgccgggc cggggttcgc
      841 gcccgccgag tccggcccga cgcggtagcg cgcgccgtcg aagtgcagga tcgagccgcc
      901 gcccgcggcc accgtgtgga tgctcatcat cggcgcgcgc atgcgcaccc ccgccacctg
      961 ggtctcgaac tcgcgctcga actcgcccgc gtagtgcgac acgtcggtcg aggtgccgcc
     1021 catgtcgaag ccgatgatct tgttgaaccc cgcggcgagc gaggtgcgca ccgcgccgac
     1081 gatgccgccc gccggcccgg agaggatcga gtccttgccg aagaagcgcc gcgcatcggt
     1141 gagaccgccg ttcgactgca tgaacaggag ccgcacgcct tccagctcgg cggccacctg
     1201 ctcgacgtag cggcgcagga tcggcgacaa gtaggcgtcg actacggtgg tgtcgccgcg
     1261 cccgatcagc ttcatcagcg agctggtgag gtgcgaaacg gagatctggg taaacccgac
     1321 ctccttggcc agcgcagcca cgcgagcttc atgctgcgta taccgatacc cgtgcatgaa
     1381 aacgatcgcg atcgaccgaa accccgcctt gaagctcttc tcgagatcgg cgcgcgctcg
     1441 cgactcgtcc agcgggatca ggacttcgcc gcgcgcgccg atcctttcct gaacctcgag
     1501 cacgtccgca tacaacaact ccgggaggac aatgttgcgc tcgaagatct tcggcctgtt
     1561 ctgatacgcg atgcggagtg cgtccctgaa ccccttcgta atgaacagaa ccgtccgatc
     1621 ccccttgcgc tcgagcaaag cattcgtcgc gacggtggtg cccatcttca ccgcgtcgat
     1681 gtccttgccc gggatgcgcg cccccggcgc caggccgagg agctcgcgaa tcccggcgag
     1741 cgccgcgtcc ttgtagcgct ccgggttctc cgaaagcagc ttgtgcgcga ccagcgcgcc
     1801 gtcggggcgg cgcgcgacga tgtcggtgaa cgtgccgccg cggtcgatcc agaactgcca
     1861 gggcatcaga gcacgacctc gcgcgcggca ccgcgcacgt ccatctcgta gtcgagcccg
     1921 acgatcggcg ggcgcgcgag gtgccattcc agcccgcccg ccgccgcgcc gcgctcgacg
     1981 atctcctttt gcaccagcgc cccgatgtcg tgaacggtgt aggcctgcgt ggaaacggcg
     2041 tccttccagg agaagccgag cgcggcgagg cgccgctcca tctcggccat cacgtagcgc
     2101 accttctcgc gcaggccctc gggcgaggtg tcgccgcgac gcacgatgaa gtcatggtaa
     2161 ctgggcttgc cgtccggcgc ctcgccgccg ccggcgagga tgaagctgcc gcgcgcgccc
     2221 ttggcggcag gcaccgtata ggagaaggcg tacagcgaag gctcggccgg cggcgcgaag
     2281 accgggcaga cgttggtgcg cgccaccgga ttggcgccgt ccttgtagat gccccagcgc
     2341 tccagcgttg aggcgtaaat cttattgaac gcgagaaagc cctcctcagt gaacggcgcc
     2401 ggcgagcgca gctcgcacgc gcagaacgcc gtgagcggcc ggcccgccgc cttaaggtag
     2461 gcctcaaccg tcttgaaccc ctgcgccagc gggagcaccg tggcgagccg agcgcgcacg
     2521 atctcgaagc cgggctccgc ggcgacgccg cccgaatact ggaacaccgc cttgatgaag
     2581 cggtagcccc cttcgcgaaa caccggtagc gcgctcatgt cagccgaatc ttgccgcagc
     2641 atacggcgcc ggatcgacaa acggcgcctc ccccgtcacc atttcggcca gcagccgccc
     2701 ggtgaccgcc gcgagcgtga gcccgtggtg cgcgtggccg aaggcgaacc agagcttgtc
     2761 atgtcttggc gccggcccga tcaccggcag catgtccggc gtgcacggcc gcgcgcccat
     2821 ccacggctcg gcgtcgagcc gctcggcgag cgggaacagg tcgcgcgcga tcggctcggc
     2881 gcgcgccagc tgcaccggcg tgcgcggcgc atcgcgcagc gcgaactccg cgcccgtggt
     2941 gaggcgcaca ccgcgctgca tcggccccat gaagtagccg cgctcgacgt ccaggatcgg
     3001 gtggttgagc accgcgcccc ctgccgcgcg gtagtgcatg tggtagccgc gcttcaccgc
     3061 gagcggcagc tcgtagccga gcgcgcgcgt cagcacgtcg gaccacggcc cgagcgcgac
     3121 caccgcgccg ccggcctcga gcgcgccctt ggcggtgcgc agcttccagc cgtcgcccgc
     3181 cttctccagc gtcgtggcgt tgccctgcac gaacacgccg ccgaggtcct ggaagcgctt
     3241 caaataggcc tgcccgagcg cgtgcgggtc gagcgcgcgc gtgggctgtg tccagtggat
     3301 cgcgcccgcg agcaccggcg cgaggtgcgg ctcggcttcc tgcagggcgc gcgcgtcgag
     3361 cttgcggtgc tcgacgccga agtccatctt ccagccttcg gcctcggcga agcgccggtc
     3421 gcgctcgcgc tcggtgcgaa acaccttcat ccagccgccg cgcacgatca ggtcctcggc
     3481 gccggcctcc gccaccagcg cgtcgtgctc ggcgacgcag tgctcgatca accttgcata
     3541 gcggcgtgcc acctcggcat ggcgcgccgg acgcgagtgg tgccagtact tccacaggaa
     3601 cggcgcgagc gcgggcagcg ccgacggatg gtagtgcgcg tcgacggtgc gattgagcgc
     3661 gtagcgtgcg agcgcgccga agtcgtgcgg gaagccgtac gggaagacgc cctcgcgctg
     3721 gatcaggccg gcgttgccaa acgaggtctc ctcggccgcg ccgcgccggt cgacgagcgc
     3781 caccgcgcgg ccgcgcttct gcaggtgcag cgcgacgcac aggccgacga tgccggcgcc
     3841 caggacgacg atgtcgtagc gtgccattag aatcgcatcc taacttggat cgcatcgata
     3901 ccgcagtcat cggcgcaggg gtggtcggcc tcgcggtcgc gcgcgcgctc gccgcgcagg
     3961 ggcgcgaggt ggtgatcctc gaggccgagg atgccatcgg cacgcacacc agctcgcgca
     4021 attccgaggt gatccacgcc ggcatctact atccgccggg ctcgctcaag gcgcgcgcct
     4081 gcgtcgccgg gcgcgagcgc ctgtacgcct actgtgccga gcgcggcgtg ccgcaccggc
     4141 gctgcggcaa gctgatcgtt gccaccgatg ccgcgcagcg cgacgcgctg gaaggcatcc
     4201 ggcgcaaggc gcacgccaac ggcgtcaccg acgtggtgcg cgtcagcccc gccgaggtgc
     4261 gcgccatgga gcccgagctc gcctgcgtgg acgccctcta ctcgccctcg accgggatca
     4321 tcgacagcca cgcgctgatg ctcgcctacc ttggcgacgc cgagaacgcc ggcgccatgc
     4381 tggcgctgaa gagcccgctc gaggccgcac gcgtcacgcc ccagggcatc gagctccacg
     4441 tggcgggcgc cgagccgctg ctcgcccgcc atgtcgtcaa cagcgccggg ctcacggcac
     4501 cgagcctcgc gcggcgcttc gagggctatg cgccgcagca tgcgccgcgc gagctctacg
     4561 ccaagggcaa ctactacagc ctcgcgcgcc gcgcgccgtt cacgcacctg gtctacccgg
     4621 tgcctgagcc ggggggcctg ggcgtgcacg tcacactcga tctgggcggg cgcgcgcgct
     4681 tcgggccgga cgtggaatgg gtggacagca tcgactaccg cgtcgacccg gcgcgcgcgc
     4741 aagcgttcta cgccgccatc cgacgctact ggccgggact gcccgacggc gcgctcgaac
     4801 cgggctacgc cggcatccgc ccgaagatct ccggccgcgg cgagccggcc gcggacttcg
     4861 tcgtgcaggg cccgcacgcg cacggcgtgc cggggctggt gcaccttttc ggcatcgagt
     4921 ctcccggatt gacagcttcg ctcgcgttgg ccgatgatgc ggcagcgcaa ctttctgact
     4981 gaaacttccc ggaggtgaca tgaaattcag gctcgcggca gcggtcgcgg cggcggtggc
     5041 atgcagcgcc gcgcaagcgc aaaccaagtg ggacatgccc acgccctact cggacggcga
     5101 attccacacg cggaacgtcg tactcttcgt cgaggacgtc gccaagcgca ccaacggcgc
     5161 gctcgagatc aaggtgcact cttcgggctc gctcatcaag caccccgaca tcctgcgcgc
     5221 ggtgtccacc ggccaggtca acatcgccga attcctgctc gggcagttcg gcaacgagga
     5281 cccggtgttc gccgccgaca acctgccgtt cgtggctgcg ggctacgaca gcgcgcagaa
     5341 gttctacgcg gcgcaaaagc cgctgctcac gaagaagctc gccgagcgcg gcgtgacgct
     5401 gctgtactcg gtgccgtggc cgggccaggg catctacacc aaggagccgc tgaagtcggt
     5461 cgacgacctg aagggcacca agttccgcac ctactcgccg ctcaccgcgc gcctggccga
     5521 gctcctgggc gccagcccga ccgtgatcca ggtgcccgag atcccgcaga tgttcgccac
     5581 cggcgcggtg caggcgatga tcacctcgtc ggcgaccggc acctcgacca aggcctggga
     5641 gttcgtcaag aactactaca agacgagcgc cttccatccg aagaacgtgg tggtggtcaa
     5701 cacgcgcgcc ctgaagcgcc tgcccaaggc gcagcaggac gcgctgctcg gcgcggcgca
     5761 ggacgccgag ccgcgcggct gggcgatgtc caagcagcgc gagcgcgacg gcgacgagac
     5821 gctggccaag aacggcatga cgctgcacga gccgagcgcc gcgatgaaag ccgcgttcga
     5881 gaaggtcggc gcgcagatgg cgaaggaatg ggaagacaag gccggcgccg acggccagtc
     5941 gatcatgaag gcctaccata agaagtaatt tgaggcggtt tctcgaccgc ctctacaccg
     6001 cgagcggggt gctcgcggcg gcctgcctgg cgagcatcgc cgtggtgatg ctcgcgcagg
     6061 ccggcatgcg cgaaatgggc atgctgctgc gcggcgccga cgacatcgtc ggctggctgt
     6121 gcgcggcgag cgcgttcctc gccctcggct ataccttccg ccacggcgag ctggtccggg
     6181 tcgggctgtt catcgatcgg ctgccgccgg cgagccggcg ccgcgctgaa ttcgcagcgc
     6241 tcggcctcgg tgtgctggtc ggcggctata tcctgtgggc ggcggcgaac tttgtctatg
     6301 aaagctggca attcgaggag gtggcacagg ggctgatcca gattccgatc tggatcccgc
     6361 agatgagctt cgtcgtcggc gcgctcatct tcttcatcgc gcttctcgat gagttcatcg
     6421 tgctggcaat gggcggcacg ccggcgtatc gcgccgccga ggaaacgcac cgcgcgcacc
     6481 ccgaggccgc cgaggcgccc tgatgggcac gctcaccgtc gccgccatcc tgctcgccgt
     6541 cctgctcgtg ctgctcgcgg gcgggctgtg gatcgcgatg gcgatgagcg tggtggcctg
     6601 ggtcgggctg cagttcttca ccaccagccc gcccgacgtc aacctgttcc aggcctactg
     6661 gggctcgagc gcgtcgtgga cgctcgccgc gctgccgatg ttcgtgtgga tgggcgagat
     6721 cctgttccgc acgaagctct cggaagagat gttcaagggc ctgtcgccgt ggctcggctg
     6781 gctccccggg cggctgatgc acgtcaacat cttcggctgc gccatcttcg cggcggtgtg
     6841 cggctcgtcc gcggccacgg tcgccaccat cggcaaggtg gcgctgcccg agctcgccaa
     6901 gcgcggctac gacgagacaa cgagcgtggg ttcgctgtgc ggcgccggca cgctcggcct
     6961 gctgattccg ccctcgatca tcatgatcgt gtacgcggtg gcggccgagg tgtcgatcct
     7021 gaagatgttc ctcgccggcg tgttcccggg cgcgctgctg gtgagcctgt tctccggcta
     7081 catcatcacc tgggcgctgc tcaatccggg caagacgccc accgccgtcg agaagatgag
     7141 cctcggcgag cgcgtgcgcc gcgcgaagaa cctgattccg tgcctcgcgc tgatcttctt
     7201 catcatgggc gtgatgttca cgggcgtcgc cacggccacc gaagccgcgg cgttcggcgt
     7261 gctcggtgcg ctggcgatcg cgtggtggag cggctcgctc acgcgccaga gcttcgtcac
     7321 cagcctgatg ggcgcgacgc gcctgtcgtg catgattctg ttcatcctcg ccggcgcctc
     7381 gatgctctct tcgtgcatgg cgttcaccgg catcccgcgc gcgctcgccg agtgggtggc
     7441 ggcctcgaac ttcagcccct atgcactgat cgcggtgctg gcgctcatct acctgctgct
     7501 cggcatggca atcgacggca tctcgatgat cgtgctcacc acctcgatcg tgctgccgat
     7561 gatcgacgcc gcgggcttcg acctgatctg gttcggcatc ttcatcgtgc tgctggtcga
     7621 gatcgcgcag atcacgccgc cggtcggctt caacctgttc gtgctgcaga ccatggccaa
     7681 gcgcgaggcg aactacgtgg cgcgcgccgc gatcccgttc ttcttcatga tgctgctcgc
     7741 gatcgtggtg gtcaccgcgt tcccggcgat cgccacctgg ctccccgacg ccatcctcgg
     7801 ggtgacgaaa a
//
LOCUS       DBNHKE010000022         2088 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_214702, whole genome shotgun sequence.
ACCESSION   DBNHKE010000022 DBNHKE010000000
VERSION     DBNHKE010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_214702"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1076
                     /locus_tag="AC16O9_01310"
     CDS             276..1076
                     /locus_tag="AC16O9_01310"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR6515288.1"
                     /translation="MSTARTYDPEPAARLLIGPWRENAKFDMLPDAVRPTTRAEGYRV
                     QAALVAASGEDAVGWKLAATSAAGQKHIAVTMPLAGRLLRSRQRAEGSAIPVRGLGMA
                     VAEPEFAFRMARDLRAPAHAGAFSREAVMDAVADLHLAIEFPDSRFRDFAVAGEAQLI
                     ADFACACFFVLGAKADASWRALDLAEHVVRVRVDAQEVAQGKGANVLGDPRTALTWLA
                     NELRTQGAFLRAGDIVMTGTCVTPVPIRAGQKLEADFGALGKVSAATV"
     gene            1119..1559
                     /locus_tag="AC16O9_01315"
     CDS             1119..1559
                     /locus_tag="AC16O9_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6515289.1"
                     /translation="MRLPTLDDAIEASHRALQAIIRGDVEPFMALYSDANDITIGNPF
                     GPFAAGHDATRAAGTRAASNYTDGEILAFESIATHATDTLACVVEVERFSVRLAKAAE
                     LATVALRVTSLYRREAQGWRLVHRHADPITGVRPAQSVASSERG"
BASE COUNT          344 a          680 c          740 g          324 t
ORIGIN      
        1 ataggaactt ccttcctcgt tgggctgcgc aactggcgtg gctcgcggcg gcgaggccgc
       61 gattcggggc tcgggggctg aaggttcgag cggcgcgcag gcaaggcgcc gcggtagcgg
      121 cggggcaccg gcgttatggt aaataacccg gagtatcccg acttcccaag aagcggacgc
      181 tcggcggcgg ggtattatcc acgaggggcc cggctcggcc ggaagatggc gaagtgagcg
      241 gccggcatct ccccctgagt catggaagga cgatggtgag cacagccaga acctacgatc
      301 ccgagcccgc ggcccggctg ctgatcggcc cgtggcgcga aaacgccaag ttcgacatgc
      361 tgcccgatgc cgttcggccg accacgcgag ccgagggcta ccgcgtgcag gcggcgctcg
      421 tcgccgcgtc tggcgaggac gcggtgggct ggaagctcgc cgccaccagt gccgccgggc
      481 aaaaacacat cgccgtcacg atgccgctcg ccggccgcct gttacgcagc cgccagcgcg
      541 cggagggcag cgcgatcccc gttcgcggcc tgggcatggc ggtggcggag ccggagttcg
      601 cgttccgcat ggcgagggat ctgcgcgccc cggcgcacgc cggcgcgttc tcgcgggagg
      661 cggtgatgga cgcggtggcg gatctgcatc tggcgatcga gtttcccgat tcacgcttcc
      721 gggatttcgc ggtcgccggc gaggcgcagc tgatcgcgga cttcgcctgc gcctgcttct
      781 tcgtgctggg cgccaaggcc gacgcatcgt ggcgcgcgct cgacctcgcc gagcacgtcg
      841 ttcgcgtccg cgtcgacgcg caggaggtgg cgcagggcaa gggtgccaac gtgctgggcg
      901 acccgcgcac ggcgctcacc tggctcgcca acgagctgcg cacgcagggc gcgttcctgc
      961 gcgcggggga catcgtcatg accggcacct gcgtgacgcc cgtgccgatc cgtgccggac
     1021 agaagctcga ggccgacttc ggcgcgctgg gcaaggtgtc cgccgcgacc gtgtgacgca
     1081 cgctcgccgc aatcctgcat tcacccatgg gagcgggcat gagactgcca acgcttgacg
     1141 acgccatcga ggcgagccac cgtgcgcttc aggccatcat ccgaggcgac gtcgaaccat
     1201 tcatggcctt gtactcggat gcgaacgaca tcacgatcgg caatcccttc ggaccgtttg
     1261 ccgccgggca cgacgccacc agagcagccg gtacccgagc tgccagcaac tacacggacg
     1321 gcgagatcct ggcgttcgaa agtattgcta cccacgccac cgacactcta gcctgcgtcg
     1381 ttgaggtcga acggttttcg gtacggctgg caaaggcggc cgagctcgct acggtcgcgc
     1441 tacgggtcac cagcctgtac cggcgcgaag cgcaaggctg gagactcgtc caccggcatg
     1501 ccgaccccat caccggcgtc cgaccggcgc aatccgttgc cagttcagag cggggttgac
     1561 ttggcggaat gaatgtccgt cgcttaggca caggcggcgc acttcagggg cagcgtacaa
     1621 aagatgggaa cttccttccg cgttgcgtcg tgcaacaggc gcggcgcagg ccgctgcgcg
     1681 ttcccgagag cgcaagagct atggcagaac gctcggacag gacaggtgta cggggcggac
     1741 gtgcacatct atgcctagca ggcgacgcgc cgcggcaagg ccctccgcct aagcggcgca
     1801 actcttgggc gctcgacctg gatcaagcct cagcatttct ctagcggctg gatgtcgctg
     1861 gacagccttg gtccgctttg tacaggggaa atcccggcca gccagctcgg gccagggatt
     1921 ccactggggg tattggtgga ggctctatac ctaaaactgg gaaagatagg aacttccttc
     1981 ctcgttgggc tgcgcaactg gcgtggctcg cggcggcgag gccgcgattc ggggctcggg
     2041 ggctgaaggt tcgagcggcg cgcaggcaag gcgccgcggt agcggcgg
//
LOCUS       DBNHKE010000023        10020 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1325735, whole genome shotgun sequence.
ACCESSION   DBNHKE010000023 DBNHKE010000000
VERSION     DBNHKE010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1325735"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1875
                     /locus_tag="AC16O9_01320"
     CDS             <1..1875
                     /locus_tag="AC16O9_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6515290.1"
                     /translation="ISHGITKTPQPHAGKAEQEAGDEAQAEPSNSPLDNYTQNLNALA
                     LAGKIDPLIGRERELERVIQTLCRRRKNNPLLVGEAGVGKTAIAEGLARRIIESNVPE
                     LLARSTVYALDMGALLAGTKYRGDFEQRLKAVLKQLVDNPNAILFIDEIHTVIGAGAA
                     SGGTLDASNLLKPVLSTGQLKCIGATTYNEYRGVFEKDHALSRRFQKIDVVEPSVDET
                     VEILKGLKTRFEAHHSVKYTATALSSAAELSARYINDRHLPDKAIDVIDEAGAAQRIA
                     PKSRQKRVIGKGEIEEIVAKIARIPPRSVSSDDRGALAKLDRDLKAVVFGQDQAIDAL
                     ASAIKMARSGLGNPQKPIGSFLFSGPTGVGKTEVARQLAYCMGIELIRFDMSEYMERH
                     AVSRLIGAPPGYVGFEQGGLLTEAITKKPYAVLLLDEIEKAHPDIFNILLQVMDHGTL
                     TDNNGRKADFRNVVIVMTTNAGAESLTKNAMGFTPSVKAGDEMEAIKKMFTPEFRNRL
                     DAVISFRALDHDVIMRVVDKFLIQLEEQLQEKKVEPTFTPKLKEHLAKKGFDPLMGAR
                     PMARLIQDTIRRALADELLFGKLAHGGKVTVDIDDAGNVALQFEAEAPAPPPAKVPVT
                     "
     gene            2071..2313
                     /locus_tag="AC16O9_01325"
     CDS             2071..2313
                     /locus_tag="AC16O9_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515291.1"
                     /translation="MSAQDAGSRLSASCAMCHGTAGHSVGGNAPLAGMPRDDLVRAMK
                     EFQSGARPATAMHQIAKGYSDREIEQIAAYFAAQKK"
     gene            2323..3594
                     /locus_tag="AC16O9_01330"
     CDS             2323..3594
                     /locus_tag="AC16O9_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6515292.1"
                     /translation="MAKTFDRRNFLKLSGAAAALAATGCATSGGAKARVVVIGGGFGG
                     ATAAKYIRMWGPAIEVVMVERNTDFVSCPISNLVFGGSKTMADITYGYSGLSKYGVQV
                     VRDEVTAVDTGAKSVRLARGGELKYDRLVVSPGIDFMYEQIAGYEAAMDSSRVLHAWK
                     AGPQTVALQRQLAEMRLGGVYVLAVPLAPYRCPPGPYERASQVAQYFKKHNPRAKVLI
                     VDANPDVTSKGKLFKQAWADLYPGMIEFRGNSKAIGVDPGTGTVKTEVEEVKGDVLNV
                     VPAQRAGTIAAKAGLITANDRWCGVDWRTTESTVAKGVHVLGDATLSAPGMPKSGSMA
                     NQQAKVCAAAVVAMINGSEPNPNPKIANMCYSFVSDDEVIHVTSVHELDAGKKTFVPI
                     KGAGGLSPARNQVEAHYAWAWAQNIWSDMLG"
     gene            complement(3598..4044)
                     /gene="dut"
                     /locus_tag="AC16O9_01335"
     CDS             complement(3598..4044)
                     /gene="dut"
                     /locus_tag="AC16O9_01335"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR6515293.1"
                     /translation="MRRLEVRILDPRIREHLPHYATAGAAGLDLRACLEAPLELLPGA
                     SQLVRSGLAIHLGDPGLAAVVLPRSGLGAKHGIVLGNLVGLIDSDYQGEVMVSVWNRG
                     ANAFTIQPLERIAQLVVVPVVQVELAVVEEFAASARGAGGFGSTGR"
     gene            complement(4031..5242)
                     /gene="coaBC"
                     /locus_tag="AC16O9_01340"
     CDS             complement(4031..5242)
                     /gene="coaBC"
                     /locus_tag="AC16O9_01340"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR6515294.1"
                     /translation="MTLKGKRLLLGITGGVAAYKAAQLARQLARAGADVRVAMTEAAT
                     RFVTPVTLQALTAQTVWTDLWDPRVPDNMGHIELSRDRELIVVAPASADFLGKLAGGL
                     ADDLLSTLCLARRCPLLVAPAMNVEMWENPAMQRNLHTLRGDGVHFAGPAAGDQACGE
                     VGMGRMLEPDDLVAEIEAFFQPKLLAGKRVVVTAGPTEESIDPVRVITNTSSGKMGYA
                     VARAAREAGARVTLVSGPTALAAPVGVECVRVRSAEQMFEAVKKAVPGSDVFFSVAAV
                     SDYRAKQRAAQKIKKSGGAAFGLELIENPDILAWVAGQKDAPFCVGFAAESEKLAEHA
                     RAKRAKKAVPLLAANLAQHALGADDNDITLFDERGEHPLGRAPKLELARRLVAHVAGM
                     LAGQKRARAKA"
     gene            5304..5978
                     /gene="radC"
                     /locus_tag="AC16O9_01345"
     CDS             5304..5978
                     /gene="radC"
                     /locus_tag="AC16O9_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR6515295.1"
                     /translation="MGIADWPPQERPRERLLAQGAGRLTDAELVAVCLRSGIRGKSAV
                     DLARELIGRYQGLAGLLGAEAADLRRVRGLGAAKAAQLAAVLELARRTLREQLRSGDA
                     LTTPGAVRDYLRLALGGRTHEVFVCVHLDAQHRVIAAEELFRGTLTQTSVYPREVVKA
                     ALAANAAAVIFAHNHPSGVAQPSQADELLTRQLREALALVDVRVLDHFVVAGNQALSF
                     AERGLL"
     gene            6010..6267
                     /gene="rpmB"
                     /locus_tag="AC16O9_01350"
     CDS             6010..6267
                     /gene="rpmB"
                     /locus_tag="AC16O9_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR6515296.1"
                     /translation="MSRVCQVTGKKPMKGNLVSHANNKTKRVFQPNLHYRRFWLESQN
                     RWVRLRVSTAGLRKIDKLGVEAVLAEIRAKAKALNKQKEAR"
     gene            6267..6422
                     /gene="rpmG"
                     /locus_tag="AC16O9_01355"
     CDS             6267..6422
                     /gene="rpmG"
                     /locus_tag="AC16O9_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR6515297.1"
                     /translation="MRDKIKLESTAGTGHFYTTTKNKRTTPDKMEIKKYDPKARKHVL
                     YKETKLK"
     gene            complement(6497..7669)
                     /locus_tag="AC16O9_01360"
     CDS             complement(6497..7669)
                     /locus_tag="AC16O9_01360"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR6515298.1"
                     /translation="MIFSGLLDLPWWGYVLVALGLTHVTIASVTIFLHRHQAHRALDL
                     HPIASHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETADDPHSPQVLGINRVLWGGVFL
                     YVKESYNRETMDRYGHGTPDDWVERNIYSRFAILGLTLMGLVNVGLFGVVPGMLILAT
                     QIVWIPFWAAGVINGIGHYWGYRSFDIADASKNIVPWGILIGGEELHNNHHAYATSAK
                     LSAKWYEFDIGWMYIRVLEGLGLATVKKVAPTPRFEPAKTMCDYDTLHAVIANRYDVL
                     ARYATALKRTWSDEVERLQHWSPRDAEVLKPLKRALVRIGYGAETDRARVAEALKPSR
                     PLQVAVSMREELIALWERSNASKEQLLRQLQDWCHRAEASGIVPLMDFSRRLRSYA"
     gene            complement(7810..9060)
                     /locus_tag="AC16O9_01365"
     CDS             complement(7810..9060)
                     /locus_tag="AC16O9_01365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR6515299.1"
                     /translation="MIYRAQLARAEALLAELLAAAGPADAAVSRYFRAHRNLGQQDRA
                     FVAETVYAVLRRRRSLEAAAQSVAPRDLAIAALVRVRGLSGRALEDVVREEERALVAR
                     VRAAKSESFPAAVRADLPDWLWERLAALHGEDEAMRIAQGLLNPAPLDLRVNLAKLGR
                     DEALAQLRASGIEGQPTPFSPAGIRLVGKPAINRHPMFTQGAIEVQDEGSQVLAYLLA
                     PRRGEMIADYCAGAGGKTLALAMLMRGTGRIYAMDVSARRLAELAPRAARAGVTNVHP
                     LVLAGDGDARARRLAGKLDRVLVDAPCSGFGTLRRNPELKWRHGAAAITELAGKQAAI
                     LEAAARLLKPGGRLVYATCSILPEENEVIAEKFAASHADFAPVSCRELLAAQRIEIDC
                     EARLRLWPHRHGCDGFFAAAFERR"
     gene            complement(9057..9737)
                     /locus_tag="AC16O9_01370"
     CDS             complement(9057..9737)
                     /locus_tag="AC16O9_01370"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR6515300.1"
                     /translation="MRNWFLAIALSLAAAAQASPPARVEIHYDVLHNGGPLATVTHVL
                     EHDSDSYRLRETWEGSGLLSLLGEIRRTSHGRVTPDGLRPLEYVDERPRREPARARFS
                     WEAGTLTQEFRDGPQKQPLPAHAQDRLSFLFAPAFHAPGSGSLEYHVADGKGVAHYVF
                     EIAGRERVTVPAGTFDALHLVKRDDDGRSTQIWLDAQRSYLPLRVLVVQKDGTRVDQV
                     ATRFAPPR"
     gene            complement(9706..>10020)
                     /gene="purN"
                     /locus_tag="AC16O9_01375"
     CDS             complement(9706..>10020)
                     /gene="purN"
                     /locus_tag="AC16O9_01375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR6515301.1"
                     /translation="HPSLLPEFPGLDTHARALAAGAKRHGCTVHFVTAELDHGPIIAQ
                     ASVPVLPGDTAESLAARVLAQEHLIYPRALRWFLDGLLSLEDGKVYVKGNHAQLVSSD
                     RA"
BASE COUNT         1717 a         3437 c         3353 g         1513 t
ORIGIN      
        1 atctcgcacg gcatcaccaa gacgccgcag ccgcacgccg gcaaggccga gcaggaggcc
       61 ggcgacgagg cgcaggccga gccctccaac agcccgctcg acaactacac gcagaacctg
      121 aacgcgcttg cgctcgccgg caagatcgat ccgttgatcg gccgcgagcg cgagctggag
      181 cgcgtgatcc agacactgtg ccgccggcgc aagaacaatc ccctgctggt gggcgaggcc
      241 ggcgtcggca agaccgcgat cgccgagggg ctggcgcgca gaatcatcga gtcgaatgtg
      301 cccgagctgc tcgcgcgcag cacggtctac gcgctcgata tgggggccct cctcgccggc
      361 accaagtacc gcggcgactt cgagcagcgc ctgaaggcgg tgctgaagca gttggtggac
      421 aaccccaacg cgattctctt catcgacgag atccatacgg tcatcggcgc cggcgccgcc
      481 tcgggcggca cgctggacgc ctccaatttg ctgaagcccg tgctctccac cgggcagctg
      541 aagtgcatcg gcgccaccac gtacaacgag taccgcggcg tgttcgagaa ggaccacgcc
      601 ctgtcgcgcc gcttccagaa gatcgacgtc gtcgagccct cggtggacga gacggtggag
      661 atcctgaagg ggctgaagac gcgctttgag gcacaccaca gcgtcaagta cacggcgacg
      721 gcgctcagct cggcagccga gctctctgcg cgctacatca acgaccgcca ccttcccgac
      781 aaggcgatcg acgtcatcga cgaagcgggt gccgcgcagc gcatcgcgcc caagagccgg
      841 caaaagcgcg tcatcggcaa gggcgagatc gaggagatcg tggcgaagat cgcgcgcatc
      901 ccgccgcgca gcgtctccag cgacgaccgc ggcgcgctcg ccaagctcga ccgcgacctg
      961 aaggcagtgg tgttcggcca ggaccaggcc atcgacgccc tcgcctcggc catcaagatg
     1021 gcgcgctcgg gactcggcaa tccgcagaag cccatcggca gcttcctgtt ctccgggccg
     1081 acgggggtcg gcaagaccga ggtcgcgcgc cagctcgcct actgcatggg catcgagctg
     1141 atccgcttcg acatgtcgga gtacatggag cgccacgcgg taagccgcct gatcggcgcg
     1201 ccgccgggct acgtcggctt cgagcagggg ggcctgctga ccgaggcgat caccaagaag
     1261 ccctatgcgg tgctgctgct cgacgagatc gagaaggcgc acccggacat cttcaacatc
     1321 ctgctgcagg tgatggacca cggcacgctc accgacaaca acggccgcaa ggccgacttc
     1381 cgcaacgtgg tgatcgtgat gaccaccaac gcgggcgccg agtcgctgac caagaacgcc
     1441 atgggcttca cgccctcggt gaaggcaggc gacgagatgg aagccatcaa gaagatgttt
     1501 acgcccgaat tcaggaaccg cctggacgcg gtgatctcgt tccgcgcgct ggatcacgac
     1561 gtcatcatgc gcgtggtgga caagttcctc atccagctcg aggagcagct gcaggagaag
     1621 aaggtcgagc cgaccttcac gccgaagctg aaggagcacc tggccaagaa gggtttcgat
     1681 cccttgatgg gtgctcgccc gatggcgcgc ctcatccagg acaccatccg ccgcgcgctc
     1741 gccgacgagc tgctgtttgg caagctggcc catggcggga aggtcaccgt ggacatcgac
     1801 gacgccggca acgtggcgct gcagttcgag gccgaggcgc cggcgccgcc gccggcgaaa
     1861 gtgccggtca cctgagcccg gatcccggcg cagccgcgcc gggttgccag cgtccggggc
     1921 tgctccgcgt caattgcgcg cgcagaccat cttgacacat gccgcttgct cagacgagac
     1981 tccccggcac agcgactgaa tctccgggga ggactccgca tgcgaattgc tcttgtttgc
     2041 gcggctgcgc tggcggccgc gccccttgtc gtgagcgcgc aggacgcggg cagccgcctg
     2101 tctgcatcgt gcgccatgtg ccatggcact gccggccaca gcgtcggcgg caacgcgcct
     2161 ctggccggca tgccaagaga cgatctagtg cgtgccatga aggaattcca gtctggcgcg
     2221 cggcccgcaa ccgcgatgca tcaaattgcc aagggctaca gcgaccgtga aatcgagcag
     2281 atcgccgcgt acttcgcggc gcagaagaag tagggggacg ccatggccaa gacattcgat
     2341 cgacgcaact tcctcaagct gagcggcgcc gcggcggcgc tcgccgccac gggctgcgcc
     2401 accagcggcg gcgccaaggc gcgcgtggtg gtgatcggcg ggggcttcgg cggcgccacc
     2461 gcggccaagt acatacgcat gtggggcccg gccatcgagg tcgtgatggt ggaacgcaac
     2521 actgacttcg tctcctgccc gatctccaac ctggtctttg gcggctcgaa gaccatggcc
     2581 gacatcacct acggctacag cgggctttcg aagtacggcg tgcaggtcgt gcgcgacgaa
     2641 gtcacggcgg tggacacggg cgccaagtca gtgcgcctcg cgcgcggcgg tgagctcaag
     2701 tacgaccggc tggtggtgtc tcccggtatc gacttcatgt acgagcaaat cgccggctat
     2761 gaggcggcaa tggactcaag ccgcgtgctg cacgcctgga aggcgggtcc gcagaccgtc
     2821 gccctgcaaa ggcagctcgc cgagatgcgc ctgggcggcg tctacgtcct cgcggtccct
     2881 ctggcaccct accgctgccc gccggggccc tacgagcgcg cgagccaggt ggcgcagtac
     2941 ttcaagaagc acaatccgcg cgccaaggtc ctgatcgtcg acgccaaccc ggacgtgacc
     3001 tccaagggca agctgttcaa gcaggcttgg gccgacctct atccgggcat gatcgagttc
     3061 cgcggcaatt cgaaggccat cggcgtcgac ccgggaaccg gcaccgtcaa gaccgaggtc
     3121 gaggaagtca agggcgatgt cctgaacgtg gtgccggcgc aacgtgccgg aaccatcgcc
     3181 gccaaggcgg gcctgatcac ggccaacgac cgctggtgcg gggtcgactg gcgcacgacc
     3241 gagtcgaccg tggccaaggg cgtccacgtc cttggcgacg cgacgctgtc cgcgcccggc
     3301 atgcccaagt ccggcagcat ggccaaccag caggccaagg tctgcgccgc tgccgtggtg
     3361 gccatgatca acggcagcga acccaacccg aacccgaaga tcgccaacat gtgctacagc
     3421 ttcgtttccg acgatgaagt gatccacgtg acctcggtgc acgagctgga tgccggcaag
     3481 aagaccttcg tgccgatcaa gggagcgggc ggattgtcgc cggcacgcaa tcaggtcgaa
     3541 gcgcactacg cctgggcctg ggcgcagaac atctggtccg acatgttggg ctgagggcta
     3601 gcgcccggta ctgccgaagc cgccggcgcc gcgcgcgctg gcggcgaatt cctccaccac
     3661 cgccaactcg acctgcacca ccggcacgac caccagctgc gcgatgcgct cgagcggctg
     3721 gatggtgaac gcgttcgcgc cccggttcca caccgagacc atcacttcgc cctggtaatc
     3781 cgaatcaatc aggccgacga gattgccgag cacgatgccg tgctttgcgc ccaggcccga
     3841 gcgcggcagc acgacggccg cgagacccgg atcgccgagg tggatggcga gccccgagcg
     3901 caccagctgg ctggcgcccg gcagcaactc gagcggtgcc tcgaggcagg cgcgcaggtc
     3961 taggccggcg gcgcccgcgg tggcgtagtg cggcaggtgc tcgcggatcc tcgggtcgag
     4021 gatccggacc tcaagccttc gcacgcgcgc gcttctgtcc cgccagcatg ccggcgacgt
     4081 gcgcgaccag ccggcgcgcg agctcgagct tcggcgcgcg cccgagcggg tgctcgccgc
     4141 gctcgtcgaa cagcgtgatg tcgttgtcgt cggcgccaag cgcatgctgc gccaagttgg
     4201 ccgcgagcag cggcaccgcc ttcttcgcgc gcttggcgcg cgcgtgctcg gccagcttct
     4261 cgctctcggc ggcgaagccg acgcagaacg gcgcgtcctt ctgccccgcc acccaggcga
     4321 ggatgtccgg gttctcgatc agctcgagac cgaaagcggc gccgccgctc ttcttgatct
     4381 tctgcgccgc gcgctgtttc gcgcggtagt cggataccgc cgccaccgag aagaacacgt
     4441 cgctgccggg caccgccttc ttgactgcct cgaacatctg ctccgcgctg cgtacccgca
     4501 cgcactcgac gcccaccggc gctgcaagcg ccgtcgggcc gctcaccagg gtcacgcgcg
     4561 cacccgcttc gcgcgcggcg cgcgcgacgg cgtagcccat cttgccggag ctcgtgttgg
     4621 taatcacgcg cacgggatcg atcgactcct cggtagggcc cgcggtgacc acgacgcgct
     4681 tgcccgcgag cagcttcggc tggaagaagg cctcgatctc agcgaccaag tcatcgggct
     4741 cgagcatgcg gcccatgccc acttcaccgc aggcctgatc gccggcggcg gggcccgcaa
     4801 aatgcactcc gtcaccgcga agcgtgtgca ggttcctctg catcgctggg ttctcccaca
     4861 tctccacgtt catcgccggc gcgacaagca gggggcagcg gcgcgcaagg cacagggtgg
     4921 agagcaggtc gtcggcaagg cccccggcca gcttgccaag aaaatcggcg ctcgccggcg
     4981 ccaccacgat cagctcgcgg tcgcgcgaca actcgatgtg ccccatgttg tcggggacgc
     5041 gcgggtccca cagatcggtc cacacggtct gcgccgtgag cgcttgcaga gtcacaggcg
     5101 tgacgaagcg ggtggcggcc tcggtcatcg ccacgcgcac gtcggcacct gccctggcga
     5161 gctggcgtgc gagctgcgcg gccttgtagg cggccacgcc gccggtgatg cccagcaaca
     5221 gccgcttgcc tttcaaggtc atcgtgcgtg gggctggcgc gttaatggga aggacattct
     5281 aggggaattc gggaggcatc gccttgggca tcgccgactg gccaccgcag gaacggccgc
     5341 gcgagcggct gctggcgcag ggtgcaggaa ggctgaccga cgcggagctg gttgccgtgt
     5401 gcctgcgctc gggcatccgc ggcaagagcg ccgtggacct ggcgcgcgag cttatcgggc
     5461 gctaccaggg cctcgccggc ctgctcggcg ccgaagccgc cgacctgcgc cgagtcaggg
     5521 ggcttggtgc cgccaaggcg gcgcaactcg ccgcggtgct cgagctcgcg cggcgcaccc
     5581 tgcgcgagca gctgcggtcc ggcgacgcgc tcaccacgcc cggagcagta cgcgactacc
     5641 tgcgcctcgc gctcggcggg cgaacgcacg aagttttcgt ctgcgtccac cttgacgcgc
     5701 agcaccgcgt catcgccgcc gaggagctgt tccgaggcac gctcacgcaa accagcgtct
     5761 acccacgcga ggtggtcaag gccgctctgg cggccaatgc agccgccgtg atctttgctc
     5821 acaatcaccc ttccggcgtg gcgcagcctt cgcaggccga cgagctgctc acgcgccagc
     5881 tgcgcgaggc gctcgcgctg gtggacgtgc gcgtgctcga ccatttcgtc gtcgccggca
     5941 accaggcact ctccttcgcc gagcggggcc tgctataatc cgcgcctttc gcgacccccg
     6001 gagcctgcaa tgtcccgcgt ctgccaagtc accggcaaga agccgatgaa aggaaacctg
     6061 gtttcgcacg ccaacaacaa gaccaagcgt gtgttccagc ccaacctgca ttaccgccgc
     6121 ttctggttgg aaagccagaa tcgctgggtg cgtttgcggg tgtccacggc cggcctgcgc
     6181 aagattgaca agctgggggt cgaggccgtg ctggccgaaa tccgcgccaa ggccaaggcg
     6241 ctcaacaagc agaaggaggc ccgctgatgc gcgacaagat caagctcgag tcaacggccg
     6301 gcaccggcca tttctacacc accaccaaga acaagcgcac tacgcccgac aagatggaga
     6361 tcaagaagta cgatcccaag gcacgcaagc acgtgctcta caaggaaacc aagctgaagt
     6421 aggcgcggcc ttctgccgca catgtgaaaa ggcccgcgcg agcgggcctt ttttattacc
     6481 gcgttccggc tccgcgttag gcgtagctgc gcaggcgccg cgagaagtcc atcagcggca
     6541 cgatgccgct cgcctcggcg cggtggcacc agtcctggag ctggcgcagg agctgctcct
     6601 tggaggcgtt ggagcgctcc cacagggcga tcagctcttc gcgcatgctc accgcgacct
     6661 gcagcggcct ggagggcttg agtgcttcgg caacgcgcgc gcggtcggtt tcggcgccgt
     6721 agccgatgcg caccagagcg cgcttcagcg gcttgagcac ctcggcgtcg cgcggcgacc
     6781 agtgctgcag gcgctcaacc tcgtcactcc aggtgcgctt cagcgcagtg gcgtagcgcg
     6841 ccagcacgtc gtatcggttg gcgatcacag catgcagggt gtcgtagtcg cacatggtct
     6901 tcgccggctc gaagcgcggc gtgggcgcca cctttttcac cgtggcgagt ccgagcccct
     6961 caaggacgcg gatgtacatc cagccgatgt cgaactcgta ccacttggcc gaaagcttcg
     7021 ccgaggtcgc gtaggcgtgg tggttgttgt gcagctcttc gccgccgatc aggatgcccc
     7081 agggcacgat gttcttgctg gcgtcggcaa tgtcgaagct gcgatagccc cagtagtgac
     7141 cgatgccgtt gatgacgccc gccgcccaga acgggatcca gacgatctgc gtcgcaagga
     7201 tcagcatgcc cggcacgacg ccgaacaagc caacgttcac caggcccatc agcgtcaggc
     7261 cgaggatggc aaaacgcgaa tagatgttgc gctctaccca gtcgtccggc gtgccatggc
     7321 cgtagcgatc catggtctcg cggttgtagg actccttcac atagaggaac acgccgcccc
     7381 acagcacgcg gttgatgccc agcacctggg ggctgtgcgg gtcatcggca gtctcgcact
     7441 tggcgtggtg cttgcggtgg atcgccgccc actccttggt gaccatgccg gtcgtcagcc
     7501 acagccagaa acggaagaag tgcgaggcga tcgggtgcag gtcgagcgcc cggtgcgcct
     7561 gatggcggtg caggaagatg gtcaccgagg caatggtgac gtgtgtcagc ccgagcgcta
     7621 cgagcacgta gccccaccag ggcagatcga gcaatccaga gaaaatcatc agtagtttct
     7681 agtagttata taaggttagg ggcacgattc tacgcgattg aaccacccgc gcaaccgggg
     7741 cttttccctg ctcgcgggca atatttcacc gtcggctcta agcgccggcc gcgcaaaggg
     7801 aatcggccgt cagcggcgct cgaaagcggc ggcgaaaaag ccgtcgcagc catgccggtg
     7861 cggccacaag cgcagccgcg cttcgcaatc gatctcgatg cgctgcgcgg ccagcagttc
     7921 tctgcaggac acgggcgcga agtcggcgtg cgacgcggcg aacttttcgg cgatcacctc
     7981 gttttcttcc ggcaggatac tgcaggtcgc gtacaccagg cggccgcccg gcttcagcaa
     8041 ccgcgctgcc gcctccagga tcgccgcctg cttgcccgcc agctcggtga tcgccgcggc
     8101 gccatggcgc catttcagct ctgggttgcg gcgcagtgtt ccgaagccac tacaaggggc
     8161 gtccacgagc acccggtcga gcttgccggc gagacgcctg gcacgcgcat cgccgtcgcc
     8221 cgccagcacc agcgggtgca cgtttgttac gccggcacgc gctgcgcgtg gcgcaagctc
     8281 ggccaggcgg cgcgccgaga cgtccatggc gtagatgcgc ccggtgccgc gcatcagcat
     8341 ggcgagcgcg agcgtcttgc cgccggcgcc ggcgcagtag tcggcgatca tctcgccgcg
     8401 tcggggcgcg agcaggtacg cgaggacctg gctgccttcg tcctgcacct cgatcgcacc
     8461 ctgcgtaaac atcgggtggc gattgatcgc cggcttgcct accaggcgaa ttcccgccgg
     8521 ggagaagggc gtcggctgtc cctcgatgcc gctcgcacgc agttgcgcga gcgcctcgtc
     8581 ccgtcccagc ttggcgaggt tgacgcgcag gtcgagcggt gccgggttga gtagaccctg
     8641 cgcaatgcgc attgcctcgt cctcgccgtg cagcgccgcc agccgctccc aaagccaatc
     8701 gggcagatct gcgcgcacgg cggcgggaaa agactcgctc ttcgcggcgc gtacgcgcgc
     8761 caccagcgcc cgctcctcct cgcgcaccac gtcctctagg gcacgtcccg acaggccgcg
     8821 cacccgcacc agcgcggcga tcgccaggtc gcgtggcgcc actgattgcg ccgcggcctc
     8881 gagcgagcgg cggcggcgca gcaccgcgta taccgtctcg gcgacgaagg cgcgatcctg
     8941 ctggccgaga ttgcggtgcg cgcgaaagta cctcgacacc gcggcgtcag cggggccggc
     9001 ggcggctaag agctcggcga gcagcgcctc ggcccgggcg agctgggcgc gatagatcat
     9061 cgtggcggag cgaagcgcgt cgcgacctgg tcgacgcgcg tgccgtcctt ttgcaccacc
     9121 agcacgcgca gcggcagata tgagcgttgc gcgtcgagcc agatctgcgt gctgcggccg
     9181 tcgtcgtcgc gctttaccaa gtgcagcgca tcgaacgtgc ccgctggcac cgtcacgcgc
     9241 tcgcgtcccg cgatttcgaa cacgtagtgc gccaccccct ttccgtccgc gacgtggtat
     9301 tcgagcgaac cggatcccgg cgcgtggaag gcgggtgcaa acagaaagct caaccggtcc
     9361 tgcgcgtgcg ctggcagcgg ttgcttctgc gggccgtcgc ggaactcctg cgtcagggta
     9421 cccgcctccc aggaaaagcg cgcgcgcgcc ggctcgcggc gcggccgctc gtccacgtat
     9481 tcgagcggtc gcaggccgtc cggcgtcacc cggccgtggc tcgtgcgccg gatttcgccg
     9541 agcagtgaca gcaacccgct gccttcccag gtttcgcgca ggcggtagct gtcgctgtca
     9601 tgttccagca catgcgtcac ggtggcgagc gggccgccgt tgtgcagcac gtcgtagtgg
     9661 atctcgacgc gcgcgggcgg ggacgcctgc gccgcagccg cgaggctaag cgcgatcgct
     9721 agaaaccagt tgcgcatggt tgcctttgac gtacaccttg ccatcctcga ggctcagcag
     9781 accatcgaga aaccagcgca acgcgcgcgg atagatcagg tgttcctgcg cgagcacccg
     9841 cgccgcgagg gactccgccg tgtcgcccgg cagcacgggc accgacgcct gggcgatgat
     9901 cggcccatgg tcgagctcgg cggtcacgaa atgcacggtg cagccgtgcc gcttcgcgcc
     9961 ggcagccagc gcgcgcgcgt gcgtatcgag acccgggaac tcgggcagca gggaggggtg
//
LOCUS       DBNHKE010000024         6225 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_56269, whole genome shotgun sequence.
ACCESSION   DBNHKE010000024 DBNHKE010000000
VERSION     DBNHKE010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_56269"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="AC16O9_01380"
     CDS             complement(<1..630)
                     /locus_tag="AC16O9_01380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6515302.1"
                     /translation="MTPKLPCPRPARVVLEGRYARLEPLALVHAEGLMAASADPASFE
                     YLFDVPPRDLEDLKSYIRLKAASEDPLFHSVIDKATGVAGGRQTFMRITPEHGVIEIG
                     NILWGPSIARTRVATEAFYLAAKYVFEDLGYRRFEWKCNDLNVPSKRAARRFGFAFEG
                     VFRQHMWTKGANRDTAWFAMLDRDWPRLRAAYERWLAPSNFDAAGRQREK"
     gene            complement(627..1457)
                     /locus_tag="AC16O9_01385"
     CDS             complement(627..1457)
                     /locus_tag="AC16O9_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6515303.1"
                     /translation="MRIALCNEVIAPMPFPQQCEYAAKLGYDGLEIAPYTLSEEPQRL
                     GSAQLAAARAAAEDAGVAITGLHWLLIKPAGLSISTRDDAVRKKTLDVMFALVEQCAE
                     LGGRYLVHGSPHQRRIDPGETRAAALARAQECFAAVAERAEKAGVMYCIEPLGSDQTP
                     LINTLKEAALMVEAIGSRALRSMLDCSAAGRMESAPLAALVDRWLPNGMIAHVQVNDR
                     NRRGPGQGEQRFAPLFAALKRHGYAGDIAVEPFDYVPDGPGAAARAIGYVRGILEALP
                     "
     gene            1738..2523
                     /locus_tag="AC16O9_01390"
     CDS             1738..2523
                     /locus_tag="AC16O9_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515304.1"
                     /translation="MAQVAAQALLRVEGVGVRFGGIVALDSVSFDVEPGRIVGLIGPN
                     GAGKTTLFNCLSRLYECGSGDILFNGASLLRTPKHGIAALGIGRTFQNLALFRTMTVL
                     DNVMIGHHCRTRSGFLVNALRLPRVANEEHATLEKARNLIRFLGLDAVAGARVADLPF
                     GTAKRVELARALASDPKLLLLDEPASGLNHEEVGELGELIREIRDRLGLTVLLVEHHM
                     SLVMSISDRVVALNFGRKIAEGTPAEVQAHPEVIQAYLGGATH"
     gene            2523..3236
                     /locus_tag="AC16O9_01395"
     CDS             2523..3236
                     /locus_tag="AC16O9_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515305.1"
                     /translation="MAALLEVSGLRAAYGQTRVLHGIDFAMEEGTITTLLGANGAGKT
                     TTLRALCGMVKTEGEVRFTGERIDGKATEDVVRRGIAHVPDGRGTFTGLTTEENLRLG
                     AYTRKDRAQVESDLERVYGYFPRLMERREQQAGTLSGGEQQMLAVSRALMLRPRLMLL
                     DEPSFGLAPLIVQELFEILRTVNQKERVSMLVVEQNAAMALELADHAYLIETGRVVLS
                     GTAAEIRQNDAVRRSYLGY"
     gene            3244..4113
                     /locus_tag="AC16O9_01400"
     CDS             3244..4113
                     /locus_tag="AC16O9_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6515306.1"
                     /translation="MEEFLHQVLAGLATGGIYASLALALVMIYQATHLVNFAQGEMAM
                     FATYLAWSLINAGMPYWPAFLLTVVLAFVLGVAIERVIIRPVENAPVLAVVVVFIALL
                     VILNSVAGWIYTYTIKSFPSPFPAEPIFGQYITSHQGGAIGVTLVVLLLLFLFFRYTP
                     LGLAMRAAAQNPDSARLVGIRVGWMLALGWGLAAAVGGVAGMMVAPIVFLDPNMMGGV
                     LLYAFAAALVGGIDNPAGAVFGGFLVGVLENLLGAFVIGNELKLAVALVIIIGVLVVK
                     PSGFFGKVLVSRV"
     gene            4110..5081
                     /locus_tag="AC16O9_01405"
     CDS             4110..5081
                     /locus_tag="AC16O9_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6515307.1"
                     /translation="MSRRQVILIGLVLLAACVLPFVVSNYRTFQFTLALVYAIALLGL
                     NMLTGYNGQISLGHGAFYAVGAYTAAILMEHFGMPYWATIPIAAAVCLVAGFLFGLPA
                     LRLEGLYLALATFALAVSMPALLKYHHLEQWTGGVQGIVLLKPDAPFGLPLNPDQWLY
                     FFTLAITVVMFVLAWNILRGRMGRAMIAIRDQHIAAEAMGIHTAMVKSMTFGISAMYT
                     GIAGALGAIAIQFVAPDSFNIFVSIVFLVGIVIGGLASISGAIYGALFIQFVPNIADA
                     ISKAAPWAIFGIFLLGFVYLMPMGVAGAVRMAVARLTRKAQNPKPPQ"
     gene            5095..>6225
                     /locus_tag="AC16O9_01410"
     CDS             5095..>6225
                     /locus_tag="AC16O9_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6515308.1"
                     /translation="MKLRYVSIAALLAAALASPTAFAQKTIKIGNTNPYSGPASAYGT
                     IGKTIGAYWKMVNDKGGINGRQIEWISYDDGYSPPKTVEMARRLVEQDQVLLIFQSLG
                     TPPNSAIHKYMNAKKVPHLFVATGATKWNDPKNFPWTMGWQPNYQTEGRIYAEHILKT
                     NPKAKIAVLYQNDDYGKDYLKGFRDGLGAKASSMIVKEVSYEVADPTIDSQMVQLQAS
                     GADTFFNVTTPKFSAQAIRKAYDSGWKPTHYLNNVGASVGAALKPAGLDKSVGLITTV
                     YYMDPTDAQWANHPAVQEWKAFMAKYYPEGNLADGSNMYGYAVAQTMEQVLKQAGNDL
                     SRANIMKQAASLKDFRPTTLLPGIRINTSPTDFAPIESEQLAR"
BASE COUNT          998 a         2124 c         2093 g         1010 t
ORIGIN      
        1 cttctcacgt tggcggccgg cggcgtcgaa attcgaaggc gccagccagc gctcgtaggc
       61 ggcgcgcagc cgcggccagt cgcggtcgag catcgcgaac caggcggtgt cgcggtttgc
      121 gcccttcgtc cacatgtgct ggcggaacac gccctcgaac gcaaagccga agcggcgcgc
      181 agcgcgcttg gagggcacgt tgaggtcgtt gcacttccat tcgaagcgcc gatagcccag
      241 gtcctcgaac acgtacttcg ccgcgaggta gaacgcctcc gtcgcgacgc gcgtgcgcgc
      301 gatcgacggg ccccacagga tgttgccgat ctcgatcacg ccgtgctccg gggtgatgcg
      361 catgaaggtc tggcgaccgc cggcgacgcc ggtggccttg tcgatcaccg agtggaacag
      421 cggatcctcg gaggccgcct tcagccggat gtaggacttc aggtcctcga ggtcccgcgg
      481 cggcacgtcg aacagatatt cgaagctggc cgggtccgcc gaagccgcca tcaacccttc
      541 tgcgtgcacg agcgcgagcg gctcgagccg ggcgtagcgc ccctcgagca ccacgcgcgc
      601 tgggcgcggg cagggcagct tcggcgtcac ggcagcgctt cgaggatacc gcgcacgtag
      661 ccgatggcgc gcgccgcagc gccgggcccg tcgggcacgt aatcgaaggg ctcgaccgcg
      721 atgtcgccgg cgtagccgtg gcgcttgagc gcggcaaaca gcggcgcgaa gcgctgctcg
      781 ccctgcccgg ggccgcggcg gttgcggtcg ttcacctgca cgtgcgcgat catgccgttc
      841 ggcagccagc ggtccaccag cgcggcgagc ggcgccgatt ccatgcgccc tgcggcgctg
      901 cagtcgagca tgctgcgcag ggcgcggctg ccgatcgcct ccaccatcag cgcggcttcc
      961 ttcagcgtgt tgatgagcgg cgtctggtcg gagccgagcg gctcgatgca gtacatgacg
     1021 cccgccttct ccgcgcgctc agccaccgcc gcgaagcact cctgcgcgcg cgcgagcgcg
     1081 gcagcgcgcg tctcgcccgg gtcgatgcgc cgctggtgcg gcgagccatg cacgaggtag
     1141 cgcccgccca gctccgcgca ctgctccacg agcgcgaaca tcacatccag ggtcttcttg
     1201 cgcacggcgt cgtcgcgcgt ggagatggac aggccggccg gcttgatcag cagccagtgc
     1261 agtcccgtga tggcgacgcc ggcgtcctcg gcggccgcgc gcgccgcggc gagctgcgcg
     1321 ctgcccaggc gctgcggctc ctccgacagc gtgtagggcg cgatctccag gccgtcgtag
     1381 ccgagcttgg ccgcgtactc gcactgctgc gggaacggca tcggcgcgat cacctcgttg
     1441 cacagtgcga tgcgcacggc gctacctgcg cagatagccc ggcaccaggt ccaccatttc
     1501 gcgcaggcgc gtgcggtccg ggtcgcggct gctgccgttg ccgcgcctgc gcggcttcct
     1561 cgcgttcgcc atgtcaggcc gcgattatgc cccgccccgg cgtgacccgg acctcgacac
     1621 gcggcccggg catctcgttt cttgacggat gccaagttct cggccaacaa tccgccggaa
     1681 gaagggaaag cgcccgtcaa ctcgctgcga ggcgagatcc acaacgagga gggagcaatc
     1741 gcacaggttg ctgcacaggc attgttgcgc gtggagggcg tgggggtgcg cttcggcggc
     1801 atcgtcgcgc tcgacagcgt cagcttcgat gtcgagccgg gccgcatcgt cggcctgatc
     1861 ggcccgaacg gcgcgggcaa gacgacgctg ttcaattgcc tgtcgcgcct gtacgagtgc
     1921 ggcagcggcg acatcctgtt caacggcgcc tcgctcctgc ggaccccgaa acacggcatc
     1981 gccgcgctcg gcatcggccg caccttccag aacctcgcgc tgttccggac catgacggtg
     2041 ctggacaacg tcatgatcgg gcaccactgc cgtacccgga gcggcttctt ggtcaacgcg
     2101 ctgcgcctgc cccgggtcgc gaacgaggag cacgccacgc tggagaaggc gcggaacctg
     2161 atccgctttc tcggcctcga cgcggtcgcc ggcgcgcgcg tcgccgacct gccgttcggc
     2221 accgccaagc gcgtcgagct ggcgcgggcg ctggcgagcg acccgaagct gctgctgctc
     2281 gacgagccgg cgagcggcct gaaccacgag gaagtgggcg agctcggcga gctgatccgc
     2341 gagatccgcg accggctcgg ccttaccgtg ctgctggtcg agcaccacat gagcctggtg
     2401 atgagcatct cggaccgcgt ggtggcgctc aacttcgggc gcaagatcgc ggagggcacg
     2461 ccggccgagg tacaggcgca tcccgaagtg atccaggcct atctcggcgg agcgacgcac
     2521 tgatggcggc gttgctcgaa gtctccggcc tgcgcgccgc ctacgggcaa acgcgcgtgc
     2581 tgcacggcat cgatttcgcc atggaggaag gcacgatcac cacgctgctt ggcgccaacg
     2641 gcgccggcaa gacgaccacg ctgcgcgcgc tgtgcggcat ggtgaagacc gaaggcgagg
     2701 tgcgcttcac cggcgagcgc atcgacggca aggcgaccga ggacgtcgtg aggaggggca
     2761 tcgcccatgt ccccgacggc cgcggcacat tcaccggcct tacgaccgag gagaacctgc
     2821 gcctgggcgc ctatacgcgc aaggaccgcg cacaggtgga gtccgacctc gagcgcgtct
     2881 acggctactt cccccgcctc atggaacgcc gcgagcagca ggccggcacg ctctcgggcg
     2941 gcgagcagca gatgctcgcg gtgtctcgcg cgctgatgct gcgaccgcgc ctcatgctgc
     3001 tcgatgagcc ctcgttcggc ctcgcgccgc tcatcgtgca ggagctgttc gagatcctgc
     3061 gcacggtaaa ccagaaggag cgggtgagca tgctggtcgt ggagcagaac gcggcgatgg
     3121 cgctcgagct cgccgaccac gcgtacctca tcgagaccgg gcgcgtggtg ctgtcgggaa
     3181 ccgccgccga gatccggcag aacgacgccg tgcggcgctc gtacctgggc tactgaggac
     3241 gccatggaag aattcctgca ccaggtgctc gcgggactcg ccaccggcgg catctacgcc
     3301 agcctggcgc tcgcgctcgt catgatctac caggcgacgc acctcgtgaa cttcgcgcag
     3361 ggcgagatgg cgatgttcgc cacctacctt gcctggagcc tgatcaacgc cgggatgccc
     3421 tactggccgg cgttcctgct cacggtggtg ctcgccttcg tgctcggcgt ggcgatcgag
     3481 cgcgtgatca tccggccggt ggagaacgcc ccggtgctcg cggtggtcgt ggtgttcatc
     3541 gcgctgctcg tgatcctgaa cagcgtcgcc ggctggatct acacctacac catcaagagc
     3601 ttcccgagcc ccttccccgc cgagccgatc ttcggccagt acattacctc ccaccagggc
     3661 ggcgccatcg gcgtcaccct ggtggtgctc ctgctgctgt tcctgttctt ccgctacacg
     3721 ccgctcggcc tcgccatgcg cgctgcggcg cagaaccccg attcggcgcg cctggtgggg
     3781 attcgcgtgg gctggatgct tgcgctcggg tggggcctcg cggccgcggt ggggggcgtg
     3841 gccggcatga tggtggcgcc gatcgtgttt ctcgatccca acatgatggg cggcgtgctg
     3901 ctgtatgcgt tcgcagcggc gctggtgggc ggcatcgaca acccggcggg cgccgtgttc
     3961 ggcgggttcc tggtgggcgt gctcgagaac ctgctcggcg ccttcgtcat cggcaacgag
     4021 ctcaagctcg ccgtggcgct ggtgatcatc atcggcgtgc tggtggtgaa gccctcgggc
     4081 ttcttcggca aggtgctggt gagccgcgta tgagccgccg ccaggtcatc ctcatcgggc
     4141 tggtgctgct cgccgcgtgc gtgctgccct tcgtcgtgag caactaccgc accttccagt
     4201 tcacgctggc gctcgtgtac gcgatcgcac ttctgggcct caacatgctc accggctaca
     4261 acggccagat ctcgctcgga catggcgcgt tctacgcggt gggcgcctac acagcggcga
     4321 tcctcatgga gcacttcggg atgccctact gggccacgat cccgatcgcg gcggccgtat
     4381 gcctggtggc gggattcctg ttcgggctgc ccgcgctgcg cctcgagggg ctgtacctcg
     4441 cgctcgccac cttcgcgctc gccgtgtcga tgccggcgct gctcaagtac caccacctgg
     4501 agcagtggac cggcggggtg cagggcatcg tgctgctcaa gccggatgcg cccttcggcc
     4561 tgccgctgaa tcccgaccag tggctgtact tcttcaccct ggccatcacg gtcgtgatgt
     4621 tcgtactggc ctggaacatc ctgcgcggac gcatggggcg cgccatgatc gcgatccgcg
     4681 accagcacat cgccgccgag gcgatgggca ttcacaccgc catggtgaaa tcgatgacct
     4741 tcggcatctc ggccatgtac accgggatcg cgggcgcgct cggcgccatc gccatccagt
     4801 tcgtggcgcc ggacagcttc aacatcttcg tgtcgatcgt gttcctggtc ggcatcgtca
     4861 tcggcgggct cgcgtcgatc agcggggcga tctacggggc gctgttcatc cagttcgtgc
     4921 ccaacatcgc cgatgccatc tcgaaggccg ccccctgggc gatcttcggc atcttcctgc
     4981 tcggcttcgt ctacctgatg ccgatgggcg ttgcaggcgc cgtgcgcatg gccgtagcgc
     5041 ggctgacgcg gaaggcgcag aatccgaaac caccacagta aggaggaggc agccatgaag
     5101 ttgagatacg tatcgatcgc cgccctgctg gccgccgcgc tcgcgtcgcc gacggccttt
     5161 gcgcagaaga cgatcaagat cggcaatacc aatccgtaca gcgggcccgc gtcggcctac
     5221 ggcacgatcg gcaagacgat cggcgcctac tggaagatgg tgaacgacaa gggcggcatc
     5281 aacggccggc agatcgagtg gatctcctat gacgacggct acagcccgcc gaagaccgtg
     5341 gagatggcgc ggcgcctggt cgagcaggac caggtcctgc tcatctttca gtcgctcggc
     5401 accccgccga acagcgcgat ccacaagtac atgaatgcga agaaggtgcc gcacctgttc
     5461 gtcgcgaccg gtgcgaccaa gtggaatgac ccgaagaact tcccctggac catgggctgg
     5521 cagccgaact accagaccga gggccgcatc tacgccgagc acatcctgaa gaccaatccc
     5581 aaggcgaaga tcgccgtcct ctaccagaac gacgattacg gcaaggacta cctgaagggc
     5641 ttcagggacg ggctgggcgc caaggcctcg agcatgatcg tcaaggaagt ctcctacgag
     5701 gtggccgacc cgacgatcga ctcgcagatg gttcagctgc aggcctcggg ggcggacaca
     5761 ttcttcaacg tcaccactcc caagttctcc gcgcaggcca tccgcaaggc ctacgacagc
     5821 ggctggaagc caacgcacta tttgaacaac gtgggcgcat ccgtcggcgc cgcgctcaag
     5881 cccgcgggcc tagacaagtc ggtggggctg atcaccaccg tgtactacat ggatcccacc
     5941 gacgcgcagt gggcgaatca tcccgcggtg caggagtgga aggcgttcat ggcgaagtac
     6001 tacccggaag gcaacctggc cgacggctcc aacatgtacg gctacgcggt ggcgcagacc
     6061 atggagcagg tgctcaagca ggcgggcaac gacctgagcc gggccaacat catgaagcag
     6121 gccgcgagtc tcaaggattt ccggccgacg accctgctcc ctggcattcg catcaatacg
     6181 tcgccgacgg acttcgcacc gatcgaatcg gagcagctgg cacgc
//
LOCUS       DBNHKE010000025         8229 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_532563, whole genome shotgun sequence.
ACCESSION   DBNHKE010000025 DBNHKE010000000
VERSION     DBNHKE010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_532563"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="AC16O9_01415"
     CDS             complement(<1..897)
                     /locus_tag="AC16O9_01415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6515309.1"
                     /translation="MALKRPKGSAGTLVVLEHVSRLLKGNPLGDPHVRKLGVWLPPQY
                     DDWGKQRFPVLYDLVGFTGSGLAHTAWKSFGDNVPERAARLIHEKKMGPAIIVFPDCF
                     TALGGNQYVNSSAIGDYADYLTKEIIPFVDKEFRTLPSREHRGCFGKSSGGYGSIIHG
                     MKYARYWGAIANHSGDAYFDFVYWHDWPNTLNELAKHRAPKRKAGAYDARREARRKGL
                     EQGRDDGRIKRFLQHVWKKEKLAGKEVHCIMNLCMAATYDPDPRAPNGFRIPFNAETG
                     ELIAARWKNWQRHDPINLVSRYK"
     gene            complement(911..3832)
                     /locus_tag="AC16O9_01420"
     CDS             complement(911..3832)
                     /locus_tag="AC16O9_01420"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6515310.1"
                     /translation="MTAMIADQKERDRALDAACSFIVQAPAGSGKTDLLVKRYLKLLQ
                     EVAKPEEVVAITFTKKAAAEMRQRVLEAVPAELAPRLRIQTIDALCAALTARMPVLAK
                     FGAQPRISEDPALLYHEAAARTLAEVTPPVARLLAHLDNFVAGATGLLAAMLERRDQW
                     LRSTGRAPTRKELELALRVERGRLLQAARALHAQASPALAREMLRKDGKWRAGAPEDL
                     RAVPGLEEMLGMLLIMPPETFSDQQWEALEAILALLNPAVAQLRVLFAERGEVDFTEV
                     AHGALAALGSADEPTDLLLSMDSQVRHLLVDEFQDTSNSQWELLERLTGGWQEGDGRT
                     VFVVGDPMQSIYRFRDAQVGLFLRARREGLPNVRLERLTLSTNFRSQGAIVSWVNRVF
                     PQVLPAVEDEASGAVPYSASVAFHEADAEGEPSLELFADREGEAKRVVALLSEARGRT
                     AILVRNRSHLDSIVPALKSAGVRFRAVEIEQLGERQVVQDLFALTRALTHLGDRVAWL
                     AVLRAPWCGCTPEELAAYFEGRFETVWERIQDEKRFSRLVSVLRPALENRLRGTLRDC
                     VEGVWLALGGPACVEDATDLEDAEIFFDELERLEVAGELEDFGALSRSLSQLFALPDV
                     EAGPEAVEIMTIHKAKGLEFDAVIVPGLDRPPRAARRPLFAWRSLPGGQLLLAPIDET
                     GGEAEPLYRYVRLLDREAEDIEAGRLFYVAATRAESRLYLLGCVKISEDGVVRLPSKR
                     SLLGLAWPALGESVRLPAAVAAEEAGSKPLPAGELIRLPSDFEMPRAPMAMAWKASGE
                     GREEAGPPFDWAKEAVRVAGIVVHAWLQRIAEDELRGWDVKRVDALRDRFASDLQRRG
                     IAPADLERAAALVVLGLKNAISDERGRWVLGPQKAARNEYRLRSRERSFRIDRVFEDA
                     NGNMWVVDYKASAHEGTGVEAFLDVQKVRYAAQLDAYAEALGGARRGLYFPLQKGWRE
                     W"
     gene            3831..4529
                     /locus_tag="AC16O9_01425"
     CDS             3831..4529
                     /locus_tag="AC16O9_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6515311.1"
                     /translation="MALKMWDLAAAEDDRRFSPYCWRAKMALAHKGLQAETVPWRFTE
                     QEAIAFSGQDKVPVLRDGDKEVHDSWAIANYLEDQYPVKPLFGSAQARALTYVFKIWT
                     ESTIHPPVLRAVILDLFAALHEKDQAYFRESREKRFGKTLEEFGANPKQAIADLRGVL
                     LPVRQQLVQAPYVCGTDPGFADYVLFGAFQWARAVSPQRLLEPDDPVFAWRERMLDLY
                     GGLARNAKGYPVWS"
     gene            complement(4535..7159)
                     /locus_tag="AC16O9_01430"
     CDS             complement(4535..7159)
                     /locus_tag="AC16O9_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6515312.1"
                     /translation="MPPAPVAKQELLQRLAAGHAARITVVTPNVRLARELAREFDAGQ
                     AAKDLRVWETADILPFGAFVERLYEDALYSDLAERLPLLLTSAQARALWEATIRASRW
                     GEGLLATPQAAADAARAWALAHAWRIDGALASYPGNDDAKAFAEWAREVARRCDKDGS
                     TDEARLADVVAPLLPEAALRKPKLLVAYAFDVVTPQEQDFFDACAKSGIEVRACAPGK
                     REGEAHRRVFPAAREELDAAAQWARAKLEAGAGRIGVVVPELERRRREVVRVFARVMR
                     PDHNLPGAEPRALPFNVSLGAPLADYPLVNAALAILELATHEVTFDHASKLMRSPFIA
                     GASSEMAQRALLDVSLRRIAPAKLNLGKLVALVKGAPILRQRLEALFGTMRPANDSPH
                     DWARHFTDLLEAMGFPGRSLNSDEFQTRAKFNATLAELARLERVAPRMRQEDALDALR
                     RLCGDTLFQPESPDAPIQVLGVFESEGLEFDALWVSGLTDQAWPLHASPNPFIPVALQ
                     KKAGIPEATPEATLERAKKITNGWLEAAPEVIVSHAAREEDRDLIGSPLISGVPSFSG
                     TPEAIRVDRYRDVLFAERRVERVKDGKAPALQTKTPRGGTRILVDQAACPFRAFARHR
                     LGAEALEEPVEGLDARDRGSLLHALMKELWTELKGSEGLKGDVAGAIERAATKAVQEE
                     AVEEPLASLERMRLAKLARDWLRVEAERAPFEVVALEEKRKLRVAGLELNGRIDRIDR
                     LASGGHAVIDYKTGRTTPQEWLGERPEDPQVPLYALNAEDVSAAAFAKLKAGEMRYMG
                     FSRAKDAIPKVQAAKNWETLVAGWRKELEALGTGFAAGEARVDPKKMLQTCRYCDLQP
                     LCRVYERVSALDEGGGDE"
     gene            complement(7255..7500)
                     /locus_tag="AC16O9_01435"
     CDS             complement(7255..7500)
                     /locus_tag="AC16O9_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515313.1"
                     /translation="MEYVAIGVIGLLVGLLVGRFLAGNNFSVAGDVVFALVGAFVFAV
                     GLRVTGLAPEGGAGSGAVMAGMGALAALLLRRVLRTV"
     gene            7576..>8229
                     /locus_tag="AC16O9_01440"
     CDS             7576..>8229
                     /locus_tag="AC16O9_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR6515314.1"
                     /translation="MDPGDPLLLARAQFGLNIAFHILFPTITIALAWFLIGFRVKYAR
                     TRDPRWLATYRLWVKIFALSFAMGVVSGITMSFQFGTNWPGYMMKVGNVAGPLLGYEV
                     LTAFFLEATFLGVMLFGMERVSTRVHILSAVMVALGTTLSAFWILALNSWMQTPAGHV
                     MDGDALIAGDWLAVIFNPSFPYRFTHVMLASGLTASFLVAGLSAWRLLRPAADEGARK
                     "
BASE COUNT         1244 a         2992 c         2675 g         1318 t
ORIGIN      
        1 cttgtatctg gaaaccaggt tgatggggtc gtggcgctgc cagttcttcc agcgcgccgc
       61 gatcaattcg cccgtttccg cgttgaacgg gatgcgaaag ccgttgggcg cgcgcgggtc
      121 ggggtcgtag gtcgccgcca tgcaaaggtt catgatgcag tggacctcct tgccggcgag
      181 cttctccttc ttccacacgt gctgcaggaa gcgcttgatg cggccgtcgt ccctgccctg
      241 ctccaggccc ttgcgtctgg cttcgcggcg cgcgtcgtag gcgccggcct ttcgcttcgg
      301 cgcgcggtgc ttggcgagct cgttcagcgt gttcggccag tcgtgccagt agacgaagtc
      361 gaagtaggcg tcgcccgaat gattcgcgat cgcgccccag tatcttgcgt acttcatgcc
      421 gtggatgatc gagccgtagc cgcccgagga cttgccgaag cagccgcggt gctcgcgcga
      481 cggcagagtg cggaactcct tgtcgacgaa ggggatgatt tctttggtga ggtagtcggc
      541 atagtcgccg atggccgagg agttgacgta ctggttgccg ccgagcgcgg tgaagcagtc
      601 ggggaagacg atgatcgccg gccccatctt cttttcgtgg atcaggcgcg ccgcgcgctc
      661 cgggacgttg tcgccgaagg acttccaggc ggtgtgcgcg aggcccgagc cggtgaagcc
      721 gacgaggtcg tagaggacgg ggaagcgctg cttgccccag tcgtcgtact gcggcgggag
      781 ccacacgccg agctttcgta cgtgcggatc cccaagcgga tttcccttga ggagccgcga
      841 cacgtgctcc aggacaacga gggtgcctgc agagcccttg ggtcgtttca gtgccatggt
      901 tcatcctttc tcaccattcg cgccagccct tctgcagcgg aaaatatagc ccccgccgcg
      961 caccgccgag ggcttccgcg tacgcatcga gctgcgccgc gtagcgcacc ttctgcacat
     1021 cgagaaacgc ctccaccccc gtgccctcgt gcgcgctcgc cttgtagtcc accacccaca
     1081 tattcccgtt ggcgtcctcg aatacccgat cgatgcggaa gctgcgctcg cggctccgca
     1141 gccggtactc gttgcgcgcc gccttctgcg gcccgaggac ccatcgaccg cgctcgtccg
     1201 agatcgcgtt cttcagcccc agcaccacca gcgcggcggc gcgctccagg tcggccggcg
     1261 caatcccgcg cctttgcagg tcgctggcaa agcggtcgcg cagcgcgtcg acgcgtttta
     1321 catcccagcc gcgcagctca tcctcggcga tgcgctgcag ccacgcgtgc accacgatcc
     1381 cggccacgcg caccgcttcc ttcgcccagt cgaatggcgg ccctgcttcc tcccgccctt
     1441 cgccggacgc tttccacgcc atggccatag gcgccctagg catctcaaaa tccgacggca
     1501 gcctaatcaa ctcccccgcc ggcaacggct tgctccccgc ctcctccgcc gccaccgccg
     1561 caggcaacct caccgactcc cccaaagccg gccacgccag cccgagcagc gaccgcttcg
     1621 aaggcagccg caccaccccg tcctcagaaa tcttcacgca ccccagtaag tacaaccgcg
     1681 actcggcccg cgtcgcggcg acgtagaaca gccgccccgc ctcgatgtcc tcggcctccc
     1741 ggtcgagcag ccgcacatac ctatagagcg gctcggcctc cccgccggtc tcgtcgatcg
     1801 gcgccaataa caactgcccc ccgggcaagg accgccaggc gaacaagggc cgccgcgcgg
     1861 cgcgcggcgg acgatcgagg ccgggaacga tgacggcgtc gaactccagg cccttggcct
     1921 tgtggatggt catgatctcc accgcctcgg ggccggcctc gacgtcgggc aaggcgaaca
     1981 attgactgag gctgcgcgaa agcgcgccga agtcctcgag ctcgcccgcg acttccaggc
     2041 gctcgagctc gtcgaagaag atctcggcgt cctccaggtc ggtcgcgtcc tcgacgcacg
     2101 ccgggccgcc cagcgcgagc cagacgcctt ccacgcaatc gcgcagcgtg ccgcgcagcc
     2161 ggttctccag agccggacgc aggacggaga cgaggcggga aaaacgcttt tcgtcctgga
     2221 tgcgctccca cacggtctcg aagcggcctt cgaaataggc ggcgagctcc tccggcgtgc
     2281 agccgcacca cggcgcgcgc agcacggcga gccacgccac gcggtcgccc aggtgagtca
     2341 gggcccgcgt cagcgcaaag aggtcctgca ccacctgtct ttccccgagc tgctcgatct
     2401 ccaccgcgcg aaagcgcacg ccggcgctct tcagggcggg cacgatcgaa tcgaggtgcg
     2461 agcggttgcg caccaggatc gcggtgcgcc cgcgcgcctc actgagcagc gccaccaccc
     2521 gcttcgcctc cccttcccgg tcggcgaaga gctcgagcga cggctcgccc tcggcgtcgg
     2581 cctcgtgaaa ggcgaccgag gccgaatacg gcaccgcgcc ggaggcctcg tcctccaccg
     2641 ccggcagcac ctggggaaag acccggttca cccacgacac gatcgccccc tgggaacgga
     2701 agttcgtaga cagcgtgagc cgctcgagcc gcacgttcgg cagcccctcg cgccgcgcgc
     2761 gcaggaacaa ccccacctgc gcatcgcgga accgatagat cgactgcatc ggatcgccca
     2821 ccacgaacac cgtgcgcccg tcgccctcct gccagccgcc ggtgaggcgc tccaggagct
     2881 cccactgcga gttggaggtg tcctgaaact catccaccag cagatgccgc acctgagaat
     2941 ccatcgacag cagcagatcg gtgggctcat ccgcagagcc gagcgcggcg agcgcgccgt
     3001 gcgccacctc ggtgaagtcc acctctccgc gctcggcgaa caaaacccgc agctgcgcca
     3061 ccgcggggtt cagcagcgca aggatcgcct ccagcgcctc ccactgctga tccgagaacg
     3121 tctccggcgg catgatgagg agcatcccga gcatctcctc cagccccggc accgcgcgca
     3181 ggtcctcggg cgcccccgcg cgccacttgc cgtccttcct caacatctcg cgcgcgagcg
     3241 cgggcgaggc ttgcgcatgc agcgcccgcg cggcctgcag cagccgcccg cgctcgacgc
     3301 gcagcgcgag ctccaactct tttcgcgtgg gcgcgcgccc ggtcgagcgc agccactggt
     3361 cgcgccgctc gagcatcgcg gccagcagcc cggtcgcgcc ggcgacgaag ttgtccaggt
     3421 gcgcaaggag ccgcgccacc ggcggcgtca cctccgcgag cgtgcgcgcg gcggcctcgt
     3481 ggtacagcag cgccgggtcc tcgctgatgc gcggctgcgc gccgaacttc gccagcaccg
     3541 gcatgcgcgc ggtgagcgcc gcgcacagcg cgtcgatggt ctggatgcga agccgcggcg
     3601 cgagctcggc cggcactgcc tccagcaccc gctggcgcat ctccgcggcc gccttcttgg
     3661 taaaagtgat cgccacgacc tcctcgggct tcgccacctc ctggaggagc ttgagatagc
     3721 gcttgaccag caggtcggtc ttccccgagc ccgcgggcgc ctggacgatg aacgagcagg
     3781 cggcgtccag cgcccgatcg cgctctttct ggtcagctat catcgccgtc atggcgctca
     3841 agatgtggga ccttgccgcc gccgaggacg accggcggtt ctcgccgtac tgctggcgcg
     3901 cgaagatggc gctcgcgcac aagggcctgc aggccgagac cgtgccgtgg cgctttaccg
     3961 agcaggaggc gatcgccttc tccggtcagg acaaggtgcc ggtgctgcgg gacggcgaca
     4021 aggaagtcca cgactcctgg gcgatcgcca attacctgga ggaccagtac ccggtgaagc
     4081 cgctctttgg cagcgcgcag gcgcgcgcgc tcacctacgt gttcaagatc tggaccgagt
     4141 cgacgatcca tccgccggtg ctgcgcgccg tgatcctgga tctgttcgcc gcgctgcacg
     4201 agaaggacca ggcgtacttc cgcgagtcgc gcgagaagcg cttcggcaag acgctggagg
     4261 aattcggcgc caatccgaag caggcgatcg ccgacctgcg cggcgtgctc ctgcccgtgc
     4321 gccagcagct ggtgcaggcg ccctacgtct gcggcaccga tcctggattc gccgactacg
     4381 tcctctttgg cgcctttcag tgggcgcgcg cggtgtcgcc gcagcgcctg ctcgagcccg
     4441 acgacccggt gttcgcgtgg cgcgagcgca tgctcgacct ctacgggggc ctcgcgcgca
     4501 acgccaaggg ctacccggtc tggagctagc gcgctcattc gtccccgccc ccctcgtcca
     4561 gcgcgctgac gcgctcgtat acgcggcata gcggctgcag gtcgcaatag cggcaggtct
     4621 gcagcatctt cttcggatcg acgcgcgcct cgcccgcagc gaagcccgtg ccgagcgcct
     4681 cgagctcctt tcgccagccg gccaccagcg tctcccagtt cttcgccgcc tgcaccttgg
     4741 ggatcgcgtc cttcgcgcgc gaaaacccca tgtagcgcat ctcgcccgcc ttcagcttcg
     4801 cgaacgcggc cgccgacacg tcctccgcgt tcagcgcata gagcggcacc tgcggatcct
     4861 cgggccgctc gccgagccac tcctgcggcg tcgtgcgccc ggtcttgtag tcgatcaccg
     4921 cgtgcccgcc ggaggcgagc cggtctatgc ggtcgatgcg gccgttcagc tccaggcccg
     4981 cgacgcggag cttgcgcttt tcctccagcg ccaccacctc gaagggcgca cgctcggcct
     5041 ccacccggag ccagtcgcgc gcgagcttcg cgaggcgcat acgctcgagg ctcgccagcg
     5101 gctcctccac cgcttcctcc tgcaccgcct tcgttgccgc ccgctcgatc gcgccagcca
     5161 cgtctccctt cagcccctcg ctgcccttca gctcggtcca cagttccttc atcagggcat
     5221 ggagcaggga gccccggtct ctcgcgtcca aaccttcaac aggctcttcc agggcttccg
     5281 ctccgagccg gtgcctggcg aaggcccgga aaggacaggc ggcctggtcc accaatatcc
     5341 tcgtaccacc ccgtggcgtc ttcgtctgca aggccggtgc cttcccgtcc ttaactcgct
     5401 ctactcgtcg ctccgcgaaa agcacgtccc tataccgatc gacccttatg gcctccggcg
     5461 ttccggaaaa gctcggaacg ccggagatca atggagaacc gatcaggtct cgatcctctt
     5521 cgcgggccgc gtggctcacg atcacttcag gtgccgcttc cagccatccg ttggtgatct
     5581 tctttgctct ttccagcgtc gcttccggcg tcgcctcggg gatgcccgct ttcttctgca
     5641 gcgcgaccgg aatgaagggg ttcgggctgg cgtgcagcgg ccaggcctga tccgtcagcc
     5701 cgctcaccca cagcgcgtcg aactccagcc cctcggactc gaacacgccg agcacctgga
     5761 tcggcgcgtc gggcgactcg ggctggaaca aagtgtctcc gcacagtcgg cgcagcgcgt
     5821 cgagggcgtc ctcctggcgc atgcgcggcg cgacgcgctc gagcctggcg agctcggcga
     5881 gggttgcgtt gaacttggcg cgggtctgga attcgtcgga gttgaggctg cggccgggga
     5941 agcccatcgc ctcgagcagg tcggtgaaat ggcgcgccca gtcgtgcggg gagtcgttgg
     6001 ccggtcgcat ggtgccgaag agcgcttcca atctctgcct caatatcgga gcgcccttga
     6061 caagggcgac gagtttgccg aggttgagtt tcgccggggc gattcttcgc aaggagacgt
     6121 cgagtaaagc acgctgtgcc atctcggagg acgccccggc gatgaaaggg gagcgcatga
     6181 gcttcgaggc gtgatcgaac gtcacctcat gcgtggccag ctcgaggatc gcgagcgcgg
     6241 cgttgacgag cgggtagtcc gcgagcggcg cgcccaggga gacgttgaag ggcagcgcgc
     6301 gcggctcggc gccgggcagg ttgtggtccg gacgcatgac gcgcgcgaag acgcgcacca
     6361 cctcgcgccg gcgccgctcg agctcgggga cgacgacgcc gatccggccc gcgcccgcct
     6421 cgagcttggc gcgcgcccac tgcgcggcgg cgtcgagctc ttcgcgtgcc gcagggaaca
     6481 cccgccgatg ggcctcgccc tccctcttcc ccggcgcgca ggcgcgcacc tcgatgcccg
     6541 acttggcgca cgcgtcaaag aagtcctgct cctgcggggt caccacatcg aaggcgtagg
     6601 cgacgagcag cttgggcttg cgcagcgccg cttcgggcag gagcggtgcg acgacgtcgg
     6661 cgaggcgcgc ctcgtcggtg ctgccgtcct tgtcgcagcg gcgcgcgacc tcgcgcgccc
     6721 actcggcgaa agccttcgca tcgtcgttgc cgggatagga cgcgagcgcg ccgtcgatgc
     6781 gccaggcgtg cgcgagcgcc caggcgcgcg ccgcgtcggc ggccgcctgc ggcgtggcga
     6841 gcagcccctc gccccagcgg ctcgcgcgga tcgtcgcctc ccacagcgcg cgcgcctgcg
     6901 cgctcgtgag cagcagcggc agccgctccg cgaggtcgga gtacagcgcg tcctcgtaca
     6961 ggcgctcgac gaaggcgccg aacggcagga tgtccgccgt ctcccagacc cgcaggtcct
     7021 tggccgcctg tccggcgtcg aactcgcgcg cgagctcgcg cgcgaggcgc acgttcgggg
     7081 tgacgacggt gatgcgcgcg gcgtggcccg cggcgaggcg ttgcagcagt tcctgcttgg
     7141 cgaccggcgc gggcggcatg cgccgacagt ttaaatgggg acagacctca tttttctcat
     7201 ctgcgaatca ggtgcaattg cgaaaaatga ggtctgtccc tatttattgc aaggctaaac
     7261 cgtcctcagc acgcgccgca gcagcagcgc cgcgagcgcg cccatgcccg ccatcaccgc
     7321 gccgctgccg gcgccgcctt ccggcgcgag gccggtgacg cgcagcccga cggcgaacac
     7381 gaacgcgccg acgagcgcga acacgacgtc gcccgcgacg ctgaagttgt tgccggcgag
     7441 gaagcggccg acgagcagcc cgaccagcag gccgatgacg ccgattgcga cgtactccat
     7501 gggaactctc ccgcagggaa cacccagcat acgcgctatt gcgcgcgcaa accggcgggg
     7561 gcgaaaatag gctggatgga ccccggcgac cccctgctcc tcgcgcgggc ccagttcggg
     7621 ctgaatatcg cgttccacat cctgttcccg accatcacca tcgcgctggc gtggttcctg
     7681 atcggctttc gcgtgaagta cgcgcgcacg cgggacccgc gctggctcgc cacctaccgc
     7741 ctgtgggtga agatcttcgc cctcagcttc gccatgggcg tggtctcggg catcaccatg
     7801 agcttccagt tcggcaccaa ctggccgggc tacatgatga aggttggcaa cgtcgcgggg
     7861 ccgctgctcg gctacgaggt gctgacggcg ttctttctgg aagcgacatt tctcggcgtg
     7921 atgctgttcg gcatggagcg ggtgtccacg cgcgtgcaca tcctttcggc ggtgatggtg
     7981 gcgctgggga cgacgctgtc ggcgttctgg atcctcgcgc tcaattcctg gatgcagacg
     8041 cccgccgggc acgtgatgga cggcgacgcg ctcatcgccg gcgactggct ggcggtgatc
     8101 ttcaacccgt cgtttcccta tcgcttcacg cacgtgatgc tcgcctcggg gctcacggcg
     8161 tcgttcctcg tcgccgggct ctcggcgtgg cgcctgctca ggcccgccgc ggacgaaggc
     8221 gcgcggaag
//
LOCUS       DBNHKE010000026         3890 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_585701, whole genome shotgun sequence.
ACCESSION   DBNHKE010000026 DBNHKE010000000
VERSION     DBNHKE010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_585701"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1103)
                     /locus_tag="AC16O9_01445"
     CDS             complement(63..1103)
                     /locus_tag="AC16O9_01445"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR6515315.1"
                     /translation="MTTLLRACGLALAIFAMAGSGTALAQAGSASNSQAQQERGETQP
                     GNNAPVWREVRSGNQGYTSLPGREMGVLVQSAGETWRQIRNGPVTVYGGWVLVLVVLG
                     IAAFFTTRGALKLREKPTGRMLERFNSVERVTHWTVAITFVVLAVSGLIMLFGKHVLL
                     PVIGYTLFAWLTALAKNLHNFIGPLFILGLLMMIVLYIKDNFPKAVDLQWVAKAGGLF
                     TGAHVPSGRFNAGEKSWFWVGVVAMGIIVSVTGLILLFPNFEQTRGTMQDAWTWHAVA
                     SLVVMAASLGHIYLGTIGVEGAYQAMRSGQVDEAWAKEHHELWYREVKGGAKSAPGGA
                     VPAGAPHMKEKQ"
     gene            complement(1138..1395)
                     /locus_tag="AC16O9_01450"
     CDS             complement(1138..1395)
                     /locus_tag="AC16O9_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515316.1"
                     /translation="MNRAALGAAVIAAAFLAAGCGEAQQVTVYKQGKYQGKPDTQAWN
                     NAPPAAELRGSAWNKGDRSSWETAINQRAMGQNEDNRIYKQ"
     gene            complement(1392..2018)
                     /gene="fdh3B"
                     /locus_tag="AC16O9_01455"
     CDS             complement(1392..2018)
                     /gene="fdh3B"
                     /locus_tag="AC16O9_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HIR6515317.1"
                     /translation="MARMKFLCDAERCIECNGCVTACKNENEVPWGVNRRRVVTINDG
                     VVGAEKSISVACMHCSDAPCMAVCPVDCFYKTEDGVVLHDKDLCIGCGYCFYACPFGA
                     PQFPQAGAFGLRGKMDKCTFCAGGPEADRTEAEFKKYGRNRLAEGKLPACAEMCSTKA
                     LLGGDGDVIADIYRERVLRRGKGSEVWGWGTAYGPGKAGAQPPAGGKS"
     gene            complement(2029..>3890)
                     /locus_tag="AC16O9_01460"
     CDS             complement(2029..>3890)
                     /locus_tag="AC16O9_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR6515318.1"
                     /translation="TQHTNGNAIVRASAILMLALGNAGRGGGGANIYRGHDNVQGATD
                     IGPNPDSLPGYYGIAPGSFAHWARVWNIDLDWLKKQYADEKMMTKPGITVSRWYDAIV
                     EKKENMGQDSNLRAVLFWGHAPNSQSRGLDMLEAMKKLELMVIIDPYPSATAAMMAMA
                     KKEGCYLLPAATQFETSGSVTASNRSIQWREQVIEPLFESRTDHMIMYQLAGKLGFAD
                     QLLGKKDGKQNIKLVKGKGGLDEPSMEDTTLEFNSGTWTIGYTGQSPDRLKAHMRNMH
                     VFDVKTLKAKGGKDAVTGYDLTGDYFGLPWPCWGNDKLKHPGSPNLYNIEKHVMDGGG
                     TFRARFGVEKDGESLLAADGSHTPGSDITTGYPEFDHAFLKKLGWWDDLTAEEKAEAE
                     GKNWKTDLSGGIQRVVMKVHGCMPFGNAKARAVVWNFPDGVPLHREPLFAPKPELVAK
                     WPTHADVKEKWRLPVLFKSVQDKSIADKDYEKFPLILTSGRLVEYEGGGEETRSNPWL
                     AELMQDNFAELNPKTAADRGIRHGEFVWVSTPSGAKIKVKALVTERVGADTVFIPFHF
                     SGWWQGQDMLGYYPEGAAPIVRGDAVNTATTYGYDIVTMMQETKTTLCQVAKA"
BASE COUNT          578 a         1362 c         1222 g          728 t
ORIGIN      
        1 gtacacagcg ctaacggaag taccgatcag cgcccctccg gcgagcgcca gcaggatctt
       61 cttcattgtt tttccttcat gtggggtgca cccgccggga ccgccccgcc gggcgcggat
      121 ttcgcgccac ccttgacctc gcggtaccag agttcgtggt gctccttggc ccaggcctca
      181 tcgacctgac ccgagcgcat cgcctggtag gcgccttcga cgccgatggt gcccaggtag
      241 atgtggccga gcgatgccgc catgaccacg agcgacgcca cggcgtgcca ggtccaggcg
      301 tcctgcatcg tgccgcgcgt ctgctcgaag ttcgggaaca gcagaatcag tcccgtcacg
      361 ctgacgatga tgcccatcgc cacgacgccg acccagaacc agctcttctc gcccgcgttg
      421 aagcggcccg agggcacgtg cgcacccgtg aacaggccgc ccgccttggc gacccactgc
      481 aggtccaccg ccttcgggaa attgtccttg atgtacagca cgatcatcat cagcaggccg
      541 aggatgaaca gcggaccgat gaagttgtgc aggttcttgg cgagcgcagt gagccacgcg
      601 aacagcgtat agccgatgac gggcagcagc acatgcttgc cgaacagcat gatcagaccc
      661 gagaccgcga gcacgacgaa cgtgatggcc acggtccagt gcgtcacgcg ctccaccgag
      721 ttgaagcgct ccagcatgcg cccggtgggc ttttcccgga gcttcagcgc gccccgggtg
      781 gtgaagaaag cggcgattcc cagcaccacg agcaccagca cccagccgcc atacaccgtc
      841 accggcccgt tacggatctg gcgccaggtc tccccggccg actgcaccag cacgcccatc
      901 tcgcggcccg gcaggctggt gtagccctgg ttgcccgagc gcacctcgcg ccagaccggc
      961 gcgttgttgc ccggctgcgt ctcgccgcgt tcctgttgcg cctgcgaatt gctcgcgctt
     1021 cccgcctgcg ccagcgccgt gcccgatcct gccatcgcga agatggcgag cgccaagccg
     1081 caggctctga gtagtgttgt catgccttcc tcctacttgg ggtggagaag cgcccgccta
     1141 ctgcttgtaa atgcggttgt cctcgttctg gcccatggcg cgctggttga tcgccgtctc
     1201 ccagctgctg cggtcgccct tgttccaggc actgccgcgg agctccgccg cgggcggggc
     1261 attgttccaa gcctgagtat ccggcttgcc ctggtacttg ccctgcttgt agaccgtcac
     1321 ctgctgcgcc tcgccgcatc ccgcggcgag gaacgctgcg gcgatcaccg cagcgcccag
     1381 cgcggcgcgg ttcacgactt gcccccggcg ggcggttgcg cgccggcctt gccgggcccg
     1441 tacgcggtgc cccagcccca gacttcggag cccttgccac ggcgcagcac gcgctcgcgg
     1501 tagatgtccg cgatcacgtc gccgtcaccg ccgagcaacg ccttggtcga gcacatctcg
     1561 gcacaggccg ggagcttgcc ctcggccagg cggttgcggc cgtacttctt gaactcggcc
     1621 tcggtgcggt cggcctccgg gccgccggcg cagaacgtgc acttgtccat cttgccgcgc
     1681 aggccgaagg cgcccgcctg cgggaactgc ggcgcgccga acgggcaggc atagaagcag
     1741 tagccgcagc cgatgcacag gtccttgtcg tgcagcacga cgccgtcctc ggtcttgtag
     1801 aagcagtcca ccgggcatac ggccatgcag ggcgcatccg agcagtgcat gcaggccacc
     1861 gagatcgact tctcggcgcc caccacgccg tcattgatgg tgacgacgcg gcgacggttc
     1921 acgccccagg gcacttcgtt ctcgttcttg caggcggtga cacagccgtt gcactcgatg
     1981 cagcgctcgg catcgcacag gaatttcatt ctcgccatgg cgttctcctt atgccttcgc
     2041 gacctgacag agggtcgtct tggtttcctg catcatggtg acgatgtcgt agccgtaggt
     2101 cgtcgccgta ttcaccgcat cgccgcgcac gatcggcgcg gcgccctccg ggtagtagcc
     2161 gagcatgtcc tgcccctgcc accagcccga gaagtggaac gggatgaaca cggtgtccgc
     2221 acccacccgc tcggtgacca gcgccttcac cttgatcttc gcgcccgagg gcgtgctcac
     2281 ccagacgaac tcgccgtgcc ggatgccgcg gtcggccgcg gtcttcggat tgagctcggc
     2341 gaagttgtcc tgcatcagct ccgccagcca cgggttggag cgcgtctcct cgccgccgcc
     2401 ttcgtactcc accaggcggc cggaggtgag gatgagcggg aacttctcgt agtccttgtc
     2461 ggcgatcgac ttgtcctgca ccgacttgaa cagcaccggc aggcgccact tttccttgac
     2521 gtccgcgtgc gtcggccact tcgccaccag ctccggcttg ggcgcgaaca gcggctcgcg
     2581 atgcagcggc acgccgtcgg ggaagttcca caccacggcg cgcgccttgg cgttgccgaa
     2641 gggcatgcag ccatgcacct tcatcaccac gcgctggatc ccgcccgaga ggtcggtctt
     2701 ccagttcttg ccttcggctt cggccttctc ttcggccgtg aggtcgtccc accagccgag
     2761 cttcttcagg aaggcgtggt cgaactccgg atagccggtg gtgatgtcgg agcccggcgt
     2821 gtgcgagccg tccgccgcga gcagcgattc accgtccttt tccacgccga agcgtgcgcg
     2881 gaaggtgccg ccgccgtcca tcacgtgctt ctcgatgttg tacaggttcg gcgagcccgg
     2941 gtgcttgagc ttgtcgttgc cccagcaggg ccacggcagg ccgaagtaat cgcccgtcag
     3001 gtcgtagccg gtcacggcgt ccttgccgcc cttggccttc agcgtcttca cgtcgaagac
     3061 gtgcatgttg cgcatgtgcg ccttgaggcg gtcgggggac tgcccggtgt agccgatggt
     3121 ccaggtgccg ctgttgaatt ccagcgtcgt gtcttccatc gagggctcgt ccaagccgcc
     3181 cttgcccttc accagcttga tgttctgctt gccgtccttc ttgccgagca gctggtcggc
     3241 gaagccgagc ttgcccgcga gctggtacat gatcatgtga tcggtacgcg actcgaacag
     3301 cggctcgatc acctgctcgc gccactggat cgagcggttc gaggccgtca cgctgcccga
     3361 ggtctcgaac tgcgtcgccg ccggcagcag gtagcagccc tccttcttcg ccatcgccat
     3421 catggcggcg gtggccgaag ggtatggatc gatgatcacc atcagctcga gcttcttcat
     3481 cgcctcgagc atgtcgaggc cgcggctttg cgagttcggc gcatggcccc agaacagcac
     3541 ggcgcgcagg ttggagtcct gccccatgtt ttccttcttc tcgacgatcg cgtcgtacca
     3601 gcgcgacacc gtgatgcccg gcttggtcat catcttctcg tcggcgtact gcttcttcag
     3661 ccagtccaga tcgatgttcc agacccgcgc ccagtgcgcg aacgagcccg gcgcaatgcc
     3721 gtagtagccc ggcagcgagt cggggttcgg cccgatgtcg gtcgccccct gcacgttgtc
     3781 gtggccgcgg tagatgttcg cgccgccgcc gccacgcccg gcattgccga gggcgagcat
     3841 caggatcgcg ctcgcgcgca cgatggcgtt gccgttggtg tgctgcgtct
//
LOCUS       DBNHKE010000027        24010 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_797444, whole genome shotgun sequence.
ACCESSION   DBNHKE010000027 DBNHKE010000000
VERSION     DBNHKE010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 24010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_797444"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="AC16O9_01465"
     CDS             complement(<1..1312)
                     /locus_tag="AC16O9_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HIR6515319.1"
                     /translation="MTDPMAWLVLLPLGWASLAFLLGPGRGALAAIIGLAAQLWLVAD
                     LAAAVSAAGARTHAVGGWGAPLGIDLAADGLSVVMLLLMQAVALPLAVYARAYFAPEP
                     DEARYFWPMTGFLVAAANALFLSADLFNLYVALELLGLAAVGLVAAGGGAPRLAAALR
                     YLLATLVGSGAYLLGVAALYGTYGTVSIATLTPLLAAGQAPALVWIAGGAMLAGLAVK
                     TALFPFHFWLPPAHGGAPAPVSALLSGLVVKASFYLILRLWLGPLGELASAAAGTAWL
                     LALLGSVAILWGSYQAIRAAHLKMLIAYSTVAQLGYLFLIFPLLAVPSAAQAGVMHVF
                     AHGFAKAAMFAAAGALIRATGQDTVAGLAGLAERLPLTLFAFGLAGLTLMGLPPSAGF
                     LAKWQLIEAALAQGYWFVAVVVLAGGLLAAFYVLRVLRQAFLSAP"
     gene            complement(1305..1610)
                     /locus_tag="AC16O9_01470"
     CDS             complement(1305..1610)
                     /locus_tag="AC16O9_01470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HIR6515320.1"
                     /translation="MTSGLIFACAGAAVFALGAAGVVLIAHLLRRVLAFNLMGSGAFL
                     VLVGLGQRAGVPDPVPQAMVLTGIVVAVAATALALALMRRLHAATGRSELPAAGDDD"
     gene            complement(1607..2266)
                     /locus_tag="AC16O9_01475"
     CDS             complement(1607..2266)
                     /locus_tag="AC16O9_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HIR6515321.1"
                     /translation="MKRALVVLAAGLLAVLLIAAMLQLPAPVVDLPAAVGARLGESGV
                     VHPVTAVLLNFRGYDTLLEIAVLLLALLGVLAAGGRETVAGEVRAAPAALRMLARLAA
                     PLMTVVAVYLLWAGALRPGGAFQAGAVLAAAAVLLHLVGLLPAWRSPAVPLRLALIAG
                     FLVFLAVAAALLASGALLQYPPARAGALILLIETALSVSLGLLLAGLLLFVSRGGAGG
                     R"
     gene            complement(2263..2517)
                     /locus_tag="AC16O9_01480"
     CDS             complement(2263..2517)
                     /locus_tag="AC16O9_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HIR6515322.1"
                     /translation="MSADLAFDLVLAGALLWSAWRTLATANLFQGVVQFIVFGLLMTL
                     AWARLAAPDIALAEAAIGAGLTGALLLDALGALRRGKDAP"
     gene            complement(2514..2825)
                     /locus_tag="AC16O9_01485"
     CDS             complement(2514..2825)
                     /locus_tag="AC16O9_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015302.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6515323.1"
                     /translation="MTDLAGVAGTVLLVAGAAFYCAGTVGLLRFPDVYSRLHALAKAD
                     NLGLGCVLAGLALQAETLAAALKLLLIWPLTLAASAAVSYAIAHRADALGVSPWRGAD
                     E"
     gene            complement(2818..3087)
                     /locus_tag="AC16O9_01490"
     CDS             complement(2818..3087)
                     /locus_tag="AC16O9_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrpF/PhaF family protein"
                     /protein_id="HIR6515324.1"
                     /translation="MTGFELPLALFLLINLAAALVAAVRGPTPADRMLTALLFGSVGV
                     GVLVLLARAMHLPALVDVALTLALLAAITGIAFARRGWRERRLDD"
     gene            complement(3084..3554)
                     /locus_tag="AC16O9_01495"
     CDS             complement(3084..3554)
                     /locus_tag="AC16O9_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR6515325.1"
                     /translation="MTRAILTRGFSFALLWWVLADGHAASWGLGAVAVVAATAASLRL
                     LPPGAQRIGAVALLRFLGFFLWSSLRSGVQVAALALRPRLDLAPIMLERRLALPPGAP
                     RLLMLNTLTLLPGTIGVSLEEARLRIHVLDARMPVAAELRALETHIARLFGTAP"
     gene            complement(3551..6202)
                     /locus_tag="AC16O9_01500"
     CDS             complement(3551..6202)
                     /locus_tag="AC16O9_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR6515326.1"
                     /translation="MPAREALQALQTGAHGLAPEEAARRLAAHGPNALPQARRRHPLM
                     RLLAQFNNALIYFLLAAALAAAVLAHFVDAAVIVAVVVVNAIIGFVQEGKAEQALAAM
                     HRMISPHATVLRAGARRSVAVAELVPGDIALLEAGDRVPADLRLLRARGLLIDEALLT
                     GESLAAEKTEDAVPAAAALGDRHGMAYSGTLVAAGQGTGVVVATGLATEIGHISALLQ
                     SVEQLTTPLLKQINRFAVRFTWFTLAAAVLVFAFAVGVRDFGWAEALMAVVALAVGAI
                     PEGLPAVITITLAIGVQRMAARKAVIRRLPAVETLGATSVICSDKTGTLTRNEMTARR
                     VVAGGHEVAVSGAGYVPEGALEAVGHDDDAAAVAAAVPLLRCGLLCNDAHLHRREESW
                     AVEGDPMEGALVALAMKAGLSAEHVRAEWPRLDEIPFDAARRYMATLHRGPAGETVAF
                     VKGAPEAVLGMAGAPEPERWAQRIAAAASAGERVLGFARKALPAGKERLRHADVKDGL
                     EFLGVVGFIDPPREEATHAVAECRSAGIEVKMITGDHARTAAAIARQLALAPDPQVLA
                     GAEIDAMDDAALVDAVRRTSVFARASPEHKLRIVRALQAEGAVVAMTGDGVNDAPSLK
                     QADVGVAMGIKGTEAAKEAAEMVLLDDNFASIVNAVHEGRTVFDNIRKVIAWTLPTNG
                     GEALTVISAIAFGFTLPVSAVQSLWINLVTAVTLGLVLAFEPAEPGVMRRPPRAQGAP
                     LLSPFLLWRVALVSVLFVGVALAVFFHALGRGYDLETARTLVVNVIVVLEIFYLFNVR
                     YLGTTSITLRGALGTPAVLIALGVVIVAQFAFTYAPFMHAWFGTRPVAFADGVLIVLL
                     GAAVMFLLEGEKWLLRRLGVAELSPPT"
     gene            complement(6232..7239)
                     /locus_tag="AC16O9_01505"
     CDS             complement(6232..7239)
                     /locus_tag="AC16O9_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6515327.1"
                     /translation="MQVKRRAGASGTMGAVKPHETVGGHMVASVLQARPGERVREVLA
                     RLAAEKPASLELVLATDAQGRLLGAAPLARVVGLRGDDPIEQALVRDFPRVRPNTDQE
                     RAASLALHHGVDALPVVDAAGRALGVMPAQALMQVLRREHVEDLHVLAGIQREAAQAR
                     HAIEDPPLRRVRHRLPWLLAGLAGAGVATAAMAGFEATLRAQVAVAFFVPGIVYLADA
                     IGTQSEAVAVRGLSLTRTGIARLLGGELRTGMAIGAVLALLSVLPVWFFFGSLRLAGA
                     VAVAIFAAGAVAAALGLALPWWLARAGRDPALGSGPLATVLQDILSLVVYLGVVKAFG
                     L"
     gene            7275..7793
                     /locus_tag="AC16O9_01510"
     CDS             7275..7793
                     /locus_tag="AC16O9_01510"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6515328.1"
                     /translation="MAVYPLHLVRQRRLADGRDVTIRPVRAGDERAEAQFLEGLSGES
                     QRLRFLDFVQAPGEKLVHFFTHIDYDRHMAFVCEAAGGALVGEARYVANADGRSCEFG
                     VVVADGWRHAGVAHLLMDALIRAAQAHGLETMEGLVLSENQDMLRFAKLLDFEINPDP
                     VKPALVRVRRKL"
     gene            7838..8497
                     /locus_tag="AC16O9_01515"
     CDS             7838..8497
                     /locus_tag="AC16O9_01515"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515329.1"
                     /translation="MGEAEVHALRGVDLAIGAGEFIVLLGPSGSGKSTLLNILGGLDV
                     PSAGDVRFLDHQLTGADEAELTRFRREHVGFVFQFYNLIPSLTARENVALVTDIAAEP
                     MPADEALGWVGLAERLDHFPSQLSGGEQQRVAIARAIAKRPQVLLCDEPTGALDYETG
                     KLVLEVIAKINTELGTTAMVITHNAAISGMADRVLRLGNGRIVGEEKPARKLSPAEIS
                     W"
     gene            8491..10854
                     /locus_tag="AC16O9_01520"
     CDS             8491..10854
                     /locus_tag="AC16O9_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6515330.1"
                     /translation="MVKALDRKLLRDLWSLKTQVVSIALVIACGIGGFIGSLSTHSSL
                     LWSRQNYYDTARFAHVFATAKRAPASLEAKLREIPGVAEAETRVVRDAQLSLPDVVPP
                     MIARLIGFDFAHPPGMNRLTLKQGRWPAPGATGEVVVNQRFFEARALQLGQPMSVLLN
                     GKLERLRLVGTALSPEHIYATRGGAMPDDEWFAVLWIDERALAAAFNMEGAFNSVLLR
                     LQHGASSETAVAALDRLLERYGGFGALGREDQASDKILSQEINQQKVFGTVLPAIFLL
                     VAAFILNVVLHRQVNAQRGEIAALKALGYDDRAIAWHYLKFASVIVLLGVALGLWLGH
                     WLGTALTGLYTDFFHFPRFHYRLEPWLALAAAGAALAAAAGGALAAIRGVVRLRAAEA
                     LRPSAPAEFRPLLIERLGYAELLTPAQRMIMRNLERKPLRAAITVVGVAASVAILIAG
                     VFWGDAIAWFMDVQFSQVQRAQVSVGFAEAVPRGVQHDLARLPGVKQVEVQRAVPVRL
                     RAGHRSYRTALTGLTDGAQLQRVLDAQLREAQLVPGAVLLTARLAERLGVAPGDTLEA
                     ELLEGNRIKTALRVGGTVDEMVGSNAWMRLEDLNRVAREGALVSGAGLLVDRAAERPL
                     LARLKEMPAAAVVIVTRTLVETFRETSAQNVLFFTAVLSAFAATIAVGVVYNNARIQL
                     AERAWELASLRVLGFTRAEVSVLLLGELALEIALAIPLGFVAGYWLAALLIAFMPHDV
                     LEFPLVIYPATYLLAGAVVAAAGVLSALIVRNRIDNLDLVGVLKTRE"
     gene            10851..12047
                     /locus_tag="AC16O9_01525"
     CDS             10851..12047
                     /locus_tag="AC16O9_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6515331.1"
                     /translation="MKTRNKILLAVAAAALAALVAWSLQPQPVAVELAAVTRGAFEQT
                     IVDDGKTRVRDRYVIAAPLSGRVERIRFEPGDPVRQGQVVALLTPTAPAFLDARTSRE
                     LQERVGAAQAQVARARAETAKVLAQRDQARADLERQAKLAAEGFVSPTAREQAALALR
                     TAERSVEAARFAEDAAGHDLAQARAALARYRSGDPTAKWEVTSPVNGSVLKVVQKSES
                     PVALGAPLLEVADARSLEAVVDVLSQDAVAVRPGMAARVELGQGVAPLPALVRRIEPA
                     AFTKISALGVEEQRVNVILDFAGPLDTIWTLGDAFRVEAHIVTYRAEDAVKVPVGALF
                     REGEGWAVFVAQDGAAAKRAVRVARRNGAEAMVEDGLAAGERVIVYPSDALRDGSAIE
                     ISSGRP"
     gene            12151..12903
                     /gene="otsB"
                     /locus_tag="AC16O9_01530"
     CDS             12151..12903
                     /gene="otsB"
                     /locus_tag="AC16O9_01530"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR6515332.1"
                     /translation="MTRATPQAILEAAGGRRLALFLDYDGTLTPIVERPEDAVLAEET
                     RAVLRRLARRHAVAIVSGRDLQDVRTRVGVEGLHYAGSHGFDIAGPGGGHVHGAARGA
                     EPRLAAAADELETRTAQLAGVQVERKRFAVAVHYRRARKSDAPRVEAAVDRALARHPG
                     LRKTGGKMIFELRPDVDWDKGRAVRWLIDDLELQDAVPVYIGDDLTDEDAFRALAGRG
                     VGIAVQESPQPTAATYTLRDPDEVRALLAGLA"
     gene            12900..15296
                     /locus_tag="AC16O9_01535"
     CDS             12900..15296
                     /locus_tag="AC16O9_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIR6515333.1"
                     /translation="MSWSLDYRDFKPAEERLREALCTLGNGYFATRGAAPEAVASEVH
                     YPGTYLAGGYDRQPTEIAGRRIENEDLVNLPNWLALTFRIDGADWFDLKAVEILEFRQ
                     TLDFSCGVLERHVRFRDAAGRHTRLDERRLVHMVEPHLAALETVLTAEDWSGPLELLS
                     ALDARVENAGVARYRDLARKHLEPLEARAAGGDMVFLRVRTLQSRLEVALAARTRLDR
                     AAQTRARIEPGYAGQHLHLEMKKGEPLRAEKVVALHTSRDRAVSECGEAALESCARAP
                     GFAALAAGHGLAWRHLWQRFDIALEDGGERTQRILRLHLFHLLQTASPNSKMLDAGVP
                     ARGLHGEAYRGHIFWDELFIFPLLNFRLPEITRALLLYRYHRLAAARENARAAGYRGA
                     MFPWQSGSSGREESQVVHLNPRSGRWIPDHTYLQRHVNAAIAYNVWQYWQVTRDREFL
                     AFYGAEIFLEIARFWGSYATFNETRRRYEILGVVGPDEFHTADPDAGRPGLDNHAYTS
                     VMAAWVLARAFDVLAALPDERRRELRETLALGDEELARWDDASRRMHVAFHGAGLISQ
                     FEGYEKLAELDWARYRAEYRDISRLDRILEAEGQDVNRYQCSKQADVLMLFYLFSAEE
                     LAELFGRLGYPFPPDTIPKNVAYYEARTTHGSTLSRVVNSWVLARSDRAGSWRQFLEA
                     LESDVGDIQGGTTPEGIHLGAMAGTVDLVQRGYTGIEARGDALWLNPRLPEELARLRL
                     TVRYRGLTLDLEVRQDTLEVHARASNVAPIALGIVDAVHRVAAGEKRAFALRREAPLA
                     "
     gene            15332..15796
                     /locus_tag="AC16O9_01540"
     CDS             15332..15796
                     /locus_tag="AC16O9_01540"
                     /inference="COORDINATES: protein motif:HMM:NF037928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIR6515334.1"
                     /translation="MTLMPPMWIVAADSSQCRIFAAHAPDGALQELETLSHPEARLQE
                     QALVSDSPGRAFDSKGAGRHAMEVEVSAKKHEAMMFAGRIAARLDAGRVRRDFDKLAL
                     VAAPEFLGLLRGKLGPQLRALVVHEVDTNPSGMKADAIRARLPARLFPAPPV"
     gene            15879..16271
                     /locus_tag="AC16O9_01545"
     CDS             15879..16271
                     /locus_tag="AC16O9_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR6515335.1"
                     /translation="MAPNRREMIELARAIEERREALATEIRREVARARNEPFAEVAGT
                     VHDTGDEALADLVADVDNAEVTRDLAELRALEGARARIAGGSYGVCADCGADIPVARL
                     RAQLAALRCIACQERQEKGYAGGARAKL"
     gene            complement(16268..17974)
                     /gene="polX"
                     /locus_tag="AC16O9_01550"
     CDS             complement(16268..17974)
                     /gene="polX"
                     /locus_tag="AC16O9_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR6515336.1"
                     /translation="MAVHNADIAAAFEEIADLLELGDDNPFRVRAYRNAARIVGGLSL
                     DLAAALAAGRELPKLPGIGADLDGKIHEIAASGSCKLLERLRKKFPAGITALLRLPGL
                     GPKRLRALHAQRISSLEALERAARAGRLAQIPGFGEKTAQRLLDAAAAHAKKKPRVKL
                     AVAAQYAEPFAKYLGATVAGSYRRMKETVGDLDFLVAAAKPAPAVKRFVTYGEVKKVL
                     AQGSTRASAVLSSGIQCDLRVVPTASFGAALHYFTGSKAHNIAVRKLGIARGLKINEY
                     GVWKANKRVAGETELSVYRAVGLPYIEPELREEAGELEAARRGELPALLELKNLKGDL
                     HAHTRATDGHNTLREMALAAKAHGLSYLAITEHSRKLTLAHGLDPQRLAKQIAEIEEL
                     NGKLSGITLLKGIEVDILEDGTLDLPDSILEKLDLVVGAVHSKFDLPRARQTERIVKA
                     MRNPLFSILAHPTGRLIEQRAPYDVDMLALVRAAKKSGVALELNAHPDRLDLTDTHCR
                     MAKDEGVLVAVSSDAHDVDGFDVLRYGVGQARRGWLETKDVLNARPLAELRKALARRS
                     AA"
     gene            complement(17990..18271)
                     /locus_tag="AC16O9_01555"
     CDS             complement(17990..18271)
                     /locus_tag="AC16O9_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515337.1"
                     /translation="MTQPIPETPPEYDRARIIERPDGVYWQDKESGREYGPFPTLLEA
                     VQDMELGGDRSLESGETLEEAEEEIGISDYLDPDTGQPAEDGVPRLEDH"
     gene            complement(18430..18753)
                     /locus_tag="AC16O9_01560"
     CDS             complement(18430..18753)
                     /locus_tag="AC16O9_01560"
                     /inference="COORDINATES: protein motif:HMM:NF052504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sll1863 family stress response protein"
                     /protein_id="HIR6515338.1"
                     /translation="MARKRRTAATKRKGERWLNLHKEVRAVGKRLDWLMAEARKAEKS
                     ARATAVRDLQLLKRKQAAANRALAKLGRQSAAASAPLVAGMQKAWHDMELAMRQASKR
                     FRQTS"
     gene            18884..19216
                     /gene="hpf"
                     /locus_tag="AC16O9_01565"
     CDS             18884..19216
                     /gene="hpf"
                     /locus_tag="AC16O9_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6515339.1"
                     /translation="MQLPVQITYHGMESSAALDNAVREKVAKLEQYHPRITSCRVVIE
                     QPAQRKRKGRQFVVRVDLTAPGNEIALNHDHAEDVYVALRDAFDAARRRLEVRARTRR
                     AELKRHER"
     gene            19220..19675
                     /locus_tag="AC16O9_01570"
     CDS             19220..19675
                     /locus_tag="AC16O9_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6515340.1"
                     /translation="MKRLSRPAADRKIAGVCAGLGEYFDLDPVFFRLFFLFSILFGGL
                     GAVAYIMLWILVPAGEGARPAPGDGKRLRLSGKERMIAGVCGGLGEFFELDPVLFRAV
                     FLVLAVIGGLGILLYLVLWLLIPGPATAAGAPPSPGRARQARSAHRARE"
     gene            complement(19587..20687)
                     /locus_tag="AC16O9_01575"
     CDS             complement(19587..20687)
                     /locus_tag="AC16O9_01575"
                     /inference="COORDINATES: protein motif:HMM:NF053377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8173 domain-containing protein"
                     /protein_id="HIR6515341.1"
                     /translation="MIAVRAMSLLLLCAPLAGATADERGEHLYLRQQGADTFVAGDSA
                     RVSQPVAGDLLAAGGELRLAAPVAGDIVAVGGALRLDGSGSQNLYAAGGNIAVEGTLA
                     RNARVAGGNLSVGPQARISGNASLAGGRVEMLGAVGGYLQAAGGRVLIDGRVGGDVEA
                     VAGELELGPNARIGGRLRYHGGAPLRQDAAARVEGGIERLEMPVRRVAERSPAPAVFG
                     VWTLGLMVMAAVLVLALPGLSSRVSAAARARLGWSLLAGFLALAAVPLAVVILLATVV
                     GIPLALAGVLGYLALLLLGYVAAGIALGDAALARWWAARAAHRGWRALFAALGVLVLG
                     VAALIPWLGVLIALAALVLGMGALLLQSRAPG"
     gene            complement(20737..20979)
                     /locus_tag="AC16O9_01580"
     CDS             complement(20737..20979)
                     /locus_tag="AC16O9_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515342.1"
                     /translation="MKDPMPQVGAPDPEAKNSPIVDHSEYDDRDAMFDVELEAGACYF
                     NGQVYAIGDTVRSGSDVLECAPRGVWVRKGVRRDEV"
     gene            complement(21042..21563)
                     /locus_tag="AC16O9_01585"
     CDS             complement(21042..21563)
                     /locus_tag="AC16O9_01585"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR6515343.1"
                     /translation="MSFAVWLTGLSGSGKSAIARELLAQLHARGVEAAVLESDVLRTQ
                     LTPFATYAPQERDFFYAALVQLGSDVARRGRPVLFDATANLRVYRDAARAKIARFAEV
                     FVDTPLEVCAARDTKGLYRAAREGRSATLPGAQAAYEPPAAPELVVHGDRGTPAESAT
                     AIAAWLEKRAWLD"
     gene            complement(21560..22639)
                     /locus_tag="AC16O9_01590"
     CDS             complement(21560..22639)
                     /locus_tag="AC16O9_01590"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6515344.1"
                     /translation="MSASPFTPAALRDYLRALHGEEVTNVRLTPLGGGRQGDKGYGYG
                     VPLRVDYERAGQAQRAVLETVRPGPFGHEHMADRAQLLLWAHGAFNRLPRHVPSLDVG
                     AVRAGGALEPLGSAEELFMLAQFVEGHEYADDLLRLRGGADALPQDFARADALCDYLI
                     EIHRRAGPDPGLYVRRVRELLGHGECIFGVADSYAEGPYLESVREIERRCLEWRWRLK
                     PRAHRLRQVHGDFHPWNLLFREGTDFTVLDRSRGEWGEPADDVTCLALNYLFFSLQRS
                     GRLEGDFERLWRGFWGRYLEGSGDLEMLEVAAPFFAFRALVMANPLWYPALDARLRRK
                     MLDFALNVLAAPRFDPARANAYCGA"
     gene            22714..>24010
                     /locus_tag="AC16O9_01595"
     CDS             22714..>24010
                     /locus_tag="AC16O9_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6515345.1"
                     /translation="MEKLTGRNAFLRLLVEEGVTHLFGNPGTTELAIMEAVPDFPQLR
                     YVLGLHESVVVAMADGYARASNRLAAANVHVAPGLGNAMGALYNAQFSGSPIILTAGQ
                     QEQGHGLGEPLLYAPLVPVATPFVKWAVEATRVEDLPRIIRRAAKVALTPPTGPVFIS
                     LPGDVLEQQSEIELGAPTRIDARTRPSDEALAELARRLLDAKNPVIVAGHELSIHDAF
                     AEATELAELLGAAVYQQTVPYAAQYPTGHRAYLGALTRVQKSVRAALEPFDLLLCLGA
                     DLLRMSVYSPLDPLPAGLPVIHVSERDWEMGKNHPTAMALHANVKETLRALLPLLRTR
                     RGAEGAAAAERRLAALAPRNWRAQRDKARLAALEAAEEKPVDPRFLMLRFAEALPADA
                     VVVEEALTSSLSLPAVLEQRDAKSFYGLASGGLGFALPGA"
BASE COUNT         3518 a         8853 c         8503 g         3136 t
ORIGIN      
        1 ccggcgccga gagaaacgcc tggcgaagga cgcgcagcac gtaaaaggcc gccagcagcc
       61 cgccggccag cacgaccacc gcgacgaacc agtacccctg cgcgagcgcg gcctcgatca
      121 gctgccactt ggcgagaaag cccgcggacg gcggcaggcc catcagcgtg agacccgcca
      181 ggccgaaggc gaacagcgtg agcggcaggc gctcggccag gcccgcgagc cccgcgacgg
      241 tgtcctgccc ggtcgcgcgg atcagcgcgc ccgccgccgc gaacatcgcg gccttggcga
      301 agccgtgcgc gaacacgtgc attacgccgg cctgcgcggc gctcggcacc gcgagcagcg
      361 gaaagatcag gaacaggtag ccgagctgcg cgacggtcga ataggcgatc agcatcttca
      421 ggtgcgcggc gcggatggcc tggtacgaac cccagagaat cgcgacgctg cccaaaagcg
      481 cgagcagcca cgcggtgccg gcggcggccg acgcgagctc gccgagcggg ccgagccaca
      541 gccgcaggat gaggtagaag gaggccttga ccaccagtcc ggagagcagc gccgataccg
      601 gcgccggcgc gccgccgtgc gccggcggca gccagaagtg aaagggaaac agcgccgtct
      661 tcaccgccag cccggcgagc atcgcgccgc cggcgatcca cacgagcgcc ggcgcctggc
      721 ccgccgcgag cagcggcgtg agcgtggcga tcgacaccgt cccgtaggtg ccgtagagcg
      781 cggcgacacc cagcaggtaa gccccggagc cgaccagcgt tgcgagcagg tagcgcagcg
      841 cggccgcgag ccggggcgcg ccgccgcccg ccgccaccag gccgaccgcc gcgagcccga
      901 gcagctcgag cgcgacgtac aggttgaaca ggtcggccga caggaacagc gcgttcgccg
      961 cggcgacgag gaacccggtc atcggccaga aatagcgcgc ctcgtcgggc tcgggcgcga
     1021 agtaggctcg cgcgtagacc gccagcggca gggcgacggc ctgcatgagc agcagcatga
     1081 cgaccgacag gccgtcggcg gcgaggtcga tgccgagcgg cgcgccccag ccgcccacgg
     1141 cgtgggtacg cgcgccggcg gcgctgaccg cggcggcgag atccgcgacc agccacagct
     1201 gcgccgcgag gccgatgatc gccgcaaggg caccgcgccc gggcccgagg aggaaggcga
     1261 ggctcgccca gccgagcggc agcagcacga gccacgccat gggatcagtc atcgtcgccg
     1321 gccgccggca gttccgagcg accggtggcc gcgtgcagcc ggcgcatgag cgccagcgcg
     1381 agcgccgtgg ccgcgactgc gacgacgatg ccggtgagca ccatcgcctg cggcacgggg
     1441 tccgggacgc ccgcgcgctg cccgaggccg accaggacga gaaacgcgcc gctgcccatc
     1501 aggttgaagg ccagcacgcg ccgcaggagg tgcgcgatca gcaccacgcc cgccgccccc
     1561 agcgcgaaca cggccgcgcc ggcgcaggcg aagatcagcc cgctggtcat cggccgcccg
     1621 cgccgccgcg cgagacgaac agcagcagcc ccgcgagcag caggccgagc gacaccgaca
     1681 gcgcggtctc gatcagcagg atcagcgcgc cggcccgcgc cggcggatac tgcagcagcg
     1741 cgccactcgc cagcagcgcg gcagccacgg cgaggaacac gaggaagccg gcgatgagcg
     1801 cgagccgcag cggcaccgcg gggctgcgcc aggcgggcag cagccccacc agatggagca
     1861 ggaccgcggc ggccgccagc acggcgccgg cctggaacgc gccgccgggc cgcagggcgc
     1921 ccgcccacaa caggtagacc gcgacgacgg tcatcagcgg cgccgcgagc cgcgccagca
     1981 tgcgcagcgc cgcgggcgcg gcgcgcacct cgcctgctac cgtttcgcgg ccgcccgccg
     2041 cgagcacccc gagcagcgca agcagcagca cggcgatctc gagcagcgtg tcgtagccgc
     2101 ggaagttgag cagcacggcg gtgaccgggt ggacgacccc gctctcgccg agtcgcgcgc
     2161 cgaccgccgc gggcagatcc accactggcg ccggcagctg gagcatcgcc gcgatgagca
     2221 gcacggcgag caggccggcg gcgaggacga cgagcgcacg cttcacggcg cgtccttgcc
     2281 gcggcgcagc gcgccgagcg catcgagcag gagcgcgccg gtgagacccg cgccgatcgc
     2341 ggcctcggcg agcgcgatgt ccggcgccgc gaggcgcgcc caggcgagcg tcatcagcag
     2401 gccgaagacg atgaactgca cgacgccctg gaacaggttc gccgtggcga gcgtgcgcca
     2461 cgcgctccac agcagcgcgc cggccagcac gaggtcgaac gcgaggtccg cgctcattcg
     2521 tccgccccgc gccacggcga cacgccgagc gcatcggcgc ggtgcgcgat cgcgtagctc
     2581 accgcggcgc tcgccgcgag cgtcagcggc cagatgagga gcagcttgag cgcagcggcg
     2641 agggtctcgg cctgcagcgc gagccccgcc agcacgcagc ccagccccag gttgtcggcc
     2701 ttggcgagcg catgcagccg cgagtagacg tccggaaagc gcagcaggcc caccgtcccg
     2761 gcgcagtaga acgccgcgcc ggccaccagc agcaccgtgc ccgccacgcc cgcgaggtca
     2821 gtcatcgagc ctcctttcgc gccagccgcg ccgcgcgaag gcgatgccgg tgatcgccgc
     2881 cagcagcgcg agcgtcagcg ccacgtcgac cagcgccggc aggtgcatcg cgcgcgcgag
     2941 cagcaccagc acgcccacgc ccacgctgcc gaaaagcagc gccgtcagca tgcggtcggc
     3001 cggcgtcggc ccgcgtaccg cagcgaccag cgccgccgcc aggttgatca gcaggaacag
     3061 cgccagcggc agctcgaatc cggtcacggc gccgtcccga acagccgcgc gatgtgcgtt
     3121 tcgagggccc gcagctcggc cgccaccggc atgcgcgcgt cgagcacatg gatcctgagc
     3181 cgcgcctctt ccaggctcac gccgatggtt cccggcagca gcgtgagcgt gttgagcatc
     3241 agcaggcgcg gcgcccccgg cgggagggca aggcgccgct cgagcatgat cggcgcgaga
     3301 tcgagccggg gacgcagcgc gagcgccgcc acttggacgc cgctgcgcag cgagctccac
     3361 aggaaaaagc cgagaaagcg cagcagggcc actgcgccga tgcgctgcgc acccggcggc
     3421 agcagccgca ggctcgccgc ggtggccgcc accaccgcca ccgcgcccaa tccccagctc
     3481 gcggcgtgcc cgtccgccag cacccaccag agcagggcga acgaaaagcc gcgcgtcagg
     3541 atcgcgcgcg tcatgtcggc gggctcaact ccgccacccc cagccgccgc aacagccact
     3601 tctcgccctc cagcaggaac atcacggctg cgcccagcaa gacgatcagc acgccgtcgg
     3661 cgaacgccac cggccgcgtg ccgaaccacg cgtgcatgaa cggcgcataa gtgaaggcga
     3721 attgcgccac gatcaccacg ccgagcgcaa tgagcaccgc cggcgtgccg agcgcgccgc
     3781 gcagcgtgat cgaggtcgtg cccaggtagc gcacgttgaa cagatagaag atctccagca
     3841 cgacgatgac gttgaccacc agcgtgcgcg cggtttccag gtcgtagccg cggccgagcg
     3901 cgtggaagaa caccgccagc gccacgccga cgaacagcac cgacaccagg gccacgcgcc
     3961 acagcaggaa cggcgagagc agcggcgcgc cctgcgcgcg cggcggccgg cgcatgacgc
     4021 cgggctcggc gggctcgaag gcgagcacca gcccaagggt caccgcggtg acgaggttga
     4081 tccacaggct ctgcaccgcg ctcaccggca gcgtgaagcc gaaggcgatc gccgagatca
     4141 cggtgagcgc ctcgccgccg ttggtgggca gggtccaggc gatcaccttg cggatgttgt
     4201 cgaacacggt gcggccctcg tgcacggcgt tgacgatgga ggcgaagttg tcgtcgagca
     4261 gcaccatctc ggcggcctcc ttggccgcct cggttccctt gattcccatg gcgacgccga
     4321 cgtcggcctg tttcagcgag ggcgcgtcgt tcacgccgtc gccggtcatg gcgaccaccg
     4381 cgccttcggc ctgcagcgcg cgcacgatgc gcagcttgtg ctcggggctc gcgcgcgcga
     4441 acacgctggt gcggcgcacc gcgtcgacca gtgcggcatc gtccatggcg tcgatctcgg
     4501 cgccggcgag cacctgcggg tcgggcgcga gcgcgagctg gcgcgcgatc gcggccgcgg
     4561 tgcgcgcgtg atcgccggtg atcatcttca cttcgatgcc cgccgagcgg cactcggcca
     4621 ccgcgtgcgt cgcctcctcg cgcggcggat cgatgaaacc gacgacgccg aggaactcga
     4681 ggccgtcctt gacgtcggcg tggcgcaggc gctctttgcc cgccggcaag gccttgcggg
     4741 cgaagccgag cacgcgctcg ccggccgacg cggcggccgc gatgcgctgc gcccagcgct
     4801 ccggctcggg cgcgccggcc atcccgagaa cggcttccgg cgcgcctttc acgaacgcga
     4861 ccgtctcgcc cgccgggccg cggtgcagcg tcgccatgta gcggcgcgcg gcgtcgaacg
     4921 ggatctcgtc caggcgcggc cactcggcgc gcacgtgctc cgcgctgagc cccgccttca
     4981 tggcgagcgc caccagggcg ccctccatcg gatcgccctc gaccgcccag ctttcttcgc
     5041 ggcggtgcag gtgcgcgtcg ttgcacagca gcccgcagcg cagcagcggt accgcggcgg
     5101 caaccgccgc cgcgtcgtcg tcgtggccca ccgcctcgag cgcgccttcg ggcacgtagc
     5161 ctgcgccgct caccgcgacc tcgtgcccgc ccgcgaccac gcgccgcgcg gtcatctcgt
     5221 tgcgggtgag cgtgccggtc ttgtccgagc agatcaccga ggtggcgccg agcgtctcca
     5281 cggcgggcag gcgccggatc acggcctttc tcgccgccat gcgctgcacg ccgatcgcca
     5341 gcgtgatggt gatcaccgcg ggcaggcctt cggggatggc gccgacggca agcgccacca
     5401 ccgccatcag cgcctcggcc cagccgaagt cgcgcacgcc caccgcgaag gcgaacacca
     5461 gcaccgcggc ggccagcgtg aaccaggtga agcgcaccgc gaagcggttg atctgcttga
     5521 gcagcggcgt agtgagctgc tcgacgcttt gcaggagcgc gctgatatgc ccgatttcgg
     5581 tggcgaggcc cgtggccacc accacgcccg tgccctgccc ggcggccacc agcgtgcccg
     5641 agtaggccat gccatggcgg tcgccgagcg cggctgcggc cggcacggcg tcctcggtct
     5701 tctcggcggc gagcgactcg cccgtgagca gcgcttcatc gatgagcagc ccgcgcgcgc
     5761 gcagcagccg caggtccgcg ggcacgcggt cgcccgcctc gagcagcgcg atgtcgccgg
     5821 gcacgagctc ggccaccgcg acgctgcgcc gcgcgccggc gcgcagcacc gtcgcgtgcg
     5881 gcgagatcat gcggtgcatc gcggccagcg cctgttccgc cttgccttcc tgcacgaagc
     5941 cgatgatggc gttcaccacg accaccgcga cgatgaccgc agcatcgacg aaatgcgcga
     6001 gcaccgcggc ggcaagcgcc gccgcgagca ggaagtagat caacgcattg ttgaattgcg
     6061 cgagcaagcg catcaacggg tggcggcgcc tggcctgcgg cagcgcgttc gggccgtgcg
     6121 cggcgagccg gcgcgcggcc tcctccggcg caagtccgtg cgcgcccgtc tgcagcgcct
     6181 gcagcgcctc gcgcgcgggc atcgcgtgcc agtcgcgtgc cgcctgcgcg ttcaaagccc
     6241 gaatgccttc acgacaccga ggtagaccac gaggctgagg atgtcctgca gcacggtggc
     6301 gagcggcccg ctgccgagcg cagggtcgcg ccctgcgcgt gcgagccacc agggcagggc
     6361 gagcccgagc gccgcggcaa cggcgcctgc ggcgaagatc gccaccgcca ccgcgcccgc
     6421 cagccgcagg ctgccgaaga agaaccacac cgggagcacg ctgagcagcg cgagcacggc
     6481 gccgatcgcc atgccggtgc gcagctcgcc gccaagcagg cgcgcgatgc ccgtgcgcgt
     6541 gagcgagagc ccgcgcacgg cgaccgcctc gctctgcgtg ccgatcgcat ccgccaggta
     6601 gacgatcccc ggcacgaaga aggcgaccgc cacctgcgct cgaagggtcg cctcgaaccc
     6661 cgccatcgcc gcggtcgcca cgccggcgcc agcgagcccc gcgagcagcc acggcagccg
     6721 atgacgcacg cggcgcagcg gcgggtcctc gatggcatgg cgggcctgcg cggcctcgcg
     6781 ctggatgccc gccagcacgt gcaggtcctc gacatgctcc cggcgcagca cctgcatcag
     6841 cgcctgcgcg ggcatcacgc cgagcgcgcg gccggccgcg tccaccactg gcagcgcgtc
     6901 gacgccgtgg tgcagcgcga gcgaggcggc gcgctcctgg tcggtgttcg ggcgcacgcg
     6961 aggaaagtcg cgcacgagcg cctgttcgat cgggtcgtcg ccgcgcagcc cgacgacgcg
     7021 ggcgagcggc gccgcgccga gcagacgtcc ctgcgcgtcg gtcgccagca ccagctccag
     7081 gctggccggc ttctccgcgg cgaggcgcgc cagcacctcg cgcacacgct ctccgggccg
     7141 cgcctgcagc accgatgcga ccatgtgccc gcctacggtc tcatgcggct tcaccgcgcc
     7201 catcgtgccg ctggcgcccg cgcgccgctt gacttgcatc aaccaccgct gcggtgtgcg
     7261 gccgacaatt tcgcatggcc gtgtatccgc tgcacctcgt gcgccagcgc cggcttgccg
     7321 acgggcgtga tgtgacgatc cgcccggtac gcgccggcga cgagcgtgcc gaagcgcaat
     7381 tcctcgaggg tctgtccggc gagagccagc gcctcaggtt cctcgacttc gtgcaggcgc
     7441 cgggcgagaa gctggtgcat ttcttcacgc acatcgacta cgatcgccac atggccttcg
     7501 tctgcgaagc cgccggcggc gcgctggtcg gcgaggcgcg ctacgtggcc aacgccgatg
     7561 gccgcagctg cgaattcggc gtggtggtgg ccgacggctg gcgccacgca ggcgtggcac
     7621 atctgctcat ggacgcgctg atccgcgccg cgcaggcgca cgggctcgag accatggagg
     7681 gcctggtgct gagcgagaac caggacatgc tgcgcttcgc gaaactgctc gacttcgaga
     7741 tcaaccccga tcccgtgaag cccgcgctgg tgcgcgtgag gaggaagctt tgagcgagcc
     7801 ggtgttccag gctcgcggcc tgaccaagac ttaccacatg ggcgaagcgg aggtgcacgc
     7861 gctgcgcggc gtcgatctgg cgatcggcgc gggcgagttc atcgtgctgc ttgggccctc
     7921 gggcagcggc aagtccacgc tgctcaacat cctcggcggc ctcgacgttc cgagcgcggg
     7981 cgacgtgcgc tttctcgatc accagctcac gggcgcggac gaggccgagc tgacgcgctt
     8041 ccggcgcgag cacgtgggtt tcgtgttcca gttctacaac ttgatcccga gtctcaccgc
     8101 gcgcgagaac gtggcgctgg tgaccgacat cgccgccgag ccgatgccgg ccgacgaggc
     8161 gctcggctgg gtgggcctcg ccgagcggct ggaccacttc ccctcgcagc tctcgggcgg
     8221 cgagcagcag cgtgtggcaa tcgcgcgcgc catcgccaag cgcccgcagg tgctgctgtg
     8281 cgacgagccc accggcgcgc tcgactatga gaccggcaag ctggtgctcg aggtcatcgc
     8341 caagatcaac accgagctcg gcaccacggc gatggtgatc acgcacaacg cggcgatttc
     8401 gggcatggcc gaccgcgtgc tccggctcgg caacggaagg atcgtgggcg aggagaagcc
     8461 cgcgcgcaaa ctcagtccgg cggagatctc gtggtaaagg cgctcgaccg caagctgctc
     8521 cgggacctgt ggagcctgaa gacccaggtg gtctcgatcg cgctggtgat cgcctgcggc
     8581 atcggcggct tcatcggctc gctctccacg cactcgtcgc tcctgtggtc gcgccagaac
     8641 tactacgaca cggcgcgctt cgcgcacgtg ttcgccaccg ccaaacgcgc gccggcctcg
     8701 ctcgaagcga agctgcgcga aattcccggc gtggccgaag ccgagacgcg cgtggtgcgc
     8761 gacgcgcagc tgtcgctgcc ggacgtggtg ccgccgatga tcgcgcggct gatcggcttc
     8821 gacttcgcgc accctccggg catgaaccgg ctgacgctga agcaggggcg ctggcccgca
     8881 cccggcgcga cgggcgaggt ggtggtcaac cagcgcttct tcgaagcgcg cgcgctgcag
     8941 cttggccagc cgatgagcgt gctgctcaac ggcaagctcg agcgcctcag gctcgtcggc
     9001 accgcgctca gccccgagca catctacgcc acgcgcggcg gcgccatgcc cgacgacgag
     9061 tggttcgcgg tgctgtggat cgacgagcgc gcgcttgccg cggccttcaa catggagggc
     9121 gcgttcaact cggtgctgct caggttgcag catggcgcat cgagcgagac cgcggtggcg
     9181 gcgctcgatc gcctgctcga gcgctacggc ggcttcggcg cgctcgggcg cgaagaccag
     9241 gcctccgaca agatcctctc gcaggagatc aaccagcaga aggtgttcgg caccgtgctg
     9301 ccggcgatct tcctgctcgt ggcggccttc atcctcaacg tggtgctgca ccggcaggtg
     9361 aacgcgcagc gcggggagat cgcggcgctg aaggcgctcg gctacgacga ccgcgccatc
     9421 gcctggcact acctcaagtt cgcctcggtg atcgtgctgc tcggggtggc gctcggcctg
     9481 tggctcggcc actggctcgg caccgcgctc accggcctgt acaccgactt cttccacttt
     9541 ccccgcttcc actaccggct cgagccctgg ctcgcgctcg ccgccgccgg tgccgcgttg
     9601 gccgcagcgg ccggcggggc gctcgccgcg atccgcggcg tggtgcgcct gcgcgctgcc
     9661 gaggcgctgc gaccgagcgc cccggccgag ttccggccgc tgctcatcga gcggctcggc
     9721 tacgccgagc tgctcacccc ggcgcagcgc atgatcatgc gcaacctgga aagaaagccg
     9781 ctgcgcgccg cgatcaccgt cgttggcgtc gcggcgtcgg tggcgatcct catcgccggc
     9841 gtattctggg gcgacgccat cgcgtggttc atggacgtgc agttcagcca ggtgcagcgc
     9901 gcacaggtga gcgtcggctt cgccgaggcc gtgccgcgcg gcgtgcagca cgaccttgcg
     9961 cgtctgcccg gcgtgaagca ggtcgaggtg cagcgcgccg tccccgtgcg cctgcgtgcc
    10021 ggccaccgca gctaccgcac cgcgctcacc ggcctcacgg acggcgccca actgcagcgc
    10081 gtgctggacg cgcagctgcg cgaggcgcag ctcgtgcccg gcgcggtgct gctcaccgcg
    10141 cggcttgccg agcgcctggg cgtggcgccc ggggacaccc tcgaggcaga gctgctggaa
    10201 ggcaatcgca tcaagaccgc gctgcgcgtc ggcggtaccg tcgacgaaat ggtgggctcg
    10261 aatgcctgga tgcgcctgga ggatctgaac cgcgtggcgc gcgagggcgc gctcgtctcc
    10321 ggcgcgggtc tgctcgtcga ccgcgccgcc gagcgcccgc tgctcgcgcg cctgaaggag
    10381 atgccggcgg cggccgtggt gatcgtcacg cgcacgctgg tcgagacctt ccgcgagacc
    10441 agcgcgcaga acgtgctttt cttcaccgcg gtgctctccg ccttcgccgc caccatcgcg
    10501 gtgggcgtgg tgtacaacaa cgcgcgcatt cagctcgccg agcgcgcctg ggagctcgcc
    10561 agcctgcgcg tgctcggctt cacgcgcgcc gaggtgtcgg tgctgctgct cggcgagctc
    10621 gcgctcgaga tcgcgctcgc catcccgctc ggcttcgtcg ccggctactg gctggcggcg
    10681 ctgctcatcg ccttcatgcc gcacgacgta ctggagttcc cgctcgtcat ctatcccgca
    10741 acctacctgc tcgcgggcgc cgtggtggcc gccgccggcg tgctgagcgc gctcatcgtg
    10801 cgcaatcgga tcgataatct ggacctggtc ggagtgctga agacgcgcga atgaagaccc
    10861 ggaacaagat cctgctcgcc gtggccgcgg ctgcgctcgc ggcgctcgtc gcctggtcgc
    10921 tgcagccgca gccggtcgcc gtggagctcg ccgcggtgac ccgcggcgcg ttcgagcaga
    10981 ccatcgtcga cgacggcaag acgcgcgtgc gcgaccgcta cgtgatcgcc gcgccgctct
    11041 ccggccgggt ggagcgcatc cgcttcgagc cgggcgaccc ggtgcgccag ggacaggtgg
    11101 tggcgctgct gaccccgacc gcgcccgcct tcctcgacgc gcgcacctcg cgcgagctgc
    11161 aggagcgcgt gggcgccgcg caggcgcagg tggcgcgcgc gcgcgcggag acggcgaagg
    11221 tgctggcgca gcgcgaccag gcgcgcgccg acctcgagcg ccaggcgaag ctcgccgccg
    11281 agggcttcgt ctcgcccacc gcgcgcgagc aggcggcgct cgcgctgcgc accgcggagc
    11341 gctcggtgga agccgcgcgc ttcgccgagg atgccgcggg ccacgacctg gcgcaggcgc
    11401 gcgcggcgct tgcgcgctat cgcagcggcg acccgaccgc aaagtgggag gtaacctcgc
    11461 cggtgaacgg ctcggtgctc aaggtcgtgc agaagagcga gagcccggtg gcgctcggcg
    11521 cgccgctgct ggaagtcgcc gacgcgagga gcctcgaagc cgtcgtggac gtgctgtcgc
    11581 aggatgcggt cgccgtgcgt cccggcatgg cggcgcgcgt cgagctcggc cagggcgtgg
    11641 cgccgctgcc cgccctggtg cgccgcatcg agccggccgc cttcaccaaa atctccgcgc
    11701 tcggcgtcga ggagcagcgc gtcaacgtga tcctcgactt cgccgggccg ctcgacacga
    11761 tctggacgct cggcgacgcg ttccgcgtcg aggcgcacat cgtcacctac cgcgccgagg
    11821 acgcggtcaa ggtgccggtg ggcgcgctgt tccgcgaggg cgagggctgg gcagtgttcg
    11881 tggcgcagga cggcgccgct gcgaagcgcg cggtcagggt ggcgcgcagg aacggcgccg
    11941 aggcgatggt cgaagacggg ctcgcggcgg gcgagcgcgt catcgtctac ccctcggacg
    12001 cgctgcgcga cggctcggcg atcgagattt cctccggccg gccgtgaccc cgacgcctga
    12061 cgacgccggc gccatgacct tggacctgga ggcggtggtg acgcgcacgg cgcgggtggt
    12121 gcgcgatccg ggcgatctgg agccagcggc gtgacgcggg cgacaccgca ggcgattctc
    12181 gaggcagcgg gcggccggcg cctcgccttg tttctcgatt acgacggcac gctcaccccg
    12241 atcgtcgagc ggcccgagga cgccgtgctc gccgaggaga cgcgcgcagt gctgcgcagg
    12301 ctcgcgcggc gccatgccgt cgccatcgtc agcgggcgcg atttgcagga cgtgcgcacg
    12361 cgcgtcggcg tcgaaggcct gcactacgcc gggagccacg gtttcgacat cgccggcccg
    12421 ggcggcggcc acgtgcacgg ggcagcgcgc ggcgccgagc cgcgcctcgc cgccgcggcc
    12481 gacgagctcg agacccgcac ggcgcagctc gcgggcgtgc aggtcgagcg caagcgcttc
    12541 gccgtcgcgg tgcactaccg gcgcgcgcgc aagtccgatg ccccgcgggt cgaggcggcg
    12601 gtcgaccgcg cgctggcgcg ccacccgggg ctgcgcaaga ccggcggcaa gatgattttc
    12661 gagctgcgcc ccgacgtcga ctgggacaag ggccgcgcgg tgcgctggct gatcgacgat
    12721 ctcgagctgc aggacgcagt gccggtctac atcggcgacg acctcaccga cgaggacgcg
    12781 ttccgcgcgc tcgccgggcg cggcgtgggt atcgcggtgc aggaatcgcc gcagccgacc
    12841 gcggccacct acacgctgcg cgatcccgac gaggtgcgcg cgctcctcgc gggcctggcg
    12901 tgagctggag cctcgactac cgcgacttca agcccgccga ggagcgcctg cgcgaggcgc
    12961 tgtgcaccct gggcaacggc tacttcgcca cgcgcggcgc cgcgcccgag gccgtcgcga
    13021 gcgaagtgca ctacccgggc acctacctcg ccggcggcta cgaccgccag ccgaccgaga
    13081 tcgccggccg ccgcatcgag aacgaggacc tggtcaacct gccgaactgg ctggcgctga
    13141 cctttcgcat cgacggcgcg gactggttcg atctgaaggc ggtcgaaatc ctcgaattcc
    13201 ggcagacgct cgatttctcc tgcggcgtgc tcgagcgcca cgtgcgcttt cgcgatgccg
    13261 cgggacggca cacgcggctc gacgagcggc gccttgtgca catggtcgag ccgcacctcg
    13321 cggccctgga gaccgtgctc accgccgagg actggagcgg gccgctggag ctgctcagcg
    13381 cgctcgacgc ccgcgtggag aacgccggcg tggcgcgcta ccgcgacctc gcgcgcaagc
    13441 acctcgagcc gctggaggcg cgcgccgcgg gcggggatat ggtgttcctc agggtgcgca
    13501 cgctgcagtc gcgcctcgag gtcgcgctcg ccgcgcgcac gcgcctggac cgcgccgccc
    13561 agacccgcgc gcgcatcgag cccggctacg ccggacagca cctgcacctc gagatgaaga
    13621 aaggcgagcc gctgcgcgcc gagaaggtcg tggcgctgca cacctcgcgc gaccgcgccg
    13681 tgtccgagtg cggcgaggcc gcgctcgagt cgtgtgcgcg cgcgcccggg ttcgccgcgc
    13741 ttgcggcggg ccacggcctc gcctggcgcc acctgtggca gcgcttcgac atcgcgctgg
    13801 aggacggcgg cgagcgcacg cagcgcatcc tgcggctgca cctgttccac ctgctgcaga
    13861 ccgcctcgcc caacagcaag atgctcgacg ccggcgtgcc ggcgcgcggg ctgcacggcg
    13921 aggcctaccg cggccacatc ttctgggacg agctgttcat ctttccgctg ctcaacttcc
    13981 ggctgcccga gatcacgcgc gcgctgctcc tgtaccgcta ccaccgcctc gccgcggcgc
    14041 gcgagaacgc ccgcgccgcg ggctaccgcg gcgccatgtt cccctggcag agcggctcga
    14101 gcgggcgcga ggaaagccag gtggtgcacc tcaacccgcg ctccgggcgc tggattcccg
    14161 accacaccta cctgcagcgc cacgtcaacg cggcaatcgc ctacaacgta tggcagtact
    14221 ggcaggtgac gcgcgaccgc gagttcctcg ccttctacgg cgccgagata ttcctcgaga
    14281 tcgcgcggtt ctggggcagc tacgccacgt tcaacgagac gcggcgccgc tacgagatcc
    14341 tcggcgtggt cggccccgac gagttccaca ccgcggaccc cgacgccggg cgaccgggcc
    14401 tcgacaacca cgcctacacc agcgtgatgg cggcctgggt gctggcgcgc gccttcgacg
    14461 tgctcgcggc gctgccggac gagcgccggc gcgagctgcg cgagacgctc gcgctgggcg
    14521 acgaggagct ggcgcgctgg gacgacgcca gccgccgcat gcacgtcgcc ttccacggcg
    14581 cgggcctgat cagccagttc gagggctacg agaagcttgc cgagctcgac tgggcgcgct
    14641 atcgggccga gtaccgcgac atctcgcgcc tggaccgcat cctcgaggcg gaagggcagg
    14701 acgtgaaccg ctaccagtgc tcgaagcagg ccgacgtgct gatgctgttc tacctgtttt
    14761 ccgcggagga gctggcggag ctcttcggtc gcctgggcta cccgttccca cccgacacca
    14821 tcccgaagaa cgtcgcctac tacgaggcgc gcaccacgca cggctcgacg ctctcgcgcg
    14881 tggtgaactc ctgggtgctg gcgcgctcgg accgcgccgg ctcctggcgc cagttcctcg
    14941 aggcgctgga gagcgacgtc ggcgacatcc agggcggcac gacgcccgag ggcatccacc
    15001 tcggtgccat ggcgggcacc gtagatctgg tgcagcgcgg ctacaccggc atcgaggcgc
    15061 gcggcgacgc gctctggctc aatccgcggc tgcccgagga gctcgcccgc ctgcggctca
    15121 ccgtgcgcta ccgcggcctc acgctggatc tcgaggtgcg ccaggacacg ctcgaagtgc
    15181 acgcacgcgc ctcgaacgtc gccccgatcg cgctcggcat cgtcgacgct gtgcaccgcg
    15241 tcgcggcggg cgaaaagcgc gccttcgcgc tgcgccgcga ggcgccgctc gcttgatcca
    15301 cgtcaatcgc gcccctcgcg cccggcgcac aatgaccctc atgccaccga tgtggatcgt
    15361 cgccgccgac agcagccagt gcaggatctt cgccgcgcac gcgcccgacg gtgcgctgca
    15421 ggagcttgag acactcagcc atcccgaagc ccggctgcag gagcaggcgc tggtgtccga
    15481 ctccccgggc cgcgctttcg acagcaaggg cgccgggcgc cacgcgatgg aagtcgaggt
    15541 cagcgcgaaa aagcacgagg cgatgatgtt cgccggacgc atcgccgctc gcctggatgc
    15601 gggccgcgtg cgccgggact tcgacaagct ggcgctggtc gcggcgcccg agtttctcgg
    15661 gctgctgcgc ggcaagctcg gcccgcagtt gcgcgcgctc gtcgtgcacg aggtcgacac
    15721 gaacccttcg gggatgaaag cggacgcgat ccgcgcgcgc ctgcccgcgc gcctgttccc
    15781 cgcgccgccc gtctaggacc gtcggcaggc ggcgtgcttg accttggtca aacgcgccgc
    15841 gcgtcgcgcc actacgctgg tagcgacaag gaggacccat ggcgccgaac cgcagggaaa
    15901 tgatcgagct ggcgcgcgcg atcgaggagc gccgcgaggc gctcgccacg gagatccggc
    15961 gcgaggtggc gcgtgcgcga aacgagccgt tcgccgaagt cgcggggacg gtgcacgaca
    16021 ccggcgacga ggcgctcgcc gacctggtgg ccgacgtgga caatgcggag gtcacgcgcg
    16081 acctcgccga gctgcgcgcg ctggagggcg cgcgtgcgcg catcgcgggc ggcagctacg
    16141 gcgtgtgcgc cgactgcggc gccgacatcc cggtggcgcg gctgcgcgcg cagctggcgg
    16201 cgctgcgctg catcgcctgc caggagcggc aagagaaggg ttacgctggc ggcgcccggg
    16261 cgaagctcta ggccgcactg cggcgtgcaa gcgccttgcg cagctccgcc agcggccgcg
    16321 cattgagcac gtccttcgtc tccagccacc cgcgccgcgc ctgccctact ccatagcgca
    16381 gcacgtcgaa gccgtcgacg tcgtgcgcgt cggagctgac cgcgaccagc acgccctcgt
    16441 ccttcgccat gcggcagtgc gtgtccgtga gatccaggcg gtccggatgc gcgttcagct
    16501 ccagcgccac tcccgacttc ttcgccgcgc gcacgagcgc gagcatgtcc acgtcgtagg
    16561 gcgcgcgctg ctcgatcagc cgccccgtgg ggtgcgccag gatcgaaaac agcgggttgc
    16621 gcattgcctt cacgatgcgc tcggtctgcc gcgcgcgcgg caggtcgaac ttcgaatgca
    16681 ccgcgcccac cacgagatcg agcttctcca gaatcgaatc cggcaggtcg agcgtgccgt
    16741 cctccaggat atccacttcg atgcccttca gcagcgtgat gccggagagc ttgccgttca
    16801 gctcctcgat ctctgcgatc tgcttcgcca ggcgctgcgg atccaggccg tgcgccaggg
    16861 tgagcttgcg cgagtgctcg gtgatcgcga gatacgaaag cccgtgcgcc ttcgcggcga
    16921 gcgccatctc gcgcagcgtg ttgtggccgt cggtggcccg cgtatgcgcg tgcaagtcgc
    16981 ccttcaggtt cttcagctcg agcagcgccg gcagctcgcc gcggcgcgcc gcctcgagtt
    17041 cgcccgcctc ctcgcgcagc tcgggctcga tgtaaggcag cccgaccgcc cggtacaccg
    17101 acaactcggt ctcgcccgcg acgcgcttgt ttgccttcca cacgccgtac tcgttgatct
    17161 tgagcccgcg cgcgatgccg agcttcctca cggcgatgtt gtgcgccttg gagccggtga
    17221 agtagtgcag cgccgcgccg aaggatgccg tcggcaccac gcgcaggtcg cactgaatcc
    17281 cgctcgagag cacggcgctc gcgcgcgtgc tgccctgcgc cagcaccttc ttcacctcgc
    17341 cgtaggtcac gaagcgcttc accgccggcg caggcttcgc ggcagcgacg aggaaatcga
    17401 ggtcgcccac cgtctccttc atgcgccggt agctgcccgc caccgtcgca ccgaggtatt
    17461 tcgcgaaggg ctcggcgtac tgcgcggcca ccgcgagctt gacgcgcggc ttcttcttcg
    17521 cgtgcgccgc ggcggcgtcc aagaggcgct gcgccgtctt ctcgccgaag cccggaatct
    17581 gcgcgagcct gcccgcgcgc gcggcgcgct cgagcgcttc cagcgaggag atgcgctgcg
    17641 cgtgcaacgc gcgcaggcgc ttgggaccga ggcccggtag cctcaggagc gcggtgatgc
    17701 cggcggggaa cttcttgcgc agccgctcga gcagcttgca gctgccgctt gccgcgatct
    17761 cgtggatctt gccgtcgagg tcggcgccga tgccggggag cttgggcagc tccctgcccg
    17821 cggcgagcgc cgcggcgagg tcgagcgaca ggccgcccac gatgcgcgcg gcgttgcggt
    17881 aggcgcgcac gcgaaacgga ttgtcgtcgc cgagctcgag caggtccgcg atctcctcga
    17941 acgcggccgc gatatcggcg ttatggaccg ccatggcccc gcgggccggt cagtggtcct
    18001 cgaggcgcgg gacgccgtcc tccgcgggct ggcccgtgtc cgggtcgagg taatccgaga
    18061 tgccgatctc ctcctcggcc tcctcgagcg tctccccgga ctcgagactg cgatcgccgc
    18121 cgagctccat gtcctgcacc gcctcgagca gcgtggggaa cgggccgtac tcgcggccgc
    18181 tctccttgtc ctgccagtac acgccgtcgg gccgctcgat gatgcgcgcg cggtcgtact
    18241 cgggcggggt ctcgggaatg ggctgagtca tgctgagcgt atcttacttt attgaggtca
    18301 atgcgccagc ccgacggcgt gaaaagctcg atccgggccg ccccgaaagg ccgggcgatg
    18361 cgcatcgcca tcgcgcgggc gaagccatgg gcgaaacggc ggcgtgccgc gcaaccgccg
    18421 tgacgcgccc taggaggtct ggcggaagcg cttgctcgcc tgacgcatgg cgagctccat
    18481 gtcgtgccag gccttctgca tcccggcgac gagcggcgcg ctcgccgcag cgctttgccg
    18541 accaagcttc gccagtgcgc ggttcgccgc cgcctgcttg cgcttgagca gctgcaggtc
    18601 gcggaccgcg gtcgcgcggg cgcttttctc cgctttgcgc gcttccgcca tcagccaatc
    18661 gagccgcttg cccaccgcgc gcacttcctt gtgcaggttg agccagcgct cgcccttgcg
    18721 cttggtcgct gccgtcctac gcttgcgtgc catgggggtt cctttcgtcg tctgccagaa
    18781 tccaatctag aggacacgcg gcattgcggt ttgacctgag tcaaaacccg ccatcgcgcg
    18841 tcaatgcatg ctttggacat caccccactg gggagagccc gcaatgcaac tgcccgtaca
    18901 gatcacctac catggcatgg aaagctctgc cgccctcgat aacgcggtgc gcgagaaggt
    18961 cgccaagctc gagcagtatc acccgcgcat cacaagctgc cgcgtcgtga tcgagcagcc
    19021 cgcgcagcgc aagcgtaagg gcaggcaatt cgtggtccgc gtcgacctga cggcgcctgg
    19081 caacgaaatc gccctgaacc acgaccacgc cgaggacgtg tatgtggcgc tgcgcgacgc
    19141 tttcgacgcg gcgcgccgca ggctcgaggt ccgcgcgcgc acgcggcgcg ccgagctcaa
    19201 gcggcacgag cgctagcgca tgaagcggct ttctcgcccc gccgcggacc gcaagatcgc
    19261 cggcgtgtgc gccggcctgg gcgaatactt cgacctcgac cccgtcttct tccggctgtt
    19321 cttcctgttc tcgatcctgt tcggcgggct cggtgccgtc gcctacatca tgctctggat
    19381 cctcgtgccc gcgggcgaag gcgcgcggcc ggcgcccggc gatggcaagc gcctgcggct
    19441 ctcgggcaag gagcgcatga tcgccggcgt gtgcggcggg ctgggagaat tcttcgagct
    19501 cgacccggtg ctgttccgcg ccgtgttcct ggtgctcgcg gtcatcggcg ggctggggat
    19561 cctgctctat ctcgtgctgt ggctcctcat cccggggccc gcgactgcag caggagcgcc
    19621 cccatcccca ggacgagcgc ggcaagcgcg atcagcacac cgagccaggg aataagcgcg
    19681 gcgacgccga ggaccaggac gccgagcgcg gcgaacagcg cacgccagcc gcggtgggcg
    19741 gcgcgcgccg cccaccagcg tgcgagcgcc gcgtcgccca gcgcgatgcc ggcggcgaca
    19801 tagcccagca gcagcagcgc cagatagccc aggacgccgg ccagcgcgag cggaataccg
    19861 accaccgtgg cgagcaggat caccaccgcg aggggcaccg ccgccagcgc gagaaatccg
    19921 gcgagcaggc tccagcccag gcgcgcccgc gccgcggcgg agacgcggct ggagagtccc
    19981 ggcagcgcga gcaccagcac cgccgccatc accatcagcc cgagcgtcca caccccgaac
    20041 acggccggcg caggcgaacg ctcggcgacg cggcgcacgg gcatctccag gcgctcgatg
    20101 ccgccctcga cccgcgccgc cgcgtcctgc cggagcggcg cgccgccgtg gtagcgcagc
    20161 ctgccgccga tgcgcgcgtt cgggccgagc tcgagctcgc ccgcgaccgc ttcgacgtcg
    20221 ccgcccacgc gcccgtcgat gagcacgcgc ccgcccgcag cctgcaggta gccgccgacc
    20281 gcgccgagca tctcgacccg gccgccagcg aggctggcgt tgcccgagat gcgcgcctgc
    20341 ggcccgacgc tcaggttgcc tcccgcgacg cgcgcgttgc gcgcgagcgt gccttcaacg
    20401 gcgatattgc cgcccgcggc atacaggttc tggctgccgg atccgtccag gcgcagcgcg
    20461 ccgccgacgg ccacgatgtc gcccgccacc ggggcggcga ggcgcagctc gccgcccgcg
    20521 gcgagcagat cgccggcgac cggctggctg acgcgcgcgc tgtcgcccgc gacgaacgta
    20581 tcggcgccct gctggcgcag gtacaggtgc tcgccgcgct cgtcggccgt cgcgcccgcc
    20641 aaaggcgcgc acagtagcaa aaggctcatc gcacgaacgg cgatcatgcg tacccttctt
    20701 cagccaggtg tgcgaatgcg cgcggcgcgc ttgcgcttac acttcatcgc gccgcacgcc
    20761 cttgcggacc cacacgccgc gcggcgcgca ttccagcacg tcgctgccgc tgcgcaccgt
    20821 gtcgccgatc gcatagacct ggccgttgaa gtagcaggca ccggcttcca gctcgacgtc
    20881 gaacatggca tcccgatcgt cgtactcgga gtggtcgacg atgggcgagt tcttcgcctc
    20941 cggatccggc gcaccgacct gcggcatcgg gtctttcacg ggggcatctc cggatggtta
    21001 cgctgctcag gctgtcacca atcccgcacc cgcgcttgat ctcagtcaag ccaggcgcgc
    21061 ttttcgagcc acgccgcgat tgccgtggcg ctctccgcgg gcgtgccgcg gtcgccgtgc
    21121 accacgagct ccggcgccgc gggcggctcg taggcggcct gcgcgcccgg cagcgtggcg
    21181 cttcggccct cgcgcgcggc gcggtacagg cccttcgtat cgcgcgcggc gcacacctcg
    21241 agcggcgtgt cgacaaacac ttcggcaaag cgtgcgatct tcgcgcgtgc tgcgtcgcgg
    21301 tagacgcgca ggttggcggt cgcgtcgaac agcaccgggc ggccgcgccg cgcgacgtcg
    21361 cttccgagct gcacgagcgc tgcgtagaag aagtcgcgct cttggggcgc ataagtcgca
    21421 aacggtgtga gctgcgtgcg caagacgtcg gactcgagca ccgccgcctc gacgccgcgc
    21481 gcgtgcagct gcgccaggag ctcgcgcgcg atcgcgctct tgccggagcc cgaaaggccc
    21541 gtgagccaca cggcgaagct catgcgccgc agtaggcgtt ggcgcgcgcc gggtcgaagc
    21601 gcggcgccgc gagcacgttg agcgcgaaat cgagcatctt gcgccgcaac cgcgcatcga
    21661 gcgcggggta ccacagcgga ttcgccatca ccagcgcgcg aaaagcgaag aaaggcgccg
    21721 cgacttcgag catctccagg tcgccggagc cctcgagata gcgtccccag aaaccgcgcc
    21781 acagacgctc gaaatcgccc tccagccggc ccgagcgctg cagcgagaag aacaggtagt
    21841 tgagcgcgag gcacgtgacg tcgtcggccg gctcgcccca ctcgccgcgc gagcggtcga
    21901 gcaccgtgaa gtcggtgcct tcgcggaaca gcaggttcca ggggtgaaag tcgccgtgca
    21961 cctggcgcag gcggtgcgcc cgcggcttga gccgccagcg ccattccagg cagcggcgct
    22021 cgatctcgcg cacgctctcg aggtacgggc cctcggcgta gctgtcggcg acgccgaaaa
    22081 tgcactcgcc gtgcccgagc aactcgcgca cgcgtcgcac atacagcccc ggatcgggac
    22141 cggctctgcg gtggatctcg atgaggtagt cgcacagcgc gtcggcgcgc gcgaaatcct
    22201 gcggcagcgc gtccgcgccg ccgcgcaggc gcagcaggtc atcggcgtac tcgtggcctt
    22261 cgacgaactg cgccagcatg aacagctcct cggccgagcc gagcggctcg agcgcgccgc
    22321 cggcgcgcac cgcgccgaca tcgagcgaag gcacgtgccg gggcaggcgg ttgaaggcgc
    22381 cgtgcgccca gagcaggagc tgcgcgcgat cggccatgtg ctcgtgtccg aacggacccg
    22441 ggcgcacggt ttcgagcacg gcgcgctgcg cctggccggc gcgctcgtag tccacgcgca
    22501 gcggcacgcc gtagccgtag cccttgtcgc cctggcggcc gccgccgagc ggcgtcaggc
    22561 gcacgttcgt tacctcctcg ccgtgcagcg cgcgcaggta atcgcgcagc gccgccggcg
    22621 taaacgggct ggcgctcatg cagccattgt gcggccgcgg cgcagcgcgg cgttgacctc
    22681 gatcaaacag cgcatcgagt aggatgcggg cctatggaaa agctcaccgg ccgcaacgcg
    22741 ttcctgcgcc ttctcgtcga ggaaggcgtc acccacctct tcggcaaccc cggcaccacc
    22801 gagctggcga tcatggaagc ggtgcccgat tttccgcagc tgcgctacgt cctcgggctg
    22861 cacgagtcgg tggtggtggc gatggccgac ggctatgcgc gcgcctccaa ccgcctcgcc
    22921 gcggcgaacg tgcacgtcgc gccgggcctg ggcaacgcca tgggcgcgct ctacaacgcg
    22981 cagttctcgg gctcgccgat catcctcacc gcggggcagc aggagcaggg ccatggcctt
    23041 ggcgagccgc tgctctatgc gccgctcgtg ccggtcgcca cgcccttcgt caagtgggcg
    23101 gtggaggcga cgcgggtcga ggacctgccg cgcatcatcc ggcgcgcggc caaggtcgcg
    23161 ctcacgccgc ccaccgggcc ggtattcatc tcgctgcccg gcgacgtgct cgagcagcag
    23221 tcggaaatcg agctcggcgc gccgacgcgc atcgacgcgc gcacgcggcc gtccgacgag
    23281 gccctggccg agctcgcgcg gcgcctcctt gacgcgaaga atccggtgat cgtggcgggg
    23341 cacgagctct cgatccacga cgctttcgcc gaggcgaccg agctcgcgga gctcctcggt
    23401 gctgccgtgt accagcagac ggtgccctac gcggcgcagt acccgaccgg gcaccgcgcc
    23461 tatctcggcg cgctcacgcg cgtgcagaaa tcggtgcgcg ccgcgctcga gccgttcgac
    23521 ctgctgcttt gcctcggcgc ggacctgctg cgcatgtccg tgtacagccc gctcgatccg
    23581 ctgcccgcgg gcttgccggt gatccacgtg agcgagcgcg actgggagat gggcaagaac
    23641 catcccactg cgatggcgct gcatgccaac gtgaaggaga cgctgcgcgc gctgctgccg
    23701 ctcctgcgca cgcggcgcgg cgccgaaggc gccgccgccg ccgagcggcg ccttgccgcg
    23761 ctcgcgccgc gcaactggcg cgcgcagcgc gacaaggcgc gcctcgccgc gctcgaggcg
    23821 gccgaggaaa agccggtcga cccgcgcttc ctgatgctgc gcttcgccga ggcgctgccg
    23881 gccgacgcgg tggtggtgga agaggcgctc acctcgagcc tcagcctgcc cgcggtgctc
    23941 gagcagcgcg acgcgaagag cttctacggc ctggcgagcg gcggcctggg atttgcgctg
    24001 ccgggcgcga
//
LOCUS       DBNHKE010000028         5931 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_56978, whole genome shotgun sequence.
ACCESSION   DBNHKE010000028 DBNHKE010000000
VERSION     DBNHKE010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_56978"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="xseA"
                     /locus_tag="AC16O9_01600"
     CDS             <1..743
                     /gene="xseA"
                     /locus_tag="AC16O9_01600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6515346.1"
                     /translation="DVLTTLRRRNPSIPVIVYPVPVQGDGAAAKIAAMLAAASARAEC
                     DVLLLVRGGGSIEDLWQFNEEIVARAIRASSIPVVVGVGHETDVTIADFAADRRAPTP
                     TAAAEMVSPSREELLVRVAELAQRATREALRRIEYAMQTVDALSRRLVHPRERLRTSR
                     QLLEQLAARLSSAAAHRLDNLAAQLAALRAGLVSLNPSAVLERGYSLTRNAAGEVVAD
                     ASRVAEGDRLTTTLAKGWIESEVTKKGR"
     gene            882..1058
                     /locus_tag="AC16O9_01605"
     CDS             882..1058
                     /locus_tag="AC16O9_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515347.1"
                     /translation="MPARSPSKLGLTLTFCLLAGLLVWRFAVPRASPWLDVAALAAVA
                     VLLLALRTFLKKKS"
     gene            1055..1576
                     /locus_tag="AC16O9_01610"
     CDS             1055..1576
                     /locus_tag="AC16O9_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515348.1"
                     /translation="MTTWSLLLLLTLPAAGADQTDDPMIRWDCDRAGNQLTVQMVRPP
                     VENVNRREVLLLSGAQNFEQCHINGASWTLLVDIIEYDSGPCEPFPDTIVSLLRNDKL
                     VVSKTHVGQNCFDRPVLSALRVTEPVDGVNPALEVCAAPAYGTGNSCFPLKLRDMGNA
                     IDNDLIASRAKKR"
     gene            complement(1573..2418)
                     /locus_tag="AC16O9_01615"
     CDS             complement(1573..2418)
                     /locus_tag="AC16O9_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6515349.1"
                     /translation="MTNNLRGVLWMGGAVLSFSTMAVAARELLAHMGPFQILFVRSAV
                     MLAIVLVIVAQAENAVTATRRLPLHVFRNVIHLGGQYCWVFAIGALPLATVFAIEFTM
                     PMWTAILAFIFLGERLSRPRVVMLVLGLTGVLIILRPGFGFFHLGALVALAASLFYAG
                     NMIATKQLSGTDSPLAVLFWMSVVQTPLALAAALPGWVSPPISHLPWMVLIGVGSYTA
                     HYCMTRAFKLADATVAVPIDFVRLPLIAVVGTLFYDEPFDPMIIVGAAVIFTGTYYSL
                     SRERR"
     gene            complement(2431..3735)
                     /locus_tag="AC16O9_01620"
     CDS             complement(2431..3735)
                     /locus_tag="AC16O9_01620"
                     /inference="COORDINATES: protein motif:HMM:NF017135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HIR6515350.1"
                     /translation="MNQDATTKAPGAFDRLRGWGQRLAAAVRSGLAPGQAPRTAAGLA
                     GELERLLAESRALRGGERSARERAQRIAALYRGADAEERAAILGHIARAFMPERAALD
                     QAVAAMREAADDIARGHAEARLRIALDAPRARFFAQFNLLPEGVKFLVDLRADLLAFL
                     EHEPALDVLRVELDGLLESWFDPGFLQLTRITWQSPALVLERLIAYEAVHRIESWEDL
                     KNRLDRDRRCYAFFHPRMPNEPLIFVEIALAKGLPGSVQELLDAQAPIGEVRDADTAV
                     FYSISNAQKGLRGISLGNLLLKRVIAALQRDLPWLKTFCTLSPLPGFRRWLERAAPAL
                     AGDGAPLQALREPLLKACARYLVQEKSNGLPLDPVAQFHLHNGASIDRIFWAADASAR
                     GQAQSRGLMVSYRYEPAEVDHNHEEFLKSGHIAAAKRVLRLL"
     gene            3801..4184
                     /locus_tag="AC16O9_01625"
     CDS             3801..4184
                     /locus_tag="AC16O9_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515351.1"
                     /translation="MKAMACAALCALLVVRGALGNVEPYLAASHGTKCDLETDGALTC
                     RYRIGASLEFTLQRVGEPGMRLEVLRNEREGDYALDPEPRSTCLVVRHGWSARPAAGT
                     ERNTAAISTVNGIAYRTLRECRKAK"
     gene            4504..5343
                     /locus_tag="AC16O9_01630"
     CDS             4504..5343
                     /locus_tag="AC16O9_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HIR6515352.1"
                     /translation="MAPSTAVIRALRQVCTPLARLMLAKGITYPQFAELMKGVFVEVA
                     EREFRLVGKAQTDSRVSLLSGVHRKDVRRLRSAPPDADSVAAKSVSLGAQLVAAWTGL
                     RRFQDKQGRPLPLPRLHSQGAGVSFESLAERVSTDIRARAVLDELLRLGVVHLDEQDR
                     AVLDAQAFVPRGGEEEKLVYFGRNVRDHAAAAAHNVLGAGEPFLERSVHYDALSAAST
                     RELAALAETQGMSAVQSLNRRAIELEERDAATPGARRRFTFGVYFYDADAGDEAPRAP
                     AHV"
     gene            5336..>5931
                     /locus_tag="AC16O9_01635"
     CDS             5336..>5931
                     /locus_tag="AC16O9_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515353.1"
                     /translation="MCSAASRTLRALLAAAALAFAGSAAQAQGGLNLSLGADYAQGKY
                     GTAVTSKQWTVPLVAKHEADAWSAKLTLPYVRIENPAIGRDGTPLPCAGSATAPRTAS
                     GLGDLVLTLSRNVFEDRAERMLIDLTGKVKFATADEAKCLGTGEDDFYVQGDLSKGYG
                     SIGIFGTLGWRKMGDPPGVAFEDAFYFSIGGSYRVTAER"
BASE COUNT          946 a         2081 c         2062 g          842 t
ORIGIN      
        1 gcgacgtgct aacgactctg cggcgccgca atccgtcgat cccggtcatc gtctacccgg
       61 taccggtgca gggcgacggt gcggccgcga agatcgcggc gatgctggca gcggccagcg
      121 cgcgcgccga gtgcgacgtg ctgctgctgg tgcgcggcgg cggctcgatc gaggacctgt
      181 ggcagttcaa cgaggaaatc gtggcgcgcg cgattcgcgc ttcttcgatt cctgttgtcg
      241 tcggcgtcgg ccacgaaacc gacgtcacca tcgccgactt cgccgccgac cggcgcgcgc
      301 cgacgcccac tgcggcggcg gagatggtga gcccgtcgcg ggaggaactg ctcgtgcgcg
      361 tggccgagct cgcacagcgc gcaacgcgcg aagcgctgcg gcgaatcgag tacgcgatgc
      421 agacggtgga cgccctgtcg cggcgcctcg tgcatccgcg cgagcggctg cgaacctccc
      481 ggcagctgct ggagcagttg gctgcgcgcc tgtcgtcggc ggcagcgcac cggctcgata
      541 acttggctgc gcagctcgca gcgttgagag ccggtctcgt gagcctgaat cccagcgccg
      601 tgctcgagcg cggctacagc ctgacgcgca atgcggcagg cgaggtggtg gcggatgcct
      661 cgagggttgc cgagggcgat cggctgacga cgacgctggc gaaggggtgg atcgagagcg
      721 aggtgacgaa gaaaggccgc taggtcctgt cctctgtatt catgacgcct gcagccagac
      781 gcgcgccgtt tacgtggttt gtggatgcgg ggatggactt tcgcacgatc ccggttcgcg
      841 acgaccgcgc gattgcgcgt gccgtagtac cctttcggga aatgcccgcg cgcagcccgt
      901 ccaagctcgg actcaccctc accttctgcc tgctcgccgg actcctcgtc tggcgattcg
      961 cggtcccccg cgcctcgccc tggctcgacg tcgcggcgct cgccgcggtc gcggtgctcc
     1021 tcctcgccct caggacattc cttaagaaga aatcatgacg acctggagcc tccttctcct
     1081 ccttaccctc cccgccgccg gcgccgacca gaccgacgac ccgatgatcc gctgggactg
     1141 cgatcgggcc ggcaaccagc tcacggtgca aatggtccgc ccgccggtgg agaacgtaaa
     1201 ccgccgggag gtcctgctgt tgagcggcgc gcagaacttc gagcaatgcc acatcaacgg
     1261 cgcgagctgg acgctgctcg tcgacatcat cgagtacgac tccggcccct gcgagccgtt
     1321 tcccgacacc atcgtcagcc tgctccgcaa cgacaagctg gtggtctcga agacgcacgt
     1381 cggccaaaac tgcttcgacc ggccggtgct gagcgcgctg cgcgtgacgg agccggtcga
     1441 cggcgtgaac ccggcgctcg aggtctgcgc agcgcccgcc tacggcaccg gcaacagctg
     1501 tttccccctc aagctgaggg acatgggaaa cgcgatcgac aacgacctga tcgccagccg
     1561 cgcgaagaag cgctagcgcc gctcccggct caggctgtag tacgtcccgg tgaagatcac
     1621 cgccgccccg acgatgatca tggggtcgaa cggctcgtcg tagaacagcg tccccaccac
     1681 cgcgatcagc ggcaggcgca cgaaatcgat cggcaccgcc acggtggcat cggccagctt
     1741 gaacgcccgc gtcatgcagt aatgcgcggt gtagctcccc accccgatca ggaccatcca
     1801 cggcaggtgc gagatcggcg gcgataccca gccgggcagc gccgccgcca gggcgagggg
     1861 cgtctgcacc accgacatcc agaacagcac cgccaggggc gaatcggtac cggagagctg
     1921 cttggtggcg atcatgttgc ccgcatagaa caacgacgcg gccagtgcca ccagcgcccc
     1981 caggtggaag aatccgaacc ctggccgcag gatgatgagc acgcccgtca gcccaagcac
     2041 cagcatgacc acccgcggcc gcgacagccg ctcccccagg aagatgaacg ccagaatcgc
     2101 ggtccacatc ggcatggtga actcgatcgc gaacaccgtc gccaggggca atgccccgat
     2161 ggcgaacacc cagcagtact gcccgccgag gtggatcacg ttgcggaaca cgtgcagcgg
     2221 caggcgccgc gtcgccgtca ccgcgttctc ggcctgcgcg acgatcacca gcacgatggc
     2281 aagcatcacc gcgctccgca cgaacaggat ctggaacggc cccatgtggg cgagcagctc
     2341 gcgcgccgcc actgccatgg ttgaaaagga gagcaccgcg ccccccatcc acaggacgcc
     2401 gcgcaggttg ttcgtcatcg gcggcgccga ctatagcagg cgcagcaccc gcttcgcggc
     2461 ggcgatatgc ccgctcttga gaaactcctc gtggttatgg tcgacctcgg cgggctcgta
     2521 gcggtagctc accatcaggc cgcgcgactg cgcctggccc cgcgcggacg cgtccgccgc
     2581 ccagaagatg cgatcgatgc tcgcgccatt gtggagatga aactgcgcca ccggatcgag
     2641 cggcagcccg ttcgacttct cctgcacgag gtagcgcgca caggccttga ggagcggctc
     2701 gcgcagcgcc tgcagcgggg cgccgtcgcc cgcgagcgcc ggcgccgcgc gctcgagcca
     2761 gcgccgaaag cccggtagcg gcgacagcgt gcagaaagtc ttcagccacg gcaggtcgcg
     2821 ctgcagggcg gcgatgacgc gcttcaagag caggtttccc aggctgatgc cgcgcagccc
     2881 cttctgcgcg ttggagatcg agtagaacac cgccgtatcc gcgtcgcgca cctcgccgat
     2941 cggcgcctgc gcatcgagca gctcctgcac gctcccgggc aaccccttgg cgagcgcgat
     3001 ctcgacgaag atcagcggct cgttcggcat gcgcgggtgg aagaacgcgt agcagcgccg
     3061 gtcgcggtcg agccggttct tcaggtcctc ccaggactcg atgcggtgca ccgcctcgta
     3121 ggcgatcagc ctctccagca ccagcgccgg agactgccag gtgatgcgcg tcagctggag
     3181 aaagcccgga tcgaaccagc tctcaagcag cccgtcgagc tcgacgcgca gaacgtcgag
     3241 cgccggttcg tgctccagga acgcgagcag gtcggcgcgc aggtcgacga ggaacttcac
     3301 gccctcgggc agcaggttga actgggcgaa gaatctggcg cgcggcgcat cgagcgcgat
     3361 gcgcagtcgc gcctcggcat ggccgcgcgc gatgtcgtcg gcggcctcgc gcatcgcggc
     3421 caccgcctgg tcgagcgccg cgcgctcggg catgaaggcg cgggcgatgt ggccgaggat
     3481 cgccgcgcgc tcctcggcgt cggcgccgcg gtacagcgcc gcgatgcgtt gcgcgcgctc
     3541 gcgcgcggag cgctcgccgc cgcgcaatgc gcgcgactcg gcgagcagcc gctccagttc
     3601 gccggcgagg cccgcggccg tgcgcggcgc ctgtcccggc gcgagccccg agcgcaccgc
     3661 ggcggcgagg cgctggcccc agccgcgcag gcggtcgaac gcgccgggag ccttggtggt
     3721 cgcgtcctgg ttcacggtcg gaacggttgc aggtttctgc gccggtggca agtccggtgc
     3781 gcgcgcctac aatcggccgc atgaaggcga tggcgtgcgc ggcgctctgc gccctgcttg
     3841 ttgtccgcgg cgcgctgggg aacgtggagc cgtacctcgc cgcatcgcac ggcaccaagt
     3901 gcgacctcga aaccgacggc gcgctcacct gccgctaccg catcggcgcg agcctggagt
     3961 tcacgctgca gcgggtcggc gagcccggca tgcggctcga ggtcctgcgc aacgagcgcg
     4021 agggcgacta cgcgctcgac cccgagccgc gcagcacctg ccttgtcgtg cgccacggct
     4081 ggtcggcgcg gccggcggcg ggcaccgagc gcaacaccgc ggcgatctct actgtgaacg
     4141 gcatcgccta ccgcacgctg cgcgaatgcc gcaaggcgaa atagggggac aggcacgccg
     4201 cgcgccgggt gggggcccgg cgcgcggcgg cggacggact actggacggt ttgggggggg
     4261 atcgcccgtc agtgtcgccc gctgttgcct gattcggacc gctcgatgcg ctcggacgga
     4321 ccggatcggt ccggacgatc cgcgtggcgg gcgccccgca gcgcccccgg cggggtccaa
     4381 acgcacccgc agagtacgtg ggaaaaattc ccatgtcaac tattgtgtgt ggaacctttt
     4441 ccctcatgcc ctgcattgca atatagtggc gccctgaacc gttcacgccg acagggaggg
     4501 cgcatggctc cgtccacagc cgtaatccgc gcgttgcggc aggtctgcac cccgctcgcg
     4561 cggctgatgc tggccaaggg catcacctac ccgcaatttg ccgagctgat gaagggcgtg
     4621 ttcgtggagg ttgccgaacg ggagttccgc ctcgtcggca aggcgcagac cgacagccgc
     4681 gtcagcctgc tctcgggcgt gcaccgcaaa gacgtgcgac gcctgcgtag cgcgccgccg
     4741 gacgccgaca gcgtcgccgc caagtcggtg tcgctgggcg cgcagctggt cgcggcgtgg
     4801 accgggctgc gcaggttcca ggacaagcag ggccggcccc tgccgctgcc ccgcctgcac
     4861 agccagggcg cgggggtgtc gttcgagagc ctggcggagc gcgtcagcac cgacatccgc
     4921 gcgcgcgcag tgctcgacga gctgctgcgc ctgggcgtgg tgcacctgga cgagcaggat
     4981 cgcgccgtgc tcgacgcgca ggccttcgtg ccgcgcggcg gcgaggaaga aaagctggtc
     5041 tatttcggac gcaacgtgcg cgaccacgcg gcggcggccg cgcacaacgt gctcggcgcg
     5101 ggcgagccct ttctggagcg cagcgtgcac tacgacgcac tgtcggcggc ctccacccgc
     5161 gagctcgcgg cgctcgccga gacgcagggc atgagcgccg tgcagagcct caaccggcgc
     5221 gcaatcgagc tggaagagcg cgacgccgct acgccgggcg cgcggcgccg gttcaccttc
     5281 ggtgtgtatt tctacgacgc ggacgccggt gacgaggcgc cgcgggcgcc ggcgcatgtg
     5341 tagcgccgcc tcgcgcactt tgcgcgcgct gctcgcggcg gccgcgctcg ccttcgccgg
     5401 atcggcggcg caggcgcagg gcggactgaa cctgtcgctg ggcgccgact acgcgcaggg
     5461 caagtacggc acggcagtaa cctcgaagca gtggaccgtg ccgttggtgg cgaaacacga
     5521 ggcggacgca tggagcgcca agctcaccct gccctacgtg cgaatcgaga accccgccat
     5581 cggccgcgac ggcacgccgc tgccctgcgc cggctccgcc acggccccgc gcaccgcgag
     5641 cgggctcggc gacctcgtct tgacgctgtc gcgcaacgtc ttcgaagacc gcgctgagcg
     5701 catgctgatc gacctgaccg gaaaggtgaa gttcgccacc gccgatgaag cgaagtgcct
     5761 gggcacaggc gaggacgatt tctacgtgca gggcgacctc agcaaaggct acggctcgat
     5821 cgggatcttc ggcacgctcg gctggcgcaa gatgggcgac ccgccgggcg ttgccttcga
     5881 ggatgcgttt tacttctcga tcggcggctc ctatcgcgtg accgccgagc g
//
LOCUS       DBNHKE010000029        23901 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1432359, whole genome shotgun sequence.
ACCESSION   DBNHKE010000029 DBNHKE010000000
VERSION     DBNHKE010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 23901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23901
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1432359"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..536)
                     /locus_tag="AC16O9_01640"
     CDS             complement(117..536)
                     /locus_tag="AC16O9_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6515354.1"
                     /translation="MTYDRAAQFLARLDQLRPMQRFLEQFCAESNLPRAAYLRLNLVL
                     EELFTNTVKHGHRGDCDAPVWVAVSRLGDTLSVVYEDKAPPFNPYAHLTEAVVDTTVS
                     MRKIGGLGVLLTRELAASRDYAYLFGRNRIRLTLAAA"
     gene            595..1038
                     /locus_tag="AC16O9_01645"
     CDS             595..1038
                     /locus_tag="AC16O9_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6515355.1"
                     /translation="MRHFLAVAVLALAVTGFARAQDVGHVKVAKGTVHVERDGKRLPA
                     AVGMTIRQSDTVLTGADGSAGVTFSDNSLLSTGPNSVLVVDHYAFDSTTHAGKFDASL
                     KKGTLAVVSGKIVKQSPGAMRVRTPAAIMGVRGTEFMVQVDEPAK"
     gene            1076..1639
                     /locus_tag="AC16O9_01650"
     CDS             1076..1639
                     /locus_tag="AC16O9_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6515356.1"
                     /translation="MVFLAPDRVGNELVVVVPSADGHVGVVIVERGDKKLTLDTAFAA
                     SRIVDGGAPAAERFGGADAVRAEFADVLAALPDRPQSFLVYFLEGTDEFTEESGIEFT
                     KILSELRERSAPDVVVIGHTDRVGNVQYNDDLSLQRAERVRGELVRLGIPPARIRVAG
                     RGEREPLVATDDDVAEPRNRRVEINVR"
     gene            complement(1574..3706)
                     /locus_tag="AC16O9_01655"
     CDS             complement(1574..3706)
                     /locus_tag="AC16O9_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR6515357.1"
                     /translation="MSSAWFKRAGPLALVIALLLVLASEPPFVFGLRHALFDGYQRLF
                     PRERISAPAVIVAIDEQALERHGQWPWPRTRVAQLLAGISDAGPAAIGVDLLFAEPDR
                     FSPAEVAREVQELSVDVARRLRALPSNDAALAAALKARAVVLGIAGLEAPDPRFERPP
                     AAAPIRITDGADFALRRFAGHLQSLPEIDRAAAGRGLLSVDPEGNVIRRALAAARIGD
                     VIVPALSVEMLRVATGAPLALIARGRDQVEVRLGDIAIPAHADGRFWIRYGPHDPARF
                     VSAAQVLEGSVAAPELQDKLVLVGVTGLGLLDYQTTALGERIPGAEIHAQILEQIFDG
                     AFLRRPSTLHALELGLLAVAALMFALTVPSLGVATSIALYAVVIALLTALGIEGFLLE
                     GLLLDVAWPAIGATVVFGIVLAQALSEADEQRRRLREQAARVSGELESARRIQMGLLP
                     APRALFAHEQRFAIDALLEPARTVGGDFYDCFMVDSERLFFIVADVSGKGLPASLFMA
                     LSKTLLKSAARRGGDVGGLMVRANAEVSSENPESLFVTAFAGLLDARTGMLEFCNAGH
                     EPPFARAPGGALERLVHAGGPPLCVMENYAYPIEHRALAAGEWICLVTDGVTEAMNAA
                     GELYGAARLRSVLEALPAGVGPSELLAAVRADVGRFVGAAEASDDLTLLCVRWNGTAA
                     LPAGPDDSADVDLDAPVPGLGDVVVGRH"
     gene            complement(3703..4659)
                     /locus_tag="AC16O9_01660"
     CDS             complement(3703..4659)
                     /locus_tag="AC16O9_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6515358.1"
                     /translation="MRVRFWGTRGSIPVALTSADIRDKVALALFKAEGRRFESVGEAH
                     DFALKHLDFSLTHTFGGHSPCVELETGAQEYLVCDLGSGARPFGAHVMARQSGKPATV
                     NVFMSHVHWDHIMGFPFFGPAYVPGTHIRIHGCHDVIEQAFRLQQAPPCFPVEFSQLA
                     ADIEFVKLAPGRPHEFSGIRVTPTPQMHSGESFGYRFEVGGKVVVYTTDSEHKLENRA
                     QTERFVRFFQGADLVVFDAMYSLADAISVKADWGHSSNIIGVELCQMARAKHLVLFHH
                     EPAYDDATIEGVLRETRRFEELTRGTQPLRISAAYDGLEIDV"
     gene            complement(4665..5039)
                     /locus_tag="AC16O9_01665"
     CDS             complement(4665..5039)
                     /locus_tag="AC16O9_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6515359.1"
                     /translation="MPLAHRLYDNALVLTPTGRLDHDNCEAFREELAPHLERSAKEGM
                     SLVLDLSGLEYVSSAGLRCFMLAAKQAKAQNSRIVIAAMQPVVAEIFQIARFNLVFEA
                     FPKVRDALTALSPAAAAAFDRG"
     gene            5106..5912
                     /locus_tag="AC16O9_01670"
     CDS             5106..5912
                     /locus_tag="AC16O9_01670"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR6515360.1"
                     /translation="MRADDASRVHRAARWLVRQHQGRLASRPMSRGLTPRTADEAYAV
                     QDAFVRMKARYCGRVTGHKIALTTPRMRAMVGLETPIGGQLHQRQVVRGPASVQAAQY
                     GRLIVEFEIGMQMAHDLPPERAPVTMGQAADAVGAVMPALELADDRNADYATLSARGL
                     ELAADNAWNEGAVLGAPVDDWRDVDLAGLRGSALINGAVVGEGRGADSMGHPLAALAW
                     VANHLAGRGQGLRRGDVVITGSLVTSKFPRSGDRVRFEAGALGAVELQVA"
     gene            complement(5909..7318)
                     /locus_tag="AC16O9_01675"
     CDS             complement(5909..7318)
                     /locus_tag="AC16O9_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6515361.1"
                     /translation="MDLSRIVEQWARHAPSRIALRFEGEQWTYGELWQRIEHVARALE
                     VAKGDRIAWLGYNRPDMLVLLFAAARRGALLVPLNWRLTAAEHRGILDDSCPRWMFAD
                     ADFAEAARALGVPMGQLDAPAGTASAGADEDDLLIVYTSGTTGRPKGAVLAQSALLWN
                     GYNAIHAHKLGQADHVLSTLPMFHVGGLNNQTVPALLAGATVTLHKRFEPGRWLREVH
                     AARPSLSLLVPATMQAVIAHPDWARTDLSSLRLLMTGSMVVAESLIHAFHQRGVPVGQ
                     IYGSTETAPIALVLTREDALRKVGSTGKPACHCEIKLVHGEICVRGPNVMRRYWNDPV
                     ATARVLDAEGWFHSGDLARVDDEGHYWIMGRSKDVIISGGENIYPAELESVLADCPAI
                     AESTVIGVQDDKWGEAACAVVVRRAGATLTEADVLGLFKDRLARYKHPRQIVFADALP
                     KNALGKVQKNELRKLLQSR"
     gene            complement(7320..8573)
                     /locus_tag="AC16O9_01680"
     CDS             complement(7320..8573)
                     /locus_tag="AC16O9_01680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6515362.1"
                     /translation="MHDRVPQSDPRYAVAGSNYPLSDKQKALLAQAEALGPRFAGRAE
                     RYDRDASFPVENYADLRDAGMLGLCVPEKYGGKGADLKTYALVSATLGKYCGATALTF
                     NMHACSTLWPGVLADDLEMTEAQRAEHERYRALHFERVVRKGAVYAQPFSEGSAAAAG
                     KAPFGTTADKVDGGWLVNGKKIFASLSGAADYYGVLCTENKPELSMRDTMYLAVPGDA
                     PGFSIVGDWDPLGMRGTVSRTLVFKDVFVPDDGAMMPRGVYFQAASRWPHMFTTLSPT
                     YMGIAIAAYEFTVRYLRGEVDGMPPVKRRMYPTKQIAVAELWVRLEQTRALFLRMLED
                     ARVDPPKDARLRAYAAQYTIMENANDICRLAIRTCGGQSMLKSLPLERLYRDSRCGAL
                     MLPWTAELCLDRIGREALYESGEVD"
     gene            complement(8582..9316)
                     /locus_tag="AC16O9_01685"
     CDS             complement(8582..9316)
                     /locus_tag="AC16O9_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6515363.1"
                     /translation="MSPLLFLHGVGGGSAAWARQLPYFAARGYRARAWDQPGYGGTAP
                     VEPYDLAQVTAALERSIGDEPAVLVGHSMGGFIAQEAYTRFPDRIRALGLCFTSAAFG
                     GGSGEFARQFIAARIAPLDAGKSMGEIAARLMPAMRGSRSDPEGLAHAERVMAAVPPD
                     TYRKAVHLLTTFDRREQLAGIRVPTLLLAGGDDRVAPAAVMERMARRIPGAEFVLLEG
                     CGHLGPMDQPGPFNEALAAFLERHKL"
     gene            complement(9313..10350)
                     /locus_tag="AC16O9_01690"
     CDS             complement(9313..10350)
                     /locus_tag="AC16O9_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase NicX
                     family protein"
                     /protein_id="HIR6515364.1"
                     /translation="MTPGRIEGKWIDLFAEVFARCKVAPGDACAVLSESQSRTLNVQL
                     AELALHKLGARPFHLVVPTPRQQAPVPIRSTGASVALQGLGPALQALAAATFVADCTL
                     EGLMHAAELPAILKGGARVLYISNEHPDVLERLAPSSADEEKVRAGMRRLKDAKLMRV
                     TSTAGTDLTVRLEGARVGGVWGYTEKPGTLSHWPGGLCLCFPRAASVNGTLVMAPGDL
                     NLTFKRYAESPVRLSIDQDYVTRIEGDSLDGELMRAYFAAWGEREAYAVSHVGWGVNE
                     RARWDALVMYDRADVNGTEQRAFAGNFLYSTGANEVAGRHTLGHFDLPFRRCTVELDG
                     DAVVREGKLAA"
     gene            10380..10811
                     /locus_tag="AC16O9_01695"
     CDS             10380..10811
                     /locus_tag="AC16O9_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HIR6515365.1"
                     /translation="MKIFDLQCGSGHRFEGWFASADEFTVQRARGQVSCPSCGAQEVD
                     RVPSATRFNTGAPPPMAREEAGKDPLALAQKLYSRMLDELLKHSEDVGAEFPAEARRI
                     HHREAPERMIRGQATQEEHAELIEEGIPVLRMPIPPAKDWN"
     gene            complement(10894..11859)
                     /gene="holB"
                     /locus_tag="AC16O9_01700"
     CDS             complement(10894..11859)
                     /gene="holB"
                     /locus_tag="AC16O9_01700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR6515366.1"
                     /translation="MNNKLYPWLDEAWKALQSKLARMPHALLIHGPQGIGKQQLAERL
                     AQRILCESTAGGEPCGACPGCRWFAAGSHPDFRRLEPEALARAPAEDTEDAAPTRTAK
                     PSQEIKVDQVRALDGFLNLQSHRGGPRVALIHPAEAMNPNAANALLKGLEEPPGRAQF
                     ILVSHRPSRLLPTLRSRCVALPVAVPERGVSLAWLEGQGTRDAASWLAFASGSPLRAL
                     QQQAGGTLARRRQALEARDREALGLAIDREQLEELAEVLQKHALDRAFVAFGGRSKFG
                     TARETKAGTEWLRYARLMGRNRALARHPLNPRLFAYEMLAAMPRE"
     gene            complement(11856..12467)
                     /gene="tmk"
                     /locus_tag="AC16O9_01705"
     CDS             complement(11856..12467)
                     /gene="tmk"
                     /locus_tag="AC16O9_01705"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR6515367.1"
                     /translation="MTRGRFVTLEGVDGAGKSTHVAWIAERLRVPGRKVLVTREPGGT
                     PLAERLRALVLTEPMDPIAETMLLFAARADHVQREIAPALAAGDWVLCDRFTDATFAY
                     QGGGKGVAAELIAHLAQVSHDGLLPDRTLVFDCPYEVSRERLNRAARTPDRFEREDRD
                     FFERVRQAYLLLARSDPERIRLIDASGAPEDVKKRVEEEITGL"
     gene            complement(12464..13468)
                     /gene="mltG"
                     /locus_tag="AC16O9_01710"
     CDS             complement(12464..13468)
                     /gene="mltG"
                     /locus_tag="AC16O9_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6515368.1"
                     /translation="MPRRLRIYLLLAVFAALAAGAYAAWYVHAAVAVASLPVEFEVPA
                     GSSLRATVRSLEKAGLVTRPLHFELYARLTGRAGEIKAGNYEITAPASPSALLDKLTR
                     GAVMQAELRIIEGWHVGQLRAALDASPFLRHDTEGLTDAELLARVGATERHPEGLFFP
                     DTYLFAHGSSDLAVLRRAYHAMQRHLDDEWAQRAADVPYKTRYQALVMASIVEKETGR
                     AEERDRIAGVLVNRLRIGMALQADPTVIYGLGKDFDGNLKRTHLTTDGPYNTYMRAGL
                     PPTPIALPGLASLRAALRPAPTKALYYVSRGDGTSQFSRNLEEHNRAVSKYQLQPRRK
                     "
     gene            13504..14421
                     /gene="ygfZ"
                     /locus_tag="AC16O9_01715"
     CDS             13504..14421
                     /gene="ygfZ"
                     /locus_tag="AC16O9_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR6515369.1"
                     /translation="MDTKVSYSAAELLRLGLLSCMGDEARGFLHAQLTNDIAGLAQDE
                     ARAAGWCSAKGRLLATFLVMPHDGGFLLQLPRELAAPVGKRLSMFILRTKAKLADVSE
                     AWAQFGVWGSAAAERLAALGFDVPQAPMRVARSLDGVVVQVAQQRFLVLLPVARRDRI
                     AALACTAADDAWALEEIRGGRPQVLAATQDQFVPQMVNLERLGAVDFRKGCYPGQEIV
                     ARTQYRGVLKRRMVRARVAAAAAPGDELFAEELPGQASGVVANAAASPEGGSELLAVA
                     QISALESGRPIRLRTADGPVLELLPLPYA"
     gene            14418..14675
                     /locus_tag="AC16O9_01720"
     CDS             14418..14675
                     /locus_tag="AC16O9_01720"
                     /inference="COORDINATES: protein motif:HMM:NF027614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HIR6515370.1"
                     /translation="MTSFFVYYRVDAGRMPALRQDVMRLFAAIESATGVRGRWLRRRD
                     EPSTYMEVYENVSDDDAFEALLAREAATLGLERHVERFVCA"
     gene            14666..15325
                     /locus_tag="AC16O9_01725"
     CDS             14666..15325
                     /locus_tag="AC16O9_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR6515371.1"
                     /translation="MCLILVGWRMHEAYPLVVAANRDEFHDRPAARAAFWDDKPDVLA
                     GRDLQARGTWMGVSRDGRFAAVTNYRGGTEPGAAESRGALVMRYLEGAGIPQLAERGA
                     AYSGFNLVASDGTELWWLSNRDGGPRRLEPGWYGLGNLLLDSPDVEAHKERFRASPAS
                     IEPLFAVLAPARIVDARYGTRCSSVLLQGVETRFAERGFDREGAEQATLRYEFTTRRI
                     S"
     gene            complement(15295..15717)
                     /locus_tag="AC16O9_01730"
     CDS             complement(15295..15717)
                     /locus_tag="AC16O9_01730"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6515372.1"
                     /translation="MPHIELFDWETARAEASRIRMTVFVQEQGVPAHIEMDDMDAACA
                     HALAYVAGKAVGTGRLLPDGHIGRMAVLKECRALGVGGAILVSLMAEARRRGMKEVVL
                     SAQTHAIGFYRKHGFREVGAVFEEAGIPHQEMRRVVNS"
     gene            15741..17711
                     /locus_tag="AC16O9_01735"
     CDS             15741..17711
                     /locus_tag="AC16O9_01735"
                     /inference="COORDINATES: protein motif:HMM:NF006196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HIR6515373.1"
                     /translation="MKSLWNDAEAAQYTGPLGPRVYTSRLLGRDKSLVLHGGGNTSVK
                     LREKNLFGDEEQVLYVKGSGWDMETIEAAGFAPVKLDYVRRLASLPALSDPQMVNELA
                     THTLRAGAPAPSVETILHAILPHKYVDHTHADAVLSISNSPEGEARARDIYGDKAVII
                     PYIMAGFDLASYCAREFPKQAKKGTVGMVLLSHGVFSFAEDARESYELMIELVSMAEK
                     YLAARKAWSLGPPPATASGFAREDAARLRRDVSHAAGFPLVCLCNDTPRFLGFARHPE
                     VQRLAQGSPATPDHVIRTKPVPMLGRDVAGFGAAYRSYFERNAAKAKEPKTMLDPAPR
                     MALDPQLGFAAFGRSARDARIVEELYEHTIDVILRAEALGGWRPLDDRHIFDIEYWDL
                     EQAKLRKGGTPAAFAGEAALVTGAASGIGRACVEALLRRGAAVVGVDRNAAIETLFKR
                     PDYLGVVCDLTDASAIDSAVTAAVRRFGGIDMLVLNAGIFPSSAPIQSLSIASWRSAM
                     TVNVESNLLVMQAVHALLALAPRGGRVVVIGSKNVPAPGPGAAAYSASKAALNQLARI
                     AALEWAKEGIRVNTIHPNAVYDTALWTEEVLASRAKAYNMTVEQYRKNNLLKTEVSSK
                     DVAELAAEMCGPLFAKTTAAQVPVDGGTEKLV"
     gene            17708..18667
                     /gene="mtnA"
                     /locus_tag="AC16O9_01740"
     CDS             17708..18667
                     /gene="mtnA"
                     /locus_tag="AC16O9_01740"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR6515374.1"
                     /translation="MTVEALRWKGDRLEVLDQRLLPDRVAYLTCRTAAEVAEAIRGMA
                     VRGAPAIGCAAAFGIALEPKESSYDTLAKSRPTAVNLFWALERMRGAKDKRAEAEAIF
                     AEDLKINRAIGANGAKLIPPGARILTYCNTGALATAGHGTALGVVRSAKSKGISVLAC
                     ETRPYLQGARLTAWECAQEGIPCTLIADNMAGHLMSRGEVDLVIVGADRIAANGDTAN
                     KIGTYTLAVLAQRHRLPFYVAAPLSTFDAHTPEGSKIPIEERAAEEMTGYRGARWAPE
                     GVRVRNPAFDVTPAELVTAIICEKAVVHAPSRERLQPLMSTSA"
     gene            complement(18640..19104)
                     /locus_tag="AC16O9_01745"
     CDS             complement(18640..19104)
                     /locus_tag="AC16O9_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6515375.1"
                     /translation="MRVEIDVARQELRLPDGRRYAVSTAKNGLGEKNGSFCTPRGRHI
                     VRAKIGAGQPLGAVFVRRRPTGEVWSPELHAKYPGRDWMLTRILWLSGCEVGRNRLGD
                     VDTMRRMIYIHGAPDSAEMGTPGSHGCIRMRNADVAELFELVPPYAEVDIRG"
     gene            complement(19101..19526)
                     /gene="tadA"
                     /locus_tag="AC16O9_01750"
     CDS             complement(19101..19526)
                     /gene="tadA"
                     /locus_tag="AC16O9_01750"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR6515376.1"
                     /translation="MSDAQFMRRALELALRTEDEVPVGAVVVLDGRVVGEGWNRPVST
                     QDPSSHAEIEAMRAAGKATGNYRLTGATLYVTLEPCVMCVGAAFHARIARVVYGAPDP
                     KTGASELFMSRFNHHARVQGGVLAEECGAALKAFFAARR"
     gene            complement(19531..20031)
                     /locus_tag="AC16O9_01755"
     CDS             complement(19531..20031)
                     /locus_tag="AC16O9_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR6515377.1"
                     /translation="MSLTLTEFLAHAVAMEQEAADRYLELADMMEAHRNDAAAALFRD
                     MNRFSILHRDSILERAGTLELPRLNSWEYRWRSPPEVGGEEAFDYLLEPYNALRYARQ
                     NEVRAMKYYRSVAAEARDSELRRMASEFAAEEREHAQSLDEWIARTPRPSMTWEEDPE
                     SLEPLD"
     gene            20127..20861
                     /locus_tag="AC16O9_01760"
     CDS             20127..20861
                     /locus_tag="AC16O9_01760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HIR6515378.1"
                     /translation="MIEPLLLAVARVSTFRQQQPLTNASGFFFERDERLFLVTSRHVV
                     SDAPAQHFPDRLEIELHIDAADMAKSAAVSIPLFREGASLWRQGVDAAGEIDVAVIEI
                     ERSTLPVTAVFHAFSPAHLCDLETDRVEVGSSLLIVGFPLGFKDTLHHMPVVRRAALA
                     SAFGLRFNGEGYFLTDARTHRGTSGAPVVLRAATMAPALGKLQLKLLGVHSARVDAGS
                     RDREMDEALGLNTAWYADILLTLTEG"
     gene            complement(20915..21238)
                     /locus_tag="AC16O9_01765"
     CDS             complement(20915..21238)
                     /locus_tag="AC16O9_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0000423 - mitophagy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR6515379.1"
                     /translation="MLLDVRTYTCRPGTIKAHLALYDKLGRTPQTRHLGQPLAYLVTE
                     SGNVNQYIHIWVYENAADREKRRAAMWADAEWIAYTQESAKLGALDAQENRLMTPVEF
                     FPLKR"
     gene            complement(21255..21497)
                     /locus_tag="AC16O9_01770"
     CDS             complement(21255..21497)
                     /locus_tag="AC16O9_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515380.1"
                     /translation="MRIHTNHLALRCLGTLVGLGFLAIGTYAMLGGDDAVGAHALERA
                     WWFGLTAVIAGAWAIGASWLDSDLSGVWCRPPRRFR"
     gene            21517..22047
                     /locus_tag="AC16O9_01775"
     CDS             21517..22047
                     /locus_tag="AC16O9_01775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6515381.1"
                     /translation="MARALAVAACAGLLGCAMRAPDPHDADVLYLPTPPAVVEAMLDL
                     ADIRPGDVLYDLGAGDGRIVIAAARRYGVRAVGIELDTRKVAEARANVARAGLGDRVE
                     IRHGDLFDTDLGEASVVTLFLFPELNARLAPKLRRELQPGTRVVSHRFGLGDWPPDRR
                     IEARGHPLLRWTVPPR"
     gene            complement(22044..22358)
                     /locus_tag="AC16O9_01780"
     CDS             complement(22044..22358)
                     /locus_tag="AC16O9_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HIR6515382.1"
                     /translation="MSVTPRERLVGAFRPHLYIYLAVNAGLTGINVATGAPWWAVWPL
                     VLWGMLLMLHFLYCRATTVDEAWVEERTLDLRSKSYDAGHIDDIRNRPARSIEEEGGG
                     SR"
     gene            complement(22355..22801)
                     /locus_tag="AC16O9_01785"
     CDS             complement(22355..22801)
                     /locus_tag="AC16O9_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515383.1"
                     /translation="MDKYFRKYVWDDERTPYLISVARLTRAQIEYELRFYAVITGVLF
                     ALVSLAALSQSLPHGDARIVSVYAFTQVCAALLLGMTRSAWAAFYCASAPLAALLYFA
                     LFGFHPSLGAGDKTLLVAAVVLWVAYGGRLIAIARAYAGLAEPDAR"
     gene            complement(22812..23531)
                     /locus_tag="AC16O9_01790"
     CDS             complement(22812..23531)
                     /locus_tag="AC16O9_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6515384.1"
                     /translation="MALRLSNRLRAFVDFVGRWGSWLVIPMVLFTIVDVVGRKIHWLD
                     DEGKQHGMQVFLVSVFGRIFESTMLQELEWHFHAGLFALVLGYGYIHNSHVRVDLIRE
                     NLAFRKKAWLELFGITFFLIPFTLTILYFAVTYAYGSFQMNEQSTSTVGLTHRWIIKS
                     VLVFGLLTVVTAGLAVWLQVALVLFGDPARRFPLMTFEWPEEDAKIEGKERVKLEDAP
                     NTLVAPDAALKERTSKILTGG"
     gene            complement(23614..>23901)
                     /locus_tag="AC16O9_01795"
     CDS             complement(23614..>23901)
                     /locus_tag="AC16O9_01795"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR6515385.1"
                     /translation="PVFAPIVATLDFGEHVPKAEITYWFIVLMAVNLQTSFLTPPFGF
                     ALFYLKGIAPKEVSIQSIYKGIIPFVLLQLTAVIIVLAFPDVALWIIRANN"
BASE COUNT         3843 a         8139 c         8222 g         3697 t
ORIGIN      
        1 gggcggcgag gaaacaggct gaggctgatt gaggcagcct gaaatgcctt actaggtgcc
       61 ttaaaatccc tcggccgcaa ggccgtgccg gttcaagtcc ggccccgggt acccgctcag
      121 gccgccgcca gggtgagacg gatgcgattg cgaccgaaca ggtaggcgta gtcgcgcgac
      181 gcggccagct cccttgtgag aagcacaccc agaccgccga tcttgcgcat cgacacggtg
      241 gtatccacga ccgcttcggt caggtgggcg taggggttga agggcggcgc cttgtcctcg
      301 tagaccacgg acaacgtgtc gccgagccga gacactgcga cccaaaccgg tgcatcgcag
      361 tcgccgcggt ggccgtgctt cacggtattg gtaaacaact cttcgaggac gagattcagg
      421 cgtaaatatg ccgcgcgcgg aaggttcgat tctgcgcaga attgctccag aaaccgctgc
      481 atcgggcgca gctggtcgag gcgtgcaaga aactgtgctg ctcggtcgta tgtcacggct
      541 ttacccaatg ccctcagggc attatagaat ccttgtcact cagcaggagg gcgtatgcgc
      601 cattttcttg cggtagcggt tctggcactg gcggtcacgg gtttcgcgcg ggcgcaggac
      661 gtcggccatg tcaaggtcgc gaagggcacg gtgcacgtcg agcgcgacgg caagcgcctg
      721 ccggccgcgg tgggcatgac gattcgacag tccgacacgg tgctgaccgg cgcggacggc
      781 tccgccggcg tgaccttcag cgataacagc ctcctttcca cgggccccaa cagcgtgctg
      841 gtcgtggatc actacgcctt cgattcaacg acccacgccg gcaagttcga cgcgtcgctg
      901 aagaaaggta cgctggcggt ggtatccgga aagatcgtga agcagtcccc cggcgcgatg
      961 cgcgtgcgga ccccggcggc gatcatgggc gtgcgcggca cggaattcat ggtgcaggtg
     1021 gacgagccgg caaagtgaaa tgggggcggt aattgtcgcg ctcgtcggga cgctcatggt
     1081 ctttctcgcc cccgatcgcg tgggtaatga attggtcgtg gtggttccct cggcagatgg
     1141 ccacgtcggc gtggtgatcg tcgagcgagg cgacaagaag ctgaccctgg acacggcttt
     1201 cgctgcgagc cgcatcgtcg atggcggtgc acccgccgcc gagcgcttcg gcggcgccga
     1261 cgcggtgcgc gccgaatttg ccgacgtcct tgctgcgctg cccgaccgac cgcagtcctt
     1321 cctggtgtac ttcctggaag ggacggacga gttcaccgag gaatccggta tcgagttcac
     1381 gaagatcctg tccgagctgc gcgagcgcag cgctcccgac gtggtcgtca tcgggcacac
     1441 ggatcgcgtt ggcaatgttc agtacaacga cgatctgtcg ctgcagcgcg ccgagcgcgt
     1501 gcgtggcgag ctggtcaggc tcggcatccc gccggcgcgc attcgcgtcg ccggacgcgg
     1561 cgagcgagag ccgttagtgg cgaccgacga cgacgtcgcc gagccccgga accggcgcgt
     1621 cgagatcaac gtccgctgaa tcgtccgggc ctgccggcag cgcggcagtg ccgttccacc
     1681 gtacgcagag cagggtcaga tcgtccgagg cttcagccgc gccgacgaag cgaccgacat
     1741 cggcgcgcac ggccgccagc agctcgctcg gcccgacgcc ggccggcagc gcctccagca
     1801 ccgatcgcag tcgcgctgcc ccatagagct cgcccgctgc attcatggcc tcggtgacgc
     1861 cgtcggtcac caagcagatc cactcccccg cggcgagcgc gcgatgctcg atcggatagg
     1921 cgtagttctc catgacgcac aagggcggtc cgcccgcgtg caccagccgc tccagggcgc
     1981 cgccaggagc gcgcgcaaag ggcggctcgt gccccgcatt gcagaactcc agcatgccgg
     2041 tgcgcgcatc gagcaggccc gcgaacgccg tgacgaacaa ggactccgga ttctcgctcg
     2101 acacctcggc attggcgcgg accatcagcc ccccgacgtc gccgccgcgg cgcgccgcgc
     2161 tcttcaggag cgtcttggac agcgccatga acaggctggc cgggagtcct ttgcccgaga
     2221 cgtcggcgac gatgaagaac aggcgctccg aatccaccat gaagcagtcg tagaagtcgc
     2281 cgcccacggt gcgcgccggc tccagcagcg catcgatggc gaagcgctgt tcgtgcgcga
     2341 acaacgcgcg gggtgcgggc agcagcccca tctggatacg gcgcgccgat tcgagctcgc
     2401 cgctgacgcg ggccgcctgc tcgcgcagcc ggcgccgctg ctcgtcggct tcgctcagcg
     2461 cctgcgccag aacgatgccg aagacgacgg tggcgccgat cgccggccag gcaacgtcga
     2521 gcagcaggcc ttcgagcaga aagccctcga tgcccagcgc cgtgagcagc gcgatgacca
     2581 ccgcgtacag ggcgatcgat gtggccacgc cgagcgaggg caccgtcagt gcaaacatca
     2641 gcgccgcgac cgcgagcaag ccaagctcca gcgcatgcag ggtggacgga cggcgcagga
     2701 aggccccgtc gaagatctgc tccaggatct gcgcatggat ctccgcaccg gggatgcgct
     2761 cgccgagcgc ggtggtctgg taatcgagca ggcccagacc ggtgacgccc accagcacca
     2821 gcttgtcctg gagctcgggc gctgcgacgc taccctccag gacctgcgcg gcggaaacaa
     2881 agcgcgccgg atcgtgtggc ccgtagcgga tccagaagcg gccatcggca tgcgcgggaa
     2941 tggcgatgtc gccaaggcgc acttcgacct ggtcgcggcc gcgtgcgatc agtgcgagcg
     3001 gcgcgccggt cgccacgcgc agcatctcca cgctcagcgc cgggacgatg acgtcgccga
     3061 tgcgcgcggc ggcgagcgcg cggcgaatta cattgccctc cggatcgacg ctgagcaggc
     3121 ctcggccggc ggcggcacgg tcgatctcgg gcaggctctg caggtgtccc gcgaagcggc
     3181 gcagggcaaa gtcggcgccg tcggtgatac ggatcggcgc cgccgcgggc gggcgctcga
     3241 aacgcggatc gggggcttcg aggccggcaa tacccagcac cacggcgcgc gccttcaatg
     3301 cggcggccag ggcggcgtcg ttggagggga gggcgcgcag ccgccgtgcc acatccacgg
     3361 acagttcctg cacctcgcgc gcgacttcgg ccggggagaa gcgatcgggt tccgcaaaca
     3421 gcaggtcgac gccgatcgcc gccgggccgg cgtcggaaat gccggccagc agttgcgcga
     3481 cgcgcgtgcg tggccagggc cattggccgt ggcgctcgag cgcctgctcg tcgatggcga
     3541 cgatgacggc tggggcgctg atgcgctccc tggggaacag gcgctggtag ccgtcgaaca
     3601 gggcgtgccg cagcccgaat acgaaaggcg gttcgctcgc gagcaccagc agcagcgcga
     3661 tgaccagcgc cagcggcccg gcccgcttga accaggcgga actcacacgt cgatctccag
     3721 gccgtcgtag gccgccgaga tccgcagcgg ctgtgtacct cgcgtcagtt cctcgaagcg
     3781 ccgagtttcg cgcagcacgc cttcgatcgt tgcgtcgtcg taggccggct cgtggtggaa
     3841 cagcacgaga tgcttcgcgc gggccatctg gcagagctcg acgccgatga tgttgctcga
     3901 gtgtccccag tccgccttca ccgagatcgc gtcggcgagc gagtacatgg cgtcgaacac
     3961 caccaggtcg gcgccctgga agaagcgcac gaagcgctcg gtctgcgcgc gattctccag
     4021 cttatgctcg gagtcggtgg tgtacacgac gaccttgccg ccgacttcga agcggtagcc
     4081 gaaggattcc cccgaatgca tctgcggcgt gggcgtgacc cggatgccgg agaattcgtg
     4141 ggggcgcccc ggtgccagct tcacgaactc tatgtcggcg gcgagctgcg agaattcgac
     4201 cggaaagcag ggcggcgcct gctgcagccg gaacgcctgc tcgatcacgt cgtggcaacc
     4261 atggatgcgg atgtgcgtgc cgggcacgta ggcgggcccg aagaacggaa aacccatgat
     4321 gtggtcccag tgcacgtgcg acatgaagac gttcacggtc gccggcttgc cggactggcg
     4381 cgccatgacg tgcgcgccga atggtcgggc accgctgccg aggtcgcaga ccaggtattc
     4441 ctgcgcgccg gtttccagct cgacgcaggg cgaatggccg ccgaaggtgt gcgtcaggga
     4501 gaaatcgagg tgcttgagcg cgaaatcatg cgcttcgccc accgattcga agcggcgccc
     4561 ctctgccttg aacagcgcca gggcaacctt gtcgcggatg tccgcggagg tcagcgcaac
     4621 gggaatcgag ccgcgcgtgc cccagaagcg gacgcgcacc cgcgctatcc ccggtcgaac
     4681 gcggcggcag cggcgggcga cagcgcggtg agcgcgtcgc gcaccttggg gaaagcctcg
     4741 aacacgaggt tgaagcgcgc aatctggaag atttcagcca ccaccggctg catcgcggcg
     4801 atcacgatgc ggctgttctg cgccttggcc tgcttggcgg cgagcatgaa gcagcgcaat
     4861 ccggcgctcg agacgtattc caggcccgac aggtcgagga ccagggacat gccctccttg
     4921 gccgagcgct cgaggtgcgg cgccagttct tcgcggaagg cttcgcagtt gtcgtgatcc
     4981 agtcgcccgg tgggcgtaag caccagggcg ttgtcgtaga ggcggtgggc aagaggcatg
     5041 cgaggtcctt ccggcgccta ttgtctgcaa agccgggacg ctcggcaacc gctagaatcg
     5101 aactcatgcg cgctgacgac gccagccgtg tgcatcgagc cgcgcgctgg ctcgtgcgcc
     5161 agcaccaggg gcgactggca tcccggccga tgtcacgggg gctgacgccg cgcacagctg
     5221 atgaggcgta cgcggtgcag gacgccttcg tccggatgaa agcgcgctac tgcggccgcg
     5281 ttaccggcca caagattgcc ctgaccacgc cgcgcatgcg cgccatggtg gggctggaga
     5341 cgccgatcgg cgggcagctg catcagcgcc aggtcgtgcg cgggccggcc agcgtgcaag
     5401 ccgctcagta cgggcgcctg atcgtcgagt tcgagatcgg gatgcagatg gcgcatgacc
     5461 tgccgcccga gcgcgcgccc gtcacgatgg ggcaggccgc cgatgcggtg ggggctgtca
     5521 tgccggcgct cgagctggcc gatgaccgca acgcggacta cgccacgttg tcggcgcgcg
     5581 gcctcgagct ggccgccgac aacgcgtgga acgaaggcgc cgttctcggc gccccggtcg
     5641 acgactggcg tgacgtggac ctcgccggac tgcgtggcag cgcgctgatc aacggcgcgg
     5701 tggtcggcga gggccgaggc gccgacagca tggggcatcc gctggccgcc ttggcgtggg
     5761 tcgcgaatca tcttgcgggc cggggccagg gattgcgccg cggcgatgtg gtgatcacgg
     5821 gcagcctggt gacgtccaag ttcccgcgat ccggcgaccg ggtgcgcttc gaggcgggcg
     5881 cgctcggcgc ggtcgagctg caggtggcct agcgcgactg cagcagcttc cgcagctcgt
     5941 tcttctgcac cttgccgagg gcattcttcg gcagggcatc cgcgaatact atttggcgcg
     6001 ggtgcttgta gcgagccagg cggtccttga acagccccag cacgtccgcc tcggtgagcg
     6061 tggcgcctgc ccggcgcacg acgacggcgc aggcggcctc gccccatttg tcgtcctgca
     6121 cgccgatgac cgtcgattcg gcgatcgccg ggcagtccgc gagcacgctt tccagctccg
     6181 cgggatagat gttctcgccg cccgagatga tcacgtcctt ggagcggccc atgatccagt
     6241 aatggccttc gtcgtcgacg cgcgcgaggt cacccgaatg gaaccagcct tcggcatcga
     6301 gcacgcgggc ggtggccacg gggtcgttcc agtagcggcg catgacgttc ggcccgcgca
     6361 cgcagatctc gccgtgcacg agcttgattt cgcagtggca tgccggcttg ccggtgctgc
     6421 cgaccttgcg cagcgcatcc tcgcgggtca gcacgagcgc aatgggcgca gtctcggtgg
     6481 agccatagat ctggcccacg ggcacgccgc gctggtggaa cgcgtggatc agggactctg
     6541 ccacgaccat ggagccggtc atcagcagcc gcaggctgga cagatccgtg cgcgcccagt
     6601 ccgggtgtgc gatgacggcc tgcatggtcg cggggaccag aagcgaaagg ctcggtcgtg
     6661 ccgcgtgcac ctcgcgcagc cagcgcccgg gctcgaagcg cttgtgcagc gtgaccgtcg
     6721 cgccggcgag cagagcggga acggtctggt tgttcagccc gccgacgtgg aacatcggca
     6781 gcgtgctgag gacgtggtcc gcctggccga gtttgtgcgc gtggatcgcg ttgtagccgt
     6841 tccacagcaa ggccgactgc gccagcaccg cgcccttggg ccggccggta gtgcccgagg
     6901 tatagacgat gagcaggtcg tcctcgtcgg ctccggccga ggcggtgccc gcgggcgcat
     6961 cgagctgccc catcggaacg cccagcgcgc gcgccgcctc ggcgaagtcc gcgtcggcga
     7021 acatccagcg cgggcaactg tcgtcgagga tgccgcggtg ctccgccgcg gtcagccgcc
     7081 agttcagcgg aacgagcagg gcgccgcgtc gcgccgccgc gaacagcagc accagcatgt
     7141 cgggacggtt gtagccgagc caggcgatac ggtcgccctt cgcgacttcc agcgcgcgcg
     7201 cgacgtgctc gatccgctgc cacagctcgc cataagtcca ttgctcgccc tcgaagcgca
     7261 gcgcgatgcg ggatggtgca tgtcgcgccc attgctccac gatgcgcgag agatccacgt
     7321 cagtccactt cgccgctctc gtacagcgct tcgcggccga tgcgatccag gcacagctcg
     7381 gcggtccagg gcagcatcag ggcgccgcag cgcgagtcgc ggtacaggcg ctccagcggc
     7441 agcgacttca gcatcgattg cccgccgcag gtgcggatcg ccagccggca gatgtcgttg
     7501 gcattctcca tgatcgtgta ctgcgcggcg taggcgcgca ggcgcgcgtc cttgggcgga
     7561 tccacgcggg cgtcctccag catgcgcagg aacagcgcgc gcgtttgctc gagcctcacc
     7621 cacagctcgg ccacggcgat ctgtttcgtg gggtacatgc ggcgcttgac cggcggcatg
     7681 ccgtcgactt cgccgcgcaa gtagcgcacg gtgaactcgt acgcagcgat ggcgatgccc
     7741 atgtaggtcg gcgagagcgt cgtgaacatg tgcggccagc ggctggctgc ctgaaagtac
     7801 accccgcgcg gcatcatggc cccgtcgtcc ggcacgaaca cgtccttgaa taccagcgtg
     7861 cgcgaaacgg tgccgcgcat gccgagggga tcccagtcgc cgacgatgga gaatccgggc
     7921 gcatcgcccg gcacggccag gtacatcgta tcgcgcatcg acagctcggg cttgttctcc
     7981 gtgcacagca cgccgtagta gtccgccgct cccgagagcg aggcgaagat cttcttgcca
     8041 ttcaccagcc agccgccgtc gactttgtcc gcggtggtgc cgaagggcgc cttgccggcg
     8101 gccgcggcac tgccttcgga gaacggctgc gcgtacaccg cgccctttcg cacgacgcgc
     8161 tcgaagtgca gcgcgcgata gcgctcatgc tccgcgcgct gcgcctcggt catctcgagg
     8221 tcgtcggcga gcacgccggg ccacagagtg gagcaggcat gcatgttgaa ggtgagggcg
     8281 gtggcgccgc agtacttgcc gagcgtggcc gacaccaggg cataggtctt caggtcagcg
     8341 cccttgccgc cgtacttctc cggcacgcac aggccgagca tgcccgcatc gcgcagatcg
     8401 gcgtagttct ccacgggaaa gctcgcgtcg cggtcgtagc gctcggcgcg cccggcaaat
     8461 cgcggcccaa gcgcctcggc ttgcgcgagc agcgcctttt gcttgtcgct cagggggtaa
     8521 ttcgagccgg cgacggcgta gcgcgggtcg gattgcggaa ctctatcgtg catggctatc
     8581 ctcagagctt gtgacgctcg agaaaagccg cgagcgcctc gttgaacgga cccggctggt
     8641 ccatgggccc gaggtgtccg cagccctcca gcaggacaaa ctctgcgccc gggatcctcc
     8701 gcgccatgcg ctccatcacc gccgccgggg cgacccggtc gtccccgccc gccagcagca
     8761 gcgtcggcac ccggatgcct gccagctgct cgcggcgatc gaacgtggtg agaagatgca
     8821 ccgccttgcg gtaagtgtcg gggggcaccg ccgccatgac acgctcggcg tgggcgaggc
     8881 cctccggatc cgagcggctt ccgcgcatcg ccggcatcag gcgcgcggcg atctcgccca
     8941 tggacttgcc cgcgtcgagc ggcgcgatcc gggctgcgat gaactggcgc gcgaactctc
     9001 cgctgccgcc gccaaacgcc gcgctggtga aacacaggcc cagggcgcga atgcgatcgg
     9061 gaaagcgcgt gtaggcctcc tgcgcgatga agccgcccat gctatggccg acgaggaccg
     9121 ccggctcgtc gccgatgctg cgctcgagtg cagccgtcac ctgcgcaagg tcgtacggct
     9181 cgacgggcgc cgtgccccca tagcccggct ggtcccaggc gcgcgcgcga tacccgcgcg
     9241 cggcgaaata ggggagctgg cgcgcccagg cggcactgcc gccgccgacg ccgtgcagga
     9301 acagcagcgg gctcacgccg ccagcttgcc ctcgcgcacg accgcatcgc cgtcgagctc
     9361 cacggtgcag cgccggaacg gcaggtcgaa gtgccccagc gtatggcggc cggcgacttc
     9421 gttggcgccc gtggagtaca ggaagttgcc ggcgaacgca cgctgctcgg tgccgttgac
     9481 gtcggcgcgg tcgtacatca ccagcgcgtc ccagcgcgcg cgctcgttca cgccccagcc
     9541 gacatgtgaa actgcgtagg cctcgcgctc gccccaggcc gcgaagtagg cgcgcatcag
     9601 ctcaccgtcc agcgagtcgc cctcgatgcg cgtcacgtag tcctggtcga tcgacagccg
     9661 caccggcgac tccgcgtagc gcttgaaggt caagttgagg tctcccggcg ccatcaccag
     9721 cgtgccgttg acgctggcgg cgcggggaaa gcacaggcac agcccgcccg gccaatgcga
     9781 cagcgttccg ggcttttccg tgtatcccca gacgccgccc acgcgagccc cctccagccg
     9841 aaccgtgagg tcggtgccgg ccgtcgaggt aacccgcatg agcttcgcgt ccttgagccg
     9901 gcgcatgccc gccctcacct tttcctcatc ggcggaggag ggggccaggc gctcgaggac
     9961 gtccgggtgc tcgttggaga tgtagagcac tcgcgccccg cccttgagga tcgccggcag
    10021 ctcggccgcg tgcatcagcc cctcgagggt gcaatcggcc acgaatgtcg cggcggcgag
    10081 cgcctgcagc gccgggccca gcccctgcag cgcgaccgag gcgccggtgg agcggatcgg
    10141 caccggagcc tgctggcgcg gagtcggcac gacgaggtga aagggccgcg cgccgagctt
    10201 gtgcagggcg agctccgcaa gctgcacgtt gagcgtccgg gactgcgact ccgagagcac
    10261 ggcgcacgcg tcgccgggcg ccactttgca gcgcgcaaat acctcggcaa agaggtcgat
    10321 ccacttgcct tcgatgcgcc cgggggtcat gacggcgaca tcgtaataga atccgacaca
    10381 tgaagatctt cgacctgcag tgcgggtcag gccaccggtt cgagggttgg ttcgcctcgg
    10441 ccgacgaatt caccgtgcag cgcgctcggg gccaggtgag ctgcccctcg tgcggagcgc
    10501 aggaggtgga tcgcgtcccg tcggcgacgc ggttcaatac cggggcgccc ccacccatgg
    10561 ccagggaaga ggcggggaag gatccgctgg cgctggcgca gaagctctac tcgcggatgc
    10621 tcgatgagct cctcaagcat tccgaggacg tcggtgcgga gtttcccgcc gaggcacggc
    10681 gcatccacca ccgcgaggcc cctgagcgca tgatccgcgg ccaggccacg caggaggaac
    10741 atgcggagtt gattgaggaa ggcatcccgg ttctcaggat gccgatcccg cctgcaaagg
    10801 actggaactg agcactctgg ccaggtgtaa gttgatcatt gtgtattatg tcaaatagtc
    10861 ggttgctctt ccacgtggca ctgccgacct ccttcactcc ctaggcatcg ctgccagcat
    10921 ttcgtaagca aacaggcgcg gattcaaggg atggcgcgcc agggcccggt ttcgtcccat
    10981 cagcctcgcg tagcgcagcc attcggtacc cgccttggtt tcccgtgccg tgccgaactt
    11041 agaccgaccg ccgaaggcca cgaaagcccg gtcaagggca tgcttttgca gcacttccgc
    11101 cagctcctcg agctgctcgc ggtcgatcgc cagccccagc gcctcacgat cccgggcctc
    11161 cagcgcttgc cgccgcctcg ccagggtccc gcccgcctgt tgctgcaggg ctcgcaatgg
    11221 cgatcccgag gcgaacgcca gccaggacgc cgcatcgcgc gtgccttggc cctccagcca
    11281 ggcgagtgaa actccacgct ccggcaccgc caccggcaaa gcaacgcaac gactgcggag
    11341 cgtcggcagg agccgggacg gtctgtgcga tacgaggatg aactgcgccc gaccaggcgg
    11401 ctcctccaac cccttcagta gcgcattggc cgcgttgggg ttcatggcct cggccggatg
    11461 gatcagcgcc acgcggggac cgccgcggtg cgactgcagg ttcaggaagc cgtcgagggc
    11521 ccggacttgg tcgaccttga tctcctggct gggcttcgcg gtgcgggttg gcgccgcatc
    11581 ctccgtgtcc tcggccggcg cacgcgccag cgcctcgggc tccaggcgcc tgaagtccgg
    11641 gtggctgcca gccgcaaacc agcggcaacc ggggcaagcg ccgcagggct cgccgcccgc
    11701 cgtggactcg cacaggatgc gctgcgccag gcgctcggcc agttgctgct tgccgatgcc
    11761 ctggggacca tggatcaaga gcgcgtgcgg catgcgagca agcttggatt gcagcgcttt
    11821 ccaagcttca tccagccaag gataaagctt attattcata accctgtaat ttcttcctca
    11881 actctcttct taacatcttc cggagccccg ctggcatcga tcagtcggat gcgctcgggg
    11941 tccgagcggg ccagcaacag gtaagcctgg cgtacccgct cgaagaaatc gcgatcttca
    12001 cgttcgaagc ggtcgggggt gcgcgcggcg cggttcaggc gctcacggct tacctcgtag
    12061 gggcaatcga acaccagggt acgatcgggc agcaacccgt cgtgcgacac ctgggccaag
    12121 tgggcgatca gctccgccgc cacgcccttc ccgcccccct gataggcaaa agtggcgtcg
    12181 gtgaagcgat cgcacagcac ccagtcgcct gcggccaggg ccggggcgat ctcgcgctgc
    12241 acgtggtcgg cgcgcgccgc gaaaaggagc atggtctcgg cgatcggatc catcggctcg
    12301 gtgagcacca gcgcgcgcag gcgctctgcg agcggcgtgc cgcccggctc gcgcgtcacc
    12361 agcaccttgc ggcccggcac gcgcaggcgc tcggcgatcc aggccacgtg cgtggatttg
    12421 ccggcgccgt ccacgccctc cagcgtcacg aagcggccgc gcgtcatttc cttctcggtt
    12481 gcagctggta ttttgacacc gcgcggttgt gctcttcgag gttgcgggag aactggctgg
    12541 tgccgtcgcc gcgcgacacg tagtacagcg ccttggtcgg tgccggacgc agcgcggcgc
    12601 gtagcgacgc gagtcccggc aacgcaatgg gcgtgggcgg caggccggca cgcatgtacg
    12661 tgttgtacgg cccgtcggtc gtcaggtgcg tgcgcttgag attgccgtcg aagtccttgc
    12721 ccaatccgta gatcacggtg ggatcggcct gcagcgccat gccaatgcgc agccggttca
    12781 ccagcacgcc ggcgatgcgg tcgcgctctt ccgcccgtcc cgtctccttt tcgacgatcg
    12841 aggccatgac gagcgcctgg tagcgggtct tgtaaggcac gtccgcggcg cgctgcgccc
    12901 actcgtcgtc gaggtgccgc tgcatggcgt gataggcccg ccgcagcacc gcgaggtcgc
    12961 tcgagccgtg cgcgaaaagg tacgtgtcgg ggaagaacaa cccctccgga tggcgctccg
    13021 tggcgccaac gcgcgccagc aactcggcat cagtcaggcc ctcggtgtcg tggcgcagaa
    13081 aggggctggc gtcgagtgct gcgcgcaact ggccgacatg ccagccctcg atgatgcgca
    13141 gctccgcctg catcaccgcg cctcgcgtca gcttgtcaag cagcgccgag gggctcgccg
    13201 gcgccgtgat ctcgtagttg ccggccttga tttcgccggc gcggccggtg aggcgcgcgt
    13261 agagctcgaa atgcagcggc cgcgtgacca gccccgcctt ttccaacgat cgcaccgtgg
    13321 cgcgcaggct gctcccggcc ggcacctcga actcgaccgg caggctcgcc acggccaccg
    13381 cggcatgcac gtaccacgcc gcgtaggccc ctgccgcaag cgcggcgaaa accgcgagca
    13441 acaggtagat gcgcagccgg cgaggcatgg aaaacggcat agaatcgctg caggcattct
    13501 acgatggaca ccaaggtttc ctactctgcg gcggagttgc tgcggctcgg attgctgtcg
    13561 tgcatgggcg acgaggcgcg cggctttctg cacgcgcaat tgacgaacga tatcgccggg
    13621 ctcgcgcaag acgaggcccg cgccgcgggc tggtgctcgg cgaaaggacg tctgctcgca
    13681 acctttctcg tcatgccgca cgacggcggc tttctgctgc agctgccgcg cgagctggct
    13741 gcgccagttg gcaagcgcct gtcgatgttc atcctgcgca ccaaggcaaa gctcgcggac
    13801 gtcagcgagg cctgggcgca attcggcgtt tggggcagtg ctgcggccga gcgcctcgcc
    13861 gcgctgggat tcgacgtgcc gcaggccccg atgcgggttg cgcgctcgct cgacggcgtg
    13921 gtcgtccagg tggcgcagca gcgctttctc gtgcttttgc cggtggcgcg gcgcgaccgc
    13981 atcgcggcgc tcgcctgcac ggccgctgac gacgcctggg cgctcgagga gatccgcggc
    14041 ggccggccgc aggtcctcgc ggcgacgcag gaccagttcg tgccgcagat ggtcaacctc
    14101 gagcgcctcg gcgcggtgga cttcagaaag ggctgctatc ccggccagga gatcgtggcg
    14161 cgcacgcagt accgcggcgt gctcaaacgg cggatggtgc gcgcacgcgt ggccgcggca
    14221 gcagcgccgg gcgatgagct gttcgccgaa gagctgcccg ggcaggcgag cggcgtggtg
    14281 gccaatgccg cggcctcgcc cgagggcggc agcgagctgc tcgcggtggc gcagatcagc
    14341 gcgctggaaa gcgggcgccc gatccggctg cggacggccg atggtcctgt gctggagctg
    14401 ctgcccctgc cctacgcgtg accagctttt tcgtctacta ccgggtcgac gcggggcgga
    14461 tgcccgcgct gcgccaggat gtgatgcgcc tgttcgccgc gatcgaaagc gccaccggcg
    14521 tgcgcgggcg ctggctgcgc cgccgcgacg agccgagcac ttacatggag gtctacgaga
    14581 acgtgagcga cgacgacgcg ttcgaggcgc tgctcgcgcg ggaggcggcc acgctcggac
    14641 tcgagcgcca cgtggagcgc ttcgtatgtg cctgatcctc gtcggctggc gcatgcacga
    14701 ggcgtacccg ctcgtcgtgg ccgccaaccg ggacgagttc cacgaccgac cggcggcgcg
    14761 cgccgcgttc tgggacgaca agcccgacgt gctggccggg cgcgacctgc aggcgcgcgg
    14821 cacctggatg ggcgtctcgc gcgacggtcg cttcgcggca gtcacgaact accgtggcgg
    14881 cacggagccg ggcgccgccg agtctcgcgg cgcgctggtg atgcgctacc tcgagggcgc
    14941 gggtattccg cagctcgctg agcgcggggc ggcctatagc gggttcaacc ttgtggcgag
    15001 cgacggcacg gagttgtggt ggctatcgaa ccgcgacggc ggaccgcggc gcctggagcc
    15061 gggctggtac ggcctgggca acctgctgct cgactcgccc gacgtcgaag cgcacaagga
    15121 acgctttcgc gccagccctg cttccatcga gcctttgttc gcggtcctcg cgccggcgcg
    15181 catcgtcgac gcgcgctacg gcacgcgctg ctcgagcgtg ctcctgcagg gcgtcgagac
    15241 gcgctttgcc gagcgcggct tcgaccgcga gggcgctgag caagcgacgc tgcgctacga
    15301 gttcacgacg cggcgcatct cctgatgcgg gatcccggct tcctcgaaaa ccgcgcccac
    15361 ttcgcgaaac ccgtgcttgc ggtaaaagcc gatcgcgtgc gtctgcgccg atagcaccac
    15421 ttctttcatc ccccggcgcc gcgcttcggc catcaggctg acgaggatcg cgccgccgac
    15481 gccgagcgcg cggcactcct tcagtaccgc catgcgcccg atgtgaccgt cgggcagcaa
    15541 ccggccggta ccgaccgctt tgccggccac ataggcgagc gcgtgcgcac acgcggcgtc
    15601 catatcgtcc atttcgatgt gcgcgggcac gccctgctcc tgcacgaaca ccgtcatgcg
    15661 gatgcggctc gcctcggcgc gcgctgtttc ccaatcgaac agttcgatat gcggcatggc
    15721 gcaaaagtat aattcgcagc atgaagagcc tgtggaatga cgccgaagcc gcgcaataca
    15781 ccgggccgct cgggccgcgc gtctacacct cgcgcctgct cgggcgcgac aagtcgctcg
    15841 tgctgcacgg cggcggcaat acctcggtga agttgcgcga gaagaacctg ttcggcgacg
    15901 aggaacaggt gctctacgtc aagggcagcg gctgggacat ggagaccatc gaagccgccg
    15961 gctttgcgcc ggtgaagctc gattacgtgc ggcggctcgc ttccctgccc gcgctgtccg
    16021 atccgcagat ggtgaacgag ctcgccacgc acacgctgcg ggccggcgcg ccggcgccgt
    16081 cggtcgagac gatattgcac gcgatactgc cgcacaagta tgtcgatcac acgcatgccg
    16141 acgcggtgct atcgatatcg aactcgcccg aaggcgaggc gcgtgcgcgc gacatctacg
    16201 gcgacaaggc ggtgattatt ccctacatca tggccggctt cgatctcgcc tcgtactgcg
    16261 cgcgcgagtt cccgaagcag gcgaagaagg gcaccgtggg gatggtgctg ctctcgcacg
    16321 gggtgttctc gttcgccgag gacgcgcgcg agtcctacga gctgatgatc gagctggtga
    16381 gcatggccga gaagtacctg gcggccagga aggcctggag tctgggtccg ccgccggcaa
    16441 cggccagcgg cttcgcacgc gaggacgcgg cccggctgcg ccgcgatgtg tcgcacgcgg
    16501 cgggctttcc attggtttgc ctttgtaacg acacgccccg ctttctcggc tttgcacgcc
    16561 atcccgaggt gcagcgcctc gcgcaaggca gcccggcaac gccggatcac gtcatccgca
    16621 ccaagccggt gccgatgctg ggccgtgacg tcgccggctt cggcgccgcg taccgctctt
    16681 acttcgagcg caacgcggcg aaggccaagg agccgaagac gatgctcgat ccggcgccgc
    16741 ggatggccct ggatccccag ctgggcttcg ccgcgttcgg gcgcagcgcg cgcgacgcgc
    16801 gcatcgtgga agagctctac gagcacacca tcgacgtgat cctgcgcgcc gaggccctcg
    16861 gcggctggcg gccactcgac gaccggcaca tcttcgacat cgagtactgg gacctcgagc
    16921 aggcgaagct gcgaaagggc ggcacgcctg ccgcgttcgc gggcgaggcg gcgctcgtga
    16981 cgggcgcagc gtctggaatc ggcagggcgt gcgtagaggc cctgctcagg cgcggcgcgg
    17041 ccgtggtcgg cgtcgaccgc aatgccgcga tcgaaacgct gttcaagcgt ccggattatc
    17101 tcggtgtggt ttgcgacctg accgatgcgt cggcgatcga cagcgcggtc accgcggcag
    17161 tgcgccgttt tggcggcatc gacatgctgg tcctcaacgc cggcatcttc ccctcctccg
    17221 cccccatcca gtccctgtca atcgcgtcat ggcgcagcgc aatgacagtc aacgtggaga
    17281 gcaacctgct cgtcatgcag gcggtgcatg cgttgctcgc gctcgccccg cgcggtggcc
    17341 gcgtggtggt gatcggctcg aagaacgtgc ccgcaccagg ccccggggct gcggcctact
    17401 cggcgtccaa ggcggcgctc aaccagcttg cgcgcatcgc cgcgctggag tgggcgaaag
    17461 aaggcatccg cgtcaacacg atccacccga acgcggtcta cgacacggcg ctctggaccg
    17521 aggaggtcct ggcttcgcgc gccaaggcgt acaacatgac cgttgagcaa taccggaaga
    17581 acaatctcct caagaccgag gtgtcctcca aggacgtcgc cgagcttgcc gccgagatgt
    17641 gcgggccgct cttcgccaag accaccgccg cgcaagtgcc ggtggacggc ggcaccgaga
    17701 agctggtgtg acggtcgagg cgttgcgctg gaaaggcgac cggctggagg tgctcgacca
    17761 gcgcctgcta cccgatcgcg tcgcgtacct tacgtgccgt acggccgccg aggtggccga
    17821 ggcgatccgc ggcatggcgg tgcgcggtgc gcccgccatc ggctgcgcgg cggcgttcgg
    17881 catcgcgctc gagccgaagg aatcgtccta cgacaccctc gctaagagcc ggcccacggc
    17941 ggtcaacctg ttctgggcgc tcgagcgcat gcgcggggcg aaggacaagc gcgccgaggc
    18001 cgaggcgatc ttcgcggagg acctgaagat caaccgggcg atcggcgcca acggcgcgaa
    18061 gctcatcccg cccggcgcgc ggatcctgac ctactgcaac accggcgccc tggcgaccgc
    18121 gggccacggc acggcgctcg gcgtggtgcg ttcagccaag agcaagggca tctccgtgct
    18181 tgcctgcgag acgcgaccct acctgcaggg cgcgcggctc acggcctggg agtgcgcgca
    18241 ggagggcatc ccgtgcacgc tgatcgccga caacatggcc gggcacctga tgagccgcgg
    18301 cgaggtcgat ctggtgattg tcggcgccga ccgcatcgcc gccaacggcg acacggcgaa
    18361 caagatcggc acctacaccc tcgccgtgct ggcgcagcgc catcgtctgc ccttctacgt
    18421 cgccgcgccg ctctcgacgt tcgacgcgca caccccggaa ggctccaaga tcccgatcga
    18481 ggagcgggcc gcggaagaga tgacaggcta ccgcggtgcg cgctgggcgc cggaaggcgt
    18541 ccgggtcaga aatcccgcgt tcgacgtgac gcccgcggaa ctcgtcaccg cgatcatctg
    18601 cgagaaggcc gtggtgcacg cgccgagccg cgagcgcctt cagcccctga tgtcgacctc
    18661 ggcatagggc ggtacgagct cgaacagctc ggcgacgtcc gcgttgcgca tgcggatgca
    18721 gccgtgcgag ccgggcgtgc ccatctctgc gctgtccggc gcaccatgga tgtagatcat
    18781 gcgccgcatc gtgtccacgt cgccaagccg gttccgccct acctcgcagc ccgagagcca
    18841 caggatgcgc gtcagcatcc agtcgcgccc ggggtacttg gcatgcagct cggggctcca
    18901 gacttcgccc gttggccggc gccgcacgaa taccgcgccg agcggctggc cggcgccgat
    18961 cttcgcgcgc acgatgtggc ggccacgcgg cgtgcagaag cttccgttct tctcgccgag
    19021 gccgttcttt gccgtggata ccgcgtagcg ccggccgtcc ggcaggcgca gctcctggcg
    19081 cgcgacgtcg atttcgaccc tcatcggcgc gcggcgaaga acgctttcag cgcggccccg
    19141 cattcttccg cgagcacgcc gccctgcact cttgcgtggt ggttgaaccg cgacatgaat
    19201 agttccgacg ccccggtctt cgggtccggc gccccataga ccacgcgagc gatgcgcgca
    19261 tggaacgccg cgcccacgca catgacgcag ggctcgagcg tgacgtacag cgtcgcgccc
    19321 gtcagccggt agttgcccgt cgccttcccg gcggcgcgca tggcttcgat ttcggcgtgc
    19381 gagctggggt cctgcgtgct gaccggccga ttccaccctt cgccgacgac cctgccgtcg
    19441 agcacgacca cggcgcccac gggaacttcg tcctcggtgc gcagggcgag ctcgagcgcc
    19501 cgccgcatga attgcgcgtc gctcatggga tcagtcgagc ggctcgagcg attcgggatc
    19561 ctcttcccag gtcatcgacg gacggggggt gcgcgcgatc cactcgtcga gcgactgcgc
    19621 atgctcgcgc tcctcggcag cgaattcgga cgccatgcgc cgcagctcgc tgtcccgcgc
    19681 ctcggcggcg acggaacggt agtacttcat cgccctgacc tcgttctgcc gtgcgtaacg
    19741 cagcgcgttg tagggctcca gcaggtaatc gaacgcctct tcgccgccga cctccggcgg
    19801 cgagcgccag cggtactccc aggaattgag ccgcggcagc tcaagcgtcc ccgcgcgctc
    19861 gaggatcgaa tcgcggtgca ggatcgaaaa ccggttcatg tcccggaaca gcgccgccgc
    19921 ggcgtcgttg cgatgcgcct ccatcatgtc cgccagctcc aggtaacggt ccgcggcctc
    19981 ctgctccatg gcgacggcgt gcgccaagaa ttcggtcagc gtcaagctca tcggcgcatg
    20041 ctacccgatg ccgcacgcct gcggccaggt gcagggtggg cgccggcacg cgcgggtcgc
    20101 gtaagatcgc tgctggcgca atacccatga tcgaacctct cctgctcgcc gtagcccgcg
    20161 tttcgacgtt ccggcagcaa cagccgctga ccaacgcgag cgggttcttc ttcgagcgcg
    20221 acgagcgcct gttcctggtg accagccggc acgtggtgag cgacgcgccg gcgcagcact
    20281 ttcccgaccg gctggaaatc gagctgcata ttgatgccgc ggacatggcg aagtcggcag
    20341 cagtatcgat ccccttgttc cgcgaaggcg cctccctgtg gcgccagggg gtggacgccg
    20401 ccggcgagat cgatgtcgcg gtcatcgaga tcgagcgcag caccctgccc gtgacggcgg
    20461 tgttccatgc tttctcgccc gcgcacctgt gcgatctgga gaccgacagg gtcgaggtcg
    20521 gcagctcgct gctgattgtg ggctttcccc tcggcttcaa ggacactctg caccacatgc
    20581 cggtggtgcg ccgcgcggct ctggcttccg ccttcggcct gcgcttcaat ggcgaaggct
    20641 atttcctcac ggacgcgcgc acgcatcggg gcacgagcgg cgcgccggtc gtgctgcgtg
    20701 cggccacgat ggcgccggca ctgggcaagc tgcaactgaa gctgctgggc gtgcattcgg
    20761 cgcgcgtcga cgcgggctcg cgcgaccgcg agatggacga ggcgctcggc ctcaacaccg
    20821 cctggtacgc cgacatcctg ctgaccctga ccgagggttg aaggcgcctc aggcgcgaca
    20881 ggtccgcagt tagagcgctg gcgggcgcgc gcgctcagcg cttgagcggg aagaactcca
    20941 ccggcgtcat cagtcgattc tcttgcgcgt ccagcgcgcc gagcttggcg ctctcctgcg
    21001 tatacgcgat ccactcggcg tcggcccaca tcgccgcgcg gcgcttctcg cggtcggcgg
    21061 cgttctcgta cacccagatg tggatgtact ggttgacgtt gccgctctcg gtgaccaggt
    21121 acgccagcgg ctggccaagg tgccgggtct gcggcgtcct gcccagcttg tcgtagagcg
    21181 cgaggtgcgc cttgatcgtt ccggggcggc aggtataggt acgtacgtcg agcagcatgc
    21241 gggtctctcc ttggttaacg gaaacgacgc ggcggacggc accagacgcc ggacaggtcg
    21301 gaatcgagcc acgacgcgcc gatcgcccat gcgccggcga tgacggcggt caggccgaac
    21361 caccaggcgc gctccagcgc gtgcgcgccg acggcgtcgt cgccgcccag catcgcatag
    21421 gtgccgatgg cgaggaagcc caggccgacg agcgtgccca ggcagcggag cgcgaggtgg
    21481 tttgtgtgga tgcgcacggt ggaagcatac tctgccatgg ccagagcact tgccgtcgcg
    21541 gcctgcgcgg gtctgctggg ctgcgcgatg cgcgcgcccg atccgcacga cgcagacgtc
    21601 ctctacctgc cgacgccgcc cgccgtggtg gaggcaatgc tcgatctggc cgacatccgt
    21661 ccaggcgacg tgctctatga cctcggggcc ggcgacggcc gcatcgtcat cgccgccgcg
    21721 cgccgctacg gcgtgcgtgc ggtaggcatt gagctcgata cgcgcaaggt ggcagaagcg
    21781 cgcgccaacg tggcgcgagc cggcctcggc gatcgcgtgg agatccgcca cggagatctc
    21841 ttcgacaccg acctcggcga ggcgagcgtc gtcaccctgt tcctgttccc ggagctcaat
    21901 gcgcgcctcg ccccgaagct gcgccgcgag ctgcagcccg gcacccgcgt ggtgtcgcac
    21961 cgcttcggac ttggcgactg gccgccggat cgccgtatcg aggcgcgggg ccacccgctg
    22021 ctgcgctgga cggttccgcc gcgctagcgg gacccgcccc cctcttcctc gatggatcgc
    22081 gccggccggt tgcggatgtc gtcgatgtgg cccgcgtcgt agctcttcga gcgcaggtcg
    22141 agcgtgcgct cctccaccca ggcctcgtcc acggtcgtcg cgcgacaata gagaaagtgc
    22201 agcatgagga gcatgcccca cagcacgagc ggccagaccg cccaccaggg cgcgcccgtc
    22261 gcgacgttga tgcccgtcag accggcgttg acagcgaggt agatgtacag gtggggcctg
    22321 aacgcgccca ccaggcgctc gcgcggcgta acgctcaacg cgcgtccggc tcggcaagcc
    22381 ccgcgtaggc acgggcgatc gcgatcaggc gaccgccgta agccacccac agcacgaccg
    22441 ccgcgaccag cagcgtcttg tcccccgccc ccaggctcgg gtgaaagccg aacaacgcga
    22501 aatagagcag cgctgcgagc ggtgcgctcg cgcagtagaa cgcggcccag gcgctacgcg
    22561 tcatgccgag cagcagcgcc gcgcacacct gcgtgaaggc gtagaccgag acgatgcgtg
    22621 cgtcgccgtg cggcaggctc tgcgacaacg ccgccagcga cacgagcgcg aaaaggacgc
    22681 cggtgatcac ggcatagaag cgcagctcgt actcgatctg cgcccgcgtc aggcgcgcca
    22741 cggaaatgag gtacggcgtg cgctcgtcgt cccagacgta cttgcgaaag tacttgtcca
    22801 tgcccgcggc gtcagccccc ggtcagtatc tttgaggtgc gctccttgag cgccgcgtcc
    22861 ggcgccacca gcgtgttggg cgcgtcctcc agcttgacgc gctccttgcc ctcgatcttg
    22921 gcgtcttctt ccggccactc gaaagtcatc agaggaaagc gccgcgccgg gtccccgaac
    22981 agaacgagcg cgacctgcag ccacaccgca agccccgcgg tcaccacggt aagcaggccg
    23041 aacacgagca cgctcttgat gatccagcgg tgcgtcaggc ccaccgtgga ggtggactgc
    23101 tcgttcatct ggaacgaccc gtaggcatag gtgacggcga agtacaggat cgtgagcgta
    23161 aatgggatga ggaagaaggt gatgccgaac agttccagcc aggccttctt gcgaaacgcg
    23221 aggttctcgc ggatcaggtc gacgcgcacg tgcgagttgt ggatgtaacc gtagcccagc
    23281 accagcgcaa acagcccggc gtggaaatgc cactccagct cctgcagcat ggtcgattcg
    23341 aaaatgcgac cgaaaaccga gacgaggaat acctgcatgc cgtgctgctt gccctcgtcg
    23401 tcgagccagt ggatcttgcg cccgaccacg tcgacgatgg tgaacagcac cataggaatt
    23461 accagccacg agccccagcg cccgacgaaa tccacgaagg cgcgcaggcg gttgctcagc
    23521 cgcagcgcca agggctcggc ttcgccggtg aatgtcgctt gcggcgaggc tgctctggct
    23581 gattcccgta ctgctgccat atccctcgcc tcctcagttg tttgcacgaa tgatccacaa
    23641 cgccacgtcc gggaacgcca gcacgatgat cacggccgtg agctgcagca gaacaaacgg
    23701 gatgatgccc ttgtagatgc tctggatact gacttctttg ggcgcgatgc ccttcaggta
    23761 gaacagcgca aaaccgaacg gcggcgtcag gaacgacgtc tgcaggttga ccgccatgag
    23821 gacgatgaac cagtaggtga tctccgcttt cggcacgtgc tcgccgaaat cgagcgtggc
    23881 gacgatcggc gcgaacaccg g
//
LOCUS       DBNHKE010000030         4324 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1115160, whole genome shotgun sequence.
ACCESSION   DBNHKE010000030 DBNHKE010000000
VERSION     DBNHKE010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1115160"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..799)
                     /locus_tag="AC16O9_01800"
     CDS             complement(50..799)
                     /locus_tag="AC16O9_01800"
                     /inference="COORDINATES: protein motif:HMM:NF025055.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HIR6515386.1"
                     /translation="MAALLCGVHALAWADEPTSLVNRAGEQRMLSQRMVKFYCQVGLG
                     VVPAQSQAGMLDSMQRFEANLDVLGPAAKRTPATAQAYGRLALAWRPVRAAAAKSATP
                     ESARALSRLAEDVLAAAEALTRAMEEASGQLISQAVNLAGRQRMLSQRVAKAYMLRSW
                     GVDSAAVREEMAAATSEFNGALALLMARPENTQEIRGELQDLALQWEWLQSAVSAEGA
                     TSYRLIVAEASEAILASADRITRLYDRIARR"
     gene            complement(832..1113)
                     /locus_tag="AC16O9_01805"
     CDS             complement(832..1113)
                     /locus_tag="AC16O9_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515387.1"
                     /translation="MLSLKFETAADELAYEQRRFRSSQIMVDRLVDEGRAGAERRRLV
                     DEFVGAGRRLKEEADTLAKSGSHREAVALMERANSQLNRALQVMGVPIF"
     gene            complement(1101..1694)
                     /locus_tag="AC16O9_01810"
     CDS             complement(1101..1694)
                     /locus_tag="AC16O9_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515388.1"
                     /translation="MLVEKWLVVGASLCTLVAGSALAQSPVRADAEEKRRLIEQKIRL
                     VETMINSPAARGAAGSGDPEAPALIERSREAARAAKAAIVDGRLDEASRAADEALKSA
                     ANASRRLSSRGAGLSESAQRQNFRDLREQIVGYRASVEEMAGDERLGSRARALLAHID
                     SPERGAGAMRTASSPKPIATRCGASPACVPVRRSCCR"
     gene            complement(1942..3270)
                     /locus_tag="AC16O9_01815"
                     /pseudo
     CDS             complement(1942..3270)
                     /locus_tag="AC16O9_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025017.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(3392..3772)
                     /locus_tag="AC16O9_01820"
     CDS             complement(3392..3772)
                     /locus_tag="AC16O9_01820"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6515389.1"
                     /translation="MHSEEFARAQQLVARGDHAGAEARFDRILAQQPGNVDALAGRGH
                     ARAFQKKYDGANADFRAVLARQPGHLGALIGLAHAHAWAGEHDAASCRDRWSRTSTAS
                     SSSSFSSADTSSSSAAGAGPATTG"
     gene            complement(3893..>4324)
                     /locus_tag="AC16O9_01825"
     CDS             complement(3893..>4324)
                     /locus_tag="AC16O9_01825"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HIR6515390.1"
                     /translation="PLYVRYAPGMQYGEHLDDPIMGAGGVMYRSDVAVTLFLSAPEAY
                     EGGELVIRGAFGETSVKLPAGDAVLYPAGTLHHVNAVTRGERLVAVTWVQSAVRDAAR
                     RELLYGLNLARESLLRDAPEAEATAQVNAAYLNLMRMWSEL"
BASE COUNT          663 a         1570 c         1508 g          583 t
ORIGIN      
        1 ccgagtctcc ggatcggcgc ggcgctcgac gaactggcgg ccttgctcgt cagcgccgtg
       61 cgatgcgatc gtagagtcgc gtgatacggt cggccgaggc gaggattgct tccgaagcct
      121 ccgccacgat caggcggtag gaagtcgcgc cctcggccga gaccgcgctc tgcagccact
      181 cccattgcaa cgcaaggtcc tgcagctcgc cgcggatttc ctgcgtgttc tccggccgcg
      241 ccatgagcag cgccagcgcg ccgttgaact cgctcgtcgc ggcggccatc tcctcgcgca
      301 ccgccgccga gtcgacaccc caggagcgca gcatgtaggc cttggccacg cgctgcgaca
      361 acatgcgctg gcgcccggca agattgacgg cctggctgat cagctggccg gaggcctcct
      421 ccatggcgcg cgtcagcgcc tccgcggcag cgagcacgtc ctcggcgagc cgcgagagcg
      481 cccgcgcgga ctcgggcgtc gccgacttcg ccgccgccgc gcggaccggc cgccacgcga
      541 gcgcgaggcg cccgtaggcc tgtgcggtgg cgggtgtgcg cttcgccgcg ggtccgagca
      601 catcgagatt cgcttcgaag cgctgcatcg aatccagcat gccggcctgc gactgcgcgg
      661 ggacgacgcc caggcccacc tggcagtaga acttcaccat gcgctgagac agcatgcgct
      721 gctcgccggc gcgattgacc agcgacgtcg gctcgtccgc ccaggcgagc gcgtggacgc
      781 cgcacagcag cgcggccatg gcgaggatgc gcaggatggg cttccttgcg ttcagaagat
      841 tggcacgccc atcacctgca gcgcccgatt gagttggctg ttggcgcgct ccatgagcgc
      901 gacggcctcg cggtggctgc ccgacttcgc cagcgtgtcc gcctcctcct tgagcctgcg
      961 gcccgcgccg acgaactcat cgaccagcct gcgccgctcg gcccccgcgc gcccttcgtc
     1021 caccagccgg tctaccatga tttgactgct gcggaagcgc cgttgctcgt aggcaagctc
     1081 gtcggccgcg gtctcgaatt tcagcgacag cacgatctcc tgaccggcac gcaggctggc
     1141 gacgccccgc accgtgtcgc gataggtttc ggcgagcttg cggttcgcat cgccccagcg
     1201 ccccgctccg gcgagtcgat gtgcgcgagg agcgcgcgcg cgcgcgaacc caggcgctcg
     1261 tcgcccgcca tctcctccac cgacgcccgg taccccacga tctgctcgcg caggtcgcgg
     1321 aaattctggc gctgcgcgct ctccgaaagc ccggcgccgc ggctcgagag gcgccgcgac
     1381 gcgttcgccg ccgatttcag cgcctcgtcg gcggcccggc ttgcctcgtc gaggcggccg
     1441 tcgacgatcg cggccttggc cgcccgcgcc gcttcccggc tgcgctcgat caaggccggc
     1501 gcctcgggat ctcccgaccc ggccgcgccg cgcgcggccg gcgaattgat catggtttcg
     1561 acgagcctga tcttctgctc gatcagccgg cgcttctctt cggcatcggc gcggaccggg
     1621 ctctgcgcca gcgcactccc cgcgacgagc gtgcagagcg atgcgccgac gacaagccac
     1681 ttctcgacca acatcgcttc cccagtcccc ctgccggctt tccgaatacc ggtccattat
     1741 gtcccagaac cgcggacgca tggcagggtc ggccaccgtt cccgccgcgg ccgaagcacg
     1801 cagccagcgc cgcaagacgg cgacaagttg ctcgcgcgcc gcgcgcccgg cccgggggtc
     1861 gtgcatgccc gcgtccacgg acttctcggg aaagccatgc aaggcgtgcg gcgtgaccgg
     1921 ccgccgcctc gtcgcgccgg ctcagaagtg cacgccgaag ttcaggaaga cgggctggat
     1981 gccgatcaag gagagcgttc cgagaccgag cccgccgatc atgagcaccg cgggaacgtg
     2041 gacggccacg cgcagaaagc ggctttccca cggcaggtgg tgcatcagcg ggtgcagcag
     2101 gtacgcgagc gccagtccga ggaacgacac ctggaacgtg cgcagcgcga cgagcacgaa
     2161 gaacgcgccc agcgccacct cgatggcctg gaccgcgggg tgatccggat ggctgcgcac
     2221 gagaaagcgc cgcacggcgg gaaacaggct ctcgagcggc gcgaggcccg aggcgagcgt
     2281 gccggcgagg gacgcgcgcg tgcggtcggc ggtcacgtga aacaccgcct ggcgcgtgag
     2341 cgagaagcac accacgccga tgcacgacac gggccccagc gccccgtaca cggcgacgct
     2401 gtggctgagg aactcgagca gccggcgcgg tgtgcgccac atttcgacga gaaacgagag
     2461 caccggggcg gtcagcgcga gcaccgtgat cgcgtagaag tcgaagccgg tcacgctgcc
     2521 gaatcgcccg tcgagcgtca tcaccgggaa gacgagctcg cgcgtgccca gggcgagggt
     2581 cagcaggtgc tcgcgcccga acagcgccgg gagcgccacg ttcacgtcga gcacgaagac
     2641 gaagaaaaag agcgagagcg gcaatcccag ggtcgggaac aggatgtcga tcttctcgac
     2701 ccagggcacg tgcggcgagc cgagcaggta gcgcatcttg cggtagagga actcgcaggt
     2761 gccgcgcgtc cacttcatgt ggcgcacgcg gaacgcgcgc atgtcgggcg ggaagttctc
     2821 gaaacagacg acgtcgtcgg cgaacacgcc gtgccagccg tcttcgcgga tgcgcagcgc
     2881 gtaggcgaga tcttcgctga cgaggtgggg aaatccgccc acctcttccc acggctggcg
     2941 gcgcaggagg gcgccgtgcc cgagcagcat cacgaagccg aagtggttgc gcagcgggtg
     3001 gtaccagcgc cagtgcacgt cgatgcccac gccgagcgcg cgcgccaggg ggctcgcgtc
     3061 gcccgggttg ccgcggtgaa tcgcctgcac gaaaccgcag cgctcgtcgg ccagcatgcg
     3121 cggcacgaga cgcgacagga agtcgggcgg caggagctcg tccgcgtcca ccagcgcgaa
     3181 gaagggctcg cgcgtcgcgg cggtcgccag gccgtggttg atgttgccgg ccttgtagcc
     3241 cctgcggtcg gcgcgccgca cgacctggac gcgagcggga gccgccgctc gcggtcatcg
     3301 ccgaccccgg tgcgcgaaga aaagtaggtc ggctcgaatc gccacgcgcc ggtgagcgcc
     3361 agccccagcc cggcgtacca cagggactcc gtcaccccgt cgtcgcgggg cccgcgccag
     3421 ccgccgagct tgacgaggtg tccgcgctcg agaaagaaga gctgctcgac gcggtagatg
     3481 tcctgctcca ccggtcccgg cagctcgcgg cgtcgtgctc gcctgcccac gcgtgcgcgt
     3541 gggcgaggcc gatcagggcg ccgaggtgcc cgggctgcct cgcgagtacc gcccggaaat
     3601 cggcgttcgc gccgtcgtat ttcttctgga acgcccgcgc gtggccgcgg cccgccaggg
     3661 catccacatt tcccggctgc tgcgcaagga tgcggtcgaa gcgtgcctcc gcgcccgcgt
     3721 gatcgccgcg cgcgacgagc tgctgcgctc gcgcgaactc ttcgctgtgc acgccgctgg
     3781 cggcctgcgc gcgcgactcg gcaggaatgg cgcccgcgcc gacgacgcag gctgtgacct
     3841 cgggctcagc gagcgcgggc gagcgatcgg gcgcgcagca ggcgcgcgcc cctcacagct
     3901 ccgaccacat gcgcatgaga ttcagatacg cggcgttcac ctgggccgtc gcctcggcct
     3961 ccggtgcgtc ccgcagcagg ctctcgcgcg cgaggttcag gccgtagagc agctcgcgcc
     4021 gcgcggcatc gcgcaccgcg ctctgcaccc aggtcacggc gacgaggcgc tcgccgcgcg
     4081 tcaccgcgtt cacgtggtgc agcgtgcccg ccgggtagag caccgcgtcg cccgccggca
     4141 gcttgacgga cgtctcgccg aatgcgccgc ggatcacgag ctcgccaccc tcgtacgcct
     4201 cgggcgcgct gagaaagagc gtcaccgcga cgtccgagcg gtacatgacg ccgcccgcgc
     4261 ccatgatcgg gtcgtcgagg tgctcgccgt actgcatgcc gggcgcatag cgcacgtaaa
     4321 gcgg
//
LOCUS       DBNHKE010000031        18168 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_163082, whole genome shotgun sequence.
ACCESSION   DBNHKE010000031 DBNHKE010000000
VERSION     DBNHKE010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 18168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18168
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_163082"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..3511
                     /locus_tag="AC16O9_01830"
     CDS             35..3511
                     /locus_tag="AC16O9_01830"
                     /inference="COORDINATES: protein motif:HMM:NF017183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR6515391.1"
                     /translation="MRIARDVEHLYLEVDSAGRYVTLENWFGQGAAGAVAFADGTVWD
                     GASLKAIVDAPTEGDDFIVGTDAADIIATLGGFDVVFAGDGDDHISGGTGSDTLDGED
                     GDDVLLGEAGYDLLSGGPGNDLLDGGIGDDELRGGLGGDTYVFKLGYGSDLIVENDPN
                     AGNADSIVFDALPSDVTVARAVSDVRFTLAGGADQLTVQNWFNGSAMQIEAVRFADGT
                     VWDAAMVTALATGQPLKAPGTTASGTVLAESSTETAPRGDTVTPAAAMLGLVADLTPA
                     ADFFRGSGGDDVLDGLGGDDTLLGGPGNDTLRGGDGDDYLVGEAGNDTLDGGIGRDIL
                     EGGAGDDRYVFGAGYGQDWALDVGGSDEVIFDSTIAPAAVVFTRDLSNLYVGAGADRL
                     TLVDWFYRADTRIEMFRFADGSSLDEAAVRLALQVSAASSADDTIFASDRGETLLGLA
                     GEDTLYGEGGDDVLDGGTGSDYMLGGKGNDTYYVDNRLDRVTEAPDEGTDTVFASASH
                     ALAPHVENLVLTGTAPLSATGNDGNNVIDGNAGANTLRGGRGDDWLRGGAGNDAYLYD
                     QGDGNDVVDDVDATPGNADEVRFGQGITSAAVRVRRDDQDVRLVVQGGGEVRLRSWFD
                     TAARIEAVRFADGTAWDAAMIEFLASQPVNDPPVLSRPIDDVSTLEDEAFVHVLQPGT
                     FVDPDTADTLTFAASRADGSELPAWLAFDPESAAFSGLPGNADVGEYAIRVTATDGAS
                     QSASDEFVLTVINVNDAPELALPVADQSVSVGSGFALSLEAGMFTDVDPGDALTYAAT
                     RADGSALPEWLVFDAASRSFSGTPAASDAGLLDVRVTATDLAGVSASDEFRITVVGEE
                     PSGKHLIGTSRGDVLVGTPYDDTIEGRQGGDLLIGLAGSDVIEGGKDDDLILGGNGDD
                     AYLFARGDGDDLIIETGGHDVLRFGAGISRHDVAVMRDRGDLVLRLKGYGGSVTVRNW
                     FDSDSRRVERVEFADGSSWGEAELWWQAKRHTVGSWSDDRRNGEWNDEKRRHQWHDRG
                     GDGHRGRSSGEDYGARDARALIARRLEAAARYDFGAVADYLRQQAEQRAEARTWARVA
                     GQWAEVQSALGCLEQSHQEASHGAQDGRDRDHLLAHGDTGWGHASSTGRRHAYAGLQS
                     FTGLGEGFRNLG"
     gene            3508..4923
                     /locus_tag="AC16O9_01835"
     CDS             3508..4923
                     /locus_tag="AC16O9_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HIR6515392.1"
                     /translation="MSKQAEDGDPPSLERALLSLGARIRADAREFRPAIVRLQESPPS
                     PLGRRVLWAALIFVASLLAWSVFGRLDVVAVAEGRLVPDSYLKIVQPADAGVVKEILV
                     REGQTVSAGQVLMRMDAALSDSDLKALTAEVGGKRLALRRIDAELVGSPLTRRADEPA
                     AQFTHVLAQYHANRQAYETALAQERATLDKARFELRAAEEVRDKLRSVLPHYRSQEAA
                     YEKLTRDGFAGRLMYTDKQRERIEKEQDLRAQEAAIASASATIAQQKRRIAQLTADYR
                     RRLQTERVEVASQLERLTLDLAKQEHRHNYLELRAPAEGQVKDLATHTIGAVVSPGTI
                     LMTLVPKDEALRAEVWVQNDDIGFVREQQPVKLKLAAFTFQKYGMLQGTVAHLSADAS
                     EQAGAGPETASASTAPARGALAYRTLVDLKTQTLEADGVTHRLSPGMQVTAEIHLGTR
                     TVLEYLLSPVRQGFHEAARER"
     gene            complement(4997..6280)
                     /locus_tag="AC16O9_01840"
     CDS             complement(4997..6280)
                     /locus_tag="AC16O9_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6515393.1"
                     /translation="MTIALILAGLVLLLFTGLPIFAGLTLFGGGLLIATQGGLGSVTE
                     VIFGELNRYLLVAIPLFAFMAHVMIRSRIVDDLYGTAYTLTRHLPGGVGLATIAACTI
                     FAAISGSSVATALTIGSVAIPQMIRFGYTPRAAYGLVAAGGTLGILIPPSGPMVLYGV
                     VTDSSIGGLFMAGLIPGLLMAAIFAVYTMLAAALGARAVRREPRAGFREALSALRKSV
                     WALSLPALVLGGMYAGVFTATEAAGAGATLALLIGAFVYRTIGWREIWDSAMDASRTS
                     AMLFMILAGATVLGFVLTKMRVPHEIVQVVVQQDIGVLGFLLAMMALLFVLGMFLETI
                     AIILITTPVILPAMTHLGIHPIWYGVLLMINLELALITPPVGMNLFTIKAVTKAPIGE
                     IIRGALPYVVLIFVCLGIVMAWPQLALWLPGTMFK"
     gene            complement(6277..6822)
                     /locus_tag="AC16O9_01845"
     CDS             complement(6277..6822)
                     /locus_tag="AC16O9_01845"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6515394.1"
                     /translation="MTRAAAAAARAARVIGALADGAAALSAAGVLVSLALIAWAVVMR
                     YVFNRAPVWVDDIVGFMLVGIVMLAAAQVLRRGEHIGVDLLTARLSPRARRWADAWAA
                     LGALAVALILIVNGWRTAMFAKQLGIVAEGRVEIPVFWLQLLLPLGGLLLALAALEAL
                     ARLAAGLPPVIKPQEATDKPE"
     gene            complement(6833..7819)
                     /gene="dctP"
                     /locus_tag="AC16O9_01850"
     CDS             complement(6833..7819)
                     /gene="dctP"
                     /locus_tag="AC16O9_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR6515395.1"
                     /translation="MNLKAKFGLAAFATLVAAGAQAADITMRLSHQFPPAHHSAKNME
                     QFAADVKANTNGKVEVQIFGAAQLYKPNQHHAAVAGGKIESAIVLSFQWGGTIPEMNV
                     TIIPYFMSSVDKMKKFPGSEAARMLDAKMLEKGVKNIAWLVDANDGIFTSAKAPLIKP
                     EDFKGVKIRGLSKLWDEGLVAMGAAPSAMPGSEVYQALQTGVIDAGFTGVQAAHSRRF
                     YEVQKFGVASNIILAYDNLIVNPEWWEKLPADVKKGILDALEKAEARVMPKDDAIPPE
                     HLQQLRDKGMNVTELSAGQKKVMADAMQPAVIKAFSASSPDAPRLLELIRGL"
     gene            complement(7816..8127)
                     /locus_tag="AC16O9_01855"
     CDS             complement(7816..8127)
                     /locus_tag="AC16O9_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515396.1"
                     /translation="MAAENEILLTVFLKHQQDKNLGEINAKLDAAGFWKQFPPQGIEV
                     ESWYVMMGIGQVVTLRVPASRLRDVNLTLEKFAWGAFNTEFYATYDFRPVWQKLKEGT
                     K"
     gene            complement(8232..9443)
                     /locus_tag="AC16O9_01860"
     CDS             complement(8232..9443)
                     /locus_tag="AC16O9_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6515397.1"
                     /translation="MPSADAIRDFQLTRVPADFLDDPYPWYAALRERDPVHALEGGGV
                     FLARYEDALAVYRAPQASSDKKAEFGPKLGDSPLYEHHTTSLVFNDPPLHTKVRRILM
                     GAVNQKAIARMEPNVARLVDTLLEEMAAKREVNFIDDFAAQIPIEVIGNLLDIPREER
                     GPLRGWSVAILSGLEPKLTPEMLEAGNRAVREFIDLLKRLVAARRKHPGDYESDVLTR
                     LIQGEKDGEQLTEKELYHQCIFLLNAGHETTTNLIGNGVWALLNNPGELARLRADPSL
                     VPSAIEEMLRYDGPIQLNNRRLTAPLALAAGTLPEGTLITIGIGAANRDPAQFPEPER
                     FDVGRKPNRHLAFGQGDHVCVGMNVARMEGRIALARLLARFGRIELAGAPERDRRVRF
                     RGFRKLPLRLS"
     gene            9461..9994
                     /locus_tag="AC16O9_01865"
     CDS             9461..9994
                     /locus_tag="AC16O9_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6515398.1"
                     /translation="MQRVPNTEFPPELKKRLEELWGSPPNLYRALANHAPLAAAWTEF
                     ARAIRYESRTPRTLRELVILRGAQLMRSEYEWAQHLKMARKAGVREAQIAALALWRDS
                     GEFDATEKAALALAEAVTRADVTDEVYAAVSKHFDHATYVELALTAAFYAMVGRMLDA
                     MRVQLEPEFRDFQPKLP"
     gene            10045..10710
                     /locus_tag="AC16O9_01870"
     CDS             10045..10710
                     /locus_tag="AC16O9_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR6515399.1"
                     /translation="MAAFVTVLICANLIGPAKISQVELPLVGTLVFGAGVLFFPISYV
                     FGDILTEVYGYARARRVIWAGFGGLAFASFMAYVVVALPPAPFWKNQQAYEIAFGQAW
                     RIAGASMIAYFCGEFVNSFVLAKMKIMTAGRWLWTRTIGSTIAGEAVDSALFYPLAFY
                     GTGIIPDDKLLAVMAFQFVAKVLVEVAFTPLTYKIVGLLKRAEREDFYDRNTDFTPFS
                     LRT"
     gene            10850..13321
                     /locus_tag="AC16O9_01875"
     CDS             10850..13321
                     /locus_tag="AC16O9_01875"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HIR6515400.1"
                     /translation="MVAERLPVPRLGQSAPLRVLLIEDNKADAMVAQAAIEKAAIGRA
                     EVLRAESIARGLGIAGAAEVHLVLLDLNLPDSRGLATLERMRAAVRCPVIVVTAEERP
                     GLDEEALEMGAFEILHKGKLGANAIARVLRLAEGQRKVQASLESAEQRYRKLVEIAPD
                     AIFVHADWRIALVNPAMLRLFGATRPDELLGREVLELIAPAARGVARERIRGLYESAQ
                     SVQLREVEYVRRDGTPFSSEVTAVSFTFDGRPAVQVVARDITERKSAELALRRSEERF
                     RGLTELSSDWYWEQDKALRFTYVSPGFEERSKHDPALVLGKRRWDFDNVMPESGTWDD
                     HRKVLEARRPFRDFEQITVRPDGARVHISSSGEPVYDESGAFTGYRGVSSNISVRKAA
                     EARLEQLAQFDSVTGLANRNLLHERLEHAVVQSRRRGRGAGVLYVNLDRFKLVNDTHG
                     HHVGDDLLRHVGRRLQDCVRRDDTVSRLGSDEYAVVIADLARADDAALVAQKLLDALA
                     APFDLGGRETFITASIGVATFPTDGESAEALLKCADIAMGRAKESARNTFCFYTAELN
                     ARAASKLQLNSDLRRALERGEFLLHYQPKVRLASGQMIGMEALLRWQHAQRGMVAPLE
                     FIPALEESGQIVPVGDWVIAQACRQLAEWLRAGLQSAPVAVNLSARQFRRRDLDRVIK
                     DMLAEHGVAPGMLELEITESSLMDDPKDAIRQLHALREAGLRISVDDFGTGYSSLSYL
                     TRLPVSTLKIDRAFVNAAIREPSSAAIVRMVIDMALRLRFDVVAEGIESEEHVAFLRE
                     HGCEQGQGYHFGRPIPAAEMARRLK"
     gene            complement(13432..14865)
                     /gene="dacB"
                     /locus_tag="AC16O9_01880"
     CDS             complement(13432..14865)
                     /gene="dacB"
                     /locus_tag="AC16O9_01880"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR6515401.1"
                     /translation="MPRFCRLALAAVLLLAPLPSTAAASGLPKPVAEALRAASVPLRA
                     VAVVVQEVGAARPAVSMNAEAAMNPASAMKLFTTLAALELLGPAYHWKTEVYAAGPLR
                     DGVLEGDLVLKGYGDPKLDYESFWMLLRTLRGKGLREIRGDVLIDRRFFAPLAGDPGD
                     FDGEALRPYNVLPDALLVHYKALRFAFVPLADGAGVRIYAEPRPPALEVKNSLRLSSG
                     PCFEGRAFRTLLQASFEPGPPPRAAFEGRYPIACGEKDLNVAFLPPDEHVGGVLRELW
                     SEMGGAWSGRAREGAVPEAAQLLHTHESAPLAEIVRDVNKFSNNVMARQLFLTLATAG
                     YEPPAQLHQAQQVLRHWLNLKGIAAPELSIENGSGLSRTDRASAATLAALLQAAWKSA
                     VMPEYIASLPLAALDGTMRKRLHGEPVAGQAHMKTGLLSDARALAGYLLDAKGRRQIV
                     VMLMNHPNAPEAEAANDALLRWVYEAK"
     gene            15027..15320
                     /gene="groES"
                     /locus_tag="AC16O9_01885"
     CDS             15027..15320
                     /gene="groES"
                     /locus_tag="AC16O9_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6515402.1"
                     /translation="MKIRPLHDRVIVKRIDEERKTASGIVIPDTAAEKPDQGEVLAVG
                     KGKKTDDGKLIQLDVKVGDKVLFGKYSGQTVKVKGDELLVMREEDIMGVLEGA"
     gene            15361..17016
                     /gene="groL"
                     /locus_tag="AC16O9_01890"
     CDS             15361..17016
                     /gene="groL"
                     /locus_tag="AC16O9_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6515403.1"
                     /translation="MPAKEVRFHDSARTRMVHGMNILADAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVRE
                     GMKFVASGMNPMDLKRGIDKAVIAVTDELKKISQPCKTSKEIAQVGSISANSDSDIGK
                     IIADAMDKVGKEGVITVEDGKSLQNELEVVEGMQFDRGYLSPYFINNPEKQIAVLDDP
                     YLLLHDKKISSIRELLPILEQVAKSGKPLVIIAEEVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEELGLKLENATLKDLGKAKKIEAGKENTTIID
                     GAGVKKDIEGRVKQIRVQVEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVAFLRARKALEGKLKGENHDQDAGIKIVMRALEE
                     PTRQIVENSGAEPSVVLNKIMEGKGVNYGFNAQTDEYGDLVDMGVIDPTKVARTALQN
                     ASSVAGLMLTTDAMVAELVEEKKGGGHAHPDMGGMGGMGGMDM"
     gene            17124..17199
                     /locus_tag="AC16O9_01895"
     tRNA            17124..17199
                     /locus_tag="AC16O9_01895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17157..17159,aa:Phe,seq:gaa)
     gene            17345..>18168
                     /gene="hemA"
                     /locus_tag="AC16O9_01900"
     CDS             17345..>18168
                     /gene="hemA"
                     /locus_tag="AC16O9_01900"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6515404.1"
                     /translation="MTLFALGLSHTTAPLPVRERVVFHVERLYDALGELTRGQRVAEA
                     AILSTCNRTELYLSAGEPQTAAEWFARYHSLQPEELRPYLYTLPQDQAVRHTFRVAAG
                     LDSMVFGEPQILGQLKDAVRTAEAAGTLGTVLHKLFQRTFAVAKEVRSTTPIGASSVS
                     MAAAAVKLAARIFPSLREQKVLFIGAGEMIELCATHFAAQAPARIAVANRTLERAQAL
                     AHRFAGHAVELRALAEHLQDYDIVISCTASSLPILGKGLVERALRARKRRPMFMVDL"
BASE COUNT         3058 a         5999 c         6326 g         2785 t
ORIGIN      
        1 gctgctcttc ggcgccggca tcgcgccggc gcaggtgcgc atcgcgcgcg acgtcgagca
       61 cctctacctg gaggtggact cggccggccg gtacgtgacg ctggagaact ggttcgggca
      121 aggtgcggcg ggtgccgtgg cgtttgccga cggcacggtg tgggacggcg cctccctcaa
      181 ggcgatagtg gatgcgccga ccgaaggcga cgacttcatc gtcgggactg acgcggcgga
      241 catcattgcg acgctgggtg gtttcgacgt cgtcttcgcc ggcgatggcg acgaccatat
      301 ttcgggcggc accggctcgg acacgctgga cggcgaggat ggcgacgacg tcctcctggg
      361 cgaagccggc tacgacctgt tgagcggtgg cccgggcaac gacctgctcg acggcggcat
      421 cggcgatgac gagctgcgcg gtggtctcgg cggcgacacg tacgtgttca agctcggcta
      481 cgggtcggac ctcatcgtcg agaacgatcc gaatgccggc aacgcggatt cgatcgtctt
      541 cgacgccttg ccttcggacg tgacggtcgc cagggcggtc tcggacgtgc ggttcacgct
      601 tgcgggcggc gctgaccagc tgacagtgca gaactggttc aacggcagcg cgatgcagat
      661 cgaggcggtg cgctttgccg acggaacagt atgggacgcg gcaatggtga ccgcgctcgc
      721 gacaggccag ccgctgaaag ctccagggac aaccgccagc ggcactgttc ttgccgaatc
      781 gtctacggaa acggccccgc ggggcgacac cgtgactcct gcagctgcaa tgctcggcct
      841 tgtcgccgac ttgaccccgg cggccgattt cttccgcggc tcgggcggcg atgacgtgct
      901 ggacgggctc ggcggcgacg acacgctatt gggcgggccc ggcaacgaca cgctgcgggg
      961 aggcgatggc gacgattacc tcgttggtga ggccggcaac gacacgctgg acggcggtat
     1021 cgggcgagac attctcgaag ggggtgctgg cgacgatcga tacgtgttcg gtgcagggta
     1081 cgggcaggat tgggcgctcg acgtcggcgg ttcggatgaa gtgatcttcg actcgaccat
     1141 cgcgcctgcc gcggtcgtgt tcacgcgcga tctctccaac ctgtacgtag gcgcgggcgc
     1201 ggatcgcctg acgctcgtcg actggttcta tcgcgcggac acccgcatcg agatgttccg
     1261 gttcgccgac ggcagctcgc tcgacgaggc cgcggtgcgc cttgcgttgc aggtgtcggc
     1321 ggcgagtagt gcggacgaca ccatcttcgc ttcagaccgg ggcgagaccc tcctcgggct
     1381 cgcgggcgag gacaccctgt acggcgaggg cggcgacgat gtcctcgacg gcggcaccgg
     1441 ctccgactac atgctcggcg gcaaagggaa cgacacctac tacgtggaca accggctcga
     1501 ccgggtcacc gaagcccccg acgaaggcac cgacacggtg ttcgcctctg cgagccacgc
     1561 cctggcgccc cacgtcgaga acctggtgct caccgggacg gcgccgctga gcgcgaccgg
     1621 aaacgacggc aacaacgtga tcgacggcaa tgcgggtgcg aacaccttgc gcgggggtcg
     1681 cggcgacgac tggctgcgcg gcggtgcggg gaacgatgcg tacttgtacg accagggcga
     1741 cggcaacgac gtcgtcgatg acgtcgacgc gacgcctgga aacgccgacg aagtgcgctt
     1801 cggccagggg atcacgagcg ctgcggttcg cgtaagacgc gacgatcagg acgtacggct
     1861 cgtggtccag ggcggcggcg aggtgcgact gcgcagctgg ttcgataccg ccgcgcgcat
     1921 cgaggccgtg cgcttcgccg acggcaccgc gtgggatgcg gcaatgatcg agttcctcgc
     1981 cagccagccg gtgaacgatc cgccggttct gtcgcgcccg atcgacgacg tttcgacgct
     2041 ggaggacgag gctttcgtgc acgtgctcca gccggggacg ttcgtggacc ccgacaccgc
     2101 ggacacgctg acgttcgctg cgtcgcgcgc ggacgggtcc gagctgccgg cctggcttgc
     2161 ctttgacccg gagagcgcgg cgttctccgg cctgcccggg aacgctgatg tgggtgagta
     2221 cgcgatccgg gtgacggcga cggacggcgc aagccagtcg gcgagcgacg agttcgtgct
     2281 gaccgtgatc aacgtcaacg acgcgcccga gctcgcgctt ccggtggccg atcagtccgt
     2341 ctcggtggga tccggcttcg cgctgtcgct ggaggcaggc atgttcaccg acgttgatcc
     2401 cggtgacgcg cttacctatg cggcaacgcg ggctgacggc tcggcgctgc cggagtggct
     2461 ggtcttcgat gcggcatcga ggagcttctc gggcacgccg gccgcctccg atgccggact
     2521 cttggacgtg cgcgtcacgg cgaccgacct cgcgggcgta tcggcgagcg acgaattccg
     2581 catcactgtg gtgggcgagg aaccctccgg aaagcacctc atcggcacga gccgtggcga
     2641 tgtgctggtc ggcacgccat acgacgatac gatcgagggc cgccagggcg gcgacctgtt
     2701 gatcggactc gcgggcagcg acgtcatcga gggtggcaag gacgacgacc tgatccttgg
     2761 cggaaatggc gatgacgcct atctcttcgc cagaggcgat ggcgatgacc tgatcatcga
     2821 gacgggagga cacgatgtgc tgcgcttcgg cgcgggtatc tcccggcacg atgtggcggt
     2881 gatgcgcgac cggggcgacc tggttctcag gttgaagggg tatgggggca gcgtcaccgt
     2941 ccgcaactgg ttcgactcgg attccaggcg cgtcgagcgc gtggagtttg ccgatggcag
     3001 ctcgtggggc gaggctgagc tctggtggca ggcgaagcga cataccgtgg gcagctggtc
     3061 ggacgaccgg cgcaatgggg agtggaacga cgagaaacgt cggcatcaat ggcatgaccg
     3121 gggcggcgac ggccatcgcg ggagatcgag cggcgaggac tatggtgctc gcgacgcgcg
     3181 cgcgttgatt gctcgccgcc tcgaggccgc tgcgcgctac gacttcggcg ccgtcgctga
     3241 ctacctccgg cagcaggcag agcaacgcgc ggaggcgagg acctgggcgc gcgtagccgg
     3301 gcagtgggcc gaggtccaga gcgccctcgg ctgcctggag cagagtcacc aagaagcgag
     3361 ccacggggcg caggacggtc gcgatcgaga ccatttgctc gcgcacggcg acaccgggtg
     3421 gggccacgca tcctcgaccg gccgtcgtca cgcgtacgcg ggcttgcagt cgttcaccgg
     3481 gcttggtgaa ggcttcagga atctcggatg agcaagcaag ccgaagacgg cgatccgcct
     3541 tcgctcgaaa gggcgctgct gagtcttggc gcgcgcatcc gtgccgatgc gcgcgagttc
     3601 cgtccggcaa tcgtccgcct gcaggagtct ccgccctcgc cgctcgggcg ccgggtgctt
     3661 tgggcggcgt tgatctttgt tgcatcgctg cttgcatggt ccgttttcgg caggctggat
     3721 gtggtcgctg tcgccgaggg tcggctcgtg ccggacagct acctgaagat cgttcagccg
     3781 gcggacgcgg gcgtcgtcaa ggagattctc gttcgggagg gccagaccgt cagcgcgggc
     3841 caggtgctga tgcgcatgga cgcggcgcta tccgattcgg acctcaaggc actcaccgcc
     3901 gaggtcggcg gcaagcgact cgcgctacgc cgcatcgatg cggagcttgt tggctcgccc
     3961 ctgacgcgca gggcggacga acccgcggcg cagttcaccc acgtactggc ccagtatcac
     4021 gcgaaccgcc aagcttacga aacagccctc gcccaggaac gcgcgactct cgacaaggcg
     4081 cgcttcgagc tccgggccgc tgaggaagtc cgtgacaagc tgcgaagcgt gctaccgcac
     4141 taccgcagcc aggaggccgc gtacgagaag ctcacgcgtg acggctttgc cggacgcctg
     4201 atgtataccg acaagcagcg cgagcgcatc gaaaaagagc aggatttgcg tgcgcaggag
     4261 gcggcgattg catcggccag cgccaccatc gcgcagcaga aaaggcgaat cgcgcagctc
     4321 acggccgact atcggcggcg gctgcagacc gagcgggtgg aagtcgcctc ccagctcgag
     4381 cgcctgacgc tggatctcgc gaagcaggag caccgccaca actatctcga actgcgcgcg
     4441 cccgccgagg gtcaggtcaa ggatctggcg acgcacacga tcggcgccgt ggtcagcccg
     4501 ggcaccatcc tgatgaccct ggtaccgaag gacgaagcgc tgcgcgccga ggtctgggtg
     4561 cagaacgacg acatcggctt cgtgcgcgag cagcaaccgg tgaaactcaa gctcgccgcg
     4621 ttcacgttcc agaagtacgg catgctgcaa ggcacggtcg cgcacctgag cgcggacgct
     4681 tccgagcagg cgggggcggg gccggaaacg gcgagcgcct caacggcccc tgcgcgcggc
     4741 gccctggcgt accggacgct cgtcgatctg aagactcaga cgctggaggc cgatggcgtc
     4801 acccatcgac tctctccggg catgcaggtc actgccgaga tccacctcgg cacgcgcacg
     4861 gtgctcgagt atctcctttc ccccgtacgc cagggcttcc acgaagcggc gcgcgagcgc
     4921 taaaacggaa aatggggaca gacctcattt ttctttcttg aggacgaagt gcagcaaaga
     4981 aaaatgaggt ctgtccctat ttaaacatcg tccccggcag ccacagcgcc agctgcggcc
     5041 aggccatgac gatgcccaaa cacacgaata tcaagacgac gtaaggcaac gctcctctaa
     5101 taatctcccc gatcggcgcc ttggtcaccg ccttgatggt gaagaggttc atgcccaccg
     5161 gcggtgtgat cagagccagc tccaaattga tcatcagcag caccccatac cagatggggt
     5221 gaatccccaa atgcgtcatc gccggcagga tcaccggcgt ggtgatcagg atgatggcga
     5281 tcgtttccag gaacatgccc aggacgaaca gcagcgccat catcgccagc aggaacccga
     5341 gcacgccgat gtcctgctgc accaccacct gtacgatctc gtgcggcacg cgcatcttgg
     5401 tcagcacgaa cccgagcacg gtggcgccgg cgaggatcat gaagagcatg gcggaggtgc
     5461 ggctcgcgtc catcgccgag tcccagatct cgcgccagcc gatcgtgcga tagacgaagg
     5521 caccgatcaa gagcgcgagc gtcgcgcccg cgcccgccgc ttcggtggcg gtaaacacgc
     5581 ccgcgtacat gccgcccagg acgagcgccg ggagcgagag cgcccatacc gacttgcgca
     5641 gcgcgctgag cgcctcgcga aagccggcgc ggggctcgcg gcgcacggcg cgcgccccga
     5701 gcgctgcggc gagcatcgtg tacacggcga agatcgccgc catcagcagg cccgggatca
     5761 gccccgccat gaacaggccg ccgatcgagg aatcggtgac cacgccgtag agcaccatcg
     5821 gccccgaggg cgggatgagg atgccgagcg tgccgcccgc ggcgaccagg ccgtaggccg
     5881 cgcgcggcgt gtagccgaag cggatcatct gcgggatcgc caccgagccg atggtgagcg
     5941 cggtggcgac gctcgagccc gagatcgcgg cgaagatggt gcaggcggcg atggtggcca
     6001 gccccacgcc gcccggcagg tggcgcgtga gggtgtaggc ggtgccgtag aggtcgtcca
     6061 cgatcctgct gcggatcatg acgtgcgcca tgaacgcaaa gagcgggatc gcgacgagga
     6121 ggtaccggtt cagctcgccg aagatcacct cggtgaccga gcccaggccc ccctgcgtgg
     6181 cgatgagcag gccgcccccg aagagcgtga gcccggcgaa gatcggcagg cccgtgaaga
     6241 gcagcagcac gagccccgcg aggatgagcg cgatcgtcac tcgggcttgt ccgtggcctc
     6301 ttggggcttg atcaccggcg gcaggcccgc ggcgaggcgc gcgagcgctt cgagcgcggc
     6361 gagcgcgagc agcagcccgc cgagcggcaa gaggagctgc agccagaaca ccgggatctc
     6421 gacgcgcccc tcggcgacga tgccgagctg cttcgcgaac atcgcggtgc gccagccgtt
     6481 cacgatcagg atcagcgcca ccgccagcgc gccgagcgcg gcccaggcgt cggcccagcg
     6541 ccgcgcgcgc ggcgaaagcc gcgcggtgag cagatccacg ccgatgtgct cgccgcgccg
     6601 cagcacctgc gccgcggcga gcatgacgat gccgaccagc atgaagccga cgatgtcgtc
     6661 gacccacacc ggcgcgcggt tgaacacgta gcgcatcacc accgcccagg cgatgagcgc
     6721 gagcgagacg agcacgcccg cggccgagag cgccgccgcg ccgtcggcga gggcgccgat
     6781 gacgcgcgcg gcgcgcgcgg cggcagcggc cgcgcgggtc atgccgcggc gctcagaggc
     6841 cgcggatcag ctcgagaagc cgcggtgcgt ccgggctcga ggcgctgaag gccttgatca
     6901 ccgcgggctg catcgcgtcg gccatcacct tcttctgccc cgcgctgagc tcggtgacgt
     6961 tcatgccctt gtccctgagc tgctgcaggt gctcgggcgg gatcgcgtcg tccttgggca
     7021 tgacgcgcgc ctcggccttc tccagcgcgt cgaggatgcc cttcttcacg tcggcgggga
     7081 gcttctccca ccactccggg ttcacgatca ggttgtcgta ggcgaggatg atgttcgagg
     7141 cgacgccgaa cttctgcacc tcgtagaagc gccggctgtg cgccgcctgc acgccggtga
     7201 agccggcgtc gatgacgccg gtctgcagtg cctggtacac ctccgagccc ggcatcgcag
     7261 agggcgctgc ccccatcgcg acgaggcctt cgtcccagag cttggacagg ccgcggatct
     7321 tcacgccctt gaaatcctcg ggcttgatga gcggcgcctt ggcggaagtg aagatgccat
     7381 cgttcgcgtc caccagccag gcgatgttct tcacgccctt ttccagcatc ttcgcgtcca
     7441 gcatcctcgc cgcctccgag ccggggaact tcttcatctt gtccaccgag ctcatgaagt
     7501 acgggatgat ggtgacgttc atctcgggga tcgtgccgcc ccactggaac gagagcacga
     7561 tcgccgactc gatcttgccg ccggcgaccg cggcgtggtg ctggttcggc ttgtagagct
     7621 gcgcggcgcc gaagatctgc acctcgacct tgccgttggt gttcgccttc acgtcggccg
     7681 cgaactgctc catgttcttc gccgagtggt gcgccggcgg gaactggtgc gacaggcgca
     7741 tggtgatgtc ggcggcctgc gcgcccgccg cgacgagcgt ggcgaacgcc gccaggccga
     7801 actttgcctt caggttcatt tcgtgccctc cttgagtttc tgccagaccg gccggaaatc
     7861 gtacgttgcg tagaactcgg tgttgaacgc gccccaggcg aacttctcca gcgtcaggtt
     7921 cacgtcgcgc agtcggctcg ccggcacgcg cagcgtcacc acctgcccga tgcccatcat
     7981 cacgtaccag gactcgacct cgatgccctg cggcgggaac tgcttccaga acccggccgc
     8041 gtcgagcttg gcgttgatct cgcccaggtt cttgtcctgc tggtgcttca ggaacaccgt
     8101 gagcaggatc tcgttctcag ccgccatgcg ccacctccca ccaaaaatta taaatgggga
     8161 cagacctcat tttccccgtt gtcgcctgtc gggcgcgcgg cgccccgatg aaaaatgagg
     8221 tctgtcccca tttaacttag gcgcagcggc agcttgcgga agccgcggaa gcgcacgcgc
     8281 cgatcccgct ccggcgctcc cgcgagctcg atgcggccga accgcgcgag cagccgcgcg
     8341 agcgcgatgc ggccctccat gcgcgcgacg ttcatgccga cgcacacgtg gtcgccctgg
     8401 ccgaaggcga ggtggcggtt gggcttgcgg ccgacgtcga agcgctcggg ctcgggaaac
     8461 tgcgccggat cgcggttcgc cgcgccgatg ccgatggtga tcagcgtgcc ctcgggcagc
     8521 gtgccggcgg cgagcgcgag cggcgcggtg aggcggcggt tattgagctg gatcgggccg
     8581 tcgtagcgca gcatctcctc gatcgcgctc ggcacgagcg acggatcggc gcgcagccgg
     8641 gcgagctccc cggggttgtt gagcagcgcc catacgccgt tgccgatcag gttggtggtg
     8701 gtctcgtgcc cggcgttgag caggaagatg cactggtggt agagctcctt ctcggtgagc
     8761 tgctcgccgt ccttctcgcc ctggatgagg cgcgtcagca catcggactc gtagtcgccg
     8821 gggtgcttgc gccgcgcggc gaccagccgc ttcagcaagt cgatgaactc gcgcaccgcg
     8881 cggttgcccg cctcgagcat ctccggcgtg agcttcggct ccaggcccga gaggatggcg
     8941 accgaccagc cgcgcagcgg cccgcgctcc tcgcgcggga tgtcgagcag gttgccgatg
     9001 acctcgatcg ggatctgcgc ggcgaagtcg tcgatgaaat tcacttcgcg cttcgcggcc
     9061 atctcctcca gcagcgtgtc gacgaggcgc gccacgttgg gctccatgcg cgcgatcgcc
     9121 ttctggttca ccgcgcccat gaggatgcgc ctgaccttcg tgtgcagcgg cgggtcgttg
     9181 aagacgaggc tcgtggtgtg gtgctcgtag agcggcgaat cgccgagctt cggcccgaac
     9241 tcggccttct tgtccgagct cgcctgcggc gcgcggtata cggcgagcgc gtcctcgtag
     9301 cgcgcgagga acacgccgcc gccttccagc gcgtgcaccg ggtcgcgctc gcgcagcgcc
     9361 gcgtaccacg ggtacgggtc gtcgagaaag tccgccggca cgcgcgtgag ctgaaagtcg
     9421 cggatcgcgt cggccgaggg cattcggcta ttatgccgcc atgcaacgcg tgcccaacac
     9481 cgagttcccg ccggagctca agaagcgcct cgaggagctc tggggctcgc caccgaacct
     9541 gtaccgcgcg ctcgccaacc acgcgccgct cgccgccgcg tggaccgagt tcgcgcgcgc
     9601 catccgctat gaaagccgca cgccgcgcac tctgcgcgag ctggtgatcc tgcgcggggc
     9661 gcagctcatg cgctccgagt acgagtgggc gcagcacctg aagatggcga ggaaggccgg
     9721 cgtgcgcgaa gcgcagatcg cggcgctcgc gctctggcgc gactcgggcg agttcgacgc
     9781 gacggagaag gcggcgctcg cactggccga ggcggtgacg cgcgccgacg tcacggacga
     9841 ggtgtacgcg gcggtgtcga agcacttcga ccatgccacc tacgtcgagc tcgcgctcac
     9901 cgcggcgttc tacgccatgg tcgggcgcat gctcgacgcg atgcgcgtgc agctcgagcc
     9961 cgagttccgc gacttccagc cgaagctgcc ctagagcgcg tgagcgccgg agcgccgcgg
    10021 cagtaccgct attacgagtt cgtgatggcg gcgttcgtca cggtgctgat ctgcgccaac
    10081 ctgatcgggc cggcgaagat ctcgcaggtg gagctgccgc tggtcggcac gctcgtcttc
    10141 ggcgccgggg tgctgttctt cccgatctcg tatgtcttcg gcgacatcct caccgaggtc
    10201 tacggctacg cgcgcgcgcg gcgcgtgatc tgggcgggct tcggcgggct cgcgttcgct
    10261 tccttcatgg cttacgtggt ggtggcgctg ccgccggcgc cgttctggaa gaaccagcaa
    10321 gcctacgaga tcgctttcgg ccaggcctgg cggatcgccg gcgcctcgat gatcgcgtac
    10381 ttctgcggcg agttcgtcaa ttcgttcgtg ctcgccaaga tgaagatcat gacggcgggc
    10441 cgctggctgt ggacgcgcac catcggctcg accatcgcgg gcgaggccgt ggactcggcg
    10501 ctgttctacc cgctcgcgtt ctacggcacc ggcatcatcc cggacgacaa gctcctcgcg
    10561 gtgatggcgt tccagttcgt cgccaaggtg ctggtcgagg tggcattcac gccgctcacc
    10621 tacaagatcg tggggctcct caagcgcgcc gagcgcgagg acttctacga ccgcaacacc
    10681 gacttcacgc cgttcagcct caggacatag cgacaaatca atcccggcag ccgcgggcgc
    10741 cgctcttgcg gttcctgacg catcagcggg tccgcgcgtc ggttcggcca ttgcattgca
    10801 gcgtgcggta gcggattctt ggcataccaa tgtcctatct caattgaaga tggtagccga
    10861 gcgactgccc gtgccgcgcc tcggacagtc cgcgccgttg cgcgtgctgc tgatcgaaga
    10921 caacaaggcc gacgcgatgg tggcgcaggc tgcgatcgaa aaagcggcga tcggtcgcgc
    10981 cgaagtcctg cgcgccgaga gcatcgcgcg ggggctcggc atcgcgggcg ccgcggaagt
    11041 gcacctcgtg ctgctcgacc tcaacttgcc cgacagccgc ggcctcgcca cgctggagcg
    11101 catgcgggcg gcggtgcgct gcccggtgat cgtcgtcacc gccgaggagc gcccggggct
    11161 cgacgaggag gcgctggaaa tgggcgcctt cgagatcctg cacaagggca agctcggcgc
    11221 aaacgccatc gcccgcgtgc tgcggctcgc cgagggccag cgcaaggtgc aggcctcgct
    11281 cgagagcgcc gagcagcgct accgcaagct cgtggagatc gcgccggacg cgatcttcgt
    11341 gcatgcggac tggcgcatcg cgctcgtcaa cccggcgatg ctgcgcctgt ttggcgccac
    11401 gcgcccggac gaactgctcg ggcgcgaggt gctcgagctg atcgcgccgg cagcgcgcgg
    11461 cgtggcgcgc gagcgcattc gtggcctcta cgagtcggcg cagtcggtgc agctgcggga
    11521 ggtcgagtac gtgcggcgcg acggcacgcc gttcagcagc gaggtcacgg cggtgtcgtt
    11581 caccttcgac ggccgccccg cggtgcaggt ggtggcgcgc gacatcaccg agcgcaagag
    11641 cgcggagctc gcgctgcgca ggagcgaaga gcgctttcgc ggcctgaccg agctatcgtc
    11701 ggactggtac tgggagcagg acaaggcgct gcggttcacc tacgtgtcgc cgggattcga
    11761 ggagcgctcc aagcacgacc cggcgctggt gctcggcaag cggcgctggg atttcgacaa
    11821 cgtcatgccg gaatcgggca cctgggacga ccaccggaag gtgctggagg cgcggcggcc
    11881 gttccgcgac ttcgagcaga tcaccgtgcg cccggacggc gcgcgcgtgc acatctcgag
    11941 cagcggcgag ccggtctacg acgagagcgg ggctttcacc ggctaccgcg gcgtgagcag
    12001 caacatctcg gtgcgcaagg cggccgaggc gcgcctggag cagctcgcgc agttcgactc
    12061 ggtgaccggg ctcgccaatc gcaacctgct gcatgagcgc ctggagcacg ccgtcgtgca
    12121 gtcgcggcgc cgcggccgcg gcgcgggcgt gctctatgtc aacctggatc gcttcaagct
    12181 ggtgaacgac acgcacgggc accacgtggg cgacgatctg ctgcgtcacg tcggccgccg
    12241 gctgcaggac tgcgtgcgcc gtgacgacac cgtgtcgcgc ctcggcagcg acgagtacgc
    12301 ggtggtgatc gcggatctcg cgcgcgccga cgacgccgcg ctcgtggcgc agaagctcct
    12361 cgacgcgctc gccgcgccgt tcgacctggg cggacgcgaa accttcatca ccgcgagcat
    12421 cggcgtcgcg acgttcccga ccgacggcga gagcgccgaa gcgctgctga agtgcgccga
    12481 catcgccatg ggccgcgcca aggaatcggc gcgcaacacg ttctgcttct acaccgcgga
    12541 gctcaatgcg cgcgcggcct ccaagctgca gctcaattcc gacctgcggc gcgcgctcga
    12601 gcgcggcgag ttcctgctgc actaccagcc caaggtgcgc ctggcgagcg ggcagatgat
    12661 cggcatggag gcgctgctgc gctggcagca cgcgcagcgc ggcatggtgg cgccgctgga
    12721 attcatcccg gcgctcgaag aaagcggcca gatcgtgccg gtgggcgact gggtgatcgc
    12781 gcaagcctgc cggcagctcg ccgaatggtt gcgcgcgggc ctgcagtcgg cgccggtggc
    12841 ggtcaacctc tcggcgcgcc agtttcgccg ccgcgacctc gaccgcgtga tcaaggacat
    12901 gcttgccgag cacggcgtgg cgcccgggat gctcgagctg gagatcaccg agagcagcct
    12961 gatggacgat cccaaggacg cgatccgcca gctgcacgcg ctgcgcgagg cgggcctgcg
    13021 catttcggtg gacgacttcg gcaccggcta ctcgagcctg tcgtacctga cgcgcctgcc
    13081 ggtctccaca ctcaagatcg accgcgcctt cgtcaatgcg gcgatccgcg agcccagctc
    13141 ggcggcgatc gtgcgcatgg tgatcgacat ggcgctgcgc ctgcgcttcg acgtcgtggc
    13201 cgagggcatc gagagcgagg agcacgtggc gttcctgcgc gagcacggct gcgagcaggg
    13261 ccagggctat cacttcggcc ggccgatccc cgccgccgag atggcccgcc gccttaaata
    13321 gggacagacc tcatttttcg ttgtccgcgc atcaagcgcg ggcggaaaaa tgaggtctgt
    13381 ccctatttaa gccccgcgca gcaggcgcgg gcggaaaaat gaggtctgtc cctattttgc
    13441 ctcgtagacc cagcgcagga gggcgtcgtt ggcggcttcg gcctctggcg cgttggggtg
    13501 gttcatgagc atgacgacga tctggcgccg gcccttcgcg tcgaggaggt agccggccag
    13561 cgcgcgcgcg tccgagagca gccccgtctt catgtgcgct tgtcccgcga ccggctcgcc
    13621 gtgcaggcgc tttctcatcg tgccgtccag cgctgcgagc ggcagcgagg cgatgtactc
    13681 gggcatcacc gcgctcttcc acgccgcctg cagcagcgcg gcgagggtcg ccgcgctcgc
    13741 gcggtcggtg cgcgagagcc ccgagccgtt ctcgatcgag agctcgggcg cggcgatgcc
    13801 cttcaggttc agccagtggc gcagcacctg ctgcgcctgg tgcagctgcg ccggcggctc
    13861 gtagcccgcg gtggccagcg tgagaaagag ctgccgtgcc atcacgttgt tggagaactt
    13921 gttgacgtcg cgcacgatct cggcgagcgg cgccgattcg tgcgtgtgca gcagctgcgc
    13981 cgcctccggg accgcgcctt cgcgcgcgcg cccgctccag gcgccgccca tttccgacca
    14041 cagctcgcgc agcacgccgc cgacgtgctc gtcgggcggc aggaaggcga cgttgaggtc
    14101 cttttcgccg caggcgatcg ggtagcgtcc ctcgaaggcg gcgcgcggcg gcgggccggg
    14161 ctcgaagctg gcctgcagca gggtgcggaa cgcgcgcccc tcgaagcagg ggccggaaga
    14221 caggcgcagg ctgttcttca cctcgagcgc cggcgggcgc ggctcggcgt agatgcgcac
    14281 gcccgcgccg tcggcgagcg gcacgaaggc aaagcgcagc gccttgtagt gcacgagaag
    14341 cgcatccggc agcacgttgt agggccgcag cgcctcgccg tcgaagtcgc ccggatcgcc
    14401 cgccagcggc gcaaagaagc ggcgatcgat cagcacgtcg ccgcggatct cgcgcaggcc
    14461 cttgccgcgc agcgtgcgca acagcatcca gaagctctcg tagtcgagct tcgggtcgcc
    14521 gtagcctttc aggacgaggt cgccttcgag cacgccatcg cgcagcggcc cggcggcgta
    14581 gacctcggtc ttccagtggt aggccggccc gagaagctcg agcgccgcga gtgtggtgaa
    14641 cagcttcatc gccgaggcag gattcatcgc cgcctcggcg ttcatgctca ccgcagggcg
    14701 ggcggcgcct acctcctgca cgacgaccgc gaccgcccgg agcggcacgg aagccgcgcg
    14761 cagcgcctcg gccaccggct tggggagtcc gctcgccgct gcggtggacg gcagcggcgc
    14821 cagcagcagg acggcggcga gcgccagacg gcagaagcgg ggcacgcgcg aattctagtg
    14881 ggcgcgacgc gaccggccct tgtattcccc cgtcctcgcc cctatcatta gcactcgctt
    14941 gaggcgagtg ctagcaagcc tctcgtattt ggcgggttgc acaactgcaa cccattgact
    15001 tacaacataa atgtctggag acctgtatga agatccgtcc tcttcatgat cgcgtcatcg
    15061 tcaagcgcat cgacgaggag cgcaagaccg cgtctggaat cgtgattccc gacacggccg
    15121 ccgagaaacc cgaccagggc gaagtcctgg ccgtcggcaa aggcaagaaa accgatgacg
    15181 gcaagctgat ccagctcgac gtcaaggtgg gagacaaagt gctgttcggc aagtactccg
    15241 gacagaccgt gaaggtgaag ggcgacgagc tgctcgtgat gcgcgaagaa gacatcatgg
    15301 gcgtgctcga aggcgcctga gcgtcaacac gaaccttttt ctgatctagg agaattcgca
    15361 atgcctgcca aagaagtacg ctttcacgac agcgcgcgca cccgcatggt ccatggaatg
    15421 aacatcctgg ccgacgctgt caaagtcacc ctcggcccca agggccgcaa cgtcgtgctc
    15481 gagcgctcgt tcggcgcccc caccgtcacc aaggacggcg tatcggtggc caaggaaatc
    15541 gagctcaagg acaagttcga gaacatgggc gcgcagatgg tgaaggaagt cgcctccaag
    15601 acctcggacg ttgccggcga cggcaccacc accgccaccg tgctggcgca agccatcgtg
    15661 cgcgagggca tgaagttcgt cgcctcgggc atgaatccga tggacctgaa gcgcggcatc
    15721 gacaaggccg tgatcgcggt caccgatgag ctgaagaaga tctcgcagcc ctgcaagacg
    15781 tccaaggaaa tcgcccaggt cggctcgatc tcggcgaact ccgattccga catcggcaag
    15841 atcatcgccg acgccatgga caaggtcggc aaggaaggcg tgatcacggt cgaggacggc
    15901 aagagcctgc agaacgagct cgaagtcgtc gagggcatgc agttcgaccg cggctacctg
    15961 tcgccctact tcatcaacaa ccccgagaag cagatcgcgg tgctcgatga cccgtacctg
    16021 ctcctgcacg acaagaagat cagctcgatc cgcgagctgc tgccgatcct cgagcaggtg
    16081 gcgaaatcgg gcaagccgct ggtcatcatc gccgaggaag tcgaaggcga ggcgctcgcc
    16141 accctggtgg tgaacaacat ccgcggcatc ctcaagaccg tggccgtgaa ggcgccgggc
    16201 ttcggcgatc gcaggaaggc gatgctggaa gacatggcga tcctcaccgg cggcacggtg
    16261 atcagcgaag agctcggcct gaagctggag aacgcgacgc tgaaggacct cggcaaggcg
    16321 aagaagatcg aggccggcaa ggaaaacacc accatcatcg atggcgccgg cgtgaagaag
    16381 gacatcgagg gacgcgtcaa gcagatccgc gtgcaggttg aagaggcgac cagcgactac
    16441 gacaaggaaa agctccagga gcgcgtggcg aagctcgccg gcggcgtggc gctgatcaag
    16501 gtcggcgcgg ccaccgaagt cgagatgaag gagaagaagg cgcgcgtcga agacgcgctg
    16561 cacgcaaccc gcgcggccgt ggaagagggc atcgtccccg gcggcggcgt ggcgttcctg
    16621 cgcgcgagga aggcgctcga aggcaagctc aagggcgaaa accacgacca ggacgccggc
    16681 atcaagatcg tgatgcgcgc actcgaggaa ccgacgcgcc agatcgtcga aaatagcggc
    16741 gccgagccgt ccgtggtcct caacaagatc atggaaggca aaggcgtgaa ctacggcttc
    16801 aacgcgcaga ccgacgagta cggcgacctg gtggatatgg gcgtgatcga tcccaccaag
    16861 gtggcgcgca cggcgctgca gaacgcctcc tccgtggccg gcctgatgct caccaccgac
    16921 gcgatggtgg cggagctggt ggaggagaag aagggcggcg gccatgcgca tccggacatg
    16981 ggcggcatgg gcggcatggg tggcatggac atgtaacccc tagggacagg ggaaaccgag
    17041 gtttcccccg taccgccatc ctgaaaaccg aagaggggcc cgagcgggcc cctcttcttt
    17101 tataatccgc gctcttcccg gcaggccaag tagctcagtt ggtagagcag cggactgaaa
    17161 atccgtgtgt cgctggttcg attccggcct tggccaccaa cctttccgcg cgcgagaccg
    17221 ccccgatgaa ggcgcgccgg aggacatcgc gctacggcga cgaggtgccc cggccttcgt
    17281 aagagcttga tctaacgcaa ttggcgaggg ggcttgcttc tataatgcgc aattggcgca
    17341 ttcgatgacc ctcttcgccc tcggcctcag ccacaccacc gcgccgctgc cggtgcgcga
    17401 gcgcgtggtg ttccacgtcg aaaggctcta cgacgcactc ggcgagctga cgcgcggcca
    17461 gcgggtggcc gaagccgcca ttctctccac ctgcaaccgc accgagctgt acctgtcggc
    17521 gggcgagccg cagaccgcgg ccgaatggtt cgcgcgctac cacagcctgc agcccgagga
    17581 gctgcggccc tacctgtaca ccctgccgca ggaccaggcc gtgcgccaca ccttccgcgt
    17641 cgccgcgggg ctggattcga tggtgttcgg cgagccgcag atcctggggc agctcaagga
    17701 cgcggtgcgc accgcggaag ccgcgggcac gctcggcacg gtgctgcaca agctgtttca
    17761 gcgcaccttc gcggtggcca aggaggtgcg cagcaccacg ccgatcggcg ccagcagcgt
    17821 ttccatggcg gcggcggcgg tcaagctcgc ggcgcgcatc ttcccgagcc tgcgggagca
    17881 aaaggtgctg ttcatcggcg cgggcgagat gatcgagctc tgcgccacgc actttgcggc
    17941 gcaggcgccg gcgcgcatcg cggtggccaa ccgcacgctg gagcgcgcgc aggcgctcgc
    18001 gcaccgcttc gccgggcacg cggtcgagct gcgcgcgctc gccgagcacc tgcaggacta
    18061 cgacatcgtc atctcctgca ccgcgtcctc gctccccatc ctcggcaagg gcctggtcga
    18121 gcgcgcgctg cgcgcgcgca agcgccgccc catgttcatg gtggacct
//
LOCUS       DBNHKE010000032         8185 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1432763, whole genome shotgun sequence.
ACCESSION   DBNHKE010000032 DBNHKE010000000
VERSION     DBNHKE010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1432763"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="lpxB"
                     /locus_tag="AC16O9_01905"
     CDS             complement(<1..802)
                     /gene="lpxB"
                     /locus_tag="AC16O9_01905"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR6515405.1"
                     /translation="MAEGANHIAMVAGEASGDQLGAHLIAALKARRPQLRFSGIGGPR
                     MAAQGFEVSVPMDRLAVRGYVEALRHYREIMRIRARLGEALLQAPPGLFIGVDASDFN
                     LDLERRLKQAGIPTVHYVSPSVWAWRRWRVRRVARSATHLLAMFPFEPALYEKSGLPV
                     TYVGHPLADVIALKIDKAEAKSQLRLPARKLVVALLPGSRRSELEHMAQTFILAAHRF
                     RQEVPEVHFVCPLATRETRDLFEATLHAHQRTDLPLTLLFGHSHEALAA"
     gene            complement(807..1589)
                     /gene="lpxA"
                     /locus_tag="AC16O9_01910"
     CDS             complement(807..1589)
                     /gene="lpxA"
                     /locus_tag="AC16O9_01910"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR6515406.1"
                     /translation="MSAAQVHATAIVHPGAKLAPGVTVGAYSLIGENVEIGEASWIGP
                     HVVLDGRVRVGRGNRIYQFASIGAPPQDKKYAGEDTAVEIGDRNTIREYVTINRGTAR
                     DAGVTRVGDDNWIMAYVHFAHDCQIGSQTIFANACELAGHVHVGDWAILGATTLVHQF
                     VHIGAHSFTGMGTYLPQDLPPYVKAAGNMAKPYGINSEGLRRRGFSADTIARIKRAYR
                     TLYRAGLSLEEAKRELAAQAADCPEVGALLEFLARSKRGFIR"
     gene            complement(1586..2038)
                     /gene="fabZ"
                     /locus_tag="AC16O9_01915"
     CDS             complement(1586..2038)
                     /gene="fabZ"
                     /locus_tag="AC16O9_01915"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR6515407.1"
                     /translation="MPEMDAQQVLDYLPQRYPLLMIDRVLECEPGKRILALKNVTNNE
                     PQFTGHFPGRPIMPGVLMLEAMAQAAGILVFRTRGDKADGNTVYYLAGIDEARFKKPV
                     VPGDRIEIEATILAHKKTIWKFACVARVDGAVASEAVILCTERVQRQG"
     gene            complement(2031..3074)
                     /gene="lpxD"
                     /locus_tag="AC16O9_01920"
     CDS             complement(2031..3074)
                     /gene="lpxD"
                     /locus_tag="AC16O9_01920"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR6515408.1"
                     /translation="MAGPISLGEIASRLGGRVVGDAGVLVEQVASLEHARGHEIAFFS
                     SARYKAQLAATRAAAVILGPQAEPLTRLPRIVSDNPYAYFARVSQLLNPAGAPQPGVH
                     AAAHVDASARVHASARIDAGAVVGARAEIGERVWIGAGCCIGEGAVIGADSRLHPSVV
                     IYEGCRIGKRALVHSGAVIGADGFGMALDAGRWLKVPQIGRVLVGDDVEIGANTTIDR
                     GTVDDTVIEDGVKLDNQIQIGHNCRIGAHTAMAGCAGVAGSAVIGQRCTIGGAAAILG
                     HLSICDDVHVSAVTVISRSIRKPGTYTGIYPFDDNASWARNAALVRHLAELADRVGAL
                     EKKSGSRRKKRNA"
     gene            complement(3076..3600)
                     /locus_tag="AC16O9_01925"
     CDS             complement(3076..3600)
                     /locus_tag="AC16O9_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR6515409.1"
                     /translation="MHKLFAVLAPSLALALALWAPPAAAQSEGRIGFVNTARILRDAA
                     PAVRAQKKIEAEFSKRDQELAKTAEQLKRMQDELERQGVTMSESQRRTKEREFNDLNR
                     DFQRRQREFREDLNQRRNEELAQVVEQANRVIRQIAEQEKYDIIFQDAVYANPRIDIT
                     DRVIKALEGKPPAR"
     gene            complement(3616..5889)
                     /gene="bamA"
                     /locus_tag="AC16O9_01930"
     CDS             complement(3616..5889)
                     /gene="bamA"
                     /locus_tag="AC16O9_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR6515410.1"
                     /translation="MTAALAWALLAVASAVAAQEFQPFKVLDIRVEGVQRTDPGTVFS
                     YLPVKVGETLTADKAQQALRALFATGFFKDVRLEAEGGVLVVLVEERPAIAQVDFSGV
                     SEFPLDNLRRALRDLGLAEGRIFDRAVLENAEQELKRQYLSRGKYAAEVQTTVTPLER
                     NRVGISFAVSEGDVAKIRAINIVGAQAFKEGTLLDLFVLRTPGWLTWYTKHDQYSRQR
                     LSADLEALRSFYLDQGYLDFNVESTQVSITPDRKDIYITVNIVEGAKYTVSDIRIGGQ
                     TLVPSDALEKLLRIGPGDVFSRQKLIESQKAITDRLGNDGYAFANANAVPEIDRDKRT
                     VAFTLLIDPGRRVYVRRINVVGNSKTRDEVVRREMRQLEGAYYDASKIQLSRRRIDRT
                     RYFSDVQVATAPVEGRPDQVDVTYTVEERSTGSLALGVGFSTLDKVILSASITQPNVF
                     GTGKYLSLELNSGTVNQVYALSYVDPYYTVDGVSRGFDFYKRSVDATTLATGAYGTNT
                     LGGGIKFAYPLSERVTVSFGALAESVEVVTFENSAQVYKDFVAAFGNDYTYGAFTAAW
                     ARDTRDSLIVTTDGLLTRFSTELAGGDLQYYRLGINQQLYWPLSRTFTLFLNADLGYA
                     SGSGGKTLPFFKNYYAGGPGTVRGYDDFSLGPRDAAGNTLGGTRRVVGNVELLFPMPG
                     AAQDKSLRVGWFVDAGQVYADGAPVDLSELRYSTGLALAWASPLGPLRLSYATPLNER
                     EGIDRVQRLQLKFGTIF"
     gene            complement(5916..7280)
                     /gene="rseP"
                     /locus_tag="AC16O9_01935"
     CDS             complement(5916..7280)
                     /gene="rseP"
                     /locus_tag="AC16O9_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR6515411.1"
                     /translation="MSFLHTVVSFIVALGVLIVVHEYGHYLVARLCGVKVLRFSVGFG
                     RALATWRKGRDQTEWVIAAIPFGGYVKMLDEREAPVAPHEAGRAFNRQSVLKRLLIVV
                     AGPAFNFVFAIAVYAGLYMVGLPEARPVLAEPPAATLAHAAGVRGGDRVRAVDGEAIA
                     TWQELRWRVLQAALQREPMALEIINARDQISSLTLDLGDYPAEEVESDVLERIGLRLY
                     RPSLAPVLGQVVTGSAAARAGLEPGDRVTHIDDRPVATWEDLVREVRRRPDQEARLRI
                     ERGELSMTLSVTPEAVAVNGERLGRIGAAPAIPADHAERIFVQVRHSPLDAVAQAAVK
                     TWDISVFSLRMLGRMLLGQVSWKNLSGPVTIADFAGQSAQLGWIPYLTFLALISISLG
                     VLNLLPIPPLDGGHLMFYVIEILKGSPASERAIEFGQRVGIALLLVLMAFAFYNDLTR
                     LFAG"
     gene            complement(7277..>8185)
                     /gene="dxr"
                     /locus_tag="AC16O9_01940"
     CDS             complement(7277..>8185)
                     /gene="dxr"
                     /locus_tag="AC16O9_01940"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR6515412.1"
                     /translation="PEGDTVMAAIVGAAGLPATLAAARAGKRVLLANKEALVMAGPLF
                     MRAARDAGAVLLPIDSEHNAVFQCLPPEGARMQGVRRIQLTASGGPFRATPAERLAGV
                     TPEEACAHPNWVMGRKISVDSATMMNKGLEVIEAHWLFGLPAERIDVLIHPQSVVHSL
                     VEYLDGSVIAQLSHPDMRVPIAHALGFPERIESGARSLDLGAVGHLSFEAPDAARFPC
                     LGLAYGALRAGGTGPALLNAANEIAVEAFLAGRLRFTGIAPVIEETLDRVAAGAAASL
                     EQVMEADARARRAAEDCVVRLMGQAA"
BASE COUNT         1306 a         2809 c         2779 g         1291 t
ORIGIN      
        1 cggcggcgag cgcttcatgc gaatggccaa acagcagcgt cagcggcagg tcggtgcgct
       61 ggtgcgcgtg cagcgtcgcc tcgaacaagt cgcgcgtctc gcgcgtggcc agaggacaca
      121 cgaagtgcac ttccggcacc tcctggcgaa agcgatgcgc ggcaaggatg aacgtctggg
      181 ccatgtgctc gagctccgag cgtcggctgc cgggcagcag cgcgaccacc agcttgcgcg
      241 caggcaggcg cagctgcgac ttggcctccg ccttgtcgat cttgagcgca atgacgtcgg
      301 cgagcggatg gccgacgtaa gtcaccggca ggccggactt ctcgtacaaa gcgggctcga
      361 acggaaacat cgccaggagg tgcgtcgccg aacgggcgac gcgccgcacg cgccagcgcc
      421 gccaggccca caccgagggg ctgacgtagt gcacggtcgg aatccccgcc tgcttgaggc
      481 ggcgctccag gtcgagattg aagtccgaag catcgacgcc gatgaacaat cccgggggcg
      541 cctgcagcaa ggcttcgccg agccgggcgc gaatgcgcat gatttcgcgg tagtggcgca
      601 gcgcctcgac gtagccgcgc acggccagcc ggtccatggg cacgctgacc tcgaaaccct
      661 gcgccgccat gcgtgggccg ccgatgccgg agaagcgcaa ctgcggccgg cgcgccttga
      721 gcgccgcgat gagatgagcg ccaagctgat cgccggacgc ctcgccggca accatggcga
      781 tgtggttggc gccctccgcc acggcgctag cggatgaagc cgcgcttcga gcgcgccagg
      841 aactcgagca gcgcgccgac ctccggacaa tccgcggcct gcgccgccag ctcccgcttc
      901 gcctcctcca ggctcaggcc ggcgcgatac agggtgcgat aagcgcgctt gatgcgcgcg
      961 atggtgtcgg cgctgaagcc gcgacggcgc aggccttcgc tgttgatgcc gtacggcttg
     1021 gccatgttgc cggcggcctt cacgtacggc ggcaggtcct gcggcaggta ggttcccatg
     1081 ccggtgaagc tgtgcgcgcc gatatgcacg aactggtgca ccagcgtggt cgcgccgagg
     1141 atcgcccagt cgcccacgtg cacgtggccg gcgagctcgc aggcgttggc gaagatcgtc
     1201 tggctgccga tctggcagtc gtgggcaaag tgcacgtacg ccatgatcca gttgtcgtcg
     1261 ccgacgcggg tcacgccggc gtcgcgcgcg gtgccgcggt ttatggtcac gtactcgcgg
     1321 atcgtattgc ggtcgccgat ctcgaccgcg gtgtcttcgc cggcgtactt cttgtcctgc
     1381 ggcggcgcgc cgatggaggc gaactggtag atgcggttgc cgcggccgac acgcacgcgt
     1441 ccgtcgagaa ccacgtgcgg gccgatccag ctggcctcgc cgatctcgac gttttcgcca
     1501 atcagggagt aggcgccgac cgtgaccccc ggcgcgagct tcgcgccggg gtggacgatc
     1561 gccgtggcgt gaacctgcgc ggcgctcacc cctgcctctg gacgcgctcg gtgcacagga
     1621 tcacggcctc gctagcgacg gcgccgtcga cgcgcgccac gcaggcgaac ttccagatcg
     1681 tcttcttgtg cgcaaggatg gtggcctcga tctcgatccg gtctccgggc acgaccggct
     1741 tcttgaagcg cgcctcgtcg atgccagcca ggtaatacac ggtgttcccg tccgccttgt
     1801 cgccgcgcgt gcgaaacacc aggatgcctg cggcctgcgc catcgcctcg agcatcaaca
     1861 cgcccggcat gattggccga ccggggaagt gcccggtaaa ctgcggctcg ttgttggtga
     1921 cgttcttcag cgcgaggatg cgcttaccgg gctcgcactc gagcacgcgg tcgatcatca
     1981 gcagcggata gcgctgcggc aggtagtcga ggacctgctg ggcatccatc tcaggcattg
     2041 cgcttcttcc tccttgagcc gctcttcttt tccagcgcgc caacgcgatc ggcgagctcc
     2101 gcgagatgcc gcaccagggc ggcattgcgc gcccaggagg cattgtcgtc gaacgggtaa
     2161 atgcccgtgt aggtgccggg cttgcggatc gatcgcgaga tcaccgtaac agcggacacg
     2221 tgcacgtcgt cgcagatgct caggtggccg aggatcgccg ccgcgccccc gatggtgcag
     2281 cgctggccga tgaccgcgct gccggcgaca cccgcgcagc ccgccatcgc cgtatgcgcg
     2341 ccgatgcggc agttgtggcc gatctggatc tggttgtcga gcttgacgcc gtcctcgatc
     2401 acggtgtcgt ccacggtgcc gcggtcgatg gtggtgttgg cgccgatctc gacatcgtcg
     2461 cccaccagca ctcggccgat ctgcggcacc ttgagccaac gccccgcatc gagcgccatg
     2521 ccgaagccgt cggcgccgat caccgcgccg gaatgcacga gggcgcgctt gccgatgcgg
     2581 caaccctcgt agatcaccac ggaagggtgc aggcggctgt cggcgccgat caccgcccct
     2641 tcgccgatgc agcagcccgc gccgatccac acgcgctcgc cgatctccgc acgcgcgccc
     2701 accaccgcgc cggcgtcgat gcgcgcactg gcgtggacgc gcgcgctggc gtcgacatgc
     2761 gccgccgcat gcacgccggg ctgcggggca cccgccggat tgagcagttg cgacacgcgc
     2821 gcgaaatagg cataagggtt gtcgctgacg atgcgcggca gccgggtgag cggctcggcc
     2881 tgcggcccga gaatcacggc agcggcacgc gtggcagcga gctgcgcctt gtagcgcgcg
     2941 ctcgaaaaga aggcgatctc gtgcccccgc gcgtgctcca gcgaggccac ctgctccacc
     3001 aggaccccgg cgtcgccgac cacgcgcccg cctaacctgg atgcgatctc gccgagggaa
     3061 atcgggcccg ccatgtcagc gcgcgggcgg cttgccctcg agcgccttga tcacgcggtc
     3121 ggtgatgtcg atccgcgggt tcgcgtacac ggcgtcctgg aagatgatgt cgtacttctc
     3181 ctgttccgcg atctggcgga tgacccggtt tgcctgctcc accacctgcg ccagctcttc
     3241 gttgcggcgc tggttcaggt cctcgcggaa ctcgcgctgg cggcgctgga agtcgcggtt
     3301 caggtcgttg aactcgcgct ccttcgtgcg ccgctggctt tccgacatgg tgacgccctg
     3361 gcgctcgagc tcgtcctgca tgcgctttag ctgttcggcg gtcttggcca gctcctggtc
     3421 gcgcttggag aattcggcct cgatcttctt ttgcgcgcgc accgccggcg cggcgtcacg
     3481 caggatgcgc gccgtattga cgaagccgat acgcccctcg gattgcgcgg cggcgggcgg
     3541 tgcccacagc gcaagcgcca gggccagaga aggagccaga accgcgaaga gcttgtgcat
     3601 gaatcgcccg atggcctaaa agatggtgcc gaacttcaat tgtaacctct gcacgcgatc
     3661 gatcccttcg cgctcgttca gcggcgtggc gtacgatagc cgcagcggcc cgagcggcga
     3721 cgcccaggcc aacgccaggc ccgtcgaata gcgcagctcc gacagatcga ccggggcgcc
     3781 gtccgcatag acctgcccgg catcgacgaa ccagccgacc cgcagcgact tgtcctgcgc
     3841 cgcgcccggc atcggaaaca gtagctcgac gttgccgaca acgcgacgcg tgccgccgag
     3901 cgtgttgcca gcggcgtcgc gcggcccaag cgagaaatcg tcgtagccgc gcacggtgcc
     3961 ggggccgccc gcgtagtagt tcttgaagaa cggcagggtc ttgccgccgc tgccgctggc
     4021 atagccgagg tcggcattca ggaacagcgt gaacgtgcgg ctgagcggcc agtacagctg
     4081 ctggttgatg ccgaggcggt agtactgcag gtcaccgccc gccagctcgg tgctgaagcg
     4141 ggtcagcagg ccgtccgtgg tgacgatcag gctgtcgcgc gtatcgcgtg cccacgccgc
     4201 cgtgaacgcg ccgtacgtgt agtcgttgcc gaaggcggcg acgaaatcct tgtagacctg
     4261 tgcgctgttc tcgaacgtaa ccacctcgac gctctccgca agcgcgccga agctcacggt
     4321 cacgcgctct gacagcggat aagcgaactt gatgccgccg ccgagcgtat tggtgccgta
     4381 ggcgccggta gcgagggtcg tggcgtcgac cgagcgcttg tagaagtcga atccgcggct
     4441 gacgccatcg accgtgtagt acgggtcgac gtaggacagc gcgtagacct ggttgacggt
     4501 accgctgttt agctccagcg aaaggtactt gccggtgccg aagacgttgg gctgcgtaat
     4561 ggaggccgag aggatgacct tgtccagcgt ggaaaagccc acgccgagcg caagcgagcc
     4621 ggtcgagcgc tcctcgaccg tgtaggtcac gtcgacctgg tcgggccggc cttcgaccgg
     4681 cgcggtggcg acctgcacgt cgctgaaata tcgggtgcgg tcgatgcgcc gtcgcgacag
     4741 ctggatcttc gacgcgtcgt agtaggcgcc ttcgagctgg cgcatctcgc ggcgcacgac
     4801 ctcgtcgcgc gtcttgctgt tgccgacgac gttgatgcgg cgtacgtaaa cccgccgccc
     4861 agggtcgatc aacagggtga acgccaccgt gcgcttgtcc cggtcgatct ccggcacggc
     4921 gttggcattc gcgaatgcat aaccgtcgtt gccgagccgg tcggtgatcg ctttctgcga
     4981 ctcgatcagc ttctggcgcg agaagacgtc gccaggcccg atgcgcagaa gcttctccag
     5041 cgcgtcgctg gggaccagcg tctgcccgcc gatgcggatg tccgacacgg tgtacttcgc
     5101 cccctcgacg atattgaccg tgatgtagat gtccttgcgg tcaggcgtga tcgaaacctg
     5161 ggtggactcg acgttgaagt cgaggtagcc ctggtcgagg tagaaggaac gcagcgcctc
     5221 caggtcggcc gacaggcgct ggcgcgagta ctggtcgtgc ttggtgtacc aggtcagcca
     5281 gcccggggtg cgcaggacga acaggtcgag cagcgtgcct tctttgaagg cttgcgcgcc
     5341 gacgatgttg atggcgcgga tcttcgccac gtcgccttcg ctgacggcaa agctgatccc
     5401 cacgcggttg cgctcgagcg gcgtaaccgt ggtctgcacc tcggcggcgt acttgccgcg
     5461 cgacaggtac tggcgcttga gctcctgctc cgcgttctcg agcaccgcgc ggtcgaagat
     5521 gcgcccttcg gcgaggccca agtcgcgcag cgcgcgccgc aggttgtcga gcgggaattc
     5581 gctcacgccg gaaaaatcca cctgggcgat cgccgggcgc tcttccacca gcaccaccag
     5641 cacgccgccc tccgcctcga gccgcacgtc cttgaagaaa ccggtggcga acagcgcgcg
     5701 cagcgcctgc tgcgccttgt ccgcggtcag cgtctcgccc accttgacgg ggagatagct
     5761 aaacacggta ccgggatcgg tgcgctgaac gccctcgacg cggatgtcca gcaccttgaa
     5821 cggctggaac tcttgggcgg cgacggcact cgctaccgcc agcagcgccc aagcgagcgc
     5881 cgcagtcatg cgcttacaca tcggcgatca cgctttcagc cggcgaacaa gcgcgtcagg
     5941 tcgttgtaga aggcaaacgc catcagcacc agcagcaaag cgatgccgac gcgctggccg
     6001 aactcgatgg cacgctccga cgccgggctg cccttgagaa tctctatgac atagaacatg
     6061 agatggccgc cgtcgagcgg cgggatgggc agcaggttga gcacgccgag gctgatgctg
     6121 atcagggcaa ggaacgtcag gtaggggatc cagccaagct gcgccgattg cccggcgaaa
     6181 tcggcgatcg tcaccggccc ggacaggttc ttccaggaca cctggccgag cagcatcctg
     6241 ccgagcatgc gcaggctgaa caccgaaatg tcccaggtct tgaccgccgc ctgcgctacg
     6301 gcgtcgagcg ggctgtggcg cacctgcacg aagatgcgct cggcatggtc ggccggaatc
     6361 gccggggccg cgccgatgcg cccgaggcgc tcgccattca cggctacggc ttcgggcgtc
     6421 accgacagcg tcatgctgag ctcgccgcgc tcgatgcgca ggcgcgcctc ctgatcgggc
     6481 cggcggcgta cctcgcgcac caggtcttcc caggtggcca ccgggcgatc gtcgatgtgg
     6541 gtaacccggt cgcccggctc cagcccggcg cgtgccgccg cgctgccggt aaccacctgg
     6601 ccgagcaccg gcgccagcga cggccgatac aagcgcaggc cgatgcgttc gaggacgtcg
     6661 ctttccactt cctcggccgg gtaatcgccc aggtccaggg tcaggctgga gatctggtcg
     6721 cgcgcgttga tgatctcgag cgccatcggc tcgcgctgca acgccgcctg cagcacgcgc
     6781 cagcgcaact cctgccacgt ggcgatcgcc tcaccgtcga cggcgcgcac ccggtcgccg
     6841 ccccgcacgc ccgccgcatg ggcgagcgtc gccgccggcg gctcggcgag caccggccgc
     6901 gcttccggca gcccgaccat gtacaggccc gcgtagacgg cgatggcgaa cacgaaattg
     6961 aacgcggggc cggcgaccac gatgagcagg cgcttgagca cgctctgccg gttgaaagcg
     7021 cgccccgctt cgtgcggtgc caccggcgct tcgcgctcgt ccagcatctt gacgtagccg
     7081 ccgaatggga tcgccgcgat cacccactcg gtttggtcgc gccccttgcg ccaggtggcg
     7141 agcgctcgtc cgaagcccac cgagaagcgc agcaccttca cgccgcacag ccgcgcgacc
     7201 aggtagtgcc cgtattcgtg caccacgatc agcacgccga gcgcgacgat gaacgacacc
     7261 acggtgtgca ggaaactcat gccgcctgcc ccatcagccg cacgacgcaa tcctcggcgg
     7321 cacgccgcgc acgggcgtcc gcctccatca cctgctcgag cgacgcggcc gccccggcgg
     7381 cgacccgatc cagcgtctcc tcgatgacgg gcgcgatgcc ggtgaatcgc agccggccgg
     7441 cgagaaacgc ttccactgcg atctcgttgg cggcgttgag gagcgcgggg ccggtgccgc
     7501 cggcgcgcaa cgccccgtag gccagtccga ggcaggggaa gcgcgccgca tcgggcgcct
     7561 cgaagctgag atggcccacg gcgcccagat cgagcgatcg tgcgccagac tcgatgcgct
     7621 cgggaaagcc gagcgcatgg gcgataggca cgcgcatgtc gggatgcgac agctgcgcga
     7681 tcaccgagcc gtccaggtac tcgaccagag agtgcacgac gctttgcggg tggatcagca
     7741 cgtcgatgcg ctcggccggc aatccgaata gccagtgcgc ctcgatcact tccaggccct
     7801 tgttcatcat ggtggccgag tccaccgaga tcttgcgccc catgacccag ttcgggtggg
     7861 cgcaggcctc ttccggggtg acgccggcca ggcgctcggc cggcgtggcc cggaacggcc
     7921 cgcccgaggc ggtgagctga atgcggcgca cgccctgcat cctggcgccc tcggggggca
     7981 ggcactggaa caccgcgttg tgctcgctgt cgatcggcag cagcaccgcg cccgcgtcgc
     8041 gcgctgcgcg catgaacaac ggcccggcca tgaccagcgc ttccttgttg gcaaggagca
     8101 cgcgtttgcc ggcacgtgct gccgccagcg tcgcgggcaa gccggcggca ccgacaatcg
     8161 ccgccatgac cgtgtcgccc tcggg
//
LOCUS       DBNHKE010000033        16546 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_4943, whole genome shotgun sequence.
ACCESSION   DBNHKE010000033 DBNHKE010000000
VERSION     DBNHKE010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_4943"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="AC16O9_01945"
     CDS             <1..185
                     /locus_tag="AC16O9_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515413.1"
                     /translation="EVEPALHTMRTIAAELRRDALAGLSAAERERFVDTLLTLKANLQ
                     RAQNGAPEGVARRRSR"
     gene            182..1243
                     /locus_tag="AC16O9_01950"
     CDS             182..1243
                     /locus_tag="AC16O9_01950"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6515414.1"
                     /translation="MSAARRARGLLRVVLLLGVPIAAVAAALHYYAEGGRHVETDNAY
                     VKAHIIAVSAEVAGRATEVAVRDNQAVDAGQLLFRIDPAPFEVAVAQADAQLANMRTE
                     VETLRAEHRVALAEAAEAEERIRFLTVQLERQRRLRERGMVREEAYDEARYNLDEARA
                     RLASVRERAARVLAGLGGDPKLPAARHPRVLAAQAARDAAALELARTRVHAPAAGTVS
                     NLRLQAGEHVARGTPVFSLVQAGERWIEANFKETQLTHMRVGQTARVVADAFPGADWQ
                     VRVSAIAPATGAEFALLPPQNATGNWVKVVQRVPVHFALEPADEAERPALRVGMTVSV
                     SVDTGRSRGMPGLLSLFGR"
     gene            1259..2791
                     /locus_tag="AC16O9_01955"
     CDS             1259..2791
                     /locus_tag="AC16O9_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR6515415.1"
                     /translation="MSAQPPVPLSEATGPAAALERALILATCTMVTFLYAMTVTIANV
                     SLPQMQGALSATPDQITWVVTSNLVATAILTPLAGWLVTRFGRRKLCNFAVIGFGLSS
                     LACGLSDSLGELVVFRALQGAFGAPLVPASQAIVLDTYAKRQHGAVIAIFGMGSVLGP
                     IVGPVAGGWLSEAYNWRYVFYMIVPFALLALLGTFAFIHDRASASKARLDWLGFLMLA
                     LALASAQLMLDRGERADWFDSAEIVAWAAIAGIALYAFIVHSATAERPFLDPRTLGDR
                     NFSIGMVLIFVFGMLNFTPMTLLPPMLQGVSGYPDSVIGFVLGARGLGTLVAFFLMIF
                     ASRLDPRAMILLGFLLQAVAGWQLAQLDMNPGTGNVFWPMAIQGFGVGVLWVAITMVT
                     FATLAPERVAEGSAVYHMVRNIGSSIHISISISLAVRMSRANYAELAERVTPYSESLR
                     MPSITGAWNLQDASGQAAFAREMARQAQMIGYLDAFVFFVATSLVVVPLVFLIRLKRP
                     AR"
     gene            complement(2797..3891)
                     /locus_tag="AC16O9_01960"
     CDS             complement(2797..3891)
                     /locus_tag="AC16O9_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6515416.1"
                     /translation="MRITRVEAVPLSIPSRYGADSWALGTGGWRALDFCLVRVDTDAG
                     LTGWGEAFSYSCRRAVVAAVNDMIAPIAVGQDAADIAGLHATIQKRLHIFGRFGITAF
                     ALSGLDIALWDIAGKAAGKPLHALLGGAKRERLECYASLLRYADDKLVARYCGQAASE
                     GFRAIKLHEVDEAVIAAARAAVPREIALLLDVNCEWRVQDAIAVGKRLAPLGLEWFEE
                     PVFPPEDGAGLRAVGEGCGIPIAAGENCCFATQFAALFDARAVQIAQPSVTKVGGVTE
                     LRRVAELAARHQVRLAPHSPYFGPGALATLHLIAALAPDERFEYFYLWADASLYGAAL
                     APIAGGLPVPQAPGLGADPDPEVIRRYRAG"
     gene            complement(3888..4772)
                     /locus_tag="AC16O9_01965"
     CDS             complement(3888..4772)
                     /locus_tag="AC16O9_01965"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515417.1"
                     /translation="MTAKLRLGYVGVGLMGLPMLKRLASLKYAIRAYDIVAERTAAAA
                     AAGAAAASSPADAVRGADLVLLNLPTTDAVEQAVFGEAGVASALKPPQLVVDFSTIKV
                     EKCRAFGAKLRAQTGCGWIDAPVSGGPPASGGGTLTVMAGGEAAHIARAQPLFADVAG
                     RFTHMGPPAAGMATKMLNQLIVGAGHAVMAEAIVLAEAAGIDAARLPECLAGGLADSA
                     LLQKLYPRMQRREFAPQGYVRQLLKDLEMVSEYAAGLKAPTPLMGEALQLYRIVAHLG
                     HTELDTCAVLKVYERESP"
     gene            complement(4776..5645)
                     /locus_tag="AC16O9_01970"
     CDS             complement(4776..5645)
                     /locus_tag="AC16O9_01970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6515418.1"
                     /translation="MSAPFPIVDAHQHFWDPRVNYHPWLCDPEPIAFRYGDYAALRRP
                     YLPADYFADAKGFDVAKTVYIETEWDPRDPLGETEWVERLQRETGFPTVMVAQAWLDR
                     DDAASLLERQATFAFVRGVRHKPRRGQMRDARWCAGYARLARLGLRFDLQAPWSELAD
                     AAELCRAFADTQLILNHTGLPADRSAAGIAGWKRALASLAACPNAAIKISGLGVPGQQ
                     WTPALNRELVLAAIEAFGTGRAMFASNFPVDGLCGGFAEIYSGFREIVKDFPGPEQRR
                     LFHDNALRIYAME"
     gene            complement(5679..6356)
                     /locus_tag="AC16O9_01975"
     CDS             complement(5679..6356)
                     /locus_tag="AC16O9_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6515419.1"
                     /translation="MKAAEIMVKDVVTTGPEASVQELATLMLERRISGLPVVDGSGRL
                     LGIVSEGDLIRRPEIDTDRVKLGWLRLLLSDEARARDFVKSHGRKAREVMTQPAISVA
                     ADAPLAEVVRLMARHRIKRLPVVDKGRLVGLVTRTDLLRAVAARQAVAPADVKDEELR
                     ARIDVMLREEDWAASAVVYVQVEKGVAQLWGTVESQEQREALILAVRGVPGVKDVQPH
                     LGRVLPG"
     gene            complement(6428..7228)
                     /locus_tag="AC16O9_01980"
     CDS             complement(6428..7228)
                     /locus_tag="AC16O9_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6515420.1"
                     /translation="MPSAPLSLNTATVKAQWTLAQAIEGCARHGIRGIAPWRDKLAEM
                     GVKQAASAIKAHGLTVTGLCRGGFFTAGNWKDDNLRAIEEAYALGAQCLVLVVGGLAP
                     GSKDLVHAREKVKEGIATVLPAARQAGVPLAIEPLHPMQAADRACVNTLEQALDLCDA
                     LGDGVGVAVDVYHVWWDPKLEQQILRAGRRRLLAYHICDWLVPTRDLLNDRGMMGDGV
                     IDLPRIAGWVRAAGYGGFHEVEIFSERDWWRRDADEFLAVCKARHRAP"
     gene            complement(7221..8366)
                     /locus_tag="AC16O9_01985"
     CDS             complement(7221..8366)
                     /locus_tag="AC16O9_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6515421.1"
                     /translation="MKIVDVRTHCLASPLETPFAFSQGWVKSRGACLVEVQTDAGFSG
                     WGEALCQGLQPPQIAAAAIESALKPLLIGADPLQLEPLWQRMYMHTRDYGMKGALMAA
                     ISGVDIALWDVAGKHLGQPVWRLLGGAHRERVQIYATGFYRVKGQGEAPRLADEAEQR
                     AAEGFRFLKIKLGFGVADDLAVMQAVARAVEGKGLRMMIDTNHAYGVADAVRLGRALA
                     PLDLRWYEEPVAAEDLAGYRELRAKLEVPIAGGENEFGAWGFRSLLEARAVDIAQPDV
                     CAAGGFTACRHIAALALAHGVQVNPHVWGSSVGQVASLHLACALPLANPSLFADEPVF
                     EYDCSSHPFRETLIDKPLRQREGWLERPEGPGLGIEVDRKALERFRA"
     gene            complement(8363..9679)
                     /locus_tag="AC16O9_01990"
     CDS             complement(8363..9679)
                     /locus_tag="AC16O9_01990"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6515422.1"
                     /translation="MTGAALDPATASLMMFGGFFLLLALRVPVAFALGLACLPVFVIE
                     DRLTPQNLVQETFNAYNSFILLAVPFFLLTANLMNVGGITSRLVRLSRDLVGHFPGGL
                     AQVNVVLSLFFAGISGSSTADAASQSKIFIEAQRKEGYDDSFSVAITAVSAVLAVIVP
                     PSILMIVWGGLLTVSIGGLFLAGIVPGLLIALAQMATVHVYAKLRNYPTYPRATLLET
                     LKAIVIAVPALMTPVIIIGGKIFGWFTATEAACIAVLYAGALTMFVYREMDLKGLHHA
                     LLETGRLTGVALFCVGTASAFGWLLAYYQIPKALLAGVTAWEMGPVATGFFIAAVFLV
                     VGCFLDAIPAIVIVGTVLEPLAKSVGMDPIHFAMIGIVSLAFGLVTPPYGLCLMISAH
                     IAGIRLMDALKDVLIMLLPMFAVLALIIMWPQVVLFLPRLFPPGTL"
     gene            complement(9676..10158)
                     /locus_tag="AC16O9_01995"
     CDS             complement(9676..10158)
                     /locus_tag="AC16O9_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6515423.1"
                     /translation="MLRKLTHWYGTLLSWLLVASVAILVFPVSMQIFSRYTALIPSYI
                     WTEEMARFFFIWSIMLGAMVGIREGTHFVVDLWPPMNARAQAAMRLVASLFVLGFAVV
                     FLWWGIDFTRFAFNRISELAELPLWVIHVAWPLAGATWILFQGEHAWNDLRVLAGRPR
                     "
     gene            complement(10209..11186)
                     /locus_tag="AC16O9_02000"
     CDS             complement(10209..11186)
                     /locus_tag="AC16O9_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6515424.1"
                     /translation="MKRKLFLAVLGAAFAMPAAAQTITLHGASQFNDEHAFTKAMVKF
                     EELVKKYYGKPVNFVLHKNSELGLEKQYFEYMAQGKAVDYAVVSPAHMSTFSKAAPFI
                     DAPFLFRDLDHWNKVLDQDVLKPVADEIAQKADVMLIGFAGGGTRNIFVNKPVRNMEE
                     LKALKVRVQGAPIWSKTFAAAGMAPTVIAYNEVYNAIQNGVIAAGENEAAGVEQMKFY
                     EVAPHLSMTQHAITIRPLCFSGKTFQKLPKDLQAAVLKAGKEAGAYGRKIESSEDEAK
                     LVRMESEGKLKRVPFAGRDQMKRLVDPVMAEYAKDIGADAILAKINAIK"
     gene            complement(11183..12010)
                     /locus_tag="AC16O9_02005"
     CDS             complement(11183..12010)
                     /locus_tag="AC16O9_02005"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6515425.1"
                     /translation="MRLTRLGDGFGAETDDALDAPFQEIWDAFFAAQVLVFRGLRLSA
                     AQFLEFARRFGRPEPHVIDQFHHPEHADILVLSNRKRNGEPVGLADAGTYFHTDYSYL
                     EVPARCTMLYSIEVPAKGGDTLFANQYAAYDDLPEAMKRRIEPLVARHHYGNRDDLDT
                     SSRTVASPLNEAQEQKITWVRHRVVRRHPVTGRKALYAVSGSSFGIEGMPEHEARALL
                     DELKHHATQDKYRLRLRYGVGDLVIWDNASLLHSATLTDPEDPRTLWRITVTEERTS"
     gene            complement(12001..13167)
                     /locus_tag="AC16O9_02010"
     CDS             complement(12001..13167)
                     /locus_tag="AC16O9_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6515426.1"
                     /translation="MKRDAIQTIHLPVAGGGLEPYQVREPRRFDQPGRPFTRVALAAA
                     HVVADPRSAKDPWLDAAIDWDATLAYRRHLWSWGMGIAEAMDTAQRGMGLDWPSSLEL
                     VRRTLEAAKGVEGAVVFSGAGTDHVARADVAGVIGAYEAQCAAIEKLGGRIILMASRA
                     LAASARSPEDYGKVYDHILGQVKQPVIIHWLGEMFDPALEGYWGQKNHEQAMDVCLEV
                     MRRNAAKVDGIKISLLDKDKEIAMRRRLPQGVRMYTGDDFNFAELIEGDAEGHSDALL
                     GIFDSIAPAASAALSALAANDRASYREILAPTVPLSRHIFRAPTRFYKTGVVFMAWLN
                     GHQEHFVMVGGQQSARHILHFAELFRLADAAGLLQDPPLACARMQHLLAVHGCA"
     gene            complement(13164..14312)
                     /locus_tag="AC16O9_02015"
     CDS             complement(13164..14312)
                     /locus_tag="AC16O9_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6515427.1"
                     /translation="MTEKRIGIIMHGVTGRMGMNQHLVRSILEIRKQGGVTLKDGTRL
                     MPDPLLVGRNAAKVEALARQHGVARWTADLDAALASKDDSVFFDAASTQLRPALLKRA
                     IRAGKHIYCEKPVSTTLAEALGLYRAAKKARIKHGVVQDKLWLPGLLKLARLREQGFF
                     GRILSVRGEFGYWVFEGDRQSAQRPSWNYRKEDGGGIILDMLCHWRYVMDNLFGEVQA
                     VSCLGATHIPQRWDENGRKYKATADDAAYATFQLAGGVVAHFNSAWTVRVRRDDLLTL
                     QVDGTQGSAVAGLRECRVQSYDDTPKPIWNPDIPQPIDFFQGWKEVPDANYDNAFKVQ
                     WELYLRHVAGEGAFRWNLLEAAKGVQLAELGLKSWKQKKWLKVPRLKA"
     gene            complement(14309..14761)
                     /locus_tag="AC16O9_02020"
     CDS             complement(14309..14761)
                     /locus_tag="AC16O9_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6515428.1"
                     /translation="MGFRVVSTNHTSFTVSSLDRSIPFFRDCLGFELVSRAPRDPAIV
                     QRITGVAGADMEIAFLRGPGHTVELIEYKAPAAKGRVEARPCDTGFAHLAFNVDDADA
                     AVAAVAAFGVKAIAPPVAINAGPNKGRKVVYVRDWDGVTLEFIETARA"
     gene            complement(14743..16098)
                     /locus_tag="AC16O9_02025"
     CDS             complement(14743..16098)
                     /locus_tag="AC16O9_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515429.1"
                     /translation="MKIAAPRFRVTEVELKERDVRLRMPFRFGVVTLTAAPQAFAHAR
                     IRLEDGREAWGGAAELLAPKWFDKDPALSNEDNFEQLRRSLASARESYLGHEPRTAWA
                     HSAPGRGLVENYGPALIDRALLDALCRALGVSFYRAVQGNLIGAGSFEGIDLGAFFTT
                     LKPRAAVAARHTVGLVDPITAVDQKARVNDGLPETLEEVIAHYRHRHFKLKVAGNVGA
                     DLERLAAIAAVLDRIDGAYYVSLDGNEQYDDIAGVAELWARMKRDARLARLVRSVLFI
                     EQPVTRRKALSARVEGVDKPVIIDESDDALDAFARARALGYRGVSSKTCKGLYKSLVN
                     RARCATWGEGYFMSGEDLTIQPGLALQQDLALVSLLGLTHVERNGHHYVNGMADLPAA
                     EQRAFLAAHPDLYELSHGAVRVKIEDGMLALGSLDAPGYASNAMPEWASMKEMAWASV
                     S"
     gene            complement(16095..>16546)
                     /locus_tag="AC16O9_02030"
     CDS             complement(16095..>16546)
                     /locus_tag="AC16O9_02030"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6515430.1"
                     /translation="PLFHSVIDKATGVAGGRQTFMRITPEHGVIEIGNILGGPSIARP
                     RVATEAFFLAAKYVFEDLGYRRFEWKCNDLNVPSKRAARRFGFAFEGVFRQHMWTKGA
                     NRDTAWFAMLDRDWPRLRAAYERWLAPSNFDAAGRQREKLEVAGPRQ"
BASE COUNT         2462 a         5918 c         5630 g         2536 t
ORIGIN      
        1 aggaggtcga gccggcgctg cacaccatgc gcacgatcgc ggccgagctg cggcgcgacg
       61 cgctggcggg cctcagcgcg gccgagcgcg agcgcttcgt cgacaccctg ctcaccctca
      121 aggcgaacct gcagcgcgcg cagaacggcg cgcccgaggg cgtggcacgc aggcggtccc
      181 gatgagcgcg gcgcgccgtg cgcgcgggct gctgcgcgtc gtgctcctgc tcggcgtgcc
      241 gatcgccgcg gtcgccgccg cgctgcacta ctacgccgag ggcggccgcc acgtggaaac
      301 cgacaacgcc tacgtcaagg cgcacatcat cgccgtcagc gcggaggtcg caggccgcgc
      361 gaccgaggtc gcggtgcgcg acaaccaggc ggtggacgcc gggcagctgc tgtttcgcat
      421 cgatccggcg ccgttcgagg tggcggtggc gcaggccgac gcgcagctcg cgaacatgcg
      481 caccgaggtc gagacgctgc gcgccgagca ccgcgtggcg ctcgcggagg ccgccgaggc
      541 cgaggagcgc atccgctttc tcaccgtgca gctggagcgc cagcggcgcc tgcgcgagcg
      601 cggcatggtg cgcgaggaag cttacgacga ggcgcgctac aacctggacg aagcgcgcgc
      661 gcgcctcgcc tcggtgcgcg agcgcgcggc gcgcgtgctc gcagggctcg gcggcgaccc
      721 gaagctgccg gccgcgcgcc atccgcgcgt gctcgcggcc caggcggcgc gcgacgccgc
      781 ggcgctggag ctggcccgga cgcgcgtcca cgcccccgcc gcggggacgg tgagcaacct
      841 gcggcttcag gccggcgagc acgtggcgcg cggcaccccc gtgttcagcc tggtgcaggc
      901 gggcgagcgc tggatcgagg ccaatttcaa ggagacgcag ctcacgcaca tgcgggtggg
      961 gcagaccgcg cgcgttgtcg cggacgcctt tccgggcgcc gactggcagg tgcgggtgag
     1021 cgcgatcgcg ccggccaccg gcgccgagtt cgcgctgctg ccgccgcaga acgcgaccgg
     1081 caactgggtc aaggtggtgc agcgcgtgcc ggtgcacttc gccctcgagc cggccgacga
     1141 ggccgagcgg ccggcgctgc gcgtcggcat gaccgttagc gtgagcgtcg acacaggccg
     1201 tagccgcggg atgccgggcc tgctgagcct gttcggccgt tagccgcgcc gccggcccgt
     1261 gagcgcgcaa cccccggttc cgctttccga ggccaccggc cccgccgcgg cgctcgagcg
     1321 ggcgctgatc cttgcgacct gcacgatggt gaccttcctc tacgcgatga cggtcaccat
     1381 cgccaacgtc tcgctgccgc aaatgcaggg cgcgctgtcg gcgaccccgg accagatcac
     1441 ctgggtcgtg acctcgaacc tcgtcgccac cgccatcctg acgccgcttg ccggctggct
     1501 ggtcacgcgc tttggccggc gcaagctctg caacttcgcc gtcatcggct tcggcctgtc
     1561 gtcgctggcc tgcgggctgt ccgactcgct cggcgagctg gtggtattcc gcgcgttgca
     1621 gggcgcgttc ggcgcgccgc tggtgccggc gtcccaggcc atcgtgctcg acacctacgc
     1681 caagcgccag cacggtgcgg tgatcgcgat cttcggcatg ggctcggtgc tcggcccgat
     1741 cgtcgggccg gtggccgggg gctggctctc cgaggcctat aactggcgct acgtcttcta
     1801 catgatcgtg ccgttcgcgc tgctggcgct gctcggcacc ttcgccttca tccacgaccg
     1861 cgcgagcgcg agcaaggcgc gcctggactg gctcggcttc ctcatgctcg cgctcgccct
     1921 ggcgagcgcg cagctgatgc tcgaccgcgg cgagcgcgcc gactggttcg attccgccga
     1981 aatcgtcgcc tgggcggcga tcgccggcat cgcgctgtat gcgttcatcg tacacagcgc
     2041 gaccgccgag cggccgttcc tcgacccgcg cacgctcggc gaccgcaact tcagcatcgg
     2101 catggtgctg atcttcgtct tcggcatgct caatttcacg ccgatgacgc tgctgccgcc
     2161 gatgctgcag ggcgtgagcg gctaccccga ttcggtgatc ggcttcgtgc tgggcgcgcg
     2221 cggtctgggg acgctcgtcg ccttcttcct catgatcttc gccagccggc tggatcctcg
     2281 cgccatgatc ctgctcggct tcctgctgca ggccgtggcg ggctggcagc tcgcgcagct
     2341 cgacatgaac cccgggaccg ggaacgtatt ctggccgatg gccattcagg gctttggcgt
     2401 cggcgtgctg tgggtggcga tcaccatggt gaccttcgcc acgctcgccc cggagcgcgt
     2461 cgccgagggg tcggcggtct accacatggt gcgcaacatc ggctccagca tccacatctc
     2521 gatcagcatc agcctggcgg tacgcatgtc gcgcgccaac tacgccgagc tcgccgagcg
     2581 cgtcacgccc tacagcgagt cgctgcgcat gccctcgatc accggcgcgt ggaacctgca
     2641 ggatgcaagc ggccaggcgg cgttcgcgcg cgagatggcg cgccaggcgc agatgatcgg
     2701 ctacctcgac gcgttcgtat tcttcgtcgc gacctcgctc gtcgtggtgc cgctggtgtt
     2761 cctcattcgc ctgaagcggc cggcgcggtg agggcttcag ccggcgcggt agcgccggat
     2821 gacctccgga tccgggtcgg cccccagccc cggcgcctgc ggcaccggca acccacccgc
     2881 aatgggcgcg agcgccgcgc cgtagagcga ggcgtcggcc cacaggtaga agtattcgaa
     2941 gcgctcgtcc ggcgcgagcg ccgcgatgag gtgcagcgtg gcgagcgccc ccgggccgaa
     3001 atatggcgaa tgcggcgcga gccgcacctg gtgcctcgcc gccagttccg ccacgcggcg
     3061 caactccgtg acgccgccca ccttggtgac cgagggctgc gcgatctgca ccgcgcgcgc
     3121 gtcgaacagg gccgcgaact gcgtcgcgaa gcagcagttc tcgcccgccg cgatcgggat
     3181 gccgcagcct tcccccaccg cgcgcaggcc cgcgccgtcc tcgggcggga acaccggctc
     3241 ctcgaaccat tccagcccga gcggcgcgag ccgcttgcct accgcgatgg cgtcctgcac
     3301 gcgccactcg cagttcacgt cgaggagcag cgcgatctcg cgcggcaccg ccgcgcgcgc
     3361 cgccgcgatc accgcctcgt ccacctcgtg cagcttgatc gccctgaagc cttcgcttgc
     3421 ggcctgcccg cagtagcggg cgacgagctt gtcgtcggcg tagcgcagca ggctcgcgta
     3481 gcactcgagc cgctcgcgct ttgcgccacc cagaagcgca tgcagcggct tgcccgccgc
     3541 cttgccggcg atgtcccaca gcgcaatgtc gaggcccgac agagcgaacg cggtgatgcc
     3601 gaagcgcccg aagatgtgca gccgcttctg gatcgtggcg tgcaggccgg cgatgtccgc
     3661 tgcgtcttgc cccaccgcaa tcggcgcgat catgtcgttc actgcggcga cgacggcgcg
     3721 gcggcaggaa taggaaaacg cttcgcccca gccggtgagc cccgcgtcgg tgtccacgcg
     3781 caccaggcaa aaatcgagcg cgcgccagcc gcccgtgccg agcgcccagg agtcggcgcc
     3841 gtaacggctc gggatcgaga gcggcaccgc ctcgacgcgc gtgattctca cggggattcc
     3901 cgctcgtaga ccttgagcac ggcgcaggtg tcgagctcgg tgtgcccgag gtgcgccacg
     3961 atgcggtaga gctgcagcgc ctcgcccatc agcggcgtcg gcgccttcag ccccgcggcg
     4021 tactcgctca ccatctcgag atccttgagc agctggcgca cgtagccctg cggcgcgaac
     4081 tcgcgccgct gcatgcgcgg atagagcttt tgcagcagcg cgctgtcggc cagccccccg
     4141 gccaggcact cgggcagccg cgccgcgtcg atgcccgcgg cttccgcgag cacgatggcc
     4201 tcggccatca ccgcgtggcc cgcgccgacg atgagctggt tgagcatctt ggtcgccatg
     4261 cccgcggccg gcggccccat gtgcgtgaag cgcccggcga cgtcggcaaa gagcggctgc
     4321 gcgcgcgcga tgtgcgcggc ttcaccgccc gccatgacgg tgagcgtgcc gccgccggag
     4381 gccggcggcc cgcccgagac cggcgcatcg atccagccgc agccggtctg cgcgcgcagc
     4441 ttcgcgccga acgcgcggca cttctcgacc ttgatggtgg agaagtcgac cacgagctgc
     4501 ggcggcttga gcgcgctcgc gacgcctgcc tcgccgaaca cggcctgctc gacggcgtcg
     4561 gtggtgggga ggttgagcag cacgagatcc gcgccccgca ctgcgtcggc gggcgaggac
     4621 gcggcagcgg ccccggccgc cgcggccgct gcggtccttt ccgcaacgat gtcgtaggcg
     4681 cggatcgcgt acttgagcga ggcgaggcgc ttgagcatcg gcaggcccat caggcccacg
     4741 ccgacgtatc cgagacgaag tttggcggtc acgtcttact ccatggcgta gatgcgcagt
     4801 gcgttgtcgt ggaacaggcg ccgctgctcc gggccgggaa agtctttcac gatttcccgg
     4861 aacccggaat agatctcggc gaacccgccg cacaggccgt ccacggggaa gttgctcgcg
     4921 aacatcgccc gcccggtgcc gaaggcctct atcgccgcga gcacgagttc gcggttgagc
     4981 gcgggcgtcc attgttgtcc gggcacgccg agccccgaga tcttgatcgc cgcgttcggg
     5041 caggcggcga gcgatgccag ggcccgcttc cagccggcga tcccggcggc gctgcggtca
     5101 gcgggaagcc cggtgtggtt gaggatcaac tgcgtgtcgg caaacgcgcg gcacagctcg
     5161 gccgcgtcgg cgagctcgct ccacggcgcc tgcaggtcga agcgcaggcc gaggcgcgca
     5221 aggcgcgcgt agcctgcgca ccagcgtgcg tcgcgcatct ggccgcggcg cggcttgtgg
     5281 cgcacgccgc gcacgaaagc gaacgttgcc tggcgctcga gcagggacgc cgcatcgtcg
     5341 cggtcgagcc aggcctgcgc caccatgacg gtgggaaaac ccgtctcgcg ctgcaatcgc
     5401 tcgacccact cggtttcgcc gagcggatcg cgcgggtccc actcggtctc gatgtagacc
     5461 gtcttcgcca cgtcgaaacc cttggcgtcg gcgaagtagt cggcggggag gtacggtcgg
     5521 cgcagcgccg cgtaatcgcc gtagcggaac gcaatcggct caggatcgca cagccacggg
     5581 tggtaattga cgcgcgggtc ccagaaatgc tggtgcgcat cgacgatcgg gaacggcgcg
     5641 ctcaccgcgc catcgtagcc gacgggccgc ggcccgtctc agcccggcag cacgcgcccc
     5701 aggtgcggct gcacgtcctt cacgccgggc acgccgcgca ccgcgaggat cagcgcctcg
     5761 cgctgctcct gcgattccac ggtgccccag agctgtgcca cgcccttctc cacttgcacg
     5821 taaacgacgg cgctcgccgc ccagtcctcc tcgcgcagca tgacgtcgat gcgcgcgcgc
     5881 agttcttcgt ccttgacgtc cgccggagcc actgcctggc gcgccgccac cgcgcgcagc
     5941 aggtcggtgc gcgtcaccag gccgacgagc ctgcccttgt ccaccaccgg caggcgcttg
     6001 atgcggtggc gcgccatcag ccgcacgacc tcggcgagcg gtgcgtccgc ggcgacgctg
     6061 atcgcgggct gggtcatcac ttcgcgtgcc ttgcgcccat gcgacttgac gaagtcgcgc
     6121 gcccgcgcct cgtccgacag cagcagccgc agccagccca gcttgacgcg gtcggtgtcg
     6181 atctcgggac gacggatgag gtctccctcg ctcacgatgc cgagcagccg cccgctgccg
     6241 tccaccaccg gcaggccgct gatgcggcgc tcgagcatca gcgtggcgag ctcctgcacc
     6301 gaggcctcgg gaccggtggt gaccacgtcc ttcaccatga tctccgcagc tttcatgcct
     6361 gcacgctacc gggcgcccgg accctcgctt tgatcctggt caagtctcgc tctcctggcc
     6421 gccggactca tggcgcgcga tggcgcgcct tgcacacggc gaggaactcg tcggcgtcgc
     6481 gccgccacca gtcgcgctcg gagaagatct cgacctcgtg aaagcccccg tagccggcgg
     6541 cgcgcaccca gccggcgatg cgcggcaggt cgatgacgcc gtcgcccatc atgccgcggt
     6601 cgttgagcag gtcccgtgtg ggcaccagcc agtcgcagat gtggtacgcg aggaggcgcc
     6661 gccgtccagc gcgcaggatc tgctgctcga gcttcgggtc ccaccacacg tggtagacgt
     6721 ccactgccac gccgacgcca tcgccgagcg cgtcgcacag gtcgagcgcc tgctccaggg
     6781 tgttgacgca ggcgcgatcg gccgcctgca tcgggtgcag cggctcgatg gcgagcggca
     6841 cgccggcctg tcgcgccgcc ggcagcaccg tcgcgatgcc ctccttcacc ttctcgcggg
     6901 cgtgcaccag gtccttcgag cccggcgcca ggccgccgac gaccagcacc aggcactgcg
     6961 cgccgagcgc gtacgcctcc tcgatcgcgc gcaggttgtc gtccttccag ttgccggcgg
     7021 taaagaagcc gccgcggcac agccccgtca ccgtgaggcc gtgcgccttg atcgcgcttg
     7081 ccgcctgctt cacgcccatc tccgcgagct tgtcgcgcca gggggcgatg ccgcggatgc
     7141 cgtggcgcgc gcacccctcg atcgcctgcg ccagcgtcca ttgcgccttc accgtcgcgg
     7201 tgttgagcga cagcggcgcg ctaggcacgg aagcgctcca gcgccttgcg gtccacttcg
     7261 atccccagcc ccggtccctc gggccgttcg agccagccct cgcgctgcct gagcggcttg
     7321 tcgatgagcg tctcgcggaa cgggtgcgag gagcagtcgt attcgaatac cggctcgtcg
     7381 gcgaagagcg acggattggc caggggcagc gcacaggcca gatgcagcga cgccacctgc
     7441 ccgaccgacg acccccacac atgcgggttc acctgcacgc catgcgccag tgcgagcgcg
     7501 gcgatgtgcc ggcacgcggt gaagccgccc gccgcgcata cgtcgggctg cgcgatgtcc
     7561 accgcgcgcg cctccaacag gctcctgaag ccccatgcgc cgaactcgtt ctcgccgccg
     7621 gcgatcggca cctcgagctt cgcgcgcagc tcgcggtagc cggcgaggtc ctcggcggcc
     7681 accggctcct cgtaccagcg caggtcgagc ggcgcgagcg cgcggcccag acgcaccgca
     7741 tcggcgacgc cgtaggcgtg gttggtgtcg atcatcatgc gcaggccctt gccctcgacg
     7801 gcccgcgcca ccgcctgcat taccgccagg tcgtccgcga cgccgaagcc gagcttgatc
     7861 ttgaggaagc gaaaaccttc cgccgcccgc tgctccgcct cgtcggcaag gcgcggcgcc
     7921 tcgccctgtc ccttcaccct gtagaagccc gtggcgtaaa tctgcacgcg ctcgcggtgc
     7981 gcgccgccga gcagccgcca gaccggctgg ccgaggtgct tgcctgcaac gtcccacagc
     8041 gcgatgtcca cgccgctgat cgcggccatc agcgccccct tcatgccgta gtcgcgcgtg
     8101 tgcatgtaca tgcgctgcca cagcggctcg agctgcaggg gatcggcgcc gatcagcagc
     8161 ggcttgagcg ccgactcgat cgccgcggcc gcgatctgcg gcggctgcag gccctggcac
     8221 agcgcctcgc cccagccgct gaagcccgcg tcggtctgca cttcgaccag gcaggcaccg
     8281 cgcgacttca cccagccctg cgagaaggcg aacggcgtct cgagcgggct cgcgaggcag
     8341 tgggtacgga cgtcgacgat cttcacagcg tacccggcgg gaacagccgc ggcagaaaca
     8401 gcaccacctg cggccacatg atgatcagcg ccagcaccgc gaacatcggc agcagcatga
     8461 tgagcacgtc cttcagcgcg tccatgaggc ggatgccggc gatgtgcgcc gagatcatca
     8521 ggcacaggcc gtagggcggc gtcaccaggc cgaaggcaag cgacacgatg ccgatcatgg
     8581 cgaagtggat cggatccatg ccgacgctct tcgccagcgg ctcgagcacc gtgccgacaa
     8641 tcacgatcgc cgggatcgcg tccaggaagc agcccaccac caagaacacc gcggcgatga
     8701 aaaaccccgt cgccaccggg cccatctccc aggcggtcac gcccgcgagc agcgccttcg
     8761 ggatctggta ataggcgagc agccagccga aggccgaagc cgtgccgacg cagaacaagg
     8821 ccacacccgt gagccgcccg gtttcgagca gcgcgtggtg caggcccttc aggtccatct
     8881 cgcggtacac gaacatggtg agcgcaccgg cgtagagcac tgcgatgcag gcggcttccg
     8941 tggcagtgaa ccagccgaag atcttgccgc cgatgatgat caccggcgtc atcagcgccg
     9001 gcacggcgat cacgatcgcc ttcagcgtct ccagcagcgt cgcgcgcggg taggtcgggt
     9061 agttgcgcag cttggcgtag acgtgcacgg tggccatctg cgccagcgcg atgagcaggc
     9121 cgggcacgat gccggcgagg aacagcccgc cgatcgacac cgtgagcagc ccgccccaga
     9181 cgatcatcag gatcgacggc ggcacgatga ccgcgagcac cgcggagacc gccgtgatcg
     9241 ccaccgaaaa gctgtcgtcg tagccttcct tcctctgcgc ctcgataaag atcttcgact
     9301 ggctggccgc atcggccgtg gacgatccgg agatgccggc gaagaacagc gacagcacca
     9361 cgttgacctg cgcgaggcct cccgggaaat gccccaccag atcgcgcgac aggcgcacca
     9421 ggcggctggt gatgccgccc acgttcatca ggttggcggt gaggaggaaa aacggcactg
     9481 cgagcaggat gaacgagttg taggcgttga aggtctcctg caccaaattc tgcggcgtga
     9541 ggcgatcctc gatcacgaac accggcaggc aggccaggcc gagcgcgaac gccaccggca
     9601 cgcgcagtgc gagcagcagg aagaacccgc cgaacatcat caggctggcg gtggcgggat
     9661 cgagcgccgc gccggtcatc gtggccgtcc cgcgagcacg cgcaggtcat tccacgcatg
     9721 ctcgccctgg aacaggatcc aggtcgcgcc ggcgagcggc caggcgacgt ggatcaccca
     9781 cagcggcagc tcggcaagct cggagatgcg attgaaggcg aagcgcgtga agtcgatgcc
     9841 ccaccacagg aacaccaccg cgaaccccag gacgaacagg ctcgccacca ggcgcatcgc
     9901 ggcctgcgcg cgcgcgttca tcggcggcca caggtcgacg acgaagtgcg tgccttcgcg
     9961 gatgccgacc atggcgccga gcatgatcga ccagatgaag aaaaagcgcg ccatctcctc
    10021 ggtccagatg tagctcggga tgagcgcggt gtagcgcgag aagatctgca tcgacaccgg
    10081 aaacaccagg atcgccaccg aggcgacgag cagccaggac agcagcgtcc cgtaccagtg
    10141 cgtgagcttc ctgagcatgg aaaaagcggc ggcgctaccg gcgccgccgc atgtcgaacg
    10201 tcaatgcact acttgatggc gttgatcttg gcgaggatcg cgtcggcgcc gatgtccttg
    10261 gcgtactcgg ccatcaccgg gtcgaccagg cgcttcatct ggtcgcgccc ggcgaacggc
    10321 acgcgcttga gcttgccttc gctttccatg cgcacgagct tcgcctcgtc ctcgctcgac
    10381 tcgatcttgc ggccgtacgc gcccgcctcc ttgcccgcct tcagcaccgc cgcctgcagg
    10441 tccttgggca gcttctggaa cgtcttgccc gagaagcaca gcggccggat ggtgatcgcg
    10501 tgctgcgtca tcgacaggtg cggcgcgacc tcgtagaact tcatctgctc cacgcccgcc
    10561 gcctcgttct cgccggcggc gatgacgccg ttctggatcg cgttgtagac ctcgttgtag
    10621 gcgatcacgg tcggcgccat gcctgccgcg gcgaaggtct tcgaccagat cggcgcgccc
    10681 tgcacccgca ccttgagcgc cttcagctcc tccatgttgc gcaccggctt gttgacgaag
    10741 atgttgcgcg tgccgccgcc ggcgaagccg atcagcatga cgtcggcttt ctgcgcgatc
    10801 tcgtcggcca cgggcttcag cacgtcctgg tcgagcactt tgttccagtg gtcaaggtcg
    10861 cgaaacagga acggcgcgtc gatgaacggt gccgccttgg agaacgtgga catgtgcgcc
    10921 ggcgacacga cggcgtagtc cacggctttg ccctgcgcca tgtactcgaa gtactgcttc
    10981 tccagcccga gctcggaatt cttgtgcagc acgaagttca ccggcttgcc gtagtacttc
    11041 ttcaccagct cctcgaactt caccatcgcc ttggtgaagg catgctcgtc gttgaactgc
    11101 gatgcgccgt gcagcgtaat ggtctgcgcc gccgccggca tggcgaacgc cgcgcctagc
    11161 acggcgagaa acagcttgcg tttcacgatg tcctctcctc tgttacggtt atgcgccaca
    11221 aggtgcgcgg atcttctgga tcggtcagcg tcgccgagtg cagcagcgac gcattgtccc
    11281 agattacgag atcgccgacg ccgtatctca ggcgcagacg gtacttgtcc tgcgtggcgt
    11341 ggtgcttgag ctcgtcgagc agcgcccgcg cctcgtgctc cggcatgccc tcgatgccga
    11401 aggaactccc ggacacggcg tacagcgcct tgcgtccggt gaccgggtgg cgccgcacca
    11461 cgcggtggcg cacccaggtg atcttctgct cctgcgcctc gttcagcggc gaagccacgg
    11521 tgcgcgagga cgtgtcgagg tcgtcgcggt tgccgtagtg gtgccgcgcg accaggggct
    11581 cgatgcgccg cttcatggct tcgggcaggt cgtcgtaggc ggcatactgg ttggcgaaca
    11641 gcgtgtcgcc acccttcgcc ggcacctcga tcgagtacag catcgtgcag cgcgccggca
    11701 cctccaggta cgagtagtcc gtatggaagt aggtgcccgc gtcggccagg cccaccggct
    11761 cgccgttcct cttgcggttg gaaagcacga ggatgtcggc gtgctcgggg tggtggaact
    11821 ggtcgatgac gtgcggctcg gggcgcccga aacggcgcgc gaattcgagg aactgcgcgg
    11881 cgctcagccg cagtccgcgg aagacgagca cctgcgcagc aaagaacgcg tcccagatct
    11941 cctggaacgg cgcgtcgagg gcgtcgtcgg tttccgcccc gaagccgtcg ccgagccgcg
    12001 tcaggcgcat ccgtgcaccg cgagcagatg ctgcatgcgc gcgcaggcga gcggcgggtc
    12061 ctgcagcagc ccggccgcat cggcgaggcg gaacagctcg gcgaagtgca ggatgtggcg
    12121 cgcgctctgc tgcccgccga ccatgacgaa atgctcctgg tggccgttca gccacgccat
    12181 gaacaccacg cccgtcttgt agaagcgcgt cggcgcgcga aagatgtggc gcgagagcgg
    12241 caccgtcggc gccaggatct cgcggtagct cgcgcggtcg ttcgccgcga gcgccgagag
    12301 cgcggccgaa gccgccggcg cgatcgaatc gaagatgccg agcagcgcgt ccgagtgccc
    12361 ttcggcgtcg ccctcgatca gctcggcgaa gttgaagtcg tcccccgtgt acatgcgcac
    12421 gccctgcggc aggcggcggc gcatggcgat ctccttgtcc ttgtcgagga gcgagatctt
    12481 gatgccgtcg accttggcgg cgttcctgcg catcacctcg aggcacacgt ccatcgcctg
    12541 ctcgtggttc ttctgtcccc agtagccttc gagcgccgga tcgaacatct ccccgagcca
    12601 gtggatgatc accggttgct tcacctggcc gaggatgtgg tcgtagacct tgccgtagtc
    12661 ctcgggcgag cgcgcactcg cggcgagcgc gcggcttgcc atcaggatga tgcggccgcc
    12721 gagcttctcg atcgccgcgc actgcgcctc gtacgcgccg atcacgcccg cgacgtcggc
    12781 ccgcgcgacg tgatcggttc ccgccccgga gaacacgacc gcgccctcga cgcccttcgc
    12841 ggcctccagc gtgcgccgca ccagctcgag cgagctcggc cagtcgagac ccatgccgcg
    12901 ctgcgcggtg tccatcgcct cggctatacc catgccccag gaccagaggt ggcgccggta
    12961 ggcgagtgtc gcgtcccaat cgatggcggc gtcaagccac ggatctttcg cggagcgggg
    13021 gtcggcgacc acgtgggccg cggcgagcgc gacgcgcgtg aacggcctgc cgggctggtc
    13081 gaagcgcctc ggctcgcgta cctggtaggg ctcgagcccg ccgccggcga ctggcaggtg
    13141 gatggtctgg atggcgtcgc gcttcatgct ttcagcctcg gcaccttgag ccacttcttc
    13201 tgtttccagc tcttcaggcc aagctcggcg agctgcacgc ccttggctgc ctcgagcagg
    13261 ttccagcgga acgcgccttc gcccgcgacg tggcgcaggt agagctccca ctgcaccttg
    13321 aaggcgttgt catagtttgc gtccggcacc tccttccagc cctggaagaa gtcgatcggc
    13381 tgcgggatgt ccggattcca gatcggcttg ggcgtgtcgt cgtaggactg cacgcggcac
    13441 tcgcgcaggc ccgccacggc gctgccctgc gtgccgtcca cctgcagcgt gagcaggtcg
    13501 tcgcgtcgca cccgcaccgt ccacgccgag ttgaagtgcg cgacgacgcc gcccgcgagc
    13561 tggaacgtgg cgtaggccgc gtcgtccgcg gtggccttgt acttcctgcc gttctcgtcc
    13621 cagcgctgcg gaatatgcgt tgcgcccagg cacgagaccg cctgcacctc gccgaagagg
    13681 ttgtccatca cgtagcgcca gtggcacagc atgtcgagga tgatgccgcc gccgtcctcc
    13741 ttgcggtagt tccacgacgg ccgctgcgcg gactggcgat cgccctcgaa cacccagtag
    13801 ccgaattcgc cgcgcaccga gaggatgcgg ccgaagaagc cctgctcgcg cagccgtgcg
    13861 agcttgagca gtcccgggag ccacagcttg tcctgcacca ccccgtgctt gatccgcgcc
    13921 ttcttcgccg cgcggtacag gcccagcgcc tcggcgagcg tggtcgacac cggcttctcg
    13981 cagtagatgt gcttgccggc gcggatggcg cgcttcagca gcgccggcct gagctgcgtc
    14041 gaggcggcgt cgaagaacac gctgtcgtcc ttggacgcga gcgccgcgtc gaggtccgcg
    14101 gtccagcgcg cgaccccgtg ctggcgcgcc agcgcctcca ccttggccgc gttgcgcccg
    14161 acgagcagcg gatcgggcat cagccgcgtg ccgtccttca gcgtcacgcc gccctgcttg
    14221 cggatctcga ggatcgagcg gaccaggtgc tggttcatgc ccatgcgccc ggtgacgccg
    14281 tgcatgatga tgccgatgcg cttctcggtc atgctctcgc cgtctcgatg aattccaggg
    14341 tcacgccgtc ccagtcgcgc acgtagacca ccttgcggcc cttgttgggg ccggcgttga
    14401 tcgccaccgg cggcgcgatc gccttgacgc cgaacgccgc caccgcggcg accgcggcgt
    14461 cggcgtcgtc gacgttgaac gccaggtgcg caaagcccgt gtcgcagggg cgcgcctcga
    14521 cgcggccctt ggccgcgggc gccttgtatt cgatcagctc caccgtgtgg ccagggccgc
    14581 gcaggaacgc gatctccatg tcggcgcccg cgacgccggt gatcctttgc acgatggccg
    14641 ggtcgcgcgg cgcgcgcgac accagctcga agcccaggca gtcgcgaaag aacggaatcg
    14701 agcggtcgag gctcgacacg gtgaagctgg tgtggttggt gcttacgaca cggaagccca
    14761 tgccatctcc ttcatcgatg cccattccgg catggcgttg ctcgcgtagc cgggcgcgtc
    14821 gagcgagccc agcgccagca tgccgtcctc gatcttcacg cgcaccgcgc cgtggctcag
    14881 ctcgtacagg tcggggtgcg cggcgaggaa cgcccgctgc tccgccgccg gcaggtcggc
    14941 catgccgttc acgtagtggt ggccgttcct ctcgacgtgc gtgaggccga ggagcgacac
    15001 cagcgccagg tcctgctgca gggcgagtcc gggctggatc gtgaggtcct cccccgacat
    15061 gaagtagccc tcgccccacg ttgcgcagcg cgcgcgattg acgagcgact tgtacagacc
    15121 cttgcaggtc ttcgacgaca cgccgcggta gccgagtgcc ctggcgcgcg caaaggcgtc
    15181 cagcgcgtcg tccgactcgt cgatgatcac cggcttgtcg acgccctcga cccgcgccga
    15241 gagcgccttg cgccgcgtca ccggctgctc gatgaacagc acgctgcgca cgagccgcgc
    15301 caggcgcgcg tcgcgcttca tgcgcgccca cagctccgcc acgcccgcga tgtcgtcata
    15361 ctgctcgttg ccgtcgagcg agacgtagta ggcgccgtcg atgcggtcga gcaccgccgc
    15421 gatcgccgcc aggcgctcga ggtcggcgcc gacgtttccc gcgaccttca gcttgaagtg
    15481 gcggtggcgg tagtgcgcga tcacttcttc cagcgtttcc ggcaggccgt cgttcacgcg
    15541 cgccttctgg tcgacagcgg tgattggatc gacgagcccc acggtgtggc gcgcggcgac
    15601 cgcggcgcgc ggcttcagcg tcgtgaagaa ggcgccgagg tcgatgcctt cgaaactgcc
    15661 cgcgccgatc aggttcccct gcaccgcgcg gtagaacgac acgccgagcg cgcggcacag
    15721 cgcgtccagg agcgcgcggt cgataagcgc cggtccgtag ttctcgacca ggccgcggcc
    15781 gggcgcgctg tgcgcccagg cggtgcgcgg ctcgtggccg agatagctct cgcgcgcgct
    15841 ggccagcgag cggcgcagct gctcgaagtt gtcctcgttg gagagcgccg ggtccttgtc
    15901 gaaccacttc ggcgccagca gctcggccgc cccgccccag gcctcgcgcc cgtcctcgag
    15961 ccggatgcgc gcgtgcgcga aagcctgtgg cgccgcggtg agcgtcacca cgccgaagcg
    16021 gaacggcatg cgcaagcgca cgtcgcgctc cttcagctcg acctcggtga cgcgaaagcg
    16081 cggcgcggcg attttcactg gcgtggcccg gcaacctcta gcttctcacg ttggcggccg
    16141 gcggcgtcga aattcgaagg cgccagccag cgctcgtagg cggcgcgcag ccgcggccag
    16201 tcgcggtcga gcatcgcgaa ccaggcggtg tcgcggttgg cgcccttcgt ccacatgtgc
    16261 tggcggaaca caccctcgaa cgcaaagccg aagcggcgcg cagcgcgctt ggagggcacg
    16321 ttgaggtcgt tgcacttcca ttcgaagcgc cgatagccca ggtcctcgaa cacgtacttc
    16381 gccgcgagga agaacgcctc cgtcgcgacg cgcgggcgcg cgatcgacgg gccccccagg
    16441 atgttgccga tctcgatcac gccgtgctcc ggggtgatgc gcatgaaggt ctggcgaccg
    16501 ccggcgacgc cggtggcctt gtcgatcacc gagtggaaca gcggat
//
LOCUS       DBNHKE010000034        11547 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1432954, whole genome shotgun sequence.
ACCESSION   DBNHKE010000034 DBNHKE010000000
VERSION     DBNHKE010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1432954"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="AC16O9_02035"
     CDS             complement(<1..1171)
                     /locus_tag="AC16O9_02035"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6515431.1"
                     /translation="MTGDSPALAQILFVQAWRNERALNVFRVAIWTIVGLITGGAELY
                     ASASVSPGAVLALAWGIGAAVSGLTWLRRYYRRWVAAVLAGIDITVLALCMHAGHRYL
                     LVNDPALVPHQLYASGIVLVVLLATNLLRFSWALSLGAVAYGAAAYFTVLWINDAVDL
                     LTYVELTVIALLGQMLYFSARKLGSIVRQAVERDSLMRFLPAPLLARVSRDPAAVNLE
                     GESQEITALFADIRGFTALAERLGPPAVVQMLNEFFAEMAAEIAAKGGVVMQYIGDNI
                     YAVFPGTGGADHARRALDAALGMLARLEALNDRRAARHEAAIAIGIGLHTGPVVAGSI
                     GSPNLLQYSYVGDTVNTASRIERRTRALDCVLLASGTTLERAGGEAAFAAERMPDE"
     gene            complement(1171..2403)
                     /locus_tag="AC16O9_02040"
     CDS             complement(1171..2403)
                     /locus_tag="AC16O9_02040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6515432.1"
                     /translation="MTDTVRIDLPRAPARRNFLGEAARRIVLSRLERLSQGALALHEG
                     AQTWRFGEPAAPAMEVRVHDPAFYADLAFGGSVGAGEAYMLGHWSAADLTGVMRLLLR
                     NRDALDAMDGGLSRLSAPLRLAAHWLHRNTRAGSRRNIAAHYDLGNEFFRLFLDETMM
                     YSCAIFERPEMTLAEASRAKLDAVCRKLELAPGDHVLEIGTGWGGFALHAAGRYGCRV
                     TTTTISPSQFELARERVRAAGLEDRVTVLLEDYRDLAGRYDKLVSIEMIEAIGHQQFE
                     EFFRRAAARLAPGGTMLLQSIVIDDRHYARARDEVDFIKRYIFPGCCIPSLGALSQAM
                     AAASDLHIEDVEDIGLHYATTLGRWHENFLARLADVRALGYPEQFVRMWEFYLCYCEA
                     GFAERALGDVQLLLRRGA"
     gene            complement(2400..3143)
                     /locus_tag="AC16O9_02045"
     CDS             complement(2400..3143)
                     /locus_tag="AC16O9_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR6515433.1"
                     /translation="MHSALYTGRLRHRRYAPRPHAFDYRLFMAWLDLAELDRVFRGRW
                     FWSTRRPALAWMKRADYLGDAAVPLEDAVRAHVARETGVRPAGPVRLLTHLRMFGHCF
                     NPVSFYYCYDARGERVETVLAEITNTPWNERHAYVLPAAASVGRGRALRFRFDKAFHV
                     SPFLPMGLGYDWRFGTPGARLAVHMENFQGGLKVFDATLALERREITGAALAGALARF
                     PFMTVQVLGAIYWQALRLWARRTPFYAHP"
     gene            complement(3112..4383)
                     /locus_tag="AC16O9_02050"
     CDS             complement(3112..4383)
                     /locus_tag="AC16O9_02050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6515434.1"
                     /translation="MKIAIVGSGIAGNVAAWHLHREHEITLFEAGAHVGGHSHTHEVE
                     QAGRRYAVDTGFIVFNDWTYPNFIRLLGELGVASQPSSMSFSVRDEACGLEYNGTSLN
                     GLFAQRLNLLRPSFLGMLRDILRFNREAPALLEAPGGELPLGELLARGGYGRAFREHY
                     IVPMGAAIWSTDPASMQRFPARFFVRFLHHHGMLSVNARPVWRVIRGGSARYVDKLTA
                     PFRDRIRLRTPVEQVQRLPGSVIVKARGHAAERFDALFVACHSDQALRLLADPSPAER
                     EVLGAIAYQENEAVLHTDTRLLPRRRRAWAAWNYHVRGETDERVALTYNMNILQGLDA
                     PAPFLVTLNRGDAIDPCKVIKRITYHHPQFTPAGVAAQARQAELNGPLNTYYCGAWWR
                     HGFHEDGVVSALAALDHFRERHAQRPVYRSA"
     gene            complement(4380..5264)
                     /locus_tag="AC16O9_02055"
     CDS             complement(4380..5264)
                     /locus_tag="AC16O9_02055"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR6515435.1"
                     /translation="MKTLLLSMRAWLDNDAGVTPGAARGVDWARVIPFIAVHLGCLGV
                     FWVGASTAAVATAVVLFAVRMFAITAFYHRYFSHRAFRTSRLTQFVFALLGASAVQRG
                     PLWWASHHRHHHAHADQPEDAHSARQHGFAWAHAGWFLSRANFATRRELVADLARYPE
                     LRLLDRFDALVPLALAGSLYAVGGLQFLVWGFCISTVALWHATFTINSLAHRYGKRRY
                     ATHDDSRNNLWLALLTFGEGWHNNHHHFPGAARQGFYWWEVDLSWYALRALAALGLVW
                     DLKQVPARVRAARLKGSA"
     gene            complement(5261..5782)
                     /locus_tag="AC16O9_02060"
     CDS             complement(5261..5782)
                     /locus_tag="AC16O9_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR6515436.1"
                     /translation="MRALIALLAGVLVGCANTPTPPLALASQVDLKRFMGDWYVIANI
                     PTFIEKGAHNAVETYRLAADGTIETTFRYRADAFDGEEKQYTARGFVREGTANAVWGM
                     QFIWPFEADYRIAWLAPDYSQVVIGREQRDYVWIMARTPRIPEADYRRILEFLLRQGY
                     DVTRIQRVPQRWS"
     gene            complement(5779..6363)
                     /locus_tag="AC16O9_02065"
     CDS             complement(5779..6363)
                     /locus_tag="AC16O9_02065"
                     /inference="COORDINATES: protein motif:HMM:NF040005.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HIR6515437.1"
                     /translation="MTRTLLIVAAWLLAATAASAQARDWHFRVLLDDAEIGRHRFTLS
                     ENGAGRELRSEARFTVTFLAIPVYRYEHQATETWSDGCLRALDSRTDDDGERLAVNWR
                     APEGGCHMSFAYWNPQILKADRLLNAQTGEMQPVQVQALGEESIEVRGTRVRAQRYRL
                     SAPRLQIDIWHDDGHWVALESLAKGGRRLRYELL"
     gene            complement(6360..7139)
                     /locus_tag="AC16O9_02070"
     CDS             complement(6360..7139)
                     /locus_tag="AC16O9_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6515438.1"
                     /translation="MMTLVYSWLTPAPALLALALIAWAIATVRRNAGLVDIFWPLFFL
                     LGGVVCAALVETLGVRALLVMLLLTAWALRLAAYLALRNWNAPEDHRYRAIRARNEPG
                     FAWKSLYLVFGLQAVLAWLIFAPVAVAIASSTALGWLGGVGAALAAFGVAFEAVADAQ
                     LARFKADAANKGEVMNRGLWRYSRHPNYFGEFCVWWGFYLLALDAGAWWTVFSPVLMT
                     VLLLRVSGVALLERDIGERRPAYRDYVARTNAFFPGAPRNL"
     gene            complement(7136..7663)
                     /locus_tag="AC16O9_02075"
     CDS             complement(7136..7663)
                     /locus_tag="AC16O9_02075"
                     /inference="COORDINATES: protein motif:HMM:NF022537.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HIR6515439.1"
                     /translation="MAFALNIVLFQVGWFACVLGAAHGYAWAGALAALAIVAWHVAQA
                     GDPRREAALVAFAAALGVVFESLLVQSGWIRFGAGVLVEGAATYWMVALWALFATTLN
                     VSLRALRAHAWLAALLGAVGGPLAYYAGARLGALEFVATGAGLAAIGAGWAVLTPLLL
                     RAALRLDGYGAARAA"
     gene            complement(7679..8728)
                     /locus_tag="AC16O9_02080"
     CDS             complement(7679..8728)
                     /locus_tag="AC16O9_02080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6515440.1"
                     /translation="MNGAARLAIGWVEQGLVPDSVVRAGIRRLCEQRLRDIRAGDAAA
                     AARVTEDFVRAMDAADTALVPELANAQHYEVPAEFFGLALGAHRKYSSCWWPEGIASL
                     DDAEAAALATTCRRAGLAPGQDVLELGCGWGSLTLWMAARFPTSRITAVSNSSSQRQY
                     IQGEAARRGLRNVEVLTADMNRFDTDKRFDRVVSVEMFEHMRNWRRLFGRVHGWLRPE
                     GRFFMHVFCHRSTPYAFVEDGPSDWMSRHFFSGGMMPSDELALRFQEALRLVQRWRWD
                     GTHYEKTANAWLANLDARREQALPVLAATYGEADAAQWLQRWRIFFMACAELFGYRDG
                     QEWWVSHYLFERTAR"
     gene            complement(8725..9642)
                     /locus_tag="AC16O9_02085"
     CDS             complement(8725..9642)
                     /locus_tag="AC16O9_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6515441.1"
                     /translation="MQANDGAWLPIRTVAALTGVNAVTLRAWERRYGLCKPARTASGH
                     RLYTHEHVEFIRRVLTLMERGVPISQAQQVLAGEGHKPTSAAQADAAPWRGWRERMAV
                     AVARFDEAALDEVCEAALALHPVERVTSLLFMPVLADLGERWDKVAGGVAEEHFLAVY
                     LRNAIGARFHHRHRLESGPRLLAACVPGEQHEIGLLLFALAAREAGLRIVPLGANVPL
                     ADLARAAQRAACSAIVLSSSIEPRPEVLSRELPALVRAAQVPVFVGGLTAERHRAALV
                     AAGALALSTDIDAGVRVIVHALGATEGAK"
     gene            9790..10275
                     /locus_tag="AC16O9_02090"
     CDS             9790..10275
                     /locus_tag="AC16O9_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6515442.1"
                     /translation="MTLPTPFRTAGAVVRPEWIDYNGHMNVGYYHVAFDVAADVFFEF
                     LGLDPEFRERHGSTTFALEAHLNFLREVKQGDALRFEARLLDYDAKRIHFYMEMFHAG
                     EGFLAASYESLSAHVSVAQRRTAPMPESLLERLAQVKAAHAALPRPWQIGHVMSAKPL
                     R"
     gene            10272..11456
                     /locus_tag="AC16O9_02095"
     CDS             10272..11456
                     /locus_tag="AC16O9_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6515443.1"
                     /translation="MTTVDDAHDAGLLQSVGKLGPAIAAAADEIERLRDIPDALFDRL
                     IEHDLFRLLLPRAYGGAEIDPLLYVRMLEEVAKSDASTAWCLGQNNVCAMVAAYLEPA
                     AAREIFDSPRAILAWGPGPGEARVTDGGYLLSGKFDFASGSRHATWLGAHVPVIEPDG
                     SRRTATDGSARLHTLLFPRSQAQVHDNWRVLGLRGTGSDSYSVTGVRVPESRTLVRDN
                     AVALRQKGPLYVFTQSHLYASGFASIALGIGQACFDAFVGGIRDTVPRGASRSRGANN
                     VVQSEVAQAEARLRSSRMFLRGSLQEIWAGVVRSGAMTDEQRLTIRLASTWAIQQSRE
                     AVTALYLAAGALAIFESQPFERRLRDIHTLCQQMQGHAAHFETVGQMRMGMKLERPLF
                     TF"
BASE COUNT         1854 a         4002 c         4121 g         1570 t
ORIGIN      
        1 gctcgtccgg catgcgttcg gcggcgaacg ccgcctcgcc gccggcgcgc tccaacgtgg
       61 tgcccgaggc gagcagcacg caatcgagcg cccgcgtgcg ccgctcgatg cggctcgccg
      121 tgttgaccgt gtcgcccacg tagctgtact ggagcaggtt gggcgagccg atcgagcccg
      181 ccacgaccgg tcccgtatgc aggccgatgc cgatggcgat tgcggcctca tggcgcgccg
      241 cgcggcggtc gttcagtgcc tcgaggcggg ccagcatgcc gagcgcggca tcgagcgccc
      301 ttcgcgcgtg atccgcgccc ccagtgcccg gaaataccgc gtagatgttg tcgccgatgt
      361 actgcatgac gacgcctccc ttcgcggcga tctcggcggc catctcggcg aagaactcgt
      421 tcagcatctg caccaccgcg ggcggcccga gccgctcggc gagcgcggtg aagccgcgga
      481 tgtccgcgaa cagcgccgtg atctcctgcg actcgccttc cagattcacc gcggccgggt
      541 cgcggctgac gcgtgccagc agcggcgcgg gcaggaagcg catgagcgag tcgcgctcga
      601 cggcctggcg cacgatcgag ccgagcttgc gtgcgctgaa gtacagcatc tggccaagca
      661 gcgcgatcac cgtcagctcg acataggtga gcaggtccac cgcgtcgttg atccagagca
      721 ccgtgaaata cgccgccgcg ccataggcga cggcgccaag cgacagcgcc cacgagaagc
      781 gcagcaggtt ggtagcgagc agcacgacca gcacgatgcc cgaggcgtag agctggtgcg
      841 gcacgagcgc cgggtcgttc accagcaggt aacggtgccc ggcatgcatg cacagcgcga
      901 gcaccgtgat gtcgattccg gccaggaccg ccgcgaccca gcgccggtag tagcgccgca
      961 gccaggtcag gcccgacacc gcggcaccga tgccccaggc gagcgcgagc actgcgcccg
     1021 gggacaccga ggcgctggcg tacagctcgg cgccgccggt gatcaggccg acgatggtcc
     1081 agatggcgac gcgaaagacg ttgagcgccc gctcgttgcg ccacgcctgg acgaacagga
     1141 tctgcgcaag cgcgggcgag tcgcccgtca tcaggctccg cgccgcagca gcagttgcac
     1201 gtcgccgagc gcgcgctccg cgaacccggc ctcgcagtag cacaggtaga actcccacat
     1261 gcgcacgaac tgctcagggt agccgagggc gcgcacgtcg gccaggcgcg cgaggaagtt
     1321 ctcgtgccag cgcccgagcg tcgtcgcgta gtgcagcccg atgtcctcga cgtcctcgat
     1381 gtgcaggtcg ctcgccgccg ccatcgcttg cgacagggcg ccgagcgacg ggatgcagca
     1441 gcccgggaag atgtagcgct tgatgaagtc gacctcgtcg cgcgcgcgcg cgtagtgccg
     1501 gtcgtcgatg acgatcgatt gcaggagcat cgtgccgccg ggcgcgagcc gcgccgccgc
     1561 gcggcggaag aactcctcga attgctgatg gccgatcgcc tcgatcatct cgatcgacac
     1621 cagcttgtcg tagcgcccgg cgagatcgcg atagtcctcg agcagcaccg tgacgcggtc
     1681 ctccagcccc gccgcgcgca cgcgctcgcg cgcgagctcg aactggctcg gcgagatcgt
     1741 ggtcgtggtg acgcggcagc cgtatcggcc ggcggcgtgc agcgcgaagc cgccccagcc
     1801 ggtgccgatc tcgagcacgt gatcgcccgg cgcaagctcg agcttgcggc acacggcgtc
     1861 gagcttggcg cgcgaggctt cggcgagcgt catctccggg cgctcgaaga tcgcgcacga
     1921 gtacatcatc gtttcgtcga ggaacaggcg gaaaaactcg ttgccgaggt cgtagtgcgc
     1981 ggcgatgttg cgccggctgc cggcgcgggt gttgcgatgc agccagtgcg cagcgagcct
     2041 gagcggcgcg gacaggcgcg acaggccgcc gtccatggcg tcgagcgcat cgcgattgcg
     2101 cagcagcagg cgcatcacgc ccgtgagatc cgccgcgctc cagtgcccga gcatgtaggc
     2161 ttcgcccgcg cccaccgagc cgccgaaggc gaggtcggcg tagaacgcgg ggtcgtggac
     2221 gcgcacctcc atggcgggcg cggccggctc gccgaagcgc caggtttgcg cgccttcgtg
     2281 cagcgcgagc gcgccctgcg acaggcgctc gaggcgcgag aggacgatgc ggcgcgcggc
     2341 ctcgccgagg aagttgcggc gcgcgggcgc gcgcggcagg tcgatgcgga cggtatcggt
     2401 catgggtgag cgtagaaagg ggtgcggcgc gcccacaggc gcaatgcctg ccagtagatg
     2461 gcgccgagca cctggacggt catgaaggga aagcgggcga gcgcgccggc gagcgcggcg
     2521 cccgtgatct cgcgacgctc gagcgcgagc gtcgcgtcga acaccttgag cccgccctgg
     2581 aagttctcca tatgcaccgc gagccgcgcg ccgggcgtgc cgaagcgcca gtcgtagccc
     2641 aggcccatgg gcaggaaggg cgacacgtgg aacgccttgt cgaagcgaaa gcgcagcgcg
     2701 cggccgcggc cgacgctcgc ggccgccggc agcacgtagg catggcgctc gttccatggc
     2761 gtgttggtga tctcggcaag cacggtctcg acgcgttcgc cgcgcgcgtc gtagcaatag
     2821 tagaagctca ccgggttgaa gcaatggccg aacatgcgca ggtgcgtgag caggcgcacc
     2881 ggccccgccg gccgcacgcc ggtctcgcgc gcgacgtgtg cgcgcacggc atcttcgagc
     2941 ggcacagccg catcgccaag gtagtccgcg cgcttcatcc aggcgagcgc ggggcgccgc
     3001 gtggaccaga accagcgccc ccggaacacc cggtcgagct cggcgaggtc gagccacgcc
     3061 atgaacaggc ggtagtcgaa cgcgtgcggc cgcggcgcgt agcgccggtg cctaagccga
     3121 ccggtataca gggcgctgtg catgtcgttc gcggaagtga tccagcgcgg cgagcgcgct
     3181 caccaccccg tcctcgtgga agccgtggcg ccaccaggcg ccgcagtagt acgtattcag
     3241 cggtccgttc agctcggcct ggcgcgcctg cgcggcgacg cccgcgggcg tgaactgcgg
     3301 atggtggtag gtgatgcgct tgatgacctt gcaggggtcg atcgcgtcgc cgcggttcag
     3361 cgtgacgagg aacggcgcgg gcgcgtccag cccctgcagg atattcatgt tgtaggtgag
     3421 cgcgacgcgc tcgtccgtct cgccccgcac gtggtagttc caggccgccc aggcgcggcg
     3481 ccggcgcggc agcaggcgcg tatcggtgtg cagcaccgcc tcgttctcct ggtaggcaat
     3541 ggcgcccagc acttcgcgct ccgcggggct cgggtcggcg agcaaccgca gcgcctgatc
     3601 gctgtggcag gcgacgaaca gcgcatcgaa gcgctccgcg gcgtgcccgc gcgccttgac
     3661 gatcacgctg ccgggcaggc gctgcacctg ctccactggc gtgcgcaggc ggatgcggtc
     3721 gcgaaacggc gccgtgagct tgtccacgta gcgcgccgag ccgccgcgaa tcacgcgcca
     3781 cacgggccgc gcgttgaccg agagcatgcc gtggtggtgc aggaagcgca cgaagaagcg
     3841 cgccgggaag cgttgcatcg acgcgggatc ggtggaccag atggcggcac ccatcggcac
     3901 gatgtagtgc tcccgaaagg cgcggccgta gccgccgcgg gcgagcagct cgccgagcgg
     3961 cagctcgccc ccgggcgcct cgagcagcgc cggcgcctcg cggttgaagc gcaggatgtc
     4021 gcgcagcatg ccgagaaacg acggccgcag caggttcagg cgctgcgcaa agagcccgtt
     4081 gagcgaggtg ccgttgtact ccagcccgca cgcctcgtcg cgcacggaga agctcatcga
     4141 gctcggctgc gacgcgacgc cgagctcgcc gagcagccgg atgaagttgg ggtaggtcca
     4201 gtcgttgaac acgatgaagc ccgtgtccac ggcgtaacgc cgtcccgctt gctccacctc
     4261 gtgcgtatgg ctgtggccgc caacatgcgc gccggcttcg aacagcgtga tctcgtgctc
     4321 gcggtgcagg tgccaggccg cgacgttgcc ggcgattcca gagccgacga tggcgatctt
     4381 cacgcggagc cctttagccg ggcggcgcgc acgcgcgccg gcacctgctt caggtcccag
     4441 accaggccga gcgcggcgag cgcgcgcagc gcataccagc tcaggtcgac ctcccaccag
     4501 tagaagccct ggcgcgcggc gccgggaaaa tggtggtggt tgttgtgcca gccctcgccg
     4561 aaggtcagca gcgcgagcca caggttgttg cgcgagtcgt cgtgcgtggc gtagcggcgc
     4621 ttgccatagc ggtgcgcgag cgagttgatg gtgaaggtcg cgtgccaaag cgccaccgtg
     4681 gagatgcaaa agccccacac caggaactgc aggccgccta cggcgtagag gctgcccgcg
     4741 agcgccagcg gcacgagcgc atcgaagcga tcgagcaggc gcagctccgg gtagcgcgcg
     4801 aggtccgcca ccagctcgcg gcgcgtggcg aaattggcgc gcgacaggaa ccagcccgcg
     4861 tgcgcccagg caaagccgtg ctgacgggcc gagtgcgcat cctcgggctg gtcggcatgc
     4921 gcgtggtggt ggcggtggtg cgatgcccac cacagcgggc cgcgctgcac ggcggaggca
     4981 ccgagcagcg cgaacacgaa ctgcgtgagg cgcgaggtgc ggaacgcgcg gtgcgagaag
     5041 tagcgatggt agaacgccgt gatcgcgaac atgcgcaccg caaacagaac gacggcggtc
     5101 gccactgccg cggtgctggc gccgacccag aacacgccga ggcagcccag gtgcaccgcg
     5161 atgaacggga tgacgcgcgc ccagtccacg ccgcgcgcgg cgcccggggt cacgccggcg
     5221 tcgttatcca gccaggcgcg catcgagagg aggagtgttt tcatgaccag cgctgcggca
     5281 ccctctggat gcgcgtcacg tcgtagccct ggcgcaggag gaactcgagg atgcggcggt
     5341 agtccgcctc ggggatgcgc ggcgtgcgcg ccatgatcca cacgtagtcg cgctgctcgc
     5401 gtccgatcac cacctgcgag tagtccggcg caagccacgc gatgcggtag tcggcctcga
     5461 acggccagat gaactgcatg ccccagaccg cgttcgcggt cccttcccgc acgaagccgc
     5521 gcgcggtgta ctgcttttcc tcgccgtcga aagcgtcggc gcgatagcgg aacgtggtct
     5581 cgatggtgcc gtcggcggcg agccggtagg tctcgacggc gttgtgcgcg cccttctcga
     5641 tgaaggtcgg gatattggcg atgacgtacc agtcgcccat gaagcgcttg aggtcgacct
     5701 gcgacgcgag cgcgagcggc ggagtcggcg tgttcgcgca gccgacgagc acgcctgcca
     5761 ggagtgcgat cagggctctc atagcagctc gtagcgcagt cgccgcccgc ccttggcgag
     5821 cgattcgagc gccacccagt ggccgtcgtc gtgccagatg tcaatttgca ggcgcggggc
     5881 gctcaggcgg tagcgctgcg cgcgcacccg cgtgccgcgc acttcgatcg actcttcacc
     5941 cagcgcctgg acctgcaccg gctgcatctc gccggtctgc gcgttgagca aacgatcggc
     6001 cttgaggatc tgcggattcc agtaggcgaa gctcatgtgg cagccgccct cgggcgcgcg
     6061 ccagttcacc gccaggcgct cgccgtcgtc gtcggtgcgc gagtcgaggg cgcgcaggca
     6121 gccgtcgctc caggtctcgg tcgcctggtg ctcgtagcgg tacacgggaa tggcgaggaa
     6181 cgtgaccgtg aagcgcgctt cgctgcgcag ctcgcggccc gcgccgttct cgctcagcgt
     6241 gaagcggtgc cgcccgatct cggcgtcgtc gagcagcacg cggaagtgcc agtcgcgcgc
     6301 ctgcgcgctc gcggcggttg cggcgagaag ccacgcggcg acgatgagca gcgtgcgcgt
     6361 cataggtttc gcggcgctcc ggggaagaac gcgttggtac gggcgacgta gtcgcggtag
     6421 gcgggccggc gctcgccgat gtcgcgctcg aggagcgcca cgcccgatac gcgcagcagc
     6481 agcacggtca tgagcaccgg cgagaacacg gtccaccagg cgccggcgtc gagcgcgagc
     6541 aggtagaagc cccaccagac gcagaactcg ccgaaatagt tcggatggcg gctatagcgc
     6601 cacaggccac ggttcatgac ctcgcccttg ttggcggcgt cggccttgaa gcgcgccagt
     6661 tgcgcgtccg ccactgcctc gaacgccacg ccaaacgccg cgagcgccgc acctaccccg
     6721 cccagccagc cgagcgccgt ggacgaggcg atcgccacgg ccaccggcgc gaagatcagc
     6781 caggccagca cggcctgcag gccaaacacc agatagagac tcttccaggc aaagccgggc
     6841 tcgttgcggg cgcggatggc gcggtagcga tggtcttcgg gggcgttcca gttgcgcagc
     6901 gcgagatagg cggccagccg cagcgcccag gccgtgagga gcagcatcac gagtagcgcg
     6961 cgcaccccaa gcgtttcaac aagcgccgcg cacacgacgc cgccgagcag gaaaaagagc
     7021 ggccagaaga tatcgaccag gcccgcgttg cggcgcacgg tggcgatcgc ccaggcgatg
     7081 agcgcgagcg caagcagcgc cggcgccggc gtgagccagg agtagaccag cgtcatcatg
     7141 ccgccctcgc agcgccgtag ccgtccaggc gcagcgccgc gcgcagcagc agcggcgtga
     7201 gcaccgccca gcccgcgcca atggcggcga ggcccgcgcc ggtggcgacg aattcgagcg
     7261 ccccgagccg cgcgcccgcg tagtaggcga gcggccctcc aaccgcgccg agcagcgcgg
     7321 caagccaggc gtgcgcacgc agcgcgcgca gcgacacgtt cagcgtggtc gcgaacagcg
     7381 cccacagcgc caccatccag taggtggccg cgccttcgac cagcacgccg gcgccgaagc
     7441 ggatccagcc gctttgcacc agcaggcttt cgaacacgac accgagcgcc gcggcgaagg
     7501 ccaccagcgc tgcctcgcgg cgcgggtcgc ccgcctgcgc gacgtgccag gcgacgatcg
     7561 ccagcgccgc gagcgcgccc gcccaggcgt acccgtgcgc cgcgcccagc acgcaggcga
     7621 accagccgac ctgaaacaga acgatgttga gcgcgaacgc catgcgggtc accatcgcct
     7681 agcgcgccgt gcgctcgaac aggtagtggc tgacccacca ctcctgcccg tcgcggtagc
     7741 cgaagagctc ggcgcaggcc atgaagaaga tgcgccagcg ctgcagccat tgggccgcgt
     7801 cggcctcgcc ataggtggcc gccaggacgg gcagcgcctg ctcgcggcgc gcatcgagat
     7861 tggcgagcca ggcgttggcg gttttctcgt aatgcgtgcc gtcccagcgc cagcgctgta
     7921 cgaggcgcag cgcctcctgg aacctgagcg caagctcgtc gctcggcatc atgccgccgg
     7981 agaagaagtg ccggctcatc cagtccgagg ggccgtcctc gacgaacgcg tacggcgtcg
     8041 agcggtggca gaacacgtgc atgaagaaac gcccttcggg ccgcagccag ccgtgcaccc
     8101 ggccgaacag ccgccgccag ttgcgcatgt gctcgaacat ctcgaccgac acgacgcggt
     8161 cgaagcgctt gtcggtgtcg aagcggttca tgtcggcggt gagcacctcg acgttgcgca
     8221 ggccgcggcg cgccgcttcg ccctggatgt actggcgctg cgagctcgag ttggacaccg
     8281 ccgtgatgcg gctggtggga aagcgcgccg ccatccacaa agtgagcgaa ccccagccgc
     8341 agccgagttc gagaacgtcc tggcccgggg cgaggccggc acgccggcag gtggtggcga
     8401 gggccgcggc ctcggcgtcg tccaggctcg cgatgccctc gggccaccag cacgagctgt
     8461 atttgcgatg cgcgccgagg gcgagcccga agaactccgc gggtacctcg tagtgctgcg
     8521 cgttagcgag ctcgggcacg agcgcggtat ccgcggcgtc catggcacgc acgaagtcct
     8581 cggtcacgcg cgccgccgcc gcggcgtcgc ccgcccggat gtcgcgcaag cgctgctcgc
     8641 aaaggcgccg gatgccggcg cgaacgacgc tgtcgggcac caggccctgc tcgacccagc
     8701 cgatcgcaag gcgtgcggcg ccgttcattt cgcgccctcc gtcgcaccga gcgcgtgcac
     8761 gatgacccgc acgccggcgt cgatgtcggt actgagcgcg agcgccccgg cggcgaccag
     8821 cgcagcccgg tggcgctcgg cggtgaggcc gcccacgaac accggcacct gcgcggcgcg
     8881 caccagcgcc ggcagctcgc ggctgagcac ctcgggccgc ggctcgatgg aggacgacag
     8941 cacgatggcg ctgcaggcgg cgcgttgcgc cgcccgcgcc aggtcggcaa gcggcacgtt
     9001 ggcgccaagc ggcacgatgc gcaagcccgc ctcgcgcgcg gcgagcgcaa acaggagcaa
     9061 cccgatctcg tgctgctcgc cgggcacgca ggcggcgagc agccgcgggc ccgattcgag
     9121 gcggtggcga tggtgaaagc gggcaccgat cgcgttgcgc aggtagaccg ccaggaagtg
     9181 ctcctcggcg actccgccgg ccaccttgtc ccagcgctcg ccgaggtcgg cgagcaccgg
     9241 catgaacagg aggctcgtga cccgctccac ggggtgcagc gccagggcgg cctcacagac
     9301 ctcgtccagc gccgcctcgt cgaagcgcgc aacggcgacc gccatgcgct cgcgccaacc
     9361 gcgccaaggg gccgcgtcgg cctgggcggc ggacgtcggc ttgtgcccct cgccggcgag
     9421 cacctgctgc gcctggctaa tgggcacgcc gcgctccatc agcgtgagca cgcggcgaat
     9481 gaactccacg tgctcgtgcg tgtagagccg atggccgctc gcggtacgcg ccggcttgca
     9541 cagcccgtag cgcctttccc aggcgcgcag ggtgacggcg ttgacgcccg tgagcgccgc
     9601 cacggtgcgg atggggagcc aggccccgtc gttggcttgc atcggtgtaa gctgcgcagc
     9661 gttcgttaag atagacgaat cttatacagc aatggctatc ttgtcagtac tttattgtac
     9721 aaatcctgtg cgtcaacgca ccgcaaggct ttctgcggtg atacagggcg atggcactaa
     9781 actgcgcgcg tgacgctccc cacccctttc cgcaccgcgg gcgccgtggt ccgcccggag
     9841 tggatcgact acaacggcca catgaacgtc ggctactacc acgtggcgtt cgacgtcgcg
     9901 gccgacgtgt tcttcgagtt cctggggctg gacccagagt tccgcgagcg ccacggctcg
     9961 acaacgttcg cgctcgaggc gcacctcaac ttcctgcgcg aggtgaagca aggggatgcg
    10021 ctacgcttcg aggcgcggct gctcgactac gacgccaagc gcatccactt ctacatggag
    10081 atgttccacg ccggagaagg cttcctcgcc gcctcgtacg agtcgctcag cgcgcacgtc
    10141 agcgtggcgc agcggcgcac cgcgccgatg cccgaatccc tgctcgagcg cctggcgcag
    10201 gtcaaggcgg cgcatgccgc gctgccgcgc ccctggcaga tcggccacgt catgtcggca
    10261 aagccgctgc gatgaccacc gtggatgatg cgcacgacgc cgggctgctg cagtcggtag
    10321 gcaagctcgg gccggcgatc gccgcggcgg ccgacgagat cgagcgcctg cgcgacattc
    10381 ccgatgcgct gttcgatcgc ctgatcgagc acgatctgtt ccggctgctg ctgccgcgcg
    10441 cgtatggcgg cgccgagatc gacccgctgc tctacgtgcg catgctggaa gaagtcgcga
    10501 agtccgatgc gagcactgcc tggtgcctcg gccagaacaa cgtgtgcgcg atggtggccg
    10561 cgtacctcga gccggcggcg gcgcgggaga tcttcgactc gccgcgcgcg atcctcgcct
    10621 ggggacccgg gcccggtgag gcccgcgtga ccgacggcgg gtacctgctg agcgggaagt
    10681 tcgacttcgc gagcggcagc cgccacgcga cctggcttgg ggcgcacgtg cccgtcatcg
    10741 agcccgacgg ctcgcgccgc acggccaccg acgggtcagc gcggctgcat accctgctct
    10801 tcccgcgctc gcaagcgcag gtgcacgaca actggcgtgt gcttgggctg cgcggcaccg
    10861 gcagcgacag ctatagcgtc acgggcgtgc gggtgcccga atcgaggacc ctcgtgcgcg
    10921 acaatgccgt cgcgctgcga cagaagggtc cgctctatgt gttcacccag agtcacttgt
    10981 acgcctccgg gttcgcttcc atcgcgctgg gcatcggaca ggcctgcttc gacgcgttcg
    11041 tcggcggcat tcgcgacacg gtgccgcgcg gggccagccg ctcgcgcggc gccaacaacg
    11101 tggtgcagag cgaggtcgcg caggccgagg cgcggctgcg ttcctcgcgc atgttcctgc
    11161 gcggctcgct gcaagaaatc tgggccggcg tggtgcgcag cggcgcgatg accgacgagc
    11221 agcggctcac catccgcctg gcgagcactt gggcgatcca gcaatcgcgc gaagccgtta
    11281 ccgcgctcta cttggccgcc ggcgcgctgg cgatcttcga gtcgcagccc ttcgagcgca
    11341 ggctccggga cattcacacg ctctgccagc agatgcaggg ccatgccgcg catttcgaga
    11401 ccgtgggcca gatgcggatg ggcatgaagc tggagcggcc gctctttacg ttctgagcgc
    11461 tacatgctcc tgcggtactg ccctccgacc tcgaacagcg cgtgggtgat ctggccgagc
    11521 gagcacacgc gcacggcgtc catcagg
//
LOCUS       DBNHKE010000035         2821 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1433041, whole genome shotgun sequence.
ACCESSION   DBNHKE010000035 DBNHKE010000000
VERSION     DBNHKE010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1433041"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..322
                     /locus_tag="AC16O9_02100"
     tRNA            233..322
                     /locus_tag="AC16O9_02100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:267..269,aa:Ser,seq:tga)
     gene            375..1631
                     /locus_tag="AC16O9_02105"
     CDS             375..1631
                     /locus_tag="AC16O9_02105"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR6515444.1"
                     /translation="MALIVQKFGGTSVGSVERIRNVAQRVAKWRRAGHDIVVVPSAMA
                     GETNRLLALAAEVQPQPDPRELDVVAATGEQVSAGLLAMALMEAGTSARSYAGWQVAV
                     HTDSAHTKARIVKIDEGRIRKDLAAGRVVVVSGFQGVDGEGNVTTLGRGGSDTSAVAL
                     AAALKAAECQIYTDVDGVYTADPRVVPEARRLHTVTFEEMLEMASAGSKVLQIRSVEF
                     AGKYKVPTRVLSSLTDPILPVEEEALSGTLITFEEDPHMAMEKALVSGVAAQRDEAKI
                     TVHSVPDKPGIAYAILGPVADANINVDVIVQNVSHDGMTDLSFTVSRGDLPRAMKILK
                     DQVKPHIGCKDVTGDDRMAKVSIVGVGMRSHAGIAAQMFRTLAEEGINIQMISTSEIK
                     ISVVVDEKYAELAVRVLHKAFELEKR"
     gene            1659..1752
                     /locus_tag="AC16O9_02110"
     tRNA            1659..1752
                     /locus_tag="AC16O9_02110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1693..1695,aa:Ser,seq:gct)
     gene            complement(1761..2312)
                     /locus_tag="AC16O9_02115"
                     /pseudo
     CDS             complement(1761..2312)
                     /locus_tag="AC16O9_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            complement(2375..2710)
                     /locus_tag="AC16O9_02120"
                     /pseudo
     CDS             complement(2375..2710)
                     /locus_tag="AC16O9_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
BASE COUNT          473 a          925 c          938 g          485 t
ORIGIN      
        1 gcagcaggta ggtgttgcgc aggaccttct gcgcactggc cgataccccc acatcggacg
       61 taaagggcgc gctggcgcgg cccgggctgc gttcgttggt cgggatcggc ttcagttcag
      121 gttgcatccg tttcctccta ggttgctgac agggctaaga ctatcaaaca agcgcgaagt
      181 ttcaacgcat tgcgacgggt aaaatggggt cgcccgcgag cgttttcaat acggaagggt
      241 ggcagagcgg tcgaatgcgc cggtcttgaa aaccggaagg gcctcgcggt cctcgggggt
      301 tcgaatccct ccccttccgc cagacgcaag ctcaggaatt gtctggcttt tcagctagaa
      361 tcgcggtcct ttccatggcg ctcatcgtac agaaatttgg cggcacctcg gtcggctcgg
      421 tcgagcgcat ccgcaacgtg gcgcagcgtg tcgccaagtg gcggcgcgct ggtcacgaca
      481 ttgtcgtggt gccttcggcg atggccggcg agaccaatcg gctgctcgcg ctcgccgccg
      541 aggtgcagcc gcagcccgat ccgcgcgagc tcgacgtggt ggcggccacc ggcgagcagg
      601 tcagcgccgg cctgctggcg atggcgctga tggaagccgg gacttcggcg cgcagctacg
      661 ccggctggca ggtggcggtg cataccgaca gcgcgcacac caaggcgcgc atcgtgaaga
      721 tcgacgaggg ccgcatccgc aaggacctcg ccgcgggccg cgtggtggtg gtgtcgggat
      781 tccagggcgt cgatggcgag ggcaacgtca ccaccctcgg ccgcggcggc tccgacactt
      841 ccgcggtggc gctcgccgcg gcgctcaagg ccgccgagtg ccagatctac accgacgtcg
      901 acggcgtcta caccgccgac ccgcgcgtgg tgcccgaggc gcggcggctg cacaccgtca
      961 ccttcgagga aatgctggag atggcgagcg ccggctccaa ggtgctgcag atccgctcgg
     1021 tggagttcgc cggcaagtac aaagtgccca cgcgcgtgct ttcgagcctc accgatccga
     1081 tactcccggt cgaggaggag gcgctctccg gcacgctcat cactttcgag gaagacccgc
     1141 atatggcgat ggaaaaagcg ctcgtttccg gtgtcgcggc gcagcgcgac gaggccaaga
     1201 tcaccgtgca cagcgtgccc gacaagccgg gcatcgccta cgccatcctc gggcccgtgg
     1261 ccgacgccaa catcaacgtc gacgtcatcg tgcagaacgt gagccacgac ggcatgaccg
     1321 acctgtcgtt caccgtgagc cgcggcgacc tgccgcgggc gatgaagatc ctcaaggacc
     1381 aggtgaagcc gcacatcggc tgcaaggacg tgaccggcga cgaccgcatg gcgaaggtgt
     1441 cgatcgtcgg cgtcggcatg cgctcgcacg ccggcatcgc cgcgcagatg ttccgcacgc
     1501 tggccgagga gggcatcaac atccagatga tctcgacctc ggagatcaaa atctcggtgg
     1561 tagtggacga gaaatacgcc gagctcgccg tgcgcgtgct gcacaaggcg ttcgagctcg
     1621 aaaagcggta gcgcaggcgg ggtacaatcc cctcctgcgg agtgatgccc gagtggccga
     1681 agggggcgcc ctgctaaggc gttatacggg cttaaacccg tatcgagggt tcgaatccct
     1741 ctcactccgc cagtgaatct tcagctcttt tcctgcttca ccagctcttc gagcacctcg
     1801 tagggctgcg cgccgaccac gccccggttg ccgacgacga aggtcggcac gccggtgacc
     1861 ccgtactggt gcgacttccg ccagtccgcg tcgatcgcat ccttgtacgt tcgcttctcg
     1921 agcacctcgc gcgcctggtc ggccggcagg cccaccgacg cggcaatctc gaccagcacc
     1981 tcggggtcgc cgatgttctt cgcatcgacg aagtacgcgc ggaacagcgc gtcgtggatc
     2041 gcctcgccgc ccggctgggt gtcggcccag gcgccgagct cctgcgcgag gcggctgttg
     2101 taggtcatgc tgcgcgtgcc gtagggcagg ccctcagcct gcatgcgcgc gcgcatctgc
     2161 gcctgcatct tcggaatgtc gtagccgcgc ccggcgaaca ggtccgccag gctgcgtcct
     2221 tcctgcggcg tgtccgggtg cagcgggaaa tgcacccact tcacgcgcac gccgtgctct
     2281 ttcctgagtc gctcaatacg cacggtactg aggtagcacc agggtcagac gtagtcggag
     2341 aatatttcca gggtcagggg ttcggtcatg ggcctcattt cttctcagca gcctgcttgt
     2401 cgtagaaatt caccagcctg atcacgtcgg cgggatggat gcggccgatg ggtccggagc
     2461 tgtaggtggc cgcgcgcacc ttgccgtcgg ccgcgaccag gaactcggag ggctggatga
     2521 tgctgcggcg ctcctcccac cacgagccga gctgctcggc catggcgcgc gtgacgccgt
     2581 agcccacggg aaaggacagt tcagcggcga cttccttcgc cttgtcgatg ggatcgacgc
     2641 tcgccgcgac gactttcacg ccgagcccgt cgagctcctt cttgtgctgc tcgaagccgg
     2701 ccaactgccg gcgacagtac ggtcaccagt gaccgcggta gaacaggatg accttgtagc
     2761 gcgcgtccag tcctgcgggc agctcgagct ttccgccgtc gaccaggtcg agcgtcaggc
     2821 g
//
LOCUS       DBNHKE010000036         6764 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1063277, whole genome shotgun sequence.
ACCESSION   DBNHKE010000036 DBNHKE010000000
VERSION     DBNHKE010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1063277"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16O9_02125"
     CDS             complement(<1..220)
                     /locus_tag="AC16O9_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6515445.1"
                     /translation="MSDTAPGSIKVLVIDDSNTIRRSAEMFLRQAGFEVILAEDGFDA
                     LAKISDHQPKVIFVDIMMPRLDGYQTCAL"
     gene            479..1024
                     /gene="grpE"
                     /locus_tag="AC16O9_02130"
     CDS             479..1024
                     /gene="grpE"
                     /locus_tag="AC16O9_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR6515446.1"
                     /translation="MEISADGEPAGRPLDQVLAETRAKLDEQREAWLRALADAENARK
                     RAQSDIAQARKYAAERMVEDLVPVMDSLEAALGAGGADLEALRAGVELTRKQLQSAFE
                     RAGVAEVSPEPGQKFDPHRHQAMSTAESEQEPNTVLAVLQKGYALHDRIVRPALVTVA
                     RAKAVENGPGNPISDSNLESN"
     gene            1035..2987
                     /gene="dnaK"
                     /locus_tag="AC16O9_02135"
     CDS             1035..2987
                     /gene="dnaK"
                     /locus_tag="AC16O9_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR6515447.1"
                     /translation="MAKIIGIDLGTTNSCVALMEGGKPKVIENSEGSRTTPSIVAYTE
                     DGEILVGAPAKRQAVTNAKNTIYAVKRLIGRRFGEKEVEKDIHLMPYKIVKADNGDAW
                     VEVRGKKMAPQQVSAEILRKMKKTAEDYLGEEVSEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKLEKGDRKIAVYDLGGGTFDISIIEIADVEGEKQFE
                     VLSTNGDTFLGGEDFDQRLIDHIVTEFKKDAGVDLSKDVLALQRLKDAAEKAKIELSS
                     SQQTEVNLPYITADASGPKHLTMKVTRAKFESLVDELIQKTIEPCRIAMKDAGLKPGD
                     IADVILVGGQTRMPKVVDKVREFFGKEPRKDVNPDEAVAIGAGIQAGVLKGDVKDVLL
                     LDVTPLSLGIETLGGVMTKLINKNTTIPTKAQQVFSTADDNQTAVTIHVLQGERDMAS
                     GNKSLGQFNLTDIPPAPRGMPQIEVAFDIDANGILHVSAKDKATGKENKIKIQASSGL
                     NEAEIQRMVKDAEAHAEEDRKALELVNARNACEALVHSVKKSLKEYGDKLDAGEKETI
                     EAALKDAEGVLKSEAKAEIEAKAEALAKASQKLGEKMYAESQKAAQAQAAAGQPEGGA
                     QGGEGKKADGDVVDAEFTEVKDKKTG"
     gene            3061..4188
                     /gene="dnaJ"
                     /locus_tag="AC16O9_02140"
     CDS             3061..4188
                     /gene="dnaJ"
                     /locus_tag="AC16O9_02140"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR6515448.1"
                     /translation="MAKRDFYEVLGVNRDASADDIRKAYRKLAMKWHPDRNPDNPKAE
                     DHFKEAQEAYDVLSDAKKRAAYDQFGHAGVDPQAGMGAAGAAGGFTGFGDIFNDIFGD
                     IFGGGRGGRSGVFRGADLRYNLEITLEQAAHGFETRIRIPSMAPCETCQGSGARPGSQ
                     PQTCPTCRGAGQVRVSQGPFSIAQTCPRCHGAGRIIPNPCTTCSGSGRIKQQKTLSVK
                     IPAGVDEGDRVRLSGEGEPGVNGGPAGDLYVQVHIKPHPVFQRDHDDLHCEMPISITT
                     AALGGDIEIPTLDGSARIRVPAESQTGKTFRLRSKGIKGVRSHEAGDLFCHVVVETPV
                     NLTSRQKELLKEFAGISEQDVARHNPRSKSWLEKVKEFFEG"
     gene            4258..5580
                     /locus_tag="AC16O9_02145"
     CDS             4258..5580
                     /locus_tag="AC16O9_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016462.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HIR6515449.1"
                     /translation="MKRTIGRLLLGFTLLCASLNATADAVTDRARALLERKDAVAAYA
                     LLAPLEPQRAGDPEFDYLLGIAALDAGDPERAVFALERVLALQPGNLQARAEIARAYF
                     QIGEHNTAKREFETVRAGSVPAEVRETIDRFLSAIESARSRRFNAYVEVGFGHDTNVN
                     SATAQSQVAVPFFGGAIVQLAPGSTRLKDEFGVLSAGLSFGNDLGRNWSLVGSASYNG
                     KYNNEQSQFDTGTLDGALGLRWSSGSDTVTGAMQLQRYSVDGDSFRESTGGVMQWQHN
                     YSRTSQVSLYAQHAQLKYPDASQSIRDAKRSIVGMATAQALAGRTAPVVFASIYAGEE
                     KEEAQGVPHLGHKPIGVRVGGQWRLGPGVHLSASASMERRRHGGVDPLFFVEREDTQF
                     DASIGLTYTLGSTKWSMRPQLNITDNDSNIDIYAFRRTVAQLSLRRDF"
     gene            5602..>6764
                     /locus_tag="AC16O9_02150"
     CDS             5602..>6764
                     /locus_tag="AC16O9_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6515450.1"
                     /translation="MKPTLFRLCSRGALLAIVSAAFANSAFAATAGRVDFVFGDAALR
                     GSSGQERPLARGVEVMDGDTLITRNGRAQIRFTDGAYVSLQPNTEFGVREYRYDGKAD
                     GTERGFFALLRGAMRTVTGAIGRLNRNAYQVRTPTATVGIRGTGGLIEILPDMATLIR
                     GSSGIWILTNPAGQIDVPAGTSGIAPPDPKEPPKPTDQRPSAPPPPQVSDPTFRQSDE
                     TTASGEACVITATCDSLASPPLVSGPGYHVSFAWGGNTDQWQIGSSGSPTNMTFNGSS
                     QLTAWTDQSGTSSFSGTHVEGGSAAGIVAWGRWIGSATVAPSGQTLTFGSNEGYHYVV
                     GLPTAISSLPTTPGAIPFTLIGATNPTGSDGSFAPGILVSGNMSVDFVQGSVIV"
BASE COUNT         1322 a         2196 c         2234 g         1012 t
ORIGIN      
        1 tgagcgcgca ggtctggtag ccgtcgaggc gcggcatcat gatgtccacg aagatcactt
       61 tcggctggtg atcgctgatc ttcgccagcg catcgaagcc gtcttcggcg agaatcactt
      121 caaagcccgc ctgacgcagg aacatctccg cgctgcgccg gatggtgttg ctgtcatcga
      181 tgaccaggac cttgatcgac cccggcgctg tatcgctcac ggacttcgct ccccctcccc
      241 caacccaaaa tagcgcaatt taaccctttt tacctcaggc tcttatcgca cccacttaat
      301 gtggaatcat acctgtagat tctattccat caagataaaa gacggattta ccgccgcgcc
      361 tggccacttc gggccgaatt tgggcttgaa cgcccgagtc ggcgcccaat atacggagct
      421 ttagagggcc cttttttccc tgatgtccga tccaaagccg caacctgaca aagtgcctat
      481 ggagatctcc gccgacggcg aaccggcggg gcggccgctc gaccaagtgc tcgcggagac
      541 ccgggcgaag ctcgatgagc agcgcgaggc gtggttgcgc gcgctcgccg acgccgagaa
      601 cgcgcgcaag cgcgcgcagt cggacatcgc ccaggcgcgc aagtacgctg ccgagcgcat
      661 ggtcgaggat ctggtgccgg tcatggacag cctggaagct gcgctgggcg cgggcggcgc
      721 ggacctggag gcgctgcgcg ccggggtgga gttgacccgc aagcagctgc agagcgcctt
      781 cgagcgcgcc ggggtcgccg aggtcagccc cgagccgggt cagaagttcg acccccatcg
      841 gcaccaggcc atgtcgacag cggagtcgga gcaggagccc aacaccgtgc tcgcagtcct
      901 gcagaagggc tatgccctgc acgaccgtat cgtgcggccc gccctggtca ccgtcgccag
      961 ggccaaggca gttgaaaacg ggcccggaaa ccccatctct gacagcaact tggaatcgaa
     1021 ctgaaggaac caccatggca aagatcatcg gcatcgacct gggcaccacc aactcctgcg
     1081 tcgccctcat ggagggcggc aagcccaagg tcatcgagaa ctccgagggc tcccggacca
     1141 cgccctcgat cgtcgcctac acggaagacg gcgagatcct ggtcggtgcg ccggccaagc
     1201 gccaggcggt gaccaacgca aagaacacca tttacgcggt gaagcggctc atcggccggc
     1261 gcttcggcga gaaggaggtg gaaaaggata tccacctgat gccctacaag atcgtcaagg
     1321 ccgacaacgg cgacgcctgg gtcgaggtgc gcggcaagaa gatggcgccg cagcaagtgt
     1381 ccgcggagat tctgcgcaag atgaagaaga ctgccgagga ctacctcggc gaggaagtgt
     1441 ctgaggccgt gatcacggtg ccggcgtact tcaacgactc gcagcgccag gccaccaagg
     1501 acgccggccg catcgccggc ctggacgtaa agcgcatcat caacgagccc acggcggcgg
     1561 cgctcgcctt cggcatggac aagctggaaa agggcgaccg caagatcgcc gtgtacgacc
     1621 tgggcggcgg cacgttcgac atctcgatca tcgagatcgc ggacgtcgaa ggcgagaagc
     1681 agttcgaggt gctttccacc aacggcgaca cgttcctcgg cggcgaagac ttcgaccagc
     1741 gcctgatcga ccacatcgtc accgagttca agaaggacgc cggcgtcgac ctgtcgaagg
     1801 acgtgctcgc gctgcagcgc ctgaaggacg cggccgagaa ggccaagatc gagctgtcct
     1861 cgtcgcagca gaccgaggtc aacctgccct acatcacggc cgacgcctcg ggcccgaagc
     1921 acctgacgat gaaggtgacg cgcgccaagt tcgaatcgct ggtggacgaa ctgatccaaa
     1981 agacgatcga gccctgccgc atcgccatga aggacgcggg cctgaagccc ggcgacatcg
     2041 ccgacgtgat cctggtcggc ggccagacgc gcatgcccaa ggtggtcgac aaggtgcgcg
     2101 agttcttcgg caaggagccg aggaaggacg tcaaccccga cgaggcggtg gccatcggcg
     2161 cgggcatcca ggcgggcgtg ctgaagggcg acgtcaagga cgtgctgctg ctcgacgtca
     2221 cgccgctctc gctcggcatc gagacgctgg gcggggtgat gaccaagctg atcaacaaga
     2281 acaccaccat tccgaccaag gcgcagcagg tgttctccac cgcggacgac aaccagaccg
     2341 cggtgaccat ccacgtcctg cagggcgagc gcgacatggc ctcgggcaac aagagcctcg
     2401 ggcagttcaa cctcaccgac attccgcccg cgccgcgcgg catgccgcag atcgaggtgg
     2461 cgttcgacat cgacgccaac ggcatcctgc acgtctcggc caaggacaag gccaccggca
     2521 aggaaaacaa gatcaagatc caagcgagct cgggcctgaa cgaggccgag atccagcgca
     2581 tggtgaaaga cgccgaggcg cacgccgagg aagaccgcaa ggcgctcgag ctggtgaacg
     2641 cgcgcaatgc ctgcgaggcg ctggtgcact cggtgaagaa gtcgctgaag gagtacggcg
     2701 acaagctgga cgccggcgag aaggagacaa tcgaggccgc gctgaaggac gccgagggcg
     2761 tgctcaagtc cgaagccaag gccgagatcg aggccaaggc cgaggcgctg gcgaaggcgt
     2821 cgcagaagct cggcgagaag atgtacgctg aaagccagaa ggcggcacag gcgcaggcgg
     2881 ccgccgggca gccggagggc ggtgcccagg gtggcgaagg caagaaggcg gatggcgacg
     2941 tggtcgacgc cgagttcacc gaggtgaagg acaagaagac gggttgatcg gcaccaccac
     3001 atgacggcag ccgggcggtg cgcgtaggcg cgccgcccgt tttgctgtct gggaaggcac
     3061 atggcgaaac gcgatttcta cgaagtactg ggcgtcaacc gggacgcatc ggcggacgac
     3121 atccgcaagg cgtaccgcaa gctcgccatg aagtggcacc cggaccgcaa tccggacaac
     3181 cccaaggccg aggaccactt caaggaggcg caggaagcct acgacgtgct ctcggacgcg
     3241 aagaagcgcg ccgcctacga ccagttcggc catgccggcg tcgaccccca ggcgggcatg
     3301 ggcgcggcgg gcgccgccgg cggcttcacg ggctttggcg acatcttcaa cgacatcttc
     3361 ggcgacatct tcggcggcgg ccgcggcgga cgctccggcg tatttcgcgg cgccgacttg
     3421 cgctacaacc tcgagatcac gctggagcag gcggcgcacg ggttcgaaac gcgcattcgc
     3481 atcccgtcca tggcgccgtg cgagacctgc cagggcagcg gcgcacggcc cggcagccag
     3541 ccgcagacct gccccacgtg ccgcggtgcc ggtcaggtgc gcgtgtcgca ggggccgttc
     3601 tcgatcgcgc agacctgccc gcgctgtcac ggcgccggca ggatcatccc caatccctgc
     3661 acgacctgca gcggcagcgg gcgcatcaag cagcagaaga ccctgtcggt gaagattccc
     3721 gcgggcgtgg acgagggcga ccgtgtgcgc ctgtccggcg aaggcgagcc cggcgtgaat
     3781 ggcggccccg ccggcgacct gtacgtgcag gtgcacatca agccgcatcc ggtgttccag
     3841 cgcgaccacg acgacctgca ctgcgaaatg ccgatctcca tcaccacggc ggcgctcggc
     3901 ggcgacatcg agattcccac gctggacggg tcggcgcgca tccgcgtacc cgccgagagc
     3961 cagaccggga agaccttccg cctgcgcagc aagggcatca aaggcgtgcg cagccacgag
     4021 gccggggact tgttctgcca cgtcgtggtg gagacgccgg tcaacctgac gtcgcggcag
     4081 aaggaactgc tgaaggagtt cgccggcatc agcgaacagg acgtcgcgcg ccacaatccg
     4141 cgcagcaagt cttggctgga gaaggtgaag gagttcttcg agggctagcc gaggattggc
     4201 gtccctacgg gaccccctgc tagactcgcg gtatcaatcg ccggggaggc aacacccatg
     4261 aagcgcacga tcggccggct cctgctcggt ttcacgcttc tatgcgccag cctcaacgcc
     4321 actgcggatg ccgtgacgga ccgcgcccga gccctgctcg agcgaaagga cgccgtggct
     4381 gcctacgcgc tgctggcgcc gctcgagccc cagcgtgccg gtgacccgga gttcgactat
     4441 ctgctcggca ttgccgcgct ggatgcgggc gatccggagc gggcggtgtt cgcgctggag
     4501 cgcgtgctgg cgctgcaacc ggggaacctg caggcgcgcg cggaaatcgc gcgcgcgtac
     4561 ttccagatcg gcgagcacaa caccgcgaag cgtgaattcg agaccgtgcg cgcggggagc
     4621 gttcctgccg aggtccgcga aacgatcgat cgtttcctat cggcgatcga gtcggcgcgc
     4681 agccggcggt tcaatgcgta cgtcgaggtc ggcttcggcc acgacaccaa tgtgaacagt
     4741 gccacggcgc agtcgcaggt tgccgtgcca ttcttcgggg gtgccatcgt acaacttgcc
     4801 ccgggctcga cgcgcctcaa ggacgaattc ggagtactgt ccgcgggcct ttcttttggc
     4861 aacgacctgg gcaggaattg gtcgctggtg ggcagcgcct cttacaacgg caagtacaac
     4921 aatgaacagt cccagttcga caccggcacg cttgacggcg cgctcggact gcgctggtcc
     4981 agcggcagcg acaccgtgac gggcgccatg caactgcaac gctacagcgt ggacggcgat
     5041 agcttccgcg aaagcaccgg cggcgtcatg cagtggcagc acaactacag ccggaccagc
     5101 caggtatcgc tatacgcgca gcacgcgcaa ctgaagtatc cggacgcctc acagagcatt
     5161 cgcgacgcca agcgcagcat cgtcggtatg gcgacggcac aggctctggc cggccggacc
     5221 gcgcccgtcg tgtttgcgag catctacgcc ggcgaggaga aggaggaggc ccagggcgtc
     5281 ccgcaccttg gccacaagcc gatcggcgtc cgcgtcggcg gccaatggcg actcgggccg
     5341 ggagtgcact tgtccgccag cgcctcgatg gaacggcggc gccatggggg cgtcgacccc
     5401 ctgttcttcg tcgagcgcga agatacccag ttcgacgcct ctatcgggct tacctacacg
     5461 ctcggctcga cgaaatggtc gatgcgtccg cagctgaaca tcacggacaa cgactccaac
     5521 atcgatatct acgcattccg ccgtaccgtc gcgcaactca gcctgcggcg cgatttctag
     5581 ggactcgcca ggagctccgc catgaaaccc actctgttca gactctgttc tcgcggcgca
     5641 ttgttggcga tcgtttcggc ggcattcgcc aattctgcgt tcgccgccac cgccggtcgg
     5701 gtggatttcg ttttcggcga cgcggccttg cgcggctcga gcggccagga gcgcccgctt
     5761 gcgcgtggcg tggaagtcat ggacggcgac acgctgatta cgcgaaacgg ccgcgcacag
     5821 atccgcttca ccgacggcgc ctacgtatcg ctgcagccga acaccgagtt cggcgtgcgc
     5881 gagtaccgct atgacggaaa ggcggacggc actgagcgcg gattcttcgc gctcctgcgc
     5941 ggtgcgatgc gcaccgtcac cggcgctatc ggccgcctca ataggaacgc ctaccaggta
     6001 cgtacaccta ccgccaccgt gggcatccgc ggcaccggcg gcttgatcga gatcctgccg
     6061 gatatggcca cgttgattcg cggctcgagc ggcatctgga ttctgaccaa cccggcgggg
     6121 cagatcgacg tgccggcggg aacttcgggc attgcgccgc ccgatcccaa ggaaccgccc
     6181 aagccgaccg atcagcggcc gtccgcgcca ccgccgccgc aggtgtcgga tccgacgttc
     6241 aggcaaagcg acgagacgac ggcgagcggg gaggcatgcg tcatcaccgc gacgtgcgac
     6301 tcgctggcgt cgccgccatt ggtgtcgggg ccggggtatc acgtgagctt tgcgtggggc
     6361 ggcaataccg atcagtggca aatcggcagt agcggtagcc cgacaaatat gacgtttaac
     6421 gggtcaagtc agctcaccgc ctggaccgat cagtccggca ccagctcgtt ttcagggacc
     6481 cacgtggagg gcggttcggc tgctggcata gtcgcttggg gccggtggat cggctcagca
     6541 accgtcgccc cgtcaggcca gactcttacc ttcggatcca atgagggcta tcactacgtg
     6601 gtgggattgc cgaccgcgat ctcgagcttg ccaaccacgc cgggcgcgat tccgtttact
     6661 ctcatcggcg caaccaatcc gacaggttcc gacggatcat tcgcccctgg aatcctggta
     6721 tccggaaaca tgtcggtgga ttttgttcaa ggttcggtga tcgt
//
LOCUS       DBNHKE010000037         5826 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1433362, whole genome shotgun sequence.
ACCESSION   DBNHKE010000037 DBNHKE010000000
VERSION     DBNHKE010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1433362"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="AC16O9_02155"
     CDS             complement(<1..306)
                     /locus_tag="AC16O9_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6515451.1"
                     /translation="MLKHLKNLFEEPAPRPVPGFERRHLQVAVAALLHEARRADYHQG
                     DDEYAVAQQALAEMFGLGAEQCAAILEEGRVRAEQLTSFYAPVTVIKRDFSLGERIRL
                     "
     gene            367..1179
                     /locus_tag="AC16O9_02160"
     CDS             367..1179
                     /locus_tag="AC16O9_02160"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR6515452.1"
                     /translation="MATYAIGDLQGCADELETLLAQLPFDATRDRLWFVGDLVNRGPK
                     SLAALRRAHALGDAAVVVLGNHDFHLLRLAAGHARNRADDTLDEVLTAPDAPQLIEWL
                     RHRPLMHAEGGYAMVHAGLLPQWSIDKALALAREVQAALRGPRYREFLGALYGAEPRA
                     WRDDLQGWDRLRVIVNAMARMRFCTAEGTMDVAAKGRDAPPGYRPWFELRPAAEPTII
                     CGHWSALGLKLTAKLAALDTGCVWGGSLSALRLEDRTLFQVPCRGYRKPGEA"
     gene            1179..1589
                     /locus_tag="AC16O9_02165"
     CDS             1179..1589
                     /locus_tag="AC16O9_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6515453.1"
                     /translation="MAECIFCKLVAKQIPASIVVEDQATLAFMDLGQVNPGHVLVACK
                     AHVESIYELDEPQAAAVFRTAARVARAVRAAFDPPGLSIYQANGKPAGQTVFHFHLHV
                     LPRHEGDGMQLVWPVKNPPREQLEKYCAQLRARL"
     gene            complement(1586..2524)
                     /locus_tag="AC16O9_02170"
     CDS             complement(1586..2524)
                     /locus_tag="AC16O9_02170"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6515454.1"
                     /translation="MKTLITGSIAYDTIMVFPDRFKNHLLPDQLHILNVCFLTPEMRR
                     EFGGTAGNIAYNLKQLGGDPLVMATVGEDIEPYLYRLQRQGIDAARLKRIPGQFTAQA
                     FITTDLDDNQITAFHPGAMNHAHENRIDAALGAQLAIIAPDGKDGMLQHARECAAARI
                     PFLFDPGQGLPMFAAAELAEFVRLADYVAVNDYEGKLLQEKTGRRLEDLAREVKALVV
                     TLGARGSRIFANGTEHAIPVAQADRVVDPTGCGDAYRAGLLYGIARGWDWPRIGRLGA
                     TLGAIKIASRGAQNHHADRATVEARYRQSFGESPWG"
     gene            complement(2538..3335)
                     /locus_tag="AC16O9_02175"
     CDS             complement(2538..3335)
                     /locus_tag="AC16O9_02175"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HIR6515455.1"
                     /translation="MSALITRCPTCGTTFRALPSQLSVRGGRVRCGKCAAIFDGVANL
                     VEESAAAEAAEPSPQLGLFEAGAPRVPAAARPAPRPAPPRARPTEEEPLPEFLEEEPP
                     RPRYSVAWGLGALIAAAALAGQLAYQYRTEFATLAPPLRPLLAEGCRFLDCEVRLPRR
                     PDLLSIESSDLQSDARRESLVVLNAVIRNRAPFVQELPALELTLTDAADQPLVRRVLR
                     PADYAQGLVAPAPGPGLAPGAEAVLRLHLDTGGVRATGYRLYLFFPA"
     gene            complement(3332..4159)
                     /locus_tag="AC16O9_02180"
     CDS             complement(3332..4159)
                     /locus_tag="AC16O9_02180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6515456.1"
                     /translation="MPWRALTLEVAAAQAETLSDALVEQGAQSVWIGEPGQARLQMHA
                     LFEAGADAPSLLGAAASAAGCPLPLYRTYEVADEDWVRATQAQFKAQCIAGNLWIVPS
                     WEAPPPHAKVVLRLDPGLAFGTGSHPSTRLVLGWLTRTNLTGARVLDYGCGSGILAIA
                     AARLGAAQVDAVDLDPDALDATGDNARANGVAVGAYAPERLPPGDYDIVVANILAQPL
                     IALEPLLGARTRAGGRVALSGILEAQADEVAAAYAGRFATGIAAAEDGWALVEGVRL"
     gene            complement(4159..5520)
                     /gene="accC"
                     /locus_tag="AC16O9_02185"
     CDS             complement(4159..5520)
                     /gene="accC"
                     /locus_tag="AC16O9_02185"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6515457.1"
                     /translation="MFEKILIANRGEIALRVQRACREMGIRTVVVHSTADTEAKYVKL
                     ADESVCIGPAPARESYLNMAAIISAAEVTDAEAIHPGYGFLSENADFAERVERSGFVF
                     IGPHPENIRLMGDKVSAKQAMEKAGVPCVPGSEGALPEDAKEIVRIARKVGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVNMTRSEAAAAFSNPAVYMERYLENPRHIEIQVLADS
                     HKNAITLGERDCSMQRRHQKIIEEAPAPELTEKQRLRIGERCAEACRKIGYRGAGTFE
                     FLYEDGEFFFIEMNTRIQVEHPVTEMITGIDLVQEQIRVAAGEKLRLRQKDVILRGHA
                     IECRINAEDPYKFTPSPGRITSYHPPGGPGIRVDSHVYAGYSVPPNYDSMVGKVIAYG
                     ATRDQAIRRMRIALSEMVVEGIQTNLPLHRELLHDSRFLRGGTSIHYLEQKLAQEHKQ
                     KEK"
     gene            complement(5525..>5826)
                     /gene="accB"
                     /locus_tag="AC16O9_02190"
     CDS             complement(5525..>5826)
                     /gene="accB"
                     /locus_tag="AC16O9_02190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR6515458.1"
                     /translation="APAAPAAAGAPAAASVAEPVAEGHVLKAPMVGTFYRSASPESKA
                     FIEVGQSVKAGETVCIIEAMKLMNEIEADATGVIKAILVENGQPVEYGQPLFIIA"
BASE COUNT          834 a         2003 c         2065 g          924 t
ORIGIN      
        1 caggcggatg cgctctccga gggagaagtc gcgcttgatg accgttaccg gcgcatagaa
       61 gctggtgagc tgctcggcgc gcacgcggcc ctcttcgagg atcgccgcgc actgctcggc
      121 gcccaacccg aacatctcgg cgagcgcctg ctgcgcaacg gcatactcgt cgtcgccctg
      181 atggtagtcg gcgcggcgcg cctcgtgcag cagcgccgcg actgcaacct gcaagtggcg
      241 gcgctcgaag ccgggaaccg ggcgcggcgc cggttcctcg aacaggttct tcaggtgctt
      301 gagcatggcg aacgttagtg tcgcacggcg cggcgcgagt tccgccgcgc ggtagactcg
      361 cgagcgatgg ccacgtacgc gatcggcgac ctgcagggct gtgccgacga gctcgagacg
      421 ctgctcgcgc agttgccctt cgacgccacg cgcgaccggc tctggtttgt cggcgacctc
      481 gtgaaccgcg gcccgaaatc cctcgctgcg ctgcgccgcg cgcatgcgct tggcgatgcc
      541 gccgtcgtcg tcctcggcaa ccacgacttc cacctgctgc ggctggccgc aggacacgcg
      601 cgcaatcgtg ccgacgatac gctggacgaa gtgctcacgg cgccggacgc gccgcagctc
      661 atcgagtggc tgcgccaccg accgctgatg cacgccgagg gcggctacgc catggtgcac
      721 gcaggcctgc tgccgcaatg gtcgatcgac aaagcgctgg cgctcgcgcg tgaagtgcaa
      781 gccgcgctgc gcgggccccg ttaccgcgag ttcctcggcg cgctctatgg cgccgagccg
      841 cgcgcctggc gcgacgatct gcagggctgg gaccggctgc gtgtcatcgt caacgcgatg
      901 gcgcggatgc gcttttgcac ggctgaaggc acgatggacg tcgcagcgaa gggcagggac
      961 gcgccccccg gctaccggcc gtggttcgag ctgcgccccg cggcggagcc gacgatcatt
     1021 tgcgggcact ggtccgcgct cggcctgaag ctcaccgcga agctcgccgc cctcgacacc
     1081 ggctgcgtct ggggcggcag cctttccgcg ctgcgcctgg aagaccggac gctgtttcag
     1141 gtaccctgcc gcggctaccg caaacctggg gaggcatgat ggccgagtgc atattctgca
     1201 agctcgtcgc caagcagatt ccggcgagca tcgtggtcga ggaccaagcc acgctcgcgt
     1261 tcatggatct cggccaggtg aacccgggac acgtgctggt agcgtgcaaa gcgcacgtgg
     1321 agagcatcta cgagctcgac gagccgcagg cggcagccgt gttccgcacg gccgcgcgcg
     1381 tggcccgcgc cgtgcgcgcg gcgttcgatc cgccgggact gtcgatctac caggcgaacg
     1441 gcaagcccgc cgggcagacg gtgttccact tccacctgca cgtgctgccg cgccacgagg
     1501 gcgacggcat gcagctcgtg tggccggtga agaatccgcc gcgcgagcaa ctggagaagt
     1561 actgcgcgca gctgcgcgcc cggctctagc cccagggcga ctcgccgaac gactgccggt
     1621 agcgcgcctc gaccgtggcg cgatcggcgt ggtggttctg cgcgccgcgg ctcgcgatct
     1681 tgatcgcccc gagcgtcgcg cccagcctcc cgattctcgg ccagtcccag cctcgcgcga
     1741 tgccgtagag cagcccggcg cggtaggcgt cgccgcagcc cgtggggtcg acgacgcgat
     1801 ccgcctgcgc caccgggatc gcgtgctcag tcccgttggc gaagatgcgc gagccccgcg
     1861 cgccgagcgt gacgacgagc gccttcacct cgcgtgccag gtcctccagc ctgcgtccgg
     1921 tcttctcctg cagcagcttg ccctcgtagt cgttcaccgc gacgtaatcg gcgaggcgca
     1981 cgaactcggc gagctcggcg gcggcgaaca tcggcaggcc ctggccgggg tcgaacaaga
     2041 acgggatgcg ggccgccgcg cattcgcgcg cgtgctgcag catgccgtcc ttgccgtccg
     2101 gggcgatgat cgcgagttgc gcgccaagcg ccgcgtcgat gcggttctcg tgcgcgtggt
     2161 tcatggcgcc gggatgaaac gccgtgatct ggttgtcgtc gaggtccgtg gtgatgaacg
     2221 cctgcgcggt gaactgcccc gggatgcgct tgagccgcgc cgcatcgatg ccttgtcgct
     2281 gcagccggta caggtagggt tcgatgtcct cgcccaccgt ggccatcacg agcggatcgc
     2341 ccccaagctg cttcaggtta taggcgatgt tgccggcggt gccgccgaac tcgcgccgca
     2401 tttcgggcgt aagaaagcac acgttgagaa tgtgcagctg atcgggcagc aaatgattct
     2461 tgaagcggtc gggaaacacc atgatggtgt cgtaggcgat cgatccggta atcagcgttt
     2521 tcatcgcgct ccgtgcgtca ggcgggaaag aagaggtaca ggcggtagcc ggtggcgcgt
     2581 acgccgcccg tatcgaggtg caggcgcagc acggcttcgg cgccgggcgc gaggcccggc
     2641 ccgggcgcgg gcgcgacgag gccctgcgca tagtccgcgg ggcgtagcac gcggcgcacc
     2701 agcggctgat cggccgcgtc ggtaagcgtg agctccagcg cgggcagctc ctgcacgaat
     2761 ggcgcgcgat tgcggatcac cgcgttcagc acgaccaggc tctcgcggcg cgcatcgctt
     2821 tgcaggtcgg acgattcgat gctgagcagg tccgggcggc gcggcaggcg cacctcgcag
     2881 tccaggaacc ggcagccctc ggcaagcagc ggccgtagcg gcggcgcgag cgtggcgaac
     2941 tcggtgcggt actggtaggc gagctggccg gcaagcgcgg ccgcggcaat cagcgcgccg
     3001 aggccccagg ccacgctgta gcgcgggcgc ggtggctcct cctccaggaa ttcgggcagc
     3061 ggctcttcct cggtcgggcg cgcccgcggc ggcgccgggc gcggggcggg ccgcgctgcg
     3121 gccggcaccc ggggcgcgcc ggcctcgaac aatccgagct gtggcgaggg ctcggccgct
     3181 tcggcggctg cgctctcctc gaccaggttg gcgacgccgt cgaagatcgc tgcgcacttg
     3241 ccgcagcgga ccctgccgcc gcgcaccgag agctgcgagg gcaatgcgcg gaacgtggtg
     3301 ccgcaggtcg gacagcgggt gatcagagcg ctcacaggcg cacgccttcg acgagcgccc
     3361 agccgtcctc tgccgccgcg atacccgtcg cgaagcgccc cgcataggcc gccgcgacct
     3421 cgtcggcctg cgcctccagg atgcccgaga gcgcgacgcg accgcccgcc cgcgtgcggg
     3481 cgccgaggag cggttcaagg gcgatcaggg gctgggcgag gatgttggcc accacgatgt
     3541 cgtaatcgcc gggcggcaat cgctccggcg cgtaggcgcc cacggcgacc ccgttggcgc
     3601 gcgcgttgtc gccagtcgca tcgagggcgt cggggtcgag gtcgacagcg tcgacttgcg
     3661 cggcgccaag ccgtgcggca gcgatcgcca atataccgga accgcatccg taatcgagga
     3721 cgcgcgcgcc cgtgaggttg gtccgcgtta gccagcccag cacgagccgc gtgctggggt
     3781 ggctgccggt gccgaaggca agccccgggt cgagccgcag cacgaccttg gcgtgcggtg
     3841 gcggtgcctc ccaactcggc acgatccaga ggttgccggc aatgcactgc gccttgaact
     3901 gcgcttgcgt cgcccgcacc cagtcttcgt ccgcgacctc gtaggtccgg taaagcggca
     3961 gcgggcaacc ggcggcgctc gctgcggcgc cgagcagcga tggcgcatcg gcgcccgcct
     4021 cgaacagcgc gtgcatctgc agccgggcct ggcccggctc gccgatccat acggattggg
     4081 cgccctgctc caccagcgca tcagacagcg tctcggcctg cgctgccgcg acttccagcg
     4141 tgagcgcgcg ccagggcact atttctcctt ctgcttgtgc tcctgcgcca gcttctgctc
     4201 gagatagtgg atcgaggtgc cgccgcgcag gaaccggctg tcgtgcagca gctcgcggtg
     4261 cagcggcagg ttggtctgga tgccctccac caccatttcc gagagcgcga tgcgcatgcg
     4321 ccggatggcc tgatcgcgcg tcgcaccgta agcgatcacc ttgccgacca tcgagtcgta
     4381 gttgggcggc acgctgtagc cggcgtacac gtgcgagtcg acgcggatgc cgggcccgcc
     4441 gggggggtgg tacgaggtga tgcgcccggg cgagggggtg aacttgtaag gatcctcggc
     4501 attaatgcgg cattcgatgg cgtggccgcg caagatgacg tctttctggc gcaggcgcag
     4561 cttctcgccc gccgcgacgc ggatctgctc ctgcaccagg tcgatgccgg tgatcatctc
     4621 ggtcaccggg tgctcgacct ggatgcgcgt gttcatctcg atgaagaaga actcgccgtc
     4681 ctcgtacagg aactcgaagg tccccgcgcc gcggtaaccg atcttgcggc aggcctcggc
     4741 gcagcgctcg ccgatcctca ggcgctgctt ctcggtgagc tcgggcgccg gtgcctcctc
     4801 gatgatcttc tggtggcgcc gctgcatcga gcagtcgcgc tcgccgaggg tgatggcgtt
     4861 cttgtggctg tcggcgagga cctggatctc gatgtggcgc gggttctcca ggtagcgctc
     4921 catgtacacg gcggggttcg agaacgcggc cgcggcctca ctgcgcgtca tgttgaccgc
     4981 gttgagcagt gccgcctcgg tgtgcaccac gcgcatgccc cgcccgccgc ccccgcctgc
     5041 ggccttgatg atcaccgggt agccgacctt gcgcgcgatg cgcacgatct ccttcgcgtc
     5101 ctcgggcagc gcgccctccg agcctggcac gcacggtacg ccggccttct ccatggcctg
     5161 cttggcgctc accttgtcgc ccatcaggcg gatattctcg gggtgcgggc cgatgaacac
     5221 gaaccccgag cgctcgacgc gctcggcgaa atcggcgttc tcggacagaa agccgtagcc
     5281 ggggtggatg gcctcggcgt ccgtgacctc ggcggcgctg atgatcgctg ccatgttgag
     5341 gtagctctcg cgtgccggcg ccgggccaat gcatacggac tcgtcggcga gctttacgta
     5401 tttcgcttcg gtgtcggccg tggagtgcac gaccacggta cggatgccca tttcgcggca
     5461 ggcgcgctgc acccgcagcg cgatctcccc gcggttggcg atcaggatct tctcgaacac
     5521 cgggctaggc gatgatgaac agcggctgcc cgtactccac cggctggccg ttctcgacca
     5581 ggatggcctt gatcacgccg gtggcgtcgg cctcgatctc gttcatcagc ttcatggcct
     5641 cgatgatgca gaccgtctcg ccggccttga cgctctggcc gacctcgata aaggctttcg
     5701 attccggcga cgccgagcgg tagaaggtcc ccaccatcgg cgccttaagg acgtggcctt
     5761 cagcgaccgg ctccgcgacc gaagcggcgg cgggtgcgcc cgccgccgcg ggggcggcgg
     5821 gggcag
//
LOCUS       DBNHKE010000038         8695 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_216802, whole genome shotgun sequence.
ACCESSION   DBNHKE010000038 DBNHKE010000000
VERSION     DBNHKE010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_216802"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC16O9_02195"
     CDS             complement(<1..451)
                     /locus_tag="AC16O9_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6515459.1"
                     /translation="MLTPERRAAYTRAELERLVAPRSIAVVGASPRAGSFGVRTLENL
                     AQFAGEVFPVNAKYEMIGERRCYASLASLPRSPDCVVLAVPREGVEAALEEAAAAGAG
                     SAIVFASGYGEMTREDAARAQHRLGEIARAAKMPMLGPNCMGMVNHAA"
     gene            482..1258
                     /locus_tag="AC16O9_02200"
     CDS             482..1258
                     /locus_tag="AC16O9_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6515460.1"
                     /translation="MNVKQLLDLKGRVALVTGGSRGLGLQMAEALGEMGARLALTARK
                     QAELDEAVAHLKAQGIEATAWVCDLGKREAIAPVVEAILAKLSRIDILVNNAGATWGA
                     PAEDHPLEAWDKLVNLNLTGTFLASQLVAKKSMIPSKWGRIVNVASIAGIAASDPRVV
                     KTVAYNATKHGVVGLTRQLAAEWGEHGITVNAICPGFFPSRMTKATLDTLGRMVAELT
                     PTKRLGNDEDLKGLVVLLASEASRHITGQAIAVDGGAILI"
     gene            1255..1680
                     /locus_tag="AC16O9_02205"
     CDS             1255..1680
                     /locus_tag="AC16O9_02205"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6515461.1"
                     /translation="MSADATLQFTRDYQNRIPFVAHLGLTTDALGAGTARLSMPLPAH
                     FTNSLGTAHGGVIMSLLDVALCTAARTLHPESTGVVTIDMTTSFIGGGKGHQLIAEAR
                     VLKDGRSITFVEGEAKNADGSLVAKAIGTVRVRHKEKET"
     gene            1694..2470
                     /locus_tag="AC16O9_02210"
     CDS             1694..2470
                     /locus_tag="AC16O9_02210"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR6515462.1"
                     /translation="MTANPYSAPAAAVADPVAPQGKFIPGGRRVAAGHGWSWIAQGWD
                     LFKRQPGLWIGISVTLFVILLVLALIPVLGPFANYLLWPVLSAGLLLGCGALVEGGNL
                     EFNHLFAGFRGRFGTLIAVGAISFGISLAIAVLVAVVMGVGIGTMLGGGPGPNAAPEV
                     LMTVLLAMLVMFALLLPLVMAMWFAPALVVFHERGAVEAMKESFAGCLKNVLPFLIYG
                     LIALAFAILAAIPFGLGWLLLGPVLIASVYASYRDIYLEA"
     gene            complement(2455..3666)
                     /locus_tag="AC16O9_02215"
     CDS             complement(2455..3666)
                     /locus_tag="AC16O9_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515463.1"
                     /translation="MRVTLPFLAGYFISYLYRSVNAIIGPVIARELGLTAAGLGLLTA
                     IFFFAFAVFQLPLGVLLDRYGPRRVNATMLLVAAVGGIWFASGSSAAELTAARGLIGL
                     GVSGCLMAAFSAFALWYPSERLASMNGIAFASGMLGAVVATVPLEMALRVFHWREVFA
                     GIVALNLAVSLLLYLVVPERSATQRGESVGAQLRGFGAIARDPAFWRVALCIGASQVA
                     AVSLSTLWVATWLRDIAGYTAAEVARALLLFSLAMMLGYVGFGRAADARVRSGRSALP
                     LLAGGVAAASVALAMLALGVRAGALALWCVFFAGSTAVVLSYALFSRRYPRHMVGRVN
                     TALNVFVFIGMFSGQAAVGLVLSLWPQTAAGYAPDAYAWALGLLWLTQFAGLAWLWSG
                     RRLLAADQASR"
     gene            complement(3675..4766)
                     /locus_tag="AC16O9_02220"
     CDS             complement(3675..4766)
                     /locus_tag="AC16O9_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6515464.1"
                     /translation="MAEDRKESRLLARLLRVQLFPRRGSGRRVRLLVRQEESTAARTL
                     ALRVVLVLVLFAAVLAVFWLDRDGLRDASDGVVSFLDVVYFTMVTVTTVGYGDIVPVT
                     DRARLIDSFFVTPIRFFVLFIFVGTAYQMVFQRMMEERRMRKLEDSLSDHVIVCGFGH
                     SGRIAAVELVAKGEKPEQVVVIDQSEGAARAAAEAGFVALVGSCTSEEIMRRAVVQQA
                     RALLLCIGRDDTAVLATLTARHLSDKVRIIASVRENENAKLLRQGGADVIVSPARVGG
                     YLLADAVESAYTVGLINELLTERGRVRLTERAPNPDEVGREARALPCLVIGIERDKKR
                     ILFWDLGDTTVRADDLMLVIEGPATEARA"
     gene            complement(4793..5566)
                     /locus_tag="AC16O9_02225"
     CDS             complement(4793..5566)
                     /locus_tag="AC16O9_02225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6515465.1"
                     /translation="MPSTELKGKVAWITGGGSGIGLAGAKALAAAGAHVIISGRTART
                     NESGLAELKKLGSAEANLLDVGERAAVEKAAAEIEKRHGRIDILVTSAGTNIGGGKRN
                     LKTMSLEGWDDVVKINLNGLFYCCYAVLPGMRQRKDGVIINISSWAGRYASVLTGPAY
                     NATKRAVIAVSESINMEECTNGIRATSILPGEVATPIMEKRPVPPSAEERARMAQAED
                     FGKAILFVATMPARTCVNEIIMAPTWNRFYVGGIEPPPR"
     gene            complement(5553..6245)
                     /locus_tag="AC16O9_02230"
     CDS             complement(5553..6245)
                     /locus_tag="AC16O9_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HIR6515466.1"
                     /translation="MIDLYTWPTPNGHKIHIMLEETGLPYKVIPVDIGAGDQFKPEFL
                     KISPNNKMPAMVDSDGPDGKPMALAESGAMLFYLASKTGRFLPEDIRKRWQVMQWVMF
                     QMGHIGPMLGQAHHFLGYAPERIEYAMNRYKNEANRLYGVVDRRLKESKYLACDEYTI
                     ADMATMPWMRFPERQGVNIEEYPNVRRWRDGIAARPAVERALKVLADRRRAEMTPEQK
                     ENLFGAKQYAKH"
     gene            complement(6242..7240)
                     /locus_tag="AC16O9_02235"
     CDS             complement(6242..7240)
                     /locus_tag="AC16O9_02235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6515467.1"
                     /translation="MNKRIVLASYPEGWVTEANFRLETAPVPKPGEGEVLVKNHWLSL
                     DPYMRGRMSQQKSYVKGVEPGEVMTGETAGEVVESKDPRFKPGDKVATGQGGGWQLYS
                     CLKGERLAKVDDAKAPLSYYLGCLGMPGRTAYFGMKDICQPKPGETVVVSAASGAVGS
                     VVGQLAKAWGCRAVGIAGGKAKCDYVVKELGFDACVDYKAGDVYAQLKAACPKGIDAD
                     FENVGGEILDTVLRLMNVRGRIAVCGLISDYNAKEPYGVKMIRSVLVNRLTVRGMIVF
                     DWQPRYPEANQALLDLVAAGKLKYRESVVQGIENAPKGLIGLLKGENFGKQLVKLT"
     gene            complement(7268..8485)
                     /locus_tag="AC16O9_02240"
     CDS             complement(7268..8485)
                     /locus_tag="AC16O9_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6515468.1"
                     /translation="MAVEFRADAPEFLRDPFPLYRALRAEDPVHWSPRLKAWVLTRYD
                     DCRAVLLDREMSSDRLRPFFATLPGPEAERIGDIMRYLTHWMVFRDPPEHTRLRRLTS
                     KVFHLQSMNAMRPQVEALAAWLLGRLGDREKFDFIADFAGPLPGLVIMAMLGVPRDHL
                     PVIKRLSDDMALFIGSSRMSPEKYAIAEAATREMAAFFRGLIAERRAAPRADLLSQLV
                     HLEDDGDRLSEDELVATCILLLFAGHETTTNHIANAMLALLRFPGEAAALRADPALAP
                     RAVEELLRYDGPSGAQVRVVKVAQEMRGKRLAPGDRVFLMLNAANRDPEAYDDPERLD
                     LRRKEVPHLTFGFGLHICLGFPLARMEGQVALPAVLRHWKRIELAQPEERLEWLNSMV
                     FRGMAALPLRVVR"
     gene            complement(8476..>8695)
                     /locus_tag="AC16O9_02245"
     CDS             complement(8476..>8695)
                     /locus_tag="AC16O9_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515469.1"
                     /translation="RGGGEARRRVHGGAAPAGPVVILDIQPRGPAFCSGGDLAKMEKA
                     AKMTKARASAEAREGVRSFLEKRKPRWQ"
BASE COUNT         1323 a         3082 c         2971 g         1319 t
ORIGIN      
        1 gcgccgcgtg attgaccatg cccatgcagt tgggcccgag catcggcatc ttcgcggcgc
       61 gcgcgatctc gcccaggcgg tgctgcgcgc gcgcggcgtc ctcgcgcgtc atctcgccgt
      121 acccggaggc gaagacgatc gccgagcccg cgcccgccgc ggcggcctcc tccagcgcgg
      181 cctccacgcc ctcgcgcggc accgccagca ccacgcagtc gggcgagcgc ggcagcgacg
      241 cgagcgaggc gtagcagcgc cgctcgccga tcatttcgta cttggcgttc accgggaaca
      301 cctcgcccgc gaactgcgcg aggttctcga gcgtgcgcac gccgaaggag ccggcgcgcg
      361 gcgaagcgcc gaccacggcg atcgagcgcg gcgcgacgag gcgctcgagc tcggcgcgcg
      421 tgtacgccgc gcggcgctcc ggtgttagca taggcgaaac gataacagca tcggcctttg
      481 catgaacgtc aagcagcttc tggatctcaa ggggcgcgtc gcgctggtca ccggcggctc
      541 gcgcgggctc ggcctgcaga tggccgaggc gctcggcgag atgggcgcgc gcctcgcgct
      601 cacggcgcga aagcaggccg agctcgacga agcggtcgcg cacctgaagg cgcagggcat
      661 cgaggccacg gcctgggtct gcgacctcgg caagcgcgag gcgatcgccc cggtggtcga
      721 ggcgatcctc gccaagctca gcaggatcga catcctcgtc aacaatgccg gcgccacctg
      781 gggcgcgccg gccgaggatc atccgctgga agcctgggac aagctcgtga acctcaacct
      841 cacgggcacc ttccttgcct cgcagctcgt cgccaagaaa tccatgatcc cgtcgaaatg
      901 gggccgcatc gtcaacgtcg cctcgatcgc cggcatcgcg gcgagcgatc cgcgcgtggt
      961 gaagaccgtg gcctacaacg cgaccaagca cggcgtcgtg ggcctcacgc ggcagctggc
     1021 ggcggagtgg ggcgagcacg gcatcaccgt gaacgccatc tgcccggggt tcttcccctc
     1081 caggatgacc aaggcgacgc tcgatacgct cgggcgcatg gtggcggagc tcacgcccac
     1141 caagcgcctc ggcaacgacg aggacctgaa gggcctcgtc gtcctgctcg cctcggaggc
     1201 ctcgcgccac atcaccgggc aggcgatcgc ggtggacggc ggcgcgatcc tgatatgagc
     1261 gccgacgcga cgctgcaatt cacgcgcgac taccagaacc gcatcccgtt cgtcgcgcac
     1321 ctgggcctca ccaccgacgc gctcggcgcg ggcacggcgc gcctgtcgat gccgctgccc
     1381 gcgcacttca ccaacagcct cggcacggcg cacggcggcg tcatcatgtc gctgctcgac
     1441 gtggcgctgt gcaccgcggc gcgcacgctg cacccggagt ccaccggcgt ggtcaccatc
     1501 gacatgacca cgtccttcat cggcggcggc aagggccatc agctgatcgc cgaggcgcgc
     1561 gtgctgaagg acgggcgcag catcaccttc gtggagggcg aggcgaagaa cgccgacggc
     1621 tcgctcgtcg ccaaggcgat cggcacggtg cgcgtgcgcc acaaggagaa ggaaacctga
     1681 gaggaggccg gacatgaccg ccaatcccta ctccgcccct gccgcggcgg tcgctgatcc
     1741 ggtcgccccg cagggcaagt tcattccagg cggccgccgc gtcgccgctg gccacggctg
     1801 gagctggatt gcgcagggct gggacctgtt caagcgccag cctggcctgt ggatcggcat
     1861 ttcggtgacg ctgttcgtga tcttgctggt gctggcgttg atccccgtgc tcggcccgtt
     1921 cgccaactat ctgctgtggc cggtgctgag cgccgggctg ctccttggct gcggcgcgct
     1981 cgtcgaaggc ggcaacctgg agttcaacca tcttttcgct gggtttcgcg ggcgcttcgg
     2041 cacgctgatc gcggtaggcg ccatctcctt cggcatttcc ttggccatcg ccgttctcgt
     2101 cgcggtcgtc atgggcgtgg gcatcggcac gatgctgggt ggcggccccg gccccaacgc
     2161 cgcgccggaa gtgctgatga cggtgctgct cgccatgctg gtcatgttcg cgctgctgct
     2221 gccgctggtg atggcgatgt ggttcgcgcc ggcgctggtg gtgttccacg agcgcggggc
     2281 ggtcgaagcc atgaaggaga gcttcgccgg ctgcctgaag aacgtcctgc cattcctgat
     2341 ctacggcctg atcgcgctgg cgttcgcgat cctcgcggcc atccccttcg gcctggggtg
     2401 gctgctgctc ggcccggtgc tgatcgcctc ggtgtacgcg tcctaccgcg acatctacct
     2461 cgaggcctga tcggccgcca gcagccggcg gcccgaccac agccaggcca ggcccgcgaa
     2521 ctgcgtgagc cacagcagcc ccagcgccca cgcgtaggcg tcgggcgcat agcctgccgc
     2581 cgtctgcggc cacaggctga gcaccagccc cacggccgcc tggccgctga acatgccgat
     2641 gaagacaaag acgttgagcg cggtgttcac gcgccccacc atgtgccgcg ggtagcggcg
     2701 cgagaacagc gcgtaggaca gcaccaccgc cgtcgagccg gcgaagaaca cgcaccacag
     2761 cgcgagcgca cccgcgcgca cgccgagcgc gagcatggcc aaagccacgg aagccgccgc
     2821 cacgccgccc gcgagcagcg gcagcgcgct gcggccgctg cgcacgcgcg catcggccgc
     2881 gcggccgaag ccgacgtagc cgagcatcat ggcgaggctg aacaggagca aagcgcgggc
     2941 gacctcggca gccgtatagc cggcgatgtc gcgcagccag gtcgccaccc agagcgttga
     3001 tagagacaca gcggccacct ggctcgcgcc gatgcacagc gccacgcgcc agaacgccgg
     3061 atcgcgcgcg atggcgccga agccgcgcag ttgcgcgccg acgctctcgc cgcgctgggt
     3121 cgcgctgcgc tccggaacga cgaggtagag gagcaggctg acggcgaggt tcagcgccac
     3181 gatgccggcg aacacctcgc gccagtggaa gacgcgcaac gccatctcca gcggcaccgt
     3241 ggccaccacc gcgccgagca tgccgctggc gaaggcgatg ccgttcatgc tggcgaggcg
     3301 ctcggacggg taccaaagtg cgaaggccga gaacgccgcc atcaggcagc ccgagacgcc
     3361 gaggccgatc aggccgcgcg ccgcggtcag ctccgccgcg ctgctgccgg acgcgaacca
     3421 gatgccgccg accgcggcca ccagcagcat ggtggcgttg acgcggcgcg gcccgtagcg
     3481 gtcgagcagc accccgagcg gcagctggaa taccgcaaag gcaaagaaga agatcgccgt
     3541 gagcaggccg agccccgcgg ccgtgagccc cagctcgcgc gcgatgaccg gcccgatgat
     3601 ggcgttcacc gagcggtaca ggtaggagat gaagtacccc gccaggaacg gcagggtgac
     3661 gcgcacgagc gggttcaggc ccgcgcctcg gtcgccggac cctcgatcac cagcatcagg
     3721 tcgtccgccc gcacggtcgt gtcgcccaga tcccagaaca ggatgcgctt cttgtcgcgc
     3781 tcgatgccga tcaccaggca cggcagcgcg cgcgcctcgc gcccgacctc gtcggggttc
     3841 ggcgcgcgct cggtgagccg cacgcggccg cgctcggtca gcagctcgtt gatcaggccg
     3901 acggtgtacg cgctttccac cgcgtcggcg aggaggtagc cgccgacgcg cgccggcgac
     3961 acgatcacgt ccgcgccgcc ctggcgcagg agcttcgcgt tctcgttctc gcgcacgctg
     4021 gcgatgatgc ggaccttgtc ggacaggtgg cgcgcggtga gcgtggcgag caccgcggtg
     4081 tcgtcgcgcc cgatgcacag cagcagcgcg cgcgcctgct gcaccaccgc gcggcgcatg
     4141 atctcctcgc tggtgcagct gcccaccagc gcgacgaagc cggcctcggc ggcggcgcgc
     4201 gccgcgccct cgctctggtc gatcaccacc acctgctcgg gcttctcgcc cttggcgacg
     4261 agctcgaccg cggcgatgcg cccgctgtgg ccgaagccgc agacgatgac gtggtcggac
     4321 agcgaatcct cgagctttct catgcggcgc tcctccatca tgcgctggaa caccatctga
     4381 taggcggtgc ccacgaatat gaacaggacg aaaaaccgga tcggcgtgac gaagaaggag
     4441 tcgatgaggc gcgcgcggtc ggtgaccggc acgatgtcgc cgtagccgac cgtggtcacc
     4501 gtcaccatcg tgaagtagac gacgtcgagg aacgagacca cgccgtcgga ggcgtcgcgc
     4561 aggccgtcgc gatccagcca gaacaccgcc agcaccgcgg cgaacagcac cagcaccagc
     4621 accacgcgta gcgccagggt gcgcgccgcg gtcgactctt cctggcgcac caggagccgc
     4681 acgcgccgtc ccgagccgcg ccgcgggaac agctgtacgc gcagcagccg tgcgagcagc
     4741 cgggactcct tgcgatcttc ggccatctcg gcgctgcggc cggcgggtac gctcagcgcg
     4801 gcggcggttc gatgccgccg acgtagaagc ggttccaggt cggcgccatg atgatctcgt
     4861 tcacgcaggt gcgtgccggc atcgtcgcga cgaacaggat cgctttgccg aagtcctcgg
     4921 cctgcgccat gcgcgcgcgc tcctcggcgc tcggcggcac ggggcgcttc tccatgatcg
     4981 gcgtggccac ttcgcccggc aggatcgagg tggcgcggat gccgttggtg cactcctcca
     5041 tgttgatcga ctcgctcacc gcgatcacgg cacgcttggt ggcgttgtag gcggggccgg
     5101 tcagcacgct tgcgtagcgc ccggcccagg aggagatgtt gatgatgacg ccgtctttgc
     5161 gctggcgcat gccgggcagc acggcgtagc agcagtagaa caggccgttc aggttgatct
     5221 tcaccacgtc gtcccagccc tcgagcgaca tggtcttcag gttacgcttg ccgccgccga
     5281 tgttggtgcc ggcgctggtg acgaggatgt cgatgcgccc gtggcgcttc tcgatctcgg
     5341 ccgcggcctt ctccaccgcg gcgcgctcgc cgacgtcgag caggttggct tccgcgctcc
     5401 ccagcttctt cagctcggcg aggccgcttt cattggtgcg cgcggtgcgc ccggagatga
     5461 tcacgtgcgc gccggccgcg gcgagcgcct ttgcgcccgc caggccgatg cccgagccgc
     5521 cgcccgtgat ccaggcgacc tttcccttca gctcagtgct tggcatattg cttggctccg
     5581 aacaggttct ccttctgctc cggcgtcatc tcggcgcggc ggcggtcggc gagcaccttg
     5641 agcgcgcgct ccaccgccgg acgcgcggcg atgccgtcgc gccagcggcg cacgttcggg
     5701 tactcctcga tgttcacgcc ctggcgctcg ggaaagcgca tccagggcat ggtggccatg
     5761 tcggcgatag tgtactcgtc gcacgcaagg tatttcgatt ccttgagccg ccggtccacc
     5821 acgccgtaca ggcggttggc ctcgttcttg tagcggttca tggcgtactc gattctctcg
     5881 ggcgcgtagc cgaggaagtg atgcgcctgc ccgagcatcg gcccgatgtg ccccatctgg
     5941 aacatcaccc actgcatcac ctgccagcgc ttgcggatgt cctcgggcag gaacctgccg
     6001 gtcttcgagg cgaggtaaaa gagcatcgcc cccgactcgg cgagcgccat cggcttgccg
     6061 tccgggccgt cgctgtccac catcgccggc atcttgttgt tgggcgagat cttgaggaac
     6121 tcgggcttga actggtcgcc cgcgccgatg tcgaccggga tcaccttgta gggcagtccc
     6181 gtctcctcca gcatgatgtg gatcttgtgc ccgttgggcg tgggccaggt atagaggtcg
     6241 atcatgtgag tttcaccagc tgcttgccga agttttcgcc cttgaggagc ccgatcaggc
     6301 ccttgggcgc gttctcgatg ccttgcacca ccgattcgcg gtacttgagc ttgccggccg
     6361 ccaccaggtc cagcaaggcc tggttcgctt ccgggtagcg cggctgccag tcgaagacga
     6421 tcatgccccg aaccgtgagg cggttgacga gaaccgagcg gatcatcttc acgccgtacg
     6481 gttccttcgc gttgtagtcc gagatcaggc cgcagacggc gatgcgcccg cgcacgttca
     6541 tgagccgcag cacggtgtcg aggatctccc cgcccacgtt ctcgaagtcg gcgtcgatgc
     6601 ccttcgggca cgcggccttc agctgcgcgt agacgtcgcc cgccttgtag tcgacgcagg
     6661 cgtcgaaccc cagctccttc accacgtagt cgcacttggc cttgccgccc gcgatgccca
     6721 ccgcgcggca gccccaggcc ttggcgagct ggccgacgac gctgcccacc gcgccggagg
     6781 ccgcggacac caccaccgtc tcgccgggct tgggctggca gatgtccttc atgccgaagt
     6841 aggcggtgcg ccccggcatg ccgaggcagc cgaggtaata ggagagcggc gccttggcgt
     6901 cgtccacctt ggcgagccgc tcgcccttca ggcacgagta cagctgccag cccccgccct
     6961 gcccggtcgc caccttgtcg ccgggcttga acctcgggtc cttcgattcc acgacttcgc
     7021 ccgcggtctc gccggtcatc acctcgcccg gctcgacgcc cttcacgtag ctcttctgct
     7081 ggctcatgcg gccgcgcatg tacgggtcga gcgacagcca gtggttcttt accagcactt
     7141 cgccctcgcc gggcttcggc acgggcgcgg tttccagccg gaaattggct tcggtgaccc
     7201 agccttccgg atagctggcg aggacgatgc gcttgttcat cgggggccct tcgggaaagg
     7261 agcgatgtta acgcaccacc cgcagcggca gcgcagccat gccgcggaac accatcgaat
     7321 tgagccactc gaggcgctcc tcgggctgcg cgagctcgat gcgcttccag tggcgcagca
     7381 ccgccggcag cgccacctgc ccttccatgc gggcaagcgg aaagccgagg cagatgtgca
     7441 ggccgaagcc gaaggtgagg tgcggcactt ccttgcggcg caggtcgagc cgctccggat
     7501 cgtcataggc ctcgggatcg cggttcgccg cattcagcat caggaacacg cgatcgcccg
     7561 gcgccaggcg cttgccgcgc atctcctgcg cgaccttcac cacgcgcact tgcgccccgc
     7621 tcggcccgtc gtagcgcagc agctcctcga cggcgcgcgg cgccagcgcc ggatcggcgc
     7681 gcagcgccgc agcctcgccc ggaaaccgga gcagcgccag catcgcgttg gcgatgtggt
     7741 tggtggtggt ctcgtgcccg gcgaagagca gcaggatgca ggtggcgacg agctcgtcct
     7801 cgctcagccg gtcgccatcg tcctccaggt gcacgagctg cgaaagcagg tccgcgcgcg
     7861 gcgcggcgcg gcgctcggcg atcagtccgc gaaagaacgc cgccatttcg cgcgtcgcgg
     7921 cctcggcgat ggcgtacttc tccggcgaca tgcgcgagct gccgatgaac agcgccatgt
     7981 cgtccgacag acgcttgatg accggcaggt ggtcgcgcgg cacgccgagc atcgccatga
     8041 tcaccagtcc cggcagcggt ccggcgaaat cggcgatgaa gtcgaacttc tcgcgatcgc
     8101 cgaggcgccc gagcagccac gcggcgagcg cctcgacctg ggggcgcatg gcgttcatgg
     8161 actgcaggtg gaacaccttg ctggtcagcc ggcgcaggcg cgtgtgctcg ggcgggtcgc
     8221 ggaacaccat ccagtgcgtg aggtagcgca tgatgtcgcc gatgcgctcg gcctccgggc
     8281 cgggcagcgt ggcaaagaac ggccgcaggc ggtcggagga catctcgcgg tcgaggagca
     8341 ccgcgcggca gtcgtcgtag cgcgtcagca cccaggcctt caggcgcggg ctccagtgca
     8401 cggggtcttc cgcgcgcaag gcgcgataca gcgggaacgg atcgcgcagg aactccggcg
     8461 cgtccgcgcg gaactctact gccaccgggg cttgcgcttc tcgaggaacg agcggacgcc
     8521 ctcgcgcgcc tcggcgctcg cgcgcgcctt ggtcatcttc gccgccttct ccatcttggc
     8581 gaggtcgccg cccgagcaga aggcggggcc gcgcggctgg atgtcgagga tcacgacagg
     8641 gccagccggc gccgcgccgc cgtgtactcg gcggcgagct tcgccaccac ctcgg
//
LOCUS       DBNHKE010000039         2846 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_269918, whole genome shotgun sequence.
ACCESSION   DBNHKE010000039 DBNHKE010000000
VERSION     DBNHKE010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_269918"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..989
                     /locus_tag="AC16O9_02250"
     CDS             81..989
                     /locus_tag="AC16O9_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6515470.1"
                     /translation="MIPRPVPTVRGMLWMAASGLLFIVLNTIMKRLSYDLDPWLVGFL
                     RYALGALVMVGTALPGGLAALWPKAPGLQLVRGMFHTGGMMLWFAALPFVTLAELTAI
                     GFSGPIFICIGAVLFLSERMTGARWTAVLVGFAGVLLVVKPGQAEGFAGISPGMLLML
                     ASAPVFAGSYLVAKALTRYDRSDVVVLWQHAWVSILLAPFALAFWSPPSAVQWALLVA
                     CGFLGAGGHYCMVRAFRVADISATQSVKFLELVWAAILGWIVFGTAPAGATVAGGVVI
                     LVSTLLLARHESRVTAPVPESAREGR"
     gene            986..2533
                     /locus_tag="AC16O9_02255"
     CDS             986..2533
                     /locus_tag="AC16O9_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6515471.1"
                     /translation="MSWQEEMDELARRKRLAAQMGGAEKVARHKGLGKLTVRERIDGL
                     VDAGSFHEIGSIAGKASYDAEGGLAGFIPANLVMGRARIEGRPVAVAGDDFTVRGGAA
                     DAAIRGKLVTVERMAQDLRIPLVRLVDGTGGGGSVKSIEIEGRTLLPSMPRWQLCVDN
                     LARVPGVALALGSVAGLGAARVAASHYSVIVKDTAQMFVAGPPVVARVGEEVGKNELG
                     GSEIHTRNGAIDDEAASETEAFALARRFLSYLPGSVHEFAPRTEPKDAPKRREEWLLG
                     AIPRDERKVYKMRPIVQALVDKDSFFEVGRRWGKSVITGLARLDGWPVAVLASDPYHY
                     GGAWTADACDKVIRMVDLAQTFQLPVVHLVDVPGFLIGRVAEEAGTIRHGARAMQAIY
                     QCTVPWCAILVRKVFGVAGAAHMNPARYGMRYAWPSGDWGSLPVAGGLEAAYKAELEA
                     APDPKAKLAEIEARLGKLKSPFRSAEAYIVEEIIDPRDTRTILTDFANLAAPLRSPGP
                     PSFGYRP"
     gene            2530..>2846
                     /locus_tag="AC16O9_02260"
     CDS             2530..>2846
                     /locus_tag="AC16O9_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6515472.1"
                     /translation="MTLRAALAPRSIAIVGASDNPHKVGGRPILYMKRYGFRGAIYPI
                     NPGRKEVQGLKAFPSIGDLPQAPDLAVIAVAGDEAVRAVEECAAKGVQVAVVMTSGFG
                     ELG"
BASE COUNT          383 a          942 c         1080 g          441 t
ORIGIN      
        1 cctcgcagcg cacgtgaaac gtcggcatgc gcgggcgatt atggcaggac aggcgtgcaa
       61 caatcgagcc cgaactcgaa atgatcccca gacccgtccc gacggtgcgc ggcatgctgt
      121 ggatggcggc ctcggggctg ctgttcatcg tcctcaacac gatcatgaag cgcctgtcct
      181 acgacctcga cccctggctc gtgggcttcc ttcgctatgc tctcggcgcg ctcgtcatgg
      241 tcgggacggc gctgcccggc gggctcgcgg cgctgtggcc gaaggcgccc gggctgcagc
      301 tggtgcgggg gatgttccac acgggcggga tgatgctctg gttcgccgcg ttgccgttcg
      361 tcacgctcgc cgagctgacc gcgatcggct tttccggccc gatcttcatc tgcatcgggg
      421 cggtactgtt cctgagcgag cgcatgacgg gggcgcgctg gacggcagtg ctggtcggct
      481 tcgccggcgt gctgctcgtg gtcaagccgg gccaggccga gggcttcgcc ggcatctcgc
      541 cgggcatgct cctgatgctc gcctcggcgc ccgtgttcgc cgggtcctat ctcgtcgcca
      601 aggcgctcac gcgctacgac cgctcggacg tcgtcgtgct gtggcagcac gcgtgggtgt
      661 cgatcctgct cgcgccgttc gcgctcgcct tctggagccc gccgagcgcc gtgcagtggg
      721 cgttgctcgt cgcctgcggc tttctcggcg cgggcggcca ctactgcatg gtgcgtgcgt
      781 tccgcgtcgc cgacatctcc gcgacgcagt cggtgaagtt cctggagctc gtgtgggcgg
      841 cgatcctcgg ctggatcgtc ttcggcacgg cgccggcggg cgcgacggtc gcgggcggcg
      901 tggtgatcct ggtcagcacg ctgctcctcg cgcgccacga atcccgcgtg acagcgcccg
      961 tgccagaatc cgcccgggag gggcgatgag ctggcaggag gagatggacg agctcgcgcg
     1021 gcgcaagcgc ctggccgcgc agatgggcgg cgccgagaag gtggcgcgcc acaaggggct
     1081 cggcaagctc acggtgcgcg agcgcatcga cggcctggtc gacgcgggca gcttccacga
     1141 gatcggcagc atcgccggca aggcgagcta tgacgccgag ggcgggcttg cgggcttcat
     1201 cccggcgaac ctggtgatgg ggcgcgcgag aatcgagggc cgcccggtcg cggtggcggg
     1261 agacgatttc accgtgcgcg gcggcgccgc cgacgcggcg atccgcggca agctggtcac
     1321 cgtcgagcgc atggcgcagg acctgcgcat cccgctcgtg cgcctggtcg acggcacggg
     1381 cggcggcggc tcggtgaagt cgatcgagat cgagggccgc acgctcctgc cgagcatgcc
     1441 gaggtggcag ttgtgcgtgg acaacctggc gcgggtgccg ggggtggcgc tcgcgctcgg
     1501 ctcggtcgcg ggcctgggcg cggcgcgcgt ggcggcgagc cactactcgg tgatcgtgaa
     1561 ggacacggcg cagatgttcg tcgcggggcc gccggtggtg gcgcgcgtgg gcgaggaggt
     1621 gggcaagaac gagctcggcg gcagcgagat tcacacgcga aacggagcga tcgacgacga
     1681 ggccgcgagc gagacggagg cctttgcgct cgcgaggcgc ttcctgtcgt acctccccgg
     1741 ttcggtccac gagtttgcgc cgcgcaccga gccgaaggac gcgccgaagc ggcgcgagga
     1801 gtggctgctc ggggcgatcc cgcgcgacga gcgcaaggtc tacaagatgc gcccgatcgt
     1861 gcaagcgctc gtcgacaagg actccttctt cgaggtcggg cggcgctggg gcaaatcggt
     1921 gatcacggga ctggcgcggc tggacggctg gccggtggcg gtgctggcga gcgatccgta
     1981 ccactacggc ggcgcctgga ccgcggacgc ctgcgacaag gtgatccgca tggtggacct
     2041 cgcgcagacc ttccagctgc cggtggtgca cctggtcgac gtgccgggct ttctcatcgg
     2101 ccgcgtggcg gaggaggcgg gcaccattcg ccacggcgct cgcgccatgc aggcgatcta
     2161 ccagtgcacg gtgccctggt gcgcgatcct ggtgcgcaag gtcttcggcg tcgcgggcgc
     2221 agcgcacatg aatccggcgc gctacggcat gcgctacgcc tggccctcgg gcgactgggg
     2281 ctcgctgccg gtcgcgggcg ggctcgaggc cgcgtacaag gccgagctcg aggccgcgcc
     2341 cgatccgaag gcgaagctcg ccgagatcga ggcgcgcctc gggaagctca agtcgccttt
     2401 tcgcagcgcc gaagcctaca tcgtcgagga gatcatcgac ccgcgcgaca cccgaacgat
     2461 cctgacggac ttcgccaacc tggcagcacc gctgaggtcg ccggggccgc cgagcttcgg
     2521 ctaccggcca tgacgctgcg ggcggcgctt gcgccgcgct cgatcgccat cgtcggcgcc
     2581 tccgacaacc cgcacaaggt cggcgggcgg ccgatcctgt acatgaagcg ctacggcttt
     2641 cgcggcgcca tctatccgat caatccgggg cgcaaggagg tgcaaggctt gaaggccttt
     2701 ccctcgatcg gcgacctgcc ccaggcgccc gatctcgccg tcatcgcggt ggcgggcgac
     2761 gaggcggtgc gcgcggtcga ggagtgcgcg gcgaagggcg tgcaggtcgc cgtggtgatg
     2821 acctcgggct tcggcgagct gggcga
//
LOCUS       DBNHKE010000040        12906 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_163819, whole genome shotgun sequence.
ACCESSION   DBNHKE010000040 DBNHKE010000000
VERSION     DBNHKE010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_163819"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC16O9_02265"
     CDS             complement(<1..465)
                     /locus_tag="AC16O9_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6515473.1"
                     /translation="MPEIDARAARRRFGRAAATYADAARLEGEVGARMLERLEVVRVA
                     PARILDAGCGPAPQARALARRYPGAALVALDFSLPMLALARRALGWRARLRGARMLAV
                     CGALERLPLAAGSIGLAWSNMALHWAADTHAALAELGRVLVPGGLLMFSTLGP"
     gene            complement(458..1804)
                     /locus_tag="AC16O9_02270"
     CDS             complement(458..1804)
                     /locus_tag="AC16O9_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6515474.1"
                     /translation="MWTVERLSRELAEGRTTSRALVEEALARIADPAGEGARAFVKVY
                     AEQARLEAGHADMLRRAGIVRSPVDGLPVSVKDLYDVGGDVTRAGSKLLAGAAPAKAD
                     APAVARLRAAGAVIVGRTNMVEFAFGTSGLNPHYGTPKSPWDRKTGRVPGGSSSGAGV
                     AVADGMCVMGLGSDTRGSVRVPAAFCGIAGWKPTQRRVPRDGAFPLSYTLDTVGPLAN
                     TLACCAAYDAVLADTPLEPLPELPVKGLRLLLPKSALLGDLDADVSRAFTAALKKLSA
                     AGALITERAVPEFDRQAEYFRLGGYAGAEAYALHRRWLERRAEYDPRVAKRIDLAAQL
                     SAADYVDLGLMRAAYIRAVEALAAPFDAILMPSVPCVPPPIAEVDASDEAYFAWNGRF
                     LKNVGVVNFLDGCGAALPCHEPGGGPVGLTVFGPAMSDRRVLGAALAIEKLLRPLA"
     gene            1833..2534
                     /locus_tag="AC16O9_02275"
     CDS             1833..2534
                     /locus_tag="AC16O9_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR6515475.1"
                     /translation="MLGPLARRFANLAFGGSCYLCRGAADAVLCAACDADLPRLAGPR
                     CPRCALASPAGEVCGRCLARPPAFDATVAALAYAFPADVLVQALKFRGELALAPLLGR
                     LLAARLERAERVDVVLPVPLSAARLRARGFNQALEIARHLGPDGGARLEPRLAERSRD
                     TAAQMDLPHAERAKNVRGAFRCARALEGAAVALVDDVMTTGATLDELAATLKRAGAAR
                     VVNWVLARTPAPADA"
     gene            2527..2991
                     /locus_tag="AC16O9_02280"
     CDS             2527..2991
                     /locus_tag="AC16O9_02280"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O-ribose)-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR6515476.1"
                     /translation="MLDVVLYRPEIPPNAGNAIRLAANTGARLHLIEPLGFTLEDRQL
                     KRAGLDYHDMASVHVHRDWNACRAALAGRRLFAVSTRGAVSYAEVRYAVDDAFVFGPE
                     TSGLPQALLEQFAPERRLRLPMRAGNRSLNLSNAVAVVVFEAWRQLGFRGAG"
     gene            complement(2918..3964)
                     /locus_tag="AC16O9_02285"
     CDS             complement(2918..3964)
                     /locus_tag="AC16O9_02285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR6515477.1"
                     /translation="MTRIGVLGAGAWGTALAAALSARHEVTLWARDPAQAAAIGSARL
                     NARYLPEVPLPAALTVTDRWPARAHLLVVATPVAGLREVLARAAPVPLIWLCKGFEAG
                     TGLMPHQVVAEVLGARAACGALSGPSFALEVARGLPCALTLASKNAAFASAAAKTLHG
                     GRLRVYHSTDLVGVEVGGAVKNVMAIAVGIADGLGLGQNARAALITRGLAEMQRLGVA
                     LGGSPETFMGLAGAGDLILTATGDLSRNRRVGLALAQGEALADILARLGHVAEGVHSA
                     RETLRRAAQAGVEMPITQAVQAVLQGRLTPAQAVERLLARDPKAEARPASPRRGSRAG
                     ARPRKPPPRRRSKG"
     gene            complement(3961..4398)
                     /locus_tag="AC16O9_02290"
     CDS             complement(3961..4398)
                     /locus_tag="AC16O9_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR6515478.1"
                     /translation="MMRRAAAALVVALAAGAAHAQFLSVGENAAILYDAPSRAAKPLF
                     VVSRQYPLEVIVNLEAWVKVRDHAGALTWVERKSLVEQRTVVIIAPSAEARVRPEEGA
                     PVAFVAAQNVVLELLGMVPGGWLRVRHPDGANGYLHSSAVWGA"
     gene            complement(4403..4867)
                     /gene="secB"
                     /locus_tag="AC16O9_02295"
     CDS             complement(4403..4867)
                     /gene="secB"
                     /locus_tag="AC16O9_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR6515479.1"
                     /translation="MAEEQQPSFQIEKIYVKDLSLEIPGAPQVFLEAQTPQLEIQVRN
                     ESSRFAEGLFEVVITVTVTAKAGEKTLFLAEAAQAGIFSLRNVPEADLEPLLAVACPT
                     ILYPYAREAVSDAVTRGGFPPVLLAPVSFEAIYAQRQQQKAGGGAPQAEAAG"
     gene            complement(4872..5123)
                     /gene="grxC"
                     /locus_tag="AC16O9_02300"
     CDS             complement(4872..5123)
                     /gene="grxC"
                     /locus_tag="AC16O9_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HIR6515480.1"
                     /translation="MYLTAACPYCQAAQRLLAQKGVSDVQAVRVDLEPQRRDEMRALS
                     GRHTVPQIWIGGRHVGGFDELVALERAGELDPLLETQRT"
     gene            complement(5140..5550)
                     /locus_tag="AC16O9_02305"
     CDS             complement(5140..5550)
                     /locus_tag="AC16O9_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6515481.1"
                     /translation="MLEFLQKNILLVAAAVGSGAMLLWPLVRGGVGGASVNTLQATQL
                     MNRQNALVLDVREPAAYAAGHILGAKNLPLAEIDKRAEDFAKHKTKTVIVHCDTGSTA
                     TRAVALLKARGFENVFNLSGGFRAWLQGGLPVEK"
     gene            5575..6315
                     /gene="gpmA"
                     /locus_tag="AC16O9_02310"
     CDS             5575..6315
                     /gene="gpmA"
                     /locus_tag="AC16O9_02310"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR6515482.1"
                     /translation="MHRIVFLRHGESTWNRENRFTGWTDVDLSPAGVEEARAAGRLLA
                     AEGYAFDIAHTSVLKRAIRTLWLVLEEMDCCWLPVLKSWRLNERHYGDLQGLNKAEMA
                     AKFGEAQVLAWRRSYDTPPPALKAGDPRYEGRDPRYAGAEVPLTECLKDTVARVLPYW
                     NGTIAPAVRAGKRVLVAAHGNSLRALIKHLDGVSDAEIVGLNVPTGIPLVYELDAELK
                     PLKHYYLGDAAEIARRVAAVSAQGKAKA"
     gene            6333..7472
                     /locus_tag="AC16O9_02315"
     CDS             6333..7472
                     /locus_tag="AC16O9_02315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6515483.1"
                     /translation="MLAIAAASAAHAGREEDLAALRARIEALKAGLGEKEADRREARD
                     ALRDSERAISEANRTLRKLEAEGRAAQAELARLGAQSRSLGERLARQQAALGRLLAVR
                     HAAGAPDALKIALSGENPSDVARRLYYLAELSRATARLGEALRANLAEAEAVRRAAAA
                     RRERLAAIEAESRRDRARILAERRERRTILDRVSGDIRKARRQIGTLQADERRLARLV
                     EEIVHVLKARPGAGFARVESVPQLGSLAGPFSGLRGKLRLPVKGELVALFGAPRQEGP
                     VGKKGVFIRAKEGQPVLAVAAGQVVHADWMRGYGNLLIVDHGEAYLSIYANNESLLKQ
                     TGDPVAAGEIVATVGASGGNEQSGLYFELRHLGQAFDPLRWVVLK"
     gene            7488..8897
                     /locus_tag="AC16O9_02320"
     CDS             7488..8897
                     /locus_tag="AC16O9_02320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6515484.1"
                     /translation="MSKIRTFGLIALGAVAGVLLSLNFQAMADRVARGPLPVDELRAF
                     TEVFGAIKQNYVEPVEDKKLITEAINGMLSGLDPHSAYLDADAFKELQVGTQGQFGGL
                     GIEVGMEDGFVKVVSPIEDTPAFRAGIKPGDLIIRLDDTPVKGMTLADAVKRMRGKPN
                     TQITLTISRKGEARPIVVTLTRAVIRVQSVKSKIIEPGYGWVRVSQFQESTTEHLVRH
                     ISNLYKQEGELKGLVLDLRNDPGGLLYGAVAISSAFLPEKVLVVSTDGRTEDAKKKFF
                     ATPDDYLRGMRDDVLRKLPAGAKTVPMVVLVNGGSASASEIVAGALQDHKRATVIGTQ
                     TFGKGSVQTIMPLGNATAIKLTTARYYTPSGRSIQAKGITPDVIVEEPGASAAARLRE
                     ADLERHLENDRDAAAAAEKKGTPRARTVPEAPAGELPKPTELGSKDDFQLAQALNFLQ
                     GKPVLGQPSKAVAENKSGS"
     gene            8894..9637
                     /locus_tag="AC16O9_02325"
     CDS             8894..9637
                     /locus_tag="AC16O9_02325"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR6515485.1"
                     /translation="MNDQQLLRYSRHILLEEFGIEGQERLRAAAALIVGAGGLGCPAA
                     LYLAASGIGRLTIADADSVDLTNLQRQILYRTDSVGRVKVEEARKALAAVNPEVEIVP
                     LALRVADADVDRLVAAADVVLDCSDNFATRHALNRACVKYSRPLVSGAAIRFDGQLMV
                     FDLRRKDAPCYACLFPEDGEVEDVQCSTMGVLAPLTGAIGAMQALEAVKLLAEAGDTP
                     LGQLTLFDAKASELRSVKVAKDPGCAVCA"
     gene            9651..10577
                     /locus_tag="AC16O9_02330"
     CDS             9651..10577
                     /locus_tag="AC16O9_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515486.1"
                     /translation="MPLYYTGAVNAPEQAEAQFDRAAQDVGNLVLLEHFNVTIADQRL
                     ATLFYVVGLGGTRDPYLFTGFTNMWVNFGRTQAHLPSRGAQPRAQVLRGSAGFVVPDL
                     EALKKRLDFAGAEMKRVVPEAATKFSWREQGGCIEATCPWGNRIRCHAPGPEFGRTEL
                     GLVYVDFDVPPGTAAGIARFYAEVMRAPASAEGGRATVRIGRGQRLLFTESSEPVAAY
                     DGHHVQVYIADFSSPYAWLKARGLVTMESDAHEWRFQRIVDPQSLEPLFEIEHEVRSM
                     THPLYGRPLVNRNHAVTNVNYRPGQDAFRGTY"
     gene            complement(10623..12317)
                     /locus_tag="AC16O9_02335"
     CDS             complement(10623..12317)
                     /locus_tag="AC16O9_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6515487.1"
                     /translation="MVARTTAKIPAAPAGDAREALLAGLPVAERRLTLAGVATAVLEG
                     GTGAPLVLLHGPGEHATKWLRVLPDLAKRHRVIAPDLPGHGASNIEGGALDVEGVLAW
                     LDALIERTCSEPPVLVGQIVGGAIAARYAARHGARLKALVLADALGLAPFQPAPEFGH
                     ALMEFSAQPSEENHDRLWARCAYDLDAMRERMGERWHALRAYNLDRARTPGVQAATGG
                     LMQQFGMPAIPEAELARIGVPTTLIWGRHDLATPLGVAERASRRFGWPLHVIERAADD
                     PPMEQPEAFVRALQAALREDTRAAWDAIAPGYDRTNTPTQMWLAGEGLRRAGLHAGMS
                     FLDVAAGSGALSLPAARLGAQVMAVDQSPAMLELLQARARKEKLEIETRVMDGHALAL
                     AENQFDLAGSQFGVMLFPDMPQGIREMVRAVKPGGRVLVHAYGDPHRIDFLGFLVGAL
                     QSVRPGFAGPPMDPPPLPFQLADPARLRAELAGAGLKGVRVETVTETTAFRDGEALWD
                     WIVCSNPIVDAVLGSLNLTADEIDTARGALDRLLRERGAGGVAQLSNPVHIGIGSK"
     gene            complement(12454..>12906)
                     /locus_tag="AC16O9_02340"
     CDS             complement(12454..>12906)
                     /locus_tag="AC16O9_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6515488.1"
                     /translation="QARGALELAAGAPRQALAPLRHAAQAWRQLGDPYEAARVRALLG
                     AACRALGDEDGGALEIAAARGEFARLGAAPDLARLEAPARGRGPNLTARELQVLRLVA
                     TGKTNKGIAAALELSEKTVDRHLSNILTKLDVPSRAAAVAYAYEKKLI"
BASE COUNT         1850 a         4687 c         4662 g         1707 t
ORIGIN      
        1 gggcccgagc gtgctgaaca tcagcagtcc gccggggacg agcacgcgcc cgagctcggc
       61 gagcgcggcg tgcgtgtcgg ccgcccagtg cagcgccatg ttggaccagg cgagcccgat
      121 cgagcccgcc gcgagcggca ggcgctcgag cgccccgcac actgcaagca tgcgcgcccc
      181 acgcaggcgc gcgcgccagc cgagcgcgcg gcgcgccagc gcgagcatcg gcagcgaaaa
      241 atcgagcgcc acgagcgccg ccccggggta gcggcgcgcc agcgccctgg cctgcggcgc
      301 cggcccgcag cccgcatcca ggatgcgcgc aggcgcgacg cgcaccacct cgagccgctc
      361 gagcatgcgc gcgccgacct cgccctccag gcgcgcggcg tccgcatagg tggccgcggc
      421 gcggccgaag cggcggcgcg cggcgcgcgc gtcgatctca ggcaagcggc cgcagcagct
      481 tctcgatggc gagcgcggcg ccgagaacgc gccggtcgct catcgcaggg ccgaacacgg
      541 tcaggcctac gggcccgccc ccgggctcgt ggcacggcag cgccgcgccg caaccgtcga
      601 ggaaattgac cacgccgacg ttcttcagga agcgcccgtt ccaggcgaag taggcctcgt
      661 cgctcgcgtc cacctcggcg atcggcggcg gcacgcacgg cacgctcggc atcaggatgg
      721 cgtcgaacgg cgcggcgagc gcttccaccg cgcggatgta ggcggcgcgc atgagcccga
      781 ggtcgacgta gtccgcggcg ctcaactgcg ccgcgaggtc gatgcgcttc gcgacgcgcg
      841 gatcgtactc ggcgcgacgc tccagccagc ggcggtgcag ggcgtaggct tccgcgccgg
      901 catagccgcc gaggcggaag tactcggcct ggcggtcgaa ctcgggcacc gcgcgctcgg
      961 tgatcagcgc gccggccgcg gagagcttct tcagcgccgc cgtgaaggcg cgcgagacgt
     1021 ccgcgtcgag gtccccgagc agcgcggatt tcggcagcag caggcgcaga ccctttaccg
     1081 gcagctcggg cagcggctcg agcggcgtat cggcgagcac cgcgtcgtag gccgcgcagc
     1141 aggcgagcgt gttggcgagc gggccgacgg tgtcgagcgt ataggagagc gggaacgcgc
     1201 cgtcgcgcgg cacacgccgc tgcgtcggct tccagccggc gatgccgcag aaggccgccg
     1261 gcacgcgcac cgagccgcgc gtatcgctgc ccaggcccat cacgcacatg ccgtcggcca
     1321 ccgcaacccc cgcgccggag gaggaaccgc ccggaacgcg gccggtcttg cggtcccagg
     1381 ggctcttcgg cgtgccgtag tgcgggttga ggccgctggt gccgaaagcg aactcgacca
     1441 tgttggtgcg cccgacgatc accgcgcccg ccgcgcgcag ccgcgccacc gcgggcgcgt
     1501 cggccttggc cggcgccgcg ccggccagga gcttcgagcc cgcacgcgtc acgtcgccgc
     1561 cgacgtcgta caagtccttc accgacaccg gcaggccatc cacaggcgag cgtacgatgc
     1621 cggcgcgacg gagcatgtcc gcgtgcccgg cctcgaggcg cgcctgctcg gcgtacacct
     1681 tgacgaaggc gcgcgcgccc tcgcctgcgg gatcggcgat gcgcgcgagc gcttcttcga
     1741 ccagcgcgcg gctcgtcgtc cggccctcgg cgagctcgcg cgacaggcgc tcgaccgtcc
     1801 acatgaagtg agtctagcgc acaatattcg cgatgctcgg tcccctggcg cggcgctttg
     1861 cgaacctggc gttcggcggc agctgctacc tgtgccgcgg cgccgccgat gccgtgctgt
     1921 gcgccgcgtg cgacgcggac ctgccacggc tcgcggggcc gcgctgcccg cgctgcgcgc
     1981 tcgcttcgcc cgcgggcgaa gtctgcgggc gctgcctcgc gcgcccgccg gcgttcgacg
     2041 ccaccgtcgc cgcgctcgcc tacgcgtttc ccgccgacgt gctggtgcaa gcgctgaagt
     2101 tccgcggcga gctcgcgctc gcgccgctgc tcggccggct ccttgccgcc cggctcgagc
     2161 gcgccgagcg cgtggatgtc gtgctgccgg tgcccctttc ggcagcgcgc ctgcgcgcgc
     2221 gcggctttaa ccaggcgctc gagatcgcgc gccacctcgg gcccgacggc ggcgcacgcc
     2281 tcgagccgcg cctggccgag cgcagccgcg acaccgccgc gcagatggac ctgccgcacg
     2341 ccgagcgcgc gaagaacgtg cgcggcgcgt ttcgctgcgc gcgcgccctg gaaggcgccg
     2401 cagtggcgct ggtcgacgac gtcatgacca ccggcgcgac gctcgacgag ctcgccgcga
     2461 cgctgaagcg cgccggcgcc gcgcgcgtcg tcaactgggt gctggcgcgc accccggcgc
     2521 ccgccgatgc ttgacgtcgt cctctaccgt ccggagatcc cgccgaacgc cggcaacgcg
     2581 atccggctgg cggccaacac cggcgcgcgc ctgcacctca tcgagccgct cggcttcacg
     2641 ctcgaagacc ggcagctcaa gcgcgccggg ctcgattacc acgacatggc gagcgtgcac
     2701 gtgcaccgcg actggaacgc ctgccgcgcg gcgctcgccg gccggcggct gttcgccgtg
     2761 tcgacgcgcg gcgcggtgag ctacgccgag gtgcgctacg ccgtggacga tgcgttcgtg
     2821 ttcggcccgg agacgagcgg actgccgcag gccctgctgg agcaattcgc gcccgagcgg
     2881 cgactgcgcc tgccgatgcg cgcgggcaac cgcagcctca acctttcgaa cgccgtcgcg
     2941 gtggtggttt tcgaggcctg gcgccagctc ggcttccgcg gcgcgggcta gcgggccgcg
     3001 cttcggcctt cgggtcgcgc gcaagcaatc gctcgaccgc ctgcgccggc gtcagccggc
     3061 cctgcagcac cgcttgcacc gcctgcgtga tcggcatctc gacgccggcc tgcgcggcgc
     3121 ggcgcagcgt ctcgcgcgcc gaatgcaccc cctcggcgac gtggccgagg cgcgcaagga
     3181 tgtccgcgag cgcctcgccc tgcgcgagcg ccaggccgac gcggcggttc ctcgacaggt
     3241 cgcccgtggc ggtgagaatc aggtcgcccg cgccggcgag gcccatgaag gtctcgggcg
     3301 aaccgcccag cgccacgccg aggcgctgca tctcggcgag cccgcgcgtg atgagcgcgg
     3361 cgcgcgcgtt ctgcccgagg ccgaggccgt cggcgatgcc cacggcgatc gccatcacgt
     3421 tcttcacggc gcccccgact tcgacgccga cgaggtcggt gctgtgatac acgcgcaggc
     3481 gcccgccgtg aagcgttttc gcggcggcac tcgcgaatgc ggcgttcttc gaggcgagcg
     3541 tcagcgcgca gggcaggccg cgcgccactt cgagcgcgaa cgaggggccg gagagcgcgc
     3601 cgcaggccgc gcgcgcgccg agcacctcgg ccaccacctg gtgcggcatc aggccggtgc
     3661 cggcttcgaa gcccttgcac agccagatga gcggcaccgg cgcggcgcgc gcaagcacct
     3721 cgcgcaggcc ggcgaccggc gtcgccacca ccagcaggtg cgcccgcgcc ggccagcggt
     3781 ccgtcaccgt cagcgcggcg ggcaggggaa cctcgggcag gtagcgcgcg ttgaggcgcg
     3841 cgctgccgat cgccgcagcc tgcgccggat cgcgcgccca gagcgtcacc tcgtgccttg
     3901 ccgagagcgc cgcggcgagc gccgtgcccc aggcgcccgc tccgagcacg ccgatgcggg
     3961 tcacgccccc cagacggcgc tcgagtgcag gtagccgttg gccccgtcgg gatggcgcac
     4021 gcgcagccac ccccccggca ccatgccgag aagctccagc accacgtttt gcgcggcgac
     4081 gaacgcaacc ggcgcgccct cctcggggcg cacgcgcgcc tcggccgagg gcgcgatgat
     4141 caccacggtg cgctgctcga ccagggactt gcgctccacc caggtgagcg cgccggcgtg
     4201 gtcgcgcacc ttcacccagg cttcgaggtt gacgatcacc tcgagcggat actggcgcga
     4261 cacgacgaac aggggcttgg ctgcgcgcga gggcgcgtcg tagaggatgg cggcgttctc
     4321 gcccacgctc aggaactgcg cgtgcgcggc accggcggcg agcgcgacga cgagggcggc
     4381 ggcagcgcgg cgcatcatgg cgctagcccg ccgcctcggc ctggggcgcg ccgccgccgg
     4441 ccttttgctg ctggcgctgc gcgtagatcg cctcgaacga caccggcgcg agcagcacgg
     4501 gcggaaagcc gccgcgcgtc actgcatccg acaccgcctc gcgcgcatac gggtagagga
     4561 tggtcgggca ggccaccgca agcagcggct cgaggtccgc ctcgggcacg ttgcgcaggg
     4621 aaaagatgcc ggcctgcgcg gcctcggcga ggaacaaggt cttctcgccc gccttggcgg
     4681 tcaccgtgac ggtgatcacc acctcgaaca ggccctcggc gaagcgcgac gattcgttcc
     4741 tcacctggat ctcgagctgc ggcgtctgtg cctcgaggaa tacctgcggc gcgccgggaa
     4801 tctcgagcga gaggtccttc acgtagatct tctcgatctg gaagctgggc tgctgctctt
     4861 cggccatggg gtcaggtcct ctgggtttcg agcagggggt cgagctcgcc cgcgcgctcc
     4921 agcgcgacca gctcgtcgaa gccgccgaca tggcggccgc cgatccagat ttgcggcacc
     4981 gtgtgccggc ccgagagcgc gcgcatctcg tcgcggcgct gcggctcgag gtcgacgcgc
     5041 acggcctgca cgtcgctcac gcccttttgc gccagcaggc gctgggccgc ctggcagtag
     5101 gggcaggcgg cggtcaggta catgagcacg cgcgccaaat cacttctcca ccggcagtcc
     5161 gccctgcagc caggcgcgaa agccgccgga gaggttgaac acgttctcga agccgcgcgc
     5221 cttgagcagc gccacggcgc gcgttgcggt gctgccggtg tcgcaatgca cgatcaccgt
     5281 cttcgtcttg tgcttggcga agtcttcggc acgcttgtcg atttcggcga gcggcaggtt
     5341 cttcgcgccg aggatgtggc ccgcggcgta ggccgccggc tcgcgcacgt cgagcaccag
     5401 ggcgttctgc cggttcatca gctgcgtcgc ctgcagcgta ttcaccgagg cgccgccgac
     5461 ccccccgcgc accagcggcc acagcagcat cgccccggag ccgaccgccg cggcgacgag
     5521 caggatgttc ttctgcagga attcaagcat gaacggaaca ttataattga ccgcatgcac
     5581 cgcatcgtgt tcctgcgcca cggcgaatcg acgtggaacc gcgagaaccg cttcaccggc
     5641 tggaccgacg tcgacctgtc gccggcgggc gtcgaggagg cgcgcgccgc cgggcggctg
     5701 ctcgctgccg aaggctacgc cttcgacatc gcgcacacct cggtcctgaa gcgcgccatc
     5761 cgcaccctgt ggctcgtgct ggaggagatg gactgctgct ggctgccggt gctgaagagc
     5821 tggcgcctga acgagcgcca ctacggcgac ctgcaaggcc tgaacaaggc cgagatggcg
     5881 gcgaagttcg gcgaagcgca ggtgctcgcc tggcggcgca gctacgacac gccgccgccc
     5941 gcgctcaagg ccggggatcc ccgctacgag ggccgcgacc cgcgctatgc cggcgccgag
     6001 gtgccgctca ccgaatgcct gaaggacacc gtggcgcgcg tgctgccgta ctggaacggc
     6061 acgatcgcgc cggcggtgcg cgcgggcaag cgcgtgctgg tggccgcgca cggcaactcg
     6121 ctgcgcgcgc tgatcaagca cctcgacggc gtgtcggatg ccgagatcgt cggcctgaac
     6181 gtgcccaccg gcatcccgct cgtgtacgag ctcgacgccg aactgaagcc cctcaagcac
     6241 tactacctgg gtgacgccgc ggagatcgcg cgccgcgttg ccgcggtctc ggcccaggga
     6301 aaggcgaagg cctgaggcgc gcgctcctcg tcgtgctggc gatcgccgcc gcgagcgcgg
     6361 cgcacgccgg ccgcgaggag gacctggcgg cgctgcgcgc gcgcatcgag gccctgaaag
     6421 cggggctggg cgagaaggag gccgaccggc gcgaagcgcg cgacgcgctg cgcgattccg
     6481 agcgcgccat atcggaggcc aatcgcacgc tgcgaaagct ggaggccgag ggacgcgccg
     6541 cgcaggccga gctcgcgcgc ctgggcgcgc agagccgctc gctcggcgag cgcctcgcgc
     6601 gccagcaagc cgcgctcggg cggctgctcg ccgtgcgcca cgccgccggc gcccccgacg
     6661 cgctgaagat cgccctctcg ggggagaacc cgagcgacgt cgcgcggcgc ctgtactacc
     6721 tcgcggagct ctcgcgcgcc accgcgcgcc tgggcgaggc gctgcgcgcg aacctcgccg
     6781 aagccgaggc cgtgcgccgc gcggccgcag cgcggcggga gcgcctggcg gcgatcgagg
     6841 ccgagagccg gcgcgaccgc gcgcgcatcc tcgccgagcg gcgcgagcgg cgcacgatcc
     6901 tcgaccgggt ttccggcgac atccgcaagg cgcgccgcca gatcggcacc ctgcaggcgg
     6961 acgagcggcg cctggcgcgc ctggtggagg aaatagtgca cgttctgaag gcgcgcccgg
     7021 gcgcgggctt cgcgcgcgtg gagtcggtgc cgcagctggg ctcgctcgcc gggccgttct
     7081 ccgggttgcg cgggaagctg cgcctgccgg tgaaagggga actcgtcgcc ctgtttggcg
     7141 caccaaggca ggagggccct gtcggcaaga agggcgtctt catccgcgcc aaggaaggcc
     7201 agccggtgct tgccgtggcc gcggggcagg tggtgcatgc cgactggatg cgcggctacg
     7261 gcaacctgct gatcgtggac cacggcgagg cgtatctgtc catctacgcc aacaacgagt
     7321 cgctgctcaa gcagacgggc gacccggtgg ccgcgggcga gatcgtcgcc acggtcgggg
     7381 cgagtggggg caacgagcaa tcgggtttat actttgaact caggcacctc ggtcaggctt
     7441 tcgacccgct gcgctgggtg gtgctgaagt aaggatcgaa cgacatcatg agcaagatca
     7501 ggacctttgg gctcatcgcg ctcggcgccg tcgccggcgt gctgctcagc ctcaatttcc
     7561 aggccatggc cgaccgcgtc gcgcgcggcc cgctgccggt ggacgagctg cgcgcgttca
     7621 ccgaggtgtt cggcgccatc aagcagaact acgtcgagcc ggtcgaggac aagaagctca
     7681 tcaccgaagc gatcaacggc atgctctcgg ggctcgaccc gcactcggcg tacctcgacg
     7741 ccgacgcctt caaggaactg caggtcggca cgcagggcca gttcggcggc ctcggcatcg
     7801 aggtcgggat ggaggacggc ttcgtcaagg tggtctcgcc gatcgaggac acgccggcgt
     7861 tccgtgccgg catcaagccc ggcgacctga tcatccggct cgacgacacg ccggtgaagg
     7921 gcatgacgct cgccgacgcg gtgaagcgca tgcgcggcaa gcccaacacg cagatcacgc
     7981 tcaccatctc gcgaaagggc gaggcgcggc cgatcgtcgt caccctgacg cgcgcggtga
     8041 tccgcgtgca aagcgtcaag tcgaagatca tcgagcccgg ctacggctgg gtgcgcgtgt
     8101 cgcagttcca ggagtcgacc accgagcacc tggtgcggca catttcgaat ctgtacaagc
     8161 aggagggcga gctgaagggc ctggtgctcg acctgaggaa cgacccgggc gggctgctct
     8221 acggcgcggt ggcgatctcc tcggcgttcc tgccggaaaa ggtactggtc gtgtccaccg
     8281 acggccgcac cgaggacgcg aagaagaagt tcttcgccac acccgacgac tacctgcgcg
     8341 gcatgcgcga cgacgtgctg cgcaagctgc ccgccggcgc caagaccgtg ccgatggtgg
     8401 tgctggtgaa cggcggctcg gcctcggcct ccgagatcgt cgccggcgcg ctgcaggacc
     8461 acaagcgcgc gaccgtgatc ggcacccaga ccttcggcaa gggctcggtg cagaccatca
     8521 tgccgcttgg caacgcgacc gcgatcaagc tcaccacggc gcgctactac acgccgagcg
     8581 gccgttccat ccaggcaaag ggcatcacgc ccgacgtcat cgtcgaggag ccgggcgcga
     8641 gcgcggcggc gcgcctgcgc gaggcggacc tggagcgcca cctcgagaac gaccgcgacg
     8701 ccgcggccgc ggcggaaaag aagggcacgc cgcgcgcgcg caccgtgccg gaggcgcccg
     8761 cgggcgagct gcccaagccg accgagctcg gctcgaagga cgatttccag ctggcgcagg
     8821 cgctgaactt cctgcaaggc aagccggtgc tgggccagcc ctcgaaggcc gtcgccgaga
     8881 acaagtccgg cagctgaacg accagcagct ccttcgctac tcccgccaca tcctcctcga
     8941 agagttcggc atcgagggcc aggaaaggct gcgggcggcg gcggcgctca tcgtcggcgc
     9001 gggcgggctg ggctgcccgg cggcgctgta cctcgcggcg agcggcatcg ggcgattgac
     9061 gattgcggat gccgactcgg tcgacctcac caacctgcag cgccagatcc tctaccgcac
     9121 cgattccgtc gggcgggtaa aggtcgagga agcgcgcaag gcgctcgcgg cggtgaaccc
     9181 ggaagtcgag atcgtgccgc tcgcgctgcg cgtcgccgac gccgacgtcg accgcctggt
     9241 ggccgcggcg gacgtggtgc tcgactgctc ggacaacttc gccacgcgcc atgcgttgaa
     9301 ccgcgcgtgc gtcaagtatt ccaggccttt ggtctccggc gcggcgatcc gcttcgacgg
     9361 gcagctgatg gtgttcgacc tgagaaggaa ggacgcgccc tgctacgcgt gcctgtttcc
     9421 cgaggacggc gaggtggagg acgtgcagtg ctcgacgatg ggcgtgctcg cgcccctgac
     9481 cggcgccatc ggcgcgatgc aggcgctcga agcggtgaag ctgctggccg aagcgggcga
     9541 cacgccgctc gggcagttga cgctgttcga cgccaaggcc tccgagctgc gcagcgtgaa
     9601 agtggcgaag gatcccggct gcgcagtgtg cgcgtagcgc acgccgccgc gtgccgctgt
     9661 actacactgg cgcggtgaac gctccagagc aagccgaggc gcagttcgac cgcgcggcgc
     9721 aggacgtggg caacctcgtc ctgctcgagc acttcaacgt cacgattgcc gaccagcggc
     9781 tggcgacgct gttctacgtc gtcggcctgg gcggcacgcg cgatccgtac ctcttcaccg
     9841 ggttcaccaa catgtgggtc aacttcggcc gcacgcaggc gcacctgccg agccgcggcg
     9901 cgcagccgcg cgcgcaggtg ctgcgcggca gcgcgggctt cgtggtgccg gatctggaag
     9961 cgctgaagaa gcggctcgat ttcgccggcg ccgagatgaa gcgcgtggtg cccgaggcgg
    10021 cgacgaagtt ttcctggcgc gagcagggcg ggtgcatcga ggcgacctgc ccctggggca
    10081 accgcatccg ctgccacgcc cccggcccgg agttcgggcg gaccgagctc ggcctcgtgt
    10141 acgtggattt cgatgtgccg cccggcacgg ccgccggcat cgcacgcttt tacgccgagg
    10201 tgatgcgggc gccggcgagc gccgagggcg gccgcgccac ggtgcgcatc gggcgcgggc
    10261 agcgcctgct gttcacggag tcgagcgagc cggtcgcggc gtacgacggg caccacgtgc
    10321 aggtctacat cgccgacttt tccagcccct acgcgtggct gaaggcgcgt ggcctcgtca
    10381 cgatggagag cgacgcgcac gagtggcgct tccagcgcat cgtcgatccg cagtcgctcg
    10441 agccgctatt cgagatcgag cacgaggtgc gcagcatgac gcatccgctc tacggcaggc
    10501 cgctggtcaa tcgcaatcac gccgtgacga acgtcaacta ccgaccgggt caggacgcct
    10561 ttcgcggcac ctactgaagg ttcgtcgacc ggggcggcgc accagcgccg ccccgggcgc
    10621 gctcacttgc tgccgatgcc gatgtgtacc gggttggaaa gctgcgccac gccgccggcg
    10681 ccgcgctcgc gcagcagccg gtccagagcg ccgcgcgccg tgtcgatctc gtccgcggtc
    10741 agattgaggc tgccgagcac cgcgtcgacg atcgggttgc tgcacacgat ccagtcccac
    10801 agcgcttcgc cgtcccggaa cgcggtggtc tcggtgacgg tctcgacgcg cacgcccttc
    10861 agcccggcgc cggcgagttc ggcgcgcagc cgcgccggat cggcgagctg aaaaggcagc
    10921 ggcggcggat ccatcggcgg cccggcgaac cccgggcgca ccgactgcag cgcgccgacg
    10981 aggaagccga gaaagtcgat ccggtgtgga tcgccgtagg cgtgcaccag cacgcgcccc
    11041 cccggcttca ccgcgcgaac catctcgcgg atgccctgcg gcatgtcggg aaacagcatg
    11101 acgccgaact gcgagcccgc cagatcgaac tgattctcgg cgagcgccag cgcgtgcccg
    11161 tccatcaccc gcgtttcgat ttccagcttc tccttgcgcg cgcgcgcttg cagcagctca
    11221 agcatcgccg gcgactggtc caccgccatc acctgcgcgc cgagacgcgc cgccggaagg
    11281 ctgagcgcgc cgctgcccgc ggcgacgtcg agaaagctca ttccggcgtg cagcccggct
    11341 cggcgcaggc cttcgcccgc gagccacatc tgcgtcggtg tgttggtccg gtcgtagccg
    11401 ggcgcgatcg cgtcccaggc cgcgcgcgtg tcctcgcgca gcgcggcttg cagcgcccgc
    11461 acgaacgcct cgggctgctc catgggcgga tcgtcggcgg cgcgctcgat gacgtgcagc
    11521 ggccagccga agcgccggct tgcgcgctcc gcgacgccga gcggcgtggc gaggtcgtgc
    11581 cggccccaga tgagggtcgt cggcacgccg atgcgcgcga gctcggcctc ggggatcgcg
    11641 ggcatgccga attgctgcat caggccgccc gtcgccgcct gcacgcccgg cgtgcgcgcg
    11701 cgatcgaggt tgtacgcccg gagcgcgtgc cagcgctcgc ccatgcgctc gcgcatggca
    11761 tccaggtcgt acgcgcagcg cgcccacagg cggtcgtgat tctcttcgct cggctgcgcc
    11821 gagaattcca tgagcgcgtg gccgaactcc ggcgccggct ggaacggcgc cagcccgagc
    11881 gcgtcggcaa gcaccagcgc cttcagccgc gcgccgtgcc gcgcggcata gcgcgcggcg
    11941 atggcgccgc cgacgatctg gccgacgagc accggcggct cgctgcaggt gcgctcgatc
    12001 agcgcatcga gccaggcgag cacgccttcg acgtcgagcg cgccgccttc gatgttcgag
    12061 gcgccgtggc cgggcaggtc cggcgcgatg acgcgatggc gctttgccag gtccggcagg
    12121 acgcgcagcc acttcgtcgc gtgctcgccc gggccgtgca gcaggacgag cggcgctccg
    12181 gtgccaccct ccagcacggc ggtggcgaca ccggcgagcg tcaagcgcct ttcggcgaca
    12241 ggcaagccgg caagcagcgc ttcgcgggcg tcgccggcgg gcgcagctgg gatctttgca
    12301 gtcgttcttg caaccatggt gtgcctcctt ctcgtgggtt ggggtcgcaa cccgacgtta
    12361 ggtccgcagg cacgggcggc gcttcggcag aagtccgcag ccacggcgcg ccgcggcatg
    12421 ggtgatttcc cccatagcgc gcgcaccgcc ggctcagatc aacttcttct cgtacgcgta
    12481 ggccaccgcc gcggcgcgcg aaggcacgtc cagcttggtg aggatgttgc tcaggtggcg
    12541 gtcgaccgtt ttctcgctca actcgagcgc agcggcgatg cccttgttgg tcttgcccgt
    12601 ggcgacgagg cgcagcacct gcagctcgcg cgcggtgaga ttcggcccgc ggccgcgcgc
    12661 gggcgcttcg agccgtgcca ggtcgggtgc cgctccgagg cgcgcgaact cgccgcgcgc
    12721 agccgcgatc tcgagcgcgc cgccgtcttc gtcgccaagc gcgcggcacg ccgcgccgag
    12781 cagcgcgcgt acgcgcgccg cctcgtatgg atcgccgagc tgccgccacg cctgcgccgc
    12841 atgacgcagc ggcgcgaggg cctgccgcgg cgcgccggcc gcaagctcga gcgcgccgcg
    12901 cgcctg
//
LOCUS       DBNHKE010000041         5024 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1010633, whole genome shotgun sequence.
ACCESSION   DBNHKE010000041 DBNHKE010000000
VERSION     DBNHKE010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1010633"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="AC16O9_02345"
     CDS             <1..839
                     /locus_tag="AC16O9_02345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6515489.1"
                     /translation="LAPRDRTWQLEITLRGTGKTLLARGAALPAATGGGYVLVFDDIT
                     QLIQAQRATAWAEVARRLAHEIKNPLTPIQLSAERLDMKLADRLDGEDAETLRRNTQT
                     IVNQVTALKSMVDDFGDYARLPAPVPAGLDLNGLVADVLALYENSRVPITKRLAPGLP
                     PVWADTAQIRQVIHNLVQNAQDALENRKEPLQQPAIEVCTELVGDKVRLSVADNGDGF
                     PEQLMARIFEPYVTTKPRGTGLGLAIVKKIVDEHHGSIAIENRSERGASVSVLLPLAE
                     AA"
     gene            847..1192
                     /locus_tag="AC16O9_02350"
                     /pseudo
     CDS             847..1192
                     /locus_tag="AC16O9_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935896.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1306..1593
                     /locus_tag="AC16O9_02355"
                     /pseudo
     CDS             1306..1593
                     /locus_tag="AC16O9_02355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
     gene            1666..2094
                     /locus_tag="AC16O9_02360"
                     /pseudo
     CDS             1666..2094
                     /locus_tag="AC16O9_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
     gene            2138..3511
                     /gene="trkA"
                     /locus_tag="AC16O9_02365"
     CDS             2138..3511
                     /gene="trkA"
                     /locus_tag="AC16O9_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR6515490.1"
                     /translation="MKIVILGAGQVGSSVAESLVSEQNDITVVDVDAARLRGLQERFD
                     LRTVAGSASHPSVLSDAGIDDADLLIAVTQSDETNLVACKLAARMFNVPRRVARIRAN
                     AFLDNKMLLGADGFDVDLAICPEQVLTDYIVKLVEFPEALQVLDFADGKVSLVAVRAY
                     QGGPMVGHPVKEIRAHMPNIDTRIVAIFRHDRAILPDGDTVIEADDEVFCLAAAANIS
                     KVMRELRRMDQPVKRVMIAGGGNIGMRLARALERDYSVRLIEHNKRRSEFLAAKMNQA
                     LVLNGDATDEELLEQENIAEMDLFVAVTNDDENNIMSCLLAKRMGARRVVALINRRSY
                     VDLLQAGQIDIAISPAQATIGVLLAHVRRGDVVAVHSLRRGAAEALEAVVHGDRESCR
                     VTGRTVQEIDLPAGATIGAVVRNGEVLMAHHDTRIEAEDHVIVFVTDKKTLPRVEKLF
                     QVAARFL"
     gene            3585..4979
                     /locus_tag="AC16O9_02370"
     CDS             3585..4979
                     /locus_tag="AC16O9_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR6515491.1"
                     /translation="MLVPLAFSLALGDGAHWAYVEALAATLAAGIALWLATFRVEREL
                     RVRDGFLLVALVWTVLPAFATLPLLLQLSELSFTDAYFEAVSGLTASGATVLSGLDRL
                     PASLNLWRGFMIWIGGMGVIVLAVAILPLLGVGGSQLFKAETPTPMKDTKLTPRIAET
                     AKGLWLVYFGLTVACGLAYYWAGMNWLDALMHAFTTLGLGGFSTHDQSYAYWNSPRIE
                     AVAIVFMLIAAINFATHFLAWHRRSLGVYRVDPEAKVFLGVVAVSVLGIAMYLVWAQV
                     YPDFGSALRFAAFNVVSIATTTGYANADFNQWPIFAPLWMLFLCSFASCSGSTGSGMK
                     MIRAQILFKQIFRELLRIIHPRVTNPVKLGGQLVENNVVFAVLAYVFVYVSCIIVFTL
                     LLTLSGSDVVTAFSAAVVSLNNTGPGLAQVGPASNYAVLNDFQTWVCSFGMLLGRLEL
                     FTLLVVLTPAFWRK"
BASE COUNT          751 a         1684 c         1758 g          831 t
ORIGIN      
        1 ttctcgcgcc ccgcgaccgc acctggcagc tggagatcac gctacgcggc accggcaaga
       61 cgctgctggc gcgcggcgcg gcgctgccgg cggcgaccgg cggcggctac gtgctcgtgt
      121 tcgacgacat cacgcagctg atccaggcgc agcgcgcgac ggcctgggcc gaagtcgcgc
      181 ggcgcctcgc gcacgagatc aagaaccctc tgacgccgat ccagctctcg gccgagcgcc
      241 tggatatgaa gctcgccgat cggctcgacg gcgaggatgc ggagacgctg cggcgcaaca
      301 cgcagaccat cgtcaaccag gtgaccgcgc tcaagtccat ggtggacgat ttcggcgact
      361 atgcgcgcct gcctgccccg gtgcccgcag gtctggactt gaacgggctg gtcgccgatg
      421 tgctggcgct gtacgagaac tcgcgcgtgc cgatcaccaa gcgccttgcg ccggggctgc
      481 cgccggtctg ggccgacacc gcgcagatcc gccaggtcat ccacaatttg gtgcaaaacg
      541 cgcaggatgc gctggagaac cgcaaggagc cgctgcagca gccggcgatc gaggtatgca
      601 cggagctcgt cggcgacaag gtgcggctgt ccgtggccga caacggcgac ggcttccccg
      661 agcagctgat ggcgcgtatt ttcgagccct atgtcaccac caagccgcgc ggcacggggc
      721 tgggtctcgc catcgtgaag aagatcgtcg acgagcacca cggctcgatc gccatcgaga
      781 accgctcgga gcgcggcgct tcggtgagcg tgctgctgcc gctcgcggaa gcggcctgag
      841 agcgtcatgg cgcagatcct ggtggtggac gatgaaatgg ggatccgaga attgctctcg
      901 gagatcctgg aagacgaggg gcacgccgtg ctgctcgccg agagcgcgag cgcggcgcgc
      961 agcgtgcgcg aagccaagcg cccggacctg gtgctgctcg acatctggat gcccgatacc
     1021 gacggcatta cgctgctcaa ggaatgggcg agcagcgggc agctgaccat gccggtggtg
     1081 atgatgtccg ggcacggcac catcgagacc gcggtcgagg cgacgcgcgt cggcgcgctc
     1141 gactatctgg agaagcccat cgcgctgcag cgcctgctca gtaccgtgaa acgcgcgctg
     1201 cgcgaccatg atctggcggc gcctcgcccg ttgtcgctga cggcgctggg gcgctcggcc
     1261 tcgctggtgg aactgcgcaa gcgcctggca cagctggcgc agacggcggc cccgctcatg
     1321 ctgcgcggcg aacccggcat gctgcccgag atcttcgcgc gccagctggc cgcgccgggc
     1381 gccccgtttc tcgccggcgc cgagcagctg gccgacgcct ccagcgagct gctgtcgcgg
     1441 gcggcgggcg gtatcctgtt cctgcccgac ctgtcgcgac tgcagcgcgg cgagcagaag
     1501 aacctcgagt tcctgctggc gcgcgccgac aagcaccgcg cgcgcatcgt ctcgttctcg
     1561 ccgctcgatg ccagggcgct cgtggagcgc cacggctacg acccggcgct cgttgcccgg
     1621 ctgggtgagc ttacgctgcg cctgcccgcg ctgcgcgagt tcgccgagga tgtgcccgac
     1681 ttggcggcgc aaatgctggc gcagctcgtg gaagcgcgcg tgtgcccacc gcgacgcctg
     1741 tcgacggggg cgctgaacgc gcttcggcac caccactggc cgggaaacct ggccgagctg
     1801 gagcacgcgg tgaagaacct cgcgctgagc gcgctcgagg acgaggtaag cgtcgaggat
     1861 gccgagcgcg tgctggccgc gcaggcgcca gcggcggccg ccccggagct cgcgctcgac
     1921 cgaccctatc gcgaggcgcg cgaagcgttc gagcgcgtgt atttcgagaa cctgctggcc
     1981 gccgcgagcg gcagcatgtc gcgcgtggcc gagaaaagcg gcctggagcg cacccacctg
     2041 taccgcaagc tcaaggcgct cggcctgccg gtcggccggc gcgaggaagg ttgaaacgga
     2101 atagaatgcc gcgcttctcc ctaggagcgg tagcggcgtg aagatcgtca ttctcggcgc
     2161 cggccaggtc ggcagctcgg tggccgagag cctggtatcc gagcagaacg acatcaccgt
     2221 ggtggacgtg gatgcggcgc gcctgcgcgg gctgcaggag cgcttcgacc tgcgcaccgt
     2281 cgccggcagc gcctcgcacc cctcggtatt gagcgacgcg ggcatcgacg acgccgacct
     2341 gctgatcgcg gtgacgcagt cggacgagac caatctcgtc gcctgcaagc tggcggcgcg
     2401 catgttcaac gtgccgcgcc gcgtggcgcg catccgcgcc aatgcgttcc tggacaacaa
     2461 gatgctgctt ggtgccgacg gcttcgacgt cgacctcgcg atctgccccg agcaggtgct
     2521 caccgactat atcgtcaagc tggtcgagtt cccggaagcc ctgcaggtgc tcgacttcgc
     2581 cgacggcaag gtgagcctcg tggcggtgcg tgcctatcag ggcgggccga tggtggggca
     2641 cccggtgaag gagatccgcg cgcacatgcc gaacatcgac acgcgcatcg tcgccatctt
     2701 ccgccacgac cgcgccatcc tgcccgacgg cgacaccgtg atcgaggcgg atgacgaggt
     2761 gttttgcctg gcggcggcgg cgaacatctc caaggtgatg cgcgagctgc gccgcatgga
     2821 ccagccggtc aagcgcgtga tgattgcggg cggcggcaat atcggcatgc gcctcgcgcg
     2881 cgcgctggag cgcgactatt cggtgcgcct catcgagcac aacaagcgcc gcagcgagtt
     2941 ccttgccgcc aagatgaatc aggcgctggt gctgaacggc gacgccacgg acgaggagct
     3001 gctcgagcag gagaatatcg ccgagatgga cctgttcgtg gcggtgacca acgacgacga
     3061 gaacaacatc atgagctgcc tgctcgccaa gcgcatgggc gcgcggcgcg tggtggcgct
     3121 catcaaccgg cgcagctacg tcgacctgct gcaggcgggc cagatcgaca ttgccatctc
     3181 gcctgcgcag gccaccatcg gcgtgctgct cgcgcacgtg cgccgcggcg acgtggtcgc
     3241 ggtgcacagc ctgcggcgcg gcgcggcgga ggcgctggag gcggtggtgc acggcgaccg
     3301 cgaatcctgc cgcgtcaccg ggcgcaccgt gcaggagatc gacctgcccg ccggcgccac
     3361 catcggcgcg gtcgtgcgca acggcgaggt gctcatggcg caccacgaca cgcggatcga
     3421 agccgaggat cacgtcatcg tgttcgtcac cgacaagaag accctgccgc gggtggagaa
     3481 gctgttccag gtggccgcgc gcttcctcta ggccgcatcg cgtcgtgcgc ccgctcgccg
     3541 tcctgcatct gctgtcgctg gtgctggtgc tgttctcggg ctgcatgctg gtgccgctcg
     3601 cgttttcgct tgccttgggc gacggcgcgc actgggccta cgtcgaggcg ctggccgcaa
     3661 ctctggcggc gggcatcgcg ctgtggctgg cgacgtttcg cgtcgagcgc gagctgcggg
     3721 tgcgcgatgg cttcctgctg gttgcgctgg tatggacggt gctgccggcg tttgccacgc
     3781 tgccgctgct gctgcaatta agcgagctgt cgttcaccga cgcgtacttc gaggcggtct
     3841 cggggctcac cgcgagcggt gccacggtgc tctcgggcct cgatcgcctg ccggcctcgc
     3901 tcaacctctg gcgcggcttc atgatctgga ttggcggcat gggcgtgatc gtgctggcgg
     3961 tggcgatcct gccgctgctt ggcgtgggcg gctcgcagct gttcaaggcg gagacgccga
     4021 cgccgatgaa ggacaccaag ctcacgccgc gcattgccga gacggcaaag ggcctgtggc
     4081 tggtgtattt cggcctcacg gtcgcctgcg gcctcgccta ctactgggcc ggcatgaact
     4141 ggctcgatgc gctgatgcac gccttcacca cgctcggcct ggggggcttc tccacccacg
     4201 atcagagcta cgcttactgg aactcgccgc gcatcgaggc ggtggcgatc gtcttcatgc
     4261 tgatcgcggc gataaacttt gccacccatt ttctcgcctg gcaccggcgc tcgctcggcg
     4321 tgtaccgcgt ggacccggaa gcgaaagtct tcctgggggt ggtcgcggtc tcggtcctcg
     4381 gcatcgcgat gtacctggtc tgggcgcagg tctaccccga ctttggcagc gccctgcgct
     4441 ttgccgcgtt caacgtggtg tcgattgcca ccaccaccgg ctacgccaac gccgacttca
     4501 accagtggcc gatctttgcg ccgttgtgga tgctgttcct gtgcagcttc gccagctgct
     4561 cgggctcgac cggcagcggc atgaagatga tccgcgcgca gatcctgttc aagcagatct
     4621 ttcgcgagct gctgcgcatc atccacccgc gcgtcaccaa cccggtgaag ctcggcggcc
     4681 agctggtgga aaacaacgtg gtgttcgcgg tccttgccta cgtgttcgtg tacgtgtcct
     4741 gcatcatcgt ctttacgctg ctgctcacgc tgagcggatc cgacgtggtg accgcgttct
     4801 cggcggccgt ggtctcgctc aacaacaccg gcccggggct ggcgcaggtc gggccggcct
     4861 cgaactacgc ggtgctgaac gacttccaaa cctgggtgtg ctcgttcggc atgctgctgg
     4921 ggcgcctgga gctgtttacg ctgctggtgg tgctcacgcc ggcgttttgg cgcaagtgag
     4981 ccgcgtgccc gttcgcatcg cgcccgtcgg cgcggccgcg cccg
//
LOCUS       DBNHKE010000042        34343 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_270309, whole genome shotgun sequence.
ACCESSION   DBNHKE010000042 DBNHKE010000000
VERSION     DBNHKE010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 34343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34343
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_270309"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC16O9_02375"
     CDS             complement(<1..663)
                     /locus_tag="AC16O9_02375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR6515492.1"
                     /translation="MRAAPAGRFARGLLWRVVRSGVEPSHVFGTLHAGDPRLEPLPAA
                     VQDAFGQARSLMLEYLADPYGRERFLEAAMYLDGRTLPQDIGDEHFERAVSAMQPIGL
                     PREFVSKLKPWGVLLNLRISRQPAGAMAPDAQLFALARERRLPVSQIENVEEQVFTFD
                     EMSRDSQVALLRHFLEHRDALDALAERSLHAYLARDLQGLWDAQQDYAHRHPEIAPHH
                     AEL"
     gene            complement(708..2102)
                     /gene="fumC"
                     /locus_tag="AC16O9_02380"
     CDS             complement(708..2102)
                     /gene="fumC"
                     /locus_tag="AC16O9_02380"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR6515493.1"
                     /translation="MATRKEKDSFGPIEVPADRLWGAQTERSRRLFRISGERMPLAVI
                     HALAQVKRSAARVNRALGLLEPNIADAVERAADEVLAGRHDEEFPLVVWQTGSGTQSN
                     MNVNEVLANRASELLGGERGPRRLVHPNDHVNLGQSSNDAFPTAMHIAAARAVEHDLL
                     PRLERLEATLATKAEAFAGIVKIGRTHLQDATPLTLGQEFSGYVEQLAQAEAAIELTL
                     PGLCELAIGGTAVGTGLNTHAEFGDRMAKDLSQATGMTFEAAPNKFAALAGHEPLVFA
                     HGALKTLATALHKIANDIRWLASGPRSGLGEISLPENEPGSSIMPGKVNPTQAEALTM
                     LCVQVLANDVALGIGGASGQFELNVYKPMLIHNFLQSVRLLADGCASFEEHCARGIEA
                     NAARIAELVERSLMLVTALAPHIGYDRAAEIAKKAHREGSTLKEAALALGYVSAEDFE
                     RWVRPEAMTRPGAG"
     gene            complement(2120..2503)
                     /gene="trxA"
                     /locus_tag="AC16O9_02385"
     CDS             complement(2120..2503)
                     /gene="trxA"
                     /locus_tag="AC16O9_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR6515494.1"
                     /translation="MATVELTKDNFEKVITESPIVIVDFWAPWCGPCRGFAPIYEQAA
                     ESHADVVFGKINTDQEQELAGAFNIRSIPTLMVFREKVVLFQQAGALPASALDQVITQ
                     AKALDMAKVHQEIAERDRRDGQAPS"
     gene            2502..3392
                     /gene="gluQRS"
                     /locus_tag="AC16O9_02390"
     CDS             2502..3392
                     /gene="gluQRS"
                     /locus_tag="AC16O9_02390"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR6515495.1"
                     /translation="MRIPVEKRAVYSLGVMADYVGRFAPSPTGPLHFGSLVAALASYL
                     EARAANGRWLLRMEDLDRARAQPGAADAILRALERLGFEWDGPVLMQSARLERYREVL
                     DELSRRGLAYPCSCTRRELEDSALAIDGARVYPGTCRHGLAPGKAARALRLRTHGAPI
                     GFADAIQGWVEQPVEQAVGDFVLLRADGVIAYQLAVVVDDLDQGVTHVVRGADLLDST
                     ARQIHLQRLLGAYTPCYAHVPVALNAAGEKLSKQTAARPLDLSDPQAELARARRFLGQ
                     GASGWDLARVPRTRAISAPE"
     gene            complement(3373..4272)
                     /locus_tag="AC16O9_02395"
     CDS             complement(3373..4272)
                     /locus_tag="AC16O9_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017807.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6515496.1"
                     /translation="MSPAPAPEHLPRHLWWVLLGITLVWGFNWTAMKVAISEVAPFTF
                     RTFCLAAGSGVLFAVLRATGQPLAIPRSQWRRLALIAFFTITCWNVLVVFGLRLIPSG
                     RAAILAYTMPAWAIPLSVWFLRERMSARKLAGLALGMTGMALLLVQEFTSLRGAPLGA
                     LLVLGAACTWAIGTLLQKKFPVHAPVAPLTAWMMLIGGVPIYVGAMLFDDFSKLQDIS
                     LWPALAVAYNVLLSFAWAHWAWIKLATSVSVTTFSLSMLMTPVVGVFSGMLFLGERPG
                     WLEFSALALVLASLASVVIPARR"
     gene            complement(4269..5150)
                     /locus_tag="AC16O9_02400"
     CDS             complement(4269..5150)
                     /locus_tag="AC16O9_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6515497.1"
                     /translation="MSPAPDPVQRFAWVFPGLFVVLWSTGFIGAKLGLPYVEPATFLV
                     IRFVLVLVVLLPLCVAMRAPWPTSGVRFMHMAFAGALIQGGYLGGVFLSLHQGMPAGA
                     SALITALQPILAAVLSGWLLGERTNARQWLGLALGIAGVALVVREKWVLEGVGLAATA
                     LSVLALVSISLGAVWQKRHCASVDLRTGAAIQFGAALLVLTPYALLFESRPVVWSAEL
                     VVALVWLVFALSLGAVLLLFWLIRHGAATKVASLMYLVPPCTALVAWPLFGETYTAFA
                     AAGMALAMLAVWLVSRA"
     gene            5388..5930
                     /locus_tag="AC16O9_02405"
     CDS             5388..5930
                     /locus_tag="AC16O9_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR6515498.1"
                     /translation="MYVTPEQIQATGKANVETMLSLAATQFAAIEKLATLNANAVKTA
                     FEDSLSNTRALFGAKDVQEFVTLQNTLATPAIEKAIAYSKSVYEVATETNAELSKMAE
                     QRVAEWNENFSSMLDKAVKNAPAGSDVAVAAVKSMIAAANSAYDNMNKVAKQATEIAE
                     ANVNAASETVKGLTKAKKTA"
     gene            complement(6036..8435)
                     /gene="lon"
                     /locus_tag="AC16O9_02410"
     CDS             complement(6036..8435)
                     /gene="lon"
                     /locus_tag="AC16O9_02410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6515499.1"
                     /translation="MEFWRRKRQETLEGGGGTGAEAPRGRAGRGTALAVRPIPEDALI
                     LVPLRSAVLFPGIITPLAVGRGSSVAAVQEAARQGRKLGLLLQRDPKTNQVGADDLHW
                     VGTAGEIVRYVPAAEGVHHLAVQGQTRFRVTEFLEGWPFLVGRVVWVPEATEGGEEIE
                     ARFLQLKAQAVEAIKLLPNVPDELAGAVQAIDSAPQLADMVANLLDIENEEKQAVLEA
                     FELKPRLDLVLAHLAERVSVLRLSKEIGDKTKKEFDERQREHVLREQMRQIQKELGEG
                     EDTAAELEELQKSLEAAGMPEETLRHAKKEFKRLSRMGEGSGEASMLRTWLELMAELP
                     WKAEPQLAVDIAEARRVLDEDHHGLEKVKRRILEYLAVRKLNPEGRSPILCFVGPPGV
                     GKTSLGQSIARATGRKFQRVALGGLHDEAEIRGHRRTYIGALPGNVIQALKRAESRGG
                     VLMMDEIDKLGMGGFHGDPSSALLEVLDPEQNAKFRDNYLGVDFDLSRVMFIATANVL
                     DTIPGPLRDRMEVIQLPGYTEEEKLQIARRYLVKKQLEANGLKAEQAALTEGAIREII
                     ASYTREAGVRNLEREIGAVLRHVAMCIAEGNAQCVTVGAADLQPILGARKFENEVALR
                     TSLPGVATGLAWTPVGGDILFIEASKVPGSGKLILTGQLGDVMKESAQAALTLAKTWS
                     GDALEKTDIHVHVPAGATPKDGPSAGVAMFVALTSLLQGKPVRSDVAMTGEISLRGLV
                     LPIGGVKEKTLAALRAGLSTVMLPRRNEKDLEDVPAEARKKLRFVFLDKVEDAVQAAL
                     Q"
     gene            complement(8550..9875)
                     /locus_tag="AC16O9_02415"
     CDS             complement(8550..9875)
                     /locus_tag="AC16O9_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6515500.1"
                     /translation="MDPHFLTGLVGLASLIALIAIRVPIAHAMLVVGAIGIAILSGPQ
                     ILLSQLKTLVYSQFSIYDLSVLPMFVLMGNIASRAGLSRDLFRAANAWLGWMRGGVAM
                     SAIAACGGFGAVCGSSLATASTMGQIALPELRRYRYSPELATGTLAAGGVLGILIPPS
                     VVLVVYAIVVEASIVDMFIAAFIPGLIAIAMFLLTIAIYVRVRPDAGPPGDRVTREEF
                     IAATIGVGPVLAIFGAVIGGIYFGLFNPTPAAAVGVFLVTVLAFARRSVNLQGMKAAL
                     LETAKTAGMIYLILLGAQLLNIFMARGGVPQAAAEVMATSGLAPLEVLVLLLVALIAL
                     GCLMDSLSMILLAIPFFWPVISGLDFGMSVEELKIWFGILALIVVELGLITPPVGMNV
                     FVINSLARDVPMSRTFLGVMPFFGAEIVRVALLVAFPGLVLWLPKVLAG"
     gene            complement(9886..10395)
                     /locus_tag="AC16O9_02420"
     CDS             complement(9886..10395)
                     /locus_tag="AC16O9_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6515501.1"
                     /translation="MDEGPARARAGPSGLARRAIEWWALAGGVVLLAVALMTTWSAFS
                     GWVFGKPLPGDFELTEILVAVAVFAFLPYCQQTDANVTADLFTARAGPRALAAFRLFG
                     GLLALVVALVLAWRTWAGLLDYRKYVETTAILKIPIWWAYVPALASLALLVLACAIVL
                     KDEVRALRG"
     gene            complement(10399..11334)
                     /locus_tag="AC16O9_02425"
     CDS             complement(10399..11334)
                     /locus_tag="AC16O9_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6515502.1"
                     /translation="MPPKAPAHTKMLAPWAADVEKASGGKVKIELFPSMALGGKPPEL
                     INQVRDGVVDIVWTVNGYTAGLFPRSEVFELPFVHTNNATATNLAMREMFLNGAIAPD
                     YESVKVMFLHVHAGQAIHMAEKEVRTPDDLKGLKMRIPTRTGAWIIEALGAAPASMPV
                     PALPQALSKKVVDGAFIPWEIIPPLKLQDVTKVQVEGPNRMRFGTTTFQVSMNKNTWA
                     KLPRDVQKAFEANNGEARWRTVGKVWTDSEAGGIAVAKKAGNKHIQLTEAELAMFKAK
                     LEPVVDRWIAEVDSKGIDGKALVDQARARISKHSK"
     gene            11611..13137
                     /gene="gshA"
                     /locus_tag="AC16O9_02430"
     CDS             11611..13137
                     /gene="gshA"
                     /locus_tag="AC16O9_02430"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR6515503.1"
                     /translation="MLQERLRALTRDTLRGMRRGIEKESLRTRPDGALALTPHAAALG
                     SALAHPHITTDFSESQLELITGTHLTVEGCLDELTRIHQYVVRVIGEELLWAGSMPCG
                     LPAEHEIPIGRYGRSNIGRAKTVYRNGLSWRYGRRMQTISGIHYNWSLPGLGNAEYFA
                     LIRNFRRHGWLLLYLFGASPAACASFVEGRPHPLRELRPGTLYLPHATSLRMGRLGYQ
                     SDAQSSLMVVCNDLESYGATLQRGLTQVYPAYERIGIREPGAQGDDPDSYRQLSTTLL
                     QIENEFYGKIRPKRPIQQGERPLHALRERGVEYVEVRLMDLDPYAAVGITAPVMRFLD
                     VFLLHCLLSESPPDTVDEVACVGRNQECVAARGREPGLRIGCGPEEIGLQKWGEQLLT
                     ECAPVAEALDAALGGQLYREAHAAQAAAIRDPALTPSARILADMHRYGDSYRDFALAQ
                     SRRHREVLLALPFPAAEEERFRHLAAASIAEQKAIEAADRLPFEEYRKNYLAVERLGV
                     "
     gene            13156..13512
                     /locus_tag="AC16O9_02435"
     CDS             13156..13512
                     /locus_tag="AC16O9_02435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6515504.1"
                     /translation="MPTTTASGLIYEEKVVGTGDTAQSGQDVSVHYTGWLLYGGERGK
                     KFDSSKDRGEAFGFALGAGQVIKGWDEGVQGMKVGGTRVLTIPPALGYGARGAGGVIP
                     PNATLVFEVELLGIEK"
     gene            13591..15042
                     /locus_tag="AC16O9_02440"
     CDS             13591..15042
                     /locus_tag="AC16O9_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515505.1"
                     /translation="MADSPVWMIWPGNAALSALVWFVIAMPFLYAARAPVHGLLRALG
                     LLAGGPLRLAARALLASAQELRERNRAVLFAQGREEAGERIAREFERVAALVKRDLEG
                     YPALQRKLLDEITRVEEDYRKCGEVPPPPPDWVEAVAAVAKIKPNGNELVQRLLEEIN
                     VSIKAIHDKTIAEYRRAYQARHRILDGFMPFWRSLDKTLARADRRMTDLAERATAIDG
                     QMERYEAIAKKADKAEHALTVSAATQFFIATLVLAIAAGGALINFKLIALPMSEMVGA
                     GDYLTASLRTSEVAALVIIFVEATMGLFLMETLRITHLFPRIHNLGETMRRRMMWASF
                     TLLLILAGIEAALALMRDMLIADKQALLQSLAATQAVASDGWLSGIPTAGQMLLGFIL
                     PFALAFVAIPLESFINSARTVGGAALVVLARSAAVVLRVAGNFLRQLCKVLITLYDIT
                     IVVPLLAERWVKGMRPPGLGQGARGDLKAGGQS"
     gene            15039..15698
                     /locus_tag="AC16O9_02445"
     CDS             15039..15698
                     /locus_tag="AC16O9_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6515506.1"
                     /translation="MKRRAFLVSLPLLGAAQLLGCTDPRSHAHAVYLLVDTSGTYAEE
                     VGKAQLIVNYLLGALQPGDSLAVARVRSRSFSEKDIIAKATFDQRPSQANAQKRLFKD
                     RVEAFTSTARGSVHTDITGGLIQGALFLNETGAGKKTILIFSDMQEELDRATVRDFPI
                     DLKDIRVVALNVVKLKTDNLDPRRYLGRLEDWQKRVKSAGASEWRVINDLEHLDAMLK
                     S"
     gene            complement(15718..16197)
                     /locus_tag="AC16O9_02450"
     CDS             complement(15718..16197)
                     /locus_tag="AC16O9_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019416.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HIR6515507.1"
                     /translation="MNASGARFSACRRWRTLLWRCWDDARPVVNFLMLNPSTADETVL
                     DPTCTRARDYAERWGYGALLVTNVFAWRATDPVEMRAAPDPVGRGNDAAILRAAKAAQ
                     LVVCAWGNHGLYLGRSEAVQRLLRGAGVALHVLRLNANGEPAHPLYLPGRFRPRRWR"
     gene            complement(16194..17408)
                     /locus_tag="AC16O9_02455"
     CDS             complement(16194..17408)
                     /locus_tag="AC16O9_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515508.1"
                     /translation="MPPSRRALSLGVIALTLALAYGVWYSYSVVLVALLKEFGWSRSV
                     LAGAFSVFTLVHGAVNPVVGALCDRLRPMRLMAAGGVALGLALWANSAIAAPWHLYLS
                     FGVFTAIAVAAAGWVPALVQVQRDFQDRLGLALGIVSSGVGVGMLLVVPLAQLLIDAY
                     GWRTAFRVLGAICVLWIVPSSLYLHLRSPVRRPVPEPAAATAKPLPVITLAQAMRGAP
                     FWLMLAAFFFGNLCSQTLHVHQVAYLVDQGVSALVAASVVGVIGVSSIVGKTGGGWLS
                     DRMEREVVYVAGIAIMLAAVAALVALAGAPAAWAIYGYAVLLGVGYSATASLTPAMVS
                     DRFGGPHFGTIVGVGLMGAAVGSALGPWMAGLLFDRTGSYMLAFAIAAGCGLAAGAAA
                     WWARVLRVRAAE"
     gene            complement(17427..17720)
                     /locus_tag="AC16O9_02460"
     CDS             complement(17427..17720)
                     /locus_tag="AC16O9_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6515509.1"
                     /translation="MAKAYMVVTYRSLKDPKALEAYAKLAGPAVQAEGGRFLVRGMPT
                     KTYELGMNQRTVVVEFDSVEKARAAYEGPAYKKALDALGNAAERDMRLVESAA"
     gene            17848..18639
                     /locus_tag="AC16O9_02465"
     CDS             17848..18639
                     /locus_tag="AC16O9_02465"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR6515510.1"
                     /translation="MSTIPNHALRQLRAGKLAIGLGLRQARTVDVGQILKTAGFDWMF
                     IDCEHNSMGTDTAAQIASAALAIGITPVVRVAGKEHWLASRMLDNGAQGVVVPHVDTE
                     QEAKRIADHCRFPPIGHRSMGGGLQQLGFASMPVGEAANIVNDETLVVVMLESPKGIE
                     NCEAIAAVKGIDALLIGTNDLCFEMGIPGQFDDPRVAEAYKKVIAACKKHGKFAGMGG
                     MYTKELLERHIAMGVQLILSGSDFSLLQQAAKARADLVRGFEGKA"
     gene            18636..19364
                     /locus_tag="AC16O9_02470"
     CDS             18636..19364
                     /locus_tag="AC16O9_02470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6515511.1"
                     /translation="MTPHIATVVVSGSPMEVFLFAPAGAGPHPGLVLCQHIPVGHTGV
                     ENDVVTLKTAERYCENGYFVAVPFIFHWWPKSADIELKRAEFRDDWTRLDLDAAFELL
                     ATQPGVDKSRVGIVGHCWGGRVAWLGACTNPRYKAAAIFYGGRVKLVMGKGNPPAIDL
                     TPGMRCPVIGFFGNEDTNPTPADVDDYEAALAKAGVEHVFHRYDGAGHAFQSFNNPER
                     YRKEASEDAWNKVLAFFGGKLGRT"
     gene            complement(19371..20144)
                     /locus_tag="AC16O9_02475"
     CDS             complement(19371..20144)
                     /locus_tag="AC16O9_02475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515512.1"
                     /translation="MSDIASRFRLDGRVALVTGAGRGIGRSCALALSQAGAEVWLMAR
                     TRAEIDAVAGEIRAAGGKAHTVICDVTETQAMRRAVAAIPVLDVLVNNAGFNIPEPFV
                     EVSEEHLDQLLTLNVRAAFLVAQAAARKMLESPQRKARGGAILHMSSQMGHVGAPNRT
                     VYCLTKHALEGLTKAMALEFAAQGIRVLSIAPTFVETPLYRKMAARPEFAAWVQDRIP
                     MARVGQAEEVAAAVVYAASPGASLMTGTSLVVDGGWTAQ"
     gene            complement(20141..21433)
                     /gene="hisD"
                     /locus_tag="AC16O9_02480"
     CDS             complement(20141..21433)
                     /gene="hisD"
                     /locus_tag="AC16O9_02480"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6515513.1"
                     /translation="MATHLKRRIAQDVTDEIDRKVRHTVEEILADVKQRGDAAVREYS
                     EKFDKWTPKKLSKADIEAIMAKVAPGTISDIKFAQAQIRNFAEHQRAAIRDIEVETLP
                     GVKLGHKNIPVESVGCYVPGGRYPMVASAHMSIVTARTAGVKHIIACTPPNQGEPHAE
                     TIAAMVLGGADEIHVIGGVQAVAAMALGTESIRAVDMLCGPGNAYVAEAKRQLFGRVG
                     IDLFAGPTEILVVADDTADVEMIATDLLGQAEHGPTSPAILVTTSRKIADALPAEIER
                     QLKVLPTADVAGVAWRDYGEIILVDSLQEAVKEADRVAAEHVEILTKNPRYFLERMQN
                     YGALFLGPETNVSYGDKVIGTNHTLPTLGAARYTGGLWVGKFLKTCTYQEVTREASVK
                     VGEYCSRLCAIERFWGHKEQADLRLRRYGGQNVGLGAK"
     gene            21549..22394
                     /locus_tag="AC16O9_02485"
     CDS             21549..22394
                     /locus_tag="AC16O9_02485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6515514.1"
                     /translation="MSLKGRTLFITGGSRGIGLAIALRAARDGANVVIAAKTSAPHPK
                     LPGTIHTAVAEIEKAGGKGLAVQCDIREESQMLAAVEKTVAAFGGIDILVNNASAIQL
                     TGTLGCDMKRYDLMNGINARGTYLAGRCCIPHLKKSRNPHILTLSPPLDLSPKWFAPH
                     LAYTLAKYGMSLCTLAWAEEFRNDGIAANALWPRTTIATAAVQMLGGEAMMKQSRKPE
                     IMADAAHAILCKPSREFTGNFCIDDLVLHAAGVRDFSVYAAVPGTTRFLPDFFVPENT
                     PALPA"
     gene            complement(22453..23268)
                     /locus_tag="AC16O9_02490"
     CDS             complement(22453..23268)
                     /locus_tag="AC16O9_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6515515.1"
                     /translation="MSDFQNIRVRSEAGVAQVELHRPERVNALNKAMLLEINSALDAL
                     EADAGVRVIVLTGAGRGFSSGFDLKEQMERRPEGAKVWREVLDLDFSTTMRFWDSPKP
                     TIAAIHGACMAGAFEMALACDISVCSRDAVFGEPELKFGAGIVTMLLPWMVGPKAAKD
                     IILTGDDTVSAERALALGIVSRVVPDGEHLRTALRIARQMAVIDPDLVRDTKKALNRS
                     FEIQGMRAALDTALDIDHAVESHGSPDKRKFMDIAREQGLKAALAWREARFKV"
     gene            complement(23282..24748)
                     /locus_tag="AC16O9_02495"
     CDS             complement(23282..24748)
                     /locus_tag="AC16O9_02495"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6515516.1"
                     /translation="MSAAQPRLDALLVAAAERHAGREALAGERSLSYTAFLAEAESVA
                     EALQRSGLVRDEPLHVRVSNHPLDLVAFLGAWLAGGAVVPVHRTSPALAAAQIAERTL
                     ARFEWDALAATPLRQLAAVAPPPRPILEGGALIVFTSGSSGAPKGAVLSHRAFAGKLG
                     AIQSRLGFRADERTLLVLNITFSFGIWVALLTLLHGGRLRPVEKFAPATFLEQLSAES
                     ITRVALVPTMMRALILDHPAAALAKPNALRTVMIGGEALGQGLAESLRALFAPAGLID
                     IYGLTETSTSDFFLMPEDAERYAGCIGRPSPGVEFRIRGDDGAAVAQGAVGELCIRSP
                     FLMNGYLGQPELDPIDAEGWLATGDLARERDPGVVEIAGRKKELIVRGGNKIAPQEIE
                     FALSAHPKVAAALAVGRPDARLGQRIHALVLPRDASLTVEALRTFLRERLDRFKQPDV
                     FYFSRELPAGRTGKADRGRFAAMLEAGELAPAADVHPE"
     gene            complement(24754..26427)
                     /locus_tag="AC16O9_02500"
     CDS             complement(24754..26427)
                     /locus_tag="AC16O9_02500"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6515517.1"
                     /translation="MAVDLLVKGGLVIDGTGAPARSADVAIQDGRIAQIGTLRDGARR
                     TIDADGLVVAPGFIDPHTHYDAQICWDGALTPSSWHGVTSVVMGNCGVGIAPCRPAAR
                     EIAMRDLVNVEAIPFEVLERGITWDWESFPEYMQAAARRQPALNLGFLAPLTPFRHYV
                     MGEASMERAADAAQTAKIKALLGEAMDAGAFGFSSTLLNQHMGFAGRPLACRNASREE
                     LKAYCNALKERGKGAIEIAMTRQIGVMDDPELELLDFMLTESARPITFIAMFDRDDIS
                     EAVRTSLRKAAPMIARGARPQTSPLPLTREINMRNPFSFAGFPSWKRVFEDKSKAAQA
                     AVYRDPAFRAQFREELKRPAGFGNWERITVHEVKSPALKALEGRTVAEVARAQGKDGV
                     DAFLDLTLADDLENEFTMTSFNNRPERMAEILNNPAMLLGLGDGGAHLDMLCDSGYPT
                     YMLGTWVRERKVLTLEHAVQRMTSDPADFFGIKDRGRLAPGLAADLAIFDAATVGSLG
                     RPERRYDLPGGAKRMVMRSQGIEYTIVNGVVTWEGGKLTGAAAGAVLRS"
     gene            complement(26431..26736)
                     /locus_tag="AC16O9_02505"
     CDS             complement(26431..26736)
                     /locus_tag="AC16O9_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515518.1"
                     /translation="MSALFIVRAQVDPSVRDAFDRWYRDEHLPDALKAFKAKRAWRGW
                     SDTDASVHYAFYEFEDLARARAIPGSAEIKALIAEFDRMWGNKVTRARDVVDVVQAL"
     gene            complement(26733..27329)
                     /locus_tag="AC16O9_02510"
     CDS             complement(26733..27329)
                     /locus_tag="AC16O9_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515519.1"
                     /translation="MAEPQLVDPHDPVMTGENSFVRLSDDGGKTLAERVSHWRVLWSP
                     AGQGHALFIESTLTGKGPRVYADNAGVARYLQRLIEVLLHKPFADESLPVVDARFERT
                     GNSLSTVEERVIARNDEIVLSWWDLMPPFILTMPPGTLNRPLGVYSTFLPARSAQLSV
                     NGRAATARVFAQERSGRASSSCCLAWSETWTRPTGGKS"
     gene            complement(27339..27770)
                     /locus_tag="AC16O9_02515"
     CDS             complement(27339..27770)
                     /locus_tag="AC16O9_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6515520.1"
                     /translation="MAENLTGSCLCGAIRYSVSVPITELRVCHCTNCQKGSGTGGSVN
                     APLASSAFKLTQGTPKRHTGKADSGRTLHRYFCGDCGSPIYSQREEMPEMIALRVGTL
                     DNPGNMKITANIWTRSARPWSYIDPASKQFPGQPDAPAATK"
     gene            complement(27784..28518)
                     /locus_tag="AC16O9_02520"
     CDS             complement(27784..28518)
                     /locus_tag="AC16O9_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515521.1"
                     /translation="MPQPLASRTALVTGASRGIGRAIALALAAAGANVAVNYRERRAE
                     AEGAVNAIQALGRKAVALPADVSRADAVAALVRAAESALGPIDILVNNAGIAVRRGLD
                     ELTEEDFDRTLAVNLKSAFLCTQAVLAGMRERGWGRIVNISSGAARGAGLVGAHYNAS
                     KAGMEGLTRGYAARLAKEGVTVNAVAPSLIETEMIADHRKQAAARIPVGRLGTGEEVA
                     AVVVMVAANAYMTGQTVQVNGGLHFN"
     gene            complement(28553..28861)
                     /locus_tag="AC16O9_02525"
     CDS             complement(28553..28861)
                     /locus_tag="AC16O9_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515522.1"
                     /translation="MRELRVSDSSVTIEDSHVGGGTTGLYVSNSAVIATSVRFEGEIA
                     ITALASRLDLADVELVGSRAALTAPVRSHVVSSFGRVHSPNVRGNLHALFTFGPDKPL
                     "
     gene            28988..29848
                     /locus_tag="AC16O9_02530"
     CDS             28988..29848
                     /locus_tag="AC16O9_02530"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6515523.1"
                     /translation="MGLTFRKLHPLFAAEASAIDLRAVTDQPTLDAIRAAMDEYAVLV
                     FRDQRFDGGEQLAFAKRFDGTLHAKTGAAVLGRNRLGDEALTDISNVDESGQLMQAGD
                     RRRAYALGNRLWHTDASFEDPPGRYSMLLAKVVPPVAADTEFADLRAAYDALDDATRR
                     TLEGLRAHHSIAYSRMTLGFEFSAEEQRKLPGAVHPVVRANPRTGRRSLYLASHASRI
                     IDWPVPEGRLLLRELVERATQPQFVYRHEWREGDLVIWDNLATMHRGRPFDDTRHRRE
                     LRRVTTLDIA"
     gene            complement(29845..30393)
                     /locus_tag="AC16O9_02535"
     CDS             complement(29845..30393)
                     /locus_tag="AC16O9_02535"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR6515524.1"
                     /translation="MKLPQNSLFAMLLRARWWVSALVALGVFALTRLILPEGFALFAT
                     LPFIVIAVVVAWKEVRQPRGARLEAALERLRAMSWDEFAAGVEQGYRRVGYSVTRTEG
                     AADFELEKDSRVSLVSARRWKAARTGVEPLKDLVTAGERRGAVECVIVVTGELSEQAR
                     KLAGDKQIKLLEGAGLTALLKR"
     gene            complement(30390..32174)
                     /locus_tag="AC16O9_02540"
     CDS             complement(30390..32174)
                     /locus_tag="AC16O9_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR6515525.1"
                     /translation="MANPIEKWLDKLERCLARSDIDGTAKLFDDECYWRDLVAFTWTI
                     KTFEGRGEIRAMLSATLGAAAPGDFRALAAPVEHDDGVVEGWFAFETARGRGTGHLRL
                     RDGRCWTLLTTLQELKGFEEPAGPLRARGLEHGVVPGRLSWLERRRKDAAELGFALQP
                     FVLIVGGGQGGLALAARLKRLAVPTLVVEQNERAGDSWRKRYRSLCLHDPVWYDHMPY
                     LPFPDHWPVFSPKDKMGDWLEMYARVMELNCWTSTRCLKAAYDEAAAEWRVQVERSGR
                     VLELRPKHLVLATGMSGYPNLPDIPGADAFFGKIVHSSKFEGGEAWRGKCCVVLGANN
                     SAHDIAADLVEHGAQEVTMVQRSSTVVVTSDALMEHAWGKLYSEDALERGITTEIADL
                     TVASVPFRLLPSFQKPVYDAIRERDAELYAGLEKAGFQFDFGEDGSGIHATYLRRGAG
                     YYIDVGASQMVIDGRIRLKSPAHIERIAEQTVLLSDGSVLTADLVVLATGYGSMNQWA
                     AQLISPEVAARVGKCWGLGSDTKYDPGPWEGELRNMWKPTQQPGLWFMGGNLMQARHY
                     SLYLALQLKARFEGLDTPVYYVEPVHHP"
     gene            complement(32257..32562)
                     /locus_tag="AC16O9_02545"
     CDS             complement(32257..32562)
                     /locus_tag="AC16O9_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HIR6515526.1"
                     /translation="MKPPLSSYRERALKILPWICTRCAREFPASRLRELTVHHKDGNH
                     DHNPPDGSNWELLCIYCHENEHARVEDARAGGGGEKPQAPAMHKPLAALETLLKGKK"
     gene            32575..32997
                     /locus_tag="AC16O9_02550"
     CDS             32575..32997
                     /locus_tag="AC16O9_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR6515527.1"
                     /translation="MPTVQKSLNFRTRGRGTTDITAEVGQVVRDAGVRTGLCNVFLRH
                     TSASLILCENAAPAVRQDLETILARLAPDGDPAYAHDDEGPDDMAAHARSVLTASSLQ
                     IPVAEGRLALGTWQGIYLWEHRHAPHSRNVVVTVLGQS"
     gene            complement(32988..33413)
                     /locus_tag="AC16O9_02555"
     CDS             complement(32988..33413)
                     /locus_tag="AC16O9_02555"
                     /inference="COORDINATES: protein motif:HMM:NF023125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HIR6515528.1"
                     /translation="MPVGPDLIVAYLGAEYVVFGEPELVLHIGEASEALDELLDAQGA
                     GTAAYVTAANPRGRLAGATENVLATTALLEAQRDAGYACFAGEGRDPQGEWPAEPSVL
                     VVGIARAEAEVLGRSYEQNAIVFVERGKAPELVMLAAQD"
     gene            complement(33413..33832)
                     /locus_tag="AC16O9_02560"
     CDS             complement(33413..33832)
                     /locus_tag="AC16O9_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR6515529.1"
                     /translation="MAGVRVVLDTQVWLDWLVFDDPAVGRLREAQAAGRIEIVIDEVC
                     EAELVRVLGYDLGKFSLEPDEQTHCVARARGVSRRVPAGSPGELPRCKDPDDQKFLEL
                     AASTGARILVSKDQALLALARRVTRFRILAPQDCGEL"
     gene            complement(33820..34158)
                     /locus_tag="AC16O9_02565"
     CDS             complement(33820..34158)
                     /locus_tag="AC16O9_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515530.1"
                     /translation="MKKKRRARHHHHVYVVELDPAVLNLARFRRANPERDMLKPCVYV
                     GMTGLTPEARFAKHKAGVRANAYVQRFGLRLLPGLYAYANPMPYEAAREMEVELAIGL
                     REEGYAVWQG"
BASE COUNT         5573 a        12136 c        11455 g         5179 t
ORIGIN      
        1 cagctcggcg tgatgcgggg cgatttccgg atggcggtgc gcgtagtcct gctgcgcgtc
       61 ccacagcccc tgcagatcgc gcgccaggta cgcatgcagg ctgcgttcgg cgagcgcgtc
      121 gagcgcgtcg cgatgctcga gaaagtggcg cagcagcgcc acctgcgagt cgcgcgacat
      181 ctcgtcgaag gtgaacacct gctcctcgac gttctcgatt tgcgaaaccg gcaggcgccg
      241 ctcgcgcgcc agcgcgaaga gctgcgcatc gggcgccatt gcgcccgcgg gctgccgcga
      301 gatgcgcagg ttgagcagca cgccccaggg cttgagcttg ctgacgaact cgcgcggcag
      361 cccgatcggc tgcattgccg acacggcgcg ctcgaagtgc tcgtcgccga tatcctgcgg
      421 cagcgtgcgc ccgtccaggt acatcgcagc ctcgaggaag cgttcgcggc catacggatc
      481 ggcgaggtat tcgagcatca gcgagcgcgc ctgcccgaag gcgtcctgta ccgcagccgg
      541 cagtggttcc aggcgcgggt cgccggcgtg cagggtgccg aagacgtggc tcggctcgac
      601 gccggatctc accacgcgcc acagcaagcc gcgcgcgaag cgtcccgcgg gcgccgcccg
      661 caacggcagg gcgcacgcga gcgcagcggc aagaactgcg cgccgcctca tccagcgccg
      721 ggccgtgtca tggcctcggg ccgcacccag cgctcgaagt cctcggcgct gacatagccc
      781 agcgcgagcg ccgcctcctt gagcgtgctg ccctcgcggt gcgccttctt ggcgatctct
      841 gcggcccggt cgtagccgat gtgaggcgcg agcgccgtca ccagcatgag ggatcgctct
      901 accagctcgg cgatacgcgc cgcgttggcc tcgatgccgc gcgcacaatg ctcctcgaag
      961 gacgcgcagc cgtcggcgag caggcgcacg ctctgcagaa agttgtggat cagcatcggc
     1021 ttgtagacat tgagctcgaa ctgccccgag gcgccgccga tacccagcgc gacgtcgttg
     1081 gcgagcacct gcacgcagag catggtcagc gcctcggcct gcgtggggtt caccttgccg
     1141 ggcatgatcg agctgccggg ctcgttttcg ggcagcgaga tctcgcccag tcccgagcgc
     1201 ggccccgagg caagccagcg gatatcgttg gcgatcttgt gcagcgccgt ggcgagcgtc
     1261 ttgagcgcgc catgcgcgaa caccagcggc tcgtggccgg cgagcgccgc gaacttgttg
     1321 ggcgcggctt cgaacgtcat gcccgtggct tgcgacagat ccttcgccat gcgatcgccg
     1381 aactccgcat gcgtattgag cccagtgccg acggccgtgc ccccgatcgc cagctcgcac
     1441 agcccgggca gcgtcagctc gatggcggct tcggcctgcg cgagctgctc gacgtagccc
     1501 gagaactcct ggcccagcgt gagcggcgtg gcatcctgca agtgcgtacg gccgatcttg
     1561 acgatgccgg caaacgcctc ggccttggtg gcaagcgtcg cctccagccg ctcgagccgc
     1621 ggcagcaggt catgctcgac ggcgcgcgcg gcggcgatgt gcatcgcggt gggaaacgcg
     1681 tcgttggagg actgcccgag gttgacgtga tcgttgggat gcacgaggcg ccgcggcccg
     1741 cgctcgccgc ccagcagctc ggaggcgcgg ttggcgagca cctcgttcac gttcatgttg
     1801 ctctgcgttc ccgacccggt ctgccagacg accagcggga actcctcgtc gtgccggccg
     1861 gcgagcacct cgtcggcggc ccgctccacg gcgtcggcga tgttcggttc gagcagcccg
     1921 agcgcgcggt tgacgcgcgc cgccgagcgc ttcacctgcg ccagcgcgtg gatcacggcg
     1981 agcggcatgc gctcgcccga tatgcggaac aggcgccggc tgcgctcggt ctgcgcgccc
     2041 cagagccggt ccgcgggtac ctcgatcggg ccgaaggagt ccttctcctt acgcgtggcc
     2101 accataggcc ccgatcgtac taggacggcg cctgaccgtc ccggcggtcg cgctcggcga
     2161 tctcctggtg caccttcgcc atgtcgagcg ccttggcctg cgtgatcacc tggtcgagcg
     2221 ctgaggccgg cagcgcaccg gcctgctgga acagcaccac cttttcgcgg aacaccatca
     2281 gcgtcgggat ggagcggatg ttgaacgcgc ccgccagctc ctgctcctgg tcggtattga
     2341 tcttgccgaa caccacgtcg gcgtgacttt ccgccgcctg ctcgtagatc ggcgcgaagc
     2401 cgcggcacgg cccgcaccac ggcgcccaga agtccacgat cacaatgggg ctttcggtga
     2461 tcaccttctc gaagttgtcc ttggtcaatt cgaccgtcgc catgcgaatc cccgtcgaaa
     2521 aacgcgcagt gtactctctg ggcgtcatgg cggattacgt cggccgcttc gccccttcgc
     2581 ccacggggcc tctgcatttc ggctcgctcg tcgccgcgct ggcaagctac ctggaagcgc
     2641 gcgcggcgaa cggcaggtgg ttgttgcgca tggaggacct ggatcgggcg cgcgcgcaac
     2701 ccggcgctgc cgacgcaatc ctgcgcgcgc ttgagcgcct cggtttcgaa tgggacgggc
     2761 ccgtcctcat gcagagcgcg cgcctcgagc gctatcgcga ggtcctcgac gagctctcgc
     2821 ggcgcggcct cgcctacccg tgcagctgca cgcgccggga gctggaggat tccgcgcttg
     2881 ccatcgacgg tgcgcgcgtc tatcccggca cctgccggca cggcctcgcg cccggcaagg
     2941 ccgcgcgcgc gctacggttg cgcacgcacg gcgcgccgat cggcttcgcc gacgccatcc
     3001 agggctgggt cgagcagccc gtggagcagg cggtcgggga cttcgtgctg ttgcgcgccg
     3061 acggcgttat cgcctaccag ctcgccgtcg tggtggacga cctggaccag ggggtgacgc
     3121 acgtggttcg cggcgccgat ctgctggatt ccaccgcacg ccagatccat ctgcagcgcc
     3181 tgcttggcgc gtacacgccg tgctacgcgc acgtgccggt ggcgctgaac gcggcgggtg
     3241 agaagctttc caaacagacc gccgcgcgcc ctctcgacct ctccgatccg caagccgagc
     3301 tggcgcgcgc gcgccgcttc ctgggccaag gcgcatccgg ctgggatttg gcgcgcgtgc
     3361 cgcgcacgcg cgctatctcc gcgccggaat gacgaccgag gcaagcgatg ccagcaccag
     3421 cgcaagcgcg ctgaactcga gccagcccgg gcgctcgcca agaaacagca tgcccgagaa
     3481 cacgccgacc accggcgtca tcagcatgct cagcgagaag gtggtcaccg agaccgaggt
     3541 ggcgagcttg atccaggccc agtgcgccca ggcaaaggac agcagcacgt tgtaggccac
     3601 cgcgagcgcc ggccacaggc tgatatcctg cagcttgctg aagtcgtcga acagcatcgc
     3661 gccaacatag atcggcacgc caccgatcag catcatccac gccgtgagcg gcgctaccgg
     3721 tgcatgcacg ggaaacttct tctgcagcaa cgtgccgatc gcccaggtac aggccgcgcc
     3781 gagcaccagt agcgcgccaa gcggcgcgcc ccgcaggctg gtgaactcct ggaccagcag
     3841 cagcgccatg ccggtcatgc cgagggcgag cccggcgagc ttcctcgcgc tcatgcgctc
     3901 gcggaggaac cacaccgaga gcgggatcgc ccaggccggc atggtgtagg cgaggatcgc
     3961 tgcgcggccc gacggaatga ggcgcaggcc aaacaccacc agcacgttcc agcaggtgat
     4021 ggtgaaaaac gcgatcagcg ccaggcgtcg ccactggctg cgcgggatgg caaggggttg
     4081 accagtcgcc cgcagaaccg cgaacagcac accggagcca gccgcgagac agaacgtgcg
     4141 gaacgtgaac ggcgcgactt ccgagatcgc caccttcatc gccgtccaat tgaagcccca
     4201 gaccagcgtg atgcccagga gcacccacca caggtgccgc ggcaggtgct cgggggccgg
     4261 cgccggactc atgcccggct cacgagccat acggcgagca tggcgagcgc catgccggcc
     4321 gcggcaaacg ccgtgtaggt ctcgccgaac aggggccagg ccacgagtgc ggtgcagggc
     4381 ggcacgaggt acatgaggct cgcgaccttg gtggcggcac cgtggcggat gagccagaac
     4441 agcaggagca cggcaccgag cgacagcgca aataccagcc acaccagggc gaccaccagc
     4501 tccgcggacc agaccacagg acgcgattca aacagcagcg cgtagggggt gagcaccagc
     4561 agcgcggcgc cgaactggat cgcggcgccg gtgcgcaggt cgacgctcgc gcagtggcgc
     4621 ttctgccaca ccgcgcccag gctgatgctc accagcgcca gaaccgacag cgcggtcgcc
     4681 gccaagccca cgccctcgag cacccacttc tcgcgcacca ccagagcgac cccggcgatg
     4741 cccagcgcga ggccgagcca ctggcgggcg ttggtgcgct cgccgagcag ccagcccgaa
     4801 agcaccgcgg cgaggatcgg ctgcagcgcg gtaatcaggg cggaggcgcc cgccggcatg
     4861 ccttggtgca aagagaggaa gacgccaccc agatagccgc cctggatcag ggccccggca
     4921 aacgccatgt gcatgaatcg aactcccgag gtcggccagg gcgcgcgcat cgccacgcac
     4981 agaggcagta gcacgaccag caccagcacg aaccggatca cgagaaatgt cgccggctcc
     5041 acgtaaggaa gtcctaactt cgccccgatg aagcccgtgc tccagagcac cacgaagagc
     5101 cccgggaaca cccacgcgaa acgctggacc gggtccgggg ctgggctcat ggacggattg
     5161 tccacggtgc cagatcggca cgatgagccg tttcgccccc ttcccaggga agccgtttga
     5221 aacgcctaga atgccgctcc actgaaattc tgcagtgcct caatggctta tgttgcaccg
     5281 caaaattcat ttgactttaa aaggggaatc tttatactgg cgattgttgc gctgcaccaa
     5341 atcgcgccga cccaaagctt aaaccgattt ctcataggag caccaaaatg tacgtgaccc
     5401 ccgagcagat ccaagccacc ggcaaggcca atgtggaaac catgctgtcc ctcgccgcga
     5461 cgcaattcgc cgcgatcgag aagctcgcca ccctgaacgc caacgccgtc aagaccgcgt
     5521 tcgaagacag cctcagcaac acgcgcgcgc tgtttggcgc taaggacgtg caggagttcg
     5581 tgacgctgca gaacacgctg gcgacccccg cgatcgagaa ggccatcgcc tattcgaaaa
     5641 gcgtgtacga agtcgccacc gagaccaatg ccgagctgtc gaagatggca gagcagcgcg
     5701 tcgccgagtg gaacgagaac ttcagctcga tgctcgacaa ggcggtaaag aacgcccccg
     5761 ccggctccga cgtcgcggtc gccgcggtca agtcgatgat cgccgccgcc aactccgcct
     5821 acgacaacat gaacaaggtg gccaagcagg cgaccgagat cgcggaagcc aacgtcaacg
     5881 ccgcgtccga gaccgtgaaa ggcctgacca aggcaaaaaa gaccgcctga gcgccaaacc
     5941 gggccgcacc ccggcccgat ctagcgccat gcgtaagccc aagaagaaga agaagaagaa
     6001 gaagtaagca aaagcccgcc gaccggcggg ctttttcatt gcagcgcggc ctgcaccgcg
     6061 tcttccacct tgtcgagaaa gacaaagcgc agcttcttcc tcgcctcggc cggcacgtcc
     6121 tccaggtctt tctcgttcct gcgcggcagc atcaccgtgg acaggcccgc gcgcagcgcc
     6181 gccagcgtct tctccttgac gccgccgatc ggcagcacca gcccgcgcag cgagatctct
     6241 ccggtcatgg caacgtccga ccgcaccggc ttaccctgca ggagcgaggt gagcgccacg
     6301 aacatggcca cgcccgcgct cgggccgtcc ttgggcgtgg cgcccgccgg cacgtgcaca
     6361 tggatatcgg tcttctccag cgcgtcgccg ctccaggtct tggcgagcgt cagcgccgcc
     6421 tgcgcggatt ccttcattac gtcgccgagc tgacccgtga ggatgagctt gcccgacccg
     6481 ggtaccttgg aggcctcgat gaacaggatg tcgccgccca cgggcgtcca ggcgaggccc
     6541 gtcgccacgc ccggcaagga cgtgcgcagc gccacctcgt tctcgaactt gcgcgcaccg
     6601 aggatcggct gcaggtcggc cgcgccgacc gtcacgcact gcgcgttgcc ctccgcgatg
     6661 cacatggcga cgtggcgcag cacggcgccg atctcgcgct cgaggttgcg cacgcccgcc
     6721 tcccgcgtgt agctggcgat gatctcgcgg atcgctcctt cggtgagtgc tgcctgctct
     6781 gctttcagcc cattcgcctc cagctgcttc ttcaccaggt accggcgcgc gatctgcagc
     6841 ttttcttcct ccgtgtagcc cggcagctgg atcacctcca tgcggtcgcg caacggcccg
     6901 ggaatcgtgt cgagcacgtt cgccgtggcg atgaacatga cccgcgacag atcgaagtcg
     6961 acgccgaggt agttgtcacg gaacttggcg ttctgctccg gatcgagcac ctcgagcagc
     7021 gccgaggacg ggtcgccgtg gaaaccgccc atgcccagtt tgtcgatttc gtccatcatc
     7081 agcacgccgc cgcgcgactc ggcgcgcttc agcgcctgga taacgtttcc cggcagcgcc
     7141 ccgatgtagg tgcggcgatg gccgcgaatc tccgcctcgt cgtgcaggcc gccgagcgcc
     7201 acacgctgga acttgcggcc cgtcgccctg gcgatcgact ggccgagcga ggtcttgccc
     7261 acgcctggcg ggccgacgaa gcacaggatc gggctcctgc cctccgggtt gagcttgcgc
     7321 acggcgagat actcgagaat gcggcgcttc accttctcca gcccgtggtg atcctcgtcg
     7381 agcacgcggc gcgcctcggc gatgtccacc gccagttgcg gctcggcctt ccagggcagc
     7441 tcggccatca gctcgagcca ggtgcgcagc atggaggctt cgcccgagcc ctcgcccatg
     7501 cgcgacaagc gcttgaactc ttttttcgcg tgccggagcg tttcttccgg cattccggcg
     7561 gcctcgaggg acttctgcag ttcctcgagc tccgcggccg tatcctcgcc ctcgccgagc
     7621 tccttctgga tctggcgcat ctgctcgcgc agcacgtgct cgcgctggcg ctcgtcgaat
     7681 tccttcttcg tcttgtcgcc gatctccttc gacaggcgca gtacgcttac gcgctcggcg
     7741 agatgcgcaa gcaccaggtc caggcgcggc ttcagctcga aggcctcgag caccgcctgc
     7801 ttctcttcgt tctcgatgtc gagaaggttc gccaccatgt cggccagctg cggcgcagag
     7861 tcgatcgcct gcaccgcgcc ggccagctcg tcgggcacgt tcggcagcag cttgatcgcc
     7921 tcgacggcct gcgccttcag ctgcaggaag cgcgcctcga tctcttcgcc gccttcggtc
     7981 gcctcgggta cccagaccac gcgtcccacc aggaacggcc agccttcgag gaactcggtg
     8041 acgcgaaagc gcgtctgccc ctgcacggcc aggtggtgaa cgccttcggc ggcgggcacg
     8101 tagcgcacga tttcgccggc cgtgcccacc cagtgcaggt cgtcggcgcc gacctggttc
     8161 gttttcgggt cgcgctgcag cagcaaaccc agcttgcggc cctgccgcgc cgcctcctgc
     8221 acggcggcga cggacgagcc gcgccccacc gcgagcggcg taatgatgcc agggaacagc
     8281 accgcgcttc gcagcggaac gaggatcagc gcgtcctccg gaatgggccg cacggccagc
     8341 gcggtgcccc ggcccgcgcg gccgcgcggc gcctcggcgc ccgtgccgcc gccgccctcg
     8401 agcgtttcct gtcgtttcct gcgccagaat tccatggctt ctcctgccga ttggcgcacg
     8461 gtcgtgcgca ccgatagcct aggtgcggtc gaatcgggcg gaatcaagcc gcccctgccc
     8521 cggatttcct ggggaaattg agtttcgcgc tagccggcga ggaccttagg cagccacagc
     8581 acgagcccgg gaaaggcgac cagcagcgcc acgcgcacga tctcggcgcc gaagaacggc
     8641 atcacgccca ggaacgtccg gctcatcgga acgtcgcgcg cgagcgagtt gatcacgaat
     8701 acgttcatgc ccaccggcgg agtgatcagt ccgagttcca ccacgatgag cgccaggata
     8761 ccgaaccaga tcttgagctc ctccacgctc atgccgaagt ccaggcccga gatcaccggc
     8821 cagaagaacg ggatcgccag caggatcatc gacagactgt ccatcaaaca gccgagtgcg
     8881 ataagcgcca ccagcagcag caccagcacc tcaagcggcg ccagtcccga tgtcgccatc
     8941 acttcggcgg cggcctgcgg cacgccgcca cgcgccatga agatattgag cagttgggca
     9001 ccaagcagga tgaggtagat catcccagcc gtctttgcag tttcgagaag cgccgctttc
     9061 atcccctgca aattcacgga tctgcgcgcg aaagccagca ccgtgaccag aaagacgccg
     9121 accgcggccg ccggcgtggg attgaacagg ccgaagtaga tcccgccgat cacggcgccg
     9181 aagatcgcca gcaccggacc cacgccgatg gtcgcggcaa tgaactcctc gcgcgtaacg
     9241 cgatcgcccg gcggcccggc atcgggccgc acgcgcacgt agatcgcgat cgtaagcagg
     9301 aacatcgcta tggcgatcag gcccgggatg aaggccgcaa tgaacatatc gacgatgctc
     9361 gcctcgacga cgatcgcgta gaccacgagc accacagagg gcgggatcag gatgccgagc
     9421 acgccaccag cggcaagggt gccggtggcg agttccgggc tgtagcggta gcggcgcagc
     9481 tcgggcaggg cgatctgtcc catggtggag gccgtagcga gcgaagagcc gcagaccgcc
     9541 ccgaaaccac cgcaggctgc gatcgcggac atcgccacgc cgccgcgcat ccagccgagc
     9601 caggcgttgg ccgcgcggaa caggtcccgc gaaagccccg cgcgcgaggc gatgttgccc
     9661 atcagcacga acatcggcag caccgacagg tcgtagatcg agaactgcga atagaccagc
     9721 gtcttcagct gcgacagcag gatctggggc ccgctcagta tcgcgatccc gatcgcgccg
     9781 accaccagca tggcgtgcgc gatcggcacg cggatcgcga tcagcgcgat cagcgaggcg
     9841 aggccgacga gcccggtgag aaaatgggga tccaagcggg ctcgctcaac cgcgcagcgc
     9901 gcgcacttcg tccttcagca cgatcgcgca ggcgagcacc agcagagcca gcgaggcgag
     9961 cgccggcacg tacgcccacc agatcggtat cttgaggatt gcggtggtct ccacgtactt
    10021 tcggtagtcg agcaggccgg cccaggtacg ccacgcgagc accagcgcga ccacgagcgc
    10081 caacagcccg ccgaacaggc ggaacgcggc gagcgcacgc ggcccggcac gcgcagtaaa
    10141 caggtccgcg gtgacgttgg cgtccgtctg ctggcaatac ggcagaaacg cgaacaccgc
    10201 gaccgcgacc aggatttcgg tgagctcgaa gtccccgggg agcggcttgc cgaagaccca
    10261 cccggagaac gcgctccagg tggtcatcag ggcgaccgcc agcaggacga cgccgcctgc
    10321 cagcgcccac cattcgatgg cgcggcgggc gaggcccgac ggccccgccc gtgcccgcgc
    10381 cggaccttca tccatgcgct acttggagtg cttagagatc cgcgcgcgcg cctggtctac
    10441 cagcgccttg ccgtcgatgc ccttcgaatc gacctcggcg atccagcgat ccacgacggg
    10501 ctcgagcttc gccttgaaca tcgcgagctc ggcctccgtg agctggatat gcttgttgcc
    10561 cgccttcttg gcaacggcaa tccccccggc ttcgctgtcg gtccagacct tgccgactgt
    10621 gcgccaccgg gcttcgccgt tgttggcttc gaaggccttc tgcacgtcgc gcggaagctt
    10681 ggcccaggtg ttcttgttca tcgacacctg gaaggtcgtg gtgccgaagc gcatccggtt
    10741 cgggccttcg acctggacct tggtgacgtc ctggagcttg agcggcggga tgatctccca
    10801 cggaatgaac gcgccgtcga cgaccttctt cgacagcgcc tgcggcagcg ccggcacggg
    10861 catcgaggcg ggcgcggctc ccagcgcctc gatgatccag gcgccggtgc gcgtcgggat
    10921 gcgcattttc aggcccttca ggtcgtcggg cgtgcgcact tccttctccg ccatgtgaat
    10981 cgcctggccg gcgtgcacgt gcaggaacat gaccttgacg ctctcgtagt ccggcgcgat
    11041 ggcgccgttc aggaacatct cgcgcatcgc caagttcgtc gcggtggcgt tgttcgtgtg
    11101 cacgaacggc agctcgaaca cctccgagcg cgggaacagg cccgccgtgt agccgttcac
    11161 cgtccatacg atgtccacca cgccgtctcg cacctgattg atcagctcgg gcggcttgcc
    11221 gccgagcgcc atcgacggga agagctcgat cttgaccttg ccgcccgagg ctttctccac
    11281 gtccgccgcc cacggcgcga gcatcttcgt gtgcgccggc gccttgggcg gcaagaagtg
    11341 atgcagtttc aggttgattg tctgcgcgga ggccggcgcg gacagtgccg cgcaaactgc
    11401 ggcgacggca agcgaaatag tgcagaaacg cggtttcatc tgtgataccc ctcctctcgt
    11461 aggatccgat cgaaacgacc atcgcccgtc gcggcgcggt tctgggccgg cctgccgagg
    11521 gctgcatggc atatgatgcc agacgccgcg cggcccgttt tgactatcca cgtgcccggg
    11581 ttagcatcgg cgcacctccc ggaaggaact atgttgcagg aacgcctgcg cgcgctgacg
    11641 cgcgacacgc tgcgcggcat gcgccgcggc atcgaaaagg agtcgctgcg cacgcggccc
    11701 gacggcgcgc tggcgctcac gccgcatgcc gccgcgctcg gctcggcgct cgcgcacccg
    11761 cacatcacca ccgacttcag cgagtcgcag ctcgagctca tcaccggcac ccacctgacc
    11821 gtggagggct gccttgacga gctgacgcgc atccaccagt acgtggtgcg cgtcatcggc
    11881 gaggagctgc tgtgggccgg cagcatgccc tgcggcttgc ccgcggagca cgagattccg
    11941 atcggccgct acggtcgctc gaacatcggc cgcgccaaga cggtctaccg caatggcttg
    12001 tcgtggcgct atgggcggcg catgcagacc atctccggca tccattacaa ctggtcgctg
    12061 cccgggctgg gcaacgccga gtactttgcg ttgatccgca atttccggcg ccacggctgg
    12121 ttgctgctgt acctgttcgg cgcctcgccc gccgcgtgcg cgagcttcgt cgaaggccgc
    12181 ccgcacccgc tgcgcgagct gcgtccgggg acgctgtacc tgccgcacgc gacgtcgctg
    12241 cgcatggggc ggctcggcta ccagagcgat gcgcaatcgt cgctcatggt cgtgtgcaac
    12301 gacctggagt cgtatggcgc cacgctgcag cgcggcctga cgcaggtata cccggcgtac
    12361 gagcgcatcg gcatccgcga gccgggcgcg cagggcgacg atccggacag ttaccggcaa
    12421 ctctccacca cgttgctgca gatcgagaac gagttttacg gcaagatccg ccccaagcgc
    12481 ccgatccagc agggcgagcg gccgctgcac gcgctgcgcg agcgcggcgt cgagtacgtc
    12541 gaagtgcgtt tgatggacct tgacccgtac gcggccgtag gcatcaccgc gcccgtgatg
    12601 cgctttctcg acgtgttcct gctgcactgc ctgctctcgg agagcccgcc ggacaccgtg
    12661 gacgaagtcg cgtgcgtggg ccgcaatcag gagtgcgtgg cggcgcgcgg gcgtgagccg
    12721 ggcctgcgca tcggctgcgg accggaggaa atcggcctgc agaaatgggg cgagcagctg
    12781 ctcaccgaat gcgcccccgt ggccgaggcg ctggacgcgg cgctcggcgg gcagctgtac
    12841 cgggaagcgc acgccgcgca ggccgccgcc atccgcgacc cggcgctgac gccgtcggca
    12901 cgcatactcg cggacatgca ccggtacggc gactcgtacc gtgacttcgc gctcgcgcaa
    12961 tcgcgccggc accgcgaagt gctcctcgcg ctgccattcc cggccgccga ggaagagcgt
    13021 ttccggcacc tcgcggcggc gtcgatcgcg gagcagaagg cgatcgaggc cgccgatagg
    13081 ctgccgttcg aggagtaccg aaagaattac ctggccgtcg agcggctggg cgtctgatgc
    13141 aaaggaggag atgacatgcc gaccaccact gccagcgggc tgatctacga ggagaaagtc
    13201 gtcggcacgg gcgacaccgc gcaatcgggg caggacgttt ccgtgcacta caccggctgg
    13261 ctgctctacg gcggcgagcg cggcaagaaa ttcgactcca gcaaggatcg cggcgaggcg
    13321 tttggctttg cgctcggcgc cggccaggtg atcaagggct gggacgaggg cgtgcagggc
    13381 atgaaggtcg gcggcacgcg cgtgctgacc atcccccccg cgctcggcta cggcgcgcgc
    13441 ggtgcgggtg gcgtgattcc gccaaatgcg acactggtat tcgaggtcga gctgctgggg
    13501 atcgagaaat agttccgcaa ggcgggaccc gggcccgcct tacaatgcgg tccgtagggg
    13561 gcaactctca gctgttggaa ggagtgccct atggcggatt ctccggtctg gatgatctgg
    13621 cctggcaacg cagcgctatc ggcgctggtg tggttcgtga tcgcgatgcc gttcctctat
    13681 gcggcacgcg ctcccgtgca tggtctgttg cgcgcgctgg gcctgctggc cggcgggccg
    13741 ttgcgccttg cggcgcgcgc cctgctcgcc tccgcgcagg agctgcgcga gcgcaaccgc
    13801 gccgtgctct tcgcgcaggg gcgtgaggag gcgggcgagc gcatcgcgcg cgagttcgaa
    13861 cgcgttgctg cgctcgtcaa gcgcgacctc gaaggctacc ccgcactgca gcgcaagctc
    13921 ctcgacgaga ttactcgcgt cgaggaagac taccgcaagt gcggtgaagt accgccgccg
    13981 cccccggact gggtggaggc agtcgccgcg gtggcgaaga tcaagccgaa cggcaacgag
    14041 ctggtgcagc gcctgctcga ggagatcaac gtctccatca aggcgatcca cgacaagacg
    14101 atcgcggagt accgccgcgc ctaccaggcg cgccaccgta tcctcgacgg cttcatgccg
    14161 ttctggcgct cgctcgacaa gacgctcgcg cgcgccgacc gccgcatgac ggacctagcc
    14221 gagcgcgcca cggccatcga cgggcagatg gagcgctacg aggccatcgc caagaaagcc
    14281 gacaaggcgg agcacgcgct gacggtctcg gccgcgacgc agttcttcat cgccacgctg
    14341 gtgcttgcca tcgccgcggg cggcgcgctc atcaacttca agctgatcgc gctgccgatg
    14401 tcggaaatgg tgggcgccgg cgactacctg acggcgtcgc tgcgcacctc cgaggtggcc
    14461 gccctggtga tcatcttcgt cgaggcgacg atggggctgt tcctgatgga gacactgcgc
    14521 atcacgcacc tgttcccgcg catccacaac ctgggcgaga ccatgcgccg gcgcatgatg
    14581 tgggcgtcct tcaccctgct gctgatcctc gccggcatcg aggcggcgct cgcgctgatg
    14641 cgcgacatgc tgatcgccga caagcaggcg ctgctgcagt cgctcgccgc aacgcaggcc
    14701 gtggcgtccg acggctggct ctcgggcatc cccaccgccg ggcagatgct gctcggcttc
    14761 atcctgccgt tcgcgctcgc cttcgtcgcc atcccgctcg agtcgttcat caactcggca
    14821 cgcaccgtcg gcggcgcggc gttggtcgtc cttgcgcgct cggcggcggt cgtgctgcgc
    14881 gtggcgggca atttcctgcg ccagttgtgc aaggtgctga tcacccttta cgacatcacc
    14941 atcgtcgtgc cgctgcttgc cgagcgctgg gtgaagggca tgcggccgcc ggggcttggc
    15001 cagggcgcgc gcggcgacct gaaggccggg gggcaatcat gaagcgccgc gcgttcctgg
    15061 tctcgctgcc gctactcggc gccgcgcagc tcctcggctg caccgacccg cgcagccacg
    15121 cgcacgccgt ctacctcctg gtcgacacct cgggcaccta cgccgaggag gtcggcaagg
    15181 cgcagctgat cgtcaactac ctgctcggcg cgctgcagcc gggcgattcg ctcgccgtgg
    15241 cgcgcgtgcg cagccgcagc ttcagcgaaa aggacatcat cgccaaggcg accttcgacc
    15301 agcgcccctc gcaggccaac gcgcaaaagc gcctgttcaa ggaccgggtg gaggcgttca
    15361 cgagcacggc gcgcggcagc gtgcataccg acatcaccgg cggcctgatc cagggcgcgc
    15421 tgttcctgaa cgagaccggc gccggcaaga agacgatcct catcttctcg gacatgcagg
    15481 aagagctcga ccgcgccacg gtgcgcgatt tcccgatcga cctcaaggac atccgcgtcg
    15541 tggcgctgaa cgtggtgaag ctgaagaccg acaacctcga cccgcgccgc tacctcggcc
    15601 ggctggagga ctggcagaag cgcgtgaagt cggccggcgc cagcgagtgg cgggtgatca
    15661 acgatctcga acacctcgac gcgatgttga aaagctgacc cgcagggttc ttgttgccta
    15721 acgccagcga cgcgggcgga acctccccgg caggtacagc ggatgcgcgg gctcgccatt
    15781 ggcgttgagc cgcaggacat gcagcgcgac acccgcgccg cgcaggagcc gctgcaccgc
    15841 ctctgagcgg ccgaggtata gcccgtggtt gccccaggcg caaacgacga gctgcgccgc
    15901 cttagccgcc cgcaggatcg cggcatcgtt acccctgccc acgggatccg gcgcggcgcg
    15961 catctcgacg gggtcggtcg cccgccacgc gaatacgttc gtcaccagca acgcgccgta
    16021 gccccagcgc tcggcatagt cgcgcgcgcg tgtgcaggtc ggatcgagca ccgtttcgtc
    16081 ggcagtactt ggattgagca tgaggaagtt gacgaccggg cgtgcgtcgt cccagcagcg
    16141 ccacagcagc gtgcgccagc gccggcaggc ggaaaacctc gccccggacg cgttcattcg
    16201 gccgcgcgca cgcgcagcac gcgcgcccac caggccgccg cccccgcggc gagcccgcag
    16261 ccggcggcaa tggcgaaagc gagcatgtag ctgccggtgc gatcgaagag gagcccggcc
    16321 atccagggac cgagcgcgct gccaacggcg gcgcccatca ggccgacgcc gacgatggtg
    16381 ccgaaatgcg gtccgccgaa acggtcgctc accatcgccg gcgtgagcga cgccgtggcc
    16441 gagtaaccca cgcccagcag cacggcataa ccgtagatcg cccaggccgc gggcgcgccg
    16501 gcgagcgcga ccagcgccgc gaccgcagcc agcatgatcg cgatgccggc aacgtagacc
    16561 acttcgcgct ccatgcggtc cgagagccag ccgccgcccg tcttgccgac gatgctggag
    16621 acgccgatca cgccgaccac cgaggccgca accagcgctg acaccccctg gtcgacaagg
    16681 taggcgacct ggtgcacgtg caacgtctgc gagcaaaggt tgccgaagaa gaacgccgcg
    16741 agcatcagcc agaacggcgc gccgcgcatc gcttgtgcaa gggtaatcac cggcagcggc
    16801 ttggccgtgg ccgcggcggg ctccggcacc ggccgccgca ccggcgagcg caggtgcagg
    16861 tacagcgacg acggcacgat ccacagcacg cagatcgcgc caaggacgcg aaaggcggtg
    16921 cgccagccgt aggcgtcgat cagcaactgc gcgagcggca ccaccagcag catgccgacg
    16981 ccgacgcccg agctgacgat gccgagcgcg aggccgagcc tgtcctggaa gtcgcgctgc
    17041 acctgcacca gcgcgggcac ccagcccgcg gccgcgacgg cgatcgcagt aaacacgccg
    17101 aagctgaggt agagatgcca gggtgcggcg atggcgctgt tcgcccagag cgcgaggccg
    17161 agggcgaccc cgcccgccgc catcaggcgc atggggcgca agcggtcgca cagcgccccg
    17221 acgaccggat tgaccgcacc gtggaccagc gtgaacaccg agaaagcgcc ggcgagcacc
    17281 gagcgactcc agccgaactc cttcaacagt gccaccagca cgacgctgta ggagtaccag
    17341 acgccatagg caagcgcgag cgtaagggcg atgacgccca ggctcagggc acggcgcgac
    17401 ggcggcaagg cggttctagc ggatgctcag gcggcactct cgaccaggcg catgtcgcgt
    17461 tcggcggcgt tgccgagcgc gtcgagcgcc ttcttgtacg ccggaccctc gtaggcggcg
    17521 cgcgctttct ccacgctgtc gaactcgacg accaccgtgc gctggttcat gcccagctcg
    17581 tacgtcttgg tcggcatgcc gcgcaccagg aagcggccgc cctcggcctg gaccgccggg
    17641 ccggcgagct tcgcgtaggc ctcaagcgcc ttcggatcct tgagcgagcg gtaggtgaca
    17701 accatatagg ccttggccat cgcgatctcc tccggcagta agttgaggcc gccattttca
    17761 cacggcgctg cgctgccgcc gcgcagtttc gccgcgcgcc ggcatcgttg ctatagtcgg
    17821 cgcattccga cccggaagga gacacgcgtg agcaccatcc ccaatcacgc cctacgccag
    17881 ctgcgcgcgg ggaaactcgc catcggcctc ggcctgcgcc aggcgcgcac cgtcgacgtc
    17941 ggccagatcc tgaagactgc cggcttcgac tggatgttca tcgactgcga gcacaactcg
    18001 atgggcaccg acactgccgc gcagatcgcc tccgccgcgc ttgccatcgg catcaccccc
    18061 gtggtgcgcg tcgcagggaa ggaacactgg ctcgcctcgc gcatgctcga caacggcgcg
    18121 cagggcgtcg tcgtgccgca cgtcgacacc gagcaggaag ccaagcgcat cgccgaccac
    18181 tgccgctttc cgcccatcgg ccaccgctcg atgggcggcg gcctgcagca gctcggcttc
    18241 gcctcgatgc cggtgggcga ggcggcgaac atcgtgaacg acgagacgct ggtcgtggtc
    18301 atgctcgaat cgccgaaagg catagagaac tgcgaggcca tcgccgcggt gaagggcatc
    18361 gacgcgctgc tcatcggcac caacgacctg tgcttcgaga tgggcatccc gggccagttc
    18421 gacgatccgc gggtggccga ggcctacaag aaggtgatcg ccgcgtgcaa gaagcacggc
    18481 aagttcgcgg gcatgggggg catgtacacg aaagagctgc tcgagcgcca catcgccatg
    18541 ggcgtgcagc tgatcctgtc gggttccgac ttctcgctgc tgcagcaggc ggccaaggcg
    18601 cgcgctgacc tggtgcgcgg cttcgagggc aaggcatgac cccgcacatc gcgacggtcg
    18661 tcgtcagcgg cagcccgatg gaggtgttcc tgtttgcgcc cgcgggggcg gggccgcacc
    18721 ccgggctcgt cctctgccag cacattcccg tcgggcacac cggcgtagag aacgatgtgg
    18781 tgacgctgaa gaccgccgag cgctactgcg agaacggcta tttcgtcgcc gtgcccttca
    18841 ttttccactg gtggccgaag agcgccgaca tcgagctcaa gcgcgcggag ttccgcgacg
    18901 actggacgcg actcgacctc gacgccgcat tcgagctgct ggcgacgcag ccgggcgtcg
    18961 acaagagccg cgtcggcatc gtcggtcact gctggggcgg gcgtgtcgcc tggctcggcg
    19021 cgtgcaccaa tccgcgctac aaggccgccg cgatcttcta cggtgggcgc gtgaagctcg
    19081 tcatgggcaa gggcaatccg ccggcgatcg atctcacgcc cggcatgcgc tgcccggtga
    19141 tcggcttctt cggcaacgag gacaccaatc ccacgccggc ggatgtcgac gactacgagg
    19201 ccgcgctcgc caaggcgggc gtcgagcacg tgttccaccg ctacgacggc gccggccacg
    19261 ccttccagtc gttcaacaac cccgagcgct accgcaagga agcaagcgag gatgcctgga
    19321 acaaggtgct ggcgttcttt ggcgggaaac tcggcaggac gtagccgatg ctactgcgcg
    19381 gtccagccac cgtcgacgac caggctggtg cccgtcatca gcgaagcgcc cggcgaggct
    19441 gcgtacacga cggccgccgc cacctcctcc gcctggccga cccgcgccat cgggatgcgg
    19501 tcctgcaccc acgcggcgaa ctcggggcgc gccgccatct tccggtaaag cggcgtctcg
    19561 acgaacgtcg gggcgatgga aagcacgcga ataccctgcg cggcgaactc caacgccatc
    19621 gccttggtga ggccctccag ggcatgcttg gtgagacagt aaaccgtgcg gttgggtgcg
    19681 ccgacatggc ccatttgcga tgacatgtgc aggatggcgc cgccgcgcgc cttgcgctgg
    19741 ggcgattcga gcatcttgcg cgccgcagcc tgcgcgacga ggaaggccgc gcgcacgttg
    19801 agcgtgagca gctggtccag gtgctcctcg ctcacctcga cgaacggctc cgggatgttg
    19861 aagccggcgt tgttgacaag cacatccagc acaggaatcg ccgccaccgc cctgcgcatg
    19921 gcctgagtct cggtgacgtc gcagatcacc gtgtgcgctt tgccgcccgc ggcgcggatt
    19981 tcgccggcga ctgcatcgat ctctgcgcgc gtgcgcgcca tgagccagac ctccgcgccc
    20041 gcctgcgaga gcgccagggc gcaggatcgg ccgatgccgc gacccgcgcc cgtgaccagc
    20101 gcaacgcggc catcgagccg gaaccgcgac gcgatatcgc tcacttggcg cccagaccga
    20161 cgttctgacc gccgtagcgg cgcaggcgca ggtcggcctg ctccttgtgc ccccagaagc
    20221 gctcgatggc gcaaaggcgc gagcagtatt cgccgacctt gacgctcgcc tcgcgcgtca
    20281 cctcctggta ggtgcaggtc ttcaggaact tgccgaccca aaggccgccg gtatagcgcg
    20341 ccgcgccaag cgtgggcagc gtgtggttgg tgccgatcac cttgtcgccg taggagacgt
    20401 tggtctcggg cccgagaaac agcgcgccgt agttttgcat gcgctcgagg aagtagcgcg
    20461 gattcttcgt caagatctcg acgtgttccg cagccacgcg gtcggcctcc ttgaccgcct
    20521 cctgcagcga atccacgagg atgatttcgc cgtagtcgcg ccaggcaact ccggcgacgt
    20581 cggccgtggg caggaccttg agctggcgct cgatctcggc gggcagcgca tcggcgatct
    20641 tccgagacgt ggtgacgagg attgccgggc tggtcggccc gtgctccgcc tggccgagaa
    20701 ggtcggtggc gatcatctcg acgtctgccg tgtcgtcggc caccacgagg atctccgtcg
    20761 gaccggcgaa gaggtcgatg cccaccctgc cgaagagctg gcgcttggct tccgcgacgt
    20821 acgcattgcc cggtccgcag agcatgtcca ccgcgcgaat ggactcggtg ccgagcgcca
    20881 tggccgcgac ggcctgaacc ccgccgatga cgtggatctc gtcggcgccg ccgagcacca
    20941 tcgcggcgat ggtctcggca tgcggctcgc cctggttggg aggcgtgcag gcgatgatgt
    21001 gcttcacgcc tgccgtgcgc gccgtgacga tgctcatgtg agcggaagca accatcgggt
    21061 aacggccacc gggcacgtag cagccgacgg actctaccgg gatattcttg tgcccgagct
    21121 tcacgccggg cagggtctcg acttcgatat cgcggattgc ggcacgctgg tgctcggcga
    21181 agttgcggat ctgcgcctgc gcaaacttga tgtcgctgat ggtgccgggc gcgactttcg
    21241 ccatgatggc ctcgatgtcc gccttggaaa gcttcttcgg cgtccacttg tcgaacttct
    21301 ccgaatactc ccgcaccgcg gcatcgccgc gctgtttcac gtcggcaagg atttcttcga
    21361 cggtgtggcg cactttccgg tcgatttcgt cggtgacgtc ctgggcgatg cgacgcttga
    21421 gatgggtggc catgggttcc tcgaagtccg gagtcagagc ggaattctaa ggtggcggcg
    21481 cgttcgcggg gatgtatagt ccttcgagct gcttcgcgtc catctgcggc atcacgccag
    21541 gagacctcat gagcctgaag ggaagaaccc tgttcatcac cggcggcagt cgcggcatcg
    21601 ggctggccat cgcgctgcgt gcggcgcgcg acggcgccaa cgtcgtgatc gccgccaaga
    21661 ccagcgcgcc gcatccgaag ctgccgggca ccatccacac ggcggtggcg gagatcgaga
    21721 aggccggggg caagggcctg gccgtgcagt gcgacatccg cgaggagtcc cagatgctgg
    21781 ccgcagtcga gaagacggtt gcggccttcg gcggcatcga catcctggtg aacaacgcca
    21841 gcgcgatcca gcttaccggc acgctcggct gcgacatgaa acgctacgac ctgatgaacg
    21901 gcatcaacgc gcgcggcacc tacctggcgg ggcgctgctg catcccgcac ctgaagaagt
    21961 cccgcaaccc gcacattctc accctgagcc cgccgctcga cctgtcgccg aagtggttcg
    22021 cgccgcacct tgcctacacc ctcgccaagt acggcatgag cctgtgcacc ctcgcctggg
    22081 ccgaggagtt caggaacgac ggcatcgccg ccaacgcgct ctggccgcgc accacgatcg
    22141 ccaccgccgc ggtgcagatg ctcggcggcg aagcgatgat gaagcagtcg cgcaagccgg
    22201 agatcatggc cgacgcggcg catgcgatcc tgtgcaagcc gagccgcgag ttcaccggca
    22261 acttctgcat cgacgatctg gtgctgcacg cggcgggcgt acgcgacttc tccgtgtacg
    22321 cggcggtccc ggggaccacc cggttcctgc cggatttctt cgtgccggag aatacgccgg
    22381 cgctgcccgc ctagcatcgc gccgcagtcc gccggagcgg cgacggcgct cgcgccgtga
    22441 agcggtgaac gtctagacct tgaagcgcgc ttcgcgccag gcgagcgccg ccttcaggcc
    22501 ctgctcgcgc gcgatgtcca tgaacttgcg cttgtcgggc gagccgtgcg actcgacggc
    22561 atggtcgatg tcgagcgcgg tatcgagcgc cgcgcgcatg ccctggatct cgaagctgcg
    22621 gttgagcgcc ttcttggtgt cgcgcaccag gtccggatcg atcaccgcca tctggcgggc
    22681 gatgcggagc gcggtcctca ggtgctcgcc gtcgggaacc acgcggctca cgatgccgag
    22741 cgcgagcgcg cgctccgcgc tcaccgtgtc gtcgcccgtc aggatgatgt ccttggcggc
    22801 cttcggcccc accatccagg gcaggagcat ggtgacgata ccagcgccga acttcagctc
    22861 cggctcgccg aacaccgcgt cgcgcgagca caccgaaatg tcgcatgcga gcgccatctc
    22921 gaaggcgccg gccatgcagg cgccgtggat cgcggcgatc gtcggcttgg gcgagtccca
    22981 gaagcgcatc gtggtggaga aatccaggtc cagcacctcg cgccacacct tcgcgccttc
    23041 ggggcgccgc tccatctgct ccttcaggtc gaatcccgat gagaagccac gccccgcgcc
    23101 tgtcagcacg atcacccgaa ccccggcgtc ggcctcgagc gcgtccagcg ccgagttgat
    23161 ctccaggagc atcgccttgt tgagcgcgtt gacccgctcg gggcggtgca gctcgacttg
    23221 cgccacgccc gcctcgctcc tgacacggat gttttggaaa tcgctcatgc catgctcctc
    23281 cctattccgg gtgcacgtcc gcggccggcg caagctcgcc cgcctccagc atcgccgcaa
    23341 agcgcccgcg atcggccttg ccggtgcgcc ccgcgggcag ctcgcgcgag aagtagaaca
    23401 catcgggctg cttgaaccgg tccagccgct cgcgcaagaa ggtgcgcagg gcctccaccg
    23461 tgaggctcgc gtcgcgcggc agcaccagcg cgtggatgcg ctggccgagg cgtgcgtcgg
    23521 gccggcccac ggcgagcgcc gccgccacct tcggatgcgc gctcagcgcg aactcgatct
    23581 cctgcggcgc gatcttgttg cccccgcgca cgatcagctc cttcttgcgc ccggcgattt
    23641 ccaccacgcc ggggtcgcgc tcgcgcgcga ggtcccccgt ggcgagccag ccctcggcgt
    23701 cgatcgggtc gagctcgggc tgcccgaggt agccgttcat caggaacggg ctccggatgc
    23761 acagctcgcc caccgcgcct tgcgccacgg cggcgccatc gtccccgcga atgcgaaatt
    23821 cgacccctgg cgaaggccga ccgatgcagc cggcgtagcg ctcggcgtcc tccggcatca
    23881 ggaagaagtc gctggtcgag gtctcggtca atccgtagat atcgatcagc ccggccggcg
    23941 cgaacagcgc gcgtagcgat tcggcaagcc cctggccgag cgcttcgccg ccgatcatca
    24001 ccgtgcgcag ggcgttcggc ttggcaagcg ccgccgccgg atggtcgagg atcagtgcgc
    24061 gcatcatggt cggcaccaag gcgacgcgcg tgatcgactc ggcgctcagc tgctcgagaa
    24121 aggtggccgg cgcgaacttc tccaccggcc gcagccggcc gccgtgcagc aacgtgagca
    24181 gcgccaccca gatgccgaag ctgaaggtga tgttgagcac cagcagcgtg cgctcatccg
    24241 cgcgaaagcc gaggcgcgac tggatcgcgc cgagcttgcc ggcgaacgcg cggtgcgaca
    24301 gcaccgcgcc cttcggcgcg cccgacgagc ccgacgtgaa cacgatgagc gcgccgccct
    24361 cgagaatcgg tcgcggcggc ggcgccacgg ctgcgagctg ccggagcggc gtcgcggcca
    24421 gcgcgtccca ctcgaagcgc gccagcgtgc gctcggcgat ctgcgcggcg gccagcgcgg
    24481 gcgaagtgcg gtgcaccggc accaccgcgc cgcccgccag ccaggcgccg agaaaggcga
    24541 cgagatccag cggatggttc gacacgcgaa cgtgcagggg ctcgtcgcgc acgaggcccg
    24601 accgctgaag cgcctcggcg accgattcgg cttcggcgag gaacgccgtg tagctgagcg
    24661 accgctcccc tgcgagcgcc tcgcgcccgg cgtggcgctc cgcggcggcg acaagcagcg
    24721 catccaggcg cggctgcgcg gcgctcatcc gcgtcaggag cgcagcacgg cacccgctgc
    24781 tgcgccggtg agcttgcccc cctcccaggt caccacgccg ttcacgatcg tgtactcgat
    24841 gccctgcgag cgcatcacca tgcgcttcgc gccgccggga aggtcgtagc gccgctccgg
    24901 ccgcccgagc gagccgaccg tggccgcatc gaagatcgcc aggtccgcgg ccagcccggg
    24961 cgcgaggcgc ccgcgatcct tgatgccgaa gaaatccgcc gggtcggacg tcatgcgctg
    25021 caccgcatgc tccagcgtca gcaccttgcg ctcgcgcacc caggtgccga gcatgtaggt
    25081 cgggtagccg gagtcgcaca gcatgtcgag atgcgcgccg ccgtcgccca gcccgaggag
    25141 catcgcgggg ttgttgagga tctcggccat gcgctcgggc cggttgttga acgaggtcat
    25201 ggtgaactcg ttctccagat cgtcggcgag ggtcaggtcg aggaacgcgt cgaccccgtc
    25261 cttgccctgt gcccttgcca cctcggccac cgtccgccct tccagggcct tgagcgccgg
    25321 ggacttcacc tcgtgcaccg tgatgcgctc ccagttgccg aagcccgcgg ggcgcttcaa
    25381 ctcctcgcgg aactgcgcgc gaaacgccgg gtcgcggtac accgccgcct gcgccgcctt
    25441 cgacttgtcc tcgaacacgc gcttccacga cgggaagccg gcgaacgaga acgggttgcg
    25501 catgttgatc tcccgcgtca gcggcagcgg cgaggtctgc ggccgcgcgc cacgcgcgat
    25561 catcggcgcc gccttcctca aggaggtgcg cactgcctcc gagatgtcgt cgcggtcgaa
    25621 catggcgatg aacgtgatcg ggcgcgcgct ttcggtgagc atgaagtcga ggagctccag
    25681 ctcggggtcg tccatcacgc cgatctgcct ggtcatcgcg atctcgatcg cgcccttgcc
    25741 gcgctccttc agcgcattgc agtaggcctt gagctcctcg cggctcgcgt tgcggcaggc
    25801 tagcggccgg ccggcgaagc ccatgtgctg gttgagcagc gtgctggaaa agccgaacgc
    25861 gccggcgtcc atcgcctcgc cgagcagcgc cttgatcttc gcggtctgcg ccgcgtcggc
    25921 ggcgcgctcc atcgacgcct cgcccatcac gtagtgccgg aacggcgtca gcggcgcgag
    25981 aaagccgagg ttgagcgcgg gctgcctgcg cgccgcggcc tgcatgtact cgggaaagct
    26041 ctcccagtcc caggtgatgc cccgctcgag cacctcgaac gggatcgcct ccacgttcac
    26101 caggtcgcgc atcgcgatct cgcgcgccgc gggacggcag ggcgcgatgc cgacgccgca
    26161 gttgcccatc accaccgagg tcacgccgtg ccacgacgaa ggcgtgagcg cgccatccca
    26221 gcagatctgc gcgtcgtagt gcgtgtgcgg atcgatgaag ccgggcgcca ccaccaggcc
    26281 gtcggcgtca atggtgcgcc gtgcgccgtc ccggagcgtc ccgatctgcg cgatacgtcc
    26341 gtcctggatc gcgacgtcgg cgctgcgcgc cggcgcgccg gtgccgtcga tgaccagccc
    26401 gcccttgacg aggagatcga cggccacggc ttagagcgcc tgtaccacgt cgacgacgtc
    26461 gcgcgcgcgc gtcaccttgt tcccccacat acggtcgaac tccgcgatca gcgccttgat
    26521 ttccgccgag cccggaatgg cgcgggcacg cgccaagtcc tcgaactcgt agaacgcgta
    26581 gtgcaccgag gcgtccgtgt cgctccagcc gcgccaggcg cgtttcgcct tgaacgcctt
    26641 cagcgcgtcc ggcagatgct cgtcccggta ccagcggtcg aaagcgtccc ggacggaagg
    26701 atcgacctga gcgcgcacga tgaagagggc gctcatgatt tcccccctgt gggtcgcgtc
    26761 caagtctccg accaggcgag gcagcagctc gacgatgccc ggccggagcg ctcctgcgca
    26821 aacaccctgg ccgtcgcagc cctgccattt accgagagct gcgcgctgcg cgcggggaga
    26881 aacgtgctgt agacgcccaa gggacggttc agggtccccg gcggcatggt gaggatgaag
    26941 ggcggcatca ggtcccacca gctgaggacg atctcgtcgt tgcgcgcgat gacgcgctcc
    27001 tcgacggtgc tgagcgagtt gccggtgcgc tcgaagcggg cgtcgacgac cggcagcgat
    27061 tcgtcggcga agggcttgtg cagcagcacc tcgatcaggc gctgcaggta gcgcgcgacg
    27121 cccgcgttgt cggcgtagac gcgcgggccc ttgccggtga gcgtgctctc gatgaacagg
    27181 gcgtgtccct gtccggcggg agaccagagc acgcgccagt ggctcacgcg ctcggcgagc
    27241 gtcttgccgc cgtcgtccga caggcggacg aaggaatttt ccccggtcat caccgggtcg
    27301 tgcggatcga ccagctgcgg ctcggccatg ctccccccct atttcgtcgc cgcgggcgcg
    27361 tcgggctgcc cggggaactg cttgctcgcc ggatcgatgt acgaccaggg gcgcgcgctg
    27421 cgcgtccaga tgttggcggt gatcttcatg tttcccgggt tgtccagcgt ccccacgcgc
    27481 agcgcgatca tctcgggcat ctcctcgcgc tggctgtaga tcggcgagcc gcagtcgccg
    27541 cagaagtagc gatgcagcgt gcgaccgctg tcggccttgc cggtgtggcg cttgggcgtg
    27601 ccttgcgtga gcttgaaggc gctcgaggcg agcggcgcat tgacgctgcc gccggtgccc
    27661 gagcccttct ggcagttggt gcaatgacac acgcgcagct cggtgatcgg cacgctcacg
    27721 ctgtagcgga tcgcgccgca caggcagctg ccagtgagat tctcggccat cggtgtcccc
    27781 ctctcagttg aagtgcaggc cgccgttcac ctgcacggtc tgcccggtca tatacgcgtt
    27841 cgcggccacc atcaccacca ctgcggcgac ctcctcgccc gtgcccaggc gccccacggg
    27901 aatccgcgcc gcggcctgct tgcggtgatc cgcgatcatc tccgtttcga tcagcgaagg
    27961 cgcaaccgca ttcaccgtga cgccctcctt cgccagccgc gccgcatagc cgcgcgtgag
    28021 gccctccatc ccggccttcg acgcattgta gtgcgcgccg accagtccgg cgccccgcgc
    28081 cgcgcccgag gagatgttga cgatgcgacc ccagccgcgc tcgcgcatgc cggcgagcac
    28141 cgcctgcgtg cacaggaacg ccgacttgag gttgacggcg agggtgcggt cgaaatcctc
    28201 ctcggtgagc tcgtccaggc cgcggcgcac cgcgatgccc gcgttgttga cgagaatgtc
    28261 gatgggccca agcgcggatt ccgcggctcg cacgagcgcc gctaccgcgt cggccctcga
    28321 aacatcggcc gggagcgcaa ccgctttgcg gccaagggcc tggatggcgt tgaccgcgcc
    28381 ttcggcctcg gccctgcgct cgcggtaatt gacggcgacg ttcgctccgg ccgcggccag
    28441 cgccagcgcg atcgcgcggc cgatgccgcg cgatgcgcca gtgacgaggg cggtgcggga
    28501 ggcaagaggc tggggcatcg ggatttcccg gcaattatgc ggcctggcgc cgctacagcg
    28561 gcttatccgg accgaaggta aacaacgcat gcagattgcc ccgcacgttg gggctgtgca
    28621 cgcggccaaa tgaggacaca acgtggctgc gcacgggcgc cgtgagcgcg gcgcggctgc
    28681 cgacaagctc cacgtcggca agatcgaggc ggctggcgag cgccgtgatc gcgatctcgc
    28741 cttcgaagcg cacgctggtg gcgatcacgg cggaattgct gacgtacagg cccgtcgtgc
    28801 cgccgccgac gtggctgtcc tcgatcgtga cgctggagtc cgacacgcgc agctcgcgca
    28861 cgcgggcacc gcggccagcg cccgggcgca gcccaggaaa acggcaagcg cggccgtggc
    28921 gagccgggtc aggttcggtt ccatcctggc tggcagtcta aaggcgtgcg tggcatagaa
    28981 tcgcccgatg gggctgacct tccggaagct gcacccgctg ttcgccgccg aggcgagcgc
    29041 gatcgacctg cgcgccgtca cggatcagcc gaccctcgac gccatccgcg cggcgatgga
    29101 cgagtacgcg gtgctggtgt tccgcgacca gcgcttcgac ggcggcgagc agctggcgtt
    29161 cgccaagcgc tttgacggca cgctgcacgc gaagaccggg gccgcggtcc tgggcaggaa
    29221 ccgcctgggc gacgaagccc tcaccgacat ctccaacgtc gatgaaagcg gacagctcat
    29281 gcaggccggc gaccggcgcc gtgcctacgc gctcggcaac cgcctgtggc acacggacgc
    29341 gtcgttcgag gatccgccgg gccgctactc gatgctgctc gccaaggtcg tgccgcccgt
    29401 cgcggccgat accgagttcg ccgacctgcg cgcggcctac gacgcgctcg atgacgccac
    29461 ccggcgcacc ctggagggac tgcgcgccca ccattccatc gcctactcgc gcatgacgct
    29521 cggcttcgag ttctcggccg aggagcagcg caagctcccc ggcgccgtgc acccggtcgt
    29581 gcgcgccaat ccgcgcaccg gccggcgctc gctctacctc gcctcgcacg cttcgcgcat
    29641 catcgactgg ccggtgcccg aggggcggct gctgctgcgc gagctcgtgg agcgcgcgac
    29701 gcagccgcag ttcgtctacc gccacgaatg gcgcgagggc gacctcgtga tctgggacaa
    29761 cctggcgacc atgcaccgcg gccggccgtt cgacgacacc cggcaccggc gcgagctgcg
    29821 ccgcgtcacc acgctcgata tcgcctagcg cttcagcagc gcggtcaggc ccgcgccttc
    29881 cagcagcttg atttgcttgt cgccggcgag cttgcgcgcc tgctcgctca actcgcccgt
    29941 caccacgatc acgcactcca cggccccgcg ccgctcgccc gctgtgacca ggtccttcag
    30001 cggctcgacg ccggtgcgcg cggccttcca gcgccgcgcg ctcacgagcg aaacgcggct
    30061 gtccttctcc agctcgaagt ccgccgcgcc ctccgtgcgc gtcaccgagt agcccacgcg
    30121 gcggtaaccc tgctccacgc cggcggcgaa ctcatcccag ctcatcgcgc gcagtctctc
    30181 cagcgcggcc tcgagccgcg cgccgcgcgg ctggcggacc tccttccagg cgacgaccac
    30241 ggcgatgacg atgaagggca gcgtggcgaa aagcgcgaac ccctcgggga ggatgagccg
    30301 cgtcagagcg aacacgccca gcgcgaccag cgcgctcacc caccagcgcg cgcgcagcag
    30361 catcgcgaac agcgagttct ggggcagttt cacggatgat gcaccggctc cacgtaatac
    30421 acgggcgtgt cgagcccctc gaagcgcgct ttcagctgca gcgcgagata gagcgaatag
    30481 tgccgcgcct gcatcaggtt gccgcccatg aaccagagcc ccggctgctg cgtcggcttc
    30541 cacatgttgc gcagctcgcc ctcccacggg cccgggtcgt atttcgtgtc ggagcccagg
    30601 ccccagcact tgccgacgcg cgcggccacc tccggcgaga tcagttgcgc ggcccactgg
    30661 ttcatcgagc cgtagccggt ggcgagcacg acgaggtcag cggtcagcac cgagccgtcc
    30721 gagagcagca ccgtctgctc ggcaatgcgc tcgatgtgcg cggggctctt cagccggatg
    30781 cggccgtcga tcaccatctg cgaggcgcca acgtcgatgt agtagcccgc gccgcggcgc
    30841 aggtacgtcg cgtgaatgcc cgagccgtcc tcaccgaagt cgaactggaa gcccgccttt
    30901 tccaggccgg cgtagagctc ggcgtcgcgc tcgcggatcg cgtcgtagac cggcttctgg
    30961 aacgacggca gcagccggaa cggcaccgac gcgaccgtca ggtcggcgat ctcggtggtg
    31021 atgccgcgct ccagcgcgtc ctccgagtag agcttgcccc aggcgtgctc catgagcgca
    31081 tcggaggtga cgaccacggt ggaggagcgc tgcaccatgg tgacctcctg cgcgccgtgc
    31141 tcgaccaggt cggcggcgat gtcgtgcgcc gagttgttgg cgccgagcac cacgcagcac
    31201 ttgccgcgcc aggcttcgcc gccctcgaac ttgctcgagt gcacgatctt tccgaagaac
    31261 gcgtcggcac cgggaatgtc ggggaggttg ggatagccgg acatgccggt ggcgagcacc
    31321 aggtgcttcg gccgcagctc gagcacgcgg ccgctgcgct ccacctgcac ccgccattcg
    31381 gcggccgcct cgtcgtaggc ggccttcagg cagcgcgtcg aggtccagca gttgagctcc
    31441 atgacccgcg cgtacatctc cagccagtcg cccatcttgt ccttgggcga gaacaccggc
    31501 cagtggtcgg gaaacggcag gtagggcatg tggtcgtacc acaccgggtc gtgcaggcac
    31561 agcgaccggt aacgcttgcg ccacgagtcg cccgcacgct cgttctgctc gacgacgagc
    31621 gtcggcaccg ccaggcgctt caggcgcgcg gcgagcgcga gcccgccctg gccgccgccg
    31681 acgatgagca cgaacggctg cagcgcgaag ccgagctcgg ccgcgtcctt gcgccggcgc
    31741 tcgagccagg acagccgtcc gggcaccacg ccgtgctcca ggccccgcgc gcgcaacggt
    31801 cccgccggct cttcgaaccc cttcagctcc tgcagggtgg tgagcagggt ccagcaccgg
    31861 ccatcgcgca gccgcaggtg cccggtgccg cgccctcgtg ccgtttcgaa cgcaaaccac
    31921 ccttcgacga cgccgtcgtc gtgctctacc ggcgcggcga gcgcccggaa atcgcccggc
    31981 gccgccgcgc cgagcgtcgc cgagagcatg gcgcggatct cgccgcgccc ctcgaaggtc
    32041 ttgatcgtcc acgtgaacgc gaccaagtcg cgccagtagc actcgtcgtc gaacagcttc
    32101 gcagtgccgt cgatgtcgct gcgcgcgagg caccgctcga gcttgtccag ccacttttcg
    32161 atgggattgg ccatgggggg cgggcgcatt ttcgcccaca tcgcgcatcc gctctgcgcc
    32221 ggatgtcctg tggacggaaa acggcgggaa tgagaactac ttcttgcctt tcagcagggt
    32281 ttcaagtgcc gcgagcggct tgtgcatcgc gggagcctgc ggcttctcgc cgccgccgcc
    32341 cgcgcgcgca tcctcgacgc gggcgtgctc gttttcgtgg caatagatgc acagcagctc
    32401 ccagttcgag ccgtctggcg ggttgtggtc gtgattgccg tccttgtggt gcaccgtcag
    32461 ctcgcgcagg cgcgaggccg gaaactcgcg cgcgcagcgc gtgcagatcc agggcaggat
    32521 cttgagcgcc cgctcgcggt acgaggacaa tggcggtttc acgcagcgct tatgatgccc
    32581 actgtgcaga aatcactcaa cttccgcacg cgcggccgcg gcaccaccga tatcacggcg
    32641 gaggtgggcc aggtggtgcg cgatgcgggc gtgcgtacgg gcctgtgcaa cgtgttcctg
    32701 cggcacacca gcgcctcgct catcctgtgc gagaacgccg cgcccgcggt gcgccaggac
    32761 ctggagacga tcctcgcgcg gctggcgccg gacggcgacc ccgcctatgc gcacgacgac
    32821 gagggtcccg acgacatggc ggcgcatgcg cgatcggtgc tgacggcgag ctcgctgcag
    32881 attccggtgg ccgaaggccg gctcgccctg ggcacctggc aggggatcta cctctgggag
    32941 catcgccacg cgccgcactc gcgcaacgtg gtcgtcaccg tgctcggtca gtcctgagcg
    33001 gcgagcatca ccagctcggg cgccttgccg cgctcgacga acacgatcgc gttctgctcg
    33061 tagctgcggc cgagcacctc ggcctcggcg cgcgcgatgc ccaccaccag cacgctcggc
    33121 tcagcgggcc attcgccctg cgggtcgcgg ccttcgcccg cgaagcaggc gtagcccgca
    33181 tcgcgttgcg cctccagcag cgcggtggtc gccagcacgt tctcggtcgc gcccgcgagg
    33241 cggccgcgcg gattggccgc ggtgacatag gccgcggtgc ccgcgccctg ggcgtcgagc
    33301 agctcgtcga gcgcctcgct tgcttccccg atgtgcagca ccagctccgg ctcgccgaac
    33361 accacgtact ccgcaccgag gtaggcaacg atcaggtcgg gaccgaccgg catcacagtt
    33421 ccccgcagtc ctgcggcgcc aggatgcgaa agcgcgtcac gcgccttgcc agcgcgagca
    33481 acgcctggtc cttggagacc aggatccgcg cccccgtgct cgccgccagc tcgaggaact
    33541 tctgatcgtc gggatccttg caccgcggca gctcgcccgg gctgcccgcg ggcacgcgcc
    33601 gcgacacgcc gcgcgcgcgc gcgacgcagt gcgtctgctc gtcgggctcg agggagaact
    33661 tgccgagatc gtagccgagc acgcgcacca gctccgcctc gcaaacctcg tcgatcacga
    33721 tctcgatgcg ccccgcggcc tgcgcttcgc gcagccggcc gaccgcaggg tcatcgaaca
    33781 ccagccagtc gagccagacc tgggtatcga ggacgaccct cacccctgcc atacggcgta
    33841 gccctcctcg cgcaggccta tcgccagctc cacctccatc tcgcgcgccg cctcgtaggg
    33901 catcgggttg gcgtaggcgt agagccccgg cagcagccgc aggccgaagc gctgcacata
    33961 ggcgttcgcc cgcacccccg ccttgtgctt ggcaaagcgc gcctcggggg tgagcccggt
    34021 catgccgacg taaacgcagg gcttgagcat gtcgcgctcc gggttcgccc tgcgaaaccg
    34081 cgcaaggttc agcaccgcgg ggtcgagctc gaccacgtag acatggtggt gatggcgggc
    34141 gcggcgcttc tttttcatgc ctgcccgcgc aagcttacac tgcgtcccgt attcccctgc
    34201 cgcccgagta ccagcgccag atcacgcgcg aggagatgaa cgcgctgccg atccgccgct
    34261 acgagggcga ggtgtgcctg gtcgcctcgg ccgccgacct cgagcgcgcg atcgccgatg
    34321 tcggcgcgga acaggtgctc ggc
//
LOCUS       DBNHKE010000043         7073 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1116626, whole genome shotgun sequence.
ACCESSION   DBNHKE010000043 DBNHKE010000000
VERSION     DBNHKE010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1116626"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..2583
                     /locus_tag="AC16O9_02570"
     CDS             229..2583
                     /locus_tag="AC16O9_02570"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6515531.1"
                     /translation="MNDSTVAADTRDPVCGMPVTPGKEAGRLTREGKSYLFCSKHCLH
                     TFSADPARYAQAPASAHGGHAHHAHAAPKAAAAAAPAGAQYTCPMHPEVLKDAPGDCP
                     ICGMALVPVGAAAANDDNAELRDHTRRMWTGVVLSIPMVILAMSPMVGIAEPFGLAPR
                     ARGWIEFVLGTPVVLWVGWPLLAKFWRSVRGWRLNMYSLIGLGVGLAYLFSLVAILFP
                     EIFPKEFREHDGSVGTYFEAAGVIVTLVMVGEVLQMRAMGQTSQAIRQLLELAPNLAW
                     RLKEDGTEEQVPLDSVHEGDRLRVKPGDKVPVDGTVLEGASRIDESMITGEPVPVAKK
                     SGDRVTGATVNGSGSLVMRAERVGSETLLSRIVHMVAEAQRTKAPIQRLADLIAAYFV
                     QTVVVIAVLTALVWWFFGPEPALTYALLNSVAVLIIACPCAVGLATPISMTVAMGRGA
                     GMGILFRNSEAIEHMRKIDTVVVDKTGTLTLGKPALTDFACEGIEEAEALALVAGVEQ
                     LSEHPIATAIVQGAKSRKVAPARAEGFDAVNGMGVEAHINGRKVLVGSRAFLQQRGAE
                     PGAWEERAEKWRGEAKTVVFFAVDGRTYGIAAVADPVKDSTPEAIAALKQRGVRIVML
                     TGDSKRTAAVVARQLGIDEAVGEVLPENKALEVKRLQSEGRKVAMAGDGINDAPALAQ
                     ADVGIAMGTGTDVAMESAGVTLVKGDLRGIARAAALSRATMRNIRQNLVFAFGYNALG
                     IPIAAGVLYPAFGLLLSPIIAGAAMAASSLSVITNALRLRRTSF"
     gene            2593..2994
                     /locus_tag="AC16O9_02575"
     CDS             2593..2994
                     /locus_tag="AC16O9_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR6515532.1"
                     /translation="MPTTATAKYVFGKTVGLGFDEAIARVTEALAKEGFGVLTEIDVK
                     ATLKKKLGIDVAPYRILGACNPQYAHKAIEFEPQIGALLPCNVVVREEKGKTRIDIMD
                     PQAVLQLVERPEIEGIATEVRQRLERVLASL"
     gene            3075..3284
                     /locus_tag="AC16O9_02580"
     CDS             3075..3284
                     /locus_tag="AC16O9_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6515533.1"
                     /translation="MIELSVQGMTCGHCVSAVTRAVKTVDPEADVQVDLGTGKVRVDG
                     RSSAEALGKAIADAGYPAAEAAAPK"
     gene            complement(3315..3737)
                     /gene="cueR"
                     /locus_tag="AC16O9_02585"
     CDS             complement(3315..3737)
                     /gene="cueR"
                     /locus_tag="AC16O9_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HIR6515534.1"
                     /translation="MNIGQAAKHSGVSAKMIRHYEGIGLIPKAARTFSGYRTYSGNDV
                     HTLRFIRQARNLGFSIKQIEHLLGLWRNQRRPSSKVKALALEHIAALDARVAELQAMK
                     RTLQALAEHCHGDERPECPILDGLAAPDKPAPDRAHRH"
     gene            complement(3752..5980)
                     /locus_tag="AC16O9_02590"
     CDS             complement(3752..5980)
                     /locus_tag="AC16O9_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6515535.1"
                     /translation="MDISATSAAPAASADLDSDAYDLAIRGMTCASCVGRVERALNKV
                     PGVRSAQVNLATERAHVVAAAGLGIGALREAIAAAGYEADPLDLRQSAPPKQTAHALP
                     DWWRVAVAAVLTLPLVVPMIGLAVGAPWTLSGWLQLALSTPVLFWLGAPFYVAGWKAL
                     RAFSGNMDLLVAIGTSAAYGLSVFHLLTGGGAHELYFEIAAALITFILLGKWLEARAK
                     RQTTAAIRALEALRPERALVRRNGKDVEVPVGSIAVGDLVVVRPGERIAVDGEIREGR
                     SHVDESLITGESLPVAKGAGERVTGGAINAEGLLVVATRAVGAESTLARIIRMVENAQ
                     AAKAPIQRLVDRVSAVFVPIVLLIALATLLAWGFATGNWTSAVLYAVAVLVIACPCAL
                     GLATPTAIMAGTGVAARHGILIKDAEALEIAHSITTVAFDKTGTLTEGRPQLLAFEPL
                     AGPRDELLRLAAALQQGSQHPLARAVTERAGADGVSVPVASDSKALPGRGVEAVVEGR
                     RLYLGSSRLMQELGVDTAALSGRERALAAQGRSISWLAEETDGARRLLGLLAFGDAVK
                     AGAREAIAQLHAQGVKTVMLSGDNLGSATAAARALGIDEVRAEVLPADKAQTVAALRG
                     KRRVVAMVGDGINDAPALAAADVGIAMATGTDVAMHTAGITLMRGDPRLVADAIGVSR
                     RTYRKIRQNLFWAFFYNVVGIPLAALGFLNPIIAGAAMAFSSVSVVTNALTLRNWKPG
                     AK"
     gene            6170..>7073
                     /locus_tag="AC16O9_02595"
     CDS             6170..>7073
                     /locus_tag="AC16O9_02595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR6515536.1"
                     /translation="MKEFLHRAPHSRRRFLAGLAGLSALPLLPARGSAQAMPALVEAR
                     LVAAPREVQLVPEKYPATGVWAYNGSVPGPVLRARQGERLRVHVENALAEQTTVHWHG
                     IRLPNAMDGVPFITQRPIEPGGGFTYEFTLPDAGTFFYHPHQRSYEQVGRGLSGALIV
                     EERTPPEVDRDVLWVLGDYRLSADASIRGGFGHFMDLSHAGRIGNTVTLNGHMPESFR
                     VRAGERLRLRLINAAPARVFALEFRGHRPWVIALDGQPVEPHEPPGGAVVLGPAMRAD
                     LILDMTAAPGSRHAVHDGFYARRPY"
BASE COUNT         1085 a         2456 c         2481 g         1051 t
ORIGIN      
        1 catgcacctg ttccacggtc acggcgggca cggggggcat ggcgggcacc acggcgccaa
       61 gcaggacaag ggcgacaagg cgcccggagc gtgaacggcg tcgagtccta cgggttgtgg
      121 ccgctggcgg tcatcgattc gctcgtgccc atcagcttcg ccgcttgacg ttcccaccgt
      181 gggaaggtgg agactgagcg caacctaatc cagggagcaa ggtgacccat gaatgactcg
      241 accgttgcgg ccgacacccg ggatcccgtg tgcgggatgc cggtcacgcc cggcaaggaa
      301 gccggccggc tgacccgcga aggcaagagc tatctgttct gctcgaagca ctgcctgcac
      361 acgttcagcg cggatccggc gcgctatgcg caggccccgg catcggcgca cggcggccac
      421 gcgcaccacg cgcacgccgc accgaaggcg gcggcagcgg ctgcgccggc aggcgcacag
      481 tacacctgcc cgatgcatcc ggaggtgctc aaggacgcgc ccggcgactg cccgatctgc
      541 ggcatggcgc tcgtgccggt gggcgctgcg gcggccaacg acgacaacgc ggagttgcgc
      601 gatcacacgc gccgcatgtg gaccggcgtc gtgctctcga tcccgatggt gattctcgcc
      661 atgtcgccga tggtcggcat cgccgaaccg ttcggcctcg cgccgcgcgc gcgcggctgg
      721 atcgaattcg tcctcggcac gcccgtggtg ctctgggtcg gttggccact gctcgccaag
      781 ttctggcgct cggtgcgagg ctggcgcctc aacatgtact cgctgatcgg cctcggcgtg
      841 gggctggcgt acctcttcag tctggtcgcg atcctgtttc ccgagatctt tccgaaggaa
      901 ttccgcgagc acgacggctc ggtcggcacc tacttcgagg cggcgggcgt gatcgtcacg
      961 ctggtgatgg tgggcgaggt gctgcagatg cgcgccatgg ggcagaccag ccaggcgatc
     1021 cggcagcttc tcgagctggc gcccaacctc gcctggcggc tgaaggagga cggcaccgaa
     1081 gagcaggtgc cgctcgattc agtgcacgag ggcgaccgtc tgcgcgtgaa gccgggcgac
     1141 aaggtcccgg tggacggcac ggtgctggag ggcgcgagcc gcatcgacga atccatgatc
     1201 acgggcgagc cggtgccggt ggcgaagaaa tcgggtgacc gcgtgaccgg cgcaacggta
     1261 aacggcagcg ggtcgctcgt catgcgcgcc gaacgcgtcg gttccgaaac gctcctttcg
     1321 cgcatcgtgc acatggtggc cgaggcgcaa cgcaccaagg cgccgatcca gcgcctcgcc
     1381 gacctcatcg ccgcgtactt cgtgcagacg gtggtcgtca tcgccgtcct caccgccctg
     1441 gtgtggtggt tcttcggccc ggagcccgcg ctcacctacg cgcttctcaa ctcggtggcg
     1501 gtgctgatca tcgcctgtcc ctgtgccgtg gggctggcga cgccgatctc aatgaccgtg
     1561 gcgatgggcc gcggcgcggg catgggaatc ctgtttcgca actccgaagc catcgagcac
     1621 atgcgcaaga ttgacacggt ggtcgtggac aagaccggca cgctgacgct cggcaagccc
     1681 gcgctcaccg atttcgcctg cgaaggcatt gaggaggccg aggcgctcgc cctcgtcgcg
     1741 ggcgtggagc agctatccga gcacccgatc gccacggcga tcgtccaggg cgcgaagtcg
     1801 cgcaaggtcg ccccggcgcg cgcggaaggc ttcgacgcgg tgaacggcat gggcgtcgaa
     1861 gcgcacatca acggacgcaa agtgctggtc ggctcgcgcg ccttcctgca gcagcgcggc
     1921 gccgaacccg gcgcgtggga agagcgcgcc gagaaatggc gcggcgaggc gaagaccgtg
     1981 gtcttcttcg ccgtggacgg gcgcacctac ggcatcgccg cggtggccga cccggtgaag
     2041 gacagcaccc ccgaggcgat cgcggcgctg aagcagcgtg gcgtgcgcat tgtcatgctg
     2101 accggcgact ccaagcgcac ggcggcggtg gtcgcgcgcc agctcggcat cgacgaggcg
     2161 gtgggcgaag ttctgcccga gaacaaggcg ctggaagtga agcgcctgca gtccgagggg
     2221 cgcaaggtcg cgatggcggg cgacggcatc aacgacgcgc ccgcactcgc gcaggccgac
     2281 gtcggcatcg ccatgggcac cggcaccgac gtggcgatgg agagcgcggg cgtgacgctc
     2341 gtcaagggcg acctgcgcgg catcgcgcgc gcagcggcac tttctcgcgc caccatgcga
     2401 aatatccggc agaatctcgt gttcgcgttc ggctacaacg cgctcggcat tcccatcgcc
     2461 gcgggcgtcc tttatccggc tttcgggctg ctgctctcgc ccatcatcgc cggcgcggcg
     2521 atggcggcga gctcgctgtc cgtgattacc aacgcgctgc ggctgcggcg cacatcgttc
     2581 taagggggca acatgcccac gactgcaact gcgaagtacg ttttcggcaa gaccgtcggc
     2641 ctcggcttcg atgaagccat cgcccgcgtg accgaggctc tggcgaagga aggcttcggc
     2701 gtgctcaccg agatcgacgt gaaggcgacg ctgaagaaga agctcggcat cgacgtcgcg
     2761 ccctaccgca tcctcggcgc ctgcaacccg cagtacgcgc acaaggcgat cgagttcgag
     2821 ccgcagatcg gggcgctcct gccgtgcaac gtcgtggtgc gagaggaaaa gggcaagacg
     2881 cgcatcgaca tcatggatcc gcaagccgtg ctgcagctcg tcgagcggcc cgagatcgag
     2941 ggcatcgcga ccgaggtccg tcaacggctc gagcgcgtgc tcgcgtcgct ctaagggcgg
     3001 aaagccggcg ggcttgactt tgccatgctg ggaaggtgca gactgagccg aagatcacac
     3061 acgggagcgc ttttatgatc gagctgagcg tacaaggcat gacctgcggt cactgcgtga
     3121 gcgccgtgac gcgggccgta aaaaccgtcg atccggaggc cgatgtccag gtcgacctcg
     3181 gaaccggaaa ggttcgcgtg gacggccgca gttccgccga agcgctgggt aaggcgatcg
     3241 ccgacgcggg ctatccggcc gccgaggcgg ccgcgccgaa gtaaggcggt tgcgcctgaa
     3301 cgggcgcggc tggcctagtg ccggtgcgcg cggtctggcg ccggcttgtc cggcgccgcc
     3361 agcccgtcga ggatcgggca ctcgggccgc tcgtcgccgt ggcagtgctc ggcgagtgcc
     3421 tgcagcgtgc gcttcatcgc ctgcagctcg gcgacgcgcg catcgagcgc cgcgatatgc
     3481 tccaacgcga gcgccttcac cttgctgctc gggcggcgct ggttgcgcca caggccgagc
     3541 aagtgctcga tctgcttgat cgagaagccg aggttgcgcg cctgccggat gaagcgcagc
     3601 gtgtgcacgt cgttgcccga gtaggtgcga taacccgaga aggtgcgcgc cgccttggga
     3661 atcagtccga tgccctcgta gtggcggatc atcttcgccg agacgccgga gtgcttcgcc
     3721 gcctggccga tattcatctg catcatgtcc atcacttggc tcccggtttc cagttgcgca
     3781 gggtgagcgc gttggtcacc acgctcacgc tgctgaacgc catcgccgcg cccgcgatga
     3841 tcgggttcag gaacccgagc gcggcgagcg ggatgccgac cacgttgtag aagaaggccc
     3901 agaacagatt ctggcggatc ttgcggtagg tgcgccgcga cacgccgatc gcgtcggcga
     3961 cgagccgcgg gtcgccgcgc atgagcgtga tgccggcggt gtgcatcgcc acgtcggtgc
     4021 cggtcgccat cgcgatgccg acgtcggctg cggcgagcgc gggcgcgtcg ttgatgccgt
     4081 cgcccaccat ggccaccacg cggcgtttgc cgcgcagggc ggccaccgtc tgcgccttgt
     4141 ccgcgggcag tacttcggcg cgcacctcgt cgatgccgag cgcgcgcgcg gcggccgtgg
     4201 cgctgcccag gttgtcgccg ctcagcatca cggtcttcac gccctgcgcg tgaagctgcg
     4261 ctatcgcctc gcgcgcgccg gccttcaccg cgtcgccgaa cgcgagcagc ccaagaaggc
     4321 gccgcgcacc gtcggtttcc tcggcgagcc acgagatcga gcggccttgc gccgcgagcg
     4381 cccgctcccg gccgctgagc gccgccgtgt ccacgcccag ctcctgcatg agccgcgagc
     4441 tgcccaggta cagacggcgc ccttccacga ccgcctcgac gccacgcccg ggcagcgcct
     4501 tcgagtcgct cgcgacgggc acggaaacgc cgtccgcccc ggcgcgttcc gtgaccgcgc
     4561 gcgcgagcgg atgctggctg ccttgctgca gcgccgccgc cagccgcagg agctcatcgc
     4621 gcgggcccgc cagcggctca aaggcgagca gttgcgggcg tccctcggtc agcgtgccgg
     4681 tcttgtcgaa ggcgaccgtg gtgatcgagt gcgcgatctc gagcgcttcg gcgtccttga
     4741 tcaggatgcc atggcgcgcc gcgacgcccg tgcccgccat gatggcggtg ggcgtggcga
     4801 gccccagcgc gcaggggcag gcgatcacca ggaccgcgac cgcgtagagc acggccgaag
     4861 tccagttgcc cgtcgcgaaa ccccaggcaa gcagcgtcgc cagcgcgatc agcagcacga
     4921 tcggcacgaa cacggcgctc acccggtcca ccaggcgctg gatcggcgcc ttggccgcct
     4981 gcgcgttctc gaccatgcga atgatgcggg cgagcgtgga ttcggcgccc accgcgcgcg
     5041 tcgccacgac aagcaggccc tcggcattga tcgcgccgcc ggtcacgcgc tcgcccgcgc
     5101 ccttggccac cggcaggctc tcgccggtga tgagcgattc gtcgacgtgg ctgcggcctt
     5161 cgcggatctc gccgtccacc gcgatgcgct cgcccgggcg caccaccacg aggtcgccta
     5221 ccgcgatcga tccgaccggc acctctacgt cctttccatt acggcgcacg agcgcgcgct
     5281 cgggccgcag cgcctcgagg gcgcggatcg cggcggtggt ctggcgcttc gctcgcgcct
     5341 ccagccactt gcccagcagg atgaaggtga tcagtgccgc ggcaatctcg aagtagagct
     5401 cgtgcgcgcc gccgccggtg agcaggtgga acaccgagag gccatacgcc gcgctcgtgc
     5461 cgatcgcgac cagcaagtcc atgttgccgc tgaacgcgcg caaggctttc cagcccgcca
     5521 cgtagaacgg cgcgccgagc cagaacagga cgggtgtcga gagcgcgagc tgcagccatc
     5581 cggacagcgt ccacggcgcg cccacggcca gcccgatcat cggaacgacg agcggcagcg
     5641 tgagcacggc cgccaccgcg actcgccacc agtctggaag cgcgtgtgcg gtctgcttcg
     5701 gcggcgcgct ttggcggaga tcgagagggt cggcctcgta gccggccgcg gcgatcgcct
     5761 cgcgcagggc cccgatccca agcccggctg cggcgaccac gtgcgcccgc tcggtggcga
     5821 gattgacctg cgccgagcgc acgccgggta ccttgttcag ggcccgctcg acccggccga
     5881 cgcacgacgc gcacgtcatg ccgcggatcg cgaggtcgta ggcgtcgctg tcgaggtccg
     5941 cgctcgcggc tggcgcggcg ctggtagccg aaatatccat ctccgtctcc ctgagagtcc
     6001 gccgccagtg tgaaggttcc catactggga aggtcaagcc aacccgcgtc tgaggggcat
     6061 gcgatgctgc ctggcccgcc gcgagcccct caccgggttg acagggatca acgcccggca
     6121 aatggaaaac gctagcatgc gccttcgcag cttcgacaga ggttctgcat tgaaggaatt
     6181 cctgcatcgc gcgccgcatt cacgacgccg cttcctcgcc ggtctcgccg gcctgtcggc
     6241 gctgccgctc ctgccagcgc gtggcagcgc gcaagccatg cccgcgctcg tcgaagcgcg
     6301 gctcgtcgct gcgcccaggg aggtgcagct cgtgccggag aaatacccgg ccacgggcgt
     6361 gtgggcctac aacggctcgg tgccgggccc cgtgctgcgc gcgcggcagg gcgagcgcct
     6421 gcgcgtgcac gtggagaacg ccctggccga gcagaccacc gtgcactggc acggcatccg
     6481 cctgcccaat gccatggacg gcgtgccttt catcacgcag cgcccgatcg agccgggcgg
     6541 cggcttcacc tacgagttca cgctgccgga tgcaggcaca ttcttctatc acccgcacca
     6601 gcgcagctac gagcaggtcg gccgcggcct gtcgggcgcg ctgatcgtcg aggagcgcac
     6661 gccccccgag gtcgatcgcg acgtgctgtg ggtgctcggc gactaccggc tcagcgccga
     6721 cgcctcgatc cgcggcggct tcggccactt catggacctg agccatgccg ggcgcatcgg
     6781 caacacggtg acgctgaacg ggcacatgcc ggagtccttt cgcgtgcgcg cgggcgagcg
     6841 ccttcggctg cgattgatca atgcagcccc ggcacgcgtg ttcgcgctgg aattccgtgg
     6901 ccaccggccg tgggtcatcg cgctggacgg gcagcccgta gagccgcacg agccgccggg
     6961 cggtgcggtc gtgctcggcc cggcaatgcg ggccgacctg atcctcgaca tgacggccgc
     7021 gcccggcagc cggcacgccg tgcacgacgg cttctacgcg cgccggccgt acg
//
LOCUS       DBNHKE010000044         6014 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1222604, whole genome shotgun sequence.
ACCESSION   DBNHKE010000044 DBNHKE010000000
VERSION     DBNHKE010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1222604"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="AC16O9_02600"
     CDS             <1..414
                     /locus_tag="AC16O9_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6515537.1"
                     /translation="AGDAFFSRVLKGAFRFVTGLIARKERQNLQVGVVVATIGIRGTH
                     FEGEVIERQEKDGQTVDASARVVLLEPEGSDGGSAIVVENKFGSVVIDQPGFGTEIPD
                     EKSPPSPVRRMQLRTLDNLLRVIRTPGRTGPMRRP"
     gene            complement(411..1343)
                     /gene="egtD"
                     /locus_tag="AC16O9_02605"
     CDS             complement(411..1343)
                     /gene="egtD"
                     /locus_tag="AC16O9_02605"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR6515538.1"
                     /translation="MVPADSFREDVIAGLSQGQKALPPKYFYDAEGSRLFDRICRLKE
                     YYPTRAELALTRAHLADIARFAGRGCELIEYGSGESLKTRLLLRALRPSVYVPVDISH
                     SALAGAAGRLSREFAWLHIFAVVGDFSQPLRLPVFRARPGRRRVVYFPGSTIGNLSTQ
                     EARAFLAMSRAQIGAGGAMLIGVDVKKDANVLHAAYNDARGVTARFNLNLLRRINREL
                     GADFDLRRFHHYAFYNAPLGRIEMHLVSLVRQVVRIGRHRFAFERGETIHTENSHKYA
                     VEEFRALAAQAGFRGAKLWQDRQGLFSLHGLVAD"
     gene            complement(1367..2233)
                     /gene="pbpG"
                     /locus_tag="AC16O9_02610"
     CDS             complement(1367..2233)
                     /gene="pbpG"
                     /locus_tag="AC16O9_02610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR6515539.1"
                     /translation="MRSGERRLHRLEKIVAICALAGVLFVSVGFARAGDGPKLRSSVA
                     LVQDVESGETLYEKNSDTILPIASITKLMTAIVVLERELDLEEQVAVSKEDVRATRSS
                     RVRGRLRPGSILSRDELLMLTLMASENRAAAALARTYPGGTEAFVAAMNAEAGRLGLN
                     DTRFADPTGLSPDNVSSAQDLAGLVRAAHSYPLIREYSTKPSAQVRARGGTVGFRNTN
                     SLVRSDAWDIELSKTGYISAAGRCLVMQMRIASRDLVVILLDSWGKYTRIADARRIRK
                     WLESSAARDPQS"
     gene            complement(2351..4171)
                     /locus_tag="AC16O9_02615"
     CDS             complement(2351..4171)
                     /locus_tag="AC16O9_02615"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABCB family ABC transporter ATP-binding
                     protein/permease"
                     /protein_id="HIR6515540.1"
                     /translation="MAPSPSGPAFPAPARRSDWRVLGLLLPYLWEYRWRVAIALAFLV
                     AAKFANVAVPLVLKEVVDGLDPSLAVLTVPAALLAAYGLLRFSTTLFAELRDVVFVRV
                     VQRAIRRVALGVFRHLHELSLRFHLERQTGGMTRDIERGTRGISQLLNYMIFSIIPVI
                     LEFALVAAVLLAKFDWRFAAITFGAVAVYLTFTFLVSEWRIAIRKRANELDSRANTRA
                     IDSLLNYETVKYFGNEEFEARRYDESLMRYESAATTSEASLGLLNIGQSLIIALAVTA
                     LMFLVAEGVAARALTLGDLVLVNGLLIQLYIPLNFLGMVYREIKQSLADMERMFRLLG
                     ENLEVRDKPGAPDLAPGPAAVEFRDVDFDYEPARRILHGVSLQIPAGKRVAVVGHSGS
                     GKSTLARLLYRFYDVGGGAIRVNGTDIRDVTQASLRAAIGIVPQDTVLFNDTILYNIR
                     YGRPEASEAEVIAAARAAHIHEFIESLPLKYESTVGERGLKLSGGEKQRVAIARAILK
                     DPPILIFDEATSALDSKAERAIQAELERIARGRTALVIAHRLSTVMDADQILVLDHGR
                     IIERGTHGELLGAQGEYARMWALQREAAQAEEVLERAKAA"
     gene            4276..4959
                     /locus_tag="AC16O9_02620"
     CDS             4276..4959
                     /locus_tag="AC16O9_02620"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6515541.1"
                     /translation="MSVRALKPLRETQTRILDAAEELFMQHGFEGASMRMLTAKAGVN
                     LAAVNYHFGSKDALVEAVFRRRLDPMNAERLAALERLELEAAGKALTPQAIIRAFIGA
                     SLRMIEDTRGGGRTFSRLLGRAYTEPAKSIRQLIGGMYAPAMERFKAAFVRALPELPK
                     DELVWRMHFMFGTLAYTLAATDTVQLIAGAKPEDRYDARVLEDRLTAFMSAGLLAPLP
                     GAQEVRIAA"
     gene            4982..>6014
                     /locus_tag="AC16O9_02625"
     CDS             4982..>6014
                     /locus_tag="AC16O9_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6515542.1"
                     /translation="MITFAFATLAALVTLAALRAPIFVWSAAVGVMGASWAVILGFGP
                     ATNAVLGAAFVVAATVLTVPALRRRVLSDRVLAVFRRVLPDMSSTEKEAIDAGTVWWD
                     GDLFSGRPDWERLLATPAPRLSAEEQAFIDGPVEEACAMANDWEVTHERYDLPPRLWQ
                     FLKDKGFFGMIIPKKYGGLGFSALAHSEVVTKLSSRCNALAVTVMVPNSLGPAELLLH
                     YGTEEQKNHYLPRLARGQEIPCFALTSPEAGSDAASIPDYGVVCRGQWQGKEVLGMRL
                     TWDKRYITLGPVATLLGLAFRLYDPEQLLGDKEDLGITCALIPATTPGVNIGRRHLPL
                     NGAFQNGPNS"
BASE COUNT          996 a         2047 c         2058 g          913 t
ORIGIN      
        1 gcaggggacg ccttcttctc gagggtcctc aagggtgcct ttcgcttcgt caccgggctc
       61 atcgcccgca aggaaaggca gaacctgcag gtcggcgtgg tcgtcgccac gatcggcatt
      121 cgcggcacgc acttcgaagg cgaggtaatc gagcgccagg agaaagacgg gcagacagtg
      181 gatgcatccg caagggtggt gctgctggag ccggaaggat ccgacggcgg cagcgccatc
      241 gtggtggaga acaagttcgg cagcgtggtc atcgaccagc ccggtttcgg taccgagatc
      301 cccgacgaaa agagtccgcc gtcgccagtg cgcagaatgc agctgcgcac cctcgacaac
      361 ctgctgcgcg tgattcgaac gccgggaaga acgggaccga tgcgcaggcc ctagtcggcc
      421 accagcccgt gcagggaaaa caggccctgg cggtcctgcc agagcttcgc gcccctgaag
      481 cccgcctgcg cggcgagcgc gcggaactcc tccaccgcgt acttgtggga gttctcggtg
      541 tggatggtct cgccgcgctc gaacgcgaag cgatgccggc cgatgcgcac gacctggcgc
      601 acgagcgaca ccagatgcat ctcgatgcgc ccgagcggcg cgttgtagaa ggcgtaatgg
      661 tgaaagcgcc gcaggtcaaa atcggcgcca agctcgcggt tgatgcgccg tagcaggttc
      721 aggttgaagc gcgccgtgac gccgcgtgcg tcgttgtacg cggcgtgcag cacgttggcg
      781 tccttcttca cgtcgacgcc gatcagcatc gcgccgccgg cgccgatctg ggcgcggctc
      841 atggcgagga aggcgcgcgc ttcctgcgtg ctcaggttgc cgatggtcga gccggggaag
      901 tacacgacgc ggcgccggcc gggccgcgcg cgaaacaccg gcagccgcag cggttgcgag
      961 aagtcgccga ccacggcaaa gatgtgcagc caggcgaact cgcgcgacag gcggcctgcc
     1021 gcgcctgcca gcgccgagtg cgagatatcc acgggcacgt acaccgacgg gcgcagcgcg
     1081 cgcagcagca gccgcgtctt caggctctcc ccgctgccat actcgatcag ctcgcagccg
     1141 cgcccggcaa agcgcgcgat gtcggcaagg tgcgcgcgcg tcagcgcgag ttccgcgcgc
     1201 gtcgggtagt actccttcag gcggcagatg cgatcgaaga ggcggctgcc ctcggcgtcg
     1261 tagaagtact tgggcggcag cgccttctgc ccctgcgaca gtcctgcgat gacgtcctcg
     1321 cggaagctgt ccgcgggaac caccgagccg tagaaacgga tgcggcctag ctttgcgggt
     1381 cgcgcgcggc gctggattcg agccacttgc ggatgcgccg cgcgtcggca atgcgcgtgt
     1441 acttgcccca ggagtcgagc aggatcacga ccagatcgcg cgaggcgatg cgcatctgca
     1501 tcaccaggca gcggccggcg gcgctgatgt agccggtctt ggaaagctcg atgtcccagg
     1561 catccgagcg caccaggctg ttggtgttgc gaaagcccac cgtgccgccg cgcgcgcgca
     1621 cctgcgcgct cggcttggtc gagtactcgc ggatcagggg atagctgtgc gcggcccgta
     1681 ccaggccggc gaggtcctgc gcgctggaga cgttgtcggg ggagagcccg gtcgggtccg
     1741 cgaagcgcgt atcgttcagg ccgagacgcc cggcctcggc gttcatggcg gcgacgaagg
     1801 cctcggtgcc gcccggatag gtgcgcgcca gcgctgccgc cgcgcggttt tcggacgcca
     1861 tcagcgtcag catcaggagc tcgtcgcgcg acagtatcga cccggggcgc agccggccgc
     1921 gcacgcgcga gctgcgcgtc gctctcacgt cctccttgct caccgccacc tgctcctcga
     1981 gatcgagctc gcgctccagc accacgatgg cggtcatcaa cttggtgatc gaggcgatcg
     2041 gcaggatcgt gtcgctgttc ttctcgtaca gcgtctcgcc gctttcaaca tcctgcacca
     2101 gcgcgaccga agagcgcagc ttggggccgt ccccggcgcg cgcgaagccg acgctgacga
     2161 acagcacgcc ggcgagcgcg cagatggcga cgatcttttc aagccgatgc agccggcgtt
     2221 cccctgagcg catgacgatt ctttgagatt tggcccgggc gagtgtaagg gcaggaaacg
     2281 tgaaatgcaa gcaaaaataa gggggtagcc ccggacattc aggaaaaatg tgagctattt
     2341 gccctttctt ctaggcggcc ttggcgcgct cgaggacctc ttcggcctgg gcggcctcgc
     2401 gctgcagcgc ccacatgcgc gcgtactcgc cctgcgcgcc gaggagctcg ccgtgggtgc
     2461 cgcgctcgat gatgcggccg tggtcgagca ccaggatctg gtcggcgtcc atcacggtgg
     2521 agagccgatg cgcaatgacc agggccgtgc ggccgcgcgc gatgcgctcc agctcggcct
     2581 ggatggcgcg ctcggccttg gagtcgagcg cggaggtggc ctcgtcgaag atcaggatcg
     2641 gggggtcctt gaggatggcg cgcgcgatgg ccacacgctg tttctcgccg cccgagagct
     2701 tgagaccgcg ctcgcccacc gtgctttcgt acttgagcgg caggctctcg atgaactcgt
     2761 ggatgtgcgc cgcgcgcgcc gccgcgatca cctcggcttc gctcgcctcg ggccggccgt
     2821 agcggatgtt gtagaggatg gtgtcgttga acagcaccgt atcctgcggc acgatgccga
     2881 tcgccgcccg caggctcgcc tgcgtgacgt cgcgaatgtc ggtgccgttc acgcggatcg
     2941 cgcccccgcc gacgtcatag aagcggtaca gcagccgcgc cagcgtcgac ttgcccgagc
     3001 ccgagtgccc gactacggcg acgcgcttgc ccgccggaat ctgcaagctc accccgtgca
     3061 ggatgcgccg cgccggctcg tagtcgaagt cgacgtcgcg gaactcgacc gccgccgggc
     3121 cgggcgcgag atccggcgcg ccgggcttgt cgcgcacctc gaggttctcg ccgagcaggc
     3181 ggaacatgcg ctccatgtcg gcgagcgact gcttgatctc gcggtacacc atgccgagga
     3241 agttgagcgg gatgtagagc tggatcagca ggccgttgac cagcaccagg tcgccgagcg
     3301 tgagcgcgcg cgccgccacg ccttcggcga cgaggaacat gagcgcggtc accgccagag
     3361 cgatgatcag gctctggccg atgttgagca gcccgagcga ggcctcgctg gtggtggcgg
     3421 ccgactcgta gcgcatcagg ctctcgtcgt agcggcgcgc ctcgaactcc tcgttgccga
     3481 agtacttcac ggtctcgtag ttgagcaggc tgtcgatggc gcgcgtgttg gccctggagt
     3541 cgagctcgtt ggcgcgcttg cggatggcga tgcgccactc ggaaaccagg aacgtgaacg
     3601 tcaggtagac ggccacggcg ccgaaggtga tggcagcaaa gcgccagtcg aacttggcca
     3661 gcagcaccgc cgcgaccagc gcaaactcga ggatcaccgg gatgatcgag aagatcatgt
     3721 agttcagcag ctggctgatg ccgcgcgtac cgcgctcgat gtcgcgcgtc atgccccccg
     3781 tctggcgctc gaggtgaaag cgcagcgaga gctcgtgcag gtgccggaat acgccgagcg
     3841 cgacccgccg gatggcgcgc tgcaccacgc gcacgaagac gacgtcccgc aactcggcga
     3901 acagcgtcgt cgagaagcgc agcaggccgt acgcggcgag cagcgcggcg ggcaccgtga
     3961 ggacggcgag cgacgggtcg agcccgtcca cgacctcctt caggaccaga ggcaccgcga
     4021 cgttcgcgaa cttggccgcc accaggaaag cgagcgctat cgccacccgc cagcggtact
     4081 cccagaggta gggcaggagc agccccagca cgcgccagtc gctgcggcgt gccggggccg
     4141 gaaacgcagg accggaaggg gaaggcgcca tcggcaggcg cggattctaa ggcagcgcgc
     4201 gggcgcgcga accccttgac gcgcaagggg ggagtttcct accattcaaa cgttcgtttg
     4261 aaatcgagac tgaccatgag cgttcgcgcc ctgaagcccc tgagagagac ccagacccgc
     4321 atcctcgacg ccgccgaaga gctgttcatg cagcacggct tcgaaggcgc ctcgatgcgc
     4381 atgctgacag ccaaggcagg cgtcaacctc gccgcggtca actaccactt cggctccaag
     4441 gatgcgctcg tcgaggcggt gttccggcgg cgcctcgatc cgatgaacgc ggagcgtctc
     4501 gccgcgctcg agcggctgga gctggaggcg gcgggcaagg cgctcacgcc gcaggcgatc
     4561 atccgcgcct tcatcggcgc cagcctgcgc atgatcgagg acacccgtgg cggcggccgc
     4621 acgttctcgc ggctgctcgg acgcgcctat accgagccgg cgaaatcgat ccgccagctc
     4681 atcggcggga tgtacgcccc cgccatggag cgcttcaagg cggccttcgt gcgcgcgctg
     4741 cccgagctgc ccaaggacga gctggtgtgg cgcatgcatt tcatgttcgg cacgctcgcc
     4801 tacacgctcg cggccaccga caccgtgcag ctgatcgccg gcgcaaagcc cgaggaccgc
     4861 tacgacgcgc gcgtgctgga ggaccggctc accgcattca tgagcgcggg actgctcgcg
     4921 ccgcttcccg gcgctcagga agtgcgcatc gcggcctgag ccacagcaaa ggacatacac
     4981 catgatcact ttcgcattcg ccaccctcgc cgccctggtc acgctcgccg cgctgcgtgc
     5041 cccgatcttc gtctggagcg ctgccgtcgg cgtgatgggc gcttcctggg ccgtgatcct
     5101 gggcttcggg cccgccacca acgccgtgct cggcgccgcc ttcgtcgtcg cggccacggt
     5161 gctcaccgtg ccggcgctgc gccgccgcgt gctgagcgac cgcgtgctcg cggtcttccg
     5221 tcgcgtcctg cccgacatgt catctacgga aaaagaagcc atcgacgccg gcaccgtgtg
     5281 gtgggacggc gacctgttct cgggccgtcc cgactgggag cggctgctcg cgacgccggc
     5341 gccgcgcctc agcgccgagg agcaggcctt catcgacggc ccggtcgagg aagcctgcgc
     5401 catggcaaac gactgggagg tcacccacga gcgctacgat ctgccgcccc gcctgtggca
     5461 gttcctgaag gacaagggct tcttcggcat gatcatcccg aagaaatacg gcggcctggg
     5521 cttctcggcg ctcgcgcatt ccgaagtcgt gaccaagctg tcctcgcgct gcaacgccct
     5581 cgccgtcacc gtcatggtgc ccaactcgct cgggccggca gagctgctgc tgcactacgg
     5641 caccgaggag cagaagaacc actacctgcc gcgcctcgcg cggggccagg aaatcccctg
     5701 cttcgcgctg accagcccgg aagccggctc ggacgccgct tccatcccgg actacggcgt
     5761 cgtctgccgt gggcagtggc agggcaagga agtgctcggc atgcgcctca cctgggacaa
     5821 gcgctacatc acgctcggac ccgtggcgac gctgctcggc ctcgcgttcc gcctctacga
     5881 tcccgagcag ctgctcggcg acaaagaaga cctcggcatc acctgcgcgc tgatcccggc
     5941 caccacgccg ggcgtgaata tcggccggcg ccacctgccg ctgaacggcg cgttccagaa
     6001 cggccccaat tcgg
//
LOCUS       DBNHKE010000045        12558 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_482521, whole genome shotgun sequence.
ACCESSION   DBNHKE010000045 DBNHKE010000000
VERSION     DBNHKE010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12558
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_482521"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="atpD"
                     /locus_tag="AC16O9_02630"
     CDS             <1..843
                     /gene="atpD"
                     /locus_tag="AC16O9_02630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6515543.1"
                     /translation="DFYHEMIEAGVVKTDNLAESKVAMVFGQMNEPPGNRLRVGLTGL
                     TMAEAFRDEGRDILFFVDNIYRFTLAGVEVSALLGRMPSAVGYQPTLAEEMGRLQERI
                     TSTKTGSITSVQAIYVPADDLTDPSPATTFGHLDATVVLSRDIASLGIYPAVDPLDST
                     SRQVDPNTIGEEHYNTTRAVQATLQRYKELRDIIAILGMDELSPEDKLAVSRARKIQR
                     FLSQPFTVAQNFTGQPGKYVTLKDTIKGFKMIVEGECDSLPEQAFYMVGQIEEAFEKA
                     KTLQ"
     gene            843..1265
                     /locus_tag="AC16O9_02635"
     CDS             843..1265
                     /locus_tag="AC16O9_02635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR6515544.1"
                     /translation="MAAATIHVDVVSAEEAIFSGEAEFVVLPGEVGELGIYPRHTPLI
                     TRIRPGAVRIMPAGGGDEQLIFVAGGILEVQPKVVTVLADTAIRGADLDEAKANEALK
                     KAEEARAKAQDKQEIAAVESELAMLAAQLAAIRKLRKK"
     gene            1281..2018
                     /locus_tag="AC16O9_02640"
     CDS             1281..2018
                     /locus_tag="AC16O9_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR6515545.1"
                     /translation="MVLPARNRTSRAAQGGFTLVEIAIVLVVIGLLLGAIFKGQELIT
                     QARVKSVIADFSNLSVAYHGYFDRYGALPGDDPNAGTRWAAAPAGTSGDGDGQVAGTY
                     NNGNAACVTSVESCSWWDHLRRAGFVSGSGATPPLNVAGGLTGVQVGDGTAPTYGPVL
                     GGPAGAGGLRVLIQCSANLPEKIAIAVDVQFDDGARTSGTVRGMLQSAPNPAIAVDAT
                     APAAGGAGSYVETGTNVYTLCRAMHAP"
     gene            complement(2015..2338)
                     /locus_tag="AC16O9_02645"
     CDS             complement(2015..2338)
                     /locus_tag="AC16O9_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016130.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR6515546.1"
                     /translation="MNHLRANGFRVKPQDVSNPALYRSRYGVPHALGSCHTAVIEGYA
                     IEGHVPVREIKRLLKERPKAIGLAVPGMPAGSPGMEGPRRDPYDVLLILPDGSSRVYA
                     RYGTR"
     gene            complement(2728..2952)
                     /locus_tag="AC16O9_02650"
     CDS             complement(2728..2952)
                     /locus_tag="AC16O9_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515547.1"
                     /translation="MQDEQRTASTWRIGFDLAVFVVLAVIAGMVAAMTLAGIAILLTG
                     AGSSAPAARPEAPVQAPAARTMVAHAARVH"
     gene            3105..3794
                     /gene="pdsR"
                     /locus_tag="AC16O9_02655"
     CDS             3105..3794
                     /gene="pdsR"
                     /locus_tag="AC16O9_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HIR6515548.1"
                     /translation="MSRRIAIVEDDPAIRANYAEALRKHGFEVSAYGARAEALAAMRA
                     RLPDLAVIDIGLGSDADAGFELCRELRALSASLPIIFLTARDSDFDVVSGLRLGADDY
                     LTKDVSLPHLLARIAALFRRAELAREPAAAEETLERGALALDLKRFSVAWDGKPVPLT
                     LTEFWMVHALARHPGHVKSREQLMREANLVVDDGTVTSHVKRIRRKFQGVDPGFEHIE
                     TAYGMGYRWRE"
     gene            3791..5446
                     /locus_tag="AC16O9_02660"
     CDS             3791..5446
                     /locus_tag="AC16O9_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6515549.1"
                     /translation="MKRRFALGLRAQLALAALALLALPWAGVSFVNELERTLLEGQER
                     GLLATARAVATALHDRPQLLAAAPARDDAVRREAELELQRIAQSEGRLPQAAIAQLPL
                     AREAERAARQAQEEIAAILRGVQRTESRLWVVNRRYQVLALAGSLRRAADAAPAEGVL
                     RGAWRRLAGWLIERPREDFAEAVPDDVLATGRDIASALQGTPGTRTRLTPDGLAVVMS
                     AAHPVWNGNEVAGAVVVEETTNPIASLRSDALERLLLLTLAAFAGAAAILIAFATRLS
                     QRIRRLRDEAESAIDARGRLARIAAGSDAGDEIGDLSRSFSAMLARLAQHHVYLESMA
                     GRLSHELRTPIAVVRSSLENLKLAATPDEMRTYVARAEEGLARLNTILQRMSEATRLE
                     QALRTAERERYDLGAVVRGCVEGYRLAYAATPFELEAPARALEVSGSPDLGAQMLDKL
                     VDNAVDFSPPGAAVRVRLQDQGRFALLAVQNQGPALPADMRARLFESMVSVRETRAGA
                     TPHLGLGLYVARLIAEFHGGAIEATDAPGGDGVVVRVHLPLTR"
     gene            5457..6239
                     /locus_tag="AC16O9_02665"
     CDS             5457..6239
                     /locus_tag="AC16O9_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR6515550.1"
                     /translation="MKPLTDLRDATRLRGLLFDLDDTLSTDGRLTAEAYAALERLQHA
                     GRRTVLVTGRPAGWCDHIARMWPVHAVVGENGAFYFRYDGGRLRRRFVDDDAARAAKR
                     ARLGAIAERILAAVPGSALASDQGYRETDLAIDYCEDVTPLPLGEAERIAALMRETGL
                     TAKVSSIHVNGWFGDYDKLATAKLLFAEVFDANLDAVQAQYAYAGDSPNDAPMFAYFE
                     NAVGVANVARFAGRLAAAPKYVTRREAGAGFAELAARLLGTA"
     gene            6239..7033
                     /locus_tag="AC16O9_02670"
     CDS             6239..7033
                     /locus_tag="AC16O9_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6515551.1"
                     /translation="MEWWWAYLAIGAAVGFLAGLLGIGGGMVMVPMLFFVFSAKGFPQ
                     EQLMHFALATGLATIAFTSLSSVRAHHRHQSVDWRVAGAMAPGIVAGSLGAALVAGFV
                     PTRPLAVFFTAFMFYASAQMFYEAKPVPTRGLPGAAGLIAVGAGIGGISSILAAGGAF
                     LSIPFLLRCNVPLRRAIGTAAANGFPIAVAGTIGYVLHGLRAPDLPAGSLGFVYLPAL
                     GLIVAASIPIAPLGARVAHRLPVRRLRVAFSLMLLALALRMLANLW"
     gene            7046..8269
                     /locus_tag="AC16O9_02675"
     CDS             7046..8269
                     /locus_tag="AC16O9_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6515552.1"
                     /translation="MAGPLAGIRVLDLSRVLAGPWAGQNLADLGAEVIKIERPGAGDD
                     SRAFGPPWVKDSQGRDTKDSAYYTSANRGKKSVTVNLSQPEGQALVRDLAKKSDVLLE
                     NYKYGDLQRYGLGYDDLKAINPRLIYCSVTGFGQTGPYKERPGYDFMIQGMGGMMSVT
                     GEPDGAPGGGPQRAGVPIADIITGMYASIAICAALAHRAQSGAGQHLDLALLDSQIAL
                     LAYQNTNYFATGKPPARIGNLHPNIVPYQPFRASDGEVILACGNDNLYRKFCEAAGCP
                     ELASDARFLTNGKRVENRKELTRLLGEIFAKRTKRDWVALLDAAGVPNGPINDIAQVF
                     EEPQVKARGVKVEVAHPVAGRLAMVASPMRFSGTPLEHKTPPPLLGEHTDEILQEILG
                     KKPEEIARLREADVL"
     gene            complement(8276..9175)
                     /locus_tag="AC16O9_02680"
     CDS             complement(8276..9175)
                     /locus_tag="AC16O9_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6515553.1"
                     /translation="MAVTKETLDNLNGAQKPRARWLQAVYDFTWQRPLGAIGAVIIVV
                     MALVALTADWLAPYDALATDYAAMLSAPHGAHWLGTDAFGRDVFSRIIYGSRTALIVG
                     LGASFIGATLGALIGVASAYFGGRVDLIVQRIMDILISFPLIILALAVVAILGTGLEN
                     VILAIAVPMIPRCARVVRSSALAVREMPYVDAARAAGYTDARIVLRHMLPNVMAPYLI
                     MLTAFLSQAILLEASLSFLGLGVQEPVAAWGLMLRGAAIEFAETAPWMAIFPGLAISL
                     AVFAFNLFGDSLRDALDPRLRTQ"
     gene            complement(9178..10131)
                     /locus_tag="AC16O9_02685"
     CDS             complement(9178..10131)
                     /locus_tag="AC16O9_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6515554.1"
                     /translation="MIQYILKRFLLMIPTLFGVAVLIFLLLRVVPGDVVEIRFAAQGQ
                     YSSEEVLALERARLGLDKPLWQQFTTWMGGLLRLDFGLSMWTGAPITEEIKLRFELSL
                     QLAIMATVVATLLAIPLGILAAIKQDTWIDYAVRVFSIAGLAMPSFWLGILMILAMLI
                     VFKWLPPMVYTPFWVNPWENLAQLIWPALAVGYRYSAVATRMMRSAMLEVLREDYIRT
                     ARAKGLWLKLILTRHAMKNAMLPVLTVIGLEFAFLMGGLVVTEQVFNLNGLGMLFVEA
                     IARRDYTLTQALVLIVAAVFIFVNFATDIAYAWLDPRIRYR"
     gene            complement(10189..11784)
                     /locus_tag="AC16O9_02690"
     CDS             complement(10189..11784)
                     /locus_tag="AC16O9_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6515555.1"
                     /translation="MKNRLASLLAVVASATLAFPAQAQSPRYGGELVFTVPSEMPSYD
                     GHREQTFGLIHPIAPHYNTLLRVDPNDPTGTTPVGDLAESWTVSSNQLIYTFKLRRGV
                     KFHDGSDMTSRDVKASYDKIINPPEGVASVRKATYLVVEEVEAPDAYTVRFKLKWPSG
                     AFLQSLASPWNFIYKADILAKDMHWYEKNIMGTGPFTFVEHVKGSHWVGKKNPNYWNK
                     GKPYLDGYRALFVRSHSAQVAAVRGERSHIQFRSFTPADKETLSTAMGDKIKFQESAW
                     DCILLVAINHTKKPFDDRRVRKALTLALDRWGASKTLNKIAIVQDVAGIQVPGTPWAT
                     PPEELKKLAGYWPDIDKSRAEARRLLKEAGVPEGFSFTFHNRGTPMPYEPLAIWLIDQ
                     WRQIGLNVKQQVIEPAAYYGVLRKPDAFEVAMDFQCGYIVEPDLDIYKFISNDKNHDN
                     YSYYTDRVLDDLYEKQSRATSKEERTKYLRAFERRLLDEEAHYLMTLQWHRIIPQNAK
                     VHGWQVTPSHYLNNQLDTVWLSE"
     gene            complement(11885..>12558)
                     /locus_tag="AC16O9_02695"
     CDS             complement(11885..>12558)
                     /locus_tag="AC16O9_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515556.1"
                     /translation="PYSSLNPRMTVGQILAEPLKVHRIVPDKGQRELRVRELLVQVGL
                     LPQHARRYPHQLSGGQRQRVGIARALATEPALIVCDEPVSALDVSIQAQIINLLEELQ
                     SRLGLTYLFIAHDLSVVRHISDRVAVMYLGKIAELADRNALYEEPIHPYTQALLSAVP
                     IPDPKIEAKRKRMVVRGEVPSPLNPPSGCVFHPRCPIAVARCSAEVPPLREIRLGHWA
                     ACHLA"
BASE COUNT         2041 a         4173 c         4336 g         2008 t
ORIGIN      
        1 gacttctatc acgagatgat agaagcgggc gtcgtcaaga ccgacaacct tgccgaatcc
       61 aaggtggcga tggtgttcgg ccagatgaac gagccgccgg gcaaccgcct gcgcgtgggc
      121 ctcaccggcc tcaccatggc cgaagcgttc cgcgacgaag gccgcgacat cctgttcttc
      181 gtcgacaaca tctaccggtt cacgctcgcc ggcgtggagg tctccgcgct gctgggccgc
      241 atgccctccg ctgtgggcta ccagcccacg ctcgccgagg aaatggggcg gctgcaggag
      301 cgcatcacgt cgaccaagac cggctcgatc acctcggtgc aggcgatcta cgtgccggcg
      361 gacgacctga ccgacccgtc gcccgccacg accttcggcc atctcgacgc cacggtggtg
      421 ctgtcgcgcg acattgcgtc gctcggcatc tacccggcgg tcgacccgct cgactcgacc
      481 tcgcgccagg tggacccgaa caccatcggc gaagagcact acaacaccac ccgcgcggtg
      541 caggcgacgc tgcagcgcta caaggaactg cgcgacatca tcgccatcct cggcatggac
      601 gaactgtcgc ccgaggacaa gctggcggtg tcgcgcgcgc gcaagatcca gaggttcctc
      661 tcgcagccgt ttacggtggc acagaacttc accggccagc cgggcaagta cgtgaccctg
      721 aaagacacga tcaagggctt caagatgatc gtcgagggcg agtgcgacag cctgcccgag
      781 caggcgttct acatggtcgg gcagatcgaa gaagcgttcg agaaagcgaa gacccttcag
      841 taatggccgc cgccaccatc cacgtcgacg tcgtcagcgc cgaggaggcg atcttctcgg
      901 gcgaggcgga gttcgtcgtc ctgccgggcg aggtgggcga gctcggcatc tacccgcgcc
      961 acacgccgct catcacccgc atcaggccgg gcgcggtgcg catcatgccc gcaggcggcg
     1021 gcgacgagca gctgatcttc gtcgcggggg gcatcctcga ggtgcagccg aaagtggtca
     1081 ccgtgctcgc cgacacggcc atccgcggcg cggacctcga cgaggcgaag gcgaacgagg
     1141 ccctgaagaa agccgaggaa gcgcgcgcca aggcgcagga caagcaggag atcgccgccg
     1201 tcgagagcga gctggcgatg cttgccgcac aactcgcggc gatccgcaag ctacgcaaga
     1261 agtaggctgg gcggcctgcg atggtcctgc ccgcgcgcaa tcgcacctcg cgcgcagcgc
     1321 agggcggctt tacgctggtc gagatcgcga tcgtgctggt ggtcattggc ctactgctcg
     1381 gggccatctt caaggggcag gagctgatca cccaggcgcg cgtcaagagc gtgatcgccg
     1441 acttctccaa cctatcggtg gcctatcacg gctacttcga ccgctatggc gcgctgcccg
     1501 gcgatgaccc gaacgcgggg acgcgctggg cggcggcacc ggcgggcacc agtggagacg
     1561 gcgacggcca ggtggcgggc acctacaaca acggcaatgc cgcgtgcgtg acgagcgtgg
     1621 aaagctgcag ctggtgggat cacctgcggc gcgccggctt cgtatcgggt tcgggcgcga
     1681 cgccgccgct caatgtggcc ggtggcttga cgggcgtgca ggtaggcgac ggcacggcgc
     1741 cgacctacgg gccggtgctg ggcgggccgg cgggcgctgg aggtcttcgc gtactgatcc
     1801 aatgctcggc gaacctgccc gagaagatcg cgatcgccgt cgatgtgcaa ttcgatgacg
     1861 gcgcgcgcac cagcggcacg gtgcgcggca tgttgcagtc ggcgccaaat ccggcgatcg
     1921 ccgtcgatgc cactgcgccg gcggctggtg gcgcgggcag ctacgtcgag accggcacca
     1981 acgtctacac gctgtgccgt gcgatgcacg cgccctagcg cgtgccgtag cgcgcgtaga
     2041 ccctggaact cccgtcggga agaatgagca gcacgtcgta gggatcgcga cgggggccct
     2101 ccatgcccgg cgagccggcc ggcatcccgg gtacggcaag gccgatcgcc ttcggacgct
     2161 ccttcagaag ccgcttgatc tcgcgcaccg ggacgtgacc ttcgatcgca tagccctcga
     2221 tcacggcggt gtggcacgag cccagagcgt gcggcacgcc gtagcgcgag cggtagagcg
     2281 ccgggttact gacatcctgc ggcttcacac ggaagccgtt ggcccgcagg tgattcaccc
     2341 acgagccgca gcagcctcag gtcgcttctt tgtagacggt gatctcttct gccgcccagg
     2401 ttgccgccgc ccaaagcgac agcaccgcgg ccagcagcaa tgcctgtaga ttcgttctca
     2461 tgttctgcct cgcttgaggg tcaagatcag cgcccattaa actgcgcccg cggcgcaccc
     2521 ggattgatct gcgtcaagcg cccctatctt ccgggcttgt gcaccacggc gcagtgcgcc
     2581 caaggtcttc ggcatgtgcg tgttttcccg gccgcaatgt gcgcaatccg ctcgcgggat
     2641 gtcaagccgc cacttgccga agcgggcgag cggggagggc ggccggtgga ggaggctggc
     2701 cccgcgtccc gcgctcgccg ccgcggttca gtgcacgcgc gctgcgtggg ccaccatggt
     2761 gcgcgccgcc ggcgcctgca ccggtgcctc gggccgcgcc gcgggggcgc tcgatccggc
     2821 gccggtgagc aggatggcga tgccggcgag cgtcatcgct gccaccatgc ccgcaatgac
     2881 ggcgagcacg acaaagaccg cgaggtcgaa accgatgcgc caggtgctgg cggtgcgttg
     2941 ctcgtcttgc atgcgtggga tctccgtggg acgttgcgtg taggcgcatt acgccggacc
     3001 cccgcgacgt tgccacggcg tgctgcgggc aagctggcga tcaattctgg caatcccggg
     3061 gcagccaccg cccctgcggc ggccttgggc tatagtcgtt cgccatgtcc cgccgcattg
     3121 ccatcgtcga agacgatccg gccatccgcg ccaattacgc cgaggcgttg cgcaagcacg
     3181 gcttcgaggt aagcgcctac ggcgcgcgcg ctgaggcgct cgcagcgatg cgcgcgcgcc
     3241 tgcccgatct ggcggtgatc gatattggcc tgggaagcga tgccgatgcc ggcttcgagc
     3301 tgtgccgcga gctgcgcgcg ctgtcggcgt cgctgccgat catctttctc accgcgcgcg
     3361 atagcgattt cgatgtcgtc tccggcctgc gcctcggggc cgacgactac ctgacgaagg
     3421 atgtgagcct gccgcacctg ctcgcgcgta tcgcggcgct gtttcgtcgg gccgagctcg
     3481 cgcgcgagcc cgcagccgcg gaggagacgc tcgagcgcgg cgcgctcgcg ctcgacctga
     3541 agcgcttcag cgtcgcctgg gacggaaagc ccgtgccgct cacgctcacc gaattctgga
     3601 tggtgcacgc gctggcgcgc catcccgggc acgtgaagag ccgcgagcag ctgatgcgcg
     3661 aggcgaacct ggtggtggac gacggtaccg ttacctcgca cgtcaagcgc atccgccgca
     3721 agttccaggg cgtcgatccc ggcttcgagc acatcgaaac cgcctacggc atgggctacc
     3781 gttggaggga gtgaaacggc gcttcgcgct cggactgcgc gcgcagctcg cgctcgccgc
     3841 gctggcgctg cttgcgctgc cctgggcggg cgtgagcttc gtgaacgaac tggagcgcac
     3901 gctgctcgag ggccaggagc gggggctgct cgcgaccgcg cgcgcggtgg ccaccgcgct
     3961 gcacgaccgg ccgcagctcc ttgcggcggc gccggcgcgc gacgatgccg tgcgccgcga
     4021 ggccgagctc gagctgcaac gcatcgcgca gagcgaaggc cggctgcccc aggccgcgat
     4081 cgcgcagctg ccgctcgcgc gcgaggccga gcgggcggca cgccaggcgc aggaggagat
     4141 cgccgccatc ctgcgcggcg tgcagcgcac cgagtcgcgg ctgtgggtgg taaaccggcg
     4201 ctaccaggtg ctcgccctcg cgggcagctt gcgccgggct gcggatgcgg cgcccgcgga
     4261 gggcgtcctg cgcggggctt ggcggcgcct ggcgggctgg ctgatcgagc gtccgcgcga
     4321 ggacttcgcc gaggcggtgc ccgacgacgt gctcgccacg ggacgcgaca tcgcctcggc
     4381 gctgcagggc acgccgggga cccgcacccg cctgacgccc gacggcctcg cggtggtgat
     4441 gtctgcggcg cacccggtgt ggaacggcaa cgaggtggcg ggcgctgtgg tggtcgagga
     4501 gaccaccaac ccgatcgcca gcctgcgcag cgacgcgctc gagcggctgc tgctcctcac
     4561 gctggccgcg ttcgcgggcg ccgcggcgat cctcatcgcc ttcgccacgc gcctgtcgca
     4621 gcgcatccgg cgacttcgcg acgaggccga gagcgccata gatgcgcgcg ggcggctcgc
     4681 ccgcatcgcc gccggctcgg acgcgggcga cgagatcggc gacctgtcgc gcagtttctc
     4741 ggcgatgctg gcgcggctcg cgcagcacca cgtctatctg gaaagcatgg ccggccggct
     4801 gtcgcatgag ctgcgtacgc cgatcgccgt agtgcggtcc tcgctggaga atctgaagct
     4861 cgcagccacg cccgatgaga tgcgtaccta cgtggcacgg gccgaggaag ggctcgcccg
     4921 gctgaacacc atcctgcaga ggatgagcga agccacgcgc ctcgagcagg cgctgcgcac
     4981 cgcagagcgc gagcgctacg acctcggcgc cgtggtgcgc ggctgcgtcg agggctaccg
     5041 gcttgcctac gccgcgacgc cgttcgagct cgaggcgccg gcgcgcgcgc tggaagtctc
     5101 gggctccccc gatctcggcg cgcagatgct cgacaagctg gtggataacg cggtcgattt
     5161 ctcgccgccg ggcgccgccg tacgcgtgcg gctgcaggac cagggccgct ttgcgctgct
     5221 cgcggtgcag aaccaggggc ccgcgctgcc tgcggacatg cgcgcgcgcc tgttcgagtc
     5281 gatggtgtcg gtgcgcgaaa cgcgtgccgg cgccacgccg cacctcggac tcggcctgta
     5341 cgtcgcgcgc ctgatcgcgg agttccacgg cggcgccatc gaggcgaccg acgcccccgg
     5401 gggcgacggc gttgtcgtgc gcgtgcactt gcccctgacg cgatagagtc tcgtccgtga
     5461 agcccctgac ggacctgcgc gacgcgacgc gcctgcgcgg cctgttgttc gacctcgacg
     5521 acaccctgag cacggatggc cgtctcacgg ccgaggccta cgccgcgctg gagcgcctgc
     5581 agcacgccgg ccggcgcact gtcctggtca ccggccggcc cgcgggctgg tgcgaccata
     5641 tcgcgcgcat gtggccggtg cacgccgtgg tcggggagaa cggcgcgttc tactttcgct
     5701 acgacggcgg caggctgcgc cggcgcttcg tcgacgatga cgcggcgcgc gcggcgaagc
     5761 gcgcccggct cggcgccatc gccgagcgca tcctggcggc ggttccgggc agcgcgctcg
     5821 cttcggacca gggctatcgc gagaccgatc tggcgatcga ctattgcgag gacgtgacgc
     5881 cgctgccgct cggcgaggcc gagcgcatcg ccgcgctcat gcgcgagacg gggcttaccg
     5941 cgaaggtgag ctcgatccac gtcaatggct ggtttggcga ctacgacaag ctggcgacgg
     6001 cgaagctgct gtttgccgag gtgttcgacg cgaacctcga tgcggttcag gcgcagtacg
     6061 cctacgccgg cgactcgccg aacgatgcgc cgatgttcgc ctacttcgag aacgcggtgg
     6121 gtgtggccaa cgtcgcgcgc ttcgcgggcc gcctggccgc ggcacccaag tacgttacgc
     6181 ggcgcgaagc cggtgccggc tttgccgagc tcgcggcccg gcttctcggg accgcctgat
     6241 ggaatggtgg tgggcttatc tggcgatcgg cgcggccgtg ggtttcctgg cgggcctgct
     6301 cggcatcggc ggcggcatgg tgatggtgcc gatgctgttc ttcgtcttca gcgcgaaggg
     6361 cttcccgcag gagcaattga tgcattttgc gctggccacc ggcctggcaa ccatcgcctt
     6421 tacttcgctt tccagcgtgc gcgcacatca tcggcaccag agcgtcgact ggcgcgtggc
     6481 aggcgccatg gcgcccggca tcgtcgccgg atcgctcggc gccgcgctgg tggcggggtt
     6541 cgtgccgacg cgcccgctgg cggtgttctt caccgctttc atgttctacg cctccgcgca
     6601 gatgttctac gaggcgaagc cggtgcccac gcgcggcctg ccgggcgccg cggggttgat
     6661 cgcggtgggc gcgggaatcg gcgggatctc gagtattctc gcggcgggcg gcgcgttcct
     6721 gtcgatcccg tttctcctgc gctgcaacgt gccgctgcgg cgcgcgatcg ggacggcggc
     6781 ggcgaacggc tttcccatcg cggtggcggg caccatcggc tatgtgctgc acgggctgcg
     6841 cgcaccggac ctgcccgccg ggagcctcgg cttcgtctac ttgccggcgc tcgggctgat
     6901 cgtcgcggcg agcatcccga tcgcgccgct cggcgcgcgc gtcgcacatc ggcttccggt
     6961 gcggcgcctg cgcgtggcgt tctctctcat gctgctcgcg ctcgcgctgc gcatgctggc
     7021 aaacttgtgg tgaaggagga ttgaaatggc cggacctttg gcaggcatcc gcgtgctgga
     7081 tctgtcccgc gtgctcgcgg ggccctgggc ggggcagaac ctcgccgacc tcggcgccga
     7141 agtgatcaag atcgagcgcc ccggggccgg cgacgattcg cgcgccttcg gcccgccctg
     7201 ggtgaaggac tcccagggtc gcgacacgaa agactccgcg tactacacgt ccgcgaaccg
     7261 cggcaagaag tcggtgacgg tgaacctgtc gcagcccgag ggccaggcgt tggtgcgcga
     7321 tctggcgaag aaaagcgacg tactgctcga gaactacaag tacggcgatc tgcagcgcta
     7381 tgggctgggg tacgacgacc tgaaggcgat caacccgcgg ctgatctatt gctcggtcac
     7441 cggcttcggg caaacggggc cgtacaagga gcgccccggc tacgacttca tgatccaggg
     7501 catgggcggg atgatgagcg tcaccggcga gcccgacggc gcgccgggcg gcggtccgca
     7561 gcgcgccggc gtgccgatcg ccgacatcat caccggcatg tacgcctcga tcgcgatctg
     7621 cgccgcgctc gcgcaccgcg cgcagtccgg agccggccag cacctcgacc ttgcgctgct
     7681 cgattcgcag atcgcgctgc tcgcctacca gaacaccaat tacttcgcga ccggcaagcc
     7741 gcccgcgcgc atcggcaacc tgcacccgaa catcgtgccc tatcagccgt ttcgcgcgag
     7801 cgacggcgag gtgatcctcg cctgcggcaa cgacaacctg taccgcaagt tctgcgaggc
     7861 ggcaggctgt cccgagctgg cgagcgatgc gcgctttctc accaacggca agcgcgtcga
     7921 gaaccgcaag gagctcaccc ggctgctcgg cgagatcttc gccaagcgca ccaaacggga
     7981 ctgggtcgcc ctgctcgacg ccgcgggcgt gcccaacggc ccgatcaacg acatcgcaca
     8041 ggtgttcgaa gagccgcagg tgaaggcgcg gggcgtcaaa gtcgaggtcg cgcatccggt
     8101 cgccggccgg ttggcgatgg tggcgagtcc gatgcgcttc tcgggcacgc cgctcgagca
     8161 caagaccccg ccgccgcttc tgggcgagca caccgacgag atcctgcagg aaatactggg
     8221 caagaagccc gaggagatcg cgcgcctgcg cgaggcggat gtgctctaga gccggttact
     8281 gggtgcgcag gcgcgggtcg agcgcgtcgc gcagcgaatc accgaacagg ttgaaggcga
     8341 acaccgccag gctgatggcg agtcccggga agatcgccat ccacggcgcg gtctcggcga
     8401 actcgatcgc agcgccccgc agcatcaggc cccaggccgc gaccggctcc tgcacgccca
     8461 ggccgaggaa ggagagcgaa gcctccagga ggatggcctg gctgaggaag gcggtcagca
     8521 tgatcaggta cggcgccatc acgttcggca gcatgtggcg cagcacgatg cgcgcgtcgg
     8581 tgtagccggc ggcgcgcgcc gcgtcgacgt atggcatttc gcgaaccgcg agggcgctcg
     8641 agcgcaccac gcgtgcacag cgcgggatca tcgggaccgc gatcgcaagg atgacgtttt
     8701 ccagaccggt gcccagaatc gccactacgg caagcgccag gatgatgagc ggaaacgaga
     8761 tcaggatatc catgatgcgc tgcacgatca gatcgacccg gccgccaaaa taggcgctgg
     8821 ccacgccgat caaagcgccg agcgtcgcgc cgatgaacga cgcccccaga ccgacgataa
     8881 gcgcggtgcg cgaaccgtaa ataatgcgcg agaacacgtc gcgaccgaag gcatcggtgc
     8941 cgagccagtg cgcgccgtgc ggcgccgata acatggccgc atagtcggtg gcgagcgcgt
     9001 cgtatggcgc gagccagtcc gcggttagcg cgaccagcgc catgactacg atgatgacag
     9061 cgccgattgc gccaagcggc ctttgccagg tgaagtcgta gaccgcctgc agccagcgtg
     9121 cacgcggctt ttgcgcgccg ttgaggttgt cgagggtttc tttggtgacg gccatggcta
     9181 gcggtagcgg atgcgcgggt cgagccacgc gtaggcgatg tctgtcgcga aattgacgaa
     9241 gatgaacacc gccgccacga tcagcaccag cgcctgcgtg agcgtgtaat cgcgccgcgc
     9301 gatcgcctca acgaacagca ttcccaggcc gttcaggttg aacacctgct cggtcaccac
     9361 gaggccgccc atcagaaacg cgaactccag tccgatcacg gtgagcaccg gcaacatcgc
     9421 gttcttcatc gcatggcggg tgagaatgag cttcaaccac agccctttgg cgcgcgcggt
     9481 acggatgtaa tcctcgcgca ggacctcgag catcgcggag cgcatcatgc gcgtcgccac
     9541 cgcggagtag cggtagccga ccgcgagagc tggccagatc agctgcgcga ggttctccca
     9601 agggttcacc cagaaaggcg tatagaccat gggcggcagc cacttgaaga cgatgagcat
     9661 cgccagaatc atcaggatgc cgagccagaa cgatggcatg gccagtccgg cgatcgaaaa
     9721 cacccgaacc gcgtaatcga tccacgtgtc ctgcttgatt gccgcgagga tgccgagcgg
     9781 gatggcgagc agcgtggcca ccacggtcgc catgatcgcg agctgcaggg atagctcgaa
     9841 acgcagcttg atctcctcgg tgatcggcgc tccggtccac atggacagac cgaagtcgag
     9901 acgcagcaat ccccccatcc aggtggtgaa ctgctgccac agaggcttgt cgagaccgag
     9961 ccgcgcgcgc tcgagcgcca gcacctcttc ggagctgtac tgcccctggg ccgcgaagcg
    10021 gatctcgacc acgtctccgg gaaccacacg tagcagcaga aagatgagga ccgcgacccc
    10081 gaacagggtc gggatcatca gtagaaaccg cttcaagatg tactggatca tcgcgcgaac
    10141 cgccggcggc aggttgctcc cgccgccggc cgtcagcacc ctaccgagtt actccgacag
    10201 ccagaccgta tcgagctggt tgttgaggta gtggctcggc gttacctgcc agccgtgcac
    10261 cttcgcgttc tgcgggatga tccggtgcca ctgtagcgtc atgaggtagt gcgcttcctc
    10321 gtccagcaga cgacgctcga acgcgcgcag gtatttcgta cgttcctcct tgctggtcgc
    10381 gcgggactgc ttctcataca agtcatccag cacgcggtcg gtgtagtagc tgtagttgtc
    10441 gtgattcttg tcgttcgaga tgaacttgta aatgtcgagg tcgggctcga cgatgtagcc
    10501 gcactggaag tccatcgcca cctcgaatgc atcgggcttg cgcagtacgc cgtagtaggc
    10561 cgcaggctcg attacctgct gcttcacgtt aaggccgatc tggcgccact ggtcgatcag
    10621 ccagatcgcg agcggctcgt agggcatcgg cgtgccgcgg ttgtggaacg tgaacgagaa
    10681 gccctcgggg acgcccgctt cctttagcag gcgccgcgcc tctgcacggg acttgtcgat
    10741 atcaggccag tacccggcga gcttcttcaa ctcctccggc ggcgtcgccc acggcgttcc
    10801 cggtacctgg atgcccgcca cgtcctgcac gatcgcgatc ttgttcaggg tcttggaggc
    10861 accccagcga tcgagggcga gcgtcaatgc cttgcgcaca cgcctgtcgt cgaagggctt
    10921 cttggtgtga ttgatcgcca cgagaagaat gcagtcccac gcgctttcct ggaacttgat
    10981 cttgtcgccc attgccgtgg acaaagtttc cttgtccgct ggcgtgaagc tgcggaactg
    11041 gatgtgcgag cgctctccgc gcaccgcagc cacctgtgcc gagtgcgagc gcacgaacag
    11101 cgcgcgatag ccgtccaggt agggcttgcc cttgttccag tagttcgggt tcttcttgcc
    11161 gacccagtgc gagcccttga cgtgctcgac gaacgtgaac gggccagtgc ccatgatgtt
    11221 cttctcgtac cagtgcatgt ccttcgccag gatgtcggcc ttgtagatga agttccaagg
    11281 cgaagcgagc gactgcagga acgcacccga aggccacttc agcttgaagc ggaccgtgta
    11341 ggcatcgggc gcctcgacct cttccaccac caggtacgtc gccttgcgca cgctggcgac
    11401 gccttcgggc gggttgatga tcttgtcgta ggacgccttt acgtcccgcg aggtcatgtc
    11461 ggagccgtcg tggaacttga cgccccggcg cagcttgaac gtgtaaatca gctgattgct
    11521 cgagacggtc cagctttcag cgaggtcccc caccggcgtg gtgccagtcg ggtcgttggg
    11581 gtcgacgcgc agcagcgtgt tgtagtgcgg cgcgatcgga tggatcagac cgaaggtctg
    11641 ctcgcggtgg ccgtcgtagg acggcatctc cgagggcacg gtgaagacga gttccccacc
    11701 gtaccgggga ctttgggctt gcgcgggaaa ggcgagtgtg gcgctggcca cgacggccag
    11761 cagactcgcg agtcgattct tcatgttgca cctcctcctc gaaatgacgg gtcccctgcg
    11821 atgggggtcc tgcggatcgt ccaatgcttg tggaaggcta gttaatgggg atcatcccct
    11881 tctgtcaagc aagatggcac gcggcccagt gtccgaggcg gatctccctc aacggcggca
    11941 cttccgcact gcagcgagcg accgcgatcg ggcagcgggg atgaaacacg cacccggacg
    12001 gcgggttgag cgggctcggc acctcgccgc gcaccaccat tcgcttgcgc ttggcttcga
    12061 tcttcgggtc gggaatgggc accgccgaga gcagagcctg ggtgtagggg tgtattggct
    12121 cttcgtatag cgcgttgcgg tcggcgagct cggcgatctt gccgaggtac attaccgcca
    12181 cgcgatcgga gatgtggcgc accaccgaca ggtcgtgagc gataaacagg tatgtcagcc
    12241 ccaagcgcga ctgcaactct tcgagcaggt tgatgatctg cgcctggatc gacacgtcaa
    12301 gggccgacac gggctcgtcg caaacgatga gcgcgggctc cgtggcgagg gcgcgcgcga
    12361 tgcccacgcg ctggcgctgc ccccctgaga gctggtgcgg atagcgcctc gcgtgctgcg
    12421 gcagcaggcc cacctggacg aggagctcgc gcacgcgcag ctcccgctgc cccttgtcgg
    12481 gcacgatgcg gtgcaccttg agcggctccg cgaggatctg gcccacggtc atgcgcggat
    12541 tgagcgagga atacgggt
//
LOCUS       DBNHKE010000046         6511 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_112329, whole genome shotgun sequence.
ACCESSION   DBNHKE010000046 DBNHKE010000000
VERSION     DBNHKE010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_112329"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /gene="rplE"
                     /locus_tag="AC16O9_02700"
     CDS             complement(<1..568)
                     /gene="rplE"
                     /locus_tag="AC16O9_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR6515557.1"
                     /translation="MKQMAKDKTAKTEKAEKPGKPAQAQERPAERQAKAPKAEAKPAE
                     KPAKAARPALPVPPARLAIYYREKVVPELVQKYGYKTTMQVPRIRKITLNMGVGESVG
                     DKKTLDNAVADMAKIAGQKPVVTKARRSIANFKLRAGFPIGCMVTLRGARMYEFLDRL
                     VNIAIPRIRDFRGVSNRAFDGRGNYSLGV"
     gene            complement(570..887)
                     /gene="rplX"
                     /locus_tag="AC16O9_02705"
     CDS             complement(570..887)
                     /gene="rplX"
                     /locus_tag="AC16O9_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR6515558.1"
                     /translation="MQRIRKGDEVVVISGRDKGKRGTVLRRVNADYLVVEGVNRVKKH
                     QRPNPMKGVTGGIVDKDMPVHVSNIALFNPATQKADRVGFKELQDGRKVRVFKSNGEQ
                     VDA"
     gene            complement(911..1279)
                     /gene="rplN"
                     /locus_tag="AC16O9_02710"
     CDS             complement(911..1279)
                     /gene="rplN"
                     /locus_tag="AC16O9_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR6515559.1"
                     /translation="MIQMQSILDVADNTGARSVMCIKVLGGSKRRYASIGDVIKVSVK
                     DAAPRGRVKKGEIYDAVVVRTAKGVRRADGSLIRFDSNAAVLLTPKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(1392..1676)
                     /gene="rpsQ"
                     /locus_tag="AC16O9_02715"
     CDS             complement(1392..1676)
                     /gene="rpsQ"
                     /locus_tag="AC16O9_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR6515560.1"
                     /translation="MNESATQAPQAARTRTLVGRVVSDRMQKTVTVLVQRRVKHPVVG
                     KTITLSKKYHAHVEGGEARQGDLVEIAECRPISRTKAWKVTRLIEKSKVV"
     gene            complement(1673..1864)
                     /gene="rpmC"
                     /locus_tag="AC16O9_02720"
     CDS             complement(1673..1864)
                     /gene="rpmC"
                     /locus_tag="AC16O9_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR6515561.1"
                     /translation="MKASELRAKNGDELQKELNDLLKAQFGLRMQVATQQLNNTSQIK
                     KVRRDIARVRTVLKEKAAK"
     gene            complement(1868..2281)
                     /gene="rplP"
                     /locus_tag="AC16O9_02725"
     CDS             complement(1868..2281)
                     /gene="rplP"
                     /locus_tag="AC16O9_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR6515562.1"
                     /translation="MLQPARTKYRKQQKGRNTGIATRGNKVSFGEYGLKAVGRGRLTA
                     RQLEAARRALSRHIKRGGRIWIRVFPDKPISQKPAEVRMGNGKGNPEYFVCEIQPGKI
                     IYEMDGVDAVTAKGAFALASAKLPFKTIVVHRMLG"
     gene            complement(2285..3157)
                     /gene="rpsC"
                     /locus_tag="AC16O9_02730"
     CDS             complement(2285..3157)
                     /gene="rpsC"
                     /locus_tag="AC16O9_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR6515563.1"
                     /translation="MGQKINPIGFRLAVNRNWSSRWYASSRQFAGMLAEDIKVRDFLK
                     KKLAHASVGRVLIERPAKDARITIFSARPGVVIGKKGEEIESLKAELRRLMGVQQVHV
                     NIEEIRKPEIDAQLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNG
                     IEIARTEWYREGRVPLHTLRADIDYGMGEAKTTYGVIGIKVWVYKGEVVAKAETQAVA
                     QPAVVPEPAPAEALAEPKRAKKPVPRPAGRRAPARAKPDGEKKAKKSDAPAPKTTVKR
                     AKKVVKGDGGEKKE"
     gene            complement(3169..3498)
                     /gene="rplV"
                     /locus_tag="AC16O9_02735"
     CDS             complement(3169..3498)
                     /gene="rplV"
                     /locus_tag="AC16O9_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR6515564.1"
                     /translation="METRAILRSARLSAQKGRLVADMIRGQPVDKALNILAFTPKKGA
                     GILKKLLESAIANAEHNDGADIDALKVKSVLVERGSFLKRFHARAKGRGTRVQKHTCN
                     IYVTVGD"
     gene            complement(3641..3895)
                     /gene="rpsS"
                     /locus_tag="AC16O9_02740"
                     /pseudo
     CDS             complement(3641..3895)
                     /gene="rpsS"
                     /locus_tag="AC16O9_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(3909..4736)
                     /gene="rplB"
                     /locus_tag="AC16O9_02745"
     CDS             complement(3909..4736)
                     /gene="rplB"
                     /locus_tag="AC16O9_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR6515565.1"
                     /translation="MPLVKVKPTSPGRRALVKVVTPGLHKGAPHWPLTSSQSKTTGRN
                     NHGRITMRHKGGGHKQHYRMIDFRRNKDGIVAKVERLEYDPNRSAHLALLLYADGERR
                     YVLAPKGVGAGAQLVSGVEAPIKAGNALPLRNIPVGTTVHCIEMLPGKGAQIARSAGA
                     SAQLLAREGSYAQLRLRSGEIRKVHVDCRATIGEVGNEEHNLESIGKAGRKRWRGVRP
                     TVRGVAMNPIDHPHGGGEGRTAAAQPPVSPWGVQTKGYKTRRNKRTQVMIVRDRRTK"
     gene            complement(4740..5054)
                     /gene="rplW"
                     /locus_tag="AC16O9_02750"
     CDS             complement(4740..5054)
                     /gene="rplW"
                     /locus_tag="AC16O9_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR6515566.1"
                     /translation="MTAIKKYPAERLMNVVLAPVVSEKSTLVADKNRQYVFRVADSAT
                     KPEVKAAIELLFKTKVQSVTVSNVKGKEKRFGRFIGRRRSWKKAYVRLAAGQEINFSA
                     TE"
     gene            complement(5051..5668)
                     /gene="rplD"
                     /locus_tag="AC16O9_02755"
     CDS             complement(5051..5668)
                     /gene="rplD"
                     /locus_tag="AC16O9_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR6515567.1"
                     /translation="MELKLISDTGAAGTTSAADAIFGREFNESLVHQIVVAYQANARL
                     GTRKQKERSEIAKSTKKPWRQKGTGRARAGMASSPLWRGGGKIFAASPDENFSHKVNR
                     KMMRAGMTSILSQLAREDRIRVVEELKLEAPKTKLLAQKVKAMGFDEVLVILDEVDPN
                     LELSSRNLPNVAVLTVRQANPVALVRFPHVLFTRKALAKLEEMYK"
     gene            complement(5679..6317)
                     /gene="rplC"
                     /locus_tag="AC16O9_02760"
     CDS             complement(5679..6317)
                     /gene="rplC"
                     /locus_tag="AC16O9_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR6515568.1"
                     /translation="MTIGLVGRKVGMTRIFTDDGGTLPVTVLDVSDNRITQIKTPEKD
                     GYAAVQVAFGKRRANRVRKPLAGHLAKAGVEAGHLLREFRADAKDLANLKVGGKLGVD
                     LFKVGQKVDVQGVSIGKGFAGVIKRHHFSSNRATHGNSISHNKPGSTGQNQDPGRVFP
                     GKRMAGHLGNVVRTIPNLVVVRVDADRQLLLVRGSVPGSKGRDVVVRPAAKA"
     gene            complement(6432..>6511)
                     /gene="rpsJ"
                     /locus_tag="AC16O9_02765"
                     /gene_synonym="nusE"
     CDS             complement(6432..>6511)
                     /gene="rpsJ"
                     /locus_tag="AC16O9_02765"
                     /gene_synonym="nusE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="NusE; involved in assembly of the 30S subunit; in
                     the ribosome, this protein is involved in the binding of
                     tRNA; in Escherichia coli this protein was also found to
                     be involved in transcription antitermination; NusB/S10
                     heterodimers bind boxA sequences in the leader RNA of rrn
                     operons which is required for antitermination; binding of
                     NusB/S10 to boxA nucleates assembly of the antitermination
                     complex; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR6515569.1"
                     /translation="PTDKTVDALMKLDLPAGVDVEIKVQ"
BASE COUNT          858 a         2264 c         2074 g         1315 t
ORIGIN      
        1 gcacgcccaa gctgtagttc ccacggccgt cgaaggcgcg gttcgacacg ccgcggaagt
       61 cgcggatgcg cgggatggcg atgttcacca gccgatcgag gaactcgtac atgcgcgcgc
      121 cgcgcagggt caccatgcag ccgatcggaa agcccgcgcg cagcttgaag ttggcgatcg
      181 aacggcgggc cttggtgacc accggcttct gaccggcgat cttcgccatg tccgcaacgg
      241 cgttgtcgag cgtcttcttg tcgcccaccg actcgcccac gcccatgttg agcgtgatct
      301 tgcggatgcg cggcacctgc atggtggtct tgtagccgta cttttgaacc agctcgggca
      361 ccaccttctc tcggtagtag atcgccaggc gcgccggcgg cacgggcagc gcgggacgcg
      421 ccgccttggc cggcttctcg gcgggcttcg cctcggcctt cggcgccttg gcctgccttt
      481 ctgccgggcg ctcctgcgcc tgggcaggct tgccgggctt ctcggccttt tccgtcttcg
      541 ctgtcttgtc cttcgccatc tgtttcaccc tatgcgtcga cctgttcgcc attcgacttg
      601 aacacccgca ccttgcgccc gtcctgcagc tccttgaagc cgacgcgatc ggccttctgg
      661 gtcgcgggat tgaacagcgc gatgttcgag acgtgcaccg gcatgtcctt gtccacgatg
      721 ccgccggtaa cgcccttcat cggattcggg cgctggtgct tcttgacgcg attcacccct
      781 tccaccacca ggtagtcggc gttgacacgg cgcaggacgg tgccgcgctt gcccttgtcc
      841 cggccggaga tgacgacgac ctcgtcgcct ttgcggatgc gttgcatggc gttcttcctg
      901 tgggggccgc tcagagaacc tcgggggcga gcgacacgat cttcatgaag cgctcggtgc
      961 gcagctcgcg cgtcaccggc ccgaagatgc gggtgccgat cggctccagc ttgggcgtga
     1021 gcagcacggc ggcattgctg tcgaagcgga tcagcgagcc gtcggcgcgg cgcacgccct
     1081 tggccgtgcg caccaccacg gcgtcgtaga tctcgccctt cttcacgcgg ccgcgcggcg
     1141 ccgcatcctt gacgctcacc ttgatcacgt cgccgatgct ggcgtagcgg cgcttggagc
     1201 cgcccagcac cttgatgcac atgaccgagc gcgcacccgt gttgtcggcc acgtccagta
     1261 tcgactgcat ctgaatcatc ggtatttctc catccaactt gcaccacgcg tcgctgcgcg
     1321 caggcagttt tggaccccgt acggggcgaa aaggcgcgga ttctaacgcg aatcccgcgc
     1381 ccagggcaat gctagaccac cttcgacttc tcgatcaggc gcgtcacttt ccatgccttg
     1441 gtgcgcgaaa tggggcggca ctcggcgatc tcgaccaggt ccccctggcg tgcctcgccg
     1501 ccctccacgt gcgcatggta cttcttcgac agcgtgatgg tcttgcccac caccgggtgc
     1561 ttgacccggc gctgcaccag caccgtcacg gtcttctgca tgcggtcgct caccacgcgc
     1621 cccaccaggg tgcgggtgcg ggcggcctgc ggggcctgcg tcgctgactc gttcatttgg
     1681 ctgccttctc cttcagcacg gtgcgcacgc gcgcgatgtc gcggcgcacc ttcttgatct
     1741 ggctggtgtt gttgagctgc tgcgtggcga cctgcatgcg caggccgaac tgggccttga
     1801 gcagatcgtt cagctccttc tgcagctcat caccgttctt ggcgcgaagt tcgctcgcct
     1861 tcatggtcta ccccagcatc ctgtgcacga cgatggtctt gaatggcagc ttggccgagg
     1921 cgagcgcgaa cgcccccttg gcagtgactg catccacccc gtccatttcg tagatgatct
     1981 tgccgggctg gatctcgcac acgaagtact ccgggttgcc cttgccgttg cccatgcgca
     2041 cctcggcggg cttttgcgag atcggcttgt ccgggaacac gcggatccag atgcgcccgc
     2101 cacgcttgat gtggcgtgac agcgcgcgcc gcgcggcctc gagctggcgc gccgtgaggc
     2161 gcccgcggcc gaccgccttc aagccgtact cgccgaacga gaccttgttg ccgcgcgtgg
     2221 cgatgccggt attgcggccc ttttgctgct ttctgtactt ggtgcgtgcc ggttgcagca
     2281 tggcctactc cttcttctcg ccgccgtcgc ccttgacgac cttcttggca cgtttcaccg
     2341 tggtcttggg ggccggggcg tcggacttct tcgccttctt ctcgccgtcc ggcttggcgc
     2401 gcgccggcgc gcgccggcct gccggccgcg gtacgggctt cttcgcgcgc ttgggctccg
     2461 ccagcgcctc ggccggcgcc ggctccggca cgacggcggg ctgagctacc gcttgcgtct
     2521 cggccttggc caccacctcg cccttgtata cccagacctt gatgccgatc acgccatagg
     2581 tagtcttcgc ctcgcccatg ccgtagtcga tgtcggcgcg cagcgtatgc agcggcacgc
     2641 ggccctcgcg gtaccactcg gtacgcgcga tctcgatgcc gttcaagcgc cccgcgctca
     2701 tgatcttgat gccttgagcc ccgaggcgca tggcgttctg catcgcgcgc ttcatggcgc
     2761 gacggaacat gatgcgcttt tcaagctgct gggcgatgga atcggcgatc agctgcgcgt
     2821 cgatttcggg cttcctgatc tcctcgatgt tgacgtgcac ctgctgcacg cccatcaggc
     2881 gccgcagctc cgccttgagc gattcgatct cctcgccttt cttgccgatg accacgccgg
     2941 gccgggcgga aaagatggtg atgcgcgcat ccttcgccgg gcgctcgatg agcacgcgcc
     3001 cgacggacgc gtgcgcgagc ttcttcttga ggaaatcgcg aaccttgatg tcctcggcca
     3061 gcatgcccgc aaactggcgg ctgctcgcat accagcgcga gctccagttg cggttgaccg
     3121 ccagccggaa gccgatcgga ttgatcttct gtcccatgtg cgtggtccct agtcgccgac
     3181 cgtgacgtaa atgttgcagg tgtgcttctg cacgcgtgtg ccgcgcccct tggcgcgcgc
     3241 gtggaagcgc ttgaggaacg agccgcgctc gacgagcacg ctcttcacct tcagggcgtc
     3301 gatgtcggcg ccgtcgttgt gctcggcgtt ggcgatcgcc gactcgagca gcttcttcag
     3361 gatgcccgcg cccttcttcg gcgtgaaggc gaggatgttg agcgccttgt ccacgggctg
     3421 gccgcggatc atgtccgcga ccaggcggcc cttctgggcc gagaggcgcg cgctgcgcag
     3481 gatggctcgc gtttccatga gtgcccctct acttcttcgc cgccggtgcc gatgacgaag
     3541 ccggggccgc ggccggcgtt gccgccgccg gcgctgccgc cccgggcgcg cccaccggct
     3601 tgcgctcgct gcccaccgcc tccgcggtgc gcttgccaac cgcagagtgg ctcttgaaca
     3661 tgcgcgtgag cgcaaactcg ccgagcttgt ggcccaccat gttttcggtg atgtacaccg
     3721 ggatgtgctg cttgccgttg tgcaccgcca ccgtcagccc gatgaactcc ggcatcacgg
     3781 tcgaacggcg cgaccaggtc ttgatcggac gcttgtcgtt gccggcgcgc gccgccgcga
     3841 ccttgtccat cagatgccgg tctacgaacg ggcccttctt gattgagcgt gccatatctg
     3901 tctagccttt atttcgtgcg ccggtcgcgc acgatcatga cttgtgtgcg cttgttgcgg
     3961 cgagtcttgt agcccttggt ctgcacgccc cagggcgaca ctggaggctg ggctgccgcg
     4021 gtgcggccct cgccgccacc gtgcggatgg tcgatcggat tcatggccac gccgcgcacc
     4081 gtcgggcgca cgccgcgcca gcgcttgcgg ccggccttgc cgatggactc gaggttatgc
     4141 tcttcgttgc cgacttcgcc gatagtggcg cggcagtcaa cgtgaacctt gcggatctcg
     4201 cccgagcgca gccgcagctg cgcgtaggaa ccctcgcgcg ccagaagctg cgccgaggcg
     4261 cctgcagagc gcgcgatctg cgcccccttg cccgggagca tctcgatgca gtgcaccgtc
     4321 gtgcccaccg gaatgttgcg caggggcagc gcgttgccgg ccttgatcgg cgcctctacc
     4381 ccggacacca gctgcgcacc agcgccgacg cccttcggcg ccagcacgta gcgccgctcc
     4441 ccgtcggcgt agagcagcag cgcgagatgc gcgctgcgat tcgggtcgta ctcgagccgc
     4501 tccacctttg ccacgatgcc gtccttgttg cggcggaagt cgatcatgcg gtagtgctgc
     4561 ttgtggccgc cacccttgtg gcgcatggtg atgcggccat ggttgttgcg gccggtcgtc
     4621 ttcgactggc tcgaggtgag cggccagtgc ggcgcgccct tgtgcaggcc gggcgtcacc
     4681 accttgacca gcgcgcggcg gccgggcgaa gtgggtttga ctttgaccaa tggcatggct
     4741 tactccgtcg ccgagaagtt gatttcctgc ccggcagcca ggcgcacgta ggccttcttc
     4801 cagctgcgac ggcgcccgat gaagcgcccg aagcgctttt ccttgccctt cacattggat
     4861 accgtgaccg actgcacctt ggtcttgaac agaagctcga tcgccgcctt gacctcgggc
     4921 ttggtggcgc tgtcggcgac gcggaacaca tactggcgat tcttgtccgc gaccagcgtg
     4981 ctcttctccg acaccaccgg cgccagcacg acgttcatga gccgctcggc ggggtacttc
     5041 ttgatcgccg tcatttgtac atctcctcga gcttcgccag cgctttgcgc gtgaacagca
     5101 cgtgcggaaa gcgcaccagc gccaccgggt tcgcctggcg caccgtcagc acggcgacgt
     5161 tcggcaggtt gcgcgacgac agctccagat tcgggtcgac ttcgtccagg attaccagca
     5221 cctcgtcgaa gcccattgcc ttcaccttct gcgcgagaag cttggtcttc ggcgcctcga
     5281 gcttgagctc ttccacgacg cggatgcggt cctcgcgcgc gagctgcgac aggatcgagg
     5341 tcatgccggc gcgcatcatc ttgcggttga ccttgtgcga gaagttctcg tccgggctcg
     5401 cggcgaagat cttgccgccg ccgcgccaca gcgggctcga ggccatgccg gcgcgcgcgc
     5461 gccccgtgcc cttctgccgc cacggcttct tcgtcgactt ggcgatctcg ctgcgttcct
     5521 tttgcttcct cgtgcccagg cgcgcgttcg cctggtaggc gacgacgatc tggtgcacga
     5581 gcgattcgtt gaactcgcgc ccgaagatgg catccgccgc gctggtggtg cccgcggcgc
     5641 ccgtgtcgct gatcagtttc agttccatcg cgcccccgct acgccttcgc cgcgggccgc
     5701 acgacgacgt cgcggccctt ggatcccggt accgagcccc ggaccagcag cagctgccgg
     5761 tcggcgtcga cacgcacgac caccagattg gggatggtgc gcactacgtt gccgagatgc
     5821 cccgccatgc gctttccggg aaacacgcgg cccgggtcct ggttctggcc cgtggacccc
     5881 ggcttgttgt gcgagatgga gttgccgtgc gtggcgcggt tggaggagaa gtggtggcgc
     5941 ttgatcacgc cggcgaagcc cttgccgatg gacacgccct gcacgtccac cttctgtccg
     6001 accttgaaca gatcgacgcc cagcttgccg ccgaccttca gattggcgag gtccttggcg
     6061 tcggcgcgga attcccgcag caggtggccg gcttcgacgc cggccttggc gaggtgcccg
     6121 gcgaggggct ttctgacgcg gttggcgcgg cgcttgccga acgccacctg tacggcggcg
     6181 tagccgtcct tctccggcgt cttgatctgc gtgatgcggt tgtcggagac gtcgagcacc
     6241 gtcaccggca gcgtgccgcc gtcgtcggta aagatgcgcg tcatgccgac cttacggccg
     6301 accagtccaa tcgtcatacc gtcctcttct gtcttgaccc gatgccgctt gccgcggccc
     6361 cgggcgccgg ctgcaattgg ccggcccttc acctcgtact gcgtgcgtcc cgcagcgctt
     6421 cgcgctgccc gttactgcac cttgatctcc acgtccacgc ccgccggcag atcgagcttc
     6481 atcagcgcgt ccactgtctt gtcggtcggg t
//
LOCUS       DBNHKE010000047         1889 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_641339, whole genome shotgun sequence.
ACCESSION   DBNHKE010000047 DBNHKE010000000
VERSION     DBNHKE010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_641339"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="AC16O9_02770"
     CDS             <1..686
                     /locus_tag="AC16O9_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR6515570.1"
                     /translation="PPLPEGRSGFLDEFESLALLAQAGLAVVEHRLCRSETEARAAYA
                     TLGPKVVVKACSASVPHKTEQGLVRVGLQSEDAVAQAFRDFAPRLPAGDGVLVARHAA
                     GQRELALGARLDPLFGPVVMVGDGGIYLEALKDFRLLLPPFTQDDVLAALAGLRLAPL
                     LGAQRGQAPLDAGAYARMAVRLGEAMRAWRGRIASVDVNPVMLFEQGRGALAVDALVE
                     MAPACANPG"
     gene            737..1825
                     /locus_tag="AC16O9_02775"
     CDS             737..1825
                     /locus_tag="AC16O9_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6515571.1"
                     /translation="MKRRKFLQGAAAGGVAAAAATVSAPAISQGIKQLKMVTTWPKNF
                     PGLGTGAERLAARINTMSGGKLEVKVYAAGELVPAFESFNAVRDGKAEMSHSVSYYWQ
                     GQSPAFNFFAAVPFGFNAAEHAGWMLWGDGHKLWSELCDQFNIVPFMRASTGVQMGGW
                     YRKEMKTMDDFKGLKFRMPGLGGEVLRKLGGTVVNLPGGEIFPALQAGTIDGTEWVGP
                     WNDLAFGFYKVAKFYYWPGFHEPCTTGEVLVNKKVWQGLSKDLQEIIAVAVQAEATIE
                     LAEFNYKSSDALDTLLTKHNVQLRKFSDDMLKKIGEAAIEVVAEIGNKDPFTKRVYES
                     FLAARKRAIGWSALGEQGYMNARSLIKF"
BASE COUNT          314 a          579 c          678 g          318 t
ORIGIN      
        1 ccccgccgct gcccgagggc aggtcgggct ttctcgacga gttcgagagc ctcgcgctgc
       61 tggcgcaggc gggcctggcc gtggtcgagc accggctgtg ccgcagcgag acggaggcgc
      121 gcgccgctta cgccacgctc gggccgaagg tggtggtcaa ggcctgctcg gcgagcgtgc
      181 cgcacaagac cgagcagggg ttggtgcgcg tgggcctgca aagcgaggat gcggtggcgc
      241 aagccttccg cgacttcgcg ccgcggctgc cggcgggcga cggggttctg gtcgcaaggc
      301 acgccgcggg ccagcgcgag ctggcgctcg gcgcgcggct cgatcccctt ttcggtccgg
      361 tggtcatggt cggcgacggc ggcatctatt tggaagcgtt gaaggatttc cgccttttgc
      421 tgccgccgtt cacgcaggac gacgtgctgg cagcgctcgc cggactgcgc ttggcgcccc
      481 tgctcggggc gcagcgcggc caggcgccgc tcgatgccgg tgcatacgcg cgcatggcag
      541 tgcgcctggg cgaggcgatg cgcgcctggc ggggccgcat tgcctcggtg gacgtcaacc
      601 cggtgatgct gttcgagcag gggcgaggcg cgctcgcggt cgatgcgctc gtcgaaatgg
      661 ccccggcttg cgcgaatccg gggtaacttg gatactcgta cccgttttca gtcgaactac
      721 ggaggaggga gggttcatga agcgtcgtaa attcctacaa ggcgcagcgg cgggcggcgt
      781 cgccgcagcg gccgccacgg tatccgcgcc ggcaatttcg cagggcatca agcagctgaa
      841 gatggtgacc acctggccga agaactttcc cggcctgggc acgggcgccg agcgcctggc
      901 cgcgcgcatc aatacgatgt cgggcggaaa gctcgaggtg aaggtgtacg cggccggcga
      961 gctggtaccc gcgttcgagt ctttcaacgc ggtgcgcgac ggcaaggccg agatgagcca
     1021 ttctgtctcg tactactggc agggccaatc gccggcgttc aatttcttcg ccgcggtgcc
     1081 cttcggcttt aatgccgccg agcacgccgg gtggatgctg tggggcgacg gtcacaagct
     1141 gtggagcgag ctgtgcgacc agttcaacat cgtcccgttc atgcgcgcct ccaccggcgt
     1201 gcagatgggc ggctggtacc gcaaggaaat gaagacgatg gacgacttta agggcctcaa
     1261 gttccgcatg ccgggactgg gcggcgaagt gctgcgcaag ctaggcggga cggtggtcaa
     1321 cctgccgggc ggcgagattt ttcccgcgct gcaggccggc accattgacg gcacggaatg
     1381 ggtcggcccc tggaacgacc tcgccttcgg cttctacaag gtcgccaagt tctactactg
     1441 gccgggcttc cacgagccct gcaccacggg cgaggtgctg gtcaacaaga aggtgtggca
     1501 gggcctgtcc aaggacttgc aagaaatcat tgcagtggcg gtgcaggcgg aggcgacgat
     1561 cgagctcgcc gagttcaact acaagagttc ggacgcgctg gacacgctgc tcaccaagca
     1621 caatgtgcag ttgcgcaagt tttccgacga catgctgaag aagatcggcg aggccgcgat
     1681 cgaggtggtg gccgagatcg gcaacaagga tcccttcacc aagcgcgtgt acgagagctt
     1741 cctcgccgcg cgcaagaggg cgatcggctg gagcgcgctc ggcgagcagg gctacatgaa
     1801 cgcccgttcg ctgatcaagt tctagacgtc tgtcgcgagg taactacagc ctaggcgcgg
     1861 gcaacccccg cgcctaggtt cttctgcac
//
LOCUS       DBNHKE010000048         7148 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1329133, whole genome shotgun sequence.
ACCESSION   DBNHKE010000048 DBNHKE010000000
VERSION     DBNHKE010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1329133"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="AC16O9_02780"
     CDS             <1..1592
                     /locus_tag="AC16O9_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR6515572.1"
                     /translation="ETVDGEFQTYAATDGRFNREHFFNKYPELKALVADLTDDDIDRL
                     KRGGHDPVKIYSAYHAAVNHKGQPTVILAKTKKGYGMGTWGQGKMGTHQQKKLDDDAL
                     KAFRDRFALPLSDEDVTGLKFYRPADGSPEMRYLHAKRERLGGYLPERKTEGKKLKAP
                     ARESFKRFLDGSGGREQSTTMQYVQLLTQLLRDPGLGRHIVPIVADEARTFGMQSLFR
                     NVAIYAPHGQLYEPEDREELLYYKEAQDGQILEEGITEAGALASWIAAATSYSTHGVP
                     MLPFYIFYSMFGFQRVGDLIWAAADSRSRGFLVGATAGRTTLAGEGLQHQDGSSHFVA
                     STVPNCVAYDPCYGYELAVILQDGARRMLEADEDVFYYVTVMNENYAHPAMPAGAEEG
                     ILKGMHRVRDAGKGKRHVRLLGAGAILREALAAAETLESEHGVAAEVWSVTSFTELRR
                     EGRSGGQSWVAQCLAGGKGPVIAASDYVSGLADLIREWVPGKYVTLGTDGFGRSDTRA
                     ALRRHFGVDRDSIVRAALGAL"
     gene            1594..2748
                     /locus_tag="AC16O9_02785"
     CDS             1594..2748
                     /locus_tag="AC16O9_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6515573.1"
                     /translation="MIPRTLFSPEHETFRDSVRRFMQTEVVPHDERWQEQGYVDREVW
                     KKAGANGFLCCSMPEEFGGAGADRLYSMVLMEEQARANNSSLGWGLHSEIVAPYLLRY
                     GSEALKKAYLPKMAAGEMIGAIAMSEPGAGSDLQAVKTTAVKKGGAYLVNGSKTFITN
                     GWHCDLVIVVAKTDPSKGAKGTSLVVVDTSMKGFTKGKRLKKLGLKGQDTAELFFDNV
                     EVPAQNLLGQENNGFIYLMQELPWERMQIAIAAVAKAEAAVEWTKAYVTERKAFGRTV
                     ASFQNTRFKLAELATEVQVARVFVDRCMELLLAEKLDTATASMAKYWVTDLECRVIDE
                     CLQLHGGYGFMWEFPIARAYADARVQRIYGGTNEIMKEVISRSLGLGGDR"
     gene            2813..4030
                     /locus_tag="AC16O9_02790"
     CDS             2813..4030
                     /locus_tag="AC16O9_02790"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6515574.1"
                     /translation="MAKSANEAMIFDAIRTPRGKGKRDGSLHEVKPVTLLAGVLQELQ
                     RRNDFDTGEVEDVVMGCVTPSGEQGSCIAKTAALAAGWDWKVPGFQLNRFCASGLEAV
                     NLAAQKVRSGWEDLVVAGGIESMSRVPMGSDRGAWACDPQTSIDTAFIPQGISADLIA
                     TLEEFSRDEVDEFALKSQKKAAAARESGKFKKSIVPVKDALGDLILGEDEFIKPHTTK
                     EGLAALKLSFDKVGEMGYDAVAIRRYPMVERIRHVHTAGNSSGIVDGSAAVLIGTEKK
                     GKSLGLKPRARIVAAALSGADPTIMLTGPMPAARKALEKAKMKIERIDLFEVNEAFAV
                     VPMKFMKEMGVPEEKVNVNGGSIAMGHPLGATGAMILGTLIDELERRKLRYGLATLCV
                     GGGMGIATIVERL"
     gene            4031..6142
                     /locus_tag="AC16O9_02795"
     CDS             4031..6142
                     /locus_tag="AC16O9_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR6515575.1"
                     /translation="MAVRYEKGGDNVVTLTLDAPGAPVNTMTAAWEKAFDEATARLEK
                     EKDGIAGVILCSAKKTFFAGAELKDVLKLRPEDGAKAFAWLESLKLRLRRLERLGKPV
                     VAAIEGAALGGGWELALAAHCRICLNDERIELGLPEVTLGLLPGAGGIVKTVRLVGLQ
                     AALPLLLEGKNMRPAQAAKLGFLQGLAANRDDLFRMAREWIAANPAPKQPWDDESYRI
                     PGGTPASPALAQMLTIAPAMLIEKTRGLYPAPEAIMAAATEGASVDFDTAMRIESRYG
                     AKLIVSPVAKNMITLFFNRTAIRSGASRPKDVPKWKATKVGILGAGMMGGGIAWANAS
                     RGVPCVLKDVTLEQALKGKAYGEKLAAKRNRPQIPELIKPTANAADLQGCDLIIEAVF
                     EKRELKAQVTKEAEPMLAAGGFFASNTSTLPITGLAQAFSAPERFVGLHFFSPVDRMQ
                     LVEIIKGKQTSAETLARAYDYVLQIGKIPIVVNDARGFYTSRTFGTFVAEGCSMLTEG
                     IPAAVIENAGRFAGMPVGPLAVIDETSMSLSVSVMDQAKADLEAEGRKYTPLPGQEVI
                     QRMVKEFKRPGRAGGGGFYEYPKEGKKYLWPELAKIFGKKNAKWDFEELKERILYRQA
                     IETARCLEQGVLEHVHDGNIGSVFGIGFPAWAGGALQYVNHIGIKQFVKRADQLARKI
                     GPRFAPPRLLRELAAKGKPLA"
     gene            6142..7086
                     /locus_tag="AC16O9_02800"
     CDS             6142..7086
                     /locus_tag="AC16O9_02800"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6515576.1"
                     /translation="MALPPILQRLKLPVVGAPMFIAGNPKLVIEQCRAGIVGSFPALN
                     ARPKEALDQWLTEIEQALAGAPDAAPYAVNQIVHKSNDRLEHDLEVCIRHKVPITITS
                     LRAPNDVIPAIHAYGGIVLHDVINVRHAEKALEAGVDGLILVCAGAGGHAGMLSPFAL
                     VGEVRRFFDGPIILSGAIATGDAILAALASGADLAYIGTRFLATPEASVPERYKQEIL
                     KASAADIVYTDLFSGVNGNYLKHSVLAAGFDPLNLPKSDPKKMNFGSASGAEKKVWRD
                     IWSAGQGVGQIDAVQPVTEVVAKLAAEYTAARRRLALS"
BASE COUNT         1264 a         2380 c         2481 g         1023 t
ORIGIN      
        1 acgagacggt cgatggcgaa ttccagacct atgccgccac cgacgggcgc ttcaaccgag
       61 agcacttctt caacaagtac ccggagctga aggcgctggt ggccgacctg accgacgacg
      121 acatcgatcg tttgaagcgc ggcggccacg acccggtgaa gatctactcc gcgtaccacg
      181 cggcggttaa ccacaagggc cagcccacgg tgatcctcgc caagaccaag aagggctacg
      241 gcatgggcac ctggggccag ggcaagatgg gcacccacca gcagaagaag ctcgacgacg
      301 acgccctgaa ggcgttccgc gaccgcttcg cgctgccgct ctcggacgag gacgtgaccg
      361 gcctcaagtt ctaccggccg gcggacggct cgcccgagat gcgctacctg cacgcgaaac
      421 gcgagcgcct cggcggctac ctgccggagc gaaagaccga aggcaagaag ctgaaggcgc
      481 ccgcgcgcga gtcgttcaaa cggttcctcg acggctccgg cgggcgcgag cagtccacca
      541 ccatgcagta cgtgcagctc ctgacgcagc tgctcaggga cccgggcctc ggccggcaca
      601 tcgtgccgat cgtcgccgac gaggcgcgca ccttcggcat gcagtcgctg ttccgcaatg
      661 tcgcgatcta cgcgccgcac gggcagctgt acgagccgga ggaccgcgag gagctgctct
      721 attacaagga agcgcaggac ggccagatcc tggaggaggg catcaccgag gcgggcgcgc
      781 ttgcttcgtg gatcgcggcg gccaccagct acagcacgca cggcgtgccg atgctgccgt
      841 tctacatctt ctattcgatg ttcggcttcc agcgcgtcgg cgacctgatc tgggcggcgg
      901 ccgactcgcg ctcgcgcggc tttctcgtcg gcgccacggc gggccgcacc acgctcgcgg
      961 gcgaagggct gcagcaccag gacggctcga gccatttcgt cgcgtcgacc gtcccgaact
     1021 gcgtcgccta cgacccctgc tacggctacg agctggccgt catcctgcag gacggcgcgc
     1081 gccgcatgct cgaggcggac gaggacgtct tctattacgt cacggtgatg aacgagaact
     1141 acgcgcaccc ggcaatgccg gcgggcgcgg aggaagggat cctgaagggc atgcaccggg
     1201 tgcgcgacgc gggcaagggc aagcgccacg tcaggctgct gggcgcgggc gcgatcctgc
     1261 gcgaggcgct ggccgcggcc gagacgctcg agtcggagca cggcgtcgcc gctgaagtgt
     1321 ggtcggtgac gagcttcacc gagctgcggc gcgagggccg ttcaggcggg caaagctggg
     1381 tagcgcagtg cctcgcgggc ggcaagggcc cggtgatcgc ggcgagcgac tacgtgagcg
     1441 gcctcgccga cctgatccgc gagtgggtac cgggcaagta cgtcacgctc ggcaccgacg
     1501 gcttcggccg cagcgacacc cgcgccgcgc tgcgccggca cttcggcgtg gatcgcgaca
     1561 gcatcgtgcg cgcggcgctc ggcgcgctgt agcatgatcc cgcgcacgct gttctcgccc
     1621 gagcacgaga ccttccgcga cagcgtgcgc cgcttcatgc agaccgaggt ggtgccgcac
     1681 gacgagcgct ggcaggagca gggctacgtc gaccgcgagg tgtggaagaa ggcgggtgcc
     1741 aacggctttc tgtgctgctc gatgcccgaa gagttcggcg gcgcgggcgc cgaccggctc
     1801 tacagcatgg tgctgatgga ggagcaggcg cgcgccaaca attcgtccct cggctggggg
     1861 ctgcactccg agatcgtcgc gccgtacctc ctgcgctacg gcagcgaagc gctgaagaaa
     1921 gcgtacctgc cgaagatggc cgccggcgag atgatcggcg ccatcgccat gagcgagccc
     1981 ggcgcgggct ccgacctgca ggcggtgaag accaccgcgg tgaagaaggg cggcgcgtac
     2041 ctcgtcaatg gctcgaagac cttcatcacc aacggctggc attgcgacct cgtcatcgtc
     2101 gtggcgaaga ccgacccgtc aaagggcgcc aagggcacga gcctcgtcgt cgtcgacacg
     2161 tcgatgaagg gcttcaccaa gggcaagcgc ctgaagaagc tggggttgaa gggccaggac
     2221 accgccgagc tcttcttcga caacgtcgag gtcccggcgc agaacctgct cgggcaggag
     2281 aacaacggct tcatctacct catgcaggag ctgccctggg agcgcatgca gatcgcgatc
     2341 gcggcggtgg ccaaagccga ggccgcggtg gagtggacca aggcgtatgt caccgagcgc
     2401 aaggccttcg gcaggacggt ggcgagcttc cagaacacgc gcttcaagct cgccgagctc
     2461 gccaccgagg tgcaggtggc gcgcgtcttc gtcgaccgct gcatggagct gctgctcgcc
     2521 gaaaagctcg acacggccac cgcctcgatg gcgaagtact gggtcacgga cctcgagtgc
     2581 cgggtgatcg acgaatgcct gcagctgcac ggcggctacg gcttcatgtg ggagttcccc
     2641 atcgcgcgcg cctacgcgga cgcgcgcgtg cagcgcatct acggcggcac caacgagatc
     2701 atgaaggaag tgatttcacg ctcgctcggc ttgggcggtg atagataggg acagtcccca
     2761 tttacgcttc tgcttttcgt aaatggggac tgtccctctt acggaggttg cgatggccaa
     2821 gagtgcaaac gaagcgatga ttttcgacgc gatccgcacc ccgcgcggca agggcaagcg
     2881 cgacggctcg ctgcacgagg tgaagcccgt gacgctgctc gcgggcgtgc tgcaggagct
     2941 gcagcgccgc aacgacttcg acacgggcga ggtcgaggac gtggtgatgg gctgcgtcac
     3001 gccctcgggc gagcagggct cgtgcatcgc caagaccgcg gcgctcgccg cgggctggga
     3061 ctggaaggtg ccgggcttcc agttgaaccg cttctgcgcc tcgggcctcg aggcggtcaa
     3121 cctcgcggcg cagaaggtgc gctcgggctg ggaggacctg gtcgtcgccg gcgggatcga
     3181 gtccatgtcg cgcgtgccaa tgggctcgga ccgcggcgcc tgggcctgcg acccgcaaac
     3241 gagcatcgac acggcgttca tcccgcaggg catcagcgcc gaccttatcg ccacgctgga
     3301 ggaattttcg cgcgacgagg tggacgagtt cgcgctgaag tcccagaaga aggccgccgc
     3361 cgcgcgcgag agcggcaagt tcaagaagtc gatcgtgccg gtgaaggacg cgctcggcga
     3421 cctgatcctc ggcgaagacg agttcatcaa gccgcacacg acgaaggaag gcctcgccgc
     3481 gctgaagctg tccttcgaca aggtgggcga gatgggctac gacgcggtgg cgatccgccg
     3541 ctatccgatg gtcgagcgca tccgccacgt gcacaccgcg ggcaactcct ccgggatcgt
     3601 cgacggctcg gccgcggtgc tcatcggcac cgagaagaag ggcaagagcc tgggcctgaa
     3661 gccgcgcgcg cgcatcgtcg cggcggcgct ctcgggtgcc gaccccacca tcatgctcac
     3721 cggcccgatg cccgcggcgc ggaaggccct cgaaaaggcg aagatgaaga tcgagagaat
     3781 cgacctgttc gaagtgaacg aggccttcgc cgtggtgccg atgaagttca tgaaggagat
     3841 gggcgttccc gaggagaagg tgaacgtgaa cggcggctcg atcgccatgg gccacccgct
     3901 cggcgcgacc ggggccatga tcctcggtac gctcatcgac gagctcgagc gccgcaagct
     3961 ccgctacggc ctcgccacgc tgtgcgtggg cggcggcatg ggcatcgcca ccatcgtcga
     4021 gcgcctgtag atggcagtcc gctacgaaaa gggaggcgac aacgtcgtca ccctgaccct
     4081 cgacgcaccc ggagcgccgg tcaacaccat gaccgcggcc tgggagaagg cattcgacga
     4141 agccaccgcg cggctggaaa aggaaaagga cggcattgcc ggcgtaatcc tgtgctcggc
     4201 gaagaagacc ttcttcgccg gtgccgagct gaaggacgtc ttgaagctca ggcccgagga
     4261 cggcgcgaag gcctttgcct ggctcgaatc gctcaagttg cggctgcggc ggctcgagcg
     4321 gttgggcaag cccgtggtgg cggcgatcga gggcgcggcg ctcggcggcg gctgggagct
     4381 tgcgctcgcc gcccactgcc gcatctgcct gaacgacgag cgcatcgagc tcggcctgcc
     4441 cgaagtgacg ctgggcctcc tgcccggcgc gggcggcatc gtgaagacgg tgcgcctggt
     4501 cggcctgcag gcggcgctgc cgctgctgct cgaaggcaag aacatgcgtc ccgcgcaagc
     4561 cgcgaagctc ggcttcctgc aggggcttgc cgcgaaccgc gacgatctgt ttcgcatggc
     4621 gcgcgagtgg atcgcggcca accccgcgcc caagcagccc tgggacgacg agagctaccg
     4681 catccccggc ggcacgcccg cgagcccggc gctcgcgcag atgctcacca tcgcgccggc
     4741 gatgctgatc gagaagacgc gcggcctgta cccggcgccc gaagccatca tggcggcggc
     4801 gaccgaaggc gcgagcgtgg acttcgacac ggcgatgcgc atcgagtcgc gctacggcgc
     4861 caagctcatc gtcagcccgg tggcgaagaa catgatcacg ctgttcttca accgcacggc
     4921 gatccgctcg ggtgcctcgc ggccgaagga cgtgcccaag tggaaggcga cgaaggtcgg
     4981 catcctcggt gcgggcatga tgggcggcgg catcgcctgg gcgaacgcga gccgcggcgt
     5041 gccctgcgtg ctgaaggacg tgaccctcga gcaggcgctc aagggaaagg cgtacggcga
     5101 gaagctggcc gccaagcgca accggccgca gattcccgag ctcatcaagc cgacggccaa
     5161 cgcagcggac ctgcagggct gcgacctgat catcgaggcg gtgttcgaga agcgcgagct
     5221 caaggcgcag gtgaccaaag aagccgagcc gatgctcgcc gcgggcggct tcttcgcgtc
     5281 caacacgtcc acgctgccga tcacgggcct tgcgcaggcg ttctcggcgc ccgagcgctt
     5341 cgtcggcctg cacttcttct cgcccgtcga tcgcatgcag ctcgtcgaga tcatcaaggg
     5401 caagcagacc tccgcggaga cgctggcgcg cgcctacgac tacgtgctgc agatcggcaa
     5461 gatcccgatc gtggtgaacg acgcgcgcgg cttttacacc agccgcacct tcggcacctt
     5521 cgtcgccgag ggctgctcga tgctcaccga aggcattccc gcggcggtga tcgaaaacgc
     5581 cgggcgcttc gccggcatgc cggtgggccc gctcgcggtg atcgacgaga cctcgatgtc
     5641 gctctcggtc tccgtcatgg accaggccaa ggcggacctc gaggccgaag gcaggaaata
     5701 cacgccgctc cccgggcagg aagtgatcca gcgcatggtg aaggagttca agcgccccgg
     5761 gcgcgcgggc gggggcggct tctacgagta tccgaaagag ggcaagaagt acctctggcc
     5821 ggagcttgca aagatcttcg gcaagaagaa cgccaagtgg gacttcgagg agctgaagga
     5881 gcgcatcctc taccggcagg cgatcgagac cgcgcgctgc ctcgagcaag gcgtgctcga
     5941 gcacgtgcac gatggcaaca tcggctcggt gttcggcatc ggctttccgg cctgggccgg
     6001 cggcgcgctg cagtacgtga accacatcgg catcaagcag ttcgtcaagc gcgccgacca
     6061 gctcgccagg aagatcggcc cgcgcttcgc cccgccgagg ctcctgcgcg agctcgccgc
     6121 aaagggcaag cccctcgcct gatggcgctg ccgccgatcc tgcagcgcct gaagcttccc
     6181 gtcgtcggcg cgccgatgtt catcgccggc aacccgaagc tcgtcatcga gcagtgccgg
     6241 gccggcatcg tcggctcgtt tcccgccctc aacgcgcgcc cgaaggaggc gctcgatcag
     6301 tggctgaccg aaatcgagca ggcgctcgcg ggcgcgccgg acgccgcccc ctacgcggtg
     6361 aaccagatcg tgcacaagtc gaacgaccgc ctcgagcacg acctcgaggt gtgcatccgc
     6421 cacaaggtgc cgatcacgat cacgagcctc agggcgccga acgacgtcat ccccgcgatc
     6481 cacgcctacg gcggcatcgt gctgcacgac gtgatcaacg tgcgccacgc cgaaaaagcg
     6541 ctcgaggcgg gcgtcgacgg gctgatcctg gtctgcgcgg gcgcgggcgg ccacgccggg
     6601 atgctctcgc cgttcgcgct ggtgggcgag gtgcggcgct tcttcgacgg ccccatcatc
     6661 ctctcgggcg ccattgccac gggcgacgcg atccttgccg cgctcgcgtc gggcgcggac
     6721 ctcgcctaca tcggcacgcg ctttctcgcc acgccggaag cgagcgtgcc cgagcgctac
     6781 aagcaggaga tcctcaaggc gagcgctgcc gacatcgtct ataccgacct cttctcgggc
     6841 gtgaacggca actacctcaa gcacagcgtg ctcgcggccg gcttcgatcc gctgaacctg
     6901 cccaagtccg atccgaagaa gatgaatttc ggctccgcga gcggtgccga gaagaaggtc
     6961 tggcgcgaca tctggagcgc ggggcagggc gtcgggcaga tcgacgccgt gcagccggtg
     7021 accgaggtgg tggcgaagct cgccgccgag tacacggcgg cgcggcgccg gctggccctg
     7081 tcgtgatcct cgacatccag ccgcgcggcc ccgccttctg ctcgggcggc gacctcgcca
     7141 agatggag
//
LOCUS       DBNHKE010000049        17108 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1276219, whole genome shotgun sequence.
ACCESSION   DBNHKE010000049 DBNHKE010000000
VERSION     DBNHKE010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1276219"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..737
                     /locus_tag="AC16O9_02805"
     CDS             120..737
                     /locus_tag="AC16O9_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6515577.1"
                     /translation="MTRRLAGAALALAAGFALANPLAPDALVKDVMLDIQRIIVADKD
                     LKEGDRAKTTRLIEDKALPHFNHVGMTALAVGASWRKATPEQKQRLAEEFRTLLVRTY
                     ASSLSAFGDQKFEFRPLRMKPEDTDVTVEVRVLQPGSQPVVVEYDMEKNARGWQVYDV
                     RVGGISLVVNYRTEFGNIVRDKGIDGLIGTLATKNKSLAAADTKK"
     gene            734..1024
                     /locus_tag="AC16O9_02810"
     CDS             734..1024
                     /locus_tag="AC16O9_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6515578.1"
                     /translation="MIRVDGARLYVSGPATLATAAQLLEAARAPLAAGVRSIDLGEVT
                     ELDSSLLAVLFEWMREARAGGQALVLERLPADLKSLAQLYGVAELLPQDAAS"
     gene            1021..1752
                     /locus_tag="AC16O9_02815"
     CDS             1021..1752
                     /locus_tag="AC16O9_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6515579.1"
                     /translation="MTPAIEVRGVEKRYGALQALAGVTLEVRQGEFFGLLGPNGAGKT
                     TLINIIAGLARASAGSVAVLGADVVADYRRARAMLGVVPQELVFDPFFTVRETLRFQS
                     GYFGIRANDAWIDEVMHHLDLDAKAEANMRSLSGGMKRRVLVAQALVHKPPVIVLDEP
                     TAGVDVELRQGLWQFVRRLNRDGHTIVLTTHYLEEAEEHCQRIAMLKAGRIVALDTTQ
                     NLLASFAGPHPADLEDVFLHLMQKH"
     gene            1756..2511
                     /locus_tag="AC16O9_02820"
     CDS             1756..2511
                     /locus_tag="AC16O9_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6515580.1"
                     /translation="MTGFVTLLYKELLRFWKVAYQTVGGPVLSALLYVVIFSQALGGH
                     VRVFEGVPYTAFLVPGLVMMSVLQNSFANSSSSLIQSKVTGNLVFVLLAPLSHVEFFA
                     AYVLASVARGLLVGLGVLAVTAWFVELEVVAPLWALAFALLGSMLLGVLGLLAGLWAE
                     KFDQIAAFQNFLILPLTMLSGVFYSIHSLPPAWQSVSHLNPFFYMIDGFRYGFFGVSD
                     FAPAASLAVVAVSLALLSALTLLLLDKGYKIRT"
     gene            2514..2753
                     /locus_tag="AC16O9_02825"
     CDS             2514..2753
                     /locus_tag="AC16O9_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR6515581.1"
                     /translation="MVTTESIESSIRAGLACERVEVLGDGQHFQALVVSAAFAGKSRV
                     QRHQLVYAAMGERMREEVHALSMKTLTPEEAAAGG"
     gene            2746..3999
                     /gene="murA"
                     /locus_tag="AC16O9_02830"
     CDS             2746..3999
                     /gene="murA"
                     /locus_tag="AC16O9_02830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6515582.1"
                     /translation="MDKLLVTGGRPLAGEARVSGAKNAALPILCAALLTEKPLALENV
                     PRLMDVRTMARLLAQMGVEVGAQSDGRLELRAKGIREPVAGYEMVKTMRASVLVLGPL
                     LARCGRARVSLPGGCAIGARPVDQHVKGLQAMGAEITVEHGYMNAEAKRLRGARVTMD
                     MVTVTGTENLMMAATLAEGSTVLENAAREPEVVDLADCLKAMGAKIEGAGTDVLRIEG
                     VSSLSGARHRVMPDRIETGTYLAAVAAAGGKVRLTGAAPDTVGATVEKLVEAGARIAL
                     GADSIEIEMAGRARAVSLATAPYPGFATDMQAQFMALDCIAAGTGTITETIFENRFMH
                     ALELQRLGADIAIQGNTAVVRGVQRLQGASVMATDLRASASLVIAGLVAQGETLIDRI
                     YHLDRGYEGLEQKLSALGARISRVR"
     gene            4056..4700
                     /gene="hisG"
                     /locus_tag="AC16O9_02835"
     CDS             4056..4700
                     /gene="hisG"
                     /locus_tag="AC16O9_02835"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR6515583.1"
                     /translation="MTGIGIALSKGRILEETGPLLARAGVAPKEDAERSRKLIIPTAR
                     RDVSLIIVRAADTPTYVAHGAADLGVAGRDVLEEHGGEGLYQPLDLGIARCRMMVAVR
                     KGFDYAGAVQQGARLRVATKYPRITRDFFADKGVHVDLIRLYGSMELAPIMGLADAIV
                     DLVSTGRTLKANKLAAVEEILPVSARLIVNQAALKTRRGELQPLIDAFAKATRP"
     gene            4697..5959
                     /gene="hisD"
                     /locus_tag="AC16O9_02840"
     CDS             4697..5959
                     /gene="hisD"
                     /locus_tag="AC16O9_02840"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6515584.1"
                     /translation="MKLRRLQTTDAGFERKVAALTRYDAAQDPAVQKAVRRILAEVRR
                     RGDAALRAYSKKLDGFLPKQFAITPDLADAIPPEQARALRAAHERIRAFHEKQLQQSW
                     EYTDAEGNRLGQRISPLARVGLYVPGGKAAYPSSVLMNAVPAKVAGVGELVMACPNPN
                     PLVLAAAALAGVDRVVRMGGAQAIAALAYGTKSIARVDKIVGPGNAYVAEAKRQVFGD
                     VGIEMVAGPSEILVIADGSAPADWVAMDLFSQAEHDESAQAILLSPDAGVLDAVEAAV
                     GRLLPGMARRQVIAASLKARGAFILTRDIEEACAVANRIAPEHLELLVEDADRWLPQL
                     TRAGAVFLGSYSSEAIGDYCAGPNHVLPTAGTARFSSPLGVYDFQKRTSVIGVSQAGA
                     RTLGRLAATLAEGEGLQAHARSAELRFK"
     gene            5956..7011
                     /gene="hisC"
                     /locus_tag="AC16O9_02845"
     CDS             5956..7011
                     /gene="hisC"
                     /locus_tag="AC16O9_02845"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6515585.1"
                     /translation="MTPDQLIRPEILALKAYHVADSDGMVKLDAMENPYPLPAPMRRE
                     LAEHLAQVDLNRYPDPTGRKLRALLARKMQVPAGMEILLGNGSDDLIQIVTFACARPG
                     AAMLFPAPTFVMYGMNATLSGMRALRHELRPDYTLDRDAFVAQVKAEQPALVFIAYPN
                     NPTGVLYPEEDVVAVLRAAEGLVVLDEAYHVFAQKSFMPRLAEFANLVVMRTVSKLGL
                     AGLRLGYLVGRPAWIQEFNKVRQAYNVNVLTQAAAEFVLERLEVLEAQAAAIRAERAV
                     LGKALAALPGVTVFPSEANFFLVRVPDADRSYEALRRQGVLVRNFNASGLRNCLRITV
                     GTPDENRILLTAMREAL"
     gene            7008..7598
                     /gene="hisB"
                     /locus_tag="AC16O9_02850"
     CDS             7008..7598
                     /gene="hisB"
                     /locus_tag="AC16O9_02850"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR6515586.1"
                     /translation="MNRTAEVTRSTKETQIRVRLDLDGKGAARLATGLPFLEHMLEQV
                     ARHGMLDLDVEAKGDLHIDAHHTVEDIGITLGQAFHKAIGDKAGIRRYGHAYVPLDEA
                     LSRAVIDFSGRPGLEYHVKFTRALIGEFDVDLVHEFFQGFANHAQVTLHIDNLRGDNA
                     HHQCETMFKAFARALRMAAEADARAAGVVPSTKGSL"
     gene            7599..8219
                     /gene="hisH"
                     /locus_tag="AC16O9_02855"
     CDS             7599..8219
                     /gene="hisH"
                     /locus_tag="AC16O9_02855"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR6515587.1"
                     /translation="MSIVVVDYGMGNIRSVSKALERVAPRARVAVTADAAAIRAAERV
                     VVPGQGALPDCMRQLAASGARDAVIEAARAKPFLGICIGMQMLFEHGEEGDTPGLGLL
                     AGTVPRFPAARMAGLKVPHMGWNEVRQARAHALWAGIPDAERFYFVHSYFPEPADGAL
                     TAAACAYGVAFTCAIARDNIFAVQFHPEKSQAAGLRLLSNFVQWRP"
     gene            8245..8991
                     /gene="hisA"
                     /locus_tag="AC16O9_02860"
     CDS             8245..8991
                     /gene="hisA"
                     /locus_tag="AC16O9_02860"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR6515588.1"
                     /translation="MLIIPAIDLKDGRCVRLKQGDMASATVFSEDPVAMAKHWAAQGA
                     RRLHIVDLNGAVAGRPKNEKVIRAMISAVGDAMPIQLGGGIRDLDTIESYIDAGVAFV
                     VIGTAAVKNPGFLSDASYAFPGRIIAGLDARDGKVAVEGWSKMTGHDVVDLAKKYQDY
                     GIEALVYTDIGRDGMMSGINVEATLKLAQAIKIPLIASGGLNTLKDVQEVCEKLVPEG
                     VIGAITGRALYEGKIEFKKAQAAADKAVGK"
     gene            9000..9761
                     /gene="hisF"
                     /locus_tag="AC16O9_02865"
     CDS             9000..9761
                     /gene="hisF"
                     /locus_tag="AC16O9_02865"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR6515589.1"
                     /translation="MSLAKRVIPCLDVTAGRVVKGVNFVELRDAGDPVEIAARYDAEG
                     ADELAFLDITASSDERDILLHVIEAVAAQVFIPLTVGGGVRKVEDVRRLLNAGADKVS
                     INTAAVQNPALVEQASGKVGNQCIVVAIDAKQKAGGGWEVYTHGGRKPTGLDAVDWAR
                     RMQAAGAGEILLTSMDRDGTREGFDLALTRAVCEAVGVPVIASGGVGTLEHLAAGVLE
                     GRADAVLAASVFHFGDFTVRRAKEHMRSRGIEVRL"
     gene            9758..10144
                     /gene="hisI"
                     /locus_tag="AC16O9_02870"
     CDS             9758..10144
                     /gene="hisI"
                     /locus_tag="AC16O9_02870"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR6515590.1"
                     /translation="MSWLDEVPWGKDGLITAVAQDAASGRILMVAWMNREALRQTAER
                     KEAVYWSRSRNRLWHKGEASGHVQKVLEVRLDCDADAVLLKVEQVAGIACHTGRESCF
                     FRKLEDGRWVSADPVLKDPTLIYGDQ"
     gene            10141..10473
                     /locus_tag="AC16O9_02875"
     CDS             10141..10473
                     /locus_tag="AC16O9_02875"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR6515591.1"
                     /translation="MKKGLAETLERIAAVIEKRRGGDPKTSYVARLLGPDEDAVLKKI
                     GEEATETVLAAKSGDRLHLVRETADLWFHCMILLAREGLGPGDVLAELHRREGISGID
                     EKAARKKR"
     gene            10470..10829
                     /locus_tag="AC16O9_02880"
     CDS             10470..10829
                     /locus_tag="AC16O9_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR6515592.1"
                     /translation="MSDANCLFCKIVRGEIPSKKVYEDEDVFAFHDIRPAAPVHFLII
                     PKAHVPTLYEADMAHHRALGKMLALAGELAKKEGAVDGFRTIVNTGRVGHQEVYHVHM
                     HVIGGPNSLGPMILKQK"
     gene            10838..11068
                     /gene="tatA"
                     /locus_tag="AC16O9_02885"
     CDS             10838..11068
                     /gene="tatA"
                     /locus_tag="AC16O9_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HIR6515593.1"
                     /translation="MGSFSIWHWLIVLVIVMLVFGTKKLRNMGADLGGAVRGFKDGMR
                     EGSDKSAEEGSTPQVTGKTVEGEVKSKTESKA"
     gene            11131..11496
                     /gene="tatB"
                     /locus_tag="AC16O9_02890"
     CDS             11131..11496
                     /gene="tatB"
                     /locus_tag="AC16O9_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR6515594.1"
                     /translation="MFELGFTELLVIALVALVVIGPERLPRVARTMGHLAGRLQRYVA
                     DVKADINREVELDELKKMRDSVQSAASDIESSVRAEMDKTESELNAAAAGKAEAAGKI
                     DAEVSPPPEAAAIEEKPKA"
     gene            11493..12245
                     /gene="tatC"
                     /locus_tag="AC16O9_02895"
     CDS             11493..12245
                     /gene="tatC"
                     /locus_tag="AC16O9_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR6515595.1"
                     /translation="MSSTQETFVSHLIELRQRVVHALIAFLVVFVAIFLYPGAAPIYD
                     FLASPLMKALPEGAKMIATGVVTPFLVPMKVTALVAFMIALPYLLYQAWAFVAPGLYE
                     HEKRLVLPVVIASTILFLLGVAFCYYFVFGKVFAFINDFAPKSITPAPDIEAYFSFVI
                     TMFLAFGITFEIPIVVMVLVRFGVVSLERLRDARPYVIVGAFVVAAIITPPDVLSQFM
                     LAVPMCLLYEAGLFLSRFVGKRTEEAKAAGET"
     gene            complement(12257..13444)
                     /locus_tag="AC16O9_02900"
     CDS             complement(12257..13444)
                     /locus_tag="AC16O9_02900"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR6515596.1"
                     /translation="MLHRLWSIFAQTATVCLAVLFVVTTLKPEWLPDRPAAAPPALPG
                     PPAVPGPLTTPGSVAPPAVMPAESFHDAVGRAMPSVVNVFTSKQVRARRNPLLNDPLF
                     RRFFGEFGDDVQRAESLGSGVIVSPAGYVLTNNHVVEAADEIEVALNDGRKLLAKVVG
                     SDPETDLAVLRVQGEQLPAISFGQSERLRVGDVVLAIGNPFGVGQTVTSGIVSALGRS
                     GLQINTFENFIQTDAAINPGNSGGALVDTRGDLVGINTAIYSRTGGSLGIGFAIPVST
                     AKMVLDQIVKSGTVTRGWIGVEVQEITPEVAESFRLGDARGTIVAGVLRGGPADRAGI
                     KPGDLLTAINGTPVSDPQAMLNLVAALAPGSSAKMRVQREAKTLELVVTVGKRPKPQL
                     REQ"
     gene            13580..14173
                     /gene="petA"
                     /locus_tag="AC16O9_02905"
     CDS             13580..14173
                     /gene="petA"
                     /locus_tag="AC16O9_02905"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR6515597.1"
                     /translation="MSDNPKVDKTRRNLVVATSVVGGAASVGAAIPFVTSMWPSERAK
                     AAGAPVQVDISELAPGELRVIEWRGKPVWVIRRTQDMLDALKVGLGQLTDPKSQSSMQ
                     PEYAANEYRSRKPEVMVMEGVCTHLGCSPQMKRADAKAEMGGDWPGGFYCPCHGSKFD
                     YAGRVYRGAPAPTNLPVPPYAFLSDSTLVIGEDKKGA"
     gene            14177..15460
                     /locus_tag="AC16O9_02910"
     CDS             14177..15460
                     /locus_tag="AC16O9_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6515598.1"
                     /translation="MASRLMALWDWFDSRMPSVAEEYRKHLAEYYAPKNFNFWYYFGS
                     LAMLVLVIQIVSGIFLTMHYKPDATLAFGSVEYIMRDVTGGWIIRYIHSTGASAFFVC
                     VYLHMFRALIYGSYKEPRELIWIFGVLIYLTLMGEAFFGYLLPWGQMSYWGAQVIVNL
                     FGTIPFIGPDLALWIRGDYVVSDATLNRFFALHVIALPLVLLGLLAAHILALHDVGSN
                     NPDGVEIKKNKDANGKPLDGVPFHPYYTVKDTLGAVVFLAIFCIVLFFMPELGGYFIE
                     YNNFIPADPLKTPEHIAPVWYFTPYYSILRAVPPLFDSQFPGVLAMGVSVMILFFLPW
                     LDRSPVKSIRYRGAYWKSAVAVFVIVFLVLGYLGVKPTTVWGQFGFDAFFLDTKDRAT
                     WAARVCTVLYFAFFILMPWYTARDKVKPVPDRVTM"
     gene            15472..16200
                     /locus_tag="AC16O9_02915"
     CDS             15472..16200
                     /locus_tag="AC16O9_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR6515599.1"
                     /translation="MKRFLAALLWAPATLLAAEGGYPLDRAPIEPRDLVSLQAGARTF
                     VNYCLNCHGAQYMRYNRLTEIGLSEPQIQDNLMFGAGKVGDTMKVAMSAKNGKDWFGV
                     PPPDLSVIARSRGADWLYTYLRAFYRDDTTPSGWNNALFPNVAMPHVLWTLQGERAYE
                     VVERTDKAGHVSLERKWKQRTPGTQSAVEYDRTVRDLVNFLVFVGEPAAQARKSIGIV
                     VLFVLGVLFVLAYAMKKAFWKDIR"
     gene            16271..16870
                     /locus_tag="AC16O9_02920"
     CDS             16271..16870
                     /locus_tag="AC16O9_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6515600.1"
                     /translation="MMQLYSGTTCPFSHRCRFVLYEKGMDFQVIDVDMFNKPEDLSVM
                     NPYNRLPVLVERDLILYESNIINEYIDERFPHPQLMPADPVMRARARLMLYNMEAELF
                     SQIDALDSGKDKQVEKARQQVTDRLIELSPVFTKTKHMLGDDFTMLDVAIAPLLWRLD
                     HYGIKLGKPAAPLMKYAERIFSRPAFIEALTPSEKVMRK"
     gene            16867..>17108
                     /locus_tag="AC16O9_02925"
     CDS             16867..>17108
                     /locus_tag="AC16O9_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein B"
                     /protein_id="HIR6515601.1"
                     /translation="MNEIATKPYLLRALYEWCVDNGYTPHLAVKVDSRTQVPSEYVKN
                     GEITLNVAPSAVHKLQIGNELIEFSARFAGVARQISV"
BASE COUNT         2739 a         5691 c         6000 g         2678 t
ORIGIN      
        1 gcggcggcgc tcgaccggta cgtgttcctg cgcgaggcgt acctgcagca gcgccgcagc
       61 cagatctatg atggccggcc gccgcgcgag cggccgccgg tggactgagg agaagcgcaa
      121 tgacacgaag gcttgcaggg gcggcgctgg cgctggcggc ggggttcgca ctcgccaatc
      181 cgctggcgcc cgacgccctg gtgaaggacg tgatgctgga catccagcgc atcatcgtcg
      241 cggacaagga cctgaaggaa ggcgatcgcg ccaagaccac ccgcctgatc gaggacaagg
      301 cgctgccgca cttcaaccac gtgggcatga cggcgcttgc ggtgggcgcg tcctggcgca
      361 aggccacgcc cgagcagaag cagcgcctgg ccgaggagtt ccgcacgctg ctggtgcgca
      421 cctacgccag ctcgctcagc gccttcggcg accagaagtt cgagttccgg ccgctgcgca
      481 tgaagccgga ggacaccgac gtcacggtgg aggtgcgcgt gctgcagccc ggctcgcagc
      541 ccgtggtggt cgagtacgac atggagaaga acgcccgcgg ctggcaggtc tatgacgtgc
      601 gcgtgggcgg catcagcctg gtggtcaatt accgcaccga gttcggcaac atcgtgcgcg
      661 acaagggcat cgacggcctg atcggcacgc tggcgacgaa gaacaagtcg ctcgccgccg
      721 ccgacacgaa gaaatgatcc gcgtcgacgg cgcgcgcctc tacgtcagcg gcccggcgac
      781 gctggcgacg gcggcgcagc tgctcgaggc ggcgcgcgcg ccgctcgcgg cgggcgtgcg
      841 cagcatcgac ctgggtgagg tcaccgagct cgactcgtcg ctgctcgcgg tgctgttcga
      901 gtggatgcgc gaggccaggg ccggcgggca ggcgctggtg ctcgagcggc tgccggcgga
      961 cctgaagagc ctggcgcagc tctacggcgt ggccgagctg ctgccgcaag acgccgcaag
     1021 ctgacccccg ccatcgaagt ccgcggcgtc gagaagcgct acggcgcgct gcaggcgctc
     1081 gccggcgtca cgctcgaggt gcgccagggc gaattcttcg gcctgctcgg cccgaacggc
     1141 gccggcaaga cgacgctgat caacatcatc gcggggctgg cgcgcgccag cgccgggtcg
     1201 gtggcggtgc tcggcgccga cgtggtggcg gactaccggc gcgcgcgcgc catgctcggc
     1261 gtggtgccgc aggagctggt gttcgacccg ttcttcaccg tgcgcgagac gctgcgcttc
     1321 cagtcgggct acttcggcat ccgcgccaac gacgcctgga tcgacgaggt gatgcaccac
     1381 ctcgacctcg acgccaaggc cgaagccaac atgcgctcgc tctccggggg catgaagcgg
     1441 cgcgtgctgg tggcgcaggc gctggtgcac aagccgccgg tgatcgtgct cgatgagccc
     1501 acggcgggcg tggacgtcga gctgcgccag ggcctgtggc agttcgtgcg gcggctgaat
     1561 cgcgacggcc acaccatcgt gctcaccacg cactacctgg aagaggccga ggagcactgc
     1621 cagcgcatcg cgatgctgaa ggccggccgc atcgtggcgc tcgacacgac gcagaacctg
     1681 ctcgcgagct ttgccgggcc gcacccggcg gacctcgagg acgtgttcct gcacctgatg
     1741 cagaagcact agcgcatgac gggcttcgtc acgctgctgt acaaagagct gctgcgcttc
     1801 tggaaagtcg cctaccagac cgtgggcggg ccggtgctct ccgcgctgct gtacgtcgtc
     1861 atcttctcgc aggcgctcgg cggccacgtg cgcgtgttcg agggcgtgcc ctacacggcg
     1921 ttcctggtgc cgggcctcgt gatgatgagc gtgctgcaga actcgttcgc caacagctcc
     1981 tcgagcctga tccagtccaa ggtcacgggc aacctggtgt ttgtgctgct cgcgccgctg
     2041 tcgcacgtgg agttcttcgc tgcctacgtg ctcgcctcgg tggcgcgcgg cctcctcgtc
     2101 gggctcggcg tgctcgcggt gaccgcgtgg ttcgtcgagc tcgaggtggt ggcgccgctg
     2161 tgggcgctcg cttttgcgct gctcggcagc atgctgctcg gcgtgctggg gctcctcgcg
     2221 ggcctgtggg cggagaagtt cgaccagatc gcggcgttcc agaacttcct catcctgccg
     2281 ctcaccatgc tctcgggtgt gttctactcg atccactcgc tgcccccggc ctggcagtcg
     2341 gtctcgcacc tgaacccctt cttctacatg atcgacggct ttcgctacgg cttcttcggc
     2401 gtgtccgact tcgccccggc ggccagcctc gcggtggtcg cggtaagctt ggcgcttctg
     2461 tcggcgctga ccctcttgct gctggacaaa ggctacaaga tcaggacctg accatggtga
     2521 caaccgaaag catcgaaagc agcattcgcg cgggcctcgc gtgcgagcgc gtcgaggtgc
     2581 tcggcgacgg ccagcacttc caggcgctgg tggtgtcggc ggcctttgcc ggcaagagcc
     2641 gcgtccagcg ccaccagctg gtgtacgcgg cgatgggcga gcgcatgcgc gaggaagtgc
     2701 acgcgctgtc catgaagacg ctcacgcccg aagaagcggc cgccggtgga taagctgctc
     2761 gtcaccggcg ggcgcccgct cgccggtgaa gcgcgcgtct ccggcgcgaa gaacgcggcg
     2821 ctgccgatcc tgtgcgcggc gctgctcacc gaaaagccgc tcgcgctgga gaacgtgccg
     2881 cgcctgatgg acgtgcgcac gatggcgagg ctcctggcgc agatgggcgt cgaggtcggc
     2941 gcgcagagcg acggccggct cgagctgcgg gcgaagggca tccgcgagcc ggtggccggc
     3001 tacgagatgg tgaagaccat gcgcgcgtcg gtgctcgtcc tcgggccgtt gctcgcgcgc
     3061 tgcgggcgcg cccgggtgtc gctgccgggc ggctgcgcca tcggcgcgcg cccggtggac
     3121 cagcacgtga agggcctgca ggcgatgggc gccgagatca cggtcgagca cggctacatg
     3181 aacgccgagg cgaagcgcct gcgcggcgcg cgcgtcacca tggacatggt gaccgtcacg
     3241 ggcaccgaga acctgatgat ggccgcgacg ctcgccgagg gcagcaccgt gctcgagaac
     3301 gcggcgcgcg agcccgaggt ggtggacctc gcggactgcc tgaaggcgat gggggcgaag
     3361 atcgagggcg cgggcaccga tgtcctgcgc atcgagggcg tgagctcgct ttcgggggcg
     3421 cggcaccgcg tgatgccgga ccgcatcgaa accggaacct atcttgccgc ggtggcggcc
     3481 gcgggcggca aggtcaggct gaccggcgcc gcgccggaca cggtcggtgc gacggtggag
     3541 aaactggtcg aggcgggcgc gcgcattgca ctcggtgccg acagcatcga gatcgagatg
     3601 gcggggcgcg ccagggcggt gagcctggcg accgcgccgt acccgggatt cgccaccgac
     3661 atgcaggcgc agttcatggc gctcgattgc atcgccgcgg gcaccggcac gatcaccgaa
     3721 accatcttcg agaaccgctt catgcacgcg ctcgagctgc agcggctggg cgccgacatc
     3781 gcgatccagg gcaataccgc cgtggtgcgg ggcgtgcagc gcctgcaggg ggcgagcgtg
     3841 atggcgaccg acctgcgcgc ctccgcgagc ctcgtaatcg cggggctggt ggcgcagggg
     3901 gagaccctca tcgaccgcat ctaccatctc gatcgcggct acgagggcct cgagcagaag
     3961 ctctcggcgc tcggcgccag gatctcgcgg gtaaggtaaa atcccgttcc gaatcaagcg
     4021 ggaggggccc ttcggggcct tttttcattt gcctcatgac cggcatcggc atcgcgctgt
     4081 ccaagggacg gatcctcgag gaaacggggc cgctgctcgc gcgcgccggc gtcgcgccta
     4141 aggaagacgc ggagcgctcc cgcaagctca tcatccccac cgcgcgccgt gatgtgagcc
     4201 tcatcatcgt gcgcgccgcc gacacgccga cctatgtcgc gcatggcgcc gccgacctcg
     4261 gcgtcgccgg cagggacgtg ctcgaggagc acggcggcga aggcctgtac cagccgctcg
     4321 acctgggtat cgcgcgctgc cgcatgatgg tggcggtgag aaagggcttc gactacgccg
     4381 gcgcggtgca gcagggcgcg cgcctgcgcg tggcgacgaa gtacccgcgg atcacgcgcg
     4441 acttcttcgc cgacaagggc gtgcacgtgg acctgatccg gctctacggc tcgatggagc
     4501 tggcgccgat catggggctc gccgacgcca tcgtcgacct ggtatccacc gggcggacgc
     4561 tgaaggccaa caagctcgcg gccgtggagg agatcctgcc ggtgtcggcg cgcctcatcg
     4621 tcaatcaggc ggcgctgaag acgcgtcgcg gcgagctgca gccgctgatc gacgctttcg
     4681 cgaaggccac gcggccatga aactgcggcg cctgcagacg acggatgccg gcttcgagcg
     4741 caaggtcgcg gcgctcacgc gctacgacgc ggcgcaggat cccgcggtgc agaaggccgt
     4801 gcgccggatc ctcgccgagg tgcgcaggcg cggcgacgcg gcgctgcgcg cctactcgaa
     4861 gaagctcgac ggcttcctgc cgaagcaatt cgccatcacg ccggacctcg ccgacgccat
     4921 cccgcccgag caggcgcgcg cgctgcgcgc ggcgcacgag cgcatccgcg cgttccacga
     4981 aaagcagctg cagcagtcct gggagtacac cgacgccgag ggcaatcgcc tcgggcagcg
     5041 catcagcccg ctcgcgcgcg tcgggctgta cgtgcccggc ggcaaggccg cgtacccgtc
     5101 ctcggtgctg atgaacgcgg tgccggcgaa ggtcgcgggc gtgggcgagc tggtgatggc
     5161 gtgcccgaac ccgaacccgc tggtgctggc ggcggcggcg ctcgcgggcg tggaccgcgt
     5221 cgtgcgcatg ggcggtgcgc aggcgatcgc cgcgctcgcc tacggcacga agtccattgc
     5281 gcgcgtggac aagatcgtcg gcccgggcaa cgcctacgtg gccgaagcga agcgccaggt
     5341 gttcggcgac gtgggcatcg agatggtggc ggggccgtcg gagatcctgg tcatcgccga
     5401 cggctcggcc cccgcggact gggtggcgat ggacctgttc tcgcaggccg agcacgacga
     5461 gtcggcgcag gcgatcctgc tgtcgccgga cgccggggtt ctcgacgccg tcgaagccgc
     5521 cgtcggcagg ctgctgccgg ggatggcgcg caggcaggtg atcgcggcct cgctcaaggc
     5581 gcgcggagcg ttcatcctca cgcgcgatat cgaagaggcc tgcgccgtcg caaaccgcat
     5641 cgcgcccgag cacctggaat tgctggtgga ggacgccgac cgctggctgc cccagctgac
     5701 gcgcgcgggc gccgtgttcc tcggcagcta cagctccgag gcgatcggcg actactgcgc
     5761 cgggccgaac cacgtgctgc cgaccgccgg cacggcgcgc ttttcctcgc cgctcggggt
     5821 gtacgacttc cagaagcgca cgagcgtgat cggcgtatcg caggccggcg cgaggacgct
     5881 cggacggctt gcggccacac tcgccgaagg cgaaggcctg caggcgcacg cgcgctcggc
     5941 cgagctgcgc ttcaaatgac gcccgaccag ctcatccggc ccgagattct cgcgctcaag
     6001 gcttaccacg tcgccgacag cgacggcatg gtgaagctcg acgcgatgga gaacccgtat
     6061 ccgctgcccg cgccgatgcg gcgcgagctc gccgagcatc tcgcgcaggt cgatctcaac
     6121 cgctacccgg atccgaccgg caggaagctg cgcgcgctgc tggcgaggaa gatgcaggtg
     6181 ccggcgggca tggagatcct gctcggcaac ggctcggacg acctgatcca gatcgtcacc
     6241 ttcgcctgcg cgcgccccgg cgcggcgatg ctctttccgg cgccgacctt cgtcatgtac
     6301 ggcatgaacg ccacgctctc gggcatgcgc gcgctgcgcc acgagctgcg cccggactac
     6361 acgctcgatc gcgacgcgtt cgtcgcgcag gtgaaggccg agcagccggc gctcgtcttc
     6421 atcgcctatc cgaacaatcc gaccggggtg ctctaccccg aggaagacgt ggtggcggtg
     6481 ctgcgcgccg ccgaggggct ggtggtgctc gacgaggcct accacgtctt cgcgcaaaag
     6541 agcttcatgc cgcggctggc cgagtttgcg aacctcgtgg tgatgcgcac ggtgtcgaag
     6601 ctggggcttg ccggcctccg cctcggctac ctcgtcggcc ggccggcgtg gatccaggaa
     6661 ttcaacaagg tgcgccaggc ctacaacgtc aacgtcctca cgcaggccgc ggcggagttc
     6721 gtgctcgagc gcctcgaggt gctggaggcg caggccgcgg cgatccgcgc cgagcgcgcg
     6781 gtgctcggca aggcgctggc ggcgctgccg ggcgtgacgg tatttccctc ggaggcgaac
     6841 ttctttctcg tccgggtgcc ggatgccgac cgcagctacg aagcgctgcg ccgccagggc
     6901 gtgctggtga ggaatttcaa cgcctccggc ctgaggaatt gcctgcgcat caccgtcggc
     6961 acgcccgacg aaaatcgtat actgctcacc gccatgcgag aagcgctgtg aaccgcaccg
     7021 ccgaggtaac gcgcagcacc aaggaaacgc agatccgggt aaggctcgac ctggacggca
     7081 agggcgcggc gcggctcgcc accggcctgc cgttcctcga gcacatgctc gagcaggtcg
     7141 cgcgccacgg catgctcgac ctcgacgtcg aggcgaaagg cgacctgcac atcgacgccc
     7201 accacacggt ggaggacatc ggcatcaccc ttgggcaggc gttccacaag gcgatcggcg
     7261 acaaggcggg catccgccgc tacggccacg cctacgtgcc gctggacgag gcgctgtcgc
     7321 gcgcggtgat cgacttctcc gggcggcccg gcctggaata ccacgtcaag ttcacgcgcg
     7381 cgctgatcgg cgagttcgac gtcgacctgg tgcacgagtt cttccagggc ttcgccaacc
     7441 acgcccaggt gacgctgcac atcgacaacc tgcgcggcga caacgcgcac caccagtgcg
     7501 agaccatgtt caaggcgttc gcgcgcgcgc tgcgcatggc ggcggaggcc gacgcgcgcg
     7561 ccgcgggcgt cgtgccttcc acgaaaggct cgctctagtt gagcattgtc gtagtcgact
     7621 acggcatggg caacatccgc tcggtgtcga aggccctcga gcgcgtggcg ccgcgtgcgc
     7681 gcgtcgccgt cacggccgac gccgccgcga tccgggcggc cgaacgcgtg gtggtgccgg
     7741 gacagggggc gctgcccgac tgcatgcgcc agctcgcggc gagcggcgcg cgcgacgcgg
     7801 tgatcgaagc ggctcgggcg aagcccttcc tcggcatctg tatcggcatg cagatgctgt
     7861 tcgagcacgg cgaggagggc gacacccccg gcctgggcct gctcgcgggc accgtgccgc
     7921 gctttcccgc cgcgcgcatg gcgggcctca aggtgccgca catgggctgg aacgaggtgc
     7981 gccaggcgcg cgcacacgcc ctgtgggcgg gcatcccgga cgccgagcgc ttctatttcg
     8041 tgcacagcta cttccccgag cccgccgacg gcgcgctgac ggcggcggcc tgcgcgtacg
     8101 gcgtcgcctt tacctgcgcc atcgcacgcg ataacatctt cgccgtgcag ttccacccgg
     8161 aaaaaagcca ggccgccggg ctccgtttgc tctccaactt cgtccaatgg cggccctagc
     8221 cctctaggtt tccggtagca ccgagtgctg atcatcccgg caattgacct gaaggacggc
     8281 cgctgcgtgc gcctgaagca gggcgacatg gcgagcgcga ccgtgttcag cgaggacccg
     8341 gtcgcgatgg cgaagcactg ggcggcgcag ggcgcgcggc gcctgcacat cgtcgacctg
     8401 aacggcgcgg tcgcgggacg gccgaagaac gagaaggtga tccgcgccat gatcagcgcg
     8461 gtgggcgacg cgatgccgat ccagctcggc ggcggcatcc gcgacctcga taccatcgag
     8521 agctacatcg acgccggggt cgccttcgtg gtcatcggca ccgcagcggt gaagaacccg
     8581 gggttcctct ccgacgcgag ctacgccttc ccggggcgga tcatcgccgg gctggacgcg
     8641 agggacggca aggtcgcggt cgaaggctgg tcgaagatga ccggccatga cgtcgtcgac
     8701 ctggcgaaga agtaccagga ctacggcatc gaggcgctcg tgtacaccga catcggccgc
     8761 gacggcatga tgagcggcat caacgtcgag gcgacgctga agctcgccca ggcgatcaag
     8821 atcccgctca tcgcctcggg cgggctgaac accctgaagg acgtgcagga ggtgtgcgag
     8881 aagctggtgc ccgaaggcgt gatcggcgcg atcaccgggc gtgcgctcta cgagggcaag
     8941 atcgaattca agaaggcgca ggccgcagcc gacaaggcgg tcggcaagta ggccgcgcca
     9001 tgagcctcgc caagcgcgtg atcccctgcc tcgacgtgac cgccgggcgg gtcgtgaagg
     9061 gcgtcaactt cgtcgagctg cgcgatgccg gcgacccggt cgagatcgcc gcgcgctacg
     9121 acgccgaggg cgccgacgag ctcgccttcc tcgatatcac cgcgagctcg gacgaacgcg
     9181 acatcctgct gcacgtcatc gaggcggtcg cggcgcaggt gttcatcccg ctcaccgtgg
     9241 gcggcggcgt caggaaggtc gaggacgtgc gccgcctgct caatgccggc gccgacaagg
     9301 tctccatcaa taccgccgcc gtgcagaacc cggcgctggt cgagcaggcc tcgggcaagg
     9361 tcggcaacca gtgcatcgtc gtcgccatcg atgcgaagca aaaggcgggc ggcggttggg
     9421 aggtctacac gcacggcggg cgcaagccca ctggcctcga cgcggtggac tgggcgcggc
     9481 gcatgcaggc cgcgggcgcg ggcgagatcc tcctgaccag catggaccgc gacggcacgc
     9541 gcgagggctt cgaccttgcg ctcacgcgcg cggtgtgcga ggcggtgggc gtgccggtga
     9601 tcgcctcggg cggcgtcggc acgctcgagc atctcgccgc gggtgtgctc gaaggccgcg
     9661 ccgacgcggt actggcggcg agcgtgtttc atttcggcga cttcaccgtg cgccgggcga
     9721 aggagcacat gcgctcgcgc ggcatcgagg tcaggctatg agctggctcg acgaggtgcc
     9781 gtggggcaag gacggcctga tcaccgcggt cgcgcaggat gcggcctcgg gccgtatcct
     9841 gatggtcgcc tggatgaacc gcgaggccct gcggcagacc gcggagcgca aggaggcggt
     9901 ctactggtcg cgctcgcgca acaggctctg gcacaagggc gaggcgtccg gccacgtgca
     9961 gaaggtgctg gaagtgcgcc tggattgcga tgccgacgcg gtgctgctca aggtggagca
    10021 ggtggcgggc atcgcctgcc acaccgggcg cgagagctgc tttttccgca aacttgaaga
    10081 cgggaggtgg gtgagcgcgg atccggtctt gaaggacccg acgctcatct acggggacca
    10141 atgaagaagg ggctggccga aacgctggag cgcatcgccg ccgtcatcga aaagcggcgc
    10201 gggggcgatc ccaaaacgtc gtacgtcgcg cggctgctcg ggcccgacga ggatgccgtg
    10261 ctgaagaaga tcggcgagga agccaccgag acggtgcttg ccgcgaagag cggcgaccgc
    10321 ctgcacctgg tacgcgagac cgccgacctg tggttccact gcatgatcct gctcgcacgc
    10381 gagggcctcg ggcccggcga cgtgctcgcc gagctgcacc ggcgcgaggg catctcgggc
    10441 atcgacgaaa aagcggccag gaagaagcga tgagcgacgc caactgcctg ttctgcaaga
    10501 tcgtgcgcgg cgagatcccg tcgaagaagg tctacgaaga cgaggacgta ttcgcgttcc
    10561 acgatatccg gccggcggcg ccggtgcact ttctcatcat ccccaaggcg cacgtgccga
    10621 ccctgtatga ggcggatatg gcgcaccacc gcgccctggg caagatgctg gccctcgccg
    10681 gcgagctggc gaagaaggag ggcgccgtgg acggctttcg caccatcgtc aacacgggcc
    10741 gtgtggggca tcaggaggtg tatcatgtgc acatgcacgt gatcggcggg ccgaactcgc
    10801 tcggccccat gatcctgaag cagaaatagg agggtttatg ggctcgttca gcatctggca
    10861 ctggctgatc gtcctcgtca tcgtgatgct ggtgttcggc acgaagaagc tgcgcaacat
    10921 gggcgcggat ctcggcggcg cggtgcgcgg gttcaaggac ggcatgcgtg agggcagcga
    10981 caaatcggcc gaggagggct cgactcccca ggtcaccggc aagactgtcg agggtgaagt
    11041 gaagagcaag accgagtcga aggcctgagc gccttcgcag cggcaacgac gcgggcgccc
    11101 tagggcgccc tttgtgtctc aaggccccgc atgttcgaac tgggatttac cgagctgttg
    11161 gtgatcgcgc tggtggcgct ggtggtcatc gggccggagc gcctgccgcg cgtggcgcgc
    11221 accatgggcc acctcgccgg ccgcctgcag cgctacgtcg ccgacgtcaa ggcggacatc
    11281 aaccgcgagg tcgagctcga cgagctgaag aagatgcgcg actcggtgca gagcgcggcg
    11341 agcgacatcg agtcctcggt gcgcgcggag atggacaaga cggagtccga gctgaacgcg
    11401 gcggccgcgg ggaaagccga ggccgcgggg aagattgacg ccgaagtttc gccgccgcca
    11461 gaggcggcgg cgatcgaaga aaagccaaag gcgtgagcag cacgcaggaa accttcgtct
    11521 ctcacctgat cgagctgcgc cagcgcgtgg tgcatgcctt gatcgccttc ctcgtcgtct
    11581 tcgtcgccat ctttctgtac ccgggcgccg caccgatcta cgattttctc gcctcgccgc
    11641 tgatgaaggc gctgcccgag ggcgcgaaga tgatcgccac cggcgtggtg acgccgttcc
    11701 tggtgccgat gaaggtgacg gcgctggtcg cgttcatgat cgcgctgccc tatctgctgt
    11761 accaggcctg ggcgttcgtc gcgccggggc tgtacgagca cgaaaagcgc ctggtgctgc
    11821 cggtggtgat cgcgagcacc atcctgttcc tgctcggcgt ggcgttctgc tactacttcg
    11881 tgttcggcaa ggtgttcgcg ttcatcaacg atttcgcgcc caagtcgatc acgccggcgc
    11941 cggacatcga ggcctacttc agcttcgtca tcaccatgtt cctcgccttc ggcatcactt
    12001 tcgagatccc gatcgtggtg atggtgctgg tgcgcttcgg cgtggtcagc ctggagcggc
    12061 tgcgcgacgc gcgcccctac gtcatcgtcg gcgcgttcgt ggtggcggcg atcatcacgc
    12121 cgcccgacgt gctgtcgcag ttcatgctgg cggtgccgat gtgcctgctg tacgaggcgg
    12181 ggctgttcct gtcgcgcttc gtgggcaagc gcactgaaga ggccaaggcg gcgggcgaga
    12241 cctagcgggc cgtcagctac tgctcgcgca gctgcggctt cgggcgcttg ccgaccgtca
    12301 ccacgagctc gagcgtcttc gcctcgcgct gcacgcgcat cttcgcgctc gagccgggcg
    12361 cgagcgccgc caccagattg agcatggcct gcggatcgga caccggagtg ccgttgatcg
    12421 cggtgagcag gtcgcccggc ttgatgccgg cgcggtcggc gggtccgccg cgcagcacgc
    12481 cggcgacgat ggtgccgcgc gcatcgccga gacggaacga ttcggccact tccggcgtga
    12541 tctcctgcac ttccacgccg atccagccgc gcgtcacggt gccgctcttc acgatctggt
    12601 cgagcaccat cttcgccgtg gatacgggga tggcgaagcc gatgccgagc gagccgccgg
    12661 tgcgcgaata gatcgcggtg ttgatgccca cgaggtcgcc gcgcgtgtcg accagcgcgc
    12721 cgcccgagtt gcccggattg atcgcggcat cggtctggat gaagttctcg aaggtgttga
    12781 tctgcaggcc gctgcgcccg agcgccgaga cgatgcccga ggtcacggtc tggccgacgc
    12841 cgaacgggtt gccgatcgcc agcaccacgt cgccgacgcg cagcctctcg gactggccga
    12901 agctgatggc ggggagctgc tcgccctgca cgcgcagcac ggcgaggtcg gtctccgggt
    12961 cgctgccgac caccttggcg agcagcttcc tgccgtcgtt gagcgccacc tcgatctcgt
    13021 cggcagcctc gacgacgtgg ttgttggtga ggacgtagcc ggccgggctg acgatcacgc
    13081 ccgagcccag gctctcggcg cgctgcacgt cgtcgccgaa ctcgccgaag aagcgccgga
    13141 acagcgggtc gttcagcagc ggattgcgcc gcgcgcgcac ttgcttgctg gtaaagacgt
    13201 tgaccaccga gggcatcgca cgcccaacgg catcgtggaa cgactctgcg ggcatcacgg
    13261 cgggcggcgc caccgacccc ggggtcgtga gcggccccgg gacggcgggc ggccccggga
    13321 gcgcgggcgg cgccgcggcg ggcctgtccg ggagccactc gggcttcagc gtcgtgacga
    13381 cgaacagcac cgccaggcac acggtcgccg tttgcgcaaa gatcgaccag aggcggtgca
    13441 gcataaggat cggctaatct agcacaaagc cctgcaaatt gaaccgctta gcacgcatat
    13501 cgtggacatc gtgacgctcg ctctatagaa tggcgcctcg tttgcactct cccacccttt
    13561 ccagcctcga atcaactcca tgagcgataa cccgaaagtc gacaagacgc gtcgcaatct
    13621 cgtagtcgcg acctcggtgg tcggcggcgc tgcctcggtc ggcgcggcga ttcctttcgt
    13681 caccagcatg tggccttcgg agcgcgcgaa ggccgcgggc gcgccggtgc aggtggacat
    13741 ctcggaattg gcgcccggcg agctgcgggt gatcgaatgg cgcggcaagc ccgtgtgggt
    13801 gattcgccgc acgcaggaca tgctggatgc gctcaaggtg ggcttggggc agctcaccga
    13861 cccgaagtcg cagtcgtcca tgcagcccga atacgccgcg aacgagtacc gctcgcgcaa
    13921 gcccgaagtc atggtcatgg agggcgtgtg cacgcacctg ggctgctcgc cccagatgaa
    13981 gcgcgcggac gcgaaggccg aaatgggcgg cgactggccg ggcggcttct attgcccctg
    14041 ccacggctcc aagttcgact acgccggccg cgtctaccgc ggggcgccgg cgccgaccaa
    14101 cctgccggtc ccgccatacg cgttcctgtc cgacagcacg ctcgtcatcg gcgaagacaa
    14161 gaaaggggcc taggcgatgg cttcccggct gatggcgttg tgggactggt tcgactcgag
    14221 gatgccctcg gtcgccgagg agtaccgcaa gcaccttgcg gagtactacg cgccgaagaa
    14281 cttcaacttc tggtactact tcggctcgct ggcgatgctg gtgctggtga tccagatcgt
    14341 gtcgggcatc ttccttacca tgcactacaa gcccgacgcc acgctcgcct tcggctcggt
    14401 cgagtacatc atgcgcgacg tcaccggcgg ctggatcatc cgctacatcc attcgaccgg
    14461 cgcgtccgcg ttctttgtct gcgtctacct gcacatgttc cgcgcgctga tctacggctc
    14521 gtacaaggag ccgcgcgagc tgatctggat cttcggcgtg ctgatctacc tgacgctgat
    14581 gggcgaggca ttcttcggct acctgctgcc ctggggccag atgtcctact ggggcgcgca
    14641 ggtgatcgtg aacctgttcg gcaccattcc gttcatcggc cccgacctgg cgctgtggat
    14701 ccgcggcgat tacgtggtct cggacgcgac gctcaaccgc ttcttcgcgc tgcacgtgat
    14761 cgcgctgccg ctggtgctgc tgggcctgct ggcggcgcac atcctggcgc tgcacgacgt
    14821 cggctcgaac aacccagacg gcgtcgagat caagaagaac aaggacgcga acggcaagcc
    14881 gctcgacggc gtgccgttcc atccgtacta cacggtgaag gacacgctgg gcgcggtggt
    14941 gttccttgcc atcttctgca tcgtgctgtt cttcatgccg gagctgggcg ggtacttcat
    15001 cgagtacaac aacttcatcc cggctgaccc gctgaaaacc ccggagcaca tcgcgccggt
    15061 gtggtacttc acgccctact actcgatcct cagggcggtg ccgccgctgt tcgactcgca
    15121 gttcccgggc gtgctggcga tgggcgtgtc ggtgatgatc ctgttcttcc tgccctggct
    15181 cgaccgctcc ccggtcaagt cgatccgcta ccgcggcgcg tactggaaga gcgccgtggc
    15241 ggtcttcgtc atcgtgttcc tggtgctggg ctacctgggc gtgaagccca ccacggtgtg
    15301 ggggcagttc ggcttcgacg ccttcttcct cgacaccaag gaccgcgcca cctgggcggc
    15361 gcgcgtgtgc accgtgctgt acttcgcctt cttcatcctg atgccgtggt acacggcgcg
    15421 cgacaaggtg aagccggttc ccgaccgggt gacgatgtga gggcccgtac cgtgaagcga
    15481 ttcctcgccg ccctgctctg ggcgccggcc acgctgctcg ccgccgaggg cggctacccg
    15541 ctcgatcgcg cgccgatcga gccgcgcgac ctggtcagcc tgcaggccgg cgcgcgcacc
    15601 ttcgtcaatt actgcctgaa ctgccacggc gcgcaataca tgcgctacaa ccgcctcacc
    15661 gagatcggcc tcagcgagcc gcagatccag gacaacctga tgttcggcgc cggcaaggtg
    15721 ggcgacacca tgaaggtggc gatgagcgcg aagaacggca aggactggtt cggcgtgccg
    15781 ccgccggacc tgtcggtgat tgcgcgctcg cgcggcgccg actggctgta tacgtacctg
    15841 cgcgccttct accgcgatga caccacgccg agcggatgga acaacgcgct gttcccgaac
    15901 gtggcgatgc cgcatgtgct gtggacgctg cagggcgagc gcgcctacga ggtcgtcgag
    15961 cgcaccgaca aggccgggca cgtgagcctg gagcgcaagt ggaagcagcg caccccggga
    16021 acgcagtctg ccgtcgaata cgatcgcacc gtgcgcgacc tcgtcaactt cctggtcttc
    16081 gtcggcgagc cggcggcgca ggcgcggaaa tcgatcggca tcgtcgtgct gttcgtgctc
    16141 ggcgtgctgt tcgtgctcgc ctacgcgatg aagaaggctt tctggaagga catccggtag
    16201 gcacgagcgc gcccgaaccc gcggccgggc gggcgcttgc gcccggccaa ttcctcagga
    16261 ctcctgcgac atgatgcaac tgtactccgg caccacgtgc cctttcagcc accgctgccg
    16321 cttcgtgctg tacgagaagg gcatggactt ccaggtgatc gacgtcgaca tgttcaacaa
    16381 gcccgaggac ctgtcggtga tgaatcccta caaccggctg ccggtgctgg tggagcgcga
    16441 cctcatcctg tacgagtcga acatcatcaa cgagtacatc gacgagcgct tcccgcaccc
    16501 gcagctgatg ccggccgatc cggtgatgcg cgcgcgcgcc cggctcatgc tctacaacat
    16561 ggaggccgag ctgttcagcc agatcgacgc gctcgactcg ggcaaggaca agcaggtgga
    16621 gaaggcgcgc cagcaggtca ccgaccgcct gatcgagctc tcgccggtgt tcaccaagac
    16681 caagcacatg ctgggcgacg atttcaccat gctggacgtg gccatcgcgc cgctcctgtg
    16741 gcgcctggat cactacggca tcaagctcgg caagcccgcg gcgccgctga tgaaatacgc
    16801 cgagcgcatc ttctcgcgcc cggcgttcat cgaggccctg acgccgtcgg aaaaagtaat
    16861 gcgcaagtga acgagatcgc caccaagccg tacctgctgc gcgcgctcta cgagtggtgc
    16921 gtggacaacg gctacacccc gcatctggcg gtgaaggtgg actcgaggac ccaggtgccc
    16981 tcggaatacg tgaagaacgg cgaaatcacg ctgaacgtgg cgccctcggc ggtgcacaag
    17041 ctgcagatcg gcaacgagct gatcgagttc tcggcgcgct ttgcgggcgt cgcgcgccag
    17101 atctcggt
//
LOCUS       DBNHKE010000050        34274 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_958955, whole genome shotgun sequence.
ACCESSION   DBNHKE010000050 DBNHKE010000000
VERSION     DBNHKE010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 34274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34274
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_958955"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC16O9_02930"
     CDS             <1..628
                     /locus_tag="AC16O9_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6515602.1"
                     /translation="AASGMQATVQTASPGATDYGSHIAAARARATEAWIAFGRAEDAA
                     EMVKAFKRARYAPPMFLAQGAAQEGFVRRVGQDAEFALGITAYEPGFATHGNAEFVAR
                     WRERWSGEPGLVAANAYSAALVLEAAVRAAGGLEQDRLRAALASLETETPIGRYRVGE
                     GGVQLGASPVVVQIQRGRAEIVWPRARATAQLRLPYPRWEQRQVHAPQ"
     gene            615..1514
                     /locus_tag="AC16O9_02935"
     CDS             615..1514
                     /locus_tag="AC16O9_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6515603.1"
                     /translation="MPLNRTYLFAPGNHPRRVEKALGLDADAVILDLEDAVALADKVA
                     TRQPVAAALKLPRRCRGYVRVNAPSTPFCYRDLVETIHPGVDGVFLPKVESAADLHAI
                     DWLLAALEREQGMAEGSLDLVPQLETAAGVARVDRILQAKNLRPYRGPWRVKRVAFGA
                     ADYCLELGLEPSLEEPELADARARIVLASRAAGAEGPLDSPWFHLRDTEGFRRALERS
                     RRGGFQGRLCVHPDQVALANAAFTPSADEAARAERIVAAFAAAEARGAAAVEVDGQMV
                     DYPIVYRARAVLETVREIAAKRG"
     gene            1548..2699
                     /locus_tag="AC16O9_02940"
     CDS             1548..2699
                     /locus_tag="AC16O9_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515604.1"
                     /translation="MTLAIQTLVAFAIYSTPVMAPVATREVGVQAAAIGYFIAAAYVG
                     SMLGSAAAGGWVARFGPIRVSQAGLALCLAGLALAASGWVPLMLLGAFVLGVGYGPTT
                     PASSVILVRAAPPAMFSLIFSLKQTGVPAGGVLAGALVPALVLALGWQGAALVIGASC
                     LALAVLIAPVRTRYDVGLNADAPVSMRAAFLPVRLVASHPRLREMAVTSFVYGGVQIT
                     LVTYLVTFLIDSFALSLVLAGAVMAVSQAASVLGRIAWGALADRAVTRRTMLGLLGLG
                     MGVSAMATLAAAPEWPRWVLFAFASIFGATAVGWNGVYLAEVARVAPPGQVSQATGGC
                     LFFTFLGVVLTPLIFNLVLALDGGYGVAYAVLGLPGLAMGARLLLRRPA"
     gene            2705..3325
                     /locus_tag="AC16O9_02945"
     CDS             2705..3325
                     /locus_tag="AC16O9_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6515605.1"
                     /translation="MRDAILVHGLWVPAAVMAPLAARLSADGLRCHLFSYAGRARPLA
                     AHAERLARYARDVGPAHFVGHSLGGLVALEALQAHAALKAGRVVLLGTPVRGNFAGRR
                     LGERGWGRWFLGATQPLWADGRAARWTRPEPLGVLAGTLPVGLGRLFGALPGPNDGVV
                     RVAETEVEGMRERVVLRVGHSAMLLSARVAAQVAAFLRDARFIAPR"
     gene            3312..4262
                     /locus_tag="AC16O9_02950"
     CDS             3312..4262
                     /locus_tag="AC16O9_02950"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR6515606.1"
                     /translation="MLLASAGALAGCETLAYYGQAAAGQLAISAQARPVAQVLADADV
                     APVLKERLRLASELRDYAVRVLKLPDSGAYRSYADLERPYALWNVVAAPEFALEPVQS
                     CFPIAGCVAYRGYFDRAGAERHGGARRAAGYDVVVYGVPAYSTLGWFDDPLLSTFIDY
                     PDAELARLLFHELAHQVAYVKDDSSFNESFAVVVEREGLRRWRGVPAAAHRREFEELV
                     LGLRSKLQALYASRLAPDEMRRRKREALEALRPWLKRMRGFESVEPSNAVLASFGTYT
                     QKVPAFERLLAEAGGDLERFYARVRELARLPRAERDARLR"
     gene            complement(4255..4737)
                     /locus_tag="AC16O9_02955"
     CDS             complement(4255..4737)
                     /locus_tag="AC16O9_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515607.1"
                     /translation="MEVNTTRCKECKGEMKLGTLAALAGEERGVRVRIEGMPAMQCAQ
                     GHKRFVAPDFAVRLMNALLADKQLDAPEAAKEKGLLRKRACCPKCGQELGEAGAGSVQ
                     ARRQLALPGLDAFGVDVELPKLRCASCGGESIPPQKTVVDGLMKATSSAFRSAQISPI
                     "
     gene            complement(4841..5596)
                     /locus_tag="AC16O9_02960"
     CDS             complement(4841..5596)
                     /locus_tag="AC16O9_02960"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HIR6515608.1"
                     /translation="MTRAAIVTGAASGIGLACAERLAADGMQVVLADVNEKAGTEQAK
                     RLGGTFFGADLTTRAGCRGLADAALKAYGGVHVLVNNAGFQHVAPIEEFSEDQWERMI
                     ALMLTAPFLLTRYCWPAMKRQKWGRVVNIASIHALVASPFKAGYVSAKHGLIGLTRTA
                     ALEGGEHGITVNAICPAYVKTPLVDGQIADQAKANNMSPADVIENIMLAPAAVKRLIE
                     PREVAEFVAYLCGEAAGTITGAALTMDLGWTAR"
     gene            complement(5593..6210)
                     /locus_tag="AC16O9_02965"
     CDS             complement(5593..6210)
                     /locus_tag="AC16O9_02965"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6515609.1"
                     /translation="MLRIWGRTNSVNVKKALWCAEELGLKYERIDAGMAFGVVNTPEY
                     RRMNPMGRVPTIDDDGFVLWESHSIVRYLCAKHSAGNLWPTDLMLRASAERWMDWAFT
                     FQAAMRDVFWGLIRTPPEQRDAKAIEAGCRQSAELAAMLDESLAARPYVAGASFTMGD
                     IPIGCEVQRWLRVPIERPSLPHLEAWFQRLRERKPFTKVVDQPLT"
     gene            complement(6213..7805)
                     /locus_tag="AC16O9_02970"
     CDS             complement(6213..7805)
                     /locus_tag="AC16O9_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6515610.1"
                     /translation="MTAFSWNNPYAWPRRPVLAKNVVATSQPLAAQAGLQMMAAGGNA
                     VDAILATAITLTLVEPVSNGIGSDAYAIVWDGKALHGLNASGRSPLGWTPEFFKDQKT
                     MPQRGWNSVTVPGCVSAWKMLAEKFGKLPFEKLFEAAIRYGREGFLVSPTIARQWAAQ
                     VPLLKDQPGFAEAFLPNGRAPQPGEVFRFKEHAATLEAIAKSKGEAFYNGPLAERLAS
                     HAKQNGGVLTVADMAAHTADWIAPLEMNYRGYTLHELPPNGQGIVALMALGMLEHFDV
                     KRHPVDGPDSVHLQIEAVKLAFADAWRYVADLEHMKDVKPPQMLDPEYLKERAKLIDM
                     KKAQDFGPGKPPKGGTVYLTAADASGMMVSFIQSNFTGFGSGVVVPGTGISLQNRGGT
                     FTLQPGHPNQVGPRKRPYQTIIPGFVTRDGKPVMSFGVMGGTMQPQGHTQVMVRIADY
                     GQHPQAACDGPRFRFVQGMDVSVEEGRFPAATLEELLNRGHKIVKIEDYNQFGCAQLV
                     WKTEGGYIAASDPRRDGQAVGF"
     gene            complement(7802..8869)
                     /locus_tag="AC16O9_02975"
     CDS             complement(7802..8869)
                     /locus_tag="AC16O9_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIR6515611.1"
                     /translation="MISYQIIEHGRPLQKVMHETPKPTGTEVLVRITRSGVCHSDVHI
                     WDGYFDWGGGKRFYVKERGCVPPFTLGHEPFGVVAALGPRARGVKVGQKRIVYPWIGC
                     GKCPVCKAGQDNYCMKAQFLGVMKPGGYSSHVLVPHPKYLVDASGIDEGFAATLGCSA
                     ITAYSGINKFPKLGPKDWVAVIGCGGLGLVALSILKAKGVRNVIACDVDEGKLAAAKK
                     QGAKLALDTRAPDAVQKLGALAMGNLAASIDFVGMPSTAMLGTGALRKGGRYVLCGLY
                     GGELQHPLPPIAQRAIGIVGSYVGNLQELKEVVALAKKKKLKPTPVTVRAFDEVNRTL
                     DELKAGKVLGRVVLDMEKETA"
     gene            complement(8866..10155)
                     /locus_tag="AC16O9_02980"
     CDS             complement(8866..10155)
                     /locus_tag="AC16O9_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR6515612.1"
                     /translation="MADRKFGFETLSLHAGQIPDPATGARAAPIYQTTSFVFDSAEHA
                     ASLFNLQTFGNVYSRISNPTVAVFEERIAALENGRAALACATGQSAEATAILTLCKAG
                     DHIVSASTLYGGTHTLLGVNLKKLGIDTTFVDPDDPANFRKALRPNTKLLYAETLGNP
                     LINIVDLAAVAAIAKDAGVPFLVDNTVPSPYLCRPFEHGADLVVHSATKYIGGHGTTM
                     GGAIVESGKFPWGNGKFPEFTTPSPGYHGVIFHETFGDFGFTMKARMEIMRTFGPTLS
                     PMNAWLLLQGVETLGVRMDRHCANALAVAKFLKEHKQVSWVNYPGLPGDKYHALAQKY
                     LPKGASGLLNFGVKGGAQAGERFIEAAQFMSHLANIGDAKTLIIHPASTTHRQMTDEE
                     QLKAGVTPDMIRMSVGIETLDDILWDIDQALTEAGKA"
     gene            complement(10172..11029)
                     /locus_tag="AC16O9_02985"
     CDS             complement(10172..11029)
                     /locus_tag="AC16O9_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6515613.1"
                     /translation="MRLQVVHETHYTYASPVLLSQQLLHLRPRALPWQKCLAHRLSVE
                     PDAGELAEREDYFGNPVAQLLIAAPHDQLLVRAASTLEVAPREQAALGLPRVPWEAAR
                     AQASAAAPDFVPESPHVETWRDLLEYAARCFPAGRAALDGAHELTHRIYRDFRFDKTA
                     TSVSTPLREVMKRRRGVCQDFAHLMTGCLRSLGLAARYVSGYLLTEPPPGRARLVGAD
                     ASHAWISAYCGEGAGWVDFDPTNDCIVDDEHVTLAWGRDFSDVTPMRGVILGGDAQEL
                     KVRVTVSPA"
     gene            complement(11040..13520)
                     /locus_tag="AC16O9_02990"
     CDS             complement(11040..13520)
                     /locus_tag="AC16O9_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6515614.1"
                     /translation="MPTELLAEYPRTTGRYDEMFAAPHAPRAHWQPLFAQLAAEPAER
                     MRERLHTVQRQVRENGVTYNVYADPQGADRPWELDLLPLVLPQDEWAAIEAAIVQRAT
                     LLNRILVDVYGEQRLLQEGLLPRALVHGNAGFLRPCRGVRVPGDVMLHLYAADLARSP
                     DGRWWVLHDRTQAPSGAGYALENRIVISRAFPELFHDLKVQHLASFFATLRDSLAHWA
                     PQDGSGAPPLTVLLTPGPHNETYFEHTYLARYLGLPLVEGSDLTVREGCVWLKTLSGL
                     KRVQAILRRQDDEYCDPLELRADSALGVPGLVDAVRRGNVLVANALGSNLLESSTLFG
                     FLPGICERLLGEPLAMPCVATWWCGEPAALEDVIGKMERLVIKAAFPQQRFEPMFGED
                     LDARGRKRAAAMLRARPNDYVAQELVQLSQAPVWDRAHPRRLLARAMGLRVYACASPN
                     GYVVMPGGLARVASADDARVISMQRGGSSKDAWVQAAGPVNTFSLLRRAVGPQDLVRA
                     GTNLSSRVVENLYWFGRYSERGDAMARLLRVAFGRLMDDAPADGTHGMAALVALLRSA
                     HVLPEGEARPADPELIVALRAAVVDASRPGLAHCLRDLSRVASHLRERLSLDNWRTLN
                     RMAREHGKRVRPPALADVLEALDRAIADFTTLSGFALDGMARDPGWRFLSVGRRIERL
                     QTLSATLRQALEGPADADLTWLLRLTDSIITYRARYTAQPQWLPVLDLLVRDEANPRS
                     LAFQINGLRDYARRLEEMFGDIGEEKLEGAAAALGALDPGADLRHGSAQFAAFLDECY
                     ASAYRFSEQLGHRFFSHVGASSRQTFAT"
     gene            complement(13528..14517)
                     /locus_tag="AC16O9_02995"
     CDS             complement(13528..14517)
                     /locus_tag="AC16O9_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6515615.1"
                     /translation="MQHVRFGPSGLHVSRLCLGTMTFGLQCDEKTSFAILDAAAGHGI
                     TFLDTANAYPLGGKLEDMGRTEAILGKWLKGKREQFVIATKCSGKMGDAPWQQGTSKK
                     HVMRAIDDSLKRLGTDYVDLYQVHHFDAVTPVDETVEAFDAVVKAGKARYVGVSNYHA
                     YRVARALGRAEALKLAKLVSVQPRYNLLFRQIERELLPLCQEENLAVIPYNPLAGGLL
                     TGKHKRGTPPAGTRFTLGAAAKTYTERYWREREFDTVDAFVRLAKEAGHEPATLAVAW
                     VLAHPAVTSPLIGASKPEQLKASVAAATLKLDPALKAKLDELSHEYRMGDAAR"
     gene            complement(14531..15274)
                     /locus_tag="AC16O9_03000"
     CDS             complement(14531..15274)
                     /locus_tag="AC16O9_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6515616.1"
                     /translation="MIARRKVLAVTGAAQGIGRAIALHFAQQGYAISFADTNVTAGRA
                     TLKALRSKGAKAEFFRADVSRESDVRNWLRATARTLGCPEVLVNNAGIRASAPFLTLS
                     PKAFDLVLATNLRGTFLCTQQAARLMVKHGKKGAIINIASTRAFMSEANTEAYSASKG
                     GIIALTHATAVSLGARGIRVNSISPGWIETRGAKHSRRDRLQHPAGRVGTPEDIARGC
                     FYIAEHAGFMTGQNLTLDGGMTVKMIYED"
     gene            complement(15271..18570)
                     /locus_tag="AC16O9_03005"
     CDS             complement(15271..18570)
                     /locus_tag="AC16O9_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6515617.1"
                     /translation="MSIHVALNHVTHYRYDRPVALSPHVVRLRPAPHARTPVHAYSLK
                     VAPAKHFLNWQQDPQANWLARLMFPEKTRELRVEVDLVAEMSVINPFDFFLEPIAEKI
                     PFVYDPAQLLELAPYLVRAPATPLFKAYLAEIPRAPRPSVDFLVALNSQLAKDIRYLI
                     RMEPGVQTPEETLQRASGSCRDSGWLLVQILRHLGLAARFVSGYLIQLTADVKSLDGP
                     SGPEADFTDLHAWCEVYLPGAGWIGLDPTSGLFAGEGHIPLACSPEPGSAAPVTGTTE
                     KCEAEFSHHMKVTRVWEAPRVTKPYTDAQWAAIEALGRRIDTDLVQGDVRLTMGGEPT
                     FVSLDDRDGAEWNTDAMGPTKRGLGARLLARLKARYAAQGLLHYGQGKWYPGEQLPRW
                     SLGCYWRKDGAPIWHDAALFADESVDYGATPEKAAAFLKALAARLGLGPGNVFPGYED
                     TWYYLWRERRLPSNVDPLDARLDDPLERERLRKVFSQGLDKVVGHVLPLQRDPDGRRW
                     QTGSWFLRAERCYLVPGDSPMGLRLPLDSQPWAAKADLPYVHAPDPMQPLAPLPRPLE
                     RSPHLPAPGAPPRAAAVRPPAMFESAAWITRMAMCAEPRHGVLYVFMPPAQSLEDYLE
                     LCAAIEATAAELTLPVVLEGYEPPKDPRLESLRITPDPGVLEVNVHPAKNWDELVEHT
                     THLYEQARETRLITEKFMLDGRHTGTGGGNHVVLGGSTAADSPFLRRPDLLASLLAYW
                     HNHPSLSYLFSGMFVGPTSQAPRIDEARNDSVYEIEVAFRELERQMKLSGAACPPWLV
                     DRLFRNLLIDASGNTHRSEFCIDKLFSPDGPTGRLGLLEMRAFEMPPHARMSLTQQLL
                     LRALVARFWQKPYLPGRLKRWGTELHDRFMLPWFVWQDFEDVIAEVSDFGYPLQAAWF
                     APHFEFRFPRYGEFAARGALVELRAALEPWHVMGEEGAPGGTVRYVDSTVERLQVLAS
                     GIVGDRMVLTCNGRRVPLTPTGNVGEFVGGVRYRAWQAPSALHPTIGVHSPLVFDLAD
                     TWMSRSMGGCQYHVSHPGGRAHATFPVNAYEAEGRRLARFFRIGHTPGRVEVASEARN
                     PNFPFTLDLRR"
     gene            complement(18572..19306)
                     /locus_tag="AC16O9_03010"
     CDS             complement(18572..19306)
                     /locus_tag="AC16O9_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6515618.1"
                     /translation="MKLNFLHEMRYSYESPVRYSTQYLRLTPRDTVRQKVLAWRLETP
                     GQPVGTHDGYGNVLHVLTLDKPVTGIVIRALGVVETARSVDEPSDFTGTPLSPLLFLR
                     ATPLTRADEKLAALAERYRRRAGTLSGLRELAAAAKQELPGAAAAELVHCFIACCRLL
                     GVPSRFVSGYLCAPGVVDAVAPHAWAEAWVVDRWRSFDIVQDSIVGEAHVKVAVGADY
                     NDACPIRGVRTGGGTETMSAVSQIVQ"
     gene            complement(19303..20241)
                     /locus_tag="AC16O9_03015"
     CDS             complement(19303..20241)
                     /locus_tag="AC16O9_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR6515619.1"
                     /translation="MLSRVADRIFWLSRQMERAENMARILGVTSNLVMFGTKDVQQQN
                     LLAPLSITGTEQAFFERHDKLTLAALIEFLALDEKNPSSIYCCLKGARENAHSVRWQI
                     TSEMWETLNGTWLEVRAMKPARITGAGATQFFDWVKDRSHLFRGVTYGTILRGEAFNF
                     SRLGTHLERADNTARILDVKYHVLLPSVADVGGALDFYQWTALLRSVSSFETYRQLYR
                     DQIYPIKVAQLLILERRMPRSLAACFDLIKDSMDRIKGQNDQAAKRLAYELHARLTHA
                     DIEEIFQSGLHEYLNACLADINELGSRIERAYLGAV"
     gene            complement(20241..21653)
                     /locus_tag="AC16O9_03020"
     CDS             complement(20241..21653)
                     /locus_tag="AC16O9_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6515620.1"
                     /translation="MPRRHYDEMRAEGGEIRAHYRALSDWLAETPAERVAEKRREADL
                     LFHRVGITFAVYGDEAGAERLIPFDTIPRVIPRQEWDMLERGLRQRVGALNRFLHDIY
                     HRQEILAAGVIPAEQVLTNDAYQVAMLGLDLPNEVYSHVAGIDLIRHDDGQYYVLEDN
                     LRTPSGVSYTLENRKMMMRLFPELFDRQHVYPVEHYPALLLETLRAAAPPRARDPVVV
                     VLTPGQYNSAYFEHAFLAQQMGVELVEGPDLFVKDGSVFMRTTTGSQRVDVIYRRVDD
                     AFLDPLAFRQDSYLGVPGLLSVYRAGNVGLANAIGTGVADDKSTYPYVPEMIRFYLGE
                     EPILKNVPTWQCKRPEDLAHVMDNLAELVVKEVQGSGGYGMLIGPAATKAEIEAFRKR
                     VHANPSNYIAQPTLSLSTCPTFVDKGIAPRHVDLRPFVLSGKEVRMVPGGLTRVALRE
                     GSLVVNSSQGGGTKDTWVVD"
     gene            complement(21702..22565)
                     /locus_tag="AC16O9_03025"
     CDS             complement(21702..22565)
                     /locus_tag="AC16O9_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6515621.1"
                     /translation="MRIFEGRFGRLILAEAPEFFGAGENTAPVLLFKHQGADCTLRVG
                     GESVPLTRDDVIFVEPLQVHAGEGTAGASGTRVIEFHPSPDWLHEAFPESFQPVGGRI
                     FARARETITPRMRQLADTLAIEALNDQFLSPERLEFMLQELLLSVVEHYLARRRAGAS
                     LWRGSRFADTRIRKALGLLRARPNKDVNIDDLASQVGLSRSRFYDLFHLSTGRSPRAY
                     LDMLCVETAIAKLSSGRARIADVSAELGFSAQSNFTRFFLSQVGIPPSEYRRAAARPE
                     GEEAPRPAEPV"
     gene            complement(22660..23130)
                     /locus_tag="AC16O9_03030"
     CDS             complement(22660..23130)
                     /locus_tag="AC16O9_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6515622.1"
                     /translation="MDDIHTLRRILSQNRVIAVVGLSADWFRPSYFAAKYMQEHGYAI
                     IPVNPRYAEILGQKCYRSLRDIPGKVDIVDCFRKSTDIMPICEDAIAIGAKVLWQQLG
                     VRNEAAAAKARAAGLEAVMDRCVKIEHGRLFGGLNWVGVNTRVISAKRPRWLAY"
     gene            23171..23512
                     /locus_tag="AC16O9_03035"
     CDS             23171..23512
                     /locus_tag="AC16O9_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0000423 - mitophagy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR6515623.1"
                     /translation="MYVEERMYLLHPGKAPGYFKHYEDVGMKVQLRHLPNLVGYYVTE
                     VGTQNLVTHMWAYDDLNQREKMRAAMQADPEWQAYLPRVRPLIITQETRIHKCAPFFV
                     ARLKKMLAAAK"
     gene            23509..24444
                     /locus_tag="AC16O9_03040"
     CDS             23509..24444
                     /locus_tag="AC16O9_03040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6515624.1"
                     /translation="MSLHPQVVQVLERAAKSPLPQLHEVPAHVARRIYRDTRAALSPR
                     PPEVAEAKLLMAPGPVALRAYRPADTGPADELPALVYFHGGGWVIGDLDTHDVLCRQL
                     ANGARCAVYSVDYRMGPEFPFPAAVEDCVTATRFVAERHKQVAVGGDSAGGNLATVVA
                     LQARDHGGPRIAYQLLIYPATDQRMQHPSIRKNGEGYLLTKKSMDYFQAQYLPRKEDR
                     VHWRASPLLAPRLAGLPPAHVITAGYDPLLDEGREYAARMAQEGVEVTYREYADMVHG
                     FILFGGVIDTANAAVAECCEKLRGCWQKEAHVVPQ"
     gene            24428..25054
                     /locus_tag="AC16O9_03045"
     CDS             24428..25054
                     /locus_tag="AC16O9_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515625.1"
                     /translation="MSSPNDLLWQVLFQFFIAVVLVFATVGLAAGVGLIVASARTLRV
                     FQVMNRWISTRGALRAMEIPRPTESFWHRHRRWIGWALIAGGTFAMLGLLLGVNTTAF
                     STAAAKGDMRMFVAIVAGTLKWFLIVGSAAGVVVGYLLCFSPEGLSILERHANRWISP
                     RRGMRGGDDSHAMLDKVVEAHPGPAGWILACTALGALIYAVTLFLARS"
     gene            25057..26739
                     /locus_tag="AC16O9_03050"
     CDS             25057..26739
                     /locus_tag="AC16O9_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6515626.1"
                     /translation="MQHDLKVRHGGRILADTLVGHGVKLAFGVPGESYLPVLDGFHDV
                     ADRLKFIVCRQEGGASYMAEAYGKLTGTPGVLFVTRGPGATNGAIGVHTGHQDSTPMV
                     VFVGQVGNDFVEREAFQEIDYRRMYGPLTKWVAQIDRVERIPEYVSHAFHTAMAGRPG
                     PVLLALPEDMLFSEAAVADVPHYKTVRPAPAPGDMRELQRLLQGAERPLVILGGGGWD
                     RATCDALRAWLEASRLAAGTSFRCQDLLDNRSASYAGDIGIGINPKLAQRIREADVLL
                     VIGARLGEMTTSGYTLLQAPVPSQTLIHVHGGAEELGRVYRAALPINAGMAQFVEALA
                     QVKLDGARWAARTKAAREEFVAWTEPRPMPGRLQYAEVVKWLDQHLPEDAIVCGGAGN
                     FSGWVHRYFRYKGFRTQLGSTAGSMGYGLPAAVAAKLAAPERTVIAVTGDGDFLMNGQ
                     ELATAAQYGAPVICLVVNNGMYGTIRMHQEREYPGRISGTELKNPDFAAYARAFGGHG
                     ELVETTDQFAPAFERARASGKPALIELRIDPEAITTSTTLSAVRQQALERRK"
     gene            complement(26743..27108)
                     /locus_tag="AC16O9_03055"
     CDS             complement(26743..27108)
                     /locus_tag="AC16O9_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR6515627.1"
                     /translation="MSKPCYDCGKCPAYCCSYPEIEVSKADIARLARHFGISAAAAEE
                     KFTKPQKEDNVRILRHREDEHFTSVCTFLDRKTRRCTVYEARPHTCRGYPYGARCGYY
                     EFLKFEREHQDDENFVATT"
     gene            27205..27924
                     /locus_tag="AC16O9_03060"
     CDS             27205..27924
                     /locus_tag="AC16O9_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515628.1"
                     /translation="MKFLQLAGAALALVLGTQAQAVGGLADVRVFDRSDGRQLPVYWH
                     RGQAYVVGKPGNEYRIIVRSRQGADLLAVMSVDGVNVITGQTAHPLQSGYVIGPRARL
                     AVDGWRKSLERTAAFYFTELNDSYAARTARPDNVGVIGVALYRRKAAPPQPFGGFAPA
                     PRAESRSSRESADAAAPLGTGHGRSETSYARHVEFERASAHPAETITIRYDSHRNLVA
                     MGVIPHAARQPVAFPGFVPDA"
     gene            27934..28470
                     /locus_tag="AC16O9_03065"
     CDS             27934..28470
                     /locus_tag="AC16O9_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6515629.1"
                     /translation="MQAPDEELMLAYREGDAGAFEALYTRHKGPLYRFVLRSVRERGT
                     AEELYQEIWMRVIEARSRYTVQAKFSTWLYTIAHNRLADHWRKRGLKLVALDGHDASA
                     PPADEPEARAAGRQGLQRFAAALAALPEAQRAAFLLHEESGLSVAEIAAATGANEETA
                     KSRVRYALAKLREAMSDD"
     gene            28463..29062
                     /locus_tag="AC16O9_03070"
     CDS             28463..29062
                     /locus_tag="AC16O9_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515630.1"
                     /translation="MTDPDRDARVSAHYRALGRDEPPAALDAEILAAARRPRARWAVP
                     VSVAAVVVLAVGVTLRLQLEERNGAEEVALSPRVMQAPAAAPEAAPPPRRDAAPAPAA
                     PMAREGRAQEPPVGARKREALAEASADRAQPAGRASASMAVGKITRTPETPEQWLERI
                     AQLREAGKGREADESLAAFRKRYPDYKLSDAMRARVAPR"
     gene            complement(29059..31998)
                     /locus_tag="AC16O9_03075"
     CDS             complement(29059..31998)
                     /locus_tag="AC16O9_03075"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR6515631.1"
                     /translation="MRAEPRLLLALFALSGFTGLVYESVWSHYLKLFLGAAAFAQSFV
                     LAVFMGGMALGAWLASRLSARWKNLLAAYGWIEALIGVAALSFHAAFTTLAALSLEHV
                     IPALSAPETIELYKYAVCALLIAPQTVLLGMTFPLMAGAVIRRFPRDGGGQPAGGRRL
                     ALLYFSNCIGAAAGALAAAFWLFGWLGLPGTLQLAGALNLALALAVWRLARGGEPRAA
                     APPAASAAAGARPGAPLVQLLLGAAFITGAASFVYEIAWIRMLSLVLGSSFQAFELML
                     SAFIAGLALGGLWMRRRIDRLDNPMRFSGVVQLAMGLAALGTIFLYQLSFDWMAWALR
                     VLQRSEEGYPLYLLFSHGVAFAVMLPATFLAGMTLPLFTHMLLRGGHGERAIGQVYAA
                     NTLGAIAGVLLAVHVLMPEAGLKVTLVVGAALDIVLGAWLLRWSGAVAQRAEAFAALI
                     LGLLAATATARAAALAPERLASGVFRYGQAEQRGEVLYYRDGKTATVAVVAAPSGART
                     ISTNGKPDAAIQMNPALPRTEDEYTMTLLGALPLLARPGAQRIANIGFGSGLTAETVL
                     SHDAVQALDIIEIEPAMAAGAYAFHPRVNQLFRDRRVRVHFEDAKSYFARHGKRYDAI
                     ISEPSNPWVNGVASLFTAEFYRDTARYLARDGLFVQWLHVYELDDRLLGSMFAALDTA
                     FADYDVYQVSAGDLVVVAVREGRVPLPGALPAAQPHLLDLLARIGITQREHVLARRLG
                     GKQALAPLFAQHAPPVNSDFRPLVQLEAPRARYLQQRAEALVELATAPLPVLEMLGGV
                     EATYLSAPAPRAGPGRYTLLVDALHLHHALLDRGTPTLDPQVLALRQSGALCGPLAGH
                     ALLDQLHGVASRTLTHLDPGRRRSLWVEPGWLGCPLEQAPAAARERFAVYRAIAERDA
                     HAMLARARAALENPSTPNPEWRRFLVLTAMLGAQAGGQGAEARRLWRLHGGMATPTER
                     YVADWRD"
     gene            complement(32019..32900)
                     /locus_tag="AC16O9_03080"
     CDS             complement(32019..32900)
                     /locus_tag="AC16O9_03080"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515632.1"
                     /translation="MATTIAFLGLGAMGSRMAINLQSAGYALRVYNRDRAKTRALVDK
                     GAQACDTPAAAVQGASFVVSMVADDVATRAVMLGADGAVAAATAGTIIIDSSTNTPAM
                     AREVAQAAAARRVQYLDAPVSGSLAQAQGKELVFMVGGERVAFERAQPVIAAMGRMAK
                     HMGAAGTGATIKLINNMLSAAATAATAEAVMIAEAAGLDRAATLEVLGEGAAGSRLMK
                     TRMPKMFSRDFAPQFQLQLMEKDLRYFQLLAQELDRPVPFAALVRSQFQAARRADLGK
                     LDSSALFLQAAGEKPKP"
     gene            32937..>34274
                     /locus_tag="AC16O9_03085"
     CDS             32937..>34274
                     /locus_tag="AC16O9_03085"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR6515633.1"
                     /translation="MPTPLWQPSEDAIEQAQITQFARHAVRKYRLDLNTYADFYQWTV
                     DNPELFWNEVWEWCGVKASRKGSTVLVDGDKMPGAKWFPEARLNFAENLLRRGDRGDA
                     FVFWDERGPQRRVSYSDLTSDVSRAVQALAQLGLRAGDRAAAFIPNLPDTAMLALAAL
                     SQGIVWSSCSPDFGVDGVIDRFGQIEPKVLFVADGYIYNGKEHRVLERVREIAERLPS
                     LRKVVVVPYLDARPDIEDVPKAVLLEEWLRRHTPGDIAWAQLPFDHPVYILFTSGTTG
                     KPKCIVHGAGGTLLAALKMYKLQFDVRPADHFFYYTTCNWVMWNLLFHGLAAEATVML
                     YDGSPFARDGKILFDYAQRERFSHFGTSAKFIDAIAKRGLKPREAYPLPGLRMIVSTG
                     SPLVAESYDYVYRDIKADVCLSSISGGTDIMAGFADANAALPVYRGELQCRSLG"
BASE COUNT         5021 a        11805 c        12281 g         5167 t
ORIGIN      
        1 ggcggcgagc ggcatgcagg cgacggtgca aacggcgtcc ccgggtgcga ccgactatgg
       61 ttcgcacatc gccgcggcgc gcgcgcgcgc cacggaagcc tggatcgcct ttgggcgggc
      121 cgaggacgcc gccgagatgg tgaaggcgtt caagcgcgcg cgctacgcgc cgccgatgtt
      181 cctggcgcag ggcgccgcgc aagagggctt cgtgcggcgc gtcgggcagg atgccgaatt
      241 cgcgctgggc atcacggcct acgagcccgg attcgccacg cacggcaatg ccgagttcgt
      301 ggcgcgctgg cgcgagcgct ggagcggcga gcccggcctc gtcgcggcca atgcctattc
      361 ggctgcgctg gtgctggagg cggccgtgcg cgccgcgggc gggctcgagc aggaccgcct
      421 gcgcgccgcg ctggcgtcgc tggagaccga aacgccgatc ggccgctacc gcgtaggcga
      481 gggcggcgtg cagctcggcg ccagcccggt ggtggtgcag atccagcgcg ggcgcgcaga
      541 aatcgtctgg ccacgcgcgc gcgctacggc acaattgcgg cttccgtacc cgcgctggga
      601 gcaaaggcag gtgcatgccc ctcaatagga cctacctgtt cgcgcccggc aatcacccgc
      661 ggcgcgtgga gaaggcgctc gggctggacg ccgatgcggt gatcctcgac ctggaggacg
      721 ccgtggcgct cgccgacaag gtcgccacgc gccagccggt cgccgcggcg ctgaaactgc
      781 cgcgccgctg ccgcggctac gtgcgggtga acgcgccctc gaccccgttc tgctaccgcg
      841 acctggtgga gaccatccac cccggggtgg atggcgtgtt ccttcccaag gtggagagtg
      901 cggccgatct gcacgccatc gactggctgc tcgcggcgct ggagcgcgag cagggcatgg
      961 ccgaaggctc gctcgacctg gtgccgcaac tggagaccgc cgcgggcgtg gcgcgagtgg
     1021 atcgcatcct gcaggcgaag aacctgcgcc cctaccgcgg gccgtggcgc gtgaagcgcg
     1081 tggcgttcgg cgcggcggac tattgcctgg agctcggcct cgagccctcg cttgaggagc
     1141 ccgagcttgc cgacgcgcgc gcgcgcatcg tgcttgcatc gcgcgcggcg ggcgccgaag
     1201 gcccgctcga cagcccctgg ttccacctgc gcgacaccga gggcttccgg cgcgcgctcg
     1261 agcgcagccg gcgcggcggc ttccaggggc gcctgtgcgt gcacccggac caggtggcgc
     1321 ttgccaacgc ggcgttcacg ccgagcgccg acgaggccgc gcgcgccgag cgcatcgtcg
     1381 ccgcgttcgc tgcagccgag gcgcgcggcg cggccgcggt cgaagtggac gggcagatgg
     1441 tcgactaccc gatcgtgtac cgcgcgcggg ccgttctcga gaccgtacgc gagatcgcgg
     1501 cgaagcgcgg ctagggtccc ctgggcggca tcttcctccc cgtcgcggtg acgctcgcga
     1561 tccagacgct ggtcgcgttt gcgatctaca gcacgccggt gatggcgccg gtggccacac
     1621 gcgaggtcgg cgtgcaagcc gccgccatcg gctatttcat cgccgcggcc tacgtcggca
     1681 gcatgctcgg ctcggcggcc gccggcggct gggtcgcgcg cttcgggccg atccgcgtca
     1741 gccaggcggg cctcgcgctt tgcctcgccg gcctggcgct cgccgcgagc ggctgggtgc
     1801 cgctcatgct gctgggggcg ttcgtgctgg gcgtgggcta cggacccacc acaccggcga
     1861 gctcggtgat cctggtgcgc gccgcgccgc ctgccatgtt ctcgctgatc ttctcgctga
     1921 aacagaccgg cgtccccgcg gggggcgtac tggccggggc cctcgtgccg gcgctggtgc
     1981 tcgcactggg ctggcagggg gcggcgctcg tcatcggcgc gagctgcctg gcgctcgcgg
     2041 tgctgatcgc tccggtgcgc acgcgctacg acgtggggct caatgccgat gcgcccgtgt
     2101 cgatgcgcgc ggcgttcctg ccggtgcggc tggtcgcgag ccacccgcgg ctgcgcgaaa
     2161 tggcggtgac ctccttcgtc tacggcggtg tgcagatcac gctggtcacc tatctggtga
     2221 ccttcctcat cgacagcttc gcgctgtcgc tggtgctcgc cggcgcggtg atggcggtat
     2281 cgcaggcggc gagcgtgctc gggcgcatcg cctggggcgc gctcgcagac cgcgccgtga
     2341 cccgccgcac gatgctcgga ctgctcggcc tgggcatggg cgtctcggcg atggcgacgc
     2401 tcgccgccgc gcccgagtgg ccgcgatggg tgctgtttgc gttcgcctcg atattcggcg
     2461 ccacggcggt aggctggaac ggcgtgtacc tcgcggaggt ggcgcgcgtc gcgcccccgg
     2521 ggcaggtcag ccaggccacc ggcgggtgcc tgttcttcac gttcctcggc gtagtgctga
     2581 cgccgctgat cttcaacctg gtgctggcgc tggacggcgg ctacggggta gcctacgcgg
     2641 tgctcggcct gccgggactc gcgatggggg cacggctact cttgcggcgt ccggcgtgac
     2701 gacgatgcgc gacgcgatac tggtgcacgg cctgtgggtg ccggcggcgg tgatggcgcc
     2761 gctcgccgcg cgcctgtcgg ccgacggctt gcgctgccat ctgttttcct acgccgggcg
     2821 cgcgcggccg ctcgcggccc acgccgagcg gctcgcgcgc tacgcgcgcg acgtcgggcc
     2881 ggcacatttc gtcggccaca gcctcggcgg gctggtggcg ctcgaggctc tgcaagcgca
     2941 cgcggcgctc aaggcggggc gcgtggtgct cctcggcacg ccggtgcgcg gcaacttcgc
     3001 cggcaggcgc ctgggcgagc gtggctgggg gcgctggttc ctgggcgcga cgcagccgct
     3061 gtgggccgat ggccgcgccg cgcgctggac tcggcctgag ccgctcggtg tgctcgccgg
     3121 aacgctgccg gtggggctgg ggcggctgtt cggcgcgctg ccgggcccga acgacggcgt
     3181 ggtgcgcgtg gcggaaaccg aggtcgaggg catgcgtgag cgcgtggtcc tgcgcgtcgg
     3241 ccacagcgcg atgctcctgt ccgcccgcgt ggccgcgcag gtggcggcct ttctgcgcga
     3301 tgcgcgcttt attgctcctc gctagcgccg gcgcgctcgc cggctgcgag acgctcgcct
     3361 actacgggca ggcggcggcc gggcagctcg ccatcagcgc ccaggcgcgg ccggtggcgc
     3421 aggtgcttgc cgatgcggac gtcgcgccgg tcctcaagga gcgcctgcgt cttgccagcg
     3481 agctgcgcga ctatgccgtg cgcgtgctca agcttccgga cagcggcgcc taccgcagct
     3541 acgccgacct cgagcggcct tacgcgctgt ggaacgtggt ggcggcgccc gagttcgcgc
     3601 tcgagccggt gcagtcctgc ttcccgatcg cgggctgcgt ggcgtaccgc ggctacttcg
     3661 accgcgctgg cgcagagcgc cacggcggcg cgcggcgcgc cgccggctac gacgtcgtcg
     3721 tctacggcgt gccggcgtac tccacgctcg gctggttcga cgatccgctg ctctcgacct
     3781 tcatcgacta cccggacgcg gaactggcgc ggctgctgtt ccacgagctc gcgcaccagg
     3841 tcgcgtacgt gaaggacgat tcgtcgttca acgagtcgtt cgccgtggtc gtcgagcgcg
     3901 aggggctgcg ccgctggcgc ggcgtcccgg ccgcggcgca ccggcgcgag ttcgaggaac
     3961 tggtgctcgg gctgcgctcg aagctgcaag ctctctacgc gtcccggctg gccccggacg
     4021 agatgcgccg ccgcaagcgc gaggcgctcg aggccctgcg gccgtggctc aagcgcatgc
     4081 gcggtttcga gtcggtcgag cccagcaacg cggtgctggc gtcgttcggc acctatacgc
     4141 agaaagtgcc cgcgttcgag cgcttgctcg ccgaggcggg cggcgacctc gagcgcttct
     4201 acgcgcgcgt gcgcgagctc gcgcgcctgc cgcgcgccga gcgcgacgcg cgcctcagat
     4261 aggcgagatc tgcgccgagc ggaaggcgct ggacgtggcc ttcatcaggc cgtcgaccac
     4321 ggtcttctgc ggcgggatgc tctcgccgcc gcaggacgcg cagcgcagct tgggcagctc
     4381 gacgtccacg ccgaaagcgt ccagccccgg cagcgccagt tggcgcctgg cctgcacgct
     4441 gcccgcgccg gcctcgccga gctcctggcc gcacttcggg cagcaggcgc gcttgcgcag
     4501 cagtcccttc tccttcgccg cttcgggcgc atcgagctgc ttgtcggcga gcagcgcgtt
     4561 catcagccgg acggcgaaat cgggcgcgac gaaacgcttg tgcccctgcg cgcactgcat
     4621 ggcgggcatg ccctcgatcc gcacgcgcac cccgcgctcc tcgccggcca gagcggccag
     4681 cgtgcccagc ttcatctcgc ctttgcactc cttgcaacgc gtcgtattga cttccatcct
     4741 cccctccccg gcgcgctcca gtgtggcgcg cccatataga ggggaccgcc gccacgggca
     4801 gatgttccgc ggtgctttgc aatgctgtgg ggcaaaaaaa ctagcgggcg gtccagccca
     4861 ggtccatggt gagcgccgcg ccggtgatgg tccccgccgc ctcgccgcac aggtaggcaa
     4921 cgaactccgc gacctcgcgc ggctcgatca ggcgcttcac cgccgctggc gcgagcatga
     4981 tgttctcgat cacgtccgcc ggggacatgt tgttcgcctt cgcctggtcg gcgatctggc
     5041 cgtcgacgag cggcgtcttc acgtaggccg ggcagatggc gttgacggtg atgccgtgct
     5101 cgccgccttc cagcgcggcg gtgcgcgtga gtccgatcag gccgtgcttg gccgagacgt
     5161 agcccgcctt gaaaggcgac gcgacgagcg cgtgaatcga cgcgatgttg acgacgcggc
     5221 cccatttctg cctcttcatc gccggccagc agtagcgcgt gagcaggaac ggcgccgtga
     5281 gcatcaacgc gatcatgcgc tcccactgat cctcggaaaa ctcctcgatc ggcgcgacgt
     5341 gctggaagcc ggcgttattg acgaggacgt gcacgccgcc gtacgccttc agcgcggcgt
     5401 cggcgaggcc gcggcagccc gcgcgcgtgg tgagatcggc gccgaagaac gtgccgccga
     5461 ggcgtttcgc ctgttccgtg ccggccttct cgttgacgtc ggcgagcacc acctgcatgc
     5521 cgtcggcggc gagccgctcg gcgcaggcga gaccgatgcc gctcgccgcg ccggtgacga
     5581 tcgcggccct cgtcatgtca ggggctggtc gacgaccttc gtgaacggct tgcgctcgcg
     5641 cagccgctgg aaccacgcct cgaggtgcgg cagcgacgga cgctcgatgg gcacgcgcaa
     5701 ccagcgctgc acctcgcagc cgatcgggat gtctcccatg gtgaagctgg cgccggcgac
     5761 gtacggccgc gcggcgagac tctcgtcgag catcgcggcg agctcggcgc tttgcctgca
     5821 gcccgcctcg atcgccttcg cgtcgcgctg ttccggcggc gtgcggatca ggccccagaa
     5881 cacgtcgcgc atcgccgcct ggaacgtgaa cgcccagtcc atccagcgct cggcactcgc
     5941 gcgcaacatg aggtcggtcg gccacaggtt gccggcgctg tgcttcgcgc agaggtacct
     6001 cacgatactg tgcgattccc acagcacgaa gccgtcgtcg tcgatggtcg gtacgcgccc
     6061 catcgggttc atcctgcggt actcgggcgt gttgacgacg ccgaaggcca tgccggcgtc
     6121 gatgcgctcg tatttcagcc cgagctcctc ggcgcaccac agcgccttct tcacgttgac
     6181 cgaattggtc cgtccccaga tcctgagcat ggctagaacc ccacggcctg gccgtcgcgg
     6241 cgcggatcgc tcgcggcgat gtagccgccc tcggtcttcc agacgagctg cgcgcagccg
     6301 aactggttgt agtcctcgat cttgacgatc ttgtggccgc gattcagcaa ttcctcgagc
     6361 gtcgcggcgg ggaaccggcc ctcctcgacg ctcacgtcca tgccctgcac gaagcgaaag
     6421 cgcggtccgt cgcaggccgc ctgcgggtgc tggccgtagt cggcgatgcg caccatgacc
     6481 tgcgtgtggc cctgcggctg catggtgccg cccatgacgc cgaagctcat caccggcttg
     6541 ccgtccctgg tgacgaagcc cgggatgatg gtctggtagg ggcgcttgcg gggtcccacc
     6601 tggttcgggt gtcccggctg cagcgtgaac gtgccaccgc ggttctgcag cgagatgccg
     6661 gtgcccggca cgaccacgcc cgagccgaag cccgtgaagt tcgactggat gaacgacacc
     6721 atcatgccgg aggcgtcggc cgcggtgagg tagacggtgc cgcccttggg cggcttgccg
     6781 ggcccgaagt cctgcgcctt cttcatgtcg atgagcttcg cgcgctcctt cagatattcg
     6841 ggatcgagca tctgcggcgg cttgacgtcc ttcatgtgct cgaggtcggc cacgtagcgc
     6901 caggcgtcgg cgaaagcgag cttcaccgcc tcgatctgca ggtgcacgct gtccgggccg
     6961 tcgaccgggt ggcgcttcac gtcgaagtgc tcgagcatgc cgagcgccat cagcgcgacg
     7021 atgccctggc cgttcggcgg cagctcgtgc agcgtgtagc cccggtagtt catctcgagc
     7081 ggcgcgatcc agtcggcggt gtgcgccgcc atgtcggcaa ctgtcagcac gccgccgttc
     7141 tgcttggcgt gcgacgcgag ccgctcggcc aggggcccgt tgtagaaggc ctcgcccttg
     7201 gacttcgcga tcgcttccag cgtcgcggcg tgctccttga agcggaacac ctcgcctggc
     7261 tgcggcgcgc ggccgttcgg caggaacgct tccgcgaagc cgggctgatc cttcagcagc
     7321 ggcacctgcg cggcccattg ccgtgcgatg gtcggcgaga ccaggaagcc ctcgcgcccg
     7381 tagcggatcg ccgcctcgaa cagcttttcg aacggcagct tgccgaactt ctccgcgagc
     7441 atcttccagg ccgagacgca gcccggcacg gtgaccgagt tccagccgcg ctgcggcatc
     7501 gtcttctggt ccttgaagaa ctcgggcgtc cagccgagcg gcgagcggcc cgaggcgttc
     7561 aggccgtgca acgcctttcc gtcccagacg atggcgtagg catccgaccc gatgccgttg
     7621 gagaccggct cgaccagcgt cagcgtgatg gccgtggcga ggatcgcatc gacggcgttg
     7681 ccgcccgcgg ccatcatctg caggccggcc tgcgcggcga gcggctgcga ggtggcgacg
     7741 acgttcttcg ccagcaccgg ccggcgcggc caggcgtaag ggttgttcca gctgaaagcc
     7801 gtcacgcggt ttccttttcc atgtcgagca cgacgcggcc cagcaccttg cccgccttca
     7861 gctcgtccag ggtccggttc acttcgtcga acgctcgcac ggtcaccggc gtcggcttca
     7921 gtttcttctt cttcgccagt gccaccactt ccttcagctc ctgcaggttg cccacgtagg
     7981 agccgacaat gccgatggcg cgctgcgcga tcggcggcag cggatgctgc agctcgccgc
     8041 cgtacaggcc gcagagcacg tagcgcccgc ccttgcgcag cgcccccgtt ccgagcatcg
     8101 ccgtagacgg catgccgacg aagtcgatcg acgcggcgag gttgcccatc gccagcgcgc
     8161 cgagcttctg cacggcatcg ggcgcgcgcg tatcgagcgc gagcttcgcc ccctgcttct
     8221 tcgccgccgc gagcttgcct tcgtccacgt cgcaggcgat gacgttcctg acccctttcg
     8281 ccttgaggat ggagagcgca acgagcccca ggcccccgca gccgatcacg gcgacccagt
     8341 ccttcggccc gagcttgggg aacttgttga tgccgctgta ggcggtgatc gccgagcagc
     8401 cgagcgtcgc ggcgaagcct tcgtcgatgc ccgaagcgtc caccaggtac ttcgggtgcg
     8461 gaacgagcac gtgcgaggag tacccgcccg gcttcatcac gccgaggaac tgcgccttca
     8521 tgcagtagtt gtcctggccg gccttgcaca ccgggcactt gccgcagccg atccacggat
     8581 agacgatgcg cttttgcccg accttcacgc ccctggcgcg cgggccgagc gcggcgacca
     8641 cgccgaaggg ctcgtggccc agggtgaagg gcggcacgca gccgcgttcc ttcacgtaga
     8701 agcgctttcc cccgccccag tcgaagtagc cgtcccagat gtgcacgtcg gaatggcata
     8761 cgccggagcg cgtgatcctc accagcacct cggtgccggt gggtttcggc gtctcgtgca
     8821 tcactttctg cagcggcctg ccgtgctcga tgatttggta gctgatcatg ctttccccgc
     8881 ttccgtcagt gcttggtcga tgtcccacag gatgtcgtcg agcgtctcga tgcctaccga
     8941 catgcggatc atgtcgggcg tgacgcccgc cttcaattgc tcctcatccg tcatctgccg
     9001 gtgcgtggtc gaggccgggt ggatgatgag ggtcttcgcg tcgccgatgt tggcgaggtg
     9061 gctcatgaac tgcgccgcct cgatgaagcg ctcgccggcc tgcgcgccgc ccttgacgcc
     9121 gaagttgagg agccccgagg cgcccttggg caggtacttc tgcgccagcg catggtactt
     9181 gtcgcccggc aggcccgggt agttcaccca ggacacctgc ttgtgctcct tcaggaactt
     9241 cgccaccgcg agcgcgttgg cgcaatgccg gtccatgcgc acgcccaagg tctccacgcc
     9301 ctgcagcagc agccaggcgt tcatcggcga gagcgtgggg ccgaaggtgc gcatgatctc
     9361 catgcgcgct ttcatggtga agccgaagtc gccgaaggtc tcgtggaaga tgacgccgtg
     9421 gtagccgggc gagggcgtgg tgaactcggg gaacttgccg ttgccccacg gaaacttccc
     9481 cgactcgacg atcgcgccgc ccatggtggt gccgtgcccg ccgatgtact tggtcgccga
     9541 gtgcacgacg agatcggcac cgtgctcgaa cggccggcac aggtagggcg agggcaccgt
     9601 gttgtcgacg aggaacggca cgcccgcgtc cttcgcgatc gccgccaccg ccgcgaggtc
     9661 gacgatgttg atgagcgggt tgccgagcgt ctcggcgtac agcagcttgg tattgggcct
     9721 gagcgccttc cggaaattcg cgggatcgtc cgggtcgacg aacgtcgtgt cgatgccgag
     9781 cttcttcagg ttcacgccca gcagcgtgtg ggtgccgcca taaagggtgg aggccgacac
     9841 gatgtggtcg cccgccttgc acaaggtcag gatcgcggtg gcctcggccg actggccggt
     9901 ggcgcaggcg agcgccgcgc ggccgttctc cagggccgcg atgcgctcct cgaacaccgc
     9961 caccgtcgga ttggagatgc gcgaatagac gttgccgaag gtctgcaggt taaacaggct
    10021 cgccgcgtgc tcggccgagt cgaacacgaa cgaggtggtc tggtagatgg gcgcggcgcg
    10081 cgcgccggtg gcggggtccg ggatctggcc ggcgtgcagc gacagcgtct cgaacccgaa
    10141 cttgcggtcg gccatggcgc gattctaagg gctatgccgg cgaaacggtg acgcgcacct
    10201 tgagctcctg cgcgtcgccg ccgaggatca cgccgcgcat cggcgtgacg tcgctgaagt
    10261 cgcggcccca ggcgagcgtc acgtgctcgt cgtcgacgat gcagtcgttg gtggggtcga
    10321 agtcgaccca gcccgcgccc tcgccgcagt acgccgagat ccaggcgtgc gaggcatcgg
    10381 cgccgaccag gcgcgcgcgc cccggcggcg gttcggtaag caggtatccg ctcacgtagc
    10441 gcgcggcgag cccgagcgag cgcaggcacc cggtcatcag gtgcgcgaag tcctggcaca
    10501 cgccgcggcg ccgcttcatc acctcgcgca gaggggtgga gacgctggtc gcggtcttgt
    10561 cgaagcggaa gtcgcggtag atgcggtgcg tgagctcgtg cgcgccgtcc agcgccgcgc
    10621 gcccggcggg gaagcagcgc gccgcatact cgagcagatc gcgccaggtc tcgacgtgcg
    10681 gcgactcggg cacgaaatcg ggggccgcgg cgctcgcctg cgcgcgcgcg gcctcccagg
    10741 gcacgcgcgg caggccgagc gccgcctgct cgcggggcgc cacctccagg gtcgatgcgg
    10801 cgcgtaccag cagctggtcg tggggcgcgg cgatcagcag ctgcgcgacc gggttgccga
    10861 agtagtcctc gcgctcggcg agctcgcccg cgtcgggctc gaccgaaagg cggtgcgcca
    10921 ggcacttctg ccagggtagc gcgcgcggcc gcaagtgcag cagctgctgc gacagcaaca
    10981 ccgggctggc gtacgtgtaa tgcgtttcgt ggacgacctg caggcgcacg gcgctcgcgc
    11041 taggttgcaa acgtctggcg cgaggacgcg ccgacgtggc tgaagaagcg gtggccgagc
    11101 tgctcgctga agcggtaggc cgaggcgtag cactcgtcga ggaacgcggc aaactgcgcg
    11161 ctgccgtggc gcaggtcggc gccggggtcg agggcgccaa gcgcggcggc ggcgccttcg
    11221 agcttctcct cgccgatatc gccgaacatc tcctcgaggc gccgcgcgta gtcgcgcagg
    11281 ccgttgatct ggaaggcgag ggagcgcggg ttggcctcgt cgcgcacgag aagatcaagc
    11341 accggcagcc attgcggctg cgcggtatag cgcgcccggt aggtgatgat ggaatcggtg
    11401 aggcgcagca gccaagtcag gtcggcgtcc gccgggccct cgagcgcctg gcgcagcgtg
    11461 gccgagagcg tctgcagtcg ctcgatgcgc cggccgacgg agaggaaccg ccatccgggg
    11521 tcgcgcgcca tgccgtcgag cgcgaatccc gagagcgtgg tgaagtcggc gatcgcccgg
    11581 tccagcgcct cgagtacgtc ggcgagcgcc ggcgggcgca cgcgcttgcc gtgctctcgc
    11641 gccatgcggt tcagcgtgcg ccagttgtcg agcgacagcc gctcgcgcaa gtgcgaggcg
    11701 acgcgcgaca gatcgcgcaa gcagtgcgcg aggcccggcc gcgacgcatc gacgacagcg
    11761 gcgcgcagcg ccacgatgag ctcggggtcg gccggccgcg cctccccctc gggcagcacg
    11821 tgcgcgctgc gcagcagcgc caccagggcc gccatgccgt gggtgccgtc ggcaggggca
    11881 tcgtccatga ggcgcccgaa cgcgacgcgc agcagccgcg ccatggcgtc gccgcgctcc
    11941 gagtagcggc cgaaccagta caggttctcc accacccggc tcgacaggtt ggtgccggcg
    12001 cgcaccaggt cctgcggccc gaccgcgcgg cgcagcaggc tgaacgtatt cacgggcccc
    12061 gccgcctgca cccaggcgtc cttgctcgac ccgccgcgct gcatggaaat cacgcgcgcg
    12121 tcgtccgcgc tcgccacccg ggcgagcccc cccggcatca cgacgtagcc gttggggctg
    12181 gcgcacgcgt acacgcgcag gcccatcgcg cgcgccagga gccgccgcgg atgggcgcgg
    12241 tcccacaccg gggcctgcga gagctgcacc agctcctgcg ccacgtagtc gttggggcgc
    12301 gcgcgcagca tggccgcggc gcgcttgcgg ccccgcgcat cgaggtcctc gccgaacatg
    12361 ggctcgaagc gctgctgcgg aaaagcggcc ttgatcacca ggcgctccat cttgccgatc
    12421 acgtcctcga gcgcggcggg ctcgccgcac caccaggtgg cgacgcaggg catggcgagc
    12481 ggctcgccga ggagccgctc gcagatgccc ggcaggaatc caaacagcgt gctcgattcg
    12541 agcaggttcg agccgagcgc gttggcgacg agcacgttgc cgcggcgcac cgcgtccacc
    12601 agccccggga cgccgagcgc ggagtccgcg cgcaactcca gcgggtcgca gtactcgtcg
    12661 tcctggcgcc tgaggatcgc ctgcacgcgc ttcagccccg agagcgtctt cagccatacg
    12721 cagccttcgc gcaccgtgag gtcgctgcct tccacgagcg gcaggccgag gtagcgcgcg
    12781 aggtaggtgt gctcgaagta ggtttcgttg tgcggtcccg gcgtcagcag caccgtcagg
    12841 ggcggcgcgc cgctgccgtc ctgcggcgcc caatgcgcga ggctgtcgcg cagcgtggcg
    12901 aagaagctcg ccaaatgctg aaccttgagg tcgtggaaca gctccggaaa ggcgcgcgag
    12961 atgacgatgc ggttctcgag cgcgtagccc gcgccggacg gggcctgcgt ccggtcatgc
    13021 agcacccacc agcgtccgtc gggcgagcgc gccaggtcgg cggcatacag gtgcagcatg
    13081 acgtcgcccg gcacgcgtac gccgcggcat gggcgcagga atcccgcgtt gccgtgcacc
    13141 agcgcgcgcg gcagcagacc ctcctgcagc aggcgctgct cgccgtagac gtcgaccagg
    13201 atgcggttga gcagcgtggc gcgctgcacg atcgccgcct cgatcgccgc ccactcgtcc
    13261 tgcggcagga cgagcggcag caggtcgagc tcccagggcc ggtcggcgcc ctgcgggtcg
    13321 gcgtagacgt tgtaggtgac gccgttctcg cgcacctggc gctgcacggt gtgcaagcgc
    13381 tcgcgcatgc gctctgcggg ctcggccgcg agctgcgcga acagcggctg ccagtgcgcc
    13441 cgcggcgcat gcggcgccgc gaacatctcg tcgtagcgcc ccgtggttcg cgggtactcc
    13501 gcgagaagct ccgtcggcat cggctcgcta gcgcgcggcg tcgcccatgc ggtactcgtg
    13561 cgagagctcg tcgagcttcg ccttcagcgc cggatcgagc ttcagtgtgg cggcggcgac
    13621 gctcgccttg agctgctcgg gcttgctcgc gccgatgagg ggcgaggtga ccgccgggtg
    13681 cgcaagcacc caggcgacgg ccagggtggc cggctcgtgc cccgcctctt tcgccagcct
    13741 gacgaaggcg tccaccgtat cgaactcgcg ctcgcgccag tagcgctcgg tgtaggtctt
    13801 ggcggcagcc ccgagcgtga agcgcgtgcc cgcgggcggc gtaccgcgct tgtgcttccc
    13861 ggtgagcagc ccccccgcga gcgggttgta ggggatcacg gccagattct cctcctggca
    13921 caacggcagc agctcgcgct cgatctggcg gaacagcagg ttgtagcgcg gctgcaccga
    13981 gacaagcttc gccagcttga gggcctcggc gcggcccagt gcgcgcgcca cgcggtaggc
    14041 gtggtagttg gacacgccga cgtatctcgc cttgcccgcc ttcactacgg cgtcgaacgc
    14101 ttccacggtc tcgtccaccg gggtgacggc gtcgaaatgg tgcacctggt agagatcgac
    14161 gtagtcggtg cccaggcgct tgagcgaatc gtcgatggcg cgcatgacgt gctttttcga
    14221 ggtgccctgc tgccaggggg cgtcgcccat cttgccgctg cacttcgtcg caatgacgaa
    14281 ctgctcgcgc ttgcccttca gccacttgcc gaggatcgcc tcggtgcgcc ccatgtcctc
    14341 gagcttgccg ccgagcgggt aggcgttggc ggtgtcgagg aaggtgatgc cgtggccggc
    14401 cgcggcgtcc agaatggcga aggaggtctt ctcgtcgcat tgcaggccga aggtcatggt
    14461 gccgaggcac aggcgcgaga catgcaggcc gctggggccg aagcggacgt gctgcattgc
    14521 ggtttcctcc ctagtcctcg tagatcatct tcaccgtcat gccgccgtcg agcgtcaggt
    14581 tttgtccggt catgaagccc gcgtgctcgg cgatgtagaa gcagccgcgg gcgatgtcct
    14641 cgggcgtgcc gacgcggccc gccgggtgct ggagtctatc gcggcgcgag tgcttcgcgc
    14701 cgcgcgtttc gatccagccg gggctgatcg aattcacgcg gatgccgcgc gcgccgaggc
    14761 tgaccgccgt ggcatgggtg agggcgatga tgccgccctt ggaggccgaa taggcttcgg
    14821 tgttggcttc ggacatgaag gcgcgggtcg aggcgatgtt gatgatcgcg cctttcttgc
    14881 cgtgcttcac catcaggcgt gcggcctgct gcgtgcagag gaatgtgccg cgcaggttgg
    14941 tggcgagcac gaggtcgaag gctttgggcg acagcgtcag aaagggcgcg ctcgcgcgaa
    15001 tgccggcgtt gttgaccagc acctcggggc agccgagcgt gcgcgccgtg gcgcggagcc
    15061 agttcctgac gtcggactcc cgggagacgt cggccctgaa gaactccgcc ttcgcgccct
    15121 tgctgcgcag cgctttcagc gtcgcgcggc cggcggtgac attggtgtcg gcgaacgaga
    15181 ttgcgtagcc ttgctgggcg aagtgcagcg cgatcgcacg gccgatgcct tgcgcggcgc
    15241 cggtgacggc gaggactttg cggcgcgcga tcaccgtcgc aggtccagcg tgaacggaaa
    15301 gttcggattg cgcgcctcgc tcgccacctc cacccgaccc ggcgtatgcc cgatccggaa
    15361 gaaccgcgcc aggcgccggc cttcggcttc gtaggcgttg accgggaacg tcgcatgcgc
    15421 ccgcccgccc ggatgcgaga cgtggtactg gcatccgccc atcgagcgcg acatccaggt
    15481 gtcggccagg tcgaacacca ggggggaatg caccccgatg gtgggatgca gtgccgaggg
    15541 cgcctgccag gcgcgatagc gcacgccgcc gacgaattcg ccgacgttgc cggtcggcgt
    15601 caagggcacg cgccgcccgt tgcaggtgag caccatgcga tcgccgacga tgccgctcgc
    15661 caggacctgg agccgctcga ccgtggaatc cacgtagcgc acggtgccgc cgggcgcgcc
    15721 ttcctcgccc atcacgtgcc agggctccag ggccgcgcgc agctcgacga gcgcgccgcg
    15781 cgcggcgaac tcgccgtagc gcggaaagcg gaactcgaaa tgcggcgcga accaggctgc
    15841 ttgcagcggg tagccgaaat cgcttacttc cgcaatcacg tcctcgaagt cctgccacac
    15901 gaaccacggc agcatgaagc gatcgtgcag ctcggtgccc cagcgcttca ggcgccccgg
    15961 caggtacggc ttctgccaga agcgtgcgac cagggcgcgc agcagcagtt gctgcgtgag
    16021 cgacatgcgc gcatgcggcg gcatctcgaa ggcgcgcatc tccagcagcc ccagccgccc
    16081 cgtcggcccg tcgggcgaaa acagcttgtc gatgcagaac tccgagcggt gggtgttgcc
    16141 cgaggcgtcg atgagcagat tgcggaacag gcgatccacc agccacggcg ggcacgcggc
    16201 gccgctcagc ttcatctgcc gctcgagctc gcggaacgcg acctcgatct cataaacgga
    16261 gtcgttgcgc gcctcgtcga tgcgcggcgc ctggctggtc gggccgacga acattcccga
    16321 gaacaggtac gacagcgacg gatggttgtg ccagtaggcg agcaggctcg cgagcaggtc
    16381 cggccggcgc aggaacggcg agtccgcggc cgtcgaaccg ccgaggacca catggttgcc
    16441 gccgccggtg cccgtgtggc gcccgtcgag catgaacttc tcggtgatga gccgggtctc
    16501 gcgcgcctgc tcgtacaggt gggtggtgtg ctcgacgagc tcgtcccagt tcttcgccgg
    16561 atgcacgttc acttcgagca cgccggggtc gggcgtgatg cgcagcgatt ccagccgcgg
    16621 atccttcggc ggctcgtagc cttcgagcac caccggcagc gtcagctcgg ccgccgttgc
    16681 ctcgatcgcg gcgcacagct ccaggtaatc ctcgagcgat tgggccggcg gcatgaacac
    16741 gtagagcacg ccgtggcgcg gctcggcaca catggccatg cgcgtgatcc acgcggcgga
    16801 ttcgaacatc gccggcggcc gcaccgccgc ggcgcgcggc ggcgcgcccg gcgcgggaag
    16861 gtgcggcgag cgctcgagcg ggcgcggcaa cggcgccagc ggctgcatcg ggtcgggcgc
    16921 gtgcacgtac ggcaggtcgg ccttcgccgc ccagggctgg gaatcgagcg gcagccgcag
    16981 gcccatcggc gaatccccgg gcacgaggta gcagcgctcg gcgcgcagga accacgaccc
    17041 ggtctgccag cgccggccgt ctgggtcgcg ctgcagcggc agcacgtgcc ccaccacctt
    17101 gtccaggccc tgcgagaaga ccttgcgcag ccgctcgcgc tccagcggat cgtcgagccg
    17161 cgcatcgagc gggtcgacgt tcgagggcaa gcgccgctcg cgccacaggt agtaccaggt
    17221 gtcctcgtag ccgggaaaca cgttcccggg cccgagtccg agcctggcgg cgagcgcctt
    17281 gaggaaggct gcggccttct cgggcgtcgc gccgtagtcc accgattcgt cggcgaagag
    17341 cgccgcgtca tgccagatcg gcgcgccgtc ctttcgccag taacacccga gcgaccagcg
    17401 cggcagctgc tcgcccggat accacttgcc ctggccgtaa tgcagcagcc cctgcgcggc
    17461 atagcgcgcc ttgagccggg cgaggagccg cgcgcccagg ccgcgcttcg tcggtcccat
    17521 cgcgtcggtg ttccattcgg cgccgtcgcg atcgtccagc gacacgaagg tcggctcgcc
    17581 gcccatggtg aggcgcacgt cgccctgcac gagatcggtg tcgatgcgcc ggccgagcgc
    17641 ctcgatcgcg gcccactgcg cgtcggtgta gggcttggtg acgcgcggcg cctcccacac
    17701 ccgcgtgacc ttcatgtggt gcgaaaactc ggcttcgcac ttctcggtgg tgccggtgac
    17761 gggcgcggcg cttcccggct cgggcgagca ggcgagcggg atgtggcctt cccccgcaaa
    17821 caggcccgag gtggggtcga ggccgatcca gccggcgccc ggcaggtaca cctcgcacca
    17881 ggcgtgcagg tcggtgaaat cggcctccgg tcccgagggc ccgtcgagcg acttcacgtc
    17941 ggcggtgagc tggatcaggt agcccgagac gaagcgcgcg gccaagccca ggtgacgcag
    18001 gatctgcacc aggagccagc cggagtcccg gcacgagccg ctcgcgcgct gcagcgtttc
    18061 ctccggcgtc tgcacgccgg gctccatgcg gatcaggtag cggatgtcct tggccagctg
    18121 cgagttcagc gccaccagga aatccaccga cggccgcggc gcgcgcggga tttcggccag
    18181 ataggccttg aacagcggcg tcgccggcgc cctgacgaga tagggcgcga gctcgagcag
    18241 ctgcgccggg tcgtagacga acgggatctt ttccgcgatc ggctcgagga agaaatcgaa
    18301 cgggttgatg accgacatct cggccaccag gtccacttcc acccgcagct cgcgcgtctt
    18361 ctccgggaac atgaggcgcg cgagccagtt cgcctgcggg tcctgctgcc agttgaggaa
    18421 gtgcttggcc ggcgcgactt tgagcgaata ggcgtgcacg ggggtgcgcg cgtgcggtgc
    18481 cgggcgcagg cgcacgacgt gcggcgacag cgccaccggc cggtcgtagc ggtagtgcgt
    18541 gacgtgattc agcgcgacgt ggatcgacat gtcactgcac gatctgcgag accgcgctca
    18601 tggtttccgt gccgccaccg gtgcgcacgc cgcggatcgg gcaggcgtcg ttgtagtcgg
    18661 cgccgacggc gaccttgacg tgcgcttcgc ccacgatcga gtcctggacg atgtcgaagc
    18721 tgcgccagcg gtcgaccacc cacgcttcgg cccaggcgtg cggcgccacc gcatcgacga
    18781 ccccgggcgc gcagaggtat cccgacacga agcgcgacgg cacgccgagc agccggcagc
    18841 aggcgatgaa acagtgcacc agctcggcgg cagcggcgcc gggcagctcc tgcttcgccg
    18901 ccgcggcaag ctcgcgcagc cccgacagcg tgccggcgcg gcggcggtag cgctcggcta
    18961 aggcggcgag cttctcgtcg gcgcgcgtca gcggcgttgc gcgcaggaac agcagcggcg
    19021 agagcggcgt gccggtgaag tccgagggct cgtcgaccga gcgcgcggtc tccaccacgc
    19081 ccagcgcgcg aatcacgatt cccgtcaccg gcttgtccag cgtcagcacg tgcagcacgt
    19141 tgccgtagcc gtcgtgcgtg ccgaccggct gccccggggt ctcgaggcgc caggcgagca
    19201 ccttctggcg caccgtgtcg cgcggcgtga ggcgcaggta ctgggtcgag tagcgcaccg
    19261 gggattcgta gctgtagcgc atttcgtgca ggaagttgag cttcataccg cccccaggta
    19321 ggcgcgctcg atgcggctgc ccagctcgtt gatgtcggcg aggcaggcgt tgaggtactc
    19381 atgcagcccg ctctggaaga tctcctcgat gtcggcatgc gtgaggcgcg cgtgcagctc
    19441 gtaggcgagg cgcttggcgg cctggtcgtt ctgtcccttg atgcggtcca tcgagtcctt
    19501 gatcaggtcg aagcacgcgg cgagcgagcg cggcatgcgc cgctcgagga tcagcagctg
    19561 cgccaccttg atcgggtaga tctggtcgcg gtagagctgg cggtaggtct cgaacgagga
    19621 caccgaccgc agcagcgccg tccactggta gaaatcgagt gcgcccccga cgtcggcgac
    19681 cgagggcagc agcacgtggt acttcacgtc gaggatgcgc gccgtgttgt cggcgcgctc
    19741 gaggtgcgtg ccgaggcgcg agaaattgaa cgcctcgccg cgcaggatgg tgccgtaggt
    19801 cacgccgcga aaaagatgcg agcggtcctt cacccagtcg aagaactgcg tggcgccggc
    19861 gccggtgatg cgcgcgggct tcatcgcgcg cacctccagc caggtgccgt tcagcgtctc
    19921 ccacatctcg gaggtgatct gccagcgcac cgagtgcgcg ttctcgcgcg cgcccttcag
    19981 gcagcagtag atcgacgagg gattcttctc gtcgagggcg aggaattcga tcagcgctgc
    20041 cagcgtcagc ttgtcgtggc gctcgaagaa cgcctgctcg gtgccggtga tggagagcgg
    20101 ggcgagcaga ttctgctgct gcacgtcctt ggtgccgaac atcaccaggt tggaggtgac
    20161 gccgaggatg cgcgccatgt tctcggcgcg ctccatctgg cggctcagcc agaaaatgcg
    20221 gtcggcgacg cgcgagagca tcagtccacg acccacgtgt ccttggtgcc cccgccctgc
    20281 gaggagttga cgaccaagga tccttcgcgc agcgcgacgc gcgtcaggcc gccgggcacc
    20341 atgcgcacct ccttgccgga gagcacgaac ggacgcaggt cgacgtggcg cggggcgatg
    20401 cccttgtcga cgaacgtcgg gcaggtcgac agcgacagcg ttggctgcgc gatgtaattc
    20461 gaaggattgg cgtgcacgcg cttgcggaac gcctcgatct cggccttggt cgcggcgggg
    20521 ccgatcagca tgccgtagcc gcccgagccc tgcacctcct tcaccaccag ctcggcgagg
    20581 ttgtccatga cgtgcgcgag atcctcgggt cgcttgcact gccaggtcgg cacgttcttc
    20641 aggatcggct cttcgccgag gtaaaaacgg atcatctcgg gcacgtaggg gtaggtggac
    20701 ttgtcgtcgg cgacgccggt gccgatggcg ttggcgaggc cgacgttgcc ggcccggtac
    20761 accgacagca ggcccggcac gccgaggtag gaatcctggc ggaacgcgag cgggtcgagg
    20821 aacgcgtcgt ccacgcgccg gtagatcacg tcgacgcgct gggagccggt cgtcgtgcgc
    20881 atgaagacgc tgccgtcctt cacgaacagg tccggtccct cgaccagctc gacgcccatc
    20941 tgctgcgcga ggaaagcgtg ctcgaagtag gcgctgttgt actgccccgg cgtgagcacc
    21001 accaccaccg gatcgcgggc gcgcggcggc gccgcggcgc gcagcgtctc caggagcagc
    21061 gccgggtagt gctcgaccgg gtagacgtgc tggcggtcga agagctccgg aaacagccgc
    21121 atcatcatct tgcggttctc gagcgtgtag gacacgcccg agggcgtgcg caggttgtcc
    21181 tcgagcacgt aatactggcc atcgtcgtgg cggatgaggt cgatcccggc gacgtgcgaa
    21241 tacacctcgt tcggcagatc caggccgagc atcgccacct ggtaggcgtc gttggtgagc
    21301 acctgctcgg cggggatcac gccggcggcg aggatctcct ggcgatggta gatgtcgtgc
    21361 aggaagcggt tgagcgcgcc gacgcgctgg cgcaggccgc gctcgagcat gtcccattcc
    21421 tgccgcggga tgacgcgcgg gatggtgtcg aacggaatca gccgctcggc gcccgcctcg
    21481 tcgccgtaga cggcaaaggt gatgccgacg cggtggaaca gcaggtcggc ctcgcggcgt
    21541 ttctccgcca cgcgctcggc gggcgtctcg gccagccagt ccgaaagcgc acggtagtgc
    21601 gcgcggatct cgccgccctc ggcgcgcatt tcatcgtaat ggcggcgcgg catgcaccaa
    21661 gggtaacgcg cgctccaccg gcgcaccagc gccggcgccg ctcagaccgg ctcggcgggc
    21721 ctgggcgctt cctcgccttc gggccgcgcg gcagcgcgcc ggtactcgga gggcgggatg
    21781 ccgacctggc tgaggaagaa gcgggtgaag ttgctttgcg ccgagaagcc gagctcggcc
    21841 gagacatcgg cgatgcgcgc gcggccgctc gagagcttgg caatcgcggt ctccacgcac
    21901 agcatgtcga ggtaggcgcg cggcgagcgc ccggtgctca ggtgaaacag gtcgtagaag
    21961 cgcgagcgcg acagaccgac ctggctcgcc aggtcgtcga tgttgacgtc cttgttgggg
    22021 cgcgcgcgca gcagcccgag cgccttgcgg atgcgcgtgt cggcgaagcg cgagccgcgc
    22081 cacagcgagg cgccggcacg ccggcgcgcg aggtagtgct cgaccacgga cagcagcagc
    22141 tcctgcagca tgaattccag ccgctcgggc gacagaaact ggtcgttgag ggcctcgatc
    22201 gccagcgtgt cggcgagctg gcgcatgcgc ggcgtgatgg tttcgcgcgc gcgcgcaaag
    22261 atgcgtccgc cgaccggctg gaagctctcc gggaacgcct cgtgcagcca gtcgggcgag
    22321 ggatggaact cgatgacgcg cgtgccgctt gcgcccgccg tgccttcgcc cgcgtgcacc
    22381 tgcagcggct cgacgaagat cacgtcgtcg cgcgtcagcg gcaccgactc gccgccgacc
    22441 cgcagggtgc agtcggcgcc ctggtgcttg aacagcagaa ccggcgcggt attctcgccg
    22501 gcaccgaaga attccggcgc ttcggcgagg atcaggcggc cgaagcggcc ttcgaaaatg
    22561 cgcaagggtc gggacacgga cgtaaggata actgtcctgc gccgcccgcg ctaggggcgg
    22621 atttcacgca tttcatcgaa aaaacagata tctggagctc tagtaggcca gccagcgcgg
    22681 gcgcttggcc gagatcaccc gcgtattgac gccgacccag ttcaggccgc cgaacaggcg
    22741 cccgtgctcg atcttcacgc agcggtccat gaccgcctcg aggccggcgg cgcgcgcctt
    22801 tgcggccgcg gcctcgttcc tcacgccgag ctgctgccag aggaccttgg cgccgatggc
    22861 gatggcgtcc tcgcagatcg gcatgatgtc ggtgctcttg cggaagcaat cgacgatgtc
    22921 gaccttgccg gggatgtcgc gcagcgagcg gtagcatttc tgcccgagga tctccgcgta
    22981 gcgcggattc acggggatga tggcgtaacc gtgttcctgc atgtacttgg cggcgaaata
    23041 gctcgggcgg aaccagtcgg ccgacaggcc gacgacggcg atgacccggt tctgcgaaag
    23101 aatgcggcgc agtgtatgga tgtcgtccat ggcccggata ctataatcga ccggatccag
    23161 ggaggggccc atgtacgtcg aggagcgcat gtacctgctg catccgggca aggcgccggg
    23221 gtacttcaag cactacgagg acgtgggcat gaaggtgcag ctccggcacc tgccgaacct
    23281 ggtcggctac tacgtgaccg aggtcggcac gcagaacctg gtcacgcaca tgtgggccta
    23341 cgacgacctc aaccagcgcg agaagatgcg cgccgcgatg caggccgatc ccgaatggca
    23401 ggcgtacctg ccgcgcgtgc ggccgctgat catcacgcag gagacgagga tccacaagtg
    23461 cgcgccgttc ttcgtggcgc ggctgaagaa gatgctggcg gcggccaagt gagcctgcac
    23521 ccccaggtcg tccaggtcct cgagcgcgca gcaaaatcgc cgctgccgca gctccacgag
    23581 gtccccgcgc acgtcgcgcg gcgcatctat cgcgacacgc gcgccgcgct ctcgcccagg
    23641 ccgcccgagg tcgcagaggc gaagctcctg atggcgccgg ggccggtggc gctgcgcgcc
    23701 tatcgccccg cggacaccgg cccggcggac gagcttccgg cgctggtcta cttccacggc
    23761 ggcggctggg tcatcgggga cctcgatacg cacgacgtgc tgtgccgcca gctcgccaac
    23821 ggcgcgcgct gcgcggtgta ttccgtggac taccggatgg ggcccgaatt cccgtttccc
    23881 gccgcggtgg aggactgcgt gacggcgacg cgattcgtcg ccgagcggca caagcaggtg
    23941 gccgttggcg gcgacagcgc gggcggcaac ctcgccaccg tggtggcgct gcaggcgcgc
    24001 gatcatggcg ggccgcgaat cgcgtaccag ctgctcatct atcctgcgac cgaccagcgc
    24061 atgcagcacc cctcgatccg caagaacggc gagggctacc tgctgaccaa gaaatccatg
    24121 gattattttc aggcgcagta cctgccgcga aaggaagacc gcgtccactg gcgcgcttcc
    24181 ccgctgctgg cgccgcgcct tgccggcctg ccgcccgcgc atgtgatcac cgcgggctac
    24241 gatccgctgc tggacgaggg ccgtgaatac gccgcgcgca tggcgcagga gggcgtggaa
    24301 gtcacgtacc gtgagtatgc cgacatggtg cacggcttca tcctgttcgg cggcgtgatc
    24361 gataccgcga atgccgcggt ggccgagtgc tgcgaaaagc tgcgcggctg ctggcagaag
    24421 gaggcccatg tcgtccccca atgatctgct gtggcaagtg ctgttccagt tcttcatcgc
    24481 cgtggtgctg gtgttcgcca cggtcggcct ggccgccggg gtcgggctga tcgtcgccag
    24541 cgcgcgcacg ctgcgcgtct tccaggtgat gaaccgatgg atctccaccc gcggcgctct
    24601 aagggcgatg gagattccgc gccccaccga gtccttttgg caccgccacc ggcgctggat
    24661 cggctgggcg ctgatcgcgg gcgggacctt cgcgatgctc ggattgctcc tcggcgtgaa
    24721 tacgacggcg ttcagcacgg cggccgccaa gggcgacatg cggatgttcg tcgcgatcgt
    24781 ggccgggacc ttgaagtggt tcctgatcgt cggcagcgcc gcaggcgtcg tggtgggata
    24841 tctgctgtgc ttttccccgg agggcctgtc gatcctggag cggcatgcca atcggtggat
    24901 ttccccccgg cgcggcatgc gcggcggcga tgattcgcac gcgatgctcg acaaggtggt
    24961 cgaagcgcat cccgggccgg cgggctggat cctcgcctgt acggcgctgg gcgcgctgat
    25021 ctacgcggtc acgctgttcc tggcgcgctc gtgaccatgc agcacgacct gaaggtacgc
    25081 cacggcgggc gcatcctcgc cgacacgctg gtcgggcacg gggtaaagct cgcgttcggc
    25141 gtcccggggg aaagctacct gccggtgctc gacggctttc atgacgtcgc cgaccggctg
    25201 aaattcatcg tctgccggca ggaaggcggc gcctcctaca tggcggaggc ctacggcaag
    25261 ctgaccggca cgccgggcgt gctgttcgtt acgcgcggcc ccggcgccac caatggcgcc
    25321 atcggggtgc acaccggcca ccaggattcc acgcccatgg tggtgttcgt cgggcaggtg
    25381 ggcaacgatt tcgtcgagcg cgaggcgttc caggaaatcg actatcggcg catgtacggg
    25441 ccgctcacca agtgggtggc gcagatcgac cgggtcgagc gcattcccga gtacgtgagc
    25501 cacgccttcc acacggcgat ggcgggccgt cccggaccgg tgctgctggc gctgcccgag
    25561 gacatgctgt tctcggaggc cgcggtcgcc gacgtgccgc actacaagac ggtgcgcccg
    25621 gcgcctgcgc cgggcgacat gcgcgagctg cagcggctgc tgcaaggcgc ggagcgaccg
    25681 ctcgtgatcc ttggcggcgg cggctgggac cgcgcgacct gcgacgccct gcgcgcatgg
    25741 ctggaagcct cgcggctcgc ggccgggacg tcgtttcgct gccaggacct gctcgacaac
    25801 cgcagcgcga gctacgccgg cgacatcggc atcggcatca atcccaagct cgcgcagcgc
    25861 atccgcgaag ccgacgtgct gctcgtcatc ggcgcgcgcc tgggcgagat gaccaccagc
    25921 ggctacacgc tgctgcaggc gccggtgccg agccagacgc tcattcacgt gcacggcggc
    25981 gccgaggagc tcggccgcgt gtaccgcgcg gcgctgccga tcaatgcggg catggcgcaa
    26041 ttcgtcgaag ccttggcgca agtaaagctg gacggcgcca ggtgggcggc gcgcaccaag
    26101 gcggcgcgcg aggagttcgt cgcctggacc gagccgcggc cgatgcccgg ccggctgcag
    26161 tacgccgaag tggtgaagtg gctcgaccag cacctgcccg aggatgcgat cgtctgcggc
    26221 ggcgcgggca acttctccgg ctgggtgcac cgctactttc gctacaaggg ctttcgcacg
    26281 cagctcggct cgaccgccgg gtcgatgggc tacgggctgc cggcggcggt cgcggccaag
    26341 ctcgcggcgc ccgagcgcac ggtgatcgcg gtgacgggcg acggggattt cctcatgaac
    26401 ggccaggagc tcgccaccgc cgcgcagtac ggcgcgccgg tcatctgcct ggtggtgaac
    26461 aacggcatgt acggcacgat ccgcatgcac caggagcgcg agtacccggg ccgcatctcg
    26521 ggcaccgagc tgaagaatcc cgacttcgcc gcctacgcgc gcgccttcgg cgggcacggc
    26581 gagctggtcg agacgaccga tcagttcgcg ccggcgttcg agcgcgcgcg cgcctcgggc
    26641 aagccggcgc tgatcgagct gcgcatcgac cccgaggcca tcaccaccag caccaccctc
    26701 agcgcggtgc gccagcaggc gctcgagcgc cgcaagtagg cttcaggtgg tggcgacgaa
    26761 gttctcgtcg tcctggtgct cgcgctcgaa cttgaggaac tcgtagtagc cgcagcgcgc
    26821 gccgtacggg tagccgcggc aggtgtgcgg ccgcgcctcg tacaccgtgc agcggcgcgt
    26881 cttgcggtcg aggaacgtgc acaccgacgt gaagtgctcg tcctcgcggt gccgcaggat
    26941 gcgtacgttg tcttccttct gcggcttggt gaacttttcc tccgcggccg ccgcgctgat
    27001 gccgaagtgc cgcgcgaggc gtgcgatgtc cgccttggag acctcgatct ccgggtagct
    27061 gcagcagtag gccgggcact tgccgcagtc gtagcagggc ttgctcatgg aaggcgcgca
    27121 tcttaaccct tttcgcgctc gaaatcgttt catgcaggga cggccggcca tcggggcggg
    27181 ccgcaccacg tgaaaggagc agccatgaaa ttcctgcagc tcgcgggcgc agcgctcgca
    27241 ctcgtcctcg gcactcaggc gcaggcggtc ggcggcctcg ccgacgtcag ggtgtttgat
    27301 cgcagcgacg gcaggcagct gcccgtgtac tggcaccggg ggcaggccta tgtcgtcggc
    27361 aagcccggca acgagtaccg catcatcgtg cgcagccgcc agggcgcgga cctgctggcg
    27421 gtgatgtcgg tggacggcgt gaacgtcatc acggggcaga ccgcgcaccc gctgcaaagc
    27481 ggctacgtca tcggcccgcg cgcgcgactg gcggtcgacg gctggcgcaa gagcctcgag
    27541 cgcaccgccg cgttctattt caccgagctg aacgattcct acgccgcgcg caccgctcgc
    27601 cctgacaacg tgggcgtgat cggcgtggcg ctctaccggc gcaaggctgc gccgccgcag
    27661 cctttcggcg gtttcgcgcc ggcaccgcgt gccgagtcgc gctcgtcgcg cgagtccgcc
    27721 gacgcggcgg cgccgcttgg caccggccac ggccgcagcg agacctcgta cgcgcgccac
    27781 gtcgagttcg agcgcgccag cgcgcacccc gcggagacca tcaccatccg ctacgacagc
    27841 caccgcaacc tcgtggcgat gggcgtgatc ccgcatgcag cacggcaacc ggtggcgttt
    27901 cccggcttcg tacccgacgc gtaaactcgc ggggtgcagg ccccggacga agagttgatg
    27961 ctcgcttacc gagaaggcga cgccggggcc ttcgaggcgc tctacacgcg gcacaagggg
    28021 ccgctgtacc gcttcgtgct gcgctcggtg cgcgagcgcg gcacggccga ggagctgtac
    28081 caggagatct ggatgcgggt gatcgaagca cgcagccgct ataccgtgca ggcgaagttc
    28141 agcacctggc tgtacacgat cgcccacaac cgcctggcgg accactggcg caagcgcggc
    28201 ctgaagctcg tcgcgctgga cggccacgac gcgagcgcgc cgcccgccga cgagccggaa
    28261 gcgcgcgccg cggggcgcca gggcttgcag cgcttcgccg cggcgctcgc cgcgctgccc
    28321 gaggcgcagc gcgcggcgtt cctgctgcac gaagaatcgg gactgagcgt cgccgagatc
    28381 gccgccgcca ccggcgcgaa cgaggagaca gcgaagagcc gcgtgcgcta tgcgctcgcc
    28441 aaactgcgtg aggccatgag cgatgactga tcccgaccgc gacgcgcgcg tctcggcgca
    28501 ctatcgcgcc ctcggccgcg acgagccgcc tgcggcgctc gacgcggaga ttctcgctgc
    28561 cgcacgccgc ccgcgcgcgc gctgggcggt gccggtgtcg gttgccgccg tggtcgtcct
    28621 cgctgtaggc gtgacgctgc gcctgcagct cgaggagcgc aacggcgcgg aggaagttgc
    28681 gctgtcgccg cgggtgatgc aggcgccggc cgcggcgccc gaggccgcgc ccccgccgcg
    28741 ccgcgatgcg gccccggccc cggcggcgcc gatggcgcgg gaaggccggg cgcaggaacc
    28801 gccggtcggc gcgcgcaagc gcgaggcgct cgccgaggcc agcgccgacc gcgcgcagcc
    28861 cgcgggccgg gcgagcgcca gcatggccgt cggcaagatc acgaggaccc cggagacgcc
    28921 ggagcagtgg ctggagcgca tcgcccagct gcgcgaggca ggcaaggggc gcgaggccga
    28981 tgagagcctc gctgcgttca ggaagcgcta tcccgactac aagctgtcgg acgcgatgcg
    29041 cgcgcgagtg gcgccgcgct aatcgcgcca gtcggccacg tagcgctccg tgggcgtggc
    29101 catgccgccg tgcagacgcc acaggcggcg cgcctctgcg ccctggccgc cggcctgcgc
    29161 cccgagcatg gcggtgagca ccaggaaacg tcgccactcg gggttcgggg tggaggggtt
    29221 ttcgagtgcc gcgcgggcgc gcgccagcat ggcgtgcgca tcgcgctccg caatcgcgcg
    29281 gtagacagcg aagcgttcgc gcgccgcggc gggcgcctgc tcgagcgggc agccgagcca
    29341 gcccggttcg acccacagtg accggcgccg ccccgggtcg aggtgcgtca gcgtgcgcga
    29401 tgccacgccg tgcagttgat cgagcagggc gtgccccgcg agcgggccgc atagcgcgcc
    29461 ggactggcgc agtgcgagca cctgcgggtc gagggtcggc gtgccgcgat ccagaagcgc
    29521 gtggtgcaag tgcagcgcat cgaccagcag cgtgtagcgt cccggcccgg cgcgcggcgc
    29581 cggcgccgaa aggtacgtcg cctctacgcc gccgagcatt tccagcacgg gcagcggcgc
    29641 cgtcgccagc tcgaccagtg cctcggcgcg ctgctgcagg tagcgcgcgc gcggcgcctc
    29701 cagctgcacc agcgggcgga agtcggagtt aaccggcggc gcgtgctgcg caaagagcgg
    29761 cgccagcgcc tgcttgccgc ccaggcgccg cgcgagcacg tgctcgcgct gcgtaatgcc
    29821 gatccgcgcc agcaggtcga gtaagtgcgg ctgcgcggcc ggcagcgcgc ccggcagcgg
    29881 cacgcgaccc tcgcgcaccg caaccacgac gaggtcgccg gcgctcacct ggtagacgtc
    29941 gtaatccgcg aaagccgtgt ccaaggccgc gaacatcgaa cccagcaacc ggtcgtcgag
    30001 ctcgtacacg tgcagccact gcacgaataa gccgtcccgg gcgaggtagc gcgcggtatc
    30061 gcggtagaac tccgccgtga acaggctggc gacgccgttg acccagggat tggagggctc
    30121 ggagatgatc gcgtcgtagc gcttgccgtg ccgggcgaaa tagctcttcg cgtcctcgaa
    30181 atgcacgcgc acgcgccggt cgcggaacag ctggttgacg cgggggtgga acgcgtaggc
    30241 gccggccgcc atcgccggct cgatctcgat gatgtcgagc gcctggaccg cgtcatgcga
    30301 gagcacggtt tccgcggtga gccccgagcc gaagccgatg ttggcgatgc gctgcgcgcc
    30361 gggccgggca aggagcggca gggcgccaag gagcgtcatg gtgtactcgt cctcggtgcg
    30421 cggcagcgcc gggttcatct ggatcgccgc atcgggcttg ccgttggtgc tgatggtccg
    30481 cgcgcccgaa ggcgccgcga ccaccgcgac ggtagccgtc ttgccgtcgc ggtagtagag
    30541 cacctcgccg cgctgctcgg cctggccgta gcggaacacg cccgaggcga ggcgctccgg
    30601 cgccagcgcg gcggcgcgcg cggtggcggt cgcggcgagc agcccgagaa tcagcgcggc
    30661 aaaggcctcg gcgcgctgcg caacggcgcc cgaccagcgc agcagccagg caccgagcac
    30721 gatatcgagc gcggcgccga cgacgagcgt caccttgagg ccggcttcgg gcatcagcac
    30781 gtgcacggcg aggagcaccc cggcgatcgc gcccagcgtg ttggcggcat acacctggcc
    30841 gatcgcgcgc tcgccgtggc cgccgcgcag cagcatgtgc gtgaacaagg gcagcgtcat
    30901 cccggcgagg aacgtcgccg gcagcatgac ggcgaacgcc acgccatggc tgaacaacag
    30961 gtacagcggg tagccctcct cggagcgctg cagcacgcgc agcgcccagg ccatccagtc
    31021 gaagctcagc tggtacagaa agatcgtgcc gagcgccgcc agccccatcg ccagctgcac
    31081 cacgccggag aagcgcatcg ggttgtccag ccggtcgatg cgccggcgca tccacagccc
    31141 gcccagcgca agcccggcga tgaacgccga gagcatcagc tcgaacgcct ggaacgacga
    31201 acccagcacc agcgacagca tgcggatcca ggcgatctcg tacacgaacg acgccgcgcc
    31261 ggtgatgaac gcggcgccga gcagcagctg cacgagcggc gcgcccggcc tcgcgccggc
    31321 cgccgcggaa gctgcgggcg gcgccgccgc ccgtggctcg ccgccgcgcg ccagcctcca
    31381 cacggcgagc gcgagcgcaa ggttgagcgc gcccgccaac tgcagggtcc cgggcaagcc
    31441 gagccagccg aacagccaga acgcggcggc cagcgcgccc gccgccgcgc cgatgcagtt
    31501 gctgaagtaa agcagcgcga gccggcgccc gccggccggc tggccgccgc catcgcgcgg
    31561 gaaacggcgg atgacggccc cggccatcag cgggaaggtc atgccgagca gcaccgtctg
    31621 cggcgcgatg agcagcgcgc acaccgcgta cttgtagagc tcgatcgttt ccggcgcgct
    31681 caaggcggga atcacgtgct cgagcgagag cgcggcgagc gtggtgaacg cagcgtggaa
    31741 ggaaagcgcg gccacaccga tgagcgcctc gatccagccg taggccgcga gcaggttctt
    31801 ccagcgcgcg ctcaggcggc tggcgagcca ggcgccgagc gccatgccgc ccatgaacac
    31861 ggcgagcacg aacgactgcg cgaacgccgc cgcgccaagg aacagcttca ggtagtgcga
    31921 ccagacggat tcgtagacca gcccggtgaa cccggacagc gcgaacagcg ccagcagcag
    31981 gcggggctcg gcgcgcatcc cgggcgctcg ccgcgctctc agggcttcgg cttctcgccc
    32041 gccgcctgca ggaacagcgc gctcgagtcg agcttgccga gatcggcgcg gcgtgccgcc
    32101 tggaactggc tgcgcaccag cgcggcgaac ggcaccggcc ggtcgagctc ctgcgcgagc
    32161 agctggaagt agcgcaggtc cttctccatc agctgcagct ggaactgcgg cgcgaagtcg
    32221 cggctgaaca tcttcggcat gcgcgtcttc atcaggcgcg acccggcggc gccctcgccc
    32281 agcacctcca gcgtggcggc gcgatccagc ccggcggctt cggcgatcat cacggcctcc
    32341 gcggtggcgg cagtcgccgc ggcggagagc atgttgttga tgagcttgat ggtggcgccg
    32401 gtcccggcgg cgcccatgtg cttcgccatg cggcccatgg ccgcaatcac cggctgcgcg
    32461 cgctcgaagg cgacacgctc gccgccgacc atgaagacga gctccttgcc ctgggcctgc
    32521 gccaggcttc ccgacacggg cgcatcgaga tactgcacgc gccgcgccgc ggctgcctgc
    32581 gcgacttcgc gcgccatggc gggcgtattg gtgctggaat cgatgatgat cgtgcccgcg
    32641 gtggcggcgg cgaccgcgcc gtccgcgccc agcatcacgg cgcgcgtggc cacgtcgtcg
    32701 gccaccatgg atacgacgaa ggaggcgccc tgtaccgcgg cggcgggcgt gtcgcaggcc
    32761 tgtgcgccct tgtccacaag cgcacgcgtc ttggcgcggt cgcggttgta gacccgcagc
    32821 gcgtagccgg cggattgcag gtttatcgcc atgcgcgagc ccatcgcgcc cagtccgagg
    32881 aaagcaatag tggtcgccat aggacaatcc tagcgcatcg gcgggataat ccggccatgc
    32941 cgacccccct gtggcagccc tccgaggacg cgatcgagca ggcgcagatc acgcaattcg
    33001 cgcgccatgc ggtgcgcaag tatcggctcg acctgaatac gtatgccgat ttctatcagt
    33061 ggacggtgga caacccggag ctcttctgga acgaggtgtg ggaatggtgc ggcgtgaagg
    33121 cgtcgcgcaa ggggtcgacc gtactggtcg atggcgacaa gatgcccggc gccaagtggt
    33181 ttcccgaggc gcgcctcaat ttcgccgaga acttgctgcg gcgcggcgac cgcggcgacg
    33241 ccttcgtgtt ctgggacgag cgcggcccgc agcgccgcgt cagctactcc gacctgacga
    33301 gcgacgtatc gcgcgccgtg caggcgctgg cgcagctcgg gctgcgcgcc ggggaccgcg
    33361 ccgcggcgtt catccccaat ctgccggaca ccgcgatgct tgcgctcgcc gcgctgtcgc
    33421 agggcatcgt ctggtcgtcg tgctcgccgg atttcggtgt cgacggcgtc atcgaccgct
    33481 tcgggcagat cgagcccaag gtgctgttcg tcgccgacgg ctacatctac aacggcaagg
    33541 agcaccgcgt gctcgagcgc gtgcgcgaga tcgccgagcg cttgccgtcg ctgcgaaaag
    33601 tggtcgtggt gccctacctc gacgcgcggc ccgacatcga ggacgtcccc aaggccgtgc
    33661 tgctggagga gtggctgcgc cggcacacgc cgggcgacat cgcctgggcg cagctgccgt
    33721 tcgaccatcc cgtgtacatc ctgttcacct cgggcaccac cggcaagccc aagtgcatcg
    33781 tgcacggcgc gggcggcaca ctgctcgccg cgctgaagat gtacaagctg cagttcgacg
    33841 tgcgtcccgc ggaccacttc ttctactaca ccacctgcaa ctgggtgatg tggaacctgc
    33901 tgttccacgg cctcgccgcg gaagcgacgg tgatgctcta cgacggctcg ccgttcgcgc
    33961 gcgacggcaa gatcctcttc gactacgcgc agcgcgagcg cttcagccat ttcggcacct
    34021 cggcgaagtt catcgacgcc atcgccaagc gcggcctgaa gccgcgcgag gcctacccgc
    34081 tgccgggact gcgcatgatc gtgtccaccg gctcgccgct ggtggccgag agctacgact
    34141 acgtctaccg cgacatcaag gccgatgtct gcctgtcgtc gatctcgggg ggcacggaca
    34201 tcatggcggg attcgcggac gccaacgccg cgctgccggt gtaccgcggc gagctgcagt
    34261 gccgctcgct cggc
//
LOCUS       DBNHKE010000051         6370 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_483514, whole genome shotgun sequence.
ACCESSION   DBNHKE010000051 DBNHKE010000000
VERSION     DBNHKE010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_483514"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1481
                     /gene="argH"
                     /locus_tag="AC16O9_03090"
     CDS             54..1481
                     /gene="argH"
                     /locus_tag="AC16O9_03090"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6515634.1"
                     /translation="MAPPRTKRKSRPRAGAGRPAAPWSPRFAEPLDDLVKRFTASVAF
                     DRRLAAVDIQASLAHARMLAACGIIARDDLADIERGMAAIRKEIEAGEFAWSLDAEDV
                     HTNIERRLIERVGEAGKRLHTARSRNDQVATDVRLWLRAEIDAMDGAIGRLQEALLVQ
                     AERHAALVMPGFTHLQVAQPVTFGHHLMAYYEMLARDRARFAECRRRVNVLPLGAAAL
                     AGTTFAIDRARVARELGFESVAANSLDAVSDRDFAIEFCADAALAMTHLSRLAEELVL
                     WMSPRFGFVRLPDRFCTGSSIMPQKKNPDVPELVRGKSARVAGSLMTLLMLMKAQPLA
                     YNKDNQEDKEPLFDAADTLRDALTVFAELVAGLEPVPEAMRAAALEGFATATDLADYL
                     VRKGVPFRDAHSVVARAVREAESVGCDLSGLPLAVLKRFSSVIGDDVYECLSLEGSVA
                     ARDHPGGTAPRQVRAAVARARRGKA"
     gene            1478..2776
                     /locus_tag="AC16O9_03095"
     CDS             1478..2776
                     /locus_tag="AC16O9_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6515635.1"
                     /translation="MNEAVVAMPERIGKYPVLQEIGRGATSVVYLCNDPFYSRNVAVK
                     VVIPDVSQNKDKGRLFRKLFITEASLAGKLQHPHICQIYDAVANDREQYIVMEYVDGG
                     TLERHGDAQDLLPIDRVVEIVFKCTRALEFAHKMGITHRDLKPANILYTGETDVKVSD
                     FGAALIASGDITQVSAIGSPAYMSPEQVKEHALDHRTDIYSLGVVMYQLLAGRLPFQA
                     TNNFSLIYQIANVEPQPPSSYRPQIPAALDAIVKRAMAKDLAQRYANWEDFSLDLAEV
                     FRNERLSGKREQFADSDKFESLRRLAFFINFTDAELWEVARISNWRTAAAGELLMKEG
                     EPGNFFCILADGQVRVTRKGKLLNILSAGECFGEMAYLSKEEQLRGADVTVMEESKII
                     SVPTQKLTQASDACRYKFDRAFMEILVERLTMANARLSGV"
     gene            2981..3457
                     /locus_tag="AC16O9_03100"
     CDS             2981..3457
                     /locus_tag="AC16O9_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR6515636.1"
                     /translation="MKPFALGIAALAAFSVSAPSAATDVTYRKDIRPLMEQKCLGCHG
                     SGAPYYGDFKEDVKKYEAAMKGPRMDTYADTIYFVGWPDTGALMRRLDDGKASKSGKP
                     GNMYAYLGSSDEERQKNFKLFKGWVGEDAWVLNRWKARGDVPGITQDQVSKMKLKY"
     gene            complement(3729..4052)
                     /gene="grxD"
                     /locus_tag="AC16O9_03105"
     CDS             complement(3729..4052)
                     /gene="grxD"
                     /locus_tag="AC16O9_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR6515637.1"
                     /translation="MDTQQRIKEQVASNPVVLFMKGTPQMPQCGFSALAVQALQACGV
                     SSFASVDVLADPEIRQGIKEYANWPTIPQLYVNGEFVGGADILREMYQSGELQKLLEG
                     VKQQA"
     gene            complement(4055..4849)
                     /gene="prmC"
                     /locus_tag="AC16O9_03110"
     CDS             complement(4055..4849)
                     /gene="prmC"
                     /locus_tag="AC16O9_03110"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6515638.1"
                     /translation="MKVAQALAALEAREARLLLAAATGYSEASVLAHPERELPAEAEA
                     KFRDYAARRARGEPIAYILGVKEFYGLPLAVNPAVLIPRPETELLVELALGLAPASVL
                     DLGTGSGAIALALKRHQPAARVVAVERSAAALAVAQRNALRLGLEVEFRHGLWYEPVA
                     GERFDAVVSNPPYVSEDDPHLAEGDVRFEPRSALAAGPDGLASIREIAGGAAAHLRPG
                     GRLLLEHGLGQEAEVRKLLREAGLEDVRSWPDLAGIARVSGGGLES"
     gene            complement(4846..5931)
                     /gene="prfA"
                     /locus_tag="AC16O9_03115"
     CDS             complement(4846..5931)
                     /gene="prfA"
                     /locus_tag="AC16O9_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6515639.1"
                     /translation="MKPSLRAKLERTLVRLEELNRLLAAEDATRDLGQFKNLSREYAE
                     ASGLAALYARYRQAEADAQAAQDMAGDAAMKGFADEELKGARAQMQRLEDELQKQLLP
                     RDPNDERSIFLEVRAGTGGDESALFAGDLLRMYTRYAERQGWPVELISESPSELGGYK
                     EVIVRIGAQGAYSRLKFESGAHRVQRVPATEAQGRIHTSACTVAVLPEAEALDDVVLN
                     PAELRIDTFRASGAGGQHVNKTESAIRVTHLPTGIVVECQDSRSQHKNREKALGVLAA
                     RIKDKQTREQEAKTASARKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDRIMD
                     GELGELLDALAAEHQAEQLAQLAEEAA"
     gene            complement(5928..>6370)
                     /locus_tag="AC16O9_03120"
     CDS             complement(5928..>6370)
                     /locus_tag="AC16O9_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6515640.1"
                     /translation="PMFMVDLAVPRDIEVEVAELNDVFLYTVDDLHAIVQGNLDARRS
                     ALEQAEAIIETQVGQFMHWMAARASVPLIRQLRDQGEAARQHELERALRHLQRGDDPK
                     AVLEALSQGLTNKLLHAPTQALNEAAGDERQALSALIERLYRSR"
BASE COUNT         1007 a         2225 c         2140 g          998 t
ORIGIN      
        1 ttttgcttta tactcattgg catagcgcgg cgcgccgcga ccaagtatgc cccatggccc
       61 ctcctcgcac caagcggaag tcgcgcccta gagccggggc ggggcgtccc gcggcgccct
      121 ggtcgccgcg cttcgccgag ccgctcgacg acctggtgaa gcgcttcacc gcctcggtgg
      181 ccttcgaccg gaggctcgcg gcggtcgata tccaggcgtc gctcgcgcat gcgcgcatgc
      241 tcgccgcctg cggcatcatc gcccgggacg accttgccga catcgagcgc ggcatggcgg
      301 cgatccgcaa ggagatcgag gcgggcgaat tcgcctggtc gctcgacgcc gaggacgtgc
      361 acaccaacat cgagcgccgc ctgatcgagc gggtcggcga ggccggcaag cgcctgcaca
      421 ccgcgcgctc gagaaacgac caggtggcga ccgacgtgcg cctgtggctg cgcgccgaga
      481 tcgacgccat ggacggcgcg atcggccggc tgcaggaggc gctgctcgtg caggccgagc
      541 gccacgcggc gctggtgatg ccgggcttca cgcacctgca ggtggcgcag ccggtgacct
      601 tcggccatca cctgatggcc tactacgaga tgctcgcgcg cgaccgcgcg cgcttcgccg
      661 agtgccgccg gcgcgtcaat gtcctgccgc tgggcgccgc ggcgctcgcc ggcaccacct
      721 ttgccatcga ccgcgcgcgc gtggcgcgcg agctcggctt cgagagcgtc gccgccaact
      781 cgctcgacgc ggtgtccgat cgcgacttcg ccatcgagtt ctgcgcggat gccgccctcg
      841 ccatgacgca cctgtcgcgc ctcgccgagg agctggtgct gtggatgagc ccgcgcttcg
      901 gcttcgtgcg cctgcccgac cgcttctgca ccggcagctc gatcatgccg cagaagaaga
      961 accccgacgt acccgagctg gtgcggggca agagcgcgcg cgtcgccggc agcctgatga
     1021 cgttgctgat gctgatgaag gcgcagccgc tcgcctacaa caaggacaac caggaagata
     1081 aggagccgct gttcgacgcc gcggacacgc tgcgcgacgc gctcaccgtg ttcgccgagc
     1141 tggtggcggg cctcgagccg gtgcccgagg cgatgcgcgc ggcggcgctc gagggcttcg
     1201 ccaccgccac cgacctcgcc gactatctgg tgagaaaggg cgtgccgttt cgcgacgcgc
     1261 acagcgtggt ggcgcgcgcg gtgcgcgagg ccgaaagcgt gggctgcgat ctctcgggcc
     1321 tgccgctcgc ggtgctcaag cgcttctcga gcgtgatcgg cgacgacgtg tacgagtgcc
     1381 tgtcgctgga gggctcggtg gcagcgcgcg accaccccgg cggcaccgcg ccgcgccagg
     1441 tcagggcggc ggtggcgcgc gcgcggcgcg gcaaggcatg aacgaggccg tggtggcgat
     1501 gcccgagcgc atcggcaagt acccggtgct gcaggagatc gggcgcggcg cgacgagcgt
     1561 ggtgtacctg tgcaatgacc cgttctacag ccgcaacgtc gccgtcaagg tggtgatccc
     1621 ggacgtgtcc cagaacaagg acaaggggcg cctgttccgc aagctcttca tcaccgaggc
     1681 ctcgctcgcc ggcaagctcc agcacccgca catctgccag atctacgacg cggtcgccaa
     1741 cgaccgcgag cagtacatcg tcatggagta cgtcgacggc ggcacgctcg agcgccacgg
     1801 cgacgcgcag gacctgctgc cgatcgaccg cgtggtggag atcgtgttca agtgcacgcg
     1861 cgcgctggaa ttcgcgcaca agatgggcat cacgcaccgc gatctgaagc ccgccaacat
     1921 cctgtacacg ggcgagaccg acgtcaaggt ctcggacttc ggcgccgcgc tcatcgcgag
     1981 cggcgacatc acgcaggtct cggccatcgg ctcgccggcc tacatgtcgc ccgagcaggt
     2041 caaggagcat gcgctcgacc accgcaccga catctactcg ctcggcgtgg tcatgtacca
     2101 gctgctcgcc gggcgcctgc cgttccaggc caccaacaac ttcagcctga tctaccagat
     2161 cgccaacgtc gagccgcagc cgccctcgtc ctaccggccg cagatcccgg cggcgctcga
     2221 cgcgatcgtg aagcgcgcca tggccaagga cctggcgcag cgctacgcga actgggagga
     2281 cttctcgctc gacctggcgg aggtgttccg caacgagcgc ctcagcggca agcgcgagca
     2341 gttcgccgat tccgacaagt tcgagtcgct gcgaaggctc gccttcttca tcaacttcac
     2401 cgacgccgag ctctgggaag tggcgcgcat ctccaactgg cgcaccgccg ccgccggcga
     2461 gctgctgatg aaggagggcg agcccgggaa cttcttctgc atcctcgccg acggccaggt
     2521 gcgcgtgacg cgcaagggca agctgctcaa catcctgtcc gcgggcgagt gcttcggcga
     2581 gatggcctac ctgtccaagg aggagcagct gcgcggggcc gacgtcacgg tgatggagga
     2641 atcgaagatc atctccgtgc cgacgcaaaa gctcacgcag gcgtccgacg cctgccgcta
     2701 caagttcgac cgcgccttca tggagatcct ggtcgagcgc ctgaccatgg ccaacgcccg
     2761 gctctcgggc gtgtgagcgc gatgcgccgc cactgatttt cttgtccgta tgctggcgtg
     2821 atttccatcg tgacccgacg caagcctaat ctgctcaggg aggatggcgg agttgcctca
     2881 accttaacct cggtcaaaat cgggccccgg atgttctggt agatgtctcc gagtaacacg
     2941 agaactagaa gcagtccaac caagctgcag gagaaaatca atgaagccat ttgctctggg
     3001 aatcgcggcc ctggcggcct tttccgtcag cgccccgtcc gccgctacag acgtgaccta
     3061 tcgaaaggat atccgaccgc tgatggagca gaaatgcctt ggctgccacg gctccggcgc
     3121 accgtactat ggcgacttca aggaagacgt gaagaaatac gaggcggcca tgaaaggccc
     3181 gcggatggat acctatgccg atacgatcta cttcgtcggc tggccggata cgggcgcatt
     3241 gatgcgcaga ctcgacgacg gcaaggcaag caagagcggc aagccgggca acatgtatgc
     3301 ctacctcggt tcgagcgacg aggagcggca aaagaatttc aagctcttca agggctgggt
     3361 aggcgaggac gcgtgggtac tcaaccgctg gaaggcgaga ggcgacgttc ccggcataac
     3421 gcaggatcaa gtctcgaaga tgaagctcaa gtattagcga acccggaaca atcgcggcac
     3481 ttacccgccc cacttgttgg ggcgttttct tgccggccaa caaacaagac aatccatccg
     3541 ctcctcgcga agcggattca ggcgatgtcc gggccggggt tgtaggtcag gatctgctct
     3601 gacacctcag gcggcttcgg ggccggtagc ccaggccccg ttcccgccgg agtccttggc
     3661 gcccacaggc caaggtggag cgctcgagcg cccctttgca agcagcccgt aggtacgcag
     3721 accgcgatct aggcctgctg cttcacccct tcgagcagct tctgcagctc gcccgactgg
     3781 tacatctcgc gcaggatgtc cgcgccgccg acgaattcgc cgttgacgta gagctgcggg
     3841 atggtgggcc agttggcgta ttccttgatg ccctggcgga tctcgggatc ggcgagcacg
     3901 tcgacgctgg cgaagctcga gacgccgcag gcctgcagcg cctgcaccgc gagggccgag
     3961 aagccgcact gcggcatctg cggcgtgccc ttcatgaaaa gcaccaccgg gttggaggcg
     4021 acctgctctt tgatgcgctg ttgggtgtcc atagtcacga ttctagtccg ccgcccgaga
     4081 cccgggcaat gcctgcaaga tcgggccagc tgcgcacgtc ttcaagaccc gcctcgcgca
     4141 gcagctttct gacctccgcc tcctgcccca ggccgtgctc gagcagcagg cgcccgcccg
     4201 ggcggaggtg cgccgccgcg ccgcccgcga tctcccggat cgacgccagg ccgtcgggcc
     4261 ccgcggccag cgccgagcgc ggctcgaagc gcacgtcgcc ttcggcgagg tgcggatcat
     4321 cctcactcac gtaaggggga ttcgagacca ccgcgtcgaa acgctcgccc gccaccggct
     4381 cgtaccacag gccgtggcgg aactcgacct ccaggccgag ccggagggcg ttccgctgcg
     4441 ccacggcgag cgccgcggcg ctcctttcca ccgccaccac cctggccgcc ggctgatggc
     4501 gcttcagcgc cagcgcaatc gcgccgctgc cggtgcccag atcgagcacg ctcgcgggcg
     4561 ccagcccgag cgcgagctcc accagcagct cggtttcggg acgcggaatg agcaccgccg
     4621 gattgaccgc gagcggcaag ccgtagaact ccttcacgcc gaggatgtag gcgataggtt
     4681 cgccgcgcgc ccgccgcgcc gcgtagtcgc ggaacttcgc ctcggcctcg gcgggcagct
     4741 cgcgctccgg atgcgcgagc acgctcgcct ccgaataccc cgttgccgcg gcgagcagca
     4801 agcgcgcctc gcgcgcctcg agcgccgcga gggcctgcgc caccttcaag cggcttcttc
     4861 ggcgagctgc gccagctgct cggcctgatg ctccgccgcg agcgcgtcga gcagctcgcc
     4921 cagctccccg tccatgatgc ggtcgatctt gtacagcgtc aggttgatgc ggtggtcggt
     4981 gacgcggccc tgcgggaagt tgtaggtgcg gatgcgctcc gagcggtcgc ccgagccgac
     5041 gagcgacttc cgcgccgagg cggtcttcgc ctcctgctcg cgcgtctgct tgtccttgat
     5101 gcgcgcggcg agcacgccca gcgccttttc gcggttcttg tgctgcgagc gcgagtcctg
     5161 gcactcgacc acgatgccgg tcggcaggtg cgtgacgcgg atcgccgact cggtcttgtt
     5221 gacgtgctgg ccgcccgcgc ccgaggcgcg gaaggtgtcg atgcgcagct ccgcgggatt
     5281 cagcaccacg tcgtcgagcg cctcggcctc gggcagcacc gccaccgtgc aggccgaggt
     5341 gtggatgcgc ccttgcgcct cggtcgcggg cacgcgctgc acgcggtgcg cgcccgactc
     5401 gaacttcagg cgcgaatacg ccccttgcgc gccgatgcgc acgatcacct ccttgtagcc
     5461 gccgagctcg gagggactct cggaaatgag ctccaccggc cagccctggc gctcggcata
     5521 gcgtgtgtac atgcgcagca ggtcgcccgc gaacagggcc gactcgtcgc cgccggtgcc
     5581 ggcgcgcacc tcgaggaaga tgctgcgctc gtcgttcggg tccctgggca gcagctgctt
     5641 ctgcagctcg tcctccagcc gctgcatctg ggcgcgcgcg cccttcagct cttcgtcggc
     5701 gaagcccttc atggccgcgt cgccggccat gtcctgcgcg gcctgcgcgt cggcctcggc
     5761 ctggcggtag cgcgcgtaca gcgcggcgag ccccgaagcc tcggcatact cgcgcgacag
     5821 gttcttgaac tggccgaggt cgcgcgtcgc gtcctcggcg gcgagcaggc ggttcaactc
     5881 ctccaagcgc acgagcgtgc gctcgagctt ggcgcgcagc gacggcttca ccgggagcgg
     5941 tacaggcgtt cgatgagcgc cgagagcgcc tggcgctcgt cgccggcggc ctcgttcagc
     6001 gcctgcgtcg gcgcgtgcag cagcttgttg gtgaggccct gcgacagcgc ttcgagcacc
     6061 gccttcgggt cgtcgccgcg ctgcaggtgc ctgagcgcgc gctcgagctc gtgctggcgc
     6121 gccgcctccc cctggtcgcg cagctggcgg atcaacggca ccgaggcgcg cgcggccatc
     6181 cagtgcatga actgcccgac ctgcgtctcg atgatggcct cggcctgctc cagcgccgag
     6241 cggcgcgcgt cgaggttgcc ctgcacgatg gcgtgcaggt cgtcgaccgt gtagaggaac
     6301 acgtcgttga gctccgccac ctcgacctcg atgtcgcgcg gcacggcgag gtccaccatg
     6361 aacatggggc
//
LOCUS       DBNHKE010000052        26998 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_801429, whole genome shotgun sequence.
ACCESSION   DBNHKE010000052 DBNHKE010000000
VERSION     DBNHKE010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 26998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 26998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_801429"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="AC16O9_03125"
     CDS             complement(<1..1239)
                     /locus_tag="AC16O9_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR6515641.1"
                     /translation="MIPELGQLALSLALVVALVQGTLPLAGAARGDAGWMGVARPAAQ
                     AQALLVAFAFGCLVYSFAVNDFSVQYVAQNSNSALPLHYRISGAWGGHEGSFLLWCSM
                     LGLWMFAVTLFSAHLPEEMTARVLGVLGLVSAGFLAFMLFTSNPFDRLLPAAADGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAYAFAVAALLSGRLDAAWARWSRPWTTVAWAFL
                     TLGIALGSGWAYYELGWGGWWFWDPVENASFMPWLIGTALIHSLAVTEKRGAFRSWTV
                     LLAIVAFGLSLLGTFLVRSGVLTSVHAFAVDPARGVFILGLFAFAIGGPLVLYAWRTA
                     RIGLGGEFAPLSRESLLLANNVLLAAAAGAVMLGTLYPLALDALSGGKISVGPPYFDT
                     VFAPLMAPALFLMGVGPLARG"
     gene            complement(1236..1670)
                     /gene="ccmE"
                     /locus_tag="AC16O9_03130"
     CDS             complement(1236..1670)
                     /gene="ccmE"
                     /locus_tag="AC16O9_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR6515642.1"
                     /translation="MKPRHKRLAAIAVGLAALGVAAALVLSAFEKNLVFFFTPSQVAA
                     NEAPQGKTFRIGGMVETGSVKREGVEVRFLVTDTARSIPVVYSGALPDLFREGKGVVA
                     QGALGADGVFRAREVLAKHDENYMPPEAAHALEQAQKTLQVK"
     gene            complement(1654..1794)
                     /gene="ccmD"
                     /locus_tag="AC16O9_03135"
     CDS             complement(1654..1794)
                     /gene="ccmD"
                     /locus_tag="AC16O9_03135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR6515643.1"
                     /translation="MNWGGWSEFWAMGGYGFYVWGSYAATLAGLAMEVVLLRRRAREA
                     KT"
     gene            complement(1791..2495)
                     /gene="ccmC"
                     /locus_tag="AC16O9_03140"
     CDS             complement(1791..2495)
                     /gene="ccmC"
                     /locus_tag="AC16O9_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HIR6515644.1"
                     /translation="MSAAIKWYWFASPQTFFPLAGTLARWSGALAALLAAAGLYVGFF
                     VAPTDAQQGEAYRIIFIHVPAAWMSMFLYVVMAFWAAIGLAFNTRLSAMMASAIAPTG
                     ALFTFLALWTGSLWGRPTWGAYWVWDARLTSELILLFLYLGFMALQAAIDEPRRADRA
                     GAVLAIVGVVNVPIIYYSVQWWNTLHQGASVSLTRAPSMASTMLTGMLLMALAFWAYS
                     IATTLARVRCIIKERE"
     gene            complement(2492..3160)
                     /gene="ccmB"
                     /locus_tag="AC16O9_03145"
     CDS             complement(2492..3160)
                     /gene="ccmB"
                     /locus_tag="AC16O9_03145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR6515645.1"
                     /translation="MLDAARTIVTRDLLLALRRPSDVGTALLFFVIVASLFPLGIGAE
                     PNLLRLVAPGVIWVAALLSSMLSLARLFAADHADGTLEQLLLGATPLGVVASAKVLAH
                     WLVSGLPLVVIAPVIALQYDLPQSLYGTLALSLLLGTPVLSLIGAIGAALTLGLRGGG
                     VLLSLLVLPLYVPVLIMGAGSVDMAAAGLSPQGQLQLLAALLVVAAAFAPWAIAAALR
                     ISTE"
     gene            complement(3162..3773)
                     /gene="ccmA"
                     /locus_tag="AC16O9_03150"
     CDS             complement(3162..3773)
                     /gene="ccmA"
                     /locus_tag="AC16O9_03150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HIR6515646.1"
                     /translation="MLDASNLDCQRGGRTLFRGLSFELRAGELLRIAGANGSGKTSLL
                     RILCGLLAPQAGEVRWNGAPIQRLREEYARHIVYLGHAPAVKDDLSTEENLSIACRLA
                     GMPVPGAAIRDALARFGLAGAALVKKLSQGQRRRAALARLWLSASVPLWLLDEPFTAL
                     DTAGVAMLRGILEGHAGRGGAVVYSTHQDPGMAATRVVELVIA"
     gene            4238..6439
                     /locus_tag="AC16O9_03155"
     CDS             4238..6439
                     /locus_tag="AC16O9_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR6515647.1"
                     /translation="MNKVSLLLAALAMTLAGAAGAQSGLEQLSAKGCLGCHDMSAAKV
                     GPSFQEIAAKYRKDKDALGKLSAALKEGTGHPVKAEASAAELKAMLQHILGAKSPAAA
                     KAAEPAVATAAQPAAAALDNATCLGCHGIDGFSMPDADGKPRNLHVVQEKFAASVHGK
                     RQCVDCHRDITEIPHQKVAPLKVSCVQCHEDLWQAMQDGKPGAAQNPRLSVVVDQIER
                     YMKSVHARPRRDDQSRTNATCHNCHEAHYVYPKDSPARAEWRLSIPDRCGACHAKQRA
                     DYATSVHGKEVLENKNLYAAVCSDCHSTHDIDRPEGDPIKLAITRNCGNCHAESLRTY
                     TETYHGQVSTLGYAHTAKCFDCHDSHAIQRVSEPESTVHPARRLETCRKCHAGVGAGF
                     VTFEPHATSDDFARYPHSWIATKFMIALLVGVFVFFWTHTALWFYREYRDRKEGRTRP
                     HVMLDESMQLQGKQIQRFGPVWRLAHLVFALSVMTLVLTGMTVLFPGSDWAKSVAGAF
                     GGPKVAALVHRTAAAIMLGIFFVHLVYLLARIARDWRTFRWFGPTSLVPTWQDLKDIG
                     AMFAWFVGRRPRPAFDRWTYWEKFDYWAVFWGMAIIGGSGMMLAFPGATASVLPGWAL
                     NVATIVHGEEAILAAVFLFTVHFFNNHFRPDKFPLDTVMFTGAVPLEEFRREHALEYQ
                     RLAAAGELERHLVDAPSSAMTLGSRILGFALIAIGLVLLVLVIIGFAGRTG"
     gene            6469..6756
                     /locus_tag="AC16O9_03160"
     CDS             6469..6756
                     /locus_tag="AC16O9_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515648.1"
                     /translation="MKAKLFVLAMTGVLAASSAGLVAAADVLKAKGCLNCHDAEKKKV
                     GPSFNDIAAKFKGKQGAAGDLVAKLRDGKGHMKIKASDAELEAAVKQALGQ"
     gene            6853..7833
                     /locus_tag="AC16O9_03165"
     CDS             6853..7833
                     /locus_tag="AC16O9_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515649.1"
                     /translation="MPKHIVRLIALLAAGLALGLAAKWYFTADSFYEYGHYRAKSVPE
                     IAAQVPAYQSAAYCQGCHVERHAQWSANNHKSVTCEVCHGAAQGHPQNGKLPIPADAR
                     RLCTLCHEKMAERPRTQPQIVSAEHGAGQQCVVCHNPHAPKLALAAALVAGDAAAGGR
                     KHAESCASCHGENGISPNDTWPSLAGQNAAYLARILGAYKSGAQSDVMMSPVAQVLSD
                     ADVQNLAAYYAGLSCRAPARAAGAAAAAGKLLAKNCEACHGETGITANPAWPHIAGQK
                     STYLANTLKAFRAGLRKDPMMAGVVRGLSDADIANLAAYYAAQRCAPGPQ"
     gene            7845..8690
                     /locus_tag="AC16O9_03170"
     CDS             7845..8690
                     /locus_tag="AC16O9_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6515650.1"
                     /translation="MKRDSISAMRARRGFLRGIGRAAAVAAAAATAGTGALRQADAKS
                     APGAGQAAPPIPDYDWNQHSWAFGVDATKCIGCLRCVEACKHENDVPLTAHHFRTWVE
                     RYVYLEGEEHPRIDSQQDPVNIAAAGSEREYRFADRYAGAKVEKAFFVPKLCNQCTHP
                     ACVQVCPVGATYKGADGVVLIDHDYCIGCRYCVQACPYGARYFDERKGVSDKCTWCYH
                     RITKGQQPACVEVCPVGARIFGDRRDRQSPISLFIRNNRVQVLKPETGNEPNVYYVGI
                     DKAVL"
     gene            8690..9862
                     /gene="nrfD"
                     /locus_tag="AC16O9_03175"
     CDS             8690..9862
                     /gene="nrfD"
                     /locus_tag="AC16O9_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6515651.1"
                     /translation="MEPLIHYAPYIGWVYPNETVIEWSIAIVVYPYITGLVAGAFTVS
                     SLYQVFGFQRLKPVAHLALLTSLCFMIFVPLPLLLHLGHPERALNNLLTPHWTSAMAM
                     FGYFATFYIVLLMVETWFAFRPYIVEQAQKRKGVLGLAYRLLTLGSYDVSEEALSHDR
                     KWIFWLAIAGIPGAHGLHGYVGFVFGSLKSREWWSSDLMPVVFLFSAIISGVALLIVI
                     YVASCWLRRIRIDLACLKGLAYTVWGFIMVALVLEGLEFANIVYKGREGVEMIIAYVN
                     GPLLIPYFVLQFAVGAILPLLWLSYMIWRGTTGKAFITGVTLCACLVLLSVFMMRWNV
                     VIGGQEISKTGRGLLGYQLQLLGHEGLLVAILLLLAPIATLVGLTRFFPPWEDQPG"
     gene            9859..11091
                     /locus_tag="AC16O9_03180"
     CDS             9859..11091
                     /locus_tag="AC16O9_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515652.1"
                     /translation="MRMLVASVLVVGAAATAYASGAAAQAVRVPAGSDDQLRAVYATP
                     LEIAEGGRIAQTACARCHGANGVSTTKGVPHLAGQRAAYIDLQLRAYRHSARVQGAMT
                     GVVRYLSDDALVKVAAYYGSVEPPRPAPAPAKAAPKAADPLQAGKAAAAGCGGCHGES
                     GVSAIAGMPSLVGLDPKYFVAAVNAYKSGARKHDLMKSLVAAMSAETVSQVALYYALQ
                     KPAKAQTPAPGDAAAGKAAAASCAACHGDTGVSGNPITPSIAGQDAEYFVVALRAYKD
                     GARKDDSMNGPAAALDEKAMRDLAAYYAAQQPQAPNVRRPLTAAEWIERCDRCHGANG
                     NSTDPLMPRLSAQREDWLEQVLDAYRTGVRKSTAMAAMSALMSERDVKELAAHYSRQG
                     ARAVVYLVLPPADKKSEK"
     gene            11088..11891
                     /locus_tag="AC16O9_03185"
     CDS             11088..11891
                     /locus_tag="AC16O9_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515653.1"
                     /translation="MSKETRMDDAPLHRLYRHFSELPYSLRVLYTATLLVLGTAYLFA
                     MIYLFHTYSGKDGNPRSVSYEDIVIAYSGSGKGSRLEAALRGSMSAMLPRDELTSLVS
                     WVQEGGERGRYEKEIKTVLDGRCLTCHDGSNPHLPNLNGYDNVLKVTERDTGTGIFTL
                     VRVSHIHFFGLAFVFFILGTIFSHAYVRPVWFKCAVVGLPFVSLTLDIGSWYFTKIYH
                     PFAWVVMIGGALMGLSFAFMWVVSMYQMWFSHPPKMVTYRQGGETTDVG"
     gene            11916..13064
                     /locus_tag="AC16O9_03190"
     CDS             11916..13064
                     /locus_tag="AC16O9_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515654.1"
                     /translation="MKRHTFVLVIALSAALAGCPGKEQGTAKPAVEPLSATDLAAGRA
                     IAERDCKGCHGLDGRGAAPGIPHLAAQRVGYLVAALDAYKAGKRTHAALREIATHLGD
                     AEVRNVAGFYTSLPPVRSTAAVAQSAHASPYELGKKVAAACAKCHGEDGNSTTPGIPS
                     LAGQQPHYLVVAIQEYLLNERKAAPMHALLPRLSKLDMETLALYLASQTPAPRSAPPF
                     GDPAAGEPLSAVCGGCHGGHGVSTDSATPSLAAQDARYLVDAIKAYRTTRKRENMRLY
                     VTGLSDKDIESIAAFYAVQKSRAAEKGQTLVKDLTDRCERCHAADVDIPGMVVPKIRG
                     QDRDYLVMALRAYRDDRRESSMMHKMSLPYSDSIIESLATAYASQPAK"
     gene            complement(13083..14117)
                     /locus_tag="AC16O9_03195"
     CDS             complement(13083..14117)
                     /locus_tag="AC16O9_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515655.1"
                     /translation="MRLNPGLLWSALLAAAACIAPQAAEAQRGERSGKQVVEATCASC
                     HGEGVGGAPKIGDSEAWKNRAAQGLTALTRHALDGIRGMPAHGGNLALTDLEIARAVT
                     YMVNRSGGRWVPPASELDLAGERSGAQIVEAQCSKCHREGLGGAPRIGDIAAWAPRLK
                     GGLDDLVRAAIHGHGGMPPRGDRADLTDAEVRNAVLYMFNPGAVAPKDAPADARAAAP
                     RYKIAGGMEVDLGIVPAETLRLLPEGSAERMLHGGVPDGAGYYHLNVSLRDARKRTPI
                     GDAQVVMRVEQVGLSGVTRTLEPYMVNQLPSYGAYFRLRWNANYRITAIIRTPGAPAP
                     VAVKFEHRVY"
     gene            complement(14134..14424)
                     /locus_tag="AC16O9_03200"
     CDS             complement(14134..14424)
                     /locus_tag="AC16O9_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515656.1"
                     /translation="MKTVLIAVAAAGLLAAGAAQAQSGADVLKAKGCLNCHDADKKKV
                     GPAYKDVAAKYKGNAGAEADLLAKLKSGKGHMKIQATDAELQAALRHVLATK"
     gene            complement(14522..14812)
                     /locus_tag="AC16O9_03205"
     CDS             complement(14522..14812)
                     /locus_tag="AC16O9_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515657.1"
                     /translation="MAKIAEERLDAVRRYLQQVFPGWALAEVWDRDREAHTFLLKKPR
                     EPVHLLKVSRTLLDDCGPRKLAGLLEGHQVAHALRKASHHRLLLSGRGLDPI"
     gene            complement(14905..15276)
                     /locus_tag="AC16O9_03210"
     CDS             complement(14905..15276)
                     /locus_tag="AC16O9_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 family protein"
                     /protein_id="HIR6515658.1"
                     /translation="MRSERNVLGGELRACSYAPLTGYFRDGSCATHGTDGVAHLVCVR
                     ATQAFLDFSVAKGNDLVTPRPELRFRGLKAGDRWCLHVLRWIEAWRAGAAPQVVLEAT
                     HENVLEYVPLAALKQCALDLN"
     gene            15392..16108
                     /locus_tag="AC16O9_03215"
     CDS             15392..16108
                     /locus_tag="AC16O9_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6515659.1"
                     /translation="MLPATTNNNDARHDRIVESVVANMAMLRDATRSQLAEIAAHARA
                     RTLQRGTVACRLGEPMPGILIVAFGLLKLSLPRSESEERVLRLVGANESFGEAPALHD
                     RPAPFDAATLSESLLVVVPRAPVLRLIERNSVVAQSLVAHLAEDCLSLLSELQALAQH
                     NGVQRLASYLVSLAEREQGADGCQVRLPASKTTVAARLSIQKETLSRMLRDLTQRGCI
                     RVEGRAITILDRERLAGLAA"
     gene            16179..16769
                     /locus_tag="AC16O9_03220"
     CDS             16179..16769
                     /locus_tag="AC16O9_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR6515660.1"
                     /translation="MFQVRIHGRGGQGVVTAAEMLSIAAFLEGRHAQAFPSFGSERTG
                     APVVAFCRIDDREIRLREPVLQPDALIIQDPTLLHQVDVFGGLAHDGYVLINTSRTFD
                     ALGVGEFARSLRAERQCTVPATELALKHLGRPLPNAALLGGFAAVSDRISLDSVAAAI
                     REKFSGKVAEGNIAAASEAYAFVRGDMQEISHAQAD"
     gene            16753..18006
                     /locus_tag="AC16O9_03225"
     CDS             16753..18006
                     /locus_tag="AC16O9_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6515661.1"
                     /translation="MLKQTEGSHAVAEAVALCRPEVICAYPISPQTHIVEALGKKVKS
                     GALARCEFINVESEFAAMSVAIGAAAAGARAYTATASQGLLYMAEAVFNASGLGLPIV
                     MTVANRAIGAPINIWNDHTDAMSQRDCGWIQLFAETNQEALDLHIQAFRLGEELSLPV
                     MVCMDGFILTHAYERVDLPSQEQVDAWLPPFEPRQVLDPAEPVSIGAMVGPEAFMEVR
                     YLAHAKQMRALELISGWAEEFRGAFGRESGGLVHTYRTEDADTIVVALGSVLGTIKDT
                     VDEMRRDGAKIGVLGVTCFRPWPHAVVRAALAQARRVVVIEKSLAVGLGGIVSLNVRT
                     AFGGSPDAVHAVIAGLGGRSITRASLGRVFAAAIGGKLEPLSFLDLDHALVDRALARE
                     REQRRSGPIAEGMLRDLGVIAARMG"
     gene            18017..19009
                     /locus_tag="AC16O9_03230"
     CDS             18017..19009
                     /locus_tag="AC16O9_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6515662.1"
                     /translation="MAFQPVKFYQTGTFTVGNRLLAPEERSVQANLRRTNSLNSGHRA
                     CQGCGEALGARYAIDAAMEATSNRLIAANATGCLEVFSTPYPETSWQIPWIHSLFGNT
                     AAVATGIAAAMRVKGRDDVRVVAQGGDGGTTDIGFGCLSGMFERNDDVLYICYDNEAY
                     MNTGVQRSSATPPAARTATTMAVGEAPGNVFGPGKNLPAIAMAHGIPYVATATVAELH
                     DLEAKVRRAMGFRGARYLHLFVPCPLGWGHAAEDTIRIARLAKETGMFPVFEALYGEV
                     ASVSKIRRKLPVEEYLRPQKRFAHLFGATPRAEDIARIQGIADRNIRRYGLVDA"
     gene            19002..20627
                     /locus_tag="AC16O9_03235"
     CDS             19002..20627
                     /locus_tag="AC16O9_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6515663.1"
                     /translation="MRKPFAITLDPGSSLANHTGTWRTLRPEYVDRLPPCNSACPAGE
                     NIQGWLYHAESGDYEAAWRLLTQDNPLPAVMGRVCYHPCETSCNRAQLDSAVGINSVE
                     RFLGDEALRQGWKFDAPAAPSGKRVLVVGAGPSGLSAAYHLARLGHAVQIHEAGPVAG
                     GMMRFGIPKYRLPRDVLEAEIRRLLELGVTLKLNAKVANILETMQGGAFDAAFLAVGA
                     HIAKRAYIPAGSAAKVLDALSVLRSMEGEEKPLLGRRVVVYGGGNTALDVARTAKRLG
                     AEDAVIVYRRTREKMPAHDSEVEEALQEGVLIKWLSTIKQAAESSITVEKMVLDAKGN
                     PQPTGELETLAADSVVLALGQDVDLSLLDGVPGLEMRDGTVQVGANMMTGHDGIFAGG
                     DMVPAERTVTVAVGHGKKAARHIDAWLRGAVYAPPPKHEPATFEQVNTWYYSDAPKTV
                     RPVLDGVRRQSTFDEVVRGLDESNALYEARRCLSCGNCFECDNCYGVCPDNAVIKLGP
                     GKRFQFNFDYCKGCGICVAECPCGAIKMVPEEI"
     gene            20624..22264
                     /locus_tag="AC16O9_03240"
     CDS             20624..22264
                     /locus_tag="AC16O9_03240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR6515664.1"
                     /translation="MNARLPAGLRGMAVLRDPLLNKGTAFTQKERARLGLRGLLPPHV
                     HTQEEQMQRSLAHLRGLPSPLEKFVNLNALHDRNEALFFRIVAEHPDEIMPLIYTPTV
                     GLACQKFGNIFQRPRGLFIGANDRGKIAQVLRNWPQRDVGIIVVSDGERILGLGDLGA
                     NGMGIPVGKLSLYTGCAGVHPRRCLPVILDVGTDNEALRNDPFYVGLRQTRLRGARYD
                     ALVEEFVTAAQKVFPGVLIQFEDFANTNAFRLLQRYRERVCTFNDDIQGTAAVVLAGL
                     YSALRVTGAALREQRILCLGAGEAASGICDLVVAALGDEGLAADAARRRCWLFDSRGL
                     VVAGRTDLAAHKRPYAHEGQRVGDFAAAVEALKPTAIIGVAAVGGTFTEKVVAAMARI
                     NPRPIVFALSNPTSQSECTAEQAYHWSGGRALFACGSPFDPVTLDGRVFVPRQGNNSY
                     IFPGVGLGVISSRARRVTDEMFLAAARTLARQTTQADLDQGSLYPALARIRDVSARIA
                     AAVARVAYARHLTSQRRPANLLAHIRAQMYDPKYPRYA"
     gene            complement(22255..23619)
                     /gene="thrC"
                     /locus_tag="AC16O9_03245"
     CDS             complement(22255..23619)
                     /gene="thrC"
                     /locus_tag="AC16O9_03245"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6515665.1"
                     /translation="MRYVSTRGGAAPQRFSDILLEGLAPDGGLYVPESFPPADLAAWR
                     GLGYPQLAAAVLAPYMDDVPALETIVARTYTRDVFGSDDIAPLKTLEPGLHLLGLSNG
                     PTLAFKDMALQLLGVLFEDVLVRRRQTLNVLGATSGDTGSAAEHALRGRRGVQVFMLS
                     PHGRMSAFQAAQMYALQDANIHNLAVEGTFDDCQDVVKQVNADAAFKARHRIGAVNSI
                     NWARIAAQVVYYFWGYFRATRSDAQRVDFAVPSGNFGNIYAGYVARRMGLPVSRLILA
                     TNENDVLDEFFRTGRYRVRRAVRATSSPSMDISRASNFERYAFELVGRDPGRIVALWA
                     QLARDGEFELPGASGFVSGKSTHEDRLATIRELQRKYGVVVDPHTADGVKVGLEQRAP
                     GVPLICLETALPVKFAATIAQALGREPPRPARFEGLEQLPQRVEVIVPDARAVKRAIE
                     ASQA"
     gene            complement(23619..24929)
                     /locus_tag="AC16O9_03250"
     CDS             complement(23619..24929)
                     /locus_tag="AC16O9_03250"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR6515666.1"
                     /translation="MRPIQVGLLGIGTVGGGTWDVLNRNAGEIRRRAGRAIRIARVAD
                     KDRNKARKLVAGKAEVTGNAWEVVRDPRIDIVVELIGGTGIARDLVLEAIAHGKHVVT
                     ANKALLAKHGNRIFAAAQKKGVMVAFEASVGGGIPIIKALREGLAANRIEWIAGIING
                     TSNFILSEMRDKGLAFRVALADAQARGYAEADPTFDIEGVDAAHKLTILSALAFGIPM
                     QFAKAYTEGVSRLTQADIGYAEELGYRIKLLGITRRAPKGIELRVHPTLVPTRRLIAN
                     VEGVMNAILVKGDAVGPTMFYGAGAGSQPTASAVVADLVDVTRLITADPGNRVPHLAF
                     QPDQLSAAPILPMGEVETSYYLRLRVQDKPGVLARVTRILADSKISIEALVQKEPPAG
                     EKRADIVMLTHLAVEKNVDAALARIERLPTVLGKVTRIRLESLL"
     gene            complement(24926..26122)
                     /gene="alaC"
                     /locus_tag="AC16O9_03255"
     CDS             complement(24926..26122)
                     /gene="alaC"
                     /locus_tag="AC16O9_03255"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR6515667.1"
                     /translation="MREFSRIKRLPPYVFNITNELKMAARRRGEDVIDLSMGNPDGPT
                     PKHIVDKLVEAAQRQDTHGYSVSKGIPRLRKAVCDWYRRRYGVEFDPDSEAIVTIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAIIAGADIRSVRMTPGVDFIAEVERAIR
                     ELIPKPKMLILGFPSNPTAKCVELDFFERVVALAREHDMMVVHDLAYADIVFDGWRAP
                     SIMQVAGARDVAVEFFTMSKSYNMAGWRVGFMVGNQDLVHALARIKSYHDYGTFTPLQ
                     VASIVALEGPQDCVEEIRLTYQRRRDVMVKGLHDIGWRVENPKASMYVWAKIPEAYAA
                     MGSIEFTKRLLGEAKIAVAPGIGFGDYGDDHVRIALIENENRLRQALRGVKEMFRKDG
                     LLRGAA"
     gene            26138..26506
                     /locus_tag="AC16O9_03260"
     CDS             26138..26506
                     /locus_tag="AC16O9_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR6515668.1"
                     /translation="MKLQASAPARVNAFTGYGDGYVLLNGARREASVIVLPERVVEWP
                     VASFAALAEADFERIAALKPEVVLLGTGARLRFPHPGLAAPLARAGIGLEVMDLKAAC
                     RTYNILAAEERVVAAALVFA"
     gene            26503..26874
                     /locus_tag="AC16O9_03265"
     CDS             26503..26874
                     /locus_tag="AC16O9_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HIR6515669.1"
                     /translation="MTPVGLALVAVAVLLGTAGQILLKAGTNAIGHFEFTLSNVLPVG
                     LRVALEPRIMGGVTCYAVGMVVWIMALSRTDVSVAYPMLSVGFALNALLAWWLLGEGV
                     NAQRLAGIAVIMLGVWLVARS"
BASE COUNT         4343 a         9447 c         9118 g         4090 t
ORIGIN      
        1 cccgcgcgcg agcggcccga cgcccatgag gaacagcgcc ggcgccatca gcggcgcgaa
       61 tacggtgtcg aagtacgggg gcccgaccga gatcttgccg ccgctgaggg cgtcgagcgc
      121 gagcggatac agcgtgccca gcatcaccgc gccggccgcc gccgcgagca gcacgttgtt
      181 cgcgagcagc agcgactcgc gcgacagggg cgcgaactcg cccccgaggc cgatgcgcgc
      241 cgtgcgccag gcgtagagca cgagcgggcc gccgatggcg aaggcgaaca ggccgaggat
      301 gaacacgccg cgcgccggat cgacggcgaa ggcgtgcacc gaggtgagca cgcccgagcg
      361 cacgaggaag gtgccgagca gcgacaggcc gaaggcgacg atggcgagca gcaccgtcca
      421 gctgcggaac gcgccgcgct tctcggtcac cgcgagcgag tggatgagcg cggtgccgat
      481 gagccagggc atgaaggagg cgttttccac cggatcccag aaccaccagc cgccccagcc
      541 gagctcgtag taggcccagc cgctgccgag cgcgatgccg agcgtgagga acgcccacgc
      601 caccgtggtc cagggccgcg accagcgcgc ccaggccgca tcgagccggc ccgagagcag
      661 cgccgctacc gcgaaggcgt aggccaccga aaagcccacg tagcccatgt agagcatcgg
      721 cgggtggatc accatgcccg ggtcctgcag cagcgggttc aggtcgcgcc cgtcggcggc
      781 cgccggcagc aggcgatcga acggattcga ggtgaacagc atgaacgcga gaaagcccgc
      841 gctcaccagc ccgagcacgc cgagcacgcg cgcggtcatt tcttccggca ggtgcgcgct
      901 gaacagcgtc accgcgaaca tccacaggcc gagcatcgag caccagagca ggaacgagcc
      961 ctcgtgaccg ccccaggcgc cggagatgcg gtagtgcagc ggcagcgcgc tgttggagtt
     1021 ctgcgccacg tactgcaccg agaagtcgtt gacggcgaaa gagtacacga ggcagccgaa
     1081 ggcgaaggcg acgagcagcg cctgcgcctg cgccgccggg cgggcgacac ccatccaccc
     1141 cgcgtcgccg cgcgccgcgc cggcgagcgg cagcgtgccc tgcaccagcg ccacgacgag
     1201 ggcgagcgag agcgccagct ggccgagttc ggggatcatt tgacctgcag cgtcttctgc
     1261 gcctgctcga gcgcgtgcgc ggcctcgggc ggcatgtagt tctcgtcgtg cttggcgagc
     1321 acttcgcgcg cgcggaacac gccgtcggcg ccgagcgcac cctgcgccac cacgcccttg
     1381 ccctcgcgga acagatccgg cagcgcgccg ctgtagacca ccgggatgct cctcgccgtg
     1441 tcggtaacca ggaagcgcac ctcgacgccc tcgcgcttga cgctgcccgt ttccaccatg
     1501 ccgccgatgc ggaaggtctt gccctgcggc gcctcgttgg ccgccacctg cgacggcgtg
     1561 aagaagaaca cgaggttctt ctcgaacgcc gagagcacca gcgccgcggc gacgccgagc
     1621 gcggcgagcc cgacggcgat ggccgccagc cgcttatgtc ttggcttcac gcgcgcgcct
     1681 cctcagaaga acgacttcca tcgccaggcc ggcgagggtc gcggcgtacg acccccagac
     1741 atagaagccg tatccgccca tcgcccagaa ctcgctccaa ccgccccagt tcattcccgc
     1801 tccttgatga tgcagcgcac gcgcgccagc gtcgtcgcga tgctgtaggc ccagaacgcc
     1861 agcgccatca gcagcatgcc cgtaagcatg gtgctcgcca tgctcggcgc gcgcgtcagg
     1921 ctgacggaag cgccctggtg cagggtgttc caccactgca ccgagtagta gatgatgggc
     1981 acgttcacca cgccgacgat tgccagcacc gcgcccgccc gatcggcgcg gcgcggctcg
     2041 tcgatcgccg cctgcaatgc catgaatcct agatacagga acagcagaat aagttccgag
     2101 gtgagtcgcg cgtcccacac ccagtaggcg ccccaggtcg gccggcccca gagcgagccc
     2161 gtccacagcg cgaggaacgt gaacagcgca ccggtgggcg cgattgcgct cgccatcatc
     2221 gccgaaagcc gcgtattgaa cgccaggccg atcgccgccc agaacgccat gacgacgtag
     2281 aggaacatcg acatccaggc cgccggcacg tggatgaaga tgatgcggta ggcctcgccc
     2341 tgctgcgcgt cggtcggcgc cacgaaaaag cccacgtaca ggccggcggc cgcgagcagc
     2401 gcggcgagcg ctccgctcca gcgcgcgagc gtcccggcga gcgggaagaa cgtctgcggc
     2461 gacgcgaacc agtaccactt tatggctgca ctcattcagt cgaaatcctc aaggccgccg
     2521 cgatcgccca gggggcaaac gccgcggcaa ccaccagcaa ggctgcaagc agctgcagct
     2581 gtccctgcgg cgacagcccc gccgccgcca tgtccacgct gcccgcgccc atgatcagca
     2641 ccggcacgta cagcggcagc accagcagcg acagcagcac accgccgccg cgcagcccca
     2701 gcgtcagggc ggcgccgatg gcgccgatca ggctcagcac cggcgtaccg aggagcaatg
     2761 ataaggcaag tgtgccgtag agcgattgag gtaggtcata ctgcaaggcg ataacgggcg
     2821 cgatcactac cagcggcaat cccgacacga gccagtgggc aagcaccttg gcgctggcga
     2881 cgacgccgag cggcgtcgcc ccgagcagga gctgctcgag cgtgccgtcg gcgtggtcgg
     2941 cggcaaagag gcgcgcgagc gacagcatcg aggagagcag cgccgccacc cagatgaccc
     3001 cgggcgccac caggcgcagc aggttcggct cggcgccgat ccccagggga aagaggctcg
     3061 ccacgatgac gaagaaaagg agcgccgtgc ccacgtcgct cggccggcgc agggcgagca
     3121 gcaggtcgcg ggtgacgatc gtgcgcgcgg cgtcgagcac gtcaggcgat gaccagctcg
     3181 acgacgcgcg ttgccgccat gcctgggtcc tggtgcgtgg aatagaccac cgcgccgccg
     3241 cgcccggcgt ggccttcgag gataccgcgc agcatcgcca cccccgccgt atcgagcgcg
     3301 gtgaagggct cgtcgagaag ccacaacggc acggacgcgc tcaaccacag gcgcgccagc
     3361 gccgcgcggc gccgctggcc ctgcgagagc ttcttcacca gcgccgcgcc ggccaagccg
     3421 aaccgcgcga gcgcatcgcg gatcgccgcc cccgggacgg gcatgcccgc cagccggcag
     3481 gcgatcgaca ggttttcctc ggtgctaagg tcgtccttca ccgccggcgc gtggccgagg
     3541 tagacgatgt ggcgcgcgta ctcctcgcgc aggcgctgga tcggcgcgcc gttccagcgc
     3601 acctcgcccg cctgcggcgc gagcaggccg cacaggatgc gcaggaggct cgtcttgccg
     3661 ctgccgttgg cgccggcgat gcgcagcagc tcgcccgcgc gcaattcgaa ggaaaggccg
     3721 cggaacagcg tgcggccacc gcgctggcaa tcgaggttcg atgcgtcgag cacccgctat
     3781 tgttgcagaa agtcgcgtcg ggccgcagtc ggtgcgacgc gccgggctga tctgcatcaa
     3841 ggccgcgcca cgcgtttgtc ccggatgact tgcatttgcg ccacgcggtc gcaatggctc
     3901 cgccggcact tgaaccggcg agaccatcct agcgagtgtc tgattggcag tccacccgca
     3961 tcccttgatc tagatcagac gcagcgcaca aaacggcggt agctttcgta ccctactatc
     4021 cctgcggggc gtgccgattg gcgtgtgtgt ttgatcccgc attgtaaccg ctccgccggc
     4081 caccgcgcgg cgggtgctct cggcctcttt tattgcgcca cagaagaacc tcccgacgct
     4141 tgtaacacac atcgcttgtg gtcgacctcc gcctgcatat tcatcgcctg acttggatca
     4201 agtgctgcga ccgacgcggc catagcatgc gcgcgccatg aacaaggtct cgttgctgct
     4261 tgccgcgctc gcaatgacgc tggccggcgc ggcgggcgcg caatccgggc tggagcagtt
     4321 gagcgcgaag ggctgcctcg gctgccacga catgagcgcg gcgaaggtcg gaccgagctt
     4381 ccaggagatc gcggccaagt acaggaagga caaggacgcg ctaggcaagc tgagcgcagc
     4441 gctcaaggaa ggcacgggcc atccggtcaa ggcggaggcg agcgccgccg agctgaaggc
     4501 gatgctgcag cacatcctgg gcgcgaaatc gccggccgca gccaaggctg cggaacctgc
     4561 cgtggccacg gccgcgcagc ccgcggcggc cgcgctcgac aacgctacct gcctcggctg
     4621 ccacggcatc gatggctttt cgatgccgga tgcggacggc aagccgcgca atctgcacgt
     4681 cgtgcaggag aaattcgccg ccagcgtgca cggcaagcgc cagtgcgtgg actgccaccg
     4741 cgacatcacc gagatcccgc accagaaggt cgcgccgctc aaggtgagct gcgtgcaatg
     4801 ccacgaggac ctgtggcagg cgatgcagga cggcaagccc ggggcggcgc aaaacccgcg
     4861 gctcagcgtg gtggtggacc agatcgagcg ctacatgaaa tccgtgcacg cgcggccgcg
     4921 tcgcgacgac cagtcgcgca ccaacgcgac ctgccacaac tgccacgaag cccactacgt
     4981 gtatccgaaa gacagcccgg cgcgcgccga gtggcgcctg agcattccgg accggtgcgg
     5041 cgcgtgccac gcgaagcagc gcgcggacta cgccacctcg gtgcacggca aggaggtgct
     5101 ggagaacaag aacctgtacg cggccgtgtg ctcggactgc cactcgaccc acgatatcga
     5161 caggccggaa ggcgacccga tcaagctcgc gatcacccgc aactgcggca actgccatgc
     5221 ggagagcctg cgcacctaca ccgagaccta ccacggccag gtgagcaccc tgggctacgc
     5281 gcacacggcc aagtgctttg attgccacga tagccatgcc atccagcgcg tgagcgagcc
     5341 ggagtcgacc gttcacccgg cccggcgcct ggagacctgc cgcaagtgcc acgccggcgt
     5401 gggcgcggga ttcgtgacct tcgagccgca cgccaccagc gacgatttcg cgcgctaccc
     5461 gcactcgtgg atcgcgacca agttcatgat cgcgctgctg gtcggggtgt tcgtgttctt
     5521 ctggacgcac accgcgctgt ggttctaccg cgagtaccgc gaccgcaagg aaggcaggac
     5581 ccggccccac gtcatgctgg acgagtcgat gcagctgcag ggcaagcaga tccagcgctt
     5641 cggcccggtc tggcgcctgg cgcacctggt gttcgcgctg agcgtcatga cgctggtgct
     5701 taccggcatg accgtgctgt tccccggcag cgactgggcc aagtccgtcg cgggcgcgtt
     5761 cggcggcccg aaggtcgcgg cgctggtgca tcgcaccgcc gcggcgatca tgctcggcat
     5821 cttcttcgtg cacctcgtgt acctgctggc gcgcatcgcg cgcgactggc ggacgttccg
     5881 ctggttcggc ccgacgtcgc tggtgcccac ctggcaggac ctcaaggaca tcggcgcgat
     5941 gttcgcgtgg ttcgtcgggc gccggccgcg gccggccttc gaccgctgga cctactggga
     6001 gaagttcgac tactgggcgg tgttctgggg catggcgatc atcggcggca gcggcatgat
     6061 gctcgcgttc ccgggcgcga ccgcgtcggt gctgcccggc tgggcgctca acgtcgccac
     6121 catcgtgcac ggcgaggaag cgatactcgc cgcggtgttc ctgttcaccg tgcacttctt
     6181 caacaaccac ttccgccccg acaagttccc gctcgacacc gtgatgttca ccggcgccgt
     6241 gccgctggag gaattcaggc gcgagcatgc gctcgagtac cagcgcctgg cggccgcggg
     6301 cgagctcgag cggcacctcg tggacgcgcc ctcgtcggcg atgacgctgg gctcccggat
     6361 tctcggcttc gcgctgatcg cgatcggcct tgtgctgctc gtgctggtga tcatcggctt
     6421 cgcgggccgc accggatgat tgcatggctt atccaccctg gaggcaacat gaaagcgaaa
     6481 ctgttcgttc tggcgatgac aggcgtgctt gcggcgagta gcgccgggct ggtcgcggca
     6541 gccgatgtgc tgaaggcaaa gggctgtttg aactgccacg atgcggagaa gaagaaggtc
     6601 ggtccgtcct ttaacgacat cgcggccaag ttcaagggca agcagggcgc cgcaggcgac
     6661 ctggtggcaa agctgaggga cggcaagggc cacatgaaga tcaaggcttc ggacgccgaa
     6721 ctcgaggccg ccgtgaagca agcgctgggg cagtgacagc cgcggcggcg gcgcctcgcg
     6781 tttgatgtag gtcaagtcgc cccgcgcggc gcgcgataac ttgcgaccga tgattccggc
     6841 ccaggcacgg gcgtgcccaa gcacatcgtt cgattgatcg cgctgctcgc cgcgggcctc
     6901 gcgttggggc tggcggcaaa atggtatttc accgccgatt cgttctacga gtacggccac
     6961 tatcgcgcca aatcggtgcc cgaaatcgcc gcccaggtgc cggcctacca gagcgcggct
     7021 tactgccagg gctgccacgt cgagcgccat gcgcagtggt cggcgaacaa ccacaagtcg
     7081 gtgacctgcg aggtctgcca cggcgcggct cagggccacc cgcagaatgg caagctgccg
     7141 attcccgccg atgcgcgccg gctgtgcacg ctgtgccatg aaaagatggc ggaaaggccg
     7201 cgtacgcagc cacagatcgt ctcggcggag catggcgccg gccagcagtg cgtcgtgtgc
     7261 cacaacccgc atgcgccgaa gctcgcgctc gcggcggcgc tggtcgccgg cgatgccgcg
     7321 gccggcggcc gcaagcacgc ggaaagctgc gccagctgcc acggcgagaa cggcatcagc
     7381 ccgaacgaca cctggccgag cctcgccggc cagaatgccg cctatctggc gcgcatcctc
     7441 ggcgcctaca agtccggcgc gcagagcgac gtgatgatgt cgccggtggc gcaggtgctc
     7501 agcgatgccg atgtgcagaa cctcgccgcc tactacgcgg gcctgagctg ccgggcgccc
     7561 gcgcgcgcag cgggcgccgc cgccgccgcc ggcaagctgc tcgccaagaa ttgcgaggcg
     7621 tgccacggcg agaccggcat caccgccaat cccgcctggc cgcacatcgc cgggcagaag
     7681 tcgacgtacc tcgccaacac gctcaaggcc ttccgcgccg gattacgcaa ggacccgatg
     7741 atggcgggtg tggtcagggg actgagcgat gccgacattg cgaatctggc cgcttactac
     7801 gccgcgcagc gctgcgcacc ggggccgcag tgaaaggggg catgatgaaa cgggattcga
     7861 tcagcgcgat gcgcgcgcgg cgcgggtttc tgcgcggcat cggccgcgcc gctgcggttg
     7921 ccgccgccgc ggctaccgcg ggcaccggcg ccctgcgcca ggccgatgcg aagagcgctc
     7981 ccggcgccgg ccaggccgcg ccgccgatac cggattacga ctggaaccag cacagttggg
     8041 cgttcggcgt cgacgccacg aaatgcatcg gctgcctgcg ttgcgtcgag gcctgcaagc
     8101 acgaaaacga cgtgccgctc accgcacatc acttccgcac ctgggtggag cgctacgtct
     8161 acctggaggg agaggagcat ccgcgcatcg acagccagca ggatccggtc aacattgctg
     8221 ccgcgggttc cgagcgcgaa taccgcttcg ccgaccgcta cgcgggggcg aaagtcgaga
     8281 aggctttctt cgtgccgaag ctgtgcaacc agtgcacgca tcccgcctgc gtgcaggtgt
     8341 gccccgtcgg cgccacctac aagggcgcgg acggcgtggt gctcatcgat cacgactact
     8401 gcatcggctg ccgttactgc gtgcaggcct gcccctacgg cgcgcgctat ttcgacgagc
     8461 gcaagggcgt ttccgacaag tgcacctggt gctaccaccg catcaccaag gggcagcagc
     8521 ctgcctgcgt cgaggtctgc ccggtcggcg cgcgcatctt cggtgatcgc cgcgaccggc
     8581 aaagcccgat cagcctgttc atccgcaata accgcgtgca ggtgctcaag cccgaaaccg
     8641 gcaacgagcc caacgtgtac tacgtcggca tcgacaaggc ggtgctctga tggaacccct
     8701 gatccattac gccccgtaca tcggctgggt gtatccgaac gagacggtca tcgagtggtc
     8761 gatcgccatc gtcgtctatc cctacatcac cggcctggtg gcgggcgcct tcacggtgtc
     8821 gagcctgtac caggtgttcg gcttccagcg cctgaagccc gtggcgcacc tggccctgct
     8881 cacctcgctg tgcttcatga tcttcgtgcc cctgcccctg ctgctgcacc tggggcaccc
     8941 ggaacgcgcg ctcaacaacc tgctcacgcc gcactggacc tcggcgatgg ccatgttcgg
     9001 ctacttcgcg accttctaca tcgtgctgct gatggtcgag acctggttcg cgtttcgccc
     9061 ttacatcgtc gagcaggcgc agaagagaaa gggcgtgctg gggctggcct atcgcctgct
     9121 gacgctgggc tcgtacgacg tgtccgagga agcactgagc cacgaccgca aatggatctt
     9181 ctggctggcg atcgccggca ttcccggcgc gcacggcctg cacggctacg tcggcttcgt
     9241 cttcggctcg ctcaagtcgc gcgaatggtg gtcctcggac ctgatgccgg tggtgttcct
     9301 gttctcggcc atcatctccg gggtggcact gctcatcgtg atctacgtgg cctcgtgctg
     9361 gctgcgccgc atcaggatcg atctcgcctg cctgaagggc ctcgcctaca cggtgtgggg
     9421 attcatcatg gtcgccctgg tgctcgaagg cctggagttc gccaacatcg tgtacaaggg
     9481 ccgcgaaggc gtcgagatga tcattgcgta cgtgaacggc ccgctgctga tcccctattt
     9541 cgttctgcaa ttcgccgtcg gtgcgatcct gccgttgctg tggctttcgt acatgatctg
     9601 gcgcggcacc acgggcaagg cgttcatcac gggcgtcacc ttgtgcgctt gcctggtgct
     9661 actctcggtc ttcatgatgc gctggaacgt ggtcatcggc gggcaggaga tttccaagac
     9721 cggcaggggg ctgctcggct accagctgca gcttctgggc cacgagggcc tgctggtcgc
     9781 gattctgctg ctcctcgcgc cgatcgccac gctcgtcggc ctgacgcgct ttttcccgcc
     9841 ctgggaagac cagcccggat gaggatgctc gtggccagcg tgcttgtcgt gggcgctgcc
     9901 gctacggcat acgcttccgg cgcggcggcg caggccgtgc gcgtcccggc ggggagcgac
     9961 gaccagttgc gcgcggtcta tgccacgccg ctcgaaatcg cggagggcgg gcgcatcgcg
    10021 cagacggcct gcgcccggtg ccacggcgcg aatggcgtca gcaccaccaa gggcgtgccg
    10081 cacctcgcgg gccagcgcgc ggcgtacatc gacctgcagt tgcgcgccta ccgccacagc
    10141 gcccgcgtgc agggcgccat gaccggcgtc gtgcgctatc tgagcgacga cgcgctggtc
    10201 aaggtggccg cctactatgg gagcgtcgag ccgccgcggc ctgcgccggc gcccgccaag
    10261 gccgcgccga aggcggccga tccgctgcag gccggaaagg ccgcggcggc cggctgcggc
    10321 ggctgccacg gcgagtccgg tgtcagcgcg atcgcgggca tgccgagcct ggtcggactc
    10381 gacccgaagt acttcgtcgc tgccgtgaac gcctacaaga gcggcgcgcg caagcacgac
    10441 ctgatgaaat cgctcgtcgc ggcgatgagc gccgagaccg tgagccaggt ggcgctctat
    10501 tacgcgctgc aaaagcccgc caaggcgcag accccggccc ccggtgacgc ggccgcgggc
    10561 aaagccgctg cggcttcctg cgccgcttgc cacggcgaca cgggcgtgag cggcaatccc
    10621 atcacgccca gcatcgccgg ccaggacgcg gagtacttcg tcgttgcgct gcgcgcctac
    10681 aaggacgggg cgcgcaagga cgattcgatg aatggtccag cggcggcgct cgacgaaaaa
    10741 gccatgcgcg acctcgccgc gtattacgcg gcgcagcagc cgcaggcgcc gaacgtgcgc
    10801 cgccccctca ccgccgccga gtggatcgag cgctgcgacc gttgccacgg cgccaatggc
    10861 aacagcaccg acccgctcat gccgcgcctc tcagcgcagc gcgaagattg gctcgaacag
    10921 gtgctcgacg cctaccgcac cggcgtgcgc aagagcacgg cgatggctgc catgtcggcg
    10981 ttgatgagcg aacgagatgt caaggagctt gcggcgcact actcgcgcca gggcgcgcgc
    11041 gcggtcgtgt acctggtgct gccgcccgcg gacaagaaga gcgaaaagtg agcaaggaaa
    11101 cgcgcatgga tgacgcacca ctacaccggt tgtatcgcca tttttccgag ctgccctaca
    11161 gcttgagggt gctgtacacg gcgaccctgc tggtcctcgg caccgcgtac ctgttcgcga
    11221 tgatttacct gttccacacc tactcgggaa aggacggcaa tccgcgctcg gtgtcctacg
    11281 aggacatcgt catcgcctac agcggcagcg gcaagggctc gcgcctggaa gcagcgctgc
    11341 gcgggtccat gtcggccatg ctgccgcgcg acgagctcac atcgctcgtg tcgtgggtgc
    11401 aggagggcgg cgagcgcggc cggtacgaaa aggagatcaa gacggtgctc gacgggcgct
    11461 gcctgacctg ccacgacggc agcaacccgc acctgccgaa cctgaacggc tacgacaacg
    11521 tcctcaaggt caccgaacgc gacaccggca ccgggatctt cacgctggtt cgggtctcgc
    11581 acatccattt tttcgggctc gccttcgtgt tcttcatcct ggggaccatt ttcagccacg
    11641 cctacgtgcg cccggtgtgg ttcaagtgcg ccgtggtcgg gctcccgttc gtttccctga
    11701 cgctggacat agggtcctgg tacttcacca agatctacca cccgttcgcc tgggtggtga
    11761 tgatcggtgg tgccctcatg ggactgtcgt tcgccttcat gtgggtagtg tccatgtacc
    11821 agatgtggtt ctcgcacccg cccaaaatgg tcacgtaccg gcaaggaggc gaaaccaccg
    11881 atgtcggctg atcaactgaa ttcgaggctc gcatgatgaa acgccacacg ttcgtgctcg
    11941 tcattgcgct gtcggcggcg ctcgcaggct gtccgggcaa ggagcaaggg acggcgaagc
    12001 ccgctgtcga gccgctttcg gcgacggacc tggccgcggg aagggccatc gccgagcgcg
    12061 actgcaaggg ctgccacggc ctcgacggcc ggggcgcggc gccggggata ccgcatcttg
    12121 cggcccagcg tgtgggctac ctggtcgcgg cgctggatgc gtacaaggcg ggcaagcgca
    12181 cgcatgccgc gctgcgggag atcgccacgc acctgggcga cgccgaggtg cgcaacgtgg
    12241 cggggttcta cacctccctg ccaccggtac ggtccacagc cgcggtagcg cagagcgcgc
    12301 acgcctcgcc ctacgagctc ggcaagaagg tcgcggcggc ctgcgccaaa tgccacggcg
    12361 aagacggcaa cagcaccacc cccggcattc ccagcctcgc cgggcagcag ccgcattacc
    12421 tcgtcgtggc gatccaggag tacctgttga acgagcgcaa ggcggcgccc atgcacgcgc
    12481 tcctgcccag gctttcgaag ctggacatgg aaaccttggc gctgtatctc gcgtctcaga
    12541 cgcccgcgcc acgcagtgcg cccccgttcg gggaccccgc ggccggcgag cctctgagcg
    12601 ccgtttgcgg cggttgccac ggcgggcacg gcgtaagcac cgacagcgcc acgccgagcc
    12661 tcgcggcgca ggacgcgcgc tacctggtgg acgcgatcaa ggcgtaccgc acgacccgca
    12721 agcgggagaa catgcgcctg tacgtcaccg ggctaagcga caaggacatc gagagcattg
    12781 ctgcgttcta cgccgtgcag aagtcgaggg cggctgaaaa agggcagacg ctggtcaagg
    12841 acctgaccga ccggtgcgaa cgctgccatg ccgccgacgt ggacatcccc ggaatggtcg
    12901 ttcccaagat ccgcggccaa gaccgggact acctggtgat ggcgctgcgc gcctaccgcg
    12961 acgatcgacg cgagagctcg atgatgcaca agatgagcct gccttacagc gactcgatca
    13021 tcgagagcct cgccaccgcc tacgccagcc agccggcgaa ataggcggcg cgcgcggcgc
    13081 gatcagtaca cgcggtgctc gaacttcacc gccactggcg ccggtgcccc cggcgtgcgg
    13141 atgatagcgg taatgcgata gttggcgttc cagcgtagcc ggaagtaagc accgtagctc
    13201 ggcagctggt tgaccatgta gggctcgagc gtcctcgtaa cgcccgacag gccaacttgc
    13261 tcaacgcgca tgaccacctg ggcgtcgccg atcggggttc ttttcctcgc gtcgcgcaac
    13321 gacacgttca agtggtaata gcccgcgccg tccggcaccc cgccatgcag catccgctcc
    13381 gcagaaccct ccggaagcag gcgaagggtt tccgccggca cgatgccgag atccacttcc
    13441 atgccgccgg caatcttgta gcgcggcgcc gccgcgcgcg catccgccgg ggcgtccttg
    13501 ggcgccacgg cacccgggtt gaacatgtag agcaccgcat tgcgcacttc ggcgtcggtc
    13561 aggtcggccc ggtcgccgcg cggcggcatg ccgccatgcc cgtgaatcgc cgcccgcacc
    13621 aggtcgtcga ggccgccctt gagccgcggc gcccaggctg cgatgtcgcc gatcctgggc
    13681 gcgccgccga ggccttcgcg gtggcacttc gagcactgcg cctccacgat ctgcgcgccg
    13741 ctgcgctcgc cggccaggtc cagctcgctc gccggcggca cccagcggcc tcccgagcga
    13801 ttcaccatgt aggtcaccgc acgcgcgatc tcgaggtcgg tgagcgcaag attgccgccg
    13861 tgcgccggca tgccgcgaat gccgtcgagc gcatgtcgcg taagggcggt gaggccctgc
    13921 gcggcgcgat tcttccaggc ttcgctatcg ccgatcttcg gcgcaccgcc gaccccttcg
    13981 ccatgacaac tggcgcaggt cgcctcgacc acctgcttgc cgctgcgctc accgcgctgt
    14041 gcctcggccg cctgcggtgc gatgcacgcc gccgccgcga gcagcgcgct ccatagcagt
    14101 ccggggttca gtcgcatgcc gcggctctgc gcactacttc gtggcgagaa cgtggcgcag
    14161 cgccgcctgc agctcggcat ccgtggcctg gatcttcatg tgtcccttgc cggacttgag
    14221 cttggccaga aggtcggctt ccgcgcccgc gttgcccttg tacttcgccg caacgtcctt
    14281 gtacgccggc ccgaccttct tcttgtccgc gtcgtgacag ttcaggcatc ccttggcctt
    14341 cagcacgtcc gccccggact gtgcctgggc tgccccggct gccagcaaac ccgccgctgc
    14401 cacggctata agtacggtct tcatcgcacc ctcctaagta gactaaaaat ccaatgatag
    14461 cgcactgcgc cagagcggcg cgcgcagcca ggaagcgcgg cagcccgtcg ctggttggcg
    14521 actagatcgg gtcgagcccc cgcccgctca gtaatagccg gtgatgcgac gccttgcgca
    14581 gtgcgtgcgc gacctggtgg ccctcgagca gcccagcgag cttgcgcggg ccgcagtcgt
    14641 ccagcagggt gcggctcacc ttgagcagat gcacgggctc gcggggcttc ttcagcagga
    14701 acgtgtgcgc ttcacggtcc ctgtcccaga cctcggcgag cgcccagccg ggaaagacct
    14761 gctgcaggta cctgcgtacc gcgtcgagac gctcctccgc gatcttcgcc atgccctccc
    14821 cctcgacggc aacacccggt cgcaggaaat gtaacccgaa atttccgcgc gcggtgcacc
    14881 gtcctgctat ctgggaaagt gggtctagtt caggtccagc gcgcactgct tgagcgccgc
    14941 cagcggcacg tactcgagca cgttttcgtg cgtggcctcg agcacgactt gcggcgcggc
    15001 gcccgcgcgc caggcctcga tccagcgcag cacgtgcagg caccagcggt cgcccgcctt
    15061 caggccgcga aagcgcagct ccgggcgcgg cgtgaccagg tcgttgccct tggccacgct
    15121 gaaatccagg aatgcctgcg tcgcccgcac gcaaaccagg tgcgccacgc cgtcggtgcc
    15181 gtgcgtggca cagctgccgt cgcggaaata gccggtgagc ggcgcgtagc tgcaggccct
    15241 cagctcgcca ccaagcacgt ttctctcgct gcgcatgccg cgattgtccc ccagacggcc
    15301 cgacgcccgg ccgagcatgc ggtttgatgc aggtcaaaat tcggcgcgcc ctatgagcga
    15361 cacttattgc gccgcaataa ggagcttcgt gatgctaccg gcgacgacta acaacaacga
    15421 tgcacgtcat gaccgcatcg tggaaagcgt ggtcgccaac atggccatgc tgcgggatgc
    15481 gacgcggagc cagctcgccg agattgccgc gcacgcccgc gcgcgcacgc tgcagcgcgg
    15541 cacggtggcc tgccgcctgg gcgagcccat gcccggcatc ctgatcgtcg ccttcggcct
    15601 gctcaagctg tctttgccgc gctccgaaag cgaggagcgg gtgctgcgct tggtgggcgc
    15661 gaacgagagc ttcggcgagg caccggcgct gcacgaccga cccgcgccct tcgacgccgc
    15721 cacgctgagc gaatcgctgc tggtggtggt gccgcgcgcg ccggtgctgc gcctgatcga
    15781 gcggaactcg gtggtggcgc agagcctggt tgcgcacctc gcggaggact gcctgtcgct
    15841 gctctcggaa ctgcaggcgc tcgcgcagca caacggcgtg cagcgcctcg cctcgtacct
    15901 ggtgtcgctc gccgagcgcg agcagggcgc ggacggctgc caggtgcggc tgccggcgag
    15961 caagacgaca gtggcggcgc ggctcagcat ccagaaagaa acgctgtcgc gcatgctgcg
    16021 cgacctcacg cagcgcggct gcatccgcgt ggagggacgc gcgatcacca ttctcgaccg
    16081 cgaacgactt gccggtctgg ccgcctgagc gctgcgcgca ccgcacgatc cttgactcac
    16141 atcaagaccg gcccgggcac ccgggataaa agtcctccat gttccaggtg cgcatccacg
    16201 gccgcggcgg ccaaggcgtg gtgacggcgg cggagatgct gtcgatcgcc gcatttctcg
    16261 aaggccggca tgcgcaggcc ttccccagtt tcggctcgga gcgcaccggc gcgcccgtgg
    16321 tcgcgttctg ccgcatcgac gaccgcgaga tccgcctgcg cgaaccggtg ctccagcccg
    16381 atgcgctgat cattcaggac ccgacgctgc tgcaccaggt ggacgtgttc ggcggcctcg
    16441 cgcacgacgg ctacgtcctc atcaacacca gccgcacctt cgatgcgctg ggggtgggcg
    16501 aattcgcgcg cagcctgcgc gccgagcgcc agtgcaccgt gccggcaacc gagctcgcgc
    16561 tgaagcactt gggccggccg ctgcccaacg cggcgctgct cggcggcttc gccgccgtgt
    16621 cggacaggat ttccctcgat tcggtcgcgg cggcgatccg ggagaaattc agcggcaagg
    16681 tggccgaggg caacatcgcc gcggcgagcg aggcctatgc cttcgtgcgc ggcgacatgc
    16741 aggagatcag ccatgctcaa gcagactgag ggctcgcacg cggtggccga agcggtcgcc
    16801 ctgtgccgcc cggaggtgat ctgcgcctat ccgatctcgc cgcaaacgca catcgtcgag
    16861 gcactcggca agaaggtgaa gagcggcgcg ctggcgcgct gcgagttcat caacgtcgag
    16921 tcggaattcg ccgccatgtc ggtggcgatc ggcgctgcgg ccgccggcgc gcgcgcctac
    16981 accgccaccg ccagccaggg actgctctac atggccgagg cggtcttcaa cgcctcgggc
    17041 ctcggcctgc cgatcgtgat gaccgtggcc aaccgcgcca tcggcgcgcc gatcaacatc
    17101 tggaacgacc acaccgatgc catgagccag cgcgattgcg gctggatcca gctctttgcg
    17161 gagaccaatc aggaggcgct cgatctgcac atccaggcgt ttcgcctcgg cgaagagctc
    17221 tcgctcccgg tcatggtgtg catggacggc ttcatcctca cgcacgccta cgagcgcgtg
    17281 gacctgccca gccaggagca ggtggacgcc tggctgccgc ccttcgagcc gcgccaggtg
    17341 ctcgatcccg ccgaacccgt gtccatcggc gccatggtgg gcccggaggc gttcatggag
    17401 gtgcgctatc tcgcgcacgc caagcagatg cgcgcgctcg agctgatttc gggctgggcc
    17461 gaagagtttc gcggtgcctt cgggcgcgaa tcgggcggcc tcgtgcatac ctaccgcacg
    17521 gaggatgccg acaccatcgt cgtcgcgctg ggctcggtgc tcggcacgat caaggacacg
    17581 gtggacgaaa tgcgccgcga tggggcaaag atcggcgtgc tcggcgtcac ctgctttcgc
    17641 ccctggccgc atgccgtggt gcgcgcggca ctggcgcagg ccaggcgcgt ggtcgtgatc
    17701 gagaagagcc tcgcggtcgg cctgggcggc atcgtctctt tgaacgtgcg caccgccttc
    17761 ggcggaagcc ccgacgcggt gcacgccgtc attgccgggc tggggggccg ctcgatcacg
    17821 cgcgcctccc tggggcgcgt gttcgccgcc gccatcggcg gcaagctcga gccgctcagc
    17881 ttcctcgacc tcgaccacgc gctggtcgac cgggcgctcg cccgcgagcg cgagcagcgc
    17941 cgctccgggc cgatcgcgga aggcatgctg cgcgacctcg gcgtcatcgc cgcccgcatg
    18001 ggataggaag cacgccatgg ccttccagcc ggtcaagttc taccagacgg gaaccttcac
    18061 ggtcggcaac cgcctgctcg cgcccgagga gcgctcggta caggccaacc tgcgtcgcac
    18121 caattcgctc aattccgggc accgcgcctg ccagggctgc ggcgaggcgc tgggggcgcg
    18181 ctacgccatc gacgccgcaa tggaagcgac gagcaaccgg ctgatcgccg ccaatgcgac
    18241 cggttgcctg gaagtgttct cgacgccgta tccggaaacg tcgtggcaga ttccctggat
    18301 ccactcgctc ttcggcaaca ccgcagcggt cgcgaccggc atcgccgcgg cgatgagagt
    18361 aaaagggcgt gacgacgtgc gcgtcgtggc gcagggcggc gacggcggca ccaccgacat
    18421 cggcttcggc tgcctctcgg ggatgttcga gcgcaacgac gatgtgctct acatctgcta
    18481 cgacaacgag gcctacatga acaccggggt gcagcgctcc tcggcgacgc cgcctgccgc
    18541 gcgcacggcg accaccatgg cggtgggcga agcgcccggc aacgtgttcg gcccgggcaa
    18601 gaacctgccg gcgatcgcca tggcgcacgg cattccctat gtggcgacgg cgaccgtggc
    18661 cgagctgcac gatctggagg ccaaggtgcg ccgcgccatg ggatttcgcg gcgcgcgcta
    18721 cctgcacctg ttcgtcccct gcccgctcgg ctggggccac gccgccgagg acacgattcg
    18781 cattgcgcgg ctggcaaagg aaacggggat gtttccggtg ttcgaggcgc tgtacggcga
    18841 agtcgcgtcg gtgtcgaaga tccgcaggaa gctcccggtc gaggagtacc tgaggccgca
    18901 aaagcgcttc gcgcacctgt tcggcgccac gccgcgggcc gaggacatcg cgcgcatcca
    18961 gggcatcgcc gaccgcaaca tccgccgtta cgggctcgtg gatgcgtaag cccttcgcca
    19021 tcacgctcga tcccggatcg agcctcgcca accacaccgg cacctggcgg acgctgcgcc
    19081 ccgagtacgt cgatcgcctg ccgccctgca acagcgcctg ccccgccggg gagaacatcc
    19141 agggctggct ctaccacgcc gaatcgggcg actacgaggc ggcgtggcgc ctgctgacgc
    19201 aggacaaccc gctgcccgcg gtcatgggac gcgtctgcta ccacccctgc gagacgtcct
    19261 gcaaccgggc gcagctcgac tccgcggtcg gcatcaactc ggtcgagcgc ttcctcggcg
    19321 acgaggcact gcgccagggc tggaagttcg acgcgcccgc ggcgccctcg ggaaagcgcg
    19381 tgctggtggt gggcgccggc ccctcggggc tctccgcggc gtaccacctc gcgcggctcg
    19441 gccacgcggt gcagatccac gaggccggcc cggtcgcggg cggcatgatg cgctttggca
    19501 tccccaagta ccgcctgccg cgcgacgtgc tcgaggcgga gatccgccgc ctgctcgagc
    19561 tgggggtgac gctcaagctg aacgccaagg tggcgaacat cctcgagacg atgcaaggcg
    19621 gtgcgttcga cgccgccttt ctcgccgtcg gcgcgcacat cgcaaagcgc gcctacatcc
    19681 cggccggcag cgcggccaag gtgctcgacg cgctgtcggt gctgcgctcg atggaaggcg
    19741 aagaaaagcc gctgctcggc cggcgcgtgg tggtctacgg cggcggcaac accgccctcg
    19801 acgtggcgcg caccgccaag cgcctgggcg ccgaagacgc ggtcatcgtc taccggcgca
    19861 cgcgcgagaa gatgccggcg cacgactccg aggtcgagga agcgctgcag gaaggcgtgc
    19921 tcatcaagtg gctctccacc atcaagcagg ccgccgaatc ctcgatcacg gtcgagaaga
    19981 tggtgctcga cgccaagggc aacccgcagc ccaccggcga gctggagacg ctcgccgccg
    20041 attcggtggt gctcgcgctc ggccaggacg tggacctgtc gctgctcgat ggcgtgccgg
    20101 gcctggaaat gcgcgacggc accgtgcagg tcggcgccaa catgatgacc ggccacgacg
    20161 gcatcttcgc cggcggcgac atggtgcccg ccgagcgcac ggtgacggtg gccgtcgggc
    20221 acggcaagaa ggccgcgcgc cacatcgacg cctggctgcg cggcgccgtg tacgcgccgc
    20281 cgcccaagca cgagccggca acattcgagc aggtcaatac ctggtactac tcggatgcgc
    20341 ccaagaccgt gcgcccggtg ctcgacggcg tgcgccgcca gtccaccttc gacgaggtgg
    20401 tgcgcgggct cgacgagtcc aacgccctgt atgaagcgcg gcggtgcctg tcgtgcggca
    20461 actgcttcga gtgcgacaac tgctacggcg tgtgcccgga caacgccgtg atcaagctcg
    20521 gaccgggcaa gcgcttccag ttcaacttcg actactgcaa gggctgcggc atctgcgtcg
    20581 cggaatgccc ctgcggcgcg atcaagatgg tgccggaaga gatatgaacg cccggctgcc
    20641 cgccgggctg cgcggcatgg cggtgctgcg cgacccgttg ctcaacaagg gcaccgcgtt
    20701 cacgcagaag gagcgcgccc gtctcggcct gcgcgggctc ctgccgccgc acgtgcatac
    20761 ccaggaagag cagatgcagc gcagccttgc gcacctgcgc ggcctgccca gtccgctgga
    20821 aaagttcgtc aacctgaacg cgctgcacga ccgcaacgag gcgcttttct tccgcatcgt
    20881 cgcggagcat cccgacgaga tcatgccgct catctacacg ccgaccgtcg gcctcgcctg
    20941 ccagaagttc ggcaacatct tccagcggcc gcgcggcctg ttcatcggcg ccaacgaccg
    21001 cggcaagatc gcccaggtgc tgcgcaactg gccgcagcgc gacgttggca tcatcgtcgt
    21061 cagcgacggc gagcgcatcc tcggcctcgg cgacctgggt gcgaacggca tgggcatccc
    21121 ggtcggcaag ctctcgctgt ataccggctg cgccggggtg catccgcggc gctgcctgcc
    21181 cgtgatcctc gatgtcggca ccgacaacga ggccctgcgc aacgatccgt tctacgtcgg
    21241 gctgcgccag acgcgcctgc gcggcgcgcg ctacgacgcg ctggtggagg agttcgtgac
    21301 cgcggcgcag aaggtgttcc cgggcgtgct gatccagttc gaggatttcg ccaacaccaa
    21361 cgccttccgc ctgctgcagc gctaccgcga gcgcgtctgc accttcaacg acgacatcca
    21421 gggcaccgcc gcggtagtgc tcgccggcct gtactcggcg ctgcgcgtca cgggcgccgc
    21481 gctgcgcgag cagcgcatcc tgtgcctcgg cgccggcgag gcggcaagcg gcatctgcga
    21541 cctggtggtg gccgcgctcg gcgacgaggg cctcgccgcg gacgcggcgc ggcggcgctg
    21601 ctggctgttc gattcgcgcg gactggtggt ggcgggccgc accgatctcg ccgcgcacaa
    21661 gcggccctat gcccacgagg gccagcgcgt cggcgatttc gccgccgcgg tcgaggcgct
    21721 caagcccacc gccatcatcg gcgtggccgc ggtcggcggc accttcaccg agaaggtggt
    21781 cgcggcgatg gcgcgcatca acccgcggcc gatcgtgttc gcgctctcga acccgacctc
    21841 gcaatcggaa tgcaccgccg agcaggccta ccactggtcg ggcggccggg cgctctttgc
    21901 ctgcggcagc ccgttcgacc cggtgacgct cgacggacgc gtattcgtgc cgcgccaggg
    21961 caacaattcc tacatcttcc cgggcgtcgg cctgggagtg atctcgagcc gcgcgcggcg
    22021 cgtcaccgac gagatgtttc tcgccgccgc gcgcacgctc gcgcggcaga cgacgcaggc
    22081 cgacctcgac cagggcagcc tgtatccggc gctcgcacgc atccgcgacg tttccgcgcg
    22141 gatcgcggcg gcggtagcgc gggtcgccta tgcgcgccac ctcacctcgc agcgccggcc
    22201 ggccaatctg ctcgctcaca tccgcgcgca gatgtacgac ccgaagtatc cgcgctacgc
    22261 ctgagacgcc tcgatggcgc gcttcacggc gcgcgcatcc ggcacgatca cttcgacgcg
    22321 ctggggcagc tgctccaggc cctcgaacct cgccgggcgc ggcggctcac gccccaacgc
    22381 ctgcgcgatg gtggcggcga acttcacggg cagcgcggtt tccaggcaga tgagcggcac
    22441 gcccggggcg cgctgctcga gcccgacctt cacgccgtcc gcggtgtgcg gatcgacgac
    22501 cacgccgtat ttccgctgca actcgcggat ggtcgccagc cggtcttcgt gcgtgctctt
    22561 gcccgagacg aacccgcttg cccccggcag ctcgaattcg ccgtcgcggg caagctgcgc
    22621 ccagagcgcg acgatgcgcc cgggatcgcg tcccaccagc tcgaaggcgt agcgctcgaa
    22681 gttggaggct ctggagatgt ccatcgaggg gctcgaggtc gcgcgcaccg cgcggcgcac
    22741 gcggtagcgc ccggtgcgga agaattcgtc cagcacgtcg ttctcgttgg tggcgaggat
    22801 caggcgcgac acgggcaggc ccatgcggcg cgccacgtag cccgcgtaga tgttgccgaa
    22861 attgcccgag ggcaccgcga aatccacgcg ctgcgcgtcc gagcgcgtgg cgcgaaagta
    22921 gccccagaag tagtacacca cctgcgccgc gatgcgcgcc cagttgatcg agttcacggc
    22981 gccaatgcgg tgccgcgcct tgaacgccgc gtcggcgttc acctgcttca ccacgtcctg
    23041 gcagtcgtcg aacgtcccct cgacggcgag gttgtggata ttcgcatcct gcagcgcgta
    23101 catctgcgcg gcctggaaag cgctcatgcg cccgtgcggg gacagcatga acacctgcac
    23161 gccgcgccgg ccgcgcagcg catgctcggc cgccgagccg gtgtcgcccg aggtcgcgcc
    23221 gagcacgttc agcgtctgtc ggcggcgcac cagcacatcc tcgaagagga ccccgagcag
    23281 ctgcagcgcc atgtccttga aggcgagcgt gggcccgttc gacagcccga gcaggtgcag
    23341 gcccggctcc agtgtcttca gcggcgcgat gtcatcgctg ccgaacacgt cccgcgtgta
    23401 agtgcgcgcg acgatcgtct cgagcgccgg cacgtcgtcc atgtacggcg cgagcaccgc
    23461 cgccgcgagc tgcggatagc cgagcccgcg ccaggcggcg agatcggccg gcgggaagga
    23521 ctcgggcacg tacagcccgc cgtccggcgc gaggccctcc agcaggatgt cggagaagcg
    23581 ctgcggcgcg gcgccgccgc gagtgctgac gtagcgcact acagcaggga ttcgaggcgg
    23641 atgcgggtga ccttgccgag caccgtgggc aggcgctcga tgcgcgcgag cgctgcatcc
    23701 acgttctttt ccacggcgag gtgggtcagc atgacgatgt cggcccgctt ctcgcccgcc
    23761 ggcggctcct tctgcaccag ggcctcgatc gagatcttcg agtcggcgag gatgcgcgtg
    23821 acgcgcgcca gcacgccggg cttgtcctgc acgcgcaggc gcaggtagta agaggtctcg
    23881 acctcgccca tcggcaggat cggcgcggcc gagagctggt cgggctggaa cgcaaggtgc
    23941 ggcacgcggt ttcccggatc ggcggtgatg aggcgcgtca cgtccaccag gtcggcgacc
    24001 accgcgctcg ccgtgggctg gctgccggcg cccgcgccgt agaacatcgt cggcccgacc
    24061 gcgtcgccct ttaccaggat ggcgttcatc acgccctcga cgttggcgat caggcgccgc
    24121 gtcggcacca gcgtcgggtg cacccgcagc tcgatgccct tcggcgcgcg ccgcgtgatg
    24181 ccgagcagct tgatgcggta gccgagctcc tcggcgtagc cgatgtcggc ctgcgtcagg
    24241 cgcgacaccc cctccgtgta ggccttcgcg aactgcatcg ggatgccgaa ggcgagcgcc
    24301 gacaggatcg tcagcttgtg cgccgcgtcc acgccttcga tgtcgaaggt gggatcggcc
    24361 tcggcgtagc cgcgcgcctg cgcgtcggcc agcgccacgc ggaaggcgag gcccttgtcg
    24421 cgcatctccg agaggatgaa attggacgtg ccgttgatga tgccggcgat ccattcgatg
    24481 cggttggcgg cgaggccctc gcgcagcgcc ttgatgatcg ggatgccgcc gcccaccgag
    24541 gcctcgaagg cgaccatcac gccttttttc tgcgcagcgg cgaagatgcg gttgccgtgc
    24601 ttggcgagca gcgccttgtt cgcggtaacc acgtgcttgc cgtgcgcgat cgcctcgagc
    24661 acgaggtcgc gggcgatgcc ggtgccgccg atcagctcga cgacgatgtc gatgcgcggg
    24721 tcgcgcacca cttcccaggc gttgccggtg acctcggcct tgccggcgac cagcttgcgc
    24781 gccttgttcc ggtccttgtc ggcgacgcgc gcgatgcgga tggcgcggcc ggcgcggcgc
    24841 cggatctcgc cggcgttgcg gttcagcacg tcccaggtgc cgcccccgac cgtgccgatg
    24901 ccgagcaggc ctacctggat gggcctcatg ccgcgccgcg cagcagaccg tctttgcgga
    24961 acatctcctt cacgccgcgc agcgcctggc gcaggcggtt ctcgttctcg atcagcgcga
    25021 tcctgacgtg gtcgtcgccg tagtcgccaa agccgatgcc cggcgccacc gcgatcttcg
    25081 cctcgcccag caggcgcttg gtgaactcga tcgagcccat ggcggcgtag gcctccggga
    25141 tcttcgccca gacgtacatc gaagccttcg ggttctccac ccgccagccg atgtcgtgca
    25201 gccccttcac catgacgtcg cggcgccgct ggtaggtcag gcggatctcc tcgacgcagt
    25261 cctgcggccc ttcgagcgcc acgatcgagg ccacctgcag cggcgtgaac gtgccgtagt
    25321 cgtggtagct cttgatgcgc gccagcgcgt gcaccaggtc ctggttgccg accatgaagc
    25381 cgacgcgcca gcccgccatg ttgtagctct tcgacatggt gaagaactcc accgccacgt
    25441 cgcgcgcgcc cgccacctgc atgatcgagg gcgccctcca gccgtcgaac acgatgtcgg
    25501 cgtaggcgag gtcgtgcacc accatcatgt cgtgctcgcg ggcgagcgcc accacgcgct
    25561 cgaagaaatc gagctcgacg cacttcgccg tcggattgga ggggaagccg aggatcagca
    25621 tcttcggctt cgggatcagc tcgcggatcg cgcgctcgac ctcggcgatg aagtccacgc
    25681 ccggggtcat gcgcacggag cggatgtcgg cgccggcaat gatcgcgccg tagatgtgga
    25741 tcgggtacga gggattgggc accagcaccg tgtcgccgcg atcgagcgtg gcgagcatca
    25801 ggtgcgcaag gccctccttc gagccgatgg tgacgatcgc ctcgctgtcg ggatcgaact
    25861 cgacgccgta gcgccgccgg taccagtcgc acaccgcctt cctcaggcgc ggaatgccct
    25921 tggagaccga gtagccgtgc gtgtcctggc gctgcgcggc ctcgaccagc ttgtcgacga
    25981 tgtgcttcgg cgtcggcccg tcgggattgc ccatcgacag gtcgatcacg tcctcgccgc
    26041 gccggcgcgc cgccatcttc aactcgttgg tgatgttgaa gacgtacggc ggcagccgct
    26101 tgatccttga aaactcacgc attgtagaat tctagcagtg aaacttcagg cctccgctcc
    26161 cgcacgcgtc aacgccttca ccggctacgg cgacggctac gtgctgctga acggggcgcg
    26221 ccgcgaggcg agcgtgatcg tgctccccga gcgcgtcgtc gagtggccgg tggcgtcctt
    26281 cgccgcgctc gccgaggcgg atttcgaacg catcgcggcg ctcaagcccg aagtggtgct
    26341 gctcggcacc ggcgcgcggc tgcgctttcc gcacccgggg ctcgccgcgc cgctcgcgcg
    26401 cgcgggcatc ggcctggaag tgatggacct gaaggccgcc tgccgcacct acaacatcct
    26461 cgccgccgag gagcgcgtgg tggcggcggc gctggttttc gcgtgacgcc ggtcggtctt
    26521 gcgctggtgg ccgttgcggt gctcctcggc actgcggggc agatcctgct caaggccggc
    26581 accaatgcca tcgggcattt cgagttcacg ctctccaatg tgctgccggt gggattacgc
    26641 gtggcgctcg agccgcgcat catgggcggc gttacctgct acgcggtcgg catggtggtt
    26701 tggatcatgg cgctctcgcg caccgacgtg agcgttgcct acccgatgct ctcggtgggc
    26761 ttcgcgctga acgcgctgct cgcctggtgg ctgctcggcg aaggcgtgaa cgcgcagcgt
    26821 ctcgccggca tcgcggtgat catgctcggg gtctggctgg tggcgcgctc gtagatggcc
    26881 gacgcgctcg gctggcgccg caagctcgcg gtgctcgtgc cctccaccaa caccgtggtg
    26941 cagccggagt tcgacgccat gcgcccggac ggcgtcacca accacatcag ccgcatcc
//
LOCUS       DBNHKE010000053        29781 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_695881, whole genome shotgun sequence.
ACCESSION   DBNHKE010000053 DBNHKE010000000
VERSION     DBNHKE010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 29781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29781
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_695881"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..587
                     /locus_tag="AC16O9_03270"
     CDS             168..587
                     /locus_tag="AC16O9_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6515670.1"
                     /translation="MLAKKKSPAPRHRLLIVDDEESMRLLLARQASKGLKAEVQLAGT
                     CEQALRLARNYAYDAILLDLMMPGIGGFEVLKAIRASSANMATPILIISALEDLATND
                     RCLAAGASGFLVKPVEQRALEIAVKSLLEARGKAMNT"
     gene            596..982
                     /locus_tag="AC16O9_03275"
     CDS             596..982
                     /locus_tag="AC16O9_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6515671.1"
                     /translation="MAQIKIGNVEALGKPLGQYSHLARVKNATETLYIAGMLAPGDDF
                     DTQCSGVFRQVEAALKSAGAGWANVAQFTTYLVHSQDIPKFMAWRLREFPKLFPDGKY
                     PPNTLLVVDRLVQEPFLIEVQAIAAL"
     gene            complement(986..2500)
                     /locus_tag="AC16O9_03280"
     CDS             complement(986..2500)
                     /locus_tag="AC16O9_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR6515672.1"
                     /translation="MEVLDNLILGAQVAFSWQNVLYCLLGVLLGTLIGVLPGIGPVPT
                     IAMLLPITFNLDPTAALIMLAGIYYGAQYGGSTTAILVNLPGEASSVVTVLDGYQMAR
                     QGRAGPALAISAIGSFVAGCVATLIIATAAPPLAEVALKFGPAEYFSLMVLGLVAATV
                     LAHGSLVKAIAMVVLGLLLGLVGTDVNSGVLRFTFGIPELADGVGFVIVAMGMFGVTE
                     IVANLEQRGKREVFTGKLTHLFPTREDLKRSWAPILRGTGLGSCLGILPGGGAMLSSF
                     ASYTVEKKISKHPEQFGKGAIEGVAGPESANNAGAQTSFIPMLTLGIPGNAVMALMIG
                     ALMIQGIAPGPQVMTDKPQLFWGLIVSMWLGNAMLVVLNLPLIGMWIKLLRVPYRFLF
                     PSILIFMNVGVFSLSNNPWDCLIMSAFGVFGYVCVKLECEPAPMILGFILGPLMEENL
                     RRAMLLSRGDPTVFFNPMAKPISFSFLLAAVALLVIIALPNIRKKREEVFVEEP"
     gene            complement(2505..2975)
                     /locus_tag="AC16O9_03285"
     CDS             complement(2505..2975)
                     /locus_tag="AC16O9_03285"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR6515673.1"
                     /translation="MRIRSPRDFWAGMMFVGFGLFFVAWSFAHYQMGSAVRMGPGYFP
                     AMLGGLLILLGAIVLLQSLALDGPKVAAFHFKPLVLVTLSVLAYGYLMQPGGLAIATA
                     AAVFIGALAGHEFKWKEVTVVALVLVIFSYLVFVKGLTLPFPMCPSFIENCPIR"
     gene            3017..3631
                     /locus_tag="AC16O9_03290"
     CDS             3017..3631
                     /locus_tag="AC16O9_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR6515674.1"
                     /translation="MQRVPVFRAFLVSVALLAAACGDEPKLAPLAPDALVLAFGDSLT
                     YGTGAREAESYPAQLEALIGRRVVRAGVPGEATAQALKRLPGELDEYQPQLLLLCIGG
                     NDFLRNLGKAQAAANVQTMVAVARQRGIDVLLIGTPEKGLTVTPPAFYAEIAAQHRIP
                     YEGRVIGEILRDSELKSDTIHPNARGYRLIAERVAALLRKSGAL"
     gene            complement(3609..4346)
                     /locus_tag="AC16O9_03295"
     CDS             complement(3609..4346)
                     /locus_tag="AC16O9_03295"
                     /inference="COORDINATES: protein motif:HMM:NF022694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HIR6515675.1"
                     /translation="MKRRRFLAAAALGLLPGTLRAAEPVYAMRFSALAAGARLPPEYR
                     AYAFAGQDRRTQYDLVEDAGRTVLRARAEASTAGIIRDLKVDPATHPVLAWRWKATRL
                     PEKSDLRSKSGDDYAARLYVTFDLRLAALPFGERFGVTLARVIYGDDVPSAALCYVWA
                     SNATVGTVVPNAYTGRVQMIVVESGAAQLGRWSDYERDVAADYRRAFGAEPPAVSGVV
                     VSTDTDNTGERAEAYYGDIEFRARPTS"
     gene            complement(4343..4882)
                     /locus_tag="AC16O9_03300"
     CDS             complement(4343..4882)
                     /locus_tag="AC16O9_03300"
                     /inference="COORDINATES: protein motif:HMM:NF021365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6515676.1"
                     /translation="MLVFAREARPGRVKTRLIPLLGAHGAARLHARLVRRALATARAA
                     GLGEVTLWSTPRQRGPDLGARMHHAFARNLRRAPRVIVMGADAPALRPAHLRRAARWL
                     AGGADAVFAPAEDGGYALVALRRLTPRLFEGIEWGGPQVMAQTRARLAALGWRWRELP
                     EVWDVDRPADVARLRELRR"
     gene            complement(4884..5555)
                     /locus_tag="AC16O9_03305"
     CDS             complement(4884..5555)
                     /locus_tag="AC16O9_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6515677.1"
                     /translation="MVAERLTVVVPTLNEAAGIEACLAALAPLRARGNEVIVVDGGST
                     DATARLAVPLADRVLAAPRARAAQMNAGAAAAAGDALLFLHADTRLPPDADRLVGAAL
                     ADHAWGRFDVAIDSDDPRLAVIAFFINRRSRLSGIATGDQAIFVRRDAFPGYPPIALM
                     EDVAFSRAMKRVSPPACLADRVVTSARRWERRGVARTVLLMWRLRYDYWRGADPDELA
                     RRYSH"
     gene            complement(5516..6283)
                     /locus_tag="AC16O9_03310"
     CDS             complement(5516..6283)
                     /locus_tag="AC16O9_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR6515678.1"
                     /translation="MDARLIVLTAAFAALPAQAAFDHSHAAWDALVKRHVKVLPGQHA
                     SQMDYAGMAGDRAALKAYLDGLSKVPKAEFDAWPKNERKAYLINAYNAFTVEKILTRW
                     PDIRSIWDFGKIFGNPFKDDFFRLLGKPMTLDGIEHGTLRRPGAYDDPRVHYAVNCAS
                     IGCPMLREEAYVAGRLEAQLDEQAVRFLSDRTRNRYRDGRLEVSKIFDWFEEDFVPYE
                     KYFAQYAKVLGYPGGPVPVDFLDYDWSLNGLRSSSPR"
     gene            complement(6268..8409)
                     /locus_tag="AC16O9_03315"
     CDS             complement(6268..8409)
                     /locus_tag="AC16O9_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6515679.1"
                     /translation="MNRTRLLVLTIVVVLVGAFFALGGERYLTLEQLRAQQDAAQAYF
                     LSHPWQTALGYFLVYVTITGLSLPGAAVMTLFGGAVFGLLWGTVIVSFASSAGATLAF
                     LVSRFLLRDWVQGRFGDKLKPINDGVAREGAFYLFALRLVPAFPFFVINLVMGLTPIR
                     TWTFYWVSQLGMLAGTLVYVYAGTQLAEFRLSAELVVAFTLLGLFPLAAKKALDAVKA
                     RRVYARWADQKPKQFDRNLVVIGAGSAGLVASLIAAAVKSKVTLVEKHKMGGDCLNTG
                     CVPSKALIKTARVLSTIRRAKEYGLTNAGAEFDFADVMERVQRVIRTIEPHDSAERFE
                     GLGVECVQGEAKIASPWIVEIQKPDGSTQVLTTRSIVIAAGARPFVPPIPGIEKTGYY
                     TSDTVWELRELPRRLVVLGGGPIGSELTQAFQRLGAQVTQVEMLPRLLIREDPEIAAM
                     VESRFRAEGVDVRLGHKAKEFRVEDGRKVLICEHQGKDLAIEFDALLVAVGRVANTAG
                     YGLEELGIPVTKARTVETNEYLQTLYPNIYAAGDVAGPFQFTHTGSHQAWYATVNALF
                     GSLKKFRADYSVIPWATFTDPEVARVGLNETEAKARNIPHEVTVYGIDDLDRAIADEE
                     AHGVVKVLTVPGKDRILGVTIAGEHAGDLIAEYVAAMKHGIGLNKILGTIHIYPTLAE
                     ANKYAAGVWKRAHAPERLLTYVERFHAWMRG"
     gene            complement(8440..8982)
                     /locus_tag="AC16O9_03320"
     CDS             complement(8440..8982)
                     /locus_tag="AC16O9_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6515680.1"
                     /translation="MTGRPQQLAHLEYLGDAARFAHQLHALIPQCPLLENFTPDEVRM
                     LSHFMDVYRAPAGAEIIREGDGGDFMMMVIEGRIEVRKLDRWNTPQVIATVEPGRTLG
                     EMSMIDGAARFATCVAADGALVAVLDRESLARIIVEQPMLGAKILMELVLMLSQRLRA
                     TSDRLMQLLDEQGQRAQGLL"
     gene            complement(8998..9867)
                     /locus_tag="AC16O9_03325"
     CDS             complement(8998..9867)
                     /locus_tag="AC16O9_03325"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIR6515681.1"
                     /translation="MSARDLLLLLRPHHWVKNGFVLTGLLFGHGWGDPHYVAAALVAA
                     LAFCLASSAVYAFNDAQDAVHDREHPEKRNRPVARGAITPRAAQGIALLLAVAALGIA
                     FAAGVRVAAIVAAYIVLTAAYSLGLKHVAVLDVFIIAAGFMLRILAGTWGIGIEPSRW
                     LLACGFLLTLFLGFAKRRAELDRLADDAGQHRAVLEDYSLAFLDRAVFACALGMVVTY
                     ALYTVAETTVALHGTERLIWSLPWVLLGTFRYLFRLHYRGGGGDPAQELLRDPLLAAA
                     ALGWVATVSFLIG"
     gene            complement(9864..11345)
                     /locus_tag="AC16O9_03330"
     CDS             complement(9864..11345)
                     /locus_tag="AC16O9_03330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6515682.1"
                     /translation="MVSLTLRQWFWLALAATLAFRFWLGSALPITSDEAYFALWGRYP
                     DLGYYDHPPMVGWLLAPLVAVSHAEWFVRLPSILLPATLALLVRHALSAWFARDADTA
                     DTAALAVLLVPMNVWNVFITTDTPLALFAVASLLAFARQRFLLAGVLLGFAFLSKYFA
                     VLLGLGYLAWAIAARRPGAFGLAFLGALPAGLLNLYWNWQACWCNVMFNAINRHDDAG
                     LSWTTPALYAASLAYLAAPLLWFAWKERARLRAALPRVEVGVTVAAWLVPLALFALLS
                     PVKRIGLHWLLAFVPALVLSLALGLERRALVVTLRWFGALAVLHAALLIGLLVPPLET
                     WRDTPLYQRLVLLFEPREVLEPFGPLMARYRLVTEGYSVAAQLAFHTRRRVPVFGGGS
                     SHARHDDILTDWRRLDGRDLLVFRREPPELDAYRPYFREVEAREASARGVTHYAVLGR
                     GFDYAAYRERVLAVVSKRWYRIPTWLPVGGCYYFERYGFERPR"
     gene            complement(11370..12485)
                     /locus_tag="AC16O9_03335"
     CDS             complement(11370..12485)
                     /locus_tag="AC16O9_03335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6515683.1"
                     /translation="MTTPIDPFLGSVPADKTSLTDDERIENIVPLPPPEHLIRFFPIQ
                     GTPVEALISDTRRRVREILHGKSDRLLVVMGPCSIHDPKAALEYAGRLAAERRLHEGE
                     LELLMRVYFEKPRTTVGWKGLINDPYLDGSFRINEGLRIARDLLVRINQAGVPAGCEF
                     LDVISPQYLGDLVSWGAIGARTTESQVHRELASGLSAPVGFKNGTDGNVKIAVDAILA
                     AGQKHHFLSVHKTGQVAIVQTRGNDDCHIILRGGKSPNYDAASVAAACAELAKAKIPE
                     RLMVDCSHANAAKQYKRQLDVTRELAAQIAGGERRIVGTMVESHLVEGRQELAPGKAL
                     QYGQSVTDPCLGWKDSIEVLESLAGAVKERRKHAKRR"
     gene            complement(12660..13091)
                     /locus_tag="AC16O9_03340"
     CDS             complement(12660..13091)
                     /locus_tag="AC16O9_03340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6515684.1"
                     /translation="MSSFRPELTPEVFNGRSRGHLPGLLGLQILTMSAQAVESRLEVR
                     KDHMAPNGFLHAATVIALADTSCGYGCVAQLPQGASGFTTLELKANFLGTARDGAIVC
                     RARPVHLGRSTQVWDAEVSVEGAQRRIATFRCTQMVLWPAQ"
     gene            13236..14189
                     /locus_tag="AC16O9_03345"
     CDS             13236..14189
                     /locus_tag="AC16O9_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6515685.1"
                     /translation="MAIPIAGFKDVYDVPAVEKARHGLPASANEALRALYDKMLRVGG
                     QRFTVKPSAMPPMNELFEDLPNFGRVLEDIRKQLALCMDSEDAVELPPMLLLGAPGIG
                     KTHFARRIAQLLGTGCGFVPMSSLTAGWILGGASSQWKNAKPGKVFETFLNGEYANPV
                     MVVDELDKTSADGQYDPLGALYGLLEVDTATRFVDEFAEIPIDASGAVWFATANDASR
                     IPEPILNRMHVYEIDPPDAEGAARIALAIYREIRGAHDWGKRFPEAPAAAVVDRLAAL
                     SPREMRRAVLAAFGNAKVAGRAELSPDDVQDARGPRRRIGF"
     gene            complement(14202..14678)
                     /locus_tag="AC16O9_03350"
     CDS             complement(14202..14678)
                     /locus_tag="AC16O9_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HIR6515686.1"
                     /translation="MSPIDALAASALARAMQESRWLGPAALAAHLGGVAVLLGTIAVV
                     DLRLLGLWRGLSVRRLAGRLLPWTAASFLVIVPAGLLMFVAHAGELIDSGMFALKMGF
                     ILAAGVNAGIFHAGAYRSAAAWDAGRMPPPAARLAGGLSLALWLGVLACGFLLPER"
     gene            complement(14690..15157)
                     /locus_tag="AC16O9_03355"
                     /pseudo
     CDS             complement(14690..15157)
                     /locus_tag="AC16O9_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            complement(15141..17756)
                     /gene="alaS"
                     /locus_tag="AC16O9_03360"
     CDS             complement(15141..17756)
                     /gene="alaS"
                     /locus_tag="AC16O9_03360"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR6515687.1"
                     /translation="MNSNEIRAGFLEYFESKGHAVVASSSLVPAGDPTLLFVNSGMVQ
                     FKDVFLGKDKRPYSRATTSQRCVRAGGTHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     SDAIQFAWELLTKVYRLPPEKLWTTVYETDDEAFELWTKTIGVPKERCIRIGDKPGGQ
                     KYQSDNFWQMDDTGPCGPCSEIFYDHGPGIAGGPPGSPEADGDRYIEIWNLVFMQFNR
                     DDQGVMHPLPKPSVDTGMGLERIAAVLQGKHSNYEIDLFQDLINAAARETKQKPLKNP
                     SLNVIADHIRACAFLIVDGVIPGNEGRGYVLRRIVRRAIRHGYKLGQKQPFFHRIVED
                     LDKAMGAAYPELSRDKARVIAVLKQEEERFAETLENGMGVLESALASGEQKLLDGETA
                     FRLYDTFGFPVDLTADICRERGVMLDMAGFEAAMAAQRERARAASKFSAAAGLEYAGG
                     KTEFRGYDTLAVQARVVALYREGAQVEALRSGEAGTVVLDQTPFYAESGGQVGDRGAL
                     VAATGTFEVGDTQKIQAEVFGHQGTLKTGTLKAGDTVEAKVDTQLRARTMRNHSATHL
                     MHKALREVLGSHVQQKGSLVDEDKTRFDFAHDKPMSEAEIRRIEAQVNAEILRNVSTK
                     AQIMPIEDAKRSGAVMLFGEKYGDEVRVLDIGSSREFCGGTHVARTGDIGLFKIVEQS
                     AVAAGVRRVEAKTGEGALAWVQAQEAKLRQAAAALRAPVDDVESKIAQVLDNVKSLER
                     ELAKLKSRLASGQGTDLAGQAVDVKGAKVVAATLDGADAKTLREAVDRLKGELKSAAI
                     VLGAVADGKVALIAGVTADLTARVKAGELVNYVAQQVGGKGGGRPDMAQAGGTESAKL
                     PAALQSVRAWIEQRL"
     gene            complement(17810..18148)
                     /locus_tag="AC16O9_03365"
     CDS             complement(17810..18148)
                     /locus_tag="AC16O9_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515688.1"
                     /translation="MDQAQALAIVRSLANGVDPESGEVFPAEGAYQRPQTVRALYTAM
                     EALERAAQFERRKSQLPAKTGEPWSEDEDRRLLAAFDAGHALGELAAAHQRTQTGIRA
                     RLVKYGRLAA"
     gene            complement(18180..18662)
                     /gene="recX"
                     /locus_tag="AC16O9_03370"
     CDS             complement(18180..18662)
                     /gene="recX"
                     /locus_tag="AC16O9_03370"
                     /inference="COORDINATES: protein motif:HMM:NF001055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIR6515689.1"
                     /translation="MRRRESAAEAPDTAQELKARALRHLARREHSRAELARKLAPHAE
                     SPEALGQLLDALAAKKQLSDERYAEARARQLARKYGAARIRQDLKAKGVDEGIVERVS
                     AEDEAVRATAILSRKYRAPATTPMERARRMRFLQSRGFSHDTIRRVVSSRDDDDALNP
                     "
     gene            complement(18637..19719)
                     /gene="recA"
                     /locus_tag="AC16O9_03375"
     CDS             complement(18637..19719)
                     /gene="recA"
                     /locus_tag="AC16O9_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6515690.1"
                     /translation="MDENKSKALAAALSQIEKQYGKGSIMRMDSEAIKDVQVVSTGSL
                     GLDLALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKMGGTAAFIDAENALDPQY
                     AKKLGVNIDELLISQPDTGEQALEIADMLVRSGAVDVIVVDSVAALTPKAEIEGEMGE
                     PQMGLQARLMSQALRKLTANIKRTNTLVIFINQIRMKIGVMFGNPETTTGGNALKFYA
                     SVRMDIRRIGSIKKGDEVIGSETRVKVVKNKVAPPFRQAEFDILYGGGISREGEVLEL
                     GVAHGVVEKSGAWYIYKDDRLGQGKDNCRDFLKENPELAREIEIKIREKAGIAMPLPV
                     VPAANEAEGEEEHAETVVRAPARKRG"
     gene            19889..20764
                     /locus_tag="AC16O9_03380"
     CDS             19889..20764
                     /locus_tag="AC16O9_03380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515691.1"
                     /translation="MAKVAFLGLGVMGYPMAGHLKKKGGHEVTVYNRSAAKAQQWVKE
                     YGGQSAPTPKEAAQGCDFVMMCVGNDDDVRAVAYGDSGALAGLKKGAILVDHTTASAV
                     VARELHAKCKEKGVGFVDAPVSGGQAGAVNGQLGIMCGGEQADFDKAKPVIDVYAKMA
                     ALIGAPGAGQLTKMVNQVAIVGLLQGLSEALNFGKNAGLDMEKVVAVISKGAAQSWQL
                     ENRWKTMAEGKFEGFGFASDWMRKDLNIALAEAKKNGSYMPGTALIEQYFGRISAAGH
                     GRWDNTALISLLKKS"
     gene            complement(20761..21783)
                     /locus_tag="AC16O9_03385"
     CDS             complement(20761..21783)
                     /locus_tag="AC16O9_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6515692.1"
                     /translation="MSFKLGVTSDILDARGEPVFGREPLEALDAATGLEWEWLPRGIR
                     EITADFAEKYDALYVNSPKVPAAAVAGKDLRLKVVSRHGVGYDSVDVAAMSKAGVLVC
                     NTPNAMPRPVATMALTLVLALAHRLMAKDRMTREGRWNDRVEHMGMGLTGRTLGVVGA
                     GRIGKELLRMAKAFDLRLLAADPYADELELGYIGARKMPLETLMAEADFVVVTALLNE
                     ETRHLVNARQLARMKSSAYLINVARGPVVDEKALYAVLAAKKIAGAALDVFEEEPTPA
                     DNPILRLDNVIVTPHSLCWTDELFANIARTAVGAVLAVHAGRAPRFVVDAAALGHPRV
                     RGWLRA"
     gene            complement(21780..22553)
                     /locus_tag="AC16O9_03390"
     CDS             complement(21780..22553)
                     /locus_tag="AC16O9_03390"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR6515693.1"
                     /translation="MRENAVKNKLAAGGTALGSMIFEFFTPGMPRLLKNAGAEYALYC
                     MEHTGASFETLKPQFALCRALGVVPMVRVPGTEYDFIARALDCGALGVMVPLVDTAEQ
                     AQFIVSCTRYPPAGGRRGAAFGFAHDDYEGGDVVDKMKLIHERTLVIAMIETKSGLEN
                     VDAIAAVPGVDALWLGHFDLSNFLGIPGQFGSPVFQDAIRRIVAAAKKHGKAAGYMAA
                     DAALGKEYLDYGFRMIATGTDQGMLQAAIRASLAAMRKA"
     gene            complement(22562..23797)
                     /locus_tag="AC16O9_03395"
     CDS             complement(22562..23797)
                     /locus_tag="AC16O9_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515694.1"
                     /translation="MTSVAGSGRWLALLCASRVLSAAWFVAYSAVLPLVREDWGLSAR
                     DAGAIQGAFHLGYLSSLFLVGFIADYFGAKRAFLVTGIAGALSPIAFALFVEGFWSAL
                     WLHALTGLTQGGFYTPMLALVNEHVAANRRGRAMGLMIAASSAGYALALGVAGLVVSV
                     AGWRAALGAVAAMPLASWLIALAATRATPNVVHPRPEGHTVLASIPAVLKNRRGMLSV
                     WGYTFHSWELLGLWAWMPTFLTAALVAGGSSGAQAAALGLSLSALTYVANIGGSITGG
                     IMSDRWGRTQTILLWSCVSLALSFSIGWLIALPVALLVALACLYNFAGIADSSVHSTV
                     IAESVPPHILGAAYAVRSVVGFGTGVVSPVVFGWALDLAGGGKMSGDAFAWGVAWATL
                     GLGGLLGPLATWKLQRLQR"
     gene            complement(23794..24891)
                     /gene="amrS"
                     /locus_tag="AC16O9_03400"
     CDS             complement(23794..24891)
                     /gene="amrS"
                     /locus_tag="AC16O9_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR6515695.1"
                     /translation="MDAARAALETSHPGRWWHALADGRVQCDLCPRDCRLHEGQRGLC
                     FVRMMEGGRMVLTTYGRSSGFCVDPIEKKPLNQFYPGTAVLSFGTAGCNLACKFCQNW
                     DISKSRELDRLQDAAAPEAIAKAAVQMGCASVAYTYNDPVIFAEYAMDIADACREQGV
                     KNVAVTAGYIHAEPRREFYAKMDAANVDLKAFTDEFYFKLCGAHLQPVLDTLAYLVKQ
                     TEVWTEITTLLIPGKNDSDEELEAESKWIYENLGPDVPLHFTAFHPDWKMNDIPATPT
                     ATLTRAREIALRAGLRYVYTGNVHDAEGGTTFCPSCGEAVIVRDWYRINRYRLSEAGA
                     CLSCGAQLPGRYRKFTKPFGPRRIPVRLAAA"
     gene            complement(24867..26231)
                     /gene="amrB"
                     /locus_tag="AC16O9_03405"
     CDS             complement(24867..26231)
                     /gene="amrB"
                     /locus_tag="AC16O9_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR6515696.1"
                     /translation="MSQIRPAAVAGTFYPRDARALQREVADLIDGVENLAPRFGHPKA
                     LIVPHAGYIYSGAVAARAYDELAAARGIVGRVVLLGPVHRVPVRGLALPGVEAFDTPL
                     GRVPLDADALRMLAPFKQVVTSAAAHAAEHSLEVQLPFLQKMLGKFALVPLAVGDARP
                     QEVRDVIERLWGGPETLFVVSTDLSHYHPYDEARAIDQATLARIAAFDTDINHEEACG
                     ATPLNGFLATAKARGLSIRLLGACNSGDSAGGRDRVVGYSAFALYEGERVTLQDAGLA
                     LLAIARAAIGHALGVYAEPPRTEVAPWLARPGATFVTLRLDGKLRGCIGSLAPTRSIG
                     LDVAENALGAALRDPRFAPLTREEWSRCQVEVSVLSPPKPLRFADEAELLAQLRPGED
                     GVILEYEGKRGTFLPQVWEGLPETRRFLEELMRKAGIPADTRLARCKLWRYRVIKWTQ
                     PAPH"
     gene            complement(26228..26695)
                     /locus_tag="AC16O9_03410"
     CDS             complement(26228..26695)
                     /locus_tag="AC16O9_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR6515697.1"
                     /translation="MNDLARGIGEALKARGERLATAESCTGGWVAMALTSIPGSSDWF
                     ERGYVTYSNDAKREDLGVAEATLRQYGAVSEQVAREMAAGAARRARVQVALAVTGVAG
                     PGGGTRDKPVGLVCFAWAHGSKITSETRRFDGDRDSVRRQAVVHALERVRELL"
     gene            complement(26692..27183)
                     /locus_tag="AC16O9_03415"
     CDS             complement(26692..27183)
                     /locus_tag="AC16O9_03415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR6515698.1"
                     /translation="MMVFRPSARFVFTHPAHFIAFGFGAGLSPFAPGTVGTLIALPIW
                     WLIGPAYPPLVLLAILAFLFLAGVWACDITGRHLGIADHGGMCWDEVVAFLLVLAIVP
                     QERHWQAAAFLLFRLFDVVKPPPIRQLEKRMKGGFGVMFDDILAAGYTLLVLALAKRL
                     LFP"
     gene            complement(27180..28097)
                     /gene="thiL"
                     /locus_tag="AC16O9_03420"
     CDS             complement(27180..28097)
                     /gene="thiL"
                     /locus_tag="AC16O9_03420"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR6515699.1"
                     /translation="MPSEFEIIRRYFSRPARNATLGVGDDAALLAPAAGCELAVSTDL
                     LLEGRHFAARAEPRALGHKALAVNLSDMAAMGASPRWATLALALPRADEDWLAAFAEG
                     FYALATRFKVDLVGGDTTRGPLSICVTILGDVPAGAALRRDGAKPGDDVWVSGELGGA
                     AFALEHPENAKAAARLHLPEPRVALGERLRGLASAAIDVSDGFAQDLGHILERSGVGA
                     AVRYADLPKFRIGDAALEARCALSGGDDYELAFTAPAAARAAIGQLAAPLGVALTRVG
                     SIHAGAPRLRLLDPQGREIEVAAGFDHFA"
     gene            complement(28106..28543)
                     /gene="nusB"
                     /locus_tag="AC16O9_03425"
     CDS             complement(28106..28543)
                     /gene="nusB"
                     /locus_tag="AC16O9_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6515700.1"
                     /translation="MKAAKGPRRRSREIALQALYAWQMGSGDALEQVRAIDDDGRTDR
                     DLAEALVRGVQARAAELEGLIAPCLDREFRRLSPVERAILYIGAFELQAHPETPFKVV
                     INEAIELGKSFGGAGGHKYVNGVLEKLARGLRAQETAGGRQPA"
     gene            complement(28540..28959)
                     /gene="ribH"
                     /locus_tag="AC16O9_03430"
     CDS             complement(28540..28959)
                     /gene="ribH"
                     /locus_tag="AC16O9_03430"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR6515701.1"
                     /translation="MRLGIVASRFNDALSGKLLQRARDEAKKLGVHELTVEEVPGALE
                     IPLALQWLAQSGKFDCLAAIGCVVRGDTYHFEVVANESARGVMDVALEFGIPVANGIL
                     TTDDEKQAEARLDKGAEAVRVALEMARLRAKVAAWPK"
     gene            complement(28964..>29781)
                     /locus_tag="AC16O9_03435"
     CDS             complement(28964..>29781)
                     /locus_tag="AC16O9_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR6515702.1"
                     /translation="IATGISAADRALTIKVAAAPEARAEDIVQPGHVFPLVAQAGGVL
                     VRAGHTEACCDLARLAGLAPAAVLCEVMKDDGTMARLPDLLEFCAEHGLRIGTIADLI
                     EYRSRHESLVTRGLARDIDTAWGRFHLVTFHDVPTRGTHLALVVGTPAREAETLVRVH
                     EPLSVVDLLDAGPGTHTWGVGEALTAIAKAGTGVVVLLNCNLASDAIDARLAETRPAR
                     TGRSADLRTYGIGAQILRELGVGRMRLLAAPRKMPSMTGFGLEVTGYVESPRG"
BASE COUNT         4696 a        10506 c        10275 g         4304 t
ORIGIN      
        1 cacaaggacg acctgcacgc cgcccgcgcg tgcgggttgc ggaccgcgta tgtcacgcgg
       61 ccgcgcgagt acggctccgc gaagaaggcg gacctttcgc gcgagcccgc cttcgacgtg
      121 cacgcgcgcg atttcaacga cctcgcccgc cagctggcgc gctgaccatg ctcgcgaaga
      181 agaaatcacc cgccccgcgc caccgcctgc tgatcgtgga cgacgaggag tcgatgcggc
      241 tgctgctcgc gcgccaggcc tcgaagggcc tgaaggccga ggtgcagctt gcgggcacct
      301 gcgagcaggc gctgcgcctt gcgcgcaact acgcctacga cgcgatcctg ctcgacctga
      361 tgatgccggg gatcggcggc ttcgaggtgc tgaaggcgat ccgcgcgtcc tcggccaaca
      421 tggcgacgcc gatcctcatc atatcggcgc tggaagacct ggccaccaac gaccgctgcc
      481 tcgcggccgg cgcgagcggc ttcctggtga agccggtgga gcagcgcgcg ctggagatcg
      541 cggtgaaatc cctgctcgag gcgcgcggca aggcgatgaa tacctaggag aacgcatggc
      601 gcagatcaag atcggcaacg tcgaggcgct gggcaagccg ctcggccagt actcgcacct
      661 cgcgcgggtg aagaacgcga ctgaaacgct gtacatcgcg ggcatgctcg cgccggggga
      721 cgacttcgac acccagtgca gcggggtgtt caggcaggtc gaggcggcgc tgaaatccgc
      781 gggcgcgggc tgggccaatg tcgcgcagtt caccacctac ctggtgcact cgcaggacat
      841 cccgaagttc atggcctggc ggctgcgcga gttcccgaag ctgtttcccg acgggaagta
      901 cccgcccaac accctgctcg tcgtcgaccg cctcgtccag gagccgttcc tgatcgaggt
      961 gcaggccatc gcggcgctct agcgcctagg gctcctcgac gaagacttct tcgcgcttct
     1021 tgcggatgtt gggcagcgcg atgatcacca gcagggccac cgccgcgagc aggaacgaaa
     1081 acgagatcgg cttggccatc gggttgaaaa acaccgtcgg atcgccgcgc gacagcagca
     1141 tcgcgcggcg caggttctcc tccatcagcg gtccgaggat gaagccgagg atcatcggcg
     1201 ccggctcgca ctcgagcttg acgcagacgt agccgaacac gccgaacgcc gacatgatca
     1261 ggcagtccca gggattgttg gagaggctga acacgcccac gttcatgaag atcaggatcg
     1321 agggaaacag gaaccggtag ggcacgcgca gcagcttgat ccacatgccg atgagcggca
     1381 ggttgagcac gacgagcatg gcattgccga gccacatcga gacgatcagc ccccagaaca
     1441 gctgcggctt gtcggtcatc acctgcgggc ccggcgcaat gccctggatc atgagcgcgc
     1501 cgatcatcag cgccatcacc gcgttgccgg gaatgccgag cgtcagcatc gggatgaagg
     1561 aagtctgcgc gccggcgttg ttggccgatt ccggcccggc gacgccctcg atcgcgccct
     1621 tgccgaactg ctccgggtgc ttcgagatct tcttttccac cgtgtaggac gcgaaggacg
     1681 acagcatggc gccgccgccc ggcaggatgc cgaggcacga gcccaggccg gtgccgcgca
     1741 ggatcggcgc ccaggagcgc ttcaggtcct cccgggtcgg gaacaggtgc gtcagcttgc
     1801 cggtgaacac ctcgcgcttt ccgcgctgct cgaggttggc gacgatttcc gtgacgccga
     1861 acatgcccat cgccacgatg acgaagccga cgccgtcggc gagctccggg atgccgaagg
     1921 tgaagcgcag cacgcccgaa ttgacgtcgg tgccaaccag gccgagcagc aggcccagca
     1981 ccaccatggc gatcgccttg acgagcgagc cgtgcgcgag cacggtagcc gccaccaggc
     2041 cgagcaccat cagcgaaaaa tactccgccg gtccgaactt cagcgccact tcggcgagcg
     2101 gcggcgcggc ggtggcgatg atcagcgtcg ccacgcagcc ggcgacgaac gagccgatcg
     2161 ccgagatggc gagcgccggc ccggcgcggc cctggcgcgc catctggtag ccgtcgagca
     2221 ccgtcactac cgaggacgcc tccccgggga ggttgacgag gatcgcggtc gtcgagccgc
     2281 cgtactgcgc gccgtagtag atgcccgcga gcatgatcag cgccgcggtc gggtccaggt
     2341 tgaaggtgat cggcagcagc atggcgatgg tcggcaccgg gccgatgccg ggcagaacgc
     2401 cgatcaacgt ccccagcagc acgccgagga ggcaatacag gacgttctgc caggaaaagg
     2461 cgacctgcgc gccgaggatc aggttgtcga ggacttccat cgccctagcg tatcgggcag
     2521 ttctcgatga acgacgggca catcgggaac ggcagcgtca ggcccttcac gaacacgagg
     2581 tacgaaaaga tcaccagcac cagggcgacc acggtcactt ccttccactt gaactcgtgg
     2641 ccggcgagcg cgccgatgaa caccgcggcg gcggtggcga tcgcgaggcc gcccggctgc
     2701 atcaggtagc cgtaggcgag caccgagagc gtcacgagga cgagcggctt gaagtgaaac
     2761 gcggcgactt tgggtccgtc cagtgccagc gactgcagca ggacgatggc gccgagcagg
     2821 atcagcaggc cgccgagcat ggcggggaag tacccgggcc ccatgcgcac cgccgagccc
     2881 atctggtagt gcgcgaacga ccatgcgacg aagaacaggc cgaaacccac gaacatcatg
     2941 ccggcccaga aatccctcgg gctcctgatc ctcaccggat ttcgctctcc cccgtggtag
     3001 cgcgcgggag tgtagcatgc agcgcgtgcc cgttttccgc gcgttcctcg tttccgtcgc
     3061 gctgctcgcc gccgcctgcg gcgatgaacc gaagctcgcg ccgctcgcgc ccgacgcgct
     3121 ggtgctcgcc ttcggcgaca gcctcaccta cggcaccggc gcgcgcgaag ccgagagcta
     3181 tccggcacag ctcgaggctc tgatcggacg ccgtgtggtg cgcgccggcg tgcctggcga
     3241 agcgaccgcg caagcactca agcgcctgcc gggggagctc gacgaatacc agccgcagct
     3301 gctcctgctg tgcatcggcg gcaacgactt tctcaggaac ctgggcaagg cgcaggccgc
     3361 ggccaacgtg cagaccatgg tggcggtggc caggcagcgc ggcatcgacg tgctgctcat
     3421 cggcacgccg gaaaagggcc tcaccgtgac gccgcccgcg ttctacgccg agatcgcggc
     3481 acagcaccgc atcccctacg agggccgcgt gatcggcgag atcctgcgcg actccgagct
     3541 caagtccgac accatccatc ccaacgcacg cggctaccgg ctgatcgccg agcgcgtagc
     3601 ggcgctactc aggaagtcgg gcgcgctctg aactcgatgt cgccgtagta ggcctcggcg
     3661 cgctcgccgg tgttgtcggt atcggtcgac accactaccc cgctcaccgc cggcggctcg
     3721 gcgccgaagg cgcgccggta gtcggcggcg acgtcgcgct cgtagtcgct ccagcgcccg
     3781 agctgcgcgg cgccgctctc gaccacgatc atctgcacgc ggccggtgta ggcgttcggc
     3841 acgaccgtgc ccacggtcgc gttcgaggcc cagacgtagc acagtgccgc gctcggcacg
     3901 tcgtcgccgt agatgacgcg cgccagcgtc acgccgaagc gctcgccgaa gggcagcgcc
     3961 gcgagccgca ggtcgaaggt gacgtacagg cgcgcggcgt agtcgtcgcc cgacttgctg
     4021 cgcagatcgg atttctcggg cagccgcgtc gccttccagc gccacgcgag caccggatgg
     4081 gtcgcgggat cgaccttgag gtcgcggatg atgcccgcgg tggaggcctc agcgcgcgcg
     4141 cgaaggaccg tgcggccggc gtcctccacc aagtcgtatt gcgtgcgcct gtcctgcccg
     4201 gcgaaggcgt aggcgcggta ctcgggcggc agccgcgcgc cggccgcgag cgctgagaag
     4261 cgcatcgcgt acaccggctc cgccgcacgg agcgtccccg gcagcaagcc aagcgccgcc
     4321 gcggcgagaa agcggcgccg cttcaccgcc gcagctcgcg gagccgcgcc acgtccgcgg
     4381 gccggtccac gtcccacacc tcgggcagct cgcgccagcg ccagccgagc gccgcgaggc
     4441 gcgcgcgcgt ctgcgccatg acctgcggcc cgccccactc gatgccctcg aacagccgcg
     4501 gcgtcaggcg ccgcagcgcg acgagcgcgt agccgccgtc ctcggcgggg gcaaacaccg
     4561 cgtcggcgcc gcccgccagc cagcgcgccg cgcggcgcag gtgcgcgggc cgcagtgccg
     4621 gcgcatccgc gcccatgacg atgacgcgcg gcgcccggcg caggttgcgc gcgaatgcat
     4681 ggtgcatgcg cgcgccgaga tcggggccgc gctgccgggg cgtggaccag agcgtcacct
     4741 cgcccaagcc cgccgcgcgt gcggtggcga gcgcgcggcg caccagtcgc gcgtgcaggc
     4801 gcgccgcgcc atgcgcgccg aggagtggaa tcagccgcgt ctttacgcgc ccgggccgcg
     4861 cctcgcgcgc gaagacgagc accctagtgc gaatagcggc gcgccagctc atccggatcg
     4921 gctccccgcc agtagtcgta gcgcaggcgc cacatgagga gcacggtgcg cgcgacgccg
     4981 cggcgctccc agcgccgcgc cgaggtgacc acgcgatcgg cgaggcaggc cggcggcgac
     5041 acccgcttca tcgcgcgact gaaggcaacg tcctccatca gggcgatcgg cggatagccg
     5101 ggaaaggcgt cgcggcgcac gaagatcgcc tggtcgcccg tggcgatgcc ggacaggcgc
     5161 gagcgacggt tgatgaagaa cgcgatcacc gcaaggcgcg ggtcgtcgga atcgatcgcc
     5221 acgtcgaagc ggccccaggc gtgatctgcg agcgcagcgc ccaccagccg gtccgcgtcc
     5281 ggcggcaagc gcgtgtcggc atgcaggaag aggagcgcat cgcccgcggc cgcggcggcg
     5341 cctgcgttca tctgcgccgc gcgtgcgcgc ggcgcggcga gcacgcggtc ggcgagcggc
     5401 acggcaagcc gcgccgtcgc gtcggtgctg ccgccgtcca ccacgatcac ctcgttgccg
     5461 cgcgcgcgca gcggcgccag tgccgcgagg caggcctcga tcccggccgc ctcgttcagc
     5521 gtggggacga cgaccgtaag ccgttcagcg accaatcgta atcgaggaag tcgaccggca
     5581 ccgggccgcc ggggtagccc agcaccttcg cgtactgcgc gaagtacttt tcgtacggca
     5641 cgaagtcctc ttcgaaccag tcgaagatct tcgagacttc cagccgcccg tcgcggtagc
     5701 ggttcctcgt gcggtcggag aggaagcgca ccgcctgctc gtcgagctgc gcttcgagcc
     5761 gcccggccac gtaggcctcc tcgcgcagca tcgggcagcc gatcgaagcg cagttgacgg
     5821 catagtgcac gcgcggatcg tcgtaggcgc cgggtctcct gagcgtgccg tgctcgatgc
     5881 cgtcgagggt catcggcttg ccaagcagcc tgaagaagtc gtccttgaag gggttgccga
     5941 agatcttgcc gaagtcccag atcgagcgga tgtcgggcca gcgcgtgagg atcttctcga
     6001 cggtaaacgc gttgtaggcg ttgatcaggt acgccttcct ctcattcttc ggccaggcgt
     6061 cgaactcggc tttcggcacc ttgctgaggc cgtcgaggta ggctttgagc gcggcacggt
     6121 cgcctgccat gccggcgtaa tccatttgcg aggcatgctg gccgggcagc actttcacgt
     6181 gccttttcac cagcgcatcc caggcggcgt gcgaatggtc gaacgccgcc tgtgccggca
     6241 gcgccgcgaa agccgccgtg aggacgatca gccgcgcatc cacgcgtgga acctctcgac
     6301 ataggtcagc agcctctcgg gcgcatgcgc ccgcttccac acgccggcgg cgtacttgtt
     6361 ggcttcggcc agcgtcgggt agatgtggat ggtgccgagg atcttgttca ggccgatgcc
     6421 atgcttcatc gccgcgacgt actcggcgat caggtcgccg gcatgctcgc cggcgatggt
     6481 gacgccgagg atgcggtcct tgcccggcac ggtgagcacc ttcaccaccc cgtgcgcctc
     6541 ctcgtcggcg atagcgcggt ccagatcatc gatcccatag accgtgacct cgtgcggaat
     6601 gttccgcgct ttcgcctcgg tctcgttcag gccgacgcgc gccacttcgg ggtcggtgaa
     6661 ggtcgcccag ggaatcaccg agtagtcggc gcggaacttc ttcaggctgc caaagagcgc
     6721 gttcacggtc gcgtaccagg cctggtgcga gcccgtgtgc gtgaactgga acgggccggc
     6781 gacgtcgccg gcggcgtaga tgttcggata cagggtctgc agatactcgt tcgtctccac
     6841 cgtgcgcgcc ttggtcaccg ggatgccgag ctcctccagc ccgtagcccg cggtgttggc
     6901 gacccggccg accgccacca gcagcgcgtc gaactcgatc gccaggtcct tgccctggtg
     6961 ctcgcagatg agcaccttgc gcccgtcctc gacgcggaac tccttcgcct tgtggccgag
     7021 gcgcacgtcc acgccttcgg cgcggaagcg gctctccacc atcgccgcga tctcggggtc
     7081 ctcgcggatc aggagccgcg gcagcatctc cacctgcgtc acctgcgcgc cgaggcgctg
     7141 gaacgcctgc gtgagttcgc tgccgatcgg cccgccgccg agcaccacca gccgtcgcgg
     7201 cagctcgcgc agctcccaca ccgtgtcgct ggtgtagtag ccggtcttct cgatgcccgg
     7261 gatcggcggc acgaacggcc gcgcgccggc ggcgatgacg atggagcgcg tcgtcagcac
     7321 ctgcgtcgac ccatccggct tttgaatttc aacgatccag ggcgaggcga tctttgcctc
     7381 gccctggaca cactcgacgc cgagcccttc gaagcgctcg gcagaatcat ggggttcaat
     7441 ggtgcgaatc acccgctgca cccgctccat cacgtcggcg aagtcgaact cggcgcccgc
     7501 attcgtcagc ccgtactcct tggcgcgccg gatcgtcgac agcacgcgcg cggtcttgat
     7561 cagcgccttc gagggcacgc agccggtgtt caggcagtcc ccgcccatct tgtgcttctc
     7621 caccagcgtc accttcgatt tcaccgcggc ggcgatgagc gacgccacca ggcctgcgga
     7681 gccggcgccg atcaccacca ggttgcggtc gaactgcttc ggcttctgat cggcccagcg
     7741 cgcgtagacg cggcgcgcct tgaccgcgtc gagcgccttc ttcgccgcga gcgggaacag
     7801 gccgagcagc gtgaacgcga ccaccagctc cgccgacagg cggaactcgg cgagctgggt
     7861 gcccgcgtac acgtacacga gggtgccggc gagcatgccg agctggctca cccaatagaa
     7921 ggtccaggtg cggatgggcg tgaggcccat caccaggttg atgacgaaga acgggaacgc
     7981 cggcacgagg cgcagagcga acaggtagaa cgcgccctcg cgcgccacgc cgtcgttgat
     8041 cggcttcagc ttgtcgccga aacggccctg cacccagtcg cgcagcagga agcgcgacac
     8101 gaggaaggcg agcgtcgcgc cggccgacga ggcgaaggat acgatcaccg tgccccacag
     8161 caggccgaac accgcgccgc cgaaaagggt catcaccgcc gcgcccggca gcgacagtcc
     8221 ggtgatggtc acgtagacga ggaagtagcc gagcgcggtc tgccagggat gcgacaggaa
     8281 gtaggcctgc gccgcatcct gctgcgcacg cagctgctcg agcgtcaggt agcgctcgcc
     8341 gccgagcgcg aagaacgcgc cgaccagcac gacgacgatg gtgaggacga gaaggcgggt
     8401 gcggttcacg cggtttggtc gcgtcgggcc ccgcccggct tacaacaagc cctgggcgcg
     8461 ctgtccctgc tcgtcgagga gctgcatcag gcggtcgctg gtggcgcgca ggcgctgcga
     8521 cagcatcagc accagctcca tcaggatctt cgcgccgagc atcggctgct cgacgatgat
     8581 gcgcgccagg ctctcgcggt cgagcaccgc caccagcgcg ccgtccgccg cgacgcaggt
     8641 ggcgaagcgc gccgcgccgt cgatcatcga catctcgccg agcgtgcgtc ccggctcgac
     8701 cgtcgcgatc acctgcggcg tgttccagcg gtcgagcttg cgcacctcga tgcgcccctc
     8761 gatcaccatc atcatgaagt cgccgccgtc gccctcgcgg atgatctccg cgcccgccgg
     8821 cgcgcggtac acgtccatga agtgcgacag catgcgcacc tcgtcgggcg tgaagttctc
     8881 gagcagcggg cactgcggaa tcaacgcgtg cagctggtgg gcgaaacgcg ctgcgtcgcc
     8941 gaggtactcg agatgcgcga gctgctgcgg gcgtccggtc atgaggagcg gagcattcta
     9001 gccgatcagg aaagacaccg tcgccaccca gcccagggcg gccgcagcga gcaacgggtc
     9061 gcgcagcagc tcctgcgccg ggtcgccgcc gcccccgcgg tagtgcaggc gaaacaggta
     9121 gcggaaggtg ccgagcagca cccagggcag cgaccagatc aggcgctcgg taccgtgcag
     9181 cgccaccgtg gtctcggcga ccgtatacag cgcgtaggtg accaccatgc cgagcgcgca
     9241 ggcgaacacg gcgcggtcga ggaacgcgag gctgtagtcc tcgagcacgg cccggtgctg
     9301 gccggcgtcg tcggcgagac gatcgagctc cgcgcggcgc ttggcgaaac cgaggaacaa
     9361 ggtgaggaga aacccgcagg cgagcagcca gcgcgaaggc tcgatgccga tgccccaggt
     9421 gccggcgagg atgcgcagca tgaagcccgc ggcgatgatg aacacgtcga gcaccgcgac
     9481 gtgcttgagg ccgaggctgt aggccgcggt gagcacgatg taggcggcga cgatcgccgc
     9541 cacgcgcacg ccggccgcga aggcgattcc cagcgccgcc accgcgagca gcagcgcaat
     9601 cccctgcgcg gcacgcggcg tgatcgcgcc acgcgccacc ggccggttgc gcttctccgg
     9661 gtgctcgcgg tcgtgcaccg cgtcttgcgc gtcattgaac gcgtagacgg cgctggaggc
     9721 gaggcagaag gcgagcgcgg cgacgagcgc cgcagcgacg tagtgcgggt caccccagcc
     9781 gtggccgaag agcagccccg tgagcacaaa gccgttcttc acccagtggt gcggccgcag
     9841 cagcagcagg aggtcgcgcg cgctcaccgc ggccgctcga aaccgtagcg ctcgaagtag
     9901 tagcagccgc cgaccggcag ccaagtcgga atgcggtacc agcgcttgct caccaccgcc
     9961 agcacccgct ctcggtaggc ggcatagtcg aagccgcgcc cgagcaccgc gtaatgcgtg
    10021 acgccgcgcg cgctcgcctc gcgcgcctcc acttcgcgga agtacggccg gtacgcatcg
    10081 agctccggcg gctcgcgccg gaacacgagc aggtcgcgcc cgtccaagcg gcgccagtcg
    10141 gtgaggatgt cgtcatggcg cgcgtgcgag gagccgccgc cgaacacggg cacgcggcga
    10201 cgcgtatgaa aagcaagctg cgcggccacc gagtagccct ccgtgaccag gcggtagcgc
    10261 gccatgagcg gaccgaaagg ctcgagcacc tcgcgcggct cgaacagcag cacgaggcgc
    10321 tggtacagcg gcgtgtcgcg ccacgtttcc aggggcggca ccagcagccc gatgagcagc
    10381 gctgcgtgca gcacggcgag cgcgccgaac cagcgcagcg tgacgacgag cgcgcggcgc
    10441 tccagtccga gcgcgaggct cagcaccaga gccggcacga aggcgagcag ccagtgcagg
    10501 ccgatgcgct tgaccggcga gagcagcgcg aacagcgcga gcggcaccag ccacgccgcc
    10561 accgtgaccc cgacttcgac gcgcggcagc gccgcgcgca gccgcgcgcg ctccttccag
    10621 gcgaaccaga ggagcggcgc ggcgaggtag gcgagcgacg cggcatagag cgccggcgtg
    10681 gtccacgaca gccccgcgtc gtcgtggcgg ttgatggcgt tgaacatcac gttgcaccag
    10741 cacgcctgcc agttccagta gagattgagc aggcccgccg gcagcgcgcc gaggaacgcc
    10801 aggccgaagg cgccggggcg gcgtgcggcg attgcccacg ccaggtagcc caggccgagc
    10861 agcacggcga agtacttcga caggaacgcg aagccgagga gaacgccggc gagcaggaag
    10921 cgctggcgcg cgaaagcgag gagcgaggcg accgcaaaca gcgcgagcgg cgtatcggtg
    10981 gtgatgaaca cgttccatac gttcatcggc accagcagca ccgcgagcgc cgcagtatcg
    11041 gcggtgtctg cgtcgcgcgc gaaccaggcg ctgagcgcgt ggcgcacgag gagcgcgagc
    11101 gtcgccggca gcaggatcga gggcaggcgc acaaaccact cggcatgcga aaccgcgacc
    11161 agcggcgcga gcagccagcc caccatcggc gggtggtcgt agtaccccag gtcggggtac
    11221 cggccccaca gcgcgaaata ggcttcgtcc gacgtgatcg gcagggcgga gccgagccag
    11281 aagcggaacg cgagcgtcgc ggcgagcgcg agccagaacc attgccgcag cgtaagtgac
    11341 accacggcgg ggattctata gggacgccgc tagcggcgtt tcgcgtgctt cctgcgctcc
    11401 ttcaccgcgc cggcgaggct ctcgagcacc tcgatcgagt ccttccagcc caggcacggg
    11461 tcggtgacgc tctggccgta ctgcagcgcc ttgccgggcg cgagctcctg gcgcccttcc
    11521 accaggtgcg actcgaccat ggtgccgacg atgcgccgct cgccgcccgc gatctgcgcc
    11581 gcgagctcgc gcgtcacgtc gagctggcgc ttgtactgct tggcggcgtt ggcgtgcgag
    11641 cagtccacca tcaggcgctc cgggatcttc gccttggcga gctcggcgca ggccgcggca
    11701 acgctcgccg cgtcgtagtt cgggctcttg ccgccgcgca ggatgatgtg gcagtcgtcg
    11761 ttgccgcgcg tttgcacgat ggccacctgc ccggtcttgt gcaccgagag gaagtggtgc
    11821 ttctgtcccg cggcgaggat cgcgtcgacc gcgatcttca cgttgccgtc ggtgccgttc
    11881 ttgaagccga ccggggccga caggcccgag gcgagctcgc ggtgcacctg cgactcggtg
    11941 gtgcgcgcgc cgatcgcccc ccaggacacg aggtcgccca ggtactgcgg cgagatcacg
    12001 tcgaggaact cgcagcccgc gggcacgccc gcctggttga tgcgcacgag aaggtcgcgc
    12061 gcaatcctga ggccctcgtt gatgcgaaag ctgccgtcga ggtacgggtc gttgatgagg
    12121 cccttccagc ccaccgtggt gcgcggcttc tcgaagtaca cgcgcatcag cagctccagc
    12181 tcgccctcgt gcaggcggcg ctcggcggcg agccgcccgg cgtactccag cgccgccttc
    12241 gggtcgtgga tcgagcacgg gcccatcacc accagcaggc ggtcggactt gccgtgcagg
    12301 atctcgcgca cccggcgccg cgtgtcgctg atcagcgcct ccaccggcgt gccctggatc
    12361 ggaaaaaagc ggatcaggtg ctcgggcggc ggcagcggga cgatgttctc gatgcgctcg
    12421 tcgtcggtga gcgaggtctt gtcggcggga acggaaccga ggaaaggatc gatgggcgtg
    12481 gtcatggtgg gctctcggaa ataaaaaaac cgccaggcat ctctgctctg gcggtttctc
    12541 gggttcgcgg gttgcgcggc tagccgatct cctccgccag agggcgcgga aagctgaaga
    12601 agaagccgaa gtaccagcgg gtcatgggcg gggattgaag catgggtgac gcaaattgtc
    12661 tactgcgcgg gccacaggac catctgcgtg cagcggaaag tcgcgatcct gcgctgcgcc
    12721 ccctccacgc tcacctcggc gtcccagacc tgcgtggagc gcccgaggtg caccggccgc
    12781 gcccggcaga cgatcgcccc gtcgcgcgcc gtgccgagga agttggcctt gagctcgagc
    12841 gtcgtgaagc cgctcgcgcc ttgcgggagc tgcgccacgc agccgtagcc gcaggaggtg
    12901 tcggccagcg cgatgactgt ggccgcgtgc aggaagccgt tcggcgccat gtggtccttg
    12961 cgcacctcga gccggctctc cactgcctgc gcgctcatcg tcaggatctg caaaccgagc
    13021 aggcccggca ggtggccgcg actgcggccg ttgaaaacct ccggcgtcag ttccggtctg
    13081 aaggaagaca tggtcgcaat ctagcactcg cgcgtacgga ctgccaaagc gctggcgcgg
    13141 gacttgctac gcttcaatgc gtctgctagc gttttcgctc gggtagcggt accggcgaaa
    13201 agaacagcag caaggaggca cgaatgtcgg caagcatggc tataccgatt gcgggcttca
    13261 aggacgtcta cgacgtgccc gccgtggaga aggcgcgaca cgggcttccc gccagcgcca
    13321 acgaggcgct gcgcgcgctc tacgacaaga tgctgcgcgt gggcgggcag cgcttcaccg
    13381 tcaagccctc ggcgatgccg ccgatgaatg agctgttcga ggacctgccc aacttcggca
    13441 gggtgctcga ggacatccgc aagcagctcg ctttgtgcat ggactccgag gatgcggtcg
    13501 agctgccgcc gatgctgctc cttggcgccc cgggcatcgg caagacgcac ttcgcgcggc
    13561 gcatcgcgca gctgctcggc accggctgcg gcttcgtgcc gatgagctcg ctcaccgcag
    13621 gctggatcct cggcggcgct tcctcgcagt ggaagaacgc caagcccggc aaggtgttcg
    13681 agacttttct gaacggcgag tacgccaacc ccgtgatggt ggtggacgag ctcgacaaga
    13741 ccagcgccga cggccagtac gacccgctcg gcgcgctcta cgggctgctc gaggtagaca
    13801 ctgccacgcg cttcgtggac gagttcgccg aaatcccgat cgacgcctcg ggcgcggtgt
    13861 ggttcgccac cgccaacgac gcctcgcgca tccccgagcc gatcctgaac cgcatgcacg
    13921 tctacgagat cgacccgccc gatgccgagg gtgcggcgcg catcgcgctc gccatctacc
    13981 gcgagatccg cggcgcgcac gactggggca agcgcttccc ggaggcgccg gcggccgcgg
    14041 tggtggacag gctcgccgcg ctctcgccgc gcgagatgcg ccgcgcggtg ctcgccgcgt
    14101 tcggcaacgc caaggtcgcc ggccgcgccg agctcagccc cgacgacgtc caggacgcac
    14161 gcggcccgcg gcggcgtatc ggcttctagg ctagcctcgt ttcagcgctc gggcagcagg
    14221 aagccgcagg cgagcacccc gagccacagc gcgagcgaca gcccgccggc cagccgcgcc
    14281 gccggcggcg gcatgcggcc cgcgtcccac gcggccgcgc tgcggtaggc cccggcgtgg
    14341 aagatcccgg cgttcacgcc cgcggcgagg atgaacccca tcttgagcgc gaacatgccg
    14401 ctgtcgatca gctcgcccgc atgcgcgacg aacatcagca ggccggccgg cacgatcacc
    14461 aggaagcttg ccgccgtcca gggcaggagt cgcccggcga ggcggcgtac cgacagcccg
    14521 cgccacagcc cgagcaggcg cagatcgacg acggcgatcg tccccaggag caccgcgacg
    14581 ccgccgaggt gcgcggcgag cgcggctggc ccgagccagc gcgactcctg catggcccgc
    14641 gcgagcgcgc ttgcggcaag cgcatcgatc gggctcatcg cgcaccctgg cgccgcgcga
    14701 gccagtcggc cacggcgagc ccggtgccga agtcccaggg ccgcagcttc tccggcgcga
    14761 tgcgccggta agccgccagc tcctcgttca gccgcaccgt gcccgtggcg ggcacatggt
    14821 aggcgatgat gagctcgttg cggtgcgcga acggatacag cccgacaagg tgcgcatcga
    14881 ggggatcgag gtcgagctcc tccttgacct cgcgcttcac gcccgcctcg ggcgtctcgt
    14941 ctttctccag gaagccggcc accaggccga acatcttctc cggccagtcc ttgttgcgcg
    15001 cgaggatcac ggcgccctcg tgctcgacga tcgccgccac caccggcacc gggttgtccc
    15061 agtgcacgaa gccgcaggca caggccggac gtgcgtcgcc ggcgcgcacc gcgagcggcg
    15121 ccgcgcacac cgggcagtac ctataggcgc tgctcaatcc aggcccggac cgactgcagg
    15181 gcggccggca gcttggcgct ctcggtgccg ccggcctgcg ccatgtcggg gcgcccgccg
    15241 cccttgccgc cgacctgctg cgcgacgtag ttgacgagct cgcccgcctt gaccctggcg
    15301 gtgaggtcgg cggtgacgcc ggcgatgagc gccaccttgc cgtcggcgac ggcgccgagc
    15361 acgatcgccg cgctcttcag ctcgcctttc agccggtcca ccgcctcgcg cagcgtcttc
    15421 gcgtccgcgc cgtccagcgt cgccgccacc accttggcgc ccttcacgtc gaccgcctgg
    15481 ccggcgagat cggtgccctg gccggaggcg agccgcgatt tcagcttcgc cagctcgcgc
    15541 tcgagcgact tcacgttgtc gagcacctgc gcgatcttcg attcgacgtc gtccaccggc
    15601 gcgcgcagcg cggcggccgc ctgccgcagc ttcgcctcct gcgcctgcac ccaggcaagc
    15661 gcgccttcgc ccgtctttgc ctcgacccgg cgcacgcccg cggctaccgc cgactgctcg
    15721 acgatcttga aaagcccgat gtcaccggtg cgcgcgacgt gcgtgccgcc gcagaattcg
    15781 cgcgagctgc cgatgtcgag cacgcgcacc tcgtcgccgt acttctcgcc gaacagcatc
    15841 accgcgcccg agcgcttggc gtcctcgatc ggcatgatct gcgccttggt cgacacgttc
    15901 cgcaagatct cggcgttgac ctgcgcctcg atgcgccgga tctcggcttc gctcatcggc
    15961 ttgtcgtggg cgaagtcgaa gcgcgtcttg tcctcgtcga cgagcgagcc cttctgctgc
    16021 acgtgagagc ccagcacctc gcgcagcgcc ttgtgcatga ggtgcgtcgc cgagtggtta
    16081 cgcatggtac gggcgcgaag ctgcgtgtcg accttcgcct ccaccgtgtc gcccgctttc
    16141 agcgtgccgg tcttcagggt gccctgatgg ccgaacacct cggcctggat cttctgcgtg
    16201 tcgccgacct cgaacgtgcc ggtggccgcc accagcgcgc cgcggtcgcc gacctggccg
    16261 ccggactcgg cgtagaacgg cgtctggtcg agcaccaccg tgcccgcctc gccgctcctc
    16321 agcgcctcga cctgggcgcc ttcgcggtac agcgccacca ccctcgcctg cacggcgagc
    16381 gtgtcgtagc cgcggaattc ggtcttgccg ccggcgtact ccagtcccgc cgcggccgag
    16441 aacttgctgg cggcgcgcgc gcgctcgcgc tgcgccgcca tcgcggcttc gaaccccgcc
    16501 atgtcgagca tcacgccgcg ctcgcggcag atatcggcgg taaggtcgac cgggaagccg
    16561 aaggtgtcgt agagccggaa cgcggtctcg ccgtcgagca gcttctgctc gccggaggca
    16621 agcgccgact cgagcacgcc catgccgttc tccaatgtct cggcgaagcg ctcctcctcc
    16681 tgcttcagca cggcgatgac gcgcgccttg tccctgctca gctcggggta ggccgcgccc
    16741 atcgccttgt ccaagtcctc gacgatgcgg tggaaaaacg gctgcttctg gccgagcttg
    16801 tagccgtggc gaatggcgcg ccgaacgatg cgccggagca catagccgcg gccttcgttg
    16861 ccgggaatca cgccatccac gatgaggaag gcgcaagcgc ggatgtggtc ggcgatgacg
    16921 ttgagcgagg ggttcttgag cggcttttgc ttcgtctcgc gcgccgccgc gttgatcagg
    16981 tcctggaaga ggtcgatctc gtagttcgag tgcttgccct gcagcaccgc cgcaatgcgc
    17041 tccaggccca tgccggtgtc gaccgagggc ttgggcagcg ggtgcatgac gccctggtcg
    17101 tcgcggttga actgcatgaa gacgaggttc cagatctcga tgtagcggtc gccgtccgcc
    17161 tcgggcgagc ccggcggccc gccggcgatg ccggggccgt ggtcgtagaa gatctcgctg
    17221 cacggcccgc acgggccggt gtcgtccatc tgccagaagt tgtccgactg gtacttctgg
    17281 ccgccgggct tgtcgccgat gcggatgcag cgctccttgg gcacgccgat ggtcttcgtc
    17341 cacagctcga aggcttcgtc gtcggtctcg tacaccgtgg tccagagctt ttccggcggc
    17401 agcctgtaga ccttggtgag cagctcccag gcgaactgga tggcgtctga cttgaagtag
    17461 tcgccgaagc tgaaattgcc cagcatctcg aagaaggtgt ggtggcgcgc ggtgtagccg
    17521 acgttctcga ggtcgttatg cgtgccgccg gcgcgcacgc atcgctgcga ggtggtggcg
    17581 cgggaatacg ggcgcttgtc cttgccgaga aacacgtcct tgaactgcac catccccgag
    17641 ttgacgaaga gcagcgtcgg gtcgcccgcc ggcaccagcg agctcgaggc gacgaccgcg
    17701 tgccccttcg actcgaagta ctcgaggaac ccggcgcgga tctcgttcga attcataggc
    17761 ccgctattct aaaggagcgc ccgcggtgcc cgggcaccgc gggcttgccc tacgccgcga
    17821 gccgaccgta cttcaccagc cgcgcccgaa tgccggtctg cgtgcgctgg tgcgctgccg
    17881 ccagctcgcc gagcgcgtgc ccggcgtcga acgcagcgag cagcctgcgg tcctcgtcct
    17941 cgctccacgg ctcgccggtc tttgccggca gctgggattt gcgccgctcg aactgcgcgg
    18001 cgcgctccag cgcttccatc gccgtgtaga gcgcccgcac cgtctgcggc cgctggtacg
    18061 cgccttcggc cggaaacact tccccggact ccggatcgac gccgttggcg agcgatctca
    18121 cgatcgccaa tgcctgtgcc tgatccatgc tgactcaccc ccttcccgcg cgagacgcgc
    18181 taggggttta acgcatcgtc gtcatcacgg gatgacacga cccgccggat cgtatcgtgg
    18241 gagaagccgc gcgattgcag gaaacgcatg cgccgcgcgc gctccatcgg cgtggtggcg
    18301 ggcgcgcggt acttgcgcga aagaatcgcg gtggcgcgca ctgcctcgtc ctcggccgag
    18361 acgcgctcga cgatgccctc gtcgacgccc ttggccttca gatcctgccg gatgcgcgcc
    18421 gcgccgtact tgcgcgcgag ctggcgcgcg cgcgcctcgg catagcgttc gtcggagagc
    18481 tgcttttttg cggcgagcgc gtcgaggagc tggccgaggg cttccggcga ctcggcgtgc
    18541 ggcgcgagct tgcgcgcaag ctcggcgcgc gagtgctcgc gccgcgcgag gtgtcgaagc
    18601 gcccgcgcct tgagctcctg cgcggtgtcg ggcgcttcag ccgcgctttc gcgccggcgc
    18661 acggacgacg gtctcggcgt gctcttcctc gccttccgcc tcgttcgccg ccggcaccac
    18721 cggcagcggc atggcgatgc cggccttctc gcggatcttg atctcgatct cgcgcgcgag
    18781 ctccgggttc tccttgagga agtcgcggca gttgtccttg ccctggccga ggcgatcgtc
    18841 cttgtagatg taccaggcgc cggatttctc gaccacgccg tgcgcgacgc ccagctccag
    18901 cacctcgcct tcgcgcgaga tgccgccgcc gtagaggatg tcgaactcgg cctggcggaa
    18961 cggcggcgcc accttgttct tcaccacctt gacgcgcgtc tccgagccga tgacctcgtc
    19021 gcccttcttg atcgagccga tgcggcggat gtccatgcgt accgaggcgt agaacttgag
    19081 cgcgttgccg ccggtggtgg tctcggggtt gccgaacatg acgccgatct tcatgcggat
    19141 ctggttgatg aagatgacga gcgtgttggt gcgcttgatg ttggcggtga gcttgcggag
    19201 cgcctggctc atgagccgcg cctgcaggcc catctgcggc tcgcccatct cgccctcgat
    19261 ctcggccttg ggcgtgagcg cggccaccga gtcgaccacg atcacgtcga ccgcgcccga
    19321 gcgcaccagc atgtcggcga tctcgagcgc ctgctcgccg gtgtcgggct gggagatgag
    19381 cagctcgtcg atgttcacgc cgagcttctt cgcgtactgc gggtcgagcg cgttctcggc
    19441 gtcgatgaag gcggcggtgc cgcccatctt ctgcatctcg gcgatcacct gcagcgtgag
    19501 cgtggtcttg cccgaggact cggggccgta gatctcgacc acccggccgc gggggaggcc
    19561 cccgacgccg agcgccaggt ccaggcccag cgacccggtg gacaccacct gcacgtcctt
    19621 gatggcctcg gagtccatgc gcatgatgga gcccttgccg tactgctttt cgatctgcga
    19681 cagcgccgcg gccagcgcct tggatttgtt ctcgtccatc gtgtacctct tgttgtgctt
    19741 gtcggcatct acggcgggag gacgatcccc cgggccggca ccggatactg tataaacgtc
    19801 catgtattct aatcgttgac cgaccgctgt caacgcagcg gtggtaaatt ccgctttcct
    19861 gaatcagaac aatctcttgg aggaagccat ggcgaaagtc gcttttctcg gcctgggcgt
    19921 gatgggctac ccgatggcgg ggcacctgaa gaaaaagggc ggccacgagg tgaccgtgta
    19981 caaccggtcg gccgccaagg cccagcagtg ggtgaaggag tacggcggcc agtccgcccc
    20041 caccccgaaa gaagcggccc agggctgcga cttcgtcatg atgtgcgtcg gcaacgacga
    20101 cgacgtgcgc gcggtcgcct acggcgacag cggggcgctc gccgggctga agaagggcgc
    20161 catcctggtc gaccacacca cggcctccgc cgtggtggcg cgcgagctgc acgccaagtg
    20221 caaggaaaag ggggtgggct tcgtcgatgc gcccgtctcg ggcggccagg caggcgcggt
    20281 gaacgggcag ctgggcatca tgtgcggcgg cgagcaggcc gacttcgaca aggcgaagcc
    20341 ggtgatcgac gtctacgcca agatggcggc gctgatcggc gcacccggcg ccgggcagct
    20401 caccaagatg gtgaaccagg tcgcgatcgt cggcctgctg cagggcctct cggaagcgct
    20461 caacttcggc aagaacgcgg gcctcgacat ggaaaaagtg gtggccgtca tctccaaggg
    20521 cgcggcgcag tcctggcagc tcgagaaccg ctggaagacg atggccgagg gcaagttcga
    20581 gggcttcggc ttcgccagcg actggatgag gaaggacctc aacatcgcgc tcgccgaggc
    20641 gaagaagaac ggttcctaca tgcccggcac cgcgctcatc gagcagtact tcggccgcat
    20701 cagcgccgcg ggacatgggc gctgggacaa caccgcgctg atttcgctgc tgaagaagag
    20761 ctaggcgcgc agccagcccc tgacgcgcgg atgcccgagc gccgccgcgt cgaccacgaa
    20821 acggggcgcg cgccccgcgt gcaccgcgag caccgcgccc acggcggtgc gcgcgatgtt
    20881 ggcaaagagc tcgtcggtcc agcacagcga atgcggcgtg acgatcacgt tgtcgagcct
    20941 caggatgggg ttgtcggccg gcgtgggctc ctcctcgaac acgtcgagcg cggcgccggc
    21001 gatcttcttc gcggcgagaa ctgcgtacag cgccttttcg tccaccaccg gcccgcgcgc
    21061 gacattgatc aggtaggcgc tcgacttcat gcgcgcgagc tgcctcgcgt tcaccaggtg
    21121 ccgcgtctct tcgttgagca aggccgtgac gacgacgaag tcggcttcgg ccatcaacgt
    21181 ctcgagcggc atcttcctcg cgccgatata gccgagctcg agctcgtcgg cgtagggatc
    21241 ggcggcgagc agcctcaggt cgaacgcctt cgccattcgc agcagctcct tgccgatgcg
    21301 tcccgccccc accacgccga gcgtgcggcc ggtgagtccc atgcccatgt gctcgacccg
    21361 gtcgttccag cggccttcgc gcgtcatgcg gtccttggcc atgagacggt gcgcgagcgc
    21421 cagcacgaga gtgagcgcca tggtggccac gggccgcggc atggcgttcg gggtgttgca
    21481 gaccagcacc cctgccttgc tcatcgctgc cacgtccacg ctgtcgtagc ccacgccgtg
    21541 gcgcgacacg actttcaggc gcaggtcctt gcccgccacc gcggccgccg gcaccttcgg
    21601 tgagttgacg tagagcgcgt cgtacttttc ggcgaagtcc gcggtgatct cgcggatgcc
    21661 cctgggcagc cattcccact cgagccccgt cgcggcatcg agcgcctcga gcggctcgcg
    21721 cccgaacacg ggctcgcccc tggcgtcgag gatgtcgctg gtgacgccga gcttgaagct
    21781 catgccttgc gcatcgcggc gaggctggcg cggatcgccg cctgcagcat gccctgatcg
    21841 gtacccgtgg caatcatgcg aaaaccgtag tccaggtact ccttgcccaa agccgcgtcc
    21901 gcggccatgt agccggcggc cttgccgtgc tttttcgccg cggcgacgat tctccggatc
    21961 gcgtcctgga ataccgggct gccgaactgg cccgggatac cgaggaagtt ggagaggtcg
    22021 aagtgcccga gccagagcgc gtccacgccc ggcaccgcgg cgatggcgtc cacgttctcc
    22081 aggcccgact tggtctcgat catggcgatg acgagcgtgc gctcgtggat cagcttcatc
    22141 ttgtcgacca cgtcgccgcc ttcgtagtcg tcgtgcgcga agccgaacgc ggcgccgcgc
    22201 cggccgcccg cgggcgggta gcgcgtgcac gagacgatga actgcgcctg ctcggcggta
    22261 tcgacgagcg gcaccatcac gccgagcgcg ccgcagtcga gggcgcgggc gatgaagtcg
    22321 tattcggtcc ccgggactct gaccataggc acgacgccga gcgcgcggca cagcgcgaac
    22381 tggggcttga gcgtctcgaa cgaggcgccg gtgtgctcca tgcagtacag ggcgtactcg
    22441 gcgcccgcgt tcttcagcag gcgcggcatg ccgggggtga agaactcgaa gatcatcgag
    22501 cccaaggcgg tgccgcccgc ggcgagcttg ttcttcacgg cgttttcgcg catgacgtgg
    22561 attatcgctg aagccgctgc agcttccagg tcgcgagcgg cccgagcagc ccgccaaggc
    22621 cgagcgtcgc ccaggccacg ccccaggcga atgcgtcgcc cgacatcttg ccgccgccgg
    22681 cgaggtcgag cgcccagccg aacaccaccg ggctcaccac gcccgtgccg aaccccacca
    22741 ccgagcgcac ggcgtacgcc gcgccgagga tgtgcggcgg cacgctctcg gcgatcaccg
    22801 tcgaatgcac cgacgagtcg gcgatgcccg caaagttgta caggcacgcg agcgccacca
    22861 gcagcgcgac cggcagcgcg atcagccagc cgatcgagaa cgacagcgcg agactgacgc
    22921 acgaccacag caggatggtc tgcgtgcggc cccagcggtc ggacatgatg ccgccggtga
    22981 tcgagccgcc gatgtttgcg acgtaggtga gcgcggagag cgacaggccg agcgccgcgg
    23041 cttgcgcgcc gctcgagccg ccggcgacca gcgccgcggt gaggaacgtc ggcatccacg
    23101 cccacaggcc gagcagctcc cagctgtgga aggtgtagcc ccacaccgag agcatgcccc
    23161 tgcggttctt gagcaccgcg gggatcgagg cgagcaccgt gtgcccctcg ggccgcgggt
    23221 gcacgacgtt gggcgtggcg cgtgtcgccg cgagcgcgat gagccaggac gcgagcggca
    23281 tcgccgccac cgcgccgagc gcggcgcgcc agccagcaac actcacgacg agccccgcga
    23341 cgccgagcgc aagcgcgtag cccgcgctcg aggcggcgat catcagcccc atggcgcgcc
    23401 cgcgcctgtt cgccgccacg tgctcgttca ccagcgccag catcggcgtg tagaacccgc
    23461 cctgggtgag cccggtgagc gcgtgcagcc acagcgccga ccaaaagccc tcgacgaaga
    23521 gcgcaaacgc gatcgggctc agcgcgccgg cgatcccggt gacgagaaag gcgcgcttgg
    23581 cgccgaagta gtcggcgatg aagccgacga gaaagagcga gctcaggtag ccgaggtgga
    23641 aggcgccctg gatggccccg gcgtcgcgcg ccgagaggcc ccagtcctcg cgcaccagcg
    23701 gcagcaccgc ggagtaggcg acaaaccagg ccgccgagag gacacggctc gcgcaaagca
    23761 gcgccagcca gcgccccgag cccgcgacgc tcgtcacgcc gccgcgagcc gaaccgggat
    23821 gcgccgcggg ccgaagggct tggtgaactt cctgtagcgc ccgggaagct gcgcgccgca
    23881 ggaaaggcac gcgccggctt ccgacagtcg ataccggttg atgcgatacc agtcgcgcac
    23941 gatcaccgcc tcgccgcagg acgggcagaa ggtcgtgccg ccttcggcgt cgtgcacgtt
    24001 gccggtgtac acgtagcgca gccccgcgcg cagcgcgatc tcccgcgccc gcgtcaaagt
    24061 cgccgtcggc gtcgccggaa tgtcgttcat cttccagtcg gggtgaaacg cggtgaagtg
    24121 cagcggcacg tcgggcccga ggttctcgta gatccacttc gattcggcct caagctcctc
    24181 gtccgaatcg ttcttgcctg ggatcagcag cgtggtgatc tcggtccaga cctcggtctg
    24241 cttcaccaga tacgccagcg tgtccagcac cggctgcagg tgcgcgccgc acagcttgaa
    24301 gtagaactcg tcggtgaacg ccttcaagtc gacgttcgcc gcatccatct tggcgtagaa
    24361 ctcccgccgc ggctcggcat ggatgtagcc cgccgtcacc gccacgttct tcacgccctg
    24421 ctcgcggcac gcgtcggcga tgtccatcgc gtactcggcg aagatcaccg ggtcgttgta
    24481 ggtgtaggcg acggacgcgc agcccatctg gaccgcggcc ttcgcaatgg cctcgggcgc
    24541 ggccgcatcc tgcagcctgt cgagctcgcg cgacttggag atgtcccagt tctggcagaa
    24601 cttgcaggcg aggttgcagc ccgcggtgcc gaacgagagc accgcggtgc ccgggtagaa
    24661 ctggttgagg ggcttcttct cgatcggatc gacgcagaag cccgaggagc ggccgtaggt
    24721 ggtgagcacc atgcggccgc cttccatcat tctcacgaag cacagcccgc gctggccctc
    24781 gtgcagccgg cagtcgcgcg ggcacaggtc gcactgcacg cgcccgtccg ccagcgcatg
    24841 ccaccagcgg ccggggtgcg acgtttctag tgcggcgcgg gctgcgtcca tttgatcacc
    24901 cgatagcgcc acagcttgca gcgcgcaagg cgcgtatcgg ccgggatgcc cgccttgcgc
    24961 atgagctcct cgaggaaccg gcgcgtctcg ggcaacccct cccatacctg cggcaggaag
    25021 gtgccccgct tgccctcgta ctccaggatc acgccgtcct cgcccgggcg cagctgcgcg
    25081 aggagctcgg cctcgtcggc gaagcgcagg ggcttgggcg gcgagagcac cgacacctcc
    25141 acctggcagc gcgaccattc ctcgcgcgtg agcggcgcaa agcgcgggtc gcgcagcgcc
    25201 gcgccgagcg cgttctccgc cacgtccaag ccgatgctgc gcgtcggcgc aaggctgccg
    25261 atgcagccgc gcagcttccc gtccagtctc agcgtgacga aggtggcgcc gggacgcgcc
    25321 agccacggcg cgacctcggt gcgcggcggc tcggcgtaca cgccgagcgc gtggccgatg
    25381 gcggcgcgcg cgatggcgag cagcgcgagg cccgcgtcct gcagcgtcac gcgctcgccc
    25441 tcgtacagcg caaatgcgga gtagcccacc acgcggtccc tgccgcccgc gctgtcgccc
    25501 gaattgcacg cgcccagcag gcggatcgac aagccgcgcg ccttggccgt ggcgagaaag
    25561 ccgttcagcg gcgtcgcccc gcaggcctcc tcgtggttga tgtcggtgtc gaacgcggcg
    25621 atgcgcgcga gcgtcgcctg gtcgatggcg cgtgcctcgt cgtaggggtg atagtgggaa
    25681 aggtcggtcg acaccacgaa cagcgtctcc ggcccgcccc agagccgctc gatcacgtcg
    25741 cgcacttcct gcggccgcgc gtcgccgacc gcgagcggca ccagcgcgaa cttgcccagc
    25801 attttctgca ggaagggcag ctgcacttcc agcgaatgct cggcggcatg cgccgccgcg
    25861 ctcgtcacca cctgcttgaa cggcgccagc atgcgcaggg cgtccgcgtc cagcggcacg
    25921 cgcccgagcg gcgtgtcgaa cgcctcgacg ccaggcaggg cgaggccgcg caccggcacg
    25981 cggtgcacgg ggccgagcag caccacgcgc ccgacgatgc cgcgcgccgc ggcaagctcg
    26041 tcgtaggcgc gcgccgccac cgcgccggaa tagatgtagc cggcgtgcgg cacgatgagc
    26101 gccttgggat ggccgaagcg cggcgcgagg ttctccacgc cgtcgatcag gtcggcgact
    26161 tcccgttgca gcgcgcgcgc gtcgcgcgga tagaacgtgc ccgccacggc ggcgggacgg
    26221 atctggctca cagcagctcc cgtacccttt cgagcgcgtg aacgacggct tggcggcgca
    26281 cgctgtcgcg atcgccgtca aagcgcctgg tctcactggt gatcttactc ccgtgcgccc
    26341 aggcgaagca caccaggccg acgggcttgt cgcgggtgcc gccgccaggc ccggcgaccc
    26401 cggtgacggc gagcgccacc tgcacccgtg cgcgccgcgc cgcccccgcc gccatttcgc
    26461 gcgcgacctg ctcgctgacc gcgccgtact ggcgcagcgt cgcctccgcg acgccgaggt
    26521 cctcgcgctt ggcgtcgttc gagtaggtga cgtagccgcg ctcgaaccag tccgagctcc
    26581 ccgggatcga ggtgagcgcc atcgccaccc agcccccggt gcaggactcg gcggtggcga
    26641 gccgctcgcc ccgcgccttc agcgcctcgc cgatgccgcg cgccaggtcg ttcatggaaa
    26701 cagcaacctc ttggcaagcg ccaggacgag cagcgtatag cccgcggcaa ggatgtcgtc
    26761 gaacatcacg ccaaagccgc ccttcatgcg cttttcgagc tggcggatcg gcggcggctt
    26821 cacgacgtcg aacaggcgaa acagcaggaa cgcggccgcc tgccagtgcc gctcctgcgg
    26881 cacgatcgcc agcaccagca ggaacgccac cacctcgtcc cagcacatgc cgccgtggtc
    26941 ggcgatgccg agatgccgcc cggtgatgtc gcaggcccag acgccggcca aaaacagaaa
    27001 cgcgaggatc gccagcagca cgagcggcgg gtacgccggg ccgatcagcc accagatcgg
    27061 caaggcgatc agcgtgccca cggtgccggg cgcgaaaggc gacagtccgg cgccgaaacc
    27121 gaaggcgatg aaatgcgccg ggtgcgtgaa cacgaagcgc gcgctcgggc ggaagaccat
    27181 catgcgaaat gatcgaatcc ggccgcgact tcgatctcgc ggccttgggg gtcgaggagc
    27241 cgcaggcgcg gcgcgccggc gtggatcgac cccacgcgcg tcagcgcgac gccgagcggc
    27301 gcggcgagct gcccgatcgc cgcgcgcgcc gccgcgggcg cggtgaacgc gagctcgtag
    27361 tcgtcgccgc cggagagcgc gcagcgcgct tccagggctg cgtcgccgat gcggaacttg
    27421 ggcagatcgg cgtagcgcac cgccgcaccc acgccggagc gctcgaggat gtggcccaga
    27481 tcctgcgcaa agccgtcgga gacgtcgatc gccgcgcttg ccagcccgcg cagccgctcg
    27541 cccagggcca cgcgcggctc gggcaggtgc aggcgcgcgg cggccttcgc gttctccgga
    27601 tgctcgagcg cgaacgcggc gccgccgagc tcgcccgaca cccagacgtc gtcgccgggc
    27661 ttcgcgccgt cgcggcgcag cgccgcgccc gccggcacat cgccgaggat ggtgacgcag
    27721 atggaaagcg ggccgcgcgt cgtgtcgccg ccgaccaggt ccaccttgaa gcgcgtggca
    27781 agcgcataga agccttcggc gaacgcggcg agccagtcct cgtcggccct gggcagcgcg
    27841 agcgcgagcg tcgcccagcg cgggctggca cccattgccg ccatgtcgga aaggttcacc
    27901 gccagcgcct tgtgcccgag cgcgcgcggc tcggcgcgcg cggcgaagtg gcgcccttcg
    27961 aggagcagat ccgtggacac cgcgagctcg cagccggccg ccggcgccag gagcgcggcg
    28021 tcatcgccca cgccgagcgt ggcgttgcgc gccgggcgcg agaagtagcg gcggatgatc
    28081 tcgaactcgg acggcatcgc cgtcctcagg cgggctggcg cccgcccgcg gtttcctgcg
    28141 cgcgcagccc gcgcgcgagc ttctccagca cgccgttcac gtacttgtgg ccgcccgcgc
    28201 cgccgaagct cttgcccagc tcgatggcct cgttgatcac caccttgaac ggcgtttccg
    28261 ggtgcgcctg cagctcgaac gcgccgatgt agaggatggc gcgctccacg ggcgagaggc
    28321 gccggaactc gcggtcgagg cacggcgcga tcaggccctc gagctcggcg gcgcgcgcct
    28381 gcacgccgcg caccagcgcc tcggcgaggt cgcggtcggt gcgcccgtcg tcgtcgatgg
    28441 cgcgcacctg ctcgagcgca tcgccgctgc ccatctgcca ggcatagagc gcctgcagcg
    28501 cgatctcgcg cgagcgccgg cgcgggccct tcgctgcctt catttcggcc acgccgccac
    28561 cttggcgcgc aggcgcgcca tctcgagcgc cacgcgcacc gcttccgcgc ccttgtccag
    28621 gcgcgcctcg gcctgcttct cgtcgtcggt ggtgaggatg ccgttggcga ccggaatgcc
    28681 gaattccagc gctacgtcca tgacgccgcg cgccgactcg ttcgccacca cctcgaagtg
    28741 gtaggtgtcg ccgcgcacca cgcagccgat ggcggccagg cagtcgaact tgccgctctg
    28801 cgccagccac tgcaacgcga gcgggatctc gagcgcgccg ggcacctctt ccacggtaag
    28861 ctcgtgcacc ccgagcttct tcgcctcgtc gcgcgcgcgc tgcaggagct tgccgctgag
    28921 cgcgtcgttg aagcggctcg ccacgatgcc caggcgcatg gcgctagccc cgcggcgact
    28981 cgacgtagcc ggtgacctcc aggccgaagc ccgtcatgct cggcatcttg cgcggcgcgg
    29041 ccagcaggcg catgcggccc acgcccagct cgcgcaggat ctgcgcgccg atgccgtagg
    29101 tgcgcaggtc ggcgctgcgg ccggtacgcg cgggccgggt ttcggcaagg cgcgcgtcga
    29161 tggcgtccga ggcgaggttg cagttgagca gcaccaccac gccggtgccc gccttggcga
    29221 tcgcggtgag cgcctcgccc acgccccagg tgtgcgtgcc gggaccggca tcgagcaggt
    29281 cgacgaccga gagcggctcg tgcacgcgca ccagggtctc ggcctcgcgc gcgggcgtgc
    29341 ccaccaccag cgcgagatgc gtgccgcgcg tcggcacgtc gtgaaacgtc accaggtgaa
    29401 agcgccccca cgcggtgtcg atgtcgcgcg cgagcccgcg cgtgacgagc gactcgtggc
    29461 ggctgcggta ctcgatgagg tcggcgatgg tgccgatgcg caggccgtgc tcggcgcaga
    29521 actcgagcag gtcgggcagg cgcgccatgg tgccgtcgtc cttcatcacc tcgcacagca
    29581 ccgccgcggg cgccagcccg gcgagccgag caaggtcgca gcaggcctcg gtatggccgg
    29641 cgcgcaccag cacgccgccc gcctgggcga cgagcgggaa cacgtggccc ggctgcacga
    29701 tgtcctcggc cctggcctcg ggcgccgcgg ccaccttgat ggtgagcgcg cgatcggctg
    29761 ccgagatgcc ggtggcgatg c
//
LOCUS       DBNHKE010000054         3950 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_220312, whole genome shotgun sequence.
ACCESSION   DBNHKE010000054 DBNHKE010000000
VERSION     DBNHKE010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_220312"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16O9_03440"
     CDS             complement(<1..225)
                     /locus_tag="AC16O9_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515703.1"
                     /translation="MPPVQTPMITNTPPALVVPAARDATEARGERREPQLLQLVPADE
                     RERAARAEGALEAKAEKSDFQELIERSTGQL"
     gene            complement(438..1322)
                     /locus_tag="AC16O9_03445"
     CDS             complement(438..1322)
                     /locus_tag="AC16O9_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6515704.1"
                     /translation="MSVRRFRSGELQLAYRLVNGGRPKLRGLRGPALQRPVPRLPAAL
                     PLVFVHGMAAFSYDWVPVASALGREAACLDMRGFGDSDWSGDYSVPAMAGDIGALLDH
                     LGWARAIVVGHSMGARIACYYASRHPRRVAGLALVDYAPEYAAEGLRRAAERLVAVPE
                     VFASVEAAMAHFGAPREARVRFAAYLLKVEGGYAVKRDTWFRDQARKVLAGTAPRPRV
                     DMWKALARLRAPTLVVRGTRSELFAPESAAKMQAANALIRVAEVQAGHNVAGENITGL
                     VGVLRGFVAGVDRGAAGA"
     gene            complement(1597..2856)
                     /gene="fahA"
                     /locus_tag="AC16O9_03450"
     CDS             complement(1597..2856)
                     /gene="fahA"
                     /locus_tag="AC16O9_03450"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HIR6515705.1"
                     /translation="MQTRRLRSWVESANDPATDFPIQNLPFGVFRRKGANEAPRGGVA
                     IGDQILDLAAVGLRTGPNLNKLAAAGRPVWRQLRRVLQLALSDKSKAKRFSKYLVPMN
                     KAELFLPVQIGDYSDFYAGIHHALAVGRMLRPDAPLLPNYKWVPIGYHGRASSVVVSG
                     TPIVRPNGQTKAPEAAAPSFGPSRRLDYEVELGIVIGPGNPLGKPVGIRRALDHVFGV
                     VLLNDWSARDIQAWEYQPLGPFLAKSFATTVSPWIVTLEALEPFRCPAFPRVGEDPRP
                     LPHLTDEADQQQGGYAIGTDMTLSSAQMRAKKLAPARLSRCSFRDAYWTIAQIVAHQS
                     SNGCNLQPGDLLGSGTLSGTEPGSAGSMLELTQGGKTPLQLPGGETRAFLADGDEVIQ
                     RGRCEAQGAVPIGFGEAAGVVKPTPKG"
     gene            complement(2907..>3950)
                     /gene="hmgA"
                     /locus_tag="AC16O9_03455"
     CDS             complement(2907..>3950)
                     /gene="hmgA"
                     /locus_tag="AC16O9_03455"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR6515706.1"
                     /translation="GLLRSGPFDEVEASPNRLRWDPLPVPAKPTDFIDGLFTIAGSGD
                     AAAQSGIAVHVYRANRPMKNRYFYSADGELMFVPQQGAILLATELGRLEVAPGEVAVV
                     PRGTKFRLEGNGAVRGYLCENYGAPFRLPELGPIGSQGLAQKRDFLAPVAAYEDKAAK
                     CQIVAKFHGGLWGAACSHSPLDVVAWHGNYTPYKYDLARFMAINTVSFDHPDPSIFTV
                     LTSPSGQPGVANCDFVIFPPRWMVAEHTFRPPWFHRNVMSELMGLVRGVYDAKAEGFL
                     PGGVSIHNCMSAHGPDVASYDKASKAVLKPHKIEDTLAFMWESRYVFRPTKAALAAKE
                     LQKNYDRVWDGFK"
BASE COUNT          595 a         1323 c         1423 g          609 t
ORIGIN      
        1 gagctgcccc gtggagcgct cgatcaattc ctggaagtcg ctcttctcgg cctttgcctc
       61 gagtgcgcct tccgcgcgcg ccgcgcgctc gcgctcgtcg gccggcacga gctgaaggag
      121 ctgcggctcg cgccgctcgc cgcgcgcttc ggttgcgtcg cgcgctgcgg gcaccaccag
      181 cgccggcggc gtgttggtga tcatcggcgt ctgcaccggc ggcaccgtga tctgcggcgc
      241 ctggggcgcg gcataaccgc ccgtttgcgc gtgggcggcc ggaagaacga acgcaagagc
      301 gaaaagaagg gcgcggcgca tcgtcggtac ccgtagagct gggatgccga ggattctagc
      361 cctctcagtg agaatcaggg ggcgggcccc tgaaagcaaa ggcaaaacct ggttctgcct
      421 cccctgttcg aggggggtca ggcgcccgcg gcccctcgat ccacccccgc cacgaacccc
      481 cgcagcaccc ccaccagccc ggtgatgttc tcccccgcga cgttgtgccc agcttgcacc
      541 tccgcgaccc ggatgagcgc attggccgcc tgcatcttcg ccgccgactc gggagcaaag
      601 agctccgagc gcgtgccgcg cacgacgagc gtgggcgcgc gcagccgcgc gagcgccttc
      661 cacatgtcca cgcgcggccg cggcgcggtc ccggcgagca ccttgcgcgc ctggtcgcga
      721 aaccaggtgt cgcgcttcac ggcgtagccg ccctcgactt tcagcaggta cgcggcgaag
      781 cgcacgcgcg cttcacgcgg cgcgccgaag tgcgccatgg ccgcttcgac cgacgcaaag
      841 acttccggca cggcgacgag gcgctcggcc gcgcggcgca ggccctcggc cgcgtactcg
      901 ggcgcgtagt cgacgagcgc gagcccggcg acgcgccgcg ggtggcgcga ggcgtagtag
      961 caggcgatgc gcgcgcccat cgagtggccg acgacgatgg cgcgcgccca gcccaggtga
     1021 tcgagcagcg cgccgatgtc gccggccatc gccggcaccg agtagtcgcc cgaccagtcc
     1081 gagtcgccga agccgcgcat gtcgaggcac gccgcctcgc gcccgagggc ggaggcgacc
     1141 ggcacccagt cgtacgagaa cgcggccatg ccgtgcacga agacgagggg cagggcggcg
     1201 gggagcctgg ggactggcct ctgcagggcc gggccgcgaa ggccgcgcag cttcggcctg
     1261 ccgccgttca ccagccggta ggcgagctgc agctcgcccg agcggaagcg ccgcacgctc
     1321 acggcacgga cggatcgagc gcgggcaggt ccctcggccg ggcggagatc ggggcggagc
     1381 cgggcgcgtg cccgtccgcc gcgcgggtgg accggtcccg ctctaaggtg tagtcgcctc
     1441 tcaggttggc ggttgcgttg gtactggcgt tcatcggagg cactgcctta tcgggttctc
     1501 tatggtctcg ccttttctgc cgggagaatc atccggcaga aaaggcgaga tgtataaggg
     1561 ccaagaccgc gataaatagg gacagacctc atttatttac cccttcggcg tcggcttcac
     1621 cacccccgct gcctcgccga agccgatcgg caccgcgcct tgggcctcgc agcggccgcg
     1681 ctggatcact tcgtcgccgt cggcgaggaa ggcgcgggtc tcgccgccgg ggagctggag
     1741 cggggtcttg ccgccctggg tcagctcgag catcgagccc gcgctgccgg gctcggtgcc
     1801 cgagagggtg cccgagccga ggaggtcgcc cggctgcagg ttgcagccgt tggagctctg
     1861 gtgcgcgacg atctgcgcga tcgtccaata ggcgtcgcgg aagctgcatc tggacaggcg
     1921 cgcaggcgcg agcttcttcg cgcgcatctg cgcgctgctc agggtcatgt cggtgccgat
     1981 ggcatagccg ccttgttgct ggtcggcttc gtccgtgagg tggggcagcg gccgcgggtc
     2041 ttcgcctacg cgcgggaagg ccgggcagcg gaagggctcg agcgcttcca gggtcacgat
     2101 ccagggcgag acggtggtgg cgaagctctt ggcgaggaag gggccgagcg gctggtactc
     2161 ccaggcctgg atgtcgcgcg ccgaccagtc gttcagcagc accacgccga acacgtgatc
     2221 gagcgcgcgc cggatgccga cgggcttgcc gagcgggttg ccggggccga tgacgatgcc
     2281 gagctcgacc tcgtagtcga ggcgccgcga cgggccgaag gagggcgccg cggcctccgg
     2341 ggccttggtc tggccgttgg ggcgcacgat cggcgtgccc gaaactacta ctgaagaggc
     2401 gcggccgtgg tagccgatcg gcacccactt gtagttcggt agcagcggcg cgtcgggccg
     2461 caacatcctg cccaccgcga gcgcgtggtg gatgccggcg tagaagtcgc tgtagtcgcc
     2521 gatctgcacg ggcaggaaga gctcggcctt gttcatcggc acgaggtatt tcgagaagcg
     2581 cttcgccttc gacttgtcgg aaagcgcgag ctgcagcacg cggcgcagtt gccgccacac
     2641 cgggcggccg gcggcggcga gcttgttcag gtttggccca gtcctgaggc cgaccgcggc
     2701 gaggtcgagg atctggtcgc cgatcgccac gccgccgcgc ggcgcctcgt tcgcgccctt
     2761 tcgccggaac acgccgaacg gcaggttctg gatcggaaag tcggtcgccg gatcgttcgc
     2821 cgactcgacc cagctcctca gcctacgcgt ttgcataaat ggggacagac ctcattattc
     2881 cgctatgttg aaaatgaggt ctgtccctat ttaaagccgt cccacacgcg gtcatagttc
     2941 ttctgcagct ctttcgcagc gagcgcggcc ttggtggggc ggaagacgta gcgcgactcc
     3001 cacatgaacg cgagggtgtc ctcgatcttg tgcggcttca gcaccgcctt ggaggccttg
     3061 tcgtaggacg cgacatccgg cccgtgcgcg ctcatgcagt tgtggatcga cacgccgccc
     3121 ggcaggaacc cctcggcctt cgcgtcgtac acgccgcgca ccagccccat cagctcgctc
     3181 atcacgttgc ggtggaacca gggcgggcgg aaggtgtgct cggcgaccat ccagcgcggc
     3241 gggaagatga cgaagtcgca gttggcgacg cccggctggc ccgagggcga agtaagcacg
     3301 gtgaagatcg acggatccgg gtggtcgaag ctcacggtgt tgatcgccat gaagcgcgcg
     3361 aggtcgtact tgtacggagt gtagtttccg tgccatgcca ccacgtcgag cggcgagtgg
     3421 ctgcacgcag cgccccacaa cccgccatgg aacttggcca cgatctgaca cttggccgcc
     3481 ttgtcttcgt aggccgcgac tggggcgagg aagtcgcgct tctgcgcgag gccctgcgag
     3541 ccgatggggc cgagctcggg tagccggaag ggcgcgccgt agttctcgca caggtagccg
     3601 cgcaccgcgc cgttcccttc gaggcggaac ttggtgccgc ggggtacgac agcgacctcg
     3661 ccgggcgcga cctcgagtcg gccgagttcg gtggcgagca ggatcgcgcc ctgttgcggc
     3721 acgaacatca gctcgccatc ggcgctgtag aaataacggt tcttcatcgg gcggttggcg
     3781 cggtacacgt gcaccgcgat gcccgattgc gcggcggcgt cgcccgagcc ggcgatggtg
     3841 aagagtccgt caataaagtc ggtcggcttg gccgggacgg gcagcgggtc ccaacgcagg
     3901 cggttcggcg aggcttccac ctcgtcgaac ggcccggagc gcagcagccc
//
LOCUS       DBNHKE010000055         2869 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_855001, whole genome shotgun sequence.
ACCESSION   DBNHKE010000055 DBNHKE010000000
VERSION     DBNHKE010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_855001"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="AC16O9_03460"
     CDS             <1..1605
                     /locus_tag="AC16O9_03460"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HIR6515707.1"
                     /translation="AEAAGVLFRLHGAPMYFYRRGRGRYHAAPKETLERALAGLEKKK
                     RIQAAAAEWTAALIRFECPAEILALRDELLYAPDRNKPETRAFEDACKATGLVPARLF
                     ERCGVLDDSHDFHFNRFLHEFFPRGAGFPEHELPNVPQDLPLAPVEAVSLDDAGTTEV
                     DDAFSVERLADGMLRIGVHISAPALSIAGGSPIDAIARDRLSTAYMPGHKITMLPPDV
                     IAQYSLEAGTTRPAVSLYLTVSPEDWSIRGAHSRIEQVKIAGNLHHGAYAGLNEAFPE
                     DRSLGLPHEEDLRVLWRFALALEAARGKASVNAALPDYSFALEGERVIITTRRRGAPL
                     DKMVSELMILANARWGELLAERDVAAIYRAQTSGKVRLSVHAEPHEGLGVSCYAWMTS
                     PLRRYVDLVNQRQLVASLSGRRAPLGRTSEGLLSAMRAFELTYARYDEHQRAMETYWS
                     LRWLLQESAFEQEGVVAREGVVRIVRLPLSLRVPSLPDLLPGTRVRVVLGDIDLLERT
                     VVCTYRETLAETVAETGLEGDEAPAT"
     gene            1845..2666
                     /locus_tag="AC16O9_03465"
     CDS             1845..2666
                     /locus_tag="AC16O9_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR6515708.1"
                     /translation="MLLHGIALSVHFKFPDAIIFKSPDTHLEVVLVNARTKEKPSKAE
                     LLAQANLDRGGNTDKAEIARSALPVTNPREPGRDLAEAKRRVEQLEAQQRKLLAQAKQ
                     ATPASVPVETAAPKLAPSEEAPQTPSGRELADLTMAALKLQAAIDRQTHEYQKRPRKK
                     FIGARATEYRFAQYEEDWRLKVERVGTLNYPPEARGKLYGNLRLTVTLRPDGSVDAIE
                     LDRSSGLKVLDEAAFRIVRMASPYGAFPPEIRRDTDLLVITRTWFFGDGDQVWTK"
     gene            2698..>2869
                     /gene="aroE"
                     /locus_tag="AC16O9_03470"
     CDS             2698..>2869
                     /gene="aroE"
                     /locus_tag="AC16O9_03470"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059698.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6515709.1"
                     /translation="MSDRYAVIGNPVAHSRSPGIHAAFARATGQAMTYERIEAPLDGF
                     ARTVAAFRTAGAR"
BASE COUNT          452 a         1029 c          989 g          399 t
ORIGIN      
        1 gccgaggcgg ccggcgtgct cttcaggctg cacggcgcgc cgatgtactt ctaccggcgc
       61 ggccgcggcc gctaccacgc ggcaccgaag gagacgctcg agcgcgcgct ggccgggctg
      121 gagaagaaaa agcgcatcca ggccgcggcg gccgagtgga cggcagcgct gatccgcttc
      181 gaatgccccg cggagatcct cgcgctgcgc gacgaactgc tctacgcgcc ggaccgcaac
      241 aagcccgaga cccgcgcctt cgaggacgcc tgcaaggcca ccggactcgt cccggcgcgc
      301 ctgttcgagc gttgcggcgt gctcgacgac agccacgact ttcacttcaa ccgcttcctg
      361 cacgagttct tcccgcgcgg cgccgggttt ccggagcatg aactgccgaa cgtgccgcag
      421 gacctgccgc tcgccccggt ggaggcggtc agcctggacg acgccggcac caccgaggtc
      481 gacgacgcct tctcggtgga gcggctggcc gacggcatgc tgcgcatcgg cgtgcacatc
      541 tcggcgccgg cgctgagcat cgccggcggc tcgccgatcg acgccatcgc ccgcgaccgg
      601 ctctcgaccg cgtacatgcc cgggcacaag atcaccatgc tgccgccgga cgtgatcgcg
      661 cagtattcac tggaagccgg caccaccagg ccggcggtgt cgctctacct gacggtgtcg
      721 ccggaggact ggtcgatccg cggcgcgcac tcgcgcatcg agcaggtaaa gatcgcgggc
      781 aacctgcacc atggcgccta cgccgggctg aacgaggcct ttcccgagga ccgcagcctg
      841 ggcctgccgc acgaggaaga cctgcgcgtg ctgtggcgct tcgcgctggc gctcgaagcc
      901 gcgcgcggca aggcgagcgt gaacgccgcg ctgccggact attccttcgc gctggagggc
      961 gagcgcgtga tcatcaccac gcgccggcgc ggcgcgccgc tcgacaagat ggtctcggaa
     1021 ctgatgatcc ttgcaaacgc gcgctggggc gaactgctgg ccgagcgcga cgtggccgcg
     1081 atctaccgcg cgcagacctc gggcaaggtg cgcctgagcg tgcatgccga gccgcacgaa
     1141 ggccttggcg tgtcctgcta cgcgtggatg acctcgccgc tgcgacgcta cgtcgatctc
     1201 gtcaaccagc gccagttggt ggcctcgctg tcgggccggc gcgcgccgct cggccggacc
     1261 tcggaaggcc tgctcagcgc gatgcgcgcc ttcgagctca cctacgcccg ctacgacgag
     1321 caccagcgcg ccatggaaac ctactggagc ctgcgctggc tgctgcagga atcggccttc
     1381 gagcaggagg gtgtcgtggc gcgcgaaggg gtggtgcgca tcgtgcgcct gccgctctcg
     1441 ctgcgcgtgc cctcgctgcc cgacctgctg cccggcaccc gggtgcgggt ggtgctcggg
     1501 gacattgacc tgctggagcg aaccgtggtc tgcacctacc gggaaacgct cgcggaaacg
     1561 gttgcggaaa cgggtctgga gggcgacgaa gcgcctgcca cctagcgaac gaccggaacg
     1621 atggggcttc gccccctcgt gcacccccga cgtcagcgga actcgtcgaa tgactgcgcc
     1681 gcaaggcgcg ttgccaccgg gttccgtggc gtagaatgcg gcaggctctg gaccgcgagg
     1741 tgaatttgct gggaagcgtt gcgacaggcg cgtggcgccc gccgtggcgg tgggcaaaat
     1801 tcctcaacgg aatcggcgtc ctgcacgttt ccgtcggcct ctcgatgctg ttgcacggca
     1861 tcgccctgtc ggtgcacttc aagttcccgg acgcgatcat cttcaagtcg ccggacaccc
     1921 acctggaagt cgtgctggtg aacgcgcgca ccaaggaaaa gccctcgaag gcggaactgc
     1981 tcgcgcaggc caacctcgat cgcggcggca acaccgacaa ggccgagatc gcccgttccg
     2041 ccctgccggt gacgaacccg cgcgagcccg ggcgcgacct cgccgaggcg aagcggcgcg
     2101 tcgagcagct cgaggcgcag cagcgcaagc tgctcgcgca ggcgaagcag gcgacgccgg
     2161 cgagcgtccc ggtcgagacc gcggcaccga agttggcgcc gagcgaggaa gcgccgcaga
     2221 cgccctcggg ccgcgagctt gccgacctga ccatggcggc gctcaagctg caggcggcga
     2281 tcgaccgcca gacccacgag taccagaagc gcccgcgcaa gaagttcatc ggcgcgcgcg
     2341 ccaccgagta ccgcttcgcc cagtacgagg aggactggcg gctcaaggtc gagcgcgtcg
     2401 gcacgctcaa ctacccgccc gaggcgcgcg gcaagctcta cggcaacctg cggctcaccg
     2461 tcacgctgcg gcccgacggc agcgtcgacg cgatcgaact cgaccgctcc tccgggctga
     2521 aggtgctgga cgaggcggcg ttccgcatcg tgcgcatggc ctcaccctac ggcgcctttc
     2581 cgccggagat ccgccgcgac accgacctgc tggtgatcac gcgcacctgg ttcttcggcg
     2641 acggcgacca ggtctggacc aagtagccat cgccacgccc gtgtcgccgg ggtgcgcatg
     2701 agcgatcgct acgcggtgat cggcaatccg gtggcgcatt cgcgctcgcc cgggatccac
     2761 gcggccttcg cgcgcgccac cgggcaagcc atgacctatg agcgcatcga ggcgccgctg
     2821 gacggcttcg cccgcaccgt ggccgcgttt cgcaccgccg gcgcccgcg
//
LOCUS       DBNHKE010000056        12123 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1014372, whole genome shotgun sequence.
ACCESSION   DBNHKE010000056 DBNHKE010000000
VERSION     DBNHKE010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1014372"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="AC16O9_03475"
     CDS             <1..193
                     /locus_tag="AC16O9_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR6515710.1"
                     /translation="GWAVLRPGACPCCVGRAQLPVELARLIRHERPPGVQIELADPAH
                     EPALRRALGERPLADYVAL"
     gene            complement(190..510)
                     /locus_tag="AC16O9_03480"
     CDS             complement(190..510)
                     /locus_tag="AC16O9_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR6515711.1"
                     /translation="MLGAFGAHALKARLPAEMLALWSTGVQYHLWHALGMILIGLAAA
                     VVADGMWLRAAGLLLLVGIVLFSGSLYALALGAPKWLGAVAPLGGAAFILGWLALALA
                     AARA"
     gene            complement(561..662)
                     /locus_tag="AC16O9_03485"
     CDS             complement(561..662)
                     /locus_tag="AC16O9_03485"
                     /inference="COORDINATES: protein motif:HMM:NF028380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HIR6515712.1"
                     /translation="MGKGDKRTRRGKIYKGSYGKTRPHREKQAKKKS"
     gene            complement(699..4415)
                     /gene="metH"
                     /locus_tag="AC16O9_03490"
     CDS             complement(699..4415)
                     /gene="metH"
                     /locus_tag="AC16O9_03490"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR6515713.1"
                     /translation="MTHRDRTAQLQALLAERILVLDGAMGTMIQRERLDETQFRGARF
                     HDWPRDLRGNNDLLVLTQPELIRGIHSQYLEAGADIIETNTFNSTAISMADYGMESLV
                     PELNLAAAKLAVDAARAAEERDPSRPRFVAGVLGPTTKTASISPDVNDPGFRAVTFDQ
                     LVAAYAEAAAALIEGGVDLILVETIFDTLNAKAALFALDELFERRGTRLPVIVSGTIT
                     DASGRTLSGQTAEAFYNSIRHARPLAVGLNCALGAKELRPYVEEIAGIAEAFVSCHPN
                     AGLPNAFGEYDDTPENMAAIMGEWARSGWLNIVGGCCGTTAEHIRALADAVRGVTPRK
                     VPALPPQLRLSGLEPLNVGAESLFVNIGERTNVTGSKAFARLVLAGDYAEALSVARQQ
                     VENGAQVIDVNMDEAMLDSEAAMHRFLTLVAAEPDIARVPVMIDSSKWPVIEAGLKCM
                     QGKCIVNSISMKEGEAEFLRQARLARRYGAAVVVMAFDEQGQADTLERRTAICRRAYE
                     LLTTQAGMPAEDIIFDPNIFAIGTGLEEHARYGLDYIEAARWIRQNLPHAQVSGGLSN
                     LSFSFRGNDAVREAMHTAFLYHAVRAGMSMGIVNAGQLGVYEEIEPQLRERVEDVILN
                     RRDDATERLIEFAENVKQGGKAAKEDDAWRRAPVRERLVHALVTGTATHVVADAEEAR
                     LEAAHPIEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLVPFIEAEKA
                     KSGEGGRPKGKIVIATVKGDVHDIGKNIVAVVLQCNNYEVLNLGVMVPAQKILETARV
                     EKADAIGLSGLITPSLEEMAHVAREMEREGFTLPLLIGGATTSRAHTAVKIAPNYSGP
                     VVYVPDASRSVPVAQNLLSKEARDAYIAEVRADYEKVRRQHAAKKGPGPLHSIARARE
                     LGYAADWKSYAPPAPAKPGLTVLANYPLEKLAPYIDWGPFFQTWELSGPYPKILEDPV
                     VGAEARKLFADAQAFLKKMIDGRWVRASGVCGLFAAARVGDDVEIYRDATRRDLAMTW
                     HNLRQQNQKPAGNPNQCLADYVAPKDSGVPDWVGAFAVTAGLGMERKLAEFERSHDDY
                     SAIMLKSLADRLAEAFAEHLHERVRREYWGYAGNEALDNVALVAEKYRGIRPAPGYPA
                     CPDHTEKAALFALLDASGNAGMTLTESFAMLPAAAVSGFYLSHPAASYFAVGRIGRDQ
                     LEDYARRKGMRVEEAERWLAPYLAYDPAQQVQAA"
     regulatory      complement(4459..4535)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            4591..4986
                     /locus_tag="AC16O9_03495"
     CDS             4591..4986
                     /locus_tag="AC16O9_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6515714.1"
                     /translation="MDHLEPKRAFEFLKQHADALFVDCRSEMEYLFVGHPVGALHVAW
                     NDGPDWAVNPHFVGQVKKLAGTNHTTRPVVLICRSGNRSQEAGEELERAGFTRVYNVR
                     HGFEGELNDNHQRNSLAGWRYEGLPWEQC"
     gene            4992..5420
                     /locus_tag="AC16O9_03500"
     CDS             4992..5420
                     /locus_tag="AC16O9_03500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6515715.1"
                     /translation="MQFRSITATFACQVHWGDCDPAGIIFYPTYFRWMDAACWAMFAG
                     IGYDAKRMRAEHLAMPLLSADCQFLYPAAQGDHCEVRSKVEQFGGKSFTVAHEVLRAD
                     GVALARGAEKRVWARFDDGPGTRLRGVAVPEELIAALRAD"
     gene            complement(5423..5986)
                     /gene="pcaG"
                     /locus_tag="AC16O9_03505"
     CDS             complement(5423..5986)
                     /gene="pcaG"
                     /locus_tag="AC16O9_03505"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HIR6515716.1"
                     /translation="MKLVASGSQTVGPYLHIGLTWLNTERIAGPGVKGERVVVQGRLL
                     DGDGAGVSDGLVEIWQANAAGRYPHPDDRRKLPLEKGFRGFGRIPTDAKGRFRFSTVK
                     PGRVPGPGGKLQAPHLNVTVFMRGLLKQLSTRIYFPGEAANADDPLLKRVPAARRATL
                     VAKRKARGMLEWNIVLQGRNETVFFDF"
     gene            complement(5986..6690)
                     /gene="pcaH"
                     /locus_tag="AC16O9_03510"
     CDS             complement(5986..6690)
                     /gene="pcaH"
                     /locus_tag="AC16O9_03510"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HIR6515717.1"
                     /translation="MSKLYGYRRYQPRTQPKYDFPFYASTAKRHPTKPLVIVPQTLSE
                     VTGPVFGHDDVKPTDHDLTRQHAGEPIGERIIVSGRVLDESGRPVPHALVEVWQANAA
                     GRYPHKADQHDAPLDPNFSGAGRTVTDAQGHYRFITIRPGEYPWRNHYNAWRPAHIHF
                     SLFGRAFLTRLVTQMYFPGDPLLTLDPMYMSIPDEKARRRLVSQFDLQNTVPEHALAY
                     RFDIVLRGRAATPMER"
     gene            complement(6693..7328)
                     /gene="maiA"
                     /locus_tag="AC16O9_03515"
     CDS             complement(6693..7328)
                     /gene="maiA"
                     /locus_tag="AC16O9_03515"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HIR6515718.1"
                     /translation="MKLYTYFRSSAAFRVRIALHLKGLAYEPAFVHLAKGEHLQPAYA
                     KINPQGLLPALQVDGTLLAQSIAIMEYLEETHAQPPLLPADPLARARVRSLSLIVACE
                     IHPLNNPRVLKYVKNALGHSQDEVNAWYRHWIADGLAKLEAELARPGTGRHSHGDTPT
                     MADCCLVPQIFNAQRFECDLKPYPTLMRVFDACMQLDAFDRAQPSKQPDAE"
     gene            complement(7325..8017)
                     /locus_tag="AC16O9_03520"
     CDS             complement(7325..8017)
                     /locus_tag="AC16O9_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6515719.1"
                     /translation="MAHVIPLWSPPALPVAGGGDAFPVRRIFCVGRNYAEHQKEMGGS
                     GREQPFFFIKSAHALVPGGGDVHYPPKTSNYHYETELVVALGKGGRRIPAGRANEHVF
                     GYAVGLDMTRRDLQQWGKDHGRPWDFGKDFDEAAPCSPLVPASQIGHPAKGAIWLQVN
                     GKQRQNADLADMIWSVPEQIAYLSEYYTLESGDIIYSGTPAGVGPVKPGDELHAHIDG
                     VGELKVKMLPAL"
     gene            complement(8046..9563)
                     /locus_tag="AC16O9_03525"
     CDS             complement(8046..9563)
                     /locus_tag="AC16O9_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIR6515720.1"
                     /translation="MNAADQLLGDDALARHGARTALICAGRRLSYAELAADMARAANA
                     LRALGVRPGERVLLLLRDTPEFAAAWLGAVHAGAVAVGLNSKLAEADYRHIVAESRAR
                     LLIADDELLPVLGAFGAELGSTQCLVRGAEWRAQTARAAPLARAHAARPGDAAFWLFS
                     SGTTGRPKGIVHGHPSVLVAGQAQREVLGLGPGDTVFATSKLFFAYALETGMLGPLAL
                     GATSILHPDWTDVEQICAIVARHKPTAVFSVPTLYRRLLALPPAQLAPFREVRHCVSA
                     GERMPQPVFESWRAATGREILSIYGMSETFCVIMMTPIGSAAALRSGKPLSGVQVRLL
                     ADDGREAANGEPGVLWVRHPALSSGYANRPEATREQFQDGWFCTRDLFVRDSHGFYSH
                     QGRSDELLKVAGQWVQPGELEEAVIGLPAVAEAACVAAPDEDGFERLALFVAARGGDP
                     RAALTAAAAACDEKLPRHKRPKWILPVAELPRTATGKVQRFKLRELLAQERRRKA"
     gene            complement(9694..10986)
                     /locus_tag="AC16O9_03530"
     CDS             complement(9694..10986)
                     /locus_tag="AC16O9_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6515721.1"
                     /translation="MKKLLSWTMPVLLLAHAGAALAQDKPALSAGDTAWMLISTVLVI
                     IMIVPGLALFYAGMVRAKNALSVLMHVFVTFSLISVLWALFGYSLAFTEGNAVIGGFS
                     KAFLAGVTPASLSGTIPEYVFVAFQLTFAAITPALVVGAFAERMKFSAVLWFLGLWLF
                     VCYAPMAHMVWGGGWIGGMGAIDFAGGTVVHINAGVAGLVAAIVLGKRKGLGKVAMPP
                     HNLTFTLTGAALLWAGWFGFNAGSAVAANGSAALAMINTQLATAAAVLGWMFAEWLIK
                     GKPSLLGAASGAIAGLVAITPACGNAGPMGAIVLGVVAGVGSFWAVSSLKRALGYDDS
                     LDVFGVHGVAGIIGALGIGILASPSFGGTGLGGENASIGAQLAVQALSVGFTLVYTGV
                     LSYILLKIIDMVIGLRVADEAETQGLDLAEHGEAAYNN"
     gene            complement(10997..11335)
                     /gene="glnK"
                     /locus_tag="AC16O9_03535"
     CDS             complement(10997..11335)
                     /gene="glnK"
                     /locus_tag="AC16O9_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6515722.1"
                     /translation="MKLISAIIKPFKLDEVREALSGIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIDVAVKTDLLERAIEAIEKAANTGKIGDGKIFVFELEKVIRI
                     RTGETDAEAL"
     gene            complement(11363..>12123)
                     /locus_tag="AC16O9_03540"
     CDS             complement(11363..>12123)
                     /locus_tag="AC16O9_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR6515723.1"
                     /translation="PSATLLEIDIMRKTILAAALAAAAALPGLASAQGSSPHSVSGNM
                     GVFSDYRFRGVSQTFGQPAIQGGIDYAHASGFYLGNWNSNVSAISYTDGSIEMDFYGG
                     YKFAAGPVGLDVGLLQYYYPGADSAGVKYDTLEAYVGASWKWFSVKYSVTLDDWFGVA
                     NSDGSGYLDIGFSYEIAPKLTLSAHYGDQTVKNSSASDYSDTKIGITYDLNGWALGLA
                     YVDTDIAASTTTTANGNSEDINKGTVVFSVSKSF"
BASE COUNT         1885 a         4112 c         4130 g         1996 t
ORIGIN      
        1 gggctgggcc gtgctgcggc cgggcgcgtg tccgtgctgc gtgggccgcg cacagctgcc
       61 ggtggagctg gcgcgcctga tccggcacga gcgcccgccg ggggtgcaga tcgagctggc
      121 ggatccggct catgagcccg cgttacgccg cgcgctcggc gagcggccgc tcgcggacta
      181 cgtcgcgctc taggcgcgcg ccgcggcgag ggcgagcgcc agccagccaa gaatgaacgc
      241 ggcgccgccg agcggcgcga cggcgccgag ccatttcggc gcgccgagcg ccagggcgta
      301 gaggctgccc gagaaaagca cgattccgac aagcagcagt aaccctgccg cgcgcagcca
      361 catgccgtcg gcgacgacgg ccgcggcaag gccgatcagg atcatgccga gcgcgtgcca
      421 caggtggtac tgcaccccgg tactccagag cgccagcatc tccgccggca ggcgcgcctt
      481 cagcgcgtgc gcgccgaatg cccccagcac gacgccgacg gcggcgagca gcgcgcccag
      541 cgcaatgcag agcttggggc tcaggacttc ttcttcgcct gcttctcgcg gtgcgggcgc
      601 gtcttgccgt agctgccctt gtagatcttg ccgcgccgcg ttctcttgtc gcccttgccc
      661 atgtcacctc tccactgttg gaccgcgccg cattatagct acgcggcctg cacctgctgc
      721 gccggatcgt aggcgaggta cggcgcgagc cagcgctcgg cctcctcgac gcgcatgccc
      781 ttgcgccgcg cgtaatcctc gagctggtcg cggccgatcc ggccgaccgc gaagtagctc
      841 gctgcgggat gcgagaggta aaaacccgac actgcggctg ccggcagcat ggcgaaggat
      901 tcggtgagcg tcatgccggc gttgccggac gcatcgagca gcgcgaacag cgccgccttt
      961 tcggtgtgat cggggcatgc gggatagcct ggcgcggggc ggatgccgcg gtacttctcg
     1021 gcaaccagcg ccacgttgtc gagcgcttcg ttcccggcgt agccccagta ctcgcgccgc
     1081 acgcgctcgt gcaggtgctc ggcgaaggcc tcggccaggc ggtcggcgag cgacttgagc
     1141 atgatcgcgg agtaatcgtc gtgcgatcgc tcgaactcgg cgagctttct ctccatgccg
     1201 agtcccgccg tgacggcgaa ggcgccgacc cagtcgggta cgcccgaatc cttcggcgcc
     1261 acatagtcgg ccaggcactg attcggattg ccggcgggtt tctggttctg ctgccgcagg
     1321 ttgtgccaag tcatcgccag gtcgcggcgc gtggcgtcgc ggtagatctc gacatcgtcg
     1381 ccgacccgcg cggcggcaaa caggccgcag accccgctgg cgcgcaccca gcgcccgtcg
     1441 atcattttct tcaggaaggc ctgtgcgtcg gcgaagagct tgcgcgcctc ggcgccgacg
     1501 accgggtctt cgaggatctt cgggtacggt cccgagagct cccaggtttg gaagaacggg
     1561 ccccagtcga tgtacggcgc gagcttctcc agaggatagt tggcgagcac ggtgagcccg
     1621 ggcttggcgg gcgcgggcgg cgcgtaggac ttccagtccg cggcgtagcc gagctccctc
     1681 gcgcgcgcga tcgagtgcaa cggccccggc cccttcttcg cagcgtgctg gcggcgcact
     1741 ttctcgtagt ccgcgcgcac ctccgcgatg taggcgtcgc gtgcttcctt cgacagcagg
     1801 ttctgcgcca ccggcaccga gcgcgaggcg tcgggcacgt agaccaccgg gcccgagtaa
     1861 ttgggggcga tcttcaccgc cgtgtgtgcg cgcgacgtgg tcgccccgcc gatcagcagc
     1921 ggcagcgtga agccctcgcg ctccatctcg cgggccacgt gcgccatctc ttcgagcgag
     1981 ggcgtgatca gcccggaaag tccgatcgcg tcggccttct ccacgcgcgc cgtctccagg
     2041 atcttctgcg ccggcaccat gacgccgagg ttgagcacct cgtagttgtt gcattgcagt
     2101 accacggcga cgatgttctt gccgatgtcg tgaacgtcgc ccttcaccgt ggcgatgacg
     2161 atcttgccct tgggacgccc gccctcgccg cttttcgcct tttccgcctc gatgaacggc
     2221 accaggtgcg cgaccgcctg tttcatgacg cgcgccgact tcaccacctg cggcaggaac
     2281 atcttgccgg cgccgaacag gtcgcccacc acgttcatgc cgtccatcag cggcccttcg
     2341 atcacctcga tggggtgcgc cgcctcgagc cgcgcttctt cggcgtcggc caccacgtgc
     2401 gtcgccgttc ccgtcaccag cgcgtgcacc aggcgctcgc gcaccggcgc gcgccgccag
     2461 gcgtcgtcct ccttcgccgc cttgccgccc tgcttgacgt tctccgcgaa ctcgatcagc
     2521 cgctccgtgg cgtcgtcgcg ccggttgagg atcacgtcct cgacccgttc gcgcaactgc
     2581 ggctcgatct cctcgtagac cccgagttgc cccgcgttga cgatgcccat cgacatcccg
     2641 gcgcgcaccg cgtggtacag gaatgcggta tgcatcgcct cgcgcaccgc gtcgttgccg
     2701 cggaacgaaa acgacagatt ggacagtcca cccgacacct gcgcgtgcgg caggttctgc
     2761 cggatccagc gcgccgcctc gatgtagtcc aggccgtagc gcgcgtgctc ttcgagcccg
     2821 gtgccgatgg cgaagatatt cgggtcgaag atgatgtcct cggcgggcat gccagcctgt
     2881 gttgtcagca gctcgtaggc gcggcggcag atggcagtgc gccgctccag cgtgtcggcc
     2941 tggccctgct cgtcgaaggc catcaccacc actgcggcgc cgtagcggcg cgcaaggcgc
     3001 gcctggcgca ggaattccgc ctcgccctcc ttcatgctga tcgagttgac gatgcatttg
     3061 ccctgcatgc acttcaggcc ggcctcgatc acgggccatt tggagctgtc gatcatcacc
     3121 ggcacgcgcg cgatgtcggg ctccgcggcg acgagcgtca ggaagcggtg catcgcagcc
     3181 tccgaatcca gcatcgcctc gtccatgttc acgtcgatga cctgggcgcc gttttctacc
     3241 tgctgacgcg caacgctgag cgcctcggca tagtcgccgg cgaggacgag acgcgcgaac
     3301 gccttcgagc cggtgacgtt ggtgcgctcg ccgatgttga cgaagagcga ttccgcgccc
     3361 acgttgagcg gctcgaggcc cgagaggcgc agctgtggcg gcaacgccgg taccttgcgc
     3421 ggcgtcacac cgcgaacggc gtcggccagc gcgcgaatgt gctcggcggt cgtgccgcag
     3481 cagccgccga cgatgttgag ccagccgctt cgcgcccact cgcccatgat cgcggccatg
     3541 ttctctggcg tgtcgtcgta ctcaccgaag gcgttcggca ggcccgcgtt cggatggcag
     3601 gaaacgaagg cctcggcgat gccggcgatc tcctccacgt aggggcgcag ttccttcgcc
     3661 cccagcgcgc agttgaggcc gacggcaagc ggccgggcgt ggcggatcga gttgtagaac
     3721 gcctcggcgg tctgccccga gagcgtgcgg cccgaggcgt cggtgatggt gcccgagaca
     3781 atcaccggca ggcgcgtgcc gcgccgctcg aacagctcgt cgagcgcgaa cagcgccgcc
     3841 ttggcgttca acgtgtcgaa gatggtctcg accaggatca ggtccacgcc gccttcgatg
     3901 agcgccgccg ccgcctcggc gtaggccgcg accagctggt cgaacgtcac ggcgcgaaag
     3961 cccggatcgt tcacgtccgg cgagatcgac gccgtcttgg tggtcggccc gagcacgccg
     4021 gcgacaaagc gcgggcgcga cggatcgcgc tcctccgcgg cacgggcggc gtcgaccgcg
     4081 agcttcgcgg cggccaagtt cagctccggc accaggctct ccatgccgta gtcggccatc
     4141 gagatcgcgg tggaattgaa cgtgttggtc tcgatgatgt ccgcgcccgc ctcgagatac
     4201 tggctatgga taccgcggat cagctccggc tgcgtcagca ccagcaggtc gttgttgccg
     4261 cgcaggtcgc gcggccagtc gtgaaagcgc gcgccgcgga actgcgtttc gtccaggcgc
     4321 tcgcgctgga tcatggtgcc catggcgccg tcgagcacga ggatcctctc ggcgagcagg
     4381 gcttgcagtt gagcggtacg gtcgcgatgc gtcataaggg cccgaaaagc aaaacgcccg
     4441 ttcggcttgc gcggaaacgg gcgcttcggc acgtctcttt agcagtattt gttacgcgcc
     4501 cgcaagctga acatcaaatc ggcgcgaagg gtgcagtgtg cctacttagt actgctgtgt
     4561 caaacgctta cgctataatg cgctccctcc atggaccatc tggaacccaa gcgggcgttc
     4621 gaatttctca agcagcatgc cgacgcgctg tttgtcgact gccgcagcga gatggagtat
     4681 ctcttcgtcg gccatcccgt gggcgcgctg cacgtggcgt ggaacgatgg gccggactgg
     4741 gccgtgaacc cgcatttcgt cggccaggtg aagaagcttg ccggcaccaa ccacaccacg
     4801 cgcccggtgg tgctgatctg ccgcagcggc aaccgctcgc aggaagccgg cgaggagctc
     4861 gagcgcgcag gcttcacgag agtctacaac gtgcggcatg gattcgaagg cgagctgaac
     4921 gacaatcacc agcgcaactc gctcgccggc tggcgctacg aagggttgcc ctgggaacag
     4981 tgctgagcgg ggtgcagttt cgctcgatca ccgcgacctt cgcctgccag gtgcactggg
     5041 gcgactgcga tccggcgggc atcatcttct atcccaccta cttccgctgg atggatgccg
     5101 cgtgctgggc gatgttcgcg ggcatcggct acgacgcaaa gcgcatgcgc gccgagcatc
     5161 tggcgatgcc gctcctgtcg gccgactgcc agttcctcta tcccgccgcg cagggcgacc
     5221 actgcgaagt gcgctcgaag gtcgagcagt tcggcggcaa gtccttcacc gtggcgcacg
     5281 aggtgctgcg cgccgacggc gtggcgcttg cgcgcggcgc ggagaagcgt gtctgggcgc
     5341 gtttcgacga cggccccggc acgcgcctac gcggcgtggc ggtgcccgag gagctgatag
     5401 cggcgctgcg cgccgactag cgtcagaagt cgaagaacac cgtctcgttc ctgccctgca
     5461 ggacgatgtt ccactcgagc atcccgcgcg ccttgcgctt cgcgacgagc gtcgcgcgcc
     5521 gcgctgcggg cacgcgcttg agcagcggat cgtcggcgtt ggccgcctcg ccgggaaagt
     5581 agatccgcgt cgagagctgc ttcagcagcc cgcgcatgaa cacggtgacg ttcaagtgcg
     5641 gtgcctgcag cttgccgccc ggccccggca cccggccggg cttcacggtg ctgaagcgaa
     5701 atcgaccctt ggcgtcggtc gggatgcgcc cgaagccgcg gaatcccttc tccagcggca
     5761 gcttgcgccg gtcgtccggg tgcggatagc ggcccgcggc gttcgcctgc cagatttcga
     5821 ccagcccgtc ggacaccccg gcgccgtcgc cgtcgagaag gcgcccctgg acgacgacgc
     5881 gctcgccctt cacgccgggg cccgcgatac gttcggtgtt gagccacgtc agcccaatgt
     5941 gcagatacgg cccgacggtc tgcgagccgc tcgcgacgag cttcatcaac gctccattgg
     6001 cgtcgccgcg cgcccgcgca ggacgatgtc gaagcggtag gcgagcgcgt gctcgggcac
     6061 cgtgttttgc aggtcgaact gcgataccag gcgccggcgc gctttctcgt ccgggatgct
     6121 catgtacatg gggtcgagcg tcagcagcgg gtcgccggga aagtacatct gcgtcaccag
     6181 acgcgtgagg aacgcgcgcc caaagagcga gaaatggatg tgcgccgggc gccacgcgtt
     6241 gtagtggttg cgccacgggt actcgccggg ccggatggtg atgaagcggt aatgaccttg
     6301 ggcatcggtg accgtgcgcc ccgcgccgga aaagttcggg tcgagcggcg cgtcgtgctg
     6361 gtcggccttg tgcgggtagc gcccggcggc gttcgcctgc cacacctcga ccagcgcatg
     6421 cggcaccggc cggccgcttt cgtccagcac gcggccgctg acgatgatgc gctcgccgat
     6481 cggttcgccc gcgtgctggc gcgtgaggtc gtggtccgtc ggcttgacgt cgtcgtggcc
     6541 gaacacgggg cccgtgacct ccgacagcgt ctgcggcacg atgacgagcg gcttggtcgg
     6601 gtggcgcttg gcggtcgagg cgtagaaggg gaagtcgtat ttcggctggg tgcggggttg
     6661 gtatcgccga tacccgtaga gcttggacat cttcactccg cgtcgggttg cttcgacggc
     6721 tgtgcgcgat cgaaggcatc gagctgcatg caggcgtcaa acacgcgcat gagcgtcggg
     6781 taaggcttca ggtcgcactc aaagcgctgc gcgttgaaga tctgcggcac caggcagcag
     6841 tccgccatcg tcggcgtgtc gccatggctg tgccggcccg tgccgggccg cgccagctcg
     6901 gcttccagtt tggcgagccc gtcggcgatc cagtgccggt accaggcgtt gacctcgtcc
     6961 tgactatggc cgagggcgtt cttgacgtac ttcagcacgc gcggattatt gagcgggtgg
     7021 atctcgcagg caacgatcag cgagaggctc ctcacgcgcg cgcgcgccag gggatcagcg
     7081 ggcagcagcg gcggctgcgc gtgggtttct tccagatact ccatgatcgc gatcgactgc
     7141 gcgagcagcg tgccgtcgac ctgcagcgcc ggcagcaggc cctggggatt gatcttggcg
     7201 taggccggct gcaggtgctc gcccttggcg aggtgcacga aagccggctc gtaggcgagc
     7261 cccttcaggt gcagcgcgat gcggacgcgg aaagccgccg agctgcggaa gtacgtgtac
     7321 agcttcacag cgccggcagc atcttcacct tgagttcgcc cacgccgtcg atgtgcgcgt
     7381 gcagctcgtc gccgggcttc accgggccga cgcccgcggg cgtgcccgaa tagatgatgt
     7441 cgccggactc gagcgtgtag tactcggaca ggtaagcgat ctgctcgggc accgaccaga
     7501 tcatgtcggc gaggtccgcg ttctggcgct gcttgccgtt cacctgcagc cagattgcgc
     7561 ccttggccgg atggccgatc tgggatgccg gcaccagggg actgcacggc gcggcctcgt
     7621 cgaaatcctt gccgaagtcc cacggccggc cgtggtcctt gccccactgc tgcaggtcgc
     7681 gccgcgtcat gtccagcccc acggcatagc cgaacacgtg ctcgttggcg cgtccggccg
     7741 ggatgcgacg tccgcccttg ccgagcgcca ccactagctc cgtttcgtag tggtagttcg
     7801 aggtcttggg agggtaatgc acgtcgccgc cccccggcac cagcgcgtgt gcgctcttga
     7861 tgaagaagaa cggctgctcg cggccgctgc cgcccatttc cttctgatgc tcggcgtagt
     7921 tgcggccgac gcagaagatg cggcgcaccg ggaaggcatc gccgccaccg gcgactggca
     7981 gcgcgggcgg cgaccacagc ggaatgacat gagccatgcg gacctcgtcg gggaaaacgc
     8041 cgattttatg ctttgcggcg gcgctcttgc gcgagcagtt cgcgcagctt gaagcgctgc
     8101 accttgccgg tggcggtgcg cggcagctcg gcgaccggca ggatccactt tgggcgcttg
     8161 tggcgcggca gcttctcatc gcaggcggcg gcagcggccg tcagcgccgc gcgtgggtcg
     8221 ccgccgcggg cggcaacgaa cagcgccagt cgctcgaagc cgtcttcgtc cggtgccgcc
     8281 acgcacgcgg cctcagcgac ggcgggcagg ccgatgaccg cctcctcgag ctcgcccggc
     8341 tgcacccact ggccggcgac cttcagcagc tcgtcgctgc gcccttgatg cgaatagaag
     8401 ccgtggctgt cgcgcacgaa gaggtcgcgg gtgcagaacc agccatcttg gaactgctca
     8461 cgcgtcgctt ccggccggtt ggcgtagccc gagctaagcg ccgggtggcg cacccagagc
     8521 accccgggct cgccattcgc ggcctcgcgc ccgtcatcgg ccagcaggcg cacctggacg
     8581 cccgagagcg gcttgccgct tctcagggcc gccgcgctgc caatcggcgt catcatgatg
     8641 acgcagaaag tctcggacat gccgtagatg gagaggatct cccgcccggt cgccgcgcgc
     8701 cagctctcga acaccggctg cggcatgcgc tcgcctgcgg acacgcagtg gcggacctcg
     8761 cgaaacggcg ccagttgcgc tggcggcagc gccagcagcc ggcgataaag cgtcggcacg
     8821 ctgaacacgg ccgtgggctt gtggcgcgcg acgatggcgc agatctgctc gacgtcggtc
     8881 cagtcggggt gcaggatcga agtcgcgccg agggccagcg gccccagcat gccggtttcc
     8941 agcgcgtagg cgaaaaagag cttcgaagtc gcgaacacgg tatcgcctgg ccccagaccc
     9001 agcacctcgc gctgcgcctg gcccgccacc agcacgctgg ggtgcccatg cacgatcccc
     9061 ttggggcggc cggtggttcc ggaggagaac agccagaacg cggcgtcccc cgggcgcgcc
     9121 gcgtgcgccc gcgcgagcgg cgcggcgcgg gccgtctgcg cacgccactc ggcgccgcgt
     9181 accaggcact gggtgctccc gagttccgcg ccgaacgccc ccagcaccgg aagcaactcg
     9241 tcgtcggcga tcaagagccg cgcccggctc tcggcaacga tgtggcggta gtccgcctcg
     9301 gcgagcttgc tgttcaggcc cactgccacg gcgccggcat gcaccgcgcc gagccaggcg
     9361 gcagcgaatt ccggcgtatc gcgcagcagc agcagcacgc gctcgcccgg ccgcacgccc
     9421 aatgcgcgca gcgcattggc cgcgcgcgcc atgtccgcgg cgagctcggc gtagctcagc
     9481 cggcgcccag cgcagatgag cgccgtgcgc gcgccatggc gcgcgagtgc gtcatcgccc
     9541 aggagctggt cggcggcgtt catcgcgatt cccggcgcag tataggcggg caggcggcgc
     9601 ggacaaaaaa atcggggcgc ttgcgcgccc cgtgaactag ggttgagccc cggagtaggc
     9661 tgttttaccg ctgcaagcgg aaatcagttg ttatcagttg ttgtaggccg cctcgccatg
     9721 ctcggcgagg tccagaccct gtgtttccgc ctcgtcggcc acccgcaggc cgatcaccat
     9781 atcgatgatc ttgagcagga tgtagctcag cacgcccgta tagacgaggg tgaatcccac
     9841 gctcagcgcc tgcacggcga gttgcgcgcc gatgctggcg ttctcgccgc ccaagccagt
     9901 gccgccgaag ctcggcgagg cgaggatacc gatgccgagc gcgccgatga tgccggcgac
     9961 gccgtgcacg ccgaacacgt cgagcgagtc gtcgtagccc agcgcgcgct tcagggacga
    10021 cacggcccag aacgaaccca cgccggcgac gacgccgagg acgatggcgc ccatcgggcc
    10081 cgcgttgccg caggccggcg tgatcgccac caggccggcg atcgcgccgg aggcggcgcc
    10141 gagcaggctc ggcttgccct tgatgagcca ttcggcaaac atccagccga gcacggcggc
    10201 cgccgtggcg agctgcgtgt tgatcatggc cagcgccgcg ctgccgttgg ccgccacggc
    10261 gctgccggcg ttgaacccga accagcccgc ccacagcagt gcggccccgg tgagcgtgaa
    10321 ggtcaggttg tgcggcggca tcgccacctt gcccaggcct ttgcgcttgc cgagcacgat
    10381 cgcggcgacc aggcccgcaa cgcccgcatt gatgtgcacc accgtgccgc ctgcgaagtc
    10441 gatcgcgccc atgccgccga tccagccgcc gccccagacc atgtgcgcca ttggcgcgta
    10501 gcacacgaac agccacagac cgaggaacca gagcactgcg gagaacttca tgcgctcggc
    10561 gaacgcgccg acgacgagcg ccggcgtgat ggcggcgaag gtgagctgga acgcgacgaa
    10621 tacgtactcg ggaatggtgc ccgagagcga tgctggcgtg acaccggcga ggaacgcctt
    10681 ggagaatccg ccgatcacgg cgttgccctc ggtgaacgcc aggctgtagc cgaacagcgc
    10741 ccacagcacc gagatcagcg agaaggtgac gaatacgtgc atcagcaccg agagcgcgtt
    10801 cttcgcgcgc accatgccgg cgtaaaagag cgccaggccc ggcacgatca tgatgatgac
    10861 cagaaccgtc gagatcagca tccaggcggt atcgccggcg ctgagggcgg gcttgtcctg
    10921 cgccagcgcc gccccggcgt gggcgagcag caggaccggc atggtccatg aaagcagctt
    10981 cttcatgtgc gtctccctag agcgcttccg catcggtttc gccggtgcgg atgcggatga
    11041 ctttttcgag ttcgaacacg aagatcttgc cgtcgccgat cttgccggtg ttcgcggcct
    11101 tctcgatcgc ctcgatggcg cgctcgagca ggtcggtctt caccgcgacg tcgatcttca
    11161 ctttcggcag gaaatcgacc acgtactccg cgccgcggta gagctcggtg tggcccttct
    11221 ggcggccgaa gcccttcacc tcggtcaccg tgatgccctg cacgccgatg cccgagaggg
    11281 cttcgcgcac ctcgtcgagc ttgaacggct tgatgatggc tgaaatcagt ttcattcctg
    11341 tctcccgaag acggcccgag tgttagaacg acttgctgac ggagaagacc accgtgccct
    11401 tgttaatgtc ttcgctgttc ccgtttgccg tcgtggtcgt ggatgcggcg atatccgtgt
    11461 ccacgtaggc aagccccagc gcccatccat tgaggtcgta ggtgataccg atctttgtgt
    11521 cggagtagtc ggacgcggaa ctgttcttga cggtctggtc gccgtagtgc gcggagagcg
    11581 tcagcttcgg cgcgatctcg tagctgaagc caatgtcgag atagcccgag ccatccgagt
    11641 tcgcaacgcc gaaccagtcg tccagcgtca ccgagtactt cacgctgaac cacttccagc
    11701 tcgcgccgac ataggcttcc agggtgtcgt acttgacccc tgcggagtca gcgccggggt
    11761 agtagtactg cagaaggccg acgtcgaggc cgacggggcc agcggcgaac ttatacccgc
    11821 cgtagaaatc catttcgatc gagccgtcgg tatagctaat ggcgctgacg ttggagttcc
    11881 agttgccgag gtagaagccg ctcgcgtgcg cgtagtcgat gccaccctgg atcgcgggct
    11941 ggccgaacgt ctgcgatacc ccgcggaaac ggtagtcgct gaagacgccc atgttcccgg
    12001 acaccgagtg cggcgaggag ccctgcgcgc tcgcaaggcc cggcagggcc gcagcggcgg
    12061 caagtgcagc ggcgaggatg gtcttgcgca tgatgtcgat ctccaaaagg gttgcggatg
    12121 gca
//
LOCUS       DBNHKE010000057        22696 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1437731, whole genome shotgun sequence.
ACCESSION   DBNHKE010000057 DBNHKE010000000
VERSION     DBNHKE010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 22696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22696
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1437731"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..162
                     /locus_tag="AC16O9_03545"
     CDS             61..162
                     /locus_tag="AC16O9_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515724.1"
                     /translation="MRAIAELYIGARYGSEPARGAELARRVRELRLA"
     gene            159..1172
                     /gene="mltB"
                     /locus_tag="AC16O9_03550"
     CDS             159..1172
                     /gene="mltB"
                     /locus_tag="AC16O9_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIR6515725.1"
                     /translation="MSARRLLLALLVAALAATARAEEDETAAPYAERTDVQGFIRELV
                     ARHDFNAEELALVFAGARREEAVLTAMRAQPRQADSWDAYRALFVNERHLRAGLEFWR
                     AHRPVLARARRAYGVPEEIIVAIIGIETFYGRNTGRWRVIDALATLAFDYPPRAPFFR
                     GELENYLLFARELGLDVFAVKGSYAGAIGIPQFMPGSYLRYAVDFDGDGATDLRGNPA
                     DAIGSVANFLNKHGWQRGAPVQLGARVTGDGHEPLVAGGPLPRHTLAELADAGVEARG
                     APLPAETRAVLLRLETPERPADYRLGLQNFYVLTRYNRSVFYALSVADLAEQLRAARR
                     KAR"
     gene            complement(1180..2160)
                     /locus_tag="AC16O9_03555"
     CDS             complement(1180..2160)
                     /locus_tag="AC16O9_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6515726.1"
                     /translation="MTTFASRCSASLLAAALAALGGCASEPVQAPVTDIRPPPALKPA
                     PEPEPPPPAVAAGVPPGHYMIKRGDTLYSIALEHGADYRDVARWNQLDDPTKISVGQV
                     LRVTPPAGETSVQVGAARGQAPLQARPLDGAGTAQQGRGDGSTKVAPKALRLPYSEEN
                     LALLSRGDAAAVAPTLKPVAVAPSAAPAKPEPPTAAVDRDPESLDFIWPARGGLLAKF
                     SEPSSKGVDIGGKPGDPVLAAAAGQVLYTGTGIRGFGKLIVIRHDNGFSSVYAHNREI
                     LVKEGQNVARGQRIAEMGDSDADRPKLHFEIRKSGKPVDPLRYLPGERPS"
     gene            complement(2157..2843)
                     /locus_tag="AC16O9_03560"
     CDS             complement(2157..2843)
                     /locus_tag="AC16O9_03560"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6515727.1"
                     /translation="MKAHGPRDAAGTGMTSQRTRLRMVERLREKGIRDERVLAAMGAV
                     PRHLFVDEALASRAYEDTALPIGFSQTISQPYVVARMIESLIGDNGVPARVLEVGTGC
                     GYQAAVLAQLAREVYSVERVRALLERARANLRGLRLSNLRLTHGDGNLGLQEAAPFDA
                     IIVAAAARAVPQVLLQQLAPGGRMVLPLKPPQAPHGTQRLALLERKARGISETTLDEV
                     LFVPLETGKL"
     gene            complement(2840..3583)
                     /gene="surE"
                     /locus_tag="AC16O9_03565"
     CDS             complement(2840..3583)
                     /gene="surE"
                     /locus_tag="AC16O9_03565"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6515728.1"
                     /translation="MRILVSNDDGYFSPGIAQLAERLGALGEVTVFAPERDRSGASNS
                     LTLDRPLSVRRAPNGFFFVNGTPTDCVHLAVTGVLDFLPDIVVSGVNLGANMGDDTIY
                     SGTVAAATEGFLLGIPSLAVSLASKEGRFIATAVDVAQRLVERFGRAPLGQPVLLNVN
                     VPDVAPEALGAVEVTRLGRRHKAEPVVKMRTPRGEQAYWIGAAGEAADAGPGTDFHAV
                     ASGRVSVTPLQVDLTHPAQLAPVRAWLAA"
     gene            complement(3587..4753)
                     /locus_tag="AC16O9_03570"
     CDS             complement(3587..4753)
                     /locus_tag="AC16O9_03570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6515729.1"
                     /translation="MGSAVADTAHAELREGVRALCAQFDSAYWQKVDEARAYPEAFVD
                     ALTGAGWMAALIPEQYGGSGLSLTEATVIMEEISRSGGNPGCCHGQMYNMNTLLRNGS
                     EAQKRKYLPKIASGELRMQAMGVTEPTAGTDTTKIKTVAVKKGDRYVVNGQKVWTSRL
                     QHSDLMILLARTTPLAEVNKKSDGMSVFLVEKKAALGKGLTMRPIRNMVNHETNEVFF
                     DNLEIPAENLIGEEGKGFRYILDGLNAERILIAAECIGDGYWFVGRATNYAKDRVVFD
                     RPIGQNQGIQFPIAEAYMEVEAANLMRYKAAALFDAHQHCGAEANMAKHLAAKASWEA
                     ANACLQTHGGFGFAAEYDVERKFRESRLYLVAPISTNLILSYVAEHVLGLPRSF"
     gene            complement(4738..5643)
                     /locus_tag="AC16O9_03575"
     CDS             complement(4738..5643)
                     /locus_tag="AC16O9_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515730.1"
                     /translation="MVKDKIVLVTGAGGGIGRDFALAFAAHGAKVVVNDLGTSVAGEG
                     ASAGPAQKVVDEIKARGGEAIASADSVADWESANRLVKASIDAYGHLDCVVNNAGILR
                     DRFFFNMSVEEWRSVIDVHLNGSFYVSRAAAPYFKSQNSGCYVHMTSTSGLIGNLGQA
                     NYAAAKLGIVGLSKSIALDMARYGVRSNCIAPFAWSRMIGSIPTETPEQKARVEKMKV
                     METAKIAPLAVYLASDAAHDVSGQVFAVRANELFLMSQSRPVRSVHRAEGWTPQEIAA
                     HGMPALRQSFFPLDRTQDVFTWDPL"
     gene            5938..6132
                     /locus_tag="AC16O9_03580"
     CDS             5938..6132
                     /locus_tag="AC16O9_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515731.1"
                     /translation="MQLKEPDSAAFGAEALPGGLREINAQRRGEPLAPVSAPAPTQPA
                     SLVGLIALAKRYFGWGTTRR"
     gene            complement(6244..6320)
                     /locus_tag="AC16O9_03585"
     tRNA            complement(6244..6320)
                     /locus_tag="AC16O9_03585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6284..6286),aa:Pro,seq:ggg)
     gene            complement(6336..6722)
                     /locus_tag="AC16O9_03590"
     CDS             complement(6336..6722)
                     /locus_tag="AC16O9_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6515732.1"
                     /translation="MESRNHSKKADLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEF
                     TQLKPVKRRGNRRYYQHHEVLLIRRIRELLYEEGFTISGARNRLDNAAHGADERSEPA
                     PSAAAAGGDLAAIRRELRALLEALRA"
     gene            complement(6691..7008)
                     /locus_tag="AC16O9_03595"
     CDS             complement(6691..7008)
                     /locus_tag="AC16O9_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR6515733.1"
                     /translation="MTLTKAELADLLFEKVGLNKREAKDMVEAFFEEIRGALERGESV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPISARRVVTFHASQKLKAMVEKARHGKPQPQ
                     QES"
     gene            complement(7031..9412)
                     /gene="pheT"
                     /locus_tag="AC16O9_03600"
     CDS             complement(7031..9412)
                     /gene="pheT"
                     /locus_tag="AC16O9_03600"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6515734.1"
                     /translation="MNVPERWLRSFCDPSMKADKLAQLLTMSGLEVEACVPVAPPFSG
                     VVVGEILQVERHPNADRLTVCTVSTGQQKLAVVCGAPNARPGMKAPLATVGTRLGSLE
                     IKAAELRGVQSQGMLCSARELGLSDDHSGLLELPVTAKAGQDVRKALDLDERVFTFKL
                     TPNRADCLSILGIAREVAALTGAKLTAPRPKPVAARSKAKHPFGVTHAAGCGRFAGRV
                     IRGVNAAAPTPHWMRQRLERAGQRSISALVDVTNYVMLELGRPLHVYDQDKLRGPIDV
                     RWGRKGEKVLLLNGQEVEVDADVLCITDDSGPIGLGGIMGGESTKAETTTRNLFLESA
                     FFFPDAVAGRARRFNFTSDAAHRFERGVDFGNNVAGIERATQLILEICGGEPGPTVDL
                     VKRLPKRKPVRMRVARAQKVIGMPVAEKDMARAFKRLGLPSRRQRGAFTVAPPSFRFD
                     LEIEEDLIEEVARVHGFERIPALPPLAPARMAATPEARRPVHALRERLAGRDYQEVIT
                     FSFVEPQWEADLAGASNPIRLLNPIASQLSVMRSTLLGGLLANVRYNAARKLARIRVF
                     ELGRVFLRDPAVADGPLEVAGVRQPLRIAAAASGPALEEQWGSPTRPVDLFDLKSDLE
                     TLAAPRALRYVAAAHPAFHPARSARVQMAGKDIGWIGELHPQWLQKYDISHGVVCFEV
                     EVGALLEAPLPQPSVPSRFPPVSRDIALLVNADITVQALLDAIVGETPAIVQDVRLFD
                     LYQGPNLPQGKKSLAFRVVMQDTERTLTDAEADAARDALVALWGRRFAATLRA"
     gene            complement(9409..10425)
                     /gene="pheS"
                     /locus_tag="AC16O9_03605"
     CDS             complement(9409..10425)
                     /gene="pheS"
                     /locus_tag="AC16O9_03605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6515735.1"
                     /translation="MQDVNAIVERALADFRAAADPASLENAKARYLGKSGELTALLRG
                     LGALAPEARKDAGARINAAKAAIEQALDARRGELSGARLQARLAAESIDVTLPGRGRG
                     RGGIHPIIRTWQRVEQIFGSIGFDVADGPEIENDWYNFTALNNPENHPARSMQDTFYV
                     DLKDAQGLPLLLRTHTSPMQVRYARMHSPPIKVIAPGRTFRVDSDATHSPMFHQVEGL
                     WIDEGISFADLKGVYTDFLRRFFESEDLEVRFRPSYFPFTEPSAEIDLQFASGPHKGR
                     WLELSGAGQVHPQVVRNFGLDAERYIGFAFGSGLERLTMLRYGIDDLRLFFDGDLRFL
                     RQFA"
     gene            complement(10448..10807)
                     /gene="rplT"
                     /locus_tag="AC16O9_03610"
     CDS             complement(10448..10807)
                     /gene="rplT"
                     /locus_tag="AC16O9_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR6515736.1"
                     /translation="MPRVKRGVTARARHKKVLKQAKGFRGRRGNVYRVAKQAVMKAGQ
                     YAYRDRRNRKRTFRALWIARINAAVRELGMSYSAFMSGLKRANIDIDRKVLADLAVAD
                     KPAFAKIASQVKASLAH"
     gene            complement(10817..11017)
                     /gene="rpmI"
                     /locus_tag="AC16O9_03615"
     CDS             complement(10817..11017)
                     /gene="rpmI"
                     /locus_tag="AC16O9_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR6515737.1"
                     /translation="MPKVKTKKSAAKRFKIRSSGGIKRSQAYMRHILTKKSTKRKRHL
                     RGTTNVHDSNKRQVRAMLPYAS"
     gene            complement(11103..11684)
                     /gene="infC"
                     /locus_tag="AC16O9_03620"
     CDS             complement(11103..11684)
                     /gene="infC"
                     /locus_tag="AC16O9_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR6515738.1"
                     /translation="MALERSQRINAEITAPQVRLVGENAEQLGIVPVAEALRLAEERN
                     VDLVEIAPMAQPPVCKLMDYGKFRYREQKKAHEAKLKQKQIQVKEIKFRPGTDEGDYK
                     IKLGKLIQFLEDGDKAKVTLRFRGREMAHQEFGVRLLERVRADLQEVGIVEQFPKLEG
                     RQMVMVLGPKKKGVPPKAKPKAKKDAAAVKPAA"
     gene            complement(11725..13629)
                     /gene="thrS"
                     /locus_tag="AC16O9_03625"
     CDS             complement(11725..13629)
                     /gene="thrS"
                     /locus_tag="AC16O9_03625"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR6515739.1"
                     /translation="MPNITLPDGSVKSFPGPVTVAEVAQAIGAGLARAALAGKVDGRL
                     VDTGHRIERDARLAIVTERDPEGLEVIRHSTAHLLAQAVKELYPEAQVTIGPVIENGF
                     YYDFAYKRPFTPEDLAAIEKRMAEIAKKDLPVTRRELPRDEAVKLFHGLGEAYKAEII
                     AAIPDKEAISLYGQGDWLDLCRGPHVPSTGKLKVFKLMRVAGAYWRGDSKNEMLQRIY
                     GTAWAKKEDQDAYLKMLEEAEKRDHRRLGTQLDLFHMQEAAPGLVFWHPKGWALWQQV
                     EQYMRRVYRDNGYQEVRAPQILDKSIWEKTGHWDNYRENMFTTSSENRDYAVKPMNCP
                     GHIQIYNKGVRSYRELPYRLGEFGACHRNEPSGALHGLMRVRGFTQDDGHIFCTEDQV
                     MQECVQYTALLQKVYADFGFADIIYRVATRPEKRIGDDAAWDKAERALIESLERSGVA
                     FQVTPGEGAFYGPKIEYSLKDAIGRVWQCGTMQVDFFMPERLGAEYVGEDNARHTPVM
                     LHRAIVGSMERFLGILIEHFAGAFPLWLAPEQVAVLNISEHQADYAAGVAAALRKAGF
                     RAVEDLRNEKISYKIREHSLQKLPYQVVVGDKEKQAGTVAVRTRGGEDLGAMSLDAFI
                     ARLRSEAKAQ"
     gene            complement(13635..13711)
                     /locus_tag="AC16O9_03630"
     tRNA            complement(13635..13711)
                     /locus_tag="AC16O9_03630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13675..13677),aa:Val,seq:gac)
     gene            13742..14194
                     /locus_tag="AC16O9_03635"
     CDS             13742..14194
                     /locus_tag="AC16O9_03635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR6515740.1"
                     /translation="MDLILWRHAEAEDGPPDLERRLTPRGHKHAAHVAGWLLQRLPAK
                     FVVLASPARRAQETALALGVPLRTVASLAPGAAVRDILAAAEWPERKSAVVVVGHQPD
                     LGRTAAFLLSGSEAPWSLKKGGAWWLSSRTREDAAQVVLRAVVAPDFV"
     gene            14221..15129
                     /locus_tag="AC16O9_03640"
     CDS             14221..15129
                     /locus_tag="AC16O9_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6515741.1"
                     /translation="MEEVNPTLAQLRDWLAVPWFNLGDTPISAGRLLGLVVIVVAVWW
                     LASLLEAGIRRVAMQRSGHTESTPSVYAWSRVLRYTVWIIGTLVGLNYIGLDLTSLAL
                     LGGAIGVGIGFGLQNIFSNFISGVIILLERTLKVGDYVDLQSGVRGHVREIGMRYTRI
                     TTNDEVDIIVPNSEFINGRVTNWTFETRVRRMRVPFGVAYGSDKDKVKAAALRAAQRV
                     KGTVSEPGREPDAWLVGFGDSSLNFELVVWIGPDLITRPGRTEAAFLWAIEDELRAAG
                     IEIPFPQRDLHVRSGTLNVKMEPERR"
     gene            complement(15126..16643)
                     /gene="ppx"
                     /locus_tag="AC16O9_03645"
     CDS             complement(15126..16643)
                     /gene="ppx"
                     /locus_tag="AC16O9_03645"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HIR6515742.1"
                     /translation="MAHQETLAAVDLGSNSFHLQIGRVVEGQIYPLDSLREVVRIGAG
                     LTADKRIDRATQARALAALRRFGERLRGLPRGAVRAVGTNTLRVAKNAPQFLREARQE
                     LGFPIEVIAGREEARLIYLGVAHAMPPAPHRRLVVDIGGGSTEFVIGAGVEPQLTESL
                     YMGCVSYSLKYFPDGRVDKARLKAAELAARQELAGIALAYRTLGWQEAVGSSGTARSI
                     ENILLENGYAEQGITREGLERLRSLLVKHERADPDRIAGLRPNRAPVLPGGLAIMSAV
                     LEELGIERMAVSEGALRHGVLYDLLGRVQHRDMRAATVAQFGRRYHVEAAQAERVRRL
                     ALAIYDALAPGREGEDDADRQMLAWAAALAEIGLSIEHAQYHKHSAYVLQNADMPGFS
                     RMEQQRLARLVLAHRGKLRKMQDAGLEASDWPLVFALRLASLILRRRADVKLPLLRVT
                     GDDSGFAIELAQSWLEDNPLSAAALEDEAGHWKSVGMRLAVSGLSDRKVSVVNRG"
     gene            16727..18808
                     /gene="ppk1"
                     /locus_tag="AC16O9_03650"
     CDS             16727..18808
                     /gene="ppk1"
                     /locus_tag="AC16O9_03650"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6515743.1"
                     /translation="MKRDLPTAHFLNRELGILAFNRRVLAQAADDKVPPLERLCFLTI
                     VSSNLDEFFEIRVAGLKEQIQLNLPEPGPDGKGPQEVFAAVSREARAIVAEQYRLLNE
                     VLLPALADSGVVFPRREAWTPAQRHWIREYFFRELLPVLTPIGLDPAHPFPKVLNKSL
                     NLAVELVGRDAFGRSSRAAIVQAPRALPRVIRLPRDIGGAPFAFVFLSSILHAHVEEL
                     FSGMKVLGCYQFRVTRNSDLFVDEEEIKNLHTALKGELLHRHFGNAVRLEVADNCSQA
                     MIDFLLQQFSLDEEDLYRVEGPVNLYRLREVPDQVERPDLKYPPFQPGLPAPLEARSD
                     LFELLKKQDVLLHHPYQSFAPVIEFIRTTAADPGVIAIKQTVYRTGTDSELMEILIDA
                     ARRGKEVTVVVELMARFDEEANLNWAARLEEVGAHVVYGVVGHKTHAKMALIVRREEL
                     DGRKVLRRYAHLATGNYHPRTARQYTDFGLLTANDELCADVAEVFKQLTGLGRAGRLR
                     HVWQSPFTLHKRVVEAIRNEARIAREGRPARIVAKMNALLDPQVIDELYLASQAGVRI
                     DLIVRSVCALRPGIDGLSDNIRVRSIVGRFLEHTRVFFFLADGAENVYLASADWMGRN
                     FFRRIELCFPVLDPALKRRVMREGLQPYLDDNVQAWVMDSDGVYTRARPRRGRPRSAQ
                     EELLFTLATPS"
     gene            18810..19184
                     /locus_tag="AC16O9_03655"
     CDS             18810..19184
                     /locus_tag="AC16O9_03655"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR6515744.1"
                     /translation="MAQPQPGATGLRRILNATRYSLEGLAAAVKHEAAFRQELLLAAV
                     LVPLGLWLGADGLERALLAGSVLFVLVVELLNSAVEAVVDRASPEHHELAKRAKDYGS
                     AAVMLALGLAGLVWVVLLLPRL"
     gene            complement(19186..19773)
                     /locus_tag="AC16O9_03660"
     CDS             complement(19186..19773)
                     /locus_tag="AC16O9_03660"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR6515745.1"
                     /translation="MQRLTLGAVALALPLAATAAPESYTLDPYHTYPHFAVDHLGVST
                     MWGRFDRSSGKFTIDRAAKKGSLDLTVETASLSTGDNDKGARPRSRDEHLRGADFFNV
                     AEFPRMTFKAGDVKFSGDSPTEVSGELTLLGVTKPLTLKIERWVCKDHPFSKKPMCGG
                     NASGSLKRTDFGMKYGVPAVGDEIRLYVEFEGLKD"
     gene            complement(19809..20648)
                     /locus_tag="AC16O9_03665"
     CDS             complement(19809..20648)
                     /locus_tag="AC16O9_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515746.1"
                     /translation="MEAALALLQTALGNPLVQSSALPLLAGLACAALLYPLRAEGLAA
                     IAGFLAAVFAIGNFDLEPLTAPRKVVLTGAAAALVGALYDLAFRPTRFSAPLLGAIFG
                     GAALWVFATVLRQKAPLDLALLGAGVVALTVWLVSATVTLQHDAARAGAAGLMLGLGA
                     GVSAVFGASALIGQYGMALGAACGGFLLLLLALGPRVGAGHALTLTVSVLSALLAAAS
                     VLLAQVPWVALAALALVPLAARLPLPASPLWMQPVLAGVYCAVPAAGACALAWLQSRG
                     WTL"
     gene            complement(20650..22659)
                     /locus_tag="AC16O9_03670"
     CDS             complement(20650..22659)
                     /locus_tag="AC16O9_03670"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR6515747.1"
                     /translation="MVSVAQLHLQTSATPLEPLTEGLAPEERALVAQALEFAGPLYEG
                     QLLSTGEPVWPHALGLAASLAAIGVDPAARAAGVLFAAPKYLAAGDALGARFGAEIAA
                     LAAGAEKLYQLRVLTRATAAQQSEALRKMVLGMVEDVRVVLIRLASRTQTLRWFAKHP
                     SAERAAHARETLDIYAPLANRLGVWQLKWELEDLSFRFLEPETYQRIARMLDERRVER
                     ERYIAQAMATLERELAAAGVAGEVSGRPKHIYSICNKMRLKGVDFEQLHDVRALRVIV
                     PEVQDCYAALGVVHNLWQPIEREFDDYVSRPKENLYQSLHTAVLGPGGKTLEVQIRTR
                     EMHRHAELGVAAHWRYKESGRTTRADTAHDDKIAWLRQLLAWRDEVVDRGTWAERTAA
                     AALDDTIYVVTPQGRVVDLPAGATPVDFAYALHTDIGHRCRGAKVDGRIVPLDTPLKS
                     GQRVEITVAKTGGPSRDWLNAERGFLKTSRARAKVRHWFNLQEGGREPEPPPRPKLVP
                     LPAPTAPAKKGRAKAGDGGILVVGMDRLLTQIARCCKPVPPDAIRGFVTRGRGVSIHR
                     ADCASLARLAQADSARLLDAQWGAAAGSYGVDMVVTARDRPGLLRDIGDALAREHINV
                     TAVRTQSRDDLATMRFSFEVADLAQLKRASAAVRDVSGVLRVARG"
BASE COUNT         3262 a         8003 c         7779 g         3652 t
ORIGIN      
        1 gaggggccgc gcgactacgc cgagcgcgcc gcggcgcgcc tgccgcaggc cgccgcggcg
       61 atccgcgcca ttgccgagct ttacatcggc gcgcgctacg gcagcgagcc ggcgcgcggc
      121 gccgagctcg cgcggcgcgt gcgcgagctt cgcctggcat gagcgcgcgc cgcctcctgc
      181 tcgcgctgct cgtcgccgcg ctcgccgcga ctgcgcgcgc cgaggaggac gagacggccg
      241 cgccttacgc cgagcgcacg gacgtgcagg gcttcatccg cgagctggtg gcgcggcacg
      301 acttcaacgc cgaggagctg gcgctggtgt tcgcgggcgc gcggcgcgag gaagccgtgc
      361 tcaccgccat gcgcgcgcag ccgcggcaag cggactcgtg ggacgcctat cgcgccctgt
      421 tcgtgaacga gcgccacctg cgcgccgggc tcgagttctg gcgggcgcac cgcccggtgc
      481 tggcgcgcgc gcgccgcgct tatggcgtgc ccgaggagat catcgtcgcc atcatcggca
      541 tcgaaacctt ttacggccgc aacaccgggc gctggcgcgt catcgacgcg ctggcgacgc
      601 tcgctttcga ctacccgccg cgcgcgccgt tcttccgcgg cgagctggag aactacctgc
      661 tgtttgcgcg tgagctgggg ctcgacgtgt tcgcggtgaa gggctcgtac gcgggcgcca
      721 tcggcatccc gcagttcatg cccggcagct acctgcgtta cgcggtggac ttcgacggcg
      781 acggcgccac cgacctgcgc ggcaatccgg ccgacgccat cggcagcgtc gccaacttcc
      841 tcaataagca cggctggcag cgcggcgcgc cggtgcagct cggcgcacgc gtgactggcg
      901 acggccacga gccgctcgtc gccggcggcc cgctgccgcg gcatacgctc gccgagcttg
      961 ccgatgccgg cgtcgaggcg cgcggcgcgc ccttgccggc cgagacgcgc gccgtgctgc
     1021 tgcgcctgga aacacccgag cggcccgcgg actaccgtct cggcttgcag aacttctatg
     1081 tgctgacgcg ctacaaccgc agcgtgttct acgcgctgtc ggtggcggac ctcgccgagc
     1141 agctgcgcgc cgcccggcgc aaggcgcgct agcgcccgct caggacggcc gctcgcccgg
     1201 caggtagcgc aggggatcga ccggcttgcc cgacttgcgg atctcgaagt gcagcttggg
     1261 ccggtcggcg tcgctgtcgc ccatctcggc gatgcgctgc ccgcgcgcca cgttctggcc
     1321 ttccttcacc aggatctcgc ggttgtgggc gtacacgctg ctgaagccgt tgtcgtggcg
     1381 gatgacgatc agcttgccga agccacggat gcccgtgccg gtgtaaagca cctgcccggc
     1441 cgccgcggcc agcaccggat cgcccggctt gccgccgatg tccacgccct tgctgctcgg
     1501 ctcgctgaac ttggcgagca ggccgccgcg cgccggccag atgaaatcga gcgactcggg
     1561 gtcgcggtcc accgccgctg tcggcggctc gggcttggcg ggcgcggcgc tgggcgcgac
     1621 cgcgacgggc ttcaatgtcg gcgccactgc cgccgcatcg cctctcgaca gcagggccag
     1681 gttctcctcg gagtagggca gccgcagcgc cttgggcgcg accttggtgc tgccgtcgcc
     1741 gcggccctgc tgcgccgtgc cggcgccgtc gagcgggcgc gcctgcagcg gtgcctggcc
     1801 gcgtgcggcg cccacctgca ccgacgtctc gccagcgggc ggggtcacgc gcagcacctg
     1861 gcccaccgaa atcttggtgg gatcgtcgag ctggttccag cgcgccacgt cgcggtagtc
     1921 cgcgccgtgc tcgagcgcga tgctgtagag cgtgtcgccg cgcttgatca tgtagtgccc
     1981 gggcggcacg ccggcggcga ccgccggcgg cggcggctcg ggctcgggcg cgggcttgag
     2041 cgccggcggc ggccggatgt cggtcaccgg cgcctgcacc ggttcggaag cgcagccgcc
     2101 cagggccgcc agcgccgcgg cgaggagcga cgcgctgcat cgcgatgcga atgtcgtcat
     2161 agttttcccg tctcgagagg cacgaacagc acctcgtcga gtgtcgtttc cgaaatgccg
     2221 cgcgccttgc gctccagcag cgccaggcgc tgcgtgccgt gcggcgcctg cggcggcttc
     2281 agcggcagca ccattctgcc acccggcgcc agctgctgta aaaggacttg cggcaccgca
     2341 cgcgccgcgg ccgccacgat gatcgcgtcg aagggtgccg cctcctgcag gccgagattg
     2401 ccgtcgccat gggtgagccg caggttcgac agccgcaggc cacgaagatt cgcgcgtgcg
     2461 cgctccagca aagcgcgcac gcgctccacc gagtacacct cgcgcgcgag ctgcgccagc
     2521 accgcggcct ggtagccgca gccggtgccg acctccagca cgcgcgccgg cacgccgttg
     2581 tcgccgatca gcgactcgat catgcgcgcc accacgtacg gctgcgagat ggtctggctg
     2641 aagccgatcg gcagcgcggt gtcctcgtag gcgcggctcg ccagcgcttc gtccacgaac
     2701 aggtgccgtg gcacggcgcc catcgccgcc agcacgcgct catcgcggat gcccttctcg
     2761 cgcaggcgct ccaccatgcg caggcgcgtg cgctgcgagg tcatgcccgt gccggcggcg
     2821 tcgcgcgggc cgtgcgcctt catgccgcga gccaggcgcg caccggcgcg agctgcgccg
     2881 gatgcgtgag gtccacctgc agcggcgtca ccgacacgcg gccgctcgcc acggcgtgga
     2941 agtcggtgcc cgggccggcg tcggcggcct cgcccgccgc gccgatccag tacgcctgct
     3001 cgccgcgcgg ggtgcgcatc ttcaccaccg gctcggcctt gtggcggcgg ccgagccgcg
     3061 tcacctcgac ggcgccgagc gcctccggcg ccacgtcggg cacattcacg ttgagcagca
     3121 ccggctggcc gagcggcgcg cgcccgaagc gctccaccag ccgctgcgcg acgtcgactg
     3181 cggtggcaat gaagcggccc tccttgcttg ccagcgacac cgcgagcgac gggatgccga
     3241 gcaggaaccc ctcggtggcc gccgccacgg tgccggaata gatcgtgtca tcgcccatgt
     3301 tcgcgccaag gttgacaccg gagacgacga tgtcgggcag gaagtcgagc acgcccgtga
     3361 ccgccaggtg cacgcagtcg gtgggcgtgc cgttgacgaa gaaaaagccg ttcggcgcgc
     3421 ggcgcaccga gagcggccgg tcgagggtca gcgaattcga ggcgccggag cggtcgcgct
     3481 ccggcgcgaa caccgtgact tcgccaagcg cgcccaggcg ctcggcaagc tgcgcgatgc
     3541 cgggcgagaa gtagccgtcg tcgttcgaca ccaggatgcg catggcctag aaggagcgcg
     3601 gcaggcccag cacgtgctcg gcgacgtagg acaggatcag gttggtggaa atcggcgcca
     3661 ccaggtaaag gcgcgactcg cggaacttgc gctcgacgtc gtactcggca gcaaagccga
     3721 agccgccgtg cgtctgcagg caggcgttgg ctgcctccca ggatgccttg gcggcgaggt
     3781 gcttggccat gttggcctcg gccccgcaat gctggtgcgc gtcaaagagc gcggcggcct
     3841 tgtagcgcat caggttcgcc gcttcgacct ccatgtaggc ctccgcgatc ggaaactgga
     3901 tgccctggtt ctggccaata gggcggtcga acaccacgcg gtccttggcg tagttcgtcg
     3961 cgcgcccgac gaaccagtag ccgtcgccga tgcattcggc ggcgatcagg atgcgctcgg
     4021 cgttcagccc gtcgaggatg tagcggaagc ccttgccctc ctcgccgatc aggttttccg
     4081 ccgggatctc gaggttgtcg aagaacacct cgttggtctc gtggttcacc atgttgcgga
     4141 tcgggcgcat cgtcaggccc ttgccgagcg ccgctttctt ctccaccagg aacaccgaca
     4201 tgccgtcgga tttcttgttc acctcggcga gcggcgtagt gcgcgcgagc aggatcatca
     4261 agtcggagtg ctgcaggcgc gaggtccata ccttctggcc gttcaccacg tagcgatcgc
     4321 ccttcttcac ggccacggtc ttgatcttcg tcgtatcggt gccggcggtg ggctcggtca
     4381 cgcccatcgc ctgcatgcgc agctcgccgc tcgcgatctt cggcaggtat ttccgcttct
     4441 gcgcctccga gccgttcctg agcagcgtgt tcatgttgta catctggcca tggcagcagc
     4501 ccgggttgcc gcccgagcgc gagatctctt ccatgatcac ggtggcctcg gtgagcgaga
     4561 ggcccgagcc gccatactgc tcgggaatca gcgccgccat ccagccggca ccggtcagcg
     4621 cgtcgacgaa cgcctccggg taggcgcgcg cctcgtccac cttctgccag taggcggaat
     4681 cgaactgggc gcacagggcg cgcacgccct cgcgcagctc ggcgtgggca gtatcggcta
     4741 cagcggatcc catgtgaata cgtcctgagt gcggtcgagg gggaagaagc tctggcgcag
     4801 cgccggcatg ccgtgcgctg cgatctcctg cggcgtccag ccctcggcgc ggtgcaccga
     4861 gcgcactggg cggctctgcg acatgaggaa cagctcgttc gcccgcactg cgaacacctg
     4921 tcccgagaca tcgtgcgcgg cgtcgctcgc caggtagacg gcgagcggcg cgatcttggc
     4981 ggtctccatc accttcatct tctcgacgcg cgccttctgc tccggcgtct cggtcggaat
     5041 cgagccgatc atgcggctcc aggcaaaggg cgcgatgcag ttcgagcgca cgccgtagcg
     5101 cgccatgtcg agcgcgatcg acttggacag cccgacgatg ccgagcttgg cggcggcgta
     5161 gttggcctgg ccgagattgc cgatcaggcc cgaggtcgag gtcatgtgca cgtagcagcc
     5221 cgagttctgg ctcttgaagt agggcgccgc ggcccgcgac acgtagaacg agccgttcag
     5281 gtgcacatcg atcaccgagc gccactcctc caccgacatg ttgaagaaga agcggtcgcg
     5341 caggatgccc gcgttgttca ccacgcaatc gaggtggccg taggcgtcga tcgacgcctt
     5401 caccaggcgg ttggccgact cccagtcggc gacgctgtcg gcactggcga tcgcctcgcc
     5461 gccgcgcgcc ttgatctcgt cgacgacctt ctgcgccggg ccggcgcttg cgccctcgcc
     5521 ggccaccgag gtgccgaggt cgttgaccac caccttggcg ccatgcgcgg cgaaggcgag
     5581 cgcgaaatcg cgcccgatgc cgccccccgc gcccgtcacc agcacgatct tgtccttcac
     5641 catgttgtct ctcttgcgtg cgtcagggtg cgcaaaactt taacagatat ttgcgctcgt
     5701 tccggcgccg cacgtcttgc agacaatgac accgcgcgcc gcgggattgg agttgaatcc
     5761 tgcaaaggcc atgcgcactg cccagaccgc tttcacttcc cgcgcccgac ccgatcggtt
     5821 cgtttggcag gtctcggctg cgcggagcgc aggcaaggtg ctcgcgcccg agtcgctcga
     5881 gctggacctg ttcctgttcc tcgcctgcgc caagctggcg gcgttcgagg cggtgacgtg
     5941 caactgaagg aacctgacag cgccgccttc ggcgccgagg cccttccggg cgggctgcgc
     6001 gagatcaacg cgcagcgtcg cggcgaaccg ctggcgccag tgtccgcccc tgcgccaacg
     6061 cagcccgcgt cgctggtggg gctgatcgcc ctcgcgaaac gctacttcgg ctggggcacc
     6121 acgcgcaggt agagcttcag cgtcctgaag ccctgtgggc acttcttctt cgtatcctca
     6181 cccgagcgcg acgtcggcgt gtcgcgcctg tcgcgctcgg gcttgaagac ggaaaaccgg
     6241 gattggtcgg ggcggcgaga ttcgaactcg cgaccacctg caccccatgc aggtacgcta
     6301 ccaggctgcg ctacgccccg agccgaggga ttctatcagg cgcgcaacgc ttccagcagc
     6361 gcgcgtagct cgcgcctgat cgccgccagg tcgccgcccg cggcggcggc gcttggcgcc
     6421 ggctcgctgc gctcgtcggc gccgtgcgcg gcgttgtcga gccggttgcg cgcgccgctg
     6481 atggtgaagc cctcctcgta cagcagctcg cggatgcggc ggatcagcag cacttcgtgg
     6541 tgctggtagt agcgccggtt gccgcggcgc ttgacgggtt tcaactgcgt gaactcctgc
     6601 tcccagtagc gcagcacgtg cggtttcacg ccgcacaact cgcttacctc accgatggtg
     6661 aagtagcgct tcgccgggat cggcggcagg tcagctttct tgctgtggtt gcggctttcc
     6721 atggcgtgcc ttctccacca tcgccttcag cttctggctg gcgtggaagg tgaccacgcg
     6781 gcgggcgctg atggggattt cctcgccggt tttggggttg cggccagggc gttgcggctt
     6841 gtcgcgcagt tggaagttgc cgaaccccga cagcttgacg ctctcgccgc gctcgagggc
     6901 cccgcggatt tcctcgaaga aagcttccac catgtccttg gcctcgcgct tgttcaggcc
     6961 gaccttctcg aacagcaggt cggccagttc ggccttggtc aaagtcatca caactccctg
     7021 atttgtatat ttatgcgcga agtgtcgcgg cgaaacgacg gccccacagc gcaaccagcg
     7081 catcccgcgc cgcgtcggcc tccgcatccg tgagggttcg ttcagtatct tgcataacta
     7141 cccggaaagc aaggcttttt ttcccttggg gaaggttcgg cccctggtac aggtcaaaca
     7201 gccgcacgtc ctgcacgatg gccggggttt ctcccacgat cgcatccagt agcgcctgaa
     7261 cggtgatgtc agcgttcact aacaaagcaa tgtcgcgaga cacgggcggg aacctggagg
     7321 gtaccgaggg ttgggggagg ggtgcctcca agagcgcgcc gacctcgacc tcaaagcaca
     7381 caaccccatg agaaatatcg tatttctgca gccactgggg gtgtagttcc ccgatccagc
     7441 cgatgtcttt tcccgccatt tggacgcggg cggaacgcgc gggatggaac gccggatgcg
     7501 cggcagccac gtagcgtagg gcgcgggggg cggcgagggt ctcgaggtcc gacttcaggt
     7561 cgaagaggtc cacgggccgc gtggggctgc cccactgctc ttccagcgcc ggcccggacg
     7621 cggccgcggc gatgcgcagc ggctggcgca cgccggcgac ttcgagcggc ccgtcggcga
     7681 cggccgggtc gcgcagaaac acgcgcccga gctcgaacac ccggatccgc gccagcttgc
     7741 gcgccgcgtt gtagcgcacg ttggcgagca gcccaccgag cagcgtgctg cgcatgaccg
     7801 agagctggct ggcgatgggg ttgagaagcc gaatcgggtt cgacgcgccc gccaggtccg
     7861 cctcccactg cggctcgacg aagctgaagg tgatcacttc ctggtagtca cggccggcca
     7921 ggcgctcgcg cagcgcgtgc accgggcggc gcgcctccgg cgtcgccgcc atgcgcgcgg
     7981 gcgcaagggg cggcagcgcc gggatgcgct cgaagccatg cacgcgcgcc acctcctcga
     8041 tcaggtcttc ctcgatctcc aggtcgaagc gaaacgaggg cggcgcgacg gtgaaagcgc
     8101 cgcgctggcg ccggctcggc agccccaaac gcttgaacgc ccgcgccatg tccttttccg
     8161 cgaccggcat gccgatcact ttctgcgcgc gcgcgacgcg catgcgcacg ggctttctct
     8221 tgggcaggcg cttgaccaga tcgaccgtcg ggcccggctc gccgccgcag atctccagga
     8281 tcagctgcgt ggcgcgctcg atgccggcga cgttgttgcc gaagtcgacg ccgcgctcga
     8341 aacggtgcgc ggcgtcgctc gtgaaattga agcgccgcgc gcggcccgcc accgcgtcgg
     8401 ggaagaagaa cgccgattcg aggaacaggt tccgggtcgt ggtctcggcc ttggtcgact
     8461 ccccgcccat gatgccgccc aggccgatcg gacccgaatc gtccgtaatg cacagcacgt
     8521 ccgcgtcgac ctcgacttcc tgcccgttca gcaggagcac cttctcgcct ttgcggcccc
     8581 agcgcacgtc gatcgggccg cgcagcttgt cctggtcgta cacgtgcagc ggccggccga
     8641 gctcgagcat cacgtagttg gtgacgtcga ccagcgccga gatcgagcgc tgccccgcgc
     8701 gctccagccg ctggcgcatc cagtgcggcg tcggcgcggc ggcgttcacg ccgcggatca
     8761 cgcgcccggc gaagcgcccg cagcccgcgg cgtgcgtgac gccgaacggg tgcttcgcct
     8821 tgctgcgcgc tgcgaccggc ttcggcctgg gcgccgtcag ctttgccccg gtcagcgcgg
     8881 cgacctcgcg cgcgatgccg aggatcgaca ggcaatcggc gcggttcggc gtcagcttga
     8941 acgtgaacac acgctcgtcc aggtcgagcg ccttgcgcac gtcctgcccc gccttcgccg
     9001 tcaccggcag ctcgagcagg ccggaatggt cgtcggagag tccgagctcg cgtgccgagc
     9061 acagcatgcc ctggctctgc acgccgcgca gctcggccgc cttgatctcc aggcttccga
     9121 gcctcgttcc cacggtggcg agcggggcct tcatgcccgg gcgtgcgttt ggcgcaccgc
     9181 acaccacagc gagcttctgc tggcccgtgc tcacggtgca gaccgtgagc ctgtcggcgt
     9241 tcgggtggcg ctcgacctgc aggatctcgc cgaccaccac gccgctgaag ggcggcgcta
     9301 cgggcacgca ggcctcgacc tccagccccg acatcgtcag cagctgcgcg agcttgtccg
     9361 ccttcatcga cggatcgcag aagctgcgca gccagcgctc ggggacgttc atgcgaactg
     9421 ccggaggaag cgcaggtcgc cgtcgaagaa caggcgcaga tcgtcgatgc cgtagcgcag
     9481 catggtcagg cgctcgagac ccgagccgaa ggcaaagccg atgtagcgct cggcatccag
     9541 cccgaaattg cgcaccacct gcggatgcac ctggccagcg cccgagagct cgagccagcg
     9601 ccccttgtgc ggcccggacg caaactgcag gtcgatctcc gcggagggct cggtgaacgg
     9661 gaagtacgac ggccggaagc gcacttccag gtcttcggac tcgaaaaagc ggcgcaggaa
     9721 gtccgtgtac acgcccttca ggtcggcgaa gctgatgccc tcgtcgatcc acaggccctc
     9781 gacctggtgg aacatgggcg agtgcgtcgc atccgaatcg acgcgaaagg tgcggcccgg
     9841 cgcgatcacc ttgatgggcg gtgagtgcat gcgcgcatag cgcacctgca tcgggctggt
     9901 gtgcgtgcgc agcagcaacg gcaggccctg cgcgtccttc agatcgacgt agaacgtgtc
     9961 ctgcatcgag cgcgccgggt ggttctcggg gttgttcagc gcggtgaagt tgtaccagtc
    10021 gttctcgatc tcgggaccgt cggcgacgtc gaagccgatc gaaccgaaga tctgctcgac
    10081 gcgctgccag gtgcgaatga tcgggtggat gccgccgcgg ccgcggccgc ggcctggcaa
    10141 ggtgacgtcg atcgattctg cggcgagccg cgcctgcagc cgcgcgccgg agagctcgcc
    10201 acggcgcgcg tccagcgcct gctcgatcgc cgctttggcg gcgttgatcc gggccccggc
    10261 gtccttgcgc gcttcgggtg cgagcgcgcc cagcccgcgc agcagcgccg tcagctctcc
    10321 gctcttgccg aggtagcgcg ccttggcgtt ttccagcgac gcggggtcgg cggcggcgcg
    10381 gaaatccgcg agcgcccgct cgacgatggc attgacgtct tgcatccccg cgcctcccgc
    10441 aacggggtca gtgggcgagg ctggctttca cctggctggc gatcttcgcg aacgcgggct
    10501 tgtccgcgac cgccagatcg gcgagcacct tgcgatcgat atcgatgttg gcgcgcttca
    10561 gcccggacat gaaggccgaa tagctcatgc cgagctcgcg caccgcggcg ttgatgcgcg
    10621 cgatccacag cgcgcggaag gtgcgcttgc ggttgcggcg gtcgcggtag gcgtactgcc
    10681 ccgccttcat caccgcctgc ttggcgacgc gatagacatt gccgcggcgg ccgcggaagc
    10741 ctttcgcctg cttcaggacc ttcttgtggc gcgcgcgcgc cgtgaccccg cgcttgactc
    10801 ttggcatggc gcctccttac gacgcgtagg gcagcatcgc gcgcacctgg cgcttgttgc
    10861 tgtcgtgcac gttggtggtg ccgcgcaggt ggcgcttcct cttggtcgac ttcttggtaa
    10921 ggatgtggcg catgtaggcc tgcgagcgct tgatgccgcc cgaggagcgg atcttgaagc
    10981 gcttcgccgc cgacttcttg gtcttcactt tcggcatgac tcttccttta tagcctgacg
    11041 ccgggcggcc cgccgcgcgg gcgctttcac tgccgcggcc atcacttgta ttgccggtcc
    11101 gctcaggcgg ccggcttgac tgctgctgcg tccttcttcg ccttgggctt ggctttgggc
    11161 ggcacgccct tcttcttggg ccccagcacc atcaccatct gccggccctc gagcttcggg
    11221 aactgctcca cgatgcccac ttcctgcagg tccgcccgca cccgttccag gagccgcacc
    11281 ccgaactcct gatgcgccat ctcgcgcccg cggaaccgca acgtcacttt ggccttgtcg
    11341 ccgtcctcca ggaactggat cagctttccc agcttgatct tgtagtcgcc ctcgtcggtc
    11401 cctgggcgaa acttgatctc cttcacctgg atctgctttt gcttgagctt ggcctcgtgg
    11461 gccttcttct gctcgcggta gcggaacttg ccgtagtcca tcagcttgca gaccggcggc
    11521 tgcgccatcg gcgcgatctc caccaggtcc acgttgcgct cctccgccaa acgcagcgcc
    11581 tcggccaccg gcacgatgcc cagctgttct gcgttctcgc cgaccagccg cacttgcggc
    11641 gcggtgatct ccgcgttaat gcgttgcgat ctttcgagtg cgatgagtga caagctcctt
    11701 tacacgttga aaaaaacggc cgtgctactg cgctttcgcc tcgctgcgga ggcgtgcgat
    11761 gaaggcatcg aggctcatcg ccccgagatc ttcgccgccg cgcgtgcgca cggccaccgt
    11821 tccagcctgc ttctccttgt ccccgacgac cacctggtag ggcaactttt gcaggctatg
    11881 ttcgcgaatc ttataagaaa ttttctcgtt tcgcaaatct tccacggcgc ggaaaccggc
    11941 cttgcggagc gccgccgcga ccccggcggc atagtccgcc tggtgctccg agatgttgag
    12001 caccgccacc tgctccgggg ccagccagag cgggaacgcg ccggcgaaat gctcgatgag
    12061 gatgccgaga aagcgctcca tcgagccgac gatagcgcgg tggagcatca ccggggtatg
    12121 gcgcgcgttg tcttcgccca cgtactccgc gcccaggcgc tccggcatga agaaatccac
    12181 ctgcatggtg ccgcactgcc agaccctgcc gatggcgtcc ttcagcgagt actcgatctt
    12241 cggcccgtag aaggcgccct cgcccggggt gacctggaac gccactccgg atcgctcgag
    12301 cgactcgatc agcgcgcgct cggccttgtc ccaggccgcg tcgtcgccga tgcgcttctc
    12361 ggggcgggtc gccacccggt agatgatgtc ggcaaagccg aaatcggcgt acaccttctg
    12421 cagcagcgcc gtgtactgga cgcactcctg catcacctgg tcctcggtgc agaagatgtg
    12481 gccatcgtcc tgcgtaaagc cgcgcacgcg catcagcccg tgcagcgcgc ccgagggctc
    12541 gttgcggtgg caggcgccga actcgcccag ccggtacggc aactcgcggt agctgcgcac
    12601 gcccttgttg tagatctgga tgtgcccggg gcagttcatc ggcttcaccg cgtagtcgcg
    12661 gttctccgag gacgtggtga acatgttctc gcggtagttg tcccagtgcc cggtcttctc
    12721 ccagatgctc ttgtcgagga tctgcggcgc gcgcacttcc tggtagccgt tgtcgcggta
    12781 cacgcggcgc atgtactgct cgacctgctg ccacagcgcc cagcccttgg ggtgccagaa
    12841 cacgaggccc ggtgccgctt cctgcatgtg aaagagatcg agctgcgtgc cgaggcgccg
    12901 gtggtcgcgc ttctcggcct cctccagcat cttcaggtac gcgtcctggt cctccttctt
    12961 cgcccaggca gtgccgtaga tgcgctggag catctcgttc ttagagtcgc cgcgccagta
    13021 ggcgccggcg acgcgcatca gcttgaagac cttgagcttc ccagtcgagg gcacatgcgg
    13081 gccgcggcac aggtcaagcc agtcgccctg gccgtacagc gagatcgctt ccttgtccgg
    13141 gatcgcggcg atgatctcgg ccttataggc ctcgcccaga ccgtggaaca gcttcactgc
    13201 ctcgtcgcgg gggagctcgc gccgcgtcac gggcaggtct ttcttcgcaa tctcggccat
    13261 gcgcttttcg atggccgcca ggtcctctgg cgtgaacggg cgcttgtacg cgaaatcgta
    13321 gtaaaagccg ttctcgatca cgggcccgat ggtgacctgc gcctcgggat agagctcctt
    13381 caccgcctgc gccagcaggt gcgccgtcga gtgccggatc acctccagcc cctcggggtc
    13441 acgctcggtg acgatcgcga gcctggcgtc gcgctcgatg cggtggccgg tgtcgaccag
    13501 gcggccgtcc accttgccgg cgagcgccgc gcgcgcgagg cccgcgccga tggcttgggc
    13561 gacttcggcg acggtcaccg ggcccggaaa cgacttcacc gagccgtctg gaagcgttat
    13621 gttgggcaag atgttggtag gcgcgattgg actcgaacca acgaccccta ccatgtcaag
    13681 gtagtgctct aaccagctga gctacgcgcc tgatggaacg agcccgggat tctaactgaa
    13741 gatggacctg attctgtggc ggcacgccga ggccgaggac gggcctcccg acctcgagcg
    13801 ccgcctcacg ccccgcggcc acaagcacgc ggcgcacgtc gccgggtggc tgctgcagcg
    13861 gctgccggcg aagttcgtgg tgctggccag cccggcgcgg cgcgcgcagg aaacggcgct
    13921 ggcgctgggc gtgccgctgc gcacggtggc gagcctcgcc ccgggcgccg cggtgcgcga
    13981 catcctcgcc gccgccgagt ggcccgagcg gaagagcgcc gtcgtcgtgg tggggcacca
    14041 gcccgacctc gggcgcaccg ccgccttcct gctgtcgggg tcggaggcgc cgtggagcct
    14101 gaagaagggc ggcgcctggt ggctgtccag ccgtacgcgc gaggatgcgg cgcaggtcgt
    14161 gctgcgcgcg gtggtcgcgc ccgacttcgt ctagcgcccg catgctaggc tagacgcggc
    14221 atggaagaag tgaatccgac gctcgcgcaa ctgcgcgact ggctggcggt gccgtggttc
    14281 aacctgggcg acacgccgat cagcgccggc cggctcctcg ggctggtggt gatcgtggtc
    14341 gccgtgtggt ggctggcgtc gctgctcgaa gccggcatcc ggcgggtcgc catgcagcgc
    14401 agcggccaca ctgagagcac gccgagcgtg tacgcctggt cgcgcgtgct gcgctacacg
    14461 gtatggatca tcggcacgct ggtcgggctg aactacatcg gcctcgatct caccagcctc
    14521 gcgctgctcg gcggtgccat cggcgtcggc atcggcttcg gcctgcagaa catcttcagc
    14581 aacttcatct ccggcgtcat catcctgctc gagcgcacgc tgaaggtcgg cgactacgtc
    14641 gacctgcagt ccggcgtgcg cgggcacgtg cgcgagatcg gcatgcgcta cacgcgcatc
    14701 accaccaacg acgaggtgga catcatcgtg cccaattcgg agttcatcaa cggccgcgtc
    14761 accaactgga cgttcgagac gcgcgtccga cgcatgcgcg tgcccttcgg cgtcgcctac
    14821 ggcagcgaca aggacaaggt gaaggcagcg gcattacgtg ccgcgcagcg cgtgaagggc
    14881 accgtcagcg agcccgggcg cgagcccgac gcctggctgg tcggcttcgg cgactcgagc
    14941 ctcaatttcg agctggtggt ctggatcggg cccgacctca tcacgcgccc cgggcgcacc
    15001 gaggctgcgt tcctgtgggc gatcgaggac gagctccgtg ccgcgggcat cgagatcccg
    15061 ttcccccagc gcgacctgca cgtgcgctcg ggcacgctca acgtgaagat ggagcccgag
    15121 cggcgctagc cgcggttcac gaccgacacc ttcctgtcgg acaggccgct cacggcgagc
    15181 ctcatgccga ccgacttcca gtgcccggcc tcgtcctcga gcgccgccgc ggacagcggg
    15241 ttgtcctcga gccaggattg cgccagctcg atcgcgaagc cgctgtcgtc gccggtgacg
    15301 cgcagcagcg gcagcttcac gtcggcgcgc cggcgcagga tgagcgaggc gagccgcagc
    15361 gcgaagacca gcggccagtc ggaggcctcc agcccggcgt cctgcatctt gcgcagcttg
    15421 ccgcggtgcg ccagcacgag gcgcgcgagg cgctgctgct ccatgcgcga aaagcccggc
    15481 atgtctgcgt tctgcagcac gtacgcggag tgcttgtggt actgcgcgtg ctcgatggaa
    15541 aggccgatct cggccagcgc cgccgcccac gccagcatct ggcggtcggc gtcgtcctcg
    15601 ccctcgcgcc cgggcgccag cgcgtcgtag atggcgagcg ccagccgccg cacgcgctcg
    15661 gcctgcgccg cctcgacgtg gtagcggcgc ccgaactgcg ccacggtggc cgcgcgcatg
    15721 tcgcggtgct gcacgcgccc gagcaggtcg tagagcacgc cgtggcgcaa cgcgccctcc
    15781 gacaccgcca tgcgctcgat gccgagctcc tcgagcaccg cgctcatgat cgcgaggccc
    15841 ccgggcagca ccggcgcgcg gttgggccgc agaccggcga tgcgatccgg gtcggcgcgc
    15901 tcgtgcttta cgagcaggct gcgcaagcgt tcgagccctt cgcgcgtgat gccctgctcg
    15961 gcgtagccgt tctccagcag gatgttctcg atcgagcgcg ccgtgcccga ggagcctacc
    16021 gcctcctgcc agccgagcgt gcggtaggcg agcgcgatgc cggcgagctc ctggcgcgcg
    16081 gcgagctccg ccgccttcag ccgtgccttg tcgacgcgcc cgtccgggaa gtacttcagg
    16141 ctgtagctga cgcagcccat gtacagcgac tcggtgagct gcggctcgac gccggcgccg
    16201 atgacgaatt ccgtcgagcc gccgccgatg tccacgacaa ggcgcctgtg tggcgcgggc
    16261 ggcatcgcgt gcgcgacgcc gagatagatg aggcgcgctt cctcgcgtcc ggcgatcacc
    16321 tcgatcggga agccgagctc ctggcgcgcc tcgcgcagga actgcggcgc gttcttcgcc
    16381 acgcgcagcg tgttggtgcc caccgcgcgc accgcgccgc gcggcaagcc gcgcaggcgc
    16441 tcgccgaagc gccgcagcgc cgcgagcgcg cgcgcctgcg tggcgcggtc gatgcgcttg
    16501 tcggcagtga ggccggcgcc gatgcgcacg acctcgcgca gggaatccag ggggtagatc
    16561 tgcccctcga ccacgcggcc gatctgcagg tggaagctgt tcgagccaag gtcgactgcg
    16621 gctagcgttt cctggtgcgc catgggcgcc tactgtagct caggcggccg cgccaaggag
    16681 cgtagaatgg ccgttccgcg agccgctttc ccgcggcctc ccgcgcatga agagagacct
    16741 gcccactgcg catttcctga accgcgagct cggcatcctc gcgttcaacc ggcgcgttct
    16801 ggcgcaggcc gccgacgaca aggtgccgcc gctcgagcgg ctgtgcttcc tcaccatcgt
    16861 gtcgtcgaac ctcgacgagt tcttcgagat ccgcgtggcg ggcctcaagg agcagatcca
    16921 gctcaacctg cccgagcccg gccccgacgg caagggtccg caggaggtgt tcgccgcggt
    16981 gagccgcgag gcgcgcgcca tcgtcgccga gcagtaccgg ctgctgaacg aggtgctgct
    17041 gccggcgctc gccgactcgg gcgtggtgtt cccgcggcgc gaggcctgga ccccggcgca
    17101 gcgccactgg atccgcgagt acttcttccg cgagctgctg ccggtgctga cgccgatcgg
    17161 cctcgacccg gcgcacccgt tcccgaaagt gctgaacaag agcctgaacc tcgccgtgga
    17221 gctggtcggc cgcgatgcct tcggccgcag ctcgcgcgca gccatcgtac aggcgccgcg
    17281 cgcgctgcca cgcgtcatcc gcctgccgcg tgacatcggc ggcgcgccct tcgccttcgt
    17341 gttcctctcg tcgatcctgc acgcgcacgt cgaggagctg ttctcgggca tgaaggtact
    17401 tggctgctat cagtttcgcg tcactcgcaa ctccgacctg ttcgtcgacg aggaggaaat
    17461 caagaacctg cacactgcgc tgaagggcga gctgctgcac cggcacttcg gcaacgcggt
    17521 gcgcctggaa gtcgccgaca actgctcgca ggcgatgatc gacttcctgc tgcagcagtt
    17581 cagcctcgac gaggaggacc tgtaccgcgt cgagggaccg gtgaacctgt accggctgcg
    17641 cgaggttccc gatcaggtgg agcggcccga cctgaagtac ccgccgttcc agcccgggct
    17701 gccggcgccg ctcgaggcgc gcagcgacct cttcgagctg ctgaaaaagc aggacgtgct
    17761 gctgcaccat ccgtaccagt cgttcgcgcc ggtgatcgag ttcatccgca ccaccgcggc
    17821 cgatcccggc gttatcgcca tcaagcagac ggtgtaccgc accggcaccg actccgagct
    17881 gatggagatc ctgatcgacg ccgcgcgccg cggcaaggaa gtgacggtgg tggtcgagct
    17941 gatggcgcgg ttcgacgagg aagcgaacct caactgggcc gcgcgcctgg aggaggtcgg
    18001 cgcgcacgtg gtctacggcg tcgtcggcca caagacgcac gccaagatgg cgctcatcgt
    18061 tcgtcgcgag gagctcgacg gccgaaaggt gctgcgccgc tacgcgcacc tcgcgaccgg
    18121 caactaccac ccgcgcaccg cgcgccagta caccgatttc ggcctgctca ccgccaacga
    18181 cgagctgtgc gccgacgtcg ccgaggtgtt caagcagctc accgggctcg ggcgcgccgg
    18241 caggctacgc cacgtctggc agtcgccgtt cacgctgcac aagcgcgtgg tcgaggcgat
    18301 ccgcaacgag gcgcggattg cgcgcgaggg acggcccgcg cgcatcgtcg ccaagatgaa
    18361 cgcgttgctc gacccgcagg tgatcgacga gctctatttg gcttcgcagg cgggcgtgag
    18421 aatcgacctc atcgtgcgca gcgtgtgcgc gctgcgcccc ggcatcgacg ggctgtcgga
    18481 caacatccgc gtgcgctcga tcgtcggccg gttcctcgag cacacgcgcg tgttcttctt
    18541 tctcgccgac ggcgcggaga acgtgtacct cgccagcgcc gactggatgg ggcgcaattt
    18601 cttccgccgc atcgagctgt gctttcccgt gctcgacccg gcgctcaagc gccgcgtgat
    18661 gcgcgagggc ctgcagccct acctcgacga caacgtgcag gcctgggtga tggacagcga
    18721 cggcgtctat acccgcgcgc gcccgcgccg cggccggccg cgctcggcgc aggaggagct
    18781 gctgttcacg ctggcgacgc cgtcctgaga tggcgcagcc gcaaccgggc gcgaccggct
    18841 tgcgccgcat cctcaacgcc acgcgctact cgctcgaggg cctcgctgcc gcggtgaagc
    18901 atgaggcggc gttccgccag gaactgctgc tcgccgccgt gctggtgccg ctcgggctgt
    18961 ggctgggcgc ggacgggctc gagcgcgcgc tgctcgccgg cagcgtcctc ttcgtgctgg
    19021 tcgtggagct gctcaactcg gccgtcgagg cggtcgtcga ccgcgcctcg ccggagcacc
    19081 acgagctcgc caagcgcgcc aaggactacg gcagcgccgc ggtcatgctc gcgcttggcc
    19141 ttgccgggct ggtctgggtc gtgctgctgc tgcccaggct ctgagtcagt cctttaagcc
    19201 ctcgaactcc acgtacagcc ggatctcgtc gcccaccgct ggcacgccgt acttcatgcc
    19261 gaagtcggtg cgcttcaggc tgcccgaggc gttgccgccg cacatcggct tcttgctgaa
    19321 gggatggtcc ttgcacaccc agcgctcgat cttcagcgtg agcggcttgg tgacgccgag
    19381 cagcgtcagc tcgcccgaga cctcggtggg gctgtcgccg ctgaacttca cgtcgcccgc
    19441 cttgaaggtc atgcgcggga actcggcgac gttgaagaaa tcggcgccgc gcagatgctc
    19501 gtcgcgcgag cgcggccggg cgcccttgtc gttgtcacct gtgctgagcg acgcagtctc
    19561 caccgtgaga tcgagcgagc ccttctttgc ggcgcggtca atggtgaact tgccgctcga
    19621 gcggtcgaag cggccccaca tcgtggacac gcccaagtga tcgacggcaa agtgcgggta
    19681 ggtgtggtac gggtcgagcg tgtagctctc gggcgccgca gtggcggcga ggggtaacgc
    19741 gagcgccacg gcgccgaggg taaggcgttg catgctgctt tcttctttag tgttgggggt
    19801 aacgtgggtc aaagcgtcca gccgcggctt tgcagccacg ccagcgcaca ggcgccagcg
    19861 gcgggcactg cgcagtaaac accggccagc accggttgca tccatagtgg tgaggcgggc
    19921 agcggcaggc gcgcggcgag cggcaccagc gccagcgccg ccagcgccac ccatggcacc
    19981 tgcgcgagca gcaccgatgc cgccgccagc agcgcgctca gcaccgacac ggtgagcgtc
    20041 agggcatggc cggcaccgac ccgcggcccg agggcaagga gcagcagcag gaatccgccg
    20101 caggcggcgc cgagcgccat gccgtactgg ccgatgagtg ccgaggcgcc gaacacggcg
    20161 ctcacgcccg cgcccagccc gagcatcagg ccggcggcgc cggcgcgcgc ggcatcgtgc
    20221 tgcagtgtca cggtcgcgga caccagccac accgtgagcg ccacgacgcc cgcgcccagc
    20281 agtgccaggt cgagcggcgc cttctgccgc agcacggtgg caaacaccca tagcgccgcc
    20341 ccgccgaaga tcgcgcccag cagcggcgcg ctgaagcgcg tcggcctgaa ggcaagatcg
    20401 tacagcgcgc ctacgagcgc cgcggcggcg ccggtgagca cgaccttgcg cggggcggta
    20461 agcggctcga gatcgaaatt gccgatagcg aagaccgcgg cgaggaagcc ggcgatggcg
    20521 gccaggccct cggcgcgcag ggggtaaagc agcgcagcgc acgccagccc ggccaggagc
    20581 ggcagcgcgc tcgactgcac gagcgggttg ccgagcgcgg tctgcagcag ggcgagcgcg
    20641 gcttccatct cagccccgcg ccacgcgcag cacgcccgag acgtcgcgca ccgcggcgct
    20701 cgcgcgcttg agctgcgcga ggtccgcgac ctcgaaggaa aagcgcatgg tggcgaggtc
    20761 gtcgcggctc tgcgtgcgca ccgcggtaac gttgatgtgc tcgcgcgcga gcgcatcgcc
    20821 gatgtcgcgc agcagccccg gccggtcgcg cgccgtcacc accatgtcga cgccgtagct
    20881 gcccgcggcc gcgccccact gcgcatcgag cagccgcgcg ctgtccgcct gcgccaggcg
    20941 cgcgaggctc gcgcagtcgg cgcggtggat cgacacgccg cgcccgcgcg tgacgaagcc
    21001 gcggatcgca tccggcggca ccggcttgca gcagcgcgcg atctgcgtca gcagccggtc
    21061 catgccgacc accaggatgc cgccgtcgcc cgccttcgcc ctgccctttt tcgccggcgc
    21121 ggtgggcgcc ggaagcggca ccagcttcgg ccgcggcggc ggctctggct cgcggccgcc
    21181 ctcctgcagg ttgaaccagt ggcgcacctt ggcacgtgcg cggctggtct tgaggaagcc
    21241 gcgctcggca ttgagccagt cgcgcgacgg cccgcccgtc ttggcgacgg tgatctccac
    21301 gcgctgcccg ctcttcagcg gcgtatcgag cggcacgatg cgcccgtcga ccttcgcgcc
    21361 gcggcagcgg tggccgatgt cggtgtgcag cgcgtaggcg aagtcgaccg gcgtcgcgcc
    21421 cgcgggcagg tccaccaccc ggccctgcgg cgtcaccacg tagatggtgt cgtcgagcgc
    21481 tgcggccgcc gtgcgctcgg cccaggtgcc gcggtccacc acctcgtcgc gccaggcgag
    21541 gagctggcgc agccacgcga tcttgtcgtc gtgcgcggtg tcggcgcgcg tcgtgcggcc
    21601 cgactccttg tagcgccagt gggcggcgac gccgagctcg gcgtggcggt gcatctcgcg
    21661 cgtgcggatc tgcacttcca gcgtcttgcc cccgggcccc aataccgccg tgtgcagcga
    21721 ctggtacagg ttctccttcg ggcgcgagac gtagtcgtcg aactcgcgct cgatcggctg
    21781 ccacaggttg tgcaccacac cgagcgccgc gtagcagtcc tgcacctcgg gcacgatgac
    21841 gcgcagcgcc cgcacatcgt gcagctgctc gaagtcgacg cccttcagcc gcatcttgtt
    21901 gcagatggag tagatgtgct tcgggcgccc gctcacctcg cccgccacgc ccgctgcggc
    21961 gagctcgcgc tctagcgtcg ccatggcctg cgcgatgtag cgctcgcgct cgacgcgccg
    22021 ctcatcgagc atgcgggcga tacgctggta ggtctcgggc tcgaggaagc ggaacgacag
    22081 gtcctcgagc tcccacttca gctgccacac ccccaagcgg ttggcgagcg gggcgtagat
    22141 gtcgagcgtc tcgcgcgcgt gggccgcacg ctcggcgctc gggtgcttgg cgaaccagcg
    22201 cagcgtctgc gtgcggctgg ccagccgtat cagcaccacg cgcacgtcct cgaccatgcc
    22261 gagcaccatc ttgcgcagcg cttcggactg ctgcgccgcg gtggcgcgcg tcagcacgcg
    22321 cagctggtag agcttctcgg cgcccgcggc gagcgccgcg atctcggcgc cgaatcgcgc
    22381 gccgagcgcg tcacccgcgg cgaggtactt gggcgcggcg aacagcaccc cggcggcgcg
    22441 cgccgccgga tcgacgccga tggcggcgag gctcgcggcg agcccgagcg cgtgcggcca
    22501 caccggctcc ccggtggaga gcagctgccc ctcgtaaagc ggcccggcga actccagcgc
    22561 ctgcgcgacg agcgcgcgtt cctcgggcgc caggccctcg gtgagcggtt cgagcggcgt
    22621 ggcgctcgtt tgcaggtgca actgcgccac ggagaccatg caggtattgt agcgggacca
    22681 tgccggacgt tcgatg
//
LOCUS       DBNHKE010000058        13266 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_168282, whole genome shotgun sequence.
ACCESSION   DBNHKE010000058 DBNHKE010000000
VERSION     DBNHKE010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_168282"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2696
                     /locus_tag="AC16O9_03675"
     CDS             <1..2696
                     /locus_tag="AC16O9_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR6515748.1"
                     /translation="GTLAVANAAPATLEEGGLPVVAARARVATQDLASARLDSLRIEL
                     AGGGLLEGVAELDAQGVHAKLEVRELDLQALRADLRRTALQGTLDIVLGKGAQSVRGA
                     LSQDGMRLEAQVERAGDVVVVQRFHATAASGEASGEGRITLGEPLLADARLRFARVDP
                     AAFGAYPQGSLSGTLTLDSRLGARPRVDAQWRLEDSALLGRALHSRGRAQFTRERVSR
                     AEAEAKFGASELTLKGSFGHGGDRLAWTLAIPDLGEIDPGLAGALRAEGTLVGALDAH
                     ELALSARSPTAHVEARLAGGIRSGGDWQGEILSLTNAGEYPLRMTGTTALRASRERVE
                     LGQFEAELEAGRLLVREASWAAGRLSTRGEFSALPARWLVLAAGAAAQVRTTLLLDGD
                     WSLAATPRLQGTVQLRRRSGDLTLLLAEEDFALELDQAALDARFAESGVRLQLDAASR
                     YGKLALSGDVGTAPGADGGLGLGAQSPLDLSARLDAAALSVLAQPYVTQARVDGRLWA
                     ELRASGTVAAPRLAGKLRGEGLRLDMPQYGVHLRDGGFAAELGADRLRLSQFSIQAGT
                     GTFSAEGEVPLRLAEGGARLAWSAREFGVLERPDLRLAVSGQGSAGFDGKRLSLQGEV
                     RADRGYLNIDQERLPRPGDDVVVLGRPRKPAAEQAALPLDLDVQLDLGQDLKLEGQNF
                     EGKLTGRVRCQTAEDGTLRAIGRVRMVNALVFAYGQRLQVDPGELIFNGAIDNPSLNV
                     TAWRRNQAVEAGVQVTGNVQTPRVQLVSNPPVPEAERLSWLVLGHAPGEASQADLGLL
                     QAAAGTLLAGGDALPLDRRVARAFGLDELTLRGRGEAEGNVVAFGKRFSDRLYISYEQ
                     GLGDAVGNLVKLDYALGRRWSLRAESGTTSGAGLFYRFSWD"
     gene            2721..3614
                     /locus_tag="AC16O9_03680"
     CDS             2721..3614
                     /locus_tag="AC16O9_03680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6515749.1"
                     /translation="MQGTPHWTDKNGIKLFLWRKAAAAGTPPRGTILFVHGSSMASQP
                     TFDLQVPGRPHSSVMEWFAARGFDTWCMDNEGYGRSDKSRAINFDIANGADDLAAGSG
                     YVLRTSGAKKLLLYGISSGALKAALFAQRHPERVARLALDAFVWTGEGSPTLAERRKK
                     LAEFQSKNRRPIDRAFVRSIFARDHPGTADEATIEAFADAILALDDSVPTGTYVDMCS
                     KLPLVDPAKITVPTLILRGQYDGIAGVDDLIEFFKRLPNPDKQFTVMAGISHASFQQK
                     NYLTVYHLLHAFFTQPEPVFL"
     gene            3611..4567
                     /locus_tag="AC16O9_03685"
     CDS             3611..4567
                     /locus_tag="AC16O9_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6515750.1"
                     /translation="MRRLAVALVALLWSLQAAAQAWPSKPVRIVVPLGAGGFADVPAR
                     ILAPKLGAQLGGSTFVENRPGAGSTIGADHVAKAEPDGHTLLFTATPHVISAWLYKSL
                     QYDALKSFVPVALIASGPYVLVVHPDLKVGSVAELVAAAKAQPGKIDYASSGNGSAQH
                     LVTALFATTAGIDLNHVPYKGSGQAMQDVLGGRVKVHFAGVPNIINHVRAGKLRALGV
                     TGTQRWPDMPDVPTIAEAGVAGYEATLWLCMLAPAGTPEPIVNRVSAEIGKALHDPEV
                     LQQLRNAGIAASFLGPQEFGAFMRAEHEKWGRVVKATGATIN"
     gene            complement(4578..4928)
                     /locus_tag="AC16O9_03690"
     CDS             complement(4578..4928)
                     /locus_tag="AC16O9_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515751.1"
                     /translation="MPGKAKYLFIASMDVAPEKEALFNEVYDQEHIPELLKVPGVLSV
                     RRAVTVPLTMSIGGEKKTIVAEGEPRYSAYYELESAEVLTSDAWAKAVEAGRWPSQVR
                     PHTRNRRHVLRKLT"
     regulatory      4996..5090
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5243..6322
                     /gene="gcvT"
                     /locus_tag="AC16O9_03695"
     CDS             5243..6322
                     /gene="gcvT"
                     /locus_tag="AC16O9_03695"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR6515752.1"
                     /translation="MRQTPLHPAHRAAGAKLVDFAGWEMPLHYGSQLEEHHAVRRDAG
                     MFDTSHMLAIDVEGTAAREFLRRALANDVARLTAPGKALYSCLLDEAGGVLDDLIAYF
                     LRDDLFRVIVNAGTAGKDLRWLESLRAGGVTLRSRRDLAMIAVQGPKAREKTWQALPG
                     LEAASAPLGAFYGAQAGDTFIARTGYTGEDGFELLLPAARATEAWQRLAAAGVRPCGL
                     GARDTLRLEAGMNLYGQDMDESVTPFEAGLGWSVVLDGARDFVGKAALQAKKPVRGLT
                     GLVLSGRGGVLRAHQKVHAANGAGEITSGGFSPTLERSIALARLPAGTAPGARVAVEV
                     RDKKLEARVVKPPFVRHGKSLIPES"
     gene            6336..6713
                     /gene="gcvH"
                     /locus_tag="AC16O9_03700"
     CDS             6336..6713
                     /gene="gcvH"
                     /locus_tag="AC16O9_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR6515753.1"
                     /translation="MNHPKDLKYTESHEWIRAENDGTLTIGITDHAQEALGDLVFIEL
                     PELGRKLAAEEACCVVESVKAASDVYAPVAGEVVAVNSAVVETPADLNKDPYAAWLIR
                     IKPAAKFDPAKLLDAATYEKGLA"
     gene            6715..9561
                     /gene="gcvP"
                     /locus_tag="AC16O9_03705"
     CDS             6715..9561
                     /gene="gcvP"
                     /locus_tag="AC16O9_03705"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR6515754.1"
                     /translation="MDFESLLHRDAFHARHIGPDADGEREMLALLGVATRAELVAQAM
                     PTAIRQREPLTLPGPLDEAEALDELRRITLQNRIWRSYIGTGYSACHTPQVIQRNVLE
                     NPGWYTAYTPYQAEISQGRLEVLLAFQQVVIDLTGLPVANASLLDEATAAGEAMTLMR
                     RASKSKAQAFFVDAACHPQVIAVVRTRAKWLGIPVVVGDAAQALDPAAVFGAHLQYPD
                     TYGAVRDWSDLIERVHAARGLVSLGTDLLALLLLKSPGALGADVAVGSAQRFGVPLGY
                     GGPHAGFMAVREALLRQMPGRIIGVSRDAAGRTAYRMALQTREQHIRREKATSNICTS
                     QVLLANIAALYAAWHGPEGLRRIALRCHALARLLAQAVAPESAAYFDTLSFAVGAAQR
                     EIRARAIEKQVNLRWIGAQRVGVSVDETTTPEDIADLAWVLTGRDGGIAERARALGAE
                     PAAIPPALRRTDAVLVHPVFHRYRSESEMMRYLKRLENRDYSLAQGMIPLGSCTMKLN
                     AAAEMAPLSWRHFAALHPFVPAGQAEGYARVLGDVAAALSAITGLPAMTMQPNSGANG
                     EYAGLVAIRNYHAARGEGARDVCLIPSSAHGTNPASAHLAGLKVVVVDCDAQGNVDLA
                     DLDARLAQHAARLAALMVTYPSTHGVFEADIKKICERVHAAGGQVYMDGANLNALAGL
                     ARPADLGADVCHINLHKTFCIPHGGGGPGMGPIGVAAHLAPHLPTHPVVPAPGCDAEN
                     GTVSASPHGSPLIVTISWMYLRMMGAAGIRRASETAILSANYIAARLAPYFPVLYSGE
                     RGRVAHECILDLRDLKDAYGVNAEDVAKRLMDYGFHAPTLSFPVADTLMVEPTESEAK
                     GELDRFCDAMIAIHGELQRIKRGEWDAKDNPLHNAPHTAEELAGDWPHPYSREVAAYP
                     LPALRAHKYWPPVKRLDQVYGDRNFSCTLPPDER"
     gene            9558..10757
                     /locus_tag="AC16O9_03710"
     CDS             9558..10757
                     /locus_tag="AC16O9_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6515755.1"
                     /translation="MKITAIETLRLDEFPNLLWVQLHTDQGLVGLGESFYGPDSAEGH
                     IHGIIAAYLLGQDPLLIDRHHANLIGYVGFSGSSAEQRGRSAVDIALWDLWGQSCGQP
                     VHQLLGGAVRDDIRVYNTCAGYRYVRAKPVQGTANFGLGQAEGPYEDLDAFLTRADEL
                     ARSLLDMGITGMKIWPFDFAAEASSGAYLSAPDLDKALEPFAKIRKAVGNKMDVMCEL
                     HALWNLPAAKGIVRALEPYAPLWIEDPVFMDQLESLGELQAGTHQMIATGETLGGRAQ
                     YKDLLQRRGAGMAIMDIAWCGGISEARKIAAMAEAWHASVAFHDCTGPVVLAASTHLA
                     MNARNCFIQEIVRAFYYGWYGELVSALPPIRNGRISAPPGPGLGLKLNAERFKASDVR
                     RRTSRAS"
     gene            complement(10825..11310)
                     /locus_tag="AC16O9_03715"
     CDS             complement(10825..11310)
                     /locus_tag="AC16O9_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR6515756.1"
                     /translation="MASLTELGAAVGARLRERGQTLAVAESSAGGLINAALVAVPGAS
                     AYYIGGCIIYTAAGREALLGISAQDMAGMRSASEPFARLLSRRVREKLGTTWGLAETG
                     ASGPSGNRYGDPAGHACIAVAGPIEAVITLATGSAAREDNMWLFARRAVALLDECVNK
                     T"
     gene            11343..11870
                     /gene="ppa"
                     /locus_tag="AC16O9_03720"
     CDS             11343..11870
                     /gene="ppa"
                     /locus_tag="AC16O9_03720"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR6515757.1"
                     /translation="MRLEAIRVGHNPPEDINVIVEVPVGGEPIKYEIDKASGALVVDR
                     FLYTPMRYPGNYGFVPHTLCGDGDPLDVLVASTRALVPGSVINCRPVGVLVMEDESGM
                     DEKLIAVPVSKLTRRYDGVKTLADLPEITRSQIEHFFQHYKDLEPGKWVKIVRWGDAA
                     SAREIIVDAIARARR"
     gene            complement(11867..13198)
                     /locus_tag="AC16O9_03725"
     CDS             complement(11867..13198)
                     /locus_tag="AC16O9_03725"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6515758.1"
                     /translation="MTRRPALPVLVLALWAPLALAQSLALETVAVGLTAPIHLEEPAD
                     GSGRKFIVQQDGVVRVLGADGRLAPEPFLDLRARLLPLERGFEERGLLGFALHPQFAR
                     NGRVYASYSSPLRAGAPPGWNHTRRVSEFTARPGELSRVDADSERVLLELDWPSRKHN
                     GGGLAFGPDGYLYIGLGDSGASHGIGKEVRWEAFNVPAEALAWDNLAQDRESLFGKIL
                     RIDVDRGFPGYAVPRGNPFAAGGGRGEIWAWGFRNPFRIAFDRDGSGDFYVTAVAETL
                     WEAAYRVQRPGNFGWPLIEGAHCVDRLRPRQPPASCAQRDAFGTPLELPVVEYPNMQA
                     SHPETKLGVAGVGTAITGARRYRGRALPSLAGQLVVTDWSASFKQPSGQIFVATPAAD
                     GSLWPMRRVLQLDTRIVGLAEDRAGELYVLTNETFGPYGDSGKVLRLVERR"
BASE COUNT         2001 a         4762 c         4617 g         1886 t
ORIGIN      
        1 ccggtacgct cgccgtcgcc aatgccgcgc ccgccacgct cgaggagggc ggcttgcccg
       61 tcgtggccgc gcgagcacgc gtggccacgc aggatctggc gagcgcgcgg ctcgatagcc
      121 tgcgcatcga gctcgccggt ggcggcctgc tcgaaggcgt ggccgagctc gacgcgcagg
      181 gcgtgcacgc caagctcgag gtccgcgagc tcgacctgca ggcgctgcgc gcagacctgc
      241 ggcgcacggc gctccagggc acgctcgaca tcgttctcgg caagggtgcg cagtcggtgc
      301 gcggcgcgct gtcgcaggac ggcatgcgcc tcgaagccca ggtggagcgc gcgggtgacg
      361 tcgtcgtcgt gcagcgcttt catgcgacgg cggcgagcgg cgaagcaagt ggcgaagggc
      421 gcatcacgct gggcgagccg ctcctcgccg atgcgcggct gcgctttgcg cgcgtcgatc
      481 ccgccgcctt cggtgcgtac ccgcagggat cgctgagcgg cacgctgacg ctcgacagcc
      541 gtctcggcgc ccggccgcgc gtcgacgccc aatggcggct cgaggacagc gcgctcctcg
      601 gtcgggcact gcacagccgc ggtcgcgcgc agttcacccg cgaacgcgtg tcgcgcgccg
      661 aagccgaggc gaaattcggt gcgagcgagc tgacgctgaa gggaagcttt ggccacggcg
      721 gcgaccggct cgcctggaca ctcgcgatcc cggatctggg cgagatcgat cccgggctgg
      781 ctggcgcgct gcgcgccgag ggcacgctcg tcggcgccct ggacgcgcac gagctcgcgc
      841 tcagcgcgcg ttcgccgacc gcccacgtcg aggcgcgtct cgcgggcggc atccggtccg
      901 gcggcgactg gcagggcgag attctctcgc tcaccaatgc gggcgagtac cccttgcgca
      961 tgaccggcac gacggcgctg cgcgcctcac gagagcgcgt cgagctcggg cagttcgagg
     1021 ccgagctgga ggcggggcgg ctcctggtgc gcgaggcctc gtgggccgcg gggcggctgt
     1081 cgacgcgcgg cgaattcagc gccctgccgg cgcgctggct ggtgctcgcc gccggcgcgg
     1141 cggcgcaagt gcgcaccacg ctgctcctcg atggcgactg gtcgctcgcc gcaacccccc
     1201 ggctgcaggg tacggtgcag ctcaggcgcc gcagcggcga cctgacgctg ctcctggcgg
     1261 aggaggattt cgccctcgag ctcgaccagg ccgcgctcga cgcccgtttt gccgagagcg
     1321 gcgtgcgcct gcagctcgac gcggcctccc gctacggcaa gctcgcgctc tcgggcgatg
     1381 tggggaccgc gcccggggcg gacggcggac tcgggttggg cgcgcaatcg ccgctcgact
     1441 tgagcgcgcg gctcgacgcc gcggcgttgt cagtcctcgc gcagccctac gtgacgcagg
     1501 cgcgcgtgga cgggcgcctg tgggccgagc tgcgcgcctc cggaacggtg gccgcgccgc
     1561 gcctcgccgg caagctgcgc ggcgaggggc tgcggctcga catgccccag tacggcgtgc
     1621 atctgcgcga cggcggcttc gccgccgagc tcggcgccga tcggctgcgg ctctcgcagt
     1681 tctcgattca ggcgggcacg ggcacgttca gcgccgaggg cgaagtgccg ctgcgcctgg
     1741 ccgagggcgg cgcccggctc gcctggtcgg cgcgcgagtt cggcgtgctt gagcggcccg
     1801 acttgcgcct cgcggtctcc ggccagggca gcgccgggtt cgacggcaag cggctgtcgc
     1861 tccagggcga ggtgcgcgcc gaccggggct atctgaatat cgaccaggaa cgcctgccgc
     1921 ggccgggcga cgatgtcgtg gtcctgggcc ggccgcgcaa gcccgccgcc gagcaagccg
     1981 cgctgccgct cgacctggat gtccagctcg accttggcca agacctgaag ctcgagggcc
     2041 agaacttcga gggcaagctc accggccgcg tgcgctgcca gacggcggaa gacggcaccc
     2101 tgcgcgcgat cgggcgcgta cgcatggtca atgccctggt ctttgcctac ggccagcgcc
     2161 tgcaggtcga tcccggcgag ctgatcttca acggcgcaat cgacaacccg tcgctcaacg
     2221 tcaccgcctg gcgccgcaac caggcggtcg aggccggcgt gcaggtcacc ggcaacgtgc
     2281 aaacgccgcg cgtgcagctc gtctccaacc cgccggtgcc cgaggcagag cgcctgtcgt
     2341 ggctcgtgct cgggcacgcg ccgggcgagg cgagccaggc cgacctcggg ctgctgcagg
     2401 cggcagcggg gaccctgctc gcgggcggcg acgccctgcc gctcgaccgg cgcgtggcgc
     2461 gtgccttcgg cctggatgag ctgacgttgc gtggccgcgg cgaggccgaa ggcaacgtag
     2521 tggcgttcgg caagcggttt tccgaccggc tctacatcag ctacgagcaa ggactggggg
     2581 acgcggtggg caacctcgtc aagctggact acgcgctcgg caggcgctgg tcgctgcgcg
     2641 ccgagagcgg cacgacgagt ggcgcgggac tcttctaccg cttctcctgg gactgagccg
     2701 gcaaggtaaa ctcgcgcgcc atgcaaggca ccccgcactg gaccgacaag aacggcatca
     2761 agctttttct gtggcgcaag gccgctgcag ccggcacgcc gccgcgcggc acgatcctct
     2821 tcgtgcacgg ctcctcgatg gcctcgcaac ccactttcga cctgcaggtg ccggggcggc
     2881 cgcattcctc ggtcatggag tggttcgcgg cgcgcggctt cgacacctgg tgcatggaca
     2941 acgaaggcta cgggcgctcc gacaagtcgc gcgcgatcaa cttcgacatc gccaacggcg
     3001 ccgacgatct cgctgccggg agcggctacg tgctgcgcac gagcggcgcg aagaagctgc
     3061 tgctgtacgg catcagctcg ggcgcgctga aagccgcgct gttcgcccag cgccatcccg
     3121 agcgcgtggc gcgcctcgcg ctcgatgcgt tcgtctggac cggcgagggc agcccgacgc
     3181 tcgccgagcg ccgcaagaag ctcgccgagt tccagtcgaa gaaccgccgg ccgatcgacc
     3241 gcgcgttcgt gcgcagcatc ttcgcgcgcg accatcccgg caccgcggac gaggcgacca
     3301 tcgaggcgtt cgccgacgcc atcctcgcgc tggacgactc ggtgcccacc ggcacctacg
     3361 tcgacatgtg ctcgaagctg ccgctcgtcg acccggcgaa gatcaccgtg ccgaccctca
     3421 ttctgcgcgg gcagtacgac ggcatcgccg gggtggacga tctgatcgag tttttcaagc
     3481 gcctgccgaa cccggacaag cagttcacgg tgatggccgg catctcgcac gcgagtttcc
     3541 agcagaagaa ctacctgacc gtgtaccacc tcctgcacgc cttcttcacc caacccgaac
     3601 cggtgttcct atgagacgcc ttgccgtcgc actcgttgcc ctcctctggt cgctccaggc
     3661 tgccgcgcag gcctggccct cgaagcccgt gcgcatcgtg gtgccgctcg gcgccggcgg
     3721 cttcgccgac gtgccggcgc gcatcctcgc gccgaagctg ggcgcgcagc tcggtggcag
     3781 caccttcgtg gaaaaccgtc ccggcgcggg cagcaccatc ggcgccgacc acgtcgccaa
     3841 ggccgagccc gacggccaca cgctgctctt taccgccacg ccgcacgtca tcagcgcctg
     3901 gctctacaag tctttgcagt acgacgcgct gaagtccttc gtacccgtgg cgctcatcgc
     3961 ctcggggccc tacgtgctgg tggtgcatcc ggacctgaag gtgggctcgg tggccgagct
     4021 cgtcgctgcg gccaaagcac agccgggcaa gatcgactac gcgagctccg gcaacggcag
     4081 cgcgcagcac ctggtgacag cgcttttcgc caccacggcc ggcatcgacc tcaaccatgt
     4141 gccgtacaag ggcagcggcc aggcgatgca ggacgtgctt ggtggacgcg tcaaggtgca
     4201 cttcgcgggc gtgccgaaca tcatcaacca cgtgcgcgcg ggcaagctgc gcgcgctcgg
     4261 cgtgaccggg acgcagcgct ggcccgacat gcccgacgtg ccgacgatcg cggaggcagg
     4321 cgtcgcgggc tacgaggcga cgctgtggct gtgcatgctc gcgcctgccg gcacgccgga
     4381 gccgatcgtg aaccgggtat cggccgagat cggcaaggcg ctgcacgacc cggaagtgct
     4441 gcagcagctg cgcaacgccg gcatcgccgc gagcttcctc ggcccgcagg agtttggcgc
     4501 cttcatgcgc gccgagcacg agaagtgggg ccgcgtggtg aaagccaccg gcgccacgat
     4561 caactagccg gcggcgtcta agtcagcttg cgcagcacgt gccgccggtt gcgcgtgtgc
     4621 gggcgcacct gcgacggcca gcgcccggct tccaccgcct tcgcccaggc gtcgctcgtc
     4681 agcacttcgg cgctctccag ctcgtagtaa gcgctgtagc gcggctcgcc ctcggcgacg
     4741 atggtctttt tctcgccgcc gatggacatg gtgagcggca cggtcacggc gcggcgcacc
     4801 gagagcacgc ccggaacctt gagcagctcg ggtatgtgct cctggtcgta gacctcgttg
     4861 aacaacgcct ctttttcggg cgccacatcc atgctggcaa tgaacaggta cttggctttg
     4921 cccggcatct gggtcctccg tggggttcgt ctcgccctgc agtctaaccg tcccgggtga
     4981 cagtgataaa ctgagtctcg ctgcggggaa gagagtggca caagcagcaa ggccgccgcc
     5041 gaaggcgtaa gcgcccgcaa tcgctcaggc aaaagaactc cgcagcggca actactctgg
     5101 agagcggcga gccgaactcg cccaccgaag gggccagcgg cgcagcgcgg agcgcgcgcc
     5161 gtaaactctc aggtcaaagg acagaggggc agcgccggac gaccggccct gccccgtatt
     5221 gctgtccgga gggaccagcc gcttgcgcca gaccccgctg caccccgcgc accgcgccgc
     5281 cggcgccaag ctggtcgatt tcgccggctg ggagatgccg ctgcactacg gctcgcagct
     5341 cgaggagcac catgcggtgc gtcgcgacgc gggcatgttc gacacctcgc acatgctcgc
     5401 gatcgacgtc gaaggcaccg cggcgcgcga gttcctgcgc cgcgcgctcg ccaacgacgt
     5461 cgcgcgcctg acggcgcccg gcaaggcgct ctactcttgc ctgctggacg aggcgggtgg
     5521 cgtgctcgac gacctcatcg cgtatttcct gcgcgacgac ctgttccgcg tcatcgtcaa
     5581 tgccggcacc gccggcaagg acctgcgctg gctcgaaagc ctgcgcgccg gcggcgtcac
     5641 gctgcgctcg cggcgcgacc tcgccatgat cgcggtgcag ggacccaagg cacgtgaaaa
     5701 gacctggcag gcgttgcctg ggctcgaggc cgcgagcgcg ccgctcggcg cgttctatgg
     5761 cgcgcaggcg ggcgacacct tcatcgcgcg caccggctac accggcgagg acggattcga
     5821 gctgctgctg cccgcggcac gcgccaccga ggcctggcag cgcctcgccg ccgcgggcgt
     5881 gcgcccttgc ggcctcggcg cgcgcgacac gctgcgcctc gaggcgggca tgaaccttta
     5941 cggccaggac atggacgagt cggtgacgcc gttcgaagcg ggccttggct ggagcgtcgt
     6001 gctcgacggc gcgcgcgact tcgtcggcaa ggcggcgctg caggcgaaaa agcccgtccg
     6061 cggcctgacc ggcctggtcc tctccggccg cggcggcgtg ctgcgcgcgc accagaaagt
     6121 gcatgccgcg aacggcgcgg gcgagatcac gagcggcggc ttctcgccga cactggaaag
     6181 atcgattgcg ctcgcgcgcc tgcccgccgg caccgcgccc ggcgcgcgcg tcgcggtcga
     6241 ggtgcgcgac aagaagctcg aggcgcgcgt ggtcaagccg ccgttcgtgc gccacggcaa
     6301 gtccctcatc cccgaatcct gatgcggagc accccatgaa ccaccccaag gacctcaaat
     6361 acaccgaatc gcacgagtgg attcgcgcgg agaacgacgg cacattgacg atcggcatca
     6421 ccgaccatgc gcaggaagcg ctcggcgacc tggtgttcat cgagctgccc gaactcgggc
     6481 gcaagctcgc ggccgaggag gcctgctgcg tggtcgagtc ggtcaaggcg gcctccgacg
     6541 tctacgcgcc ggtggcgggc gaagtggtgg cggtgaacag cgccgtggtc gagacgccgg
     6601 ccgatctgaa caaggatccc tacgcggcgt ggctcatccg catcaagccg gcggcgaaat
     6661 tcgatcctgc caagctgctc gacgccgcga cctacgagaa aggccttgcc tgacatggac
     6721 ttcgagtcgc tcctgcaccg cgacgccttc cacgcgcgcc acatcgggcc cgatgcggac
     6781 ggcgagcgcg agatgctcgc ccttctgggg gtcgccacgc gcgccgagct ggtcgcgcag
     6841 gcgatgccca ccgccatccg ccagcgcgag ccgctcacgc tgcccgggcc gctcgatgaa
     6901 gccgaggcgc tcgatgagct gcgccggatc acgttgcaga accgcatctg gcgcagctac
     6961 atcggcaccg gttattcggc ctgccatacg ccgcaggtga ttcagcggaa cgtcctcgag
     7021 aaccctggct ggtacacggc gtatacgccg taccaggccg agatctcgca aggccggctc
     7081 gaagtgctgc tcgccttcca gcaggtggtg atcgacctca ccggcctgcc ggtggcgaac
     7141 gcctcgctgc tcgacgaagc gaccgccgcg ggcgaggcga tgacgctgat gcggcgcgcc
     7201 tccaaatcga aagcccaggc gttcttcgtc gacgccgcct gccacccgca ggtgatcgcg
     7261 gtggtccgca cgcgcgccaa atggctcggc atcccggtgg tggtcggcga cgcggcgcag
     7321 gcgctcgatc cggcggcggt gttcggcgcg catctgcagt acccggatac ctacggcgcg
     7381 gtgcgcgact ggtcagatct aatcgagcgc gtgcacgccg cgcgggggct ggtatcgctc
     7441 ggcaccgacc tgctcgcgct gctgcttctg aagtcgcccg gcgcgctcgg cgccgacgtc
     7501 gccgtcggct cggcgcaacg tttcggcgtg ccgctcggct acggcgggcc gcacgccggc
     7561 ttcatggcgg tgcgcgaggc gctcctgcgg cagatgcccg ggcgcatcat cggcgtgagc
     7621 agggacgccg cgggacgcac cgcctatcgc atggcgctgc agacgcgtga gcagcacatc
     7681 cgccgcgaaa aagcgacgag caatatctgc acctcgcagg tgctgctcgc caacatcgcc
     7741 gcgctgtacg ccgcgtggca cgggcccgag ggactgcggc gcatcgcgct gcgctgccac
     7801 gcgctcgcgc ggctgctcgc gcaggcggtg gcgcccgagt ccgccgcgta cttcgatacg
     7861 ctgagcttcg cggtgggcgc cgcgcagcgc gagatccgcg cgcgcgcgat cgagaagcag
     7921 gtcaacctgc gctggatcgg cgcgcagcga gtcggcgtga gcgtggacga gaccacgacg
     7981 cccgaggaca tcgccgatct cgcctgggtg ctgaccggca gggacggggg cattgccgag
     8041 cgcgcccggg cgctgggcgc ggaacccgcg gcgattccgc cggcgttgcg gcgcactgat
     8101 gcggtgctcg tgcacccggt gttccatcgc tatcgcagcg agagcgaaat gatgcgctat
     8161 ctcaagcgcc tcgagaaccg cgactactcg ctcgcgcagg gcatgatccc gcttggctcc
     8221 tgcaccatga agctgaacgc ggccgcggaa atggcgccgc tgtcatggcg gcacttcgcc
     8281 gcgctgcatc cgttcgtgcc cgccgggcag gcagaaggct atgcgcgcgt gctgggcgac
     8341 gtcgccgccg ccctgagcgc gatcacgggc ctgccggcga tgacgatgca gccgaactcg
     8401 ggcgccaacg gcgaatacgc ggggctggtc gcgatccgca actaccacgc cgcgcgcggc
     8461 gagggagcgc gcgacgtgtg cctgattccc tcctcggcgc acggcaccaa tccggcctcg
     8521 gcgcacctcg ccgggctcaa ggtggtggtg gtcgactgcg acgcgcaagg caacgtcgat
     8581 ctggccgatc tcgacgcgag gctcgcgcag cacgcggcgc ggctcgccgc gctgatggtc
     8641 acctacccct cgacgcacgg cgtgttcgaa gccgacatca agaagatctg cgagcgcgtg
     8701 cacgccgcgg gcggccaggt gtacatggac ggcgcgaacc tgaatgcgct cgccggcctc
     8761 gcgcgtcccg ccgacctcgg cgcggacgtc tgccacatca acctgcacaa gaccttctgc
     8821 atcccgcacg gcggcggcgg ccccggcatg gggccgatcg gcgtcgccgc gcacctggcg
     8881 ccgcacctgc cgacgcatcc ggtcgtgccc gcgcccggat gcgacgcgga gaacggcacg
     8941 gtgagcgcct cgccgcacgg cagcccgctg atcgtcacca tctcgtggat gtacctgcgc
     9001 atgatgggcg ccgcgggcat ccgccgcgcg agcgaaacgg cgatcctgtc ggcgaactac
     9061 attgctgcac ggctcgcgcc gtatttcccc gtgctgtact cgggcgagcg cgggcgcgtc
     9121 gcgcacgagt gcatcctcga cctgcgcgac ctgaaagacg cgtacggcgt gaacgccgag
     9181 gacgtcgcca agcgcctcat ggattacggc tttcacgcgc cgacgctctc tttcccggtc
     9241 gccgacacgc tgatggtcga gcccaccgaa agcgaggcca agggcgagct cgaccgcttc
     9301 tgcgacgcga tgatcgcgat ccacggcgag ctgcagcgca tcaagcgcgg cgagtgggac
     9361 gcgaaggaca atccgctgca caatgcgccg cacaccgccg aggagctggc gggcgactgg
     9421 ccgcatccgt actcgcgcga ggtggccgcc tacccgctgc ccgcgcttcg cgcgcacaag
     9481 tactggccgc cggtgaagcg cctcgaccag gtgtacggcg atcgcaactt ctcctgtacc
     9541 ctgccgccgg acgagcgatg aagatcaccg ccatcgaaac cctgcgcctc gacgagttcc
     9601 cgaacttgct ctgggtgcag ctgcacaccg accagggcct ggtcggcctg ggcgaatcct
     9661 tttacggccc cgactcggcc gagggccaca tccacggcat catcgctgcg tacctgctgg
     9721 ggcaggaccc gctgctgatc gaccggcacc acgccaacct cataggctac gtcggcttca
     9781 gcggctcgag cgccgagcag cgcgggcgct cggccgtcga catcgcgctg tgggatctgt
     9841 ggggacagtc ctgcggccag cccgtccacc agctcctggg cggcgcggtc cgcgacgaca
     9901 tccgcgtcta caacacctgc gccgggtacc gctacgtgcg cgcgaagccc gtgcagggca
     9961 ccgcgaactt cgggctcggc caggccgaag gcccgtacga ggatctcgat gccttcctta
    10021 cccgcgccga cgagctggcg cgaagcctgc tcgacatggg catcacgggg atgaagatct
    10081 ggccgttcga cttcgccgcc gaagcgtcaa gcggcgccta cctctccgcg cccgacctgg
    10141 acaaggccct cgagcccttc gcgaagatcc gcaaagccgt cggcaacaag atggacgtaa
    10201 tgtgcgaatt gcacgcgctg tggaacctgc ccgccgccaa gggcatcgtg cgcgcgctgg
    10261 agccttacgc gccgctatgg atcgaagacc cggtgttcat ggaccagctg gaaagcctcg
    10321 gcgagctgca ggccggcacg caccagatga tcgccacggg cgagacgctc ggcgggcgcg
    10381 cgcagtacaa ggacctcctg cagcgccgcg gcgccggcat ggcgatcatg gacatcgcct
    10441 ggtgcggcgg catctcggaa gcgcgcaaga tcgcggcgat ggcggaggcc tggcacgcga
    10501 gcgttgcctt ccacgactgc accggacccg tggtgctcgc cgcgtcgacg cacctggcga
    10561 tgaacgcgcg caactgcttc atccaggaga tcgtgcgcgc gttctactac ggctggtacg
    10621 gcgagctggt cagcgcgctg ccgccgatca ggaatgggcg catcagcgcg ccgcccgggc
    10681 ccgggctggg actgaagctc aacgccgagc gcttcaaggc cagcgacgtt cgccgccgca
    10741 ccagccgcgc gtcatagtcc cagcgccgcc ctctgatgat cgcagccttg ccttcaagcg
    10801 tgcccacggc tcacgtcctt tcgttcaggt cttgttcacg cattcgtcga gcaacgccac
    10861 cgcgcgccgc gcaaaaagcc acatgttgtc ctcgcgtgcc gcgctgcccg tagcgagcgt
    10921 gatgaccgcc tcgatcggtc cggcgaccgc gatacaggcg tggcccgcgg gatcgccgta
    10981 acgatttccg gatgggccgc tcgcgccggt ctcggcgagg ccccaggtgg tgccgagctt
    11041 ctcgcgcacc cgccgcgaca gcagccgcgc gaatggctcg ctcgccgagc gcatgcccgc
    11101 catgtcctgc gcggaaatgc cgagcagcgc ctcacgcccg gccgccgtgt aaatgatgca
    11161 gccgccgatg tagtaggccg aggcgccagg cacggccacc agcgccgcgt tgatcaggcc
    11221 gccggccgag gattcggcca ccgccaacgt ctgtccgcgt tccctgagtc gcgcgcccac
    11281 cgcggcgccg agctccgtca acgatgccat ccgcgcctcc ccgccgttac acttgggccc
    11341 tcatgcgcct ggaagccatc cgcgtcggcc acaatccccc ggaggacatc aacgtcatcg
    11401 tcgaggtccc ggtcggcggc gagccgatca agtacgagat cgacaaggcg tccggcgcgc
    11461 tggtggtcga ccgcttcctt tacaccccga tgcgctatcc gggcaactac ggtttcgtgc
    11521 cgcacacctt gtgcggcgac ggcgacccgc tggacgtgct cgtcgccagc acgcgcgcgc
    11581 tggtgccggg ttcggtgatc aactgccggc cggtgggcgt gctggtgatg gaggacgaat
    11641 cgggcatgga cgagaagctg atcgccgttc cggtgagcaa gctcacgcgg cgctacgatg
    11701 gcgtgaagac gctcgccgac ctgcccgaga tcacgcgcag ccagatcgag catttcttcc
    11761 agcattacaa ggatctcgag ccgggcaagt gggtgaagat cgtgcgctgg ggcgacgcgg
    11821 cgagcgcgcg cgagatcatc gtcgacgcca tcgcccgcgc caggcgctag cggcgctcga
    11881 ccagacgcag caccttgccg ctgtcgccgt acggtccgaa tgtctcgttg gtgagcacgt
    11941 agagctcccc ggcacggtcc tcggcgaggc cgacgatgcg tgtgtcgagc tgcagcacgc
    12001 ggcgcatcgg ccacagcgac ccgtccgcag ccggcgtcgc cacgaagatc tgcccggacg
    12061 gctgcttgaa cgaagcgctc cagtcggtca cgacgagctg gccggcgagg ctgggcaggg
    12121 cgcgcccccg gtacctgcgc gcgccggtga tcgccgtgcc gacgcccgcg acgccgagct
    12181 tcgtctcggg atgcgaggcc tgcatgttgg gatactccac gaccgggagc tcgagcggcg
    12241 tgccgaacgc gtcgcgctgc gcgcagctcg ccggcggctg gcgcggccgc agccgatcga
    12301 cgcagtgcgc gccctctatc aggggccagc cgaagttgcc cggccgctgc acgcggtatg
    12361 ccgcctccca cagcgtctcg gcgaccgcgg tgacgtagaa gtcgccgctg ccgtcgcggt
    12421 cgaaggcgat gcgaaacgga ttgcgaaagc cccaggccca gatctcgccg cgccccccgc
    12481 cggccgcaaa cgggttgccg cggggcacgg cgtagcccgg gaagccccgg tcgacgtcga
    12541 tgcgcaggat cttgccgaag agcgactcgc ggtcctgcgc gaggttgtcc caggcgagcg
    12601 cctcggccgg cacgttgaac gcttcccagc gcacttcctt gccgatgccg tgcgaggcgc
    12661 cgctgtcgcc gagcccgatg tacagatagc cgtccgggcc gaaggcgagc ccgccgccgt
    12721 tgtgcttgcg gctcggccag tccagttcca gcagcacgcg ctcggagtcg gcgtcgaccc
    12781 ggctgagctc gccgggcctc gccgtgaact cggaaacgcg ccgcgtgtgg ttccaccccg
    12841 gcggcgcccc ggcgcgcagc ggcgacgagt agctcgcgta cacgcgcccg ttgcgcgcga
    12901 actgcggatg gagcgcgaag ccgagcaggc cgcgctcctc gaagccgcgc tcgagcggca
    12961 gcaggcgcgc gcgcaagtcc agaaatggct cgggggcgag cctgccgtcg gcgccgagca
    13021 cgcgcaccac gccgtcctgc tgcacgatga acttgcgccc gctgccgtcc gcgggctctt
    13081 ccagatgaat cggcgcggtg aggccgaccg caaccgtctc cagcgccagc gactgcgcga
    13141 gcgccaacgg cgcccacagc gcgagcacga ggaccggaag cgcgggccgg cgggtcattg
    13201 gcacgtagtc taattgctcc cggcgcggcc gcgtaccgac cacagcgaca gcgcttcacg
    13261 cttgcc
//
LOCUS       DBNHKE010000059        14342 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_486508, whole genome shotgun sequence.
ACCESSION   DBNHKE010000059 DBNHKE010000000
VERSION     DBNHKE010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_486508"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1503
                     /locus_tag="AC16O9_03730"
     CDS             103..1503
                     /locus_tag="AC16O9_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR6515759.1"
                     /translation="MQTRKIVILLAALAMSAMGAIVGAQYLNTGVAQAAAPVAAAQLP
                     DFAGIVQRYGPAVVNIATSGTRKAGAQQAAPELFRGLPFQFGTPMPEMPVQGQGSGFI
                     LSTDGIVLTNAHVLQNATEVTVKLTDRREFKAEVLGTDPATDIAVLRIDARELPVVRL
                     GDPKRARVGDWVVAIGSPFGFENSVTAGIVSAKGRSLPGDIYVPFIQTDVAVNPGNSG
                     GPLFNLDGEVIGINSQIYSRSGGYQGLSFAIPIDMALRVKDQIVATGHATHARLGVNV
                     QTMNQALAESFGLERPQGALVAAVAPDSPAQHAGLQPGDVVVKFNGRPVGDSGELAAM
                     VGESTPGAKVQFEVLRKGERRTLAATLGTAEDKSKQQAADDEAAGKGRLGLAVRPLTP
                     QELEAAHVPGGLLVQGVNGAAARAGIAPGDIVLQADGKPVASVEDLRSATQSGKTIAL
                     LVQRGERRLFVPLQAG"
     gene            complement(1510..2715)
                     /locus_tag="AC16O9_03735"
     CDS             complement(1510..2715)
                     /locus_tag="AC16O9_03735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515760.1"
                     /translation="MRSRPDPLLFAVCALGITQITAWGTSYYCLGVLANPIARDTGWS
                     LSLIYFGFTVSLLVMGAVSTWAGRAIDRFGAQAVMSTGTVLVSAGLLLLSLVTSEAAY
                     LGVWAFLGVGMRLCLYDAAFAALVQVVPSRGRRAISYLTLFGAFASTVFWVVGHYLNE
                     ALGWRETLGWFALINLSVCLPLNWFGLARREPAGGHEAAAAKPSVDGAPLQGKVRTIA
                     IALFALVMSLNGFVFGVVTVQLVPLLEAAGLATAAAVWVASMKGFAQFGGRLVEIVFG
                     RNLRAITVARIAIGVLPASFAVLLFSGGNLQAVIAFTLLMGASQGVITIVRGAVPLAL
                     FGATGYGAVLGVLATPILIVNAASPTIFALIVDGWGWNAAEVVLLAAAAASWLAMEAM
                     SRWYEKRRR"
     gene            2789..3766
                     /locus_tag="AC16O9_03740"
     CDS             2789..3766
                     /locus_tag="AC16O9_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515761.1"
                     /translation="MATRRSGLKRAPAKRRVKKAQALSRGLTPAECRLDMLSGEAEEV
                     RERVKQAGGEVIGTYKDPLGGGALLSCILPISEVEPTPFQRDLSDAHHKKLADVIDRT
                     GMFLDPIICVTAPKDGFWTPNGMHRLMALRRLGAKSVTALVVPKREVAWQILALNTEK
                     AHNLKEKSLEVIRIYRGLIEEDAARPEAGFSFYLEEASFVTMGLCYEKNAKFAGGAYN
                     PVVRRLEKFSDEPLSKALKSHEKHAAMLQELDENVAEVIKKLKARGFQSPYLRAFVVA
                     RVNPLRFMQGEPPALEDVLKTMIGRAAKFNTEKVKQEDLASAGGAPDEA"
     gene            complement(3763..5388)
                     /locus_tag="AC16O9_03745"
     CDS             complement(3763..5388)
                     /locus_tag="AC16O9_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR6515762.1"
                     /translation="MSKPQPPSTSNFIKQVIEADLASGKHKRLVTRFPPEPNGYLHFG
                     HAKSIVLNFGLAQEYGGRCNLRFDDTNPEKEEQAYVDSIEDMVRWLGYDYGKALYASD
                     YFDFMYRCAEHLAQQGLAYVDSQSAEQMQHKRGSFTEPGTDSPYRSRSPGENLALLRE
                     MRDKKHPDGTHVLRAKIDMASPNINLRDPVLYRIKHVAHHRTGDNWCIYPTYTYAHPI
                     EDALEGITHSLCTLEFEDQRPFYDWLLGKLADGGLLKRPLPQQIEFARLNLTHVVLSK
                     RRLVELVDKGHVDGWDDPRLPTLAGARRRGYTPEGFRRFAERIGISKANQVIDFSVLE
                     DAMREHLNEVAPRRMAVLDPVKLVIENYPAGIEETVEVPNHPQKPQMGKRSVPFSGEL
                     WIEREDFQEAPPKGYFRLYPGNQVRLRYGYVVKCIGYDKATDTVRCTYLQDSRSGTPG
                     ADKYKVKGNIHWVSAKHAHAASVHLYDRLFADLQSDGLNPGSKKTIGAKLEPCLQEAR
                     AGDPLQFERHGYFVADRDGFNRTVTLRDSWAKA"
     gene            5423..6439
                     /locus_tag="AC16O9_03750"
     CDS             5423..6439
                     /locus_tag="AC16O9_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6515763.1"
                     /translation="MKAVGHYKYLPIDDPESLLDVTLDAPEPKGRDLLVEVKAVSVNP
                     VDTKRRAAGNRPDTELKILGWDAAGIVQATGPEAELFKPGDKVYYAGSNQRPGANCQL
                     HLVDERIVGPKPTNLSFAEAAAMPLTTITAWEALFDRMGISKSGAHASRRLLILGGAG
                     GVGSIAIQLAKKLARLTVIASASRPDSIAWVRGLGADAVVDHTQALAPQLAALGAPEA
                     DYVLCCNNTDAIFPELARLIAPQGRICSIVRTLRPVDLAPLQEKSVSFAWEGMFTRST
                     FRTPDMQAQHNLLEDAAGLLEAGTLVTTFRANLGRICAENLRQAHQRLEHGHTVGKLV
                     LEGF"
     gene            complement(6436..8478)
                     /locus_tag="AC16O9_03755"
     CDS             complement(6436..8478)
                     /locus_tag="AC16O9_03755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6515764.1"
                     /translation="MPGSAVSGRLFGLIWPLLAAMVLLLLVAVLGMQVLSVIRAYIAG
                     ESHWSKAQKDAVSQLVHYAQTGDAMAYRMYQSSIAVNLGVRRARLAMEGPEPDPETAR
                     LGLLEGRNHADDVGAMVALYPYFRHEPDVANAIDAWVRADALLVRLHGLAQALRVEVE
                     VRGAGTPRTHDLANEAIALGKLITPLAQEYADAMSAAARRTSQLIALAGALLTLLLAA
                     GGILISRRSLARLQRAEAQQRSAEQRLAQLAQFDALTGLANRTLFQDRLAQAIARAHR
                     SERRVALLFLDLDRFKEINDSLGHEAGDCVLQEAGVRLRRQLREGDSVARLGGDEFTV
                     ILEDVESAEQVQEVAKKLLRAFAEPMSFAGRDLFVTLSIGIALYPRDGDDADSLLKHA
                     DTAMYQAKHEGRDNLQFYAAAMSAAANERLSLEGSLRQALERSEFVLHYQPVVRLASG
                     EISSVEALLRWRHPQDGLVPPGRFIGAAEQNGLIVPIGAWVLQEACAQAARWQAAGLR
                     PVRVAVNLSARQFRRPGLVDTVRAALRAAALHPQWLLLEITETLLMDNPTAGSAVLGA
                     LRDLGAHMALDDFGTGYSSLAYLKHFPLDFIKIDHSFVRDIPGDADDASIVKATIGLA
                     ASLGMLTTAEGVETQAQLAFLKEHGCRYGQGFLFSPAVEADAFAAMLREERILGGA"
     gene            8593..9096
                     /locus_tag="AC16O9_03760"
     CDS             8593..9096
                     /locus_tag="AC16O9_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR6515765.1"
                     /translation="MNRPPIRTPAELYAHAIAIEREAAERYAELAQFMADLGNDEVAA
                     LFRTLAVYEAEHLDTLEARTAGVEVPKFAPAEYAWLDASAPETVARELVFRLLTPRHA
                     LAIALDAERRAHQFFAEVHASAADPALRALAQEMALEEEGHIAMVEQAIARTPDVRVD
                     WTTVFGD"
     gene            9144..11186
                     /locus_tag="AC16O9_03765"
     CDS             9144..11186
                     /locus_tag="AC16O9_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein"
                     /protein_id="HIR6515766.1"
                     /translation="MARLFSNRDRPFDLGPLPVELLPRDAGAPALQARPPADASAAAP
                     DSIAAALPEYRELFAKFLDGEVARSRAPVPEDLEQRAQNLKASAYFLDVTLAGGCRLE
                     AGDWLTQERPAHSHAFVFLVEFGRETQPGEAGDAWIRGTNSAKTDLRCAEVAVVMSGY
                     VRALGWHARGHFAEHTQVSIERLAQRAGLVRAAGGVLAAPFLKRGFRLGVVTTDYEFA
                     ADTPIASLDWPSLDAYMGKPGTRPGWTEAEQDRRPLHLGRYEMERIPRVDEPTTRVLR
                     EEIKRVPKRADLFSRALAGDLGERPAHERRRFAVKHPLAFAMTPLIRNMVPLQGGREP
                     LAGSSLKDARRNAEAVKALGYYLGADLVGICRAEPWMYYSHGDEAGEPIDAYHPYAVV
                     MLIDQGFETMEGSSGDDWVSGAQSMRAYMRGAHLAGVMAAHLRRLGHSSRVHSNAHSE
                     LLHLPAMLMAGLGEMSRIGELVLNPFIGPRSKSVVFTTDLPLEVDRPVDFGLQAVCRM
                     CQKCARECPCNAIPFGAKVMFNGYEIWKPDVEKCGRYRLTNARGSACGRCMKTCPYNR
                     EDLVESEKLLWLSIDVPAARRALIDYDDATGGGARNPLKRWWFDLEIVDGVALKPASA
                     NERDLDLGRGEKLAKAQKLAFFPPELQPKGGTTVRDVVPIDRQAGLAAYAAAKRMP"
     gene            11186..11542
                     /locus_tag="AC16O9_03770"
     CDS             11186..11542
                     /locus_tag="AC16O9_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515767.1"
                     /translation="MRAVLIAVLLLAQGAFAGEADVVDVKVRRNSAGTYDFDVTVKSV
                     DKGWHHYADAFEVIGPDGAVLGRRVLYHPHETEQPFTRDLYGVRIPAGVTHVTVRARH
                     KPKGYDGKTMRATVPR"
     gene            11681..12496
                     /locus_tag="AC16O9_03775"
     CDS             11681..12496
                     /locus_tag="AC16O9_03775"
                     /inference="COORDINATES: protein motif:HMM:NF017373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6515768.1"
                     /translation="MNNFGDSFAGAAGALLERLAAHLPSLIGALLLLAAGWLLARLLR
                     VLTGRGMQLAESLIERLAGPSRLRVGRSANVLGTVVYWVVLLFFVTAATHVLGLQTFT
                     DWLARLLDYLPTLAAGVLIVVAGYVLASFVADLVHATAVRLQPAQRTALARLAQGATL
                     VAAILVGADQIGIRVTWLAILAAVVIASVLGGVTIAVSLGARGYVANLIGAHYLRQAF
                     QIGQRVRVAGFEGRIVEVSATSLVLEAKEGRVALPGRVYHDEPIVLLTGDGNG"
     gene            12489..13340
                     /locus_tag="AC16O9_03780"
     CDS             12489..13340
                     /locus_tag="AC16O9_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR6515769.1"
                     /translation="MADAETLSFAFMRAHPAEAARVLESLAPAAAAGLLDRVPARLAA
                     PVLSAMLPNAAARSLAGLDDEQVLALLGELGTQPVVAVLRHVAEPRRARLIAGLPTAA
                     ALASQLLLGYIEDTVGAWTDPDVLALPGATRAVEALDRVRHIEATVQRVFVTGAGGRL
                     EGWVPLPLLLRAPEGATLASIMQRPEAVISAQTPLAGALRHPGWERASLLPVLEAGER
                     LVGVLTHDALTRALGRTAPAPRAAPGETLAGMMALGYWDALSGGAEAMVTLLPKVETV
                     GRRVDGR"
     gene            13330..>14342
                     /locus_tag="AC16O9_03785"
     CDS             13330..>14342
                     /locus_tag="AC16O9_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6515770.1"
                     /translation="MAAEALPAVAALNQRFLLDFPGEAARELEAMPSEDAAQVLAAHA
                     PRAVVRAWEALAPDVAREVLELLPAELSRYVLAEAEPASSVAVLSQLEPEEREKLLGP
                     LDKEVARELRELVAYPQDSAGRLMDARVSPLRAGMTVGDAIERLRAIRRRGLRELYVV
                     DDDGRLAGRVEMQDLALAERARPLGDITRGVVAAVRDLDPREEVVDILQREPITVLPV
                     VNHAGRFIGVIRQAELMAAVEEETSLDIQTMVGASPDERALSRPSFAVKKRLPWLQIN
                     LLTAFLAAAVVGLFESTIAKFTALAVLLPVVAGQSGNAGAQALAVTMRGLVLREISLR
                     HWPA"
BASE COUNT         2167 a         4909 c         5175 g         2091 t
ORIGIN      
        1 tgccgcgcat tatgcccaat tgcgaccgcg ccgtcccggc gccgggaacc gccgcgcaag
       61 cgcccggtcc aagcggcatc cggaattcgc aggagacgca acatgcagac caggaagatc
      121 gtgatattgc tggcggcgct ggcgatgtcc gcaatgggcg cgatcgtggg cgcgcagtac
      181 ctcaacacag gcgtcgcgca ggcggcggcg cccgtagccg cggcgcagct gccggatttc
      241 gccggcatcg tgcagcgcta cggccctgcg gtcgtcaaca tcgccacgtc gggcacgcgc
      301 aaggcgggcg cgcagcaggc ggcgccggag ctgttccgcg ggttgccgtt ccagttcggt
      361 acgccgatgc ccgagatgcc cgttcagggc cagggctcgg gcttcatcct cagcaccgac
      421 ggcatcgtgc tcaccaatgc ccacgtcttg cagaacgcga ccgaggtcac ggtcaagctc
      481 accgaccggc gcgagttcaa ggccgaggtg ctcggcaccg acccggcgac cgacatcgcc
      541 gtgctgcgca tcgatgcgcg cgagctcccc gtggtgaggc tgggcgaccc gaagcgcgcg
      601 cgggtgggcg actgggtggt ggccataggc tcgccgttcg gcttcgagaa cagcgtcacg
      661 gcgggcatcg tgtccgccaa gggccgctcg ctgccgggcg atatctacgt gccgttcatc
      721 cagaccgacg tggctgtgaa tccgggcaat tccggcgggc cgctgttcaa cctcgacggc
      781 gaagtgatcg gcatcaactc gcagatctac agccgctcgg gcggctacca gggcctgtcg
      841 ttcgcgattc ccatcgacat ggcgctgcgc gtcaaggacc agatcgtggc caccgggcat
      901 gccacgcatg cgcgcctcgg cgtcaacgtg cagaccatga accaggcgct cgccgagtcg
      961 ttcggcctcg agcgtccgca gggcgcgctg gtcgccgccg tggcgccgga cagccccgcg
     1021 cagcacgcgg gattgcagcc gggcgatgtg gtggtgaagt tcaatggccg gccggtcggc
     1081 gattccggcg agctggccgc gatggtgggc gagtcgacgc cgggcgcgaa ggtgcagttc
     1141 gaagtgctgc gcaaggggga gcggcgcacg ctcgctgcca cgctcggcac cgccgaggac
     1201 aagtcgaagc agcaggccgc tgacgacgag gcggcgggca agggccggct cggccttgcg
     1261 gtgcgcccgc tcacgccgca ggaactcgag gcagctcacg tgccgggcgg gctgctggtg
     1321 cagggcgtga acggcgccgc ggcgcgcgcc ggcattgctc cgggcgacat cgtgctgcag
     1381 gcggacggca aaccggtggc gagcgtcgag gacctcagaa gcgcgacgca atcgggcaag
     1441 accattgcgc tgctggtgca gcggggcgag cggcggctgt tcgtgccgct gcaggcgggc
     1501 tgatcccggc tagcggcggc gcttctcgta ccagcgcgac atcgcctcca tcgccagcca
     1561 cgaggccgcc gccgcggcga gcagcacgac ctcggcggcg ttccagcccc agccatcgac
     1621 gatcagcgcg aagatcgtcg gcgacgcggc gttgacgatg aggatgggcg tggcgagcac
     1681 accgagcact gcgccgtagc ctgtggcgcc gaagagcgcc agcggcaccg cgccgcgcac
     1741 gatggtgatc acaccctgcg aggcgcccat cagcagcgta aaggcaatca ccgcctgcag
     1801 gttgccgccg gagaacaaga gcacggcaaa ggacgccggc agcacgccga tggcgatgcg
     1861 cgcgaccgtg atggcgcgaa gattgcgccc gaagacgatc tccacgaggc ggccgccgaa
     1921 ctgggcgaag cctttcatcg aggcgaccca cacggcggcc gcggtagcga gcccggcggc
     1981 ttccagcagc ggcaccagct gcacggtgac cacgccgaag acgaagccgt tcagcgacat
     2041 cacgagcgca aacagcgcga tggcgatggt gcgcaccttg ccctgcagcg gcgcgccgtc
     2101 caccgagggc ttcgccgccg cggcctcgtg tccgccggcc ggctcgcggc gcgccagccc
     2161 gaaccagttg agcggcaggc agaccgagag gttgatgagc gcgaaccagc cgagcgtctc
     2221 gcgccagccg agcgcctcgt tgaggtagtg gccgacgacc cagaacaccg tggaggcgaa
     2281 cgcgccgaag agcgtcaggt aggagatggc gcgccggccg cgggagggca cgacctgcac
     2341 cagcgcggcg aaggcggcgt cgtacaggca caggcgcatg ccgacgccga gaaacgccca
     2401 cacgccgagg taggcggctt cgctcgtcac aagcgacagc agcagcaggc ccgcagagac
     2461 cagcacggta ccggtgctca tcaccgcctg cgcgccgaag cggtcaatgg cgcgtcccgc
     2521 ccaggtcgac accgccccca tcacgagcag cgacacggtg aagccgaaat agatcaggct
     2581 caggctccag ccggtatccc gggcgatcgg gttggcgagc acgcccaggc agtagtagct
     2641 cgtgccccag gcggtgatct gcgtgatgcc cagcgcgcag acggcgaaga ggaggggatc
     2701 ggggcgggag cgcatggccg cgcgtatcgt aagacacggc cggcatccgg cgcgggtcgg
     2761 ttcacaccac ctgcgctaag ctagccgcat ggcaaccaga agatccggac tgaagcgggc
     2821 cccggcgaag cgccgggtga agaaggcgca ggcgctcagc cgcgggctga cgcccgctga
     2881 gtgccggctc gacatgctct cgggggaggc cgaggaggtg cgcgagcggg tgaagcaggc
     2941 gggcggcgaa gtgatcggca cctacaagga cccgctgggc gggggcgcgc tgctctcctg
     3001 catcctgccg atctcggaag tcgagccgac gccgttccag cgcgacctct cggacgcgca
     3061 ccacaagaag ctggccgacg tcatcgacag gaccggcatg tttctcgacc cgatcatctg
     3121 cgtcacggcg cccaaggacg gcttctggac gccgaacggc atgcatcggt tgatggcgct
     3181 gcggcgcctg ggagcgaaat cggtgaccgc gctggtagtg cccaagcgcg aggtggcgtg
     3241 gcagatcctg gcgctgaaca ccgagaaagc gcacaacctc aaagagaagt ccctggaggt
     3301 gatccgcatc tatcgcgggc tgatcgagga ggacgctgcg cggccggagg ccggtttttc
     3361 cttctacctg gaggaggcgt ccttcgtgac gatggggctg tgctacgaaa agaacgccaa
     3421 gttcgccggc ggcgcgtaca acccggtcgt gcggcggctg gagaagttct ccgacgagcc
     3481 cctgtcgaag gcactgaagt cacacgagaa gcacgccgcg atgctgcagg agctggacga
     3541 gaacgtggcc gaggtgatca agaagctgaa ggcgcgcggg ttccagagtc cttatctgcg
     3601 cgcgttcgtg gtcgcgaggg tgaatccgct gcgcttcatg cagggcgagc cgccggcgct
     3661 cgaggacgtg ctgaagacga tgatcgggcg ggcggcgaag ttcaatacgg aaaaggtgaa
     3721 gcaggaagac ctggcgagcg cgggtggcgc gcccgacgag gcttaggcct tcgcccagga
     3781 gtcgcgcaac gtcacggtgc ggttgaagcc gtcgcggtcg gcgacgaagt agccgtggcg
     3841 ctcgaattgc agcggatcgc cggcgcgcgc ctcctgcagg cacggctcga gcttggcccc
     3901 gatcgtcttc ttcgagccgg gattcagtcc gtcgctttgc aggtctgcga acagccggtc
     3961 gtacaggtga acgctggccg catgcgcgtg ctttgcgctc acccagtgga tgttgccttt
     4021 cactttgtac ttgtccgcgc ccggcgtgcc tgaccgggag tcctgcaggt acgtgcagcg
     4081 cacggtatcg gtcgccttgt cgtacccgat gcacttgacg acgtagccgt agcgcaggcg
     4141 cacctggttg cccggataga gacgaaagta tcccttcggc ggcgcttcct ggaaatcctc
     4201 gcgctcgatc cacagctcgc cggagaacgg caccgagcgc ttgcccatct gcggtttctg
     4261 cggatggttc ggcacctcga ccgtctcctc tatcccggcg ggatagttct cgatcaccag
     4321 cttgaccgga tccagcacgg ccatgcgccg cggcgccacc tcattcaggt gctcgcgcat
     4381 ggcgtcctcg agcacggaga aatcgatcac ctgatttgcc ttcgagatgc cgatgcgctc
     4441 ggcaaagcgc cggaacccct ccggcgtata gccgcgccgg cgcgcacccg ccagcgtcgg
     4501 caggcgcgga tcgtcccagc cgtcgacgtg gcccttgtcg acgagctcga ccagacggcg
     4561 cttcgacagg acgacatgcg tcaggttgag ccgggcgaac tcgatctgct ggggcagggg
     4621 tcgcttgagc aggccgccgt cggcaagctt gcccagcagc cagtcgtaga acggccgctg
     4681 gtcctcgaac tccagagtgc agagcgagtg cgtgatgccc tcgagcgcat cctcgatcgg
     4741 gtgcgcgtag gtgtacgttg gatagatgca ccagttgtcg ccggtgcggt ggtgcgccac
     4801 gtgcttgatc cggtacagca ccgggtcgcg caggttgatg ttgggcgaag ccatgtcgat
     4861 cttcgcgcgc agcacgtgcg tcccgtcggg gtgctttttg tcccgcatct cgcgcaggag
     4921 cgcgaggttc tcgccgggcg agcggctgcg atacgggctg tccgtgcccg gctcggtgaa
     4981 cgagccgcgc ttgtgctgca tctgctcggc gctctggctg tcgacgtagg cgaggccctg
     5041 ctgcgccaga tgctccgcgc accgatacat gaagtcgaaa tagtccgagg cgtacaacgc
     5101 cttgccgtag tcgtacccca gccagcgcac catgtcctcg atcgaatcga cgtaggcctg
     5161 ctcctccttc tccgggttgg tgtcgtcgaa gcgcaggttg cagcgcccgc cgtattcctg
     5221 cgccagaccg aagttgagca cgatcgactt ggcgtggccg aagtgcaggt agccgttcgg
     5281 ctcgggcggg aagcgggtca cgaggcgctt gtgcttgccc gaggcgaggt cggcctcgat
     5341 cacctgcttg atgaagttgg acgtggaagg cggctgcggc ttgctcacgc cggaattcta
     5401 ctagcgcggt aacatcgcca tgatgaaagc cgtcggccac tacaagtacc tgcccatcga
     5461 cgatcccgag tcgctgctcg acgtcacgct cgatgccccc gagccgaaag gccgcgacct
     5521 gctggtcgag gtgaaggcgg tgtcggtgaa cccggtggac accaagcgcc gcgccgcggg
     5581 caaccgtccc gacaccgagc tgaagattct cggctgggac gcggccggca tcgttcaggc
     5641 gacgggtccg gaagccgagc tcttcaagcc gggcgacaag gtctactacg cgggcagcaa
     5701 ccagcgcccg ggcgcgaact gccagctgca cctcgtcgac gagcgcatcg tcggcccgaa
     5761 gcccacgaat ctctccttcg ccgaggccgc ggcgatgccg ctcaccacca ttaccgcctg
     5821 ggaagcgctg ttcgatcgca tgggcatctc caagtccggc gcccacgcca gccggcgcct
     5881 gctgatcctc ggcggcgcgg gcggcgtggg ctcgatcgca atccagctgg cgaagaagct
     5941 cgcgcgcctc acggtcatcg cgagcgcttc gcggcccgac tcgatcgcct gggtcagggg
     6001 cctcggcgcc gacgccgtcg tcgaccatac gcaggcgctg gcgccgcagc tcgccgcgct
     6061 cggcgcgccg gaagccgact acgtcttgtg ctgcaacaac accgacgcga tcttccccga
     6121 gctggcgcgc ctcatcgcgc cgcagggccg catctgctcc atcgtgcgca cgctgcggcc
     6181 ggtggacctg gcgccgctgc aggaaaagag cgtgagcttc gcgtgggaag gaatgttcac
     6241 gcgctcgacg ttccggacgc cggacatgca ggcgcagcac aacctgctcg aggacgccgc
     6301 gggactgctc gaggcgggca ccctggtgac gacgttccgc gccaacctcg ggcggatctg
     6361 cgccgagaac ctgcgccagg cgcaccagcg cctcgagcac ggccacacgg tcggcaagct
     6421 ggtgctggag ggcttctagg cgccgccgag gatacgctcc tcgcgcagca tcgccgcgaa
     6481 cgcatccgcc tccaccgccg ggctgaacag gaatccctgc ccgtagcggc acccgtgctc
     6541 cttcaggaac gcgagctgcg cctgcgtttc gacgccttcc gcggtggtca gcatgccgag
     6601 gcttgcggcg aggccgatgg tggccttgac gatcgacgcg tcgtcggcat cgcccggaat
     6661 gtcgcgcacg aacgagtggt cgatcttgat gaagtcgagc gggaagtgct tcaggtaggc
     6721 gagcgaggaa tacccggtgc cgaagtcatc aagcgccatg tgcgcgccca ggtcgcgcag
     6781 cgcccccagc accgcgctgc ccgcggtcgg gttatccatc agcagggtct cggtgatctc
     6841 cagcagcagc cactgcggat gcagcgccgc ggcgcgcagc gccgcgcgca ccgtgtccac
     6901 cagaccgggg cgccggaact ggcgtgccga caggttgacc gccacgcgca ccggccgcag
     6961 gcccgccgcc tgccagcgcg cagcctgcgc gcaggcttcc tgcagcaccc aggcgccgat
     7021 cggcacgatg aggccgttct gctcggccgc gccgatgaag cgccccggcg gcaccaggcc
     7081 gtcctgcggg tggcgccagc gcagcagcgc ctcgacgctc gagatctcgc cgctcgccag
     7141 ccgcaccacc ggctggtaat gcagcacgaa ctcgctgcgc tccagcgcct gccgcagact
     7201 gccctcgagc gagaggcgct cgttcgcggc cgcgctcatg gcggccgcgt agaactgcag
     7261 gttgtcgcga ccttcgtgct tcgcctggta catcgcggtg tcggcgtgct tgagcaggct
     7321 gtcggcgtcg tcgccgtccc tggggtagag cgcgatgccg atcgagagcg tgacgaacag
     7381 gtcgcggccg gcgaaggaca tcggttcggc gaaggcgcgc agcagcttct tcgccacctc
     7441 ctgcacctgc tcggcgcttt ccacgtcttc caggatcacc gtgaactcgt cgccgcccag
     7501 gcgcgccacc gagtcgccct cgcgcagctg ccggcgcagg cgcacaccgg cctcctgcag
     7561 cacgcagtcg ccggcttcat ggccgagcga gtcgttgatc tccttgaagc ggtcgagatc
     7621 gaggaacagc agcgcgacgc gccgctcgct gcggtgcgcg cgcgcgatcg cctgcgccag
     7681 gcgatcctgg aacaacgtgc ggttggcgag gccggtgagg gcgtcgaatt gcgcgagctg
     7741 ggccaggcgc tgctcggcgg agcgctgctg cgcctcggca cgctgcaggc gcgcgaggct
     7801 gcgccgggag atgaggatgc cgccggcggc aagcagcagc gtcagcagtg cgccggccag
     7861 ggcgatcagc tgcgaggtgc gccgggcggc ggccgacatc gcgtcggcat actcctgcgc
     7921 gaggggagtg atcagcttgc cgagcgcgat cgcctcgttg gcgagatcgt gcgtgcgcgg
     7981 cgttccggcg ccgcgcacct cgacctcgac gcggagcgcc tgcgccaggc catgcagccg
     8041 caccagcaac gcgtcggcgc gtacccaggc gtcgatggcg ttcgccacgt ccggctcgtg
     8101 gcggaagtat ggatacaacg ccaccatggc gccgacgtcg tcggcatggt tgcggccttc
     8161 gagcagaccg aggcgcgcgg tctcgggatc gggctccggg ccttccatgg cgagccgagc
     8221 gcggcggacg ccgaggttga ccgcgatgct cgactggtac atccggtagg ccatggcgtc
     8281 gccggtctgc gcgtagtgca ccagctggga cacggcgtcc ttctgcgcct tcgaccagtg
     8341 gctctcgccg gcgatgtagg cgcggatcac cgacagcacc tgcatgccaa gcaccgcgac
     8401 gagcagcagc aggaccatgg ccgcgagcag cggccatatg aggccgaaca gcctgccgct
     8461 gaccgcactg cccggcatcg atgcctcccc cgtatgcggg gattatacgg agcgcggggt
     8521 gtttgatctg ggtcaagccg ggcgccgcgt tgcgacgcac agtggcggca tcgggccaac
     8581 cgccgacgcg ccatgaaccg ccccccgatc cgcacgcccg ccgagctcta cgcgcacgcc
     8641 atcgccatcg agcgcgaggc cgccgagcgc tatgccgagc tggcgcagtt catggcggat
     8701 ctgggcaacg acgaggtcgc ggcgctgttc cgcacgctgg cggtgtacga ggcagagcac
     8761 ctcgacacgt tggaggcgcg taccgctggc gtcgaggtgc cgaagttcgc gcccgccgag
     8821 tacgcctggc tcgacgcgag cgcgccggag acggtggcgc gcgagctggt gttccgcctg
     8881 ctcacgccgc gccacgcgct cgccatcgcg ctcgacgccg agcgccgcgc gcaccagttc
     8941 ttcgccgagg tgcacgcgag cgccgcggat ccggcgctgc gcgcgctcgc gcaggagatg
     9001 gcgctcgagg aagaggggca catcgccatg gtcgaacagg cgatcgcgcg cacgcccgac
     9061 gtgcgcgtgg actggaccac ggtgttcggc gactagcgcc cgggcgcgcg gcgcggcccg
     9121 ggtttttgca cgagaattgg gggatggcgc gcctgttctc gaaccgcgac cgccccttcg
     9181 acctcggtcc actgcccgtt gagctgctgc cgcgcgatgc gggcgcgccg gccctgcagg
     9241 cgcgcccgcc ggcggatgcg tcggcggcgg cgcccgattc gatcgccgcg gcgctgcccg
     9301 agtaccgcga gctgttcgcg aagttcctcg acggcgaggt cgcgcgcagc cgcgcgccgg
     9361 tgcccgagga cctggagcag cgtgcgcaga acctgaaggc gtcggcctac ttccttgacg
     9421 tcacccttgc cgggggctgc cgcctcgagg cgggcgactg gctcacgcag gagcgcccgg
     9481 cgcacagcca cgcgttcgtt ttcctcgtcg aattcggccg cgaaacgcag ccgggcgagg
     9541 cgggcgacgc gtggatccgc ggcaccaaca gcgcgaagac cgacctgcgc tgcgccgagg
     9601 tcgcggtggt catgtcgggc tatgtgcggg cgctgggctg gcacgcgcgc gggcatttcg
     9661 cggaacacac ccaggtgtcg atcgagcggc tcgcgcagcg cgcggggctc gtgcgggccg
     9721 cgggcggcgt gctcgccgcg ccgttcctga agcgcggttt ccgcctgggc gtggtcacca
     9781 ccgactacga attcgcggcc gacactccga tcgcgtcgct cgactggccg agcctcgacg
     9841 cgtacatggg caagccgggg acccggccgg ggtggaccga ggcggagcaa gaccggcgcc
     9901 ccttgcatct tggccgctac gagatggagc gcatcccgcg cgtggacgag cccacgacgc
     9961 gggttctgcg cgaggagatc aagcgcgtgc caaagcgcgc cgacctgttc tcgcgcgcgc
    10021 ttgccggcga cctcggcgaa aggccggcgc acgagcgccg ccgcttcgcc gtcaagcacc
    10081 cgctcgcgtt cgcgatgacg ccgctgatcc gcaacatggt gccgctgcag ggcgggcgcg
    10141 agccgcttgc cggctcctcg ctcaaggacg caaggcgcaa cgccgaggcg gtcaaggcgc
    10201 tcggctacta cctgggcgcg gacctggtgg gcatctgccg cgccgagccc tggatgtact
    10261 actcgcacgg cgacgaggcg ggcgagccga tcgacgccta ccacccgtac gcggtggtga
    10321 tgctgatcga ccagggcttc gagaccatgg agggcagctc cggcgacgac tgggtctcgg
    10381 gcgcgcagtc gatgcgcgcg tacatgcgcg gcgcgcacct cgccggcgtg atggccgcgc
    10441 acctgcgccg tctcggccat tcgtcgcgcg tgcactcgaa cgcgcactcg gagctgctgc
    10501 acctgccggc gatgctgatg gcgggcctgg gcgagatgtc gcggatcggc gagctggtgc
    10561 tcaatccgtt catcggcccg cgctccaagt cggtcgtctt caccaccgac ctgccgctcg
    10621 aagtggaccg gccggtcgac ttcggcctgc aggcggtgtg ccgcatgtgc cagaagtgcg
    10681 cgcgcgaatg tccctgcaac gccataccct tcggcgcgaa ggtgatgttc aacggctacg
    10741 agatctggaa gcccgacgtg gagaagtgcg ggcgctaccg cctgaccaac gccaggggct
    10801 cggcctgcgg acgctgcatg aagacctgcc cctacaaccg cgaggacctg gtggagtcgg
    10861 agaagctgct ttggctgtcc atcgacgtgc cggctgcgcg ccgcgcgctc atcgactacg
    10921 acgacgcgac cggcggcggc gcgaggaacc cgttgaagcg ctggtggttc gaccttgaga
    10981 tcgtggacgg cgtggcgctg aagccggcgt cggcaaacga gcgcgacctc gacctcgggc
    11041 gcggcgagaa gctggccaag gcgcagaagc tcgccttttt cccgcccgag ctgcaaccca
    11101 aaggcggcac cacggtcaga gatgtcgtgc cgatcgatcg ccaggcgggc ctggcggcgt
    11161 acgcggctgc gaagaggatg ccctgatgag agccgttctg atcgcagtgc tgctgcttgc
    11221 ccagggcgcc ttcgccggcg aagccgacgt cgtggacgtg aaagttcggc gcaacagcgc
    11281 cggtacctac gacttcgacg tcacggtgaa gagcgtggac aagggctggc accattacgc
    11341 cgatgcgttc gaggtgatcg ggcccgacgg cgcagtgctc ggccggcgcg tcctgtatca
    11401 cccgcacgag accgagcagc cgtttacgcg ggatctctac ggcgtgcgga tcccggcagg
    11461 cgtcacgcac gtgaccgtgc gcgcgcggca caagccgaag ggttatgacg gcaagacgat
    11521 gcgggcgaca gtgccgcgct gactcacggc agcgcgcgcg cgatctcgcc gtcggggttg
    11581 aacgcgacgg gccgcggtgc cccgggcgcc ggtccatgcc ggctccgtcg gcgcaagggc
    11641 gcgatggtct actgcaagtt catcgcgata ccatacacgc atgaacaact tcggcgattc
    11701 cttcgccggc gccgccggcg cgctgctcga gcgcctcgcc gcgcacctgc cgagcctgat
    11761 cggcgcattg ctgctgctcg ccgcgggctg gctgctcgcg cgtctgctgc gcgtgctcac
    11821 ggggcgcggc atgcagctgg cggaatcgct gatcgagcgc ctggcgggac cgtcgcgcct
    11881 gcgcgtgggc cgctcggcga acgtgctcgg caccgtcgtc tactgggtgg tgctgctctt
    11941 tttcgtcacc gcggcgacgc acgtgctcgg cctgcagacc ttcaccgact ggctggcgcg
    12001 gctgctcgat tacctgccga cgctcgccgc gggcgtgctg atcgtcgtcg ccggctacgt
    12061 cctggcgagc ttcgtcgccg acctggtgca cgccaccgcg gtgcggctgc agcccgcgca
    12121 gcgcacggcg cttgcgcgcc tggcgcaggg cgcgacgctg gtcgccgcga tcctggtggg
    12181 cgccgaccag atcggcatcc gggttacctg gctcgcgatc ctcgccgcgg tggtcatagc
    12241 ctcggtgctc ggcggcgtca ccatcgcggt gagcctgggc gcgcgcggct acgtcgcgaa
    12301 tttgatcggc gcgcattacc tgcgccaggc gttccagatc gggcagcgcg tgcgcgtcgc
    12361 cggattcgag ggccgtattg tcgaggtgag cgccacgtcg ctggtgctgg aagccaaaga
    12421 aggccgggtc gcgctgcccg ggcgcgtcta tcacgacgag cccatcgtgc tgctcacggg
    12481 cgacggcaat ggctgatgcc gaaaccctca gctttgcctt catgcgcgcg cacccggcgg
    12541 aagccgcgcg cgtgctggaa tcgctggccc cggcggccgc cgccgggctg ctcgaccgcg
    12601 tgccggcgcg gctggctgcg ccggtgctct cggcgatgct gccgaacgcg gcggcgcgca
    12661 gcctcgccgg gctcgacgac gagcaggtgc ttgcgctcct cggcgagctc ggcacgcaac
    12721 ccgtggtggc ggtgctgcgc catgtcgccg agccgcgccg cgcgcggctc atcgccgggc
    12781 tgcccaccgc ggcggcactc gcctcgcagc tgctgctcgg ctacatcgag gataccgtcg
    12841 gcgcctggac cgatcccgac gtgctggcgc tgccgggggc gacacgcgcc gtcgaggcgc
    12901 tcgatcgcgt gcgccacatt gaagccaccg tgcagcgcgt gttcgtgacg ggcgcgggcg
    12961 gccggctgga aggctgggtg ccgctgccgc tgctgctgcg cgcgcccgag ggcgcgacgc
    13021 tcgcgtcgat catgcagcgg cccgaggccg tgatctcggc gcagacgccg cttgccgggg
    13081 cgctgcgcca tccgggctgg gagcgcgcgt cgctgctgcc ggtgctcgaa gccggcgagc
    13141 gtctggtggg cgtgctgacg cacgacgcgc tgacgcgcgc gctggggcgc acggcgcccg
    13201 cgccacgcgc cgcgccgggc gaaacgctcg ccggcatgat ggcgctcggg tactgggatg
    13261 cgctgtccgg tggcgccgaa gccatggtga cgctgctgcc gaaggtcgag acggtgggcc
    13321 ggagggtcga tggccgctga agcgctgccc gcggtcgccg cgctcaatca gcgtttcctg
    13381 ctcgattttc cgggcgaggc ggcgcgcgag ctggaggcga tgccgtccga ggacgcggcg
    13441 caggttctcg cggcgcatgc gccgcgcgcg gtcgtgcgcg cctgggaagc gctcgcgccc
    13501 gacgtcgcgc gcgaggtgct cgagctgctg cccgcggagc tgtcgcggta cgtcctcgcg
    13561 gaagccgagc ccgcctcgag cgtcgccgtg ctgtcgcagc tcgagcccga ggagcgcgag
    13621 aagctgctcg ggccgctcga caaggaagtg gcgcgcgagc tgcgcgagct tgtggcctat
    13681 ccccaggact ccgcggggcg gctgatggac gcacgcgtca gccccttgcg tgccggcatg
    13741 acggtgggcg acgccatcga gcggctgcgc gccatccgcc ggcgcgggct gcgcgagctg
    13801 tacgtggtgg acgacgatgg gcggcttgcc gggcgcgtgg agatgcagga cctggcgctt
    13861 gccgagcgcg cgcggccgct cggcgacatc acgcgcggcg tcgtggctgc ggtgcgcgat
    13921 ctcgatccgc gcgaagaggt cgtggacatc ctgcagcgcg agccgatcac cgtgctgccg
    13981 gtggtgaacc acgccggacg cttcatcggc gtcatccgcc aggccgagct gatggcggcg
    14041 gtggaggagg agaccagcct cgacatccag accatggtcg gcgcgagccc cgacgagcgc
    14101 gcgctgtcgc gcccgtcgtt cgccgtgaag aaacgcctgc cgtggctgca gatcaacctg
    14161 ctcaccgcgt tcctcgccgc ggcggtggtg ggcctgttcg agagcaccat cgccaagttc
    14221 accgcgcttg cggtgctcct gccggtggtg gcggggcagt cgggcaacgc cggcgcgcag
    14281 gcgctggcgg tgaccatgcg cgggctggtg ctgcgcgaga tcagcctgcg gcactggccg
    14341 gc
//
LOCUS       DBNHKE010000060        14718 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1014986, whole genome shotgun sequence.
ACCESSION   DBNHKE010000060 DBNHKE010000000
VERSION     DBNHKE010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1014986"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="AC16O9_03790"
     CDS             complement(<1..918)
                     /locus_tag="AC16O9_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515771.1"
                     /translation="MNSTRRNVGLLAVCQALLMTNNATLIAINGLAGLALAPRAALAT
                     LPVTCWVIGGALATMLASLHMKRVGRRRGLTQGTLWGVVGALICAGAVWVQSFWLLCF
                     GALVFGVYNAYGQYYRFAAADVASADFKSTAISLVLAGGLVGGILGPTASRFTVDLLE
                     PRFTGAYLALIGFAFATMLVLRFIDIPPLSAKAQAAVGRPLREIAAQPKFIVAVMSGA
                     IGYGVMNFLMTSTPIAMGVCGHPYGDAAFVISSHVIAMFAPSFVTGRLIRRFGVLQVM
                     FCGALLNFATIGIALSGIQVAHFWWSLVLL"
     gene            956..2023
                     /locus_tag="AC16O9_03795"
     CDS             956..2023
                     /locus_tag="AC16O9_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR6515772.1"
                     /translation="MQRQRGALSQIQGRFAPEARAPFDDGWTREDQAPAPLQTTITIE
                     RARTIIARNDSPDIPFDQSINPYRGCEHGCVYCYARPSHAYLELSPGLDFETKLFAKT
                     NAAERLRAELAKPGYRVSPISLGANTDCYQPAERKHRITRQILEVLAECEHPLTIVTK
                     SALVERDLDLLAPMARKNLVKVFVSIGTLDRVLARKLEPRAASPQRRLDTLRALSQAG
                     VPCGVMVAALIPALNDKTLEHVLAEAAKAGALDAAYVILRLPNELKDLFKEWLGAHYP
                     ERAEHVMSIVRQMRGGRENDPRFHERMRGTGNHAELIDRRFDIACRRHGLNGHGGGRR
                     TPDLDCSRFVPPSPAGQLKLF"
     gene            2023..2382
                     /locus_tag="AC16O9_03800"
     CDS             2023..2382
                     /locus_tag="AC16O9_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6515773.1"
                     /translation="MRALAVTGGWLLAAAIVWLSLTPSPPRLDLESGDKLGHFLGYAG
                     LMFWFALLYRAARARIGYALLWIAMGVALEFAQQAGGYRSFELADMAANTLGVLAGAL
                     AAFSLPRAARARERETP"
     gene            complement(2336..3058)
                     /locus_tag="AC16O9_03805"
     CDS             complement(2336..3058)
                     /locus_tag="AC16O9_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR6515774.1"
                     /translation="MSGRFSDIWVYLSASPLLWLTATLLAYQGAFWIYRKANMNPLAN
                     PVAIAVAALVALLWFTGTPYRVYFEGAQFVHFLLGTATVALAVPLYANFETLKKNLVP
                     LGGALLAGSVVAAVSAVGIGWLLGASRETLLSLAPKSATAPIAMGVAEKLGGLPSLTA
                     VLVVTTGILGAVLARGTLNALRIEDHAVRGFAVGVAAHGIGTARAFQVSEKMGAFAGL
                     AMGLNGMLTAFLFPVLARLWGG"
     gene            complement(3055..3387)
                     /locus_tag="AC16O9_03810"
     CDS             complement(3055..3387)
                     /locus_tag="AC16O9_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR6515775.1"
                     /translation="MLGAFTLLLVYQLVGEVLVLVTGLPVPGPVVGMLLLFLSLAARG
                     SAPQWLRDTCQGMLAHLSLLFVPAGVGVLLHFQRLGAEWLPITVALVASTVITIGVTA
                     LVMRALQK"
     gene            3418..4755
                     /locus_tag="AC16O9_03815"
     CDS             3418..4755
                     /locus_tag="AC16O9_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6515776.1"
                     /translation="MSTARAQRLLEGPIIPTLARLAAPGAALALFQTAVSIADTYFVG
                     RLGTAALAGLALVFPLVMLMQMLSAGAMGGGVSSALARALGAGREDTAKRLIAHALVI
                     AVGGGLAFTSLLLGLGDALYGLLGGEGAALAQALAYSHVLFAGAIAVWLANTLASVLR
                     GSGNTLAPAAALAAAALVQIPLSGALTLGWGPFPRLGIAGAALAYIAAFLVASLIMGA
                     MVWREGLRPAREHWRLEWRVFREILRVGAVSSVGALQTVLTAVVLTGFVGSFGTAALA
                     GYGVGVRLELLQIPLVFAIGQALVVMVGTNVGAGRGAHAKTIAWTGTAIAAGISLAIG
                     ATVALIPGVWIRIFSDDPAVLASGSLYLRIVAPFYVLFGAGMALYFASQGAGRMVLPV
                     LAGTVRLAFVLAAGALVVALDGPLAAVFAVIAGGIALFGGLIIAAVRAARWED"
     gene            complement(4752..5543)
                     /locus_tag="AC16O9_03820"
     CDS             complement(4752..5543)
                     /locus_tag="AC16O9_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6515777.1"
                     /translation="MNFAVDGRQAFAYTAAHAPDPARPTLIFAHGAGLDHSWFGLQSR
                     YFGYHGRNALALDFPGHGRSAGPPLGTIAEMADWVLRLLDAAKLDKASVVGHSMGALV
                     ALECAARHPGRIEKIALLGIAYPMKVSDAFLGAAKENRQDAFDMDTIWGHAPQVPLGG
                     NPNPGMWMYGDTLARLARLAPGVLYSDLKACHEYAGGEAAAARVKCPVLFILGRRDQM
                     TAAKAGLAFAQKVPGAQVVQIAPSGHSLMAESPDAVLDALAAFLA"
     gene            complement(5540..6835)
                     /locus_tag="AC16O9_03825"
     CDS             complement(5540..6835)
                     /locus_tag="AC16O9_03825"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HIR6515778.1"
                     /translation="MAAGPKYPGFDTLALHAGQSPDATTGARAVPIYATTSYVFRDSE
                     HAASLFNMERAGHVYSRISNPTVAVLEERMAALEGGVGAIATASGQAALHLAVATLMD
                     AGSHIVASNSLYGGSHNLLGYTMRRFGIETTFVAPGTPENFRKAIRPNTRLLFGETLG
                     NPGLEVLDIPAVSKVAHEAGLPLLVDATFTTPYLMQPFALGADLLYHSATKFLGGHGV
                     AIGGVLVDSGRFDWENSGKFPQLTAPYAGFHNMDFEEESGTRAFLLRARREGLRDFGA
                     CMAPMTAFHILQGIETLHLRMPRHVESARKIVDFLASNVAVDKVLYPELPGHPDHALA
                     KKLLPKGCGAVFSFDLKSGREGGKRFIESLRVFSHLANVGDAKSLVIHPASTTHYRMT
                     DEDLVKAGIGPGTVRLSIGLEDAEDLVEDLARGLRAAQK"
     gene            complement(6844..7401)
                     /locus_tag="AC16O9_03830"
     CDS             complement(6844..7401)
                     /locus_tag="AC16O9_03830"
                     /inference="COORDINATES: protein motif:HMM:NF042855.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HIR6515779.1"
                     /translation="MRLEVHVHADIPILEGVSRKQIEQALAPWLEYLDAEALADVKSL
                     EAEEPGLRFDEKELMLLVCWTGEIGRSFYAALDASLENLGPYCYEAVEVDLTLYQENG
                     DQEAKLLFVGPTAQSIHEAQRRCMIEDLTAILERQFDKERIAEVTTLVNQLFDRDWKE
                     KSSGAKPQQERAFEYVARPSRKNLH"
     gene            7484..7726
                     /locus_tag="AC16O9_03835"
     CDS             7484..7726
                     /locus_tag="AC16O9_03835"
                     /inference="COORDINATES: protein motif:HMM:NF023513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 family protein"
                     /protein_id="HIR6515780.1"
                     /translation="MSALSILYDSEHYCVAEFGAGMGIELVDKQARRSGFLEGPVAAR
                     LRSSMAQLSAADSADDEVDEFLCTYEALLTHHLHLH"
     gene            7746..8894
                     /locus_tag="AC16O9_03840"
     CDS             7746..8894
                     /locus_tag="AC16O9_03840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6515781.1"
                     /translation="MVRPDYAGGGFVNLIASIVEACGGAPRHAPLASLSARELAAARN
                     LVLLIVDGLGDNYLCREGAGGALHARRRGAITSVFPSTTASAITTSYTGWAPLEHGLT
                     GWFTFFGEAGCVGSPLPYLRRGEKAALDVAPARLYRAGSFFDGLGRRSIVVSYRPIVD
                     STYNLHHCGRAERRAYDTLEGLVEQTVAAVTSSGEPKFIYAYWPEFDALSHRHGNGSP
                     EARAHCGAVDAAFAELMTRLAGTDTIVVATADHGFIDSVGADALDFADAPGLSALLRY
                     PLCGESRAAFCHVQEGRVPEFMARARDWLGERARVCASTELLEEGWFGRGDAHPRIAE
                     RIGQVALLMHGTATVKDWTPGESRHRHVGHHGGASADEMLIPLIVGSA"
     gene            8891..9679
                     /gene="pcaD"
                     /locus_tag="AC16O9_03845"
     CDS             8891..9679
                     /gene="pcaD"
                     /locus_tag="AC16O9_03845"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HIR6515782.1"
                     /translation="MKANTNGIETSYELHGREGAPWLVLSHSLACSVRMWDPQIAALK
                     GSYRILAYDTRGHGASDAPKGPYTLELLADDLKALLDHLKVKSPHYCGLSMGGMIGQT
                     FALKYPGVFKTLMLADTTSRFPPEVGALWQERIRIAEEKGMAPLVQPTLERWFTETFR
                     KSNPDAVAGIAKLIAATPAAGYAGCCHAIPKINVTARLKEIKTPILVICGDQDPGTPP
                     AMSKEIHENAPGSKLAMIPQAAHLSNLEKPAAFTQAMQEFMAAH"
     gene            9781..10056
                     /locus_tag="AC16O9_03850"
     CDS             9781..10056
                     /locus_tag="AC16O9_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6515783.1"
                     /translation="MKATAHPQVSNERVIVTEWRFAPGAETGHHVHAHDYVVVPLTTG
                     KLRLEEPEGTREVQLTAGVSYARPKGVAHNVINANAFEFRFVEIELK"
     gene            10072..10938
                     /locus_tag="AC16O9_03855"
     CDS             10072..10938
                     /locus_tag="AC16O9_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6515784.1"
                     /translation="MAITLAVLSALCFGIALIAARIGLRTLDARSGAAISIPTATLLF
                     VLAAPFAFDARDFSLDAAIVFAVVGVFFPALVTILTFRSNELLGPTVTSAVSGTAPLF
                     ALAAASLLLGEKVPAQAAIAAAGVALGIVLLSWNPGAVHRRFAGRALLWPVAGAVVRG
                     VAQVGAKAGLLLWPNPFAACLIGYAVSSATVVTADRFRGGKRRRPTPQSVLWFGMTGA
                     LNGSAMLLMYAALSMAAVSTVAPIVAAYPLVTALASALVLRGEMLTMRIFAGAAVTVG
                     AIVYLVASTGAP"
     gene            complement(10978..12066)
                     /locus_tag="AC16O9_03860"
     CDS             complement(10978..12066)
                     /locus_tag="AC16O9_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR6515785.1"
                     /translation="MPDLSVFSSSYVEARGRFIDAARANGARVYEFRDPREKGPAGEP
                     LCLDVAVAGPENAERVLVAGSGTHGIEGYPGSAAQTAWLLAGGARRLPKKTAMVLFHA
                     HNPWGFAYKARGTEENVDLNRNFIDHGKPYPPNPGYADVHPIVTPESWDDATQAEVFR
                     QLDALRERIGEKAFSTAFNGGQFSHPDGIYFGGQREQWSNYAFREAVLKHVSHARHAA
                     IIDFHTGIGPWMEHIYLCFHAEGSPARKRARDWWGERAVNLAGVTHKALADYQGVIID
                     AFSAMLPKTEVTCIVVEFGTLERKEIQRANMAGRWLRYCGARHPALARKVHEQFCEAF
                     YPSDPKWRAAVLEQSREILERGIAGIGR"
     gene            complement(12059..12634)
                     /locus_tag="AC16O9_03865"
     CDS             complement(12059..12634)
                     /locus_tag="AC16O9_03865"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR6515786.1"
                     /translation="MAKRRLIVGITGATGTAYGLRLLEELRAARGWEAHLVLTDAGVL
                     NAHQELGLKRKDIERLAHKSYHPKDIGAAIASGSFLTEGMVIAPCSMKTLAAVAHAHA
                     DDLVSRAADVVLKERRRLVLVPREAPLNLAHLRNMVQVTEMGGIVFPPAPAFYARPKT
                     IDDLVLHTVRRVLDLFGLHSPKLARWKGIDA"
     gene            12676..13887
                     /locus_tag="AC16O9_03870"
     CDS             12676..13887
                     /locus_tag="AC16O9_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515787.1"
                     /translation="MHPRLLLVVFCPFAAGFYLSYFFRNVNAVISRDLARDFDLSSAD
                     LGFLTATYLLAFAAFQLPLGVLLDRYGPRRVLAALLCIAGTGALVFALAQDFVVLSIG
                     RALIGLGVSGCLMGAIKAFTLWFPLSRLATLNGLYLAIGGLGGLSATAPVEAFIGPFG
                     WRALFIVLAAASVSAAALIFFVVPEKPLPGHGQTLRAQIADFGRIFASVPFWRIALPL
                     VICHAVYQALQGLWLAPWLYDIAGYERPAVAAALLIAAIAYTAGSVFFGVSSDRLASA
                     GVSRMTTYKVGIATSLAMFALLAAGTTTGLAAILAVYAFTSISVSLAYALMTPLFPPA
                     MTGRVITASNVMMFGTSFLFQWGIGAMLRLFPEADGRYPAESYAVAFWSLAALQLATI
                     LWLLPMRESRV"
     gene            complement(13884..14645)
                     /locus_tag="AC16O9_03875"
     CDS             complement(13884..14645)
                     /locus_tag="AC16O9_03875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6515788.1"
                     /translation="MDKVVVVTGAARGIGLAIARRFLAGGWRAALLDIDGPTLAAAAR
                     GLGKNVLPIECDVAEPAQVRAAIDQVVARFERIDALVNNAGIAVFKPMLETTYEEWAR
                     VMAVNLTGPFLCTQACAPAMLKGGGGSVVNIGSISGVRASTLRVAYGTSKAALEHLTR
                     QQAAELGNAGIRVNGVAPGPVDTAMAKQVHTPAIRADYHDAIPLNRYGTEEEIAAAVW
                     FLCSDQASYVNGQMLAVDGGFDAAGIGLKSLRGEA"
BASE COUNT         2163 a         5210 c         5041 g         2304 t
ORIGIN      
        1 gagcagcacc agcgaccacc agaaatgcgc cacctggatc cccgagagcg cgatgccgat
       61 ggtggcgaag ttgagcagcg cgccgcagaa catcacctgc agcacgccga agcgcctgat
      121 caggcgcccg gtgacgaagg acggcgcgaa catggcgatg acgtgcgagg agatgacgaa
      181 cgccgcatcg ccgtatgggt ggccgcagac gcccatcgcg atcggcgtcg aggtcatgag
      241 gaaattcatc acgccgtagc cgatggcacc cgacatgacg gcgacgatga acttgggctg
      301 tgccgcgatc tcgcgcagcg gccgccccac cgcggcctgc gccttcgccg aaagcggcgg
      361 gatgtcgatg aagcgcagca cgagcatggt ggcgaaggcg aagccgatga gcgcgaggta
      421 cgcgccggtg aaccgcggct ccaggagatc caccgtgaaa cggctggccg tcggcccgag
      481 gatgccgccg accaggccgc cggcgagcac cagcgaaatg gcggtgctct tgaagtcggc
      541 gctcgccacg tcggcggcgg cgaagcggta gtactggccg tacgcgttgt acacgccgaa
      601 cacgagcgcg ccgaagcaca ggagccagaa gctctgcacc cataccgcgc cggcgcagat
      661 cagcgcaccc accacgcccc agagcgtgcc ctgggtcagc ccgcgccggc ggccgacgcg
      721 cttcatgtgc agcgaggcaa gcatggtggc gagcgccccg ccgatcaccc agcaggtcac
      781 gggcagcgtg gcgagcgccg cgcgcggcgc gagcgcgagg cccgccaggc cgttgatggc
      841 gatcagcgtg gcgttgttgg tcatgagcaa cgcctggcag acggccagca atcccacgtt
      901 gcggcgggta ctgttcatgt atacagtatc ggcaggaccg gccattctag aacgaatgca
      961 acgccagcgc ggcgcgcttt cgcagatcca gggacgcttc gcgcccgagg cgcgcgcgcc
     1021 cttcgacgac ggctggacgc gcgaggacca agcgcccgcg ccgctgcaga ccaccattac
     1081 gatcgagcgc gcgcgcacga tcatcgcgcg caacgactcg cccgacattc cgtttgatca
     1141 gtccatcaat ccttatcgcg gctgcgagca tgggtgcgtg tactgctacg cgcgcccgag
     1201 ccacgcctac ctcgagctct cgccggggct cgacttcgaa acgaagctct tcgccaagac
     1261 gaacgccgcg gagcggctgc gcgccgagct ggccaaaccg ggataccggg tctcccccat
     1321 ttcccttggc gccaacaccg actgctacca gccggccgag cgcaagcacc gcatcacgcg
     1381 gcagatcctg gaagtgctcg ccgaatgcga gcacccgctc accatcgtca ccaagtccgc
     1441 gctggtcgag cgcgacctcg atctcctggc gccgatggcg cgaaagaatc tggtgaaggt
     1501 gttcgtctcc atcggaacgc tcgaccgtgt gctcgcgcgc aagctcgagc cgcgcgccgc
     1561 gagcccgcaa cggcggctgg ataccctgcg cgccctgtcg caggccggcg taccctgcgg
     1621 cgtgatggtc gcggcgctga tcccggcgct gaacgacaag accctcgagc acgtgctggc
     1681 ggaggccgca aaggcgggcg cgctggacgc ggcctatgtg atcctcaggc tgccgaacga
     1741 gctgaaggac ctgttcaagg agtggctcgg cgcgcactac ccggagcgcg ccgagcacgt
     1801 gatgagcatc gtgcggcaga tgcgcggcgg gcgcgagaac gacccgcgct ttcacgagcg
     1861 catgcgcggc acgggcaacc acgcggagct tatcgacagg cgcttcgaca tcgcctgcag
     1921 gcgccacggg ctgaacggcc acggcggcgg gcggcgcacg ccggatctgg actgcagccg
     1981 ctttgtcccg ccttcgccgg ccggccagct gaagctcttc tgatgcgcgc cctcgccgtt
     2041 accggtggct ggctgctcgc cgccgcgatc gtctggctct cgctcacgcc gtcgcctccg
     2101 cgcctggacc tcgagagcgg cgacaagctt gggcatttcc tcggctacgc cggcttgatg
     2161 ttctggtttg cccttctcta ccgggccgcg cgggcgcgca tcggctacgc gctgctctgg
     2221 atcgcgatgg gcgtcgcgct cgagttcgcg caacaagccg gcggttaccg cagcttcgag
     2281 ctcgccgaca tggcggccaa cacgctcggc gtgctcgccg gggcgctcgc cgcgttcagc
     2341 ctccccagag ccgcgcgagc acgggaaaga gaaacgccgt aagcatcccg ttcagcccca
     2401 tggcgaggcc ggcaaaggcc cccatcttct ccgacacctg gaaggcgcgc gccgtgccga
     2461 tgccgtgggc cgccacgccc accgcgaagc cccgcaccgc gtggtcctcg atgcgcaggg
     2521 cgttcagcgt gccgcgcgcg agcacggcgc cgaggatgcc ggtggtgacc accagtaccg
     2581 cggtgagcga gggcagcccg ccgagctttt ccgccacgcc catggcgatc ggcgcggtcg
     2641 ccgacttcgg cgcgagcgac agcagcgtct cgcggctcgc gccgagcagc cagccgatgc
     2701 ccaccgccga cacggccgcg accaccgagc ccgcgaggag cgcgccgccg agcggcacga
     2761 ggttcttctt cagcgtctcg aagttcgcgt acagcggcac ggcgagcgcc acggtcgcgg
     2821 tgccgagcag gaagtgcacg aactgcgcgc cctcgaagta cacgcggtac ggcgtgccgg
     2881 tgaaccacag cagcgccacc agcgccgcca cggcaatcgc caccgggttg gcgagcgggt
     2941 tcatgttcgc tttgcggtag atccagaagg cgccctggta ggcgagcagc gtcgccgtga
     3001 gccacagcag cggcgaggcc gacaggtaca cccagatatc ggagaagcgc ccgctcattt
     3061 ctgcagcgcc cgcatgacga gcgcggtcac gccgatcgtg atcacggtgc tcgccaccag
     3121 cgccacggtg atgggcagcc actcggcgcc caggcgctgg aagtgcagca gcacgccgac
     3181 tccggcgggc acgaacagca gcgacaggtg cgcgagcatc ccttggcagg tatcgcgcag
     3241 ccactggggt gccgagccgc gcgcggcgag cgacaggaaa aggagcagca tcccgaccac
     3301 cggccccggc accggcaggc cggtgacgag caccagcact tcgccgacca gctggtagac
     3361 cagcagcagg gtgaacgcgc cgagcatgcg gggggaatct tacgttaggc tcgccgcgtg
     3421 agcaccgcgc gcgcgcagcg cctcctcgag gggccgatca tccccacgct cgcgcgcctc
     3481 gccgcgccgg gggcggcgct cgcgctgttc cagaccgcgg tgtcgatcgc cgacacctac
     3541 ttcgtcggcc ggctcggcac cgcggcgctc gccggcctcg cgctggtctt cccgctggtg
     3601 atgctgatgc agatgctctc ggccggcgcg atgggcggcg gcgtctcctc ggcgctcgcg
     3661 cgcgcgctcg gcgcgggccg cgaggacacc gcgaagcgcc tcatcgcgca cgcgctggtc
     3721 atcgcggtgg gcggcgggct cgcgtttacg tcgctgctcc tcggcctggg cgatgcgctc
     3781 tacggcctgc tcggcggcga aggcgccgcg ctggcgcagg cgctcgcgta ctcgcatgtg
     3841 ctgttcgcgg gcgcgatcgc cgtgtggctc gccaacaccc tggcgagcgt gctgcgcggc
     3901 agcggcaaca cgctcgcgcc tgccgctgcg cttgccgctg cggcgctggt gcagatcccg
     3961 ctctcgggcg cgctcacgct cggctggggg ccgtttccgc gcctgggcat cgccggcgcg
     4021 gcgctcgcct acatcgccgc attcctcgtc gcgagcctga tcatgggcgc gatggtgtgg
     4081 cgcgagggcc tgcgccccgc gcgcgagcac tggcgcctgg aatggcgcgt gttccgcgag
     4141 atcctgcgcg tcggcgcggt gtcttcggtc ggtgcgctgc agacggtgct caccgcggtg
     4201 gtgctgaccg gcttcgtcgg cagcttcggc accgcggcgc tcgccggcta cggcgtgggc
     4261 gtgcgcctcg aactgctgca gatcccgctg gtcttcgcca tcggccaggc gctcgtcgtc
     4321 atggtcggca ccaacgtcgg cgcggggcgc ggcgcgcacg cgaagacgat cgcctggacc
     4381 gggacggcga tcgcggccgg catctcgctt gccatcggcg cgaccgtcgc gctcattccc
     4441 ggcgtctgga tccgcatctt cagcgacgac ccggcggtgc tcgccagcgg gagcctctac
     4501 ctgcggatcg tcgcgccgtt ctacgtgctg tttggcgccg gcatggcgct gtatttcgcc
     4561 tcgcagggcg cggggcggat ggtgttgccg gtgctggccg gcacggtgcg gcttgcgttc
     4621 gtgctggcgg cgggtgcgct ggtggtggcg ctggacgggc cgctcgccgc ggtgttcgcg
     4681 gtgatcgcgg gcggcattgc gctcttcggc ggcctcatca tcgccgcggt gcgcgcggcg
     4741 cgctgggaag actaggcgag aaaggcggcg agcgcgtcga gcaccgcgtc gggcgactcg
     4801 gccatcagcg agtgcccgga gggcgcgatc tgcaccactt gcgcgccggg caccttctgc
     4861 gcgaacgcga ggcccgcctt cgccgcggtc atctggtcgc ggcgcccgag gatgaagagc
     4921 acggggcact tcactcgggc cgccgccgct tcgccgcccg cgtactcgtg gcaggccttg
     4981 agatcgctgt agagcacgcc cggtgccagg cgcgcaaggc gcgccagcgt gtcgccgtac
     5041 atccacatgc cgggattggg attgccgccg agcggcacct ggggcgcgtg gccccagatg
     5101 gtatccatgt cgaaggcgtc ctggcggttc tcctttgccg cgccgaggaa cgcatccgac
     5161 accttcatcg gataggcgat gccgaggagc gcgatcttct cgatgcgccc gggatggcgc
     5221 gccgcgcatt ccagcgcgac gagcgcgccc atcgaatgcc cgacgacgct cgctttgtcg
     5281 agcttcgccg cgtccagcag ccgcagcacc cagtcggcca tctccgcgat cgtcccgagc
     5341 ggcgggcccg cggagcgccc gtgcccggga aaatccagcg ccagggcgtt gcgcccgtgg
     5401 taaccgaagt agcggctctg caatccgaac caggaatggt ccaggcccgc gccgtgcgca
     5461 aatatcagcg tcggccgcgc cggatcgggc gcgtgcgccg ccgtgtaggc gaacgcctgc
     5521 ctgccgtcga cagcgaaatt cacttctgcg cggcgcgcag gccgcgcgcc aggtcttcga
     5581 ccaggtcctc ggcgtcttcc agcccgatcg acaggcgcac ggtgcccggg ccgatgccgg
     5641 ccttcaccag gtcttcgtcc gtcatgcggt agtgcgtggt gctcgccggg tggatgacga
     5701 gcgatttggc gtcgccgacg ttggcgaggt gcgagaagac gcgcaagctt tcgatgaagc
     5761 gcttgccgcc ttcgcgcccg ctcttcagat cgaaggagaa caccgcgccg caacccttcg
     5821 gcagcagctt cttcgccagc gcgtggtcgg gatgccccgg gagctccgga tacaggacct
     5881 tgtcgacggc gacgttcgat gcgagaaagt cgacgatctt gcgcgcgctc tccacgtggc
     5941 gcggcatcct caggtgcagc gtctcgatgc cctgcaggat gtgaaacgcc gtcatcggcg
     6001 ccatgcaggc gccgaagtcc cgcaggccct cgcgtcgcgc gcgcaggagg aaggcgcgcg
     6061 tgcccgactc ctcctcgaag tccatgttgt ggaaccctgc gtacggcgcc gtgagctgcg
     6121 ggaatttgcc gctgttttcc cagtcgaagc ggcccgagtc gaccagcacg ccgccgatcg
     6181 ccacgccgtg cccgccgagg aacttggtcg ccgagtggta cagcaggtcc gcgccgagcg
     6241 cgaacggctg catcaggtac ggcgtggtga aggtcgcatc caccagcagc ggcagccccg
     6301 cctcgtgcgc caccttggac accgccggga tgtcgagcac ttccagcccg ggattgccga
     6361 gcgtctcgcc gaacaagagc cgcgtattgg gccgtatcgc cttgcggaaa ttctcgggcg
     6421 tgcccggcgc gacgaaagtc gtctcgatgc cgaagcgccg catcgtatag ccgagcaggt
     6481 tgtgcgagcc gccgtagagc gagttcgagg cgacgatgtg gctgcccgca tccatgagcg
     6541 tcgccaccgc gaggtgcagc gccgcctggc cggaagccgt ggcgatggcg ccgacgccgc
     6601 cctcgagcgc ggccatgcgc tcctccagca ccgccaccgt ggggttggag atgcgcgagt
     6661 agacgtggcc cgcgcgctcc atgttgaaca gcgacgccgc gtgctccgag tcgcggaaca
     6721 cgtacgaggt ggtggcatag atcggcaccg cgcgggcgcc ggtggtcgcg tcgggcgact
     6781 gcccggcgtg tagcgcgagc gtgtcgaagc cgggatactt tggccctgcc gccacccctg
     6841 tcgctagtgc aggttcttgc gcgaggggcg cgccacgtac tcgaacgcgc gctcctgctg
     6901 cggcttcgca ccggaggatt tttccttcca gtcgcgatcg aacaactggt tgacgagcgt
     6961 cgtcacctcg gcgatgcgct ccttgtcgaa ctggcgctcg aggatcgcgg tgaggtcctc
     7021 gatcatgcag cggcgctggg cttcgtggat cgactgcgcg gtcggcccga cgaagagcag
     7081 cttcgcctcc tggtcgccgt tctcctggta aagggtcaga tccacctcga ccgcctcgta
     7141 gcagtagggc ccgaggttct cgagcgaagc gtcgagcgcg gcgtagaagc tgcgcccgat
     7201 ctcgccggtc cagcacacga gcagcatcag ctccttctcg tcgaagcgta gccccggctc
     7261 ctcggcctcc aggctcttca cgtcggcgag cgcttcggca tccaggtact ccagccaggg
     7321 cgcgagcgcc tgctcgatct gcttgcgcga gaccccctcg aggatgggga tgtcggcgtg
     7381 gacgtggact tcgagccgca tgggacgaaa cctcacaaaa cgggagtgca gtttatcacg
     7441 cggcgggctt aactttcggc ctgccagggc gtcaaatcgt cccgtgagcg cactcagcat
     7501 cctctacgac agcgagcact actgcgtggc cgagttcggc gccggcatgg gcatcgagct
     7561 cgtcgacaag caggcacggc gcagcggctt cctcgagggc ccggtggccg cgcgcttgcg
     7621 ctcgagcatg gcgcaattga gcgccgcgga ctccgccgac gacgaggtgg acgagttcct
     7681 gtgcacctac gaggcgctgc tcacccacca cctgcacctg cactgagccg ctagaatccg
     7741 caccgatggt gcggccggac tacgcgggcg gcggattcgt caacctcatc gcctcgatcg
     7801 tcgaggcctg cggcggcgcg ccgcgccatg cgccgctggc ttcgctgtcc gcccgcgagc
     7861 tcgcggcggc gcgcaacctc gtcctgctga tcgtcgacgg cctgggcgac aactacctct
     7921 gccgcgaagg cgcgggcggc gcgctgcacg cgcggcgcag gggcgccatc acgtcggtgt
     7981 ttccttccac cacggcgagc gccatcacca cctcctacac cggctgggcg ccgctcgagc
     8041 acggcctcac cggctggttc accttctttg gcgaagcggg ctgcgtcggc tcgccgctgc
     8101 cgtacttgcg ccgcggcgaa aaggcggcgc tcgacgttgc gcccgcgcgg ctgtaccgcg
     8161 ccgggtcctt tttcgacggg ctcgggcgcc gctcgatcgt cgtgtcctac cggccgatcg
     8221 tcgattccac ctacaacctg caccactgcg gccgcgccga gcggcgcgcc tacgacacgc
     8281 tcgagggcct ggtcgagcag accgtggcgg cagtgacgtc ctccggcgag ccgaagttca
     8341 tctatgccta ctggcccgag ttcgatgcgc tgtcgcaccg ccacggcaac ggcagccccg
     8401 aggcgcgcgc gcactgcggc gccgtcgacg ccgcattcgc cgagctgatg acgcggctgg
     8461 ccggcaccga tacgatcgtg gtggcgaccg ccgaccacgg tttcatcgac agcgtcggtg
     8521 ccgacgcgct cgatttcgca gatgcaccgg gcttgtcggc gctgctgcgc tatcctctgt
     8581 gcggcgagag ccgcgccgcc ttctgtcacg tgcaggaagg gcgcgtgccg gaattcatgg
     8641 cgcgcgcccg cgactggctc ggcgaacggg cgcgcgtctg cgccagcacc gagctgctcg
     8701 aggagggctg gttcggccgc ggcgacgcgc atccgcgcat tgccgagcgc atcggccagg
     8761 tggcgctgct gatgcacggc accgcgacgg tgaaggactg gacgcccggc gaatcgcgcc
     8821 accgccacgt cggccaccat ggcggcgcga gcgcggacga aatgttgatt ccactcatcg
     8881 tggggagcgc atgaaagcga acaccaacgg catcgaaacc agctacgagc tgcacggcag
     8941 ggaaggcgcg ccgtggctcg tcctcagcca ctcgctcgcg tgcagcgtgc gcatgtggga
     9001 cccgcagatc gcggcgctga agggaagcta ccgcatcctc gcctacgaca cgcgcgggca
     9061 cggcgcgagc gatgcgccca agggcccgta cacgctggag ctgctcgccg acgacctgaa
     9121 ggcgctgctt gatcacctca aggtgaaaag cccgcactac tgcggcctgt ccatgggcgg
     9181 catgatcggt cagaccttcg cgctcaagta tccgggcgta ttcaagacgc tgatgctcgc
     9241 cgacacgacg agccgctttc cgcccgaggt cggtgcgctc tggcaggagc gcatcaggat
     9301 cgcggaggaa aagggcatgg cgccgctggt gcagccgacg ctcgagcgct ggtttacaga
     9361 aacgttccgc aagtcgaacc ccgacgccgt ggcaggaatc gcaaagctga tcgccgcgac
     9421 gccggctgca ggctacgccg gctgctgcca cgccattccc aagatcaacg tcaccgcgcg
     9481 gctgaaggag atcaagacgc cgatcctggt catctgcggc gaccaggatc cgggtacccc
     9541 gccggcgatg tcgaaggaga tacacgagaa tgcgcccggc tcgaagctcg ccatgattcc
     9601 tcaggccgcg cacctttcca acctcgagaa gcccgcggcg ttcacccagg cgatgcagga
     9661 gttcatggcg gcgcattgaa ggcggcggca gggccttgat ccccgatcct gcccccgcag
     9721 cgcacgcacc gaatacaggt cgtacaagcg ccagaccgac gagccgaaga agcagccgcc
     9781 atgaaagcca ccgcccaccc gcaggtcagt aacgagcgcg tcatcgtcac cgaatggcgc
     9841 ttcgcgccgg gcgccgagac cggccaccac gtgcacgccc acgactacgt cgtcgtgccg
     9901 ctgacgacgg gaaagctcag gctggaagag cccgaaggca cgcgcgaggt gcagctcacc
     9961 gccggcgtgt cctacgcgcg cccgaagggg gtcgcgcaca acgtcatcaa cgccaacgcc
    10021 ttcgagttcc gcttcgtgga aatcgagctc aagtagcgac gcccggaaac cgtggcgatc
    10081 acgctggccg tgctgagtgc gctgtgcttc ggcatcgcgt tgatcgccgc acgcatcggg
    10141 ctgcgcacgc tcgatgcgcg ctcgggagcc gcgatcagca tcccgacggc gacgcttctg
    10201 ttcgtgctgg ccgcgccgtt cgcgttcgac gcgcgggatt tcagcctgga cgccgcgatc
    10261 gtgttcgccg tggtgggagt gttctttccc gcgctcgtca cgatcctcac cttccgctcg
    10321 aacgagctgc tcgggcccac ggtgacgagc gcggtatcgg ggacggcgcc gttgttcgcg
    10381 ctcgccgccg ccagcctgct gctcggtgag aaggttcccg cgcaggccgc gattgccgca
    10441 gcgggcgtcg ccctcggcat cgtcctgctc tcgtggaacc cgggcgccgt gcacaggcga
    10501 tttgccggca gggcgctgct atggcccgtc gccggcgccg tggtgcgcgg cgtggcgcag
    10561 gtcggcgcca aggccggatt gctgctctgg cccaacccgt tcgcggcctg cctgatcggc
    10621 tacgcggttt cttccgcgac ggtggttacc gccgaccggt tccgaggcgg gaaacgccgc
    10681 cggccgacgc cgcagagcgt cttgtggttc gggatgaccg gcgcactcaa cggcagcgcc
    10741 atgctcctca tgtacgccgc gctcagcatg gccgcggtgt cgaccgtcgc cccgatcgtc
    10801 gccgcgtatc cgctggtcac ggcgctcgcc agcgcgctcg tgctgcgcgg cgagatgctc
    10861 accatgcgaa tcttcgcggg cgcggcggtc acggtcggcg cgatcgtcta cctcgtggcg
    10921 tcgacgggcg cgccatgacg gacatggcca ggccgacggc gccgtgctga atccggccta
    10981 gcgcccgatc ccggcgatgc cgcgctcgag aatttcgcgc gactgctcca gcaccgccgc
    11041 gcgccacttc gggtccgagg ggtagaacgc ctcgcagaac tgctcatgca ccttgcgtgc
    11101 gagcgccggg tgccgcgccc cgcagtagcg cagccagcgc cccgccatgt tggcacgctg
    11161 gatttccttc cgctcgagcg tgccgaactc caccacgatg caagtcacct ccgtcttcgg
    11221 cagcatggcc gagaacgcat cgatgatcac gccctggtag tcggcgagcg ccttgtgcgt
    11281 gacgccggcg aggttcaccg cgcgctcgcc ccaccagtcg cgcgcacgct ttcgggccgg
    11341 cgagccctcg gcatggaagc acaggtagat gtgctccatc cacgggccga tgccggtatg
    11401 gaaatcgatg atcgcggcgt gcctggcgtg cgacacatgc ttcagcaccg cctcgcgaaa
    11461 cgcgtagttc gaccactgct cgcgctgccc gccgaaatag atgccgtcgg gatgcgagaa
    11521 ctgcccgccg ttgaatgccg tggagaacgc cttctcgccg atgcgttcgc gcaatgcatc
    11581 gagctggcgg aacacctcgg cctgggtggc gtcgtcccaa gactccggcg taacgatggg
    11641 gtgcacgtcg gcgtagccgg gattcggggg atacggcttg ccatggtcga tgaagttgcg
    11701 gttgaggtcg acgttctcct cggtgccgcg cgccttgtag gcgaagcccc aggggttgtg
    11761 cgcgtggaac aacaccattg ccgtcttctt cggcaggcgc cgcgcgccgc cggcgagcag
    11821 ccacgcggtc tgcgccgccg agcccggata gccctcgatg ccgtgcgtgc cgctgccggc
    11881 gaccagcacg cgctcggcat tctcgggccc ggcgaccgcg acgtcgaggc acagcggctc
    11941 gcccgccggt cctttttccc tcgggtcgcg gaactcgtag acgcgcgcac cattggcgcg
    12001 cgcggcatcg atgaagcgcc cgcgcgcctc gacgtagctc gaggagaaca ccgaaagatc
    12061 aggcatcgat gcctttccag cgcgcgagct tcggcgagtg caggccgaac agatccagca
    12121 cgcggcgcac cgtgtgcagg acgaggtcgt cgatcgtctt cggccgggcg tagaacgcgg
    12181 gtgccggcgg gaacacgatg ccgcccatct cggtcacctg caccatgttc ctcaggtgcg
    12241 cgaggttgag cggcgcctcg cgcggcacga gcaccaggcg ccggcgctcc ttcagcacca
    12301 cgtcggcggc gcgcgacacg aggtcgtcgg cgtgcgcgtg cgccaccgcc gccagggtct
    12361 tcatcgagca cggcgcgatc accatgcctt cggtgaggaa ggagccgctg gcgatcgccg
    12421 cgccgatgtc cttggggtgg taggacttgt gcgcgaggcg ctcgatgtcc tttctcttca
    12481 gcccgagctc ctggtgcgcg ttcagcacac ccgcgtcggt gagcacgagg tgcgcctccc
    12541 agccgcgcgc cgcgcgcagt tcctcgagca gccgcagccc gtaggccgtg ccggtggccc
    12601 cggtgatgcc gacgatgagg cgccgttttg ccatgcgcgc aagcttagcc gatttggacc
    12661 cttagaatgc cgcgggtgca tccgcggctg ctcctcgtcg tgttctgccc ctttgctgcg
    12721 ggcttctacc tctcgtactt cttccgcaac gtcaacgcgg tcatttcgcg cgacctggcg
    12781 cgcgatttcg atctctccag cgccgacctc ggctttctca ccgccaccta cctccttgcc
    12841 ttcgccgcgt tccagctgcc gctcggcgtg ctgctcgacc gctacgggcc gcggcgcgtg
    12901 ctggcggcgc tgctgtgcat cgcgggcacg ggcgcgctcg tcttcgcgct ggcgcaggac
    12961 ttcgtcgtgc tctcgatcgg gcgcgcgctt atcggcctcg gcgtctcggg ctgcctgatg
    13021 ggcgcgatca aggcgttcac gctctggttt ccgctgtcgc gcctcgcgac gctgaacggc
    13081 ctgtacctcg cgatcggcgg cctgggcggg ctctccgcca ccgcgccggt cgaggcgttc
    13141 atcgggccgt tcggctggcg cgcgctgttc atcgtgcttg ccgccgcctc ggtgagcgcc
    13201 gcggcgctga tcttcttcgt ggtgccggaa aagcccttgc ccggccacgg ccagacgctg
    13261 cgcgcgcaga tcgcggattt cggccgcatc ttcgcctcgg tgcccttctg gcgcattgcg
    13321 ctgccgctcg tcatctgcca cgcggtctac caggcgctgc aaggcctgtg gctcgcaccc
    13381 tggctctacg acatcgccgg ctatgagcgc ccggccgtgg ccgcggcgct gctcatcgcg
    13441 gcaatcgcct ataccgccgg ctcggtgttc tttggcgtct cgagcgaccg gctcgcgagc
    13501 gccggcgtgt cgcgcatgac cacctacaag gtcggcatcg ccacctcgct cgccatgttc
    13561 gcgctgctcg ccgcggggac caccacgggc ctggccgcaa tcctcgcggt gtacgccttc
    13621 acctcgatct cggtctcgct cgcctacgcg ctgatgacgc cgttgttccc gccggcgatg
    13681 accgggcgcg tgatcaccgc ctcgaacgtc atgatgttcg gcacgtcgtt tctgttccag
    13741 tggggcatcg gcgccatgct gcggttgttt ccagaagccg acggccgcta tccggcggaa
    13801 agctacgccg tggcgttctg gtcgctcgcc gcgctgcagt tggcgacgat cctgtggctg
    13861 ctgccgatgc gggaatcgcg cgtctaggct tcgccgcgca gcgacttcag gccgatgccg
    13921 gcggcatcga accccccgtc caccgcgagc atctggccgt tgacatagga cgcctggtcg
    13981 ctgcacagga accagaccgc ggcggcgatc tcttcttccg tgccgtagcg gttgagcggg
    14041 atcgcgtcgt ggtagtcggc gcggatggcc ggggtgtgca cctgctttgc catcgccgtg
    14101 tccaccgggc ccggcgcgac gccgttcacg cgaatgccgg cgttgccgag ctccgcggcc
    14161 tgctgccggg tcaggtgctc gagcgccgcc ttgctcgtgc cgtaggccac gcgcagcgtg
    14221 ctcgcgcgca ccccggagat cgagccgatg ttgacgacgc tgccgccgcc gcctttcagc
    14281 atcgccgggg cgcaggcctg cgtgcatagg aacggaccgg tcaggttgac ggccatcacc
    14341 cgcgcccact cttcataagt ggtctcgagc atcggcttga acacggcgat gccggcgttg
    14401 ttcaccagtg cgtcgatgcg ctcgaagcgc gcgacgacct ggtcgatcgc cgcgcgcacc
    14461 tgcgccggct cggcgacgtc gcactcgatc ggcagcacgt tcttgcccag cccgcgtgcc
    14521 gccgccgcga gcgtcggccc gtcgatgtcg agcagggcgg cgcgccagcc gccggcgagg
    14581 aaccgccgcg cgatggcgag cccgatgccg cgcgcggcgc cggtgacgac gacgactttg
    14641 tccatggcgg agcgcagtat aagcttgcat aaatccaaag cggagcaccc catgttcgag
    14701 accaccatcg ccggcagc
//
LOCUS       DBNHKE010000061        10986 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_168642, whole genome shotgun sequence.
ACCESSION   DBNHKE010000061 DBNHKE010000000
VERSION     DBNHKE010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_168642"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="AC16O9_03880"
     CDS             <1..436
                     /locus_tag="AC16O9_03880"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052908 - 16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase A"
                     /protein_id="HIR6515789.1"
                     /translation="LLFHLARFADRLRDLHFMLQREVVERMVAPPSTAAYGRLSVMLQ
                     ARFTMQKLFRVAAGAFRPPPQVESAVVRLVPLAEPLDRDVEGFAELVRRAFSARRKTL
                     RNALGLPAEALEALGIDARLRPENLSPADYVRIARRTISTLC"
     gene            495..1013
                     /locus_tag="AC16O9_03885"
     CDS             495..1013
                     /locus_tag="AC16O9_03885"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR6515790.1"
                     /translation="MKAGHVLALLTTAALAGCQTMSDEPLRASAGLEPTKGNKVVGEV
                     NFEQAGGKVRVTAQLIRLAPNQEFGFHIHEVGDCSSGDGLSAKGHFNPYGKPHGPQGS
                     EHHAGDMPNLKADAKGKAKLSADLDIVTLTAGPANIVGRAVIVHADPDDYKTQPTGNA
                     GARLACGVIKAN"
     gene            complement(1060..1446)
                     /gene="gloA"
                     /locus_tag="AC16O9_03890"
     CDS             complement(1060..1446)
                     /gene="gloA"
                     /locus_tag="AC16O9_03890"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIR6515791.1"
                     /translation="MRILHTMLRVGNMERSVKFYTEVLGMKVLRTTDRPEQKYSLAFV
                     GYDDESRASVLELTYNYGTDNYDLGTAYGHVAIEVDNAAAMCEAVAAKGGKVTRAAGP
                     VKGGTTVIAFVEDPDGYKIEFIERKK"
     gene            complement(1459..2160)
                     /locus_tag="AC16O9_03895"
     CDS             complement(1459..2160)
                     /locus_tag="AC16O9_03895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6515792.1"
                     /translation="MTLLRSALFALALVVVTPPYALIALATFPLPRLVRYRVISGWSR
                     LVVRLARAIVGIRWTVEGREHLPANPAVILAKHQSAWETLAFQEIFPPQVLVLKKELL
                     WIPFFGWGLALMSPIAIDRANGRAALRDIARRGRERLAQGFWVVIFPEGTRVRVGEKR
                     EYQQGGAWLAAQCGVPVVPVAHNAGRLWPRNAFVKRAGNVTVRIGPPIPTEGRDAKQI
                     SAQAEAWIEGEQRTL"
     gene            complement(2157..2690)
                     /gene="gmhB"
                     /locus_tag="AC16O9_03900"
     CDS             complement(2157..2690)
                     /gene="gmhB"
                     /locus_tag="AC16O9_03900"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR6515793.1"
                     /translation="MKLVVLDRDGTINHDSDQYIKSPAEWKPIKGSLEAIARLTQSGH
                     RVVVATNQSGIARGLFDMTTLNAIHDAMQRAVQQAGGRIDAIFFCPHAGDADCACRKP
                     RPGMLLEIARRMNAPLEGVPVVGDALRDLQAAAAVGARPVLVLTGKGKQTRAAGGLPP
                     GTEVFDNLAAFAAKAAP"
     gene            complement(2687..4759)
                     /gene="glyS"
                     /locus_tag="AC16O9_03905"
     CDS             complement(2687..4759)
                     /gene="glyS"
                     /locus_tag="AC16O9_03905"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR6515794.1"
                     /translation="MARKPAVAPRSETLLVELLTEELPPKSLEALARGFRDNLTNDLE
                     SAGLRGTGSSARYFATPRRLAVSISRVLAVAPEVRERVQGPSLKAAPEAVAGFARKHG
                     VAPDDLTRFESTRGFIYMVERTVPGAKLAEVLAHKVAAALAALPIPKTMRWGEGEAQF
                     VRPVHALVMLHGAKVMPGAVFGLEAGRRTRGHRFMSKGWITLRDADAYEAQLRDDGTV
                     LADFEVRRAEIERLLLAEAAVQRATLGEYRDLLDEVTALVEHPSVYAGAFDRAFLAIP
                     QECLALTLRQNQKYFPLFDGRGALLPKFLIVSNMRVADPRHIVGGNERVVRPRLEDAR
                     FFLEQDRKHRLEERVPQLARVVFHHKLGTQLDRVQRMKILAGMIARRLGADDALAERA
                     AELSKADLLTGMVGEFPELQGVMGRHYALQDGEAPEVAEAIGAHYRPRFAGDELPRSP
                     VGCAVALADKLYQLAGFFGIGQQPTGEKDPYGLRRAALGVIRILVERALPASLHDLVH
                     DAYQVLPKDIGQSHVAVQQFMRERMRSYLAERGYGANEIESVLGHMFVRLDQIPKQLD
                     AVRAFARLPEAPSLAAANKRIANILKQADGVPAEFDASLFSLPEESALADAFAALLPQ
                     YESAFKAGDYTGALKSLAALKSPVDAFFDKVLVMDKDERVKRNRIGFLSRLHATMNRI
                     ADLSKLAA"
     gene            complement(4764..5642)
                     /locus_tag="AC16O9_03910"
     CDS             complement(4764..5642)
                     /locus_tag="AC16O9_03910"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR6515795.1"
                     /translation="MLTFQQLIQRLSKFWDDQGCVLLQPYDMEMGAGTFHTATFLRAI
                     GPEPWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVALKPSPADIQERYLASLAALGI
                     DALEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQEVGSLPCKPVLGEITY
                     GLERLAMYLQGKESVYDLVWTPGVSYGDIYHQNEVEQSRYNFELANTELLTRHFGEFE
                     AEAKRLIEAQCTLPAYERVLQCSHAFNLLDARGAISVTERAAYIARVRALARAVAQAY
                     HESREKLGFPLTLGRR"
     gene            complement(5656..7119)
                     /gene="lnt"
                     /locus_tag="AC16O9_03915"
     CDS             complement(5656..7119)
                     /gene="lnt"
                     /locus_tag="AC16O9_03915"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR6515796.1"
                     /translation="MKRRRPLVAFLAGAAMVLAFSPFDLLPVALIALAVLTHLWVTAR
                     SARAAAGIGFVFGLGMFLAGVSWVYVSLHRFGAMPAPLAAFATLGFCAILALYPALAG
                     WAQAKLRATPALRAALLIPAAWTLAEALRATLFTGFPWLTLGSAAVGTPLAGFSPLGG
                     AYGASFATAVGAGLLWCVALGQARWRAAVAFAVIGVAGAVLRDVPWTAPTGAPFAASV
                     LQGNVPQNLKFDPLQYARTLDTYARLAEGSRARLIVLPETAVPRMLDSVDPAYLARLE
                     AAAKNNDGDLLLGVPVRVAPGEYYNAVLSLGVSPRQLYYKSHLVPFGEFVPPGFGWVV
                     RVLSIPLADFSRGPARPRPLEVAGQRVGVNICYEDAYGAELIRQLPAATLLVNVSNVA
                     WFGDSLAPAQHLQIARARALETGRMHLAATNTGLTAAIDRDGRVLARLAPFAEGRLDV
                     EVQGYAGATPYVRFGDWPALALCALVLGLVALRMRSR"
     gene            complement(7116..7937)
                     /locus_tag="AC16O9_03920"
     CDS             complement(7116..7937)
                     /locus_tag="AC16O9_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR6515797.1"
                     /translation="MNDPSRPSLLERLSSLILREPEDRAQLLALLRSAYQRNLLDADA
                     LTMIEGALTVSEMPVRDIMIPRAQMDVIDIHQKVEDFIPQVISTAHSRFPVIDQNRDD
                     VIGILLAKDLLRHYAGEEEFNVREMLRPAVFVPESKRLNVLLREFRASRNHMAIVVDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDEASDNILLESSGRYRVKALTQIADFNAAFG
                     TRFSDQEHDTVAGLVIAQLGRVPRRGETLAVDGLKIQVLRADSRRVYTLLVDKPK"
     gene            complement(7948..8349)
                     /gene="ybeY"
                     /locus_tag="AC16O9_03925"
     CDS             complement(7948..8349)
                     /gene="ybeY"
                     /locus_tag="AC16O9_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR6515798.1"
                     /translation="MQRAARGAPSAARLRAWARAAAAPGTEVTLRVVGAAEARLLNRA
                     FRKRDTATNVLSFAYSKRHGDVVLCHPVIAREARAQGKTLAAHYAHLVVHGMLHLRGL
                     DHARAADARRMERRETMLLARLGFGDPYTVE"
     gene            complement(8357..9319)
                     /locus_tag="AC16O9_03930"
     CDS             complement(8357..9319)
                     /locus_tag="AC16O9_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6515799.1"
                     /translation="MPSKPVEVRLDPVDNLRLARLCGALDANLRQIESALDVSIARRG
                     ARFTVRGEPRQAERAARALEHFYDKAAAELSLDDVQLGLTELAQGKPPEPPEPGPALL
                     TRRADLHGRTPRQVQYIEHILAHDITFGIGPAGTGKTYLAVACAVDALERDKVKRIVL
                     VRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFEKVAKLLERGTIELAPLAY
                     MRGRTLNHAFVILDEAQNTTPEQMKMFLTRIGFGAKAVVNGDVTQIDLARGQKSGLIE
                     SRRILAEVRGIAFTDFTAADVVRHPLVARIIDAYEKYSEAPPTK"
     gene            complement(9312..10637)
                     /gene="miaB"
                     /locus_tag="AC16O9_03935"
     CDS             complement(9312..10637)
                     /gene="miaB"
                     /locus_tag="AC16O9_03935"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR6515800.1"
                     /translation="MSGKLFLRTYGCQMNEVDSAMIAGVLAEAEGLAPAAQPDEADVI
                     VFNTCSVREKAQEKVFADLGRVKHLKRDNPRLMIAVGGCVASQEGAGIVARAPFVDVV
                     FGPQTLHRLPELLARRRASGAAQVDVSFPEIEKFDRLPEPPAAAASAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFEDLMGEIAALAGRGVREVTLLGQNVNAWRGEIGGAPADFA
                     ELLRYVAEIDGIGRIRYTTSHPREFTQRLIDVHAELPQLAPHVHLPVQSGADRVLAAM
                     KRGYTALEYRSIVRRLKKARPEVSLTSDFIVGFPGETEADFAATLALARELGFDGSFS
                     FLYSPRPGTPAAELDDATPREHKLERLQRLQALLDAQARAVSEAMVGGLERVLVEGPS
                     RKDAGELAGRTGNNRVVNVGAAPQLIGRFADVRITAARAHSLRGERLDA"
     gene            complement(10634..10933)
                     /locus_tag="AC16O9_03940"
     CDS             complement(10634..10933)
                     /locus_tag="AC16O9_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515801.1"
                     /translation="MTRSLVVAALIVCLAAPAHAQLRTLPAHAKPGKIRHVQEMVVQI
                     DGKPAVLARGAQVRDAHNRIVLPAAIPAGSLVRYTLNAQGEVAAVWILSAQEALK"
BASE COUNT         1606 a         3779 c         4009 g         1592 t
ORIGIN      
        1 gctgctgttc caccttgcgc gcttcgcgga ccggttgcgc gacctgcatt tcatgctgca
       61 acgcgaggtg gtggagcgca tggtcgcgcc gccctcgacg gcggcctacg ggcggctctc
      121 ggtgatgctg caggcacgtt tcaccatgca gaagctgttc cgcgtcgccg ccggcgcgtt
      181 tcgcccgccg ccgcaagtgg aatcggcggt ggtgcggctg gtccccctcg ccgagccgct
      241 cgaccgggac gtcgagggct tcgccgagct cgtgcgccgg gcgttcagtg cccggcgcaa
      301 gaccctgcgc aatgcgctcg ggctgcccgc tgaggcgctc gaggcgctcg gcatcgacgc
      361 ccggctgcgg ccggagaatc tgtccccggc cgactacgtg cgcattgcgc ggcgcaccat
      421 ttcgacactt tgttgagagc cggcaggcgt gccgagtaca atccgctcgc acccagactc
      481 gaggagacgc tcagatgaaa gccggacatg tgctcgcgct acttacaacg gccgccctgg
      541 cgggctgcca gacgatgtcg gacgagccgc tgcgcgcgtc ggcgggcttg gagccgacca
      601 agggcaacaa ggtagtcggc gaggtgaatt tcgagcaggc cggcggcaag gtgcgcgtca
      661 ccgcgcagct cattcgcctg gcgccgaacc aggagttcgg ctttcacatc cacgaggtgg
      721 gcgattgcag ctcgggcgac ggcttgagcg ccaaggggca cttcaatccc tatggcaagc
      781 cgcacgggcc gcagggcagc gagcaccatg cgggcgacat gccgaatctc aaggccgacg
      841 ccaagggcaa ggcgaagctg agcgccgatc ttgacatcgt cacgctgacc gcgggcccgg
      901 cgaacatcgt cgggcgcgcg gtgatcgtgc acgccgatcc cgacgactac aagacgcaac
      961 ccacgggcaa tgcgggcgcg cgcctggcgt gcggggtgat caaggcgaac tgagcgggtt
     1021 cgggactaaa ggaaaaaggg gcgccgctgg cgcccttttc tatttcttgc gctcgataaa
     1081 ctcgatcttg tagccgtcgg gatcctcgac gaaggcgatc accgtggtgc cgcccttcac
     1141 cggtcccgcc gcgcgcgtga ccttgccgcc cttcgcggcc acggcctcgc acatggccgc
     1201 ggcgttgtcc acctcgatgg cgacgtggcc gtaggccgtg cccaggtcgt agttgtcggt
     1261 gccgtagttg taggtgagct cgagcaccga ggcccgcgac tcgtcgtcgt agccgacgaa
     1321 ggcgagcgag tacttctgct cgggacgatc ggtggtgcgc agcaccttca tcccgagcac
     1381 ctccgtatag aacttcacgg agcgctccat gtttccgacg cgcagcatgg tgtggaggat
     1441 tcgcatggtg ccggcagtct acaacgtccg ctgctcgccc tcgatccagg cctcggcctg
     1501 cgcgctgatc tgtttcgcat cgcggccttc ggtcgggatc ggcgggccga tgcgcacggt
     1561 aacgttgccg gcgcgcttga cgaaggcgtt gcgcggccac aggcgcccgg cgttgtgggc
     1621 caccggcacc acgggcacgc cgcactgcgc cgcgagccag gcgccgccct gctggtactc
     1681 gcgcttctcg ccgacgcgca cccgcgtgcc ctcgggaaag atcaccaccc agaagccctg
     1741 cgccaggcgc tcgcggccgc ggcgcgcgat gtcgcggagc gccgcgcggc cgttggcgcg
     1801 gtcgatggcg atcgggctca tcagcgcgag cccccagccg aagaacggga tccacagcag
     1861 ctccttcttc agcaccagca cctgcggcgg gaagatctcc tggaaggcga gcgtctccca
     1921 ggccgactgg tgcttggcga ggatcaccgc ggggttggcg ggcaggtgct cgcgcccttc
     1981 cacggtccag cggatgccga caatcgcgcg cgcaaggcgc accaccaggc gcgaccagcc
     2041 ggagatgacg cggtagcgca cgaggcgcgg caggggaaag gtggcgagcg caatgagcgc
     2101 gtagggcggg gtcacgacca ccagcgccag ggcgaacagc gccgagcgca ggagcgtcac
     2161 ggcgcggcct tggcggcgaa cgcggcgagg ttgtcgaaca cctcggtgcc gggcggcagg
     2221 ccgccggcgg cgcgcgtttg cttgcccttg cccgtcagca ccaggaccgg ccgcgcgccg
     2281 accgcggccg ccgcctgcag gtcgcgcagc gcatcgccca ccaccggcac gccttccagc
     2341 ggcgcgttca tgcgccgcgc gatctcgagc agcatgccgg gcctgggctt gcggcaggcg
     2401 cagtccgcat cgcccgcgtg cgggcagaag aagatcgcgt cgatgcgccc gcccgcctgc
     2461 tgcaccgcgc gctgcatggc gtcgtggatg gcgttgagcg tggtcatgtc gaacaggccg
     2521 cgcgcgatcc ccgactggtt ggtggccacc acgacgcgat ggcccgactg ggtaaggcgc
     2581 gcgatcgcct ccaggctgcc cttgatcggc ttccattccg cgggcgactt gatgtactgg
     2641 tcgctgtcgt gattgatggt gccgtcgcgg tccaacacca cgagcttcat gcggcgagct
     2701 tcgacaggtc ggcgatgcgg ttcatggtgg cgtgcagccg gctgagaaag ccgatgcggt
     2761 tgcgcttcac gcgctcgtcc ttgtccatca ccagcacctt gtcgaagaac gcgtcgacgg
     2821 gggacttgag cgcggcgagc gatttcagcg cgccggtgta gtcgcccgcc ttgaaggcgc
     2881 tctcgtactg cggcagcagg gcggcgaagg cgtcggcgag ggcgctttcc tcgggcaggc
     2941 tgaacagcga cgcgtcgaac tccgccggca cgccgtcggc ctgcttgagt atgttggcga
     3001 tgcgcttgtt ggccgccgcg aggctggggg cctcgggcag cctggcgaac gcgcgcacgg
     3061 cgtcgagctg cttcggaatc tggtcgaggc gcacgaacat gtgacccagc accgactcga
     3121 tctcgttcgc gccgtaaccg cgctcggcga gatagctgcg catgcgctcg cgcatgaact
     3181 gctgcaccgc cacgtgcgac tggccgatgt ccttgggcag cacctggtag gcgtcgtgca
     3241 ccaggtcgtg cagcgacgcc ggcagcgcgc gctcgaccag gatgcggatc acgccgagcg
     3301 ccgcgcggcg caggccgtag ggatccttct cgccggtggg ctgctggccg atgccgaaga
     3361 atcccgccag ctggtagagc ttgtcggcga gcgccactgc gcagcccacc gggctgcgcg
     3421 gcagctcgtc gccggcgaag cgcgggcggt agtgcgcgcc gatcgcctcg gccacctcgg
     3481 gcgcctcgcc gtcctgcagc gcgtagtggc gtcccatgac gccctgcagc tcgggaaact
     3541 cgcccaccat gccggtcagc aggtccgcct tggagagctc ggcggcgcgc tcggccaggg
     3601 cgtcgtccgc gccgagccgg cgcgcgatca tgccggcgag gatcttcatg cgctgcacgc
     3661 gatcgagctg ggtgccgagc ttgtggtgga acaccacccg ggcgagctgc ggcacccgct
     3721 cctcgagccg gtgcttgcgg tcctgctcga ggaagaagcg cgcgtcctcg aggcgcgggc
     3781 gcaccacgcg ctcgttgccg ccaacgatgt ggcggggatc cgcgacgcgc atgttggaga
     3841 cgatgaggaa tttcggcagc agcgcgccgc gcccatcgaa cagggggaag tacttctggt
     3901 tctggcgcag cgtgagcgcg aggcactcct gcgggatcgc caggaacgcc cggtcgaagg
     3961 cgccggcgta gacgctcggg tgctcgacca gggcggtgac ctcgtcgagc aggtcgcggt
     4021 actcgccgag cgtggcgcgc tggacggcgg cctcggcaag gagcagccgc tcgatctccg
     4081 cgcggcgcac ctcgaagtcg gccagcaccg tgccgtcgtc gcgcagttgc gcctcgtagg
     4141 cgtcggcgtc gcgcagcgtg atccagccct tggacatgaa gcggtggccg cgcgtgcgcc
     4201 ggcccgcctc caggccgaac acggcccccg gcatgacctt ggccccgtgc agcatgacca
     4261 gcgcgtgcac cgggcgcacg aactgcgcct cgccctcgcc ccagcgcatc gtcttcggga
     4321 tcggcagcgc cgcgagcgcg gcagcgacct tgtgcgccag gacctcggca agcttcgcgc
     4381 cgggcacggt gcgctcgacc atgtagatga agcccctggt ggactcgaag cgcgtgagat
     4441 cgtcgggcgc gacgccatgc ttgcgcgcga acccggcgac cgcctccggc gcggccttga
     4501 gcgagggtcc ctgcacgcgc tcgcgcacct cgggcgccac cgccagcacg cgcgagatgg
     4561 atacggcgag ccgccgtggc gtggcgaagt agcgcgcgct gcttcccgtg ccgcgcaggc
     4621 cggcgctctc cagatcgttg gtgagattgt cgcggaagcc gcgcgccagc gcctccagcg
     4681 acttcggcgg cagctcctcg gtcagcagct cgacgagcag cgtttccgac cgcggcgcaa
     4741 cggcgggttt gcgtgccatg gtgctagcgc cggcccaggg tcaagggaaa gccgagcttc
     4801 tcgcgcgatt cgtggtaggc ctgcgccacg gcgcgcgcca gcgcgcgcac gcgcgcgatg
     4861 tacgcggcgc gctcggtgac cgagatcgcg ccgcgcgcat cgagcaggtt gaacgcgtgc
     4921 gagcattgca gcacccgctc gtaggccggc agcgtgcact gcgcctcgat caggcgcttc
     4981 gcctcggcct cgaactcgcc gaagtggcgc gtgaggagct cggtgttggc gagctcgaag
     5041 ttgtagcgcg actgctcgac ttcgttctgg tggtagatgt cgccataaga gacgcccggc
     5101 gtccacacca ggtcgtacac ggactccttg ccctgcaggt acatcgccag gcgctccagg
     5161 ccgtaggtga tctcgccgag caccggcttg cagggcaggg acccgacttc ctggaagtag
     5221 gtgaactgcg tgacttccat gccgtcgagc cacacttccc accccaggcc ccaggcgccc
     5281 agcgtcggcg actcccagtc gtcctcgacg aagcggatgt cgtgctcgag cgcgtcgatg
     5341 ccgagcgcgg cgagcgaagc gaggtagcgc tcctggatgt ccgcgggcga gggcttcagc
     5401 gccacctggt actggtagta gtgctgcagg cggttggggt tctcgccgta gcgtccgtct
     5461 ttcggtcggc gcgagggctg cacgtaggcg gcgttccagg gctccgggcc gatggcgcgc
     5521 aggaaggtcg cggtgtggaa ggtgcccgcg cccatctcca tgtcgtaggg ctgcaacagc
     5581 acgcacccct ggtcatccca gaacttcgac aggcgctgga tcagctgctg gaacgtgagc
     5641 atgtcggacg gcatcttacc ggctgcgcat gcgcagcgcg acgaggccaa gcacgagcgc
     5701 acacagggcg agcgccggcc agtcgccgaa gcgcacgtaa ggcgtggcgc cggcgtagcc
     5761 ctgcacctcg acgtcgaggc ggccttcggc gaacggggcg aggcgcgcca gcacgcggcc
     5821 gtcgcggtcg atcgccgcgg tgaggccggt gttggtcgcg gcgaggtgca tgcgcccggt
     5881 ctcgagggcg cgcgcgcgcg caatctgcaa gtgctgcgcc ggggcgagcg agtcgccgaa
     5941 ccaggccacg ttggagacgt tgacgagcag cgtcgccgcg ggcagctggc ggatgagctc
     6001 ggcgccatag gcgtcctcgt agcagatgtt gacgccgacg cgctggccgg cgacttccag
     6061 cgggcgcggg cgcgccgggc cgcgcgagaa gtcggcgagc gggatcgaca gcacgcgcac
     6121 cacccagccg aagcccggcg gcacgaactc gccgaagggc acgaggtgcg acttgtagta
     6181 gagctggcgc ggcgacacgc cgagcgagag cacggcgttg tagtactcgc ccggcgcgac
     6241 gcgcaccggc acgccgagca gcaggtcgcc gtcgttgttc tttgccgccg cctcgagccg
     6301 cgcgagatag gcgggatcga cgctgtcgag catgcgcggc accgcggtct cgggcaggac
     6361 gatgagccgt gcgcggctgc cttccgcgag gcgggcgtag gtgtccagcg tgcgcgcgta
     6421 ctgtagcgga tcgaacttca ggttttgcgg cacgttgccc tgcagcacgc tggcggcgaa
     6481 cggcgcgccg gtgggcgccg tccagggcac gtcgcgcagc acggcgccgg cgacgccgat
     6541 gacggcgaag gccacggcgg cgcgccagcg cgcctggccg agcgccacgc accacagcag
     6601 gcccgcccct accgccgtcg cgaacgacgc gccataggcg ccgccaaggg gcgagaagcc
     6661 cgcaagcggt gtgccgacgg cggcgctgcc gagcgtgagc cagggaaacc ccgtgaacag
     6721 cgtcgcgcgc aaggcttcgg cgagcgtcca ggccgccggg atcaggagcg cggcgcgcag
     6781 cgccggcgtt gcgcgcagct tcgcctgcgc ccagccggcg agcgccggat acagggcgag
     6841 gatggcgcaa aagccgagcg tggcgaaagc ggcgagcggc gccggcatgg cgccgaagcg
     6901 gtgcaggctc acgtagaccc acgatacgcc cgcgagaaac atgcccaggc cgaacacgaa
     6961 gccgatgccg gcggcggcgc gcgcgctgcg cgccgtgacc cagaggtggg tgagcaccgc
     7021 aagcgcgatc agcgcgaccg gcagcaggtc gaacggggaa aaggccagca ccatcgccgc
     7081 gccggcgagg aacgcgacga gggggcggcg gcgcttcact tcggcttgtc gacgagcagc
     7141 gtgtacacgc gccggctgtc ggcgcgcagc acctggatct tgaggccgtc cacggcgagc
     7201 gtctcgccgc ggcgcggcac gcggccgagc tgcgcgatca ccagcccggc cacggtgtcg
     7261 tgctcctggt cggaaaagcg cgtgccgaag gcggcgttga agtcggcgat ctgggtgagc
     7321 gccttgacgc ggtagcgccc gctcgattcg agcaggatgt tgtcgctcgc ctcgtcgaag
     7381 tcgtactcgt cctctatgtc gccgacgatc tgctccagca cgtcctcgat ggtcacgagg
     7441 cccgcgacgc cgccgtactc gtcgaccacg atggccatgt ggttgcggct ggcgcggaac
     7501 tcgcgcagca gcacgttgag gcgcttggac tcgggcacga acaccgccgg gcgcagcatc
     7561 tcgcgcacgt tgaactcctc ctcgcccgcg tagtggcgca gcaggtcctt ggcgagcagg
     7621 atgccgatca cgtcgtcgcg gttctggtcg atcaccggga agcgcgagtg cgcggtggag
     7681 atgacctgcg ggatgaagtc ctcgaccttc tggtggatgt cgatcacgtc catctgggcg
     7741 cgcgggatca tgatgtcgcg caccggcatc tccgagaccg tgagcgcgcc ctcgatcatg
     7801 gtgagggcgt cggcgtcgag gaggttcctc tggtaggccg accgcagcag cgcgagcagc
     7861 tgcgcgcggt cctcgggctc gcgcaggatg agcgaggaca ggcgctcgag caggctaggt
     7921 cgggaggggt cgttcatgcc gcgcgggtca ttctaccgtg taggggtcgc cgaacccgag
     7981 gcgcgcgagg agcatcgtct cgcggcgctc catgcgccgc gcgtccgcgg cgcgggcgtg
     8041 gtcgagcccg cgcaggtgca gcatgccgtg caccacgagg tgcgcgtagt gcgcggcaag
     8101 cgtcttgccc tgcgcgcgcg cttcgcgcgc gatcaccggg tggcacagga ccacgtcgcc
     8161 gtggcgcttc gagtacgcga acgacagcac gttggtggcg gtgtcgcgct tgcggaacgc
     8221 gcggttgaga agccgcgcct cggcggcgcc gacgacgcgc agcgtcactt cggtgccggg
     8281 ggcggcggcg gcacgcgccc aggcgcgcag tcgcgccgcg gacggcgcac cgcgcgccgc
     8341 gcgctgcacg gcgatgctac ttcgtgggcg gcgcttcgga gtacttttcg tacgcatcga
     8401 tgatgcgcgc gacgagcggg tggcgcacga cgtccgccgc ggtgaagtcg gtgaaggcga
     8461 tgccgcgcac ctcggcgagg atgcggcgcg attcgatgag gccgctcttt tgcccgcgcg
     8521 ccaggtcgat ctgcgtcacg tcgccgttca ccaccgcctt ggcgccgaag ccgatgcgcg
     8581 tgaggaacat cttcatctgc tcgggcgtcg tgttctgcgc ctcgtcgagg atgacgaagg
     8641 cgtggttcag cgtgcgcccg cgcatgtagg cgaggggcgc cagctcgatg gtgccgcgct
     8701 cgagcagctt ggccaccttc tcgaagccca tcaggtcgta gagcgcgtcg tacagcggcc
     8761 gcaggtacgg atcgaccttc tgcgccaggt cccccggcag gaagccgagg cgctcgccgg
     8821 cttcgaccgc gggccgcacc aggacgatgc gcttcacctt gtcgcgctcg agcgcgtcca
     8881 ccgcgcaggc caccgccagg taggtcttgc cggtgcccgc cgggccgatg ccgaaggtga
     8941 tgtcgtgcgc gaggatgtgc tcgatgtact gcacctggcg cggcgtgcgc ccgtgcaggt
     9001 cggcgcgccg cgtgagcagc gccggcccgg gctcgggcgg ctcgggcggc ttgccctgcg
     9061 cgagctccgt gagcccgagc tgcacgtcgt ccaggctcag ctcggccgca gccttgtcgt
     9121 agaagtgctc cagcgcgcgc gccgcgcgct cggcctgccg cggctcgccg cgcaccgtga
     9181 agcgcgcgcc gcgccgcgcg atcgacacgt cgagcgccga ctcgatctgg cgcaggttgg
     9241 catccagcgc gccgcacagg cgcgccagac gcaggttgtc caccggatcg agacggactt
     9301 cgaccggctt gctaggcatc caggcgctcg ccgcgcagcg aatgcgcgcg cgcggccgtg
     9361 atgcgcacgt cggcgaagcg cccgatgagc tgcggcgcgg cgccgacgtt caccacgcgg
     9421 ttgttgccgg tgcgcccggc gagctcgccc gcgtccttcc tcgacgggcc ctcgacgagc
     9481 acgcgctcga ggccgcccac catggcttcg ctcaccgcgc gcgcctgcgc gtcgagcagc
     9541 gcctgcagcc gctgcaagcg ctcgagcttg tgttcccgcg gcgtcgcatc gtcgagctcg
     9601 gccgcgggcg tgccgggacg cgggctgtag aggaaagaga acgagccgtc gaagccgagc
     9661 tcgcgtgcga gcgcgagcgt ggcggcgaaa tcggcctcgg tctcgccggg gaagccgacg
     9721 atgaagtccg aggtgaggct cacctccggc cgcgccttct tcagccggcg cacgatcgag
     9781 cggtactcga gcgcggtata gccgcgcttc atggcggcga gcacgcggtc ggcgcccgat
     9841 tgcaccggca ggtgcacgtg cggtgcgagc tgcggcagct ccgcatgcac gtcgatcagg
     9901 cgctgcgtga actcgcgcgg gtgcgacgtg gtgtagcgaa tgcggccgat gccgtcgatc
     9961 tcggccacgt agcgcaggag ctccgcgaag tcggccggcg caccgccgat ctcgccgcgc
    10021 caggcgttca cgttctggcc gaggagcgtc acctcgcgca cgccgcgtcc ggcgagcgcg
    10081 gcaatctcgc ccatcaggtc ctcgaacggc cgcgagattt cctcgccgcg cgtatagggc
    10141 accacgcaga agctgcaata cttgctgcag ccctccatga tggagacgaa ggcgctcgcc
    10201 gcggcggccg gcggctcggg caggcggtcg aacttctcga tctcggggaa gctgacgtcc
    10261 acctgcgccg cgccgctcgc gcgccggcgc gcgaggagct cgggcaggcg gtgcagcgtc
    10321 tgcgggccga acaccacgtc cacgaacggc gcacgcgcga cgatgcccgc gccctcctgg
    10381 ctcgcgacgc agccgcccac ggcgatcatc aggcgcgggt tgtcgcgctt gaggtgcttc
    10441 acgcgcccga ggtcggcgaa caccttctcc tgcgccttct cgcgcaccga gcaggtgttg
    10501 aacacgatga cgtcggcctc gtcgggctgc gccgcgggcg cgagcccttc ggcctcggcc
    10561 agcaccccgg cgatcatcgc cgagtcgacc tcgttcatct ggcagccgta ggtgcgaagg
    10621 aacagcttgc cgctcacttg agcgcctcct gcgcgctgag gatccacacc gcggccactt
    10681 caccctgcgc gttcagcgtg tacctgacca ggctgcccgc gggaatcgcg gcgggcagca
    10741 caatgcggtt gtgggcgtcg cgcacctgcg cgccgcgcgc gagcactgcc ggcttgccgt
    10801 cgatctgcac caccatctcc tgcacgtggc ggatcttgcc gggcttggcg tgcgcgggca
    10861 gcgtgcgcag ctgcgcgtgc gccggtgcgg caaggcagac aatgagggcg gcgacgacca
    10921 gcgaacgcgt catcggagcg cggcgggagg acagtgatga caggcggcca ttgtatcaag
    10981 gggccg
//
LOCUS       DBNHKE010000062        40648 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_909779, whole genome shotgun sequence.
ACCESSION   DBNHKE010000062 DBNHKE010000000
VERSION     DBNHKE010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 40648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 40648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_909779"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..2004
                     /gene="dxs"
                     /locus_tag="AC16O9_03945"
     CDS             145..2004
                     /gene="dxs"
                     /locus_tag="AC16O9_03945"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR6515802.1"
                     /translation="MTSDPRFPLLSAIDDPHDLRDLPESKLPAVAAELREFLVDSVGK
                     TGGHLASSLGTVELTVALHYVYQTPDDRLLWDVGHQAYGHKVLTGRRKLMHTLRQGGG
                     LSGFLKRSESPYDAFGAGHSSTSISAALGMAIASKRQGKPHRVAAIIGDGALTAGMAF
                     EALNHGGDLDADLLVVLNDNDMSISPNVGGLSNYLAKLLSGPVYTGMREGGKRVLSHL
                     PPFQELARRAEEHLKGMVMPGTLFEELGFNYIGPIDGHDLDTLIATLTNIKKLKGPQF
                     LHIVTRKGQGYKLAEDDPIAYHGPSKFDPAEGIKKAAAPAKPTYSQVFGDWLCDMAAA
                     DARLMAITPAMREGSGMVQYSEKFPERYFDVGIAEQHSVSFAAGLACEGLRPVVAIYS
                     TFLQRGYDQLIHDVAIQNLPVTFALDRGGLVGGDGATHNGAFDFAYLRTVPNLTVMAP
                     ADAQECRRMLSTAFHLGAPAAVRYPRGAALGEAGSALDTLPVGKGQLRRSGQRVAILA
                     FGSMLKPALEAAEALDATVANMRFVKPLDTELLRELAQGHELLVTVEEHQIMGGAGSA
                     VCEALAGLRIEKPVLLLGLPDRFIDHGDPAKLLSSVGLDAPGIETAIRALISE"
     gene            2030..2833
                     /gene="folE2"
                     /locus_tag="AC16O9_03950"
     CDS             2030..2833
                     /gene="folE2"
                     /locus_tag="AC16O9_03950"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6515803.1"
                     /translation="MKAKDLHNIPDVQSTPDSRKLAIDKVGIKAIRHPIQIMQRAGGV
                     QHTIANFNMYVGLPHHFKGTHMSRFVEILSAHEREITVDSFQAMVKEMVAKLEAEEGV
                     VEMTFPYFIEKAAPVSRVKSLMDYEVSFVSQILDGRQGFAMKVLVPVTSLCPCSKKIS
                     AYGAHNQRSHVTVTAHTGEFVWIEEIVDLVEKQASSELYGLLKRPDEKHVTERAYDNP
                     KFVEDMVRDVAALLNLDERITRFVVESENFESIHNHSAYALIERDKTAG"
     gene            2838..3539
                     /locus_tag="AC16O9_03955"
     CDS             2838..3539
                     /locus_tag="AC16O9_03955"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6515804.1"
                     /translation="MLAGLGISAVGAQDFDARRMRLIDEIDAMYAATRAETGLATMSP
                     AVRAAMARVERHRFVPAAERDRAYGNYPLPIGAGQTISQPYIVALSTDLVAPRPGHAV
                     LEIGTGSGYQAAVLAEIVAQVYSIELIPDLGRAAAARLAAAGYGNVEVRIGDGYAGWP
                     EKAPFDGIVVTAAAPRVPQALVDQLKPGARLVIPVGATHAVQELLVITKRADGGTDER
                     RVLPVRFVPLVPGRR"
     gene            complement(3536..4192)
                     /locus_tag="AC16O9_03960"
     CDS             complement(3536..4192)
                     /locus_tag="AC16O9_03960"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR6515805.1"
                     /translation="MAIGFMWRHCSAALLAVLALAPAAAHALDVRLVGTFGDKAAILV
                     IDGGGPRTVRIGREVAGVTVVSVERDSAVIEVAGTQRTLARGQHYSTGAGDARQSVTL
                     AADTRGHFITEGAINGRAVRFVIDTGATMVALPAADALRLGLDYRKGEVALTKTAAGV
                     VPVYRVRLDSVRLGAIEINAVDGVVIEQGLDIALLGMSFLSRVEMKNEGQLMTLTRRY
                     "
     gene            4176..4889
                     /locus_tag="AC16O9_03965"
     CDS             4176..4889
                     /locus_tag="AC16O9_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR6515806.1"
                     /translation="MKPIAIFRHAPSEGPGYFASYLERKAIAWRLVPLDAGHAVPRST
                     RGFSGLVFMGGPMSANDELPWIIPALSLIADAVRNDVPVLGHCLGGQLMARAFGGEVR
                     AAPVTEIGWGEVAVADNAVAREWMGAIEAFESFHWHGETFSIPPGGTRVLENAHCANQ
                     AFALGRHFGMQCHVEMTPEMIAEWVRGGAAEIAQSRDSPGVQAPDEIERNVERRTAAL
                     HAVADRIYDRWCEGLSRGS"
     gene            complement(4873..5358)
                     /locus_tag="AC16O9_03970"
     CDS             complement(4873..5358)
                     /locus_tag="AC16O9_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR6515807.1"
                     /translation="MPSFDIVSQVNAQEVKNAVEQANKEIQNRFDFKGSDARIEQAEM
                     ALTVFADDEYKLGQVIDVLRQRMAKRSIDVRCLELGAVEKAAGDKAKRVVTVKVGIAQ
                     EKAKQIQKAIKDAKLKVQASIQGDLVRVSGGKKDDLQAAIQLVRQSVTDQPLQYINFR
                     D"
     gene            complement(5370..7562)
                     /locus_tag="AC16O9_03975"
     CDS             complement(5370..7562)
                     /locus_tag="AC16O9_03975"
                     /inference="COORDINATES: protein motif:HMM:NF018047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HIR6515808.1"
                     /translation="MRASAAAPTVAGAYTGEIAAPYRAPELLAQLARLPVLLEAPGAR
                     LVAEGRNRNIVVELPLQDGARGVMVKAFGVQPWFRDLRDRGRGSKAARTWRAATHLAA
                     QGAGTPPPIGYLERWDGARLRESYYLAEYQDGAQTLREALLALYDARPPLAAHFVALL
                     ERVADGMRGMHAAGFVHNDLGAQNVLLRPAGEAGWRDFMVVDLNRGRLRGELTLRERG
                     RDFSRLELPSDLLQMLIEMYWRGVPPRELLDWQRRYRERYAWKVRLRRVRSALRGRRP
                     DPAARDYPAPRDVWIWDEDTAQPISALLRRDRVRHFPRSRYTRVLVDALRDAPRAWRE
                     YRGLLPLAYTQPVGLGGRVGMALEPTPQTLEGELALLAGLGPLPALVRFYHHDARETR
                     RWRAALVHALHRAGHAVSVALVQDRRAVCEPESWRRFAFEALEDVAGVVEAVEVGHAV
                     NRVKWGIWGFEDLRALYAPLAELQARFPALRFMGPALIDFEYPIAFPVLREWPAGVPL
                     AALSHHLYVDRRGAPENPQNGFGAAEKFTLGRALARAAPQCADRFVVSEVNWPLRGTG
                     AHAPIHAPYYPPWRHASDPGTSEDEYADYLLRYLCLALGSGMVERVYWWRLAARGFGL
                     VDDADPAALRPRPAYAMLQHFLRLLGASTFIGARLPGRRGERHGRYRFAFRRGDAEIV
                     ALTYAHGPALAFPADEAFERAEDAFGRPLAAAPDRLSGRPVYLRGARA"
     gene            complement(7552..8208)
                     /locus_tag="AC16O9_03980"
     CDS             complement(7552..8208)
                     /locus_tag="AC16O9_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515809.1"
                     /translation="MRALAISLLAVLWLDCAAEPFAARVGDMRVVLDTPPGFADTLDL
                     GSPRLQELAESLTSASNRILLFALTDADLRLFSRGDAPALHRYLLLATPRALERDSVG
                     LSQFRDYAVESARSLGEAAQPQDIRAFLSAQALGRANVLRVLRSEPLAVSRLMGTRLP
                     PRYGDTPEYLLYTTTQLLLRGKVLSVSAYTGLRDAADTAWLLDITQRWTSELQRLNAR
                     "
     gene            8264..9607
                     /gene="argG"
                     /locus_tag="AC16O9_03985"
     CDS             8264..9607
                     /gene="argG"
                     /locus_tag="AC16O9_03985"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR6515810.1"
                     /translation="MATILSTLPTGERVGIAFSGGLDTSAAVHWMRAKGAVPYAYTAN
                     LGQPDESDYGAIPKKALACGAEVARLVECRPQLVAEGIAAIQCGAFHISTGGATYFNT
                     TPLGRAVTGTMLVVAMREDAVGIWSDGSTYKGNDIERFHRYGLLANPSLRIYKPWLDQ
                     RFIDELGGRKEMSAYMAQAGFDSKVSADKAYSTDSNLLGATHEAKDLEFLDRGLGIVE
                     PIMGVAFWREGVKVKPEPVSVRFEAGVPVALNGQQFTDAVALMAEANAIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRTNGRRLGRLLYQG
                     RWFDPQTMMLRETAQRWVAKAVTGEVALELRRGNDWSVLDTRSPNLTYKPERLTMEKG
                     EAFFTPADRIGQLTMRNLDIADTRDKLRVYGEAGLLGESKDGGVPLLGPEKPGKR"
     gene            complement(9604..11043)
                     /gene="fdrA"
                     /locus_tag="AC16O9_03990"
     CDS             complement(9604..11043)
                     /gene="fdrA"
                     /locus_tag="AC16O9_03990"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HIR6515811.1"
                     /translation="MSVVNRLRRGFYADSVVLMRITRELNKGGVTASLMIGTPSNKAL
                     LKASGLLTKAGDAAGPDDLIIAVRGPKAQAALALAEKLLQKGTQTRAAGESFPTLASA
                     IAGLAGANLALISVPGEYAAAEARRALEHGLHVMMFSDNVPLADEVALKDLALERGLL
                     MMGPDCGTALLGGAPIGFANAVSRGDVGIVSASGTGLQEVSTLLARQGKGVSHGIGVG
                     GRDLSEAVGARMTLAALDALEADPATRRIIVISKPPAPAVVKRIVARAGRSRKPVTLC
                     LLGADELAVPRGVSLARTLEQASGKPARGAPPRLAAKGNIRGLFCGGTLCAEAQIVLM
                     DHGLDVSSNVPVPGAAPARAGHLLIDLGADDYTRGRPHPMIEPELRNAYLARALRDKE
                     VGLVLLDFVLGHGAHPDPAGLAAALLKNKSKPVIASVTGTDGDPQVSSRQARILEDAG
                     VVVAASNAQAARFAAAALRGAPGRGRARR"
     gene            complement(11040..12479)
                     /locus_tag="AC16O9_03995"
     CDS             complement(11040..12479)
                     /locus_tag="AC16O9_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbE family protein"
                     /protein_id="HIR6515812.1"
                     /translation="MSRPSSPIASLLARPPRVVNVGLRAFAAELAGRDVAVAHVEWRP
                     PAAGREHAAALLGALARHEARIAQANAEGLRRMLSAEPALVDVLPAGEAMPGLDRHVV
                     LHAGPPIDWPRMCGPMRGAVAGALVFEGWAPDLAAATRLAASGAIDFRPNHALGAVGP
                     MTGITTRSMPVMVVENRAFGNRSYCMINEGLGKVMRFGANDAEVLARIAWLRDEFGPR
                     LGAALRAAGGLDLAPLVARGLAMGDEMHQRNVACSALVLRGLAPHLARAAGGAEPLAQ
                     SLAFIAGNEQFFLNVGMAMAKSVMDPVRGIEGCSLVTAMCRNGTDFGIRLSATGDAWF
                     TAPVEMPQGLYFAGYGERDANPDMGDSAIMETVGLGAFAMAASPAVMGFVGAGRASDA
                     LAFTRAMGELTVGANPRWTIPALDARGVPAGIDARKVVDTGIQPAINTGIAHRRPGVG
                     QVGAGVARAPLACFDQALAALVRALGSRP"
     gene            12501..13460
                     /locus_tag="AC16O9_04000"
     CDS             12501..13460
                     /locus_tag="AC16O9_04000"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR6515813.1"
                     /translation="MPEVSTAQRLVVAIGGNATHPENIRGTTAEQEEIAARAARALRP
                     LVEAVPEIVITHGNGPVVGKIMLRQYYAREHVPPMRLDVCVAHSQGGIGHLLMQGLED
                     ELARADIERRVACIVTRVEVNEGDEAFTHSTKPVGPFFSAEEAAELKRTLGWNMVEDA
                     GRGWRYLVPSPRPRRILDLDLIAGLARQGVVVIAAGGGGIPMVRRPDGSYAGVPAVVD
                     KDLASALLAAELHYDTLLILTAVPRVLVHFRTAREHAIESVSAAELQALHREGHFAEG
                     SMGPKVRAALDFLAAGGRRAIIAHLDQAADALAGAAGTHVLPG"
     gene            13540..14193
                     /locus_tag="AC16O9_04005"
     CDS             13540..14193
                     /locus_tag="AC16O9_04005"
                     /inference="COORDINATES: protein motif:HMM:NF022834.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxamate carbamoyltransferase subunit AllH family
                     protein"
                     /protein_id="HIR6515814.1"
                     /translation="METAAGIMCLGGPGLGRGPLNVLVGAYSHYSVGDRIEIDLSSAA
                     RWRPELRLATHRSVPARIREPWRDRGEAQAFLRWIAGAGGRPNDALIGLGAGLTPAGD
                     DFVGGAMIALRAAGELALAERIAAWALSLAETRTGRISRAHLAWAAQGEGHEALHALL
                     ASNEGSARFEQSLARLTRIGHTSGLDAAAGALLALAARRGGSGLGPGLDLAEQQELD"
     gene            complement(14143..15078)
                     /gene="argF"
                     /locus_tag="AC16O9_04010"
     CDS             complement(14143..15078)
                     /gene="argF"
                     /locus_tag="AC16O9_04010"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR6515815.1"
                     /translation="MAKLRHFLQFKDLARAECEHLFARTKWIKAQFKRYRRYWPLEDR
                     TLVMIFEKQSTRTRLSFEAGMHQLGGAAIFLSTRDSQLGRGEPVEDAAQVISRMCDIV
                     MIRTFEQDIIERFAAHSRVPVINGLTNEYHPCQVLADIYTFVEQRGAIRGKTVAWIGD
                     SNNVCNTWLQAAEVLDFNVHVSTPPGYEVEPERAGLYGTGHYEEFRDPMQAAKNADLV
                     TTDVWTSMGFEAENEARKRDFEDWQVDAEMMKAAKKNALFMHCLPAHRGEEVAAEVID
                     GPASVVWDEAENRLHTQKALIEFLLLGKVKARAKA"
     gene            complement(15098..16288)
                     /locus_tag="AC16O9_04015"
     CDS             complement(15098..16288)
                     /locus_tag="AC16O9_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR6515816.1"
                     /translation="MNTYARLPVAFVRGQGVWLYDEADKKYLDALAGIAVNTLGYAHP
                     RLVRALTEQIGRVIHTSNLFQMPLQEQAADRICEITGLDEVFFCNSGLEANEAALKVA
                     RKYGHDRGIAEPAIIVMEKAFHGRSLATLSATGSRKVQAGFEPLVQGFVRVPLNDLDA
                     VRQVAERNSNVVAVFIEPIQGEGGINISRLEYLRGLKEICERNDWLFMSDEVQCGLGR
                     TGKWFVYQHAGIQPDVVPLAKGLASGVPVGACVVGGRAKGVFKPGNHGSTFGGNPLAM
                     CGVVTTLDTMKEEDLLANAARVGDAIREGLGAALAGVAGVAEVRGMGMMIGVELDRPC
                     GDIVKTALERGLVTNVTADTVIRLLPPLVMNADEGRQVVERLVPIIREFLAQSAAPRA
                     AAAR"
     gene            complement(16368..18341)
                     /locus_tag="AC16O9_04020"
     CDS             complement(16368..18341)
                     /locus_tag="AC16O9_04020"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6515817.1"
                     /translation="MAKPVSRILVINDELLVLREFVKGLNAAARSLDNPLGITFAGVT
                     TAKEALAAIQSDGDIQAVLVDDTLYTLAKHNGSRRLQVSALELVQRITRFRPELSVYI
                     LIAQEKEDDVVDALFTESVDGYFYREERDYRGIYRILNAQIQERARTPFTDQLKNYVW
                     MAKDQWHTPGHSSGESLRGSPWVNDFYEFMGEHVFDADLSVSVPMLDSLMDPTGVIAE
                     AQLMAAQAFGARRTFFATNGTSTANKVIFQTLLAPGEKLLLDRNCHKSVHHGVVLSGA
                     HPVYLDSALNRRYGLYGPVPKKTVLEAIRRHPDAEALILTSCTYDGLRYDLKPIVEAA
                     HAKKIKVIIDEAWYGFARFHPEFRPTALEAGADYATQSTHKVLSAFSQASMIHVNDPD
                     FREHLFRENFNMHTSTSPQYGLIASLDVARKQAVMEGYKLLSRTLALAKELREQINST
                     KVFRSLELEDLLPEELRHDGIRLDPTKVTVDISGCGYTVDDLQKLLFERFNIQTEKST
                     FNTLTLLLTIGTTRSKVSRLYDAFMRIAREKRAPRRLVRTPEIPAFTKLRYLPRDAYY
                     CGGELVPVFDDKERVNKSLARRVCADQVVPYPPGIPVLVPGQVITRDIAEYLASLLRS
                     QKRMEIHGVVYEGYQPCVRVLRPAEARGLHRIG"
     gene            complement(18429..18692)
                     /gene="rpsT"
                     /locus_tag="AC16O9_04025"
     CDS             complement(18429..18692)
                     /gene="rpsT"
                     /locus_tag="AC16O9_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6515818.1"
                     /translation="MANIKSSRKSARQAVVRRAHNFTLRTEVRTAIKNVKKAIAGGNK
                     AAAAAALEKSRSVIDRTAAKGILHRNAASRHMSRLAQAIKAMQ"
     gene            18731..20272
                     /gene="murJ"
                     /locus_tag="AC16O9_04030"
     CDS             18731..20272
                     /gene="murJ"
                     /locus_tag="AC16O9_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6515819.1"
                     /translation="MNLLRVLATVSSMTFLSRVLGFARDFFIARFFGAGLATDAFFVA
                     FRIPNLLRRMFAEGAFSQAFVPILSEYKNRQSAEETRSLVDVIGTLLFVMLVVATLIG
                     MLAAPLIGYLFVPRWFHAEPDKFALTVQLLRITFPYILFISLVALAAGVLNTWSRFAV
                     PAFTPVLLNVSFIVAAAFFAERFDPPVLALAWAVFIGGALQLALQLPFLARIGMLPRW
                     RLDLGHPGARRVLTLMVPAAFGVSVSQVSLLINTIFASFLVTGSVSWLYYADRLMEFP
                     AGMLGVAVGTILLPSLSRHYASGAAAEYAKLLDWGLRVTLLLALPAAAALAVLAVPLI
                     TTLFHYGRFGADDVWMTRQALVAYSLGLVGMILVKILAPGFYARQNVVTPVKIGLVTL
                     AATQLMNLAFIVPLKHAGLALAIGLGACLNAALLYRNLRRHGIYAPQPGWGAFAGRIA
                     FAVTSMSAVLYFAMGPSTWWLQAPWQHKAAGMCGLVALGVAAYGLILLALGFHWRDFV
                     RREAP"
     gene            20272..21123
                     /locus_tag="AC16O9_04035"
     CDS             20272..21123
                     /locus_tag="AC16O9_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR6515820.1"
                     /translation="MNVPDPFAELMARDEAEIDLARACLLIATDAYPGLDVEGYLGEI
                     ERLAARLRGRLAPGGGAEERVLALNQFLFDDLGYSGNADNYYDPRNSFLNEVLDRRTG
                     IPITLSVLYLEIGRRIGLELEGVSFPGHFLVRLRLRGAMLVLDPFSGGEALSESDLRE
                     RLQRVIPEGAAGGVPVGALPLDPFLEPAGKRQILARLLRNLKGIYREADKPQRLLDVL
                     NRMLVVAPEAHGELRERGLLYQKLECYRAALKDLQDYSALAPEAPDIDDVRAKLVELS
                     AQCARLN"
     gene            21250..22185
                     /locus_tag="AC16O9_04040"
     CDS             21250..22185
                     /locus_tag="AC16O9_04040"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR6515821.1"
                     /translation="MRVTHGLTAPAGKRLALTIGNFDGVHRGHQAMLARTVARARELR
                     LDACALTFEPHPREFFTPATAPARLTRLRDKLELMAAAGLDRVHVARFDARLASLPAE
                     RFVDTYVAHGLRTAWLLVGRDFRFGARRGGDFALLGELAGRHGFELEAMPEVALHGER
                     VSSSAVRAALAAGDLAAAERLLGRPYAMSGRIAHGEKLGRELGFPTANIALPQRAPLA
                     GIFVVECLIEGEAPWRPGVASVGRRPTVNPTAAPLLEVHLLDYAADLYRRHLQVRFLT
                     KLRDEQKYDDVPALRAAIAGDAARARSYFAMKTNA"
     gene            22178..24967
                     /gene="ileS"
                     /locus_tag="AC16O9_04045"
     CDS             22178..24967
                     /gene="ileS"
                     /locus_tag="AC16O9_04045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR6515822.1"
                     /translation="MPDYKKTLNLPDTPFPMRGDLAKREPQRVAEWRDKGVYRRLREV
                     AKGRPKFVLHDGPPYANGDIHIGTAVNKILKDIVVKSKSLAGFDAPYVPGWDCHGMPI
                     EVQIEKQHGKNLPAEETLRLCRAFAAEQIERQKADFRRLGVLGDWDDPYKTMAPRNEA
                     DEIRVLGKLLEKGYVYRGLKPVNWCFDCGSALAEAEVEYEDRKDPAIDVAFAFAEPAK
                     VAQAFGLAKLPDKPGYAVIWTTTPWTIPGNQALNVHPEFTYHLVDTEKGLLILAADLQ
                     AACLARYRLQGTTLGACKGKALERLRFRHPFYDRDAPVYLGEYVTLDTGTGVVHSAPA
                     YGVEDFDSCRRYGMPDEDILTPVMADGRFAASLPLFGGQSIWDANGAILKTLAERGAL
                     LAKENYVHSYMHCWRHKTPVILRATTQWFAGMEAVPGFRGAKPAETLRVTALRGVEAT
                     QFFPAWGQARLHGMIANRPDWTLSRQRQWGTPMPFFLHKETGQPHPRTLELFEEVAKR
                     VEKGGIEAWHTLKAEELLGAEAAQYDKGRDTLDVWFDSGSTHQTVLRGSHADLGFPAE
                     MYLEGSDQHRGWFHSSLLVSCMLNGVPPYKSLLTHGFVVDGEGRKMSKSKGNTVAPQE
                     VSNTLGAEILRLWVASTDYSGELSISNEILKRVVESYRRIRNTLRFLLANTSDFDAAK
                     DMLPVAQMVEIDRYALAMTARLQQDAAGDYARYQFHLVAQKLQGFCAEDLGAFYLDIL
                     KDRLYTCAAHSAARRSAQTALWHVTNALVRLMAPILSFTAEELWQVFTGKADDSVFLQ
                     TLYELPSPADAGALLKKWDRLRELRAPVQKRIEELRAAGKVGSSLQAEADLHVNGADH
                     ELLESLGEDLRFVLLTSAARLHRGDGEMRVDVAPSANAKCDRCWHYRPDVNGEGLCGR
                     CEVNLRGNGESRRHA"
     gene            24936..25424
                     /gene="lspA"
                     /locus_tag="AC16O9_04050"
     CDS             24936..25424
                     /gene="lspA"
                     /locus_tag="AC16O9_04050"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR6515823.1"
                     /translation="MATVSRAGTPRAWPWFLAAAGIVLADRVTKELMLAWLRPGESVE
                     VTGFFNLVLVFNRGAAFSFLADAPGWQTPLFAAVAIVAAAVVSWMLWRHPARTLLCSG
                     LALILGGALGNLWDRLLWGAVADFLDFHAFGWHWPAFNVADSAITVGAAILIAEGFMH
                     KD"
     gene            25426..26361
                     /gene="ispH"
                     /locus_tag="AC16O9_04055"
                     /gene_synonym="lytB"
     CDS             25426..26361
                     /gene="ispH"
                     /locus_tag="AC16O9_04055"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR6515824.1"
                     /translation="MAAVEVLLANPRGFCAGVERAIDIVERALAQIGAPIYVRHEIVH
                     NKTVVEDLRAKGAVFVEELDEVPAGATVVFSAHGVSKAVRADAERRGLKVFDATCPLV
                     TKVHVEVAKMLREGYDIVMIGHRGHPEAEGTVGQASRGMHLVETLADVARVEVSEGAK
                     LAYVTQTTLSVDDARAIVAALKARYPQIRGPKKDDICYATQNRQDAVKLMTPQCDVVI
                     VVGSPNSSNSNRLREVAENMGASAYMVDTAADLRAEWLAGCRRVGVTAGASAPEVLVD
                     EVVARLKALGAASVRQLDGITEHVAFTLPRELARR"
     gene            26348..28540
                     /locus_tag="AC16O9_04060"
     CDS             26348..28540
                     /locus_tag="AC16O9_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6515825.1"
                     /translation="MRGASKLARAAELYRAGRRRESEALLREVLTADPESSDALFYLG
                     CIAADAGRLGRALQLLNRAVARRPMEAAFHAALGNALARAGRADDALASFQAARALQP
                     GLAELLLNAGHALRALARHEEALASYDQLLARQPGHAAARLGRALALAALGRRDEALA
                     ELVQARSSAPAAFDYELEEAALRLALGEAGEAARRYARLLERPGADARLRLNVAAALI
                     QARDLKAALAQLRELERTGHRDAESVVALADLYSRCGEHGAALRLLEPLLAADDLPEW
                     RERVARELLELERHADAAEHLRKLIAVRPADPGTHANLGLALLGLDHVDEASAELREA
                     LRLAPRHWQALHGLGLALLKRGEIGEAIDAFEQARAIKADEPDLLSNLGYAYALARQF
                     ERGEPLLREAVALAPGHRRARRNLGLYHLVRGRFEQGWQHGEGPWDEHRLRTSGQLFA
                     QPWWQGEPLAGRSLLVWAEQGLGDQIMFCNPLPDLVAQGVRVTLQCEPRLARLFARSF
                     PGVDVVPRGPEGRARIERAAPELQVPMGQLPVYLRRSRAAFPRHRGYLRADETRVAYW
                     RARLESIGPAPRIGVSWVGGTVKTGKSRRSMTLEDWLPVLRLPGARFVSLQYTDCRAE
                     LAWLREAHGLEIVHWQEAIDDYDETAALVTALDAVASVCTAVVHLAGALDRPVRVAVP
                     WWPAWCFMLEGEEMPWYPSVRLIRQQEPNDWNAPMARIAEEIAGMARA"
     gene            complement(28537..28908)
                     /locus_tag="AC16O9_04065"
     CDS             complement(28537..28908)
                     /locus_tag="AC16O9_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6515826.1"
                     /translation="MRRGVLAWVGAAGILCLAPTAHGDAAEPKYWPAEKLEARPQIKT
                     HVMPEYPKDLPAGVRGRVVLELFVAPDGTLDRVRVASAEPRGRFEQAALKAFSAAGFT
                     PGIRKGKAVPSLVRIEVTFGN"
     gene            complement(28905..33896)
                     /locus_tag="AC16O9_04070"
     CDS             complement(28905..33896)
                     /locus_tag="AC16O9_04070"
                     /inference="COORDINATES: protein motif:HMM:NF017388.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HIR6515827.1"
                     /translation="MKTRIAFPYSQLTLALAGVFGLAATAGAANTDIANEPLSQAAAG
                     VKPNVMFILDDSGSMDWDYSPDYIDDVEPSGVSPSTTAACFDGGDTGPTFTNFSEDFD
                     DAAGSITGRADHCIAGDPPFMSPDFNKQYYNPAIWYRPGVNSDGSVKTGMSATNTSNW
                     TAVPTDPYGQQQDDQRGISKTTENLVTNYPDRAWCRNTTDAATDTTNCRVNSAYTYPN
                     WEFNRGFNNTSGYVSNYTHVKFRYGAPYYYRMQTAQWCSTPTLNAGTCVSGSSVNPSA
                     HIYIAPEFCTDPELVNCAAGAALTPAHIYSGARWCSDKGTLLNCQRKKVGNFRWPKHL
                     GTTAVSTVNIPPVSATGTLTVTQTELNEKVNTLTVDGISIIAGPVTAGGSSTSAFATL
                     IRDAINAFVSTPEYTATVSGSTVTIAAAATGAGENGKTIVVTTSQTGTVGALGQITVN
                     NGGANAQYIEGIFVVHPTSGSINIMSTSCTGSFGSGVSLIGGGTYGNHIRSNNGTNSG
                     AERTATAAAIAACINQGSSGFTATQSGQSVDVRAPTSLGSLMNGRSITMDQTSGSYFT
                     SSFGGGVSTGAVVSTSGSMAGGADTTTGTLPLRIGVGQFSRVNIVSSNNSYPKGSGRF
                     DCAGATCTYAEEMTNFANWYSYYRTRMQMMKSAAGRAFIPIDDTYRVGFITINPQSPV
                     SSSRYLRIQNYDTVHKGNWYTKFYAQGPSGGTPLREALSRVGRLYAGRFDGINTGIPA
                     ADDPMEVSCQPNFAILSTDGYWTTVGSSGNKIDGTAMTQQDNANVGPYSLRADGVYDG
                     SPAGATVGLADTALYYYQTDLRPTGSLNAASVDVSANNVPTTQQDFAAHQHMTTFTLG
                     LGLDGTLTYRNDYQTAGSGDFLAIKQGTLDWPNPVANTPQALDDLWHAAVNGRGVFFS
                     ASNPQELANSLTDTLDALKKRVGAGAAAATSNLQPVAGDNFAFTAQYQTQDWIGDLKA
                     RTIDLNTGAISQVELWSAQALLNGRAHTSRTLFTFDATDPYVSTSVTDVGNQLKHFCA
                     SAAPVAPWCIDGLGLTLAEQAYFNPNQLPQWTSISGNPLQVPNATVENLVNFLRGHQA
                     FEDQGNASGTDLFRERTALLGDIINAQPAYVRSSPFNYNDTGYKDFKTCSEGTTTVTC
                     PAAQFPSPTIPRRPTVFIAANDGMLHAIETDVNNDPYYQTGGIPTGSTADDTFSGGNN
                     AGNGVERWAYIPGIVLPNLYKLASMPYGHRYFVDGSPTVGDICISTPCAGLDDWRTML
                     VGGLNSGGRGFYALDVTNPLKPRAMWEFKVRKPSVTACAATPAAAVGAKDDCDLGLSF
                     GNPVITKMKATGKWVVMVTSGLNNTGFEPSTANRQGDGMGYLYILDALTGAIMHKIPT
                     NVGSPGTLANSYTDAVPSGLAKVNNWVTSSLTDNTTLAVYGGDMLGNLWRFDLDNTSA
                     TYLTAVKVAELKDAFANPQPITTKPELGLINSRRVIFVGTGRFLGNSDKADTRKQSIY
                     AIADDLAGNVTVTDRTPLVQQTITAIGSTSRTVSSVNVVDWTNAAVRGWFIDLPDKGE
                     RVSVDPQLQLGTLVVGSNVPSTDTCVAGGTAWINTFDYRTGGYVAGATANAVSQKISG
                     SVAVGINVIQLPGGKVVTVVTTADNQQLPVETPIAPASFQGRRVGWRELNADQ"
     gene            complement(33920..34501)
                     /locus_tag="AC16O9_04075"
     CDS             complement(33920..34501)
                     /locus_tag="AC16O9_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR6515828.1"
                     /translation="MNTRPLPRARQRGAVLFIALIVLVAMSLAGIALIRGVDTANLIA
                     GNLAFKQNATHGGDWGVELARAWLQAQAAATLYNDVPGRYSAAMQSGVDFTATDPSAP
                     DFDWAANSFDAGADPAGNQVRYVIHRMCDLAGNPGSVNCVRTSLGGTTGGTQGGATYG
                     GAALPSTSQIYYRITARVTGPRNTVSYVQVMVN"
     gene            complement(34498..35646)
                     /locus_tag="AC16O9_04080"
     CDS             complement(34498..35646)
                     /locus_tag="AC16O9_04080"
                     /inference="COORDINATES: protein motif:HMM:NF027400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR6515829.1"
                     /translation="MTMPLKSNFRITAPARMAGMSLMEILIAMAIGLIGIVVITQTYL
                     VNENYKRSTTSAGGAQTNGALALFSIEREARMAGWGISWSTALGCTNIQWFYNGDYSS
                     PPGGGLPVLTLAPIVITDGGAGPDTITIMYGSGTERVIPATLSKSMPSPSAELEVDNP
                     QGFSDTPGDMVLLVQGGTCSMTQITQVQPAAGHLQHGPGVTAPYNPPGGGSLLPAFTA
                     GAQVFNLGRPTVNVYSISNNALQLATLFSATSSTVVPAYTPTPFTIVDNIVDLQAEYG
                     KDNGVNNGTVGAVAYTANDGIVDSYDNVTPANGVEWQQVLSVRIGVLARSEHFVKPEN
                     VGDPCSATTAAPTWRGGAFAVPGGIPSCYGYRVFETVVPLRNMIWRQG"
     gene            complement(35643..36053)
                     /gene="pilV"
                     /locus_tag="AC16O9_04085"
     CDS             complement(35643..36053)
                     /gene="pilV"
                     /locus_tag="AC16O9_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HIR6515830.1"
                     /translation="MRKTQSGVALLEALVGILIFSIGILALMGLQAQSIRNTVEAKYR
                     NEAAYLANQIIGQMWVDRANLAAYDTGGGANPNMTAWRAQVANTLPRVVAGGTNSPTI
                     VVAGNQVTVTIFWQLPGSDSVLRQFSVVAQINDS"
     gene            complement(36050..36661)
                     /locus_tag="AC16O9_04090"
     CDS             complement(36050..36661)
                     /locus_tag="AC16O9_04090"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR6515831.1"
                     /translation="MLITRTTQSGVSLIELLIGLAIMGLLIMIGLPNMATWLQNSKIR
                     TAGETMLAGLTLARTEAVRRNQIVRFQMVNNLTSACVISTSGTDWVVSLDDVTGACDA
                     APSETAAPRVIQRRSGSEGTTSVVLAAATAGVVHFNGLGRVTSPGGAANMTQVAISNP
                     VGGTCEHVDGGAMRCLRINISVGGEARMCDPAVTDASDPRICP"
     gene            complement(36655..37104)
                     /locus_tag="AC16O9_04095"
     CDS             complement(36655..37104)
                     /locus_tag="AC16O9_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6515832.1"
                     /translation="MRSARGFTLIELMITVAIVAILAAVALPAYTDYVTRSKFTEAHS
                     HLADLRVKMEQRFQDARTYVGGPCAPTGPGGAQVKYFTFTCPAAATANTFVLQAAGMV
                     GTELEGIAFTVDESNQRATVVTPATSMAAKGYTSNAACWITKKPSQC"
     gene            37287..37362
                     /locus_tag="AC16O9_04100"
     tRNA            37287..37362
                     /locus_tag="AC16O9_04100"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37320..37322,aa:Thr,seq:cgt)
     gene            37375..37755
                     /locus_tag="AC16O9_04105"
     CDS             37375..37755
                     /locus_tag="AC16O9_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6515833.1"
                     /translation="MAARILVVDDDESIRDLLRLHLTSAGYEVEVAADAIAAGYKVLK
                     SPPDLIITDVRMPHMDGFEFVAALRADTTVPRIPVIFLTSVEDGDARGRELGAVGYIT
                     KPVRSDRLLSLVAQHVPGGAIPIG"
     gene            complement(37768..38448)
                     /locus_tag="AC16O9_04110"
     CDS             complement(37768..38448)
                     /locus_tag="AC16O9_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6515834.1"
                     /translation="MRLLFLLLLAANLGFFAWMRFAPPDPAVDRQPLAQQLEPHKLRI
                     VSERALGKAPPPAPAKPKPAPPAPEPARLACLEWGSFGPAEATRAAQRLEPLGLGARL
                     AQYRGEETASWWVHMPPQGSRANALRKAGELKKLGVEDYFILQDAGPMRWALSLGVFS
                     TEEAARSHLQALREKGVRTAIIDRRETRVPKVWFQVREVEPPLQEKLQAMAREFEGAT
                     LHDCAPRS"
     gene            complement(38445..39167)
                     /locus_tag="AC16O9_04115"
     CDS             complement(38445..39167)
                     /locus_tag="AC16O9_04115"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6515835.1"
                     /translation="MILAIDAGNSRVKWGWHDGRAWSSLATVSLIEFAAANHDINPFA
                     ATHANPERIVISNVAGEGAHQLLVNWTSIFDAEPFWLKGEAERCGVTSRYERPGQLGA
                     DRWAALVAARALHTGPCLVVQAGTATTADLLSADGEFLGGLILPGVDLMRFVLHEHTG
                     RLPLQEGNFVRTPRNTVDAIETGCRHAQAGAVERMYRVAGAGAQCLVSGGAGPSLIEQ
                     LEIPCRYVENLVLEGLARIAWS"
     gene            complement(39164..39919)
                     /locus_tag="AC16O9_04120"
     CDS             complement(39164..39919)
                     /locus_tag="AC16O9_04120"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR6515836.1"
                     /translation="MDRLNAGAISVPGVDVRVVARCASTNTVLLADDLRRPALLAADE
                     QTAGRGRRGRRWHSPPGCGALFSLGLALARPARELSGLSIVAGVAAVRALRALGAAET
                     ALKWPNDLLLGGAKLGGILVQTRAQGAGSAAVIGIGVNWRRAPGLAARLRRGIAALQD
                     GLQPLPPRNAVIGALAREVLAALRAFDAAGFEPFRGDWEAVHAYAGRALRVRLADGRV
                     FAGVAAGLAVDGALQLRTRTGLRTVRNGRVAPA"
     gene            complement(39937..>40648)
                     /locus_tag="AC16O9_04125"
     CDS             complement(39937..>40648)
                     /locus_tag="AC16O9_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6515837.1"
                     /translation="DDVTLEIRRGQTLGIVGESGSGKSTVARCIVRLIDPTRGEIRIH
                     GEDIATRPMSALREMRRKVQIVFQDPYRSLNPRRTVGEAIIEGPLNYGLPRAEALERA
                     RKLMELVRMDPAVLERYPHQFSGGQRQRICIARALAMEPELLIADEAVSALDVSVQAQ
                     VLALLEEIRERFNLAMLFITHDLRVAAQICDHVAVMSQGRVVEYGPAAEVFGAPRHAY
                     TRALFDAAPGRHFAFGAA"
BASE COUNT         5965 a        13846 c        14473 g         6364 t
ORIGIN      
        1 taccgcggcg acggccccgg cgcggccgag ccgcagccca tggagacctg cggcggtgac
       61 ccctgccatc caacgggagg cgacggcttg caccggccgc cgcgagcccg catcattccg
      121 ggtccaagac cgcacggccc ggcgatgacc tccgatccac gcttcccgct gctcagcgcg
      181 atcgacgacc cgcacgacct gcgcgacctc ccggagagca agctcccggc ggtggcggcg
      241 gagctgcgcg agttcctcgt cgacagcgtc ggcaagaccg gcggccacct ggcctcgagc
      301 ctcggcaccg tcgagctgac ggtcgcgctg cactacgtct accagacccc ggacgaccgc
      361 ctgctgtggg acgtcggcca ccaggcctac ggccacaagg tgctgaccgg gcggcgcaag
      421 cttatgcaca cgctgcgcca gggcggcggc ctgtcgggct tcctgaagcg ctccgagagc
      481 ccctacgacg ccttcggcgc cggccactcg agcacctcga tcagcgccgc gctgggcatg
      541 gccatcgcct ccaagcgcca gggcaagccg caccgggtcg cggcgatcat cggcgacggc
      601 gcgctgaccg ccggcatggc gttcgaggcg ctgaaccacg gcggcgacct cgacgccgac
      661 ctgctggtgg tgctgaacga caacgacatg tcgatctcgc cgaacgttgg cgggctctcc
      721 aactacctcg ccaagctgct ctccgggccg gtctacaccg gcatgcgcga gggcggcaag
      781 cgggtgctga gccacctgcc gccgttccag gagctggcgc ggcgcgccga ggagcacctg
      841 aagggcatgg tgatgcccgg cacgctgttc gaggagctcg gcttcaacta catcggcccg
      901 atcgacggcc acgacctcga cacgctgatc gcgaccctca ccaacatcaa gaagctcaag
      961 ggcccgcagt tcctgcacat cgtcacgagg aagggccagg gctacaagct cgccgaggac
     1021 gacccgatcg cctaccacgg gccgagcaag ttcgatcccg ccgaaggcat caagaaggcg
     1081 gccgcgccgg ccaagcccac ctacagccag gtgttcggcg actggctgtg cgacatggcg
     1141 gcggccgatg cgcgcctgat ggcgatcacg ccggcgatgc gcgagggctc gggcatggtg
     1201 cagtactcgg agaagttccc cgagcgctac ttcgacgtcg gcatcgccga gcagcattcg
     1261 gtgagcttcg ccgccggctt ggcctgcgag gggctgcgcc cggtggtggc gatctactcc
     1321 acctttctgc agcgcggcta cgaccagctg attcacgacg tcgccatcca gaacctgccg
     1381 gtgaccttcg cgctcgatcg cggcgggctg gtgggcggcg acggtgccac gcacaacggc
     1441 gcgttcgatt tcgcctacct gcgcacggtg cctaacctta cggtcatggc gccagcggac
     1501 gcccaagagt gccggcgcat gctcagcacg gcgtttcacc tcggtgcgcc ggcggcggtg
     1561 cgctacccgc gtggcgcagc gctcggcgag gcgggcagcg cactcgacac cctgccggtg
     1621 ggcaaggggc agctgcgccg tagcggccag cgcgtcgcga tcctcgcgtt tggctcgatg
     1681 ctgaagcccg cgctcgaggc ggccgaggcg ctcgacgcca ccgtggccaa catgcgcttc
     1741 gtcaagccct tggacacgga gctgctgcgc gagctcgcgc aggggcacga gctgctcgtc
     1801 acggtcgagg agcaccagat catgggcggg gcgggcagcg cggtctgcga ggcgctcgcc
     1861 ggcctgcgca tcgagaagcc cgtgctgctg ctgggcctgc ccgaccgctt catcgaccac
     1921 ggcgatccgg ccaagctcct gtcgtcggtc gggctggatg cgcccggcat tgaaacggcg
     1981 atccgcgccc tgatatccga gtaaattact cgggagttat aatcgccgca tgaaagccaa
     2041 ggacctgcac aatatcccgg acgtccagag cacgcccgat tcgcgcaagc tcgcgatcga
     2101 caaggtgggc atcaaggcga tccgccaccc gatccagatc atgcagcgcg cgggcggcgt
     2161 gcagcacacg atcgccaact tcaacatgta cgtcggcctg ccgcaccact tcaagggcac
     2221 gcacatgtcg cgcttcgtcg agatcctgag cgcgcacgag cgcgagatca cggtggattc
     2281 gttccaggcg atggtgaagg agatggtggc caagctcgag gccgaggaag gcgtggtcga
     2341 gatgaccttt ccctacttca tcgagaaggc cgccccggtg tcgcgcgtga agagcctgat
     2401 ggactacgag gtcagcttcg tctcgcagat cctcgacggc cggcagggct tcgccatgaa
     2461 ggtgctggtg ccggtgacga gcctgtgccc gtgctcgaag aagatctcgg cctacggcgc
     2521 gcacaaccag cgctcgcacg tcacggtgac ggcgcacacc ggcgagttcg tgtggatcga
     2581 ggagatcgtc gacctggtgg aaaagcaggc ctcgagcgag ctctacggcc tgctgaagcg
     2641 cccggacgag aagcacgtta ccgagcgcgc ctacgacaac cccaagttcg tcgaggacat
     2701 ggtgcgcgac gtggccgcgc tcctcaatct cgacgagcgc atcaccaggt tcgtggtcga
     2761 gtcggagaat ttcgagtcga tccacaacca ctccgcctac gccctcatcg agcgcgacaa
     2821 gacggctggc tagctgcgtg ctggccgggc tcggcatcag cgccgtcggg gcccaggact
     2881 tcgacgcacg ccgcatgcgc ctgatcgacg aaattgacgc catgtacgcg gcgacccgcg
     2941 ccgagaccgg cctcgccacg atgtcgcccg cggtgcgcgc ggcgatggcg cgcgtcgagc
     3001 gccaccgctt cgtgcccgca gccgagcgcg atcgcgccta cggcaactac ccgctgccga
     3061 tcggtgccgg gcagaccatc tcgcagcctt acatcgtggc gctgtcgacc gacttggtcg
     3121 cgccgcgccc cggtcacgcg gtgctcgaga tcggcaccgg ctcgggctac caggcggcgg
     3181 tgctcgccga gatcgtggcg caggtgtaca gcatcgagct gatcccggac ctggggcgcg
     3241 cggcggcggc gcggctcgcc gcggcgggct acgggaacgt cgaggtgcgc atcggcgatg
     3301 gctacgccgg ctggcccgag aaggcgccgt tcgacggcat cgtggtgacg gcggcggcgc
     3361 cgcgcgtgcc gcaggcgctt gtcgatcagc tcaagccggg cgcccgcctg gtgatcccgg
     3421 tcggggcgac acacgcggtg caggagctgc tcgtcattac caagcgcgcc gacggcggca
     3481 ccgacgagcg ccgcgtgctg ccggtgcgtt tcgtcccgct cgttcccggc cgacgctagt
     3541 agcgccgcgt gagggtcatc agctggccct cgttcttcat ctcgacgcgg ctgaggaagc
     3601 tcatgccgag cagcgcgatg tccagcccct gctcgatgac cacgccgtcc accgcgttga
     3661 tctcgatcgc gcccaggcgc acgctgtcga ggcgcacgcg gtacaccggc accacgccgg
     3721 ccgcggtctt ggtgagcgcc acctcgccct tgcggtaatc gagcccgagg cgcagggcat
     3781 cggcggccgg caacgccacc atggtggcgc cggtgtcgat gacgaagcgc acggcgcggc
     3841 cgttgatcgc gccctcggtg atgaaatgcc cgcgcgtgtc ggccgcaagc gtcacgctct
     3901 ggcgcgcatc gcccgccccc gtgctgtagt gctggccgcg cgccagcgtg cgctgcgtgc
     3961 ccgccacctc gatcaccgcg ctatcgcgct ccacggagac cacggtgacg ccagccacct
     4021 cgcgcccgat gcgcaccgtg cgcggcccgc cgccgtcgat caccaggatc gccgccttgt
     4081 cgccgaaagt gccgaccagg cgcacgtcca gggcgtgcgc cgccgccggc gcgagcgcca
     4141 ggacggcgag cagcgccgca ctacaatgcc gccacatgaa gccgattgcc atattccgcc
     4201 atgcccccag tgaaggtccg ggctattttg ccagctatct cgagcgcaag gcgatcgcct
     4261 ggcggctcgt gccgctagac gctggccacg cggtaccgcg cagcacgcgc ggcttctccg
     4321 gccttgtctt catgggtggg ccgatgagcg cgaacgacga gctgccgtgg attattccgg
     4381 cgctctcgct gatcgccgac gccgtgcgca atgacgtgcc ggtgctcggg cactgcctcg
     4441 gcggccagct catggcgcgc gccttcggcg gggaggtgcg cgccgcgccg gtgacggaga
     4501 tcggctgggg cgaggtcgcg gtggccgaca atgccgtggc gcgcgagtgg atgggcgcga
     4561 tcgaggcctt cgagtcgttc cactggcacg gcgagacgtt ctccatcccg cccgggggca
     4621 cgcgggtgct ggagaacgcg cactgcgcca accaggcgtt cgcgctcggg cgccacttcg
     4681 gcatgcagtg ccacgtggag atgacgccgg agatgatcgc cgagtgggtg cgtggcggcg
     4741 cagcggaaat cgcgcagtcg cgggattcgc ccggcgtgca ggcgcccgac gagatcgaac
     4801 gcaacgtcga gcggcgcacg gcggcgctgc acgcagtggc cgatcgtatc tacgaccggt
     4861 ggtgcgaggg actcagtcgc ggaagttgat gtattgcaac ggctggtcgg tcaccgactg
     4921 ccgcaccagc tggatcgccg cctgcaggtc gtccttcttc ccgcccgaga cccgcaccag
     4981 gtcgccctgg atcgaagcct gcaccttgag cttcgcgtcc ttgatcgcct tttggatctg
     5041 cttcgccttt tcctgcgcga tgccgacctt caccgtgacc acgcgcttcg ccttgtcgcc
     5101 ggcggccttt tccaccgcgc cgagctcgag gcagcgcacg tcgatcgagc gcttggccat
     5161 cctctggcgc agcacgtcga taacctggcc gagcttgtac tcgtcgtcgg cgaacaccgt
     5221 gagcgccatc tcggcctgct cgatgcgcgc gtcggagccc ttgaagtcga accggttctg
     5281 gatttccttg ttggcctgct cgaccgcgtt cttcacttcc tgggcgttga cctgcgagac
     5341 gatgtcgaaa gagggcatgg acagggattc tacgcgcgcg cgccgcgcag gtagacgggc
     5401 cggcccgaaa gccggtcggg ggcggcagcg agcggccggc cgaaggcgtc ctcggcgcgc
     5461 tcgaaggctt cgtcggcggg aaacgccagc gcgggcccgt gggcataagt cagcgccacg
     5521 atttcggcgt cgccgcggcg aaatgcgaag cggtagcggc cgtggcgctc gccgcggcgc
     5581 ccgggcaggc gagcgccaat gaaggtgctg gcgccgagca ggcgcaggaa atgctgcagc
     5641 atggcgtacg cgggtcgcgg ccgcagcgcc gccgggtcgg cgtcgtccac caggccgaag
     5701 ccgcgcgccg cgaggcgcca ccagtagacg cgctcgacca tccccgagcc gagcgcgagg
     5761 cacaggtagc gcagcaggta atccgcgtac tcgtcctcgc tcgtgccggg atcgctcgcg
     5821 tggcgccacg gcgggtagta gggcgcgtgg atgggcgcgt gcgcgccggt gccgcgcagc
     5881 ggccagttca cttccgacac cacgaagcga tcggcgcact gcggcgcggc acgcgcgagg
     5941 gcgcggccca gggtgaactt ctccgccgcc ccgaagccgt tctgcggatt ctcgggcgcg
     6001 ccgcgccggt ccacgtacag gtggtgcgag agcgcggcga gcggcacgcc ggctggccac
     6061 tcgcgcagca cgggaaaggc gatcgggtac tcgaagtcga tgagcgcggg acccatgaag
     6121 cgcagcgcgg gaaagcgcgc ctgcagctcg gcgagcggcg cgtacagcgc gcgcaggtcc
     6181 tcgaagcccc agatgcccca cttgacgcgg ttgacggcgt ggccgacttc gactgcttcg
     6241 accacgcctg ccacgtcctc cagcgcctcg aacgcgaaac gccgccagga ttcgggttcg
     6301 cacaccgcgc ggcggtcctg cacgagcgcc acgctcacgg cgtgcccggc gcgatgcagc
     6361 gcgtgcacca gcgccgcgcg ccagcgccgc gtctcgcggg cgtcgtggtg atagaagcgc
     6421 accaacgcgg gcagcgggcc gagcccggca agcagcgcaa gctcgccctc cagcgtctgc
     6481 ggcgtgggct cgagcgccat gccgacgcgg ccgccgagcc cgacgggctg cgtgtaggca
     6541 agcggcagca gaccgcggta ctcgcgccag gcgcgcggcg cgtcgcgcag cgcgtcgacg
     6601 agcacgcgcg tgtagcgcga gcgcgggaaa tggcgcacgc ggtcgcggcg cagcagcgcg
     6661 ctgatcggct gcgcggtgtc ttcgtcccag atccaaacgt cgcgcggtgc ggggtagtcg
     6721 cgggcagcgg gatcggggcg ccggccgcgc agcgcgctgc gcacccggcg caggcgcact
     6781 ttccatgcat agcgttcgcg atagcgccgc tgccagtcga gcagctcgcg cggcgggacg
     6841 ccgcgccagt acatctcgat cagcatctgc agcaggtccg agggcagttc gaggcgcgag
     6901 aagtcgcgcc cccgctcgcg cagcgtgagc tcgccgcgca ggcgtccgcg attgaggtcc
     6961 acgaccatga agtcgcgcca gccggcttcg cccgcgggcc gcagcagcac gttctgcgcg
     7021 ccgaggtcgt tgtgcacgaa tcccgccgcg tgcatgccgc gcatgccgtc ggcaacgcgc
     7081 tcgagcagcg cgacgaagtg cgccgccagc ggcgggcgcg cgtcgtacag cgcgagcagc
     7141 gcttcgcgca gggtctgggc gccgtcctgg tactcggcaa ggtagtagct ctcgcgcagc
     7201 cgcgcgccgt cccagcgctc gaggtagccg atcggcggcg gcgtgccggc gccttgcgcc
     7261 gcgaggtgcg tcgccgctcg ccaggtgcgc gcggccttgg agccgcggcc ccggtcgcgc
     7321 agatcgcgaa accacggctg cacgccgaac gccttcacca tcacgccgcg cgcaccgtct
     7381 tgcagcggca gttcgacgac gatgttgcgg ttgcgtccct ccgcgaccag gcgggcgccg
     7441 ggcgcttcga gcagcaccgg caggcgggcc agctgcgcca gcagctccgg cgcgcggtaa
     7501 ggcgccgcga tctcgccggt gtaggcgccg gcgacggtgg gcgccgctgc gctagcgcgc
     7561 attcaggcgc tggagctcgc tcgtccagcg ctgcgtgatg tcgagcagcc aggccgtgtc
     7621 ggccgcgtca cgcaagccgg tgtacgcgga cacggagagg accttgccgc gcagcagcag
     7681 ctgcgtggtg gtgtacagca gatactccgg cgtgtcgccg tagcgcggcg gcaggcgcgt
     7741 gcccatgagc ctggagacgg cgagcggctc gctgcgcagc acgcgcagca cattggcgcg
     7801 gccgagcgcc tgggcgctca ggaacgcccg gatatcctgg ggctgcgccg cctcgcccag
     7861 gctgcgtgcc gactccactg cgtagtcgcg gaactggctc aggccgacgc tgtcgcgctc
     7921 cagtgcgcgc ggtgtcgcga gcagcaggta gcggtgcagc gcgggcgcat cgccgcggct
     7981 gaacaggcgc aggtcggcgt cggtcagcgc gaacagcagg atccggttgg aggccgaggt
     8041 caggctctcg gcgagctcct gcagccgcgg cgagccgaga tcgagcgtgt cggcgaagcc
     8101 cggcggcgta tcgagcacca cgcgcatgtc gccgacgcgc gccgcgaacg gctcggcggc
     8161 gcagtcgagc cagagcacgg ctagcagcga gatggcgagg gcgcgcacgg ccgccattgt
     8221 agccaccatc acgtatcatc cctagttttc ggagcgcagg aacgtggcca ccatcctcag
     8281 cacgctgccc acgggcgaaa gagtcggcat cgccttctcg ggaggcctcg acacgagcgc
     8341 cgccgtgcac tggatgcgcg cgaaaggcgc ggtgccgtac gcctacaccg cgaacctcgg
     8401 ccagcccgac gagtccgact acggcgcgat cccgaagaag gcgctcgcct gcggcgccga
     8461 ggtggcgcga ctggtcgaat gccgcccgca gctcgtcgcc gagggcatcg ccgcgatcca
     8521 gtgcggcgcc ttccacatct ccacgggcgg cgccacctac ttcaacacca cgccgctcgg
     8581 ccgcgcggta accgggacga tgctcgtcgt cgccatgcgc gaggacgcag ttggcatctg
     8641 gagcgacggc agcacctaca agggcaacga catcgagcgc ttccatcgct acggcctgct
     8701 cgcaaacccg tcgttgcgca tctacaagcc ctggctcgac cagcgcttca tcgacgagct
     8761 gggcggacgc aaggagatgt ccgcctacat ggcgcaggcc ggtttcgact ccaaggtgag
     8821 cgccgataag gcctattcca ccgactcgaa cctcctcggc gccacgcatg aggcgaagga
     8881 cctcgagttc ctcgaccggg ggctcgggat cgtcgagccg atcatgggcg tggccttctg
     8941 gcgcgagggc gtgaaggtga agcccgagcc ggtctcggtg cgcttcgagg ccggggtccc
     9001 ggtggcgctg aacggccagc agttcaccga cgcggtcgcg ctcatggccg aggccaacgc
     9061 catcggcgga cggcacggcc tgggcatgag cgaccagatc gagaaccgca tcatcgaggc
     9121 gaagagccgc ggcatctacg aggcgccggg catggcgctg ctgcacatcg cctacgagcg
     9181 cctcgtcacc gggatccaca acgaggacac catcgagcag taccggacga acggccggcg
     9241 cctgggccgg ctcctgtacc aggggcgctg gttcgacccg cagaccatga tgctgcgcga
     9301 gaccgcgcag cgctgggtgg cgaaggccgt aacgggcgag gtggcgctgg aacttcgccg
     9361 cggcaacgat tggtcggtgc tcgacacgag gagcccgaac ctcacctaca agcccgagcg
     9421 cctgacgatg gagaagggcg aggcgttctt cacgccggcc gatcgcatcg gacagctgac
     9481 gatgcgcaac ctcgatatcg cggatacgcg ggacaagctc cgcgtctacg gcgaggcggg
     9541 gctgctcggc gagtcgaaag acggcggcgt gccgctgctc gggcccgaaa aaccgggcaa
     9601 gcgctagcgg cgggctcgtc cgcgcccggg cgcgccccgc aacgccgccg cggcaaaccg
     9661 cgccgcctgc gcgttggagg cggcgacaac cacgcccgcg tcctccagga tcctcgcctg
     9721 cctagaggac acctgcggat cgccgtcggt gccggtcacc gacgcaatga ccggcttgct
     9781 cttgttcttc agcagcgctg ccgccagccc ggcggggtcc ggatgggccc catggcccag
     9841 gacgaagtcc agaagcacca gcccgacctc cttgtcgcgc agcgcccgcg ccaggtacgc
     9901 gttgcgcagc tccggctcga tcatcgggtg cggccggccg cgcgtgtaat cgtcggcgcc
     9961 gagatcgatc agcaggtgcc cggcgcgcgc cggcgcggcg cccgggaccg gcacattgga
    10021 ggagacgtcg aggccatggt ccataaggac gatctgcgcc tcggcgcaca gcgtgccgcc
    10081 gcagaatagg ccgcggatgt tgcctttcgc cgccagtcgc ggcggggcgc ctcgggcagg
    10141 cttgccgctc gcctgttcca gggtacgcgc cagactgacg ccacgaggca cggccagctc
    10201 gtcggcaccc agcaggcaga gcgtgaccgg ttttctgctg cgcccggcgc gcgccacgat
    10261 ccgcttcacc accgcgggcg cgggcggctt ggaaatgacg atgatgcgcc gggtcgccgg
    10321 gtcggcctcg agcgcatcga gcgccgcgag cgtcatgcga gcgccgaccg cctccgacag
    10381 gtcgcgcccg ccaacgccaa ttccgtgcga gacgcccttg ccctgccgcg cgagcagcgt
    10441 cgagacttcc tgcaggccgg tgcccgaagc ggacacgatg cccacgtcgc cgcgggatac
    10501 cgcgttggcg aagccgatcg gcgcgccgcc gaggagcgcc gtgccgcagt ccggtcccat
    10561 catcagaagc ccgcgctcca gcgccaggtc cttcagcgcg acttcgtccg caaggggaac
    10621 gttgtccgag aacatcatca cgtgcagccc gtgctcgagc gcgcggcgcg cttcggcggc
    10681 ggcgtactcg ccgggcaccg agatcagggc gaggttggcg cccgccaggc cggcgatcgc
    10741 ggaggcaagg gtgggaaagc tttcaccggc ggcgcgcgtc tgcgttcctt tttgcagcag
    10801 cttttcggcg agcgccagcg ccgcctgcgc cttgggtccc cggacggcga tgatgaggtc
    10861 atcgggcccc gccgcatcgc ccgccttcgt cagcagtccc gaagccttca gcagcgcctt
    10921 gttcgacggc gtgccgatca tgagggacgc cgtcacgccg cccttgttca gctcgcgcgt
    10981 gatgcgcatc agcaccacgg aatcggcgta gaagccccgg cgcagccggt tgacgacgct
    11041 catggccggc tgccgagtgc gcgcacgagc gctgccagcg cctggtcgaa gcacgccagg
    11101 ggtgcgcgcg cgacgccggc gccgacctgg ccgacgccgg gccgccggtg cgcaatcccg
    11161 gtgttgatgg cgggctggat gccggtgtcg acgaccttgc gcgcatcgat gccggcggga
    11221 acgccccggg cgtcgagcgc cgggatcgtc cacctcggat tcgcgcccac ggtcagctcg
    11281 cccatcgcac gcgtgaacgc cagcgcatcg ctggcgcggc ccgcgccgac gaaacccatc
    11341 acggccggcg aggcggccat ggcgaaagcg cccaggccca cggtctccat gatcgccgag
    11401 tcgcccatat cgggattggc gtcgcgctcg ccatagccgg cgaagtaaag gccctgcggc
    11461 atctccaccg gcgccgtgaa ccacgcatcg ccggtggccg agagccggat gccgaaatcg
    11521 gtgccgttgc ggcacatggc ggtgacgagg ctgcacccct cgatgccgcg caccgggtcc
    11581 atgacgctct ttgccattgc catgccgacg ttgaggaaga attgctcgtt gccggcgatg
    11641 aacgcgaggc tctgcgccag aggctcggcg ccacccgccg cgcgcgccag atgcggcgcg
    11701 agcccgcgca gcaccagcgc cgagcaggcg acgttgcgct ggtgcatctc gtcgcccatc
    11761 gcgaggccgc gcgccacgag cggcgcgagg tcgagtccgc ccgccgcgcg cagtgcggcg
    11821 cccagccgcg gcccgaactc atcgcgcagc cacgcgatgc gcgcaagtac ctcggcgtca
    11881 tttgcgccga agcgcatcac cttgccgagg ccctcgttga tcatgcagta gctgcggttc
    11941 ccgaaagcgc ggttctccac caccatcacg ggcatggagc gcgtggtgat gccggtcatc
    12001 gggccgaccg cgccgagcgc atggttcggc cggaagtcga tcgcgccgct ggcggcgagt
    12061 cgagtagccg ccgcgagatc cggcgcccag ccttcgaaca ccagagcgcc ggcgacggcg
    12121 ccacgcatcg gcccgcacat gcggggccag tcgatcggcg gtcccgcgtg cagcacgaca
    12181 tgccggtcga gaccgggcat cgcctcgccc gccggcagaa cgtccaccag cgccggctcg
    12241 gccgaaagca tgcgccgcag cccttccgcg ttcgcctgcg cgatgcgcgc ttcatgccgc
    12301 gcgagagcgc cgagcagcgc cgcggcgtgc tcgcgaccgg ccgccggtgg ccgccactcg
    12361 acgtgcgcca ccgcaacgtc gcgacccgcc agctccgcgg cgaatgcgcg cagaccgacg
    12421 ttgaccacgc gcggcggccg tgcgagcagc gacgcgatcg gcgaggacgg acgggacatg
    12481 gggagcggta ttatcgcagc atgcccgagg tctccactgc gcagcgtctc gttgtcgcca
    12541 tcggcggcaa cgccacgcat ccggaaaaca tccgtggcac gaccgcagag caggaggaga
    12601 ttgccgcgcg cgcggcgcgc gcgctgcggc cgctcgtcga ggcggtaccc gagatcgtca
    12661 tcacgcatgg caacggcccg gtcgtcggca agatcatgct gcgccagtac tacgcgcgcg
    12721 agcacgtgcc gccgatgcgg ctggacgtgt gcgtggcgca cagccagggc ggcatcgggc
    12781 acctgctgat gcaggggctc gaggacgagc tggcgcgcgc cgacatcgag cggcgggtcg
    12841 cctgcatcgt gacgcgcgtg gaagtgaacg agggcgacga ggcgttcacg cactcgacca
    12901 agccggtggg gccgttcttc agcgccgagg aggccgccga gctcaagcgc acgctcggct
    12961 ggaacatggt cgaagacgcc ggccggggct ggcgctatct cgtgccttcg ccgcgtccgc
    13021 gccgcatcct cgacctggat ctcatcgccg gcctcgcccg gcagggcgtc gtggtgatcg
    13081 ccgcgggggg cggcggcatc ccgatggtgc gccgccccga cggctcctac gccggcgtgc
    13141 ccgcagtcgt ggacaaggac ctcgcctcgg cgctgctcgc cgccgagctc cattacgaca
    13201 cgctgctcat cctcaccgcg gttccgcgcg tgctggtgca tttccgcacg gcgcgcgagc
    13261 acgcgatcga gtcggtgagc gccgccgagc tgcaggcgct gcaccgcgaa ggccatttcg
    13321 ccgagggcag catgggcccc aaggtcaggg cggcgctgga tttcctcgcc gccggcggac
    13381 ggcgcgcgat catcgcgcac ctcgaccagg cggccgacgc gctcgccggc gcggcgggca
    13441 cgcatgtcct gccgggttga ggcgatcgag gcgggctctg cggcgtggcg cacggcaggc
    13501 gacgcgcggg tcttcgcgtc tttcgagcgc tgctgctacg tggaaacggc ggcgggcatc
    13561 atgtgcctgg gcggccccgg gctgggacgc ggccccctga atgttctggt gggagcgtat
    13621 tcgcactact cggtcggcga tcggatcgag atcgatcttt cctcggcggc gcgctggcgc
    13681 cccgagctgc gcttggcgac gcaccgtagc gttccggccc gcatccgtga accctggcga
    13741 gatcgcggcg aggcgcaggc gttcctgcgc tggattgccg gtgcgggggg ccggccgaat
    13801 gatgcgttga tcgggcttgg ggcgggcctg acgcccgcgg gcgacgactt tgtcggcggc
    13861 gcgatgatcg cgctgcgcgc tgccggcgag ctcgccctcg ccgagcgtat cgccgcgtgg
    13921 gcgctgtcgc tcgccgagac ccgcaccggc cggatcagcc gggcgcacct cgcgtgggcg
    13981 gcgcagggcg agggccacga ggcgctgcac gccttgctcg cctcaaacga aggcagcgcg
    14041 cgcttcgagc agagcctcgc ccggctcacg cggatcggcc atacgtccgg gctcgacgcg
    14101 gctgccggcg cgctgctcgc gctcgccgcg cgccgcggtg gctcaggcct tggcccgggc
    14161 cttgaccttg ccgagcagca ggaactcgat tagcgccttc tgcgtgtgca ggcggttctc
    14221 ggcctcgtcc cagacgacgc tcgccggccc gtcgatcacc tcggcggcga cctcttcgcc
    14281 gcggtgcgcg ggcaggcaat gcatgaagag cgcgttcttc ttcgccgcct tcatcatctc
    14341 ggcgtccacc tgccagtcct cgaagtcgcg cttgcgcgcc tcgttctcgg cttcgaagcc
    14401 catgctggtc cagacgtccg tggtgacgag gtcggcgttc ttcgccgcct gcatggggtc
    14461 gcggaactcc tcgtaatggc cggtgccgta caggcccgcg cgctcgggct cgacctcgta
    14521 gcccggcggc gtcgagacgt gcacgttgaa atcgagcacc tcggcggcct gcagccaggt
    14581 gttgcagacg ttgttcgagt cgccgatcca ggccacggtc ttgccgcgga tcgcgccgcg
    14641 ctgctcgacg aaggtataga tgtcggcgag cacctggcac gggtggtact cgttggtgag
    14701 gccgttgatc accggcacgc gcgagtgcgc ggcaaagcgc tcgatgatgt cctgctcgaa
    14761 ggtgcggatc atgacgatgt cgcacatgcg cgagatcacc tgcgcggcgt cctcgaccgg
    14821 ctcgccgcgg ccgagctgcg agtcgcgcgt ggacaggaag atcgcggcgc cgccgagctg
    14881 gtgcatgcct gcctcgaacg acaggcgcgt tctcgtggac tgcttctcga agatcatcac
    14941 cagggtgcgg tcttccagcg gccagtagcg ccggtagcgc ttgaactgcg ccttgatcca
    15001 cttggtgcgc gcgaacaggt gctcgcactc ggcgcgggcg aggtccttga attgcaggaa
    15061 atggcgcagc ttcgccatgc gtggacgccg gccggcctca gcgcgccgcg gcggcgcgcg
    15121 gcgcggcgga ctgcgcgagg aactcgcgga tgatcggcac caggcgctcg accacctggc
    15181 ggccctcgtc ggcgttcatg acgagcggcg gcaggaggcg gatcacggta tcggcggtga
    15241 cgttggtcac cagcccgcgc tcgagcgccg tcttcacgat gtcgccgcac ggccggtcga
    15301 gctcgacgcc gatcatcatg cccatgccgc gtacctcggc gacgccggcg acgccggcga
    15361 gcgccgcccc gaggccctcg cggatggcgt cgccgaccct ggccgcgttg gcgagcagat
    15421 cttcttcctt catggtgtcg agcgtcgtca ccacgccgca catcgcgagc gggttgccgc
    15481 cgaaggtcga gccgtggttg cccggcttga acacgccttt ggcgcgcccg ccgaccacgc
    15541 aggcgccgac cggcacgccc gaggcgaggc ccttggcgag cggcaccacg tcgggctgga
    15601 tgcccgcgtg ctggtacacg aaccacttgc cggtgcgacc gaggccgcat tgcacctcgt
    15661 ccgacatgaa tagccagtcg ttgcgctcgc agatctcctt cagcccgcgc aggtactcca
    15721 ggcgcgagat gttgatgccg ccttcgccct ggatcggctc gatgaacacc gccaccacgt
    15781 tcgagttgcg ctcggcgacc tggcgcacgg cgtcgaggtc gttcagcggc acgcgcacga
    15841 acccctgcac cagcggctcg aagccggcct gcaccttgcg gctgccggtc gccgagagcg
    15901 tggccaggga ccgtccgtgg aacgcctttt ccatgacgat gatcgcgggc tccgcgatgc
    15961 cgcggtcgtg gccgtacttg cgcgcgacct tgagcgccgc ttcgttcgcc tccaggccgg
    16021 agttgcagaa gaacacctcg tccaggccgg tgatctcgca gatgcggtcc gcggcctgct
    16081 cctgcagcgg catctgaaac aggttagagg tgtggatcac gcgcccgatc tgctcggtga
    16141 gcgcgcgcac caggcgcggg tgggcgtagc ccagcgtatt cacggcgatg ccggcgaggg
    16201 cgtccaggta cttcttgtcc gcctcgtcgt acagccacac gccctggccg cgcacgaacg
    16261 cgaccggcaa gcgggcgtaa gtgttcatga cgtggctcat ggcgcctcca cacggttggc
    16321 caatgaaaaa cggcggccca agccgccgtc acagggatgt taatgggcta gccgatgcgg
    16381 tgcaagccgc gcgcttcggc gggtctgagc acgcgcacgc agggctggta gccctcgtac
    16441 accacgccgt ggatctccat gcgcttctgc gaccgcagca ggctggccag gtactcggca
    16501 atgtcgcgcg tgatgacctg cccgggcacc agcaccggaa tgccgggcgg gtagggcacc
    16561 acctggtcgg cgcagacgcg ccgggcgagc gacttgttga cccgctcctt gtcatcgaac
    16621 accggcacca gctcgccgcc gcagtagtag gcgtcgcgcg gcaggtagcg gagcttcgtg
    16681 aaggcgggga tctcgggcgt gcgcacgagg cgccgcggag cgcgcttctc gcgcgcgatg
    16741 cgcatgaagg cgtcgtacag gcgcgagacc ttggagcgcg tggtgccgat ggtgagcagc
    16801 agcgtcagcg tgttgaaggt gctcttttcg gtctggatgt tgaagcgctc gaacagaagc
    16861 ttctgcaggt cgtcgaccgt gtagccgcag ccggagatgt cgacagtgac cttggtcggg
    16921 tcgaggcgga tgccgtcgtg ccgcagctcc tcgggcagca ggtcctcgag ctcgaggctc
    16981 cggaacacct tggtggagtt gatctgctcg cggagctcct tggcgagcgc cagcgtgcgt
    17041 gaaagcagct tgtagccctc catcaccgct tgcttgcgcg ccacgtcgag gctcgcgatc
    17101 agcccgtact gcggactggt cgaggtgtgc atgttgaagt tctcgcggaa caggtgctcg
    17161 cggaagtccg ggtcgttgac gtggatcatc gaggcctgcg agaaggccga caagaccttg
    17221 tgcgtggact gcgtggcgta gtctgcgccc gcctcgagcg cggtggggcg gaactccgga
    17281 tggaagcgcg cgaagccgta ccaggcctcg tcgatgatca ctttgatctt cttcgcgtgc
    17341 gccgcctcga cgatcggctt caggtcgtag cgcaggccgt cgtaggtgca cgaagttagg
    17401 atcagcgcct cggcgtccgg gtgccggcgg atcgcctcga gcaccgtctt tttcggcacg
    17461 gggccgtaca gcccgtacct gcggttgagc gccgagtcca ggtacacggg gtgcgcgccc
    17521 gagagcacca cgccgtggtg cacgctcttg tggcagttgc gatcgagcag cagtttctcg
    17581 cccggtgcga gcagcgtctg gaagatcacc ttgttggcgg tcgaggtgcc gttggtggcg
    17641 aagaacgtgc gccgcgcgcc gaacgcctgc gccgccatga gctgcgcctc ggcgatcacg
    17701 cccgtcgggt ccatgaggga gtcgagcatc ggcaccgaga ccgagagatc ggcgtcgaac
    17761 acgtgctcgc ccatgaactc gtagaagtcg ttcacccagg ggctgccgcg cagggattcg
    17821 cctgaggagt gccccggcgt gtgccactga tccttcgcca tccacacata gttcttcagc
    17881 tggtcggtga agggtgtcct cgcgcgctcc tggatctgcg cgttgaggat gcggtagatg
    17941 ccccggtagt cgcgctcctc gcggtagaag tagccgtcta ccgattcggt gaacagcgcg
    18001 tctaccacgt cgtcttcctt ttcctgcgcg atcaggatgt agaccgatag ctcggggcga
    18061 aagcgcgtga tgcgctgcac cagctcgagc gcggagacct gcaggcggcg cgagccgttg
    18121 tgcttggcca gcgtatagag cgtgtcgtcg accagcacgg cctggatgtc gccgtcggac
    18181 tggatggcgg caagtgcttc cttggcggtg gtgacgccgg cgaaggtgat gccgagggga
    18241 ttgtcgagcg agcgcgccgc ggcgttcagg cccttgacga actcgcgcaa caccagaagc
    18301 tcgtcgttga tgacgaggat gcggctgacg ggcttcgcca tgaggggcgc aagtataatc
    18361 cggcatgccc gaggactcgg cggccgagcc cgcccggcgc cagcgcgccg agcataccgg
    18421 atgcagcctt actgcatcgc cttgatcgcc tgcgcgaggc ggctcatgtg gcgcgaggcg
    18481 gcgttgcggt gcaggatgcc cttggcggcc gtgcggtcga tgacgctgcg cgacttctcc
    18541 agcgctgcgg cggccgcagc cttgttgccg ccggcgatgg ccttcttcac gttcttgatc
    18601 gcggtgcgca cttcggtgcg cagggtgaag ttgtgggcgc ggcgcacgac agcctggcgg
    18661 gccgacttgc gcgaagattt gatgttggcc atgaccttga tccggtggcg aaaaaggggc
    18721 gcattctagc atgaacctgc tgcgcgtcct ggccaccgtc agcagcatga cgttcctgtc
    18781 gcgggtgctg ggtttcgcgc gcgacttctt catcgcgcgg ttcttcggcg cgggcctcgc
    18841 caccgacgcg ttcttcgtcg ctttccgcat cccgaacctg ctgcggcgca tgtttgccga
    18901 gggcgcgttc tcgcaagcgt tcgtgcccat cctttccgaa tacaagaacc ggcagagcgc
    18961 cgaggagacg cgctcgctgg tggacgtcat cggcaccctt ctcttcgtca tgctggtcgt
    19021 ggcgacgctc atcggcatgc tggccgcgcc gctcatcggc tacctcttcg tgccgcgctg
    19081 gttccatgcg gagccggaca aattcgcgct gacggtgcag ctgctgcgca tcacctttcc
    19141 ctacatcctg ttcatctcgc tggtggcgct cgccgccggc gtgctcaaca cctggagccg
    19201 gttcgcggtg ccggcgttca cgccggtgct gctcaacgtg tcgttcatcg tcgccgcggc
    19261 gttcttcgcc gagcgcttcg atcccccggt gctggcgctt gcctgggccg tgttcatcgg
    19321 cggcgcgctg cagctcgcgc tgcagctgcc gttcctcgcg cgcatcggca tgctgccgcg
    19381 ctggcggctc gatctgggcc accccggcgc ccggcgcgtc ctgacgctga tggtgcccgc
    19441 ggcgttcggc gtctcggtga gccaggtgtc gctgctcatc aacaccatct tcgcttcgtt
    19501 tctcgttacc ggcagcgtgt cgtggctgta ttacgccgac cgcctgatgg agtttcccgc
    19561 gggaatgctc ggcgtcgcgg tgggcaccat cctgctgcca agcctctcgc gccactacgc
    19621 gagcggcgcg gccgcggagt acgcgaagct gctcgactgg ggactgcgcg tgacgctgct
    19681 gctcgcgctg cccgcggcgg cagccctggc ggtgctcgcg gtgccgctga tcacgacgct
    19741 gttccactac gggcgcttcg gcgccgacga cgtctggatg acgcgccagg cgctcgtggc
    19801 ctacagcctg ggtctggtcg gcatgatcct ggtgaagatc ctggcgccgg ggttctacgc
    19861 tcgccagaac gtggtgacgc cggtgaagat cggcctggtg acgctcgcgg ctacgcagct
    19921 catgaatctc gcgttcatcg tcccgctcaa gcacgccggt ctcgcgcttg ccatcggcct
    19981 gggggcgtgc ctgaacgcgg cgctgctcta ccgcaatctg cggcgccatg gcatctacgc
    20041 accccagccg ggctggggcg ccttcgccgg ccgcatcgcc tttgcggtga cgtcgatgtc
    20101 ggcggtgctc tactttgcga tggggccgag cacgtggtgg ctgcaagcgc cctggcagca
    20161 caaggcggcg ggaatgtgcg gcctggttgc gctcggcgtt gccgcctatg gcctcatcct
    20221 gctggcgctc ggcttccact ggcgcgactt cgtgcgccgc gaggcaccct gatgaacgtg
    20281 ccggacccct ttgccgagct catggcgcgg gacgaggccg aaatcgacct cgcgcgcgcc
    20341 tgcctgctta tcgccaccga cgcttacccg gggctcgacg tcgagggcta cctgggcgag
    20401 atcgagcggt tggcggcgcg cctgcgcggc cggctggctc cgggcggcgg cgccgaggag
    20461 cgcgtgctgg cgctgaacca gtttctgttc gacgacctgg gctacagcgg caacgccgac
    20521 aattactacg acccgcgcaa cagcttcctg aacgaggtgc tcgaccggcg caccggcatc
    20581 ccgattacgc tctcggtgct gtacctggag atcgggcggc gcatcgggct ggaactggag
    20641 ggcgtatcgt tccccgggca cttcctcgtg cgcctgcgct tgcgcggcgc catgctggtg
    20701 ctcgatccct tctcgggtgg cgaggcgctt tcggagtccg atctgcgcga gcgcctgcag
    20761 cgcgtgatcc ccgagggcgc ggccggtggc gtgccggtcg gcgcgctccc gctcgatccg
    20821 tttctcgagc ccgccggtaa gcgccagatc ctcgcccggt tgctgcgcaa cctgaaaggc
    20881 atctaccgcg aagcggacaa gccgcagcgc ctgctcgacg tcctgaaccg catgctggtg
    20941 gtggcgccgg aagcgcatgg cgagttgcgc gagcgggggc tgctctacca gaaactggag
    21001 tgctaccgcg ccgcgctcaa ggacctgcag gattactcgg cgctcgcgcc cgaagcaccc
    21061 gacatcgacg acgtgcgtgc caagctggtc gagctctcgg cgcagtgcgc ccggctcaac
    21121 tagccgagaa atcggggcgg cttgtcgcgc gggtcccggc gctgggtgca cgggtacccg
    21181 tctgcggctc gagcgcccgc atcgtcgcat ccgcgaaagc cgctataatt cagcatttta
    21241 cacgcgctga tgcgggttac gcacggtttg acggcgccgg caggcaagcg gctggcgctc
    21301 accattggca atttcgacgg cgtgcaccgc ggtcatcagg cgatgctcgc gcgcacggtg
    21361 gcgcgcgcgc gcgagctgcg gctcgatgcc tgcgcgctca ccttcgagcc ccatccgcgc
    21421 gagttcttca ccccggcgac cgcgccggcg cgtctgacgc gcctgcgcga caagctcgag
    21481 ctgatggcgg cggcgggcct ggatcgggtg cacgtggcgc gctttgatgc gcggctcgcg
    21541 tcgctccccg ccgagcggtt cgtcgacacc tacgtggcgc acgggctgcg cacggcgtgg
    21601 ctgctcgtcg ggcgcgactt ccgcttcggc gccaggcgcg gcggcgactt cgccctgctt
    21661 ggcgagctcg ccgggcgtca cggcttcgag ctggaggcga tgccggaggt tgcgctgcac
    21721 ggcgagcgcg tctcgagctc cgcggtgcgc gcggcgctgg cggccggcga cctcgcggcg
    21781 gccgagcgcc tgctcggccg accctacgcc atgagcgggc gcatcgcgca cggcgagaag
    21841 ctcgggcgcg agctgggctt cccgaccgcc aacatcgcgc tgccgcagcg cgcgccgctc
    21901 gcgggcatct tcgtggtcga atgcctgatc gagggcgaag cgccgtggcg gccgggcgtg
    21961 gcgagcgtgg gccggcgtcc cacggtgaat ccgaccgcgg cgccgctgct ggaggtgcac
    22021 ctgctcgact acgccgccga cctgtaccgg cgccacctgc aggtgcggtt cctcaccaag
    22081 ctgcgcgacg agcagaagta cgacgacgtc ccggcgctgc gcgcggcgat cgcgggcgat
    22141 gccgcgcgcg cccgatccta ctttgcgatg aagacgaatg cctgactaca agaaaacgct
    22201 gaatctgccc gacaccccgt tcccgatgcg cggcgacctc gccaagcgcg agccgcagcg
    22261 agtcgccgag tggcgcgaca agggcgtcta ccggcgcctg cgcgaggtgg ccaaggggcg
    22321 cccgaagttc gtgctgcacg acggcccgcc gtacgccaac ggcgacatcc atatcggcac
    22381 cgccgtgaac aagatcctga aggacatcgt ggtgaagtcg aagtcgctgg cgggcttcga
    22441 tgcgccgtac gtgcccgggt gggactgcca cggcatgccg atcgaggtgc aaatcgagaa
    22501 gcagcacggc aagaacctgc cggccgagga aaccttgcgc ctgtgccgcg cgttcgccgc
    22561 cgagcagatc gagcgccaga aggcggattt ccggcgcctg ggagtgctcg gcgattggga
    22621 tgacccgtac aagacgatgg ctcctcgcaa cgaagccgac gagatccgcg tgctcggcaa
    22681 gctgctcgag aagggctacg tgtaccgggg cctcaagccc gtgaactggt gcttcgactg
    22741 cggctcggcg ctcgccgaag ccgaggtgga gtacgaggac cgcaaggacc cggcgatcga
    22801 cgtcgccttt gcgttcgcgg agcccgcgaa ggtggcgcag gccttcggcc tcgccaagct
    22861 tcccgacaag cccggctacg cggtgatctg gaccaccacg ccctggacga ttcccgggaa
    22921 ccaggcgctc aacgtgcatc ctgagttcac ctatcacctc gtggacacgg aaaagggcct
    22981 gctgattctc gcggcggacc tgcaggcggc atgcctcgcg cgctacaggc tgcagggcac
    23041 gacgctgggc gcgtgcaagg ggaaggcgct cgagcgcctg cgcttcaggc atccgttcta
    23101 cgaccgcgat gcccccgtgt acctgggcga gtacgtgacg ctcgacacgg gcaccggcgt
    23161 cgtgcactcg gcgccggcct acggcgtcga ggacttcgac tcgtgccgcc gctacggcat
    23221 gccggacgag gacattctca cgcccgtcat ggccgacggc aggttcgcag cctcgttgcc
    23281 gctcttcggc gggcagtcga tctgggacgc caacggcgcg atcctgaaga cactcgccga
    23341 gcgcggcgcg ctcctcgcca aggaaaacta cgtccacagc tacatgcatt gctggcggca
    23401 caagaccccg gtcatcctgc gcgcgacgac ccagtggttc gcagggatgg aggcggtccc
    23461 cggtttcagg ggcgcaaaac ccgcggagac actgcgcgtc accgccctgc gcggcgtcga
    23521 agcgacgcag ttctttcctg cctggggcca ggcgcggctg cacggcatga tcgccaaccg
    23581 gcccgactgg acgctctcgc gccagcgcca gtggggcacg ccgatgccgt tcttcctgca
    23641 caaggagacg ggccagccgc acccgcgcac gctcgagctg ttcgaggagg tcgcgaagcg
    23701 cgtggagaaa ggcggcatcg aggcctggca cacgctgaaa gcggaagagc tgctcggcgc
    23761 cgaggccgcg cagtacgaca agggccgcga tacgctcgac gtctggttcg attccggctc
    23821 gacgcaccag accgtgctgc gcggctcgca tgccgatctg ggctttccgg cggagatgta
    23881 cctggagggc tccgaccagc accgcggttg gttccactcc tcgctgctcg tgtcgtgcat
    23941 gctgaacggc gtgccgccgt acaagtcgct gcttacgcac ggcttcgtcg tcgacggcga
    24001 gggccgcaag atgtcgaagt ccaagggcaa caccgtggcg ccgcaggagg tctccaacac
    24061 gctgggcgcc gagatcctgc gcctgtgggt ggcctcgacc gactactcgg gcgagctgtc
    24121 gatctccaac gagattctga agcgcgtggt cgagagctac cggcgcatcc gcaacacgct
    24181 gcgctttctg ctcgccaata cctcggattt cgacgccgcg aaggacatgc tgcccgtcgc
    24241 gcagatggtg gagatcgacc gctacgcgct cgccatgacg gcgcggctgc agcaggacgc
    24301 ggccggggat tacgcgcgct accagttcca tctggtggcg cagaagctgc agggcttctg
    24361 cgccgaggac ctgggcgcgt tctacctcga cattctcaag gatcgcctgt acacctgcgc
    24421 cgcgcattcg gcggcgcgcc gctcggcgca gacggcgttg tggcacgtga cgaatgcgct
    24481 cgtccggctg atggcgccga tcctctcctt cacggccgaa gagctgtggc aggtcttcac
    24541 cggcaaggcc gacgacagcg tgttcttgca gaccctgtac gagctgccgt cgcccgcgga
    24601 cgccggcgcg ctgctgaaga agtgggaccg cctgcgcgag ctgcgcgcgc cggtccaaaa
    24661 gcgcatcgaa gagctgagag ccgcgggcaa ggtcggctcc tcgctgcaag cggaggccga
    24721 cctgcacgtg aacggcgccg accacgagct gctggagagc ctcggcgagg acctgcgctt
    24781 cgtgttgctt acctcggcgg cgcggctgca ccgcggcgac ggcgagatgc gcgtcgacgt
    24841 cgcgccgagc gccaacgcca agtgcgaccg ctgctggcat taccggccgg acgtcaacgg
    24901 cgagggcctt tgcgggcgct gcgaggtgaa cctgcgtggc aacggtgagt cgcgccggca
    24961 cgcctagggc ctggccctgg ttcctggcgg cggcgggcat cgtgcttgcc gatcgcgtga
    25021 ccaaggagct gatgctcgcc tggctgcgcc cgggagaaag cgtcgaggtg acggggttct
    25081 tcaacctggt gctggtgttc aaccgcggcg ccgcattcag cttccttgcc gacgcgccgg
    25141 gctggcagac gccgttgttc gccgcggtgg cgatcgtcgc cgccgcggtg gtgagctgga
    25201 tgctgtggcg ccatccggcg cgcacgctgc tctgcagcgg cctcgcgctg atcctgggcg
    25261 gtgcgctcgg caacctgtgg gatcggctgc tgtggggcgc ggtcgccgac tttctcgact
    25321 ttcatgcgtt cggctggcac tggccggcgt tcaacgtcgc cgactcggca atcacggtcg
    25381 gcgcggcgat cctgatcgcc gagggcttca tgcacaagga ctagaatggc ggccgtggaa
    25441 gtcctgctcg ccaatccgcg tggattctgc gccggggtgg aacgcgccat cgatatcgtg
    25501 gagcgcgcgc tcgcgcagat cggcgcgcca atctacgtgc gccacgagat cgtgcacaac
    25561 aagaccgtgg tggaagacct gcgcgccaag ggcgcggtat tcgtcgagga gctcgacgag
    25621 gtgcccgccg gcgccacagt cgtattcagc gcgcacggcg tgtcgaaggc ggtgcgcgcc
    25681 gacgccgagc gccgcggcct caaggtcttc gacgccacct gcccgctggt caccaaggtg
    25741 cacgtcgaag tggccaagat gctgcgcgag ggctacgaca tcgtcatgat cggtcaccgc
    25801 ggccatccgg aggccgaggg caccgtgggg caggcatcgc gcggcatgca tctggtggaa
    25861 acgctcgcgg acgtggcgcg cgtcgaggtg tccgaaggcg cgaagctcgc ctatgtgacg
    25921 cagacgacgc tgtcggtgga cgacgcgcgc gcgatcgtcg cggcgctcaa ggcgcgctat
    25981 ccgcagattc gcggaccgaa gaaggacgac atctgctacg ccacgcagaa ccggcaggac
    26041 gcggtgaagc tcatgacgcc gcagtgcgac gtggtgatcg tggtcggttc gcccaacagc
    26101 tcgaattcca accgcctgcg cgaagtggcg gagaacatgg gcgcaagcgc ctacatggtg
    26161 gatacggccg cggacctgcg ggccgagtgg ctcgccggct gccgccgtgt cggcgtcacc
    26221 gcgggcgcat cggcgccgga agtgctggtg gacgaggtcg tggcgcgcct caaggcgctc
    26281 ggtgccgcga gcgtgcggca gctcgacggc atcaccgagc acgtggcgtt cacgctgccg
    26341 cgcgagcttg cgcggcgcta gcaagctcgc gcgggcggcc gagctgtacc gcgccggacg
    26401 gcggcgcgaa tccgaggcgc tgctgcggga ggtgctcact gccgatccgg agtcgagcga
    26461 cgccttgttc tatcttggct gcatcgccgc cgacgccggc cggctcggcc gtgcgctgca
    26521 gcttctcaat cgcgccgtgg cgcgccgacc catggaggcg gcgttccacg cggcactggg
    26581 caacgcgctt gcgcgcgccg gacgcgcgga cgacgcgctc gcaagcttcc aggcggcgcg
    26641 cgcgctgcag ccgggtctgg ccgagctgct gctcaatgcc ggccacgcgc tgcgcgccct
    26701 cgcacgccat gaagaagcgc tcgcctccta cgatcagctg ctcgcgcgcc agcccgggca
    26761 cgcggcggcc cggctcgggc gcgcgctcgc gctcgcggca cttgggcgcc gggacgaggc
    26821 gctcgccgag ctcgttcagg cacgctcgag cgcgcccgcc gctttcgact acgagctcga
    26881 ggaagccgcg ttgcgactgg ccttgggcga ggcgggggaa gcggcgcggc gttacgcgag
    26941 gcttctcgag cgcccgggtg cggatgcacg gctgcgcctc aacgttgccg cggccctcat
    27001 acaagccagg gacctcaagg ctgcgctcgc gcagctgcgc gagctggagc gcactggcca
    27061 tcgcgatgcc gagagcgttg tggcgcttgc cgatctctac agccgttgcg gcgagcacgg
    27121 cgccgcactg cggcttctcg agccgctgct cgcggccgac gatctgccgg agtggcgcga
    27181 gcgcgtcgcg cgcgagctgc tcgagctcga gcggcacgcc gacgccgccg agcacctgcg
    27241 caagctgatt gccgtgcgtc cggccgatcc cggaacgcac gccaacctcg gcctggcgct
    27301 gctcgggctc gatcatgtcg acgaggcgtc ggccgagctg cgcgaggcgc tgcgccttgc
    27361 gccgcggcac tggcaggcgc tgcacggtct tggcctcgcg ctgctcaagc gcggcgagat
    27421 cggcgaggcg atcgatgcgt tcgaacaggc gcgcgcgatc aaggccgatg agccggactt
    27481 gctgagcaat ctcggctatg cctacgcgct cgcgcgccag ttcgagcgcg gcgagccgct
    27541 gttgcgcgaa gcggtggcgc ttgcgccagg gcaccggcgc gcgcgccgaa atctcgggct
    27601 gtaccacctc gtgcggggac gtttcgagca gggctggcag cacggcgagg ggccctggga
    27661 cgagcatcgc ctgcgcacga gcgggcagct ctttgcgcag ccgtggtggc agggcgagcc
    27721 cctggcaggg cgttcgcttc tggtgtgggc ggagcagggc ctcggcgacc agatcatgtt
    27781 ctgcaatccg ctgccggacc tcgtggcgca gggcgtgcgc gtgacgctgc aatgcgagcc
    27841 gcgcctggcg cgcctctttg cacgctcctt tcccggtgtc gacgtcgtgc cgcgcgggcc
    27901 cgaaggccgc gcgcgtatcg agcgtgcggc gcccgagctg caggtgccga tgggccagct
    27961 gccggtgtac ctgcgacgct cgcgcgcggc gttcccgcgc caccgcggct acctgcgcgc
    28021 ggacgagaca cgcgtcgcct actggcgcgc gcgtctcgag tcaatcgggc cggcgccgag
    28081 gatcggcgtg tcctgggtcg gcggcacggt gaagaccggg aaatcgcgcc gctcgatgac
    28141 gctggaggac tggctgccgg tgctgcggct gccgggggcg cgctttgtca gcctgcagta
    28201 cacggactgc cgcgccgagc tcgcgtggct gcgcgaggcg cacggtctcg agatcgtgca
    28261 ctggcaggaa gcgatcgacg attacgacga gacggcggcg ctcgtcacgg ccctcgacgc
    28321 ggtggcgagc gtgtgcacgg cggtggtgca cctcgccggc gcgctcgacc ggccggtgcg
    28381 ggtcgccgtg ccgtggtggc cggcgtggtg cttcatgctg gagggcgagg agatgccctg
    28441 gtatccgtcg gtgcggctca tccgccagca ggagcccaac gactggaacg cgcccatggc
    28501 gcgcatcgcg gaggagatcg cggggatggc gcgcgcctag ttgccgaacg tcacctcgat
    28561 gcgcaccagg ctgggcacgg ccttgccctt gcggatgccg ggcgtaaagc ctgccgccga
    28621 aaaagccttg agcgcggcct gctcgaagcg cccgcgcggc tcggcactgg cgacgcgtac
    28681 gcggtccaat gttccgtccg gggcgacgaa cagctccagc accacgcggc cgcgcacgcc
    28741 cgccgggaga tccttcgggt actccggcat gacgtgcgtc ttgatctgcg gccgcgcctc
    28801 gagcttttcg gccggccagt atttcggctc ggcagcgtcg ccgtgcgcgg tgggcgccag
    28861 gcagaggata ccggcggcgc ccacccacgc gaggacgccg cgtctcactg gtcggcgttc
    28921 agctcgcgcc agcccacgcg cctgccctgg aagctggcgg gcgcaatcgg cgtctcgacc
    28981 ggcaactgct gattgtccgc ggtggtcacc accgtcacca ccttgccgcc cgggagctgg
    29041 atcacgttga tacccaccgc cacggagccc gagatcttct ggctgaccgc gttcgccgtg
    29101 gcgcccgcga cgtagccgcc cgtgcggtag tcgaaggtgt tgatccaggc cgtaccgccc
    29161 gcgacgcagg tgtcggtcga aggcacgttg gagccgacca ccagggtacc gagctgcagc
    29221 tgcggatcga cgctgacgcg ctcgcccttg tcgggcaggt cgatgaacca gccgcgcacc
    29281 gcggcgttgg tccagtccac cacgttaacc gacgacaccg tgcgcgaggt ggagccgatc
    29341 gcggtgatcg tctgctgcac cagcggcgtg cggtcggtga ccgtgacgtt gccggccagg
    29401 tcgtcggcga tggcgtagat cgactgcttt ctcgtgtcgg ccttgtcgga gttgccgagg
    29461 aagcggccgg tgcccacgaa gatcacgcgc cggctgttga tcaggccgag ctcgggcttg
    29521 gtggtgatcg gctgcgggtt ggcgaacgca tccttcagct ccgccacctt taccgccgtg
    29581 aggtaggtcg cgctcgtgtt gtcgaggtcg aagcgccaca ggttgcccag catgtcgccg
    29641 ccatagaccg caagggtcgt gttgtcggtg agcgagctgg ttacccagtt gttgaccttg
    29701 gccaggccgc tcgggacggc gtccgtgtag ctgttggcga gggtgcccgg actgccgacg
    29761 ttggtcggga tcttgtgcat gatggccccg gtcagcgcgt cgaggatgta aaggtacccc
    29821 atgccgtcgc cctggcgatt ggcggtgctc ggctcgaagc cggtgttgtt caggcccgaa
    29881 gtcaccataa ccacccactt gccggtcgcc ttcatcttgg tgatcacagg gttgccgaag
    29941 gacaggccca ggtcgcagtc atccttggcg ccgaccgcgg ccgcgggggt ggccgcgcac
    30001 gcggtgacgc tcggcttgcg caccttgaac tcccacatgg cgcgcggctt gagcgggttg
    30061 gtgacgtcaa gggcgtagaa accgcgcccg ccggaattga gaccgcccac cagcatggtg
    30121 cgccagtcgt ccagccccgc gcacggcgtg ctgatgcaga tatcgcccac ggtcggcgag
    30181 ccgtccacga agtagcggtg gccgtagggc atcgacgcca gcttgtacag gttcggcagc
    30241 acgatgcccg ggatgtaggc ccagcgctcg acgccgttgc cggcgttgtt gccgccggag
    30301 aacgtgtcgt ccgccgtgga cccggtcggg atgccgccgg tctggtagta cggatcgttg
    30361 ttcacgtcgg tctcgatcgc gtgcagcatg ccgtcgttgg cggcgatgaa cacggtcggc
    30421 cggcgcggga tcgtcggcga cgggaactgt gcggccgggc aggtcaccgt ggtggtgcct
    30481 tccgagcacg tcttgaagtc cttgtagccc gtgtcgttgt agttgaaagg cgacgagcgc
    30541 acgtaggccg gctgcgcgtt gatgatgtcg ccgagcagcg cggtgcgctc gcggaacagg
    30601 tcggtgccgg aggcgttgcc ctggtcctcg aatgcctggt gcccgcgcag gaagttcacg
    30661 agattttcca cggtggcatt gggcacctgc agcggattgc cgctgatcga ggtccattgc
    30721 ggcagctgat tggggttgaa atacgcctgt tcggcgagcg tcaggcccag accgtcgatg
    30781 caccacggtg caaccggcgc cgcgctggcg cagaagtgct tcagctggtt gccgacatcg
    30841 gtgacgctgg tcgagacgta cgggtccgtg gcgtcgaacg tgaacagcgt gcggctggta
    30901 tgcgcgcggc cgttgagcag cgcctgcgcc gaccacagct cgacctggga gatcgcgccc
    30961 gtgttcaggt cgatggtgcg cgccttcagg tcgccgatcc agtcctgcgt ctggtactgc
    31021 gcggtgaagg cgaagttgtc gcccgccacc ggctgcaggt tcgaggtcgc cgccgccgcg
    31081 cccgcgccga cccgcttctt cagcgcgtcg agggtgtcgg tgaggctgtt ggccagctcc
    31141 tgcgggttgg atgcgctgaa gaacacgccg cggccgttga ccgccgcgtg ccacaggtca
    31201 tcaagcgcct gcggcgtgtt ggccaccggg ttcggccagt cgagcgtgcc ctgcttgatg
    31261 gcaaggaagt cgccgctgcc ggcggtctgg tagtcgttgc ggtaggtcaa cgtgccgtcc
    31321 aggcccaggc ccagcgtaaa ggtcgtcata tgctggtggg cggcgaagtc ctgctgcgtc
    31381 gtcggcacgt tgtttgccga cacgtcgacg ctcgcggcgt tgagcgagcc ggttggccgc
    31441 aggtcggtct ggtagtagta cagggccgta tcggcgaggc cgaccgtggc gccggccgga
    31501 ctgccgtcgt agacaccgtc ggcgcggagc gaatagggtc cgacgttggc gttgtcctgc
    31561 tgcgtcatgg cggtgccgtc gatcttgttg cccgacgagc ccaccgtggt ccagtaaccg
    31621 tccgtggaca ggatggcgaa gttcggctgg caggacactt ccatcggatc gtcggctgcc
    31681 gggatgccgg tgttgatgcc gtcgaacctg cccgcgtaca ggcggccgac gcgcgacagc
    31741 gcctcgcgca gcggcgtgcc gccgcttggg ccctgggcgt agaacttggt gtaccagttg
    31801 cccttgtgca cagtgtcgta gttctggatg cgcagatagc ggctcgagct caccggcgat
    31861 tgcgggttga tggtgatgaa gccgacgcgg taggtgtcat cgatggggat gaaagcgcgg
    31921 ccggcggccg acttcatcat ctgcatgcgc gtgcggtagt acgaatacca gtttgcgaag
    31981 ttggtcattt cctccgcgta ggtacaggtg gcgccggcgc agtcgaagcg ccccgagccc
    32041 ttcgggtacg aattgttgct ggagacgatg ttcacgcgcg agaactggcc gacgccaatc
    32101 cgcagcggca gcgtgcccgt cgtcgtgtcg gcgccgcccg ccatgctgcc ggaggtcgat
    32161 accaccgcgc ccgtggacac accgccaccg aagctggagg tgaagtagct gccgctagtc
    32221 tggtccatgg tgatgctgcg gccgttcatg agcgagccaa ggctcgtcgg cgcgcgcacg
    32281 tccacggact ggcccgactg ggtcgcggta aagccggacg agccctggtt gatacacgcg
    32341 gcaatcgccg cggcggtcgc cgtgcgttcg gcgccggaat tggtgccgtt gttcgaccgg
    32401 atatggttgc cataggtgcc gccgccgatc agcgacacgc cgctgccgaa gcttcccgtg
    32461 cagctcgtgg acatgatgtt gatcgagccg ctcgtcggat gaaccacgaa gatgccctcg
    32521 atgtactggg cgttggcgcc gccattgttg acggtgatct gccccagcgc gccgacggta
    32581 ccggtctggg atgtggtgac gacgatggtc ttgccgttct cgccggcgcc ggtggccgcc
    32641 gcagcgatgg tgaccgtgct tcccgagacg gtcgcggtgt actccggcgt cgatacgaac
    32701 gcgttgatcg cgtcgcggat cagcgtagcg aacgcgctgg tcgagctacc gcccgccgtc
    32761 accggaccgg cgatgatcga gatgccgtcg accgtaaggg tgttgacctt ctcgttcagc
    32821 tcagtctgcg taaccgtcag tgttcccgtc gcggacaccg gcgggatgtt gaccgtgctc
    32881 accgcggtgg tgccgaggtg cttcggccag cggaagttgc cgaccttctt gcgctggcag
    32941 ttgagcagcg tgcctttgtc gctgcaccag cgcgctccgg aatagatgtg cgcgggcgtc
    33001 agcgccgcac ccgcggcaca gttgacgagc tcggggtcgg tgcagaactc cggggcgatg
    33061 tagatgtgcg cgctgggatt gaccgagctc ccgcttacgc aggtcccggc gttgagtgtc
    33121 ggcgtggagc accactgcgc cgtctgcatg cggtagtagt acggcgcgcc gtagcggaac
    33181 ttcacgtggg tatagttgct gacgtagccg ctggtgttgt tgaagccgcg gttgaactcc
    33241 cagttcgggt acgtgtaggc cgagttcacc cggcaattgg tggtatcggt ggcggcgtcg
    33301 gtggtgttcc tgcaccaggc gcgatcaggg tagttcgtta ccaggttctc ggtggtcttg
    33361 ctaatgccgc gctggtcgtc ctgctgctgg ccataggggt cggtcggcac cgcggtccag
    33421 ttgctcgtgt tggttgcgct catgccggtc ttgacgctgc cgtcggaatt gacgccgggc
    33481 cggtaccaga tcgcggggtt gtagtactgc ttgttgaagt ccggcgacat gaacggcgga
    33541 tcgcccgcga tgcagtggtc cgcgcggccg gtgatcgagc ccgcggcgtc gtcgaagtct
    33601 tcggaaaagt tggtaaacgt cgggccggtg tcgccgccgt caaagcacgc ggcggtggtg
    33661 ctcggcgaca cccccgaggg ctcgacgtcg tcgatgtagt ccgggctgta gtcccagtcc
    33721 atggagcccg agtcgtccag gatgaacatg acgttcggct tcacgcccgc cgccgcctgc
    33781 gacagcggct cgttggcgat gtcggtgttg gcggcgcccg ccgtggcggc aaggccgaac
    33841 acgccggcca gggcaagggt cagttggctg taggggaagg cgatgcgcgt tttcatggcg
    33901 atctcacaaa atgcagcgat cagttgacca tcacttggac gtagctcacg gtgttgcgcg
    33961 gcccggtgac ccgggcggtg atgcggtaat agatctgcga ggtgctcggc agggccgcgc
    34021 cgccgtaagt ggcgccgccc tgcgtgccgc cggtggtgcc gcccaggctg gtgcgcacgc
    34081 aattgacgga acccgggttg ccggccaggt cgcacatgcg gtggatgacg tagcgcacct
    34141 ggttgccggc cgggtcggcg ccggcatcga aggaattggc cgcccagtcg aagtcgggcg
    34201 ccgacgggtc ggtggccgtg aaatcgaccc cgctctgcat cgccgccgaa tagcggcccg
    34261 gaacatcgtt gtacaaggtg gccgcggcct gcgcctgaag ccaggcgcgc gccagctcga
    34321 cgccccagtc gccgccgtgc gtggcgttct gcttgaacgc gaggttgccc gcgatgaggt
    34381 tcgcggtgtc cacgccacgg atcaacgcga tgcccgcgag cgacatggcg accaggacga
    34441 tgagcgcgat gaagagcacc gcgccccgct gacgcgcgcg cggaaggggg cgcgtgttca
    34501 tccctgcctc cagatcatgt tgcgcagtgg caccacggtc tcgaatacgc ggtagccgta
    34561 gcagctcgga atgccgccgg gcacggcgaa ggcgccgccg cgccaggtgg gcgctgccgt
    34621 cgtggcgctg caaggatcgc cgacgttctc gggcttcacg aaatgctcgc tccgcgcgag
    34681 cacgccgatg cgtaccgaca gaacctgctg ccactcgacg ccgtttgccg gcgtcacgtt
    34741 gtcgtagctg tccacgatcc cgtcgttcgc cgtatacgca acggcaccga ccgtgccgtt
    34801 gttcacgccg ttgtcttttc cgtactcggc ctgcaggtcg acgatgttgt cgacgatggt
    34861 gaacggtgtg ggcgtatagg ccgggaccac cgtcgacgat gtcgccgaga acagggtcgc
    34921 gagctgcaac gcgttgttgc tgatcgaata gacgttgacg gtcgggcggc cgaggttgaa
    34981 gacctgcgcg ccggcggtga acgcggggag caggctgccg ccgcccgggg ggttgtaggg
    35041 ggccgttacg ccgggaccgt gctgcaggtg gcccgcggcg ggctgcacct gcgtgatctg
    35101 cgtcatggag caggtgccgc cctggacgag cagcaccatg tcgcccggag tgtcggagaa
    35161 cccttgcgga ttgtcgactt ccagctcggc cgagggagac ggcatgctct tgctcagtgt
    35221 cgccggaatc acgcgctcgg tgccgctccc gtacatgatg gtgatcgtgt cgggcccggc
    35281 gccgccgtcg gtgatgacga tgggcgcgag cgtgagcacc ggcaggccgc cgccgggcgg
    35341 gctggagtag tcgccgttgt agaaccactg gatgttggtg cagcccagcg ccgtggacca
    35401 gctgatgccc cagccggcca tgcgcgcctc gcgctcgatc gagaacagcg ccagcgcgcc
    35461 gttggtctgc gcaccgccgg cgctggtggt ggagcgcttg tagttctcgt tcacgaggta
    35521 ggtctgcgtg atgacgacga tgccgatcag gccgatcgcc atcgcgatca ggatctccat
    35581 cagcgacatg cccgccatgc gcgccggcgc ggtgatacgg aagttggact tgagcggcat
    35641 ggtcatgaat cgttgatttg cgcgacgacg gagaattgcc gcagcacgct gtcgctgccg
    35701 ggcagctgcc agaagatcgt cacggttacc tggttgccgg cgacgacgat ggtcggcgag
    35761 ttggtgccgc cggcaacaac gcgcggcagc gtgttcgcca cctgcgcacg ccacgcggtc
    35821 atattcgggt tcgcgccacc gcccgtgtcg taggccgcga ggttggcgcg atcgacccac
    35881 atctggccga tgatctggtt ggcgaggtag gccgcctcgt tgcggtactt cgcctcgacc
    35941 gtattgcgga tcgactgcgc ctgcaggccc atgagcgcga gaatgccgat ggagaagatc
    36001 aggatcccca ccagcgcttc gagcagcgcg acgccggact gggtcttcct cacgggcaga
    36061 tcctcggatc gctcgcgtcg gtgacggccg gatcgcacat gcgtgcctcg ccgccgacgg
    36121 agatgttgat gcgcaagcag cgcatggcgc cgccgtccac gtgctcgcag gtgccgccga
    36181 ccggattgct gatcgccacc tgcgtcatgt tggccgcgcc gccggggctg gtgacacggc
    36241 ccaggccgtt aaagtgcacc acgccggcgg tggccgcagc cagcaccacg ctcgtcgtgc
    36301 cctcgctgcc ggagcgcctc tggatgacgc gcggcgcggc cgtttcggag ggggccgcgt
    36361 cgcaggcacc ggtgacgtcg tcaaggctta ccacccagtc cgtgccggac gtggaaatca
    36421 cgcaggcgga ggtcaggtta ttcaccatct ggaagcgcac gatttggttg cggcgcacgg
    36481 cctcggtgcg tgccagcgtg agccccgcaa gcatggtctc gccagcggtg cggatcttgg
    36541 aattctgcag ccaggtggcc atgttcggca gcccgatcat gatgagcagg cccatgatcg
    36601 ccagcccgat cagcagctcg atcagcgaca cgccggactg tgtggtgcgc gtgatcagca
    36661 ctggctgggc ttcttcgtga tccagcaggc ggcgttggac gtgtagccct tggctgccat
    36721 gctggtggcg ggcgtcacga ccgtcgcgcg ctgattggat tcatcgacgg tgaaggcaat
    36781 gccttcaagt tccgttccca ccatgccggc ggcctgaagg acgaacgtgt tcgcggtcgc
    36841 cgcggccggg caggtgaacg tgaagtactt gacctgcgcg ccgcccgggc cggtcggagc
    36901 gcaaggcccg ccgacgtagg tacgggcgtc ctggaagcgc tgctccatct tcacgcgcag
    36961 gtcggcaagg tgggagtgcg cctcggtgaa cttgctgcgc gtgacatagt ccgtgtacgc
    37021 gggcagcgcc accgcggcaa ggatcgcgac gatggccacc gtgatcatca gttcgatgag
    37081 cgtgaatccc cttgcagaac gcatgagtcc tcctgtctgt gggaaagatg ctaggcgccg
    37141 tcgcgcgctg cggcaaaagc gtgcgaccaa cggccggatt gcgcccgcgg acggcgtgac
    37201 gccccgcagc acggcgtgaa ctgcgtcaca tcgcgggtcc gtcggcgagc ccgtggcgta
    37261 taatgcgcgc cactttgacg cacactgctg gtgtagctca gctggtagag caactgattc
    37321 gtaatcagta ggtcgcccgt tcgagtcggg ccaccagcac caagaaggca cccgatggcc
    37381 gcgcgcatcc tggtggtcga cgacgacgag agcatccgcg acttgcttcg ccttcacctg
    37441 acgtcggccg gctacgaggt ggaggttgcg gccgatgcca tcgcggcggg ctacaaggtc
    37501 ctcaagtcgc cgcccgacct gatcatcacc gacgtcagaa tgccgcacat ggatggcttc
    37561 gagttcgtgg cggcgctgag ggccgacacc accgtgccgc gcattccggt gatcttcctc
    37621 acctcggtcg aggacggcga cgcgcgcgga cgcgagctgg gcgcggtcgg ctatatcacg
    37681 aagccggtgc gctcggaccg cctgctgtcg ctggtggcgc agcacgtgcc gggcggggcg
    37741 attcccatcg gctgacccgc cgcgggctca gctccgcggt gcgcagtcgt gcagcgtcgc
    37801 cccctcgaat tcccgcgcca tcgcctgcag cttttcctgc agtggcggct cgacctcgcg
    37861 cacctggaac cagaccttcg gcacgcgcgt ctcgcgccgg tcgatgatcg cggtgcgcac
    37921 gcccttctcg cgcagcgcct gcaggtggga ccgggccgcc tcttcggtgc tgaagacccc
    37981 gagcgagagc gcccagcgca tcgggcccgc gtcctgcaga atgaagtagt cctccacgcc
    38041 gagcttcttc agctcgccgg ccttgcgcag cgcgttggcg cggctgccct gtggcggcat
    38101 gtgcacccac cagctcgcgg tttcctcgcc gcggtactgc gcgaggcggg cgccgagccc
    38161 gagcggctcg aggcgctgcg cggcgcgcgt ggcctcggcc gggccgaagc tgccccattc
    38221 caggcacgcg agccttgccg gctcgggcgc gggcggcgcc ggcttgggct tggccggcgc
    38281 cggcggcggc gccttgccga gcgcgcgctc gctgacgatg cgcagcttgt gcggctccag
    38341 ttgctgcgca agcggctggc ggtccaccgc gggatcgggc ggcgcgaagc gcatccaggc
    38401 gaaaaagccg aggttcgcgg caagcagcag gagaaacagc agcctcacga ccaggcgatc
    38461 ctggccaggc cttccagaac caggttctcc acatagcggc aggggatctc gagctgctcg
    38521 atgagcgacg gcccggcgcc gcccgagacc aggcattgcg ccccggcgcc cgccacgcgg
    38581 tacatgcgct cgaccgcgcc cgcctgcgcg tgccggcagc cggtttcgat ggcatcgacg
    38641 gtgttgcgcg gcgtgcgcac gaagttgcct tcctgcagcg gcaggcgacc cgtatgctcg
    38701 tgcagcacga agcgcatcag gtccacgccg ggcaggatca ggccgccgag gaactcgccg
    38761 tccgccgaca gcaggtcggc ggtcgtcgcg gtgcccgcct gcaccaccag gcacgggccg
    38821 gtgtgcagcg cgcgtgccgc caccagcgcg gcccagcggt cggcgccgag ctgccccggg
    38881 cgctcgtagc ggctcgtcac gccgcagcgc tcggcctcgc ctttcagcca gaacggctcg
    38941 gcgtcgaaga tgctggtcca gttgacgagc agctggtgcg cgccctcgcc cgcgacattg
    39001 gagatgacga tgcgctccgg gttggcgtgc gtcgccgcaa acggattgat atcgtggttg
    39061 gcggccgcga actcgatcag cgataccgtg gcgaggctcg accaggcgcg gccgtcgtgc
    39121 cagccccact tgacgcgcga gttgccggcg tcgatggcga gaatcatgcc ggggccacgc
    39181 gcccgttgcg caccgtgcgc aggccggtgc gcgtgcgcag ctgcagcgcg ccgtccacgg
    39241 cgaggcccgc ggcgacgccg gcaaagacgc gcccatcggc cagccgcacg cgcagcgccc
    39301 tgcccgcgta ggcgtgcacc gcctcccagt cgccgcgaaa cggctcgaag ccggcggcat
    39361 cgaaggcgcg cagcgcggcg agcacctcgc gcgccagcgc gccgatcacg gcgttgcgcg
    39421 gcggcagcgg ctgcaggccg tcttgaagcg ccgcgatgcc gcgtcgcagc cgcgcggcga
    39481 ggccgggcgc cctgcgccag ttgacgccga taccgatgac cgcggcgctg cccgcgccct
    39541 gggcgcgcgt ctgcaccagg atgccgccga gcttggcgcc gccgagcagc aggtcgttgg
    39601 gccacttgag cgcggtctcg gcggcgccga gcgcgcgcag cgcccgcacg gccgcgaccc
    39661 ccgcgacgat cgacaggccc gagagctcgc gcgccgggcg cgcgagcgcc aggccgagcg
    39721 aaaagagcgc gccgcagccc ggcgggctgt gccagcgccg gccgcggcgg ccgcggccgg
    39781 cggtctgctc gtccgccgcg agcagggcgg ggcgccgcag atcgtccgcg agcagcactg
    39841 tgttggtcga agcgcagcgc gcgacgacgc gcacatcgac gcccgggacg ctgatcgcgc
    39901 ctgcattgag ccgatccatc ggcgctagtg tacggtttag gccgcgccga aggcgaaatg
    39961 gcgtcccgga gcggcgtcga acagcgcccg ggtgtacgcg tggcgcgggg cgccgaacac
    40021 ctccgccgcc gggccgtact cgacgacgcg gccctgcgac atcaccgcca cgtggtcgca
    40081 gatctgcgcc gcgacgcgca ggtcgtgcgt gatgaagagc atcgccaggt tgaagcgctc
    40141 gcggatctcc tcgagcagcg cgagcacctg cgcctgcacc gagacgtcca gcgccgagac
    40201 cgcctcgtcg gcgatcagca actcgggctc catggcgagc gcgcgcgcga tgcagatgcg
    40261 ctggcgctgg ccgccggaga actggtgcgg atagcgctcc agcaccgccg ggtccatgcg
    40321 cacgagttcc atcagcttgc gcgcgcgctc cagcgcctcg gcgcgcggca gtccgtaatt
    40381 gagcgggccc tcgatgatcg cctcgccgac ggtgcggcgc gggttgagcg agcggtacgg
    40441 gtcctggaag acgatctgca ccttgcggcg catctcgcgc aaggcgctca tgggcctggt
    40501 cgcgatgtcc tcgccgtgga tgcggatctc gccgcgcgtc gggtcgatca ggcgcacgat
    40561 gcagcgcgcc acggtcgact tgccggagcc cgattcgccg acgatgccga gcgtctggcc
    40621 gcggcggatc tccagcgtca cgtcatcg
//
LOCUS       DBNHKE010000063        17400 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1068984, whole genome shotgun sequence.
ACCESSION   DBNHKE010000063 DBNHKE010000000
VERSION     DBNHKE010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17400
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1068984"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16O9_04130"
     CDS             complement(<1..512)
                     /locus_tag="AC16O9_04130"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR6515838.1"
                     /translation="MDFAYRNGALCAEQVPLEEIARRFGTPCFVYSRAAIERQYRAFA
                     QALARRTSLVAYSVKANSNLAVLALMARLGAGFDIVSGGELARVIAAGGDPRRVLFSG
                     IGKTEAEIEQALQAGILCLNIESEAELERVDAVAARIGKRAPIALRVNPDIDARTHPY
                     ISTGLRQSKFG"
     gene            complement(520..696)
                     /gene="lptM"
                     /locus_tag="AC16O9_04135"
                     /gene_synonym="yifL"
     CDS             complement(520..696)
                     /gene="lptM"
                     /locus_tag="AC16O9_04135"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: protein motif:HMM:NF047847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR6515839.1"
                     /translation="MRSLALVAAAVLLAAACGQRGPLYLRDQPPPGVTPLKADPYQPV
                     PYPADAERSTEKKK"
     gene            739..1062
                     /gene="cyaY"
                     /locus_tag="AC16O9_04140"
     CDS             739..1062
                     /gene="cyaY"
                     /locus_tag="AC16O9_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HIR6515840.1"
                     /translation="MTDAEFESLADATLAALERAIDRVELDVDVQTKGAGVLEIEFAD
                     GTKLIVNRHTAAREIWVAAKSGGFHFRHAGSAWRDTRDGTELYAALSRLVSSQSATPV
                     ILAPG"
     gene            complement(1059..3479)
                     /locus_tag="AC16O9_04145"
     CDS             complement(1059..3479)
                     /locus_tag="AC16O9_04145"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR6515841.1"
                     /translation="MWLRFLAFPLALLGGLLVAALCVAGLVVALAYPNLPSLDTLTDY
                     QPKVPLRVYTAEGELIGEFGEERRVLVDIKEVPPALTNAIIAAEDERFYQHAGIDYIG
                     VARAAYANLLTGGRRQGASTITMQVARNFFLSSEKTLTRKLYEVLLAVKIERNLTKDQ
                     ILGLYVNQIYLGQRAYGFASAAHTYFGKPLEKLTLAEAAMLAALPKAPSSYNPVANPQ
                     RAKQRQMYVLRRMRELGYVTVDEFESAAAAPIVTRRVVETYGVRAEYVAEMVRQAVYE
                     QYPEDAYSRGFRVYTTIRKADQEAAVLALREGLMEYDRRGGYRGPEAFVELAADADEA
                     DFEEVLAEREDDDDLRVAVVTQASRREVKAVLKTGEAVSITGDGLRFAAPSLQSVPAQ
                     RRIRRGAIVRVRDLGGERWEIVQLPEAEAAFIAMDPTDGAIRALVGGFQYYRSKFNHV
                     TQAWRQAGSAFKPFIYSAALERGFTAATVVPDEPIAIEADVTGSQRWEPKNFDGKFEG
                     PMRLRQALTKSKNMVSIRVLDAIGVKYAQDYVTRFGFDPERHPPYLTMALGSGTVTPW
                     QMARAYAVFANGGYLVQPYFIQKIVDDRGDPLGVARPQRAGDESLRVIDTRNAFIMDS
                     LMQDVARVGTAARAASLGRKDVAGKTGTTNEFIDAWFAGYLPGLVGVSWVGYDQPRSL
                     GRNQTGGLVALPIWVGYMEKVQKAVPAMQRIVPDGVVTADTFSLSVDTLGEVKHLPEY
                     FYREFVPKDDTPPFPLLPVFVPPPDASAIPGVQAVPGELPPGSSPPQFLPPLAPRPAP
                     AAPPGSGY"
     gene            3662..4735
                     /locus_tag="AC16O9_04150"
     CDS             3662..4735
                     /locus_tag="AC16O9_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR6515842.1"
                     /translation="MQLDIFSPKAPPLFGLDISSSSVKMLEIVDAGKGAYRVERYVIE
                     PLPRDATVDGNISNLELVVESVKRGYKRLGTRAKHVAMAVPTGAVITKKIIVPASLRE
                     NELEVQVESEANQYIPFALEEVNLDFQVVGPSPNSPDEQEVMIAATRKEKVEDRVAVA
                     ESAGLKAAVMDIESFAQQTALGLVVNQLPGAGKDLNVAIVDIGANVMNVSVMRNDQSV
                     YTREQAFGGNQLTQDIVARYGMSVEEAESAKRSGGLPDDFETEVLRPFMENLSMEVQR
                     ALQFFFTSTQYHSVDQILLAGGSAVIAGMDEVVQTRTQVPAMVANPFAAMQASPRIQM
                     KKLMIDAPSLIVACGLAMRRFDP"
     gene            4732..5328
                     /locus_tag="AC16O9_04155"
     CDS             4732..5328
                     /locus_tag="AC16O9_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR6515843.1"
                     /translation="MNVRINLLPHREERRKRGRQHFAVLAGLTAALGAAIVGAGYLYI
                     AGLIDNQDRRNAFLRSEIAKLDKEIAEIKKLKDEIAALLARKQIIETLQADRAQTVHL
                     LDQLVRQMPEGVYLRQIRQRGLSVNVQGYSQSNARVSTLMRNIESSPWLSSPQLIEIK
                     AARVGSKRLSEFNMNLQLKRVQLKEAPKDAAKAPANKG"
     gene            5332..5988
                     /locus_tag="AC16O9_04160"
     CDS             5332..5988
                     /locus_tag="AC16O9_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR6515844.1"
                     /translation="MAMAIDFKKIADDFRATNWRDPGTWSAIPKAVVLVAIVTVLPVL
                     GYVGLWQGQLEELETGKAQEAKFKLDYVNKKKQAVNLDLHKQQLREIDTQFGALLKQL
                     PNKSQMDALLVDINQAGLGRGLQFELFKPAGQEIVREFYAELPITVKVVGVYHDMGAF
                     ASDVGQLSRIVTLNDVAIQAAKDGNLVMDVTARTFRYLDEDEIAAQRKAAAGARKGRR
                     "
     gene            5985..6509
                     /locus_tag="AC16O9_04165"
     CDS             5985..6509
                     /locus_tag="AC16O9_04165"
                     /inference="COORDINATES: protein motif:HMM:NF016254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIR6515845.1"
                     /translation="MRRLWILACVAVLAGCEGEQHSDLRKELNEMTKDLRGRVDPLPQ
                     VRPYEPVPYQAENEVDPFRPDRIEVAAAAGTGSAASGKQPDLDRPKEPLEAFPLESIQ
                     MVGSLTQGRDTFGLVKAGPNLYRVKKGNYMGQNFGVITGIDEVQISLKELIQEGSGEW
                     IERASSLQLQEVKK"
     gene            6506..8593
                     /locus_tag="AC16O9_04170"
     CDS             6506..8593
                     /locus_tag="AC16O9_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR6515846.1"
                     /translation="MTVSHVVRAVLHALVLSFAGAGIAHAQANSIEAVDVSQQGGKVV
                     LRITTREALKAPPPSFSVANPARIAFDFAGTTNALGRSSQDIAQGELRSLNLVQAPDK
                     TRLVLNLTRPVAHEVALDGRSVVVTLAAAVVPAGDKVAHFAEGRGESKHAIRDIDFRR
                     GRTGEGRVVVDLSDTTTGIDIRQQGQNIIVDFLKTALPDNLRRRLDVVDFATPVQTIS
                     TFQQGENVRMVIEPRGQWDHNAYQSDTQFVVEVKPVIPDATPKKGQYVGEKLSLNFQN
                     VEVRAVLNVLADFTDLNIITSDSVGGNITLRLKDVPWDQAMDIILQTRGLDSRRNGNV
                     IWIAPRDELATREKLALEATQQIQDLEQTRTEAFQMNYQKAADVAKLLSDRTQPLLSK
                     RGSAVVDTRTNTLFVQDTPTRLEEVRKLLAKIDVPVRQVMIEARIVEASDTFSKNLGV
                     RMGYFEGGVSGRGPGLGISGPNVGAELDNTGQAMGVIQGTPSFTGPGLNSNFRAAGLN
                     SFQPGQISFILFNSSLTRFLNLELSALEADGKGKIISSPRVLTADQVEAVIEQGTEIP
                     YQQATSSGATSVSFRKANLALKVKPQITPDGNVIMTLDVNKDAPGAVTPAGVQISTKH
                     VKTEVLVENGGTVVIGGIFEQTERTDITRVPFFGELPVIGRLFRNDSTTSQKTELLVF
                     VTPRIVNERVTVR"
     gene            8674..9207
                     /locus_tag="AC16O9_04175"
     CDS             8674..9207
                     /locus_tag="AC16O9_04175"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR6515847.1"
                     /translation="MHGGRNVFLVGMMGAGKTTAGRLMARRLKRAFLDSDHEIERRCG
                     VKVPVIFEIEGEAGFRQREVAVIAELTAREDVVLATGGGAVLAEANRRHLAARGTVIY
                     LHAPPPALYERVRQDRNRPLLATANPLARLEELYAVRDPLYREIADIVVDTGRQSVQS
                     LARQLLARLKEEWKLSA"
     gene            9189..10298
                     /gene="aroB"
                     /locus_tag="AC16O9_04180"
     CDS             9189..10298
                     /gene="aroB"
                     /locus_tag="AC16O9_04180"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6515848.1"
                     /translation="METVRVALGPRAYPVHIGTGAIDAEALYHPHLRSGRIAIVTNPV
                     VGPLYLERVKAALSRAGVPTVTEVLVEDGERAKSWQALERVFDALLAARVGRDGLLVA
                     LGGGVVGDLAGFAAAVYQRGIPFLQVPTTLLAQVDSSVGGKTAVNHPRGKNMIGAFHQ
                     PAAVIADVGTLATLPERELRAGIAEVIKHGFALDAALVAWLETHMARLLARDAAALTH
                     VVRRSCELKAQVVAADEREAGARAVLNFGHTFGHAIETGAGYGAWLHGEAVAAGMVMA
                     AELSVRAGFLRAADAERVRRLVRLAGLPAAAPALSPERYIELMAGDKKAAGGRARYVV
                     LQEVGRAALRDDIDERAVREAIVASIDGAPSASAQ"
     gene            10368..15071
                     /locus_tag="AC16O9_04185"
     CDS             10368..15071
                     /locus_tag="AC16O9_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR6515849.1"
                     /translation="MLQSGETLQNAFVNRGLPVNAHLPPDAQGLYDPRHEHDACGVGF
                     VAHIKGKKSHAIVEQGLTVLKNLAHRGAVGWDPKLSDGSGVLIQIPDRLLREDMARQG
                     VKLPPKGQYGVGMVFLPRDPASRQACEYELERAVKDEGQVLLGWRDVPVDNSDLAESA
                     KKLEPVIRQVFIGRGRGVMVTDALERKLYIIRKSAVHAIQSLRLAHARQYYVPSLSAR
                     TLCYKGMLLPNQVGEYYLDLKDARCESALALVHQRFSTNTFPSWDLAHPFRMICHNGE
                     INTVRGNVNWIRARQGAISSPVLGDDLDKIWPLIYDGQSDSASFDNALELLVMGGYSV
                     AHAMMMMIPEAWENHGQMEPARRAFYEYHAAMMEPWDGPASIAFTDGRLIGATLDRNG
                     LRPSRYIVTEDDLVIMGSECGCLPVPEERIVKKWRLQPGKMFLVDLEKGRIVDDAELK
                     ETLSEARPYAEWIERIRVKLDDVGTEKTAPLKSDVPLLDRQQAFAYTQEDIKFVMTPM
                     ALAGEEPIGSMGNDSPLAVLSDKNKTLYHYFKQLFAQVTNPPIDPIREELVTSLVSFI
                     GPKPNLLGIDEMNPPLRLEVSQPVLDFYEMEKIRHIERYTNGKFRSLELDITYPVHWG
                     KDAIEARLASLCAQAEDAVRAGASIIVVSDRLVDRGRVAIPALLALSAIHQHLVGKGL
                     RTSAGLVVETGSAREVHHFALLGGYGAEAVHPYLALETLLAMHPDDSAAGSKAIKNFV
                     KAIGKGLKKVMSKMGISTYMSYTGAQIFEAVGLAKSLVDRYFTGTASNVGGIGLLEVA
                     EEAIRIHHDAFDERDPVLKSMLDAGGEYAWRVRGEEHMWTPEAIAKLQHATRSESYGT
                     YKEYAAIINDQSQRHMTLRGLFEFRLDKVKPVPIAEVEPAAEIVKRFSTGAMSLGSIS
                     TEAHTTLAVAMNRIGGKSNTGEGGEDRRRYAQVKAGESLRSRLGAGRVEKDIALREGD
                     VLKSKIKQVASGRFGVTTEYLASAEQIQIKMAQGAKPGEGGQLPGRKVSEYIAELRYS
                     TPGVELISPPPHHDIYSIEDLAQLIHDLKNANREASISVKLVSEVGVGTVAAGVSKAK
                     SDHVTISGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNRLRGRIAVQVDGQLKT
                     GRDVVIGALLGADEFGFATAPLVVSGCIMMRKCHLNTCPVGVATQDPVLRRKFSGKPE
                     YVVNYMFYVAEEARELMAALGIRKFDELIGRTDLLDMSKGIEHWKAKGLDFSRIFQMP
                     KAPSNVAVYHCEEQDHGLAKALDNRLIELAQPALERGEKVSIDMPIRNINRTVGTMLS
                     YEVAKRYGHEGLADDTIQVRFAGSAGQSFGAFLARGVTLDLIGDTNDYCGKGLSGGRI
                     SVQPSPKFRGEPTENIITGNVVLYGAIAGEAYFRGVAGERFAVRNSGAAAVVEGVGDH
                     GCEYMTGGTVVVLGLTGRNFAAGMSGGIAYVLDIDGGFVQRCNAAMVELEPVLADAEQ
                     QAKGGRELWHHGQTDESNLRRLIEAHLKHTGSKRAQQILDQWATYRQKFVKVFPKEYR
                     RALGELAAAQKKAAA"
     gene            15072..16529
                     /locus_tag="AC16O9_04190"
     CDS             15072..16529
                     /locus_tag="AC16O9_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR6515850.1"
                     /translation="MGKITGFLEYERLQEATEDKEARKKHYREFVLHLTDEQAKVQGA
                     RCMDCGTPFCMSGCPVNNIIPDWNDLVYRGDWQRAIATLHSTNNFPEFTGRVCPAPCE
                     EACVLRINADPVGIKSIEHAIIDRAWEEGWVQPEPAVQKTGKRVAVIGSGPAGLACAQ
                     QLARAGHAVTLYEKNDRIGGLLRYGIPDFKMEKQHIDRRIGQMRAEGVEFRVNHVVGR
                     DPVGAGNAAARAVDPKQLLSEFDAVVLAGGAEVPRDLPVPGRELDGVHFAMDFLPLQN
                     RRVAGDAEVRDLWASGKHVVIIGGGDTGSDCVGTSNRHGAASITQFELLPMPPDPETN
                     PVWPYWPTRLRTSSSHEEGAERDWSVATKAFLGENGKVKALRAARLEWKEGKPVEIPG
                     SEFEMPADLVLLAMGFVSPAKSVLEGLGIATDERGNARADTEGADAYRTSVKKVFAAG
                     DMRRGQSLVVWAIREGRQCARAVDEFLMGGSELPR"
     gene            complement(16531..17223)
                     /locus_tag="AC16O9_04195"
     CDS             complement(16531..17223)
                     /locus_tag="AC16O9_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR6515851.1"
                     /translation="MIDEYLQILLRLLAALAAGGIIGLERSFRGRAAGFRTHALVCIA
                     SSLLMLVTVYEGQWFFSEGGSRVVIDPTRMAQGIMTGIGFLGAGVIVKEGLTVRGLTT
                     AASIWVTAAIGILTGIGFYYAAGLGVVLALGTLSVFRWIEGRMPTELYANFTVRFARD
                     AVMPEPRLRELVAQFNFSVANLNYRLLGDGEQFEYRMVMRTLKADNVRLLAETLNADP
                     GVIEYRISPTSD"
BASE COUNT         3110 a         5652 c         5949 g         2689 t
ORIGIN      
        1 ccgaacttgc tctgccgcaa accggtcgag atgtaaggat gggtcctggc gtcgatgtcg
       61 ggattcacgc gcagcgcgat cggcgcacgc ttgccgatgc gcgcggcgac ggcgtcgacg
      121 cgctcgagct cggcttcaga ctcgatgttc aggcacagga tgccggcctg cagcgcctgc
      181 tcaatctccg cctccgtctt gccgatgcct gaaaacagta cgcggcgcgg atcgccgccc
      241 gcggcgatca cgcgcgccag ttcccccccg gagacgatgt cgaagcccgc gccaagccgc
      301 gccatcagcg cgaggaccgc cagattggag ttcgccttga cggagtacgc gaccagcgac
      361 gtgcggcgcg cgagggcctg cgcgaacgct cggtactggc gttcgatcgc cgcgcgcgaa
      421 tagacgaagc agggcgtgcc gaagcgccgc gcgatttcct cgagcggcac ctgttcggcg
      481 cacagggcgc cgttgcggta cgcgaagtcc atgacggtgc tacttcttct tttcggtgct
      541 gcgctcggcg tccgcgggat acggcaccgg ctggtaggga tcggccttga ggggggttac
      601 gccggggggc ggctggtcgc gcaggtacag cggcccacgc tggccgcagg ccgcggccag
      661 gagcacagcg gcggcaacaa gggcgagaga acgcatccgg cggcggctaa aatgtccatg
      721 ttatcaggga acccccgcat gacagacgcg gaattcgagt cgcttgccga tgccacgctg
      781 gccgcgctgg agcgcgccat cgatcgcgtc gagctcgacg tcgacgtgca gacgaaggga
      841 gcgggtgtgc tggagatcga gttcgccgac ggcaccaagc tgatcgtcaa ccggcacacc
      901 gccgcgcgcg agatctgggt ggcggcgaaa tcgggcggct ttcatttccg ccatgccggc
      961 agtgcgtggc gcgatacgcg cgatggcacc gaactctacg cggcgctgtc gcgcctggtt
     1021 tcgtcgcaaa gcgcgacgcc ggtgatcctg gcaccgggct agtagcccga acccggcggc
     1081 gcggccggtg ccggccgtgg cgccagcggc ggcaggaact ggggcggtga tgatcccggc
     1141 ggcagctcgc ccggcacagc ctggaccccg ggaatcgcgg acgcgtcggg cggcggcacg
     1201 aacaccggca gcaggggaaa cggcggcgta tcgtccttcg gcacgaactc gcgatagaag
     1261 tactcgggca gatgtttgac ctcgcccagc gtatccacgg agaggctgaa cgtgtctgcc
     1321 gtcacgacgc cgtccggcac gatgcgctgc atcgccggca ccgccttctg caccttctcc
     1381 atgtagccca cccagatcgg cagcgcgacc aggccgccgg tttggttgcg gccgagcgag
     1441 cgcggctggt cgtagccgac ccaggacacg ccgaccagtc ccggcaggta tcccgcgaac
     1501 caggcgtcga taaactcatt cgtggtgccg gtcttgccgg cgacgtcctt tcggccgagg
     1561 ctcgccgcgc gcgccgcggt gccgacacgc gccacgtcct gcatcaaact gtccatgatg
     1621 aaggcgttgc gcgtgtcgat gacgcgaagc gattcgtcgc cggcgcgctg tgggcgcgcc
     1681 acgcccagcg gatcgccgcg atcgtcgacg atcttctgga tgaaatacgg ctgcaccagg
     1741 taacccccgt tggcgaatac cgcgtaggcg cgcgccatct gccagggcgt gacggtaccc
     1801 gagccgagcg ccatggtgag gtacggcggg tggcgctcag ggtcgaagcc aaagcgcgtg
     1861 acgtagtcct gcgcatactt gacgccgatc gcgtcgagca cgcggatcga caccatgttc
     1921 ttcgacttgg tgagcgcctg gcgcagacgc attggaccct cgaacttgcc gtcgaagttc
     1981 ttcggctccc agcgctggct gccggtgacg tcggcttcta tggcgatcgg ctcgtcgggc
     2041 accacggtcg ccgccgtgaa gccgcgctcg agcgcggccg aatagatgaa cggcttgaac
     2101 gccgagccgg cctggcgcca ggcctgggtg acgtgattga acttgctgcg gtaatactga
     2161 aaaccgccga ccagtgcgcg gatcgcgcca tcggtcgggt ccatcgcgat gaacgcggcc
     2221 tcggcctccg gcagctgcac gatctcccag cgctcgccgc cgagatcgcg cacgcgcacg
     2281 atcgcgccgc ggcgaatgcg gcgctgcgcc gggacgctct gcagcgaggg cgcggcgaag
     2341 cgcaacccgt cgccggtgat gctgacggct tcgccagtct tcagcactgc ctttacctcg
     2401 cgcctggaag cctgcgttac aacggcgaca cgcagatcgt cgtcgtcctc gcgctcagcg
     2461 agcacttcct cgaagtccgc ctcatccgcg tccgccgcta gctcgacgaa ggcctccggc
     2521 ccgcgatagc cgccgcgccg gtcgtactcc atcaggccct cgcgcagcgc cagcactgcg
     2581 gcctcctggt cggccttgcg gatggtggta tagacgcgaa agccccggct ataggcgtcc
     2641 tcgggatact gctcatagac tgcctggcgc accatctcgg caacgtattc cgcgcgcacg
     2701 ccgtaggtct ccacgactct gcgcgtgacg atcggcgctg cggcggcgct ttcgaactca
     2761 tccaccgtga cgtagcccaa ctcgcgcatg cgccgcagca cgtacatctg gcgctgcttg
     2821 gcgcgctgtg gattggcgac cgggttgtag ctcgagggcg ccttcggcag cgcggcgagc
     2881 atggccgctt cggccagcgt gagcttctcg agcggcttgc cgaaatacgt gtgcgccgcc
     2941 gaggcgaagc cataggcgcg ctgccccaag tagatctggt tgacgtacag gccgaggatc
     3001 tggtccttgg tgaggttgcg ctcgatcttg accgccagca gcacctcgta gagcttgcgc
     3061 gtcagggtct tctcggacga caggaagaag ttgcgcgcca cctgcatggt gatggtgctc
     3121 gcgccctgcc gccgcccgcc ggtcagcagg ttggcgtacg ccgcccgtgc aacgccgatg
     3181 tagtcgatgc ctgcatgctg gtagaagcgc tcgtcttcgg cagcgatgat ggcattcgtc
     3241 agcgccgggg gtacttcctt gatgtcgacc aacacccggc gctcctcgcc gaactcaccg
     3301 atcagctcgc cctcggcggt gtagacgcga agcgggactt tcggttgata gtctgtcagc
     3361 gtgtcgaggg agggcaggtt ggggtaggca agggcgacca ccagcccggc gacgcacaaa
     3421 gccgccacca gcaggccccc caacagggcc agcggaaacg ccaaaaaacg aagccacatc
     3481 aagggttaga cagtcatcgc ggcactggta cggccgcagt tgctggggtg gagaatcggg
     3541 ggcattatag ccctccccca gagggtgacg gattcgctag acacaggggg gagttgttgc
     3601 tagcatttaa cgcaaattct acgtatcgtg cctaagggcc gatacaaaaa gggaaataac
     3661 gatgcaactg gatatcttca gcccgaaggc gccgccgttg ttcgggctgg acatcagctc
     3721 atcgtcggtg aagatgctcg agatcgtgga cgcgggcaag ggtgcctatc gcgtcgagcg
     3781 ctacgtgatc gagcccctgc cccgggacgc taccgttgac ggcaacatca gcaacttgga
     3841 gttggtggtc gagtcggtca agcgcggcta caagcgtctg ggtacgcgcg ccaagcacgt
     3901 cgcgatggcg gtgcccaccg gcgccgtgat taccaagaag atcatcgtcc cggcgagcct
     3961 gcgcgagaac gagctcgagg tacaggtgga atcggaggcg aatcaataca tcccgtttgc
     4021 gctcgaggaa gtgaatctgg acttccaggt cgtcggccca tcgccgaact cgccggacga
     4081 gcaggaggtg atgatcgccg cgacacgcaa ggagaaggtg gaggaccgcg tcgccgtggc
     4141 ggagtccgcc gggcttaagg cggcggtcat ggatatcgag tcgtttgcgc agcagaccgc
     4201 gctcgggctg gtggtgaacc agcttcccgg cgccggcaag gacctgaacg tcgctatcgt
     4261 cgacatcggc gccaacgtga tgaacgtgag cgtgatgcga aacgaccaat cggtgtacac
     4321 gcgcgagcag gccttcggcg gcaaccagct gacccaggac atcgtcgcgc gctacggcat
     4381 gagcgtcgag gaagcggaga gcgccaagcg ttccggcggc ctgcccgatg acttcgaaac
     4441 cgaggtgctg cggccgttca tggagaacct gtccatggag gttcagcgtg cactgcagtt
     4501 cttcttcacc tcgacgcagt accacagcgt cgatcagatc ctgctcgccg gcggctcggc
     4561 cgtcatcgca ggcatggatg aagtggtgca gacgcgcacg caggtcccgg ccatggtggc
     4621 caacccgttc gccgcgatgc aggcttcgcc ccgcatccag atgaagaagc taatgatcga
     4681 cgcgccgtcg ctgatcgtcg cctgtggcct cgccatgcgc aggttcgacc catgaacgtg
     4741 cgcatcaacc tgctgccgca ccgagaggag cggcgcaagc gcggccgcca gcatttcgcg
     4801 gtgctcgccg ggctcaccgc tgcccttggc gcggcgatcg tcggcgcggg ctacctgtac
     4861 atcgccggcc tgatcgacaa tcaggaccgg cgtaacgcgt tcctgcgctc cgagatcgcg
     4921 aaactggaca aggaaatcgc cgagatcaag aagctgaagg acgaaatcgc ggcgctgctc
     4981 gcgcgcaagc agatcatcga gacgctgcag gcggaccgcg cgcagaccgt gcatctgctc
     5041 gaccagctgg tgcggcagat gcccgagggc gtgtacctgc gccagatccg gcagcgcggc
     5101 ctgtcggtca acgtgcaggg ctactcgcaa tccaacgcgc gcgtgtccac gctgatgcgc
     5161 aacatcgagt cctcaccctg gctctccagc ccgcaactga tcgagatcaa ggctgcgcgc
     5221 gtgggctcga agcgcctgtc cgaattcaat atgaacctgc agttgaagcg cgtccagctg
     5281 aaggaagcac ccaaggacgc ggccaaggcg ccggcgaaca aggggtaggc catggcgatg
     5341 gcgatcgatt tcaagaagat tgcggatgac ttcagggcca ccaactggcg cgatccgggc
     5401 acgtggtccg cgattcccaa ggcggtggtg ctggtggcga tcgtgaccgt gctgccggtg
     5461 ctgggctacg tcggcctgtg gcagggccag ctcgaggagc tggagaccgg caaggcccag
     5521 gaggcgaaat tcaagctcga ttacgtgaac aagaagaagc aggcggtgaa tctcgacctg
     5581 cacaagcagc agctcaggga gatcgatacg cagttcggcg cgcttctcaa gcagctgccc
     5641 aacaagtcgc agatggatgc gctgctcgtc gacatcaacc aggcgggcct cggccgcggc
     5701 ctgcagttcg agctgttcaa gccggcgggc caggaaatcg tgcgcgagtt ctatgcagaa
     5761 ttgccgatca ccgtgaaagt ggtcggcgtg taccacgaca tgggggcgtt cgcgagcgac
     5821 gtcggccagc tctcgcgcat cgtcacgctg aacgacgtcg caatccaggc cgccaaggac
     5881 ggcaacctgg tgatggacgt caccgcgcgc acgttccgct acctggacga ggacgagatc
     5941 gccgcgcagc gcaaggccgc cgccggcgcg cgaaaagggc ggcgatgagg cgcctatgga
     6001 tcctggcctg cgtcgcggtg ctcgccggct gcgagggcga gcagcacagc gacctgcgca
     6061 aggagctgaa cgagatgacc aaggacctgc gtggccgggt ggatcccctg ccgcaggtgc
     6121 ggccctacga gcccgtgccc taccaagcgg agaacgaggt cgacccgttc cgtccggatc
     6181 gcatcgaggt cgcggccgcc gcaggcaccg ggtcggcggc gagcggcaag cagccggacc
     6241 tcgaccgtcc caaagagccg ctcgaggcgt ttcctctcga gtccatccag atggtgggct
     6301 cgctcacgca gggccgcgac acgttcggcc tggtgaaggc cggccccaac ctgtaccgcg
     6361 taaagaaggg caactacatg gggcagaact tcggcgtcat taccggcatc gatgaagtgc
     6421 agatcagcct gaaagagctg atccaggaag gcagcgggga gtggatcgag cgggcgagct
     6481 cgctgcaact gcaggaggtc aagaaatgac agtcagccac gtcgtgcgcg ccgtcttgca
     6541 tgcgctcgtt ctcagcttcg cgggcgcggg catcgcccat gcgcaggcca atagcatcga
     6601 ggcggtcgac gtctcgcagc agggtggcaa ggtggtgctg cgcatcacca cccgggaggc
     6661 cctgaaggcc ccgccgccca gcttcagcgt cgccaacccg gcgcgcattg cgttcgattt
     6721 cgccggcacg accaacgcgc tcggccgctc gagccaggac atcgcacagg gcgagctgcg
     6781 ctcgctcaat ctggtgcagg cacccgacaa gacgcgcctg gtgctcaatc tgacccgtcc
     6841 ggtcgcacac gaagtggcgc tcgacggccg cagcgtggtc gtgacgctcg ctgcggcggt
     6901 ggtaccggcg ggcgacaagg tggcgcactt cgccgagggg cgcggcgagt ccaagcacgc
     6961 gatccgcgac atcgatttcc ggcgtgggcg caccggtgag ggccgcgtcg tggtggacct
     7021 gtcggacacc acgaccggca tcgacatccg ccagcagggg cagaacatca tcgtcgattt
     7081 cctgaaaacg gccctgccgg acaacctgcg ccgccgcctt gacgtggtgg acttcgccac
     7141 gccggtgcag accatcagca ccttccagca gggcgagaac gtgcgcatgg tgatcgagcc
     7201 gcgcgggcaa tgggaccaca acgcctacca gtccgatacg cagttcgtgg tggaagtgaa
     7261 gccggtgatc cccgacgcga cgccgaagaa aggccagtac gtcggcgaga agctctcgct
     7321 caacttccag aacgtggaag tgcgcgccgt actgaacgtg ctcgcggatt tcaccgacct
     7381 caacatcatc accagcgatt ccgttggcgg caacatcacg ctgcgcctga aggacgtgcc
     7441 ctgggaccag gcgatggaca tcatccttca gacccgcggc ctcgacagcc ggcgcaacgg
     7501 caacgtcatc tggatcgcac cgcgcgacga gctggcaacg cgcgagaagc tcgcgctgga
     7561 agccacgcag cagatccagg acctcgagca gacgcgcacc gaggctttcc agatgaacta
     7621 ccagaaggcg gccgacgtgg cgaagctgct ctcggaccgc acgcagccgc tcctgtccaa
     7681 gcgcggcagc gcggtggtcg acacccgcac caacacgctt ttcgtgcagg acacgccgac
     7741 gcggctggag gaagtgagaa agctcctcgc caagatcgac gtcccggtgc gccaggtgat
     7801 gatcgaggcg cgcatcgtcg aggcgagcga cacgttcagc aagaacctcg gcgtgcgcat
     7861 gggctatttc gaaggcggtg tgtcgggccg cggcccgggg ctcggaatca gcggccccaa
     7921 cgtcggcgcg gagctggaca ataccggcca ggcgatgggc gtcattcagg gcacgccctc
     7981 cttcacgggc ccgggcctca attcgaactt ccgcgccgcc ggcctgaact cgtttcagcc
     8041 gggccagatc tcgttcatcc tgttcaacag ttcgctcacg cgcttcctca acctcgagct
     8101 ctcggcgctg gaggccgacg gcaagggcaa gatcatttcc agcccgcgcg tgctgaccgc
     8161 ggaccaggtc gaggcggtaa tcgagcaggg caccgagatc ccctaccagc aggcgacctc
     8221 gtccggcgcg acgagcgtat ccttccgcaa ggcgaatctc gcgctgaagg tgaagccgca
     8281 gatcacaccc gacggcaacg tcatcatgac gctcgacgtg aacaaggacg ccccgggcgc
     8341 ggtcaccccg gcgggcgtgc agatcagcac caagcacgtc aagaccgaag tgctggtcga
     8401 gaacggcggc accgtggtga tcggcggcat cttcgagcag accgagcgta ccgacatcac
     8461 acgggtaccg tttttcggcg agctgccggt catcgggcgg cttttccgca acgactctac
     8521 gacgtcacag aagaccgagt tgctcgtatt cgtgacgccg cgcatcgtca acgagcgggt
     8581 gacggtgcgc tgacagtttg ccaggtctcc ttctgggaca cgcggccggg ctcgcccggc
     8641 cgtttttgtt tgtggcgtcc gtacaatagg cgcatgcacg gcgggcgcaa cgtattcctc
     8701 gtgggcatga tgggcgcggg caagaccacc gcgggccggc tcatggcgcg ccggctaaag
     8761 cgcgccttcc tcgacagcga ccacgagatc gagcgccgct gcggcgtgaa ggtgcccgtg
     8821 atcttcgaaa tcgagggcga ggcgggcttt cgccagcgcg aagtggcggt gatcgcggag
     8881 ctcaccgcgc gcgaagacgt ggtgctcgcc accggaggcg gggccgtgct cgcggaggcc
     8941 aatcgccgcc atctcgccgc gcgcggcacg gtgatttacc tgcatgcgcc gcccccggcg
     9001 ctctatgagc gcgtgcgtca ggaccgcaac cggccgctgc tcgccaccgc caacccgctg
     9061 gcgcgcctcg aggagctgta tgcagtgcgc gatcccctgt accgagagat cgccgacatc
     9121 gtcgtcgaca cgggccggca gagcgtgcag agcctggcac gccagctgct tgcccgccta
     9181 aaggaagaat ggaaactgtc cgcgtagcgc ttggcccgcg cgcctacccg gtgcacatcg
     9241 gcaccggcgc gatcgacgcc gaggccctct atcacccgca tctgcgctcc gggcgtattg
     9301 cgatcgtcac caatccggtg gtcgggccgc tgtacctcga gcgcgtgaag gcagcgctgt
     9361 cgcgcgccgg tgtcccgacg gtcaccgagg tcctggtcga ggacggtgag cgggcgaaga
     9421 gctggcaggc gctggagcgc gtgttcgacg cgctgctcgc cgcgcgcgtc ggacgcgacg
     9481 gcctgctcgt cgccctgggc ggtggcgtag tcggcgatct ggccggcttt gccgcggcgg
     9541 tctatcagcg cggcatccca tttctccagg tgccgacgac gctcctggcc caggtcgact
     9601 cctccgtggg cggcaagacc gcggtgaacc atccccgcgg caaaaacatg atcggcgcat
     9661 tccaccagcc cgccgccgtg atcgccgacg tcggcacgct cgccaccctg cccgaacgcg
     9721 aactgcgcgc gggtattgct gaggtgatca agcatggttt cgccctcgac gccgcgctcg
     9781 tcgcgtggct ggagacccac atggcgcggc tgctcgcgcg cgacgccgcg gcgctcacgc
     9841 acgtggtgcg ccgttcctgc gagctaaagg cgcaggtggt ggcggcggac gagcgcgagg
     9901 ccggtgcgcg cgccgtgctc aatttcgggc acacattcgg ccacgccatc gagaccggcg
     9961 ccggctacgg cgcctggctg cacggcgagg cggtggcggc cggcatggtc atggcggccg
    10021 agctttcggt gcgcgctggc tttctgcgcg cggccgacgc cgagcgcgtg cgccgcctcg
    10081 tgcgcctggc cgggttgccc gcggcggcac cggcgctctc gcccgagcgc tacatcgagc
    10141 tgatggcggg cgacaagaag gcggccggcg ggcgcgcccg ctacgtcgtg ctgcaagagg
    10201 tgggccgcgc cgcgcttcgt gacgacatcg acgagcgcgc ggtgcgcgag gcgattgtcg
    10261 cgagcatcga cggcgcgccg tccgcttcgg cgcagtaagg cgccagggcc ccgaaactct
    10321 tgttggtgca agaccgtgca ggtatattcc acaatccggc cgcagaaatg ctgcagagcg
    10381 gcgaaacgct ccaaaatgca tttgtgaaca gagggttacc tgtgaacgcg cacctcccgc
    10441 ccgacgcgca gggcttgtac gatccgcggc acgagcacga cgcgtgcggc gtaggctttg
    10501 tcgcgcacat caagggcaag aagtcgcacg cgatcgtcga gcagggactc acggtgctga
    10561 agaaccttgc gcatcggggc gcggtcggct gggatccgaa gctctcggac ggctcgggcg
    10621 tgctgatcca gatacccgat cggctgttgc gcgaggacat ggcgcgccag ggggtgaagc
    10681 tgccacccaa gggtcagtac ggcgtcggca tggtgttcct gccgcgcgac ccggcgagcc
    10741 gccaggcgtg cgagtacgag ctcgagcgcg cggtgaagga cgaaggccag gtgctgctcg
    10801 gctggcgcga cgtgcccgtg gacaattccg acctcgccga gtcggcgaag aagctcgaac
    10861 cggtgatccg ccaagtgttc atcggccgcg gccgcggcgt catggtcacc gatgcgctgg
    10921 agcgcaagct ctacatcatc cgcaagagcg cggtgcatgc catccagtcg ctgcggctgg
    10981 cacatgcccg gcagtactac gtgccgtcgc tgtcggcgcg cacgctgtgc tacaagggca
    11041 tgctcctccc gaaccaggtc ggcgagtact acctcgacct caaggacgcg cgctgcgaat
    11101 ccgcgcttgc gctggtgcac cagcgctttt cgaccaacac cttcccgtcg tgggatctgg
    11161 cgcacccgtt ccgcatgatc tgccacaacg gcgagatcaa caccgtgcgc ggcaacgtca
    11221 actggatccg cgcacgccaa ggggcgattt ccagcccggt gctcggcgac gacctcgaca
    11281 agatctggcc gctcatttac gacggccaat ccgactcggc ctctttcgac aacgcgctcg
    11341 agctcctggt gatgggcggt tattccgtcg cccacgccat gatgatgatg atccccgagg
    11401 cctgggagaa ccacgggcag atggaacccg cgcggcgcgc gttctacgag taccacgcgg
    11461 cgatgatgga gccttgggat ggtccggcgt cgatcgcgtt caccgacggg cgcctcatcg
    11521 gcgctacgct cgatcgcaac ggcctgcgcc cgtcgcgcta catcgtcacc gaggacgatc
    11581 tcgtcatcat gggctcggaa tgcggctgcc tgccggtgcc cgaggagaga atcgtcaaga
    11641 aatggcgcct gcagccgggc aagatgtttc tcgtcgacct ggaaaaaggc cgcatcgtcg
    11701 acgacgcgga attgaaggag acgctgtcgg aagcgcggcc ctacgccgag tggatcgagc
    11761 gcattcgcgt caagctcgac gatgtcggga ccgagaagac cgcgccgctg aagagcgacg
    11821 tgccgctgct cgaccgccag caggccttcg cctacacgca ggaagacatc aagttcgtga
    11881 tgacgccgat ggcgctcgcc ggcgaagagc cgatcgggtc gatgggcaat gattcgcccc
    11941 tcgcggtgct ctcggacaag aacaagacgc tgtaccacta tttcaagcag ctcttcgcgc
    12001 aggtcacgaa cccgccgatc gatccgatcc gcgaggagct ggtgacctcg ctggtgtcgt
    12061 tcatcgggcc gaagccgaac ctgctcggca tcgacgaaat gaacccgccg ctgcgcctcg
    12121 aagtgtcgca gccagtgctc gatttctacg agatggagaa aatccggcac atcgagcgct
    12181 atacgaacgg caagttccgc tcgctcgagc tcgacattac ctacccggtg cactggggca
    12241 aggacgccat cgaggcgcga ctggcttcgc tctgcgcgca agccgaggac gcggtgcgcg
    12301 cgggcgcctc gatcatcgtg gtgtcggacc ggctggtcga tcgagggcgg gtggcaatcc
    12361 cggcgctgct cgcgctttcg gcgatccacc agcacctggt ggggaagggc ctgcgcacga
    12421 gcgccggcct ggtcgtggaa acgggctcgg cgcgcgaggt gcaccacttt gcgctgctcg
    12481 gcggctacgg cgcggaggcc gtgcacccgt accttgcgct ggagacgctt ctcgccatgc
    12541 acccggatga ctcggcggcg ggcagcaagg cgatcaagaa tttcgtcaag gcgatcggca
    12601 aggggctgaa gaaggtgatg tcgaagatgg gcatttccac ctacatgtcc tataccggcg
    12661 cgcagatctt cgaggcggtg ggcctggcga agtcgctcgt cgaccgctac ttcaccggta
    12721 ccgcgtcgaa tgtcggcggc atcggactgc tcgaggtcgc cgaagaggcg atacgcatcc
    12781 accacgacgc tttcgacgag cgcgacccgg tgctgaaatc gatgctcgat gcgggcggcg
    12841 aatacgcctg gcgcgtgcgc ggcgaggagc acatgtggac gcccgaagca atcgccaagc
    12901 tgcagcacgc cacgcggagc gagtcctacg gcacctataa ggaatacgct gcgatcatca
    12961 atgaccagtc ccagcgccac atgacgttgc gcgggctgtt cgagttccgc ctcgacaagg
    13021 tgaagccggt gccgattgcc gaggtcgagc cggccgcgga gatcgtgaag cgcttctcca
    13081 ccggcgccat gtcgctcggc tcgatctcga ccgaggcgca cacgacgctc gccgtggcca
    13141 tgaaccgcat tggcggcaag tcgaataccg gcgaaggcgg tgaggaccgc aggcgttacg
    13201 cacaggtgaa agcgggtgag tcgctgcgca gccgcctcgg cgcgggccgt gtggagaagg
    13261 acatcgcgct gcgcgagggc gacgtcctga agtccaagat caagcaggtg gcttcgggcc
    13321 gcttcggcgt caccaccgag tatctggcga gcgccgagca gatccagatc aagatggcgc
    13381 agggtgcaaa gcccggcgag ggcggccaac ttccgggccg caaggtctcc gagtacatcg
    13441 ccgaactgcg ctactcgacc ccgggcgtgg agctgatcag cccgccgccg caccacgaca
    13501 tctactcgat cgaggacctg gcgcagctga tccacgacct gaagaacgcc aatcgcgagg
    13561 catcgatctc ggtgaagctc gtgtccgaag tgggcgtcgg cacggtcgcg gccggggttt
    13621 cgaaggcgaa gtcggaccac gtcacgatct ccggacacga tggcggcacc ggcgcctcgc
    13681 cctggagctc gatcaagcac gcgggcacgc cctgggagct gggacttgcc gaaacgcagc
    13741 agacgctggt gctgaaccgc ctgcgcggca ggatcgcagt gcaggtcgac gggcagctga
    13801 agaccggtcg cgacgtggtg atcggcgcgt tgctcggggc cgacgaattc ggcttcgcta
    13861 ctgcgccgct ggtggtgagc ggttgcatca tgatgcgcaa gtgccacctc aacacctgcc
    13921 cggtgggcgt cgccacgcag gacccggtgc tgcggcgcaa gttctcgggc aagcccgagt
    13981 acgtggtcaa ttacatgttc tacgtcgccg aggaggcgcg cgagctgatg gcggcgctcg
    14041 gcatccgcaa gttcgacgag ctgatcggcc gcaccgatct cctcgacatg agcaagggca
    14101 tcgagcactg gaaggcgaag gggctcgatt tttcgcgcat cttccagatg ccgaaggcgc
    14161 cgtcgaacgt cgccgtgtat cactgcgaag agcaggatca tggcctcgcc aaggcgctcg
    14221 acaaccgcct tatcgagctg gcgcagccgg cgctcgagcg cggcgagaag gtgtcgatcg
    14281 acatgccgat ccgcaacatc aaccgcaccg tcggcacgat gctctcctac gaggtggcga
    14341 agcgctacgg ccacgagggc ctcgccgacg atacgatcca ggtgcgcttc gccggctcgg
    14401 ccggacagag cttcggcgcg ttcctggcgc gcggcgtgac gctcgatctc atcggcgaca
    14461 ccaatgacta ctgcggcaaa gggctctcgg gcgggcgcat cagcgtgcag ccctcgccga
    14521 agttccgcgg cgagccgacc gagaacatca tcaccggcaa cgtggtgctg tacggtgcga
    14581 tcgccggcga ggcgtatttc cgtggcgtcg cgggcgagcg cttcgcggtg cggaactccg
    14641 gcgccgccgc ggtggtggag ggcgtgggcg accacggctg cgagtacatg accggcggca
    14701 cggtggtggt gctcggcctc acgggccgca acttcgctgc gggcatgtcc ggcggcatcg
    14761 cctatgtgct cgacatcgac ggcggcttcg tgcagcgctg caatgcggcg atggtcgaac
    14821 tggagcccgt gctggccgat gcggagcagc aagccaaggg tgggcgcgag ttgtggcacc
    14881 acggacagac cgacgaatcg aacctgcggc gcctgatcga ggcgcacctc aagcacaccg
    14941 gcagcaagcg cgcgcagcag atcctcgacc agtgggcgac ctatcggcag aagttcgtca
    15001 aggtgttccc caaggagtac cggcgcgcgc tgggcgagct cgccgcggca cagaagaagg
    15061 cggcggcgta gatggggaag atcacaggtt tcctggagta cgagcgactc caggaggcga
    15121 ccgaggacaa ggaagcccgc aagaagcact atcgcgagtt cgtcctgcat ctcaccgacg
    15181 agcaagcgaa ggtacagggc gcgcgctgca tggactgcgg tacgccgttc tgcatgtcgg
    15241 gatgcccggt gaacaacatc atccccgact ggaacgacct cgtgtaccgc ggcgactggc
    15301 agcgcgccat cgccacgctg cattccacca acaactttcc cgagttcacc ggccgcgtgt
    15361 gcccggcgcc ctgcgaagaa gcctgcgtgc tgcgcatcaa cgccgacccg gtcggcatca
    15421 agtcgatcga gcacgccatc atcgaccggg cgtgggagga aggctgggtg cagccggagc
    15481 ctgcggtgca aaagaccggc aagcgcgtgg cggtcatcgg ctcggggccc gcggggctcg
    15541 cctgcgccca gcagctcgca cgggccggac acgcggtgac gctctacgag aagaacgacc
    15601 ggattggcgg gctgctgcgc tacgggatcc cggacttcaa gatggaaaag cagcacatcg
    15661 accgccgcat cgggcagatg cgggcggagg gcgtcgagtt tcgcgtcaac cacgtggtgg
    15721 ggcgcgatcc ggtcggcgcg ggcaacgcgg cggccagggc ggtggacccg aagcagctgc
    15781 tgagcgagtt cgacgccgtg gtgctcgcgg gcggggcgga agtgccgcgc gacctgccgg
    15841 tgccggggcg cgagctcgac ggtgtgcatt tcgccatgga cttcctgccg ctgcagaacc
    15901 ggcgcgtggc cggcgacgcg gaagtcaggg acttgtgggc gagcggcaag catgtggtga
    15961 tcatcggcgg cggcgacacc ggctcggact gcgtcggcac gtcgaaccgt cacggcgccg
    16021 cgtcgataac gcagttcgag ctgctgccga tgccgcccga ccccgagacg aatcccgtgt
    16081 ggccgtactg gccgacacgg ctgcgcacct cgtcctcgca cgaagaaggc gcggagcgcg
    16141 actggtcggt ggccaccaag gcgtttctcg gcgagaacgg caaggtcaag gcgctgcgcg
    16201 ctgcgcggct ggagtggaag gaaggcaagc cggtggaaat tcccggctcg gagttcgaga
    16261 tgcccgccga cctcgtgctg ctcgcgatgg gcttcgtgtc gccggcgaaa tccgtgctcg
    16321 aagggctcgg catcgccacg gacgagcgtg gcaacgcgcg cgccgatacc gaaggggcgg
    16381 acgcctatcg cacttcggta aaaaaggtgt tcgccgcggg ggacatgcgc cgcggccagt
    16441 cgctcgtcgt ctgggcgata cgcgaaggac ggcagtgcgc gcgcgccgtg gacgagttcc
    16501 tgatgggcgg gtcggaactg ccgcgttagc tcagtcgctc gtcggcgaga tccggtactc
    16561 gattacgccg gggtcggcgt tcagggtctc ggccagcagg cgcacgttgt ccgccttgag
    16621 cgtgcgcatc accatgcggt actcgaactg ctcgccgtcg cccagcaggc ggtagttgag
    16681 attggcgacg gaaaagttga actgcgccac cagctcgcgc aggcgcggct caggcatcac
    16741 ggcatcgcgc gcaaagcgta ccgtgaagtt ggcgtacaac tcggtcggca tgcggccctc
    16801 gatccagcgg aacaccgaca gcgtgccgag cgcgagcacc accccgaggc ccgccgcgta
    16861 gtaaaagccg ataccggtga gaatgccgat cgccgcagtg acccagatgg aggcggcggt
    16921 ggtgaggccg cgcaccgtga gcccttcctt cacgatcacg ccggcgccga ggaagccgat
    16981 cccggtcatg atgccctgtg ccatgcgtgt cggatcgatg accacgcgcg agccgccttc
    17041 cgagaagaac cactgccctt cgtagaccgt gacgagcatc agcaggctcg acgcaatgca
    17101 gaccagcgca tgcgtgcgaa agccggccgc gcggccgcga aagctgcgct cgaggccgat
    17161 gatgccgccg gcggcaagcg cggccagcag ccgcaggagt atctgcaggt attcgtcgat
    17221 caaggtctga agcgccgtcc ggtggcagtc atgttacagc ggttttacat gatctttcaa
    17281 tgagttcgac cgtttccggg tgctagaatc cgcgccatga gcctcgccgt gatcgtgctt
    17341 gccgcgggcc aaggcaagcg catgcactcg gccttgccca aggtgctgca tccgctggcg
//
LOCUS       DBNHKE010000064         3224 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_698475, whole genome shotgun sequence.
ACCESSION   DBNHKE010000064 DBNHKE010000000
VERSION     DBNHKE010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_698475"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC16O9_04200"
     CDS             complement(<1..710)
                     /locus_tag="AC16O9_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6515852.1"
                     /translation="MRVAWLQDLRLTDLPQVGGKNASLGEMIGALKGAGIRVPGGFAT
                     TADAYREFLKADGLDQRIAKRIADLKDGDVEALARCGAEIRGWIEKAPFPKALEQDIR
                     KYYQEVEKQYSSDASFAVRSSATAEDLPDASFAGQQETFLNVRGVDNVLQAIRHVFAS
                     LYNDRAISYRAHRGFEHGDVALSAAVQQMVRSDRGASGVMFTLDTESGFRDVVFITAA
                     YGLGEMVVQGAVNPDEFYV"
     gene            731..1555
                     /gene="ppsR"
                     /locus_tag="AC16O9_04205"
     CDS             731..1555
                     /gene="ppsR"
                     /locus_tag="AC16O9_04205"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HIR6515853.1"
                     /translation="MPQRRTVFFISDGTGITAQMLGHSLLTQFEGMEFHQVTVPFVNS
                     QDQAEDCRARIDEESRHGNGLPIVFSTLVNGELRAVVRKASALFVDFFETFIDPLEAA
                     LGVQSTHTIGRSHSALDKPGYSHRMEAINFAMSHDDGASNRDLDQADVILVGVSRCGK
                     TPTSLYLALQFGVKAANYPLIPEDFARMQLPPALAPCKARLYGLSISPERLREIRQAR
                     RPDSRYADLDNCRYEVEQAERLMRREGIRWINSTAKSIEEIATNILRELRLERPMY"
     gene            complement(1530..2318)
                     /locus_tag="AC16O9_04210"
     CDS             complement(1530..2318)
                     /locus_tag="AC16O9_04210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6515854.1"
                     /translation="MSLLRSRDNPRVRRWHALAHDGRSRRAERSALIEGAHLLAAYLD
                     SGARPKAVLVSASGQANAEIAALARRAGPAPVTLPDPVFRWVADAVTPAGLAAEIELP
                     PAAPVAQATHAVFLDGVQDAGNVGAILRSAAAFGVDTAVLGRGCADPWSPKVLRAAMG
                     GHFALGITVVDELAGALAQFPGTRVCAAAAGGDAPATLDLTASIGWILGAEGQGVSPA
                     AAAQASHRVSIPLAPDVESLNVAAAAAILLYERARQLSTSGARA"
     gene            complement(2315..2896)
                     /gene="rnhB"
                     /locus_tag="AC16O9_04215"
     CDS             complement(2315..2896)
                     /gene="rnhB"
                     /locus_tag="AC16O9_04215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6515855.1"
                     /translation="MRAVPERLICGVDEAGRGPIAGPVYAAAVLLDPRRRIAGLRDSK
                     LLPPGRREALAEAIRGRALAWAVASASVEEIDRLNILQASLLAMRRAVEALAVAPQEA
                     WIDGNQCPLLGCAVRAVIGGDRRVRQISAASILAKTARDAEMLRLHERFPHYGLDQHK
                     GYPTPEHLARLAQFGACEIHRRSFAPVRQVLGA"
     gene            complement(2880..>3224)
                     /locus_tag="AC16O9_04220"
     CDS             complement(2880..>3224)
                     /locus_tag="AC16O9_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR6515856.1"
                     /translation="VASGTATLEAALHKTPMVIVYRQSPLSWALQRQMLYLPYIGMPN
                     ILAGERLVPELIQGRATPAALSAALIDLLRDTDLQERQVRRFREMHALLRQGNAQKAA
                     DAVLGVLDAGRA"
BASE COUNT          511 a         1156 c         1073 g          484 t
ORIGIN      
        1 acgtaaaact cgtccgggtt gacggcgccc tgcaccacca tctcgcccag gccgtaggcg
       61 gcggtaatga atacgacgtc gcgaaagccc gattcggtat ccagcgtgaa catgacacca
      121 ctcgcgccgc ggtcgctgcg caccatctgc tgtacggccg ccgacagcgc gacatcgccg
      181 tgctcgaagc cccggtgggc acggtaggaa atggcgcgat cgttgtagag cgaggcgaat
      241 acgtgccgga tggcctgcag cacgttgtcg acgccgcgca cattcaggaa ggtctcctgc
      301 tgcccggcga acgaggcgtc cggcagatct tcggctgtgg cggaagacct gacagcaaac
      361 gaggcatcac ttgaatattg cttttcaact tcttgataat acttgcgaat atcttgctcc
      421 aaagccttcg gaaagggcgc cttctcgatc catccacgga tctccgcccc acagcgcgcc
      481 agcgcctcga cgtcgccgtc cttcaggtcc gcgatgcgct tggcgatgcg ctggtcgagt
      541 ccgtcggcct tcaggaactc gcggtaggcg tcggcggtcg tggcgaaacc gccgggcaca
      601 cgaatgcccg cgcccttgag ggcaccgatc atctcgccca gcgacgcgtt cttgccgccg
      661 acctgcggca agtcggtcag gcgcaaatcc tgcagccacg cgactctcat ttacctgcta
      721 ttttagccgc atgccgcagc gccgcaccgt gttcttcatc tctgacggca ccggtatcac
      781 ggcgcagatg ctcggtcaca gcctgctcac acagttcgag ggcatggagt ttcaccaggt
      841 caccgtgccc ttcgtcaact cgcaggacca ggccgaggac tgccgcgcgc gcatcgacga
      901 ggaaagccga cacgggaacg gcctgcccat cgtcttctcg actttggtga acggcgagct
      961 gcgcgcggtg gtgcgcaagg cgagcgcgct gttcgtcgat ttcttcgaga ccttcatcga
     1021 ccctctggaa gccgcgctcg gcgtgcaatc gacgcacact atcgggcgtt cgcacagcgc
     1081 gttggacaag ccgggctact cgcatcgcat ggaggcgatc aatttcgcca tgtcgcacga
     1141 tgacggcgcc tcgaaccgcg atctcgacca ggccgacgtc atcctggtgg gcgtctcgcg
     1201 ctgcggcaag acgccgacca gcctgtacct ggcgctgcag ttcggcgtga aggcggcgaa
     1261 ctacccgctg atcccggagg atttcgcgcg catgcagctg ccgcccgccc tggccccgtg
     1321 caaggcgcgc ctgtacggcc tgagcatctc gcccgagcgc ctgcgcgaga tccgccaggc
     1381 gcgccgtccc gacagccgtt acgccgacct ggacaactgc cggtacgagg tcgagcaggc
     1441 cgagcgactg atgcgccgcg agggcatccg ctggatcaac tccaccgcca aatcgatcga
     1501 ggagatcgcc accaacatcc tgcgcgagct caggctcgag cgcccgatgt actgagctgg
     1561 cgcgcgcgct cgtagagcag gatcgcggcg gcggcggcga cgttgagcga ctcgacgtcc
     1621 ggggcgagcg ggatgctgac gcgatgcgac gcctgcgccg cggccgcggg gctcacgccc
     1681 tgcccttcgg cgccgagaat ccagccgatc gatgcggtca ggtccagcgt ggccggcgcg
     1741 tcgccgccgg cggcggccgc gcatacccgc gtgcccggaa attgcgccag cgcgccggcc
     1801 agctcgtcca cgaccgtgat gccgagcgcg aagtggccgc ccatcgcagc acgcaggact
     1861 ttcggcgacc acgggtcggc gcagccgcgc ccgagaacgg ccgtgtccac gccaaaggcc
     1921 gccgcgctgc gcaggatcgc accgacgttg cctgcgtcct ggacgccatc gaggaacacg
     1981 gcatgggtgg cctgcgccac gggcgccgcc ggcgggagct cgatctcggc cgccagcccc
     2041 gctggggtga ccgcgtccgc gacccagcga aacacggggt cgggcagcgt caccggcgcc
     2101 ggaccggcgc gcctcgccag cgccgcgatc tccgcgtttg cctggccgct cgcgctgacc
     2161 agcacggcct tcggccgcgc gccgctgtcc aggtaggccg cgagcaggtg cgcgccctcg
     2221 ataagcgcgc tgcgctcggc acgccggcta cgcccatcgt gcgccagtgc gtgccagcgt
     2281 cgcacgcgcg ggttgtccct cgagcgcagc aggctcacgc gcccagcacc tggcgcacgg
     2341 gggcgaacga gcgccgatgg atctcgcagg cgccgaactg cgccagccgc gcgaggtgct
     2401 ccggcgtcgg gtagcccttg tgctggtcca gcccgtagtg cggaaaacgc tcgtgcagac
     2461 gcagcatttc ggcgtcgcgc gcggtcttgg cgaggatcga ggcggcagag atctgccgca
     2521 cgcggcggtc gccgccgatc accgcacgta ctgcgcagcc aagcaagggg cactggttgc
     2581 cgtcgatcca cgcttcctgc ggcgccaccg cgagcgcttc caccgcgcgg cgcattgcca
     2641 gcagcgatgc ctgcaggatg ttcaggcgat cgatctcctc gacgctcgca ctcgctacgg
     2701 cccaggccaa tgccctgccg cggatcgcct cggcgagtgc ctcgcggcgc ccgggcggca
     2761 gcagcttgga gtccctgagc cccgcgatgc gccggcgcgg atcgagtagc accgcggcgg
     2821 cgtacaccgg gccggcaatt ggtccacggc cggcctcgtc cacgccgcag atcaggcgct
     2881 caggcacggc ccgcatccag cacgccaagg accgcgtcgg ccgccttttg cgcattgccc
     2941 tgcctgagca acgcgtgcat ctcgcggaac cgccgcacct ggcgttcctg cagatcggtg
     3001 tcgcgcagca ggtcgatcaa cgcggccgac agcgccgccg gcgtcgcccg gccctggatc
     3061 agctcgggca cgaggcgctc gccagcgagg atgttcggca tgccgatgta cggcaggtac
     3121 agcatctggc gctgcagcgc ccaggacagg ggcgactggc ggtagacgat gaccatcggc
     3181 gtcttgtgca gcgcggcctc cagtgtcgcg gtgccgctcg ccac
//
LOCUS       DBNHKE010000065        23592 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1069111, whole genome shotgun sequence.
ACCESSION   DBNHKE010000065 DBNHKE010000000
VERSION     DBNHKE010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 23592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23592
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1069111"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC16O9_04225"
     CDS             complement(<1..254)
                     /locus_tag="AC16O9_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6515857.1"
                     /translation="MSLRRCLLLATFLFAPALALPAAPVALIAVEGAIGPATSDFVSR
                     SLETAAKQGAPLVVLRLDTPGGLDTSMRDIIRDILASPVPV"
     gene            complement(282..2141)
                     /locus_tag="AC16O9_04230"
     CDS             complement(282..2141)
                     /locus_tag="AC16O9_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515858.1"
                     /translation="MEVVLTIATVLGGIAAAWYFWDKWVDRKTHPQTAAAIGPVAQTV
                     PRALSGTTVVLHGELRAFGILMGEDVRAARGAVARARQVLVATVGEHGGRLGVAPADA
                     LLAVFDTAAQALACAIGARSALARANAALPPAEHVHYRFGLDLGKPGQADLSPPRATL
                     ERAASIVRRAPTDGIRLTEAVHAQLPDDAGYLLSAVEPGLFALAEGEVPVGSRPVPAQ
                     LESVALPLPDRPSIVLLPFSCTGQDPDTAALADGLRMDIQNALVKMPGVFLIAAGSAN
                     ALRGVDAAEAARRLGVRNALEGAVQRQGERVRVNVQLTDTTAGTVTWSERYDRALDHT
                     FELQDEIAARVVTALDVKLASGEQARIWHKCLTVPKSRDQFYRGMQAFFQMNKESMAA
                     ARACFERVVELAPGSSHGPSWMAMCLWFEVTRGWSSDPERARAAAGEWAERAVGMADA
                     DGQAHTVLGNVRLLQRRFDEALAIAREAAVIRPNCTNANGFLANVLLHCGEPDAALTH
                     IRHAIRYSPVYPPWFVEILAAAYREGGQMDFAVSAAQEGLRIAPQAVNCRLILASALV
                     RGGWAADARRIAGEVAGLAPDFSLARHAAQEPYRDQAVLDRVIGELRSAGLPD"
     gene            2277..7742
                     /gene="uvrA"
                     /locus_tag="AC16O9_04235"
     CDS             2277..7742
                     /gene="uvrA"
                     /locus_tag="AC16O9_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6515859.1"
                     /translation="MPADAIVIRGARQNNLKNLTLTLPAGELIVVTGVSGSGKSSLVF
                     DTVYAEGQRRYVETFSPYARQFLERMDKPQVDAVEGIPPAIAIDQTNPVRTSRSTVGT
                     MTELNDHLKLLYARAAQLHCRGCGKPVRRDTPQSIYAALPQDRVTITFPVPVPKNFSE
                     KEVEALLQRQGYTKLHSRSKTLIEVVQDTVTPGKSEKSRVMDAIESALRFGQGRLVAY
                     AEGIVGSVSNTSADLAGRITRPGRQTLGDPTEALRFSTALHCPDCDLSYSDPTPAQFS
                     FNSPLGACDTCRGFGRVIGVDYGLVIPDESKTLRDGAIRPWQTPSFKECQDDLAKYAK
                     KRGLSLDVPWREMKDADRRWVIDGDDGWVSWKKSWPGKWYGVARFFKWLESKAYKMHI
                     RVLLSKYRAYTECTACNGTRLKPDATLWKLDGKSIHELMLMPVSDVQRLFGGLELPRP
                     LDEATDLLLGEIRTRLAYLGEVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTL
                     FVLDEPSIGLHPRDMGRVIDVMKKLRDAGNSLVVVEHDPQIMLAADRILDMGPGPGER
                     GGEIVFFGDPAELRKKDTLTAQYLSGRKRTEAPRAPRAPAKFVTVRGASEHNLKDIDV
                     AFPLERLVCVTGVSGSGKSTLVQDVLYAALRKAKGKPTETPGRHRALEGAELVGDVVM
                     VDQSPIGKTTRSNPASYVGAFDAIRDLYAKAPVAKERKYTAGTFSFNSGHGRCPACGG
                     NGFEHVEMQFLSDVYLRCPDCNGKRYRPEVLEVALRGKSIADALEMTVSAALVFFEDH
                     AEVLARLRPLADVGLDYLRLGQPVPTLSGGEAQRLKLAGHLTLKAGEERKLFLFDEPT
                     TGLHFEDIAKLLRAFRQLIAAGHSLVVIEHNLDVIRAADWILDLGPEGGAAGGERVCA
                     GTPSEVMRHATSHTAKALRDYEKGTDLISLKSKDREMRSVPNIRIHNAREHNLKSIDV
                     DIPRQRFTVVTGVSGSGKSTLAFDILFSEGQRRYLESLNAYARQFVQTAARPDVDAIY
                     GIPPTVAIEQRTSRGGRKSTVGTLTEIHHFLRLLFVKLGTPHCPDCDVAIEPQSEDAI
                     AARLMKAHRNDTVVLLAPLVVNRKGVYTDLAKWAASKGYSQLRVDGKLLPLRPFPRLD
                     RFKEHTIELPVGYVKVTPRNEKELRERLTRALEHGKGVVHVQTGKHIQVYSTKRACPS
                     CGRSFQELDPRLFSYNSRHGWCPSCVGTGLQLKDVGWDAERSRTGQEDHVLDSWLEWL
                     EVDEACPACEGKRLNPEALAVRWRGRSIADYGMQPVAQLTELFAKVKLEGRSAEIARD
                     LVAELRSRLGFLKEVGLDYLALDRAAPTLSGGEAQRIRLAAQLGSNLRGVCYILDEPT
                     IGLHHRDNRILLDVLEKLEAKGNTLVVVEHDEDTIRRAHHVIDLGPGAGKQGGRVIAE
                     GTADELVQSPESVTGRFLAHPLRHPLQPRRRVAKDAPALEIGGASMHNLKRIDVRVPI
                     ARLSAITGVSGSGKSTLARDVLHDSLKAGGPIGCKTLKGRKLVERVLEVDQAPIGKTP
                     RSCPATYVGFWDAIRRLFADTSEARIRGWGPSRFSFNTKGGRCETCEGQGLKRIEMSF
                     LPDVKVACEACNGLRFNAETLGAHLRGKSVGDVLAMSVDEAVEFFAAHPSINHALRLL
                     QDVGLGYLTLGQQSPTLSGGEAQRIKLVTELAKVRELGGSTHLPSPASSFGRRETAVK
                     GAKTLYVLDEPTVGLHMADVEKLIRVLHRLVDAGNTVVVVEHNLDIIAEADWLIDLGP
                     EGGDAGGRIVYQGPPERASRGHTAQALNAFLAERKAA"
     gene            7739..8629
                     /locus_tag="AC16O9_04240"
     CDS             7739..8629
                     /locus_tag="AC16O9_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6515860.1"
                     /translation="MSALPPPRRAVALALMVVAPVLWSTAGVLSRLIQSAGAFEMAFW
                     RSSIAAVFVFTALSLLQGGRPFATLRAAGLPGLISGAMWAVMFTCFIVALTLTTVANV
                     LVVSSLGPLCTALLTWLMLREPIPTRSWLAIVAASVGMGAMFGAGFTADDWSHVAGLA
                     LALTIPIAGAINIVVLRKSGAHLDLMPAVMLGALISAVVSLPFALPLAASGRDLLVLG
                     TLGVFQLGVPCLMLVLAARTLTAPEVALLALLEVVLGPLWTWLGAGEVPAGATIAGGA
                     IVLAALAGNEAAALAERKRV"
     gene            8882..10099
                     /locus_tag="AC16O9_04245"
     CDS             8882..10099
                     /locus_tag="AC16O9_04245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6515861.1"
                     /translation="MLAGRDLLAAAQTGTGKTAGFTLPILQLLGASQRQIRVLVLVPT
                     RELCAQVQESVRTYGKHSKLRSTVVFGGVGIHPQIAELKRGVDIVVATPGRLLDHVQQ
                     RTIDLRHVEILVLDEADRMLDMGFIHDIRRIMKLLPPKRQNLLFSATFPDEIRQLASN
                     FMHDPVTVEVARRNTPAELVDQVAHPVAHDRKRALLSHLVKTGDWQQVLVFTRTKHGA
                     NRLAEQLERDGIEADAIHGNKSQGARTRALRRFKDQELRVLVATDIAARGLDIESLPH
                     VVNYDLPHVAEDYVHRIGRTGRAGAEGSAVSLVSHEDRPLMAAIERLMNRKVEQRVVP
                     GFEPGAHSPRPQVEAQRSFRPQSRPQHRPQGQQQRHARSSIPHTERLTREQEAQLREA
                     GARLAAGGGMRRR"
     gene            10266..12764
                     /gene="pepN"
                     /locus_tag="AC16O9_04250"
     CDS             10266..12764
                     /gene="pepN"
                     /locus_tag="AC16O9_04250"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR6515862.1"
                     /translation="MREPTPKTIYLKDYTPPAFVVPHVELDVDIREDHALVKAKLQVK
                     RNAGGKGPLVLDGDELELVSVSLDGKPAKHDVAPDRLTVHDVPDAFTLETVTRIVPQK
                     NTKLEGLYATKHGFVTQCEAEGFRRITWFVDRPDNMARYLVTLHADKARYPILLSNGN
                     RTGGGDEAGGRHWAAFDDPFPKPSYLFALVAANLEELKDAFDTRSGKRAELSVYVEPG
                     KLDQAGWAMQCLKRAMRWDEQRFGLELDLAQYKIVAVGDFNSGAMENKGLNIFNTRFV
                     LARPDTATDADYQAIDAVVAHEYFHNWTGDRVTCRDWFQLSLKEGLTVFRDQEYSSDQ
                     YSRAVERIQNVRMLRAGQFPEDAGPQKHPVRPQSYMEIRNFYTMTVYEKGAEVVRMQH
                     TLLGEAKFQAGMKLYFQRHDGHAVTCDDFVQAMQDASGVDLSQFRRWYDVAGTPVLEC
                     AGEHRDGRFTLRVKQSIDPPLHIPLAVKVGPHERVLPVKRAEETFTFDVAEPVVPSLL
                     RGFSAPVILNYPYSESDLVRLMAGDDDPFNRWEAGQRLAADIILKRGGEPSAAFLEAA
                     RRVLAEKDHAFVAEALALPSEAFLAEQMAVVDPDALHEARNTLRRRIAEALEPELAAA
                     YRALESRAPYAPEPAAIGRRALRNLCLGYLVELGRSALADAQFRRADNMTDAMAALAC
                     LANADCPEREPALQAFYDKWHDEPLVVDKWLSVQALSRLPGTLARVRGLLAHPAFDIK
                     VPNKVYALVRAFAANHVRFHAADGAGYAFLAERVLELDRLNPQVAARMARGFDRWRKF
                     DAARQALARAQLERIRDAAGLSRDVAEIVGKSLA"
     gene            12775..13998
                     /locus_tag="AC16O9_04255"
     CDS             12775..13998
                     /locus_tag="AC16O9_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HIR6515863.1"
                     /translation="MATTVLAVFAVVYLGMLLGGLPFLQLDRTGVALLGAIALVALGA
                     VTPAEAAGAVHLPTLLLLFSFMVISAQMRLGGFYTRVTLDLADLRVSPAALLGLVIGV
                     VGALSAVFSNDIICLAIAPVLADACLARRLDPVPYLLGLACAANVGSAATLIGNPQNM
                     LIGQTLALSFSGYLVEAIAPVLLGLAATWGILVWQTRGRWRTVLDDPPPERREDYAVY
                     DRWQTAKGLGVALALVGAFLLSDVPREVAALAGAGLLLMSRRFHSHKMLGLVDWELLV
                     LFIGLFVVNHALAQTGIAAAALAWLSDAGTPLTDTGPLFLATLVLSNLVSNVPAVMLL
                     LPAATEPFSGPMLALVSTLAGNLLIVGSIANIIVVDAARRRGIAIDWRTHARTGVPVA
                     LVTLCVTAVWFALRH"
     gene            14125..14838
                     /locus_tag="AC16O9_04260"
     CDS             14125..14838
                     /locus_tag="AC16O9_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HIR6515864.1"
                     /translation="MTSPDITSRLSTTGQLLVRSGIEIGRLLDAMAQDRSAVTAQVPP
                     QLLFLSQLLYVEPVRGYMMLSYSDHKPANSAALSARSLTFRCNHRGAQFAFTGDGPRQ
                     SAHKGQPCIQCALPSAVLGMQRRGAGRVQVPAQAPVSCDLRMGLISFPGRVVDVSLDG
                     IGTLVTDPAIPLCAGTRLERVRISHPQCTPFLIDFEIRYVTRVVLANGEHASRIGCQV
                     VGARERLEELIRLFIIDLA"
     gene            14848..15543
                     /locus_tag="AC16O9_04265"
     CDS             14848..15543
                     /locus_tag="AC16O9_04265"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR6515865.1"
                     /translation="MPENPGTVDSTLYLILATLLFLGIHALPATRLRAAAVRALGEGA
                     YQGLFSLASLATLVWMSLEYERAPFEGLWPGLRLVPIAVLPVAFVLVACGALARNPTA
                     AGQAGALRQEDPARGIIRVTRHPVMWAIMLWAGAHLLAIGSLQAVIFFGGLLLLAAAG
                     TTLQDARKAAQLGEDWKRFAALTSNLPFMAIAQGRNRVVWREIGWWRPAVGLVAFALL
                     LGLHAWLFGVRPY"
     gene            complement(15601..16497)
                     /gene="ltnD"
                     /locus_tag="AC16O9_04270"
     CDS             complement(15601..16497)
                     /gene="ltnD"
                     /locus_tag="AC16O9_04270"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HIR6515866.1"
                     /translation="MMTAVGVIGLGSMGGGVAKSLLAKKFETHVYDVRADVVKAFVAQ
                     GAKAAATPAALGAACEVVVILVVNAEQTEKVLFGEQGAAAAMRKGAVVVSSATLAPDV
                     AEQFGARLEQMGLLMIDAPVSGGAAKAAAGEMTIMASGAPEAFARCEPVLAAIAAKVY
                     RLGDRPGPGSKVKMINQLLAGVHIAAACEAMALGMRAGADPKTLYEVISNSAGSSWMF
                     QNRVPHILAGDYTPLSAVNIFVKDLGIVLDTAKKFGFPLPLTATAHQLYLAAAAQGHG
                     LEDDAAVVKVYAALASLDLPAK"
     gene            complement(16494..16895)
                     /locus_tag="AC16O9_04275"
     CDS             complement(16494..16895)
                     /locus_tag="AC16O9_04275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6515867.1"
                     /translation="MDLLKPGLRGRAELMVAEEHTAPRVGSGRVRVLATPVMINLFEA
                     AALDAVERLLPPGRQSLGTVLNVRHIAATPVGMRVTATAEVTGVEGRTIRFRLEARDE
                     RDLIGDGSHERVVVNVAKFDERVQRKLKGEG"
     gene            16916..18166
                     /locus_tag="AC16O9_04280"
     CDS             16916..18166
                     /locus_tag="AC16O9_04280"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6515868.1"
                     /translation="MTPRVVILGCGFGGLFAARALRKAPVEVTVVDRTNHHLFQPLLY
                     QVATAGLAAPAIAEPIRRALAMQRNVTVLYGEAQRVDVAAHAVVLENGDTLPYDRLIL
                     ATGATDSYFGHDEWRAHAPGLKTLEDAFEIRSRILLAFEHAEREADPVKRAAWLTFVV
                     IGAGATGVEMAGMVAEISRHTLKGEFRRFDPRNARVVLVEGADRVLPSYTPDLSERAR
                     VQLERLGVTVWLGRKVTDIDARGVQLGGDRLEAMTVVWCAGVAASPIGATLGVPLAHG
                     GRVIVEPDLSVPGHPEIQVVGDLAFLPEHEPPVPGVAPAAKQMGRQAAHNIVAALASR
                     PAQAFRYRDYGQLATIGRSKAVAMFGRIHVWGWLAWVAWLTAHIYFLIGFRNRLVVLI
                     DWAWAYWTFERSARIVDKGARPPS"
     gene            complement(18163..19050)
                     /locus_tag="AC16O9_04285"
     CDS             complement(18163..19050)
                     /locus_tag="AC16O9_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6515869.1"
                     /translation="MSGGRRIGIDLGGTKIEALVLDDEGREVFRKRVPAPRAAYEATL
                     AAVAALVREAGAGTIGIGIPGALSPASGLVKNANSTQLIGHPLKQDLEHALGREVRIA
                     NDANCFALSEAVDGAGAGAEVVFGVILGTGVGGGVVVRGEVLTGPNAIAGEWGHNPLP
                     LPVAGDLPLPACYCGRAGCIETYLSGPALARDHEIATGERVAPEEIVRRGADATLARY
                     EERLARALGSVINLIDPDVVVLGGGLSNVRRLYDNVPRIWGRYIFSDLVATRLLPPVH
                     GDASGVRGAAWLWGGPPRG"
     gene            19211..20803
                     /locus_tag="AC16O9_04290"
     CDS             19211..20803
                     /locus_tag="AC16O9_04290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6515870.1"
                     /translation="MAQASIAADYVVVGAGSAGCLLANRLSRDPAVRVVLLEAGGRDD
                     YFWIDIPVGYLYTIANPRTDWCYQTSADEHLDSRSIHYARGRGLGGCSSINAMIHMRG
                     QKADYDHWAALGNRGWSWDEVLPVFQSVEDYQHGAVDTYGAGGELRVEDPRVRWEILD
                     AWRAAAAECGIAPIKVFNTGDNTGCAYFQMNQKRGVRWSATKAFLRPVLHRKNLTVLT
                     GAHVTGLQFEGRACTGVTFRAGRDARSATASREVILAAGAIGSPQILQLSGVGPGALL
                     QQHGIPVVHDLPGVGENLHDHLQIRMQYRVSGVKTLNTMANSLWGKAAMALEYLAFRT
                     GPLTMPPSQLGAFSKSDPAQPTANIEWHVQPLSLDKFGEPLHRFPAITPSVCNLRPAS
                     RGWVRIRANDPFTYPEIKLNYLSAPEDRQVAADAMRATRRIMAARALAKYRPEEFKPG
                     AAAQTQEALEQAAGELGTTIFHPVGTCAMGSVVDERLRVRGIGRLRVIDAAVMPRITS
                     GNTNAPTTMIAEKGARMVLADA"
     gene            complement(20800..21591)
                     /locus_tag="AC16O9_04295"
     CDS             complement(20800..21591)
                     /locus_tag="AC16O9_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR6515871.1"
                     /translation="MLNPIEQHSHARSDDAPALLVAALHAACAAHYEPAGHVFDYARF
                     AESPEYAELGVAARALADFDCASLHIGLRLAFWLNVYNALVLHAVVARRALAGVRALG
                     DFFSGSQYMVGGHVFSLDEIEHGLLRVNAPRLAFGATPLRRDGPRYALAPYMFDERVH
                     FAMYSACRSSPAPAAYAAEDLTGDLERATCAYLASHVRLAAGGATLEVPKLFDWYAVD
                     FGGRRGVLEFILAHVQDDGVAAAAERHGLGLRLRYTEFDWTLNAG"
     gene            complement(21618..22166)
                     /locus_tag="AC16O9_04300"
     CDS             complement(21618..22166)
                     /locus_tag="AC16O9_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515872.1"
                     /translation="MVAQAGGRGHGFQALAALALLLAACAAPPPLDVPAAVAKTYHSM
                     PQASIPLPPASGAATLAEYQREIAGLLHAANAGRVFEGPPPNPLRAVIVMRAEIDVLG
                     EARRLDLVRAPWHNPWLPELVAQTLRQAQPFPRPSMKLLDGGSSVTFTETWLFDYEGR
                     FRLRSLSQAQAEPQHDDEELVP"
     gene            22206..23090
                     /gene="htpX"
                     /locus_tag="AC16O9_04305"
     CDS             22206..23090
                     /gene="htpX"
                     /locus_tag="AC16O9_04305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HIR6515873.1"
                     /translation="MKRVVLFLATNIAVLLLLSVVISVLGLDRWLAAEGINYQGLLVF
                     SAVIGFGGAFISLAMSKTMAKWSTGAHVIDGTEGTTEYWLVQTVKRLAEKAGIGMPEV
                     AVYEGAPNAFATGAFRNSSLVAVSTGLVQSMSKEEVEAVLGHEIGHVANGDMVTLTLI
                     QGVVNTFVIFLSRVVAFFVDKVIFRTERGVGPGFYITAIVFQIVFGVLASIIVAWFSR
                     QREFRADAASADLLGNPRPMMNALARLGGLEPGALPQSMKAAGITDKPSGFAALFSTH
                     PPVEERIAALQARSAGRA"
     gene            23125..>23592
                     /locus_tag="AC16O9_04310"
     CDS             23125..>23592
                     /locus_tag="AC16O9_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR6515874.1"
                     /translation="MKIGIVGVSGYGGSELLRLAAGHPAFELAYVGGESTAGQTLAQR
                     FPALAGHAAGKLVIQPFVPETISGIELLFASLPTGKSREPLARVAKDIRIVDIGGDHR
                     FVEGWTYGLTELPGQRDKIRGAHRLANPGCYPAASLLALAPLVREGLIEPEGIV"
BASE COUNT         3581 a         8500 c         7988 g         3523 t
ORIGIN      
        1 accggcaccg gcgaagcgag gatgtcgcgg atgatgtcgc gcatcgacgt gtccaggccg
       61 ccgggcgtat cgaggcgcag caccaccagc ggcgcgccct gcttggcggc cgtctcgagg
      121 ctgcggctga cgaagtcgga cgtcgccggg ccgattgcac cctcgacggc gatcagcgcc
      181 acgggcgcgg caggcagcgc gagcgccggc gcaaagagga aggtcgcgag cagcaggcaa
      241 cgccgcagac tcatgtgcag actataaacc tatattcgcg gtcagtccgg gaggcctgca
      301 gaccgtaatt cgccaatgac gcggtcgagc accgcttgat cgcgataggg ctcctgcgcc
      361 gcgtgccgcg caagcgagaa gtccggcgcg agacccgcga cctcgcccgc aatgcgccgc
      421 gcatctgctg cccagccgcc gcgcaccagc gcgctcgcga ggatcagccg acagttgacc
      481 gcctgcgggg cgatccgcag cccctcctgc gcggcgctga ctgcgaaatc catctgaccg
      541 ccctcccggt aggcggcggc gagaatttcg acaaaccaag gtggatagac tggggaatag
      601 cgaatcgcat gcctgatgtg cgtcaaggcc gcatccggct cgccgcagtg caggagcacg
      661 ttggcgagaa aaccgttcgc gttggtgcag ttcggccgta tgacggcagc ctcgcgcgcg
      721 atggcaagcg cctcgtcgaa cctgcgctgc agtagccgga cgtttccgag caccgtgtgc
      781 gcctggccgt cggcgtctgc catgccgacc gcccgctccg cccactcgcc ggcggcagcg
      841 cgcgcccgct ccggatcact gctccagcct cgcgtcacct cgaaccacag gcacatcgcc
      901 atccacgagg gcccgtgcga cgaaccaggc gccagctcga ccacccgctc gaaacaggcg
      961 cgcgcggccg ccatcgattc cttgttcatc tggaagaagg cctgcatgcc gcgatagaac
     1021 tgatcgcgtg atttcggcac agtcaggcat ttgtgccaga tccgcgcctg ctcgccgctc
     1081 gccagcttca cgtcaagtgc ggtaaccact cgcgccgcga tctcgtcctg gagttcgaac
     1141 gtgtgatcga gcgcccggtc gtaccgctcg gaccatgtca ccgtgccggc agtcgtgtcg
     1201 gtgagttgca cgttcacccg cacgcgttcg ccttggcgct gcacagcacc ttcgagcgca
     1261 ttacgcacgc ccagacgccg cgcagcttcg gcggcatcga cgccgcgcag ggcattggcg
     1321 gaccccgccg cgatcaggaa cacgcccggc atcttgacga gcgcgttctg aatgtccatg
     1381 cgcagtccgt cggcgagcgc ggcggtgtcg gggtcctggc cggtgcagga aaacggcagc
     1441 agcacgatcg atggccgatc ggggagcggc agcgcgacgg actcgagttg cgccggaacg
     1501 ggccgtgacc cgaccggcac ctcgccttcg gctagcgcga acaacccagg ctccactgcc
     1561 gacagaagat atcccgcgtc gtccggaagc tgggcatgca ccgcctccgt caagcgaatc
     1621 ccgtctgtcg gggcgcgccg cacgatcgat gccgcgcgct cgagcgttgc cctggggggc
     1681 gacaggtcgg cctggccggg tttccccaaa tcgagcccga accggtaatg cacgtgctct
     1741 gccggaggca gcgccgcgtt ggcgcgcgca agcgcgctgc gcgcgccaat tgcgcaggcc
     1801 agcgcttgcg ccgcggtatc gaacaccgcg aggagcgcgt cggccggcgc caccccaagc
     1861 cggccgccat gctcgccgac agtggcgacg agcacctgtc gcgcgcgcgc gaccgcgcca
     1921 cgtgccgctc gcacgtcctc tcccatgagg atgccaaatg cacgcaattc tccgtgcaga
     1981 acgacagtgg tgccggaaag cgcgcgcggc accgtctgcg ccaccgggcc aatcgctgca
     2041 gccgtttgcg ggtgcgtctt acggtcgacc cacttatccc agaaatacca ggcggccgcg
     2101 atgccgccga ggaccgttgc tatggtaagc accacctcca tccgtattcg ccccctgttt
     2161 gcacgtcgcc ttcgattgcg aggccaattc aatcagaagc gggcgcagat cgccatacgc
     2221 tgtcggctag aatgcggtct ttccgccggc aaatccccct cttgaagcag gaacagattc
     2281 ccgccgacgc catcgtcatt cgcggcgcgc gccagaacaa tctcaagaac ctgacgctga
     2341 cgctgcccgc gggcgagctg atcgtggtga ccggcgtttc gggctcgggc aagtcctccc
     2401 tcgtcttcga caccgtctac gccgagggcc agcgccgcta cgtcgagacc ttcagcccct
     2461 acgcgcgcca gttcctagag cgcatggaca agccgcaggt ggatgccgtg gagggcatcc
     2521 cgccggccat cgccatcgac cagaccaacc cggtgcgcac ctcgcgctcc acggtcggca
     2581 ccatgaccga gctgaacgac caccttaagc tgctctacgc gcgcgcggcc cagctgcact
     2641 gccgcggctg cggcaagccg gtgcgccgcg atacgccgca gtcgatctac gccgcgctgc
     2701 cgcaggacag ggtgacgatc acgttcccgg tgccggtgcc gaagaacttc tctgaaaagg
     2761 aagtggaagc gctcctgcag cgccagggct acaccaagct gcactcacgc tccaagaccc
     2821 tgatcgaagt agtgcaggac accgtgaccc cggggaagtc cgagaagtcg cgggtaatgg
     2881 acgccatcga atctgccctc cggtttgggc aagggcgttt ggttgcctac gctgaaggta
     2941 tcgtcggttc ggtttcaaat acatctgccg acttggccgg aagaatcacc cggccaggtc
     3001 ggcagaccct aggagaccca acggaggcac tgcggttttc tacggctctg cattgcccgg
     3061 actgcgatct cagctattcc gaccccaccc cggcgcagtt ctccttcaac tccccgctcg
     3121 gcgcctgcga cacctgccgc ggcttcgggc gcgtgatcgg cgtcgactac gggctggtga
     3181 ttccggacga gtcgaagacg ctgcgcgacg gcgcgatccg gccctggcag acgccgagct
     3241 tcaaggaatg ccaggacgac ctggcgaagt acgcgaagaa gcgcggcctg tcgctcgacg
     3301 tcccgtggcg cgagatgaag gacgccgacc gccgctgggt gatcgacggc gacgacggct
     3361 gggtgagctg gaagaagtcc tggcccggca agtggtacgg cgtggcgcga ttcttcaagt
     3421 ggctggagtc gaaggcctac aagatgcaca tccgcgtgct gctctccaag tatcgcgcgt
     3481 acacggagtg cacggcctgc aacggcacgc gcctgaagcc cgacgcgacg ttgtggaagc
     3541 tggacggcaa gtcgatccac gagctgatgc tgatgccggt gagcgacgtg cagcgcctgt
     3601 tcggcggcct cgagctgccc aggccgctcg acgaggccac cgaccttctg ctcggcgaga
     3661 tccgcacgcg cctggcctac ctgggcgaag tgggtctggg ctacctcacg ctcgaccggc
     3721 agtcgcgcac cctttccggc ggcgaggtgc agcggatcaa cctgaccacg gcgctcggca
     3781 cttcgctggt gaatacgctc ttcgtgctcg acgagccttc catcggcctg cacccgcgcg
     3841 acatggggcg cgtgatcgac gtgatgaaga agctgcgcga cgcgggcaac tcgctcgtcg
     3901 tcgtcgagca cgacccgcag atcatgctgg ccgccgaccg catcctcgac atgggcccgg
     3961 gccccggcga gcgcggcggc gagatcgtgt tcttcgggga tccggccgaa ctgcgcaaga
     4021 aggacacgct caccgcgcag tacctgtcgg ggcgcaagcg caccgaggcg ccgcgcgccc
     4081 cgcgcgcgcc ggcgaagttc gtcacggtgc gcggcgcgtc cgagcacaac ctcaaggata
     4141 tcgacgtcgc tttcccgctg gagcgcctgg tgtgcgtgac gggcgtctcc ggatcgggca
     4201 agtccacgct ggtgcaggac gtgctctacg ccgcgctgcg caaggcgaag ggcaagccga
     4261 ccgagacgcc cggaaggcac cgcgcgcttg aaggcgccga gctcgtgggc gacgtcgtca
     4321 tggtggatca gtcgccgatc ggcaagacca cgcgctccaa tccggcaagc tacgtcggcg
     4381 ccttcgacgc cattcgcgac ctgtacgcca aggcgccagt ggcgaaagag cgcaagtaca
     4441 ccgcgggtac cttcagcttc aactcgggcc acggccgctg cccggcctgt ggcggcaacg
     4501 gcttcgagca cgtcgagatg cagttcctct ccgacgtcta cctgcgctgc ccggactgca
     4561 acggcaagcg ctaccggccc gaggtgctcg aggtggcgct gcgcggcaag agcatcgccg
     4621 atgcgctgga gatgacggtg tctgcggccc tcgtgttctt cgaggaccac gccgaggtcc
     4681 tcgcgcggct gcggcccctg gccgacgtcg gcctggatta cctgcgcctc ggccagccgg
     4741 tgccgaccct ttcaggcggc gaagcgcagc gcctcaagct tgccgggcac ctgacgctga
     4801 aggccggaga ggagcgcaag ctcttcctgt tcgacgagcc gaccaccggc ctgcacttcg
     4861 aggacatcgc gaagctgctg cgcgcgttcc gccagctcat cgcggccggg cactcgctgg
     4921 tcgtcatcga gcacaacctc gacgtgatcc gcgccgccga ctggatcctc gacctcggtc
     4981 ccgagggcgg cgccgcgggc ggcgagcggg tgtgcgcagg aacgccttcg gaggtgatgc
     5041 ggcacgcgac gtcgcacacc gcgaaagcgc tgcgcgacta tgaaaagggg acagacctca
     5101 tttccttgaa atcaaaggac agggaaatga ggtctgtccc caatatacgc atccacaacg
     5161 cacgcgagca caacctcaag tcgatcgacg tcgacatccc gcgccagcgc ttcacggtgg
     5221 tgaccggggt gtcgggctcg ggcaagtcca cgctcgcctt cgacatcctg ttctccgagg
     5281 gccagcgccg ctacctcgag tcgctgaacg cctatgcgcg ccagttcgtg cagaccgccg
     5341 cgcggcccga tgtcgacgcc atctacggca tcccgccgac cgtcgccatc gagcagcgca
     5401 ccagccgcgg cggccgcaag agcaccgtcg gcacgctcac cgagatccac catttcctgc
     5461 ggctcctttt cgtcaagctc ggcacgccgc actgccccga ctgcgacgtg gcgatcgaac
     5521 cgcagagcga ggacgcgatc gccgcgcgcc tgatgaaggc gcaccgcaac gacaccgtgg
     5581 tgttgctcgc gccgctcgtc gtcaaccgca agggcgtcta caccgacctc gcgaagtggg
     5641 ccgcgtccaa gggctactcg cagctgcgcg tggacggcaa gctcctgccg ctgcggccct
     5701 ttccgcgcct cgaccgcttc aaggagcaca ccatcgagct gccggtcggc tacgtgaagg
     5761 tgacgccgag gaacgagaag gagctgcgcg agcggctcac gcgcgccctc gagcacggca
     5821 agggcgtggt gcatgtgcag accgggaagc acatccaggt ctactccacc aagcgcgcct
     5881 gcccctcgtg cgggcgcagc ttccaggagc tcgacccgcg cctcttttcg tacaactcgc
     5941 gccacggctg gtgcccgagc tgcgtcggca ccgggttgca gctgaaggac gtgggctggg
     6001 acgcggagcg ctcgcgcacg gggcaggaag accacgtgct cgactcgtgg ctcgagtggc
     6061 tggaagtgga cgaagcctgc cccgcctgcg agggcaagcg cctcaatccc gaggcgctcg
     6121 ccgtgcgctg gcgcggccgg agcatcgccg actacggcat gcagccggtg gcgcagctca
     6181 ccgagctgtt cgcgaaggtg aagctggagg gccgcagcgc cgagatcgcg cgcgacctcg
     6241 tcgccgagct gaggagccgc ctcggcttcc tgaaggaagt cggcctcgac tatctcgcgc
     6301 tcgaccgcgc cgcgcccacg ctctcgggcg gcgaggcgca gcggatccgg ctggccgccc
     6361 agctcggctc caacctgcgc ggcgtgtgct acatcctcga cgaaccgacc atcggcctgc
     6421 accaccgcga caaccggatc ctcctcgatg tgctggaaaa gctcgaggcc aagggcaaca
     6481 ccctggtggt cgtggagcac gacgaggaca ccatccgccg cgcgcaccac gtcatcgacc
     6541 tcggcccggg cgcgggcaag caggggggcc gcgtcatcgc cgaaggcacc gccgacgaac
     6601 tggtgcagtc tcccgagtcc gtcaccgggc gcttcctcgc ccaccccttg cgccacccgc
     6661 tgcagccgcg ccgccgcgtg gcgaaggacg cgccggcgct cgagatcggc ggcgcctcga
     6721 tgcacaacct gaagcgcatc gacgtgcgcg taccgatcgc gcgcctcagt gccatcaccg
     6781 gcgtctccgg ctcgggcaag tcgacgctcg cgcgcgacgt gctgcatgac agcctgaagg
     6841 cgggcggccc catcggctgc aagaccctga agggccgcaa gctcgtcgag cgcgtgctcg
     6901 aagtcgacca ggcgccgatc ggcaagaccc cgcgctcctg ccccgcaacg tacgtgggct
     6961 tctgggacgc gatccggcgc ctgttcgccg acacgagcga agcgcgcatc cgcggctggg
     7021 gcccgagccg cttttccttc aacaccaagg gcgggcgctg cgagacctgc gagggccagg
     7081 gcctgaagag aatcgagatg agcttcctgc ccgacgtgaa ggtggcatgc gaagcctgca
     7141 acggcctgcg cttcaacgcc gaaacgctcg gcgcgcacct gcgcggcaaa tcggtgggcg
     7201 acgtcctggc gatgagcgtc gacgaggcgg tggagttctt cgccgcgcat ccgtcgatca
     7261 atcatgcgct gaggttgctg caggacgtgg ggctcggcta cctcacgctc gggcagcaga
     7321 gcccgacgct gtcaggagga gaggcgcaga ggatcaagct tgtcacggaa ttggcgaagg
     7381 taagggagct cgggggttct acacatctcc cgtctcccgc ttcttccttt gggagacggg
     7441 agacagcggt taaaggcgca aaaacactct acgtcctcga cgaacccacc gtaggcctgc
     7501 acatggccga cgtcgaaaag ctgatccgcg tgctgcaccg cctggtcgac gccggcaaca
     7561 cggtggtcgt ggtcgagcac aatcttgaca tcatcgccga agccgactgg ctgatcgacc
     7621 tcggccccga aggcggcgac gccggcggcc ggatcgtgta ccagggtccg cccgagcgcg
     7681 cctcgcgcgg ccataccgcg caggcactga acgcgttcct cgcggagcgc aaggccgcgt
     7741 gagcgcgctt cccccgccgc ggcgcgcggt cgcgctggcg ctgatggtcg tggcgccggt
     7801 gctgtggagc accgcggggg tgctttcgcg cctgatccag tccgcgggcg ccttcgagat
     7861 ggcgttctgg cgctcgagca tcgccgccgt gttcgtgttc accgcgctct cgctgctgca
     7921 gggcgggcgg cccttcgcca ccctgcgcgc ggcgggcctc cccggcctga tctccggcgc
     7981 catgtgggcg gtgatgttca cctgcttcat cgtcgcgctc acgctcacca ccgtcgccaa
     8041 cgtcctggtg gtgtcgagcc tgggcccgct ttgcaccgcg ctgctcacct ggctcatgct
     8101 gcgcgagccg attcccacgc gcagctggct cgcgatcgtc gccgcgagcg tcggcatggg
     8161 cgccatgttc ggcgcggggt tcacggccga cgactggagc cacgtcgccg ggctcgcgct
     8221 cgcgctcacc atcccgatcg cgggcgcgat caacatcgtg gtgctgcgaa agagcggtgc
     8281 gcacctggat ctgatgcccg ccgtgatgct cggcgcgctg atctcggcgg tggtgtcgct
     8341 gcccttcgcg ctgccgttgg ccgcgagcgg gcgcgacctg ctggtgctcg gcacactcgg
     8401 cgtgttccag ctcggcgtgc cctgcctgat gctggtgctc gccgcccgca cgctcaccgc
     8461 gcccgaagtc gcgctgctcg cgctgctgga ggtggtgctg gggccgctgt ggacctggct
     8521 cggtgcgggc gaagtgcccg cgggcgcgac catcgccggc ggcgccatcg tgctcgcggc
     8581 gctggcggga aacgaggccg ccgcgctcgc cgagcgcaag cgtgtataat gcgctccagg
     8641 tttctgcagc gcgtacttcc ggtttgcgtt tcagtcgtta gtcagtccat cctggacttc
     8701 gaataacaaa gcctaagagc gtcggcctga accggtagaa gggttctcct tcccgggccg
     8761 gcaccaggag aaagacatta cttcgttcga ttcgctcggg ctcaagcccg agctgctccg
     8821 cgctgtcgcg gaccagggct acaccacgcc cacccccatc caggcgcaag ccatccccgt
     8881 cgtgctcgcc ggccgcgacc tgctcgccgc cgcgcagacc ggcaccggca agactgccgg
     8941 gttcaccctg ccgatcctgc agctgctcgg cgcctcgcag cgccagatcc gcgtgctcgt
     9001 gctggtgccg acgcgcgagc tgtgcgccca ggtccaggaa agcgtgcgca cctacggcaa
     9061 gcactcgaag ctgcgctcga ccgtcgtctt cggcggcgtc ggcattcacc cgcagatcgc
     9121 cgagctcaag cgcggcgtcg acatcgtcgt cgccacgccg gggcgcctcc tcgaccacgt
     9181 gcagcagcgc acgatcgacc tgcgccatgt cgagatcctg gtcctcgacg aggccgaccg
     9241 catgctcgac atgggcttca tccacgacat ccgccgcatc atgaagctgc tgccgcccaa
     9301 gcgccagaac ctgctgttct cggccacctt ccccgacgag atccgccagc tcgccagcaa
     9361 cttcatgcat gacccggtca ccgtggaagt cgcgcggcgc aacacccccg ccgagctcgt
     9421 cgaccaggtc gcgcacccgg tggcgcacga tcgcaagcgc gcgctgctgt cgcacctggt
     9481 gaagaccggc gactggcagc aggtgctggt cttcacgcgc accaagcacg gcgcgaaccg
     9541 gctcgccgag cagctcgagc gcgacggcat cgaggccgac gccatccacg gcaacaagag
     9601 ccagggcgcg cgcacccggg cgctcaggcg cttcaaggac caggagctgc gcgtgctggt
     9661 ggcgaccgac atcgccgcgc gcggcctgga catcgagtcg ctgccgcacg tcgtcaacta
     9721 cgacctgccg cacgtcgccg aggactacgt gcaccgcatc ggccgcaccg gccgcgcggg
     9781 ggccgagggc tcggcggtgt cgctggtgtc gcacgaggac cggccgctga tggcggcgat
     9841 cgagcggctc atgaaccgca aggtcgagca gcgcgtggtg ccggggttcg agccgggagc
     9901 gcattcgccg cggccgcagg tcgaggcgca gcggtccttc cggccgcagt cgcgtccgca
     9961 gcatcgtccg cagggacagc agcaaagaca tgcgcgctcg agcatcccgc atacggagcg
    10021 cctgacccgc gagcaggaag cgcagctgcg ggaagccggc gcgcggctgg ctgccggcgg
    10081 tggtatgcgg cggcggtagc ggtcaggacg gcaatcttcc gcgccggccg cgcagcttcc
    10141 gcagttcggc tgcagcggtg ccggtggcgg caagcgccgc cgccccggcg gtacccacgc
    10201 gatagggcgt acgcagcacg gggcatgcct tgacctggca cacgctcggt taaactcggg
    10261 cgaccatgcg cgagcccacc ccgaagacca tctacctcaa ggactacacg ccgcccgcct
    10321 ttgtcgtgcc gcacgtggag ctcgacgtcg acatccgcga ggaccacgcc ctcgtcaagg
    10381 cgaagctgca ggtgaagcgc aacgcgggag gcaaggggcc gctggtgctg gacggcgacg
    10441 agctcgagct cgtgtcggtg tcgctggacg gcaagcccgc gaagcacgac gtcgcccccg
    10501 accgcctcac ggtgcacgac gtgcccgacg ccttcacgct cgaaaccgtg acgcgcatcg
    10561 tgccgcagaa gaacaccaag ctcgagggcc tgtacgccac gaagcacggc ttcgtcacgc
    10621 agtgcgaggc ggaaggcttt cgccgcatca cctggttcgt cgaccgcccc gacaacatgg
    10681 cgcgctacct cgtcacgctg cacgccgaca aggcgcgcta cccgattctg ctctccaacg
    10741 gcaaccgcac cggcggcggc gacgaggccg gcgggcggca ctgggcggcg ttcgacgacc
    10801 cgttccccaa gccctcgtac ctgttcgcgc tggtggcggc gaacctggag gagctgaagg
    10861 acgcgttcga cacccgctcg ggcaagcgcg ccgagctttc cgtctacgtt gagcccggca
    10921 agctcgacca ggcgggctgg gcgatgcagt gcctgaagcg cgccatgcgg tgggacgagc
    10981 agcgcttcgg cctcgagctc gacctcgcgc agtacaagat cgtggcggtg ggcgacttca
    11041 actccggcgc catggagaac aagggcctca acatcttcaa cacgcgcttc gtgctggcgc
    11101 gccccgacac cgccaccgat gccgactacc aggcgatcga cgcggtggtg gcgcacgagt
    11161 acttccacaa ctggacgggc gaccgcgtca cctgccgcga ctggttccag ctgtcgctga
    11221 aggaaggcct cacggtgttc cgcgaccagg agtacagctc cgaccagtac tcgcgcgccg
    11281 tcgagcgcat ccagaacgtg aggatgctgc gcgccgggca gttccccgag gacgcgggac
    11341 cgcagaagca cccggtgcgc ccgcagtcct acatggagat ccgcaacttc tacaccatga
    11401 ccgtgtacga gaagggcgcg gaggtggtgc gcatgcagca cacgctgctg ggcgaagcga
    11461 agttccaggc cgggatgaag ctgtacttcc agcgccacga cggccacgcg gtcacctgcg
    11521 acgatttcgt gcaggcgatg caggacgcct cgggcgtcga cctgtcgcag ttccggcgct
    11581 ggtacgacgt cgccggcacg ccggtgctcg agtgcgccgg tgagcaccgc gacggccgct
    11641 tcaccctgcg cgtgaagcag tcgatagacc cgccgttgca catcccgctg gcggtgaagg
    11701 tgggtccgca cgagcgcgtc ctgccggtga aacgcgccga ggagaccttt accttcgacg
    11761 tggcggagcc tgtggtgccg tcgctgctgc gcggcttttc cgctccggtg atcctgaact
    11821 acccgtacag cgaatccgac ctggtgcgcc tgatggccgg ggacgacgat cccttcaacc
    11881 gctgggaagc gggccagcgg ctcgccgccg acatcatcct caagcgcggc ggcgagccct
    11941 cggccgcgtt cctcgaggcc gcgcgccgcg tcctggcgga aaaggaccac gcgttcgtcg
    12001 ccgaggcgct cgccctgccc tcggaagcgt tcctcgccga gcagatggcg gtcgtcgatc
    12061 cggatgcgct gcacgaggcg cgcaacaccc tgcgccggcg gatcgccgaa gcgctggagc
    12121 cggagctggc cgcggcctac cgcgcactgg agtcgcgcgc gccgtacgcg cccgagcccg
    12181 cggcgatcgg ccggcgcgcg ctgcgcaacc tctgcctcgg ctacctcgtg gagctgggcc
    12241 ggagcgcgct cgccgacgcg cagttccgcc gcgccgacaa catgaccgac gccatggcgg
    12301 cgctcgcctg cctggccaac gccgactgtc ccgagcgcga gccggcgctg caggcgttct
    12361 atgacaagtg gcacgacgag ccgctggtgg tcgacaagtg gctctccgtg caggccctgt
    12421 cgcgcctgcc cgggacgctc gcgcgcgtgc gcgggctgct cgcgcatccg gcgttcgaca
    12481 tcaaggtgcc gaacaaggtg tacgcgctgg tgcgcgcttt tgccgccaac cacgtgcgct
    12541 tccacgccgc cgacggtgcg ggctatgcgt tcctcgccga gcgcgtgctc gagctcgacc
    12601 gcctcaatcc gcaggtggcg gcgcgcatgg cgcgaggctt cgatcgctgg cgcaagttcg
    12661 acgccgcgcg ccaggcgctc gcccgcgcgc agctcgagcg catccgcgac gcggccgggc
    12721 tgtcgcgcga cgtggccgag atcgtcggca agtcgcttgc ctgagggcgc ggccatggcg
    12781 acgaccgtcc tcgccgtgtt cgccgtcgtc tacctgggaa tgctgctcgg cgggctgccg
    12841 ttcctgcagc tcgatcgcac gggcgtggcg ctgctcggcg cgatcgcgct ggtcgccctc
    12901 ggcgcggtga cgccggcgga ggcggcgggc gcggtgcacc tgcccacgct gctgctgctt
    12961 ttctcgttca tggtgatctc ggcgcagatg cgcctgggcg gcttctacac gagggtgacg
    13021 ctcgacctcg ccgacctgcg cgtctcgccg gcggcgctgc tcggtctggt gatcggcgtg
    13081 gtcggcgcgc tctcggcggt gttcagcaac gacatcatct gcctggcgat cgcgccggtg
    13141 ctggccgacg cctgccttgc gcggcggctc gaccccgtgc cctacctcct tggcctcgcc
    13201 tgcgcggcga acgtgggctc ggccgcgacg ctgatcggca acccgcagaa catgctcatc
    13261 gggcagacgc tcgcgctgtc gttctcgggc tacctggtgg aggccatcgc gcccgtgctg
    13321 ctcgggctgg cggcgacctg gggcatcctc gtgtggcaga cacgcggccg ctggcgcacg
    13381 gtgctcgacg acccgccgcc cgagcgccgc gaagactacg cggtgtacga ccgctggcag
    13441 accgccaagg gcctcggggt cgcgctggcg ctcgtcggcg cgttcctcct gtcggacgtg
    13501 cctcgcgaag tggccgcgct cgccggcgcc gggctgctgc tgatgagccg caggttccat
    13561 tcgcacaaga tgctcgggct ggtcgattgg gagctgctgg tgctgttcat cggcctgttc
    13621 gtcgtgaacc acgcgctggc gcaaaccggg attgccgccg ccgcgctcgc ctggctgagc
    13681 gacgcgggca cgccgctcac ggacaccggg ccgctgttcc tcgccacgct ggtgctgtcc
    13741 aacctcgtgt ccaacgtgcc cgccgtgatg ctgctgctgc cggcggcgac cgagccgttc
    13801 tcggggccga tgctcgcgct ggtcagcacg ctcgccggca acctgctcat cgtgggctcg
    13861 atcgcgaaca tcatcgtggt cgacgccgcg cgccgccgcg gcatcgccat cgactggcgc
    13921 acgcacgcgc gcaccggcgt tccggtcgcg ctggtcacgc tctgcgtcac cgcggtgtgg
    13981 ttcgcgctgc ggcactgagc gcgtcgggcg cgcccgacgc gcgccgcggt gccgcgtaaa
    14041 gcgtcacgaa cgccggccgg ccggccgacg gagtgcgcag tttgtcctta atgttcagcg
    14101 cgccgagtgg catcgtgagc gcgcttgacc tcgcccgaca tcacctcgcg gctgtcgacc
    14161 accggccagc tcctcgtgcg ctcggggatc gagatcggcc ggctgctcga cgcgatggca
    14221 caggaccgca gcgccgtgac ggcgcaggtg ccgccgcagc tgctgttcct gtcgcagctc
    14281 ctgtacgtcg agccggtgcg cggctacatg atgctgtcgt actccgacca caagcccgcc
    14341 aacagcgcgg cgctgtcggc acgctcgctc accttccgct gcaaccaccg cggcgcgcag
    14401 ttcgcgttca ccggcgacgg gccgcggcag tccgcgcaca aggggcaacc ctgcatccag
    14461 tgcgcgttgc cgagcgccgt gctcggcatg cagcggcgcg gcgccggccg cgtccaggtg
    14521 ccggcgcagg cgccggtgag ctgcgacctg cgcatgggcc tgatctcgtt cccgggccgc
    14581 gtggtcgacg tcagcctcga cggcatcggc acgctggtca ccgaccctgc gatcccgctg
    14641 tgcgccggca cgcggctcga gcgcgtgcgc atcagccatc cgcagtgcac gccgttcctg
    14701 atcgacttcg aaatccgcta cgtcacgcgc gtggtgctgg ccaacggcga gcacgccagc
    14761 cgcatcggct gccaggtggt cggcgcgcgc gagcgcctcg aagagctcat ccggctgttc
    14821 atcatcgacc ttgcctgacg cggggcggtg cccgagaatc cgggcaccgt ggactcgacg
    14881 ctgtacctga tcctcgccac gctgctgttc ctgggcatcc acgcgctgcc cgcgacgcga
    14941 ctgcgcgcgg ctgccgtgcg cgcgctcggc gagggcgcct accagggcct gttctcgctc
    15001 gcctcgctcg ccacgctcgt ctggatgagc ctggagtacg agcgcgcgcc cttcgaaggg
    15061 ctgtggccgg gactgcgcct ggtgccgatt gcggtgctgc cggtggcctt cgtgctcgtc
    15121 gcctgcggcg ccctcgcccg caaccctacc gcggcgggcc aggccggcgc gctccgccag
    15181 gaggacccgg cgcgcggcat catccgcgtc acgcgccacc cggtgatgtg ggccatcatg
    15241 ctgtgggcgg gcgcgcacct gctcgccatc ggctcgctgc aggcggtgat cttcttcggc
    15301 gggctgctgc tgctcgccgc cgccggcacc acgttgcagg acgcgcgcaa ggcagcgcag
    15361 ctcggcgagg actggaagcg tttcgccgca ctcacctcga acctgccgtt catggcgatc
    15421 gcgcaggggc gcaaccgcgt cgtctggcgc gagatcggct ggtggcggcc cgccgtcggc
    15481 ctggtcgcgt ttgccctgct actcgggctg cacgcctggc tgttcggcgt gcggccgtac
    15541 taaataggga cagacctcat ttttcttttc acatcgcgaa aagaaaaatg aggtctgtcc
    15601 ctatttagcg ggcaggtcga ggctggcgag cgccgcgtag accttgacga cggcggcgtc
    15661 gtcctccagg ccgtggccct gcgccgcggc ggcgaggtag agctggtgcg cggtcgcggt
    15721 gagcggcagc ggaaagccga acttcttcgc cgtgtcgagc acgatgccga gatccttgac
    15781 gaagatgttc accgccgaga gcggcgtgta gtcgcccgcc aggatgtgcg gcacgcggtt
    15841 ctggaacatc cacgaggacc cggcgctgtt cgagatcacc tcgtagagcg tcttcgggtc
    15901 ggcgccggcg cgcatgccga gcgccatcgc ctcgcaagcc gccgcgatgt gcacgcccgc
    15961 gagcagctgg ttgatcatct tcaccttcga gccgggcccg ggacgatcgc ccagccggta
    16021 caccttcgcc gcgatcgccg ccagcaccgg ctcgcacctc gcgaacgctt ccggcgcgcc
    16081 cgaggccatg atggtcatct cgcccgcggc ggccttcgcc gcgccgcccg agaccggcgc
    16141 gtcgatcatc agcaggccca tctgctcgag ccgcgcgccg aactgctcgg cgacgtccgg
    16201 cgccagcgtc gcgctcgaca ccacgacggc tcccttcctc atcgcggccg cggcgccctg
    16261 ctcgccgaac agcaccttct cggtctgctc ggcgttgacc accaggatca ccaccacttc
    16321 gcacgcggca ccaagggccg ccggcgtggc cgccgccttc gcgccctgcg cgacgaacgc
    16381 ctttaccacg tcggcgcgca cgtcgtagac gtgcgtctcg aacttcttcg ccagcagcga
    16441 cttcgccacg ccgccgccca tcgagcccag cccgatcacg ccgaccgcag tcatcacccc
    16501 tctcccttca gcttgcgctg cacgcgctcg tcgaacttgg cgacgttgac caccacgcgc
    16561 tcgtggctgc cgtcgccgat caggtccctt tcgtcgcgcg cctcgaggcg aaagcggatc
    16621 gtgcgcccct cgaccccggt cacctcggcc gtcgcggtga cgcgcatgcc gacgggtgtc
    16681 gccgcgatgt ggcgcacgtt gagcacggtg cccaggctct ggcgcccggg cggcagcagc
    16741 cgctccacgg cgtcgagcgc cgcggcctcg aaaagattga tcatcaccgg cgtggcgagc
    16801 acgcgcacgc ggccgctgcc gacgcgcggc gcggtatgct cctcggcgac catcagctcg
    16861 gcgcgaccgc gcagcccggg tttgagcaag tccatcgagg acctatcata gccttatgac
    16921 tccccgcgtc gtcatcctcg gctgcggttt cggcggcctg ttcgccgcac gcgcgctgcg
    16981 caaggcgccg gtggaggtca ccgtcgtcga ccgcaccaac caccacctgt tccagcccct
    17041 gctctaccag gtggcaaccg cgggtcttgc tgcgcccgcc atcgccgagc cgatccggcg
    17101 cgcgctggcg atgcaaagga acgtcaccgt gctctacggc gaggcgcagc gcgtcgacgt
    17161 cgcggcgcac gccgtggtcc tcgaaaacgg cgacacgctg ccctatgacc gcctgatcct
    17221 cgccaccggt gccaccgaca gctacttcgg ccacgacgaa tggcgcgcgc acgcgccggg
    17281 actgaagacg ctcgaggacg cgttcgagat ccgcagccgc atcctgctcg ctttcgagca
    17341 cgccgagcgc gaggcggatc ccgtgaagcg cgccgcgtgg ctcaccttcg tcgtcatcgg
    17401 cgcgggcgcc accggcgtgg agatggccgg catggtggcc gagatctcgc gccacacgct
    17461 gaagggcgag ttccgcaggt tcgatccgcg caacgcgcgc gtggtgctgg tcgagggtgc
    17521 ggatcgagtg ctgccgagct acacgccgga cctctcggag cgcgcgcgcg tgcagcttga
    17581 gcgcctgggc gtcaccgtgt ggctgggaag aaaggtgacg gacatcgacg cgcgaggcgt
    17641 gcagctcggc ggcgaccggc tcgaagccat gaccgtcgtg tggtgcgcgg gcgtcgccgc
    17701 gagcccgatc ggcgccacgc tcggcgtgcc gctcgcgcac ggcgggcgcg tgatcgtcga
    17761 gcccgacctg tcggtgcccg gtcacccgga aatccaggtg gtgggggacc tcgccttcct
    17821 gcccgagcac gagccgccgg tgcccggcgt ggcgcccgcc gccaagcaga tggggcggca
    17881 agcggcgcac aacatcgtcg ccgcgctcgc cagccggccg gcgcaagcgt tccgctaccg
    17941 cgactacggc cagctcgcca ccatcggccg cagcaaggcc gtggcgatgt tcggccgcat
    18001 ccacgtctgg ggctggctcg cctgggtggc gtggctcacc gcccacatct acttcctgat
    18061 cggcttcagg aaccggctgg tggtgctgat cgactgggcc tgggcctact ggaccttcga
    18121 gcgctccgcg cgcatcgtcg acaagggggc gcgcccgccg agctagccgc gcggcggtcc
    18181 gccccacagc cacgccgcgc cgcgcacgcc gctcgcatcg ccgtgcaccg gcggcagcag
    18241 ccgggtggcg acgagatccg agaagatgta gcgtccccag atcctgggca cgttgtcgta
    18301 caggcgccgc acgttcgaca ggccgccgcc cagcaccacg acgtccgggt cgatcaggtt
    18361 gatcaccgag ccgagcgcgc gcgccagccg ctcctcgtag cgcgcgagcg tggcgtcggc
    18421 gccgcggcgc acgatttcct cgggcgcaac ccgctcgccg gtagcgatct cgtggtcgcg
    18481 cgcgagcgcg ggccccgaca ggtaggtttc gatgcagccg gcgcgcccgc agtagcaggc
    18541 gggaagcggc aagtccccgg ccaccggcag cggcagcggg ttgtggcccc attcgccggc
    18601 gatggcgttg ggtccggtca gcacttcgcc gcgcaccacc acgccgccgc ccacgccggt
    18661 gcccaggatc acgccgaaca ccacctcggc gcccgcgccc gcgccgtcca ccgcctccga
    18721 cagcgcgaag cagttggcgt cgttcgcgat ccgcacttcg cggcccagcg catgctccag
    18781 gtcctgcttc aaggggtggc cgatgagctg cgtggagttg gcgttcttca ccaggccgct
    18841 cgccggcgac agcgcgccgg gaatgccgat cccgatcgtg cctgcgcccg cctcgcgcac
    18901 cagcgccgca accgccgcca gggtcgcctc gtaggcggcc cgcggcgccg ggacgcgctt
    18961 gcggaacact tcgcgtccct cgtcatccag caccagtgct tcgatcttgg tgccgcccag
    19021 gtcgatgccg atgcgcctcc cgccgctcac ccccctgccc cgcccgagtc gcagacggca
    19081 gtccacacgc cgccgtcctg ctcgatctcg ccggtcagcc gtgcgccgag ctcggcgaag
    19141 agcacgctgc aaacccgagt acggctggag gaatcggcca cggctggctg ctattctagc
    19201 gactgcccgc atggcacaag catcgatcgc cgccgactat gtcgtggtgg gcgccggcag
    19261 cgccgggtgc ctgctcgcca accgcctgtc gcgcgacccg gcggtgcgcg tcgtgctgct
    19321 cgaagccggc ggccgggacg attacttctg gatcgacatc ccggtcggct acctctacac
    19381 catcgccaac ccgcgcaccg actggtgcta ccagacctcg gccgacgagc acctcgacag
    19441 ccgctcgatc cactacgcac gcggccgcgg cctgggtggc tgctcctcga tcaacgccat
    19501 gatccacatg cgcggccaga aggcggacta cgaccactgg gcggcgctcg gcaaccgcgg
    19561 ctggagctgg gacgaggtgc tgccggtgtt ccagtctgtc gaggactacc agcacggcgc
    19621 ggtcgatacc tacggcgcgg gcggcgagct gcgcgtggaa gatccgcgtg tgcgctggga
    19681 gatcctcgac gcctggcgcg cagcggccgc cgaatgcggc atcgcgccaa tcaaggtgtt
    19741 caacaccggg gataacacgg gctgcgcgta tttccagatg aatcagaaga gaggcgttcg
    19801 ctggagcgcg accaaagcgt tccttcgtcc cgttctccac aggaagaatc tgacggttct
    19861 caccggcgcc catgtgacgg gactgcaatt cgaaggcagg gcgtgcaccg gggttacatt
    19921 tcgcgccggc cgcgacgcgc gctccgcaac ggcgtcgcgc gaggtgatcc tcgcggccgg
    19981 cgcgatcggg tcaccccaaa tcctgcagct ctcgggggtc gggcctggtg ctttgctgca
    20041 gcagcacgga attccggtgg ttcacgacct gcccggcgtc ggcgagaacc tgcacgacca
    20101 cctgcagatc cgcatgcagt accgggtcag cggcgtgaag acgctcaaca ccatggccaa
    20161 cagcctctgg ggcaaggcgg cgatggcgct cgagtacctc gcgttccgca ccgggccgct
    20221 caccatgccg ccgtcgcagc tcggcgcgtt ctcgaagagc gaccccgcgc agccgaccgc
    20281 caacatcgag tggcacgtgc agccgctgtc gctcgacaag ttcggcgagc cgctgcaccg
    20341 cttcccggcg atcacgccga gcgtgtgcaa tctgcgcccg gcctcgcgcg gctgggtgcg
    20401 catccgcgcc aacgaccctt tcacctaccc ggagatcaag ctgaactatc tttccgcgcc
    20461 ggaggaccgg caggtggcgg ccgacgcgat gcgcgccacg cgccgcatca tggcggccag
    20521 ggcgctggcg aagtaccggc cggaggaatt caagccgggg gcggcggcgc aaacacagga
    20581 ggcgctggaa caggccgcgg gcgagctcgg caccaccatc ttccatcccg tgggcacctg
    20641 cgccatgggc agcgtggtgg acgagcggct gcgggtgcgc ggcatcgggc ggctgcgcgt
    20701 gatcgacgcc gcggtcatgc cgcgcatcac ctccggaaac accaacgccc ccaccaccat
    20761 gatcgccgag aagggcgcgc gcatggtgct cgccgacgcc taaccggcgt tcagcgtcca
    20821 gtcgaactcg gtgtagcgca gccgcaggcc gaggccgtgg cgctcggcgg ccgcggcgac
    20881 gccgtcgtcc tgcacgtgcg ccaggatgaa ttcgagcacg ccgcgcctgc cgccgaagtc
    20941 caccgcgtac cagtcgaaga gcttcggcac ctcgagcgtg gcgccgccgg ccgcaaggcg
    21001 aacgtgcgac gcgaggtagg cgcaggtggc gcgctccagg tcgccggtca ggtcttcggc
    21061 cgcgtaggcc gcaggcgcgg gcgacgagcg gcaggcggag tacatggcga aatgcacgcg
    21121 ctcgtcgaac atgtacggcg caagcgcgta gcgcggcccg tcgcgccgca gcggcgtcgc
    21181 gccgaaggcg aggcgcggcg cgttcacccg cagcagcccg tgctcgatct cgtccagcga
    21241 gaagacgtgc ccgccgacca tgtactgcga gccgctgaag aagtcgccca gcgcgcgcac
    21301 cccggcaagc gcgcggcgcg ccaccaccgc gtgcagtacg agcgcgttgt agacgttgag
    21361 ccagaaggcg agccgcaagc cgatatgcag cgatgcgcag tcgaagtcgg cgagcgcccg
    21421 cgcggccacg cccagttccg cgtactcggg cgactcggcg aagcgcgcgt agtcgaagac
    21481 atggcctgcc ggctcgtaat gcgcggcgca ggccgcgtgc agcgccgcga ccagcagtgc
    21541 cggggcgtcg tccgaccggg cgtgcgagtg ctgctcgatg ggattgagca ccggcatcgc
    21601 gcacggccgc ccggcggcta ggggacgagc tcctcgtcgt cgtgctgcgg ctcggcctgg
    21661 gcctgtgaca gcgagcgcag ccggaagcgc ccttcgtagt cgaacagcca cgtctccgtg
    21721 aacgtgacgc tgctcccgcc gtccaggagc ttcatcgagg ggcgcgggaa cggctgcgcc
    21781 tggcgcaggg tctgtgcgac gagctcgggc agccacgggt tgtgccacgg ggcgcgcacc
    21841 aggtccaggc gccgcgcctc gcccagcacg tcgatctcgg cgcgcatgac gatgaccgcg
    21901 cgcaacgggt tcggcggcgg cccctcgaac accctgcccg cgttggccgc gtgcagcagc
    21961 ccggcaattt cgcgctggta ctcggcgagc gtcgcggcgc ccgaggccgg cggcagcggg
    22021 atcgaggcct gcggcatgct gtggtaggtc ttggccaccg ccgcgggaac gtcaagcggc
    22081 ggtggcgccg cgcaggcggc gagcagcagc gcgagcgccg caagggcttg aaaaccatgc
    22141 ccgcggcccc cagcttgcgc taccatcccg cagcattttt ccggcggcat gaggagagat
    22201 cataaatgaa gcgcgtcgtc ctgtttttgg ccaccaacat cgcggtgctg ctgctgctgt
    22261 cggtggtgat cagcgtgctc gggctggacc gctggctcgc ggccgaaggc atcaattacc
    22321 aggggctgct ggtgttctcg gcggtgatcg gcttcggcgg cgccttcatc tcgctcgcga
    22381 tgtccaagac catggcgaag tggtccaccg gcgcgcacgt catcgacggc accgagggca
    22441 ccaccgagta ttggctggtg cagaccgtga agcgcctggc cgagaaggcc ggcatcggca
    22501 tgcccgaggt ggcagtgtat gaaggcgcgc ccaacgcgtt cgccaccggt gcgttcagga
    22561 actcctcgct ggtcgccgtc tccaccggcc tggtgcagtc gatgagcaag gaagaagtgg
    22621 aagccgtgct cggccacgag atcggccacg tcgccaacgg cgacatggtg acgctcaccc
    22681 tgatccaggg cgtggtcaac accttcgtga ttttcctgtc gcgcgtggtc gcctttttcg
    22741 tcgacaaggt gatcttccgc accgagcgcg gcgtcggccc gggcttctac atcaccgcca
    22801 tcgtgttcca gatcgtgttc ggcgtgctcg cctcgatcat cgtcgcctgg ttctcgcgcc
    22861 agcgcgaatt ccgggccgat gcggcctcgg ccgacctgct cggcaatccg cgccccatga
    22921 tgaacgcgct ggcgcgcctg ggcgggctcg agccgggtgc gctgccgcag tcgatgaagg
    22981 ccgcgggcat caccgacaag ccctcgggct tcgccgcgct gttctcgacg cacccgccgg
    23041 tcgaggagcg catcgccgcg ctgcaggcgc gatccgccgg ccgggcgtaa cgcccgttcc
    23101 accctacctg ctacgattcc ggtcatgaag atcggaatcg tcggcgtcag cggctacggc
    23161 ggcagcgagc tgctgcgcct ggccgccggc caccccgcgt tcgagctcgc ctacgtcggc
    23221 ggcgagtcca ccgccgggca gacgctcgcg cagcgttttc ccgcgctcgc cggccatgcc
    23281 gccggcaagc tcgtcatcca gccgttcgtg ccggagacga tctcgggcat cgagctgctg
    23341 ttcgcctcgc tgcccaccgg aaaatcgcgc gagccgctcg cccgcgtggc caaggacatc
    23401 cgcatcgtcg acatcggcgg cgaccaccgc ttcgtcgagg gctggaccta cgggctcacc
    23461 gagctccctg gccagcgcga caagatccgc ggcgcgcatc gcctcgccaa cccgggctgc
    23521 tacccggccg cgtcgctgct cgcactcgcc ccgctcgtca gggaaggcct gatcgagccc
    23581 gagggcatcg tc
//
LOCUS       DBNHKE010000066         3207 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_116920, whole genome shotgun sequence.
ACCESSION   DBNHKE010000066 DBNHKE010000000
VERSION     DBNHKE010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_116920"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16O9_04315"
     CDS             <1..208
                     /locus_tag="AC16O9_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6515875.1"
                     /translation="ARHVPINELPARLNELDPDAEMVAICHHGVRSLHVVHFLARQGF
                     ERVHNLAGGVDAWARAIDPGMPVY"
     gene            222..1547
                     /locus_tag="AC16O9_04320"
     CDS             222..1547
                     /locus_tag="AC16O9_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR6515876.1"
                     /translation="MIRRLALALFACAPLAAAAQNLTQVYQDARGYDAQYGAARQALQ
                     AGLERVPQGRALLLPSLNLTANTTRSHLESDSKNEVIQPSFERDVNSYGFALTFTQPL
                     YRRQNWLQADQAELQARQAEFTFGQAGQDLILRAAQAYFDVLAAQDTLALVRAQKAAI
                     AEQLAQAKRNFEVGTATITDTHEAQARYDLAVAQEISAQNDVEVRLRALQQLTGKEYS
                     ELKALRPDVKLAPPNPNDMQAWVNLAEKQSYSVLAQNAAVEIARLEAQRNSAAHHPTL
                     DLVASHSYTSQSASQLSATGSEVNNSSVALQLSVPLYQGGGLTSREREAAYNLERTRQ
                     ELENARRTSALATRQAYLAVINGIAQISALDQALVSTQSALQSNRLGYEVGVRINIDV
                     LNAQQQVFSTRRDLAVARYNTITNFLRLKAAAGGLREEDLEEVNRALAQ"
     gene            complement(1525..2784)
                     /gene="waaA"
                     /locus_tag="AC16O9_04325"
     CDS             complement(1525..2784)
                     /gene="waaA"
                     /locus_tag="AC16O9_04325"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIR6515877.1"
                     /translation="MRTLYSWLLYAALPWILARLWWRGRREPGYREAIGERFGWSELE
                     PRPRLLWLHAVSVGEVRAGAPLVRALQREYPDHDVLITCLTASGRETVKQVYGESVLL
                     AYLPYDYPRAVRRFLEHFRPRLGVLVETEIWPNLIDACRQYNVPVMLANARMSQRSAR
                     GYQRLGWLTRPVFAALAATCAQGPADARRLALLGANGVSVAGSLKFDIEPDPAKAQEG
                     RALRATLGGRKVLLFASTRDGEEARLLEASGAADDGTLLVLVPRHQQRFDAVAALAER
                     LGFAVARRSRGEAPRTGRSLYLGDTMGEMAFYYALADVAVIGGSFEPLGGQNLIEACA
                     AGVPVIAGPHMFNFADVTRLAVRAHAAVQVSDAAAALGAARSLLADAERRTRMGDAGK
                     RLCERHRGAAERHLAVCQRLLTAPGRG"
     gene            complement(2863..>3207)
                     /locus_tag="AC16O9_04330"
     CDS             complement(2863..>3207)
                     /locus_tag="AC16O9_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6515878.1"
                     /translation="DMGEPVRIVDLARQLIRLSGYAEGDILVEYTGLRPGEKLYEELL
                     AGAETTLPTPHPKLRVARANSGADAALLEEVLGWIAQAPDPGPEAVRARLRAWLPEYA
                     PAEDATTRGSSA"
BASE COUNT          532 a         1189 c         1080 g          406 t
ORIGIN      
        1 cgcgcgccac gtgccgataa acgaattgcc cgcgcgcctg aacgagctcg atccggacgc
       61 cgagatggtc gcgatctgcc accacggcgt gcgcagcctg cacgtcgtgc acttccttgc
      121 gcgccaaggc ttcgagcgcg tgcacaacct cgccggcggc gtcgatgcct gggcgcgcgc
      181 gatcgaccca ggcatgcccg tctactgaga tccgacgccc catgatccga cgcctcgccc
      241 tcgccttgtt cgcctgcgct ccgctagccg cggcggcgca gaacctgacc caggtctacc
      301 aggacgcccg cggctacgac gcgcagtacg gcgccgcacg ccaggcgctg caggcggggc
      361 tcgagcgcgt gccccagggg cgcgcgctgc tgctgcccag cctcaacctg acggcgaaca
      421 cgacgcgctc gcacctcgaa tcggattcga agaacgaagt catccaaccc agcttcgagc
      481 gcgacgtcaa ctcgtacggc ttcgcgctca ccttcaccca gccgctgtac cggcggcaga
      541 actggctgca ggccgaccag gccgagctgc aggcaaggca ggccgagttc accttcggcc
      601 aggcggggca ggacctgatc ctgcgcgccg cgcaggccta cttcgatgtg ctcgcggcgc
      661 aggacacgct cgccctggtg cgggcgcaga aggccgccat cgccgagcag ctggcgcagg
      721 ccaagcgcaa cttcgaggtc ggcacggcga ccatcaccga cacgcacgaa gcgcaggcgc
      781 gctacgacct cgccgtggcg caggagattt cggcacagaa cgacgtcgag gtacgcctgc
      841 gcgcgctgca gcagctcacc ggcaaggagt acagcgagct gaaggcgttg cggcccgacg
      901 tcaagctcgc gccgccgaac ccgaacgaca tgcaggcctg ggtgaacctc gcggaaaagc
      961 agagctacag cgtcctggcc cagaacgcgg cggtcgagat cgcccggctc gaagcgcagc
     1021 gaaacagcgc cgcccaccat ccaacgctcg acctggtggc gagccacagc tatacgtcgc
     1081 agagcgcctc gcagctctcc gcgacgggca gcgaggtgaa caacagctcc gtcgcgctgc
     1141 aactctcggt gcccctgtac cagggcggcg gcctcacctc gcgcgagcgc gaggcggcct
     1201 acaacctgga acgcacccgc caggagctgg agaacgcgcg gcgcacttcc gcgctcgcca
     1261 cgcgccaggc ctacctcgcg gtgatcaacg gcatcgccca gatcagcgcg ctcgaccaag
     1321 cgctcgtctc gacgcagagc gcgctgcagt cgaaccgcct gggctacgaa gtgggcgtgc
     1381 gcatcaacat cgacgtgctg aacgcgcagc agcaggtgtt ctccacgcgc cgcgacctcg
     1441 ccgtggcgcg ctacaacacg atcaccaact tcctgcgcct caaggccgcc gccggcgggc
     1501 tgcgcgagga agacctcgag gaagtcaacc gcgccctggc gcagtaagca ggcgctggca
     1561 taccgcgagg tggcgctctg cggcgccgcg atgccgctcg cacaggcgct tgccggcatc
     1621 gcccatgcga gtgcggcgct cggcatcggc cagtagcgaa cgcgccgcgc ccagcgccgc
     1681 ggcggcatcg ctcacctgca ccgctgcgtg cgcccgcacc gccaggcgcg taacatcggc
     1741 gaagttgaac atgtgcggcc ccgcgatcac cgggacgccg gccgcgcagg cctcgatcag
     1801 gttctgcccg cccagcggct cgaagctgcc gccgatcacg gccacatcgg cgagcgcata
     1861 gtagaacgcc atttcaccca tcgtgtcgcc gagatagagg ctgcggcccg tgcgtggtgc
     1921 ttcgccgcgg ctacgccgtg ccacggcgaa tcccaggcgc tcggcaagcg cggccaccgc
     1981 atcgaagcgc tgctggtgcc ggggcaccaa gacgagcagc gtgccatcgt ccgcggcgcc
     2041 gctggcctcg agcaaccgcg cttcctcgcc atcgcgcgtg ctggcaaaga gcaggacctt
     2101 gcgcccgccg agcgtggcgc ggagcgcgcg cccctcctgc gctttcgccg gatcgggctc
     2161 gatgtcgaac ttcaggctgc cggcgacgct gacgccgttc gccccgagca aggcaaggcg
     2221 ccgcgcgtcg gccggaccct gcgcgcacgt ggccgcgagc gcggcgaaga ccggccgggt
     2281 cagccagccg agccgctggt agccgcgcgc cgagcgctgc gacatgcgcg cgttggcaag
     2341 catgacgggc acgttgtact gacggcaggc gtcgatcaga ttcggccaga tttcggtttc
     2401 gacgaggacg ccgaggcgcg ggcggaaatg ctcgagaaag cgccgcacgg cgcgcgggta
     2461 gtcgtaaggc aggtaggcga gcagcaccga ctcgccgtac acctgcttca cggtctcgcg
     2521 cccgctcgcg gtgaggcagg tgatcagcac gtcgtgatcc ggatactcgc gctgcagcgc
     2581 gcgcacgagc ggcgcgccgg cgcgcacctc gcccaccgac accgcgtgca gccagagcag
     2641 gcgcggcctg ggctcgagct cgctccagcc gaagcgctcg ccgatcgctt cccggtagcc
     2701 cggctcgcgc cggccgcgcc accacaggcg cgcgaggatc cacggcagcg ccgcgtacag
     2761 cagccaggaa tagagcgtgc gcaaggtgcc ctatgataag accatgcgag tgcgcgccag
     2821 cgacgccact cgccgagggc cttgggcgct tcgcggactg gcttaagcgc tactaccgcg
     2881 tgtcgtagcg tcctcagcgg gggcatactc cggcagccag gcgcgcagcc gcgcgcgcac
     2941 cgcctccggc ccggggtcgg gtgcctgcgc gatccagcca agtacttcct cgagcagcgc
     3001 cgcatccgcg ccagaattgg cgcgcgccac gcgcagcttc ggatgcggcg tgggcagcgt
     3061 ggtctcggcc cctgcaagca gctcctcgta gagcttctcg ccgggccgca ggcccgtgta
     3121 ctcgacgagg atgtcgccct ccgcgtagcc cgagagccgg ataagctggc gcgcgaggtc
     3181 gacgatgcgc accggctcgc ccatgtc
//
LOCUS       DBNHKE010000067         5076 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_381439, whole genome shotgun sequence.
ACCESSION   DBNHKE010000067 DBNHKE010000000
VERSION     DBNHKE010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_381439"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16O9_04335"
     CDS             complement(<1..261)
                     /locus_tag="AC16O9_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515879.1"
                     /translation="MAPSRPTAFAVAAAALLAAACVTAPSGPSVLVLPGSAKSFEQFR
                     ADDYECRQYAGFQVNGTTPERAAEQSGVKSAAIGTAVGAVAGG"
     gene            complement(228..563)
                     /locus_tag="AC16O9_04340"
     CDS             complement(228..563)
                     /locus_tag="AC16O9_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515880.1"
                     /translation="MKRALILVAAAAFAALAMAGPADAHRGARVRLGVVIGGPLWWGW
                     HQPYYPHYTERVIVERPGPTVYVEKDADGVERDADQYWYYCPDSETYYPYVKQCASPW
                     HRVAPQPSP"
     gene            632..1744
                     /locus_tag="AC16O9_04345"
     CDS             632..1744
                     /locus_tag="AC16O9_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6515881.1"
                     /translation="MRIRVIGAGPAGLLFALLAKRRFPDWDIAVTEQNPPDATFGFGV
                     VFSQGALAFLERDAPDLHAALAARMESWPMQRIVHRGEAVDIDGNGFSAIARLQLIQF
                     LQALCRAEGVGLAFGRRLDALPEADLVVGADGLNSVVRGAPGFAPKIEWLTNRFVWYG
                     TRQRFECLTLTFRDSAQGAFVAHHYRYSPEMSTFIVECDAATWQRAGLDRMGDAESRA
                     YCERVFAADLGGHALVSNNSTWRQFPLLSNAVWTAGNRVLIGDALRSMHFSIGSGTRL
                     AFEDAIALDRALGEAGEDVPAALAAFERERRPVVEKLFTAAAASSWWYEEFPARMARF
                     DALRLAYDYMTRSGRMSDERLCALAPNFMSRIKKGP"
     gene            1776..2372
                     /locus_tag="AC16O9_04350"
     CDS             1776..2372
                     /locus_tag="AC16O9_04350"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-type transcription factor C-terminal
                     domain-containing protein"
                     /protein_id="HIR6515882.1"
                     /translation="MVQILNVSSARQPSARVRQRNEARRLDILRAAARVFRRRGIAAA
                     GMREIAEEAGLSPGNLYHYFSGKDEILLFCQERTLERMQAAVDAARGTPAERLRAVLR
                     AHVHCMLDELEGATAHLDVEALPKKLRAAMIERRDAYERAVRAIVAEAMPRGDAALVT
                     RAMLGAVNWTARWYRPDGAQAPDEIAETLSDYLARGLA"
     gene            2369..2851
                     /locus_tag="AC16O9_04355"
     CDS             2369..2851
                     /locus_tag="AC16O9_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6515883.1"
                     /translation="MNRVRMTLRVNGEEQEVLFHSYKTLLEVLREELGLTGTKHGCEL
                     GECGACAVLVDGAPVLSCLELALQCEGRDIQTVEGLAQGTELHPLQAAFADHGGSQCG
                     YCTSGMLMTAKALLDREPAPSRERIKEAISGNLCRCTGYQQIWEAIESAARRLREAKP
                     "
     gene            2848..>5076
                     /locus_tag="AC16O9_04360"
     CDS             2848..>5076
                     /locus_tag="AC16O9_04360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6515884.1"
                     /translation="MNAPGKPGGKTRFDTIGKPRRRVDGRAKAIGELRFADDLSLPRM
                     LHCKLLRSPHPHALIESIEVARAQAHPGVHLVLTGAQFPVPYGIMPVSQDEHPLARER
                     VRFVGDPVAAVIATDEQTAVAALELIEVKYRPLDTIASAEEGLAKPEPRIHDYGERGN
                     IHRNQAYEFGDVDAALAKSAHVFEDLFFYEGNTHLPMEQQASLASIEGGGTLLLQTST
                     QNPHYLHRQLARVLQVPAARIRVIATPNGGGFGGKCDPGNHEFVVAKAALMLGRPVKI
                     CLTREEVFYQHRGRHPVLMKFRTGVDAGGKLTAMHLQTLLDGGAYGSHGPASTFYTGV
                     LTPVTYELPRFKFEACRVFTNKPACGPKRGHGTPQPRFGQEVQLDKIAEKLGLDPAEL
                     RLAMVTQPDSLTASWLRVGSTGLAECIRKVVEMSGWKARRGKLGPGRGLGLACSAYMC
                     GAGVSIYWNKLPHSAVQLLLDRSGQVTVYCGATELGQGSDDVLASLIAEALGIDPANV
                     RCITGDTGITPIDLGSYSSRVTIMMGNAALQAAGRVREQLAAAAAEQLNTLPERVVFS
                     QDRVFSAENPGGAMSFIEAVALAEAKFGTLGALGSYAPPKAPGRYKGAGVGPSPAYSF
                     SACVVETEVDAQTGWVTVPKVWIAHDIGRALNPVLARGQVIGGVYMGLGEALMEEQAF
                     RRLPPKLSGALVHRQPSILEYKSLTSLDMPEVEVALIEDPDPNCPYGAKEVGQGPLLP
                     VMP"
BASE COUNT          752 a         1766 c         1816 g          742 t
ORIGIN      
        1 gccgcccgcg accgcgccca ccgcggtgcc gatggcggcg ctcttcacgc cgctttgctc
       61 tgcggcgcgc tcaggcgtgg tgccgttcac ctggaagccg gcatactggc ggcattcgta
      121 gtcgtcggcg cggaactgct cgaagctctt cgcgctgccc ggcagcacca gcaccgacgg
      181 gccgctcggc gcggtgacgc acgcggcggc gagaagcgcc gctgccgcta cggcgaaggc
      241 tgtggggcga ctcggtgcca tgggctcgcg cactgcttga cgtacgggta ataggtttcg
      301 gaatccgggc agtagtacca gtactggtcg gcgtcgcgct cgacgccgtc ggcatccttc
      361 tcgacgtaca cggtggggcc cgggcgctcg acgatcacgc gctcggtgta gtgcgggtag
      421 tagggttgat gccagcccca ccacagcggc ccgccgatca cgacgcccag gcgcacccgt
      481 gcgccgcgat gggcgtcggc aggcccggcc attgccagcg cagcgaatgc cgcggccgcc
      541 accagtatca acgctcgctt catgttgtga agactagacc caagcgccgc gcgcctgggt
      601 aacaaggcgt aacaatttgt atcatcggcc gatgcgcatc cgcgtcatcg gcgccggccc
      661 cgccggcctg ctgttcgcgc tgctcgccaa gcggcgcttt cccgattggg acatcgccgt
      721 caccgagcag aatccgcccg acgccacctt cggcttcggc gtggtgttct cgcagggggc
      781 gctcgccttc ctcgagcgcg atgcgccgga cctgcacgcc gcgctcgccg cgcgcatgga
      841 atcgtggccg atgcagcgca tcgtgcaccg cggcgaggcg gtcgacatcg acggcaacgg
      901 tttttccgcc atcgcgcggc tgcagctcat ccagttcctg caggcgctgt gccgcgccga
      961 gggcgtcggc ctggcgttcg ggcgccgcct cgatgcgctg ccggaggcgg acctggtggt
     1021 cggcgccgac ggcctgaact ccgtcgtgcg gggcgcgccg ggctttgcgc cgaagatcga
     1081 gtggctcacc aacaggttcg tctggtacgg cacgcgccag cgcttcgagt gcctgacgct
     1141 cactttccgc gacagcgccc agggggcctt tgtcgcccac cactaccgct acagcccgga
     1201 gatgagcacg ttcatcgtcg agtgcgacgc ggcgacctgg caacgcgccg gcctggaccg
     1261 catgggcgac gccgaatcgc gcgcttactg cgagcgcgtg ttcgcggcag atctgggcgg
     1321 ccacgccctg gtgtcgaaca actcgacctg gcggcagttc ccgctgcttt ccaacgcggt
     1381 ctggactgca ggcaaccgcg tgctgatcgg cgacgcgctg cgcagcatgc acttctccat
     1441 cggctcgggg acacgcctcg ccttcgagga cgcgattgcg ctcgaccggg cgctcggcga
     1501 ggcgggcgaa gacgtgccag cggcgcttgc ggccttcgag cgcgagcgcc ggccagtggt
     1561 ggagaagctg ttcaccgcgg ctgccgcaag ctcctggtgg tacgaggaat tcccggcgcg
     1621 catggcccgc ttcgacgcgc ttcgccttgc ctacgactac atgacccgca gcggccggat
     1681 gagcgacgag cggctttgcg ccctggcgcc gaatttcatg agccggatca agaaagggcc
     1741 ttgatttcca cgtggttagg aatagcattc gaacgatggt tcaaatcttg aacgtgagtt
     1801 ccgcgcgcca gccctcggcg cgggtgcgcc agcgcaatga agcccgtcgc ctggacatct
     1861 tgcgcgccgc agcgcgcgtg ttccgccggc gcggcatcgc cgcggccggc atgcgcgaga
     1921 tcgccgagga agcggggctt tcgcccggaa acctttacca ctacttctcg ggcaaggacg
     1981 agatcctgct gttttgccag gaacgcacgc tcgagcgcat gcaggccgcg gtggatgcgg
     2041 cacgcggcac gccggccgag cgcctgcgcg cggtgctgcg ggcgcacgtg cactgcatgc
     2101 tcgacgagct ggagggcgcc accgcgcacc tggacgtcga ggcgctgccg aagaagctgc
     2161 gcgcggcgat gatcgagcgg cgcgacgcct acgagcgcgc ggtgcgcgcg atcgtcgccg
     2221 aggcgatgcc gcgcggcgac gcggcgctcg tgacgcgcgc catgctgggc gcggtgaact
     2281 ggaccgcgcg ctggtaccgg ccggacggcg cgcaggcgcc ggacgagatc gccgagacgc
     2341 tttcggacta tctggcgagg gggctggcat gaaccgggta cgcatgacgc tgcgcgtgaa
     2401 cggcgaggag caggaggtcc tgttccattc gtacaagacg ctgctcgaag tgctgcgcga
     2461 ggagctcggg ctcaccggca ccaagcacgg ctgcgagctc ggcgagtgcg gcgcctgcgc
     2521 cgtgctggtg gacggcgcac cggtgctctc ctgcctcgag ctcgcgctgc agtgcgaggg
     2581 acgtgacatc cagaccgtcg agggcctggc gcagggcacg gagctgcatc cgctgcaggc
     2641 ggcgttcgcc gaccacggcg gctcgcagtg cggctactgc acctcgggca tgctgatgac
     2701 cgccaaggcg ctgctcgacc gcgagcccgc gccgagccgc gagcgcatca aggaagcgat
     2761 ctcgggcaac ctctgccgct gcaccggcta ccagcagatc tgggaggcga tcgagtcggc
     2821 ggcgcgccgc ctgcgggagg ccaagccatg aacgcgcccg gcaaacccgg cggcaagacg
     2881 cgcttcgaca ccatcggcaa gccgcggcgg cgcgtcgacg gccgcgccaa ggcgatcggc
     2941 gagctgcgct tcgccgacga cctctcgctg ccgcgcatgc tgcattgcaa gctgctgcgc
     3001 tcgccgcacc cgcacgcgct gatcgagtcg atcgaggtcg cgcgcgcgca ggcgcatccg
     3061 ggcgtgcacc tggtgctcac cggcgcgcag ttcccggtgc cctacggcat catgccggta
     3121 tcgcaggacg agcacccgct cgcgcgcgag cgcgtgcgct tcgtcggcga tccggtcgcg
     3181 gcggtgatcg cgaccgacga gcagaccgcg gtcgccgcgc tcgagctgat cgaggtgaag
     3241 tatcggccgc tcgacaccat cgcgtcggcc gaggaggggc tggcgaagcc cgagccgcgc
     3301 atccacgact acggcgagcg cggcaacatc caccgcaacc aggcctacga gttcggcgac
     3361 gtcgacgcgg cgctggcgaa gtcggcgcac gtgttcgagg acctgttctt ctacgaaggc
     3421 aacacccacc tgccgatgga gcagcaggcc tcgctcgcct cgatcgaggg cggcggcacg
     3481 ctgctgctgc agacgagcac gcagaatccg cactacctgc accgccagct cgcgcgcgtg
     3541 ctgcaggtgc cggcggcgcg catccgcgtc atcgccacgc cgaacggcgg cggcttcggc
     3601 ggcaagtgcg atcccggcaa ccacgagttc gtggtggcca aggccgcgct gatgctcggc
     3661 cgcccggtga agatctgcct cacgcgcgag gaggtgttct accagcaccg cggccggcac
     3721 ccggtgctga tgaagttccg taccggcgtg gacgcgggcg gcaagctcac ggccatgcac
     3781 ctgcagacgc tgctggacgg cggcgcctac ggctcgcatg gaccggcgag cactttctat
     3841 acgggcgtgc tcacccccgt gacctacgag ctgccgcgct tcaagttcga agcctgcagg
     3901 gtcttcacga acaagcccgc ctgcggcccc aagcgcggcc acggcacgcc gcagccgcgc
     3961 ttcggccagg aggtgcagct cgacaagatc gccgagaagc tcggcctcga tccggcggag
     4021 ctgcgcctcg cgatggtcac ccagcccgat tcgctcaccg cgagctggct gcgcgtgggc
     4081 tcgaccgggc tcgccgagtg catccgcaag gtggtcgaga tgtcgggctg gaaggcgcgc
     4141 cgcggcaagc tcgggcccgg gcgcggcctc gggctcgcct gctcggcgta catgtgcggg
     4201 gcgggggtct cgatctactg gaacaagctg ccgcactcgg ccgtgcagct gctgctcgat
     4261 cgcagcggcc aggtcaccgt gtactgcggc gccaccgagc tcgggcaggg gtcggatgac
     4321 gtgctcgcct cgctcatcgc cgaggcgctc ggcatcgacc ccgcgaacgt gcgctgcatc
     4381 accggcgata ccggcatcac gcccatcgac ctggggtcct attcgagccg cgtgacgatc
     4441 atgatgggca atgccgcgct gcaggcggcg ggccgcgtgc gcgagcagct cgccgcggcg
     4501 gcggccgagc agctgaacac gctgcccgag cgcgtcgtgt tctcgcagga ccgcgtgttc
     4561 agcgcggaga acccgggcgg cgcgatgagc ttcatcgagg cggtggcgct ggccgaagcg
     4621 aagttcggca ccctgggcgc gctgggctcg tacgcgccgc cgaaggcgcc cggtaggtac
     4681 aagggcgccg gcgtcgggcc gtcacccgcc tacagcttct cggcctgcgt ggtggagacc
     4741 gaggtggacg cgcagaccgg ctgggtgacg gtgcccaagg tctggatcgc gcacgacatc
     4801 gggcgggcgc tcaatcccgt cctggcccgc gggcaggtga tcggcggcgt ctacatgggc
     4861 ctgggcgagg cgctgatgga ggagcaggcg ttccggcggc tgccgcccaa gctctcgggc
     4921 gcgctggtgc accgccagcc ctcgatcctc gaatacaaga gcctcacctc cctggacatg
     4981 cccgaggtcg aggtggcgct gatcgaggac ccggatccga actgcccgta cggcgcgaag
     5041 gaagtggggc aggggccgct gctgccggtg atgccg
//
LOCUS       DBNHKE010000068        21753 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_593047, whole genome shotgun sequence.
ACCESSION   DBNHKE010000068 DBNHKE010000000
VERSION     DBNHKE010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 21753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_593047"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="AC16O9_04365"
     CDS             complement(<1..179)
                     /locus_tag="AC16O9_04365"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443240.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR6515885.1"
                     /translation="MREAGVKEKGRAKTARIPVKIVSKPALPKPAWIRVRAPALGSRF
                     TEIKGILREQKLHTV"
     gene            complement(172..798)
                     /gene="lipB"
                     /locus_tag="AC16O9_04370"
     CDS             complement(172..798)
                     /gene="lipB"
                     /locus_tag="AC16O9_04370"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR6515886.1"
                     /translation="MEAALATRLKSLGLAEYGAVAARMREFTLQRDADTPDELWIVEH
                     PPVYTLGQGADPAFGPKQANGIPVVQSERGGEITYHGPGQAIVYTLVDLARRGIKVKA
                     FVHLLEQCVINFLVRHEVQAERKAGAPGVYVGGAKIASLGIRVTRGRAWHGIALNVDM
                     DLTPFQAIDPCGYPGLSVVQARDFGVLGTAKEVGIRLARLIAQRLEHA"
     gene            complement(800..1075)
                     /locus_tag="AC16O9_04375"
     CDS             complement(800..1075)
                     /locus_tag="AC16O9_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HIR6515887.1"
                     /translation="MMPIQNEETLLSYPTDFPIKVMGRKEKRFAATVLGIVRKHAPDF
                     DPATMELRPSRQGTYLSVTCTIRATSREQLDELYRDLCDHPSVVMVL"
     gene            complement(1072..1932)
                     /locus_tag="AC16O9_04380"
     CDS             complement(1072..1932)
                     /locus_tag="AC16O9_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HIR6515888.1"
                     /translation="MVFLNGRFLPLEQATVPVLDRGFIFGDGVYELIPVYSRAPFRMD
                     EHLARLERSLAAVRIANPYARDKWRSIILELVAKQPWEDQGVYLQVTRGVAKRDHAFP
                     AGVEPTVFAMSNPLVNPPQELVEKGVAAVSAPDNRWLRCDIKSISLIGNVLLRQASAD
                     AGAAETILFRDGILTEASASNVFVAKNGVVLSPPKSNLILPGITYDVVVEIAQQLGLA
                     LEFRDIRESEVRAADELWVTSSSKEILAVVELDGKPVGEGRPGPLFRRAWEGYQEFKR
                     RVMRKEAVSA"
     gene            complement(1946..3073)
                     /locus_tag="AC16O9_04385"
     CDS             complement(1946..3073)
                     /locus_tag="AC16O9_04385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR6515889.1"
                     /translation="MRAALIASAFLFVAWGASAQAPQPPAVAARAFQVLDLTSGQVLA
                     ADKADERVEPASLTKLMTAYLVFGALREGRLTPEQQVQVSAHARQAPGSRTFLEEGSS
                     VSVDTLVRGMVVQSGNDACIALAEALAGTEARFAEQMNREAARLDLRDTRFVNATGLP
                     HPQHYSTARDLALLAAALIRDYPEQYARYYAMKEFRHNDITQMNRNRLLWLDPSVDGV
                     KTGFTEAAGYCLIASSERNGRRLVSVLLGADSEAARARESQKLLNWGYQGFDGVRLYE
                     AGQPVKAIHVWKGAHEAVRAGFERGLVVTVPKGEAKLLAAELLTHPPVLAPVQKGDKV
                     GVLRVALDGRPLGEYPVVALESVPQAGILGRAWDTLRLWLK"
     gene            complement(3109..3618)
                     /locus_tag="AC16O9_04390"
                     /pseudo
     CDS             complement(3109..3618)
                     /locus_tag="AC16O9_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(3615..4877)
                     /locus_tag="AC16O9_04395"
     CDS             complement(3615..4877)
                     /locus_tag="AC16O9_04395"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR6515890.1"
                     /translation="MKLLVKGGRVVDPATGRDAVGDVSIADGKIVAAPGGKPDRVIDA
                     QGLVVAPGLIDLAARLREPGYEYKATLESEMLAAVAGGVTSLACPPDTDPPLDEPGLV
                     DMLRRRAKALARARVYPVGALTVKLEGRQLTEMAELAEAGCVAFSQADAAIADTQVLW
                     RALQYAATFGFPVWLRAEDLWLAQGGVAHDGEIATRLGLAGIPAFAETVALATLLELV
                     RATGARVHVCRISSAAGVAMLRRARHDGLPLTCDVGVHHVHLSDMDLGYFDAQCRLEP
                     PLRSSRDRDALGAGLADGTIDCIVSDHTPVDEDRKHLPFAEAEPGASGLELLLPLALK
                     WGVQAKLNLAQTLARVTIAPARILGVDAGRIAPGAPADLIVFDPEAPFRVSASALKSQ
                     GKNTPFLGLELAGRVRATLVDGQVVYEA"
     gene            complement(4874..5827)
                     /locus_tag="AC16O9_04400"
     CDS             complement(4874..5827)
                     /locus_tag="AC16O9_04400"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR6515891.1"
                     /translation="MQRNPQLNARGELQHLLSVEGLPRELLLQILDTAESFAGVTERD
                     IKKVPLLRGKSVFNLFFEGSTRTRTTFEIAAKRLSADVINLNVAVSSTSKGESLLDTV
                     ANLSAMQADIFVVRHGSSGAPFLVARHVKPHEHVINAGDGRHAHPTQGLLDLYTIRHY
                     KRDFSRLVVAIVGDVLHSRVARSLIHGLTTLGVPEVRVIGPQTLIPAGAAALGVRVCH
                     AMKDGLRDADVVVMLRLQNERMSGALLPSAQEFNKHYGLTADKLALARADAIVMHPGP
                     MNRGVEIDSNVADGPHSVILPQVTFGIAVRMAVMSMVAGGG"
     gene            complement(5817..6317)
                     /gene="pyrR"
                     /locus_tag="AC16O9_04405"
     CDS             complement(5817..6317)
                     /gene="pyrR"
                     /locus_tag="AC16O9_04405"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR6515892.1"
                     /translation="MAVLPEAEALCEKLARELRPRVGATSAMIGIHTGGAWVAERLHA
                     ALGLNEPLGLLDIAFYRDDYDRKGLARDPKRSKIPFDVAGKDLLLVDDVLYTGRTVRA
                     AINELFDYGRPATITLVVLVERDGRQLPICAQHAGARLEVPAGQRLRLKRSGEGRLSL
                     EFERAA"
     gene            complement(6301..6693)
                     /gene="ruvX"
                     /locus_tag="AC16O9_04410"
     CDS             complement(6301..6693)
                     /gene="ruvX"
                     /locus_tag="AC16O9_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6515893.1"
                     /translation="MPEARTVLAFDFGTRRIGVAVGEAELGTAHPLAAIAAPGFAAID
                     KLVKEWRPDALVVGLPVAERGEHRLAKRVQRFARQLAGRFRLPVETVDERYTSVEAES
                     RLRGAKRKPVDSVAAQLILEQYFHGRAA"
     gene            complement(6686..7207)
                     /locus_tag="AC16O9_04415"
     CDS             complement(6686..7207)
                     /locus_tag="AC16O9_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6515894.1"
                     /translation="MPNMADPYFAKSLTYVCEHNDQGALGVVVNRPIDMTLQALFERL
                     SLTLREKTLSDAPIYFGGPVQTDRGFVLHEPAGNWQSTLKVRDAIGLTTSKDILEAVG
                     RGEGPDKLIVTLGYAGWSAGQLEHEISQNAWLTVEAKDAIIFDLPSEERLPAAMELLG
                     VDYARLADSAGHA"
     gene            7268..8623
                     /locus_tag="AC16O9_04420"
     CDS             7268..8623
                     /locus_tag="AC16O9_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR6515895.1"
                     /translation="MTLDSAALAWFRRDLRSFDHAALHAALRAHGRVHCAFVFDTEIL
                     DALDARADRRVDFIWRSVAELRQALAGLGGGLHVLHGRAREEIPRLAVRLGAAAVYAS
                     RDYEPQAIDRDREVAERLAAAGIAFHTRKDQVIFELDEVLTGGGTPYSVFTPYKNAWL
                     KKLDDFCVKPYPIARHASRLAKSGSELPALPALGFEPAPMALPAGASGGKRLFAEFRR
                     RIDDYAKKRDYPALKGPSYLSVHLRFGTVSIRELARFAWKRKGAGAATWLSELCWRDF
                     YFAVLAARPDVVDHAFKREFDALRWDDDETLWRAWCEGRTGYPLVDAAMRCLNQTGTL
                     HNRLRMVSASFLVKDLGIDWRRGERYFAEKLNDYDLAANTGGWQWAASTGCDAQPWFR
                     IFNPVTQSRRFDPQGAFIRKYVPELARLDARAIHEPRDPVVDHAIARKRTLQRYGAVR
                     Q"
     gene            complement(8617..10434)
                     /locus_tag="AC16O9_04425"
     CDS             complement(8617..10434)
                     /locus_tag="AC16O9_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025055.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HIR6515896.1"
                     /translation="MDLKALRARTAALRGAALRGVAVLPLRRQLALLAALLVMLLAGA
                     LWLAWSEYRDAHFGEGHLAAAAEMRMLSQRLAKAALRGLHGDADAFPQIQASRDGFAA
                     ALARLTRDARAPDALEDLRMDWARTEKNATLLLRDQKLLVALARTAERLERANPQLLE
                     RAERVQNLKLAQGALPAEVAAASRLVMLTQRLARGAQILVAAERVAPEIEFLLGKDVN
                     EFRGTLARLRGGARDEDLVKALEDLGAAFGPLQDAMVAVLGTRERLAGAKDAAFAIHA
                     DSERLLGAVDRVVSAYRAAPSRVVNEAGLAGTALALIAALLLLALLYVIDERRGRAAA
                     ARRLEVMRRHHERDLEAVQQLRRDLESLTAGDLTARATVGSEITYPAAAALNDALDGL
                     RALVARIAHAAARVGEAAGSAQSAADGLLESARLQSARIKGASARALGMARAVTDLSA
                     RSNQSAELAQASLAAAVQGRQAVQAALSVVGDMGRRVRVAAASVDKVRGASPEMARTA
                     NQLHQVAAQAEALGASASGRTLADLGETLRALADDIGRVSKTAKSQGAAASAIAGGVR
                     EMLAGAEQAAQAATRAAAAVEEVAALLRELQDALQNLRY"
     gene            complement(10451..10636)
                     /locus_tag="AC16O9_04430"
     CDS             complement(10451..10636)
                     /locus_tag="AC16O9_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR6515897.1"
                     /translation="MTENQVQYKTWMCLICGYVYDEAAGSPDEGIAPGTRWEDVPINW
                     TCPECGARKEDFEMVEI"
     gene            10714..11472
                     /gene="thiD"
                     /locus_tag="AC16O9_04435"
     CDS             10714..11472
                     /gene="thiD"
                     /locus_tag="AC16O9_04435"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042724 - thiamine-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR6515898.1"
                     /translation="MLAAPPNVLAIAAADPTGGAGLQADILTLASLGCHPLSVLTAYT
                     VQDTHGVTALHAVDPAHVTQQADCVLADAPVRAIKIGVVASAANARAIAGILARHPQV
                     PVVLDPVLASARGDALGDEGAMRALCELLLPRATVATPNSVEARALGGAAAMLARGCR
                     FVLVTGTHEPGDDVVNVLHGAAGVLREDRWPRLPGSFHGSGCTLASAIAARLAHGDAV
                     GEAVHRAQDYTWCALERGYRPGAGQHVPDRLHAR"
     gene            11469..12068
                     /gene="thiE"
                     /locus_tag="AC16O9_04440"
     CDS             11469..12068
                     /gene="thiE"
                     /locus_tag="AC16O9_04440"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR6515899.1"
                     /translation="MTRLRGLYAITPADLSRVEAALASGALCALQYRAKGTEAARRLS
                     EARHLAQLCRAHGVPLIVNDDVELALVVDAAGAHLGRDDGDITAARARLRGRLLGASC
                     YDRPELARRAVAAGADYVAFGSVSDSPTKPAAVRAPLELFALDLGVPKVAIGGITLDN
                     APRVLAAGADAIAVISDLFDAPDIAARATQYGRLFDERR"
     gene            12055..13338
                     /gene="hemL"
                     /locus_tag="AC16O9_04445"
     CDS             12055..13338
                     /gene="hemL"
                     /locus_tag="AC16O9_04445"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR6515900.1"
                     /translation="MKDANQTLFARAQACIPAGVNSPVRAFRAVGGTPPFFERASGAH
                     LWDAEGRRYIDYVGSWGPMVAGHTHARVVEAVQAAASRALSFGAPTAAEVEMAEMLCR
                     LVPSLELVRLVSSGTEATMTAIRLARGFTGRSLIVKFEGCYHGHADALLVKAGSGALT
                     FGNPSSAGVPAETAAHTVVLDYNDAAQVKALFEKRGAEIAGVIVEPVAGNMNLVLPAP
                     GFLETLREQCTRHGAVLILDEVMTGFRVALGGAQARFGIRPDLTTLGKVIGGGLPVAA
                     VGGRRDIMERIAPLGPVYQAGTLSGNPVAVAAGLATLKLVDTPDFQPRIEATTRRLVE
                     GLAAEAKRAGVTFSSQSVGSMFGIYFRAMPPKSFAEVMQCDAQRFNRFFQSMLAQGVY
                     LAPSAYEAGFVSSAHDEKDIEQTLAAAQEAFRHAN"
     gene            13372..13965
                     /locus_tag="AC16O9_04450"
     CDS             13372..13965
                     /locus_tag="AC16O9_04450"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6515901.1"
                     /translation="MRIVTRELPRLERAKFTTHLRALEHEDRRLRFGVVIPDAAIDDY
                     VARIDFARDTVFGVFDDALQLAGAAHLARADAHAELGVSVLPGHRGRGIGAALLARAH
                     VHARNQGIDVLFMHCLAENGAILHLARKQNMRIVAEHGEADARLELPPANPASIAQAL
                     FDERVGLFDYALKSQFDTARRLILAGVLTVPPLPRRT"
     gene            complement(13979..14689)
                     /gene="mtgA"
                     /locus_tag="AC16O9_04455"
     CDS             complement(13979..14689)
                     /gene="mtgA"
                     /locus_tag="AC16O9_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR6515902.1"
                     /translation="MRLRKLRRAWRVAGATLAVLAVVVLLVQAWFYAQILWWSHSAPT
                     STAFMERRLDELRAKDPRARLQQRWAPYARISVELKRAVIAAEDAKFLDHEGFDWEAI
                     QKAMEKNERRGRVVAGASTISQQLAKNLFLSGARSWLRKAQEAVITAMLELAMDKRRI
                     LELYLNFAEWGDGVFGAEAAARHHFGVSAAQLDAAQAAWLAAILPSPRRYARGASTLY
                     LDTRIATIRRRMEYAQIP"
     gene            complement(14693..15493)
                     /gene="aroE"
                     /locus_tag="AC16O9_04460"
     CDS             complement(14693..15493)
                     /gene="aroE"
                     /locus_tag="AC16O9_04460"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6515903.1"
                     /translation="MPDRYAVIGHPVAHSKSPWIHAQFARATREDIAYAAIDAPPAGF
                     VAAVENFRAGGGKGLNVTLPFKEQAFRYCKGTSARAAAAQAVNTLVLDRAQPFGDNTD
                     GVGLVRDLTANLGRSLAGAQVLLLGAGGAAQGVLAPLAEAGAAAIVIANRTAARAEAL
                     AARVPLATACGFDALEGRAFDLVVNATSAGLAGELPRLPRGLFRPGALAYDMVYGRET
                     EFLAMARAAGAATSDGLGMLVEQAAESFLVWRGVRPDTRAVLAALRAR"
     gene            complement(15498..16322)
                     /locus_tag="AC16O9_04465"
     CDS             complement(15498..16322)
                     /locus_tag="AC16O9_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6515904.1"
                     /translation="MAASLLLHAILLSTHFKFPDQLRWKSGEQALEVILVNARTQQRP
                     ARSDALAQANLDGGGDTDQARRARSPLPVIDPRRPGRDLAQAERRVRELEAQQRKLLA
                     QSRRTAPAVAASGERHAPAEAPAPRHSGRDHEDLSLMAMRLQAEINRNLDAYQKRPRV
                     KHIGASAVEYRFAQYEEDWRLKVERVGTVNYPARARGKLYGDLQLTVTLKPDGNVHSV
                     ELDRSSGLKVLDDAAFRIVRMASPYAVFPADIRRDTDLLAITRTWFFGRGDKFWTE"
     gene            complement(16449..18332)
                     /locus_tag="AC16O9_04470"
     CDS             complement(16449..18332)
                     /locus_tag="AC16O9_04470"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HIR6515905.1"
                     /translation="MHVLYEEDGEFKVGTVLAQAPASYQVESPHGRRSKVKAAAVLLR
                     FERPGGAELLEAARDYAAGMDTDFLWQCCGAGEFGFEALAREYVGREPSPAEAAGVLM
                     KLHSAPMYFHRRGRGRYQAAPAETLKLALASAEKKQRVQAQIADWAASLARFACPPEV
                     EQLRDELLYAPDRNKPETKALEQACQATGLTSARLFERCGLIADTHAYHLNRFLFEFY
                     PRGTAFPPHEVPAPPAELPLAAVQAFSLDDATTTEIDDAFSLTRVSDDEWRVGIHIAA
                     PALGIAPGSALDALARERLSTAYMPGVKFTMLPEDVIVRYSLDAGEERAAVSLYVTVS
                     AADLAVRGHHSRMERVRVCANLRHGEIAALDAAFEAGTRAGLPHEDALRTLWRFAEAL
                     ERRRGKPSVNAAVLDYVFRVEDGRVRIEPRRRGAPLDKLVSELMILANASWGELLAER
                     DVAALYRVQSSGKVRMSVHAEAHEGLGLACYAWLSSPLRRYVDLVNQWQLVAALGARK
                     PPFARTSEVLLAALRAFEVTHARYDEYQRAMENYWCLRWLLQESVTEARAVVVRENLV
                     RFEDLPLALRVPSLPALAAGTRVRLAIESVDLLERSLACAYRETLQPGGGEAPPLDER
                     EER"
     gene            complement(18333..18887)
                     /locus_tag="AC16O9_04475"
     CDS             complement(18333..18887)
                     /locus_tag="AC16O9_04475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HIR6515906.1"
                     /translation="MIIYLHGFNSSPQSHKAQVFARAMAERGLGARYACPALPPRASD
                     AIAEIERLRPDGGTCFVGSSLGGYYATYLAEKHEARAVLLNPAIVPHLGLRAYLGEQK
                     NLHTGEPYQLTEAHLREWQALWVPRVTPSRYLLIVETGDEVLDYRQAVARYAGAGQVV
                     IEGGDHSLQSFPRHLPRILTFAGL"
     gene            complement(18884..20248)
                     /gene="mpl"
                     /locus_tag="AC16O9_04480"
     CDS             complement(18884..20248)
                     /gene="mpl"
                     /locus_tag="AC16O9_04480"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR6515907.1"
                     /translation="MAAHLHILGICGTFMGGLAAIARQAGLRVTGCDANVYPPMSEQL
                     AALGIELAEGYDAGQLARAPDLWVIGNVVTRGNPLMEAILDAGQRYVSGPQWLAEHVL
                     AGRHVLAVAGTHGKTGTASMLAWILEHAGRRPGFLIGGVPRNFGVSARLGESGLFVIE
                     ADEYDTAFFDKRSKFVHYSARTAILNNLEFDHADIFDDLAAIERQFHHFVRILPHSGL
                     IIANSRDEAVERVLARGRWTPVERFGVDGGWQAGAPDAENGFEVRWEGRALGRVRWSQ
                     AGEHNRLNALAAIAAARHAGVEPQAAIEALGTFKGVRRRLELRGTVRGVAVYDDFAHH
                     PTAIRATLMGLRALGGAGRILAVLEPRSNTMKLGVMKDRLAASLEPADRAYVYAAGLG
                     WDPAAALQRLGERARSFEQLEGLVAAVAAEARAGDRVLVMSNGGFGGVHGKLLAALGA
                     QATA"
     gene            20317..20925
                     /locus_tag="AC16O9_04485"
     CDS             20317..20925
                     /locus_tag="AC16O9_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515908.1"
                     /translation="MSRSNSRQNGMRARIAAAAARLMAEDGIDDFALAKRKAARQLGA
                     PATEALPANHEIEAELRAYRELYQAEEHPELIAELRRVALDAMRALAHFTPYLTGPVL
                     TGLAGPYAEIELQLFPESTKDVELFLLERNLAFDTAEGRRFAGDRARAVSVFKLDWQG
                     APLKLQVFDPRDERLALKTSQAGRVVERAGIAEVGQLVGKSS"
     gene            21078..21536
                     /gene="aroQ"
                     /locus_tag="AC16O9_04490"
     CDS             21078..21536
                     /gene="aroQ"
                     /locus_tag="AC16O9_04490"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR6515909.1"
                     /translation="MKSRSVLLLNGPNLNLLGTREPHLYGRDTLAQIERRLAARAKAA
                     GVGLACLQTNHEGIAVDRVQQAQRERVGFIILNPAAFTYSSIALRDALTAVQIPFIEV
                     HLSNIHAREPWRAHSHFTAVAVGSILGLGSRGYDLALEYAIERLKGGAGA"
     gene            21587..>21753
                     /locus_tag="AC16O9_04495"
     CDS             21587..>21753
                     /locus_tag="AC16O9_04495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR6515910.1"
                     /translation="MDLRKLKKLIDLVQESGIAELEITEGEEKVKIVKGGEATVTPLA
                     PAAPAPGAAAPA"
BASE COUNT         3156 a         7836 c         7565 g         3196 t
ORIGIN      
        1 cacaccgtgt gcagcttttg ctcgcgcagg attcccttga tctcggtgaa gcgcgagccg
       61 agcgccggcg cccgcacgcg gatccacgcc ggcttgggca gcgccggctt ggacacgatc
      121 ttcaccggga tgcgcgcggt cttggcccgg cccttctcct tgacgcccgc ctcacgcatg
      181 ctcgagcctt tgcgcgatca gtcgcgcgag gcggatgccg acctccttcg ccgtgcccag
      241 caccccgaag tcgcgtgcct gcaccacgct gaggcccggg tagccgcacg ggtcgatggc
      301 ctggaacggt gtgagatcca tgtcgacgtt cagcgcgatg ccgtgccagg cgcggccgcg
      361 cgtgacgcgg atgccgagcg aggcgatctt cgcgccgccg acgtacacgc ccggcgcgcc
      421 ggccttcctt tcggcctgca cctcgtggcg cacgagaaag ttgatcacgc actgctcgag
      481 cagatgcacg aaagccttca ctttgatacc gcggcgtgca aggtcgacca gcgtgtacac
      541 gatcgcctgg cccgggccgt ggtaggtgat ctcgccgccg cgctcggact gcaccaccgg
      601 gatgccgttc gcctgcttcg ggccgaaggc cgggtcggcg ccctggccga gcgtgtacac
      661 cggcgggtgc tccacgatcc aaagctcgtc cggcgtgtcg gcgtcgcgct gcagggtgaa
      721 ctcgcgcatg cgcgccgcca cggccccgta ctcggcgagg ccgaggctct tcaggcgcgt
      781 cgcgagcgcg gcttccatct cagagcacca tgaccaccga tggatggtcg caaaggtcgc
      841 ggtacagctc gtcgagctgc tcgcgcgagg tggcgcggat cgtgcaggtg acgctgagat
      901 aggttccttg gcggctcggg cgcagttcca tggtggcggg atcgaagtcc ggtgcatgct
      961 tcctgacgat gccgaggacg gtcgcggcga agcgcttttc cttgcgtccc atgaccttga
     1021 tcgggaagtc ggtgggatag gaaagcagcg tttcttcgtt ctgaatcggc atcaggccga
     1081 gacggcctcc ttccgcatca cgcgacgctt gaattcctga tagccctccc aggcgcggcg
     1141 aaacagcgga cccggcctgc cctcgcccac cggcttgccg tcgagctcga cgactgccag
     1201 gatctccttc gacgacgagg tgacccagag ctcgtccgcg gcgcgcacct cggactcgcg
     1261 gatgtcgcgg aactccagcg ccagcccgag ctgctgcgcg atctccacca ccacgtcgta
     1321 ggtgatgccg ggcaggatca ggttggactt gggaggagac agcacgaccc cgttcttcgc
     1381 cacgaacacg ttggaggccg aggcctcggt caggatgccg tcgcgaaaca ggatcgtctc
     1441 cgccgcgcct gcgtcggccg aagcctggcg caacagaacg ttgccgatga gcgagatcga
     1501 cttgatgtcg cagcgcagcc accggttgtc cggcgcgctc accgccgcga cgcccttctc
     1561 gaccagctcc tgcggcggat tcaccagcgg gttggacatc gcgaacaccg tcggctcgac
     1621 gccggcgggg aacgcgtgat cgcgcttggc cacgccgcgc gtcacctgca ggtacacacc
     1681 ctggtcttcc cacggctgct tggccaccag ctcgaggatg atcgagcgcc acttgtcgcg
     1741 cgcgtaggga ttcgcgatgc gcacggcggc gaggctgcgc tccaggcgcg cgaggtgctc
     1801 gtccatgcgg aaaggggcgc gcgagtacac cgggatcagc tcgtagacgc catcgccgaa
     1861 gatgaagccg cggtcgagca ccggcaccgt cgcctgctcg agcggcagga acctgccgtt
     1921 caggaagacc atctgttgcg ccccgctact tgagccagag cctcagtgta tcccaggcgc
     1981 ggccgaggat tcccgcctgc ggcacgcttt ccagcgccac caccggatac tcgccgagcg
     2041 gccggccgtc gagcgccacg cgcaggacgc ccaccttgtc gcctttttgc accggcgcca
     2101 gcaccggcgg gtgcgtgagg agctctgccg caagcagctt cgcttcgccc ttgggcaccg
     2161 tcaccaccag gccacgctcg aagccggcgc gcaccgcttc gtgcgcgcct ttccagacgt
     2221 ggatcgcctt caccggctgc cccgcctcgt acaggcgcac gccgtcgaac ccctggtagc
     2281 cccagttgag cagcttctgc gattcgcgcg cgcgcgcggc ctccgagtcg gcgccaagca
     2341 gcacgctgac cagccgccgg ccgttgcgct ccgacgaggc gatcaggcaa tagcccgcgg
     2401 cctcggtgaa gcccgtcttc acgccgtcca cgctcggatc gagccacagg agccggttgc
     2461 ggttcatctg cgtgatgtcg ttgtggcgga actccttcat cgcgtagtag cgcgcgtact
     2521 gctcgggata gtcgcggatc agcgccgccg cgagcagcgc caggtcgcgc gccgtcgagt
     2581 agtgctgcgg gtgcggcagg ccggtggcgt tgacaaagcg cgtgtcgcgc aggtcgaggc
     2641 gcgccgcttc gcggttcatc tgctcggcga accgcgcttc cgtaccggcc agcgcctcgg
     2701 cgagggcgat gcaggcgtcg ttgcccgact gcaccaccat gccgcgcacc agcgtatcca
     2761 cgctcacgct gctgccttcc tcgaggaagg tgcgcgaccc cggcgcctgg cgtgcgtgcg
     2821 ccgatacctg cacctgctgc tcgggcgtga ggcggccctc gcgcagcgcg ccgaacacga
     2881 gatacgcggt catcagcttg gtgagcgagg cgggctcgac gcgctcgtcg gccttgtccg
     2941 cggccagcac ctggccactg gtcaggtcca ggacctggaa agcgcgcgcc gcgaccgcgg
     3001 gcggctgcgg cgcctgcgcg ctcgcgcccc aggcaacgaa aagaaacgcc gacgcgatca
     3061 aggctgcgcg cataaccgtt acctttgcga tgtaagccgc gcgccgcccg ggacgatctg
     3121 ctcgaccagc acgcgcgcgc tgcccgcggc cacgtagccg agcttgtagg ccgcggcgta
     3181 agacaggtcg atgacgcggt tggagtggaa cgggccgcgg tcgttgacgc gcacggtcac
     3241 cgtgcggccg ttggcgaggt tggtgacgcg cgcgtagctc gggatcggca ggatcggatg
     3301 cgcggcgctc atcttgtaca tgtcgtaggg ctcgccgctc gaggtcgcgt tgccgtggaa
     3361 gcgcttgccg taccaggtgg cgacgccgcg ctgctggaac ggcccgacgc gcgtgagcgg
     3421 cacgtagcgc ttgccgaagc gctcgtaagg ccggttggcc cagcggtgca acggctcttc
     3481 gcgtggcacc gcgtcgcgga tggatgccag gtccggcacc cgcgcgcccg ggccgtcgtc
     3541 cttgtagtag ccgccggggc gatcgggcgt cgagccgcag cccgcgagca gcgccgcgag
     3601 gagcaccagg cgcctcatgc ctcgtacacc acctgaccgt cgacgagggt ggcgcgcacg
     3661 cgcccggcca gctcgagtcc gaggaacggc gtgttcttgc cctggctctt cagcgccgac
     3721 gcgctcaccc ggaacggcgc ctcgggatcg aacacgatga ggtcggcggg cgccccgggc
     3781 gcgatgcgcc cggcatcgac gccgaggatg cgcgccggcg cgatggtcac gcgggcgagg
     3841 gtctgcgcca agttgagttt cgcctgcact ccccacttga gcgcgagcgg cagcaaaagt
     3901 tccagcccgc tcgctccagg ctccgcctcg gcgaagggca ggtgcttgcg gtcctcgtcc
     3961 accggcgtgt gatcggaaac gatgcaatcg atcgttccgt cggcgagccc ggcgccgagc
     4021 gcgtcgcggt cgcggctgct gcgcagcggc ggctcgagcc ggcactgcgc gtcgaagtag
     4081 ccgaggtcca tgtcggacag atgcacgtgg tgcacgccga cgtcgcaggt aagcggcagg
     4141 ccatcgtggc gcgcacgccg cagcatggcg acaccggccg cgctcgagat gcggcacaca
     4201 tgcacgcgcg cgcccgtggc gcgcaccagc tcgagcagcg ttgccagcgc cacggtctcg
     4261 gcgaacgccg gaatgcccgc gagtccgagg cgcgtggcga tctcgccgtc gtgcgccacg
     4321 ccgccctgcg ccagccacaa gtcctcggcg cgcagccaca ccgggaagcc gaacgtcgcc
     4381 gcgtactgca gcgcgcgcca cagcacctgc gtgtccgcaa tcgcggcgtc ggcctgcgag
     4441 aaggcgacgc agcccgcctc ggcgagctct gccatttcgg tgagctggcg gccctcgagc
     4501 ttcacggtga gcgcgcccac cgggtagacg cgggcgcgcg ccagcgcctt ggcgcgccgg
     4561 cgcagcatgt cgacgaggcc cggctcgtcg agcggcgggt cggtatcggg cgggcaggcg
     4621 aggctggtga cgccgccggc caccgcggcg agcatctcgg attcgagcgt cgctttgtac
     4681 tcgtagcccg gctcgcgcaa ccgcgctgcg agatcgatga gtcccggcgc gacgaccagc
     4741 ccctgggcgt cgatgacccg atcaggcttg ccgccgggcg ccgccacgat cttgccgtcg
     4801 gcgatcgaca cgtcgcccac ggcgtcgcgg ccggtcgccg ggtcgaccac gcgccccccc
     4861 ttcacgagca gcttcatccg ccgcctgcca ccatggacat caccgccatg cgcaccgcga
     4921 taccgaaggt gacctgcggc aggatcaccg aatgcggacc gtcggcgacg ttggaatcga
     4981 tctccacgcc gcggttcatc ggtccggggt gcatgacgat ggcatcggca cgcgccaacg
     5041 caagcttgtc ggcggtgagc ccgtagtgct tgttgaactc ctgggccgag gggagcagcg
     5101 ccccgctcat cctctcgttc tgcagccgca gcatcaccac cacgtccgcg tccctcaggc
     5161 cgtccttcat cgcgtggcag acgcgcacgc cgagcgccgc ggcgccggcg gggatcagcg
     5221 tctgcggccc gatgacgcgc acttcgggca cgccgagcgt ggtgaggccg tggatcaatg
     5281 agcgcgcgac gcgcgaatgc agcacgtcgc cgacgatcgc caccacgagg cgcgagaaat
     5341 cgcgcttgta gtggcggatg gtgtacaggt ccagcagccc ctgcgtcgga tgcgcgtgac
     5401 ggccgtcgcc ggcgttgatg acgtgctcgt gcggcttcac gtgtcgcgcg acgaggaacg
     5461 gcgcgccgga agagccgtgg cgcacgacga agatatcggc ctgcatcgcc gagaggttgg
     5521 cgaccgtgtc gagcagcgac tcgcccttgg aggtcgagct cacggcgacg ttcaggttga
     5581 tcacgtcggc cgagaggcgc ttggcggcaa tctcgaacgt ggtgcgcgtg cgcgtcgagc
     5641 cttcgaagaa caggttgaac acgctttttc cccgcagcag cggcaccttc ttgatgtcgc
     5701 gctcggtcac accggcgaac gattcggcgg tgtccaggat ctgcagcagc agctcgcgcg
     5761 gcaggccttc gaccgacagc aggtgctgca gctcgcccct cgcgttcagc tgcggattac
     5821 gctgcacgct cgaactccag cgacagccgc ccctcgccgc tgcgcttcag gcgcagccgc
     5881 tggccggcgg gcacctcgag gcgcgcgccg gcatgctgcg cgcagatcgg cagctggcgc
     5941 ccgtcgcgct cgaccagaac cacgagcgtg atggtggccg gccggccgta gtcgaacagc
     6001 tcgttgatcg ccgcgcgcac cgtgcgaccc gtatagagca cgtcgtccac cagcagcaag
     6061 tccttgccgg cgacgtcgaa cgggatcttg gagcgcttcg ggtcgcgcgc aagccctttg
     6121 cggtcgtagt cgtcgcggta gaaagcgatg tcgaggagcc cgaggggctc gttcagcccg
     6181 agcgccgcgt gcagccgctc ggcgacccag gcaccgccgg tatgaatgcc gatcatggcg
     6241 ctcgtcgcgc ccacgcgcgg ccgcagctca cgcgcgagct tctcgcagag cgcctcggcc
     6301 tcaggcagca cggccatgga agtactgctc gagaatgagc tgcgccgcca ccgaatcgac
     6361 cggtttgcgc ttcgcgccgc gcaggcggct ctcggcctcc accgacgtat agcgctcgtc
     6421 caccgtttcc accggcaggc ggaatcgccc cgcgagctgg cgtgcgaagc gctgcacgcg
     6481 cttggcaagc ctgtgctcgc cgcgctcggc taccggcagg ccgaccacca gggcgtcggg
     6541 gcgccattcc ttcaccagct tgtcgatcgc ggcgaagccg ggcgcggcga tggcggcgag
     6601 cgggtgcgcg gtgccgagct ccgcttcccc tactgcaacg ccaatgcgcc tggtgccgaa
     6661 atcgaatgcg aggacggtgc gcgcctcagg catgtcccgc cgaatctgcc aggcgcgcgt
     6721 agtccacgcc gagcagctcc atcgccgccg gcagccgctc ttcggagggc aggtcgaaga
     6781 tgatggcgtc cttggcctcg accgtgagcc aggcattctg cgaaatctcg tgctcgagct
     6841 gccccgccga ccagccggcg tagccgagcg tcacgatgag cttgtcgggc ccttcgcccc
     6901 ggcccaccgc ctccaggatg tccttcgacg tggtcaggcc gatggcgtcg cgcaccttga
     6961 gcgtcgactg ccagtttccg gccggctcgt gcaggacgaa gccgcggtcg gtctgcaccg
     7021 gcccgccgaa atagattggc gcatccgaca gggtcttctc gcgcagggtg agcgacaggc
     7081 gctcgaaaag cgcctgcagc gtcatgtcga tggggcggtt cacgaccacg ccgagcgcgc
     7141 cctggtcgtt atgctcgcag acgtaggtca gggatttcgc aaagtacgga tcggccatgt
     7201 tgggcatggc gatcagaaag tgatgcgtga ggttgatcga ggacacgctc aggccttatt
     7261 ttaacccatg accttggact ccgccgcgct cgcctggttc cggcgcgacc tgcgcagctt
     7321 cgatcacgcc gccctgcacg ccgcgctgcg cgcccacggg agggtgcact gcgcattcgt
     7381 cttcgacacc gagatcctcg acgcccttga cgcgcgcgcc gaccgtcgcg tggacttcat
     7441 ctggaggagc gtcgccgagc tgcgccaagc gctcgccgga ctgggcggcg gcctgcacgt
     7501 gctgcacggg cgcgcgcgcg aggagatccc gcggctcgcc gtgcgcctgg gggcggccgc
     7561 ggtctacgcc agccgcgact acgagccgca ggcgatcgat cgcgaccgcg aggtcgccga
     7621 gcggctcgcc gcggcgggca ttgcgttcca cacgcgcaag gaccaggtga tcttcgagct
     7681 cgacgaggtg ctgaccggcg gcggcacgcc gtattcggtg ttcacgccgt acaagaacgc
     7741 ctggctgaag aagctcgacg atttctgcgt caagccctac ccgatcgcgc ggcacgcgtc
     7801 ccggctggcg aaatcgggca gcgagctgcc tgcgctgccg gcgctcggct tcgagccggc
     7861 gccgatggcg ctgcccgcgg gcgccagcgg cggcaagcgg ctgtttgccg aattccggcg
     7921 ccgcatcgac gactacgcga aaaagcgcga ctaccccgcc ctcaagggcc cgtcgtacct
     7981 gtcggtgcac ctgcgcttcg gcaccgtgtc gattcgcgaa ctcgcgcgct ttgcatggaa
     8041 gcgcaagggt gccggcgcgg ccacctggct gtcggagctc tgctggcgcg acttctactt
     8101 cgccgtgctc gcggcaaggc cggatgtcgt ggaccacgct ttcaagcgcg agttcgacgc
     8161 cctgcgctgg gacgacgacg agacgctctg gcgcgcctgg tgcgaggggc gaaccggcta
     8221 tccgctggtg gatgccgcca tgcgctgcct caaccagacg ggcaccctgc acaaccgcct
     8281 gcgcatggtg agcgcgtcct ttctcgtcaa ggacctcggc atcgactggc gccgcggcga
     8341 gcgctacttc gccgagaagc tgaacgacta cgacctcgcc gccaacaccg gcggctggca
     8401 gtgggcggcc tccaccggct gcgacgcgca gccgtggttt cgcatcttca acccggtaac
     8461 ccagtcgagg cgcttcgacc cacagggcgc gttcatccgc aagtacgtgc cggagctggc
     8521 gcggcttgac gcgcgcgcga tccatgagcc gcgcgatccg gtcgtggatc acgcgatcgc
     8581 gcgaaagcgc acgctccagc gctacggtgc ggtgcgtcag tagcgcaggt tctgcagcgc
     8641 gtcctggagc tcgcgcagca gcgccgccac ctcttccacc gcggccgcgg cgcgcgtggc
     8701 ggcttgcgcg gcctgctcgg cgccggccag catctcgcgc acgccgcccg cgatggcgct
     8761 cgccgccgcg ccctgcgact tggccgtctt cgatacgcgc ccgatgtcat cggcgagcgc
     8821 gcgcagcgtc tcgcccagat ccgcgagcgt gcgccccgag gcgctggcgc caagcgcctc
     8881 ggcctgcgcc gcaacctggt gcagctggtt cgcggtcctc gccatctccg gcgaggcgcc
     8941 gcgcaccttg tccacgctcg ccgccgccac gcgcacgcgc cgccccatgt cgccgacgac
     9001 cgaaagcgcc gcctgcaccg cctgacggcc ctgcacggcc gcggcgagcg aggcctgcgc
     9061 cagctcggcg gactggttgg agcgcgccga gaggtcggtg accgcgcgcg ccataccgag
     9121 cgcgcgcgcg ctcgcgccct tgatgcgcgc tgactgcaga cgcgccgatt cgagcaaccc
     9181 gtcggcggcc gactgcgccg agccggcggc ctcgccgacg cgcgccgcgg cgtgcgcgat
     9241 gcgcgccacc agcgcgcgca gcccgtccag cgcgtcgttc agcgccgcgg ctgccggata
     9301 cgtgatctcg ctgcccacgg tggcacgcgc cgtgagatcg cccgcggtca gcgactcgag
     9361 gtcgcggcgc agctgctgca ccgcttcgag atcgcgctcg tggtggcggc gcatcacctc
     9421 gaggcgccgc gcggctgccg cgcggccgcg gcgctcgtcg atcacgtaga gcagcgcgag
     9481 cagcagcagc gccgcgatga gcgcgagcgc ggtgccggcg aggcccgcct cgttgacgac
     9541 gcgcgagggc gccgcgcgat acgcgctcac gacgcggtcc acggcgccga gcaggcgctc
     9601 ggaatcggcg tggatggcga acgccgcgtc ctttgcgcca gcgaggcgct cgcgcgtgcc
     9661 gagcacggcg accatcgcgt cctgcagcgg gccgaaagcc gcgccgaggt cctcgagcgc
     9721 cttgaccaga tcctcgtcgc gcgcgccgcc gcgcagccgg gcaagcgtgc cgcggaactc
     9781 gttcacgtcc ttgcccagca ggaactcgat ttcgggcgcg acgcgctcgg cggccaccag
     9841 gatctgcgcg ccgcgcgcca ggcgctgcgt cagcatcacg aggcggctcg cggcggcgac
     9901 ctccgcgggc agcgcgccct gcgcgagctt caggttctgc acgcgctcgg cgcgctccag
     9961 cagctgcggg ttggcgcgct cgagacgctc ggcggtgcgt gcgagcgcca ccagcagctt
    10021 ctggtcccgg agcagcagcg tggcgttttt ctcggtgcgc gcccagtcca tgcgcaggtc
    10081 ctcgagcgca tccggcgcgc gcgcgtcgcg cgtcaggcgc gcgagcgcgg cggcgaagcc
    10141 gtcgcggctc gcctggatct gcggaaaggc gtcggcgtcg ccgtgcagac cgcgcagcgc
    10201 cgcctttgcc agccgctgcg acagcatgcg catctccgcc gctgccgcga ggtgcccctc
    10261 gccgaaatgc gcatcgcggt actcggacca cgccagccac agcgcgccgg cgagcagcat
    10321 gaccagcagc gcagcgagca gcgccagctg gcgccgcagc ggcagcaccg cgacgccgcg
    10381 caacgccgcg ccgcgcagcg ccgcggtgcg cgcgcgcagc gccttcaggt ccaagcgcgc
    10441 cggcgcgcgc tcagatctcg accatctcga aatcttcctt gcgcgcgccg cattccgggc
    10501 aggtccagtt gatgggcacg tcttcccagc gcgtgccggg ggcgatgccc tcgtccggcg
    10561 agccggccgc ttcgtcgtac acgtagccgc agatgaggca catccaggtc ttgtattgaa
    10621 cttggttctc ggtcatcagg cggtgcacgc cgtgtgagtt agaatcgtgc ggcatcttag
    10681 cgtaattcgc gaggcggccg cgccgcgccc cccatgcttg ccgcaccgcc gaacgttctc
    10741 gccatcgccg ccgccgatcc gacgggcggc gccggcctgc aggccgacat tctcacgctc
    10801 gcgagccttg gctgccatcc gctttccgtg ctcaccgcgt acacggtgca ggatacccac
    10861 ggcgtcaccg cgttgcacgc ggtggacccc gcacacgtga cgcagcaggc ggattgcgtg
    10921 ctggccgacg cgcccgtgcg cgccatcaag atcggtgtcg tcgcgagcgc cgccaacgcg
    10981 cgggcgatcg ctgggattct cgccaggcat ccgcaggttc ccgtggtgct cgacccggtg
    11041 ctcgcctcgg cgcgcggcga cgcgctcggc gacgaaggcg cgatgcgcgc gctgtgcgag
    11101 ctgctgctgc cgcgcgcaac cgtggcgacc ccgaacagcg tcgaagcgcg cgcgctcggc
    11161 ggcgccgcgg ccatgctcgc gcgcggctgc cggttcgtcc tggtcaccgg cacgcacgaa
    11221 cccggcgacg acgtggtgaa cgtgctgcac ggcgccgcgg gcgtcctgcg cgaagaccgc
    11281 tggccgcgcc tgcccggcag cttccacggc tcgggctgca cgctcgcttc ggcgattgcc
    11341 gcgcgcctcg cgcacggcga cgcggtcggc gaagcggtgc accgcgcgca ggactacacc
    11401 tggtgcgcgc tcgagcgcgg ctaccgtccg ggcgccggcc agcacgtgcc cgaccggctg
    11461 cacgcgcgat gacgcgcctg cgcgggctgt acgccatcac gcccgcggac ctgtcgcggg
    11521 tcgaagcggc gctcgcttcg ggtgccctgt gcgcgctgca gtatcgcgcc aaggggacgg
    11581 aggcggcgcg ccggctgagc gaggcgcggc atctcgcgca gctttgccgc gcgcacggtg
    11641 tgccgctcat cgtcaacgac gacgtcgagc tcgcgctcgt cgtggacgcg gccggcgccc
    11701 acctcggccg cgacgacggc gacatcacgg cggcgcgcgc gcgcttgcgc gggcggctgc
    11761 tgggtgcgtc gtgctacgac cggccggagc tcgcgcgccg tgcagtggct gcaggcgccg
    11821 actacgtggc gttcgggtcg gtgtccgatt cgcccaccaa gcccgcggcg gtgcgcgcgc
    11881 cgctcgaact cttcgcgctc gatctgggcg tgccgaaagt ggcgatcggc ggcataaccc
    11941 tcgacaatgc gccccgggtg ctcgccgcgg gcgccgatgc gatcgcggtg atctcggacc
    12001 ttttcgatgc gccggatatc gccgcgcgcg cgactcaata cgggaggttg ttcgatgaaa
    12061 gacgctaacc agacgctgtt cgcacgcgcg caggcctgca tccccgccgg cgtcaactcg
    12121 ccggtgcgcg cgtttcgcgc cgtgggcggc acgccgccgt tcttcgagcg cgcgagcggc
    12181 gcgcacctct gggacgccga gggccgccgc tacatcgact acgtcggctc ctggggaccg
    12241 atggtcgccg ggcacacgca tgcgcgcgtg gtggaggcgg tgcaggcggc ggcctcgcgc
    12301 gcgctgtcct tcggcgcgcc caccgccgcc gaggtggaga tggcggagat gctgtgccgg
    12361 ctcgtgccct cgctcgagct ggtgcggctg gtgtcctcgg gcacggaggc gacgatgacc
    12421 gcgatccgcc tcgcgcgcgg cttcaccggc cgcagcctga tcgtcaagtt cgagggctgc
    12481 taccacggcc acgccgacgc gctgctcgtc aaggcgggct cgggcgcgct caccttcggc
    12541 aacccgagct cggcgggcgt tcccgccgag accgccgcgc acaccgtggt gctcgactac
    12601 aacgacgccg cgcaggtgaa agcgctgttc gagaagcgcg gcgcggagat cgccggggtc
    12661 atcgtcgagc cggtcgccgg caacatgaac ctcgtgctgc ccgcgccggg cttcctggaa
    12721 acgctgcgcg agcagtgcac gcggcatggc gcggtgctga tcctcgacga ggtaatgacc
    12781 gggtttcgcg tcgcgcttgg cggcgcgcag gcgcgcttcg gcatccggcc cgacctcacc
    12841 acgctcggca aggtgatcgg cggcggcctg ccggtggccg cggtcggcgg gcgccgcgac
    12901 atcatggaga gaatcgcgcc gctcggcccg gtgtaccagg ccggcacgct ctcgggcaac
    12961 ccggtggcgg tggccgcggg cctcgccacg ctcaagctcg tcgatacgcc ggatttccag
    13021 ccgaggatcg aggcgacgac gcgccgcctg gtcgagggcc tcgccgcgga ggcgaagcgc
    13081 gccggcgtta cgttctcctc gcaatccgtg ggcagcatgt tcggcatcta cttccgcgct
    13141 atgccaccca agagcttcgc cgaagtcatg cagtgcgacg ctcagcgctt caaccgcttt
    13201 ttccagtcga tgctggcgca gggcgtttac cttgcgccct cggcctacga ggccgggttc
    13261 gtgtcgtcgg cgcacgacga gaaagatatc gagcagacgc tcgcggcggc tcaggaggcc
    13321 ttccggcacg cgaactgact ccggcacgat gtttgcgagc gacaatcgcg catgcgcatc
    13381 gtgacccgag agctgccccg gctagaacgc gcgaagttca cgacccacct gcgtgcgctc
    13441 gagcacgagg accggcgatt gcgcttcggc gtcgtcatcc cggatgccgc catcgacgac
    13501 tacgtcgcgc gcatcgactt cgcgcgcgac acggtgtttg gcgtgttcga tgacgcgctg
    13561 cagctcgccg gcgccgcaca cttggcgcgg gccgacgcgc atgccgagct cggcgtctcg
    13621 gtcctgcccg ggcatcgcgg ccgcggcatc ggtgcggcgc tgcttgcgcg cgcgcacgtg
    13681 catgcgcgca accagggcat cgacgtgctg ttcatgcact gccttgcgga gaacggcgcc
    13741 atcttgcact tggcgcgcaa gcagaacatg cgcatcgtcg ccgagcacgg cgaggccgac
    13801 gcgcgccttg agctgccgcc ggccaacccg gcgagcatcg cccaggcgct gttcgacgag
    13861 cgcgtgggcc tgttcgatta cgcgctcaag tcgcaattcg acacggcgcg gcggctgatc
    13921 ctcgccggcg tactcaccgt gccaccgctg ccacggcgca cgtagcgtcg gcgccaccct
    13981 acggaatctg cgcgtactcc atgcgccgcc ggatggtggc gatgcgggta tcgagataca
    14041 gcgtgctcgc gccgcgcgcg tagcgccgcg ggctcggcag gatcgccgcc agccacgcgg
    14101 cctgggcggc gtcgagctgc gcggcgctga cgccgaaatg gtggcgcgcc gcggcttcgg
    14161 cgccgaacac gccatcgccc cattcggcga agttcaggta cagctcgagg atgcggcgct
    14221 tgtccatggc gagctcgagc atcgcggtga tcacggcttc ctgcgccttg cgcagccacg
    14281 agcgcgcgcc cgacaggaac aagttcttgg ccagctgctg gctgatggtg gaggcgcctg
    14341 ccaccaccct gccccggcgc tcgttcttct ccatcgcctt ctggatcgcc tcccagtcga
    14401 agccctcatg gtcgaggaac ttggcgtcct cggcggcgat caccgcgcgc ttgagctcca
    14461 ccgagatgcg cgcatagggt gcccagcgct gctgcaggcg cgcgcgcgga tccttcgcgc
    14521 gtagttcgtc cagccggcgc tccatgaacg cggtactcgt gggcgcgctg tgcgaccacc
    14581 acaggatttg cgcgtagaac caggcctgca ccaggagcac gaccacggcg agcaccgcaa
    14641 gcgtggcgcc ggcgacgcgc caggcacggc gcagcttgcg caaccgcatc gcctagcgcg
    14701 cgcgcagcgc ggcaagcacc gcgcgcgtgt ccgggcgcac gccgcgccag acgagaaagg
    14761 attccgccgc ctgctcgacc agcatgccca acccgtcgct cgtcgccgcg cccgccgcgc
    14821 gcgccatggc gagaaactcg gtttcgcgtc cgtacaccat gtcgtaggcg agcgcgccgg
    14881 gtcggaacag tccgcgcggc aggcgcggca gctcgccggc gagcccggcg gaagtcgcgt
    14941 tcaccaccag gtcaaaggcg cggccctcga gcgcatcgaa gccgcaggcc gtggcgagcg
    15001 gcacgcgtgc cgcgagcgct tcggcgcgcg cggcggtgcg attggcgatg acgatcgccg
    15061 ccgcgcccgc ctccgcgagc ggggcgagca cgccctgcgc agcgccgccc gcgccgagca
    15121 gcagcacctg cgcgccggcg aggctgcggc cgaggttggc cgtcagatcg cgcaccagac
    15181 cgacgccatc ggtgttgtcg ccgaacggct gcgcgcgatc gagcacgagc gtgttgaccg
    15241 cctgcgccgc cgccgcgcgc gcgctggtgc ccttgcaata gcgaaaggcc tgctccttga
    15301 acggcaacgt gacgttcagg cccttgccgc cgccggcgcg gaaattctcc accgctgcga
    15361 cgaagcccgc gggaggtgcg tcgatggcag cgtaggcgat gtcctcgcgc gtggcgcgcg
    15421 cgaactgggc gtggatccag ggggacttgg aatgggcgac cgggtggccg atcaccgcat
    15481 agcggtcggg cacgggctca ttcagtccag aatttgtcgc cgcggccgaa gaaccaggta
    15541 cgcgtgatcg ccagcaggtc ggtgtcacgc cggatgtccg ccgggaacac cgcatagggc
    15601 gaggccatgc gcacgatgcg gaacgcggcg tcatccagca ccttcaggcc cgaggagcga
    15661 tccagctcga ccgagtgcac gttgccgtcg ggcttcagcg taacggtgag ctgcaagtcg
    15721 ccgtagagct tgccgcgcgc cctggccgga tagttcaccg ttccgacgcg ctcgaccttg
    15781 aggcgccagt cctcttcgta ctgcgcgaag cggtactcca ccgcgctcgc gccgatgtgc
    15841 ttcacgcggg ggcgcttctg gtaggcatcg aggttgcgat tgatctcggc ctgcaggcgc
    15901 atcgccatga gcgacaggtc ctcgtggtcg cgcccgctgt gtcgcggcgc cggcgcttcg
    15961 gcgggcgcat ggcgctcgcc cgacgccgcc accgccggtg cggtgcgacg cgactgcgcg
    16021 agaagcttcc tctgctgcgc ctcgagctcg cgcacgcgcc gctcggcctg cgccaggtcg
    16081 cggccgggcc ggcgcgggtc gatcacgggc agcggcgacc tggcgcgccg cgcctgatcg
    16141 gtgtcgccgc cgccgtcgag gttcgcctgc gccagcgcgt cgctgcgcgc cgggcgctgc
    16201 tgcgtgcggg cgttgaccag gatcacttcg agcgcctgct cgccgctctt ccagcgcagc
    16261 tggtcgggaa acttgaagtg cgtcgagagc aggatcgcat gcaggaggag cgacgccgcc
    16321 accgacgcgt acaggacgcg ggaccggtgg tcgagcccgg accagatttc ggccgcgccc
    16381 ggaatgcttg tcgctgcggc tccctgcaat ggcacctcga ggcgataaac cgccgccatt
    16441 ctagggcttt agcgctcttc ccgctcgtcg agcggcggcg cctcgccgcc gcccggctgc
    16501 agggtttcgc gataggcgca ggcgaggctg cgctccagca gatccacgga ctcgatcgcc
    16561 aggcgcaccc gggtgccggc ggcaagtgcc ggcagggacg gcacgcgcag cgccagcggc
    16621 aggtcctcga agcgcaccag gttctcgcgt accaccaccg cgcgcgcctc ggtgaccgat
    16681 tcctgcagca gccagcgcag acaccagtag ttctccatcg cgcgctggta ctcgtcgtag
    16741 cgcgcgtgcg tcacctcgaa agcgcgcagc gccgcgagca gcacctccga ggtacgtgcg
    16801 aacggcggct tgcgcgcgcc gagcgcggcc accagctgcc attggttcac gaggtcgaca
    16861 tagcggcgca gcggcgagct cagccacgcg tagcacgcca ggccgaggcc ctcgtgcgcc
    16921 tcggcgtgca cgctcatacg caccttgccc gaggactgca cgcggtacag cgccgcgacg
    16981 tcgcgctcgg cgagcagctc gccccagctc gcattggcga ggatcatcag ttccgagacc
    17041 agcttgtcga gcggcgcgcc gcggcgccgc ggctcgatcc tcacacggcc gtcctcgacg
    17101 cgaaagacgt agtcgagcac cgcggcgttg acgctgggct tgccgcggcg ccgctccagc
    17161 gcctcggcga agcgccacag cgtgcgcagc gcgtcttcgt gcggcagccc ggcccgcgtg
    17221 cccgcctcga atgcggcgtc cagggcggcg atctcgccgt ggcgcaggtt ggcgcaaacc
    17281 cgcacgcgct ccatgcgcga gtggtggccg cgcaccgcca gatccgcggc cgatactgtc
    17341 acgtacagcg acaccgccgc gcgctcctcg cccgcatcga gcgagtagcg cacgatcacg
    17401 tcctcgggaa gcatggtgaa cttgaccccg ggcatgtagg cggtggacag gcgctcgcgc
    17461 gccagtgcgt cgagcgcgga ccccggcgcg atgccgagcg ccggcgccgc gatgtggatg
    17521 ccgacgcgcc attcgtcgtc cgagacgcgc gtcagcgaga acgcgtcatc gatttccgtg
    17581 gtggtggcat cgtccaggct gaacgcctgc accgccgcga gcggcagctc cgccggcggc
    17641 gccggcacct cgtggggcgg aaacgccgtg ccgcgcgggt agaactcgaa caagaaccgg
    17701 ttcaggtggt aggcgtgcgt gtcggcgatg agcccgcagc gctcgaacag gcgcgcgctc
    17761 gtcagcccgg tcgcctggca agcctgctcg agcgccttgg tctcgggctt gttgcgatcc
    17821 ggcgcgtaca gcagctcgtc gcgcagctgc tcgacctcgg gcgggcaggc aaagcgcgcg
    17881 aggctggcgg cccagtcggc gatctgcgcc tgcacgcgct gcttcttctc ggccgaagcc
    17941 agggcgagct tcagcgtttc tgccggcgcc gcctggtagc ggccgcgacc gcgccggtga
    18001 aaatacatgg gcgccgagtg cagcttcatg agcactccgg ccgcctccgc cgggctcggc
    18061 tcgcggccga cgtactcgcg cgccagcgcc tcgaagccga actcgccggc gccgcagcac
    18121 tgccacagga agtcggtgtc catccccgcg gcgtagtcgc gcgccgcctc gagcagctcg
    18181 gcgccgccgg gccgctcgaa gcgcagcagc acggcggccg ccttcacctt gctgcgcctg
    18241 ccgtgcgggc tttccacctg gtaggaggct ggcgcctgcg caagcacggt tccgaccttg
    18301 aactcgccgt cctcttcgta gagcacgtgc atctacagcc ctgcaaaggt gaggatgcgc
    18361 ggcaggtgcc gcgggaagct ctgcaggctg tgatcgccgc cctcgatcac cacctgccct
    18421 gcgcccgcgt agcgcgccac ggcctggcgg tagtcgagca cctcgtcgcc ggtctcgacg
    18481 atcagcaggt agcgcgacgg cgtgacccgc ggtacccaca gcgcctgcca ctcgcgcagg
    18541 tgcgcttcgg tcagctggta gggctcgccg gtgtggagat tcttctgctc gccgaggtag
    18601 gcgcgcagcc cgaggtgggg cacgatcgcc gggttgagca gcaccgcgcg cgcctcgtgc
    18661 ttttccgcca ggtaggtggc gtagtagccg ccgagagagg acccgacgaa gcaggtcccg
    18721 ccgtccgggc gcagccgctc gatctcggcg atcgcgtccg aggcgcgggg cggcagcgcc
    18781 gggcaggcgt agcgcgcgcc caggccgcgc tccgccatgg cgcgcgcgaa gacctgcgcc
    18841 ttgtgagact gaggcgagga gttgaagccg tgcaggtaga tgatcacgcc gtggcctgcg
    18901 cacctagagc ggccagcagc ttgccgtgca cgccgccaaa gcccccgttg gacatcacca
    18961 gcacccgatc ccctgcgcgc gcctcggcgg cgaccgccgc caccaggccc tcgagctgct
    19021 cgaagctgcg cgcgcgctcg cccagccgct gcaaggccgc tgcggggtcc cagcccagac
    19081 ccgccgcgta aacgtaggcg cggtcggcgg gctcgaggct cgccgcgagg cgatccttca
    19141 tgacacccag cttcatcgtg ttggagcgcg gctcgagcac ggcaagaatg cgcccggctc
    19201 cgcccagcgc gcgcagcccc atcagggtgg cgcgaatcgc cgtcgggtga tgggcgaaat
    19261 cgtcgtatac cgcgaccccg cgcaccgtgc cgcggagctc gaggcgccgg cgcaccccct
    19321 tgaacgtccc gagcgcctcg attgccgcct gcggctcgac gccggcgtgg cgcgcggcag
    19381 cgatcgccgc cagcgcattg aggcggttgt gctcgcccgc ctgggaccag cgcacgcggc
    19441 cgagcgccct gccctcccag cgcacctcga agccgttctc ggcgtcgggc gcgccggcct
    19501 gccacccgcc gtccaccccg aagcgctcca ccggcgtcca gcggcctcgg gcaagcactc
    19561 gctctacagc ctcatcccgg ctgttcgcta tgatcagtcc cgaatgcggc aatattctta
    19621 caaaatgatg aaattgtcgc tcgatagccg caagatcgtc gaagatgtct gcatgatcaa
    19681 actccaggtt attcagaatc gcggttcggg cggagtagtg gacgaacttg gagcgcttgt
    19741 cgaagaaggc cgtgtcgtac tcgtccgcct caatgacaaa cagccccgac tccccgagcc
    19801 tggcgctgac gccgaaattg cggggcacgc ccccgatgag gaatccgggc ctgcgcccgg
    19861 cgtgctcgag aatccaggcg agcatcgacg cggttcccgt cttgccgtgc gtccccgcca
    19921 ccgccagcac gtgacgtccg gcgagcacgt gctcagcgag ccactgcggg cccgagacat
    19981 agcgctggcc cgcatcgagg attgcctcca tgagcgggtt gccgcgcgtg accacgttgc
    20041 cgatcaccca caggtcgggg gcgcgcgcga gctgcccggc gtcgtagcct tcggcgagct
    20101 cgatgccgag cgcggcgagc tgctcgctca tcggcgggta cacgttggcg tcgcagccgg
    20161 tcacgcgcag gcccgcctgc cgcgcgattg ccgccaggcc gcccatgaac gtgccgcaaa
    20221 tgcccagaat gtgcaggtgt gcggccatgg cgcaggggaa agggcggcga ttgtacgcga
    20281 tggcccccgg gggcgttgcg ctatgatcgt cccgctatga gccgctcgaa ttcgcggcaa
    20341 aacggcatgc gcgcgcgcat cgccgccgcc gccgcccggc tgatggccga ggacggcatc
    20401 gacgacttcg cgctcgccaa gcgcaaggcg gcgcgccagc tcggggcgcc cgccaccgag
    20461 gcgctgcccg ccaatcacga gatcgaggcc gagctgcgcg cctatcgcga gctgtaccag
    20521 gccgaagagc acccggagct gatcgccgag ctgcgccgtg tcgccctgga cgccatgcgc
    20581 gccctcgcgc atttcacgcc ctatctcacc gggccggtgc tcaccgggct ggccggcccc
    20641 tatgccgaga tcgagctgca actgtttccg gaaagcacca aggatgtgga gctgtttctg
    20701 ctcgagcgca acctcgcttt cgataccgcc gaggggcgcc gcttcgccgg cgaccgcgcc
    20761 cgcgcagtca gcgttttcaa gctcgactgg cagggcgcgc ccctcaaatt gcaggtattc
    20821 gacccgcggg acgagcggct ggcgttaaaa acctcacagg ccgggcgcgt cgtagagcgc
    20881 gccgggatcg ccgaagtggg gcaacttgtg ggtaaaagta gctgatcttc gtcgaaaagt
    20941 gtcaatcgtg ggccgaacag gtcttgccct ttgacaactc aggccgcaag cctatactgg
    21001 gtccctagcc gccgagaaga gaaacggcgt aatttcggct atattgccgt cttcttcagc
    21061 gaaaaatggc caaatcaatg aagtcaaggt ccgtactgct tctgaacggg cccaacctca
    21121 atctcctcgg cacacgcgag ccgcacttgt acgggcgcga tacgctggcg cagatcgagc
    21181 ggcggcttgc tgcgcgcgcc aaggccgccg gcgtcgggct ggcgtgcctg cagaccaacc
    21241 acgaaggcat cgccgtcgac cgcgtacagc aggcacagcg cgagcgcgtc ggcttcatca
    21301 tcctcaaccc ggccgctttc acctactcca gcatcgcgct gcgcgacgcg ctgacggcgg
    21361 tgcagattcc gttcatcgag gtgcacctgt cgaacatcca cgcgcgcgag ccctggcgcg
    21421 cgcattcgca tttcacggcc gtggcggtcg gctcgatcct aggcctgggc agccgcggct
    21481 acgacctggc gctcgagtac gcgatcgagc gcctcaaggg cggcgccggg gcctagcccc
    21541 ggcgtgctcg cggcgggccc gacggccctc cggaggagac cacagcatgg atctgcgcaa
    21601 gctgaagaag ctcatcgacc tggtgcagga atcgggtatc gccgagctcg agatcaccga
    21661 gggcgaagag aaggtgaaga tcgtgaaagg cggggaggcg acggtgacgc cgctcgcgcc
    21721 ggccgccccg gcgccaggcg ccgccgcccc ggc
//
LOCUS       DBNHKE010000069        11671 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_329023, whole genome shotgun sequence.
ACCESSION   DBNHKE010000069 DBNHKE010000000
VERSION     DBNHKE010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_329023"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16O9_04500"
     CDS             complement(<1..217)
                     /locus_tag="AC16O9_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6515911.1"
                     /translation="MIRVLLADDHQIVRDGLRRILAAQADVEVAGEAADGDQALALVR
                     AHDYDVAVLDLSMPGLAGLDLVKRLKLE"
     gene            complement(214..1455)
                     /locus_tag="AC16O9_04505"
     CDS             complement(214..1455)
                     /locus_tag="AC16O9_04505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6515912.1"
                     /translation="MAGGAALAGLIAGNVAAFMVGMASPWLLGGVILADMLAVSLCVT
                     GALWSIAVWGERREVSEARLDAIVDSAMDAIITVDAAQNIVLFNRAAEQMFGVARDEA
                     HGTPLERFIPARFRGAHRGHFEKFGQTGVTSRRMGDATTLRALRANGEEFPIEASISQ
                     AGEPGARFFTVILRDTSLRRKHEETLLRQQRELRELSARVLEAREEEKTRIARELHDE
                     LGQLLTALKMDLAWLRERLPAGGTELATRADGMGALLDRTVGASRRIAADLRPLMLDD
                     LGLAEAAQWLVEDFGKRSGVRLEMQMAQDAAFEALSKGAATALYRALQESLTNIARHS
                     GAKHAWVVLAQENGALHLEIEDDGRGVSAEDLEKSSSLGLKGMRERVAYYGGTLEVAR
                     APRGGTRLRVRMPLASGTAES"
     gene            1536..2930
                     /gene="hemN"
                     /locus_tag="AC16O9_04510"
     CDS             1536..2930
                     /gene="hemN"
                     /locus_tag="AC16O9_04510"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIR6515913.1"
                     /translation="MHYPAADLVIDPVLIRQYDVSGPRYTSYPTADRFVEAFGETELR
                     QWLAKRSIGGVGQPLSLYVHLPFCDTLCYYCGCNKVVTRDHTKSSKYIKYLEKELALL
                     DPALGNVREISQIHWGGGTPTFLSHEEMRTLVAAIDRHFRRAPDCESAIEVDPRSAAP
                     GTLELLAALGFNRVSLGVQDFDPQVQKAVHRLQSEEETRRVVGEARANGFRSVNIDLI
                     YGLPRQTLDGFNRTLDRVIALDPDRIALYSYAHLPKVFMPQRRIAEADLPSAEAKLQI
                     LSLAIGRLTRAGYLYIGMDHFARPDDELALAQSQGRLQRNFQGYSTRRGSDLIGLGVS
                     AIGAIGPTYYQNQKRLDDYYAALDAGRLPVARGVELTPDDLLRRAVIHGLACHFRLSV
                     ESVELSYLVDFKRYFAPEMARLQRLEAEGLVELQPDWIVVTPKGRLLVRVVCAVFDRY
                     LREREARAQYSKVI"
     gene            2932..3654
                     /locus_tag="AC16O9_04515"
     CDS             2932..3654
                     /locus_tag="AC16O9_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6515914.1"
                     /translation="MDVLLPAMLAFGLASGLHCVGMCGGIVAAFSARRVVPIAAARRP
                     AREWRRQIAFNGGRILAYALGGAVAGTLGGAAALVQGALPAQLALFVLAQGVLILAGL
                     YLLGAGRLLARVERFGVPLWRRVQPVAARLATAPGTPAAGLAGMLWGLLPCAMVYTAL
                     ATSTLAGGPVRGALAMLAFGVGTLPWLLGAGALLARLGAGAGMARLRAAAGVLVLGMG
                     VASLARAEDLRAAVRAGLLCLT"
     gene            3668..4090
                     /locus_tag="AC16O9_04520"
     CDS             3668..4090
                     /locus_tag="AC16O9_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6515915.1"
                     /translation="MKRILVPVDGSEASLRALRHAAQRAREAQGEVHLLHVEPPMAYE
                     ELRAYALRPELEQLREDACGRVLKAAAATLAAEKVPCAEHLQRGEVAHTIARFASAHQ
                     IDEVVMGTRGMGALGAIFVGSVAQRVVHLADVPVTLVK"
     gene            4090..4686
                     /locus_tag="AC16O9_04525"
     CDS             4090..4686
                     /locus_tag="AC16O9_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR6515916.1"
                     /translation="MTAMRDAVRLLRDEHRTISAVLSGLRELARLAQEPGVRPDFAAL
                     RAMVYYIDTFPEKLHHPKEDEHLFARLVQRAPEAAPLVDELQAEHVAGARLVRDLERA
                     VLGYEQSWPEGGVAFRDAVEAYAQFHWRHMRKEELSLLPLAEERLAAEDWEAIAAAFR
                     ANQDPLAGTAERDFSRLFQRIAEIAPAPVGLGTPWKAR"
     gene            complement(4683..5129)
                     /locus_tag="AC16O9_04530"
     CDS             complement(4683..5129)
                     /locus_tag="AC16O9_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6515917.1"
                     /translation="MFKHILVTTDGSQLSLKGIKAGVRLAAALGAKVTGVCVIPPWRT
                     PVFSEGVTYGVPRSPAQYREATEKAARKALAAVAIEAETAGVPCTTQFVTSERPWEGI
                     LRVAKAKRCDLVSIASHGRGGLGGLLLGSETQRLLAHSKLPVLVTR"
     gene            complement(5194..5877)
                     /locus_tag="AC16O9_04535"
     CDS             complement(5194..5877)
                     /locus_tag="AC16O9_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6515918.1"
                     /translation="MSTPPRERLALGVLARLPLFEGVSRPHFSEVIRHARTLHYQRAQ
                     VVAKRGEMVPGLFAVAYGMVKLTLHGATGAEKVLQLVGPSQTFGEIALFRQQPLPLDA
                     VAVVDTGLVLVPAATLLALIDRDAHFARGMVASLCQRIHAMVSDFEATSLHGASGRLA
                     AYLESLAETPDTVRVTLPAAKKLIAARLGMTKETFSRALRELAREGLVRVTGRSIELL
                     DRARLARRD"
     gene            complement(5874..6062)
                     /locus_tag="AC16O9_04540"
     CDS             complement(5874..6062)
                     /locus_tag="AC16O9_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515919.1"
                     /translation="MDIAWFTLVAVALYFGADRLLDLIERLRGARFENRQVAFFAIIL
                     PLALVAFWLVRLLTGPAP"
     gene            complement(6035..7429)
                     /locus_tag="AC16O9_04545"
     CDS             complement(6035..7429)
                     /locus_tag="AC16O9_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR6515920.1"
                     /translation="MSSLFADGIAPAARYALAAPGDARRRLATGWLWLAVAALVGAGL
                     FSVLLVLARAPYTKELFATADFFRVALVVHVDLSVLVWFAAFGGLLWTLNGGTRHLGL
                     GWAGLAAAAAGTLAMCAAPFLGTEPPVMSNYVPVLDGPVFLGGLLLFAAGCVLLVVRA
                     MLAAERVGTRPDGAAALRFGLNAAAVSAAVALLAFAWSYAALPARLEGKAYYEVLFWG
                     GGHVLQFTWTLLMFAAWLALAEAGGARAPLSPRVALLLFAIALAAVFATPVIYLAYDI
                     TSVEHYRLQTWLMRIGGGLAIVPFALATLTALATAPRAEPAQRPLRAALWGSLALFGA
                     GGLIGLAIEGSNVKIPAHYHGAIVGVTLAFMGLAYHLLPRLGGARPEPRLATVQAWLY
                     GAGQLAHIAGLVWSGGYGVQRKVAGAEQVLRGTEQIAAMGLMGAGGLAAVAGGVLFIF
                     IVLRSLWTSPGSRS"
     gene            complement(7410..8795)
                     /locus_tag="AC16O9_04550"
     CDS             complement(7410..8795)
                     /locus_tag="AC16O9_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR6515921.1"
                     /translation="MASSFAPENRDRPHFSSRWKNEVCPHFLAPWRHLGAIGFALFWM
                     LAASGIYLYIRFDTSVAGAYDSIDELEWWFGGVLRSLHRYAADAFVVVVVLHVVRELA
                     AGRFRGFRAFTWISGVPLVWLLYASGIVGYWLVWDARAQFSAQATAEWLLALGIGAEP
                     MARNFLGAESITDRLFSLFVFLHIGLPLALLAGMWVHVQRLGRPATVAPRALAWGLAG
                     ALVALALLAPVSSGARWDPAAVPQALTMDWFLLFLHPLMYATSPALLWLAVGGATAAL
                     AALPYAAPRLRAPVARVDPVNCNGCGRCVADCPYEAVTMVGKRARVYDERCAACGICA
                     GACPSATPFRSVTELASGIDLPDLTVDELRRRMQRALPADELVFSCEGARAPGIHLRC
                     LAMLPPSFIEYALRHGARRVRAIGCPDGECAWRTGLDLAAERFAGAREPYLRPTVACS
                     KQGPAYEFALR"
     gene            complement(8773..9276)
                     /locus_tag="AC16O9_04555"
     CDS             complement(8773..9276)
                     /locus_tag="AC16O9_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515922.1"
                     /translation="MKQALRIAAQLALYVPLMALIGYFSTEPRFFTLAPDQALVRLSF
                     IHAAERKEPCRQRSAEELAKLAPNMRAALDCPRERAVLLVELEVDGEIVLRREVAPSG
                     VQRDGNATVYHRLPLAAGRHRIVARLRDGPGEEFNFIGETTLELAPGAALLIDFSRAK
                     GGFVFRT"
     gene            complement(9273..11183)
                     /locus_tag="AC16O9_04560"
     CDS             complement(9273..11183)
                     /locus_tag="AC16O9_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6515923.1"
                     /translation="MIHRIHLIGQWFFLRVEALFNLAFGDRLNPLYYLGPIAYYLMWL
                     VVASGLYLYVLFETSVGGAYGSVQALTLDQPYAGGVLRSVHRYASDGVVLVMALHLAR
                     HWCFDRYRGFRWFSWVSGVALLWLVYTSGINGYMLPWDRLSQFVVVATAEWLDWLPVF
                     QGALVRNFIFAENLNDRLFSLLSFIHIGVPLAVLALLWIHTQRVPQARTAPPLPVTLT
                     LTAALVVLSLVKPAVSQPRADLATAAGSVAFDWFYLPVYALLYRTSPGEVWLLLGGAT
                     VLFLLLPWLPPKKKAAEHHMLIRPDNRIVSVRAGDTILDAALRDGIALPFECRHGGCG
                     QCKATLAYGEVDYGAYQQEALPRAERDAGKILTCCATPRGDVEIEYLPAKTPGGIRPR
                     EWRATVESLERLAPDVMRVILAIEGGEPISFYAGQYINVLLEDGARRSFSFATAPQQS
                     ARIELHIRHIPGGRYTGHVFEGMRTGDRLRFEGPLGAFFLREDTDKPIIFVAGSTGFA
                     PVKSMLEYAFAKGLRRRMLLYWGTRQRADMYLAKLPEQWAREHDNFTFVPVLSDPAPE
                     DRWQGRTGLVHEAILADFPSLAGHQVYACGSSAMVEAAHPAFRAHGLAQDDCFADAFR
                     PAPQLARLGGHA"
     gene            complement(11228..>11671)
                     /locus_tag="AC16O9_04565"
     CDS             complement(11228..>11671)
                     /locus_tag="AC16O9_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6515924.1"
                     /translation="KEFRRKHAVESRNWLFLSPEAQTLPQLVADFGFRYESTASGFDH
                     LLQASIVDANGRIYRQVYGDQFEVPLFVGPLLQLAQAKPVPQGNLQAMLEQVKLLCTV
                     YDPVAGRYRVNYVIVAELLVGASIILGILAFLVVEWRRRRRPSNI"
BASE COUNT         1821 a         4055 c         4234 g         1561 t
ORIGIN      
        1 tctcgagctt caagcgcttg acgaggtcga gcccggcgag gcccggcatc gacaggtcga
       61 gcacggcgac gtcgtagtcg tgggcgcgca ccagcgcgag cgcctggtcg ccgtctgcgg
      121 cctcgcccgc gacctcgacg tcggcttgcg cggcgaggat gcggcgcagg ccgtcgcgga
      181 cgatctggtg gtcgtcggcg agcaggaccc ggatcacgac tcggcggtcc cggaagcgag
      241 cggcatgcgc acgcgcagcc gcgtgccgcc gcgcggtgcg cgcgccacct cgagcgtgcc
      301 gccgtagtag gcgacgcgct cgcgcatgcc cttcaggccg agcgagctcg acttctcgag
      361 gtcctcggca ctgacgcctc ggccatcatc ctcgatctcc aggtgcaggg cgccgttctc
      421 ctgcgccagg acgacccagg cgtgcttcgc gccggagtgg cgcgcgatgt tggtgagcga
      481 ctcctgcagc gcccggtaaa gcgcggtggc ggcgcccttg gacagcgcct cgaaggcggc
      541 gtcctgcgcc atctgcatct cgagccgcac gccggagcgc ttgccgaagt cctcgaccag
      601 ccactgcgcc gcctcggcca gccccaggtc atcgagcatc agcggccgca gatcggcggc
      661 aatgcgccgg ctcgcgccca cggtgcggtc gagcagggcg cccatgccgt cggcgcgcgt
      721 cgcaagctcg gtgccgccgg ccggcaggcg ctcgcgcagc caggcgaggt ccatcttcaa
      781 cgcggtgagc agctgcccga gctcatcgtg tagctcgcgc gctatgcgcg tcttctcctc
      841 ttcgcgcgcc tccagcacgc gcgccgaaag ctcgcgcagt tcgcgctgct ggcgcagcag
      901 cgtctcctcg tgcttgcggc gcaggctggt gtcgcgcagg atcaccgtga agaagcgtgc
      961 gccgggctcg cccgcctgcg agatcgaggc ctcgatgggg aactcctcgc cgttcgcacg
     1021 cagcgcccgc agcgtcgtgg cgtcgcccat gcgccggctg gtgacgccgg tctggccgaa
     1081 cttctcgaaa tgcccgcggt gcgcgccgcg aaagcgcgcc gggatgaagc gctcgagcgg
     1141 cgtgccgtgc gcctcgtcgc gcgcgacgcc gaacatttgc tcggcggcgc ggttgaacag
     1201 cacgatgttc tgcgccgcgt ctaccgtaat gatcgcgtcc atcgccgaat ccacgatggc
     1261 gtcgaggcgc gcctcgctca cctcgcggcg ctcgccccag accgcaatcg accacagcgc
     1321 tcccgtcacg cagagcgaga ccgccagcat gtcggcgagg atcaccccgc ccaggagcca
     1381 gggcgacgcc atgccgacca tgaaagcggc gacattgccg gcaatgaggc cggcgagcgc
     1441 cgccccgccg gccaccaagg tcgccgggtg gcggaaaagg cttcgtttga cggaaatcaa
     1501 agtgcgcgac cctttcctct tcaaagtgat gaccaatgca ctatcccgcc gccgatctgg
     1561 tcatcgaccc ggtcctcatc cgccagtacg acgtgagcgg gccgcgctat acctcgtatc
     1621 cgaccgccga ccgcttcgtc gaggcttttg gcgagaccga actgcgccag tggctcgcca
     1681 agcgcagcat cggcggcgtc ggccagcccc tgtcgctcta cgtgcacctg ccattctgcg
     1741 acacgctctg ctactactgc ggctgcaaca aggtggtgac ccgcgaccac acgaaatcgt
     1801 ccaagtatat caagtacttg gagaaagagc tggcgctgct ggacccggcg ctgggcaacg
     1861 tgcgggaaat cagccagata cactggggcg gcggtacgcc gacgttcctt tcgcacgagg
     1921 aaatgcgcac cctggtggcg gcgatcgacc ggcatttccg gcgtgcgccc gactgcgagt
     1981 cggcgatcga ggtggacccg cggagtgcgg cgcccggcac cctggagctt ctcgccgcgc
     2041 tcggcttcaa ccgcgtctcg ctcggcgtgc aggacttcga cccgcaggtg cagaaggcgg
     2101 tgcaccgcct gcagagcgag gaggagacgc gccgcgtggt gggcgaggcg cgcgccaatg
     2161 gctttcgctc ggtgaacatc gacctgatct atgggctgcc caggcagacg ctggacggct
     2221 tcaaccgcac gctcgaccgg gtgatcgccc tggatccgga ccgcatcgcg ctgtacagct
     2281 acgcgcacct gccgaaggtg ttcatgccgc agcggcgcat cgccgaagcc gacctgcctt
     2341 cggcggaggc gaagctgcag atcctgtcgc tcgccatcgg gcgcctgacg cgcgcggggt
     2401 acctctacat cggcatggac cacttcgcgc gcccggacga cgaattggcg ctcgcgcagt
     2461 cgcagggccg tctgcagcgc aacttccagg gctactcgac gcgccgcggc agcgacctga
     2521 tcggcctggg cgtctcggcg atcggcgcga tcggcccgac ctactaccag aaccagaagc
     2581 gcctggacga ctactatgcc gcgctcgacg ccggccgcct gccggtggcc cgcggcgtcg
     2641 agctgacgcc ggacgacctg ctgcgccgcg cggtgatcca cgggctggcg tgccacttcc
     2701 gcctgtccgt cgaatccgtg gagctgtcct acctcgtgga cttcaagcgc tacttcgcgc
     2761 cggagatggc gcgcctgcag cggctcgaag ccgagggcct ggtcgagctg cagccggatt
     2821 ggatcgtggt caccccgaag gggcggctcc tggtgcgcgt ggtgtgcgcc gtgttcgacc
     2881 gctacctacg cgaacgcgaa gcgcgggcgc agtactccaa ggtcatctaa agtggacgtg
     2941 ctgctgccgg cgatgctcgc gttcggcttg gcatcgggcc tgcactgcgt cggcatgtgt
     3001 gggggcatcg tcgccgcttt ctcggcgcgg cgggtggtgc cgatcgcggc ggcgcggcgc
     3061 ccggcccgcg aatggcgccg gcagatcgca ttcaacggcg gccgcatcct ggcctacgcg
     3121 ctggggggcg ccgtggcggg tacgctgggc ggcgcggcgg cgctcgtgca gggcgccttg
     3181 cccgcgcaac tggcgttgtt cgtgctcgcg cagggcgtgc tgatcctcgc cggcctgtac
     3241 ctcctgggcg cgggccggct gctcgcgcgc gtcgagcgct tcggcgtgcc gttgtggcgg
     3301 cgcgtgcagc cggtggcggc gcgcctggcg accgcgcccg gcacgccggc ggccgggctc
     3361 gccggcatgc tctgggggct gctgccttgc gcgatggtgt acacggcgct cgccacgtcg
     3421 acgctcgcgg gcgggcccgt gcggggcgcg ctcgccatgc tcgcctttgg cgtcggcacg
     3481 ctgccgtggc tgctcggcgc gggcgcgctg ctcgcgcggc tgggcgcggg cgccggcatg
     3541 gcgcggctgc gcgcggccgc cggcgtgctc gtgctcggca tgggggtggc gagcctggcg
     3601 cgcgcggagg acctgcgcgc cgcggtgcgt gcggggctac tgtgccttac ctgagataat
     3661 gcaggagatg aaacgcatcc tcgtgccggt ggatggctcc gaggcctcgc tgcgggcgtt
     3721 gcgccacgcc gcgcagcgcg cgcgcgaggc gcagggcgag gtgcacctgc tgcacgtcga
     3781 gccgccgatg gcctacgagg agctgcgcgc ctacgcgttg cgcccggaac tggagcaact
     3841 gcgcgaggac gcctgcgggc gcgtcctgaa agccgcggcc gccacgctgg cggcggagaa
     3901 ggtgccctgc gccgagcacc tgcagcgcgg cgaagtggcg cacaccatcg cgcgcttcgc
     3961 ctcggcgcac caaatcgacg aggtggttat gggaacacgc ggcatgggcg cgcttggcgc
     4021 gatattcgtc ggctcggtgg cgcagcgcgt ggtgcacctc gccgacgtgc cggtgacgct
     4081 ggtgaaatga tgacggcgat gcgcgacgcg gtgcgtcttc tgcgcgacga gcatcgcacg
     4141 atcagcgccg tgctgagcgg cctgcgcgag ctggcgcgcc tggcgcagga gccgggcgtg
     4201 cgccccgact tcgcggcgct gcgcgccatg gtctattaca tcgacacgtt tcccgagaag
     4261 ctgcaccacc cgaaggagga cgagcacctg ttcgcgcggc tggtgcagcg cgcgcccgag
     4321 gccgcgccgc tggtcgatga gctgcaggcc gagcacgtcg cgggggcgcg cctggtgcgc
     4381 gatctcgagc gcgcggtgct cggctacgag cagtcctggc ccgaaggcgg cgtcgcgttc
     4441 cgcgacgcgg tcgaggccta cgcccagttc cactggcgtc acatgcgcaa ggaggagctg
     4501 tcgctgctgc cgcttgccga ggagcggctc gcggccgagg actgggaggc gatcgcggcg
     4561 gcgttccgcg ccaaccagga tccgctcgcg gggaccgcag agcgcgactt cagccgcctg
     4621 ttccagcgga tcgcggagat cgcgccggcg cccgtcggcc taggcacgcc ctggaaagcg
     4681 cgctagcgcg tcaccagcac gggcagcttg gagtgcgcga gcaggcgctg ggtctcgctg
     4741 ccgagcagta gaccgcccag cccgccgcgg ccgtgcgagg cgatggacac caggtcgcag
     4801 cgcttggcct tcgccacgcg caggatgcct tcccagggcc gctcggacgt gacgaactgc
     4861 gttgtgcaag gcacgccggc cgtctcggcc tcgatcgcca cggcggcgag cgccttgcgc
     4921 gcggcctttt ccgtcgcctc cctgtactgc gcgggcgagc ggggcactcc gtaggtgacg
     4981 ccctcgctga acacgggcgt gcgccacggc gggatcacgc agacgcccgt gaccttggcg
     5041 ccgagcgcag cagcgaggcg caccccggcc ttgatcccct tcagggacag ctgcgagccg
     5101 tcggtagtga ccaggatgtg cttgaacatg aagcctccgc gactatggat ggccccagta
     5161 tgacccaggg tgccgcgccg gggttgactt gcgtcaatcg cgtcgggcca gccgggcgcg
     5221 gtcgagcagc tcgatgctgc gccccgtcac gcgcaccagc ccttcgcgcg ccaactcgcg
     5281 cagcgcgcgc gagaacgtct ccttcgtcat gccgagccgc gccgcgatca gcttcttcgc
     5341 cgccggcagc gtcacgcgca cggtgtccgg cgtttccgcg agcgactcga ggtacgccgc
     5401 gaggcgcccg ctggcgccgt gcaggctggt ggcctcgaag tcgctcacca tggcgtggat
     5461 gcgctggcac aggctcgcca ccatgccgcg cgcgaagtgc gcgtcccggt cgatcagcgc
     5521 gagcagcgtc gccgccggca ccaggacgag gcccgtgtcc accaccgcca ccgcgtcgag
     5581 tggcagcggc tgctgccgga acagcgcgat ctcgccgaag gtctgcgacg gtcccaccag
     5641 ctgcagcacc ttttcggcgc cggtggcgcc gtgcagcgtg agcttgacca tgccgtaggc
     5701 cacggcgaaa agccccggca ccatctcgcc gcgctttgcc accacctgag cgcgctggta
     5761 atgcagcgtg cgcgcgtggc gtatcacttc gctgaaatgc gggcgcgaga cgccttcgaa
     5821 cagcggcagc cgggcgagca cgcccagcgc caggcgctcg cgtggcgggg tgctcatggc
     5881 gccggcccgg tcagcaagcg caccagccag aatgcgacca gcgcgagcgg caggatgatg
     5941 gcgaagaacg ccacctgccg gttctcgaag cgcgcgccgc gcagccgctc gatcaggtcg
     6001 agcaggcggt cggcgccgaa gtacagcgca acggctacga gcgtgaacca ggcgatgtcc
     6061 atagggaacg cagcacgatg aagatgaaga gtaccccgcc ggcgacggcg gcgaggccgc
     6121 cggcacccat caggcccatg gccgcgattt gctcggtgcc gcgcagcacc tgctcggcgc
     6181 cggccacctt gcgctgcacg ccatagcctc ccgaccagac caggccggcg atgtgcgcga
     6241 gctgccccgc gccgtagagc caggcctgca cggtggcgag gcgcggctcc gggcgcgcgc
     6301 cgcccaggcg cggcagcagg tggtaagcga gtcccatgaa ggcgagggtc acaccgacga
     6361 tggcgccgtg gtagtgcgcg gggatcttga cgttgctgcc ctcgatcgcc agcccgatca
     6421 ggccaccggc gccgaagagc gcgagcgagc cccacagcgc ggcgcgcaag gggcgctggg
     6481 cgggctcggc gcgcggcgcg gtggcgagcg cggttagcgt cgccagggcg aagggcacga
     6541 tggcgaggcc cccgccgatg cgcatgagcc acgtttgcag ccggtagtgc tcgaccgagg
     6601 tgatgtcgta ggcgaggtag atcaccggcg tggcgaacac cgcggcgagc gcgatggcga
     6661 acagcagcag cgcgacccgc ggcgagagtg gcgcgcgcgc gccgcccgcc tcggcgagcg
     6721 ccagccacgc ggcgaacatg agcagcgtcc aggtgaattg cagcacgtgg ccgccgcccc
     6781 agaacagcac ttcgtaatac gccttgccct cgagccgagc gggcagcgcg gcgtacgacc
     6841 aggcgaaggc gaggagcgcg accgccgccg agactgcggc cgcgttcagc ccgaagcgca
     6901 gtgccgcggc gccgtccggg cgtgtgccga cgcgctcggc agcgagcatc gcgcgcacaa
     6961 cgagcagcac gcagcctgcg gcgaagagca gcaggccgcc gagaaacacg ggcccgtcga
     7021 gcaccggcac gtagttgctc atcaccggcg gctcggtgcc aaggaagggc gcagcgcaca
     7081 tggcgagcgt ccccgccgcc gctgccgcga gcccggccca gcccaggccg aggtggcgcg
     7141 tgccgccgtt cagcgtccac agcagcccgc cgaaggcggc gaaccacacc agcaccgaca
     7201 ggtcgacgtg caccaccagc gcgacgcgga agaagtcggc cgttgcgaac agctccttgg
     7261 tatagggcgc gcgcgcgagc accagcagca cggagaacag gccggcgccc acgagcgcgg
     7321 ccaccgccag ccacagccag ccggtcgcga ggcgccgccg cgcgtcgccg ggtgcggcga
     7381 gcgcatagcg cgccgcgggt gcgatgccgt cagcgaagag cgaactcata ggcagggcct
     7441 tgtttcgaac aggcgacggt ggggcgcagg tacggctcgc gtgcgccggc aaagcgctcc
     7501 gccgccaggt cgaggccggt gcgccaggcg cactcgccgt cggggcagcc gatcgcccgc
     7561 acgcgccgcg cgccgtggcg cagcgcgtac tcgatgaagg agggcggcag catcgccagg
     7621 cagcgcagat ggatcccggg ggcgcgggcg ccttcgcagc tgaacacgag ctcgtcggcc
     7681 ggcagcgcgc gctgcatgcg gcggcgcagc tcgtccaccg tcaggtccgg gagatcgatg
     7741 ccggaggcga gctcggtcac gctgcggaac ggcgtcgccg aggggcaggc gccggcgcag
     7801 atgccgcagg ccgcgcaacg ctcgtcgtac acgcgcgcgc gcttgccgac catggtcacg
     7861 gcctcgtacg ggcaatcggc gacgcagcgc ccgcagccgt tgcagttgac agggtcgacc
     7921 cgggcaacgg gtgcgcgcag gcgcggcgcg gcgtaaggca gcgccgcgag cgccgcggtg
     7981 gcgccgccca cggcgagcca caggagcgcg ggcgacgtcg catacatgag cggatgcaga
     8041 aacagcagga accagtccat cgtcagcgct tgcggcaccg cggcggggtc ccagcgcgcc
     8101 ccggaggata cgggcgcgag cagcgccagc gcaaccagcg cgccggcgag gccccaggcc
     8161 agtgcgcgcg gggccacggt cgccggccgc ccgaggcgct gcacgtgcac ccacattccg
     8221 gcgagcagcg cgagcggcag gccgatgtgc aggaacacga acagcgagaa cagccggtcg
     8281 gtgatgctct cggcgccaag gaagttgcgc gccatcggct cggcgccgat gcccagggcg
     8341 agcagccatt ctgcggtggc ctgcgcggag aactgcgcgc gcgcatccca caccagccag
     8401 tagccgacga tgccggaggc gtagagcagc cacaccagcg gcacgccgga gatccaggtg
     8461 aaggcgcgaa agccgcgaaa gcgcccggcg gcgagctcgc gcacgacatg cagcaccact
     8521 accacgacga aagcatcggc ggcgtagcgg tgcaggctgc gcagcacgcc gccgaaccac
     8581 cattcgagct cgtcgatcga gtcgtaggcg ccggcgacgc tggtgtcgaa gcggatgtac
     8641 agatagatgc cgctcgcggc cagcatccag aacagcgcga agccgatcgc gccaaggtgc
     8701 cgccacggcg ccagaaaatg gggacagacc tcatttttcc aacgggaaga aaaatgaggt
     8761 ctgtccctat tttcaggtgc gaaagacgaa gccacctttg gccctggaga agtcgatgag
     8821 gagcgcggcg ccgggcgcga gctcgagcgt tgtttcgccg atgaagttga attcctcgcc
     8881 ggggccgtcg cgcaggcgcg cgacgatgcg gtgccggccc gcggcgagcg gcaggcggtg
     8941 gtacacggtg gcgttgccgt cgcgctgcac gcccgagggc gccacctcgc gccgcagcac
     9001 gatttcgccg tcgacctcca gctcgacgag cagcaccgcg cgctcgcgcg ggcagtcgag
     9061 cgccgcgcgc atgttgggcg cgagcttggc gagctcttcg gcgctgcgct ggcggcatgg
     9121 ctctttgcgc tcggcggcgt ggatgaacga caggcgcacc agggcctggt ccggcgcgag
     9181 cgtgaagaag cgcggctcgg tggagaagta gccgatcagc gccatgagcg gcacataaag
     9241 cgcaagctgc gcggcgatgc gcagggcttg cttcatgcgt ggcctccgag gcgcgcgagc
     9301 tgcggcgcgg ggcggaaagc gtcggcgaag cagtcatcct gcgccagccc gtgcgcgcgg
     9361 aatgccggat gggcggcttc gaccatcgca ctcgagccgc aggcgtacac ctggtggccg
     9421 gccaggctgg ggaagtccgc caggatcgcc tcgtgcacga ggccggtgcg gccctgccag
     9481 cggtcctcgg gcgcggggtc ggagagcacc ggcacgaagg tgaagttgtc atgctcgcgc
     9541 gcccattgct cgggcagctt ggcgaggtac atgtccgcgc gctgccgcgt gccccaatac
     9601 agcagcatgc ggcgccgcag ccctttggcg aaggcgtact ccagcatgct cttcaccggc
     9661 gcgaagccgg tcgagccggc gacgaagatg atcggcttgt cggtgtcctc gcgcaggaag
     9721 aaggcgccga gcggtccctc gaagcgcagc ctgtccccgg tgcgcatccc ctcgaacacg
     9781 tggccggtgt agcgcccgcc cgggatgtgc cggatgtgca gctcgatgcg cgcgctctgc
     9841 tgcggcgcag tggcgaagga aaagctgcgc cgcgccccgt cctcgagcag cacgttgatg
     9901 tactgcccgg cgtagaacga gatgggctca ccgccttcga tggcgaggat cacgcgcatc
     9961 acgtcggggg cgagccgctc gaggctttcc accgtggccc gccattcgcg cgggcggatg
    10021 ccgcccgggg tcttcgccgg caggtactcg atctcgacgt cgccgcgcgg cgtggcgcag
    10081 caggtgagga tcttgccggc gtcgcgctcg gcccgcggca gcgcctcctg ctggtaggcg
    10141 ccgtaatcga cttcgccata ggcgagcgtc gccttgcact gcccgcagcc gccatggcgg
    10201 cactcgaacg gcagcgcgat gccgtcgcgc agcgcggcat caaggatcgt gtcgcctgca
    10261 cgcaccgaga cgatgcgatt gtcggggcgt atcagcatat ggtgctcggc cgctttcttc
    10321 ttcggcggca gccagggcag caggagaaac agcaccgtgg cgccgccgag cagcagccag
    10381 acttcgcccg gcgaggtgcg gtagagcagc gcgtacaccg gcaggtagaa ccagtcgaac
    10441 gcgacgctgc cggccgcggt ggcgagatcg gcgcgcggct ggctgacggc gggcttgacg
    10501 agcgagagca cgaccagtgc cgcggtcagc gtgagcgtca ccggcagcgg cggcgcggtg
    10561 cgcgcctgcg gcacgcgctg cgtatggatc cagagcagcg ccagcaccgc gagcggcacg
    10621 ccgatgtgga tgaaggagag cagcgagaac aggcggtcgt tcaggttctc ggcaaagatg
    10681 aagttgcgca ccagcgcgcc ctggaacacc ggcagccagt cgagccattc ggcggtggcg
    10741 acgaccacga actgcgacag gcgatcccag ggcagcatgt agccgttgat ccccgaggtg
    10801 taaaccagcc acagcagcgc gacgccgctt acccacgaga accagcgaaa gccgcggtag
    10861 cggtcgaagc accagtggcg cgcgaggtgc agcgccatca ccagcaccac gccgtcggag
    10921 gcataacggt ggaccgagcg cagcacgcct cctgcatacg gctggtcgag cgtcaacgcc
    10981 tgcaccgagc cgtaggcgcc gccgacgctg gtctcgaaga gcacgtacag gtacagcccg
    11041 ctcgccacca ccagccacat caggtagtag gcgatcgggc caaggtagta cagcgggttc
    11101 agccggtcgc cgaaggcgag gttgaacagc gcctccaccc tgaggaaaaa ccactgtccg
    11161 atcaggtgta tacggtgaat caccgccaca tcctagggcg gtggtggggc gcgcaggttg
    11221 atctgggtta aatattcgac ggtcgccgcc gccgccgcca ctcgacgacc aggaacgcca
    11281 ggatgccgag gatgatgctc gcgccgacga gcagctcggc gacgatcaca tagttgaccc
    11341 ggtagcgccc ggccacgggg tcgtacacgg tgcacagcag cttcacctgc tcgagcatcg
    11401 cctgcaagtt gccctgcggc acgggcttcg cctgcgcgag ctgcagcagc ggcccgacga
    11461 acagcggcac ctcgaactga tcgccgtata cctgccggta gatgcggccg ttggcgtcga
    11521 cgatgctcgc ctgcagcagg tggtcgaacc cggaggcggt cgactcgtag cgaaagccga
    11581 aatcggcgac gagttgcggc agcgtttgcg cctcgggcga gaggaacagc cagttgcgcg
    11641 actccactgc gtgcttgcgc ctgaactcct t
//
LOCUS       DBNHKE010000070        18328 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1387470, whole genome shotgun sequence.
ACCESSION   DBNHKE010000070 DBNHKE010000000
VERSION     DBNHKE010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1387470"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1385)
                     /locus_tag="AC16O9_04570"
                     /pseudo
     CDS             complement(123..1385)
                     /locus_tag="AC16O9_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(1411..2661)
                     /locus_tag="AC16O9_04575"
     CDS             complement(1411..2661)
                     /locus_tag="AC16O9_04575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6515925.1"
                     /translation="MRSVVESVLRKAGERSSAPFAVRFAGGGEWRSRDGAPAFTVVFH
                     RAAAYARIAAFGHIGLLESYFDGDLDIEGSLAAAMAVGLVGGVAGANPLVGLRNRWHE
                     FRFSNRSWQQAKKNAEFHYALGTEFYRYWLDDPHMMYTCAYWKEGTKTLEQAQQNKID
                     HVARKVRLAPGETVVDIGSGFGGFMFRAHDKFGVKVTGINNTTSQVEMVRAQIARRGL
                     GGALDVIDTDFRDTRRQYDKVVSIGTLEHAGRDALEEVVRAHAAFLKPGGLGMLHFIG
                     HVGHFQTEFFIRKHVFPGGWIPSLAHTIAAMERAGLEVVDIENLRRHYALTLDAWGER
                     FDRHWPQIQALDPKKFDERFRRVWRVYLYGCAEMFRSAAGYTHLFQIVFSKGNITRKN
                     YPMSREFLYEQRAHREDAGAQRAA"
     gene            complement(2691..3521)
                     /locus_tag="AC16O9_04580"
     CDS             complement(2691..3521)
                     /locus_tag="AC16O9_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6515926.1"
                     /translation="MTYKTILVHFDHHPRSTERLGLACSLADEFDAHIVGLFASGLQR
                     LPSYALAEGGPLLRDMLEKRNAEVAAEAERRFRDVARRNGGRTEWRSTQSDAASAMRL
                     SARYADLVVAGQPEATYEGDLRGLADELVFSAGRPVLFVPYAGRFPMLGKRVLVAWDG
                     GREAARAVTDALPLLRRAEAVEVCAFDPEKRLNSHGQQPGADVGLYLARHGVKVTVAR
                     QSGAGYGVGAQILSRAADTGADLIVMGAYGHARVREMVLGGATRTLLEAMTVPVLMSH
                     "
     gene            complement(3587..5209)
                     /gene="groL"
                     /locus_tag="AC16O9_04585"
     CDS             complement(3587..5209)
                     /gene="groL"
                     /locus_tag="AC16O9_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689592.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6515927.1"
                     /translation="MPAKQLLFGETAHARLVKGMNTLADAVRVTLGPKARTVVLEKSW
                     GAPIVINSGVIVAREIELADPFENMGAQMVREVASKTSEVAGDGTTTATLLAAAVVTE
                     GMKYVAAGMNPMDLKRGIDLAVEGVVGELKRMAKPCATRKEIAQVGAISANNDASIGE
                     MIAEAMEKVGREGVITVEDGKGLANELEVVEGTQFDRGFLSPYFINSPEKQRVVLEDV
                     LVLLHDKKISAIRELLPLLEHAAKVGKPLLVVAEDIEGEALATLVVNTLRGVIKSCAV
                     KAPGFGDRRKALLEDLAVLTGARVVAEEAGLTLEKAEPSVLGRARRAEIDKDDTTLIG
                     GAGDPKAIDARVAQIKQQVTEATSDYDKEKLQERAAKLAGGVALIRVGAATETEMKER
                     KSRTEDALHATRAAVEEGVLPGGGVALLRARHCLAGRHGANGDQDAGVRIVLRALEEP
                     LRQIVRNAGDEPAVVVQRVIDGQGNFGYNAASGEYGDLVQMGVLDPCKVTRTALQNAA
                     SIAGLILTTDCMIARAPERPAAEGAEAAADAM"
     gene            complement(5222..5554)
                     /locus_tag="AC16O9_04590"
     CDS             complement(5222..5554)
                     /locus_tag="AC16O9_04590"
                     /inference="COORDINATES: protein motif:HMM:NF020918.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR6515928.1"
                     /translation="MAVPTDKTMLEAWKRQLDAAMRLTEVVIEGSQRMREMQIEAAAA
                     AHADAVATQKALAGASSPDELMRIQTEWLAANQRKSMQYWKDLYETAAETNTQLMSCL
                     RDSRPKSS"
     gene            complement(5723..6451)
                     /locus_tag="AC16O9_04595"
     CDS             complement(5723..6451)
                     /locus_tag="AC16O9_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6515929.1"
                     /translation="MEAGRRTHVAYGLLVAILAIAAGALFFAYQERGEEGARLAAAHR
                     ALSSERDALSSERNTLAGEREALKDEVKAAREQARVLEAELKSQRQKSAELAAEKEKL
                     SQGLTETQREAAAAAAARAELSRELQAKEQTLEQAKARSAELNKAYESLLRDKSQLAA
                     SDAAHRAELERTRKSFEEVQALIARLTGARGIYTVQDTDSLSTIAAFFYRDGNRWPDI
                     FKANAHLIDHPDLIYPEQVLIVPK"
     gene            complement(6465..9611)
                     /locus_tag="AC16O9_04600"
     CDS             complement(6465..9611)
                     /locus_tag="AC16O9_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6515930.1"
                     /translation="MGVSGRIARFFQDSQLTPLLALVAFLLGAFAVAVTPREEEPQIN
                     VTMANVLVPFPGAAARDVEALVATPAEQVLAQMSGVEHVFSVSRPGMAVLTVQFEVGV
                     ARTEALVRLYNTIQSNRDWLSPHLGVGEPVVKPKGIDDVPVVTLTLWRESGEAGALEL
                     ERVAHAAEAELKRVPGTREVQTLGGPGRAVRVLMDADRMNAHGVSPLDLANALQLANV
                     SLPAGSVIRENRETVVETNNYLTSGADVAALVVGTHDNRPVYVSDVAQVVDGAPPPAR
                     YVWTGTAKGEFPAVTLQVTKKPGENAVEVAERVLARAAELRGTVIPDDVQVSVTRDYG
                     ATANDKAQQLIRKLLFATLSVIVLVFAALGWREALIVGVAVLLTLAATLFASWAWGFT
                     LNRVSLFALIFSIGILVDDAIVVVENIHRRRGLEKRALADVIPLAVDEVGGPTILATF
                     TVIAALLPMAFVTGLMGPYMSPIPINASMGMLISLAIAFTLTPWLALKFFGAAAHGRE
                     SDRLERASRRILGPFVASKGWRRALLAAMLVLIAASVALAGVKLVVLKMLPFDNKSEF
                     QVVVDMPAGTPLEVTAGALHELGAHLATVPEVTDYQAYAGLSAPINFNGLVRQYYLRA
                     APEQGDLQVNLAPKEARSRKSHDIAQHVRPALEDIGRRWNANVKVVEVPPGPPVLSPI
                     VAEIYGPDEAGRIRVAKEVRAALAATPDVVGIDDSVEDPAPRIRLAVDQGKAALAGVA
                     RRDVAEAIRIGLAGADVTPLHDGQSKYEVPVRIALPAERQGGLDELLKLAVRAPSGAL
                     VPLSELVTPIADARDRPIYHKDLLPVVYVVADQAGPVDSPLYGMFAARAKLPEIDEYF
                     IAQPSDPYRSYALKWDGEWQVTYETFRDMGLAYGVGLVLIYLLVVAQFRSYLTPLVIM
                     APIPLTVIGVMPGHALLGANFTATSMIGMIALAGIIVRNSILLVDFVNLKLAEGMPLE
                     EAVIQSAAARAKPIALTGLAAMLGAAFILDDPIFNGLAISLIFGILVSTVLTLVVIPV
                     LYYVTRRGGSAGLNAAAV"
     gene            complement(9613..10659)
                     /locus_tag="AC16O9_04605"
     CDS             complement(9613..10659)
                     /locus_tag="AC16O9_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6515931.1"
                     /translation="MGRLTALVLAGLLAACGEAPAPRPQARPAVEVPTAVVGIRDVEL
                     TTSAEAVIEAVRQSTVSAQIAGRIVELNFDVGDFVNKGAVIARIDERAVAQAAEASEA
                     QVREAQAALGNARAGYERSKQLHAQKFISQAALEQAESAYKAAQARVSALLAGAGAAA
                     TERSFATIVAPYSGVVSARHVELGEMATPGRPLMTGFDPSTLRVVATVPQAQVAQIRA
                     LGRARIEVPSVGRWVDVKSMTLVPAADPRTHTTRIRLELPADVRGVYPGVYARAHFVT
                     GSEPRLLVPRAAVLRRSEVTAVYVIDGQGRALLRQVRLGAAGDETSVEVLAGLKPGER
                     VALEPIKAGMAPGA"
     gene            complement(10647..11072)
                     /gene="trxC"
                     /locus_tag="AC16O9_04610"
     CDS             complement(10647..11072)
                     /gene="trxC"
                     /locus_tag="AC16O9_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HIR6515932.1"
                     /translation="MSEFVQVACPACLAANRVPAARAGEDPKCGKCGAALLDGAPAEL
                     REDQFEAFVQRTGLPVLADFWAPWCGPCRSMAPHFERAARDLKDRVRLVKVNTEDAPG
                     LAQRYGIRAIPTLVLFRDGAEVKRASGAMDAGALARWAV"
     gene            complement(11069..11275)
                     /locus_tag="AC16O9_04615"
     CDS             complement(11069..11275)
                     /locus_tag="AC16O9_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR6515933.1"
                     /translation="MTVERVVRIFAGAFVLISLVLGLWVNPNFFWLTAFVGANLFQSG
                     FTGLCPLASILKRLGLREAAGVRA"
     gene            11428..11634
                     /locus_tag="AC16O9_04620"
     CDS             11428..11634
                     /locus_tag="AC16O9_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR6515934.1"
                     /translation="MKVNVGSVERILRVVVGVALLALLFVFEGSARWLGLIGLVPLAT
                     GLFGVCPAYTLFGLSTCPVESKRA"
     gene            11662..12336
                     /locus_tag="AC16O9_04625"
     CDS             11662..12336
                     /locus_tag="AC16O9_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6515935.1"
                     /translation="MTAPGRDILQALVERFSALAGLAPARLERLLAEAQLLRVPAGTI
                     LFDADQPCRGFPLVLEGAVRVQMNAPSGREIMLYRVGPGEGCILSGGCLLGHSDYAAR
                     GIVEEDVTLLNLPPAAFNALLLEHEPFRRFVFGMYGERLAEVMQLVEEVAFRRLDERL
                     AQLLIHRGPVIEATHQKLADELGSVREIVSRLLRSFEQRGWVKLERERITLTDPKSLA
                     ALGAPA"
     gene            complement(12333..13640)
                     /locus_tag="AC16O9_04630"
     CDS             complement(12333..13640)
                     /locus_tag="AC16O9_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR6515936.1"
                     /translation="MQLSFLGAAGEVTGSCYLVDTGEAKFLIDCGMRQGGRQVRALNR
                     RFAFAPRDIAFVLLSHAHIDHSGLVPRLVAQGFGGPVYATRATCELLGVMLPDSAHLQ
                     EKEAEREGAEPLYTVQQAQQSLKRLQAVDYDEDLRPYAAVRCRFRDAGHILGSAILEI
                     WLGRKKLVFSGDLGQPGRPVVEDPTPIREADVLLVESTYGNRNHKNLAQTLDEFAFAL
                     NDTLASKKGNVVIPSFAVGRTQDLLSIMGDLQRAGRLPKLNAFVDSPMASAATRITLR
                     HAALPALDRVRFTESTEDSMKINAIRSGAVIISASGMCEGGRIKHHLRHNLPRKECAV
                     VFVGFQAAGTLGRRLVDGARRVRIHGEDVPVRAKVFTINGLSAHADRDALLDWMGKFR
                     RAPRQCFVVHGEPLSAHALRDGIRAELGWRAEVPEQGQTVDLA"
     gene            13717..16254
                     /gene="glgP"
                     /locus_tag="AC16O9_04635"
     CDS             13717..16254
                     /gene="glgP"
                     /locus_tag="AC16O9_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR6515937.1"
                     /translation="MRGTPYRLDVRPRLPERIGRLAELAGNLWYSWHRATRSLFAQLD
                     PGLWTEINHGPKAFLLNIDQQRLDDAARDPAFLAAYDATLAAYDAYHAQSATDGRFAP
                     GELVAYFCAEFGLHESMPIYSGGLGILAGDHCKTASDLRLPFVGVGLLYRQGYFEQAL
                     DSDGRQQARYVDNDFELLPVEPAVRADGGEVRVQVELPGGPLHIKVWRARIGHVSLYL
                     LDTDLEENREANRDIVHRLYEGSRGVRLEQEIVLGVGGARALAALGIEPTCWHMNEGH
                     PAFLSLERLRRLVAEGVPFAAALDAVAANSVFTTHTAVPAGHDAFPVDVVREYAHSVL
                     PELRPHLDEVLELGRSSGNGNFNMTALAVRATRARNCVSRIHTGVSQRMLAALWPEIE
                     PEENPLVSITNGVHLPTYLSDAWHELFDRCLGADWPARMYQPECWNAVRAIPDAEFWN
                     VHQLLKAQMLHIVRFRVARQHARNQGSEAHLERMLRLADPARPDVLTIGFGRRFATYK
                     RATLLFQDLDWLRQIICVHGRSVVFLFAGKAHPADEPGQALMRRIVEVSRLPEFESRI
                     LLVEGYDLRLARRLVAGVDVWLNNPLYPLEACGTSGMKAAMNGVLNLSVTDGWWREGY
                     DGRNGWAIKPASPALDATRRDAEEARTLYELLQDQVVPLYYARNDAGFSPGWVALAKE
                     SMATVAPRFNSMRMLEEYVGQLYLPAAARGQRLAAGGHAAARELAEWRAKVRAAWPGV
                     VLRRAGEPPMRAAHGEGVRIAVAVRLNGLAPGDLALELVLQRPAAQSPAAHYRFAPHG
                     PLAESGEHLYELEFAPEHNGRLEYRLRAYPSHELLAHPFETGLMIWL"
     gene            16403..17878
                     /gene="glgA"
                     /locus_tag="AC16O9_04640"
     CDS             16403..17878
                     /gene="glgA"
                     /locus_tag="AC16O9_04640"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR6515938.1"
                     /translation="MPVGPAPRVLLVTPEVFPLAKTGGLADVCAALPRALARRGADVR
                     FLVPAYTRALDRVHAPRVAARLGPLLGVASAQLIQGAMPGSGLPVWLLDCPELYRRPG
                     TLYQDPDGKDWPDNWQRFGLLAHAAARIALGEAQLDWQPEVVHCHDWHTGLAPYLIGR
                     AAAPRPRTLFTVHNAAFQGNFPAEHAPHLGLPPEVLSPEAMEFWGQLSFLKAGVRYAD
                     RVNTVSPTYARELFTAEFGYGLEGLFRHRGSDFTGILNGIDTELWDPAADDGIAARFS
                     ADAPQGKADCKTALQAALHLARDPQAPLVAFASRITWQKMADVTLERLPALLAANSRL
                     QFALLGTGERALERGFLALAPHYPGRVSVRIAYTEDEERRLQAGADVLLHGSRYEPCG
                     LAHKIAMRYGALPVVRRTGGLADTVREGVNGFMFESTAGEDLQAAVARCLATYAQRPD
                     AWRAMRDHAMCGDYSWEQPALEYLALYRAAPVIASAGSTSG"
     gene            complement(17847..18122)
                     /locus_tag="AC16O9_04645"
     CDS             complement(17847..18122)
                     /locus_tag="AC16O9_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515939.1"
                     /translation="MKRLCLALAFASLPAAAQDPNRGELVYKTQCGGCHYERLHERER
                     SKSRVRSLADLRDQVVLRAGQTGRPFTLEDLEDVAEYLNRSYYRLTQ"
     gene            complement(18119..>18328)
                     /locus_tag="AC16O9_04650"
     CDS             complement(18119..>18328)
                     /locus_tag="AC16O9_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515940.1"
                     /translation="LWPSFIVGGIGEVIFFTVFDPQELYLFGEPTTLSRLGVYSIGFF
                     LCWAFAASSSALTCFLQRGPKEINR"
BASE COUNT         2656 a         6601 c         6306 g         2765 t
ORIGIN      
        1 gggcgtgccg gggcggcgca tcgccgatcc cgcggacatc gcaccggcgc tgcgcgaggc
       61 gctcgccgcc ggcggcccgc gcctgatcga agtcatggtc gccgacggct tcggcacctg
      121 aactagcgcc gcagcacgca cttgtcgtag agcgtcggga acgccccggc cgggtcgtac
      181 ttgcgcttga gcgcggcgta ggcctcgccg ccgtagaggc ggtggaactc gtcctcggga
      241 aagaagctct ccgagtacag cgacttgatg ccgcccagct cggtcacttt gcgctcgatg
      301 agccggttga agtgccccgg ggcgtgcgct tctcgcgtgc gcttcacgtc ccagaagccg
      361 aagttcacga cccaccccgg cgccatcggg tagagggtga agcgcccggc gtgcggcgcc
      421 gggccgatgg gacagatcca ctgcggccac aggccgatct cccggcggta gaactcgaag
      481 aactcggcgg cgcgctcgag cgggatgtcg acgtcctgga tcaccgactc ggaatgcaag
      541 ccgagcagac gctcgaattt cttcgtcagg ccgacgcggc tgttccagcg catcaccttc
      601 gtgtaggtgc gcgagcccag gcgccgccgg ccgtagacga agcggcgcac gagcggctgc
      661 tgcgcgagca cgttcttcga gcaccagaac cagtcggtgt cccagcgcca caggtagtcg
      721 tgcaccgtga gccagtcctc gcgcttctcg gcgatcgaac ggtaatagat gtgctcgaag
      781 gtgtagtcgc tcgtatgggg cgccttggcg gcgaagcgcc cgagggtgag gaacatcttt
      841 cgcggcgcga acagcgtgcc gtcgacgaaa tccgcgtcgc ccgcgcgcag gcgctcgccg
      901 agcgcctcga acaagcgcgc cgggtcggcg taaggctcgt gcgtcaggtg cacgtaaggc
      961 tgcaccggca ccactttcgc cttcacgcgc agcgcgtagc ccagcgtgcc gtaggaattg
     1021 ggaaagccgt aataaaggtc ggcgtgctcg ttgtcgggcg cggcgagcac gatcgagccg
     1081 tcgccgagca gcacctcgat ctcgtgcacg gtgtcgtgca ccaatccgtg gcggtgcgac
     1141 gaggactcga tgcccacccc cgccaccgcg ccgccgagcg tgatggtctt gagctgcggc
     1201 accaccgccg gcatcacccc gcgcaccagg caaccgcgca ccaggtcttc gtaggaggcc
     1261 atgccctcgg cgtccaccca gccggccgcc gcgtcgacct ccagcacgtc gcggaatgcg
     1321 cgcacgtcga ggcgccggcg cgtgccgccg gcgcggtcgc gaaacaggtt cgaggtgccc
     1381 ttggcgagcg cgaccgggcc ggcgccgcgg ctaagctgcg cgctgagcgc ctgcgtcttc
     1441 gcggtgtgcg cgctgctcgt agaggaactc ccggctcatg gggtaattct tgcgggtgat
     1501 gttgcccttc gagaacacga tctgaaagag gtgcgtgtag ccggcggccg agcggaacat
     1561 ctccgcgcag ccgtacaggt acacgcgcca gacgcggcga aagcgctcgt cgaacttctt
     1621 cgggtcgagc gcctggatct gcggccagtg gcggtcgaag cgctcgcccc aggcatcgag
     1681 cgtcagcgcg tagtgccggc gcaggttctc gatgtccacc acctcgagtc cggcgcgctc
     1741 catggcggcg atggtgtgcg cgaggctcgg gatccagccg cccgggaaca cgtgcttgcg
     1801 gatgaagaac tcggtctgga agtgcccgac gtggccgatg aagtgcagca tgccgagccc
     1861 cccgggcttg aggaacgcgg cgtgcgcgcg caccacctcc tccagcgcgt cgcgcccggc
     1921 gtgctcgagc gtgccgatcg acaccacctt gtcgtactgg cggcgcgtgt cgcggaaatc
     1981 ggtgtcgatg acgtcaagtg cgccgcccag tccgcggcgc gcgatctgcg cacgcaccat
     2041 ctcgacctgc gaggtggtgt tgttgatgcc ggtgaccttg acgccgaact tgtcgtgcgc
     2101 gcggaacatg aagccgccga agccgctgcc gatgtcgacc accgtctcgc ccggtgcgag
     2161 gcgcaccttc cgcgccacgt ggtcgatctt gttctgctgc gcctgctcca gcgtcttcgt
     2221 gccttccttc cagtaggcgc aggtgtacat catgtgcggg tcgtcgagcc agtagcggta
     2281 gaactcggtg cccagcgcgt agtggaattc ggcgttcttc tttgcctgtt gccagctgcg
     2341 gttggagaac ctgaactcgt gccagcggtt gcgcaagccg acgagcgggt tagcgccggc
     2401 gacgccgccc accagaccca ccgccatggc cgcggcgagg ctaccctcga tgtcgaggtc
     2461 gccgtcgaag tacgattcga gcaggccgat gtggccgaag gcggcgatgc gcgcataggc
     2521 cgcggcgcgg tggaatacga ccgtgaaggc cggcgcaccg tcgcggctgc gccactcgcc
     2581 gccgcccgcg aagcgcacgg cgaagggagc gctggagcgc tccccggcct tgcgcagcac
     2641 cgattcgacg acggatctca cggccggcca ttctaccggc cggcgcggct tcagtgcgac
     2701 atcagcaccg gcacggtcat cgcctcgagc agggtgcgcg tggcgccgcc gagcaccatc
     2761 tcgcgcacgc gcgcgtgccc gtaggcgccc atcacgatca ggtccgcgcc ggtgtccgcg
     2821 gcacgcgaca ggatctgcgc gccgacgccg tagcccgcgc cgctctggcg cgccaccgtc
     2881 accttgacgc cgtggcgcgc gaggtacagc cccacgtcgg cccccggctg ctgaccgtgg
     2941 ctgttcaggc gcttctccgg atcgaaggcg cacacctcga ccgcctcggc gcggcgcagg
     3001 agcggcagcg cgtcggtgac cgcccgtgcc gcctcgcgcc cgccatccca ggcgacgagc
     3061 acacgcttgc cgagcatggg gaagcgcccc gcgtaaggca cgaacagcac cggccggccg
     3121 gcggagaaca ccagctcatc ggcgaggccg cgcaggtcgc cttcgtatgt agcctcgggc
     3181 tggcccgcga ccaccaggtc ggcgtagcgc gccgacaagc gcatcgccga cgcggcgtcg
     3241 gactgcgtgc tgcgccactc ggtgcgcccg ccattgcgcc gcgcgacgtc gcggaagcgc
     3301 cgctccgcct ctgccgcgac ctcggcgttg cgcttctcga gcatgtcgcg cagcagcggg
     3361 ccgccctcgg cgagcgcgta agacggcagc ctctgcaagc cggaggcgaa caagccgacg
     3421 atgtgggcgt cgaactcgtc ggcaaggctg caggcgaggc cgagccgctc ggtgctgcgc
     3481 gggtggtggt cgaagtgcac gagaatggtc ttgtacgtca tggttgcctc tgggtcccag
     3541 ccctcatgat gcgccgcgtg cgccggcggg gcttgacgaa agtcaactac atcgcgtccg
     3601 ccgcggcctc ggcgccctcg gccgccggcc tctcgggcgc acgggcgatc atgcagtcgg
     3661 tggtcaggat gagccccgca atggaagccg cattctgcag cgcggtgcgc gtcaccttgc
     3721 agggatcgag cacgcccatc tgcaccaggt cgccgtactc gccgctcgcc gcgttgtagc
     3781 cgaaattgcc ttggccgtcg atcacacgct gcacgacgac cgcgggctcg tcgcccgcgt
     3841 tacggacgat ctggcgcagc ggctcctcca gcgcgcgcag cacgatgcgc acgcccgcgt
     3901 cctggtcgcc gttcgcaccg tgccgccccg cgaggcaatg gcgcgcgcgc agcagcgcca
     3961 cgccgccgcc cggcagcaca ccttcctcga ccgcggcgcg cgtcgcgtgc agcgcatcct
     4021 cggtgcgcga cttcctttcc ttcatctcgg tctcggtggc cgcgccgacg cggatcagcg
     4081 ccaccccgcc cgcgagcttg gccgcgcgtt cctgcagctt ttccttgtcg taatcgctcg
     4141 tcgcttccgt cacctgctgc ttgatctgcg ccacgcgcgc gtcgatcgcc ttggggtcgc
     4201 ccgcgccgcc gatcagcgtc gtgtcgtcct tgtcgatctc cgcgcgccgc gcgcgcccga
     4261 gcaccgacgg ctcggccttt tccagcgtca ggccggcctc ttcggcgacc acccgagcgc
     4321 cggtgagcac ggcaaggtcc tccagcaacg ccttcctgcg gtcgccgaag cccggcgcct
     4381 tcacggcgca ggacttgatc accccgcgca gcgtgttgac caccagcgtg gcgagcgcct
     4441 cgccctcgat gtcctcggcg accaccagca gcggcttgcc caccttggcc gcgtgctcga
     4501 gcagcggcag cagctcgcgg atcgcggaga tcttcttgtc gtgcaggagg accagcacgt
     4561 cctcgagcac cacgcgctgc ttttccggac tgttgatgaa gtagggcgaa agaaagccgc
     4621 ggtcgaactg cgtgccctcg accacctcga gctcgttggc gaggcccttg ccgtcctcga
     4681 cggtgatcac gccctcgcgc ccgaccttct ccatcgcttc ggcgatcatc tcgccgatgg
     4741 aagcgtcgtt gttggcggag atggcgccga cctgcgcgat ttccttgcgc gtcgcgcagg
     4801 gcttggccat gcgcttgagc tcgcccacca cgccctcgac cgccaggtcg atgccgcgct
     4861 tcaggtccat cgggttcatg ccggcggcga cgtacttcat gccctcggtg accacggccg
     4921 cggcaagcag cgtcgccgtg gtggtgccgt cgcccgccac ctccgacgtc ttgctcgcca
     4981 cctcgcgcac catctgcgcg cccatgttct cgaacgggtc ggcgagctcg atctccctgg
     5041 cgacgatcac gcccgagttg atgacgatgg gcgcgcccca ggacttctcc agcaccacgg
     5101 tgcgcgcctt cggccccagg gtcacgcgca ccgcgtcggc gagcgtattc atgcccttca
     5161 ccagccgggc atgggcggtt tcgccgaaca gcagttgctt cgcgggcacc gcgcgtctcc
     5221 gtcagctcga cttcggcctc gagtcgcgca ggcagctcat cagctgcgtg ttggtctcgg
     5281 ccgcggtctc gtaaaggtct ttccagtact gcatggactt gcgctggttg gcggccagcc
     5341 actcggtctg gatgcgcatc agctcgtcgg ggctggaagc gccggcgagc gccttctgcg
     5401 tggcaactgc gtcggcgtgc gctgcggcgg cggcctcgat ctgcatctcg cgcatgcgct
     5461 gcgagccctc gatcaccacc tcggtgagcc gcatcgccgc gtcgagctgg cgtttccacg
     5521 cctcgagcat ggtcttgtcg gtcggcaccg ccatggcgac ttccaccgga atccggaatc
     5581 aagatacacg cgcgcccggt ctcgggcttg atccacgtca acgaccgcgc tgcgcgctgc
     5641 agcagcttca tacccgcacg tcgcgaaagc tggccgccag ggccaaaccg gcggtccggc
     5701 agcccgttga cctacgccaa tgtcacttcg gcacgatcag cacttgctcg ggatagatca
     5761 ggtccggatg atcgatgagg tgcgcgttgg ccttgaagat gtccggccag cggttgccgt
     5821 cgcggtaaaa gaaggcggcg atcgtcgaca gcgaatccgt gtcctgtacc gtgtagatgc
     5881 cgcgcgcgcc ggtcagccgt gcgatcagcg cctggacctc ctcgaacgac ttgcgcgtgc
     5941 gctccagctc cgcgcggtgc gccgcatccg acgcggcgag ctgcgacttg tccctcagca
     6001 ggctttcgta ggccttgttc agctctgccg agcgcgcctt ggcctgctcc agggtctgct
     6061 ccttcgcctg cagctcgcgc gacagttccg cgcgcgccgc ggccgcggcg gcggcctcgc
     6121 gctgcgtctc ggtcagtccc tgcgagagct tttccttctc agcggcgagc tcggcgctct
     6181 tctgccgctg cgatttcaac tccgcctcga gcacgcgcgc ttgctcgcgc gccgccttca
     6241 cttcgtcctt cagcgcctcg cgctcgccgg cgagggtatt tcgctcgctg ctcagggcat
     6301 cgcgctcact gctcagggcg cggtgcgcgg cggccagacg tgcgccctcc tcgccccgct
     6361 cctggtaggc gaagaacagc gccccggcgg caattgccag tatcgccacc agcaacccgt
     6421 aggcgacatg cgtgcgccga ccggcctcca tggcgctatg accgtcagac cgcggcggcg
     6481 ttcagcccgg ctgatcctcc gcggcgcgtg acgtaataga gcacgggaat caccaccagc
     6541 gtcagcacag tggagacaag aatcccaaaa atcagcgaga tagcgaggcc gttgaagatc
     6601 gggtcgtcga ggatgaacgc cgcccccagc atcgcggcga gcccggtgag cgcgatcggc
     6661 ttggcgcgcg ccgcagccga ctggatcacc gcttcttcca gcggcatgcc ctcggcgagc
     6721 ttcaagttga cgaagtccac cagcaggatg gaattgcgca cgatgatccc ggcgagcgcg
     6781 atcatgccga tcatcgacgt ggcggtgaag ttcgccccca gcagcgcgtg ccccggcatc
     6841 acgccgatca cggtgagcgg gatcggcgcc atgatgacga gcggcgtgag gtacgagcgg
     6901 aactgcgcca ccaccagcag gtagatgagg accagcccca ccccatacgc gaggcccatg
     6961 tcgcggaagg tctcgtaggt cacctgccac tcgccgtccc acttcagcgc atagctgcgg
     7021 tagggatcgg agggctgcgc gatgaagtac tcgtcgatct cgggcagctt cgcgcgcgcg
     7081 gcaaacatgc cgtagagcgg actgtccacg gggcccgcct ggtcggcgac cacgtacacc
     7141 accggcagca gatccttgtg gtagatcggc cggtcgcgcg cgtcggcgat cggcgtgacg
     7201 agctcggaaa gcggcaccag cgcgccggag ggcgcgcgta ccgcgagctt gagcagctcg
     7261 tcgagcccgc cctggcgttc agcgggcagc gcgatcctca ccggcacttc gtacttcgac
     7321 tgcccgtcgt gcaagggggt gacgtcggca ccggcgaggc cgatgcggat cgcctcggcc
     7381 acgtcgcggc gggcgacgcc cgcgagcgcc gccttgccct ggtccaccgc gagccggatg
     7441 cgtggcgccg gatcctccac cgagtcgtcg atgccgacga cgtcaggggt ggcggcgagc
     7501 gcggcgcgca cctctttcgc cacgcggata cggcccgcct cgtcggggcc gtagatctcg
     7561 gcgacgatcg gcgagagcac cggcggtccg gggggcacct cgaccacctt cacgttcgcg
     7621 ttccagcgcc ggccgatgtc ctcgagcgcc gggcgcacgt gctgcgcgat gtcgtggctc
     7681 ttgcggctgc gtgcctcctt cggcgcgagg ttcacctgca ggtcgccctg ctcgggcgcc
     7741 gcgcgcaggt agtactggcg caccaggccg ttgaaattga tcggcgcgga cagcccggcg
     7801 taggcctggt agtccgtgac ctcgggcacc gtggcgagat gcgcgccgag ctcgtgcagc
     7861 gctcccgcgg tgacttcaag cggcgtgccc gccggcatgt cgaccaccac ctggaactcc
     7921 gacttgttgt cgaagggcag catcttcagc accaccagct tcacgcccgc gagcgcgacc
     7981 gaggcggcga tcagcaccag catcgcggcg agcagcgcgc gccgccagcc tttcgaggcg
     8041 acgaacgggc cgaggatgcg gcgcgaggcg cgctcgaggc ggtcagactc ccggccgtgc
     8101 gctgcggcgc cgaagaactt gagcgcgagc cacggcgtca gcgtgaaggc gatggcgagc
     8161 gagatcagca tccccatgct ggcgttgatc gggatcgggc tcatgtacgg gcccatcagc
     8221 ccggtgacga aggccatcgg cagcagcgcc gcgatcaccg tgaaggtggc gaggatcgtc
     8281 ggccccccga cctcgtcgac ggcgagcggg atgacgtcgg caagcgcgcg tttttccagc
     8341 cccctgcgcc ggtggatgtt ctccaccacc acgatggcgt cgtcgaccag gatgccgatg
     8401 gaaaagatga gcgcaaagag cgacacccgg ttcagcgtga agccccaggc ccacgaggcg
     8461 aagagcgtgg ccgccagcgt aagcagcacg gcgacgccga cgatcagcgc ttcacgccag
     8521 ccgagcgcgg cgaacacgag cacgatcacc gagagcgtgg cgaagaggag cttgcggatc
     8581 agctgctgcg ccttgtcgtt cgccgtggcg ccgtagtcgc gcgtcacgct gacctgcaca
     8641 tcgtcgggga tcaccgtgcc gcgcagctcc gcggcgcgcg ccagcacgcg ctcggcaacc
     8701 tctaccgcgt tctcgccggg cttcttggtg acctgcagcg tgaccgcggg aaactcgcct
     8761 ttggcggtgc cggtccagac gtagcgcgcg ggcggcggcg cgccgtccac gacctgcgcg
     8821 acgtcgctca cgtacaccgg ccggttgtcg tgcgtgccca ccaccagcgc ggccacgtcg
     8881 gcgccgctcg tcagatagtt gttggtctcg accaccgtct cgcggttctc gcggatcacg
     8941 ctcccggccg gcagcgagac attggcgagc tgcagcgcgt tggcgaggtc gagcggcgag
     9001 acgccgtgcg cgttcatgcg gtcggcgtcc atcagcacgc gcaccgcgcg tccggggccg
     9061 ccgagcgtct gcacttcgcg cgtgccgggc acgcgcttca actcggcttc cgccgcgtgc
     9121 gccacgcgct cgagctcgag cgcccccgct tccccgctct cgcgccacag cgtcagcgtc
     9181 accaccggca cgtcgtcgat gcccttgggc ttgaccaccg gctcgccgac gcccaggtgc
     9241 ggcgagagcc agtcgcggtt cgactggatc gtgttgtaga ggcgcaccag ggcctcggtg
     9301 cgcgccacgc cgacctcgaa ctgcacggtg agcaccgcca tgccggggcg cgacaccgag
     9361 aacacgtgct cgacgccgct catctgcgcg agcacctgct cggcgggcgt cgccaccagc
     9421 gcctcgacgt cgcgcgcggc cgcgccgggg aacggcacca gcacgttcgc catggtgacg
     9481 ttgatctgcg gctcctcctc gcgcggggtc accgccacag cgaaggcacc gagcaggaac
     9541 gccaccagcg cgagcagcgg cgtgagctgc gaatcctgga agaagcgcgc gatgcgcccg
     9601 gagacgccca cgtcaggccc cgggcgccat gcccgccttg atcggctcca gcgccacgcg
     9661 ctcgccgggc ttcaggcccg cgagcacttc gaccgaggtc tcgtcgcccg cggcgcccag
     9721 gcgcacctgg cgcagcagcg cgcggccctg gccgtcgatc acgtacaccg cggtgacctc
     9781 gcttcgcctc agcaccgcgg cgcgcggcac cagcagtcgc ggctcgctgc cggtgacaaa
     9841 gtgcgcgcgc gcgtagacac ccgggtacac gccgcgcaca tccgccggca gctcgaggcg
     9901 gatgcgcgtg gtgtgcgtgc gcgggtcggc cgccggcacc agggtcatgg acttgacgtc
     9961 gacccagcgg ccgaccgagg gcacttcgat gcgcgcgcgg ccaagggcgc ggatctgcgc
    10021 cacctgcgcc tgcggcacgg tggccaccac gcgcagcgtc gaggggtcga atcccgtcat
    10081 gagcggccgc ccgggcgtcg ccatctcgcc gagctcgacg tggcgtgccg agaccacgcc
    10141 gctgtagggc gcgacgatgg tggcgaagct gcgttcagtg gccgccgccc cggcgcccgc
    10201 gagcagcgcg ctgacgcgcg cctgcgccgc cttgtaggcg gactcggcct gctcgagcgc
    10261 cgcctggctg atgaacttct gcgcatggag ctgcttcgag cgctcgtagc ccgcgcgcgc
    10321 gttgccgagc gcggcctgcg cctcgcgcac ctgcgcctcg ctcgcctcgg cggcctgtgc
    10381 caccgcgcgc tcgtcgatgc gcgcgatcac cgcgcccttg ttcacgaagt cgccgacgtc
    10441 gaaattgagc tcgacgatgc ggcccgcgat ctgcgccgag accgtcgact ggcgcacggc
    10501 ctcgatcacg gcttcggccg aggtcgtgag ctcgacgtcg cggatcccga ctaccgcggt
    10561 cggcacttcc accgcggggc gcgcctgcgg cctgggcgcc ggcgcctcgc cgcacgcggc
    10621 gagcagcccc gcgaggacga gcgcggtcag acggcccatc gcgccagcgc tccggcatcc
    10681 atggcgcccg aggcgcgctt cacctcggcg ccgtccctga acagcaccag cgtcgggatc
    10741 gcgcggatgc cgtaacgttg cgccaggccg ggcgcgtcct cggtattcac ctttaccagc
    10801 cgcacgcggt ccttcaggtc gcgcgccgcg cgctcgaaat gcggcgccat cgaacggcac
    10861 gggccgcacc agggcgccca gaaatcggcg agcaccggga gcccggtgcg ctgcacgaac
    10921 gcctcgaact ggtcctcgcg cagctcggcg ggcgcgccgt cgaggagtgc cgccccgcac
    10981 ttgccgcact tcggatcctc gcccgcccgc gccgcgggga cgcggttggc ggcaaggcag
    11041 gccgggcagg cgacctgcac gaattcgctc acgcgcggac tcctgcggcc tcacgcaggc
    11101 ccagccgctt caggatcgaa gcgagcgggc acaggccggt aaagccgctc tggaacaggt
    11161 tggcgccgac gaacgcggtc agccagaaaa agttcgggtt gacccacagt ccaagcacga
    11221 gcgaaatcag cacgaatgcc cccgcaaaga ttctcaccac gcgttccacc gtcatgttga
    11281 cctccgtcaa agatatgacc cgcagcctac cgggcggggg cgcgcgcatc tgtgaccgag
    11341 gttccacagc gctcgggaac ctgggtcaca gaccgcgcgg tgccccgggc gcgacactgc
    11401 agcgtcgata accttaggag agccccgatg aaagtgaacg ttggcagcgt ggaacggatc
    11461 ctgcgcgtgg tggtgggcgt cgccctgctc gccctgctct tcgtgttcga aggcagcgcg
    11521 cgctggctcg ggctcatcgg cctcgtgccg ctcgccaccg gcctgttcgg cgtgtgcccg
    11581 gcgtatacgc tgtttggcct gagcacctgc ccggtggagt cgaagcgcgc ctgagctgcg
    11641 cactaggcta gactcggccg ggtgaccgca cccggccgag acattttgca ggcgctcgtc
    11701 gagcgctttt cggcgctcgc gggcctcgcg cccgcgcggc tcgagcgcct gctcgccgaa
    11761 gcgcagctcc tccgcgtccc ggcgggcacg atcctgttcg acgccgacca gccctgccgc
    11821 ggcttcccgc tcgttctgga gggcgcggtg cgcgtgcaga tgaacgcgcc gagcggccgc
    11881 gagatcatgc tgtatcgcgt cggaccgggc gagggctgca tcctgtccgg cgggtgcctc
    11941 ctcggccatt cggactacgc cgcgcgcggc atcgtcgagg aggacgtgac gctgctcaac
    12001 ctgccgccgg cggcgttcaa cgcgctgctg ctcgagcacg agccgttccg gcgcttcgtc
    12061 ttcggcatgt acggcgagcg cctcgccgaa gtgatgcagc tcgtcgagga ggtcgcgttc
    12121 cgccgcctcg acgagcgcct ggcacagctg ctgatccacc gcggcccggt gatcgaagcc
    12181 acgcaccaga agctcgccga cgagctcggc agcgtgcgcg agatcgtgag ccggctgctg
    12241 aggagcttcg agcagcgcgg ctgggtgaag ctcgagcgcg agcgcatcac gctcaccgac
    12301 ccgaagtcgc tcgccgcgct gggcgcgccc gcttaggcga ggtcgacggt ctgcccctgc
    12361 tcgggcacct cggcgcgcca gccgagctcc gcgcggatgc cgtcgcgcaa ggcgtgcgcg
    12421 gagagcggct cgccgtgcac cacgaagcac tgccgcggcg cgcggcggaa tttccccatc
    12481 cagtcgagca gcgcgtcgcg gtcggcatgc gccgacaggc cgttgatggt gaacaccttg
    12541 gcgcgcaccg gcacgtcctc gccatggatg cgcacgcgcc tggcgccgtc gacgaggcgc
    12601 cggccgagcg tgcccgcggc ctggaagccc acgaacacca ccgcacattc cttgcgcggc
    12661 aggttgtggc gcaggtggtg cttgatgcgc ccgccttcgc acatgccgct cgccgagatg
    12721 atcaccgcgc ccgagcggat ggcgttgatc ttcatcgagt cctcggtgct ctcggtgaat
    12781 cgcacccggt ccagcgcggg cagcgcggcg tggcgcagcg tgatgcgggt tgccgcgctc
    12841 gccatcggcg agtcgacgaa ggcgttcagc ttcggcaggc gccccgcgcg ctgcaggtcg
    12901 cccatgatgg aaagcaggtc ctgcgtacgc ccgaccgcga acgacgggat caccacgttg
    12961 ccctttttcg aggcgagcgt gtcgttcagc gcgaaggcga attcgtccag ggtctgcgcc
    13021 aggttcttgt gattgcggtt gccgtaggtc gattcgacca gcagcacgtc ggcttcgcgg
    13081 atcggcgtcg gatcctcgac caccgggcgc ccgggctggc cgaggtcgcc ggagaagacc
    13141 agcttcttgc ggccgagcca gatctcgagg atcgccgagc cgaggatgtg gccggcgtcg
    13201 cgaaagcggc agcgcaccgc ggcatagggc cgcaagtcct cgtcgtagtc caccgcctgc
    13261 aggcgcttca ggctctgctg cgcctgctgc acggtataga gcggctcagc gccctcgcgc
    13321 tcggcctcct tttcctgcaa gtgcgcgctg tcgggcagca tgacgccgag cagctcgcag
    13381 gtcgcgcgcg tggcatagac cgggccgccg aaaccctgcg caacgagccg cggcaccagg
    13441 cccgaatggt cgatgtgcgc gtgcgacagc agcacgaagg cgatgtcgcg cggcgcgaat
    13501 gcgaagcggc ggttcagcgc gcgcacctgg cgcccgccct ggcgcatgcc gcagtcgatg
    13561 aggaacttcg cctcgccggt gtcgaccagg tagcacgagc cggtgacctc gccggcggcg
    13621 ccaagaaagc tgagctgcat cgcccgcagc ttactgcagc gcccggcgca gcgcgccagt
    13681 tgcgctagac tgcgcccgaa gaccacactg cactccgtgc gcggaacccc ctaccgactc
    13741 gacgtccgtc cgcgccttcc cgagcgcatc ggccgtcttg ctgagctggc cggcaacctc
    13801 tggtacagct ggcaccgcgc cacgcgcagc ctgttcgccc agctcgaccc gggcctgtgg
    13861 accgagatca accacggccc caaggcgttc ctgctcaaca tcgatcagca gcggctcgac
    13921 gacgcggcgc gcgatccggc gttcctcgcc gcttacgacg ccacgctcgc cgcctacgac
    13981 gcctatcacg cgcaatcggc gaccgacggc cgcttcgcgc ccggcgagct ggtggcgtac
    14041 ttctgcgccg agttcgggct gcacgagagc atgccgatct actcgggcgg cctgggcatt
    14101 ctcgccggcg accactgcaa gacggcgagc gacctgcgcc tgccgttcgt cggcgtcggg
    14161 ctgctgtacc gacagggcta cttcgagcag gcgctcgaca gcgacggccg ccagcaggcg
    14221 cgctacgtcg acaacgattt cgagctgctg ccggtggagc cggcggtccg cgccgacggc
    14281 ggggaagtgc gcgtgcaggt ggagctgccg ggcggcccgc tgcacatcaa ggtgtggcgc
    14341 gcgcgcatcg ggcacgtgtc gctgtacctg ctcgacacgg acctcgagga aaaccgcgaa
    14401 gccaatcgcg acatcgtgca ccggctctac gagggcagcc gcggcgtgcg gctggagcag
    14461 gagatcgtgc tcggggtggg cggcgcgcgc gcgctcgcgg cgctcggcat cgagccgacc
    14521 tgctggcaca tgaacgaggg gcacccggcg ttcctgtcgc tcgaacgact gcgccgcctg
    14581 gtcgccgagg gcgtgccgtt cgcggcggcg ctcgacgccg tggcggcgaa ctcggtgttc
    14641 accacccaca ccgcggtgcc tgccggccac gacgccttcc ccgtcgacgt ggtgcgtgaa
    14701 tacgcgcaca gcgtgttgcc cgagctgcgg ccgcacctgg acgaggtgct ggagctcggc
    14761 cgctcctcgg gcaacggcaa cttcaacatg acggcgcttg cggtgcgcgc cacgcgcgca
    14821 cgtaactgcg tctcgcgcat ccacaccggg gtctcgcagc gcatgctcgc cgcgctgtgg
    14881 ccggagatcg agcccgagga gaacccgctg gtatcgatca ccaacggcgt gcacctgccg
    14941 acctacctgt cggacgcctg gcacgagctg ttcgaccgct gcctgggcgc ggactggccg
    15001 gcgcgcatgt accagcccga atgctggaac gccgtgcgcg ccattccgga cgcggagttc
    15061 tggaacgtgc accagctgct caaggcgcag atgctgcaca tcgtgcggtt tcgcgtggcg
    15121 cgccagcacg cccgcaacca gggctcggaa gcgcacctcg aacgcatgct gcgcctggcc
    15181 gaccccgcgc ggccggacgt gctcaccatc ggcttcgggc ggcgcttcgc cacctacaag
    15241 cgcgccacgc tcctgttcca ggacctcgac tggctgcgcc agatcatctg cgttcacggg
    15301 cgctcggtgg tattcctgtt cgcgggcaag gcgcatccgg cggacgagcc gggccaggca
    15361 ctgatgcgcc gcattgtcga ggtgtcgcgc ctgcccgagt tcgagtcgcg catcctcctg
    15421 gtcgagggct acgacctgcg cctcgcgcgc cgcctggtgg cgggcgtcga cgtctggctc
    15481 aacaacccgc tgtacccgct ggaagcctgc ggcacctcgg gcatgaaggc ggcgatgaac
    15541 ggcgtgctca acctgtcggt gaccgacggc tggtggcgcg agggctacga cgggcgtaac
    15601 ggctgggcga tcaagcccgc ctcgcccgcg ctcgacgcca cgcgccgcga cgccgaagag
    15661 gcacgcacgc tctacgagct gctgcaagac caggtggtgc cgctgtatta cgcgcgcaac
    15721 gacgccggtt tctcgcccgg ctgggtggcg ctggcgaagg aatcgatggc caccgtggcg
    15781 cctcgcttca actccatgcg catgctcgag gagtacgtcg gacagctcta cctcccggcc
    15841 gccgcgcgcg ggcagcggct cgccgccggc ggccatgccg cggcgcgcga gctcgccgag
    15901 tggcgcgcga aagtgcgcgc cgcgtggccg ggcgtcgtac tgcgccgcgc cggcgaaccc
    15961 ccgatgcgcg ccgcacatgg cgagggcgtg agaatcgcag tcgcggtgcg gctgaacggc
    16021 ctggcgcccg gggacctggc gctggagctg gtgctgcagc gcccagccgc gcagtcgccg
    16081 gcggcgcatt accgctttgc cccgcacggc ccgctcgccg agagcggcga gcacttgtac
    16141 gagctcgagt tcgcgcccga gcacaacggg cggctcgagt accggctgcg cgcctacccc
    16201 agccacgagc tgctcgcgca cccgttcgaa accgggctga tgatctggct gtagcggcgc
    16261 gtcgctcatc cgccgcttcg cacgcccggc tcggggttga tctagcgcaa tcggcgcgca
    16321 cgcgtgcggt gctgcaatga gccgccgggc gcggatattg ccgcgcccgc ctctgaggaa
    16381 agtcgtgtcg cgtgcgctga gcatgccggt cggacctgcg ccacgagtgc tgctcgtgac
    16441 ccccgaggtc tttccgctcg ccaagaccgg cggactggcc gacgtgtgcg ccgcgctgcc
    16501 gcgcgcgctc gcgcgccggg gcgccgacgt gcgcttcctg gtgcccgctt acacgcgggc
    16561 gctcgatcgc gtgcacgcgc cccgcgtggc cgctcggctc ggcccgctgc tcggcgtggc
    16621 gtcggcgcag ctgatccagg gcgcgatgcc cggttcaggg ctgccggtgt ggctgctcga
    16681 ttgtccggag ctctaccggc gcccgggcac gctctaccag gacccggacg gcaaagactg
    16741 gccggacaac tggcagcgtt tcgggttgct cgcgcacgcc gcggcgcgca tcgcgctcgg
    16801 cgaggcgcag ctcgactggc agcccgaggt ggtgcattgc cacgactggc acaccggact
    16861 cgcgccctat ctcatcggcc gcgctgccgc gccgcggccg cgcaccttgt tcacggtgca
    16921 caacgccgcg tttcagggca acttccccgc cgagcacgcg ccgcacctcg ggttgccccc
    16981 tgaggtgctc tcgcccgagg cgatggaatt ctggggtcag ctgtcgttcc tcaaggcggg
    17041 cgtgcgctat gcggaccgcg tcaacaccgt cagccccact tatgcgcgcg agctattcac
    17101 ggccgagttc ggctacggcc tggaagggct gttccggcac cgcggcagcg acttcaccgg
    17161 catcttgaac ggcatcgaca ccgagctctg ggatccggcc gcggacgacg gcatcgctgc
    17221 gcgcttctct gccgatgcgc cgcagggaaa ggccgactgc aagactgcgc tgcaggcagc
    17281 gctgcacctc gcacgggatc cgcaggcgcc gctcgtcgcc tttgccagcc gcatcacgtg
    17341 gcagaagatg gccgacgtga cgctggagcg gctgccggcg ctgctcgccg cgaactcgcg
    17401 cctgcagttc gcgctgcttg gcacgggcga gcgcgcactg gagcgcggct tcctggccct
    17461 ggcgccgcac taccccggcc gcgtctccgt gcgcatcgcc tacaccgagg acgaggagcg
    17521 tcggctgcag gcgggcgccg acgtgctgct gcacggctcg cgctacgaac cttgcggcct
    17581 cgcgcacaag atcgccatgc gctacggggc gctgccggtg gtgcgccgca ccggcggcct
    17641 ggccgatacg gtgcgcgagg gcgtcaacgg cttcatgttc gagtcgaccg cgggcgagga
    17701 cctgcaggcc gccgtggcac gctgcctcgc cacgtacgcg cagcggcccg acgcatggcg
    17761 cgcgatgcgc gaccacgcca tgtgcggcga ttacagctgg gagcagccag ccctcgagta
    17821 cctcgccctg taccgcgcgg cgccggttat tgcgtcagcc ggtagtacga gcggttgagg
    17881 tactcggcga cgtcctcgag gtcctcgagg gtgaacggcc ggccggtctg gccggcgcgc
    17941 agcacgacct ggtcgcgcag gtcggcgagc gagcgcacgc gcgacttcga gcgctcgcgc
    18001 tcgtgcaagc gctcgtagtg gcagccgccg cactgggtct tgtagaccag ctcgccgcga
    18061 tttggatcct gcgccgccgc cggcaggctg gcgaaggcaa gggcaaggca aaggcgcttc
    18121 atcggttgat ctccttcgga ccgcgctgca ggaagcaggt cagcgcgctg gacgaggcgg
    18181 cgaacgccca gcaaaggaag aagccgatgc tgtacacgcc gaggcggctc aacgtggtcg
    18241 gctctccgaa caggtacagc tcctgcgggt cgaacacggt gaagaagatt acctcgccga
    18301 tgccgccgac gatgaacgac ggccacag
//
LOCUS       DBNHKE010000071        12651 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_487908, whole genome shotgun sequence.
ACCESSION   DBNHKE010000071 DBNHKE010000000
VERSION     DBNHKE010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_487908"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="AC16O9_04655"
     CDS             complement(<1..1155)
                     /locus_tag="AC16O9_04655"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase CobB"
                     /protein_id="HIR6515941.1"
                     /translation="MNRLFISAAHKSSGKTTLCLGLAAALRAGGETVQPFKKGPDYID
                     PLWLGLAAGRPCYNLDPYLSGAEELRAEFALRMQGATLGLVEGNKGLYDGLALDGSNS
                     NAALAAMLGTPVVLVIDARGMTRGIAPLILGFQAFDPNIRIAGVILNQLGGARHEAKL
                     RAVIAHYTGVPVLGAVQYDEQLAIVERHLGLVPSNEAEGARARVEAIAARIAAQVDLE
                     ALLAVARSAGPLDAPAALKPQRTGEPSLRLGIARDSAFGFYYPGDLEALRAAGAQLVP
                     FDALRDKRLPAVDALFIGGGFPETHMDALAANAEMRGAVRDAIEAGMPAYAECGGLMY
                     LARSIQWHGRCAEMAGVLPADIVMHARPVGRGYVHLRETGRGPWPARAPAG"
     gene            complement(1152..2318)
                     /gene="nrfD"
                     /locus_tag="AC16O9_04660"
     CDS             complement(1152..2318)
                     /gene="nrfD"
                     /locus_tag="AC16O9_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6515942.1"
                     /translation="MASIQYREIEGTSRGFYGLLGVLALVIAGGLAAAWAMEHQGHVI
                     TGMTNRIVWGTPHVFAVFLIVAASGALNVASVASVFGRELYKPLARLSGLLAVTLLAG
                     GLAVLVLDLGRPDRLVVAMTHYNFKSIFAWNIFLYTGFFAVVALYLWTMFERRMNGYT
                     TRAGVLAFLWRLILTTGTGSIFGFLVAREPYDSALLAPLFIVMSFAFGTAAFVLVLMA
                     ACRWSARPLGDALLARLGNLLGIFVAAVLYFVAVHHLTNLYLARRLDVERFFLWDGGV
                     YTALFWLGQVLIGSVVPLVLVFAVRTRAALAAAAACVIAGGLAQIYVIIIGGQAYPQT
                     VFPGKEVSSSAFDGVVASYAPSLPEVALGMGGVGLALALTVIGLKVLPFLPTRA"
     gene            complement(2321..3046)
                     /gene="dsrO"
                     /locus_tag="AC16O9_04665"
     CDS             complement(2321..3046)
                     /gene="dsrO"
                     /locus_tag="AC16O9_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrO"
                     /protein_id="HIR6515943.1"
                     /translation="MNPGRRQVLAWGAAAAAGAAFAPGLRLVELALARSPEEAASALQ
                     RWGLLIDVAKCADCDECVSACHEENGVTGHGRAATDAQWIRKVSLRDRGTGHVQTLPL
                     MCQHCEHPPCVDVCPTGASFRRADGIVLVDKHTCIGCRYCVMACPFGARSFVHEPVTG
                     QKADVPRGKGTVESCTLCVHRVDRGETPACVEACNRDGKGAMTFGDLNDPASDIGARL
                     RREPSAALRADLALNPGVRYQHL"
     gene            complement(3043..3405)
                     /locus_tag="AC16O9_04670"
     CDS             complement(3043..3405)
                     /locus_tag="AC16O9_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515944.1"
                     /translation="MTRIAAALVAALLAVTGFADTGRVALPVIKIEQGEACVAPIEEM
                     RRNHTAMLLHQRDRTMREGIRSTRFSLKNCVECHASRKTGSVLGAEGFCSSCHAYTSV
                     KIDCFECHSSERQKAAAR"
     gene            complement(3416..5416)
                     /locus_tag="AC16O9_04675"
     CDS             complement(3416..5416)
                     /locus_tag="AC16O9_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6515945.1"
                     /translation="MSAPTAKPEAKSGHTFRKYKDGDSQWRSNQERIFAGDESDKCPV
                     YVHKTPPCQASCPSGEDIRGWLNIVRGVEKPPKGTAMQEYAFRRSTDANPFPSMMGRV
                     CPAPCQTGCNRNKVEDTVGINAVEQYIGDYALKQGYSFEAGADTGKRVAIVGGGPAGL
                     SAAYQLRRKGHAVTVFDDHAELGGMAKYGVPGYRLPRTHLDGEIGRIVAMGVEVRMGM
                     RVGRDIALKDLQAQFDAVLIAIGCKSGRPLAVPGGDAPNVISGVAFLEAFNQGRLKAV
                     AGRVVVVGGGDTSVDVVSVARRLGYVEGAADTERPENVVLGHTAHDVSKLAARQGADV
                     MLTSMQPIEEMNAAKHEIEDAQREGVKIQGNLLPKKVIVDERSGRAVALRLIEVEWAK
                     GKFTEKPGTERDIEAELIVVAIGQAGDFSGMEELDNGKGFVAADKYYQLASRPGLFAC
                     GDIIRPHLLTTVIGQGSVAADSIDRYLKGQEFAKRPKVDVHHFNLLDKLREAQLEPAK
                     YQAAPENGPRLIDQGVRGTALAGKAVGGFAIHNYEDRSAAEVCKSDELFLGHFPHQER
                     LKRTEVGPTAEQVLGHFEERVVGFTDKQAVDEAKRCMSCGMCFECDNCVIYCPQTAVK
                     RTPKSEATMGRYVYTDYDLCIGCHICADVCPTGYIKMGMGDH"
     gene            complement(5437..6930)
                     /gene="dsrK"
                     /locus_tag="AC16O9_04680"
     CDS             complement(5437..6930)
                     /gene="dsrK"
                     /locus_tag="AC16O9_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrK"
                     /protein_id="HIR6515946.1"
                     /translation="MAAAFETPKLGEYPVIPLVREGAMAHSKPFVAKAEHQAPLGFPG
                     ELVPDWEKVAVTRLGELVSESRALQVFLDSCVKCGACTDKCHYYLGTGDPKNMPVARQ
                     DLLRSVYRRYYTLAGKLFPKLVGARDMSKDVLDEWYSYFHQCSQCRRCSVFCPYGIDT
                     AEVSMAARDIMDSVGLGQKYSNEIIGKVHKIGNNLGLPEPALRDTLEGLEEDVKEETG
                     VDVRFPLDEEGAEVLVITPSADFFAEPHIDGLIGYAKVFHAAGISWTLSSRASEAANF
                     SLFIGNHDNLHKVAARIREAALELGVKRIVVGECGHAWRVAYSFWNTLIGPFDFLDAR
                     YPAPQHICEVTWDLIQRGAIRLDPSANDDKVVTFHDSCNVARGSRMGGAPGGQFTIPR
                     EVIRASVNKFVDMPPETIGEATFCCGGGGGLLTDDLVELRVKGALPRMQALQQVVDEH
                     GVNYLAAICAICKSQFTKVLPYYKHPMDMIGSVHGLVSRAIVLGARQ"
     gene            complement(6932..7657)
                     /locus_tag="AC16O9_04685"
     CDS             complement(6932..7657)
                     /locus_tag="AC16O9_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR6515947.1"
                     /translation="MSALTVSYALLYYAATLILVAGVLARCWRYARTPQPLKIPVPPA
                     PTTRGGVVLRMLAEVTLFRSLFRSNKWIWLFGWTFHVALALVLARHLRYFTEPVWAWV
                     ALIQPLGVYAGFAMLAGLVGLWARRFLVDRVRYISSPSDHLMLALLVAIAASGLAMKY
                     LAHTDIVALKAFFLGLMVFDWRPLPADPVLLLHLALVAALMIVFPVSKLLHAPGVFFS
                     PSLNQVDDARERRHVAGWSARLS"
     gene            complement(7674..8006)
                     /locus_tag="AC16O9_04690"
     CDS             complement(7674..8006)
                     /locus_tag="AC16O9_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR6515948.1"
                     /translation="MSIEVQGQTFETDEEGYLANRADWSEDVAKEMARLDNCDLTDAH
                     WEVINFLREYYEEYQIAPAVRVLTKAIGKKLGADKGNSKYLYELYPYGPAKQACKYAG
                     LPKPTGCV"
     gene            complement(8023..8331)
                     /gene="tusB"
                     /locus_tag="AC16O9_04695"
     CDS             complement(8023..8331)
                     /gene="tusB"
                     /locus_tag="AC16O9_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HIR6515949.1"
                     /translation="MRKMLHTVNKSPFEHSALETCLRYARPGSSVLLIEDGVYAAARD
                     TAVAKDLQDALQRVQVYALKPDVEARGVQNRVMDGVRLVDYGGFVDLVVEHDAVQSWL
                     "
     gene            complement(8334..8717)
                     /gene="tusC"
                     /locus_tag="AC16O9_04700"
     CDS             complement(8334..8717)
                     /gene="tusC"
                     /locus_tag="AC16O9_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HIR6515950.1"
                     /translation="MTEESGGGKVKKFLYVNRKAPYGTIYALESLEVVLIGAAFEQDV
                     SLAFVGDGVYQLVKGQDTKALEVKNFSPAFRALEDYDVTKLYVDEESLRARGLKADDL
                     VVPVELVSAERMAEIMEGQDVVLSF"
     gene            complement(8710..9102)
                     /gene="tusD"
                     /locus_tag="AC16O9_04705"
     CDS             complement(8710..9102)
                     /gene="tusD"
                     /locus_tag="AC16O9_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HIR6515951.1"
                     /translation="MKLGILVNEGPYTHEAADSAYQFARAALAKGHEVCRVFFYHDGV
                     NNGTRLTVPPQDDRNIVKRWSELAAEHKLDLVLCIAAAQRRGLMDKNEAKRHGKDTDN
                     LAPGFRISGLGQLIEAGIEADRMVVFGD"
     gene            complement(9114..10208)
                     /gene="dsrB"
                     /locus_tag="AC16O9_04710"
     CDS             complement(9114..10208)
                     /gene="dsrB"
                     /locus_tag="AC16O9_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HIR6515952.1"
                     /translation="MAQAQAVEKKQMRPPIESGCPDGMQYMHPLMVKNFGNWKYHDRP
                     RPGVLHHVAYSGDEIWTVRVGTQRQLGPYEINLLCDIVDKYGEGYVRFTIRSNLEVMV
                     STWDKVGPLIQAFNDAGYPVGGTGNSVTMISHTQGWLHCDIPGTDASGVVKSLMDELY
                     EEFIKEEMPNRVHITTSCCQINCGGQGDIAINVQHTKPPKINHDLVANVCERPSVVAR
                     CPVAAIRPAIVNGKPSLEVDEKKCICCGACYPPCPPMQINDPEHSKLAIWVGGKNSNA
                     RSKPMFQKLVAAGLPNNPPRWPEVAEVVKKILTTYKKDARHWERVGDWVERIGWPRFF
                     ELTGLPFTKFHIDNWRGARNSLNASTHIRF"
     gene            complement(10222..11526)
                     /gene="dsrA"
                     /locus_tag="AC16O9_04715"
     CDS             complement(10222..11526)
                     /gene="dsrA"
                     /locus_tag="AC16O9_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HIR6515953.1"
                     /translation="MSDARTPPKNPERHSTYVPKEKKSTKAFHPTPMLDQLESGPWPS
                     FVTGLKRLAHENNMMVDLLGQLEHSYKTRLGYWKGGTVSVFGYGGGIIPRFSEVASMF
                     PESKEFHTLRVQPPAGNHYTTKMLRQLSDSWKKWGSGLIAFHGQTGNIMFIGSTTENT
                     QHFFDEINEYGFDLGGAGPCVRTAMSCVGAARCEQSCANEHRIHRTLVNNFIDDMHRP
                     ALPYKFKFKVSGCPNDCMNSIQRADFAVIGTWRDDMQVDQKEVKKYVEGHGRKYTIDN
                     VIARCPTQALSLNDDDTLEVDNKSCVRCMHCINVMTKALKPGKDRGITLLIGGKRTLK
                     IGDTMGTVIVPFMKMDTDEDYEKLRELAATVIDFWAENGLEHERCGEMIERIGLATFL
                     EGIGVEVDPNMIAHPRTSSYVRLDDWDQEAQKWIDRKQKSAA"
     gene            11693..12583
                     /locus_tag="AC16O9_04720"
     CDS             11693..12583
                     /locus_tag="AC16O9_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR6515954.1"
                     /translation="MNAVLQNESPFALEPRQAYLRWRERKLERYPRSAEELVVQVRDP
                     RALRDSEVAELRRVCAAANMAVYASALADAADTTLVQRVAERLGLRRLQANPLADEDG
                     ISALAVDPEKSGRGYIPYSSRRLLWHTDGYYNPPAQRIRAFLLHCVRPAAAGGENRLL
                     DPEIAYLLLRDEDPCHVEALSAPDAMTVPANEEDPSRGRAAQTGPVFSDEAGTLHMRY
                     TARTRSIGWRADAPTQAAAQRLRALLDSESPYIFRLRLSAGQGLVCNNVLHDRSEFTD
                     TPGAGRLVYRARYADRIAAA"
BASE COUNT         1875 a         4369 c         4351 g         2056 t
ORIGIN      
        1 cccggccggg gcgcgcgcgg gccacggccc gcgcccggtc tcgcgcaggt gcacgtagcc
       61 gcgcccgacg gggcgtgcat gcatgacgat gtcggcgggc agcacgcccg ccatctcggc
      121 gcaccggccg tgccactgga tcgagcgcgc gaggtacatc agtccgccgc actcggcgta
      181 cgcgggcatc cccgcctcga tcgcgtcgcg caccgcgccg cgcatctccg cgttcgccgc
      241 gagcgcatcc atgtgcgtct cagggaagcc gccgccgatg aacagcgcgt ccaccgccgg
      301 caggcgcttg tcgcgcagcg cgtcgaacgg cacgagctgc gcgcccgccg cacgcagcgc
      361 ctcgaggtcg ccgggatagt agaagccgaa cgccgagtcg cgcgcgatgc cgaggcgcag
      421 cgacggctcg cccgttcgct gcggcttcag cgcagcgggc gcgtcgagcg gccctgccga
      481 gcgcgcgacg gcaagaagcg cttcgagatc cacctgcgcg gcgatgcgcg ccgcgatcgc
      541 ctcgacgcgc gcgcgcgcgc cctcggcttc gttgctcggc acgagcccga ggtggcgctc
      601 cacgatcgcg agctgctcgt cgtactgcac cgcgccgagc accggcacgc cggtgtagtg
      661 cgcgatcacc gcccggagct tggcctcgtg gcgcgcgccg ccgagctggt tgaggatcac
      721 gccggcgatg cggatgttgg ggtcgaaggc ctggaagccg aggatgagcg gcgcgatgcc
      781 gcgcgtcatg ccgcgcgcgt cgatcaccag caccacgggc gtgccgagca tcgccgcgag
      841 ggcggcgttg ctgttgctgc cgtccagcgc gagcccgtcg tacaggccct tgttgccttc
      901 caccaggccg agcgtggcgc cctgcatgcg cagcgcgaac tcggcgcgca gttcctccgc
      961 cccgctcagg tagggatcga ggttgtagca cggccgaccg gcagcgagcc cgagccacag
     1021 cggatcgatg tagtcggggc ccttcttgaa gggctgtacc gtctcgccgc ccgcgcgcag
     1081 cgccgcggcg aggccgaggc acagcgtggt cttgcccgag gacttgtgcg cggcggagat
     1141 gaagagccgg ttcatgcccg ggtcggaagg aacggcagca ccttgagccc gatgacggtg
     1201 agcgcgagcg ccagccccac gccgcccatg ccgagcgcca cctcgggcag gctcggcgca
     1261 tagctcgcca ccacgccgtc gaaggcgctg ctcgagacct ccttgcccgg gaagacggtc
     1321 tgcggatagg cctggccgcc gatgatgatg acgtagatct gcgcaagacc gcccgcgatg
     1381 acgcaggcgg cggccgcggc gagcgcggcc cgcgtgcgca ccgcgaagac gaggacgagc
     1441 ggcacgacgc tgccgatgag cacctggccg agccagaaca gcgccgtata cacgccgccg
     1501 tcccagagga agaagcgctc cacatccagc cggcgcgcga ggtacaggtt ggtgaggtgg
     1561 tgcaccgcga cgaaatagag caccgcggcg acgaagatgc cgagcagatt gcccaggcgc
     1621 gcgagcagcg cgtcgccgag cggccgggcg ctccagcggc acgccgccat cagcaccagc
     1681 acgaacgccg ccgtgccgaa agcgaacgac atgacgatga acagcggcgc gagcagcgcg
     1741 ctgtcgtagg gctcgcgcgc gaccagaaag ccgaagatcg agcccgtgcc cgtggtgagg
     1801 atcaggcgcc acaggaacgc gagcacgccc gcgcgcgtgg tgtagccgtt catgcgccgc
     1861 tcgaacatcg tccacaggta cagcgccacc acggcaaaga agccggtgta caggaagatg
     1921 ttccaggcga agatggactt gaagttgtag tgcgtcatcg ccacgacgag ccggtcgggg
     1981 cggccgaggt cgaggaccag caccgcgagc ccgccggcga gcagcgtcac cgcgagcagc
     2041 cccgagaggc gtgcgagcgg cttgtagagc tcgcgcccga acaccgaggc taccgaggcg
     2101 acgttcagcg cgcccgaggc cgcgacgatc aggaacacgg cgaagacgtg cggcgtgccc
     2161 cagacgatgc ggttggtcat gccggtgatg acatggccct ggtgctccat cgcccaggcg
     2221 gccgcgagcc cgcccgcgat cacgagcgca agcacgccga gcaggccata gaagccccgg
     2281 ctggttccct cgatctcgcg atactggatg ctcgccatgg ctacaggtgc tggtagcgca
     2341 cgccggggtt gagcgcgaga tccgcgcgca gcgcggccga gggctcgcgg cgcaggcgcg
     2401 cgccgatgtc gcttgccggg tcgttcaggt cgccgaacgt catcgcgccc ttgccgtcgc
     2461 ggttgcaggc ctcgacgcac gccggggtct cgccgcgatc cacgcgatgc acgcacagcg
     2521 tgcaggattc gacggtgccc ttgccgcgcg gcacatcggc cttctggccg gtcaccggct
     2581 cgtggacgaa cgagcgcgcg ccgaacgggc aggccatgac gcagtagcgg cagccgatgc
     2641 acgtgtgctt gtcgacgagc acgatgccgt cggcgcgccg gaacgacgcg cccgtggggc
     2701 acacgtccac gcacggcggg tgctcgcagt gctggcacat gagcggcagc gtctgcacgt
     2761 ggccggtgcc gcggtcgcgc aggctcacct tgcggatcca ctgcgcgtcg gtcgccgcgc
     2821 ggccgtggcc cgtcacgccg ttctcctcgt ggcaggcgct gacgcactcg tcgcagtcgg
     2881 cgcacttcgc gacgtcgatc agcagccccc agcgctgcag cgcggaggcc gcctcctcgg
     2941 gcgagcgcgc gagcgcgagc tcgaccagcc gcaggccggg cgcgaacgca gcacccgcgg
     3001 cggcggccgc gccccaggcg aggacctgcc ggcggccggg attcatcgcg ccgccgcctt
     3061 ctgccgctcc gagctgtggc actcgaagca gtcgatcttc acggaggtgt aggcgtggca
     3121 gctcgagcaa aagccctccg cgccgagcac gctgccggtc ttccggctgg cgtggcactc
     3181 cacgcagttc ttcaggctga agcgcgtgct gcggatgccc tcgcgcatcg tgcggtcgcg
     3241 ctggtgcagg agcatcgccg tgtggttgcg ccgcatctct tcgatgggcg cgacgcaggc
     3301 ctcgccctgc tcgatcttga tgacgggcag cgcgacccgg ccggtgtcgg cgaagcccgt
     3361 gactgcgagc aacgcggcga cgagggcggc ggcaatgcgc gtcatgcccg ggcgctcagt
     3421 ggtcgcccat ccccatcttg atgtagccgg tggggcagac gtcggcgcag atgtggcagc
     3481 cgatgcacag gtcgtagtcg gtgtagacgt agcggcccat cgtcgcctcc gacttcggcg
     3541 tgcgcttgac cgcggtctgc gggcagtaga tgacgcagtt gtcgcactcg aagcacatgc
     3601 cgcagctcat gcagcgcttg gcctcgtcca ccgcctgctt gtcggtgaac ccgaccacgc
     3661 gctcctcgaa gtgcccgagc acttgctcgg ccgtggggcc gacctcggtg cgcttgaggc
     3721 gctcctggtg cgggaagtgg ccgaggaaca gctcgtcgga cttgcacact tcggcggcgc
     3781 tgcggtcctc gtagttgtgg atggcaaagc cgcccaccgc cttgccggcg agcgcggtgc
     3841 cgcgcacgcc ctggtcgatg aggcgcgggc cgttctccgg tgcggcctgg tacttcgccg
     3901 gctcgagctg cgcctcgcgc agcttgtcga gcaggttgaa gtggtgcacg tccaccttcg
     3961 gacgcttggc gaactcctgg cccttcaggt agcggtcgat gctgtcggcg gcgacggagc
     4021 cctggccgat caccgtggtg agcaggtgcg ggcggatgat gtcgccgcag gcgaacagcc
     4081 ccgggcggct ggccagctgg tagtacttgt cggccgccac gaagcccttg ccgttgtcga
     4141 gctcctccat gccggagaaa tcgcccgcct gcccgatcgc gacgacgatg agctcggcct
     4201 cgatgtcgcg ctcggtgccc ggcttctccg tgaacttgcc cttcgcccac tcgacttcga
     4261 tcaggcgcag cgccactgcg cggccgctgc gctcgtcgac gatcactttc ttgggcagca
     4321 gattgccctg gatcttgacg ccctcgcgct gcgcatcctc gatctcgtgc tttgccgcat
     4381 tcatctcctc gatgggctgc atcgaggtca gcatcacgtc ggcgccctgg cgcgcggcga
     4441 gcttggagac gtcgtgcgcg gtgtggccca gcacgacgtt ctcgggccgc tcggtgtccg
     4501 ccgccccctc cacgtagccc aggcgccgcg ccaccgacac cacgtccacc gaggtatcgc
     4561 cgccgccgac caccaccacg cgcccggcca cggccttgag ccgcccctgg ttgaacgcct
     4621 cgagaaaggc gacgccgctg atgacgttgg gcgcatcgcc ccccggcacc gccagcggcc
     4681 gcccggactt gcagccgatt gcgatcagca ccgcgtcgaa ctgcgcttgc agatccttga
     4741 gcgcgatgtc acggcccacg cgcatgccca tgcgcacctc cacgcccatg gcgacgatgc
     4801 gtccgatctc gccgtcgagg tgcgtgcgcg gcagccggta cccgggcacg ccgtacttcg
     4861 ccatgccgcc gagctccgcg tgatcgtcga agacggtcac tgcgtggccc ttgcggcgca
     4921 gctggtaggc ggccgacagg cccgccgggc cgccgccgac gatggccacg cgcttgccgg
     4981 tgtccgcgcc cgcctcgaac gaatagccct gcttgagcgc gtagtcgccg atgtactgct
     5041 cgaccgcgtt gatgccgacg gtgtcctcga ccttgttgcg gttgcagccg gtctggcagg
     5101 gcgccgggca cacgcgcccc atcatcgagg gaaagggatt ggcgtcggtg gagcgccgga
     5161 acgcgtactc ctgcatcgcc gtgcccttgg gcggcttttc caccccgcgc acgatgttga
     5221 gccagccgcg gatgtcctcg cccgaggggc agctcgcctg gcacggcggc gtcttgtgca
     5281 cgtacaccgg gcacttgtcg gactcgtcgc cggcgaagat gcgctcctgg ttggagcgcc
     5341 actggctgtc gccgtccttg tacttgcgga acgtgtgtcc gctcttggcc tcgggcttcg
     5401 ctgtgggtgc tgacatggag actccgtgtg gatgcgctat tgcctggcgc cgagcacgat
     5461 cgccctgctg accaggccgt gcacgctgcc gatcatgtcc atggggtgct tgtagtaggg
     5521 caggaccttg gtgaactggc tcttgcagat ggcgcagatg gcggcgaggt agttgacgcc
     5581 gtgctcgtcg accacctgct gcagcgcctg catgcgcggc agcgcgccct tcacgcgcag
     5641 ctcgaccagg tcgtcggtga gcagcccccc gccgccgccg caacagaagg tggcctcgcc
     5701 gatcgtctcg gggggcatgt cgacgaactt gttgacgctg gcgcggatga cctcgcgcgg
     5761 gatggtgaac tgcccgcccg gcgccccgcc catgcgcgag ccgcgcgcca cgttgcacga
     5821 gtcgtggaag gtcaccacct tgtcgtcgtt cgcgctcggg tcgagccgga tcgcgccgcg
     5881 ctggatgaga tcccaggtca cctcgcagat gtgctgcggc gccgggtagc gggcatcgag
     5941 gaagtcgaac gggccgatga gcgtgttcca gaagctgtag gcgacgcgcc aggcgtggcc
     6001 gcactcgccc accacgatgc gcttcacgcc gagctcgagc gccgcctcgc ggatgcgcgc
     6061 ggcgaccttg tgcaggttgt cgtggttgcc gatgaagagc gagaagttcg cggcttcgga
     6121 ggcgcgcgac gagagcgtcc aggaaatgcc cgccgcatgg aacaccttgg cgtagccgat
     6181 caggccgtcg atgtgcggct cggcgaagaa gtcggcgctg ggcgtgatga cgagcacctc
     6241 ggcgccctcc tcgtcgagcg ggaagcgcac gtcgaccccg gtttcctcct tcacgtcctc
     6301 ttccaggccc tcgagcgtgt cgcgcagcgc cggctcgggc aaccccaggt tgttgccgat
     6361 cttgtgcacc ttgccgatga tctcgttgga gtacttctgt cccaggccca cgctgtccat
     6421 gatgtcgcgc gccgccatcg agacctcggc ggtgtcgatg ccgtaggggc agaacaccga
     6481 gcagcgccgg cactgcgagc actggtggaa gtagctgtac cactcgtcga gcacgtcctt
     6541 ggacatgtcg cgcgcgccca ccagcttcgg gaagagcttg ccggcgagcg tgtagtagcg
     6601 ccggtacacc gagcgcagca ggtcctggcg cgcgaccggc atgttcttcg ggtccccggt
     6661 gccgaggtag tagtggcact tgtcggtgca cgcgccgcac ttcacgcagg aatcgaggaa
     6721 cacctgcagc gcgcgcgact cggagaccag ctcgcccagg cgcgtcaccg cgaccttctc
     6781 ccagtcgggc accagctcgc ccgggaagcc gagcggcgcc tggtgctcgg ccttggcgac
     6841 gaagggcttc gaatgcgcca tcgccccttc gcgcaccagg ggaatgacgg ggtactcgcc
     6901 cagcttcggc gtctcgaagg cggcagccat gtcagctcag ccgcgcggac cagcccgcga
     6961 cgtgtcgccg ctcgcgcgcg tcgtccacct ggttgaggct cgggctgaag aacacgcccg
     7021 gcgcgtgcag gagcttggac accgggaaca cgatcatcag cgcggccacc agcgcgaggt
     7081 gcaggagcag caccggatcg gcgggcagcg gccgccagtc gaagaccatc agcccgagaa
     7141 agaacgcttt cagcgccacg atgtcggtgt gcgccaggta cttcatggcg agcccgctcg
     7201 cggcgatggc gacgaggagc gcgagcatca gatggtcgga ggggctgctg atgtagcgca
     7261 cgcggtcaac gaggaagcgc cgcgcccaca ggccgacgag cccggcgagc atggcaaagc
     7321 ccgcgtacac gcccaagggc tggatgagcg cgacccaggc ccacaccggc tctgtgaagt
     7381 agcgcaggtg ccgcgccagc accagcgcga gcgccacgtg gaaggtccag ccgaagagcc
     7441 agatccactt gttggagcgg aacaggctcc ggaacagcgt cacctccgcc agcatgcgca
     7501 gcacgacgcc gccgcgcgtg gtgggcgccg gcgggaccgg gatcttcagc ggctgcggcg
     7561 tgcgcgcata gcgccagcag cgcgcgagca cgccggcgac gaggatcagc gtcgccgcgt
     7621 agtagagcag cgcgtagctg acggtcagcg cggacaccgg tccgccccgc ggctcagacg
     7681 cagccggtcg gcttgggcag gcccgcgtac ttgcacgcct gcttggcggg gccgtaggga
     7741 tacagctcgt acaggtactt gctgttgccc ttgtccgcgc cgagcttctt gccgatcgcc
     7801 ttggtgagca cgcgcaccgc gggcgcgatc tggtactctt cgtagtactc gcgcaggaag
     7861 ttgatcactt cccagtgcgc gtcggtcaga tcgcagttgt ccagccgcgc catttccttg
     7921 gccacgtcct cggaccagtc ggcgcggttc gcgaggtagc cttcctcgtc ggtttcgaac
     7981 gtttggcctt gcacttcgat gctcatgtgt tcctccaggg gttcatagcc aagactgcac
     8041 ggcgtcgtgc tccaccacga ggtccacgaa ccccccgtag tccaccaggc gcacgccgtc
     8101 catcacccgg ttttgcacgc cccgcgcctc gacgtcgggc ttgagcgcgt agacctgcac
     8161 gcgctgcagg gcgtcctgca ggtccttcgc cacggcggtg tcgcgcgccg ccgcgtatac
     8221 gccgtcctcg atcagcagca cgctgctccc cggccgcgcg tagcgcaggc aggtctcgag
     8281 cgcgctgtgc tcgaagggcg acttgttcac cgtgtgcagc atcttgcgca ttctcagaag
     8341 ctcaggacca cgtcctggcc ctccatgatc tcggccatgc gctccgcgct caccagctcg
     8401 accgggacca ccaggtcgtc ggccttgagc ccgcgcgcgc gcagcgactc ctcgtcgacg
     8461 tagagcttgg tcacgtcgta gtcctcgagc gcgcggaacg cgggcgaaaa gttcttcacc
     8521 tccagcgcct tggtgtcctg gcccttgacg agctggtaga cgccgtcgcc gacgaaggcg
     8581 aggctcacgt cctgctcgaa ggccgcgccg atcagcacca cctcgagcga ctcgagcgcg
     8641 tagatcgtgc cgtacggcgc cttgcggttc acgtacagga acttcttgac cttgccgccg
     8701 ccgctctctt cagtcaccga acaccaccat ccggtcggcc tcgatgccgg cctcgatcag
     8761 ctgcccgagg cccgagatgc ggaagcccgg cgcgaggttg tcggtgtcct tgccgtggcg
     8821 cttggcctcg ttcttgtcca tgaggccgcg gcgctgcgcc gcggcgatgc acagcaccag
     8881 atcgagcttg tgctccgcgg cgagctcgct ccagcgcttg acgatgttgc ggtcgtcctg
     8941 cggcggcacc gtgagccgcg tgccgttgtt cacgccgtcg tggtagaaga acacgcggca
     9001 gacctcgtgg cccttggcga gcgcggccct cgcgaactgg taggcgctgt ccgcggcctc
     9061 gtgcgtgtac ggcccttcgt tcaccaggat gccgagcttc attcccgcgc gtcctagaag
     9121 cggatgtgag tcgaggcgtt gaggctgttc ctcgccccgc gccagttgtc gatgtggaac
     9181 ttggtgaacg gcagcccggt gagctcgaaa aagcgcggcc agccgatgcg ctccacccag
     9241 tcgccgacgc gctcccagtg gcgcgcgtcc ttcttgtagg tggtgaggat cttcttcacc
     9301 acctcggcca cctccggcca gcgcggcgga ttgttgggca ggcccgcggc caccagcttc
     9361 tggaacatcg gcttggagcg cgcgttggag ttcttgccgc ccacccagat ggcgagcttg
     9421 gagtgctccg ggtcgttgat ctgcatcggc gggcacggcg ggtagcacgc gccgcagcag
     9481 atgcacttct tctcgtccac ctcgagcgag ggcttgccgt tcacgatcgc cgggcggatg
     9541 gccgccaccg ggcagcgcgc gaccaccgaa ggccgctcgc agacgttcgc cacgaggtcg
     9601 tggttgatct tggggggctt ggtgtgctgc acgttgatgg cgatgtcgcc ctggccgccg
     9661 cagttgatct ggcagcagga ggtggtgatg tgcacgcggt tgggcatttc ctccttgatg
     9721 aactcctcgt acagctcgtc catcagggac ttcaccacgc cggaggcgtc ggtgccgggg
     9781 atgtcgcagt gcagccagcc ctgcgtgtgc gagatcatgg tcaccgagtt gccggtgccg
     9841 ccgaccgggt agccggcgtc gttgaacgcc tggatgaggg gccccacctt gtcccacgtc
     9901 gacaccatca cctcgaggtt cgagcggatg gtgaagcgca cgtagccctc gccgtacttg
     9961 tcgacgatgt cgcacagcag gttgatctcg tacggcccga gctggcgctg cgtgccgacg
    10021 cgcaccgtcc agatctcgtc gcccgagtac gccacgtggt gcagcacgcc ggggcggggc
    10081 cggtcgtggt acttccagtt gccgaagttc ttcaccatca gcggatgcat gtactgcatg
    10141 ccgtccgggc acccgctctc gatcggcggg cgcatctgct tcttctctac ggcctgtgct
    10201 tgtgccatgg cgctctcctc gtcaggccgc ggacttctgc ttgcgatcga tccacttctg
    10261 cgcttcctgg tcccagtcgt ccaggcgcac gtaggagctg gtgcgcgggt gggcgatcat
    10321 gttgggatcg acctcgacgc cgatgccttc gaggaaggtg gcgaggccga tgcgctcgat
    10381 catctcgccg cagcgctcgt gctccaggcc gttctcggcc cagaagtcga tcaccgtggc
    10441 ggcgagctcg cgcagcttct cgtagtcctc gtcggtgtcc atcttcatga acggcacgat
    10501 caccgtgccc atggtgtcgc cgatcttgag cgtgcgcttg ccgccgatca gcagcgtgat
    10561 gccgcggtcc ttgccgggct tgagcgcctt ggtcatgacg ttgatgcagt gcatgcagcg
    10621 cacgcagctc ttgttgtcga cctccagcgt gtcgtcgtcg ttcagcgaga gcgcctgggt
    10681 cgggcagcgc gcgatcacgt tgtcgatggt gtacttgcgg ccgtggccct cgacgtactt
    10741 cttcacctct ttctggtcga cctgcatgtc gtcgcgccag gtgccgatga cggcgaagtc
    10801 ggcgcgctgg atcgagttca tgcagtcgtt cgggcagccc gagaccttga acttgaactt
    10861 gtagggcagc gccgggcggt gcatgtcgtc gatgaagttg ttgaccagcg tcctgtggat
    10921 gcggtgctcg ttggcgcagg actgctcgca gcgcgccgcg cccacgcagg acatcgccgt
    10981 gcgcacgcac gggcccgcgc ccccaaggtc gaagccgtac tcgttgatct cgtcgaagaa
    11041 gtgctgcgtg ttctcggtgg tcgagccgat gaacatgatg ttgccggtct ggccgtggaa
    11101 ggcgatgagc cccgagcccc acttcttcca ggaatccgag agctgccgga gcatcttggt
    11161 cgtgtagtgg ttgcccgccg gcggctgcac gcgcagcgtg tggaactcct tggattccgg
    11221 gaacatgctc gcgacttccg aaaagcgcgg gatgatgccc ccgccatagc cgaacaccga
    11281 gaccgtgccg cccttccagt accccaggcg cgtcttgtag gagtgctcga gctggccgag
    11341 caggtccacc atcatgttgt tctcgtgcgc gaggcgcttc aggcccgtga cgaagctcgg
    11401 ccacgggccg ctctcgagct ggtcgagcat gggcgtcgga tggaaggcct tggtggactt
    11461 cttctccttg ggcacgtagg tgctgtgccg ctcgggattc ttcggcggcg tgcgggcgtc
    11521 ggacatcgcg ttccctcctc caggtgcggg gtcgagcttt cgaagtgtaa tgaccctgat
    11581 ccccgcgagg gactgatttt ggggatagtt gggggcccgc gcctcccccc tttggagata
    11641 tagggcgggt ttatgaccgg cgcccggcaa atgcggtagc gtttgtgacc ccgtgaacgc
    11701 cgttttgcag aacgaatcgc ccttcgcgct cgagccgcgg caggcctacc tgcgctggcg
    11761 cgagcgcaag ctcgagcgct acccgcgcag cgccgaagag ctcgtcgtgc aggtgcgcga
    11821 cccgcgcgcg ctgcgcgaca gcgaagtcgc ggaattgcgc cgcgtgtgtg cggcggcgaa
    11881 catggcggtc tacgcaagcg cgctcgccga cgcggccgac acgaccctcg tgcagcgcgt
    11941 agccgagcgc ctgggcctgc gccgtctgca ggcgaatccc ctggccgacg aggacggcat
    12001 cagcgcactg gcggtggacc cggagaagtc cggccgcggc tatatcccgt actcgagccg
    12061 ccggctgctg tggcacaccg acggctacta caacccgcct gcacagcgca tccgcgcctt
    12121 cctcctgcat tgcgtgcgcc cggccgccgc gggcggcgaa aaccgactcc tcgatccgga
    12181 gatcgcgtat ctcctgctgc gcgacgagga tccgtgtcat gtcgaggcgc tctcggcccc
    12241 cgatgcgatg accgtgccgg cgaacgagga ggatccgtcg agggggcgtg cggcgcagac
    12301 cggcccggtg ttctcggacg aagccggcac gctgcacatg cgctacacgg cgcgcacgcg
    12361 cagcatcggg tggcgcgcgg acgcccccac gcaggccgcc gcgcagcgct tgcgggcgct
    12421 gctcgactcg gagtccccct acatcttccg gctgcgcctc tcggcgggcc agggcctcgt
    12481 gtgcaacaac gtgctgcacg accgcagcga gttcacggac acgcccggcg cgggccggct
    12541 cgtataccgg gcgcgatacg ccgatcgcat cgccgccgca tgaaatacat cccgctcaag
    12601 tacatcaagg aaagcgccgc cgtcggcaac gccgacgaca gcaccgacgc g
//
LOCUS       DBNHKE010000072         3071 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_857130, whole genome shotgun sequence.
ACCESSION   DBNHKE010000072 DBNHKE010000000
VERSION     DBNHKE010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_857130"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(506..595)
                     /locus_tag="AC16O9_04725"
     tRNA            complement(506..595)
                     /locus_tag="AC16O9_04725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Ser,seq:cga)
     gene            complement(614..1891)
                     /gene="serS"
                     /locus_tag="AC16O9_04730"
     CDS             complement(614..1891)
                     /gene="serS"
                     /locus_tag="AC16O9_04730"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR6515955.1"
                     /translation="MIDIQLLRNDIDAVAGRLATRGFALDIAAFKALEDARKAIQTRT
                     EQLQAQRNALSKQVGQAKAKKDEAQAAALMSQATQVGEKLKALEAENEKTQAALREFV
                     SLLPNLTHESVPVGSSSEQNVEVRRWGAPRQFDFKPKDHVDVGTPLGLDFETGARLAG
                     ARFAVMKGQLARLHRALVQFMLDVHTTEHGYTEVYTPYMVSGECADGVSSLAKFKGDL
                     FKLEERDLYLIPTAEYPVTNFVREQILPLEALPMKFVCHSPCFRSEAGAAGKDTRGMI
                     RNHQFDKVELVQIVHPETSYAGHEALTAHAEVILQRLELPYRVITLCTGDTGFSSAKT
                     YDLEVWLPGQDAYREISSCSNFEAFQARRMQARFRNEKGRPEPVHTLNGSGLAVGRTL
                     VAILENFQNAHGSVTIPPALRPYMGGVEKLARA"
     gene            complement(1888..>3071)
                     /locus_tag="AC16O9_04735"
     CDS             complement(1888..>3071)
                     /locus_tag="AC16O9_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR6515956.1"
                     /translation="NLLIESIQKDTLFSMILWGPPGTGKTTLARLMAQAFDAEFIALS
                     AVFSGVKDIRDAVARAEDVLAVRGRPTILFVDEVHRFNKAQQDAFLPYVEKGLFTFIG
                     ATTENPSFEVNSALLSRATVYVLESLAAEDLATLLERTGLEAEEGARARLIGFADGDA
                     RRLLNAVEILQNAKPRGTVDAAFVEATLAKNLRRFDKGGEQFYDQISALHKAVRGSDP
                     DASLYWLVRMLDGGADPRYLARRIVRMAVEDVGLADPRALHLALDAAETYERLGSPEG
                     ELALAEAVLYLSVAAKSNAVYNAYNAARAFVQEDGTRPVPLHIRNAPTKLMKNLGYGK
                     DYRYAHDEADAFAAGENYFPEGMPAVEWYKPTQRGLEQRIGQKLEELRRLNAAARAKS
                     T"
BASE COUNT          474 a         1032 c         1011 g          554 t
ORIGIN      
        1 gcccccttat gcgtgtcttt ccagagtttg cacggcgtgc cgcgcctggt cttcgcgccg
       61 caggtccggc gcaggatgta caacgcgaac gccttgtccg tgaagatggc cgttaagacg
      121 gagtggaacc actttccggg cgtgctggca ccggtgacgg agtagaacga cttcccaaag
      181 gtgctaccac cggccatcaa ttgacctcct cgcgcgccat ggggagcgcg ttgccctgaa
      241 attttgtcgc gcgccgcccg aaaacgcgaa tctggtagat cgccccgccc tagctgcatc
      301 cgggcacagg attgaggacc cctcctcata gggggggcgg tcgaagccga aaaaggatgc
      361 taccccccgg gggtctactt ctacctaggc gggataccga ccgcgcgcca gttctgcaag
      421 gccacccgaa tatgggtagc tgtatgggta agacgtagcg tcggggaccg aaatctagac
      481 gtttcagaac gttaggggac ttgactggcg gagagggagg gattcgaacc cccggtacgc
      541 ttacgcgcac aacagatttc gagtctgccg caatcgacca ctctgccacc tctccgcggc
      601 gcaggcgatt ttatcaggcg cgcgcgagct tctcgacccc gcccatgtac gggcgcagcg
      661 ccggcggaat agtcaccgac ccgtgcgcgt tctggaagtt ctcgaggatc gcgacgagcg
      721 tgcggcccac ggcgagcccg gagccgttca gcgtgtgcac gggctcgggc ctgccctttt
      781 cattcctgaa gcgcgcctgc atgcggcgcg cctggaacgc ttcgaagttg gagcacgagc
      841 tgatctcgcg gtaggcgtcc tgacccggca gccagacctc caggtcgtag gtcttggcgg
      901 acgaaaatcc agtgtcgccg gtgcatagcg tgatcacccg gtacggcagc tcgagccgtt
      961 gcaggatcac ttcggcgtgc gcggtgagcg cttcgtgccc cgcgtacgat gtctccgggt
     1021 gcacgatctg caccagctcc accttgtcga actggtggtt gcggatcatg ccgcgcgtgt
     1081 ccttgcccgc ggcgcccgcc tccgagcgga agcacggcga gtggcacacg aacttcatcg
     1141 gcagcgcctc aagcggcagg atctgctcgc gcacgaagtt ggtcaccggg tattccgcgg
     1201 tcgggatcag gtagaggtcg cgctcttcga gcttgaagag atcgcccttg aacttggcga
     1261 ggctcgagac gccgtcggcg cactcgccgc tcaccatgta gggcgtgtac acctcggtgt
     1321 agccgtgttc cgtcgtatgc acgtcgagca tgaactggac gagcgcgcgg tgcaggcgcg
     1381 cgagctgccc cttcatcacc gcgaagcggg cgccggcgag ccgcgctccg gtttcgaaat
     1441 ccaggccgag cggcgtgccg acgtcgacgt gatccttcgg cttgaagtcg aactggcgcg
     1501 gcgcgcccca gcggcgcacc tccacgttct gctccgagga gctcccgacc ggcacgctct
     1561 cgtgcgtcag gttcggaagc agcgacacga actcgcgcag cgccgcctgc gttttctcgt
     1621 tctccgcttc cagtgccttc agcttctcgc cgacctgcgt ggcctgcgac atcagggccg
     1681 cggcctgcgc ttcgtccttc ttcgccttcg cctgcccgac ctgcttcgac agggcgttgc
     1741 gctgcgcctg caactgctcg gtcctggtct ggattgcctt gcgcgcgtct tccagcgcct
     1801 tgaaggcggc tatgtcgagc gcgaagccgc gcgtcgcgag gcgcccggcg acggcgtcga
     1861 tatcgttccg cagcagctgg atgtcgatca tgtgctcttc gcgcgcgctg cggcgttcag
     1921 ccgccgcagt tcttcgagct tctgcccgat cctttgctcg agcccgcgct gcgtgggctt
     1981 gtaccactcg accgccggca tgccctccgg aaagtagttc tcgcccgcgg cgaaggcgtc
     2041 ggcttcgtcg tgcgcgtagc ggtagtcctt gccgtaaccc aggttcttca tcagcttggt
     2101 gggcgcgttg cggatgtgca gcggcaccgg ccgcgtgccg tcttcctgca cgaacgcgcg
     2161 cgccgcatta taggcgttgt acacggcgtt cgattttgcg gcgacggaaa ggtagagcac
     2221 ggcctcggcg agcgcgagct cgccctcggg cgatcccagg cgctcgtagg tctcggcggc
     2281 atcgagcgca aggtgcagcg cgcgaggatc ggcgaggccc acgtcctcca ccgccatgcg
     2341 cacgatgcgc cgcgcgaggt agcgcggatc ggcgccgccg tcgagcatgc gcaccagcca
     2401 gtacagcgac gcatcggggt ccgagccgcg cactgccttg tgcagggccg agatctggtc
     2461 gtagaactgc tcgccgccct tgtcgaagcg acgcaggttc ttcgccagcg tcgcctcgac
     2521 gaacgcggcg tcgacggtgc cgcgcggttt cgcgttctgc agtatctcca ccgcgttgag
     2581 caagcgccgt gcatcgccgt cggcgaagcc gatcaggcgc gcgcgcgcgc cctcttccgc
     2641 ctcgagcccg gtgcgctcga gcagcgtcgc gagatcctct gccgccaggc tctccagcac
     2701 gtacacggtg gcccgcgaca ggagcgcgga gttcacctcg aagctcggat tctcggtggt
     2761 cgcgccgatg aaggtgaaca ggcccttctc cacgtagggc aggaaggcgt cctgctgcgc
     2821 cttgttgaag cggtgcacct cgtccacgaa caggatcgtc ggccggccgc gcacggcaag
     2881 cacgtcctcg gcgcgcgcca ccgcgtcacg gatgtccttg acccccgaga acaccgcgga
     2941 gagcgcgatg aactcggcgt cgaaggcctg cgccatcagg cgcgcgagcg tcgtcttgcc
     3001 cgtgccgggc gggccccaca ggatcatcga gaacagggtg tccttctgga tcgactcgat
     3061 gagcaggttg c
//
LOCUS       DBNHKE010000073         5335 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_488167, whole genome shotgun sequence.
ACCESSION   DBNHKE010000073 DBNHKE010000000
VERSION     DBNHKE010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_488167"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="AC16O9_04740"
     CDS             complement(<1..916)
                     /locus_tag="AC16O9_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515957.1"
                     /translation="MQLPARPVEPSSVPEGTDLIEWYYEKGFSDGLPVVPPTPAKVAA
                     MLEALGGEPEKVEARVPPRWGSLTREVLAVNMVMAGCKPEYAPVVRAAMLALCETAFN
                     LNGVQATTHMASPLLVVNGPMARKIGMNAGCNAFGSGNRANATIGRAIRLILLNVGGG
                     WPGDLDKSTLGHPGKYTYAVAENEELGLLAPYHVEKGFRAEDSTVFVMAAEPPHSVTN
                     HVANDPQGILDSIVSAMSTFAHNNAVSSGHCAVVLGPEHATTISKAGWKRHDVKQYLW
                     TNAHNTFGQVAFNHRYGKVYNRSLPVWYG"
     gene            954..2327
                     /gene="selA"
                     /locus_tag="AC16O9_04745"
     CDS             954..2327
                     /gene="selA"
                     /locus_tag="AC16O9_04745"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR6515958.1"
                     /translation="MRLAAIPSVDRILQDPRLAPAIGRHGRPLVVGELRALLAEYRSS
                     LKAGAALPPTGDAIVLGLLNRLDAVAQPSLRRVFNLTGTVLHTNLGRALLPPEAIEAG
                     SAALAQPSNLEYDLEAGERGERDAHLESLLCRITGAEAATAVNNNAGAVLLAMNALAA
                     GREVIVSRGELVEIGGSFRIPEILQAAGCRLREVGTTNRTHLADYEKAIGPATAALLK
                     VHASNYAVVGFTAAPAEGELAALAHAHGLPFVTDLGSGSLVALERYGLPAEPTPMQAL
                     AAGADLVTFSGDKLLGGPQAGLVVGKRERVATLRKNPMKRALRLDKGRIAALEAVLRL
                     HLDPERLRERLPTLRLLTRPAGEIAALAERLRPSVQRYAGDKARVSVEECASQVGSGS
                     LPVDRLPSAALRVAAAAMKPAAIAKALRALPIPVIGRIHDDAVWLDLRCLEDEAAFAH
                     QLEGRAL"
     gene            2372..4030
                     /gene="selB"
                     /locus_tag="AC16O9_04750"
                     /pseudo
     CDS             2372..4030
                     /gene="selB"
                     /locus_tag="AC16O9_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
     gene            4102..4195
                     /locus_tag="AC16O9_04755"
     tRNA            4102..4195
                     /locus_tag="AC16O9_04755"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4136..4138,aa:Sec,seq:tca)
     gene            complement(4223..>5335)
                     /locus_tag="AC16O9_04760"
     CDS             complement(4223..>5335)
                     /locus_tag="AC16O9_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515959.1"
                     /translation="NGVHVDPAQGNWSEGLTTFMADYRYQEERSEQAARDQRLAWLRD
                     FAALPPGEDRPLAQFVARHHGASQIVGYHKAAMTFFMLRELIGEPAFDAGIREFWRAQ
                     RFRAASWGDLQRAFEQTAQRDLAAFFAQWLERTGAPALRLVEARAAQDGASWQVTAML
                     AQDATPYRLRVPLVAHTDQGAHTELVDLRSARESVAFRTTARPRALELDPELRLFRRL
                     APGEAPPILRQAMLDPATAVVLVSGGDAARAARALAGRVLEHAPQVHPATAELPDVPV
                     LAIGLAAEVDAWLAQQGLAPRPPEVAAKGSAQAWAATRRDGRALMVVSARDAAALEAL
                     ARPLPHYGRQSWIAFEGAKAAARGVWPRVPQRVRLD"
BASE COUNT          717 a         1897 c         1948 g          773 t
ORIGIN      
        1 gcccgtacca gaccgggagg ctgcggttgt agaccttgcc gtagcgatgg ttgaacgcga
       61 cctggccgaa cgtgttgtgg gcgttcgtcc acaggtactg cttgacgtcg tggcgcttcc
      121 agccggcttt cgagatggtc gtcgcgtgct cggggccgag caccaccgcg cagtgcccgc
      181 tcgagaccgc gttgttgtgc gcgaaggtgc tcatcgcgga gacgatcgag tcgaggatgc
      241 cctgcggatc gttggccacg tggttggtga cgctgtgcgg cggctccgcc gccatcacga
      301 atacggtgga atcctcggcc ctgaagccct tttccacgtg gtagggcgcg agcagcccca
      361 gctcctcgtt ctcggccacc gcgtaggtgt acttgccggg atggccgagc gtcgatttgt
      421 cgaggtcgcc cggccagccg ccgccgacat tgagcaggat cagccggatc gcgcggccga
      481 tggtggcgtt ggcgcggttg cccgagccga acgcgttgca gccggcgttc atgccgatct
      541 tcctcgccat cgggccgttg acgacgagca gcgggctcgc catgtgcgtg gtcgcctgca
      601 cgccgttcag attgaaggcg gtctcgcaga gcgcgagcat cgccgcgcgc accaccggcg
      661 cgtactcggg cttgcagccc gccatcacca tgttgactgc aaggacttcg cgcgtcaggc
      721 tgccccagcg cggcggcaca cgcgcttcga ctttctccgg ctcgccgcca agcgcctcca
      781 gcatcgcggc gacctttgcg ggcgtgggcg gcacgacggg caggccgtcg gagaagcctt
      841 tctcgtagta ccactcgatg aggtcggtgc cctcgggcac cgaggagggt tcgacgggtc
      901 gggccggaag ctgcatattt cttagaataa ccttgtaacg gtacgatgca cacatgcggc
      961 tcgcagccat cccgtcggtc gaccggatcc tgcaggatcc gcgccttgcc ccggccatcg
     1021 gccggcacgg gcgccccctg gtggtcgggg agttgcgcgc gctgctcgcc gaataccgca
     1081 gttcgctgaa ggccggcgcc gcgctgccgc cgaccgggga cgcgatcgtg ctgggcctgc
     1141 tgaaccgtct cgacgccgtg gcgcagccgt cgctgcgccg cgtgttcaac ctcaccggca
     1201 ccgtgctgca cacgaacctc ggccgcgcgc tgctgccgcc cgaggcgatc gaggcgggca
     1261 gcgcagcgct ggcccagccg tcgaatctgg aatacgacct cgaggcgggc gagcgcggcg
     1321 agcgcgacgc gcacctcgag tcgctcctgt gccgcatcac gggtgccgag gccgccacgg
     1381 cggtgaacaa caacgccggc gccgtgctcc tggcgatgaa cgcgctcgcc gccgggcgcg
     1441 aggtgatcgt gtcgcgcggc gagctggtgg agatcggcgg ctcgttccgc atccccgaga
     1501 tcctccaagc cgcgggctgc cgcctgcgcg aggtcggcac caccaaccgc acgcatctcg
     1561 cggactacga aaaggcgatc gggccggcga cggcggcgct gctcaaggtg cacgccagca
     1621 actacgccgt cgtcggtttc acggccgcgc cggcagaggg cgagctggcc gcgctcgcgc
     1681 acgcgcatgg cctgccattc gtgaccgatt tggggagcgg cagcctcgtt gccctcgagc
     1741 gctacggcct gcccgcggag cccacgccca tgcaggcgct cgcggcgggt gcggatctcg
     1801 tcaccttcag cggcgacaag ctgctcggcg ggccgcaggc gggccttgtc gtcggcaagc
     1861 gcgagcgggt ggcaacgctg cgcaagaacc cgatgaagcg cgcgctccgc ctggacaagg
     1921 gtcgcatcgc ggcgctggaa gcggtgctgc ggctgcacct cgaccccgag cggctgcgcg
     1981 agcggctgcc gacgctgcgg ctgctgacgc ggccggcggg cgagatcgcg gcgctggccg
     2041 aacgcctgcg ccccagcgta cagcgctatg cgggcgacaa ggctcgggtg agcgttgaag
     2101 aatgtgccag ccaggtcggc agcgggtcgc tgcccgtgga tcggctgccg agcgcggcgc
     2161 tgcgcgttgc cgcggcggcg atgaagcccg cagcgatcgc caaggcgcta cgcgcgctgc
     2221 cgatcccggt gatcggccgc atccacgacg acgcggtgtg gctcgacctg cgctgcctcg
     2281 aggacgaggc cgcgtttgcg caccagctgg agggccgcgc gctgtgatcg tcgccaccgc
     2341 cgggcacgtc gatcacggca agaccgcact ggtgcgcgcg ctcacgggcg tcgacaccga
     2401 ccggctgccc gaggaaaagc gccgcggcat gtcgatcgac ctgggctttg cgtacctgcc
     2461 gcagtcgggc ggcgagccca tcgccttcat cgatgttccg gggcacgagc gcttcgtgcg
     2521 caacatgacc gccggcgtca ccgggatcga tcttgcgctg ctcgtcgtcg ccgccgacga
     2581 cggcgtgatg ccgcagacgc gcgagcacct cacgatcctc gagcacctgg gcgcgccgcg
     2641 gctgctggcg gtgctgtcca agatcgatcg cgtggcgccc gagcgcctgc tcgaagccga
     2701 agcggaactg cgcgagcttc ttcccgatac ggaaatcatg aaggtctcgt ccgccaccgg
     2761 cgagggggtg gacttcgtgc gcgggcggct cctggaactg gccagggaga tggcgccgcg
     2821 cgagtcagga cagcgctttc gcatgcacgt cgaccgtgca ttcgtcatcg acggcctcgg
     2881 cctcgtcgtc accggcaccg tggcttcggg cgcgatcgcc gccggggacg acgtgcaggt
     2941 gctgcccgcg ggccgcaagg cgcgcgtgcg cagcctgcgg gcgaatagcg atgcggcgga
     3001 gcgcgcgcgg gcaggccagc gctgcgcgct cgcgctgcac ggcgtctcgc gggacgaagt
     3061 cgggcgcggc gacgtgatcg ccgacgccgg gccggttccc gtgtcgaagc ttctcgacat
     3121 cgaagtagcg tggttcgggg cgccggtcgc gcgtcccaga gcggtcggcg tgcacctggg
     3181 cacggccatg ctccaggcgc gcctgtcgcc gttcgggcgc ttcgcccgtc tcgcctttgc
     3241 gcacggcgtg agcgcatggc aggacgaccg gctgctgctg cgcgacccgg gcacgcagcg
     3301 cctcatcggc gccggcagga tcgtcgatcc gctgccgccc gcacgcggcg cggggcggcg
     3361 cgagcgaatc gcgggcctca acgcggagca agtccgcgac gcgcttctcg cggacggcat
     3421 gctggacgtg gaatggttcg aaaaggcgtt ccatgttccg tttcgcgacg cagcggggct
     3481 gatcaacttc gaagtgcgcg gcgtacgcca cgtcatgcgc gaagagcgct ggcacgcgca
     3541 gtgcaacgcg ctggagcgtg ccgtaggcga gtggcacgcc gcgcatccgg aatcggccgg
     3601 gccgcggccc accgaagccg gcgcgccgct tgtcgccgtg gtcgactttc tcgtcgacgc
     3661 aggtcgcctg gtgcgggacg gcgcgagcct gcgcctgccg ggtcatcgcc cggtgctctc
     3721 gcctcgggac gaagcgctgt ggcagaagtt cgcgccgctc ctcgaaggcg atggcacgaa
     3781 gccgccgcgc gtgcacgagc tggcggcgca acttgcgatg gagccgaagg ccgtgaccga
     3841 ttttctccag cgcgcggcgc gcgccggacg cgtgcatcgc gtcgcgccca accgctattt
     3901 cctgccgcgc gccgtggagc gcctggtcga tctcgcggcg tcgctggacg aggagtcggg
     3961 gcaccacggc tttggcgcgg cgacgttccg cgaccgctgc ggcctcggcc gcaatctcac
     4021 catcgaggtg ctggcgtacc tcgacagctc gggcgatacg cgccgcgcgg gcgatgtgcg
     4081 ccgcgcgcgc aagatttccc tggaagagaa acgtccccgg tggggcgccc ggacttcaaa
     4141 tccggagcga ggcgtctgac gtctcgggtg ggttcgactc ctgcctcttc cgccatgctt
     4201 atcgcggcgc gcgcgggcgc ggctagtcca gcctgacgcg ctgcggcacg cggggccaga
     4261 cgccgcgcgc ggcggccttc gcgccctcga acgcgatcca gctctgccgg ccgtagtgcg
     4321 gcagcgggcg cgcgagcgct tcgagcgccg cggcatcgcg ggctgacacc accatcagcg
     4381 cgcggccatc gcgccgcgtc gccgcccacg cctgcgcgct gcccttggcc gcgacctccg
     4441 gcggccgcgg cgcgagcccc tgctgcgcca gccacgcgtc gacctccgcg gcaagcccga
     4501 ttgccagcac cggcacatcc gggagctccg cggtagccgg gtgaacctgc ggagcatgct
     4561 cgagcacgcg gcccgccagc gcgcgtgcgg cccgcgccgc gtccccgccg gagaccagca
     4621 ccacggcggt tgccgggtcg agcatcgcct ggcgcaggat cggcggggcc tcgccgggcg
     4681 cgaggcgcct gaaaagccgc agctcggggt cgagctcgag cgctcttggc cgcgcggtgg
     4741 tgcgaaaggc gacgctctcg cgtgcgctgc gcaggtcgac gagctccgtg tgcgcgcctt
     4801 gatcggtgtg cgccaccagc ggcacgcgca ggcggtacgg cgtcgcgtcc tgcgccagca
     4861 tcgcggtcac ctgccaggac gcgccgtcct gcgccgcgcg cgcctcgacg aggcgcagtg
     4921 cgggggcgcc cgtgcgctcg agccattgcg cgaagaaggc cgcgagatcg cgctgcgccg
     4981 tctgctcgaa cgcgcgctgc aggtcgcccc aggaggccgc gcggaagcgc tgcgcgcgcc
     5041 agaactcgcg gatgcccgcg tcgaacgccg gctcgccgat cagctcgcgc agcatgaaga
     5101 acgtcatcgc cgccttgtga tagccgacga tctgcgaggc gccgtggtgg cgcgccacga
     5161 actgcgcgag cggccggtcc tcgcccggcg gcagcgcggc gaagtcgcgc agccaggcaa
     5221 gccgctggtc gcgcgccgcc tgctcgctgc gctcctcctg gtaccggtag tcggccatga
     5281 aggtcgtcag gccctcggac cagttgccct gcgcaggatc gacgtgcacc ccgtt
//
LOCUS       DBNHKE010000074         7683 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_435050, whole genome shotgun sequence.
ACCESSION   DBNHKE010000074 DBNHKE010000000
VERSION     DBNHKE010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_435050"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC16O9_04765"
     CDS             <1..300
                     /locus_tag="AC16O9_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515960.1"
                     /translation="RAVITGSLSRLLEATARPPCAGRGARRSSPAAAGPGRRVRGLPC
                     ASRREQAEGAMQASNYWSSSSNNNNNAWNVNFNNGNLNNNNKNNDNHARAVRGGR"
     gene            534..1130
                     /locus_tag="AC16O9_04770"
                     /pseudo
     CDS             534..1130
                     /locus_tag="AC16O9_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012307.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
     gene            complement(1651..2820)
                     /locus_tag="AC16O9_04775"
     CDS             complement(1651..2820)
                     /locus_tag="AC16O9_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6515961.1"
                     /translation="MTPTQPRPARILPVIVAAQFAGASIWFAGNAVLPELQRAWGLPA
                     AALGYVTSAVQLGFIAGTLVFAFLAIADRFSPRLVYLACALAGALANLAAYAADGSLS
                     MLLVTRFATGFFLAGIYPVGMKIASGWFQKDLGHALGLLVGALVLGTAFPHLLRGLGQ
                     AWPWETVMLGVSGCAALGGVLMYALVPDGPHLSRGTKFDPAALATVFRSPPFRASAFG
                     YFGHMWELYAFWAFVPVALAARGAVDNVSLWAFAVIAAGFLGCAVGGRVSLRLGSARV
                     AVAQLGTSGACCLLSPLAFLAPAPVFLAFLLVWGVAVVGDSPQFSALNAANAPRALVG
                     SALTIANCIGFAITVPAIELLNAAAAWLPVQYLFLLLVPGPVLGILAMRPLVGKT"
     gene            2847..3596
                     /locus_tag="AC16O9_04780"
     CDS             2847..3596
                     /locus_tag="AC16O9_04780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR6515962.1"
                     /translation="MTPKHETARAWIGAAERVVVLTGAGISTDSGIPDFRGPQGVWTK
                     NPKAEKLSDIRYYMSDPEVRRLSWQSRLEHSAWRAQPNAGHRALAELEKRGRLHALIT
                     QNIDELHQIAGNSREKVIEVHGTMRQVVCMSCDERAPMERALARVRAGEADPPCRSCG
                     GILKSATISFGQALVPEVIDRAMQAAASADVFLSVGTSLQVYPIAGAVQIAKSAGARL
                     VIINAEPTPFDDLADAVFNESISEVLPRLLG"
     gene            complement(3593..4345)
                     /locus_tag="AC16O9_04785"
     CDS             complement(3593..4345)
                     /locus_tag="AC16O9_04785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6515963.1"
                     /translation="MAAQHKVAVVTGASTGIGEAAGRALVEAGWKVAFAARRAELLEK
                     IVAGCGAANALAVATDVSKPDSVKNLFAKTLEKFGRLDLLFNNAGMGAPPVPMDELPY
                     EKWKEVVDVNLNGMFLCAQEAFRIFRKQIPQGGRIINNGSISAHAPRPFSVAYTSTKH
                     AVTGLTKCIALDGRAYDICGCQIDIGNAATPMTERMTKGVPQANGTTMAEPRMDVQHV
                     ADAIVYMAGLPKESNVLTMTVMATKMPFVGRG"
     gene            4375..4860
                     /locus_tag="AC16O9_04790"
     CDS             4375..4860
                     /locus_tag="AC16O9_04790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6515964.1"
                     /translation="MLPQVRLRRHRPGDLGWVVQRHGELYWKEYGYDERFEALVAAIA
                     ARFVEKFDPRRERCWIAERRGERLGAVLVVKQSPGVAKLRLLIVEPKARGLGLGMRLV
                     KACIGFARAAGYRKLVLWTQSELAAARAIYEKLGFRLVRRERHASFGKRLIGEYWELA
                     L"
     gene            complement(4849..5094)
                     /locus_tag="AC16O9_04795"
     CDS             complement(4849..5094)
                     /locus_tag="AC16O9_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515965.1"
                     /translation="MKPDQLAKAVALLRKGDWEAAHVIVQKDEESPLACWAHGIVHLM
                     EGDTSNARYWYREARREFPAKPSASDEIAALAAALKR"
     gene            complement(5091..5513)
                     /locus_tag="AC16O9_04800"
     CDS             complement(5091..5513)
                     /locus_tag="AC16O9_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6515966.1"
                     /translation="MIPIRDIDHLVLRVRDIEAMRRFYCDVLGATHVAWRPEFGMSHL
                     KVGRSMIDLVTVEGKLGKAGGAAPGREGRNLDHLCLRVEPFDQDAIVAHLERHGIEVG
                     EIRKRYGAEGNGISIYVTDPEGNTVELKGPSDGQPPPS"
     gene            5532..6659
                     /locus_tag="AC16O9_04805"
     CDS             5532..6659
                     /locus_tag="AC16O9_04805"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR6515967.1"
                     /translation="MGSYPYLPLERVVFGQPAAQAAAEEVARIGARKVFVVASRSLSR
                     KTPVVRDIEQALGERYAGRFDECIAHTPWPSVIAAADAVRKAQPELILTVGGGTPIDT
                     VKILQICLAHGITRAEDLEGQGAAKKPSPVRQVAIPTTLSGAEFSNLGGGTDVRSRIK
                     HSFTGPDIGPRSVILDPAITLHTPEWLWLSTGIRGVDHAAEALCSVDAHPYCDGLALH
                     ALRLFAEALPRTQAAPQDLEARLLCQQASWLAASTIARVNYGASHGIGHALGAAADVP
                     HGHTSCIMLPHVLRYNAPATRDRQRLISEALGQAGSPAGDAVAKLVAALGQPSTLRQA
                     GVKREQLPAVAAASMKNRWVLANPQPIRSEQDVMRLLEAAW"
     gene            6656..6952
                     /locus_tag="AC16O9_04810"
     CDS             6656..6952
                     /locus_tag="AC16O9_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6515968.1"
                     /translation="MTYDEVRRSFRWQLPGTFNFAADVVDAWAKDAQKLALLWCDESG
                     RQERYTFARISELSRRLGSVLRARGLRKGDRCIVMLPRLPQWQIAMLRRVLRGR"
     gene            7026..>7683
                     /locus_tag="AC16O9_04815"
     CDS             7026..>7683
                     /locus_tag="AC16O9_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6515969.1"
                     /translation="MTGLTELASLDATAQADLVRRREVTPPELLEAAIARVEKVNPAI
                     NAVVLKMYEQARAAARAPLPAGPLSGVPFLVKDFLAEYAGVPFTEGTAYLGNYVPAED
                     SELVRRYKRAGLVFFGKTNTPELAVGPTTEPRLFGPTRNPWDTGRTAGGSSGGAAAAV
                     AAGMVPMAHGNDAGGSIRVPAACCGVFGLKPSRARVPLGPHYGDLMSGLVAEHALTRS
                     V"
BASE COUNT         1183 a         2665 c         2735 g         1100 t
ORIGIN      
        1 cgcgccgtga tcaccgggtc cctctcccgc ctcctcgagg cgactgccag acccccatgc
       61 gcggggcgag gcgcaaggcg ctcttccccc gccgcggcgg ggccagggcg ccgggtccgc
      121 ggcttgccgt gcgcgagccg tcgggaacaa gcggaggggg caatgcaggc gtccaactac
      181 tggtcgtcca gcagcaacaa caacaacaac gcgtggaacg tcaatttcaa caatggcaac
      241 ctgaacaaca acaacaagaa caacgacaac catgcgcgtg ccgtacgtgg cggccggtga
      301 tcgaccctga cgtattttct ttcgcggcgc tgtggcgctg ctaccggcgc tgccggcgca
      361 acaagcgcaa taccgcgaac gcgctcgcct tcgagctcga tgcggaggcc aagctgctcg
      421 cgctgcagga cgatttgcgc gcgcgctcct accggccggg acgctcgatc tgcttcgtga
      481 ccgacgggcc gaagccgcgc gaggtgttcg cggcggactt tcgcgaccgc gtcgtgcacc
      541 acctgctggt agggcaccag gagcgcgtct tcgagccgat gttcatccac gattccttcg
      601 cctgccgcat cggcaagggc acgctcgccg ccagcgaccg gctgatgcgc tttctcgcgc
      661 aggccacggc gaacggcagg cgagccgcgt gggcgctgaa gctcgacgtg gcgagcttct
      721 ttccctcgat cgacaagccg acgctgtacg agatcatcgc gcggcgcatg gccgatccgg
      781 cgctgctgtg gctcacccgc acgctgctgt tccacgaccc gaccgcggac tacgaattcc
      841 gctcgcgcga cccgcgcgcg ccgggccccg ggagcccggc ctacccgatc ccgccgcaca
      901 agagcctgtt cggcaggcgc gagccgcgcg gcctgccgat cggcaacctg accagccagt
      961 tctggggcaa cgtgtacctg aacgagctgg accagttcgt caagcgcgag ctgaagtgcc
     1021 gccactacct gcgctacgtg gacgacatgg tgctgctggc cgaggacgcg cagacgctgg
     1081 atcgctggtg tgagcaagtt cagtccttcc tgcgcgacag actggagctg cggctgcgcc
     1141 ccgggacgac ctcgccgttt ccggtgcggc aaggcgtcga gttcgtcgga tggaaaacat
     1201 ggtgggacca ccgcctgccg cggctgcgca cgctgggcgc ggtgtccgcg aagctcgagc
     1261 gcttcgagcg ccgcgcggtg cgaccggcgc tcggcggcct ggcggggcga cttgatctag
     1321 ggcagttgca cgggcgcgac gcagtcggac gactttactc gatgcttgct ctacggcccg
     1381 cagcgcctcc tggcggcgcg agtcctcggg ctgcgaaccg ggcgcatcgc gcgcggggga
     1441 ttcgcgctcg ccgcggggtt ccccgcgcgc ctgtgccacc ggtacctcgt tcgggcgatg
     1501 cgccggaacg tcgccgtggc gatggcgccc cgctcggctg cgacgcaaga ccgacccacg
     1561 ctctcgggtc atccaacgag agtgttcgtc ccctcgcgag aggctgcctt cagcaccgcg
     1621 ggctcgtagc cggttggccg acggccgagc tcatgtcttc cccaccaggg gtcgcatcgc
     1681 caggatcccg agcaccggcc cgggcaccag cagcaggaac aggtactgca ccggcagcca
     1741 cgcggcggcg gcgttcagca gctcgatcgc cgggacggtg atggcgaagc cgatgcagtt
     1801 ggcgatggtc agcgccgagc cgacgagcgc gcgcggtgcg ttggcggcgt tcagcgccga
     1861 gaactgcggc gagtcgccga ctaccgcgac gccccagacg agcagaaagg cgagaaacac
     1921 cggcgccggc gcgaggaagg cgagcggcga gagcaggcag caggcgcccg aggtgccgag
     1981 ctgcgccacg gcgacgcgcg cgctgcccag ccgcagcgag acccggccgc ccacggcgca
     2041 gcccaggaac cccgcggcga tgacggcgaa cgcccacagc gagacgttgt ccaccgcgcc
     2101 gcgcgcggca agcgccacgg gcacgaacgc ccagaacgca tacagctccc acatgtggcc
     2161 gaagtagccg aaggcggagg cgcggaacgg cggcgagcgg aacacggtgg cgagcgccgc
     2221 agggtcgaac ttcgttcccc gggagaggtg cgggccgtcg ggcaccagcg cgtacatcag
     2281 gacgcccccg agggccgcgc agcccgatac cccgagcatg accgtctccc agggccacgc
     2341 ctgcccgagg cccctgagca ggtgcggaaa cgcggtgcca aggaccagcg cgcccaccag
     2401 gagcccgagg gcatggccga ggtccttctg gaaccagccc gaggcgatct tcatgcccac
     2461 cgggtagatg ccggcgagaa agaacccggt ggcgaagcgc gtgacgagaa gcatcgagag
     2521 gctgccgtcg gccgcatacg cggcgaggtt ggcgagcgcg cccgcgagcg cgcaggcgag
     2581 gtagaccagc cggggcgaga agcggtcggc gatcgcgagg aacgcgaaca ccagcgtgcc
     2641 ggcgatgaag ccgagctgca ccgcggaggt gacgtagccg agcgccgccg ccggcaggcc
     2701 ccaggcgcgc tgcagctcgg gaagcacggc gttgcccgcg aaccagatcg acgcgccagc
     2761 gaactgcgcc gcgacgatga ccgggaggat gcgcgcgggc cgtggctgcg tgggggtcac
     2821 gcggctgagg ctatcattgg ccctgcatga cgcccaagca cgaaaccgcg cgcgcctgga
     2881 tcggcgcggc cgaacgcgtc gtcgtcctca ccggcgccgg catctccacc gattccggca
     2941 ttcccgactt ccgcgggccg cagggcgtat ggacgaagaa ccccaaggcc gagaagctct
     3001 ccgacatccg ctactacatg agcgacccgg aggtgcggcg cctgtcctgg cagagccggc
     3061 tcgagcacag cgcctggcgc gcgcagccca acgccgggca ccgcgccctg gccgagctgg
     3121 aaaagcgcgg ccggctgcac gcgctcatca cgcagaacat cgacgagctg caccagatcg
     3181 ccggcaactc gcgcgagaag gtgatcgagg tgcacggcac catgcgccag gtggtgtgca
     3241 tgagctgcga cgagcgcgcg ccgatggagc gggcgctcgc gcgcgtgcgc gcgggggagg
     3301 ccgacccgcc gtgccgcagc tgcggcggaa tcctcaagag cgccaccatc tccttcggcc
     3361 aggcgctggt gcccgaggtg atcgaccgcg ccatgcaggc ggcggccagc gccgacgtgt
     3421 tcctgtcggt cggcaccagc ctgcaggtct atccgatcgc cggcgcggtg cagatcgcga
     3481 agagcgccgg cgcgcggctg gtcatcatca acgccgagcc cacgccgttc gacgatttgg
     3541 ccgacgcggt gttcaacgag tcgatcagcg aggtgctgcc gaggctcctc ggctagcccc
     3601 ggcctacgaa gggcatcttg gtcgccatca ccgtcatggt gaggacgttc gattccttcg
     3661 gcagccccgc catgtagacg atggcgtcgg ccacgtgctg cacgtccatg cgcggctcgg
     3721 ccatcgtggt gccgttcgcc tgcggcacgc ccttggtcat cctctcggtc atcggcgtgg
     3781 cggcgttgcc gatgtcgatc tggcagccgc agatgtcgta ggcgcggcca tccagcgcga
     3841 tgcacttggt gaggcccgtc accgcgtgct tggtcgaggt gtaggcgacg gagaagggcc
     3901 gcggcgcgtg cgccgagatc gagccgttgt tgatgatgcg cccgccctgc ggaatctgct
     3961 tccggaagat gcggaacgcc tcctgcgcgc acaggaacat gccgttcagg ttcacgtcca
     4021 ccacttcctt ccacttctcg tagggtagct cgtccatcgg cacgggcggt gcgcccatgc
     4081 cggcgttgtt gaagagcagg tccaggcgcc cgaacttctc cagcgtcttc gcgaagaggt
     4141 tcttcaccga atcgggcttg ctgacgtcgg tagcgaccgc gagcgcgttc gcggcgccgc
     4201 agccggcgac gatcttctcc aggagctcgg cgcgccgcgc ggcgaaggcg accttccagc
     4261 cggcctcgac cagcgcccgg cccgcggcct cgccgatgcc cgtggaggcg ccggtgacca
     4321 cggcgacttt gtgctgtgcg gccatgctaa tttctcctca tggtgaagcg ggcgatgcta
     4381 ccgcaagtgc ggctgcgccg gcaccgcccg ggcgacctcg gctgggtggt gcagcgccac
     4441 ggcgagctgt actggaagga gtacggctac gacgagcgct tcgaggcgct cgttgccgca
     4501 atcgcggcgc ggttcgtgga gaagttcgat ccgcggcgcg agcgctgctg gatcgccgag
     4561 cgcaggggcg aacgtctcgg ggccgtgctg gtggtcaagc agtcgccggg cgtggcgaag
     4621 ctgcggctgc tgatcgtgga gccgaaggcg cgggggctgg gcctgggcat gcgcctggtc
     4681 aaagcgtgca ttggtttcgc ccgcgcggcc ggatacagga aactggtgct gtggacgcag
     4741 agcgaattgg cggccgcgcg ggcgatctat gaaaagcttg ggttccgcct ggtgaggcgc
     4801 gagcggcacg ccagtttcgg gaaacggctc atcggagagt actgggagct agcgctttag
     4861 cgccgcggcg agcgccgcga tttcgtcgga agcagacggc ttcgccggga actcgcgcct
     4921 ggcttcgcgg taccagtagc gcgcattcga cgtatcgccc tccatcaaat gcacgatgcc
     4981 gtgcgcccag caggcgagcg gcgattcctc gtccttctgc acgatcacgt gcgccgcctc
     5041 ccagtcgcct ttgcgcagca gagccaccgc cttggcaagt tggtccggct tcatgacggc
     5101 ggcggttgcc cgtcggaggg gcctttcagc tccaccgtgt tgccctcggg gtcggtgacg
     5161 tagatggaga tgccgttgcc ttccgcgccg taacgcttgc ggatctcgcc gacctcgatg
     5221 ccgtggcgct cgaggtgggc gacgatcgcg tcctggtcga agggctctac gcgcaggcag
     5281 aggtgatcca ggttgcggcc ctcgcggccg ggtgcagcgc cgcccgcttt gccgagcttt
     5341 ccctccaccg tgacgaggtc gatcatcgag cggccgacct tcaggtgcga catgccgaac
     5401 tcgggacgcc aggcgacgtg cgtcgcgccg agcacgtcgc agtagaagcg gcgcatggcc
     5461 tcgatgtcgc gcacgcgcag cacgaggtgg tcgatgtcgc gaatcgggat catggcccgc
     5521 taatatagcg tgtgggcagc tatccctatc ttcccctgga gcgtgtcgtg ttcggccagc
     5581 ccgcggcgca agccgcggcg gaagaagtgg cgcgcatcgg cgcgcgcaag gtgttcgtcg
     5641 tcgcctcgcg gtccctgtcg cgcaagacgc ccgtagtccg cgacatcgag caggcgctcg
     5701 gcgagcgcta tgcgggccgg ttcgacgagt gcatcgcgca cacgccctgg ccgagcgtga
     5761 tcgcggcggc ggacgcggtg cgcaaggcgc agcccgagct gatcctcacg gtgggcggag
     5821 gcacgccgat cgacacggtg aagatcctcc agatctgcct cgcgcacggc atcacgcgcg
     5881 cagaggacct ggaggggcag ggcgcggcga agaagccctc gccggtgcgc caggtcgcca
     5941 tcccgacgac gctctcgggc gccgagtttt ccaacctggg cggcggcacc gacgtgcgct
     6001 cgcgcatcaa gcactcgttc accggtccgg acatcggccc gcgctcggtg atcctcgacc
     6061 cggcgatcac gctgcacacg cccgagtggc tgtggctgtc caccggcatt cgcggcgtcg
     6121 atcacgccgc cgaggcattg tgctcggtgg acgcccatcc gtactgcgac ggactcgcgc
     6181 tgcacgcgct gcggctgttc gccgaggcgc tgccgcgcac ccaggcggcg ccgcaggacc
     6241 tcgaggcgcg cctgctgtgc cagcaggcgt cctggctcgc cgcctccacc atcgcacgcg
     6301 tcaactacgg tgccagccac ggcatcggcc acgcgctcgg cgcggcggcc gacgtgccgc
     6361 acgggcatac gagctgcatc atgctgccgc acgtgctgcg ctacaacgcg ccggcgacgc
     6421 gggacaggca gcgactgatc tcggaagcgc tggggcaggc gggatcgccc gccggcgatg
     6481 cggtcgcgaa gctcgtcgcc gcgcttggcc aaccgtcgac gttgcggcag gcgggcgtaa
     6541 agcgcgagca actgcccgcc gtcgccgccg cgtcgatgaa gaaccgctgg gtgctcgcca
     6601 acccgcagcc gatccgctcg gaacaggacg tgatgcggct gctggaggcc gcctggtgac
     6661 ctacgacgag gtgcgccgtt cgttccgctg gcagctgccc gggacgttca acttcgccgc
     6721 cgacgtcgtc gatgcgtggg caaaggacgc gcagaagctc gcgctcttgt ggtgcgacga
     6781 gtccgggcgg caggagcgct acaccttcgc gaggatttcc gagctgtccc gccggctggg
     6841 cagcgtgctg cgggcccgcg gcctgcgcaa aggcgatcgc tgcatcgtca tgctgccgcg
     6901 cctgccgcag tggcagatcg ccatgctgcg acgggtgctg cgcgggcgct gatcgcgaac
     6961 cgcgatcggc gcgccgggtt gtctatcatt cggcctgctg gtccacgacc ttacggacgg
     7021 atcccatgac cggactgact gaactggctt cactggacgc gacggcccag gcagacctcg
     7081 tccgccggcg cgaggtgacc ccgcccgagc tgctcgaggc cgccatcgcg cgagtggaaa
     7141 aggtgaaccc cgcgatcaac gcggtcgttc tcaagatgta cgagcaggcc cgggccgcgg
     7201 cgcgcgcgcc gctgccggcg ggtccgttgt cgggcgtgcc gttcctggtc aaggatttcc
     7261 tcgccgagta cgccggcgtg ccgttcaccg aaggcacggc ctacctgggc aactacgtcc
     7321 cggccgagga cagcgagctg gtgcgccggt acaagcgcgc ggggctggtc ttcttcggca
     7381 agaccaacac gccggaactc gcggtcggcc cgaccaccga gccgcgcctt ttcgggccga
     7441 cccgcaatcc ctgggacacc ggccgcaccg cgggcggctc gagcggcggc gcggcggccg
     7501 cggttgccgc cggcatggtg ccgatggcgc acggcaacga cgccggcggc tcgatccgcg
     7561 tgcccgccgc gtgctgcggc gtgttcggac tcaagcccag ccgggcgcgc gtgccgctcg
     7621 gcccgcatta cggcgatctg atgagcgggc tggtggccga gcatgccctg acccgctcgg
     7681 tgc
//
LOCUS       DBNHKE010000075         2704 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1229019, whole genome shotgun sequence.
ACCESSION   DBNHKE010000075 DBNHKE010000000
VERSION     DBNHKE010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1229019"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1536
                     /gene="ubiB"
                     /locus_tag="AC16O9_04820"
     CDS             25..1536
                     /gene="ubiB"
                     /locus_tag="AC16O9_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR6515970.1"
                     /translation="MSNLRRLTRIALVALRFGLHEFVPRLAGNPLLRRLSGHADRPRA
                     ERLRRALETLGPIFVKFGQVMSTRRDLLPADLAEELAKLQDRVPPFDPALARAEIERG
                     LGRPVSEVFASLEHEAVASASIAQVHLGVLADGRAVAVKVLRPGVEAAVARDVMLLGT
                     AAWLLERLWADGPRMKPREVVLEFARHLEDELDLMREAANASQLRRNFAGSPLLAIPE
                     VHWEACSTRVMVMERMHGTPVSQVEALRAQGVDIGRLARAGVEIFFTQVFRDGLFHAD
                     MHPGNIMVSTEPETLGRYVALDFGIMGTLTANDKSYLAQNFLAFFNRDYRRVAQAHLD
                     AGWVPPGTRVEAFEAAIRAVCEPVFARPLKEIYFGKLLLRLFQTSRRFHVEIQPQLVM
                     LQKTLLNIEGLGRQLDPDLDLWTTAKPYLERWMSEQIGWRGLVRTIRDEAPFWAATLP
                     QLPRLAHRLLAGDRLEAMERDLAALAETGRRRNLLLAVIAAALVALVLLNLFG"
     gene            complement(1533..2483)
                     /locus_tag="AC16O9_04825"
     CDS             complement(1533..2483)
                     /locus_tag="AC16O9_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HIR6515971.1"
                     /translation="MNTHKNARLTLVRRLEMVRAMLDGGLTPAQAGAEAGVSPPTARK
                     WLGRYLAHGEAGLADRSSRPRLSPRAISPGKALAIIELRRRRLIQTRIGASLGVSKST
                     VGRVLARAGLSRLKDLEPSAPVVRYEHPHPGDLLHIDTKKLGRIERMSHRVTGNRRDG
                     VDGAGWEYLFVAIDDHARIGFTDMYPDERKANAVQFLHNTVAYFASLGVRVRRLLTDN
                     GPAFHSRDFAKACEHLNIKHSFTRPYRPQTNGKAERFIQSALREWAYGIPYNHSTERT
                     QMLDRWIHHYNWHRPHQGIKGLTPVSRLAHSGNNLLTLHS"
     gene            complement(2562..>2704)
                     /locus_tag="AC16O9_04830"
     CDS             complement(2562..>2704)
                     /locus_tag="AC16O9_04830"
                     /inference="COORDINATES: protein motif:HMM:NF021375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HIR6515972.1"
                     /translation="LCLVTGVPGAGKTLVGLDIATKHIDKESALYSVFLSGNGPLVVM
                     AQ"
BASE COUNT          410 a          868 c          944 g          482 t
ORIGIN      
        1 ccagggcggg tgatgacgaa tccgctgtcg aacctgcggc gactgacgcg cattgccctg
       61 gtggcgctgc gcttcggcct gcacgaattc gtccctcggc tggccggcaa tccgctcctg
      121 cgccggctct cgggccacgc cgaccggccg cgcgccgagc gcctgcgccg cgcgctcgag
      181 accctgggcc cgatcttcgt caagttcggg caggtgatgt cgacgcgccg ggacctgctg
      241 cccgcggacc ttgccgagga actggcgaaa ctgcaggacc gcgttccgcc cttcgatccg
      301 gcgctggcgc gcgcggagat cgagcgcgga ctcgggcgtc cggtgtcgga ggtcttcgcc
      361 tcgctcgagc acgaggcggt ggccagcgcc tcgatcgcgc aggtgcacct cggggtgctc
      421 gccgacggcc gcgcggtggc cgtcaaggtg ctgcgccccg gcgtggaagc cgccgtggca
      481 cgcgacgtga tgctgctcgg caccgcggca tggctgctcg agcggctctg ggccgacggg
      541 ccgcgcatga agccgcgcga agtggtgctg gagttcgccc ggcacctgga ggacgaactc
      601 gacctgatgc gcgaggctgc caacgccagc cagctgcgcc gcaatttcgc cggctcgccg
      661 ctgctggcga ttcccgaggt gcactgggag gcctgttcga cgcgggtgat ggtgatggag
      721 cgcatgcacg gcacgccggt gtcgcaggtg gaggcgctgc gcgcgcaggg cgtcgacatc
      781 ggccggctgg cgcgcgccgg cgtcgagatc ttcttcaccc aggtgttccg cgacggcctt
      841 ttccatgccg acatgcatcc gggaaacatc atggtctcga ccgagccgga gacgctcggc
      901 cggtacgtgg cgctcgattt cggcatcatg ggaacgctca ccgcgaacga caagagctac
      961 ctcgcgcaga acttcctcgc cttcttcaat cgcgattacc ggcgtgtcgc gcaggcgcac
     1021 ctggatgccg gctgggtgcc gccgggcacg cgcgtcgagg ccttcgaggc cgcgatccgg
     1081 gcggtctgcg aaccggtgtt cgcgcggccg ctgaaggaga tctatttcgg caagctgttg
     1141 ctgcggctgt tccagacctc gcgccgcttt cacgtcgaga tccagccgca gctggtgatg
     1201 ctgcagaaga ccctgctcaa catcgagggc ctgggtcgcc agctcgaccc ggacctcgac
     1261 ctgtggacca cggcgaagcc ctatctggag cgctggatga gcgagcagat cggctggcgc
     1321 gggctggtgc gcaccatccg cgacgaagcg ccgttctggg cggcaaccct gccgcaactg
     1381 ccgcgtctgg cccaccgcct gctggccggg gaccggctcg aggccatgga gcgcgacctt
     1441 gcggcgctgg ccgagacggg gcggcgacgc aacctgctgc tggcggtgat cgcggcggcg
     1501 ctcgtcgcgc tggtcctgct gaacctgttc ggctagctgt gaagcgtcaa gaggttgttt
     1561 ccggaatgag cgaggcgact gactggggtt aagcctttga tgccttggtg ggggcgatgc
     1621 cagttgtagt ggtgaatcca gcgatcgagc atctgggttc gctccgtgga gtggttgtag
     1681 gggatgccgt aggcccattc gcgcagggcc gactggatga agcgctcggc cttgccgttg
     1741 gtctggggcc tgtagggtcg tgtgaagctg tgcttgatgt tcaggtgctc gcaggccttg
     1801 gcgaagtccc ttgagtggaa ggcagggccg ttgtcggtga gtagacggcg cacccgcacc
     1861 ccgagcgagg cgaagtacgc cacggtgttg tgcaggaact gcacggcgtt ggccttgcgt
     1921 tcatcgggat acatgtcggt gaagccgatc cgggcatgat cgtcgatcgc gacgaacagg
     1981 tactcccagc cggccccatc tacgccgtca cgccgattgc cggtgacgcg atggctcatg
     2041 cgctcgatgc gcccgagctt cttggtgtcg atatgcagca gatcgcctgg gtgcgggtgt
     2101 tcgtagcgca ccacaggggc cgagggctca aggtccttga gccgggagag ccctgctcgg
     2161 gccagcaccc ggcctacggt gctcttggac acgcccaagc ttgcgccaat gcgcgtctgg
     2221 atcaggcgcc ggcggcgcaa ctctatgatc gccaacgcct tgcccgggga gatcgcacgc
     2281 ggactgagcc gtggtcgcga cgagcgatct gcaagcccag cttcgccgtg ggccaggtac
     2341 cgcccgagcc acttgcgcgc ggtgggcggg ctgacgccgg cttcggctcc cgcctgcgcc
     2401 ggcgtcagcc cgccatcgag catggctctg accatctcca atcgacgaac cagtgtgagt
     2461 cgggcattct tatgcgtgtt cattcggctg agctcctgcg cgatttgggg tgggtggtac
     2521 ctcccagttt cgcaaactca gtccggatga acagaaacaa cctattgggc catcacaact
     2581 agaggtccat ttcccgacag aaacacgctg taaagggcac tttccttgtc gatatgctta
     2641 gttgcgatat caagtccaac gagcgtctta cccgcacccg ggacaccggt tactaggcaa
     2701 agcg
//
LOCUS       DBNHKE010000076         4126 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_753536, whole genome shotgun sequence.
ACCESSION   DBNHKE010000076 DBNHKE010000000
VERSION     DBNHKE010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_753536"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16O9_04835"
     CDS             complement(<1..274)
                     /locus_tag="AC16O9_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6515973.1"
                     /translation="MHTRPIPASGEALPVIGLGTWQTFDVGGNAAAQAPLKEVLQALF
                     EAGGRVVDSSPMYGSSESVVGELCAQLGICEPLFLATKVWTQGREEG"
     gene            complement(301..1596)
                     /locus_tag="AC16O9_04840"
     CDS             complement(301..1596)
                     /locus_tag="AC16O9_04840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP/NDP exchange transporter"
                     /protein_id="HIR6515974.1"
                     /translation="MLDFLCRVLRAERHELPALAWAFAYFFVLLAGYYVLRPVRDEMG
                     VQAGLKALPWLFSATFAAMLVLVPLYGWLCARLPRARLLPAVYAFFALNLFAFWALLQ
                     SGVPARALAPAFFVWVSVFNLFVVSVFWSFMADLFDARQAARLYGAIAAGGSCGAIAG
                     PAISALAAAPLGTANLMLVAAAFLGAAIVCVARLGHWSRRHPRAGEPKPEAPLGGSVL
                     AGARAALTSPYLLAICGYLLCYTTLSTALYFQQIAIVGAEIADAAARTRFFATLDLAV
                     NSLTLILQLLAFARLGALLGAAWMLALLPLVSIAGFAWLAAAPGLAALAVFSVARRAC
                     NFALSRPVREALYTVATREERYKAKAFIDTVVYRGGDALAAWLLGALRAAGLGFAAVS
                     LAMLPVGAAWLGLAFFLGARMKAWTPAGAHSSAPSPASR"
     gene            1642..2235
                     /locus_tag="AC16O9_04845"
     CDS             1642..2235
                     /locus_tag="AC16O9_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6515975.1"
                     /translation="MSIGGWIFLALVVALVVWVIAIYNGLVTLRNRFKNAFAQIDVQL
                     KRRYDLIPNLVEAAKGYLQHERQTLEAVIAARGAAVSAAQRAAAAPGDPAAMSGLAQA
                     EGVLGGALGRLLAVFEAYPDLKANQNVLQVQEELTSTENKVAFARQGYNDAVMTYNTK
                     RESFPDNIVAGLFAFKEAMLLEATESKEERQAPKVKF"
     gene            2242..4059
                     /locus_tag="AC16O9_04850"
     CDS             2242..4059
                     /locus_tag="AC16O9_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6515976.1"
                     /translation="MDFFQHQELARRNTRVLVLLYALAVIAVVAAVTAVLAGAYLYAR
                     AAEQPGPVSLAAVPPALLWGGALGTLAVILLVTLVQSLRLGGGGEAVAKMAGARPVSP
                     ETTDPLERRLINVVEEMAIAAGVRVPKVYVMAGESGINAFAAGTEVSNAVVAVTRGTL
                     ETLNRDELQGVIGHEFSHILHGDMRLNIRMIGVLAGIVFIGSIGRFAMHATGRGGGRR
                     QGAAAMFAAGLALFIIGYTGLFFARLIKASIARQREFLADASSVQYTRNPDGIAGALD
                     QIRTSARGTLIESRSAEELSHMYFGESVKLWLSGVFATHPPLEERIRRAHPGFEPLRY
                     RSRRAPPAPPPDEDRERRRKAAEGVLATAVLASQAWGRTPQASSALVGTLDAEKVTRA
                     QRLLDSLSDAQREALHHPQRAKAAVLDLLRPRVDGTRSDVALHLPLVDLALPALKQLG
                     ETERREFLAAVEGVIRADQRVSLHEFVLLTLLWHQLLPGAPVAGSKRIEALREESTTL
                     LSLVAHAGHADPAAAFAAGTAQLGLRESQLLPRERITPEAAGDALDRLRELAPLAKAL
                     LIQAMFAAATADGTIQLGEAELLRVTGAVLDCPLPPLLD"
BASE COUNT          637 a         1452 c         1517 g          520 t
ORIGIN      
        1 tgccctcctc gcgcccctgc gtccagacct tggtggcgag aaacagcggc tcgcagatgc
       61 cgagctgggc gcagagctcg ccgaccaccg actcgctcga gccgtacatg ggcgaggagt
      121 cgaccacccg cccgcccgcc tcgaacagcg cctgcagcac ttccttgagc ggcgcctgcg
      181 ccgcggcatt gccgccgacg tcgaaggtct gccaggtgcc cagaccgatg acgggcagcg
      241 cttcgccgct tgcggggatg ggtcgtgtgt gcatgcgctc tgcccctcgc gctgcccgcg
      301 tcaccgcgag gccggcgaag gcgccgagga gtgcgcgccg gcgggcgtcc atgccttcat
      361 tctagcgccg aggaaaaagg cgaggccgag ccaggcggcg ccgaccggca gcatcgcgag
      421 cgacaccgcg gcgaagccca ggcccgcggc gcgcagcgcg ccgagcagcc acgcggcgag
      481 cgcgtcgccg ccgcgataca ccacggtgtc gatgaacgct ttggccttgt agcgctcctc
      541 gcgcgtcgcc acggtgtaga gcgcctcgcg caccggccgg gacagcgcga agttgcacgc
      601 gcggcgcgcg acgctgaaca ccgcgagcgc cgcaagcccg ggcgcggcgg cgagccaggc
      661 gaagccggcg atggagacga gcggcagcag cgccagcatc cacgccgcgc cgagcagggc
      721 gccgagccgc gcgaaggcga gcagctgcag gatgagcgtg agcgagttca ccgccaggtc
      781 gagcgtggcg aaaaagcgcg tgcgcgcggc cgcatcggcg atctcggcgc cgacgatggc
      841 gatctgctgg aagtagagcg ccgtggagag cgtcgtatag cacagcaggt agccgcagat
      901 ggcgaggagg tagggcgagg tgagcgcggc gcgcgcgccg gcgagcaccg agccgccgag
      961 cggcgcctcc ggcttcggct cgcccgcgcg gggatgcctg cgcgaccagt ggccgagccg
     1021 cgcgacgcag acgatcgccg cgccgaggaa agcggccgcg accagcatca agttggcggt
     1081 gccgagcggc gccgcggcga gtgcgctgat cgcggggccg gcgatggcgc cgcagctgcc
     1141 gcccgcggcg atcgcgccgt acagtcgcgc ggcttgccgt gcgtcgaaca ggtccgccat
     1201 gaagctccag aacaccgaca ccacgaacag gttgaatacg ctcacccaga cgaagaacgc
     1261 cggggcgagc gcgcgcgcgg gcacgccgga ctgcagcagg gcccagaacg cgaacaggtt
     1321 cagggcgaag aatgcgtaca ccgcgggcag cagccgcgcg cgcggcaggc gcgcgcatag
     1381 ccagccgtag agcggcacca gcacgagcat cgcggcaaag gtggcgctga acagccacgg
     1441 cagcgccttg aggccggcct gcacgcccat ctcgtcgcgc accgggcgca gcacgtaata
     1501 gccggcgagc agcacgaaga agtacgcgaa ggcccaggcg agcgccggca gctcgtggcg
     1561 ctcggcgcgc agcacgcggc acagaaaatc gagcatgcgc tatagtagcc gaggcagctt
     1621 cacgcgaagg aggaaggcgc aatgagcatc ggtggctgga tctttctcgc gctcgtcgtc
     1681 gcgctcgtgg tctgggtgat cgcgatctac aacgggctgg tgaccctgcg caaccgcttc
     1741 aagaacgctt tcgcgcagat cgacgtgcag ttgaagcgcc gctacgacct gattcccaac
     1801 ctggtcgagg cggcgaaggg ctacctgcag cacgagcgcc agacgctgga agcggtgatc
     1861 gcggcgcgcg gcgcggcggt gagcgcggcg cagcgcgcag ccgcggcccc cggcgacccg
     1921 gcggcgatga gcggccttgc ccaggccgaa ggcgtcctgg gcggcgcgct cgggcggctg
     1981 ctcgcggtat tcgaggccta tcccgacctg aaggccaacc agaacgtcct gcaggtgcag
     2041 gaggagctca ccagcaccga gaacaaggtg gccttcgcgc gccagggcta caacgacgcc
     2101 gtgatgacct acaacaccaa gcgcgagtcg ttccccgaca acatcgtcgc cggcctgttc
     2161 gcgttcaagg aagcgatgct gctcgaagcc accgagtcca aggaggagcg gcaggcgccc
     2221 aaggtgaagt tctagagtcc cttggatttc ttccagcacc aggagctcgc gcgccgcaat
     2281 acgcgcgtcc tggtgctgct ctatgcgctg gcggtgatcg cggtggtggc cgcggtcacc
     2341 gcggtgctcg ccggcgccta cctttacgcg cgcgccgccg agcagcccgg ccccgtgtcg
     2401 ctcgccgcgg tgccgccggc gcttctctgg ggcggcgcac tcggcacgct ggccgtcatc
     2461 ctgctcgtca cgctggtgca aagcctgcgt ctcggcggcg gcggcgaggc ggtggcgaag
     2521 atggccggcg cgcggccggt gtcgcccgag acgacggacc cgctcgagcg gcgcctgatc
     2581 aacgtcgtgg aggaaatggc gatcgccgcc ggcgtgcgcg tgccgaaggt ctacgtgatg
     2641 gcgggcgagt ccggcatcaa cgccttcgcc gccggcaccg aagtatcgaa cgcggtcgtg
     2701 gcggtgacgc gcgggacgct ggagacgctc aaccgcgacg agctgcaggg cgtcatcggg
     2761 cacgagttca gccacatcct gcatggcgac atgcggctca acatccgcat gatcggcgtg
     2821 ctcgccggca tcgtgttcat cggctcgatc ggccgcttcg ccatgcacgc cacgggccgc
     2881 ggcggcgggc gccggcaggg tgcggcggcg atgttcgcgg cgggcctcgc gctgttcatc
     2941 atcggctaca cgggcctgtt cttcgcgcgg ctgatcaagg cttccatcgc gcgccagcgc
     3001 gagttcctgg ccgacgcgtc cagcgtgcag tacacgagaa accccgacgg catcgccggc
     3061 gcgctggacc agatccgcac gtccgcgcgc ggcacgctga tcgagagccg cagcgccgag
     3121 gagctctcgc acatgtactt cggcgagagc gtgaagctgt ggctctccgg ggtcttcgcc
     3181 acgcacccgc cgctggagga gcgcatccgg cgcgcgcacc ccgggttcga gccgttgcgg
     3241 taccgctcgc ggcgcgcgcc gccggcgccg ccgcccgacg aggaccgcga gcgccggcgc
     3301 aaagcggccg aaggcgtgct ggccactgcg gtactcgcct cgcaggcctg gggccgcacc
     3361 ccgcaggcga gctcggcgct ggtgggcacg ctcgatgcgg aaaaggtgac ccgggcgcag
     3421 cgcctgctcg actcgctctc cgacgcgcag cgcgaggcgc tgcaccaccc gcagagggcg
     3481 aaggcggcgg tgctcgatct gctgcgcccg cgcgtcgacg gcacgcgttc cgacgttgcg
     3541 ctgcacctgc cgctcgtcga cctggcgctg cccgcgctga agcagctggg cgagaccgag
     3601 cgaagggagt tcctcgccgc ggtggaaggc gtgatccgcg ccgaccagcg cgtgtcgctg
     3661 cacgagttcg tgctgctgac gctgctctgg caccagctcc tgccgggcgc gccggtggcg
     3721 ggcagcaagc gcatcgaggc gctgcgcgag gagtcgacca ccctgctctc gctcgtcgcc
     3781 cacgccggcc acgccgaccc ggcggccgcg tttgccgcgg gcacggcgca gctcggcttg
     3841 cgcgagtcgc agctgctgcc gcgggagcgc attacccctg aggcggccgg cgatgcgctg
     3901 gaccggctgc gcgagctcgc gccgctcgcc aaggcgctgc tgatacaggc catgttcgcc
     3961 gccgcgaccg cggacggcac cattcagctc ggcgaggcgg agctcctgcg cgtcaccggc
     4021 gcggtgctcg actgcccgct gccgccgctg ctcgactagc gggcatggat ttcttcgcgc
     4081 agcaggacga ggcgaggcgc gcgtcgcgct ggctggtgtt gtggta
//
LOCUS       DBNHKE010000077         8360 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_806227, whole genome shotgun sequence.
ACCESSION   DBNHKE010000077 DBNHKE010000000
VERSION     DBNHKE010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_806227"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="AC16O9_04855"
     CDS             complement(<1..1951)
                     /locus_tag="AC16O9_04855"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6515977.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLVDLDIESASREQRKSNIYKG
                     IITRIEPSLEAAFIDYGEERHGFLPFKEIDKAYFRAGVDVGRARIQDALEEGQDLIVQ
                     VEKDERGTKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDTLDKLQVP
                     AGMSVIARTAAIGRSPEELQWDLNYLLQLWSAVEGAARTQEKKPFLIYLESSLVIRAI
                     RDYFQPDIGEILIDTEEIYEQARAFMQTVMPANVAKVKLYRDDVPLFSRFQIEHQIES
                     AYSRHVNLPSGGAIVIDHTEAMVAVDVNSARATKGGDIEETALRTNLEAADEVARQLR
                     LRDLGGLIVVDFIDMESQKNQREVENRFREALHYDRARVQVGKISRFGLLELSRQRLQ
                     ASLEETAHINCPRCSGTGFIRGTESTALHVLRIVQEEAMKENTGAVHVQVPVDAASFL
                     LNEKRAEIQKLEARLKVNIVLVPNPHLETPHYKVQRLKHDELNAMEHVPASYEMVEQP
                     EEPKSPDAAAAEKRERPEAAVKAIVPDQPAPNVPEVQFQPPARAQTAPAAVAGTAVAP
                     GFLGRILGWFKSGKPESAAAAANDAAVRPAREPRREQPQRERRPDQRHGGGERRDQRR
                     DRPRDEHRGGERRPQHGGERRPDQRH"
     gene            2219..3169
                     /locus_tag="AC16O9_04860"
     CDS             2219..3169
                     /locus_tag="AC16O9_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6515978.1"
                     /translation="MKDLSKARATLLEVGEEGDAQRIDNYLLRRLKGVPKSHVYRVLR
                     SGEVRVNSGRVKPDYRLRIGDKVRVPPVRLASPGAKPAPRPLALPVVYEDDALLVVDK
                     PSGVAVHGGSGVSYGVIESLRAERPRAKLLELAHRLDRDTSGLLVIAKKRSALVELHR
                     MLREGEAEKVYLALVKGAWAGGARELRERLHKYVAAGGERRVAVHAGGQAAATLVRPV
                     AASDACSLLELRLLTGRTHQIRVHLAHAGHPVLGDDKYGDFELNRALAQHGVKRLMLH
                     ARRLAFAHPLTGARLRLEAPLPADLRRYAEAQLALQPDRL"
     gene            3144..3794
                     /locus_tag="AC16O9_04865"
     CDS             3144..3794
                     /locus_tag="AC16O9_04865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6515979.1"
                     /translation="MRYSLIVFDWDGTLLDSAAGIVESIQEAARDLQLPVPTREAASH
                     VIGLGLQDSLRSAVPTLPEARYREFADAYRRHFLARQDAMDLFPGARELLRDLHAAGH
                     RLAVATGKSRRGLDHALAATSLRPYFRATRCADETQPKPHPAMLLELMAELGASPGHA
                     LMIGDTSHDLQMARSASVDAVAVCYGAHPAPALQALAPRACVASVQELRAWLTTNA"
     gene            3773..4129
                     /locus_tag="AC16O9_04870"
     CDS             3773..4129
                     /locus_tag="AC16O9_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6515980.1"
                     /translation="MADDERLICEATALRDSGDGVRFEVEYFGERAPAFAVRYQGRVH
                     AYLNRCAHVAMELDWREGQFFDFEGRDLICSTHGAVYAAASGQCLGGPCNGAPLVRLR
                     VEERDGRVYYLGVQDG"
     gene            4122..5063
                     /locus_tag="AC16O9_04875"
     CDS             4122..5063
                     /locus_tag="AC16O9_04875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR6515981.1"
                     /translation="MADNDGQWERAAIERLAGAALQEQRRARRWGIFFKLLTFAYITV
                     IILMAMDWGDRRDTKGKHTALVEVNGVIAPGTDASAERVMAALQAAFKDENTQGVVVR
                     INSPGGSPVQSNYIYEEMRRLRKKHPAVPLYAVVEDLCASGGYYVAAGADRIYVGRSS
                     IVGSIGVRMDSFGVTGLMDKLGVERRLLTAGDNKGFLDPFLPVDPAQKAHAQALLEEV
                     HRHFIDAVRDGRGQRLKESPEMFSGLIWTGQKSVELGLADALGSLDYVAREVVKAEKV
                     VDYTQKENLAEKFARRLGAGAANALLEFALRGAAQPR"
     gene            complement(5060..5746)
                     /locus_tag="AC16O9_04880"
     CDS             complement(5060..5746)
                     /locus_tag="AC16O9_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6515982.1"
                     /translation="MTPALYLVPATLGGAPGQVLPAPTIETVRALRDFAVEDAKSARA
                     FLAALGMSCPMRELRIETLDADPAALLAPLRAGRSLGVLSEAGCPGVADPGQALVAAA
                     HAAGFRVVPLVGPSSILLALMASGLEGQRFAFRGYLPRDARERATRIGALEQVSREAR
                     ETQIFIETPYRNDALLAALLEACAPGTRLCVAVDLTLPEESITTRSVLQWREAARVIG
                     RRPAVFLLLA"
     gene            complement(5743..6336)
                     /locus_tag="AC16O9_04885"
     CDS             complement(5743..6336)
                     /locus_tag="AC16O9_04885"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6515983.1"
                     /translation="MPDSRALLLASTSRYRKELLQRLGLPFACVDPQVTEDAIAGEAP
                     AATARRLAAAKARAVAARHGDALVIGADQVASCGDARLDKPGSHANAVRQLRAVSGKS
                     ARFDTAVALLDARSGVLLERVVPSVVHFRDLAPAQIEDYLRREQPYDCAGSAKAEGLG
                     IALIARIDSEDPTALIGLPLIALSELLLQAGMAVLRA"
     gene            6386..6895
                     /locus_tag="AC16O9_04890"
     CDS             6386..6895
                     /locus_tag="AC16O9_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR6515984.1"
                     /translation="MPQPPRFPLIDGFEFAGAGGSMRGAWTAEEFPRLRALLHDTTGS
                     VEYELQGARDELGRHSLALHVAARLRLTCKRCLEAVDVALREDVTLWLARSQGELDAQ
                     PLTAEGPDGIVAAKDMPVRDLVEDQLLLALPYAPRHEKCSAQGSMAPVMRQTPFAGLR
                     GMLRGRNRH"
     gene            6909..7088
                     /gene="rpmF"
                     /locus_tag="AC16O9_04895"
     CDS             6909..7088
                     /gene="rpmF"
                     /locus_tag="AC16O9_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR6515985.1"
                     /translation="MAVQQNKKSPSRRGMHRAHDFIAKPPLAVEPTTGEVHLRHHVSP
                     TGYYRGKKVAKSRDE"
     gene            7140..8180
                     /gene="plsX"
                     /locus_tag="AC16O9_04900"
     CDS             7140..8180
                     /gene="plsX"
                     /locus_tag="AC16O9_04900"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR6515986.1"
                     /translation="MDITVAIDCMGGDHGPRVTVPAALAFQREHAGVRIILVGPHEAL
                     EPALRAQGVTTSPQLRVQHASQLVSMDEPPAQALRLKKDSSMRVAINLVKSGAAHACV
                     SAGNTGALMAISRFVLKTLPGIDRPAIATVLPNMRGEWTYVLDLGANVDCTAEQLMQF
                     GVMGAMLVAAVEHKERPSVGLLNIGVEDIKGNETVKVAADLLRESGLNFHGNVEGDDI
                     YKGTTDVVVCDGFVGNSVLKASEGVANMIVGFLRQEFTRTPWRKLSALLARPVLKALR
                     ARMDPGRYNGASLLGLRGIVIKSHGSADAFAFGKALERAVEEVRNDVIERITRRMAQV
                     HPAAPAARVGAE"
     gene            8177..>8360
                     /locus_tag="AC16O9_04905"
     CDS             8177..>8360
                     /locus_tag="AC16O9_04905"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524529.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HIR6515987.1"
                     /translation="MIYSRIVGTGSYLPPRVVGNEEFAARLDTSDQWIRERTGIVRRH
                     IADESQASSDLALEASR"
BASE COUNT         1245 a         2782 c         3015 g         1318 t
ORIGIN      
        1 catggcgctg gtccggccgg cgctcgccgc cgtgctgcgg ccggcgctcg ccgccgcgat
       61 gctcgtcgcg ggggcggtcg cgccgctgat cccggcgctc gccgccgcca tggcgctggt
      121 ccggccggcg ttcacgctgc ggttgctccc ggcgcggctc ccgcgccggc cgtacggcgg
      181 cgtcgttcgc tgccgccgcc gcgctttcgg gcttgccgga cttgaaccag ccgaggatgc
      241 ggcccaggaa gccgggcgcc acggccgtgc ccgcgaccgc cgcgggtgcg gtctgggcgc
      301 gcgcgggggg ctggaactgc acttcgggca cattcggcgc cggctggtcg ggcacgatcg
      361 ccttgaccgc agcttccggg cgctcgcgct tttccgcggc agccgcgtcg ggcgacttcg
      421 gctcctccgg ctgctcgacc atctcgtagc tcgccggcac gtgctccatg gcgttcagct
      481 cgtcgtgctt gagccgctgc accttgtagt gcggggtttc caggtgcggg ttgggcacca
      541 ggacgatgtt gaccttgagc cgcgcctcga gcttctggat ttcggcgcgc ttttcgttca
      601 gcaggaagct cgccgcgtcc accggcacct gcacgtgcac cgcgccggtg ttctccttca
      661 tcgcttcttc ctgcacgatg cgcagcacgt gcagcgcggt cgattcggtg ccgcggatga
      721 agccggtgcc gctgcagcgc gggcaattga tgtgcgccgt ctcctcgagc gaggcctgca
      781 ggcgctggcg cgagagctcg agcagcccga agcgcgagat cttgcccact tgcacgcgtg
      841 cccggtcgta atgcagggcc tcgcgaaaac ggttctcgac ctcgcgctgg ttcttctgcg
      901 attccatgtc gatgaagtcc accacgatca ggccgcccag gtcgcgcagc cgcagctgcc
      961 gggcgacttc atccgccgcc tcgaggttgg tgcgcagcgc ggtctcctcg atgtcgccgc
     1021 ccttggtggc gcgcgccgag ttgacgtcca ccgcgaccat cgcctcggtg tggtcgatga
     1081 cgatggcgcc gcccgaaggc aggttgacgt ggcgcgaata ggcgctttcg atctggtgct
     1141 cgatctggaa gcgcgaaaaa agcggcacgt cgtcgcgata cagcttcacc ttggcgacgt
     1201 tggccggcat caccgtctgc atgaaggcgc gcgcctgctc gtagatctcc tcggtgtcga
     1261 tgaggatctc gccgatgtcg ggctggaagt agtcgcggat cgcgcggatg acgaggctcg
     1321 actccaggta gatcaggaag ggcttctttt cctgcgtgcg cgcggcgccc tcgacggcgc
     1381 tccacaattg cagcaggtag ttgaggtccc actgcagctc ttcgggcgag cggccgatgg
     1441 ccgccgtgcg cgcgatgacg ctcatgccgg cgggcacctg cagcttgtcc agggtgtcgc
     1501 gcagctcgtt gcggtcttcg ccctcgacgc ggcgcgaaac gccgccgccg cgcgggttgt
     1561 tcggcatcaa tacgaggtag cggccggcaa ggctgatgaa cgtggtcagt gcggcgccct
     1621 tggtgccgcg ctcgtccttc tccacctgga cgatcaggtc ctggccttcc tccagcgcgt
     1681 cctggatgcg ggcgcgcccg acgtcgacgc cggcccggaa ataggccttg tcgatttcct
     1741 tgaagggcag gaacccgtgg cgctcctcgc cgtagtcgat gaaggcggcc tcgaggctgg
     1801 gctcgatgcg ggtgatgatg cccttgtaga tgttgctctt gcgctgctcc cgtgatgccg
     1861 attcgatgtc gagatcgacg agcttctgcc cgtccacgat ggcgacgcgc agctcttcgg
     1921 cctgcgtcgc attaaacaac atgcgtttca cttattgttc tcctgcgcgc gcaggagacc
     1981 ctggtgtccg tcggtcagtg ctctcctagg aaagcggttg cgaaggggcg gggaatgaac
     2041 ctgccgcgcg ctattgagta gtatcgctac ttacggtgag cgatagtcac cactgcttca
     2101 ggtccggctg tggccaaaca ggaggcgggt tgcaggctcc ggcgccggga agttctctcc
     2161 agcacgccgc gcgaacctgt gcctcgcatc tcgaagcaac gggcagatca tacggataat
     2221 gaaagactta agcaaggctc gcgcgaccct actcgaagtc ggggaggaag gcgacgcgca
     2281 gcgcatcgac aattacctcc tgcgccgcct gaagggcgtg ccgaagagcc acgtctatcg
     2341 ggtgctgcgc agcggcgagg tccgcgtgaa cagcggccgc gtcaagcccg actatcggct
     2401 gcgcatcggc gataaggtca gggtgccgcc cgtgcgcctg gcgagcccgg gtgccaagcc
     2461 ggcgccgcga cccctcgcgc tgccggtggt ctacgaggac gatgcgctgc tggtggtgga
     2521 caagccctcg ggtgttgccg tgcacggcgg cagcggggtg agctacggcg tcatcgagtc
     2581 gctgcgcgcg gagcgcccgc gcgccaagct gctcgagctg gcgcaccggc tggatcgcga
     2641 cacctccggg ctgctggtga tcgccaagaa gcgcagcgcc ctcgttgagc tgcaccgcat
     2701 gctgcgcgag ggcgaggccg agaaggtgta cctcgcgctg gtgaagggcg cttgggcggg
     2761 cggcgcacgc gagctgcgcg agcggctgca caagtacgtc gccgccggcg gcgagcgccg
     2821 cgtggcggtg cacgccggcg ggcaggccgc ggcgacgctg gtgcgaccgg tcgccgcgtc
     2881 ggatgcctgc agcctgctcg agctgcgcct gctcaccgga agaacgcacc agatccgggt
     2941 gcacctggcg catgccggcc atcccgtcct gggcgacgac aagtacggcg atttcgagct
     3001 caaccgcgcg ctggcgcagc acggcgtgaa gcggctcatg ctgcacgcgc ggcgcctggc
     3061 attcgcgcac cccttgaccg gtgcgcgcct gcgcctggag gcgccgttgc ctgccgacct
     3121 gcggcgatac gcggaggcgc agcttgcgct acagcctgat cgtctttgac tgggatggga
     3181 cgctgctcga ctcggcggcg ggtatcgtcg agtcgatcca ggaggccgcg cgcgacctgc
     3241 agctccccgt gcccacgcgc gaggccgcca gccacgtcat cggccttggc ctgcaggatt
     3301 cgctgcggtc cgcagtgccg acactgcccg aggcgcgcta ccgcgagttt gccgacgcct
     3361 atcggcggca tttcctggcg cggcaggatg cgatggactt gttccccggc gcgcgtgagc
     3421 tgcttcggga cctgcacgcc gccgggcacc ggctcgcggt ggcgaccggc aagagccggc
     3481 gcggcctcga ccacgcgctg gcggcgacct cgttgcgtcc ttactttcgc gccacgcgct
     3541 gcgccgatga aacgcagccc aagccgcatc cggcgatgtt gctggagctg atggcggagc
     3601 ttggcgcgtc gccgggccac gcgctcatga tcggcgatac ctcgcacgat ctccagatgg
     3661 cgcgcagcgc cagcgtggac gcggtcgcgg tttgctacgg cgcgcacccg gcgccggcgc
     3721 tgcaggcgct ggcgccgcgc gcctgcgtcg ccagcgtcca ggagttgcgt gcatggctga
     3781 cgacgaacgc ctgatctgcg aggcgacggc gctgcgcgat tcgggcgacg gcgtgcgctt
     3841 cgaagtcgag tatttcggcg agcgcgcgcc ggcttttgcc gtgcgctacc aggggcgggt
     3901 gcacgcctac ctcaaccgct gcgcgcacgt cgccatggag ctcgactggc gcgagggcca
     3961 gttcttcgat ttcgaggggc gcgatttgat atgctcgacg cacggcgccg tgtacgcggc
     4021 ggcgagcggc cagtgcctcg gcgggccctg caacggcgcc ccgctggtgc gcctgcgggt
     4081 cgaggagcgc gacggccgcg tctattacct gggagtgcaa gatggctgac aacgacgggc
     4141 aatgggagcg cgccgcgatc gagcggctcg ccggcgcggc gctgcaggag cagcggcgcg
     4201 cgcgccgctg gggcatcttc ttcaagctgc tcaccttcgc ctacatcacg gtgatcatcc
     4261 tgatggcgat ggactggggg gatcgccgcg acaccaaggg caagcacacc gcgctggtcg
     4321 aggtcaacgg cgtgatcgcc cccggcaccg acgccagcgc cgagcgggtc atggcggcac
     4381 tgcaggccgc gttcaaggac gagaacacgc agggcgtcgt ggtgcgcatc aactcgcccg
     4441 gcggcagccc ggtgcagtcg aactatatct acgaggagat gcgccgcctg cgcaagaagc
     4501 atcccgcagt gccgctctac gcggtggtcg aggatctgtg cgcctcgggc ggctattacg
     4561 tggcggcggg tgcggaccgc atctacgtcg gtcgctcgag catcgtcggc tcgatcggcg
     4621 tgcgcatgga cagcttcggc gtcaccggcc tgatggacaa gctcggcgtg gagcggcggc
     4681 tgctcacggc cggcgacaac aagggattcc tggatccgtt cctgccggtg gacccggcgc
     4741 agaaggcgca cgcgcaggcg ctgctcgagg aggtgcaccg gcacttcatc gacgcggtgc
     4801 gtgacgggcg cggccagcgg ctgaaggagt caccggagat gttctccggg ctcatctgga
     4861 ccgggcagaa gagcgtcgag ctcgggctcg cggatgccct cggcagcctc gactacgtgg
     4921 cgcgcgaggt ggtcaaggcg gagaaggtgg tcgactacac gcagaaggag aacctggccg
     4981 agaagttcgc tcggcgcctg ggcgccggcg cggcgaacgc gttgctcgag ttcgcgctgc
     5041 gcggcgcagc gcagccgcgc taggcgagca gcaggaacac cgccgggcgc cggccgatca
     5101 ccctggcggc ctcgcgccac tgcaagacgc tgcgcgtggt gatcgactcc tcgggcagcg
     5161 tcaggtcgac ggccacgcac aggcgtgtgc cgggcgcgca ggcttcgagc agtgcggcga
     5221 gcaaggcatc gttgcggtag ggcgtctcga tgaagatctg cgtctcgcgc gcctcgcgcg
     5281 agacctgctc gagcgcccca atgcgcgtcg cgcgctcccg agcatcgcgc ggcagatagc
     5341 cgcggaacgc gaagcgctgc ccctcgagcc cgctcgccat cagcgcaagc aggatcgagg
     5401 agggcccgac cagcggcacg acgcggaatc cggcggcatg cgcggcagcc accagcgcct
     5461 ggccgggatc ggccacgccg gggcagcccg cttccgacag cacccccagg gaacgcccgg
     5521 cgcgtaacgg cgccagcagc gcagcaggat ccgcatcgag cgtctcgatg cgcagctcgc
     5581 gcatcgggca agacatgccg agcgcggcca ggaacgcgcg ggcgctcttg gcgtcctcga
     5641 ccgcgaagtc gcgcagcgca cggaccgtct cgatcgtcgg cgcaggcagc acctgtcccg
     5701 gcgcaccgcc cagcgtcgca ggcacgagat acaaggcggg cgtcacgcgc gcagcaccgc
     5761 catcccggcc tgcagcagca gctcgctcaa cgcgatcagg ggcaggccga tgagcgcggt
     5821 cggatcttcg ctgtcgatgc gggcgatgag ggcgatgccg agtccttcgg ccttggcact
     5881 gccggcgcag tcgtatggtt gctcgcgacg caggtagtcc tcgatctgcg ccggcgccag
     5941 atcgcggaaa tgcacgacgc tcgggacaac gcgctccagc agcacgccgc tacgcgcgtc
     6001 gagcagggcc accgccgtat cgaagcgcgc cgacttgccg ctcacggcac gcaactgccg
     6061 cactgcgttg gcgtggctgc ccggcttgtc caggcgcgca tcgccgcagg acgccacctg
     6121 gtcggcgccg atgacgagcg cgtcgccgtg gcgcgccgcc acggcacgcg ccttggccgc
     6181 ggccagacgg cgcgccgtcg cggcgggcgc ttctcccgcg atcgcatctt ccgtgacctg
     6241 cggatcgacg caggcgaagg gcagcccgag gcgctgcagc agctccttgc ggtagcgcga
     6301 ggtcgaggcg agcagcagtg cgcgggagtc aggcatgact ttgttttgac acaacttttt
     6361 tcgcgatgat atcatccgcc ccttcatgcc gcagccgccg cggtttccct tgatcgacgg
     6421 cttcgaattc gcgggagccg gcggcagcat gcgcggcgcc tggaccgcgg aggagtttcc
     6481 gcggctgcgc gcgctgctgc atgacacgac gggctcggtc gaatatgaat tgcagggagc
     6541 gcgtgacgag ctcggccgtc acagcctggc gctgcatgtg gccgccaggc tgcgactgac
     6601 ctgcaagcgt tgtcttgagg cggtcgacgt ggcattgcgc gaggatgtca cgctgtggct
     6661 ggcgcgctcg cagggcgagc tcgacgcgca gccgctcacc gcggaagggc ccgacggcat
     6721 cgtcgctgcc aaggacatgc cggtgcggga tctggtggag gatcagctgc tgctggcgct
     6781 gccgtacgcg ccgcggcacg agaaatgttc ggcgcaaggc agcatggcgc cggtgatgcg
     6841 gcagacgccg tttgcaggac tgcgcgggat gctgcgcggc aggaatcgac attgagagga
     6901 gtttgaacat ggctgtgcaa cagaacaaga agtccccgtc gcggcgcggc atgcatcgcg
     6961 cgcatgattt catcgccaag ccgccgctgg cggtggagcc gacgaccggc gaggttcacc
     7021 tgcgtcacca cgtcagcccg accggctatt accgcggcaa gaaggtcgca aagtcgcgcg
     7081 acgagtaacg gcgcgcagcg ctcgcatccc gggtaagcgg cgtaggcgcc gcaggctcga
     7141 tggatatcac tgtcgctatt gactgcatgg ggggggatca cgggccccgt gtgacggttc
     7201 ccgccgcgct ggcattccag cgcgagcacg cgggggtccg gatcatcctg gtggggccgc
     7261 acgaggcgtt ggagccggcg ctgcgcgcgc aaggcgtgac gacgagcccg cagctgcgcg
     7321 tgcagcatgc ttcccagctc gtgagcatgg acgagccgcc cgcgcaggcg ctgcggctga
     7381 agaaagactc ctcgatgcgc gtggcgatca atctggtgaa gagcggggcg gcgcacgcct
     7441 gcgtcagcgc cggcaacacc ggggctctga tggccatctc gcgttttgtg ctgaagacgc
     7501 tgcccggcat cgaccggccc gcgatcgcca cggtcctgcc caacatgcgg ggcgaatgga
     7561 cgtacgtgct cgacttgggc gccaacgtcg actgcacggc ggagcagctg atgcagtttg
     7621 gcgtgatggg cgcgatgctg gtggccgcgg tcgagcacaa ggagcggccc tcggtgggtt
     7681 tgctcaatat cggcgtcgag gatatcaagg gcaatgaaac cgtgaaggtc gcggcagacc
     7741 tgctgcgcga gagcggcctc aatttccacg gcaatgtcga aggcgacgac atctacaagg
     7801 gcaccaccga cgtggtggtg tgcgacggct tcgtcggcaa ctcggtgctc aaggcgtccg
     7861 agggcgtcgc caacatgatc gtcggcttcc tgcgtcagga gttcacgcgc acgccgtggc
     7921 gcaagctctc ggcgctgctg gcgcgtccgg tgctgaaggc gctgagagcc cgcatggatc
     7981 cgggccgcta caacggcgcc agcctgctcg ggctgcgcgg catcgtcatc aagagccatg
     8041 gctcggcgga cgcgtttgcg ttcggcaagg ccctggagcg cgcggtagag gaagtgcgca
     8101 acgatgtgat cgagcgcatc acgcgccgca tggcgcaggt gcatcccgcc gcgccggcgg
     8161 cccgggtggg ggccgaatga tctattcgcg catcgtcggc acgggaagct atttgccgcc
     8221 gcgcgtggtc ggcaacgagg aattcgcggc gcggctcgac accagcgacc agtggattcg
     8281 cgagcgcacc gggatcgtgc ggcgccacat tgccgacgaa tcgcaggctt cgagcgacct
     8341 ggcgctggag gcgagccgcg
//
LOCUS       DBNHKE010000078        25126 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_435849, whole genome shotgun sequence.
ACCESSION   DBNHKE010000078 DBNHKE010000000
VERSION     DBNHKE010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 25126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_435849"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..168)
                     /locus_tag="AC16O9_04910"
     tRNA            complement(92..168)
                     /locus_tag="AC16O9_04910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Arg,seq:ccg)
     gene            216..707
                     /locus_tag="AC16O9_04915"
     CDS             216..707
                     /locus_tag="AC16O9_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6515988.1"
                     /translation="MSTPEEHSSFIKTPQQLIVVVLLAFLVPIIGILLLVNLVLNKSG
                     VDPAALAPQAVAQRLQPVGRIELGATGAGAAASAARTGEEIVNAACGACHVPGVANAP
                     KIGDAAAWGTLAKKGLPEMLKTVIGGKGAMPPRGGVADASDAELARAIEFMASKSGVT
                     LKK"
     gene            741..1685
                     /gene="gshB"
                     /locus_tag="AC16O9_04920"
     CDS             741..1685
                     /gene="gshB"
                     /locus_tag="AC16O9_04920"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR6515989.1"
                     /translation="MNLLFIIDPPEELKAYKDTTVAMMRAAQRRGHQVHVCTQDSLAW
                     RAGPVTARALRIALTDNDEDWFRRDGTAEAALKDFAAVLMRKDPPFDMEYVASTWLLS
                     EAERQGARVYNAPQALRDLNEKFAIAEYAQLTVPSLVTRDMAALHAFVDEHRDTILKP
                     LSGMGGTGIFRVRRDDPNRNVIVETLARHGAESVMAQRFIPEIRAGDKRVIVIEGKPA
                     PYVLARIPKAGETRGNLAVGGKGVAQPLSARDREIAEIMGPPLAARGVLFAGLDVIGD
                     WLTEVNVTSPTCFREIQDQTGYDAGEVFIAALEARLER"
     gene            1682..2086
                     /locus_tag="AC16O9_04925"
     CDS             1682..2086
                     /locus_tag="AC16O9_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR6515990.1"
                     /translation="MIGVLVISHGAIGEALLASAAQILGAPAQAAALGVAPGDAPEAV
                     LARARTLAARLDTGEGLLVLTDMFGATPCNVAARLLADGRVEGVSGVSLPMLVRVLSG
                     RRAALAALVQRALSGGAEGVVHMNSDSCGAKR"
     gene            2073..2342
                     /locus_tag="AC16O9_04930"
     CDS             2073..2342
                     /locus_tag="AC16O9_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR6515991.1"
                     /translation="MPSAELTVTNKLGLHARASAKLTQVASRFAAEVWLTRNGRRVNA
                     KSIMGVMMLAAGRGAALTVEAEGADAEAALAAIRELVAGKFGEGE"
     gene            2347..4071
                     /gene="ptsP"
                     /locus_tag="AC16O9_04935"
     CDS             2347..4071
                     /gene="ptsP"
                     /locus_tag="AC16O9_04935"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6515992.1"
                     /translation="MNFVLHGSSVTSGITIGQAHLVSTARLEVAHYQVPAHAVAGEIF
                     RFDAGIARAKQELAQLESQIPADAPAEFGAFLSLHRMMLEDSSLSHAPREIIRERRCN
                     AEWALVQQMETLEEQFAQVEDAYLRERGADVQQVVERVLKALIGQRAPLAPKLSDEHK
                     LIVVAHDLSPADMLLFKRHEYGGFVTDVGGVTSHTAILARSLNIPALVGLHHARHMIR
                     EGELLIVDGIQGVLVVDPDPLVLAEYRLRQSQHELERKKLGRLKSTPAATLDGTAIEL
                     FANIELPQDLDKVHESGAQGIGLFRTEFLFMNRKDLPGEDEQFEAYREVARKMAGKPV
                     VIRTLDIGADKALSGHIEAMPNPALGLRAIRYCLAEPHIFLAQLRAILRASHHGKVRI
                     LLPMVAHAHEVDQALVLIGQARQQLDERRQPYDKGVEVGAMIEIPAAALALPMFIKRL
                     DFLSLGTNDLIQYTLAIDRTDDAVAHLYDPLHPAVLQLVAQVIHAAARARTPVAVCGE
                     MAGDAALTRLLLGFGLRNFSMHPAQLLAIKERVLRTDLAAAEPLAQKVLRSTDSAKTR
                     ELLAQLNA"
     gene            4084..5277
                     /locus_tag="AC16O9_04940"
     CDS             4084..5277
                     /locus_tag="AC16O9_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6515993.1"
                     /translation="MPGPLDGVRVLDLTTVVMGPYATQILADFGADVVKVEPPEGDIM
                     RWAWPFRSKGMGHIFLNANRNKRGIVLDLKRPEGRDACLALAKAADVLVYNIRPQAMA
                     RLKLGYEDVRAVNPRIIYVGCFGYSQRGPYAAKAAYDDLIQGAAGLPWLSREASGESP
                     RYTPVIIADRSVGLHTANAVSAALYAREKTGKGQRVDVPMFESLLQSVLGEHLGGYTF
                     VPQLGEGGYRRMLTRFRRPYETKDGYVCALIYNDKHWKAFFEMVGRADMVADPRFASA
                     EGRSQNFDAVYSFVADEMARRTTDEWLAALGSADIPVQRMNSLEDILRDPHLEAIGYF
                     TPVEHPSEGSLLSMKVPSEWSETAPAYRRHAPRFGEHTREVLREAGYDDAQIDAMVKS
                     GAARL"
     regulatory      5278..5355
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            5399..6520
                     /gene="metX"
                     /locus_tag="AC16O9_04945"
     CDS             5399..6520
                     /gene="metX"
                     /locus_tag="AC16O9_04945"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HIR6515994.1"
                     /translation="MAGSVGIVEARRAVFEEPFRFRSGAQIPRFELMYETYGTLNAAR
                     SNAVLVCHALNASHHVAGTYADDPGNVGWWDNLVGPGKPLDTERFFVIGNNYIGGCFG
                     STGPTSVNPATGKPYGADFPVVTVEDWVAAQARLADHLGIERFAAVIGGSLGAMQALQ
                     WALAYPERIGHSLVIASTPKLSAQNIAFNEVARQAIVTDPDFHGGHYYEHGVVPARGL
                     RIARMIGHITYLSDDAMMEKFGRQLRRAALGFDFDIDFEIESYLRYQGDKFSTYFDAN
                     TYLRITKALDYYDPAGDFGGDLAKSFERARAAFLVVSFKSDWRFAPARSRAMVKALLD
                     NRRIVSYLELDAPGGHDAFLLEDPRYHGALAAYFDSIDL"
     gene            6517..7095
                     /gene="metW"
                     /locus_tag="AC16O9_04950"
     CDS             6517..7095
                     /gene="metW"
                     /locus_tag="AC16O9_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR6515995.1"
                     /translation="MTRPDLEAIAQWVAPGSRVLDLGCGDGSLLRHLWEQHQAHGYGV
                     EIDDAEVLACLKNDVNVLQLDLESGLALFQGGSFDYVILSETLQTIHRIELLTREMLR
                     VGKEAIVSFPNFGHWRARLQVLAGRMPVSDELPHQWYNTPNVHLCTLHDFEDLCAKLG
                     AKIRERVVLHRGRPVSVLPNLLGSLAVYRVTA"
     gene            complement(7085..7327)
                     /locus_tag="AC16O9_04955"
     CDS             complement(7085..7327)
                     /locus_tag="AC16O9_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6515996.1"
                     /translation="MRKAQCGLGWFGSLIVLALAVGAGYYVYQQLAVPDASPSCEAAL
                     NGCIHKCRQTATDATTIQACQQACREEETVCKSLRR"
     gene            complement(7327..8640)
                     /locus_tag="AC16O9_04960"
     CDS             complement(7327..8640)
                     /locus_tag="AC16O9_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR6515997.1"
                     /translation="MSGRSPSALRPYLEAAPLAALALGISSGFAFAMIGATLATRLAQ
                     HGVTKSAVTAFALTFLAYNLKFLWAPVVDNVRLPLIGRFGQRRSWLWLVGVLVMAAVA
                     FLGVADPEESLLLVAVAAITVGVAGATFDIIIDAYRIELLEPRQLGVGSGMSQYGWRI
                     GAAAAGALALVLAQQLGWGVAYAACAAFALPAMLVGVVMGEPRRHRTPAAPRGALEAV
                     RAYFSPLLEFLQRQGALVVLLFVLIHKIGDTLANLTLRLLFESLGFTNNEIAFYDVGI
                     GFAALLAGIFVGGILYVQLGMKRAVLASLVLMAVSNFSFAALAAVGHTNLGMAAAVGF
                     ENFASGIGGVTVVAYLSALCNLRFTATQYALLSALASIAGRFLTGTTAGSMIEAIGYV
                     NFYLLTTVVAFPGVILFWLMMRSGLADLSVGTAGTVGEGDARGGS"
     gene            complement(8621..9397)
                     /locus_tag="AC16O9_04965"
     CDS             complement(8621..9397)
                     /locus_tag="AC16O9_04965"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6515998.1"
                     /translation="MRVVTLNVNGIRSAAKKGLYRWLAAQKADVACLQEIKAHDGDLL
                     EAMRAPRGLRGFFSHARKKGYAGVALYSRAQPDRIVEGFGVKEFDEEGRCLEARFGKL
                     SVISVYLPSGSAGPHRQASKFRFLDAFLPHLKQLRKSGREVVLCGDWNIAHKEIDLKN
                     WRSNRKNSGFLPEERAWLTRVFDGLGFVDVFRRLDPRPEQYTWWSNRGQAWAKNVGWR
                     IDYQIATPGIAAKATKASIYRRRRFSDHAPLTVDYVWPLA"
     gene            complement(9394..10083)
                     /locus_tag="AC16O9_04970"
     CDS             complement(9394..10083)
                     /locus_tag="AC16O9_04970"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6515999.1"
                     /translation="MHSADRAFIAETTAKMLLEVKAVLFSEGKPFIFTSGWASPVYID
                     CRKLIFYPRIRGQLVDFAVATLAREAGFEQFDVVAGGETAGIPYAAWIADRLGLPMQY
                     IRKKPKGFGRNAQIEGDIREGARTLLVEDLTTDGRSKINFCKALREAGAVVEHVFVNF
                     YYDIFPESKQVMQELGVRLHHLATWWDVLQVVKQGNYLTGAQVGEVEKFLNAPAEWSA
                     AHGGASSIAAS"
     gene            10125..10844
                     /locus_tag="AC16O9_04975"
     CDS             10125..10844
                     /locus_tag="AC16O9_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516000.1"
                     /translation="MIAPWRVAMFRIPLLAACALLIAAWPDATLAQAKSDEATEKARI
                     NALRAQVRSYRCVTKAGRKYYGSTIPPQCTGELVEALSAQGTLLFRIEPPLTPEQRAA
                     KEAEERKAAEAEAARAEARKQAEVQARRDRALLQTYSDESDIERVRERDLASNQEAAA
                     QVQARIAQLRQRQALLAKQSEKYKDEREMPEKHKQDVKAVAYDLSLQEQLLESRKREA
                     AEINARYDEEKRKYRQLTGRR"
     gene            complement(10846..12309)
                     /gene="gatB"
                     /locus_tag="AC16O9_04980"
     CDS             complement(10846..12309)
                     /gene="gatB"
                     /locus_tag="AC16O9_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6516001.1"
                     /translation="MAWEIVVGLETHAQLATQSKMFSGAATAFGAPPNSQACAVDLAL
                     PGVLPVANAAAVALAVKFGLAVGGRINARSVFARKNYFYPDLPKGYQISQYEIPIVAG
                     GALTFAAPGGEKTVRLTRAHLEEDAGKSLHELPHSHRALGHGMSGIDLNRAGTPLLEI
                     VSEPELRGAKEAVAYARALHALVRWIGVCDGNMQEGSFRCDANVSVRRAGDAKLGTRC
                     EIKNLNSFRFMEQAIEFEARRQIALLEDGGAVVQETRLYDPDKGETRSMRTKEEAHDY
                     RYFPDPDLLPLAVSTEDIERLGGQLPELPQAMARRLQSAHGLSEYDARLITASRETAR
                     YFEQVAAGADPKLAANWINGELASRLNEDGIDIGAAFVSAEVLAWMIGRIADGTISNK
                     IAKDKIFPELWRRKNVAVGKAEFEKLIADNAQVSDAGAIERIVDEVLAKNARQVEDYR
                     AGKDKAFNSLIGQVMKATQGKANPAQVNELLKKKLAR"
     gene            complement(12314..13045)
                     /locus_tag="AC16O9_04985"
     CDS             complement(12314..13045)
                     /locus_tag="AC16O9_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516002.1"
                     /translation="MRTLGAVLLMLALTAARADDAGETRELLGMLGGRAALMTLYDNP
                     RPDGSARLTGQYLILPTLLQRFLEGERSKQLGVTFLKEGSSPILYGRPATATLQGTWT
                     GGVFKGTRYGPGGQERERFEFSEKFPDMNRYSAEVRCEPGEGRYSSTLDLAVHEGRLQ
                     RLAWSSRVAPAGHICALKTAEQQPVEGGLRFVAGRCRVTLRDLGELVRVSAEDCAEHC
                     GSLAYLEPVLIDRRGGCQLLRLNSR"
     gene            complement(13042..14502)
                     /gene="gatA"
                     /locus_tag="AC16O9_04990"
     CDS             complement(13042..14502)
                     /gene="gatA"
                     /locus_tag="AC16O9_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6516003.1"
                     /translation="MLDSGLGELQAALAARRVSSVELTRLYLERIRAQNGPLNAFITV
                     DEEVSLEQARAADARRARGEAGPLTGIPVAHKDIFCTRALRTTCGSRMLEHYTSPYDA
                     HVIERFEAAGAVQLGKTNMDEFAMGSSNETSYFGPVRNPWDRTRVPGGSSGGSTAAVA
                     ARMAPVATGTDTGGSIRQPAALSGVCGLKPTYGLVSRYGMVAFASSLDQGGPIAKSAA
                     DLALLLNVMAGHDARDSTSLARPKEDYARSLDLSLKGLRIGLPREYFGEGVEPGVRAC
                     VQAALDWFTQQGARTVPLELPNAALAVPVYYVLAPAEASSNLSRFDGVRYGHRAREFA
                     DLVDMYSRTRAEGFGAEVQRRILVGTYVLSHGYYDAYYLKAQKVRRLIARDFAAAFSQ
                     CDLVMGPTTPTTAFALGAKMDDPVQMYLNDIFTIPAPLAGLPALSVPCGFDGQGLPVG
                     LQLTGSYFAEALLLGAAHQYQRATDWHLRVPKEAPL"
     gene            complement(14503..14790)
                     /gene="gatC"
                     /locus_tag="AC16O9_04995"
     CDS             complement(14503..14790)
                     /gene="gatC"
                     /locus_tag="AC16O9_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6516004.1"
                     /translation="MSLSPDQIRLIARLARIALREDESAAVGERLNRVLAMLDQLKAV
                     DTEGIEPMSHALDVTQPLRADTVTESDQRERFQVAAPAVEDGLYLVPKVIE"
     gene            14933..15976
                     /locus_tag="AC16O9_05000"
     CDS             14933..15976
                     /locus_tag="AC16O9_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6516005.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRQQ
                     GGPNAKKTIQAVGKEAKQMLGKTPGNIVAIRPMKDGVIADFTITEQMLKQFIKKVHDN
                     KLFSPSPRIIICVPCGSTQVERRAIRESAIGAGAGQVYLIEEPMAAAIGADLPVAEAT
                     GSMVVDVGGGTTEVGVISLGGMVYSGSVRVGGDKFDEAIINYIRRNYGMLIGETTAET
                     IKKEIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVGSVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLFMEETGLPVIVADDPLTCVVRGSGKAL
                     EKMEYFGPIFTND"
     gene            16021..16938
                     /gene="mreC"
                     /locus_tag="AC16O9_05005"
     CDS             16021..16938
                     /gene="mreC"
                     /locus_tag="AC16O9_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR6516006.1"
                     /translation="MEHTPPPFFVRGPAPLVRLAFFASLSLTLLVLDARFGYAEAIRS
                     VLTLAAYPIQQLARLPVAAVEGLGGYFASQAHLREENAALRARLLAAALDAQRFESAA
                     AEAANLRRLIGAAERHDRKALPAEILYSGRDPYARKVIVDRGAQHGARPGAPVVDDIG
                     VIGQLTRAHAFVSEVTLLTDKNQAIPVQVLRNGLRAIAFGGGASGLLELRYMAANAEV
                     ESGDLLVTSGIDGTYPPGLPVAEVVRVERDASYTFARVLAQPVAGIERGRFALLLSAG
                     ARPPAYPQDADEKKGLKTRRARKPGAGNG"
     gene            16931..17446
                     /gene="mreD"
                     /locus_tag="AC16O9_05010"
     CDS             16931..17446
                     /gene="mreD"
                     /locus_tag="AC16O9_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR6516007.1"
                     /translation="MAERPQHILMPVRTGVIVASFIGALILDFLPWPDVMLTPDFVAL
                     TLAFWCVRQPRRVGLATGFALGLLVDAGNGVLLGQHALAYSALAFAAIGLSRRILWFD
                     MPGQMLHVLLLLEGAQAVALGVRLAAGAEFPGPTLFIGPAAATLLWPVVSWLLRLPQR
                     RSALLDERRSL"
     gene            17443..19356
                     /gene="mrdA"
                     /locus_tag="AC16O9_05015"
     CDS             17443..19356
                     /gene="mrdA"
                     /locus_tag="AC16O9_05015"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6516008.1"
                     /translation="MRFAELRNTESELRAFQVRLGVAGLAVLIAFALLLARFVWLQVL
                     QHDAYSAKAEENRIAVVPLPPNRGLILDRNGEILARNYLAYTLEIFPAQVRDLEATIA
                     QLAEFVDIQPRDRARFRKLLQETKNAESLPIRTRLSDEEVARIAVNRWRFTGVDIRAR
                     LFRRYPYGELASHVLGYIGRITTSDLEQLQGMQVDANYRGTDYIGKAGIEASYEQELH
                     GTTGFERVEIDAAGRGIRALSRTPAQPGNNVALTLDIRLQEVAERTFGERRGALVAIE
                     PATGGVLAFVSSPGYDPNLFVDGIDPQSWEALMSSPDRPLNNRAIAGLYPPGSTFKPY
                     MALAALELGKRTPRTTISDPGYFVFGDRRFRDSKPGGHGTVDMYKSIVVSSDVYYYML
                     ANDMGIDAIAGFMKRFGFGARTGIDLEGEAEGVLPSPEWKHARFSRPEQQRWYAGETI
                     SIGIGQGYNAYTPVQLAQALATLANNGAMYRPRLVSHIDNPRTGERQYLEPQLVRQVQ
                     LQPEHIAFIKEAMAGVNLEGTGRWAFAGAQYTSGGKTGTAQVVGMKAGEKYEEDKVAE
                     RLRDHSLYIAFAPLEGPKIALAVLVENGGFGARAAAPIARTVLDYYLLGKLPKEPRRA
                     VPEAEVKSDAESD"
     gene            19356..20468
                     /gene="rodA"
                     /locus_tag="AC16O9_05020"
     CDS             19356..20468
                     /gene="rodA"
                     /locus_tag="AC16O9_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR6516009.1"
                     /translation="MEIAGYPLRRLRARVVEGIDAPLLAIAVALSLVGLGVLYSASYE
                     NATRVASQLANLAVALALMWAVAQVAPQTLARLALPAYCAGVALLVAVALGGEVVNGA
                     RRWLNLGVMRFQPSEMMKIALPMMLAWYFHRNEGAIRLRDFAVAGVLLALPVALIARQ
                     PDLGTALLVTAAGFYVIFLAGVSWRLLAGLAVLSIASLFPLWGLLHDYQRRRILTLLD
                     PLQDPLGAGYHTIQSTIAVGSGGIAGKGWLNGSQTHLEFIPERHTDFIFAVFSEEFGL
                     VGNVLLLALYALLVARGLAIAAQAATLFARLLAGAVTLIFFTYAFVNMGMVAGILPVV
                     GVPLPFVSYGGTSLATLFVGVGLLMGIQRHRKLVQT"
     gene            20650..21954
                     /gene="aceA"
                     /locus_tag="AC16O9_05025"
     CDS             20650..21954
                     /gene="aceA"
                     /locus_tag="AC16O9_05025"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR6516010.1"
                     /translation="MQKRDPEIAALNKDWTENPRWQGIERGYTAEDVVRLRGSVKIEH
                     TLARLGAEKLWKLCRERPFVNSLGALTGNQAMQQVKAGVPAIYLSGWQVAADANDSLS
                     MYPDQSLYATTSVPSVVRRINNALMRQDQIQTMEGKGEIDWFAPIVADAESGFGGVLN
                     AFELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVMGV
                     PTVLLARTDAEAADIVTSDVDENDKPFITGERTPEGFYKTKKGFEQALSRGLAYAEYA
                     DMVWCETGTPDLAFARKFAEGIRRKYPGKMLAYNCSPSFNWKKNLDDATIAKFQRELG
                     AMGYKFQFITLAGFHSLNYSMFELAYGYARDNMSAFVALQQKEFAAAANGFTAVKHQR
                     EVGTGYFDEITQVVSGGKASTTALHGSTEDAQFFEERAKKVA"
     gene            21956..23725
                     /gene="aceK"
                     /locus_tag="AC16O9_05030"
     CDS             21956..23725
                     /gene="aceK"
                     /locus_tag="AC16O9_05030"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HIR6516011.1"
                     /translation="MTAPAKVLPIHAARGERAGEVARTILAGFDKHYRLFREASARAK
                     HLFERADWAAMGELARARILMYDRRVQEAVEEVRSHFPEAETDESLWPAIKLAYIPLL
                     HEHRQPECAETFYNSVACQVLHRRYYRNEFIFWRPAVATEHLEGEELAYRSYYPRRKT
                     LRRTLAAIVRGFDLANPWEDLHRDLRSVLRALRARFPRPARARPNLQIQVLGSLFIRN
                     KAAYAVGKIVNGSEEVPFAVPILQNERGELYLDALLLEADQLLVLFSFARAYFFVDME
                     VPAAYVSFLRHLMPRKPRAEMYMAVGLAKQGKTMFYRDLFYHLKHSSDLFTVAPGIKG
                     MVMLVFTLPSFPYVFKVIRDRFAPPKEMDRQTVLDKYLLVKMHDRVGRMADTLEYSLV
                     ALPRERFAPELLEELKAEAASNIEFEDDKVVIKHMYIERRMQPLNLYLEEAIGDGAIE
                     RVRQALHEYGDAIKELAGAGIFPGDMLLKNFGVTRHERVVFYDYDEIAYMTECNFRRI
                     PAPRSFDDEMAAEPYYSIGPHDVFPEQFEKFLVTGAASRALFLERHADLMDPQFWADK
                     QARLRAGVQEDVFPYPQEIRFRR"
     gene            23761..24750
                     /locus_tag="AC16O9_05035"
     CDS             23761..24750
                     /locus_tag="AC16O9_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6516012.1"
                     /translation="MAVSVAMYANSYGRIKDRLDALRLDVDIVTFGLDGKFEIDGARV
                     DPAEVDLDYLWLSPELSLEGGLRTAFEVALRCRSIKVLQTFNAGLDAPAYKQIAAKGV
                     RICNSSAQAIGIAEYVMAQVLALFQPLAEQRELQARRTWQRTPFREIAGTNWLILGFG
                     PIGRAIAQRAKGFAAHVHVVRRTPETGATVDRAGTIADLPTFLPAADVVVLACPLNDA
                     TRGLANKAFFDAIKPGAVLVNIARGGLIEDAALRQALDEGRLAAAVLDVFRTEPLPAD
                     DPLWTHPKIRLTSHTSFSGDGVRRRWEQLFLDNLPRFVRGEALLNEVDPKDLA"
     gene            complement(24747..>25126)
                     /locus_tag="AC16O9_05040"
     CDS             complement(24747..>25126)
                     /locus_tag="AC16O9_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6516013.1"
                     /translation="PLAGLDLATLIAAWLLQALALTLLVAMAGGSGSTLVFVASAFDL
                     VRYSLYILVFAVVVQAVLSWVNPHSPVMYLFDAMTRLFLRPIRRVVPLLGSVDLSPLV
                     LLVLLQVALIALEHLRTAAWSAL"
BASE COUNT         3999 a         8750 c         8553 g         3824 t
ORIGIN      
        1 gccgcgcgca cgctacacac ggcatgcatc accggcactg gattcgcgcg catttcatcg
       61 taacagtgcg cacggatgac gcggaggatg ctggcgcgcc cggcgggatt cgaacccacg
      121 acccttggct tcggaggcca atactctgat ccgctgagct acgggcgctt tcaagctaaa
      181 atcatacctt tataaacgca cgcaggccac gccgcatgag tacgcccgaa gagcattcct
      241 ccttcatcaa gacaccgcag caactgatcg tggtggtgct gctcgcgttt ctggtgccga
      301 tcatcggcat cctgctgctg gtcaatctgg ttctgaacaa atcgggcgtg gatcccgcag
      361 cgctcgcgcc gcaggccgtg gcgcagcgcc tgcagcccgt cggccggatc gagcttggcg
      421 ccacgggcgc cggtgccgcc gcgtccgcgg cccgcaccgg cgaggagatc gtcaacgccg
      481 cctgtggcgc gtgccacgtg ccgggggtcg ccaacgcgcc gaagatcggc gatgcggcgg
      541 cctggggcac gttggcgaag aaggggctgc ccgagatgtt gaagaccgta atcggcggca
      601 agggcgccat gccgccgcgc ggcggcgtcg ccgacgcctc cgacgcggag ctggcgcgcg
      661 ccatcgagtt catggcgagc aagtcgggcg tgaccctcaa gaagtagccg gccggcgcca
      721 gccggcggcg cgcatcgccc gtgaacctgc tcttcatcat cgatccgccg gaagagctca
      781 aggcgtacaa ggacaccacg gtggccatga tgcgcgccgc gcagcgccgc ggccaccagg
      841 tgcacgtctg cacgcaggac tcgctcgcct ggcgcgccgg cccggtcacg gcgcgcgccc
      901 tgcgcatcgc gctcaccgac aacgacgagg actggtttcg ccgcgatggc acggccgagg
      961 ccgcgctcaa ggatttcgcc gcggtgctga tgcgcaagga cccgccgttc gacatggagt
     1021 acgtggcgag cacgtggctg ctgtcggagg ccgagcgcca gggtgcgcgc gtctacaacg
     1081 cgccgcaggc gctcagggac ctgaacgaga agttcgccat cgccgagtac gcgcagctca
     1141 cggtgccctc gctggtcacg cgcgacatgg cggcgctgca cgccttcgtc gacgagcacc
     1201 gcgacacgat cctgaagccg ctctcgggca tgggcggcac gggcatcttc cgcgtccggc
     1261 gcgacgaccc caaccgcaac gtgatcgtgg agaccctggc gcgtcacggc gccgagagcg
     1321 tgatggcgca gcgcttcatc cccgagatcc gcgccggcga caagcgcgtc atcgtcatcg
     1381 aaggcaagcc cgcgccgtac gtgctggcgc gcatcccgaa agcgggcgag acgcgcggca
     1441 acctcgccgt gggcggcaag ggggtggcgc agccgctgtc ggcgcgcgac cgggagatcg
     1501 ccgagatcat gggcccgccg ctcgccgcgc gcggcgtact gttcgcgggc ctggacgtga
     1561 tcggcgactg gctcaccgag gtcaacgtca ccagcccgac gtgctttcgc gagatccagg
     1621 accagaccgg ctacgacgcg ggagaggtgt tcatcgcggc gctggaagcg aggctcgagc
     1681 ggtgatcggc gtcctggtga tctcgcacgg cgccatcggc gaggcgctgc tcgccagcgc
     1741 cgcgcagatc ctcggcgccc ccgcacaggc cgccgcgctc ggcgtggcgc ccggcgacgc
     1801 cccggaggcg gtgctcgcgc gcgcgcgcac gctcgccgcc cgcctggaca cgggcgaggg
     1861 gctgctggtg ctcaccgaca tgttcggcgc cacgccctgc aacgtcgccg cgcggctgct
     1921 ggccgacggc cgcgtggagg gcgtatccgg cgtgtcgctg ccgatgctgg tgcgcgtgct
     1981 ttcgggccgc cgcgccgcgc tcgccgcgct ggtgcagcgc gccctctcgg gcggcgccga
     2041 aggcgtggtg cacatgaact cggattcctg cggtgccaag cgctgagctc accgtcacca
     2101 acaagctcgg gctgcacgcg cgcgcgtcgg ccaagctgac ccaggtggcg agccgcttcg
     2161 ccgccgaggt ctggctgacg cgcaacggcc ggcgcgtcaa cgcgaagagc atcatgggcg
     2221 tcatgatgct cgccgccggg cgcggcgccg cgctcacggt ggaagccgaa ggcgcggacg
     2281 ccgaggccgc gctggcggcg atccgtgagc tggtggccgg caagttcggc gagggggagt
     2341 aggacggtga acttcgttct gcacggctcg agcgtcacct cgggcatcac catcggccag
     2401 gcgcacctcg tgtccacggc acgcctcgaa gtggcgcact accaggtccc ggcgcacgcg
     2461 gtggcgggcg agatcttccg cttcgacgcc ggcatcgcgc gcgccaagca ggagctggcc
     2521 cagctcgaaa gccagatccc ggcagacgcg ccggccgagt tcggcgcctt cctcagcctg
     2581 caccgcatga tgctggagga ctcctcgctg tcgcacgcgc cacgcgagat catccgcgag
     2641 cgccgctgca acgccgagtg ggcgctggtg cagcagatgg agacgctcga agagcagttc
     2701 gcgcaggtcg aagacgccta cctgcgcgag cgcggcgccg acgtgcagca ggtggtcgag
     2761 cgcgtgctga aggcgctgat cggccagcgc gcgccgctcg cgcccaagct cagcgacgag
     2821 cacaagctca tcgtggtggc gcacgacctc tcgcccgccg acatgctgct gttcaagcgc
     2881 cacgagtacg gtggcttcgt caccgacgtc ggcggcgtca cctcgcacac cgcgatcctc
     2941 gcgcgcagcc tcaacatccc ggcgctggtc ggcctgcacc atgcgcgcca catgatccgc
     3001 gagggcgagc tcctgatcgt ggacggcatc cagggcgtgc tggtggtgga ccccgatccc
     3061 ctggtgcttg ccgagtaccg cctgcgccag tcgcagcacg agctggagag gaagaagctc
     3121 gggcgcctga agagcacgcc ggcggccacg ctcgacggca cggcgatcga gcttttcgcc
     3181 aacatcgagc tgccgcagga cctggacaag gtgcacgagt cgggggcgca gggcatcggc
     3241 ctgttccgta ccgagttcct gttcatgaac cgcaaggacc tgccgggcga ggacgagcag
     3301 ttcgaggcct accgcgaggt ggcgcgcaag atggcgggca agcccgtggt gatccgcacg
     3361 ctcgatatcg gcgccgacaa ggcgctttcg ggccacatcg aggcgatgcc caacccggcg
     3421 ctggggctgc gcgccatccg ctactgcctc gccgagccgc acattttcct tgcgcagctg
     3481 cgcgccatcc tgcgcgcctc gcaccacggc aaggtcagga tcctgcttcc gatggtggcg
     3541 cacgcgcacg aggtcgacca ggccctggtg ctcattggcc aggcgcgcca gcagctcgac
     3601 gagcggcgcc agccctacga caagggcgtc gaggtgggcg ccatgatcga gattccggcg
     3661 gcggcgctcg cgctgcccat gttcatcaag cggctggatt tcctctcgct cggcaccaac
     3721 gacctgatcc agtacacgct cgccatcgac cgcaccgacg acgcggtggc gcacctgtac
     3781 gatccgctgc accccgcggt gctgcagctg gtggcgcagg tgatccacgc cgcggcgcgc
     3841 gcgcgcacgc cggtggcggt gtgcggcgag atggcgggcg atgctgcgct cacgcgcctg
     3901 ctgctcggct tcgggttgcg caacttctcc atgcacccgg cgcagctgct cgcgatcaag
     3961 gagcgcgtgc tgcgtaccga cctcgccgcg gccgagccgc tggcgcagaa ggtgctgcgc
     4021 tccaccgact cggcgaagac gcgcgagctt ctggcgcagc tcaacgcctg agaaaggccc
     4081 ccgatgcccg gaccgctgga tggcgtgcgc gtgctggacc tcaccaccgt ggtgatgggc
     4141 ccctacgcca cgcagatcct cgccgacttc ggcgccgacg tggtgaaggt cgagccgccc
     4201 gagggcgaca tcatgcgctg ggcctggccg ttccgctcca agggcatggg gcacatcttc
     4261 ctcaacgcca accgcaacaa gcgcggcatc gtgctcgacc tgaagaggcc cgagggacgc
     4321 gacgcctgcc tcgcgctggc gaaggccgcc gacgtgctcg tgtacaacat ccgtccgcag
     4381 gcgatggcgc gcctgaagct cggctacgag gacgtgcgcg cggtgaaccc ccgcatcatc
     4441 tacgtcggct gcttcggcta cagccagcgc ggcccgtacg ccgccaaggc cgcctacgac
     4501 gatctgatcc agggcgcggc gggcctgccg tggctgtcgc gcgaggcgag cggcgagtcg
     4561 ccgcgctaca cgccggtgat catcgccgac cgctcggtgg gcctgcacac ggcgaacgcg
     4621 gtaagcgcgg cgctctacgc gcgcgagaag accggcaagg gccagcgcgt cgacgtgccg
     4681 atgttcgaga gcctgctgca gtccgtgctc ggcgagcacc tgggcggcta caccttcgtg
     4741 ccgcaactgg gcgagggggg ctaccggcgc atgctcacgc gctttcgccg cccctacgag
     4801 accaaggacg gctacgtctg cgcgctgatc tacaacgaca agcactggaa ggcgttcttc
     4861 gagatggtcg ggcgcgccga catggtggcc gacccgcgct tcgcctccgc ggaggggcgc
     4921 agccagaact tcgacgccgt ctacagcttc gtcgccgacg agatggcccg gcgcacgacc
     4981 gacgagtggc tcgccgcgct tgggtccgcc gacatcccgg tgcagcgcat gaacagcctc
     5041 gaggacatcc tgcgcgatcc gcacctcgag gcgatcggct acttcacgcc cgtcgagcac
     5101 ccgagcgagg gctcgctcct gtcgatgaag gtgccctcgg agtggtcgga gacggcgccc
     5161 gcctaccggc gccacgcacc gcgcttcggc gagcacacgc gcgaggtact gcgcgaggcg
     5221 ggctacgacg acgcgcagat cgacgccatg gtgaaaagcg gggccgcacg gctataatcg
     5281 cgtcccagcg ccgatttgat gttcagcttg cgggcgctct acaaatgccg ctaaagagac
     5341 cggcgaaaaa gcccgttctc gcgaaagctg aacgggcttt ttgcgtttgg gaggcgggat
     5401 ggccggctcg gtcggcatcg tagaggcgag gcgcgcggta ttcgaggagc cgttccggtt
     5461 caggagcggc gcccagatcc cgcgcttcga gctcatgtac gagacctacg gcacgctgaa
     5521 cgccgcgcgc tccaacgcgg tgctcgtgtg ccacgcgctc aacgcctcgc accacgtcgc
     5581 cggcacctat gccgatgatc ccgggaacgt cggctggtgg gacaacctcg tcggccccgg
     5641 caagcccctc gacaccgagc gcttcttcgt catcggcaac aactacatcg gcggctgctt
     5701 cggctccacc ggcccgacct cggtcaaccc ggcgaccggc aagccctacg gcgccgactt
     5761 cccggtggtc acggtggagg actgggtggc ggcgcaggcg cggctcgccg accacctcgg
     5821 catcgagcgc ttcgcggcgg tgatcggcgg gtcgctcggc gccatgcagg cgctgcagtg
     5881 ggcgctcgcg taccccgagc ggatcggcca ctcgctcgtc atcgcctcga cgcccaagct
     5941 ctcggcgcag aacatcgcct tcaacgaggt ggcgcgccag gccatcgtca ccgaccccga
     6001 tttccatggc gggcactact acgagcacgg cgtggtgccg gcgcggggcc tgcgcatcgc
     6061 gcgcatgatc ggccacatca cctacctctc ggacgacgcg atgatggaga agttcgggcg
     6121 ccagctgcgc cgcgccgcgc tcggcttcga cttcgacatc gacttcgaga tcgagagcta
     6181 cctgcggtac cagggcgaca agttctcgac gtacttcgac gccaacacct acctgcggat
     6241 caccaaggcg ctcgactact acgacccggc gggcgacttc ggcggcgacc tggcgaagtc
     6301 cttcgagcgt gcccgggcgg cgtttctcgt cgtctcgttc aagtccgact ggcgcttcgc
     6361 gccggcgcgc tcgcgcgcca tggtgaaagc gctgctcgac aaccggcgca tcgtctccta
     6421 cctcgagctc gacgcccccg gcgggcacga cgcgttcctg ctcgaggacc cgcgctatca
     6481 cggcgcgctc gccgcctact tcgacagcat cgacctatga cgcgccccga cctcgaagcg
     6541 atcgcgcagt gggtggcgcc gggctcgcgc gtgctcgatc taggctgcgg cgacggctcg
     6601 ctgctgcggc acctgtggga gcagcaccag gcgcacggct acggcgtgga gatcgacgac
     6661 gccgaagtgc tcgcctgcct gaagaacgac gtcaacgtcc tgcagctcga cctggaatcg
     6721 ggcctcgcgc tctttcaggg cggctccttc gactacgtca tcctgtccga gacgctgcag
     6781 accatccacc gcatcgagct cctcacccgc gagatgctgc gcgtgggcaa ggaggcgatc
     6841 gtgtcgttcc ctaacttcgg ccactggcgg gcgcggctgc aggtgctcgc gggaaggatg
     6901 cccgtgtccg acgagctgcc gcaccagtgg tacaacacgc cgaacgtgca cctgtgcacg
     6961 ctgcacgact tcgaggactt gtgcgcgaag ctcggcgcga agatccgcga gcgcgtggtg
     7021 ctgcaccgcg ggcggccggt gagcgtgctg ccgaacctgc tcggcagcct ggccgtctac
     7081 cgcgtcaccg cctgaggctc ttgcagaccg tttcttcctc gcggcacgcc tgctggcagg
     7141 cctggatcgt ggtggcgtcg gtcgccgtct gtcggcactt gtggatgcag ccgttgagcg
     7201 ccgcctcgca gctcggcgag gcgtcgggga cggccagctg ctggtacacg tagtatccgg
     7261 cgcccaccgc gagcgccagc acgatcagcg acccgaacca gcccaacccg cattgcgctt
     7321 ttcgcatcag gagcctccgc gcgcgtcgcc ttcgcccacc gtgccggcgg tgccgaccga
     7381 caggtcggcg agtcccgagc gcatcatgag ccagaacagg atcacgcccg ggaacgcgac
     7441 gacggtggtg agcagataga agttcacgta gccgatcgcc tcgatcatcg agccggccgt
     7501 ggtccccgtg aggaagcgcc cggcgatgct cgccagcgca gacagtagcg catactgggt
     7561 cgcggtgaag cgcaggttgc acagcgccga caggtacgcc accaccgtca cgccgccgat
     7621 gccgctggcg aagttctcga agcccaccgc ggccgccatg ccgaggttgg tatgaccgac
     7681 cgccgccagc gcggcgaagc tgaagttcga caccgccatc aggaccaggc tcgccagcac
     7741 ggcgcgcttc atccccagct gcacgtacag gatgccgccg acgaagatgc cggcgagcag
     7801 cgctgcgaag ccgatgccga cgtcgtagaa ggcgatctcg ttgttggtga agccgaggct
     7861 ctcgaacagc agccgcagcg tcaggttggc aagcgtgtcg ccgatcttgt ggatgaggac
     7921 gaacagcagc accacgagcg cgccctggcg ctgcaggaac tccagcagcg ggctgaagta
     7981 ggcgcggacc gcctcgagcg caccgcgcgg tgcggcgggc gtgcggtgcc gccgcggctc
     8041 gcccatcacc acgccgacca gcatcgccgg gagcgcgaag gcggcgcaag cggcgtacgc
     8101 cacgccccaa ccgagctgct gggcgaggac cagcgccagc gcgcccgccg cggcggcgcc
     8161 gatgcgccag ccgtactgcg acatgcccga gccgacgccg agctggcgcg gctcgagcag
     8221 ctcgatgcga taggcgtcga tgatgatgtc gaaagtcgca ccggccacgc cgacagtgat
     8281 cgcggcgacc gccacgagca acagcgactc ctccggatcg gcgacgccga gaaaggccac
     8341 cgccgccatc accagcacgc cgaccagcca cagccagctg cggcgctggc cgaagcggcc
     8401 gatcaacggc aggcgcacat tgtcgacgac gggcgcccac aggaacttca ggttgtaggc
     8461 gagaaacgtg agcgcgaacg cggtgaccgc gctcttggtg acgccgtgct gggcaaggcg
     8521 cgtggcgagg gtcgcgccga tcatcgcgaa cgcgaagccc gaagagatgc cgagagcgag
     8581 ggccgcgagc ggtgcggcct cgaggtaggg ccgcagcgcg ctaggcgagc ggccagacat
     8641 agtcgaccgt cagcggcgca tggtccgaga agcggcgccg cctgtagatc gaagctttcg
     8701 tcgccttggc ggcgatgccc ggtgtggcga tctggtagtc gatgcgccag ccgacgttct
     8761 tcgcccaggc ctgaccgcgg ttcgaccacc aggtgtactg ctcgggccgg ggatcgaggc
     8821 gccggaacac gtcgacgaag ccgagcccgt cgaacactct cgtcagccag gcgcgctctt
     8881 cgggcaggaa gcccgagttc ttccggttgg aacgccagtt cttcaggtcg atttccttgt
     8941 gcgcgatgtt ccagtcgccg cacagcacca cctcgcgccc gctcttgcgc agttgcttca
     9001 ggtgcggcag gaacgcatcg aggaagcgga acttggaggc ctgccggtgc ggccccgcgg
     9061 aacccgaggg caggtagacc gagatcaccg acagcttgcc gaagcgcgcc tcgaggcagc
     9121 gtccctcttc atcaaactcc ttcacgccga agccttcgac gatccggtcg ggctgcgccc
     9181 ggctgtacag cgcgacaccg gcgtagccct tcttcctggc atgcgagaag aaaccgcgca
     9241 gtccgcgcgg cgcgcgcatc gcctcgagca gatcgccgtc gtgcgccttg atctcctgca
     9301 ggcaggcgac gtcggccttc tgcgcggcga gccaacggta caggcccttt ttcgccgccg
     9361 agcggatgcc gttgacgttg agggtgacga ccctcaagac gcagcgatgc tcgaggcccc
     9421 gccgtgcgcg gcggaccact cggccggcgc gttgaggaat ttctcgactt cgcccacctg
     9481 cgcgccggtc aggtagttgc cctgcttgac cacctgcagg acatcccacc aggtcgcgag
     9541 gtggtgcagc cgcacgccga gctcctgcat cacctgcttg gattccggga agatgtcgta
     9601 gtagaagttc acgaacacgt gctcgacgac cgcgcccgcc tcgcgcagcg ccttgcagaa
     9661 attgatcttc gagcgcccgt cggtggtcag gtcctccacc agcagcgtcc tcgcgccctc
     9721 gcggatgtcg ccctcgatct gcgcgttgcg cccgaagccc ttgggcttct tgcggatgta
     9781 ctgcatcggc aggccaagcc ggtccgcgat ccatgccgca tacggaatgc cggcggtctc
     9841 gccgcccgcc accacgtcga actgctcgaa gcccgcctcg cgcgccagcg tcgcgacggc
     9901 gaagtccacc agctggccgc ggatgcgcgg atagaagatg agcttgcggc agtcgatgta
     9961 caccgggctg gcccagcccg aagtgaagat gaagggcttg ccctcggaga acaggaccgc
    10021 cttcacctcg aggagcatct tcgcggtggt ttcggcgatg aacgcgcggt cggcggaatg
    10081 catggtggaa gcgcgcggat tctagcaggg ttagaatccg cgcgatgatc gcaccatgga
    10141 gggtcgcgat gttccgcata cccctgctcg ccgcctgcgc gttgctgatc gctgcgtggc
    10201 ccgacgcgac gttggcgcag gcgaagtccg acgaggccac ggaaaaggcg cgcatcaacg
    10261 ccctgcgcgc gcaggtgcgc tcgtaccgct gtgtgaccaa ggcgggacgc aagtactacg
    10321 gctccacgat cccgccgcag tgcacgggcg agctggtcga ggcgctctcg gcgcagggga
    10381 cgctgctgtt tcgcatcgag cccccgctca cgcccgagca gcgcgcggcg aaggaggccg
    10441 aggagcgcaa ggccgccgag gccgaggccg cgcgcgccga ggcgcggaag caggccgagg
    10501 tgcaggcgcg ccgcgaccgt gccctgctgc agacctactc cgacgagagc gacatcgagc
    10561 gcgtgcgcga gcgcgacctc gccagcaacc aggaggcggc ggcgcaggtc caggcccgca
    10621 ttgcacagct ccggcagcgc caggcgctgc tcgccaagca gtccgagaag tacaaggatg
    10681 agcgcgagat gcccgagaag cacaaacagg acgtcaaagc ggtggcctac gacctttcgc
    10741 tgcaggagca gctgctcgaa tcgcgcaagc gcgaggccgc cgagatcaac gcgcgctacg
    10801 acgaggaaaa gcgcaagtac cgccagctga ccgggcgccg ctagctcagc gcgcgagctt
    10861 cttcttcagc agttcgttca cctgcgcggg gttggccttg ccctgcgtcg ccttcatcac
    10921 ctggccgatc agggagttga aggccttgtc cttgccggcg cggtaatcct cgacctgcct
    10981 cgcgttcttc gccagcacct cgtcgacgat tcgctcgatg gcgccggcat cggagacctg
    11041 cgcgttgtcg gctatcagct tttcaaattc cgccttgcct accgcgacgt tcttgcgacg
    11101 ccaaagctcg gggaagatct tgtctttggc gatcttgttc gaaatggtgc catccgcgat
    11161 gcggccgatc atccaggcca ggacttccgc agacacgaaa gcggcgccga tgtctatgcc
    11221 gtcttcatta agtcggcttg cgagctcgcc gttgatccag ttggccgcga gcttcgggtc
    11281 cgcaccggcc gcgacctgct cgaagtagcg cgcggtctcg cggctcgcgg tgatgaggcg
    11341 cgcgtcgtac tccgataggc cgtgggccga ctgcaggcgc ctggccatcg cctgcggcaa
    11401 ctcgggcagt tgcccgccca gccgctcgat gtcctcggtg ctcaccgcca gcggcaacaa
    11461 gtccggatcg gggaaatagc ggtaatcgtg cgcctcttcc ttggtgcgca tggagcgcgt
    11521 ctcgcccttg tcggggtcgt acaggcgcgt ctcctgcacc accgcgccgc catcctcgag
    11581 cagcgcgatc tgccgccgcg cctcgaactc gatcgcctgc tccatgaagc ggaacgagtt
    11641 caggttcttg atctcgcagc gcgtgccgag cttggcgtcg ccggcgcgcc gcaccgagac
    11701 gttggcgtcg cagcggaacg acccctcctg catgttgccg tcgcacacgc cgatccagcg
    11761 caccagcgca tgcagcgcgc gcgcataggc caccgcctcc ttcgccccgc gcagctcggg
    11821 ctcggacacg atctcgagca gcggcgtgcc ggcgcggttc aggtcgatgc cgctcatgcc
    11881 gtggccgagc gccctgtgcg agtgtggcaa ctcgtgcagc gacttgcccg cgtcctcctc
    11941 gagatgcgcg cgcgtcaggc gcacggtctt ctcgccgccg ggcgcggcga acgtcagtgc
    12001 gcccccggcg acgatcggga tctcgtactg gctgatctgg tagcccttcg gcaggtcggg
    12061 gtagaagtag ttcttgcgcg cgaacaccga gcgcgcattg atgcggccgc cgacggcgag
    12121 cccgaacttc accgccagcg ccaccgcggc ggcgttggcc accggcagca cccccggcaa
    12181 cgcgagatcc accgcgcagg cctgcgagtt gggcggtgcg ccgaacgccg ttgccgcgcc
    12241 ggagaacatc ttgctctgcg tggcgagctg cgcatgcgtc tccaggccga cgacgatctc
    12301 ccaggccatc gccctacctc gaattcaggc gcaggagctg gcagccgcca cggcgatcga
    12361 tcagcaccgg ctccaggtac gcgagcgagc cgcagtgctc ggcgcagtct tcggcgctca
    12421 cgcgcacgag ctcgcccagg tcgcgcagcg tgacgcggca gcgcccggcg acgaagcgca
    12481 gcccgccctc gaccggctgc tgctcggcag tcttcagcgc gcagatgtgg cccgccggcg
    12541 cgacgcgcga gctccacgcc aggcgctgca gccggccctc gtgcacggca agatcgagcg
    12601 tcgagctgta gcgaccctcg cccggctcgc agcgcacctc ggcgctgtac cggttcatgt
    12661 cgggaaactt ctcgctgaac tcgaagcgct cccgctcctg gccgccgggg ccatagcgcg
    12721 tgcccttgaa gacacccccg gtccatgtgc cctgcagcgt ggcggtggcc ggccggccgt
    12781 agaggatggg cgaactgccc tccttgagga acgtgacgcc gagctgcttg ctgcgctcgc
    12841 cctcgaggaa gcgctgcagc agcgtgggca ggatcaggta ctggccggtc aggcgcgccg
    12901 agccgtccgg gcgcggattg tcgtacagcg tcatcaacgc ggcgcgcccg ccgagcatgc
    12961 cgaggagctc gcgcgtttcg cccgcgtcgt cggcccgcgc cgcggtcagc gcgagcatga
    13021 gcagcaccgc gcccagcgtt ctcacagcgg cgcctccttc ggcacgcgca ggtgccagtc
    13081 ggtggcgcgc tggtactggt gcgccgcgcc gagcagcagc gcctcggcaa agtagctgcc
    13141 cgtcagctgc aggccgaccg gcaggccctg gccatcgaag ccgcagggca ccgacagcgc
    13201 cggcaggccg gcgagcggcg ccggaatggt gaagatgtcg ttcaggtaca tctgcacggg
    13261 gtcgtccatc ttggcgccga gggcgaacgc cgtggtcggc gtcgtcggcc ccatgacgag
    13321 gtcgcactgt gagaaagcgg cggcgaagtc cctcgcgatc aagcgccgga ctttctgcgc
    13381 cttgaggtag tacgcgtcgt aatagccgtg cgagagcacg taggtgccca ccagaatgcg
    13441 ccgctgcact tcggcgccga agccttcggc gcgcgtgcgg ctgtacatgt cgacgaggtc
    13501 ggcgaactcc cgagcgcggt ggccgtagcg cacgccgtcg aagcgcgaca ggttcgacga
    13561 cgcttcggcg ggcgcgagca cgtagtacac gggcaccgcc agcgcggcgt tgggcagctc
    13621 gagcggcacg gtgcgcgcgc cctgctgcgt gaaccagtcg agagccgcct gcacgcacgc
    13681 gcgcacaccc ggctccacgc cctcgccgaa gtattcgcgc ggcaggccga tgcgcaggcc
    13741 cttcaggctc aggtcgaggc tgcgcgcgta gtcctccttc gggcgcgcga ggctcgtcga
    13801 gtcgcgcgcg tcgtggcccg ccatgacgtt gagcagcagc gcaagatcgg cggcgctctt
    13861 cgcgatcggc ccgccctggt cgagcgacga ggcgaacgcc accatgccgt agcgcgacac
    13921 caggccgtag gtcggcttca ggccgcacac gccggacagc gcggccggct ggcggatcga
    13981 gccgccggta tcggtgccgg tggccaccgg tgccatgcgc gcggccaccg ccgccgtcga
    14041 gccgcccgag gaaccgccgg gcacgcgcgt gcggtcccag ggattgcgca ccggcccgaa
    14101 gtacgaagtc tcgttcgacg agcccatggc gaactcgtcc atgttggtct tgccgagctg
    14161 caccgcgccg gcggcctcga agcgctcgat gacatgcgcg tcgtacgggc tggtgtagtg
    14221 ctcgagcatc ctcgagccgc aggtggtgcg cagggcgcgt gtgcagaaga tatccttgtg
    14281 tgccaccggg atgccggtga gcggcccggc ctcgccgcgg gcgcggcgcg cgtcggcggc
    14341 gcgcgcctgc tccaggctga cctcctcgtc caccgtgatg aacgcgttca gcggcccgtt
    14401 ctgggcgcgg atgcgctcga ggtacaggcg cgtcaactcg acgctggata cgcgccgcgc
    14461 ggccagcgcc gcctgcagct cgccgaggcc ggagtcgagc acttactcga tcaccttcgg
    14521 taccaggtac agcccgtcct cgaccgcggg ggcggcgacc tggaaccgct cgcgctggtc
    14581 gctctcggtc accgtatcgg cccgcagcgg ttgcgtgacg tccagcgcgt gcgacatggg
    14641 ctcgatgccc tcggtgtcca ctgccttcag ctggtcgagc atggcgagga cccggttcag
    14701 gcgctcgccg accgcggccg attcgtcctc gcgcaaggcg atgcgcgcaa gccgggcgat
    14761 cagccggatc tggtcagggg aaagcgacat tacggcggcg caaaaaaaac gggttcgaag
    14821 gccggtcccg tgcttgcccg gggcctggtt gcgggtatta tatgcccttt agcgacacag
    14881 ccttagcgta caaagttaag gcgcaaccca taaagcggcg gccaccacca acatgttcgg
    14941 atttctgcgc agctacttct ccaacgatct ggcgatcgac ctgggcaccg ccaacacgct
    15001 catctacgtg cgcggcaaag gcatcgtgct cgatgaaccc tcggtggtgg cgatccgaca
    15061 gcaaggcggc cccaacgcga agaagaccat ccaggcggtc ggcaaggaag ccaagcagat
    15121 gctcggcaag acgccgggca acattgtcgc catccggccg atgaaggacg gcgtgatcgc
    15181 cgacttcacc atcaccgagc agatgctcaa gcagttcatc aagaaagtgc acgacaacaa
    15241 gctgttttcg ccgagcccgc gcatcatcat ctgcgtgccg tgcggctcga cgcaggtcga
    15301 gcgccgcgcg atccgcgaga gcgcgatcgg cgccggcgcg ggccaggtgt acctgatcga
    15361 ggagccgatg gcggcggcga tcggcgccga cctgccggtc gcggaagcca ccggctcgat
    15421 ggtggtcgac gtcggcggcg gcaccaccga ggtgggcgtg atctcgcttg gcggcatggt
    15481 gtactcgggg tcggtgcgcg tcggcggcga caagttcgac gaggcgatca tcaactatat
    15541 ccgccgcaac tacggcatgc tgatcggcga gaccaccgcc gagaccatca agaaggaaat
    15601 cggctccgcc ttccccggga gcgaagtgcg cgagatggaa gtcaagggcc gcaacctcgc
    15661 cgagggcatt ccgcgcagct tcaccatcag ctccaacgag atcctcgaag cgctcaccga
    15721 gccgctcaac cagatcgtcg gctcggtgaa gtccgcgctc gagcagaccc cgcccgaact
    15781 gggcgccgac atcgccgaga aaggcatggt gctcaccggc ggcggcgcgc tgcttcggga
    15841 tctggaccgg ctgttcatgg aagagacggg cctgccggtg atcgtcgccg acgacccgct
    15901 cacctgcgtg gtgcgcggct cgggcaaggc gctcgaaaag atggagtact tcggcccgat
    15961 cttcactaac gactgagcga agagggcgcc cgcgcgccgc ggccgccctc ttcttacgcg
    16021 atggagcaca cgcccccgcc ctttttcgta cgtggccccg ctcccctcgt ccggctcgcg
    16081 ttcttcgcct cgctttcgct gacgcttctg gtgctcgacg cgcgcttcgg ctacgccgag
    16141 gccatccgca gcgtgctgac gctcgccgcc tatccgatcc agcaactcgc acgcctgccg
    16201 gtggccgccg tcgaggggct cggcggctac ttcgccagcc aggcgcacct gcgcgaggag
    16261 aacgccgcgc tgcgcgcccg gctgctcgcc gcggcgctcg atgcgcagcg cttcgagtcg
    16321 gcggccgccg aggccgccaa tctgcgccgc ctgatcggag cggccgagcg ccatgaccgc
    16381 aaggccctgc ccgcagagat cctgtactcg gggcgtgacc cgtacgcgcg caaggtgatc
    16441 gtggaccggg gcgcacaaca cggtgcgcgt ccgggcgcgc cggtggtcga tgacatcggc
    16501 gtcatcggcc agctcacgcg cgcgcacgct ttcgtctccg aagtcaccct gctcaccgac
    16561 aagaaccagg cgatcccggt gcaggtgctg agaaacgggc tgcgcgcgat cgccttcggc
    16621 ggcggcgcct cgggtctgct cgagctgcgc tacatggcgg ccaacgcgga ggtggagagc
    16681 ggcgacctgc tcgtcacctc gggcatcgac ggcacgtatc cgccgggctt gccggtggcc
    16741 gaggtggtgc gcgtcgagcg cgatgcttcc tacacgttcg cgcgcgtgct ggcgcagccg
    16801 gtggcgggca tcgagcgcgg ccgctttgcg ctgctgctct cggcgggcgc ccgcccgccc
    16861 gcctatcccc aggacgccga cgagaagaaa gggctgaaga cgcggcgtgc acgcaagccg
    16921 ggggccggga atggctgagc gtccgcagca catcctgatg ccggtacgca ccggcgtcat
    16981 cgtcgcgtcg ttcatcggcg cgctgatcct ggacttcttg ccctggcccg atgtcatgct
    17041 gacgccggac ttcgtcgccc tgaccctcgc cttctggtgc gtgcgccagc cgcggcgcgt
    17101 gggccttgcc accggcttcg cgctcgggct gctggtggac gccggcaacg gcgtgctgct
    17161 cgggcagcac gcgctcgcct actcggctct cgcctttgcc gccattggct tgtcgcgccg
    17221 catcctgtgg ttcgacatgc cggggcagat gcttcacgtg ctgctcctgc tcgaaggggc
    17281 ccaggccgta gcgctcggcg tgcgcctcgc cgcgggcgcc gagttcccgg gcccaacgct
    17341 gttcatcggg ccggcggccg ccacgctgtt atggccggtg gtgagctggc tgctgcggct
    17401 gccgcagcgc cggtcggcgc tgctcgacga gcgccggtcg ctatgaggtt cgccgagctc
    17461 aggaacaccg agagcgagct gcgcgccttc caggtgcgcc tgggcgtcgc gggcctggcc
    17521 gtgctgatcg cattcgcgct gctgctggcg cgcttcgtct ggctgcaggt gctgcagcac
    17581 gacgcctaca gcgccaaggc ggaggaaaac cgcatcgccg tcgtgccgct gccgccaaac
    17641 cgcggcctga tcctcgatcg caacggcgag atcctcgccc gcaactacct cgcgtacacg
    17701 ctggagatct tcccggcgca ggtgcgtgac ctcgaggcca ccatcgccca gctcgccgag
    17761 ttcgtcgaca tccagccgcg cgaccgtgcg cgcttccgca agctcctgca ggagaccaag
    17821 aacgccgaaa gcctgccgat ccgcacgcgg ctctccgacg aagaagtggc gcgcatcgcc
    17881 gtcaatcgct ggcgctttac cggtgtcgac atccgggcgc gcctgtttcg ccgctacccc
    17941 tacggcgagc tcgcttcgca cgtgctgggc tacatcggcc gcatcacgac gagcgacctc
    18001 gagcaactgc agggcatgca ggtcgatgcc aactatcggg gcacggacta catcggcaag
    18061 gcgggcatcg aagcaagcta cgagcaggag ctgcatggca ccaccggttt cgagcgcgtg
    18121 gagatcgacg ccgccggccg cggcatccgt gcgctctcgc gcacgcccgc gcagcccggc
    18181 aacaatgtcg cgctgaccct cgacatccgc ctgcaggaag tggctgagcg caccttcggc
    18241 gagcgccgcg gtgcgctggt cgcgatcgag ccggccaccg gcggcgtgct cgcgttcgtc
    18301 tccagcccgg gctacgaccc gaatctcttt gtcgacggca tcgacccgca gagctgggag
    18361 gcgctgatga gctcgcccga ccggccgctc aacaaccgcg ccatcgccgg cctgtacccg
    18421 cccggctcga ccttcaagcc gtacatggcg cttgcggcgc tcgagctcgg caagcgcacg
    18481 ccgcgcacca ccatttccga cccgggctat ttcgtgttcg gcgaccggcg cttccgcgac
    18541 tccaagcccg gtggtcacgg cacggtggac atgtacaagt cgatcgtcgt gtcctccgac
    18601 gtgtactact acatgctcgc caatgacatg ggcatcgacg ccattgccgg attcatgaag
    18661 cgctttggct tcggcgcgcg caccggcatc gacctcgagg gcgaggccga gggcgtgctg
    18721 ccctcgccgg agtggaagca cgcgcgcttc tcgcggcccg agcagcagcg ctggtatgcg
    18781 ggcgagacca tctccatcgg catcggccag ggctacaacg cctatacgcc ggtgcagctg
    18841 gcgcaggcgc tggcgacgct cgccaacaac ggcgccatgt accggccgcg gctcgtgtcg
    18901 cacatcgaca acccgcgcac cggcgagcgc cagtacctgg agccgcagct ggtgcgccag
    18961 gtgcagctgc agccggagca catcgcgttc atcaaggagg cgatggccgg cgtgaacctg
    19021 gagggcaccg ggcgctgggc gttcgccggc gcgcagtaca cgagcggcgg caagaccggc
    19081 accgcgcagg tggtgggcat gaaggcgggg gagaagtacg aggaggacaa ggtcgccgag
    19141 cgcctgcgcg accactcgct ctacatcgcg ttcgcgccgc tcgagggccc caagatcgcg
    19201 ctcgccgtgc tggtggaaaa cggcggcttc ggcgcgcgcg ccgcggcgcc gatcgcacgc
    19261 accgtgctcg actactacct gctcggcaag ctgccgaaag agccgcggcg cgcggtgccc
    19321 gaggccgaag tgaagagcga cgcggagagc gactgatgga gatcgccggc tacccgctgc
    19381 ggcgcctgcg cgcgcgcgtc gtcgagggca ttgacgcgcc gctgctcgcg atcgcggtgg
    19441 cgctcagtct ggtcgggctc ggcgtgctgt actcggcgag ctacgagaac gccacgcgcg
    19501 tggcgagtca actggcgaat ctcgcggtgg cgctggccct gatgtgggcg gtggcgcagg
    19561 tggcgccgca gacgctggcg cggctcgcgc tgccggccta ctgcgccggc gtcgccctgc
    19621 tcgtcgccgt ggcgctgggc ggcgaggtgg tgaacggcgc gcgccgctgg ctgaacctgg
    19681 gcgtgatgcg cttccagccc tcggagatga tgaagatcgc gttgccgatg atgctggcgt
    19741 ggtacttcca ccgcaacgag ggcgcgatcc ggctgcgcga ttttgcggtc gccggcgtgc
    19801 tgctcgcgct gccggtggcg ctgatcgcgc gccagcccga cctcggcacg gcactgctgg
    19861 tgaccgccgc gggtttctac gtcatcttcc tcgccggcgt gagctggcgg ctgctcgccg
    19921 gcctcgccgt actgtcgatc gccagcctgt ttccgctctg gggcctgctg cacgactacc
    19981 agcgccgccg gatcctcacg ctgctcgacc cgttgcagga tccgctcggc gcgggctacc
    20041 acaccatcca atcgacgatc gcggtgggct cgggcggcat tgccggcaag ggctggctga
    20101 acggctcgca gacgcacctc gagttcattc cggagcgcca caccgatttc atcttcgccg
    20161 tgttctccga ggagttcggg ctggtgggca acgtgctgct gctcgcgctg tacgcgctgc
    20221 tcgtcgcgcg cgggctcgcc atcgccgcgc aggccgcgac gctgttcgcg cggctgctcg
    20281 cgggcgcggt gacgctcatc tttttcacct acgcattcgt caacatgggc atggtggccg
    20341 gcatcctgcc ggtggtcggc gtgccgctgc ccttcgtttc ctacggcggc acgtcgctcg
    20401 cgacgctgtt cgtcggcgtg ggccttctga tgggcatcca gcgccaccgc aagctcgtcc
    20461 aaacctgagt tttgcgcagc agcagcattc tgaaaccggc atccgcatca tgaaacgatg
    20521 ccggcctaag tctctgtaaa tccttgtttt gggaatacta agacggcggc agatgaattg
    20581 ttgctgcgcg gtatttttct gggctatggt cgaagggagg ctttctccca acctaacctg
    20641 aggattccca tgcagaaacg tgacccggaa atcgccgcac tgaacaagga ctggaccgag
    20701 aacccgcgct ggcagggcat cgagcgcggc tatacggccg aggacgtcgt gcggctgcgc
    20761 ggcagcgtga agatcgagca cacgctggcg cgactgggcg cggagaagct gtggaagctg
    20821 tgccgggagc ggccgttcgt gaactcgctc ggcgcgctca ccggcaacca ggccatgcag
    20881 caggtgaagg cgggcgtgcc cgcgatctac ctctcgggct ggcaggtggc cgcggacgcg
    20941 aacgactcgc tttccatgta cccagaccag tcgctctacg cgacgacttc ggtgccctct
    21001 gtcgtcaggc gcatcaacaa cgcgctcatg cgccaggacc agatccagac catggagggc
    21061 aagggcgaga tcgactggtt cgcaccgatc gtcgccgacg ccgagtccgg cttcggcggc
    21121 gtgctgaacg ccttcgagct gatgaaggcg atgatcgagg cgggcgccgc gggcgtgcac
    21181 ttcgaggacc agctcgcctc ggtgaaaaag tgcggccaca tgggcggcaa ggtgctggtg
    21241 ccgacgcagg aggcggtgca gaagctgatc gcggcgcgcc tcgccgccga cgtgatgggc
    21301 gtacccacgg tgctgctggc gcgcaccgat gcggaggccg ccgacatcgt gacctccgac
    21361 gtggacgaga acgacaagcc gttcatcacc ggcgagcgca cccccgaggg tttctacaag
    21421 acgaaaaagg gcttcgagca ggcgctctcg cgcggtctcg cctacgccga atacgccgac
    21481 atggtgtggt gcgagaccgg caccccggac ctggcgttcg cgcggaagtt cgccgagggc
    21541 attcgccgca agtacccggg caagatgctc gcctacaact gctcgccgtc gttcaactgg
    21601 aagaagaacc tggacgacgc cacgatcgcc aagttccagc gcgaactcgg cgccatgggc
    21661 tacaagttcc agttcatcac gctggcgggc ttccattcgc tgaactactc gatgttcgag
    21721 ctcgcctacg gctatgcgcg cgacaacatg agcgccttcg tggcgctgca gcagaaggag
    21781 ttcgccgctg ccgcgaacgg cttcaccgcg gtcaagcacc aacgcgaagt cggcaccggc
    21841 tacttcgacg agatcacgca ggtggtctca ggcggcaagg cctcgaccac tgcgctgcac
    21901 ggctcgaccg aggacgcgca gttcttcgag gaaagggcga agaaggtcgc ctgagttgac
    21961 ggcgccggcg aaggtcctgc ccatccacgc ggcgcgcggc gagcgcgccg gcgaggtggc
    22021 gcgcaccatt ctcgccggct tcgacaagca ttaccgcctg ttccgcgagg cctcggcgcg
    22081 cgcgaagcac ctgttcgagc gcgccgactg ggcggcgatg ggcgagctcg cgcgcgcgcg
    22141 catcctgatg tacgaccgcc gcgtgcagga ggcggtcgag gaggtgcggt cgcactttcc
    22201 ggaagccgag accgacgagt cgctgtggcc ggcgatcaag ctcgcctaca ttccgctcct
    22261 gcacgagcac cggcagcccg aatgcgccga gaccttctac aactccgttg cctgccaggt
    22321 gctgcaccgg cgctactacc gcaatgagtt catcttctgg cggccggcgg tggccaccga
    22381 gcacctcgag ggcgaagagc ttgcctatcg cagctactat ccgcgcagga agaccctgcg
    22441 gcggaccctg gcggcgatcg tgcgcggctt cgacctcgcc aacccctggg aagacctgca
    22501 ccgcgacctg cgcagcgtcc tgcgcgcgtt gcgcgcgcgc tttccgcgcc ctgcgcgcgc
    22561 gcgccccaac ctgcagatcc aggtcctggg gtcgctcttc atccgcaaca aggccgccta
    22621 cgcggtcggc aagatcgtga acggcagcga ggaagtgccg ttcgcggtgc cgatcctgca
    22681 gaacgagcgc ggcgagctct acctcgatgc gctgctgctg gaggccgacc agctgctcgt
    22741 cctgttctcc tttgcgcgcg cctacttctt cgtcgatatg gaagtgccgg cggcctacgt
    22801 gtcgttcctg cgccatctga tgccgaggaa gccgcgcgcc gagatgtaca tggcggtggg
    22861 cctcgccaag cagggcaaga ccatgttcta ccgggacttg ttttatcacc tgaagcattc
    22921 gtcggacctg tttacggtcg cgcccggcat caagggcatg gtgatgctcg tgttcaccct
    22981 gccgtcgttt ccctacgtct tcaaggtgat ccgcgaccgc ttcgccccgc ccaaggagat
    23041 ggaccggcag acggtgctcg acaagtacct gctggtgaag atgcacgacc gcgtcgggcg
    23101 catggccgac acgctggagt actcgctggt ggcgctgccg cgcgagcgct tcgcgcccga
    23161 gctcctcgaa gagctgaagg ccgaagccgc ctcgaacatc gaattcgagg acgacaaggt
    23221 ggtcatcaag cacatgtaca tcgagcggcg catgcagccg ctcaacctgt acctcgagga
    23281 ggcgatcggc gacggcgcca tcgagcgcgt gcgccaggcg ctgcacgagt acggcgacgc
    23341 catcaaggag ctggccggcg ccggcatctt tccgggcgac atgctgctga agaacttcgg
    23401 cgtcacgcgc cacgagcgcg tcgtgttcta cgactacgac gagatcgcct acatgaccga
    23461 gtgcaacttc cggaggatcc cggcgccgcg ctcgttcgac gacgagatgg ccgcggagcc
    23521 gtactactcg atcggcccgc acgacgtgtt tcccgagcag ttcgagaagt tcctcgtcac
    23581 cggcgcggcc tcgcgcgcgc tgttcctcga gcgccacgcc gacctgatgg acccgcagtt
    23641 ctgggccgac aagcaggcgc gcctgcgcgc cggggtgcag gaggacgtct ttccctaccc
    23701 gcaggagatc cgcttccggc gctagccggc aggcccggct gtgttagcgt agtcgcccca
    23761 atggcagtgt cggtggcgat gtatgccaat tcctacggcc gcatcaagga tcgtcttgac
    23821 gcgctgcggc tcgacgtcga catcgtcacg ttcgggctcg acggcaagtt cgagatcgac
    23881 ggcgcccggg tggatcctgc ggaggtcgat ctcgactatc tctggctcag tccggagctg
    23941 agcctcgagg gcggcctgcg gaccgcattc gaggtggcgc tccggtgccg atcgatcaag
    24001 gtgctgcaga ccttcaacgc cggcctggac gctccggcgt acaagcagat cgcggccaag
    24061 ggcgtgcgca tttgcaacag ctccgcccag gcgatcggga ttgccgagta tgtgatggcg
    24121 caggtgctcg cgctgttcca gcccctggcc gagcagcgcg agctgcaggc gcgcaggacc
    24181 tggcagcgca cgccgttccg cgagatcgcc ggcacgaact ggctgatcct cggctttggc
    24241 ccgatcggcc gcgcgatcgc gcaacgcgcc aagggctttg ccgcccacgt ccacgtcgtt
    24301 cggcgtacgc cggagaccgg cgcgacggtc gatcgcgcgg gcaccatcgc cgaccttcct
    24361 actttcctgc cggccgccga cgttgtcgtc ctcgcctgcc cgctgaacga tgccacccgg
    24421 ggcctggcga acaaagcttt tttcgatgcg atcaagccgg gggccgtgct ggtcaacatc
    24481 gcccgcggcg gattgatcga ggatgccgcc ttgcgccagg cgctcgacga gggccggctg
    24541 gccgccgcgg tgctcgatgt ctttcggacc gagccgctgc cggccgacga tccgctttgg
    24601 acgcacccga agatccgact gacctcgcac acatcgtttt cgggcgacgg cgtgcgccgg
    24661 cgctgggagc agttgtttct cgataatctg ccgcggttcg tcaggggcga agcgctcctg
    24721 aacgaggtcg atcccaagga tctcgcctag agcgcgctcc acgccgccgt gcgcaggtgc
    24781 tcgagcgcga tgagcgccac ctgcagcagc accagcagca ccagcggcga caggtcgacg
    24841 ctgccgagca gcggcaccac gcgccggatc ggccgcagga acagccgtgt catggcgtcg
    24901 aacaggtaca tcaccggcga gtgcggattg acccaggaca gcaccgcctg cacgaccacc
    24961 gcaaacacca ggatgtacag gctgtagcgc acgaggtcga acgccgaagc gacgaacacc
    25021 agtgtcgagc ccgagccgcc cgccatggcc acgagcagcg tgagcgccag cgcctgcagc
    25081 agccaggcgg cgatcagtgt cgcgagatcg agccccgcga gcggcg
//
LOCUS       DBNHKE010000079        10903 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_648835, whole genome shotgun sequence.
ACCESSION   DBNHKE010000079 DBNHKE010000000
VERSION     DBNHKE010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_648835"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16O9_05045"
     CDS             complement(<1..200)
                     /locus_tag="AC16O9_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516014.1"
                     /translation="MNRCLLALLIAASPAQAADNPVERGLKAAGKAIERGAKAAGRGV
                     ERGGKAVIRTLEKGHEKVDQKV"
     gene            complement(210..1541)
                     /locus_tag="AC16O9_05050"
     CDS             complement(210..1541)
                     /locus_tag="AC16O9_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6516015.1"
                     /translation="MPPSQRNARGGAVPAFEPQVQLGKYPILREIGRGATSHVYLARD
                     PFADREVAIKVFLFDQDADPQTERMMHKSFLAEASLAGKLNHPHIVDILDAVAEPDHS
                     YLVMEYVAGTALDAHADVATLLPLDKVVEIIFKCIRALEYAYQHGVIHRDIKPANILL
                     SRAGETKVSDFGASFQQRLGQETTQISAIGSPAYMSPEQIRMESLTHQTDIYSLGVTM
                     YRLLTGRLPYQASTQAALTYAILNTPPVRPSMLRPELPPLLDKIVMSAIHKEPGGRYR
                     SWLDFGKELSQAFNTLQLVGDALSDSAKFNELRQMAFFEGFGDVALWEVVRIGAWKRI
                     PSGTVIIREGDHGDSFYLLIAGEVSVTLSGKHLNTVKPGGCFGEILYFTEHVQRRTTT
                     ISAVGDITVIEIKAEALRAATDACQVSLNKAFMRVLIERLMQANVQLAARG"
     gene            complement(1522..6495)
                     /locus_tag="AC16O9_05055"
     CDS             complement(1522..6495)
                     /locus_tag="AC16O9_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6516016.1"
                     /translation="MGSASTTILSWITTEVDQALSLVHEHIARFMADPESTAAMGACQ
                     EHLHQVSGALRMVGLAGATRFCEAIEGGFAGVNGPRMNVAAVGAIDRAVLALKEYVDG
                     LERGQANVPLRLYPIYRELSELQGAQDASEKDLFFPDLALQAPTHPDARTLGPGELKP
                     FLQAQRACFQRGLLALLRGQGGKAQMQQALDALHQIAVQLPEPRALWWIAPALVDALG
                     EPADAEWLARAKVLCNKIDFQMRDLDSGAPALSETLLRDVLYAVAKSKPATQRIREVK
                     QLYLLDSLFPEAQSGEVVKFDMDWLQPALADVRSRLEALKSMWLQYISGEPKSAARFR
                     ELVGAFKAKAGELGNPHLVRLLDAIAVVATRLPDPYPAKDQLLMIEMASAFLLAESVV
                     ENFSDPAADIGEQIGIMGGWLLDAAKGRSSGEPPPGLRADLSEHIGAMQLRAHVAQEI
                     AANLQHVEQVLDGFARDPGKRAALRGLAPYLRQIHGALNVLGFSRAAEILPVCEELIA
                     VCAEPDHDDGDGHDMDWIAEGLSSVGFFLDPCLQGREPAEQAVELFFRRYDKAHAPAE
                     TRAFDSTVVLPGVMQREVPQTSSAPSPARLQVNDELLAVYLEEASEVLAATNAALPVC
                     RAQPLDKDALTTIRRGFHTLKGSGRMVGLMDLGEVAWEIEQVMNKWLEQQRPATPALL
                     ELIDAAGARFADWIAKLSAGELLGEIDGSDIVERARNLKNEAPQPLPAPVAAPAAEPP
                     AAPPDAAPPELAQEPEPEPEPQPEPAPAPEPEPDEVTIGGLRLPRSLFEIYMREAAQH
                     AATLEAQCARWRKAFGSEAAPEFVRAAHTLASSSRTAGFEQVATLAEALEQWMPHARL
                     TAAADAGMVQVAVANLRAMSEALARREPPQAAPALVQGLHELIARLRAPAPPPAPRAQ
                     PAEQGGGREKRVMQDDIDPQLLPIFLEEAQQLLPQIGSDLRDWKASPEDMRVQQSLQR
                     ALHTLKGSARMAGAIRLGELTHIMESRIEAAIEAQLLTPALFAELEEKMDRLSLDLER
                     MAAGPEVPGAAAPAAIEPPLPSPASMLRINADTLDQLINESGEVSIARSRIEGELRTV
                     KQSIGDLTDSIARLRGQLREVEIQADSQMQSRLSVLEERDRDFDPLEFDRYTRLQELT
                     RLMAESLHDATAIQQTLSKSLGETDAALMHQSRVSRDVQQELMRMRAVPFSILDERLY
                     RIVRQTTRELDKKAELEIQGSQVELDRSVLERIGAPLEHMLRNALAHGLESPAERAAA
                     GKPESGRISLTLRQEANEIVLVVSDDGAGLDLERLRHKAEARSLLPPGAAPGVAELTQ
                     LIFVSGLSTADEVTELAGRGIGMDVVKNEVGAIGGRVEVASERGRGTTFTVYLPLTLA
                     VTQAVLVRAGNDILAVPSAMVVQVLRLRAEAFAAHLDTGEIEFQGRAYPLHGLAQVLG
                     GAAPAAAPGYHSVLLLRSGMQRVALHVNELVGNQEIVVKNIGPQLARVPGVSGATVLA
                     DGSIVLIVNPVMLALRARLAPERAAAPAPAQPAALAMVMVVDDSLTVRKVTGRLLERE
                     GYEVLTAKDGVDALEQMKDVLPAVMLVDIEMPRMDGFDLTRQVRADPRTRHIPIIVIS
                     SRTAEKHRDQALQLGVNAFLGKPYPEAELLKRIGEFVAARNDAAIAA"
     gene            complement(6504..8624)
                     /locus_tag="AC16O9_05060"
     CDS             complement(6504..8624)
                     /locus_tag="AC16O9_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR6516017.1"
                     /translation="MAINIGAFKLPFLGRGSDAGAPAGAKRAEAGASALPVIGQLPIA
                     RQLQLLIGALVFLLAVIAALVVYDTRQGTFGTLYIASVGKLQMLSQRLAKAAQQASQG
                     NAEAFKQLRSSRDEFAAIVKLLAFGGTTGGVDLPPTPRDVQPSLNALDRSWGKTERNA
                     ALVIDEERNLVALGSAVRAINANNPVLQELTDEMAALSVQKGGSARQNAITAQLTMLT
                     QRMAKNANIMLAEDIVDPEVAFLLGKDTNTYRDTVQGLLQGSDALRIARVADAEMRGK
                     LGELQSAFADYQRAVSEILGNQARLVGAKRAAFDIFNDSEALLRSAEGLSAGFETDLA
                     KRRFNFIALGVLALLALGVLALIGVVYIADSRRRAEDSERQNKANQQAILRLLDEIGS
                     LADGDLTSRAQVTEEITGAIADSINYAIDELRRLVAGINTAAQQVTTATAEAQSTASQ
                     LLQAAQKQAVEIEGTGQSVTQMARSMSEVSRSAGDSAKVAESSLHAAEKGAQAVQNAI
                     RGMNDIREQIQETSKRIKRLGESSQEIGEIVQLISDITEQTNVLALNAAIQAASAGEA
                     GRGFTVVAEEVQRLAERSAEATKHIGAIVKSIQRDTQDAVEAMERSTRGVVEGTKTAD
                     EADRALREIEQVSNQLAELIGAISNATDQQAASARRVAVAMQEILAITQLTTDGTRTT
                     AASAERLTELANGLRASVAGFKLA"
     gene            complement(8635..9771)
                     /locus_tag="AC16O9_05065"
     CDS             complement(8635..9771)
                     /locus_tag="AC16O9_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516018.1"
                     /translation="MKRIAAMAAGGAIGALLAFPAPAETGVTADRVLFGQAAVFSGPA
                     AQLGIQMRNGIKAYFDYVNARGGVHGRKIDLITEDDRYETAVAPVASKKLIDEHKVFA
                     LIGYVGTPTGVAHLPVVTQAKVPLVGMFTGAEVLRVPFNRYVFHVRASYYDETDKIVE
                     QVVSIGGKKIAVFYQNDAYGQAGLKGVELATQKRGMKITALGTVERNTVRVEDAVKQI
                     HAARPDAVVMISAYTSCAEFIRQMQSAGSNATFYNVSFVGSKALSDALGKVGVGVAIS
                     QVVPFPWGRAVPVVKEYQQLSEKSGHTDYNFSAMEGFLSAKVAVEGLRRAGRNLTRES
                     YIAALEKMNDADLGGFYVSYSPTNRAGSKFVDLTIIGRGGKFLR"
     gene            complement(9798..10307)
                     /locus_tag="AC16O9_05070"
     CDS             complement(9798..10307)
                     /locus_tag="AC16O9_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6516019.1"
                     /translation="MAARTSLRDYQRDLVARLQSAGAGRAASKLGLQVGGERWLVDLT
                     DAGEVIPVPGITPVPLTRPWFRGVASIRGNLFSVVDIGAFLGGGAAMPGEQARLLLLN
                     ERFRMGSALLVDRSLGLRNPEQLQERAATPQRAPWIRAEYDDPQGAHWRELDVAQLVQ
                     DPEFLSVGA"
     gene            complement(10307..10672)
                     /locus_tag="AC16O9_05075"
     CDS             complement(10307..10672)
                     /locus_tag="AC16O9_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6516020.1"
                     /translation="MPIKNILVVDDSPTDRQYLTDLLAKSGYQVTTADSAERALEAVK
                     QQRPDLVLMDVVMPGQNGFQATRTLSKDSATKDIPVIICTTKGQETDRVWGMRQGARD
                     YVVKPVNQAELLAKISALG"
     gene            complement(10697..>10903)
                     /locus_tag="AC16O9_05080"
     CDS             complement(10697..>10903)
                     /locus_tag="AC16O9_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516021.1"
                     /translation="DGYQTCALIKQNPKLKATPVIMLSSKDGVFDRARGRLAGSDRYL
                     TKPFTKEALIDTVNQYLKPATPSS"
BASE COUNT         1500 a         3849 c         3686 g         1868 t
ORIGIN      
        1 ctgaccttct ggtcgacctt ctcgtgccct ttctcgaggg tacggatcac cgccttgccg
       61 ccacgctcga cgccgcggcc cgcggccttg gcgccccgct cgatcgcctt gccggccgcc
      121 ttcaggccgc gctccacggg gttgtcggcg gcctgcgcgg gcgaggccgc gatcaggagc
      181 gcgagcaggc atcggttcat cgggccctcc tagccgcgcg cggcgagctg cacgttggcc
      241 tgcatcagcc gctcgatcag cacgcgcatg aacgccttgt tgaggctcac ctggcaggca
      301 tcggtggcgg cgcgcagcgc ctcggccttg atctcgatca ccgtgatgtc gcccacggcg
      361 ctgatggtgg tggtgcgccg ctgcacgtgc tcggtgaagt acaggatctc gccgaagcag
      421 ccacccggct tgacggtgtt gaggtgcttg cccgagagcg tgacgctcac ctcgcccgcg
      481 atcagcagat agaagctgtc gccgtggtcg ccctcgcgga tgatcaccgt gccggacggg
      541 atccgcttcc aggcgccgat gcgcaccacc tcccacagcg ccacgtcgcc gaagccctcg
      601 aagaacgcca tctgccgcag ctcgttgaat ttcgccgagt cggatagcgc gtcacccacc
      661 agctggagcg tgttgaaagc ctggctcagc tccttgccga agtcgagcca ggatcggtag
      721 cgcccgccgg gctccttgtg gatcgcgctc atcacgatct tgtcgagcag cggcggcagc
      781 tccggccgca gcatgctcgg gcgcaccggc ggcgtattaa ggatggcgta ggtgagcgcg
      841 gcctgcgtcg atgcctggta cggcaagcgc ccggtcagca gccggtacat ggtgacgccg
      901 agcgagtaga tgtcggtctg gtgcgtgagc gactccatgc ggatctgctc gggcgacatg
      961 taggcgggcg agccgatggc cgagatctgt gtggtttcct gccccaggcg ttgctggaac
     1021 gaggcgccga agtcgctcac cttggtctcc ccggcgcgcg acagcaggat gttggcgggc
     1081 ttgatgtcgc ggtggatgac gccgtgctgg taggcgtact ccagcgcgcg gatgcacttg
     1141 aagatgatct cgaccacctt gtcgagcggc agcagcgtcg ccacgtcggc gtgcgcgtcg
     1201 agcgcagttc cggccacgta ctccatcacg aggtagctgt gatcgggctc ggccaccgcg
     1261 tcgaggatgt cgacgatgtg cgggtggttg agcttcccgg cgagcgaggc ctcggcgagg
     1321 aacgacttgt gcatcatgcg ctcggtctgc gggtcggcgt cctggtcgaa caggaacacc
     1381 ttgatcgcca cctcgcggtc ggcgaacgga tcgcgcgcca ggtacacgtg gctggtggcg
     1441 ccgcggccga tttcgcgcag tatcggatac ttgccgagct ggacctgcgg ctcgaacgcc
     1501 ggtacggcgc cgccgcgcgc gttacgctgc gatggcggca tcgttacgcg cggcgacgaa
     1561 ctcgccgatc cgcttcagca gctcggcctc cgggtacggc ttgccgagga acgcgttcac
     1621 cccgagctgc agcgcctggt cgcggtgctt ctcggcggta cgcgaggaga tgacgatgat
     1681 cggaatgtgg cgcgtgcgcg ggtcggcacg cacctgccgc gtcaggtcga agccgtccat
     1741 gcgcggcatc tcgatgtcga ccagcatgac cgcgggcagc acgtctttca tctgctccaa
     1801 tgcgtcgacg ccgtccttgg ccgtgagcac ctcgtagccc tcccgctcga gcagtcgccc
     1861 cgtgactttg cgcaccgtga gcgaatcgtc caccaccatc accatggcca gcgccgccgg
     1921 ctgcgccggt gccggcgcgg cagcgcgctc gggggcgagg cgcgcgcgca gcgccagcat
     1981 aaccgggttg acgatcagca cgatactgcc gtcggcgagc acggtcgcgc ccgagacgcc
     2041 gggcacgcgt gccagctgcg ggccgatgtt cttgacgacg atttcctggt tgccgacaag
     2101 ctcgttgacg tgcagggcga cgcgctgcat gccgctccgg agcagcagca ccgagtggta
     2161 gcccggcgcg gccgccggcg ctgcgccgcc cagcacctgc gccagcccgt gcaacgggta
     2221 ggcgcggccc tggaattcga tctcgcccgt gtcgagatgc gcggcgaacg cctcggcgcg
     2281 cagccgcagt acctggacca ccatcgccga gggcaccgcc aggatgtcgt tgccggcgcg
     2341 caccagcacc gcctgcgtca ccgccaaggt gagcggcagg tagacggtga acgtcgtgcc
     2401 ccggccgcgc tcgctcgcca cctcgacgcg cccgccgatc gcgccgacct cgttcttcac
     2461 cacgtccatg ccgatgccgc ggcccgccag ctcggtgacc tcgtcggcgg tggacagacc
     2521 ggagacgaag atcagctgcg tcagctcggc gacgccgggc gcggcaccgg gcggcagcag
     2581 gctgcgcgcc tcggccttgt gccgcagccg ctccaggtcg aggccggcac cgtcgtcgct
     2641 gacgaccagc acgatctcgt ttgcttcctg gcgcagcgtc agcgagatgc gcccgctttc
     2701 aggcttgccc gccgcggcgc gctccgccgg tgactccagg ccgtgcgcca acgcgttgcg
     2761 cagcatgtgc tcgagcggcg cgccgatgcg ctccagcacg ctgcggtcga gctccacctg
     2821 gctgccctgg atctcgagct cggccttctt gtccagctcg cgcgtggtct ggcgcacgat
     2881 ccgatacagg cgctcatcaa ggatcgaaaa cggcaccgcg cgcatgcgca tcagctcctg
     2941 ctgcacgtcg cggctcacgc gcgattggtg catgagggcg gcgtcggtct cgccgaggct
     3001 cttcgacagc gtctgctgga tcgcggtggc gtcgtgcagg ctctccgcca tcagtcgcgt
     3061 gagctcctgc agccgggtgt agcggtcgaa ctcgagcggg tcgaagtcgc ggtcgcgctc
     3121 ctcgagcacc gacaggcgcg actgcatctg gctatcggcc tggatctcga cttcgcgcag
     3181 ctggccgcgc agccgcgcga tgctgtccgt aagatcgccg atcgactgct tgaccgtgcg
     3241 cagctcgccc tcgatgcgcg agcgcgcgat gctcacctcg ccggactcgt tgatcagctg
     3301 gtcgagcgta tcggcattga tgcgcagcat cgaggccggg ctgggcagcg gcggctcgat
     3361 cgccgccggc gccgctgcgc ccggaacctc cggccccgcg gccatgcgct cgaggtcgag
     3421 gctcagccga tccatctttt cttccagctc ggcgaacagc gccggcgtaa gcagctgcgc
     3481 ttcgatcgcc gcctcgatgc ggctttccat gatgtgcgtc agctcgccga gccggatcgc
     3541 gcccgccatg cgcgcgctgc ccttcagggt atgcagcgcg cgctgcagcg actgctgcac
     3601 gcgcatatcc tcggggctcg ccttccagtc gcgcaggtcg ctgccgatct gcggcagcag
     3661 ttgctgcgcc tcctcgagaa agatcggcag cagctgcggg tcgatatcgt cctgcatgac
     3721 gcgcttttcg cggccgccgc cctgctccgc cggctgcgcc cggggcgccg gcgggggcgc
     3781 cggtgcgcgc agccgcgcga tcagctcgtg caggccctgg accaaagcgg gcgccgcttg
     3841 cggcggctcg cggcgtgcca gcgcctccga catcgcgcgc aggttcgcca ccgcgacctg
     3901 caccatgccg gcatcggcgg cggccgtcag gcgcgcgtgc ggcatccact gctcgagcgc
     3961 ctcggcgagc gtggcgacct gctcgaagcc cgcggtgcgc gagctgctcg ccaaggtatg
     4021 cgcggcacgc acgaattcgg gcgcggcttc gctgccgaaa gccttgcgcc aacgcgcgca
     4081 ctgcgcctcc agcgtggccg catgctgcgc ggcctcgcgc atgtagatct cgaacaggct
     4141 gcgcggcagg cgcaggccgc cgattgtcac ctcatcgggc tcgggttcgg gcgcaggtgc
     4201 cggctccggt tgcggctccg gctcgggttc gggctcctgt gcgagctcag ggggcgcggc
     4261 gtcggggggt gccgcgggcg gctcagccgc aggcgcggcc acaggcgcgg gcagcggctg
     4321 cggcgcttcg ttcttcaagt ttctggcgcg ctcgacgatg tcgctgccgt cgatctcgcc
     4381 cagcagctcg cctgcgctca gcttggcgat ccaatcggcg aagcgcgcgc ccgcggcgtc
     4441 gatcagttcg agcaacgccg gtgtcgcggg acgctgttgc tcgagccact tgttcatgac
     4501 ctgctcgatt tcccaggcca cttcgccgag gtccatcaaa ccgaccatgc ggccgctgcc
     4561 cttgagcgtg tggaagccgc gccggatcgt cgtcagcgcg tccttgtcca gcggctgcgc
     4621 gcgacagacc ggcagcgcgg cgttggtggc cgcgagcacc tcgctcgcct cttccaggta
     4681 gaccgccagc agctcgtcat tgacctgcag ccgggcaggc gacggcgctg aggatgtctg
     4741 cggtacttcg cgctgcatta ccccgggcag cacgaccgtg gaatcaaagg cgcgcgtctc
     4801 ggctggcgcg tgcgccttgt cgtagcggcg gaagaacagc tccacggcct gctctgcagg
     4861 ctctcgcccc tgcaggcagg gatcgaggaa gaaaccgacg ctcgacaggc cctcggcgat
     4921 ccagtccatg tcgtgcccgt cgccgtcgtc gtggtccggc tcggcgcaaa cggcgatcag
     4981 ctcctcgcac accggcagga tttccgcggc gcgcgaaaag ccaaggacgt tgagcgcgcc
     5041 gtggatctgg cgcaggtagg gcgccaggcc gcgcagggcg gcgcgcttgc ccgggtcgcg
     5101 cgcaaatccg tcgagtacct gctcgacgtg ctgcaggttg gccgcgatct cctgcgccac
     5161 atgggcgcgc agctgcatgg cgccgatgtg ctcgctaagg tcggcacgca gcccgggcgg
     5221 cggctcgccg ctcgagcggc ccttggccgc gtccagcagc cagccgccca tgatgccaat
     5281 ctgctcgccg atgtcggccg ccgggtcgct gaagttctcg accacgcttt cggcgagcag
     5341 gaacgccgag gccatctcga tcatcagcag ctggtccttc gcggggtagg gatcaggcag
     5401 ccgcgtcgcc acgaccgcga tggcgtcgag cagccgcacc aggtgcggat tgccgagctc
     5461 gccggccttt gccttgaatg cgccgaccag ctcgcggaag cgcgccgcgc tcttcggctc
     5521 ccccgagatg tactgcagcc acatgctctt gagcgcctcg agccgcgagc gcacgtcggc
     5581 gagtgccggc tgcagccagt ccatgtcgaa cttgacgact tcgccgctct gcgcctcggg
     5641 aaacaggctg tcgagcaggt acagctgctt cacctcgcgg atgcgctggg tcgccggctt
     5701 gctcttcgcc accgcgtaga gcacgtcgcg cagcagcgtt tcgctcagcg ccggcgcacc
     5761 cgagtccagg tcgcgcatct gaaagtcgat cttgttgcac agcaccttgg cgcgcgccag
     5821 ccactcggcg tcggcgggct cgcccagggc atccaccagc gcgggcgcga tccaccacag
     5881 ggcgcgcggc tcgggcagtt gcaccgcgat ctggtgcagc gcgtcgagcg cctgctgcat
     5941 ctgcgccttc ccgccctggc cacgcagcag cgcgagcagc ccgcgctgga agcacgcgcg
     6001 ctgcgcttgc agaaacggct tcagctcccc ggggcccagc gtgcgcgcat cgggatgcgt
     6061 cggcgcctgc aacgcgaggt cggggaagaa caggtctttc tcggatgcgt cttgcgcacc
     6121 ctgcagctcg ctcagctcgc ggtagatcgg gtacaggcgc agcggcacgt tggcctggcc
     6181 gcgctcgagc ccgtcgacat actccttcag cgccagcacg gcccggtcga tcgcgccgac
     6241 cgcggccacg ttcatgcgcg ggccgtttac ccccgcgaaa ccgccttcga tggcctcgca
     6301 aaagcgcgtt gcgccggcca gccctaccat gcgcagcgcg ccactcacct ggtgcaggtg
     6361 ctcctggcag gcccccatcg cggcggtgct ctccggatcg gccatgaagc gcgcgatgtg
     6421 ctcgtgcacg agcgacagcg cctggtccac ctcggtggtg atccaggaaa ggatggtagt
     6481 ggaggcgctg cccatggccc tcgtcaggcg agcttgaagc cggcgaccga ggccctgagc
     6541 ccgttcgcca gctcggtgag gcgctcggcc gaggccgccg tggtacgcgt gccgtcggtg
     6601 gtcagctgcg tgatggcgag gatctcctgc attgccaccg ccacgcgccg cgccgaggca
     6661 gcctgctgat cggtggcgtt ggagatcgcg ccgatcagct cggcgagctg gttcgacacc
     6721 tgctcgatct cgcgtagcgc gcggtcggct tcgtcggcgg tcttggtccc ctccaccacg
     6781 ccgcgcgtgc tgcgctccat ggcctcgacc gcgtcctggg tgtcgcgctg aatcgacttc
     6841 acgatggccc cgatgtgctt ggtagcctcg gcggaacgct cggcgagccg ctgcacctct
     6901 tccgcgacca ccgtgaagcc gcgtcctgct tcgccggccg aggccgcctg gatggcggcg
     6961 ttcagcgcca gcacgttggt ctgctcggtg atgtccgaga tcagctgcac gatctctccg
     7021 atctcctgcg aggattcgcc cagccgcttg atgcgcttgg aggtttcctg gatctgctca
     7081 cggatgtcgt tcatgccgcg gatcgcgttc tgcaccgcct gcgcgccctt ctcggcggca
     7141 tgcagagaac tttccgccac cttggcggag tcgccggcgc tccttgacac ttcgctcatc
     7201 gagcgcgcca tctgcgtcac cgactggccg gtgccctcga tctcgacggc ctgcttttgc
     7261 gccgcctgca ggagctgcga ggcggtcgac tgcgcctccg ccgtcgccgt agtcacctgc
     7321 tgcgcggccg tgttgatgcc ggccaccagg cggcgcagct cgtcgatggc gtagttgatg
     7381 gagtcggcga tagcgccggt gatttcctcg gtcacctgcg cacgcgaggt gaggtcgccg
     7441 tcggccaggc tgccgatctc gtcgagcagc cgcagaatcg cctgctggtt cgccttgttc
     7501 tggcgctcgc tgtcctccgc ccggcgccgg ctgtctgcga tgtacaccac gccgatcagc
     7561 gccagcacgc cgagcgcgag cagcgccagc acgccgagcg cgataaagtt gaatcggcgc
     7621 ttggcgagat cggtttcgaa accggcgctc aggccttcgg ccgagcgcag cagcgcctcg
     7681 ctgtcgttga agatgtcgaa cgccgcgcgc ttggcgccga ccaggcgcgc ctggttgccc
     7741 agaatctcgc tgaccgcgcg ctgataatcg gcgaacgccg actgcagctc gcccagtttg
     7801 ccgcgcatct cggcgtcggc gacgcgggca atgcgcagcg cgtcgctgcc ttgcagcagg
     7861 ccctgtaccg tgtcgcggta ggtgttggtg tccttgccca gcaggaacgc gacctccggg
     7921 tcgacgatgt cctccgcgag catgatgttg gcgttcttgg ccatgcgctg cgtcagcatg
     7981 gtcagctgcg ccgtgatcgc gttctggcgc gcgctgccgc ccttctgcac gctcagcgcc
     8041 gccatctcgt cggtcagctc ctgcagcacg gggttgttgg cgttgatcgc gcgcaccgcg
     8101 ctgcccaggg cgacgaggtt gcgctcctcg tcgatcacca gcgcagcatt gcgctcggtc
     8161 ttgccccagc tgcggtccag cgcgttcagc gacggctgca cgtcccgcgg cgtgggcggc
     8221 aggtcgacgc cgccggtggt gccgccaaag gccagcagct tgacgatcgc cgcgaattcg
     8281 tcgcggctgc tgcgcagctg cttgaaggct tcggcgttgc cctgcgacgc ctgctgcgcg
     8341 gccttcgcaa ggcgctgcga cagcatctgc agcttgccga cgctggcgat gtagagggtg
     8401 ccgaacgttc cctgccgcgt gtcgtacacc accagggccg cgatgacggc gagcaggaag
     8461 accaacgccc cgatcagcag ctgcagctgc cgcgcgatcg gcagctggcc gatcaccggc
     8521 agtgcgctgg cgcccgcctc ggcgcgcttc gcgcccgccg gcgcgccggc gtcgctgccg
     8581 cgtccgagga acggcagttt gaatgcgcct atgttgattg ccatcgcagt ccccttatcg
     8641 caagaatttt ccgccgcgac cgatgatcgt caggtcgacg aatttcgagc ccgcgcggtt
     8701 ggtgggcgag tagctcacat agaagccccc gagatcggcg tcgttcatct tctccagcgc
     8761 cgcgatgtag ctctcgcgcg tcaggttcct gcccgcgcgg cgcaggccct ccaccgccac
     8821 cttggcggac aggaaccctt ccatcgcgct gaagttgtag tcggtgtggc cggacttttc
     8881 cgatagctgc tggtactcct tgaccaccgg caccgcccgc ccccagggga acggcaccac
     8941 ctgggagatg gccacgccga cgccgacctt gccgagcgca tccgagagcg ccttgctgcc
     9001 gacgaaggag acgttgtaga aggtcgcgtt gctgccggcg ctctgcatct gccggatgaa
     9061 ctccgcgcag gaggtgtacg cgctgatcat caccacggcg tccggccgcg ccgcgtggat
     9121 ctgcttgacg gcgtcctcga ccctgaccgt gttgcgctcg accgtgccga gcgccgtgat
     9181 cttcatgcca cgcttctgcg tggcgagctc cacgcccttc aggcccgcct ggccgtaggc
     9241 atcgttctgg tagaacaccg cgatcttctt gccgccgatc gacaccacct gctcgacgat
     9301 cttgtcggtc tcgtcgtagt agctggcgcg cacatggaac acgtagcggt tgaacggcac
     9361 ccgcaggacc tcggcccccg tgaacatccc caccagcggc accttggcct gcgtgaccac
     9421 cggcaggtgc gcgacgccgg taggcgtccc cacgtagccg atgagcgcga acaccttgtg
     9481 ctcgtcgatc agcttcttgc ttgcgacggg cgcgaccgcg gtctcgtagc gatcgtcttc
     9541 ggtgatcagg tcgatcttgc gtccgtggac gccgccccgc gcattgacgt agtcgaagta
     9601 ggccttgatg ccgttgcgca tctggatgcc cagctgcgcc gccggccccg agaacaccgc
     9661 ggcctggccg aacagcaccc tgtccgccgt cacgccggtc tctgcgggcg ccggaaacgc
     9721 gagcagcgcg ccgatcgcgc cgcccgccgc cattgctgcg attcgcttca tacctgcctc
     9781 catgagctgc ttctgtttca tgcgccgacg ctgagaaact ccgggtcctg gaccagctgc
     9841 gccacgtcca gctcgcgcca gtgcgcgccc tgcgggtcgt cgtattccgc cctgatccac
     9901 ggcgcgcgct gcggcgttgc ggcgcgctcc tgcagctgct ccgggttgcg caggccgagc
     9961 gagcggtcga ccagtagcgc gctgcccatg cgaaaacgct cgttcagcag cagcagccgc
    10021 gcctgctcgc cgggcatcgc cgcgccgccg ccgagaaacg cgccgatgtc caccacgctg
    10081 aacaggttgc cgcgaatgct cgccacaccc cggaaccagg ggcgcgtgag cggcaccggc
    10141 gtaatacccg gcaccggaat cacctcgccg gcgtcagtga gatccaccag ccagcgctcg
    10201 cctccgacct gcaggccgag cttggacgcc gcgcgcccgg cgcccgcgct ctgcagccgg
    10261 gccaccaggt cgcgctggta atcgcgaagt gaggtgcgtg ccgccactag ccgagcgccg
    10321 agatcttcgc cagcagctcg gcttgattca ccggtttgac gacgtaatcc cgggccccct
    10381 ggcgcatgcc ccagacgcgg tcggtttcct ggcccttggt ggtgcagatg atgaccggga
    10441 tgtccttcgt cgcgctgtcc ttgctcagcg tgcgggtggc ctgaaagccg ttttgccccg
    10501 gcatcaccac atccatcagg acgagatccg ggcgctgctg cttgaccgct tccagcgccc
    10561 gctcggcgct gtccgcggtg gtcacctggt agccgctttt cgccagcaga tcggtcaggt
    10621 actggcgatc ggtgggcgag tcatcgacga cgaggatgtt cttgatgggc atggtctcac
    10681 cttccgagag ggtggattag gaactggggg tcgcgggctt gagatactgg ttcacggtgt
    10741 cgatgagcgc ttccttggtg aacggcttgg tgaggtagcg gtccgacccg gcgaggcggc
    10801 cgcgggcgcg gtcgaacacg ccgtccttgc tcgacagcat gatcacgggc gttgccttga
    10861 gcttcgggtt ctgcttgatg agcgcgcagg tctggtagcc gtc
//
LOCUS       DBNHKE010000080         3118 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_119753, whole genome shotgun sequence.
ACCESSION   DBNHKE010000080 DBNHKE010000000
VERSION     DBNHKE010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_119753"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..228)
                     /locus_tag="AC16O9_05085"
     CDS             complement(43..228)
                     /locus_tag="AC16O9_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516022.1"
                     /translation="MIVLAGVGVAAFTGMLPGTSGSTDKDAMSAAPKADAPKATAAVP
                     AQPKPAQPEVAAPKPSR"
     gene            complement(342..1814)
                     /gene="lysS"
                     /locus_tag="AC16O9_05090"
     CDS             complement(342..1814)
                     /gene="lysS"
                     /locus_tag="AC16O9_05090"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR6516023.1"
                     /translation="MEEDNKLVEERREKLKALRAKGQAFPNDWRPQHLAGNLAQRYGG
                     LDKAALEQAGVAVTVAGRMMLKRVMGKASFATLQDVSGRIQVYVANDVAGEAEHEAFK
                     HWDLGDIVGVAGTLFKTNKGELTVNAKTLRLLVKALRPLPEKFHGLADQELRYRQRYV
                     DLIVNPESRNVFAARSRIVQSLRDTLIAEHYLEVETPMMQPIPGGAVARPFRTHHNAL
                     DIDLFLRIAPELYLKRLVVGGIERVFEINRNFRNEGISTRHNPEFTMLEWYCAYQDVE
                     YMIGLTETLVRNAALAATDGARLTYQGREIDFGQPFARLPIAEAIRRQGIDGDLRDRV
                     FLARTLTALGVEVKADQGWGALQLMLFEQVAEKHLVAPTFVLDFPAEVSPLARRKDSD
                     PGLTDRFELFVDTKEIANGFSELNDPEDQAERFREQAARKDAGDHEAMHYDADYIRAL
                     EVGLPPTAGAGLGVDRLVMLLTDSPSIRDVILFPHMRPEL"
     gene            complement(1814..2918)
                     /gene="prfB"
                     /locus_tag="AC16O9_05095"
     CDS             complement(join(1814..2836,2838..2918))
                     /gene="prfB"
                     /locus_tag="AC16O9_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR6516024.1"
                     /translation="MEAERLNTLADKLKDLKARSAELRRYLDFEAKEKRLLEVNKLLE
                     DPKVWSDPKRAQELGREKKSLEATVEALTEIDGGVTDAHELFELGQSENDEATLEGVE
                     TDAGALEAQVHQLEFRRMFSNPLDPNGCFVDIQAGSGGTEAQDWASMLERMYLRFCDR
                     KGYKVEILEQTEGEVAGIKSASLKVNGSYAYGTLRTETGVHRLVRKSPFDANARRHTS
                     FASVFVYPEVDETVEVELNPAEIRVDTYRASGAGGQHVNKTDSAIRMTHLPTGIVVQC
                     QNDRSQHRNRAEALAMLKSKLYELEMRKKEAERQKLEESKTDIGWGHQIRSYVLDQSR
                     IKDLRTGVEKGHTQAVLDGELDDFIEASLKQGV"
BASE COUNT          436 a         1061 c         1022 g          599 t
ORIGIN      
        1 ccgccgtccg ggtcttcttc ttcgacccga cctggctgcc gatcaccgcg acggcttggg
       61 cgccgcgacc tcaggctgcg cgggcttcgg ctgggccggg actgcagccg tggctttcgg
      121 cgcgtcggcc ttgggggctg ccgacatcgc gtccttgtcg gtcgagccgc tggtgcccgg
      181 cagcatgccg gtgaatgccg ctacgccgac gccggcgagc acgatcacag cgaccgcggc
      241 cgtcgcaaca agcggatgcc atgcccgatt ctgctgcgtc ttctatgagc ctctcgttca
      301 taccgtgacg cccctaccag cgccgatggt acacgttgcg cttacaactc cgggcgcatg
      361 tgtgggaaca agatcacgtc gcggatcgac ggactgtcag tgagcagcat gacgagccga
      421 tcgaccccga ggcctgcgcc tgccgtcggc ggcaggccta cttccagcgc gcggatgtag
      481 tcggcgtcgt agtgcatggc ttcatggtcg ccggcgtcct tgcgcgccgc ctgctcgcgg
      541 aagcgctcgg cttggtcctc ggggtcgttc agctcggaaa agccgttggc gatctccttg
      601 gtgtcgacga acagctcgaa gcgatcggtg agcccggggt ccgagtcctt gcggcgcgca
      661 agcggcgaca cttctgccgg gaaatcgagc acgaaggtgg gcgccaccag gtgcttctcg
      721 gcaacctgct cgaagagcat cagctgcagc gcgccccagc cctggtcggc cttcacctcg
      781 acgccaagcg ctgtgagcgt cctcgccagg aacacgcggt cgcgcaggtc gccgtcgatc
      841 ccttggcggc ggatcgcttc ggcaatcggc aggcgcgcga acggctggcc gaaatcgatc
      901 tcgcgcccct ggtaggtgag gcgcgcgccg tccgtcgccg cgagcgcggc gttcctgacg
      961 agcgtctcgg tgagcccgat catgtactcg acgtcctggt aggcgcagta ccactcgagc
     1021 atcgtgaact cggggttgtg ccgcgtggag atgccttcgt tcctgaagtt gcggttgatc
     1081 tcgaacacgc gctcgattcc gcccaccacc aggcgcttca gatacagctc gggcgcgatc
     1141 ctgagaaaca ggtctatgtc gagcgcgttg tggtgcgtgc ggaacggccg cgccaccgca
     1201 ccgcccggga tcggctgcat catcggcgtc tcgacctcga ggtagtgctc ggcgatcagc
     1261 gtgtcgcgca gcgactgcac gattctggag cgcgcggcga agacgttgcg cgactcgggg
     1321 ttgacgatca ggtcgacgta gcgctggcgg tagcgcagct cctggtcggc gaggccgtgg
     1381 aacttctccg gcaggggcct gagcgccttc accaggagcc gcagcgtctt cgcgttgacg
     1441 gtgagctctc ccttgttggt cttgaacagc gtccccgcca cgccgacgat gtcgcccagg
     1501 tcccagtgct tgaaggcctc gtgctcggct tcgcccgcga cgtcgttcgc cacgtacacc
     1561 tggatccggc cgctcacgtc ctgcagcgtg gcgaagctcg ccttgcccat cacgcgcttc
     1621 agcatcatgc ggcccgcgac cgtgaccgca acgcccgcct gctccagcgc cgccttgtcg
     1681 agaccgccgt agcgctgcgc caggttccct gcgagatgct gcggccgcca gtcgttggga
     1741 aaggcctggc ctttcgcgcg cagggccttc agcttctcgc ggcgctcctc gaccagcttg
     1801 ttgtcttctt ccatcacacc ccctgtttga ggctcgcctc gatgaagtcg tcgagctcgc
     1861 cgtcgagcac cgcttgcgtg tggccctttt ccacgccggt gcgcaggtcc ttgatgcgcg
     1921 actggtccag cacgtagctg cggatctggt ggccccagcc gatgtcggtc ttcgattctt
     1981 cgagcttctg gcgctcggcc tccttcttgc gcatctccag ctcgtagagc ttggacttga
     2041 gcatggcgag cgcctcggcg cggttcctgt gctgcgagcg gtcgttttgg cactgcacca
     2101 cgatgccggt gggcaggtgc gtcatgcgga tcgccgagtc ggtcttgttc acgtgctggc
     2161 cgccggcgcc cgaggcgcgg taggtgtcga cgcggatctc cgcggggttc agctccacct
     2221 ccacggtctc gtccacctcg gggtagacga agacactggc gaacgaggtg tggcgccgcg
     2281 cgttggcgtc gaagggggac ttgcgcacca agcggtgcac gccggtctcg gtcctgagcg
     2341 tgccgtaggc gtacgagccg ttcaccttca gcgaggcgct cttgatgccc gccacctcgc
     2401 cctcggtctg ctcgaggatc tcgaccttgt agcccttgcg gtcgcaaaag cgcaggtaca
     2461 tgcgctcgag catggaggcc cagtcctgcg cctcggtgcc gcccgagccc gcctggatgt
     2521 cgacgaagca gccgttgggg tcgagcgggt tcgagaacat gcgccggaac tcgagctggt
     2581 gcacctgcgc ttccagcgcg cccgcatcag tctccacgcc ttcgagcgtc gcctcgtcgt
     2641 tctcggactg gccgagctcg aacagctcgt gcgcgtccgt cacgccgccg tcgatctcgg
     2701 tgagcgcctc gaccgtggct tcgaggctct tcttctcgcg ccccagttcc tgcgcgcgct
     2761 tcgggtcgga ccagaccttc gggtcctcga gtagcttgtt gacctctaga aggcgctttt
     2821 ccttcgcctc gaagtcaaag gtacctcctc agctccgcgg accgggcttt caggtccttg
     2881 agcttgtcgg cgagggtgtt gaggcgttcg gcttccatgc gggccgcaat tataagcggc
     2941 ccccggcacg gcggctgcac tagaagccgc gcatcacgac cgcgccgatc gcccagtccg
     3001 gactcgagtc cgagaagccg accatggcgt aggcctgaag cttgagcgtc ttgtcgaacc
     3061 gatgcgagac gaacagcgtc gcttcgctca gcggatcacg tccactcagt acgggctg
//
LOCUS       DBNHKE010000081         5340 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_755277, whole genome shotgun sequence.
ACCESSION   DBNHKE010000081 DBNHKE010000000
VERSION     DBNHKE010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_755277"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..583
                     /locus_tag="AC16O9_05100"
     CDS             146..583
                     /locus_tag="AC16O9_05100"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR6516025.1"
                     /translation="MRAGFLAGILLLAGGYTWIAFAELAWLSSAGRLGPGFFPRFIGV
                     TLVALCAWSLVAELLDGSRERLSRHWRVTLVLALLSGLFVAALDVLGGLLSMIAYIAT
                     ALAYLNRRRSMQNALLAVLLPLAIYLLFRVWLNAAVPRGLLPF"
     gene            583..2082
                     /locus_tag="AC16O9_05105"
     CDS             583..2082
                     /locus_tag="AC16O9_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR6516026.1"
                     /translation="MDLFANLLLGFSVAVSPLNLVYLFLGVMIGMIVGIIPGFGPAAA
                     LAVLLPITFGMDPAGAVIMLAAIYYGAQYGGTITSILLNTPGESSSVASTFDGYPLAQ
                     QGRAGPALVMQAVASFVGGTLGVVLITLLAPPFTQLARGFGPPEFFLLVVMGLSTLIV
                     MIGGSWRLGLISALAGFALGTVGVDLETGNSRFTFGSAELIGGIDFIPVAIGLFGLGE
                     LYYAFYTGLHVSGTGGIVQLKKERSFWPEMRDYVETKWALARGSLLGFVVGVLPGAGA
                     TVASLMSYSLEKSVSRTPQKFGKGAMAGLVGPEAANNAASSGAMVPLLTLGIPGSAAT
                     AVLLAAFVLWGLTPGPLLMVQKPEFAWGLIASMYLGNMALLALNIFAIPLFVQMIKLP
                     YRILAPAVILICTIGTYSVNGSIIETWLMFGAGLVGFFMKLHGYSPAALVLALVLGPL
                     AEQSLRQTMTISSGSFGIFLDRPMSLAILCITVALLAAGILMRRGRRLD"
     gene            2126..>5340
                     /locus_tag="AC16O9_05110"
     CDS             2126..>5340
                     /locus_tag="AC16O9_05110"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIR6516027.1"
                     /translation="MGARKKGDDADLVTRIARAARGSLAPAHADLAERFARLYYAGAD
                     ARELAERDPKDLIGAAAAHLELGRRFDDGAAKVHVFNPRREEHGWQSTHTVVQVVGAD
                     MPFLVDSTTVEVNRLGLTLHLVLHPVLRVVRDAQGRLQEVAPPAAGADGRLESFMHLE
                     VDRQTDAARLQEIEQGLARVLADVAAAVADWKAMQQRLRERVDELGAAQPPLDAAEAR
                     EAQAFLAWLLDNHMTLLGYRDYDLVTENGEDVLRVVKGSGLGILRERADTTVSTSFAT
                     LPPETRARARQPEILVLSKANTRATVHRPGYLDYVGVKRFDAEGRVVGERRILGLYTH
                     TVYTANLDTIPVVRRKVAHVRERAGFLPASHNGKALASILEDYPRDELMQIEPDELYA
                     HAIAILQLGERQRTRLLVRRDAFGRFVTCLVFVPRDKYNTEQRTRFQQILAEAFNGTA
                     AEFTVSLSEAPLARVFMVIRTRPGTVPELDVRELEERIVAATRRWEDDLQSLLIERFG
                     EEQGNRLHARYGAAFPAGYREANDVRGALVDVEMMNTLGAPPALGMNLYRPPASEPGT
                     LRFRLVRTGAPPVLSDALPILENLGLRVLDDRPYRIDPAGGDTVWILDFGLALRGEAE
                     VALDRVRPLFHEAFEAMWNGWVEPDSLNQLVLAAELSARSIALLRAYSRYLRQAGFTF
                     SLDYVQQSLVAHAALSRALVRLFHARFEPGQACAEREQALVREIETALEQVPSLDDDR
                     ILRQFLAAIRATTRTNAFQSAAGGGRKTYLSLKFDPREVPGLPEPRPMFEIFVYSPRV
                     EGVHLRGGPVARGGLRWSDRREDFRTEVLGLMKAQMVKNAVIVPVGSKGGFVLKRAPP
                     PGDREALMQEGVECYKIFLRGLLDITDNLVGGKPRPPAQVVRHDGDDPYLVVAADKGT
                     ATFSDIANGVAQEYGFWLGDAFASGGSAGYDHKKMGITARGAWESVKRHFRELGRDTQ
                     SQPFTAAGIGDMSGDVFGNGMLLSRQIRLLAAFDHRHIFLDPDPDPENSFAERERLFA
                     LARSSWADYNAKLISKGGGVWPRTAKSVPLSPEARRALGVEA"
BASE COUNT          729 a         1911 c         1832 g          868 t
ORIGIN      
        1 aaggcctaca tcgacaagca gctccacacc gagaacgtgc tctacggcga ggatttccgt
       61 accttcctgc agaagaccca gggcgtcttt gccgacatcc tgaggaaatc gggcgccatc
      121 aagtagccgc gaccccggct gcaccatgcg cgcggggttc ctcgccggca tcctgctgct
      181 cgccggtggc tatacctgga tcgctttcgc cgagctcgcc tggctgtcct cggccggacg
      241 gctcggtccg ggtttcttcc cgcgcttcat cggcgtaacg ctggtcgccc tgtgcgcctg
      301 gagcctcgtc gccgagctgc ttgacggctc gcgcgagcgc ttgtcccggc actggcgcgt
      361 caccctggtg ctggcgctgc tctcggggct gttcgtcgcc gccctcgacg tgctgggcgg
      421 actgctgtcg atgatcgcct acatcgcgac ggccctggcg tacctcaacc gccggcgcag
      481 catgcagaac gcgctgctcg cggtgctgct gccgctcgcg atctatctgc tgttccgcgt
      541 ctggctgaac gccgccgtgc cgcgcgggct gctgccgttc tgatggacct gttcgccaac
      601 ctgctgctcg gcttttcggt cgcggtgtcg ccgctcaacc tcgtctacct gttcctcggc
      661 gtgatgatcg gcatgatcgt gggcatcatc cccggtttcg gcccggcggc cgcgcttgcg
      721 gtcttgctgc cgatcacgtt cggcatggat cccgccggcg ccgtgatcat gcttgccgcg
      781 atctactacg gggcgcagta cggcggcacc atcacctcga tcctgctcaa cacgccgggc
      841 gagtcgtcgt cggtggcgag caccttcgac ggctatcccc tggcgcagca ggggcgcgcc
      901 ggcccggcgc tggtcatgca agcggtcgcc tccttcgtcg gcggcacgct cggggtggtg
      961 ctcattacgc tgctcgcgcc gcccttcacg cagcttgcgc gcggcttcgg gccgccggaa
     1021 ttcttccttc tcgtggtcat gggcctgtcc acgctcatcg tcatgatcgg cggcagctgg
     1081 cggctggggc tgatctcggc gctcgccggt ttcgcgctcg gcaccgtcgg cgtcgacctg
     1141 gaaaccggca acagccgctt caccttcggc tcggcggagc tgatcggcgg catcgatttc
     1201 atcccggtgg cgatcggcct gttcggcctc ggcgagctgt actacgcgtt ctacaccggg
     1261 ctgcatgtct cgggcacggg cggcatcgtg cagctgaaga aggaaaggag cttctggccg
     1321 gagatgcgcg actacgtcga gaccaagtgg gcgctggcgc gcggatcgct tctcgggttc
     1381 gtggtcggcg tgctgcccgg cgccggcgcg accgtggcct cgctcatgtc ctactcgctg
     1441 gaaaaatccg tgtcgcgcac gccgcagaag ttcggcaagg gcgccatggc gggactggtc
     1501 ggccccgaag cggcgaacaa cgccgcctcg tccggcgcga tggtgccgct gctcaccctc
     1561 ggcatccccg gctcggcggc caccgcggtc ctgctcgcgg cgttcgtgct gtggggactg
     1621 acgccggggc cgctgctcat ggtgcagaag cccgagttcg cgtggggcct gatcgccagc
     1681 atgtacctcg gcaacatggc gctgctcgcg ctcaacatct tcgccattcc gcttttcgtg
     1741 cagatgatca agctgcccta ccgcatcctc gcgccggcgg tgatcctgat ctgcaccatc
     1801 ggcacctaca gcgtgaacgg cagcatcatc gagacctggc tcatgttcgg cgccgggctg
     1861 gtcggcttct tcatgaagct gcatggctat tcgcccgccg ccctggtgct cgcgctcgtg
     1921 ctcggcccgc tggccgagca gtcgctgcgc cagacgatga cgatctcgag cggctcgttc
     1981 ggcatcttcc tcgaccgccc catgtcgctc gccatcctct gcatcaccgt cgcactgctg
     2041 gccgcgggca tcctcatgcg gcgcggacgc cgtctagact agcgcgatca atccgcggcc
     2101 gcgcgccggg catatacttg cgcgcatggg cgcgagaaag aaaggcgacg acgccgatct
     2161 cgtgacgcgc atcgcgcgcg cggcgcgcgg cagcctggca ccggcgcacg ccgatctcgc
     2221 cgagcgcttc gcgcgcctgt actacgccgg cgccgacgcg cgcgagctcg ccgagcgcga
     2281 cccgaaggac ctaatcggcg cggccgcagc ccacctcgag ctcgggcgcc gcttcgacga
     2341 tggcgccgcc aaggtgcacg tgttcaaccc ccggcgcgaa gagcacggct ggcagagcac
     2401 gcatacggtg gtgcaggtgg tgggcgccga catgccgttc ctggtcgact cgacgaccgt
     2461 cgaggtgaac cggctcgggc tcacgctgca cctcgttctc cacccggtgc tgcgcgtggt
     2521 gcgcgatgcg cagggacgct tgcaggaggt cgcgccgccg gccgccggcg ccgatggacg
     2581 gctggagtcg ttcatgcacc tggaggtcga ccgccagacc gatgccgcgc gcctgcagga
     2641 gatcgagcag ggactggcgc gggtgctggc cgacgtggcc gccgcagtgg ccgactggaa
     2701 agccatgcag cagcgcctac gcgagcgggt cgacgagctc ggcgcggccc agccgccgct
     2761 cgacgcggcc gaggcgcgcg aggcgcaggc gttccttgcc tggctcctcg acaaccacat
     2821 gacgctgctc ggctatcgcg attacgacct cgtcaccgag aacggcgagg acgtgctgcg
     2881 cgtggtgaag ggctcgggcc tgggcatcct gcgcgagcgt gcggacacca ccgtctcgac
     2941 gagcttcgcc acgctgccgc cggagacgcg cgcgcgcgcg cgccagcccg agatcctggt
     3001 cctgtccaag gccaatacgc gcgccaccgt gcaccgcccc ggctacctcg actacgtcgg
     3061 cgtgaagcgc ttcgacgccg aagggcgcgt ggtcggcgag cgccgcatcc tcggtttgta
     3121 cacccacacc gtatacacgg cgaacctcga cacgatcccc gtcgtgcggc gcaaggtggc
     3181 gcacgttcgc gagcgcgccg ggttcctgcc ggcgagccac aacggcaagg cgctcgcttc
     3241 gatcctcgag gactacccgc gcgacgaact gatgcagatc gagcccgacg agctctacgc
     3301 gcacgccatc gccatcctgc agttgggcga gcgccagagg acgcgcctgc tggtgcggcg
     3361 cgacgcgttc ggtcgcttcg tcacctgcct ggtgttcgtg ccgcgcgaca agtacaacac
     3421 cgagcagcgc acccggttcc agcagatcct cgccgaggcg ttcaacggca cggcggccga
     3481 gttcaccgtg agcctgtcgg aagcgccgct tgcgcgcgtg ttcatggtga tccgcacccg
     3541 cccggggacc gtgcccgagc tggacgtgcg cgagctcgag gagcggatcg tcgccgccac
     3601 gcggcgctgg gaggacgacc tgcagtcact gctcatcgag cgcttcggcg aggagcaggg
     3661 caaccgcctg cacgcgcgct acggcgcggc cttccccgcg ggctatcgcg aggcgaacga
     3721 cgtgcgcggt gcgctggtcg acgtcgagat gatgaatacg cttggcgcgc cgccggcact
     3781 cggcatgaac ctgtaccggc cgccggccag cgagcccggc acgctgcgct tccggctggt
     3841 gcgcaccggc gcgccgccgg tcctgtcgga cgcgctgccg atcctcgaga acctcggcct
     3901 gcgcgtgctc gacgaccgac cctaccgcat cgaccccgcg ggcggcgaca cggtgtggat
     3961 cctcgatttc ggcctcgcgc tgcgcggcga ggccgaggtg gcgctggatc gcgtgcggcc
     4021 gctgttccac gaggcgttcg aggccatgtg gaacggctgg gtcgagcccg acagcctgaa
     4081 ccagctggtg ctggcggccg agctctcggc gcgcagcatc gcgctgctgc gggcctacag
     4141 ccgctacctg cgccaggcgg ggttcacctt cagcctcgac tacgtgcagc agtcgctggt
     4201 cgcgcacgcg gcgctgtcgc gcgcactggt gcggctgttc catgcgcgct tcgagcccgg
     4261 gcaggcgtgc gccgaacgcg agcaggcgct ggtacgcgag atcgagaccg cgctggagca
     4321 ggtgcccagc ctggacgacg accgcatcct gcgccagttc ctcgccgcga tccgcgccac
     4381 cacgcgcacc aacgccttcc agagcgctgc cggcggcggc cgcaagacct acctctcgct
     4441 caagttcgat ccgcgcgagg tgccggggct gcccgagccg cggccgatgt tcgagatctt
     4501 cgtctactcg ccgcgcgtcg agggcgtgca cctgcgcggc gggccggtgg cgcgcggggg
     4561 cctgcgctgg tcggaccggc gcgaggactt ccgcaccgag gtgctcgggc tgatgaaggc
     4621 gcagatggtc aagaacgcgg tcatcgtgcc ggtcggctcc aagggcggct tcgtgctcaa
     4681 gcgcgcgccg ccgccgggcg accgcgaggc gctgatgcag gaaggcgtcg agtgctacaa
     4741 gatcttcctg cggggcttgc tcgacatcac cgacaacctg gtcggcggca agccgcggcc
     4801 gcccgcgcag gtcgtgcgcc atgacggcga cgacccgtac ctggtggtgg ccgccgacaa
     4861 gggcaccgca accttctccg acatcgccaa cggggtggcg caggagtacg gcttctggct
     4921 cggcgatgcg ttcgcctcgg gcggctcggc gggctacgac cacaagaaga tgggcatcac
     4981 cgcgcgcggc gcctgggaat cggtcaagcg ccatttccgc gagctcggcc gcgacacgca
     5041 atcgcagccg ttcaccgcgg cggggatcgg cgacatgtcg ggcgacgtgt tcggcaacgg
     5101 catgctgctg tcgcgccaga tccggctgct cgccgcgttc gaccaccgcc acatcttcct
     5161 cgatcccgat cctgatcccg agaacagctt tgccgagcgc gagcgcctgt tcgcgctggc
     5221 gcgctcctcc tgggccgact acaacgcgaa gctgatctcc aagggcggcg gcgtctggcc
     5281 gcgcaccgcc aagtcggtgc cgctctcgcc ggaggcgcgg cgcgcgctgg gcgtcgaagc
//
LOCUS       DBNHKE010000082         5864 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1389974, whole genome shotgun sequence.
ACCESSION   DBNHKE010000082 DBNHKE010000000
VERSION     DBNHKE010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1389974"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC16O9_05115"
     CDS             <1..269
                     /locus_tag="AC16O9_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6516028.1"
                     /translation="AVAATTAIGVYFWSGSIGLVYVISISMVISMVAAGLAGALVPIL
                     LRRFGQDPATASSIILTTVTDVVGFFSFLGIATLFAGMLGAMPG"
     gene            279..1901
                     /locus_tag="AC16O9_05120"
     CDS             279..1901
                     /locus_tag="AC16O9_05120"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6516029.1"
                     /translation="MNDLLIRGATVYDGTGAPGRVADVAVKDGRIAAPGAARETLDAG
                     GLALMPGIVDVHTHYDAQVTWDPKLSPSPALGVTTAIMGNCGFGIAPCPRGSRETILK
                     NLSVVEGMDVHALLEGTRWDFETFPQYLDALERIHPYANVGVLAGHSTIRSAVMGEEA
                     SSRRQPTPDELRKMQDMVREALAAGAAGFASSFSPNHSGWGGRPMPSTICEDAELRAL
                     VAPLKAAGRGIFVVAAGSRATPEYLEQMAADTGRPMFMVTVLTMHSSQNPGRAMTYYE
                     RCAAALKRGREVYIHANCQPLSFDFTLKEPYPFMALAAFDRVKAAPPAELAAIYKDPA
                     FRRDFRDSLRNPKPGVLFSGDWSTVEIDGVPLPQLAGAQDPLEWFCERPLEQQMIAKL
                     FQNDDAGVAPLLKHEAGVIALSDAGAHLIYFCDAGFGLHLLAHWVRGNGTFTLEEAVR
                     RLTSDPARKYRIPERGVIRAGAWADLLLFDPRTVGLSNLRRVQDLPAGGTRMVRDAVG
                     VHGVWVNGARVHDGRDYCAAQGPGRVLREFGR"
     gene            complement(1904..2881)
                     /locus_tag="AC16O9_05125"
     CDS             complement(1904..2881)
                     /locus_tag="AC16O9_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6516030.1"
                     /translation="MAAQATQFASEWRREIVTRSPAMERVLARARLSAAGDAGLLLQG
                     ASGTGKRLLARAVHAASARFGRPFVALHCGALPEPLLESELFGHVKGAFPGAVHDHAG
                     LLQSAQGGTLFLAEVSELPLALQAKLLRALQERQARAVGATQALPIDVRVIAGTRREL
                     AEEMAAGRFRAELYYHLNTVTLALPPLAERREDVAPLAAHFLAAAAERCGKRIEGIAP
                     AALPPLRTAPWPGNVRQLREVIERCVAQCGGRRLSAALVTAALAPADDDFAALDAARR
                     EFESEYLTRLLRITDGNVAQAAKLAKRQRADFLALLARYALDPKAFKPL"
     gene            2977..5409
                     /locus_tag="AC16O9_05130"
     CDS             2977..5409
                     /locus_tag="AC16O9_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516031.1"
                     /translation="MAGVALGVALGFGVHLVNRAAVEELAAGVRTLAGEADLEVQGGR
                     TGFAESLYPKLARLPGVAAASPALELDAGLPGGGSIRVFGIEPLRAAQLQPALLAHQP
                     QLRTEILRPDTVLLSQAAAERLRLGTGGRLALISGLATVELEVIGTLPASALPGIAAL
                     ADIATVQWRLARVGELNRVGLRLAPGADRDAVRARAAALVPAGVHVTRVETSAASGAA
                     LSRAYRVNLNVLALVALVTGGFLVFSAQALEVARRRGEHALLRVLGLTRGALARLVLA
                     EAALVGALGAALGLALGYAFAAAAMRASGGDFGAGMFRGLAPEVSFAPLAALAYFAAG
                     VAVALAGAALPAADAARAAPAQALKAGDEQRILARAPALIPALSFLGAGALLALAPPV
                     DGIPLAGYASVACLLVGGILLMPALSRRLWQALPLARRPALALAAAQLRGAPGQAMVS
                     LAAIVASFSLMVAMAIMVASFRDSVDQWLTALLPAELYVRTTHAGETAFLEPRFEARV
                     RRLPQVARAEFLRSARVTLDPARPAISLLARDVPEGGFATRLPLVGARHTPREGEPPP
                     AWVSEAVAERYGAAPGKRLALPMGGRSREFVVAGVWRDYARQHGAIVIERPTYAQLTG
                     DARANDAALWLKPGVDAADAVRALRALPGGEQLDFGTPGEIREVSLAIFDRSFAVTYA
                     LEAVAVLVGLIGLSSSLGALVLARRREFGVLRHLGMTRREIGAMLATEGALLALAGAV
                     AGLVLGAAISLVLVYVVNPQSFGWSMDLHPPWSFLAALAGVLLVLAMLTAWVSGREAM
                     GMAPVRAVKEDW"
     gene            5406..>5864
                     /locus_tag="AC16O9_05135"
     CDS             5406..>5864
                     /locus_tag="AC16O9_05135"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR6516032.1"
                     /translation="MRRRQFLLALMAAPLARAGEVDYAAVVPGRRLRFPRDHGAHPEY
                     RTEWWYATGWLERGGERLGFQVTFFRARTPWETRNPSKFAPRQILLAHAAIADPRHGR
                     LRHDERAARDAFGLAGAATRTTEAWIDDWRFVLERGVYRARIAARDFVLDL"
BASE COUNT          683 a         2138 c         2188 g          855 t
ORIGIN      
        1 tggcggtggc ggcgaccacc gcgatcggcg tgtacttctg gagcggctcg atcggcctgg
       61 tgtacgtgat ttcgatctcg atggtgatct cgatggtcgc ggcggggctc gccggcgcgc
      121 tggtgccgat cctgctgcgg cgcttcgggc aggatccggc gacggcctcg tcgatcatcc
      181 tcacgacggt gaccgacgtg gtggggttct tctcgttcct cgggatcgcg acgctgttcg
      241 cggggatgct gggggcgatg ccgggctaga ctgacgggat gaacgacctg ctgatccgcg
      301 gcgctaccgt ctacgacggc accggcgcgc cgggccgcgt cgccgacgtg gccgtaaagg
      361 atggccgcat cgccgcgccg ggcgccgcgc gagagaccct cgacgccggc gggctcgcac
      421 tcatgcccgg catcgtcgac gtgcatacgc actacgacgc gcaggtcacc tgggatccga
      481 agctctcgcc ctcgcccgcc ctgggcgtga ccaccgccat catgggcaac tgcggcttcg
      541 gcatcgcgcc ctgcccgcga ggctcgcgcg agacgatcct gaagaacctt tcggtggtcg
      601 agggcatgga cgtacacgcg ctgctcgaag gcacgcgctg ggatttcgag accttcccgc
      661 agtacctcga cgcgctggaa cgcattcatc cctacgccaa cgtcggcgtg ctcgcggggc
      721 actccaccat ccgcagcgcg gtgatgggcg aggaggcctc cagccgccgg cagccgacgc
      781 cggacgagct gaggaagatg caggacatgg tgcgcgaggc gctggccgcg ggcgccgcgg
      841 gcttcgcgtc gtcgttctcg cccaaccaca gcggctgggg cgggcgcccg atgccctcga
      901 cgatttgcga ggacgccgaa ttgcgcgcgc tggtggcgcc gctgaaggcg gcgggcaggg
      961 gcatcttcgt cgtcgccgcc ggctccaggg cgacgcccga gtacctggag cagatggccg
     1021 cggacaccgg gcggccgatg ttcatggtga ccgtgctcac catgcattcg agccagaacc
     1081 cgggccgcgc catgacctac tacgagcgct gcgccgcggc gctgaagcgc ggacgcgagg
     1141 tctacatcca cgccaactgc cagccgctgt ctttcgattt cacgctgaag gagccctatc
     1201 cgttcatggc gctcgccgcg ttcgaccgcg tgaaggcggc gccgccggcg gagctcgcgg
     1261 cgatctacaa ggacccggcg ttccgccgcg acttccgcga cagcctgcga aatccaaagc
     1321 cgggcgtgct gttctcgggc gactggagca cggtggagat cgacggcgtg ccgctgccgc
     1381 agctcgccgg cgcgcaggac ccgctcgagt ggttctgcga gcgcccgctc gagcagcaga
     1441 tgatcgcgaa gctgttccag aacgacgacg cgggcgtggc gccgctgctc aagcacgagg
     1501 ccggcgtgat cgcgctctcg gacgcgggcg cgcacctgat ctatttctgc gacgcgggct
     1561 tcggcctgca tttgctcgcg cactgggtgc gcggcaacgg cacctttacg ctcgaggagg
     1621 cggttcggcg cctgacgtcc gacccggcgc gcaagtaccg catcccggag cgcggcgtga
     1681 tccgcgccgg ggcctgggcc gacctgctgc tgttcgatcc gcgaaccgtc ggcttatcca
     1741 acctgcgccg cgtgcaggac ctgcccgccg gcggcacgcg catggtgcgc gacgcggtgg
     1801 gcgtgcacgg cgtttgggtg aacggcgcgc gggtgcacga cggccgcgac tattgcgcgg
     1861 cacagggtcc gggccgcgtg ctgcgcgagt tcgggcgcta gacctagagc ggcttgaacg
     1921 ccttcggatc gagcgcgtag cgcgccagca gcgcgaggaa gtcggcgcgc tggcgcttgg
     1981 cgagcttcgc cgcctgcgcg acgttgccgt ccgtgatgcg cagcagccgc gtcaggtatt
     2041 cgctctcgaa ctcgcggcgc gccgcgtcga gcgccgcgaa gtcgtcgtcg gcgggggcga
     2101 gggcggccgt caccagcgcc gccgacaggc gccggccgcc gcactgcgcg acgcacctct
     2161 cgatcacctc ccgcagctgg cgcacgttgc cgggccaagg cgcggtgcgt agcggcggca
     2221 gcgccgccgg cgcgatgccc tcgatccgct tgccgcagcg ctcggcggcc gcggccagga
     2281 agtgcgccgc gagcggcgcg acgtcctcgc gccgctccgc aagcgggggc agcgccagcg
     2341 tcaccgtgtt caggtggtaa tacagctccg cgcgaaagcg cccggcggcc atttcctcgg
     2401 cgagctcgcg ccgcgtcccg gcaatcacgc gcacgtcgat gggcagcgcc tgcgtcgctc
     2461 cgacggcgcg cgcctggcgt tcctgcagcg cgcgcaacag tttcgcctgc agcgcgagcg
     2521 gcagctcgct cacttcagcc aggaagagcg tgccgccctg cgccgactgc agcaggcccg
     2581 cgtgatcgtg taccgcgccg gggaaggcgc cctttacgtg gccgaagagc tcggactcga
     2641 gcaggggctc gggcagcgca ccgcaatgaa gcgcgacgaa cgggcggccg aagcgcgcgc
     2701 tggcggcgtg cacggcacgc gccaagaggc gcttgcccgt gccgctcgcg ccttgaagga
     2761 gcaggcccgc gtcgcccgcg gccgacaagc gcgcgcgcgc cagcacgcgt tccatcgccg
     2821 ggctgcgcgt gacgatctcg cgccgccatt cggacgcaaa ctgcgtcgcc tgcgcggcca
     2881 tggtgctgcc attctagccg ctatactcgc cgcatccgcc tcttccttgc cctcatcgca
     2941 cgactcgcgc aacgccgcgg gcgcacgctg ctctccgtgg ccggcgtcgc cctgggcgtc
     3001 gcgctcggct tcggcgtgca cctggtcaac cgcgccgccg tcgaggagct cgccgccggc
     3061 gttcgcaccc tcgccggcga ggccgatctc gaagtgcagg gcggtcgcac cggctttgcc
     3121 gagtcgctgt atccgaagct cgcgcgcctg ccgggggtgg cggcggcgag cccggcgctc
     3181 gagctcgacg cggggctgcc gggcggcgga tcgatccgcg tcttcggcat cgagccgcta
     3241 cgcgccgcgc agctgcagcc ggcactgctc gcgcaccagc cccaattgcg cacagagatc
     3301 ctgaggcccg atacggtgct cctgtcgcag gccgcggccg agcggctgcg cctgggaacc
     3361 ggcggccggc ttgcactcat ctcggggctc gccacggtgg agctggaggt gatcggaacc
     3421 ctgccggcct cggcgctgcc gggaatcgcc gcgcttgccg acatcgccac ggtgcagtgg
     3481 cggctggcgc gggtgggcga gctcaatcgc gttggcctgc ggctggcgcc gggcgcagac
     3541 cgggacgcgg tgcgggcgcg cgccgccgcc ctggtgccgg cgggggtgca cgtcactcgc
     3601 gtggaaacca gcgccgcgag tggcgcggcg ctgtcgcgcg cctaccgtgt caacctcaac
     3661 gtgctggcgc tggtggcgct cgtcaccggc gggttcctgg tgttctccgc gcaggcgctg
     3721 gaagtcgcgc gccggcgcgg cgagcacgcg ctgctccggg tgctcgggct gacgcgcggc
     3781 gcgcttgcgc gcctggtgct cgccgaggcc gccctggtcg gcgcgctggg cgcggcgctc
     3841 ggccttgcgc tcggctacgc ctttgccgcc gcggcaatgc gcgcctcggg cggcgatttc
     3901 ggcgccggga tgttccgcgg tctcgcgccc gaggtcagct tcgcgcccct ggcggcgctc
     3961 gcctacttcg ccgccggcgt cgcggtggcg ctcgcgggcg cagcgctgcc ggccgccgac
     4021 gcggcgcgcg cggcgccggc gcaggcgctg aaggccggcg acgagcagcg catcctggcg
     4081 cgcgcgcccg cgctcatccc cgcgctctcg ttcctcggcg ccggcgcgct gctcgcgctg
     4141 gcgccgcccg tggacggcat tccgcttgca ggctacgcgt cggtcgcctg cctgctcgtg
     4201 ggcggcatcc tgctcatgcc tgcgctgtcg cggcgactgt ggcaggcgct gccgctcgcg
     4261 cggcggccgg cactggcgct ggccgccgcg cagctgcgcg gggcgcccgg gcaggcgatg
     4321 gtgagcctcg ccgccatcgt cgcgagcttc tcgctgatgg tggcgatggc gatcatggtg
     4381 gcctcgttcc gcgattccgt ggaccagtgg ctcaccgcgc tgctgccggc ggagctctat
     4441 gtgcgcacca cgcatgcggg cgagacggcg ttcctcgagc cgcgtttcga agcgcgcgtg
     4501 cgccgcctgc cgcaggtggc gcgcgcggag ttcctgcgca gcgcgcgcgt gacgctggac
     4561 ccggcgcggc cggcgatttc gctgctggcg cgcgacgtac cggagggtgg attcgcgacc
     4621 cgcctgccgc tcgtcggggc acgccacacg ccgcgcgagg gcgagccgcc gccggcgtgg
     4681 gtcagcgagg ccgtggccga gcgctacggc gccgcgcccg gcaagcggct cgcgctgccg
     4741 atgggcggcc gcagccgcga gttcgtcgtc gccggcgtgt ggcgcgacta cgcgcgccag
     4801 cacggcgcca tcgtcatcga gcgcccgacc tacgcccagc tgaccgggga cgcgcgcgcc
     4861 aacgacgcgg cgctctggct caagccgggc gtcgacgcgg cggacgcggt gcgcgcgctg
     4921 cgcgcgctgc ccggcggcga gcagctggat ttcggcaccc cgggcgagat ccgcgaggtg
     4981 tcgctcgcga tcttcgaccg cagcttcgcc gtgacctacg cactggaggc cgtggcggtc
     5041 ctggtggggc tgatcgggct ctcgagcagc ctcggcgcgc tggtgctcgc gcgccgccgc
     5101 gagttcggcg tgctgcgcca cctcggcatg acgcgccgcg agatcggcgc gatgctcgcc
     5161 accgagggcg cgctgctggc gctggcgggc gccgtggccg gcctggtcct cggcgcggcg
     5221 atcagcctgg tgctcgtgta cgtggtcaat ccgcagtcct tcggctggag catggacctg
     5281 catccgccgt ggagcttcct ggccgcgctt gccggggtcc tgctcgtgct cgccatgctg
     5341 accgcgtggg tctcggggcg cgaggccatg gggatggcgc cagtgcgtgc agtgaaggag
     5401 gattggtgag aaggcgccag tttctcctcg cgctcatggc cgcgccgctc gcgcgcgccg
     5461 gcgaggtcga ctacgcggcc gtggttcccg gccggcgcct gcgctttccg cgcgaccatg
     5521 gcgcccaccc cgagtaccgc accgagtggt ggtacgccac cggctggctc gagcgcggcg
     5581 gcgagcgcct cggcttccag gtgacgttct ttcgcgcgcg cacgccctgg gagacgcgca
     5641 atccgagcaa gttcgcgccg cgccagatcc tgctcgcgca cgcggcgatc gccgacccgc
     5701 gccacgggcg gctgcgccac gacgagcgcg cggcgcgcga cgcgttcggt ctcgccggcg
     5761 ccgccacgcg caccaccgag gcctggatcg acgactggcg cttcgtgctc gagcgcggcg
     5821 tgtatcgcgc ccgcatcgcg gcgcgcgatt tcgtgctcga cctc
//
LOCUS       DBNHKE010000083        31544 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1390314, whole genome shotgun sequence.
ACCESSION   DBNHKE010000083 DBNHKE010000000
VERSION     DBNHKE010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 31544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31544
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1390314"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="AC16O9_05140"
     CDS             <1..640
                     /locus_tag="AC16O9_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6516033.1"
                     /translation="GVFRESRSRDRVFFVESEGAAAGEVRNVFVNSVQHGRTGVMFSR
                     RGLVETAPNGDRFIVMLDGRRYEGVPGEADYRVMEFERYEARVESAAGEEPVPTHKSL
                     STLALLGNPTHVNHGELVWRIGVPLSALILALLAIPMSFVNPRAGRSVNLLFALLAYL
                     VYQNLLSVSQARVAQGRLDFSLGWWLVHAAVLAVLLVLFAQRMTLRRSRLRG"
     gene            645..1730
                     /gene="lptG"
                     /locus_tag="AC16O9_05145"
     CDS             645..1730
                     /gene="lptG"
                     /locus_tag="AC16O9_05145"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR6516034.1"
                     /translation="MTGATRTLNRYLAREVLAAVSFILLGFLGLFAFFDLLGEFRDLG
                     KGEYNLRQVFTYVLLSTPTHAYELLPVAVLIGTLYVLAHLANNSEFTVMRAAGMSPGQ
                     ASGMLLRTGLVFAALTFAIGEWVAPAAEEAAQQVRMRAFSSAIGQDLHSGLWFRDERA
                     FINVREAREASRLNGVRIYEFDADYRLDSITSAALGEYLEQGAWRLSKVVQTRFTGEG
                     PRTTHLAQTQWRTAVTPELIGVLVVDPERMSAWSLYRYTQHLAGNRQKTGRYEIALWK
                     KLFYPIATLVMMALALPFAYMHARSGMVGLKVFLGIMLGIFFHMLNGLFAHIGLLQDW
                     PPFAAAAVPGTAFFLAAVLMMWWVERR"
     gene            complement(1727..2113)
                     /locus_tag="AC16O9_05150"
     CDS             complement(1727..2113)
                     /locus_tag="AC16O9_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6516035.1"
                     /translation="MTPGLARRLGGMLYEALLLFAVAFFAGSAFHFASGAAPLEGWLL
                     RAHQAFLAAVFAAYFLWCWLRGGQTLAMKAWRVRLVNVTPGRALLRFACALVLVPTGI
                     SIIWSLADRDGQFLHDRLAGTRLVLA"
     gene            complement(2110..2517)
                     /locus_tag="AC16O9_05155"
     CDS             complement(2110..2517)
                     /locus_tag="AC16O9_05155"
                     /inference="COORDINATES: protein motif:HMM:NF022748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HIR6516036.1"
                     /translation="MAQAARLAAALAAALLLTAAPAGAAPEPDRRWQQLSAEQRTILA
                     PLEAEWDRLEPVQRVKWIGVAKRYPKMTPVGQKRVQSNMKKWAKLTPAQRKQARENYR
                     RISKVPPEQRGDLRQQWDEYQSLPPPKKPAPAK"
     gene            complement(2484..2867)
                     /locus_tag="AC16O9_05160"
     CDS             complement(2484..2867)
                     /locus_tag="AC16O9_05160"
                     /inference="COORDINATES: protein motif:HMM:NF023699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HIR6516037.1"
                     /translation="MNESQFALRVRQALNQGLHLDARVAARLRAAREQALAHQRPEAA
                     AGLALASNALGRLGGARGLSLRVLLPLLVLAVAFATTYAWQQQQRAAELEELDAQLLS
                     SDLPIDAYLDQGFAAWLKRRASQQP"
     gene            complement(2872..3441)
                     /locus_tag="AC16O9_05165"
     CDS             complement(2872..3441)
                     /locus_tag="AC16O9_05165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6516038.1"
                     /translation="MASRSELSAFLEGVERRAFKQALYAVRDEQAALDIVQDAMLRLA
                     EKYGERPGTELPMLFQRILQNAIRDWYRRQRVRAGWVTLFSSLLPGHAGDDDADPLET
                     LAAAGESNAEDAPPQHLERSQVVEIIEQEIEKLPARQREAFLMRYWEELDVADTARAM
                     GCSEGSVKTHCSRAAHALAVALKARGITL"
     gene            3599..5293
                     /locus_tag="AC16O9_05170"
     CDS             3599..5293
                     /locus_tag="AC16O9_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HIR6516039.1"
                     /translation="MELSGAQIVVRCLQEEGVEYVFGYPGGAVLFIYDELFKQDKVRH
                     VLVRHEQGAAHAADGYSRSSKKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVLTGQV
                     PTHAIGQDAFQECDAVGITRPCVKHNFLVKDVKDLATTIKKAFYLAQSGRPGPVLVDI
                     PKDVTTQLCEFQYPDVVAMRSYNPVKKGHAGQIKKAVQLLLESERPMVYTGGGVVLSN
                     AAPQLRALVQQLGLPCTNTLMGLGAYPGTDRQFLGMLGMHGTYQANMAMQHCDVLLAI
                     GARFDDRVIGNPAHFAQNPRKIIHIDVDPSSISKRVRVDVPIVGNIPDVLDDVLKLLK
                     DHKPLAIEPWWKQIEAWRARDCLRFDRTSKIIKPQFVLEKLYEVTGGEAIVTSDVGQH
                     QMWAAQFYKFDRPRQWINSGGLGTMGFGLPAAMGAQLANPGATVACVTGESSIQMCLQ
                     ELSTCKQYRLPIKIVNLNNRYMGMVRQWQQFFHGNRYSESYMDALPDFVKLAEGYGHR
                     GVLIEKPADVEPALRDAFKRKDELVFLDFLTDQAENVYPMVPGGKGITEMILSEEL"
     gene            5293..5784
                     /gene="ilvN"
                     /locus_tag="AC16O9_05175"
     CDS             5293..5784
                     /gene="ilvN"
                     /locus_tag="AC16O9_05175"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR6516040.1"
                     /translation="MRHIISILVENEAGALSRISNMFSARGYNIESLTVAPTEDASMS
                     RMTVVTVGSEDVIEQITKQLNKLVEVVKVVDLSEAEHIEREMMLIKVRAGAKEREDMK
                     RMADIFRGRIIDVSDNTYTIELTGEGRKLDAFIQALDQGAIIETVRTGASGIGRGNRV
                     LRV"
     gene            5845..6861
                     /gene="ilvC"
                     /locus_tag="AC16O9_05180"
     CDS             5845..6861
                     /gene="ilvC"
                     /locus_tag="AC16O9_05180"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR6516041.1"
                     /translation="MKVYYDKDADLSLVKGKKVTIIGYGSQGHAHALNLQESGVKVTV
                     GLRKGGASWDKAKKGGVKVAELGEAIKGADIVMILLPDEHHAQIYRDYVEPNIKKGAS
                     LAFAHGFNIHYGLIQPRADLDVWMVAPKAPGHTVRSTYAAGGGVPMLIAVHRNPSKKA
                     REFALSYAAAIGGGKAGIIETSFKEETETDLFGEQAVLCGGCVELVKAGFDTLVDAGY
                     APEMAYFECLHELKLIVDLMYEGGIGTMNYSISNNAEYGEYVSGPKVIDAGTRARMKE
                     ILGRIQTGEYAKEFVLENRAGAPELLSRRRMTAELPIEKVGARLREMMPWIRKNRLVD
                     TSKN"
     gene            6865..7521
                     /locus_tag="AC16O9_05185"
     CDS             6865..7521
                     /locus_tag="AC16O9_05185"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR6516042.1"
                     /translation="MKPPYPHPLIAREGWPFLGAAVAAALLIWIFAGGWWSVPVWLAA
                     LFVLQFFRDPAREVPDDPRAVVAPADGRVVAVEKARDPWLERDALKISVFMNVFNVHS
                     NRAPVDGAVRQVWYHAGSFVNAALDKASVENERNALWLRTADGADVTCVQIAGLVARR
                     ILCYVGAGAELQRGQRFGFIRFGSRVDVYLPLEAQAVAAIGDKVYAAETVLAKLAGHA
                     "
     gene            7514..8299
                     /gene="pssA"
                     /locus_tag="AC16O9_05190"
     CDS             7514..8299
                     /gene="pssA"
                     /locus_tag="AC16O9_05190"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR6516043.1"
                     /translation="MPDPKEPQRQDYLPGLVPPRGGALRRRGIYLLPNLFTTAALFAG
                     FYAIVQAMNGRFGESAIAIFIAGLLDGLDGRVARLTRTQSEFGAEYDSLSDMVSFGAA
                     PALVLYEWALKDLGRFGWIAAFVYCAGAALRLARFNVMIDVVDKRYFQGLPSPAAAAL
                     VAGFVWLAHDNKIPLTEYGLQWVAACVALYGGLTMVSNVPFYSFKDVNFRKSVPFWAM
                     LLIVLGFVLVSSDPPIVLFLLFCAYGLSGYVMWALGKRPRPRP"
     gene            8502..10049
                     /locus_tag="AC16O9_05195"
     CDS             8502..10049
                     /locus_tag="AC16O9_05195"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR6516044.1"
                     /translation="MSKGKLIIFDTTMRDGEQSPGASMTKDEKLRIARALERMRVDVI
                     EAGFPASSNGDFEAVRAIANVVKDCTVAGLCRANDRDIQRGIDALRGAKSWRIHTFIA
                     TSELHMAKKLRMTPEQVLEQTRLSVRYAKNACDDVEFSPEDGSRSDPEFLCRVLEAAI
                     KEGATTINIPDTVGYAVPEQFGQMIRSLRERVPNSDKAVWSVHCHNDLGMAVANSLAA
                     VVIGGARQIECTINGLGERAGNTSLEEVVMAVRTRKDFFDLETGIDTTQIVPTSRLVS
                     QITGFVVQPNKAVVGANAFAHASGIHQDGVLKARETYEIMTAADVGWTNNKIVLGKLS
                     GRNAFKQRMKEIGIELESEDAYNKAFQRFKDLADKKAEIFDEDLQALVTQEAGKSGDE
                     HWRLVTMNQASGMGEKPHARIVLAENGAEKKAESQGDGPVDATFKAIETVAKSGAELL
                     LYSVNAVTQGTESQGEVTVRLSKGGRIVNGVGADTDIVVASAKAYISALNKLESKVER
                     LNPQHQL"
     gene            10073..10489
                     /gene="rnk"
                     /locus_tag="AC16O9_05200"
     CDS             10073..10489
                     /gene="rnk"
                     /locus_tag="AC16O9_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HIR6516045.1"
                     /translation="MSEGKLKRPEITVATDDLERLEGLLGELRGGAPEIADGLRSELD
                     RARIVEPSEIPPDVVTMNSTVRFTDEESGKEFRRTLCWPEEVTGGEDKVSILAPLGSA
                     LLGLSVGQRIDWPVPGGRMARIRILDVVSKPGRAGG"
     gene            10495..11340
                     /locus_tag="AC16O9_05205"
     CDS             10495..11340
                     /locus_tag="AC16O9_05205"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6516046.1"
                     /translation="MANARRTWLFVPGADAAAHEAAARSGADVLIQELEDFTPPERRP
                     QARRLAATLYERWRAAGALAGVRVNPLETCGREDLAAVLAGRPDIVLMSKVDAPEQVR
                     ALAAAAPGVELVPNIESARGLLRTAAIARADRRVSALLVASEDMVADLGTSRSRGGEE
                     LAYVRQRFLLECRAAGVEAIDCPYTFSDAKGAAADARWARRMGYRMKSLVDPSHARAV
                     NRVFTPNVAAARRIVAAFEKARAQGKERARVNGALIEVPIYAAAKRVLESAARTPPST
                     RRTRG"
     gene            complement(11279..11620)
                     /locus_tag="AC16O9_05210"
     CDS             complement(11279..11620)
                     /locus_tag="AC16O9_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HIR6516047.1"
                     /translation="MIRAAHLAACASLGFLILLCLGWELWLAPLRPGGSLLALKAAPL
                     ALAIPGILNGRRYTYQWSSMLILAYFAEGAVRAWSERGAAQWLASAEIALSLAFFAST
                     VAYARLTRAPA"
     gene            complement(11617..12210)
                     /gene="wrbA"
                     /locus_tag="AC16O9_05215"
     CDS             complement(11617..12210)
                     /gene="wrbA"
                     /locus_tag="AC16O9_05215"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR6516048.1"
                     /translation="MADILVLYYSVGGAVRRLAELVAEGVERVPGAAARLRTVPRVST
                     VTRQAQPQIPESGAPYCELADLESCAGLALGSPTRFGDMAAPLKHFLDGTGSLWLKGG
                     LAGKPACVFTSTGSMHGGQETTLLAMMVPLLHHGMVIVGTPYTQAELNATRSGGTPYG
                     PSHFAGIADDQPVTDAERALCIAQGRRLAQVALKLAQ"
     gene            12232..13428
                     /locus_tag="AC16O9_05220"
     CDS             12232..13428
                     /locus_tag="AC16O9_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR6516049.1"
                     /translation="MRRPDLRALLEFCLALARRFRDDRGIQTAGSLTFTTLLALVPLV
                     TVALAMSTAFPVFEQAMEALGRYVSGQLLPEGGARITQQFAAFAAKAGRLTAVGLAFL
                     AVTALMLILTVDEVMNRIFRVQRRRGLAQRLLMYWAVLSLGPLLIGASLSMTSFLVGS
                     SLGLLNLGWMTRGVLGLMPFLFTCAALALLYLVVPYRRIEARHALAGAIVAGILFEFA
                     KRGFALYIAKFPTYSLIYGAFATVPIFLLWLYLSWVVVLFGATLSAMLPGYRGGYGRH
                     ADAPGRELIDATAVLRVLALARRDEGALALVALARRAKLPPERCERVLERCAAQGWAA
                     RTDRERWLLVRDADTIQLGAVVRAFAMDPEALRAGAPAALAEQLLRAEDSLTMTVQDL
                     LQEQDA"
     gene            13425..14129
                     /gene="pyrF"
                     /locus_tag="AC16O9_05225"
     CDS             13425..14129
                     /gene="pyrF"
                     /locus_tag="AC16O9_05225"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR6516050.1"
                     /translation="MSAIDDRGRLIAALDVPSAGDARALAERLGDAVRFYKIGLELFS
                     AGGYFELSDWLRGRGARVFADLKLYDIPETVRRAVANLRASGASFLTVHAERSIMEAA
                     AREKGDLKVLAVTVLTSFDQKSLAEMGYAGSVEDLVLHRARAALESGCDGVIASGLEA
                     PKIKAAFGGRLLVVVPGVRPAGAAAQDQKRVVDVKQAFASGADYIVVGRPIRDAADPR
                     AAAIAIQDAIAGALQR"
     gene            14197..16017
                     /gene="typA"
                     /locus_tag="AC16O9_05230"
     CDS             14197..16017
                     /gene="typA"
                     /locus_tag="AC16O9_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR6516051.1"
                     /translation="MPRALRNLAIIAHVDHGKTTLVDKLLRAAGTFAAHERLEERVMD
                     SNDLERERGITILAKNCAVRYEGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLKPIVVVNKVDRPGARAQWTVNQTFDLFDKLGASEEQLDF
                     PVVYASALEGWASLESGARGGDMKPLFEAILAHVPMRDEDAEGPLQLQICSLDYSSYV
                     GKIGIGRIRRGRLKAAQEVLVLNGPDAAPLKAKVNQVQMFEGLQRVQVAQAEAGDIVL
                     VNGIEELGIGTTLCHPEHPEAMAPLKVDEPTLTMNFLVNASPLAGREGKYVTSRQIRE
                     RLERETKSNVALRVEYPADTDTFIVHGRGELHLTILLENMRREGYEIAVSRPRVVYKE
                     IGGVRCEPFEVLTVDVEEANQGGVMEELGRRRGELLEMHPDGKGRVRLDYRISARGLI
                     GFQGEFMTLTRGTGIMSHVFDEYAPARTEEMAERRNGVLVSQDDGAAVAYALWKLQER
                     GRMFVNPGDPVYEGMIIGIHSRDNDLVVNPIRGKQLTNIRASGKDEAIDLTPPIGLTL
                     EYAVEFIADDELVEITPKSIRLRKRFLVESERRRAERAAA"
     gene            complement(16154..17098)
                     /locus_tag="AC16O9_05235"
     CDS             complement(16154..17098)
                     /locus_tag="AC16O9_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6516052.1"
                     /translation="MLLKTQFKPAQTRAPLERLIAAAGEVILGKETQVRLALACLIAR
                     GHLLIEDLPGVGKTTLAHVLAKTLGMHFQRIQFTSDMLPADIIGVSVYERDSGAFKFH
                     PGPIFAQLILADEVNRATPKTQSALLEAMEEHQVTVETETRKLPEPFFVIATQNPSEQ
                     VGTFPLPESQLDRFLMRIELGYPDRGAERALLAGADRRDLLASLDPCMSPAELVELQS
                     DAQRVHVAPALLDYVQAIVEYTRRSPDYVAGLSPRAALALVHSARAWALLEKRDKVIP
                     EDVQTILPGVAAHRLRPAHDTARRVDVGAQLLAAVPIP"
     gene            17132..18061
                     /locus_tag="AC16O9_05240"
     CDS             17132..18061
                     /locus_tag="AC16O9_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR6516053.1"
                     /translation="MTVAYVTHADCLKHEMGAWHPERPERLTAIEDQLIASGLGQFLQ
                     RHEAPLATDEQLARVHPMEYVRAIREAAPTEGTVHLDPDTAMNPHSLQAALRAAGAVV
                     LATDLVLRGEVDGAFCGVRPPGHHACRARPMGFCIFNNVAVAARHALEAHGLERVAII
                     DFDVHHGNGTEDIFQNDARVLMASTFQHPFYPYSGTEDPAPNMVNVPLRAGAGSKQFR
                     AAVSEAWLPALEGFRPQLVIFSAGFDAHADDDMAMLSFTDQDYAWVTQQVKAVADRHA
                     GGRIVSALEGGYALSALGRSVVQHLRVLAGLDA"
     gene            18164..19009
                     /locus_tag="AC16O9_05245"
     CDS             18164..19009
                     /locus_tag="AC16O9_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6516054.1"
                     /translation="MRLAHLAVALALAALVLPLHAQSLKVSAIPDESPTELLRKFKPL
                     GAYLEKQTGMKVDWTPVNDYPAVVEGLAAKRIDLAWLGGFTFVQARIRTGDAIPIAQR
                     VEDEKFISYYIVPKASRAKTLQDLKGKTFAFGSPASTSGHLMPRFFLLKAGLNPDKDF
                     TRLAFSGAHDATVAFVASGRAEAGALNASVYDKLIEKGDANARSVRILQTTPPYYDYN
                     WTVRGSLDPALVSKLTAAFLNLDPKNPAHKEILDLQRATRFVATKLENYKGIEEAARS
                     AGLLK"
     gene            19023..19856
                     /locus_tag="AC16O9_05250"
     CDS             19023..19856
                     /locus_tag="AC16O9_05250"
                     /EC_number="7.3.2.2"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR6516055.1"
                     /translation="MKLDVDALHLVLPGGQRALAGVSLGVARGERVAVIGPSGAGKTS
                     LLRILGLALRPSAGRLALADVDPWVLPARALQQLRGRIGMIHQAPPLPPRQRLVTAVL
                     AGRLGAWGLWRGLASLAYPADIAGARDALGRLDLAERVFDRCDRLSGGQLQRVGVARV
                     LYQKPDLILADEPVSALDPQLADQAVGELHREAQSRGVTLVASLHAVDLALKWFPRIV
                     GLRDGAVVFDCPPVRVSDAMLHELYATESQTLPVQGGAPLAHPEPLSRASRRGAQPLC
                     R"
     gene            19847..20683
                     /locus_tag="AC16O9_05255"
     CDS             19847..20683
                     /locus_tag="AC16O9_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhnE/PtxC family ABC transporter permease"
                     /protein_id="HIR6516056.1"
                     /translation="MPLAGAAAAPRDPALPARVALTLVVLLLLWPALELTEFDPGKLF
                     EPGNLQMMGRFLGTFFPLALGADFLGLVLRSALETLALATAGMALAVALAAPLSLAVS
                     ESLSVSRIGPAPLAGLRRLLRAPLRLLLVVMRSIPEIVWALLFVRAVGLGPAAGILAI
                     GINYAGMLGKVYFEIYEAGDTRPARAVLEAGGGRLHAFFYGILPVSLPELVSYTVYRW
                     ECAIRASVVMGFVGAGGLGQQMELSMRMLAGGEVATMLVAFFLLVMLADAISALLRKV
                     LT"
     gene            20680..21465
                     /gene="phnE"
                     /locus_tag="AC16O9_05260"
     CDS             20680..21465
                     /gene="phnE"
                     /locus_tag="AC16O9_05260"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR6516057.1"
                     /translation="MTPGAFVWRPWAVLGAFVLAALASLLYLGVDFIALFGRDSVRQM
                     AEFARGFAPPDTSPEFLGRAAVGAAETLAISVVGTLLAALGGAFLALPASGRWSRWLR
                     PPARLVLNVLRSIPELVWAVFMVLAAGLGPFAGCLALAVHTAGVLGRLFAETLENAPP
                     EPARALRDAGAGATLAFLYGTLPVALPQFTSYALYRWEYNIRMAAVLGFVGAGGLGQM
                     LYVSLSVFKTHEAATLLLAMIAMVTLVDLLSAWLRRFLGRSGA"
     gene            complement(21422..22408)
                     /gene="senB"
                     /locus_tag="AC16O9_05265"
     CDS             complement(21422..22408)
                     /gene="senB"
                     /locus_tag="AC16O9_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HIR6516058.1"
                     /translation="MARIALVTPAGAGTRNGNRHTALRWAAFLRRAGHRVSVSLAWDG
                     APCELLLALHARRSHDAVARYRAAHPRGALAVALTGTDLYRDLPGSADARRSLALADR
                     LIVLQDEALRRLAPALRRKTRVVFQSAAPRARRQPPAAVFRVAVIGHLREEKDPFRAA
                     AALARLPRDAAVEVVHVGAALRARMEREARRRMRGEPRYRWLGGLPHARALAWLARSH
                     LLVVSSRMEGGANVIAEAARIGTPVLASRVPGNIGMLGRGYPGYFPLGDERALARLMA
                     RAAREPEFYRRLRRALAARRHLFAPAAERAALVHALRGLTRPSGRGSGAARR"
     gene            22432..23496
                     /gene="senA"
                     /locus_tag="AC16O9_05270"
     CDS             22432..23496
                     /gene="senA"
                     /locus_tag="AC16O9_05270"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HIR6516059.1"
                     /translation="MLERQLREARARTDLMTRDLDGERLLGPMLDIVNPPLWEIGHVA
                     WFQERWCLRMAQSGAPIDSILEGADALYDSAAVAHDTRWDLPLPGPQATRAYMAEVLE
                     RVCARLQREPDNPALEYYAQLCLAHEDMHAEAFHYTRQTLGYPDPGAGAPAPVAAAGD
                     EAFAGGRFERGARPGEGFVFDNEKWAHEIDVAPFRMACAPVTNAEYLEYVEQGGGAPP
                     RYWKREDGAWLQRRFDRWLPLAPAGPVLHVSALEAEGYCRWAGRRLPSEAEWEFAARS
                     GRLAAGGVWEWTASAFLPYPGFSPDPYEEYSRPWFGTHRVLRGASFSTPPRLVRPAFR
                     NFYMPQRADVFCGFRTCAVA"
     gene            complement(23499..23765)
                     /locus_tag="AC16O9_05275"
     CDS             complement(23499..23765)
                     /locus_tag="AC16O9_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6516060.1"
                     /translation="MDIKDLRYFVAVYEEGGFARAGVRLDTVQSNVSARIRRLELFLD
                     AQLFERRRRGVAVTEKGEKLYRHAKRVIALVEETPAAVRGKEAA"
     gene            complement(23985..25037)
                     /gene="mnmH"
                     /locus_tag="AC16O9_05280"
     CDS             complement(23985..25037)
                     /gene="mnmH"
                     /locus_tag="AC16O9_05280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR6516061.1"
                     /translation="MKAPSHSVAMQPSILTAFDAIIDARSPAEFALDHVPGALSLPVL
                     DDAERARIGTMYTQTSPFAAKRLGAALVARNIARHLETALAERPRQWRPLVYCWRGGK
                     RSAAFAHVLREVGWPAQTLEGGYRAYRRGIVEQLAALPAAFELRVVHGATGSGKSRLL
                     GALARAGAQVLDLEALAAHRGSVLGELPDQPQPSQKMFESRLYAALAALDRRRAVYVE
                     GESRKIGQLQVPEALISAMRAAECVRLEADLEARVALLMEEYRHFFAAPEMLAAQLEC
                     LAPLHGRERVDAWKALAARGAWPQLVARLLEEHYDPAYRRSAERNFARLPQARVLRVA
                     AADEDVFAGLARTLSA"
     gene            25058..25690
                     /locus_tag="AC16O9_05285"
     CDS             25058..25690
                     /locus_tag="AC16O9_05285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516062.1"
                     /translation="MTTEPDPRRALEQYRRHAQGYDASARRTMRLRRRAVDWLELAPG
                     ERVLDVACGTGLSFPLLAERVGAAGQVVGVELSPEMMRQARARAAPLRAQLRLIEASM
                     EDAVLEGPFDAVLFNYTHDVLQSPRALANIFAHVKPGARVVAAGVKHPPAWLLPLRLL
                     RLWKARPYLTTFRGLDRPWAPLEAYVEGMEVEPVLLGTNYIARARARIHA"
     gene            25687..26736
                     /gene="selD"
                     /locus_tag="AC16O9_05290"
     CDS             25687..26736
                     /gene="selD"
                     /locus_tag="AC16O9_05290"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - L-selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR6516063.1"
                     /translation="MNAPEKIRLTEFSHGGGCGCKIAPAVLSEILAATPIRGLPEALM
                     VGTETADDAAVYRLNDSQALVATTDFFTPIVDDPYDFGRIAATNALSDVYAMGGRPIM
                     ALAIVGMPLEKLPLSVIQRILEGGESVCASVGIPIAGGHSIDTPEPIYGLVALGLVHP
                     DRVKKNSGARAGDVLVLGKPLGVGILSAVLKKGMISAEGYRQMIECTTRLNTPGEALS
                     EMPGVHALTDITGFGLAGHLLGICRGSKLAAEVKLDAVPVIAEALDWVKQGVATGASD
                     RNWKGYGADVELPAGAPDWMRALVTDPQTSGGLLVACEPQAERAVLRAFADRGFTEAR
                     TIGRLTAGAPRVRVG"
     gene            complement(26723..27301)
                     /locus_tag="AC16O9_05295"
     CDS             complement(26723..27301)
                     /locus_tag="AC16O9_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR6516064.1"
                     /translation="MCFSLGAWGKTILVFGDSLSAAYGIAAKRGWVTLLAERLERERL
                     DYSVVNASISGETTAGGVSRLPRALARHKPAIVILELGANDGLRGLPAAEMQKNLAAM
                     IGMSKKAGAKVLLTGMRVPPNYGPEYAEAFAAVFEALARRHATAFVPFFLEGIADNLR
                     LFQPDRIHPTEEAQPLLLENVWKALRPLLSRP"
     gene            27300..27980
                     /locus_tag="AC16O9_05300"
     CDS             27300..27980
                     /locus_tag="AC16O9_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516065.1"
                     /translation="MNKQATSVLAARGIGKTVKSGEADLVILREIDLAVMPGEVVAIV
                     GASGSGKSTLLALLAGLDTPTTGTVELAGADLFALDEDARAELRGRAVGFVFQSFQLL
                     PSLTALENVMLPLELAGRADAEDKAREILARVGLAERLQHYPKHLSGGEQQRVALARA
                     FVVRPQLLLADEPTGSLDVKSGEAVIELLFQLNREAGTTLVMVTHDEHLAQRCTRIVR
                     LAAGRIAA"
     gene            27980..30469
                     /locus_tag="AC16O9_05305"
     CDS             27980..30469
                     /locus_tag="AC16O9_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516066.1"
                     /translation="MPLALRMLGRDWRAGELRVLAAALVVAVASITSVAFFADRVSRA
                     LVRDAHQLLGADLVLVSDHPWKAGIGEEIARRGLQRAEAVNFISMARGDAGSQLAGVK
                     AVTYNYPLRGRLRIAAAPNAPDAPAATGPARGSVWLDERLVSALGAPVGARLKLGNAE
                     FAVAAVITLEPERGANFFNIAPRLMMHAADVPATGLVQTGSRVWFYLYAAGDPAAVAA
                     FEAWARTRLERGQRVENLESGRPEVRASIERAQRFLGLTALLAAILAGVAIALATRRF
                     VERHLDACAVMRCMGATQKRLLGLYGAEFLVLGAAASLIGCALGYLAHGAIAAALGEL
                     LRAQLPPASIAPALQGFSVGLVLLLGFALPPLVQLKNVPAVRVIRREAGAPRGGTVAI
                     YAAGLAALSALLVWQAGDLRLGLYVIGGFGVAVLVFAAVAFGALRAVSASAVARRLGA
                     RSSALRYGLANLRRHARGNAVQVASLALGLTAVLLLTFTRNDLVDAWRRSAPPDAPNR
                     FLIGVQPDQVAEVRGFLAGAGIAEAELHPMVRGRLAAVNGKAVSEADYAEERAKRLVE
                     REFNLSYSDELPGHNQIAAGRWYAPGAKEMSVEEGIAERLGWKLGDELTFSVGAEQFT
                     ARISSLRKLRWDSMKINFFVIVPPALLEGYPASFVSAFRVAPGEERALDELAKRFPNL
                     TLVDVSAALRQAQRVIDQVINAVQFVFLFALGAGLLVLYSALVATEDERRREAAVMRV
                     YGASRAHVTGSQRVEFLAMGLVAGILATLGAAAIGQLLARQVFELDLPPSAGLWLAGP
                     LAGVVLLSLNAWLSARKVLTASPALTLRDSV"
     gene            30478..>31544
                     /locus_tag="AC16O9_05310"
     CDS             30478..>31544
                     /locus_tag="AC16O9_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6516067.1"
                     /translation="MAEIAALAVALAALGVVVWLALRLQGAAEKKHREMLTDLHDGLT
                     RQGDRLGAQLGELREAVAAKLDARLDQISLRVNERLDEGFRKTNETFVNVMQRLATID
                     EAQKKIETLTGSVVSLQELLGDKRSRGAFGEVQLEALVRNVLPTQAFEMQYTLSNGTR
                     VDCALRLPAPTGLVCVDSKFPLENYKLMLDPKANELERAGAQKAFRADVKRHVDAIAS
                     KYIIEGETSDGAVMFVPAEAVFAEIHAYHAEVVDYAIGKRVWIVSPTTLMAVLNTARA
                     VLKDVETRKQIHVIKEALARLAVEFGRFDERMKELARHIRQAAEDVDRIQTTGGKISQ
                     QFQRIEAAEVDDAPVAEVVRL"
BASE COUNT         4675 a        10925 c        11234 g         4710 t
ORIGIN      
        1 gggcgtgttc cgcgagtcgc gctcgcgcga ccgcgtgttc ttcgtcgagt cggagggcgc
       61 cgccgccggg gaggtgcgga acgtgttcgt caactcggtg cagcacgggc gcaccggcgt
      121 catgttcagc cgccgcggcc tcgtcgagac cgcacccaac ggcgatcgct tcatcgtgat
      181 gctcgatggc cggcgctacg aaggcgtgcc gggcgaggcc gattaccgcg tcatggaatt
      241 cgagcgctac gaggcgcgcg tcgagtccgc ggctggcgag gagccggtgc cgacgcacaa
      301 gagcctgtcg acgctcgcgc tgctggggaa tccgacgcac gtaaaccacg gcgagctggt
      361 gtggcgcatc ggcgttccgc tttcggcgct gatcctggcg ctgctcgcta tcccgatgag
      421 cttcgtcaac ccgcgagcgg ggcgctcggt gaacctgctg ttcgcgctgc tcgcctacct
      481 tgtgtaccag aacctgctga gcgtcagcca ggcgcgggtg gcgcaaggcc ggctcgactt
      541 ttccctggga tggtggctgg tgcacgcggc cgtgctcgcg gtgctcttgg tgctgttcgc
      601 gcagcgcatg acgttgcggc gctcgaggtt gcggggttga gacgatgacg ggcgcaaccc
      661 gtacgctaaa ccgctacctc gcgcgcgaag tgctggcggc ggtgagcttc atcctgctcg
      721 ggtttctcgg cttgttcgcc tttttcgacc tgttgggcga attccgcgac ctgggcaagg
      781 gcgagtacaa cctgcgccag gtgttcacct acgtgctgct gtccacgccg acgcacgcct
      841 atgagctgct gccggtggcg gtgctgatcg gcacgctcta cgtgctggcg cacctcgcca
      901 acaattccga gttcaccgtg atgcgcgccg ccggcatgtc gccgggccag gcgagcggca
      961 tgctgctgcg caccggcctc gtgttcgctg cgctcacctt cgccatcggc gagtgggtgg
     1021 cgccggccgc ggaagaagcg gcgcaacagg tgcgcatgcg cgcgttctcc tctgccatcg
     1081 gccaagatct gcactcgggg ctgtggttca gggatgagcg cgcgttcatc aacgtgcgcg
     1141 aagcgcgcga agcgagccgg ctgaacggcg tgcgcatcta cgagttcgac gccgattacc
     1201 gcttggacag catcacctcg gcagcgctcg gcgagtactt ggagcagggc gcctggcgcc
     1261 tgagcaaggt ggtgcagacc cgcttcaccg gggaaggccc gcgcacgacg caccttgcgc
     1321 agacgcaatg gcgcaccgcc gtaacgcctg agctgatcgg cgtgctggtg gtcgatcccg
     1381 agcgcatgtc ggcgtggagc ctgtaccgct acacccagca cctggcgggc aaccgccaga
     1441 agacggggcg ctatgagatc gcgctgtgga agaagctctt ttacccgatc gccacgctgg
     1501 tgatgatggc gctcgcgctg ccgttcgcgt acatgcacgc tcgctccggc atggtgggcc
     1561 tgaaggtatt cctcggcatc atgctgggca tcttcttcca catgctgaac ggcctctttg
     1621 cccatatcgg cctgctgcag gactggccgc cgtttgccgc cgccgccgtg ccggggacgg
     1681 cgttctttct cgccgcggtg ctgatgatgt ggtgggtcga gcggcgctag gcgagcacga
     1741 gccgcgtgcc cgccagccga tcgtgcagga actggccgtc gcggtcggcg agggaccaga
     1801 ttatcgagat gccggtcggc acgaggacga gcgcgcaggc gaagcgcagc agcgcgcggc
     1861 ccggcgtcac gtttaccagg cgtacgcgcc aggccttcat cgccagcgtc tggccgccgc
     1921 gcagccagca ccacaagaag taggcggcga acacggcggc gagaaacgcc tggtgcgcgc
     1981 gcagcagcca gccttcgagc ggcgccgcgc ccgaagcgaa atggaaggcg gagccggcga
     2041 agaaggcgac ggcaaacagc agcagcgcct cgtacagcat gccgcccagg cggcgcgcga
     2101 ggcccggcgt cacttcgccg gcgcgggctt cttgggcggc ggcaacgact ggtactcgtc
     2161 ccactgctgg cgcaggtcgc cgcgctgctc gggcggcacc ttgctgatgc ggcggtagtt
     2221 ctcgcgcgcc tgcttgcgct gcgccggtgt gagcttggcc catttcttca tgttcgactg
     2281 cacgcgtttt tggccgacgg gcgtcatctt cggatagcgc ttggcgacgc cgatccactt
     2341 cacgcgctgc accggttcga ggcgatccca ttcggcctcg aggggcgcga ggatcgtccg
     2401 ctgctccgcg ctcagctgct gccagcgacg gtccggctcc ggggcggcgc ccgccggtgc
     2461 cgccgtgagc agcagcgccg ccgctagggc tgctgcgagg cgcgccgctt gagccacgct
     2521 gcgaaaccct ggtccaggta ggcgtcgatc ggcaggtcgc tggagagcag ctgcgcgtcc
     2581 agctcctcaa gctcggccgc gcgctgctgc tgctgccacg cataggtggt ggcgaacgcg
     2641 acggcgagga ccaggagcgg cagcagcacg cgcagcgaca ggccgcgcgc gccgccgagg
     2701 cgacccagcg cgttgctggc aagggcgagc cctgccgcgg cctcaggccg ctggtgcgcg
     2761 agcgcctgct cgcgcgcggc gcgcagccgc gcggccaccc gggcatccag gtgcagcccc
     2821 tggttcaacg cctggcggac gcggagggcg aattgcgatt cgttcatggg cttacagcgt
     2881 aataccgcgc gccttgaggg ctacggccaa ggcatgcgct gcccgtgagc agtgcgtttt
     2941 cacgctgccc tcggagcagc ccatggcgcg agcggtgtcg gcaacgtcca attcctccca
     3001 ataacgcatg agaaacgcct cccgttgacg cgccggcagc ttctcgatct cttgttcaat
     3061 tatttcaaca acttgcgacc gctcaaggtg ctgcggcgga gcgtcttcgg cgttcgactc
     3121 gccggccgcg gccaaagttt cgagcggatc ggcgtcgtcg tcaccggcgt gacccggcag
     3181 caacgacgaa aacagcgtca cccaccccgc gcgtacccgc tgccgccggt accagtcgcg
     3241 gatcgcgttc tgcaggatgc gctggaacag catcggcagc tccgtgccgg ggcgctcgcc
     3301 gtacttctcc gccagccgca gcatcgcgtc ctgcacgatg tcgagcgccg cctgctcgtc
     3361 acgcacggca tacagggcct gcttgaaggc gcgccgctcc acgccctcga ggaaggccga
     3421 aagctcgctg cgcgaggcca gtggttctcc cttcctccga cgcccggggt gcggtttgcg
     3481 ggcgatgtta ccaaaagcgc cccgaacagc cgcggcccgt tgcttgaccg caatgcaaca
     3541 agtcattaat gttcaacgtt ttccccagcc atcgcgggtc atggaccgaa tcaagggagt
     3601 ggagttgagc ggcgcccaga tcgtggtgcg ttgcctgcag gaagaaggcg tcgagtacgt
     3661 attcggctac ccgggcgggg ccgtgctgtt catctacgac gagctcttca agcaggacaa
     3721 ggtgcgccat gtgctggtgc gccacgagca gggggcggcc cacgcggcgg acggctactc
     3781 gcgctcctcg aagaaagtcg gcgtgtgcct ggtgacgtcc ggccccggcg tgaccaacgc
     3841 ggtgaccggc atcgccaccg cctacatgga ctcgatcccg atggttgtgc tcaccggtca
     3901 ggtgccgacg cacgccatcg gccaggacgc cttccaggag tgcgatgcgg tcggcatcac
     3961 gcgaccttgc gtcaagcaca acttcctggt gaaggacgtg aaggacctcg ccaccaccat
     4021 caagaaggcc ttctacctcg cgcaaagcgg gcggcccggc ccggtgctgg tggatatccc
     4081 gaaggacgtc acgacgcaac tgtgcgagtt ccagtacccg gacgtcgtgg caatgcgttc
     4141 gtacaacccg gtgaagaagg gccacgccgg gcagatcaag aaggcggtgc agctgctgct
     4201 cgagtccgag cggccgatgg tgtacacggg cggcggcgtc gtcctttcga acgccgcgcc
     4261 gcagttgcgc gcgctggtcc agcagctcgg cctgccgtgc accaacacgc tcatgggcct
     4321 gggcgcctac cctggcaccg accgccagtt cctgggcatg ctgggcatgc atggaacgta
     4381 tcaggccaac atggcgatgc agcactgcga cgtgctgctg gcgatcggcg cgcgcttcga
     4441 cgaccgcgtg atcggcaacc cggcgcactt cgcgcagaac ccccgcaaga tcatccacat
     4501 cgacgtcgac ccgtcgtcca tctccaagcg ggtcagggtc gacgtgccga tcgtcggcaa
     4561 catccccgac gtgctggacg acgtgctcaa gctgctgaag gaccacaagc cccttgccat
     4621 cgagccgtgg tggaagcaga tcgaggcctg gcgcgcgcgc gactgcctgc gcttcgaccg
     4681 cacttcgaag atcatcaagc cgcaattcgt gctggagaag ctctacgagg tgacgggcgg
     4741 cgaggccatc gtcacctcgg acgtcggcca gcaccagatg tgggcggcac agttctacaa
     4801 gttcgaccgg ccgcggcaat ggatcaactc cggcggcctc ggcacgatgg gctttggcct
     4861 cccggcggcg atgggcgcgc agctcgccaa ccccggtgcc acggtggcct gcgtcaccgg
     4921 cgagtcctcg atccagatgt gcctgcagga gctctccacc tgcaagcagt accggctgcc
     4981 gatcaagatc gtcaacctca acaacagata catgggcatg gtgcgccagt ggcaacagtt
     5041 cttccacggc aaccgctatt ccgagtccta catggacgcg ctgcccgact tcgtcaagct
     5101 cgccgagggc tacggccacc gcggcgtgct gatcgagaaa cccgctgatg tcgagccggc
     5161 gctgcgcgac gccttcaagc gcaaggacga gctggtgttc ctggacttcc tcaccgacca
     5221 ggccgagaac gtctatccga tggtgccggg cggcaagggc atcacggaaa tgatcctgtc
     5281 cgaagagctt tgatgcgtca catcatctcg atcctggtcg agaacgaggc cggggcgctg
     5341 tcgcgcatct ccaacatgtt ctcggcgcgc ggctacaaca tcgagtcgct gaccgtggcg
     5401 ccgaccgagg acgcctccat gtcgcgcatg accgtggtga ccgtgggctc ggaggacgtc
     5461 atcgagcaga tcaccaagca gctgaacaag ctcgtcgaag tggtcaaggt ggtggacttg
     5521 tcggaagccg agcacatcga gcgcgagatg atgctgatca aggtgcgcgc tggcgccaag
     5581 gagcgcgagg acatgaagcg catggcggat atcttccgcg gccgcatcat cgacgtctcc
     5641 gacaatacct acaccatcga gctgaccggc gagggccgca agctcgacgc gttcatccag
     5701 gcgctcgacc agggcgcgat catcgagacc gtgcgcaccg gcgcgagcgg catcggccgc
     5761 ggcaaccgcg tcctgcgcgt gtaactgcaa gcaagacatg gaaatgggga ccgtccccgt
     5821 tttctgaaac cccgggggaa atgcatgaag gtctattacg acaaggacgc cgacctctcg
     5881 ctcgtcaagg gcaagaaggt caccatcatc ggctacggct cgcagggtca cgcgcacgcg
     5941 ttgaacctcc aggaatcggg cgtcaaggtg accgtcggcc tgagaaaggg cggcgcgtcc
     6001 tgggacaagg cgaagaaagg cggcgtcaag gtcgcggagc tcggcgaggc gatcaagggc
     6061 gccgacatcg tcatgatcct gctgcccgac gagcaccacg cccagatcta cagggactac
     6121 gtcgagccga acatcaagaa gggcgcgtcg cttgccttcg cgcacggctt caacatccac
     6181 tacggcctga tccagccgcg cgccgacctc gacgtgtgga tggtggcgcc gaaggccccc
     6241 gggcacacgg tgcgctccac ctatgcggcg ggcggcggcg tgccgatgct gatcgccgtg
     6301 caccgcaatc cctcgaagaa ggcgcgcgag ttcgcgctct cgtacgccgc ggcgatcggc
     6361 ggcggcaagg ccggcatcat cgagaccagc ttcaaggagg agaccgagac cgatctcttc
     6421 ggcgagcagg cggtgctgtg cggcggctgc gtcgagctgg tcaaggccgg cttcgacacg
     6481 ctggtggacg ccggctacgc gcccgagatg gcgtacttcg agtgcctgca cgagctcaag
     6541 ctgatcgtcg acctgatgta cgagggcggc atcggcacca tgaactactc gatctccaac
     6601 aatgccgagt acggcgaata cgtgagcggc ccgaaagtga tcgatgcggg cacgcgggcg
     6661 cgcatgaagg agatcctcgg gcgcatccag accggcgagt acgcgaagga attcgtgctg
     6721 gagaaccgcg ccggcgcgcc ggagctcctc agccgccggc gcatgaccgc ggagctgccg
     6781 atcgagaagg ttggcgcgcg gctgcgcgag atgatgccct ggatcaggaa gaaccgcctg
     6841 gtcgacacgt cgaagaactg aggagtgaag ccaccgtacc cgcatcccct catcgcgcgc
     6901 gagggctggc cgttcctcgg cgcggcggtg gcggctgcgc tgctcatctg gatcttcgcg
     6961 ggcgggtggt ggtcggtgcc ggtctggctg gccgcgctgt tcgtgctgca gttcttccgc
     7021 gatcccgcgc gcgaggtgcc cgatgacccg cgtgccgtgg tcgcgcctgc cgacgggcgc
     7081 gtggtcgccg tcgagaaagc gcgcgacccc tggctcgagc gcgacgcgct caagatcagc
     7141 gtgttcatga acgtgttcaa cgtgcactcc aaccgcgcgc cggtggacgg cgcggtacgc
     7201 caggtctggt accacgcggg aagcttcgtc aacgccgcgc tcgacaaggc ctcggtcgag
     7261 aacgagcgca acgcgctctg gctgcgcacg gcagacgggg ccgacgtgac ctgcgtgcag
     7321 atcgccggcc tcgtcgcgcg gcgcatcctg tgctacgtcg gcgccggcgc cgagctgcag
     7381 cgcgggcagc gcttcggctt cattcgtttc ggctcgcgcg tcgacgtata cctgcctctc
     7441 gaagcgcagg cggtggcggc gatcggcgac aaggtatacg ctgccgaaac cgtactggcg
     7501 aagctcgccg ggcatgcctg atccgaagga gccgcagcgc caagattacc tgccgggcct
     7561 ggtgccgccg cgcggcggcg cgctgcgccg gcgcggcatc tacctgctgc cgaatctgtt
     7621 cactacggca gcattgttcg cagggttcta cgccatcgtg caggcgatga acgggcgatt
     7681 cggcgagtcc gcgatcgcca tcttcatcgc cgggctgctc gacggcctcg acgggcgcgt
     7741 cgcgcgcctg acgcgtacgc aaagcgagtt cggcgccgaa tacgacagcc tctcggacat
     7801 ggtgtctttc ggcgccgcgc cggcactcgt gctctacgag tgggcgctga aggacctcgg
     7861 gcgcttcggc tggatcgcgg cgttcgtcta ctgcgcgggc gcggcgctgc gcctcgcgcg
     7921 cttcaacgtc atgatcgatg tcgtcgacaa gcgctacttc cagggtctgc cgagcccggc
     7981 ggccgcggcg ctcgtcgccg gcttcgtctg gctcgcgcac gacaacaaga ttccgctcac
     8041 cgagtacggc ctgcagtggg tggcggcgtg cgtcgcgctg tatggcggtc tcaccatggt
     8101 ctcgaacgtc ccgttctaca gcttcaagga cgtcaacttc aggaagagcg tgccgttctg
     8161 ggccatgctg ctcatcgtgc tgggcttcgt gctggtgtcg tcggacccgc cgatcgtgct
     8221 ctttctgctg ttttgcgcct acggcctgtc cggctacgtc atgtgggcgc tcggcaagcg
     8281 acctcggccg cgtccttgac gcccgcgcgc ggcgcgggct atagtgcgcc gcatgcatca
     8341 ggccaagatt ctcggcaccg tcctttcgct cgcgcttctc gcgctgggcg gcctgctgct
     8401 gctgcgcccc gcgcgcgcaa actaacaacc cccaattcgg ctgtacccct gaccgcccac
     8461 ccggcggcaa ggacccatcg cattcccacg aggagcggac catgagcaag ggcaagttga
     8521 tcatcttcga caccaccatg cgcgacggcg agcagagtcc cggcgcgtcg atgaccaagg
     8581 acgagaagct gcgcatcgcg cgcgcgctcg agcgcatgcg cgtggacgtg atcgaagcgg
     8641 gcttccccgc gtcctccaac ggcgatttcg aggcggtgcg cgccatcgcc aacgtggtga
     8701 aggactgcac cgtggccggg ctgtgccgcg cgaacgaccg cgatatccag cgcggcatcg
     8761 acgcgctgag gggcgccaag tcctggcgca tccacacctt catcgccacg agcgagctgc
     8821 atatggcgaa gaagctgcgc atgacgcccg agcaggtgct ggagcagacc aggctctcgg
     8881 tgcgctatgc gaagaacgcc tgcgacgacg tcgagttctc gcccgaggac ggcagccgct
     8941 cggacccgga gttcctgtgc cgggtgctgg aggcggcgat caaggaaggc gccaccacca
     9001 tcaacattcc cgataccgtc ggctacgcgg tgcccgagca gttcggccag atgatccgct
     9061 ccctgcgcga gcgcgtgccg aactcggaca aggcagtgtg gagcgtgcac tgccacaacg
     9121 acctcggcat ggcggtcgcc aactcgcttg ccgcggtggt gatcggcggc gcgcgccaga
     9181 tcgaatgcac catcaacggc ctgggcgagc gcgcgggcaa cacctcgctc gaggaggtgg
     9241 tgatggcggt gcgcacgcgc aaggacttct tcgatctcga gaccggaatc gacaccacgc
     9301 agatcgtgcc gacctcgcgc ctggtgtcgc agatcaccgg cttcgtcgtg cagccgaaca
     9361 aggcggtggt cggggccaac gccttcgcgc acgcctcggg catccaccag gacggcgtgc
     9421 tgaaggcgcg cgaaacctac gagatcatga ccgcggccga cgtcggctgg accaacaaca
     9481 agatcgtgct cggcaagctc tccggccgca atgcattcaa gcagcgcatg aaggagatcg
     9541 gcatcgagct ggagagcgag gacgcctaca acaaggcctt tcagcgcttc aaggacctcg
     9601 ccgacaagaa ggccgagatc ttcgacgagg acctgcaggc gctggtgacg caggaagcgg
     9661 gcaagtcggg cgacgagcac tggcggctgg tgaccatgaa ccaggcgagc ggcatgggcg
     9721 agaaaccgca cgcgcgcatc gtgctggccg agaacggcgc cgagaagaag gccgaatcgc
     9781 agggcgacgg gccggtcgac gcgaccttca aggcgatcga gacggtggcc aagagcggtg
     9841 ccgagctgct gctctactcg gtcaacgcag tcacgcaggg cacggagtcg cagggcgagg
     9901 tgacggtgcg gctctccaag ggtgggcgca tcgtcaacgg cgtcggcgcc gatactgata
     9961 tcgtggtggc ctccgccaag gcctacatca gcgcgctcaa caagctggaa agcaaggtcg
    10021 agcggctcaa cccgcagcat cagctgtgag gcaaccccaa ggagtgagca tcatgagcga
    10081 aggcaagctg aagcgacccg agatcaccgt ggccaccgac gacctcgagc ggctcgaggg
    10141 cctgctgggc gagttgcgcg gcggcgcgcc cgagattgcc gacgggctgc gctcggagct
    10201 ggaccgcgca cgcatcgtcg agccgtccga gattcccccc gacgtggtaa ccatgaactc
    10261 caccgtgcgc ttcaccgacg aggagagcgg caaggaattc cggcgcacgc tgtgctggcc
    10321 ggaggaggtc accgggggcg aggacaaggt gtcgatcctg gcgccgctcg gcagcgcgct
    10381 gctcggcctg tcagtgggcc agcgcatcga ctggccggtg cccggcggac gcatggcgcg
    10441 catccgcatt ctcgacgtgg tgtccaagcc cgggcgcgcg ggcggatagc cgccgtggcg
    10501 aacgcgcgcc gcacatggct gttcgtgccg ggtgcggacg ccgccgcgca tgaagcggcg
    10561 gcgcgttccg gcgccgatgt tctcatccag gagctggaag acttcacgcc gcccgagcgg
    10621 cggccgcagg cgcgccgcct cgccgcgacc ctgtacgagc gctggcgcgc cgccggcgcg
    10681 ctcgccggcg tgcgcgtcaa tccgctcgag acctgcgggc gcgaggacct cgccgcggtc
    10741 ctcgccgggc ggcccgacat cgtgctgatg tcgaaggtgg acgcaccgga acaggtgcgc
    10801 gcgctcgcgg cggcggcgcc gggcgtggag ctggtgccca acatcgaatc cgcgcgcgga
    10861 ttgctgagaa cggccgcaat cgcgcgcgcc gatcgccgcg tcagtgcgct cctggtcgcg
    10921 agcgaggaca tggtggcgga cctcggcacc agccgcagcc gcggcggcga ggagcttgcc
    10981 tatgtgcgcc agcgcttcct gctcgagtgc cgggccgccg gcgtggaagc gatcgactgt
    11041 ccctatacct tcagcgatgc gaagggcgcg gcggcggacg cgcgctgggc gaggcgcatg
    11101 ggctaccgca tgaaaagcct ggtcgacccc tcgcatgcga gggcggtcaa ccgcgtgttc
    11161 accccgaatg tcgccgccgc gcgcaggatc gtggcggcgt tcgagaaggc gcgtgcacag
    11221 ggcaaagaac gggcgcgcgt gaacggcgcg ctgatcgagg tgccgatcta cgctgcggct
    11281 aagcgggtgc tcgagtcagc cgcgcgtacg ccaccgtcga cgcgaagaac gcgaggctga
    11341 gcgcgatctc ggcgctcgcc agccattgcg cggcgccgcg ctcgctccag gcgcgcaccg
    11401 cgccctcggc gaagtaggcg aggatcagca tgctcgacca ctggtaggtg tagcgccggc
    11461 cgttgaggat gccgggaatg gcaagggcga gcggcgcggc tttcagcgcg agcagggagc
    11521 cgccgggccg cagcggcgcc agccacagtt cccacccgag gcacagaagg atgagaaacc
    11581 ccaggctcgc gcacgccgcg agatgggcgg cgcggatcac tgggcgagct tcagcgcgac
    11641 ctgcgccagc cgccggccct gcgcgatgca cagcgcgcgc tcggcatcgg tcaccggctg
    11701 atcgtcggcg atgccggcga agtggctcgg cccgtaaggc gtgccgccgg accgcgtggc
    11761 gttcagttcc gcctgcgtgt agggcgttcc cacgatcacc atgccatggt gcagcagcgg
    11821 caccatcatg gcgagcagcg tcgtttcctg tccgccgtgc atcgaccccg tggaggtgaa
    11881 gacgcacgcg ggcttgccgg cgagcccgcc cttcagccac agcgacccgg tgccgtcgag
    11941 aaaatgcttc agcggcgccg ccatgtcgcc gaagcgcgtc gggctgccca gcgcgaggcc
    12001 cgcgcaggac tcgaggtcgg cgagctcgca gtaaggcgcg ccggactcgg gaatctgggg
    12061 ctgcgcctgg cgcgtcaccg tcgacacgcg gggcacggtg cgcagccgcg cggcggcgcc
    12121 gggcacgcgc tccacgcctt cggccaccag ctccgccagg cgccgcaccg cgccgccgac
    12181 gctgtagtac aggacgagga tgtccgccat ggcctgtatc atacgggccc gatgcgccgc
    12241 cccgatctcc gcgcgctgct cgagttctgc ctcgcgctgg cgcggcgctt ccgcgacgac
    12301 cgcggcatcc agaccgcggg ctcgctcacc ttcaccacgc tgctggccct cgtgccgctg
    12361 gtcacggtcg cgctggcgat gtcgacggcg tttccggtct tcgagcaggc catggaggcg
    12421 ctggggcgct acgtgtcggg gcagctgctg cccgagggcg gcgcccggat cacgcagcag
    12481 ttcgcggcat tcgccgccaa agcaggcagg ctcaccgcgg tcgggctggc gttcctcgcg
    12541 gtgaccgcgc tgatgctgat cctgaccgtg gacgaggtca tgaaccgcat ctttcgcgtg
    12601 cagcgccgcc gcggcctggc gcagcgcctg ctgatgtact gggcggtgct gtcgctcggg
    12661 ccgctgctca tcggcgcgag cctgtccatg acctcgttcc tcgtcggcag ctcgctcggc
    12721 ctgctcaacc tgggatggat gacgcgcggc gtgctcgggc tgatgccgtt cttgttcacc
    12781 tgcgcggcgc tcgcgctgct ctacctcgtg gtgccctacc gcaggatcga ggcgcggcac
    12841 gcgctcgcgg gcgcgatcgt ggccggcatc ctgttcgagt tcgccaagcg cggcttcgcg
    12901 ctctacatcg cgaagttccc gacctactcc ctgatctatg gcgccttcgc caccgtgccg
    12961 atcttcctgc tgtggctcta cctttcctgg gtggtggtgc tgttcggggc gacgctgagc
    13021 gccatgctgc cgggctaccg cggcggctac ggccgccatg ccgatgcgcc cgggcgcgag
    13081 ctgatcgacg ccaccgcggt gttgcgcgtg ctggcgcttg cccggcgcga cgagggcgcg
    13141 ctggcgctcg tcgccctcgc gcggcgcgcg aagctcccgc ccgagcgttg cgagcgggtg
    13201 ctggagcgct gtgccgcgca gggctgggcc gcgcgcaccg accgcgagcg ctggctgctg
    13261 gttcgcgacg ccgataccat tcagctcggt gccgtggtgc gcgcctttgc catggatccc
    13321 gaagcgctgc gcgcaggcgc gccagccgcg ctcgccgagc agctgctgcg cgcggaggac
    13381 tcgctcacca tgacggtgca ggacctgctg caggagcaag acgcatgagc gcgatcgacg
    13441 accgcgggcg cctgatcgcc gcgctcgacg tgccgagcgc cggcgacgcc cgggcgctcg
    13501 ccgagcgcct gggcgacgcg gtgcggttct acaagatcgg cctggagctg ttcagcgccg
    13561 gcggctactt cgagctctcg gactggctgc gcgggcgcgg cgcgcgcgtg ttcgccgacc
    13621 tcaagctcta cgacattccc gaaaccgtgc gtcgcgcggt ggccaacctg cgcgccagcg
    13681 gggcgagctt ccttaccgtg cacgccgagc gctcgatcat ggaggccgcg gcgcgggaga
    13741 agggggacct gaaggtcctc gccgttaccg tgctcaccag cttcgaccag aagagcctcg
    13801 ccgagatggg ctacgccggc agcgtcgagg acctggtgct gcacagagcg cgcgccgcgc
    13861 tcgagtcggg ctgcgacggc gtcatcgcct cggggctgga ggcgcccaag atcaaggcgg
    13921 cgttcggcgg gcgcctgctc gtggtggtgc cgggcgtgcg gccggcgggc gcggcggcgc
    13981 aggaccagaa gcgcgtcgtt gacgtgaagc aggccttcgc cagcggcgcc gactacatcg
    14041 tggtgggccg tccgatccgc gacgcagccg atccgcgcgc cgcggccatc gcgatccagg
    14101 acgcgatcgc gggcgcgttg cagcgctgac gcggtgcaac acgcgggcgc gtaagtcgcg
    14161 tacaatgcgc gactttttgc ttttctgatc aagccgatgc cacgcgccct gcgcaacctc
    14221 gccatcatcg cccacgtcga ccacggcaag acgacgctcg tcgacaagct gctgcgcgcc
    14281 gccggcacct tcgcggccca cgagcgcctc gaggagcgcg tcatggactc caacgacctc
    14341 gagcgcgagc gcggcatcac catcctcgcc aagaattgcg ccgtgcgcta cgaaggcacg
    14401 cacatcaaca tcgtcgacac cccgggacac gccgacttcg gcggcgaggt cgagcgcgtg
    14461 ctgtcgatgg tggacggcgt actgctgctg gtggacgcgg tggaggggcc gatgccgcag
    14521 acgcgcttcg tcacgcgcaa ggcgctcgcg ctcgggctca agccgatcgt cgtcgtcaac
    14581 aaggtggacc gaccgggcgc gcgcgcgcag tggacggtca accagacctt cgacctgttc
    14641 gacaagctgg gcgcaagcga ggagcagctc gatttccccg tcgtctacgc ttcggcgctg
    14701 gaaggctggg ccagcctcga gtccggcgcg cgcggcggcg acatgaagcc gctgttcgag
    14761 gcgatcctcg cgcacgtgcc gatgcgcgac gaggacgccg aggggccgct gcagctgcag
    14821 atctgctcgc tcgactactc gagctacgtc ggcaagatcg gcatcggccg catccggcgc
    14881 gggcgcctca aggcggcaca ggaggtgctg gtgctgaacg gcccggacgc cgcgccgctg
    14941 aaggccaagg tcaaccaggt gcagatgttc gaggggctgc agcgcgtgca ggtcgcccag
    15001 gccgaggccg gggacatcgt gctggtgaac ggcatcgagg agctcggcat cggcaccacg
    15061 ctgtgccatc ccgagcaccc cgaggcgatg gcgccgctca aggtcgacga gccgacgctg
    15121 accatgaact tcctcgtcaa tgcctcgccg ctcgccggcc gcgagggcaa gtacgtgacc
    15181 agccgccaga tccgcgagcg cctggagcgc gagacgaagt cgaacgtggc gctgcgcgtc
    15241 gagtatcccg cggacaccga caccttcatc gtgcacgggc gcggcgagct gcacctgacg
    15301 atcctgctcg agaacatgcg ccgcgaaggc tacgagatcg cggtgtcgcg cccgcgcgtg
    15361 gtctacaagg agatcggcgg ggtgcgctgc gagccgttcg aagtgctcac cgtggacgtg
    15421 gaggaggcca accagggcgg cgtgatggag gagctggggc gccgccgcgg cgagctgctg
    15481 gagatgcatc ccgacggcaa ggggcgcgtg cgcctcgatt accgcatctc ggcgcgcggc
    15541 ctgatcggct tccagggcga gttcatgacg ctcacgcgcg gcaccggcat catgagccac
    15601 gtgttcgacg aatacgcgcc cgcgcggacc gaggagatgg ccgagcgccg caacggcgtg
    15661 ctcgtgtcgc aggacgacgg cgcggcggtc gcctacgcgc tctggaagct gcaggagcgc
    15721 ggccgcatgt tcgtcaaccc cggcgacccg gtgtacgagg gcatgatcat cggcatccat
    15781 agccgcgaca acgacctggt ggtgaacccg atccgcggca agcagctcac caacatccgc
    15841 gcctcgggca aggacgaggc catcgacctc accccgccga tcggcctgac gctggaatac
    15901 gcggtggaat tcatcgccga cgatgagctc gtcgagatca ccccgaagtc gatccggttg
    15961 cgcaagcgct tcctggtgga gtcggagcgc cggcgcgcgg aacgcgcggc ggcctaacca
    16021 gggacctcgc aggcggcgac gccggcttaa acgcgccgcg tacgcaccaa aaaacggtct
    16081 cgccttttct ggcgggatga atctccccgc cagaaaaggc gagagtcttt gaaaggctaa
    16141 acccgagacg agactacggg atcggcaccg cagcgagcag ctgcgcgccg acgtccacgc
    16201 gccgcgcggt gtcgtgcgcg gggcgcaggc ggtgcgcggc gaccccgggc aggatcgtct
    16261 gcacgtcttc cgggatcacc ttgtcgcgct tttccagcag cgcccaggcg cgcgccgagt
    16321 gcaccagcgc gagcgccgcg cgcggcgaca ggcccgccac gtagtccggc gagcgccgcg
    16381 tgtactccac gatcgcctgc acgtaatcga gcagcgccgg cgccacgtgc acgcgctgcg
    16441 cgtcgctctg cagctcgacc agctcggcgg ggctcatgca cgggtcgagg ctcgccagca
    16501 ggtcgcgccg gtcggccccc gcgagcagcg cgcgctcggc gccgcggtcc ggatagccga
    16561 gctcgatgcg catcaggaag cggtcgagct gcgactcggg cagggggaac gtgccaacct
    16621 gctcggaggg gttctgcgtg gcgatgacga agaacggctc ggggagcttc ctcgtctcgg
    16681 tctccaccgt cacctggtgc tcctccatcg cctccagcag cgcgctctgc gtcttgggcg
    16741 tggcgcggtt cacctcgtcc gcgaggatca gctgcgcgaa gatcggaccc ggatggaact
    16801 tgaaggcgcc gctgtcgcgc tcgtacaccg agacgccgat gatgtccgcg ggcagcatgt
    16861 cgctcgtgaa ctggatgcgc tggaaatgca tgccgagcgt cttcgccagc acgtgcgcga
    16921 gcgtggtctt gcccacgccg ggcaggtcct cgatcaacag gtggccgcgc gcgatgaggc
    16981 aggcgagcgc gaggcgcacc tgcgtttcct tgccgaggat cacttcgccg gcggcggcga
    17041 tcaggcgctc gagcggcgcg cgcgtctgcg ccggtttgaa ttgtgttttc agcagcatgg
    17101 cccgattgta cagggtatga tttgaagcac catgaccgtg gcttatgtca cgcacgcgga
    17161 ctgcctgaag cacgagatgg gcgcgtggca tcccgagcgg cccgagcgcc tgacggcgat
    17221 cgaggatcag ctgattgcgt ccggcctcgg gcagtttctg cagcgccatg aggcgccgct
    17281 cgccaccgac gagcagctcg cgcgcgtgca tccgatggag tacgtgcgcg cgatccgcga
    17341 ggccgcgccc accgaaggca cggtgcatct cgaccccgac acggcgatga acccgcacag
    17401 cctgcaggcg gcgctgcgcg cggccggtgc cgtcgtgctc gccaccgacc tcgtgctgcg
    17461 cggggaggtc gatggcgcgt tctgcggggt gcgcccgccg gggcaccatg cgtgccgcgc
    17521 gcggccgatg ggcttctgca tcttcaacaa cgtggccgtg gccgcgcgcc acgcgctcga
    17581 ggcgcacggc ctggagcgcg tcgccatcat cgatttcgac gtccaccacg gcaacggcac
    17641 cgaggacatt ttccagaacg atgcgcgcgt gctgatggcc tccaccttcc agcacccgtt
    17701 ctatccctac agcggcaccg aggacccggc gcccaacatg gtcaacgtgc cgctgcgtgc
    17761 gggcgcgggc tcgaagcagt tccgcgccgc ggtgagcgaa gcctggctgc ccgcgctcga
    17821 gggcttccgc ccacagttgg tgatcttttc cgcggggttc gacgcgcacg ccgatgacga
    17881 catggcgatg ctcagcttca ccgaccagga ctacgcctgg gtgacgcagc aggtgaaggc
    17941 ggtggcggac cggcacgcgg gcggccgcat cgtgtcggcg ctggaaggcg ggtacgccct
    18001 gtcggcgctc ggtcgcagcg tggtgcagca cctgcgcgtg ctcgcggggc tggacgcgta
    18061 acgcagttcc tggtgtaagg tatcggccgt ggggcgcgac tgcgcccgga aggcccccca
    18121 tgcgactgtt caacacgttc acccgattca agaaaggacc ctgatgcgcc tggctcatct
    18181 ggccgttgcc cttgcactgg ctgcgctcgt ccttcccctg catgcgcagt ccctcaaagt
    18241 gtccgcgatc ccggacgaat ctcccaccga gctgctgcgc aagttcaagc cgctgggcgc
    18301 ctacctcgaa aagcaaaccg gcatgaaggt cgactggacg ccggtcaacg actacccggc
    18361 ggtggtcgaa ggtctcgccg ccaagcgcat cgacctcgcc tggctcggcg gcttcacctt
    18421 cgtgcaggcg cgcatccgca ccggcgacgc gatccccatc gcgcagcgcg tggaggacga
    18481 gaagttcatc agctactaca tcgtgccgaa ggcgagcagg gcgaagacgc tgcaggacct
    18541 caagggcaag actttcgcgt tcggctcgcc ggcctccacc tcgggacact tgatgccgcg
    18601 cttcttcctg ctcaaggcgg ggctcaaccc ggacaaggac ttcacgcgcc ttgcgttctc
    18661 gggcgcgcac gacgcgacgg tggcgttcgt cgcctccggc cgcgccgaag ccggcgcgct
    18721 gaacgcttcg gtgtacgaca agctgatcga gaagggcgac gccaacgcaa gatcggtgcg
    18781 catcctgcag accacgccgc cgtactacga ctacaactgg acggtgcgcg gcagcctcga
    18841 cccggcgctg gtgagcaagc tcaccgcggc gttcctcaac ctcgatccga agaacccggc
    18901 gcacaaggag atcctcgatc tgcagcgggc gacgcgcttc gtcgccacca agctcgagaa
    18961 ctacaagggc atcgaggaag cggcgcgctc cgcgggactg ctgaagtagg cacgctgcgc
    19021 acgtgaagct cgacgtcgac gcgctgcacc tggtgctgcc gggcgggcag cgggcgctcg
    19081 cgggcgtttc gctcggcgtg gcgcgcggcg agcgcgtggc cgtcatcggt ccctcggggg
    19141 ccggcaagac cagcctgctg cggatcctgg gcctggcgct gcgcccgagc gccggcaggc
    19201 tcgccctcgc cgacgtcgat ccgtgggtgc tgccggcgcg ggcgctgcag cagttgcgcg
    19261 ggcgcatcgg catgatccac caggcgccgc cgctgccgcc gcgccagcgc ctggtcacgg
    19321 cggtccttgc cggacggctg ggtgcctggg gactgtggcg cgggctcgct tccctcgcgt
    19381 acccggccga tatcgcgggg gcgcgcgacg cgctcggccg gctcgacctc gccgagcgcg
    19441 tcttcgaccg ctgcgaccgg ctctccgggg gccagctgca gcgcgtcggc gtggcacggg
    19501 tgctctacca gaagcccgac ctgatcctcg ccgacgagcc ggtgtcggcg ctcgatccgc
    19561 agctcgccga ccaggccgtg ggcgagctgc accgtgaagc gcaatcgcgc ggcgtgacgc
    19621 tggtggcgag cttgcacgcc gtggacctgg cgctgaagtg gttcccgcgc atcgtcggcc
    19681 tgcgcgacgg cgcggtggtg ttcgactgcc cgccggtgcg cgtgagcgac gccatgctgc
    19741 acgagctgta cgcgaccgaa tcgcagaccc tcccggtcca gggcggcgcg ccgctcgcgc
    19801 atcccgagcc gctgtcgcgc gcgtcgcgcc ggggcgccca gcccctgtgc cgttagcggg
    19861 cgcggccgcg gcaccgcgcg acccggcgct gccggcgcgc gtcgcgctca ccctcgtcgt
    19921 cctgctgttg ctctggccgg cgctcgagct gaccgagttc gacccgggga agctcttcga
    19981 gccgggaaac ctgcagatga tggggcgctt cctcggcacc ttcttcccgc tcgcgctggg
    20041 cgcggatttc ctcgggctgg tgctgcgctc ggccctggag acgctcgcgc tggcgacggc
    20101 cggcatggcg ctcgcggtgg cgctcgctgc gccgctctcg ctcgcggtgt cggagagcct
    20161 ttccgtgtcg cgcatcggtc ccgcgccgct cgccgggctg cgccggctgc tgcgcgcgcc
    20221 gctgcggctg ctgctcgtgg tcatgcgctc gatcccggag atcgtctggg cgctattgtt
    20281 cgtgcgcgcg gtgggcctcg ggccggcggc cggcatcctc gccatcggca tcaactacgc
    20341 cggcatgctc ggcaaggtgt acttcgagat ctacgaggcg ggcgacacgc gcccggcgcg
    20401 cgccgtgctg gaagcgggcg gcggccggct gcacgccttc ttctacggca tcctgccggt
    20461 atcgctgccc gagctggtct cgtacacggt gtaccgctgg gaatgcgcca tccgcgcctc
    20521 cgtggtcatg ggcttcgtcg gcgccggcgg cctcggccag cagatggaac tgtcgatgcg
    20581 catgctggca gggggcgagg tggccaccat gctggtggcg ttcttcctgc tggtgatgct
    20641 cgccgatgcc atcagcgcgc tgctgcgcaa ggtgctgaca tgaccccggg cgccttcgtc
    20701 tggcggccgt gggcggtgct cggcgcgttt gtgctggccg cgctcgccag cctgctgtac
    20761 ctcggcgtcg atttcatcgc gctgttcggg cgtgacagcg tgcgccagat ggcggagttc
    20821 gcgcggggct tcgcgccgcc ggacacctcg cccgagtttc tcggccgcgc ggccgtgggc
    20881 gcggccgaga cgctcgccat ctccgtggtc ggcacgctgc tcgcggcgct cggcggcgcg
    20941 ttcctcgcgc tgccggcctc ggggcgctgg agccgctggc tgcgaccccc ggcgcggctc
    21001 gtgctcaatg tgctgcgctc gattcccgag ctcgtgtggg ccgtgttcat ggtgctcgcg
    21061 gcgggactcg gcccgttcgc cggctgcctc gcgctcgcgg tgcacaccgc gggcgtgctg
    21121 gggcgcctct tcgccgagac gctggagaac gcgccgcccg agccggcgcg cgcgctgcgc
    21181 gacgcgggcg ccggtgcgac gctcgcgttt ctctacggca cgctgcccgt ggcgttgccg
    21241 cagttcacgt cctacgcgct ctaccgctgg gagtacaaca tccgcatggc cgcggtgctc
    21301 gggttcgtcg gcgccggcgg gctcgggcag atgctgtatg tgtcgctgtc ggtgttcaag
    21361 acgcacgagg cggcgacgct gctcctcgcc atgatcgcga tggtgaccct ggtcgacctg
    21421 ctcagcgcct ggctgcgccg cttcctcggc cgctcgggcg cgtgaggccg cgcagcgcgt
    21481 gcaccagcgc ggcgcgctcg gccgcggggg cgaacaggtg acgccgcgcg gcgagcgcgc
    21541 gccgcagccg ccggtagaac tccggctcgc gcgcggcgcg cgccatcagg cgcgcgagcg
    21601 cgcgctcgtc gccgagcggg aagtaccccg ggtacccgcg cccgagcatg ccgatgttcc
    21661 cgggcacgcg ggaagcgagt accggcgtgc cgatgcgcgc ggcctccgcg atcacgttgg
    21721 cgccgccctc catgcgcgag ctcactacca gcaggtggct gcgcgcgagc caggcgagcg
    21781 cgcgcgcgtg cggcaggccg ccgagccagc ggtagcgcgg ctcgccgcgc atgcgccggc
    21841 gcgcttcgcg ctccatcctg gcccgcaagg cagcaccgac gtgcacgact tcgacggcgg
    21901 cgtcccgcgg caggcgcgcg agcgccgcgg cggcacgaaa cggatccttc tcctcgcgca
    21961 ggtggccgat gacggcgacg cgaaatacag cggcgggcgg ctggcgccgc gcgcgcggcg
    22021 cggccgactg aaacacgacg cgcgtcttgc ggcgcagggc cggcgccagg cgccgcagcg
    22081 cttcgtcctg cagcacgatc agccggtcgg cgagcgcaag cgagcggcgt gcatcggccg
    22141 agcccggcag gtcgcggtac aggtcggtgc cggtcagcgc cacggcgagc gcgccgcgcg
    22201 ggtgcgcggc ccggtagcga gccacggcat cgtggctgcg tctcgcgtgc agcgcgagga
    22261 gcagctcgca gggcgcgccg tcccaggcga gcgacaccga cacgcggtgg ccggcgcggc
    22321 gcagaaacgc ggcccagcgc agggcggtgt gccggttgcc gttgcgcgtc ccggcgccgg
    22381 cgggcgtgac cagagcgatg cgcgccatgg gagcggctag actagcacgc catgcttgaa
    22441 cgccagctgc gcgaagcgcg cgcgcgtacc gacctgatga cgcgcgacct cgacggcgag
    22501 cgcctgctcg ggccgatgct cgacatcgtc aatccgccgc tgtgggagat cgggcacgtg
    22561 gcctggttcc aggagcgctg gtgcctgcgc atggcgcaaa gcggcgcgcc catcgattcg
    22621 atcctcgaag gcgccgacgc gctctacgat tccgcggccg tggcgcacga cacgcgctgg
    22681 gacctgccgc tgcccgggcc gcaggcgacg cgcgcctaca tggccgaggt gctcgagcgc
    22741 gtgtgcgcgc ggctgcagcg tgagcccgac aatccggcgc tcgagtatta cgcgcagctc
    22801 tgcctcgccc acgaggacat gcacgccgag gctttccact acacccgcca gacgctcggc
    22861 tatcccgatc cgggcgcggg tgcgccggcg cccgtggcgg ccgcgggcga cgaagcgttc
    22921 gcgggcgggc gcttcgagcg cggcgcgcgg ccgggcgagg gcttcgtgtt cgacaacgag
    22981 aagtgggcgc acgagatcga cgtggcgccg tttcgcatgg cgtgcgcgcc ggtcaccaac
    23041 gccgagtatc tggaatacgt ggagcagggc ggcggggcgc cgccgcgcta ctggaagcgc
    23101 gaagacgggg cctggctgca gcggcgcttc gaccgctggc tgccgctcgc gcccgccggg
    23161 ccggtcctgc acgtgagcgc gctcgaggcc gagggctatt gccgctgggc cgggcgccgc
    23221 ctgccgagcg aagccgaatg ggaattcgcg gcgcgcagcg gccggctggc cgcgggcggc
    23281 gtctgggaat ggaccgcgag cgcgttcctg ccttacccgg gcttttcgcc cgacccgtac
    23341 gaggaatatt cgcggccctg gttcggcacg caccgcgtgc tgcggggcgc gagcttttcc
    23401 actccgccgc gcctcgtgcg ccccgccttc cgcaacttct acatgccgca gcgcgccgac
    23461 gtgttctgcg gcttccggac ctgcgccgtt gcgtagtgct aggcggcttc cttgcccctc
    23521 accgcggcgg gcgtttcctc gacaagcgcg atgacgcgct tggcgtgccg gtagagcttc
    23581 tcgccctttt cggtcaccgc cacgccgcgc cggcggcgct cgaacaactg cgcgtcgagg
    23641 aacagctcga gacgccggat gcgcgccgag acgttggact gcaccgtgtc gagccgcacg
    23701 cccgcccggg caaatccacc ctcttcgtac acggcgacga aatagcgcag atccttgatg
    23761 tccatggccg tagcactata gcaaacgccc tgtgccatct gtatttgagc tagcacaacg
    23821 ggcgccaaac gcaagaaatt gtcagtccac ccgtcagggc acgctcggcc cgacccgctc
    23881 gatgaagtgc gccgactcgg gcgtgacggc ctgctacgcc aggagcacgt cccgggcggg
    23941 cgcgggggcc gccgccagcg cccgggccag cgcgaccagg cccctcatgc gctgagcgtg
    24001 cgcgccaggc cggcgaaaac gtcctcgtcc gccgcggcca cgcgcagcac ccgggcctgc
    24061 ggcaggcgcg cgaaattgcg ctcggccgag cggcgatagg ccgggtcgta gtgctcttcg
    24121 agaagccgcg ccaccagctg cggccaggca ccgcgcgcgg cgagcgcctt ccacgcgtcg
    24181 acgcgctcgc ggccgtgcag cggcgcgagg cactcgagct gcgccgcgag catttcgggc
    24241 gcggcgaaga aatgccggta ctcctccatc agcagcgcga cgcgcgcctc gaggtccgcc
    24301 tcgagccgca cgcactcggc ggcgcgcatg gcgctgatca gcgcctccgg cacctgcagc
    24361 tggccgatct tgcggctctc gccttcgacg tagaccgcgc ggcggcgatc cagcgccgcg
    24421 agcgctgcgt acagccggct ctcgaacatc ttctgcgacg gctgcggctg gtcgggcagc
    24481 tcgcccagta ccgagccgcg gtgcgcggca agcgcctcga ggtccagcac ctgggcgccg
    24541 gcgcgcgcga gcgcgcccag cagccggctc ttgccgctgc cggtggcgcc gtgcaccacg
    24601 cgcagctcga atgccgccgg cagcgcggcg agctgctcga cgatgccgcg gcggtaggct
    24661 cgatagccgc cctcgagcgt ttgcgcgggc cagccgacct cgcgcagcac gtgcgcgaac
    24721 gcggcgctcc gcttgccgcc gcgccagcag tacacgagcg gccgccactg ccgcggccgc
    24781 tcggcgaggg cggtttccag gtggcgcgcg atgttgcgcg cgacgagcgc cgcaccgagc
    24841 cgcttcgccg cgaacggcga tgtctgcgtg tacatcgtgc cgatgcgcgc gcgctccgcg
    24901 tcgtccagca cgggcagcga cagcgcgccc ggaacgtgat cgagcgcaaa ctcggccggc
    24961 gagcgcgcgt cgatgatcgc gtcgaacgcg gtaaggatcg acggctgcat ggcgaccgag
    25021 tgcgatggcg ctttcatcgg ctagaatttt acgccccgtg acgacagaac cggatccgcg
    25081 ccgcgccctc gagcagtacc gccggcacgc gcagggctac gacgcgagcg cgcggcgcac
    25141 gatgcggctg cgccggcgcg ccgtcgactg gctcgagctc gcgccgggcg agcgcgtgct
    25201 cgacgtcgcc tgcggcaccg gcctgtcgtt cccgctgctt gccgagcgcg tcggcgcggc
    25261 cgggcaggtg gtgggcgtcg agctcagccc cgagatgatg cgccaggcgc gcgcgcgcgc
    25321 ggcgccgctg cgcgcgcagc tgcggctcat cgaggcgtcg atggaagacg cggtcctcga
    25381 aggcccgttc gacgccgtgc tgttcaacta cacgcacgac gtgctgcagt cgccgcgcgc
    25441 gctcgccaac atctttgcgc acgtgaagcc cggcgcacgc gtggtggccg cgggggtcaa
    25501 gcatccgccg gcgtggctgt tgccgctgcg gctgctgcgg ctgtggaagg cgcggcccta
    25561 cctcaccacg ttccgcgggc tggaccggcc ctgggcgccg ctcgaggcct atgtcgaagg
    25621 aatggaagtc gagccggtac ttttaggtac gaactacatc gcgcgcgcgc gcgctagaat
    25681 ccacgcgtga acgcgcccga gaaaatccgc ctcaccgagt tcagccacgg cggcggttgc
    25741 ggctgcaaga tcgcgccggc ggtgctgtcc gagatcctcg ccgcgacgcc gatccgcggc
    25801 ctgcccgaag cgctcatggt cggcacggaa acggccgacg atgccgcggt ctaccggctc
    25861 aacgacagcc aggcgctggt ggcgacgacc gacttcttca cgccgatcgt cgacgatccc
    25921 tacgacttcg gccgcatcgc cgcgaccaac gccctctccg acgtctacgc catgggcggc
    25981 cggccgatca tggcgctcgc catcgtcggc atgccgctcg agaagcttcc gctgtcggtg
    26041 atccagagga tcctcgaggg cggggagtcg gtatgcgcct cggtcggcat cccgatcgcg
    26101 ggcggccact cgatcgatac gcccgagccg atctacggcc tggtggcgct cggcctcgtg
    26161 caccccgaca gggtgaagaa gaacagcggc gcgcgcgccg gcgacgtgct ggtgctcggc
    26221 aagccgctcg gcgtaggcat cctttcggcg gtgctgaaga agggcatgat ctcggccgag
    26281 ggctaccggc agatgatcga gtgcaccacg cggctcaaca cgccgggcga ggcgctctcg
    26341 gaaatgccgg gcgtgcacgc gctcaccgac atcaccggct tcggcctcgc cgggcacctg
    26401 ctcgggatct gccgcggctc caagcttgcg gccgaagtga agctcgacgc ggtgccggtg
    26461 atcgcggaag cgctcgactg ggtgaagcag ggcgtcgcca ccggcgcctc cgaccgcaac
    26521 tggaagggct acggcgcgga cgtcgagctg cccgcgggcg cgcccgactg gatgcgcgcg
    26581 ctggtgacgg atccgcagac gagcggcgga ctgctggtcg cgtgcgagcc gcaggccgag
    26641 cgcgcggtgc tgcgggcgtt cgccgatcgc ggcttcaccg aggcgcgcac catcggccgg
    26701 ctgacggccg gcgcgccgcg cgtcagggtc ggctgagcag cggccgcagc gccttccaca
    26761 cgttctcgag caggagcggc tgcgcctctt cggtggggtg gatgcggtca ggctggaaca
    26821 ggcgcagatt gtcggcgatg ccttccagga agaacggcac gaacgcggtc gcatgacgcc
    26881 gcgcgagcgc ctcgaacacg gcggcgaatg cctcggcgta ctccggtccg tagttgggcg
    26941 ggacgcgcat gccggtgaga agcacctttg cgccggcctt cttcgacatg ccgatcatcg
    27001 ccgcgagatt cttctgcatc tcggccgccg gcaggccgcg caggccgtcg ttcgcgccga
    27061 gctcgaggat gacgatcgcg ggcttgtgcc gcgcgagcgc gcgcggcagc cgcgacacgc
    27121 cgccggcggt ggtttcgccg gagatgctcg cgttgacgac gctataatcg agacgctcgc
    27181 gctcgagccg ctcggcgagc agcgtcaccc agccgcgctt ggcggcgatg ccgtaggccg
    27241 ccgacaggct gtcgccgaac accaggatcg tcttgcccca cgcgcccaat gaaaagcaca
    27301 tgaacaagca ggcaacgagc gttctggcgg cgcggggcat cggcaagacg gtgaagagcg
    27361 gcgaggcgga cctcgtcatt ctccgggaaa tcgacctcgc cgtcatgccg ggcgaggtgg
    27421 tggcgatcgt cggcgcctcg ggctcgggga aatcgacgct gctcgcgctg ctcgccggcc
    27481 tggatacgcc caccacgggc acggtcgagc tcgccggcgc ggatctgttc gcgctggacg
    27541 aggacgcgcg cgccgagctg cgcgggcgcg cggtgggctt cgtgttccag tcgttccagc
    27601 tgctgccctc gctcaccgcg ctggagaacg tgatgctgcc gctcgagctc gccgggcgcg
    27661 cggacgcgga ggacaaggcg cgcgagatcc tcgcgcgcgt cgggctcgcc gagcgcctgc
    27721 agcactatcc caagcacctc tcgggcggcg agcagcagcg cgtggcgctg gcgcgcgcct
    27781 tcgtcgtgcg cccgcagctg ctgctcgcgg acgagcccac gggcagcctc gacgtgaaat
    27841 cgggcgaagc ggtgatcgag ctgctgtttc agctcaaccg cgaggccggc accacgctgg
    27901 tcatggtgac gcacgacgag cacctggcgc agcgctgcac gcgcatcgtg cgcctcgccg
    27961 ccggccggat tgccgcctga tgccgctcgc gctgcgcatg ctgggccggg actggcgcgc
    28021 gggcgagctg cgcgtgctcg ccgcggcgct ggtggtggcg gtggcgagca tcaccagcgt
    28081 cgccttcttc gccgaccgcg tgagccgggc gctggtgcgc gacgcgcacc agctgctcgg
    28141 cgccgacctg gtgctggtgt ccgaccatcc gtggaaagcg gggatcggcg aggagatcgc
    28201 gcgccgcggc ctgcagcgcg ccgaagcggt gaatttcatc agcatggcgc gcggcgacgc
    28261 cggcagccag ctcgccggcg tgaaggcggt gacatacaac tatccgctgc gcgggcgcct
    28321 gcgcatcgca gccgcgccca atgcgccgga cgcgccggcc gccacgggcc ccgcgcgcgg
    28381 cagcgtctgg ctcgacgagc ggctggtgag cgcgctcggc gcgccggtgg gcgcgcgcct
    28441 caagctcggc aacgccgagt tcgcggtcgc cgcggtcatc acgctcgagc ccgagcgcgg
    28501 cgccaacttc ttcaatatcg cgccgcgcct catgatgcac gcggcggacg tcccggcaac
    28561 ggggctggtg cagaccggca gccgcgtctg gttctacctc tacgccgccg gcgacccggc
    28621 ggcggtggcg gcgttcgaag cctgggcacg cacgcgcctg gagcgcggcc agcgcgtcga
    28681 aaacctggag agcggccggc ccgaggtgcg cgcctcgatc gagcgcgcgc agcgctttct
    28741 cggcctgacc gcgctcctcg ccgccatcct cgccggcgtg gcgatcgcgc tggccacgcg
    28801 ccgcttcgtc gagcggcacc tggatgcctg cgccgtgatg cgctgcatgg gcgccacgca
    28861 gaagcgcctc ctcggcctgt acggcgcgga gttcctcgtg ctcggcgccg cggcgagcct
    28921 catcggctgc gcgctcggct acctggcgca cggcgcgatc gccgcggcgc tgggcgagct
    28981 gctgcgcgcg cagctcccgc ccgcgtccat cgcgcccgcg ctgcagggct tctcggtcgg
    29041 actggtgctg ctccttggct ttgcgctgcc cccgctcgtg cagctgaaga acgtgcccgc
    29101 ggtgcgcgtc attcgccgcg aggccggcgc gccgcgcggc ggcacggtcg cgatctacgc
    29161 cgcggggctc gccgcgctct ccgcgctgct ggtgtggcag gcgggcgacc tgcggctcgg
    29221 gctctacgtg atcggcggct tcggcgtcgc ggtgctggtc ttcgcggcgg tggccttcgg
    29281 tgcgctgcgc gccgtatcgg cttcggccgt ggcgcggcgc ctgggggcgc gcagctcggc
    29341 gctgcgctac ggcctcgcca acctacggcg gcacgcgcgc ggcaacgcgg tgcaggtggc
    29401 gagcctcgcg ctggggctca cggcggtgct gctgctcacc ttcacgcgca acgacctggt
    29461 ggatgcgtgg cgccggagcg cgccgcccga tgcgcccaac cgcttcctca tcggcgtgca
    29521 gcccgaccag gtcgccgagg tgcgcggctt tctcgccggc gccggcatcg ccgaggcgga
    29581 gctgcatccc atggtgcgcg ggcggctcgc cgccgtgaac ggcaaggcgg tgagcgaagc
    29641 cgactacgcc gaggagcgcg ccaagcgcct ggtcgagcgc gagttcaacc tgtcgtacag
    29701 cgacgagctg ccggggcaca accagatcgc cgcgggccgc tggtacgcgc cgggcgcgaa
    29761 ggagatgtcg gtggaggagg gcatcgccga gcgcctgggc tggaagctcg gcgacgagct
    29821 gaccttcagc gtcggcgccg agcagttcac ggcgcgcatc agctcgctca ggaagctgcg
    29881 ctgggactcg atgaagatca acttcttcgt catcgtgccg ccggcgctgc tggagggcta
    29941 cccggcgagc ttcgtctcgg cgttccgcgt cgcgcccggc gaggagcgcg cgctcgacga
    30001 gctcgcgaag cgctttccca acctcaccct cgtcgacgtg agcgcggcgc tgcgccaggc
    30061 gcagcgcgtg atcgaccagg tgatcaacgc ggtgcagttc gtgttcctgt tcgcgctcgg
    30121 cgcggggctg ctggtgctct actcggcgct ggtggccacc gaggacgagc gccggcgcga
    30181 ggcggcggtg atgcgcgtct acggcgcctc gcgcgcgcac gtcaccggca gccagcgcgt
    30241 cgagttcctc gccatgggac tggtcgccgg gatcctcgcg acgctcggcg cggcggcgat
    30301 cggccagctc ctggcgcggc aggtgttcga gctcgacctg ccgccaagcg cggggctgtg
    30361 gctcgccggg ccgctcgccg gcgtcgtgct gctgtcgctc aacgcttggc tctcggcgcg
    30421 caaggtgctc accgcgtcgc cggcgctgac gctcagggac tcggtctaga atcgcgtatg
    30481 gcagaaatcg ccgcgctcgc cgtcgcgctg gccgccctcg gcgtcgtggt atggcttgcg
    30541 ctgcgcctgc agggtgcggc ggagaagaag caccgcgaaa tgctcaccga cctgcacgac
    30601 gggctcacca ggcagggcga ccgcctgggc gcgcagctcg gcgagctgcg cgaggcggtg
    30661 gcagccaagc tcgatgcgcg cctcgaccag atctcgctgc gggtcaacga gcggctcgac
    30721 gaaggcttca ggaagaccaa cgagaccttc gtcaacgtca tgcagcgcct ggcgaccatc
    30781 gacgaggcgc agaagaagat cgagaccctg accggcagcg tggtgagcct gcaggagctg
    30841 ctgggcgaca agcgctcgcg cggcgcgttc ggcgaggtgc agctcgaggc gctggtcagg
    30901 aacgtgctgc cgacgcaggc cttcgagatg cagtacaccc tgtccaacgg cacgcgcgtg
    30961 gactgcgcgc tgcgcctgcc cgcgcccacc ggcctggtgt gcgtggactc gaagttcccg
    31021 ctggagaact acaagctgat gctcgatccg aaggcgaacg agctcgagcg cgccggcgcg
    31081 caaaaggcgt tccgcgccga cgtgaagcgg cacgtcgacg ccatcgcctc caagtacatc
    31141 atcgagggcg agacctcgga cggcgcggtg atgttcgtgc ccgcggaagc cgtgttcgcc
    31201 gagatccacg cctaccacgc cgaggtggtg gactacgcca tcggcaagcg ggtgtggatc
    31261 gtgtcgccga ccacgctgat ggcggtgctc aataccgcgc gcgccgtgct gaaggacgtg
    31321 gaaacgcgca agcagatcca cgtcatcaag gaagcgctcg cgcgcctggc ggtcgaattc
    31381 ggccggttcg acgagcgcat gaaggagctc gcgcgccaca tccgccaggc ggccgaggac
    31441 gtcgatcgca tccagaccac cggcggcaag atctcgcagc agttccagag aatcgaggcc
    31501 gccgaggtgg acgacgcgcc cgtcgccgag gtggtgcggc tgga
//
LOCUS       DBNHKE010000084        11970 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1390376, whole genome shotgun sequence.
ACCESSION   DBNHKE010000084 DBNHKE010000000
VERSION     DBNHKE010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1390376"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16O9_05315"
     CDS             complement(<1..287)
                     /locus_tag="AC16O9_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6516068.1"
                     /translation="MRQPLEGVTVVALEQVIAGPFCTRQLAELGARVIKIERPGVGDQ
                     SRAMDRTVQGLSSHFVWTNRSKESLALDVKHPLAKPILARLLERADVFVQNL"
     gene            complement(284..1096)
                     /locus_tag="AC16O9_05320"
     CDS             complement(284..1096)
                     /locus_tag="AC16O9_05320"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR6516069.1"
                     /translation="MPESTLKKVSEELIELAASGHTAPPFSARYPKLDAEAGYRAMRR
                     LHQHRLTIGWKPLGRKIGFTNRTLWERYGVHEPIWGFVYDHTLIRSADNRAEVPLENL
                     AQPRIEAEICFGLKAPPPRSHDPHAILAAVEWVAHSVEIVQCFHPDWKVTLPDCTAAN
                     GLHGRLVLGTPVAVESIPHLEALLPAARVVLRKGGKAVDEGVGANVLDSPLYALAHLV
                     EVLALQPDFDQLHAEEIVSTGTLTDAHPVKAGESWSTAFEGLPLPGLTVKFT"
     gene            1194..1589
                     /locus_tag="AC16O9_05325"
     CDS             1194..1589
                     /locus_tag="AC16O9_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6516070.1"
                     /translation="MPITRMDHFNILTDDLPATLAFYEEHLNLKPGARPPFTFPGAWL
                     YADGGRGKDPILHVVAGKDKKVLVKGVIDHMAFYGTGLAETVAKLKAKGIAYELRRLP
                     EYGTWQLFFHDPNNAKVEIDFDAAEAAPA"
     gene            1586..1849
                     /locus_tag="AC16O9_05330"
     CDS             1586..1849
                     /locus_tag="AC16O9_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516071.1"
                     /translation="MNVLQRIVGIALAIVLFVAAFIFASVIFAAAAILALGVWGWLVW
                     KTRDLRKAAAQAKARAEAQGHGTVIEGEYRVERETHRIDNDVQ"
     gene            complement(1850..3811)
                     /locus_tag="AC16O9_05335"
     CDS             complement(1850..3811)
                     /locus_tag="AC16O9_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR6516072.1"
                     /translation="MAAPFHIGGLMAEEKAATSPAAAALRWIALAIAVAMSLYHMYVA
                     GFGPPEALIFRGTHLLFALTLVFILYPFRREGSAAWRALDLLLLVGGWGVVLHIFLNY
                     EYYTNRIIYIDELTAWDKFYAVVSVLVVLDATRRVIGWALPLTAMVFLAYALQSHVAL
                     PVLMEQVYFSTEGIFGQTLGVSASYVMLFVLFGAFMEKSGTGQLFMDFALSITGRYAG
                     GPGKVAVVSSSLFGTVSGSAVANVMVDGPITIPLMKRTGFRPPFAAAVEAVASTGGQL
                     MPPIMGAAAFVMAEFLAVPYAQVALWALVPALLYYLAVFFAVHFEAKRYKLSGVPAAE
                     LPRFGQVMLMRGHLFVPILIVLFGLMAGYSAPLCALVGALACLPLALMRRSTRAGITW
                     KSVIDALEEGARNTLAVAMACACAGIVIACVTITGLGIVFTQLVVALAQDQLILALIL
                     TAMAGIVLGMGMPTTPAYIMMVALLVPAVIKLAVSGCPEPITPECKSSMTPAAHMFAF
                     YFAILSAITPPVALAIFAAAGLAKANMWQSGFAAMRAAAPAYIVPFMFVYEPALLLVS
                     KNWAADWPYVFWVVITACTGVIALAGGLFGWLVGFATTWQRALLIVGALCLIKPGVYT
                     DAIGFGLLALVVGVQLVERRRISESGARA"
     gene            complement(3844..4785)
                     /locus_tag="AC16O9_05340"
     CDS             complement(3844..4785)
                     /locus_tag="AC16O9_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6516073.1"
                     /translation="MKARLSLLALAAGAVIAAPAAAQQVTFMTGPQGGSWIPLGGALK
                     GMWEKNVKGLEITQTPGAGIANVRGVDEGKAHIGLSNSNTAVLGVQGEKPYPKKVTNV
                     CQLANLYPQYFQVVALADANVNSLADLKGKTLVTQPKGNTGELLTEQVLKINGMSYQS
                     LAKANFQAGYTDAVSMMKDGHAQVFTLGTTAPASAVMDLASARDVKLVPVDDKTFNEV
                     KRMNPGHNKLIIKAGTYPKQDKDVPVIGYSTHVIVACDLPEATVYGMVKTMAAGVDDM
                     AAVVKAIAGSTPKDMALDIGVPFHKGAIKFYKEVGAM"
     gene            complement(4886..5824)
                     /locus_tag="AC16O9_05345"
     CDS             complement(4886..5824)
                     /locus_tag="AC16O9_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6516074.1"
                     /translation="MKAEIIATTRIYAPTQKRLDAEFTCHKLHEAADRAAFLKQHAPN
                     VRGLATFGPAGADAALMDALPKLEIISNFGVGVDAIDLAAAKQRKIIVTNTPDVLNDC
                     VADTAMALTVNVMRKFPQAEVYLRAGHWAAKGAYPLTTSLGGKTMGILGLGRIGEDIA
                     KRAQAFGMKIRYHNRNKKDVAYPYDSDAVALARNSDVLMVVTPGGAATDKLVNAQVLD
                     ALGPEGYLVNIARGSVVDEPVLLKYLQEKRIAGAGLDVFADEPKVPPAFFALDNAVLF
                     PHVGSATTETRTAMGNLQVDNLVAHFAGTPVLTRVV"
     gene            complement(5821..6585)
                     /locus_tag="AC16O9_05350"
     CDS             complement(5821..6585)
                     /locus_tag="AC16O9_05350"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR6516075.1"
                     /translation="MELPKNAFKRALRDGNNQIGLWSSLSSNYTVEVIAGAGFDWILL
                     DMEHSPNDLESLLSQLQAAAPYPTHPVVRVPWNDMVTIKRVLDAGAQSLLIPYVQNAS
                     EAKAAVGHTRYPPAGVRGVAGTTRATRFGRIKDYAKRAHEEICVLVQLETKPALDEIE
                     KVAAVDGVDGIFIGPADLHASMGFPGETANSAVLPLIEESVRRIRKAGKAPGYLSPVE
                     ADAKKMLAAGAQFVAVGADVGLLARGAENLLARFKS"
     gene            complement(6603..7649)
                     /locus_tag="AC16O9_05355"
     CDS             complement(6603..7649)
                     /locus_tag="AC16O9_05355"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6516076.1"
                     /translation="MPIARRWSIVAAVPRTAFVTGATGFVGLNLVEELLAQGWRVVAL
                     HRRDSDLTYLRRLPAARVVGDITDAPSVRRAMPQGVDAVFHVAGDTSLWSKRNAAQDR
                     VNVDGTRNVVGAALEKRARRLVQTSSISAYGRHPGRIDESARQLGATSPVNYERSKFL
                     AEEAVRAGLARGLGAVILNPAGILGPYDTRGWGRLFLLVAAGRLPGVPPGSRSFCGVR
                     EVARAHIAAAERGRIGENYLLGGADVSLPELVREICAALGRPAPRRATPAWVLRALGA
                     AGAARGALTGKPPTLTPEAVRMVTGTLTCDSAKAMRELGYRAAPLGEMVRACVAWLAA
                     EGLLPGVESAGRTA"
     gene            7679..8242
                     /locus_tag="AC16O9_05360"
     CDS             7679..8242
                     /locus_tag="AC16O9_05360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6516077.1"
                     /translation="MIVRAWLFLLCSTATFAAEDFHVETTRRGSAVEVRAYAVVAAQY
                     ATVWSTLTDYDAFASFIPGMRSSRVIERKGASWTVEQTGETRFLVFTHPIEVTLLATA
                     QPPHALDVRLLKGNLRRLDGGYRLQPAGRGRIGLLWTGLVEPESLPPLLGELVMRANI
                     KAQFAGMVREIELRQARRDAASPAPPR"
     gene            8239..9672
                     /locus_tag="AC16O9_05365"
     CDS             8239..9672
                     /locus_tag="AC16O9_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516078.1"
                     /translation="MSARWSVLAAPLLAAACATPPTAPPSSECAQWFRALDDQVDAAQ
                     VRDVQEARVPGHPYVRVNRLLASYRGAALRDEAALDALVERMQALDLSARAHEIANLG
                     AAASLERARECGSRLRAADLADARRRAQLLERAAVPDDYVAAYRVLGLYALTRWPFIA
                     GVRDYQAEVRAAFRAGLEPRAGTTVRRYGPPAGPAVPRPALAAMIARASDNPLAIPEP
                     RGAALEALFAAHAPVFEIEIAGTYDQPGALGWRRGEEAPAVDTDAAVVYRMAAWTRYR
                     GRTLLQLVYTIWFSERPAAAPGDLLAGKLDGVTWRVTLAPDGEPLVYDSMHPCGCFHM
                     FFPTPRAAPVPAPHDGMEWMFAPQTLRAVADGERPVLRIATRTHYLERVTLAREPGAA
                     TRYAWRDSDELRSLARPEGGRASAFGPAYGLVAGTGRPERFLFWPMGIRSAGAMRQWG
                     RHATAFVGRRHFDDADLLEKRFVLDLH"
     gene            complement(9686..9811)
                     /locus_tag="AC16O9_05370"
     CDS             complement(9686..9811)
                     /locus_tag="AC16O9_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516079.1"
                     /translation="MQAATSGRARDKVSRHVKQANEGCDFDFLEGTAPTAEPEIH"
     gene            9980..10330
                     /locus_tag="AC16O9_05375"
     CDS             9980..10330
                     /locus_tag="AC16O9_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HIR6516080.1"
                     /translation="MKDIEGLFGKAVGEIERMLNTKTVVGEPITIDGNTLIPLVSVGF
                     GFGVGGGEGTDAKKGAGTGGGTGAGGGVKPVALIVVNKDGVRVETVKGGAASLVEKIA
                     DAAGKFASARPPAG"
     gene            10343..10948
                     /locus_tag="AC16O9_05380"
     CDS             10343..10948
                     /locus_tag="AC16O9_05380"
                     /inference="COORDINATES: protein motif:HMM:NF022615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HIR6516081.1"
                     /translation="MITALAVLAALLVLLVLLLAVPLELAFRCEGLEAFRGEVALGWL
                     FGLVRLRTRLPRPGPSRAAALAPGKRKKRRERNRQAPHGNVMAVLRDAAFRRRVVRLL
                     KDMFAALRVQPFRLYVRLGLGDPADTGRLWAILGPLNALAQNVRNAQLRLEPDFSEPE
                     LRFNAEGRLKIVPLRHLALVIAFALSPASLRAWRTLRGGGA"
     gene            10989..11210
                     /locus_tag="AC16O9_05385"
     CDS             10989..11210
                     /locus_tag="AC16O9_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516082.1"
                     /translation="MRAGTVTLLPVERVVLRSDHGPGRLWVLADKRPHALIVRDESRT
                     WAVGIEGAAVRLETLIEQVQGLDEALASM"
     gene            11356..11625
                     /locus_tag="AC16O9_05390"
     CDS             11356..11625
                     /locus_tag="AC16O9_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516083.1"
                     /translation="MSEPSRTPMSMCPMAETCRGMTQGRRSGLGLWIPGLVFVGLGIL
                     IVIEPRILAWVIAAAFVLLGLMLWMVAGFIRRIGRRFDDTARPGT"
     gene            11755..>11970
                     /locus_tag="AC16O9_05395"
     CDS             11755..>11970
                     /locus_tag="AC16O9_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516084.1"
                     /translation="MNEALLYFASALVAGWGVAHLSATRGVVAGFGELTTDNRRIITM
                     EWVVEGVAFIAVAAFITAATAVDRNAAV"
BASE COUNT         1772 a         4091 c         4240 g         1867 t
ORIGIN      
        1 aggttctgca cgaacacgtc ggcgcgctcg aggagccgcg cgaggatcgg cttcgccagc
       61 gggtgcttca cgtccagggc gaggctttcc ttggagcggt tggtccagac gaaatgcgac
      121 gacagcccct gcacggtgcg gtccatcgcg cgcgactggt cgccgacccc ggggcgctcg
      181 atcttgatga cacgcgcgcc gagctcggcg agctggcgcg tgcagaacgg gccggcgatc
      241 acctgctcga gcgccactac cgtgacgccc tccagcggtt gcctcacgtg aatttcaccg
      301 tgaggccggg aagcggcagc ccctcgaagg cggtgctcca ggactcgccc gccttcaccg
      361 gatgcgcgtc ggtgagcgtg cccgtggaga cgatctcctc ggcgtgcagc tggtcgaagt
      421 cgggctgcag cgccagcacc tcgacgaggt gcgccagtgc atacagcggg ctgtcgagca
      481 cgttcgcgcc gacgccctcg tccacggcct tgccgccctt gcgcagcacc acgcgcgccg
      541 cgggcagcag cgcctccagg tgcggaatgc tctcgaccgc gaccggcgtg ccgaggacga
      601 gccgcccgtg cagcccgttg gcggcggtgc aatcgggcag cgtcaccttc cagtcgggat
      661 ggaagcactg cacgatttcc accgaatgcg cgacccactc gacggcggcg aggatcgcgt
      721 gcgggtcgtg cgagcgcggc ggcggcgcct tcagcccgaa gcagatctcg gcctcgatgc
      781 gcggctgcgc caggttctcg agcggcactt cggcgcggtt gtccgcgctg cggatgagcg
      841 tgtggtcgta gacgaagccc cagatcggct cgtgcacgcc gtagcgctcc cacagcgtgc
      901 ggttggtgaa gccgatcttg cgcccgagcg gcttccagcc gatggtcagg cggtgctggt
      961 gcaggcggcg catggcgcgg tagccggcct cggcgtcgag cttcggatag cgcgcgctga
     1021 acggcggcgc cgtgtggccg ctcgccgcca gctcgatcag ttcttccgat accttcttca
     1081 gtgtggactc gggcatgcgg aaaattctac ccctgcgcgc tggttgcacc ccggcgtttc
     1141 gcgtgccaca ttagcgcgcc atagccagat cgatgcccgc gggggcgctt gcgatgccga
     1201 tcacacgcat ggaccacttc aacatcctca ccgacgacct gccggcgacg ttggcgttct
     1261 acgaggagca cctcaacctc aagcccggcg cgcgcccgcc gttcactttc ccgggcgcgt
     1321 ggctctatgc cgacggcggc cgcggcaagg acccgatcct gcacgtcgtc gccggcaagg
     1381 acaagaaggt cctggtgaag ggcgttatcg accacatggc cttctacggc accgggctcg
     1441 ccgaaacggt ggccaaactg aaggcgaagg gcatcgcgta cgagctgcgc cggctgcccg
     1501 agtacggcac ctggcagctc ttcttccacg accccaacaa cgccaaggtg gagatcgact
     1561 tcgacgcagc ggaggccgca cccgcttgaa cgtgcttcag cgcatcgtcg ggatcgcgct
     1621 cgccatcgtg cttttcgttg cggcgttcat tttcgcgtcc gtgatcttcg ccgccgccgc
     1681 gatcctcgcc ctgggggtat ggggatggtt ggtgtggaag acgcgcgacc tgcgcaaggc
     1741 cgcggcgcag gccaaggcgc gggccgaggc ccaaggccac ggcacggtga tcgagggcga
     1801 gtaccgggtc gagcgcgaga cgcaccgcat cgacaacgac gtgcagtagc taggcgcgag
     1861 cgccgctttc cgagattcgc cggcgctcta cgagctgcac gccgaccacc agcgccagca
     1921 gcccgaagcc gatcgcgtcg gtgtagacgc cgggcttgat caggcacagc gcgccgacga
     1981 tcagcaacgc gcgctgccac gtcgtggcga agccgaccag ccagccgaac aggccaccgg
     2041 caagggcaat gacgcccgtg caggccgtga tcaccaccca gaacacgtag ggccagtccg
     2101 cggcccagtt cttggacacc agcagcagcg ccggctcgta gacgaacatg aacggcacga
     2161 tgtaggcggg cgcagccgcg cgcatggcgg cgaatcccga ctgccacata ttcgccttgg
     2221 ccaggcccgc tgcggcgaag atcgccaggg ccaccggcgg tgtgatcgcc gacaggatgg
     2281 cgaaatagaa cgcgaacatg tgcgccgccg gcgtcatcga gctcttgcat tccggcgtga
     2341 tcggctcggg gcagccggat acggcgagct tgatcaccgc gggcacgagc agcgccacca
     2401 tcatgatgta cgcgggggtg gtcggcatgc ccatgcccag cacgatcccg gccatcgccg
     2461 tgaggatgag cgcgaggatc agctggtcct gggcgagcgc gacgaccagc tgcgtaaaga
     2521 cgatgcccag gccggtgatg gtgacgcagg cgatgacgat accggcgcag gcgcaggcca
     2581 tggcaaccgc gagcgtattg cgggccccct cttccaacgc atcgatgacg gacttccagg
     2641 tgatgccggc acgcgtcgag cgtcgcatca gggcgagcgg caggcaggcg agcgcgccga
     2701 caagcgcaca cagcggcgcc gagtagccgg ccatcaaacc gaacaggaca atgaggatcg
     2761 gcacgaagag gtgtccgcgc atcagcatga cctgcccaaa gcgcggcagc tcggcggctg
     2821 gaacgcccga gagcttgtag cgcttggcct cgaaatgcac cgcgaagaac accgccaggt
     2881 agtacagcag ggccggcacc agcgcccaca gcgctacctg cgcgtatggc acggcgagaa
     2941 actccgccat gacgaaggcc gccgccccca tgatgggcgg catcagctgc ccgccggtgg
     3001 aggcgacggc ttccactgcg gcggcgaagg gcggtcggaa gccggtgcgc ttcatgagcg
     3061 gaatggtgat cgggccgtcc accatgacgt tggccaccgc gctgcccgag acggtgccga
     3121 agaggctcga gctcaccact gcgaccttgc ccgggccgcc ggcgtagcgc ccggtgatgg
     3181 acagcgcgaa gtccatgaac agctgcccgg taccgctctt ttccatgaag gcgccgaaca
     3241 gtacgaacag catcacgtac gaggccgaca cgcccagcgt ctggccaaag atgccctcgg
     3301 tggagaagta cacctgctcc atcagcaccg gcagcgccac gtgcgactgc agcgcgtagg
     3361 cgaggaacac catcgccgtg agcggcagcg cccagccgat gacgcggcgc gtcgcgtcaa
     3421 gcacgacgag caccgagacc acggcataga acttgtccca cgccgtgagc tcgtcgatgt
     3481 agatgatgcg gttggtgtag tactcgtagt tgaggaagat gtgcagcacc acgccccagc
     3541 cgccgacgag cagcagcagg tcgagcgctc gccaggcggc gcttccctca cggcggaacg
     3601 gatagaggat gaagaccagg gtgagggcga acagcaggtg cgtaccgcgg aagatgagtg
     3661 cctcgggtgg cccgaagccg gccacgtaca tgtggtacag cgacatcgcc acggcgatgg
     3721 cgagcgcaat ccagcgcaac gccgctgccg cgggtgaggt cgccgctttt tcctcggcca
     3781 tgagcccccc gatgtgaaac ggggcggcca tgccgccccg ctgtcgttac gccctggcgg
     3841 gcgttacatc gcccccactt ccttgtagaa cttgattgcg cccttgtgga acggcacgcc
     3901 gatgtcgagc gccatgtcct tgggcgtcga gccggcgatg gccttgacca cggccgccat
     3961 gtcatccacg ccggcagcca tggtcttgac catgccgtac accgtggcct cgggcaggtc
     4021 gcaggcgacg atgacgtgcg tcgagtagcc gatcaccggc acgtccttgt cctgcttcgg
     4081 ataggtgccg gccttgatga tcagcttgtt gtgaccgggg ttcatccgct tcacttcgtt
     4141 gaaggtcttg tcgtccacgg gcaccagctt cacgtcgcgc gcgctggcga ggtccatcac
     4201 cgccgaggcc ggcgccgtgg tgccgagcgt gaacacctgc gcgtggccgt ccttcatcat
     4261 cgacacggcg tcggtgtagc cggcctggaa gttggccttg gcgagcgact ggtagctcat
     4321 gccgttgatc ttcagcacct gctcggtgag cagttcgccc gtgttgccct tgggctgcgt
     4381 caccagcgtc ttgcccttca gatccgccag gctgttgacg ttggcatcgg cgagcgcgac
     4441 tacctggaag tactgcgggt agagattcgc cagctggcag acgttggtga ccttcttcgg
     4501 atagggtttc tcgccctgca cgccgagcac cgcggtgttc gagttcgaca gtccgatgtg
     4561 cgccttgccc tcgtccacgc cgcgcacgtt ggcgatcccg gcgccagggg tttgcgtgat
     4621 ctcgaggccc ttcacgttct tctcccacat gcccttcagc gcgccgccga gcgggatcca
     4681 cgagccgccc tgcgggccgg tcatgaaggt gacttgctgg gccgccgcgg gtgcggcgat
     4741 tactgcgccc gccgccaggg cgagcaaaga caatctggct ttcatcctct cctccttcgt
     4801 tgaactgctt gaaatgtcct ggtgcgcgcg ggaatgatag cgcgagtccc gcgcccaccg
     4861 gtgctgcaac gcaataaagc gggcgtcaga cgacgcgagt gagcaccggc gtgccggcga
     4921 aatgggcgac gaggttgtcg acctgcagat tccccatcgc ggtgcgcgtc tcggtcgtcg
     4981 cgctgcccac atgcgggaac agcacggcgt tgtccagggc gaagaacgcc ggcggcacct
     5041 tgggctcgtc ggcgaacacg tccagccccg ctccggcgat tctcttttcc tgcaggtact
     5101 tcagcagcac gggctcatcc accaccgagc cgcgcgcgat gttcaccagg tagccctcgg
     5161 ggccgagcgc atccagcact tgcgcattca ccagcttgtc cgtggccgcg ccgcccgggg
     5221 tgacgaccat cagcacgtcg gagttcctcg ccagcgccac ggcgtcgctg tcgtaggggt
     5281 aggcgacgtc cttcttgttg cggttgtggt agcggatctt catgccgaag gcctgcgcgc
     5341 gcttggcgat gtcttcgccg atgcgcccga ggccgaggat gcccatggtc ttgccgccaa
     5401 gcgacgtggt cagggggtag gcgcccttgg ccgcccagtg cccggcgcgc agatagacct
     5461 cggcctgcgg gaacttgcgc atcacgttca cggtgagcgc catcgccgtg tcggcgacgc
     5521 agtcgttcag cacgtcgggg gtgttggtga cgatgatctt gcgttgcttg gccgcggcga
     5581 ggtcgatggc gtccacgccc acgccgaagt tggagatgat ctccagcttg ggcagtgcgt
     5641 ccatcagcgc cgcgtccgcg cccgccgggc cgaaggtggc cagcccgcgc acgttgggcg
     5701 cgtgctgctt caggaacgcg gcgcggtcgg cggcctcgtg cagcttgtgg caggtgaact
     5761 ccgcatccag gcgcttttgc gtcggcgcgt agatgcgggt ggtggcgatg atctcggctt
     5821 tcatgacttg aaccttgcaa gtaggttttc ggcgccgcgc gcgagcaggc cgacgtcggc
     5881 gcccacggcg acgaactgcg cgccggcggc gagcatcttc ttcgcgtcgg cctccacggg
     5941 cgacaggtac cctggagctt tccccgcttt ccgaatgcgc cgcacgctct cctcgatcag
     6001 cggcagcacc gcggaattgg cggtctcgcc cggaaagccc atcgaagcgt gcaggtcggc
     6061 cgggccgatg aagatgccgt cgacgccgtc aacggcggcc accttctcga tttcgtcgag
     6121 cgcgggcttc gtttccagct gcaccagcac gcagatctcc tcgtgcgcgc gcttcgcgta
     6181 gtccttgatg cgcccaaagc gcgtggcgcg cgtggtgccg gccacgccgc gcacgccggc
     6241 gggcggatag cgcgtgtgcc ccacagcggc cttcgcttcc gacgcgttct gcacgtaggg
     6301 gatgaggagc gactgcgcgc cggcatcgag cacgcgcttg atggtcacca tgtcgttcca
     6361 gggcacgcgc accaccgggt gcgtggggta gggcgccgcc gcctgcagct gcgagagcag
     6421 gctctccaaa tcattcggcg agtgctccat gtcgagcagg atccagtcga agcccgcgcc
     6481 ggcgatcacc tccaccgtgt agttggagga caggctcgac cacaggccga tctggttgtt
     6541 tccgtcgcgc agggcgcgct tgaacgcatt cttcggcagt tccatgggag aaggcggggt
     6601 ggttacgcag tgcggcccgc cgattctacc ccgggcagca ggccttccgc ggcgagccag
     6661 gcgacgcagg cgcgcaccat ttcaccgagc ggcgcggcgc gatagccaag ctcgcgcatc
     6721 gctttggcgc tgtcgcaggt cagcgtgccc gtgaccatgc gcaccgcttc aggcgtgagc
     6781 gtgggcggct ttcccgtcag cgcaccgcgc gcggcgccgg cggcgcccag cgcacgcagc
     6841 acccaggcgg gcgtcgcgcg ccgcggcgcc ggacggccaa gcgcggcgca gatctcgcgc
     6901 accagctctg ggaggctcac gtccgcgccg ccgagcaggt agttttcgcc gatgcgcccg
     6961 cgctcggcgg cggcgatatg cgcccgcgcc acttcgcgca cgccgcagaa gctgcgtgat
     7021 cccggcggca cgccgggcag ccgccccgca gccaccagca ggaacagtcg gccccagccg
     7081 cgcgtgtcgt atggtccgag gatgcccgcc gggttgagga tcaccgcgcc cagaccgcgc
     7141 gccaggccgg cgcgcaccgc ctcctcggcc agaaacttcg agcgctcgta gttgacgggc
     7201 gaggtcgcgc cgagctgccg ggcgctttcg tcgatgcggc cggggtgccg gccgtacgcc
     7261 gagatgctcg aggtctgcac caggcgccgc gcgcgcttct ccagcgccgc gccgacgacg
     7321 tttcgcgtgc cgtcgacgtt gacgcgatcc tgggcggcgt tgcgcttcga ccacaggctc
     7381 gtatcgccgg cgacgtgaaa caccgcgtcc acgccctgcg gcatcgcgcg ccgcaccgac
     7441 ggcgcgtcgg tgatgtcgcc caccacgcgg gcggccggca agcgccgcag gtacgtgagg
     7501 tccgaatccc tgcgatgcag cgccaccacg cgccagccct gcgcgaggag ctcctcgacg
     7561 aggttgaggc caacgaatcc ggtggcgccg gtgacgaacg cggtcctggg cacggccgcc
     7621 acgatggacc agcgccgcgc gatcggcaag tggtgcgatg gctggtacag tgccccggat
     7681 gatcgtgcgg gcgtggctct ttcttctgtg cagcacggcc acgttcgcgg ccgaggactt
     7741 tcacgtcgag accacgcgcc gcggcagcgc agtcgaggta cgtgcctacg cggtggtcgc
     7801 ggcgcagtac gctacggtct ggagcaccct cacggactat gacgcgttcg cctccttcat
     7861 cccggggatg cgcagcagcc gcgtgatcga acgcaagggc gcgagctgga ccgtggagca
     7921 gaccggcgag acgcgcttcc tggtcttcac gcacccgatc gaggtcacgc tgctcgccac
     7981 cgcgcagccg cctcatgccc tcgacgtgcg cctcttgaaa ggcaacctgc ggcgcctgga
     8041 cggcggctac cgcctgcagc ccgcgggccg cggcaggatc gggctgctct ggacgggcct
     8101 ggtcgagccg gaatcgctgc cgccgctgct tggcgaactg gtgatgcgcg cgaacatcaa
     8161 agcgcagttc gccggcatgg tgcgcgagat cgagctgcgg caggcgcgcc gcgacgcggc
     8221 gtcgccggcc ccgccgcgat gagcgcgcgc tggagcgtgc tggccgcgcc gctgctggcg
     8281 gccgcctgcg cgacgccgcc gacggcgccg ccgtcaagcg aatgcgcgca atggttccgc
     8341 gccctggacg atcaggttga cgcggcgcag gtccgcgacg tgcaggaggc gcgcgtcccg
     8401 ggccatccct acgtgcgcgt gaatcggctg ctggcctcgt atcggggcgc cgccttgcgc
     8461 gatgaggccg cgctggacgc gctcgtggag cgcatgcagg cgctcgacct gtcggcgcgg
     8521 gcccacgaga tcgcgaatct cggggctgcg gcgtcgctcg agcgcgcgcg cgagtgcggc
     8581 tcgcgcctgc gcgccgcgga cctcgccgat gcgcgccggc gcgcgcagct cctcgagcgc
     8641 gcggcggtgc ccgacgacta cgtcgccgcg tatcgcgtgc tcggactcta cgcgctcacc
     8701 cgatggccgt tcattgccgg ggtgcgcgac taccaggcag aggtgcgcgc cgcgttccgc
     8761 gccgggctcg agccgcgggc gggaaccacg gtgcggcgct acggcccgcc cgcggggccc
     8821 gcggtgccgc ggcccgcgct ggcggcgatg atcgctcgcg cctcggacaa cccgctcgcc
     8881 atccccgagc cacggggcgc cgcgctcgag gcgctgtttg cggcgcacgc gccggtgttc
     8941 gagatcgaaa ttgcgggcac gtacgaccag ccgggcgcgc tcggctggcg ccggggcgaa
     9001 gaagcgcccg cggtggacac ggacgcggcg gtcgtctacc gcatggcggc atggacccgc
     9061 taccgcggcc gcacgctgct gcagctcgtc tatacgatct ggttctccga gcggccggcc
     9121 gcagcgcccg gcgacttgct tgcgggcaag ctcgatggcg tgacctggcg cgtgacgctc
     9181 gcgccggatg gcgagccgct ggtgtacgac tcgatgcatc cgtgcggctg cttccacatg
     9241 ttctttccca cgccgcgcgc agcgccggtg ccggcgccgc acgacgggat ggagtggatg
     9301 ttcgcgccgc agacgctgcg cgcggtcgcc gacggcgagc gcccggtgct gcgcatcgca
     9361 acgcgcacgc actacctgga gcgggtcacc ctggcgcgcg agccgggcgc ggccacgcgc
     9421 tacgcctggc gcgattccga cgagctgagg tcgcttgcgc ggcccgaggg cgggcgcgcg
     9481 agcgccttcg gcccggcgta cggcctcgtc gcgggcaccg ggcgcccgga gcggtttctg
     9541 ttctggccga tgggcatccg gtcggcgggc gcgatgcggc agtgggggcg ccatgccacg
     9601 gcattcgtcg gccggcgcca tttcgacgac gccgacctgc tggagaaacg cttcgtgctg
     9661 gatctgcact gaagcgcgcg gatcctcagt ggatctcggg ctccgccgtg ggcgcggtgc
     9721 cctcgaggaa gtcgaagtcg cagccctcgt tcgcctgctt cacgtggcgc gagaccttgt
     9781 cgcgcgccct gccgctcgtg gccgcctgca tggaccgcag gaaccgcggg aaggcggcct
     9841 gaggccgttc cttttcgccg ccgccggcgc acactgtctt tgacgtgcct caacgcgccg
     9901 ccgcggtaat gcgctagctt tggttccgtc gcccgggtcg cgcgccgtcg cgttgatcgg
     9961 tcggcatctg gaggcactca tgaaggatat tgaaggcctg ttcggcaagg ccgtcggcga
    10021 aatcgagcgc atgctgaata ccaagaccgt ggtcggcgag ccgattacga tcgacggcaa
    10081 cacgctcatt ccgctcgtca gcgtcggctt cggcttcggc gtgggcgggg gagagggcac
    10141 ggacgcgaag aagggcgccg ggaccggtgg tggcacgggc gcggggggcg gcgtcaagcc
    10201 cgtcgcgctg atcgtcgtca acaaggacgg cgtgcgggtg gagacggtga agggcggcgc
    10261 ggcctcgctg gtggagaaaa tcgccgacgc cgccggcaag ttcgcttcgg ccaggccgcc
    10321 cgccggctag gcggcagccg ccgtgatcac ggcgctcgcg gtcctcgcgg cgctgctcgt
    10381 gctgctcgtg ctgctgctcg cggtgccgct cgagctggca tttcgctgcg aagggctcga
    10441 ggcgtttcgc ggcgaggtcg cgctcggctg gctgttcggg ctggtgcgcc tgcgcacgcg
    10501 cctgccgcga cccggcccgt cgcgcgctgc ggcccttgcg ccgggaaagc gcaagaagcg
    10561 gcgcgagcga aacaggcagg caccccacgg gaatgtcatg gcagtgcttc gcgacgcggc
    10621 atttcgccgg cgcgtcgtcc gcctgctcaa ggacatgttc gccgcgcttc gcgtgcagcc
    10681 gtttcgcctt tacgtgcgcc tgggcctggg cgatccggcg gacaccggcc ggctgtgggc
    10741 gattctcggg ccgctgaacg cgctggcgca gaatgtgcgc aatgcgcagt tgcgcctcga
    10801 gccggatttc tccgagcccg agctccggtt caacgccgaa ggccgcctga agatcgttcc
    10861 gctgcggcac ctcgcgctcg tgatcgcctt tgcgctctcg cccgcgtcgc tgcgcgcctg
    10921 gcgcacgctg cggggcggcg gtgcctgacg gcgcgagcca tgggcagggg cttcgcgccg
    10981 ggacgccggt gcgcgcggga accgtcacgc tgctgcccgt cgagcgcgtc gtgctgcgct
    11041 cggaccatgg tccggggcgc ctgtgggtcc tggcggacaa gcgtccccat gcgctcatcg
    11101 tgagggatga gtcgcgtacc tgggccgtcg gaatcgaggg cgccgcggtg aggctcgaga
    11161 cgctgatcga gcaagtgcag gggctcgacg aagcgctggc gtcgatgtga gcccggcggt
    11221 cgagcgccgc gcctcggggg cgaatccacc gatctttgag gttcctcaac accccgaacc
    11281 ggggaagtgt tatgcgtgaa gctgcgcgtg gccgaagctc ggggcgccgc gcgagccccg
    11341 gcaatggagg cgaacatgag cgaaccttca cgcaccccga tgtcgatgtg cccgatggcc
    11401 gaaacctgca ggggcatgac gcaggggcgg cgctcgggac tgggcctctg gattcccggg
    11461 ctggtcttcg tggggcttgg catcctgatc gtgatcgagc cgcgcatcct cgcctgggtc
    11521 atcgccgccg cattcgtcct gctcggcctc atgctgtgga tggtggcggg attcattcgc
    11581 cggatcggcc ggcgcttcga cgacacggcg cggcccggca catgagcgcc gaggggctgt
    11641 gaccgctcgc gcgcttcatt ctcatcggct gcgggcgggt ggtgctatgg ggggcattcc
    11701 ggggaaagca ccggaacgaa aagtaggagc cggcgaacca ggcgcgacgc cgccgtgaac
    11761 gaggcgctgc tttacttcgc gtcggcgctc gtcgcgggct ggggcgtcgc gcacctatcg
    11821 gccacgcgcg gcgtggtcgc gggattcggc gagctgacca ccgacaaccg gcgcatcatc
    11881 accatggagt gggtggtcga gggcgtggcg ttcatcgccg tcgccgcgtt catcacggcg
    11941 gccaccgcgg tcgaccggaa tgccgcggtc
//
LOCUS       DBNHKE010000085         8782 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1178433, whole genome shotgun sequence.
ACCESSION   DBNHKE010000085 DBNHKE010000000
VERSION     DBNHKE010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1178433"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16O9_05400"
     CDS             complement(<1..219)
                     /locus_tag="AC16O9_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HIR6516085.1"
                     /translation="MSASALAAVGVGAMLGAWARWGLAGWLNHLTPSLPLGTLVANVA
                     GGYAVGLALAFFAAHPSRAPEWRLAGITG"
     gene            complement(216..629)
                     /locus_tag="AC16O9_05405"
     CDS             complement(216..629)
                     /locus_tag="AC16O9_05405"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR6516086.1"
                     /translation="MKASVQREDFDAGAEIRALSSDPRVGAVASFVGVVREVGMTLEH
                     YPGMTEKALHRIVDEATGRWQVLGCTVIHRYGELKPTDQIVLVAVASAHRGDAFAACE
                     FIMDYLKTQAPFWKKEAAGWVEARAADDKAAARWK"
     gene            complement(626..883)
                     /gene="moaD"
                     /locus_tag="AC16O9_05410"
     CDS             complement(626..883)
                     /gene="moaD"
                     /locus_tag="AC16O9_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HIR6516087.1"
                     /translation="MKVRVLYFAALREQLGTPGEEIELPAGVGTVAALRSHLRARGGA
                     WELALAEGKLVRAALNQDMAPPTSALKAGDEVAFFPPVTGG"
     gene            complement(880..2085)
                     /locus_tag="AC16O9_05415"
     CDS             complement(880..2085)
                     /locus_tag="AC16O9_05415"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR6516088.1"
                     /translation="MNKGLLSVDEALEVLLAGARPVAEVQEVPTLEATGRVLARSQAS
                     TMDVPPMDNSAMDGYAVRTADVKAPDTKLKVAQRIMAGAVGQALAPGTAARIFTGAPI
                     PPGADAVVMQEFCAADGEAVTIKKVPQKDAWIRRRASDIAKGAQVLTAGIRLRPQDTG
                     LAASVGIKTLPLYRRVRMGLFFTGDELVMPGDPLPPGRIYNSNRFTLNGLAATFGCEV
                     RDYGIVPDTLEDTRAVLKRAAAECDVIVTSGGVSVGDADFVKPAVEAEGSLLMWKIAM
                     KPGRPLAFGKVGDAAFIGLPGNPVSSFVTFLIFVRPFLLKTQGIAAVQPRTVAARADF
                     DWPEPDARREYLRVKWNAQGGLEIYPTQDSAVMTSTAWADGLVDNPPNHAIRRGDTVR
                     FLPYAELYG"
     gene            complement(2082..2576)
                     /gene="mobB"
                     /locus_tag="AC16O9_05420"
     CDS             complement(2082..2576)
                     /gene="mobB"
                     /locus_tag="AC16O9_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR6516089.1"
                     /translation="MKTFGFAGWSGSGKTTLIEKLIPRFTARGLRVSLIKHAHHTFDV
                     DHPGKDSYRHRYAGASEVLVTSSRRWVLMHELRGAQEPSFEDQVRHLSPCDLLIVEGF
                     KHAPIPKLEVWRALTGEGLLHPQDPHIVAVASDTKLETKLPLLDLNDDAGIAEFIVRH
                     LKLA"
     gene            complement(2573..3328)
                     /locus_tag="AC16O9_05425"
     CDS             complement(2573..3328)
                     /locus_tag="AC16O9_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6516090.1"
                     /translation="MIGASDPLIALLAAGMLAAAFVYASVGHGGASAYIAAMALAGIA
                     PAEMRPVALTLNVIVSSLATWKFWRAGYFRWRLFWPFAAVSIPFAYLGGAIALPGNVY
                     KVVVGAVLVYAAWPLWRSAAAGAEMRPVREPAVPLALAIGAALGLLAGLTGVGGGIFL
                     SPLVLLLGWAGTKQTSAVAAPFILVNSLAGLSAGYVAGTAALPPAIWLLAAAVLVGGW
                     LGAEYGSRRFTNPIVRRLLAAVLVLAGAKLILT"
     gene            complement(3353..4156)
                     /locus_tag="AC16O9_05430"
     CDS             complement(3353..4156)
                     /locus_tag="AC16O9_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516091.1"
                     /translation="MGLRAILLVALLALAPAARAQSTFIVVSSTTSTENSGLFKHLLP
                     IFRNKTGIEVRVVAQGTGQALDTGRRGDCDVVFVHDKAAELRLVEEGWFVGRRDVMYN
                     DYVIVGPRSDPARVGGMQDAVAALKKFQDAKAAFASRGDKSGTHSAELRLWTAAGIEP
                     KGAWYRETGSGMGATLNTASGMNAYALTDRGTWLAFKNRAELAILVQGDARLFNQYGV
                     MLVNPARHPHVKRASGEAFIGWLVSPAGQAAIAAYAIGGEQLYFPNAGR"
     gene            complement(4147..4857)
                     /locus_tag="AC16O9_05435"
     CDS             complement(4147..4857)
                     /locus_tag="AC16O9_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6516092.1"
                     /translation="MNGILPLRLEQVSYVAGGRAIIDGVSLTLEAGASTIILGANGAG
                     KSVLMRLMHGLLAPSAGRIEWRARGASRRQAMVFQRPVMLRRSALANVTYALEAAGVA
                     APERERLAREALAEVGLIAAAARPARVLSGGEQQRLALARAWALHPEVLFLDEPTASL
                     DPGATREIESIIRAFDAAGTKIVMSTHSLGQARRLGDEVVYLHQGRVLERAPVGAFFP
                     RPASPEAAAFVKGELPWA"
     gene            complement(4854..5558)
                     /locus_tag="AC16O9_05440"
     CDS             complement(4854..5558)
                     /locus_tag="AC16O9_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516093.1"
                     /translation="MRRMLEALGAGLRLVATLDLRLWEIVGLSLAVSLSAVALATLAG
                     LPLGAVLAVGRFPGRQALIVVLNALMGLPPVVVGLFIYLLLSRAGPLGELGLLFTPRA
                     MVIAQTVLVLPIIAALARQAVEDAWLEYEEQLRSLGVDGLRGALTVLWDIRFSLLTAV
                     LAGLGRASAEVGAVMIVGGNIDGVTRVMTTAIALETSKGDLPLALGLGMVLIAIVLAL
                     NAGAHLIRDAAARRYG"
     gene            5632..6633
                     /locus_tag="AC16O9_05445"
     CDS             5632..6633
                     /locus_tag="AC16O9_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024138.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6516094.1"
                     /translation="MHTGFTVEFDHAWLAGRELPRERGRALLRLLAALAEAESLREAA
                     RRADLSYRSAWGALADGARLFGAPLVDMQRGRRARLSALGRRVLEADERVRSALGAHF
                     ERLRAEIPALLADALPGAQPRLVVHASHDLALAELAPLCRGTLDLEVVFRGADDCLAA
                     LARGECDVAGFHVADALPRAAAAAAALGRWLDPRKHTLLHFVTREQGLIARPGARIRG
                     LHDLARPGVRFIHRQSRGADTPSVAEAVAAGHADAGFGLRADATRHRLAFTPLATERY
                     FFACARTALRGAALKALLDTLKGSAFAVRVGRLPGYDASKSGRREALDAALTWVGSA"
     gene            6738..7037
                     /locus_tag="AC16O9_05450"
     CDS             6738..7037
                     /locus_tag="AC16O9_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516095.1"
                     /translation="MDASITAAALQQALRSSNPPLVIDVRKRPAFLAAPDMIRGALRR
                     DPESPGALPCADDVVVYCVHGHEMLEHGMVMYDALYAWCRSTKSEVHTWNPALIR"
     gene            7013..8335
                     /gene="chrA"
                     /locus_tag="AC16O9_05455"
     CDS             7013..8335
                     /gene="chrA"
                     /locus_tag="AC16O9_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR6516096.1"
                     /translation="MEPRPDPVSLGEATRFWLKLGFISFGGPAGQIAIMHQELVERRR
                     WISENRFLHALNYCMLLPGPEAQQLATYLGWLMHRTWGGIIAGGLFVLPSVFILIALS
                     WIYLVWGEVPLVAGIFYGIKPAVVAVVAAAAWRIGARTLKNEWHWGIAVAAFLALAVL
                     GVPFPAIVAAAAVIGYAGGRIAPAKFSGNGGHGAGKRGYGPALVDDDTPTPPHALFRW
                     PRFFAILGIAAALWALALGALALAFGWPGTLTQMGWFFTKAAFLTFGGAYAVLPYVYQ
                     AAVEHHQWLTAPQMIDGLALGETTPGPLIMVVAFVGFVGAWAREALGPEALALAGTAG
                     ALVATYFTFLPSFVFIFAGAPFVETTHGNLRFTAPLAGITAAVVGVVVSLAVFFARHV
                     FFTAEAIDWISIVLAAAALAALLRFKAGLIPVILGCGAAGLCYRVLLN"
     gene            8347..8643
                     /locus_tag="AC16O9_05460"
     CDS             8347..8643
                     /locus_tag="AC16O9_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6516097.1"
                     /translation="MAGYVVVEVDVTDSAAYEEYRKLVPATIAKYGGRFLVRGGAIES
                     KEGGWQPKRLVVLEFPSLEDARKWYASPEYAPALALRLKAARSKLLLVEGAPPA"
BASE COUNT         1236 a         3215 c         3054 g         1277 t
ORIGIN      
        1 cccggtgatg cccgccaggc gccattcggg cgccctcgac gggtgggcgg caaagaaggc
       61 cagcgccagc cccaccgcgt agccgcccgc gacgttcgcg acgagggtgc cgagcggcag
      121 gctcggcgtg aggtgattca gccagccggc gagcccccag cgcgcccagg ccccgagcat
      181 ggcacccacg ccgaccgcgg cgagagccga cgcgctcact tccagcgcgc cgccgccttg
      241 tcgtcggcgg cgcgcgcctc gacccagccc gccgcctcct tcttccagaa cggcgcctgc
      301 gtcttcaggt aatccatgat gaactcgcac gcggcgaagg cgtcgccgcg gtgcgcgctc
      361 gccaccgcca ccagcacgat ctggtccgtg ggcttcagct cgccatagcg gtggatcacg
      421 gtgcagccca gcacctgcca gcggcccgtg gcctcgtcga cgattctgtg cagcgccttt
      481 tccgtcatgc ccgggtagtg ctcgagcgtc atgcccacct cgcgcaccac gccgacgaag
      541 ctcgccacgg cgccgaccct gggatcggac gaaagggcgc ggatctcggc gcccgcgtcg
      601 aagtcctcgc gctgcaccga cgccttcatc cgccggtcac cgggggaaag aacgcgacct
      661 cgtcgcccgc tttcagcgcc gacgtgggcg gcgccatgtc ctggttcagc gccgcgcgca
      721 cgagcttgcc ctcggcaagc gcaagctccc aggcgccgcc gcgcgcgcgc aggtgcgacc
      781 ggagcgccgc cacggtgccg acgcccgccg gcagctcgat ctcctcgccc ggggtgccga
      841 gctgctcgcg caacgcggcg aaatagagca ccctcacctt catccgtaga gctccgcgta
      901 gggcaggaag cgcaccgtgt cgccgcgccg gatggcgtgg ttgggcgggt tgtccaccag
      961 gccgtcggcc caggccgtcg aggtcatcac cgccgagtcc tgcgtcggat agatctcgag
     1021 gccaccctgc gcgttccatt ttacgcgcag gtattcgcgc cgcgcgtcgg gctcgggcca
     1081 gtcgaagtcg gcgcgcgcgg ccacggtgcg cggctgcacc gcggcgatgc cctgcgtctt
     1141 gagcaggaac ggccgcacga agatgagaaa ggtcacgaag ctggacaccg ggttcccggg
     1201 caggccgatg aaggcggcat cgcccacctt gccgaaggcg agcgggcgcc cgggcttcat
     1261 cgcgatcttc cacatgagga gcgagccttc cgcctcgacc gcgggcttga cgaagtcggc
     1321 gtccccgacc gagacgccgc ccgaggtgac gatcacgtcg cactcggcgg cggcgcgctt
     1381 tagcaccgcg cgcgtgtcct cgagcgtgtc gggcacgatg ccgtagtcgc gcacctcgca
     1441 gccgaaggtg gcggcgagcc cgttcagcgt gaaacggttg gagttgtaga tgcgcccggg
     1501 cggcagcgga tctcccggca tcaccagctc gtcgccggtg aaaaacaggc ccatgcgcac
     1561 gcgccgatag agcggcagcg tcttgatgcc cacggacgcg gcgagacccg tatcctgcgg
     1621 ccgcagccgg atgccggcgg tcagcacctg cgcacccttc gcgatgtcgc tcgcgcgacg
     1681 ccggatccag gcgtccttct gcggcacctt cttgatggtc acggcctcgc cgtcggcggc
     1741 gcagaactcc tgcatgacga cggcatccgc cccgggcggg atcggcgcac ccgtgaagat
     1801 gcgcgcggcg gtgccgggcg cgagcgcctg gcccaccgcg ccggccatga tgcgctgggc
     1861 caccttcagc ttcgtatcgg gcgccttgac gtcggcggtg cgcaccgcat agccgtccat
     1921 ggcgctgttg tccatcggcg gcacgtccat ggtggaggcc tgcgagcgtg cgagcacgcg
     1981 cccggtggct tcgagcgtgg gcacttcttg cacctcggcg accggccgcg cgccggcgag
     2041 cagcacctcc agcgcctcgt ctacggaaag cagtcccttg ttcatgcgag cttcaggtgc
     2101 ctgacgatga attcggcgat cccggcgtcg tcgttgagat ccagcagcgg cagcttcgtc
     2161 tccagcttcg tgtcgctcgc gacggcgacg atgtgcgggt cctgcgggtg cagcagccct
     2221 tcgccggtga gcgcgcgcca cacttcgagc ttggggatcg gcgcgtgctt gaagccttcc
     2281 acgatcagca ggtcgcaggg cgacaagtgc ctgacctggt cctcgaacga aggttcctgc
     2341 gcgccgcgca gctcgtgcat cagcacccag cgccgcgacg aggtcaccag cacctcggag
     2401 gcgccggcgt agcggtggcg gtaggagtcc ttgcccgggt ggtcgacgtc gaaggtgtgg
     2461 tgcgcgtgct tgatgagcga gacgcgcaag ccccgcgccg tgaagcgcgg gatcagcttt
     2521 tcgatcagcg tggtcttgcc gctgccggac cagccggcga agccgaaggt cttcatgtca
     2581 ggatcagctt cgcgccggcc agcaccagca ccgcggcgag cagccggcgc acgatcgggt
     2641 tggtgaagcg ccggctcccg tactcggcgc cgagccagcc gccgacgagc acggcggccg
     2701 cgagcagcca gatcgccggc ggcagcgcgg cggtgcccgc gacgtagccc gcgctcagcc
     2761 cggcgaggga gttgacgaga atgaagggcg cggcgaccgc ggaggtctgc ttggtccccg
     2821 cccagccgag gaggagcacc agcgggctga ggaagatccc gccacccacg ccggtcagcc
     2881 cggcgaggag ccccagcgcg gcgccgatgg cgagcgcaag tggcaccgcg ggctcgcgca
     2941 ccgggcgcat ctcagcgccc gccgcggcac tgcgccacag cggccaggcg gcgtacacca
     3001 gcaccgcgcc gaccaccacc ttgtagacgt tgcccgggag cgcgatcgcg ccgcccaggt
     3061 aggcgaacgg gatcgacacc gcggcgaacg gccagaacag gcgccagcgg aagtagcccg
     3121 cgcgccagaa cttccaggtg gcgagcgagg acacgatcac gttcagggtg agcgccacgg
     3181 gccgcatctc ggccggggcg atccccgcga gcgccatggc cgcgatgtag gccgacgcgc
     3241 cgccatggcc gaccgaggcg tagacgaagg cggccgccag catgccggcg gcgagcagcg
     3301 caataagggg gtcgctcgca ccgatcacct tcgctattta tcatgaaacg cgtcagcgcc
     3361 cggcgttggg aaagtacagc tgctcgcccc cgatcgcata ggcggcgatc gctgcctgtc
     3421 ctgcgggcga caccagccag ccgatgaacg cctcgccgct cgcccgcttc acgtgcggat
     3481 gcctggccgg attgaccagc atgacgccgt actggttgaa caggcgcgcg tcgccctgca
     3541 ccaggatcgc gagctcggcc cggttcttga acgcgagcca ggtgccgcgg tcggtgagcg
     3601 cataggcatt catgcccgag gccgtgttca gcgtcgcgcc catgcccgag cccgtctcgc
     3661 gataccaggc gcctttcggc tcgatgccgg ccgccgtcca aaggcgcagc tcggccgaat
     3721 gcgtgccgct cttgtcgccg cgcgaggcga aagccgcctt cgcgtcctga aacttcttca
     3781 gcgccgccac cgcgtcctgc atgcccccga cgcgcgcggg atccgatcgg ggcccgacga
     3841 tgacgtagtc gttgtacatg acgtcgcgcc ggccgacgaa ccagccctcc tcgaccagcc
     3901 tgagctcggc ggccttgtcg tgcacgaaga cgacgtcgca gtcgccgcgc ctgccggtgt
     3961 cgagcgcctg gccggtgccc tgggcgacga cgcgaacctc gatgccggtc ttgttccgga
     4021 atatcggcag gaggtgcttg aacaggccgg agttctcggt cgaggtggtc gacgacacga
     4081 cgatgaaggt gctctgcgcc cgtgccgccg gcgccagcgc gagcagcgcg acaagcagga
     4141 ttgccctcag gcccagggca gctctccctt cacgaaagcc gcggcctcgg gcgacgccgg
     4201 ccgggggaag aacgctccca cgggcgcgcg ctcgagcacg cgcccctggt gcaggtagac
     4261 cacctcgtcg cccaggcgcc gcgcctggcc caggctgtgc gtcgacatga cgatcttggt
     4321 gcccgcggcg tcgaaggcgc ggatgatgct ttcgatctct cgcgtggcgc cgggatcgag
     4381 gctggcggtc ggctcatcga ggaacagcac ctcggggtgc agcgcccagg cgcgcgcgag
     4441 cgccaggcgc tgctgctcgc cgcccgagag cacgcgcgcc ggacgcgcgg cggccgcgat
     4501 caggccgacc tcggcgagcg cctcgcgcgc cagccgctcg cgctcgggcg ccgcaacgcc
     4561 cgcggcctca agcgcatagg tcacattggc gagcgccgat cggcgcagca tcaccggccg
     4621 ctggaacacc attgcctggc ggcggcttgc gccccgcgcg cgccactcga ttcggccggc
     4681 gctcggcgca agcagcccgt gcatcagccg catgaggacg ctcttgcccg cgccgttggc
     4741 gcccaggatg atggtgctcg ccccggcctc gagcgtcagg ctgacgccat cgatgatcgc
     4801 gcgcccgccc gcgacgtacg acacctgctc cagccgcagc ggcaggatgc cgttcatccg
     4861 tagcgccgcg cggccgcgtc gcggatcagg tgcgcccccg cgttcagcgc caggacgatc
     4921 gcgatcagca ccatgcccag gccaagggcg agcggcaggt cacccttgga agtctccagc
     4981 gcgatggcgg tggtcatcac ccgcgtcacc ccatcgatgt tgccgccgac gatcatcacc
     5041 gcgccgacct cggcgctcgc acggccgagc cccgcgagca ccgcggtgag cagcgagaag
     5101 cggatgtccc acagcaccgt gagcgcgccg cgcagcccgt cgacgccgag cgagcgcagt
     5161 tgctcttcgt actccagcca tgcgtcctcc acggcctggc gcgcgagcgc ggcgatgatc
     5221 ggcagcacga gtaccgtctg cgcgatgacc atggcgcgcg gcgtgaagag cagccccagc
     5281 tcgccgagcg ggccagcgcg cgagagcagc aggtaaatga agagcccgac caccaccggg
     5341 ggcaggccca tcagcgcgtt cagcaccacg atcagcgcct ggcgaccggg aaaacggccc
     5401 accgccagca cggcgccgag cggcagcccg gcgagcgtgg cgagcgccac ggccgacagg
     5461 ctcaccgcca gcgacaggcc gacgatctcc cacaagcgca ggtcgagggt ggcgaccagg
     5521 cgcagcccgg cacccagcgc ctcaagcatg cgtcttatgc atatgactgc atatcgtcat
     5581 cactcccgtc actttacagg gccgtttcta ggtatttaaa taggcacact catgcataca
     5641 gggttcaccg tcgagttcga ccatgcctgg ctggcggggc gcgagctgcc gcgcgagcgc
     5701 ggccgggcgc tgctcaggct gctcgccgcg ctggccgagg ccgagtcgct gcgcgaggcc
     5761 gcgcggcggg ccgacctgtc ataccgctcg gcctggggcg cgctcgccga cggcgcgcgc
     5821 ctgttcggcg cgccgctcgt cgacatgcag cgcggccggc gcgcgcgcct gagcgccctc
     5881 ggccgccgcg tgctcgaggc cgacgagcgc gtgcgcagtg cgctcggggc gcacttcgag
     5941 cgcctgcgcg cggagatccc ggcgctgctc gccgacgcgc tgccgggggc gcagccgcgc
     6001 ctcgtggtgc atgcgagcca cgatctcgcg ctcgccgagc tggcgccgct gtgccgcggc
     6061 acgctcgatc tcgaggtggt gttccgcggc gccgatgatt gcctggcggc gctcgcgcgc
     6121 ggcgagtgcg acgtggcggg gtttcatgtt gccgatgcgc tgccgcgcgc cgccgcggcg
     6181 gccgccgcgc tggggcgctg gctcgatccg cgcaagcaca cgctgctgca tttcgtcacg
     6241 cgcgagcagg gcctgatcgc gcgaccgggc gcacgcattc gcggcctgca cgacctcgcg
     6301 cgaccgggcg tgcgcttcat ccaccgccag tcgcgcggcg ccgacacacc ctcggtcgcc
     6361 gaagcggtcg ccgcagggca tgccgatgcc ggattcggac tgcgcgccga cgccacgcgt
     6421 caccggctcg ccttcacgcc gcttgccacc gagcgctact tcttcgcctg cgccaggacc
     6481 gcgctgcgcg gcgccgcgct gaaggcgctg ctcgacactt tgaaagggag tgcattcgcg
     6541 gtgcgcgtgg gccggctgcc cggctacgac gcttccaagt ccgggcgccg ggaggcgctc
     6601 gatgccgcgc tcacctgggt cgggtccgcg tgacagggtc ggcggcgcac gcgcatagtc
     6661 ggcgccgcgg ggacttgcag actcccctgc gaagacgccc tcgcgtccaa gctctgcgga
     6721 ctctccagga gagacccatg gacgcgagca tcaccgccgc tgcactccag caagcgcttc
     6781 gttcaagcaa tcccccgctc gtcatcgacg tgcgcaagcg cccggcgttc ctcgccgcgc
     6841 ccgacatgat ccgcggcgcg ctgcgccgcg atcccgagtc gcccggcgca ctgccctgcg
     6901 cggacgacgt cgtcgtgtac tgtgtgcacg gccacgagat gctcgagcac ggcatggtga
     6961 tgtacgacgc gctctacgcc tggtgccgca gcaccaagtc cgaggtgcac acgtggaacc
     7021 ccgccctgat ccggtaagcc tgggcgaggc cacgcgcttc tggctgaagc tcggcttcat
     7081 cagcttcggc gggcccgccg ggcagatcgc gatcatgcac caggagctgg tggagcgccg
     7141 gcgctggatc tcggagaacc gctttctgca cgcgctcaac tactgcatgc tgctccccgg
     7201 gcccgaggcg cagcagctcg cgacctacct cgggtggctg atgcaccgca cctggggcgg
     7261 gatcatcgcg ggcgggctgt tcgtgctgcc ttcagtgttc atcctcatcg cgctgtcgtg
     7321 gatctacctc gtttggggcg aggtgccgct ggtggccgga atcttctacg gcatcaagcc
     7381 ggcggtcgtg gcggtcgtcg ccgcggcagc ctggcgcatc ggcgcgcgca cgctgaagaa
     7441 cgaatggcac tgggggatcg ccgtcgccgc gttcctcgcc ttggccgtcc tcggcgtgcc
     7501 cttccccgcg atcgtcgccg ccgccgccgt catcggctac gccggcgggc gcatcgcgcc
     7561 ggccaagttc tcgggcaacg gcggccacgg cgccggcaag cgcggctatg gcccggcgct
     7621 ggtcgacgac gacacgccga cgccgccaca cgccctattc cgctggccgc gcttcttcgc
     7681 catcctcggc atcgccgccg cgctgtgggc gctcgccctc ggcgcgctgg cgctcgcctt
     7741 cggctggccc ggcacgctga cccagatggg ctggttcttc accaaggcgg cgtttctcac
     7801 cttcggcggc gcctatgcgg tgctgccgta cgtctaccag gccgccgtcg agcaccacca
     7861 gtggctcacg gcgccgcaga tgatcgacgg gctggcgctg ggagagacca cgccggggcc
     7921 gctcatcatg gtggtggcct ttgtcggctt cgtcggcgcc tgggcgcgcg aggcgctcgg
     7981 gcccgaggcg ctggcgctcg ccggcacggc gggcgcgctc gtcgccacct acttcacctt
     8041 cctgccctcg ttcgtgttca tcttcgccgg cgcgccgttc gtcgagacca cgcacggcaa
     8101 cctgcgcttc acggcgccgc tcgccggcat caccgcggcg gtcgtcgggg tggtggtgag
     8161 cctggcggtg ttcttcgcgc ggcacgtctt tttcactgcc gaggcgatcg actggatctc
     8221 gatcgtcctc gccgccgcgg cgctcgctgc gctcctgcgc ttcaaggcgg ggctgatccc
     8281 cgtgatcctc ggctgcggcg ccgctggact gtgctataga gtgctgctca actgaggagg
     8341 agcgccatgg ccggctacgt cgtcgtcgaa gtggatgtca ccgattccgc ggcctacgag
     8401 gagtaccgca agctcgttcc cgccaccatc gccaagtacg gcggcagatt cctcgtgcgg
     8461 ggcggcgcga tcgagagcaa ggaaggcggc tggcagccca agcgcctcgt ggtgctggag
     8521 tttccgtcgc tggaggacgc gcgcaagtgg tatgcgtcgc ccgagtacgc gcccgcgctg
     8581 gcgctgcggc tgaaagccgc gcgctcgaag ctgctgctgg tggaaggcgc gcccccggcc
     8641 taggggcgcg caggccgcgc tacttcttcg ccgcgtcctt ggtggcgccc acgcccatgg
     8701 gcgccgcagg cttcttcgcg tccatgctca tgcctttgtt cttcctgtag ttggcgacag
     8761 cgcgctcctg cgcgatgtcg tc
//
LOCUS       DBNHKE010000086         9753 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_279582, whole genome shotgun sequence.
ACCESSION   DBNHKE010000086 DBNHKE010000000
VERSION     DBNHKE010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_279582"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2129
                     /locus_tag="AC16O9_05465"
     CDS             <1..2129
                     /locus_tag="AC16O9_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516098.1"
                     /translation="APVPLPPAPVARPAPPAVAPVPAAPAPAVPTPAAPPELAQFRFL
                     IRLATFLSREEMQRASPVPGEFSSYEVQISEAQRNGRREYDLVLGYFPSAEAASAARR
                     RLLQRFPRAEVVDLGAPGPTIAAAPKPKPEPALAAAPKPKPTPVPAAPALPRPSVPAA
                     PIASAEIEASAAELLSSARAALEAGNDAPAIDSLNKLLMLPPNSLSRDAQELMGFARE
                     RAGEVAKARAEYELYLKLYPEGAGAARVRERLAALAALPVAQAPVRAARAPERTVTGA
                     LSQYYYGGRTKIETAFDTPTTPDRSTFSGVDQSTLVTNADLSVRNRSAEADSRFVFRD
                     TNSKAFLENQRSYNRLTAAYYDYRSLQNGVSARVGRQSGLSGGLPGRFDGAVAGYGLA
                     QKWRANAAVGRPVEYPQLDAERSFWSANLEYQNLADAWSGNFYYVEQESDGLLDRRAV
                     GTETRYFSARTSLFSVLDYDTSYKQWNVTMVQATYQTEGRTTINLLYDKRRAPTLSTT
                     NAIFGQGTTSLKTLLQTFTEDQLRQQALDITATSTQGVIGFTTPVGKKWQMGADVRLT
                     KVGALPSVEVNGIIVPAQPATGDIYNYSLQAIGSNLYSGRDTSVYNLTYLDGPTQTGY
                     QLSYNNLTALGAWTLEPSLRYYTQKDNQGIELERWTPSLRITYQAGANVALESEYSWE
                     RTVTVGPSSRDETRRGFYYLGYRWNF"
     gene            complement(2137..3291)
                     /locus_tag="AC16O9_05470"
     CDS             complement(2137..3291)
                     /locus_tag="AC16O9_05470"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6516099.1"
                     /translation="MAKKKENRVGRYRILDELGRGAMGVVYRAEDPSLDRIVALKTIA
                     LGDADEDRKAYEKRFMLEAKAAGKLTHPNIVTIFDYGEEEDLAYMAMELLEGQDLRAR
                     IRQGEIPPMEAVEIALQVADGLGFAHEFGVVHRDVKPGNIMLLERGAVKIMDFGIARM
                     RHADTSRTSTGMVLGTPRYMSPEQISGQPVDQRSDIFSLGTVLYEMLTQTTLFAGQDV
                     NQIAHSVTSTEPAPPSHLHPGLPQMLDFVVARALKKDPTVRYQDAYEMAADLRDVLAE
                     MRGRAPARDRGGDDGEKTQTLKMAAGADKLPVAPAANAIARETRLPLSRVFDSEAALR
                     RVEARAKRERFTLPPRRVGLFRRLVQDRLPRRLLAAALVAAGLGALIAAT"
     gene            complement(3294..4079)
                     /locus_tag="AC16O9_05475"
     CDS             complement(3294..4079)
                     /locus_tag="AC16O9_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516100.1"
                     /translation="MAKAKAQAKPPAQPSAPSQAQIQPSVFASVTFLKIHDFGRRPVV
                     EQARLRAQLEAVVAVALQDLREEARLVLDAADGIALVVLDNPRAALAVAERSLAAGAA
                     GLPLAVGINHGPVKQAGKGKNEGLLGDGIAVAAAIAELAGAKKVLLTKEFREALADAH
                     PGAEAALATAGTHTDASLRAHQVFKPDTRGAARRRLRYFAATVVAVVALVGAGAAFRI
                     SVEGLDRYTQRVGAQAKDVVQRLRRDAPQPAAQPAAAARRGTR"
     gene            complement(4158..6149)
                     /locus_tag="AC16O9_05480"
     CDS             complement(4158..6149)
                     /locus_tag="AC16O9_05480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR6516101.1"
                     /translation="MGAAEAFAKLNERQRQAAQAPPTPLLIIAGAGTGKTNTLAHRVA
                     HLVLEGAAPERILLLTFTRRAAVEMTRRASRIVAEAHKGVRLPWSGTFHAVANRLIRK
                     HCRRVGLTENFSVLDRGDAADLMDVVRHELGYSKTEKRFPRKDTCVAIYSHRVNTQGT
                     LEASLKDAFPWCAEWEGELKKLFAAYLERKLANQSLDYDDLLLYWHAMMQDAALAAEI
                     GAAWDHVLVDEYQDTNVLQAEILARLKPDGRGLTVVGDDAQSIYSFRAATVENILSFE
                     ARFGAAVVTLEANYRSTQPVLEAANALMREASRQYQKELRSAKGEGPKPRLVTVADDA
                     AQAGYVVTRVLEAREEGTDLKRQAVLFRSSHHSDVLELELVKRNIPYVKYGGLKFLEA
                     AHVKDALAVLRWADNPRNRVAAWRALQLLPGVGPKGAERAWTAFEAASHAWAALKTAS
                     KAFDALLAGLADPQAPWSGQMQRVREWYAPIMERLYDAPEVRAGDLVQLERIATTYGT
                     REAFLTELALDPPQASSDLAGEPHLDEDYLILSTVHSAKGQEWDAVYLLNVTDGNFPN
                     EFATGKAESIEEERRLLYVAMTRARTRLELVSPLKYYVTQQSRMGDRHVYGAKSRFLT
                     RTVMACLEPVVYGESCETAARAANPSPVDVASRLRDMWA"
     gene            complement(6204..7031)
                     /locus_tag="AC16O9_05485"
     CDS             complement(6204..7031)
                     /locus_tag="AC16O9_05485"
                     /inference="COORDINATES: protein motif:HMM:NF039315.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HIR6516102.1"
                     /translation="MPIAWSMLHNPEPTTLVAARVLAHRGAQWAARAARANLEPVPDD
                     SHSALTWDGAKAALLSQPLKGGTQVGLRIGVLEVIVSTKDKTEAFPLATNTEAEVADW
                     LDGMLAERGLKPAGGLKLPYEMPATNFARAVDEGPRLAALASWFAAGAELFEELRSKY
                     KRYKPGPGPVRCWPHHFDMAFVVELEAQKNGRAIGIGLSPGDDYYAQPYFYLSPYPKP
                     ADTADLPALPPGARWHAREFFGAVVTGVELLALADPQAGLLEALDAAFAESLKRLGL"
     gene            complement(7076..7369)
                     /locus_tag="AC16O9_05490"
     CDS             complement(7076..7369)
                     /locus_tag="AC16O9_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6516103.1"
                     /translation="MNKTFASIKRGLQQAVRHQRGKRMSGFRLHVPPEIDVKAVREKT
                     GLTQEQFAAAFAISLGALRHWERGDRKPRGTALVLLNLIAKEPRAVLRAVAKA"
     gene            complement(7366..7707)
                     /locus_tag="AC16O9_05495"
     CDS             complement(7366..7707)
                     /locus_tag="AC16O9_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR6516104.1"
                     /translation="MLVTVAETPEYVRRAERLLSAAERADLVAYLAANPRAGDLMQGT
                     GGIRKLRWARGGRGKSGGARVIYYFHSEAMPLYLLTVFGKNERANLSKAERNELAALV
                     RVLKAMVEKRP"
     gene            7817..9613
                     /locus_tag="AC16O9_05500"
     CDS             7817..9613
                     /locus_tag="AC16O9_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6516105.1"
                     /translation="MALLTLLDAQLAYGELPLLDRAAFSMEPGERVGLIGRNGTGKSS
                     LLGVIAGAVALDDGELRKSESLRVVRVEQEPTLPHAPTLRASLLLRGHIPSIADERER
                     WRVEARLTEFLHRFALAEDASPQAASGGEAKRAALALALALAPDLLLLDEPTNHLDIA
                     GIEQLEGLLLKQPAAIVVTHDRAFLDRVCTRILELDRGMLRSYPGNYSAYEGVKAGDL
                     AAEAVQRRKFDKFWAEEEAWIRKGIEARRTRNEGRVRRLERLREERAARRERLGTVKL
                     SVSAGPRSGKLVAELTGVAKRYGDRDVVKDLDLLVSRGDRLGLVGPNGAGKTTLLKLI
                     LGTLAPDAGTVRLGTNVAPAYFDQMRAALDPEKTVAETISPGSDWVELAGGKKHVMSY
                     LGDFLFPPRRAASPVRMLSGGERNRLLLARLFARPANVLVLDEPTNDLDIESLELLEA
                     ALQEYEGTLLLVSHDRAFLDNVVASVLAPEGGGAWREYAGGYSDWVRQRPAAAAVEEK
                     AAAKAAARDKSRAKLSYKETRELAALPGEIEALEAEQKALQGKMSAPEYFKHGAEALK
                     EDRRRTEEIEALLLLKLERWEELEAMAKAGVS"
BASE COUNT         1364 a         3468 c         3482 g         1439 t
ORIGIN      
        1 ccgcgcccgt gccgctgccg cccgcgcccg tcgcgcgccc ggcgccgccg gccgtcgcgc
       61 ctgtgcccgc cgcccctgca ccggccgtgc cgacgcctgc cgcgccgccg gaactggcgc
      121 aattccgctt cctgatccgc ctggcgacgt tcctttcgcg cgaggaaatg cagcgcgcga
      181 gcccggtgcc gggggagttc tcgagctacg aagtgcagat ctccgaagcg cagcgcaacg
      241 gccgcaggga gtacgacctg gtgctgggct atttcccgag cgccgaggcc gcgagcgcgg
      301 cgcgccggcg cctgctgcag cgctttccgc gcgccgaggt cgtggacctg ggcgcgcccg
      361 ggccgacgat cgccgcggcg cccaagccca agccggagcc cgcgcttgcc gctgcgccca
      421 agcccaagcc cacgccggtg ccggccgcgc cggcgctgcc ccgcccgagc gtgccggccg
      481 cgccgatcgc ttccgccgag atcgaagcga gcgcggccga actgctctcg agcgcgcgcg
      541 cggcgctcga agcgggcaac gacgccccgg cgatcgacag tctgaacaag ctgctcatgc
      601 tgccgccgaa cagcctgtcg cgcgacgcgc aggagctgat gggcttcgcg cgcgagcgcg
      661 cgggcgaagt ggcgaaagcg cgcgccgaat acgagctgta cctgaagctc taccccgagg
      721 gcgcgggcgc ggcgcgcgtg cgcgagcgcc tcgcggcgct ggccgcgctg ccggtggcgc
      781 aggcgcccgt gcgcgccgcg cgcgcccccg agcgcacggt gaccggcgcg ctctcgcagt
      841 actactacgg cgggcgcacc aagatcgaaa ccgcgttcga tacgcccacc acgccggatc
      901 gctcgacctt ttccggcgtg gaccagtcga cgctggtcac gaacgccgac ctctcggtgc
      961 gcaaccggag cgcggaggcc gactcgcgct tcgtgttccg cgacaccaac agcaaggcct
     1021 ttctcgagaa ccagaggagc tacaaccgcc tcaccgcggc ctattacgac taccgcagcc
     1081 tgcagaacgg ggtctcggcg cgcgtcggcc ggcaatcggg cctctcgggc gggctgcccg
     1141 ggcgcttcga cggcgcggtc gccggctacg gcctcgcgca gaagtggcgc gcgaacgcgg
     1201 cggtgggcag gccggtggag tatccgcagc tcgacgccga gcgcagcttc tggagcgcga
     1261 acctcgagta ccagaacctg gccgacgcct ggagcgggaa cttctactac gtcgagcaag
     1321 agagcgacgg gctgctcgac cggcgtgccg tgggcacgga aacgcgctac ttcagcgcgc
     1381 gcacgtcgct gttttcggtg ctcgactacg acacgagcta caagcagtgg aacgtcacca
     1441 tggtgcaggc gacctaccag accgaggggc gcaccaccat caacctgctc tacgacaagc
     1501 gccgcgcccc cacgctgagc acgacgaacg cgatcttcgg ccagggcacg acctcgctga
     1561 agacgctgct gcagacgttc accgaggacc agctgcgcca gcaggcgctc gatatcacgg
     1621 cgacgtcgac gcagggagtc atcggcttca ccacgcccgt gggcaagaag tggcagatgg
     1681 gcgccgacgt gcgcctgacg aaggtcggcg cgctgccctc ggtggaggtg aacggcatca
     1741 tcgtccccgc gcagcccgcc accggcgaca tctacaacta cagcttgcag gcgatcggct
     1801 ccaacctgta ctcgggccgc gacacgagcg tctacaacct cacctacctc gacggcccga
     1861 cgcagaccgg ctaccagctc tcctacaaca acctcacggc gctcggcgcc tggacgctcg
     1921 agccgtcgct gcgctactac acgcagaagg acaaccaggg aatcgagctc gagcgctgga
     1981 cgccgtcgct gcgcatcacc taccaggccg gcgccaacgt cgccctggaa tccgagtaca
     2041 gctgggagcg caccgtcacc gtcgggccca gctcgcgcga cgagacgcgg cgcggctttt
     2101 actacctcgg ctaccgctgg aacttctgag cgccgctcaa gtcgcggcga tcagcgcccc
     2161 gaggccggcc gccaccagcg ccgcggcgag aagccgccgc ggcagccggt cctgcaccag
     2221 gcgccggaac aaacccacgc gccgcggcgg cagcgtgaag cgctcgcgct tggcgcgcgc
     2281 ctcgacgcgc cgcagcgccg cctcgctgtc gaacacgcgc gacaggggca ggcgcgtctc
     2341 gcgcgcgatg gcgttggccg ccggggcgac gggcagcttg tccgcccccg ccgccatctt
     2401 cagcgtctgc gtcttctcgc cgtcgtcgcc gccgcggtcg cgcgccgggg cgcggccgcg
     2461 catctcggcg aggacgtcgc gcaggtccgc cgccatctcg taggcgtcct ggtagcgcac
     2521 cgtcgggtcc ttcttcagcg cgcgcgcgac gacgaaatcg agcatctgcg gcagtcccgg
     2581 gtgcaggtgc gacggcggcg ccggctcggt gctggtgacg ctgtgcgcga tctggttcac
     2641 gtcctggccg gcgaagagcg tggtctgcgt cagcatctcg tagagcaccg tgccgagcga
     2701 aaagatgtcc gagcgctggt ccaccggctg gccgctgatc tgctcgggcg acatgtagcg
     2761 cggcgtgccg agcaccatgc ccgtgctggt gcgcgaggtg tcggcgtggc gcatgcgcgc
     2821 gatgccgaag tccatgatct tcaccgcgcc gcgctcgagc agcatgatgt tgccgggctt
     2881 cacgtcgcgg tgcacgacgc cgaattcgtg cgcgaagccg aggccgtcgg ccacctgcag
     2941 cgcgatctcc acggcctcca tcggcgggat ctcgccctgg cggatgcgcg cgcgcaggtc
     3001 ctggccctcg agcagctcca tcgccatgta ggcgaggtcc tcctcctcgc cgtagtcgaa
     3061 gatggtgacg atgttggggt gggtgagctt gcccgcggcc ttcgcctcga gcatgaagcg
     3121 cttctcgtag gccttgcggt cctcgtcggc atcgccgagc gcgatggtct tcagcgcgac
     3181 gatgcgatcg agcgacgggt cctcggcgcg gtagaccacg cccatggcgc cgcgccccag
     3241 ctcgtcgagg atgcggtagc ggccgacgcg gttctccttc ttcttcgcca tgtctagcgg
     3301 gtcccgcgcc gcgcggccgc ggcgggctgc gccgccggct gaggcgcatc gcgcctcagc
     3361 ctttgcacca cgtccttcgc ctgcgcgccc acgcgctggg tgtagcggtc caggccctcg
     3421 acggagatgc ggaacgcggc gcccgcgccc accagggcga cgaccgccac gaccgtcgcc
     3481 gcgaagtagc gcaggcgccg gcgcgccgcg ccgcgcgtgt cgggcttgaa gacctggtgc
     3541 gcgcgcaggc tcgcgtcggt gtgcgtgccg gcggtggcga gcgcggcctc cgccccgggg
     3601 tgtgcgtcgg cgagcgcttc gcggaactcc ttggtgagca gaaccttctt cgcgccggcg
     3661 agctcggcga tcgcggcggc cacggcgatg ccgtcgccga gcaggccctc gttcttgccc
     3721 ttgccggcct gcttcacggg accgtggttg atgccgacgg cgagcggcag gccggccgcg
     3781 cccgcggcga gcgagcgctc ggcgaccgcc agcgccgcgc gcgggttgtc gagcaccacc
     3841 agcgcgatgc cgtcggcggc gtcgagcacc aggcgcgcct cttcgcgcag gtcctgcagc
     3901 gccactgcca ccaccgcctc gagctgcgcc ctgaggcgcg cctgctccac caccggccgc
     3961 cggccgaagt cgtggatctt caggaacgtc acgctcgcaa acaccgaggg ctggatctgc
     4021 gcctgcgatg gcgcgctcgg ctgcgccggg ggtttcgcct gtgccttggc cttggccatg
     4081 gccgattcta aatggggaca gacctcattt agccatggcg gcaactgccg ccagcgctaa
     4141 atgaggtctg tccccattta cgcccacata tcccggagtc tcgaagcgac gtccacgggc
     4201 gaggggttcg cagcgcgcgc ggcggtttcg caggattcgc cgtacaccac cggctcgagg
     4261 caggccatga ccgtccgggt gaggaagcgg cttttcgcgc cgtagacgtg gcggtcgccc
     4321 atgcgcgatt gctgcgtcac gtagtacttg agcgggctga cgagctcgag ccgggtcctc
     4381 gcgcgcgtca tggcgacgta gagcaggcgc cgctcctcct cgatgctttc ggccttgccg
     4441 gtggcgaact cgttcgggaa gttgccgtcg gtgacgttga gcaggtacac cgcgtcccac
     4501 tcctggccct tcgccgaatg caccgtggac aggatcaggt agtcctcgtc cagatgcggc
     4561 tcgccggcga ggtccgagct cgcctgcggc ggatcgagcg cgagctcggt gaggaacgct
     4621 tcgcgcgtgc cgtaggtggt ggcgatgcgc tcgagctgca ccaggtcgcc ggcgcgcact
     4681 tcgggcgcgt cgtacaggcg ctccatgatc ggcgcgtacc actcgcgcac gcgctgcatc
     4741 tggcccgacc agggcgcctg cgggtcggcg aggccggcaa ggagcgcatc gaacgccttc
     4801 gaggcggtct tcagcgccgc ccacgcgtgg ctcgcggctt cgaacgcggt ccaggcgcgc
     4861 tcggcgccct tggggccgac gccgggcagc agctgcagcg cgcgccaggc ggcgacgcgg
     4921 ttcctcgggt tgtcggccca gcgcagcacc gcgagcgcgt ccttcacgtg cgcggcctcg
     4981 aggaacttca gcccgccgta cttcacgtag gggatgttgc gcttgacgag ctcgagctcg
     5041 agcacgtccg aatggtgcga gctcctgaag agcaccgcct ggcgcttgag gtcggtgccc
     5101 tcctcgcgcg cctccagcac gcgcgtcacc acgtagcccg cctgcgcggc gtcgtcggcg
     5161 acggtcacga gccgcggctt ggggccctcg cccttggccg agcgcagttc cttctggtac
     5221 tggcggctcg cctcgcgcat cagcgcgttg gccgcctcga gcaccggctg cgtcgagcgg
     5281 tagttcgctt ccagcgtcac caccgccgcg ccgaagcgcg cctcgaagga caggatgttc
     5341 tccaccgtcg cggcgcgaaa cgagtagatc gactgcgcgt cgtcgcccac cacggtgagg
     5401 ccccgcccgt cgggcttgag gcgcgcgagg atctcggcct gcaggacgtt ggtgtcctgg
     5461 tattcgtcca ccagcacgtg gtcccaggcg gcgccgatct ccgcggcgag cgccgcgtcc
     5521 tgcatcatcg cgtgccagta aagcagcagg tcgtcgtagt cgagcgattg attggcgagc
     5581 ttgcgctcga ggtacgcggc gaagagcttc ttcagctcgc cctcccactc cgcgcaccag
     5641 gggaacgcgt ccttcagcga agcttcgagc gtgccctgcg tgttcacgcg gtgcgagtag
     5701 atcgcgacgc aggtgtcctt cctgggaaag cgcttctcgg tcttcgagta gccgagctcg
     5761 tggcgcacca cgtccatcag gtcggccgcg tcgccgcgat cgagcacgct gaagttctcg
     5821 gtgagcccca cccggcggca gtgcttgcgg atgaggcggt tggcgaccgc gtggaaggtg
     5881 cccgaccacg gcaggcgcac gcccttgtgc gcctcggcca cgatgcggct cgcgcggcgc
     5941 gtcatctcga cggccgcgcg gcgcgtgaag gtgagaagga ggatgcgctc cggcgccgcg
     6001 ccctcgagca cgaggtgcgc gacgcggtgc gcgagcgtgt tggtcttgcc ggtgccggcg
     6061 ccggcgatga tgagcagcgg ggtcggggga gcctgcgcgg cctggcgctg ccgctcgttc
     6121 agcttcgcga atgcctccgc cgctcccatg gggggcgact ataggccaac actgtatgtt
     6181 tatccagtcg cgcggggcgc ggcctagagg ccgaggcgct tcaggctttc cgcgaacgcg
     6241 gcgtcgagcg cttcgagcag gcccgcttgc gggtcggcga gcgcgagcag ctccaccccg
     6301 gtcaccaccg cgccgaagaa ttcgcgcgcg tgccagcgcg cgcccggcgg cagcgcgggg
     6361 aggtccgccg tgtccgcggg cttcggatag gggctgaggt agaagtaggg ctgcgcgtag
     6421 tagtcgtcgc cgggcgagag gccgatgccg atcgcgcgcc cgttcttctg cgcctccagc
     6481 tcgacgacga aggccatgtc gaagtggtgc ggccagcagc gcaccggccc cgggccgggc
     6541 ttgtagcgct tgtacttgct tcgcagctcc tcgaacagct cggcgccggc ggcgaaccag
     6601 ctcgcgagcg cggcaaggcg cgggccctcg tccaccgcgc gggcgaagtt ggtcgcgggc
     6661 atctcgtagg gcagcttgag gccgccggcg ggcttcaggc cccgctcggc gagcatgccg
     6721 tcgagccagt cggcgacctc ggcctcggtg ttggtcgcga gcgggaatgc ttccgtcttg
     6781 tccttcgtgg agacgatcac ttccagcacc ccgatcctga ggcccacctg cgtgccgccc
     6841 ttcagcggct ggctgagcag cgccgccttg gcgccgtccc aggtgagcgc cgagtggctg
     6901 tcgtcgggca ccggctcgag gttggcgcgc gcggcgcgcg ccgcccactg cgcgccgcgg
     6961 tgcgcgagca cgcgcgccgc cacgagcgtc gtcggctcgg ggttgtgcag catggaccag
     7021 gcgatcggca tctcttgagg gtattcgctt cgcgggtggc cggcttacag ggccctcagg
     7081 ccttcgccac cgcccgcagc accgcccgcg gctccttcgc gatcaagttg agcaggacga
     7141 gggcggtgcc gcgcggcttg cggtccccgc gctcccagtg ccgcagcgcg ccgaggctga
     7201 tggcaaaagc cgccgcgaac tgttcctgcg tcagcccggt cttctcgcgc accgccttca
     7261 cgtcgatctc gggcggcacg tgcagcctga atccggacat ccgcttgccc cgctggtggc
     7321 ggaccgcctg ctgcagtccg cgcttgatgc ttgcgaaagt cttgttcatg gtcgcttctc
     7381 caccattgcc ttgagcacgc ggaccagcgc cgccagctcg ttgcgctctg ccttgctcag
     7441 gttcgcccgc tcgttcttgc cgaataccgt cagcaggtag agcggcatgg cctcgctgtg
     7501 aaagtagtag atcacccgcg cgccgccgct cttgccgcgc ccgccgcgcg cccagcgcag
     7561 cttgcggatg ccgccggtgc cctgcatcag gtcgcccgcc ctcgggttcg cggcgaggta
     7621 tgccacgagg tcggcgcgct ccgctgccga cagcaggcgc tccgcacggc gcacgtactc
     7681 cggcgtctcc gcgacggtca ccagcatact aatccaatgg attagtttcg tccagcggca
     7741 tgaatccccc tgctccctac caacgcgcgg cggcgccccg ggttgacgcc cccggggccg
     7801 ttacactggc gcccccatgg ctttactgac cctgctcgat gcgcagctcg cctacgggga
     7861 gctgccgctc ctcgaccgcg cggcgttctc gatggagccg ggcgaacggg tcgggctgat
     7921 cggccgcaac ggcacgggaa agtcgtcgct cctcggcgtg atcgccggcg ccgtggcgct
     7981 cgacgacggc gagctgcgaa agagcgagtc gctgcgcgtg gtgcgcgtgg agcaggagcc
     8041 gacgctcccg cacgcgccga cgctgcgcgc gagcctgctg ctgcgcggac acattccctc
     8101 gatcgcggat gagcgcgagc gctggcgggt ggaggcgcgg ctcacggagt tcctgcaccg
     8161 cttcgccctg gcggaggacg cctcgccgca ggcggcgtcc gggggcgaag caaagcgcgc
     8221 ggcgctggcc ctcgcgctgg cgctcgcgcc ggacctgctg ctcctcgatg agcccaccaa
     8281 ccacctcgac atcgccggaa tcgagcagtt ggaggggctg ctcctgaagc agccggcggc
     8341 gatcgtggtg acgcacgacc gcgcgttcct cgatcgcgtg tgcacgcgga tcctggagct
     8401 cgaccgcggg atgctgcgct cgtacccggg gaattattcg gcgtacgagg gcgtgaaggc
     8461 cggggacctc gctgcagagg ccgtgcagcg caggaagttc gacaagttct gggccgagga
     8521 agaggcctgg atccgcaaag gcatcgaggc gcgccgcacg cgcaacgaag gccgcgtgcg
     8581 gcgccttgag cgcctgcgcg aagagcgcgc cgcgcggcgc gagcggctgg ggacggtgaa
     8641 gctatcggtc tccgcgggcc cgcgctcggg aaagctggtg gcggagctga ccggcgttgc
     8701 caagcgctac ggcgatcgcg acgtggtgaa ggacctggac ctgctcgtct cgcgcggcga
     8761 ccggctcggg ctggtggggc cgaacggcgc ggggaagacc accctcctca agctcatcct
     8821 cggcacgctc gcgcccgatg cggggacagt gcgcctgggc accaacgtcg cgccggcgta
     8881 cttcgaccag atgcgcgcgg cgctcgatcc ggagaagacc gtggccgaga ccatctcgcc
     8941 ggggtcggac tgggtggagc tcgccggcgg gaagaagcac gtcatgagct acctcggcga
     9001 cttcctgttc cctccccggc gcgccgcctc gcccgtgcgc atgctctcgg gcggcgagcg
     9061 caaccggctg ctcctcgcgc ggctcttcgc gcggcccgcg aacgtgctgg tgctcgacga
     9121 gccgacgaac gacctggaca tcgagtcgct cgagctgctg gaagcggcgc tgcaggagta
     9181 cgaaggcacg ctcctcctcg tgagccacga ccgcgcgttc ctcgacaacg tggtcgcctc
     9241 ggtgctcgcg cccgagggcg gcggcgcctg gcgcgagtac gcgggcgggt attcggactg
     9301 ggtgcgccag cgccccgcgg cggcggcggt ggaggaaaag gcggcggcga aggcggccgc
     9361 gcgcgacaag agccgcgcca agctttccta caaggagacg cgcgagctgg cggcgctgcc
     9421 cggggagatc gaggcgctgg aggccgaaca gaaggcactg cagggcaaga tgagcgcgcc
     9481 ggagtatttc aagcacgggg cggaggcgct gaaggaggac agaaggcgca ccgaggagat
     9541 cgaggcgctg ctgctgctga agctggagag gtgggaggag ctggaggcga tggcgaaggc
     9601 gggggtgtcc tagccggcgc ggcgggatcg gcggcgtgcg ccttgagctg gccggtgcgc
     9661 agcaggtttt ccaaggtcat cctcgttccc cacgatgaag atcttgcgcc cggccaggac
     9721 ccgcttgagg aacgggtccc cggcggcgag ctt
//
LOCUS       DBNHKE010000087         3204 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_332555, whole genome shotgun sequence.
ACCESSION   DBNHKE010000087 DBNHKE010000000
VERSION     DBNHKE010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_332555"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC16O9_05505"
     CDS             <1..314
                     /locus_tag="AC16O9_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6516106.1"
                     /translation="ADMHDIGDALVAAGFAAPVMDMEMLTLQYPDPAGLAADLRASGQ
                     TLALRARARGLAGRGLRARLAAAGASASIEVIYGHAWKGVPRKTADGRGIVHFERRPL
                     P"
     gene            373..858
                     /locus_tag="AC16O9_05510"
     CDS             373..858
                     /locus_tag="AC16O9_05510"
                     /inference="COORDINATES: protein motif:HMM:NF021517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HIR6516107.1"
                     /translation="MSTLELEFPRRETQFAATRAGFRVVLKRNCSISPRGLLLAFALL
                     AALALGIAAAFAALGAWLILPFAGLELLLLGAAFWVTARHAADYERIERARDRLTVDV
                     SEGERLRRYEFDAGRLRVRLGERGRVLLDAPQARLEIGRHLDAASRAGFAAELGKRLQ
                     S"
     gene            876..2003
                     /gene="coxB"
                     /locus_tag="AC16O9_05515"
     CDS             876..2003
                     /gene="coxB"
                     /locus_tag="AC16O9_05515"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6516108.1"
                     /translation="MLKKLFVGALGLALCGVALAAEWNLQPAASKIAEDIHWLHEAVM
                     VLVVVLFVGVFGFMFWSCYAHRKSVGHKADQFHENTTVEVIWTVVPALILVLIAWPVT
                     KVVIAQKDTANPDLTIKVTGIQWKWAYDYLKGEGEGISFVSTLATPRAQIENREAKGE
                     NYLLEVDNELVVPVGKKVRMLTTAADVIHSWWVPAFGAKQDAIPGFIRDIWFTPTKTG
                     TYRSQCVELCGKDHGFMPIVVRVVSQEDYTKWVGEQKQLLAKAAEDETKTYTLAELKS
                     RGEKVYAANCVACHQANGKGVPPAFPALDGSKLVLGPKDAQIDVILNGVTKNGTPTAM
                     AAFGKQLSNAELAAVITYTRNNWGNSTGEAVQPADIKARRK"
     gene            2006..>3204
                     /locus_tag="AC16O9_05520"
     CDS             2006..>3204
                     /locus_tag="AC16O9_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR6516109.1"
                     /translation="MSAVVDTHGHEHGHGDHHGGYGGIMRWVTTTNHKDIGTMYLWFS
                     FVMFMVGGIMALVIRAELFSPGLQIVDPDFFNQMTTMHGLIMVFGAIMPAFVGFANWL
                     IPMQIGAPDMAFARMNNWSFWILPFAGALLIISFFTPGGAPGVGWTLYAPLTVQMGIG
                     MDLTIFAVHLLGMSSIMGSINIVTTILNMRAPGMTLMRMPLFCWTWLITAYLLIAVMP
                     VLAGAVTMTLTDRHFGTSFFNAAGGGDPVLYQHIFWFFGHPEVYIIALPAFGVVSQIL
                     PAFSRKPLFGYASMVYATSAIAILSFIVWAHHMYTVGMPVTGQLFFMYATTLIAVPTG
                     VKVFNWVATMWKGSLTFETPMLFACGFLFLFTLGGFTGLVLSLVPIDIQLHDTYYVVA
                     HFHYVMVA"
BASE COUNT          504 a         1033 c         1102 g          565 t
ORIGIN      
        1 tcgccgacat gcacgacatc ggcgacgcgc tggtggcggc gggcttcgcg gcgccggtga
       61 tggacatgga aatgctcacg ctgcagtacc cggatccggc gggcctcgct gccgacctgc
      121 gcgcgagcgg ccagacgctc gcgctgcgcg cgcgcgcgcg cggcctcgcc ggacgtggcc
      181 tgcgcgcgcg gctcgccgcc gcgggcgctt ctgcctccat cgaggtgatc tatgggcacg
      241 cgtggaaagg cgtgccgcgc aagacggcgg acggacgcgg catcgtgcat ttcgagcgcc
      301 ggcctctgcc ttgagcggcc aaactatgct aatgtttcgc cgcgtttttt ttgcggcacg
      361 acgaaatcat cggtgagcac cctggagctg gaattcccgc ggcgcgagac gcaattcgcg
      421 gccacgcgcg cgggcttccg ggtggtgctg aagcgcaact gctcgatttc gccgcgtggg
      481 ctgctcctcg ctttcgcctt gcttgcggcg ctggcgctgg gcatcgccgc ggcgttcgcg
      541 gcgctggggg cgtggctgat cctgccgttc gcgggtctcg agctgctgct gcttggcgcc
      601 gcgttctggg tcacggcgcg ccacgccgcg gactatgagc gcatcgagcg cgcgcgcgac
      661 cggctcacgg tggacgtgag cgagggcgag cgactgcgcc gctacgagtt cgacgcgggg
      721 cgcttgcgcg tgcggctggg cgagcgcggg cgggtgctgc tcgatgcgcc gcaggcgcgg
      781 ctggagatcg gccggcatct ggacgcggca tcgcgcgccg gatttgcggc cgaactgggc
      841 aaacgattgc agagctgatc ggggaagggt cgaagatgct gaaaaagctg ttcgtgggcg
      901 cgctggggct ggcgctatgc ggcgtggcgc tggccgcgga gtggaacctg cagccggcgg
      961 cgtcgaagat cgccgaggac atccactggc tgcacgaggc cgtcatggtg ctggtggtgg
     1021 tgctgttcgt gggtgtgttc ggcttcatgt tctggtcgtg ctacgcgcat cgcaagtcgg
     1081 tcgggcacaa ggccgatcag ttccacgaga acaccactgt cgaggtgatc tggacggtgg
     1141 tcccggcgct gatcctggtg ctgatcgcct ggccggtcac caaggtggtg atcgcgcaaa
     1201 aggacaccgc gaatcccgac ctcaccatca aggtcaccgg catccagtgg aagtgggcct
     1261 acgactacct gaagggcgag ggcgagggca tcagcttcgt ctccacgctc gccacgccgc
     1321 gcgcgcagat cgagaaccgc gaggcgaagg gcgagaatta cctgctcgag gtcgacaacg
     1381 agctcgtggt cccggtgggc aaaaaggtgc gcatgctgac caccgccgcc gacgtgatcc
     1441 actcctggtg ggtgccggcg ttcggcgcca agcaggacgc gatccccggc ttcatccgcg
     1501 acatctggtt cacgcccacc aagacgggca cctaccgcag ccagtgcgtc gagctctgcg
     1561 gcaaggacca cggcttcatg ccgatcgtgg tgcgcgtggt gtcgcaggag gactacacga
     1621 agtgggtcgg ggagcaaaag cagcttctcg ccaaggcggc cgaggatgag accaagacct
     1681 atacgctggc cgagctcaag tcgcgcggcg agaaggtgta cgccgccaac tgcgtcgcct
     1741 gccaccaggc gaacggcaag ggcgtgccgc cggcgttccc ggcgctcgac ggctccaagc
     1801 tggtgctcgg gccgaaggac gcgcagatcg acgtcatcct gaacggcgtg acgaagaacg
     1861 gcacgccgac ggcgatggcc gcgttcggca agcagctctc gaacgccgag ctcgccgcgg
     1921 tcatcaccta tacgcgcaac aattggggca acagcaccgg cgaggccgtg cagcccgccg
     1981 acatcaaggc ccggaggaaa tagcgatgag cgcagtcgta gacacccacg gacacgagca
     2041 cggccatggc gaccaccatg gcggctacgg cggcatcatg cgctgggtca ccaccaccaa
     2101 ccacaaggac atcggcacga tgtacctgtg gttcagcttc gtcatgttca tggtgggcgg
     2161 aatcatggcg ctggtgatcc gcgccgagct gttctcgccg ggactgcaga tcgtcgaccc
     2221 ggatttcttc aaccagatga ccaccatgca cggcctgatc atggtgttcg gggcgatcat
     2281 gccggccttc gtcggcttcg ccaactggct gatcccgatg cagatcggcg cgcccgacat
     2341 ggccttcgcg cgcatgaaca actggtcgtt ctggatcctg ccgttcgccg gcgcgctgct
     2401 catcatctcg ttcttcacgc ccggcggtgc gcccggcgtg ggctggacgc tgtatgcgcc
     2461 gctcaccgtg cagatgggca tcggcatgga cctgacgatc ttcgcggtgc acctgctggg
     2521 gatgagctcg atcatgggct cgatcaacat cgtcaccacc atcctcaaca tgcgcgcgcc
     2581 ggggatgacg ctgatgcgga tgccgctgtt ctgctggacc tggctgatca ccgcctacct
     2641 gctgatcgcg gtgatgccgg tgctcgccgg cgcggtcacc atgacgctca ccgaccggca
     2701 cttcggcacc agcttcttca acgccgccgg cggcggcgac ccggtgctgt accagcacat
     2761 cttctggttc ttcggccatc ccgaggtcta catcatcgcg ctgcccgcgt tcggcgtggt
     2821 gtcgcagatc ctgccggcgt tctcgagaaa gccgctgttc ggctacgcct cgatggtgta
     2881 tgcgacctcg gcgatcgcga tcctgtcgtt catcgtctgg gcgcaccaca tgtacacggt
     2941 gggcatgccg gtgaccgggc agctgttctt catgtacgcg accacgctga tcgcggtgcc
     3001 caccggcgtg aaggtgttca actgggtggc gacgatgtgg aagggctcgc tcaccttcga
     3061 gacgccgatg ctgttcgcct gcggcttcct gttcctgttc acgctcggcg ggttcacggg
     3121 cctggtgctg tcgctggtgc cgatcgacat ccagctgcac gacacctact acgtggtggc
     3181 gcacttccac tacgtgatgg tggc
//
LOCUS       DBNHKE010000088         7117 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_332565, whole genome shotgun sequence.
ACCESSION   DBNHKE010000088 DBNHKE010000000
VERSION     DBNHKE010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_332565"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC16O9_05525"
     CDS             complement(<1..201)
                     /locus_tag="AC16O9_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6516110.1"
                     /translation="MLKLYGQYRSRAFRVAWLCKESSIPYEHHNVTINVDGATCKEPW
                     YAALNPNKRVPTIDDDGLVVWES"
     gene            284..679
                     /locus_tag="AC16O9_05530"
     CDS             284..679
                     /locus_tag="AC16O9_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6516111.1"
                     /translation="MSKPKPHLEFVGVDMKAGWESPGANYPPGFTQKVLASDIDEKAK
                     RGSRTRLLKLEPGAYSTEPFVHDHWEEVFLIQGDLIVGNDAKGQGGQSSQAFTYACRP
                     PGVHHGPFASKGGCLLYEIHYYETSGKQK"
     gene            688..2145
                     /locus_tag="AC16O9_05535"
     CDS             688..2145
                     /locus_tag="AC16O9_05535"
                     /inference="COORDINATES: protein motif:HMM:NF023222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HIR6516112.1"
                     /translation="MLKTGAEHLKSLRDGRVVYIGGEKVDDVTTHPAFRNAARSMAAI
                     YDLKAANPEFSITEGKEKFSAYFLRAKTKADLEKRTQLHRAIADMSHGLLGRSPDHVS
                     SFVTGMAMNPAVFGKFADNLTRYYEHMRREDVYGVYAVIPPQAARNPEFYVKQNLPIP
                     TLRVVREDDDGVVISGMKMLATGAVFANEIWIGNLIPLAPNQLAESITCAIPVNAPGV
                     TLWSRKPFELHAPNEFDNPLAWRFDETDSMVMCDEVKVPWERVFVHNDAVLARDIYIK
                     TPGHCYGNHQSNVRFHSKMQLIVGLASKIAQASGANEVPAVREVLGRFSALEAALGGM
                     IAGQIQDAEQWPKGFMTFNRRYMYAALNWCTESHSQIIDALRELCGGGIFQMPADITV
                     MHDPKLKAQFETYWQTPQLPALERMKLYKLAWDLIGSEFAGRHLQYEKFYAGASFIVR
                     NHNFREAPWDHYHKVVEDLMASYDVPAAKGTRKVA"
     gene            complement(2103..3530)
                     /locus_tag="AC16O9_05540"
     CDS             complement(2103..3530)
                     /locus_tag="AC16O9_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6516113.1"
                     /translation="MRLVPRSLFGRLVLILLGGLAVALLAAAYLNLTERDQLLYRASG
                     MQTAQRIADVSRLLDSVTPEERRRIAALFDAPPLVISLERPALPPAAPPAEADLRLSM
                     FTSVLGYALGGDLRVHAVRPARAPAGYRAPPRMPMMQHPMAGPGMPMMHADGAFFLVQ
                     VALRDGTLVTFDSVVSPEAAQVPLRFALALLVLFGTVVALSLVAVRWVTGPLSALAEA
                     AERLGEDIHRPPLAETGPLEARRAARAFNTMQQRLQRFINDRTRILTAMSHDLKTPIT
                     RLRLRTELLDDEALRAKFAKDLQDMESMVTQTLDFMRDASAAEPVQQVDLMALLESLQ
                     RDFQDTGNAVEIEGRIAHPFSGRPLALRRCLTNLVDNALRYGKRATLQAEEAPGRITI
                     RVLDEGPGMSAAEFERAFEPFFRGEASRSRETGGSGLGLGIARNIARAHGGELRLRNR
                     AQGGLEAILELPQATLRVPLAAGTS"
     gene            complement(3530..4252)
                     /locus_tag="AC16O9_05545"
     CDS             complement(3530..4252)
                     /locus_tag="AC16O9_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516114.1"
                     /translation="MESPDHILVVDDDAEIRNLLGEYLEKNGCKATVVADGKGMRAAL
                     ARAKVDLIVLDLMLPGEDGLELCRKLRADADTPVIMLTARGEETDRIVGLEIGADDYL
                     AKPFSPRELLARIKSVLRRYRSLPRNLRAEDARAFAFGAWRLDTVARHLVSGQGVVTP
                     LSGAEFRLLRIFLEHPNHVLSRDQLMMLTHGREAEPFDRSIDNQVSRLRHRLGDDPGE
                     PRIIKTVRGEGYVLAVPVEMER"
     gene            4428..5036
                     /locus_tag="AC16O9_05550"
     CDS             4428..5036
                     /locus_tag="AC16O9_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR6516115.1"
                     /translation="MRKTTKIAMAVGAALTLGLATAELTAHPSDEGWGGYGMHGYGMH
                     GDGMRGYGMHGDGMHGYGMGPGGGMGYGMPGYGRGYGAYPGAAGERLAGLKAELGITA
                     EQEAAWQAFVNSAKQREEAREAFFAKRREAADAGTLPERLAQRDEFFKQHQAERQAQT
                     AALKELYAALSPEQKAIADERFGGFGPRYGAGYGRGFGGRSR"
     gene            complement(5102..5959)
                     /locus_tag="AC16O9_05555"
     CDS             complement(5102..5959)
                     /locus_tag="AC16O9_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6516116.1"
                     /translation="MLHKLLLALLLVPGLACAAAGAPRLESLQVEIWPEYDRPAALVI
                     LKGELASDVGLPVTLTLRIPASSGGPSAVAFAPQKTGPLLNLAHDRSAAADAIALRFT
                     TPERFFHVEFYDPLVTGTPARSYKYVWPGDLAVGALQVVVQEPAAATDIAVQPALADL
                     STGIDGLRYRSALLGPVPQGQARSIEIRYAKSDPRTSAEILKAGTPASSPGAGTSSEE
                     RDAFRRFLVSGAIALLLVGSGLVYLFWWRRRERPGPGGNAGARFCRKCGGPAAAGDRF
                     CSKCGAALA"
     gene            6013..6540
                     /gene="hemG"
                     /locus_tag="AC16O9_05560"
     CDS             6013..6540
                     /gene="hemG"
                     /locus_tag="AC16O9_05560"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HIR6516117.1"
                     /translation="MSRILFLYSTVDGHTLKICQRLQQIVEGAGHRAALEVLEAHADM
                     DLEPFDRIVVGASIRYGKHRPEVAEFMRRRRRILEAKPSAFFSVNIVARKAGKDTPQT
                     NPYVVKFLRQIGWRPALVAVFAGKLDYPRYGFWDRQIIRFIMLLTRGPTDPRAVVEFT
                     DWAEVDAFGRRLAAI"
     gene            complement(6537..>7117)
                     /locus_tag="AC16O9_05565"
     CDS             complement(6537..>7117)
                     /locus_tag="AC16O9_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6516118.1"
                     /translation="DFRGVSAHGSMPELGVNAIYKAARVVSGLAAFDFRQPPHAVMGA
                     ATLSVGTFEGGSGVNLVPDAARIGVDVRTLPGMDHAALLARLRAAMGEDVAIDTFSDL
                     PAVWTAPEAEWMQRVFEICTPFLGKPPEVRTATYMTDAANLRKVYGDVPTVVLGPGEP
                     QMAHQTDEYCSMERIRQSVEIYEKIIADWCGA"
BASE COUNT         1163 a         2425 c         2500 g         1029 t
ORIGIN      
        1 cgactcccac acgacgaggc cgtcgtcgtc gatggtcggc acgcgcttgt tcgggttgag
       61 cgccgcgtac cagggctcct tgcaggtcgc cccgtcgacg ttgatggtga cgttgtgatg
      121 ctcgtagggg atgctcgatt ccttgcacag ccaggccacg cggaaggcgc gcgagcggta
      181 ctggccgtag agcttcagca tgttcttcct ctcgggggat tgattgacaa agacattgtg
      241 cgaagggaga ctcccccgag tcaacgccag ttcgaggaag cgcatgtcca agcccaagcc
      301 gcacctggag ttcgtcggag tcgacatgaa agcgggctgg gagtcgccgg gcgcgaacta
      361 cccgcccggc ttcacgcaga aggtgctcgc ttcggacatt gacgagaagg ccaagcgggg
      421 cagccgcacg cgcctgctca agctcgagcc cggcgcctac tcgaccgagc ccttcgtgca
      481 cgatcactgg gaggaggtgt ttttgatcca gggagacctg atcgtgggca acgacgcgaa
      541 agggcagggc gggcagtctt cgcaggcctt cacttacgcc tgccggcccc cgggcgtgca
      601 ccacggcccg ttcgcgagca agggcggctg cctgctctac gagatccact attacgaaac
      661 cagcggcaag cagaagtagg gggcgtcatg ctgaagaccg gagccgagca cctgaagtcc
      721 ctgcgcgacg ggcgcgtggt ctacatcggc ggggagaagg tggacgacgt caccacgcac
      781 cctgcctttc gcaatgccgc gcgctcgatg gctgcgatct atgacttgaa ggcggccaat
      841 cccgagttca gcatcacgga gggcaaggag aagttcagcg cctacttcct gcgcgccaag
      901 accaaggcgg acctcgagaa gcgcacgcag ctgcaccgcg ccatcgccga catgagccac
      961 ggcctgctcg gccgctcgcc cgaccatgtc tccagcttcg tcaccggcat ggcgatgaat
     1021 ccggcggtat tcggcaagtt tgccgacaac ctcacgcgct actacgagca catgaggcgc
     1081 gaggacgtct acggcgtgta cgcggtgatc ccgccgcagg cggcgcgcaa tcccgagttc
     1141 tacgtcaagc agaacctgcc catcccgacc ctgcgcgtgg tgcgcgagga cgacgacggg
     1201 gtggtgatct cgggcatgaa gatgctcgcc acgggcgccg tcttcgccaa cgagatctgg
     1261 atcggcaacc tgatcccgct cgcgcccaac cagctcgccg agtcgatcac ctgcgcgatt
     1321 cccgtgaacg cgccgggcgt gacgctgtgg tcgagaaagc ccttcgagtt gcacgcgccg
     1381 aacgagttcg acaacccgct cgcctggcgc ttcgacgaga ccgactcgat ggtgatgtgc
     1441 gacgaggtga aggtgccctg ggagcgcgtg ttcgtgcaca acgacgcggt gctcgcgcgc
     1501 gacatctaca tcaagacccc gggccactgc tacggcaacc accagtcgaa cgtgcgcttc
     1561 cattccaaga tgcagctgat cgtggggctg gcgagcaaga tcgcgcaggc ctcgggcgcg
     1621 aacgaggtgc cggcggtgcg cgaggtgctg ggacgcttct cggcgctcga agccgcgctc
     1681 ggcggcatga tcgcgggcca gatccaggac gccgagcaat ggccgaaggg cttcatgacc
     1741 ttcaaccggc gctacatgta cgcggcgctc aactggtgca ccgagagcca ctcgcagatc
     1801 atcgacgcgc tgcgcgagct ctgcggcggc ggcatcttcc agatgcccgc cgacatcacc
     1861 gtgatgcacg acccgaagct caaggcgcag ttcgaaacct actggcagac cccgcagctg
     1921 cccgcgctcg agcgcatgaa gctctacaag ctcgcctggg acctgatcgg ctcggagttc
     1981 gccggccggc acctgcagta cgagaagttc tacgccgggg cctcgttcat cgtgcgcaac
     2041 cacaacttcc gcgaggcgcc ctgggaccac taccacaagg tggtcgagga cctgatggcg
     2101 agctacgacg tgccggcggc caaggggacg cgcaaggtcg cctgaggcag ctcgagaatc
     2161 gcctccaggc cgccctgcgc ccggttgcgc agcctgagct cgccgccgtg cgcgcgcgcg
     2221 atgttgcgcg cgatgcccag gccgaggccg ctgccgccgg tctcgcggct gcgcgaggcc
     2281 tcgccgcgga agaagggctc gaacgcgcgc tcgaattccg ccgcggacat gccgggcccc
     2341 tcgtccagca cgcggatggt gatgcgcccg ggcgcctcct cggcctgcag ggtcgcgcgc
     2401 ttgccgtagc gcaaagcgtt gtcgaccagg ttggtgaggc agcggcgcag cgcgagcggc
     2461 cggccgctga acgggtgcgc gatgcggccc tcgatctcga ccgcgttccc cgtgtcctgg
     2521 aaatcgcgct gcaggctttc cagcagcgcc atgagatcga cctgctgcac cggctccgcc
     2581 gcgctcgcgt cgcgcatgaa gtccagcgtc tgcgtcacca tcgactccat gtcctggaga
     2641 tccttggcga acttggcgcg cagcgcctcg tcgtcgagca gctcggtgcg cagccgcagc
     2701 cgcgtgatcg gcgtcttcag gtcgtgcgac atggccgtga gaatgcgcgt gcggtcgttg
     2761 atgaagcgct gcaggcgctg ctgcatcgta ttgaacgcgc gcgccgcgcg ccgcgcctcg
     2821 agcggtccgg tctcggcgag cggcgggcgg tggatgtcct cgcccagccg ttccgcggct
     2881 tcggcgagcg ccgagagcgg gccggtcacc cagcgcaccg cgaccagaga cagcgcgaca
     2941 acggtgccga acagcacgag cagcgccagc gcgaagcgca gcggcacctg cgcggcctcg
     3001 ggggacacga ccgagtcgaa agtcaccagg gtgccgtcac gcagcgcgac ctgcaccagg
     3061 aagaacgcgc cgtccgcgtg catcatcggc atgccgggcc cggccatcgg atgctgcatc
     3121 atcggcatgc gcggcggcgc gcggtagccc gccggtgcgc gcgcggggcg caccgcgtgc
     3181 acgcgcaggt ccccgccgag cgcgtagccc aggacgctcg tgaacatgga cagccgcaga
     3241 tcggcctcgg ccggcggcgc cgcgggcggc agcgcgggcc gctcgagcga gatgacgagc
     3301 ggcggcgcgt cgaacaacgc ggcgatgcgg cgccgctcct cgggagtgac cgagtcgagc
     3361 agccggctga cgtcggcgat gcgctgcgcc gtctgcatgc cgcttgcgcg gtagagcagc
     3421 tggtcgcgct cggtcaggtt gaggtaagcg gccgccagca gcgcgaccgc gaggccgccg
     3481 agcaagatga ggaccagccg cccgaacagg ctgcgcggca caaggcgcat cagcgctcca
     3541 tctcgaccgg cacggcgagc acgtagccct cgccgcgcac ggtcttgatg atgcgcggct
     3601 cgcccgggtc gtcgccgagg cggtggcgca gccggctcac ctggttgtcg atcgagcggt
     3661 cgaacggctc ggcttcgcgg ccgtgcgtga gcatcatcag ctggtcgcgg ctcagcacgt
     3721 gattggggtg ctcgaggaag atgcgcagca ggcggaattc cgcaccgctc agcggggtca
     3781 ccacgccctg gcccgacacc aggtgccgcg cgaccgtgtc caggcgccag gcgccgaagg
     3841 cgaaggcgcg cgcgtcctcg gcgcgcaggt tgcgcggcag gctgcgatag cggcgcagca
     3901 cgctcttgat gcgcgcgagc agctcgcgcg ggctgaaggg cttggcgagg tagtcgtcgg
     3961 cgccgatctc caggccgacg atgcggtcgg tctcctcgcc ccgcgccgta agcatgatca
     4021 ccggcgtatc cgcgtcggcg cgcagcttgc ggcacagctc gagcccgtcc tcgccgggca
     4081 gcatcaggtc gagcacgatg aggtcgacct tggcgcgcgc cagcgcagcc cgcatgccct
     4141 tgccgtcggc gacgacggtc gccttgcagc cgttcttctc caggtattcg ccgagcaggt
     4201 tgcggatctc cgcgtcgtcg tcgacgacca ggatgtggtc ggggctttcc atgggcattc
     4261 ttatagacgc cggcgcggcc gttgtggggc ggatttgtag cgtactgtaa ccggcgccgc
     4321 tgccgataca cgggcgtaca aaatcacgac gcggcgctta caccggcgtg gcgtaatggg
     4381 aaccgcagca agacaggttt ccacacttca cgaaaaggag ttccatcatg agaaagacca
     4441 ccaagatcgc catggccgtc ggcgccgcgc tgacgctcgg actggccact gcggaattga
     4501 ccgcgcaccc ctccgacgag ggctggggcg gctacggaat gcacggctac gggatgcacg
     4561 gcgacggaat gcgcggctac gggatgcacg gcgacgggat gcacggctac ggcatgggac
     4621 ccggcggcgg gatgggttac ggaatgccgg gttacggcag gggctacggc gcctatcccg
     4681 gcgcggccgg ggagcgcctc gccgggctga aggccgagct cggcatcacc gccgagcagg
     4741 aggctgcgtg gcaggcgttc gtgaacagcg ccaagcagcg cgaagaggcg cgcgaggcgt
     4801 tcttcgccaa gcggcgcgaa gcggcggacg ccggcacgct gcccgagcgg cttgcgcagc
     4861 gcgacgagtt cttcaagcag catcaggccg agcggcaggc gcagactgcg gcgctgaagg
     4921 agctctacgc cgcgctctcg ccggagcaga aggcgatcgc ggatgagcgc ttcggcggct
     4981 tcggcccgcg gtatggcgcg ggctacggcc gcggctttgg tggacgttct cgctaggccg
     5041 cgaacgagcg gggcggctgt gcagccgccc cgctttgttt cctgcgcggc gcgacagctg
     5101 gctaggcgag cgcggccccg cacttcgagc agaagcggtc gcccgccgcg gcagggccgc
     5161 cgcacttgcg gcagaacctc gcgcccgcgt tgccgcccgg gcccgggcgc tcgcgccgcc
     5221 gccaccagaa caggtacacg agacccgagc cgacgaggag cagcgcgatg gcgccgctga
     5281 cgaggaacct gcggaacgcg tcgcgctctt cgctcgaagt gccggccccg ggactcgacg
     5341 cgggcgtccc ggccttgagg atttcggccg aagtgcgcgg gtccgatttg gcgtagcgga
     5401 tctcgatgga tcgggcctgt ccctgcggca cgggaccgag aagcgccgag cgatagcgca
     5461 agccgtcgat gccggtggac aggtcggcga gcgccggctg taccgcgata tcggtcgccg
     5521 cggcgggctc ctggaccacg acctgcagcg cgcccaccgc caggtctccc ggccacacgt
     5581 acttgtagct gcgcgcgggc gtaccggtga cgagcgggtc gtagaactcg acgtggaaga
     5641 aacgctccgg cgtggtgaaa cgcaacgcga tggcatcggc cgccgccgat cgatcgtgcg
     5701 cgagattcag gagcggcccc gtcttctgcg gcgcgaatgc caccgccgac ggcccgcccg
     5761 aggaggcggg gatgcgcagc gtcagcgtta cgggcaggcc gacgtcgctg gcgagctcgc
     5821 ccttcaggat caccagtgcg gccgggcggt cgtattcggg ccagatctcg acctgcaggc
     5881 tctccaggcg cggcgcaccg gccgcggcgc acgcaaggcc gggcacgagc aatagggcca
     5941 gcaacagctt gtgcagcatc gcccggaagt atcccattgc gcgcggcgcg catattgatc
     6001 acgatcaagc ctgtgagccg gatcctgttc ctgtattcga ccgtagacgg gcatacgctc
     6061 aagatctgcc agcggctgca gcagatcgtg gaaggggcgg gacaccgcgc ggcgctcgaa
     6121 gtcctcgaag cgcacgcgga catggacctc gagcccttcg accggatcgt cgtcggcgcg
     6181 agcatccgct acggcaagca ccgccccgag gtcgccgagt tcatgcgccg gcggcgccgc
     6241 atcctcgagg ccaagccgag cgccttcttc tcggtcaaca tcgtggcccg caaggccggg
     6301 aaggacacgc cgcagaccaa tccctacgtg gtgaagttcc tgcgccagat cggctggcga
     6361 cccgcgctgg tcgcggtgtt cgcggggaag ctcgactatc ccaggtacgg cttctgggac
     6421 cgccagatca tccgcttcat catgctgctg acccgcgggc ctaccgaccc gcgcgccgtg
     6481 gtggagttca cggactgggc ggaggtcgat gccttcgggc gccggctcgc cgcgatctag
     6541 gcgccgcacc agtcggcgat gatcttctcg tagatctcca ccgactggcg gatgcgctcc
     6601 atgctgcagt actcgtcggt ctggtgcgcc atctgcggct cgcccggccc gagcacgacg
     6661 gtgggcacgt cgccgtagac cttgcgcagg ttcgcggcgt ccgtcatgta ggtcgcggtg
     6721 cgcacctccg gcggcttgcc gaggaacggc gtgcagatct cgaacacgcg ctgcatccac
     6781 tcggcctccg gcgcggtcca caccgcgggc aggtccgaga aggtgtcgat cgccacgtcc
     6841 tcgcccatcg cggcgcgcag gcgcgcgagc agcgcggcgt ggtccatgcc gggcagcgtg
     6901 cgcacatcga cgccgatgcg cgcggcatcc ggcaccaggt tgacgcccga gccgccctcg
     6961 aaggtgccga cgctcaacgt cgccgcgccc atcaccgcat gcggcggctg gcgaaaatcg
     7021 aacgccgcga gcccgctcac cacgcgcgcc gccttgtaga tcgcgttcac gccgagctcg
     7081 ggcatcgagc cgtgcgccga gacgccgcgg aaatcgg
//
LOCUS       DBNHKE010000089        14828 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1019764, whole genome shotgun sequence.
ACCESSION   DBNHKE010000089 DBNHKE010000000
VERSION     DBNHKE010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1019764"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="AC16O9_05570"
     CDS             <1..378
                     /locus_tag="AC16O9_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR6516119.1"
                     /translation="EILDAGVQVMAHPPGKRNTSIHLLSGGEKALTAIALVFSMFQLN
                     PAPFCLLDEVDAPLDDSNTERFCNLVRKMSAQTQFLFISHNKITMEMAAQLIGVTMPE
                     SGVSRVVAVDIDEALRIREELAA"
     gene            407..1330
                     /locus_tag="AC16O9_05575"
     CDS             407..1330
                     /locus_tag="AC16O9_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HIR6516120.1"
                     /translation="MSDLQIGLVAVGVLVVAGIFAYNALQERRARRAAERAFRSGHAD
                     VLMDGGGQRVEPRFDGPSRRAPPSETAALPDRRLDYIVELELRQAASAAEVAAHWAPF
                     EHRFAGRALLAASPDRAAWRPLTAAGSCVAVLAALQLASRSGPVSEAQLIEFRGAVDT
                     LAAQIGAGVSAPEIKQAVDAARELDALCADADIQVVLHVVPQPGGEFAGAAAPQAPGT
                     FGVARRDDGGYVLSIDVPRSSEVRRTYDAMVVYARDLCATLGGMIVDDNARVLDEHAL
                     AAIGAQLETVRATLEARGIEPGSPAALRLFS"
     gene            1335..3407
                     /gene="ligA"
                     /locus_tag="AC16O9_05580"
     CDS             1335..3407
                     /gene="ligA"
                     /locus_tag="AC16O9_05580"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6516121.1"
                     /translation="MAGTASAHHRAAALRRELERHNRLYYVEDAPEISDAAYDKLFAE
                     LQQLEAEHPELASTDSPTQRVGAPPLAAFAEVQHRTPMLSLANAFSEDEVRAFDRRLR
                     EALRADELEYTAEPKFDGLAVNLAYRDGVFLQGATRGDGATGEDVTPNLRTVGAIPLR
                     LTDGVTLEVRGEVLMFRRDFAALNERQRAAGHKEFVNPRNAAAGSLRQLDSRVTAQRT
                     LRFFAYGVGQAPGARWRTQAEILERLAALGFPVVRDRAVVRGVDGMLRYYAKLGEMRS
                     QLPYAIDGVVYKVNRLDWQERLGYVARAPRFALAHKYAAEEQETEVLGIDVQVGRTGV
                     LTPVARLQPVFVGGVTVTNATLHNEDELRRKDVRVGDTVIVRRAGDVIPEVVAVRPDK
                     RPARTPEFRMPEQCPVCGSAVARLPGEAATRCTGGLYCRAQRVQALLHFKARRAMDIE
                     GLGDKLAEQLVELEMVASPADLYTLDADALAGLERMGEKSARNVLESIERSRDATLGR
                     FVFALGIPGVGEEVAKILARHFGSLERLLEADWERLADDKEGVRKDNAKRKRRGEPPA
                     PVPLEGIGPEIMESVRRFVQEAHNRDVIRRLTDRRTGVRLQDEPGVTPARAAKTFVLT
                     GALPGMTRDEAKSLIESKGHKVASAVSRNTDYVVAGSDAGSKLERARELGVAVLDEQG
                     LKDTLEKL"
     gene            3413..4288
                     /gene="galU"
                     /locus_tag="AC16O9_05585"
     CDS             3413..4288
                     /gene="galU"
                     /locus_tag="AC16O9_05585"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR6516122.1"
                     /translation="MPPPLRKAVFPVAGFGSRFLPATKASPKEMLPIVDKPLIQYAVE
                     EAAAAGITDMIFITGRHKRAIEDHFDKATEIESDLEAKGKRALLEVVQGVLPPGCRCI
                     YTRQPEPLGLGHAVLCAQPVVGNEPFAVLLADDLIDAQPSVTAQMAQHFSVQGCSILG
                     VMEVARERTASYGIVSTEQAGAALSAIRSMVEKPKPAEAPSTLAVVGRYVLTPRIFDM
                     LAAVQPGSGGEIQLTDGIAALLHEERVMAYRFAGRRFDCGSKLGYLQATVELGLKHGE
                     VGAGFARYLAERARA"
     gene            4281..4829
                     /locus_tag="AC16O9_05590"
     CDS             4281..4829
                     /locus_tag="AC16O9_05590"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HIR6516123.1"
                     /translation="MPDLRNAWQFLEESDLVCSEQEIQAALARLAAQIDARLRDAYPV
                     VLVVMGGAVVFAGQLLPRLRFPLDLDYVHATRYGAATSGGGIAWRVSPPEDVRGRTVL
                     LLDDILDHGKTLGAIRDQLLAQGAAEVLSAVLVEKELTVPKPIRADFVGVRIPDRFVF
                     GCGMDAKGFWRNLPEIRAMRAP"
     gene            4826..5566
                     /locus_tag="AC16O9_05595"
     CDS             4826..5566
                     /locus_tag="AC16O9_05595"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR6516124.1"
                     /translation="MTLAVIGGSGLSQLASLEVTRRKMVRTPYGEPSGALTFGAIRGH
                     GMVFLARHGYGHTIAPHEVNYRANLWALKEESVSGVVSVASVGAIRRDVAPGALLVPD
                     QIIDYTWGRRATFFEGAGAPVTHIDFTEPYTPSLRKRILEAARACGERVLDGGTYAAT
                     QGPRLESAAEINRLERDGADVVGMTGMPEAALARELGLEYAAIAVSANYAAGRGESAH
                     AIPMERIEAVLAEAMGRVRRIIERLVGG"
     gene            5563..6096
                     /gene="def"
                     /locus_tag="AC16O9_05600"
     CDS             5563..6096
                     /gene="def"
                     /locus_tag="AC16O9_05600"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6516125.1"
                     /translation="MIREVLRMGDARLLQKAQPVGQFGTPELDALLTDMRDTMAHYSG
                     AGLAAPQIGVGLRVVIFGVHANPRYPHVEEVPDTVLINPVLEPLAADKEEDWEGCLSV
                     PGMRGVVPRFRHLRYAGFDERGRRFERVVEGFHARVVQHECDHLDGVLYPMRIRDFRK
                     FGFNEALFPGEALPLEE"
     gene            complement(6099..8657)
                     /locus_tag="AC16O9_05605"
     CDS             complement(6099..8657)
                     /locus_tag="AC16O9_05605"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR6516126.1"
                     /translation="MPAPAEQWRAGLVAGRTALRESYLRAAAPQRQLRAQSRLIDRTL
                     RGLWRELRPASPAALVATGGYGRGELFPHSDIDVLILLGAQPGPEERSSLERLVGMFW
                     DVGLEVAHSVRTVQECVEVAAADITVQTTLLEARFLAGSRGLYRELTAAVAAALDTQA
                     FFKDKTLEQEQRHAKHQDTAYALEPNLKEAPGGLRDLQTIQWIARACGLGRHWRDLHR
                     HGLLLPAEAVQLARHEKLLHDLRIRLHYLAGRREDRIVFDYQTPLAEQCGYRGSPTRR
                     ASEQLMQRYYTTAKAGTQLNTILLHNLEARLIGESDATPLALNERFRVRGERLEACAE
                     DLFEREPRAILESFVLLQQHQELRGMTAATLRALWRARPRVNARLRRDPVARLLFLHF
                     LQQPRGIVHEFRRMNQYGILGRYLPEFGRIVGQMQHDLFHVYTVDQHILMVVRNLRRF
                     TMPEFAHEFPLCSQLMNGFERRWLLYVAALYHDIAKGRGGDHSELGMRDVRAFCRRHG
                     LSREDGELVAFLVEHHLSLSGTAQKQDVHDPEVVRAFAALVGSERRLVALYLLTVADI
                     RGTSPKVWNAWKAKLLEDLFRATRRVLSGDAPALDAALAEKQEEAARLLRLYALSDEV
                     KERFWAQLDTPYFLRHDAQEIAWQTRNLHYRVDATTPVVKARLAPIGEGVQVMIYVPD
                     REGLFARICGYFDRSGFNIAEAKVHTTRHGYALDTFLVMGQGLAAHYRDMLLQIEKEL
                     AQEVDSHEALPPPRGGRLSRRARHFPVSPAVDLRPDERGAYQVLSIVASDRPGLLYAV
                     ARTLAQHGVSVHSAKINTLGDRAEDVFLVSGEALQQPRQALQLEQQLLRELQIK"
     gene            complement(8668..9459)
                     /gene="map"
                     /locus_tag="AC16O9_05610"
     CDS             complement(8668..9459)
                     /gene="map"
                     /locus_tag="AC16O9_05610"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR6516127.1"
                     /translation="MAAVLKTPEEAAKMRVAGRLAAEVLDYITPHVRPGVSTSALNDL
                     CHAYMVEVQGTTPAPLNYAPPGYKPFPKSICTSVNHQVCHGVPGERVLKPGDILNIDI
                     TVIKDGFHGDSSRMFYVGEPSIQARRLVEVTYECMWAGIRQVRAGSHLGDIGAAIQAH
                     AEKHGYSVVREFCGHGIGRRFHEEPQVLHYGKPGTGLRLEAGMTFTVEPMINAGKAGI
                     RELADGWTIVTKDHSLSAQWEHTVLVTQDGHEVLTTSAGTPPAPA"
     gene            9605..10351
                     /gene="rpsB"
                     /locus_tag="AC16O9_05615"
     CDS             9605..10351
                     /gene="rpsB"
                     /locus_tag="AC16O9_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR6516128.1"
                     /translation="MSATMRQMLEAGVHFGHQTRYWNPKMAPYIFGHRNKIHIINLEK
                     TLVLYQEALKYVRQLAANRGSILMVGTKRQAREIVREEAQRCGMPFVDHRWLGGMLTN
                     FKTVKQSIKRLKEMEQMAEDGTFDGMAKKEALGLQRELAKLQRSLGGIKDLNSLPDAL
                     FVIDVGYQKGAIAEAVKLGIPVVGVVDTNHSPEGISYVIPGNDDSSRAIRLYARGIAD
                     AVLDGRSNAVEEVAAASRDEFVEVDDEGGA"
     gene            10418..11281
                     /gene="tsf"
                     /locus_tag="AC16O9_05620"
     CDS             10418..11281
                     /gene="tsf"
                     /locus_tag="AC16O9_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR6516129.1"
                     /translation="MAEISASLVKELRELTGLGMMECKKALAEAGGDLKKAEELLRIK
                     SGAKASKAAGRVAAEGVVGAWLAPDAKLGALVELNCETDFVAKNEDFLAFAREVAEAI
                     ARDNPADVQGLAKFESRRQALVQKIGENISIRRFARLQAQGRLALYVHGAKIGVLVDA
                     EGPEDLGKDLAMHVAAFKPQYLSKAEVPADIVARERDIATERAKETGKGKPPEIVAKM
                     VEGAVAKFLNENTLLGQPFVKDDKLTVERVLAAKKAKLHGYRVLVVGEGIERKSSDFA
                     AEVMATAGAGR"
     gene            11294..12040
                     /gene="pyrH"
                     /locus_tag="AC16O9_05625"
     CDS             11294..12040
                     /gene="pyrH"
                     /locus_tag="AC16O9_05625"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR6516130.1"
                     /translation="MKPREAAIDAKYTAKYKRILLKLSGEALMGDGQYGINRQTIEAI
                     VQEIAAVARLGVELGVVIGGGNIFRGVAPGAAGMDRATADYMGMLATVMNALALQDAM
                     RRAGLDCRVQSALNIEQVVEPYIRGKAIRYLEEGRIVVFAAGTGNPFFTTDTAAALRA
                     SEVGAEIVLKATKVDGVYTADPKKDRAATRYARLSFDEAIQRNLRVMDATALALCRDQ
                     KLPVNVFSIFKKDALLRVVAGEDEGTLVHV"
     gene            12052..12609
                     /gene="frr"
                     /locus_tag="AC16O9_05630"
     CDS             12052..12609
                     /gene="frr"
                     /locus_tag="AC16O9_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR6516131.1"
                     /translation="MLAELKKTTEQKMHKSVEAFKSDLSKVRTGRAHTGLLDHITVDY
                     YGTPTPLNQVAKVTLLDARTIGVAPFEKKMLQPVEKAIRDSDLGLNPASMGETVRVPM
                     PALTEERRKELIKVVRHEAENARVAVRNLRRDANHHLKEALKKKEVSENDERRAQDDV
                     QKLTDRAIAEIDKMLQQKEAELLAV"
     gene            12616..13404
                     /gene="uppS"
                     /locus_tag="AC16O9_05635"
     CDS             12616..13404
                     /gene="uppS"
                     /locus_tag="AC16O9_05635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR6516132.1"
                     /translation="MGSDSSTQAVPAHGAVPRHVAVIMDGNGRWARQRHLPRIAGHKR
                     GVEAVRNTVQACASRGVQYLTLFAFSSENWRRPAEEVALLMQLFQVALTSEVEKLHRN
                     GIRLRVVGDLSRFDGRMRALIERAEHLTAANNRLTLTVAANYGGRWDILQALGRLLRE
                     RPDLAPGPIEEGHIAPYLAMSYAPEPDLFIRTGGEQRVSNFLLWQLAYTELYFTDTLW
                     PDFDASALDVAIASYAQRERRFGRTSEQLEDAKRAQSPATGALA"
     gene            13391..14215
                     /locus_tag="AC16O9_05640"
     CDS             13391..14215
                     /locus_tag="AC16O9_05640"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR6516133.1"
                     /translation="MRSPEGLLRRILTAAVLLAAFLAALLLLERRLFALLVALVLVLA
                     GVEWGRLAGLGSRAALAYGIAIGVLGGAVAWLPGTGDAWRDTLLAAGTAFWLMLVPAW
                     LYRGLARSSRTALALAGFLALALAGFAALSLPPSYLLLVLGLAWIADTAAYFAGNAFG
                     RRRLAPSISPGKTWEGVAGAVVACLIYAIICALPGAPFGPFVQGATWIPYLAGAALLC
                     GVSIVGDLFESGLKRRAGAKDSGGLLPGHGGVLDRIDSIMPTLPIAAMLIDLMTRT"
     gene            14212..>14828
                     /locus_tag="AC16O9_05645"
     CDS             14212..>14828
                     /locus_tag="AC16O9_05645"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858811.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR6516134.1"
                     /translation="MTPEHGRADAQRPGSLVILGATGSIGSSTLDVVARHPRRYRVHA
                     LTAHASAQGLLALCQRHRPRYAVLSGCAEDAALRRSFADVGCELRFGPAALADLAAAP
                     EGDTVMAAIVGAAGLPATLAAARAGKRVLLANKEALVMAGPLLTRAARDAGAVLLPID
                     SEHNAVFQCLPPEGAGMRGVRRIQLTASGGPFRATPAERLAGVTP"
BASE COUNT         2339 a         4966 c         5294 g         2229 t
ORIGIN      
        1 gagatcctcg acgcgggcgt acaggtgatg gcgcacccac cgggcaagcg caacaccagc
       61 atccacctgc tgtcgggggg cgagaaggcg ctgaccgcga tcgcgctggt gttctccatg
      121 ttccagctca accctgcgcc gttctgcctg ctcgacgagg tcgacgcgcc gctcgacgac
      181 agcaacaccg agcgattctg caacctggtg cgcaagatgt cggcgcagac gcagttcctg
      241 ttcatcagcc acaacaagat caccatggag atggcggcgc agctgatcgg cgtcaccatg
      301 cccgagtcgg gcgtgtcgcg cgtggtcgcc gtggacatcg acgaggcgct gcgcatccgg
      361 gaggagctcg ccgcctgatc gcggcgggcg gacctgcgcc ccggcgatga gcgacctgca
      421 gatcgggctg gtggcggtgg gcgtgctggt ggtcgcgggc atcttcgcct acaacgcgct
      481 ccaggaacga cgcgcgcggc gcgctgcgga acgagctttc cgttccgggc atgccgacgt
      541 gctgatggac ggtgggggac agcgcgtcga gccccgtttc gatgggcctt cgcgccgggc
      601 gccgccctcg gagacggcgg ccctgcccga tcggcgcctg gattacatcg tcgagctgga
      661 gctgcgccag gccgcatcgg ccgccgaggt cgccgcgcac tgggcgccct tcgagcaccg
      721 ctttgcgggc cgagcgctcc tcgccgccag ccctgatcgc gctgcctggc ggccgctgac
      781 cgcggcgggc agctgcgtcg cggtgctcgc ggcgctgcag ctggcgagcc gctccgggcc
      841 ggtcagcgaa gcgcagctca tcgagtttcg aggtgccgtg gatacgctcg ccgcgcagat
      901 cggggcgggc gtatcggccc cggagatcaa gcaggccgtc gacgcggcgc gcgagctcga
      961 cgcgctgtgc gcggatgccg acatccaggt agtgctgcac gtcgtgcccc agcccggcgg
     1021 cgaatttgca ggcgcggcgg cgccgcaggc gccgggcacc ttcggcgtgg cgcggcgcga
     1081 cgacggcggc tacgtactgt cgatcgacgt gccgcgcagc tccgaggtgc gccgaaccta
     1141 tgacgcgatg gtggtctacg cgcgcgacct gtgcgcgacc ctgggcggca tgatcgtcga
     1201 cgacaatgcg cgggtcctgg acgagcacgc gctcgccgcc atcggcgcgc agctcgagac
     1261 cgtacgcgcc acgctcgagg cgcgcggcat cgagcctggc agccccgcgg cgctgcgact
     1321 gttctcctaa aggcatggcg ggcacggcct ccgcgcatca tcgggccgcc gcactgcgcc
     1381 gggagctcga gcgccacaat cgcctgtatt acgtggagga cgcgcccgag atctcggacg
     1441 ccgcctacga caagctgttc gccgagttgc agcagctgga agccgagcat ccggagctcg
     1501 cttcgaccga ctccccaacc cagcgcgtcg gtgcgccgcc cctcgctgca ttcgccgagg
     1561 tgcagcatcg cacgccgatg ctgtctctgg cgaacgcgtt ctccgaggac gaggtgcgcg
     1621 cgttcgaccg ccggctgcgc gaggcactgc gggcggacga gctcgagtac accgccgagc
     1681 ccaagttcga cggtctggcg gtaaacctcg cctaccgcga cggcgtattc ctgcagggcg
     1741 cgacgcgcgg cgacggcgcc accggcgagg acgtgacgcc gaacctgcgc acggtgggcg
     1801 cgattccgtt gcgattgacc gacggcgtga cgctcgaggt gcgcggcgag gtgctcatgt
     1861 tccggcgcga cttcgcggcg ctcaatgagc gccagcgtgc cgccggccac aaggaattcg
     1921 tcaacccgcg caacgccgcc gccggcagcc tgcgccagct ggattcccgc gttacggcac
     1981 agcgcacgct gcgcttcttt gcctacggcg tgggacaggc gcctggcgcg cgctggcgca
     2041 cccaggccga gatcctcgag cggctcgccg cactcggctt tcccgtggtc cgcgaccgcg
     2101 cggtggtgcg cggcgtcgac ggcatgctga ggtactacgc gaagctgggc gagatgcgct
     2161 cgcagctgcc ctacgcgatc gacggcgtgg tctacaaggt gaatcgcctc gattggcagg
     2221 agcgcctcgg ctacgtcgcg cgcgcgccgc gcttcgccct ggcccacaag tacgccgccg
     2281 aggagcagga gactgaagtg ctcggcatcg acgtgcaggt cgggcgcacc ggcgtgctca
     2341 cgccggtggc gcgcctgcag ccggtgttcg tcggcggcgt taccgtgacc aacgccaccc
     2401 ttcacaacga ggacgagctg cggcgcaagg acgtgcgagt cggcgacacg gtgatcgtgc
     2461 gccgcgccgg cgacgtgatt cccgaggtgg tcgcggtgcg gcccgacaag cgccctgcgc
     2521 gcacgcccga gttccgcatg ccggaacaat gcccggtgtg cggctcggcg gtcgcgcggc
     2581 tgcccggcga ggcggccacg cggtgcaccg gcggtctgta ctgccgggcg cagcgggtgc
     2641 aggcgctgct gcatttcaag gcgcgccgcg ccatggacat cgagggtttg ggcgacaagc
     2701 tcgccgagca gctggtcgag ctggaaatgg tggcgagccc ggccgacctg tatacgctgg
     2761 atgccgacgc gctggcgggg ctcgagcgca tgggcgagaa gtcggcgcgc aacgtgctcg
     2821 agagcatcga acgctcgcgc gacgcgacgc tcgggcgctt cgtgttcgcc ctcggcattc
     2881 ccggggtcgg ggaggaagtg gcgaagatcc tggcacgcca cttcggctcg ctcgagcggc
     2941 tgctcgaagc ggactgggag cggctcgccg acgacaagga gggggtgcgc aaggacaacg
     3001 ccaagcgcaa gcgccggggc gagcccccgg cgccggtgcc gctcgagggc atcggcccgg
     3061 agatcatgga aagcgtgcgg cgcttcgtgc aggaggcaca caatcgcgat gtgatccgcc
     3121 ggctcaccga tcgccgcacg ggcgtgcgcc tgcaggacga gcccggcgtg acgccggcgc
     3181 gcgcggcgaa gaccttcgtg ctcaccggcg cgctgcccgg catgacgcgc gacgaggcaa
     3241 aatcgctgat agagtccaag gggcacaagg tcgcgagcgc cgtgtcgcga aacaccgatt
     3301 acgtggtcgc cggcagcgac gccggcagca agctggagcg ggcgcgagag ctcggcgtcg
     3361 cggtgttgga tgaacaagga ctgaaggaca ccctggagaa gctctgacac gcatgccgcc
     3421 ccctttgcgc aaggccgtgt tccccgtcgc cggcttcggc agccggttcc ttcccgccac
     3481 caaggcgagc ccgaaggaga tgctgccgat cgtcgacaag ccgctcatcc agtacgcggt
     3541 cgaagaagcc gcggccgccg ggatcaccga catgatcttc atcaccggcc gccacaagcg
     3601 cgccatcgag gatcatttcg acaaggccac cgagatcgag tcggatctgg aggcgaaggg
     3661 caagcgcgcg ctgctcgagg tggtgcaggg cgtgctgccg cccggctgcc gctgcatcta
     3721 cacgcgccag cccgagccgc tcggcctggg acacgcggtg ctctgcgcgc agccggtggt
     3781 cggcaatgag ccgttcgccg tgctgctcgc cgacgacctg atcgacgcgc agccgagcgt
     3841 gaccgcgcag atggcgcagc acttcagcgt gcaaggctgc tcgatcctgg gcgtgatgga
     3901 ggtggcgcgc gagcggacgg cgagctacgg catcgtcagc accgagcagg ccggcgccgc
     3961 gctgagcgcg atccggagca tggtggagaa gcccaagccc gccgaggcgc cttcgacgct
     4021 ggcggtcgtc gggcgttatg tcctcacgcc gcgcattttc gacatgctcg ccgcggtgca
     4081 gcccggcagc ggcggtgaga tccagctcac cgacggcatc gcggcgctcc tgcatgaaga
     4141 gcgcgtcatg gcctaccgct tcgccggccg gcgcttcgat tgcggctcga agctcgggta
     4201 cctgcaggcc acggtggagc tgggcctcaa gcacggcgaa gtcggggccg ggttcgcgcg
     4261 ctatctggcg gagcgcgccc gtgcctgatc tgcgcaacgc gtggcagttc ctcgaggaat
     4321 cggacctcgt gtgcagcgag caggagatcc aggcggcgct cgcgcggctc gccgcgcaga
     4381 tcgatgcccg cctgcgtgac gcgtaccccg tcgtgctggt ggtgatgggc ggcgcggtgg
     4441 tgtttgccgg gcagctgctg ccgcgcctgc ggtttccgct cgaccttgat tacgtccatg
     4501 cgacgcgcta tggcgccgcg acgagcggcg gcggcattgc ctggcgcgtc agcccgccgg
     4561 aggacgtgcg cggccgcacc gtgctgctgc tcgacgacat cctcgaccac ggcaagacgc
     4621 tcggcgcgat ccgtgatcaa ctgctggcgc agggcgcagc cgaggtgctg agcgccgtgc
     4681 tcgtggaaaa ggagctcacc gtgcccaagc cgatccgcgc cgacttcgtc ggcgtgcgca
     4741 ttcccgaccg cttcgtcttc ggctgcggca tggacgccaa ggggttctgg cgcaacctgc
     4801 ccgagatccg cgccatgagg gcgccgtgac cctcgcggtc atcggcggca gcggcctgtc
     4861 gcagctcgcg agcctggagg tgacgcggcg caagatggtg cgcacgccct acggcgagcc
     4921 ctcgggcgca ctgaccttcg gcgcgattcg cggccacggc atggtgttcc ttgcgcggca
     4981 cggctacggc cacaccatcg cgccgcacga ggtgaactac cgcgccaacc tgtgggcgct
     5041 gaaggaggaa agcgtctccg gcgtcgtctc cgtcgcctcg gtgggcgcca tccggcgcga
     5101 cgtggcgccg ggcgcgctgc tggtccccga ccagatcatc gactacacct ggggccgccg
     5161 cgccaccttc tttgaaggcg cgggcgcgcc ggtcacgcac atcgatttca ccgagccgta
     5221 tacgccttcg ctgcgcaagc gcatcctcga ggcggcgcgc gcctgcggcg agcgcgtgct
     5281 cgacggtggc acctacgcgg cgacgcaggg gccgcggctg gaaagcgcgg ccgagatcaa
     5341 ccggctcgag cgcgacggcg cggacgtggt cggcatgacc ggcatgccgg aagcagctct
     5401 cgcgcgcgag ctgggactgg agtacgcggc gatcgcggtc agcgcgaatt acgccgccgg
     5461 ccgcggcgag agcgcgcacg ccattccgat ggagcgcatc gaggcggtgc tggcggaggc
     5521 catgggtcgg gtgcgccgca tcatcgagcg gctggtgggc ggatgatccg cgaggttctg
     5581 cgcatgggcg acgcgcgcct gctgcaaaag gcgcagccgg tggggcagtt cggcacgccc
     5641 gagctcgacg ctttgcttac cgacatgcgc gatacgatgg cgcactacag cggcgcgggg
     5701 ctggcggcac cgcaaatcgg ggtgggtttg cgcgtggtga tcttcggcgt gcacgcgaat
     5761 ccgcgctatc cccacgtgga ggaagtgccc gacaccgtgc tcatcaaccc ggtgctcgag
     5821 ccgctggccg ccgacaagga ggaggactgg gaaggctgcc tctcggtgcc gggcatgcgg
     5881 ggcgtggtgc cgcgctttcg ccacctgcgc tatgcgggct tcgacgagcg ggggcggcgt
     5941 ttcgagcggg tggtggaggg gtttcacgca cgggtggtgc agcacgagtg cgaccacctc
     6001 gacggcgtgc tctacccgat gcgcatccgg gacttccgta agttcggctt caacgaggcc
     6061 ttgttcccgg gcgaggcgct gccgctggag gagtgaggct acttgatctg cagctcgcgc
     6121 agcagctgct gctcgagctg caacgcctgc cgcggctgct gcagggcttc gccggatacc
     6181 aggaacacgt cctcggcgcg gtcgccgagc gtattgatct tggccgaatg cacgctcacg
     6241 ccgtgctgcg ccagcgttcg cgccaccgcg tacagcaggc ccgggcggtc gctggcgacg
     6301 atcgacagga cctgataggc gccgcgctcg tcggggcgca ggtccaccgc cggggacacc
     6361 gggaagtgcc gggcgcgccg tgacagccgg ccgccacggg gcggcggcag cgcctcgtgc
     6421 gaatcgacct cttgcgccag ctccttctcg atctgcagca gcatgtcgcg gtaatgcgcg
     6481 gcgaggccct ggcccatgac caggaacgtg tccagcgcgt agccgtggcg cgtggtgtgc
     6541 accttggcct cggcgatgtt gaagccgctg cggtcgaaat agccgcagat gcgggcgaac
     6601 agcccttccc ggtcgggaac atagatcatg acctgcacgc cctcgccgat cggtgccagc
     6661 cgcgccttca ccaccggcgt cgtggcgtct acccggtagt gcagattgcg tgtctgccag
     6721 gcgatctcct gcgcgtcgtg gcgcaggaag tagggcgtat cgagttgcgc ccagaagcgc
     6781 tccttgacct cgtccgagag cgcgtacagg cgcagcagtc gtgccgcctc ttcctgcttt
     6841 tccgccagcg ccgcgtcgag cgccggcgca tcgccggaca gcacgcggcg cgtggcgcgg
     6901 aacagatcct cgagcagctt cgccttccag gcgttccaca ccttcgggct ggtgccgcgg
     6961 atgtcggcga cggtaagcag gtacagcgcc accagccgcc gctcgctgcc caccagcgcg
     7021 gcaaacgcgc gcaccacctc cggatcgtgt acgtcctgtt tttgcgcggt acccgacagg
     7081 ctcaggtggt gctcgacgag aaaggcgacg agctcgccat cctcccgtga cagtccgtgg
     7141 cgccggcaga acgcccgcac gtcgcgcatg cccagctcgg agtggtcgcc gccgcggccc
     7201 ttggcgatgt cgtggtagag cgccgccacg tacagcagcc agcgccgctc gaagccgttc
     7261 atcagctggc tgcacagcgg aaactcgtgc gcgaactcgg gcatggtgaa acgacgcagg
     7321 ttgcgcacca ccatcaggat gtgctggtct acggtgtaaa cgtggaacag gtcgtgctgc
     7381 atctggccga caatgcggcc gaactcgggc aggtaacgcc cgaggatgcc gtactggttc
     7441 atgcgccgga attcgtgcac gatgccccgc ggctgctgca ggaaatgcag gaacaacagc
     7501 cgtgccaccg gatcgcgtcg cagccgggcg ttgacacgcg gccgcgcgcg ccacagcgcg
     7561 cgcagcgtgg ccgcggtcat gccgcgcagc tcctggtgct gctgcagcag cacgaaactc
     7621 tcgagaatcg cgcgcggctc gcgctcgaac agatcctcgg cgcaggcctc gagccgctcg
     7681 ccgcgcaccc ggaagcgctc gttgagggcg aggggcgtgg cgtcggactc accgatcagg
     7741 cgcgcctcca ggttgtgcag caggatggtg ttcaactgcg tccccgcctt ggcagtggtg
     7801 tagtagcgct gcatgagctg ctcgctcgcg cggcgggtcg gcgagccgcg gtagccgcat
     7861 tgctcggcga gcggcgtctg gtagtcgaag acgatgcggt cctcgcgccg ccccgccaga
     7921 tagtgcaggc gaatgcgcaa gtcgtgcagc agcttctcgt gccgtgccag ttgcaccgcc
     7981 tcggcgggca gcagcaggcc gtgccggtgc agatcgcgcc aatgccgacc gaggccgcag
     8041 gcacgagcga tccactggat ggtctgcagg tcgcgcaggc cgccgggcgc ctccttcaga
     8101 ttcggctcca gcgcgtaggc ggtgtcctgg tgcttggcgt gccgctgctc ctgctcgagc
     8161 gtcttgtcct tgaagaaagc ctgcgtatcc aacgcggccg ccacagcagc ggttagctcg
     8221 cggtacaggc cgcggctgcc ggcgagaaag cgcgcttcga gcagagtagt ctgcaccgtg
     8281 atatccgcgg cggcgacctc gacgcactcc tgcacggtgc gcacgctatg cgcgacttcc
     8341 aggccgacgt cccagaacat gccgaccagg cgctctaggc tgctgcgctc ctcgggcccg
     8401 ggctgcgcgc ccagcaggat caggacgtcg atgtccgagt gcggaaagag ctcaccgcgg
     8461 ccgtagccgc cggtcgccac cagagcggcg ggcgacgccg gacgcagctc gcgccacagg
     8521 ccgcgcagcg tgcggtcgat cagccggctc tgggcgcgca gctggcgctg cggagcggct
     8581 gcgcgcaggt aggactcgcg cagcgcggtg cggcccgcca ccagccctgc gcgccactgt
     8641 tccgccggcg caggcaccgc gctcacgtca agcgggggca ggcggcgtgc cggcggaagt
     8701 cgtcagcacc tcgtggccgt cctgcgtgac gaggaccgtg tgctcccact gtgccgagag
     8761 gctatggtcc ttggtcacga tggtccagcc atcggccagc tcacggatcc cggccttccc
     8821 ggcgttgatc atcggctcga cggtgaacgt catgcccgct tccagccgca aaccggtgcc
     8881 cggctttccg tagtgcagga cctgcggctc ctcgtgaaag cgccggccta tgccgtggcc
     8941 gcagaactcc cgcaccaccg aatagccgtg tttctcggcg tgcgcctgga tcgccgcgcc
     9001 gatgtcgccg agatgagagc cggcgcgtac ctggcggatg cccgcccaca tgcactcgta
     9061 ggtcacctcc accagccggc gcgcctggat gctcggttcg ccgacgtaga acatgcggct
     9121 cgaatcgccg tgaaagccat ccttgatcac cgtgatatcg atgttgagga tgtcgccggg
     9181 cttgagcacg cgctcgccag ggacgccatg gcacacctgg tggttgaccg acgtgcagat
     9241 cgacttgggg aacggcttgt acccgggggg cgcgtagttc agcggcgccg gcgtcgtgcc
     9301 ctgtacttcg accatgtagg cgtggcacag gtcgttcagg gcgctggtcg acacgcccgg
     9361 cctgacgtgg ggggtgatgt agtcgaggac ctcggccgcg agccggccag cgacgcgcat
     9421 cttcgcagct tcctcgggag tcttcagaac ggccgccatg gaatgagaaa aaggctgatt
     9481 atgctagaat tttaggctgg ctcgcggctt gccgcgggcc aaatttcccg tcccggccgg
     9541 ttagggtgcc cgcaagggtt ttcggttgac cggggcggca gaccataacc ctaaggagcg
     9601 cgcagtgtct gcaaccatgc gtcaaatgct ggaggccggg gtgcattttg ggcaccagac
     9661 ccggtactgg aatccgaaga tggcgccgta catcttcggc caccgcaaca agatccacat
     9721 cataaacctg gaaaagaccc tggtcctgta ccaggaggcg ctgaagtacg tgcgccagct
     9781 cgccgccaac cgcggctcga tcctgatggt cggcacaaag cgccaggcgc gcgagatcgt
     9841 gcgcgaggaa gcgcaacgct gcggcatgcc gttcgtcgac caccgctggc tcggcggcat
     9901 gctgaccaat ttcaagaccg tcaagcagtc catcaagcgc ctgaaggaaa tggagcagat
     9961 ggccgaggac gggaccttcg acggcatggc gaagaaggaa gcgctcggac tgcagcgcga
    10021 gctggccaag ctgcagcgat ccctcggcgg catcaaggac ctcaacagcc tgcccgatgc
    10081 gcttttcgtg atcgacgtcg gctaccagaa gggcgccatt gccgaggccg taaagctggg
    10141 cattcccgtg gtgggcgtcg tggacaccaa tcattcgccg gagggcatca gctacgtgat
    10201 ccccggcaac gacgattcga gccgcgccat tcgcctctac gcgcgcggca tcgccgatgc
    10261 ggtcctcgac ggccgctcga acgctgtcga ggaagtcgcc gcggcctcgc gcgacgagtt
    10321 cgtcgaggtc gacgatgagg ggggcgcctg aggccggacc gacaagcggg acccgggggc
    10381 cttcggcccc tttttttgac ttctggagca tgacgcaatg gcggaaatca gtgcaagcct
    10441 ggtgaaggag ctgcgcgagc tgactgggct cgggatgatg gaatgcaaga aagcgcttgc
    10501 cgaggcgggc ggcgacttga agaaggccga ggagttgctg cgcatcaaga gcggggccaa
    10561 ggcaagcaag gccgcggggc gggtggcggc cgagggcgtg gtaggcgcgt ggcttgcccc
    10621 ggacgcgaag ctcggtgcgc tggtggagct gaactgcgag accgatttcg tggccaagaa
    10681 cgaggacttc ctcgccttcg cgcgggaagt cgccgaggcc atcgcgcggg acaacccggc
    10741 ggatgtgcag gggctggcca agttcgaatc gcgccgccag gctttggtgc agaagatcgg
    10801 cgagaacatc tccatccggc gcttcgcgcg gctgcaggcg cagggaaggc tcgccctgta
    10861 cgtgcatggc gcgaagatcg gggtgctggt cgacgccgag gggcccgagg acctcggcaa
    10921 ggatctcgcc atgcacgtcg cggctttcaa gccgcagtac ctgtccaagg ccgaggtgcc
    10981 cgccgacatc gtggcgcgcg agcgggacat cgccacggag cgggcgaagg agacgggcaa
    11041 gggcaagccg cccgagatcg tcgccaagat ggtcgagggt gcggtggcga agtttctcaa
    11101 cgaaaacacg ctcctcgggc agccgttcgt caaagacgac aagctgacag tggaaagggt
    11161 gcttgccgcg aagaaggcga agctccacgg ctatcgcgtg ctggtggtgg gcgaagggat
    11221 cgaaaggaag agcagcgatt tcgccgcgga agtgatggcc acggcaggcg cgggacgcta
    11281 gatcgtcaag gccatgaagc cgcgcgaagc cgcaatcgac gccaagtaca ccgcgaagta
    11341 caagcgcatc ctgctgaagc tctcgggcga ggcgctgatg ggcgacggtc agtacggcat
    11401 caaccggcag accatcgagg ccatcgtgca ggaaatcgcc gcggtggcgc gcctgggcgt
    11461 cgagctcggc gtggtgatcg gcggcggcaa catcttccgc ggcgttgcgc cgggcgcggc
    11521 gggtatggac cgcgccaccg ccgactacat gggcatgctg gcgaccgtaa tgaacgcgct
    11581 cgcactgcag gacgcgatgc gccgcgccgg cctcgactgc cgcgtgcagt cggcgctcaa
    11641 catcgagcag gtggtggaac cgtatatccg cggcaaggcg atccgctacc tcgaagaggg
    11701 caggatcgtc gtgttcgcgg cgggcaccgg caatccgttt ttcaccaccg acacggccgc
    11761 cgcgctgcgc gcgagcgaag tcggtgccga gatcgtgctc aaggcgacca aggtggacgg
    11821 cgtctatacg gccgacccga agaaagaccg tgcggcgacg cgctacgcgc gcctgagctt
    11881 tgacgaggcc atccagcgga acctgagagt gatggacgcg accgcgctcg cgctgtgccg
    11941 cgaccagaag ctgccggtca acgtgttcag catcttcaag aaggatgcgc tgttacgcgt
    12001 ggtcgccggc gaggacgaag gcacgctggt gcacgtctga aggaggattt catgctggcc
    12061 gaactcaaga agactaccga gcagaagatg cacaagagcg tcgaggcgtt caagtccgac
    12121 ctgtccaagg tgcgcaccgg ccgcgcccac accgggctgc tcgaccacat cacggtggac
    12181 tattacggca cgccgacacc actgaaccag gtcgccaagg tgacgctgct cgacgcgcgc
    12241 accatcgggg tggcgccctt cgagaagaag atgctgcagc cggtggagaa ggcgatccgc
    12301 gactccgacc tcggactgaa tcccgccagc atgggtgaaa ccgtgcgcgt gccgatgccg
    12361 gcgctgaccg aggagcggcg caaggagctg atcaaggtgg tgcgccacga agccgagaac
    12421 gcgcgcgttg cggtgcgtaa cctgcgccgt gacgccaacc accacctcaa ggaggcgctg
    12481 aagaaaaagg aggtctcgga gaacgacgag cggcgcgcgc aggacgacgt gcagaagctc
    12541 acggatcgcg ccatcgccga gatcgacaag atgctgcagc agaaggaagc ggaactcctg
    12601 gcggtctgag acaacatggg ctccgacagt tccacgcagg cagttcccgc gcacggcgcc
    12661 gtgccgcggc acgtggccgt gatcatggac ggcaacggcc gctgggcccg gcagaggcac
    12721 ctgccgcgaa tcgccggcca caagcgcggc gtggaggcgg tgcgtaacac cgtgcaagcc
    12781 tgcgcctcgc gcggggtgca gtaccttacg ctgttcgcgt tcagctcgga gaactggcgg
    12841 cgaccggccg aagaggtggc gctgctgatg cagcttttcc aggtcgccct gaccagcgaa
    12901 gtggagaagc tgcaccgcaa cggcatccgc ctgcgcgtgg tcggtgacct gtcgcgcttc
    12961 gacggccgca tgcgcgcgct gatcgagcgg gccgagcacc tgaccgccgc caacaaccgc
    13021 ctgaccctga ccgtggcggc caactacggc ggccgctggg acattctcca ggcgctcggc
    13081 cgattgctgc gggagcgccc ggaccttgca ccggggccga tcgaggaagg gcacatcgcg
    13141 ccgtatcttg cgatgagcta cgcgccggaa ccggacctgt tcattcgaac cggcggcgag
    13201 cagcgcgtga gcaacttcct gctctggcag ctcgcctaca ccgaactgta tttcacggac
    13261 acgctgtggc ccgatttcga cgcctctgcg ctggatgtgg cgatcgcctc ctacgcgcag
    13321 cgcgagcgcc gcttcggccg caccagcgag cagctcgagg acgctaagcg cgcccagtcc
    13381 ccggcgaccg gtgcgctcgc ctgaaggact gctgcggcgc atcctgacgg cggcagtcct
    13441 gctggcagcc ttcctcgccg cactgctgct gctcgagcgg aggctgttcg cgctcctcgt
    13501 cgcgctggtg ctggtgctcg ccggcgtgga gtggggtcgt ctcgcgggcc tcggctcccg
    13561 tgccgcactc gcctacggca tcgccatcgg agtgctgggc ggcgcggtgg cgtggctccc
    13621 tggcacaggc gacgcgtggc gcgacaccct gcttgctgcc gggaccgcat tctggctgat
    13681 gctggtcccg gcgtggctgt accgcggtct cgcacgctcg agccgcaccg cgctcgcgct
    13741 cgctggcttc ctggcgctgg ctttggcggg cttcgcggcg ctatcgctgc cgccgagcta
    13801 tctgctgctc gttctggggc tggcctggat cgccgatacc gcagcttact ttgcgggcaa
    13861 cgccttcggg cgacggcgcc tcgcgccgtc gatcagcccc ggcaagacct gggaaggggt
    13921 ggcgggggcg gtcgtcgcct gcctcatcta cgctatcatt tgcgcgctgc cgggcgcgcc
    13981 attcggtccc tttgtacagg gggcgacttg gataccctat ctggccggtg ccgccctact
    14041 gtgcggcgtg agtatcgtgg gcgacttgtt cgagtccggg ctgaagcggc gtgccggggc
    14101 caaggacagc ggcggcttgc ttcctgggca cggtggcgtg ctcgaccgca tcgatagcat
    14161 catgcctacc ctgccgattg ccgccatgct tattgacctc atgacccgga catgacgccg
    14221 gaacacggca gagcggacgc gcagcggccg gggagcctgg tgatcctggg cgccaccggc
    14281 tcgatcggct cgagcacgct ggacgtggtc gcgcgccacc cacggcgcta tcgcgtgcac
    14341 gcgctgaccg cgcacgcctc cgcacaggga ctccttgcgc tgtgccagcg ccaccggccg
    14401 cgctacgccg tgctttcggg ctgcgcggaa gacgccgcac tgcgccggag ctttgccgac
    14461 gtcggctgcg agctgcggtt cggcccggcg gccctggccg atctcgctgc cgcacccgag
    14521 ggcgacacgg tcatggcggc gattgtcggt gccgccggct tgcccgcgac gctggcggca
    14581 gcacgtgccg gcaaacgcgt gctcctcgcc aacaaggaag cgctggtcat ggccgggccg
    14641 ttgttgacgc gcgcagcgcg cgacgcgggc gcggtgctgc tgccgatcga cagcgagcac
    14701 aacgcggtgt ttcagtgcct gccgccggag ggcgccggga tgcgcggcgt gcgccgcatc
    14761 cagctcaccg cctcgggcgg gccgttccgc gccacgccgg ccgagcgcct ggccggcgtc
    14821 accccgga
//
LOCUS       DBNHKE010000090         4529 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_650088, whole genome shotgun sequence.
ACCESSION   DBNHKE010000090 DBNHKE010000000
VERSION     DBNHKE010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_650088"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1185
                     /locus_tag="AC16O9_05650"
     CDS             1..1185
                     /locus_tag="AC16O9_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516135.1"
                     /translation="MTFRRLLAVAATLLCATAQAQPVKIGELNSYKTFPAFLEPYKKG
                     WQLALEEVNAAGGVNGRPLAVVSREDGGTPGEAVRVAEELITRERVALLMGTFASHVG
                     LAVSNLAARRNVVFVAAEPLTDKIVWESGNRYTFRLRPSTYMQVAMLVPEAARLGKRR
                     WAIVYPNYEYGQSATASFKQLLAAAQPGVEFVAEQAPPLGKIDAGAVVQALADARPDA
                     IFSSLFATDLQKFVREGNLRGLFRDTPVFNLLAGEPEYLDTLKDETPEGWWVTGYPWR
                     QIDTPEHRAFLETYRARWQDYPRLGSVVGYAALHTAAGALRAAASTDSEKLVAALKGL
                     RVRTPFGPIQYRAIDHQSTMGAYVGRLARRDGEGVMVDWRYADGAEYLPADAVVRALR
                     KE"
     gene            1182..3005
                     /locus_tag="AC16O9_05655"
     CDS             1182..3005
                     /locus_tag="AC16O9_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516136.1"
                     /translation="MSGSTFLLQFLNGLASASALFLVAAGLSLIFGVTRIVNFAHGSL
                     FMLGMYVALALVPHVGFWWSVPLAALAVAALGAALEALVLRRLYAAPELMQLLATFAL
                     VLLIRDFALWAWGPEDMLGPRAPGLDGAVTIAGERFPEYDLFLMLCGPLVLGVLWVLL
                     RRTRWGVLVRAATQDREMTSALGVNPRRLFASVFALGALLAGLGGAVQIPREPATLSI
                     DLQIIADAFVVVVVGGLGSIPGAFLAALLIGEIKAFSIALGYSEFTLALEFVVMAIVL
                     VLRPWGLLGRAPAAARGAAALVPPLPVPARGVWLALVAMLLGLAAVPLVSGGYALVLL
                     TDILVFALFAVSLHFITGPAGLLSFGHAAYFGLGAYGAGLLLLKAGAPMAAALVAAPF
                     VAALAALAFGWFCVRLSGVYLAMLTLAFAQITWSVVYQWDAFTGGSNGLIGVWPAPWL
                     SGKAAYYYLTLATSATAVAFLWRLLYAPFGYALRAGRDSPLRAESVGIDVRAMQWAAF
                     VFAGLFAGLAGALYAFSKGSVSPEALAVPRSVDGLVMVLLGGLHTLTGPVWGAALFTW
                     LQDAASRGFDYWRAVIGGIVLVLVLVLPGGLGGLLARRAAR"
     gene            3002..3748
                     /locus_tag="AC16O9_05660"
     CDS             3002..3748
                     /locus_tag="AC16O9_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516137.1"
                     /translation="MSALAVHSLAKSYGGVQALRGVSFSVAAGELVALIGPNGAGKTT
                     CFDVLNGLTPADAGSVELGGRSLLGLPPRRVWRLGVGRTFQLTATFGSMSVRENVQTA
                     LLSHRRRLWSLFRVAARACADEADVLLERVGILQHAERPCAVLAYGDLKRVELAMALA
                     NRPRLLLMDEPTAGMATQERGALMRLAAALAHDTGTAVLFTEHDMDVVFTHADRVLVL
                     AEGELIAAGSPAAVRADPRVREVYLGSGDA"
     gene            3741..4424
                     /locus_tag="AC16O9_05665"
     CDS             3741..4424
                     /locus_tag="AC16O9_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516138.1"
                     /translation="MLEVRALGAAYGKAQVLFGLDFTARAGEVLVLLGRNGAGKSTAL
                     KRIMGLLPPRAGEIRFDGVRIDAWPPHRIARLGLGYVPEERRIFTDLTVRENLEVGRQ
                     APRSAAAYWTPEKLFELFPNLGRMQDRLGGRMSGGEQQMLAVARTLMGNPRAILLDEP
                     AEGLAPIVVGQVAAAVRELKAQGLCVVLSEQNVRFARGVADRACLLEQGRAVASGPLE
                     DVQARLALG"
BASE COUNT          540 a         1545 c         1652 g          792 t
ORIGIN      
        1 atgaccttcc gacgcctgct tgcggtagcc gccacgctgc tctgcgccac ggcgcaggcg
       61 cagcccgtca agatcggcga gctgaacagc tataagacat tcccggcatt cctcgagccg
      121 tacaagaagg gctggcagct cgccctggag gaagtgaacg ccgcgggtgg cgtcaacggc
      181 cggccgctcg ccgtggtgtc gcgcgaggac ggcggcacgc cgggcgaggc ggtgcgcgtg
      241 gccgaggagc tgatcacgcg ggaacgggtc gcgctgctga tggggacgtt cgcctcccac
      301 gtcggcctcg cggtgtccaa tctggcggcg cgacgcaacg tggtgttcgt cgccgcggag
      361 ccgctcaccg acaagatcgt ctgggagagc ggcaatcgct atacgttccg cttgcggccc
      421 tcgacctaca tgcaggtggc gatgctggtg ccggaggccg cgcgcctggg caaacgccgc
      481 tgggcgatcg tctatcccaa ctatgaatac ggccagtcgg ccaccgcgtc cttcaagcag
      541 ctgctcgcag cggcacagcc cggcgtggaa ttcgttgcgg agcaggcgcc gccgcttggc
      601 aagatcgacg ccggtgcggt ggtgcaggca ctggccgatg cgcggccgga cgcgatcttc
      661 agctctctgt tcgccaccga cctgcagaag ttcgtgcgcg aaggcaatct gcgcggcctg
      721 tttcgcgaca caccggtgtt caacctgctc gccggcgagc ccgagtatct cgacacgctc
      781 aaggacgaga cccccgaggg ctggtgggtc accggctatc catggcgcca gatcgacacg
      841 cccgagcacc gcgcattcct cgagacctac cgcgcgcgct ggcaggacta cccgcgcctg
      901 ggctcggtgg ttggctacgc ggcgctgcac acggcggcag gcgcattgcg cgccgctgcg
      961 tccaccgaca gcgagaagct ggtcgcagcg ctgaagggct tgcgcgtgcg cacgcccttc
     1021 gggccgatcc agtaccgcgc gatcgaccac cagtccacca tgggcgccta cgtcggccgg
     1081 ctggcgcggc gtgatggcga aggcgtgatg gtcgactggc gctatgccga cggcgccgaa
     1141 tacctccccg cggacgcggt ggtgcgggcg ctgcgcaagg agtgagcggc tccacgttcc
     1201 tgctgcagtt cctgaacggc ctggcgagcg cctcggcgct atttctggtc gcggccggcc
     1261 tgtcgctgat cttcggcgtc acgcgcatcg tcaatttcgc gcacggctcg ttgttcatgc
     1321 tgggcatgta cgtcgcactc gcgctggtgc ctcatgtcgg cttctggtgg agcgtgccgc
     1381 tcgctgcgct cgccgtggcg gcgctcggcg cggccctcga ggccctcgtg ctgcgccgct
     1441 tgtacgccgc gccggaactg atgcagctgc ttgccacgtt cgcgctggtg ctgctgatcc
     1501 gcgacttcgc gctttgggcc tgggggccgg aggacatgct cgggccgcgg gcaccgggac
     1561 tggatggcgc cgtgacaatc gccggcgagc gctttccaga gtacgacctg tttctcatgc
     1621 tctgcggtcc gctggtgctg ggcgtgctgt gggtcctcct gcggcgcacg cgctggggcg
     1681 tgctggtgcg cgccgcgacc caggaccgcg agatgaccag tgcgctcggc gtgaacccgc
     1741 ggcgcctgtt cgccagcgtg ttcgcgctcg gggccctgct ggcaggcttg ggcggcgcgg
     1801 tgcagatccc acgcgagccg gcgacgcttt ccatcgacct gcagatcatc gccgacgcct
     1861 tcgtcgtggt cgtggtcggc ggactgggtt cgattcccgg ggcgttcctc gccgccctgc
     1921 tgattggcga gatcaaggcg ttcagcatcg cgctgggcta ttccgaattc accctggcgc
     1981 tggagttcgt ggtcatggcg atcgtgctgg tgctgcggcc ctggggactg ctcggacgcg
     2041 cacccgcggc ggcgcgcggc gccgctgcgc tggtgccacc gctcccggtt cctgcccgcg
     2101 gcgtgtggct tgccctggtg gcgatgctgc tcggactcgc cgcggtgccg ctcgtctcgg
     2161 gtggctacgc cctcgtgctg ctgaccgaca tcctcgtctt cgcgctgttc gcggtgagcc
     2221 tgcacttcat caccggcccc gcggggctgc tgtccttcgg ccatgccgcc tacttcgggc
     2281 tcggcgccta cggtgctggc ttgctgctgc tgaaagcagg cgcaccgatg gccgccgcac
     2341 tcgtcgcggc gccgttcgtc gccgcgcttg ccgcgctcgc cttcggctgg ttctgcgttc
     2401 gcctgtcggg cgtgtacctg gcgatgctca cgctcgcgtt tgcgcagatc acctggtcgg
     2461 tggtctacca gtgggatgca ttcaccggcg gctccaatgg cctgatcggc gtctggcccg
     2521 cgccgtggct ctcgggcaag gctgcgtact actacctgac gctggccacc agcgccacgg
     2581 cggtcgcgtt tctgtggcgc ctgctgtacg cgccgttcgg ttacgcgctg cgtgcggggc
     2641 gcgattcgcc gctgcgcgcg gaatccgtcg gcatcgacgt acgggcgatg cagtgggcgg
     2701 ctttcgtctt cgcggggctg ttcgccggcc tggccggcgc gctgtacgcc ttttccaaag
     2761 gctcggtctc cccagaggcg ctggccgtgc cgcgctcagt ggacggcctg gtcatggtgc
     2821 tgctcggcgg cttgcacacg ctgactggcc cggtgtgggg cgcagcgctg ttcacgtggc
     2881 tgcaggacgc cgcgtctcgc ggcttcgact actggcgcgc ggtgatcggc ggcatcgtgc
     2941 tggtcctagt gctggtgctg ccgggcgggc taggcgggtt gcttgctcgg agggccgcac
     3001 gatgagcgcg ctcgcggtgc atagcctggc caagtcctac ggcggcgtgc aggcgctgcg
     3061 cggcgtgagc tttagcgtcg ccgcaggcga gctggtggcg ctgatcggtc cgaacggcgc
     3121 cggcaagacg acctgctttg acgtgctcaa cgggctgacg cctgccgacg ccggctcggt
     3181 cgagcttggc ggccgcagcc tcctcggcct gccaccgcgg cgcgtctggc gcctgggcgt
     3241 cgggcgcacc ttccagctca ccgccacctt tggctcgatg agcgtgcgcg agaatgtgca
     3301 gaccgccctg ctctcgcacc ggcgccgcct gtggtcgctc ttcagggtcg cggcgcgcgc
     3361 ctgcgccgac gaagccgatg tgctgctcga gcgcgtcgga atcctgcagc acgcggagcg
     3421 accgtgcgcc gtgctcgcct acggtgacct gaagcgggtc gagctcgcca tggcgctcgc
     3481 caaccggccg cgcctgctgc tgatggacga gccgacggcg gggatggcaa cccaggagcg
     3541 cggcgcgctg atgcggcttg ccgcagcgct ggcgcacgat acgggcaccg cggtgctgtt
     3601 caccgagcat gacatggacg tcgtctttac ccacgctgac cgtgtcctgg tactcgccga
     3661 gggtgaactg attgccgcgg gcagcccggc agcggtgcgc gccgatccgc gcgtgcgcga
     3721 ggtctaccta gggagcggcg atgcttgagg tgcgcgccct gggcgccgcc tatggcaagg
     3781 cgcaagtcct gttcggactg gatttcaccg cgcgggccgg cgaggtgctg gtgctgctcg
     3841 gacgcaatgg cgccggcaag tccaccgcgc tgaagcggat catggggctg ctgccgcccc
     3901 gggcgggcga gatccgcttc gacggcgtgc gcatcgacgc gtggccgccc catcggatcg
     3961 cgcggctcgg gctcggctat gtgcccgagg agcggcgcat cttcaccgat ctcacggtgc
     4021 gcgagaatct cgaggtgggc cgccaggcgc cccggtcggc ggccgcgtat tggacgcccg
     4081 agaagctgtt cgagctgttc ccgaacctcg ggcgcatgca ggaccgcctc ggcggccgga
     4141 tgtcgggcgg cgagcagcag atgcttgccg tcgcgcgcac gctgatgggc aatccgcgcg
     4201 ccatccttct cgatgagccc gccgaaggcc tggcgcccat cgttgtcggc caggtggcgg
     4261 cggcggtccg cgagctcaag gcgcagggac tgtgcgtggt gctgagcgag cagaacgtgc
     4321 gcttcgcgcg cggggtcgcc gatcgggcgt gcctgctcga gcagggccgc gcggtcgcct
     4381 cgggtccgct cgaggacgtg caggcgcggc ttgcactcgg ctgaagcgct caggccacga
     4441 cggcaggcgg cgcagcgccc gcggtgacga cccggttgcg gccggcgtcc tttgcttcgt
     4501 acagggcggt atcggcggcg gtcagcagc
//
LOCUS       DBNHKE010000091        14853 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_437989, whole genome shotgun sequence.
ACCESSION   DBNHKE010000091 DBNHKE010000000
VERSION     DBNHKE010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_437989"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="AC16O9_05670"
     CDS             complement(<1..1571)
                     /locus_tag="AC16O9_05670"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6516139.1"
                     /translation="MKVLVRVAFAALVLIVVAAAGGYFHLRQSLPQEEGEVRLAGLAK
                     PVEVLRDSYGVPHISAQSLADAIHALGYVHAQDRLWQMEVNRRTAAGRLAEAFGEGAL
                     ETDRFLRTLGIRRAAEANLKHLDAETQSLLEAYAAGVNAFLASKPVLPLEFWMTGVNP
                     EPWTPADSLGWVKMMAWDLGGNWRNELLRLRLSKTLSNDRLHELLPPYPGETYPALPD
                     LRQLYGGLERDSVRLANVNGDRPHFPQRPIAKMRSVPIYEALEGLGSNNWVVSGAKSA
                     TGKPLLANDPHLGLTAPTVWYFAHLTAPGIDVIGATLPGVPAVVLGHNTHFAWGATNT
                     GPDVQDLYLERLDGAGNYLTPEGPRAFTLIDEVIKVKGAADVRLQVRVTRHGPVISDV
                     SRAALAAVPRGHVIAFQWTALRDDDLTVQSALKLVRARDWPSFLAAMRDYHSPQQNYV
                     YADTAGNIGFIAAGRVPVRKPANDLHGMAPAPGWQAKYDWAGFIPFAELPHAYNPPSG
                     QVVTANHRITPPGYA"
     gene            1668..2864
                     /locus_tag="AC16O9_05675"
     CDS             1668..2864
                     /locus_tag="AC16O9_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR6516140.1"
                     /translation="MLYSLYEAQHAWLTPFRLIAEAQRGWFAHPFMPWADQPLAKQIA
                     ASNDLFLRVTQRYEKPEWRIAGADIELALDKPFCKLLRFTPTTPRTRAAPKVLVVAPL
                     SGHHATLLRDTVRTLLAEHDVWVTDWVDARLVPLAAGPFHLADYVEYVQEFIRLLSPD
                     AHVISVCQPTVPVLCAIALMAEAREPHAPKTMTMMGGPIDARKSPTAVNNLALTRPLS
                     WFERHVIHRVPAKYPGYHRRVYPGFLQHLGFVAMNPDRHLNAHTDYFNHLLEGDDESA
                     ERHREFYDEYNAVIDLPAEYYLDTVKAVFQDFALPKGRMVVRDRLVRPQTIRDTALLT
                     IEGELDDISGNGQTEAAHLLCLNIDKDRREHYLAPRVGHYGIFSGRRWREMIYPRVRE
                     FIRKHG"
     gene            complement(2882..3508)
                     /locus_tag="AC16O9_05680"
     CDS             complement(2882..3508)
                     /locus_tag="AC16O9_05680"
                     /inference="COORDINATES: protein motif:HMM:NF020905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HIR6516141.1"
                     /translation="MLSAAEQVPGVVISSEVILARAGWSRELSRGQVLRIIDLEGRQA
                     VDFLCYNARDYEDRYAAADTMKINGSIFVRKGTVIYSVNCNPLLTVTEDTCGFHDTIG
                     GCCSSALNRRRYGKPDDPNCRQNFLDQLGRYGMGPRDMVANLNFFMYVPVAADGSMDM
                     GPSISKPGDYVDLRAEMDVLAVISNCPQMNNPVNDYNPTPVRAIIYQP"
     gene            complement(3522..3968)
                     /locus_tag="AC16O9_05685"
     CDS             complement(3522..3968)
                     /locus_tag="AC16O9_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516142.1"
                     /translation="MNLLIVDDHPVVAEYLRNAAGRAVPDAVIRIAGDLETAFESLQE
                     NPAELVLLDLGLPGCGGVEAVLRFRQAFPDTRVVVISSEDERNAIRGCIALGAAGFIP
                     KTAGPREMVKALRAVAEGGRYIPAPERGPEASVRLEGRRGTGTAGY"
     gene            complement(4140..4784)
                     /locus_tag="AC16O9_05690"
     CDS             complement(4140..4784)
                     /locus_tag="AC16O9_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6516143.1"
                     /translation="MNVLIVDDHPMVAEYLRGAAGRAIPDAVVRIAGDLEAALESMRD
                     APADMVMLDLGLPGCGGIESLLRFRESFPDVRVVVISSEDGAAAIRGALAAGAAGFIP
                     KTAGPNVMVNALRLVAEGGRYIPPEALGELFADLTGASAGARERSPSGRLTERQREVL
                     KRLLAGRTVTQIAEDLGIAESTAKHHTLAVYAAFRVSSRADLILAAARRGQATG"
     gene            4992..5282
                     /locus_tag="AC16O9_05695"
     CDS             4992..5282
                     /locus_tag="AC16O9_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516144.1"
                     /translation="MPVTATSLKVPPALKARIEKLSKRTGESAHAFMLRALEGRVEAL
                     ERYQEFLDDGIRADEAMLRSGLGYAASEVHEYLQARIQDRATRRPKPVRWQK"
     gene            5270..5563
                     /locus_tag="AC16O9_05700"
     CDS             5270..5563
                     /locus_tag="AC16O9_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR6516145.1"
                     /translation="MAEVIYSAEALADLERIIDFLLETSPETAEKALAQIRSAITILA
                     SHPHIGRRVDRVRRELVITYGASGYLALYRHDARPDVTRILRVRHQREAGYID"
     gene            complement(5583..5963)
                     /locus_tag="AC16O9_05705"
     CDS             complement(5583..5963)
                     /locus_tag="AC16O9_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIR6516146.1"
                     /translation="MEAVLAIHEEVLAAHGGLGGLRDRALLESAVAAPQASFGGKPLI
                     RDPIEVAAAYLFYLCRNHPFVDGNKRTALAAALVFLDANGLLPDPGAPARKIDDWESL
                     VLDVASAKLDRDQASRKLQALLKR"
     gene            complement(5986..6213)
                     /locus_tag="AC16O9_05710"
     CDS             complement(5986..6213)
                     /locus_tag="AC16O9_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR6516147.1"
                     /translation="MLKTITKIGNSQGLIFDAALRELSGLQPGDQVNVTVHEGGAIVI
                     TPLRAAVPAQEARKSARALIRRNSRLFKRLA"
     gene            complement(6255..7166)
                     /locus_tag="AC16O9_05715"
     CDS             complement(6255..7166)
                     /locus_tag="AC16O9_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6516148.1"
                     /translation="MGSDFAKGLVWLAIAIGSWGALFSVAKRTLPVLDAFFLGSFRYA
                     FGVLLFVAILWAVEGRQALRYGGRGVPAALFGLIGFCGFNLLVWWGLVHTRPEHAAII
                     MALQSPMTAIAVWLTQGRRPHPFTIGCVAAAIAGVVLVVTKGSLEDVFAGGSLLGDLL
                     VFLGALSWVVYTMAGWHFSGWSPLRMTVLTAIPGAIGLVAINAAAIGLGYVELPTFEA
                     VWSVKWQLAYFVVFTVVLGVLGFNNGVKYLGALNTMLMLNLIPVIVFAIEAWLGRSYA
                     AIELAGAAVVIGALVANNLYLRQQRAR"
     gene            complement(7245..8912)
                     /locus_tag="AC16O9_05720"
     CDS             complement(7245..8912)
                     /locus_tag="AC16O9_05720"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR6516149.1"
                     /translation="MERIWLKSYPPGMPADVTWNEFKSVGELFETSCRKFADQPAFHC
                     MGKTLSFGEIDKLSRDFAAWLQSKGLAKGARVAIMMPNCLQYPIVLFGTLRAGYTVVN
                     VNPLYTARELEHQLKDSGAEAIVILENFAHTLEEVLPRSPVKHIAVAALGDLLGVKGH
                     IVNFVVRNVKKMVPAFELPGAERFNAVLKQGAGLAMKPFVVGQGDLAFLQYTGGTTGV
                     SKGAMLTHENILANIEQSSWNLTAALGGTTPVIITALPLYHIYSLTVNCLLMAKMGGF
                     NILITNPRDIPAFVKELGKHRYNMITGVNTLFNAFLNNPEFHKLDFSHVKVCGAGGMA
                     LQKAVAERWKQVTGKVILEGYGLTETSPVAAMNPPNLTQFNGSIGLPVPSTDFEIRDD
                     DNKPVPLGQPGEICIKGPQVMKGYWQRPDETAKVLDKDGWLHTGDVGVMDPQGFVRIV
                     DRKKDMILVSGFNVYPNEIEQVVAMHPGVLEVAAIGVPDEHSGEVPKLFIVKRDPALT
                     EQQVLEFCKDKLTGYKRPKHVEFRTELPKTNVGKILRRALRDEKKAA"
     gene            complement(8941..10137)
                     /locus_tag="AC16O9_05725"
     CDS             complement(8941..10137)
                     /locus_tag="AC16O9_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6516150.1"
                     /translation="MRSVPIILIAAHIFSMLGFSTYPALLPGLQGEWQISNSEAGIIA
                     SLFFAGYIATVSAWTALTDRVDGRYVYAAGSALAFAGCAGFGLLAQGFLSACAFQALL
                     GAGIAGTYMPGLRLLSDVVHGPAQSRYIAFYTSFFGIGAAISFALAGFIAPLWGWRAA
                     FVLCAAGSVVSGVMLFASLPAKEPAGSTHSILFPLAAWRKVLANRAAAGYMIGYGVHC
                     LELFGSRGWMVAFLSFAAGLGVAGGAPWGPAAIAAVANAAAVPASILGNEMALRIGRR
                     RWILIVMTASSLTGIGLAFSAPLHWAFVVALLMVYSMLIMAESATLTAGYVAAAPPEL
                     RGAAMGLYSLVGFGGGMLGPAVFGAALDIAGGQASLLAWACGYAAIGAGCLAAPLVVR
                     VLRAAR"
     gene            complement(10196..10972)
                     /locus_tag="AC16O9_05730"
     CDS             complement(10196..10972)
                     /locus_tag="AC16O9_05730"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6516151.1"
                     /translation="MSTEFIVVETRGRVGVVTLNRPKQMNALNAQLMQELAQALYAFD
                     ADDGVGCIVITGNDKAFAAGADIAAMKDFGYMDAFRSNYITRDWEHIRNIRKPVLAAV
                     AGFALGGGCELAMMCDLAIAADTAKFGQPEINLGVMPGSGGTQRLPRFTSKALAMDMI
                     LTARMIGADEALRAGLVSRVVPAAKLMEEAMAMAEKIAGYSLPAVMMAKESVNRAYEG
                     GLAEGVLFERRLFHSMFALEDQKEGMAAFVEKRKPSFKNK"
     gene            complement(10969..11580)
                     /locus_tag="AC16O9_05735"
     CDS             complement(10969..11580)
                     /locus_tag="AC16O9_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HIR6516152.1"
                     /translation="MPQVYSIDGLVPVVDPSAFVHESAVLVGDVIVGARAYVGPGACL
                     RGDFGRIVVEEGANIQDTCMLHGFPGKDTVVGREATIGHGAVLHGCVIRRGALVGMNA
                     VVNDNAEVGEEAMVAALAFVKAEAKIPARGLAAGIPARVLRELTAEELAWKTDNMHLY
                     QKLTERSAKTMRRVEALTAVEPGRKRIDIPGAIPLSEMKARSK"
     gene            11618..12397
                     /gene="paaG"
                     /locus_tag="AC16O9_05740"
     CDS             11618..12397
                     /gene="paaG"
                     /locus_tag="AC16O9_05740"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HIR6516153.1"
                     /translation="MPPVLVSTADGVMTITLNRPEKLNAFVRDMHGLLREAMAQAEGD
                     AAIRALLLTGAGRGFCAGQDLAERDMNDPNLDLGGGLEASYNPLIRRLRALPKPVVCA
                     VNGIAAGAGANIALACDLVLAARSAAFIQAFSKIALVPDCGGSYWLPRLVGLQRAMGM
                     ALLAERVSAEDAERWGLVWKCVDDAKLMDEATSLARTLAAGPTHTYGMIKKALYASSG
                     NSLDQQLDLERDFQREVGRHGDYREGVAAFLEKRQARFTGR"
     gene            12397..13686
                     /gene="paaK"
                     /locus_tag="AC16O9_05745"
     CDS             12397..13686
                     /gene="paaK"
                     /locus_tag="AC16O9_05745"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HIR6516154.1"
                     /translation="MAKAKKFPLEPIEKGSRKSLRELQLRRLKWSLKHAYDNVPAYRR
                     KFDAAGVKPKDLKSLADLSKFPFTAKADLRENYPFGMFAVPMDRIVRIHASSGTTGKP
                     TVVGYTQKDIDTWSHVMARSFRAAGARPGDKVHNAYGYGLFTGGLGAHYGAEKLGLAV
                     IPLSGGMTERQVQLIQDFKPDIITVTPSYMLAIADEMEKQGIDPKKVSLRIGMFGAEP
                     WTHEMRKQIEARTSLDAIDIYGLSEVIGPGVAQECIETKDGLTVWEDHFYPEIVDPQS
                     GAVLPEGEQGELVFTSLTKEAMPVIRYRTRDLTRLLPPTARSMRRMEKITGRSDDMLI
                     IRGVNVFPTQIEELILKQPELAPHYVLELSKDGPMDHLTVLVELAPGAADADSAAKLK
                     HHVKSYIGVTADVKIVPISTIERSIGKAKRVVDKRAK"
     gene            13683..14657
                     /locus_tag="AC16O9_05750"
     CDS             13683..14657
                     /locus_tag="AC16O9_05750"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HIR6516155.1"
                     /translation="MILGLTRKELEDGARLVHEAVPPTPQYSWPLLNERAGTEVWVKH
                     ENHTATGAFKVRGGLVLAQEIKRRDPKVAGVISATRGNHGQSVAFAGRRHGMRSVIVV
                     PHGNSREKNAAMRAWGAELIEHGRDYDEAREHAAKLAKAEGLFYIGPFHRELVVGVGS
                     YAMELFLGAPQLDEVYVPIGCGSGICGVIAWRDALGLKTRVVGVVSENAACYAHSFES
                     GRPVETKTANTFADGVAVRVPVAEALEAILAGADRIVQVSDDEVKAAMRDFYRCTHNL
                     AEGAGAAPLAALMKDGARKGSRFAVILSGGNIDREMYLEALGGNATSG"
     gene            14690..>14853
                     /locus_tag="AC16O9_05755"
     CDS             14690..>14853
                     /locus_tag="AC16O9_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012392.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6516156.1"
                     /translation="MENVIARARSYLDEHRGERVTLSDLARAVGASPFHLQRRFKQAF
                     GLSPKDYQREA"
BASE COUNT         2516 a         5098 c         4930 g         2309 t
ORIGIN      
        1 tgcgcgtagc ccggcggcgt gatgcgatgg ttggcggtca ccacctgccc gctcggcggg
       61 ttgtaggcgt gcggcagctc ggcgaagggt atgaagcccg cccagtcgta tttcgcctgc
      121 cagcccggcg ccggcgccat gccgtgcagg tcgttggcgg gctttcgcac cggcacgcgc
      181 ccggcggcga tgaagccgat gttgccggcg gtgtcggcgt agacgtagtt ctgctgcggc
      241 gagtggtagt cgcgcatcgc ggcgagaaag ctcggccagt cgcgcgcgcg caccagcttc
      301 agcgccgact gcacggtgag gtcgtcgtcg cgcagcgccg tccactggaa cgcaattacg
      361 tgcccgcgcg gcaccgccgc gagcgcggcg cgcgacacgt ccgagatcac cgggccgtgg
      421 cgcgtgacgc gcacctgcag gcgcacgtcg gccgcgccct tcaccttgat cacctcgtcg
      481 atcaacgtga aggcgcgcgg gccctctggc gtgaggtagt tgcccgcgcc gtcgaggcgc
      541 tcgagataga ggtcctgcac gtcgggcccg gtgttcgtcg cgccccacgc gaagtgcgtg
      601 ttgtgcccga gcacgaccgc gggcacgccc ggcagcgtgg cgccgatcac gtcgatgccc
      661 ggcgccgtaa ggtgcgcgaa gtaccacacc gtgggtgcgg tgagcccgag gtgcggatcg
      721 ttggcgagga gcggcttgcc cgtggcgctc ttcgcgcccg acaccaccca gttgttcgag
      781 cccagcccct cgagggcttc ataaatgggg acagacctca ttttcgctat aggacgttgg
      841 ggaaaatgag gtctgtcccc atttacgttt gccagccgca cgctgtcgcg ttccaggccg
      901 ccgtagagct ggcgcaggtc cgggagcgcg gggtaggtct cgccgggata cggcggcagc
      961 agctcgtgca ggcgatcgtt ggagagcgtc ttcgaaagcc gcaggcgcag cagctcgttt
     1021 cgccagttgc cgcccaggtc ccaggccatc atcttcaccc agccgagcga atcggccggc
     1081 gtccacggct cggggttgac gccggtcatc cagaattcca gcggcagaac cggcttcgac
     1141 gcgagaaacg cgttcacgcc ggcggcgtag gcctcgagca ggctttgcgt ttcggcgtcg
     1201 aggtgcttca agttggcttc ggccgcgcgc cggatgccga gcgtgcgcag gaaccggtcg
     1261 gtctcgagcg cgccctcgcc gaaggcttcg gcgagccggc ccgcggccgt gcgccggttg
     1321 acctccatct gccagagccg gtcctgcgca tgcacgtagc cgagcgcatg aatcgcatcg
     1381 gcaaggctct gcgcgctgat gtgcggcacg ccgtagctgt cgcgcagcac ttcgaccggc
     1441 ttggcgaggc ccgcgaggcg cacctcgcct tcttcctggg gcagggactg gcgcaggtgg
     1501 aagtagccgc cagccgccgc gacgacgatg agaactagtg ccgcaaaggc gacccggacc
     1561 aggactttca tcgtctaagc atacgatttc tcggtgaatt cgaccaatgc ggcagcgccg
     1621 caaatctgct ataaacgtgc cgtcaccatg gcattgggag ctcgtagatg ctctacagcc
     1681 tgtacgaagc gcagcatgcc tggctcacgc ctttccgcct gatcgcggaa gcgcagcggg
     1741 gatggttcgc ccatccgttc atgccctggg cggaccagcc gctcgcgaag cagatcgccg
     1801 cctcgaacga cctgttcctg cgcgtcacgc agcgctatga gaagcccgag tggcgcatcg
     1861 ccggcgccga catcgagctc gcgctggaca agcccttctg caagctgctg cgcttcacgc
     1921 cgaccacgcc gcgcacgcgc gccgcgccca aggtcctcgt cgtcgcgccg ctctccgggc
     1981 accacgcgac gctgctgcgc gacaccgtgc gcacgctcct ggcagagcac gacgtgtggg
     2041 tcaccgactg ggtcgacgcg cgcctcgtgc cgctcgccgc ggggccgttc cacctcgccg
     2101 actatgtcga gtacgtgcag gaattcatcc gcctgctgtc gcccgacgcg cacgtcatct
     2161 cggtgtgcca gcccacggtg ccggtgctgt gcgccatcgc gctgatggcc gaagcgcgcg
     2221 agccgcacgc gccgaagacc atgacgatga tgggcgggcc gatcgacgcg cgcaagagcc
     2281 ccacggcggt caacaacctc gccctgacgc ggccgctctc ctggttcgag cgccacgtca
     2341 tccaccgcgt gccggcgaag tacccgggct accaccggcg cgtgtatccg ggcttcctgc
     2401 agcacctggg cttcgtcgcc atgaacccgg accggcacct gaacgcgcat accgactact
     2461 tcaaccatct gctggaaggc gacgacgaat cggccgagcg ccaccgcgag ttctacgacg
     2521 agtacaacgc ggtgatcgac ctccccgccg agtactacct cgacaccgtg aaggcggtgt
     2581 tccaggactt cgcgctgccc aagggccgca tggtcgtgcg cgatcgcctg gtgcggccgc
     2641 agaccatccg cgacaccgcg ctcctcacca tcgagggcga gctcgacgac atctcgggca
     2701 acggccagac cgaggccgcg cacctgctgt gcctcaacat cgacaaggac cgccgcgagc
     2761 actacctcgc gccgcgcgtg gggcactacg gcatcttcag cgggcggcgc tggcgcgaga
     2821 tgatctaccc gcgcgtgagg gaattcatcc gcaagcacgg ataaatgggg tcagacccca
     2881 tttacggctg atagatgatc gcccgcaccg gtgtcgggtt gtagtcgttc accgggttgt
     2941 tcatctgcgg gcagttggag atgacggcaa gcacgtccat ctccgcgcgc aggtcgacat
     3001 agtcgccggg cttcgagatg ctcggcccca tgtccatcga gccgtcggca gcgaccggca
     3061 catacatgaa gaagttgagg ttcgcgacca tgtcgcgcgg ccccatgccg tagcggccga
     3121 gctggtcgag gaagttctgc cggcagttgg gatcgtccgg cttgccgtag cgccggcggt
     3181 tgagcgccga cgagcagcag ccgccgatcg tgtcgtggaa gccgcaggtg tcctcggtca
     3241 cggtcaacag cggattgcag ttcaccgagt agatcaccgt gcccttgcgc acgaagatgc
     3301 tgccgttgat cttcatcgtg tcggcggcgg cgtagcggtc ctcgtagtcg cgcgcgttgt
     3361 agcacaggaa atccactgcc tggcggcctt cgagatcgat gatgcgcagc acctggccgc
     3421 gcgagagctc gcggctccag ccggcgcgcg ccaggatgac ttcggatgag atgacgactc
     3481 cggggacctg ctcggccgcg ctcagcatgg cgcggacttt atcagtatcc ggccgtcccg
     3541 gtcccccgtc tgccctccag acgcacggac gcctcgggcc cacgctcggg cgccgggatg
     3601 tagcgcccgc cttcggcgac agcgcgcagc gctttcacca tctcccgcgg cccggcggtc
     3661 ttgggaatga agcccgccgc cccgagcgcg atgcagccgc ggatcgcgtt gcgctcgtct
     3721 tcgctggaga tcaccaccac ccgcgtgtcc ggaaacgcct ggcgaaagcg cagcaccgcc
     3781 tcgacgccgc cgcagcccgg cagtccgagg tcgagcagca ccagctccgc gggattctcc
     3841 tgcaacgatt cgaaggccgt ctcgaggtcg cccgcgatac ggatgactgc atccggcacc
     3901 gcgcgcccgg cggcgttacg caggtactcc gccaccaccg gatggtcgtc gacgatgagg
     3961 aggttcatgg cgggccttgc ctgagggagg gacgaagatt gttcgccttg ggccggcacc
     4021 tcacaagtgc ggcaggcgag cgggtgtggc ttctgtcacg ttatggcgcg aaaagtcgct
     4081 ggcgtgcggc ctgagttcgc gagaaccgat cgttcgccgc aagggcgaga agagactgct
     4141 caccccgtcg cctgccccct ccttgccgcg gccaatatca ggtccgcccg cgacgacacg
     4201 cggaacgccg catagaccgc gagcgtatgg tgcttggccg tggattcggc gatgcccagg
     4261 tcttccgcga tctgggtgac cgtcctcccg gcgagcaggc gcttcagcac ctcgcgctgg
     4321 cgctcagtga gccggccgct tggcgagcgc tcgcgcgcgc cggccgaggc gccggtcagg
     4381 tcggcgaaca gctcgcccag tgcttccggc ggaatgtagc gcccgccttc ggcaacgagc
     4441 cgcagcgcgt tcaccatgac attcgggccc gcggtcttgg ggatgaagcc cgccgcgccc
     4501 gcggccagcg cgccgcgaat cgcggcggcc ccatcctcgc tggagatcac caccacgcgc
     4561 acgtcgggaa aggactcgcg aaagcgcagg agcgactcga tgccgccgca tcccggaagc
     4621 cccaggtcga gcatcaccat gtcggccggg gcatcgcgca tcgattcgag cgccgcctcg
     4681 agatcgcccg cgatgcgcac caccgcgtcc ggaatcgccc ggccggccgc gccgcgcagg
     4741 tactccgcga ccatcgggtg gtcatcgacg atgaggacgt tcatggtggc ctggacggta
     4801 tcgagttcga attgttcgat accgggtaac gcacgacaag ggcaggcggt ggacagaaca
     4861 ttcgtggagg acggcaagcg cctgatttgc aagcggggcg ccctcagaca tctcagaaaa
     4921 agattgtttg ggacgcggtt tcggtgcctg gcgttgccgg gtcgagagtc aggtagtact
     4981 atgtgactac catgccagtc accgccacga gcctgaaagt cccgcctgct ctgaaggctc
     5041 ggattgagaa actctccaag cgcacgggcg aatccgcgca cgccttcatg ctgcgcgcgc
     5101 tcgaaggccg ggtcgaagcc ctggagcgct accaggaatt cctcgacgac gggattcgcg
     5161 ccgatgaagc gatgcttcgc agcgggctag gctatgccgc gagcgaggtg cacgagtatc
     5221 ttcaggccag gatccaggac cgcgctacac gccggccgaa gccggttcga tggcagaagt
     5281 aatctactcg gccgaggccc tcgccgatct cgagcgcatc atcgattttc ttctggagac
     5341 gtcgcctgaa acggcggaga aagcgcttgc ccagatccgc agcgcaatca cgatcctcgc
     5401 ctcgcacccc catataggcc gacgcgtgga cagggttcga cgcgagctcg tgattaccta
     5461 tggcgcgtca ggctaccttg ccctgtaccg gcacgacgcc aggccagacg tgacgcggat
     5521 cctccgggtc aggcatcagc gggaagcggg ctacatcgac taggcgatgt cccgcgcgtc
     5581 cgctatcgct tcagcaatgc ctggagcttt cgtgacgcct ggtcgcggtc gagcttggcg
     5641 ctcgccacgt cgagcaccaa ggactcccag tcatctatct tccttgcagg cgcgccagga
     5701 tcaggcagca ggccgttggc gtccagaaac accagcgccg cagccaaggc ggtacgcttg
     5761 ttcccgtcaa cgaaaggatg gttgcggcaa aggtagaaga gataggccgc cgccacctcg
     5821 atcgggtccc ggatcagcgg cttgccgccg aatgacgcct gcggagccgc gaccgcggat
     5881 tccagcagtg cgcgatcccg cagcccgccc agcccgccgt gcgccgccag cacctcctcg
     5941 tgaatggcga ggaccgcctc cacgtcaagg tgccgcagcg cgcgctcagg caaggcgctt
     6001 gaacaggcgg ctgttgcggc gaatgagcgc ccgcgccgac ttgcgggcct cttgcgccgg
     6061 aaccgccgcg cgcagcgggg taatgacgat cgccccgccc tcgtgcaccg tcacgttgac
     6121 ctggtcgccg ggctgcaggc cgctcagttc gcgcagcgcg gcatcgaaga tcagcccctg
     6181 ggagttcccg atcttggtga tcgtcttcag catcgggcga tgttaaacga tgtttaacgc
     6241 tgtgtccagc ctctctacct tgccctctgc tggcgcaagt acaggttatt cgccaccagc
     6301 gccccgatca ccaccgccgc ccccgccagc tcgatcgccg cgtagctccg ccccagccac
     6361 gcctcgatcg cgaacacgat caccgggatg aggttcagca tcagcatggt gttcagcgcg
     6421 ccgaggtact tcacgccgtt attgaacccg agcacgccga gcaccacggt gaagacgacg
     6481 aagtaagcga gctgccactt caccgaccac acggcttcga acgtcggcag ctcgacgtag
     6541 ccgagcccga tcgccgcggc gttgatcgca acgaggccga tcgcgcccgg gatcgccgtc
     6601 agtaccgtca tcctgagcgg cgaccaaccc gagaagtgcc agcccgccat cgtgtagacc
     6661 acccagctca gcgcgccgag aaagacgagc aggtcgccaa gcagcgagcc gcccgcgaag
     6721 acatcctcga ggctgccctt ggtcaccacc agcaccacgc ccgcgatcgc cgcggccacg
     6781 cagccgatgg tgaatgggtg cggccgccgg ccctgcgtga gccacacggc gatcgccgtc
     6841 atcggcgact gcagcgccat gatgatcgcg gcgtgctcgg ggcgcgtgtg gacgagcccc
     6901 caccagacga gcaggttgaa gccgcaaaaa ccgatcaggc cgaagagcgc tgccggcacg
     6961 ccgcgcccgc catagcgcag cgcctggcgc ccctccaccg cccagaggat cgcgacgaag
     7021 agcaggacgc cgaaggcgta gcgaaacgag ccgaggaaga acgcatccag caccggcagc
     7081 gtccgcttcg ccacagagaa gagcgcgccc caggagccga tggcaatggc gagccagacg
     7141 aggcccttgg cgaagtcgga acccacaaga aaaaagggga cagacctcat ttatctcatc
     7201 tgcggcgaca gatgagataa atgaggtctg tccccattta gttgttacgc ggccttcttt
     7261 tcgtccctga gcgcgcggcg caggatcttg ccgacgttgg tcttcggcag ctcggtgcgg
     7321 aactccacgt gcttgggccg cttgtagccg gtgagcttgt ccttgcagaa ctcgaggacc
     7381 tgctgctcgg tcagcgccgg gtccctcttc acgatgaaga gcttgggcac ctcgcccgaa
     7441 tgctcgtcgg gcacgccgat cgcagccacc tcgagcacgc ccgggtgcat cgccaccacc
     7501 tgctcgatct cgttcgggta cacgttgaag cccgagacga ggatcatgtc cttcttgcgg
     7561 tcgacgatgc gcacgaaccc ctgcgggtcc atcacgccga cgtcgcccgt gtgaagccac
     7621 ccgtccttgt cgagcacctt cgcggtctcg tcggggcgct gccagtagcc cttcatcacc
     7681 tgcgggccct tgatgcagat ctcgcctggc tggccgagcg gcaccggctt gttgtcgtcg
     7741 tcgcggatct cgaaatcggt cgaaggcacc ggcaggccga tcgagccgtt gaactgcgtg
     7801 aggttgggcg ggttcatcgc ggccaccggg ctggtttcgg tgaggccgta gccctcgagg
     7861 atcaccttgc cggtcacctg cttccagcgc tcggccaccg ccttctgcag cgccatgccg
     7921 ccggcgccgc acactttcac gtgcgagaaa tcgagcttgt ggaactcggg gttgttgagg
     7981 aacgcgttga acagcgtgtt cacgccggtg atcatgttgt agcggtgctt gccgagctcc
     8041 ttcacgaacg cgggaatgtc gcgcgggttg gtgatgagga tgttgaagcc gcccatcttc
     8101 gccatcagca ggcagttcac cgtgagcgag tagatgtggt agagcggcag cgcggtgatg
     8161 atcaccggcg tcgtgccgcc cagcgccgcg gtcaggttcc acgaggactg ctcgatgttg
     8221 gcgaggatgt tctcgtgcgt gagcatcgcg cccttggaca cgcccgtggt gccgccggtg
     8281 tactgcagga aggcgaggtc gccctgcccg accacgaagg gcttcatcgc gaggccggcg
     8341 ccctgcttca gcaccgcatt gaagcgctcg gcgcccggca gctcgaacgc gggcaccatc
     8401 ttcttcacgt tgcgcaccac gaaattgacg atgtggcctt tcacgccgag caggtcgccg
     8461 agcgccgcca ccgcgatgtg cttcacgggc gagcgcggca gcacctcctc cagcgtgtgg
     8521 gcgaagttct cgaggatgac gatggcctcg gcgccggagt ccttcaactg gtgctcgagc
     8581 tcgcgcgccg tgtacagcgg gttgacgttg accacggtgt agccggcgcg cagcgtgcca
     8641 aagagcacga tcggatactg caggcaattg ggcatcatga tggcgacgcg cgcgcccttc
     8701 gccagccctt tggactgcag ccaggcggcg aagtcgcggc tcagcttgtc gatctcgccg
     8761 aagctgagcg tcttgcccat gcagtggaac gcgggctggt cggcgaactt cctgcagctc
     8821 gtctcgaaca gctcgcccac cgacttgaac tcgttccagg tcacgtcggc gggcatgccg
     8881 gggggatagc tcttcagcca gatgcgttcc atggggtcat cctcctcgag gcagatgata
     8941 ctagcgtgcg gcccgcaata cccgcaccac gagcggcgcg gcgaggcagc cggcgccgat
     9001 ggccgcgtac ccgcaggccc aggcgaggag gctcgcctgc ccgccggcaa tgtcgagcgc
     9061 cgcgccgaac accgccggcc cgagcatgcc gccgccgaag ccgacgagcg agtaaaggcc
     9121 catggccgcc ccgcgcagct cgggcggagc ggccgccacg taccccgccg tgagcgtggc
     9181 cgactcggcc atgatgagca tggaatagac catcagcagc gccaccacga acgcccagtg
     9241 cagcggcgcc gagaacgcga gtccgatccc cgtgagcgac gatgcggtca tgacgatgag
     9301 gatccagcgc cgccggccga tgcggagcgc catttcgttg ccgaggatcg acgccggcac
     9361 ggcggccgcg ttcgcgactg cggcgatcgc ggccggcccc cagggcgcgc cgcccgcgac
     9421 cccgagtccc gcggcaaacg agagaaacgc caccatccag ccgcgcgagc cgaagagctc
     9481 caggcagtgc acgccgtagc cgatcatgta gccggctgcg gcgcggttgg cgagcacctt
     9541 gcgccaggcc gcgagcggaa acaggatcga atgcgtgctg ccggcgggct ctttcgccgg
     9601 caggctcgcg aacaacatga cgccggaaac gacggagccc gcggcgcaca gcacgaacgc
     9661 cgcgcgccag ccccacagcg gcgcgatgaa gcccgccagc gcaaaggaga tcgccgcgcc
     9721 aatgccgaag aaggacgtat agaaggcgat gtagcggctt tgcgccgggc cgtgcacgac
     9781 gtccgacagc agccgcaggc ccggcatgta ggtgccggcg atgccggcgc caaggagcgc
     9841 ctggaacgcg caggcgctca ggaagccctg cgcaagcaga ccgaaaccgg cgcagcccgc
     9901 gaacgcgagc gcgctgccgg ccgcgtaaac gtagcgcccg tccacgcggt cggtgagcgc
     9961 cgtccaggcg gacaccgtgg cgatgtagcc ggcaaagaac aggctggcga tgatcccggc
    10021 ttcggagttc gagatctgcc actcgccctg cagtccgggc aacagcgccg gatacgtcga
    10081 aaagcccagc atcgaaaaga tgtgggcggc gatgagaata atggggacag acctcatttt
    10141 tcccatttgc acctgattcg cagatgggaa aaatgaggtc tgtccccatt taacgctatt
    10201 tgttcttgaa ggacggcttt ctcttttcca cgaaggcggc catgccttct ttctggtctt
    10261 cgagcgcgaa catggagtgg aacaggcgcc gctcgaagag cacgccctcg gcaaggccgc
    10321 cctcgtaggc gcggttgacc gattccttcg ccatcatcac cgcgggcagc gagtagccgg
    10381 cgattttctc cgccatcgcc atcgcctcct ccatcagctt cgccgccggc acgacgcgcg
    10441 acaccagccc cgcgcgcagc gcctcgtccg cgccgatcat gcgcgcggtg aggatcatgt
    10501 ccatggcgag cgccttcgac gtgaagcgcg gcaggcgctg cgtgccgccc gagccgggca
    10561 tcacgccgag gttgatctcg ggctggccga acttcgccgt atcggcggcg atcgccaggt
    10621 cgcacatcat ggcgagctcg cagccgccgc cgagcgcgaa cccggccacc gcggcgagca
    10681 ccggcttcct gatgttcctg atgtgctccc agtcgcgcgt gatgtagttc gacctgaagg
    10741 catccatgta gccgaagtcc ttcatcgccg cgatgtcggc gccggcggcg aaggccttat
    10801 cgttccccgt gatgacgatg cagccgacgc cgtcgtcggc gtcgaaggca tacagcgcct
    10861 gggcgagctc ctgcatcagc tgcgcgttca gcgcgttcat ctgctttggc cggttgagcg
    10921 tcaccacgcc cacgcgaccc cgggtttcca cgacgatgaa ttcagtgctc atttggatcg
    10981 tgccttcatt tcggaaaggg gaattgcgcc cgggatgtcg atgcgcttgc ggcccggctc
    11041 cacggcggtg agcgcttcga cccgccgcat ggtcttcgca ctgcgctcgg tgagcttctg
    11101 gtacaggtgc atgttgtccg tcttccacgc gagctcctct gccgtcagct cgcgcagcac
    11161 gcgcgccggg atgcccgcgg cgagcccgcg cgcgggaatc ttcgcctcgg ccttgacgaa
    11221 cgccagcgcc gccaccatcg cctcctcgcc cacctcggcg ttgtcgttca ccaccgcgtt
    11281 catgccgacg agcgcaccgc gccggatgac gcagccgtgc agcaccgcgc cgtggccgat
    11341 ggtggcttcg cgcccgacca ccgtgtcctt gcccggaaag ccgtgcagca tgcaggtgtc
    11401 ctggatgttc gcgccttctt ccaccacgat gcggccgaag tcgccgcgca ggcaggcgcc
    11461 cgggccgacg taggcgcgcg caccgacgat cacgtcgccc accagcaccg cgctctcgtg
    11521 cacgaacgcg gacgggtcga ccaccggcac gagtccgtcg atggagtaca cctggggcat
    11581 gccccggatt ctagcgccgc tggtgtaagc tttcgccatg cctccggtcc tcgtttcgac
    11641 ggccgacggc gtgatgacca tcacgctgaa ccgccccgag aagctcaacg ccttcgtgcg
    11701 cgacatgcac gggctgttgc gcgaggcgat ggcgcaggcc gagggcgacg ctgcgatccg
    11761 cgcgctcctt ctcaccggcg cgggccgcgg cttttgcgcc gggcaggacc tcgccgagcg
    11821 cgacatgaac gacccgaacc tcgacctcgg cggcgggctc gaggcgagct acaacccgct
    11881 catccggcgc ctgcgcgcgc tgcccaagcc cgtggtgtgc gccgtcaacg gcatcgccgc
    11941 cggcgcgggc gccaacatcg cgctcgcgtg cgatctggtc ctcgccgcgc gctcggccgc
    12001 cttcatccag gctttctcga agatcgcgct ggtgcccgac tgcggcggca gctactggct
    12061 gccgcgcctg gtcgggctgc agcgcgcgat gggcatggcg ctccttgccg agcgcgtgtc
    12121 ggccgaagac gccgagcgct ggggcctcgt ctggaagtgc gtggacgacg ccaagctcat
    12181 ggacgaggcg acctcgctcg cgcgcacgct cgccgcgggc ccgacgcaca cctacggcat
    12241 gatcaagaag gcgctctacg cttcgagcgg caactcgctc gatcagcagc tcgacctcga
    12301 gcgcgacttc cagcgcgagg tgggccgcca cggcgactac cgcgaaggcg tcgccgcctt
    12361 cctcgagaag cgccaggcca ggttcacggg gcgctgatgg ccaaggcgaa gaagtttccg
    12421 ctcgaaccga tcgagaaggg atcgcgcaag agcctgcgcg agctgcagct gcggcgcctg
    12481 aagtggtcgc tcaagcacgc ctacgacaac gtcccggcgt atcgcaggaa gttcgacgcc
    12541 gcgggcgtga agcccaagga cctgaagtcg ctcgcggatc tctcgaagtt ccccttcacg
    12601 gcgaaggccg acctgcgcga gaactacccc ttcggcatgt tcgccgtgcc gatggaccgg
    12661 atcgtgcgca tccacgcctc ttccggcacc accggcaagc ccacggtcgt gggctacacg
    12721 caaaaggaca tcgatacctg gtcgcacgtg atggcgcgct cgttccgcgc cgcgggcgcg
    12781 cggcccggcg acaaggtgca caacgcctac ggctacgggc tcttcaccgg gggcctcggc
    12841 gcgcactacg gcgcggaaaa gctcggcctc gcggtgatcc cgctctcggg cggcatgacc
    12901 gagcggcagg tccagctgat ccaggacttc aagcccgaca tcatcacggt gacgccttcg
    12961 tacatgctgg cgatcgccga cgagatggaa aagcagggca tcgacccgaa gaaggtgtcg
    13021 ctgcgcatcg gcatgttcgg cgccgagccc tggacccacg agatgcgcaa gcagatcgag
    13081 gcgcgcacct cgctcgacgc gatcgacatc tacgggctgt cggaagtgat cgggccgggc
    13141 gtggcgcagg agtgcatcga gaccaaggac gggctcacgg tctgggagga ccacttctat
    13201 cccgagatcg tcgacccgca atcgggcgcg gtgctgcccg agggcgagca gggcgagctg
    13261 gtgtttacct cgctgaccaa ggaagcgatg ccggtgatcc gctaccgcac gcgcgacctg
    13321 acgcggctgc ttccccccac ggcgcgcagc atgcgccgga tggaaaagat caccgggcgc
    13381 tcggacgaca tgctgatcat ccgcggcgtg aacgtgttcc cgacgcagat cgaggagctg
    13441 atcctcaagc agcccgagct tgcgccgcac tatgtgctcg agctctcgaa ggacgggccg
    13501 atggaccatc tcaccgtgct ggtcgagctc gcgccgggcg ccgcggacgc cgactcggcg
    13561 gcgaagctca agcaccacgt caagtcctac atcggcgtga cggccgacgt gaagatcgtg
    13621 ccgatctcca ccatcgagcg ctcgatcggc aaggccaagc gcgtggtgga caagcgggcg
    13681 aagtgatcct cggcctcacc cggaaggagc tcgaagacgg cgcgcggctc gtgcacgagg
    13741 ccgtgccgcc cacgccgcag tacagctggc cgctgctcaa tgagcgcgcg ggcaccgaag
    13801 tgtgggtgaa gcacgagaac cacaccgcga ccggcgcctt caaggtgcgc ggcgggctgg
    13861 tgctggcgca ggagatcaag cgccgggatc cgaaagtggc aggcgtgatc agcgcgacgc
    13921 gcggcaacca cggccagtcg gtggcctttg ccgggcggcg ccacggcatg cgctcggtga
    13981 tcgtggtgcc gcacggcaac agccgggaaa agaacgccgc catgcgcgcc tggggtgccg
    14041 agctgatcga gcacgggcgc gactacgacg aggcgcgcga gcacgcggcg aagctcgcca
    14101 aggccgaagg gctgttctac atcgggccgt tccaccgcga gctcgtcgtc ggcgtcggca
    14161 gctacgccat ggagctcttt ctcggcgcgc cgcagctcga tgaagtctac gtgccgatcg
    14221 gctgcggctc gggcatctgc ggcgtcatcg cctggcgcga cgcgctcggc ctgaagacgc
    14281 gcgtggtggg cgtggtgtcg gagaacgcag cttgctacgc acactcattc gagtccggcc
    14341 ggccggtgga aacgaagacc gcgaacacgt tcgccgacgg cgttgctgtg cgcgtgccgg
    14401 tggctgaggc gctcgaggcg attctcgccg gcgccgatcg catcgtgcag gtgagcgacg
    14461 acgaagtgaa ggcggcgatg cgcgacttct accgctgcac gcacaacctc gccgagggcg
    14521 ccggcgcggc gccgctcgcc gcgctcatga aggacggcgc gcgaaagggc tcgcgcttcg
    14581 cggtaatcct ctcgggcggg aacatcgacc gggagatgta cctggaggcg ctcggcggga
    14641 acgcaacatc cggatagatt tgccccgagc gccccgctac agtgcggcca tggaaaacgt
    14701 cattgcccga gcgcgcagct acctggacga gcaccgtggc gagcgcgtca cgctcagcga
    14761 tctcgcgcgc gccgtcggcg caagcccgtt ccacctgcaa cgccgcttca agcaggcctt
    14821 cggtctgtcg cccaaggact accagcgcga agc
//
LOCUS       DBNHKE010000092         6243 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_68273, whole genome shotgun sequence.
ACCESSION   DBNHKE010000092 DBNHKE010000000
VERSION     DBNHKE010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_68273"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16O9_05760"
     CDS             complement(<1..367)
                     /locus_tag="AC16O9_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase"
                     /protein_id="HIR6516157.1"
                     /translation="MLLTAPGVLKPDEIALAREWLASVRFVDGKASAGAAARRVKKNL
                     EAERGAGLERLDEMVMGNLARHPAYRGGALPLHAATPLYVRYAPGMQYGEHLDDPIMG
                     AGGVMYRSDVAVTLFLSAPE"
     gene            complement(374..967)
                     /locus_tag="AC16O9_05765"
     CDS             complement(374..967)
                     /locus_tag="AC16O9_05765"
                     /inference="COORDINATES: protein motif:HMM:NF025024.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HIR6516158.1"
                     /translation="MAFENTRIRELKPLSFIYELPRALPADLCREAIRRFEASAAQHL
                     PGRIGAAAEEAPQVKRSTDLRLSGREDWQDIDQALARQLAAVVGAFAREFPFFAANSF
                     KDLGYNLQRTRPGEYYHWHVDGGPGEFGARQLVAIWYLNDVPGPGGETEFPLQEVAVR
                     PEEGKLLLFPPFWTHVHRGVTLREGVKYIATTWICFA"
     gene            complement(971..1294)
                     /locus_tag="AC16O9_05770"
     CDS             complement(971..1294)
                     /locus_tag="AC16O9_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516159.1"
                     /translation="MRFKVIVGEQAYAVEVPERLLEQAREFHDKLDADMDRGWQMSRE
                     FVAHPDRLQRCQIAADRLLTGLMQGNEATAMLMASYIALRMPGVVGVDIDAGGEMRNT
                     ELLYA"
     gene            1344..1541
                     /locus_tag="AC16O9_05775"
     CDS             1344..1541
                     /locus_tag="AC16O9_05775"
                     /inference="COORDINATES: protein motif:HMM:NF049856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Alvin_2107 family globule sulfur oxidation
                     protein"
                     /protein_id="HIR6516160.1"
                     /translation="MDKLYYETIDRMEKKRVDAEYINGWASGFLHNPKREDQRATEAY
                     EAGYAHGMQKNAAGFEAWIRK"
     gene            complement(1531..1971)
                     /locus_tag="AC16O9_05780"
     CDS             complement(1531..1971)
                     /locus_tag="AC16O9_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516161.1"
                     /translation="MSGLDMELASGIAAFEAKEFARAMQLLAPLAERGAPEAQYRLAI
                     MAQVGLGVVKNCAGAVKWMRAAAEQGYALAQHGLAFMYLQGECTKADPAEAAKWFRLA
                     AEQGLAGSQATLASLYEKGVGVAQDAAEAARWRARAEKDAGATS"
     gene            2001..2609
                     /locus_tag="AC16O9_05785"
     CDS             2001..2609
                     /locus_tag="AC16O9_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516162.1"
                     /translation="MAIISSRPQIALVKTRWRKKGERTLAERAGVIGANLWKIAVEIF
                     RHMEREGFRFGSDRMTTEVITEFIAFLVQLVDRAVYGRLAEADRARLVAEVVRHLAAT
                     MENNQMDLFGPGAYRRPFIDALNARFGEYAEFEYRDGAPDYACLRYFASRVADAMVEG
                     DNKWVVEQMIDIVAPEMVRLAAKLVEQTVPAQGNPGPPVAPS"
     gene            complement(2606..3580)
                     /locus_tag="AC16O9_05790"
     CDS             complement(2606..3580)
                     /locus_tag="AC16O9_05790"
                     /inference="COORDINATES: protein motif:HMM:NF045620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sfum_1244 family protein"
                     /protein_id="HIR6516163.1"
                     /translation="MLRNLPELANAVQRNCDISDARYAGDYGMCTFLLKMREYYRWEH
                     ELPLTRALPSAELGDWLSAREARWRALEDAEFEPLPLQSGAADPMAAEAANRELLPRG
                     LVYSAGYGRWHKPVFFLGELLRAEERDGYRVIVSSCEHARELAAPPAMLQGRTIFVRL
                     ESVRRYLWEKVEEWQWRKLDRRMTRVLAGYDFVADPESALARMAENEMESMILHELGE
                     ARAGELLGGAWQDMIASVARTPAEVEARAVRDLLADCLSTLPVLIARENLPALHFYFA
                     TFDAPRRELFPLALEAYEHFLGTGSLERLARVAGEGRERFLAEARALL"
     gene            3690..3905
                     /locus_tag="AC16O9_05795"
     CDS             3690..3905
                     /locus_tag="AC16O9_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6516164.1"
                     /translation="MLDVRGATCPGPVLEAKKLLRRMQAGEVLQLVSDCPGIRSDVHS
                     WVKATGLELADARERAPGEYEFHIRKR"
     gene            3913..4278
                     /locus_tag="AC16O9_05800"
     CDS             3913..4278
                     /locus_tag="AC16O9_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016261.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR6516165.1"
                     /translation="MSLIDEIRAGGAAAAPDPAFPHAPEGWTRARALETATQEGLELG
                     DEHWAAVRALQEYFARHEPTAINLREVHDALDEKFHRQGGIRRLYLLFPGGPVAQGCR
                     IAGLKAPAGATDRGFGSVA"
     gene            complement(4292..4519)
                     /locus_tag="AC16O9_05805"
     CDS             complement(4292..4519)
                     /locus_tag="AC16O9_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6516166.1"
                     /translation="MNFDKELDARGLNCPLPILRAKKALNDMTSGQVLKIVATDPGSV
                     KDFQAFSKQTGNDLLSQSEAEKEFVFFLKRK"
     gene            complement(4554..4829)
                     /locus_tag="AC16O9_05810"
     CDS             complement(4554..4829)
                     /locus_tag="AC16O9_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR6516167.1"
                     /translation="MTRPSMHAPQDKKAMDARLARIEGQVRAVRRMIEADQTCEDIAQ
                     QLAAARKALDRAFYEMVSCMIRSESPTELQARRAQAGRVAELLARFG"
     gene            4978..5574
                     /locus_tag="AC16O9_05815"
     CDS             4978..5574
                     /locus_tag="AC16O9_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR6516168.1"
                     /translation="MSDASDLDRLINERLDALLPKKLAELREKQTPSMTIIATKGTLD
                     WAYPPFILASTAAALGWNVSMFFTFYGLGLLRKDISDLKVSPLGNPGMPMKMPFGPEW
                     FKGINWNIPNAVQAVVPGFENLATTLMKQTIHNKGVAGIEELRSASLEGGVKLIGCQM
                     TVDLFGYTRDDFIPEVKDYVGAATFLPMARDADVSLFI"
     gene            5603..>6243
                     /locus_tag="AC16O9_05820"
     CDS             5603..>6243
                     /locus_tag="AC16O9_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR6516169.1"
                     /translation="MIRKLLLVAAALATATPGAALATTGYFAHGYGIKAKATGGVGIA
                     LPQDALAPASNPAGIAWIGNRIDFGADWFSPDRGASIAGNGFGADGTYDGNDTKSFLI
                     PEFGYSRAINPNLTFGVAVYGNGGMNTDYKNNPFAAFGGTGPAGVDLMQLFVAPTLAW
                     KSGNHAFGVSLNLAYQRFKAEGLQPFDNSPTSSSPGNVTNRGYDDSTGFGVRVG"
BASE COUNT         1011 a         2181 c         2105 g          946 t
ORIGIN      
        1 cctcgggcgc gctgagaaag agcgtcaccg cgacgtccga gcggtacatg acgccgcccg
       61 cgcccatgat cgggtcgtcg aggtgctcgc cgtactgcat gccgggcgca tagcgcacgt
      121 aaagcggcgt cgcggcgtgc agcggcagcg cgccgccgcg gtacgcggga tggcgcgcga
      181 ggttgcccat caccatctcg tcgagccgct cgagcccggc gccgcgctcg gcctcgaggt
      241 tcttcttcac gcgccgcgcc gccgcgcccg ccgaggcctt gccgtccacg aagcgcacgc
      301 tcgcgagcca ctcgcgcgcg agcgcgatct cgtcgggctt cagcacgccg ggcgcggtga
      361 gcagcatgcg gcctcaggcg aagcagatcc aggtggtggc gatgtatttc acgccctcgc
      421 gcagcgtgac gccgcggtgc acgtgcgtcc agaacggcgg aaagagcagc agcttgccct
      481 cctccggccg caccgcgacc tcctgcagcg ggaactcggt ctcgccgccc ggtcccggca
      541 cgtcgttcag gtaccagatc gccaccagct ggcgcgcgcc gaactcgccc ggtcccccgt
      601 ccacgtgcca atggtagtac tcgccgggcc gcgtgcgctg caggttgtag cccaggtcct
      661 tgaacgaatt ggcggcgaag aacgggaact cgcgcgcgaa ggcgccgacc acggcggcga
      721 gctgccgggc gagcgcttgg tcgatgtcct gccagtcctc gcgcccgctg aggcgcaggt
      781 cggtggagcg cttgacctgc ggcgcctcct cggccgccgc gccgatgcgc ccgggcaggt
      841 gctgcgcggc gctcgcctcg aagcgccgga tcgcctcgcg gcagaggtcg gcgggcagcg
      901 cgcgcggcag ctcgtagatg aagctgagcg gcttcagctc gcggatacgg gtgttctcga
      961 acgccatcgc ctaggcgtac agcagctccg tgttgcgcat ctcgcccccg gcgtcgatgt
     1021 ccacgcccac cacgcccggc atgcgcagcg cgatgtatga ggccataagc atcgccgtgg
     1081 cctcgttgcc ctgcatgagg ccggtgagca gccggtcggc agcgatctgg cagcgctgca
     1141 gccggtcggg gtgcgcgacg aactcgcggc tcatctgcca gccgcggtcc atgtcggcat
     1201 ccagcttgtc gtggaactcg cgcgcctgct cgagcaggcg ctccggcacc tccaccgcgt
     1261 aggcctgctc gccaacgatc accttgaaac gcatgcgcct tttattacca tatgcggacc
     1321 gcaaaacctg gaaatccaaa cccatggaca agctctacta cgagaccatc gaccgcatgg
     1381 agaagaagcg ggtcgatgcg gagtacatca acggctgggc ctcgggcttc ctgcacaacc
     1441 ccaagcgcga ggaccagcgc gccaccgaag cctacgaggc gggctacgcg cacggcatgc
     1501 agaagaacgc cgcgggcttc gaggcctgga tcaggaagta gcgcccgcgt ccttctcggc
     1561 gcgcgcgcgc cacctggcgg cctcggccgc gtcctgcgcc acgccgacgc ccttctcgta
     1621 cagcgacgcg agcgtcgcct gcgagcccgc caggccctgc tcggcggcga ggcgaaacca
     1681 cttcgccgcc tccgccggat cggccttggt gcactcgccc tgcaggtaca tgaacgcgag
     1741 cccgtgctgc gcgagggcgt agccctgctc ggccgcggcg cgcatccatt tcacggcgcc
     1801 ggcgcagttc ttcaccacgc ccaggcccac ctgcgccatg atggcgaggc ggtactgcgc
     1861 ctcgggcgcg ccgcgctcgg cgagcggcgc gagcagctgc atggcgcgcg cgaactcctt
     1921 cgcctcgaag gcggcgatgc cgctcgccag ctccatatcc agtcctgaca tcaggtcctc
     1981 ccgcgtctat tatcccccgc atggccatca tcagcagccg tccgcaaatt gcgctcgtca
     2041 agacgcgctg gcgcaaaaag ggcgagcgca cgctcgccga gcgcgccggc gtgatcggcg
     2101 ccaacctctg gaagatcgcg gtggagattt tccgccacat ggagcgggag ggctttcgct
     2161 tcggctcgga ccgcatgacg accgaggtga tcacggagtt catcgccttc ctggtccagc
     2221 tcgtcgaccg cgcggtgtac ggcaggcttg ccgaggccga ccgcgcgcgc ctggtcgccg
     2281 aggtggtgcg ccacctcgcc gcgaccatgg agaacaacca gatggacctg ttcgggcccg
     2341 gcgcgtaccg ccggcccttc atcgacgcgc tgaacgcgcg ctttggcgag tacgccgagt
     2401 tcgaataccg cgacggcgcg ccggactatg cgtgcctgcg ctactttgcc agccgcgtcg
     2461 ccgacgccat ggtcgagggc gacaacaagt gggtggtcga gcagatgatc gatatcgtgg
     2521 cgccggaaat ggtgcgcctg gcggcgaagc tggtcgagca gacggttccg gcacagggga
     2581 acccgggtcc ccccgtggcc ccttcctaaa ggagagcgcg cgcctcggcc aggaagcgct
     2641 cccggccttc gccggcgacg cgcgccagcc gctcgagcga gccggtgccg aggaagtgct
     2701 cgtaggcctc gagcgccagc ggaaacagct cgcgccgcgg cgcatcgaag gtggcgaagt
     2761 agaaatgcag cgccggcagg ttctcgcgcg cgatgagcac gggcagcgtc gacaggcagt
     2821 cggcgagcag gtcgcgcacc gcgcgcgcct cgacctcggc gggcgtgcgc gccaccgagg
     2881 cgatcatgtc ctgccacgcc ccgcccagca gctcgcccgc gcgcgcttcg ccgagctcgt
     2941 gcaggatcat gctttccatc tcgttctcgg ccatgcgtgc gagcgcgctt tccgggtcgg
     3001 cgacgaagtc gtagcccgcg agcacccggg tcatgcgccg gtcgagcttg cgccactgcc
     3061 attcctcgac cttctcccac agatagcgcc gcaccgactc caggcgcacg aagatggtgc
     3121 gcccctgcag catcgccggc ggcgcggcaa gctcgcgcgc gtgctcgcag gacgaaacga
     3181 tcacccgata gccgtcccgc tcctccgcgc gcagcagctc gccgaggaag aacaccggct
     3241 tgtgccagcg cccgtagccc gcgctgtaga ccaggccccg gggcaggagc tcgcggttgg
     3301 cggcctcggc cgccatcgga tccgcggcgc cactctgcag cggcagcggc tcgaactccg
     3361 cgtcctccag cgcgcgccag cgcgcttcgc gcgcgctcag ccagtcgccc agctccgcgc
     3421 tgggcagcgc gcgcgtgagc ggcagctcgt gctcccagcg gtagtactcg cgcatcttga
     3481 gcaggaaagt gcacatcccg tagtcgccgg cgtagcgcgc atcggagatg tcgcagttgc
     3541 gctgcaccgc gttggccagc tcggggagat tgcgcagcat catggcgcgt atatactgac
     3601 tttagcatcg acgcgcatgg ccagaaccgc caaaccgaaa gcccgcgtca cgctcgacat
     3661 gaagggcgcg gcgggtcacg ccgcacgccg tgctcgacgt gcgcggcgcg acctgccccg
     3721 gccccgtgct cgaggcgaag aagctgctcc ggcgcatgca ggcgggcgaa gtgctgcagc
     3781 tggtgagcga ctgcccgggg atacgctcgg acgtgcacag ctgggtgaag gccaccgggc
     3841 tcgagctcgc cgatgcgcgc gagcgcgccc cgggcgagta cgagttccac atccgcaagc
     3901 gctgaggacg acatgagcct gatcgacgag atccgggcgg gaggcgccgc tgccgcgccg
     3961 gacccggcgt ttccgcacgc gccggagggc tggacgcgcg cgcgcgcgct ggaaaccgcg
     4021 acgcaggaag gactcgagct cggcgacgag cattgggcgg cggtgcgcgc gctgcaggag
     4081 tacttcgcgc ggcacgagcc caccgcgatc aacctgcgcg aggtgcacga cgcgctggac
     4141 gagaaattcc accgccaggg cgggatccgc cgcctgtatc tgctgtttcc gggcgggccg
     4201 gtggctcagg gctgccgcat cgcgggactc aaggcgcccg ccggcgcgac cgaccggggc
     4261 ttcggcagcg tggcctgagg cgcggcgcgc gctacttgcg cttcaggaag aaaacgaact
     4321 ctttttcggc ctcggactgc gacagcaggt cgttgccggt ctgcttggaa aacgcctgga
     4381 agtccttcac cgagccgggg tcggtggcga cgatcttgag cacctgcccc gaggtcatgt
     4441 cgttgagcgc cttcttggcg cgcaggatgg gaagcgggca attcagcccg cgggcgtcga
     4501 gttctttgtc gaagttcatg ggcgaatcct aaggtttccg ggggtcgaag tcatcagccg
     4561 aagcgcgcca gcagctcggc cacgcgcccg gcctgcgccc ggcgcgcctg cagctccgtg
     4621 ggcgattcgc tgcggatcat gcaggacacc atctcgtaga acgcgcggtc gagcgccttg
     4681 cgcgccgccg cgagctgctg cgcgatgtcc tcgcaggtct ggtcggcctc gatcatgcgg
     4741 cgcaccgccc gcacctgtcc ctcgatgcgc gccaggcggg catccatcgc cttcttgtcc
     4801 tgcggggcgt gcatgctggg acgcgtcatg cgcggcaata tacgccaggg ggtatggtgg
     4861 cctcaacttt cggtgttagt gcgccgcacc ataagcgatt cctgcgaccc catcagagga
     4921 acaatctggc gcatactccg gcccagtatt agcgtcccac ttctcaagga gccccgcatg
     4981 tccgacgcat ccgatctcga tcgcctgatc aacgagcgcc tcgacgcgct gctgcccaag
     5041 aagctcgccg agctgcgcga gaagcagacg ccctcgatga ccatcatcgc caccaagggc
     5101 acgctcgact gggcctaccc gccgttcatc ctcgccagca ccgcggccgc gctcggctgg
     5161 aacgtatcca tgttcttcac cttctacggg ctggggctgc tgcgcaagga catcagcgac
     5221 ctcaaggtga gcccgctcgg caacccgggc atgccgatga agatgccctt cgggccggag
     5281 tggttcaagg gcatcaactg gaacattccc aatgccgtgc aggcggtcgt gccgggtttc
     5341 gagaacctgg cgacgacgct catgaagcag accatccaca acaagggcgt ggccggcatc
     5401 gaagagctgc gcagcgccag cctcgagggc ggcgtgaagc tgatcggctg ccagatgacg
     5461 gtggacctgt tcggctacac gcgcgatgac ttcattccgg aagtaaagga ctacgtgggc
     5521 gccgccacct tcctgccgat ggcgcgcgac gccgacgtga gtctgttcat ttgagcgggt
     5581 tccactaaca ggagagggaa agatgatcag gaagctgctg ctcgttgcgg cggcgctcgc
     5641 cacggccacg ccgggcgccg cgctggcgac gaccgggtat ttcgcgcatg gctacggcat
     5701 caaggcaaaa gcgacgggcg gcgtgggcat cgccttgccg caggacgcat tggcgccggc
     5761 gagcaatccg gcgggcattg cgtggatcgg caaccgcatc gatttcggtg ccgactggtt
     5821 cagtccggac cggggcgcgt cgattgccgg caatggattt ggtgcagacg ggacctacga
     5881 cggcaacgac acgaagagct ttctcattcc ggagttcggc tacagccgcg ccatcaaccc
     5941 gaacctgacc ttcggggtag ccgtctacgg caacggcggg atgaacaccg attacaagaa
     6001 caatccattc gctgcctttg gcgggacggg gccggccggc gtcgacctca tgcagctttt
     6061 cgtcgcgccg acgctcgcct ggaaaagcgg gaaccatgcc ttcggcgtgt cactcaacct
     6121 tgcctaccag cgcttcaagg ccgaagggct gcagccgttc gacaactcac ctacgtcttc
     6181 gagccccggc aatgtaacca accgcggcta cgacgactct acgggtttcg gcgtgcgcgt
     6241 ggg
//
LOCUS       DBNHKE010000093         8711 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1179309, whole genome shotgun sequence.
ACCESSION   DBNHKE010000093 DBNHKE010000000
VERSION     DBNHKE010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1179309"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="AC16O9_05825"
     CDS             <1..639
                     /locus_tag="AC16O9_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6516170.1"
                     /translation="DLEGSALGLALDALLQRRADGWHALVPLRAPASGPSALRIDAAQ
                     VREALLGSGALFIDLKAEANALYAGYLNEAIALAAAGIGAVLLLLMLALRSAARVGRV
                     AAPLGAAVLVAAAALAAAGQTLNLLHLVGFLLVVAVGSNYALFFERAGGAPAPRVLAS
                     IVLANATTVIGFGVLATSSVPVLRAIGATVALGTLLALAFAALLSGAHGDAR"
     gene            647..1462
                     /locus_tag="AC16O9_05830"
     CDS             647..1462
                     /locus_tag="AC16O9_05830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6516171.1"
                     /translation="MTLSNTAAPWRPTRFLRGSAALHAAALAGVAIGPELWPWALGAV
                     AANQAAILAGGLLPRSRLRGPNWTRRPERAAARREVALSLDDGPDPDVTPRVLALLAE
                     RGARATFFCIGERAARHPELVRSIAAAGHAVENHSLRHRHDFALLGPRRYVEEIGSAQ
                     NTLAALAGSAPCFFRAPAGLRNPFLDLALTRLGLQLVSWTRRGYDTRNADAADVLARL
                     ARGLAPGDILLLHDGHAVRDAAGAPVLLEVLPRLLDVLAAAQLRPVTLRAALD"
     gene            1459..2139
                     /locus_tag="AC16O9_05835"
     CDS             1459..2139
                     /locus_tag="AC16O9_05835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516172.1"
                     /translation="MSGDAHYARLVEAASAPYRAAGRFAWHFARGKLGADPVFPYLLQ
                     ARLIPENARLLDLGCGQGLLAALLAAAGTPRLAGYRGIELMARDVARAHRALGAQCGV
                     RQGDIRSAEFGAADAVVILDVLHYMSLAEQDAVLGRVRAALPAGGVLLLRVGDAAAGL
                     PFRLSNWVDWTVAFVRGHGATRFHCRSVAQWRSALEALGFAVHAEPMSRGTPFANVLL
                     SARLPQSH"
     gene            2146..3336
                     /locus_tag="AC16O9_05840"
     CDS             2146..3336
                     /locus_tag="AC16O9_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR6516173.1"
                     /translation="MHPLQLTSFTLTTCLGRGLAPTLRALQGRASGLARRPFESADLD
                     TCIGEVGGVDAVRLAPALAAWDCRNNRLAELGLAQDGFADAVGAAAGRLGAARIGVFL
                     GTSTSGILQTELAYRRRDPATGALPPDFDYAHTHNTYSVTGFVRARLGLAGPAWTVST
                     ACSSSAKVFGTAARMIEAGLIDAAVVGGVDSLCLTTLYGFNSLELLAREPCRPWDRDR
                     SGLSIGEAAAFALVEPLPAARAPDAIVLYGVGESSDAHHMSSPHPEGLGARLAMEAAL
                     GAAGIRPADVGYVNLHGTATPSNDAAEDRAVTDLLGTGVACSSTKGATGHTLGAAGGV
                     EAVIAALALRHGFVPAGANTTEPDPALRANYVLEGRAAPLRHVLSNSFGFGGTNCSLV
                     LGRA"
     gene            3333..4127
                     /locus_tag="AC16O9_05845"
     CDS             3333..4127
                     /locus_tag="AC16O9_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HIR6516174.1"
                     /translation="MSGLAAAVERVALLGPGLSDWAGASAALAGERPYTRAPTVVPAP
                     ALLPPNERRRSGLAVKIALAVGAAALDGYDAARIDTVFASSSADGDTCHAICEQLAGA
                     ERLISPTRFHNSVHNAPSGYWSIATRCMAPSTSLCGYDASFGAGLLEALAFLRAHVRP
                     VLLVAYDAPYPEPLLAKRPVRDAFGVALLLAPRAGAGALAQLELTLTDRAPDELADAG
                     LEALRRAVPTARSLPLLGLLARRERGIARIEYLGGPHLALEVAPCA"
     gene            4118..4549
                     /locus_tag="AC16O9_05850"
     CDS             4118..4549
                     /locus_tag="AC16O9_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApeP family dehydratase"
                     /protein_id="HIR6516175.1"
                     /translation="MRVGHDWIAAHIPHQGAMCLLQEVLEWDAQRIVCATSSHRDPAN
                     PLRVAGTLPAACGIEYGAQAMAVHGALLEAQGAPLSRGFLASVRSVTLHTARLDDVAG
                     PLRVSAERLSGEGDHILYAFSVACDAGELLGGRAAVVLAAP"
     gene            4546..5268
                     /gene="fabG"
                     /locus_tag="AC16O9_05855"
     CDS             4546..5268
                     /gene="fabG"
                     /locus_tag="AC16O9_05855"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR6516176.1"
                     /translation="MSPIRALVTGGSGGIGAAICRRLAAEGRHVIVHAHRNLEAAHAL
                     VAQIAATGGSAEALAFDVTDAVAARAALEALLERGAVQILVNNAGIHDDAAFPGMSEA
                     QWRSVIDVSLNGFFHVTQPLALPMIRTRWGRIVNMASVSAVAGNRGQANYAAAKGALI
                     SATRSLALELGKRGVTVNAVVPGLIATAMSAEFPPERLRELVPLQRAGSAEEVADLVA
                     FLASERAAYITGQAIAINGGML"
     gene            complement(5249..6013)
                     /locus_tag="AC16O9_05860"
     CDS             complement(5249..6013)
                     /locus_tag="AC16O9_05860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6516177.1"
                     /translation="MSAGAPSRTHLVVIPSYNPGPQVYETVRAARAHWNPVWVVVDGS
                     TDGTRAELERMTRADAGLRVLALERNCGKGAAVLHALRAAAADGYSHVLTMDADGQHP
                     AASIPEFMAASQRAPEAMVLGVPQFDASAPALRVQGRRVSNWWANLETLWCGVQDSLF
                     GFRVYPVRALLEVMQAQRWMRRFDFDPEAAVRLAWRGVPPVNLPAPVRYLRPEEGGVS
                     HFRYWRDNALLTWMHFRLMLGFLARLPRLVVRASRR"
     gene            complement(6010..6798)
                     /locus_tag="AC16O9_05865"
     CDS             complement(6010..6798)
                     /locus_tag="AC16O9_05865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6516178.1"
                     /translation="MILRSLRALWAYAALWYGLALLGLGSLTWSLLAVPLRLLLPARA
                     GRALGRGVIARGFRFYLWALQAVGACRFELGELDRLRGAGPMILAPNHPSLLDAVLLL
                     SRLPQAACVLKAPLAESLVFGAGARLAGYIRNDSPIGMVRHALAELREGGQLLLFPEG
                     TRTTRAPVDAFRGALALIAQQAGVPVQTVFIETDSPFLGKGWPLTRRPPLPLSYRVRL
                     GRRFEPRRDLHAFLRELEQYYQAELAVALEPAPPFPADVESRPA"
     gene            complement(6806..7627)
                     /locus_tag="AC16O9_05870"
     CDS             complement(6806..7627)
                     /locus_tag="AC16O9_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HIR6516179.1"
                     /translation="MKRALWLLCALAAAPAAAEELPLWELGVGVGGFALPDYRGSDQA
                     RGYVLPVPYVVYRGDFLKADRRGIRGELFDTDRVELNISVAASLPVNSSRNDARAGMP
                     DLRPSFELGPSLELALWRSRDGRDLLELRLPLRAAFTLEASPEHIGWVATPNLNLDLA
                     GRGTLAGWNFGLLAGPVYGSRRQHEYFYGVAPQFATPARPTYAAPGGYAGFQFLAAAS
                     RRFAGFWVGAFVRADTLSGAAFEASPLVKRDTYVAAGAGIAWVLGVSRERAEAAE"
     gene            complement(7624..>8711)
                     /locus_tag="AC16O9_05875"
     CDS             complement(7624..>8711)
                     /locus_tag="AC16O9_05875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6516180.1"
                     /translation="WGAELVSPAHPRPTRLAFADAWDKSLPYAYQVRRSQFDEILFRN
                     AARKGALAQEGCRVRGVEFAPGGGACLQIEREDGGAETWRARFVADASGRDTFLGGRL
                     GAKRRNARHNSAAIYAHFRGARRNAGSCEGDITIFWFEHGWFWFIPLADGITSVGAVV
                     WPYYLKSRTKPVNEFLMDTIALCPKLAARLEGAERVTAAEATGNYSYACDRTHGPGYL
                     LLGDAYAFIDPVFSTGVMLAMTSAVDGADAIETCLRRPGKARRALARFDRRTRRGPRE
                     FSWFIYRVTSPAMRGLLMGPRNLLRMREALLALLAGDVFSRSPIGPSLLAFKGLYYFL
                     SLADPRAALRAWRRRRSSLRDAANVGA"
BASE COUNT         1143 a         3314 c         3134 g         1120 t
ORIGIN      
        1 gacctcgaag gcagcgcgct cggcctggcg ctcgatgccc tgctgcagcg gcgcgccgac
       61 ggctggcatg cgctcgtgcc gctgcgcgcg cccgcctcgg gcccgtcggc gctccgcatc
      121 gacgccgccc aggtgcgcga ggcgctcctg ggcagcggtg cgctgttcat cgacctcaag
      181 gccgaggcca atgcgctcta tgccggctac ctgaacgagg ccattgcgct cgccgccgcc
      241 ggcatcggcg ccgttctgct gctgctcatg ctcgcgctgc gctcggcggc gcgcgtcggt
      301 cgcgtggcgg caccgctcgg cgccgcggtg ctggtcgcgg ccgcggcgct cgccgccgcc
      361 ggccagacgc tgaacctgct gcacctggtg ggcttcctgc tggtcgtggc ggtgggctcg
      421 aactacgcgc tgttcttcga gcgcgccggc ggcgcgcctg cgccgcgggt gctggcttcg
      481 atcgtgctgg ccaatgccac cacggtgatc ggctttggcg tactcgccac ctcgagcgtg
      541 ccggtgctgc gcgccatcgg cgcgaccgtc gcgctcggca cgctgctcgc gctcgccttc
      601 gcggcgttgc tctcgggcgc gcacggcgat gcgcgataat cgcgccatga ccttgtcgaa
      661 caccgccgcg ccctggcgcc cgacgcgctt tctgcgcggc tcggccgccc tgcacgccgc
      721 ggcgctggcc ggcgtcgcca tcggccccga gctctggcct tgggcgctcg gcgcggtcgc
      781 cgccaaccag gcggcgatcc tcgccggcgg gctgctgccg cgcagccggc tgcgcggacc
      841 aaactggacg cgccggccgg agcgcgccgc ggcgcgtcgc gaggtcgcgc tttcgctcga
      901 cgatggcccc gatccggacg ttacgccgcg cgtgctggcg ctgctcgccg agcgcggcgc
      961 ccgggcgacg ttcttctgca tcggcgagcg tgccgcgcgc catcccgaac tggtgcgctc
     1021 gatcgccgcg gcgggccacg cggtggaaaa ccactcgctg cgccaccgcc acgacttcgc
     1081 cctgctcggg ccgcgccgct acgtcgagga gatcggcagc gcgcagaaca cgctcgcggc
     1141 gcttgccggc agcgcgccgt gcttctttcg cgcgccggcc ggcctgcgca atccgttcct
     1201 cgatctcgcg ctcacgcgtc tcggcctgca gctggtgagc tggacgcgcc gcggctacga
     1261 cacccgcaac gccgacgccg cggacgtcct cgcacggctc gcgcgcggcc ttgcgcccgg
     1321 cgacatcctg ctgctgcacg acggccacgc ggtgcgcgac gcagccggcg cgccggtgct
     1381 gctcgaggtg ctgccgcggc tgctcgacgt actggccgcg gcccaactgc ggccggtcac
     1441 gctgcgcgcg gcgctcgatt gagcggcgac gcgcactacg cccgcctggt cgaggcggcg
     1501 agcgcgccgt accgcgccgc cgggcgcttc gcctggcact tcgcgcgcgg caagcttggg
     1561 gccgacccgg tgttcccgta ccttctgcag gcgcgcctga tcccggagaa cgcgcgcctg
     1621 ctcgacctgg gctgcggcca gggcctgctc gcggcgctgc tcgccgccgc gggcacgccg
     1681 cgcctcgccg gctatcgcgg catcgagctg atggcgcgcg acgtagcgcg cgcgcatcgc
     1741 gccctgggcg cgcagtgcgg cgtgcggcaa ggggacatcc gcagcgccga gttcggcgcc
     1801 gccgacgcgg tcgtgatcct cgacgtgctg cattacatga gcctcgccga gcaggacgcg
     1861 gtgctcggcc gcgtgcgcgc ggcgctgccg gcgggcggcg tgctgctgct gcgcgtgggc
     1921 gacgcggctg ccggcctgcc gtttcgactc agcaactggg tcgactggac cgtggccttc
     1981 gtgcgcggcc acggcgccac gcgattccac tgccgcagcg tggcacaatg gcgctcggcg
     2041 ctggaagcgc tcggcttcgc cgtgcacgcc gagccgatga gccgcggtac gccgttcgcc
     2101 aacgtgctgc tctcggcgcg cctgccgcaa tcccactgac gcgacgtgca cccgctgcaa
     2161 ctcacgagct tcaccctcac cacctgcctg ggccggggcc tggcgccgac gctgcgcgcc
     2221 ctgcagggcc gtgcgagcgg cctggcgcgc cgccccttcg agagcgcgga cctcgacacc
     2281 tgcatcggcg aggttggggg cgtggacgca gtgcggcttg cacccgcgct cgccgcctgg
     2341 gactgccgca acaaccgttt ggccgagctc ggcctcgcgc aggacggatt tgccgacgcg
     2401 gtcggcgcgg ccgcggggcg gctgggcgcg gcccgcatcg gcgtgttcct cggcaccagc
     2461 acctcgggca tcctgcagac cgagctcgcc taccgccgcc gcgatcccgc aaccggcgcg
     2521 ctgccgccgg atttcgacta cgcgcacacc cacaacacct actcggtcac gggattcgtg
     2581 cgcgcccggc tcgggctcgc cgggccggcg tggaccgtgt ccaccgcctg ctcctcgagc
     2641 gccaaggtat tcggtacggc ggcgcgcatg atcgaggcgg gcctgatcga cgccgcggtg
     2701 gtcggcggcg tcgacagcct gtgcctcacc acgttgtacg gcttcaactc gctcgagctc
     2761 ctcgcgcgcg agccgtgccg cccctgggac cgggaccgca gcggcctgtc gatcggcgag
     2821 gcggcggcgt ttgccctggt ggagccgctg ccggcagcgc gcgcgccgga tgccatcgtg
     2881 ctgtacggcg tcggcgaatc gagcgacgcg caccacatgt cctcgccgca ccccgagggc
     2941 ctgggcgcgc gcctcgccat ggaagcggcg ctcggcgccg ccggaatccg gcccgccgac
     3001 gtcggctacg tcaacctgca cggcacggcg accccgagca acgacgccgc cgaggaccgc
     3061 gccgtcaccg atctgctcgg caccggggtc gcatgcagct cgaccaaggg cgcgaccggg
     3121 cacacgctcg gcgccgccgg tggcgtcgaa gcggtgatcg cggcgctcgc cctgcgccac
     3181 ggcttcgttc ccgcgggcgc gaatacgacc gaaccggatc cggcgctgcg cgcgaactac
     3241 gtgcttgagg gccgcgcggc gccgctccgg cacgtgctta gcaactcgtt cggcttcggc
     3301 ggcaccaact gcagcctggt gctgggacgc gcatgagcgg actcgccgcc gccgtggaac
     3361 gcgtcgcgct gctcggaccc ggcctgagcg actgggcggg cgcaagcgcc gcgctcgccg
     3421 gtgaacggcc ctacacccgt gcccccaccg tagtgccggc gcccgcgttg ctgccgccga
     3481 acgagcgccg gcgcagcggc ttggcggtga agatcgccct cgccgtgggc gccgccgcgc
     3541 tggacgggta cgacgcggct cgcatcgaca ccgtgtttgc gtcttcctcc gcggacggcg
     3601 acacctgcca cgccatctgc gagcagctcg cgggcgccga gcgcctgatc tcgccgacgc
     3661 gcttccacaa ctcggtgcac aacgcgccct cgggctactg gagcatcgcg acgcgctgca
     3721 tggcgccctc gaccagcctg tgcggttacg acgcgagctt cggcgccgga ctgctggagg
     3781 cgctcgcctt cctgcgcgcg cacgtccgcc cggtcctgct ggtcgcctac gacgcgccct
     3841 accccgagcc gctgctcgcc aagcggccgg tccgcgacgc tttcggcgtg gcgctgctgc
     3901 tcgccccgcg ggccggcgcg ggcgcgctgg cacagctgga gctgacgctg accgaccgtg
     3961 cgcccgacga gctcgccgac gccggcctcg aagccttgcg ccgcgccgtg cccaccgcgc
     4021 gcagcctgcc gctgctcggc ctactcgcgc ggcgcgagcg cggaatagcg cggatcgagt
     4081 acctcggcgg tccacacctc gcactcgagg tggcgccgtg cgcgtaggcc acgactggat
     4141 cgcggcgcac atcccgcacc agggcgccat gtgcctgctg caggaagtgc tcgagtggga
     4201 cgcgcagcgc atcgtctgcg ccacctcgag ccaccgcgat ccggcgaacc cgctgcgcgt
     4261 ggcgggcacg ctgcccgccg cctgcggcat cgagtacggg gcacaggcga tggcggtgca
     4321 cggcgcgctg ctcgaggcgc aaggcgcgcc gctgtcccgc ggctttctcg ccagcgtgcg
     4381 ctcggtgacg ctgcacaccg cgcgcctcga cgacgtggcc ggccctctgc gcgtcagcgc
     4441 cgagcgcctg agcggcgaag gtgatcacat cctctacgcg ttcagcgtcg cctgcgacgc
     4501 gggcgagttg ctcggcggcc gcgctgccgt ggtgctcgcg gcgccgtgag ccccattcgc
     4561 gccctggtca ccgggggcag cggcggcatc ggcgccgcga tctgccggcg cctcgcagcc
     4621 gaaggccgcc acgtcatcgt gcacgcgcac cgcaacctcg aggccgccca cgcgctcgtc
     4681 gcgcaaatcg ccgccacggg cggcagcgcc gaggcgctcg ccttcgacgt cacggacgcc
     4741 gtggcggcgc gcgccgcgct cgaggcgttg ctcgagcgcg gcgcggtcca gatcctggtg
     4801 aacaacgccg gaatccacga cgatgccgcc ttcccgggca tgagcgaggc gcagtggcgc
     4861 agcgtgatcg acgtctcgct caacggattc ttccacgtca cgcagccgct tgcgctgccg
     4921 atgatccgca cgcgctgggg gcgcatcgtc aacatggcct cggtgagcgc ggtggcgggc
     4981 aaccgcggcc aggccaacta cgctgccgcc aagggagcgc tgatctccgc gacacgctcc
     5041 ctcgccctgg agctgggcaa gcgcggcgtg accgtgaacg cggtggtgcc gggcctgatc
     5101 gcgacggcga tgagcgccga gtttccgccc gagcgcctgc gcgagctggt gccgctgcag
     5161 cgcgccggca gcgccgagga ggtggccgac ctggtcgcct ttctcgcctc ggagcgcgcg
     5221 gcctacatca ccggccaggc gattgcgatc aacggcggga tgctctgacc acgagccgcg
     5281 gcagccgggc gagaaagccg agcatcagcc ggaagtgcat ccacgtcagc agtgcattgt
     5341 cgcgccagta gcggaagtgc gacaccccgc cctcctcggg ccgcaggtag cgcaccggcg
     5401 ccgggagatt gaccggcggt acgccgcgcc aggcgagccg caccgccgcc tcgggatcga
     5461 agtcgaagcg ccgcatccag cgctgcgctt gcatcacctc gagcagcgcg cgcaccggat
     5521 agacgcgaaa accgaacagt gagtcctgca cgccacacca cagcgtctcc aggttcgccc
     5581 accagttgga cacgcgccga ccctgcacgc gcagcgcggg cgcgctggcg tcgaactgcg
     5641 gcacgccgag caccatcgcc tcgggcgcac gctgcgaggc agccatgaat tcagggatcg
     5701 acgccgcggg atgctggcca tccgcgtcca tggtcagaac gtggctgtag ccatcggcgg
     5761 cggccgcgcg cagcgcgtgc aggacagcgg cgcccttgcc gcaattgcgc tccagcgcca
     5821 ggacgcgcag cccggcatcg gcgcgcgtca tgcgctccag ttctgcgcgc gtgccgtccg
     5881 tgctgccgtc caccaccacc cacaccggat tccagtgcgc gcgcgccgcg cgtacggtct
     5941 cgtacacctg cgggccgggg ttgtagctcg ggatgaccac caggtgggtg cgcgacggcg
     6001 cccccgcgct cacgccggcc gcgactccac gtcggcgggg aatggcggcg ccggctcgag
     6061 cgccacggcg agctcggcct ggtagtactg ctcgagctcg cgcaggaacg cgtgcaggtc
     6121 gcgccgcggc tcgaagcgcc ggccgaggcg cacgcggtac gacagcggca gcggcggccg
     6181 gcgcgtcagc ggccagccct tgccgaggaa cgggctgtcg gtctcgatga atacggtctg
     6241 caccggcacg ccggcctgct gcgcgatgag tgcgagcgcg ccacggaagg catcgacggg
     6301 cgcgcgcgtg gtgcgcgtgc cctcgggaaa cagcagcagc tggccgcctt cgcgcagctc
     6361 ggcgagggcg tggcgcacca tgccgatcgg cgaatcgttg cggatgtagc cggcgagccg
     6421 cgcgccggcg ccgaacacca ggctctcggc gagcggcgct ttcagcacgc aggccgcctg
     6481 cggcaggcgc gacagcagca gcaccgcgtc gagcagcgac gggtggttgg gggcgaggat
     6541 catcggcccg gcgccgcgta gcctgtccag ctccccgagc tcgaagcggc aagcgccaac
     6601 cgcctgcagc gcccagaggt agaagcggaa gccgcgcgcg atcacgcctc gcccgagcgc
     6661 gcgcccggcg cgcgcgggca gcagcaggcg cagcggcacc gcgagcaggc tccaggtgag
     6721 gctgccgagg ccgagcagcg cgaggccata ccacaacgcc gcgtaggccc acagcgcgcg
     6781 cagggagcgc aggatcacgc cgccctcact ccgccgcctc ggcgcgctcg cgcgatacgc
     6841 cgagcaccca ggcaatgcct gcccccgcgg cgacgtaggt gtcgcgcttg accagcggac
     6901 tcgcctcgaa cgctgcgccg ctgagcgtat cggcgcgcac gaacgccccc acccagaatc
     6961 cggcgaaacg gcggctggcc gcggcgagga actggaaccc ggcgtagccg cccggcgccg
     7021 cgtaggtcgg ccgcgccggc gtcgcaaact gcggcgccac gccatagaag tactcgtgct
     7081 gccggcggct gccgtatacc ggccccgcga gcaggccgaa gttccagccg gcgagcgtgc
     7141 cgcgccccgc caggtcgaga ttcaggttgg gtgtcgccac ccagccgatg tgctcgggcg
     7201 acgcttccag cgtgaacgcc gcgcgcaagg gcaggcgcag ctcgagcagg tcgcggccgt
     7261 cccgcgagcg ccacagcgcg agctccaggc tcgggccgag ctcgaacgag ggccgcaggt
     7321 ccggcatccc ggcgcgggcg tcgttgcgcg agctgttcac cggaagcgag gctgcgacgc
     7381 tgatgttgag ctccacgcga tccgtatcga acagctcgcc gcggatgccg cgccggtcgg
     7441 ccttgaggaa atccccgcgg tacaccacgt agggcaccgg cagcacgtag ccacgcgcct
     7501 ggtcggagcc ccggtaatcc ggcagcgcaa agccgccgac gcccacgccc agctcccaca
     7561 gcggcagctc ctcggccgcc gccggcgccg cggcaagcgc gcacaggagc cacagcgccc
     7621 gcttcatgcg cccacattgg cggcatcgcg caggctgcta cgccgccgcc gccaggcgcg
     7681 cagcgcggcc cgcgggtcgg cgagcgacag gaagtagtac agccccttga aggcgagcag
     7741 cgacggcccg atcggcgagc gcgaaaagac gtcgcccgcc agcagcgcga gcagcgcctc
     7801 ccgcatgcgc agcaggttgc gcggccccat cagcagcccg cgcatcgccg ggctggtcac
     7861 gcgatagatg aaccaggaga actcgcgcgg gccgcgccgt gtgcgccggt caaagcgcgc
     7921 cagcgcgcgc cgcgccttgc ccggccggcg caggcaggtc tcgatggcgt cggcgccgtc
     7981 caccgcgctg gtcatggcga gcatcacacc ggtggagaac accgggtcga tgaaggcgta
     8041 ggcgtcgccg agcagcaggt agcccgggcc gtgggtgcgg tcgcaggcgt acgagtagtt
     8101 gccggtggct tcggcggcgg tgacgcgctc ggcgccctcg aggcgcgcgg cgagcttcgg
     8161 gcacagcgcg atcgtgtcca tcaggaactc gttcaccggc ttggtgcgcg acttgaggta
     8221 atagggccag accacggcgc cgacgctggt gatgccgtcg gcgagcggga tgaaccagaa
     8281 ccagccgtgc tcgaaccaga agatcgtgat gtcaccctcg caggaacccg cgttgcggcg
     8341 cgccccgcgg aaatgcgcgt agatcgccgc gctgttgtgc cgggcgttgc ggcgcttggc
     8401 acccaggcgg ccgccgagaa acgtatctcg ccccgaggcg tcggcgacaa agcgcgcgcg
     8461 ccacgtctcc gcgccaccgt cctcgcgctc gatctgcagg cacgcaccgc cgccgggcgc
     8521 gaactcgacg ccgcgcacgc ggcagccttc ctgcgcgagc gcgcccttgc gggcggcgtt
     8581 gcggaacagg atctcgtcga actgcgagcg ccgcacctga taggcgtacg gcagggactt
     8641 gtcccaggca tcggcgaacg ccaggcgcgt cggcctggga tgcgccggcg acacgagctc
     8701 cgcaccccag t
//
LOCUS       DBNHKE010000094         4087 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1443647, whole genome shotgun sequence.
ACCESSION   DBNHKE010000094 DBNHKE010000000
VERSION     DBNHKE010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1443647"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..870)
                     /locus_tag="AC16O9_05880"
     CDS             complement(106..870)
                     /locus_tag="AC16O9_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516181.1"
                     /translation="MPPIPLLARSTLLEARRSGLPWLAFGLLAAALGLAAFLSQVAVT
                     EGRELQAALAAALLRAGAAFLVAAHVTASVVREANDKVLELALTLPLSRSQYYLGKLA
                     GYAAIGLAFAAVFGMPMLLWASPASVALWSASLAAELLLVCAASLFFAVTLASVVPAI
                     AATAGLYLLARAIAAIQLLARGPLAEQTWAQSIARGAVDAVALLLPRLDAATRSEWLL
                     YAPPSPAEYGAAIGALILYSGLLCAAGLFDFHRRNL"
     gene            920..2644
                     /locus_tag="AC16O9_05885"
     CDS             920..2644
                     /locus_tag="AC16O9_05885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6516182.1"
                     /translation="MQADTQSAAAGRLPRAAWAISRRHWRFVVIGMLALLHVVALRGV
                     ADDGARVLLLAHLGLLLLWQPLLRSERRVSLAQALFIAGFAAALVVFLNWWLLAVWSV
                     VLAGLVGGRVFLHQARWQRRFYLMVFAYLVALLVVVVLPQIAPRNEIDPAIRALAAWG
                     LPALFVLMALTPAESDPPEAAQVIDYFYSIFLMLMLGVVVLGSFAFMTLGQIGYLQSL
                     TYIVFVIAGGLLAIGLAWSPRTGYAGLNVFISRYLLSIGLPFEQWLQVLAELSHAEAR
                     PERFLEEAVVALARLPSVEGAAWRARGTEGEVGGRSAYAVEFEIREMSLTIYSRYRTS
                     PALHGHLRLLAQLLAEFYLAKLRERALQEASYMQAVHETGARVTHDVKNLLQSLNVLC
                     SVAAAESGDSPQALALMRRQLPAITQRLAATLDKLQRPVPEQDSRVPLAEWWQALARQ
                     YRDRGVEFAEGVAPGSTRIPRGLFDSVADNLLANALAKRAAQPGVRVRVALLPEGAEG
                     LRVCDTGRAVPEQIAGSLLLAPVQSDSGLGIGLYQAARQAAAAGYALRLESNRDGEVC
                     FALRRAQA"
     gene            complement(2641..3480)
                     /locus_tag="AC16O9_05890"
     CDS             complement(2641..3480)
                     /locus_tag="AC16O9_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR6516183.1"
                     /translation="MERPDHPRGLDRAEPDVPARLPSLMRARPARRARGFTLTELAVV
                     FTIISLLLAAAMYTLSAQTDQRDLEETRRRLEAARELLLAFALVNGRLPCPASTTTGA
                     TAGFEAPAGGGTCTNSYSGWLPARTIGFQVTDAAGNAIDAWGNAIRYAVSATAPTCPI
                     APTSPHFTNSTNLRANGIGCQPNDLVVCQSASGITATACGTANPVTNQNTLAAIVFST
                     GKNGATGAGGIDEGANLNGDRVFVWHTPAPASAANGAFDDQFNWISAGELYGKLIAAG
                     LLP"
     gene            complement(3408..>4087)
                     /locus_tag="AC16O9_05895"
     CDS             complement(3408..>4087)
                     /locus_tag="AC16O9_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516184.1"
                     /translation="RLRMGRTVVADHPILSATGRALRFSGGSSAINSGNTVTGATSGA
                     SAAVQVLINSGDWSTSNAQGVLFFSSVSGSFLSGETLQVSGSTKATAAGADFDIGWFV
                     RNQWYRNIFYAVAQNNTADVLPSVGGCGSGNCLRFNDPATRNIRALLVLAGSGLPTRL
                     PRPNNDRLAYVEDQNGDYGSLWEQRRPKTSRVAIAAPPLVAPWNDRIILVDWIAPNPT
                     FPLAYLP"
BASE COUNT          558 a         1397 c         1523 g          609 t
ORIGIN      
        1 caggcggcgg gcgcagcgct tgcacgccga tctgcgcggc gagcgccagc ccgagcactg
       61 cccatagcca gccgggcacc gcggccagcg ggcgctcgga tgcgctcaca ggttgcgccg
      121 gtggaaatcg aacagcccgg ccgcgcacag caggcccgag tacaggatca gcgcgccgat
      181 cgccgcgccg tactcggcgg gcgacggcgg cgcgtaaagc agccattcgc tgcgcgtggc
      241 ggcgtccagg cgcggcagca gcagcgcgac ggcgtccacc gcgccacgcg cgatgctctg
      301 cgcccaggtc tgctcggcca gcgggccgcg cgccagcaac tggatggcgg cgatggcgcg
      361 cgccagcagg tacaggccgg ccgtggcggc gatggccggt accacgcttg ccagggtcac
      421 cgcgaagaac aggctcgccg cgcacaccag gagcagctcc gcggcaagcg aggcactcca
      481 caacgcgacg ctcgccgggc tagcccacag cagcatcggc atgccgaaca cggcggcgaa
      541 cgcgaggccg atcgccgcgt agccggcgag cttgccgagg tagtactggc tgcgcgacag
      601 gggcagcgtg agcgcgagct cgagcacctt gtcgttggct tcgcgcacca cgctcgcggt
      661 aacgtgcgcg gcgacgagga aggcggcgcc ggcgcgcagc agtgccgcgg cgagcgccgc
      721 ctgcagctcg cggccctcgg tcaccgccac ctgcgacagg aacgccgcga gcccgagcgc
      781 cgcggcgagc agcccgaatg cgagccaggg caggccgctg cggcgtgcct cgagcagcgt
      841 gctgcgggcg agaagcggga tcgggggcat ctatcgcggg ccggggaatc tgcgtatctt
      901 tgcctgcgcc gccccgatca tgcaagccga cacacagagc gccgccgccg gccgcctgcc
      961 gcgggcggcg tgggccatca gccgacgcca ctggcgcttc gtggtgatcg ggatgctggc
     1021 gctgctgcac gtggtggcgc tgcgcggcgt cgccgacgac ggcgcgcgcg tgctgctgct
     1081 cgcgcacctg gggctgctgc tgctgtggca gccgctgctg cgcagcgagc gccgcgtctc
     1141 gctggcgcag gcgctgttca tcgccggctt cgcggcggcg ctggtggtgt tcctcaactg
     1201 gtggctgctc gccgtgtgga gcgtggtgct cgcggggctg gtcggcggca gggtgttcct
     1261 gcaccaggcg cgctggcagc ggcgcttcta cctcatggtg ttcgcctatc tcgtcgcgct
     1321 gctggtggtg gtcgtgctgc cgcagattgc gccgcgcaac gagatcgatc cggcgatccg
     1381 cgcgctcgcc gcctgggggc tgcccgcgct gttcgtgctg atggcgctga cgcccgccga
     1441 gagcgacccg cccgaggcgg cgcaggtgat cgattacttc tacagcatct tcctgatgct
     1501 gatgctcggt gtcgtggtgc tcggcagctt cgccttcatg acgctggggc agatcggcta
     1561 cctgcagtcg ctcacctaca tcgtgttcgt gatcgccggg gggctgctcg ccatcggcct
     1621 ggcgtggagc ccgcgcaccg gctacgccgg tctcaacgtc ttcatctccc gctatctgtt
     1681 gtccatcggg ctgccgttcg agcagtggct gcaggttctg gcggagctgt cgcacgccga
     1741 ggcccgcccc gagcgcttcc tggaggaggc ggtcgtggcg ctcgcgcggc tgccctcggt
     1801 cgagggtgcg gcgtggcgcg cgcgcgggac cgagggcgaa gtgggcgggc gctcggccta
     1861 cgccgtggag ttcgagattc gcgagatgtc gctcaccatc tactcgcgct atcgcaccag
     1921 ccccgcgctg cacggccacc tgcgcctgct ggcgcagctg ctcgcggagt tctacctcgc
     1981 caagctgcgc gagcgggcgc tgcaggaagc gagctacatg caggcggtgc acgagaccgg
     2041 cgcgcgggtg acgcacgacg taaagaacct gctgcaatcg ctgaacgtgc tgtgctcggt
     2101 ggcggctgcc gagagcggcg actcgccgca ggcgcttgcc ctcatgcgcc gccagctgcc
     2161 ggcgatcacg cagcgcctgg cggcgacgct ggacaagctg cagcggcccg tgccggagca
     2221 ggacagccgc gtgccgctcg ccgagtggtg gcaggcgctg gcgcgccagt accgggaccg
     2281 cggtgtcgag ttcgccgagg gcgtcgcgcc cgggagcacc cggattcccc ggggactgtt
     2341 cgacagcgtc gccgacaacc tgctcgccaa tgcgctcgcc aagcgcgcgg cgcagcccgg
     2401 ggtgcgcgtg cgcgtggcgc tgctgccgga gggcgctgaa ggcctgcggg tgtgcgacac
     2461 ggggcgcgcg gtgcccgagc agatagcggg ctcgctgctg ctcgcgcccg tgcagtcgga
     2521 cagcggattg ggcatcggcc tgtaccaggc cgcgcgccag gccgccgccg cgggctacgc
     2581 gctgcgcctg gaatcgaacc gcgacggcga ggtgtgcttc gcgcttcggc gcgcgcaggc
     2641 ctagggcagc aggccggcgg cgatcagctt gccgtagagc tcgccggcgc tgatccagtt
     2701 gaactggtcg tcgaaggcgc cgttcgcggc gctcgccggc gcgggcgtgt gccagacgaa
     2761 tacccgatcg ccattcaggt tcgccccttc gtcgatgccg cctgcgccgg tcgcgccgtt
     2821 cttgcccgta gaaaagacga tggccgcgag ggtgttctgg ttggtgaccg gattggccgt
     2881 gccgcaggcc gttgccgtga tgcccgacgc ggactggcac acgacgaggt cattcggctg
     2941 gcagccgatg ccgttcgcgc gcaaattggt gctgttggtg aagtgcggcg acgtcggcgc
     3001 gattgggcag gtcggggcag tggccgacac cgcataacgg atcgcattgc cccaggcgtc
     3061 gatggcgttg cccgccgcgt cggtgacctg gaagccgatc gtgcgtgccg gcagccagcc
     3121 gctgtaggaa ttcgtgcatg tgccgccgcc ggcgggcgcc tcgaatccgg cggtcgcgcc
     3181 cgtcgtcgtg ctggcggggc agggcagccg gccgttgacg agggcaaagg cgagcagcag
     3241 ctcgcgcgcg gcctccagcc gccgccgcgt ttcctccagg tcgcgctggt cggtctgcgc
     3301 cgagagcgtg tacatcgccg ccgccagcag caggctgatg atcgtaaaga cgaccgcaag
     3361 ctcggtgagc gtgaagccgc gcgcgcggcg agccgggcgg gcacgcatca gggaaggtag
     3421 gcgagcggga acgtcgggtt cggcgcgatc cagtccacga ggatgatccg gtcgttccac
     3481 ggcgcaacga gtggtggtgc cgcaatcgca accctgcttg ttttcggccg gcgctgctcc
     3541 cacagcgagc cgtagtcgcc gttctggtcc tcgacatagg cgagccgatc gttgttcggt
     3601 cgcgggagcc gcgtcggcag gccgctgccc gcaaggacca agagcgcacg gatgttgcgc
     3661 gtcgcggggt cgttgaagcg caggcaattg cccgagccgc agccgcccac ggacggcagc
     3721 acgtccgcgg tgttgttctg cgccaccgcg tagaagatgt tccggtacca ctggttgcgc
     3781 acgaaccagc cgatatcgaa gtcggcaccc gccgccgtcg ccttcgtgga gccgctgacc
     3841 tgaagggtct cgccgctcag gaatgatccg ctcacgctgg agaaaaagag cacgccctgc
     3901 gcgttgcttg tcgaccagtc gcccgagttg atcagcacct gaacggctgc cgacgcccca
     3961 gaggtcgcgc cggtcacggt attgccgctg ttgatcgcgc tggagccgcc gctgaagcgc
     4021 agggccctgc cggtcgcgct caaaatgggg tgatcggcca ccacggtgcg gcccatgcgc
     4081 aggcggt
//
LOCUS       DBNHKE010000095         6115 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_15708, whole genome shotgun sequence.
ACCESSION   DBNHKE010000095 DBNHKE010000000
VERSION     DBNHKE010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_15708"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="AC16O9_05900"
     CDS             <1..817
                     /locus_tag="AC16O9_05900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6516185.1"
                     /translation="HGKPPEGQLAQLRREVGDTLIERVLLLAEARRRGIEPEAEKTRA
                     ALAAFEARYGNDPRWQRDRDRLLPTLTQSLEEQNILERLEAAARVAPEPGEAELRAYY
                     QAHPDEFTEPERLRLSLILLKVDPAASQDDRDKAGEEARAIRRRLDEGADFAALARER
                     SGDMSAPNGGDMGYVHRGMLPEPVHARLDELAPGALSEPITLLEGFAILRLDERRPAQ
                     LRGFEESSQRAAELWRREREERQWRELKAALRRAATIEVLDRSRYPTAELVPK"
     gene            874..2907
                     /locus_tag="AC16O9_05905"
     CDS             874..2907
                     /locus_tag="AC16O9_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516186.1"
                     /translation="MSHAREQGRHELCALLVGGWVWVLSGIAAAADPEPGPVAQAPEL
                     AARTDVAPAAAPRRGEGESALIGVAPPQVPQEGAAPASEDAATMAQGVQSEAAEPPAA
                     PGTGLRAWGIAPIRWGGYLDLQYHSASAEGQPRRRQLVESLTFKGSSHIWQPWIAQVS
                     GDATLFAAQARVDNDGGETSSRNVVGGGQLALFPMSRFPFTATYRESDSRVSGELVTN
                     AYTSRRYGLTQSYTPPQGGSNYRLSYERSGVTSEALGTDTAKALNASMNWSGGFHTLN
                     VSGNTYTNTRDYSGDTSSLESTYVGYSYRPASTFAVENVASYSDTEYHLVGGGLPMDL
                     RSRYLQLSSFATWRPAETSPLRVSGGARLFENRTGSNGATVDAQTLTAHAAASYALSS
                     NANLRGGGSVTRTTRGSVSSVHSDQNVGADYYSDLIQLGEYSYGWNAGTTLGNQTGGE
                     SSRQTVSGQLGHRVTRNIALDVGSALGINAGQSYSAMRDSLAPTSQSLTHQGGVSWSR
                     RASQDTSTLVSLNVSDSRTRGYAEQHFQLVNLQGSGQMQFSRYSSANANMTLQATRQT
                     TYPAFATGATTPPTTYTSSSAGGSLSYRHARAFDVPRLSYYALLSLNQQQGRSRFEGD
                     INAPIEQVNWSLEQRLGYAIGRLESQLSLQVAETGGRTYGLLLFRVLRRFGAL"
     gene            2949..3380
                     /locus_tag="AC16O9_05910"
     CDS             2949..3380
                     /locus_tag="AC16O9_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR6516187.1"
                     /translation="MRQRLKYGCGGWAALGAMAAVLLLAGGSVGDVRAEYGDVVMNQY
                     SEAAGMRPAVFPHWFHRVRYSCKVCHSDLGIKLQAGGTGIDMLKIIDGAYCGACHNGN
                     VAWSVERCDLCHSGKPGTPTQVHQSTIQKLPAPAAASGAKK"
     gene            3390..4001
                     /locus_tag="AC16O9_05915"
     CDS             3390..4001
                     /locus_tag="AC16O9_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR6516188.1"
                     /translation="MAKTSWMALAASLLVLAGMPAAAQVAADSANAFNRLLKPAAQRN
                     LPPAEDGIHDPVNPGTRMLQAPLESFGPLPKRNSGNRVDWVGAQADGKIRPLWNAADH
                     KESAAAFDLNIVREVKGSMPDVVFPHKQHTEQLDCAICHPAIFVPQKGANQMSMAAII
                     LGQGCGACHGRVAFPIVECRLCHSRAKQAAGAGARAAGGQAGR"
     gene            3998..4951
                     /locus_tag="AC16O9_05920"
     CDS             3998..4951
                     /locus_tag="AC16O9_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516189.1"
                     /translation="MNAARADRWPGSALRSRLGPGLALAAGLLVLCPTALLGQPATPA
                     GQAGAGQQAPPDKAQLERRLVSVGTLIEKSSAARQIEASGDARALERRAAARDTYRQA
                     LQARDAGDDAAAARLASEASRLMFEAAKLAGRDRGREDQQRAAFDARLKSVQALLAAQ
                     QRISAEKPGTPHAAETGRSIEELIDEARRLAAANELQKAAAALDRAYLLAKAAVSSMR
                     AGDTLVRSLNFASPEEEYRYELDRNDTHMMLIKVFGADEARMAGADNMLKGFVTRAVE
                     LRSSAERAAAGGDHAAAIEQLERSTRELVRAIRGLGIFIPG"
     gene            4959..5531
                     /locus_tag="AC16O9_05925"
     CDS             4959..5531
                     /locus_tag="AC16O9_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR6516190.1"
                     /translation="MTDNFGLRKRALRTLGAGALLLVLAAGAPAQDKRSWKPLAADGV
                     HDPASPAVTQLQAPGDALSKLPPASGGNLVDWGAALQQGLIVPRAGVGEKSGADAEVL
                     ELDVLLDPNGSLPPVRFAHADHTRWLDCGNCHPKPFQMEKGASGISMLRILDGEQCGV
                     CHGAIAFPLTECNRCHNLPWGAASAPKGKP"
     gene            5531..5923
                     /locus_tag="AC16O9_05930"
     CDS             5531..5923
                     /locus_tag="AC16O9_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR6516191.1"
                     /translation="MSAHRAVLNLVALAAGLFLMVAHAPSQAEYGDLILNRQAEKNGM
                     RPVVFPHWFHRIRFRCGVCHGEMGFEMRAGATNATMADVSAGKFCGSCHNGTIAWGAD
                     RCDLCHSGLPGRATGVVGGDKTRGPGRW"
     gene            5934..>6115
                     /locus_tag="AC16O9_05935"
     CDS             5934..>6115
                     /locus_tag="AC16O9_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516192.1"
                     /translation="MPAVANAGGHGPGSRFAWARIAAIIWAVVLGGCATDTAPGAAMA
                     TGAAAPRLLTPWVSLSG"
BASE COUNT         1009 a         2094 c         2189 g          823 t
ORIGIN      
        1 ccacggcaag ccgcccgaag ggcagctcgc gcaactgcgc cgcgaggtcg gcgataccct
       61 gatcgagcgc gtgctgctgc tcgccgaagc gaggcggcgc ggcatcgagc cggaggcgga
      121 aaagacgcgc gcggcgctgg ccgcgttcga ggcgcgctac ggcaacgatc cacgctggca
      181 gcgggaccgc gatcgattgc tccccacgct gacacagagc ctcgaagagc agaacattct
      241 cgagcggctc gaggccgccg cgcgcgtggc gcccgagccc ggcgaggcgg agctgcgcgc
      301 gtattaccag gcccacccgg acgagttcac cgaaccggag cggctgcgcc tgtcgctgat
      361 cctgctgaag gtggatcccg cggcttcgca ggacgatcgc gacaaggcag gcgaggaagc
      421 gcgcgcgatc cggcggcgcc tcgacgaggg cgccgatttc gccgcgctgg cgcgcgagcg
      481 ctcgggcgac atgtcggcgc cgaacggcgg ggacatgggg tacgtgcacc gcggcatgct
      541 cccggagccc gtgcatgcgc ggctcgacga gctcgcgccc ggcgcgctgt ccgaaccgat
      601 cacgctgctc gaaggctttg ccatccttcg cctcgatgag cgccggcccg cgcagctcag
      661 gggcttcgag gaatcgagcc agcgcgcggc cgagctgtgg cggcgcgagc gcgaggaacg
      721 gcagtggcgc gagctcaagg cggcgctgcg ccgcgccgcg acgatcgagg tgctcgatcg
      781 gtcacgctac ccgaccgccg agctggtccc gaaatgagga acgcgagacc gcgcgaccct
      841 ccggaaaaac gggcgatcga caccgagcga cccgtgagcc acgcgcgaga acagggacgg
      901 catgaactgt gcgctctgct ggttggcggg tgggtgtggg ttttgtccgg catcgccgcg
      961 gccgccgatc ccgagccggg gccggtggcg caggcgccgg agctggcggc acgcaccgac
     1021 gtcgcgcccg ctgccgcgcc ccggcggggc gagggcgagt cggcattgat cggcgtggcg
     1081 cctccgcaag tgccgcaaga gggcgcggcg ccggcgtccg aggacgccgc gaccatggcg
     1141 cagggcgtgc agagcgaggc ggcggagccg ccggccgcgc cggggaccgg cctcagggca
     1201 tgggggatcg cgccgatccg atggggcgga tatttggacc ttcaatatca cagcgcgagc
     1261 gcggagggcc agccgcggcg ccggcagctg gtcgagtcgc tgaccttcaa gggcagcagc
     1321 cacatctggc agccctggat cgcgcaggtg agcggcgatg cgacgctgtt cgccgcccag
     1381 gcgcgagtgg acaacgacgg cggcgagacc tcctcccgca atgtggtcgg cggcggtcag
     1441 ctcgcgctgt ttcccatgag ccgctttccg tttaccgcca cctaccggga gagcgacagc
     1501 cgcgtcagcg gcgaactggt caccaacgcg tacaccagcc ggcgctacgg gctgacgcag
     1561 agctacaccc cgccgcaagg cggttcgaac taccggctca gctacgagcg cagcggcgtg
     1621 acctcggagg ccctcggcac cgatacggcc aaagcgctga acgcgagcat gaactggagc
     1681 ggcgggttcc atacgctgaa cgtctccggc aacacctaca ccaatacccg cgactacagc
     1741 ggcgacactt cgtccttgga gtcgacctac gtcggctaca gctaccggcc cgcctcgacc
     1801 ttcgcagtcg agaatgtcgc cagttacagc gacaccgagt accacctggt gggcggcggc
     1861 ctgcccatgg acctgcgctc gcgctacctg cagctcagct ccttcgccac gtggcgcccc
     1921 gccgagacct cgccgctgcg cgtgagcggt ggcgcgcgct tgttcgagaa ccgcaccggc
     1981 agcaacggcg ccaccgtcga cgcgcagacg ctcaccgccc acgccgcggc cagctacgcg
     2041 ctcagcagca acgccaatct gcggggcggc ggctccgtca cccggacgac gcgagggagc
     2101 gtgagcagcg tgcacagcga ccaaaacgtg ggcgccgact actactccga cctgatccag
     2161 ctcggcgaat attcctacgg ctggaacgcc ggcacgacgc ttggcaacca gaccgggggc
     2221 gagagcagcc ggcagaccgt cagcgggcag ctcgggcacc gcgtgacccg caacatcgcg
     2281 ctcgacgtgg gctcggcgct cggcatcaat gccggccaga gctattccgc catgcgcgac
     2341 tcgctcgcgc ccacgtcgca gtccctcacg catcaagggg gtgtgtcctg gagccgtaga
     2401 gcgagtcaag acacgagcac gctggtcagc ctgaatgtct ccgattcgcg caccaggggc
     2461 tacgcggagc agcatttcca gctcgtcaac ctgcagggga gcggccagat gcagttcagc
     2521 cgctattcgt ccgcgaacgc gaacatgacg ctgcaggcca cgcgccagac cacctaccct
     2581 gcgttcgcca ccggtgccac cacgcccccc accacgtaca cgagcagcag cgcaggcggc
     2641 agcctgagct acaggcatgc gcgcgcattc gacgtgccgc ggctgagcta ctacgccttg
     2701 ctgagcctga accagcagca gggccgctcc cgtttcgagg gcgatatcaa cgccccgatc
     2761 gaacaggtca actggtcgtt ggagcaacgc ctcggttacg ccatcggccg gctggagtcg
     2821 cagttgagcc tgcaggtcgc ggaaaccggg ggccggactt atggcctgct cttattcagg
     2881 gtattgcggc gcttcggggc gttgtgacgg gtacgcggtt caatcagttt tttgcacagg
     2941 aggcgcacat gcgacagcgg ttgaaatacg ggtgcggcgg atgggccgca cttggcgcga
     3001 tggccgcggt gctgctgctg gcagggggca gcgtcggcga cgtccgggcg gagtatggcg
     3061 atgtggtgat gaaccagtac tcggaggccg cgggcatgcg cccggcggtg ttcccccact
     3121 ggttccatcg cgtgcgctac agctgcaagg tctgccacag cgacctcggc atcaagctgc
     3181 aagcgggcgg caccgggatc gacatgctga agatcatcga cggcgcgtac tgcggtgcgt
     3241 gccacaacgg caacgtcgcc tggtcggtgg agcgctgcga cctgtgccat agcggcaagc
     3301 ccggcacgcc gacccaggtg caccagagca ccattcagaa actgcccgcg ccggccgcgg
     3361 cgagcggcgc gaagaaatag gaggcagcca tggcaaagac atcctggatg gcgttggcgg
     3421 cttcgcttct cgtgctggcg ggcatgccgg cggcggcgca agtggccgcc gactcggcca
     3481 acgcgttcaa ccgcctgctc aagccggccg cccagcgcaa cctgccgccc gccgaggacg
     3541 gcattcacga tcccgtaaat cccggcactc gcatgctgca ggcgcccctc gagtccttcg
     3601 gcccactgcc gaagcgcaat tccggcaacc gcgtagactg ggtgggcgcg caggcggacg
     3661 gaaagatccg gccgctgtgg aacgccgcgg accacaagga aagtgccgcc gcgttcgacc
     3721 tcaacatcgt gcgcgaagtg aagggctcga tgccggacgt ggtctttccg cacaagcagc
     3781 acacggagca actggactgc gccatctgcc accctgcgat ctttgtgccg cagaaagggg
     3841 ccaaccagat gagcatggcg gcgatcatcc tgggccaggg ctgcggcgcc tgtcacgggc
     3901 gcgtcgcgtt tccgatcgtg gagtgccgcc tgtgccattc ccgcgcgaag caggctgccg
     3961 gcgccggcgc gcgcgccgcg ggagggcagg ccgggcgatg aatgccgcgc gcgccgatcg
     4021 ctggcccggg tccgcgcttc gctcccggct cgggccaggc ctggcgctcg ccgccggcct
     4081 gctggtgctg tgcccgaccg cgcttctcgg gcaaccggca acgcccgcgg gtcaggccgg
     4141 cgcggggcaa caggccccgc cggacaaggc gcagctcgag cggcggctgg tctcggtggg
     4201 cacgttgatc gagaaatcct cggccgcgcg ccagatcgag gcgagcggcg acgcgcgggc
     4261 gctggaacgc cgcgccgccg cacgcgatac ttatcggcag gcattgcagg cccgcgacgc
     4321 gggggatgac gcagccgccg cgcgcctcgc ctcggaagcg agcaggctga tgttcgaggc
     4381 ggcgaagctc gcggggcgcg accgcggtcg cgaggaccag cagcgcgcgg cgttcgacgc
     4441 gcgcctgaaa agcgtgcagg ccctgcttgc tgcacagcag cgtatcagcg cggaaaagcc
     4501 cggcacgccg catgccgcgg agaccggccg tagcatcgaa gaactgatcg acgaggcgcg
     4561 ccggcttgcc gccgcgaacg agctgcaaaa ggccgcggcc gcgctcgacc gggcctacct
     4621 gctcgccaag gcggcggtga gctcgatgcg cgccggcgac acgctggtgc gctcgctgaa
     4681 tttcgccagc cctgaggagg aataccgcta cgagctcgac cgaaacgaca cgcacatgat
     4741 gttgatcaaa gttttcggcg cggacgaggc cagaatggcc ggagcggaca atatgctcaa
     4801 gggcttcgtc accagggcgg tcgaactgcg cagcagtgcg gaacgcgcgg cggcgggcgg
     4861 cgatcacgcc gccgcgatcg agcagctgga acgctcgacg cgcgagctgg tgcgcgcgat
     4921 ccgcggcctg ggcatcttca ttcccggata ggtggacgat gacggacaac ttcggcctac
     4981 gaaagcgcgc gctgcggacg ctgggtgccg gcgcgctgct gctggttctc gccgcgggcg
     5041 cgccggcaca ggacaagcgc agctggaaac cgctcgccgc ggacggcgtc cacgatccgg
     5101 caagcccggc ggtcacgcaa ctgcaggcgc cgggcgatgc gttgtcgaaa ttgccgcctg
     5161 caagcggcgg caacctggtg gattgggggg cggcgctgca gcaagggctg atcgtcccgc
     5221 gcgcgggtgt cggcgagaaa tcgggcgccg acgccgaggt cctcgaactc gatgtgctgc
     5281 tcgatccgaa cggcagcctg ccgcccgtgc gcttcgcgca cgcggatcac acgcggtggc
     5341 tcgattgcgg caactgccat ccgaagccgt tccagatgga aaagggtgcc agcggcatca
     5401 gcatgctgcg catcctcgac ggcgagcaat gcggcgtctg ccacggcgcg atcgcctttc
     5461 cgcttaccga gtgcaatcgc tgccacaacc tgccgtgggg cgcggcctcg gcgccaaagg
     5521 gcaagccctg atgagcgcgc atcgcgccgt gctgaacctg gtcgcgctcg cggcgggcct
     5581 gttcctgatg gtggcgcacg cgccgtcgca ggccgaatac ggcgacttga tcctgaacag
     5641 gcaggcggag aagaacggca tgcggccggt ggtgtttccg cactggttcc atcgcatccg
     5701 tttccggtgc ggagtctgcc acggcgagat ggggttcgag atgcgcgccg gcgctacgaa
     5761 cgcgaccatg gccgacgtca gcgccggaaa attctgcggc agttgccata acggcacgat
     5821 cgcctggggc gccgatcgct gcgacctgtg ccattccggt ctccccggcc gggccaccgg
     5881 tgtcgtgggc ggcgacaaga cgcgtggccc cgggcgctgg taggtccgcg cgcatgccag
     5941 cggtcgcgaa cgcgggcggg catgggcccg gatcgcgctt cgcatgggcg cggatcgcgg
     6001 caatcatctg ggcggtggtg ctcggcggct gcgcgaccga taccgcgccg ggcgcggcca
     6061 tggcgacggg cgccgcggcg ccgcgactgc tgaccccgtg ggtgtcgctc tccgg
//
LOCUS       DBNHKE010000096         4303 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_809126, whole genome shotgun sequence.
ACCESSION   DBNHKE010000096 DBNHKE010000000
VERSION     DBNHKE010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_809126"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="AC16O9_05940"
     CDS             complement(<1..277)
                     /locus_tag="AC16O9_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516193.1"
                     /translation="MRRFALALWFVLALASLPVGAEQPDVLQRLFAEGMQHLQSGEAA
                     QAERVFRQMLEHTDSPRVKLELARALYAQGKFKEAKALFREVSRQSDT"
     gene            complement(285..1367)
                     /locus_tag="AC16O9_05945"
     CDS             complement(285..1367)
                     /locus_tag="AC16O9_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013466.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HIR6516194.1"
                     /translation="MKTLRLMVLVTAAPMIMSGCASGGGGDEASQDGWGASGAEFAQG
                     LKGFLSGLAGAATGQCPTCGSGSQASASTQPQSTASSAPVFADGVDVSVRVSKQMGVA
                     QDVGQPSAIGLQAESTYSETRSIRVFTASVWPSASGIGYFVPNGGTGFAAGRGIETLW
                     RTTTGWNESPSSTMFTSTPGTVALVANPYASGWNYQSFGVWASRQDDGTDRANGMSFG
                     SATTATPGGAVPNAGSATFYGKLAGLYVSRGGVGSVAAADIRVQANFSQRSLGFSSSS
                     TVTTRTPSTTAQVTTAPHLNLSGTLSYAPGSGAFSGAVATADGAMTGTSNGQFYGPAA
                     QELGGVFALKSATTVETFVGAYGAKR"
     gene            complement(1396..2592)
                     /locus_tag="AC16O9_05950"
     CDS             complement(1396..2592)
                     /locus_tag="AC16O9_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013466.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HIR6516195.1"
                     /translation="MQTMKRLVVALVAAVVVSGCASGGGGSTATATYADGSTRELTLP
                     ELLGCLLTAALLCPRSASQSTTAAAGSTQGASSTSGSGSRSSGTSGSTRAEPIAPSSQ
                     GSTTSSSPVPFTRWEDHRGGGAIARALGATASIRRAADVGVGEAITGSGGTDGGEIAV
                     LYDEASALSELRMYDRSLFPRAVAANPVGHPEIDMAWSASKATQTVFADVPSEISALI
                     ANPYALGWNYQSFGVWNRPDLSERTLEQVFAVSYGAATPGTAVPTTGSATFTGKLAGL
                     LVQPSAADYMVAANVRLQVDFGERSVGLASSGTSIMRDGISASSAPGLDLSGTMRYAP
                     GSSNSAGANPFSNSFSGTLTTADGSMSGTSNGRFYGPAAEEAGGAFLVQSPTGAAFTG
                     AYGAKR"
     gene            complement(2607..2840)
                     /locus_tag="AC16O9_05955"
     CDS             complement(2607..2840)
                     /locus_tag="AC16O9_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6516196.1"
                     /translation="MNGHINAGLQVAFLTPRETQVLQHISRGCTYEQVADRLGVSLHT
                     VCSHVKNTYRKLGVRSGAAAVMRAVELKLFGEV"
     gene            2991..>4303
                     /locus_tag="AC16O9_05960"
     CDS             2991..>4303
                     /locus_tag="AC16O9_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6516197.1"
                     /translation="MAQPRLTAADRPFFAALADVVYGNVFSANRAEVIARLLPGVRPE
                     ENKDPEALARVVQPRLEPFVQAGGLQAPGLSDADRKLLEPACLYVCYIRAIPNLDTLI
                     ERQAAKGGEPLLVAFADEVIGDLMRCGFDEERALRYLALYFQLRRGFYFISRGLAGKC
                     LSMRRLREALWNNIFTHDMRGFEAALWSRMEDFSTLLLGETGTGKGAVASAIGRSGFI
                     PFDRARRRFAANFTETFIAINLSQYPESLIESELFGHRKGAFTGAIDNHEGVFERCNE
                     HGALFLDEIGEVSIPVQIKLLQVLQERTFTAVGGREKKRFGGRVIAATNQPLGALRRE
                     GRFRQDFFYRLCSDVIEVPALRQRIAESEDELELLVRLLVARIAGAEVPELVTLVLDA
                     LDVNLPRGYPWPGNVRELEQAVRRILLTGRYRGEVAPGPGGGEEAL"
BASE COUNT          686 a         1480 c         1402 g          735 t
ORIGIN      
        1 gggtgtcgga ctgccgcgag acttcccgga acagggcctt cgcttccttg aacttgccct
       61 gggcgtagag cgcgcgcgcg agctcgagct tcacgcgcgg gctgtcggtg tgctcgagca
      121 tctgccggaa gacgcgctcg gcctgcgcgg cctcgcccga ctgcaggtgc tgcatgcctt
      181 cggcgaacag gcgctgcagg acgtccggtt gctctgcgcc cacagggagc gaggcaagcg
      241 ccaggacaaa ccacagggcg agcgcgaagc ggcgcatggc ggcgtcagcg cttggcgccg
      301 taggcgccga cgaacgtttc gaccgtcgtc gcggacttga gcgcgaagac gccgccgagc
      361 tcctgcgcgg ccgggccgta gaactgcccg ttcgacgtgc cggtcatcgc accgtcggca
      421 gtcgctactg ctccggagaa ggcgccagac cccggggcat aactcagagt tccgctcaga
      481 ttcagatgag gcgccgtcgt cacctgggcg gtggttgaag gagtacgggt cgtgacggtg
      541 ctgctcgatg agaagccgag gctgcgctgg ctgaagttgg cctggacgcg aatatccgcc
      601 gcagccaccg acccgacgcc gcctctgctc acatagagcc ccgcgagctt tccgtagaaa
      661 gttgcggagc ccgcgttcgg gacggcgccg cccggagtcg cggtggttgc gctgccgaac
      721 gacatgccat tggcacggtc tgtgccgtcg tcctgccgcg acgcccagac cccgaaggac
      781 tggtagttcc agccagatgc gtacggattg gcgacgagcg cgaccgtccc gggcgtgctc
      841 gtgaacatgg tgctggacgg gctttcgttc cacccggtcg tcgttcgcca gagcgtctcg
      901 atgccacggc ccgccgcaaa tccggtgccg ccattgggca cgaaataccc tatgccggaa
      961 gcggacggcc agacacttgc cgtgaacacg cggatgcttc gggtctccga gtaggtcgat
     1021 tccgcttgca gcccaatggc cgacggctgt ccgacgtcct gcgctacgcc catttgcttg
     1081 gatacccgaa cgcttacgtc cacgccgtcg gcaaacacgg gggcgctcga cgccgtgctt
     1141 tgcggttgag tggacgcgct tgcttgagat cccgatccgc aggtcgggca ttgtccggtc
     1201 gctgcccctg cgagcccgga taagaagcct ttcagccctt gggcgaattc cgcaccactg
     1261 gcaccccagc cgtcctgcga cgcctcgtcg ccacctccac cgctggcgca cccgctcatg
     1321 atcatgggcg cggcggtgac cagcaccatc aaacgaagag ttttcatgat tgttcctcgc
     1381 tttcgcgtgt cggcttcagc gcttggcgcc gtaggcgccc gtaaacgcgg caccagtcgg
     1441 cgattgcacc aggaaagcgc cgcccgcctc ttccgcagcc ggaccataga agcgcccgtt
     1501 cgacgtcccg ctcatgctgc cgtcggccgt cgtcagcgtg cccgagaacg agttcgagaa
     1561 cgggttggcg cccgccgaat tgctggagcc cggcgcgtac ctcatcgtcc cggagagatc
     1621 taggcctggc gcggaactcg cgctgatccc atcgcgcatg atgctcgttc cactcgaagc
     1681 gagacctacc gagcgctcgc cgaagtcgac ctgcaggcgt acgttcgctg cgaccatgta
     1741 gtccgccgcg ctcggctgga caagcaatcc tgcgagcttt ccggtgaagg tggcgctgcc
     1801 tgtcgtgggc acggcggttc ccggagtggc cgcgccatag gaaactgcga agacttgttc
     1861 cagcgtccgc tcgctcaggt ccggacggtt ccagacgccg aacgactgat aattccagcc
     1921 aagtgcatat gggttggcga tcagcgcgga gatctcggag gggacgtcag cgaatacggt
     1981 ctgtgtcgcc ttcgacgcgg accaggccat gtcgatttcg ggatggccga ccggattcgc
     2041 ggcgaccgcg cgcggaaaca gcgagcggtc gtacatcctg agctcgctca gcgcgctagc
     2101 ttcatcgtag agcacggcga tctcgccgcc gtcggtgccg cccgaacccg taatcgcttc
     2161 ccccacgcct acgtcagcgg cacgacggat tgatgccgta gctccgagcg cgcgcgcaat
     2221 ggcaccgccg ccgcgatgat cctcccaacg ggtaaacgga accggcgacg aggaagtcgt
     2281 gcttccctgc gagctcggcg cgatcggctc ggcgcgggtc gatccgcttg ttccgctcga
     2341 ccgggatcca ctgccggacg tcgaagaggc gccttgcgtg gacccggctg cagccgtcgt
     2401 cgactggctt gccgacctcg ggcatagcag cgccgcggtc aacagacaac cgaggagttc
     2461 aggcagcgtc agctcccgcg tcgagccgtc cgcgtatgtc gcggttgccg tgctgccgcc
     2521 cccaccgctc gcgcagccgc tcaccacaac cgcagccacc agcgcaacaa ccagccgttt
     2581 catggtttgc atgaccatca ctcctttcaa acttccccga acaacttcag ctccaccgcg
     2641 cgcatcaccg ccgcggcccc cgagcgcacg ccgagctttc gatacgtgtt cttgacgtgc
     2701 gagcagaccg tgtgcagcga cacgccgagc cggtcggcga cctgctcgta ggtgcagccg
     2761 cgcgagatgt gctggaggac ctgcgtctca cgtggcgtga ggaaggcgac ttgcagccca
     2821 gcattgatgt gtccgttcat ggctcgctcc ttagtgggag tgcaccctgg tatgagcagc
     2881 ccccgtgcca gggacataac ctcttgttta attgaacttc tctgttttcc gcatcgatcc
     2941 cggagccact gcgtctggca tttactgacg cacaggtcta catttcctgc atggcacagc
     3001 cgcgcctcac cgccgcggac cggccgttct tcgcggcgct cgccgacgtc gtctacggca
     3061 acgtcttctc cgccaaccgc gccgaggtca tcgcccgcct gctgcccggc gtgcggcccg
     3121 aggagaacaa ggacccggag gcgctcgcgc gcgtggtgca gccgcgtctc gagccgttcg
     3181 tgcaggccgg cgggctgcaa gcgcccgggc tgagcgacgc cgaccggaaa ctgctcgagc
     3241 ccgcatgcct ctacgtctgc tacatccgcg ccatcccgaa cctcgacacg ctgatcgagc
     3301 gccaggcggc gaagggtggc gagccgctgc ttgtggcttt cgccgacgag gtgatcggcg
     3361 acctgatgcg ctgcggcttc gacgaggaac gcgcgctgcg ctacctggcg ctgtacttcc
     3421 agctgcgccg cggcttctac ttcatcagcc gtgggctcgc gggcaagtgc ctgtcgatgc
     3481 gccgcctgcg cgaagcgctg tggaacaaca tcttcacaca cgacatgcgc ggcttcgagg
     3541 cggcgctgtg gagccgcatg gaggatttct cgaccctgct gctcggcgag accggcacgg
     3601 gcaagggcgc ggtggcctcg gccatcggcc gctccggctt catccccttc gaccgcgcgc
     3661 ggcgccgctt cgccgccaac ttcaccgaga ccttcatcgc catcaacctc tcgcagtacc
     3721 ccgagtcgct gatcgaatcg gagctcttcg gccatcgcaa gggcgccttt acgggcgcca
     3781 tcgacaatca cgagggcgtg ttcgagcgct gcaacgagca cggggcgctg tttctcgacg
     3841 agatcggcga ggtctcaatc ccggtgcaga tcaagctgct gcaggtactg caggagcgca
     3901 cgttcacagc agtgggcggg cgcgagaaga agcgcttcgg gggccgcgtc atcgcggcca
     3961 ccaaccagcc gctcggcgcg ctgcgccgcg aagggcgctt ccgccaggat ttcttctacc
     4021 gcctgtgctc cgacgtgatc gaggtgccgg cgctgcgcca gcgcatcgcc gaatcggaag
     4081 acgagctcga gttgctggtg cgcctgctgg tggcgcgcat tgccggcgcc gaagttcccg
     4141 agctcgtgac cctggtgctg gacgcgctcg acgtcaacct gccgcgcggc tatccctggc
     4201 ccgggaacgt gcgcgagctc gagcaggcgg tgcgcaggat cctgctcacc gggcgctacc
     4261 ggggcgaggt ggcaccgggc cctggcggcg gcgaagaggc act
//
LOCUS       DBNHKE010000097        17926 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1285389, whole genome shotgun sequence.
ACCESSION   DBNHKE010000097 DBNHKE010000000
VERSION     DBNHKE010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1285389"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16O9_05965"
     CDS             complement(<1..184)
                     /locus_tag="AC16O9_05965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6516198.1"
                     /translation="MSAGKGMGEGGAAAMKDCDVAVIGGGPAGSTVAALLAERGFRVA
                     LFEKSRHPRFHIGESLL"
     gene            complement(206..2071)
                     /locus_tag="AC16O9_05970"
     CDS             complement(206..2071)
                     /locus_tag="AC16O9_05970"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR6516199.1"
                     /translation="MFELIQKRKRLAQVVLVLLVIPFAFFGLESYTRSVGGRDDVATV
                     NGSPITQAEFAEELRRQQDRLRAAFGPNIDAEALDTTESRLALVDSLVSQRAVADAAL
                     RGGLSVSDEQLRETIAAVPAFQGENGFSRANYEALLRAQNMTPQTFEARLRHDMALNQ
                     LTESVRSTAIASRAVTERLEAIQAERREVQEALVPAQPFLAEVKVSDAQIKAYYESNP
                     AEFSVPERVRAEYLVLSAEALGRRDAVSEADLKAAYEARASQYRVDEQRRASHILVQS
                     EAEATKLAAEVRKNPGRFAELAKKHSQDPGSAADGGDLGFFGRGMMVPAFEEAAFRLR
                     EGEISAAVKSDFGWHLIRLTGIQPAKVRPLAEVRDELAAELSRQLGARRFAEAAETFS
                     NMVYEQSDSLKPVAERFKLPLQTSGWISRAVPAPEAGVLASPKLQGALFADDAVKARR
                     NTDAIEAAPNVLVSARVIEHQPASQKKLEDVRADIERRLRSQDAARLAQQAGESKLAA
                     LRKDGKSEVSWGAAKAVSRRSPQEVPAGALREIMAADPAKLPAYAGAARGTEGYMLYR
                     VVKVLEPEPKTEAQRTAERARAAQLAGARQLDAFVESLRARADIEIRQRNLEKKP"
     gene            complement(2097..2173)
                     /locus_tag="AC16O9_05975"
     tRNA            complement(2097..2173)
                     /locus_tag="AC16O9_05975"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2137..2139),aa:Asp,seq:gtc)
     gene            complement(2184..2259)
                     /locus_tag="AC16O9_05980"
     tRNA            complement(2184..2259)
                     /locus_tag="AC16O9_05980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2224..2226),aa:Val,seq:tac)
     gene            complement(2273..2545)
                     /locus_tag="AC16O9_05985"
     CDS             complement(2273..2545)
                     /locus_tag="AC16O9_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6516200.1"
                     /translation="MNKADLIEHIALQAEISKLAAERAVDALVGAVKSNLRKGQAVTL
                     AGFGTFYPGERQARTGRNPRTGAPLQIAAARVPRFRAGKALKDAVN"
     gene            complement(2555..4984)
                     /gene="lon"
                     /locus_tag="AC16O9_05990"
     CDS             complement(2555..4984)
                     /gene="lon"
                     /locus_tag="AC16O9_05990"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6516201.1"
                     /translation="MPEMNESPSGLTQYPLLPLRDVVVFPHMVIPLFVGRPKSIKAME
                     TAMEAGKSILLVAQKSAAKDDPGPEDMYEIGCVSGILQMLKLPDGTVKVLVEGASRAR
                     IRSATDLKSHWIAEAEPIAIEGAAPAEIEAMRRALLSAFDQYVKLNKKIPPEILTSLS
                     GIDEAGRLADTVAAHLPLKLEQKQQVLEMFDVKSRLEHLLSQLETEIDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGEGEEGADLEEMEKRIKRAHMHKDARRKAEAE
                     MKKLKLMSPMSAEATVVRNYIETLVNLPWKKRSKISRDIKAAQEILDKDHYGLEKVKE
                     RIVEYLAVQTRVDKMKAPILCLVGAPGVGKTSLGQSVARATNRKFVRMSLGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMTKVGVRNPLFLLDEVDKLGQDWRGDPSSALLEVLDPEQ
                     NHTFTDHYIEVEYDLSEVMFVATANTLNIPAPLLDRMEVIRLSGYTEDEKVNIAVRYL
                     LPKQMQSTGLKEGELSVSEAAIRDIVRYYTREAGVRALEREVSKICRKAVKQLLTDKQ
                     SGEGKARRITVTPKSLDKFLGVRKYSFGMAEKQNQVGQVTGLAWTEVGGELLTIETVT
                     MPGKGKTITTGKLGEVMQESIQAALSVVRNRSRKLGVQEDFYQKNDIHIHLPEGATPK
                     DGPSAGIGIATAMVSVLSGIPVRADVAMTGEITLRGEVLQIGGLKEKLLAAPRGGIKT
                     VLIPDENVKDLVDIPDNVKNRLEIIPVKWIDKVLEVALERQPEPLPAPEAAAPLPSAD
                     GKAGTATAVKH"
     gene            complement(5073..6344)
                     /gene="clpX"
                     /locus_tag="AC16O9_05995"
     CDS             complement(5073..6344)
                     /gene="clpX"
                     /locus_tag="AC16O9_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR6516202.1"
                     /translation="MSDKPAAGDKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCN
                     DIIREETASDKGGKGARSDLPTPHEIRNILDQYVIGQDQAKKILSVAVYNHYKRLKHL
                     SKNDEVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVEN
                     IIQKLLQNCDYDVDKAKRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     IASVPPQGGRKHPNQDFVQVDTTNILFICGGAFDGLEKIVRNRTEKTGIGFGAEVRSP
                     GNSKATFDLLKAVEPEDLIKYGLIPEFVGRLPVVAALEELDEHALIQILTEPKNALIK
                     QYQRLFLMEGVELEVRTAALGAVSRRALARKTGARGLRSILENVLLETMYELPTLENV
                     SKVVIDEQMISADGKPLLIYSDQQKVAGSSA"
     gene            complement(6345..6971)
                     /gene="clpP"
                     /locus_tag="AC16O9_06000"
     CDS             complement(6345..6971)
                     /gene="clpP"
                     /locus_tag="AC16O9_06000"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR6516203.1"
                     /translation="MNWHNRMPEANDLGLIPMVIEQSGRGERAYDIYSRLLKERVVFL
                     VGVVTESTANLIVAQLLFLESENPDKDIFFYINSPGGSVSAGLAIYDTMQFIKPDVST
                     LCVGQAASMGSLLLAAGDKEKRFVLPNSRVMIHQPMGGFQGQATDIEIHAKEILYLRG
                     RLNEILAKHTGKPVETIARDTERDFFLGAEDAVKYGLVDKVLTTREPA"
     gene            complement(6968..8272)
                     /gene="tig"
                     /locus_tag="AC16O9_06005"
     CDS             complement(6968..8272)
                     /gene="tig"
                     /locus_tag="AC16O9_06005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR6516204.1"
                     /translation="MAANVEQLGALERRLQMKVPLSGIEQEIDQRLKKLARNAKMPGF
                     RPGKVPMKVLAQTYGPQVRSEVLGDAVQKAFSDAVREANLRVAGQPRIEPKKDAAADA
                     LEFSATFEVYPEFRIGDVAAATVERPQVSVDDAAVDQTLEILRKQRVTYAEAARAAQS
                     GDRVIVDFAGTIGGEPFPGGKADGFAFVLGEGRMLPEFEAAAGGAAPGEAKNFSVTFP
                     PDYHGKEVAGKTAEFALTVKKVEEPRLPALDADFAKALGVADGDLAKMRAEVRQNVER
                     ETAKRIEARVKQQAMQALLEATPLELPKSLVAMETRELVERAAADLRARGLKFEKLPL
                     DPQAFEASAKRRVALGLIIAELARAEKLQPKPAEVRALIEQEAQSYESPAEVVKWFYM
                     QPQRLSEMEGLALEANVVQWVLQKAKVVDKAMPFDELMGSNA"
     gene            complement(8302..8386)
                     /locus_tag="AC16O9_06010"
     tRNA            complement(8302..8386)
                     /locus_tag="AC16O9_06010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8350..8352),aa:Leu,seq:tag)
     gene            8425..9210
                     /gene="hpnC"
                     /locus_tag="AC16O9_06015"
     CDS             8425..9210
                     /gene="hpnC"
                     /locus_tag="AC16O9_06015"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - geranylgeranyl diphosphate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HIR6516205.1"
                     /translation="MSVAHYENFPVASLLLPAPLRAPVAVIYRFARGADDIADEGDDA
                     PQERLRKLEEFRRQLGAPAAPLFEDVARIVAEHALPRQLFGDLLDAFAQDVTKTRYAD
                     YAELRDYCRRSANPVGRLLLHLFKRTSATDLQRSDAVCTALQLINFWQDAALDYAKGR
                     IYLPQDEMARHGVTERHLVEARCDDAWRALMAFQVQRTRALMLSGAPLGRALPGRVGL
                     EIRATVQGGLRILEKLERAGYDVFRRRPVLRGFDWLLVAARAL"
     gene            9207..10040
                     /gene="hpnD"
                     /locus_tag="AC16O9_06020"
     CDS             9207..10040
                     /gene="hpnD"
                     /locus_tag="AC16O9_06020"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046905 - 15-cis-phytoene synthase
                     activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HIR6516206.1"
                     /translation="MTPDAYCQQKAAASGSSFYYAFLFLPPARRRAITALYAFCREVD
                     DVVDETSDAQLAATKLAWWRQEVGLLFAGTPQHPVSKALAPAIAAYGITAQRLNEIID
                     GMQMDLTQTRYLDFAALERYCYHVAGVVGMLAAGIFGYSDPRTLDYARYLGTAFQLTN
                     IIRDVGEDARKNRIYLPIADLQQFGVAAADLLQARHSDAFVSLMRFQAERARGFYREA
                     LAALPRADRRAQRPGLIMAAIYAALLDEIERDGFQVLRARTSLTPLRKFWIAWKTWLA
                     V"
     gene            10025..11254
                     /gene="hpnE"
                     /locus_tag="AC16O9_06025"
     CDS             10025..11254
                     /gene="hpnE"
                     /locus_tag="AC16O9_06025"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HIR6516207.1"
                     /translation="MARRIAVVGAGYAGLAAAVTLAKRGAAVTVFESGPVPGGRARRV
                     ESGGEALDNGQHILIGAYDTLFGLMRTVGVPSDAVLRMPLELRYADGVSLRRLFLPAP
                     LGLLGGLLLARRLPWREKLGAVRFMAALRRINFRLAEDCSVSALLAQHRQDGRIGHYV
                     WQPLCVSALNTPPAQASANVFLAVLRDTLAGQAEASDLILPRIDLSRLFPEPAMAWLQ
                     QRGAEVRCGTTVRDLDPLRAEHAAVIVAVGPHQLKTLLPRSAPEFQYQPITTVYLQYG
                     PKTRLPLPMLGLAGGLVQWVFDRGALTGEKGRLAAVISAQGEHQQLTQDELARACHRE
                     LAAALKNLPEPKWSRVIAEKRATIAVAPGLRRPGIETPLPGVFLAGDYTDPEYPPTLE
                     AAARSGVRAAERALVDA"
     gene            11265..12299
                     /locus_tag="AC16O9_06030"
     CDS             11265..12299
                     /locus_tag="AC16O9_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6516208.1"
                     /translation="MEYRQLGASGFMVPALTFGTGTFGGGGEFFKAWGATDVAEAKRL
                     IDICLEAGVTMFDSADVYSGGLAEEILGKAVQGRRGELLISTKGSFRHGPGPNQVGSS
                     RQHLIAAVEASLTRLGTDHIDLYQLHGFDAMTPLEEVMQTLDGLVRAGKIRYIGCSNF
                     SGWHLMKSLAVSARYGLARHVAHQAYYSLVGRDYEWELMPLALDQGIGTVVWSPLAWG
                     RLGGKFRRGQPLPKDTRLASPATAQRGPQVPDEYLYRVVDALDAVAAECGKSVPQVAL
                     NWLLQRPSVTSIVIGARNEAQLRDNLGAVGWKLTPAQIAKLDAASAVPLPYPYWHQRA
                     IYGERNPPPV"
     gene            12296..12838
                     /locus_tag="AC16O9_06035"
     CDS             12296..12838
                     /locus_tag="AC16O9_06035"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR6516209.1"
                     /translation="MTRFEALHADITTLEVDAIVNAANEGLLGGGGVDGAIHRAAGPQ
                     LLEACRALAGCATGEAKITLGYRLKARHVIHTVGPVWRGGNRSEPALLASCYRRSLEL
                     AAQHALESIAFPAISTGVYGYPKEDAARVAVATVATHATGQLRRVIFCCFSAQDEALY
                     AALLRDPAAAAPPGPPARPR"
     gene            complement(12786..14132)
                     /gene="radA"
                     /locus_tag="AC16O9_06040"
     CDS             complement(12786..14132)
                     /gene="radA"
                     /locus_tag="AC16O9_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6516210.1"
                     /translation="MAKAKSVFVCAECGATALKWMGLCPSCGEAGTMSEQAAERGGRA
                     SGPAAHPVPLAEVEAREVERVGTGLPELDRVLGGGLVAGQVTLIGGDPGIGKSTLLLQ
                     ALAALGAKRKVLYVSGEESAEQVALRARRLGVGAAGVGLLAEIQLERVLGALQAARPE
                     VAVVDSIQTLWSDTLQSAPGSVAQVRECAAQLTRHAKKAGAALFIVGHVTKEGAIAGP
                     RVLEHIVDTVLYFEGDPHSAFRLVRAVKNRFGAVNELGVFAMTDSGLRAVANPSALFL
                     QRHEKDVAGSCVLATLEGTRPLLVEIQALVDAAHAPNPRRLTVGLEQNRLAMLLAVLH
                     RHAGIATMDQDVFVNAVGGVRIGEPAADLAAALAIVSSLTDRPIPGKLLAFGEVGLAG
                     EVRPAPRGQDRLKEAAKLGFERAVVPRANAPKGRIAGLEVIAVERVDQAVRLLRDL"
     gene            complement(14132..15202)
                     /gene="alr"
                     /locus_tag="AC16O9_06045"
     CDS             complement(14132..15202)
                     /gene="alr"
                     /locus_tag="AC16O9_06045"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6516211.1"
                     /translation="MPRPIRAVVKASALRHNLQVAREAAGEAKIWAVVKANAYGHGLE
                     RAARALEGADGFALLDFQEAVRLRLAGVTKPILMLEGFFRPLDVNLLFKYRLTPVIHN
                     PDQVELLKHMDFPAELDVYLKVNSGMNRLGFGIDEVRVMYNTLRLHPNIGRITLMTHF
                     ADAEGESGVQGQLRWFEELVRGIEAPRSFANSAALLRFREAREGWVRPGLMLYGCSPF
                     ADWSAAEIGLEPAMTLTSELIAVQNLAAGERVGYGFTFEAKEPMTLGVVACGYADGYP
                     RHAPTGTPVLVKGRRTRLVGRVSMDMMCVDITGIDEAYIGTPVTLWGEGLPADEVAAA
                     AGTVSYELLCALAPRVPVREEA"
     gene            15252..16529
                     /gene="lplT"
                     /locus_tag="AC16O9_06050"
     CDS             15252..16529
                     /gene="lplT"
                     /locus_tag="AC16O9_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HIR6516212.1"
                     /translation="MSLGFYIIMAAQFFSSLADNALLVAAIALLMLGDSPTWLTPYLK
                     FFFVISYVALAPYVGAFADRLPKGHVMFVSNSIKIVGCALMLFEVHPLAAYALVGLGA
                     AAYSPAKYGILTEYLPPTKLVIANGWIEGLTVGSIILGTVLGGVLISARVSGHLLGYD
                     LPLVDLGIDNAAEAAISVIVFVYATAALFNLYVPRTGVALKPLPPDPLATLRDFYHCC
                     QRLWTDRLGQISLAVTTLFWGAGATLQFIVIEWARVALDYNLSRAAILQGVVALGIAA
                     GAVVASLRVSLDRAVGVIPLGIGMGLVVIVMNFVSSIGIAIPLLILMGALAGYFVVPM
                     NALLQHRGHNLMGAGRSIAVQNFNENASILIMIAVYSALLGAGLSIYVVIVIFGLFVA
                     GTMTLVLLWYRRNRRVHREEIEALLEIARSEKH"
     gene            complement(16648..17337)
                     /locus_tag="AC16O9_06055"
     CDS             complement(16648..17337)
                     /locus_tag="AC16O9_06055"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6516213.1"
                     /translation="MSRRLEILEEMGLGPVWRLRQEKNPPVSAATTTAETGGSREEQV
                     ARMQWPELKAAVHNCTACGLRAGCKQTVFGVGDENANWLLVGEAPGAEEDRLGEPFVG
                     QAGKLLDNMLAALGLARDNNVYIANVLKCRPPGNRNPEPPEVATCSPYLLRQIDLIRP
                     KLIVAMGRFAVQTLLNTDATIGSLRGKVHQYHGVPLVVTYHPAYLLRNLPDKAKAWAD
                     LVFARRTIASS"
     gene            complement(17334..17795)
                     /gene="rimI"
                     /locus_tag="AC16O9_06060"
     CDS             complement(17334..17795)
                     /gene="rimI"
                     /locus_tag="AC16O9_06060"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR6516214.1"
                     /translation="MSAVLKDLPRLAAMRETDLDEVLAIEKAVYSHPWTRGNFADSLC
                     SGYACRTWRAGGALIGYFVLLAAAGEAHLLNLSIAAAWQRRGHGTALLAEAMRIGRDM
                     GARKIFLEVRPSNLPGQALYERHGFRKIAQRRNYYPAPNGREDALVLSRAL"
BASE COUNT         2549 a         6402 c         5936 g         3039 t
ORIGIN      
        1 gcaacagcga ctcgccgata tggaaacgcg ggtgccgcga cttctcgaac agcgccacgc
       61 gaaaaccccg ctcggcaagc agcgcggcga cggtcgagcc ggccggcccg ccgccgatca
      121 cggcgacgtc gcagtccttc attgcggccg cgccgccctc acccatgccc tttcctgcgg
      181 acatgccgca ttctaccgtg cgcggctagg gcttcttttc caggtttctc tgccggatct
      241 cgatatcggc gcgcgcgcgc agactctcga cgaaggcgtc cagctggcgc gcgccggcga
      301 gctgtgcggc gcgcgcgcgc tccgcggtcc tctgcgcctc ggtcttcggc tcgggctcga
      361 gcaccttcac gacgcggtac agcatgtagc cttcggtgcc gcgcgcggcg ccggcgtacg
      421 ccggcaactt ggccgggtcc gccgccatga tctcgcgcag cgctccggcc ggcacttctt
      481 gcgggctgcg tcgcgacacc gcctttgctg cgccccagct cacctccgac ttgccgtcct
      541 tgcgcagcgc ggcgagcttc gactcgccgg cctgctgcgc aaggcgcgcc gcatcctgcg
      601 aacgcaagcg gcgctcgatg tcggcgcgca cgtcctcgag cttcttctgc gatgcgggct
      661 ggtgctcgat gacgcgcgcg gaaaccagga cgttcggcgc cgcctcgatg gcgtcggtat
      721 tgcggcgcgc cttgacggca tcgtcggcga acagcgcacc ctgcagcttg gggctggcga
      781 gcacaccggc ctccggcgcg ggcacggcgc gcgagatcca gccgctggtc tgcaacggca
      841 gcttgaagcg ctcggcgacg ggcttcaggc tgtcggactg ctcgtagacc atgttgctga
      901 acgtctctgc agcctcggcg aagcggcgcg cgcccagctg gcgcgagagt tccgccgcaa
      961 gctcgtcccg gacctcggcc aggggccgca ccttggcggg ctggatgccc gtcagccgga
     1021 tcaggtgcca gccgaaatcg ctcttcaccg ccgcgctgat ctcgccttcc cgaaggcgga
     1081 atgccgcttc ctcgaaggcc ggcaccatca tgccgcggcc gaagaagcca aggtcgccgc
     1141 cgtccgcggc ggagccggga tcctgcgaat gcttctttgc cagctcggcg aagcgccccg
     1201 ggttcttgcg cacctcggcg gcgagcttgg ttgcctcggc ctcgctctgc accaggatgt
     1261 ggctggcgcg gcgctgctcg tccacgcggt actggctggc gcgcgcctcg taggcagcct
     1321 tgaggtcggc ctcgctcacc gcgtcgcgcc ggccgagcgc ctcggccgag agcacgaggt
     1381 actcggcacg cacgcgctcg ggcacgctga attccgccgg gttggactcg tagtaggcct
     1441 tgatctgggc atcactcacc ttgacctcag cgaggaatgg ctgcgccggg accagcgcct
     1501 cctgcacctc gcgacgctcg gcctggatcg cttccagccg ctcggtgacg gcgcgcgaag
     1561 cgatggcggt gctgcgcacc gactcggtaa gctggttgag cgccatgtcg tggcgcaggc
     1621 gcgcctcaaa ggtctgcggc gtcatgttct gcgcccgcag cagcgcctcg tagttggcgc
     1681 gcgagaagcc gttctcgccc tggaacgcgg gcaccgcggc gatggtctcg cgcagctgct
     1741 cgtcgctcac cgagagcccg ccgcgcagcg cggcgtccgc cacggcgcgc tgcgacacca
     1801 gcgagtcgac gagcgccagc cgcgattcgg tggtgtccag cgcctcggcg tcgatgttcg
     1861 ggccgaacgc cgcgcgcagc cgatcctgct ggcggcgcag ctcttcggcg aactcggcct
     1921 gcgtgatggg cgagccgttc accgtggcga catcgtcgcg cccgccgacc gagcgcgtat
     1981 aggactccag gccgaaaaag gcgaacggga tcaccagcag gactagcacg acctgcgcca
     2041 ggcgcttgcg cttttgtatg agctcgaaca ttcagctaag gattcagcta aggagttggc
     2101 ggagcggacg ggactcgaac ccgcgacccc cggcgtgaca ggccggtatt ctaaccaact
     2161 gaactaccgc tccagcgtgg ctgtggtggg tgctgacggg gtcgaaccgc cgacattcgc
     2221 cgtgtaagag cgacgctcta ccaactgagc taagcacccg cgcaaagggg cgctagttta
     2281 ctgcgtcctt gagcgccttg ccagcgcgaa agcgcggcac gcgcgccgcc gcgatctgca
     2341 gcggcgcacc ggtacgcggg ttgcgccctg tgcgcgcctg gcgctcgccc gggtagaagg
     2401 tgccgaagcc cgccagcgtc actgcctggc ccttcctcaa gttcgatttc accgcgccga
     2461 ccagggcgtc cacggctctt tccgcggcga gcttggagat ctcggcctgc agcgcgatgt
     2521 gctcgatcag gtccgccttg ttcacggcgg cacctcaatg cttgacggcg gtcgccgtcc
     2581 ccgccttgcc gtccgcggac ggcagcggcg ccgccgcttc gggcgccggc agcggctcgg
     2641 gctggcgctc gagcgccact tccagcacct tgtcgatcca cttcaccggg atgatctcga
     2701 gccggttctt gacgttgtcc gggatgtcga ccaggtcctt cacgttctcg tccgggatca
     2761 gcaccgtctt gatgccgccg cggggcgccg cgagcagctt ctccttcagc ccgccgatct
     2821 gcagcacctc gccgcgcagc gtgatctcgc cggtcatggc gacatccgcc cgcaccggaa
     2881 tgccggagag caccgaaacc atggcggtgg cgatgccgat gccggcgctg ggaccatcct
     2941 tgggcgtcgc gccctcgggc aggtggatgt ggatgtcgtt cttctggtag aagtcctcct
     3001 gcacgccgag cttgcgggag cggttgcgca ccaccgagag cgctgcctgg atcgactcct
     3061 gcatgacttc gcccagcttg ccggtggtga tggtcttgcc cttgcccggc atggtcaccg
     3121 tctcgatggt gaggagctcc ccgcccacct cggtccaggc cagtccggtc acctggccca
     3181 cctggttctg cttctccgcc atgccgaagc tgtacttgcg cacccccagg aacttgtcga
     3241 ggctcttcgg cgtcaccgtg atgcggcgcg ccttgccttc gcccgattgc ttgtccgtga
     3301 ggagctgctt caccgctttg cggcagatct tggacacctc gcgctcgagc gcccgcaccc
     3361 ccgcctcgcg cgtgtagtag cgcacgatgt cgcggattgc ggcctcggat accgagagct
     3421 ccccttcctt cagcccggtg ctctgcatct gcttgggcag caggtagcgc acggcgatgt
     3481 tcaccttctc gtcctcggta tagcccgaga ggcggatcac ctccatgcgg tcgagcaacg
     3541 gcgccgggat gttcagcgtg ttcgccgtgg cgacgaacat cacctccgac aggtcgtact
     3601 cgacctcgat gtagtggtcg gtgaacgtgt ggttctgttc cgggtcgagc acttcaagca
     3661 gcgccgagga cggatcgccg cgccagtcct ggcccagctt gtcgacctca tccaggagaa
     3721 acagcggatt cctcaccccc accttggtca tgttctgcag gatcttgccc ggcatcgagc
     3781 cgatgtaggt gcgccggtgg ccgcggatct cggcctcgtc gcgcaccccg ccgagcgaca
     3841 tgcgcacgaa cttgcggttg gtcgcgcgcg ccaccgactg gccgagcgag gtcttgccca
     3901 cgcccggggc gcccaccagg cacaggatcg gcgccttcat cttgtcgacg cgcgtctgca
     3961 ccgcgaggta ctcgacgatg cgctccttca ccttctccag cccgtagtgg tccttgtcca
     4021 ggatctcctg cgcggccttg atgtcgcgcg agatcttgga gcgcttcttc cacggcaggt
     4081 tgaccagcgt ctcgatgtag ttgcgcacca ccgtcgcctc ggccgacatc ggcgacatga
     4141 gcttgagttt cttcatctcg gcttccgcct tgcgccgcgc gtccttgtgc atgtgtgcgc
     4201 gcttgatgcg cttttccatc tcctcgaggt cggcgccctc ctcgccctcg cccagctcct
     4261 tctgtatcgc cttcacctgc tcgttgaggt agtactcgcg ctggctcttc tccatctggc
     4321 gcttgacgcg cccgcggatg cgcttctcca cctgcaggat gtcgatctcg gtctcgagct
     4381 ggctgagcag gtgctcgagc cggctcttca cgtcgaacat ttccagcacc tgctgcttct
     4441 gctcaagctt gagcggcagg tgcgcagcca cggtgtcggc gaggcgcccg gcctcgtcga
     4501 tgcccgagag cgaggtcagg atctcgggcg ggatcttctt gttgagcttc acgtactggt
     4561 cgaacgcaga tagcagcgca cggcgcatgg cctcgatctc ggcgggcgcc gcgccctcga
     4621 tggcgatcgg ctcggcctcg gcgatccagt gcgacttcag gtcggtcgcc gagcggatgc
     4681 gcgcgcggct cgcgccctcg accagcacct tcaccgtgcc gtcggggagc ttgagcatct
     4741 gcaggatgcc cgagacgcag ccgatctcgt acatgtcttc ggggcccggg tcgtccttgg
     4801 cggccgactt ctgcgccacc agcaggatcg acttgcccgc ttccatggcg gtttccatcg
     4861 ccttgatcga cttggggcgc ccgacgaaca gcgggatcac catatgcgga aacaccacca
     4921 cgtcccgcag cggcagcagc gggtactggg tcagtccgga gggggattcg ttcatctcgg
     4981 gcatgtgagg tttcccggag cgaaatcaat ggggccagcc tacacgcaac gcgtgaggct
     5041 cgcaatgcga aaactgcaac gaaaagcggc gcttaggccg acgagccggc aactttttgc
     5101 tggtcggagt agatcagcag cggcttgccg tcagccgaga tcatctgctc gtcgatcacc
     5161 accttgctga cattttccag cgtcggaagt tcgtacatcg tctcgagcag cacgttttcg
     5221 aggatcgagc gcaggccgcg tgccccggtc ttgcgggcga gcgcgcgccg cgacaccgcg
     5281 ccgagcgccg ccgtgcgcac ctcgagctcc acgccttcca tcaagaacag gcgctggtat
     5341 tgcttgatga gggcgttctt cggctcggtc aggatctgga tcagcgcgtg ctcgtcgagc
     5401 tcctcgagcg ccgcgaccac cggcagccgc ccgacgaact cggggatcaa cccgtacttg
     5461 atcaggtcct cgggctcgac cgccttcagc aggtcgaagg tggccttgga gttgccgggc
     5521 gagcgcactt cggcgccaaa gccgatgccc gtcttctcgg tgcggttgcg cacgatcttc
     5581 tccagcccgt cgaacgcgcc gccgcagatg aacaggatgt tggtggtgtc gacctggacg
     5641 aagtcctggt tggggtgctt cctgccgccc tgcggcggca ccgaggcgat cgtgccctcg
     5701 atgagcttca gcagcgcctg ctgcacccct tcgcccgaga catcgcgggt gatcgagggg
     5761 ttgtcggact tcctcgatat cttgtcgatc tcgtcgatgt agacgatgcc gcgcttggcc
     5821 ttgtcgacgt cgtagtcgca gttctgcagc agcttctgga tgatgttctc gacgtcctcg
     5881 ccgacgtagc cggcctcggt cagcgtggtg gcgtcggcga tgacgaacgg cacgttcagc
     5941 agccgcgcga gggtctgcgc gagcagtgtc ttgcccgacc cggtcgggcc gatgagcagg
     6001 atgttggact tggcgagctc gacctcgtcg ttcttggata ggtgcttgag ccgcttgtag
     6061 tggttgtaca ccgcgaccga aaggatcttc tttgcctggt cctggccgat cacgtactgg
     6121 tcgaggatat tgcggatctc gtgcggggtg ggcaggtccg agcgcgcgcc cttgccgccc
     6181 ttatccgagg cggtctcctc gcgaatgatg tcgttgcaca gctcgatgca ctcgtcgcag
     6241 atgaaaaccg acggacccgc gatgagcttc ctcacctcgt gctggctctt gccgcagaac
     6301 gagcagtaca gcagcttgtc gccggcggcg ggcttgtccg acacctaggc gggctcccga
     6361 gtggtgagta ccttgtccac gaggccgtac tttaccgcgt cttcggcccc aaggaaaaaa
     6421 tcgcgctcgg tgtcgcgcgc gatggtctcc acgggctttc cagtgtgctt ggcgaggatc
     6481 tcgttcaggc ggccgcgcag gtacaggatc tccttggcgt ggatctcgat gtcggtggcc
     6541 tggccctgaa agccgcccat cggctggtgg atcatgacgc gcgagttggg caggacgaag
     6601 cgcttttcct tgtcgcccgc ggcaagcagc aacgacccca tgctcgcggc ctggccgacg
     6661 caaagggtgc tgacgtcggg cttgatgaac tgcatggtgt cgtagatggc gagccccgcc
     6721 gacaccgagc cgccgggcga gttgatataa aagaagatgt ccttgtccgg gttctcggac
     6781 tcgaggaaca gcagctgcgc gacgatcagg ttggcggtcg actcggtcac cacgccgacc
     6841 aggaacacca cccgctcctt gagcagccgg gaatagatgt cgtaggcacg ctcgccgcgc
     6901 cccgactgct cgatgaccat cgggatcaaa ccaaggtcat tcgcttccgg catgcggttg
     6961 tgccagttca tgcgttgctt cccataagct cgtcgaaggg cattgccttg tccaccacct
     7021 tggccttctg cagcacccat tgcaccacgt tggcctcgag cgccaggccc tccatctccg
     7081 acaggcgctg cggctgcatg tagaaccatt ttactacctc ggcgggactc tcgtacgact
     7141 gcgcctcctg ctcgatcagg gcgcgcacct cggccggctt gggctgcagc ttctcggcac
     7201 gcgccagctc ggcgatgatc agcccgagcg ccacgcgccg cttcgcgctc gcctcgaacg
     7261 cctgcgggtc gagcggcagc ttctcgaact tcaggccgcg cgcgcgcagg tcggcggcgg
     7321 cgcgctccac cagctcgcgc gtttccatgg cgaccagcga cttcggcagc tcgagcggcg
     7381 tcgcctcgag cagcgcctgc atcgcctgct gcttcacccg cgcctcgatg cgcttggccg
     7441 tctcgcgctc cacgttctgc ctgacctccg cacgcatctt cgccaggtcg ccgtcggcca
     7501 cgcccagtgc cttggcgaag tcggcatcga gcgccggcag ccgcggttcc tcgaccttct
     7561 tcaccgtcag cgcgaactcg gcggtcttcc cggcgacttc cttgccgtgg tagtccggcg
     7621 ggaatgtgac ggagaagttc ttcgcttcgc ccggcgctgc gccgcccgcc gcagcctcga
     7681 actcgggcag catgcggcct tcgccgagca cgaaggcgaa gccgtcggcc ttgccgccgg
     7741 ggaacggctc gccgccgatc gtgccggcga aatcgacgat gacgcggtcg ccggactgtg
     7801 cggcgcgggc cgcttcggcg taggtcacgc gctgcttgcg caggatctcc agggtctggt
     7861 ccaccgcggc gtcgtccacg ctgacctgcg gccgctcgac ggtggcggcg gcgacgtcgc
     7921 cgatcctgaa ctcggggtac acctcgaacg tggcgctgaa ctccagcgcg tccgcggcgg
     7981 cgtccttctt cggctcgatg cgcggctggc cggcgacccg caggttcgcc tcgcgcaccg
     8041 cgtcgctgaa cgccttctgc acggcgtcgc cgagcacctc cgagcgcacc tgcggcccgt
     8101 aggtctgcgc cagcaccttc atcggcacct tgccgggccg gaagccgggc atcttcgcgt
     8161 tgcgcgcgag cttcttcagg cgctggtcga tctcctgctc gatgcccgac aggggcacct
     8221 tcatctgcaa gcgccgctcc agcgcaccga gttgttccac gtttgccgcc atgcccgtct
     8281 agatccttcc gtgttgtgcg ctggtgcgaa gggagggact cgaaccctca tgccttgcgg
     8341 cgtcagatcc taagtctggt gcgtctgcca attccgccac cttcgcgcga aaaagcgaat
     8401 ggaaagaaag tattatacct gcgcgtgtcg gtcgctcatt acgagaactt ccccgtcgcc
     8461 tcgctgctgc tgccggcgcc gctgcgcgcg ccggtggcgg tgatctatcg cttcgcgcgc
     8521 ggcgccgacg acatcgccga cgagggcgac gacgcgccgc aggagcgcct gcgcaagctg
     8581 gaggagttcc ggcggcagct cggcgccccc gccgcaccgc tgttcgagga cgtcgcgcgc
     8641 atcgtggccg agcacgcgct gccgcggcag ctgttcggcg acctgctcga cgcgttcgcg
     8701 caggacgtga ccaagacgcg ctacgccgac tacgccgagc tgcgcgatta ctgccgccgc
     8761 tccgccaatc cggtgggacg gctgctgctg cacctgttca agcgaacgtc tgccacggac
     8821 ctgcagcgct ccgacgccgt ctgcaccgcg ctgcagctga tcaatttctg gcaggacgcc
     8881 gcgctcgatt acgccaaggg ccgcatctac ctgccgcagg acgagatggc gcgccacggt
     8941 gtcaccgagc gccatctggt ggaggcgcgc tgcgacgacg cctggcgcgc gctgatggcc
     9001 tttcaggtgc agcgcacgcg cgcgctgatg ctctcgggcg cgccgctcgg gcgcgcgctg
     9061 ccgggacggg tgggcctgga gatccgcgcc acggtgcagg ggggcctgcg catcctggag
     9121 aagctcgagc gcgccggcta cgacgtgttc cgccgccgtc cggtcctccg cgggttcgac
     9181 tggctgctcg tcgccgcgcg cgcgctgtga cgcccgacgc ctactgccag caaaaggccg
     9241 ccgccagcgg ctcgagcttc tactacgcat tcctgttcct gccgccagcg cgccgccgcg
     9301 ccatcaccgc gttgtacgcg ttctgccgcg aggtggacga cgtggtcgac gagacctcgg
     9361 acgcgcagct cgccgccacc aagctcgcgt ggtggcgcca ggaagtggga ttgctgtttg
     9421 cgggcacgcc gcagcacccg gtgtcgaagg cgctcgcgcc cgccatcgcc gcctacggca
     9481 tcaccgccca gcgcctgaac gagatcatcg acggcatgca gatggacctg acgcagacgc
     9541 gctatctcga tttcgccgcg ctcgagcgct actgctacca cgtcgccggc gtggtcggca
     9601 tgctagcggc cggcattttc ggctacagcg atccgcgcac gctggactac gcgcgctacc
     9661 tcggcacggc gttccagctc accaacatca tccgcgacgt gggcgaggat gcgcgcaaga
     9721 accggattta tctgccgatc gccgacctgc agcagttcgg cgtcgccgcc gcggacttgc
     9781 tgcaggcgcg ccatagcgac gcgttcgtgt cgctcatgcg cttccaggcc gagcgcgcgc
     9841 gcggctttta ccgcgaggcg ctggcggcgc tgccccgcgc cgaccgccgc gcgcagcgcc
     9901 ccgggctgat catggcggcg atctatgcgg cgctgctcga cgagatcgag cgcgacggct
     9961 ttcaggtgct gcgcgcgcgt acctcgctca cgccgctgcg caagttctgg atcgcctgga
    10021 agacgtggct cgccgtatag ccgttgtcgg tgccggctac gccggcctgg cggcggcggt
    10081 aacgctcgcc aagcgcggcg ccgcggtcac cgtcttcgag tccggtcccg tgccgggcgg
    10141 acgcgcgcgg cgcgtcgaga gcggcggcga ggcgctcgac aacggccagc acatcctcat
    10201 cggcgcctac gacacgctct ttggcctgat gcgcacggtg ggcgtgccct ctgacgcggt
    10261 gctgcgcatg ccgctcgagc tgcgctatgc cgacggcgtg tcgctgcggc ggctgttcct
    10321 gcccgcgccg ctcgggctgc tcggcgggct gctgctggcg cgccgcctgc cgtggcgcga
    10381 gaagctcggc gcggtgcgtt tcatggcggc gctgcggcgg atcaacttcc ggctggccga
    10441 ggattgcagc gtgagcgcgc tgctcgcgca gcaccggcag gacggccgca tcggccatta
    10501 cgtctggcag ccgctgtgcg tttccgcgct caataccccg ccggcgcagg cctcggcgaa
    10561 cgtcttcctc gccgtgctgc gcgatacgct tgccggccag gccgaggcga gcgacctgat
    10621 cctgccgcgc atcgacctgt cgcggctgtt tcccgagccg gcgatggcgt ggcttcaaca
    10681 gcggggtgcc gaagtgcgct gcgggacgac ggttcgagac ctggatccac tgcgtgccga
    10741 gcacgcggcc gtgatcgtcg ccgtcggccc gcaccagttg aagaccctgc tgccgcggtc
    10801 ggcgcccgag ttccagtacc agccgatcac caccgtctac ctgcagtacg gcccgaagac
    10861 gcggctgccg ctgccgatgc tcggcctcgc cggcggcctg gtgcagtggg tgttcgaccg
    10921 cggcgcgctc accggcgaaa aggggcgcct cgccgccgtc atcagcgcgc agggcgagca
    10981 ccagcagctg acgcaggacg agctcgcgcg cgcctgccac cgcgagctcg ccgctgcgct
    11041 gaagaacctc cccgagccga aatggtcgcg cgtcatcgcg gaaaagcgcg ccaccatcgc
    11101 agtggcgccg ggcttgcgcc gccccggcat cgaaacgccc cttccgggcg tgttcctggc
    11161 gggcgactat accgatcccg agtatccgcc gacgctcgaa gccgcggcgc gcagcggcgt
    11221 gcgcgcggcg gaacgcgcgc tggtcgacgc ataaaggagc atagatggaa taccggcaac
    11281 tgggcgccag cggcttcatg gtcccggcgc tgactttcgg caccggcacc ttcggcggcg
    11341 gcggcgagtt cttcaaggcc tggggcgcaa cggacgtcgc cgaagcaaag cgcctcatcg
    11401 acatctgcct ggaggccggc gtcaccatgt tcgattcggc cgacgtgtac tcgggcgggc
    11461 tggccgagga gatcctcggc aaggcggtcc aggggcgccg cggcgagctg ctcatctcca
    11521 cgaaaggcag cttccggcac ggccccggcc ccaaccaggt cggctcgtcg cgccagcacc
    11581 tcattgctgc ggtcgaggcg agcctgacgc gcctgggcac ggatcacatc gacctctatc
    11641 aattgcacgg cttcgacgcc atgacgccgc tcgaagaagt gatgcagacg ctcgacggcc
    11701 tggtgcgcgc gggcaagatc cgctacatcg gctgctccaa cttctccggc tggcacctga
    11761 tgaagtcgct cgcggtgtcg gccagatacg gccttgcgcg ccacgtcgcg caccaggcgt
    11821 actactcgct cgtcggccgc gactacgagt gggagctgat gccgctcgcg ctcgaccagg
    11881 gcatcggcac cgtggtctgg agcccgctcg cctggggccg gctcggcggc aagttccgcc
    11941 gcggccagcc cctgcccaag gacacgcggc tcgcctcgcc ggcgaccgcg caacgcggcc
    12001 cgcaggttcc cgacgagtac ctgtaccgcg tggtggacgc gctcgacgcg gtggccgcgg
    12061 aatgcggcaa gagcgttccg caggtggcgc tcaactggct gctgcagcgg ccgtcggtga
    12121 cctcgatcgt catcggcgcg cgcaacgagg cgcagttgcg cgacaacctc ggcgccgtgg
    12181 gctggaagct gacaccggcg cagatcgcca agctcgacgc cgcgagcgcg gtgccgctcc
    12241 cctaccccta ctggcaccag cgcgcgatct acggcgagcg caacccgccg cccgtatgac
    12301 gcgcttcgag gcactgcacg cagacatcac cacgctcgag gtggacgcga tcgtcaacgc
    12361 ggccaatgag ggcctgctcg gcggcggcgg cgtcgacggc gccatccacc gcgccgccgg
    12421 gccgcagctg ctcgaggcat gccgggccct ggccgggtgc gcgacgggcg aggcgaagat
    12481 cacgctcggc taccgcctga aggcgaggca cgtcatccac accgtgggcc cggtatggcg
    12541 cggcggcaat cgaagcgagc ccgccctgct cgcctcgtgc taccggcgct cgctggagct
    12601 tgccgcccag cacgcgctcg agagcattgc ctttcccgcg atcagcaccg gggtgtacgg
    12661 ctatccgaag gaagacgcgg cccgcgtggc ggtcgcgacc gtcgccacgc acgcgacggg
    12721 gcagctgcgg cgggtgatct tctgctgctt ctcggcgcag gacgaggcgc tctacgccgc
    12781 gctgctcaga gatcccgcag cagccgcacc gcctggtcca cccgctcgac cgcgatgacc
    12841 tccagcccgg cgatgcggcc cttgggcgcg ttcgcccgcg gcacgacggc gcgctcgaag
    12901 ccgagcttcg ccgcctcctt cagccggtcc tgaccgcgcg gcgcgggccg cacctcgccg
    12961 gcgaggccga cttcgccgaa ggcgaggagc ttgcccggga tcggccggtc ggtgagcgaa
    13021 gacacgatgg cgagggcggc ggcgaggtcg gccgccggct cgccgatgcg cacgccgccc
    13081 accgcgttca cgaacacgtc ctggtccatg gtggcgatac ccgcgtgccg gtgcaggacg
    13141 gcgagcagca tcgccaggcg gttctgctcc aggcccacgg tgaggcggcg cgggttgggc
    13201 gcatgcgcgg cgtcgaccag cgcctggatt tccaccagca gcggccgcgt gccttcgagc
    13261 gtcgccagca cgcaggaccc cgcgacgtcc ttttcatgcc gctgcaggaa cagcgccgac
    13321 gggttcgcca ccgcacgcag cccgctgtcg gtcatcgcga acacgccgag ctcgttcact
    13381 gcgccgaagc ggttcttcac cgcgcgcacc aggcggaacg ccgaatgcgg gtcgccctcg
    13441 aaatacagca ccgtatcgac gatgtgctcg agcacgcgcg ggcccgcgat cgcgccttcc
    13501 ttggtgacgt gcccgacgat gaacagggcc gcgcccgcct tcttcgcgtg gcgcgtgagc
    13561 tgcgcggcgc attcgcgcac ctgcgccacc gagcccggcg ccgactgcag cgtgtccgac
    13621 cacagcgtct ggatcgaatc caccactgcc acctcggggc gcgcggcctg cagcgcgccg
    13681 agcacgcgct cgagctggat ctcggcgagc aagcccacgc ccgcggcgcc caccccaagg
    13741 cgccgcgcgc gcagcgccac ctgctcggcg gattcctcac ccgagacgta cagcaccttg
    13801 cgcttcgcgc cgagggcggc gagcgcctgc agcagcagcg tcgacttgcc gatgcccgga
    13861 tcgccgccga tcagcgtcac ctgcccggca accagccccc cgccgagcac gcggtcgagc
    13921 tcgggcaggc cggtgcccac gcgctcgacc tcgcgcgcct cgacctcggc gagcggcacc
    13981 gggtgcgccg ccggcccgct ggcgcggccg ccgcgctcgg cggcctgctc gctcatcgtg
    14041 cccgcctcgc cgcaggaggg gcacaggccc atccacttca gcgccgtcgc gccgcactcc
    14101 gcgcacacga acaccgactt cgccttcgcc atcaggcctc ctcgcgcacc ggcacgcgcg
    14161 gcgcgagcgc gcacagcagc tcgtagctca cggtgccggc ggctgcggcc acttcgtcgg
    14221 caggcagccc ctcgccccac agcgttaccg gcgtgccgat gtaggcctcg tcgatgccgg
    14281 tgatgtccac gcacatcatg tccatcgaca cccgccccac gaggcgcgtg cgccggccct
    14341 tcaccagtac cggcgtgccg gtgggcgcgt gccgcggata gccatcggcg tagccgcagg
    14401 ccaccacccc gagtgtcatc ggttccttcg cctcgaacgt aaagccgtag ccgacgcgct
    14461 cgcccgcggc caggttctgc actgcgatca gctccgaggt cagcgtcatc gcgggctcca
    14521 ggccgatctc ggcggcgctc cagtcggcaa acggcgagca gccgtagagc atcagccccg
    14581 ggcgcaccca gccctcgcgc gcctccctga acctgagcag cgccgccgag ttggcgaacg
    14641 aacgcggcgc ctcgatgccg cgcaccagct cctcgaacca gcgcagctgg ccctgcacac
    14701 cgctctcgcc ctcggcgtcg gcgaaatgcg tcatcagcgt gatgcggccg atgttcgggt
    14761 gcaggcgcag cgtgttgtac atgacgcgca cctcatcgat gccgaagccg agccggttca
    14821 tgccgctgtt gaccttgagg tagacgtcga gctcggcggg gaaatccatg tgcttgagca
    14881 gctcgacctg gtcggggttg tggatcaccg gcgtcaggcg gtacttgaac agcaggttga
    14941 catcgagcgg ccggaagaac ccctccagca tgaggatcgg cttggtcacc cccgcaaggc
    15001 gcaggcgcac cgcttcctgg aagtcgagca gcgcgaagcc gtcggcgccc tcgagggcgc
    15061 gcgcggcgcg ctccaggcca tggccatagg cgttcgcctt caccaccgcc cagatcttcg
    15121 cctcgccggc ggcctcgcgc gccacctgca ggttgtggcg cagggcggac gccttgacga
    15181 cggcgcggat cgggcgcggc atggggccgt aagcatagcc taccgctcgg ccatgctata
    15241 agaccgcagc catgagcctc ggcttctaca tcatcatggc agcgcagttc ttctcgtcgc
    15301 tcgccgacaa tgcgctgctg gtggcggcca ttgcgctgct catgctgggc gactcgccca
    15361 cctggctcac gccttacctc aagttcttct tcgtcatctc gtacgtcgcg ctcgcgcctt
    15421 acgtcggcgc cttcgccgac cgcctgccca aggggcacgt gatgttcgtc tccaacagca
    15481 tcaagatcgt cggctgcgcg ctgatgctgt tcgaggtgca cccgctcgcc gcctacgcgc
    15541 tggtcggcct gggcgccgcg gcgtactcgc cggccaagta cggcatcctc accgagtacc
    15601 tgccgccgac caagctggtg atcgccaacg gctggatcga gggccttacc gtcggctcga
    15661 tcatcctcgg cacggtgctg ggcggcgtgc tgatcagcgc acgcgtctcg gggcacctgc
    15721 tcggctacga cctgccgctc gtcgatctcg gcatcgacaa cgcggccgaa gcggccattt
    15781 cggtgatcgt cttcgtgtac gcgacggccg ccctgttcaa cctgtacgtg ccgcgcaccg
    15841 gcgtggcgct gaagccgctg ccgcccgacc cgctcgcgac gctgcgcgac ttctaccact
    15901 gctgccagcg cctgtggacc gaccgcctgg gccagatctc gctcgcggtg acgacgctgt
    15961 tctggggcgc cggcgccacg ctgcagttca tcgtcatcga gtgggcgcgc gtcgcgctcg
    16021 actacaacct gtcgcgcgcc gccatcctgc agggcgtggt ggcgctgggt atcgccgccg
    16081 gcgcggtcgt ggcgtcgctg cgcgtctcgc tcgaccgggc cgtcggcgtg atccccctcg
    16141 gcatcggcat ggggctggtg gtcatcgtca tgaacttcgt cagcagcatc ggcatcgcca
    16201 tcccgctgct catcctgatg ggcgcgctgg ccggctactt cgtggtgccg atgaacgcgc
    16261 tgctgcagca tcgcggccac aacctgatgg gcgcggggcg ctcgatcgcc gtgcagaact
    16321 tcaacgagaa cgcctccatc ctgatcatga tcgctgtgta ctcggcgctg ctcggcgccg
    16381 ggctctcgat ctacgtggtg atcgtgatct tcggcctgtt cgtggccggc accatgacgc
    16441 tggtgctcct gtggtaccgg cgcaaccgcc gcgtgcaccg cgaggagatc gaggcgctgc
    16501 tggagatcgc gcggtcggag aagcactgag caagcgacga agcgcgcgac gtcgcgaatt
    16561 tgaccttctc gggtctcgcc ttgttcggcc ggaagattca cccggccgaa aaaggcgaga
    16621 tgtaataaaa ccaaacccgg cactacctca actcgaggcg attgttctcc gcgcaaaaac
    16681 cagatccgcc cacgctttgg ccttgtccgg caggttcctg agcagatacg cggggtgata
    16741 ggtcaccacc agcggcacgc cgtgatactg atgcactttg ccgcgcaggc tgccgatggt
    16801 ggcgtcggta ttgagcagcg tttgcaccgc gaagcgcccc atcgccacga tgagcttggg
    16861 cctgatcagg tcgatctggc gcagcaggta cggcgagcag gttgcgacct ccggcggctc
    16921 gggattgcgg tttcccggcg gccggcactt gagcacgttg gcgatgtaga cgttgttgtc
    16981 gcgcgcgagg ccgagcgcgg cgagcatgtt gtcgaggagc ttgccggcct gcccgacgaa
    17041 cggctcgccg agccggtctt cttccgcgcc cggggcttcg ccaacgagga gccagttggc
    17101 attctcgtcg ccgacgccga agacagtttg cttgcagccg gcgcgcaggc cgcaggcggt
    17161 gcaattgtgg acggccgcct tgagctccgg ccactgcatc ctcgcgactt gctcttccct
    17221 cgatcccccg gtttccgcgg ttgttgtcgc cgcggaaacc gggggattct tttcctgccg
    17281 caaccgccag acgggaccca gtcccatctc ctccaggatc tccagcctcc ggctcacagc
    17341 gccctgctca gcaccagcgc atcctcgcgc ccgttcggcg ccgggtagta gttgcgccgc
    17401 tgcgcgatct tcctgaaacc gtggcgctcg tagagcgcct gccccggcag gttgctcggc
    17461 cgtacttcca ggaagatctt gcgcgcgccc atatcgcggc cgatgcgcat cgcctcggcg
    17521 agcagcgccg tgccgtggcc gcgccgctgc caggccgcgg cgatggacag gttgagcagg
    17581 tgcgcttcgc ctgccgccgc aagcagcacg aagtagccga tgagagcgcc gcccgcgcgc
    17641 caggtgcggc aggcgtagcc cgagcatagc gagtcggcga agttgccgcg cgtccacggg
    17701 tggctataga ccgctttctc gatcgccagc acttcatcga ggtcggtctc gcgcatggcc
    17761 gcgagccgcg gaaggtcctt gaggacggcg ctcatcggcg gccgcgctcc tcggtggtca
    17821 gcgcaacctt gtcgcgcagg tacaccggcg cgatgcgcgc ggcgtcgacg ccctcgcccg
    17881 cggcgagccg cggcgcggca agccgcgcca ccgccgccgc gccggg
//
LOCUS       DBNHKE010000098         9240 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1338532, whole genome shotgun sequence.
ACCESSION   DBNHKE010000098 DBNHKE010000000
VERSION     DBNHKE010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1338532"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="AC16O9_06065"
     CDS             <1..688
                     /locus_tag="AC16O9_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR6516215.1"
                     /translation="LLRHAADEGALKTLKAGVLVAAVLAPLQIFVGDLHGLNTLEHQP
                     AKIAAVEAIWSTEKGVPLVLFAIPNQLAGRNDYSIEVPKLASFVLKHDFDAELQGLDA
                     FAPDHPPVAPVFFAFRIMVGMGVLMLGLAWLGAWWLRKRAPPRWLLWGFAGMTFSGWV
                     ATLAGWIVTEIGRQPWLVTGILRTADAAGRVAEASLGASLTAYALVYAVMLLAYMVTL
                     THMAGEGAKA"
     gene            685..1659
                     /locus_tag="AC16O9_06070"
     CDS             685..1659
                     /locus_tag="AC16O9_06070"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR6516216.1"
                     /translation="MSLDLPLIFAVLMGAAILAYVLLDGYDLGVGMLMPAATPDEQDV
                     MVASIGPFWDANETWLVLGVGILLIAFPAAHGYILGELYLPVAAMLVGLMLRGVAFEL
                     RVKAVGWHAELWNWLFWLGSFLASFAQGYMLGRYLTAFQPGWGYGLFSLLTGAALCGG
                     YVLLGAAWLILRTEGELQKKAMAWTRWGLAWVALGVALVSLATPMVSELVYHRWFDFP
                     ATLALMVLPAASLVSWLWVWRSIGRSDWKPLAGAMAIFVLAFLGLAYSVFPYLVLGWL
                     TIWDAAAHPSALKVVLAGALIVLPFLVGYTVFAYRVFRGKAKAGLYGH"
     gene            complement(1726..2535)
                     /locus_tag="AC16O9_06075"
     CDS             complement(1726..2535)
                     /locus_tag="AC16O9_06075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR6516217.1"
                     /translation="MTEATALSAISSTAREWRNRLRLPPKGESMAQDAEWCEIREAGG
                     WRRMRFHDYADIYERPGLYEYLFYDLLACQSPRRVVGLLAEVRAELPQNEPLRVIDFG
                     AGNGLVGKELRRIGAVEVVGVDILPEARAAARRDLPGAYSDYLVADMTRPDEDLDRRL
                     RALRPNALTCVAALGFGDIPPAAYHRAAGYVSIGGLLAFNIKEEFLDVRYTHGFSEMM
                     RRMLNDGIIRLEATRRYRHRLSASGEPIYYTAMVATKLKEIPDGILVEEQQ"
     gene            complement(2697..3020)
                     /gene="fdxA"
                     /locus_tag="AC16O9_06080"
     CDS             complement(2697..3020)
                     /gene="fdxA"
                     /locus_tag="AC16O9_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR6516218.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVPECPVEAIFAEDDVPDGQREWIAKNKALAAGWQPIIERKPAPADADEWAKVKEKK
                     HLLES"
     gene            3140..3472
                     /gene="trxA"
                     /locus_tag="AC16O9_06085"
     CDS             3140..3472
                     /gene="trxA"
                     /locus_tag="AC16O9_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HIR6516219.1"
                     /translation="MSSSTQIHHVTDASFEPEVLQSEVPVLVDYWAEWCGPCKSIAPI
                     LDQVAQEYDGRLKIAKLNVDENQAVPAKFGIRGIPTLMLFKNGTLEATRVGALSKSQL
                     TAFLDSNI"
     gene            3629..4888
                     /gene="rho"
                     /locus_tag="AC16O9_06090"
     CDS             3629..4888
                     /gene="rho"
                     /locus_tag="AC16O9_06090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR6516220.1"
                     /translation="MHLSELKSQHVTQLVETAVANQIDNASRMRKQDLIFALLKNQAK
                     KGESIFGDGTLEVLPDGFGFLRSPETSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNSEVPEAAKNKILFENLTPLHPDEMFKLEREIKAEENLTGR
                     VIDIVAPIGKGQRGLIVSPPKAGKTVMLQHIAHALVANHPEVSLIVLLIDERPEEVTE
                     MSRSVRGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQKPKRFFGAARHVEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMHEKRIFPAINVNRSGTRREELLLQKDVLQKIWVLRKLLFPMDE
                     LEAAEFLIDKLRATKNNAEFFDSMRRG"
     gene            5149..5763
                     /locus_tag="AC16O9_06095"
     CDS             5149..5763
                     /locus_tag="AC16O9_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6516221.1"
                     /translation="MPRALAWAIDLVLRALVVIGVVMVAGYFGRAGWGLALLVMFVVE
                     WLAPAWFEVVWNGQTPGKRALGIAVLNDDGTPVRWSASMTRNLLRAVDFLPFLYGFGL
                     ITMLASRDFKRLGDLAAGTVVVYAPGKARARTLPEAPAEPPPFPLSLEEQRVVLDLAE
                     RSASLTRERLEELASLPVPLTGGASGATAAARLLGMASHLSGRP"
     gene            5760..6725
                     /locus_tag="AC16O9_06100"
     CDS             5760..6725
                     /locus_tag="AC16O9_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:1901893 - positive regulation of cell
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR6516222.1"
                     /translation="MKQAPFEAAHGEEWRAFERFLDAAAGAPPPFAPEEMPRRYRRLC
                     HHLALAADRQYSPELVDRLNLLALRGHHALYANRRRQSQRIVDFLLVAFPSLVRAEWR
                     VVIAAALLFFGPLVGLIAVLQLYPEFVHYLLDPRQIAGFHDMYDPANRRLGMREADTH
                     LAMFGYYIWNNVRIGFQTFAGGLLAGLGTLWYLAANGVILGAVAGYLTQIGFGETFWS
                     FVSGHAALELTAIVISGAAGLRLGMAVIAPGNRSRKAALVAVARPAVRLMYGAALMFT
                     AAAFVEAFWSPLTSVPTAVKIWAGLAGWALLLAYLVLAGRARAAR"
     gene            6712..8178
                     /locus_tag="AC16O9_06105"
     CDS             6712..8178
                     /locus_tag="AC16O9_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR6516223.1"
                     /translation="MQLDRLAIALRPRSAWESVDLGVGMLREWWRPLYASFAAVYLPL
                     AAACFWAGWSTGKAWLAMVMLWWLKPLFDRVALHVVSRAVFGDVLGVRATLAQARAWL
                     ASGLFGALTLGRVDTARSFHLPVKQLEGQGWRAARARRALLGRRTHGYAVWLTLACVN
                     FELVFVWSADMLWNIVVPAKAQEGTSIWEAMFADAAFDNVFSLADFAAYAMAVFIIEP
                     LYVAAGFALYLNRRTLLEGWDLEVALRRIGAQRAAVAATLLLAAALALLAPAAPALAQ
                     APAKDPKREIAEVLKAPEFPHRRTVTRWQRRSSDEAHAGPGWDLSWLRAIGYAFAKSS
                     EVALWIAAGTLVALGLWWALRLMPRAPPATRAPYQPPAALFGMALAPETLPADLAGAV
                     RAHLAAGRVREALALLYRGTLSALVHGRGIELRASDTEGEALARVRAQGEAATAQFFG
                     ELVLHWQRAAYARRPPAAAEVERLLAAYGERLAPRSPA"
     gene            8175..>9240
                     /locus_tag="AC16O9_06110"
     CDS             8175..>9240
                     /locus_tag="AC16O9_06110"
                     /inference="COORDINATES: protein motif:HMM:NF025617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HIR6516224.1"
                     /translation="MSRPLRLALGGLLLVLLAWYLSGLEKVTVTQRVGFSGEARLRPF
                     LAAERFAERMGLAARELRALPELEQLPPNGVLVLPRNRQSIEARRAAQLVLWAAAGNH
                     LIVEAERDGIADPLLAQLRITRADAQVRSPRLTAGVPGNERELKLSGFAAGRLQAEGR
                     APRYRVGPAGEARLITVAHGQGLVSAAVDLNFARNAWIGEHDNAVLLWELLSLHPAGE
                     LLVFHHPRRLSLWDFLVARAPWALLAAAALVALWLWRIAPRYGTVLPDAPPARRRLLD
                     HLRAAGRFLWSHGLRAPLAAAAREAALRRVARAYPDFAHAPRAEQAARLAALAGLAPA
                     DAALLLAAPGAAPAPGGAEFM"
BASE COUNT         1280 a         3280 c         3175 g         1505 t
ORIGIN      
        1 cctgctcagg cacgccgcgg acgaaggcgc gctgaagacg ctgaaggcgg gcgtgctcgt
       61 cgccgcggtg ctcgcgcccc tgcagatctt cgtcggcgac ctgcacggcc tgaacacgct
      121 cgagcaccag ccggcgaaga tcgccgcggt ggaggcgatc tggagcaccg aaaagggcgt
      181 gccgctggtg ctgttcgcca tcccgaacca gctcgcgggc cgcaacgact actcgatcga
      241 ggtgcccaaa ctcgcgagct tcgtgctcaa gcacgacttc gacgccgagt tgcaggggct
      301 cgacgcgttc gcccccgacc acccgccggt ggcaccggtg ttcttcgctt tccgaatcat
      361 ggtcggcatg ggcgtgctga tgctcggcct ggcgtggctc ggcgcctggt ggctgcgaaa
      421 gcgcgcgccg ccgcgctggc tcctctgggg cttcgccggc atgaccttct cgggctgggt
      481 cgcgacgctc gccggctgga tcgtcaccga gatcggccgc cagccctggc tcgtcaccgg
      541 gatcctgcgc accgcggacg ccgcggggcg cgtcgccgag gcgagcctcg gggcgagcct
      601 caccgcctac gcgctcgtct acgcggtgat gctgctcgcg tacatggtga ccctcacgca
      661 catggccggc gaaggagcga aagcttgagc ctcgacctgc cgctcatctt tgccgtgctg
      721 atgggcgcgg cgatcctcgc ctacgtgctg ctcgacggct acgacctcgg cgtcggcatg
      781 ctgatgccgg cggcgacgcc cgacgagcag gacgtgatgg tcgcttcgat cgggccgttc
      841 tgggacgcca acgagacctg gctggtcctc ggcgtgggaa tcctcctcat cgcgtttccg
      901 gcggcgcacg gttacatcct gggcgagctc tacctgccgg tggcggcgat gctcgtcggg
      961 ctgatgctgc gcggcgtggc gttcgagctg cgcgtgaagg cggtcggctg gcacgccgag
     1021 ctgtggaact ggctgttctg gctcggctcg ttcctcgcct cgttcgcgca gggctacatg
     1081 ctcggacgct acctcaccgc gttccagccg ggctggggct acggcctgtt ctcgctgctg
     1141 acaggggcag cgctctgcgg cggctacgtg ctgctcggcg ccgcctggct catcctgcgc
     1201 accgagggcg agctgcagaa gaaggccatg gcgtggacgc gctggggcct tgcctgggtg
     1261 gcgctcggcg tggcgctcgt gtcgctcgcc acgccgatgg tgagcgagct ggtctatcac
     1321 cgctggttcg acttcccggc gacgctcgcg ctgatggtgc tgcccgcggc ttcgctcgta
     1381 tcctggctct gggtgtggcg ctcgatcggg cgctcggact ggaagccgct cgcgggcgcg
     1441 atggcgatct tcgtgctggc gttcctcggc ctcgcctaca gcgtctttcc gtatctcgtg
     1501 ctcggctggc tcacgatctg ggacgccgcg gcccatccct cggcgctcaa agtggtgctc
     1561 gcgggcgcgc tcatcgtgct gccgtttctc gtcggctaca ccgtcttcgc ctaccgcgtg
     1621 ttccgcggca aggcgaaggc cggcctctac ggccactaaa aaatggggac agacctcatt
     1681 tttcttggaa aagaaaaatg aggtctgtcc ccatttcttt tgcggttact gctgttcttc
     1741 gaccaggatg ccgtcgggga tttccttcag cttcgtcgcg accatcgcgg tgtagtagat
     1801 cggctcgccg cttgccgaga ggcggtgccg gtagcggcgc gtcgcctcga gccggatgat
     1861 gccgtcgttc agcatgcggc gcatcatctc ggagaagccg tgggtgtagc gcacatcgag
     1921 gaactcctcc ttgatgttga acgcgagcag tccgccgatc gacacgtagc cggcggcacg
     1981 gtggtaggcc gcgggcggga tgtcgccgaa gccgagcgcc gcgacgcagg tgagcgcgtt
     2041 cgggcgcagc gcgcgcagcc gccggtcgag gtcctcgtcg ggccgcgtca tgtcggcgac
     2101 caggtagtcg ctgtaggcgc ccggcaggtc ccgccgcgcg gcggcgcgcg cctccggcag
     2161 gatatcgacg cccaccacct ccacggcgcc gatgcggcgc agttccttgc cgaccaggcc
     2221 gttgccggcg ccgaagtcga tcacgcgcag cggctcgttc tgcggcagct ccgcgcgcac
     2281 ctcggccagc aggcccacca cgcgccgcgg cgactggcag gcgagcaggt cgtagaacag
     2341 gtattcgtac agccccgggc gctcgtagat gtcggcgtag tcgtgaaagc gcatgcggcg
     2401 ccagccgccc gcctcgcgga tttcgcacca ttcggcgtcc tgtgccatcg actcgccctt
     2461 gggcggcagg cgcaggcggt tcctccattc gcgcgcggtg gaggaaattg cggatagagc
     2521 ggtggcctcg gtcataggcg ttctctcctt tagctgcaat tgcgacgcgc tttgatgccg
     2581 tgcgcgaccg gggagttgct gcactgcggg aactacaggg ccgcacggcc ttgtcatggc
     2641 aaccggcggg cgatggctga gggtaaccga tgcccggacc ccgggcaaac cggggcctag
     2701 ctctcgagca ggtgcttctt ttccttcacc tttgcccact cgtcggcgtc ggccggggcg
     2761 ggttttctct cgatgatcgg ctgccaaccc gccgcgagcg ccttgttctt cgcgatccac
     2821 tcgcgctggc cgtccgggac gtcgtcctcg gcgaagatcg cctccaccgg gcactccggc
     2881 acgcacaggg tgcagtcgat gcactcgtcg gggtcgatca ccagcatgtt cggcccctcg
     2941 cgaaaacagt ccaccgggca cacgtccacg cagtcggtgt acttgcattt gatgcagctt
     3001 tcggtgacga cgtaggtcat gcgggagaga gcctgcggcg atgaaaaggt taattgaatc
     3061 cgtagtttag catgcgcgct tgggttccgg gacgcgcgcc ttcataatga aggctccctg
     3121 caataccccc cgaggagcca tgagcagcag cacgcagatt catcacgtta cggacgcaag
     3181 cttcgagccg gaagtcctcc aatccgaggt gccggtgctg gtggactact gggccgaatg
     3241 gtgcggcccc tgcaagtcga tcgcgccaat cctcgaccag gtggcccagg agtacgacgg
     3301 gcgcctgaag atcgccaagc tgaacgtcga cgagaaccag gcggtgccgg caaaatttgg
     3361 cattcgcggc atcccgacgc tgatgctgtt caagaacggc accctcgagg cgacgcgcgt
     3421 cggcgcgctg tcgaagtcgc agctcaccgc gtttcttgac agcaatatct aaaccactta
     3481 cgctgcgcag caatcccgcc ccggcgatcc ggggcggctg aagctaagaa ggtgcagtaa
     3541 gaaccggcgc acgccgcagt tcccaggcgc agcgccctcc ccatacggaa gaagtcggca
     3601 ggtccccgca gcctctattc actcgtccat gcatctctcc gaactgaaga gccagcacgt
     3661 cacgcaactc gtggaaaccg cggtcgcgaa ccagatcgac aacgcgagcc gcatgcgcaa
     3721 gcaggacctg atcttcgcgc tgctgaagaa ccaggcgaaa aaaggcgagt cgatcttcgg
     3781 cgacggcacg ctggaagtgc tgcccgacgg cttcggcttc ctgcgctcgc ccgagacctc
     3841 gtatctggcg agcaccgacg acatctacgt cagcccgtcg cagatccggc gcttcaacct
     3901 gcacaccggc gactccatcg agggcgagat ccggacgccc aaggacggcg agcgctactt
     3961 cgccctggtc aaggtcgaca aggtcaacag cgaggtcccc gaggccgcca agaacaagat
     4021 cctgttcgag aacctcaccc cgctccatcc ggacgagatg ttcaagctgg agcgcgagat
     4081 caaggccgag gagaacctca ccgggcgcgt catcgacatc gtcgcgccga tcggtaaggg
     4141 ccagcgcggc ctcatcgtct cgccgccgaa ggcgggcaag accgtgatgc tgcagcacat
     4201 cgcccacgcg ctcgtcgcca accatcccga agtgagcctc atcgtgctgc tgatcgacga
     4261 gcggcccgag gaagtgaccg agatgagccg gtccgtccgc ggcgaagtcg tggcatccac
     4321 cttcgacgag ccggcctcgc gccacgtgca agtcgccgag atggtgatcg agaaagccaa
     4381 gcgcctggtc gagcacaaga aggacgtcgt catcctgctc gactccatca cgcgcctcgc
     4441 gcgcgcctat aacaccgtgg tgccggcctc gggcaaggtg ctcaccggcg gcgtagacgc
     4501 caacgccctg caaaagccca agcgcttctt cggcgccgca cgccacgtcg aggaaggcgg
     4561 ctcgctcacc atcatcgcca ccgcgctcat cgacaccggc agccgcatgg acgacgtgat
     4621 ctacgaggaa ttcaagggca ccggcaacat ggagatccat ctcgaccggc gcatgcacga
     4681 aaagcgcatc ttccccgcga tcaacgtcaa ccgctcgggc acgcgccgcg aggagctgct
     4741 gctgcagaag gacgtgctgc agaagatctg ggtactgcgc aagctgctct tcccgatgga
     4801 cgagctcgag gcggccgaat tcctcatcga caagctgcgc gccaccaaga acaacgcaga
     4861 gttcttcgac tcgatgcggc gagggtaaac agggccccgg caagcgatga cgccagcgtc
     4921 atcgcgacgc gtttgcagtt caatacatct cgcctttttc tggccggaag attcacccgg
     4981 ccagaaaaag gcgagaccat agaaacacca tcgtaggcac cgcgtaggca ccgcctcagc
     5041 ccccgcgcta cactcccccc gtttcctaag gggggaatca tcctcgacac cacgcgccgg
     5101 gtcgcgacgc ccgaaggcat agagctcacc ctgcagctcg tcgggccagt gccgcgcgcg
     5161 ctcgcctggg ccatcgacct cgtgctgcgc gcgctggtgg tgatcggcgt ggtcatggtc
     5221 gcgggctatt tcggcagggc gggctggggc ctcgcgctgc tcgtcatgtt cgtcgtcgag
     5281 tggctggcgc ccgcctggtt cgaggtggtc tggaacggac agacgccggg caagcgcgcg
     5341 ctcggcatcg cggtgctgaa cgacgacggc acgccggtgc gctggagcgc gtcgatgacg
     5401 cgaaacctgc tgcgcgcggt ggacttcctg ccgttcctgt acggcttcgg cctcatcacc
     5461 atgctcgcca gccgcgattt caagcgcctg ggcgacctcg ccgccggcac ggtggtggtg
     5521 tacgcgccgg gcaaggcgcg cgcgcgcacg ctgcccgagg cgccggccga gccgccgccc
     5581 tttccgctct cgctcgagga gcagcgcgtg gtgctcgacc ttgccgagcg ctcggcgagc
     5641 ctcacgcgcg agcggctgga ggagctcgcc tcgctgcccg tgccgctcac cggcggtgcg
     5701 agcggcgcca cggcggccgc gcgcctcctt ggtatggcga gccatctctc gggccggccg
     5761 tgaagcaggc gcccttcgag gccgcgcacg gtgaagagtg gcgcgcgttc gagcgcttcc
     5821 tcgacgctgc cgcgggcgcg ccgccgccct ttgcgcccga ggaaatgccg cgccgctacc
     5881 ggcgcctgtg ccaccatctc gcgctcgccg ccgaccgcca gtacagcccc gagctcgtcg
     5941 accggctgaa cctgctcgcg ctgcgcggcc accacgcgct ctacgccaac cgccgccgcc
     6001 agtcgcagcg catcgtcgac ttcctgctgg tcgccttccc ttcgctggtg cgcgccgagt
     6061 ggcgcgtggt catcgcggcg gcgctgcttt tcttcggccc gctcgtcggc ctgatcgcgg
     6121 tgctgcagct ctatcccgag ttcgtgcatt acctgctcga tccgcggcag atcgcgggct
     6181 ttcacgacat gtacgacccg gcgaaccggc gcctcggcat gcgcgaagcc gatacccacc
     6241 tcgcgatgtt cggctactac atctggaaca acgtgcgcat cggcttccag accttcgccg
     6301 gcgggctcct cgccggcctg ggcacgctgt ggtacctggc ggcgaacggc gtgatcctcg
     6361 gcgcggtcgc gggctacctg acgcagatcg gcttcggcga gactttctgg tcgttcgtct
     6421 cggggcacgc cgcgctcgag ctcaccgcga tcgtcatctc cggcgcggcc gggctgcgcc
     6481 tcgggatggc ggtgatcgcg cccggcaacc gctcgcgcaa ggccgcgctg gtggcggtgg
     6541 cgcggcccgc cgtgcgcctg atgtacggcg cggcgctgat gttcacggcg gccgccttcg
     6601 tcgaggcgtt ctggtcgccg ctcacctcgg tgcccaccgc ggtgaagatc tgggccgggc
     6661 tcgccggctg ggcgctgctg ctcgcctacc tcgtgctcgc gggacgcgca cgtgcagctc
     6721 gataggctcg ccatcgcgct tcggccgcgc tcggcctggg aatcggtcga cctcggcgtc
     6781 ggcatgctgc gcgagtggtg gcgaccgctc tatgcgtcgt tcgccgcggt gtacctgccg
     6841 ctcgccgcgg catgcttctg ggcgggctgg tccaccggca aggcgtggct ggcgatggta
     6901 atgctgtggt ggctgaagcc gctcttcgac cgcgtggcgc tgcacgtcgt ctcgcgcgca
     6961 gtgttcggcg acgtgctcgg cgtgcgcgcg accctcgccc aggcgcgcgc ctggctcgcg
     7021 agcgggctct tcggcgcgct cacgcttggg cgcgtcgaca ctgcgcgctc gttccacctg
     7081 ccggtgaagc agctcgaggg ccagggctgg cgcgcggcgc gcgcacggcg cgcgctgctc
     7141 gggcgccgca cccacggcta cgccgtgtgg ctgacgctcg cctgcgtcaa cttcgagctg
     7201 gtgtttgtat ggtcggccga catgctgtgg aacatcgtcg tgccggcgaa ggcgcaggag
     7261 ggcacgagca tctgggaggc gatgttcgcc gacgccgcgt tcgataacgt gttcagcctc
     7321 gccgatttcg ccgcctatgc gatggcggtg ttcatcatcg agccgctgta cgtcgccgcc
     7381 gggttcgcgc tgtacctcaa ccggcgcacg ctgctcgagg gctgggacct ggaagtggcg
     7441 ctgcggcgca tcggcgcgca gcgcgcggcc gtcgccgcga cgctgctgct cgccgcggcg
     7501 ctcgccctgc tcgcgcccgc cgcgcccgcg ctggcgcagg cgccggccaa ggatccgaag
     7561 cgcgagatcg ccgaggtgct gaaagcgccc gagttccccc accgccgcac cgtgacgcgc
     7621 tggcagcggc gcagcagcga tgaagcgcac gcggggcccg gctgggacct gagctggctg
     7681 cgcgcaatcg gctacgcatt cgccaagtcc tcggaggtcg cgctgtggat cgccgcgggc
     7741 acgctcgtcg ccctcggact gtggtgggcg ctgcgcctca tgccgcgcgc cccgcccgcg
     7801 acgcgcgcgc cctaccagcc gcccgccgcg ctgttcggca tggcgctcgc ccccgagacg
     7861 ctgcccgcgg acctcgccgg cgcggtgcgc gcgcacctcg ccgcgggccg cgtgcgcgag
     7921 gcgctggcac tgctgtatcg cggcacgctc tcggcgctgg tgcacggccg cggcatcgag
     7981 ctgagggcga gcgacaccga gggcgaggcg ctcgcgcgcg tgcgcgcgca gggcgaggcc
     8041 gccaccgcgc agttcttcgg cgagctggtg ctgcactggc agcgcgcggc ttacgcgcgc
     8101 cgcccgcccg ccgccgccga ggtcgagcgg ctgctcgccg cctatggcga gcgcttggca
     8161 ccgcggagcc cggcatgagc cggccgctgc gcctcgcgct cggcgggctg ctcctcgtgc
     8221 tgctcgcctg gtacctgtcg ggcttggaaa aggtgaccgt cacgcagcgc gtcggcttct
     8281 cgggcgaggc acggctgcgg ccgttcctcg ccgccgagcg cttcgccgag cgcatggggc
     8341 tcgccgcgcg cgagctgcgc gcgctgcccg agctcgagca gctgcccccg aacggcgtgc
     8401 tcgtcctgcc gcgcaaccgc cagtcgatcg aagcgcggcg cgccgcgcag ctcgtgctct
     8461 gggcggcggc gggcaaccac ctcatcgtcg aggccgagcg cgacggcatc gccgacccgc
     8521 tgctcgctca actgcgcatc acgcgcgccg atgcgcaggt gcgctcgccg cggctcacgg
     8581 ccggtgttcc cggcaacgag cgcgagctga agctctcggg ctttgccgcc ggccggctgc
     8641 aggccgaagg gcgcgcgccg cgctatcgcg tcggcccggc gggcgaggcg cggctcatca
     8701 cggtggcgca cggccagggg ctggtgagcg cggcggtgga cctgaacttc gcgcgcaacg
     8761 cctggatcgg cgagcacgac aatgcggtgc tgctgtggga gctgctcagc ctgcacccgg
     8821 ccggagaact gctcgtattc caccacccgc ggcggctctc gctgtgggat ttcctcgtcg
     8881 cgcgcgcacc ctgggcgctg ctcgcggccg cggccctcgt cgcgctgtgg ctgtggcgca
     8941 tcgcgccgcg ctacggcacc gtgctgcccg acgcgccgcc cgcgcgccgg cgcctgctcg
     9001 atcacctgcg cgccgccggg cgcttcctgt ggtcgcacgg gctgcgcgcg cctctggcgg
     9061 ccgcagcgcg ggaggcggcg ctgcgccgcg tcgcgcgcgc ctatcccgat ttcgcccacg
     9121 cgccgcgcgc cgagcaggcc gcgcgcctgg ctgccctggc cggcctggcg ccggccgacg
     9181 ccgcgctgct gcttgccgcg cccggcgcgg cgccggcgcc gggcggcgcc gaattcatgc
//
LOCUS       DBNHKE010000099        12421 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1338801, whole genome shotgun sequence.
ACCESSION   DBNHKE010000099 DBNHKE010000000
VERSION     DBNHKE010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1338801"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..603)
                     /locus_tag="AC16O9_06115"
     CDS             complement(184..603)
                     /locus_tag="AC16O9_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516225.1"
                     /translation="MAAEDERQQRGSDPKLDPAALYLEEVFTDRRIGTIRRMTPVKAD
                     ATTDLSRPVVYVGETQVLTQVGTLPIAFEIPAKTLEDAAKQFGPLAKEAIERTMKELQ
                     ELRRQAASSIVVPQGGMPPAGGLGGLGGAGGGKIQLP"
     gene            complement(658..2208)
                     /locus_tag="AC16O9_06120"
     CDS             complement(658..2208)
                     /locus_tag="AC16O9_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIR6516226.1"
                     /translation="MELSRADHSASPPRIEVPREYNAAYDLVERNLAAGRGGKAAFHD
                     DAGTLTYAQLGERANRFGSGLLAMGLRMEDRVVLAMHDTNDWPVAFLGAIRAGIVPVA
                     VNTLLTTKDYEYMLSDSRAKALVVSEALLPQFKPVVDRLPFLKHVIVAGASFNKVLEK
                     GATKLDPAPTTRDDACFWLYSSGSTGMPKGTVHAHSSMIETAELYARGVLGMKESDVV
                     FSAAKLFFAYGLGNSLSFTLAAGATSVLMAERPTPAAVFKRLTERKPTLFYGVPTLYA
                     AMLADAAFPKKEALALRLCVSAGEALPPQIAKSFKEKTGLDILDGIGSTEMLHIFLSN
                     RPGDLRYGTTGKAVPGYELRLVDEEGKPVKQGELGELEIRGTTSAMYYWNNRAKSRAT
                     FVGAWTKSGDKYTLDADGFYAYGGRTDDMLKVSGIWVSPAEVEAALVGHPAVLEAAVV
                     GKEDEQKLVKPKAYVVLKPGSKASEDELKAHVKEKLAPYKYPRWIEFANELPKTATGK
                     IQRFKLRA"
     gene            complement(2234..2707)
                     /locus_tag="AC16O9_06125"
     CDS             complement(2234..2707)
                     /locus_tag="AC16O9_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR6516227.1"
                     /translation="MKINILVGTMTGTAQMCAQEMELALDGDDLQVVTHLMDKLEPSV
                     FADREAVYLVCTSTYGQGDVPDNAKAFYEALGAQKPGLAGVRYGVFGLGDRTYAETFN
                     FGGKRFDELLASLGAERIGERYMHDASSGTLPEEVALEWAAAWVQQVRERLAQSA"
     gene            2760..3545
                     /locus_tag="AC16O9_06130"
     CDS             2760..3545
                     /locus_tag="AC16O9_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6516228.1"
                     /translation="MGAPLLPLHRDDQAETAYLARLGERVRAWRTEHAMTRKALALAS
                     GVSERYLAQLEGGQGNISVLLLRKLAHAMQVPVELLVREHNAPARAGRVALLGLRGAG
                     KSTLGARLAQALQLPFIELDREVEKEAGAPLAEVFSMYGQEAFRRFERKALARVLKQL
                     PRAVIATGGSLVTDPDAYGLLLDHCTCIWLKATPEEHMARVMAQGDVRPFKGSPAALE
                     EIRGLLADRDRLYARADAVLNTSGRAPKQSLAELKALVAKETA"
     gene            3542..5140
                     /gene="boxC"
                     /locus_tag="AC16O9_06135"
     CDS             3542..5140
                     /gene="boxC"
                     /locus_tag="AC16O9_06135"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HIR6516229.1"
                     /translation="MNAPGTPPVRVSFETQPDAYHHWKLAFDGPVATLSLDINEDKGI
                     APGYKLKLNSYDLGVDIELHDALNRIRFEHPEVKTVVLTSGKSRMFCSGANIYMLGLS
                     SHAWKVNFCKFTNETRNGLEDSSAHSGLKFLAALNGTTAGGGYELALACDEIAMVDDR
                     SSTVSLPEVPLLGVLPGTGGLTRIIDKRKVRRDLADVFCTTAEGVRADRAQQWKLVDH
                     IAKPQEFQDAVRARALELARGSDRPGGEGVKLTPLGANRLVTVELDAAGGTATLTVRG
                     PAEVTLERGADWYPLRLARELDRAILELRHNQLEIGLWLLRTEGDPARVLELDALLEN
                     NASDWFVRETVGMLRRTLARLDVTSRSMFAVIDKGSCFAGTLAELALAADRSFMLDAE
                     DGGPSVALSNLNFGAYPMVNGDSRLSAHYCGQVPALERGELLDAKAAEQAGLVTAAPD
                     ELDWDDEVRIAVEERVSLSPDALTGMEASLRFTGRENMLTRVFGRLSAWQNWIFIRPN
                     AVGEQGALKVYGTGAKAKFNKERV"
     gene            5140..6555
                     /gene="boxB"
                     /locus_tag="AC16O9_06140"
     CDS             5140..6555
                     /gene="boxB"
                     /locus_tag="AC16O9_06140"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HIR6516230.1"
                     /translation="MAAINYTDKIPNNVNLANDRALQRALEHWQPHFLDWWKGMGPTD
                     FQNSEVYLRTATSVDADGWATYGAVQMPNYRWGIFLADPVAERKIGFGDNMGQDAWQQ
                     VPGEFRSTLRRLIVTQGDTEPASVEQQRLLGHTCPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHAYFGRDGREEAEELLARHSGDADKPRILKTFNEPISDWLSFFCFTYFTDRDGKY
                     QLKSLAESAFDPLSRTCRFMLTEEAHHMFVGETGIGRVVKRTLEVMKELGTDDPAKIR
                     AAGAVDLPLLQKYLNFWCSSSLDLFGSEISSNSASNFANGLKGRPDESQYEDHVLRAA
                     QFKLETPKGTQDVPMLNALNEVMRESYLRDCEIGLKRWNRAIERAGHAFRLALPSPHF
                     RRSIGAWVDAPVTPQGQMISREEYDKRVHEWIPSEQDRAFVRSLMQRVVEPGKMAGWV
                     APPERGINNLPVEYEYVKLAA"
     gene            complement(6552..7445)
                     /locus_tag="AC16O9_06145"
     CDS             complement(6552..7445)
                     /locus_tag="AC16O9_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6516231.1"
                     /translation="MTAILSPRVGIPLLMLLSSIFAANHIAARLAFDHGASVAAAVAV
                     RSGITALVMVVLLHLRGISMALPRATLTRGLGIGALVAVQSYCLYSAVAVIPVALALL
                     AFNTFPLLFVLATWAAGGEHPGRRAFVAMPLALFGLVLALDILGTLETMAGRWAEIGV
                     GVGWAFGAAASFAMVLFFTTRHLKDVDGRLRTLLTMGTTAVVIGLGGAAAGALALPAD
                     ATGWLGLALLTVCYGTAITAIFTVVPRLASPSNTAALNFEPIAALGLGFVILGQGVAP
                     RQLVGAFIVVGAVVLIGAKKH"
     gene            7464..8042
                     /locus_tag="AC16O9_06150"
     CDS             7464..8042
                     /locus_tag="AC16O9_06150"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR6516232.1"
                     /translation="MSNLARLRGFIRDMTRLTERHGADEQRMLDEGEKLLRGLIAQDD
                     WLPEDYARASPAGYRQYLLHCDPMERFSVVSFVWMPGQKTPIHDHTVWGLVGVMRGEE
                     ICEEYTREVKPAGRHPVRRGQVDRVSPRIGDIHVVANEGSEVAVSIHVYGANIGAVRR
                     HTYDAANGARHDFVSGYANASIPNLWDRSREA"
     gene            8018..8374
                     /locus_tag="AC16O9_06155"
     CDS             8018..8374
                     /locus_tag="AC16O9_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516233.1"
                     /translation="MGPLQGSVAAALLAAAFVAQACEVPGDGTSLRRALLKVKYLPET
                     EAWERAARKSATVQFVLSLGAPHKVDGRCYWPIEARSDGALWNTFYATPSGEHILVGT
                     PGSGLVPLDTWRNAAR"
     gene            complement(8408..8803)
                     /locus_tag="AC16O9_06160"
     CDS             complement(8408..8803)
                     /locus_tag="AC16O9_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6516234.1"
                     /translation="MSLVTLIEYDAAGAEVRAVYDDIMKTRGVDWINNFWKALAHDPA
                     QLRRTWEQVKQVMAPGALDPLVKEMIYVAVSATNGCEYCTYSHTASARKRGMSDAMLM
                     ELMAVVGLANQTNRLANGLRVDVDERFKP"
     gene            8839..9291
                     /locus_tag="AC16O9_06165"
     CDS             8839..9291
                     /locus_tag="AC16O9_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6516235.1"
                     /translation="MLLKGSCHCGAVRFSLESETPYPYMHCYCSICRKTAGGGGYAIN
                     IMGQAASFKKRGRTRIYRATRGGGERHFCPRCGSALWVWDKRWPEGIYPFASAIDTRL
                     PAPKERQHILLGSKASWVPAPRGRHDKHFREYPREAIIDWHRKRGLLR"
     gene            9295..9978
                     /locus_tag="AC16O9_06170"
     CDS             9295..9978
                     /locus_tag="AC16O9_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516236.1"
                     /translation="MPGWISALSPETLLVAIATMGGIVALLLTVAAVRRLRAWEIRRG
                     SVYLFLAAVVAALGVGGAMLAASLHTYARLTHEQEAARAVLRALGPQRFELLLVREGQ
                     LSQRFELRGDEWQIDARVLKWRGMGALLGFDTVYRFERLSGRYADNDAERKAPRSVHS
                     LSPDGTVDFWQLLKKYHGYMPLADALYGSAAYVPMADGAQYVVSVSATGLLIRPLNDT
                     AKQAVGGWK"
     gene            9975..11450
                     /locus_tag="AC16O9_06175"
     CDS             9975..11450
                     /locus_tag="AC16O9_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR6516237.1"
                     /translation="MKRFAALLALALLAGCAGSGAYFSDAGPQPAREPRALAAWPWQE
                     VWTGVVFQGRKVGFARLALRPAATPGRWEIETESALQLRFLGVDKKVGLRALDEVRAD
                     LALERFRYEYHLDGSRLAVAGERDGDALVVRTDSSGARREQRLSLDAPTVPASALALL
                     PAQRGLNVGERARVAVFVGESQSLEQAELRALAHERSPLFEGVALRVATTLLGLETEA
                     WYDAEARPLLETALHGAMLSALEEPARARAYLVEASLNKDESLLEFSLLRSPPVREAR
                     RTAALTLSLEGVPAALDVPGTAGQRCVRDGARLECRVDRRTARSAGDASEAASYLRPS
                     LAVPSNERRFLELAQLIVAGALDAEARIERLLAWMDGNIAKAALDAFSAADVLRERRA
                     ECQGHAYLFAAFARALGLPTRVVNGVVYSAEHGGFLYHTWNEVWLEGAGWRAVDPTFG
                     QSHADATHVALAVGETPAAIAPLAAMVGRARVAAIGEVAHW"
     gene            11466..12011
                     /locus_tag="AC16O9_06180"
     CDS             11466..12011
                     /locus_tag="AC16O9_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HIR6516238.1"
                     /translation="MKFIFDLFPVILFVATYVWTDNIYIATAVIIPASIAQVAYVWFR
                     YRRIERMLLVSMVLVLILGGLTLFLQDERFIKWKPTVLYWLFAAVLALSPLVAKKNIV
                     RLMMEKGFTAPERIWNSLNVAWVLFFVFMGVLNLYIAQNFSLGFWVQFKLWGLTGLMF
                     AFIAAQIAVLYKYVDEPEAKK"
     gene            12008..12286
                     /locus_tag="AC16O9_06185"
     CDS             12008..12286
                     /locus_tag="AC16O9_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR6516239.1"
                     /translation="MSTADDIRARLAVLQPESLELEDESERHRGHAGYRPGGGTHWRL
                     SIVARAFAGQSTVARHRLVYQALGGLMQDPIHALAIHARAPGEPGAGA"
BASE COUNT         1876 a         4409 c         4247 g         1889 t
ORIGIN      
        1 gcgcggccgc cggctcgggc gccgcgcgca gctcgcagct cggcgcgcag cagcgctacg
       61 accacgcctt ccagcagtgc atgtacgcga agggacacaa ggtcccggtg gaaggctacg
      121 cgctgccgca gcgcagcccg cggcgcgcga gcattccgcc gccgccgccg ccgcgctgac
      181 gccctagggc agctggatct tgccgccgcc cgcgccgccc aggccgccca ggccgccggc
      241 gggcggcatg ccgccttgcg gcaccacgat cgacgacgcc gcctggcgcc gcagctcctg
      301 cagctccttc atggtgcgct cgatcgcctc cttggcgagc gggccgaact gcttggccgc
      361 gtcctccagc gtcttggccg gaatttcgaa cgcgatgggc agcgtgccga cctgcgtcag
      421 cacctgcgtc tcgcccacgt agaccacggg gcgcgagagg tcagtggtgg cgtcggcctt
      481 gaccggcgtc atgcggcgga tggtgccgat gcgccggtcg gtaaacactt cctcgagata
      541 cagcgccgcg gggtccagtt tcggatcgct gccgcgctgt tgcctttcgt cttctgccgc
      601 catgagcgtc cctttagatt ggcctaaggg cgctagttta ctgcgcgcgg cccgtgctca
      661 cgcgcgcagc ttgaagcgct ggatcttccc ggtggccgtc ttcggcagct cgttggcgaa
      721 ctcgatccag cgcggatact tgtacggcgc gagcttttcc ttcacgtgcg ccttcagctc
      781 gtcctcgctc gccttcgacc cgggcttgag caccacgtag gccttgggct tcaccagctt
      841 ttgctcgtct tccttgccga ccaccgccgc ctcgagcacc gccgggtgcc cgaccagcgc
      901 ggcctcgact tccgccggcg acacccagat gcccgatacc ttgagcatgt cgtcggtacg
      961 cccgccgtag gcgtagaagc cgtcggcgtc cagcgtgtac ttgtcgccgc tcttggtcca
     1021 cgcgccgacg aatgttgcgc ggctcttggc gcggttgttc cagtaataca tggcgctggt
     1081 cgtcccgcgg atctccagct cgccgagctc gccctgtttc accggcttgc cctcctcgtc
     1141 caccagccgc agctcgtagc cgggcaccgc cttgccggtg gtgccgtaac gcaggtcgcc
     1201 cgggcggttg gagaggaaga tgtgcagcat ctcggtggag ccgatgccgt cgaggatgtc
     1261 gagcccggtc ttttccttga agctcttcgc gatctgcggc ggcagcgcct cgcccgcgga
     1321 cacgcacagg cgcagcgcga gcgcctcctt cttcggaaag gcggcgtcgg cgagcatcgc
     1381 cgcgtagagc gtcggcacgc cgtagaacag cgtcggcttt ctctcggtga ggcgcttgaa
     1441 caccgcggcc ggcgtcgggc gctcggccat cagcaccgag gtcgcgccgg ccgccagcgt
     1501 gaaggacagc gaattgccca ggccgtaggc gaaaaacagc ttcgccgccg agaacacgac
     1561 gtcggactcc ttcatgccga gcacgccgcg cgcatacagc tcggccgtct cgatcatgct
     1621 cgagtgcgcg tgcacggtgc ccttgggcat gccggtcgag cccgaggagt acagccagaa
     1681 gcaggcgtcg tcgcgggtcg tcggcgccgg gtcgagcttc gtcgcgccct tctccagcac
     1741 cttgttgaac gaggcgccgg cgacgatgac gtgcttgagg aacggcagcc ggtccaccac
     1801 cggcttgaac tgcggcagca gcgcttccga gacgacgagc gccttggcgc ggctgtcgga
     1861 cagcatgtac tcgtagtcct tggtcgtgag cagcgtgttc accgccaccg gcacgatgcc
     1921 ggcgcggatg gcgccgagaa acgccaccgg ccagtcgttg gtgtcgtgca tcgcgagcac
     1981 gacgcgatcc tccatgcgca gccccatggc gagcaggccg ctgccgaagc ggttggcgcg
     2041 ctcgccgagc tgcgcgtagg tcagcgtgcc ggcgtcgtcg tggaacgcgg ccttgccgcc
     2101 gcgcccggcc gcgaggttgc gctcgaccag gtcgtaggcg gcgttgtatt cgcgcggcac
     2161 ctcgatgcgt ggtggcgatg ccgaatggtc ggcccgggac agttccatgc gctttgcctc
     2221 ctccgctgca attctaggcc gactgcgcga gcctttcgcg cacctgctgc acccaggccg
     2281 cggcccactc cagcgccacc tcttcgggca gcgtgccgct cgaggcgtcg tgcatgtagc
     2341 gctcgccgat gcgctccgcg ccgaggcttg cgagcagctc gtcgaagcgc ttgccgccga
     2401 aattgaaggt ctcggcgtag gtgcggtcgc cgaggccgaa caccccgtag cgcacgccgg
     2461 cgaggccggg cttctgcgcg ccgagcgcct cgtagaacgc cttggcgttg tcgggcacgt
     2521 cgccctggcc gtaggtcgag gtgcacacca ggtacaccgc ctcgcggtcg gcgaagaccg
     2581 acggctcgag cttgtccatc agatgcgtca ccacctggag gtcgtcgccg tcgagggcga
     2641 gctccatttc ctgcgcgcac atctgcgccg tgccggtcat cgtgccgacg aggatgttga
     2701 ttttcatgcc ccacacagta taatgcagaa tttcacccac gtaaagcact atattgcata
     2761 tgggcgcccc ccttctgccg ctgcatcgcg acgaccaggc tgaaaccgcg tatctggcgc
     2821 ggctcggaga gcgcgtgcgc gcctggcgca ccgagcacgc catgacgcgc aaggcgctcg
     2881 ccctcgcctc gggcgtctcg gagcgctacc tggcgcagct cgagggcggc cagggcaaca
     2941 tctcggtgct gctgctccgg aagctcgcgc atgccatgca ggtgccggtg gagctgctgg
     3001 tgcgggagca caacgcgccc gcgcgagccg gccgcgtcgc gctgctcggc ctgcgcggcg
     3061 ccggcaagtc gacgctcggc gcgaggctgg cgcaggcgct gcagctgccg ttcatcgagc
     3121 tcgaccgcga agtggagaag gaagcgggcg cgcccctcgc cgaagtgttc tcgatgtacg
     3181 gccaggaagc gttccgccgc ttcgagagaa aggcgctggc gcgggtgctg aagcagctgc
     3241 cgcgcgccgt gatcgccacc ggcggcagcc tggtcaccga tcccgacgcc tacggcctgc
     3301 tgctcgatca ctgcacctgc atctggctga aggccacgcc cgaggagcac atggcgcgcg
     3361 tcatggcgca aggcgacgtg cgccccttca agggcagccc ggccgcgctc gaagagatcc
     3421 gcgggctgct cgccgaccgc gaccggctgt acgcgcgcgc cgacgccgtg ctcaatacct
     3481 ccggccgggc gcccaagcaa agcctcgccg agttgaaggc gctcgtcgcc aaggagaccg
     3541 catgaacgcc cccggcaccc cgcccgttcg tgtgagcttc gagacgcagc ccgacgcgta
     3601 tcaccactgg aagctcgcgt tcgacggccc ggtggccacg ctcagcctcg acatcaacga
     3661 ggacaagggc atcgcgccgg gctacaagct gaagctcaat tcctacgacc tcggcgtcga
     3721 catcgagctg cacgatgcgc tgaaccgcat ccgcttcgag catcccgagg tgaagacggt
     3781 ggtcctcacc agcggcaaga gccgcatgtt ctgctcgggc gccaacatct acatgctcgg
     3841 cctctcgagc cacgcctgga aggtgaactt ctgcaagttc accaatgaga cgcgcaacgg
     3901 cctggaggat tcgagcgcgc attccggatt gaaattcctc gccgccctga acggcaccac
     3961 cgcgggcggc ggctacgagc tcgcgctcgc ctgcgacgag atcgcgatgg tcgacgaccg
     4021 ctcgagcacc gtgagcctgc cggaagtgcc gctgctcggc gtgctgccgg gcaccggcgg
     4081 cctcacgcgc atcatcgaca agcgcaaagt ccggcgcgac ctcgccgacg tcttctgcac
     4141 caccgccgag ggcgtgcgcg ccgaccgcgc gcagcagtgg aagctcgtcg atcacatcgc
     4201 caagccgcag gaattccagg acgcggtcag ggcgcgcgcg ctcgagctcg cccggggctc
     4261 cgaccgcccc ggcggcgagg gcgtgaagct gaccccgctc ggtgcgaacc ggctcgtcac
     4321 agtcgagctc gacgccgccg gcggcacggc caccctcacg gtgcgcggtc ccgccgaggt
     4381 gacgctggag cgcggcgccg actggtaccc cttgcgcctc gcgcgcgagc tggaccgcgc
     4441 catcctcgag ttgcgccaca accagctcga gatcggcctg tggctcctga ggaccgaggg
     4501 cgacccggca cgcgtgctcg agctggacgc cctgctggag aacaacgcca gcgactggtt
     4561 cgtgcgcgag accgtgggca tgctgcgccg gacgctggcg cggctcgacg tgacgtcgcg
     4621 ctcgatgttc gcggtcatcg acaagggctc gtgcttcgcc gggacgctcg ccgagctcgc
     4681 gctcgccgcg gaccgcagct tcatgctcga cgccgaggac ggcgggccga gcgttgcgct
     4741 gagcaacctg aacttcggcg cctacccgat ggtgaacggg gattcgcgcc tgtctgcgca
     4801 ctactgcggc caggtaccgg cgctcgagcg cggcgagttg ctcgacgcca aggcggcgga
     4861 acaggcgggc ctggtcaccg ccgcgcccga cgagctcgac tgggacgacg aggtgcgcat
     4921 cgcggtcgag gagcgcgtga gcctgtcgcc cgatgccctc accggcatgg aagcgagcct
     4981 gcgcttcacg ggccgggaga acatgctgac gcgcgtcttc gggcgcctgt cggcctggca
     5041 gaactggatc ttcatccggc ccaacgcggt cggcgaacag ggtgcgctca aggtgtacgg
     5101 caccggcgcc aaagccaaat tcaacaagga gcgggtctga tggccgccat caactacacc
     5161 gacaagatcc ccaacaacgt caacctggcg aacgaccgcg cgctgcagcg cgcgctcgag
     5221 cactggcagc cgcactttct cgactggtgg aagggcatgg gccccaccga tttccagaac
     5281 tcggaggtct atctgcgcac cgccacgtcc gtcgacgccg acggctgggc cacctacggc
     5341 gcggtgcaga tgcctaacta tcgctggggc atcttcctcg ccgacccggt ggccgagcgc
     5401 aagatcggct tcggcgacaa catgggccag gacgcctggc agcaggtgcc gggcgagttc
     5461 cgctcgacgc tgcgccggct catcgtcacg cagggcgaca ccgagcccgc gtcggtggag
     5521 cagcagcgcc tgctcggcca cacctgccct tccctctacg acctgcgcaa cctgttccag
     5581 gtgaacgtgg aggaaggccg gcacctctgg gcgatggtgt acctgctgca cgcatatttc
     5641 ggccgcgacg gccgcgagga agccgaggag ctgctcgcgc gtcactccgg cgacgccgac
     5701 aagccgcgca tcctcaagac cttcaacgag ccgatctcgg actggttgtc gttcttctgc
     5761 ttcacctact tcaccgaccg cgacggcaag taccagctga agagcctggc cgagtcggcc
     5821 ttcgacccgc tgtcgcgcac ctgccgcttc atgctcaccg aggaagcgca ccacatgttc
     5881 gtcggcgaga ccggcatcgg ccgcgtcgtg aagcgcaccc tggaggtgat gaaggagctc
     5941 ggcaccgacg atccggcgaa aatccgcgcc gcgggcgcgg tcgacctgcc gctgctgcag
     6001 aagtacctga acttctggtg ctcgagctcg ctcgacctgt tcggctcgga gatctcctcg
     6061 aactcggcat cgaacttcgc caacggcctc aagggccgcc ccgacgagtc gcagtacgag
     6121 gaccacgtgc tgcgcgccgc gcagttcaag ctcgagacgc cgaaaggcac gcaggacgtt
     6181 ccgatgctga acgcgctgaa cgaagtgatg cgcgagagct acctccggga ttgcgagatc
     6241 gggctgaagc gctggaaccg cgccatcgag cgcgccggcc atgcgttccg gctcgcgctg
     6301 ccctcgccgc atttccgccg ctccatcggt gcctgggtcg atgcgccggt gacgccccag
     6361 ggacagatga tttcgcgcga ggagtacgac aagcgcgtgc acgagtggat cccgtccgag
     6421 caggaccggg cgttcgtgcg ttcgctcatg cagcgcgtgg tcgagccggg caagatggcc
     6481 ggctgggtgg cgccgcccga gcgcggcatc aacaacctgc cggtcgagta cgagtacgtg
     6541 aagctcgcgg cctagtgctt cttggcgccg atcaggacca cggcgccgac gacgatgaac
     6601 gcgccgacca gctggcgcgg cgcgacgccc tggccaagga tcacgaagcc caggccgagg
     6661 gcggcgatcg gctcgaaatt cagcgctgcg gtgttgctgg gcgaggcgag ccgcggcacc
     6721 acggtaaaga tcgccgtgat cgcggtgccg tagcacaccg tcagcagcgc cagtcccagc
     6781 cacccggtcg cgtccgccgg cagcgccagc gctccggccg ccgcgccgcc caggccgatg
     6841 acgacggcgg tggtgcccat ggtgagcagc gtgcgcaggc gcccgtccac atccttcagg
     6901 tggcgtgtgg tgaagaacag caccatcgcg aacgaggccg ccgcgccgaa cgcccagccc
     6961 acccccacgc cgatctccgc ccagcgcccg gccatcgttt ccagggtgcc gaggatgtcg
     7021 agcgcgagga cgaggccgaa cagggcgagc ggcatggcga cgaacgcgcg ccgccccggg
     7081 tgctcgcccc ccgcggccca ggtggccagc acgaagagca gcgggaacgt gttgaaggcg
     7141 agcaacgcca gcgccaccgg aatgacggcg accgccgaat acaggcagta gctctgcacc
     7201 gccaccagag cgccgatgcc cagcccgcgg gtgagtgtcg cgcgcggcag cgccatggag
     7261 atgccccgca ggtgcagcag caccaccatc accagcgcgg tgatgccgga gcgcacggcg
     7321 accgccgccg cgacgcttgc gccgtggtcg aaggccagcc gcgcggcgat gtggttggcg
     7381 gcgaagatgc tggagagcag catgagcagc gggatgccga cccgcggcga tagaatcgcg
     7441 gtcatggttc gggagcttaa ggcatgagca acctcgcgcg cttgcgcggt ttcatccgcg
     7501 acatgacgcg gctgaccgag cgccacggcg cagacgagca gcgcatgctg gacgagggcg
     7561 agaagctcct gcgcggcctg atcgcgcagg acgactggct gccggaggat tacgcccgcg
     7621 cctcgcccgc gggctaccgg cagtacctcc tgcactgcga tccgatggag cgcttctcgg
     7681 tggtgagctt cgtctggatg ccgggccaga agacgccgat ccacgatcac acggtctggg
     7741 ggctggtggg ggtgatgcgc ggcgaggaga tttgcgagga gtacacccgc gaggtgaagc
     7801 ccgcgggccg ccacccggtg cgccgcgggc aggtggaccg cgtttcgccg cgcatcggcg
     7861 acatccacgt ggtcgccaac gagggcagcg aagtcgcggt gagcatccac gtgtacggcg
     7921 ccaacatcgg cgccgtgcgg cgccatacct acgatgccgc aaacggagcg cggcacgatt
     7981 tcgtctccgg ctacgccaac gccagcattc cgaacctgtg ggaccgctcc agggaagcgt
     8041 agcggcggcg ctgctggcgg cggcattcgt ggcgcaggcc tgcgaggtgc cgggtgacgg
     8101 cacctcgctg cggcgcgcgc tgctcaaggt caagtacctc cccgagaccg aagcctggga
     8161 gcgtgcggca cgcaagtccg cgacggtgca attcgtgctc tcgctcggcg ccccacacaa
     8221 ggtcgacggg cgctgctact ggccgatcga agcgcgcagc gacggcgcgc tctggaacac
     8281 gttctacgcc accccgagcg gcgagcacat cctggtcggc actcccggca gcggcctggt
     8341 gccgctcgac acctggcgca acgctgctcg atgacgcccg gcgagccgtt gcggcggccg
     8401 gtacgtttca gggcttgaac ctctcgtcga cgtccacgcg cagcccgttc gccagccggt
     8461 tggtctggtt ggcgagcccg accaccgcca tcagctccat cagcatcgcg tcgctcatgc
     8521 cccgcttgcg cgccgacgcg gtgtgggaat aggtgcaata ttcgcagccg ttggtggcgc
     8581 tcaccgcgac gtagatcatc tccttcacca gcggatcgag cgcgcccggc gccatcacct
     8641 gcttcacctg ctcccaggtg cggcgcagct gcgcggggtc atgggccagc gccttccaga
     8701 agttgttgat ccagtcgacg ccgcgcgttt tcatgatgtc gtcgtagacc gcgcgcactt
     8761 cggcgccggc agcatcgtat tcgatcaagg tgaccagtga cacgttgccc tccgtggtga
     8821 tcccggtata ttagccccat gctgctgaaa ggctcctgcc actgcggcgc ggtgcgcttc
     8881 tccctggagt cggagacgcc gtatccctac atgcactgct actgctcgat ctgccgcaag
     8941 accgcgggcg gcggcggcta cgccatcaac atcatgggcc aggccgccag cttcaagaag
     9001 cgcggccgca cgcgcatcta ccgtgcgacg cgcggcggcg gcgagcgcca tttctgcccg
     9061 cgatgcggca gcgcgctctg ggtctgggac aagcgctggc ccgaggggat ctacccgttc
     9121 gcctcggcga tcgatacgcg cctgcccgcg ccgaaggagc gccagcacat cctgctcggt
     9181 tcgaaggcga gctgggtccc ggcgcccagg ggtcggcacg acaagcactt ccgcgaatac
     9241 ccgcgcgagg cgatcatcga ctggcaccgc aagcgcggcc tgctgcgcta gcccatgccc
     9301 ggctggatct ccgcgctcag ccccgagacg ctcctcgtcg cgatcgccac gatgggcggc
     9361 atcgtcgcgc tgctgctcac cgtcgccgcg gtacgccggc tgcgcgcctg ggagatccgc
     9421 cgcggctcgg tgtatctgtt cctggccgcg gtggtggccg cgctcggcgt aggcggcgcg
     9481 atgctcgcgg ccagccttca cacctacgcc cggctcaccc acgagcagga ggcggcgcgc
     9541 gcggtgctgc gtgcgctcgg cccgcagcgc ttcgaactgc tgctggtgcg cgagggccag
     9601 ctgtcgcagc gtttcgagct gcgcggcgac gaatggcaga tcgacgcgcg cgtgctgaag
     9661 tggcgcggca tgggcgcgct cctcggcttc gacaccgtgt accgcttcga gcgtctctcg
     9721 ggccgctacg ccgacaacga cgccgagcgc aaggcgccac gcagcgtgca ttcgctgtcg
     9781 cccgacggca cggtggactt ctggcagcta ctgaagaagt accacggcta catgccgctc
     9841 gccgacgcgc tctacggcag cgcggcgtac gtgccgatgg ccgacggcgc gcagtacgtg
     9901 gtgagcgtgt cggcgaccgg gctgctgatc cggccgctga acgacacggc gaagcaggcg
     9961 gtcggcggct ggaaatgaag cgcttcgccg cgctcctggc gctcgccctg ctcgcggggt
    10021 gcgccggcag cggcgcgtac ttcagcgacg ccggcccgca gcccgcccgc gagccgcgcg
    10081 cgctcgccgc gtggccctgg caggaggtct ggaccggcgt cgtgttccag ggccgcaaag
    10141 tcggcttcgc gcgcctcgcg ctgcggcccg ccgccacgcc cgggcgctgg gagatcgaga
    10201 ccgagtccgc cctgcagctg cggtttctcg gcgtcgacaa gaaggtcggc ctgcgcgcgc
    10261 tcgacgaggt gcgcgccgac ctggcgctgg agcgcttccg ctacgagtac cacctcgacg
    10321 gcagccgcct cgccgtcgcc ggcgagcgcg acggcgacgc gctcgtggtg cgcaccgaca
    10381 gcagcggcgc gcgccgcgag cagcgcctga gtctcgacgc accgacggtg cccgcgagcg
    10441 cactcgcgct gctgccggca cagcgcggcc tgaacgtcgg cgagcgcgcg cgtgtcgccg
    10501 tcttcgtcgg cgagtcgcaa tccctcgagc aggccgagct gcgcgcgctg gcgcacgagc
    10561 gcagcccgct gttcgaaggc gttgcgctgc gcgtggccac cacgctgctc ggcctggaga
    10621 ccgaggcctg gtacgacgcc gaggcgcggc cgctgctcga gaccgcgctg cacggcgcca
    10681 tgctctcggc gctcgaagaa cccgcgcgcg cgcgggccta cctggtcgaa gcgagcctca
    10741 acaaggacga gtcgctgctc gaattcagcc tgctgcgctc gccgccggtg cgagaggcgc
    10801 ggcgcacggc cgcgttgacg ctttcgctcg agggcgtccc ggcggcgctc gacgtgccgg
    10861 gcacggccgg ccagcgctgc gtgcgcgatg gcgcgcgcct ggaatgccgc gtcgaccggc
    10921 gcacggcgcg gtccgcgggc gacgcgagcg aggcggcgtc ctacctgcgc ccgagcctcg
    10981 cggtgccaag caacgagcgc cgcttcttgg agctcgcgca gttgattgtc gccggcgcgc
    11041 tcgatgccga ggcgcgcatc gagcgcctgc ttgcgtggat ggacggaaac atcgccaagg
    11101 cggcgctcga tgcgttctcc gccgccgacg tgctgcgcga gcggcgcgcg gagtgccagg
    11161 gccatgccta cctgttcgcg gcgttcgcgc gcgcgctcgg cctgccgacc cgcgtggtga
    11221 atggcgtggt gtactccgcc gagcacggcg gctttctgta ccacacctgg aacgaggtct
    11281 ggctcgaggg cgccggctgg cgcgccgtcg accccacctt tggccagtcg cacgccgacg
    11341 cgacgcacgt cgcgctcgcc gtgggggaaa cgccggcggc gatcgcgccg ctcgcggcga
    11401 tggtggggcg cgcgcgcgtc gcggcgatcg gggaggtcgc gcactggtag aatgcgccgc
    11461 cgcgcatgaa gttcatcttc gacctgttcc cggtcatcct gttcgtcgcc acctacgtgt
    11521 ggacggacaa catctacatt gcgaccgccg tgatcattcc cgcgtcgatc gcccaggtgg
    11581 cgtacgtgtg gttccgttac cgccgcatcg agcgcatgct gctggtgtcg atggtgctgg
    11641 tgctgatcct gggcggcctc accctgttcc ttcaggacga gcgcttcatc aagtggaagc
    11701 cgacggtcct gtactggctg ttcgccgcgg tgctcgcgct ctcccccctc gtggcgaaga
    11761 agaacatcgt gcgcctgatg atggagaaag gcttcacggc gcctgagcgc atctggaact
    11821 ccctcaacgt cgcctgggtc ctgttcttcg tgttcatggg cgtgctcaac ctgtacatcg
    11881 cgcagaactt cagcctcggc ttctgggtgc agttcaagct ctggggtctc accgggctga
    11941 tgttcgcctt catcgccgcg cagatcgccg tcctgtacaa gtacgtggac gagcccgagg
    12001 ccaagaaatg agcacggcgg acgacatccg cgcgcgcctc gcggtactgc agccggagtc
    12061 gctcgagctc gaggacgaaa gcgagcgcca ccgcggccac gccggctacc ggccgggcgg
    12121 cggcacccac tggcgcctga gcatcgtcgc gcgcgcgttc gccggccagt ccaccgtcgc
    12181 gcgccaccgc ctcgtctacc aggcgctcgg cgggctgatg caggatccga tccacgcgct
    12241 cgccatccac gcgcgcgcgc cgggagaacc cggcgcgggc gcataatggc gggcaatcct
    12301 tctactcgtt actctctcga gaggcattcc atgaaattga gactccttgc tttcgcggcg
    12361 gccgccagcc tcgcgctccc cgcgctggcg cagcaggcca agcccgccgc caagccggcc
    12421 g
//
LOCUS       DBNHKE010000100        12701 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1338842, whole genome shotgun sequence.
ACCESSION   DBNHKE010000100 DBNHKE010000000
VERSION     DBNHKE010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1338842"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..892)
                     /locus_tag="AC16O9_06190"
     CDS             complement(89..892)
                     /locus_tag="AC16O9_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6516240.1"
                     /translation="MPHLEIAPGEALYYEYDAPGAAGATFVFVNALTGNTGTWQHADI
                     GPALRAAGYGTLAWNFRGQAQTRFGAQTALTPKLVVEDLQRVVAHTKPPKPIVVGLSI
                     GGLFAAQAVLAGMPASALVLINTLRKPGARLEWINQATVALARAGGTRLIMEANLPQL
                     VNPEQLAAMRAGVFGPEPYVPMDRADGLYRLLEGSLATDWNFPWERLKLPVLSMTGLH
                     DRVFYVAADVEALAAKIAGLRRVEFPDAGHLVPVERPAAFTRALLDFVK"
     gene            complement(895..2166)
                     /locus_tag="AC16O9_06195"
     CDS             complement(895..2166)
                     /locus_tag="AC16O9_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6516241.1"
                     /translation="MIRDRLFAVLNARAFYGWTILAVAGLGIFASGPGQSHTFSVFVE
                     PIGADLGLSKAAIAGAYGLATLGAAFLLPQMGKLVDRHGPRRMTLVVALALGLACMAF
                     GAAGGIVTLTLGFGALRFLGQGSLMMNCANLVAQWFSRKRGFAMGLMALGFAASMAVH
                     PPLGQFLINQVGWRWAWVILGLLTWALLLPPVLLLVHDKPEDKGLRPDGEAAPAAGAA
                     PPAIDGLTQQEALRTPTFYLLAAGWFGMAMLVTTLHFYQVSILTAQGVAAEIAARVFP
                     VSALVLAVTMPLVGRLFDHMRTRYVFAIGLVVMAAALFGVTLVESLASAIVYAALFGL
                     NNAFSLTMFGYLMPRYFGRKHVGSLQGTGQLVAVVGASLGPLPVGFAFDYLDSPTLVL
                     RLLALYPLACAVAAALYLRTPAGVTHPKHLE"
     gene            complement(2302..3555)
                     /locus_tag="AC16O9_06200"
     CDS             complement(2302..3555)
                     /locus_tag="AC16O9_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6516242.1"
                     /translation="MTSAIGFLVVGALMLAMGLLAPALQRLPLTTAIIYLAVGLLVGP
                     TVLGLFHFNPLKESAVLEVLTEAAVLISLFAAGVKLPAPLTWARWRSPVLLAFASMAL
                     TVVLIAAYGYYLLALPLGAAVLLGAILAPTDPVLAGDVQVRHPGDRDRLRFNLTCEAG
                     MNDGSAFPFVMLGLGLLGLHELGGFGLRWMLVDVLWATAGGIAIGVAGGVAVARLAAR
                     LRHAYPATERYDDLIALGMIGVVYGVAVIALTWGFLAVFFAAVALRQTELRIAGVADS
                     AGETEALPQVSVDALVFKEHLERVSEILLVLLIGGTLFLDSWSWRAVGLAAFVFLVAR
                     PVSVMGGLAGTRTPWPMRGMIGWFGVRGIGSLYYLMYAIEHGLPERLALEFIHLTLIV
                     VTLSILVHGVSVKPLMLRYWRGREG"
     gene            complement(3583..4785)
                     /locus_tag="AC16O9_06205"
     CDS             complement(3583..4785)
                     /locus_tag="AC16O9_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR6516243.1"
                     /translation="MSQPDTRPVILAVDDAEDLLALIGKALASEYRVKLASDGGDALT
                     LVAQAPQPDLVLLDVEMPGASGYEICQLLKEDPATAHIPVIFLTGRVEPSEQLEGLDV
                     GAVDYLTKPINAKLLMARVRIHLALSRRQAELERQVQDRTAQLEDARSQLIRRLARAM
                     EFHESAAAGNRVARVSQYAKLIAQAAGMKPEIAELLQKASPLYDIGKMGVPSEILRKP
                     GALSAPDIERMRRHPQLGAEIIGEHKDPVLSLAHKLALTHHERWDGTGYPKQLKGNDI
                     PWPGRVMALVDAFEAMTTTQFYRQKPYTIDEAAKLIRAEFGKQFDPSLAEAFGKALPA
                     MSKVREKFSDALGDLINLDFSPTRDAPAAAPPPKAPAPKAPPAKPDAAAKAAAAVAAA
                     KARLRDKR"
     gene            4970..5173
                     /locus_tag="AC16O9_06210"
     CDS             4970..5173
                     /locus_tag="AC16O9_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6516244.1"
                     /translation="MATGTVKWFNDAKGFGFITPDGGGKDLFAHFSAIQGSGFKSLKD
                     GQKVSFDVTTGPKGDQATNIKPM"
     gene            complement(5282..6265)
                     /locus_tag="AC16O9_06215"
     CDS             complement(5282..6265)
                     /locus_tag="AC16O9_06215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6516245.1"
                     /translation="MRICVFGAGAVGGHFAAQLAAAGHEVSVISRGDHLEAMRRNGLT
                     LLKGERRIVARVRAAERAADVGRVDCVLATLKATGLGALAAGIGPLLGPDTAVVFAQN
                     GIPWWYAQGLAGRPAPPAELERLDPGGALARAVAPERVLGGVIYSANEVLEPGVVRCN
                     APQRNVLVLGEPGGGTSARLAALSAALEAAEIRAPVEADIRRSVWAKLLINVGSSAIG
                     VVTGETVKAAMADPALAELRRRVHAEGRAVAAAHGVDPEGAPLPPPHQAGGPEHKTSM
                     LQDYERGRPMEIDAILMAPLWFARAAGVPAPALEALAALAAHKARAKGLYT"
     gene            6290..6676
                     /locus_tag="AC16O9_06220"
     CDS             6290..6676
                     /locus_tag="AC16O9_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6516246.1"
                     /translation="MRTFVKVVLFTDRDGRARFREEELLLAEGTPQALLSPVYPCAGY
                     QLRRSPVGFRSDWHCSPAPQWVFILQGEMEIGLRDGTSRVFRPGEHFFSSDTLPAGAA
                     AFDPEVHGHWSAQRGPAPLVTLFVKA"
     gene            6698..7471
                     /locus_tag="AC16O9_06225"
     CDS             6698..7471
                     /locus_tag="AC16O9_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6516247.1"
                     /translation="MAGRIYRMSTSGRRAWEKEDAAVPAVFRRILGMLDTETHGDVVR
                     GYLRRYPDQLIFEWLAELEELGLVESRPDKASHDLDFTGKFKLPRLIAEDRAQLARES
                     KAAGGVLERKGVYLATDRLQHRAPSEKAPAETVALIVEDDPDQLALAQLRVRMAGFAA
                     RTADSAAALQAALGRGDPPDVLLLDIGLPDGDGFHILAALRANPKHALLPVVMLTVKD
                     SPTDIERGLALGADGYIVKPYSKKLLEEVLSQVLRLDAG"
     gene            complement(7532..8155)
                     /locus_tag="AC16O9_06230"
     CDS             complement(7532..8155)
                     /locus_tag="AC16O9_06230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516248.1"
                     /translation="MTLDRHDLAEIAARTLADYGARAADFRRGTRDHDVSQNIGELLR
                     HLEGAPPFRILDLGCGPGRDLKAFRELGHVPVGLEGQPAFAAMARAHSGCEVLEQDFL
                     GLDLPPGSFDGVFANASLFHVPTQELPRVLGELKAALRPRGVLFSSNPHGRNEEGWSR
                     GRYGAFLDWETWKGLAEGAGFTALHHYYRPAGLPREQQPWLASSWRA"
     gene            8510..8674
                     /locus_tag="AC16O9_06235"
     CDS             8510..8674
                     /locus_tag="AC16O9_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516249.1"
                     /translation="MNMSAEEVKQFWRGYCERRKVAKDVVARGEAEIDKDPEFWADQT
                     MDQLLALVTR"
     gene            complement(8651..9505)
                     /locus_tag="AC16O9_06240"
     CDS             complement(8651..9505)
                     /locus_tag="AC16O9_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6516250.1"
                     /translation="MARVLVLVLCACLAPLAQAQRDAPRIAGAIELTDGDVLLEGRDG
                     KTRLPANGENVFEGDTVTTFPNGEIHLQMADGASLIGRENSKITIAEYVADGGDNDRS
                     LIDLAKGALRSITGWIGQYNRANYKIRTPLVTIGVRGTDHEPSHLLEGDPRGEPGSYD
                     KVNEGRTYMQGPQGTVDVPANRVVFRGHARGARARLLATTPGFFRPGRFERRFEGRAR
                     EVRRTIQERRQARREFLRPRGGPGTRPRPPERSLKDRPQRQRLEQRRDRGVEQRRERR
                     RSARDQRQ"
     gene            complement(9509..10291)
                     /locus_tag="AC16O9_06245"
     CDS             complement(9509..10291)
                     /locus_tag="AC16O9_06245"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6516251.1"
                     /translation="MSLVRPILAAALCLPLIAAAQPRAAGKIELAEGISRIQGKDQRE
                     RRPAVGEQVYEGDAIATAPRAELHLRMADGASIIVRERTKLTITAYVADGGAQDRSLL
                     DLAEGALRAISGWIGKYNRDRYAVRTPLVTIGVRGTDHEPTHLLQGDPRGKPGSYDKV
                     NEGLAVMQTPQGSLEIPANKAAFLGLEGVPPQLLDAVPDFFKPGKYEQEFTARARQVE
                     RALDELRKGRVEELLKLKDGKLNLPAAPTRDSLRRGLERFLK"
     gene            10329..11867
                     /locus_tag="AC16O9_06250"
     CDS             10329..11867
                     /locus_tag="AC16O9_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HIR6516252.1"
                     /translation="MAAVLTPERPVDLSAASFRSSFVDEMPADPLASNTRRQVPNASY
                     TRVTPTPVAAPNLLAWSQALAGELGLARPSAQALEALAGNRVFAGMAPYASRYGGHQF
                     GVWARQLGDGRAITLGELVARDGSRQELQLKGAGLTPYSRTADGRAVLRSSLREYLCS
                     EAMHFLGVPTTRALSLVGTGESVIRDMFYDGNPEPEPGAVVCRVAPSFLRFGNFEIHA
                     AHGEQELLKRLLDYVIRHFYPGLSYRDWYHEVCRRTARLMADWMRLGFVHGVMNTDNM
                     SVLGLTIDYGPYGWLEGYDPAWTPNTTDNETRRYCYGNQPQIGLWNLARLAEALLPLI
                     AKEDLEAGLEVYGNVYQESFSRALAQKLGLAAFDGDDDEALLAELFQVLNAVETDFTL
                     FFRGLSAVREDALVAPLARAFYAAPPATHVARLEAWLRRWLARGPERARMNAVNPKYV
                     PRNYLAQQAIDAMAAGDASVLERLMRVLERPYDEQPGHDDLAERRPEWARHKPGCSAL
                     SCSS"
     gene            11819..12622
                     /locus_tag="AC16O9_06255"
     CDS             11819..12622
                     /locus_tag="AC16O9_06255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516253.1"
                     /translation="MGAAQAGLLGAVVQLLRLALALLLAAGASIGAAQQSAPYEPTFG
                     QQGKDVVWVPTVPALVEKMLDIAQVGPQDVVFDLGSGDGRTVIAAARRGARAVGIEYD
                     GNMVALSRRNAAQEGVAGRATFAQADLFKTDLSPATVITMFLLPTINMKLRPTLLGLK
                     PGTRIVSNTFTMDKWQPDASAVLDQDTGCANWCTALLWIVPAQVAGRHVLPQGELELK
                     QEFQVLAGTLRQNGVTYELEGQVRGEALAFRAGGREYRGRVNGKTIELR"
BASE COUNT         1921 a         4453 c         4495 g         1832 t
ORIGIN      
        1 cccggccaga aaaagccgag atgtacgaaa ggcagaaccc gcgcggacgg tggtgaggcc
       61 gccagctcaa ataaaatgag gtctgtccct atttaacgaa gtccagcagc gcccgggtga
      121 atgccgccgg ccgctccacc ggcacgagat gcccggcgtc gggaaactcg acacgcctca
      181 gcccggcgat cttcgccgcg agcgcctcca cgtccgccgc aacgtagaac acccggtcgt
      241 gcagtccggt catcgaaagg accggcagct tcagcctttc ccacggaaag ttccagtcgg
      301 tggcgagcga gccctcgagc aggcgataga gtccgtcggc gcggtccatc ggcacgtacg
      361 gctcggggcc gaacaccccg gcgcgcatcg ccgcgagctg ctcgggattg acgagctgcg
      421 gcaggttggc ttccatgatc aggcgcgtgc cgccggcgcg cgccagcgcg acggtcgcct
      481 ggttgatcca ctcgagccgc gcgccgggtt tgcgcaaggt gttgatgagc acgagtgccg
      541 acgccggcat gccggcgagc accgcctgcg ccgcaaagag gccgccgatg gacaggccta
      601 cgacgatcgg cttgggcggc ttcgtatgcg cgacgacgcg ctgcaagtcc tccaccacca
      661 gctttggcgt gagcgcggtc tgcgcgccga agcgcgtctg cgcctggccg cggaaattcc
      721 acgcgagcgt gccgtagcct gcggcgcgca gggccgggcc gatatcggcg tgctgccagg
      781 tcccggtatt gccggtgagc gcgttgacga agacgaacgt ggcaccggcg gcgccaggcg
      841 catcgtattc gtagtagagg gcttcgccgg gggcgatttc gaggtggggc atggctactc
      901 caggtgcttg gggtgggtca cgcccgcggg cgtgcgcaga tagagcgccg cggcgacggc
      961 gcaggcaagc ggatacagcg caagcagccg cagcacgagc gtcgggctgt cgaggtaatc
     1021 gaaggcgaag cccaccggca gcggcccgag cgaggcgccc accaccgcca ccagctggcc
     1081 ggtgccctgc aacgacccga catgcttgcg cccgaagtag cgcggcatca ggtagccgaa
     1141 catggtgagg ctgaacgcgt tgttcaggcc gaagagcgcc gcgtagacga tcgcgctcgc
     1201 caggctctcg accagcgtca cgccgaagag cgccgccgcc atcaccacca gcccgatcgc
     1261 gaacacatag cgcgtgcgca tgtgatcgaa cagccggccg acgagcggca tggtcaccgc
     1321 cagcaccagc gccgacaccg ggaacacgcg cgcggcgatc tcggcggcca caccctgtgc
     1381 ggtgagaatg gacacctgat agaagtgcag cgtcgtgacg agcatcgcca tgccgaacca
     1441 gccggcggcg agcaggtaga acgtcggcgt gcgcagcgcc tcctgctgcg tcaggccgtc
     1501 gatcgcgggc ggcgccgcgc ccgcggccgg cgcggcctcg ccgtcggggc gcagtccctt
     1561 gtcctcgggc ttgtcgtgca ccagcagcag caccggcggc aggagcagcg cccaggtcaa
     1621 gagacccagg atcacccagg cccagcgcca gccgacctgg ttgatgagga actggccgag
     1681 cggcgggtgc accgccatcg aggcggcgaa gccgagcgcc atcaggccca tggcgaagcc
     1741 gcgcttgcgg ctgaaccact gcgcgacgag gttcgcgcag ttcatcatca gcgagccctg
     1801 gccgagaaag cgcagcgcgc cgaagccgag cgtgagcgtg acaatgccgc cggcggcgcc
     1861 gaatgccatg caggcaagcc cgagcgcgag cgcgacgacg agcgtcatgc gccgcgggcc
     1921 gtggcggtcg accagcttgc ccatctgcgg cagcaggaac gccgcgccca gggttgcgag
     1981 gccgtacgcg ccggcgatgg cggccttcga caggccgagg tcggcgccga tcggctcgac
     2041 gaacacgctg aaggtgtgcg actgcccggg gccgctggcg aagatgccca gccccgccac
     2101 cgcgaggatg gtccagccgt agaaggcgcg cgcgttgagc acggcgaaca ggcggtcgcg
     2161 gatcacgtcc gggattctaa cggggccggc gacgcttacg cgtcggagcc tccgttgggt
     2221 tttcttttgt ctcggctttt ctggccggat gaatctcccg gccagaaaag ccgagatcga
     2281 tttgaaaggc aaacgcaccg ttcacccctc gcgcccccgc cagtaccgca gcatcagcgg
     2341 cttcacgctc acgccgtgca cgaggatcga cagcgtcacc acgatcagcg tgaggtggat
     2401 gaactccagc gccaggcgct cgggcagccc gtgctcgatg gcatacatca ggtagtacag
     2461 ggagccgatg ccgcgcacgc cgaaccagcc gatcatgccg cgcatcggcc agggcgtgcg
     2521 cgtgccggcg agcccgccca tcacgctcac cggccgtgcg acgaggaaca cgaacgcggc
     2581 gagccccacc gcgcgccagc tccacgagtc gaggaacagc gtgccgccga tgagcagcac
     2641 cagcaggatc tcggagacgc gctcgaggtg ctccttgaac accagcgcgt cgacgctgac
     2701 ctgcggcagc gcctcggtct cgccggcgct gtcggccacc ccggcgatgc gcagctcggt
     2761 ctggcgcagc gccaccgcgg cgaagaacac ggcgaggaag ccccaggtca gcgcgatcac
     2821 cgccacgccg tacaccacgc cgatcatgcc gagggcgatc aggtcatcgt agcgttccgt
     2881 ggccgggtag gcgtggcgca gccgggccgc gaggcgcgcg accgctacgc cgcctgcgac
     2941 gccgatggcg atgccgcccg cggtggccca gagcacgtcg accagcatcc agcgcagccc
     3001 gaagccgccg agctcatgca ggccgagcag gccgagcccg agcatcacga acgggaacgc
     3061 gctgccgtcg ttcatgccgg cttcgcaagt gaggttgaag cgcagccggt cgcggtcgcc
     3121 cgggtggcgc acctgcacgt cgccggcgag caccgggtcg gtcggcgcga ggatcgcccc
     3181 gagcagcacc gcggcgccga gcggcagcgc gagcaggtag tagccgtagg ccgcgatcag
     3241 gaccacggtc agcgccatcg aggcgaacgc gagcagcacc ggcgagcgcc agcgcgccca
     3301 ggtgaggggc gcgggcagct tcacgcccgc ggcgaagagc gaaatcagca ccgccgcctc
     3361 ggtcagcacc tcgagcaccg ccgattcctt gagcgggttg aagtggaaca ggccgagcac
     3421 cgtcggtccg acgagcaggc cgacggcgag gtagatgatg gcggtggtga gcggcaggcg
     3481 ctgcagcgcc ggggcgagca gccccatggc gagcatcagc gcgccgacga cgagaaaacc
     3541 gatggcgctg gtcatgcgcg cggacggcgg ccctttgcag ggctagcgct tgtcgcgcag
     3601 ccgggccttg gcggcagcca ccgccgccgc ggccttggcg gccgcgtcgg gcttggccgg
     3661 cggcgccttg ggtgccgggg ccttgggcgg cggcgcggct gccggcgcgt ctcgcgtcgg
     3721 gctgaagtcg aggttgatca ggtcgccgag cgcgtcggag aatttttcgc gcaccttgct
     3781 catcgcgggc agcgccttcc cgaacgcctc ggcaagcgaa gggtcgaact gcttgccgaa
     3841 ttcggcccgg atcagcttcg ccgcctcgtc gatcgtgtag ggcttctggc ggtagaactg
     3901 ggtcgtggtc atcgcctcga atgcgtcgac cagcgccatg acgcgccccg gccaggggat
     3961 gtcgttgccc ttcaactgct tcgggtagcc ggtgccgtcc cagcgctcgt ggtgcgtgag
     4021 cgcgagcttg tgcgcgagcg agagcaccgg gtccttgtgc tcgccgatga tctcggcgcc
     4081 gagctgcggg tggcgccgca tgcgctcgat gtcgggcgcc gagagcgcgc cgggcttgcg
     4141 caggatctcg gacggcaccc ccatcttgcc gatgtcgtac agcggcgagg ccttctgcag
     4201 caactcggca atctccggct tcatgccggc cgcctgggca atgagcttgg cgtactggct
     4261 gacccgcgcg acgcggttgc cggcggcggc gctttcgtgg aactccatgg cgcgggcgag
     4321 ccgccggatg agctgcgagc gcgcgtcctc gagctgcgcg gtgcgatcct gcacctggcg
     4381 ctcgagttcg gcctgccgcc gggagagcgc aaggtggatg cgcacgcgcg ccatgaggag
     4441 cttggcattg atcggcttgg tgaggtagtc gaccgcgccg acgtcgagcc cctcgagctg
     4501 ctcggagggc tccaccctgc cggtgaggaa gatcaccgga atgtgcgccg tggcggggtc
     4561 ttccttcagc agctggcaga tctcgtagcc gctcgccccc ggcatctcca cgtcgagcag
     4621 gaccagatca ggctgtggcg cctgcgcaac gagcgtcagg gcgtcgccgc cgtcgctggc
     4681 gagcttcacc cggtattccg aagcgagcgc cttgccgatc agggcgagca ggtcttcggc
     4741 gtcgtccacc gccaggatga caggccgggt gtcgggctgg ctcacgccgg caagtctacc
     4801 tcctggatca cggcagtgct tgacgcaaaa ggcgttcatg tataatgcgc gccgctggtt
     4861 tcctcaagca gttccgcagg ctcttgcatc tcccgcgtct tctacttggt gttccggcag
     4921 tgggcgaaag cccgtgaccc gttaactttt tttacaaagg caattcgaca tggcaactgg
     4981 aacagtcaag tggttcaacg acgcaaaggg cttcggcttc atcaccccgg acggcggcgg
     5041 caaggacctg ttcgcgcatt tctcggcgat ccagggctcg ggcttcaaga gcctgaagga
     5101 cggccagaag gtctccttcg acgtcaccac gggaccgaaa ggcgaccagg cgacgaacat
     5161 caagccgatg taactgtcgg cttcaggcag caaacaaaaa ggcccgcgca agcgggcctt
     5221 tttaattggg gacagacctc atttacgcat ctgctttgcg taaatgaggt ctgtcccctt
     5281 tttacgtgta caggcccttg gcgcgcgctt tgtgcgccgc gagcgcggcc agggcctcga
     5341 gggccggcgc cggcacgcca gccgcgcgcg cgaaccacag cggcgccatc aggatcgcgt
     5401 cgatctccat cggccggccg cgctcgtagt cctgcagcat cgaggtcttg tgctccgggc
     5461 cgccggcctg gtgcggcggc ggcagcgggg cgccttcggg gtcgacgccg tgcgccgcgg
     5521 ccacggccct gccctcggcg tgcacgcggc ggcgcagctc ggcgagcgcc gggtcggcca
     5581 tcgccgcttt caccgtctcg ccggtcacca ccccaatggc cgagctgccg acgttaatga
     5641 gcagcttggc ccataccgag cggcggatgt cggcttcgac gggggcccgg atctcggcgg
     5701 cttcgagcgc cgcgctcagc gcggcaagcc gcgccgatgt gccgccgccg ggctcgccga
     5761 ggacgaggac gtttctctgc ggcgcgttgc agcgcaccac gcccggctcg agcacctcgt
     5821 tggccgaata gatcacgccc ccgagcacgc gctcgggcgc cacggcgcgc gcgagtgccc
     5881 cgccgggatc gagccgctcg agctcggccg gcggcgccgg cctgccggcc agcccctgcg
     5941 cgtaccacca ggggatcccg ttttgcgcga agaccacggc cgtgtccggc ccgaggagcg
     6001 gcccgatgcc cgcagcgagc gcgccgaggc cggtggcctt cagcgtcgcc agcacgcagt
     6061 ccacgcggcc gacgtcggcc gcgcgctccg ccgcgcgcac ccgggcgacg atgcgccgct
     6121 cgcctttcag cagggtgagc ccgttcctgc gcatcgcctc gaggtgatct ccccgggaaa
     6181 tcaccgacac ttcgtgcccc gccgcggcga gctgtgcggc aaaatggccg ccgaccgccc
     6241 cggcgccgaa aacgcatatc ctcacgccgc ccacccgctg gagaccgcca tgcgcacctt
     6301 cgtcaaagta gtgctgttca ccgaccgcga cggccgcgcg cgctttcgcg aggaggagct
     6361 gctgctcgcc gaaggcacgc cgcaggcgct gctctcgcca gtgtacccgt gcgccggcta
     6421 ccagctgcgg cgcagcccgg tgggattccg cagcgactgg cattgctcgc cggcgccgca
     6481 atgggtgttc atcctgcagg gggagatgga gatcgggctg cgtgacggca cctcgcgcgt
     6541 gttccggccc ggggagcatt tcttctcgtc cgacacgctg cctgcgggcg ccgcggcgtt
     6601 cgatccggag gttcatggcc actggagcgc gcagcgcggg ccggcgccgc tggtcacgct
     6661 ctttgtaaaa gcctgaggcc cgcctatagt tgcgcgcatg gccgggcgca tctatcgcat
     6721 gtccacgtcc gggcggcgcg cgtgggagaa ggaggacgct gcggtgcccg ccgtgttccg
     6781 tcgcatcctc ggcatgctcg acaccgagac gcacggcgac gtggtgcggg gctacctgcg
     6841 ccgctatccc gaccaactga tcttcgagtg gctggcggag ctcgaggagc tcggcctggt
     6901 cgagtcgcgc cccgacaagg cgtcgcacga cctcgacttc accggcaaat tcaagctgcc
     6961 ccggctgatc gcggaggacc gcgcgcagct cgcgcgcgaa tcgaaggctg ccggcggcgt
     7021 gctcgagcgt aagggcgtat accttgccac cgatcggctg cagcaccggg ccccgtccga
     7081 gaaggcgccc gccgagaccg tggcgctgat tgtcgaggac gaccccgacc agctcgcgct
     7141 ggcgcagctg cgcgtgcgca tggcgggctt tgctgcgcgc accgcggatt cggccgccgc
     7201 gctgcaggcg gcgctcggcc gcggcgatcc gcccgatgtg ctgctgctcg acatcgggct
     7261 tcccgacggc gacggcttcc acatcctggc ggcgctgcgc gcgaacccga agcacgccct
     7321 gctgccggtg gtgatgctga cggtcaagga cagccccacg gacatcgagc gcgggctggc
     7381 gctgggcgcc gacggctaca tcgtcaagcc ctacagcaag aagctgctcg aagaggtgct
     7441 gtcgcaggtg ttgcgcctgg acgcgggctg aggggcgccg gtcagcccct gagccgcacg
     7501 gcgagcggca tgccgccccg gggaacgagc gtcacgcgcg ccagctgctc gcgagccacg
     7561 gctgctgctc gcgcggcagc cccgcggggc ggtagtagtg gtgcagcgcg gtgaagccgg
     7621 cgccctcggc caagcccttc caggtttccc aatcgaggaa cgcaccgtag cgcccgcggc
     7681 tccagccctc ttcgttgcgg ccgtgcggat tcgacgagaa cagcacgccg cgcggcctga
     7741 gcgcggcttt cagttcgccg agcacgcgcg gcagctcctg ggtgggcacg tgaaacagcg
     7801 aggcgttcgc gaaaacgccg tcgaacgagc ccggcggcag atcgaggccg agaaaatcct
     7861 gctcgagcac ctcgcaaccg ctgtgtgcgc gcgccatggc cgcgaacgcc ggctgcccct
     7921 cgagtcccac cggcacatgg ccgagctcac ggaacgcctt caggtcccgg cccggcccgc
     7981 agcccagatc gagtatgcga aacggcggcg ccccttccag gtgtcgcagc agctcgccga
     8041 tgttctggct gacgtcgtgg tcccgggtgc cgcggcgaaa gtcggccgca cgcgcgccgt
     8101 agtccgccag cgttcgagca gcgatctccg ccaggtcgtg ccgatcaagc gtcatgcggc
     8161 gtccatttgt ccgataatgc gtcaatcatc aggagctctc gaatgcccaa gggaacgcag
     8221 cgcagcaacc gcgagaagaa gaagcccaaa cagcccccga agcccctcgc ccccggcaag
     8281 cccgtgcgcc agccctggaa ataggcgtcg caaatgaaac gccccggcca gtcgcccggc
     8341 cggggcgttt gctaaggcat ggccggctgc tctagctggc gatgctcctt tgaccactga
     8401 atgatccggt tcgggagccc agcctgccgg tgctgccttg gtcgtctctc actggatcac
     8461 ctcctttcgt ggttgccagc ccgcagtgta gcaggacaga attccgccca tgaacatgtc
     8521 tgccgaggaa gtgaagcagt tctggcgtgg ctattgcgag cgccggaagg tagcgaagga
     8581 cgtcgtcgcc aggggcgagg cggagatcga caaggatccg gagttctggg ccgaccagac
     8641 gatggaccag ctactggcgc tggtcacgcg ctgaacggcg ccgctcgcgg cgctgctcca
     8701 ccccgcggtc gcggcgctgc tccagccgct ggcgctgcgg gcggtccttc agggagcgct
     8761 cgggcggccg cgggcgcgta ccgggcccgc cccgcgggcg caggaactcg cgccgcgcct
     8821 ggcgccgctc ctggatcgtc cggcgcacct cgcgcgcgcg gccctcgaag cggcgctcga
     8881 accgtcccgg cctgaagaac cccggcgtcg tggcgaggag gcgcgcgcgc gcgccgcgcg
     8941 cgtgcccgcg gaacacgacg cggttcgccg gcacgtccac cgtgccctgc gggccctgca
     9001 tgtaggtgcg gccttcgttc accttgtcgt agctgccggg ctcgccgcgc gggtcgccct
     9061 cgagcaggtg cgagggctcg tggtcggtgc cgcgcacgcc gatggtgacg agcggcgtgc
     9121 ggatcttgta gttcgcgcgg ttgtactggc cgatccagcc ggtgatcgag cgcagcgccc
     9181 ccttggcgag atcgatcagg ctgcggtcgt tgtcgccgcc gtcggcgacg tactcggcga
     9241 tggtgatctt ggagttctcg cgcccgatga gcgaagcccc atcggccatc tgcaggtgga
     9301 tctcgccgtt cgggaacgtg gtcaccgtgt cgccctcgaa cacgttctcg ccgttcgcgg
     9361 gcaggcgggt cttgccgtcc cggccctcga gcagcacgtc gccgtcggtg agctcgatcg
     9421 cgccggcgat gcgcggcgcg tcgcgctgcg cctgcgcgag cggcgcgagg cacgcgcaca
     9481 agacgagaac gaggacgcgc gccacggact atttcaggaa ccgctccagg ccgcggcgca
     9541 ggctgtcgcg cgtcggcgcc gcgggcaggt tgagcttgcc gtccttcagc ttcagcagct
     9601 cctcgacgcg gcccttgcgc agctcgtcca gcgcgcgctc cacctgccgc gcccgggcgg
     9661 tgaactcctg ctcgtacttc cccggcttga agaaatcggg aaccgcgtcg aggagctgcg
     9721 gcggcacgcc ctcgagcccg agaaacgcgg ccttgttggc ggggatctcg aggctgccct
     9781 gcggggtctg catcaccgcc agcccttcgt tcaccttgtc gtagctcccc ggcttgccgc
     9841 gcgggtcgcc ctggaggagg tgcgtgggct cgtggtcggt gccgcgcacg ccgatggtga
     9901 cgagcggcgt gcgcacggca tagcggtcgc ggttgtactt gccgatccag ccggagatcg
     9961 cgcgcagcgc cccctcggcg aggtcgagca ggctgcggtc ctgcgcgccg ccgtcggcga
    10021 cataggcggt gatcgtcaac ttcgtgcgct cgcgcacgat gatcgaggcg ccgtcggcca
    10081 tcctcaggtg cagctccgcc ctgggcgcgg tggcgatggc gtcgccctcg tagacctgct
    10141 cgccgacggc gggacggcgc tcgcgctggt cctttccctg gatgcgcgag atgccttcgg
    10201 cgagctcgat cttgccggcg gcgcgcggct gcgctgcggc gatgagcggg aggcagagcg
    10261 cggcggcgag gatcggcctg acgagggaca tcgcgaccgg atcatacctg ctgcgttacc
    10321 atcgtcccat ggccgccgtc cttacgcctg aacgacccgt cgacctgtcc gcggcctcgt
    10381 tccgcagctc cttcgtcgac gagatgcccg ccgacccgct cgcgtcgaac acccgccgcc
    10441 aggtcccgaa cgcgagctac acgcgcgtca cgcccacccc ggtggcggcg ccgaatctgc
    10501 ttgcgtggtc gcaggcgctg gcgggcgagc tgggactcgc gcggccctcg gcccaagccc
    10561 tcgaggcgct cgccggcaac cgcgtgtttg cgggcatggc gccctacgcc tcgcgctacg
    10621 gcggacacca gttcggcgtc tgggcgcgcc agctcggcga cggccgcgcc atcacgctcg
    10681 gggagctcgt cgcgcgcgac ggctcgcgcc aggagctgca gctgaagggc gcggggttga
    10741 cgccctactc gcgcaccgcg gacgggcgcg cggtgctgcg ctccagcctg cgcgagtacc
    10801 tgtgcagcga ggcgatgcac ttcctgggcg tgccgaccac gcgcgcgctg tcgctcgtcg
    10861 gcacgggcga atcggtgatc cgcgacatgt tctacgacgg caaccccgag cccgagccgg
    10921 gcgcggtggt gtgccgcgtc gcgccgtcct tcctgcgctt cggcaacttc gagatccacg
    10981 ccgcgcacgg cgagcaggag ctgctcaagc gcctgctcga ctacgtcatc cggcatttct
    11041 atccgggcct ttcgtaccgg gactggtacc acgaggtgtg ccgccgcacc gcgcggctga
    11101 tggccgactg gatgcgcctg gggttcgtgc acggcgtgat gaacaccgac aacatgtcgg
    11161 tgctcgggct caccatcgac tacgggccgt acggctggct cgagggctac gacccggcct
    11221 ggacgccgaa caccacggac aacgagacgc ggcgctactg ctacggcaac cagccgcaga
    11281 tcgggctgtg gaacctggcg cggctggccg aggcgctgct gccgctgatc gcgaaggagg
    11341 acctggaggc cgggctcgag gtctacggca acgtctacca ggaaagcttt tcccgcgcgc
    11401 tggcgcaaaa gctcgggctc gccgcgttcg acggcgacga cgacgaggcg ctgctcgccg
    11461 agctgttcca ggtgctgaac gcggtggaga ccgatttcac gctgttcttc cgcgggcttt
    11521 ccgcggtgcg cgaggacgcc ctcgttgcgc cgctcgcgcg cgcgttctac gccgcgccgc
    11581 cggcgacgca tgtcgcgcgg ctcgaggcct ggctgcggcg ctggctcgcc cgcgggcccg
    11641 agcgcgcccg catgaacgcc gtcaacccga agtacgtgcc gcgcaactac ctcgcgcagc
    11701 aggccattga cgccatggcc gcaggagatg cctcggtgct ggagcggctg atgcgggtgc
    11761 tcgagcggcc ctacgacgag cagcccgggc acgacgacct cgcggaaaga aggcccgagt
    11821 gggcgcggca caagccgggc tgctcggcgc tgtcgtgcag ctcctgaggc tcgcccttgc
    11881 tttgctgctg gccgccggcg cctccatcgg cgccgcgcag cagagcgccc cttacgagcc
    11941 cacgttcggg caacagggca aggacgtcgt ctgggtgccc acggtgccgg cactggtcga
    12001 gaagatgctc gacatcgcgc aggtcggacc gcaggacgtg gtcttcgacc tcggctcggg
    12061 cgacggccgc accgtgatcg ccgccgccag gcgcggcgcg cgcgccgtgg gcatcgagta
    12121 cgacgggaac atggtcgcgc tttcgcggcg caacgcggcg caggaaggcg tcgccgggcg
    12181 cgccaccttc gcgcaggcgg acctgttcaa gaccgacctc tcgccggcga cggtgatcac
    12241 catgttcctg ctgccgacga tcaacatgaa gctgcgcccc acgctgctcg gcctgaagcc
    12301 cggcacgcgc atcgtgtcca acaccttcac catggacaag tggcagccgg atgcctccgc
    12361 ggtgctcgac caggacaccg gctgcgccaa ctggtgcacg gcgctgctgt ggatcgtgcc
    12421 ggcgcaggtc gcggggcgcc acgtcctgcc gcagggcgag ctggagctga agcaggagtt
    12481 ccaggtgctc gcgggcacgc tgcgccagaa cggcgtgacc tacgagctcg agggccaggt
    12541 gcgcggcgag gcgctcgcgt ttcgcgcggg cggccgcgag taccgcggcc gcgtgaacgg
    12601 caagacgatc gagctgcgct agcgccgggc cggctcgagc gcctgcgccc gggcgccgaa
    12661 gccctgggca tcgcgctcaa ggcgcaggcc gaacgtgcgc t
//
LOCUS       DBNHKE010000101         9412 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1392057, whole genome shotgun sequence.
ACCESSION   DBNHKE010000101 DBNHKE010000000
VERSION     DBNHKE010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1392057"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /gene="kdsB"
                     /locus_tag="AC16O9_06260"
     CDS             <1..551
                     /gene="kdsB"
                     /locus_tag="AC16O9_06260"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR6516254.1"
                     /translation="HGFEARMTRADHASGTDRIAEAAAQLGLADDAIVVNVQGDEPLI
                     APQLIAGVAAALAARPEASVATACHPIHDPAEFANPNVVKVVRAASGDALYFSRAGIP
                     FPRDGGTPAAERHIGIYAYRVAFLRRYAGLAPAPLESTEQLEQLRVLWHGHRITVVQA
                     EGAMAPGVDTPADLEAVLKLLR"
     gene            548..1201
                     /gene="adk"
                     /locus_tag="AC16O9_06265"
     CDS             548..1201
                     /gene="adk"
                     /locus_tag="AC16O9_06265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - AMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR6516255.1"
                     /translation="MRLILLGPPGAGKGTQAAFITEKYGIPQISTGDMLRAAAEAGTP
                     LGLAAKKLMDGGQLVPDEVVVALVEERLRAPDCARGYLLDGFPRTIPQAEAMRKAALP
                     LERVLELDVPDEEIVARMSGRRVHPASGRSYHQQFNPPQTPDRDDVTGEPLIQREDDR
                     EATVRKRLEVYRRQTRPLVEYYTRWAASGDARAPRVHCISGSGGIEEIRDRAFAALG"
     gene            1266..3335
                     /locus_tag="AC16O9_06270"
     CDS             1266..3335
                     /locus_tag="AC16O9_06270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR6516256.1"
                     /translation="MAGGLGFALICALLAIVYGVWQSAQILRLPDGNEKMRQIAAAIR
                     EGAVAYLWRQYKTIAMVGAVLFLAIGVTPQLGWTTAWGFLFGAVLSGACGIIGMNISV
                     RANVRTAEAARVGLNEALQVAFKGGAITGLLVVGLGLAGVAGLFALLYANALKKDDLV
                     HIIHPLIGFGFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETYAVTIVATMLLGALMLKTEAAVIYPLAIGGFAIIASIIG
                     TWFVKAKPGEKNVMPALYKGLAVAGGVALIAFWPITQVVMGDLLKTTQFDIAGAKTTI
                     GVWHLYGTAVVGMALTGFLVWITEYYTGTQYKPVQHVAQASTTGHGTNIIAGLGVSMK
                     STSWPVLAVCVAIIASYALAGLYGIAIAATSMLSMAGIIVALDAYGPITDNAGGIAEM
                     ADLPESVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALEAAGRSVSFD
                     LSNHMVIVGLFIGGLVPYLFGAMAMEAVGRAAGSVVVEVRRQFKEIPGIMEGKAKPEY
                     GVAVDMLTRAAIKEMMIPSLLPVLVPIVVGVVLGPQALGGVLMGTIVTGLFVAISMCT
                     GGGAWDNAKKYIEDGNHGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVAL
                     LVVPLMV"
     gene            3332..4999
                     /gene="ggt"
                     /locus_tag="AC16O9_06275"
     CDS             3332..4999
                     /gene="ggt"
                     /locus_tag="AC16O9_06275"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6516257.1"
                     /translation="MSVRESHLRRAACAALFFFCAGVGAQLAAPEVASERVEREAAYA
                     KRFMVAAADPRAVDAGLEMLRAGGSAADAAIAAQLVLGLVEPQSSGLGGGAFVLTWSE
                     KDKRVRSFDAREIAPATARPDRFLDRHGNPEQFMHAVSSGRSVGVPGVPRLLEVLHRE
                     HGRLAWPRLFAPAIRLAERGFEKTSRLARQLEREALLGADPAARALFYAGERIANPDY
                     AATLRALAGQGADAFYRGAIAADIVRAVNADLALADLAGYRVIERDPVCGRYRGYRIC
                     GMGPPSSGATGVLQILGILERTAFERAAPGSAEAVHLFSEAARLAYADRALYVGDPDF
                     APVPVSRLLEPRYLAARARLIGERSMGFAFPGTLDAETPGTSHLSVVDGEGNAVAMTT
                     SIEMGFGSRILVRGFLLNNQLTDFSFRPEALGRPAVNRVEGGKRPRSSMAPTLVFERD
                     GRLKMALGSPGGLAIINFVAKTLVAALDWGMDMQAAISSPNFGSTNGPTFVERGTAYE
                     ALGDALAERGHVLNFARLTSGLHAIERVPGGWRGGADPRRSGKAAGD"
     gene            5298..5495
                     /locus_tag="AC16O9_06280"
     CDS             5298..5495
                     /locus_tag="AC16O9_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6516258.1"
                     /translation="MLTPREREVLRHIAQGLSYAEVGTELGVSPNTVTSHVKNAYRKL
                     DVHTAAAAVMRAVELRLIGQQ"
     gene            5576..8650
                     /locus_tag="AC16O9_06285"
     CDS             5576..8650
                     /locus_tag="AC16O9_06285"
                     /inference="COORDINATES: protein motif:HMM:NF052741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR6516259.1"
                     /translation="MNAPASRRRSPLGKTTRPLLAQILPRERVFALLDEASDMPLTWV
                     SGPPGSGKTTAVASWLDHAPQRTLWYQVDEGDADPATFFYYLGLAVADLEGAEHPALP
                     LLTPEYHAGLVAFTRRYAQELYARLKPPFVIVFDGYHEVPASSPLHEAMREALRELPP
                     GGRAIVVSRADPPAALARLRANQQLAHVGWEELRLTREETVSVAAQRRPELAADALAG
                     LYDKTQGWAAGLVLLLQHARMGAPIVDAPDFATRQLVFDYLAGEILQKSDARTQEFLL
                     RTAHLPQMTAALAQQLSGEAAAGEMLARLHRENYFVALREAHAEPVYQYHPMFREFLL
                     ARAQEAYPKDRRRQLQKDAAALLEGAGQIEEAVALARDAHDWDAMARLIERHAGAMFA
                     QGRAETLVHWVEELPPEVQQAHPWTVYWAAASQAQRAPREGRLGFERAYELFRTAAAP
                     DSAGRVLACSGAMDAILYELDDFSLLDRWIAVLDAMALEGMRLPTPEAEARVACSMFI
                     ALTLRQPQRRDIEQWIQRAIAASQQVPDTNLRMFVGLLAALTLMWTGVLVRADGLIEA
                     MRKLAATPGVSPFSLLTLKNVEAMYHMLTAAPEPCLAAMREGQEIARATGIHTWSLQL
                     LVNGYGGALGGGDLAAAAPLARQLESQASGAGRFNLCLYHHFQAWEAALRKDLMRAHE
                     HERRALRMAIEVGCPFLEVLTRLALAQVLAECGDERKCISHLTQMRAIARTIDSHHLE
                     YTCLLGFAGIALEHGRSRPGLNALRRGFALGREHNYRHFLWWRPTAIARLCEHALAAG
                     IETEYAKSLVKRRALAPERPPLDVPEWPWHFRVHTLGGFRLLRHGEPLAAATGKAQRR
                     PLELLKVLIALGGERVAEELITDALWPRIDGDSAHRSFTSTLHRLRKLLGEDRALVLH
                     EGKLSLDARFFWVDAWAYERLLGELDSLLKPARERAAPELAERLGDRLLELYRGPFLA
                     GEAEEPWSLRPRERWHTRYARALDGLGRFWEASGQPARAREFRDRAQDAPRL"
     gene            8742..9206
                     /locus_tag="AC16O9_06290"
     CDS             8742..9206
                     /locus_tag="AC16O9_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516260.1"
                     /translation="MKGSRIAIAALASAALAGCYVVPGPDGQLWHVVPGHPGGPAAGG
                     PVPQGAGPALPVSLPVRLYPANEEATRTGVLTGTVVNMMTGKGRFQLNYQGETLSGEA
                     TRVDGDARKGVASAYGPTGMFMSCEYQMNSPRQGAGTCALSNGAKYQVHIGS"
     gene            complement(9185..>9412)
                     /locus_tag="AC16O9_06295"
     CDS             complement(9185..>9412)
                     /locus_tag="AC16O9_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516261.1"
                     /translation="QVLCRDVDCCTVHLRGGFDLLRSRLEGRKHRYMPASLLESQFAA
                     LEPPAGAIDIDVAQPVDACLAAIFSQLPMCT"
BASE COUNT         1376 a         3326 c         3339 g         1371 t
ORIGIN      
        1 cgcacgggtt cgaggcgcgc atgacgcgcg ccgaccacgc ctcgggcacc gaccgcatcg
       61 ccgaagccgc ggcgcagctc gggctggcgg atgacgcgat cgtcgtcaat gtgcagggag
      121 acgagccgct gatcgccccg cagctcatcg ccggcgtggc cgcggcgctc gccgcacggc
      181 ccgaagcgag cgtggccacc gcctgccatc cgatccacga cccggcggag ttcgccaacc
      241 cgaacgtcgt gaaggtggtg cgcgccgcga gcggcgacgc gctctatttc agccgcgccg
      301 gcatcccttt tccgcgcgac ggcggcacgc cggcggccga gcgccacatc ggcatctacg
      361 cttatcgcgt cgcgttcctg cgtcgctacg ccggcctcgc cccggcgccg ctcgagtcga
      421 ccgagcagct cgagcagctg cgcgtgctct ggcacggcca tcgcattacc gtggtgcagg
      481 cggaaggtgc catggcgccc ggcgtggata ccccggccga tctcgaagct gtgctaaaac
      541 tgctgcgatg agactcatcc tgctggggcc gccgggcgcg ggcaagggga cgcaggccgc
      601 tttcatcacc gagaagtacg gcatcccgca gatttccacc ggcgacatgc tgcgcgccgc
      661 ggccgaggcg ggcacgccgc tcggcctcgc ggcgaagaag ctgatggatg gagggcaact
      721 cgtccccgac gaggtagtcg tcgccctggt cgaagagcgc ctgcgcgcgc ccgattgcgc
      781 gcgcggctac ctgctcgacg gcttcccccg caccatcccg caggccgagg cgatgcgcaa
      841 ggcggcgctg ccgctcgagc gcgtcctgga gctcgacgtg cccgacgagg agatcgtcgc
      901 gcgcatgagc ggccggcgcg tgcacccggc ttccgggcgc agctaccacc agcagttcaa
      961 tccgccgcag acgcccgacc gcgacgatgt caccggcgag ccgctgatcc agcgcgagga
     1021 cgatcgcgag gcgacggtca ggaagcgcct cgaggtgtac cggcgccaga cgcgtccgct
     1081 ggtggagtac tacacccgct gggccgcctc gggcgacgcg cgcgcgccgc gcgtgcactg
     1141 catctcgggc tcgggcggca tcgaggaaat tcgcgacagg gctttcgccg cgctcggcta
     1201 gcctgataaa atcgccccct tttccgcggg gctcccagcc ccgcgcacgc gaaggggaga
     1261 aaaacatggc aggcggactc ggtttcgcgc tcatctgcgc actgttggcg atcgtctacg
     1321 gcgtctggca gagcgcgcag atcctcaggc tgcccgatgg caacgagaag atgcgccaga
     1381 tcgcggccgc gattcgcgaa ggcgccgtcg cctacctgtg gcgccagtac aagaccatcg
     1441 cgatggtcgg cgctgtgctg tttctcgcca tcggcgtcac gccgcagctc ggctggacca
     1501 cggcctgggg ctttctcttc ggcgccgtgc tctccggcgc ctgcggcatc atcggcatga
     1561 acatctcggt gcgcgccaac gtgcgcaccg cggaagccgc gcgcgtcggc ctgaacgagg
     1621 cgctgcaggt ggcgttcaag ggcggcgcca tcaccgggct gctggtcgtc ggactgggcc
     1681 tcgccggcgt ggcgggcctg tttgccctgc tctacgccaa tgcgctgaag aaagacgacc
     1741 tggtgcacat catccacccg ctgatcggct tcggcttcgg cggctcgctg atctcgatct
     1801 tcgcgcggct gggcggcggc atcttcacca agggcgcgga cgtcggcgcc gacctcgtcg
     1861 gcaaggtcga agccggcatc ccggaggacg atccgcgcaa ccccgctgtg atcgccgaca
     1921 acgtcggcga caacgtcggc gactgcgcgg gcatggcggc ggacctgttc gagacctacg
     1981 ccgtgaccat cgtcgccacc atgctgctcg gtgcgctcat gctgaagacc gaggcggcgg
     2041 tgatctaccc gctcgccatc ggcggcttcg ccatcatcgc ctcgatcatc ggcacctggt
     2101 tcgtgaaggc gaaaccgggc gaaaagaacg tgatgcccgc gctgtacaag gggctcgcgg
     2161 tcgccggcgg cgtcgcgctc atcgccttct ggccgatcac ccaggtggtg atgggcgacc
     2221 tgctgaagac cacgcagttc gacatcgcgg gcgcgaagac gacgatcggc gtctggcacc
     2281 tctacggcac cgccgtggtg ggcatggcgc tcacgggatt cctcgtctgg atcaccgagt
     2341 actacacggg cacgcagtac aagccggtgc agcacgtggc gcaagcttcc accaccggcc
     2401 acggcaccaa catcatcgcc ggtctgggcg tgtcgatgaa gtccacctcc tggccggtgc
     2461 tcgcggtgtg cgtcgccatc atcgcctcct atgcgctcgc cggcctgtac ggcatcgcca
     2521 tcgccgccac ctcgatgctc tccatggccg gcatcatcgt cgcgctcgac gcctatgggc
     2581 cgatcaccga caacgcgggc ggcatcgccg agatggccga cctgccggaa tcggtgcgcg
     2641 ccatcaccga tccgctggac gcggtcggca acaccaccaa ggcggtgacc aagggctacg
     2701 ccatcggctc ggcgggcctc gcggcgctgg tgctgttcgc cgactacacc cacgcgctcg
     2761 aggccgcggg caggtcggtg tccttcgacc tgtcgaacca catggtgatc gtgggcctct
     2821 ttatcggcgg cctggtgccg tatctcttcg gcgccatggc gatggaagcc gtgggccgcg
     2881 ccgcgggctc ggtagtggtg gaagtgcgcc gccagttcaa ggagatcccg ggcatcatgg
     2941 aaggcaaagc aaagcccgag tacggcgtcg cggtcgacat gctgacgcgc gcggcgatca
     3001 aggaaatgat gatcccgtcg ctgctgccag tgctggtgcc gatcgtcgtc ggcgtggtcc
     3061 tcgggccgca ggcgctcggc ggcgtcctga tgggcaccat cgtcacgggc ttgttcgtgg
     3121 ccatctccat gtgcacgggc ggcggcgcgt gggacaacgc caagaagtac atcgaggacg
     3181 gcaaccacgg gggcaagggc tccgaagcgc acaaggcggc ggtcaccggc gacaccgtgg
     3241 gcgaccccta caaggacacc gccggcccgg cggtcaaccc gctcatcaag atcatcaaca
     3301 tcgtggcgct gctcgtcgta cccttgatgg tgtgagcgtc cgcgaatcgc acctgcgaag
     3361 ggccgcgtgc gcggcccttt tctttttctg cgccggcgtc ggcgcgcagc tcgccgcgcc
     3421 ggaggtggca agcgagcgcg tcgagcgcga ggcagcctac gccaagcgtt tcatggtcgc
     3481 tgccgctgat ccgcgcgcgg tcgacgccgg cctggagatg cttcgtgcgg gcggcagcgc
     3541 tgccgacgcg gcgatcgccg cgcaactcgt gctcgggctc gtggagccgc agtcctcggg
     3601 cctgggcggc ggggcgttcg tgctgacgtg gtcggaaaag gacaagcgcg tgcgcagctt
     3661 cgacgcgcgc gaaatcgcgc ccgcgacggc gcgccccgac cggttcctcg accgccacgg
     3721 caatcccgag cagttcatgc acgcggtgtc gagcgggcgc tcggtcggcg tgcccggggt
     3781 gccgcgcctg ctggaagtcc tgcaccgcga gcacgggcgg ctcgcctggc cgcggctgtt
     3841 cgcgccggca atccggctcg ccgagcgggg gttcgagaag acgtcgcgcc ttgcgcgcca
     3901 gctcgagcgc gaagcgctgc tcggcgccga tcccgcggcg cgtgcgctgt tctacgcggg
     3961 cgagcgcatc gcgaatccgg actatgcggc gacgctgcgc gcgctcgccg gacagggcgc
     4021 cgacgcgttc taccgcggcg cgatcgccgc ggacatcgtg cgcgcggtta acgcggactt
     4081 ggcgctcgcc gacctcgccg gctaccgcgt catcgagcgt gacccggtgt gcgggcgcta
     4141 ccgcggctac cgcatctgcg gcatggggcc gccgagctcc ggcgccaccg gcgtgctgca
     4201 gatcctcggc attctcgagc gcacggcgtt cgagcgcgcg gcgcccggct cggccgaagc
     4261 ggtgcatcta ttcagcgagg ccgcgcgcct ggcgtacgcc gaccgcgcct tgtacgtagg
     4321 ggatcccgat ttcgcgccgg tgccggtctc gcgcctgctc gagccccggt acctcgctgc
     4381 gcgtgcgcgc ctgatcgggg agcgctcgat gggcttcgcg ttcccgggca ccctggatgc
     4441 ggagacgccg ggcaccagcc atctctcggt cgtggatggc gagggcaacg cggtcgcgat
     4501 gaccacctcc atcgagatgg gcttcggcag ccgcatcctg gtgcgcggct ttctcctcaa
     4561 caaccagctc accgatttca gcttccggcc ggaggcgctc ggccggcccg ccgtgaaccg
     4621 tgtcgaaggc ggcaagcggc cgaggagctc gatggcgccg acgctcgtct tcgagcgcga
     4681 cgggcgcctg aagatggcgc tcggctcgcc gggcggcctc gcgatcatca atttcgtggc
     4741 gaagacgctg gtggcggcgc tcgactgggg catggacatg caggcagcga tctcgtcgcc
     4801 gaacttcggc agcaccaacg ggccgacttt cgtggagcgc ggcaccgcct acgaggcgct
     4861 gggcgacgcg ctcgccgagc gcgggcatgt gctcaacttc gcgcggctga cgagcggctt
     4921 gcacgccatc gagcgcgtgc cgggcggctg gcgcggcggc gcggatccgc ggcgctcggg
     4981 caaggcggcg ggggactagg cagaaaaaac ccccggcgca cgagccgcgc gccgggggcg
     5041 atcgggggac cggagaggag gagggatacc ggccgcccca gttgcgactc cattcttccg
     5101 cgccgggccg ccgagcgcga ggaactatgt aacgctgccg ccgttggcct gatagtttct
     5161 tactttcggc agcgcgcggc gttgagcgct tgtaagccaa tcgtaagtgg tcggtgaccc
     5221 gcaagttatc ggccgccgcc tatcctcgtc gcatggacgg cacgcgccgt cggcggacga
     5281 ggaaaggagg caggccgatg ctgacgcccc gagaaagaga agtgctgcgc catatcgcgc
     5341 agggcctgag ctacgccgaa gtcggcaccg agctcggcgt gagccccaac acggttacca
     5401 gtcacgtgaa gaacgcctac cgcaagctcg acgtgcatac ggcggccgcg gccgtgatgc
     5461 gcgcggtaga gttgcggctg atcgggcagc agtagtcgtt gccgcaacgc agcacggctg
     5521 taagccaaaa gtaaccgcgt cccgcgcctg cgggagcaga atgcccttga ccgccatgaa
     5581 cgcccccgct tctcgcaggc gctcgccgct tggcaagacc acgcggccct tgctcgcgca
     5641 gattctgccg cgcgagcgcg tgttcgcgct gctcgacgag gcgagcgaca tgccgctcac
     5701 ctgggtgtcg ggcccgccgg gcagcggcaa gaccacggcg gtggcgagct ggctcgacca
     5761 cgcgccgcag cgcacgctgt ggtaccaagt cgacgagggc gacgccgacc cggcgacctt
     5821 cttctactac ctcggcctcg cggtcgccga cctcgagggc gccgagcacc ccgcgctgcc
     5881 gctgctcacg cccgagtacc acgccgggtt ggtcgcgttc acgcgccgct acgcgcagga
     5941 gttgtacgcg cgcctgaagc cgccgttcgt catcgtcttc gacggctacc acgaggtccc
     6001 cgcgtcctcg ccgctgcacg aggcgatgcg cgaagcgctg cgcgagctgc cgccaggcgg
     6061 ccgcgccatc gtcgtcagcc gcgccgaccc gccggcggcg ctggcgcggc tgcgcgccaa
     6121 ccagcagctc gcgcacgtcg gctgggagga gctgcgcctg acgcgcgaag agacagtgag
     6181 cgtggctgcg cagcgccgcc cggagctcgc ggccgatgcg ctcgcgggcc tctacgacaa
     6241 gacgcagggc tgggcggcgg ggctggtgct gctgctgcag cacgcgcgca tgggggcgcc
     6301 gatcgtcgac gcgcccgact tcgccacgcg ccagctggtg ttcgactacc tggcgggcga
     6361 aatcctgcag aagtctgacg cgcgcacgca ggagttcctg ctgcgcaccg cgcacctgcc
     6421 gcagatgacc gccgcgctgg cgcagcagct gtccggcgag gcggcggccg gcgagatgct
     6481 cgcgcggctg caccgcgaga actactttgt cgcgctgcgc gaagcgcacg ccgagcccgt
     6541 gtaccagtac cacccgatgt tccgcgagtt cctgctggcg cgcgcacagg aggcctatcc
     6601 caaggaccgt cgccgccagc tgcagaagga cgccgcggcc ctgctggagg gcgccggcca
     6661 gatcgaggag gcggtggcgc tcgcgcgcga cgcgcacgac tgggacgcga tggcgcggct
     6721 catcgagcgc catgcgggcg cgatgttcgc ccagggtcgc gccgagaccc tagtgcattg
     6781 ggtcgaggag ctgccgcccg aagtgcagca ggcgcatccc tggaccgtgt actgggcggc
     6841 cgccagccag gcgcagcgcg cgccgcgtga agggcgcctc ggcttcgagc gtgcctacga
     6901 gttgttccgc accgccgccg cgcccgactc ggcgggcagg gtactcgcct gctccggggc
     6961 gatggacgcg atcctgtacg aactggacga tttttcgctg ctcgaccgct ggatcgcggt
     7021 gctcgacgcc atggcgctcg agggcatgcg cctgcccacg ccggaggccg aggcgcgcgt
     7081 cgcctgcagc atgttcatcg cgctcacgct gcgccagccg cagcgccgcg acatcgagca
     7141 atggatccag cgcgccatcg ccgcctcgca gcaggtgccc gacaccaacc tgaggatgtt
     7201 cgtcggcctg ctcgcggcgc tgacgctgat gtggacgggc gtattggtgc gcgccgacgg
     7261 cctgatcgag gccatgcgca agctcgccgc gacgccgggc gtctcgccgt tctcactgct
     7321 cacgctgaag aacgtcgagg cgatgtacca catgctgacg gccgcgcccg agccctgcct
     7381 cgccgccatg cgcgagggcc aggagatcgc gcgcgccacc ggcatccata cctggagcct
     7441 gcagctgctg gtcaacggtt atggcggcgc gctcggcggc ggcgacctcg cggccgcggc
     7501 gccgctcgcg cgccagctcg agtcgcaggc gagcggcgcc ggccgcttca acctgtgcct
     7561 gtaccaccat ttccaggcct gggaagcggc gctgcgcaag gacctgatgc gcgcgcacga
     7621 gcacgagcgc cgtgcgctgc gcatggcgat cgaggtaggt tgtccgtttc tcgaggtgct
     7681 gacgcgcctg gcgctggcgc aggtgctcgc cgaatgcggc gacgaaagaa agtgcatttc
     7741 gcacctgacg cagatgcgcg ccatcgcgcg caccatcgat agccaccacc tcgagtacac
     7801 ctgcctgctc ggcttcgcgg gcatcgcgct cgagcacgga cgcagccgcc cgggcctgaa
     7861 cgcgctgcgc cgcggcttcg cgctcgggcg cgagcacaac taccggcact tcctgtggtg
     7921 gcggccgact gccatcgcgc gcttgtgcga gcacgcgctc gccgccggca tcgagaccga
     7981 gtacgcgaag agcctggtca agcgccgcgc gctcgccccc gagcgcccgc cgctcgacgt
     8041 gcccgagtgg ccatggcatt ttcgcgtgca cacgctgggg ggctttcgcc tgctgcgcca
     8101 cggcgagccg ctcgcggcgg cgaccggcaa ggcgcagcgc cggccgctcg agctgctcaa
     8161 ggtgctgatc gcgctgggcg gcgagcgcgt cgccgaggag ctgatcaccg acgccttgtg
     8221 gccgcgcatc gacggcgact cggcgcaccg ctcgttcacc agcacgctgc accggctgag
     8281 gaagctcctg ggcgaggatc gcgcgctggt gttgcacgag ggcaagctct cgctcgacgc
     8341 gcgcttcttc tgggtcgatg cctgggccta cgaacggctg ctcggcgagc tggactcgtt
     8401 gctcaagccg gcgcgcgagc gcgccgcgcc cgagcttgcc gagcggctgg gcgaccggct
     8461 gctcgagctc taccgcggcc cgttcctcgc tggcgaggcc gaggagccct ggagcctgcg
     8521 cccgcgcgag cgctggcaca cgcgctatgc gcgcgcgctc gacggcctcg ggcgcttctg
     8581 ggaagcatcg ggccagccgg cgcgcgcgcg tgagttccgc gaccgcgccc aggatgcgcc
     8641 gcgcctgtga gcgatcggta accgagcggt aacccgcgcg tatctggtct gcgcccatca
     8701 tggtcacgca ggcgcttgcc tgcgcaccag acggagggat catgaaaggt tccaggatcg
     8761 caatcgcggc gcttgcttcg gcggcgctgg ccggctgcta cgtggtgccc gggccggacg
     8821 ggcagctctg gcacgtcgtg ccgggccatc ccggcggacc ggccgcgggc gggcccgtgc
     8881 cgcagggggc cggccccgcg ctgccggtgt cgcttcccgt gcgcctatat ccggcgaacg
     8941 aggaagccac tcgcaccggc gtactcaccg gcacggtggt caacatgatg acgggcaaag
     9001 ggcgcttcca gctcaactat caaggcgaga cgctctccgg cgaggcgacg cgcgtcgacg
     9061 gcgacgcgcg caagggcgtg gcgagcgcct acggtccgac cggcatgttc atgagctgcg
     9121 agtaccagat gaactcgccg cgccagggcg ccggtacctg cgcgctgtca aacggcgcca
     9181 agtatcaggt gcacatcggc agctgagaga atatcgccgc caagcacgcg tccaccggct
     9241 gcgcgacgtc gatgtcgatg gcgccggccg gtggctcgag ggcggcgaac tggctctcga
     9301 gaagcgacgc cggcatgtag cggtgcttgc gcccttcgag gcgcgagcgc aggaggtcga
     9361 agccgccgcg caggtggacg gtgcagcaat cgacgtccct gcacaggacc tg
//
LOCUS       DBNHKE010000102         5410 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_652015, whole genome shotgun sequence.
ACCESSION   DBNHKE010000102 DBNHKE010000000
VERSION     DBNHKE010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_652015"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="AC16O9_06300"
     CDS             complement(<1..619)
                     /locus_tag="AC16O9_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6516262.1"
                     /translation="MRGAALLRIAAAALLLPLASLASSPEPALSGVVEAIEANRLDLA
                     LERVEKLIATHPNFRLAHLIRGDLLLARARPLQTFGNVVKTVPREKVEDLRAEALARL
                     RALREERPHAERLPRAILQLRPDQRHALLVDSRHSRLYVFENVDGRPQYVADYYVSLG
                     KRGVDKAREGDQKTPIGVYHVTANLPRQKLTDFYGAGAYPINYPNV"
     gene            complement(619..1614)
                     /locus_tag="AC16O9_06305"
     CDS             complement(619..1614)
                     /locus_tag="AC16O9_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6516263.1"
                     /translation="MHVLSAPRLSLAALAAAIILAGTPARADELAEASKLLRAGQHQQ
                     ALERVNKALAAKPADPQGRFLKGVIYAEQGNAGAAVEIFRKLTEDYPELPEPYNNLAV
                     VYASQGQYDKARAALEQSIRTHPSYATAYENLGDVYAKLASQAYDKALKLDSTNAGAQ
                     NKLALVRELVGSAPSEKSAPLVTATASPAPKAAPAPEVPPKPAPAKPAPKAAAPAAAP
                     GKDVLEAVAAWADAWSRQDAEAYLAAYAKDFRTPKGEPRDAWEKQRRARIAAPKSIVV
                     KVDNEQVSPAGDGRVRVTFRQDYQSDVFSGSATKTLLMGKSADGRWRILQEQVSN"
     gene            1714..3078
                     /gene="cysS"
                     /locus_tag="AC16O9_06310"
     CDS             1714..3078
                     /gene="cysS"
                     /locus_tag="AC16O9_06310"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR6516264.1"
                     /translation="MLRIFNSLARQKQDFTPIRPGEARMYVCGMTVYDYCHLGHARVM
                     VVFDVVRRWLRAGGYRVTYVRNITDIDDKIIRRAAEHGEAVADVTARFIRAMDEDLAA
                     LGVEKPDHEPRATQYVPQMLELIERLEQRRLAYRSSSGDVNFAVRRFSGYGKLSGKSL
                     DELRAGERVEVDASKDDPLDFVLWKRAKAGEPAWESRWGPGRPGWHIECSAMSCALLG
                     EHFDIHGGGQDLQFPHHENEIAQSEGASGRPFVNIWMHNGFVRVDEEKMSKSLGNFFL
                     IRDVLQRFDAEVVRFFILRAHYRSPLNYSDAHLEDAKQALTRLYTALKGAPASVGPID
                     WGEPRAQRFKAAMDDDFGTPEAVAVLFELANEVNRGRAELAGQLRALGGVLGILQREA
                     GEFLRAGVAGNVDSAWIEERIAARLAARKAKDFALADRIRAELEGFGIILEDGPQGTA
                     WRRA"
     gene            complement(3085..4218)
                     /locus_tag="AC16O9_06315"
     CDS             complement(3085..4218)
                     /locus_tag="AC16O9_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516265.1"
                     /translation="MIAPAPLLLAALALGLLAAPAWSQGVTARTVVLGQSAPLTGALQ
                     AQGEDIRNGALAYFRPLNDAGGVHGRRIELATLDDAGTPERALANTRRFIEEFKVFAL
                     FGYADVGTTRKLLTLAQQARVPLVGPVTGAALARQAGRSVYTVRAGHAAEIESVIGHY
                     AQLGLKRFALLRAEDAAGEEYLAAARSALSRRGLDAPMDAALRQGADGIAGLSREAFV
                     ADPDVVIFALPQPPAADLVRALMRTGKRTQLVALSPADPEQLAQALGADGAGVVLSQV
                     IPPLVQESLPVVAEYRAAIEAETGRKAYSPASFEAYIAAKVFAEAVRRVGPTLTRDAL
                     LLALDAMAYHDVGGHTVRFSRTRRQGSERIYLLALTRDGKLLH"
     gene            4256..4918
                     /locus_tag="AC16O9_06320"
     CDS             4256..4918
                     /locus_tag="AC16O9_06320"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HIR6516266.1"
                     /translation="MLLQIPGVLNRDEVATVMRELGEGAYEDGRGTAGAVARELKNNL
                     QLKSTAEAAKKCAPLVLAALRRNATFYNLALPHRIHGPIFNRYDVGMTYGSHVDNAIM
                     GDEVRSDVSATLFLSAPDSYDGGELVLQEHQSERRIKLPPGAMVVYGSTNVHRVEPVR
                     RGTRVAAVFWIQSLVREESKREILYDLSVIMNGLRDRLDAAENAAIAAVYSNLVRQWA
                     ES"
     gene            complement(5181..>5410)
                     /locus_tag="AC16O9_06325"
     CDS             complement(5181..>5410)
                     /locus_tag="AC16O9_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516267.1"
                     /translation="SVTNMRATLAGQPSFSGGGGTVAEVGLVPQSYSCASTNCIASFQ
                     GAFFGAGASHAGIAYQITSGQTVSGVAVFKQ"
BASE COUNT          850 a         1778 c         1920 g          862 t
ORIGIN      
        1 acacattggg gtaattgatc ggataagcgc cggccccgta gaagtcggtg agcttctggc
       61 gcggcaggtt ggccgtcacg tggtagacgc cgatgggggt cttctggtcg ccttcgcgcg
      121 ccttgtcgac gccgcgcttg cccagcgaga catagtagtc ggcgacatac tgcggtcggc
      181 catcgacgtt ctcgaatacg tagagacgcg aatgccgcga atcgaccagc agcgcgtgcc
      241 tctggtccgg tcgcagctgc aggatggcgc gcggcaggcg ctcggcgtgc ggccgctcct
      301 cgcgcagggc gcgcaggcgg gcgagcgctt cggcgcgcag gtcctccacc ttctcgcgcg
      361 gcacggtctt caccacgttg ccgaaggtct gcaggggccg cgcacgcgcg agcagcaggt
      421 cgccgcggat caggtgcgcg agtcggaagt tcgggtgcgt ggcgatcagc ttctcgaccc
      481 gctccagcgc gaggtccagg cggttggcct cgatcgcctc gaccacgccg gacagggcag
      541 gctcgggcga tgaggcgagg ctcgccagcg gcagcaggag cgccgcggcg gcgatgcgca
      601 ggagcgccgc gccgcgcatc agtttgacac ctgctcctgc aggatgcgcc agcgcccgtc
      661 cgcggacttg cccatcagca gcgtcttggt ggcgctgccg ctgaacacgt cggactggta
      721 gtcctggcgg aaggtgacgc gcacgcgtcc atcgccggcc ggactcacct gctcgttatc
      781 gaccttcacg acgatcgact tgggcgcggc gatacgcgcg cgccgctgct tctcccatgc
      841 gtcgcgcggc tcgcccttcg gcgtgcggaa gtccttggcg taggccgcca ggtaggcctc
      901 ggcatcctgg cgcgaccagg cgtcggccca ggccgccacc gcttcgagca cgtctttgcc
      961 gggtgcggcg gccggcgcgg ctgccttggg cgccggcttg gccggcgcgg gcttgggcgg
     1021 tacttccggc gccggtgccg ccttcggcgc cgggctggcg gtggcagtta ccaggggggc
     1081 cgacttctcg ctcggcgcag acccgaccag ttcgcgcacc agggccagct tgttctgcgc
     1141 cccggcattc gtcgaatcga gcttcagtgc cttgtcgtag gcctggcttg ccagcttggc
     1201 atacacgtcg cccaggttct cgtaagccgt ggcatagctc ggatgcgtgc ggatcgattg
     1261 ctcgagcgcc gcgcgcgcct tgtcgtactg gccctgcgac gcatagacca cggccaggtt
     1321 gttgtacggc tcgggcagct ccgggtaatc ctcggtgagc ttgcggaaga tctcgaccgc
     1381 cgccccggcg ttgccctgct cggcgtagat gacacctttc aggaagcggc cctgtggatc
     1441 ggcgggcttg gccgcgagcg ccttgttgac gcgctccaag gcctgctggt gctggcccgc
     1501 gcgcaacagc ttgctcgcct cggcgagctc gtcggcccgt gcaggcgtgc ccgccaggat
     1561 gattgcggcg gcgagcgccg cgaggctcag tcggggcgcg ctcaagacat gcattgtgtt
     1621 atagttttca aggatatacg gaaacttgct gattctagac taagccccgc gggatcacaa
     1681 ccgagcggcg gccaagccac gcacagcccc tcgatgctcc gaatcttcaa ctcgctggcg
     1741 cggcaaaaac aggacttcac gccaatccgt ccaggcgaag cgcgcatgta cgtatgcggg
     1801 atgacggtgt acgactattg tcacctcggc catgcccgcg tgatggtggt attcgacgtc
     1861 gtgcgccggt ggctgcgcgc tggcggctac cgcgtcacct acgtacgcaa catcaccgac
     1921 atcgacgaca agatcatccg gcgcgcagcg gaacatgggg aggcggtcgc cgacgtgacc
     1981 gcgcgcttca tccgcgccat ggatgaggac ctcgccgcgc tcggcgtgga aaagcccgac
     2041 cacgagccgc gcgccacgca gtacgtgccg caaatgctcg agctgatcga gcgcctcgag
     2101 cagcggcgcc ttgcttaccg ctcatcctcc ggcgacgtga acttcgccgt gcggcgcttt
     2161 tcgggctacg gcaagctctc gggcaagagc ctcgacgagc tgcgcgccgg cgagcgcgtc
     2221 gaggtcgacg cgtccaagga cgatccgctc gacttcgtgc tgtggaagcg cgcgaaagcc
     2281 ggcgaaccgg cgtgggaatc gcgctgggga ccgggccggc ccggctggca catcgagtgc
     2341 tcggcgatga gctgcgcgct gctcggcgag catttcgaca tccacggcgg cggccaggac
     2401 ctgcagtttc cgcaccacga gaacgagatt gcgcaatccg agggcgcgag cggacggccg
     2461 ttcgtcaaca tctggatgca caacgggttc gtgcgcgtgg acgaggaaaa gatgtccaag
     2521 tcgctgggca acttcttcct catccgcgat gtgctgcagc gcttcgacgc cgaggtggtg
     2581 cggttcttca ttttgcgcgc ccactaccgc agtccgctga actattccga tgcgcacctc
     2641 gaggatgcga agcaggcgct gacgcggctg tacacggcgc tgaagggcgc gcccgcatcg
     2701 gtcggcccca tcgactgggg tgagccgcgc gcgcagcgct tcaaggcggc gatggacgac
     2761 gatttcggca cgccggaggc ggtcgcggtg ctgttcgagc tcgccaacga ggtgaatcga
     2821 ggccgcgcgg agctcgccgg gcagctgcgc gcgctgggcg gagtgctcgg catcctgcaa
     2881 cgcgaggccg gggagttcct gcgggcgggg gtggccggca acgtggactc ggcatggatc
     2941 gaggagcgca ttgcggcgcg cctcgcggcg cgcaaggcga aggactttgc tctggccgac
     3001 cgcatccgcg cggagctcga gggctttggc atcatcctgg aggacggtcc gcagggcacg
     3061 gcctggcggc gcgcctagcg ctgctcagtg cagcagcttg ccgtcgcgcg tgagcgccag
     3121 caggtagatg cgctcgctcc cctggcggcg ggtgcgcgag aatctcacgg tatggccacc
     3181 gacatcgtga taggccatcg cgtcgagcgc cagcagcagc gcgtcgcgcg tcagcgtcgg
     3241 gccgacgcgg cgcacggcct cggcgaacac cttggcggcg atataggcct cgaacgaagc
     3301 aggtgagtac gccttgcgcc cggtctcggc ttcgatcgcg gcgcggtatt ccgcgacgac
     3361 ggggagcgac tcctgcacca gcggcggtat cacttgcgaa agcacgacgc cggcgccgtc
     3421 ggcgccgagt gcctgggcaa gctgctcggg gtcggccggg gacagcgcga ccagctgcgt
     3481 gcgcttgccc gtacgcatca gcgcacgcac caagtccgcc gccggcggct gcggcagcgc
     3541 gaagatgacg acgtcgggat cggcgacgaa cgcttcgcgc gacaggccgg cgatgccatc
     3601 cgcaccctgc cttagtgccg cgtccatggg ggcgtcaagg ccgcggcgcg acaacgccga
     3661 gcgtgctgcc gcgaggtact cctcgccggc agcgtcctcc gcacgcagca gggcgaagcg
     3721 cttcaggcca agctgcgcat agtggccgat gacgctctcg atttcggcgg cgtggccagc
     3781 gcgcaccgtg tagacgctgc gtccggcctg gcgcgccagc gcggcgcccg ttacaggtcc
     3841 gaccaacggc acgcgcgcct gctgcgccag cgtcagcagc ttgcgcgtcg tgccaacgtc
     3901 ggcgtagccg aacagcgcga agaccttgaa ctcctcgatg aagcgacgcg tgttggcgag
     3961 cgcgcgctcg ggcgtgcccg catcgtcgag cgtcgcgagc tcgatgcgcc gcccgtgcac
     4021 gccgccggcg tcgttcagcg gacggaagta ggcgagcgca ccattgcgga tgtcctcgcc
     4081 ctgggcttgc agcgccccgg tgagcggcgc cgactggccg aggaccacgg tgcgcgcagt
     4141 gacgccctgg ctccaggccg gcgcggcgag gagcccgagt gccagcgcgg cgagcagcaa
     4201 gggcgcgggt gctatcatcg agcgcaattt tccacgattg tcggacttgg cgcgcatgct
     4261 gctgcagatt cccggggtgc tgaataggga cgaggtcgcg acggtgatgc gcgaacttgg
     4321 cgaaggagca tacgaggacg ggcgcgggac cgcgggcgcc gtcgcgcgcg agctgaaaaa
     4381 caacctgcag ctcaagagca ccgccgaggc cgcgaagaag tgcgccccgc tcgtcctggc
     4441 ggcactgcgt cggaatgcga ccttctacaa cctggcgctg ccgcaccgca ttcatgggcc
     4501 gatcttcaac cgctacgatg tcggcatgac gtacggctcg cacgtggaca acgccatcat
     4561 gggcgacgag gtgcgatcgg acgtttcggc gacgctgttc ctcagcgccc ccgattccta
     4621 tgacggcggt gagctggtcc tgcaggagca ccagtcggag aggcgcatca agctaccccc
     4681 aggcgccatg gtggtgtacg gctcgaccaa tgtccaccgg gtcgagccag tacggcgcgg
     4741 cacacgggtc gcagcggtgt tctggattca gagcttggtg cgcgaggaat cgaaacgcga
     4801 gattctctac gacctgagcg tcatcatgaa tgggctgcgc gacaggctgg atgccgcgga
     4861 gaacgcggcg atcgcggctg tctacagtaa cctggtgcgg cagtgggcgg agagctaggc
     4921 gggtcggcgc tgcggcgtgg ccttttgaac agcagtatca ggtctgcgcc tggctccggc
     4981 gaggatgctc caagcgcgac ggcgcgcagc acgctctgta tggtgccccg agaattgccg
     5041 gtgcgctcgt ggcactggcc cgcaagccgg atcgtccgtg gatcgtccgg cgccgccttg
     5101 gggcggcggc cccaaacgga catagcgtcc cgcagccggc cggctgcggg atgcaggagg
     5161 acaacagcgg cgcccggtgc ttactgctta aagactgcca cgccggacac cgtctggccg
     5221 ctggtaattt ggtacgcaat cccagcgtgc gaggcgccgg ctccgaagaa ggctccctga
     5281 aagctggcta tacagttggt ggaagcacat gaatagcttt gagggaccag gccgacctca
     5341 gctacggttc caccgccacc ggaaaaggat ggctgacctg ctagcgtggc gcgcatattt
     5401 gtcacgctgt
//
LOCUS       DBNHKE010000103        18974 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_862435, whole genome shotgun sequence.
ACCESSION   DBNHKE010000103 DBNHKE010000000
VERSION     DBNHKE010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_862435"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2308
                     /locus_tag="AC16O9_06330"
     CDS             <1..2308
                     /locus_tag="AC16O9_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6516268.1"
                     /translation="REKAHAAERARREAAEQARREAEELRFKIEEERLAREEAERRAR
                     AEAERTKREAAERARREEEERKARAEAEELRKKYEEERKAREEAERRAEEERQRREQQ
                     RLEEERKAREEFERQEAERRAADEAERRAREAEERKEAEEHQRQIRLAREAEDKKRRD
                     AEARKKKEAEAKRWEVEEARRRADDAYRQRAEQEDKERAEREAVEAAERAEADKRAAA
                     EADALAGSLLADLDSFSSRQDEERKTKEEQERQERAEAERLAAQERAQREREEAERKA
                     KKTEERRRREEEEQREREAKEQREREEEARRKEEEAERKRKERELAARNKEVTDIPIS
                     SADLDRGDVEDDERKLTPEARKALRDREREVARARSRGDVSGKKAAAAYKRKQPTQWG
                     KPVAIFLFLTLVISAGVANFIPLDTATYEKAASDAVGAPVKIGAARLWVLTGVQLRFE
                     RVTVGDAVKVAEIRAVPEFASLLGDERIFSRLEFNRAVVQQGALGNALFGTMKQGGLK
                     VARVVAKDLRFEGPIALPRMDIDAAVGPDGRVSSVRLSGERVNGLLTLREDRVGFEVT
                     MGSFTVPFVDKLTLAEFGMKGSAESGGLSADFDGRLYDGTVVGKARVEWGAQWSVQGE
                     IQGRTMNAAVFAPQMVSEGRFDGKGRFAMSGAEPAQLDDSARLDGDFTVTKGALGSFD
                     LSRALQSASAQTSGRTPFSELEGRASLASGALAFRDLKLTAGLLNARGALDVDAKGGL
                     SGRIDAELRNLRSTLYIGGRLSDPQLRR"
     gene            2308..6303
                     /gene="purL"
                     /locus_tag="AC16O9_06335"
                     /gene_synonym="purI"
     CDS             2308..6303
                     /gene="purL"
                     /locus_tag="AC16O9_06335"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR6516269.1"
                     /translation="MLLQLRGARALSDFRLAKLLPLLQRAAPGVRAVRAEFRHFVELD
                     GAPSAQDEALLARLLRYGAAPLEPGGGDTLYLVVPRIGTLSPWASKATDIARNCGLTQ
                     VRRIERGTAYYVAGAARSAAVAALLHDRMTETVLGAFEEAAQLFRHVPPRPLGHVALA
                     ALDEANARLGLALSGDEIQYLRQAYAALGRDPTDAELTMFAQANSEHCRHKIFNADWV
                     VDGERQPRSLFAMIRHTHAVQPRGTVLAYADNAAIMEGRLAQRFYAGADGRYAAHAEP
                     THTVMKVETHNHPTAISPFPGAATGAGGEIRDEGATGRGAKPKAGLVGYSVSNLRIPG
                     SERAWEDDYGKPGRIVSALSIMLEGPIGAAAFNNEFGRPNLAGYFRTFEAGVGGVRRG
                     YHKPIMLAGGVGNIAARDSAKAPLPEGSLLIQLGGPGMLIGMGGGAASSMGAGSNVED
                     LDFDSVQRGNAEIQRRAQEVIDACWALGQGNPILSIHDVGAGGLSNALPELAHGAGRG
                     ARLDLRAAPSEDSGMSPREIWCNEAQERYVLAIAPSSLERWRAICARERCPFAVLGTA
                     TADGRLRVEDPQLADTPVDVPLEVILGKPPKMLRDVKRLARRGDAFALDGVVLKDAAY
                     RVLRHPAVADKTFLIAIGDRTVGGLCSRDPFVGPWQVPVADCAVTLIDFDGVAGEAFA
                     VGERTPLASVNAPASGRMAVGEALTNLAAARIESIERVKLSANWMAAAGYPGEDAALF
                     DTVQAVAMELCPALGVSIPVGKDSLSMRTAWEGKEVVAPVSLVVSAFAPVEDARGTLT
                     PRLRTDAGETELVLVDLGRGKNRLGGSILAQVHAKFGNEVPDLDDPALLKGFFGAIQK
                     LAAERLVLAYHDRSDGGLFATVCEMAFAGHCGATLNLDAIAFDALTEDVDGFRKSGEE
                     QLSGRVREQILKCLFSEELGAVLQIRAADRAKVMDTLRAAGLGPVSHLIGTLNDRDEI
                     RFTRNAKPVFAEPRVELQRAWSETTYLLQKLRDNPECAQEEYDRILDAGDPGLHVALT
                     FDPKEVFVNTGAAPRIAVLREQGVNGQVEMAAAFTRAGFEAIDVHMSDLISGRLSLDS
                     FKGFAACGGFSYGDVLGGGQGWAKSILFNSRARKAFEAFFGRSDTFALGICNGCQMMS
                     ALKELVPGAGHWPSFERNRSEQFEARLVMVEIVDSPSIVFKGMAGSRIPGVTAHGEGR
                     AQFASETDRKKAIVAARFVDNRGKVTETYPLNPNGSPEGITGLTTADGRFTILMEHPE
                     RVFRTVQMSWAPDEAGEDSPWMRMFRNARGWVGSGWDSCFEMAVGRVYAGGYMPAYGG
                     IYRQMVQSVYR"
     gene            6565..6888
                     /locus_tag="AC16O9_06340"
     CDS             6565..6888
                     /locus_tag="AC16O9_06340"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6516270.1"
                     /translation="MTQPVYALNLFNVADKDEYLAYSRRSHKEVMAHGGKVVALGKFR
                     EAAAGSIEPRAVMILVEWASKAAFESYRDDPKLADLHPHRVNGTRDYIWHLFDKLEDL
                     RPILR"
     gene            7039..7431
                     /locus_tag="AC16O9_06345"
     CDS             7039..7431
                     /locus_tag="AC16O9_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6516271.1"
                     /translation="MTLITHRGGCHCGRVRFEVDAPAHIEVTDCNCSICAKTAYLHLI
                     VPRARFRLLSGEDALTTYEFNTRTAKHLFCSTCGIKAFYVPRSHPDGVSVNARCLDEG
                     TVTGMSIRPTDGRNWEQAYPEGRGAFLE"
     gene            7495..7788
                     /locus_tag="AC16O9_06350"
     CDS             7495..7788
                     /locus_tag="AC16O9_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6516272.1"
                     /translation="MPTFIALANFTDQGVKTAKDSVNRAEAVKAMGEKYGVKLKDIYW
                     TMGAYDVVAIFEAPDGPTMSAVSLAVAAAGNIRSQTLRAYTKEEMKEVLKKLP"
     gene            7788..8003
                     /locus_tag="AC16O9_06355"
     CDS             7788..8003
                     /locus_tag="AC16O9_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516273.1"
                     /translation="MAETRPDPDVAVDREAIDRHVRKLVAEKARRDAEKFERLRRRPL
                     SVAETLALVLAAAAVVTVGALALIALR"
     gene            8033..8305
                     /locus_tag="AC16O9_06360"
     CDS             8033..8305
                     /locus_tag="AC16O9_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516274.1"
                     /translation="MSDPEHQARLARLRELLQIPDRDRTDEQWDELNELEIVLASSNR
                     QGAPRPEQQGQGGQNRPRHGGGPHPGNPGRKFHNRPPKRRGPPPGR"
     gene            8341..8688
                     /locus_tag="AC16O9_06365"
     CDS             8341..8688
                     /locus_tag="AC16O9_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6516275.1"
                     /translation="MAITRHDPGPLLSLAVEHGDTVYLAGIVAKNLKNDVKGQTEEVL
                     AEIDRLLAKCGSNKSKILQATIWVPDIRLRTAMNEVWTKWVDPKNLPGRACIEAKLAD
                     PAALVEIMVTAAK"
     gene            complement(8711..8938)
                     /locus_tag="AC16O9_06370"
     CDS             complement(8711..8938)
                     /locus_tag="AC16O9_06370"
                     /inference="COORDINATES: protein motif:HMM:NF018369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HIR6516276.1"
                     /translation="MTKTALALALALAVSPALAQRKDCNELKSEIDAKITANGVKVFT
                     TTIVDKDAPEDGKVVGTCDGGTKKIVYKRGG"
     gene            complement(9144..9950)
                     /locus_tag="AC16O9_06375"
     CDS             complement(9144..9950)
                     /locus_tag="AC16O9_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6516277.1"
                     /translation="MAMPRLGQGTWNMGDSASAREDELAALRAGIELGMTLIDTAEMY
                     GNGRSESLVAQAIAGIRDRIFLVSKVLPSNASRKGVVRACEASLKRLKTDCLDLYLLH
                     WSGSHPLEDTVRGFEELLAAGKIRSWGVSNLDLGEMNQLVAVPGGKACVTNQLLYNLA
                     RRGIEFDLLPWCRERKISVMAYSPVEQGRILGHKALKEVAQRLNATPAQVALAWVLRQ
                     DGVVAIPKAASVAHVRENHKALALKLDDAALKACDAAFPPPQKARPLEML"
     gene            complement(9959..11323)
                     /locus_tag="AC16O9_06380"
     CDS             complement(9959..11323)
                     /locus_tag="AC16O9_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR6516278.1"
                     /translation="MADSVKYILDETRIPKRWYNIAADLPKPLPPVLHPGTKQPIGPD
                     DLAPLFPMALIGQEVSTEREIEIPEPVRDIYKQWRPTPLYRARRLEHALQTPARIYYK
                     YEGVSPAGSHKPNTAVAQAFYNKEAGVKKITTETGAGQWGSSLAFAGALFGIEIKVFM
                     VRVSYEQKPYRRAMMETYGAKCIASPSPETNSGRAILAQRKDHPGSLGIAISEAVEVA
                     ATNDDTKYALGSVLNHVLTHQTVVGQEAMAQMEMAGDYPEILIGCTGGGSNFAGLVFP
                     FLGAQLRGGKKVRVIAVEPAACPSLTRGKYAYDFGDTGHLTPLVKMHTLGSTFTPPSF
                     HAGGLRYHGMSPLVSHCQELGLLESRAYAQTEVFSAGVQFARAEGIIPAPEANHAVKA
                     AIDEALRCKTEGRSRVILFNLCGHGHFDMQAYTDYFSGKLTDQKYDEKELAMALAGLP
                     SVAA"
     gene            complement(11388..12221)
                     /gene="serB"
                     /locus_tag="AC16O9_06385"
     CDS             complement(11388..12221)
                     /gene="serB"
                     /locus_tag="AC16O9_06385"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR6516279.1"
                     /translation="MNLVLQGPGASAAHAAEAARLCGAREIEQLADAAFRLLGAGRNA
                     AVIEWCHEHKLDCAWVPPGRRMADLRLVAMDMDSTLVTIESIDEMGEMLGIKDRVAQI
                     TAQAMSGEIDYAESLRRRVGLLAGLEEAAMERIWAQRMHLSPGAEALVRRCRERGIRT
                     LLVSGGFDFFTSRLQRRLGIDAALANALEIERGRLTGRVLGPIVDGRAKAEKLREQMR
                     RLGLRPEQALAIGDGANDIPMMSVAGTSIAYRAKPAVRARATHALDHAGLDGALHLFA
                     D"
     gene            complement(12218..15601)
                     /gene="mfd"
                     /locus_tag="AC16O9_06390"
     CDS             complement(12218..15601)
                     /gene="mfd"
                     /locus_tag="AC16O9_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR6516280.1"
                     /translation="MLRDPLPGSAHPTGLAGSADALALARLGTSRAPLAVITSSARDA
                     QRLVEELAWFAPQLRVCLLPDWETLPYDAFSPHQDLVSERLATLYRIQRGEFDVAVVP
                     AQTALVRLCPPSYLAARTFFLKQDTRLDAEALKAQLATAGYQHVTQVVAPGEFCIRGG
                     LIDLFPTGSQLPYRLDLDDERIDSIRTFDVDTQRTLYPVQEVRMLPAREFPLDEAGRA
                     KFRSRWREVFEGDPSKKGLYRDVSNGVPAAGVEYYLPLFFDEVATLFDYLPAHCALAR
                     HGDVPGAVDAFWRDAASRYKLLGGEHERPLLPPATIFVPAEEFHVAARARGRVEPESV
                     LASAPLPPLAVDRRAQDPLAGFKRFLESSALRVLVAAESPGRRETMLAYFAEYGLAPV
                     ALADFAAWSRSPERFALTVAPLNAGFALPAEGWCVVTEAELYAGTVRQRARDRQKQSS
                     VEGMLRDLSELRIGDPVVHAQHGIGRYQGLVGLDLGEGPTEFLHLQYDGGDKLYVPVS
                     QLAVISRYSGAEAEAAPLHKLGSGQWDKAKARAAKQVRDTAAELLALYARRAAREGHA
                     FKLQAHDLDAFAAGFGFEETPDQAAAIQAVVADMAAAKPMDRLICGDVGFGKTEVALR
                     AAFVAVADGKQVALLCPTTLLAEQHFQTFSDRFADWPVRIAELSRFRTAKQAAATLGQ
                     LAAGEVDIVIGTHKLLSRDVKFQRLGLAIIDEEHRFGVRQKEQLKALRAEVDVLTLTA
                     TPIPRTLALSLEGLREFSVIATAPQKRLAIKTFAAPFSEGLIREAALRELKRGGQVYF
                     LHNEVDTIQQMRERLARLLPEARIAVAHGQMRERELERVMRDFYAQRHSLLLCTTIIE
                     TGIDIPTANTIVIHRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPPEEALGAQAKKRL
                     EAIQMMEELGSGFYLAMHDLEIRGAGEVLGESQSGEIQEVGFNLYAQMLERAVRALKS
                     GRAVDMEQPLDIATEINLHAPALLPESYCSDVHERLTLYKRMANCESREQLDELREEL
                     VDRFGPLPEPARALIECHVLRLAARPLGVARVDATHETALLQFVKQPPIDGAKIIALV
                     RKRGDLRLSGPERLRLEAKMPGWPERAQAVRELLLQLAA"
     gene            15645..16343
                     /gene="ispD"
                     /locus_tag="AC16O9_06395"
     CDS             15645..16343
                     /gene="ispD"
                     /locus_tag="AC16O9_06395"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR6516281.1"
                     /translation="MTCVALIPAAGSGSRAAGTQPKQYAALAGRPMLWHAIAAVCRAP
                     VETVFVVLAPGDTAFKGCDWSAFAGKLEPLYCGGESRRDSVYNGLVAAMAAVDADDWV
                     LVHDAARPCLPAADLARLYEECRGDEIGGILALPVAETVKRAGKDEAGAQRVAGTEDR
                     AQLWLAQTPQMFRAALLAQALGNAKGAVTDEASAIEQMGLRPRLVAGSRENLKVTYAE
                     DLAIAEAILARRKP"
     gene            16340..16813
                     /gene="ispF"
                     /locus_tag="AC16O9_06400"
     CDS             16340..16813
                     /gene="ispF"
                     /locus_tag="AC16O9_06400"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR6516282.1"
                     /translation="MRIGQGFDVHALVPDRRLVLGGVHIPHHLGLEGHSDADVLLHAI
                     CDALLGAAGLGDIGGHYPDTDAQYAEVDSRRLLRDVAAKLAAKRLRIVNVDATVIAQA
                     PRLAPHFAAMKANIAADLGVAEGAVNLKATTTEGLGFTGRGEGIAALAVALIEER"
     gene            16815..17432
                     /locus_tag="AC16O9_06405"
     CDS             16815..17432
                     /locus_tag="AC16O9_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016207.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0043934 - sporulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HIR6516283.1"
                     /translation="MYSPPYNRVEDRRELVDFMRANNFALLVTATAGVPLASHLPVTV
                     ADGAGGIVIQSHMARPNAQWKEFFDDEVLVVFSGPHGYISPRWYEAAERVPTWNYAAV
                     HAYGTARVTTDKAAKHAAQRALVAALDPEWLPKFDALRAEYIESMLEGIVAFDVAVTR
                     LETRWKLSQNRGRREQELIAAALDKGADTQLEALAALTRKHFAER"
     gene            17434..17907
                     /gene="thpR"
                     /locus_tag="AC16O9_06410"
     CDS             17434..17907
                     /gene="thpR"
                     /locus_tag="AC16O9_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR6516284.1"
                     /translation="MRLFFACWPPAGTAQALAAWARQAQRACGGRVARAENIHLTLAF
                     LGDAEPALARARGAGVRLPASALCLGLAGHWAHNRIVWAGPQETPAPLAQLARALGES
                     RPFAAHVTLIRKARSPGPLPQLPALAWPVDHFTLVSSVLGRDGPSYEILERYALE"
     gene            complement(18142..>18974)
                     /locus_tag="AC16O9_06415"
     CDS             complement(18142..>18974)
                     /locus_tag="AC16O9_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6516285.1"
                     /translation="ISLVWGFNWVVLKIGVRHADPFEFLAWRFALSAACLFGAAAVLR
                     RPMRLAHAPQVLLIGLLQTTATFALATWALKSGAVGKTAVLNYTMPFWVALFAWPLLG
                     ERPSAAQGVALGVGLAGLLLLIHPAGAPGMPEVLALASGIAWGLGVVVTKRLQVRARI
                     DTLALIAWQVLFGGALLAALAVVFPGEPARWNTELVFAIAYNGILVSAVGWFLWFWAL
                     ARLDAGLASFGILAVPVLGALFSAALLGERPSALEWSGMATIVVALALAARAAIRRPS
                     "
BASE COUNT         2937 a         6438 c         6918 g         2681 t
ORIGIN      
        1 gcgcgagaag gcgcacgccg ccgagcgggc gcggcgcgag gccgcagagc aggcgcgccg
       61 cgaagccgag gagctgcggt tcaagatcga ggaggagcgc ctcgcgcgcg aggaagccga
      121 gcgccgcgcg cgcgcggaag ccgagcgcac caagcgcgag gcggcggaac gggcgcggcg
      181 cgaggaggag gagcgcaagg cgcgcgcaga agccgaggag ctgcgcaaga agtacgagga
      241 agaaaggaag gcgcgcgagg aagccgagcg ccgcgccgag gaagagcgcc agcgccgcga
      301 gcagcagcgg ctggaggaag agcgcaaggc gcgcgaggag ttcgagcgcc aggaagccga
      361 gcggcgcgcc gccgatgaag ccgagcgccg ggcgcgcgaa gccgaggagc gcaaggaggc
      421 cgaggaacac cagcgccaga tccgcctcgc gcgcgaggcc gaggacaaga agcgccgtga
      481 cgccgaggcg cgcaagaaaa aggaggcgga ggcgaagcgc tgggaagtcg aggaggcgcg
      541 gcgcagggcc gacgatgcgt accgccagcg cgccgagcag gaagacaagg agcgcgccga
      601 gcgcgaggcg gtggaggctg cggagcgcgc cgaggccgac aagagggccg ccgcggaagc
      661 cgacgccctc gccggctcgc tgctcgccga cctcgacagc ttctccagcc gccaggacga
      721 ggagcgcaag accaaggaag agcaggagcg gcaagagcgc gccgaggccg agcgcctggc
      781 cgcgcaagag cgcgcgcagc gcgagcgcga agaagccgag cgcaaggcca aaaagaccga
      841 ggagcgccgc cgccgcgagg aggaggagca gcgtgagcgc gaggcaaagg agcagcgcga
      901 gcgcgaggaa gaggcgcggc gcaaggaaga ggaagccgag cgcaagagaa aagagcgcga
      961 gctagccgcg cgcaacaagg aggtcaccga catcccgatc tccagcgccg acctcgaccg
     1021 cggcgacgtc gaggacgacg agcgcaagct cacgcccgag gcgcgcaagg cgctgcgcga
     1081 ccgcgagcgc gaggtggcgc gcgcgcgcag ccgcggcgac gtctcgggca agaaggcggc
     1141 cgccgcctac aagcgcaagc aacccaccca gtggggcaag ccagtcgcca tcttcctttt
     1201 cctcacgctg gtgatcagcg cgggcgtcgc caacttcatc ccgctcgata ccgcgactta
     1261 cgaaaaggcg gcgagcgacg cggtgggcgc gcccgtaaag atcggcgccg cgcgcctctg
     1321 ggtgctgact ggcgtgcagc tgcgcttcga gcgcgtcacg gtgggcgacg ccgtgaaagt
     1381 cgccgagatc cgcgcggtgc cggagttcgc ttcgctcctc ggcgacgaaa ggatattctc
     1441 gcgacttgaa ttcaacaggg cggtggtgca gcaaggggcg ctcggcaatg ccctgttcgg
     1501 caccatgaag caaggcgggc tgaaggtcgc gcgcgtcgtg gcgaaagacc tgcgcttcga
     1561 ggggccgatc gccctgccgc gaatggacat tgacgccgcg gtcggacccg acggccgcgt
     1621 gagctcggtc aggctctcgg gcgagcgcgt gaacggcctg ctcacgctgc gcgaggaccg
     1681 cgtcggcttc gaagtgacga tgggcagctt cacggtgccg ttcgtggaca agctgacgct
     1741 cgccgagttc ggcatgaagg gcagcgccga gagcggcggc ctgagcgcgg atttcgacgg
     1801 ccgcctctac gatggcacgg tggtgggcaa ggcgcgcgtc gagtggggcg cgcagtggag
     1861 cgtgcagggc gagatccagg gccgcaccat gaatgccgcc gtgttcgcgc cgcagatggt
     1921 gtccgagggg cgcttcgacg gcaagggccg cttcgcgatg agcggcgccg agccggcgca
     1981 gctcgacgac agcgcgcgcc tggacgggga ctttacggtg accaagggcg cgctcggcag
     2041 cttcgacctg tcgcgcgcgc tgcagtcggc gagcgcgcag accagcgggc gcacgccgtt
     2101 ctccgagctc gaaggccggg cgtcgctcgc cagcggcgcg ctcgcctttc gcgacctgaa
     2161 gctcaccgcg ggcctgctca acgcccgggg cgcgctcgac gtcgatgcca agggcgggct
     2221 gtcggggcgc atcgacgccg agctgcgcaa cctgcgcagc acgctctaca tcggcggcag
     2281 gctgagcgac ccgcagctgc ggcggtgatg ctcctgcagc ttcgcggcgc gcgcgcactt
     2341 tcggacttcc gcctcgccaa gctcctgccg ctgctgcagc gcgcggcgcc gggcgtgcgc
     2401 gccgtgcgcg ccgagttccg ccatttcgtc gagctggacg gcgcgccgtc ggcgcaggac
     2461 gaagcgctgc tcgcgcggct gctgcgctac ggcgcggcgc cgctggagcc cgggggcggc
     2521 gacacgctgt acctcgtggt gccgcgcatc ggcaccctgt cgccctgggc gtcgaaagcc
     2581 accgacatcg cgcgcaattg cggcctgacg caggtgcggc gcatcgagcg cggcaccgcc
     2641 tactacgtcg ccggcgccgc gcgctcggcg gccgtggcgg cgctgctgca cgatcgcatg
     2701 accgagaccg tgctcggcgc gttcgaggag gccgcgcagc tcttccggca cgtgccgccg
     2761 cgcccgctcg gccatgtggc actcgccgcg ctggacgagg ccaacgcgcg cctcggcctt
     2821 gcgctgtcgg gggacgagat ccaatacctg cgccaggcct acgcggcgct cgggcgcgac
     2881 cccaccgacg ccgagctcac catgttcgcg caggccaact ccgagcactg ccggcacaag
     2941 atcttcaacg ccgactgggt cgtcgacggc gagcgccagc cgcgctcgct cttcgccatg
     3001 atccggcaca cgcacgcggt gcagccgcgc ggcaccgtgc tggcctatgc cgacaacgcg
     3061 gcgatcatgg aaggacgcct cgcgcagcgc ttctacgccg gcgcggacgg ccgctacgcg
     3121 gcgcacgcgg agcccacgca cacggtgatg aaggtagaga cgcataacca tcccaccgcc
     3181 atctcgccct ttcccggcgc ggcgaccggc gcgggcggcg agattcgcga cgagggcgcg
     3241 accggccgcg gcgcgaagcc caaggcgggg ctggtcggct actcggtgtc gaacctgcgg
     3301 attcccgggt cagagcgtgc ctgggaggac gactatggca agcccggacg catcgtctcg
     3361 gcgctctcca tcatgctgga ggggccgatc ggcgcagctg ccttcaacaa cgagttcggc
     3421 cggcccaacc tcgcggggta tttccgcacc ttcgaggccg gggtgggcgg cgtgcgccgc
     3481 ggctaccaca agccgatcat gctcgcgggc ggcgtcggca atatcgccgc gcgcgattcc
     3541 gccaaagccc cgctgcccga gggctcgctg ctgatccagc tgggcgggcc cggcatgctg
     3601 atcggcatgg gcggcggcgc ggcctcgtcc atgggcgcgg gctcgaacgt cgaggacctc
     3661 gacttcgact ccgtgcagcg cggcaacgcc gagatccagc gccgcgcgca ggaagtgatc
     3721 gacgcgtgct gggcgctggg gcaaggcaat cccatccttt cgatccacga cgtgggtgcg
     3781 ggcggactgt ccaacgcgct cccggagctt gcgcacggcg ccgggcgcgg ggcgcggctc
     3841 gacctgcgcg cggcgccgtc cgaggactcg ggcatgtcgc cgcgcgagat ctggtgcaac
     3901 gaggcgcagg agcgctacgt gctcgccatc gcgccctcgt cgctcgagcg ctggcgcgcg
     3961 atctgcgcgc gcgagcgctg cccgttcgcg gtgctcggca ccgcgacggc cgacgggcgg
     4021 ctgcgggtgg aggatccgca gctcgcggac acgccggtcg acgtgccgct ggaggtgatc
     4081 ctcggcaagc cgccgaagat gctgcgcgac gtgaagcgcc tggcgcggcg gggggatgcg
     4141 ttcgcgctgg acggcgtggt gctgaaggac gccgcttatc gcgtgttgcg ccatccggcg
     4201 gtggcggaca agacgttcct catcgccatc ggcgaccgca cggtgggcgg gctgtgctcg
     4261 cgcgatccgt tcgtcggtcc ctggcaggtg ccggtggccg attgcgccgt gaccctgatc
     4321 gacttcgacg gcgtggcggg cgaggccttc gcggtgggcg agcgcacgcc gctcgcgtcg
     4381 gtgaacgcgc cggcctcggg ccgcatggcg gtgggcgagg cgctcaccaa cctcgccgcg
     4441 gcgcggattg aaagcatcga gcgcgtgaag ctctcggcca actggatggc ggcggcgggc
     4501 tatccgggcg aggacgcggc gctcttcgac acggtgcagg cggtggcgat ggagctttgc
     4561 ccggcgctcg gcgtgtcgat cccggtgggc aaggattcgc tctccatgcg cacggcgtgg
     4621 gaggggaagg aagtggtggc cccggtgtcg ctcgtcgtgt ccgcgttcgc gccggtggag
     4681 gatgcgcgcg gcacgctcac gccgcgcctg cgcaccgacg cgggcgagac cgagctggtg
     4741 ctcgtggatc tggggcgggg gaagaaccgg ctcggcggct cgatcctcgc gcaggtgcac
     4801 gccaagttcg gcaatgaggt gccggacctc gacgatccgg cgctgctgaa gggattcttc
     4861 ggcgccattc agaagctggc tgcagagcgc ctcgtcctcg cctaccacga ccgctcggac
     4921 ggcggtctgt tcgccacggt gtgcgagatg gcgttcgccg gacactgcgg cgccacgctg
     4981 aacctcgacg ccatcgcctt cgacgcgctc accgaggacg tcgacgggtt ccgcaagagc
     5041 ggcgaggagc agctctccgg gcgcgtgcgc gagcagatcc tgaagtgcct gttcagcgag
     5101 gagctgggcg cggtgctgca gatccgcgcc gcggaccgcg cgaaggtgat ggacaccctg
     5161 cgcgctgccg gtctggggcc agtgtcgcac ctcatcggca cgctgaacga tcgggacgaa
     5221 atccgcttca cgcgcaacgc caaacccgtg ttcgcggagc cgcgcgtcga gctgcagcgc
     5281 gcctggtcgg agaccaccta cctcctgcag aagctgcgcg acaatcccga gtgcgcgcag
     5341 gaagagtacg accgcatcct ggatgcgggc gatccgggac tgcacgtcgc gctcaccttc
     5401 gatccgaagg aggtgttcgt caacaccggc gccgcgccgc gcatcgcggt actgcgcgag
     5461 cagggcgtga acgggcaggt ggagatggcc gcggcgttca cgcgcgcggg cttcgaggcg
     5521 atcgacgtgc acatgtccga cctcatctcg ggacggctgt cgctcgactc attcaaggga
     5581 ttcgccgcct gcggcgggtt ctcctacggc gacgtcctcg gcggcgggca gggctgggcc
     5641 aagtccatcc tgttcaattc gcgggcgcgg aaagcgttcg aggcgttctt tgggcgcagc
     5701 gacacctttg cgctgggcat ctgcaatggc tgccagatga tgtcggcgct gaaggagctg
     5761 gtgccggggg ccgggcactg gccgtcgttc gagcgcaacc gctccgagca gttcgaggcg
     5821 cggctcgtga tggtagaaat cgtcgacagc ccgtcgatcg tgttcaaggg catggccggg
     5881 agccgcattc ccggtgtcac cgcgcacggc gagggaaggg cgcagtttgc ttccgaaacc
     5941 gatcgcaaga aggcaatcgt cgccgcgcgc tttgtcgaca accgcggcaa ggtgacggag
     6001 acctatccgc tgaaccccaa tggctcgccg gaagggatca cgggcctgac caccgccgac
     6061 gggcggttca ccatcctcat ggagcatccg gagcgggtgt tccggacggt gcagatgtcc
     6121 tgggcgccgg acgaggcggg cgaggattcg ccgtggatgc ggatgttcag gaatgcgcgg
     6181 gggtgggtgg ggtcggggtg ggattcttgt ttcgagatgg cagttgggcg ggtatatgcg
     6241 ggtggatata tgccggcata tggcggtatc tacaggcaga tggttcaatc tgtataccga
     6301 tagttcttcc tggctttcgt tgcggcctaa tcactagtta gctttcgcca agaggcatca
     6361 tgaaaaagcg catcttggct atcttggcct atatgctccc gagctttccg ctcatgttcc
     6421 tctggcatct gacgattctt gccgacgcgt tcaccgcgcc ggaatagtcg cgcatcgacg
     6481 acgcgctggt cggccattgg acgagtggcg aaggaaagcc gggacgggta cgctgacggg
     6541 ccgcacgatc cggatttcca agccatgact caacccgtct acgccctcaa tctcttcaac
     6601 gtcgccgaca aggacgagta cctcgcctac tcgcgccgct cgcacaagga ggtcatggcg
     6661 cacggcggca aggtcgtcgc gctcggcaag ttccgcgagg cggctgccgg ctcgatcgag
     6721 ccgcgcgcgg tcatgatcct cgtggagtgg gcctcgaagg cggccttcga gagctaccgc
     6781 gacgacccga agctcgccga cctgcatccg caccgcgtga acggcacccg cgactacatc
     6841 tggcacctgt tcgacaagct cgaggacctg aggccgatcc tcaggtagcc gcgcggcccg
     6901 ctcgccggca ccctggggcg agcccgcggc gggcaggccg agcagcagga acaggagcag
     6961 gctgccggag cgcggagcgc gctgcgccgg gaacacgcga ccggttctag ccggcgtgcg
     7021 gctatcatcg gggcagccat gacgctgatc acgcacaggg gcggctgcca ttgcgggcgc
     7081 gtgcgcttcg aggtcgacgc ccccgcgcac atcgaggtga ccgactgcaa ctgctcgatc
     7141 tgcgcgaaga ccgcgtacct gcatctcatc gtgccgcggg cccgcttcag gcttctcagc
     7201 ggcgaggatg cgctcaccac ctacgagttc aacacccgca cggccaagca cctgttctgc
     7261 tcgacctgcg gcatcaaggc gttctacgtg ccgcgctcgc accccgacgg tgtcagcgtc
     7321 aacgcccgct gcctcgacga gggcacggtg acggggatgt cgatccgccc caccgacggc
     7381 cggaactggg agcaggccta ccccgagggc cgcggcgctt tcttggagta gtcgaccggg
     7441 cgcggtgcgg gggtgtctaa ttcaccctgg cacacagcca aggaggccac cgccatgccg
     7501 acgttcatcg cgcttgcgaa cttcaccgac cagggggtca agaccgccaa ggacagcgtc
     7561 aaccgcgccg aggcggtcaa ggccatgggg gaaaagtacg gggtcaagct gaaggacatt
     7621 tactggacca tgggcgccta tgacgtggtg gcgatcttcg aggcgccgga cggcccgacc
     7681 atgtcggccg tgtccctggc agtcgccgcc gcgggcaaca tccgctcgca gacgctgcgc
     7741 gcgtacacga aggaggagat gaaggaggtc ctgaagaagc tgccgtgatg gccgagaccc
     7801 gccccgatcc ggacgtggcg gtcgaccgcg aggcgatcga ccgccacgtg cgcaagctgg
     7861 tcgcggagaa ggcccggcgc gacgccgaga agttcgagcg cctgcgccgg cgcccgctgt
     7921 cggtcgccga gacgctggcc ctcgtgctcg ccgccgccgc ggtggtgacg gtgggggcgc
     7981 tggcgctgat cgcgctgcgc taggcggccg cggttaatga gacaatgcgg gcatgagcga
     8041 tcctgaacac caagcgcgcc tcgcccggct gcgggaactg ctgcagattc ccgaccgcga
     8101 tcggaccgac gagcagtggg acgagctgaa cgagctcgaa atcgtgctgg ccagcagcaa
     8161 ccggcagggc gcgccgcgcc ccgagcagca gggccagggc gggcagaacc gcccgcgcca
     8221 cggcggcggg ccgcatcccg gcaacccggg gcgcaagttc cacaaccgcc cgcccaagcg
     8281 ccgcggacca ccgcccggcc gctaggcgct caacctagaa gttcgaacag ggaggaacgc
     8341 atggccatca cgcgccacga cccgggaccg ctgctgtcgc tggcggtcga gcacggcgac
     8401 accgtctacc tggcgggcat cgtcgccaag aacctgaaga acgacgtcaa gggccagacc
     8461 gaggaagtgc tggccgagat cgaccgcctg ctcgccaagt gcggctccaa caagtcgaag
     8521 atcctgcagg ccaccatctg ggtgcccgac atccgcctgc gcaccgcgat gaacgaggtg
     8581 tggacgaagt gggtggaccc caagaacctg ccggggcgcg cctgcatcga ggcgaagctg
     8641 gccgacccgg cggcgctggt cgagatcatg gtgaccgcgg cgaagtaatc cgcggccacc
     8701 cggcggggga ttaccccccg cgcttgtaga cgatcttctt ggtgccgccg tcgcaggtgc
     8761 cgaccacctt gccgtcctcg ggcgcgtcct tgtcgacgat ggtggtggtg aacaccttga
     8821 cgccgttcgc ggtgatcttc gcgtcgatct cgctcttcag ctcgttgcaa tccttgcgct
     8881 gggcgagggc gggcgatacg gcgagggcga gggccagcgc cagtgcggtc ttggtcatct
     8941 tctgtctccc gtttgagttc ggataagcgc ggctattgtg caccggccgc gcccgcgctg
     9001 aaagagcatt aaggcgtcgc ccggtcccga tgcccccccg tgacgaccac cgcctgcgcg
     9061 cgcgcgcacc tgcagcgtgc gcagctcgcc gcaggcgagg ctggcgaggg gcaagatcag
     9121 ggcgacggtg gcgatccagc ggctcatagc atctccagcg ggcgcgcctt ctgcggtggc
     9181 gggaaggcag cgtcgcatgc tttcagggcc gcgtcgtcga gtttcagcgc cagtgccttg
     9241 tggttctcgc gcacgtgcgc caccgacgcg gccttcggta tcgccaccac gccgtcctgg
     9301 cgcagcaccc acgcgagcgc cacctgcgcc ggcgtggcgt tgaggcgctg cgcgacttcc
     9361 ttcagcgcct tgtggccgag gatccggcct tgctccaccg gcgagtacgc catgacggaa
     9421 atcttgcgct cgcggcacca gggcagcagg tcgaactcga tcccgcgccg cgcgaggttg
     9481 tagagcagct ggttcgtgac gcaggccttg ccgcccggca cggcgacgag ctggttcatt
     9541 tccccgaggt cgaggttgct cacaccccat gagcggatct tgcctgcggc gagcagctcc
     9601 tcgaagccgc gtaccgtgtc ttcgagcggg tgggaccccg accagtgcag gaggtagaga
     9661 tccaggcagt cggtcttcag ccgcttcagg ctcgcctcgc aggcgcgcac cacccccttg
     9721 cgcgatgcgt tcgagggcag caccttgctg acgagaaaga tcctgtcccg gattcccgcg
     9781 atggcttgcg cgaccaggga ctcggagcgg ccgttgccgt acatctcggc ggtgtcgatc
     9841 agcgtcatgc cgagctcgat cccggcgcgc agggcggcga gctcgtcctc gcgcgccgac
     9901 gcgctgtcgc ccatgttcca ggtgccctgg ccgagccggg gcattgccat ggccgaactc
     9961 aggccgcgac cgaaggcagc ccggcgagcg ccatcgccag ttctttctcg tcgtatttct
    10021 ggtcggtgag cttgcccgag aagtaatccg tatacgcctg catgtcgaag tggccatggc
    10081 cgcagaggtt gaacaggatc acgcgcgatc tgccctcggt cttgcagcgc agcgcttcgt
    10141 cgattgccgc cttgaccgcg tggttcgcct ccggcgcggg aatgatgcct tcggcgcgcg
    10201 cgaactgcac gccggccgag aacacctcgg tctgcgcgta ggcgcgcgac tcgagcaggc
    10261 cgagttcctg gcagtgcgac acgaggggcg acatgccgtg gtagcgcagc ccgccggcgt
    10321 ggaacgaggg tggcgtgaag gtcgagccga gggtgtgcat cttcaccagc ggcgtcaggt
    10381 ggccggtatc accgaagtcg taggcgtact tgccgcgcgt gaggctcggg caggccgcgg
    10441 gctcgaccgc gatcacgcgc actttcttgc cgccgcgcag ctgcgcgccg aggaagggga
    10501 acaccaggcc ggcgaagttc gagccgccgc cggtgcagcc gatcaggatt tccgggtagt
    10561 cgcccgccat ctccatctgc gccatcgcct cctggccgac gacggtctgg tgcgtgagca
    10621 catggttgag caccgagccc agcgcgtact tggtgtcgtc gttggtggcg gcgacctcga
    10681 cggcctccga gatcgcgatg cccaggctgc ccgggtgatc cttgcgctgc gcgaggatgg
    10741 cgcgcccgga gttggtttcc ggcgaggggg aagcgatgca tttcgcgccg taggtttcca
    10801 tcatggcgcg gcggtacggt ttctgctcgt acgaaacgcg caccatgaac accttgatct
    10861 cgatgccgaa cagcgcgccg gcgaaggcga gcgacgagcc ccactggccg gcgccggtct
    10921 cggtggtgat cttcttgacg ccggcttcct tgttgtagaa ggcctgcgcc acggcggtgt
    10981 tcggcttgtg gctgccggcg ggcgagacgc cctcgtactt gtagtagatc ctggcggggg
    11041 tctgcagggc gtgctcgagg cgccgcgcgc ggtacagcgg tgtcgggcgc cactgcttgt
    11101 agatgtcgcg caccggttcc gggatctcga tctcgcgctc ggtcgagacc tcctggccga
    11161 tgagcgccat cggaaacagc ggcgccaggt cgtccggccc gatcggctgc ttggtgccgg
    11221 ggtgcagcac cggcggcagg ggcttgggca gatccgccgc aatgttgtac cagcgcttcg
    11281 gaatgcgcgt ttcgtcgagg atgtacttca ctgaatcagc catcgggtcc cctccttgtt
    11341 ggaaaatgat cctagcgcaa aaaagcggcc ggtgaggtgc ggcgcgccta gtcggcgaac
    11401 aggtgcaggg cgccgtccag gccggcatgg tcgagcgcgt gcgtggcacg cgcgcgcacc
    11461 gcgggcttgg cgcggtaggc gatcgaggtg cccgcgaccg acatcatggg gatgtcgttt
    11521 gcgccgtcgc cgatggcgag cgcttgctcg ggccgcaggc cgagccggcg catctgctcg
    11581 cgcagcttct cggccttggc gcggccgtcc acgatcggac cgagcacgcg cccggtgagc
    11641 cggccccgct cgatctcgag cgcattggcg agcgccgcgt cgatgccgag ccgccgctgc
    11701 agccgggagg tgaagaaatc gaagccgccc gagacgagca gggtgcgaat gccgcgctcg
    11761 cggcagcggc gcaccagcgc ctcggcgccc ggcgaaaggt gcatgcgctg cgcccagatc
    11821 ctctccatcg ccgcctcctc gagtccggcg agcaggccga cgcggcggcg cagcgactcc
    11881 gcgtagtcga tctcgccgct catcgcctgc gccgtaatct gggccacgcg atccttgatc
    11941 cccagcatct cgcccatttc gtcgatgctc tcgatggtga cgagcgtgga gtccatgtcc
    12001 atggccacca ggcgcaggtc ggccatccgg cgaccgggcg gcacccacgc gcagtcgagc
    12061 ttgtgctcgt ggcaccactc gatcaccgct gcgttcctcc cggccccaag caggcggaac
    12121 gcggcatcgg cgagctgctc gatctcgcgc gcgccgcaca ggcgcgcggc ctcggccgcg
    12181 tgcgcggcgc tcgcccccgg accctgcagg acgaggttca cgccgcgagc tgcagcagca
    12241 gctcgcgcac cgcctgcgcg cgctcgggcc agcccggcat cttcgcctcc agccgcaggc
    12301 gctcggggcc cgagaggcgc aggtcgccgc gcttgcgcac cagcgcgatg atcttcgcgc
    12361 cgtcgatcgg cggctgcttc acgaactgca ggagcgcagt ctcgtgcgtg gcgtcgacgc
    12421 gcgccacgcc gagcggccgc gccgccagcc gcagcacgtg gcactcgatg agcgcgcgcg
    12481 ccggctcggg cagcggcccg aagcggtcga cgagttcctc gcgcagctcg tccagctgtt
    12541 cccgcgactc gcagttcgcc atgcgcttgt agagcgtgag gcgctcgtgc acgtcgctgc
    12601 agtaggactc gggcaggagc gccggcgcgt gcaggttgat ctcggtggca atgtcgaggg
    12661 gctgctccat gtccaccgcg cgcccggact tgagcgcgcg caccgcgcgc tcgagcatct
    12721 gcgcgtagag gttgaagccc acctcctgga tctcgcccga ctgcgactcg cccagcacct
    12781 cgcccgcgcc gcggatctcg aggtcgtgca tcgccaggta aaagcccgag ccgagctcct
    12841 ccatcatctg gatcgcctcg aggcgcttct tcgcctgcgc gccgagcgct tcttccggcg
    12901 gcgtgagcag gtaggcgtag gcctggtggt gcgagcgccc gacgcgaccg cgcagctggt
    12961 gcagctgcgc caggccgaac ttgtcggcgc ggtggatgac gatggtgttg gcggtcggga
    13021 tgtcgatgcc ggtctcgatg atggtggtgc agaggagcag gctgtggcgc tgcgcgtaga
    13081 agtcgcgcat cacgcgctcg agctcgcgct cgcgcatctg cccatgcgcc acggcgatgc
    13141 gcgcctcggg cagcagccgc gccagccgtt cgcgcatctg ctggatcgtg tcgacctcgt
    13201 tgtgcaggaa gtacacctgg ccgccgcgct tgagctcgcg cagcgccgcc tcgcggatca
    13261 ggccttcgga gaacggcgcg gcgaaggtct tgatggcgag gcgcttctgc ggcgcggtgg
    13321 cgatcaccga gaactcgcgc aggccctcga gcgaaagcgc gagcgtgcgc gggatcggcg
    13381 tcgccgtgag cgtgagcacg tcgacttcgg cgcgcagcgc cttcagctgc tccttctggc
    13441 gcacgccgaa gcggtgctcc tcgtcgatga tggcgagccc caggcgctgg aacttcacat
    13501 cacgtgacag gagcttgtgc gtgccgatga cgatgtcgac ttcgcccgcg gcgagctgcc
    13561 cgagcgtggc cgcggcctgc ttcgcggtgc gaaagcgcga cagctcggcg attctcaccg
    13621 gccagtcggc gaagcggtcg gagaaggtct ggaaatgctg ctcggcgagc agtgtcgtcg
    13681 ggcacaggag cgccacctgc ttgccgtcgg cgacggcgac gaacgccgcg cgcagcgcca
    13741 cctcggtctt gccgaagccg acgtcgccgc agatgaggcg atccatcggc ttggccgcag
    13801 ccatgtccgc aaccacggcc tggatggcag cggcctggtc gggggtctcc tcgaagccga
    13861 agccggccgc gaacgcatcc aggtcgtgcg cctgcagctt gaacgcatgg ccttcgcgcg
    13921 ctgcgcgccg cgcgtagagc gcgaggagct cggcggcggt gtcgcgcacc tgcttggccg
    13981 cgcgcgcctt ggccttgtcc cactggccgc tgcccagctt gtgcagcggc gcggcttcgg
    14041 cctcggcgcc cgagtagcgc gagatgaccg ccagctgcga caccggcacg tagagcttgt
    14101 cgccgccgtc gtactgcagg tgcaggaact cggtcggccc ttcgcccagg tccaggccca
    14161 ccagcccctg gtagcggccg atgccgtgct gcgcgtgcac caccggatcg ccgatgcgca
    14221 gctccgagag gtcgcgcagc atgccctcga ccgagctttg cttttgccgg tcgcgcgcgc
    14281 gttgacgcac cgtgccggcg tagagctcgg cctcggtgac gacgcaccag ccctcggccg
    14341 gcagcgcgaa gcccgcgttg agcggcgcca cggtgagggc gaagcgctcc ggcgagcgcg
    14401 accaggccgc gaagtcggcg agcgcgacgg gcgcgagccc gtactccgcg aagtaggcga
    14461 gcatggtctc gcgccggccc ggcgactcgg ccgccaccag cacacgcagc gcgctggatt
    14521 cgaggaaccg cttgaagccc gcaagcgggt cctgtgcgcg ccggtccacc gcgagcggcg
    14581 gcagcggcgc gcttgccagc accgactcgg gctcgacgcg ccctcgggcg cgcgccgcga
    14641 cgtgaaactc ctcggccggc acgaagatcg tcgccggcgg caggagcggg cgctcgtgct
    14701 cgccgccgag cagcttgtag cgcgatgctg cgtcacgcca gaacgcgtcc accgcgccgg
    14761 gcacgtcgcc gtggcgcgcg agcgcgcagt gcgcgggcag gtagtcgaag agcgtcgcca
    14821 cctcgtcgaa aaacagcggc aggtagtact cgacgcccgc ggcgggcacg ccgttggaga
    14881 cgtcgcgata caggcccttc ttcgacggat cgccctcgaa cacttcgcgc cagcggctgc
    14941 ggaatttggc gcgcccggcc tcgtcgagcg ggaactcgcg cgccggcagc atgcgcactt
    15001 cctgcaccgg gtagagcgtg cgctgcgtgt cgacgtcgaa ggtgcgaatg gagtcgatcc
    15061 gctcgtcgtc gaggtcgagc cggtagggca gctggctgcc cgtggggaag aggtcgatca
    15121 gcccgccgcg gatgcagaat tcgccgggcg ccacgacctg cgtgacgtgc tggtagcccg
    15181 cggtcgcgag ctgcgccttg agcgcctcgg cgtcgagccg cgtgtcctgc ttcagaaaga
    15241 aggtgcgtgc agcgaggtac gacggcgggc acaggcgcac cagcgcggtc tgcgccggca
    15301 ccaccgcgac gtcgaactcg ccgcgctgga tgcggtacag cgtcgccagc cgctcggata
    15361 ccaggtcctg gtgcggcgag aacgcgtcgt agggcagggt ttcccagtcc ggcagcaggc
    15421 acacgcgcag ctgcggcgcg aaccaggcca gctcctcgac caggcgctgc gcatcgcgcg
    15481 cgctggaagt gatcaccgcg agcggcgcgc gcgaggtgcc cagccgcgcc agcgcgagcg
    15541 cgtccgccga cccggcgagt cccgtcgggt gcgcacttcc gggaagcggg tcgcgcagca
    15601 tgccgattgc aattatactt gcttgaattt atggagtttt tgccttgact tgcgtcgccc
    15661 tgattccggc cgcgggcagc ggcagccgcg cggccggcac gcagcccaag cagtacgccg
    15721 cgctcgcggg ccggccgatg ctgtggcacg cgatcgccgc cgtgtgccgc gcgccggtgg
    15781 agacggtgtt cgtggtgctc gcacccggcg atacggcgtt caagggctgc gactggagcg
    15841 cgttcgcggg caagctcgag ccgctgtatt gcggcggcga gtcgcggcgc gattcggtgt
    15901 acaacggact ggtggcggcg atggcggcgg tggacgcgga cgactgggtg ctggtgcacg
    15961 acgcggcgcg cccgtgcctg ccggcggccg acctcgcgcg gctctacgaa gagtgccgcg
    16021 gcgacgagat cggcggcatc ctcgcgctgc cagtggccga aaccgtgaag cgcgcgggca
    16081 aggacgaggc gggcgcgcag cgcgttgccg gcaccgagga ccgcgcgcag ctgtggctcg
    16141 cgcagacgcc gcagatgttc cgcgccgcgc tgctcgccca ggcgctgggc aacgcgaagg
    16201 gcgccgtcac cgacgaggcg agcgccatcg agcagatggg cctgcggccg cggctggtcg
    16261 ccggcagccg tgagaacctg aaggtcacct acgccgagga cctggcgatc gccgaggcga
    16321 tcctcgccag gcgcaagccg tgaggatcgg ccagggcttc gacgtgcacg cgctggtgcc
    16381 cgaccgccgg ctggtgctgg gcggcgtgca catcccgcac cacctcgggc tcgaagggca
    16441 ttcggacgcc gacgtgctgc tgcacgcgat ctgcgacgcg ctgctcggcg cggcggggct
    16501 gggcgacatc ggtgggcatt acccggacac cgacgcgcag tacgccgagg tcgacagccg
    16561 gcggctgctg cgcgacgtgg cggcgaagct cgcggccaag aggctgcgca tcgtcaacgt
    16621 cgacgccacg gtgatcgcgc aggcgccgcg cctcgcgccg catttcgccg ccatgaaagc
    16681 caacatcgcg gccgacctgg gcgtggccga gggcgcagtg aacctcaagg ccaccaccac
    16741 ggaaggactc ggcttcaccg gccgtggcga gggcatcgcc gccctggcag tggcgctgat
    16801 cgaggagcgc tgacatgtat tcgccgccgt acaaccgtgt cgaggaccgc cgcgagctcg
    16861 tcgacttcat gcgcgccaac aacttcgcgc tgctggtgac cgcgaccgcg ggcgtgccgc
    16921 tcgcttcgca cctgccggtt accgtggccg acggcgcggg cggcatcgtc atccagtcgc
    16981 acatggcgcg gcccaatgca cagtggaagg agttcttcga cgacgaggtg ctggtggtgt
    17041 tttccgggcc gcacggctac atctcgccgc gctggtacga agctgccgag cgcgtgccga
    17101 cctggaatta cgcggcggtg cacgcctacg gcacggcgcg cgtcaccacc gacaaggcgg
    17161 cgaagcacgc cgcacagcgc gcgctggtcg ccgcgctcga tcccgagtgg ctgccgaagt
    17221 tcgacgcgct gcgcgcggag tacatcgaga gcatgctgga gggcatcgtg gcgttcgatg
    17281 ttgcggtaac gcgcctcgag acgcgctgga agctctcgca gaaccgcggc cggcgcgagc
    17341 aggagctgat cgccgcggcg ctcgacaagg gcgccgacac gcagctcgag gcgctcgcgg
    17401 cgctgacgcg caagcacttc gccgagcgct gagatgcgcc tgttcttcgc ctgctggccg
    17461 cccgccggca cggcgcaggc actggccgcc tgggcgcggc aggcgcagcg tgcctgcggc
    17521 ggacgcgttg cgcgagcgga gaacatccac ctgacgctgg cgttcctggg cgatgccgag
    17581 ccggcgctgg cgcgcgcgcg aggggcgggc gttcggctgc cggcgagcgc gctttgcctc
    17641 gggctggcgg gccactgggc gcacaaccgc atcgtctggg ccgggccgca ggagacgccg
    17701 gcgccgctgg cgcagctcgc gcgggcgctg ggtgaatcgc ggccgttcgc cgcgcatgtg
    17761 accctgatcc gcaaggccag gtcgcccgga cccctgcctc agctaccggc gctggcgtgg
    17821 ccggtggatc acttcaccct cgtcagctcg gtcctgggtc gggacggtcc gagctacgag
    17881 atactcgagc gctacgccct cgagtgagcc gggtcggggt gggggctgcc gaggcgccgg
    17941 acgaggccgg cgaggccaac caggaatgcg cctagcgcgt cagcttgcgc cagcctggcc
    18001 ttggccagct gccgctccgc cgctgccatc gggatctcgt cgaggcgtgc gtacaaagta
    18061 cttaagttgt tcatttaaag ctccaaatgc agaagtatgc tgcattgcat catactttgc
    18121 ggtggagcgc cttgaccccg atcaagaagg ccggcggatc gccgcgcggg ctgcgagcgc
    18181 caaggcgacg acgatcgtgg ccatgccgct ccattcgagc gcgctggggc gctcgccgag
    18241 gagcgccgcg ctgaacagcg cccccagcac gggcaccgcc aggatgccga agctcgccag
    18301 cccggcatcg aggcgcgcga gcgcccagaa ccagagaaac cagccgaccg cgcttaccag
    18361 gatgccgttg taggcgatgg cgaagacgag ctcggtattc cagcgcgcgg gctcgccggg
    18421 aaacacaacc gccagcgcgg cgagcagcgc gccgccgaac agcacctgcc aggcgatgag
    18481 cgcaagcgtg tcgatgcgcg cgcgcacctg gaggcgcttg gtgaccacca cgcccaggcc
    18541 ccaggcgatg cccgaggcga gcgcgagcac ctcgggcatc cccggtgcgc cggcggggtg
    18601 gatcaggagc agcaatccgg ccagccccac gccgagtgcg acgccttgcg cggccgaggg
    18661 ccgttcgccc agcaggggcc acgcgaatag cgccacccag aacggcatcg tgtagttgag
    18721 caccgcggtc ttgcccaccg cgccgctctt cagagcccag gtggcaagcg cgaaggtggc
    18781 ggtggtctgc agcaagccga tgagcaggac ttgcggcgcg tgcgcaaggc gcatcgggcg
    18841 gcggagcacg gcggcggcgc cgaacaggca cgccgccgac agggcgaagc gccaggcgag
    18901 gaactcgaac gggtcggcgt gccgcacgcc gatcttcagc accacccagt tgaatcccca
    18961 caccaggctg atgg
//
LOCUS       DBNHKE010000104        11996 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_493643, whole genome shotgun sequence.
ACCESSION   DBNHKE010000104 DBNHKE010000000
VERSION     DBNHKE010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11996
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_493643"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..747
                     /locus_tag="AC16O9_06420"
     CDS             4..747
                     /locus_tag="AC16O9_06420"
                     /inference="COORDINATES: protein motif:HMM:NF036686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR6516286.1"
                     /translation="MADALGWRRKLAVLVPSTNTVVQPEFDAMRPDGVTNHISRIRIP
                     NMPLASDADFRLLVERIAAAQLEAVDSAMSCEPDHVVLGISVETFWGGLAASRKLQAE
                     LEQHTGLGVTMGAQACDAAFAALGVKRIAVLTPYFPVGDRNVQAFFEESGYEVVRIQG
                     LKCGSPVLIAHVAPDTLRAAAAALDGDDVDAVIQVGTNLAMARLAARMEAERAKPVLA
                     INACIYWHALRTNGIAERIAGWGSLLQHH"
     gene            complement(764..1579)
                     /gene="phnE"
                     /locus_tag="AC16O9_06425"
     CDS             complement(764..1579)
                     /gene="phnE"
                     /locus_tag="AC16O9_06425"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR6516287.1"
                     /translation="MPLERRAHEILWRRQAPAAQAARFAGWLALVALTVYCFEVMTRN
                     TIWAFFLDAPRQAADLLGRMVPPRWSYINELWWPLWETLNIATVGTLLAIGMALPVAF
                     LAARNTTPSVAFVRPVALFIIVSSRSINSLIWALLLVSIIGPGALAGIVAIALRSIGF
                     VAKLMYEAIEEIDANQVEAITATGASRGQVLVYGIWPQILPAFAGVCVFRWDINIRES
                     TVLGLVGAGGIGLNLQASLNVLAWPQVTLIFLVILASVVASEWVSARVRHAFI"
     gene            complement(1579..2379)
                     /gene="phnE"
                     /locus_tag="AC16O9_06430"
     CDS             complement(1579..2379)
                     /gene="phnE"
                     /locus_tag="AC16O9_06430"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR6516288.1"
                     /translation="MSALAGQRWRRPPLITDARLRWALIIGALAYLALALASLEVNWN
                     RIAEGIPRALRFVAAFFPPDFVSRWGDISEGLLESLTMTVTSTVVGIAISVPIGIGAA
                     RNVAPAWIYVACRGIIAVSRSFQEVIIAILFVALFGFGPLAGFLTLSFATIGFLSKLL
                     AEDIEDIDPGQAEAIRATGASWLQWINYGIQPQVMPRLIGLSLYRLDINFRESAVIGI
                     VGAGGIGATLNTAIDRYEFDSAAAVLLIIIGIVMFAEYTSSWIRGKVQ"
     gene            complement(2376..3197)
                     /gene="phnC"
                     /locus_tag="AC16O9_06435"
     CDS             complement(2376..3197)
                     /gene="phnC"
                     /locus_tag="AC16O9_06435"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR6516289.1"
                     /translation="MLRIEGLTKRYRTGDLALDEVDLQVPGGQVMALIGPSGAGKSTL
                     IRCVNRLVEPSAGRVRLGETEITGLARAELRRARRRMGMIFQEFALVERLTVMENVLS
                     GQLGHVGFWRSYFRRFPQSAVDMAYATLERVGLAEHVDKRADALSGGQRQRVGIARAL
                     LQQPEILLVDEPTASLDPKTSRQIMRLICELCAERGLAAVINIHDVQLAQLFVQRVVG
                     LRAGKVVFDGPPADLNADVLTRIYGEEDWTAAATRRRGAEGDDDALDEERMAGIV"
     gene            complement(3211..4173)
                     /gene="phnD"
                     /locus_tag="AC16O9_06440"
     CDS             complement(3211..4173)
                     /gene="phnD"
                     /locus_tag="AC16O9_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6516290.1"
                     /translation="MSIRTSIAALALLAWTGAALGQDCPRGALDARFCDTDGDLVADT
                     PSDAGKLVDPPVLIFAYTPVEDPAVYAKVWDGFLAHMQKLTGKRVQFFPVQSNAAQIE
                     AMRAGRLHIAGFNTGSNPLAVNCAGLVPFAMMAHADDSFGYEMEIITYPGSGVNKVED
                     IKGKTMAFTSQTSNSGFKAPSALLKAEFNMIAGTDFKPTFSGKHDNSILGVANKDYPA
                     ASIANSVLKRMISRGVVKAEQVKTIYKSQTFPTTGYGHVYNLKPELAGKVKEVFFSFP
                     WEGSALKAEFEKSGEAKFIPITYKKHWDVIRKIDAANNVSYACK"
     gene            4366..4821
                     /locus_tag="AC16O9_06445"
     CDS             4366..4821
                     /locus_tag="AC16O9_06445"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6516291.1"
                     /translation="MPGNPVADFSLPATGGGTFRLSEQRGKKLVLYFYPKDNTPGCTT
                     EGMQFRDLHMNFQDAGCDIYGVSRDSLKSHENFKAKLHFPFQLLSDAEETACKQFGVI
                     KLKNMYGKQVRGIERSTFVLDGEGRLAREWRGVKVPGHAQEVLDFVKTL"
     gene            4772..6307
                     /locus_tag="AC16O9_06450"
                     /pseudo
     CDS             4772..6307
                     /locus_tag="AC16O9_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            6343..7185
                     /locus_tag="AC16O9_06455"
     CDS             6343..7185
                     /locus_tag="AC16O9_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516292.1"
                     /translation="MHWHHHAAAALAALALALPAAAQKDKDYEPHVGQAGKDVIWVPT
                     PDEVVDRMLRMAQVTANDYVVDLGAGDGKIAIAAAKKFGARALGIEYNEEMARHAQKN
                     VERAGVAGRARIVQGDIFVSDFSQATVVTMYLLPALNLKLRPQILAMRPGTRVVSHSF
                     SMDDWEADETSSLDGRRAYFWVVPAAVAGTWSLELAGGGAAEKMEMTLEQRFQKIEGT
                     VALGTVLGGLREARLRGFLIAFSYVDDKGVRREFNGRVSGARMEGSFRADNGTEGRWT
                     AAKR"
     gene            complement(7182..7427)
                     /locus_tag="AC16O9_06460"
     CDS             complement(7182..7427)
                     /locus_tag="AC16O9_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR6516293.1"
                     /translation="MADPMIPQKSPFAVELAPGDYWWCACGQSKRQPFCDGTHKGGPF
                     TPVKFSIAEAQKVWLCGCKHTGKQPFCDGTHQKVVQA"
     gene            complement(7465..8874)
                     /gene="dnaB"
                     /locus_tag="AC16O9_06465"
     CDS             complement(7465..8874)
                     /gene="dnaB"
                     /locus_tag="AC16O9_06465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6516294.1"
                     /translation="MALPQPAARNDGGDPQLVALRVPPHSVEAEQSLLGGLLLDNQAF
                     DRIADLVSGSDFYRDDHRRIWRHIAKLVEAGKPADMVTVSESIEASEDKDKTGGPAYL
                     GALAQNTPSALNIRRYAELVRERAVQRRLAQVATEIAENALSPSGKDVGQILDEAESR
                     ILEIGEAGARHGVGLEEIKPVLARVLERIDYLYHRDNPSDVTGIPTGFNDLDTKTAGL
                     QPGDLIIVAGRPSMGKTAFALNIAEHVALNALLPVAVFSMEMSGTQLATRLLGSIARV
                     DQHKMRTGRLSDQEWHDLSEAMGRLHDAPIFIDETAALNALEVRTRARRLWRQFGGKL
                     GLVVVDYLQLMSTANPKAGENRATELSEISRSMKALAKELAVPVIALSQLSRAVESRN
                     DKRPMMSDLRESGAIEQDADVILFIYRDEVYHPDKPEAKGRAEVIIGKQRNGPIGKVE
                     LTFLGQHTRFANFADPGTF"
     gene            complement(8984..9433)
                     /gene="rplI"
                     /locus_tag="AC16O9_06470"
     CDS             complement(8984..9433)
                     /gene="rplI"
                     /locus_tag="AC16O9_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR6516295.1"
                     /translation="MQVILLEKVANLGDLGEVVKVKDGFARNFLIPHGKAKRATEANL
                     KAFEARRAELEKAQAEQLAKARERGAKLDGLTVQIVQKAGPDGHLFGSVTNFDIVEAL
                     EKQGHEVERSQVRMPSGPLKQVGEFPIQVQLHTDVLVTVNVAVLGES"
     gene            complement(9444..9737)
                     /gene="rpsR"
                     /locus_tag="AC16O9_06475"
     CDS             complement(9444..9737)
                     /gene="rpsR"
                     /locus_tag="AC16O9_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR6516296.1"
                     /translation="MPPPRRGKFKGKGKFGKDKKDKGQRALFRRRKFCRFTADKVEWI
                     DYKDIEVLKDFVNETGKIIPARITGTASGYQRQLSAAIKRARFLALLPYTDLH"
     gene            complement(9740..10039)
                     /gene="priB"
                     /locus_tag="AC16O9_06480"
     CDS             complement(9740..10039)
                     /gene="priB"
                     /locus_tag="AC16O9_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HIR6516297.1"
                     /translation="MNQVRIRGRLIELGALRHTPAGLPAIEFRLRHESEQTEAGAQRQ
                     VNAEIPAIAFDVQARLLAGCALERAIEVRGFLAAKSRRSKKLVLHATHIEFAEGE"
     gene            complement(10036..10416)
                     /gene="rpsF"
                     /locus_tag="AC16O9_06485"
     CDS             complement(10036..10416)
                     /gene="rpsF"
                     /locus_tag="AC16O9_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR6516298.1"
                     /translation="MRHYEICFIVHPDQSEQVPAMIERYKGVIDQRKGTVHRLEDWGR
                     RQLTYPIQKVHKAHYVLMNIECDQETVNELEHSFKFSDAVLRHLIIKMTKAVTAPSPM
                     MKEEKSRSVMERAPEAKPAEASAT"
     gene            complement(10549..11343)
                     /locus_tag="AC16O9_06490"
     CDS             complement(10549..11343)
                     /locus_tag="AC16O9_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6516299.1"
                     /translation="MPTLEEFQFEHDGIKVCGLVQGSGPPVLMVHGMPSSRNVWALVK
                     DRISTRFRVVAMDLPGFGRSTKVALPSLDAFSDWLARVLDHVGEQSAHVVGHSFGGML
                     SLAFLTKFPKRVRSLVLCDSVGLGPYGGSEGQRRFAAARTREDVRAFLQRLFHNPDAI
                     SDALVEGQFAYVTQPGVPEVLEQLTARRQDWEAQVEAHADMIDVPLMVIWGRNDQLVP
                     VAHADRLRHVPGARFVIIENCGHAPMVERPQEFGEHMLEFLDSVAG"
     gene            complement(11515..>11996)
                     /locus_tag="AC16O9_06495"
     CDS             complement(11515..>11996)
                     /locus_tag="AC16O9_06495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516300.1"
                     /translation="SYDVAISSDCFEHNPCWLETFRNMLRLTREGGLVLFTCASTGYR
                     EHGTRRSAPEASPLTIAAGWDYYLNLTEADFTRRLDLRRWCSDYAFVADYESYRLCFV
                     GRRGEKGASPLPPLLVPNLNERFNVWGSARAMKRRVKVALFGNFLSSPLSYYFLGKR"
BASE COUNT         2001 a         4058 c         3985 g         1952 t
ORIGIN      
        1 tagatggccg acgcgctcgg ctggcgccgc aagctcgcgg tgctcgtgcc ctccaccaac
       61 accgtggtgc agccggagtt cgacgccatg cgcccggacg gcgtcaccaa ccacatcagc
      121 cgcatccgta ttcccaacat gcccctcgcc agcgacgccg acttccggct gctggtggag
      181 cgcatcgccg cggcgcagct cgaggcggtg gacagcgcca tgagctgcga gcccgaccat
      241 gtggtgctcg gcatctcggt ggagacgttc tggggcgggc tggcggcgag ccgcaagctg
      301 caggccgagc tcgagcagca tacgggcctc ggcgtgacga tgggcgcgca ggcctgcgac
      361 gccgccttcg ccgcgctcgg cgtgaagcgc attgcggtgc tcacccccta ctttcccgtc
      421 ggcgaccgga acgtgcaggc gttcttcgag gaatcgggct acgaggtggt gcgcatccag
      481 ggcctgaagt gcggctcgcc cgtgctcatc gcgcacgttg ccccggatac gctgcgcgcg
      541 gcggccgcgg cgctcgacgg cgacgacgtc gacgcggtga tccaggtcgg caccaacctc
      601 gccatggcgc gccttgcggc gcggatggaa gccgagcgcg ccaagcccgt gctcgccatc
      661 aacgcctgca tctactggca cgccctgcgc acgaacggca tcgccgagcg catcgcgggc
      721 tggggcagcc tgctgcagca ccactgagga gagcgcgcgt ggcctagatg aaggcgtggc
      781 gcacgcgcgc cgacacccac tcgctcgcca ccaccgaggc gaggatgacg aggaagatga
      841 gcgtcacctg cggccaggcc agcacgttga gcgaggcctg gagattgagg ccgatgccgc
      901 ccgcgcccac caggccgagt accgtcgact cgcggatgtt gatgtcccag cggaacacgc
      961 acacgccggc gaaggccggc aggatctgcg gccagatgcc gtagaccagg acctggccgc
     1021 gtgaggcccc ggtcgcggtg atcgcctcga cctggttggc gtcgatctcc tcgatcgcct
     1081 cgtacatcag cttggcgacg aagccgatcg aacgcagcgc gatggcgacg atgccggcga
     1141 gcgcgccggg cccgatgatc gacacgagca gcagcgccca gatgagcgag ttgatcgagc
     1201 gcgacgagac gatgatgaaa agggccaccg ggcgcacgaa cgccacgctc ggcgtggtgt
     1261 tgcgcgcggc gagaaacgcc accggcagcg ccatgccgat ggcgagcagc gtgccgacgg
     1321 tggcgatgtt gagcgtctcc cacagcggcc accacagctc gttgatgtag ctccagcgcg
     1381 gcggcaccat gcgcccgagg agatccgcgg cctggcgcgg cgcgtcgagg aagaacgccc
     1441 agatcgtgtt gcgcgtcatc acctcgaagc agtacaccgt gagcgcgacg agcgcgagcc
     1501 agcccgcaaa gcgcgccgcc tgcgccgccg gcgcctggcg ccgccagagg atctcgtggg
     1561 cccgtcgctc gagcggcatc actgcacctt gccgcggatc cagctcgagg tgtactcggc
     1621 gaacatcacg atgccgatga tgatcagcag taccgccgcg gcagagtcga actcgtagcg
     1681 gtcgatggcc gtgttcagcg tcgcgccgat gccgcccgcg ccgacgatgc cgatcaccgc
     1741 cgactcgcgg aagttgatgt cgaggcggta cagcgacagg ccgatgaggc gcggcatcac
     1801 ctgcggctgg atgccgtagt tgatccactg cagccacgag gcgccggtgg cgcggatcgc
     1861 ctcggcctgc ccggggtcga tgtcctcgat gtcctcggcg agcagcttcg acagaaagcc
     1921 gatggtggcg aaggacagcg tgaggaaccc cgccagcggc ccgaagccga acagcgccac
     1981 gaacaggatg gcgatgatca cttcctggaa gctgcgcgaa acggcgatga tgccgcggca
     2041 cgcgacgtag atccacgccg gagcgacgtt tctcgccgcg ccgatgccga tcggcaccga
     2101 gatggcgatg ccgacgaccg tggacgtgac cgtcatggtg aggctctcga gcagcccctc
     2161 ggagatgtcg ccccagcggc tgacgaaatc cggcgggaag aacgccgcaa cgaagcgcag
     2221 cgcgcgcgga atgccctcgg cgatgcggtt ccagttcact tccagcgagg cgagcgccag
     2281 cgcgagatac gccagggcgc cgatgatcag cgcccagcgc aggcgcgcat cggtgatgag
     2341 cggcggacgc cgccagcgct gcccggcgag cgcgctcaaa cgatgcccgc catgcgctcc
     2401 tcgtcgagcg cgtcgtcgtc gccctcggcg ccgcggcgcc gcgtcgcggc ggcggtccag
     2461 tcttcctcgc cgtagatgcg cgtcagcacg tcggcgttga gatccgccgg cgggccgtcg
     2521 aacaccacct ttccggcgcg caggcccacc acgcgctgca cgaacagctg cgccagctgc
     2581 acgtcgtgga tgttgattac cgcggccagg ccgcgctcgg cgcacagctc gcagatgagg
     2641 cgcatgatct ggcgcgaggt cttcggatcg aggctcgccg taggctcgtc caccagcagg
     2701 atctcgggct gctgcaggag cgcccgcgcg atgccgacgc gctggcgctg gccgcccgag
     2761 agcgcgtcgg cgcgcttgtc gacgtgctcg gccagcccga cgcgctcgag cgtggcgtac
     2821 gccatgtcca ccgcgctctg cggaaagcgc cggaagtagc tgcgccagaa gccgacgtgg
     2881 ccgagctgcc cggagagcac gttctccatc accgtgagcc gctcgaccag ggcgaactcc
     2941 tggaagatca tgcccatgcg ccggcgcgcg cggcgcagct cggcgcgcgc gaggccggtg
     3001 atctcggtct ctcccaggcg cacgcgcccc gcgctgggct cgaccaggcg gttcacgcag
     3061 cggatcagcg tcgacttgcc cgccccggag gggccgatca gcgccatcac ctggccgccc
     3121 gggacttgca ggtcgacctc gtcgagcgcc aggtcgccgg tgcggtagcg cttggtgagc
     3181 ccctcgatcc tgagcatcgc cgctacacgc tcacttgcac gcgtagctga cgttgttcgc
     3241 cgcgtcgatc ttgcggatca catcccagtg cttcttgtag gtgatcggga tgaacttcgc
     3301 ctcgcccgat ttttcgaact cggccttcag cgccgagcct tcccacggga aggagaagaa
     3361 cacctctttc accttgccgg caagctcggg cttgaggttg tagacgtgcc cgtagccggt
     3421 ggtgggaaag gtctgcgact tgtagatcgt cttcacctgc tcggccttga ccacgccgcg
     3481 cgagatcatg cgcttcagca ccgagttggc gatcgaggcg gccggatagt ccttgttggc
     3541 cacgccgagg atagagttgt cgtgcttgcc ggagaacgtc ggcttgaaat cggtgccggc
     3601 gatcatgttg aactcggctt tgagcagcgc cgagggcgcc ttgaagcccg agttggaggt
     3661 ctgcgaggtg aaggccatcg tcttgccctt gatgtcctcg accttgttca cgccgctgcc
     3721 cgggtaggta atgatctcca tctcgtagcc gaagctgtcg tcggcgtgcg ccatcatggc
     3781 gaagggcacc aggccggcgc agttcaccgc cagcgggttg gagccggtgt tgaagccggc
     3841 gatatgcagc cgcccggcgc gcatggcctc gatctgcgcc gcgttcgact gcaccgggaa
     3901 gaactgcacg cgcttgccgg tgagcttttg catgtgggcg aggaatccgt cccagacctt
     3961 ggcgtatacc gcggggtcct cgaccggggt gtaggcgaag atcagcaccg gcgggtcgac
     4021 cagcttgccc gcgtcgctcg gcgtatcggc gaccaggtca ccgtcggtgt cgcagaagcg
     4081 cgcatcgagc gcgccgcgcg gacagtcctg gccaagcgcc gcgccagtcc atgccagcag
     4141 cgcgagcgca gcgattgacg ttcgaatgct catggcttgc ctcctcgatg aaagtccctg
     4201 gagtgtacta aggccgccgc cctggcgaga aaaaggcctt ggaatcagcc ccctggcccg
     4261 ggcgcggcgg cggtgcgagc cgcctgcttg cggccgctgc aaaaaacgtg ttcaatgaaa
     4321 acgttgcgat tcacacctga gaccgcgggt taaatcagcg cctggatgcc gggaaatccc
     4381 gtcgcggact tctccctccc agccacgggc gggggcacgt tccgcctctc cgagcagcgc
     4441 ggcaagaagc tggtgctgta tttctacccg aaggacaaca cgcccggctg caccaccgag
     4501 ggcatgcaat tccgcgacct gcacatgaat ttccaggatg caggctgcga catctacggc
     4561 gtctcgcgcg actcgctcaa gtcgcacgag aacttcaagg ccaagctcca cttcccgttc
     4621 cagctgctgt cggacgccga ggaaacagcc tgcaagcagt tcggcgtcat caagctgaag
     4681 aacatgtacg gtaaacaggt ccgcggcatc gagcgcagca ccttcgtcct cgacggcgaa
     4741 gggcgcctgg cgcgcgaatg gcgcggtgtg aaggtgcccg gccacgccca ggaggtactc
     4801 gactttgtta agaccctctg attccccgct cgacgcccag cccgaacagg accgcaccga
     4861 cgcagccgca gacacccccc ccgccatggc gcacaagccc cgcccgccgc agaagaaatc
     4921 ccgcctcacc aagctcttcg ttcttgacac caacgtgctg atgcacgacc cggcgagcct
     4981 gttccgattc gaggagcacg acgtctacct gccgatcggc acgctggagg agctcgacga
     5041 ccacaagcgc gggctctcgg acgtggcgcg gaacgcccgc caggcaagcc gcttcctcga
     5101 cgagatcgtc tcggactcgg tggccgacat caaggcgggc ctgccgatcc gcacgcgcga
     5161 ccgccccggc gccggcgggc ggctgtacct gcagaccgaa gccatcaacg gcggcctgcc
     5221 gtcgaccttc gctgccggca agaccgacaa ccacatcctg caggtcgtgc gccacctgca
     5281 gaaggcgatg ccgcagcgcc cggtgatcct ggtctccaag gacatcaaca tgcgcgtcaa
     5341 ggcgcgcgcc ctcggcctcg cggcggagga ctactacaac gacaaggtgc tcgaggacac
     5401 cgacgtgctg tatgcgggcg tgcaggccct gccggccaac ttctgggaca cgcacgggcg
     5461 cgacatggag tcctggaaga aggagggcca cacctactac cgcgtgcgcg gcccgctggt
     5521 gcccaagctc cttctgaacg agttcgtgta cgacgactcg ggcgagcgcc cgctgtacgc
     5581 gatggtgaag gagaaatcgg ggcgcagcgc ggtgctggaa accgttcgcg actacacgca
     5641 cgccaagaat tcggtctggg gcatcaccgc gcgcaaccgc gagcagaact tcgccctcaa
     5701 cctgctgatg aacccggcga tcgatttcgt gacgctgctt ggccaggcgg gcacgggcaa
     5761 gacgctgctc gccctcgcct cgggcctcac gctggtgctc gaccagaagc gctacaccga
     5821 gatcatcatg acgcgcgtca cggtgccgct cggcgaggac atcggcttcc tgccgggcac
     5881 ggaagaagag aagatgcaac cctggatggg cgcgctcgag gacaacctcg atgtgctcaa
     5941 cgccacggac gaatccggcg gcgagtgggg ccgcgccgcc acccgcgacc tggtgcgctc
     6001 ccggatcaag atcaagtcgc tcaacttcat gcgcgggcgc accttcgtca acaagtggct
     6061 gatcatcgac gaggcgcaga acctcacgcc caagcagatg aagacgctgg tgacgcgcgc
     6121 cggcccgggc accaaggtgg tgtgcctggg caatattgca cagatcgaca cgccctacct
     6181 caccgagggc tcctccggcc tcacctacgt ggtggaccgg atgaaggact gggcgcacgc
     6241 cgggcacgtg acgctggcgc gcggcgagcg ctcgcgcctc gcggaccacg cggccggcac
     6301 gctgtaaact ccggcttccg tctagagcag aaaggaatcc tcgtgcactg gcatcaccac
     6361 gccgcggcgg cgcttgccgc gctcgcgctc gccctgcccg ccgccgcgca gaaagacaag
     6421 gactatgagc cgcacgtcgg tcaggccggc aaggacgtca tctgggtacc gacccccgac
     6481 gaggtggttg accgcatgct gcgcatggcg caggtcacgg cgaacgacta cgtcgtcgac
     6541 ctcggcgccg gcgacggcaa gatcgccatc gccgcggcga agaagttcgg cgcccgcgcg
     6601 ctgggcatcg agtacaacga ggagatggcg cgccacgcgc agaagaacgt cgagcgcgcc
     6661 ggcgtcgccg gccgcgcgcg catcgtgcag ggcgatatct tcgtgtcaga tttctcgcag
     6721 gcgacggtgg tcaccatgta cctcctgccg gcgctcaacc tcaagctgcg gccgcagatc
     6781 ctcgccatgc gccccggcac gcgcgtggtg tcgcattcct tcagcatgga cgactgggag
     6841 gccgacgaaa cctcgagcct cgacgggcga cgcgcctact tctgggtggt gccggctgcg
     6901 gtcgcgggca cgtggtcgct ggagcttgcc ggcggcggcg ccgcggaaaa gatggaaatg
     6961 acgctcgagc agcgcttcca gaagatcgag ggcacggtgg cgctcggcac ggtgctcggt
     7021 gggctgcgcg aagcgcggct gcgcggcttc ctgatcgcgt tttcctacgt cgacgacaag
     7081 ggcgtgcgcc gagaattcaa tgggcgggtg agcggcgcgc gcatggaagg atcgttccgc
     7141 gccgacaacg gcaccgaggg gcgctggacc gcggcgaagc gctaggcctg gaccaccttt
     7201 tggtgggtac catcgcagaa cggctgcttg ccggtgtgct tgcatccgca caaccagact
     7261 ttctgcgcct cggcaatcga gaacttcacc ggcgtgaacg gcccgccctt gtgcgtgccg
     7321 tcgcagaacg gctggcgctt ggactgcccg caggcgcacc accagtagtc gcccggcgcc
     7381 agctccacgg cgaacggcga cttctgcggg atcatcgggt cggccatcac gctctcctgc
     7441 ggctgaagcg gtccggagat tgtctcagaa cgtgcccggg tcggcgaaat tggcgaaacg
     7501 cgtgtgctgg ccgaggaagg tgagctcgac cttgccgatg gggccgttgc gctgcttgcc
     7561 gatgatcacc tcggcgcggc ccttggcctc gggcttgtct gggtggtaca cctcgtcgcg
     7621 gtagataaag aggatcacgt cggcgtcctg ctcaatggcg cccgactcgc gcaggtccga
     7681 catcatcggc cgcttgtcgt tcctgctctc cacggcgcgc gacagctgcg acagcgcgat
     7741 caccggcacc gcgagctcct tggcgagcgc tttcatcgag cgcgagatct ccgagagctc
     7801 ggtggcgcgg ttctcgccgg ccttcgggtt cgccgtcgac atcagctgca ggtagtccac
     7861 caccaccagg cccagcttgc cgccgaactg gcgccacagc cgccgcgcgc gcgtcctcac
     7921 ttccagcgca ttcagcgcgg cagtctcgtc gatgaagatc ggcgcgtcgt gcagccgccc
     7981 catggcttcc gacaggtcgt gccactcctg gtccgagagc cgcccggtgc gcatcttgtg
     8041 ctggtcgacg cgcgcgatcg agccgagcag ccgcgttgcc agctgcgtac cgctcatctc
     8101 catgctgaac accgccaccg gcagcagcgc attcagcgcc acgtgctctg cgatgttcag
     8161 cgcgaacgcg gtcttgccca tcgatggccg cccggccacg atgatcaggt cgcccggctg
     8221 cagcccggcg gtcttggtgt cgaggtcgtt gaagccggtg gggatgcccg tgacgtcgga
     8281 ggggttgtcg cggtggtaca ggtagtcgat gcgctcgagc acgcgcgcca ggacgggctt
     8341 gatctcctcg aggccgacgc cgtggcgcgc gcccgcctcg ccgatctcga ggatgcgcga
     8401 ttcggcctcg tcgaggatct ggccgacgtc cttgcccgag gggctgagcg cgttctcggc
     8461 gatctcggtg gccacctgcg ccaggcgccg ttgcaccgcg cgctcgcgca ccagctcggc
     8521 gtagcgccgg atattgagcg cgctcggcgt gttctgcgcc agcgcgccga ggtaggccgg
     8581 cccgccggtc ttgtccttgt cctcgctcgc ctcgatcgac tccgacacgg tgaccatgtc
     8641 ggcgggcttg ccggcctcga ccagcttggc gatgtggcgc cagatgcggc ggtggtcgtc
     8701 gcggtagaaa tcgctccccg agaccaggtc ggcgatgcgg tcgaaagcct ggttgtcgag
     8761 gagcaggccc ccgagcagcg actgctcggc ctctacggag tgcggtggaa ccctgagcgc
     8821 gacgagttgg ggatcgccgc cgtcgttgcg ggccgcgggt tgcggaagcg ccatccccgc
     8881 aatttacctc aaagcgcgcc gccgccgcgg caataaaaag gggccgcgcg cggcccctgt
     8941 gcattacctg tgattaagct gttcgcagcc tgtgcgcagc ctgtcagctt tcgccgagca
     9001 ccgccacgtt caccgttacg agaacatcgg tgtgcagctg cacctggatc ggaaactcgc
     9061 cgacctgctt gagcggcccc gagggcatgc gcacctggga gcgctccact tcgtggccct
     9121 gcttctccag cgcctcgacg atgtcgaagt tggtcaccga gccgaacagg tgcccgtcgg
     9181 gtcccgcctt ctgcacgatc tgcaccgtca gcccgtcgag cttcgccccg cgctcgcgtg
     9241 ccttggcgag ctgctcggcc tgcgcctttt ccagctcggc gcgccgcgcc tcgaacgcct
     9301 tcaggttggc ctcggtcgcg cgcttggcct tgccgtgcgg aatgaggaaa ttgcgcgcga
     9361 agccgtcctt caccttcacc acctcgccga ggtcgccgag gttggccact ttctccagaa
     9421 gaatcacttg catggcttgg ctctcagtgc aggtcggtgt agggcagcag ggcgaggaag
     9481 cgcgcgcgct tgatcgccgc cgacagctgg cgctggtagc cggacgcggt tccggtaatg
     9541 cgcgccggga tgatcttgcc ggtctcgttg acgaagtcct tgaggacctc gatgtccttg
     9601 tagtcgatcc actcgacctt gtcggcggtg aagcggcaga acttgcggcg gcggaacagg
     9661 gcccgctgcc ccttgtcctt cttgtccttg ccgaacttgc ctttgccttt gaacttgcct
     9721 ctgcgcggcg ggggcatctc tactctcctt ctgcgaattc aatgtgggtg gcgtgcagca
     9781 ccagcttttt cgagcgacgg cttttcgcgg cgagaaaccc ccgcacctcg atggcgcgct
     9841 ccagcgcgca gccggcgagc aggcgtgcct gcacgtcgaa ggcgatggcg gggatctcgg
     9901 cgttcacttg tcgctgggct ccagcttcgg tttgttcgga ttcgtggcgc aggcggaact
     9961 cgatcgccgg gaggcccgcg ggcgtgtgcc gcagcgctcc gagctcgatc agccggccgc
    10021 ggatcctgac ctgattcagg tcgcgctcgc ctcggcgggc ttcgcctcgg gcgcgcgctc
    10081 catcaccgag cgggacttct cctccttcat catcggcgaa ggggcggtca cggccttggt
    10141 catcttgatg atgaggtggc gcaacaccgc gtcgctgaac ttgaaggagt gctcgagctc
    10201 gttcaccgtc tcctggtcgc actcgatgtt catgagcacg tagtgcgcct tgtgcacttt
    10261 ctggatcggg taggtgagct ggcgccggcc ccagtcttcg agccggtgca cggtgccttt
    10321 gcgctggtcg atgacgccct tgtagcgctc gatcatcgcc ggcacctgct cgctctggtc
    10381 cggatggacg atgaaacaga tttcgtaatg ccgcatgctg tgattctcct tatgggttga
    10441 gccatccact catgcgcagg tggtatggca aggaaggggg cggattctac ttgaagatct
    10501 gccacttgca aagactttcg gtgctcgctg cgcgcagcct ccagcgcctt agcccgcgac
    10561 gctgtcgaga aactcgagca tgtgctcgcc gaattcctgc ggtcgctcca ccatcggcgc
    10621 atggccgcag ttctcgataa tgacgaatcg ggcgcccggc acatggcgca gtcgatcggc
    10681 gtgggccacg ggcaccagct gatcgttcct tccccatatc accattaatg gcacgtcgat
    10741 catgtccgca tgggcttcca cctgcgcttc ccagtcttgc cgccgagcgg tgagctgctc
    10801 aagcacttcc ggcacgccgg gctgggtgac atatgcgaac tgcccctcga ccagtgcgtc
    10861 cgagatagcg tcggggttgt ggaacaggcg ttgcaggaag gcgcgaacgt cctcgcgcgt
    10921 gcgtgccgcc gcaaatctac gctgcccctc acttcctcca tagggcccca ggcccaccga
    10981 gtcgcaaagc acaagcgagc gaacgcgctt cggaaacttt gtcagaaacg ctagcgagag
    11041 cattccgccg aacgagtgcc cgaccacgtg tgccgactgc tcgccaacgt ggtcgaggac
    11101 cctggccagc cagtccgaga acgcgtcaag cgatggcaag gcgaccttgg tcgaccgtcc
    11161 gaagcccggt aggtccatgg caaccacccg gaaccgggtc gaaatgcggt ccttgaccag
    11221 agcccacaca ttgcgggaac tcggcatccc atgcaccatt aggacgggcg gtccgctgcc
    11281 ctggaccagc ccgcagacct tgattccatc gtgctcgaac tggaattcct cgagggtcgg
    11341 catcgcctat gctccagccc ccgaaaggta cgcaccctac tcctgtgcga caagggattc
    11401 caccccgccg gcgggtggcg accgagtgac ggtcaaacga tgcagcgggc gtgcccacgg
    11461 ggattcgaac cccgactacc gtggaccctg aagtagtctc gacgataccg ccagctaccg
    11521 cttgcccagg aagtagtaag agaggggcga gctcaggaag ttgccaaaga gcgcgacctt
    11581 caccctgcgc ttcatcgcgc gcgccgatcc ccaaacattg aagcgctcgt tgagattggg
    11641 cacgagcagc ggcggcagcg gtgatgcgcc cttctctccg cgtcgaccga cgaagcacag
    11701 cctgtaggac tcgtagtcgg cgacgaatgc atagtccgag caccagcgcc gtaggtcgag
    11761 ccggcgcgtg aagtcggcct cggtcaagtt gaggtagtag tcccagcccg cggcgatcgt
    11821 gagcggcgag gcctccggcg cgctgcgccg cgtgccgtgt tcacggtagc ccgtcgaggc
    11881 gcaggtgaac agcaccaggc cgccctcccg cgtcaggcgc agcatgttgc gaaaggtctc
    11941 gagccagcat gggttgtgct cgaagcagtc ggacgaaatc gcaacgtcgt aggagc
//
LOCUS       DBNHKE010000105        15100 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_810893, whole genome shotgun sequence.
ACCESSION   DBNHKE010000105 DBNHKE010000000
VERSION     DBNHKE010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_810893"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="AC16O9_06500"
     CDS             <1..961
                     /locus_tag="AC16O9_06500"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516301.1"
                     /translation="VKQRESLLKQAQVDLERTIIRAPVDGTVILRNVDAGQTVAASLQ
                     APVLFTIARDLRDMQVEAAIDEADVGRLRPGLPATFTVDAFPRRGFSGEIVQIRKSPV
                     NVQNVVSYTVVISARNPDLALLPGMTANVRIVVEQRDNALKVPNAALRFRPAGAAEPA
                     PAAAAPQPGGGQALQQFRQRLVEAVQPDASQRAQLEEIFNDLRRKAAPLRELADEAER
                     RRQSERLRADMRTRVAEILKPEQRAAYEQLLRDYAGRAAVATGRVWVPDGRGTRAIDV
                     RLGLSDGLNTEIVAGELKEGAEVIVGLAATEQRGTGLPRLRLF"
     gene            961..1701
                     /locus_tag="AC16O9_06505"
     CDS             961..1701
                     /locus_tag="AC16O9_06505"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516302.1"
                     /translation="MGEPLIRTRDVAKEYRVGDNLIRALAGVSIDIARGEYVAVMGPS
                     GSGKSTFMNVLGCLDRPSAGEVWLDAQPVSALSDDALAAVRNRSIGFVFQSFNLLART
                     SALENVELPLVYAGVAARSRRERALEMLARVGLAERAHHLPQQLSGGQQQRVAIARAL
                     VTRPVLILADEPTGALDSHTSLEIMALLQELNDLGMTVVLVTHEADVARFARRVLRFL
                     DGRVVADEANAAPADARRLGPRDAAVAA"
     gene            1698..2930
                     /locus_tag="AC16O9_06510"
     CDS             1698..2930
                     /locus_tag="AC16O9_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516303.1"
                     /translation="MSLIALLRLALRALAVNKLRSALTMLGIIIGVAAVIVMIAVGAG
                     AQARVEQQIRSLGANLLLILSGARTQGGVRLGVGSNYTLSEEDALAINREIAEALAAP
                     ALRGGAQVVWGNSNWATQIYGTTPDYLEVRQWPLAQGRIFEPAEMAGAGKVCVVGQTV
                     VRQLFASADPVGQVIRIKRVPFTVVGVLETKGQSLMGSDQDDVILVPIATARSRVLGS
                     ASLAKQSAVSTIWVKVADGYDTKVVEEQVRALLRQRHRLQPGAEDDFSLRNLAEFMAA
                     QEAASRVLALLLAAVASVSLVVGGIGIMNIMLVSVTERTREIGLRMALGARTRDILGQ
                     FLVEAVTLSLIGGLVGVLIGVGGALAAARIADWQLVLPGGAVALAVAFAFAIGVFFGF
                     YPARKAARLNPVEALRFE"
     gene            2927..4561
                     /locus_tag="AC16O9_06515"
     CDS             2927..4561
                     /locus_tag="AC16O9_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516304.1"
                     /translation="MSRGRLRHAGCALAVAALAAALYVPFLGNPLVFDDKIFFTGWRF
                     SYYATHPLGFDLRLPPYFSLTMTHALEGGIVAQRVVSLILHIGCALVLYRLLLDLQRA
                     TVRPTNDADAALRAGIGAAAFALHPAAVYGAAYLVQRSIVMATLFGLASAVLFLRGLR
                     RGSHADAISAALLYSLAVLSKEHAVLIPAAVVPVLVLAQAERRFALRHAVIYLAACAP
                     AAIFVTLLSTGVIGGVYEEGAAAIAAQVSAAGGEDILQRSLALSAVTQAGLFFKYLGL
                     WLWPDPGAMSIDLRVDFAAGWSVAWIVAKVAGFIAFGALGAALLLRRGALGLVGFGAL
                     YAWLLFFVEFTAVRYQEPLVLYRSYLWGPGVICMVVAGLSRLPRKGALAAGLAASLAL
                     AWGAHDRLVTFSNPLLLWEDAAAKLPESPVPWGSRTLYGLGREYLYAGQPDKAIAITE
                     RCAQLYPKTVQCVYARGAIHLHLEQYELARQHLQRALDLKPEDGVIHHRLGLALEGLE
                     RLDEAKAAYRRAGEFGYRAGQIELQRLESTGSGRRK"
     gene            complement(4606..5073)
                     /locus_tag="AC16O9_06520"
     CDS             complement(4606..5073)
                     /locus_tag="AC16O9_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR6516305.1"
                     /translation="MKRMQQGFTLIELMIVVAIIGILAAVALPAYQDYTIRAKMSEVI
                     LAMSACRTSITEVYQSGGTPPAANQWGCEAVASKYVGGLATDVNGVITATVQNISPDV
                     NGSLVTLAPLITATQVADTTNNMGQGLFGWRCGGTADGTSVQAKYLPGSCRGN"
     gene            5255..7600
                     /locus_tag="AC16O9_06525"
     CDS             5255..7600
                     /locus_tag="AC16O9_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516306.1"
                     /translation="MPPPLPSWIFPRPRSPRARADALIEKGLRSEKQGNLRAAATHFR
                     AATAAAPDYPTAHVNLGVVLEDLGEADAAAAAYEAALALDPRHPYASYNLGKALLLRG
                     EPARAEALLRAALDAKPGFAEAMVVLASLLDARGDAAGALQSLQAAVAQRPGYALALR
                     NLGMQLCRLQRWAEAAGALEQAVAAEPGNADAHYWLGNARVRLHQPREAEAAYRAALE
                     RRPEFAEAWCNLGNVLADRGLRDEAGQCLERALRIRPQYADALLGMGNLYAATGRLEV
                     AADCYRRALALDPALADAEVNLGNVLKDQGLWREALQHYDAALALSPEAVEARWARAM
                     CHIPAMREAQDDLAALRAGFAAELEHLERWFEGERVDQGWKAVGVAQPFWLAYQEADN
                     RELLRRYGRLCAQLMASWQARQAPPPGPRRAARPVRVGIVSQFFRDHSVWNAIIKGWF
                     RHLDRDGFELQAFCLDPYEDAETRYALARAARFERGHAGLRQWAAAIREARPDVLIYP
                     EIGMDPMSVKLASMRLAPLQAVSWGHPETSGLPTIDAYLSAADLEPPEAQAHYTERLI
                     ALPNLGCCVQPGAVEAAAPGLSREPGVPLLVCPGTPFKYAPEHDGVLAEIAHRLGGRC
                     RLVFFEYRTRALSAALRARLAAGFARRGLDFERCAVFLPWQSRAAFLGLLRSADVFLD
                     TLGFSGFNTAQQAVECGLPIVTREGRFLRGRLASGILKRIGLPALVAADAAQYVDLAV
                     RLAQDPDYRQDITRQIEAGRPALFEDTAPVRALEGFLAQGV"
     gene            complement(7588..9231)
                     /locus_tag="AC16O9_06530"
     CDS             complement(7588..9231)
                     /locus_tag="AC16O9_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR6516307.1"
                     /translation="MSRTLDSTQILGFSAAGRTAADDLVQRLEQLHAIGASLSAERDI
                     HRLLEAILTASKSITRADGGTLYRLTEEGTLRFEIVRTSSLKLYLGGTTGEPIPFYPI
                     HLTGKDGKPNHSMVAAYAALTGKTVNIADAYTADGFDFTGTRNFDKKTGYRSKSFLTV
                     PMMNHEREIIGVLQLINAQDPTTGEVVPFSPADQRLAESLASQAAIALTNRMLINQLE
                     QLFESFIALINTAIDEKSPYTGGHCQRVPALTMMLAEAVSDTTEGPLRDFQMTEKDRY
                     ELKIAGLLHDCGKVTTPVHVVDKATKLEGIYDRVHLIDTRFEILKRDAEIAMLKDGTH
                     EGRARYRERLRQIEEDRMFLRAANIGGERMRDADVERIRRIAEYRWTDVSGHEAKFLS
                     NDELKNLSIRSGTLTAEERQIINHHIVATIKMLEALPWPRHLKNVPEYAGGHHERMDG
                     KGYPKGLTREQMSWQARVMGIADIFEALTAKDRPYKKGKTLSESLEILGRMSLNGHID
                     PELFDVFVRRKIYLRYADMFLDKEQIDDVDPARIPGYTP"
     gene            complement(9254..11617)
                     /locus_tag="AC16O9_06535"
     CDS             complement(9254..11617)
                     /locus_tag="AC16O9_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6516308.1"
                     /translation="MSAVLSPHAAPQTDVSVRLAFQKQLQAVTNKIHATSNIDEIMLE
                     VSADICVLFNADRLTIYSISEDKQAIVSKVKTGLNSFKDLKLPIAEHSIAGYVALAKK
                     TVNLKDVYDDAELKALNPNLRFLQEVDKRTGYRTKQMLVAPIVDGQELIGVIQIINNK
                     AGVPFGPLTEDGVGELSQTLAIAFRQRQKPQLVKTKYDYLISDAVLSAGEFELASRQA
                     RKKAVDVEAVLAEEFQVKIPAIGAALSKFFGVPYEPYKQDRVKPMELLKNLKREYVEQ
                     NQWLPIDDTKEGLVVVCLDPERIRSSRIASNVFPKAKIVYRVTTQREFKETVNLFYGA
                     ESVDMGDIGDMLSSLDDEADGAMEGVTADEASAAADNELVKLVNKIIVDAYNQGASDI
                     HIEPYPGKVKTEIRFRRDGDLGPYIEVPASYRSALAARIKIMCDLDISEKRRPQDGKI
                     KFKKFGPLDIELRVATIPTAGGVEDIVMRILAAGEPMPLDKMGFTKTNEGKLKDTVSK
                     PYGLFFVCGPTGSGKTTTLHSVLKFLNTPETKIWTAEDPVEITQKGLRQVQVNKKAGL
                     DFAAVMKSFLRADPDIIMVGEMRDKETTSTGIEASLTGHLVFATLHTNSAPESITRLL
                     DMGMDPFNFSDALLGILAQRLARRLCSCKQTYTPESAELTAFLREYCDELQNTARFKA
                     DPKGAMEAVYKDWVRHYGNDKGQLTFYKPVGCDKCGGSGFKGRCGLHELLIASDRLKK
                     AIQEHARVAEMLAICLEEGMRTLKQDGMEKCLMGVTHLKEVRSVCIK"
     gene            complement(11742..12509)
                     /locus_tag="AC16O9_06540"
     CDS             complement(11742..12509)
                     /locus_tag="AC16O9_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6516309.1"
                     /translation="MRLKVLGCSGGIGGRHMRTTSFLVDGDVLLDAGTGVGDLSLAEL
                     SLVDHIFVTHSHLDHVASIAFIADTVGGMRSKPILVHALPETIAILKDHIFNWEIWPD
                     FTQIPTPEAPFMRYEEIALGRTVTLAGRAFTPLPAIHTVPAVGFRLDSGSGSLVFTGD
                     TGPNDALWKIVNGIDNLKFLIIETAFADKDRQLAELSQHLCPSMLAEELVKFELDAEV
                     YVTHLKPGETELTMQEIEESSGRLQPRMLQNNQVFEF"
     gene            complement(12516..14735)
                     /locus_tag="AC16O9_06545"
     CDS             complement(12516..14735)
                     /locus_tag="AC16O9_06545"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR6516310.1"
                     /translation="MKQNLVRIVLGLVITLFFIGHAARYYEVPFITRLDNIMYDARLQ
                     VTMPRGVDERVVILDIDEKSLQEVARWPWPRDIMADLVEKLFTTYDVGIVAFDVVFAE
                     ADHSSGIRALDQLAQGQLKDVPSFLESYRDLRPRLDNDGRFAQAIKGKPVVLGYYLNS
                     EQDAKRIAAIPEPVLPKGTFQGRNIPFTTWVGYGGNLVQFTKNAASAGHFNPITDPDG
                     VVRRVPMLAELDGAYYEALSLAVVRTILGFPKVEPGYAPDRFLQRGYTGLEWLQVGPL
                     AIPVDETASALIPYRGRKGSFAYVSLADVLAERVPAERLKGKIALIGTTAPGLLDLRS
                     TPVDNVYPGVEVHANLIAGMLDRNIKLKPPYMLGAEVVLLLVTGVALSLLIPILAPLW
                     ATLATLASMMAITGLNVVVWTEAGMVLPLAASLLMTLSLYTVNMAYGYFVEARSKRQF
                     TELFGQYVPPELVDRMARDPQKYNMEPRAAELTILFSDVRGFTSISEALKPEELREYI
                     NEYLTVMSGIIRSRHHGTLDKYIGDAIMAFWGAPVDDAQHARNGVLAALDMQKECRVL
                     NEKFAARGWPTLKIGVGVNTGNVRVGDMGSRVRRAYTVIGDPVNVASRLEGRTKGYGV
                     GTLVGEATRRHVKDVVFREVDRIKVKGKDEAVTIYEPLGLEGEVERTALDELKLWNPA
                     LRAYRAQQWDQVEVALLNLQRMSPDCGLYQVYADKVAEKRRNPPPPDWDGVAVFDEK"
     gene            complement(14791..>15100)
                     /locus_tag="AC16O9_06550"
     CDS             complement(14791..>15100)
                     /locus_tag="AC16O9_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6516311.1"
                     /translation="PAAAAPAAAAPKSPHPLGAIQILSGGNAGKELELAKPLTTLGKP
                     GVQVAVLTRRPQGYFITHVEGANPPQVNGQAIGTAPHALKDHDVVELAGVKMEFFLKG
                     "
BASE COUNT         2169 a         5119 c         5205 g         2607 t
ORIGIN      
        1 ggtgaagcaa cgcgagtcgc tgctcaagca ggcgcaggtg gacctcgagc gcaccatcat
       61 ccgcgcaccg gtggacggca ccgtgatcct gcgcaacgtg gacgccggcc agaccgtggc
      121 cgcgagcctg caggcgccgg tgctcttcac catcgcgcgc gacctgcgcg acatgcaggt
      181 cgaggcggcg atcgacgaag cggacgtcgg tcggctgcgt cccggcctgc cggcgacgtt
      241 caccgtcgac gcgttcccgc ggcgcggttt cagcggcgag atcgtgcaaa tccgcaagtc
      301 tccggtgaac gtgcagaacg tggtcagcta tacggtggtg atctcggcgc gcaatccgga
      361 cctcgcgctg ctgccgggca tgacggccaa cgtgcgcatc gtggtcgagc agcgcgacaa
      421 cgcgctcaag gtgccgaatg ccgcgctgcg cttccggcct gccggcgccg ccgagccggc
      481 gcccgccgcg gcagcgccgc agccgggcgg tggccaggcg ctgcagcagt tccgccagcg
      541 gctcgtcgag gccgtgcagc cggacgcctc gcagcgcgcg caactggagg agatcttcaa
      601 cgacctgcgc cggaaggcgg cgcccctgcg tgagctggcc gacgaggcgg agcggcgccg
      661 ccagtccgag cgcctgcgcg ccgacatgcg cacccgcgtg gccgagattc tcaagcccga
      721 gcagcgcgcc gcctatgagc agctgctgcg cgactacgcc ggccgcgcag cggtggcaac
      781 cggccgagtc tgggtgccgg acggccgcgg cacccgtgcc atcgacgtgc ggctcggcct
      841 aagcgacggc ctgaatactg aaatcgtcgc cggcgagctc aaggaaggcg ccgaggtcat
      901 cgtcggattg gcggcgaccg agcagcgcgg caccggcctg ccgcgccttc gactcttctg
      961 atgggcgagc cgctgatccg cacgcgcgac gtggccaagg agtaccgcgt cggcgacaac
     1021 ctgatccgcg cattggccgg ggtcagcatc gacatcgcgc gcggcgagta cgtcgccgtg
     1081 atgggaccct caggctcggg caagtcgacc ttcatgaacg tgctcggctg cctcgaccgg
     1141 ccgagcgcgg gcgaggtgtg gctcgacgcc cagcccgtat ccgcgctgag cgacgatgcg
     1201 ctggctgcag tccgcaaccg cagcatcggc ttcgtgttcc agagcttcaa cttgcttgcg
     1261 cgcaccagcg cgctggaaaa cgtcgagctg cccctggtct acgcgggcgt cgctgcccgg
     1321 tcgcggcgcg agcgcgccct cgagatgctg gcgcgcgtcg gcctggcaga gcgcgcgcac
     1381 cacctgcccc agcagctctc cggcggccag cagcagcgcg tcgccatcgc gcgcgccctg
     1441 gtgacgcgcc cggtgctgat cctggcggat gaaccgaccg gcgccctcga ttcccacacc
     1501 agcctcgaga tcatggcgct gctgcaggaa ctcaacgacc tgggcatgac cgtggtgctc
     1561 gtgacgcacg aggcggacgt ggcgcgcttt gcgcgccgtg tgctgcgttt cctggacggg
     1621 cgcgtcgtgg cggacgaagc caacgcagcg cccgcagacg cgcggcgcct gggcccccgg
     1681 gacgcggcgg tggccgcatg agcctcatcg cgctgctgcg cctggcgctc agggctctgg
     1741 cggtgaacaa gctgcgcagc gccctcacca tgctcggcat catcatcggc gtggccgcgg
     1801 tgatcgtcat gatcgcggtc ggcgcgggcg cgcaggcccg ggtcgagcag cagatccgct
     1861 cgctcggcgc gaacctgctg ctcattctct ccggcgcacg cacccagggc ggcgtgcgtc
     1921 tcggcgtggg ctccaactac acgctctcgg aggaagacgc cctcgccatc aaccgcgaga
     1981 tcgccgaggc gctcgcagcg ccggcgctgc gcggcggcgc gcaggtggtc tggggcaaca
     2041 gcaactgggc cacgcagatc tacggcacca cgccggacta tctcgaggtg cggcagtggc
     2101 cgctggcgca ggggcgcatc ttcgagcccg cggagatggc gggcgcgggc aaggtatgcg
     2161 tggtcggcca gacggtggtg cggcagctgt tcgccagcgc cgatccggtg ggccaggtga
     2221 tccgcatcaa gcgcgtcccc tttaccgtgg tcggcgtcct cgagaccaag ggccagagtt
     2281 tgatggggag cgaccaggac gacgtgatcc tggtgccgat cgccacggcg cgcagccgcg
     2341 tgctcggcag cgccagcctc gccaagcaga gcgccgtcag caccatctgg gtgaaggtcg
     2401 ccgacggcta cgacaccaag gtcgtcgagg agcaggtgcg cgccctgctg cgccagcgcc
     2461 atcgcctgca gccgggcgcg gaagacgact tttcgctgcg caacctggcc gagttcatgg
     2521 cggcccagga ggccgcgagc cgggttctcg ccctgctgct cgccgcggtg gcgtcggtgt
     2581 cgctggtggt cggcggcatc ggcatcatga acatcatgct cgtgtcggtc accgagcgca
     2641 cacgcgagat cgggctgcgc atggcgctcg gcgcgcgcac gcgcgacatc ctgggccagt
     2701 tcctggtcga ggcagtgacg ctgtcgctga tcggcggatt ggtgggcgtg ctgatcggcg
     2761 tgggcggcgc gctggccgcc gcgcggatcg ccgactggca gctggtcctc ccgggtgggg
     2821 cggtggcgct cgcggtggcg ttcgcgttcg cgatcggcgt gttcttcggc ttctatccgg
     2881 cgcgcaaggc ggcccgcctc aacccggtgg aagcgctgcg cttcgaatga gccgcgggcg
     2941 cctccggcac gctggatgcg cgctcgccgt ggccgcgctg gccgccgccc tgtacgtgcc
     3001 gttcctcggc aatccgctgg tgttcgacga caagatcttc ttcaccggct ggcgattctc
     3061 ctattacgcc acgcacccgc tcggcttcga cctgaggttg ccgccgtatt tttccctcac
     3121 gatgacgcac gcgctcgagg gcggcatcgt cgcccagcgc gtggtcagcc tgatcctgca
     3181 catcggctgc gccctcgtcc tgtacaggct cctgctcgac ctgcagcgcg cgacggtgcg
     3241 tccgaccaat gacgccgatg ccgcgctgcg cgcggggatc ggtgcggccg cgttcgcctt
     3301 gcacccggcg gcagtctatg gcgccgctta cctggtccag cgcagcatcg tcatggcgac
     3361 gctgttcggg ctcgcctcgg cggtgctctt cctgcgcggg ctgcgccgcg gcagccatgc
     3421 cgacgcgatt tccgccgcgt tgctctattc gctggcggtg ctttccaagg agcacgccgt
     3481 cctgataccg gctgcggtgg tcccggtgct cgtgctggcg caggccgagc ggcgcttcgc
     3541 gctgcggcat gccgtgatct atcttgccgc ctgcgcgccg gcggccatct tcgtcaccct
     3601 gctgagcacg ggcgtgatcg ggggcgtcta cgaggaaggc gccgcagcca tcgcggcgca
     3661 ggtgagcgcg gccggcggcg aggacatcct gcagcgctcg ctcgcgctca gcgcggtgac
     3721 gcaggccgga ttgttcttca agtacctcgg cttgtggctc tggccggacc ctggggcgat
     3781 gtcgatcgac ctgcgcgtgg acttcgccgc cggctggagc gtcgcctgga tcgtggcgaa
     3841 ggtcgcggga ttcatcgcgt tcggggccct cggtgcggcg ctgctcctgc gccgcggcgc
     3901 cctgggcctc gtgggtttcg gcgcgctcta cgcgtggctg ctgttcttcg tcgagttcac
     3961 cgccgtgcgc tatcaggaac cgctcgtttt gtaccgcagc tacctgtggg gcccgggggt
     4021 gatctgcatg gtggtagccg ggctatcgcg cctgccgcgc aagggggcgc tcgcagccgg
     4081 cctcgccgcc agcctcgcgc tcgcctgggg cgcgcacgat cggctggtca ccttctcgaa
     4141 ccccttgttg ctgtgggagg acgcggcggc gaagcttccc gagtcgcccg tgccctgggg
     4201 ctcgcgcacg ctctacggcc tgggccgcga gtacctgtac gccggccagc cggacaaggc
     4261 cattgcgatc accgaacgct gcgcgcagct ttatccaaag accgtgcagt gcgtctacgc
     4321 gcgcggcgcc atccacttgc acctcgagca atacgagctg gcgcggcagc atttgcagcg
     4381 cgcgctcgat ctaaagcccg aggacggcgt catccaccac cggctggggc tcgcgttgga
     4441 aggcctggaa cgcctggacg aagcgaaagc cgcctaccgg cgcgccggcg aattcggcta
     4501 cagggcgggg cagatcgagc tgcagcggct cgagagcact ggcagcgggc gcaggaaata
     4561 aaaaaggcgc cccgcagggc gccttcttcg tgctgccgct gccgcttagt tgccgcggca
     4621 agaacccggc aggtacttgg cctggacgct cgtgccgtcc gcggtaccac cgcagcgcca
     4681 gccgaacagg ccctggccca tgttgttggt ggtatccgcg acctgcgtcg ccgtgatgag
     4741 cggcgcgagg gtcacgaggc tgccgttcac gtccgggctg atgttctgca ccgtcgccgt
     4801 gatcacgccg ttcacgtcgg tcgccaggcc acccacgtac ttcgaggcca ccgcttcgca
     4861 gccccactgg ttggcggcgg gcggcgtgcc acccgactgg tacacctcgg taatcgacgt
     4921 acggcaggcg ctcatcgcca gaatcacctc ggacatcttc gcccggatgg tgtagtcctg
     4981 gtacgcgggg agcgccaccg cggccagaat gccgatgatc gcgaccacga tcatcaactc
     5041 gatcagcgtg aagccttgtt gcatgcgttt cattcgactc tcctttgagg taacgacgcg
     5101 gcggtgccgc tggcccctat ctctgcaagg cgcgtgccag gccgtggctg ccaaaaacct
     5161 caccaaattg ggggcttagc gggcctcaag gtgacggaaa tgggcagtcc ctgaccgatc
     5221 ttggaccacg ccggggctat gtgaaaatcg tcacgtgccc ccgccgctac cgagctggat
     5281 cttcccgcgc ccgcgtagcc cccgggcccg ggctgacgcg ctgatcgaaa agggcctgcg
     5341 cagcgaaaag caggggaatc ttcgagccgc tgccacgcat ttccgtgctg cgacggcggc
     5401 cgcgccggac tatcccaccg cacacgtcaa tctgggtgtc gtgctggagg atctgggcga
     5461 ggcggacgct gccgcggcag cctatgaagc agcgcttgcg ctggatcccc gccaccccta
     5521 cgcgagctat aacctcggca aagcgctgct gctgcggggg gagcccgcgc gggccgaggc
     5581 gttgctgcgc gctgcgctcg acgccaagcc gggttttgcc gaggccatgg tggtgctggc
     5641 cagcctgctt gacgcgcggg gcgatgcggc cggcgcgctg cagtcgctgc aggccgccgt
     5701 cgcgcagcgc cctggctacg cgcttgcgtt gcgcaacctc gggatgcagt tgtgccggct
     5761 gcagcgctgg gcagaagccg cgggcgccct ggagcaggcg gtggccgccg agccgggaaa
     5821 tgcagacgcg cactactggc tcggcaacgc gcgggtgcgg ttgcaccagc ccagggaagc
     5881 ggaagccgcg taccgtgccg cgctcgagcg ccggcccgag tttgccgaag cctggtgcaa
     5941 cctgggcaat gtgctcgccg accgcgggct gcgcgacgaa gccggccagt gcctggagcg
     6001 cgccctgcgg atccgccccc agtacgccga cgccctgctc gggatgggaa acctctacgc
     6061 cgccaccggg cgcctggagg tggcggccga ttgctaccgc agggcgctgg cgctcgatcc
     6121 cgcgctcgcc gacgccgaag tcaacctcgg caacgtgctc aaggaccagg gcctgtggcg
     6181 cgaagcgctg cagcactacg acgccgcgct ggcgctcagt cccgaggcag tggaggcgcg
     6241 gtgggcgcgc gccatgtgcc atatcccggc gatgcgcgag gcgcaggacg atcttgccgc
     6301 tttgcgtgcc ggctttgctg cggagctcga gcatctggag cgctggttcg agggcgagcg
     6361 cgtcgaccaa ggctggaagg ccgtcggcgt ggcgcagccg ttctggctgg cctatcagga
     6421 ggcggacaac cgggagctgc tacgccgcta tgggcggctg tgcgcgcagc tcatggcgtc
     6481 ttggcaggcg cgtcaggcgc cgccgcccgg cccgcgccgc gccgcgcgcc ctgtccgggt
     6541 cggcatcgtg tcgcagttct tccgcgacca ctcggtgtgg aacgccatca tcaaaggctg
     6601 gttccggcat ctggatcgcg acggattcga gctgcaggcg ttctgcctcg acccctacga
     6661 ggacgccgag acgcgctacg ccctcgcgcg ggccgcgcgc ttcgagcgcg ggcacgcggg
     6721 actgcgccag tgggccgctg cgatccggga ggcccggccc gacgtgctga tctatcctga
     6781 gatcggcatg gatccgatga gcgtgaagct ggcgagcatg cgccttgcgc ccttgcaggc
     6841 ggtgtcttgg ggccacccgg aaacgagcgg cctgccgacg atcgacgcct acctctcggc
     6901 ggccgacctc gagccgccgg aggctcaggc gcattacacc gagcggctca tcgcgctgcc
     6961 caacctcggc tgctgcgtgc agccgggcgc ggtcgaggcc gccgcgccgg gcttgagccg
     7021 ggaaccgggc gtgccgctgc tcgtgtgccc cggcacgccg ttcaagtacg cacccgagca
     7081 cgacggagtg ctggccgaga tcgcgcaccg gctcggcggc cgctgccggc tggtgttctt
     7141 cgagtaccgc acgcgcgccc tgtcggcggc actgcgcgcg cggctcgccg ccgggttcgc
     7201 ccgtcgcggc ctggatttcg agcgctgcgc cgtatttctc ccctggcaga gccgcgccgc
     7261 gtttctcggc ttgttgcgca gcgcggacgt attcctcgac acgctcggct tctcgggctt
     7321 caacaccgcg cagcaggctg tcgaatgcgg ccttccgatc gtgacgcgcg aagggcgctt
     7381 tctgcgcggc cggctggcga gcgggatcct gaagcgcatc ggcctgccgg cgctggtggc
     7441 cgccgacgcg gcgcaatatg tggatctggc ggtcaggctg gcgcaggacc cggactaccg
     7501 acaggacatc acgcggcaga tcgaggccgg ccggccagcg ctattcgaag acaccgcgcc
     7561 ggtgcgggcc ctcgaaggct tcctcgctca gggcgtatag ccggggatcc gcgccgggtc
     7621 gacgtcgtcg atctgctcct tgtcgagaaa catgtcggcg tagcgcaggt agatcttgcg
     7681 gcgcacgaac acatcgaaga gctccggatc gatgtgcccg ttgaggctca tgcgccccag
     7741 gatctccaga gactccgaca gcgtcttgcc cttcttgtag ggccggtcct tggcggtcag
     7801 cgcctcgaaa atatcggcga tgcccatgac gcgcgcctgc cacgacatct gctcgcgcgt
     7861 caggcccttg ggatagccct tgccgtccat gcgctcgtgg tgcccgcccg cgtactcggg
     7921 cacgttcttc aggtgccgcg gccagggcag cgcctccagc atcttgatgg tggcgacgat
     7981 gtggtggttg atgatctgcc gctcctcggc ggtgagcgtg cccgagcgga tgctgaggtt
     8041 cttcagctcg tcgttgctga ggaacttggc ctcgtggccg gagacgtcgg tccagcggta
     8101 ctcggcgatg cgccggatgc gctcgacgtc cgcgtcgcgc atgcgctcgc cgccgatgtt
     8161 ggcggcgcgc aggaacatgc ggtcctcctc gatctggcgc agccgctcgc ggtagcgcgc
     8221 gcgcccctcg tgggtgccgt ccttcagcat cgcgatctcg gcgtcgcgct tcaggatctc
     8281 gaagcgcgtg tcgatgaggt gcacgcggtc gtagatgccc tcgagcttgg tggccttgtc
     8341 caccacgtgc accggcgtgg tcaccttgcc gcagtcgtgc agcaggcccg cgatcttgag
     8401 ctcgtagcgg tccttctcgg tcatttggaa atcgcgcagc gggccctcgg tggtgtcgct
     8461 caccgcctcg gccagcatca tggtgagcgc gggcacgcgc tggcagtggc cgccggtgta
     8521 gggcgacttt tcgtcgatcg cggtattgat gagcgcgatg aacgactcga acagctgctc
     8581 gagctggttg atcagcatgc ggttggtgag cgcgatcgcc gcctgcgagg cgagcgattc
     8641 ggccagccgc tggtcggcgg gcgagaacgg caccacctcg cccgtcgtcg ggtcctgggc
     8701 attgatcagc tgcagcacgc cgatgatctc gcgctcgtgg ttcatcatcg gcacggtgag
     8761 gaaggacttc gagcggtagc cggtcttctt gtcgaagttg cgcgtgccgg tgaaatcgaa
     8821 gccgtcggcg gtataggcgt ccgcgatgtt caccgtcttg ccggtcagtg cggcgtacgc
     8881 ggccaccatg ctgtggttcg gcttgccgtc cttgcccgtg aggtggatcg gatagaacgg
     8941 aatcggctcg ccggtggtgc cgccgaggta gagcttgagg ctcgaggtgc gcacgatctc
     9001 gaagcgcagg gtgccctctt ccgtcaggcg gtagagcgtg ccgccatcgg cgcgcgtgat
     9061 cgacttggag gcggtaagga tggcctccag cagccggtgg atgtcacgct ccgcggacag
     9121 cgaagcgccg atagcgtgca gctgctcgag gcgctgcacc aggtcgtcgg ccgcagtgcg
     9181 ccccgcggcc gagaagccca gtatctgggt gctgtcgagg gtgcggctca tgcgctcgcc
     9241 aggcggcgcc gcctcacttg atgcagaccg agcgcacttc cttcaggtgc gtgactccca
     9301 tcaggcactt ctccatcccg tcctgcttca gcgtgcgcat gccctcctcc aggcagatcg
     9361 ccagcatctc ggccacgcgc gcgtgctcct ggatcgcctt cttcaggcgg tccgaggcga
     9421 tcagcagctc gtgcaggccg cagcgtcctt tgaagccgct gccgccgcac ttgtcgcagc
     9481 ccacgggctt gtagaacgtg agctgaccct tgtcgttgcc gtagtgccgc acccagtcct
     9541 tgtacacggc ttccatcgcg cccttcgggt cggccttgaa gcgcgccgtg ttctgcagct
     9601 cgtcgcaata ctcgcgcagg aacgcggtca gctcggccga ctcgggcgta taggtctgct
     9661 tgcagctgca caggcgccgg gcgaggcgct gcgcaaggat gccgagcagc gcgtccgaga
     9721 agttgaaagg gtccatgccc atgtcgagca agcgggtgat ggattccggc gccgagttgg
     9781 tgtgcagcgt ggcgaacacg aggtggccgg tgagcgaagc ttcgatgccg gtcgaggtgg
     9841 tctccttgtc gcgcatctcg ccgaccatga tgatgtccgg atcggcgcgc aggaacgact
     9901 tcatcaccgc ggcgaagtcc aggcccgcct tcttgttcac ctgcacctgg cgcaagccct
     9961 tctgcgtgat ctcgaccggg tcctccgcgg tccagatctt ggtttccggc gtgttgagga
    10021 acttgagcac cgagtgcagc gtcgtggtct tgcccgagcc ggtcgggccg cagacgaaga
    10081 acaggccgta gggcttgctc accgtgtcct tcagcttgcc ctcgttcgtc ttggtgaagc
    10141 ccatcttgtc cagcggcatc ggctcgccgg cggcgagaat ccgcatcacg atgtcctcga
    10201 cgccgccggc cgtgggaatc gtcgccacgc gcagctcgat gtcgagcggc ccgaacttct
    10261 tgaacttgat cttgccgtcc tgcggacggc gcttctccga aatatccagg tcgcacatga
    10321 tcttgatgcg cgcggcgagc gccgaccggt agctcgcggg cacctcgatg taagggccca
    10381 ggtcgccgtc gcggcggaag cggatctcgg tcttcacctt gccggggtac ggctcgatat
    10441 ggatgtcgga cgccccctgg ttgtaggcat cgacgatgat cttgttgacc agcttcacca
    10501 gctcgttgtc ggcggcggcg gaggcctcgt ccgccgtcac cccctccatc gccccgtcgg
    10561 cctcgtcgtc gaggctcgac agcatgtcgc cgatgtcgcc catgtccacg gactcggcgc
    10621 cgtagaacag gttgacggtt tccttgaact ccctctgcgt cgtgacacgg tagacgatct
    10681 tcgccttcgg gaacacgttg gaggcgatgc gcgagctgcg gatgcgctcc gggtccaggc
    10741 acaccaccac caggccttcc ttggtatcgt ctatcggcag ccactggttc tgctcgacgt
    10801 actcgcgctt caggttcttc agcagctcca tcggcttcac gcggtcctgc ttgtagggct
    10861 cgtagggcac gccgaagaac ttcgacagcg ccgcgccgat cgccgggatc ttgacctgga
    10921 attcctcggc gagcaccgcc tcgacgtcga cggccttctt gcgcgcctgg cgcgaagcga
    10981 gctcgaactc gcccgccgag agcaccgcgt ccgagatcag gtagtcgtac ttggtcttga
    11041 cgagctgcgg cttctgccgc tggcggaaag cgatcgccag cgtctgcgac agctcgccga
    11101 cgccgtcctc ggtcagcggg ccgaacggca cgccggcctt gttgttgatg atctggatga
    11161 cgccgatcag ctcctggccg tccacgatcg gcgccaccag catctgcttg gtgcggtagc
    11221 cggtgcgctt gtcgacttcc tgcaggaagc gcaggttcgg gttgagcgcc ttcagctcgg
    11281 cgtcgtcgta gacgtccttc aggttgaccg tcttcttggc gagcgcgacg tagcccgcga
    11341 tcgagtgctc ggcgatcggc agcttcaggt ccttgaacga gttgaggccc gtcttcacct
    11401 tggagacgat ggcctgcttg tcctcgctga tcgagtagat cgtcaggcgg tcggcgttaa
    11461 acagcacgca gatgtccgcg ctcacctcga gcatgatctc gtcgatgttg ctggtggcgt
    11521 ggatcttgtt ggtgaccgcc tgcagctgct tttggaacgc gagccggacg ctgacgtcag
    11581 tctgcggcgc agcgtgtggg ctgagaacgg cgctcatcgt ggacctctcg attcgggctt
    11641 gccggctatg ctatcgtccg gtgcgcaaac attcaaccgc cgccgaccgc caccgccctg
    11701 atttttaggc ggtttttcac gtccgacggc tccgcgcgcg gtcaaaattc aaaaacctgg
    11761 ttgttctgca gcatgcgcgg ctgcagccgg cccgaggact cctcgatctc ctgcatcgtg
    11821 agctcggtct cgccgggctt gaggtgggtg acgtacacct cggcgtcgag ctcgaatttc
    11881 actagctcct cggcgagcat cgaggggcac aggtgctgcg agagctcggc gagctggcga
    11941 tccttgtcgg cgaacgcagt ctcgatgatg aggaatttca ggttgtcgat gccattcacg
    12001 atcttccaca gcgcgtcgtt cgggccggta tcgccggtga acaccaggct gccgctgccg
    12061 ctatcgagcc ggaagccgac cgccggcacc gtgtggatcg ccggcagcgg cgtgaacgcg
    12121 cggcccgcga gcgtcaccgt gcggcccagc gcaatttcct cgtagcgcat gaatggcgct
    12181 tcgggcgtcg ggatctgcgt aaagtccggc cagatctccc agttgaagat gtgatccttc
    12241 aggatggcga tggtctcggg cagcgcgtgc accaggatcg gcttggagcg catgccgccg
    12301 acggtgtcgg cgatgaacgc gatcgaggct acgtgatcca ggtgcgaatg ggtgacgaag
    12361 atgtggtcga ccagcgacag ctcggccagc gacaggtcgc ccacgcccgt gcccgcgtcg
    12421 agcagcacgt cgccgtcgac caggaacgag gtggtgcgca tgtgccgccc gccgatgccg
    12481 ccggagcagc cgagaacctt gagcctcata cgcgcctatt tctcgtcgaa caccgcgacg
    12541 ccgtcccagt ccggcggcgg cgggttgcgg cgcttctccg ccaccttgtc cgcgtacacc
    12601 tggtatagac cgcaatccgg actcatgcgt tgcagattga gcagtgccac ctcgacctgg
    12661 tcccactgct gggcgcggta ggcgcgcagg gccgggttcc agagcttcag ctcgtccagg
    12721 gccgtgcgct cgacctcgcc ttccaagccg agcggctcgt agatggtgac cgcctcgtcc
    12781 ttgcccttga ccttgatccg gtccacctcg cggaatacga cgtccttgac gtgcctgcgc
    12841 gtcgcctcgc cgaccagggt gccgacgccg tagcccttgg tgcggccttc cagccgcgag
    12901 gccacgttca ccggatcgcc gatcacggtg taggcgcgcc gcacccgcga gcccatgtcg
    12961 cccacgcgta cgttgccggt attcacgccg acgccgatct tgagcgtcgg ccacccgcgc
    13021 gcggcaaatt tctcgttgag gaccctgcac tccttctgca tgtcgagcgc cgcgagaacg
    13081 ccgttccttg cgtgctgcgc gtcgtccacc ggcgcgcccc agaacgccat gatggcatcg
    13141 ccgatgtact tgtcgagcgt gccgtggtgg cggctgcgga tgatcccgct catcacggtg
    13201 aggtactcgt taatgtactc gcgcagctcc tccggcttga gcgcctccga gatgctggtg
    13261 aagccgcgta cgtccgagaa caggatcgtg agctcggcgg cgcgcggctc catgttgtac
    13321 ttctgcggat cgcgcgccat gcggtcgacc agctccggcg gcacgtactg gccgaacagc
    13381 tccgtgaact ggcgcttgga gcgcgcctcc acgaaatagc cgtaggccat gttgacggta
    13441 tagagcgaaa gcgtcatcag cagcgacgcg gcgagcggca gcaccatacc ggcctcggtc
    13501 cagaccacca cgttcagccc cgtgatcgcc atcatgctcg ccagcgtggc gagggtcgcc
    13561 cacagcgggg cgagaatcgg gatcaggagc gacagcgcga ccccggtcac cagcagcagc
    13621 accacttccg cgccgagcat gtagggcggc ttcagcttga tgttcctgtc gagcatgccg
    13681 gcaatcaggt tggcgtgcac ttccaccccc ggataaacgt tgtcgacagg cgtcgagcgc
    13741 aggtcgagca ggcccggcgc ggtggtgccg atcagcgcaa tcttgccttt caggcgctcc
    13801 gccggcacgc gctccgcgag cacgtccgcc agcgacacgt aggcgaagct gcccttgcgg
    13861 ccgcgatacg gaatcagcgc gctcgcggtc tcgtccacgg ggatggcgag cgggccgacc
    13921 tgcagccact ccagtccggt gtagccgcgt tgcagaaagc ggtcgggcgc gtagcccggc
    13981 tcgaccttcg gaaagccaag gatcgtgcgc acgacggcga gcgacagcgc ctcgtaatat
    14041 gcgccatcga gctcggcgag catcggcacc cgccgcacca cgccgtcggg atcggtgatc
    14101 gggttgaagt gcccggcgct ggcggcgttc ttcgtgaact ggaccaggtt gccgccgtag
    14161 cccacccagg tggtgaacgg gatgttgcgt ccctggaagg tgcccttggg aagcaccggc
    14221 tccggaatcg cggcaatgcg cttcgcgtcc tgctcactgt tcaggtaata gccgagcacc
    14281 accggcttgc ccttgatcgc ctgcgcgaat cgcccgtcat tgtccagccg cgggcgcagg
    14341 tcgcggtagg actccaggaa gctcggcacg tccttgagct gcccctgcgc aagctggtcc
    14401 agcgcgcgga ttccggacga atggtcggcc tcggcgaaca ccacgtcgaa cgcgacgatg
    14461 ccgacgtcgt aggtcgtgaa cagcttctcg accaggtccg ccatgatgtc gcgcggccag
    14521 ggccagcgtg ccacctcctg caggctcttc tcgtctatgt ccaggatcac gacgcgctcg
    14581 tccaccccgc gcggcatggt cacctgcagg cgcgcgtcgt acatgatgtt gtcgagccgc
    14641 gtgatgaacg gcacctcgta gtagcgcgcc gcgtggccga tgaagaacag cgtgatgacg
    14701 agcccgagga cgatgcggac taggttctgt ttcacgcgcg gtccgtcggg ccggcggagc
    14761 cgcgggcggg gcgcggtgcc gcgccgcggc tcatcccttc aggaagaact ccatcttgac
    14821 gcccgccagc tcgacgacgt cgtggtcctt gagcgcgtgc ggcgcggtac cgatggcctg
    14881 accgttcacc tgcggcgggt tcgcgccctc gacgtgggtg atgaagtagc cctgcgggcg
    14941 gcgcgtcagc accgccacct gcacgcccgg tttgccgagg gtggtcagcg gcttggcgag
    15001 ctcgagctcc ttgccggcgt tgccgcccga caggatctgg atcgcaccaa gcgggtgcgg
    15061 gcttttcggc gccgcggcgg caggggccgc ggcggccggg
//
LOCUS       DBNHKE010000106        14728 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_282860, whole genome shotgun sequence.
ACCESSION   DBNHKE010000106 DBNHKE010000000
VERSION     DBNHKE010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14728
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_282860"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="AC16O9_06555"
     CDS             <1..1826
                     /locus_tag="AC16O9_06555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6516312.1"
                     /translation="ADVVVEETYGFGRHTGVCNEGRAILADYNAGEHSLTVYHATQAP
                     HMMQDIFSRHLNIPEANVRVITKDVGGSYGIKVHVYPDEMATAALSVMLRRPVKFVAD
                     RLESFLSDIHAREHKAKVRLACTKAGEILAFELDDLTPIGPYSVYPRTSGIEGNQVVN
                     LTGGPYKHQKYRAKMHVVFTNKNVTCQYRAVGHPIAVALTEAIVDLAAAKLGMDPAEF
                     RRKNLIPDDAYPYTFPSGIKFEKLSHHQTLDKLLALMDYKKLREEQAALRKKKVYRGI
                     GLAAMIEVTNPSPAFYGVGGARISAQDGATLRLEPTGMVTVMCSVTEQGQGTEGIFAQ
                     IAASAVGVTMDKVRVITGDTGVTPYGGGTWASRGAGIGGEAVLLAGRALRANMIEVAA
                     AILKVDKAELDVQANAVVHKLTQEQKMPLAELGRVGYFRPDTLPPDFQAELTVTRHYT
                     PRQYGFTFTNGIQASLVEVDPDTGLVKLLKHWCVEDCGTVINPMLVDEQIRGGVVQGI
                     GAALYEECLYDASGQLLNGNMADYLVPMALEMPDIVCAHIETPTKQSQLGAKGVGEAG
                     TAGAPGAVMNAINDALAPLNARVTSMPFTPERILRALGKVA"
     gene            1823..2491
                     /gene="maiA"
                     /locus_tag="AC16O9_06560"
     CDS             1823..2491
                     /gene="maiA"
                     /locus_tag="AC16O9_06560"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HIR6516313.1"
                     /translation="MKLYGYWRSLATFRVRVALALKGLRLDQPEIPIDLMQGMQHDAA
                     YRRVNPQAVVPALILEDGGAPLFQSMAILEYLEETHPLPALLPKDARGRARVRGLAQI
                     CVSDGHPFIVPRVRGYLEKTLGLDEAARNRWVAHWSLQALEALEGHLAREPGTARYCH
                     GDAPTLADVCLASQVVAAKDYGRCDVSGVPTVMRIYGECMKQGAFAQSHPARQPGAPA
                     DLRP"
     gene            2571..3803
                     /locus_tag="AC16O9_06565"
     CDS             2571..3803
                     /locus_tag="AC16O9_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6516314.1"
                     /translation="MKRICVALLAFAFAALSGAAAAAEPIKIGFSMALTGGLAGAGKA
                     ALIAMEIWREDVNKKGGLLGRQVEFVYYDDATQPAKVPPIYTKLLNVDKVDLVVSSYG
                     TNEIVPAMPIVMRNKMVFPSLFGLAANEEFKYNRYFQIMPAGPDPKVDWSRGFFELAA
                     QQNPKPKTVAIVAADAEFPINAATGARKNAQAAGLKIVYDKTYPPATTDYTPIVRAIQ
                     ATNADIVYVASYPPDSVGMVKAANEVGLKAKMFGGGMVGLQFAAIQKNLGPLLNGVIN
                     YDFWVPEPTLDFPGVKEFLARYQEAAKDKGVDPLGHYLPPFAYAYMQILGNAVQATKS
                     LDQGKIADYFRKNTHDTVVGKVKFGKNGEWATSRVLMVQFRDVQPDNLEQFAGPGKRV
                     VLYPKAWKSGDIVFPFKQ"
     gene            3836..4708
                     /locus_tag="AC16O9_06570"
     CDS             3836..4708
                     /locus_tag="AC16O9_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516315.1"
                     /translation="MFSADLFANAVVSGLLLGGFYAAVAIGLAIVFGQLDIVNIAHPA
                     FIIAGAYTAYIFNTTFGFDPILVGVAAMPVFFAVGVVIYRIYYAAFERTGQESLSGLA
                     FFFGLMFIIEVSLILVYGVDYRLVSAGYSAQTLAFGFVDLPGRMLIPFLVAMTLTGAV
                     WLYLSRTFMGRAIQAVAQDRGALQLMGADPVRIKQWAFGIGIATAAVAGALLIIIGPV
                     EPSVGRQYIGRAFAIVVLGGMGSIQGMLIAALALGVVENMVSTFYGPSWAPAVAFGVL
                     LATLAFRPSGLFGR"
     gene            4711..5658
                     /locus_tag="AC16O9_06575"
     CDS             4711..5658
                     /locus_tag="AC16O9_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516316.1"
                     /translation="MRGWGFWAASAAALAAGLAFTALADNPYYFNATYVILQALIMAV
                     AWNILGGFTGYVNFGSAAFFAVGAYISIALLKTADVPLPLLILASALVCGLIGLGVGY
                     LTLRLKGVYFAIATLAMGIVLETLVTNWSYVGGAKGTAILTPETHLWFDSYSEMLFMV
                     MLLLALGSVAISRALSSSRIGRGLAAIRDDEVAAECSGVPTLRLKLVSTTVMGAMMGA
                     AGAPYPYFVTYVDPISAFNLFIAVNAIAMPMIGGTAHWAGPVIGALLVGSMQEVITVT
                     ISSELNVLLVGIMLVLFITLAPQGIVGLVRKHWKRRKSA"
     gene            5655..6377
                     /locus_tag="AC16O9_06580"
     CDS             5655..6377
                     /locus_tag="AC16O9_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516317.1"
                     /translation="MSALLQVSGLAKRFGGFTALEGITLAVAPGERLGLIGPNGSGKT
                     TLINCVSGALFNDEGRVEFEGRDVTRLPPFQRARLGIARSFQIPKPFRSMTVLDNLRV
                     PLEYAARTRAGQRSAVDEAMRILETMGLAARARQDSAGLTQVDMRKLELARAMAAHPR
                     LLISDEAMAGLSGAEVDEVLEILFKLNGAGITIIMIEHIMQAVMRFSQRVVCLDAGRI
                     ICEGTPDQTVANPDVQRAYLGA"
     gene            6367..7098
                     /locus_tag="AC16O9_06585"
     CDS             6367..7098
                     /locus_tag="AC16O9_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516318.1"
                     /translation="MALSLTVRGVQAGYGAVRALHGVSLDIQDTETVALLGTNGNGKS
                     TLMKCVMGIVRPTQGSIVLQVDGAEHDLTRLTTEQIVNLGVAMVPEGRRLFPKLTVEE
                     NLLLGAFRPAARSGIAKNLAFTYEAFPVLAERRRQLAGSMSGGQQQMLAIGRALMSAP
                     RLLLVDEPSVGLAPILVSTTITKIKELKDRYGLTVLMAEQNFHQAIRIADRGYIIVHG
                     EIVFDGRSVAELEGNDLVKRYYLGT"
     gene            complement(7007..7648)
                     /locus_tag="AC16O9_06590"
     CDS             complement(7007..7648)
                     /locus_tag="AC16O9_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6516319.1"
                     /translation="MADWKRIRTLSKRAMLKRVARFSRLKGSDGGLPDSPLPECERTL
                     YAVIGFAPPKKGTRATTSPVGDDASAMPAIAIAEGFNLGYCKAKPGKGPLMHNHDTNE
                     TFIPMTGRWRCEWNEGRKKLHADLGPYDVISFPPGVARRFMNVTYAEPKKEHLLMFVI
                     GGNQPRAEFTPAAMRRCEAWGAAVRSRGSSASPDRFPRARRPSARRTRSRRAR"
     gene            7683..8774
                     /locus_tag="AC16O9_06595"
     CDS             7683..8774
                     /locus_tag="AC16O9_06595"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR6516320.1"
                     /translation="MKPPFRADQVGSLLRPAALAEARRRWKRGALEAAQLAAIEDAAI
                     LEAVRRQESIGLEAVTDGEFRRDWWHLDFLSQLDGVTTRDNPGPKFGGTEEQPPIATV
                     SGRVGCSKPIMVQHFAFLKSVARATPKFTLPSPSMLHLRGGRNAISTEAYPGLDAFWD
                     DVAAAYREAIAHLAAAGCTYLQLDDTAFAYLCDPKIRETCRRNGDDPALLPQRYADTI
                     NAALADKPAGMTVTLHTCRGNFKSAWVAEGGYDPVAKAMFSANVDGFFMEFDSARAGT
                     FEPLKALPKGKKVVLGLVTTKVGEIEDKAMLKRRIAEAAKFVPLENLCLSPQCGFSST
                     HHGNVLSEDDQWRKLELIVEVAREVWPGA"
     gene            8809..9483
                     /locus_tag="AC16O9_06600"
     CDS             8809..9483
                     /locus_tag="AC16O9_06600"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6516321.1"
                     /translation="MRVYYHPASTTSRIIMMFAAEEAPEIEFKTVDIFTGEHMKPPYA
                     DLNPSRQVPMLEDGDFRLTESSAILKYLADEKGSTSYPKELRARARVNEAMDWFNTQF
                     YRDFAYGLVYPQGFQTHKRRSDEAQAATLAWGKERAQAWLKVLDQHLIGPRNGYVCGS
                     KLTIADYLGGEMVAVGELIGCGFDGYPNVQRWLKNMKSLKSWPKVHEVFYGFAGSMKD
                     AKFDAI"
     gene            9497..9985
                     /locus_tag="AC16O9_06605"
     CDS             9497..9985
                     /locus_tag="AC16O9_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6516322.1"
                     /translation="MIRGLHHNAYRCRDSEQTRRFYEDFLGLPLAGTLWIKETMSGRK
                     TQTLHTFYRLDDGSYLAFFDAPDMPFEFKAQHDFDLHIALEVERAMLEPMMAKGKAAG
                     IETRGISDHGFIHSIYFRDPNGYVIELTARMPGHAQAMDPAQNEARAKLDRWQAARRQ
                     PA"
     gene            complement(9995..10852)
                     /locus_tag="AC16O9_06610"
     CDS             complement(9995..10852)
                     /locus_tag="AC16O9_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HIR6516323.1"
                     /translation="MPIRNFTEKNLTAEVLKRVGKAPDRRTRQIMQSLVKHLHAFVRE
                     VRPTQEEWFQGIQFLTKTGHMCDDKRQEFILMSDTLGVSMLVDFINYGKLGKATESTV
                     LGPFFVEGAPEMPLGASIAKPGTPGEPCAVSGTVKDLKGKPIAGATLDVWEGGADGLY
                     DVQKPDGTNLRARFRSDAEGRYHFKCITPVSYPVPHDGPVGKMLVACGRHPMRPGHLH
                     FVIEAPGHEKLVTHLFTKGDKYLDSDAVFGVKDSLIVDFRKKNAAGEATCRYDFVLKP
                     AGAGKSRRR"
     gene            10907..11854
                     /locus_tag="AC16O9_06615"
     CDS             10907..11854
                     /locus_tag="AC16O9_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6516324.1"
                     /translation="MKTLICALAAALVSGGALAQQGTIKFLVGFPAGASLDAMTRMVA
                     DKMQASLGQNVIVENRTGAAGQIAMGALKTSPADGTVLVMTPLVTIVTAPHIQKLPYD
                     PFNDFVPVAHAANFLFAFAMGPATPAKSLKEYIGLVKQDAKYGNYASAATGSLPHFFS
                     LLFAEKAGLKLNHIGYRGTAAAMTDLLGGQIAAFMGTVSDVAPQHNAGKITAVATSGA
                     TRSKHLPGVPTFRELGYDIEGGGWYAAYAPAGTPAPVVDRLSKAIIAALREPDVRERL
                     DNYGMEPTGLPPAELARIHRRDYEQWGPVIKASGFKPGS"
     gene            11851..12768
                     /locus_tag="AC16O9_06620"
     CDS             11851..12768
                     /locus_tag="AC16O9_06620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6516325.1"
                     /translation="MNLLFASTKEDPERWRKLLEPALPQDRFFVWPDVNGGAIDVALV
                     ATHPKGTFARLPRLKLIQSLWMGVENILADAEVPRDVPLARLIDPGMVHAMAETVIAS
                     VLDWHRHRYLYRAQQRERAWKWQKQFLPSDRAIGLLGLGELGSYAAAKLVALGFEVRG
                     WSRRPKALEGVHCSTDLDEVISRSQALVCLLPLTAQTRGVLNARAFARMPKGGCVINL
                     ARGGHVVTADLLAALASGRLAHAYLDVFEAEPLPADSPLWAHPGITITPHVAALTEPR
                     TALAKIVENVERVRRGETPLSLVDRAAGY"
     gene            complement(12884..13477)
                     /locus_tag="AC16O9_06625"
     CDS             complement(12884..13477)
                     /locus_tag="AC16O9_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR6516326.1"
                     /translation="MKSAGRIFLTGMLTVLPVLATVYLIVWLVTAAERFVGKQLMWLI
                     PDEYYRTGMGLVLALAAIFLVGLLMHALLFRQLFGWAERLMLEIPLVRSVYGALKDLF
                     GLFAGHEEDQAMQVVSVALPGTGMRALGFVSRADFSDLPEGIGRAGEVAVYLPMSYQI
                     GGYTLFIPREQAVPVAMSREEAMKFILTAGLKSGRGA"
     gene            complement(13514..14218)
                     /locus_tag="AC16O9_06630"
     CDS             complement(13514..14218)
                     /locus_tag="AC16O9_06630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516327.1"
                     /translation="MEKTNPTGKTVVVTGAARGIGKATADLFAAQGYSVVRLDIDPLE
                     GELRVDVSDEEAVAAAFEKIGRIDVLVNNAGVAVRKSALEISRAEWDRVLDVNVDGLF
                     FCSQAAARRMKASGGGAIVNLASIMGFSGGLFPNPAYQTSKGAVVNLTRALALEFAPL
                     GITVNAVAPTFVRTPLTAPVFSDPERLKLVMDHTPLGRLPEPEDIAQAILFLAGPGAR
                     CITGVTLPVDSGYLAR"
     gene            14239..>14728
                     /locus_tag="AC16O9_06635"
     CDS             14239..>14728
                     /locus_tag="AC16O9_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6516328.1"
                     /translation="MHDLVIRNALILDGLGTEPFVGELAVKDGKIAAIGTSLSGAESL
                     DAGGLALMPGIIDNHTHYDAQITWDPMVSPSPALGVTTAVIGNCGFTIAPCKPADREL
                     VMKNLTQVEGMSLEVLRQGIRWEFETIPQYFDMLERQGSAINVAGFVGHSSVRTYVMG
                     EDA"
BASE COUNT         2329 a         5177 c         4964 g         2258 t
ORIGIN      
        1 gggccgacgt cgtcgtggag gaaacctacg gcttcgggcg gcacaccggc gtgtgcaacg
       61 aagggcgcgc gatcctcgcc gactacaacg ccggcgagca ttcgctcacc gtgtaccacg
      121 cgacgcaggc gccgcacatg atgcaggaca tcttctcgcg ccacctcaat atccccgagg
      181 cgaacgtgcg cgtgatcacc aaggacgtgg gcggttccta cggcatcaag gtgcacgtgt
      241 accccgacga gatggcgacc gcggcgctct cggtgatgct gcgacgcccg gtgaagttcg
      301 tcgccgaccg gctggaatcg ttcctgtccg acatccacgc gcgcgagcac aaggcgaagg
      361 tgcgcctcgc ctgcaccaag gcgggcgaga tcctcgcctt cgagctcgac gacctcacgc
      421 cgatcggccc gtactcggtg tacccgagga cgagcggcat cgagggcaac caggtggtga
      481 acctcaccgg cggcccgtac aagcaccaga agtaccgcgc gaagatgcac gtcgtcttca
      541 ccaacaagaa cgtcacctgc cagtaccgcg cggtgggcca cccgatcgcg gtggcgctca
      601 ccgaagcgat cgtcgacctc gcggccgcga agctcggcat ggacccggcc gagttccgcc
      661 gcaagaacct gatccccgac gacgcctacc cgtacacctt cccgtccggc atcaagttcg
      721 agaagctctc gcaccaccag acgctcgaca agctgctcgc gctcatggac tacaagaagc
      781 tgcgcgagga gcaggcggcg ctgaggaaaa agaaggtcta ccgcggcatc ggcctcgcgg
      841 cgatgatcga ggtcaccaac ccctcgcccg ccttctatgg cgtgggcggc gcgcgcatct
      901 cggcgcagga cggcgccacg ctgcggctcg agcccaccgg catggtgacg gtgatgtgca
      961 gcgtcaccga gcagggccag gggaccgaag gcatcttcgc gcagatcgcg gcgagcgcgg
     1021 tcggcgtcac gatggacaag gtgcgcgtga tcaccggcga caccggcgtc acgccctacg
     1081 gcgggggcac ctgggcctcg cgcggcgcgg gcatcggcgg cgaggcggtg ctgctcgccg
     1141 ggcgggccct gcgcgccaac atgatcgagg ttgcggcggc gatcctgaag gtggacaagg
     1201 ccgagctcga cgtgcaggcg aacgccgtgg tgcacaagct cacgcaggag cagaagatgc
     1261 cgctcgccga gctcgggcgc gtcggctact tccgtcccga cacgctgccg cccgatttcc
     1321 aggccgagct cacggtgacg cgccactaca cgccgcgcca gtacggcttc accttcacca
     1381 acggcattca ggcctcgctc gtcgaggtcg atcccgacac ggggctggtg aagctgctca
     1441 agcactggtg cgtcgaggac tgcggcacgg tgatcaaccc gatgctggtc gacgagcaga
     1501 tccgcggcgg cgtggtgcag ggcatcggcg cggcgctgta cgaagagtgc ctctacgacg
     1561 cgagcggcca gctcctgaac ggcaacatgg ccgactacct cgtgccgatg gcgctcgaga
     1621 tgcccgacat cgtctgcgcg cacatcgaga cgccgacgaa gcagtcgcag ctcggcgcca
     1681 agggcgtggg cgaagccggc accgcgggcg cgccgggcgc ggtgatgaac gccatcaacg
     1741 acgcgctcgc cccgctcaac gcgcgcgtca ccagcatgcc gttcacgccc gagcgcatcc
     1801 tgcgcgcgct cggcaaggtg gcttgaagct ctacggctac tggcgctcgc tcgccacgtt
     1861 ccgcgtgcgc gtcgcgctcg ctctcaaggg gttgcggctc gatcagcccg agatcccgat
     1921 cgacctgatg caggggatgc agcacgacgc ggcctaccgc agggtcaacc cgcaggcggt
     1981 ggtgccggcg ctgatcctcg aggacggtgg cgcgccgctc tttcagtcga tggcgatcct
     2041 cgagtacctc gaggagacgc acccgctgcc ggccctgctg cccaaggatg cacgcggccg
     2101 cgcgcgggtg cgcggcctgg cgcagatctg cgtgagcgac ggccatccct tcatcgtgcc
     2161 gcgcgtgcgc ggctacctgg agaagacgct cggtctggac gaggcggcgc gcaaccgctg
     2221 ggtcgcgcac tggtcgctgc aggcgctcga ggcgctggag ggccacctcg cgcgcgagcc
     2281 cgggaccgcg cgctactgcc acggcgacgc gcccacgctc gccgacgtct gcctcgcgtc
     2341 gcaggtggtc gcggcgaagg actatggccg ctgcgacgtt tccggcgtcc ccacggtgat
     2401 gcgcatctac ggcgagtgca tgaagcaggg ggcgttcgcg caatcgcatc cggcgcgcca
     2461 gcccggcgcg ccggccgacc tgcggccctg atgggagtgt gcgcgttgac gctcgcgctg
     2521 gggtcgtggc acgatgggcc cggggagtct caccaaggag gaggaaaccc atgaagagaa
     2581 tctgtgtcgc gctgctcgcg ttcgcctttg cggccctgtc cggggcggcg gcggcagccg
     2641 agcccatcaa gatcggcttt tcgatggcgc tcaccggcgg ccttgccggc gccggcaagg
     2701 cggcgctcat cgccatggag atctggcgcg aggacgtgaa caagaagggc ggcctgctcg
     2761 gccggcaggt ggagttcgtc tactacgacg acgccaccca gcccgccaag gtgccgccga
     2821 tctacaccaa gctgctcaac gtcgacaagg tcgacctcgt ggtgtcctcc tacggcacca
     2881 acgagatcgt gccggcgatg ccgatcgtga tgcgcaacaa gatggtgttc ccgtcgctgt
     2941 tcgggctcgc cgcgaacgag gagttcaagt acaaccgcta cttccagatc atgcccgcgg
     3001 ggcccgaccc caaggtggac tggtcgcgcg gcttcttcga gctggcggcg cagcagaacc
     3061 cgaagcccaa gaccgtcgcg atcgtggccg cggacgcgga gttcccgatc aacgccgcca
     3121 ccggggcgcg caagaacgcg caagccgcag gcctgaagat cgtctatgac aagacctacc
     3181 cgccggcgac gaccgactac acgccgatcg tgcgcgccat ccaggcgacc aacgccgaca
     3241 tcgtgtacgt cgcctcctat ccgcccgatt ccgtcggcat ggtgaaggcg gccaacgagg
     3301 tcggcctgaa ggcgaagatg ttcggcggcg gcatggtcgg gctgcagttc gcggccatcc
     3361 agaagaacct ggggccgctg ctgaacggcg tcatcaacta cgacttctgg gtgccggagc
     3421 cgacgctcga ctttccgggg gtgaaggagt ttctcgcgcg ctaccaggag gcggccaagg
     3481 acaagggcgt cgacccgctc ggccactacc tgccgccctt cgcctacgcc tacatgcaga
     3541 tcctgggcaa cgcggtgcag gcgaccaaga gcctggacca gggcaagatc gccgactact
     3601 tccgcaagaa cacgcacgac acggtggtcg gcaaggtgaa gttcggcaag aacggcgagt
     3661 gggccacctc gcgcgtgctg atggtgcagt tccgcgacgt gcagcccgac aacctggagc
     3721 agttcgccgg tccgggcaag cgcgtggtgc tgtacccgaa ggcgtggaag tcgggagaca
     3781 tcgtcttccc gttcaagcag tagccgcgcg ccggtcctga cgcgcccggg cgacggtgtt
     3841 ctcggctgat cttttcgcga acgccgtcgt ctcggggctg ctgctcggcg gcttctacgc
     3901 cgccgtggcc atcgggctgg cgatcgtctt cggccagctc gacatcgtca acatcgcgca
     3961 cccggctttc atcatcgccg gggcctacac cgcctacatc ttcaacacca ccttcggctt
     4021 cgacccgatc ctggtcgggg tggcggcgat gccggtgttc ttcgccgtgg gggtggtgat
     4081 ctatcgcatc tactacgccg cgttcgagcg caccgggcag gagtcgctct cgggcctcgc
     4141 cttcttcttc ggcctcatgt tcatcatcga ggtgtcgctg atcctcgtct acggcgtcga
     4201 ctaccgcctg gtgagcgccg gctactcggc gcagacgctc gccttcggct tcgtcgacct
     4261 gccggggcgc atgctgatcc cgttcctcgt cgcgatgacg ctgaccggcg ccgtgtggct
     4321 gtacctgtcg cgcaccttca tgggccgcgc catccaggcg gtggcgcagg accgcggcgc
     4381 gctgcagctg atgggggccg acccggtgcg catcaagcag tgggccttcg gcatcggcat
     4441 cgccaccgcg gcggtggcgg gggcgctgct gatcatcatc ggcccggtcg agccctcggt
     4501 cgggcgccag tacatcgggc gcgcgttcgc catcgtcgtg ctgggcggca tgggcagcat
     4561 ccagggcatg ctgatcgccg cgctggcgct cggggtggtc gagaacatgg tgtcgacctt
     4621 ctatggcccg tcgtgggcgc ccgccgtcgc cttcggcgtg ctgctcgcca cgctcgcctt
     4681 ccggccctcg ggcctgttcg ggaggtagcg gtgcgcggct ggggcttctg ggccgcgagc
     4741 gccgcggcgc tggccgcggg cctggcgttc accgcgctcg ccgacaaccc gtactacttc
     4801 aacgccacct acgtgatcct gcaggcgctg atcatggcgg tggcctggaa catcctgggc
     4861 ggcttcaccg gctacgtgaa cttcggcagc gccgccttct tcgccgtcgg cgcctacatc
     4921 tcgatcgccc tgctgaagac cgcggacgtg ccgctgccgc tgctgatcct cgcctcggcg
     4981 ctggtgtgcg gcctgatcgg cctgggcgtg ggctacctga cgctgaggct gaagggcgtg
     5041 tacttcgcca tcgccacgct cgccatgggc atcgtgctgg agacgctggt caccaactgg
     5101 agctacgtgg gcggcgccaa gggcacggcc atcctcaccc cggagacgca cctgtggttc
     5161 gacagctaca gcgagatgct gttcatggtc atgctgctgc tcgcgctcgg ctcggtcgcg
     5221 atatcgcgcg ccctctcgag ctcgcgcatc ggccgcgggc tcgccgcgat ccgcgacgac
     5281 gaagtggcgg ccgagtgctc gggcgtgccc acgctgcgcc tgaagctggt ctcgaccacg
     5341 gtgatgggcg cgatgatggg cgcggccggc gcgccctacc cgtacttcgt cacctacgtc
     5401 gacccgatct ccgccttcaa cctgttcatc gccgtcaacg ccatcgccat gccgatgatc
     5461 ggcggcaccg cccactgggc cgggccggtg atcggcgcgc tgctcgtagg ctcgatgcag
     5521 gaagtgatca cggtcaccat ctcctccgag ctcaacgtgc tgctggtcgg gatcatgctg
     5581 gtgctgttca tcacgctggc gccgcagggc atcgtcggcc tcgtgcgcaa gcactggaag
     5641 cgccggaagt ccgcatgagc gccctgctcc aggtcagcgg cctcgccaag cgcttcggcg
     5701 gcttcaccgc gctcgagggc attacgctcg cggtcgcccc cggcgagcgc ctcggcctca
     5761 tcggccccaa cggctcgggc aagaccaccc tgatcaactg cgtctcgggg gcgctgttca
     5821 acgacgaggg ccgcgtcgag ttcgaaggcc gcgacgtcac gcgcctgccc ccgttccagc
     5881 gcgcgcgcct cggcatcgcg cgcagcttcc agatccccaa gccctttcgc agcatgacgg
     5941 tgctcgacaa cctgcgcgtg ccgctcgagt acgcggcgcg cacgcgcgcc gggcagcgca
     6001 gcgcggtaga cgaggcgatg cgcatcctgg agaccatggg cctcgccgcg cgcgcgcgcc
     6061 aggactcggc cggcctcacc caggtggaca tgaggaagct cgagctcgcg cgcgccatgg
     6121 ccgcgcaccc gcgcctgctg atctccgacg aggcgatggc ggggctctcg ggggccgagg
     6181 tggacgaggt gctggagatc ctgttcaagc tgaacggtgc gggcatcacc atcatcatga
     6241 tcgagcacat catgcaggcg gtgatgcgct tctcgcagcg cgtggtgtgc ctggacgccg
     6301 ggcgcatcat ctgcgagggc acgcccgacc agacggtcgc gaacccggac gtgcagaggg
     6361 cttaccttgg cgcttagcct gaccgtgcgc ggggtgcaag ccggctacgg cgcggtgcgc
     6421 gcgctgcacg gcgtctcgct cgacatccag gacaccgaga cggtcgcgct cctcggcacc
     6481 aacggcaacg gcaagagcac gctcatgaag tgcgtgatgg gcatcgtgcg cccgacgcag
     6541 ggctcgatcg tgctgcaggt ggacggcgcc gagcacgacc tgacgcgcct caccaccgag
     6601 cagatcgtga acctcggcgt cgccatggtg cccgagggcc ggcgcctgtt cccgaagctc
     6661 accgtggagg agaacctgct gctcggcgcg ttccgccccg ccgcgaggag cggcatcgcc
     6721 aagaacctcg ccttcaccta cgaagcattc ccggtgctcg ccgagcgccg gcgccagctc
     6781 gccggctcca tgtcgggcgg ccagcagcag atgctcgcca tcggacgggc gctgatgtcc
     6841 gcgccgcggc tactgctggt cgacgagcct tcggtgggcc tcgcgccgat tctggtgtcc
     6901 accaccatca ccaagatcaa ggagctgaag gaccgctacg gcctcaccgt gctgatggcc
     6961 gagcagaact tccaccaggc gatccgtatc gccgaccgcg gctacatcat cgtgcacggc
     7021 gagatcgtgt tcgacgggcg ctcggtcgcc gagctcgagg gaaacgatct ggtgaagcgc
     7081 tactacctcg ggacctgacc gcggcgcccc acgcctcgca gcggcgcatc gccgcgggcg
     7141 tgaactcggc gcgcggctgg ttgccgccga tgacgaacat cagcaggtgc tccttcttcg
     7201 gctcggcgta ggtcacgttc atgaagcgcc gcgcgacgcc cggcgggaac gagatcacgt
     7261 cgtagggccc gaggtcggcg tgcagcttct tgcggccctc gttccactcg cagcgccagc
     7321 gcccggtcat cgggatgaag gtctcgttgg tgtcgtggtt gtgcatcagc ggccccttgc
     7381 cgggcttcgc cttgcagtag ccgaggttga agccttcggc gatggcgatc gcgggcatcg
     7441 cggaggcgtc gtcgcccacg ggcgaagtcg tcgccctggt ccccttcttc ggcggcgcga
     7501 agccgatcac ggcgtagagc gtgcgctcgc actcgggcaa ggggctgtcg ggcagcccgc
     7561 cgtcggagcc cttcagccgc gaaaagcgcg ccacgcgctt cagcatcgcg cgcttggaca
     7621 gggtacgaat ccgcttccag tcagccatcg tcgcctcctc gagttagcct atcatgcccc
     7681 tcatgaagcc gccgttccgc gccgaccagg tcggcagcct gctgcgcccg gcggcgctcg
     7741 ccgaggcgcg gcgccgatgg aagcggggcg cgctcgaggc cgcgcagctc gccgcgatcg
     7801 aggacgcggc cattctcgag gcggtgcgtc ggcaggaatc gatcggcctc gaggcggtga
     7861 ccgacggcga gttccgccgc gactggtggc acctggattt cctgtcgcag ctggacggag
     7921 tgaccacgcg cgacaatccc gggcccaagt tcggcggcac cgaggagcag ccgccgatcg
     7981 ccaccgtcag cggccgggtc ggctgctcga agccgatcat ggtgcagcac ttcgcgttcc
     8041 tgaagtccgt ggcccgggcg acgccgaagt tcaccctgcc ctcgccttcc atgctgcacc
     8101 tgagaggcgg ccgcaacgcc atctcgaccg aagcctatcc cgggctcgac gcgttctggg
     8161 acgacgtcgc cgcggcctac cgcgaggcga tcgcgcacct cgccgccgcg ggctgcacct
     8221 acctgcagct cgacgacacg gcgttcgcct acctgtgcga cccgaagatc cgcgagacct
     8281 gccgccggaa cggcgacgac ccggcgctcc tgccgcagcg ctatgccgat acgatcaacg
     8341 ccgcgctggc cgacaagccg gcgggcatga ccgtcaccct gcacacctgc cgcggcaact
     8401 tcaagagcgc gtgggtggcc gaaggcggct acgacccggt ggccaaagcc atgttctcgg
     8461 ccaacgtcga cggcttcttc atggagttcg attcggcgcg cgccggcacc ttcgagccgc
     8521 tgaaggcgct gcccaagggc aagaaggtgg tgctcggcct ggtcaccacc aaggtcggcg
     8581 agatcgagga caaggcgatg ctgaagcgcc gcatcgcgga agccgcgaag ttcgtgccgc
     8641 tggagaacct ttgcctgtcg ccgcagtgcg gcttttccag cacgcaccat ggcaacgtgc
     8701 tgtcggaaga cgaccagtgg cgcaagctcg agctgatcgt cgaggttgcg cgcgaagtgt
     8761 ggccgggagc atagaatcga gcctggctta ccgaggaggg ccgagaccat gcgcgtttac
     8821 taccatccgg cgtcgaccac cagccggatc atcatgatgt tcgcggcgga agaagcgccg
     8881 gagatcgaat tcaagaccgt cgacatcttc accggcgagc acatgaagcc gccctacgcc
     8941 gacctgaatc ccagccgcca ggtgccaatg ctcgaggacg gcgacttccg gctgaccgag
     9001 agctcggcga tcctcaagta cctggccgac gagaaaggct ccacgtccta cccgaaggag
     9061 ctgcgcgcgc gcgcgcgggt gaacgaggcg atggactggt tcaacacgca gttttatcgc
     9121 gacttcgcct acggcctcgt ttacccgcag ggcttccaga cgcacaagcg ccgcagcgac
     9181 gaggcgcagg ccgcgacgct cgcctggggc aaggagcggg cgcaggcctg gctcaaggtg
     9241 ctcgaccagc acctgatcgg gccccggaac ggctacgtct gcgggtcgaa gctcaccatc
     9301 gccgactacc tcggcggcga gatggtggcg gtgggcgagc tcatcggctg cggcttcgac
     9361 ggctacccga acgtgcaacg ctggctgaag aacatgaagt cgctcaagag ctggcccaag
     9421 gtgcacgagg tcttctacgg cttcgccggc tccatgaagg acgcgaagtt cgacgccatt
     9481 tgaggggcat cggcccatga tcaggggact gcaccacaac gcctaccgct gccgcgactc
     9541 cgagcagacg cgccgcttct acgaggactt cctcggcctg ccgctcgccg gcacgctgtg
     9601 gatcaaggag acgatgagcg gccgcaagac gcagacgctg cacacgttct accgcctcga
     9661 cgatggctcg tacctcgcct tcttcgatgc gcccgacatg ccgttcgagt tcaaggcgca
     9721 gcacgatttc gacctgcaca tcgcgctcga ggtcgagcgc gcgatgctcg agccgatgat
     9781 ggcgaagggc aaggccgcgg gcatcgagac gcgcggcatc tcggatcacg gcttcatcca
     9841 ctcgatctac tttcgcgacc cgaacggcta cgtcatcgag ctcaccgccc gcatgccggg
     9901 ccacgcgcag gcgatggacc cggcgcagaa cgaggcgcgc gccaaactcg atcgctggca
     9961 ggcggcgcgg cggcagccag cctagggacg agctttatcg cctgcgcgac ttcccggcgc
    10021 cggcgggctt gagcacgaag tcgtagcggc aggtcgcctc gccggcggcg ttcttcttcc
    10081 ggaagtccac gatcagcgag tccttcacgc cgaacacggc gtcggagtcg aggtacttgt
    10141 cgcccttcgt gaacaggtgc gtgacgagct tctcgtggcc cggcgcctcg atcacgaagt
    10201 gcaggtgccc ggggcgcatc gggtggcggc cgcaggcgac cagcatcttg cccacggggc
    10261 cgtcgtgcgg caccgggtag ctcaccggcg tgatgcactt gaagtggtag cgcccctcgg
    10321 cgtccgagcg gaagcgcgcg cgcaggttgg tgccgtcggg cttttgcacg tcgtacaggc
    10381 cgtcggcgcc gccctcccag acgtcgagcg tcgcgcccgc gatcggcttg cccttgaggt
    10441 ccttcaccgt gccgctcacg gcgcagggct cgccgggcgt gccgggcttc gcgatgcttg
    10501 cgccgagcgg catctcgggc gcgccctcga cgaagaacgg cccgagcacg gtggactcgg
    10561 tcgccttccc cagcttgccg tagttgatga agtccaccag catcgacacg ccgagcgtgt
    10621 ccgacatcag gatgaactcc tggcgcttgt cgtcgcacat gtgcccggtc ttggtgagga
    10681 actggatgcc ctggaaccac tcctcctgcg tcgggcgcac ctcgcgcacg aaggcgtgca
    10741 ggtgcttgac caggctctgc atgatctggc gcgttctcct gtcgggcgcc ttgccgacgc
    10801 gcttcaggac ttcggcggtg aggttcttct cggtgaaatt gcggatgggc atggccggct
    10861 cctcctcgcg cgcaatgcta acatcgcgca cagaggagga atcaccatga agacactgat
    10921 ctgcgcgctt gccgccgcgc tcgtctccgg cggcgccctc gcccagcagg gcaccatcaa
    10981 gttcctggtc ggctttcccg ccggcgccag cctggacgcc atgacgcgca tggtggccga
    11041 caagatgcag gcctcgctcg gccagaacgt gatcgtcgag aaccgcaccg gcgccgccgg
    11101 ccagatcgcc atgggcgcgc tgaagacctc gcccgcggat ggcacggtgc tggtgatgac
    11161 gccgctcgtc accatcgtca ccgcgccgca catccagaag ctgccctacg atccgttcaa
    11221 cgatttcgtg ccggtggcgc acgccgcgaa cttcctgttc gccttcgcca tgggcccggc
    11281 gacgccggcg aagtcgctca aggaatacat cggcctggtg aagcaggacg cgaagtacgg
    11341 caactacgcc tccgcggcca ccggcagcct gccgcacttc ttctcgctgc tgtttgccga
    11401 gaaggcgggc ctgaagctca accacatcgg ctaccgcggc accgccgcgg cgatgaccga
    11461 cctgctcggc gggcagatcg cggccttcat gggcaccgtg tccgacgtcg cgccgcagca
    11521 caacgcgggc aagatcacgg cagtcgccac ctccggcgcg acgcgctcga agcacctgcc
    11581 cggcgtgccg accttccgcg agctcggcta cgacatcgag ggcggcggct ggtacgcggc
    11641 ctacgccccc gccggcacgc cggcgccggt ggtcgatcgc ctctccaagg caatcatcgc
    11701 cgcgctgcgc gagcccgacg tgcgcgagcg gctcgacaac tacggcatgg agcccacggg
    11761 cctgccgccc gccgagctcg cgcgcatcca ccgccgcgac tacgagcagt ggggcccggt
    11821 gatcaaggcc tccggcttca agcccgggag ttgaacctcc tcttcgcctc cacgaaggag
    11881 gatcccgagc gctggcgcaa gctgctcgag ccggcgctgc cgcaggatcg cttcttcgtc
    11941 tggccggacg tgaacggcgg cgcgatcgac gtcgcgctgg tcgccacgca tccgaagggc
    12001 accttcgcgc ggctgccgcg gctgaagctc atccagtcgc tgtggatggg cgtggagaac
    12061 atcctcgccg acgccgaagt cccgcgcgac gtgccgctcg cgcgcctcat cgaccccggc
    12121 atggtgcacg ccatggccga gacggtgatc gcgagcgtgc tcgactggca ccgccatcgc
    12181 tacctgtacc gcgcccagca gcgcgagcgc gcctggaagt ggcagaagca gttcctgccg
    12241 tccgaccgcg ccatcgggct gctcggcctg ggcgagctcg gcagctacgc cgccgcgaag
    12301 ctcgtcgcgc tgggcttcga ggtgcgcggc tggagccgcc ggccgaaggc gctcgagggc
    12361 gtgcactgct cgaccgatct cgacgaggtg atctcgcgct cgcaggcgct ggtctgcctg
    12421 ctgccgctca ccgcgcagac ccgcggcgtg ctgaacgcgc gcgcgttcgc gcgcatgcca
    12481 aagggcggct gcgtcatcaa cctcgcgcgc ggcggccacg tggtgaccgc ggacctgctc
    12541 gccgcgctcg catccgggcg gctcgcgcac gcctacctcg acgtcttcga ggccgagccg
    12601 ctgcccgcgg actcgccgct gtgggcgcat cccggcatca ccatcacgcc gcacgtcgcg
    12661 gcactcaccg agccgcgcac ggcgctggcg aagatcgtgg agaacgtcga gcgcgtgcgg
    12721 cgcggcgaga cgccgctcag cctcgtcgac cgcgcggcgg gctactgacg cgctccggcg
    12781 ccggcggcgc gccgacgtag gcgtgtccgc cacggcgctc ccgcgccttg cgcaggcggg
    12841 cggcgagcgc cccgttcatg gccgcgctct cgtcaaatgc aggtcaagcc ccccgccccg
    12901 acttcagccc ggcagtaagg atgaacttca tcgcctcctc gcgcgacatg gcgaccggca
    12961 ccgcctgctc gcgcgggatg aacagcgtgt agccgccgat ctggtagctc atcggcaggt
    13021 acaccgcgac ctcgcctgcg cggccgatgc cctcgggcaa atcggagaaa tcggcgcgcg
    13081 agacgaagcc gagcgcgcgc atgccggtgc ccggcagcgc caccgacacc acctgcatcg
    13141 cctggtcttc ctcgtgcccg gcgaacagcc cgaacaggtc cttcagcgcc ccgtacaccg
    13201 agcgcaccag cgggatctcg agcatcagcc gctcggccca gccgaaaagc tggcggaaga
    13261 gcaacgcgtg catcagtagc cccaccagga agatcgccgc cagggcgagc accaggccca
    13321 tgcccgtgcg gtagtactcg tcggggatca gccacatgag ctgcttgccg acgaagcgct
    13381 cggccgccgt gaccagccag acgatgaggt aaacagtggc cagtaccggc agcacggtca
    13441 gcatgccggt aaggaagatc ctgcctgcgg atttcatgcg cgacctcgtt ctcgggtggg
    13501 ccagcggccc accctagcgc gcgaggtagc ccgagtctac cggcagcgtc accccggtga
    13561 tgcagcgcgc gcccggcccg gcgaggaaca ggatcgcctg cgcgatgtcc tcgggctccg
    13621 gcaggcggcc gagcggggtg tggtccatga ccagcttcag ccgctccgga tccgagaaca
    13681 ccggcgcggt gagcggcgtg cgcacgaagg tcggcgccac ggcgttcacc gtgatgccga
    13741 gcggcgcgaa ctcgagcgcg agggcgcgcg tgaggttcac caccgcgccc ttggaggtct
    13801 ggtaggcggg attggggaac aggccgccgg agaaccccat gatcgaggcg aggttgacga
    13861 tggcgccgcc gccgctcgcc ttcatgcggc gcgccgccgc ctgcgagcag aagaaaaggc
    13921 cgtccacgtt cacgtcgagc acccggtccc actccgcgcg cgagatctcg agcgcgctct
    13981 tgcgcacggc gacgccggcg ttgttgacca ggacgtcgat gcgcccgatc ttctcgaacg
    14041 ccgccgccac ggcctcttcg tcgctgacat cgacgcgcag ctcgccctcg agcgggtcga
    14101 tgtcgagccg gaccaccgag tacccctggg cggcgaacag gtccgccgtg gccttgccga
    14161 tgccgcgcgc ggccccggtc acgacgaccg ttttgcctgt cgggtttgtt ttttccatgg
    14221 tgccgctagt ataggggcat gcacgacctt gtcattcgaa acgccctgat cctcgatggg
    14281 ctcggaaccg agccgttcgt tggcgagctc gccgtcaagg acggcaagat cgccgccatc
    14341 ggcacgtcgc tgtccggcgc ggaatcgctc gacgccggcg gcctcgcgct gatgcccggc
    14401 atcatcgaca accacacgca ctacgacgcg cagatcacct gggacccgat ggtctcgcct
    14461 tcgcctgcgc tcggcgtgac cacggcagtg atcggcaact gcggcttcac catcgcgccg
    14521 tgcaagcccg ccgatcgcga gctggtgatg aagaacctca cgcaggtcga gggcatgtcg
    14581 ctcgaggtgc tgcgccaggg gatccgctgg gagttcgaga ccatcccgca gtatttcgac
    14641 atgctcgagc gccagggcag cgcgatcaac gtcgccggct tcgtcgggca ctcgtcggtg
    14701 cgcacttacg tgatgggcga agacgcgc
//
LOCUS       DBNHKE010000107        11756 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_336019, whole genome shotgun sequence.
ACCESSION   DBNHKE010000107 DBNHKE010000000
VERSION     DBNHKE010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_336019"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="AC16O9_06640"
     CDS             complement(<1..125)
                     /locus_tag="AC16O9_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6516329.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTHILAKKYGG"
     gene            complement(151..2247)
                     /gene="fusA"
                     /locus_tag="AC16O9_06645"
     CDS             complement(151..2247)
                     /gene="fusA"
                     /locus_tag="AC16O9_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6516330.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVSHKMGEVH
                     DGAAVMDWMEQEQERGITITSAATTCFWKGMDQSYPEHHINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYDAVAGVQPQSETVWRQANKYRVPRLAFINKMDRVGANFFKSYDHIRN
                     RLKGNPVPIQIPIGAEEKFAGVVDLVTMDAIYWDDATQGMKFERKPIPADLTAQAKEW
                     REKMVEAAAEANEVLMNKYLESGELSTEEVKRGLRLRTINNEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDIPPVKGENDKGEPDVRKPGDDEPFAALAFKIMTDPFVGQLSFI
                     RVYSGVLTSGDTVYTSVRGKKERIGRLLQMHANERKEIKEVRAGDIAAVVGLKDVVTG
                     ETICDVNKVITLERMEFPDPVISQAVEPKTKVDQEKMGVALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEASVGKPQVAYRETFKKSLDSEGKFIKQSGG
                     RGQYGHVWLKLEPQPVGKGYEFVDAIKGGRVPREFIPAVKKGVDEALQDGVLAGYPVV
                     DVKATLFDGSYHEVDSNENAFKMAAIFAFKDGMRAATPVLLEPIMAVEVETPEEKMGD
                     VMGDLSARRGVIQGMDDLAGSKTIKAEVPLGEMFGYSTTLRSLTQGRATYTMEFKHYA
                     EAPKSVAEAVITARSK"
     gene            complement(2267..2737)
                     /gene="rpsG"
                     /locus_tag="AC16O9_06650"
     CDS             complement(2267..2737)
                     /gene="rpsG"
                     /locus_tag="AC16O9_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR6516331.1"
                     /translation="MPRRREVPKREILPDPKFNHPEVAKFINVLMTRGKKSVAEGIVY
                     RAFDVIKTKSGKDPVEVFTQAVQNVKPVVEVKSRRVGGANYQVPVEVRPVRRVALAMR
                     WIREAAQKRGEKSMTVRLAGELQEAAEGRGGAMKKREEVHRMADANKAFAHYRF"
     gene            complement(2761..3138)
                     /gene="rpsL"
                     /locus_tag="AC16O9_06655"
     CDS             complement(2761..3138)
                     /gene="rpsL"
                     /locus_tag="AC16O9_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR6516332.1"
                     /translation="MPTVNQLRRGIRAARRPKSKVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQSRSKYGAKAPKTA"
     gene            complement(3238..3735)
                     /locus_tag="AC16O9_06660"
     CDS             complement(3238..3735)
                     /locus_tag="AC16O9_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HIR6516333.1"
                     /translation="MDASVRRAMARWPDVPAVYGWLALDRRGNWLLQDSAGGGFGRIG
                     NAMLRDFIARNYAHDERGCWYFQNGPQRVFVALAYTPLVLRYEGETLVDHCGRPVRAR
                     ATFVDEEGSVLIEDERGVGLLDDRDLALFAARLGDNWRALPEVASHAVAARFGFVATP
                     SHSLR"
     gene            complement(3746..4585)
                     /locus_tag="AC16O9_06665"
     CDS             complement(3746..4585)
                     /locus_tag="AC16O9_06665"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6516334.1"
                     /translation="MTTSTAGSSAAANEEKFLEEMNRELAGWRDPAARLRQALARDEL
                     QLFCQPILSLRPPGAFAMAEALVRLREEEALLLPPGDFLPAFEHYRMMGELDRWVVRH
                     AVRWLAHPPAGGVPRLSLNVSGQALADAAFPRFVAEELLAQRVSPAALVFEVDEADTL
                     SRLEAAARFAHAVKQTGCKVLIDGFGQRSVSFIALKTMRVDLVKVDGSIVRALLRSEV
                     ARQKMKAVVRVGEAIGVEVIAECVEEPEIVAQLRRLGVGYAQGFGIARPAPIDRSAAP
                     PVP"
     gene            4669..5109
                     /locus_tag="AC16O9_06670"
     CDS             4669..5109
                     /locus_tag="AC16O9_06670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6516335.1"
                     /translation="MPREDFACAHRLRVRWAEVDMQRIVFNGHYLTYIDTAIAEYWRA
                     IGLPYPEGYVERYASDLFLRKATVEYLGSAGYDDELEVLCRASRLGRSSMTFLFEIWR
                     GASELLVTAELVYVNADPKAKTAAPLPDEVRERIRGFEKSGPAQ"
     gene            5106..5555
                     /locus_tag="AC16O9_06675"
     CDS             5106..5555
                     /locus_tag="AC16O9_06675"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6516336.1"
                     /translation="MTLYWEDFNVGDTVEMGRHTFTEAEIIAFARQFDPQPFHVDPQA
                     AMQSAFGGLIASGWHTCAVCMRLNCETYINRSVSLGSPGLDNIRWLKPVRPGDTISYR
                     RTVLESRASQSRPGVGLLRSRTEALNQAGELVMSMEGWGMFGRRPAP"
     gene            5552..6295
                     /locus_tag="AC16O9_06680"
     CDS             5552..6295
                     /locus_tag="AC16O9_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR6516337.1"
                     /translation="MSAKIELQHRIHVLTRKEELDGVRVAGKVVIVLDILFATTTMVA
                     VLAGGATEVLPVVDESAARDAGARHAEGGYVLAGELYAETLPGFAHPAPMALLAHGVA
                     GKSVIYATTNGTVAMTLAVGAKRVYCGSLLNAGALVSRVVAEHPQDRVLILCSGSGGN
                     FNLEDFYGAGCFVERFTRLLGPGADLSDAARTSLAFFRGARAPEALLECRVGRMMAAR
                     GLAHEVEFACRVDAFPIVPVLETGRLRCA"
     gene            6286..7050
                     /locus_tag="AC16O9_06685"
     CDS             6286..7050
                     /locus_tag="AC16O9_06685"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6516338.1"
                     /translation="MRVAALAVYPVKGAAAVPLERVALDNMGPLGDRRYCLARPSGRV
                     FTQRDDASLARLSAQPHDGGLLLEFDGERLQVGAADFATAAQLHVWSRQTAARIARNA
                     LNAALTRWFGTPLQLARLERPVERQGAMLAFGDAAALLLTNAASLDALNASMDVAVPM
                     ARFRPNIVLAGAAAFDEDRWQRLRVGSAILAPVHACGRCEVTTIDQEVGAVVDGEPLR
                     TLSRLRMRDGEPVFGVRYAVAQPGDIRVGDRAFDAP"
     gene            complement(7057..8190)
                     /gene="clsB"
                     /locus_tag="AC16O9_06690"
     CDS             complement(7057..8190)
                     /gene="clsB"
                     /locus_tag="AC16O9_06690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HIR6516339.1"
                     /translation="MRYVDGNRLTLLCNGEQYFPALVEAIDGARREVFLETYIFAGDE
                     TGSLVIDALARAAARGVAVRLLIDGFGSRGFPPRFRRILEDTGVQLHMYRPVRIWGLR
                     RDRLRRLHRKNACIDGQLAFVGGINILDDYVGPMPSPPRLDYAVRIEGPLTAQVRERA
                     ARLWARLAGEAPRAPVPARGPFAEGHRAALVVRDSLHHRRDIERVYLERIAAARDEVI
                     IANAYFLPGWRFRRALVAAARRGVRVVLFLQGRVEYLLLHLASRAMYGQLLDAGIEIW
                     EYQRSFLHAKVAVFDGVHCCVGSSNIDPYSLLMALEANVFVDDRRFSAQLRGGLQEAM
                     RNGAQRIPPQHWRRRPLWQRMPIWVAYSITRLLTAFAGYERYH"
     gene            complement(8195..8944)
                     /locus_tag="AC16O9_06695"
     CDS             complement(8195..8944)
                     /locus_tag="AC16O9_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6516340.1"
                     /translation="MSAVLRVVTLNMHKGLSQFNRRVVIHELREGLNTLEPDLVFLQE
                     VQGLNERHALRFATWPSAPQHEYLKQEGWQYAYGLNRVHHYGHYGNAVLSRFPIVSTE
                     NVDISSHRFERRGLLHCVLSVPGWRRELHCVCVHLSLHERGRRRQLEAIVERLGEAAA
                     RGEPIVVAGDFNDWRQRATRVLEERLGMQEVTAIYGDRPARTYPSLLPVLRLDRIYVR
                     GFSVVEASVHVGAPWSLLSDHLALGAVVKAA"
     gene            complement(8941..9390)
                     /gene="nudB"
                     /locus_tag="AC16O9_06700"
     CDS             complement(8941..9390)
                     /gene="nudB"
                     /locus_tag="AC16O9_06700"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HIR6516341.1"
                     /translation="MSLKLPESVLVVIHTPQLEVLLLERAQRPGFWQSVTGSLERLDE
                     APAHAARREVREETGIEAAPAQLIDWRMTHAFEIYAHWRGRFVPGTTHNTEHVFSLCV
                     PAPVTVELARAEHRAQRWLSWNRAADACFSWSNRDAIRMLPVRGARR"
     gene            complement(9387..9821)
                     /locus_tag="AC16O9_06705"
     CDS             complement(9387..9821)
                     /locus_tag="AC16O9_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HIR6516342.1"
                     /translation="MGKLAQRLKDATRSGVYRTRQATEVEDAVRGTRLDLALVDLGGA
                     HDKESLLERLALALGFPAWFGGNWDALEDCLGDLGWRAGDGHVFLFAGYENLPADDLG
                     VLLDVLAASAAYWAERSKPFFAVFVDAGRTLALPELFRAKSA"
     gene            complement(9825..10142)
                     /locus_tag="AC16O9_06710"
     CDS             complement(9825..10142)
                     /locus_tag="AC16O9_06710"
                     /EC_number="4.6.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046589 - ribonuclease T1 activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanyl-specific ribonuclease"
                     /protein_id="HIR6516343.1"
                     /translation="MAWTLVLAFAAGACSGEVALADLPPEARQTLALIKAGGPFPYAR
                     DGATFRNREGRLPERRPGYYREYTVRTPGARDRGARRIVAGGDGEFYYTQDHYRTFRR
                     IRE"
     gene            complement(10145..>11756)
                     /gene="aspS"
                     /locus_tag="AC16O9_06715"
     CDS             complement(10145..>11756)
                     /gene="aspS"
                     /locus_tag="AC16O9_06715"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR6516344.1"
                     /translation="DAFAAADRARGEYVLRVTGVVRHRPEGTVNPNLPSGEIEVFAHA
                     VEVLNPSVTPPFQLDDDTLSEGVRLEHRVLDLRREPMQKNLRLRHRAASAARAFLDAH
                     GFVDIETPVLYKSTPEGAREFLVPSRLHDGYFYALPQSPQLFKQMLMIAGFDRYYQIV
                     KCFRDEDLRADRQPEFTQIDVETSFMDEAAIRTLMEQLARHLFVEVLGVALPDPFPVL
                     RYEEVMRDYGVDKPDLRVPLKLAELTDVMRDVDFKVFSGPANAADGRVAALCVPGGGA
                     LTRGEIDAYTEYVKTLGAKGLAWIKVNERSKGAAGLQSPIVKNLNDQALAAILERSGA
                     ADGDLIFFAADKAAVANACLGALRQRIGHERGLAERGWRPLWVVDFPMFEYDDEAKAW
                     KARHHPFTSPKDGHEQFLESAPEKAYAKAYDLVLNGWEVAGGSVRIHRQEVQQQVFRG
                     LKISAEEQRAKFGFLLDALQYGAPPHGGIAFGFDRLVALMSGAEAIRDVIAFPKTQRG
                     QDLLTGAPSAVTERQLRDLHIRLRNPPQ"
BASE COUNT         1797 a         4160 c         3883 g         1916 t
ORIGIN      
        1 ccaccgtact tcttggccaa aatgtgcgtc atcgccgccg tcagcgtcgt cttgccgtga
       61 tccacgtgcc caatcgtccc cacgttcacg tgaggcttcg tacgctcgaa tttgcctttt
      121 gccatgatcc gaaatccccg agtcttgtgg ttacttgctt cttgccgtga tcaccgcctc
      181 ggcgacgctc ttcggcgctt cggcgtagtg cttgaattcc atggtgtagg tggcgcggcc
      241 ctgcgtgagc gagcgcagcg tcgtcgagta gccgaacatc tcgccgagcg gcacttcggc
      301 cttgatggtc ttgctgcccg ccaggtcgtc catgccctgg atgacgccac ggcgcgccga
      361 caggtcgccc atcacgtcgc ccatcttctc ttccggcgtc tccacttcga ccgccatgat
      421 cggctcgagc agcactggcg tcgccgcgcg catgccgtcc ttgaaggcga agatggccgc
      481 catcttgaac gcgttctcgt tcgagtccac ctcgtgatac gagccgtcga acagcgtcgc
      541 ctttacgtcc accaccgggt agccggcgag cacgccgtcc tgcagcgcct cgtccacgcc
      601 cttcttcacc gccgggatga attcgcgcgg cacgcggccg cccttgatcg cgtcgacgaa
      661 ctcgtagccc ttgcccacgg gctgcggctc gagcttgagc cagacgtggc cgtactggcc
      721 gcgcccgccc gattgcttga tgaacttgcc ctcggagtcg agcgacttct tgaacgtctc
      781 gcggtacgcg acctgcggct tgccgaccga ggcctcgacg ccgaactcgc gcttcatgcg
      841 gtcgacaatg atctccaggt gcagctcgcc catgcccgag atgatggtct gccccgactc
      901 ctcgtcggtg cgcacgcgga acgacgggtc ttcctgcgcc aggcgattca gcgcgacgcc
      961 catcttttcc tgatcaacct tggtcttggg ctccaccgcc tgcgagatca ccgggtccgg
     1021 gaactccatg cgctcgagcg tgatgacctt gttcacgtcg cagatcgtct cgcccgtgac
     1081 cacgtccttc aggccgacca cggcggcgat gtcgccggcg cgtacttcct tgatctcctt
     1141 gcgctcgttg gcgtgcatct gcagcagtcg cccaatgcgt tccttcttgc cgcgcaccga
     1201 cgtgtagacg gtgtcgcctg aggtcagcac gccggagtag acgcggatga acgacagctg
     1261 gccgacgaac gggtcggtca tgatcttgaa tgcgagcgcg gcgaacggct cgtcgtcgcc
     1321 cggcttgcgc acgtcgggct cgcccttgtc gttctcgccc ttcaccggcg ggatgtcgac
     1381 gggcgagggc agatagtcga tgacggcgtc cagcatcgcc tgcacgccct tgttcttgaa
     1441 cgccgagccg cacagcatcg gcacgatctc gttgttgatc gtgcgcagcc gcagcccgcg
     1501 cttcacttcc tcggtcgaca gctcgcccga ctcgaggtac ttgttcatca gcacttcgtt
     1561 cgcctcggcg gccgcctcga ccatcttctc gcgccactcc ttggcctgcg cggtgagatc
     1621 cgccgggatc ggcttcctct cgaacttcat gccctgcgtg gcgtcgtccc agtagattgc
     1681 gtccatcgtc accaggtcga ccacgccggc gaacttctcc tcggcgccaa tcgggatctg
     1741 gatcggcacg gggttgccct tcagccggtt gcggatatgg tcgtaggact tgaagaagtt
     1801 ggcgccgacg cgatccatct tgttgatgaa cgcgagccgc ggcacacggt acttgttcgc
     1861 ctggcgccac acggtctcgg actgcggctg cacgccggcc acggcgtcat acaccatgca
     1921 cgcgccgtcc agcacgcgca tcgagcgctc gacttcgatc gtgaagtcga cgtgcccggg
     1981 cgtgtcgatg atgttgatgt ggtgctcggg ataggactgg tccatgccct tccagaagca
     2041 ggtggtggcg gccgaggtga tggtgatgcc gcgctcctgc tcctgctcca tccagtccat
     2101 gacggcggcg ccatcgtgca cctcgcccat cttgtgcgac acgcccgtgt agaacaggat
     2161 gcgctcggtg gtcgtggtct tgccggcgtc gatatgcgcc gagatgccga tgttccggta
     2221 ccgctcgatg ggagtcttgc gtgccacgat actcaccaga atgcctctag aacctgtagt
     2281 gggcgaacgc tttgttggcg tccgccatgc gatggacttc ctcgcgcttc ttcattgcgc
     2341 caccgcggcc ctcggcggcc tcctgcagct cgcctgccag gcgcacggtc atggacttct
     2401 cgccgcgctt ttgcgccgcc tcgcggatcc agcgcatcgc gagcgccacg cgccgcaccg
     2461 ggcgcacttc caccggcacc tggtaattgg ccccgccgac gcgccggctc ttcacctcga
     2521 ccaccggctt cacgttctga accgcctgcg tgaacacctc gaccggatcc ttgcccgatt
     2581 tggtcttgat gacgtcgaac gcgcggtaga cgatgccctc ggccacggat ttcttgccgc
     2641 gcgtcatcag cacgttgatg aacttggcga cctccggatg attgaacttc ggatccggca
     2701 ggatctcgcg cttgggaacc tctctgcgac gcggcatggg cagtttctct ctgtgcttcg
     2761 ctaggccgtc ttgggcgcct tggcgccgta cttggagcgg ctctgcttgc ggtccttgac
     2821 gccctgcgtg tcgagcgagc cacgcacgat gtggtagcgc acgcccggca ggtccttcac
     2881 gcggccgccg cggatcagca ccaccgagtg ctcctgcagg ttgtggcctt caccgccgat
     2941 gtagccgatc acctcgaagc cgttggtgag gcgcaccttg gcgaccttgc gcagggccga
     3001 gttcggcttc ttcggcgtgg tcgtgtacac gcgcgtgcac acgccccgct tctgcggcga
     3061 gccggcgagc gcgggaacct tcgacttcgg gcgtcgagcg gcccggattc cacgccgtaa
     3121 ctgattgacg gtaggcatta ttttgcgatc ctagaaacaa accgggcggc cacgtccagc
     3181 gaacccgcag ccgcccaggc gccaaaaaag aggccggatt ataaggagga aaccgggtca
     3241 gcgcaaggaa tgagatggcg ttgcgacgaa gccaaagcgc gccgccaccg cgtgcgaggc
     3301 cacctcgggc agcgcgcgcc aattgtcgcc gagccgcgct gcaaatagcg cgaggtcgcg
     3361 gtcatcgagc agccccacgc cgcgctcgtc ctcgatcagc accgagcctt cctcgtcgac
     3421 aaacgtggca cgcgcgcgca cggggcgccc gcagtggtcg acgagcgttt cgccctcgta
     3481 gcgcaacacg agcggcgtat aggcgagcgc gacgaagacg cgctgggggc cgttctggaa
     3541 gtaccagcac ccgcgttcat cgtgcgcgta gttgcgggca atgaagtcgc gcaacatggc
     3601 gttgccgatg cgcccgaaac ctccgcccgc ggaatcttgc agcagccagt tgccgcggcg
     3661 gtcgagcgcg agccagccgt acaccgccgg cacatccggc catcgcgcca tcgctcgccg
     3721 cacgctcgcg tccatgccgg ccgcatcaag gcacgggcgg cgccgcggag cgatcgatgg
     3781 gcgccggccg cgcgatgccg aagccctgcg cgtacccgac acccaggcgg cgcagttgcg
     3841 cgacgatctc tggttcctcg acgcactcgg cgatcacctc gacaccgatc gcttcgccga
     3901 cccgcaccac cgccttcatc ttctggcgtg cgacttcgct gcgcagcagc gcgcgcacga
     3961 tggagccgtc gaccttgacc aagtccacgc gcatcgtctt caacgcaatg aacgagaccg
     4021 agcgctggcc gaatccgtcg atcaggacct tgcagccggt ctgtttcacg gcgtgggcga
     4081 aacgcgcggc ggcttcgagc cgggatagcg tgtcggcctc gtccacttca aacacgaggg
     4141 ccgccggcga cacgcgctgc gccagcagct cctcggcgac gaaccgcgga aacgccgcat
     4201 ccgccagagc ctggccggac acgttgaggc tcaggcgcgg aacgcccccg gccggcgggt
     4261 gcgccagcca gcgcaccgcg tgacgcacca cccagcggtc cagctcgccc atcatccggt
     4321 agtgctcgaa agcgggcagg aaatccccgg gcggcagcag cagcgcctct tcctcgcgca
     4381 ggcgcaccag cgcctcggcc atggcgaagg cgccgggcgg acgcagcgac agaatcggct
     4441 ggcagaacag ctgcagctcg tcgcgcgcca gcgcctggcg cagtcgcgcc gcgggatcgc
     4501 gccagcccgc cagctcgcgg ttcatctcct ctaggaactt ctcctcgttc gccgccgcgg
     4561 aggatcctgc agtgctcgtc gtcatgccct cgcccctaaa gtattctagc cgcgcgcgcc
     4621 gcttggtcga gctaacattg cgccggttta tcctggagac cgacagcaat gccccgagag
     4681 gattttgcct gcgcgcaccg gctgcgcgta cgctgggccg aggtggacat gcaaaggatc
     4741 gtgttcaacg gccactatct cacctacatc gacacggcaa tcgccgagta ctggcgcgcc
     4801 atcgggttgc cctaccccga aggctatgtc gagcgctatg cgagcgacct gttcctgcgc
     4861 aaggcgacgg tggagtacct cgggtccgcc ggctacgacg acgagctcga agtcctgtgc
     4921 cgcgcctcgc gcctcggacg cagcagcatg acgttcctgt tcgagatctg gcgcggcgca
     4981 tccgagctgc tggtcaccgc ggagctggtc tacgtgaacg ccgacccgaa ggccaagacc
     5041 gcggcaccgc tgcctgacga ggtacgcgag cgcatccgcg gtttcgagaa gtcagggccc
     5101 gcgcaatgac gctgtactgg gaggatttca acgtcggcga caccgtggag atggggcgcc
     5161 atacctttac cgaggccgag atcatcgcgt tcgcgcgcca gttcgacccg cagccgtttc
     5221 acgtcgatcc gcaggcggcg atgcaatcgg cctttggcgg gctgatcgcg agcggctggc
     5281 acacgtgcgc ggtgtgcatg cgcctcaatt gcgagaccta tatcaaccgc tccgtcagcc
     5341 tgggctcccc cggcctcgac aacattcgct ggctgaagcc cgtgcgcccg ggcgacacca
     5401 tcagctaccg gcgcacggtg ctcgaatcgc gcgcttcgca gagccggccg ggcgtcggcc
     5461 tgttgcgctc gcgcaccgaa gcgctcaacc aggcgggcga gctcgtcatg tcgatggaag
     5521 gctggggcat gttcggtcgg cggccggcgc cgtgagcgcg aagatcgagc tgcagcaccg
     5581 catccacgtg ctcacgcgca aggaggagct cgacggcgtg cgcgtcgcgg gcaaggtggt
     5641 catcgtgctc gatatcctat tcgccacgac caccatggtg gcggtgctcg ccggcggcgc
     5701 cacggaagtg ctgccagtgg tcgacgagag cgcggcgcgc gatgccggcg cgcggcacgc
     5761 cgaagggggc tacgtgctcg ccggggagct gtacgccgag acgctgccgg gattcgcgca
     5821 tccggcgccg atggcgctgc tggcacacgg cgtcgccggc aaaagcgtga tctacgccac
     5881 caccaacggc accgtggcga tgacgctggc cgttggcgca aagcgcgtgt actgcggctc
     5941 gctgctcaat gccggcgcgc tcgtttcgcg tgtcgtggcc gagcacccgc aggaccgggt
     6001 gctgatcctg tgctcgggct cgggtggcaa cttcaacctg gaggatttct acggcgcggg
     6061 ctgcttcgtc gagcgtttca cgcgactgct cgggcccggc gcggacttgt ccgatgcggc
     6121 gcgcacctcg ctcgccttct ttcgcggcgc tcgtgcgccc gaggcgctgc tcgagtgccg
     6181 cgtcggccgc atgatggccg cgcgcggcct cgcgcacgag gtcgagttcg cctgccgcgt
     6241 cgatgccttt cccattgtgc ccgtgctgga gacaggccgc ctgcgatgcg cgtagccgcg
     6301 ctcgccgtct atccggtgaa aggcgcggcg gcggtgccgc tggagcgcgt ggcgctcgac
     6361 aacatggggc cgctcggcga tcggcgctac tgcctcgcac gcccgagtgg gcgcgtgttc
     6421 acgcagcgcg acgacgccag tctggcgcgg ctgagcgcgc aaccgcacga cggcgggctg
     6481 ctgctcgagt tcgacggtga gcggctgcag gtcggcgcgg ccgacttcgc cactgcggcc
     6541 cagctgcacg tctggtcgag gcagactgcc gcgcgcatcg cgcgcaatgc gctcaatgcg
     6601 gccctcacgc gctggttcgg gacgccgctg cagctggcgc gcctcgagcg gccggtggag
     6661 cgccagggcg ccatgcttgc cttcggcgac gccgcggcgc tgctgctgac caacgcggct
     6721 tcgctggacg cgctcaacgc cagcatggac gttgcggtgc cgatggcgcg cttcaggccg
     6781 aacatcgtcc tcgcgggcgc cgccgcgttc gatgaggacc gctggcagcg cctgcgcgtc
     6841 ggcagcgcga tccttgcgcc ggtgcacgcc tgcggccgct gcgaagtcac caccatcgac
     6901 caggaggtgg gcgccgtcgt ggacggcgag ccgctgcgca ccctttcgcg cctgcgcatg
     6961 cgggacggcg agccggtgtt cggcgtgcgc tacgcagtgg cgcagccggg cgacatccgc
     7021 gtcggcgacc gggcgttcga cgcaccctag tcctggtcag tgatagcgct catagcccgc
     7081 gaacgcggtc agcaggcggg tgatggagta tgcgacccag atcggcatcc gctgccatag
     7141 cggccggcgc cgccagtgct ggggcggaat ccgctgcgcg ccgttgcgca tcgcctcttg
     7201 cagccccccg cgcagttgcg cggagaagcg ccggtcgtcc acgaacacgt tcgcctccag
     7261 cgccatgagc aggctgtagg gatcgatgtt cgaggagccg acgcagcaat gcacgccgtc
     7321 gaacaccgcc accttggcgt gcaggaagct gcgctggtac tcccagatct cgatgccggc
     7381 atcgagcagc tggccgtaca tggcacgcga ggcgagatgc agcagcaggt attcgacgcg
     7441 gccctgcagg aacagcacca cccgaacgcc gcgccgcgcc gcggcgacca gcgcccggcg
     7501 gaaacgccag cccggcagga agtaggcatt ggcgatgatc acctcgtcgc gggcagccgc
     7561 aattcgctcg aggtagacgc gctcgatgtc gcgccggtgg tgcaggctgt cgcgcaccac
     7621 caaggcggcg cgatgaccct ccgcaaacgg gccgcgcgcc ggcacgggcg cgcgcggcgc
     7681 ctccccggcg aggcgcgccc acagccgcgc tgcgcgctcg cgaacctgcg ccgtgagcgg
     7741 gccctcgatg cgcaccgcat agtcaagccg cggcggcgac ggcatcggtc cgacgtagtc
     7801 gtcaaggata ttgatgccgc cgacgaacgc cagctggccg tcgatgcacg cattcttgcg
     7861 atgcagccgg cgcagccgat cgcgccgcaa gccccagatg cgcaccggcc ggtacatgtg
     7921 cagctgcacg cccgtgtcct cgaggatgcg gcgaaaacgc ggcggaaagc cgcgcgagcc
     7981 gaaaccgtcg atcagcaggc gcaccgccac cccgcgcgcc gcggccctgg cgagcgcgtc
     8041 gatgacgagg ctgccggtct cgtcgccggc gaagatgtag gtctcgagga acacttcgcg
     8101 ccgcgcgccg tcgatcgcct cgacgagcgc cgggaagtac tgctcgccgt tgcacagcag
     8161 ggtcagccgg ttgccgtcga catagcgcat ggcgctaggc agccttcacc accgcgccca
     8221 gcgccaggtg atccgagagc agcgaccagg gcgcgccgac gtgcacgctc gcctcgacga
     8281 cgctgaagcc gcgcacgtag atccggtcca ggcgcagcac cggcagcagg ctcggatagg
     8341 tgcgcgccgg ccgatcccca tagatggcgg tcacctcctg catgccgagc cgctcctcga
     8401 gcacgcgcgt cgcgcgctgg cgccagtcgt tgaaatcccc ggcgacgacg atcggctcgc
     8461 cgcgtgccgc cgcctcgccg aggcgctcga cgatggcctc gagctggcgc cggcgcccgc
     8521 gctcgtgcag cgaaaggtgc acgcagacgc aatgcagctc gcgccgccag cccggaacgc
     8581 tcagcacgca atgcagcagg ccgcggcgct cgaagcgatg gctcgaaatg tccacgttct
     8641 ccgtcgacac gatcggaaac cgcgacagca cggcgttgcc gtaatgtccg tagtgatgta
     8701 cacggttcag gccgtaggcg tactgccagc cttcctgttt cagatactcg tgctgcggtg
     8761 cgctcggcca ggtcgcgaag cgcagcgcgt ggcgctcgtt gaggccctga acctcctgca
     8821 ggaacaccag gtcgggctcg agcgtgttca ggccttcgcg cagctcatgg atcaccacgc
     8881 gccggttgaa ttgcgagagc cccttgtgca tgttgagcgt gaccacacga aggaccgcgc
     8941 tcatcgccgc gcgccccgca ccggcagcat gcgaatggcg tcccggttcg accacgagaa
     9001 gcaagcgtcg gccgcccggt tccaggacag ccagcgttgc gcgcggtgct ccgcccgcgc
     9061 cagctcgacc gtcaccggcg ccggcacgca caggctgaac acgtgctcgg tgttgtgcgt
     9121 cgtgccgggc acgaagcggc cgcgccagtg cgcgtagatc tcgaacgcgt gcgtcatccg
     9181 ccagtcgatc agctgcgccg gcgccgcctc gatgccggtt tcctcgcgca cttcgcgccg
     9241 cgccgcatgc gccggcgcct cgtccagccg ctcgagcgag ccggtgaccg actgccagaa
     9301 gccggggcgt tgcgcccgct ccagcagcag cacctccagc tgcggcgtgt ggatcaccac
     9361 cagcaccgat tccggcagct tgaggctcac gcggacttcg cgcggaacag ctccggcagc
     9421 gcgagcgtgc gtcccgcatc gacgaacacc gcaaagaacg gcttgctgcg ctcggcccag
     9481 tacgcggcgc tcgcggccag cacgtcgagc agcacgccca ggtcgtcggc gggcaggttc
     9541 tcgtaaccgg caaagaggaa gacatggccg tccccggcgc gccagcccag atcgccgaga
     9601 cagtcctcca gcgcgtccca gttgccgcca aaccacgccg gaaagccgag cgccagcgcg
     9661 agccgctcga gcagcgattc cttgtcgtgc gccccgccga gatcgacgag cgccaggtcg
     9721 aggcgcgtgc cgcgcaccgc gtcctcgact tccgtggcct gccgcgtccg gtacacgccg
     9781 gagcgcgtcg catccttaag cctttgcgca agcttaccca tgacctattc gcggatgcgc
     9841 ctgaatgtcc ggtagtggtc ctgcgtgtag tagaactcgc cgtccccgcc cgccacgata
     9901 cgccgcgcgc cgcggtcgcg cgcgcccggc gtgcgtaccg tgtactcgcg atagtagccg
     9961 ggccggcgtt cgggcagccg gccctcgcgg ttgcggaacg tcgcgccgtc gcgcgcgtag
    10021 gggaacgggc caccggcctt gatcagcgcc agcgtctgcc gcgcctcggg cggcaaatcg
    10081 gcaagcgcga cttcgcccga gcaggcgccc gcggcgaacg ccagcacgag cgtccaggcc
    10141 agcctcactg cggcgggttg cgcagccgga tgtggaggtc gcgcagctga cgctcggtga
    10201 ccgcgctggg cgcgcccgtc agcaggtcct gtccgcgctg cgtctttgga aaggcgatca
    10261 cgtcgcggat cgcctccgcg ccgctcatca gggccaccag ccggtcgaag ccgaaggcga
    10321 tgccgccgtg cggcggcgcg ccgtactgca gggcatcgag caggaagccg aacttcgcgc
    10381 gctgctcctc ggcggagatc ttcagcccgc ggaacacctg ctgctggact tcctggcggt
    10441 ggatgcgcac cgagccgccc gccacttccc agccgttcaa gaccaagtcg taggccttgg
    10501 cgtacgcctt ttccggggcc gactcgagga actgctcgtg gccgtccttg gggctggtga
    10561 acgggtgatg gcgcgccttc caggccttcg cttcgtcgtc atactcgaac atcgggaagt
    10621 ccaccaccca gagcggccgc cagccccgct cggcaaggcc gcgctcgtgg ccgatcctct
    10681 ggcgcagcgc gccgaggcag gcattcgcca ccgccgcctt gtcggcggcg aagaaaatga
    10741 gatcgccgtc tgctgcgccg ctgcgctcca ggatcgccgc gagcgcctgg tcgttcaggt
    10801 tcttgacgat cggcgactgc agccccgcgg cgcccttgct gcgctcgttg actttgatcc
    10861 aggcgaggcc cttggccccc agcgtcttga cgtactcggt ataggcgtcg atctcgccgc
    10921 gtgtcagcgc cccgccgccg ggcacgcaca gggccgcgac gcggccgtcg gccgcgttcg
    10981 ccgggcccga gaacaccttg aagtccacat ctctcattac gtcggttagc tcggcgagtt
    11041 tgagcggcac gcgcaagtcc ggcttgtcga cgccgtagtc gcgcatcacc tcctcgtagc
    11101 gcagcaccgg gaacggatcg ggcagcgcca cgccgagcac ctccacgaac aggtggcgcg
    11161 ccagttgctc catcagcgtg cggatcgccg cctcgtccat gaacgaagtc tcgacgtcga
    11221 tctgcgtgaa ctcgggctgg cgatcggcgc gcaggtcctc gtcgcggaag cacttcacga
    11281 tctggtagta gcggtcgaac cccgcgatca tcagcatctg cttgaacagt tgcgggctct
    11341 gcggcaacgc gtagaagtag ccgtcgtgca ggcgcgaggg caccaggaac tcgcgcgcgc
    11401 cctcgggcgt ggacttgtac agcaccgggg tctcgatatc gacgaagccg tgcgcgtcga
    11461 ggaaggcgcg cgccgccgat gccgctcgat ggcgcaggcg caggttcttc tgcatcggct
    11521 cgcgccgcag gtcgagcacg cgatgctcca ggcgcacgcc ctccgaaagg gtgtcgtcgt
    11581 cgagctggaa cggcggcgtc accgaaggat tgagcacctc caccgcatgc gcgaacacct
    11641 cgatctcgcc cgaaggcagg ttcgggttga ccgtcccctc ggggcggtgg cgcaccacgc
    11701 cggtgacgcg aaggacgtac tcgccgcgcg cgcggtcggc ggcggcgaag gcgtcg
//
LOCUS       DBNHKE010000108        17565 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1182328, whole genome shotgun sequence.
ACCESSION   DBNHKE010000108 DBNHKE010000000
VERSION     DBNHKE010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1182328"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /gene="treZ"
                     /locus_tag="AC16O9_06720"
     CDS             complement(<1..1794)
                     /gene="treZ"
                     /locus_tag="AC16O9_06720"
                     /EC_number="3.2.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033942 -
                     4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HIR6516345.1"
                     /translation="MRRAHDMPFGAAPRGDGQFAFRLWAPGEKQVVLEVGEATAPMTR
                     TAAGWHELTLPASAGARYRYRLADGVAVPDPASRFNPEDVHAASQLVDPHEYLWRDAG
                     WRGRPWEEAVVYELHVGTFTPQGTFAAARERLADLAALGITAIELMPVADFPGARGWG
                     YDGVLHYAPDASYGTPNDLKALVDAAHGHGLMVLLDVVYNHFGPDGNYLHAYCPQFFN
                     AKRQTPWGAAINFDGPESRAVRDFYVHNALYWVEEYAFDGLRLDAVHAIRDDSALHIV
                     REIAQALAAGPGRERHVHLVLENGANEAHLLERGPDGAPRCATAQWNDDIHHAIHVIT
                     TGEKDGYYVDFADAPVAQLGRALAEGFIFQGQVSPFHDHQPRGEPSAHLPGGAFVSFL
                     QTHDQVGNRAFGERIHALATDQRLLRAAYACVLLSPHAPMLFMGEEYAASTPFQYFCD
                     FHDELADAVRNGRREEFKGFAAFRDEAARARIPDPNAESTLAASTLQWGERERSPHRE
                     WLAYVGELLRLRRQHLVPRLAGPRGGGRFACEGALLRVEWTLGDGSRWRLLANFGSGD
                     AESQANAGTPVYTAGVKELSAGRLRLAPGAVR"
     gene            complement(1791..3929)
                     /gene="glgX"
                     /locus_tag="AC16O9_06725"
     CDS             complement(1791..3929)
                     /gene="glgX"
                     /locus_tag="AC16O9_06725"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR6516346.1"
                     /translation="MSEAPPLGAVWRGAPYPKGATWDGEGVNFALFSEHAEKVELCLF
                     EPSGTRELQRIEIRERTDNVWHCYLPEARPGQVYGYRVHGPYRPKDGHRFNGYKLLID
                     PYARELVGEVRWNDALYGYTIGAAEADLKADERDSAAFMPKCRVVDTAFTWGDDRRPS
                     VPWHDMVIYELHVRGFTKRHPEVPEPQRGTYAGLCSAPVIEYLKRLGVTTVELMPVHA
                     FIDDRRLVEQGLRNYWGYNTLGFFAPEPRYSASGSSKEFKTMVKRLHSAGIEVIIDVV
                     YNHTCEGNHLGPTLAFRGVDNASYYRLMEDERRHYLDFTGCGNTVNLEHPRVLQMVMD
                     SLRYWVEEMHVDGFRFDLAPALAREGRAVEQLGGFFSVVRQDPVLNRVKLIAEPWDIG
                     EGGYQVGNFPLGWAEWNDRYRNGMRAYWKGDGGVIGEFARRLTGSSDLYGRGGRRPFA
                     SINFVTAHDGFTLHDLVSYNDKHNEANGEDNRDGHNENLSWNCGVEGPTDDPEVNELR
                     DRQKKNFLATLLLSQGVPMLLAGDERGHSQNGNNNAYCQDNETSWIDWRHTPQRAALA
                     EFTRLLIMLRRSHRVFRRRDFFEGRPLHGTDVKDIQWLRPDGAEMTDQEWDHEHARSL
                     GVYLAGGDLGELDRYGMPATDDDFLILFNAHSEPVEFRFPVSRWPWHALVDTAVAPLE
                     TPAGRFTPDKPYRLAARSLVLLTRTSAHAP"
     gene            complement(3946..4617)
                     /locus_tag="AC16O9_06730"
     CDS             complement(3946..4617)
                     /locus_tag="AC16O9_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6516347.1"
                     /translation="MDINVFLEPMRAVLTQIGVFLPKLGLALVVLIVGWLLARLARFT
                     VAKALRAINFNVLTERAGMDGFLKQGGIQSDTTDIFAVLVYWLVIFIALVIAFNGLGL
                     TYITDLLGQVVLFVPKVIVALLLFAFGGYFAMFVGNTVATYCGNVGIKDGDLLGKLAQ
                     YAIIAFVVLMALDQVNIGGDVVRHSFLILLAGVVLGLALAFGIGGQKWAAGLLERWWP
                     RGRGE"
     gene            complement(4636..7968)
                     /gene="treS"
                     /locus_tag="AC16O9_06735"
     CDS             complement(4636..7968)
                     /gene="treS"
                     /locus_tag="AC16O9_06735"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HIR6516348.1"
                     /translation="MNAPTTTTTSAPAAAGQAAAPAAADPHWYKDAVVYQLHIKAFYD
                     SNGDGLGDFRGLTEKLDYVRDLGVNTIWLLPFYPSPLKDDGYDVADYREVHPQYGNAE
                     DFRVFVEEAHKRNLRVITELIVNHTSDAHPWFQAARRAPKGSPERDFYVWSDDDQKYK
                     GTRIIFTDTETSNWTWDPVAKQYFWHRFFSHQPDLNFDNPKVLEAVLEVMAFWCELGV
                     DGFRLDAIPYLVEREGTSNENLPETHAVIKRIRAALDARYPGKLLLAEANQWPEDVRE
                     YFGEADECQMAYHFPLMPRMYMAIAQEDRHPVVEIMQQTPEIPESCQWAIFLRNHDEL
                     TLEMVTSKERDYMYRTYAADTRARINLGIRRRLAPLLENDIDRIKLMNSLLLTMPGSP
                     IIYYGDEIGMGDNIYLGDRNGVRTPMQWTPDRNAGFSRADPQRLYLPPIMDPIYGFEA
                     VNVEAQSREPNSLLNWMRRLLAVRKTSHAFGRGKLQFLRAGNRKVLAYVREYGDEVIL
                     CVANVGRTAQPVELDLRRFKGRVPVELMGRTVFPPIGELPYLLTLPGCGFYWFRLATD
                     AAAPVWHAEVLAREDLPMLVMFDGWTSFFRDRVVPWRINMAVKTREHFETQVLPGWLE
                     TQRWYAAKGDTGARVRLGDWALWEEAGRATYMLPLLEVEGGAEAARYFAPLTLAWEDR
                     EEERVKALGGAAVAKVRQQASVGIMADAFADEAFCRAVVEAIGAGRELAGAQGKLRFT
                     PTAAFAEVCSEDVNLLPVGRPKAQSSNTVVTLGERMFLKGYRRIRPGVNPEFEVGRFL
                     TEKVRYANCVPVAGAVEYTAADGTPMTLALLQAYVPNQGDGWNHTVEYLERFLENQRA
                     SGAAGGDHGIYLPLMQTLGTRTGELHCALATRTGDAAFDPEPVTDRDFSQWKAGIRTE
                     AAKTLDMLAARRGQLTAPARDDAAELVESRERLLRRIEGYRLPKGATQKTRFHGDYHL
                     GQVLLQQLDFVITDFEGEPARSIEERRAKHSPLKDVAGMLRSFNYARWTALRQALEGH
                     NDYERLAPFAEDWEARVRRTFLGAYEQAARASGLYASFHDMHELLELFELEKALYELR
                     YELGNRPGWVGIPLQGILALAR"
     gene            complement(7973..9982)
                     /locus_tag="AC16O9_06740"
     CDS             complement(7973..9982)
                     /locus_tag="AC16O9_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HIR6516349.1"
                     /translation="MSPTGASDGRVRAVIDAVRPAVDGGRFAVKRVAGEALEVEAHCF
                     ADGHDVLRARLLWRAEDARDWQEVEMKPLGNDVWRAQFTPKEIGRYRYSAAAWVDAFA
                     TWRHDFARREDPADQALAALAGAYLAEEAAARAKHENAGADAQQLKDWAARLRAAKKP
                     VEIRATALDEVMAALAARYPDRSLEARWGVDMPLVVDRERARYSTWYELFPRSASAEP
                     GKHGTFKDVEKRLAYVAELGFDVLYLPPIHPVGRERRKGKNNAVTAGADDLGSPWAIG
                     AKEGGHKSILPALGTPEDFRSLVKAAAKHGIEIALDIAFQCAPDHPYVKEHPDWFRKR
                     PDGSVQYAENPPKKYQDIYPFDFECADWKALWQELKSVFDFWIGEGVKIFRVDNPHTK
                     AFPFWEWAIAEIRRAHPDVLFLAEAFTRPKVMHRLAKLGYTQSYTYFAWRNTKQELTE
                     YFTELAQGPGKEYFRPNVWPNTPDILTEALQVGGRAVFMQRLALAATLSASYGIYGPA
                     YELMEHLPREPGSEEYLHSEKYQLRHWDLERADSLRAYIAAFNRIRRENPALQSDAGL
                     RFCNTDNDQLLAYSKQAADGSNLIVCVVNLSPHHTHGGWVELDLGALGIEPDRPYQMH
                     DLLSGARYMWNGPRNFVQLDPQRSPAHVFRVRRRLHREQDFDYFL"
     gene            complement(9979..11853)
                     /gene="glgB"
                     /locus_tag="AC16O9_06745"
     CDS             complement(9979..11853)
                     /gene="glgB"
                     /locus_tag="AC16O9_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR6516350.1"
                     /translation="MLTEHDAYLFREGTHARLYERLGCHPDGKKKGAHFGVWAPNARA
                     VSVIGDWNGWRAGADMLAPRGDHSGIWEGSVRGAERGQAYKFRIESHVGGHVSEKADP
                     FAVAAELPPATGSRVWTLEYDWGDAEWMKTRGPKNALEAPMSVYEVHAGSWKRKNGAF
                     LGYRELAHELAAYVKEAGFTHVELMPLTEHPFYGSWGYQTTGYFAPTARYGTPQDFMY
                     LVDHLHQAGIGVILDWVPSHFPADAHGLGFFDGTHLYEHADPRQGFHPEWNSSIFNYG
                     RNEVRSFLLSSALFWLDKYHIDGIRVDAVASMLYLDYARKHGEWIPNKYGGRENLEAI
                     SFLRTLNEGVYRDHPDCFTAAEESTAWPMVSRPTYLGGLGFGLKWNMGWMHDTLAYFK
                     EDPIHRKYHHGRLTFSLIYAFNENFTLPLSHDEVVHGKGSLIGKMPGDAWQQFANLRA
                     LYGYMWGHPGKKLLFMGGEFGQRREWTHDGELEWWVCERPEHAGLQRWVRDLNRAYRA
                     EPALWQVDFSHEGFEWIDANDAQNSAISFLRKPRGKGPLVAVVCNLTPLPRENYVLGV
                     PRGGTWREIVNSDAKEYGGAGWGNFGAVQAAPLAAHGRPHSLTLTLPPLSSVYLRSDT
                     "
     gene            12145..13209
                     /locus_tag="AC16O9_06750"
     CDS             12145..13209
                     /locus_tag="AC16O9_06750"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6516351.1"
                     /translation="MKLWEQLLSDNAPGAWLLAGAIVFAVLAAVWLARAIVARVVTRI
                     ARRTALQLDDALIAAVRATKLWLLTPLALYAGASALEIPPRLDRLLEHLAMVGLIAQF
                     AVWTNRWVLRWLALRADAARETDPAATTTVNLLGFVARVVVWALALLIALDQLGFNVT
                     ALVAGLGIGGIAVALAVQNILGDLFASMAIVLDKPFQVGDFIIVGDKPGTVEKVGLKT
                     TRVKSLWGEQYVFANAQLLNSQIRNYKKMRERRIAFGIGVTYETPAEKLRALPGWLKA
                     AVEAQPDLRFDRAHFKSYGDFSLDFEIVYYVLSPDYNLYMDRQQAINLAIFDKLAQEG
                     VHFAYPTRTLYLRPEPAAGR"
     gene            13220..14731
                     /locus_tag="AC16O9_06755"
     CDS             13220..14731
                     /locus_tag="AC16O9_06755"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR6516352.1"
                     /translation="MKGAGAGAGRERLIVVSNRLPFTFKKDGETWRSEPGGGGLVTAL
                     LPVLRDRGGMWIGWPGTTGDPKELDAALAAAGANAGYTLAGVPLTADEMDKFYLGFSN
                     EIIWPLFHDLQSLCNFDPAYWRTYCDVNAKYADVVKAHTAPGDFIWIHDYHLMNVAAE
                     LRRRGVRSRQGFFLHIPFPSPDLFMKLPWRLSLLQALLEFDLIGFQTALDRRNFVQCA
                     RMLVKDVQIEGRGQVLEARVGKRTVRIGAFPISIDYGAFQRRAAAPEVTAKAQELHRL
                     LPDRKLILGIDRLDYTKGIPLRLKAFQNLLERYPELREHVSFLQVVVPSREDIPEYHR
                     LRTEIEQLVGHINGRFARPGGWVPIWYEYRNLSRLELLAYYRAAHIALITPLKDGMNL
                     VAKEYCTCSIEEDCVLILSEFAGAASQLYRGALLVNPYDIEGVADTIQRAHGMHPEER
                     HARMRRMRNSIREHDVFWWVDSFLRAAITRELSSFPQPEGHPEEDATEYQLPF"
     gene            complement(14733..15989)
                     /gene="moeA"
                     /locus_tag="AC16O9_06760"
     CDS             complement(14733..15989)
                     /gene="moeA"
                     /locus_tag="AC16O9_06760"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR6516353.1"
                     /translation="MKRIIEIAQAIEGYDPDALHVDKARAAIRACLAPVKERERAPVR
                     AALGRVLAEDIVPAIDVPGHDNTAMDGYAVRHADLGAGATVLEEIGAALAGKAYAGTV
                     GAGQCVRVTTGAVMPAGTDTVVVQEVVKVHGTKVTIPAGQKAKQNVRYAGEDLKKGVP
                     VLSPGRLLAPAELGLIASLGIGEVAVRRRVRVAFFSTGDELASVGSALRQGEVYDSNR
                     YTLHGMLARLGVEARDLGVVRDDPRELESALRDAASNADAIITTGGVSVGEADFVKPM
                     MAKLGEALFWRIAMRPGRPMAFGRIGNAFLFGLPGNPVAVMVTFYQFVREALWQLAGR
                     SGAALPLLEARAADPLRKVAGRTEYQRGMLYREGAELKVRTTGQQGSGVLRSMSEANC
                     VIVLEHERGRVQAGEPVQVQPFDGLT"
     gene            complement(15986..16549)
                     /gene="mobA"
                     /locus_tag="AC16O9_06765"
     CDS             complement(15986..16549)
                     /gene="mobA"
                     /locus_tag="AC16O9_06765"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIR6516354.1"
                     /translation="MDAVSAIVLAGGQGRRMGGVDKGLVVLRGKPMVAWVLERLAPQA
                     GEIVINANQNLDAYGAFGYRVVPDAIGGFAGPLAGLHAGLAAISHPLAATVPCDSPFL
                     PLDLVARLRAALGAHDLAVAKTADQPHPVFSLVRRSVHAHLGAFLAAGGRKIDAWYAS
                     LKVVEVPFDDEADAFRNINTREELGQA"
     gene            16581..>17565
                     /locus_tag="AC16O9_06770"
     CDS             16581..>17565
                     /locus_tag="AC16O9_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6516355.1"
                     /translation="MNDRRWLVPFLNLGHLLDHLAMLVFPTAVLALGAAWNRPYSELL
                     PLALGGFIAFGAFAIPAGWLADHWSRYRVLAIFFFGIGASLFLTGLAREPWHIAVGLT
                     LVGAFAAIYHPVGIAMLVASPEKMGRVLGWNGLWGNLGLAFAALLTGALVDLAGWRAA
                     FFVPGLICIAAGVGFLAVVKDPGRVKKSSKSIGLHVDGRTMARIFTVLLVATACGGII
                     FNSTTVSMPKVFDERLRALTQTNFGIGALVAAVYTVAAFAQVAMGALIDRFELRRLMI
                     GVALAQIPLLALAANLQGWAMLGAAVLMMLAVFGQIPLNDAIVGRYCADEFR"
BASE COUNT         2564 a         6011 c         6138 g         2852 t
ORIGIN      
        1 gcggaccgcg cccggcgcca gccgcaggcg cccggcggac agctccttga cgcctgcggt
       61 gtacacgggt gtaccggcgt tggcctgcga ctcggcgtcg ccggaaccga agttcgcgag
      121 caaacgccag cggctgccgt cgccgagcgt ccactcgacc cgcagcagcg cgccttcgca
      181 ggcgaagcgg ccgccgccgc gcgggccggc aaggcgcggc acgaggtgct ggcggcgcag
      241 gcgcaggagc tcgcccacat acgccagcca ctcgcggtgc ggcgagcgtt cgcgctcgcc
      301 ccattgcagc gtcgaggccg cgagggtgga ctcggcgttc ggatcgggaa tgcgcgcgcg
      361 cgccgcctcg tcgcggaacg cggcgaagcc cttgaactcc tcgcgccggc cgttacgcac
      421 cgcgtcggcg agctcgtcgt ggaagtcgca gaagtactgg aacggcgtcg aggccgcgta
      481 ctcctcgccc atgaacagca tcggcgcgtg cggcgagagc agtacgcagg cgtacgccgc
      541 gcgcagcagt cgctggtcgg tggccagggc gtgaatgcgc tcgccgaagg cacggttgcc
      601 gacctggtcg tgggtctgca ggaacgacac gaaggcgccg cccggcaggt gcgcgctcgg
      661 ctcgccgcgc ggctgatggt cgtggaaggg cgagacctgc ccctggaaga tgaagccttc
      721 ggcgagcgcg cgccccagct gcgcaaccgg cgcatccgcg aagtcgacgt agtagccgtc
      781 cttctcgccg gtggtgatga cgtggatggc atggtggatg tcgtcgttcc actgcgccgt
      841 ggcgcagcgc ggcgccccgt cggggccgcg ctcgagcaag tgcgcctcgt tggcgccgtt
      901 ctcgagcacc aggtgcacgt gacgctcgcg ccccggcccg gcggcgagcg cctgcgcgat
      961 ttcgcgcacg atgtgcagag ccgagtcgtc gcggatggcg tgcacggcgt ccaggcgcag
     1021 gccgtcgaac gcgtactcct cgacccagta cagcgcgttg tgcacgtaga agtcgcgcac
     1081 cgcgcggctc tcgggcccgt cgaagttgat cgccgcgccc cagggcgtct ggcgcttcgc
     1141 gttgaagaat tgcgggcagt aggcgtgcag gtagttgccg tccggcccga agtggttgta
     1201 gaccacgtcg agcagcacca tcaggccgtg gccgtgcgcc gcgtccacca gcgccttcaa
     1261 gtcgttcggc gtgccgtagc tcgcgtcggg cgcgtagtgc agcacgccgt cgtagcccca
     1321 gccgcgcgcg cccgggaagt cggccaccgg catcagctcg atcgcggtga tgccgagcgc
     1381 ggcaagatcg gccaggcgct cgcgcgcggc ggcgaacgtg ccctgcggcg tgaacgtgcc
     1441 gacgtgcagc tcgtagacca cggcctcttc ccaggggcgg ccacgccagc cggcgtcgcg
     1501 ccacaggtac tcatgcgggt cgacgagctg gctcgcggcg tgcacgtcct cgggattgaa
     1561 gcgcgaggcg gggtcgggca ccgcgacgcc gtcggccagc cggtagcggt agcgcgcgcc
     1621 ggcgctcgcc gggagcgtca gctcgtgcca gcccgccgcc gtgcgcgtca tgggcgcggt
     1681 cgcttcgccg acctcgagca ccacctgctt ttcgccgggc gcccacaggc ggaaagcgaa
     1741 ttggccgtcg ccccgcggcg cggcgccgaa gggcatgtcg tgcgcccgcc tcatggcgcg
     1801 tgcgccgaag tgcgcgtgag caggacgagc gagcgcgccg cgaggcgata gggcttgtcg
     1861 ggcgtgaagc gcccggccgg ggtctccagc ggcgccaccg cggtgtcgac gagcgcgtgc
     1921 cacggccagc gcgacaccgg gaagcgaaac tcgaccggct cgctgtgggc gttgaacagg
     1981 atcaggaaat cgtcgtccgt cgccggcatg ccgtagcggt ccagctcgcc caggtcgccg
     2041 ccggcgagat acacgccgag cgagcgcgcg tgctcgtggt cccactcctg gtcggtcatc
     2101 tcggcgccgt cgggccgcag ccactggatg tccttcacgt ccgtgccgtg cagcgggcgc
     2161 ccttcgaaga agtcgcgccg gcggaacacg cggtgcgagc ggcgcagcat gatcagcagg
     2221 cgcgtgaatt cggcgagcgc agcgcgctgc ggcgtgtggc gccagtcgat ccaggaggtc
     2281 tcgttgtcct ggcaataggc gttgttgttg ccgttctggc tgtggccgcg ctcgtcgccc
     2341 gcgagcagca tcggcacgcc ttgcgacagc agcagcgtgg cgaggaagtt cttcttctgg
     2401 cggtcgcgca gctcgttgac ctcgggatcg tcggtcgggc cctcgacgcc gcagttccag
     2461 gacaggttct cgttgtggcc gtcccggttg tcctcgccgt tcgcctcgtt gtgcttgtcg
     2521 ttgtaggaca ccaggtcgtg cagggtgaag ccatcgtgcg cggtgacgaa attgatgctg
     2581 gcgaacggcc ggcggccgcc gcgcccgtac aggtccgacg agccggtgag gcgccgcgcg
     2641 aactcgccga tcacgccgcc gtcgcccttc cagtaggcgc gcatgccgtt gcggtaccga
     2701 tcgttccact ccgcccagcc gagcgggaag ttgcccacct gatagccgcc ctcgccaatg
     2761 tcccagggct cggcgatcag cttgacgcga ttgagcaccg gatcctgccg tacgacgctg
     2821 aaaaagccgc cgagctgctc gaccgcgcgt ccctcgcgcg ccagggccgg cgccaggtcg
     2881 aagcgaaagc cgtccacgtg catctcctcg acccagtagc gcagcgaatc catgaccatc
     2941 tgcagcacgc gcgggtgctc gaggttgacg gtgttgccgc agccggtgaa atcgaggtaa
     3001 tggcggcgct cgtcctccat caggcggtag tacgaggcat tgtcgacgcc gcgaaaggcg
     3061 agcgtcggac cgaggtggtt gccctcgcaa gtgtggttgt agaccacgtc gatgatcact
     3121 tcgatgcccg ccgagtgcag gcgcttcacc atggtcttga attccttcga gctgccggag
     3181 gccgaatagc gcggctccgg cgcgaagaag ccgagcgtgt tgtagcccca gtagttgcgc
     3241 agcccttgct cgacgagacg gcggtcgtcg atgaaggcgt gcaccggcat cagctccacc
     3301 gtggtcacgc ccaggcgctt gaggtactcg atcaccggcg ccgagcacag cccggcatag
     3361 gtgccgcgct gcggctccgg cacttcgggg tgccgcttgg tgaagccgcg cacgtgcagc
     3421 tcgtagatca ccatgtcgtg ccacggcacg ctcgggcgcc ggtcgtcgcc ccaggtgaac
     3481 gccgtgtcga ccacgcggca cttgggcatg aacgcggcgc tgtcgcgctc gtcggccttc
     3541 aggtccgcct cggcggcgcc gatggtgtag ccgtagagcg catcgttcca gcgcacctcg
     3601 ccgaccagct cgcgcgcata tgggtcaatc agcagcttgt agccattgaa gcggtggcca
     3661 tctttgggcc ggtacgggcc gtgcacgcgg tagccgtaca cctggccggg ccgcgcctcg
     3721 ggcaggtagc agtgccacac gttgtcggtg cgctcccgga tctcgattct ctgcagctcg
     3781 cgcgtgccgg agggctcgaa caggcacaac tcgactttct cggcgtgctc ggagaaaagc
     3841 gcgaagttga cgccctcgcc atcccaggtc gcgcccttgg gatacggcgc gccgcgccat
     3901 accgcgccga gcggcggcgc ctcgctcacg gttgcgcgcc gagcgtcatt cgccgcgtcc
     3961 ccgcggccac cagcgctcga gcaggcccgc ggcccatttc tgcccgccga tgccgaaggc
     4021 gagcgccagg ccgagcacca cgccggcgag gaggatgagg aagctgtggc gcacgacgtc
     4081 gccgccgatg ttcacctggt cgagcgccat cagcaccacg aaggcgatga tggcgtactg
     4141 cgccagcttg ccgagcagat ccccgtcctt gatgccgacg ttgccgcagt acgtggcgac
     4201 cgtgttgccg acgaacatgg cgaagtagcc gccgaaggca aacagcagca gcgccacgat
     4261 caccttgggc acgaacagca ccacctggcc gagcaggtcg gtgatgtagg tgagccccag
     4321 gccgttgaag gcgatgacca gggcgatgaa gatcaccagc cagtacacca gcacggcgaa
     4381 gatgtccgtg gtgtcgctct ggatgccgcc ctgcttcaga aagccgtcca tgccggcgcg
     4441 ctcggtgagc acgttgaagt tgatggcgcg cagggccttg gccaccgtga agcgcgccag
     4501 gcgcgccagc agccagccga cgatcagcac cacgagcgcg agaccgagct tgggcaggaa
     4561 tacgccgatc tgcgtcagca ccgcccgcat cggctccaga aatacgttga tatccatggg
     4621 gtcctccagt cctggctagc gcgcgagcgc aagtatgccc tgcagcggga tgccgaccca
     4681 gcccgggcgg ttgccgagct cgtagcgcag ctcgtaaagt gctttttcca gctcgaacag
     4741 ctcgagcagc tcgtgcatgt cgtggaacga ggcgtacagg ccgctcgcgc gcgcggcctg
     4801 ctcgtaggcg ccgaggaacg tgcgccgcac gcgcgcctcc cagtcctcgg cgaagggcgc
     4861 gaggcgctca tagtcgttgt ggccttcgag cgcctggcgc agggcggtcc agcgcgcgta
     4921 gttgaacgag cgcagcatgc cggcgacgtc cttcagcggc gagtgcttgg cgcggcgctc
     4981 ctcgatcgaa cgcgccggct cgccctcgaa atcggtgatc acgaaatcga gctgttgcag
     5041 cagcacctgg ccgagatggt agtcgccgtg aaagcgcgtt ttctgcgtcg cgcccttggg
     5101 caggcggtag ccctcgatgc gccgcagcag ccgctcgcgg ctttccacca gctcggcggc
     5161 gtcgtcgcgc gccggcgcgg tgagctggcc gcggcgcgcg gcgagcatgt cgagcgtctt
     5221 cgcggcctcg gtgcggatgc ccgccttcca ttgcgaaaaa tcgcgatcgg tcacgggctc
     5281 cgggtcgaac gcggcgtcgc cggtgcgcgt ggcgagcgcg cagtgcagct cgccggtgcg
     5341 cgtgccgagg gtctgcatga gcggcaggta gatgccgtga tcgccgcccg cggcgccact
     5401 cgcgcgctgg ttctcgagga agcgctcgag gtactccacc gtgtggttcc agccgtcgcc
     5461 ctggttgggg acataggcct gcagcagcgc gagcgtcatc ggcgtgccgt cggccgccgt
     5521 gtactccacg gcgccggcga ccggcacgca gttggcgtag cgcactttct cggtgaggaa
     5581 acgcccgacc tcgaactcgg ggttcacgcc agggcgtatg cgccgatagc ccttcaggaa
     5641 catgcgctcg cccagcgtca ccacggtgtt gctgctttgc gccttgggcc ggcccacggg
     5701 cagcaggttg acgtcttcgc tgcagacctc ggcgaaggcc gcagtcggcg tgaagcgcag
     5761 cttgccctgt gcgccggcga gctcgcgtcc ggcgccgatc gcctcgacca ccgcgcggca
     5821 gaaggcttcg tcggcaaaag cgtcggccat gatgccgacg ctcgcctgct ggcgcacctt
     5881 ggcaacggcg gcgccgccga gggccttcac gcgctcctcc tcgcggtctt cccaggcgag
     5941 cgtgagcggg gcaaagtagc gcgcggcttc ggcgccgcct tcgacctcga gcagcggcag
     6001 catgtaggtc gcgcggcccg cttcctccca cagcgcccag tcgcccagcc gcacgcgcgc
     6061 gcccgtgtcg cccttggcgg cataccagcg ctgcgtctcg agccagcccg gcagcacctg
     6121 ggtctcgaag tgctcgcgcg tcttcaccgc catgttgatg cgccagggca ccacgcggtc
     6181 gcggaaaaag ctcgtccagc cgtcgaacat caccagcatg ggcaggtcct cccgcgccag
     6241 tacttcggcg tgccacacgg gcgccgcggc gtccgtggcg aggcgaaacc agtagaaacc
     6301 gcagcccggc agggtgagca ggtagggcag ctcgccgatc ggcggaaaca ccgtgcgccc
     6361 catcagctcg accggcacgc ggcccttgaa gcgccgcagg tcgagctcca ccggctgcgc
     6421 cgtgcgcccg acgttggcga cgcacaggat cacctcgtcg ccgtactccc gcacataggc
     6481 gagcaccttg cggttgccgg cgcgcaggaa ctgcagcttg ccgcggccga aggcgtggct
     6541 cgttttcctg acggcgagca ggcgccgcat ccagttgagc agcgaattcg gctcgcgcga
     6601 ttgcgcctcg acgttcaccg cctcgaagcc gtagatcggg tccatgatcg gcggcaggta
     6661 caggcgctgc gggtcggcgc gcgagaagcc ggcgttgcgg tccggcgtcc attgcatcgg
     6721 cgttctgacc ccgttgcggt cgccgaggta gatgttgtcg cccatgccga tctcgtcgcc
     6781 gtagtagatg atcggcgagc ccggcatggt gagcaggagg ctgttcatca gcttgatgcg
     6841 gtcgatgtcg ttctcgagca gcggcgccag gcgccggcgg atgccgaggt tgatgcgcgc
     6901 ccgcgtgtcc gccgcgtacg tgcggtacat gtagtcgcgc tccttgctgg tcaccatctc
     6961 gagcgtcagc tcgtcgtgat tgcgcaggaa gatcgcccac tggcagctct cggggatctc
     7021 gggcgtctgc tgcatgatct ccaccaccgg gtggcggtct tcctgcgcga tcgccatgta
     7081 catgcgcggc atcagcggga agtggtaggc catctggcac tcgtcggcct cgccgaaata
     7141 ctcgcgcacg tcctcgggcc actggttggc ctcggcgagc agcagcttgc ccgggtagcg
     7201 cgcatcgagc gcggcgcgga tcctcttgat cacggcatgc gtctcgggca ggttctcgtt
     7261 cgaggtgcct tcgcgctcga cgaggtacgg gatcgcgtcc aggcgaaaac cgtccacgcc
     7321 cagctcgcac cagaacgcca tcacttccag caccgcctcc agcaccttcg ggttgtcgaa
     7381 gttgaggtcg ggctggtggc tgaagaagcg gtgccagaaa tactgcttgg ccaccgggtc
     7441 ccaggtccag ttcgaggtct cggtgtcggt gaagatgatg cgcgtgccct tgtacttctg
     7501 gtcgtcgtcg ctccagacgt agaagtcgcg ctcgggcgag cccttgggcg cgcgccgcgc
     7561 cgcctggaac cagggatgcg cgtccgaggt gtggttgacg atcagctcgg tgatcacgcg
     7621 caggttgcgc ttgtgcgcct cctcgacgaa gacgcggaaa tcctcggcgt tgccgtattg
     7681 cgggtgcacc tcgcggtagt cggcgacgtc gtagccgtcg tccttcagcg gcgaggggta
     7741 gaacggcagc agccagatgg tgttgacgcc gaggtcgcgc acgtagtcga gcttctcggt
     7801 caggccgcgg aagtccccca ggccgtcgcc gttggaatcg tagaaggcct tgatgtgcag
     7861 ctggtagacg accgcgtcct tgtaccagtg cgggtcggcg gcggcgggcg cggcggcctg
     7921 gccggcagct gccggcgcgc tcgtcgtcgt ggtcgtgggg gcgttcacgg gttcacagga
     7981 agtagtcgaa atcctgctcc cggtgcaggc gtcggcgcac gcggaacacg tgcgcgggcg
     8041 agcgctgcgg gtcgagctgc acgaagttgc gcggcccgtt ccacatgtag cgcgcgcccg
     8101 agagcaggtc gtgcatctgg taggggcgat cgggctcgat gccgagcgcg ccgaggtcga
     8161 gctcgaccca gccgccgtgc gtgtggtgcg ggctgaggtt gaccacgcag acgatcaggt
     8221 tcgacccatc cgccgcctgc ttgctgtagg cgagcagctg atcgttgtcg gtgttgcaaa
     8281 agcgcaggcc ggcgtcggac tgcagcgccg gattctcgcg ccggatgcgg ttgaacgccg
     8341 cgatgtaggc gcgcaggctg tcggcgcgct ccaggtccca gtggcgcagc tggtatttct
     8401 ccgagtgcag gtactcctcg ctgccgggct cgcgcggcag gtgctccatc agctcgtaag
     8461 ccggaccgta gatgccgtag ctcgccgaga gcgtcgcggc gagcgcgagg cgctgcatga
     8521 acaccgcgcg cccgcccacc tgcagcgctt cggtcaggat gtcgggggtg ttcggccaga
     8581 cgttggggcg gaagtactcc ttgcccggcc cctgcgcgag ctccgtgaag tactcggtca
     8641 gctcctgctt ggtgttgcgc cacgcgaagt aggtgtagga ctgcgtatag ccgagcttgg
     8701 cgaggcggtg catgaccttg ggccgcgtga aggcctcggc aaggaacagc acgtcgggat
     8761 gcgcgcgccg tatttcggcg atggcccact cccagaacgg gaacgccttg gtgtgcgggt
     8821 tgtcgacgcg gaagatcttc acgccttcgc cgatccagaa gtcgaacacg ctcttcagct
     8881 cctgccacag cgccttccag tccgcgcact cgaagtcgaa ggggtagatg tcctggtact
     8941 tcttcggcgg gttctcggcg tactgcaccg agccgtcggg gcgcttgcgg aaccagtccg
     9001 gatgctcctt cacgtacggg tggtccggcg cgcactggaa ggcgatatcg agggcgatct
     9061 cgatgccgtg cttcgccgcc gccttgacca ggctgcggaa atcctccggc gtgccgagcg
     9121 ccggcaggat ggacttgtgc ccgccttcct tcgcgccgat ggcccagggg ctgccgaggt
     9181 cgtccgcgcc cgccgtcacc gcgttgttct tgcccttccg gcgctcgcgg ccgaccgggt
     9241 ggatcggcgg caggtagagc acgtcgaagc cgagctcggc gacgtacgcg agtcgcttct
     9301 ccacgtcctt gaaggtgccg tgcttgccgg gctcggcgga cgccgagcgc gggaacagct
     9361 cgtaccaggt ggagtagcgc gcgcgctcgc gatccacgac gagcggcatg tcgacgcccc
     9421 agcgcgcctc gagcgagcgg tcgggatagc gcgcggcgag cgccgccatc acttcgtcga
     9481 gcgccgtggc gcggatctcg accggcttct tcgcggcgcg cagccgcgcc gcccagtcct
     9541 tgagctgctg cgcgtcggcg cccgcatttt cgtgcttggc gcgcgcggca gcctcctcgg
     9601 caaggtaggc gccggcgagc gcggcgagcg cctggtcggc aggatcctcg cgccgggcga
     9661 agtcgtggcg ccaggtggcg aaggcgtcca cccaggcggc cgcgctgtag cggtagcgcc
     9721 cgatctcctt gggcgtgaac tgcgcgcgcc agacgtcgtt gccgagcggc ttcatctcga
     9781 cttcctgcca gtcgcgcgcg tcctcggcgc gccacagcag ccgcgcgcgc aacacatcgt
     9841 ggccgtcggc gaagcagtgc gcctcgactt cgagcgcctc gcccgcgacg cgcttgaccg
     9901 cgaagcgccc gccgtccacg gcggggcgca ccgcgtcgat taccgcacgc acgcgtccgt
     9961 cggatgcgcc ggtcgggctc atgtgtcgct cctcaggtaa acgctcgaca gcggcggcag
    10021 cgtcagcgtg agtgaatgcg gccgcccgtg cgcggccagc ggcgccgcct gcaccgcgcc
    10081 gaagttgccc cagcccgcgc cgccgtactc cttggcgtcg ctgttcacga tctcgcgcca
    10141 ggtgccgccg cgcggcacgc cgagcacgta gttctcgcgc ggcagcggcg tcaggttgca
    10201 caccacggcc accagaggtc cttttccgcg cggcttgcgc aaaaacgaga tggcgctgtt
    10261 ctgggcgtca ttggcgtcga tccactcgaa accctcgtgc gagaagtcta cctgccacag
    10321 cgcgggctcg gcgcggtagg cgcggtttag gtcgcgcacc cagcgctgca gtcccgcgtg
    10381 ctcgggccgc tcgcataccc accattccag ctcgccgtcg tgcgtccact cgcgccgctg
    10441 gccgaactcg ccgcccatga acagcagctt cttgcccggg tggccccaca tgtagccgta
    10501 gagcgcgcgc aggttggcga actgctgcca ggcgtcgccc ggcatcttgc cgatgagcga
    10561 gcccttgccg tgcaccacct cgtcgtgcga gagcggcagc gtgaagttct cgttgaaggc
    10621 gtagatcagg gagaacgtga ggcgcccgtg gtggtacttg cggtggatcg ggtcttcctt
    10681 gaagtaggcg agcgtgtcgt gcatccagcc catgttccac ttcaggccga agcccaggcc
    10741 gccgaggtag gtcggccgcg acaccatcgg ccaggcggtc gattcctcgg ccgcggtgaa
    10801 gcaatccgga tggtcgcggt agacgccctc gttcagcgtg cgcaggaacg agatcgcctc
    10861 caggttctcc cggccgccgt acttgttcgg tatccactcg ccgtgcttgc gcgcgtagtc
    10921 gaggtacagc atcgaggcca ccgcgtcgac gcgaatgccg tcgatgtggt acttgtcgag
    10981 ccagaacagc gccgaggaca gcaggaacga gcgcacctcg ttgcggccgt agttgaagat
    11041 gctcgagttc cactcggggt gaaagccctg gcgcgggtcg gcgtgctcgt agagatgcgt
    11101 gccgtcgaaa aagccgaggc cgtgcgcgtc ggcgggaaaa tgcgacggca cccagtcgag
    11161 gatcacgccg atgcccgcct ggtgcaggtg atcgacgaga tacatgaaat cctggggcgt
    11221 gccgtagcgc gcggtgggcg cgaagtagcc cgtcgtctgg tagccccacg agccatagaa
    11281 cgggtgctcc gtgagcggca tcagctccac gtgcgtgaag ccggcctcct tcacgtacgc
    11341 ggcgagctcg tgcgccagct cccggtagcc gaggaaggcg ccgttcttgc gcttccacga
    11401 gccggcgtgc acttcgtaca cggacatcgg cgcttcgagc gcgttcttcg gcccgcgcgt
    11461 cttcatccac tcggcgtcgc cccagtcgta ttcgagcgtc cacacgcgcg agccggtggc
    11521 cggcggcagc tcggccgcga cggcgaacgg atcggccttc tccgagacat ggccgccgac
    11581 gtgcgattcg atgcggaact tgtaggcttg gccccgttcg gcgccgcgta cgctgccttc
    11641 ccagatgccg gagtggtcgc cgcgcggcgc gagcatgtcg gcgcccgcac gccagccgtt
    11701 ccagtcgccg atcaccgaca cggcgcgagc attgggcgcc cacacgccga agtgcgcgcc
    11761 cttcttcttg ccgtcgggat ggcagcccag gcgctcgtaa aggcgcgcgt gcgtgccttc
    11821 gcggaaaaga taagcgtcat gctcggtcaa caaggtgccg gcctcccgtg tttggggtcg
    11881 tggtcgctgt cggaccggag gattcgctgc gcgggataga cgcgcccggc ggctgcttga
    11941 tggagcgcgc ttcgcgattt gcattgcgct gcggcatttt atcacggcgt cgccctgcga
    12001 acaacctgcg agcgcgatca cgcgcgcacg atcgaaactt tgcgtcgcgc gtgcgtcggc
    12061 gttatgttct tatgtcctgc aacctcattc actgcagcga gcggaggagc cattgcgaaa
    12121 cgaagatagg tcggcggtga ctcgatgaag ctgtgggagc agttgctgag cgataacgcg
    12181 ccgggcgcgt ggctgctcgc cggcgcgatc gtgttcgcgg tgctcgccgc agtgtggctg
    12241 gcgcgcgcga tcgttgcgcg ggtggtcacg cgcatcgcgc gccgcaccgc tttgcagctg
    12301 gacgatgcgc tgatcgccgc ggtgcgcgcc acgaagctgt ggctgctgac gccgctcgcg
    12361 ctctacgccg gcgcctccgc gctcgagatc ccgccgcgcc tcgaccggct gctcgagcac
    12421 ctcgccatgg tgggcctgat cgcgcagttc gcggtatgga cgaatcgctg ggtgctgcga
    12481 tggctggcgc tgcgcgcgga cgcggcgcgc gagaccgacc cggccgccac caccaccgtc
    12541 aacctgctcg gcttcgtcgc gcgcgtggtg gtgtgggcgc tggcgctgct catcgcgctc
    12601 gatcagctcg gcttcaacgt cacggcgctg gtggcgggcc tgggcatcgg cggcatcgcg
    12661 gtggcgcttg cggtgcagaa catcctcggc gacctgttcg cgtcgatggc catcgtgctc
    12721 gacaagccgt tccaggtggg cgacttcatc atcgtcggcg acaagcccgg cacggtggag
    12781 aaggtggggc tgaagacgac gcgcgtgaag agcctgtggg gcgagcaata cgtgttcgcc
    12841 aacgcgcagc tgctcaacag ccagatccgc aactacaaga agatgcggga gcggcgcatc
    12901 gccttcggca tcggcgtcac ctacgagacg ccggcggaga agctgcgcgc gctgcccggc
    12961 tggctcaagg cggcggtcga agcgcagccc gacctgcgct tcgaccgcgc gcatttcaag
    13021 tcctacggcg atttctcgct cgatttcgag atcgtgtact acgtgctcag tccggactac
    13081 aacctgtaca tggaccggca gcaggcgatc aacctcgcca tcttcgacaa gctggcgcag
    13141 gaaggcgtgc atttcgccta tccgacgcgt acgctctatc tgcggccgga accggccgca
    13201 ggccgctgag ggggcgggca tgaagggcgc gggggcgggc gcgggccgcg agcggctgat
    13261 cgtcgtctcg aatcgcctgc ctttcacatt caagaaggac ggcgagacgt ggcgctcgga
    13321 gccgggcggc ggcggactgg tcaccgcgct gctgccggtg ctgcgcgacc gcggcggcat
    13381 gtggatcggc tggccgggaa ccaccgggga ccccaaggag cttgatgcgg cgctcgccgc
    13441 cgccggcgcc aacgccggct acacgctcgc cggcgtgccg ctcaccgccg acgagatgga
    13501 caagttctac ctcggcttct ccaacgagat catctggccg ctgttccacg acctgcagtc
    13561 gctgtgcaat ttcgacccgg cgtactggcg cacctactgc gatgtgaacg cgaaatacgc
    13621 cgacgtggtg aaggcgcaca ctgcgccggg cgacttcatc tggatccacg actaccacct
    13681 gatgaacgtg gccgccgagc tgcggcgccg cggcgtgcgc tcgcgccagg gattcttcct
    13741 gcacatcccg ttcccgtcgc ccgacctgtt catgaagctg ccctggcgac tgtcgctgct
    13801 gcaggcgctg ctggaattcg atctgatcgg cttccagacc gcgctcgacc ggcgcaactt
    13861 cgtgcagtgc gcgcgcatgc tggtgaagga cgtgcagatc gagggccgcg ggcaggttct
    13921 ggaggcgcgc gttggcaagc gcacggtgag aatcggcgcg tttcccatct ccatcgacta
    13981 cggcgcgttc cagcgccgcg ccgccgcgcc ggaggtcacc gccaaggcgc aggagctgca
    14041 ccggctgctg cccgacagga agctgattct cggcatcgac cggctcgact acaccaaggg
    14101 catcccgctg cggctgaagg cgttccaaaa cctgctggag cgctatcccg agctgcgcga
    14161 gcacgtgtcc ttcctccagg tggtcgtgcc cagccgcgag gacattccgg aatatcaccg
    14221 gctgcgcacg gagatcgagc aactggtggg gcacatcaac ggccgcttcg cgcgcccggg
    14281 cggctgggtg ccgatctggt acgagtaccg caacctgtcg cgcctggagc tcctcgccta
    14341 ttaccgcgcc gcgcacatcg cgctgatcac gccgctcaag gacggcatga acctggtggc
    14401 gaaggagtac tgcacgtgca gcatcgagga ggactgcgtg ctgatcctgt cggagttcgc
    14461 cggcgcggcc tcacagctct accgcggcgc gctgctggtc aatccctacg acatcgaggg
    14521 cgtcgccgac accatccagc gcgcgcacgg catgcacccc gaggagcgcc acgcgcgcat
    14581 gcggcgcatg cgaaattcga tccgcgagca cgacgtgttc tggtgggtgg actcgttcct
    14641 gcgcgcggcg atcacccggg aactgagctc cttcccgcag cccgaagggc acccggagga
    14701 agacgcgacc gagtaccagc tgccgttcta gctcaggtga ggccgtcgaa cggctgcacc
    14761 tgcaccggct cgcccgcctg cacgcgcccg cgctcgtgct cgagcacgat gacgcagttc
    14821 gcttccgaca tggagcgcag cacgcccgag ccctgctggc cggtggtgcg caccttgagc
    14881 tcggcgccct cccggtagag catcccgcgc tggtattcgg tgcggcccgc gaccttgcgc
    14941 agcggatcgg ccgcacgcgc ctcgagcagg ggcagcgcgg cgccgctgcg tccggcgagc
    15001 tgccacagcg cctcgcgcac gaactggtag aaggtcacca tcaccgccac cgggttgccc
    15061 ggcaggccga acagaaacgc gttgccgatg cgcccgaacg ccatcggccg ccccgggcgc
    15121 atggcgatgc gccagaacag cgcctcgccg agcttggcca tcatcggctt gacgaaatcg
    15181 gcttcgccca ccgatacccc gccggtggtg atgatggcgt cggcgttcga tgcggcgtcg
    15241 cgcaacgcgg attcgagctc gcgcgggtcg tcgcgcacca cgccgaggtc gcgcgcctcg
    15301 acgcccaggc gcgcgagcat gccgtgcagc gtgtagcgat tggagtcgta cacctcgcct
    15361 tgcctgagcg cgctgccgac ggacgcgagc tcgtcgccgg tggaaaagaa cgccacgcgc
    15421 acgcgccggc gcaccgccac ctcgccgatg ccgagagagg cgatgagccc gagctcggcg
    15481 ggcgcgagca ggcgccccgg cgagagcacc ggcacgcctt tcttcagatc ctcgccggcg
    15541 tagcgcacgt tctgcttggc tttctggccg gccgggatcg tcactttcgt gccgtgcacc
    15601 ttcacgactt cctgcaccac caccgtgtcg gtgcccgcgg gcatcaccgc gccggtggtg
    15661 acgcgcacgc attgccccgc gcccacggtg ccggcatagg ccttgccggc gagcgcggcg
    15721 ccgatctcct cgagcaccgt cgcgccggcg ccgagatcgg cgtggcgcac ggcgtagccg
    15781 tccatcgccg tgttgtcgtg gcccggcacg tcgatcgcgg gaacgatgtc ctcggcgagc
    15841 acgcgcccga gcgccgcgcg caccggcgcg cgttcgcgct ccttgaccgg cgcgaggcag
    15901 gcgcggatcg cggcgcgcgc cttgtccacg tgcagcgcgt cggggtcgta gccctcgatc
    15961 gcctgcgcga tctcgatgat gcgcttcacg cctgtcccag ctcctcgcgc gtgttgatgt
    16021 tgcgaaacgc gtcggcctcg tcgtcgaacg gcacctcgac gaccttcagg ctcgcgtacc
    16081 aggcgtcgat cttgcggccg cccgcggcga gaaacgcgcc gaggtgcgcg tgcaccgagc
    16141 ggcgcacgag cgagaacacc gggtgcggct ggtctgccgt cttcgccacg gcgaggtcgt
    16201 gcgcgcccag tgccgcgcgc agccgcgcca ccaggtcgag cggcaggaac ggcgagtcgc
    16261 acggcaccgt ggcggcgagg gggtggctga tggcggcgag gcccgcatgc agcccggcaa
    16321 gcgggccggc gaagccgccg atcgcatcgg gcaccacgcg gtagccgaag gcgccgtagg
    16381 cgtcgaggtt ctggttggcg ttgatcacga tttcgccggc ctgcggcgcg aggcgctcca
    16441 gcacccaggc caccatcggc ttgccgcgaa ggaccaccag ccccttgtcc acgccgccca
    16501 tgcgccgccc ctggccgccg gcgagcacga ttgctgaaac agcgtccatg ccgcggattc
    16561 tacgaggtaa gcttggcgcg atgaacgacc ggcgctggct ggtgcccttc ctcaacctcg
    16621 ggcacctgct cgatcacctc gcgatgctcg tctttccgac ggcggtgctc gcgctgggcg
    16681 cggcatggaa ccgcccgtat tcggagctgc tgccgctcgc gctgggcggc ttcatcgcct
    16741 tcggcgcctt tgcgattccc gccggctggc tcgccgacca ctggagccgc taccgggtgc
    16801 tggcgatctt cttcttcggc atcggcgcat cgctgttcct caccggcttg gcgcgcgagc
    16861 cctggcacat cgcggtcggg ctgacgctgg tgggcgcgtt cgccgccatc taccatccgg
    16921 tcggcatcgc catgctggtg gcttcgccag agaagatggg gcgcgtgctc ggctggaacg
    16981 ggctgtgggg caacctcggc ctcgcctttg cggcgctgct caccggcgcg ctggtggacc
    17041 ttgccggctg gcgcgcggca ttcttcgtgc cgggcctcat ctgcatcgcg gcgggcgtgg
    17101 gcttcctcgc cgtggtgaag gatccgggcc gggtgaagaa gtcctcgaaa tcgatcggcc
    17161 tgcacgtcga cgggcgcacc atggcgcgca tctttaccgt gctgctcgtc gccacggcct
    17221 gcggcggcat catcttcaat tccaccacgg tgtcgatgcc gaaggtgttc gacgagcgcc
    17281 tgcgcgcgct gacgcagacc aacttcggca tcggcgcgct ggtggcggcc gtgtataccg
    17341 tggcggcctt cgctcaggtg gcgatgggcg cgctcatcga ccgcttcgag ctgcgccggc
    17401 tcatgatcgg cgtggcgctc gcgcagatcc cgctgctcgc gctcgccgcc aacctgcagg
    17461 gctgggccat gctcggcgcg gcggtgctca tgatgctcgc ggtattcggt caaattccgc
    17521 tcaatgacgc catcgtcgga agatactgcg ccgacgagtt ccgcg
//
LOCUS       DBNHKE010000109         3425 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1075996, whole genome shotgun sequence.
ACCESSION   DBNHKE010000109 DBNHKE010000000
VERSION     DBNHKE010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1075996"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16O9_06775"
     CDS             <1..513
                     /locus_tag="AC16O9_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR6516356.1"
                     /translation="YDLVDLEYSDEAPLRARAGHPPRLPDNPLAEPEVRTAERHEIVF
                     TGGMMGDMRGLPRGAAWAVNGVANGCGEGGVPFEPLLVLRKGRSYVLNLVNDTLWHHP
                     IHLHGHSFRVVSRDGRPTRYREWLDTVMLAPRERAEIAFVADNPGDWMFHWHVLEHQA
                     SGMMSCIRVG"
     gene            516..956
                     /locus_tag="AC16O9_06780"
     CDS             516..956
                     /locus_tag="AC16O9_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6516357.1"
                     /translation="MMLVAMLAGCEPAATGYDDARADPRDASRVALGERVYAQHCASC
                     HGAKLEGQPNWRSRLPNGRLPAPPHDESGHTWHHPDRLLFAITKNGLVPPYVPKGYAS
                     DMPAYAGSLTDDEIWAALAFIKSRWTSREVREARAQMSAGASVR"
     gene            1019..1750
                     /locus_tag="AC16O9_06785"
     CDS             1019..1750
                     /locus_tag="AC16O9_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR6516358.1"
                     /translation="MKQMAVIMALLAVASAAVADEAAVRRLIQSRAGDQAKIEQILRL
                     PGSSLYEVTLRGPNGPQLFYVDDKARVIVVGQAIDGRSGLNLTEERLRKLNAIDWRTL
                     PFADAITTTRGSGRRKVAVFSDPNCPYCKRFEKDLAKLDDIAVHIFVYPVIRPDSVRL
                     TKSVWCSRDRAKAWNDLMLRDIEPTAAPDCDTPVEKLVALGRSLGATSTPTWFVETGE
                     RYSGAIPLEDTRRILDDAARLKGAK"
     gene            1854..2453
                     /locus_tag="AC16O9_06790"
     CDS             1854..2453
                     /locus_tag="AC16O9_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6516359.1"
                     /translation="MTAGRPPLRVLLASVGAAALVAGGVGTYLSLERSGEQSGAPTVT
                     PAPSETSIIRLHAAPRALPPIAFQDGKGRPVTLADFRGKAVLLNVWATWCAPCREEMP
                     SLDRLQKALGGPAFEVVAVSIDTGGAAVVERFYKEVGIGALAIYVDPTMRAAGELRTP
                     GVPTTLLIDAEGRELGRHAGPASWDDAEVLKLVARHLPR"
     gene            2453..3193
                     /locus_tag="AC16O9_06795"
     CDS             2453..3193
                     /locus_tag="AC16O9_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR6516360.1"
                     /translation="MEAGTIGLAAAFAAGMISFLSPCVLPLVPAYVSYVAGQSNSALE
                     RRRDAHQRLAALALSSVFVLGFGVVFVALGAGASALGSLLLRFRYEANLIGGAIVIVF
                     GVLMIGMSRGMPWLQREWRFHPNLPGGRPLAAFVLGLAFGFGWTPCIGPVLGAILTLG
                     ATQAPGAGAVGLLAVYAAGLGVPFLLVALFTRELAARMRALRRVGWILQIAAGAVLVA
                     MGIAMMTGYLSKFGFWLLETFPALGRIG"
BASE COUNT          501 a         1164 c         1215 g          545 t
ORIGIN      
        1 tacgatctcg tggatctcga atacagcgac gaggcgccac tgcgcgcgcg cgccggccac
       61 ccgccgcgcc ttccggacaa cccgctcgca gagccggagg tgcgcaccgc cgagcgccac
      121 gagatcgtgt ttaccggcgg catgatgggc gacatgcgcg ggctgccgcg gggcgcggcc
      181 tgggcggtga acggcgtggc gaacgggtgt ggcgagggcg gcgtgccgtt cgagccgctg
      241 ctcgtcttgc gcaaagggcg cagctatgtg ctaaatctcg tcaacgacac gctctggcac
      301 cacccgatcc atctgcacgg gcattcgttc cgcgttgtat cgcgtgatgg ccgcccgacg
      361 cggtaccgcg agtggctcga caccgtgatg ctcgcgccgc gcgagcgcgc agagatcgcg
      421 ttcgtcgccg acaaccccgg cgactggatg ttccactggc acgtgctcga gcaccaggcg
      481 agcgggatga tgtcgtgcat ccgcgtcggc tgagcatgat gctagtcgcg atgctcgccg
      541 gctgcgaacc ggcggccacc ggctacgacg acgcgcgcgc cgatccgcgc gacgcgtcgc
      601 gcgtcgcgct gggcgagcgc gtgtatgcgc agcactgtgc gtcctgccac ggcgcaaagc
      661 tcgagggaca gccgaactgg cgcagccggc tgccaaacgg ccgcctgccc gcgccgccgc
      721 atgacgagag cgggcacacc tggcaccatc cggatcgcct gcttttcgcg atcacgaaga
      781 acggcctcgt gccgccgtat gtgcccaagg gctacgcgtc ggacatgccg gcgtacgcgg
      841 gcagcctcac ggacgacgaa atctgggccg cgctcgcctt catcaagtcg cgctggactt
      901 cccgtgaggt acgcgaggct cgcgcgcaga tgtcggcggg cgcgtcggtg cgatgaaaca
      961 tcgcgcgtga gcagggggcc gacgcgggat tgaggaccgg aacgaggagc acgctgggat
     1021 gaagcaaatg gcggtgatca tggctctgct cgccgtggcg agcgccgcag ttgcggacga
     1081 ggcggcggtg cggcggctga tccagtcgcg tgccggcgat caggcgaaga tcgagcagat
     1141 cctgcgcctt ccgggcagca gcctgtacga ggtgacgctg cgcggcccga acggaccgca
     1201 gctcttctac gtggacgaca aagcgcgcgt catcgtggtc ggccaggcga tcgacgggcg
     1261 cagcggcctc aatctcaccg aggagcgcct gcgcaagctg aacgccatcg actggcgcac
     1321 gctgccgttc gccgacgcca tcacgaccac gcgcggcagc ggccggcgca aggtggcggt
     1381 gttttccgat ccgaattgcc cctactgcaa gcgcttcgag aaggacctcg ccaagctcga
     1441 cgacatcgcg gtgcacatct ttgtctaccc ggtgatccgg cccgactcgg tgcggctcac
     1501 caagtcggtg tggtgctcgc gcgaccgggc gaaggcgtgg aacgacctca tgctgcgcga
     1561 catcgagccc acggcagccc ccgattgcga cacaccggtc gagaagctgg ttgcgctcgg
     1621 gcgcagcctc ggcgcgacct cgacgcccac gtggttcgtc gagaccggcg agcgctattc
     1681 gggggcgatc ccgctcgagg acacccggcg tattcttgac gacgcagcac gcctgaaagg
     1741 agcgaaatga cgcgaacgat ccgattcttc cttgccgcct tccggcgtaa cgtctatctg
     1801 agccccgacc gtgggcgcac gtggcgccag atcgccgcgg acggcaagtg gcaatgacgg
     1861 ccggccggcc gccgctgcgt gtgctcctcg cgtcggtcgg cgcggcggcg ctcgtcgccg
     1921 gcggggtcgg gacgtacctg tcgctcgagc ggtctggaga gcagtcgggc gcacccacgg
     1981 tgacgccggc cccgtcggaa acgtcgatca tccgtctgca cgcggcgccg cgcgcgctgc
     2041 ccccgatcgc gttccaggac ggcaagggtc gcccggtcac ccttgccgac tttcgcggca
     2101 aggccgtgct gctcaacgtg tgggcgacgt ggtgtgcgcc gtgccgcgag gaaatgccct
     2161 cgctcgaccg cttgcagaaa gcgctcggcg ggcccgcttt cgaagtcgtg gccgtgtcga
     2221 tcgacaccgg cggcgccgcg gtggtggagc gcttctataa agaagtcggt atcggcgcgc
     2281 tcgcgatcta cgtcgatccc acgatgcgcg cggctggcga gctgcgaacg ccgggcgtgc
     2341 ccacgacgct gctgatcgac gccgaggggc gcgagctcgg ccgccacgcc ggtcccgcga
     2401 gctgggacga tgccgaggtg ctgaagctcg tcgcccggca tctgccgcgc tgatggaagc
     2461 cggcacgatc gggcttgccg cggctttcgc cgcgggcatg atctcgtttc tctcgccgtg
     2521 cgtgctgccg ctcgtgccgg cctacgtctc ctatgtcgcg ggccagtcga actcggcgct
     2581 cgagcgccgc cgcgacgcgc accagcggct cgccgcgctc gccctgagca gcgtgttcgt
     2641 cctcggcttc ggcgtcgtgt tcgtcgcgct cggcgcgggc gcaagcgcgc tcggcagcct
     2701 gctgctgcgc ttccgatacg aggcgaacct catcggcggc gcgatcgtga tcgttttcgg
     2761 cgtcctgatg atcggtatgt cgcgcggcat gccctggctg cagcgcgaat ggcgttttca
     2821 cccgaacctg cccggcgggc ggccgctcgc cgcgttcgtg ctcgggctcg ccttcggctt
     2881 tggctggacg ccgtgcatcg gcccggtgct cggcgccatc ctgacgctcg gcgcgacaca
     2941 ggccccgggc gcgggcgcgg tcgggttgct cgcggtgtac gccgcgggcc tcggcgtgcc
     3001 gttcctgctc gtggcgctgt tcacgcgaga gctcgcggcg cgcatgcgcg cgctgcgccg
     3061 cgtgggctgg atcctgcaga tcgccgcggg cgccgtgctc gtcgccatgg ggattgcgat
     3121 gatgacaggg tacctgtcga agttcgggtt ctggctgctg gagacgtttc cggcgcttgg
     3181 gcggatcggg tgagtgcaac ggggcagcat gcggcatgga aagcagcgtg gcgcttgaca
     3241 ttccaatagc aagaaggtct agcatcgacc cgcctataaa cgtaccgagg atgagctcat
     3301 gcaacctgaa ctgaagccga tcccgaccaa cgaacgcagc ccgggccgcg ccagcgcgcc
     3361 ctttacgtcc gatgtggggg tatcggccag tgcgcagaag gtcctgcgca acacctacct
     3421 gctgc
//
LOCUS       DBNHKE010000110        30303 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_759450, whole genome shotgun sequence.
ACCESSION   DBNHKE010000110 DBNHKE010000000
VERSION     DBNHKE010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 30303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_759450"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..845)
                     /locus_tag="AC16O9_06800"
     CDS             complement(192..845)
                     /locus_tag="AC16O9_06800"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR6516361.1"
                     /translation="MNLEQARHNMVEQQIRPWEVLDPAVLDLLYAVPREDFVPPAYRK
                     LAFADMEIPIGEGEAMMAPKIEARLVQALALRKTDRVLEVGTGSGYLTALLARCAAHV
                     YSLEIRPGLAAFGRDNLARHGAGNVTLEIGDGSRGDPKRAPYDAIVLTGSTPVLPAAL
                     KEQLAPGGRLFAVVGEPPVMVAQLVTCTAPGAYGSAALFDTVLAPLVNAERPPRFTF"
     gene            complement(842..3982)
                     /locus_tag="AC16O9_06805"
     CDS             complement(842..3982)
                     /locus_tag="AC16O9_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6516362.1"
                     /translation="MKFLELLLRNHPLANILFAVVLVMGTLSYLLMPREQDPEINFNW
                     VSIVTTLPGASAEDVEKRVTQPLEDAIKNVADIRFVLSSSRENVSSILVRFNDIAERV
                     FDKRVNDLRREIQNKAQAELPVEVREDPRVLEVTTSNGFPTAQVLVAGIADDETLRRV
                     AREIRNDLERLTGVDQVFALGLHDAELRVEFDPRRLAARGLNAADLADAVSAWFRDTF
                     AGKARAGDDAWLVRVIGQAPEPEYLAGLTLAVPRTGTRIPLEEVARVERARATPRHLA
                     SAHGRPAVLLSVAKKPYTNTLELVELIRRYVADKSAVLGPLGLKLALTDDQTIPTRRA
                     ISVMESNALLGLALVLATCWVFLGTRVSVLVALGIPFSLAGGFAVLHAVGSTLNISVL
                     LGVVIVLGMLVDDAVVVVEAIYYRVQRGAKVLAASLDALRETAAPITTAVLTTIAAFM
                     PLMLLPGIVGKFMFVIPFVVTVTLAISLVEAFWMLPTHLTVMKLDLSRPSRLQRMRHR
                     FTHLLRVRYSRALLVVMRWPKAFAALIVLTFAAALASVAGGLVRMEFFAFDPIRVFYV
                     NVDMPASAPIESTLARVEQVERAVRARLQAGEARALTALAGIKFTDTEPLYGDPYGQL
                     VVSLEPRGVAGREVGEIVQALRKDIESLPGQGKLSFTVLAGGPPVAKPVRVRVRNDDP
                     VALRAAADAVLEIVRAVPGTKDVTDDDVPGRPELRLRLDPDAIRASGLNPGLVARILR
                     LHVDGEIVTEMRDQGEKVQVRVRAAPRSAGDVARLLDDPVSLPAGGSSTTLGSLVRAQ
                     TGIGKGVIKHYNLRRSITIESDLERQRIDTVTANNAIRAGWEKAKGRHPATSLDFSGE
                     LDDIQESLDAMPVLFLLGVGLIYLILAAQFRSYWQPAMILVTVPLAFTGVVFGLLASG
                     NPLSLYTMYGVIALTGIAVNAAIVLIDAANARRRARMSVLHAAVYAARRRVVPIIITS
                     TTTIAGLFSLAVGLGGHSLIWGPMAASIVWGIGFSTVLTLFAMPLLYWGLMPEHRSRR
                     GGATPYNADPSPPTDR"
     gene            complement(3979..4956)
                     /locus_tag="AC16O9_06810"
     CDS             complement(3979..4956)
                     /locus_tag="AC16O9_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6516363.1"
                     /translation="MMLAALLCSAAAAQDPPAATVKPLAEAAIYPERDAAAQVVSLNE
                     SRIAAEIAGRIEAVPVEVGQRIERGALIARIDCRDHDLARARAAAVLTAARSRLALAD
                     QQLARARELQAQGFFAEEALAARITEVEVLRADAAQAEAQLGAAARAVGKCVVRAPFA
                     AIVRERLGRVGELAAPGTPLAALVDLERIEVAALVQTKDTRSLERATEVRFLGPGGAV
                     PLVLERVSPVIDPQTRTAEARLRFAGTAAAPGASGRITWRDPNAHVPPELVVRRAGRL
                     GVFIDDGGTARFHALLEAQEGRPARVDLPPSAAIVVKGQLALQDGQRLR"
     regulatory      5047..5158
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5150..7030
                     /gene="thiC"
                     /locus_tag="AC16O9_06815"
     CDS             5150..7030
                     /gene="thiC"
                     /locus_tag="AC16O9_06815"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR6516364.1"
                     /translation="MNANPKFLAATAHVDAAAVKPLPNSRKVYVEGSRPDIRVPMREI
                     SQADTPSMFGGEKNPPLYVYDCSGPYTDPAATIDIRSGLAPLRQQWIEARGDTQVLPG
                     PSSQYGIERLNDAKLAELRFNLKRAPRRARGGMNVSQMHYAKRGIVTPEMEFIAIREN
                     LERERYIESLKATGKTGEKMLELLMKRHRGESFGASIPERITAEFVRDEVARGRAIIP
                     ANVNHPESEPMIIGRNFLVKVNANIGNSAVSSGIGEEVEKMTWAIRWGADTVMDLSTG
                     KHIHETREWIVRNAPVPIGTVPIYQALEKVDGKAEELTWEIYRDTLIEQAEQGVDYFT
                     IHAGVLLRYVPLTAKRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMRAYDVS
                     FSLGDGLRPGSGYDANDEAQLSELRTLGELTQVAWKHDVQVMIEGPGHVPMQLIKENM
                     DIQLKECHEAPFYTLGPLTTDIAPGYDHITSAIGAAQIGWYGTAMLCYVTPKEHLGLP
                     NKKDVKDGIMAYKIAAHAADLAKGHPGAQVRDNAVSKARFEFRWDDQFNLGLDPDTAK
                     DFHDETLPKDSMKVAHFCSMCGPHFCSMKITQDVREYVEKGMQEKSVEFVRKGAEIYQ
                     KT"
     gene            7027..7848
                     /locus_tag="AC16O9_06820"
     CDS             7027..7848
                     /locus_tag="AC16O9_06820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6516365.1"
                     /translation="MSGESLLVPLGKIALAVAIGVPLIVYFAQDSLIFLRQPIAQARR
                     AEIGRNIPQASSVFVTATDGTRVHAWHYRSGPQLVLYFGGNAEEVSWMLEAVRAETAG
                     VSWLLVDYRGYGQSEGAPSEKALVADALALYDHAAKLPGVDPERIYAFGRSLGSGPAV
                     ALAAHRPLAGIVLVTPFDSLAAVAKRYYWYLPVDWMLKHRFDSITLAPRLQTPLLCLI
                     APRDEVIPAEHGERLYAAWGGPKRRILLEEASHNTTDAHPQFWPPIREMLARRAH"
     gene            7854..8648
                     /locus_tag="AC16O9_06825"
     CDS             7854..8648
                     /locus_tag="AC16O9_06825"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6516366.1"
                     /translation="MDSSVVHALILGLVEGLTEFLPISSTGHLILASDLLGFNDERAK
                     VFNVAIQTGAMLAVVWEYRRRFLSVDRQLYRNLIVAFIPAALLGLLFGGYIKQFLFSP
                     VPVALAFIVGGVIILLVDRGTRVARIESTRAMTWKDALKVGVAQCFALIPGTSRSGAT
                     IIGGMLFGLSRPAATEFSFFLAVPTLVAAGAYDLWRHQGLFAAEHLHMLAVGTAMSFV
                     SALIVIRWLVRYVATHDFLPFAWYRIAFGVIVLVTAYSGIVQWSAP"
     gene            complement(8645..9091)
                     /locus_tag="AC16O9_06830"
     CDS             complement(8645..9091)
                     /locus_tag="AC16O9_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6516367.1"
                     /translation="MTDIDEIRRRAAERGRQLGLAYAGALTPAEAFALMRSGAKLVDV
                     RTKPELQYVGYVPGSVLVEWQTYPGNALNPEFLGQLSGTATPADTLLFLCRSGVRSHY
                     AAAAAAQAGYADSYNVLEGFEGDKDPEQHRNTVGGWRKAGLPWVQG"
     gene            9324..10739
                     /gene="glnA"
                     /locus_tag="AC16O9_06835"
                     /pseudo
     CDS             9324..10739
                     /gene="glnA"
                     /locus_tag="AC16O9_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749065.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
     gene            10815..11321
                     /locus_tag="AC16O9_06840"
     CDS             10815..11321
                     /locus_tag="AC16O9_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6516368.1"
                     /translation="MRRFLLSLGVALSVAPWPALAQSSEEITIWSCRDKDGRTHVTNL
                     REDTSGKDCRIVQQTRVQVVPAPKPAAGNRPAAKAAPGSFPKEDSQARASARERQREI
                     LEQELQREETQLADARKELEEQESVRYGDERNYARVLERLQKYKEAVELHQKNVESLK
                     RELANLYR"
     gene            11339..12388
                     /gene="glnL"
                     /locus_tag="AC16O9_06845"
     CDS             11339..12388
                     /gene="glnL"
                     /locus_tag="AC16O9_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR6516369.1"
                     /translation="MAATRFSGLEYLSTAVMALDERATVRYANPAAENLLGVAAKSLL
                     GQAFLPLFENDPALEKALGEALVTHWDYSAQNVSYPRPGRDPLPLACVLTRIDAPGMP
                     LLAELRPIEQQLRQAREERLIGEQQASRELIRNLAHEIKNPLGGLRGSAQLLERELDR
                     PELLEYTQVIIKEADRLQTLMDRLLTPQRQPRIAPLAIHEVLERVRSLVLAEFSSGIA
                     IECDYDPSLPDLEGDKELLIQAVLNVVRNAAQAIAGGGRPGAITLRTRALRQVTLLRQ
                     RHRLALELQVIDDGPGVPEDIRDRIFNPLVSGRDGGSGLGLSLAQTFVHYHRGVIECD
                     SRPGRTVFRIVLPLA"
     gene            12385..13788
                     /gene="ntrC"
                     /locus_tag="AC16O9_06850"
     CDS             12385..13788
                     /gene="ntrC"
                     /locus_tag="AC16O9_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR6516370.1"
                     /translation="MKPVWIVDDDRSIRWVFEKALGREGIAFSSFASAQEALDALNDG
                     APQVLVSDIRMPGPSGLELLQTVKQQHPAVPVIVMTAYSDLESAVAAFQGGAFEYLPK
                     PFDVDQAVELIRRALDESQRDGASAEPMADAPEILGQAPAMQEVFRAIGRLAQSSATV
                     LITGESGSGKELVARALHRHSARATRPFVAINTAAMPKDLLESELFGHERGAFTGAQA
                     QRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSENSFYRVGGNQPIKANVRVIAAT
                     HQDLEQRVREGAFREDLYHRLNVIRVRLPSLRERREDIALLMRHFLARSAQQLGVEAK
                     RLSESAAEHLQRLDFPGNVRQLENLCHWLTVMAPGQVVEFGDLPAELRDPAVSATAAD
                     WVAALEQEAERRLARGETGILEALTRQFERSLIQRALARTAGRRIEAASLLGMGRNTI
                     TRKIQELGIENGADEEK"
     gene            complement(13892..14962)
                     /locus_tag="AC16O9_06855"
     CDS             complement(13892..14962)
                     /locus_tag="AC16O9_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516371.1"
                     /translation="MPSPYKILIFGASYGSLLATKLCLAGHTAHLVCLPAEADLINQE
                     GARVRMPVKGREGLVEVDSRKLTGKLSAGGPAGINPSEFDLVALAMQEPQYRSSGVRE
                     LLEAVAKAKVPCMSIMNMPPLPYLARVPGVDAAACRGCYTDAAVWDSFDPAYVTLCSP
                     DPQASRPPDEKVNVLQVRLPTNFKSARFPSEAHTAILRRLEADIEAARFDTGAGKIEL
                     PVKLKVHDSVFVPLAKWAMLMAGNYRCVTKQGIRSIKEAVHTDIEASRAVYNWVVELC
                     TSLGADAKDLVPFEKYAAAANGLTGPSSAARALYGGAPNIERVDRLVQAIAKQKGMRS
                     AVLDEVVALVDAQLEANRKAKK"
     gene            15073..16668
                     /gene="aceB"
                     /locus_tag="AC16O9_06860"
     CDS             15073..16668
                     /gene="aceB"
                     /locus_tag="AC16O9_06860"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR6516372.1"
                     /translation="MSSPLPKGVAVRAAVTPAFAEILTSEALAFVAALHREFEPRRQQ
                     LLAARAARQKEFDAGKLPDFLPETRKTRESDWKVAPQPKDMLDRRVEITGPTDRKMVI
                     NALNCGASTFMADFEDANCPTWHNMIDGQINLRDAVRRTISFQQGDKSYALNDQTAVL
                     IPRPRGWHLNEKHVTVDGEQVAGGIFDFALYFFHNANELLRRGSGPYFYLPKLESHLE
                     ARLWNDIFKAAQAALGVPQGSIKATVLIETILAAFEMDEILYELKEHSAGLNIGRWDY
                     IFSCIKKFRSNDKFCLADRSQVTMTAPFLRAYALLLVKTCHRRGAPAMGGMAAQIPIK
                     NDPAANEAALEKVRQDKLREVTDGCDGTWVAHPGLVPVAKEVFDRHMPKPNQYDKQRP
                     DVNVTAKELLDFRPEAPITEAGLRNNISVGIQYLGSWLGGNGCVPVFNLMEDAATAEI
                     SRSQIWQWIRSDKGKLEDGRRVTKSLFRHLLSEELPKVRAYVGEEAWEAGNYSESAAL
                     FEKITSDDNYVEFLTLPAYERIN"
     gene            complement(16680..17627)
                     /locus_tag="AC16O9_06865"
     CDS             complement(16680..17627)
                     /locus_tag="AC16O9_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6516373.1"
                     /translation="MRLAYATSGNGPPIVKTGNWFTHLEFEWQSPVWRHVLEGLSERR
                     TLVRYDMRGTGLSDRDPATVSLESYVTDLATVIEAAGHARFPLLGISQGGAASIAYAV
                     RHPERVSHLILFGAYARGTSRRSGAGQGPEAVETYRRLIRDGWGSDDPAFRQVFSAQF
                     MPDGTPEQLRWFSELERASSSAEFAEQSFLVNSSTDITDLLPQVRVPTLILHCRGDRR
                     VPFDQGRELAAAIPDAKLVTLESNNHLFLAHEPAADMFFRELAQFLGDQPPTAWKRSL
                     QSARLQAAAQKMEASPWYKLAGAIAVVATLVSFVVWLVA"
     gene            complement(17722..18093)
                     /locus_tag="AC16O9_06870"
     CDS             complement(17722..18093)
                     /locus_tag="AC16O9_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516374.1"
                     /translation="MDPAGAIGKLGFRRWYERQLYVCHGWLVASLLGAVALFALLEDL
                     SLLELGFKAALVLVAACVSGLFAWYALWRYLGMLVQAQRLSERSTCTRCGTYGRYRLV
                     RATVSSMTVSCRKCENEWTIA"
     gene            complement(18117..18887)
                     /gene="xth"
                     /locus_tag="AC16O9_06875"
     CDS             complement(18117..18887)
                     /gene="xth"
                     /locus_tag="AC16O9_06875"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6516375.1"
                     /translation="MKLATWNVNSVKVRLPQLLEWLATARPDVVCLQELKAEEAKFPR
                     AELEAAGYACAVNGQKTYNGVALLARSPLAGIAPDIPGFADEQRRVIAATVQEVRVVC
                     VYCPNGQAVGSEKYDYKLRWFAALRDYLAAEFARHPRLAVAGDFNVAPEDRDVHDPKA
                     WEGQVHVSEPERAAWRGLLALGLKDSFRLFEQPEKIYTWWDYRMLGFRRNAGLRIDHI
                     LLSPALAERCTAASVDQAPRKLERPSDHAPALAELRTA"
     gene            complement(18907..19479)
                     /locus_tag="AC16O9_06880"
     CDS             complement(18907..19479)
                     /locus_tag="AC16O9_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6516376.1"
                     /translation="MRSVIFAVLLGAACAASAQLYRWTDEKGRVHVTDTPPPPTATNV
                     RKRAADTAPAAQSGADQPYAVQLAAKSYPVTLFTAPDCAPCGAARSLLNQRGVPFREV
                     SVVGEQEQAQLRQAVGSLSVPSLVVGTGTQKGFEQGAYHSMLDLAGYPRTGILPPRNQ
                     AAPKPVPPKPAAASDAPPAEEAPAGPYTPR"
     gene            complement(19512..21539)
                     /locus_tag="AC16O9_06885"
     CDS             complement(19512..21539)
                     /locus_tag="AC16O9_06885"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6516377.1"
                     /translation="MDRLGNNPLLEFSGLPRYADIRPGHVAPAIDLLLAEARAAVARA
                     EQAPPHWDEFVAPLEDATERLGRAWGQVSHLHAVLDSPELRAAYNENLPKLTQFWTEL
                     GHNEALFAKYGALRDSPAFAALTPARRRIVDNALRDFRLGGAELAADQKPRYAQIQDE
                     IARLGAKFSENVLDATNAFSLVIEDAARLGGIPRDVLQSAKQEDGRGWKFTLHMPSYL
                     PVMQYAEDRALREALYRAYATRASELGKPELDNTTLIARLLPLRREAAQMLGYPSYAE
                     LSLVPKMAESPAQVLEFLEDLARRARPHAERDLAELEAFARTELGLERLEMWDIPFAS
                     ERLRERRYAFSDQEVKQYFPEDAVLAGLFRLVETLYGVRIASASAPLWHSEARFFDVR
                     DARGELVGQFYMDLYARETKRGGAWMDDARSRRRLDERLQTPVAHVVCNFSRPVGARP
                     ALFTHDEVLTLFHEFGHGLHHLLTRVDDLGVSGINGVEWDAVELPSQFMENFCWEWDV
                     LQHMTRHVDTGAPLPRSLYDKMLAAKNFQAGLAMLRQIEFAVFDMRLHWDFDPADRGV
                     LDLLRDVRAKIAVIIPPAWNRFANSFSHIFAGGYAAGYYSYKWAEVLSADAYAAFEEA
                     PQILDAATGARFRDQVLAVGGSRPAAESFHAFRGREPRVEALLRHNGMIPA"
     gene            complement(21520..22383)
                     /gene="folD"
                     /locus_tag="AC16O9_06890"
     CDS             complement(21520..22383)
                     /gene="folD"
                     /locus_tag="AC16O9_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR6516378.1"
                     /translation="MAAKIIDGKALSAELRTALKPAVQQLAARGMRPGLAAIVVGDDA
                     ASRVYVRNKVRACEETGVRSESHELPARATEREILERVAALNADPRVHGILVQLPLPR
                     GVDAERVLAAVSPTKDVDGFHADNLGLLLRGTPRFLPCTPAGILRLLEHAGVALAGRR
                     AVVIGRSSIVGKPLALLLLQKDATVTICHSKTEHLAALAREADVLVAAAGVPRLVGAE
                     MVKPGACVVDVGIHRGADGKLVGDVDAAAVLPIAGWLTPVPGGVGPMTVAMLVANTIR
                     ATELALGQTRQ"
     gene            22454..25144
                     /gene="aceE"
                     /locus_tag="AC16O9_06895"
     CDS             22454..25144
                     /gene="aceE"
                     /locus_tag="AC16O9_06895"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6516379.1"
                     /translation="MSAVPLHPAANDSDTLETQEWLDALEAVLEREGPERAHFLLERL
                     VEKARTSGAYIPFSANTAYLNTIPPHLEERSPGDVTLEERIRSICRWNAMVMVVRANK
                     SDDELGGHIASFASVGTLFGTGQQHFWHAPHEGHGGDLVYFQGHSSTGVYARGLLEGR
                     LTEEQLNNFRREVDGKGVSSYPHPWLMPDYWQFPTVSMGLGPIQAIYMARYLKYLHAR
                     GFADTANRKVWVFCGDGEMDEPESLGAIGMAAREKLDNLVFIVNCNLQRLDGPVRGNG
                     KIIQELEGDFRGAGWNVIKVIWGSYWDPLFARDTEGRLLRVMEETVDGEYQNYKANDG
                     AFVRKHFFGKDPKVLELVSRMTDEDIWRLNRGGHDPHKIYAAYAAAVKHRGQPTVILA
                     KTIKGYGLGKQGQAKNPTHQLKKVDTATIKEMRDSFNIPIPDDRLDELPFYVPPPDSP
                     EMQYLHERRKKLGGFLPQRRRKADQSPAVPPLSAFDAVLKGSGEGREISTTMAFVRLL
                     TALVRDKELGKRIVPIVPDEARTFGMEGMFRQLGIFAPEGQKYEPVDKDQVMYYREDK
                     AGQILEEGINEAGAFSSWIAAATSYSHSNVVTVPFYIFYSMFGMQRIGDLAWAAADQR
                     ARGFLLGATAGRTTLNGEGLQHEDGHSHVLSSVIPNCVSYDPTYGYELAVIVHDGLRR
                     MVTEQEDVFYYLTVMNENYEHPPIPKGAEDGILKGMYLLRESKSKAKTRVQLMGSGTI
                     LREVEAAAGLLEKDWGVAADVWSATSFTELRRDGLAAERWNLLHPGDKPRLAYVTQML
                     EKRPAGPVVAASDYIKTFADQIRPFVPRERVYRVLGTDGFGRSDSRAKLRHFFEVDRH
                     FVAVAALQALAEQGDGKAKAVADALKKYRIDPDKPDPTTV"
     gene            25141..26757
                     /gene="aceF"
                     /locus_tag="AC16O9_06900"
     CDS             25141..26757
                     /gene="aceF"
                     /locus_tag="AC16O9_06900"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIR6516380.1"
                     /translation="MREISVPDIGDFKDVEVIEVLVKAGDTIAAEQSLITVESDKATM
                     EIPAPGAGTVKEMRVKVGDKVSQGTPILVLDEAKGDAEPKAAPAPAPQKAQEAPAPAP
                     EKAAVVPAAKSAGPMTVKVPDIGDFKDVAVIEVLVKPGDTVAHEQSLITVESDKATME
                     IPSPAAGKITALKLKVGDKVSEGSTILELEAEGAAPAAPTAKPTKAHPTPAAVPEAAA
                     PHRPPVPKEPQEATHAKPHASPSVRKFARELGVDLTRVKGSGPKGRIVHGDVQAFVKG
                     ALAAVPAAPAAGGGAQPFNLPAWPDVDFAKFGPVEAKPLTRLQKLSGPNLHRNWVSIP
                     HVTQFDEADITELEAFRKSNTAETEKQGFKLTMLAFLVKACVTALRQHPNLNASLDRG
                     GENLILKKYYNIGVAVDTPDGLVVPVVRDADRKGVFDVARELGEISKLARDKKLKPGD
                     MQGGTFSISSLGGIGGTAFTPIINAPEVAILGVSRSVLRPVWSGKEFAPRLMLPLSLS
                     YDHRVIDGAAAARFTSYLASVLVDIRRSLL"
     gene            26758..28158
                     /gene="lpdA"
                     /locus_tag="AC16O9_06905"
     CDS             26758..28158
                     /gene="lpdA"
                     /locus_tag="AC16O9_06905"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6516381.1"
                     /translation="MAELDCDVVVLGAGPGGYSAAFRAADLGLKTVLVERYPTLGGVC
                     LNVGCIPSKALLHTAAVMDAAQALASHGISFGAPQVDLDKLRAFKEKVVGKLTGGLAA
                     MAKMRKVTVVQGVGTFSGANQLAVEGTDRTTVRFKSAIIAAGSQSAKLPFLPDDPRIV
                     DSTGALELKSAPKKLLIIGGGIIGLEMGTVYSTLGARLDVVEMMDGLMLGADRDLVAV
                     WQKFNAKRFDRVMLKSKTTKAEATKAGIRVSFEGQEAQTYDLVLVSVGRVPNGNKIGA
                     DKAGVRVDERGFIAVDSQMRTNVPHIYAIGDIAGNPMLAHKAVHEAHVAAEAAAGQKS
                     HFDARVVPSVAYTDPEIAWVGLTEDEAKKAGTKIGVAKFPWSASGRAIANARDEGFTK
                     LIFDAESHRILGGGIVGTGAGDLISELALAVEMGADATDIGKTIHPHPTVSESIGMAA
                     EAFEGVCTDLPPQRKK"
     gene            28155..28823
                     /gene="rpiA"
                     /locus_tag="AC16O9_06910"
     CDS             28155..28823
                     /gene="rpiA"
                     /locus_tag="AC16O9_06910"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR6516382.1"
                     /translation="MTQDELKQLVAREALKHVVEDAVVGVGSGSTVNFFIDALAGIKG
                     RIEGAVAASDASAERLKKHGIRVFDLNAVDELPVYVDGADEVNEHLYMIKGGGGALTR
                     EKIIAAVARKFVCICDASKLVGVLGKFPLPVEVIPMARGHVGRELRRLGGHPELREGF
                     KTDNGNLVIDCRGLTILDPPRLEAELNNIAGVVTNGLFARRPADLLLLAEAGAVRAVV
                     APKA"
     gene            28860..29558
                     /gene="phoU"
                     /locus_tag="AC16O9_06915"
     CDS             28860..29558
                     /gene="phoU"
                     /locus_tag="AC16O9_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR6516383.1"
                     /translation="MPEHTSKQFDAELESVRSRVLQMGGLVEEQIIRAMDVLAAGDMQ
                     QIEKVIADDHRVNAMEVELDELCSHIIARRQPAAVDLRLLITVIKTITDLERIGDEAE
                     KIGRMAKLIHAAERRHMPRLDLSPVAERALAMLRQSLDAFARLDVSEAMQVVKQDHEV
                     DDAFRAIMRQLITFMMEDPRTITRCLEVLFVAKAIERIGDHAKNMAEYVIYMVKGRDV
                     RHTSIEEVEKEILR"
     gene            complement(29627..29899)
                     /locus_tag="AC16O9_06920"
     CDS             complement(29627..29899)
                     /locus_tag="AC16O9_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR6516384.1"
                     /translation="MARTVKCIKLGREAEGLDFPPYPGELGKRIFENVSKEAWAQWLR
                     HQTMLVNENRLNLADIRARKYLAEQMEKHFFGEGADVASGYVPPSN"
     gene            complement(29917..>30303)
                     /locus_tag="AC16O9_06925"
     CDS             complement(29917..>30303)
                     /locus_tag="AC16O9_06925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6516385.1"
                     /translation="EPLEADGTLVKRGRERLEREIGNFIVLEHDGALLGCAALYPLPA
                     KSGELACLAVAPGARDAGHGELLLNACETRARQQKIRRLYALTTRAEHWFLEQGFKPA
                     SPAALPEERQSLYNWRRSSKVFLKRI"
BASE COUNT         5096 a        10189 c        10451 g         4567 t
ORIGIN      
        1 gtactgcgcg tcgtagccgc gcgcgtcctg gtagacctgg gtgaggttct ccgcccccgc
       61 ggcgaggggc gcgcaggcaa gcagggcgag ggcgaggcgt cggatcatgg ggtgtcggct
      121 ctcagtagac gggcatgcgg ggtcgatcgc gcgcgcccag gcatcccgcg gcgtcaggtt
      181 gcgcatcggc gctagaaggt gaaacgcggc gggcgctccg cgttgaccaa gggggcgagc
      241 accgtgtcga acagcgccgc gctgccgtag gcgccgggtg ccgtgcaggt gacgagctgc
      301 gccaccatta ccggcggctc tccgaccacc gcgaacagtc gcccgcccgg tgcgagctgc
      361 tccttgagcg cggcgggcag caccggcgtc gagccggtga gcacgatcgc gtcgtagggc
      421 gcgcgcttcg ggtcgccgcg cgatccgtcg ccgatctcca gcgtcacgtt gccggcgccg
      481 tggcgcgcga ggttgtcgcg gccgaaggcg gcgagccccg gccggatctc gagcgagtag
      541 acatgcgccg cgcagcgcgc gagaagcgct gtgaggtagc cgctgccggt gcccacctcg
      601 agcacgcggt cggtcttgcg aagcgccagc gcctggacga ggcgcgcctc gatcttcggc
      661 gccatcatcg cttcgccttc gccgatcggg atctccatgt cggcgaacgc gagcttgcga
      721 taggccggcg gcacgaaatc ctcgcgcggc accgcgtaga gcaggtcgag caccgccgga
      781 tccagcacct cccaggggcg gatctgctgc tcgaccatgt tgtggcgggc ctgttcgagg
      841 ttcatcggtc ggtgggcggg gagggatcgg cattataagg ggtggccccg ccgcgccgcg
      901 agcggtgctc gggcatcagc ccccagtaga gcagcggcat ggcgaacagc gtcagcaccg
      961 tcgaaaaccc gataccccag acgatggagg ccgccatcgg cccccagatg agcgagtgcc
     1021 cgcccaatcc gacggcgagc gagaacagcc cggcgatggt cgtggtgctg gtgatgatga
     1081 tcggcaccac gcgccggcgg gcggcgtaca cggcggcgtg cagcacgctc atgcgcgcgc
     1141 ggcggcgcgc attggccgcg tcgatcagca cgatggcggc gttcaccgcg atgccggtca
     1201 gcgcaatgac gccatacatc gtgtacagcg acaggggatt gccggaggcc agcagcccga
     1261 acacgacgcc ggtgaaggca agcggcacgg tgaccaggat catcgccggc tgccagtagc
     1321 tgcggaactg cgctgcgagg atcaggtaga tcaggcccac tccgagcaaa aacagcaccg
     1381 gcatcgcgtc caggctctcc tggatgtcgt cgagctcgcc ggagaaatcc agcgaagtcg
     1441 cgggatggcg gcccttcgcc ttttcccagc cagcgcggat cgcattgttg gcggtcacgg
     1501 tatcgatgcg ctggcgctcg aggtccgact cgatggtgat cgagcggcgc aggttgtagt
     1561 gcttgatcac gcccttgccg atgccggtct gcgcgcgcac cagcgagccg agcgtcgtgc
     1621 tcgagccacc ggcgggcagc gataccggat cgtcgagcag ccgcgccacg tcgcccgcgc
     1681 tgcgcggcgc cgcgcgcacg cgcacctgca ccttctcgcc ctggtcgcgc atctcggtga
     1741 cgatctcgcc gtccacgtgc aggcgcagga tgcgcgcgac gagccccggg ttgaggccgc
     1801 tcgcgcggat cgcatcggga tcgaggcgca ggcgcagctc cgggcgcccg ggcacgtcgt
     1861 cgtcggtcac gtccttggtg cccggaaccg cgcgcacgat ctcgagcacc gcgtccgccg
     1921 cggcgcgcag tgccacgggg tcgtcgttgc gcacgcgcac gcggaccggc ttggccaccg
     1981 gcggcccgcc cgcgagcacc gtgaagctca gcttgccctg cccgggcagg gactcgatat
     2041 ccttgcgcag cgcctgcacg atctccccga cctcgcggcc cgcgacgccg cggggctcga
     2101 gcgacaccac cagctgcccg tagggatcgc cgtagagcgg ctcggtgtcg gtgaacttga
     2161 ttcccgcgag cgccgtgagc gcgcgcgctt cgccggcttg caggcgcgcg cgcaccgcgc
     2221 gctccacctg ctcgacgcgg gcgagcgtcg actcgatcgg cgcgctcgcc ggcatgtcca
     2281 cgttgacgta gaacacccgg atcggatcga aggcgaagaa ctccatgcgc accaggccac
     2341 ccgcgaccga agcgagggcg gcggcaaaag tcagcacgat gagtgccgca aaggccttgg
     2401 gccagcgcat cacgacgagc agcgcgcgcg agtagcgcac gcgcagcagg tgcgtgaagc
     2461 ggtggcgcat gcgctgcagc cgcgagggcc gggacaggtc gagcttcatc acggtgaggt
     2521 gcgtcggcag catccagaag gcctcgacca ggctgatggc cagcgtcacc gtgaccacga
     2581 acggaatgac gaacatgaac ttgccgacga tgcccggcaa gagcatgagc ggcatgaagg
     2641 cggcgatcgt cgtcagcacc gcggtagtga tcggagcggc ggtctcgcgc agcgcatcga
     2701 gcgaggcggc gagcaccttg gcgccccgct gcacgcggta gtagatcgcc tccaccacca
     2761 ccacggcatc gtccaccagc atgccgagca cgatcaccac cccgagcagc acggagatgt
     2821 tgagcgtgga gcccacggcg tgcagcaccg cgaagccgcc ggcaagcgag aacggaatgc
     2881 cgagcgccac cagcaccgac acgcgcgtgc cgaggaacac ccagcaggtg gcgagcacca
     2941 gcgcgagacc cagcagggcg tttgactcca tgaccgagat cgcgcgccgc gtcggaatgg
     3001 tctgatcgtc ggtgagcgcg agcttcaggc cgagcgggcc gagcacggcg ctcttgtcgg
     3061 cgacgtaccg gcggatcagc tcgaccagct cgagcgtgtt ggtatagggt ttcttggcaa
     3121 cggagagcag caccgccggc cggccatgcg ccgaggcgag atggcgcggg gtggcgcgcg
     3181 cacgctcgac gcgcgccact tcttccagag ggatgcgcgt gcccgtgcgc ggcacggcga
     3241 gcgtcagccc ggccagatac tcgggctcgg gcgcctggcc gatgacgcgc accagccagg
     3301 cgtcgtcgcc ggcgcgcgcc ttgccggcga acgtgtcgcg aaaccaagca ctcacggcat
     3361 cggcgaggtc ggccgcgttg aggccgcgcg ccgcaaggcg acgcggatca aactcgacgc
     3421 gcagctcggc gtcgtgcagg ccgagggcga agacctgatc cacgccggtg agccgttcga
     3481 ggtcgttgcg gatctcgcgc gccacgcgcc gcagcgtttc gtcgtcggcg attccggcca
     3541 ccagcacctg cgccgtggga aagccgttcg aggtggtgac ctcgagcacg cgcgggtcct
     3601 cgcgcacctc gacgggcagc tcggcctgtg ccttgttctg gatttcgcgc cgcaggtcgt
     3661 tcacgcgctt gtcgaacacg cgctccgcga tgtcgttgaa gcgcaccagg atcgaggaca
     3721 cgttctcgcg gctggaagac agcacgaagc ggatgtcggc gacgttcttg atcgcgtcct
     3781 cgagcggttg cgtgacgcgc ttctccacgt cctcggccga ggcgccgggc agcgtggtga
     3841 cgatgctcac ccagttgaag ttgatctccg ggtcctgctc gcgcggcatg agcaggtagg
     3901 agagcgtgcc catcaccagc accaccgcga acaggatgtt cgcgagcgga tggttcctga
     3961 gcagcagctc taggaacttc atctgaggcg ctggccgtcc tgcagcgcga gctgcccctt
     4021 gactacgatg gccgcgctcg gcggcaggtc gacgcgcgcc ggccggcctt cctgcgcttc
     4081 gagcagggcg tggaagcgcg ccgtaccgcc gtcatcgatg aacacgccga gacgcccggc
     4141 gcggcgcacc accagctcgg gcggcacgtg cgcattggga tcgcgccagg tgatgcgccc
     4201 ggaggcgccc ggcgccgccg cggtcccggc gaagcgcaag cgcgcctcgg cggtgcgcgt
     4261 ctgcgggtcg atgaccggcg agacgcgctc gaggacgagc ggcacggcgc cgccgggccc
     4321 gagaaagcgc acttcggttg cgcgctcgag ggagcgcgtg tccttggtct gcaccagcgc
     4381 cgcgacctcg atgcgctcca ggtcgacgag cgccgcgagc ggcgtgccgg gcgccgcaag
     4441 ctcgcccacg cgcccgagcc gctcgcgcac gatggccgcg aacggtgcgc gcaccacgca
     4501 cttgcccacg gcgcgcgccg ccgcgccaag ctgcgcctcg gcctgcgcgg cgtcggcgcg
     4561 cagcacctcg acttcggtga tgcgcgcggc gagcgcctct tcggcgaaga atccctgcgc
     4621 ctgcagctcg cgcgcccgcg cgagctgctg gtcggcgagc gccaggcgcg agcgcgcggc
     4681 ggtgagcacc gcggcggcac gtgcgcgcgc gagatcatgg tcgcggcagt cgatgcgcgc
     4741 gatcagcgcc ccgcgctcga tgcgctggcc gacttcgacc ggcacggcct cgatgcgccc
     4801 agcgatctcg gcggcgatgc gcgattcgtt cagcgacacc acctgcgcag ccgcgtcgcg
     4861 ctcgggataa atggccgcct ccgccagggg ctttaccgtc gcggcagggg ggtcctgcgc
     4921 cgctgccgcc gagcaaagca gcgcagcgag catcatcgga agtacacgca tgaggccagt
     4981 gcagtgcaat ggtagcagcg caggcgcgga attcttgttt cttggggtac tttgcagtag
     5041 cgttgtggcc ttacgcgggg gtcctggtcc ggcgcgtttg aaacgcgctc ccgggtgaga
     5101 aagacccttt gaacctgatg cgggtaatcc cgccgaagga agcgaacgaa tgaacgcgaa
     5161 tcccaagttc ctggctgcca ccgcccacgt cgacgcggcg gcggtcaagc ccctccccaa
     5221 ctcgcgcaag gtttacgtcg agggctcgcg gcccgacatc cgcgtgccga tgcgcgagat
     5281 ttcgcaggcc gacacgccga gcatgttcgg cggcgagaag aacccgccgc tctacgtcta
     5341 cgactgctcc ggaccctata ccgatccggc ggcgacgatc gacatccgct cgggtctggc
     5401 gccgctgcgg cagcagtgga tcgaggcgcg cggcgacacc caggtgctgc cgggtccgtc
     5461 gtcgcagtac ggcatcgagc gcctcaacga cgccaagctc gccgagctgc gcttcaatct
     5521 gaagcgcgcg ccgcgccgcg cgcgcggcgg catgaacgtc tcgcagatgc actacgcgaa
     5581 gcgcgggatc gtcacgccgg agatggagtt catcgccatc cgcgagaacc tcgagcgcga
     5641 acgctatatc gagagcctga aggccaccgg caagacgggc gagaagatgc tcgagctgct
     5701 gatgaagcgg catcgcggcg agtcgttcgg cgcgtcgatt cccgagcgca tcaccgccga
     5761 gttcgtgcgc gacgaggtgg cgcgcgggcg cgccatcatt ccggccaatg tcaaccaccc
     5821 ggaaagcgag ccgatgatca tcggccgcaa cttcctggtg aaggtgaacg cgaatatcgg
     5881 caactcggcg gtgtccagcg gcatcggcga ggaagtggag aagatgacct gggcgatccg
     5941 ctggggcgcc gacacggtga tggatctctc caccggcaag cacatccacg agacgcgcga
     6001 gtggatcgtg cgcaacgccc cggtgccgat cggcaccgtg ccgatctacc aggcgctgga
     6061 gaaggtcgac ggcaaggccg aggagctcac ctgggagatc taccgcgaca cgctgatcga
     6121 gcaggccgag cagggcgtgg actacttcac catccacgcc ggcgtgctcc tgcgctacgt
     6181 gccgctcacc gcgaagcgca tgaccggcat cgtctcgcgc ggcggctcga tcatggccaa
     6241 gtggtgcctc gcgcaccaca aggagagctt tctctacacg cacttcgagg acatctgcga
     6301 gatcatgagg gcgtacgacg tgagcttctc gctcggcgat ggcctgcgtc ccggctcggg
     6361 ctacgacgcg aacgatgaag cccagttgtc ggagctgcgc acgctcgggg agctgaccca
     6421 ggtcgcgtgg aagcacgacg tccaggtgat gatcgaaggc ccggggcacg tgccgatgca
     6481 gctgatcaag gagaacatgg acatccagct gaaggagtgc cacgaggcgc ccttctacac
     6541 gctggggccc ctcaccaccg acatcgcgcc gggctatgac cacattacca gcgccatcgg
     6601 cgccgcgcag atcggctggt acggcacggc gatgctgtgc tacgtcacgc ccaaggagca
     6661 cctcgggctg ccgaacaaga aggacgtgaa ggacggcatc atggcctaca agatcgccgc
     6721 gcacgcggcc gacctcgcca agggacatcc gggcgcgcag gtccgcgaca acgcggtctc
     6781 caaggcgcgc ttcgaattcc gctgggacga tcagttcaac ctcggcctcg atccggacac
     6841 cgccaaggac ttccacgacg agacgctgcc caaggactcg atgaaggtgg cgcacttctg
     6901 ctcgatgtgc gggccgcact tctgctcgat gaagatcacg caggacgtgc gcgagtacgt
     6961 cgagaagggc atgcaggaga agtcggtcga gttcgtgcga aagggcgccg agatctacca
     7021 gaagacgtga gcggcgagag cctgcttgtg ccgctgggca agatcgcgct ggcggtggcg
     7081 atcggcgtgc cgctcatcgt gtatttcgcg caggacagcc tgatcttctt gcgccagccg
     7141 attgcgcaag cgcgccgcgc cgagatcggg agaaacattc cccaggccag tagcgtcttc
     7201 gtcaccgcca cggacggcac gcgcgtgcat gcctggcact accggtccgg tccgcagctg
     7261 gtgctctact tcggcggcaa tgcagaagaa gtgtcgtgga tgctggaagc cgtgcgcgcc
     7321 gagaccgcgg gcgtgtcctg gctgctcgtg gactatcgcg gctacggcca gagcgagggc
     7381 gcgccctccg aaaaagccct cgtcgcggac gcgctcgcgc tctatgatca cgcagcgaag
     7441 ctgccgggcg tggatccaga gcgcatctat gctttcgggc gcagccttgg cagcggtccg
     7501 gcggtggcgc tcgccgcgca caggcccttg gcgggaatcg tgcttgtcac cccctttgac
     7561 agccttgcgg ctgtggccaa gcgctattac tggtacctgc ccgtggactg gatgctcaag
     7621 caccgcttcg attcgatcac gctcgcgccg cgcttgcaga cgccgctcct ctgcctcatc
     7681 gctccgcggg acgaggtgat cccggccgag cacggcgagc gcctgtacgc ggcctggggt
     7741 gggccgaaac gaaggatcct cctggaggag gcgagccaca acaccaccga cgcgcacccg
     7801 cagttctggc cgccgatccg cgaaatgctg gcgcggcgcg cgcattgacg tcgatggatt
     7861 caagcgtagt ccacgcgctg atcctcggcc tggtcgaagg gttgaccgag ttcctgccga
     7921 tctcgagcac cggccacctg atcctcgcca gcgacctgct gggattcaac gacgagcgcg
     7981 ccaaggtgtt caacgtcgcc atacagaccg gcgccatgct ggcggtggtg tgggaatacc
     8041 ggcggcgctt tctcagcgtc gaccgtcagc tctatcgcaa cctgatcgtc gccttcatcc
     8101 cggccgcgct gctgggactg ctcttcggcg gctacatcaa gcagttcctg ttctcgccgg
     8161 tgccggtggc gctcgctttc atcgtcggtg gcgtgatcat tctgctggtc gatcggggca
     8221 cgcgcgtagc gcgtatcgag tcgacaaggg cgatgacatg gaaggacgcg ctgaaggtcg
     8281 gcgtcgcgca gtgcttcgcg ctcattcccg ggacttcacg ctcgggggcc accattatcg
     8341 gcggcatgct gttcgggctg tcgcgtcctg cggcgacgga gttctcgttc tttctcgccg
     8401 tgccgacgct ggtcgccgcg ggcgcttacg acctgtggcg gcatcagggc ctgttcgcgg
     8461 ccgagcacct tcatatgctc gccgtcggca cggcgatgtc gtttgtatcc gcgctgatcg
     8521 tgatccgctg gttggtgcgc tacgtcgcga cgcacgattt cctgccgttc gcgtggtacc
     8581 gcatcgcgtt cggggtcatc gtcctcgtga ccgcgtattc gggcatagtg cagtggtcgg
     8641 cgccctagcc ctgcacccag ggcaacccgg ccttgcgcca gccgcccacg gtgttgcgat
     8701 gctgctcggg gtccttgtcg ccttcgaaac cttccagcac gttgtaggag tcggcatagc
     8761 cggcctgcgc cgcggcggcc gccgcgtagt gcgagcgaac gccggaacgg cacagaaaca
     8821 gcaacgtatc ggcgggcgtc gcggttccgg agagctggcc caggaactcc ggattgagcg
     8881 cattgccggg gtaggtctgc cattccacga gtacgctgcc gggcacgtat cccacgtact
     8941 gcagctcggg cttggtgcgc acatcgacca gcttcgcgcc cgaccgcatg agcgcaaatg
     9001 cctccgcggg cgtgagtgcg ccggcatagg ccagcccaag ctggcggccg cgctcggccg
     9061 cccggcgcct gatctcgtcg atgtcggtca ttgctgctct gcattatagg agctcgaggc
     9121 gggtgcatcc ggggcaccag cttggtgccg acgacgagaa aatgcaccag cattgtgcga
     9181 atgcttgatt tagaatttcc gcagcatggc gcggcagggg cggatcgggg gagaacgcct
     9241 cgtggcatgg aaactgctcc atttttcgcg tccggccgcc gcgagcggcc gcatcccatt
     9301 gcacaaactc aggagatctg cagatgtcca tgacggccga tgacgtcttg aaaatggcga
     9361 aggaaaagga ggtcaagttc gtcgacttcc gctttaccga cacgcgcggc aaggagcagc
     9421 acgtcagcgt gccgatccgg cacttcaaca aggaaaagtt cgagaacgga cacgccttcg
     9481 acggctcgtc gatcgccggc tggaagggta tccaggcctc cgacatgctg ctgatgccgg
     9541 acccctcgac cgcacgcatg gatccgttca gcgacgaagc ggtcctgaac atcagttgcg
     9601 acgtctatga gcccaccgac ggcaagccct atgatcgctg cccgcgcggc atcgcgcggc
     9661 gcgcagaggc gttcctcaag tcgagcggcc tgggcgacac ggcctacttc ggccccgagc
     9721 ccgagttctt catcttcgac ggcgtcacct ggcaggtgga catgtcgggc tgcttcgtga
     9781 agatcaagtc ggaggaggcg ccctggtcca cgggcatcga ctatgagtcg ggcaacatgg
     9841 cccaccgcgc gccagtgaaa ggcggctact tcccggtgcc gccggccgat accctcgccg
     9901 acgtccgcaa cgccatgtgc ctcgcgctcg aacagcaggg cgtggaggtc gaggtgcacc
     9961 atcatgaggt ggccgcggcc ggccagtgcg agatcggcac gcgctttgcg ccgctcgtgc
    10021 agcgcgccga ctggatgcag atcctcaagt acacggtgtg gaacgtggcc gcgtcctacg
    10081 gcaagaccgc gaccttcatg cccaagcccg tggtgggcga caacggctcc ggcatgcacg
    10141 tgcaccaatc ggtgtggaag ggcggccaga acctctttgc gggcaacggc tatgcgggcc
    10201 tctcggagtt cgcgctgttc tacatcgggg gcgtcatcaa gcatgccaag gcgctgaacg
    10261 cgatcaccaa cccgggcacc aacagctaga agcgcctggt gccggggttc gaggcgccga
    10321 tcaacctcgc ctactcggcg aagaaccgct cggcctcgtg ccgtatcccg tacgtgtcga
    10381 gcccgaaggc gcggcgcgtg gaagtgcgct ttcccgatcc gaccgccaat gcctacctcg
    10441 ccttcgcggc gttgctgatg gcgggcctgg acggcgtgca gaacaagatc caccccggcg
    10501 atccgatcga caagaacatg tacgacctgc cgccggaaga agccgccaag gtgccgcacg
    10561 tgtgcgcttc gctcgaggaa gcgctgcaga gcctcgagaa ggaccacgcg ttcctcacca
    10621 agggcggcgt gttcagcgag gatttcctcg agtcctacat gacgctgaag gaagaggagc
    10681 tgacgcgctt tcgcatgacc acgcacccgg tcgaattcga tatgtactac tcgtcctgag
    10741 cgcgcgccga gcacaacgct aggatcgggg acgggcttgc ccgtccccgc gctttttctg
    10801 ttatgttatt gatcgtgcgc cgatttttgc tgtcgctggg agttgcgctg agcgttgcgc
    10861 cgtggccggc gctggcgcag tcatcggaag agatcaccat ctggagctgt cgcgacaagg
    10921 acggccgcac gcacgtcacc aacctgcgcg aagacacgag cggcaaggat tgccgcatcg
    10981 tgcagcagac gcgggtgcag gtggtgccgg cgcccaagcc ggccgccggc aaccggcccg
    11041 cggccaaggc ggcgccgggc agctttccga aggaagactc gcaggcgcgc gcctccgcgc
    11101 gcgagcgcca gcgcgagatc ctggagcagg agctgcagcg cgaggagacg cagcttgccg
    11161 acgcgcgcaa ggaactcgag gagcaggagt cggtgcgcta cggcgacgag cgcaactatg
    11221 cgcgggtgct cgagcggctg cagaagtaca aggaagcggt ggaactgcac cagaagaacg
    11281 tcgaatcgct caaacgcgag ctcgccaacc tgtaccggtg agcgcggcgg cctgacccgt
    11341 ggccgcgacc cgcttctccg gcctggagta cctttcgacc gccgtcatgg cgctcgatga
    11401 gcgcgcaacg gtgcgctatg ccaacccggc ggcggagaac ctcctcggcg tcgcggcgaa
    11461 gagcctcctc gggcaggcct tcctgccgct attcgaaaac gatcccgcgc tggagaaggc
    11521 gctcggcgag gcgctggtca cgcactggga ctattcggcg cagaacgtga gctacccgcg
    11581 gcctgggcgc gatccgctgc cgctcgcctg cgtgctgacg cgcatcgacg cccccggcat
    11641 gccgctgctc gccgagctgc gcccgatcga gcagcagctg cgccaggcgc gcgaagagcg
    11701 tctgatcggc gagcagcagg cgagccgcga gctgatccgc aacctcgccc atgagatcaa
    11761 gaaccccttg ggggggctgc gtggctcggc gcaactgctc gagcgggagc tcgaccgacc
    11821 cgagctgctc gagtacacac aggtgatcat caaggaggcc gaccgcctgc agacgctgat
    11881 ggaccggttg ctgacgccgc agcgccagcc gcgcatcgcg ccgctcgcca tccacgaggt
    11941 cctggagcgc gtgcgcagcc tcgtgctggc cgaattctcg agcggcatcg ccatcgagtg
    12001 cgactacgac ccgagcctgc ccgacctcga gggcgacaag gaactgctga tccaggcggt
    12061 gctgaacgtg gtgcgcaacg ctgcccaggc tatcgccggc ggcggccgac ccggcgccat
    12121 cacgctgcgc acccgggcgt tgcgccaggt cacgctgctc aggcagcgcc acaggctggc
    12181 actagaattg caagtgatcg atgacgggcc cggcgtccct gaggacatcc gggaccggat
    12241 attcaacccc ctggtctcgg ggcgggacgg cggcagcggg ctgggcttat cgctcgcaca
    12301 gaccttcgtt cactaccacc ggggagtcat cgagtgcgac agccgccccg gccgcaccgt
    12361 tttcaggatc gtgctgccgc tggcatgaaa ccggtctgga tcgtcgacga cgaccgctcc
    12421 atccgctggg tcttcgagaa ggccctcggg cgcgaaggca tcgccttcag ttccttcgca
    12481 tcggcgcagg aagcactcga cgcgctgaac gacggcgcgc ctcaggtgct ggtgtccgac
    12541 attcgcatgc cggggccctc ggggctcgaa ctcctgcaaa cggtcaagca gcagcatccg
    12601 gcggtgcccg tgatcgtgat gaccgcgtac tccgacctcg agtccgcggt ggcggcgttt
    12661 cagggcggcg ccttcgagta cctgcctaag ccgttcgatg tcgatcaagc ggtggaattg
    12721 atccgccgcg cgctggacga aagccagcgc gacggggcga gcgcggaacc gatggccgac
    12781 gcgccggaga tcctcggcca ggcgccggcg atgcaagagg tgttccgcgc catcggccgg
    12841 ctggcgcagt cgagcgcgac cgtgctgatt accggcgagt ccggctcggg caaggagctc
    12901 gtcgcgcgcg ccctgcaccg tcatagtgcg cgcgcgacgc gcccgttcgt cgccatcaac
    12961 acggcggcca tgcccaagga cctgctcgaa tcggagctct tcggccacga gcgcggcgcg
    13021 ttcacgggcg cccaggcgca gcgccgcggc cgcttcgagc aggccgaggg cgggacgctg
    13081 tttctcgacg agatcggcga catgccggcg gagttgcaga cgcggttgct gcgcgtgctt
    13141 tcggagaaca gcttctaccg cgtcggcggg aaccagccga tcaaggccaa cgtgcgcgtc
    13201 atcgccgcca cgcaccaaga cctcgagcag cgggtgcgcg agggcgcctt ccgcgaggac
    13261 ttgtaccacc ggctgaacgt gatccgcgtg cgcctgcctt cgctcaggga gcgccgcgag
    13321 gacatcgcgc tgctcatgcg ccactttctc gcgcgcagcg cgcagcagct cggcgtggag
    13381 gcgaagcgcc tgtcggagag cgcagccgag cacctgcagc ggctggattt tcccggcaac
    13441 gtgcgtcagc tcgagaacct gtgccactgg cttacggtga tggcgcccgg gcaggtggtg
    13501 gagttcggcg acctgcccgc ggagctccgc gatccggccg tgtccgcgac ggcggccgac
    13561 tgggtggccg cgctcgagca ggaagcggag cggcgcctgg cgcgcggcga aaccgggatc
    13621 ctcgaggcgc tcacccggca gttcgagcgc agcctgattc agcgcgcgct tgcgcgcacg
    13681 gccgggcgcc gcatcgaagc ggccagcctc ctcggcatgg gccgcaacac gatcacgcga
    13741 aagatccagg agctgggcat cgagaacggc gccgacgagg agaagtaggc gccttcgttt
    13801 ggtcttctat ggtctcggct tttctggcgg ggtgaatctt cccgccagaa aagccgagat
    13861 gtattgaaag gcgaaaaccc cgccgaagcg cctacttctt cgccttgcgg ttcgcctcca
    13921 gctgcgcgtc caccagcgcc acgacctcgt cgagcaccgc cgagcgcatg cccttttgct
    13981 tggcgatcgc ctgcaccagg cgatccacgc gctcgatgtt gggcgctccg ccgtagagcg
    14041 cgcgcgcggc ggacgagggg ccggtgaggc cgttggcggc ggccgcgtac ttttcgaacg
    14101 gcaccaggtc cttcgcgtcc gcgccgagcg aggtgcacag ctcgaccacc cagttgtaga
    14161 ccgcgcgcga ggcctcgatg tccgtgtgca cggcttcctt gatggagcgg atgccctgct
    14221 tggtgacgca gcggtagttg ccggccatca gcatggccca cttagcgagc ggcacgaaca
    14281 ccgagtcgtg caccttgagc ttcaccggca gctcgatctt gccggcgccg gtgtcgaagc
    14341 gcgcggcctc gatgtcggct tcgagccggc gcaggatcgc ggtgtgcgcc tccgacggga
    14401 agcgcgccga cttgaagttg gtcggcagcc gcacctgcag cacgttcacc ttctcgtcgg
    14461 gcgggcgcga ggcttgcgga tcgggactgc acagcgtgac gtacgccgga tcgaagctgt
    14521 cccagaccgc cgcgtcggtg tagcagccgc ggcaggccgc ggcgtcgacg ccgggcacgc
    14581 gcgcgaggta cggcagcggc ggcatgttca tgatcgacat acagggcact ttcgccttcg
    14641 ccaccgcctc gagcagctcg cgcacgccgc tcgagcggta ctgcggctcc tgcatggcga
    14701 gcgccaccag gtcgaactcc gacgggttga tgccagcggg gccgccggcg gacagcttgc
    14761 cggtgagctt tcgcgagtcg acctcgacca ggccctcgcg ccccttgacc ggcatgcgta
    14821 cgcgcgcgcc ttcctggttg atcaggtccg cttccgcggg caggcacacg aggtgcgccg
    14881 tgtggccggc caggcaaagc ttggtggcga gcagcgatcc gtaggacgcg ccgaagatca
    14941 ggattttgta gggactaggc atagaatctc atgatgtgaa atcttcactc ggctgccgaa
    15001 aacaatacac ctatctagtt gatttatcat gttgcggcgc gacattccgc tacctagaat
    15061 gctcggatgc acatgtccag cccactcccc aagggcgtcg ccgtccgcgc cgccgtgacc
    15121 ccggcgttcg ccgagatcct gacgtcggaa gcactcgcgt tcgtcgccgc cctgcaccgc
    15181 gaattcgagc cgcgccgcca gcaactgctc gccgcgcgcg ccgcgcggca gaaggaattc
    15241 gatgccggca agctgccgga tttcctgccc gagacgcgca agacccgcga atccgactgg
    15301 aaggtcgcgc ctcaacccaa ggacatgctc gaccgtcgcg tcgagattac cggcccgacc
    15361 gaccgcaaga tggtgatcaa cgccctcaac tgcggcgcct cgaccttcat ggcggacttc
    15421 gaggacgcca attgcccgac gtggcacaac atgatcgacg gtcagatcaa tctgcgcgac
    15481 gcggtgcgcc gcacgatcag cttccagcag ggcgacaaga gctacgcgct gaacgaccag
    15541 actgccgtgc tcatcccgcg cccccgcggc tggcatctga acgagaagca cgtgacggtg
    15601 gacggcgagc aagtcgcggg cggcatcttc gatttcgcgc tgtacttctt ccacaacgcg
    15661 aacgagctgc tgcggcgcgg gagcgggccg tacttctacc tgcccaagct cgagtcgcat
    15721 ctcgaggcgc ggctctggaa tgacatcttc aaggcggcgc aggcggcgct cggcgtgccc
    15781 cagggcagca tcaaggcgac ggtcctgatc gagaccatcc tcgccgcctt cgagatggac
    15841 gagatcctgt acgagctgaa ggagcactcc gccggcctga acatcgggcg ctgggactac
    15901 atcttctcgt gcatcaagaa gttccgctcc aacgacaagt tctgcctcgc cgaccgctcg
    15961 caggtaacga tgacggcgcc gttcctgcgc gcctacgcgt tgttgctggt caagacctgc
    16021 caccggcgcg gcgcgcccgc gatgggcggc atggcggcgc agatcccgat caagaacgac
    16081 ccggccgcga acgaggcggc gctggagaag gtgcgccagg acaagctgcg cgaagtgacc
    16141 gacggctgcg acggcacctg ggtcgcgcat ccggggctgg tcccggtggc gaaggaggtt
    16201 ttcgaccggc acatgccgaa gcccaaccag tacgacaagc agcggccgga cgtgaacgtg
    16261 acggccaagg agctgctcga cttccgtccc gaggcgccga tcaccgaggc ggggctgcgc
    16321 aacaacatct cggtgggcat ccagtatctc ggctcctggc tcggcggcaa cggctgcgtg
    16381 ccggtgttca acctgatgga agacgcggcc acggccgaga tctcgcgctc gcagatctgg
    16441 caatggatcc gcagcgacaa gggcaagctc gaggacggcc ggcgcgtcac caagtcgctg
    16501 ttccggcatc tgctgtcgga ggagttgccc aaggtgcgcg cgtacgtcgg cgaggaagcg
    16561 tgggaagccg gcaactactc ggagagcgcg gcgctgttcg agaagatcac ttccgacgac
    16621 aactacgtcg agttcctcac gctgccggcc tacgagcgca tcaactgagc atcgggcgct
    16681 caggccacca gccagaccac gaacgaaacc agcgtcgcca ccacggcgat cgcgcccgcc
    16741 agcttgtacc acgggctcgc ttccatcttt tgcgctgccg cttgcaggcg tgcggactgc
    16801 agcgagcgct tccacgcggt cggcggctga tcgcccagga actgcgcgag ctcgcgaaag
    16861 aacatgtcgg ccgcgggttc atgcgccagg aacagatggt tgttgctctc gagcgtcacg
    16921 agcttagcgt cgggaatcgc cgcggcaagc tcccgcccct gatcgaacgg aacgcgccga
    16981 tcgccgcggc agtgaaggat cagcgtgggt acgcggactt gtggcagaag atccgtgatg
    17041 tcggtcgagg agttgacgag gaatgattgc tcggcgaatt cggcgctgga ggaggcgcgc
    17101 tcgagttctg aaaaccagcg caactgctcc ggcgtaccgt cgggcatgaa ttgagcgctg
    17161 aaaacctgtc ggaaagcggg atcatcgctg ccccagccgt cccggatcag gcggcgatac
    17221 gtttcgaccg cctcgggtcc ctggccggcg ccgctgcgcc ggctcgtgcc ccgcgcatag
    17281 gcgccgaaca ggatgaggtg gctcacgcgc tcaggatgtc ggaccgcata agcgatcgac
    17341 gctgctcccc cctgcgaaat gccgagcagc gggaatcgcg cgtgacctgc cgcctcgatc
    17401 acggtcgcga ggtcggtcac atagctttcg agcgataccg tggccggatc gcgatccgag
    17461 agcccggtcc cccgcatgtc gtagcggacg agcgtgcgcc gttcgctcag gccctcgagc
    17521 acatggcgcc agaccgggct ttgccattcg aactcgagat gcgtgaacca attccccgtc
    17581 ttgacgatcg gcgggccgtt gcccgaagtg gcatacgcca ggcgcacgcc atcgggcgtg
    17641 gtacagaact tgatcgattg ctgcagtggc ccctccctac ttgttcggcc gcgatcgcct
    17701 acgtcgcgcg accctcagga cctaagcgat ggtccactcg ttttcgcact tgcggcagct
    17761 cacggtcatc gagctgacgg tggcccgcac cagacggtag cggccatacg tcccgcagcg
    17821 cgtgcaggtc gagcgctcgc tcaggcgctg cgcctgcacc agcatgccca ggtaccgcca
    17881 gagcgcgtac caggcgaaca gcccggatac gcacgcggct accagcacca gcgctgcctt
    17941 gaagccgagc tcgagcaggc tcaggtcctc cagcagcgcg aacagcgcca ccgcgcccag
    18001 caggctcgcc accagccagc cgtggcacac gtacagctgg cgctcgtacc agcgccggaa
    18061 gccgagcttg ccgatcgcgc ccgccgggtc catgcaacga ttgtacggcc gcggcgctac
    18121 gcagtgcgca gctcggcgag ggccggcgcg tggtcggacg ggcgctcgag tttgcgcggc
    18181 gcctggtcca cgctggccgc ggtgcagcgc tcggcgagcg ccggcgacag caggatgtga
    18241 tcgatgcgca acccggcgtt gcggcggaat ccgagcatgc ggtagtccca ccaggtgtag
    18301 atcttctcgg gctgctcgaa caggcggaag ctgtccttca gccccagcgc gagcaggccg
    18361 cgccacgccg cgcgctctgg ctcggagaca tgcacctgac cctcccacgc cttggggtcg
    18421 tgcacgtccc ggtcttcggg tgcgacgttg aaatcgcccg ccaccgccag gcgcgggtgc
    18481 cgagcgaatt ctgcggcaag gtagtctctc agcgcggcaa accagcgcag tttgtagtcg
    18541 tacttttcag accccacggc ctgcccgttc gggcagtaga cgcacacgac gcgcacctcc
    18601 tgcacggtgg cggcgatgac gcgcctttgc tcgtcggcga atccggggat gtccggcgcg
    18661 atcccggcga ggggcgagcg cgcgagcagc gccaccccgt tgtaggtctt ctggccgttc
    18721 acagcgcagg catagccggc ggcctcgagc tcggcgcgcg ggaatttcgc ctcctcggcc
    18781 ttgagctcct gcaggcaaac gacgtcgggc cgcgctgtag cgagccactc gagcagctgc
    18841 ggcaggcgca ccttcaccga attgacgttc cacgtcgcga gcttcatgcc ggcgacgcgg
    18901 cgcgcgctag cgcggcgtgt acggtccggc cggcgcttcc tcggccggcg gcgcgtcgct
    18961 cgccgcagcg ggcttgggcg gcaccggctt cggcgcggcc tggttgcgcg gcggcagtat
    19021 cccggtgcgc ggatagcccg cgagatcgag catcgaatgg taggcgccct gctcgaagcc
    19081 cttctgcgtg ccggtgccga cgacgagcga gggcaccgac aggctgccca cggcttgacg
    19141 caactgcgcc tgctcctgct cgccgaccac gctcacctcg cgaaacggca cgccgcgctg
    19201 gttgagcagc gaacgggccg cgccgcaagg cgcgcaatca ggcgccgtaa acagcgtcac
    19261 cgggtagctc ttcgcggcga gctgcacggc gtagggttga tcggcgccgg attgcgctgc
    19321 gggcgccgtg tcggcggcgc gcttgcgcac gttcgtcgca gtgggcggcg gcggcgtgtc
    19381 ggtgacatgc acgcggccct tctcgtcggt ccagcggtag agctgcgcgc tggcggcgca
    19441 ggcagcgccg agcagtacgg caaagatcac gctgcgcatt cgcgatcctc ctgtttttcc
    19501 agcagtttcc cctacgcggg gatcatacca ttgtgccgca gcagcgcttc gacccgcggc
    19561 tcgcgtccgc ggaacgcgtg gaacgactcc gcggccggac ggctgccgcc gaccgccagc
    19621 acttggtcgc gaaagcgcgc accggtggcg gcatcgagga tctggggcgc ctcctcgaac
    19681 gcggcgtagg cgtcggccga gagcacctcg gcccacttgt agctgtagta gcccgcggcg
    19741 tagccgccgg caaagatgtg cgagaagctg ttcgcgaagc gattccacgc cggcggaatg
    19801 atcaccgcga tcttggcgcg cacgtcgcgc agcagatcga gcacgccgcg atcggcggga
    19861 tcgaaatccc agtgcaggcg catgtcgaac accgcgaact cgatctggcg cagcatcgcg
    19921 agcccggcct ggaagttctt cgccgccagc atcttgtcgt agagggaccg cggcagcggc
    19981 gcgccggtgt cgacgtggcg cgtcatgtgc tggagcacgt cccactccca gcagaagttc
    20041 tccatgaact ggctcggcag ctcgaccgcg tcccattcca cgccgttgat gcccgagacc
    20101 ccgaggtcgt ccacccgcgt gagcaggtga tgcaggccgt gtccgaactc gtggaacagc
    20161 gtcagcactt cgtcgtgcgt gaacagggcc gggcgagcgc cgaccgggcg cgagaagttg
    20221 cacaccacgt gcgccaccgg cgtctgcagc cgctcgtcca ggcgccggcg cgagcgcgcg
    20281 tcgtccatcc acgcgccgcc gcgcttggtc tcgcgcgcat acaggtccat gtagaactgg
    20341 ccgacgagct cgccgcgcgc atcgcgcacg tcgaaaaagc gcgcctcgga atgccatagc
    20401 ggggcgctgg cgctcgcgat gcgtacgccg tacagcgtct cgacaagacg aaacagccct
    20461 gccagcaccg cgtcctcggg gaagtactgc ttcacctcct gatccgagaa cgcgtagcgg
    20521 cgctcgcgca gccgctccga ggcgaagggg atatcccaca tctcgaggcg ctcgagcccg
    20581 agctcggtgc gcgcgaaggc ttcgagctcg gcgaggtcgc gctcggcgtg cgggcgcgcg
    20641 cgccgcgcca aatcctcgag gaactcgagc acctgcgccg gggactcggc catcttcggt
    20701 accagcgaca gctcggcgta gctcgggtag ccgagcatct gcgcggcttc gcggcgcaac
    20761 ggcagcaggc gcgcaatgag cgtcgtgttg tcgagttccg gcttgcccaa ctcggaggcg
    20821 cgcgtcgcgt aggcgcgata cagcgcctcg cgcagcgcgc ggtcctcggc gtactgcatt
    20881 accggcaggt aagagggcat gtgcagcgtg aacttccagc cgcgcccgtc ctcctgcttc
    20941 gccgattgca gcacgtcgcg cggaatgccg ccaaggcgcg ccgcgtcctc gatcaccagg
    21001 ctgaacgcgt tggtggcgtc cagcacgttc tcggagaact tcgcgcccag gcgcgcgatc
    21061 tcgtcctgga tctgcgcgta gcgtggcttc tgatcagcgg cgagctccgc gccgccaagg
    21121 cggaagtcgc gcaaggcgtt gtccacgatg cgccggcggg cgggggtcag tgcggcgaac
    21181 gcgggcgagt cgcgcaacgc tccgtacttc gcgaacagcg cctcgttgtg cccgagctcg
    21241 gtccagaact gcgtgagctt cggcagattt tcgttgtacg cggcgcgcag ctcggggctg
    21301 tcgagcacgg cgtgcaggtg cgatacctgc ccccaggcgc gccccaggcg ctcggtggcg
    21361 tcctcgagcg gcgcgacgaa ctcgtcccag tgcggcggcg cctgctcggc gcgcgccacg
    21421 gcggcgcgcg cttcggcgag cagcaggtcg atggcgggcg cgacgtgccc gggccggatg
    21481 tccgcgtagc gcggcaggcc ggagaattca agcagcgggt tattgccgag tctgtccaag
    21541 ggcgagctcc gtggcgcgta tcgtgtttgc gaccagcatg gccacggtca tcggcccgac
    21601 gccgcccggc accggtgtca gccagccggc gatcggcagg accgcggccg catcgacgtc
    21661 gcccaccagc ttgccgtccg caccgcgatg aatgcccacg tcgacgacgc aggctccggg
    21721 cttgaccatc tccgcgccca cgaggcgcgg cacgcccgcg gcggcgacca gcacgtcggc
    21781 ctcgcgcgcc agcgcggcca agtgctcggt cttggagtgg cagatggtga cggtggcatc
    21841 cttttgcaag agcagcagcg cgagcggctt gccgacgatg ctggagcggc cgatgaccac
    21901 ggcgcgccgt cccgccagcg ccacaccggc atgctcgagc aggcgcagga tgccggccgg
    21961 agtgcagggc aggaagcgcg gcgtgccgcg cagcagcagg ccgaggttgt cggcgtgaaa
    22021 gccgtccacg tctttggtcg gcgagacggc cgccagcacg cgctcggcgt ccacgccgcg
    22081 gggcagcggc agctgcacga ggatgccgtg cacgcgcgga tcggcgttca gcgccgcgac
    22141 gcgctccagg atctcgcgct cggttgcccg tgcgggcagc tcgtgcgact ccgagcgcac
    22201 gccggtctcc tcgcaggcgc ggaccttgtt tcgcacatac acgcgcgacg cagcgtcgtc
    22261 gccgacgacg atcgcggcca gcccggggcg catgccgcgc gcggcgagct gctgcaccgc
    22321 cggcttgagc gcggtgcgta gctcggcgga taacgccttg ccgtcgatga tcttcgcagc
    22381 catgtgttgc ataatacgac gacccgaagc gagccctggc agcagtccct gcggccacaa
    22441 gctggagtaa gcgatgtccg ccgttcccct acaccctgcc gcgaacgaca gcgatacgct
    22501 cgagacccag gagtggctcg atgcgctgga ggccgtgctc gagcgcgagg gccccgagcg
    22561 cgcgcacttc ctgctggagc gcctggtgga aaaggcgcgc accagcggcg cctacattcc
    22621 gttctccgcg aacaccgcct acctcaacac catcccgccg cacctcgagg agcgctcgcc
    22681 gggcgacgtg accctcgagg agcgcatccg ctcgatctgc cgctggaacg cgatggtgat
    22741 ggtggtgcgc gcgaacaaga gcgacgacga gctcggcggc catatcgcga gctttgcgtc
    22801 ggtggggacg ctgttcggca ccggccagca gcatttctgg cacgcgccgc acgaaggcca
    22861 cggcggggac ctggtgtact tccagggcca ttcctcgacc ggcgtgtacg cgcgcgggct
    22921 gctcgagggc cggctgaccg aggagcagct caacaacttc cggcgcgaag tcgacggcaa
    22981 gggcgtgtcg tcctacccgc acccgtggct gatgcccgat tactggcagt tcccgaccgt
    23041 ctcgatgggc ctcgggccga tccaggcgat ctacatggcg cgctatctca agtacctgca
    23101 cgcgcgcggc ttcgcggaca cggccaaccg caaggtgtgg gtgttctgcg gcgacggcga
    23161 gatggacgag cccgagtcgc tcggcgcgat cggcatggcg gcgcgcgaga agctcgacaa
    23221 cctcgtcttc atcgtcaact gcaacctgca gcgcctggac gggccggtgc gcggcaacgg
    23281 caagatcatc caggagctgg aaggcgattt ccgcggcgcc ggctggaacg tgatcaaggt
    23341 gatctggggc tcgtactggg atccgctgtt cgcgcgcgac accgaagggc ggctgctgcg
    23401 cgtgatggaa gagacggtgg acggggagta ccagaactac aaggccaacg acggcgcctt
    23461 cgtgcgcaag cacttcttcg gcaaggatcc gaaggtgctg gagctcgtgt cgcgcatgac
    23521 cgacgaggac atctggcgcc tgaaccgcgg cggccacgac ccgcacaaga tctacgccgc
    23581 ctacgccgcg gccgtgaagc acaggggcca gcccacggtg attctcgcca agaccatcaa
    23641 gggctacggc ctcgggaagc agggacaggc gaagaatccg acgcaccagc tgaagaaggt
    23701 cgacacggcg accatcaagg agatgcgcga cagcttcaac attccgatcc cggacgacag
    23761 gctcgacgag ttgccgttct acgtgccgcc gccggattcg ccggagatgc agtacctgca
    23821 cgagcgcagg aagaagctcg gtggcttcct gccgcagcgg cgccgcaagg cggaccagtc
    23881 gccggcggtg ccgccgctct cggcattcga cgcggtgctc aaaggctcgg gggagggtcg
    23941 cgagatctcg accaccatgg cgttcgtgcg gctgctcacc gcgctggtgc gcgacaagga
    24001 gctcggcaag cgcatcgtgc cgatcgtgcc ggacgaggcg cgcaccttcg gcatggaggg
    24061 catgttccgg cagctcggca tcttcgcgcc cgagggccag aagtacgagc cggtcgacaa
    24121 ggaccaggtg atgtactacc gcgaggacaa ggcggggcag atcctggaag agggcatcaa
    24181 cgaggcgggc gccttctcgt cgtggatcgc cgcggcgacc tcctacagcc actccaacgt
    24241 ggtgacggtg ccgttctaca tcttctattc gatgttcggc atgcagcgga tcggcgactt
    24301 ggcatgggcg gcggcggacc agcgcgcgcg cggcttcctg ctgggcgcga cggccgggcg
    24361 cacgacgctg aacggcgaag gcctgcagca cgaggacggg cattcgcacg tgctctcctc
    24421 ggtgatcccg aactgcgtgt cctacgaccc gacctacggc tacgagctcg cggtgatcgt
    24481 gcacgacggt ctgcgccgca tggtcaccga gcaggaggac gtcttttact acctgacggt
    24541 gatgaacgag aactacgagc acccgccgat cccgaagggc gcggaggacg gcatcctgaa
    24601 gggcatgtat ctgctgcgcg agtcgaaatc gaaggccaag acgcgcgtgc agctcatggg
    24661 ctcgggcacc atcctgcgcg aggtcgaggc cgccgccggg ctgctggaga aggactgggg
    24721 cgtggccgcc gacgtctgga gcgcaacgag cttcaccgag ctgcggcgcg acggcctcgc
    24781 cgccgagcgc tggaacctgc tgcatcccgg cgacaagccg cgcctggcct acgtgacgca
    24841 gatgctcgag aagcgcccgg cgggaccggt ggtggcggcg agcgactaca tcaagacctt
    24901 cgccgaccag atccggcctt tcgtgccgcg cgagcgcgtg taccgcgttc tcggcaccga
    24961 cggcttcggg cgctccgact cgcgcgcgaa gctcaggcac ttcttcgaag tcgaccgcca
    25021 tttcgtcgcc gtcgcggcgc tgcaggccct tgccgagcag ggcgacggca aggcgaaggc
    25081 agtggccgac gccctgaaga agtaccgcat cgaccccgat aagcccgatc cgaccacggt
    25141 atgagagaaa tcagcgtccc ggacattggc gatttcaagg acgtcgaggt gatcgaggtc
    25201 ctggtgaagg ccggcgacac gatcgccgcc gagcagtcgc tcatcaccgt ggagtccgac
    25261 aaggcgacca tggagatccc ggcgcccggc gccggcacgg tgaaggaaat gcgcgtcaag
    25321 gttggcgaca aggtgtcgca aggcacgccg atcctggtgc tggacgaggc gaaaggcgat
    25381 gctgagccca aggccgcgcc agcccctgcg ccgcagaagg cgcaggaagc accagcgccg
    25441 gcgccggaga aggcggcggt cgtgcccgcg gccaagagcg ccgggccgat gacggtgaag
    25501 gtgcccgaca tcggcgactt caaggatgtc gccgtgatcg aggtgctggt gaaacccggc
    25561 gacacggtgg cgcatgagca gtcgctgatc accgtggaat cggacaaggc gacgatggag
    25621 atcccgtcgc ccgctgccgg caagatcacg gcgctgaagc tcaaggtcgg cgacaaggtt
    25681 tcggaaggct cgacgattct cgagctcgag gccgagggcg ccgcccccgc ggcgcccact
    25741 gcaaagccga ccaaggcgca cccgacgccg gcggcggtgc ccgaagccgc ggcgccgcat
    25801 cgtccgccgg tgccgaagga gccgcaggaa gccacgcacg ccaagccgca cgcgagcccg
    25861 tcggtgcgca agttcgcgcg cgagctgggc gtcgacctca cgcgcgtgaa gggcagcgga
    25921 ccgaaagggc gcatcgtgca cggtgacgtg caggcgttcg tgaaaggcgc gctcgccgcg
    25981 gtgcccgcgg cgccggcggc cgggggcggc gcgcagccgt tcaacctgcc cgcctggccg
    26041 gacgtggact tcgccaagtt cgggcccgtc gaggcgaagc ccctgacgcg gctgcagaag
    26101 ctctccggcc cgaacctgca ccgcaactgg gtctcgatcc cccacgtcac gcagttcgac
    26161 gaggccgaca tcaccgagct ggaggcgttc cgcaagtcga acaccgccga gaccgagaag
    26221 cagggcttca agctcaccat gctcgccttc ctcgtcaagg cctgcgtgac cgcgctgcgc
    26281 cagcacccga acctgaacgc gagcctcgat cgcggcggcg agaacctgat cctgaagaag
    26341 tactacaaca tcggcgttgc cgtggacacg cccgacgggc tcgtcgtgcc ggtggtgcgc
    26401 gacgccgaca gaaagggcgt gttcgacgtg gcgcgcgagc tcggcgagat ctcgaagctc
    26461 gcgcgcgaca agaagctcaa gcccggcgac atgcaaggcg gcacgttctc gatctccagc
    26521 ctcggcggca tcggcggcac ggcgttcacg ccgatcatca acgcgcccga ggtggcgatt
    26581 ctcggcgtct cccgttcggt gctgcgcccg gtgtggagcg gcaaggagtt cgcgccgcgc
    26641 ctgatgctgc cgctgtcgct gtcgtacgac catcgcgtga tcgatggcgc ggcggcggcg
    26701 cgcttcacgt cctacctcgc gagcgtgctc gtggacatcc gccggtcgct gctgtagatg
    26761 gccgagctcg actgcgacgt cgtggtactg ggcgcgggac ccggcggcta ctccgccgcg
    26821 tttcgcgccg ccgacctcgg cttgaagacg gtgctggtgg agcgctaccc gacgctgggc
    26881 ggcgtgtgcc tcaacgtcgg ctgcattccg tcgaaggcgc tgctgcatac cgcggcggtg
    26941 atggatgcgg cgcaggcgct ggcgagccac ggcatcagct tcggcgcgcc gcaggtcgac
    27001 ctcgacaagc tgcgcgcgtt caaggaaaag gtggtcggca agctcaccgg cggcctcgcc
    27061 gcgatggcca agatgcgcaa ggtgaccgtg gtgcagggcg tgggtacgtt cagcggcgcg
    27121 aatcagctcg ccgtcgaggg tacggacagg accacggtgc gcttcaagag cgcgatcatc
    27181 gccgcgggct cgcaatcggc gaagctgccg ttcctgcccg atgacccgcg catcgtggat
    27241 tccaccggcg cgctcgagct caagagcgcg cccaagaagc tgctcatcat cggcggcggc
    27301 atcatcggcc tcgagatggg cacggtgtac tccacgctcg gcgcgcggct cgacgtcgtc
    27361 gagatgatgg acgggctgat gctgggcgcg gaccgcgacc tggtggcggt gtggcagaag
    27421 ttcaacgcca agcgcttcga ccgggtgatg ctgaagtcga agaccaccaa ggcggaagcc
    27481 accaaggcgg gcatccgcgt ctctttcgag gggcaggagg cgcagaccta cgacctggtc
    27541 ctggtctccg tgggccgggt gccgaacggc aacaagatcg gcgccgacaa ggccggtgtc
    27601 cgggtcgacg agcgcggctt catcgcggtg gactcgcaga tgcgcaccaa cgtgccgcac
    27661 atctacgcca tcggcgacat cgccggcaat ccgatgctcg cgcacaaagc ggtgcacgag
    27721 gcgcacgtcg cggccgaagc cgccgccggc cagaagtcgc acttcgacgc gcgcgtggtg
    27781 ccctcggtgg cctataccga ccccgagatc gcgtgggtgg gcctcaccga ggacgaggcg
    27841 aagaaggcgg gcaccaagat cggcgtggcg aagtttccgt ggtcggcttc gggccgcgcc
    27901 atcgccaacg cccgcgacga gggcttcacc aagctgatct tcgacgccga atcgcaccgc
    27961 atcctcggcg gcggcatcgt cggcacgggg gcgggcgacc tgatcagtga gttggcgctg
    28021 gcggtggaga tgggtgccga cgccaccgac atcggcaaga ccatccatcc gcaccccacg
    28081 gtgtccgagt cgatcggcat ggcggcggaa gccttcgagg gcgtctgcac cgacttgccg
    28141 ccgcagagga agaaatgacc caggacgaat tgaagcagct tgtcgcgcgc gaagcgctga
    28201 agcacgtggt cgaggacgcc gtggtcggcg tcggctcggg ctcgacggtc aacttcttca
    28261 tcgatgcgct tgccggcatc aagggccgca tcgaaggcgc ggtggcggcc tccgatgcga
    28321 gcgccgagcg actgaagaag cacggcatcc gcgtcttcga cctgaacgcg gtcgacgagc
    28381 tgccggtgta cgtggacggc gccgacgagg tgaacgagca cctgtacatg atcaagggcg
    28441 gcggcggcgc cctcacgcgc gagaagatca tcgccgccgt ggcgcggaaa ttcgtgtgca
    28501 tctgcgatgc ttccaagctc gtcggcgtgc tgggcaagtt cccgctgccg gtcgaggtga
    28561 tcccgatggc gcgcggacac gtcgggcgcg agttgcgccg gctcggcggt caccccgagc
    28621 tgcgcgaagg cttcaagacc gacaacggca acctggtcat cgattgccgt ggcctgacca
    28681 tcctcgaccc gccgcggctg gaagccgagc tcaacaacat cgccggcgtg gtcaccaacg
    28741 gcctgttcgc gcggcggccg gccgacctgc tgctgctggc cgaggccggc gcggtgcgtg
    28801 ccgtggtcgc cccgaaggcg taacattccc gtgatattcg cgcgataggt tgagcgacga
    28861 tgcccgagca cacctccaag caattcgacg ccgagctgga gagcgtgcgc tcgcgcgtgc
    28921 tgcagatggg cggcctggtc gaggagcaga tcatccgcgc catggacgtg ctggcggcgg
    28981 gcgacatgca gcagatcgag aaagtgatcg ccgacgatca ccgcgtcaac gccatggaag
    29041 tcgagctcga cgaactgtgc agccacatca tcgcgcgccg tcagccggcg gccgtcgacc
    29101 tgcggctcct gatcaccgtc atcaagacca tcaccgacct cgagcggatc ggggacgagg
    29161 cggagaagat cggccgcatg gcgaagctca tccacgccgc ggagcgccgg cacatgccgc
    29221 gcctggacct gtcgccggtc gccgagcgcg cccttgccat gctgcgccag tcgctcgatg
    29281 ctttcgcgcg cctggacgtc tcggaggcga tgcaggtggt aaagcaggac cacgaggtgg
    29341 acgacgcctt ccgcgccatc atgcgccagc tgatcacctt catgatggaa gacccacgca
    29401 ccatcacgcg ctgcctcgag gtgctgttcg tcgccaaggc gatcgagcgc atcggcgacc
    29461 atgccaagaa catggccgag tacgtgatct acatggtgaa agggcgcgac gtgcgccaca
    29521 cctctatcga agaggtggaa aaggaaatac tgcggtaagt agggacagac ctcattttcc
    29581 cggctgcgca ccaggcgcaa gcgaggaaaa tgaggtctgt ccctatttaa ttgctcggcg
    29641 gaacgtaccc cgaagcgacg tcggcgccct cgccgaagaa atgcttctcc atctgctcgg
    29701 cgaggtactt gcgcgcgcgg atgtcggcca ggttcagccg gttttcgttc accagcatgg
    29761 tctggtgcct cagccactgc gcccaggcct ccttcgacac gttctcgaag atgcgcttgc
    29821 cgagctcgcc cgggtagggc ggaaagtcga gcccctcggc ctcgcggccg agcttgatgc
    29881 atttcaccgt tctagccatg acgtggacgt tccgattcag attcgcttga ggaacacttt
    29941 ggagctgcgc ctccaattat agagcgactg gcgttcttcg ggcagcgccg cgggcgacgc
    30001 gggcttgaag ccctgctcga gaaaccagtg ctcggcgcgc gtggtgagcg catagaggcg
    30061 ccggatcttc tgctgccggg cgcgcgtctc gcaggcgttc agcagcagct cgccgtggcc
    30121 ggcgtcgcgc gcgccgggcg ccacggcgag gcaggcgagc tcgccgctct tcgcgggcag
    30181 cgggtacagc gcagcgcagc caagcagcgc gccgtcgtgc tcgagcacga tgaaattgcc
    30241 gatttcgcgc tccaggcgct cgcggccgcg cttgaccaga gtgccgtcgg cttcgagcgg
    30301 ctc
//
LOCUS       DBNHKE010000111        15101 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_811984, whole genome shotgun sequence.
ACCESSION   DBNHKE010000111 DBNHKE010000000
VERSION     DBNHKE010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_811984"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="AC16O9_06930"
     CDS             complement(<1..137)
                     /locus_tag="AC16O9_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HIR6516386.1"
                     /translation="MRASSHRPRKRFGQHFLHDATVVRRIVEAVAPSADDFIVEIGPG
                     EG"
     gene            complement(109..1014)
                     /gene="pdxA"
                     /locus_tag="AC16O9_06935"
     CDS             complement(109..1014)
                     /gene="pdxA"
                     /locus_tag="AC16O9_06935"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6516387.1"
                     /translation="MSVATIALTSGEPAGIGPELCVAAARVFRGARLVAIGDRSLLRG
                     CANIEHVPLAAPRRAGRLDPANSRYVLALLDRAAAGCLAGEYDAMVTAPVHKGAINDA
                     GIAFTGHTEYLAAKAGAPHVVMMLVGGGLRVALATTHLPLAAVPRAITRDGLLATLRV
                     VHAGLQKRFGIRRPRILVAGLNPHSGESGHLGTEERDTIEPAIAAARKLGIAAEGPIP
                     ADTLFVPARLKAADCALALYHDQGLPVLKYASFGRGVNVTLGLPFVRTSVDHGTALDI
                     AGKGRADAGSLLEALKLARKLASAA"
     gene            complement(1011..2306)
                     /locus_tag="AC16O9_06940"
     CDS             complement(1011..2306)
                     /locus_tag="AC16O9_06940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6516388.1"
                     /translation="MTRPAAGLLLALAALVPAAAAARDAVLVDRVVAVVNKDVITLTE
                     LAQRTERAAAELRRQRIAAPERGVLQRQVLERLVLDKAQLQLAESTGLRVDELQLDRA
                     VQRVAQNNGMALAEFRRVLERDGIDFEEFRAELRQQILLARLRDREVEDRVQVSEAEI
                     DAFLEDNKAGLADAVEYDAAHILVRVPEQASPEQVAQARARAARVREEARAGADFARL
                     AASHSDAGDALQGGALGWRTPGRLPELFAEALRDMQPGTVSELLRSPAGFHVVKLLGR
                     RGAGAAAAVTQTRARHILVKTNEIVSEEDARRRLGGLRERISAGADFAELARLNSEDG
                     TAARGGELGWVYPGDTVPEFERAMNALGPGELSPPVRTPFGFHLIQVLERRAADVSAE
                     RQRLQARQALRERKADEAYQDWLRQLRDRTYVELRLEER"
     gene            complement(2307..4547)
                     /locus_tag="AC16O9_06945"
     CDS             complement(2307..4547)
                     /locus_tag="AC16O9_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR6516389.1"
                     /translation="MQRFPARKLATQVAGCASLLAACAAFAQMPAPEAREPATIDAQS
                     IEGISGLEVTARGGVEFRQGESQIYAEYLKFNREFGRLEADGGVRVAQGTDRLFGPRL
                     IYDTTDDTGVMEAPTFLMRRKQTARGGAERIEFRGRGRYLFSGAHFTTCEPGNDDWRL
                     EASELELDYTEETGVARGARLRFFDVPILGTPYFSFPLENRRKSGLLPPHYAQTTLRG
                     GELTVPFYWNIAPEQDATLTPTFMSKRGEQLKTLYRYLGRSFNGEARLEYLPEDKLLG
                     RSRTGYSVQHEHRFTPALTAKVDVNHVSDDRYFVDLYSDVRQVATGNLQRDGYLQYGR
                     SFGGTGLSLQGRVQRFQTLQDPLAPIVPPYHRVPQLNLSVARNDMGGLLDGALPIEYA
                     RFTHPTLVEGERSTVNPVLSMPLTAPGYFLTPRFGARHSRYELSRTAAGQPERQTVSI
                     PWLSLDAGLLFDRGTDWFGERLTQTLEPRLFYVYAPFREQSQLPLFDTALADFNYAQL
                     FSENRFVGGDRFGDANQITWALTSRLLNERGEERLRATLGQRYYFAEERVGLTASSTL
                     RTSTESDWLASAGGRFARHWTFDGALQYNPRNSRTERYGLQLRYAPEIAKVLNFSYRF
                     NREQVRQWDLSAQWPVQGGWYAIGRYNYSLRDRRLLEALGGIEYNGGCWVFRAVAQRV
                     QAATDVTSKAILFQLEFNGLGQIGSSETVNLFRRNVPGYSVTNPRDQTLVPPSLQPAA
                     PFEGAN"
     gene            4617..5588
                     /locus_tag="AC16O9_06950"
     CDS             4617..5588
                     /locus_tag="AC16O9_06950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6516390.1"
                     /translation="MDHRLAALTRWLAGPLGGPEFTLSVASADASFRRYFRVTRADGR
                     SLIAMDAPPEHEDCAAFVHVARLLRAAGVNAPEVHAEDLAQGFLLLTDLGTVTYLDAL
                     NDANADALMRDAIAALLKWQQATRDATLPPYDEALLRRELALFPDWYVGRHLKRELSA
                     AQRAQWEALCAALVASALAQPPVYVHRDYMPRNLMLSEPNPGVLDFQDAVIGPITYDI
                     ASLTRDAFLSWDEARVLDWSVRYWEGAKRAGLPVAADFAEHWRAQEWMGLQRHLKVLG
                     IFARLTYRDGKARYVADAPRFLGYARAAARRYVALAPLARLLDELHA"
     gene            5585..6268
                     /gene="murU"
                     /locus_tag="AC16O9_06955"
     CDS             5585..6268
                     /gene="murU"
                     /locus_tag="AC16O9_06955"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HIR6516391.1"
                     /translation="MKAMVLAAGLGTRLRPLTDAVPKALVEAGGKPLIAWQLERLARA
                     GFREVVINVSWLGERIEARLGDGRAFGLALAYSREAEPLESAGGIAQARELLGPAPFA
                     LVNSDVFCDFDLAALRRRALGAHLAHLVLTPNPPHHPAGDFSLEAGAAGNAAAPRYTY
                     TGIALIDPALVAPVRSGERAQLAPLLRAAAAAGRLSGELHAGAWHDVGTAERLAELEA
                     RLAETGERT"
     gene            6265..7593
                     /locus_tag="AC16O9_06960"
     CDS             6265..7593
                     /locus_tag="AC16O9_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR6516392.1"
                     /translation="MKANDLKADVAVHAERRRRLAAALGEGVAVIPTAPERARNRDSH
                     YPYRHDSYFYYLTGFQEPEAVLVLAGGAEPRALLYCREKNPERETWDGFRHGPENARE
                     RYGFDEAHPVAALDEAMPALLADRSALWYPVGADAEWDARAMRWLNAVRAKARAGVAA
                     PQTLHDVRAPLDEMRLVKDAHELAVMRRAAAISAAAHRRAMLAARPGRREYEVEAELL
                     HEFRVNGAQFPAYTPIVAGGANACVLHYVQNDAVLADGTLLLIDAGCELEGYAADITR
                     TFPVNGRFSGAQREVYEIVLAAQRAAMGKVRAGNAWNEPHDAAVRVLAQGMLDLKLLA
                     GSLDEALEKETYKRFYMHRTGHWLGMDVHDAGEYKRAGAWRRLEPGMTLTVEPGLYIR
                     AADDVPERLRDIGVRIEDDVHVTAAECEVLTAAAPKAMAEIEALIRDGRR"
     gene            7577..8626
                     /locus_tag="AC16O9_06965"
     CDS             7577..8626
                     /locus_tag="AC16O9_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6516393.1"
                     /translation="MTAGADILVRGAGPVGCTLALALRGSRHAVRLVDPQPAVRAAFR
                     PIALSHASRLILERAGVWGDFPVTPIMSIRVSQAGAFGRTRMDAADAGVPALGYVTQY
                     AELLAALRARLAIDADDGTAARCTVHAEGTAADLRERRYAHDAVVARVAAEPAPRAEA
                     FERFTAEGPLALLPLAGSWALIWSARAQRAAALAAAAPQEFLALLQAACGERAGRFTA
                     VHERAQVPLALRVRASRVKGREVYIGNAAQTLHPVAGQGLNLGLRDAWDLAQALHEAE
                     DAGDPGVLARFAALRRVDAGATIGATEFLARAFLGAHPLARAARGLALTSLDILPPAR
                     RFFARRMIFGPSALP"
     gene            8676..9644
                     /gene="dusB"
                     /locus_tag="AC16O9_06970"
     CDS             8676..9644
                     /gene="dusB"
                     /locus_tag="AC16O9_06970"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR6516394.1"
                     /translation="MQLGAHVLRNALFAAPMAGVTDRPFRILARRYGAGLATSEMVSS
                     RPELRGSRKSRLRVDHAGEPGPVSVQIAGAEPAMMAAAAVYNVERGAQIIDINMGCPA
                     KKVCNVYAGSALLEDEALVARILEAVVAAVDVPVTLKIRTGPAPVRRNAVRIAKIAED
                     CGVQLLAVHGRTRACGFAGHAEYDTIGEVKASVKLPVIANGDIETPQQAHAVLERTGA
                     DGLMIGRAAFGRPWIFREIEHFLATGATLAPPGPAEIEAVVREHLEGLYGLYGEEQGL
                     RVARKHLGWYTKALPEGERFRQAVVRLQAAATQRAAVNDYFARLAA"
     gene            9641..9880
                     /locus_tag="AC16O9_06975"
     CDS             9641..9880
                     /locus_tag="AC16O9_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6516395.1"
                     /translation="MSRGSELGDAVRRSLERYFKDMDGEHPTAIYDMVLKHVEKPMIE
                     LVLGRAEGNQSRAAAMLGIDRNTLRKKMQQLRIKG"
     gene            9877..11436
                     /gene="purH"
                     /locus_tag="AC16O9_06980"
     CDS             9877..11436
                     /gene="purH"
                     /locus_tag="AC16O9_06980"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6516396.1"
                     /translation="MSKLKRALISVSDKSGIAEFARALAGLGVEILSTGGTAKLLEKA
                     GVAATEVSAHTGFPEMLDGRVKTRHPKIHGGILARRDSAAHVAAIEQAGIAPIDLVVV
                     NLYPFQATVADPDSRLEDAIENIDIGGPAMLRSAAKNHAGVAVVVDPADYARVLEELR
                     ATGEVSAPTRFALATKAFAHTAAYDGAIANYLSSLDAKGARGAFPGVLSLQFAKLQDM
                     RYGENPHQAAAFYRDTAPQPGGIAGYRQLQGKALSYNNVADADAAWECVKSFGEPACV
                     IVKHANPCGVAIARSALEAYRKAFKTDPTSAFGGIIAFNRALDAAAAAQIGRQFAEVI
                     IAPRVDAAARAAFAGKANLRVLEVPLARAPNALDLKRVGGGLLVQSADAHELAAGDLK
                     VVTRRKPGKAQLADLLFAWRVAKFVKSNAIVFVRGGMTVGVGAGQMSRIDSARIAAIK
                     AKAAGLTLKGSVAASDAFFPFRDGLDAVAKAGAVAVIQPGGSVRDDEVIAAADARKVA
                     MVFTGVRHFRH"
     gene            11501..12742
                     /gene="purD"
                     /locus_tag="AC16O9_06985"
     CDS             11501..12742
                     /gene="purD"
                     /locus_tag="AC16O9_06985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR6516397.1"
                     /translation="MKLLVIGSGGREHALAWKLSQSPRVQKVYVAPGNGGTAAEPDAE
                     NVPLSAVDELVHFAKNEGVYLTVVGPEAPLAAGVVDAFREAGLRIFGPTRAAAQLESS
                     KDFAKRFMARHGIPTARYATFEDAAAAKAYVEKEGAPIVVKADGLASGKGVVVAQSVA
                     EAHAAIDRLGGKLVIEQCLQGEEASFIVIADGAHVLALATSQDHKRLRDGDTGPNTGG
                     MGAYSPAPVVTPKVHARVMREIIRPAIAGMAKDGTPYTGFLYAGLMIDASGAPKTLEF
                     NCRLGDPEAQPILLRLKSDLLALLDAALEGRLENAEAEWDRRAALGVVLAAHGYPEAP
                     RQGDRIEGIPAPADDCRVFQAGTRTQGKDLVASGGRVLCVTALGDNLRAARSRAYAAV
                     ERIRFDGMQYRKDIGHRALHR"
     gene            12739..13608
                     /gene="hemF"
                     /locus_tag="AC16O9_06990"
     CDS             12739..13608
                     /gene="hemF"
                     /locus_tag="AC16O9_06990"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIR6516398.1"
                     /translation="MNLAAVSDYFLGLQGAIVARLEALDGGKFREDRWSRPEGGGGRA
                     CVIEDGALLERGGVNFSSVQGARLPPSASATRPQLAGRAFEAMGVSLVLHPRNPYCPT
                     VHMNVRCFAAGDVWWFGGGMDLTPYYGFEEDARHFHATCKAAVGARYPGFKRWCDEYF
                     YLKHRQEPRGIGGIFFDDLNEPDFDACFALVRSVGDHFLTAYAPIVEKRRQTPYGERE
                     REFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTEAILMSLPPRVAWRYDWVPAAGSPEA
                     RLYGEFLKPRDWV"
     gene            complement(13605..14789)
                     /locus_tag="AC16O9_06995"
     CDS             complement(13605..14789)
                     /locus_tag="AC16O9_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR6516399.1"
                     /translation="MKGLFWLLAVFAAAAALAVFGHLERGYVQFAYAEWRVEMSLLLY
                     AVLALLAFIGAFIAVRMLQHTVALPAYVRAYRARRRRERAQAALASALQAWLEGRYAR
                     AEKEATRAFDGAASPGLAAIIAARAAHELGAPERREQWMTRAQEAGQRGAAMLSRAVM
                     ALGERDYGSAADALHSLQGSGARQFATLRLLLRAERGLRNWEEVLRLTQQLAKRDAIA
                     PGVAEEYRVQAHVELLAQAAADRGTFEERWRKIPSREQSIARIAGAAARHALALGLTN
                     LARDAVEKALAKEWAPALAALYGELPGMEQPARGEEARRRIERAEKWLLEHESDPQLL
                     AALGRLCAHAELWGKAQNYLEASLSFEESRATHLELARLLDRLERSAEAQPHYRRAAE
                     LP"
     gene            complement(14786..>15101)
                     /locus_tag="AC16O9_07000"
     CDS             complement(14786..>15101)
                     /locus_tag="AC16O9_07000"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR6516400.1"
                     /translation="RVDAPQPPLLPPEQAYFLRENLRLRLLNARLALLSRNQAGFEQD
                     VGAARRWLLRYYDARARSTQEALAQLEQLQGSPISFEPPSITDSLDAVRNFKARRDRA
                     AG"
BASE COUNT         2113 a         5452 c         5451 g         2085 t
ORIGIN      
        1 ccctcgccgg ggccgatctc gacgatgaaa tcgtccgcgg acggcgccac cgcctcgacg
       61 atgcgccgca ccaccgtggc gtcgtgcagg aaatgctggc cgaacctctt acgcggccga
      121 tgcgagcttg cgcgcaagct tgagcgcctc gagcaggctg ccggcgtcgg cccgcccctt
      181 gccggcgata tcgagcgcag tgccatggtc gaccgaagtg cgcacgaacg gcaggccgag
      241 cgtgacgttg acgccgcggc cgaagcttgc gtacttcagc accggcaagc cctggtcgtg
      301 atacagcgcg agcgcgcagt cggcggcttt gagccgcgcc ggcacgaaca gcgtatcggc
      361 cgggatcggc ccctcggccg cgatgccgag cttgcgcgcc gcggcgatgg ccggctcgat
      421 cgtgtcgcgc tcctcggtgc cgaggtgacc cgactcgccg ctgtgcggat tgaggccggc
      481 gaccaggatg cgcggacggc ggatgccgaa gcgcttttgc aggccggcgt gcacgacgcg
      541 cagcgtcgcg agcagcccgt cgcgcgtgat ggcgcgcggc accgccgcga gcggcaggtg
      601 ggtggtggcg agcgcgaccc gcaggccccc gcccaccagc atcatcacca cgtgcggtgc
      661 gcccgccttc gccgccaggt actcggtgtg cccggtgaac gcgatgccgg cgtcgttgat
      721 cgcgcccttg tgcaccggcg ccgtcaccat cgcgtcgtac tcgccggcga ggcacccggc
      781 cgccgcgcga tcgaggagcg ccagcacgta gcggctgttc gccgggtcga gccgccccgc
      841 cctgcgcggc gccgcgagcg gcacgtgctc gatgttcgcg cagccccgca gcagcgagcg
      901 gtcgccgatc gccacgagcc gcgcgccgcg gaaaacgcgc gccgccgcga cgcacagctc
      961 cgggccgatg cccgccggct cgcccgacgt gagggcgatc gtcgccacgc tcagcgctct
     1021 tcgagccgca gctcgacgta ggtgcggtcg cgaagctggc gcagccagtc ctggtaggcc
     1081 tcgtcggcct ttctttcgcg cagcgcctgg cgcgcctgca ggcgctggcg ctcggcgctc
     1141 acgtcggcgg cgcgccgctc cagcacctgg atcaggtgga agccgaacgg cgtgcgtacc
     1201 ggcgggctga gctcgccggg gccgagcgcg ttcatggcgc gctcgaactc gggcaccgtg
     1261 tcgccgggat acacccagcc gagctcgccg ccgcgcgccg cggtgccatc ctcggaattg
     1321 agccgcgcga gctcggcgaa atcggccccc gccgagatgc gctcgcgcag gccccccagg
     1381 cgccggcgcg cgtcctcctc ggacacgatc tcgttggtct tgaccaggat gtggcgcgcg
     1441 cgcgtctgcg tcaccgccgc ggcggcgccc gcgccgcgcc gtccgagcag cttgacgacg
     1501 tgaaagcccg ccgggctgcg caacagctcg ctcaccgtgc ccggctgcat gtcgcgcagc
     1561 gcctcggcga acagttccgg cagccgcccc ggggtgcgcc agcccagcgc gccgccctgc
     1621 agcgcgtcgc cggcgtccga atggctggcg gcgaggcgcg cgaagtcggc gcccgcacgc
     1681 gcctcctcgc gcacgcgcgc ggcgcgcgcg cgggcctgcg ccacctgctc ggggctcgcc
     1741 tgctccggca cgcgcaccag gatgtgcgcc gcgtcgtatt cgaccgcatc cgccaggccc
     1801 gccttgttgt cttccaggaa ggcgtcgatc tcggcctcgc tcacctgcac gcggtcctcc
     1861 acctcgcggt cgcgcaggcg tgcgagcagg atctgctggc gcagctcggc gcggaactcc
     1921 tcgaagtcga tgccgtcgcg ctccagcacg cggcggaact cggccagcgc catgccgttg
     1981 ttctgcgcga cgcgctgcac cgcgcgatcg agctgcagct cgtcgacgcg cagccccgtg
     2041 ctctcggcga gctgcagctg cgccttgtcg agcaccagcc gctccagcac ctggcgctgc
     2101 agcacgccac gctccggcgc cgcgatgcgc tggcgccgca gctcggcggc ggcgcgctcg
     2161 gtgcgctggg cgagttcggt cagcgtgatc acgtccttgt tcacgacggc gaccacccga
     2221 tcgacgagca ctgcgtcgcg cgccgccgcg gcggccggga cgagcgcggc gagcgcgagc
     2281 agcaggccgg cggcgggccg cgtcattcag ttcgccccct cgaagggcgc cgccggctgc
     2341 aggctggggg gcaccagcgt ctggtcgcgc ggattggtca ccgagtagcc cgggacgttg
     2401 cggcggaaca ggttcaccgt ttcgctggag ccgatctggc cgaggccatt gaattcgagc
     2461 tggaacagaa tcgctttcga ggtcacgtcg gttgccgcct gcacgcgctg cgccaccgcc
     2521 cggaacaccc agcagccgcc gttgtactcg atgccaccca gcgcttccag caagcggcgg
     2581 tcgcgcagcg agtaattgta tcgtccgatc gcataccagc cgccctggac cggccactgg
     2641 gccgaaagat cccattgccg cacctgctcc cggttgaagc ggtagctgaa attcagcacc
     2701 ttggcgatct cgggcgcgta gcgcagctgc aggccgtagc gttcggtgcg cgagttgcgc
     2761 gggttgtact gcagcgcgcc gtcgaaggtc cagtggcgcg cgaagcgccc gccggccgag
     2821 gcgagccagt cggactcggt cgaggtacgc agcgtcgagg acgcggtgag gccgacgcgc
     2881 tcctcggcga agtagtagcg ctgcccgagg gtggcgcgca ggcgctcctc gccgcgctcg
     2941 ttcagcaggc gcgaggtgag cgcccaggtg atctggttgg cgtcgccaaa gcggtcgccg
     3001 ccgacgaagc ggttttccga aaacagctgc gcgtagttga agtccgccag cgccgtatcg
     3061 aatagcggca gctgcgattg ctcgcggaac ggcgcgtaga cgtagaacag gcgcggctcg
     3121 agcgtttgcg tcagccgctc gccgaaccag tccgtgccgc ggtcgaaaag cagcccggcg
     3181 tccaggctca gccagggaat gctcaccgtc tggcgctcgg gctggccggc ggcggtgcgg
     3241 ctcagctcgt agcgcgaatg gcgcgcgccg aaccgcggcg tgaggaagta gccgggcgcg
     3301 gtgagcggca tgctgagcac cgggttgacg gtgctgcgct cgccttcgac cagcgtcggg
     3361 tgcgtgaagc gcgcgtactc gatcggcagc gcgccgtcga gcagcccgcc catgtcgttg
     3421 cgcgcgaccg acaggttgag ctgcggcacg cggtggtacg gcggcacgat cggcgcgagc
     3481 gggtcctgca gcgtctggaa gcgctgcacc cgcccctgca ggctgaggcc ggtgccgccg
     3541 aagctgcgcc cgtactgcag atagccgtcg cgctgcaggt tgccggtggc aacctggcgc
     3601 acgtcgctgt acaggtcgac gaaatagcgg tcgtcggaca cgtggttcac gtcgaccttg
     3661 gcggtgagcg ccggggtaaa gcggtgctcg tgctgcaccg aatagccggt gcgcgagcga
     3721 ccgagcagct tgtcctcggg caggtactcc aggcgcgcct cgccgttgaa gctgcggccg
     3781 aggtagcggt acagggtctt cagctgctcg ccgcgcttgg acatgaacgt cggggtgagg
     3841 gtcgcgtcct gctcgggtgc gatgttccag tagaacggca ccgtgagctc acccccgcgc
     3901 agcgtggtct gcgcgtagtg cggcggcagc agcccgctct tgcgccggtt ctccagcggg
     3961 aagctgaagt acggcgtgcc gaggatcggg acatcgaaga agcgcaggcg cgcgccgcgc
     4021 gccacgcccg tttcctcggt gtagtcgagc tcgagctcgc tcgcctcgag ccgccagtcg
     4081 tcgttgcccg gctcgcaggt ggtgaaatgc gcgccgctga aaaggtagcg tccgcgcccc
     4141 ctgaactcga tgcgctcggc gccgccgcgc gcggtctgct tgcgccgcat gaggaaggtg
     4201 ggcgcttcca tgacgccggt gtcgtcggtc gtgtcgtaga tgaggcgcgg cccgaagagg
     4261 cggtctgtgc cctgcgcgac gcgcacgccg ccgtcggcct cgagacggcc gaactcgcgg
     4321 ttgaacttga ggtactcggc gtagatctgg gactcgccct gccggaactc gacgccgccg
     4381 cgcgccgtca cttccaggcc cgagatgccc tcgatcgact gcgcgtcgat ggtcgccggc
     4441 tcgcgggcct cgggcgccgg catctgcgca aacgcggcgc acgccgccag cagcgacgcg
     4501 cagccggcaa cctgggtggc cagcttacgc gctggaaaac gctgcatgaa cgagggagag
     4561 agaccggacc tttgcgtgta aaatcgcaca attccgcgcc aggcgcaacg caactcgtgg
     4621 atcacaggct cgctgcgctc acgcgctggc tcgccgggcc gctcggcggc ccggagttca
     4681 ccctcagcgt cgcttccgcc gacgccagct ttcgccgcta cttccgcgtc acccgcgccg
     4741 atgggcgcag cttgatcgcc atggacgcgc ctcccgagca cgaggactgt gccgcgttcg
     4801 tgcacgtggc gcggctgctg cgcgccgcgg gcgtgaacgc gcccgaagtg cacgccgagg
     4861 atctggcgca ggggttcctc ctgctcaccg atctgggcac cgtcacctac ctcgacgcgc
     4921 tgaacgacgc gaacgccgat gcgctgatgc gcgacgcgat cgccgcgctc ctcaagtggc
     4981 agcaggcgac gcgcgacgcg acgctgccgc cgtacgacga ggcgctgctg cgccgcgagc
     5041 tcgcgctgtt tccggactgg tacgtgggcc ggcatctcaa gcgcgagctg tcggccgcgc
     5101 aacgcgccca atgggaagcg ctgtgcgcgg cgctcgtggc gagcgccctg gcgcagccgc
     5161 cggtgtacgt gcatcgcgac tacatgccga gaaacctcat gctgagcgag ccgaaccccg
     5221 gcgtgctcga tttccaggac gcggtcatcg ggcccatcac ctacgacatc gcctcgctca
     5281 cgcgcgacgc gttcctcagc tgggatgagg cgcgcgtgct cgactggtcg gtgcgctact
     5341 gggaaggcgc gaagcgcgcg ggcctgccgg tggccgccga cttcgcggag cactggcgcg
     5401 cgcaggaatg gatggggctg cagcggcacc tgaaggtgct cggcatcttc gcgcgcctca
     5461 cctaccgcga cggcaaggca agatacgtcg ccgatgcgcc gcgctttctc ggctacgcgc
     5521 gcgcggcggc gcggcgctac gtcgcgctcg cgccgctcgc gcggctgctg gacgagctgc
     5581 acgcatgaag gccatggtcc tcgcggcggg cctgggcacg cgcctgcggc ccctcaccga
     5641 cgcggtgccc aaggcgctgg tcgaggccgg cggcaagccg ctcatcgcct ggcagctcga
     5701 gcgcctggcg cgcgcgggct ttcgcgaggt ggtgatcaac gtgagctggc tcggcgagcg
     5761 catcgaggcg cgcctgggcg acggccgcgc gttcggcctc gccctcgctt attcgcgcga
     5821 ggccgagccg ctcgaatcgg cgggcggcat cgcgcaggcg cgcgaactcc tcggcccggc
     5881 acccttcgcg ctggtgaaca gcgatgtctt ctgcgacttc gacctggcgg cgcttcgccg
     5941 ccgcgcgctc ggcgcgcacc tcgcccacct ggtgctgacg cccaatccgc cgcaccatcc
     6001 cgcgggcgac ttctcgctcg aggcgggcgc ggcgggaaac gccgccgccc cgcgctacac
     6061 ttacacgggt atcgcgctca tcgacccggc gctcgttgcg ccggtgcgct cgggcgagcg
     6121 ggcgcagctc gcgccactgc tgcgtgcggc cgctgcggcg ggacggctct cgggcgagct
     6181 gcacgccggc gcgtggcacg acgtcggcac ggccgagcgc ctggccgagc tcgaggcgcg
     6241 gctcgccgaa accggggaaa ggacatgaag gcgaacgatc tcaaagcgga tgtcgcggtg
     6301 cacgccgagc gccggcgacg ccttgccgca gccctcggcg agggcgtcgc cgtgatcccg
     6361 accgcgccgg agcgcgcgcg caaccgcgac tcgcactacc cctaccgcca cgacagctac
     6421 ttttactacc tcaccggctt ccaggagccc gaggcggtgc tggtgctcgc cggcggcgcc
     6481 gaaccgcgcg ccctgctcta ttgccgcgag aagaaccccg agcgcgagac ctgggacggc
     6541 tttcgccacg ggcccgagaa cgcgcgcgag cgctacggct tcgacgaggc gcaccccgtc
     6601 gccgcgctcg acgaggcgat gccggcactg ctcgccgacc gttccgcgct gtggtatccg
     6661 gtaggcgccg acgccgagtg ggacgcgcgc gccatgcgct ggctgaacgc cgtgcgcgcc
     6721 aaagcccgcg cgggcgtcgc cgcgccgcag acgctgcacg acgtgcgcgc gccgctcgac
     6781 gaaatgcgcc tggtaaagga cgcgcacgag ctcgccgtga tgcgccgcgc ggcggcgatc
     6841 tccgcggcag cgcaccgccg cgccatgctc gccgcgcgcc cggggcgccg cgagtacgaa
     6901 gtcgaagccg agctgctgca cgagttccgg gtgaacggcg cgcagtttcc ggcctatacg
     6961 ccaatcgtcg ccggaggcgc caacgcctgc gtgctgcact acgtgcagaa cgacgcggtg
     7021 ctcgccgacg ggacgctcct gctcatcgac gccggctgcg agctcgaggg ctacgccgcc
     7081 gacatcacgc gcaccttccc ggtcaacggg cgcttcagcg gcgcacagcg cgaggtctac
     7141 gagatcgtgc tcgcggcgca gcgcgcggcg atgggcaagg tgcgcgcggg caatgcctgg
     7201 aacgagccgc acgacgccgc ggtgcgcgtg ctggcgcagg gcatgctcga cctgaagctg
     7261 ctggccggca gcctggacga agcgctggag aaggagacct acaagcgctt ctacatgcac
     7321 cgcacggggc actggctggg catggatgtg cacgacgcgg gcgaatacaa gcgtgcgggc
     7381 gcgtggcgca ggctcgagcc cggcatgacg ctcaccgtgg agccggggct gtacatccgc
     7441 gccgcggacg acgtgcccga gcgcctgcgc gacatcggcg tgaggatcga ggacgacgta
     7501 cacgtcaccg cggccgagtg cgaggtgctg accgccgcgg cgcccaaggc gatggccgag
     7561 atcgaggcgc tgatccgtga cggccggcgc tgacattctc gttcgcgggg cgggcccggt
     7621 cggctgcacc ctggcgctgg cgctgcgcgg atcgcgccac gccgtgcgcc tcgtcgaccc
     7681 gcagcccgcc gtccgcgcgg cctttcgccc catcgcgctg tcgcacgcca gccggctgat
     7741 cctggagcgc gccggtgtct ggggcgactt tcccgtgacg ccgatcatga gcatccgcgt
     7801 ctcgcaggcg ggcgccttcg gccgcacgcg catggacgcg gccgacgccg gcgtgcccgc
     7861 gctcggctac gtcacgcagt acgccgagct gctcgccgcg ctgcgcgcgc gcctcgccat
     7921 cgacgccgac gacgggactg cggcgcgctg cacggtgcat gccgaaggga cggccgcgga
     7981 cctgcgggag cggcgctacg cgcacgacgc ggtggtggcg cgcgtcgcgg ccgagcccgc
     8041 gccccgcgcc gaggccttcg agcgctttac cgccgaggga ccgctcgcgt tgctgccgct
     8101 cgccgggtcc tgggcgctca tctggagcgc gcgtgcgcag cgtgcggccg ccctcgcggc
     8161 tgccgcgccg caggaattcc tcgcgctgct gcaggcggcg tgcggcgagc gtgccgggcg
     8221 cttcaccgcg gtgcacgagc gcgcgcaggt acccctcgcg ctgcgcgtgc gcgcgtcccg
     8281 ggtcaagggc cgtgaggtct acatcggcaa cgcggcgcaa acgctgcatc ccgtggcggg
     8341 ccagggcctg aacctcggcc tgcgcgacgc ctgggatctg gcgcaggcct tgcatgaagc
     8401 cgaggacgcg ggcgacccgg gcgtgctcgc gcgctttgcg gcgctgcgcc gcgtcgacgc
     8461 cggggcgacg atcggcgcca ccgagttcct ggcgcgcgcc tttctcggcg cgcatccgct
     8521 cgcgcgcgcc gcgcgcggcc tcgcgctgac gagtcttgac attctgccgc ccgcgcggcg
     8581 cttttttgcg cggcgcatga tcttcgggcc ttccgcgctg ccctgaactt ttatagaatt
     8641 ttgtttccct tcgcgatcgg aaccctgaac gtcctatgca gctcggcgcg cacgtcctgc
     8701 gcaatgcgtt gttcgctgcc ccgatggcgg gggtcaccga ccggccgttc cgcatcctcg
     8761 cgcgccgcta cggcgcgggc ctagcgacct cggagatggt gtcctcgcgc cccgagctgc
     8821 gcggctcgcg gaagtcacgg ctgcgcgtgg atcacgccgg cgagccgggc ccggtgtcgg
     8881 tgcagatcgc cggcgccgag ccggcgatga tggccgcggc cgcggtctac aacgtcgagc
     8941 gcggcgcaca gatcatcgac atcaacatgg gctgcccggc gaagaaggtc tgcaacgtgt
     9001 acgccggctc ggcactgctc gaggacgagg cgctggtggc gcgcatcctg gaggcggtgg
     9061 tcgcggccgt cgacgtgccg gtgacgctga agatccgcac cggccccgcg cccgtgcggc
     9121 gcaacgccgt gcgcatcgcg aagatcgccg aggactgcgg cgtgcagttg ctcgccgtcc
     9181 acggccgtac gcgcgcctgc ggcttcgccg ggcacgccga gtacgacacc atcggcgagg
     9241 tgaaggcaag cgtgaagctg ccggtgatcg ccaacggcga catcgagacc ccgcaacagg
     9301 cgcacgccgt gctcgagcgc acgggcgccg acggcttgat gatcggccgc gccgccttcg
     9361 gccggccctg gatcttccgc gagatcgagc acttcctcgc caccggcgcg acgctcgcgc
     9421 ccccggggcc cgcggaaatc gaggccgtgg tgcgcgagca cctcgagggc ctgtacggcc
     9481 tctacggcga agagcaggga ctgcgcgtgg cgcgcaagca ccttggctgg tatacgaagg
     9541 cgctgcccga gggcgagcgc ttccgccagg cggtggtgcg cctgcaagcc gcggcgacgc
     9601 agcgcgcggc ggtgaacgac tatttcgcga ggcttgccgc atgagccgcg gcagcgagct
     9661 cggcgatgcc gtcaggcgct ctctcgagcg ctacttcaag gacatggacg gcgagcatcc
     9721 caccgcgatc tacgacatgg tgctcaagca cgtggagaag ccgatgatcg agctggtgct
     9781 cggccgggcc gagggcaacc agtcgcgcgc cgccgcgatg ctcggcatcg accgcaacac
     9841 gctgcgcaag aagatgcagc agctgcgcat caaagggtga gcaagctcaa gcgggcgctg
     9901 atcagcgtct cggacaagtc ggggatcgcg gagttcgcgc gcgcgctcgc gggcctgggc
     9961 gtcgagatcc tgtccaccgg cggcacggcg aagctcctgg agaaggcggg cgtggcggcc
    10021 accgaggtct cggcgcacac cggctttccc gagatgctcg acgggcgcgt gaagacgcgg
    10081 cacccgaaga tccacggcgg catcctggcg cggcgcgaca gcgccgcgca cgtggcggcg
    10141 atcgagcagg cgggcatcgc gcccatcgac ctcgtggtcg tcaacctgta tccgttccag
    10201 gccacggtcg ccgatccgga cagccggctc gaggacgcca tcgagaacat cgacatcggc
    10261 gggcccgcga tgctgcgctc ggcggccaag aaccacgccg gcgtcgccgt ggtggtggat
    10321 cccgccgact acgcgcgggt gctggaggag ctgcgcgcca ccggcgaggt ctcggcgccg
    10381 acgcgcttcg cgctcgccac caaggccttc gcccacaccg ccgcctacga cggcgcgatc
    10441 gccaactacc tctcctcgct cgacgcgaaa ggtgcacgcg gtgcgtttcc cggggtgctg
    10501 tcgctgcagt tcgcgaagct gcaggacatg cgctacggcg agaacccgca ccaggccgcg
    10561 gcgttctacc gcgacacggc gccgcagccc ggcggcatcg ccggttaccg gcagctgcag
    10621 ggcaaggcgc tgtcctacaa caacgtcgcc gacgccgacg ccgcctggga gtgcgtgaag
    10681 agcttcggcg agcccgcttg cgtcatcgtc aagcacgcca acccctgcgg cgtggcgatc
    10741 gccaggagcg cgctcgaggc ctaccgcaag gcgttcaaga ccgacccgac ctcggcgttc
    10801 ggcggcatca tcgccttcaa ccgcgcgctg gacgctgcgg ccgccgcgca gatcggcagg
    10861 cagtttgcgg aagtgatcat cgcgccgcgc gtcgacgccg cggcgcgcgc cgcgttcgca
    10921 ggcaaggcca atctgcgcgt gctggaagtt ccgctcgcgc gtgcgcccaa tgcactggat
    10981 ctcaagcgcg tgggcggcgg gctgctggtg caaagcgccg acgcgcacga gcttgcggcc
    11041 ggcgacctga aggtggtgac caggcgaaag cccggcaagg cacagctcgc cgatctgctg
    11101 ttcgcctggc gcgtggcgaa gttcgtgaag tccaatgcca tcgttttcgt caggggcggc
    11161 atgacggtgg gcgtgggcgc gggccagatg agccgcatcg acagcgcgcg catcgccgcg
    11221 atcaaggcga aggcggccgg gctgacgctg aagggctcgg ttgccgcctc ggacgccttc
    11281 tttccgttcc gcgacggcct cgacgcggtg gccaaggcgg gcgccgtcgc ggtgatccag
    11341 cccggcggca gcgtgcgcga cgacgaggtg atcgccgcgg ccgacgcgcg caaggtggcc
    11401 atggtgttca cgggcgtgcg ccacttccgc cactgagaac aacgatgcat tgcgaagcag
    11461 cacaagcgct gcatcgcaat gcccaacttt cggaaagagc atgaagcttc tggttatcgg
    11521 gtcgggtgga cgggagcacg cgctggcgtg gaagctgtcg cagagcccgc gcgtgcagaa
    11581 agtctatgtg gccccgggca acggcggcac cgcggccgag ccggacgcgg agaacgtgcc
    11641 gctctcggca gtcgacgagc tcgtgcattt cgcgaagaac gaaggcgtct acctcacggt
    11701 ggtcggcccc gaggcgccgc tcgccgcggg cgtggtcgac gcgttccgcg aggctgggct
    11761 gcgcatcttc ggcccgacgc gcgccgctgc gcagctggaa agctccaagg acttcgccaa
    11821 gcgcttcatg gcgcgccacg gcatccccac cgcgcgctac gccacgttcg aggacgccgc
    11881 cgcggccaag gcctacgtcg agaaggaagg cgcgcccatc gtggtcaagg ccgacggtct
    11941 cgcctcgggc aagggggtgg tggtggcgca atcggtcgcc gaggcgcacg cggcgatcga
    12001 ccggctcggc ggcaagctgg tgatcgagca gtgcctgcag ggcgaggaag cgagcttcat
    12061 cgtcatcgcc gacggcgccc acgtgctcgc gctcgccacc agccaggacc acaagcgcct
    12121 gcgcgacggc gacaccggcc cgaacaccgg cggcatgggc gcgtactcgc ctgcgccggt
    12181 ggtcacgccc aaggtgcacg cgcgcgtgat gcgcgagatc atccggcccg ccatcgccgg
    12241 catggcgaag gacggcacgc cctacaccgg gtttctgtac gccggcctga tgatcgatgc
    12301 gtcgggcgcg ccgaagacgc tggaattcaa ctgccgcctc ggcgaccccg aggcgcagcc
    12361 gatcctgctg cggctgaaat cggacctgct cgcgctgctc gacgccgcgc tcgagggccg
    12421 gctcgaaaat gccgaggccg aatgggaccg gcgcgcggcg ctcggcgtgg tgctcgccgc
    12481 gcacggctac cccgaggcgc cgcgacaggg ggaccgcatc gagggcatcc cggcgcccgc
    12541 ggacgactgc cgcgtgttcc aggccggcac gcgcacccag ggcaaggacc tcgttgcgag
    12601 cggcgggcgc gtgctctgcg tcaccgcgct cggcgacaac ctgcgcgcgg cgcgcagccg
    12661 cgcctacgcg gccgtggagc gcatccgctt cgacggcatg cagtaccgca aagacatcgg
    12721 ccaccgcgcg ctgcacaggt gaacctcgcc gccgtcagcg actacttcct cggcctgcag
    12781 ggcgcgatcg tcgcccggct ggaagcgctc gacggcggca agttccgcga agaccgctgg
    12841 tcgcggcccg aaggcggcgg cgggcgcgcc tgcgtcatcg aggacggcgc gctcctcgag
    12901 cgcggcggcg tcaacttctc cagcgtgcag ggtgcgcgcc tgccgccctc ggcgagcgcg
    12961 acgcgcccgc agctcgccgg ccgcgcgttc gaggcgatgg gcgtgtcgct cgtgctgcat
    13021 ccgaggaatc cgtattgccc tacggtgcac atgaacgtgc gctgctttgc cgccggcgac
    13081 gtgtggtggt tcggcggcgg catggacctc acgccgtact acgggttcga ggaggacgcg
    13141 cggcactttc atgcgacctg caaggcggcc gtcggcgcgc gctaccccgg attcaagcgc
    13201 tggtgcgacg agtacttcta cctgaagcat cggcaggagc cccgcggcat cggcggcatc
    13261 ttcttcgacg atctgaacga gccggacttc gacgcctgct tcgcgctggt gcgctcggtg
    13321 ggcgaccatt tcctgacggc ttacgcgccg atcgtggaga aacgcaggca aacgccctac
    13381 ggcgagcgcg agcgcgagtt ccaggcctac cggcgcgggc gctacgtgga gttcaacctg
    13441 gtgtacgacc gggggacgct gttcggcctg cagtcgggcg gcaggaccga ggcgatcctg
    13501 atgtcgctgc cgccgcgcgt ggcgtggcgc tatgactggg tgcccgcggc cggttcgccc
    13561 gaggcaaggc tctacggcga attcctgaag ccgagggact gggtctaggg cagctccgcc
    13621 gcgcggcggt aatgcggctg cgcctccgcg ctgcgctcca gccggtcgag caggcgcgcg
    13681 agctccaggt gcgtggcgcg cgactcctcg aacgacaggc tcgcctccag gtaattctgc
    13741 gccttgcccc agagctcggc gtgcgcgcac aggcgcccca gcgcggcgag cagctgcggg
    13801 tccgactcgt gctcgagcag ccacttctcc gcgcgctcga tgcgccggcg cgcttcttcg
    13861 ccgcgtgcgg gctgctccat cccgggcagc tcgccgtaca gcgcggcgag cgccggcgcc
    13921 cattccttgg caagcgcctt ctccaccgcg tcgcgcgcca ggttcgtcag cccgagcgcg
    13981 agcgcgtggc gcgccgccgc gcccgcgatg cgcgcgatgg attgctcgcg cgaggggatc
    14041 ttgcgccagc gctcctcgaa ggtgccgcga tcggccgctg cctgcgccag cagctcgaca
    14101 tgcgcctgca cacggtactc ctcggcgacg cccggggcga tggcatcgcg cttggcgagc
    14161 tgctgggtga ggcgcagcac ctcttcccag ttgcgcaagc cccgctccgc gcgcagcagc
    14221 agccgcaacg tcgcgaactg ccgcgccccc gagccctgca ggctgtgcag cgcgtcggcc
    14281 gcgctgccgt agtcgcgctc gccgagcgcc atcaccgcgc gcgacagcat ggcggcgccg
    14341 cgctggcccg cttcctgcgc gcgcgtcatc cactgctcgc gccgctcggg cgcgcccagc
    14401 tcgtgcgcgg cgcgcgcggc gatgatcgcc gccaggccgg gcgatgccgc gccgtcgaag
    14461 gcgcgcgtcg cctccttctc ggcgcgcgca tagcggccct cgagccaggc ctgcagcgcc
    14521 gatgcgagcg ccgcctgcgc gcgctcgcgg cgccgccgcg cgcggtacgc gcgcacgtac
    14581 gccggcagcg ccacggtgtg ctgcagcatg cgcacggcga tgaacgcgcc aatgaacgcg
    14641 agcagcgcca gcaccgcgta gagcagcagc gacatctcca cgcgccactc ggcgtacgcg
    14701 aactgcacgt agccgcgctc caggtggccg aacacggcga gcgccgccgc cgcggcgaac
    14761 accgccagca gccagaacag gcccttcatc ccgcggcgcg gtcgcggcgc gccttgaaat
    14821 tgcgcaccgc gtcgaggctg tcggtgatcg acggcggctc gaagctgatc ggcgagccct
    14881 gcagctgctc gagctgcgcg agcgcctcct gcgtggaccg cgcgcgcgca tcgtagtaac
    14941 gcagcagcca gcgccgggct gcgcccacgt cctgctcgaa gcccgcttgg ttgcgcgaga
    15001 gcagcgcaag gcgcgcattg agcagcctca ggcgcaggtt ctcgcgcagg aaataggcct
    15061 gctcgggcgg cagcagcggc ggctgcggcg catccacgcg c
//
LOCUS       DBNHKE010000112        11491 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_654328, whole genome shotgun sequence.
ACCESSION   DBNHKE010000112 DBNHKE010000000
VERSION     DBNHKE010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_654328"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..678
                     /locus_tag="AC16O9_07005"
     CDS             172..678
                     /locus_tag="AC16O9_07005"
                     /inference="COORDINATES: protein motif:HMM:NF051921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7931 domain-containing protein"
                     /protein_id="HIR6516401.1"
                     /translation="MSETDETRPQTEYRPFDGEAEFQQAVERLLAAPGRELRIFDPNL
                     SSLRLNDPERIERLKAFLLESRTRRIYIALHDPDHMTRECPRMMSLLARFAHAIQVNR
                     THEEIRNIQDSFLVLDQAHYVRRPVAQFYRGALGLYDEAEALAMRGRFQEIWAASFPG
                     VSSTTLGL"
     gene            complement(801..1571)
                     /locus_tag="AC16O9_07010"
     CDS             complement(801..1571)
                     /locus_tag="AC16O9_07010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6516402.1"
                     /translation="MRFASLGSGSKGNCLVAEAGASRVLIDCGLSPRETTRRLARLGL
                     EPAALSAILVTHEHDDHVGHAYAFAARHALPVYLTHGTRRAQEGAGADADGVELRLIE
                     GRTPFAVGDVRVEPFTVPHDAREPVQFVLGDGARRLGVLTDLGTSTAHVESMLSGCDA
                     LVLEANHDLGLLWGGEYPKWLKERIGGPFGHLDNAAAGRLLAALERSRLKHVIAAHLS
                     QQNNTEAMARAALAAALGCAAEWVGVATQDEGFGWREL"
     gene            complement(1571..2701)
                     /gene="bamC"
                     /locus_tag="AC16O9_07015"
     CDS             complement(1571..2701)
                     /gene="bamC"
                     /locus_tag="AC16O9_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR6516403.1"
                     /translation="MKRLAAAAAAAAALVLLGACSSTDGLLDGASRIEYKSAQKLPPL
                     DVPPDLTAPARDDRYQVPGAASGTTLSGYQAGRTQPAPPGASSLLASVAGVRVERAGD
                     QRWLVVNEPAEKLWPLVKTFWQENGLLIAREVPEAGVLETEWAENRAKIPDSWIRRTL
                     GSLLESLHSSGERDKYRTRLEPAPGGGTEIYISHRGLAEVYVSEDRTQTAWQPRPPDP
                     GLEAEFLRRLMVRLGTPEGEARARVANAQPAAQRATLKKGLDGAELLEVLEPFDRAWR
                     RVGLALDRVGFTVEDRDRQKGVYFVRYADPQAELKKKDDGLLGRLAFWRDDKPKVKAE
                     QYRVAVTAADDASRVRVLDASGAAENSPTASRILVLLHEQLK"
     gene            complement(2698..3606)
                     /gene="dapA"
                     /locus_tag="AC16O9_07020"
     CDS             complement(2698..3606)
                     /gene="dapA"
                     /locus_tag="AC16O9_07020"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR6516404.1"
                     /translation="MITGSLVAIVTPMHEDGRLDMARFRELIDWHVAEGTDGIVVVGT
                     TGESPTVDFDEHKELIRVAVDHAKGRIPIVAGTGGNSTAEAVELTESAKKAGATACLS
                     VVPYYNKPTQEGLYRHFRKVAETVGLPMILYNVPGRTVADLQNDTVLRLAQVPGIIGI
                     KDATANIERGTDLVKRTPRGFAVYSGEDSTALALILLGGQGVISVTANVAPRLMHEMC
                     AAALVGDVKKAREINLRLLPLHQRLFVEANPIPVKWALAQMGRIEAGLRLPMTPLSDR
                     FHDALREALAEAGINIAGLRVVEKRA"
     gene            complement(3643..4152)
                     /locus_tag="AC16O9_07025"
     CDS             complement(3643..4152)
                     /locus_tag="AC16O9_07025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516405.1"
                     /translation="MKEHDIVAPSAWVVRWAPCIARGRVLDLASGSGRHARLLAGLGH
                     EVTAVDREPQSIAGVRFVRANLEDGSPWPLPGAQFQGIVVANYLHRPLFSLIEGALAP
                     GGVLIYETFMRGNERLGRPSNPAFLLAPGELWTAFAGLHIIAFEQGLSLHPKPAMVQR
                     ICARRAGNG"
     gene            4170..5336
                     /locus_tag="AC16O9_07030"
     CDS             4170..5336
                     /locus_tag="AC16O9_07030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HIR6516406.1"
                     /translation="MPTQTIRFPGRQGQELAARLDTPAEAPRAFALFAHCFTCSKDSK
                     AAAYVSQALAARGIATLRFDFSSLEFSSNLDDLVAAAEWLRSTHQAPQVLAGHSLGGA
                     AVLAVAGRIPAVRAVATIGAPFDPAHVAHLVKDPKETGDDLQVSIAGRQFRVAKNFFD
                     DLAGQKAGAAIGALRKPLLVFHSPLDAVVDIENASKIFLAAKHPKSFVSLDRADHLLT
                     QKEDAQYAATVLAAWASRYLEPEAQVAPAVSGVRVAEAGEGRFAQLVTMGRHRLRADE
                     PASVGGEDSGPSPYDLLLAGLGACTSMTVRMYAAQKNWPLERVAVDLKHDKVHAADCA
                     ECETREGKIDKIERVLTLEGDLDEAQRARLLEIANKCPVHRTLHSEVWVPTTLK"
     gene            complement(5354..5737)
                     /locus_tag="AC16O9_07035"
     CDS             complement(5354..5737)
                     /locus_tag="AC16O9_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516407.1"
                     /translation="MTIKLITWIAGMAMLSGGSPALAQDIRVSVSGEVAPGVYGRVDI
                     SNGPRPPLVSPDPIVVVRQARAPGPVYLHVPPGHAKNWAKHCRKYDACSRPVYFVKSA
                     EYDPGYKHPGKGAQKSAGKGKGRDK"
     gene            complement(5762..6157)
                     /locus_tag="AC16O9_07040"
     CDS             complement(5762..6157)
                     /locus_tag="AC16O9_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516408.1"
                     /translation="MNRTRNFIAILGIAGLAACVHPGNVDVGVRASGEVAPGVYGRVD
                     VRNRPPPLVYPQPVIIVEQPRRAQPVYLHVPPGHAKNWRKHCHKYDACGTPVYFVKSA
                     EYEPGYQRKGRGRGRDDDDDDDDRRDRRR"
     gene            complement(6257..6388)
                     /locus_tag="AC16O9_07045"
     CDS             complement(6257..6388)
                     /locus_tag="AC16O9_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516409.1"
                     /translation="MNVWKWLGLAALAGASALIFAAYQRPDTMVSLTSAFLALCGFK"
     gene            complement(6385..7596)
                     /locus_tag="AC16O9_07050"
     CDS             complement(6385..7596)
                     /locus_tag="AC16O9_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6516410.1"
                     /translation="MSSSALRSPTAVLVACCIILCIGFGVRSGFGLFLQPMSLDNGWG
                     RETFSFAMAIQNILWGSFGPFAGGVADRYGAGRVVVLCGIAYVLGLVVMAFTETALAL
                     HVGSGFLIGLALSGSTFAVLLAVIGRVTPPEKRSTALGIAVAAGSFGQFAILPITQLM
                     ISGFGWRNALLGLAMIAALMLPLAFFARGKAPSHAGPQQSLGGALHEALRERGFHLLF
                     WGYFVCGFHITMLTVHLPSYVTDNGLKPEHGMTALALIGLCNMIGSFTAGWLGGRVSK
                     KYLLSAIYTLRAIAITVLISFPLSPLTLYGFAVVIGFLWLSTVPLTNGLVGQIFGMRH
                     MGMLAGVVFFGHQIGSFIGVWLAGWMYDQSGSYNSALVLSIGLGVFAALVNLPVNEKP
                     LAERRPAAAAA"
     gene            complement(7779..8699)
                     /locus_tag="AC16O9_07055"
     CDS             complement(7779..8699)
                     /locus_tag="AC16O9_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HIR6516411.1"
                     /translation="MTLTELRYIVAVARERHFGHAAQSCFVSQPTLSVAIKKLEEELG
                     VVLFERGPGEVTVTPIGQRVVEQAQRVLEEAARVKDIAQSAKDPLAGPLRLGAIYTIG
                     PYLLPKLIPVLRKKAPTMQLLIQENYTHKLGEALKQGEVDVIVVALPFAEPGVTTRAV
                     YDEPFMVALPQGHPLEKKKHISAEELTRESLLLLGAGHCFRDQVLDLCSTVQRGPRGA
                     LARTLEGGSLETIRQMVASGVGLTVLPTTAVNSGEASDGLIRFRPFMRPVPDRRVVLA
                     WRKSFPRPQAIEALREAILACELAQVVKIQ"
     gene            complement(8696..10045)
                     /locus_tag="AC16O9_07060"
     CDS             complement(8696..10045)
                     /locus_tag="AC16O9_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6516412.1"
                     /translation="MASDKSAVTSSGPRPLLSVGDGIFLTVGMVVGVGIFKLPSLVAG
                     NTSSATVFLLLWVVGGIVSLCGALVYAELAARHAETGGEYVFLQRGLGGGAAFLFAWS
                     RMTVIQTGAIAAVAFAFGEYASQILALGRHSTALWAALAVAGLTALNVAGTWQSKTTQ
                     KVTQIVLIAVLVLLALVAFTASPPAGEIARSAPSGNVFGVAMIFVLFTFGGWNEAAYL
                     SGEVREARRNMVRVLVWGILAVTALYLLVNAAYLSALGLEGMRASSAVAADAMRAALG
                     DGAAVAVAVVVCVSALTTMNAAIFTGARTNWALGRDYRLFGGLGAWRESGSTPANSLL
                     LQGVIALALIGAGSATPDGFGAMVAYTAPVFWTFFLLTGATLFVFRARGGEAPAFRVP
                     LYPVVPIAFLAMCAFMLWSAIDYIRNPVYGPKFGVMVLAGLAVMALGVPLYFLARRK"
     gene            complement(10021..10848)
                     /locus_tag="AC16O9_07065"
     CDS             complement(10021..10848)
                     /locus_tag="AC16O9_07065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6516413.1"
                     /translation="MLISAAPAAAQSWAWDDGSVPFVVTPAEIIERMLRMAEVGKGDH
                     LIDLGSGDGRIVIAAAQRGATALGVDLDPDLVRIASANARRAGVAERTQFQVRDLFET
                     DLSQASVVAFYLLPEFNAKLLPRLLALKPGTRIVSHDGGIGEWPPDERLEMRVPEKAV
                     GVGGLSRVELWVVPGDARGRWVSEVPQHGGRWSFDIAQRFQELEVEVRAQGRDLLVRA
                     SRLRGAEIKLAVTGVVGARAWNHLFAGRVDGGRIEGELTISDGEQTRTHPWRATRAQ"
     gene            10893..10968
                     /locus_tag="AC16O9_07070"
     tRNA            10893..10968
                     /locus_tag="AC16O9_07070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10926..10928,aa:Arg,seq:cct)
BASE COUNT         1816 a         4130 c         3872 g         1673 t
ORIGIN      
        1 cacgcaggta ctgacgctgg gtgcgcgcgg cttcgtcaac ggcgagcccg tgccggcgag
       61 cgccgcgctg cgcgcgctcg ccgaccggcg ccggggacgg ctcccggccg cgcttgccga
      121 ttccgcctac cgctggtatt tgtcgggcta cctgcacttg gagcgcgcgc gatgagcgag
      181 acggatgaga cgcggccgca gaccgaatac cggccgttcg acggcgaggc cgagttccag
      241 caggcggtgg aacggctgct cgccgcgccg ggccgcgagc tgcgcatctt cgatcccaac
      301 ctgtcgtcgc tgcgactgaa cgaccccgag cgcatcgagc gcctgaaggc gttcctgctg
      361 gagagccgca cgcggcgcat ctacatcgcg ctgcacgacc ccgaccacat gacgcgcgag
      421 tgcccgcgca tgatgtcgct gctcgcgcgt ttcgcgcacg cgatccaggt gaaccgcacg
      481 cacgaggaga tccgcaacat ccaggacagc ttcctggtcc tcgaccaggc gcactacgtg
      541 cgccggccgg tggcgcagtt ctaccgcggc gccctcggcc tgtacgacga ggcggaggcg
      601 ctcgcgatgc gcgggcgatt ccaggagatc tgggcggcgt cgttcccggg cgtctcgagc
      661 acgacgctcg ggctgtaaag gggttcggcc tttcaatgca gctcggcttt tccggccggg
      721 agattcatcc ggccggaaaa gccgagacat agggaaacca tcgcaggcac cggcgccttc
      781 ggctcgccgg ctccgactct ttacagctcc cgccagccga agccctcgtc ctgggtggcg
      841 acgcccaccc attcggctgc gcagccgagc gccgccgcca gcgcggcgcg cgccatcgcc
      901 tcggtattgt tctgctgcga caggtgcgcg gcgatcacgt gcttgagccg cgagcgctcg
      961 agcgcagcga gcagccgccc ggcggccgcg ttgtcgagat ggccgaaggg cccgccgatg
     1021 cgctccttca gccacttcgg gtactcgccg ccccacagca ggccgaggtc gtggttcgcc
     1081 tccagcacca gcgcgtcgca gcccgagagc atcgactcga cgtgcgcggt cgaggtgccg
     1141 aggtcggtga gcacgccgag ccgccgcgcg ccgtcgccga gcacgaactg cacgggctcg
     1201 cgggcgtcgt gcggcaccgt gaacggctcc acgcgcacgt cgccgacggc gaacggcgtg
     1261 cgcccctcga tcaggcgcag ctcgacgcca tcggcgtcgg cgcccgcgcc ctcctgggcg
     1321 cgccgcgtgc cgtgcgtcag gtacaccggc agcgcgtggc gcgcggcgaa cgcgtaggca
     1381 tggccgacat ggtcgtcgtg ctcgtgcgtg acgaggatcg cgctcagcgc cgcgggctcg
     1441 agcccgaggc gcgcgaggcg ccgcgtcgtc tcgcgcggcg acaggccgca gtcgatcagc
     1501 acccggctgg cgcccgcctc ggccaccagg cagttgccct tgctgccgct gccgagcgac
     1561 gcgaaacgca ctacttcagt tgctcgtgca gcaggacaag gatgcggctc gcggtgggtg
     1621 aattctccgc cgcgccgctc gcatcgagca cgcgcacgcg gctcgcgtcg tcggcggccg
     1681 tcacggcgac gcggtactgc tcggctttca ccttgggctt gtcgtcacgc cagaacgcaa
     1741 ggcgcccgag cagcccgtcg tccttcttct tcagctcggc ctgcgggtcg gcatagcgca
     1801 cgaagtacac gcccttctgc cggtcgcggt cctcgaccgt gaagccgacg cgatcgagcg
     1861 cgaggccgac gcgccgccat gcgcgatcga acggctccag cacctcgagc agctcggcgc
     1921 cgtcgagccc cttcttcagc gtcgcgcgct gcgccgccgg ctgcgcgttg gcgacgcgcg
     1981 cgcgcgcctc gccctcgggc gtgcccaggc gcaccatcag ccggcgcagg aattcggctt
     2041 ccagccccgg atccggcggg cgcggctgcc acgcggtctg cgtgcggtcc tcgctcacgt
     2101 acacctcggc gaggccgcgg tggctgatgt agatctcggt gccgccgccc ggcgccggct
     2161 ccaggcgcgt gcggtacttg tcgcgttccc ccgaggaatg cagcgactcg agcagcgacc
     2221 cgagcgtgcg ccgtatccag ctgtcgggaa tcttggcgcg gttctcggcc cactcggtct
     2281 cgagcacccc ggcctcggga acctcgcgcg cgatgagcag cccgttctcc tgccagaagg
     2341 tcttcaccag cggccagagc ttctccgcgg gctcgttgac caccagccag cgctggtctc
     2401 cggcgcgctc gacgcgcacg cccgcgaccg aggccagcag cgagctcgcg cccgggggcg
     2461 ccggctgcgt ccggccggcc tggtagcccg agagcgtggt gccgctcgcg gcgccgggca
     2521 cctggtagcg gtcatcgcgc gcgggcgccg tcaggtccgg cggcacatcg agcggcggca
     2581 gtttctgcgc cgacttgtac tcgatgcggc tggcgccgtc gagcaggccg tcggtgctgc
     2641 tgcacgcgcc gagcagcacg agcgcggccg ccgcggccgc cgcggccgca agccgcttca
     2701 tgcgcgcttt tcgaccacgc gcaggccggc gatgttgatg cccgcctcgg caagcgcctc
     2761 gcgcaacgcg tcgtgaaagc gatccgagag cggcgtcatc ggcagcctga ggcccgcttc
     2821 gatgcggccc atctgcgcca gcgcccactt caccgggatc ggattcgctt cgacgaacaa
     2881 gcgctggtgc agcggcaaca ggcgcaggtt gatctcgcgc gccttcttca cgtcgccaac
     2941 cagcgccgcg gcgcacatct cgtgcatcag gcgcggcgcg acgttggcgg tgaccgagat
     3001 cacgccctgc ccgcccagca ggatcagcgc cagcgcggtc gagtcctcgc cgctgtacac
     3061 ggcaaagccg cgcggcgtgc gcttcaccag gtcggtgccg cgctcgatgt tggcggtagc
     3121 gtccttgatg ccgatgatgc cgggcacctg cgcgaggcgc agcaccgtgt cgttttggag
     3181 gtcggcgacc gtgcgccccg gcacgttgta caggatcatc ggcagcccga ccgtctccgc
     3241 caccttgcgg aagtgccggt acaggccttc ctgcgtcggc ttgttgtagt acggcaccac
     3301 ggacagacac gcggtggcgc cggccttctt cgccgactcg gtaagctcga cggcttccgc
     3361 cgtggaattg ccgccggtgc cggcaacgat cggaatgcgg cccttggcgt ggtcgacggc
     3421 cacgcggatc agctccttgt gctcgtcgaa gtccacggtg ggcgattcgc cggtggtgcc
     3481 gacaacgacg atgccgtcgg tgccctcggc gacgtgccag tcgatcagct cgcgaaaacg
     3541 ggccatgtcg aggcggccgt cctcgtgcat cggggtgacg atcgcgacca ggctgccggt
     3601 aatcataagt gcacctcctt caacttcgca gtgcctgcga ttttatccgt tccctgcccg
     3661 ccgggcgcat atccgctgca ccatggcggg cttgggatgc aggctgagtc cctgctcgaa
     3721 ggcgatgatg tgcagaccgg cgaacgccgt ccacagttcc ccgggggcga gcaggaacgc
     3781 ggggttggac ggcctgccca agcgctcgtt gccgcgcatg aacgtctcgt agatcagcac
     3841 gccgcccggg gcgagcgcgc cttcgatcag cgaaaaaagc ggccggtgca ggtaattggc
     3901 gacgacgatg ccctggaact gcgccccggg cagcggccag ggactgccgt cttccaggtt
     3961 ggcgcgcaca aagcgcacgc cggcgatcga ctgcggctcg cggtcgaccg ccgttacctc
     4021 gtggccgagt ccggcgagca ggcgcgcatg ccggccgctg ccgctcgcga ggtcgagcac
     4081 ccgaccccgc gcaatgcacg gcgcccagcg cacgacccac gcggagggcg cgacaatgtc
     4141 gtgctccttc accccgccga gtctaaatca tgcccaccca gaccattcgc tttcccggcc
     4201 gccagggcca agagcttgcc gcgcggctcg ataccccggc ggaggcgccg cgcgcctttg
     4261 ccctgtttgc gcactgcttc acctgctcca aggacagcaa ggccgcggcc tacgtgtcgc
     4321 aggcgctcgc cgcgcgcggc attgccacgc tgcgcttcga tttctccagc ctcgagtttt
     4381 cctcgaatct cgatgacctc gtcgccgccg ccgaatggct gcgctctacg catcaggcgc
     4441 cgcaggtcct cgccggccac agcctcggcg gcgccgccgt gctcgcggtg gcgggacgga
     4501 tccccgcggt gcgcgcggtc gcgacgattg gcgcgccgtt cgatcccgcg catgtcgcgc
     4561 acctcgtcaa ggatccgaag gaaacgggcg acgatctgca ggtgagcatc gccggccggc
     4621 aattccgcgt ggcaaagaac ttcttcgacg atctcgccgg gcagaaagcg ggcgccgcga
     4681 tcggcgcgct gcgcaagccg ctgctcgtgt tccactctcc gcttgacgcc gtggtcgaca
     4741 tcgagaacgc ctcgaagatc tttctcgctg ccaagcatcc gaagagcttt gtctcgctcg
     4801 atcgcgccga ccacctgctc acgcaaaagg aagacgcgca gtacgccgcc acggtgctcg
     4861 ccgcctgggc gtcacgctat ctcgagcccg aggcgcaggt cgcaccggcc gtgtcgggcg
     4921 tgcgcgtcgc cgaagcaggt gaaggccgct tcgcgcagct cgtcacgatg gggcgccacc
     4981 gcctgcgtgc cgacgagccg gcgtccgtgg gcggcgagga cagcggcccg agcccctacg
     5041 acctgctgct cgcaggcctg ggcgcgtgca cctcgatgac ggtgcgcatg tacgcggcgc
     5101 agaagaactg gccgctcgag cgcgtcgcgg tggacctgaa gcacgacaag gtccacgccg
     5161 ccgattgcgc ggagtgcgag acgcgcgagg gaaagatcga caagatcgag cgcgtcctga
     5221 ccctggaagg cgacctcgac gaggcgcagc gcgcgcggct cttggaaatc gccaacaagt
     5281 gcccggtgca ccgcaccctg cactcggaag tgtgggtgcc gacgacgctt aaatagggac
     5341 aggcctcatt tagctatttg tcgcgccctt tgcctttccc ggccgacttc tgcgcgcctt
     5401 tgcccgggtg cttgtagcca gggtcgtact ccgccgactt cacgaagtac accggccggc
     5461 tgcaggcatc gtatttcctg cagtgcttgg cccagttctt cgcatgcccg ggcggcacgt
     5521 gcaggtagac cgggccgggt gcgcgcgcct gccgcaccac tactatggga tccgggctga
     5581 cgagcggcgg gcgggggccg ttggagatgt cgacgcggcc gtagacgccg ggcgccactt
     5641 cgccggagac gctgacacgg atgtcctgcg ccagcgcggg cgacccgccg ctcaacatcg
     5701 ccatgccggc gatccacgtg atcaatttga ttgtcatctc tcggcctccg aatgaaggat
     5761 cctagcgccg ccgatcgcga cgatcgtcgt cgtcatcgtc gtcatcgcgg cccctgcccc
     5821 tgccctttct ctggtagccg ggctcgtatt ccgcggactt cacgaagtac accggcgtgc
     5881 cgcaggcgtc gtacttgtgg cagtgcttgc gccagttctt cgcgtgcccc ggcggcacgt
     5941 gcaggtacac gggctgcgcg cgccgcggct gctcgacgat gatcaccggc tgcgggtaca
     6001 cgaggggcgg cggccggttg cgcacgtcga cgcgaccgta gacgcccggc gcaacctcgc
     6061 ccgatgccct cacgccgacg tcgacgttgc ccggatgcac gcaagccgca aggccggcaa
     6121 tgccaagaat tgcgatgaag ttccgagtac ggttcacagt gggtccccgt cgttggttgc
     6181 acgcatcatg agccagcggg ctttcgagcg caaggcagtt cgtcgccgcg ccacgacagg
     6241 gaattgaggc cagtccctac ttgaagccgc agagcgcgag gaaggcgctg gtgagcgaga
     6301 ccatcgtgtc gggccgctgg tacgcggcga aaatcaatgc gctcgcgcca gcgagcgccg
     6361 cgagcccgag ccatttccaa acgttcacgc ggcggcggcc gcgggacgcc tctccgccag
     6421 cggcttctcg ttgaccggca ggttgacgag cgcggcgaac acgcccagac cgatggacag
     6481 gaccagggcg ctgttgtagc tgccgctctg gtcgtacatc cagccggcga gccacacgcc
     6541 gatgaagctg ccgatctggt ggccgaagaa caccacgccc gccagcatgc ccatgtggcg
     6601 catgccgaag atctggccga ccaggccatt ggtgagcggc accgtcgaga gccacagaaa
     6661 gccgatcacg accgcaaagc cgtatagcgt cagcgggctg agcggaaacg agatcagcac
     6721 cgtgatggcg atggcgcgca gcgtgtagat ggccgagagc aggtacttct tgctgacgcg
     6781 cccgccaagc catccggcgg tgaacgagcc gatcatgttg cacagcccga tcagcgcaag
     6841 cgccgtcatg ccgtgctcgg gcttgaggcc gttgtcggtg acgtaggacg gcagatgcac
     6901 ggtgagcatg gtgatgtgga agccgcacac gaaatagccc cagaacagca ggtgaaagcc
     6961 gcgctcgcgc agcgcctcgt gcagcgcccc gcccagcgac tgctgcggcc ccgcgtgcga
     7021 cggcgccttg ccgcgcgcaa aaaaggcgag cggcaacatg agcgcggcga tcatcgcgag
     7081 cccgagcagc gcattgcgcc agccgaagcc cgagatcatc agctgcgtga tcggcaggat
     7141 ggcgaactgc ccgaaggagc ccgcggccac cgcgatgccg agcgcggtgc tgcgcttctc
     7201 cggcggcgtg acgcggccga tgacggcgag cagcacggcg aaggtgctgc ccgagagcgc
     7261 gagaccgatg agaaagccag agccgacatg cagcgccagc gccgtttcgg tgaacgccat
     7321 cacgaccagg ccgagcacgt aggcgatccc gcagagcacc accacccgcc ccgcgccgta
     7381 gcgatcggca acgccgccgg cgaacggtcc gaaacttccc cacaggatgt tctggatcgc
     7441 catggcaaag gagaacgtct cgcgccccca gccgttgtcc aggctcatcg gctgcaggaa
     7501 caggccgaac cccgagcgca cgccgaaacc gatgcaaagg atgatgcagc acgcgacgag
     7561 gacggcggtg gggctgcgca acgcggagga gctcatgggc tcagtttagc tcagggggtg
     7621 caacagacct ctacagttcc acgcaaatcg acacggcagg taccggctct tcgctttggc
     7681 ttttccatac gtctcggctt ttctggcggg aagattcacc ccgccagaaa agccgagata
     7741 tataaaggtc aaaaccgcgc aggcctctgc gtcgccgcct actggatctt gaccacctgc
     7801 gccagctcgc acgccagaat cgcctcgcgc agcgcctcga tcgcctgcgg ccgcgggaag
     7861 ctcttgcgcc acgccagcac cacgcgccgg tcgggcaccg gccgcatgaa cggccggaag
     7921 cggatcaggc cgtcgcttgc ttcgcccgaa ttcaccgcgg tcgtgggcag cacggtgagc
     7981 ccgacgccgc tcgccaccat ctggcggatg gtctcgagcg agccgccctc gagcgtgcgc
     8041 gcgagcgcgc cgcgcgggcc gcgctgtacc gtcgagcaca ggtcgagcac ctggtcgcgg
     8101 aagcagtgcc ccgcgcccag cagcagcagg ctctcgcgcg tcagctcctc ggcggagatg
     8161 tgctttttct tctccagcgg atggccctgc ggcagcgcca ccatgaaggg ctcgtcgtac
     8221 accgcgcgcg tggtcacgcc gggctcggcg aacggcagcg ccacgacgat gacgtcgacc
     8281 tcgccctgct tcagcgcttc gccgagcttg tgcgtgtagt tctcctggat caggagctgc
     8341 atcgtcggcg ccttcttgcg cagcaccggg atgagcttgg gcagcaggta cggcccgatg
     8401 gtgtagatcg cgcccaggcg caagggcccg gcgagcgggt ccttcgcgct ttgcgcgatg
     8461 tccttcacgc gcgcggcttc ctcgagcacg cgctgcgcct gctccaccac gcgctggccg
     8521 atcggcgtca ccgtcacctc gccggggccg cgctcgaaca gcaccacgcc gagctcctcc
     8581 tccagcttct tgatcgccac ggacaacgta ggctgcgaca cgaagcagga ctgcgcggcg
     8641 tggccgaagt ggcgctcgcg cgcgacggcg acgatgtagc gcagctcggt gagcgtcact
     8701 tgcggcgcgc gaggaagtac agcgggacgc cgagcgccat cacggcgagg cccgccagca
     8761 ccatgacgcc gaacttcggt ccgtagaccg gattgcggat gtagtcgatc gccgaccaca
     8821 gcatgaacgc gcacatggcg agaaaggcga tcggcacgac cgggtacagc ggcacgcgga
     8881 acgccggcgc ctcgcccccg cgcgcgcgga acacgaacag cgtcgccccg gtcagcagga
     8941 agaacgtcca gaagaccggc gcagtgtagg cgaccatcgc gccaaagccg tcgggcgtcg
     9001 ccgatcccgc gccgatcagg gcaagcgcga tgacgccctg cagcagcaga gagttggcgg
     9061 gcgtgctgcc actttcgcgc caggcgccca gcccgccgaa caggcggtag tcccggccca
     9121 gcgcccagtt ggtgcgcgca ccggtgaaga tcgccgcgtt catcgtggtc agcgccgaaa
     9181 cgcagaccac cacggccacc gccaccgcgg cgccgtcgcc cagggccgcg cgcatggcgt
     9241 cggccgccac cgcgctcgag gcgcgcattc cctccagccc gagcgccgag aggtaggccg
     9301 cgttgaccag caggtaaagc gcggtcactg cgagaatgcc ccatacgagc acgcgcacca
     9361 tgttgcgccg cgcttcgcgc acctcgccgg agaggtaggc cgcttcgttc cagccgccga
     9421 aggtgaacag cacgaagatc atcgccaccc cgaacacgtt gccgctgggc gccgagcgcg
     9481 cgatctcgcc ggccggcgga ctggcggtga acgccaccag cgcgagcagc accagcaccg
     9541 cgatcagcac gatctgcgtc accttctgcg tggtcttcga ctgccaggtg cccgccacgt
     9601 tgagcgccgt cagcccggcc accgcgagcg ctgcccacag cgcggtgctg tgcctgccga
     9661 gcgccaggat ctggctggcg tactcgccga aggcgaacgc cacagcggcg atggcgccgg
     9721 tctggatcac ggtcatgcgc gaccaggcga acaggaacgc cgcgccgccg cccaggccgc
     9781 gctgcaggaa gacatactcg cccccggttt ccgcatgccg cgccgccagc tccgcgtaca
     9841 ccagcgcgcc gcacagcgag acgatgcccc ccaccaccca cagcagcagg aataccgtcg
     9901 cgctggaggt attgcctgcc acgagcgacg gcagcttgaa gatgccgacg ccgaccacca
     9961 tgccgacggt gaggaagatg ccgtcaccca ccgacagcag gggccgcggc ccgctcgaag
    10021 tcactgcgct cttgtcgctc gccatggatg cgtcctcgtc tgctcgccgt ctgaaatggt
    10081 cagttcgccc tcgatgcgcc cgccgtcgac gcgtcccgcg aacagatggt tccacgcccg
    10141 cgcgccgacg acgccggtga ccgcgagctt gatctccgca ccgcgcaggc ggctcgcgcg
    10201 caccagcagg tcgcggccct gcgcgcgcac ctccacctcg agttcctgga agcgttgcgc
    10261 gatgtcgaag ctccagcgcc cgccgtgctg cggcacctcg ctcacccagc gcccgcgcgc
    10321 atcccccggc accacccaga gctcgacgcg cgacagcccg ccgacgccga ccgccttctc
    10381 gggcacgcgc atctcgaggc gctcgtcggg cggccactcg ccgatgccgc cgtcgtgcga
    10441 cacgatgcgc gtgccgggct tgagcgcgag cagccgcggc aggagcttgg cgttgaactc
    10501 gggcagcagg tagaacgcga ccacgctcgc ctgcgacagg tcggtttcga acaggtcgcg
    10561 cacctggaac tgcgtgcgct ccgcgacccc ggcgcgtctg gcattcgcgc tcgcgatgcg
    10621 cacgaggtcg ggatcgaggt ccacgccgag cgcggtcgcg ccgcgctgtg ccgccgcgat
    10681 cacgatacgc ccgtcgcccg agccgaggtc gatgaggtgg tcgcccttgc cgacctccgc
    10741 catgcgcagc atgcgctcga tgatctcggc cggcgtgacc acgaacggca cgctgccgtc
    10801 gtcccaggcc cagctttgcg cggcggcggg cgcggcggag atcagcaacg cgacggagag
    10861 gagaaatcgc aagcggtaga atcggggctc gcgccccggt agctcagtgg atagagcagc
    10921 cccctcctaa ggggcaggtc gcacgttcga ttcgtgtctg gggcgccatg aatccacact
    10981 ttacgacgga actcccggct gcggcagaac gcctggcgcc gacgcctccg caagcgccgc
    11041 gttaatgcga gcctggcgga gagggcgatc gcagggcttc atgccggcgc aaagtgtacc
    11101 cgagcgcagc gctccatcgt gcgcttgaag ccatccgccg acactggatt gcgcgccagg
    11161 ctgcgaaggc cgcctgggcc aagctgaacc tcaggatcca cgaagtcgac ccgctttttt
    11221 gcccacggtg cggcgaggct atgcgcgcga tcgcgccaat gccggctatc gccagatcgc
    11281 cgcggccttc aaggcggtgc accagtagcc ttccgcgcac gcggagagga aggcccagcg
    11341 ggccgcgcgt gttatgcgcg agctgcgggt cgagcaggaa gcgaaattgg cctgagctgc
    11401 gctccggcgg cgcctatact ttcgccgtca tgccccgcct cgccctggcg ctcgccctct
    11461 tcctcgcccc gctcgccgcg gccgctcagg a
//
LOCUS       DBNHKE010000113        12244 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1289129, whole genome shotgun sequence.
ACCESSION   DBNHKE010000113 DBNHKE010000000
VERSION     DBNHKE010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1289129"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="AC16O9_07075"
     CDS             complement(<1..677)
                     /locus_tag="AC16O9_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR6516414.1"
                     /translation="MRTSVLALLAAAALALAGCAAPVEPDETASWSVERLHGEARDAM
                     RERDWAKAIKYLEKLEARFPYGPYAQQAQLDVAWAHWKFDEPAAAIAAVERFIKLYPN
                     HAALDYAYYLKGLVNFTENQGLFAILSDPDLAERDPKAAREAFAAFREVVTRFPGSKY
                     AEDSAARMRYLVNSLARHEVHVARYYMKRGAFVAAANRAQAAVADYPQAPAVEEALFI
                     MLQAYRAL"
     gene            747..1646
                     /locus_tag="AC16O9_07080"
     CDS             747..1646
                     /locus_tag="AC16O9_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6516415.1"
                     /translation="MSAEAPRALAGLRLDQALARMFPQYSRSRLQAWLAEGHITVDGA
                     RATARALVRGGERVALSPPAVPQGAPRAQRMPLKVAYEDADLVVIDKPAGLVVHPGAG
                     QPDRTLMNALLAHAPQLAGVPRAGIVHRLDKDTTGLIVVAKTLEAQVSLVRQLGERSM
                     RRVYWAVVQGDPPASGVIDAPVGRDVRARTRMAVTRRGKDAVTRYRVLERFGRAALVE
                     CRLETGRTHQIRVHFQHIRHPIIGEATYRRGTRHGIGFTRQALHAVELSLKHPRSGQP
                     MTWRAEPPADFRGLLKRLRNDSP"
     gene            1633..2274
                     /gene="pgeF"
                     /locus_tag="AC16O9_07085"
     CDS             1633..2274
                     /gene="pgeF"
                     /locus_tag="AC16O9_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR6516416.1"
                     /translation="MIRPDWPAPPGVRALVTTRGEPDPPLPAPPAWLQQVHGTRVVDA
                     AAARGKPQADASVARVPHAVCAVQAADCLPVLLADDAGAVVGAAHAGWRGLAAGVIEA
                     TVAEMQVPGARLIAWLGPAIGPAHYEVGADVRNAFLARDAAAAQAFVPNRPGHWLLDL
                     YAVARQRLAALGVARVYGGDFCTYSDPTRFPSWRRERTRERIAAFVWLESRSP"
     gene            2271..3011
                     /locus_tag="AC16O9_07090"
     CDS             2271..3011
                     /locus_tag="AC16O9_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR6516417.1"
                     /translation="MTLTWIVLACLAGGVLSVGAAAFALVLRAKWIPMLVSFAIGTLL
                     GAAFLEVLPHAFAEGDARSVATAILGGILGFFLLEKLLLWRHSHEHGDADGPAHHQEH
                     ARAGALIVVGDTIHNFVDGILIAAAFLQGPELGLVTAVAIVAHEIPQEVGDFLVLLHS
                     GYAKSKAFVLNIASSLGSVVGGVVGYFALEGYQDWEPMLLGVVAASMIYVAVADLIPG
                     LHKRPELRDTAQQALLIAAGVGLIALVG"
     gene            3053..4774
                     /gene="phaC"
                     /locus_tag="AC16O9_07095"
     CDS             3053..4774
                     /gene="phaC"
                     /locus_tag="AC16O9_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HIR6516418.1"
                     /translation="MGTFQPQSTAAFLAAIAQSGNDAAKNWLRLAAGAPTPGAPADYL
                     ARFAADSERAAALQTEYLEKQRKLWQALAAGMKEAGVRAAPGDRRFAAREWRDDPYYS
                     YLRQSYLLAAEYLEQLVEASALEGQAKERLRFSVRQWVDAVSPANFAATNPEVLRLAQ
                     DTRGESLTRGLAQLIADAGRGRIGQTDESAFEVGRNLAVTPGTVIYENELMQLIQYAP
                     ATAEVAKRPLVMIPPCINKYYILDLQPENSFVRYAVGEGHTVFMVSWRNVGPEQGGYG
                     WDDYLEMGVLEALRTARAIARADRVNALGFCVGGTLLGAALAVLAAKGEHLVESATYL
                     TTMLEFSDTGQIGLFVDEASVAMREATLGGGGILPGRELATVFSALRANDLIWPYVVN
                     NYLKGGTPPAFDLLYWNADSTNLPGPMYCWYLRNTYLENKLRVPGAATNLGVPVDLGR
                     VDRPSFIVATREDHIVPWRSAYRSLEILGGEKTFVLGAAGHVAGVINPAAKKRRSYWA
                     DGPQDGDPETWLERAVEHPGSWWRRWAAWLEPYKGGTRKAPKKPGNARYEAIEPAPGR
                     YVKHKAN"
     gene            4792..5967
                     /locus_tag="AC16O9_07100"
     CDS             4792..5967
                     /locus_tag="AC16O9_07100"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6516419.1"
                     /translation="MEDIVIVGAARSAIGKFGGTLAKTPASDLGAHVMKQLLERSGVK
                     SEQVSEVILGQVLAAGVGQNPARQAAIKAGLPDMVPAMTINKVCGSGLKAVMLAAQSI
                     ANGDADLVVAGGQENMSIAPHVLNGSRDGFRMGDAKMIDSMIVDGLWDAFNQYHMGVT
                     AENVAKEYAVTREEQDEFAVKSQNKAEAAQKAGRFKDEIIPYEIKSKKGPVMFTDDEY
                     PRHGATLEAMKGLKPAFDKNGTVTAANASGINDGAAMVLVASAKKAKELGLKPIARIK
                     SFASAGVDPKYMGMGPVPASKRCLSRAGWEPKDLDLMEINEAFAAQAIAVNRQMGWDT
                     SKVNVNGGAIALGHPIGASGCRILVTLLHEMQKRDAKKGLASLCIGGGMGVALAVER"
     gene            5964..6755
                     /gene="phbB"
                     /locus_tag="AC16O9_07105"
     CDS             5964..6755
                     /gene="phbB"
                     /locus_tag="AC16O9_07105"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIR6516420.1"
                     /translation="MSAVLERENAAGEQARPAKARVAFVSGGMGGIGTAICRRLGAAG
                     HTVVAGCLPDYDKKAEWLAAMRGEGLRVHAAEGNVAEFDSCAEMFYHVKSVVGPVDIL
                     VNNAGITRDSLFKRMTEQDWMAVINTNLNSVFNVTRQVVDGMSERGWGRIVNISSVNG
                     IKGQFGQTNYSAAKAGMAGFSKALAQELVRKGVTVNTVSPGYVETELVKAIRTEIREQ
                     IVASIPMGRLAKPEEIAAVVAFLASEDAGYITGANISVNGGMHMM"
     gene            6791..7537
                     /gene="phbB"
                     /locus_tag="AC16O9_07110"
     CDS             6791..7537
                     /gene="phbB"
                     /locus_tag="AC16O9_07110"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIR6516421.1"
                     /translation="MAKQRLAVVTGGMGGLGESISTKMADAGYKVVVTYSPSNTKYKG
                     WLDEMKSQGYSFGAYPVDVADFDDCAAKVQRIAADVGAIDILVNNAGITRDMTFKKMG
                     KADWDAVMRTNLDSCFNMTKQVMDGMVERAWGRVINVSSVNGQKGAFGQTNYSAAKAG
                     IHGFTKALALEVAKKNVTINTISPGYIGTKMVTAIPKEILDSKILPQIPVGRLGKPDE
                     VAGLIIYLCSEEAAFVTGANISINGGQHMY"
     gene            7553..8152
                     /gene="phaR"
                     /locus_tag="AC16O9_07115"
     CDS             7553..8152
                     /gene="phaR"
                     /locus_tag="AC16O9_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR6516422.1"
                     /translation="MAAIPDDAKTDSRQRLIKKYPNRRLYDTKTSSYITLADVKQMVL
                     KQEEFRVVDAKSGEDLTRPILLQIILEEESGGVPMFSSDLLSQMIRSYGSAMQGFMGS
                     YLEKNLEAFQSMQKALQDQSQRIYGDNSRASQELWAQFMNLQGPAMQSLMQAYIEQSQ
                     KVFAQFQDQMQNQARSMFAGFSLPGFPAQPPGKTEPEKK"
     gene            8152..9438
                     /gene="rimO"
                     /locus_tag="AC16O9_07120"
     CDS             8152..9438
                     /gene="rimO"
                     /locus_tag="AC16O9_07120"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR6516423.1"
                     /translation="MVPKVGFVSLGCPKALVDSERILSRLRAEGYATAKDYGGADLVI
                     VNTCGFIDAAVEESLDAIGEALAENGKVIVTGCLGAKAGVVRSAHPSVLAVTGPHDTE
                     GVMRAVHAHLPKPHDPFADLVPPQGIKLTPRHYAYLKIAEGCNHRCSFCIIPSMRGDL
                     VSRPIGEVMQEAENLVKAGVKELLVISQDTSAYGVDVKYRAGFWQGRPLKTCMTALCR
                     SLGSLGVWVRLHYVYPYPHVDDVIPLMADGKILPYLDVPFQHASARILKLMKRPASAE
                     KVLERLSRWRDICPDLTVRSTFIVGFPGETEAEFEALLGFLEEAQLDRVGCFAYSPVD
                     GAAANALPDPVAEEIKEERRRRLMQAQEAISERKLKSKVGKSMQVLVDAPGVGRSAAD
                     APEIDGTVRFRGGRTGEFRQVTIERASAHDLHGRLT"
     gene            9435..10619
                     /locus_tag="AC16O9_07125"
     CDS             9435..10619
                     /locus_tag="AC16O9_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HIR6516424.1"
                     /translation="MSTREVVVLGGARTAIGDYGGSLKDFAPTKLGAIAIKEALTRAK
                     VDPATVGHVVMGSVIHGEARDMYLSRVAAIDAGLPVGTPCLTVNRLCGSGLQAIVSAA
                     QHILLGDTDTVVGGGAESMSRAAYFLPTGRWGQKMGDTWAVDAMTGALHDPFGHGHMG
                     VTAENIAARYGFTREQQDAFAIESHRRAANALEQGYFKEQVVPIEVKSKKGVEQVTRD
                     EHVRPDAKMEDLAKLKAVFKKDGTVTAGNASGINDAGAAIVMMEAGAAAKQGLKPRAR
                     LVAYAHAGVEPQVMGLGPIPAVKKVFEKAGLKPADMDVVESNEAFAAQAMAVTKDLGL
                     DPAKVNPNGGAVALGHPIGATGAILTVKALYELERTQKRYALVTMCIGGGQGIAAIFE
                     RI"
     gene            10782..11678
                     /gene="rssA"
                     /locus_tag="AC16O9_07130"
     CDS             10782..11678
                     /gene="rssA"
                     /locus_tag="AC16O9_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - phosphatidylcholine
                     lysophospholipase A1 activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HIR6516425.1"
                     /translation="MPSPRKPKIALALGAGGARGWAHIGAIRALLAQGIKPDIVCGTS
                     MGSLVGAAYAAGELERLEEWALSLGWRDVFGLMDFTLRGGLIRGKKLFEFMRARFGLR
                     DIGDLPMPYGAVATELASGREVWLRDGQVLDAVRASIAIPGVFAPVLRDGELLVDGAL
                     VNPVPVSMCRALDADIVIAVDLGWSKLGYYRELAQAQQAERAPGKAAWWSRFMPARAD
                     EALAESPDMPSAIGVFFASFDVMQVRVGRSRLAGEPADALITPILPDFAMMDFHRARE
                     AIDEGRAAAERARPVLEHLIGA"
     gene            11679..>12244
                     /locus_tag="AC16O9_07135"
     CDS             11679..>12244
                     /locus_tag="AC16O9_07135"
                     /inference="COORDINATES: protein motif:HMM:NF021472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HIR6516426.1"
                     /translation="MGQRDEERFAWPRVRRPASGALRRWFAAGWADLAAAPGPSLFYG
                     AALALMGLLLTRSFGGAVGIALTTGFLLVGPFLAIGLYELSRRRERGEPQRLAPSLVA
                     WRANFPAIGFYALALTLLLAVWIRVSVVVVALSFPEGRVDWGSPEAWGFAAAYAAAGG
                     ALALFVFATSSLSLPLLLDRRDMDTVSAA"
BASE COUNT         1980 a         4054 c         4402 g         1808 t
ORIGIN      
        1 agcgccctgt aggcctgcag catgatgaac agcgcctcct ccaccgccgg cgcctgcgga
       61 tagtccgcca ccgcggcctg tgcacggttg gcggcggcga caaaggcgcc gcgcttcatg
      121 tagtagcgcg ccacgtgcac ctcgtgacgc gcgagcgaat tgaccagata gcgcatgcgg
      181 gccgccgagt cctcggcgta cttcgagccg gggaagcgcg tcaccacctc gcggaacgcg
      241 gcgaaggcct cgcgcgccgc cttcggatcg cgctcggcga ggtccgggtc cgagaggatc
      301 gcgaacaggc cctggttctc ggtgaaattg acgaggccct tgaggtagta ggcgtagtcg
      361 agcgccgcgt ggttggggta gagcttgatg aagcgctcga cggcggcgat ggccgcggcc
      421 ggctcgtcga acttccagtg cgcccaggcg acgtcgagct gcgcctgctg cgcgtagggg
      481 ccgtaaggaa agcgcgcctc gagcttctcc aggtacttga tggctttcgc ccagtcgcgc
      541 tcccgcatgg cgtcacgcgc ctcgccgtgc aggcgctcga ccgaccagct ggcggtttca
      601 tcgggctcga ccggcgcggc gcagccggcg agcgccagcg cggcggcggc cagcagagca
      661 agtacacttg ttcgcatggg gcgcgattat acggacgcgg acataggttc cgaggggccg
      721 actgggacgc gagtttcgtc gcgccgatga gcgccgaggc gccgcgcgcg ctcgccgggc
      781 tgcggttgga ccaggcgctg gcgcgcatgt tcccgcaata ctcgaggagc cggctgcagg
      841 cctggctggc ggagggccac atcaccgtgg acggcgcgcg cgccacggcg cgcgcgctcg
      901 tgcggggcgg cgagcgcgtc gcgctctcgc cgcccgcggt gccgcagggc gcgccgcgcg
      961 cgcagcgcat gccgctcaag gtggcctacg aggacgcgga cctggtggtc atcgacaagc
     1021 ccgcgggcct cgtggtgcac ccgggcgccg gccagcccga ccgcacgctg atgaacgcgc
     1081 tccttgcgca cgcgccgcag ctcgccggcg tgccgcgcgc gggcatcgtg caccgcctcg
     1141 acaaggacac cacgggcctg atcgtggtgg cgaagacgct cgaggcgcaa gtgagcctcg
     1201 tgcgccagct cggcgagcgc agcatgcgtc gggtgtactg ggcggtggtg cagggcgatc
     1261 cgccagcgag cggcgtgatc gacgcgccgg tggggcgcga tgtgcgcgcg cgcacgcgca
     1321 tggcggtcac gcgccgcggc aaggacgcgg tgacgcgcta ccgcgtgctc gagcgcttcg
     1381 ggcgcgcggc gctggtcgag tgccgcctag agaccgggcg cacgcaccag atccgcgtgc
     1441 acttccagca catccgccat ccgatcatcg gcgaggcgac ctaccgccgc ggcacgcgcc
     1501 acggcatcgg cttcacgcgc caggcgctgc acgcggtgga gctctcgctc aagcatccgc
     1561 gaagcggcca gcccatgacc tggcgcgccg agccgcctgc cgacttccgc gggctgctca
     1621 agcgcctgcg caatgattcg ccctgactgg ccggcgccgc ccggcgtgcg cgcgctggtc
     1681 accacgcgcg gcgaaccaga cccgccgctc cccgcgccgc cggcctggct gcagcaggtg
     1741 cacggtacgc gcgtggtgga tgcggctgcc gcgcgcggga agccgcaggc cgatgccagc
     1801 gtcgccagag tgccgcacgc cgtctgcgcg gtgcaggccg cggactgctt gccggtgctc
     1861 ctggcggatg acgctggcgc cgtggtcggc gctgcgcacg cgggctggcg cgggctcgcc
     1921 gccggcgtga tcgaggccac cgtggccgag atgcaggttc ccggcgcgcg cctcatcgcc
     1981 tggctgggcc cggccatcgg ccccgcgcac tacgaggtcg gcgcggacgt gcgcaacgcg
     2041 ttcctcgcgc gcgacgccgc agcggcgcag gccttcgtgc cgaaccgccc cgggcactgg
     2101 ctgctcgacc tgtacgcggt ggcgcgccag cggctcgccg ccttgggcgt cgcgcgcgtg
     2161 tacggcgggg atttttgcac ctacagcgac ccgacgcgtt ttccctcgtg gcggcgcgag
     2221 cgcacgcgcg agcgcatcgc cgcgttcgtg tggttagaat cgcgctcccc atgacgctga
     2281 cttggatcgt gctggcgtgc ctcgcgggcg gcgtgctctc ggtcggcgcc gccgcgttcg
     2341 cgctggtgct gcgcgcgaaa tggatcccga tgctggtttc gttcgccatc ggcacgctgc
     2401 tcggcgcagc gttcctcgag gtcctgccgc acgcctttgc cgagggcgat gcgcgttcgg
     2461 tggcgacggc gatcctcggc ggcatcctcg gcttctttct gctggaaaaa ctcctgctgt
     2521 ggcgccactc gcacgagcac ggcgacgccg acggccctgc gcatcaccag gagcacgcgc
     2581 gcgccggggc gctgatcgtg gtcggcgaca cgatccacaa cttcgtcgac ggcatcctca
     2641 tcgccgccgc gttcctgcag gggccggagc tcggcctggt gaccgcggtg gcgatcgtcg
     2701 cgcacgagat cccgcaggaa gtgggcgact tcctggtgct gctgcattcg ggctacgcga
     2761 aatcgaaggc cttcgtcctg aacatcgcgt cctcgctcgg cagcgtggtc ggcggcgtgg
     2821 tcggctactt cgcgctcgag ggctaccagg actgggaacc gatgctgctc ggcgtggtcg
     2881 ccgcgagcat gatctatgtc gccgtggccg acctgatccc gggcctgcac aagcgtccgg
     2941 agctgcgcga cacggcgcag caggcgctgc tcatcgccgc gggcgtaggg ctgatcgcgc
     3001 tggtcggata agggtttgac cgtgggtatt tgtgcggcgc aaaatagcgc ccttgggtac
     3061 cttccagcca cagtccacgg ccgctttcct cgccgccatt gcccagtcgg gcaatgacgc
     3121 ggcgaagaac tggctgcgcc ttgccgccgg cgccccgacc ccgggcgctc cggccgatta
     3181 ccttgcgcgc ttcgcggccg acagcgagcg cgccgccgcg ctgcagaccg agtacctgga
     3241 aaagcagcga aagctctggc aggcgctcgc cgccgggatg aaagaggcgg gcgtgcgcgc
     3301 cgcccccggc gaccggcgct tcgcggcgcg cgagtggcgc gacgacccct attacagcta
     3361 cctgcggcag tcctacctcc tcgccgccga gtacctggag cagctggtcg aggcctcggc
     3421 gctcgagggc caggccaagg agcgcctgcg cttcagcgtg cgccagtggg tggacgccgt
     3481 cagcccggcg aatttcgccg ccaccaatcc cgaggtgctg cgccttgccc aggacacgcg
     3541 cggtgagagc ctgacccgcg gcctcgcgca gctcatcgcc gacgcggggc gcgggcgcat
     3601 cggccagacc gacgaatccg cattcgaggt cggccgcaac ctcgcggtca cgccgggcac
     3661 ggtgatttac gagaacgagc tgatgcagct gatccagtac gcgcccgcga ccgccgaggt
     3721 ggcgaagcgc ccgctggtga tgattccgcc gtgcatcaac aagtactaca tcctcgacct
     3781 gcagcccgag aactcgttcg tgcgctacgc cgtgggcgag ggccacacgg tgttcatggt
     3841 gtcctggcgc aatgtcggcc ccgagcaggg cggctacggc tgggacgact acctcgagat
     3901 gggcgtgctc gaggcgctac gcacggcgcg cgccatcgcg agagcggatc gcgtcaacgc
     3961 gctcggcttc tgcgtcggcg ggacgctcct tggcgccgcg ctcgcggtgc tggcggcgaa
     4021 aggcgagcac ctggtggaga gcgccaccta tctcaccacc atgctcgaat tttcggacac
     4081 cgggcagatc ggcctgttcg tcgacgaggc gagcgtcgcg atgcgcgagg cgacgctcgg
     4141 cggcggcggc atcctgcccg gccgggagct ggccacggtg ttctccgcgc tgcgcgccaa
     4201 cgacctcatc tggccgtacg tggtcaacaa ctacctgaaa ggcggcacgc cgccggcgtt
     4261 cgacctgctg tattggaacg ccgacagcac caacctgccc gggccgatgt actgctggta
     4321 cctgaggaat acctacctgg agaacaagct gcgggtgccg ggggcggcca ccaatcttgg
     4381 cgtgccggtg gacctgggcc gggtggaccg gccgagcttc atcgtcgcca cgcgcgagga
     4441 ccacatcgtg ccctggcgct cggcttaccg gtcgctggag attcttggcg gcgagaagac
     4501 gttcgtgctc ggcgccgccg gccacgtcgc cggcgtcatc aatccggcgg cgaagaaacg
     4561 ccgcagctac tgggccgacg gcccgcagga cggggaccct gagacctggc tcgagcgcgc
     4621 cgtggagcac ccgggaagct ggtggaggcg atgggctgcc tggctcgagc cgtacaaggg
     4681 cggcacgcgc aaggcgccga agaagccggg caacgcgcgc tacgaggcga tcgagccggc
     4741 cccggggcgc tacgtgaagc acaaggcaaa ctaggaaaca aggagaaggc catggaagac
     4801 atcgtcatcg tcggcgcggc aaggagcgcc atcggcaagt tcggcggcac gctggcgaag
     4861 acgccggcct ccgacctggg cgcgcacgtc atgaaacaac tgctcgagcg ctcgggcgtt
     4921 aagtcggaac aggtgtccga ggtgatcctt ggccaggtgc tcgccgcagg cgtcggccag
     4981 aacccggcgc gccaggcggc gatcaaggcg ggcctgccgg acatggttcc cgccatgacc
     5041 atcaacaagg tgtgcggctc gggcctgaag gcggtgatgc tcgccgcgca gtcgatcgcc
     5101 aacggcgatg ccgacctcgt ggtggcggga ggccaggaga acatgagcat cgcgccgcac
     5161 gtgctgaacg gctcgcgcga cggcttccgc atgggcgacg ccaagatgat cgactcgatg
     5221 atcgtcgacg gcctgtggga cgcgttcaac cagtaccaca tgggcgttac cgcggaaaac
     5281 gtggccaagg agtacgccgt gacgcgcgag gagcaggacg agttcgcggt gaagagccag
     5341 aacaaggccg aggccgcgca aaaggcgggg cgcttcaagg acgagatcat cccctacgag
     5401 atcaagtcga agaaaggacc ggtgatgttc accgacgacg agtacccgcg ccacggcgcg
     5461 acgctcgagg cgatgaaggg cctgaagccc gccttcgaca agaacggcac ggtgaccgcg
     5521 gccaacgcct cgggcatcaa cgacggcgcg gccatggtgc tggtggcgag cgcgaagaag
     5581 gccaaggagc tcggcttgaa gccgatcgcg cgcatcaagt ccttcgcctc ggcgggggtc
     5641 gatccgaagt acatgggcat gggcccggtg ccggcgtcca agcgctgtct gtcccgcgcc
     5701 ggctgggagc cgaaggatct cgacctgatg gagatcaacg aggccttcgc cgcccaagcc
     5761 atcgccgtca accggcagat gggctgggat acgtcgaagg tcaacgtgaa cggcggcgcc
     5821 atcgcccttg ggcaccccat cggcgcctcg ggctgccgca tcctggtcac gctcctgcac
     5881 gaaatgcaga agcgcgacgc gaagaaaggc ctggcctcgc tttgcattgg cggcggcatg
     5941 ggcgtggcgc tggcggtgga gcgatgagcg ccgtgctgga gcgggagaac gccgccgggg
     6001 agcaggcgcg gccggcgaag gcgcgcgtcg ccttcgtgag cggcggcatg ggcgggatcg
     6061 gcacggcgat ctgccggcgc ctgggcgccg cgggccacac cgtggtggcg gggtgcctgc
     6121 ccgattacga caagaaggcc gagtggctgg cggcaatgcg cggcgaaggc ttgcgcgtgc
     6181 acgccgccga aggcaatgtg gccgagttcg acagctgcgc cgagatgttc taccacgtga
     6241 aatcggtggt cggcccggtg gacatcctgg tcaacaatgc cggcatcacg cgcgacagcc
     6301 tgttcaagcg catgaccgag caggactgga tggcggtgat caacaccaac ctgaacagcg
     6361 tgttcaacgt cacacgccag gtggtcgacg gcatgagcga gcgcggctgg ggccgcatcg
     6421 tcaatatctc ctcggtgaac ggcatcaagg gccagttcgg gcagaccaac tactccgcgg
     6481 ccaaggccgg catggcgggc ttctccaagg cgctggcgca ggagctggtg agaaagggtg
     6541 tcacggtgaa caccgtatcg ccgggctacg tcgagaccga actggtgaag gcgatccgca
     6601 cggaaatccg cgagcagatc gtcgcctcga tcccgatggg gcgcctcgcg aagccggagg
     6661 agatcgcggc ggtggtcgcg ttcctggcct ccgaggacgc cggctacatc accggcgcca
     6721 acatttcggt gaacggcggc atgcacatga tgtgatggtg tagactcatt tcggaggagg
     6781 aaggagcatc atggcgaagc agcggcttgc cgtcgtgacc ggcggcatgg gcggtttggg
     6841 ggagtcgatc tcgacgaaga tggccgacgc tggctacaag gtggtggtga cctactcgcc
     6901 atccaatacc aagtacaagg gctggctcga cgagatgaag tcgcagggct acagcttcgg
     6961 cgcctacccg gtggacgtgg ccgacttcga cgactgcgcg gcgaaggtgc agagaatcgc
     7021 cgccgacgtc ggcgccatcg acatcctggt gaataacgcc ggcatcacgc gcgacatgac
     7081 gttcaagaag atgggcaagg cggactggga cgccgtgatg cgcaccaatc tcgactcgtg
     7141 cttcaacatg accaagcagg tgatggacgg catggtggag cgcgcctggg gccgcgtcat
     7201 caacgtgtcc tcggtgaatg gccagaaggg cgccttcggc cagaccaact attcggcggc
     7261 gaaggccggc atccacggct tcaccaaggc gctggcgctg gaggtggcga agaagaacgt
     7321 caccatcaac accatctccc cgggctacat cggcaccaag atggtgaccg cgatcccgaa
     7381 agagatcctc gactcgaaga tcctgccgca gattcccgtc gggcggctgg gcaagcccga
     7441 cgaagtggcg ggcctcatca tttacctgtg ctcggaagag gccgcgttcg tcaccggggc
     7501 gaacatctcg atcaacggcg gccagcacat gtactaagga gtagactccg ccatggccgc
     7561 gattccagac gacgccaaga ccgacagccg ccagcggctg atcaagaagt atcccaaccg
     7621 gcgcctgtac gacaccaaga cctcgagcta catcacgctc gcggacgtca agcagatggt
     7681 gctgaagcag gaggagttcc gcgtcgtcga cgccaagagc ggcgaggacc tgacgcgccc
     7741 gatcctgctg cagatcatcc tcgaggagga atcgggcggc gtgccgatgt tctcctcgga
     7801 cctcctgtcg cagatgatcc gctcctacgg cagcgccatg cagggcttca tgggctcgta
     7861 cctggagaag aacctggagg cgttccagag catgcagaag gcgctgcagg accagtcgca
     7921 gcggatttac ggcgacaact cgcgtgcctc gcaggagctg tgggcgcagt tcatgaacct
     7981 gcagggcccg gcgatgcaga gcctgatgca ggcctacatc gagcagagcc agaaagtgtt
     8041 cgcgcagttc caggaccaga tgcagaacca ggcgcgtagc atgttcgcgg gcttctccct
     8101 gccggggttc ccggcgcagc cgccggggaa gaccgagccc gaaaagaagt agtggttccc
     8161 aaggtcggct tcgtttccct gggttgcccc aaggccctgg tggattcgga gcgcatcctc
     8221 agccggcttc gcgccgaggg ctacgccacg gcgaaggact atggcggggc ggacctcgtc
     8281 atcgtcaaca cctgcggctt catcgacgcg gcggtcgagg agtcgctcga cgcaatcggc
     8341 gaggcgctgg cggaaaacgg caaggtgatc gtcaccggct gcctcggcgc aaaggcgggc
     8401 gtggtgcggt cggcgcaccc gagcgtgctc gcggtaaccg gcccgcacga caccgagggc
     8461 gtgatgcgcg cggtgcacgc gcacctgccc aagccccacg atccattcgc ggatctcgtg
     8521 cccccccagg gcatcaagct caccccgcga cactacgcgt acctgaagat cgccgaaggc
     8581 tgtaaccacc gctgcagctt ctgcatcatc ccgtcgatgc gcggcgatct cgtgtcgcgc
     8641 ccgatcggcg aggtgatgca ggaggcggag aacctggtca aggcaggggt gaaggagctc
     8701 ctcgtcatct cgcaggacac gagcgcgtat ggcgtggacg tcaagtaccg cgccggcttc
     8761 tggcaggggc ggccgctgaa gacatgcatg acggcgctgt gccggtcgct cggctcgctc
     8821 ggcgtgtggg tacggctgca ctacgtctac ccctacccgc acgtcgacga cgtgatcccg
     8881 ctgatggcgg acggcaagat cctgccgtac ctcgacgtgc cgttccagca tgccagcgcg
     8941 cggatcctga agctcatgaa gcgcccggcg agcgcggaga aggtgctcga gcgcctgtcg
     9001 cgctggcgcg atatctgtcc cgatctcacg gtaaggagta ccttcatcgt cggcttcccg
     9061 ggcgagaccg aggccgagtt cgaggcgctg ctcgggtttc tcgaggaagc gcagctcgat
     9121 cgcgtcggct gcttcgccta ctcgccggtg gacggcgcgg cggcgaacgc gctgcccgat
     9181 ccggtggcgg aagagataaa ggaggagcgc cgccgccgat tgatgcaggc gcaagaggcg
     9241 atcagcgaac gcaagctgaa atcgaaggtc ggaaagagca tgcaggtgct ggtcgatgcg
     9301 ccgggcgtag ggcgctccgc tgcggatgca cccgagatcg atggcacggt acgcttccgg
     9361 ggtggcagga cgggcgaatt caggcaggtc acgatcgagc gcgccagcgc gcacgaccta
     9421 cacgggagac tgacatgagc acacgcgaag tggtggtact gggcggggcg cgcacggcga
     9481 tcggcgatta cggcgggtcg ctcaaggact tcgcgccgac caagctcggc gcgatcgcca
     9541 tcaaggaggc gctcacgcgc gccaaggtgg acccggccac ggtcggccac gtggtgatgg
     9601 gtagcgtgat ccacggcgag gcgcgcgaca tgtacctgtc gcgcgttgcg gcgatcgacg
     9661 cggggctgcc ggtgggcacg ccgtgcctca cggtgaaccg gctttgcggc agcgggctgc
     9721 aggcgatcgt ctcggcggcg cagcacatcc tgctcggcga taccgacacg gtagtcggcg
     9781 gcggcgccga gagcatgagc cgcgcggcct acttcctgcc caccggccgc tggggccaga
     9841 aaatgggcga cacctgggcc gtggacgcca tgaccggcgc gctgcacgac ccgttcggcc
     9901 acggccacat gggcgtcact gcggagaaca tcgccgccag gtacggcttt acgcgcgagc
     9961 agcaggacgc gttcgcgatc gaatcgcacc ggcgcgcggc caacgcgctc gagcagggct
    10021 acttcaagga gcaggttgtc cccatcgagg tgaagtcgaa gaagggcgtc gagcaggtga
    10081 cgcgcgacga gcacgtgcgc ccggacgcga agatggagga cctggcgaag ctgaaggccg
    10141 tgttcaagaa ggacggcacg gtgaccgcag gcaacgcgtc cggcatcaat gacgcgggcg
    10201 cggccatcgt catgatggaa gccggcgccg cggcgaagca gggcttgaag ccgcgcgcgc
    10261 ggctggtggc ctacgcgcat gccggcgtcg agccgcaggt gatgggcctc gggccgattc
    10321 cggcggtgaa gaaggtgttc gaaaaggcgg gcctgaagcc cgccgacatg gacgtggtgg
    10381 aatcgaacga agccttcgca gcgcaggcca tggccgtcac gaaagacctc ggcctcgacc
    10441 cggcgaaagt gaacccgaac ggcggcgcgg tggcgctcgg ccacccgatc ggtgccaccg
    10501 gcgcgatcct caccgtcaag gcgctctacg agctcgagcg cacgcagaag cgctacgccc
    10561 tggtcacgat gtgcatcggc ggcgggcagg ggatcgcggc aatcttcgaa cgcatttaaa
    10621 tggggacaga cctcatttat ctttttttcg tgagccgcgg ggggcggcgc gcgatcgagg
    10681 ccagtgcctt gatttgattt tcattggtct cggcttttcc ggccggaaga ttcacccagc
    10741 cggaaaagcc gagatgtatt tgaaaggcga aaatccgcgg catgccaagc ccccgcaaac
    10801 ccaaaatcgc ccttgccctc ggcgccggcg gcgcgcgcgg ctgggcgcac atcggcgcga
    10861 tccgcgcgct gctcgcgcag ggcatcaagc ccgacatcgt ctgcggcacg tcgatgggct
    10921 cgctcgtggg cgcggcctac gccgctggcg agctcgagcg cctggaggaa tgggcgctgt
    10981 cgctcggctg gcgcgacgtg ttcgggctca tggacttcac gctgcgcggc ggcctgatcc
    11041 gcggcaagaa gctgttcgag ttcatgcgcg ctcgcttcgg gctgcgggac atcggggact
    11101 tgccaatgcc gtacggcgcg gtggcgaccg agctcgcttc cgggcgcgag gtctggctgc
    11161 gcgacgggca ggtgctcgac gcggtgcgtg cctcgatcgc cattcccggc gtgttcgcgc
    11221 cggtgctgcg ggacggcgag ctgctggtcg acggcgcgct cgtcaacccg gtgccggtct
    11281 ccatgtgccg cgcgctcgat gccgacatcg tcatcgccgt ggacctgggc tggtcaaagc
    11341 tcggctacta ccgcgagctc gcgcaggcgc agcaggccga gcgcgcgccc ggcaaggccg
    11401 cctggtggag ccgcttcatg ccggcgcgcg cggacgaagc cctggccgag agcccggaca
    11461 tgccgtcggc gattggcgtg ttcttcgcca gcttcgacgt gatgcaggtg cgcgtggggc
    11521 gcagccggct cgcgggcgag cccgccgacg cgcttatcac gccgatcctg cccgatttcg
    11581 ccatgatgga cttccaccgc gcgcgcgagg cgatcgacga ggggcgcgcg gcggccgagc
    11641 gcgcgcgccc ggtgctcgag catctgatcg gcgcctgaat gggccagcgc gacgaggagc
    11701 gcttcgcgtg gccgcgggtg cgccgtcccg cgtccggcgc gctgcggcgc tggttcgcgg
    11761 cgggctgggc cgatctcgca gcggcgccgg gcccgagcct gttctacggc gccgcactcg
    11821 cgctcatggg gctgctgctc acgcgctctt tcggcggtgc ggtcggcatc gcgctcacga
    11881 ccggcttcct gctggtgggc ccgttcctcg ccatcgggct gtacgagctc agccgccggc
    11941 gcgagcgcgg cgagccgcag cgcctggcgc cgtcgctcgt cgcgtggcgc gccaactttc
    12001 cggcgatcgg cttctacgcg ctcgccctga cgctgctgct cgcagtgtgg atccgggtct
    12061 ccgtggtcgt cgtcgcgctc tcgttcccgg aagggcgcgt cgactggggc tctcccgagg
    12121 cctgggggtt cgcagcggcc tacgccgcgg cgggcggcgc gctcgcgctg ttcgtgttcg
    12181 cgacctcgtc gctgtcgctc ccgctgctgc tcgaccggcg cgacatggac acggtcagcg
    12241 cggc
//
LOCUS       DBNHKE010000114        11543 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1395193, whole genome shotgun sequence.
ACCESSION   DBNHKE010000114 DBNHKE010000000
VERSION     DBNHKE010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1395193"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1208)
                     /locus_tag="AC16O9_07140"
     CDS             complement(168..1208)
                     /locus_tag="AC16O9_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516427.1"
                     /translation="MLKRFAVAVATVSPLVCIAADLAPAGTVGTLTVKVTVEGAGKEK
                     PGPGEAGTGTQWSVKNSGEFTARLRARDASADDSAENQQKQEALSGIVSQTLTEKDEE
                     VMNKWQDKEDACNGNEACENKVMMQKMSDPEFQRIQRKMQGAAPAAFAAGAQINMAPT
                     MQLWGLHDEGSGARLQINLTETIYGVDVEGESETKPGTYIKYKDDLTCRLSGSPRVGA
                     GSFHHDVLLRVDGKRQTYEVRIPGDGIFVELQSVCTLKQGRPSRSLTGHSWTDRAGPI
                     RIPLMGRPKSESADSSRAEFNELLTFKGKVASASNPRLSGKRSFAADLRGYRIAAGPV
                     KVTVEWQFVGGK"
     gene            1610..2230
                     /locus_tag="AC16O9_07145"
     CDS             1610..2230
                     /locus_tag="AC16O9_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6516428.1"
                     /translation="MDRDVECARIVASTASPAFAIDEAGRIAAWNPEAEQFFGFGAAN
                     VVGRFCWDTVGGLDPYLNLYCGPHCALLEMARQRRAIHACELLFRNAEGKRRRAHVSS
                     FIVAGRSPAEMVIVHFLQLAPHDATGENYRLNGDGTETGNPLLTERQLQVLRLMAHGH
                     TTREIARELRISVGTVRNHCGKILQALQAHTRLQAVDAARALHMLG"
     gene            2450..3523
                     /locus_tag="AC16O9_07150"
     CDS             2450..3523
                     /locus_tag="AC16O9_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HIR6516429.1"
                     /translation="MKRTILSVAIGSLLALPAIATYAQNCEIDAGNLPKDVQCTKTRE
                     QVRAELIAAQRAGEVIVNAELGTTAGAVSTQTAGMTRDQVRAELLAAHRAGEVIVNAE
                     LGTTAGPMSTQTARMARSQALAAVPAFATDNYEIDAGNIPGEVSSSMTREQVRAELIA
                     AQRAGDVIVNAELGTTAGAMSTQTAAMTRDQVRAELLAAQRAGEHMINAELGAQAGQL
                     YARAGETYPVSATSPHYVLINLGSASNDLAVYPSQMTLKAGVVYQFVVSNPSTDTHVL
                     AAPELAASANTTELTRLSTWPQRAAERIVDPTATLSKGIWVLPGQVLEWTFTPTKVGS
                     YKFGCESRAHSAAGMFSTVTVTL"
     gene            complement(3675..3827)
                     /locus_tag="AC16O9_07155"
     CDS             complement(3675..3827)
                     /locus_tag="AC16O9_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516430.1"
                     /translation="MRAPRSLGAIPLVAGVDGDRANDLLLLDDARAEVMFQSAPGVFL
                     PPRALH"
     gene            complement(3891..4115)
                     /locus_tag="AC16O9_07160"
     CDS             complement(3891..4115)
                     /locus_tag="AC16O9_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR6516431.1"
                     /translation="MPIFEYGCRKCGREFETLVRSNAAPACPQCSSTDLEKKLSVFAT
                     ARADGNAEALAAGPCGTCGHPGGPGACALN"
     gene            complement(4211..6196)
                     /locus_tag="AC16O9_07165"
     CDS             complement(4211..6196)
                     /locus_tag="AC16O9_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516432.1"
                     /translation="MKSLAQKVLVLSLGALLLLASLYVLVTREMDSWNDASVQVMRDY
                     KRAMLDGELHAALTRATGETASYVLTGEDRYRAEAAEAIERAEVAVGSLRQVASEPRH
                     ITEDDTHLRFLERQERMLGVTRERFARATVAAPRMDAAATAEALSEIYANEEETDALW
                     EEIADHHRTEQVNNVAIVQDHSVRARILFLGGAAAFAFAAILLIGYVRRRVVRPLTLL
                     ARLTQSVAAGDPTARAEVTHADEIGQLQHSFNKMVADLEQQRRQVAGLVEGLEVSRDA
                     ARRASRAKSDFLANVSHEIRTPMNGVLIGLDLLHETASSPEQREVADMARAGARHLLG
                     MLNDLLDFSRIETGRLKLESVAFEPRKLVTQMVELHGRRAAEKGVAMSCQIAGDVPEK
                     LCGDPTRLGQILLNLLHNAIKFTDRGSIEVSVSLEEPQAGPAAPGAAQPPLWLRFRVT
                     DTGVGITAEAADKIFEPFYQAGGESIHASAGIGLGLGIARELARLMGGALGFESEAGK
                     GSSFWFTARLLPEAECSAATAAEAPPSRLQPGKSILLVEDHRDTREVMTRMLQRRGLR
                     VTTAGSGGDALSLATTGDFDLILMDCRMPEMDGFEATRAIRAAAGDRARVPIVALTAY
                     GLTEPKQHYLDSGFDDLVVKPYTLEDIEAMLQRWLAR"
     gene            6658..6942
                     /locus_tag="AC16O9_07170"
     CDS             6658..6942
                     /locus_tag="AC16O9_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516433.1"
                     /translation="MFNTHLEQRLLAPNLPATRPRDTIAWPPYPVGTPTPYMVFTLLY
                     GFTDAWTMRPAMVLNMRFLGNTTGEKTRLPGNGGLWPSDHAALAARLRFD"
     gene            7223..8863
                     /locus_tag="AC16O9_07175"
     CDS             7223..8863
                     /locus_tag="AC16O9_07175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6516434.1"
                     /translation="MQGTDSTRSTADRSGRREEFRLLRYFTLTSFIALAVAGLALFLL
                     ERMEIAFFQEVQREQQAFFAKAQADLAREQEAAARRSLLAVHEAGHVNLTKAFSNMLW
                     RSDLAAFVAQVQAMPVDQCRAAAAEAREACFAQLRARIMALPGFAALDAKIRATARDS
                     TVFKVKVYDLRGITAYSSEHAQIGEDKADNDGWKRAARGTPASELAHRDQFSAFERVV
                     ANRDVISSYIPGRAPGSDAVAGVFEIYSDATPFVAQIRGVAARQAALAAENQATVERA
                     AAMNEDRVHINAYAVLAVVGGLLALLYLALYLVARRGQRILDRQAREREQSIRREQQW
                     HREKMDALATMAANVSHEVGNPLSTITMLARDMAQAQGGAPGEEPRVILEEAQRIARM
                     TREISSFAAARSESPELLDVNAAVKAMCEFLSYDWRARATRIDFQPGAQLPACSVVPD
                     HLNEALMGLLQMCAQDAPDQPAATPRVLRVRTEARGAGVAIRIGLEPAAALHAKPGAG
                     EDAELRFEAALRRVQGMGGRLEWRDGTIEVILPPAPGA"
     gene            8979..10529
                     /locus_tag="AC16O9_07180"
     CDS             8979..10529
                     /locus_tag="AC16O9_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516435.1"
                     /translation="MLELLDWGKKQGEEAAGSGSGPKPAQAAPAVEGDAGTALAELNA
                     RLEGVADAADPKARIGALGAIQDAGAAQVAALLGQYLGGTAGTHAAREVLWNSLAGYQ
                     TRLAQALCAAAGAALTLASATRALRALRVLAKLHLVHYAGMPGKLWRVAYAIHAGAER
                     AGFAATPVRVRADEHTMTTVEQELLRLLMLRVSAPDMMAPEQIEIADRVIEELGAEFT
                     LRPPGVADNAFCFEPGSEFAPRRAQGRAPGTSARYFGPGMGHDSLKRIARQLTAAGRD
                     GFKAYGKDIAPGVQQSAVQHLLLFWRDDCPYAPPAHASASGTLQVVHGYDTLWQHLSR
                     SRQGPAALSLAEWSPAAAQPPETWPLRGEGGNELAAEVPAASRAWAKCGAVVGVSSGE
                     DARWVGMVRRMHAQADGSLRADLAVLSRAPRALSLREVVELGDDSAFTNASSRQFAVS
                     GVNAVILAEGAGDAQPANLLVPAAAWKEGRVYETSEDPPRQLRTVQLVRRGDDFVRAT
                     FEWLTAPA"
     gene            11164..>11543
                     /locus_tag="AC16O9_07185"
     CDS             11164..>11543
                     /locus_tag="AC16O9_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516436.1"
                     /translation="MSTQVVSGVAATGAPLAGEVTLRDASPEYREKVVVIAGDGGFSV
                     DVSDMRAPYVLRAVGAAEGRRHALYSLADAPGLANINPLTSAALANAAGVDDPADVFE
                     GRDPARLERARLGMPAAIAALGLKL"
BASE COUNT         1788 a         3994 c         3980 g         1781 t
ORIGIN      
        1 ggcatggaca tgccgtccat ggtcatcttg gcggtgatct cccagagctc gtccgccccc
       61 tgggcgagcg cggacgatgc agaaaagcac aggcaggcgg ccagcacggc cggcgcggcg
      121 cgacggatca tcttggttct ccttttctcc ggatcagacg ggcggcgcta cttgccgccg
      181 acgaactgcc actcgaccgt caccttgacg ggcccggcgg cgatgcggta acccctcaag
      241 tcggcggcaa agctgcgctt tccgctgagg cgcggattgc tcgccgaggc gaccttgccc
      301 ttgaaggtca acagctcatt gaactccgcg cggctgctgt ccgcgctctc gctcttgggc
      361 ctgcccatca aggggatcct tatggggccc gcgcggtccg tccacgagtg gcccgtaagg
      421 ctgcggctgg gtcggccctg cttcagcgtg cagacggatt gcagctctac gaagatgcca
      481 tcgcctggaa tgcggacctc gtaggtctgg cgctttccgt cgacgcgcag gaggacgtca
      541 tgatgaaagc tccccgcgcc gacccgcgga ctgccgctca aacggcatgt caggtcatcc
      601 ttgtacttga tataggtgcc gggcttggtc tcggattctc cctcgacgtc gacgccgtaa
      661 atcgtctccg tcaggttgat ctgcaggcgc gcgcccgacc cctcgtcgtg caacccccac
      721 agttgcatgg tcggcgccat gttgatctgc gccccggccg caaatgctgc cggtgccgcg
      781 ccctgcatct tgcgctggat gcgctggaac tccgggtcgg acatcttctg catcatcacc
      841 ttgttctcgc aggcctcgtt gccgttgcag gcatcctcct tgtcctgcca cttgttcatg
      901 acctcctcgt ccttttcggt gagggtctgg gagacgatgc cgctcaaggc ctcctgcttc
      961 tgctggtttt ccgcggaatc gtccgccgag gcgtcccggg cgcggagacg ggcggtgaac
     1021 tcaccagagt tcttgactga ccactgggtc cccgtcccgg cctcaccggg tcctggcttt
     1081 tccttccccg ccccctcgac ggtgaccttg acggtaagag tgcccacggt gccagccggc
     1141 gcaaggtcgg cggcgataca gacgagcggg gatacagtcg cgaccgcaac cgcgaaacgc
     1201 ttcagcatag ttcgcaggcc tccctagacc ctgcgtgccg aatggtaatg cacagaatgc
     1261 gcttcaagca caggctgttt tgacgacgcc cggccagctt tcggatccgg cggccgcccg
     1321 ccacgacctc gccatcgtgg gcgcgtgctt ctcgggccgt cacgtgctgc gctgtaggcg
     1381 cgggcttcgc gtgccgccgg tccgtcgccg cccccggccg aactcgttcc atggcgcggg
     1441 ccgcgacgaa gttcctatgc ctaaggggtc attgccgggg gcacgtagcg cacgccgtgc
     1501 cctccagccg gtggcgcgac atgtctttgc gctaagtcaa cacgccaaga gctccctggg
     1561 tataaatagg aaaattaaga cccacgaatc ttgggctacg ggcggccgca tggaccggga
     1621 cgtggaatgt gcacgaatcg tcgccagcac ggcgagcccg gcgttcgcca tcgacgaagc
     1681 gggacggatt gccgcgtgga atccggaggc cgagcaattc tttggttttg gcgcagccaa
     1741 tgtcgtaggc cgcttctgct gggatacggt cggtggactg gatccctatc taaacctgta
     1801 ctgtggccca cactgcgcgt tgctcgaaat ggcgcggcag cggcgagcga tccatgcttg
     1861 cgagctgctc tttcgcaatg ccgaaggcaa gcgccggcgc gctcacgtca gctcgtttat
     1921 cgtcgcaggg cgatccccgg cggagatggt tattgtccac ttccttcagc tggcgccaca
     1981 cgacgcgacg ggcgagaact atcgcctcaa cggtgacggt acggagaccg gaaatcccct
     2041 gctcacggag cggcagcttc aagtgctgcg tcttatggcg cacggccata cgacccggga
     2101 aatcgcccgc gagctgagga tcagcgtcgg gacggtgcgc aaccactgcg gcaagatcct
     2161 gcaggcgctg caggcgcaca cgcgactgca agcggtggac gcagcgcgcg cgctgcacat
     2221 gctcggctga ttttcgcccc cggcgggaga tcgtgcgcgc atgcgcatgt gccgatttgc
     2281 acatgtgctc tatggcaact gccgctggca agagccaagc cgggcgggcg ccgctgcgcc
     2341 atttggctag cccgagttgc gccgtttggc gcgtttccgg cgtcgcgaaa gaggagtaga
     2401 tctgtaggcg atgcagtaca gcctcagacc gaagctaagg agccacgcca tgaaacgcac
     2461 aatcctctca gttgcgatcg gcagcctgct ggcgctgccg gccatcgcga catacgcaca
     2521 gaactgtgaa atcgatgcgg gcaaccttcc caaggatgtc cagtgcacca agacgcgcga
     2581 gcaggtgcgc gccgagctga tcgccgcgca acgggcgggc gaggtgatcg tcaacgcgga
     2641 gctgggcacg accgccggcg ccgtgtcgac gcagaccgcg ggcatgacgc gcgaccaggt
     2701 gcgtgccgag ctgctcgccg cccaccgcgc gggcgaggtg atcgtcaacg cggagctggg
     2761 cacgaccgcc ggccccatgt cgacgcagac ggcgcgtatg gcgcgttccc aggcgctagc
     2821 cgcggtgccg gccttcgcca ctgacaacta cgaaatcgac gcgggcaaca ttcccgggga
     2881 ggtctcgtcc agcatgacgc gcgaacaggt gcgcgccgag ctgatcgccg cccaacgggc
     2941 gggcgacgtg atcgtcaacg cggagctggg cacgaccgcc ggcgccatgt cgacgcagac
     3001 cgcggccatg acgcgcgacc aggtgcgcgc cgagctgctc gccgcgcaac gggcgggcga
     3061 gcacatgatc aacgccgaac tgggcgccca agccggccag ctctatgcaa gggctggtga
     3121 aacgtatccc gtcagcgcga cgtctcccca ctacgtgctc atcaacctgg ggtcggcatc
     3181 caacgacctg gccgtttacc cttcccagat gacgctgaag gcgggggtgg tctatcagtt
     3241 cgtggtcagc aatccaagca cggacacgca cgtgttggca gcgcccgagc tcgcggcaag
     3301 cgcaaacacc acggagttga caagactgtc aacatggccg caacgggccg cagaaagaat
     3361 cgttgacccc accgcgaccc tcagtaaggg tatttgggtg ctgccaggcc aagtgttgga
     3421 gtggacgttt acgccgacaa aggtaggcag ctacaagttt ggatgcgaaa gccgcgccca
     3481 ctcagcggca ggaatgttct cgacggtaac cgtgacgctt tgatcgcgct tcgagcggga
     3541 tcagataggc gggtccgatc cgggcgccgg ccctcctcag ggggccggcg ccgcctgcct
     3601 gtccaggaag gctcgacgcg gcaaggcaag gccgaagacg ggattcgcaa gttccaggtt
     3661 gcgattgccg gaactcaatg cagcgcgcgg ggcggcagga acacgccggg cgcggactgg
     3721 aacatcacct ccgcacgcgc gtcatcgagc aagaggaggt cgtttgccct gtcgccgtcg
     3781 acaccggcca ccaggggaat cgcgccaagc gagcgaggcg cacgcatccg tcgactcccg
     3841 ccgcgagact gcgccgggcg tgcgcctcgc gcaggtcgcg ccgcaagaat tcagttcagc
     3901 gcgcaggcgc cgggcccgcc cggatgcccg caggtcccgc acgggcccgc ggccagcgcc
     3961 tcggcgttgc cgtccgcgcg cgcggtcgcg aacaccgaga gcttcttttc caggtcggtg
     4021 gagctgcatt gcgggcaggc cggggcggcg ttcgagcgca ccagcgtttc gaattcccgc
     4081 ccgcacttcc tgcagccgta ctcgaagatc ggcatcgcgt gctcctttcg gcgcgcattg
     4141 tgcagcacaa gagacatcgt cggccactcc ggccgcgcgc ctgccccggc cggcctgccg
     4201 ccgccccggg ctaccgcgcc agccaacgct gaagcatcgc ctcgatgtcc tccagcgtgt
     4261 agggcttgac caccaggtcg tcgaacccgc tgtcgaggta gtgctgcttc ggctcggtca
     4321 gcccataggc ggtgagcgcg acgatcggca ccctggcgcg gtcgcccgcg gccgcgcgga
     4381 tggcgcgcgt cgcctcgaat ccgtccatct ccggcatgcg gcaatccatc agaatcagat
     4441 cgaagtcccc cgtcgtggcg aggctgagcg cgtcgccccc gctcccggcc gtggtcacgc
     4501 gcaggccccg gcgctgcagc atgcgcgtca tgacctcgcg ggtgtcgcga tggtcctcga
     4561 ccagaagaat cgacttgccc ggctgtagcc gcgagggggg cgcttcggcc gccgtcgccg
     4621 cgctgcactc ggcttcaggc aacaggcgcg cggtgaacca aaagctcgat cccttgcccg
     4681 cctcgctttc gaagccgagc gcgccgccca tcaatcgcgc cagctcgcgc gcgatcccca
     4741 ggcccaggcc gatgcccgcg ctcgcgtgta tcgattcgcc gccggcctgg tagaacggct
     4801 cgaaaatctt gtcggctgcc tcggccgtga tgccgacgcc ggtgtccgtg acgcgaaagc
     4861 ggagccacag cggcggctgc gccgcgcccg gcgcagccgg cccggcttgc ggttcctcga
     4921 gcgacaccga gacttcgatc gatccccggt cggtgaactt gatggcgtta tggagaaggt
     4981 tcagcaggat ctgacccagt cgcgtcgggt cgccgcacag cttctccggg acatccccgg
     5041 cgatctggca gctcattgcc acccctttct cggcagcgcg cctgccgtgc agctcgacca
     5101 tctgggtgac gagcttgcgg ggctcgaacg cgaccgattc caacttcagc cggcccgtct
     5161 cgatgcggga aaaatcgagc aggtcattga gcatgccgag cagatggcgc gcgccggcgc
     5221 gcgccatgtc ggcgacctcg cgctgctccg ggctcgaggc ggtttcgtgc agcagatcca
     5281 ggccgatgag aacaccgttc atcggtgtgc ggatttcgtg gctcacgttg gccaggaaat
     5341 ccgacttggc gcggctcgcc cgccgcgcgg catcgcgcga gacctccagg ccttcgacca
     5401 gccccgcgac ttggcggcgc tgctgctcga gatccgccac catcttgttg aagctgtgct
     5461 gcagctggcc gatttcgtct gcgtgtgtca cctcggcgcg agccgttgga tccccggccg
     5521 cgaccgactg ggtcagtcgc gccagcagcg tgagcgggcg gaccacgcgc cgccgcacgt
     5581 agccgatcag caagatcgcg gcgaaggcga acgcggccgc gccgccaaga aaaaggatgc
     5641 gagcgcgcac gctgtgatct tgcacgattg ccacgttgtt cacctgctcg gttctgtgat
     5701 ggtcggcgat ctcctcccaa agcgcgtcgg tttcctcctc gttggcatag atctcgctca
     5761 gcgcctcggc cgttgccgcg gcgtccatgc ggggcgcggc gacggttgcc cgagcgaatc
     5821 gttctcgcgt cacacccagc atgcgttcct gccgctccag gaagcgaagg tgcgtgtcgt
     5881 cttcggtgat atgacgcggc tcgctggcga cctgtcgcaa cgaccccacc gcgacctcgg
     5941 ctcgctcgat cgcttcggcg gcttcggccc gatagcggtc ctcgccggtg agcacgtagc
     6001 tcgccgtctc gcccgtagcg cgggtcaatg ccgcgtgcag ttcgccatcc agcatcgcgc
     6061 gcttgtagtc gcgcattacc tggaccgagg cgtcgttcca cgagtccatc tcgcgcgtca
     6121 ccaggacgta gaggctggcc agcagcagca gcgcgcccag cgacaacacg aggactttct
     6181 gggcgaggct cttcatcgcg gcttgcccga tgcgcggcgg cgcgcaggct ggtcccggag
     6241 cgtcgagggg tcgtgcatgc gggcattttg catgcaggat cgtcatttgc catagcgccg
     6301 gttcgaacgc cggtgcgcgg gcgccgcgcg gccgcgcgcc tgagcggctc ggccaataca
     6361 gtgcgcctcg ctggcccgtc gcatcccccg gccaagcttg gcgcacaggg caggccgaat
     6421 ggaacttcgt aacccccttg ccgtggatca agcggctccg gcggcagggg tgtacaagct
     6481 cgaatgccac cccttcccgg gcgttccgcc cgcgccgggg atgggatgcg acgatccctc
     6541 gatccgggct gcaacttgca gacggcgccg ccgccactca gcacgccctt ggggctcatc
     6601 gccgtcgagc gcggtttcct cgccgtcgac gttacggtca ggaatcggcc ctagcgcgtg
     6661 ttcaacaccc acctggagca acgcttgctc gcgccgaacc tgcccgcgac gcgcccgcgg
     6721 gacaccattg cgtggccgcc gtacccagtg ggcacgccca cgccctacat ggttttcacc
     6781 ttactgtacg gcttcaccga tgcctggacg atgcgcccgg ccatggtgct gaacatgagg
     6841 ttccttggca atacgacggg cgagaagacg cggctgccag gcaacggcgg gctctggccg
     6901 tcggatcatg cggctttggc ggcgcggctg agattcgact gagcgcgcga ggagcgttgc
     6961 tgctggagaa cttcgccaat gccgagttga gcatggccgc catgcaacgc gagacgattc
     7021 gcagtttcct tccctgactg cggcgcgcag cggcccgccg cggcgatgcc cttcgcgcgg
     7081 cgatcgcccc ttcgcgcctc ggccctgcag tcccaaatcc atgccgtcgc caccggtgcg
     7141 agtccccgcc cggtatcatc gccgctgggg aactccccga cagcgctcgc gcgtggcccc
     7201 ggaaggagcg gatcagcaga gcatgcaggg cacggactcg acgcgctcca cggcggaccg
     7261 ctccgggcgg cgggaggagt ttcgcctgct gcgctacttc acgctcacca gcttcatcgc
     7321 gctggccgtt gccgggctcg cgctctttct cctcgagcgc atggagatcg cgttcttcca
     7381 ggaggtgcag cgcgagcagc aggcgttctt cgcgaaagcg caggccgatc ttgcgcgcga
     7441 gcaggaggcg gcggcccgcc gcagcctgct ggcggtgcac gaggcgggcc acgtcaacct
     7501 cacgaaagca ttctcgaaca tgctgtggcg atccgacctc gccgccttcg tcgcgcaagt
     7561 gcaggcgatg ccggtcgatc agtgccgcgc ggcggccgcc gaagcccgcg aggcgtgctt
     7621 cgcgcagctg cgcgcgagga tcatggcgct ccccggcttc gccgcgctcg atgccaagat
     7681 ccgcgcgacg gcgcgcgaca gcaccgtgtt caaggtcaag gtgtacgacc tgcgcggcat
     7741 cacggcctac tcctccgagc acgcgcagat cggcgaggac aaggccgaca acgacggctg
     7801 gaagcgcgcg gcccgcggca cgccggcgag cgagctggcc caccgcgacc agttcagcgc
     7861 gttcgagcgc gtggtcgcca accgcgacgt tatctcgagc tacatcccgg ggcgcgcccc
     7921 gggcagcgac gcggtcgccg gcgtgttcga gatctactcg gacgcgacgc cgttcgtcgc
     7981 gcagatccgg ggcgtcgccg cgcggcaggc ggcgctcgcc gccgagaacc aggcgacggt
     8041 cgagcgcgct gccgcgatga acgaggaccg ggtccacatc aacgcgtacg cggtcctcgc
     8101 ggtcgtcggc gggctgctcg cgctgctgta cctggcactc tatctcgtcg cgcggcgcgg
     8161 ccagcgcatc ctcgaccgcc aggcacgcga gcgcgagcag tcgatccggc gcgagcagca
     8221 gtggcaccgc gagaagatgg acgcgctcgc gacgatggcg gccaacgtct cgcacgaggt
     8281 cggcaacccg ctctcgacga tcaccatgct cgcgcgcgac atggcgcagg cgcagggcgg
     8341 cgcgccgggc gaagagccgc gcgtcatcct ggaggaggcc cagcgcatcg ccaggatgac
     8401 gcgcgagatc tccagcttcg cggccgcgcg cagcgaatcg cccgagctgc tcgacgtcaa
     8461 cgccgcggtg aaggcgatgt gcgagttcct gagctacgac tggcgcgcgc gcgcgacgcg
     8521 catcgatttc cagccgggcg cgcagctgcc cgcctgcagc gttgtccccg accacctgaa
     8581 cgaggcgctg atggggctgc tgcagatgtg cgcgcaggac gcccccgatc agccggcggc
     8641 gaccccgcgg gtcctgcggg tgcgcaccga ggcgcgcggc gcgggcgtgg cgatccgcat
     8701 tggcctcgag cctgcggccg cattgcacgc gaaaccgggc gccggggagg acgcagagct
     8761 gcgcttcgaa gccgcgctgc gccgggtgca aggcatgggc gggcgcctgg agtggcgcga
     8821 cgggacgatc gaggtgatcc tgccgccggc ccccggggcc tagcgcgctg cgttactcgc
     8881 cataacgccg gcccgcgcgg tgacaaagtg catgccgacg ggcgcggcgc cggcctagaa
     8941 tgagcgccta ggggggagca cgggtacgga gggcggcgat gctggagctg ctggattggg
     9001 gcaagaagca gggcgaagag gccgccggct ccggatccgg tccgaagccg gcgcaggcgg
     9061 cgccggccgt cgagggcgac gcgggcaccg cgctcgccga gctgaacgcg cggctggaag
     9121 gcgtggccga tgccgcagac cccaaggcgc gcatcggcgc gctcggcgcg atccaggacg
     9181 cgggggcggc gcaggttgcc gcgctcctcg ggcaatacct cggcggcacg gcgggcacgc
     9241 atgccgcgcg cgaggttctg tggaattcgc tcgccggcta ccagacgcgc ctggcgcagg
     9301 cgctgtgcgc ggcggccggc gccgcgctca ccctggcgag cgcgacgcgc gcgctgcgcg
     9361 ccctgcgcgt gctcgccaag ctgcacctcg tgcactacgc gggcatgccc ggaaagctct
     9421 ggcgcgtggc ctacgccatt cacgcgggcg ccgagcgcgc gggcttcgcg gcgacgccgg
     9481 tgcgcgtgcg cgccgacgag cacaccatga ccaccgtcga gcaggagctg ctgcggctgc
     9541 tcatgctgcg cgtgagcgcg cccgacatga tggcgcctga gcagatcgag atcgccgacc
     9601 gggtcatcga ggagctgggc gcggagttca cgctgcgccc gccgggggtc gccgacaacg
     9661 cgttttgctt cgagccgggg agcgaattcg cgccgcggcg tgcgcagggc cgcgcgccgg
     9721 gcacgagcgc gcgctacttc ggccccggca tgggccacga ctcgctcaag cgcatcgccc
     9781 gccagctgac cgcggccggc cgcgacggct tcaaggccta cggcaaggac atcgcgccgg
     9841 gcgtgcagca aagcgcggtg cagcacctgc tcctgttctg gcgcgacgac tgcccctatg
     9901 cgccgccggc gcacgcgagc gcctccggta cgctgcaggt ggtgcacggg tacgacacgc
     9961 tctggcagca cctgtcgcga tcgcgccagg gcccggccgc gctgtcgctc gccgaatggt
    10021 cgcccgcggc ggcgcagccg ccggagacct ggccgctgcg cggcgaaggc ggcaacgagc
    10081 tcgccgccga ggtgccggcg gcgagccgcg cctgggcgaa gtgcggcgcc gtggtcggcg
    10141 tgtcctcggg cgaggacgcg cgctgggtgg gaatggtgcg ccgcatgcac gcgcaggccg
    10201 acggcagcct gcgcgcggac ctggcggtgc tgagccgggc gccgcgcgcg ctgtcgctgc
    10261 gcgaagtggt ggagttgggc gacgacagcg cgttcaccaa cgcctcctcg cggcaattcg
    10321 ccgtgagcgg cgtgaacgcg gtgatcctcg ccgagggcgc cggcgacgcg cagcccgcga
    10381 acctgctcgt ccccgccgcc gcctggaagg aaggccgcgt ctacgagacg agcgaagatc
    10441 cgccgcgcca gctgcgcacc gtgcagctgg tgcggcgcgg cgacgacttc gtgcgcgcca
    10501 ccttcgagtg gctgacggcg ccggcctagc agctgcggca tcctggcgat catgccggcg
    10561 gcggtgaggg ccgggccggc aaccggcggc gcgttctgcg gcgccgccat cagtccagcc
    10621 gatcctcgcc cagccgttca cgttgcgtgg cgcgcggcgc aaggaatttc ccgtggttca
    10681 tgttcgacag gcagtcctca ggaaaaaatc atcgtgttct cgtgcaagcg tcgcgcccgc
    10741 gcgtgcatca ggcatgaaag tgcatcgcgc gtgaacgtgc atcgcgcgcg aacttgcatc
    10801 gcgcgcgaac ttgcatcgcg cacgcacatg cgcgcaccta tgtaattccg aaacgtcgcc
    10861 gcacggcgac acaattcgtt cgctcgcgct tgatgctcgt caagacgcgc gcgaagtcgc
    10921 tccacacaat cgagtcacga gcacggcaat cgtgcgcatg ttcggctcgc gacgcgcggg
    10981 cgcaggtgtg tggatgcacc agcaccaaca gaacaggaga gcgagatgaa tccgactcca
    11041 atttcagaat tgcggctgcg gcttggttcg gccgccgcat tcgcatgcgc gcttgcgctg
    11101 ggcgccctcg tcgccggttg cggcggcggc ggcgacggtg cgggcacgaa tcagcaaggc
    11161 agcgtgtcaa cgcaggtcgt cagcggcgtg gcggccaccg gcgcgccgct cgcgggtgaa
    11221 gtcaccttgc gggacgcttc cccggaatac cgggagaagg tggtcgtcat cgcgggcgac
    11281 ggcggcttct cggtggatgt ctcggacatg cgggcgccgt acgtgttgcg tgcggtgggc
    11341 gcggcggagg gcaggcgaca cgcgctgtac tcgcttgcag acgcgccggg cctcgccaac
    11401 atcaacccgc tcacgagcgc ggcgctggcc aacgccgccg gcgtggacga ccccgccgac
    11461 gtcttcgagg ggcgcgatcc ggcgaggctg gaaagggcgc gcctgggcat gccggcggcc
    11521 atcgccgcgc tgggcctgaa gct
//
LOCUS       DBNHKE010000115        12819 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1395295, whole genome shotgun sequence.
ACCESSION   DBNHKE010000115 DBNHKE010000000
VERSION     DBNHKE010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1395295"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="AC16O9_07190"
     CDS             <1..402
                     /locus_tag="AC16O9_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6516437.1"
                     /translation="YREDPDATETNLTRAELTREREMVLKLTYGGELRVVRTSGSGLR
                     VGRDKENDLVVESHFASRAHARIYPREGHFVLQDLSSNGTYLLGDGTTTEILLRREEA
                     VLTERGWIGLGNSAARHGDHTLRFRVEPESD"
     gene            437..1678
                     /gene="glyA"
                     /locus_tag="AC16O9_07195"
     CDS             437..1678
                     /gene="glyA"
                     /locus_tag="AC16O9_07195"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6516438.1"
                     /translation="MSAADLLARTDPELWDAISLENRRQEDHVELIASENYVSPAVLA
                     AQGSALTNKYAEGYPGRRYYGGCEYVDLAETLAIERAKRLFGAEWANVQPHSGSQANQ
                     AVYFAALKPGDTVLGMSLAHGGHLTHGSPVNLSGKLYRFVSYGLDAKEVIDYDQAEKL
                     ALEHRPRLIVAGASAYSLAIDWKRFRQIADRCGALFMADMAHYAGMVAVGLYPTPVGV
                     ADYVTSTTHKTLRGPRGGFVIGNGDLGKAINSTVFPGLQGGPLMHVIAAKAAGFGEAL
                     KPEFKVYQQHVLDNAKAMVKVLLERGVRIVSGRTESHMMLLDLRSKKLTGKEAEAALG
                     HAHITVNKNAVPNDPEKPMVTSGIRIGTPAMTTRGFRVGEAEKLAHLIADVLDKPGDE
                     TLLRRVAGEVRALCSAHPVYA"
     gene            1688..2152
                     /gene="nrdR"
                     /locus_tag="AC16O9_07200"
     CDS             1688..2152
                     /gene="nrdR"
                     /locus_tag="AC16O9_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR6516439.1"
                     /translation="MRCPFCGHDDTQVVDSRSSTEASAIRRRRKCPSCDKRFTTYERV
                     DLKIPRLVKKDGSRVEFDREKLAGSMKIALRKRPVATEALEAAIVKIEDRLRALGERE
                     VPTSRVGDLVMRELAKLDKIAYIRFASVYRRFETPEDFREALQEVKAKKRRK"
     gene            2149..3219
                     /gene="ribD"
                     /locus_tag="AC16O9_07205"
     CDS             2149..3219
                     /gene="ribD"
                     /locus_tag="AC16O9_07205"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR6516440.1"
                     /translation="MSTFSELDHAMMRRALALAEKGLYTATPNPRVGCVLVKGEQVIG
                     QGWHEKTGGPHAEARALLQASAAAKGATAYVTLEPCNHHGKTPPCAEALIKAGVTRVV
                     AAMRDPNPESKKGGDALLAARIPFEAGLLEEEARELNVGFVSRMTRARPWVRMKIAAT
                     LDGRTALSNGVSQWITGPEARKDGHRWRARACAILTGAGTVRADDPLLTVRDVETPRQ
                     PLRVIADSHLETPPNARILQGEKVLIFAARDGTAPPNAEVVVLPNPQNKVELKKMLKE
                     LALRGVNELHVEGGFKLNGSLVREGCVDEFLIYLSPSFLGDSAQGMVNLAEVTSLDDR
                     LRVEIRSVERIGDDLRILARPA"
     gene            3219..3791
                     /locus_tag="AC16O9_07210"
     CDS             3219..3791
                     /locus_tag="AC16O9_07210"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR6516441.1"
                     /translation="MFTGIVHAVGKIVSVRPFVVDAGKLPLAQVKVGDSIAVQGACLT
                     VTKKRGKRLSFDVSQETLDCTVGLDRPGNVNLEKAMRLSDRLGGHLVSGHVDGVGLLL
                     RVEGSVYRFRAPKAVARYVAPKGSVCIDGVSLTVNRVSGAEFEVNLIPHTLKVTTLSR
                     LKPGAEVNMEADLVARYVEGLAKAAQGVQR"
     gene            4043..4372
                     /locus_tag="AC16O9_07215"
     CDS             4043..4372
                     /locus_tag="AC16O9_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516442.1"
                     /translation="MAHKKQQALDPSIKENASVLSDCTSAVFALSPDDIGRISAMDTG
                     PDKDPKITRYALLVNDDTYDSARQFSDQVNKGGKSVIIFTSLEVASLWLGLDASTVRE
                     LIAGADG"
     gene            4469..4666
                     /locus_tag="AC16O9_07220"
     CDS             4469..4666
                     /locus_tag="AC16O9_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516443.1"
                     /translation="MPPEDVADQILQAVESDKRELILAKGAEYEIPQLRRKDPDLLFE
                     RMSAMVSEGYARRLAADKTRG"
     gene            complement(4678..5289)
                     /locus_tag="AC16O9_07225"
     CDS             complement(4678..5289)
                     /locus_tag="AC16O9_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6516444.1"
                     /translation="MTGPTKDFWEERFSTGNTPWDRGASSPQLGAWLASGALQPCRIL
                     VPGCGSGHEVAELAMAKFEVTGLDYADEAIARTHRRISEAEVDAAIVRADVLAWEPDR
                     PFDAIYEQTCLCALYPDQWREYADRLYQWLRPGGRLFALFLQLPRAEAAQGFIQGPPY
                     HCDINGMRALFPESRWDWPKPPYPRTTHPAGIAELAVVLERTG"
     gene            5322..8939
                     /gene="hrpA"
                     /locus_tag="AC16O9_07230"
     CDS             5322..8939
                     /gene="hrpA"
                     /locus_tag="AC16O9_07230"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR6516445.1"
                     /translation="MQIRFDPALPVSERRKDIAAAIAAHPVTIVCGETGSGKTTQLPK
                     ILLEMGRGERRQIGHTQPRRIAARSVAARIAEELQVELGTLVGYKVRFTDKVGRSTRV
                     KLMTDGILLAETQGDPDLQGYDTLILDEAHERSLNIDFLLGYVKRLLPRRPDLKVVIT
                     SATIDAERFSKQFDGAPVIEVSGRLYPVEIRYRPVGGDEEDTTRDEEEQALGDAVEEL
                     FREGPGDVLVFLPGEREIRDAAGVLAKRNLKGAEILALYGRLSVAEQDRVFHPGGARR
                     VVLATNVAETSLTVPRIRYVVDAGQARVKRYSYRNKVEMLRVENISQAAAQQRAGRCG
                     RVANGVCIRLYGEDAFKARPAYTDPEVLRASLASVILRALSLNLGKVEDFPFLDAPNS
                     RAIADGYALLAELGAVDEDNALTGLGRELARLPLDPRVARMLVAARAEGCLEQMLVVA
                     AALSVQDPRERPLAKQSAADERHAKFADERSDFLALLKLWQLQGEAGLRRLCRDNFLS
                     YPRMREWRDVHQQLGAVTEEMDWKASSARMDKPEGFRAVHRALLAGLLGNIGMKDEAD
                     GNYTGARGIKFWVHPGSHTKRPGKWLVAAELVETTRLYARTVATIDVQWLEELGAHLL
                     KRHRDNPHWEKNRAQVVAVERGTLYGLPVYTNRRVHYGPLDPELSRKLFIRSALVEGE
                     FVTKAPFFAHNQRLVTEIERMEHKQRRPDILVDDELIYAFYDARIPPGIHNGADFEKW
                     RKEAEHGNPRLLHLKREDLMRHEAAGITTDNFPHALRLGANEFRLEYHFDPRSPRDGV
                     TLTVPVALLNQVPQARCEWLVPGLLKEKVRALAKSIPQRLRNKLGPLDEFAADFAARA
                     RPADAPLAQALARHVRAELNLEIPLDAFRPDSAPPHLHMNFLVVDEHGRQLGQGRNLA
                     QLKHDLAAHTTEVLQKEAPLEEGEHYTGWTVGDLAEMMEIERGGQTLVGYPALVDAGD
                     GVTLQVFDTPERAAELHRAGVRRLIAIAFKERVRDLERAWTKDMALAPLKDDLVTAAI
                     DRAFLAERVPTLAAEFARAVDAGRSRFMLIAQEVARTAAGLLAAQADLKKKLAPAAKS
                     FPQAAQDIEQQLARLLGSGWLANTPWARLQHYARYLKAIELRLEKLRADPARDARLMS
                     DLQPLLTAWLRAPRPLDAEREQFGWLLEELRVSLFAQQLKTPVPVSAKRLAKLWQSIR
                     R"
     gene            8936..9676
                     /locus_tag="AC16O9_07235"
     CDS             8936..9676
                     /locus_tag="AC16O9_07235"
                     /inference="COORDINATES: protein motif:HMM:NF018917.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HIR6516446.1"
                     /translation="MNSITNSLAYALANILHPRMIWLMIWPLVIALGLWGTLAMVFWA
                     QLALALAEWMQSGLAHAPLIGSWDFTTATLFLAKVLILVMLVPLIQLTALLILSVFGM
                     PSMVEHVASRSFTRLERRKGGSLAGSVWNSSVGLAGLVGLGIASIPLWVFPPLWPLIP
                     VAILAWVNQRVLRYDALAEHAAADEMRRLFSARWGGLYVMGLVLALLAYIPILGFFAP
                     VLLGVSFIHYLLGALEEQRGAPIEGQVV"
     gene            9673..10428
                     /locus_tag="AC16O9_07240"
     CDS             9673..10428
                     /locus_tag="AC16O9_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR6516447.1"
                     /translation="MTGFGAFVFGDEILVGKRQDKHFPWLIGALGKRGLRLAWCEHLG
                     DEPARVTQALKRTFATQDVVFCFGGIGATPDDHTRQCAAAALGVELALHPEAEAEIRG
                     RFGAETNPQRLKMGEFPAGAQIIPNPFNRIPGFSVGHHYFVPGFPQMAWPMIEWVLDE
                     KYRQLFDSQRWAEGSLIVYDLPESAVAPLMEELNRDFPGLKAFSLPSMGADGTRRHIE
                     LGVRGAQDEIAPAMERLRRGVAELGGALDVPKR"
     gene            10477..10728
                     /gene="rpsP"
                     /locus_tag="AC16O9_07245"
     CDS             10477..10728
                     /gene="rpsP"
                     /locus_tag="AC16O9_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6516448.1"
                     /translation="MVVIRLARGGANKRPFFNLVVADSRRAAKGKFIERVGFYDPKAP
                     EGREALRVNVERLKHWQGKGAQLSTTVKRLVKQFGKKAA"
     gene            10739..11206
                     /gene="rimM"
                     /locus_tag="AC16O9_07250"
     CDS             10739..11206
                     /gene="rimM"
                     /locus_tag="AC16O9_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR6516449.1"
                     /translation="MGRVAGSYGVRGWVKVAPQAGVQESLAAAKQWWLDEAPRRVEAA
                     RVHSATVIAKLEGVESREQALALKGRVVSVARAALPRAEAGHYYLADLLGLQVVNTQG
                     VALGVVRQWLTNGPQDVMELAGDQVRLVPWVAAVVKNVDLEAKVIEVEWEADW"
     gene            11206..11952
                     /gene="trmD"
                     /locus_tag="AC16O9_07255"
     CDS             11206..11952
                     /gene="trmD"
                     /locus_tag="AC16O9_07255"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR6516450.1"
                     /translation="MRFDVVTLFPEMFAAVTASGIPRKALEAGLWRLATWNPRDFTTD
                     NYRTVDDRPYGGGPGMVMLAEPLEKALDAAKAAGGGAVLYLSPQGRRLDHAKVMELAG
                     RGAVTLLCGRYEGVDERLIRRRVDEELSLGDFVLSGGELAAMAVIDAVVRQLPGALGD
                     GQSAAEESFAAGLLDCPQYTRPEVYAGEKVPEVLLSGHHEQIRRWRLQQALGRTWLRR
                     PELIAARQLSGEEHKLLEEFRQQAKERSER"
     gene            11949..12335
                     /gene="rplS"
                     /locus_tag="AC16O9_07260"
     CDS             11949..12335
                     /gene="rplS"
                     /locus_tag="AC16O9_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6516451.1"
                     /translation="MNLIETIEKEEIKRLGKTIPDFSPGDTVVVNVTVVEGERKRAQA
                     FEGVVIAKKNRGIGSSFTVRKISSGEGVERTFQTYSPLIASIEVKRKGEVRRAKLYYL
                     RQRSGKSARIREKVGGASAAGEGGEE"
     gene            12499..>12819
                     /locus_tag="AC16O9_07265"
     CDS             12499..>12819
                     /locus_tag="AC16O9_07265"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR6516452.1"
                     /translation="MRRELTAISPVADIVADLRAGKMVVLVDDEDRETEGDLVFAAEF
                     VTPEKVNFLARHGRGLICMPITEAHALRLGLRPMVEQNRSRHGTNFTVSIEAAEGIAT
                     GISAA"
BASE COUNT         2104 a         4241 c         4566 g         1908 t
ORIGIN      
        1 taccgcgagg accccgacgc gacggagacc aacctgacgc gcgccgagct cacgcgcgag
       61 cgcgagatgg tgctgaagct cacctacggc ggcgagctgc gcgtggtgcg cacctccggc
      121 agcggcctgc gcgtcgggcg cgacaaggag aacgacctcg tggtggagag ccatttcgcc
      181 tcgcgcgcgc acgcccgcat ctacccgcgc gagggacact tcgtgctgca ggacctgtcg
      241 tccaacggga cctacctcct cggcgacggc accaccaccg aaatcctgct gcggcgtgaa
      301 gaagccgtgc tcaccgagcg cggctggatc ggcctcggca atagcgccgc gcgccacggc
      361 gaccatacgc tgcggtttcg cgtcgagccg gagtcggact agcctgtaga atcgggcgct
      421 ttcgcacttg ccccccatgt ccgccgccga cctcctcgcc cgcaccgacc ccgagctctg
      481 ggacgccatc agcctggaga accgccgcca ggaggaccac gtcgagctga ttgcctcgga
      541 gaactacgtc agcccggcgg tgctcgccgc gcagggctcg gcgctcacca acaagtacgc
      601 cgagggctac ccgggccggc gctattacgg cggctgcgag tatgtggacc tggccgagac
      661 gctcgccatc gagcgcgcca agcggctgtt cggcgcggag tgggccaacg tgcagccgca
      721 ctcgggctcg caggccaacc aggccgttta cttcgcggcg ctcaagcccg gcgacacggt
      781 gctcggcatg tcgctcgcgc acggcgggca cctcacgcac ggctcgcccg tcaacctctc
      841 cggcaagctc taccgcttcg tatcctacgg gctggacgcg aaggaagtca tcgattacga
      901 ccaggccgag aagctcgcgc tcgagcaccg gccgaggctg atcgtcgccg gtgcctcggc
      961 gtactcgctg gccatcgact ggaagcgctt tcggcaaatc gccgaccggt gcggggccct
     1021 cttcatggcc gacatggcgc actacgcggg catggtggcg gtcggcctgt atccgacgcc
     1081 ggtgggtgtc gccgattacg ttaccagcac cacgcacaag accctgcgcg ggccgcgcgg
     1141 cggcttcgtc atcggcaacg gcgatctggg caaggcgatc aacagcacgg tgttcccggg
     1201 cctgcagggc gggccgctga tgcacgtcat cgccgccaag gcggccggct tcggcgaggc
     1261 gctcaagccc gagttcaagg tttaccagca gcacgtcctg gacaacgcca aggcgatggt
     1321 gaaggtgctg ctcgagcgcg gcgtgcgcat tgtctcgggg cgcaccgagt cgcacatgat
     1381 gctgcttgac ctgcgctcga agaagctgac cggcaaggag gcggaagccg cgctcgggca
     1441 cgcgcacatc acggtgaaca agaacgcggt gccgaacgat cccgagaagc cgatggtgac
     1501 ctcgggcatc cgcatcggca cgccggcgat gacgacgcgg gggttccgcg tcggcgaggc
     1561 cgagaagctc gcgcacctga ttgccgacgt gctcgacaag cccggcgacg aaacgctgct
     1621 gaggcgcgtg gccggcgagg tgcgcgcgct gtgcagcgcg cacccggtgt acgcctgaga
     1681 tagcgcgatg cgctgcccgt tctgcggcca cgacgatacc caggtggtcg attcgcgctc
     1741 gagcacggaa gcgagcgcga tccggcggcg gcgcaagtgc ccgtcctgcg acaagcgctt
     1801 caccacctac gagcgcgtcg acctcaagat cccgcgcctc gtgaagaagg acggcagccg
     1861 cgtcgagttc gaccgcgaaa agctcgccgg cagcatgaag atcgcgctgc gcaagcgccc
     1921 ggtcgcgacc gaggcgctgg aggctgcgat cgtcaagatc gaggaccggc tgcgcgcgct
     1981 cggcgagcgc gaggtgccga cctcgcgcgt tggcgacctg gtgatgcgcg agctcgccaa
     2041 gctcgacaag atcgcctaca tccgcttcgc ttccgtctac cggcgcttcg agacgccgga
     2101 ggatttccgc gaggcgctgc aggaagtgaa ggcgaagaag cggcgcaagt gagcaccttc
     2161 tccgagctcg accacgccat gatgcggcgt gcgctcgcgc tggcggagaa ggggctgtat
     2221 acggcgaccc ccaatccccg cgtcggctgc gtcctggtaa agggcgagca ggtcatcggc
     2281 cagggctggc acgagaagac cggcgggccg cacgccgagg cgcgcgcgct gctgcaggcg
     2341 agcgccgcgg cgaagggcgc caccgcctac gtcacgctgg aaccctgcaa tcaccacggc
     2401 aagacgccgc cgtgcgccga ggcgctgatc aaggccggcg tgacgcgcgt ggtggcggcg
     2461 atgcgcgatc cgaatccgga gtcgaagaag ggcggcgacg cgctgcttgc cgcccggatt
     2521 ccgttcgagg ccgggctgct cgaggaggag gcgcgcgagc tcaatgtcgg cttcgtttcg
     2581 cgcatgacac gcgcccggcc gtgggtgcgg atgaagatcg ccgcgacgct ggacgggcgc
     2641 actgccctga gcaatggcgt gtcgcagtgg atcacggggc ccgaggcgag gaaggacggg
     2701 caccgctggc gcgcccgggc ctgcgccatc ctcaccggcg ccgggaccgt gcgggccgac
     2761 gatccgctgc tgaccgtgcg cgacgtggaa acgccgcgcc agcctttgcg cgtgatcgcc
     2821 gacagtcatc tggagacgcc gccgaatgcg cgcatcctgc agggcgagaa ggtgctcatc
     2881 ttcgcggcgc gcgacgggac ggcgccgccg aacgcagaag tcgtggtgct gcccaatccc
     2941 cagaacaagg tggagctgaa gaagatgctg aaggagctgg cgctgcgcgg cgtcaatgag
     3001 ctgcacgtcg agggcgggtt caagctgaac gggtcgctgg tgcgcgaggg ctgcgtggac
     3061 gagttcctga tctatctgag tccgagcttc ctcggcgatt cggcgcaggg catggtcaac
     3121 ctggcggaag tgacctcgct cgacgaccgg ctgcgggtag agatcaggtc ggtcgagcgg
     3181 atcggcgacg acctcagaat cctggcgcgg cccgcctgat gttcaccggc atcgtccatg
     3241 cggtcggcaa gatcgtcagc gtgcgtccgt tcgtggtgga tgccggcaag ctgccgcttg
     3301 cgcaggtgaa ggtgggggat tcgatcgccg ttcagggcgc ctgcctgacg gtgaccaaga
     3361 agcggggcaa gcgcttgtca ttcgacgttt cccaggagac gctggattgc accgtcgggc
     3421 tggatcgtcc tggcaacgta aacctggaga aggcgatgcg gttatcggat cggcttggcg
     3481 gtcatctggt gagcgggcac gtcgacgggg tggggttgct gctgcgggtg gaggggagcg
     3541 tgtacaggtt ccgtgcgccg aaggcggtgg cgcgctatgt cgcgcccaag gggtcggtgt
     3601 gcattgacgg ggtgagcctg acggtgaacc gggtaagcgg ggcggagttc gaggtgaatc
     3661 tgattccgca tacgttgaag gtgaccacgc tgtcgcggct gaagccgggg gcggaggtga
     3721 acatggaggc ggatctggtg gcgcggtatg ttgaagggtt ggcgaaggct gcacaggggg
     3781 tgcagcgcta gccgcgtcac ggatcggaag caggtatttg gatctgtgat cccgggattg
     3841 ggcgagtgtc cgcagaggca ggcttgcagc gtcgaaagct tgttcctgca tgcacatgcg
     3901 ttctttgccg agcgtccatc gcctgatcac tggtcaggcc gttttgagag aggtgcaacg
     3961 catgccctgc aagtccgtga tcgcgcagga cgggaaggtc gtggtagagc gatggacagg
     4021 cacggtctct cgccaggagc tcatggcgca caagaaacag caggcgctcg atccatcgat
     4081 caaggaaaat gcatcggtgt tatcggattg cacgagcgcc gtgttcgcgc tttcgcccga
     4141 cgacattggc aggatctccg caatggacac gggtccggac aaggacccga agatcacgcg
     4201 ctacgctttg ctggtcaacg acgacaccta cgacagcgcg cgccaattct ctgatcaggt
     4261 aaacaagggc ggaaagagcg tcattatctt cacttccctg gaagtcgctt cgctgtggct
     4321 gggccttgac gcgtcgacgg tgcgcgagct gatcgccggc gctgacggct agagtgtccg
     4381 gcgcgcctcg gcctggccac gaggcgcaaa cgccgacgca gggaagacgt cgaagcgcgg
     4441 agtcagcgac aaggcgatcg acaacggcat gccccccgag gacgtggccg accaaattct
     4501 gcaggccgtc gagtcggaca agcgcgagct gattctcgcg aaaggcgcgg agtacgagat
     4561 tccgcaactg cgtcggaaag atcccgactt gctgttcgag cgcatgtcgg cgatggtgag
     4621 cgagggctac gcccgtaggc tggccgccga caagaccagg ggctgaatcg tccgtcccta
     4681 gcctgtgcgc tcgagcacca ccgccagctc cgcgatcccc gccggatgcg tggtgcgcgg
     4741 atacggcggc ttgggccaat cccagcgcga ctcgggaaag agcgcgcgca tgccgttgat
     4801 gtcgcagtga tacggcgggc cctggatgaa gccttgcgca gcttccgccc gcggcagctg
     4861 caagaacagc gcgaatagtc tcccgcctgg ccgcagccac tggtacagcc ggtccgcgta
     4921 ctcgcgccac tggtctggat agagcgcgca caggcaggtc tgctcgtaga tggcgtcgaa
     4981 cggccggtcg ggctcccacg cgagcacgtc ggcccgtacg atcgccgcgt cgacctcggc
     5041 ctcgctgatc ctgcggtgcg tacgcgcgat cgcttcgtcg gcgtaatcga gccccgtgac
     5101 ctcgaacttc gccatggcga gctcggcgac ctcgtggcct gagccgcagc ccggcacgag
     5161 gatgcggcag ggctggagcg cgcctgaggc aagccacgcg ccgagctgcg ggctggatgc
     5221 gccccggtcc cacggcgtgt ttcccgtaga gaagcgctcc tcccagaagt ccttggtcgg
     5281 ccccgtcatg tgagcgccaa tcctatatcc tcgggcgcca catgcagatc cgattcgacc
     5341 cggcgctccc ggtcagcgaa agacgcaagg acatcgccgc ggccatcgcc gcgcacccgg
     5401 taactatcgt gtgcggcgag accggctcgg gcaagaccac gcagctgccg aagatcctgc
     5461 tcgagatggg gcgcggcgag aggcggcaga tcggccacac gcagccgcgc cgcatcgccg
     5521 cgcgctcggt cgcggcgcgc atcgccgagg agctgcaagt cgagctcggc acgctcgtcg
     5581 gctacaaggt gcgcttcacc gacaaggtgg gcaggagcac ccgcgtgaag ctgatgaccg
     5641 acggcatcct gctcgcggag acgcagggcg accctgacct ccagggctac gacaccctca
     5701 tcctcgacga agcgcacgag cgcagcctca acatcgattt cctgctcggc tacgtaaagc
     5761 gcctgctgcc gaggcggccc gacctcaagg tggtgatcac gtcggcgacc atcgacgccg
     5821 agcgcttctc gaagcagttc gacggcgcgc cggtcatcga agtctcgggc cggctctacc
     5881 cggtcgagat ccgctaccgc ccggtgggcg gcgacgagga ggacaccacg cgcgacgagg
     5941 aggagcaggc gctcggcgat gcggtggaag agctgttcag ggaaggcccg ggcgacgtgc
     6001 tcgtcttcct gcccggcgag cgcgagatcc gtgacgcggc gggagtgctc gcgaagcgca
     6061 acctgaaagg cgccgagatc ctggcgctct acgggcgcct gtcggtggcg gaacaggatc
     6121 gcgtgttcca cccgggcggc gcgcggcgcg tggtgctcgc caccaacgtc gccgagacct
     6181 cgctcacggt gccgcgcatc cgctacgtgg tcgacgcggg ccaggcgcgc gtcaagcgct
     6241 actcctaccg caacaaggtg gagatgctcc gcgtcgagaa catctcgcag gccgcggcgc
     6301 agcagcgcgc cgggcgttgc gggcgcgtcg ccaacggcgt ctgcatccgc ctgtacggcg
     6361 aggacgcgtt caaggcgcgc cccgcgtata ccgacccgga ggtgctgcgc gcctcgctcg
     6421 cgtcggtgat cctgcgcgcg ctttccctca atctcggcaa ggtcgaggac tttcctttcc
     6481 tcgacgcccc caattcacgc gccatcgccg acggctacgc gctccttgcc gagctgggcg
     6541 cggtggacga ggacaacgca ctgacaggcc tcgggcgcga gctcgcgcgc ctgccgctcg
     6601 atccgcgcgt cgcgcgcatg ctcgtcgccg cgcgcgccga aggctgcctg gaacaaatgc
     6661 tcgtcgtcgc cgccgcgctc tcggtgcagg acccgcgcga gcggccgctc gccaagcagt
     6721 ccgccgccga cgagcgccac gcgaaattcg ccgacgagcg ctcggacttc ctcgcgctgc
     6781 tcaagctctg gcagctccag ggcgaggcag gactgcggcg cctgtgccgc gacaatttcc
     6841 tttcctaccc ccgcatgcgc gagtggcgcg acgtgcacca gcagctcggc gcggtgaccg
     6901 aggagatgga ctggaaggcc tccagcgcgc gcatggacaa gcccgagggc tttcgcgccg
     6961 tgcaccgcgc gctcctcgcg ggcctgctcg gcaacatcgg catgaaagac gaggccgacg
     7021 gcaactacac cggcgcgcgc ggcatcaagt tctgggtgca cccgggctcg cacacgaaga
     7081 ggccgggcaa gtggctggtc gcggcggagc tggtcgaaac cacgcgcctt tacgcgcgca
     7141 ccgtggcgac gatcgacgtg cagtggctgg aggagctcgg cgcgcacctg ctgaagcgcc
     7201 accgcgacaa cccgcactgg gaaaagaacc gcgcccaggt ggtggcggtg gagcgcggca
     7261 cgctctatgg gctccccgtg tacacgaacc ggcgcgtgca ctacggcccg ctcgatccgg
     7321 aactgtcgcg caagctgttc atccgctcgg cgctggtgga gggcgagttc gtcacgaaag
     7381 cgccgttctt cgcgcacaac cagcgcctgg tgaccgagat cgagcgcatg gagcacaagc
     7441 agcgccggcc cgacattctc gtggacgacg agctgatcta cgccttttac gacgcgcgca
     7501 ttccgccggg catccacaac ggggccgact tcgaaaagtg gcgaaaagag gccgagcacg
     7561 gaaaccccag gctcctccac ctcaagcgcg aagacctgat gcgccacgag gccgcgggca
     7621 tcaccaccga caacttcccg cacgcgctgc ggctcggcgc gaacgagttt cggctcgagt
     7681 atcacttcga cccccgctcg ccgcgcgacg gcgtgacgct caccgtgccg gtggcgctcc
     7741 tcaaccaggt gccgcaggcg cgctgcgagt ggctggtgcc gggcctgctg aaggaaaagg
     7801 tgcgcgcgct ggcgaagtcc atcccgcagc gcctgaggaa caagctgggg ccgctggacg
     7861 agttcgccgc ggacttcgcc gcaagagcgc ggcccgccga cgcgccgctc gcgcaggcgc
     7921 tcgcgcgcca cgtccgtgcc gagctcaatc tggaaattcc gctcgacgcc ttccggccgg
     7981 attccgcccc gccgcacctg cacatgaact tcctcgtcgt cgacgagcac ggccgccaat
     8041 tgggccaggg tcgcaacctc gcgcagctca agcacgacct cgccgcgcac accaccgaag
     8101 tgctgcagaa ggaagcgcca ctggaggaag gcgagcacta cacgggctgg acggtgggcg
     8161 atctggccga gatgatggag atcgagcgcg gcgggcagac gcttgtcggc tacccggcgc
     8221 tcgtcgacgc gggcgacggc gtgacgctcc aggtgttcga cacgccggag cgcgccgcag
     8281 agctgcaccg cgccggagtg cgccggctca tcgccatcgc cttcaaggaa cgcgtccgcg
     8341 acctcgagcg cgcctggacg aaggacatgg cgctcgcgcc gctgaaagac gacctggtga
     8401 cggcggcgat cgaccgcgcc tttctcgccg agcgcgtgcc cacgctcgcc gccgagtttg
     8461 cgcgcgccgt ggacgccggg cgcagccggt tcatgctcat cgcgcaggaa gtggcgcgca
     8521 ccgcggccgg cctgcttgcc gcgcaggcgg acctgaagaa aaagctcgcg ccggccgcga
     8581 agtcctttcc gcaggcggcg caggacatcg agcagcagct cgcgcggctg ctcggcagcg
     8641 gctggctggc gaataccccc tgggcgcgcc tgcagcacta tgcgcgctac ctcaaggcga
     8701 tcgaactgcg gctggagaag ctgcgcgccg acccggcgcg cgacgcgcgt ctgatgtcgg
     8761 acctgcagcc gctgctcacg gcgtggctgc gcgcgccgcg cccgctcgat gcggagcgcg
     8821 agcagttcgg ctggctgctg gaagagctcc gggtgtcgct ctttgcgcag cagctgaaga
     8881 cgccggtgcc cgtttcggcc aagcgcctcg ccaaattgtg gcaaagtatc cgccgatgaa
     8941 ctcgatcacg aactcgctcg cctacgcgct cgccaacatc ctgcacccgc gcatgatctg
     9001 gctgatgatc tggccgctgg tcatcgcgct cggcctgtgg ggcacgctcg cgatggtgtt
     9061 ctgggcgcag ctcgcgctgg cgctggccga gtggatgcag agcggcctcg cccatgcgcc
     9121 gctgatcggc agctgggact tcaccacggc gaccctgttc ctcgccaagg tgctgatcct
     9181 cgtcatgctg gtgccgctga tccagctcac cgcgcttctc atcctctcgg tgttcggcat
     9241 gccctcgatg gtcgagcacg tggcgagccg cagctttacg cggctggagc ggcgcaaggg
     9301 cggcagcctc gccggcagcg tgtggaactc cagcgtcggc ctcgccggcc tggtgggcct
     9361 cggcattgcc tcgatcccgc tctgggtgtt cccgccgctg tggccgctga tcccggtggc
     9421 gatcctcgcc tgggtgaacc agcgcgtgct ccggtacgac gcgctcgccg agcacgccgc
     9481 ggccgacgag atgcgccggc tgttctcggc gcgctggggc gggctgtacg tgatgggcct
     9541 cgtcctcgcg ctcctcgcct acatcccgat ccttggcttc tttgcgccgg tgctgctcgg
     9601 cgtgagcttc atccattacc tcctgggcgc gctcgaggag cagcggggcg cgccgatcga
     9661 gggccaggtc gtgtgaccgg cttcggcgcc ttcgtcttcg gcgacgagat cctcgtcggc
     9721 aagcgccagg acaagcactt tccctggctc atcggggcgc tggggaaacg cggcctgcgc
     9781 ctcgcctggt gcgagcacct cggcgacgag ccggcgcgcg tgacgcaggc gctcaagcgc
     9841 accttcgcga cccaggacgt cgtattttgc tttggcggca tcggcgcgac gcccgatgac
     9901 cacacgcgcc agtgcgccgc cgcggcgctc ggcgtcgagc tggccctgca tccggaggcc
     9961 gaagccgaga ttcgcggccg cttcggcgcg gaaaccaacc cgcagcgcct gaagatgggc
    10021 gagttcccgg ccggcgcgca gatcatcccc aaccccttca accgcatccc gggcttctcg
    10081 gtcggccacc attatttcgt gcccgggttc ccgcagatgg cctggccgat gatcgagtgg
    10141 gtgctggacg agaagtaccg gcagcttttc gactcgcagc gctgggccga gggctcgctc
    10201 atcgtctacg acctgccgga gtcggcggtc gcgccgctga tggaggagct gaaccgggac
    10261 ttcccgggcc tgaaggcctt cagcctgccc agcatgggcg ccgacggcac ccgccggcac
    10321 atcgagctcg gcgttcgggg cgcccaggac gagattgcgc cggcgatgga gcggttgcgc
    10381 cggggcgtgg ccgagctggg cggcgccctg gacgttccaa agcgctgatt tctcccgtat
    10441 aatccgcgcc ttttcgcggt tcacggggtt tccaaaatgg tggtgattcg actggcgcgc
    10501 ggcggcgcga acaagcgtcc cttcttcaac ctggtggtgg cggactcgcg ccgcgccgcc
    10561 aagggcaagt tcatcgagcg cgtcggcttc tacgacccga aggcgccgga gggccgcgag
    10621 gcgctgcgcg tgaacgtgga gcggctcaag cactggcagg gcaagggcgc gcagctctcg
    10681 accacggtga agcggctcgt gaagcagttt ggcaagaagg ccgcctgagc tgatcgcgat
    10741 gggccgcgtc gccggttcgt acggcgtgcg cggctgggtg aaggtggcgc cgcaggcggg
    10801 cgtgcaggaa agcctggcgg cggcgaagca gtggtggctc gatgaagcgc cgcggcgcgt
    10861 cgaggctgcg cgagtgcaca gcgcgacggt gatcgcgaag ctcgaggggg tcgaatcccg
    10921 cgagcaggca ctggcgctga agggccgggt cgtgtcggtg gcgcgcgcgg cgctgccgcg
    10981 ggcggaggcg gggcactact acctcgccga cctgctcggg ctgcaagtgg tgaacacgca
    11041 gggcgtggcg ctcggcgtgg tgcggcagtg gctgacgaac gggccgcagg acgtgatgga
    11101 gctggccggc gaccaggtgc ggctcgtgcc ctgggtcgcg gcggtggtga agaacgtgga
    11161 cctcgaggcg aaggtgatcg aggtggaatg ggaagcggac tggtgatccg cttcgacgtg
    11221 gtgacgctgt tcccggagat gttcgctgcc gtcacggcga gcggcatccc gagaaaggcg
    11281 ctggaagcag ggctgtggcg gttggcgacg tggaatccgc gcgatttcac caccgacaac
    11341 taccgcacgg tcgacgaccg gccctacggc ggcgggcccg gcatggtgat gctggccgag
    11401 ccgctggaaa aggcgctcga tgcggccaag gcggcgggcg ggggcgcggt gctgtacctc
    11461 tcgccgcagg ggcgcaggct cgatcacgcg aaagtgatgg agctggcggg cagaggcgcg
    11521 gtgacgctgc tgtgcggccg ctacgaaggc gtggatgagc ggctcatccg gcgccgggtg
    11581 gacgaggaat tgtcgctcgg ggacttcgtg ctctcgggcg gggagctcgc ggcgatggcg
    11641 gtgatcgacg ccgtggtgcg gcagttgccc ggggcgctgg gcgacgggca gtcggcggcc
    11701 gaagagtcgt tcgccgccgg gctgctggac tgcccgcagt acacgcggcc cgaggtgtac
    11761 gcgggcgaga aggtgccgga ggtgctgctc tccggtcacc atgagcagat ccggcgctgg
    11821 cggctgcagc aggcgctggg acggacctgg ctgcggcgcc ccgagctgat cgcggcgcgg
    11881 cagctgagcg gcgaggaaca caagctgctt gaggaattca ggcagcaggc gaaagagcgg
    11941 agcgaaagat gaacctgatt gagactatcg agaaagaaga gatcaagcgg ctggggaaga
    12001 cgattcccga tttctcgccc ggcgacacgg tcgtggtgaa cgtcaccgtg gtcgaaggcg
    12061 agagaaagcg cgcccaggcc ttcgagggcg tggtgatcgc gaagaagaac cgcggcatcg
    12121 gctcctcgtt caccgtgagg aagatcagct ccggcgaggg cgtggagcgc actttccaga
    12181 cctactcgcc gctcatcgcc tcgatcgagg tgaagcgcaa gggcgaggtg cggcgcgcca
    12241 agctctacta cctgcgccag cgctcgggca agtcggcgcg catccgcgag aaggtcggcg
    12301 gcgcgagcgc ggcgggcgag ggcggcgagg agtagccgca ggcggcagag ctggcggcag
    12361 cgcgccgcgt ctgcagtaca aaacggtctc ggcttttccg gcggggtgaa tcttcccgcc
    12421 ggaaaagccg agattctttt aaaagccaaa atcgcgcgct gaatcgctcc gcgccccttt
    12481 tcgtaccata atcccgccat gcgccgcgaa ctcaccgcca tcagcccggt cgccgacatc
    12541 gtcgccgacc tgcgcgccgg caagatggtc gtcctggtcg acgacgagga ccgcgagacc
    12601 gagggcgacc tcgtcttcgc cgccgagttc gtcacgccgg agaaggtcaa cttcctcgcg
    12661 cgccacggca ggggcctcat ctgcatgccg atcaccgagg cgcacgcgct gcgcctgggc
    12721 ctgcggccga tggtggagca gaaccgctcg cgccacggca ccaacttcac cgtctcgatc
    12781 gaggccgccg agggcatcgc caccggcatc tcggcagcc
//
LOCUS       DBNHKE010000116        13378 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_812976, whole genome shotgun sequence.
ACCESSION   DBNHKE010000116 DBNHKE010000000
VERSION     DBNHKE010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_812976"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="AC16O9_07270"
     CDS             <1..1577
                     /locus_tag="AC16O9_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR6516453.1"
                     /translation="ILKAPVELLYNGGIGTYVKASPESHAQVGDRANDALRVDGRELR
                     CKVVAEGGNLGFTQLGRIEYALGGGRINTDAIDNSAGVDCSDHEVNIKILLDAAVREG
                     ELTGKQRDRLLAEMTDEVAQLVLRDNVFQTQSLSVAGRVAPALLEQQARFIRALERAG
                     RLNRAIEYLPADEEIAARRSARRGLTAPERAVLLAYSKMQTYEELCASDIPDDPTIAT
                     ALERYFPQPLRKRYARHIQAHPLRREIIATHVTNSMANRVGSTFVHRMREESGASTAE
                     VVRAYMVTREAYGLVAFWRQVEALDLKVADEVQAAMLLDAGRLVVRATLWLLRNRKHL
                     ADVAASVAHFRPGIEALGALLPEAFAESERQGFEQASRRYLEAAVPPALAAQVARFDA
                     LPAALDLVEVGEALGRDVRTVAQAYFALGGRLEFPWLRSCISGLPAESHWQTLAKAAL
                     RDDLAGMQRQLAADVLRGADGADTTRALAAWEETNRALLERFRQVHADLRTHKTLDLA
                     MASVAMRELRNIAARG"
     gene            complement(1590..3248)
                     /gene="ettA"
                     /locus_tag="AC16O9_07275"
     CDS             complement(1590..3248)
                     /gene="ettA"
                     /locus_tag="AC16O9_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6516454.1"
                     /translation="MAQYVYTMRRVQKIVPPKREILKNISLNFFPGAKIGVLGLNGSG
                     KSSLLKIMAGEDPNYEGEVVRMPNIKVGFLPQEPALEPEKTVRETVEESQGDLAAAKK
                     RLDEVYAAYAEPDADFDKLAAEQARLEALVNAAGSGQELEIAADALRLPPWEQKVGVL
                     SGGEKRRVALCRLLLSNPDLLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDRYF
                     LDNAAGWILELDRGRGIPWEGNYSSWLEQKEKRLELEQSQEAARIKSMKAELEWVRQN
                     PKGRQAKSKARLARFEELSQYEHQQRNETQEIFIPVAERLGNEVIEFKNVSKGYGERL
                     LIDKLSFKVPPGAIVGIIGPNGAGKSTLFRMLIGKDKPDSGKIMLGKTVKLAHVDQSR
                     DALQGDKTVFEDISGGQDNITVGKFTMPSRSYLARFNFRGPDQQKQVGQLSGGERGRL
                     HLAKMLMSGANVLLLDEPSNDLDVETLRALEEALLEFAGSVLVISHDRWFLDRIATHI
                     LAAEDDSQWVFFDGNYQEYEADKKKRLGEEAARPHRIKYKPLKK"
     gene            complement(3248..5524)
                     /locus_tag="AC16O9_07280"
     CDS             complement(3248..5524)
                     /locus_tag="AC16O9_07280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6516455.1"
                     /translation="MNDPILSASQWIGKPLRRLEDQRLLTGAGRYTDDVRPEGALHVA
                     FLRSPHAHARIAKMDVSAARGAPGVAAVYLGADVVADGVGPIPFTHLHKRQDGSPIVA
                     PPRMPITADEARFVGDAVAMVVAETRHQARDAAEMIEVEWEPLEAIPDLPGAARAHND
                     VAYYHAGDKAAREAAFAAAAHVVRIQVVNQRVVANPLEPRALAAQYHADTGRYTLWCP
                     TQNVHNTRAQLAGALGVKEEALRIVSTDVGGGFGARGYAYPEHAALLWAAKKLGRPLR
                     WLADRSENFLAEVHGRDNLTVAELALDAGHRFTALRIRTLANVGAYISSFGAAVPAMS
                     GVRAPTGVYAIPVLDHEVKMMFTNTTPVDAYRGAGRPEMGYLLERLVERAAREVGADP
                     IALRLKNLIPATRMPYANPAGYTYDCGDFEKVLRAALGAADWDGFAGRKAGAEARGLR
                     YGRGLACYVEITGSARLSETVRMIAAADGTLEVDSGTQAIGQGLWTSYAQIVADKLGI
                     DPQAIRVVQGDSDLLKAGGGAGGSRGLQVGGSAALAGAIALIEAARPLAAQALEAAVA
                     DLEFGDGRFRIAGTDRGIGLFELAGKQPAQRIVCEHTETAKGQTWPNGCQIAEVEVDP
                     ETGAVRVARHSAVDDIGRVINPLTAESQMHGGVAQGLGQALFEQSVYDETGQLLTGSF
                     MDYAMPRAADMPAAMQASFEQSVPTAQNLLGAKGAGEAGCHGAVPAIVNAVIDALGVK
                     EIDMPLTPEKVWRALQER"
     gene            5569..6444
                     /locus_tag="AC16O9_07285"
     CDS             5569..6444
                     /locus_tag="AC16O9_07285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516456.1"
                     /translation="MTTAEVLGFIGTGVMGEPMCRNLAAKSGARVIAWDPQRAPLERL
                     RTHSVTLAASPGELAAQAAIVFACLPGGAQLESLCETALLPAARAGLTIVDLGTSGVG
                     RTRARAQRFSARGADYADAPIARTRAAAEAGTLAISVGATPEVFARIRPLLACIGTDI
                     THCGGVGSGQVVKILNNMVLFQTVNALAEALALGRRAGVEPALLFEALAKGSADSFAL
                     RNHGMKAMLPGEYPERAFSVEYASKDNGYALELGRETGLRLRGAETVAALFDEARAAG
                     YGGAYYPVIAKVVDR"
     gene            complement(6452..7174)
                     /locus_tag="AC16O9_07290"
     CDS             complement(6452..7174)
                     /locus_tag="AC16O9_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR6516457.1"
                     /translation="MNTKLAAGLLPLCLAVALDAQAAHPLITEDTGTQGAGRYQLEVF
                     AEELEERGTRRDIEVWTGVLSYGVGETADVQVGIPVYRDGPDGVGDASLGLKWRFLER
                     NAWSLALKPGITVPTGDERDGRGTGKVTWGSLIIVSYAPGAIAVHAHAGFWRNENKLG
                     ERESLRQLAAAATYRIGDVRFVGEFARETNPAPGGRTVRYSTIGAIWSMTRDVDLDIG
                     WRNGNGSAPIDEALLIGATVRW"
     gene            7204..7500
                     /locus_tag="AC16O9_07295"
     CDS             7204..7500
                     /locus_tag="AC16O9_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6516458.1"
                     /translation="MPYAILTTDKPNSAELRARVRDTHIEYLTANVHKLLAAGALIND
                     DGSGGHGGILIVDTDDRKDAEAFIAADPFAKAGLFEKVTVTRWRKAYFNKQKLI"
     gene            complement(7501..8610)
                     /locus_tag="AC16O9_07300"
     CDS             complement(7501..8610)
                     /locus_tag="AC16O9_07300"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6516459.1"
                     /translation="MKTVICIVAALATLPAVAQVHTSAEHSFRVVELVRGLEQPWALA
                     FLPDGRMLVTEKAGRLRIVKDGRLDPQPVAGLPEVTVVGQGGLMDVALHPRFKENSLV
                     YISFTARGIDGVGTEVARGRLADHRLEDAQIIFRQNPKGSTGRHFGSRLVFDRQGYLY
                     ITLGDRGEMERAQRPGDHAGSVIRLHDDGRVPEDNPFVGKPGWKPEKYTLGNRNQQGA
                     ALHPQTGVLWTQEHGPQGGDEVNVIRAGANYGWPVITYGVQYVVGTQIGEGTHKPGME
                     QPVWKWVPSIAPSGMAFYTGDKFPKWRGDLFIGALKAQLLVRLELDGEKVVREERLLQ
                     GALGRIRDVRSGPDGFLYLLTDESRAVLARLEPAP"
     gene            8631..9335
                     /locus_tag="AC16O9_07305"
     CDS             8631..9335
                     /locus_tag="AC16O9_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR6516460.1"
                     /translation="MAIARFRFHGGLAAFLNKEHRDRSFVHACARAATLKNAIEALGV
                     PHTEAGRVIVNGAPATLQRAVRDGDLIEVFATTTQEGIDLIFLADAHLGGLARFLRML
                     GFDTLHDNRFADAEIRRLAEAERRIVLSRDRELLKCREIAHGCYVRALKAEAQLREVA
                     ARYGLAARARPFTLCLRCNLPLLPATKAQVAALAPPQVLEHQTQFTCCAGCGRVYWPG
                     SHYARMRAALGDLLGG"
     gene            9343..10395
                     /locus_tag="AC16O9_07310"
     CDS             9343..10395
                     /locus_tag="AC16O9_07310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6516461.1"
                     /translation="MPTAAVVGAQGVIGRYIVEKLASLPDWSVVGLSRRKGADAPRVK
                     HIPVDLLDPRDARAKLASLKDVTHVFFAAFQPGSGPAADYAKNIAANLELLVNSVSPI
                     DQASSKLERVVLVTGTKYYGSHLGPFKTPAKEDDPRHAGPNYYFDQIDWLAEYKKGKR
                     WSWTELRPQTLCGFAPGTAMSIVSVIAVYAAMRKELGLPLAFPGTAWSSLYQVTESAH
                     IAEAAHWAATEPRCANQAYNITNGDYFRWQHVWPAIAKVFDMPVAEPQPHALVDFMAD
                     KAPLWDAMVKKHGLKPYSFKDVVAWPFGDYVFNCDWDVMTSTTKCRQHGFHAVVDSEE
                     MFGRLLRRFRAERIVP"
     gene            complement(10403..11575)
                     /locus_tag="AC16O9_07315"
     CDS             complement(10403..11575)
                     /locus_tag="AC16O9_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6516462.1"
                     /translation="MSAAARPLEGIRVLEMGQLLAGPFAGCVLGYFGAEVIKVEPPGG
                     DPIRGWRVVRDGTSLWWASLARNKKSVTLDLGTPRGREIAKQLAAKCDVLIENFRPGT
                     LEKWGMSPAELKQLNPELIVARVSGYGQTGPYAAQPGFASVCEGFAGFRHVNGFPDRP
                     PVRPNLSMGDTLAGLHAVLGILLALTARLRGKAKAGQVVDVAIYESVFNLLESVVPEY
                     DGAGVIREPSGSTLTGIVPTNTYLCADGKYVIIGGNGDSIYQRLMRAIGRPEMAEDAR
                     FATNAKRVENEKEIDAAIGAWTKARSSAEVLEALRQAGVPNGPIYNVADMMADPHFNA
                     RGMFEPVEVNGKPLKIPAIPPLLGETPGRTDAPGPALGAHTEEVLGKLLGLKPPFE"
     gene            complement(11572..12333)
                     /locus_tag="AC16O9_07320"
     CDS             complement(11572..12333)
                     /locus_tag="AC16O9_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR6516463.1"
                     /translation="MLNFGLGFLALIILLIIASIRILREYERGVVFLLGRFQRVMGPG
                     LIIVIPGIQQMVRVDLRLIVLDVPSQDVISRDNVSVKMNAVVYFRVVDPQLAIIQVAN
                     FLDATSQLAQTTLRSVLGKHELDEMLAERDKLNADIQTILDQQTDAWGIKVANVEIKH
                     VDLNESMVRAIAQQAEAERARRAKIIHAEGEMQASEKLLEAANMLSQRAEAMQLRYLQ
                     TLTQIASDKSHTIVFPLPMDVLGPILEAAKKLKGA"
     gene            complement(12350..>13378)
                     /locus_tag="AC16O9_07325"
     CDS             complement(12350..>13378)
                     /locus_tag="AC16O9_07325"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6516464.1"
                     /translation="AAHIAAMAPATNLGAASPVAIGAPSGGGDKPDPSGKDAKKGAAP
                     AGDTMMRKATNDAAAYIRSLAELRGRNAEWGERAVREAVSLSAAEAKKIKVIEIVADS
                     VPALLQAVDGRTVEAGGEKRKLATAGAQVTEIEADWRTNVLRVITNPSVAYLMILLGI
                     YALIFEFSNPGLILPGVVGAICILIAMYAFQLLPVNYAGLALLLLGIAFMVAEVWVAA
                     HGALAVGGLVAFVIGSIMLLDTDIPQFEIPYTLIAGVTVGSAAFVFLVVGMMVKNRAR
                     PVVAGREYLLGASGEALEDISGEGWARVQGERWRVRTKEPLRAGERLRITAVHGLVLD
                     ATRASADE"
BASE COUNT         1934 a         4787 c         4581 g         2076 t
ORIGIN      
        1 cgatcctcaa ggcgccggtc gagctgctct acaacggcgg catcggcacc tacgtcaagg
       61 caagcccgga atcgcacgcg caggtcggcg accgcgccaa cgacgcgctg cgcgtcgacg
      121 gccgcgagct gcgctgcaag gtggtggccg agggcggcaa tctcggcttt acgcagctcg
      181 gacgcatcga gtacgcgctc ggcggcgggc gcatcaacac cgacgccatc gacaactcgg
      241 cgggcgtgga ctgctcggat cacgaggtga acatcaagat cctgctcgac gcggcggtgc
      301 gcgaaggcga gctcaccggc aagcagcgcg accgcctgct cgccgagatg accgacgagg
      361 tcgcgcagct cgtgctgcgc gacaacgtgt tccagacgca gagcctgtcg gtggcggggc
      421 gcgtggcgcc ggcgctcctc gagcagcagg cgcgcttcat ccgggccctc gagcgcgccg
      481 ggcgcctgaa ccgcgccatc gagtacctgc ccgccgacga ggagatcgcg gcgcgccggt
      541 cggcgcgccg cgggctgacc gcgcccgagc gcgcagtgct gctcgcctac tccaagatgc
      601 agacttacga agagctatgc gcctcggaca ttccggacga tcccactatc gccacggcgc
      661 tcgagcgcta cttcccgcag ccgctgcgca agcgctacgc tcgccacatc caggcgcatc
      721 cgctgcgccg cgagatcatc gccacgcacg tcaccaacag catggccaac cgcgtcggca
      781 gcaccttcgt gcaccgcatg cgcgaggagt ccggggcaag tacggcggaa gtcgtgcgcg
      841 cgtacatggt gacgcgcgaa gcctacggcc tggtggcgtt ctggcggcag gtcgaggcgc
      901 tcgatctcaa ggtggcggac gaggtgcagg ccgcgatgct tctcgacgcg ggccggctgg
      961 tggtgcgtgc gacgctgtgg ctgctgcgca accgcaagca cctcgccgac gttgccgcgt
     1021 cggtcgcgca cttccgtccg ggaatcgagg ccctcggcgc gctgctgccg gaggccttcg
     1081 ccgagagcga gcgccagggc ttcgagcagg cgagccggcg ttacctcgag gccgccgtac
     1141 cgcccgcgct cgccgcgcag gtagcgcgct ttgacgcgtt gccggcggcg ctcgacctgg
     1201 tggaggtcgg cgaggccctc ggtcgcgatg tgcgcaccgt ggcgcaggcc tacttcgcgc
     1261 ttggcggccg gctcgagttt ccctggctgc gctcgtgcat ctccggcctg ccggctgaga
     1321 gccactggca gacccttgcc aaggcggcgc tgcgcgacga cctcgccggc atgcagcgcc
     1381 agctggccgc cgacgtgctg cgcggggcgg acggcgccga cacaacgcgc gccctggccg
     1441 cgtgggagga gaccaaccgc gcattgctcg agcgcttccg ccaggtgcac gccgacctgc
     1501 gcacccacaa gacgctcgac ctggcgatgg cttcggtggc gatgcgcgag ctgcgcaaca
     1561 tcgcggcgcg cggctgaggc ggcggccgct tacttcttca gcggcttgta cttgatgcgg
     1621 tgcggccgcg cggcttcctc gcccaggcgc ttcttcttgt cggcctcgta ttcctggtag
     1681 ttgccgtcga agaacaccca ctgcgaatcg tcttcggcgg ccaggatgtg cgtggcgatg
     1741 cggtcgagga accagcggtc gtgcgagatc accagcacgc ttccggcaaa ctcgagcagc
     1801 gcctcttcca gcgcgcgcag cgtctcgacg tcgaggtcgt tggagggctc gtccagcagc
     1861 agcacgttgg ccccggacat cagcatcttg gccaggtgca gccgcccgcg ctcgccgccc
     1921 gagagctgtc ccacctgctt ctgctggtcg ggcccgcgga agttgaagcg cgcgaggtag
     1981 gaccgggagg gcatggtgaa cttgcccacc gtgatgttgt cctgtccgcc ggagatgtcc
     2041 tcgaacaccg tcttgtcgcc ctgcagcgcg tcgcgcgact ggtcgacatg cgcgagcttc
     2101 accgtcttgc caagcatgat cttcccggaa tccggcttgt ccttgccgat cagcatccgg
     2161 aacagcgtgg acttgccggc gccgttcggg ccgatgatgc ccacgatggc gcccggcggc
     2221 accttgaagg aaagcttgtc gatgagcagc cgctcgccat agcccttgga gacgttcttg
     2281 aactcgatca cctcgttgcc gaggcgctcg gcgacgggga tgaagatctc ctgcgtctcg
     2341 tttctctgct ggtgctcgta ctgcgacagc tcctcgaaac gcgccaggcg cgccttggac
     2401 ttggcctgcc gccccttggg gttctggcgc acccattcga gctcggcctt catggacttg
     2461 atgcgcgccg cctcctggct ctgctcgagc tcgaggcgct tttccttctg ctccagccag
     2521 gacgagtagt tgccctccca gggaatgccg cgcccgcgat cgagctccag gatccagccc
     2581 gcggcgttgt cgaggaagta gcggtcgtgc gtcaccgcca ccaccgtgcc cgggaagcgg
     2641 accaggaact gctcgagcca ctccacgctc tcggcgtcga ggtggttggt cggctcgtcg
     2701 agcagcagca ggtcggggtt cgatagcagc agccggcaca acgccacgcg ccgcttctcg
     2761 ccgccggaga gcaccccgac cttctgctcc cacggcggca gccggagcgc gtcggccgcg
     2821 atctcgagct cctggccgct gcccgcggca ttcaccagcg cttccagtcg cgcctgctcc
     2881 gcggcaagct tgtcgaagtc ggcgtcgggc tcggcgtagg cggcgtagac ctcgtcgagg
     2941 cgcttctttg ccgcggcgag gtcgccctgg ctctcctcga ccgtctcgcg cacggtcttc
     3001 tccggctcga gcgccggctc ctgcggcagg aagcccacct tgatgttggg catgcgcacc
     3061 acttcgccct cgtagttcgg gtcctcgccc gccatgatct tgagcaggct cgacttgccc
     3121 gagccgttca ggcccaggac gccgatcttg gcgcccggaa agaagttgag cgagatgttc
     3181 ttcaggatct cgcgcttcgg gggcacgatc ttctgcaccc ggcgcatggt gtagacgtac
     3241 tgagccatca gcgctcctgc agcgcgcgcc acaccttttc gggcgtgagc ggcatgtcga
     3301 tctccttcac gccgagcgcg tcgatcaccg cgttgacgat cgcgggcacg gcgccgtggc
     3361 agcccgcttc gcccgcgccc ttggcgccga gcaggttctg cgccgtgggc acggactgct
     3421 cgaagctcgc ctgcatcgct gccggcatgt cggcggcgcg cggcatggcg tagtccatga
     3481 acgagccggt gagcagctgc ccggtctcgt cgtacaccga ctgctcgaac agcgcctggc
     3541 cgagtccctg tgccaccccg ccgtgcatct gcgattcggc agtcagagga ttgatgacgc
     3601 gcccgatgtc gtccaccgcg ctgtggcgcg cgacgcgcac cgcgccggtc tcggggtcga
     3661 cctcgacctc ggcaatctgg cagccgttcg gccaggtctg ccccttggcc gtctcggtgt
     3721 gctcgcagac gatgcgctgc gccggctgct tgcccgcgag ctcgaacagg ccgatgccgc
     3781 ggtcggtgcc ggcaatgcga aagcgcccgt cgccaaattc gagatcggcc acggcggctt
     3841 ccagcgcctg cgccgcgagc gggcgcgccg cctcgatcag cgcgatggcg ccggccagcg
     3901 ccgcgctgcc gccgacctgc agcccgcgcg agccgccggc gcccccgccc gccttgagca
     3961 ggtcgctgtc gccctgcacg acgcggatcg cctgcgggtc gatgccgagc ttgtccgcca
     4021 cgatctgggc ataggacgtc cacagcccct ggccgatcgc ctgggtgccc gaatccactt
     4081 ccagcgtgcc gtcggccgcc gcgatcatgc gcaccgtctc gctcaggcgc gcgctgcccg
     4141 tgatctcgac gtagcacgcc agcccgcgcc cgtagcgcag cccgcgcgcc tcggcgcccg
     4201 ccttgcgccc cgcaaagccg tcccagtcgg cggccccgag cgccgcgcgc agcaccttct
     4261 cgaagtcgcc gcagtcgtag gtgtagccgg ccgggttcgc atagggcatg cgcgtggccg
     4321 ggatcaggtt cttcagccgc agcgcgatcg ggtcggcgcc cacctcgcgc gccgcgcgct
     4381 cgaccagccg ctcgaggaga tagcccatct ccggccgccc cgcgccacgg taggcgtcca
     4441 ccggcgtggt gttggtgaac atcatcttca cttcgtggtc gagcaccggg atcgcgtaga
     4501 cgccggtggg cgcgcgcacg cccgacatcg cgggcaccgc ggcgccgaag ctcgagatgt
     4561 aggcgccgac gttggcgagc gtgcggatgc gcagcgcggt aaagcgatgc ccggcgtcca
     4621 gcgcgagctc ggcgaccgtc aggttgtcgc gcccgtgcac ctcggcgagg aagttctccg
     4681 agcggtcggc gagccagcgc aggggccgcc cgagcttctt cgccgcccac agcagcgccg
     4741 cgtgctccgg ataggcgtag ccgcgcgcgc cgaagccgcc gcccacgtcg gtgctgacga
     4801 tgcgcagcgc ctcctccttg acgcccagag cgcccgcgag ctgcgcgcgc gtgttgtgca
     4861 cattctgcgt ggggcaccac agcgtgtagc ggccggtgtc cgcgtggtat tgcgccgcga
     4921 gcgcgcgcgg ctcgagcgga ttcgccacca cgcgctggtt caccacctgg atgcgcacca
     4981 cgtgcgccgc cgccgcgaac gccgcctcgc gcgccgcctt gtcgcccgcg tgatagtagg
     5041 cgacgtcgtt atgcgcgcgc gcggcgcccg gcaggtcggg aatcgcctcc agcggctccc
     5101 actcgacctc gatcatctcc gcggcgtcgc gcgcctggtg gcgcgtttcg gcgaccacca
     5161 tggccacggc gtcgccgacg aagcgcgcct cgtcggccgt gatcggcatg cgcggcggcg
     5221 cgacgatggg cgagccgtcc tggcgcttgt gcaggtgcgt gaaggggatc ggcccaacgc
     5281 cgtcggcaac gacgtcggcg ccgaggtaga cggcggcgac gcccggtgcg ccgcgcgcgg
     5341 cagagacatc catcttcgcg atgcgcgcgt gcgcgtgcgg cgagcgcagg aaggcgacgt
     5401 gcagcgcccc ttcggggcgc acgtcgtcgg tgtagcggcc cgccccggtg agcaggcgct
     5461 ggtcctcgag gcgccgcagg ggcttgccga tccactggct ggcggaaagg atggggtcgt
     5521 tcattggggg caaagcgcgc aattctatac tgcgtgcctg gaggctccat gaccacagca
     5581 gaggttctcg gcttcatcgg cacgggcgtg atgggcgagc ccatgtgccg caatctggcc
     5641 gcgaagagcg gcgcccgggt gatcgcctgg gatccgcagc gcgcgccgct cgagcgcctg
     5701 cgcacgcaca gcgtgacgct cgccgccagc ccgggcgagc tcgccgcgca ggcagcgatc
     5761 gtcttcgcct gcctgccggg cggcgcgcag ctcgaatcgc tctgcgaaac agcgctcctg
     5821 cccgcggcac gcgccggact gacgatcgtg gatctgggca cctccggcgt cggccgtacg
     5881 cgcgcgcggg cgcagcgctt cagcgcgcgc ggcgccgact acgcggacgc gcccatcgcg
     5941 cgcacgcgcg cggccgccga ggccggtacg ctcgccatca gcgtcggcgc gacacccgag
     6001 gtgttcgcgc gcatccggcc gctgctcgcc tgcatcggca ccgacatcac gcactgcggc
     6061 ggcgtcggca gcgggcaggt ggtgaagatc ctcaacaaca tggtgctgtt ccagaccgtg
     6121 aacgcgcttg ccgaggcgct ggcgctcggg cggcgcgccg gcgtggagcc ggcgctgctg
     6181 ttcgaggcgc tcgccaaggg ctcggccgac agcttcgcgc tgcgcaacca cggcatgaag
     6241 gcgatgctgc cgggcgagta cccggagcgc gccttttcgg tggagtacgc gtccaaggac
     6301 aacggctatg ccctggagct cggtcgcgaa accgggctgc gcctgcgtgg cgcggaaacc
     6361 gtcgccgcgt tgttcgacga ggcgcgcgcc gccggctatg gcggcgccta ctacccggtg
     6421 atcgcgaaag tcgtggaccg ctgaagcgcg cctaccagcg taccgtggcc ccgatgagca
     6481 gcgcctcgtc gatcggcgcg ctgccattgc cgttgcgcca gccgatgtcg aggtccacgt
     6541 cgcgcgtcat cgaccagatg gcgccgatcg tggagtagcg caccgtgcgc ccgccgggcg
     6601 ccggattggt ctcgcgcgcg aactcgccca cgaagcgcac gtcgccgatg cggtaggtgg
     6661 cagccgcggc cagctggcgc agcgattcgc gctcgccgag cttgttctcg ttgcgccaga
     6721 atccggcgtg cgcgtgcacg gcgatggcgc ccggcgcata cgacacgatg atcagcgacc
     6781 cccaggtgac cttgccggtg ccgcgcccgt cgcgctcgtc gccggtgggc acggtgatgc
     6841 cgggcttgag cgccaggctc caggcgttgc gctcgaggaa gcgccacttg aggccgagcg
     6901 aggcgtcgcc gacgccgtcg ggcccgtcgc ggtacaccgg aatgccgacc tggacgtcgg
     6961 cggtctcgcc gacgccgtag ctgagcacgc cggtccagac ctcgatgtcg cggcgcgtgc
     7021 cgcgctcctc cagctcttcg gcaaacactt cgagctggta gcggccggcg ccctgcgtgc
     7081 cggtgtcctc ggtgatcagc ggatgcgcgg cctgcgcgtc gagcgcgacc gcaaggcaaa
     7141 gcggaagaag tcctgcggca agtttagtat tcatgttccc gactgtcgtg gaaaggagac
     7201 gccgtgccct acgccatact gacgactgac aagccgaaca gcgcagagtt gcgcgccagg
     7261 gtgcgcgaca cgcacattga gtatctcacg gccaacgtgc acaagctgct cgcagccggc
     7321 gcgctgatca acgacgacgg cagcggcggc cacggcggca tcctcatcgt cgacaccgac
     7381 gaccgcaagg acgccgaggc cttcatcgcg gcagacccct tcgcgaaggc cgggctgttc
     7441 gagaaggtga cggtaacgcg ctggcgcaag gcctacttca acaagcagaa gctgatctag
     7501 ctagggcgcg ggctccagcc gcgcgagcac ggcgcgcgac tcgtcggtga gcaggtagag
     7561 gaagccgtcg gggccgctgc gcacgtcgcg gatgcgcccg agcgcgccct ggagcaggcg
     7621 ctcctcgcgc accaccttct cgccgtcgag ctcgagccgc accaggagct gcgccttgag
     7681 cgcgccgatg aacaggtcgc cgcgccactt cggaaatttg tcgcccgtgt agaaggccat
     7741 gcccgagggc gcgatcgacg gcacccattt ccacaccggc tgctccatgc cgggcttgtg
     7801 cgtgccctcg ccgatctgcg tgccgacgac gtactgcacg ccgtaggtga tcaccggcca
     7861 gccgtagttg gcgccggcgc ggatcacgtt cacctcgtcg ccgccctgcg ggccgtgctc
     7921 ctgcgtccag aggacgccgg tctgcggatg gagcgccgcg ccttgctgat tgcggttgcc
     7981 gagcgtgtac ttctccggct tccagcccgg cttgccgacg aacgggttgt cctcgggcac
     8041 gcggccgtcg tcgtgcaggc ggatcaccga gccggcgtgg tcgcccgggc gctgcgcgcg
     8101 ctccatctcg ccgcggtcgc cgagcgtgat gtacaggtag ccctgccggt cgaacacgag
     8161 gcgcgagccg aagtggcgcc ccgtgctgcc cttggggttc tggcggaaga tgatctgcgc
     8221 gtcctcgagc cggtggtccg cgaggcgccc gcgcgcgact tccgtgccga cgccgtcgat
     8281 gccgcgcgcg gtaaaagaga tgtaaaccag gctgttctcc ttgaagcgcg gatgcagcgc
     8341 cacgtccatc aggccgccct gcccgaccac cgtgacctcg ggcagtccgg cgacgggctg
     8401 cgggtcgagg cggccgtcct tgacgatgcg cagccgcccg gccttctccg tgaccagcat
     8461 gcggccgtcg ggcaggaagg cgagcgccca gggctgctcc aggccgcgca ccagctcgac
     8521 gacgcgaaag ctgtgctcgg cggaagtgtg cacctgcgcg acggcgggca gcgtggcgag
     8581 cgcggccacg atacaaatga cggttttcat gtgcctagca taatgccagc gtggcgatcg
     8641 cgcgctttcg cttccacggc gggctggcgg ccttcctgaa caaggaacac cgcgaccgct
     8701 cgttcgtgca cgcctgcgcg cgcgccgcga ccctcaagaa cgccatcgag gcgctcggcg
     8761 tgccgcacac cgaggccggg cgcgtcatcg tcaacggcgc cccggccacg ctgcagcgcg
     8821 cggtgcgcga cggcgatctg atcgaagtct tcgccacaac gacgcaagag ggaatagacc
     8881 tcatcttcct ggccgacgcg cacctcgggg gcctggcgcg cttcctgcgc atgctgggct
     8941 tcgacacgct gcacgacaat cgcttcgccg acgccgagat ccggcgcctg gccgaggccg
     9001 agcggcgcat cgtgctcagt cgcgaccgcg agctcctcaa gtgccgcgag atcgcgcatg
     9061 gctgctacgt gcgcgccctc aaggcagagg cgcaactgcg cgaagtggcg gcgcgctacg
     9121 gccttgcggc gcgcgcccgc ccgttcacgc tttgcctgcg ctgcaatctt ccgctgctgc
     9181 cggccaccaa ggcgcaggtt gccgcgctcg ccccgccgca ggtcctcgag caccagacgc
     9241 agttcacttg ctgcgccggc tgcggccgcg tgtactggcc tggttcgcat tacgcgcgca
     9301 tgcgcgccgc gctcggcgac ctgctcgggg gataatcccc tcatgccaac cgccgccgtc
     9361 gtcggcgccc agggcgtcat cgggcgctac atcgtcgaga agctcgcttc cctgcccgac
     9421 tggagtgtgg tcgggctttc gcgccgcaaa ggcgccgatg cgccgcgcgt caagcacatc
     9481 ccggtggacc tgctcgatcc gcgggacgcc agggcgaagc tcgccagcct caaggacgtg
     9541 acgcacgtct tcttcgccgc gttccagccc ggcagcggac ccgccgcgga ctacgccaag
     9601 aacatcgccg ccaacctcga gctgctggtg aactcggtct cgccgatcga ccaggcgtcc
     9661 tcgaagctcg agcgcgtcgt gctggtgacc ggcaccaagt actacggctc gcacctcggt
     9721 ccgttcaaga cgccggcgaa agaggacgac ccgcggcacg cgggccccaa ctactacttc
     9781 gaccagatcg actggctcgc ggagtacaag aagggcaagc gctggagctg gaccgagctg
     9841 cggccgcaga cgctgtgcgg cttcgcgccg ggcacggcga tgagcatcgt gtcggtgatc
     9901 gcggtgtacg ccgcgatgcg caaggagctc gggctgccgc tcgcctttcc cggcacggcc
     9961 tggtcctcgc tctaccaggt taccgagtcg gcgcacatcg ccgaagcggc gcactgggca
    10021 gcgaccgagc cgcgctgcgc caaccaggcc tacaacatca ccaatggcga ctacttccgc
    10081 tggcagcacg tgtggcccgc gatcgccaag gtgttcgaca tgcccgtcgc ggagccgcag
    10141 ccgcacgcgc tcgtggattt catggccgac aaggccccgc tgtgggacgc gatggtgaag
    10201 aagcacggcc tcaagcccta cagcttcaag gacgtcgtcg cctggccctt cggcgactac
    10261 gtcttcaatt gcgactggga cgtgatgacc agcaccacca agtgccggca gcacggcttt
    10321 cacgccgtgg tggacagcga ggaaatgttc gggcggttgc tgcgccgctt tcgcgccgag
    10381 cgaatcgtcc cgtaggtccg cgctattcga acggcggctt cagcccgagc agctttccca
    10441 gcacttcctc ggtatgcgcg ccgagcgccg gccccggcgc atcggtgcgc cccggcgtct
    10501 cgccgagcaa aggcggaatg gcggggatct tgagcggctt gccgttcacc tcgaccggct
    10561 cgaacatgcc gcgcgcattg aagtgcgggt cggccatcat gtcggcgacg ttgtagatcg
    10621 ggccgttcgg cacgcccgcc tggcgcaggg cctccagcac ctctgccgag ctgcgagcct
    10681 tggtccaggc gccgattgcc gcgtcgatct ccttctcgtt ctccacgcgc ttcgcgttgg
    10741 tcgcaaagcg cgcgtcctcg gccatctccg gccggccgat ggcgcgcatc aggcgctgat
    10801 agatcgagtc gccgttgccg ccgatgatca cgtacttgcc atccgcgcag agataggtgt
    10861 tggtcggcac gatgccggtg agcgtcgagc ccgaaggctc gcggatcacg cccgcgccgt
    10921 cgtactccgg caccaccgac tcgagcaggt tgaataccga ctcgtagatg gccacgtcga
    10981 cgacctgtcc ggccttcgcc ttgccgcgca gccgcgcggt gagcgcaagc aggatgccga
    11041 gcaccgcgtg caggccggcg agcgtgtcgc ccatggaaag gttcggccgc accggcggcc
    11101 ggtcgggaaa gccgttcacg tggcgaaagc ccgcgaagcc ctcgcacacc gaggcaaacc
    11161 cgggctgcgc ggcgtagggc ccggtctggc cgtagcccga gacgcgcgcg acgatcaatt
    11221 cgggattgag ctgcttcagc tccgcgggcg acatgcccca cttctcgagc gtgcccgggc
    11281 ggaagttctc gatcagcacg tcgcacttcg ccgcgagctg cttggcgatc tcgcgcccgc
    11341 gcggcgtgcc caggtcgagc gtgacggatt tcttgttgcg cgcgaggctc gcccaccaca
    11401 gcgacgtgcc gtcgcgcacc acgcgccagc cgcggatcgg gtcgcccccg ggcggctcca
    11461 ccttgatgac ctcggcgccg aaatagccca gcacgcagcc ggcgaagggg ccggccagca
    11521 actgccccat ctcgaggacg cggatgccct cgagcggccg tgccgccgcg ctcatgcgcc
    11581 tttcagcttc ttcgccgcct ccaggatcgg cccgaggacg tccatcggca gcgggaacac
    11641 gatggtgtgc gacttgtcgc tcgcgatctg cgtgagcgtc tgcaggtacc gcagctgcat
    11701 cgcctcggcg cgctgcgaca gcatgttggc ggcctcgagc agcttctccg aagcctgcat
    11761 ttcgccttcg gcgtggatga tcttggcgcg tcgcgcgcgc tcggcttccg cctgctgcgc
    11821 gatggcgcgc accatggatt cgttgaggtc gacgtgcttg atctcgacgt tcgccacctt
    11881 gatgccccag gcgtcggtct gctggtcgag gatggtctgg atgtcggcgt tcagcttgtc
    11941 gcgctcggcg agcatctcgt cgagctcgtg cttgccgagc acggagcgca gcgtggtctg
    12001 cgccagctgg ctggtggcgt ccaggaagtt cgccacctgg atgatggcga gctgcgggtc
    12061 gacgacgcgg aagtacacca ccgcgttcat cttcaccgag acgttgtcgc gagaaatcac
    12121 gtcctggctc ggcacgtcga gcacgatgag gcgcaggtcg acgcgcacca tctgctggat
    12181 gccggggatg acgatgatca gcccggggcc catgacgcgc tggaagcgcc cgagcaggaa
    12241 caccacgcca cgctcgtatt cgcgcaggat gcgaatcgag gcgatgatca gcaggatgat
    12301 cagcgccagg aaaccgagtc cgaagttcag catgccgttc tcctcgtcgt cattcgtcgg
    12361 cgctcgcgcg cgtcgcgtcg agcaccaatc cgtggaccgc cgtgatgcgc aggcgttccc
    12421 cggcgcgcag cggctccttc gtgcgcacgc gccagcgctc gccctgcacc cgcgcccagc
    12481 cctcgcccga gatgtcctcg agcgcctcgc cggacgcgcc gagcaggtac tcgcgcccgg
    12541 cgaccaccgg gcgcgcgcgg ttcttcacca tcatgcccac gaccaggaac acgaaggccg
    12601 cgctgccgac cgtaacgccg gcgatcagcg tgtacgggat ctcgaactgc ggaatgtcgg
    12661 tgtcgagcag catgatcgag ccgatgacga acgccaccag cccgccgacg gcgagcgcgc
    12721 cgtgcgcggc cacccacacc tcggccacca tgaaggcgat gccgagcaag agcagcgcca
    12781 gccccgcgta gttcaccggc agcagctgga acgcgtacat cgcgatcagg atgcagatcg
    12841 cgccgaccac gccgggcagg atcagtccag ggttggagaa ctcgaagatc agcgcgtaga
    12901 tgccgagcag gatcatcagg taggccacgc tcgggttggt gatcacgcgc agcacgttgg
    12961 tgcgccagtc ggcctcgatc tcggtcacct gggcgccggc ggtggcgagc ttgcgcttct
    13021 cgccgcccgc ctcgaccgtg cgcccgtcga ccgcctgcag cagcgccggc acgctgtcgg
    13081 cgacgatctc gatcaccttg atcttcttcg cttccgcggc cgagaggctg accgcctcgc
    13141 gcaccgcgcg ctcgccccac tccgcgttgc gcccgcgcag ctcggcgagg ctgcggatat
    13201 acgcggccgc gtcgttggtc gccttgcgca tcatcgtgtc gccggcgggc gccgcgccct
    13261 tcttggcatc cttgcccgag ggatccggct tgtcgccgcc gcccgagggc gcgccgatcg
    13321 ccaccggcga ggccgcgccg aggttggtcg ccggggccat ggcggcgatg tgcgccgc
//
LOCUS       DBNHKE010000117         2972 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_812991, whole genome shotgun sequence.
ACCESSION   DBNHKE010000117 DBNHKE010000000
VERSION     DBNHKE010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_812991"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..865)
                     /locus_tag="AC16O9_07330"
     CDS             complement(179..865)
                     /locus_tag="AC16O9_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516465.1"
                     /translation="MMRVLLIDDHALVRKGIEELLQSRGVQVVAAVGSGEEGVRRARE
                     LPADLILLDVKMPGMNGIDTLHALRAAGVRAPVVMLTMSREDADLGAALRAGAQGYLL
                     KDIEPEELVPALQAALEGSNVVARELVGSLARLVGGEAGAEPAAPRPAAPFSELTPRE
                     REILERIADGLSNKMIARALDISDGTVKLHVKAILRKLGMRSRVEAAVAAAEHGLGRR
                     RGVPGPGPRA"
     gene            complement(865..2058)
                     /locus_tag="AC16O9_07335"
     CDS             complement(865..2058)
                     /locus_tag="AC16O9_07335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR6516466.1"
                     /translation="MADTRTPKAGAPQAPSVRLARKTQSLDILYAVATSLSQPGTLEQ
                     VLDGFLDTFIELVDARAASVRLATGDGGPRLAASRGLAPEVVERDRRMRAGGGPGSRA
                     AADGRLHLQRGTAACAALIGRPMLERDCVEFVAVPVRYQDRVLGVYNLFLDRPLAAMG
                     EDMPDLLISVGRHLGLAMEKARLEGEARRVAIMEERALIGNELHDSLAQSLIGMRLQA
                     KLLGEYLARNDVGRAQYEATRLRRAVERANDDVRELLTNYRLKIGERGLAHTLEELVA
                     RFRRDTGIAVFFQNECAALELSPAQEIQVFYIIQEALTNIRRHSGARNVRILLNSQDE
                     LYTVLIEDDGLGMDDADAGTTSAEHAGLAIMRERTERLQGQIVIESEPGEGTRIVLMF
                     NAPAR"
     gene            complement(2103..2405)
                     /locus_tag="AC16O9_07340"
     CDS             complement(2103..2405)
                     /locus_tag="AC16O9_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013671.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR6516467.1"
                     /translation="MIKVTPQAAEQILKSAAGACLRLAARLGDKGVIEYGMGFDEKAE
                     GDTQFESAGVQVLVSPGSVELLTGATLDYVELNPGEWRFIFINPNDPSHKAPKPAR"
     gene            complement(2405..2716)
                     /locus_tag="AC16O9_07345"
     CDS             complement(2405..2716)
                     /locus_tag="AC16O9_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR6516468.1"
                     /translation="MTFDEEGYLVDPADWSESVADALARKEGIQLTDAHWTVIRFIRQ
                     YYEAQQIAPDARHVMKHLAEYRGEQGADRNDLFKLFPYGYVKQACKIAGMRRPRAWST
                     G"
     gene            complement(2716..>2972)
                     /locus_tag="AC16O9_07350"
     CDS             complement(2716..>2972)
                     /locus_tag="AC16O9_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516469.1"
                     /translation="GRGRGPARATAVIRAHEFHYSSVENLGPGLEFAYAVERGHGIDG
                     RHDGIVYKNLLASYCHLRDVAEHPWARRFVKFAATAATHA"
BASE COUNT          380 a         1095 c         1046 g          451 t
ORIGIN      
        1 cgacaaggat ctcggctggc gctgggcgat ggcattgctc aagcgcgggc tgctggtcaa
       61 tccgaacgag aagttctacc tctccatcgc gcactccgag gccgacatcg agcacacgct
      121 cggcgtggtc gaggaggcct tcgccgcgct gcgcgccaac gcctgaacgc cccgcggcct
      181 aggcgcgcgg gcccggcccg ggcacgcccc gcctgcggcc cagcccgtgc tcggccgccg
      241 ccaccgccgc ttcgacgcgt gaacgcatgc caagcttgcg caggatcgcc ttgacgtgca
      301 gcttcaccgt gccgtcgctg atgtcgagcg cgcgcgcgat catcttgttg ctcagcccgt
      361 cggcgatgcg ctccaggatc tcccgctcgc gcggcgtgag ctcggagaag ggcgccgccg
      421 gccgcggcgc cgccggctcc gcgccggcct cgccgcccac caggcgcgcc agcgacccca
      481 ccagctcgcg cgccacgacg ttgctgccct cgagcgccgc ctggagcgcg ggcacgagct
      541 cctcgggctc gatgtccttc agcaggtagc cctgcgcgcc tgcgcgcagg gccgcgccga
      601 ggtcggcgtc ttcgcggctc atggtgagca tcaccaccgg cgcgcgcacg ccggccgcgc
      661 gcagcgcgtg cagggtgtcg atgccgttca tgcccggcat cttgacatcc agcaggatca
      721 ggtcggcggg cagctcgcga gcgcggcgca cgccctcctc gcccgagccc accgcggcca
      781 ccacctgcac gccgcggctc tgcagcagct cctcgatgcc cttgcgcacg agcgcgtggt
      841 cgtcgatcag caggacgcgc atcattagcg cgccggcgcg ttgaacatca gcacgatgcg
      901 cgtgccttcg ccgggctcgc tctcgatcac gatctggccc tgcagccgct cggtgcgctc
      961 gcgcatgatg gcgagccccg cgtgctcggc cgaggtcgtc ccggcgtccg cgtcgtccat
     1021 gcccaggccg tcgtcctcga tcagcacggt gtagagctca tcctggctgt tgagcaggat
     1081 gcgcacgttg cgcgcgccgc tgtgcctgcg gatgttggtg agcgcctcct ggatgatgta
     1141 gaagacctgg atctcctgcg cgggcgagag ctcgagcgcc gcgcactcgt tctggaagaa
     1201 cacggcgatg ccggtgtcgc ggcgaaagcg cgccacgagt tcctcgagcg tgtgcgccag
     1261 gccgcgctcg ccgatcttca ggcgatagtt ggtgaggagc tcgcgcacgt cgtcgttggc
     1321 ccgctccacg gcgcggcgca gccgcgtcgc ctcgtactgg gcgcggccga cgtcgttgcg
     1381 cgccaggtac tcgcccagca gcttcgcctg caggcgcatg ccgatcagcg actgcgcgag
     1441 cgagtcgtgc agctcgttgc cgatgagggc gcgctcctcc atgatcgcca cgcggcgcgc
     1501 ctcgccctcc aggcgcgcct tctccatcgc gagcccgagg tgccgcccca cgctgatcag
     1561 caggtcgggc atgtcctcgc ccatcgcggc gagcggccgg tcgaggaaca ggttgtacac
     1621 gccgagcacg cggtcctggt accgcaccgg cacggcgacg aactccacgc agtcgcgctc
     1681 gagcatcggc cggccgatga gcgccgcgca cgccgcggtg ccgcgctgca ggtgcagccg
     1741 cccgtcggcc gcggcgcggc tgcccgggcc gccgccggcg cgcatccggc ggtcgcgctc
     1801 cacgacctcg ggcgcgaggc cgcggctcgc ggcgagccgc gggccgccgt cgccggtggc
     1861 gaggcgcacg ctcgcggcgc gcgcgtccac caactcgatg aaggtatcga gaaagccgtc
     1921 gagcacctgc tcgagcgtcc cgggctggct gaggctggtc gccaccgcgt acaggatgtc
     1981 gagcgactgc gtcttgcgcg caaggcgcac cgagggcgcc tggggcgcgc ccgctttcgg
     2041 tgtccgggtg tctgccatgg cgcggggcag cgtaaccctg cgccgcggtg tcgtccagcg
     2101 cctcagcgcg cgggcttggg cgccttgtgc gacgggtcgt tcggattgat gaagatgaag
     2161 cgccactcgc ccggattgag ctcgacgtag tcgagcgtcg cgccggtgag cagctcgacg
     2221 ctgccgggcg agacgaggac ctggacgccc gcgctctcga actgcgtgtc gccctcggcc
     2281 ttctcgtcga agcccatgcc gtactcgatg acgcccttgt cgcccaggcg cgcggcaagg
     2341 cgcaggcagg cgcccgctgc cgacttcagg atctgctcgg ccgcctgcgg cgtgaccttg
     2401 atcatcagcc cgtgctccag gcgcgcggcc ggcgcatgcc cgcgatcttg caggcctgct
     2461 tcacgtagcc gtagggaaac agcttgaaca ggtcgttgcg gtccgcgccc tgctcgcccc
     2521 ggtactcggc gaggtgcttc atgacgtggc gcgcgtccgg agcgatctgc tgcgcctcgt
     2581 agtactggcg gatgaagcgg atcaccgtcc agtgcgcgtc ggtgagctgg attccctcct
     2641 tgcgcgccag cgcatcggcg acgctctcgc tccagtcggc gggatcgacg aggtagcctt
     2701 cttcgtcgaa cgtcatcagg cgtgggtcgc ggcggtcgcg gcgaacttca cgaagcgccg
     2761 cgcccaagga tgctcggcga cgtcgcgcag gtggcagtag gacgcgagca ggttcttgta
     2821 gacgatgccg tcgtgccggc cgtcgatgcc gtggccgcgc tcgaccgcgt aggcgaactc
     2881 gagccccggg ccgaggttct ccacgctcga gtagtggaac tcgtgcgcgc ggatcacggc
     2941 cgtggcgcgc gcgggccccc gcccccgccc cg
//
LOCUS       DBNHKE010000118         6255 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_390166, whole genome shotgun sequence.
ACCESSION   DBNHKE010000118 DBNHKE010000000
VERSION     DBNHKE010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_390166"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /gene="coaD"
                     /locus_tag="AC16O9_07355"
     CDS             <1..466
                     /gene="coaD"
                     /locus_tag="AC16O9_07355"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR6516470.1"
                     /translation="FDPLTRGHEDLVRRAAKLFDPLIIGVAISRGKSTFFTLQERTEM
                     AREVLGDLKSVQITSFTGLLTDFVRQHDAHVVLRGLRAVSDFEYEFQLAGMNRSLNPD
                     FETLFLTPSEQHMFISATLVREIAILGGDVSKFVHPFVGKRLDAKVKSLGKK"
     gene            complement(471..1280)
                     /gene="mutM"
                     /locus_tag="AC16O9_07360"
     CDS             complement(471..1280)
                     /gene="mutM"
                     /locus_tag="AC16O9_07360"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR6516471.1"
                     /translation="MPELPEVEVTRRGIARALAGRQISAIAVREPRLRWPVPDAVRAL
                     AGRTVRSVKRRAKYLLLDCGDGELIVHLGMSGSLRLLPAGAAFDKHDHFDLVLGEQLL
                     RLRDPRRFGAVLWHAAGGAPHPLLAQLGIEPLSRALDGARLYALTRVHRAAIKVFLMD
                     SRRIVGVGNIYASEALYRARIDPRTPARRLSLARCTRLARAIKDTLRAAIRAGGSSLR
                     DYVGADGGYGCFQLRTLVYDRAGRPCRRCGAAVRRIVQGQRSTYFCPACQR"
     gene            1332..3068
                     /locus_tag="AC16O9_07365"
     CDS             1332..3068
                     /locus_tag="AC16O9_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516472.1"
                     /translation="MPAVNRLLILLFVLPLAACVALAQSDEEPELQAGVPEAAEPERS
                     TAKLPEQELTEPLLYEMLLAEIALQRGNPALAAQTYADLARRTRDPRVARRAVEVANF
                     ARTPQFALEAARIWHDADADSVQALQTLTALLVNARKVDEAEPYLAKLLERDGEAAAG
                     GFMQLSRLLAANPDKPANLRVVRKLAARHPQVPQAQLAIAQAAYAAGDDAAALAAARR
                     AAEMRPGWDAAAIYEAQVLQRKSPELAAASLAAFLGRHPESREARLAYARLLTGEHRY
                     AEARAEFEKLLAAFPDNGDVRYAVGLLALQLKDYATAETSLQRLLEMGFRDPHGVRFM
                     LGQAAEEQKKWAAALRWYESIPHGDSNYLPSRLRIANVMGKQGKLTEARAYLRTLDTE
                     GDQGVQVLIAEAQLLRDANQHRDAFELLGGALKARPEQPELLYDHAITAEKLERFDVL
                     EKSLRTLIRLKPDYAHAYNALGYSYADRNLRLPEARKLIERALELSPEDFYIIDSMGW
                     VLYRMGDLKGAAEHLRRAYHGRPDAEIGAHLGEVLWQMGERAEAERVWQEVQKRNPDN
                     ETLQRTLKRLRP"
     gene            3092..3631
                     /gene="lolB"
                     /locus_tag="AC16O9_07370"
     CDS             3092..3631
                     /gene="lolB"
                     /locus_tag="AC16O9_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015512.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR6516473.1"
                     /translation="MNPILRAFAIAAALAAAGCAELTARAPGESAEFEFNARFAARYR
                     DEAASGQLAWRHDAARDEVLISSPLGQGLARVTRRDGRVTLVTADDRRYSAADAETLT
                     EEVLGFRLPLVGLAHWVRARPAAGAPAQSTYDAAGRLALLEQHGWTIEYQAYEAGRPA
                     RLRLRYPGLELRLAISEWR"
     gene            3628..4485
                     /gene="ispE"
                     /locus_tag="AC16O9_07375"
     CDS             3628..4485
                     /gene="ispE"
                     /locus_tag="AC16O9_07375"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR6516474.1"
                     /translation="MNWFPAPGKLNLFLHVLGRRADGMHELQTVFRLIERGDRVGIQL
                     RGDGAIRRYDPLPGLAEADDLCVRAARLLQAESGAPQGADLALDKRLPIGGGLGGGSS
                     DAATTLIVLNRLWGLHWPRARLLELALRLGADVPAFVFGENALGEGVGERLSALELPA
                     AWYLVLVPPAAVSTRDVFSDNALTRNTKRLKIPPFFSGLGANDLEPVAVKRSPEIAAH
                     LAWLRARRPQARMTGSGACVFAEFASQEEAQGVLGELPGTMRGFVTRGLERHPLFDFL
                     NLLGSRQVG"
     gene            4463..4539
                     /locus_tag="AC16O9_07380"
     tRNA            4463..4539
                     /locus_tag="AC16O9_07380"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4497..4499,aa:Gln,seq:ttg)
     gene            4606..5538
                     /locus_tag="AC16O9_07385"
     CDS             4606..5538
                     /locus_tag="AC16O9_07385"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR6516475.1"
                     /translation="MVFTGNANPRLAQDVAKHLNMSLGRAICGRFSDGEVMVEIMENV
                     RGKDVFVLQSTCAPTNDNLMELLVMVDALKRSSAARVTAAIPYFGYARQDRRPRSARV
                     AISAKVVANMLTSVGVARVLTMDLHADQIQGFFDMPVDNVYAAPVLLGDVWKQGYKDL
                     IVVSPDVGGVVRARALAKRLESDLAIIDKRRPKANLSEVMNVIGEVQGRSCVIMDDMV
                     DTAGTLVKAAQVLKEEGATKVVAYCTHAVLSGAAVERIDNSDLDELVVTDTIPLREDA
                     RASAKIRQLSVAGLLAETILRIFNEDSVSSLFIE"
     gene            5597..>6255
                     /locus_tag="AC16O9_07390"
     CDS             5597..>6255
                     /locus_tag="AC16O9_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR6516476.1"
                     /translation="MKFEINASQRKVQGTGASRRLRRAGRVPGILYGGDKGAVNIELD
                     HKDLLRNLHNEKFHASILTLQLDGGAEQVLLRAFNMHPVKSQVQHVDFQRVSKDKKIH
                     VKVPLHFVNAAGSPGVKEQAGVITHVLNELDIECFPDDLPEFIEVDLAQITVGSSVHA
                     RELKLPNGVEALLHKDDDPVVATCTLPALVSEEEEVAAEALPAAGEVPTTEQAAAEKP
                     G"
BASE COUNT          974 a         2196 c         2195 g          890 t
ORIGIN      
        1 ctttgatccg ctcacgcgcg gccatgaaga cctggtgcgg cgcgcggcga agctgttcga
       61 cccgctcatc atcggcgtcg ccatcagccg cggcaagagc accttcttca cgctgcagga
      121 gcgcaccgag atggcgcgcg aggtgctcgg cgatctgaag agcgtgcaga tcacgagctt
      181 caccggcctg ctcaccgact tcgtgcgcca gcacgatgcc cacgtggtgc tgcggggcct
      241 gcgcgcggtg tccgacttcg agtacgagtt ccagctcgcc ggcatgaacc ggagcctcaa
      301 cccggatttc gagaccctgt tcctgacgcc ctccgagcag cacatgttca tctcggcgac
      361 gctggtgcgc gagatcgcga ttctgggcgg cgacgtctcc aagttcgtgc atcccttcgt
      421 cggcaagcgg ctcgacgcga aggtgaagtc cctcggcaag aagtagcgcg ctagcgctgg
      481 caggcggggc agaagtaggt cgagcgctgc ccctgcacga tgcgccgcac cgccgcgccg
      541 cagcgccggc aggggcggcc cgcacggtcg tagaccaggg tgcgcagctg gaagcagccg
      601 tagccgccgt cggcgccgac atagtcgcgc agcgacgaac cgccggcgcg gatcgcggcg
      661 cgcagcgtgt ccttgatggc gcgcgcaagg cgcgtgcagc gcgcaagcga caggcgccgc
      721 gccggcgtgc gcgggtcgat ccgcgcgcgg taaagcgcct cgctcgcata gatgttgccc
      781 acccccacga tgcgccggct gtccatcagg aagaccttga tcgcggcacg gtgcacgcgc
      841 gtcagcgcgt agaggcgcgc gccgtcgagc gcgcgcgaca acggctcgat tcccagttgc
      901 gcgagcagcg gatgcggcgc cccgcccgcc gcgtgccaca gcaccgcgcc gaatcgccgc
      961 gggtccctga ggcgcagcag ctgctcgccg agcaccaggt cgaagtggtc gtgcttgtcg
     1021 aacgcggcgc ccgcaggcag caggcgcagg ctcccgctca tgccgaggtg cacgatgagc
     1081 tcgccgtcgc cgcagtcgag cagcaggtac ttggcgcggc gcttcaccga gcgcacggtg
     1141 cgcccggcga gggcgcgcac tgcgtcgggc accggccagc gcagccgcgg ctcgcgcacc
     1201 gcgatggcgg agatttgccg gccggcgagc gcgcgcgcga tgccgcgccg cgtgacttcg
     1261 acttcgggta gctcgggcat ggaactttgt tgcttgtgca gggcaccaaa tatacctaca
     1321 ctctgccggc catgcccgcg gtaaatcgcc tgctgatcct gctctttgtg ctgccgctcg
     1381 cggcctgcgt ggcgctcgcg cagtctgacg aggagcccga gttgcaggcg ggcgtcccgg
     1441 aagccgccga gcccgagcgc tccaccgcga agctgccgga gcaagagctg accgagccgc
     1501 tgctctacga gatgctgctc gccgagatcg cgctgcagcg cggcaatccc gcgctggcgg
     1561 cgcagaccta cgccgatctc gcccggcgca cgcgcgatcc gcgcgtcgcg cgccgcgcgg
     1621 tcgaggtcgc caacttcgcg cgcacgccgc agttcgcgct ggaggcggcg cgcatctggc
     1681 acgatgccga cgccgactcg gtgcaggcgc tgcagacgct gaccgcgctg ctggtaaacg
     1741 cgcgcaaggt ggacgaagcc gagccctacc tcgccaagct gctcgagcgc gacggcgagg
     1801 cggcggcggg cggcttcatg cagctgtcgc ggctgctcgc cgcgaacccc gacaagcctg
     1861 ccaacctgcg cgtggtgcgc aagctcgcgg cgcgccatcc gcaggtgccg caggcgcagc
     1921 tcgcgatcgc gcaggcagcg tatgccgcgg gcgacgatgc cgcggcgctc gccgcggcgc
     1981 gccgcgccgc cgagatgcgc cccggctggg acgcggccgc catctacgaa gcgcaggtgc
     2041 tgcagcgcaa gtcgcccgag ctcgccgcgg cctcgctcgc cgcgttcctc ggccggcacc
     2101 ccgagtcgcg cgaggcgcgc ctcgcctacg cgcggctgct caccggcgag caccgctatg
     2161 ccgaggcgcg cgcggagttc gagaagctgc tcgccgcgtt tcccgacaac ggcgacgtgc
     2221 gctacgcggt ggggctgctg gcgctgcagc tgaaggatta cgcgaccgcc gaaacgagcc
     2281 tgcagcgcct gctcgagatg ggctttcgcg atccgcacgg ggtgcgcttc atgctggggc
     2341 aggcggccga ggagcaaaag aagtgggccg ccgccttgcg ctggtacgag agcatcccgc
     2401 acggcgacag caactacctg ccttcgcgcc tgcgcatcgc caacgtgatg ggcaagcagg
     2461 gcaagctcac ggaagcgcgc gcgtacctgc gcacgctcga caccgagggc gaccagggcg
     2521 tgcaggtgct gatcgcggag gcgcagctgc tgcgcgacgc gaaccagcac cgcgacgcct
     2581 tcgagctgct cggcggcgcg ctcaaggcgc gtcccgagca gcccgagctg ctctacgatc
     2641 acgccatcac cgccgagaag ctcgagcgct tcgacgtgct cgaaaagagc ctgcgcacgc
     2701 tgatccggct gaagccggat tacgcgcacg cctacaacgc gctcggctac tcgtacgccg
     2761 accgcaacct gcgcctgccc gaggcgcgca agctcatcga gcgcgcgctc gagctgtcgc
     2821 cggaggactt ctacatcatc gacagcatgg gctgggtgct ctaccgcatg ggcgacctga
     2881 agggcgctgc cgagcacctg cgccgcgcct accacggccg cccggatgcc gagatcggcg
     2941 cgcacctcgg cgaagtgctg tggcaaatgg gcgagcgcgc cgaagccgag cgcgtgtggc
     3001 aggaagtgca gaagcgcaat cccgacaacg agacgctgca gaggaccctg aagcgcctgc
     3061 gcccctagcg cgcggcgggc gggtgccagg cgtgaacccg atcctgcgtg cctttgccat
     3121 cgccgccgcg ctcgccgccg ccggctgcgc cgagctcacg gcgcgcgcgc ccggcgagag
     3181 cgccgaattc gagttcaatg cgcggttcgc cgcgcgctac cgcgatgagg cggcctcggg
     3241 gcagctggcg tggcgccacg acgcggcgcg cgacgaggtg ctgatcagct cgcccctggg
     3301 ccaagggctg gcgcgcgtga cgcgccgcga tggccgcgtc accctggtca ccgccgacga
     3361 ccggcgctac tcggccgccg acgccgaaac cctgaccgag gaagtgctgg gctttcgcct
     3421 gccgctcgtc ggcctcgccc actgggtgcg cgcgcggccc gcggcaggcg caccggcgca
     3481 atccacctac gacgcggccg ggcgcctcgc cctgctcgag cagcacggct ggacgatcga
     3541 gtaccaggcc tacgaggccg ggcgcccggc gcggctgcgc ctgcgctacc cggggctcga
     3601 gctgcgcctc gccatttccg agtggcgatg aactggtttc ccgcgccggg gaagctgaac
     3661 ctgttcttgc acgtgcttgg ccgccgcgcc gacggcatgc atgagctgca gaccgtgttc
     3721 cgcctcatcg agcgcggcga tcgcgtcggc atccagctgc gcggcgacgg cgcgatccgg
     3781 cgctacgacc cgctgcccgg cctcgccgag gccgacgacc tgtgcgtgcg cgccgcgcgc
     3841 ctcctgcagg ccgagagcgg tgcgccgcag ggtgccgatc tcgcgctcga caagcgcctg
     3901 ccgatcggcg gcggcctcgg gggcggctcc tcggacgccg ccaccacgct catcgtgctg
     3961 aaccgcctgt ggggcctgca ctggccgcgc gcgcggctgc tcgagctcgc gctgcgcctc
     4021 ggggccgacg tgccggcgtt cgtcttcggc gagaatgcgc tcggcgaggg cgtcggcgag
     4081 cgcctgagcg cgctcgagct gcccgcggcg tggtacctgg tgctggtgcc gccggcggcc
     4141 gtttccacgc gcgacgtgtt cagcgataac gcgttgacac gtaatacgaa acgactcaaa
     4201 ataccgccct ttttctccgg cctgggcgca aacgatctcg aacccgtggc cgtgaagcgc
     4261 agcccggaga ttgcggcgca cctcgcgtgg ttgcgcgcgc ggcgcccgca agcgcggatg
     4321 acaggatcgg gggcgtgcgt gttcgcggag ttcgcgtcgc aggaagaagc gcaaggcgtg
     4381 ctgggcgagc tgcccggcac gatgcgcggt ttcgtcacgc ggggactcga gcgtcacccg
     4441 ttgtttgatt tcttgaacct tttggggagt cgccaagttg gttaaggcac cggattttga
     4501 ttccggcatt cgtaggttcg agtcctacct ccccagccat ttgcgccgcg caacgtcatc
     4561 gtgaaccgca tcgccaccgc ccccgcctcc gtgatggacc gcctgatggt gttcacgggc
     4621 aacgccaacc cgcggctcgc gcaggacgtc gccaagcacc tcaacatgtc gctcgggcgc
     4681 gcgatctgcg ggcgcttctc ggacggcgag gtgatggtcg agatcatgga gaacgtgcgc
     4741 ggcaaggacg tgttcgtgct gcagtcgacc tgcgcgccga ccaacgacaa cctgatggag
     4801 ctgctggtga tggtggatgc gctcaagcgc tcctccgccg cgcgcgtcac ggccgccatt
     4861 ccctacttcg gctacgcgcg ccaggaccgc cgcccgcgct cggcgcgcgt ggccatcagc
     4921 gccaaggtgg tggccaacat gctcacctcg gtgggcgtgg cgcgcgtgct caccatggac
     4981 ctgcacgccg atcagatcca gggctttttc gacatgccgg tggacaacgt gtacgcggcg
     5041 ccggtgctgc tcggcgacgt ctggaagcag ggctacaagg atctgatcgt ggtgtcgccg
     5101 gacgtcggcg gcgtggtgcg cgcgcgcgcg ctcgccaagc ggctggaatc ggacctggcg
     5161 atcatcgaca agcgccgacc gaaggccaat ctctcggaag tgatgaatgt catcggcgaa
     5221 gtgcagggcc gcagctgcgt catcatggac gacatggtgg acaccgccgg cacgctggtg
     5281 aaggcggcgc aggtgctgaa ggaagagggc gccaccaagg tggtcgccta ctgtacgcac
     5341 gccgtgctct ccggggccgc ggtcgagcgc atcgacaatt cggacctcga cgagctggtg
     5401 gtcaccgaca ccattccgct gcgcgaggat gcgcgcgcaa gcgccaagat ccgccagctg
     5461 tcggtggccg gcctgctcgc cgagaccatt ctgcgcatct tcaacgagga ctcggtgagt
     5521 tctctattca tcgaatagga tcatcaaccc cggtggggcc tggtcgcggg accgccggca
     5581 cgacaaggag ctgtcaatga aattcgagat caacgccagc caacgcaagg tgcaaggtac
     5641 gggtgcgagc cgccgcctgc gccgcgcggg ccgcgtgccc ggaatcctct acggcggcga
     5701 caagggcgcc gtgaatatcg agctcgacca caaggacctg ctgcgcaacc tgcacaacga
     5761 gaagttccac gcctcgatcc tgacgttgca gctcgacggc ggcgccgagc aggtgctgct
     5821 gcgcgccttc aacatgcacc cggtcaaatc gcaggtgcag cacgtggact tccagcgcgt
     5881 gtccaaggac aagaagatcc acgtgaaggt gccgctgcac ttcgtcaatg ccgccggctc
     5941 gcccggggtg aaggagcagg ccggcgtcat cacgcacgtg ttgaacgagc tcgacatcga
     6001 gtgctttccc gacgacctgc cggagttcat cgaggtcgac ctggcgcaga tcacggtcgg
     6061 cagctcggtg cacgcgcgcg agctcaagct gccgaacggc gtcgaggcgc tgctgcacaa
     6121 ggacgacgac ccggtggtcg ccacctgcac gctgccggcg ctggtcagcg aggaggaaga
     6181 agtcgccgcc gaggccctac cggccgcggg cgaagtgccg accaccgagc aggcggcggc
     6241 ggagaagccg ggcga
//
LOCUS       DBNHKE010000119         4702 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_284694, whole genome shotgun sequence.
ACCESSION   DBNHKE010000119 DBNHKE010000000
VERSION     DBNHKE010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_284694"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC16O9_07395"
     CDS             complement(<1..581)
                     /locus_tag="AC16O9_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516477.1"
                     /translation="MLYARTFLQVLKSAGQFRVRLNALQLAAAAVALAASGHAAAAPG
                     TCPAGGRTLTADVVAFDQPLMYNRLGASNINGMMFALKRDAVFVDRGNPGDSDHLKPL
                     TQVTRSGLELAGNVELRPDKRPRPLVLRVAAGDCLTVNFTNLLTPLANPRKTPIPGEP
                     PFNVIIDDQPESRFAGFHVSGMQLVGSIADDSS"
     gene            1058..2371
                     /locus_tag="AC16O9_07400"
     CDS             1058..2371
                     /locus_tag="AC16O9_07400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6516478.1"
                     /translation="MRGTETGCRLRATLRQHLLVRMVVLVSLVALALGTCELLEIHIP
                     LAPVVVIALLMAALSAAAYVRLRRTERIGEAEIFGHILLDVAALTALLYFTGGVYNPI
                     AYCYLVLVLYGATALPQRFGWALAAASVLCFSLLQIFYVPLPLPDTLAAHAGLDEMAR
                     WVVYVMLAGLVLWFALRLNELRQSHYRHIEAEAEEHARERYLVGLATLAAGTAHEMGT
                     PLSTMSVVVGDLRRGGQPPADWKEQIDLLWRQLLLCKHSLSGLARASDLAQLGKTHVV
                     PARAFIEGLAKRFHLLRPEVPLSLHYAPDDNALALRVDLSLEQALLSLLNNAAQASPD
                     AVDLRVSAEGAMLEIDILDRGPGISPQLRERLGRGPVGARPGGHGLGLLIANSAIERL
                     GGSVLLRDREGGGTCARVELPLHAAAPAAPAAGRDYSKGASVAAA"
     gene            2358..2900
                     /locus_tag="AC16O9_07405"
     CDS             2358..2900
                     /locus_tag="AC16O9_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6516479.1"
                     /translation="MQQPERRNLLLVDDNENFCWAMQRSFAQRGYEVRVAHSVANASA
                     LLEQWAPDYAVVDLRMPGQAGLALLPRILQANAEARTVVLTGYASIATAVEAIKLGAT
                     HYLVKPVDADTVEAAFKRTEGDEAVGPSERELSVDRLEWEHIQRVLADNDGNISATAR
                     ALSMHRRTLQRKLNKHPVRR"
     gene            complement(3021..>4702)
                     /locus_tag="AC16O9_07410"
     CDS             complement(3021..>4702)
                     /locus_tag="AC16O9_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR6516480.1"
                     /translation="RPLMEVFEVAGRDPHRQRFRADHDGLDGMLDSVRIVISGGMLTI
                     TNVTSGAVIFSGHLRDFASAYFTSNPEHLPKRGPRLAAPTGVTALGGDGQVTISWDPV
                     ENATSYDLFYMERGAVAAALRLDDDDEDDEEEFEDEYEDEEEYEHDRNARWIMNVTSP
                     YVLTGLAPDATYGLMVRARDNPRRGPPSAMVFATTSGSAGVPALPQAPTGVTATGGTR
                     QVSVDWAPVTGATSYNLYWSATDGVPPAGRTMVTGASRPAVLTGLADGVTYYAFVTAV
                     NGAGEGARSVQVAATTLPAAPPPATAPAAPVGVAALGGDGQVTLTWPAVSGATSYNIY
                     WSDMTGVTPLSGELIAAATSPYVHSGRMASTTYYYVVTAVNSVGPSLPSAQASATTNA
                     PPPAVPAAPSGIMATGGSNQVTVSWNPVSGATSYNLYWSTTLGVTPATGTKVAGATSP
                     YVHTGLADGTAYYYVVTAANGTVEGPASAQATATTQTAPPPLIDGAALYTTYCAGCHG
                     ALPGEYQGASAQLITSGIAGVGAMRTRFNPTSGSLIQLTPEQIAAIAAAMQ"
BASE COUNT          672 a         1606 c         1697 g          727 t
ORIGIN      
        1 cagctcgagt cgtccgcgat cgacccgacg agctgcatgc cgctcacgtg aaagccggcg
       61 aagcggctct cgggctggtc gtcgataatg acgttgaacg gcggctcgcc ggggatcggg
      121 gttttgcgcg gattggcgag cggcgtcagc aggttggtga aattgacggt caggcagtct
      181 ccggccgcca cgcgcagcac cagcggacgg ggacgcttgt cggggcgcag ctcgacgttg
      241 ccggcgagct ccagacccga gcgggtgacc tgcgtgaggg gtttcaggtg atccgagtcg
      301 cccgggttgc cgcggtcgac gaacacggca tcgcgcttga gcgcgaacat catgccgttg
      361 atgttcgacg ccccgaggcg gttgtacatc agcggctggt cgaacgcgac gacatccgcg
      421 gtgagcgtcc gtccgccggc cgggcatgtg cccggcgcag cggcggcgtg ccccgaggcg
      481 gcgagcgcca cggcggcagc agcgagctgc agtgcgttca ggcgaacgcg gaattgaccg
      541 gcggatttca gaacctgcag aaacgtacgg gcgtaaagca ttgaggtccc cccccaagga
      601 gatcgcaaga tggggcgaag actaggccgc tccgcgcgtg cgtgaaatat gtcacagtgt
      661 cgcgcggcag attgacgcag gataaatggc gggcacgtca agccgggcgc aagtcggcag
      721 tccgggcccg gggggggggg acccaccctt tcagcccccg cagcccggaa ttcctccttt
      781 tgtaagaaag agttcgggca aacaatcgtc agatttctgt ttaccccctt gtcctgcatc
      841 aaagtaggtg cgataacttg tcgcatgctt ttcggcataa ggctgcgtcg gtagcccggg
      901 ggatcccgcc ggaacggtcg cggggcctgg cggaccggcc ggcgcaagag ttgggggcgg
      961 gcagttcgcc cgttgcagtg caaggcgcag gcacgcccgg gggcgtgaag gcgggggttt
     1021 cgaaaacgtg tcggcgattt cgggggaaag gcaggaaatg cggggaactg aaaccggctg
     1081 caggctgcgc gcgacgctgc gccagcacct gctggtgcgc atggtggtgc tggtctcgct
     1141 ggtcgcgctg gcgcttggca cctgcgagct gctcgagatc cacattccgc tcgcgccggt
     1201 ggtggtgatc gcgctgctga tggcggcgct cagcgcggcg gcctacgtgc ggctgcgccg
     1261 gacggagcgc atcggcgaag ccgagatctt cggccatatc ctgctcgacg tcgctgcgct
     1321 caccgcgctg ctgtacttca ccggcggcgt gtacaacccg atcgcgtact gctacctggt
     1381 gctcgtcctg tacggcgcga cggcgctgcc gcaacgcttc gggtgggcgc tcgccgccgc
     1441 gtccgtgctg tgcttttcgc tgctgcagat cttctacgtg ccgctgccgc tgcccgacac
     1501 gctggccgcg cacgccgggc tcgacgagat ggcgcgctgg gtcgtgtacg tgatgctcgc
     1561 aggcctggtg ctgtggttcg cgctgcgcct gaacgagctg cggcaatcgc actaccgcca
     1621 catcgaggcc gaggccgagg agcacgcgcg cgagcgctac ctggtcggcc tggcgacgct
     1681 cgccgcgggc accgcgcacg agatgggcac gccgctgtcg acgatgtcgg tggtggtcgg
     1741 cgacctgcgg cgcggcgggc agccgccggc cgactggaag gagcaaatcg acctgctctg
     1801 gcggcagctg ctgctgtgca agcattcgct ctcgggcctg gcgcgcgcct cggatctcgc
     1861 gcagctcggc aagacgcacg tcgtgccggc gcgcgcgttc atcgagggcc tcgccaagcg
     1921 cttccatctg ctgcgccccg aggttccgct gagcctgcac tacgcgcccg acgataacgc
     1981 gcttgcgctg cgggtggacc tctcgctcga gcaggcgctg ctcagcctcc tcaacaacgc
     2041 cgcgcaggcc tcacccgacg cggtggacct gcgcgtgagc gccgagggcg ccatgctcga
     2101 aatcgatatc ctcgaccgcg gcccgggcat ttctccgcag ctgcgcgagc gcctgggccg
     2161 gggcccggtc ggcgcgcggc ccggcgggca cggcctgggc ctgctgatcg ccaacagcgc
     2221 gatcgagcgc ctgggcggca gcgtgctgct gcgcgaccgc gaaggcggcg gcacctgcgc
     2281 gcgggtggag ctgccgctgc acgcggcggc gccggccgca ccggccgccg gccgcgacta
     2341 cagcaaggga gcgtccgttg cagcagcctg aacgccgcaa tctcctgctc gtcgacgaca
     2401 acgagaactt ctgctgggcg atgcagcgct cgttcgcgca gcgcggctac gaggtgcgcg
     2461 tggcgcactc ggtggccaat gcgagcgcgc tgctggagca gtgggcgccg gattacgcgg
     2521 tggtcgacct gcgcatgccc gggcaggcgg gcctcgcgct cctgccgcgc atcctgcagg
     2581 cgaacgcgga ggcgcgcacc gtggtgctca cgggttacgc cagcatcgca accgcggtcg
     2641 aggcgatcaa gctcggcgcc acccactacc tcgtcaagcc ggtggacgcg gacacggtcg
     2701 aggcggcgtt caagcgcacc gagggcgacg aggccgtggg gcccagcgag cgtgagctct
     2761 cggtggatcg gctcgaatgg gagcacatcc agcgcgtgct ggccgacaac gacggcaaca
     2821 tctcggccac ggcgcgcgcg ctctcgatgc accggcgcac gctgcagcgc aagctgaaca
     2881 agcacccggt gcggcgctag ccggcgcgat cagcgccgcg gtctggccaa gccctgatca
     2941 gccgcgccgg gcccgcgagc ggcttggcgc ggcgccggga gaacgggagg gtcccgccgg
     3001 gaccctccca tcgcctcgcg ctactgcatg gctgcggcga tcgcggcgat ctgctccggc
     3061 gtgagctgga tgaggctccc gctggtcgga ttgaaccgcg tgcgcatggc gccgacgccg
     3121 gcgatgccgc tggtgatcag ctgggcgctc gccccctggt actctcccgg cagcgcgccg
     3181 tgacagcccg cgcagtacgt cgtgtagagc gctgccccgt cgatcagcgg cggcggcgcc
     3241 gtctgcgtgg tcgcggtcgc ctgtgccgat gcaggaccct ctaccgtgcc gttcgccgcg
     3301 gtgacgacgt aatagtaggc cgtgccgtcg gcgagcccgg tgtgaacgta aggcgacgtc
     3361 gcacccgcga ccttggtacc cgtggcgggg gtgaccccca gggtggtgga ccagtacagg
     3421 ttgtacgagg ttgccccgga taccgggttc cacgacacgg tcacctgatt gctgcccccc
     3481 gtcgccatga tgccggaggg cgccgccgga acggccggcg gcggcgcgtt ggtcgtcgca
     3541 ctcgcctgcg cggacggcag gctcgggccc acgctgttca ccgccgtgac cacgtagtag
     3601 tacgtcgtgc tggccatcct gccgctatgc acgtaggggc tcgtcgccgc ggcgatgagc
     3661 tctccgctta agggcgtcac gcccgtcatg tccgaccagt agatgttgta cgacgtggcc
     3721 ccggacaccg ccggccaggt gagcgtgacc tgcccgtcgc cgcccagggc ggcaaccccg
     3781 accggggcgg cgggtgccgt cgccggcggc ggtgcggccg gcagcgtcgt cgccgccacc
     3841 tgcaccgagc gggcaccttc tcccgcgcca ttcacggccg tgacgaaggc gtagtacgtc
     3901 acgccgtcgg caaggccggt gagcaccgcc ggccgcgacg caccggtcac catggtcctg
     3961 ccggccggcg gcacgccgtc ggtcgcggac cagtacaggt tataggacgt cgctcccgtc
     4021 accggcgccc agtcgacgct gacctggcgc gtgccgcctg ttgccgtgac gccggtgggc
     4081 gcctggggca gcgcaggcac acccgcgctg ccgctggtgg tcgcgaagac catggccgag
     4141 ggcggtccac gccgcgggtt gtcgcgcgcg cgcaccatca atccgtaggt cgcatccggc
     4201 gcaaggccgg taagcacgta cgggctcgtc acgttcatga tccaccgcgc gttgcggtcg
     4261 tgctcgtatt cctcctcgtc ctcgtattcg tcctcgaatt cctcctcgtc gtcctcgtcg
     4321 tcatcgtcga gccggagcgc cgccgcgacg gcgccccgtt ccatgtagaa caggtcatag
     4381 gacgtcgcgt tctcgaccgg gtcccatgag atcgtcacct gcccgtcgcc gccgagcgcc
     4441 gtgacgcccg ttggcgccgc cagccgggga ccgcgcttgg gcaggtgctc gggattgctc
     4501 gtgaagtacg cgctcgcgaa atcccgcagg tggccgctga agatgaccgc gcccgaagtc
     4561 acgttggtga tcgtgagcat gccgccggag atcacgatgc gcacgctgtc gagcatgccg
     4621 tcgagtccgt cgtggtcggc gcgaaagcgc tgccgatgcg gatcgcggcc ggccacctcg
     4681 aagacttcca tcagcggccg ca
//
LOCUS       DBNHKE010000120        37521 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_73091, whole genome shotgun sequence.
ACCESSION   DBNHKE010000120 DBNHKE010000000
VERSION     DBNHKE010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 37521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 37521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_73091"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="AC16O9_07415"
     CDS             complement(<1..509)
                     /locus_tag="AC16O9_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6516481.1"
                     /translation="MRLRQAGPLAAWLAGLAVAGALAATARYTADLSAFLPRAPTPAQ
                     QLLVDQLREGAVGRVMLMAIEGADSAARARLSRELAERLRADARFAYVGNGAMQGSER
                     ERTILFEHRYLLSPAVTPERFSAAGLREAIGDTLDLVASSAGALAQALLARDPTGEMA
                     RLAAAQLAEG"
     gene            complement(506..1075)
                     /locus_tag="AC16O9_07420"
     CDS             complement(506..1075)
                     /locus_tag="AC16O9_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HIR6516482.1"
                     /translation="MRLSLFALLLLATPGVWPAPVWTLEQLLQGFAQRAQARASFVEK
                     KTVRVLEQPLVSSGELRFEAPDRLEKRTLRPRPEVLRLHGDTLTLERGERRLQLDLRS
                     HPEAGAFVESIRATLAGDREALERIYAVRLEGSERSWALTLTPRDTGMANLVARIRIA
                     GQGAEVTVIEIAQANGDSTLMSVQRLGAP"
     gene            complement(1065..1871)
                     /locus_tag="AC16O9_07425"
     CDS             complement(1065..1871)
                     /locus_tag="AC16O9_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR6516483.1"
                     /translation="MRWIALGLGRRAARLVLHPVSAYFLLAVPRARRASRAYLSRSLG
                     RRATLAELYRHFYAFAATVLDRVYLLNGRFDLFDIEVRDPPSPGAGELLMGAHLGSFE
                     ALRAAGRQRQGLQVSMVMYEDNARMLNATLAALSPAARQDVIALGQVDSMLRVRDCLA
                     AGGMAGVLADRALHDDDTCTLPFLGAPARFPLGPMRMAALLRCRVTFMAGLYRGGNRY
                     VLQFEPLADFGAAAPAPEAALAAYVACLERHCRAAPYNWFNFYDFWADAA"
     gene            complement(1925..2197)
                     /locus_tag="AC16O9_07430"
     CDS             complement(1925..2197)
                     /locus_tag="AC16O9_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApeI family dehydratase"
                     /protein_id="HIR6516484.1"
                     /translation="MSEARLRFSAAHPAFAGHFPGAPMVPGALLLAVALDALGLARPG
                     LEIASAKFLHPVPPEIEVEVRRTGNRIELRAGDRVVATAQLKTAAA"
     gene            complement(2194..3534)
                     /locus_tag="AC16O9_07435"
     CDS             complement(2194..3534)
                     /locus_tag="AC16O9_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6516485.1"
                     /translation="MTAVPLIAGAAAGSAVAYRDGRALSAAQFVADATRLAGQLPARR
                     HVLNACTDRYRFATGLAAALLRGQVTLLPPDHTPQTVQRLREFAADAYLLADDATVQI
                     DLPCVPAPAEPAPLPPTFAVPEIAHDQLCAIVFTSGSTGTPVPNRKHWGTLSRNVAGA
                     LERWALAGARPAILGTVPPQHMYGFESTVLAALAGGGALCAERPFDPEEICAALARLP
                     RPRVLVTAPFHLRTLLDAVAQPPAADLLLCATALLEAELAALAEARFGAPLLEIYGCT
                     ETGQMATRRTARELEWRTLPGIRVELRDAVAWASGGHVEQPTALGDVLEPLAPDRFLL
                     RGRLADMVNVAGKRSSLAHLDAQLRAIDGVTDGVFYMPDDGAAGGVTRLAAFVVAPGH
                     SRETLLAALRQRIDAAFLPRPLVLLERLPRAASGKLPREALRALARSHGVERAP"
     gene            complement(3553..4098)
                     /locus_tag="AC16O9_07440"
     CDS             complement(3553..4098)
                     /locus_tag="AC16O9_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG4648 family protein"
                     /protein_id="HIR6516486.1"
                     /translation="MSRARTRGWLALLIALALGYAVLAHWLTTAPGRAALGGLGPYVP
                     WFYFLQHVAMFLALAAWFGASLRPGREALVTRFALLVEGTLSPAGLAYTRGATLAWAV
                     FSAAIATASALLFFLAPLALWSVFANLLTLPLVGAMFVAEFLVRVRVCPELSHGGVLR
                     GVTRSVRAYWDSGARTTAGPR"
     gene            complement(4095..4394)
                     /locus_tag="AC16O9_07445"
     CDS             complement(4095..4394)
                     /locus_tag="AC16O9_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIR6516487.1"
                     /translation="MASIPVSTQFAPAADPALLEELAGLITAELNLDVAPSDIAAEAP
                     LYGEGLGLDSIDILELALVVSRRYGVRLRSDHEDNTVIFASLSNLARFIAQNRTK"
     gene            complement(4433..5689)
                     /locus_tag="AC16O9_07450"
     CDS             complement(4433..5689)
                     /locus_tag="AC16O9_07450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR6516488.1"
                     /translation="MTARRVAVTGVGMVSPLGATAQASFEALLAGRSGLRLHAPADAP
                     KPLSLPAGVCADFDAVAAFGRARAGTLDRYSQLGIAAGLEAWRDAGLEPAASEDRESW
                     GIAWGTGVGGAMVFERGYRDLFISGRERLPPLTVVQAMNNACASHLAIQLALGGCCAT
                     YSVACASGAMAIGDAFRRVRSGTAPVMLAGGSEAPLAYGVIRAWLALQVLAPGDAESA
                     PLASKPFQRERAGLVLAEGGAALVLEDWARAERRGARIYAEISGYGESCDHGNLVRPS
                     SEGQARALRAALADAAMTPLEVDYVNAHATGTREGDAIEVAALAAALGGRAAEVAVSA
                     TKSAHGHTLGAAGALAAAFTVLGLRNGAIPPTLGDAADLDPECAGVLHVTGAARRDLP
                     LRAALTNAFAFGGTNAVLAFRAPQPV"
     gene            complement(5686..5922)
                     /locus_tag="AC16O9_07455"
     CDS             complement(5686..5922)
                     /locus_tag="AC16O9_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6516489.1"
                     /translation="MADPAPAYRYIQERLGVDVKSADAATELASLGVDSLSLLEMVFE
                     IEERFGVKVDEDTPPPKSCGELVALIERLAAAKS"
     gene            complement(5927..6889)
                     /locus_tag="AC16O9_07460"
     CDS             complement(5927..6889)
                     /locus_tag="AC16O9_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR6516490.1"
                     /translation="MKRIFAALFAVLAFGAHAQYPTRPITMIVPFPPGGVADIVGRPL
                     AAAMEKSLGQPVVVVNRTGAGGAIGMTAAAKADPDGYTILMALSSISIFPVSDPLQGK
                     PAPYQISDFVPIALVTADPTVLVVGGDSPHKTVADFVAAAKANPGKINYSSSGIYGTL
                     HVAMEMFANAAGIKLFHVPYQGGGPAVRALLSGEVQALASGPAAAIGQIQAGKMRALA
                     GWGAKRLELMPALPTFKELGYQDVEFYIWAGVVAPAATPAEAQHKLRAAVREAVATPL
                     FSGAMEKVRTPINYLDAPEFKDFWFEDAKRLRAAVLKIGAVAGK"
     gene            complement(6886..7686)
                     /gene="fabI"
                     /locus_tag="AC16O9_07465"
     CDS             complement(6886..7686)
                     /gene="fabI"
                     /locus_tag="AC16O9_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR6516491.1"
                     /translation="MTLLDGRRILITGLISNRSIAYGVAKAARREGATLAFTYQNERF
                     KQRVEEMAHEFGSDIALPCDVSEDAQIEALFAQLGKRWDSLDGLVHAIAYAPRETLDG
                     ELLDGLSREAFRTALDISAYSFPALAKAALPMMKGGKGALVTLSYLGAARVVPHYNVM
                     GLAKASLEASVRYLAANLGPRGIRVNGISAGPIKTLAAAGIAGMGRLLKFVAEHAPLR
                     RNVTTEDVGNAAVFLLSDLAAGVTGEVLYVDSGFRNVVAGIPGGGSES"
     gene            7710..9788
                     /locus_tag="AC16O9_07470"
     CDS             7710..9788
                     /locus_tag="AC16O9_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516492.1"
                     /translation="MRPILLLACLAAAGCGDVWNDPYPASDRGKNILYSAFTERPKHL
                     DPVQSYTEDEARFTQQVYEPPLQYHYLKRPYELVPLTALAVPKAAPAPGGGVVYTIRI
                     RRGIRYQPHPAFVEANQALAPERIARLDSPYRLPLGTRELTADDYIYEIKRLASPRLH
                     SPILGLMAEKIVGLAEFAERLKKFDTRKQDWLDLRKHRLEGVEKVDDHTYRVRLKEPY
                     PQFVYWLAMPFFAPVPWEAEKFFFQPGMAEKNFTLDWWPVGTGAYMLTENNPNARMVL
                     EKNPNFRGEPYPSEGEQGDAAARLLGDAGKTMPFVERVVYSREKEAIPYWNKFLQGYY
                     DSSAIPSDTFDQAVRVSVGGEVGLTQEMQDRGIKLRTSADVTILYLGVNWLDDVVGGD
                     SVRARKLRHAIAIAVDWEEFISIFLNGRGKVAHDPLAPGIFGQRAGAEGINPLTHVWD
                     NGRAERRPIEEARRLLAEAGYPNGRDAKDGKPLVLYLDTVGRGPGDKPRFDWWRRQFA
                     KISVQLEIRDTDWNRFQEKIRKGSQQLYILGWNADYPDPENFMFLLHGAQSRAKSQGE
                     NTSNYVNPEFDRLFERMKTMPNTPERQAVIDRMVELYRRDAPWVGGFHPVSYGLTHAW
                     MMNGKPNNMARNNLKYLRVDIEQRAALRREWNQPVVWPLGLLLLALVVSAIPAVRSYR
                     RRERMAAKPA"
     gene            9789..10766
                     /locus_tag="AC16O9_07475"
     CDS             9789..10766
                     /locus_tag="AC16O9_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516493.1"
                     /translation="MLAYIIRRTLYAIPILVGINLITFALFFLVNTPDDMARMQLGVK
                     RVTPEAIQKWKADRGYDKPLLWNAGAPGAGRFTETIFFEKSVKLFAFDFGRAEDNRDI
                     GNEIRTRMGPSLALAIPVFLVGLAVNITFALGMAFFRASYLDLWGVVLCVAMMSISSL
                     FYIIGGQYLISKLWHLVPISGFADGTDALKFLVLPVAIGVIGGIGAGSRWYRTLFLEE
                     MGKDYVRTARAKGLAESVVMFRHVLKNAMIPILTGVVVVIPLLFMGSLIVESFFGIPG
                     LGSYTIDAIQAQDFAVVRAMVFIGSVLYIVGLLLTDISYTLVDPRVRLA"
     gene            10768..12120
                     /locus_tag="AC16O9_07480"
     CDS             10768..12120
                     /locus_tag="AC16O9_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516494.1"
                     /translation="MLVFLPTDVLIWLLVAAVATFAWYSSRRAHLAAPWARVFRSRIA
                     VACAVVLGCFIAVGLLDSLHYRPKLPQKGPQAPAAYSVQVNSALDALLTPLRTRLEKT
                     YSAPLATRLYQKESVERPGGITVREFPRLAHGGAHLEDESEWGRDVAVRVLAGLAGAA
                     LLWGLVVFAGRRTLARRWPAVPWGAAAAALGAVLALAGPAAVLAEGYHVLGTNQVGSD
                     VFYQALKSIRTSLVIGTLTTLVLLPLGIAAGIAAGYFRGWVDDAIQYVYTTLNSIPGV
                     LLIAASVLMVQVYVDTNPELFPTAAQRADFRLLALCAILGAVGWTGLARLLRGEALKL
                     SELEYIQAAHAFGVSHARILARHILPNVAHIVIITLVLDFSGLVLAEAVLSYVGVGVD
                     PSTISFGTMINAARMEMAREPMVWWSLAAAFVFMFALVLAANLFADAVRDGFDPRVRV
                     "
     gene            12117..13931
                     /locus_tag="AC16O9_07485"
     CDS             12117..13931
                     /locus_tag="AC16O9_07485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516495.1"
                     /translation="MSAVLEVKGLRTELDAPDGVVRAVDGVDLEIRKGECFALVGESG
                     CGKSMTALSILRLLPDVGRIAGGSVRLGEVDLLALPEAAMRAVRGRRAAMIFQEPATA
                     LNPVLTVGRQVAEVIERHTSLRGAAVRKRALELLHAVGIPEAERRVDEYAFQLSGGLR
                     QRVMIAGALAVDPEVLIADEPTTALDVTIQAQILELLSKLQRERGMALLLITHDLGIV
                     AQMADRVAVMYAGEIVEVAPREAFFRAPQHPYSQKLFAALPSAAQRAGPLAQIPGQVP
                     PLTGEFTGCRFTERCEFAFERCRREAPKLLQVDPAHRARCHLREKDRAAPRAAATGAP
                     EARAGGAARPLLQVQDLKVYFPIRRGVLRRTVGYVKAVDGVTLAVPEGRTLALVGESG
                     SGKTTVGKSILQLIRPTAGSVRYAGEELTGLGRAALKPHRARMQIVFQDPYASLNPRM
                     RVADILLEGMRALGVGAGEADRVKRCDAILEQVGLPAAAKSRYPHEFSGGQRQRIAIA
                     RALAVEPRLIVCDEPTSALDVSVQAQILNLLRELQERLGLAYLFITHNLAVVEYLAHE
                     VAVMTAGRIVEQGAAEQVLRAPRHAYTRALLESVPKID"
     gene            complement(13928..15394)
                     /locus_tag="AC16O9_07490"
     CDS             complement(13928..15394)
                     /locus_tag="AC16O9_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6516496.1"
                     /translation="MQGLRGFSGSIFVAAALLLQACALVPEPAPEAPPVSLAIPETVP
                     PADPAPLPVAKKPLPLPPLPPPPLPVVRLLDAERLPPFEGEKRELAEPQAIAKLDRTQ
                     RVDDLWQRLRRGFAMPDIRSPLVDERTRWYIANQDYLQRVFERSRRYLFHIVEALEKR
                     NMPTELALVPMFESAFNPMAYSRSHASGLWQFIPGTGRRYELKQNMWYDGRRDVVAST
                     DAALDYLRDLYQMHGDWHLALASYNWGENNVARAIAKNRKAGKPTDYLSLPMPRETRG
                     YVPKLQALKNLIANPAAHGIALAPIPNEPYFATVTKTRDIDVKLAAELAEMSVEDFVN
                     LNPGFSRPVIRSAVAPRIVLPADKVDVFHDNLQKLEDDQLVSWKTYKPKRGDSLESIA
                     RRPGLSVAQLREVNGIHPRSRRMPELLVVPIEGGESFARLPIMYAPPVPAGGRTHVVR
                     RGETPWSIARRYGIPLKDLQRWNRIGSHIRAGQRLTIF"
     gene            complement(15415..16179)
                     /gene="gloB"
                     /locus_tag="AC16O9_07495"
     CDS             complement(15415..16179)
                     /gene="gloB"
                     /locus_tag="AC16O9_07495"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR6516497.1"
                     /translation="MLEIVPLPALRDNYIWTLRDARHAAVVDPGEAHPVLEYLERERL
                     ELCAILATHHHPDHVGGIPELLALRKVPVYGPKGEPIATLTRPVREGDTVQIPELGVT
                     LSVLDIPGHTRAHIAYYGAGSLFCGDTLFACGCGRLFEGTPEQMMASLAKLAALPDAT
                     KVYCGHEYTLANIAFAQQVEPQNAALATRLARDRKLRDAGKPTLPSTLGEERATNPFL
                     RWAEPAVVESANKYLGSRVSDPVRVFAAIRDWKNRG"
     gene            16180..16962
                     /locus_tag="AC16O9_07500"
     CDS             16180..16962
                     /locus_tag="AC16O9_07500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516498.1"
                     /translation="MDRQSFVSEAAAKYNGERASFSGWCATPLGRYVLDWEMQQFDNA
                     VEDVFGYRAVQVGLPEFAFLRQNRIPYRFTVALEEGAALAADPLQLPLASQSLDLVVL
                     PHALESHLDAHSVLREAERVLRPDGQVVISGFNNASLWGLRRWFAGRNAGSPWDARFI
                     GLLRLKDWLQLLSFDLNGGRFGCYVPPLTQPAWRERFAFMEKVGARWWPILGGVYVVR
                     AVKRVHGMRLITPAWRKERKRRRALAAMPQKNGHVTQRRHDR"
     gene            16952..17392
                     /gene="rnhA"
                     /locus_tag="AC16O9_07505"
     CDS             16952..17392
                     /gene="rnhA"
                     /locus_tag="AC16O9_07505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR6516499.1"
                     /translation="MIAEAVEAFADGACRGNPGPGGWGVLLRVGGRERELHGGEAQTT
                     NNRMELTAVIRALEALAPGSSVRVYTDSQYVQKGITAWIHDWKRRGWRTADKKPVKNQ
                     DLWMRLDELARAHDVEWHWVRGHAGHPENERADALANRGIDSGV"
     gene            17404..18105
                     /gene="dnaQ"
                     /locus_tag="AC16O9_07510"
     CDS             17404..18105
                     /gene="dnaQ"
                     /locus_tag="AC16O9_07510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR6516500.1"
                     /translation="MSVPRQVVLDTETTGLSASLGDRVIEIGCIELLSRRVSDNHFHR
                     YVNPERKSDEGALRVHGLTEEFLADKPKFAEIAGAFLDYVRGAELIIHNADFDVEFLD
                     LELERAGLGRLKEHVAGIVDTLALARELHPGRRNSLDALCERYAVNNAHRTLHGAILD
                     ARLLAEVYLAMTRGQDSLAMDLETAAEQAAVTARIDASKLVVLRASEEEERAHEAYLD
                     GMAKEAGGKIVWRGA"
     gene            complement(18106..19206)
                     /gene="arsS"
                     /locus_tag="AC16O9_07515"
     CDS             complement(18106..19206)
                     /gene="arsS"
                     /locus_tag="AC16O9_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551656.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR6516501.1"
                     /translation="MRPASIPLRLEEIAVELADDRASPRAAQSLKRRQAPLAAPAEQL
                     KLLAGVPLRREFGAMAPDLAPAQLEIFQVNLGKLCNMTCRHCHVDAGPDRTDAVMDDA
                     TVDAVIAAVRATGAHTVDLTGGAPELHPRFRDVVQAAFDAGKHVMDRCNLTVLLLPRN
                     AGLVDWLAERNVEIVASLPHYRRPNTDAQRGDGVFERSLRALRILNAAGYGVGDPKRR
                     LTLVSNPAGAFLGAAQASVEREWREGLQRNFGLTFDRLFVLNNMPIARFLEWLQDTGN
                     LEAYLERLANAFNPGALAGVMCRSTLSVGWDGRLYDCDFNQMLELALDAAAGAHIRDF
                     DERAWRRRAIVTARHCFGCTAGAGSSCGGQTA"
     gene            complement(19209..19541)
                     /locus_tag="AC16O9_07520"
     CDS             complement(19209..19541)
                     /locus_tag="AC16O9_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HIR6516502.1"
                     /translation="MNHYFDPQHLADFGKVSEASPELGKKFFDYYGAVFADGALSARE
                     KSLIALAVAHTVQCPYCIDAYTQDCQQKGADLEQMTEALHVAVAIRGGSSLVHGMQMR
                     EQAKKRGM"
     gene            complement(19593..19931)
                     /locus_tag="AC16O9_07525"
     CDS             complement(19593..19931)
                     /locus_tag="AC16O9_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516503.1"
                     /translation="MAAWFLPALKAVLPHVGTLITVAAPVFTKKSAEAANQAQLMQQQ
                     IAELQAAASQNADNIKALAEQLQGTVSALHQAAAIAEARLRRAYLLCLAAIALSAATL
                     GVALFVIFAR"
     gene            complement(19950..21908)
                     /locus_tag="AC16O9_07530"
     CDS             complement(19950..21908)
                     /locus_tag="AC16O9_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6516504.1"
                     /translation="MTLSDGIAWRPDPQGVAQANATAFMRALGVAGYEELLACAEREP
                     ERFYRALIGAAGFAFYTPYTRALDESAGIAWPRWCVGGTTNVVLNALDRWRGTPTYGK
                     TALVWEGEDGARRSLTYRELDGEVCALATGLRALGLGRGDVIAMYLPSLVETVVAALA
                     IPKIGAIVMPLFSGFGADAMADRLALGGARALVTVDGSLRRGKLVDAKAVVDEAARGA
                     PALEHVIVLRHARNAVAWDARRDHWWHELAAGRGAGAPTEPMEADAPYLLLFTSGTTG
                     KPKGVVHTHVGFPMKVLLDMWLTMDWKSSDRILWMSDMGWVVGPMLAYGTPVMGGTLV
                     AVEGAPNFPEPDRMWRAAAEHGVTYLGVAPTTIRTFMAEGCEPWRRHDLSRLRVMISS
                     GEAWTPDAWHWLFERVGGGRVPLLNFSGGTEMIGILGCTPLRPLKPCSFNTPIPGAGA
                     DVVDERGAPVPPGTVGELVMRRPPIGLTRGLWQDDARYIESYWSTWPGVWHHGDFAVR
                     DADGYWYIRGRSDDTLKIAGKRTGPAEIEALLMATGKLAEAAAIGVPDPIKGQALVLA
                     CALKPGVAPAASLQSELEAVVTRGLGTPFRPKAVLFVSELPKTRNLKIMRRVIRAAIL
                     GENPGDLSALVNPQVIDELRAARGGAAG"
     gene            complement(21905..22177)
                     /locus_tag="AC16O9_07535"
     CDS             complement(21905..22177)
                     /locus_tag="AC16O9_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516505.1"
                     /translation="MKLNITALALTAGLFWGAALLIVALANLASPGYGSAFLELSASI
                     YPGYRPGTGAGSVIVGTLYAAVDGAIAGAIFGWLYNLLARAFPAQA"
     gene            complement(22233..22883)
                     /locus_tag="AC16O9_07540"
     CDS             complement(22233..22883)
                     /locus_tag="AC16O9_07540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516506.1"
                     /translation="MSTFVSGQEKSLGLYSRFILPRAVNFACGLKPAMRQRQKVVPLA
                     RGRVLEVGIGTGLNLSFYDAAKVSKVWGLDPAPEMTRMAKRAAGARPFEVEFIEAPSD
                     AIPLDENSVDTVVVTYTLCTIPDTTAALRQISRVLRPGGELVFCEHGAAPDASVRRWQ
                     DRLNPLWKRLGGGCNLNRPIPALIETAGFRITSLDTMYLPGWRPASFNYWGTAVPR"
     gene            23038..24678
                     /locus_tag="AC16O9_07545"
     CDS             23038..24678
                     /locus_tag="AC16O9_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6516507.1"
                     /translation="MQKSSAYEQDLYRNPANYAPLTPLAFIERAASVYPRRTAVIHGA
                     RRFTWGETYARCRRLASALAKRGIGKGDTVAAMLANTPEMYECHFGVPMTGGVLNTLN
                     TRLDTDAIAFMLNHGEAKVLITDKEFAPTIEGALKKLGRAIEVIDVEDPEFEGGKRLG
                     KATYEQLLEEGDPAFDWRWPEDEWQAISLSYTSGTTGDPKGVVYHHRGAYLNAVCNIL
                     TWGMPHHAVYLWTLPMFHCNGWTFPWTMAANAGTNVCLRRVEAAKIFELIKAHKVSHY
                     CGAPIVHSTLVNAPEAMKKGITHKVSAMVAAAAPPAAMIEGMEKMGFDLTHVYGLTEV
                     YGPATVCAKHEEWGAADISERARLNGRQGVRYLMQEGLSVMDPQTMKPVPADGEAMGE
                     IMFRGNITMKGYLKNPKATQAAFAGGWFHSGDLAVMQPDGYVKIKDRSKDIIISGGEN
                     ISSIEVEDVLYRHPSVLAAAVVARPDEKWGETPCAFVELKPEAKASETELIEFCRQHL
                     ARFKAPRAVVFGELPKTSTGKIQKFVLREKAKSASAIE"
     gene            24689..25486
                     /locus_tag="AC16O9_07550"
     CDS             24689..25486
                     /locus_tag="AC16O9_07550"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6516508.1"
                     /translation="MSAVPQAEQPVLLREDLNGVTTLTLNRPQQMNLLTREMIEALQA
                     AFDSIAKDPDVRVVVIAAAGRGFCAGHDLKEIRALEELPKIEGLFFACSRMMLTIPML
                     PQPVIARVQGAAAAAGCQLVAQCDLAVASEAAKFTTPGVTWGFYCSTPGVAVGRNLQR
                     KHALEMLLTGDLVDAKRALEWGLVNRVVPAHQLEAETGKLARQLADKPPATVAAGKRA
                     FYQQMEMGTEKAYELASHVISSSFAHPEGREGMDAFIEKRSPPGQKT"
     gene            25488..25931
                     /locus_tag="AC16O9_07555"
     CDS             25488..25931
                     /locus_tag="AC16O9_07555"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6516509.1"
                     /translation="MGRYYDDFAVGDTYKHWPGRTISAADNTWFTLLTMNQHPIHFDE
                     EYAKSSEFGRPLVNSALTLAIVVGMSVRDVSQNAIANLGWTDIVMPAPVFNGDTLYAE
                     SSVLEKRESKSRPGQGIVTVTTRGWKQDGTTVMTFKRSILVPKKP"
     gene            25928..27067
                     /locus_tag="AC16O9_07560"
     CDS             25928..27067
                     /locus_tag="AC16O9_07560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6516510.1"
                     /translation="MSEDTQILDAIGKWLERDVRPKARELEKKDEYPEKMVEQMKELG
                     LFGATIPEQYGGLGLSASTYAKIVAQISEVWMSLTGIFNSHLIMAAAVARYGTDAQKQ
                     TFLPKFASGEVRGGLALTEPDCGTDLQAIRTRAVKQGDHYVINGTKTWISNGIKGSCF
                     ALLVKTDPNASPRHKGMTMFLAEKGKGFRVSKKLEKLGYKGIDSAELIFEDYRVPAAN
                     LIGGAEGKGFHHAIGGLELGRINVAARGVGVANAALKDAVAYSQVRKTFGKPICEHQA
                     IQLKLGEMATRCEAARLLVENAARAYDAGERCDMEAGMAKYFASEAALENSIEAVRIH
                     GGYGYSTEFNVERYYRDAPLMCIGEGTNEMQRIIIAKQLIARHPA"
     gene            27064..28242
                     /locus_tag="AC16O9_07565"
     CDS             27064..28242
                     /locus_tag="AC16O9_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6516511.1"
                     /translation="MMLPLAGLRVIAVEVYGAGPFGSTHLADLGAEVIKIEPREGGDT
                     SRAVGPFMLGEDDSVFFQSLNRNKKSLTLDLKHPKGKEILAKLAATAHGLMSNLRGDQ
                     PEKLGLTHAALARANPKLVCAHLSAYGRDGARKSWPGYDYLMQGEAGFLGLSGEPDGP
                     PARMGLSIVDYSTGTMCALALLAGILEARKTGRGRDLDVSLYDVAMHLLNYPAAWYLN
                     EGLVTERVARSAHPFIVPSQLYRTKDGWIFVMAQNQRFWERLADALGRRDLKAKYPDF
                     AARREHRDELTQVLDAEFAKQTTESWLAKLRGTIPCGPVYDLPQALNNPYFVERGGVQ
                     VFDHPDKPGFKLVASPFRIGEPLPARPAPKLGEHTEALLKELGYADAEIARLKAARAV
                     "
     gene            28242..29411
                     /locus_tag="AC16O9_07570"
     CDS             28242..29411
                     /locus_tag="AC16O9_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6516512.1"
                     /translation="MKLEGIRVIDLSLFLPGPLATQFMADHGAEVIKVESIHEGEPNR
                     EIGAKRGGTSVYFANTHRGKQSLALNLKDPAGVEVLLRLAEKSDVLVEAFRPGVVDRL
                     GIGYAQVAKRAPQLVYASIAAFGQRGPYRDIAAHDLATEAMTGVLSLNRGGDGKPAIP
                     ALANADMLASMMTLSGILMALLRRKDTGRGDYLDMAMADALLACLPNNMGVAMTERRQ
                     PDLKGERALGGNALYNIYETKDGDYVVLAGQEPKFAKNLLEPLGRADLVELCKLPPGP
                     GQDPVRAFLRETFRTKTKAEWVEWFKGRDVAFAPLRTLPEVLEDEHFKARGMVLRDDR
                     GWDHLGIPIAFREEPGRVRFDAPAHGTHSRAILRGLGYGAAEIAQLESRGVIKSA"
     gene            29408..30289
                     /locus_tag="AC16O9_07575"
     CDS             29408..30289
                     /locus_tag="AC16O9_07575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6516513.1"
                     /translation="MSRHLVILNPVAGRGRARREWPRMADALRAAGVAFEVAQTSTPG
                     EAERLAAQGASRFDALIAAGGDGTVHGVVNGLLGAGGTAALGVLPLGSGDDFAKMLPA
                     GDPIARIARGAARAFDAGRIAAGATRFFANGMDVGFGAHASRNVRRVPRPFTGLGAYL
                     GALFLTLVRYPRLELRLQLDEGEAFAQTTAMTAVMNGRAFGGSFHVCPDARADDGELD
                     LLIAEGVGRLAILGLVPRIMRGTHGGDPRLTLTRARKVLIESEAPLLVEADGEIVFED
                     ARRLEIAILPGALRVLA"
     gene            30392..31180
                     /locus_tag="AC16O9_07580"
     CDS             30392..31180
                     /locus_tag="AC16O9_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6516514.1"
                     /translation="MLRSLVIWFIVLAAIAAWVPLVAVVRVFDRHPARLRTGRWFRRL
                     GVVVTRLNPLWKLRVDGIPAGGLRAPCVVVSNHQSLADIPLLSQLPWEMKWVAKRSLF
                     RLPAIGWMMRMAGDIPLQRTDPRSGARSLLHAARYLRQGCPVMFFPEGTRSRDGGVGR
                     FHDGAFRLALRERVNVLPIAIDGSHACLPKHSWQFGAARTIRVRVLPAIEIAGFGPDQ
                     TEALRDAARRAIIRQIALWRDADESAVDAAGKAAAAPFAAPSRV"
     gene            31183..32355
                     /locus_tag="AC16O9_07585"
     CDS             31183..32355
                     /locus_tag="AC16O9_07585"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6516515.1"
                     /translation="MHPPPPLPHLAIRGKRLLPIVQGGMGVGVSAHRLAGSVASLDAV
                     GTIASVDLRRLHPDLMERTGRSRDKSAINAANLEALDREVRSALGLAQGRGLVAVNVM
                     RAVSQYADYVRQACESGIQAVVMGAGLPLDLPELTAGFGDVALIPILSDARGTTLLLR
                     KWMRKQRLPDAIVLEHPRYAGGHLGAADEADVLSPRFDFATVIPAVLASLKELGLERE
                     NIPLIAAGGINSHEKVRAVLGLGASAAQLGTAFAVTEEGDAHPVFKKVLADARPEDIV
                     TFMSVAGLPARAVRTPWLDRYLRREERMKGKARPKPQCTLAWDCLLHCGLRDGLARAG
                     QFCIDNQLGAALRGDLERGLFFRGSEPLPFGSAIRSAADLIGYLMSGVYPPGVQPR"
     gene            32404..32712
                     /locus_tag="AC16O9_07590"
     CDS             32404..32712
                     /locus_tag="AC16O9_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516516.1"
                     /translation="MNRPCVVRIVALLLVLVGLQAGAFRDLDTGHEWYLGAEDNSAAV
                     MAESREADRPITGVPAAHAPSALPAPVGAALEIDAAPGSAGPSAPRAFRPHATGPPRA
                     "
     gene            32790..33419
                     /locus_tag="AC16O9_07595"
     CDS             32790..33419
                     /locus_tag="AC16O9_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6516517.1"
                     /translation="MDTLQQQAGARHAVLHRLIFGIDFLSRTVGHSFAWCIAILTLGT
                     CFEVMVRYALNDPTSWAFDMSYLMYAGVFYMAGAYTLSRAAHVRADMLYRLWRPRVQA
                     AVEFTLYVFFFFPGILALVIAGWGYGTESTRILESSVNSPAGVPIWPLKMMIPLGAGL
                     LALQGLAELLRSWVCLREGEWPPRLHDVEELEKQILMKAADMREKGGAL"
     gene            33416..34759
                     /locus_tag="AC16O9_07600"
     CDS             33416..34759
                     /locus_tag="AC16O9_07600"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6516518.1"
                     /translation="MSDPVVALVMLGILVSTIMIGFPVAFTLMALGVGFGYYAYFQPG
                     QAFFDNRVFYLLTQNTFTVMNNDALVSIPLFLLMGYMVERANILDSLFGSLQVALRAV
                     PGSLAVATLATCALFATATGIVGAVVTLMGLLAFPQMLKAGYDTRLAAGVVCAGGCLG
                     ILIPPSIMLIVYGAQASISVVKLYAAALLPGFLLAGLYIVYVIGLAVFKPAAAPALPK
                     ERTDIAFGQVLLMLAKSFFPLAILILSVLGAIMFGIATPTEAAAVGAFGAVVLAAGYR
                     SLTWQGMREAVYLTARTSAMVCWLFVGSATFASVFAYLGGEGLIKSTVLGMNLSPISF
                     LLLSQFIIFLLGWPLEWTEIIIIFVPIFLPMLPHFQIDPLLFGVLVALNLQTAFLSPP
                     MAMSAYYLKGVAPKHVLLTQIFGGCMPFLYIVIVCMAIVYIFPDVALWLPRALYG"
     gene            34785..35774
                     /locus_tag="AC16O9_07605"
     CDS             34785..35774
                     /locus_tag="AC16O9_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR6516519.1"
                     /translation="METLSTAVLVFVVSAAFVIAAGIGLARYGDDLAESTGWGKLWVG
                     TLLVGIATSLPEVTVNISAVWLEDSPGLALGNVFGADMMNVFVLGCVGLAFGIGNLFG
                     GQGRDTELLILVGMALVALALVFGIAGDVKLGPTSLGTALILAAYLVGMRMVYKAGQT
                     HMHLEDIPAPTGSAKKAWIGFGISVAVVIVAGRFLAASADRIAEISGISASFIGVLLV
                     SIVTTLPEGSVTVAATLRKSYGIAMGNVYGSNAFNVTILTFSDLFSPAPLLGKMEGAH
                     FAAGLAAFVLMLIGYLVWKGVQRPAFAWARTLAPAIPVVYVGALYIVYTLAQA"
     gene            35823..36863
                     /locus_tag="AC16O9_07610"
     CDS             35823..36863
                     /locus_tag="AC16O9_07610"
                     /inference="COORDINATES: protein motif:HMM:NF023696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HIR6516520.1"
                     /translation="MLAVWRLASAALGLAAAALVQAQGSDELWNMSVRMEMDGMQMPA
                     MSQQVCQKKGQPGAAPAPDPNCTTSDVRTVGNKTTWKMACTGDEPMSGTGEITRTRDS
                     MSGRMQLRGKDGLTMNVVYSGTLAGTCNAQTHQDPQQAATKQKMAAMQAQSNAQLAQT
                     CRESIEKFATTLFEMQGNPCAASTGEYCAHVKKTAQSMRTPAGYRQAMQVEGLRNDGF
                     ERAAQFCKVDPAPVRAAACKSAVDGRDWPFVTEYCPAESQTLAKQHCAGREYTVVMKT
                     EYGPLCSKHSNLLAKGPCGGGRSYTVVKAGGGWDTKCGERPAQVATPPAAPSAGDAVK
                     EGVRGLRKLFGN"
     gene            36869..>37521
                     /locus_tag="AC16O9_07615"
     CDS             36869..>37521
                     /locus_tag="AC16O9_07615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR6516521.1"
                     /translation="MAWRLALAALLALAGPVAHAAPEDPFPRIAAAYLVLVDGEPLWA
                     HAPDARRAPASLAKLMSALVVVEQDALDEVVTIGAEAARARGSRLGLHAGERVPAHGL
                     LAAMLVGSANDACYALAARRGRVSDFVARMNARAAELGLRATRFGDPCGFDRDRQYST
                     ARDLARLAQAFMQRAQLAALVGQLRVEVATEGRGRVFTVKNTNVLVEFFPGTIGVKTG
                     "
BASE COUNT         5652 a        12922 c        13454 g         5493 t
ORIGIN      
        1 ccttcggcga gctgcgccgc ggcaaggcgc gccatctcgc ccgtcggatc gcgggcgagc
       61 agcgcctgcg ccagcgcgcc ggcggaggac gccaccaggt cgagcgtgtc gccgatcgcc
      121 tcgcgcaatc ccgccgcgct gaagcgctcc ggcgtcaccg ccgggctcag caggtaacgg
      181 tgctcgaaga ggatcgtcct ctcgcgctcg ctgccctgca tcgcgccgtt gccgacgtag
      241 gcgaagcgcg cgtcggcgcg caggcgctcg gcaagctcgc gcgaaagccg ggcgcgcgcg
      301 gcgctgtcgg cgccctcgat cgccatcagc atcacgcggc ccacggcgcc ctcgcgcagc
      361 tggtcgacga gcagctgctg cgccggcgtg ggcgcgcgcg gcaggaacgc ggacaggtcg
      421 gcggtatagc gcgcggtcgc ggcgagcgcg ccggcgacgg ccagcccggc cagccacgcg
      481 gcgagcggcc cagcctggcg cagcctcatg gcgcgcccag ccgctgcacg ctcatcagcg
      541 tgctgtcgcc gttcgcctgc gcgatctcga tcacggtcac ctccgccccc tgtccggcga
      601 tgcggatgcg cgccaccagg ttcgccatgc cggtgtcgcg cggcgtgagc gtgagcgccc
      661 aggagcgctc gctgccctcg aggcgcacgg cgtagatgcg ctccagggcc tcgcggtcgc
      721 cagcgagggt ggcgcggatg ctctcaacga aggcacctgc ctcggggtgg ctgcgcagat
      781 cgagctgcag ccggcgctcg ccgcgctcca gcgtcagcgt gtcgccgtgg agccgcagta
      841 cctcggggcg aggccgcagc gtgcgctttt ccagccggtc gggcgcttca aaacgcagct
      901 cgcccgagct gacgagcggc tgctcgagca cgcgcaccgt ctttttctcg acgaagctcg
      961 cgcgcgcctg cgctctctgc gcgaagccct gcagcagctg ctccagcgtc caaacgggcg
     1021 ccggccaaac gccaggcgtc gccaacagca gcagcgcgaa aaggctaagc cgcatcggcc
     1081 cagaaatcgt agaaattgaa ccagttgtac ggcgcagcgc ggcagtggcg ctcgaggcag
     1141 gcgacgtagg cggcgagcgc cgcctcgggc gccggggcgg cagcgccgaa gtccgccagc
     1201 ggctcgaact gcagcacata gcggttgccg ccgcggtaca ggccggccat gaacgtcacc
     1261 cggcagcgca gcagcgccgc catgcgcatg gggccgagcg gaaagcgcgc cggcgcgccg
     1321 aggaacggca gcgtgcacgt gtcgtcgtcg tgcagcgcgc ggtcggcgag caccccggcc
     1381 atgccgcccg cggcaaggca gtcgcgcacg cgcagcatcg agtccacttg ccctagcgcg
     1441 attacatcct ggcgcgctgc cgggctgagc gcggccagcg tcgcgttcag catgcgcgca
     1501 ttgtcctcgt acatcaccat cgacacctgc aggccttggc gttggcgtcc ggcggcgcgc
     1561 aacgcctcga agctgcccag gtgcgcgccc atcagcagct cgcccgcgcc gggcgagggc
     1621 gggtcgcgca cctcgatgtc gaacagatcg aagcgcccgt tcagcaggta gacgcggtcg
     1681 agcacggtgg cggcgaaggc gtagaagtgc cggtacagct cggcgagcgt cgcgcggcgg
     1741 ccgagcgagc gcgacaggta cgcgcgcgag gcgcggcgcg cgcgcggcac cgcgagaagg
     1801 aaatacgcgc tcactgggtg cagcacgagg cgcgcggcgc gccggcccag gccgagcgca
     1861 atccagcgca tcgcgcgcag cgccagcgga tggctgcgct cgggcgtgcg gtgccagggt
     1921 gcggtcacgc ggcggcggtc ttgagctgcg cggtggcgac cacgcggtcg ccggcacgca
     1981 gctcgatgcg gtttccggtg cggcgcacct cgacttcgat ttcgggcggc accgggtgca
     2041 ggaacttggc cgaggcgatc tcgagccccg ggcgcgcgag gcccagcgcg tcgagcgcca
     2101 cggcaagcag cagcgcgccg ggcaccatcg gcgcccccgg gaaatggccg gcgaacgccg
     2161 ggtgcgcggc ggaaaagcgc aggcgcgcct cgctcacggc gcgcgctcca cgccgtgcga
     2221 ccgcgccagc gcgcgcagcg cttcgcgcgg caacttgcca ctcgccgcgc gcggcaggcg
     2281 ctcgagcagc accagggggc gcggcaggaa ggccgcgtcg atgcgctggc gcagcgcggc
     2341 cagcagcgtt tcccgcgagt gtccgggggc gaccacgaat gcggcgaggc gcgtcacgcc
     2401 gcctgccgcg ccatcgtcgg gcatgtagaa cacgccgtcg gtgacgccgt cgatcgcgcg
     2461 cagctgcgcg tcgaggtgcg cgagcgagga gcgcttgccg gcgacgttca ccatgtcggc
     2521 gaggcgcccg cgaagcagga accggtccgg cgccagcggc tcaagcacgt cgccgagcgc
     2581 agtgggctgc tcgacgtggc cgccgctcgc ccaggccact gcgtcgcgga gctcgacgcg
     2641 aatgccgggc agggtgcgcc attcgagctc gcgcgccgtg cgccgcgtgg ccatctggcc
     2701 ggtctcggtg cagccgtaga tttcgaggag cggcgcgccg aagcgcgcct cggcgagcgc
     2761 ggcgagctcg gcctcgagca gggcggtcgc gcacagcagc aggtctgcgg cgggcggttg
     2821 cgccaccgcg tcgagcagcg tgcgcagatg aaacggcgcc gtcaccagca cgcgcggccg
     2881 cggcagccgc gccagcgcgg cgcagatctc ttccggatca aacgggcgct ccgcgcacag
     2941 cgcgccgccg ccggccagcg cggcgagcac cgtggactcg aagccgtaca tgtgctgcgg
     3001 tggcacggtg ccgagaatcg cggggcgcgc gcccgccagc gcccagcgct cgagcgcgcc
     3061 ggcgacgtta cgcgataacg tgccccagtg cttgcggttg gggaccgggg tgccggtgga
     3121 gcccgaggtg aagacgatgg cacagagctg atcgtgtgct atttcgggca ccgcgaacgt
     3181 cggcgggagc ggggccggct ctgcgggcgc gggcacgcac ggcagatcga tctgcacagt
     3241 cgcatcgtcg gcgagcaggt aggcgtcggc ggcaaattcg cgcaggcgct gcaccgtctg
     3301 cggcgtgtga tcgggcggca gcagcgtgac ctggccgcgc agcagcgcgg cggcgaggcc
     3361 ggtggcgaac cgatagcggt cggtacaggc gttgagcacg tggcggcgcg cgggcaactg
     3421 cccggcgagg cgcgtggcgt cggcgacgaa ctgcgcggcg ctcagcgcgc ggccgtcgcg
     3481 gtaggcgacg gcgctaccgg ccgccgcgcc ggcaatgagc ggaaccgcgg tcatgctcag
     3541 tgcgccgcgc ggctagcgcg gcccggcggt ggtgcgcgcg cccgaatccc agtacgcgcg
     3601 cacgctgcgc gtcacgccgc ggagcacgcc gccgtggctt agctccgggc agacgcgcac
     3661 gcgcaccagg aactcggcga cgaacatcgc gccgaccagc ggcagcgtga gcagattggc
     3721 gaacaccgac cacagggcaa gcggcgcgag gaaaaacagc agcgccgagg cggtcgcgat
     3781 ggcggcgctg aatacggccc aggccagggt cgcgccgcgc gtgtaggcga ggcctgccgg
     3841 gctgagcgtg ccttcgacca gcagcgcgaa gcgcgtcacc agcgcctcgc gcccggggcg
     3901 caggctggca ccgaaccacg ccgccagcgc gagaaacatg gccacgtgct gcaggaagta
     3961 gaaccacgga acgtaggggc cgaggccgcc gagcgccgcg cggccgggtg cggtcgtcag
     4021 ccagtgcgcc agcacggcgt agcccagcgc gagcgcgatg agcagcgcga gccagccgcg
     4081 tgtgcgcgcc cggctcactt cgtgcggttc tgcgcgatga agcgggcgag gttggagagc
     4141 gacgcgaaga tcaccgtgtt gtcctcgtga tcggagcgca gccgcacccc atagcgccgc
     4201 gacacgacca gggccagctc gagaatgtcg atcgagtcga ggccaaggcc ctcgccgtag
     4261 aggggcgcct ccgccgcgat gtcgctgggc gcgacgtcca ggttgagctc ggccgtgatc
     4321 agccccgcca gctcctcgag gagcgccggg tcggcggcgg gggcgaactg cgtgctgacg
     4381 ggaatgctgg ccatggtcgc gggaaacagt gtaacggctg cccgggcccc gttcaaaccg
     4441 gctgtggcgc gcggaacgcg agcaccgcgt tcgtgccgcc gaacgcgaag gcgttggtga
     4501 gcgcggcgcg cagcggcagg tcgcgccgcg ccgcgcccgt gacgtgcagg acgcccgcgc
     4561 actccgggtc gaggtcggca gcgtcgccga gcgtgggcgg gatcgcgccg ttgcgcaggc
     4621 cgaggaccgt gaacgcggcg gcgagcgcgc ccgccgcgcc gagggtgtgg ccgtgcgccg
     4681 acttggtggc gctcaccgcc acctcggcag cgcgcccgcc gagcgccgcg gcgagcgcgg
     4741 cgacctcgat ggcgtcgcct tcgcgcgttc cagtggcgtg cgcgttgaca tagtcgacct
     4801 ccagcggcgt catcgcggca tcggcgagcg ctgcgcgcag cgctcgcgcc tggccttcgc
     4861 tcgacgggcg taccaggttg ccgtggtcgc aggactcgcc gtagcccgag atctcggcgt
     4921 agatgcgcgc gccgcgccgc tcggcgcgcg cccagtcttc gagcaccagc gcggcgccgc
     4981 cctcggcgag caccagtccg gcgcgctcgc gctggaacgg cttgctcgcg agtggggcgc
     5041 tctcggcgtc gcccggcgcg agcacctgca gcgcgagcca ggcgcggatc acgccgtagg
     5101 cgagcggcgc ctccgagccc ccggccagca tgacgggcgc ggtgccgcta cgcacgcgtc
     5161 gaaacgcgtc gccgatcgcc atcgccccgg aggcgcaggc gaccgaatag gtggcgcagc
     5221 acccgcccag ggcgagctgg atcgccaggt gcgaggcgca ggcgttgttc atcgcctgca
     5281 ccacggtgag cggcggcagg cgctcgcggc cgctgatgaa cagatcccgg tagccgcgct
     5341 cgaacaccat ggcgccgccc acgcccgtgc cccaggcgat gccccacgat tcgcggtctt
     5401 cgcttgccgc gggctcgaga cccgcgtcgc gccaggcttc gaggccggcg gcgatgccga
     5461 gctggctgta gcgatcgagc gtgccggccc gggcgcgccc gaacgccgcg acagcgtcga
     5521 aatccgcgca cacgccggcg ggcagcgaca gcggcttggg cgcatcggcg ggcgcgtgca
     5581 accgcaggcc ggagcggccc gcgagcagcg cctcgaacga cgcctgcgcc gtggcgccga
     5641 gcgggctgac catgcccacg ccggtcaccg cgacgcggcg cgcggtcacg atttcgcagc
     5701 cgccaggcgt tcgatcagcg ccaccagttc gccgcaactc ttcggcggcg gcgtgtcctc
     5761 gtccaccttg acgccgaagc gctcttcgat ctcgaacacc atctcgagca gcgacagcga
     5821 atcgaccccg aggctggcga gctcggtggc ggcgtcggcc gacttgacgt cgacgccgag
     5881 gcgctcctgg atgtagcggt aggcgggtgc gggatctgcc atgggactac ttgcccgcca
     5941 cggcgccgat cttcaggacc gccgcgcgca ggcgcttggc gtcctcgaac cagaagtcct
     6001 tgaactcggg agcgtcgagg tagttgatcg gcgtgcgcac cttctccatc gcgccggaaa
     6061 atagcggcgt cgccaccgcc tcgcgcaccg cggcgcgaag cttgtgctgc gcctcggcgg
     6121 gcgtcgccgc gggcgccacc acgccggccc agatgtagaa ctcgacgtcc tggtagccaa
     6181 gctccttgaa ggtcggcaac gccggcatca gctcgagccg cttggcgccc cagccggcga
     6241 gcgcgcgcat cttgcccgcc tggatctggc cgatcgcggc cgccggcccc gaggcgagcg
     6301 cctgcacctc gcccgagagc agcgcccgca ccgccggccc gccgccctgg tagggcacgt
     6361 ggaacagctt gatgcccgcg gcgttggcga acatctccat cgccacgtgc agggtgccgt
     6421 agatgcccga ggaggagtag ttgatcttgc cgggattcgc tttggcggcg gcgacgaaat
     6481 ccgccaccgt cttgtgcggg ctgtcgccgc cgaccaccag caccgtcgga tccgcggtca
     6541 ccagcgcgat cggcacgaaa tccgagatct ggtagggcgc gggcttgccc tgcagcgggt
     6601 ccgacaccgg gaagatcgag atcgaggaca gcgccatcag gatcgtgtag ccgtcggggt
     6661 ccgccttggc ggcggcggtc atgccgatgg cgccgccggc gccggtccgg ttgacgacca
     6721 ccacgggctg gccgagcgac ttctccatgg cggcggcgag cggccggccg acgatgtcgg
     6781 cgacgccccc cgggggaaac ggcacgatca tggtgatcgg ccgggttgga tactgcgcgt
     6841 gcgcgccgaa agcgagcacc gcgaacaacg ccgcaaagat tctcttcatg attcgctccc
     6901 tcctcctggt atgcctgcaa ccacgtttct gaatccggaa tccacgtaca gtacctcgcc
     6961 cgtgaccccg gcggcgagat cggacaacag gaataccgcc gcgttgccca cgtcctcggt
     7021 agtgacgttg cgccgcaggg gcgcgtgctc cgcgacaaac ttgagcaacc tgcccatgcc
     7081 ggcgatgccc gcggcggcca gtgtcttgat cgggccggcg gagatgccgt tcacgcggat
     7141 gccgcgcggg ccgaggttcg cggcgaggta gcgcaccgaa gcctcgaggc tcgccttggc
     7201 caggcccatg acgttgtaat ggggcaccac gcgtgcggcg ccaaggtagc tgagcgtgac
     7261 gagcgcaccc ttgccgccct tcatcatcgg cagcgccgcc ttggcgagcg ccgggaagct
     7321 gtaggccgag atgtcgaggg cggtgcggaa ggcctcgcgc gagagcccgt cgagaagttc
     7381 gccgtcgagc gtttcgcgcg gcgcgtaggc gatggcatgc accaggccgt cgagcgagtc
     7441 ccagcgcttg ccgagctggg cgaacagcgc ttcgatctgc gcgtcctcgc tcacgtcgca
     7501 gggcagcgcg atgtccgagc cgaactcgtg cgccatctcc tcgacgcgct gcttgaagcg
     7561 ctcgttctgg taggtgaagg caagggtcgc gccctcgcgc cgcgcggcct tggccacgcc
     7621 gtaggcgatg gagcggttgc tgatcaggcc ggtgatgagg atccggcgac cgtcgagtag
     7681 cgtcatggtg cgccgattat acttgcgcga tgcgcccgat cctgctgctg gcgtgcctgg
     7741 ccgctgccgg ctgcggcgac gtctggaacg atccctatcc ggcgtcggac cgcggcaaga
     7801 acatcctgta ttcggcgttc accgagcggc ccaagcacct cgatccggtg cagtcctaca
     7861 ccgaggacga ggcgcgcttc acccagcagg tgtacgagcc gccgctgcag taccactacc
     7921 tgaagcgccc ctacgagctg gtgccgctca ccgcgctggc agtgccgaag gcggcgccgg
     7981 cgccgggcgg cggcgtcgtc tacacgatcc gcatccggcg cggcatccgc taccagccgc
     8041 acccggcgtt cgtcgaggcg aaccaggcgc tcgcgcccga gcgcatcgcg cgcctggatt
     8101 cgccctaccg gctgccgctc ggcacgcgcg agctcaccgc ggatgactac atctatgaga
     8161 tcaagcgcct cgccagcccg cgcctgcact cgccgatcct cggcctgatg gccgagaaga
     8221 tcgtggggct cgccgagttc gccgagcggc tgaagaagtt cgacacgcgc aagcaggact
     8281 ggctcgattt gcgcaagcac cgtctcgaag gcgtggagaa ggtcgatgac catacctacc
     8341 gcgtgcggct gaaggagccc tatccgcagt tcgtgtactg gctggcgatg ccgttcttcg
     8401 cgccggtgcc ctgggaggcg gagaagttct tcttccagcc gggcatggcg gagaagaact
     8461 tcaccctcga ctggtggccg gtgggcaccg gcgcctacat gctcaccgag aacaacccga
     8521 acgcgcggat ggtgctggag aagaacccga actttcgcgg cgagccgtac ccctccgaag
     8581 gcgagcaggg cgatgccgcg gcgaggttgc tgggagatgc cggcaagacg atgccgttcg
     8641 tcgagcgcgt ggtgtactcg cgcgagaagg aggccatccc gtactggaac aagttcctgc
     8701 agggctacta cgactcctcg gcgatcccga gcgacacctt cgaccaggcg gtgcgcgtct
     8761 cggtgggcgg cgaggtggga ctgacgcagg agatgcagga ccggggcatc aagctgcgca
     8821 cctcggccga cgtgacgatc ctctacctcg gcgtgaactg gctcgacgac gtggtcggcg
     8881 gcgactcggt gcgcgcgagg aagctccgcc acgccattgc catcgccgtg gactgggagg
     8941 aattcatttc catcttcctc aacggccgcg gcaaggtggc gcacgatccg ctcgcgccgg
     9001 gcatcttcgg ccagcgcgcc ggcgccgagg gcatcaaccc gctgacgcac gtatgggaca
     9061 acggccgcgc cgagcgccgt ccgatcgagg aggcgcgccg gctcctggcg gaggcgggct
     9121 accccaacgg acgcgacgcg aaggacggca agccgctcgt gctctacctc gacaccgtgg
     9181 ggcgggggcc gggcgacaag ccgcgcttcg actggtggcg gcgccagttc gcgaagatct
     9241 ccgtgcagct cgagatccgc gacaccgact ggaaccgctt ccaggagaag atccgcaaag
     9301 ggagccagca gctctacatc ctgggctgga acgccgacta cccggacccc gagaacttca
     9361 tgttcctgct gcatggcgcg caatcacgcg ccaagagcca gggcgagaac acttccaatt
     9421 acgtcaaccc ggagttcgac cgcctgttcg agcgcatgaa gaccatgccg aacacgcctg
     9481 agcgccaggc ggtgatcgac cgcatggtcg agctctaccg gcgcgacgcg ccgtgggtgg
     9541 gaggcttcca tcccgtgtcc tatgggctca cgcacgcctg gatgatgaac ggcaagccga
     9601 acaacatggc gcgcaacaac ctgaagtacc tgcgcgtgga catcgagcag cgcgcggcgc
     9661 tgcgccgcga gtggaaccag cccgtcgtct ggcccctggg cctgctgctg ctcgccctcg
     9721 tcgtctcggc aatacccgcg gtgcgcagct accggcggcg cgagcgcatg gccgcgaagc
     9781 ccgcgtagat gctcgcctac atcatccgtc gcacgctgta cgcgatcccg atcctcgtcg
     9841 gcatcaacct gatcaccttc gcgctgttct tcctggtgaa cacacccgac gacatggcgc
     9901 gcatgcagct cggcgtgaag cgcgtcacgc ccgaggcgat ccagaagtgg aaggccgatc
     9961 gcggctacga caagcccctg ttgtggaacg ccggcgcgcc cggtgccggc aggttcaccg
    10021 agaccatctt cttcgagaaa tcggtgaagc tgttcgcttt cgacttcggc cgcgccgagg
    10081 acaaccgcga catcggcaac gagatccgta cgcgcatggg cccgagcctc gcgctcgcca
    10141 tccccgtgtt cctggtgggc ctcgcggtga acatcacctt cgcgctcggc atggcgttct
    10201 tccgcgccag ctacctcgac ctgtggggcg tggtcctgtg cgtggcgatg atgagcattt
    10261 catcgctgtt ctacatcatc ggcgggcagt acctgatctc gaagctgtgg cacctggtgc
    10321 cgatctcggg cttcgccgac ggcaccgatg cgctgaagtt cctcgtcctg ccggtggcga
    10381 tcggcgtgat cggcggcatc ggcgcgggca gccgctggta ccggacgctg tttctagagg
    10441 agatgggcaa ggactacgtg cgcacggcgc gcgccaaggg gctggccgag tcggtggtca
    10501 tgttccgcca cgtgctcaag aacgcgatga tcccgatcct caccggcgtc gtcgtcgtga
    10561 tcccgctgct gttcatgggc agcctgatcg tcgagtcgtt cttcggcatc ccggggctgg
    10621 gcagctacac catcgacgcc atccaggcgc aggacttcgc cgtggtgcgc gccatggtgt
    10681 tcatcggctc ggtgctctac atcgtcggcc tgctgctcac cgacatctcc tacacgctgg
    10741 tcgatccgcg cgtgcgcctc gcctagcatg ttggtcttcc tgccgaccga cgtcctgatc
    10801 tggctgctgg tggcggcggt ggccaccttc gcgtggtact cgagccggcg cgcgcacctt
    10861 gccgcgccct gggcgcgcgt gttccgcagc cgcatcgcgg tggcttgcgc ggtggtgctc
    10921 ggctgcttca tcgccgtggg cctgctcgat tcgctgcatt accgtcccaa gctgccgcag
    10981 aagggcccgc aggcgccggc cgcctattcg gtgcaggtca actcggcgct cgatgcgctg
    11041 ctcacgccgc tgcgcacgcg cctggagaag acctactccg caccgctcgc cacgcgcctg
    11101 taccagaagg agagcgtgga gcggcccggc ggcattaccg tgcgcgaatt cccccgcctg
    11161 gcgcacggcg gggcgcattt ggaggatgag tccgagtggg gccgggacgt ggccgtgcgc
    11221 gtcctggcag ggctcgcggg cgcggccctg ctgtgggggc tcgtggtgtt cgccggacgg
    11281 cgcaccctgg cacggcgctg gccggccgtg ccttggggcg cagccgccgc cgcgctcggc
    11341 gcggtactgg cgctggccgg cccggcggcg gtgctcgccg agggctatca cgtgctcggc
    11401 accaatcagg tgggcagcga tgtcttctac caggcgctga agagcatccg cacgagcctt
    11461 gtcatcggca cgctcaccac gctggtgctg ctgccgctcg gcatcgccgc cggcattgcc
    11521 gcgggctact tccgcggctg ggtggacgac gccatccagt acgtctacac cacgctcaac
    11581 tcgatccccg gcgtgctgct gatcgccgcc tcggtgctga tggtgcaggt gtacgtggac
    11641 acgaacccgg agctgttccc gaccgcggcg cagcgcgcgg acttccgcct gctcgcgctg
    11701 tgcgcgatcc tcggcgcggt gggctggacc gggctcgcgc ggctgctgcg cggcgaggcg
    11761 ctgaagctct ccgagctcga gtacatccag gcggcgcacg ccttcggcgt gtcgcacgcg
    11821 cgcatcctgg cgcgccacat cctgcccaac gtcgcgcaca tcgtcatcat cacgctggtg
    11881 ctcgacttct ccggcctggt gctcgccgag gcggtgctgt cctacgtcgg cgtcggcgtc
    11941 gacccgtcca ccatcagctt cggcaccatg atcaacgccg cgcgcatgga gatggcgcgc
    12001 gagccgatgg tgtggtggtc gctcgccgcc gcgttcgtct tcatgttcgc gctggtgctg
    12061 gcggccaatc tcttcgccga cgcggtgcgc gatggcttcg acccgcgggt gcgcgtatga
    12121 gcgccgtgct cgaggtcaag gggctgcgca ccgagctcga cgcgcccgac ggggtggtgc
    12181 gggccgtcga cggcgtcgac ctcgagatcc ggaagggcga gtgcttcgcg ctggtgggcg
    12241 aatcggggtg cggcaagtcg atgaccgcgc tgtccatcct gcggttgctg cccgacgtgg
    12301 ggcgcatcgc aggcggcagc gtgcgcctgg gcgaggtgga cctgctcgcg ctgcccgagg
    12361 ccgcgatgcg cgcggtgcgc ggccggcgcg cggcgatgat cttccaggag cccgccaccg
    12421 cgctcaatcc cgtgctcacc gtgggccggc aggtcgcgga agtgatcgag cgccacacga
    12481 gcctgcgcgg cgccgcagta cgcaagcgcg ccctggaatt gctccacgcc gtgggcattc
    12541 ccgaggccga gcgccgtgtg gacgagtacg cgttccagct ctccggagga ctgcgccagc
    12601 gcgtgatgat cgcgggcgcg ctcgcggtgg atcccgaagt gctgatcgcc gacgagccga
    12661 ccaccgcact cgacgtcacc atccaggcgc agatcctcga gctgctgtcg aagctgcagc
    12721 gcgagcgcgg catggcgctc ttgctcatca cgcacgacct cggcatcgtg gcgcagatgg
    12781 ccgaccgcgt ggcggtgatg tacgcgggcg agatcgtgga agtcgcgcca cgcgaagcgt
    12841 tctttcgcgc gccgcagcac ccgtactcgc agaagctctt tgcggcgctc cccagcgcgg
    12901 cgcagcgcgc ggggccgctc gcgcagattc ccgggcaggt gccgccgctc accggggaat
    12961 tcaccggctg ccgcttcacc gagcgctgcg aattcgcttt cgagcgctgc cggcgcgagg
    13021 cgccgaagct gctgcaggtc gatcccgcgc accgcgcgcg ctgccacctg cgcgagaagg
    13081 accgagcggc gccgcgcgcg gcggccacgg gcgcgcccga ggcgcgcgcc gggggcgcgg
    13141 cgcgcccgct gctgcaagtg caggacctga aagtgtactt cccgatccgc cgcggcgtcc
    13201 tgcggcgcac cgtgggctac gtgaaggcgg tggacggggt cacgctggcg gtgcccgagg
    13261 gccgcacgct cgcgctggtg ggcgagtcgg gctcgggcaa gaccaccgtg ggaaagagca
    13321 tcctgcagct catccggccc accgccggca gcgtgcgcta cgctggcgag gagctcacgg
    13381 ggcttggccg cgccgcgctg aagccgcacc gcgcgcgcat gcagatcgtg ttccaggacc
    13441 cctacgcttc gctcaacccg cgcatgcgcg ttgccgacat cctgctcgaa ggcatgcgcg
    13501 ccctgggcgt gggcgccggc gaggccgacc gggtgaagcg ctgcgacgcg atcctcgagc
    13561 aggtcgggct gcccgccgcg gcgaaaagcc gctacccgca cgagttctcc ggcgggcagc
    13621 gccagcggat cgccatcgcg cgtgcgctcg ccgtcgagcc gcggctcatc gtctgcgacg
    13681 agcccaccag cgcgctcgac gtctcggtgc aggcgcagat cctcaacctg ctgcgcgagc
    13741 tgcaggagcg cctgggcctc gcctacctct tcatcacgca caacctggcg gtggtcgagt
    13801 acctcgcgca cgaggtggcg gtgatgaccg cgggccgcat cgtcgagcag ggcgcggccg
    13861 agcaggtgct gcgcgcgccg cgccacgcct acacgcgggc gctgctcgag tcggtgccga
    13921 agatcgacta gaagatggtg aggcgctggc cggcgcggat gtggcttccg atccggttcc
    13981 agcgctgcag gtccttcagc gggatgccgt agcgccgcgc gatgctccag ggcgtctcgc
    14041 cacggcgcac gacgtgggtg cggccgcccg ccgggacggg cggggcgtac atgatgggca
    14101 gtcgcgcgaa ggattcgccg ccctcgatcg gcaccacgag gagctcgggc atgcgccgcg
    14161 agcgcgggtg gatgccgttc acctcgcgca gctgcgcaac cgacaggccg ggccggcgcg
    14221 cgatcgactc cagcgagtcg ccgcgcttcg gcttgtaggt cttccaggac acgagctggt
    14281 cgtcctcgag cttctgcagg ttgtcgtgga acacgtccac cttgtccgcc ggcagcacga
    14341 tgcgcggtgc caccgccgag cggatcacgg ggcggctgaa gcccgggttg aggttgacga
    14401 aatcctccac cgacatctcg gcgagctcgg cggcgagctt gacgtcgatg tcgcgcgtct
    14461 tggtgacggt ggcgaagtag ggctcgttgg ggatcggcgc gagggcgatg ccgtgcgccg
    14521 ccgggttggc gatcaggttc ttcagcgcct gcagcttcgg cacgtagccg cgcgtctcgc
    14581 gcggcatcgg cagcgacagg tagtcggtgg gcttgccggc cttgcggttc ttggcgatgg
    14641 cgcgcgcgac attgttctcg ccccagttgt aggaggcgag cgcgaggtgc cagtcgccgt
    14701 gcatctggta caggtcgcgc aggtaatcga gcgcggcgtc ggtggacgcg accacgtcgc
    14761 gccggccgtc gtaccacatg ttttgcttca gctcgtagcg ccggccggtg ccgggaatga
    14821 actgccacag gcccgaggcg tgcgagcgcg agtaggccat cgggttgaac gcgctctcga
    14881 acatcggcac cagcgcgagc tcggtgggca tgttgcgctt ctcgagcgcc tcgacgatgt
    14941 ggaacagata gcgccggctg cgctcgaaca cgcgctgcag gtagtcctga ttggcgatgt
    15001 accagcgcgt gcgctcgtcg accagcggcg agcggatgtc gggcatggcg aagccgcggc
    15061 gcagccgctg ccacaggtcg tcgacgcgct gcgtgcgatc gagcttggcg atcgcctgcg
    15121 gctcggcgag ctcgcgcttc tcgccctcga acgggggcag ccgctcggcg tcgagcaggc
    15181 gcaccacggg cagcgggggc ggcggcaacg gcggcaacgg caacggcttc ttcgccacgg
    15241 gcagcggcgc cggatcggcc ggcggcacgg tctcggggat ggcgaggctg accggggggg
    15301 cttcgggcgc cggctcgggc accagggcgc acgcctgcag cagcagcgcc gcagctacga
    15361 aaatacttcc ggaaaatccc ctaagtccct gcatgcaggc cagatactac ctgctcagcc
    15421 acggttcttc cagtcccgga tggcggcgaa cacccgcacc gggtccgaga cgcgcgaacc
    15481 gaggtacttg ttggcggatt cgaccaccgc gggctccgcc cagcgcagga acgggttggt
    15541 ggcgcgctct tcgcccaggg tcgagggcag cgtcggcttg cccgcgtcgc gcagcttgcg
    15601 gtcgcgcgcg aggcgcgtgg cgagcgcggc gttctgcggc tcgacctgct gcgcgaacgc
    15661 gatgttggcg agcgtgtatt cgtgcccgca gtagaccttg gttgcgtcgg gcagcgcggc
    15721 gagcttggcg agcgaggcca tcatctgctc gggcgtgcct tcgaacagcc gcccgcagcc
    15781 gcacgcgaac agcgtgtcgc cgcagaacag gctccctgcg ccataatagg cgatatgcgc
    15841 tcgcgtatgc ccaggaatat cgagcaccga gagcgtgacg ccgagctcgg ggatttgcac
    15901 cgtgtcgcct tcgcgcacgg gccgcgtcag cgtggcaatc ggctcgccct tgggaccgta
    15961 gaccggcacc ttcctcaggg ccagcagctc cggaatgccg ccgacgtgat cggggtggtg
    16021 atgcgtggcg aggatggcgc agagctcgag cctctcgcgc tcgaggtact cgagcaccgg
    16081 gtgcgcctcg cccgggtcga ccaccgcggc gtggcgcgcg tcgcgcagcg tccagatgta
    16141 gttgtcgcgc aaggccggta gcggaacgat ttcgagcata tggatcgcca gtccttcgtg
    16201 tcggaagccg cggccaagta taacggcgag cgcgcgtcct tttccggctg gtgcgcgacg
    16261 ccgctcgggc gctacgtgct cgactgggag atgcagcagt tcgacaacgc ggtcgaggac
    16321 gtgttcggct accgcgcggt gcaggtcggt ctgccggagt tcgcgttcct gcgccagaac
    16381 cgcatcccgt accgcttcac cgtggcgctg gaagagggcg cggcgcttgc cgccgacccg
    16441 ctgcagctgc cgcttgcctc gcagagcctc gacctcgtcg tgctgccgca cgcgctggag
    16501 tcgcacctcg acgcgcacag cgtgctgcgc gaggccgagc gcgtgctgcg gcccgacggg
    16561 caggtggtga tctccggatt caacaacgcg tcgctgtggg gcctgcggcg atggtttgcc
    16621 ggccgcaacg ccggctcgcc ctgggacgca cgcttcatcg ggctgctcag gctgaaggac
    16681 tggctgcagc tgctgtcgtt cgacctgaac ggcgggcgct tcggctgcta cgtgccgccg
    16741 cttacgcagc ccgcctggcg cgagcgcttc gcgttcatgg aaaaggtggg cgcgcgctgg
    16801 tggccgatcc tcggcggcgt gtacgtggtg cgcgcggtga agcgcgtgca cggcatgcgc
    16861 ctcatcaccc ccgcctggcg caaggaacgc aagcgtcggc gggcgctcgc ggcgatgccg
    16921 caaaagaacg ggcacgtgac gcagcggcgg catgatcgct gaggcggtgg aggccttcgc
    16981 cgacggcgcc tgccgcggca acccgggccc ggggggctgg ggcgtgctgc tgcgcgtggg
    17041 cggccgcgag cgcgagctgc acggcgggga agcgcaaacc accaacaacc gcatggagct
    17101 caccgcggtg atccgcgcgc tggaggcgct cgcgcccggg agctcggtgc gcgtgtacac
    17161 cgactcgcag tacgtgcaga agggcatcac cgcgtggatc cacgactgga agcgccgcgg
    17221 ctggcgcacc gcggacaaaa agccggtcaa gaaccaggac ctgtggatgc gcctcgacga
    17281 gctggcgcgc gcacacgacg tggagtggca ctgggtgcgc ggccacgccg ggcacccgga
    17341 gaacgagcgc gccgacgcgc tcgccaacag gggcatcgac agtggcgtct aggaaggagg
    17401 cggatgtccg ttcctaggca ggtcgtgctc gacaccgaga ccacgggcct gtcggcgagc
    17461 ctcggcgacc gggtgatcga gatcggctgc atcgagctgc tcagccggcg cgtgagcgac
    17521 aaccacttcc accgctacgt caatccggaa agaaagagcg acgagggcgc gctgcgcgtg
    17581 cacggcctca ccgaggagtt cctcgccgac aagccgaagt tcgccgagat cgcgggcgcg
    17641 ttcctcgact acgtgcgcgg cgccgagctg atcatccaca acgccgattt cgacgtcgag
    17701 ttcctcgacc tcgagctgga gcgcgcgggc ctcgggcgcc tgaaggagca cgtcgccggc
    17761 atcgtcgaca cgctggcgct cgcgcgcgag ctgcatcccg gtcggcgcaa ctcgctcgac
    17821 gcgctgtgcg agcgctatgc ggtgaacaac gcgcaccgca cgctgcacgg ggcaatcctg
    17881 gatgcgcggc tgctggccga ggtctacctc gcgatgacgc gcgggcagga cagcctggcg
    17941 atggacctgg agaccgccgc cgagcaggcc gcggtcaccg cgcgcatcga cgcctcgaag
    18001 ctcgtcgtgc tgcgcgcctc ggaagaagag gagcgcgcgc acgaggcgta cctcgacggc
    18061 atggcgaagg aggcgggcgg gaagatcgtg tggcgcggcg cgtagctacg ccgtctggcc
    18121 gccgcaggag ctgccggcgc ccgcggtgca gccgaagcag tggcgcgcgg tgacgatcgc
    18181 gcgccggcgc caggcccgct cgtcgaagtc gcggatgtgg gcgccggccg ctgcatcgag
    18241 cgccagctcg agcatctggt tgaagtcgca gtcgtagagc cgcccgtccc agccgaccga
    18301 gagcgtgctg cggcacatga cgccggcgag cgcgccggga ttgaaggcgt tcgccagccg
    18361 ctccaggtag gcctcgaggt ttcccgtgtc ctgcagccat tcgagaaagc gcgcgatcgg
    18421 catgttgttc agcacgaaca ggcggtcgaa cgtgaggccg aaattcctct gcagcccctc
    18481 gcgccactcg cgctccacgc tagcctgggc ggcgccgagg aaggcgccgg cggggttcga
    18541 gaccagcgtc aggcgccgct tcgggtcgcc cacgccgtag ccggcggcgt tcaggatgcg
    18601 gagcgcgcgc agcgagcgct cgaacacgcc gtcgccgcgc tgcgcgtcgg tattcgggcg
    18661 gcggtagtgc ggcagcgagg cgacgatctc gacgttgcgc tcggcgagcc agtccacgag
    18721 gccggcattg cgcggcagca gcagcacggt aaggttgcag cggtccatga cgtgcttgcc
    18781 ggcgtcgaac gcggcctgca cgacgtcgcg aaagcgcggg tgcagctcgg gcgcgccgcc
    18841 ggtgaggtcc accgtgtgcg ccccggtggc gcgtaccgcg gcgattaccg cgtccaccgt
    18901 ggcgtcgtcc atcacggcgt cggtacggtc ggggccggca tcgacatggc agtgccggca
    18961 agtcatgttg cagagcttgc cgaggttgac ctggaagatc tcgagctgcg cgggcgcgag
    19021 gtcgggcgcc attgcgccga actcgcggcg cagcggcacg ccggcgagga gcttgagctg
    19081 ctcggcgggc gccgcgagcg gggcctggcg ccgcttgagg ctctgcgcgg cgcgcggcga
    19141 ggcgcggtcg tcggcgagct cgaccgcgat ctcctccaga cgcaagggaa tgctggccgg
    19201 gcgcaccgct acatgcccct cttcttcgcc tgctcgcgca tctgcatgcc gtgcaccagc
    19261 gacgagccgc cgcggatggc gaccgcgacg tgcagcgcct cggtcatctg ctcgaggtcg
    19321 gcgccctttt gctggcaatc ctgggtatag gcgtcgatgc agtagggaca ttgcacggtg
    19381 tgcgccacgg cgagcgcgat gaggctcttt tcccgcgcgc tgagggcacc gtcggcgaat
    19441 accgcgccgt agtagtcgaa gaacttcttg ccgagctcgg ggctggcctc ggaaaccttg
    19501 ccgaagtcgg cgaggtgctg gggatcgaaa tagtggttca tgacggcctt gtcgtcgttg
    19561 gtcgaagagg caggcgccga gcttacagta ggctaacgcg cgaagatcac gaacagcgct
    19621 acgccaagcg tcgccgccga caaggcgatg gcggcgaggc acagcaggta cgcgcggcgc
    19681 agccgcgcct ccgcgatcgc cgccgcctgg tgcaaggccg ataccgtgcc ctggagctgc
    19741 tcggcgagcg ccttgatgtt gtccgcgttc tgcgaggcgg ccgcctgcag ctcggcgatc
    19801 tgctgttgca tgagctgcgc ctggttggcg gcctcggcgc tcttcttggt gaagacgggc
    19861 gcggcgacgg tgatcagggt gccgacgtgc ggcaggacgg ctttcagcgc gggcaggaac
    19921 caggcggcca tcggcgtagt gtcgcagatt cagccggccg cgcccccgcg cgccgcgcgc
    19981 agctcgtcga tgacctgcgg attgaccagc gcgctgaggt cgcccggatt ctcgccgaga
    20041 atcgcggcgc ggatcacgcg gcgcatgatc ttgaggttgc gcgtcttggg cagctcggac
    20101 acgaagagca ccgccttcgg cctgaacggg gtgccgagcc cgcgtgtgac gacggcctcg
    20161 agctcgcttt gcaatgacgc ggccggtgcg acacccggct tgagtgcgca cgcgagcacc
    20221 agcgcctgcc ccttgatggg atcgggcacg ccgatcgccg cggcctcggc gagcttgccc
    20281 gtcgccatga gcagcgcctc gatctccgcg gggccggtgc gcttgcccgc gatcttcagc
    20341 gtgtcgtcgg agcggccgcg gatgtaccag tagccgtcgg cgtcgcgcac ggcgaagtcg
    20401 ccgtggtgcc acacgcccgg ccaggtgctc cagtagcttt cgatgtagcg cgcgtcgtcc
    20461 tgccacaggc cgcgcgtgag gccgatcggc ggccggcgca tgacgagctc gcccaccgtg
    20521 ccgggcggta ccggcgcacc gcgctcgtcg acgacgtcgg cgccggcgcc cgggatcggc
    20581 gtgttgaacg agcagggctt gagcggccgg agcggcgtgc agccgagaat accgatcatc
    20641 tcggtgccgc cggaaaagtt gagcagcggc acgcggccgc cgccgacgcg ctcgaacagc
    20701 cagtgccagg cgtccggcgt ccaggcctcg ccgctcgaga tcatgacgcg caggcgcgaa
    20761 aggtcgtgcc ggcgccaggg ctcgcagccc tcggccatga aggtgcggat ggtagtcggg
    20821 gcgaccccga ggtaggtcac cccgtgctcc gcggcggcgc gccacatgcg gtcgggctcg
    20881 ggaaagttgg gggcgccctc cacggcgacg agcgtgccgc ccatgacggg cgtgccgtag
    20941 gcgagcatcg gcccgaccac ccagcccatg tcgctcatcc acaggatgcg gtcgctcgac
    21001 ttccagtcca tggtcagcca catgtcgagc agcactttca tcgggaagcc gacgtgcgtg
    21061 tgcacgacgc ccttgggctt gccggtggtg cccgatgtga agagcaggag atagggcgcg
    21121 tccgcttcca ttggctcggt cggcgcaccg gcgccgcggc cggcggcgag ctcgtgccac
    21181 cagtggtcgc gccgcgcatc ccaggcgacg gcgttgcgcg cgtggcgcag cacaatcacg
    21241 tgctcgagcg cgggcgcgcc ccgcgcggcc tcgtccacga cggccttggc gtcgacgagc
    21301 ttgccgcgcc gcaggctgcc gtcgacggtc accagcgccc gtgcgccgcc gagcgcgagg
    21361 cgatcggcca tggcgtcggc gccgaagccg gagaacagcg gcatgacgat ggcgccgatc
    21421 ttcgggatgg cgagcgcggc gaccaccgtt tcgacgagac tcggcaggta catcgcgatc
    21481 acgtcgccgc gcccgaggcc gagcgcgcgc aggccggtgg cgagcgcgca tacctccccg
    21541 tcgagctctc gataggtgag gctgcgccgc gcgccgtctt caccctccca aacgagcgcg
    21601 gtcttgccgt aggtgggcgt gccgcgccag cggtcgagcg cgttcagcac cacgttggtc
    21661 gtgcccccca cgcaccagcg cggccaggcg atgcccgcgg attcgtccag cgcgcgcgtg
    21721 tagggcgtgt aaaaggcgaa gccggccgcg ccgatcagtg cgcggtagaa gcgctcgggc
    21781 tcacgctcgg cgcaggcgag cagttcctcg tagcccgcga cgccgagcgc gcgcatgaac
    21841 gcggtcgcgt tcgcctgcgc caccccctgc gggtcgggcc gccaggcgat gccgtcgctc
    21901 agcgtcacgc ctgcgccggg aacgcccgcg ccagcaggtt gtacagccag ccgaagatcg
    21961 cgccggcgat ggcgccgtcc accgcggcgt agagcgtgcc cacgatgacc gagccggcgc
    22021 cggtgccggg acggtagccg ggatagatcg aggccgagag ctcgaggaag gccgagccgt
    22081 accctggcga ggcgaggttg gcgagcgcga cgatgagcag ggcggcgccc cagaacaatc
    22141 cggcggtcag tgcgagcgcg gtgatgttga gcttcataga acctcctcta ctcgaacgat
    22201 gaagggcaac gcgggccgga ttgtcgcgac ggtcatcgcg gaacagccgt accccaataa
    22261 ttgaagcttg ccgggcgcca gcccggcaag tacatggtgt ccaggctcgt gatgcgaaac
    22321 cctgcggtct cgatgagcgc cggaatcggg cgattcaggt tgcagccgcc cccgagccgc
    22381 ttccacagcg gattgagccg atcctgccag cgacgcacgc tcgcatccgg cgccgccccg
    22441 tgctcgcaga agacgagctc cccgcccggc cgcaggaccc ggctgatctg ccgcagcgcc
    22501 gccgtcgtgt caggaatggt gcacagcgtg taggtcacca caacggtgtc gacgctgttt
    22561 tcatcaagcg ggatcgcgtc gctcggcgct tcgatgaact ccacctcgaa tgggcgcgcg
    22621 cccgcggccc gcttggccat ccgcgtcatc tcgggcgccg gatcgaggcc ccagaccttg
    22681 ctcacctttg cggcgtcata gaaggagagg ttcaagccgg tgccgatgcc gacctcgagt
    22741 acgcgcccgc gggcgagcgg caccaccttc tgccgctggc gcatcgccgg cttcaggccg
    22801 caggcgaagt tgacggcccg cggaaggatg aagcggctat acagtcccaa ggacttctcc
    22861 tggcccgaca cgaaggtcga caagcagcgc gaaagcgtcg cgcacggcgc attcgacggc
    22921 gcaggggtct gcggaggtgc tgccgccgga agtgccgctg gtcatggcca atatccctcg
    22981 ggtggacgcg gcaaggatat acccctgggt aaccacggcg atatactttc cttcctcatg
    23041 cagaagtcca gtgcctacga gcaggacctg taccgcaacc ccgccaatta cgccccgctg
    23101 acgccgctcg ctttcatcga gcgcgcagcc agcgtgtacc cgcggcgcac cgccgtgatc
    23161 cacggcgcgc gccgctttac ctggggcgaa acctatgcgc gctgccggcg gctcgcctcg
    23221 gcgctggcca agcgcggcat cggcaagggg gacaccgtgg cggcgatgct cgccaatacg
    23281 cccgagatgt acgagtgcca cttcggcgtg ccgatgacgg gcggggtgct gaatacgctc
    23341 aacacgcgcc tggacaccga cgcgatcgcc ttcatgctga accacggcga agcgaaggtc
    23401 ctgatcaccg acaaggagtt cgcgccgacg atcgagggtg cgctgaaaaa gctcgggcgc
    23461 gcgatcgagg tgatcgacgt cgaagacccc gagttcgagg gcggcaagcg cctcggcaag
    23521 gcgacctacg agcagctgct cgaggaaggc gacccggcgt tcgactggcg ctggcccgag
    23581 gacgagtggc aggcgatctc gctcagctac acctccggca ccaccggcga tccgaagggc
    23641 gtggtgtatc accatcgcgg tgcgtacttg aatgcggtgt gcaacatcct cacctggggc
    23701 atgccgcacc acgcggtgta cctgtggacg ctgccgatgt tccactgcaa cggctggacc
    23761 tttccctgga ccatggcggc gaacgcgggc accaacgtgt gcctgcgcag ggtcgaggcg
    23821 gcgaagatct tcgagctgat caaggcgcac aaggtgtcgc actactgcgg cgcgccgatc
    23881 gtgcattcga cgctggtcaa cgcgccggag gcgatgaaga aggggatcac gcacaaggtg
    23941 tcggcgatgg tggccgcggc cgcgccgccg gcggcgatga tcgaaggcat ggagaagatg
    24001 gggttcgacc tcacgcacgt ctacggcctc accgaggtgt acgggcccgc caccgtgtgc
    24061 gcgaagcacg aggagtgggg cgcggccgac atctccgagc gcgcgcgcct gaacggccgc
    24121 cagggcgtgc gctacctgat gcaagagggc ttgagcgtca tggacccgca gaccatgaag
    24181 ccggtacccg ccgacggcga ggccatgggc gagatcatgt tccgcggcaa catcaccatg
    24241 aaggggtacc tgaagaaccc gaaggcgacg caggccgcgt tcgccggcgg ctggttccac
    24301 tcgggcgacc tcgccgtgat gcagcccgac ggctacgtaa agatcaagga ccggtcgaaa
    24361 gacatcatca tctcgggcgg cgagaacatc tcctccatcg aggtggagga cgtgctgtac
    24421 cgccatccgt cggtgctcgc cgcggcggtg gtggcacgcc ccgacgagaa gtggggcgag
    24481 acgccgtgcg cgttcgtgga gctcaagccc gaggccaagg cgagcgaaac cgagctgatc
    24541 gagttctgcc gccagcacct cgccaggttc aaggcgccgc gcgcggtggt gttcggcgag
    24601 ctgccgaaga cctcgaccgg caagatccag aagttcgtcc tgcgcgagaa ggccaagtcg
    24661 gcttccgcca tcgagtaagg agaatcgcat gagcgccgta ccccaggccg agcagcccgt
    24721 cctgctgcgc gaagacctca acggcgtcac gacgctgacg ctcaaccgcc cgcagcagat
    24781 gaacctgctg acccgcgaga tgatcgaggc gctgcaggcg gccttcgact cgatcgcgaa
    24841 ggatcccgac gtgcgcgtgg tggtgattgc cgccgcgggc aggggctttt gcgccggcca
    24901 cgacctcaag gagatccgtg cgctggagga gctgccgaag atcgaggggt tgtttttcgc
    24961 ctgcagccgc atgatgctga ccatcccgat gctgccgcag ccggtgatcg cgcgcgtgca
    25021 gggcgcggcg gccgcggcgg gctgccagct ggtcgcgcag tgcgacctcg cggtggcctc
    25081 ggaggccgcg aaattcacca cccccggcgt cacctggggc ttttactgct cgacgcccgg
    25141 cgtggccgtc ggccgcaacc tgcagcgcaa gcacgcgctc gagatgctgc tgaccggcga
    25201 cctggtggac gcgaagcgtg cgctcgagtg gggactcgtg aaccgggtcg ttccggcgca
    25261 tcagctcgag gcggagaccg gaaagctcgc gcgccagctc gccgacaagc cgccggcgac
    25321 ggtggcggcg ggcaagcgcg ccttctacca gcagatggag atgggcacgg aaaaggccta
    25381 cgagctggcg agtcacgtga tctcctccag cttcgcgcat cccgagggcc gcgagggcat
    25441 ggacgcgttc atcgagaagc gctcgccgcc cggccagaag acctaggatg ggccgctact
    25501 acgacgactt cgcggtcggc gacacctaca agcactggcc gggccgcacc atcagcgcgg
    25561 ccgacaacac ctggttcacg ctgctcacca tgaaccagca cccgatccac ttcgacgagg
    25621 agtacgcgaa gagctcggag ttcggcaggc ccctggtgaa cagcgcgctg accctcgcca
    25681 tcgtggtggg catgagcgtc cgggacgtga gccagaacgc catcgccaac ctcggctgga
    25741 ccgacatcgt gatgccggcg ccggtgttca acggcgacac gctctacgcc gagtcttcgg
    25801 tgctggaaaa gcgcgagtcg aagtcgcgtc ccggccaggg catcgtcacc gtgacgacgc
    25861 gcggctggaa gcaggacggc accacggtca tgactttcaa gcgttccatc ctcgtcccga
    25921 agaaaccatg agcgaagaca cgcagatcct ggacgccatc ggcaagtggc tggagcgcga
    25981 cgtgcgcccc aaggcgcgcg agctggagaa gaaggacgag tatcccgaga agatggtgga
    26041 gcagatgaaa gagctcggcc tgttcggcgc cacgatcccg gagcagtacg gcgggctcgg
    26101 gctctcggcc tcgacctacg ccaagatcgt cgcgcagatc tcggaagtct ggatgtcgct
    26161 caccggaatc ttcaactcgc acctcatcat ggccgccgcg gtggcgcgct acggcaccga
    26221 cgcgcagaag cagaccttcc tgccgaagtt cgcttcgggc gaggtgcgcg gcgggctcgc
    26281 gctcaccgag cccgactgcg gcaccgacct gcaggcgatc cgcacgcgcg ccgtaaagca
    26341 gggcgatcac tacgtcatca acggcaccaa gacctggatc tcgaacggca tcaagggctc
    26401 gtgcttcgcg ctgctcgtca agaccgaccc gaacgcaagc ccgcggcaca agggcatgac
    26461 gatgttcctc gccgagaagg gcaagggctt tcgcgtgtcc aagaagctgg agaagctcgg
    26521 ctacaagggc atcgactccg ccgagctcat cttcgaggac taccgcgtgc cggccgcgaa
    26581 cctgatcggc ggcgcggagg gcaagggctt tcaccatgcc atcggcggcc tcgagctcgg
    26641 gcgcatcaac gtcgccgcgc gcggcgtggg cgtggcgaac gccgcgctca aggacgcggt
    26701 ggcgtactcg caggtgcgca agaccttcgg caagccgatc tgcgagcacc aggcgatcca
    26761 gctcaagctc ggcgagatgg cgacgcgctg cgaggcggcg cggctcctgg tcgagaacgc
    26821 ggcgcgcgcc tacgacgcgg gcgagcgctg cgacatggaa gccggcatgg ccaagtactt
    26881 cgcctcggag gcggcgctcg agaactcgat cgaggcggtg cgcatccacg gcggctacgg
    26941 ctactccacc gagttcaacg tcgagcgcta ttaccgcgac gcgccgctca tgtgcatcgg
    27001 cgaaggcacc aacgagatgc agcgcatcat catcgccaag cagctcatcg cgcggcaccc
    27061 ggcctgatgc tgcccctcgc gggcctgcgc gtcatcgccg tggaggtgta cggcgccggg
    27121 cccttcggca gcacgcacct cgccgacctc ggcgcggagg tgatcaagat cgagccgcgg
    27181 gaaggcggcg acacgtcgcg cgcggtgggg ccgttcatgc tcggggagga cgacagcgtc
    27241 ttcttccagt cgctcaaccg caacaagaag tccctcacgc tggatctcaa gcacccgaaa
    27301 ggcaaggaga tcctggcgaa gctcgctgcc accgcccacg gcctgatgag caacttgcgc
    27361 ggcgaccagc cggaaaagct cggcctcacg cacgcggcgc tcgccagggc gaacccgaag
    27421 ctcgtctgcg cgcacctctc ggcctacggg cgcgacggtg cgaggaagtc ctggccgggc
    27481 tacgactacc tgatgcaggg cgaggccggc ttcctcggcc tttcgggcga gcccgacggg
    27541 ccgcccgcgc gcatgggcct ttccatcgtc gactactcga ccggcacgat gtgcgcgctc
    27601 gcgctcctcg ccgggatcct ggaagcgcgc aagacgggca ggggccgcga cctcgacgtg
    27661 tcgctctacg acgtggccat gcacctgctc aactacccgg cggcgtggta cctgaacgag
    27721 ggcctcgtca ccgagcgcgt ggcgcgctcg gcgcatccgt tcatcgtgcc atcccagctc
    27781 taccggacga aggacggctg gatcttcgtc atggcgcaga accagcgctt ctgggagcgg
    27841 ctcgccgacg cgctcgggcg gcgcgacctg aaggcgaagt acccggactt cgccgcgcgg
    27901 cgcgagcacc gcgacgagct gacccaggtg ctcgacgccg agttcgcgaa gcaaacgacg
    27961 gaaagttggc tcgcgaagct gcgcggcacg atcccctgcg gcccggtgta cgacctgccg
    28021 caggcgctca acaacccgta cttcgtcgag cgcggcgggg tgcaggtgtt cgaccatccc
    28081 gacaagccgg gcttcaagct ggtggcgagc ccgttcagga tcggcgagcc gctgccggcg
    28141 cggcccgccc ccaagctcgg cgagcacacc gaagcgctgc tgaaggagct cggctacgcg
    28201 gacgccgaga tcgcgcgact gaaggccgcg agggcggtgt gatgaagctc gagggcatcc
    28261 gcgtcatcga cctgtcgctg ttcctcccgg ggccgctcgc cacgcaattc atggccgatc
    28321 acggcgccga ggtgatcaag gtggagtcga tccacgaggg cgagcccaat cgcgagatcg
    28381 gcgccaagcg cggcggcacg agcgtgtact tcgccaacac gcaccgcggc aagcaaagcc
    28441 tcgcgctcaa cctcaaggac cccgcgggcg tggaagtgct cctgcgcctg gccgagaagt
    28501 ccgacgtcct tgtggaggcg ttccgtccgg gtgtcgtcga tcgcctcggc atcggctacg
    28561 cccaggtggc caagcgcgcg ccgcagctcg tctacgcgtc gatcgccgcg ttcggccagc
    28621 gcgggccgta tcgcgacatc gccgcgcacg acctcgccac cgaggcgatg accggcgtgc
    28681 tgtcgctcaa ccgcggcggc gacggcaagc cggcgatccc ggcgctcgcc aacgccgaca
    28741 tgctcgcctc gatgatgacg ctctcgggga tcctgatggc cttgcttcgc cgcaaggaca
    28801 ccggccgggg cgattacctc gacatggcga tggccgacgc gctgctcgcc tgcctgccga
    28861 acaacatggg cgtggcgatg accgagcggc gccagcccga cctgaagggc gagcgcgcgc
    28921 tcggcggcaa cgcgctctac aacatctacg aaacgaaaga cggcgactac gtggtgctcg
    28981 ccgggcagga gccgaagttc gcgaagaacc tgctcgagcc cttggggcgc gccgacctgg
    29041 tcgagctttg caagctgccg ccgggccccg ggcaggaccc ggtgcgcgcc ttcctgcgcg
    29101 agaccttccg cacgaagacg aaagccgagt gggtcgagtg gttcaagggg cgcgacgttg
    29161 cctttgcgcc gctgcgcacg ctgcccgagg tgctcgagga cgagcacttc aaggcgcgcg
    29221 gcatggtgct gagggatgat cgcggctggg atcatctcgg catcccgatc gcctttcgcg
    29281 aggagcccgg gcgcgtgcgc ttcgacgcgc ctgcgcacgg cacgcattcg agggcaatcc
    29341 tgcgtgggct cggctatggc gcggccgaga tcgcgcaact cgagtcgcgc ggcgtcatca
    29401 agagcgcttg agccggcatc tcgtcatcct caacccggtg gccgggcgcg gccgcgcgcg
    29461 ccgcgagtgg ccgcgcatgg cggacgcgtt gcgcgcggcc ggcgtggcgt tcgaggtggc
    29521 gcagacgagc actccgggcg aagccgagcg tctcgccgcg caaggcgcga gtcggttcga
    29581 cgcgctcatc gccgcgggcg gcgacggcac ggtccacgga gtcgtgaacg ggctgctcgg
    29641 cgcgggcggc acggccgcgc tcggcgtgct gccgctcggc agcggcgacg acttcgccaa
    29701 gatgctgccc gccggcgatc ccatcgcgcg catcgcgcgc ggggcggcgc gcgccttcga
    29761 tgccgggcgc atcgccgcgg gtgccacgcg gttcttcgcc aatggcatgg acgtcgggtt
    29821 cggcgcgcac gcctcgcgca acgtgcgccg cgtgccgcga cccttcacgg gactgggcgc
    29881 ctacctcggc gcgctgttcc tcaccctggt gcgctacccg aggctcgagc tgcggctgca
    29941 actcgacgaa ggcgaagcct tcgcgcagac caccgccatg accgcggtga tgaacgggcg
    30001 cgccttcggc ggcagcttcc acgtctgccc cgacgcgcgc gcggacgacg gcgagctgga
    30061 tctgctgatc gccgaaggcg tagggcggct ggcgatcctt ggcctcgtgc cgaggatcat
    30121 gcgcggaacg cacgggggcg acccgcggct gacgctgacg cgggcgagaa aggttctcat
    30181 cgagtccgag gcgccgctcc tggtcgaagc cgacggcgaa atcgtgttcg aggacgcgcg
    30241 ccggctggag atcgcgatct tgccgggggc gctgcgcgtg ctcgcgtagg gagagggccc
    30301 gcgccccgcg gagaggcccc gtattgataa ggatcaaggt gaccgggggc gcctcggcca
    30361 tcatgcccga tttccgcgcg tcccgacaac cgtgctccga tcgctcgtca tctggttcat
    30421 cgtccttgcc gccatcgcgg cctgggtgcc gctggtcgcg gtcgtgcgcg tgttcgaccg
    30481 ccaccccgcg cggctgcgca cgggtcgctg gtttcgccgg ctcggcgtcg tggtcacccg
    30541 gctcaacccg ctgtggaagc tgcgggtcga cggcatcccg gccggcgggc tgagggcgcc
    30601 ctgtgtcgtc gtcagcaacc accagtcgct cgccgacatc cccctgcttt cgcagctgcc
    30661 atgggagatg aagtgggtgg ccaagcgctc gctgttccgc ctgccggcga tcggctggat
    30721 gatgcgcatg gcgggcgaca tcccgctcca gcgcaccgac ccgcgcagcg gcgcgcgctc
    30781 gctgctgcat gcggcgcgct acttgcgcca gggctgtccg gtgatgttct tccccgaagg
    30841 cacgcgctcc cgggacggcg gcgtcgggcg gtttcacgac ggcgcgttcc ggctggcgct
    30901 gcgcgagcgg gtgaacgtgc tgccgatcgc gatcgacgga tcgcacgcct gcctgcccaa
    30961 gcacagctgg cagttcggcg ccgcccgcac catccgcgtg cgggtgctgc cggcgatcga
    31021 gatcgcaggt ttcggccccg accagaccga agcgctgcgc gatgcggcgc gccgcgcgat
    31081 catccgccag atcgcgctgt ggcgcgacgc cgacgagtcc gcggtcgacg ccgcgggaaa
    31141 ggcggccgcg gcaccgttcg ccgcgccgtc gcgggtttga gcatgcaccc gccgccgccg
    31201 ctgccgcacc tcgcgatccg gggcaagcgg ctgctgccga tcgtccaggg cggcatgggc
    31261 gtgggggtgt ccgcgcaccg tcttgccggc agcgtggcga gcctcgacgc cgtcggcacg
    31321 atcgcgagcg tcgacttgcg ccgcctgcac cccgatctca tggaacgcac cggcaggtcg
    31381 cgcgacaaga gcgcgatcaa cgccgccaac ctcgaagccc tcgaccggga agtgcgctcg
    31441 gcgctggggc tggcacaggg ccggggcctg gtcgcggtga acgtcatgcg cgcggtctca
    31501 cagtacgcgg actacgtgcg ccaggcctgc gaaagcggca tccaggccgt ggtcatgggc
    31561 gcggggctgc cgctcgacct gccggagctg accgcgggct tcggcgacgt cgcgctgatt
    31621 ccgatcctgt cggacgcgcg gggcacgacg ctgctgctgc gcaagtggat gcgcaagcag
    31681 cgcctgcccg acgcgatcgt gctggagcat ccgcgctatg ccggcggcca cctcggcgcg
    31741 gcggacgagg ccgacgtgct cagcccgagg ttcgatttcg ccacggtgat accggcggtg
    31801 ctggcgagcc tcaaggagct cgggctggag cgcgaaaaca ttccgctcat cgcggccggc
    31861 ggcatcaaca gccatgagaa ggtgcgggcc gtcctcggcc tgggcgcctc cgcagcccag
    31921 ctcggcacgg cgttcgcggt gaccgaggag ggcgacgccc accccgtgtt caagaaggtg
    31981 ctggccgacg cgcggcccga ggacatcgtc actttcatga gcgtggccgg cctgccggcg
    32041 cgtgccgtgc gcacgccctg gctcgaccgg tacctgcgcc gcgaggagcg catgaaaggc
    32101 aaggcgcgcc ccaagcccca gtgcacgctc gcctgggact gcctgctgca ctgcggcctg
    32161 cgcgacggac tggcgcgcgc cgggcagttc tgcatcgaca accagctggg cgccgcgctg
    32221 cgcggcgacc tggaacgcgg cctgttcttc cgcggttcgg agcccttgcc cttcggctcg
    32281 gcgatccgct ccgcggcgga cctgatcggc tacctgatga gcggcgtcta ccctccgggg
    32341 gtgcagccgc gctgagacgg ctcgcatgga cggcgcgcga cttcccgggt agcatcggcc
    32401 gccgtgaatc ggccttgtgt cgtgcgcatc gtcgcgctgc tgctggtgct ggtcggcctg
    32461 caggccggcg cgttccgcga cctggacacc gggcacgaat ggtacttggg cgccgaagac
    32521 aacagcgccg cggtgatggc cgagtcgcgc gaggccgatc gcccgatcac cggtgtgccg
    32581 gcggcgcatg cgccgtcggc gctgcccgcg ccggtaggcg ccgcactcga gatcgacgct
    32641 gcgccaggaa gcgccggccc gtccgcaccg cgcgccttcc ggccccacgc caccggcccg
    32701 cctcgggcct gactccgcaa ctcggccagt agttcggtca accgcgacgc cgcgcgcctg
    32761 agcgcgccgc atcgccactg gagtccacca tggacaccct gcagcaacag gccggcgcgc
    32821 gccatgcggt gctgcaccgg ctgatcttcg gtatcgactt cctgagcagg acggtcggcc
    32881 acagcttcgc ctggtgcatc gccatcctca cgcttggcac ctgcttcgag gtgatggtgc
    32941 gctacgcgct gaacgacccg acgagctggg ccttcgacat gagctacctc atgtacgcgg
    33001 gggtgttcta catggccggc gcctacacgc tgtcgcgcgc cgcgcacgtg cgcgccgaca
    33061 tgctctaccg gctgtggcgg ccgcgcgtgc aggcggcggt cgagttcacg ctgtacgtgt
    33121 tcttcttctt tcccggcatt ctcgcgctgg tgatcgccgg ctggggctac ggcaccgagt
    33181 cgacgcgcat cctcgagtcg agcgtgaaca gccccgccgg cgtgccgatc tggccgctga
    33241 agatgatgat cccgctgggc gccgggctgc tcgcgctgca aggccttgcg gagctgttgc
    33301 gctcctgggt ctgcctgcgc gagggcgagt ggccgccgcg cctgcacgac gtcgaggagc
    33361 tcgagaagca gatcctgatg aaggcggccg acatgcgcga gaaggggggc gccctatgag
    33421 cgatcccgtc gttgcgctcg tcatgctggg gatcctggtg tcgacgatca tgatcggctt
    33481 cccggttgcc ttcaccctga tggcgctcgg ggtagggttc ggctactacg cgtacttcca
    33541 gcccggccag gcattcttcg acaaccgcgt gttctacctg ctcacgcaga acaccttcac
    33601 ggtaatgaac aacgatgccc tggtgtcgat cccgctgttc ctcctgatgg ggtacatggt
    33661 cgagcgggcg aacatcctcg attcgctgtt cggcagcttg caggtggcat tgcgcgccgt
    33721 gccgggctcg ctcgccgtgg cgacgctcgc cacctgcgcg cttttcgcta ccgccacggg
    33781 catcgttggc gcggtggtga cgctgatggg tctgctcgcg ttcccgcaga tgctgaaggc
    33841 gggctacgac acgcggcttg ccgccggcgt ggtgtgcgcg ggcggctgtc tcggcatcct
    33901 catcccgccc tccatcatgc ttatcgtgta cggcgctcag gcgagcatct cggtggtaaa
    33961 gctctacgcc gcggcgctcc tgcccggatt cctgcttgcc ggcctgtaca tcgtctacgt
    34021 gatcggcctc gcggtcttca agccggccgc ggcgccggcg ctgccgaagg aaaggaccga
    34081 catcgctttc ggccaggtgc tgctgatgct ggcgaagtcg ttcttcccgc tcgccatcct
    34141 catcctgtcg gtgctgggcg cgatcatgtt cggcatcgcg acgcccaccg aggcggcggc
    34201 ggtcggcgcc ttcggcgccg tggtgctggc tgcgggctat cgctcgctca cctggcaggg
    34261 catgcgcgag gcggtgtacc tcaccgcgcg tacctcggcg atggtgtgct ggctgttcgt
    34321 cggctcggcg acgttcgcct cggtgttcgc gtacctcggc ggcgagggcc tgatcaagtc
    34381 gacggtgctc ggcatgaacc tgagcccgat ctccttcctg ctgctgtcgc agttcatcat
    34441 cttcctgctc ggctggccgc tggagtggac cgagatcatc atcatcttcg tgccgatctt
    34501 cctgccgatg ctgccgcact tccagatcga tcctctcctc ttcggcgtgc tggtcgcgct
    34561 caacctgcag accgcgttcc tctcgccgcc gatggcgatg tccgcgtact acctgaaggg
    34621 cgtggcaccc aagcacgtgc tgctcacgca gatcttcggc ggctgcatgc cgttcctcta
    34681 catcgtcatc gtctgcatgg cgatcgtcta catctttccc gacgtggccc tgtggctgcc
    34741 gcgggcactg tacggttgac cgacaacaag gagaaacgcg cgtcatggaa accctgagca
    34801 ccgccgtact cgtcttcgtg gtgagcgcgg ccttcgtgat cgccgcgggc ataggcctcg
    34861 cgcgctacgg cgacgacctc gcggagtcca ccggctgggg gaaactctgg gtggggacgc
    34921 tgctcgtcgg catcgccacc tcgctccccg aagtcaccgt caacatctcc gcggtgtggc
    34981 tcgaggattc gcccgggctc gcgctcggca acgtgttcgg cgccgacatg atgaacgtgt
    35041 tcgtgctggg ctgcgtgggc cttgccttcg gcatcggcaa cctgttcggc gggcaagggc
    35101 gcgacaccga gctgctgatc ctggtcggca tggcgctggt cgcgctcgcc ctcgtcttcg
    35161 gcatcgcggg cgacgtgaag ctcgggccga cgagcctcgg cacggcgctc atcctcgccg
    35221 cctacctcgt cggcatgcgc atggtgtaca aggccggcca gacgcacatg cacctggagg
    35281 acatcccggc gcccaccggc agcgcgaaga aggcctggat cggcttcggc atctcggtgg
    35341 cggtggtgat cgtcgccggg cgcttcctcg ccgcgagcgc cgaccgcatc gccgagatct
    35401 ccggcatcag cgcgagcttc atcggcgtgc tgctcgtatc gatcgttacc acgctgcccg
    35461 agggctcggt gacggtggcg gcgacccttc gcaagtccta cggcattgcc atgggcaacg
    35521 tctacggctc gaacgccttc aacgtcacca tcctgacgtt ctcggacctg ttcagcccgg
    35581 cgccgctcct tggcaagatg gagggcgcgc acttcgcggc gggtctcgca gcgttcgtcc
    35641 tgatgctgat tggctacctg gtgtggaagg gggtgcagcg accggccttc gcctgggcgc
    35701 ggacgctcgc gccggcgata ccggtcgtct acgtcggcgc cctgtacatc gtttacacgc
    35761 tggcgcaagc ctgacgcttt gctatgatgc cagcagggtc tttgccctgg gggggaaaga
    35821 aaatgctggc ggtatggaga ctggcgtcgg ccgcgctcgg cctggcggcg gcggcgctcg
    35881 ttcaggcgca gggttcggac gagctgtgga acatgtctgt tcggatggag atggacggca
    35941 tgcagatgcc ggcgatgagc cagcaggtat gccagaagaa aggccagccg ggcgccgcgc
    36001 cggcgccgga cccgaactgc acgacctcgg acgtgcgcac cgtcggcaac aagacgacct
    36061 ggaagatggc ctgcaccggc gacgagccga tgagcggcac gggcgagatc acgcgtacgc
    36121 gcgacagcat gagcgggcgc atgcagctgc gcggcaagga cggcttgacg atgaacgtcg
    36181 tctactcggg cacgctcgcg ggcacctgca atgcgcagac gcaccaggat ccgcagcagg
    36241 cggcgacgaa gcagaagatg gcggcgatgc aggcgcagag caatgcgcag ctggcgcaga
    36301 cctgccgcga gtccatcgag aagttcgcga cgacgctgtt cgagatgcag ggcaatccct
    36361 gcgccgcgag cacgggcgag tactgtgcgc atgtgaagaa gaccgcgcag tcaatgcgca
    36421 cgccggcagg ctaccgccag gcgatgcagg tcgagggcct gcgcaacgac ggctttgagc
    36481 gggcggcgca attctgcaag gtggaccctg cgccggtgcg cgcggcggcg tgcaagagcg
    36541 cggtcgacgg ccgcgactgg ccattcgtca ccgagtactg cccggcggag tcgcagaccc
    36601 tcgccaagca gcactgcgcc gggcgtgaat acacggtcgt catgaagacg gagtacggcc
    36661 cgctgtgctc caagcactcg aacctgctcg ccaaaggtcc ctgcggcggc gggcgctcgt
    36721 acaccgtggt aaaggccggg ggaggatggg acacgaaatg cggcgagcgg cccgcgcagg
    36781 ttgcgacacc gcccgccgcg ccgagcgccg gcgacgcggt caaggaaggc gtgcgcgggc
    36841 tgcgcaagct gttcggaaac tgaccgcggt ggcgtggcgc ctcgcgctcg cggcgctgct
    36901 cgcgctggcg gggccggttg cccacgcggc gccggaggac ccctttccgc ggatcgcggc
    36961 ggcctacctg gtgcttgtcg acggcgagcc gctctgggcg cacgccccgg acgcgcggcg
    37021 cgcgcccgcg agcctcgcca agctcatgtc ggcgctggtc gtcgtcgagc aggacgcgct
    37081 cgacgaggtc gtgacgatcg gcgccgaggc cgcgcgtgcg cgcggctcgc gcctgggact
    37141 gcacgccggg gagcgggtgc cggcgcacgg cctgctggcg gcaatgctcg tcggctcggc
    37201 caacgatgcc tgctacgcgc tcgccgcgcg gcgcgggcgc gtgagcgact tcgtcgcgcg
    37261 catgaacgcg cgcgccgccg agctcggact gcgcgccacg cgcttcggcg atccctgcgg
    37321 tttcgaccgt gaccggcagt acagcacggc gcgcgatctg gcgcgtctcg cgcaggcctt
    37381 catgcagcgg gcgcaactcg cggcgctggt gggccagctg cgggtggagg tggcaaccga
    37441 ggggcgcggg cgcgtgttca cggtgaaaaa taccaacgtg ctggtggaat tctttcccgg
    37501 gacgatcggc gtgaagaccg g
//
LOCUS       DBNHKE010000121        14356 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_126256, whole genome shotgun sequence.
ACCESSION   DBNHKE010000121 DBNHKE010000000
VERSION     DBNHKE010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14356
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_126256"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="AC16O9_07620"
     CDS             complement(<1..435)
                     /locus_tag="AC16O9_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PmbA/TldA family metallopeptidase"
                     /protein_id="HIR6516522.1"
                     /translation="MDIDQLLATADACLLAPYDLQARRLDDVFGALAAPRMDHADLYF
                     QYTRAEGWSLEEGIVKAGSFSIEQGVGVRAMSGERTAFAYSDEISFDALQDAARATRA
                     IARQGGGRAARIARRASAPRRYAPLDPLASLDSAAKVALLEKL"
     gene            complement(445..4269)
                     /locus_tag="AC16O9_07625"
     CDS             complement(445..4269)
                     /locus_tag="AC16O9_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIR6516523.1"
                     /translation="MQSSRQRTAAGRLLRALEILAWTAFFAFAGVFLALRFWLLPQVE
                     RYQGHVVAALTRAVGLPVTIGALRADWEGLHPRLEVSDLRVYDRDGREALVLPSVEPV
                     VGWASLLAGDLRLYSLTIEGPRLTVRRGADGVISVAGISLGARGGGGGGDGRFADWIL
                     EQREIIIRNAEIEWIDEWRGAPPLALHSLQFRLRNRGEVHQFGLSARPPPALGAGMEL
                     RASLIGRSVAVPAAWNGRVYAELGYTDLAGWRAWIDYPVEVRSGQGALRLWATFGAGK
                     LVDATADLALSGVAARLGEDLPELRLASVAGRVQGRGTEHGYEFGTRRLALVPVDGQP
                     MHGTTFRASWEATEPAHGTLSADLLELEPLARLAEYLPFPADLRALLAELAPRGRVSD
                     ASFEWHGRLPSDARFKGRARFDGMSMSAWRAIPGFANLSGRIDATESRGVLVLATQGG
                     EVALPRLFPEPRIRLASLSGEVSWERPTPGGARVRIAGLQFANADLAGSASGSYAYAG
                     EGPGTIDLRAQLQRFDARSLPQYLPRAELMGPRTRAWLVDAIRGGQSADVRLRLQGNL
                     RHFPFADPALGQFQVVAQVRDGVLAYAEGWPAIEGIEGELRFERERVAMLARRARVFG
                     ATLQDTRAGMGLRAPAVLEITGQAEGPSADFLEFMRRSPLRSLLGEADEAVRVEGRGR
                     LRLGLQIPLAERDKTTASGQYEFAGNSLRIGKTLPPIERAAGTLAFTESSVQVRNASG
                     RFLGGPLRVIGGTQRGGGVLLSANGQFTVEGLQPLVPEPWHGRLQGASSYAGSVRLAR
                     GAAPQIVLESHLAGVSSDLPPPLAKEADRILLLRVSFLQGEGDERERVSVALGRLLRA
                     EVLQQRDGEELVERTAVAFHPPPGARLRFPERPARTLLYGSLQEFDLDGWRALFGEAA
                     SRGEGRTAVDMSVGTLDAFGKRMQDITVKAQLDGGGWTATLNSKDIAGEVAYRTGDQA
                     KLQARMARFDVPPDVPGSAPARASRELPAVDLVAEDFGFRGKRFGRVEIVAQREGADW
                     RVERLSMQNPEGSVRGEGLWRVAPAPRTTFDFEMQSSDVGRFLDRVGFPGLVRGGKAN
                     AKAALAWDGEPAAIDYASLSGSLELHAEDGRFLEIEPGIGKLVSLMSLQMLPRRIALD
                     FRDVFSKGFQWDNIDATAQIAQGTLQTENFRMSGGAAEVRMSGTVDLAAETQELHVRV
                     VPALDSTASTAAAVAINPVVGLTTLLAQKLLQNPLGQMFAFEYGISGSWTDPKVEKLT
                     PMPVPPKPPVGD"
     gene            4399..6966
                     /gene="glnE"
                     /locus_tag="AC16O9_07630"
     CDS             4399..6966
                     /gene="glnE"
                     /locus_tag="AC16O9_07630"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR6516524.1"
                     /translation="MPALDGKLSFSRYAARSLAARPELAAALDAPARCTREEIAAALR
                     GAEGDDEPALKRRLRRLRQEVLLRVMARDLGGSADLAEVCATMSDLAELQIAAALDWT
                     RAPDLVVVAMGKLGGRELNVSSDVDLVFAYPAGPDPEPLERAGRKLIALLSEITDDGF
                     AFRVDMRLRPYGDSGPLASSYEALEAYFVAQGREWERYAWIKARPITGSRHDELAGIV
                     HPFVYRKYLDYPTLAAMRRLHAEVRRDVARRELAEHVKLGPGGIREIEFIVQALQLVR
                     GGRDPALAVRQTLAALARLGERQLLPADAVRELAAAYVFLRNTEHRLQYLEDQQTHTL
                     PEQAEDRERIARMSDCASWDEFYGRLQAHREAVQRHFQDVFAESAAVDAPDLWQDDRA
                     AMAQALSGRGFRNPDMSADRLVAARRGQRYAVLPTDSRRRFDALVPALAAAAAHTADP
                     DATLGRGLELIETIARRAAYLALLAEHPEALERVTKMIAASSWAAEFVTRHPLLLDEL
                     LDDRMLYAPPDWPVFERTLTALLVDAAGDVELQMNVLREQHQALVFRLLAQDLAGLLT
                     VERLADHLSQLADLVLGAALDVVWAQLDAHHREAPQFAVIAYGKLGGKELGYASDLDL
                     IFLYDDADERAPENYARLAQRLNHWLTTRTSSGVLFETDLRLRPSGESGLLVISVDAF
                     REYQERSAWVWEHQALTRARYCAGEPRVGAAFESLRTEILERARDPAALAREVIRRRE
                     RMHEAHPNRSGLFDVKHDRGGMIDVEFVVQYLVLAHAHRHRDLTGNLGNIALLGMAAG
                     HGLVAPELARECQDAYREFRRLQHGLRLNGAQFARVPPDKVSAQVRAVRALWARVFD"
     gene            complement(6963..7745)
                     /locus_tag="AC16O9_07635"
     CDS             complement(6963..7745)
                     /locus_tag="AC16O9_07635"
                     /inference="COORDINATES: protein motif:HMM:NF022694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HIR6516525.1"
                     /translation="MRPAAVLAALLALGGCAVVPEDDGLLASTPIVRELERHAGDVVE
                     VARFSRLRVGAPLPADWVGWGLHSGKRHTDYRLVNGSAGTVLQATAERAATGLYRRVR
                     VDPFRQGLLEWSWRVDDLMPGTDKRVASREDSVARLVVSFHGDPERLDFQQRALLRLA
                     RVFAGEPLPYAMLIYVWSNRIPVETALQSPQIERIRMIVVESGAARLGHWLRYRRNVL
                     EDYRRAFGEDPGDIVAVGVLTDSDNTQQTARALYGDITLRAP"
     gene            complement(7745..8047)
                     /locus_tag="AC16O9_07640"
     CDS             complement(7745..8047)
                     /locus_tag="AC16O9_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6516526.1"
                     /translation="MDANPQDEVFAALASLPRRRALAFLAQTALSTTDLAERFGMSPP
                     AVSRHLAVLQRAGLVSAERQGQRVLYRLNRDALLEALGGLASAVDGPLRRAPREAI"
     gene            8116..9090
                     /locus_tag="AC16O9_07645"
     CDS             8116..9090
                     /locus_tag="AC16O9_07645"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR6516527.1"
                     /translation="MAKAKAAPKGPLSMADRDGWIWHDGKLVPWRSATTHVLTHSLHY
                     GLAVFEGVRAYKTVRGTAIYRLKEHTQRLINSAHIYMMKIPYTLEQLMKAQIEVVRAN
                     GHEACYVRPIAFYGSEKMGVSPVGARVHVAIAAWPWGAYLGPEALEKGIRVKTSSYAR
                     HHINVTMARSKMSGTYPNSVLATLEATMHGYDEGLLLDVDGFVAEGAGENIFIVKDGV
                     IYEPELTSALTGITRASVIELAAGLGYKVESRRLTRDDIYIADECFFTGTAADVTPIR
                     ELDSRVIGAGKAGPITKKLQKAFFDVVTGKDKKHTHWLTPVPMRKGKP"
     gene            9087..9278
                     /locus_tag="AC16O9_07650"
     CDS             9087..9278
                     /locus_tag="AC16O9_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIR6516528.1"
                     /translation="MSTDTARQVDVTEKDLPLHCPLPGAPLWARHPRVFLDVAKAGQA
                     LCPYCGTRYVYKGAAPRGH"
     gene            9282..10247
                     /gene="waaF"
                     /locus_tag="AC16O9_07655"
     CDS             9282..10247
                     /gene="waaF"
                     /locus_tag="AC16O9_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR6516529.1"
                     /translation="MARLLVVAPNWIGDALMAQPLLSRLRENQPQARIDVVAPAWVAP
                     VFRRMPEVAGVIETPFAHGALHLRERWRLGRTLKALGYDEAYVLPNAWKAALVPFFAG
                     IARRCGYRGESRYGLLNVVHRKQPGREPMALHYARLAGAPGAPVAQPLPDPKLAVDAP
                     AVRAARERFGLPERYAVLCPGAEYGPAKRWPYFPELAARIDTPVVVLGSAKERELCAT
                     VGGRNLAGETTLDEALDVLAGAARVVSNDSGLMHVAAALGRPLVAVFGSSSPRHTPPL
                     SPRARVLWLGIECSPCYARDCPLGHFRCMREMTVERVMQEMQQLP"
     gene            10251..10679
                     /locus_tag="AC16O9_07660"
     CDS             10251..10679
                     /locus_tag="AC16O9_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR6516530.1"
                     /translation="MPLRIFPTPQDAESAFYEALERADLELMMAVWSEDEEIVCVHPG
                     GPRLAGQDQVRDSWRQIFAGGASARVHVTQQVTVTGTMVAVHSVHENFSRPGQARPVQ
                     AVVATNVYLRTAAGWRMVAHHGSPAPGSAEPPPPAPKILH"
     gene            10676..11596
                     /locus_tag="AC16O9_07665"
     CDS             10676..11596
                     /locus_tag="AC16O9_07665"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR6516531.1"
                     /translation="MSAYHAPWWLPGGHLQTVYSALGPAPRIAWRRERWDTPDGDFID
                     LDWAEGGRGPLLALFHGLEGGSSSHYARKVAAQAQRRGWRCVVPHFRGCSGEANRHAR
                     AYHSGDSAELDWILRRLRPDFAAGVSLGGNVLRKWLGEQGAAAAQLVRRAAAVSAPVD
                     LAASGHALDRTLNRWLYAAHFLTTLKPKALAKISQHALAIDATRVSRARTLHEFDDLV
                     TAPLHGFRDADDYWTRAASGPWLERIRVPTLVLNAMNDPFLPHAALAAAARRASPDVV
                     LEFPATGGHAGFPGRGHWLARRVLEFCAAP"
     gene            11593..12951
                     /locus_tag="AC16O9_07670"
     CDS             11593..12951
                     /locus_tag="AC16O9_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR6516532.1"
                     /translation="MTIAAEIFRAYDIRGIAGKTLTVDAVRGIGRALGSMADEFAVGR
                     DGRDSGPALVNALCEGLQRSGASVIDIGMAPTPVTYFAAHQLNCGSCVSVTGSHNPPE
                     YNGLKMVVDGHTLSGDEIQGLRRRIERGEFRAGAGRRREADVLDAYVERITGDVRLAR
                     RMRIATDCGNGVAGMVAPRLYRALGCEVRDLYSDVDPTFPNHHPDPSHTENLADLIAA
                     VRDGGAELGLAFDGDGDRLGVVTRDGSVIYPDRQLMLFAADVLSRNPGAEIIYDVKST
                     RLLAPWIERHGGKPLLWKTGHSLIKAKLAETGAPLAGEMSGHTFFKERWYGFDDALYA
                     GARLLEILSRSHDASAVLTSLPDAPSTPELQWKLAEGEPHALIAKLQAAKPFPKAART
                     ITIDGVRAEYADGFGLARASNTTPVIVLRFEADTKAALARIQDEFRAALQPLKPGAPL
                     PY"
     gene            complement(12948..13688)
                     /gene="ugpQ"
                     /locus_tag="AC16O9_07675"
     CDS             complement(12948..13688)
                     /gene="ugpQ"
                     /locus_tag="AC16O9_07675"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6516533.1"
                     /translation="MSHWPYPRIVAHRGGGTLAPENTLGAIRLGAEMGFKGVEFDVML
                     AGDGTPVLIHDETLRRTTGAHGYVAQTPYAELQRLDAGAWRGARWRGEKIPRFEDAVR
                     LCRHLGLWANVEIKPGRGYERRTGETVARMAAELWRGAALPPVLSSFSPVALAAARQA
                     APELPRGLLLGRLLPHWREMLRDLECVALHANFRSLRQRVVQEAHAAGCAVLAWTVND
                     RRAARKLLGWNVDCLVSDRLDRIGPDFA"
     gene            complement(13685..14149)
                     /locus_tag="AC16O9_07680"
     CDS             complement(13685..14149)
                     /locus_tag="AC16O9_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HIR6516534.1"
                     /translation="MKFSARTLVRGMHWWGPFRSPGIRLRRMTDDFREAEAELRLGWL
                     NRNIAGAQFGGSLFAMTDAFYMLMLMQNLGPQYYVWDKSASIEYLAPARGTVTARFSL
                     TEEHLAKIRAQAAGGEKVLPEFVTDVVDAQRAVVARVHRTLYVRLKPEHRPA"
     gene            complement(14154..>14356)
                     /locus_tag="AC16O9_07685"
     CDS             complement(14154..>14356)
                     /locus_tag="AC16O9_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HIR6516535.1"
                     /translation="ITLEVDLIEPLGADTLVYGHFGDGEGARVAARLHASVDARAGKL
                     PLRYDPAQVHWFDPQSGKRIEG"
BASE COUNT         2046 a         5284 c         5057 g         1969 t
ORIGIN      
        1 cagcttctcc agcagcgcca ccttggccgc cgaatcgagc gaggcgagcg gatcgagcgg
       61 cgcgtagcgg cgcggcgccg aggcgcggcg cgcgatgcgc gcggcgcggc cgccgccctg
      121 gcgcgcgatg gcgcgcgtgg cgcgcgccgc gtcctgcagc gcgtcgaagc tgatctcgtc
      181 cgagtaggcg aacgcggtgc gctcgcccga catggcgcgc accccgacgc cctgctcgat
      241 cgagaagctg cccgccttga cgatgccttc ctcgaggctc cagccttccg cgcgcgtgta
      301 ctggaagtac aggtcggcat ggtccatgcg cggcgcggcc agcgcgccga acacgtcgtc
      361 gaggcggcgc gcctgcaggt cgtagggggc gagcaggcag gcgtccgcgg tagccagcag
      421 ttgatcgata tccatgcgcc tccgtcagtc tcccaccggc ggcttgggcg gcaccggcat
      481 gggggtgagc ttctccacct tcgggtccgt ccagctgccg ctgattccgt actcgaaggc
      541 gaacatctgg ccgagcgggt tctgcagcag cttctgcgcc agcagtgtcg tcagacccac
      601 caccgggttg atcgccacgg ccgccgccgt cgatgcggtg ctgtccagcg ccggcaccac
      661 gcgcacgtgc agctcctggg tctccgcggc gaggtcgacg gtacccgaca tgcgcacctc
      721 ggccgcgccg ccgctcatcc tgaaattctc ggtctgcaga gtgccctgtg caatctgtgc
      781 cgtcgcgtcg atattgtccc actggaagcc cttggagaac acgtcgcgga aatcgagcgc
      841 gatgcgccgc ggcagcatct gcaggctcat cagcgacacc agcttgccga tgccgggctc
      901 gatctcgaga aagcggccgt cctccgcgtg cagctccagg ctgccggaca ggctcgcgta
      961 gtcgatcgcg gccggctcgc cgtcccaggc gagcgcggcc ttggcgttcg ccttgccgcc
     1021 gcgcaccagg cccgggaagc ccacgcgatc gaggaagcgt ccgacgtcgc tcgattgcat
     1081 ctcgaagtcg aatgtggtgc gcggcgccgg cgcaacgcgc cacaggccct cgccgcggac
     1141 cgagccctcc gggttctgca tcgacagccg ttccacgcgc caatcggccc cctcgcgctg
     1201 cgcgacgatc tccacgcgcc cgaagcgctt gccgcgaaaa ccgaaatcct cggccaccag
     1261 gtccaccgcc ggcagctcgc gcgaggcgcg ggccggcgcg ctccccggga cgtcaggcgg
     1321 cacgtcgaag cgcgccatgc gcgcctgcag cttcgcctgg tcgccggtgc ggtacgcgac
     1381 ctcgccggcg atgtccttgg agttcaatgt cgcggtccag ccccctccgt ccagctgcgc
     1441 tttcaccgtg atgtcctgca tgcgcttgcc gaatgcatcg agcgtgccga cgctcatgtc
     1501 aaccgcggtg cgcccctcgc cccggctcgc cgcctcaccg aatagcgcac gccagccgtc
     1561 gaggtcgaac tcctgcagcg agccgtagag gagcgtgcgc gccgggcgct cgggaaagcg
     1621 cagccgcgcc cccggcggcg gatggaaggc gactgccgtg cgctcgacca gttcttcacc
     1681 gtcgcgctgc tgcaggacct cggcgcgcaa caggcgccca agcgccacgc tgacacgctc
     1741 gcgctcgtcg ccttcgccct gcaggaagga cacgcgcagc agcaggatgc ggtcggcctc
     1801 cttcgccagc ggcggcggca ggtcgctcga aacgccggcc agatgcgatt cgagcacgat
     1861 ctgcggcgcc gcgccgcgcg ccaggcgcac cgagccggcg taggaggagg cgccctgcag
     1921 acggccgtgc cacggctcgg ggaccagcgg ctgcaacccc tccacggtga actggccgtt
     1981 cgcgctgagc agcacgccgc cgccgcgctg cgtgccgccg atgacgcgca gcggcccgcc
     2041 gaggaagcgg ccgctggcat tgcgcacctg gacggaggat tcggtgaacg ccagcgtgcc
     2101 cgcggcgcgc tcgatcggcg gcagggtctt gccgatgcgc agggaattgc cggcgaactc
     2161 gtactggccg gacgccgtcg tcttgtcgcg ctcggcgagc gggatctgca gcccgagccg
     2221 caaccgcccg cgtccttcga cgcgcacggc ttcgtccgcc tcgcccagca ggctgcgcag
     2281 cgggctgcgc cgcatgaact cgaggaagtc ggcgctcggc ccctcggcct gccccgtgat
     2341 ctcgagcacc gcgggtgcac gcaggcccat cccggcgcgc gtgtcttgca gcgtggcgcc
     2401 gaacacgcgt gcgcggcgcg cgagcatcgc cacccgctcg cgctcgaagc gcaactcgcc
     2461 ctcgatgccc tcgatcgcgg gccagccttc ggcgtaggcg agcacgccgt cgcgcacctg
     2521 cgccacgacc tggaactgcc cgagcgcggg atcggcgaac gggaagtggc gcaggttgcc
     2581 ctgcagccgc agccgcacgt cggccgactg accgccgcgg atcgcgtcca ccagccaggc
     2641 gcgtgttctc gggcccatca gctcggcccg cggcaggtac tgcggcaggc tgcgcgcgtc
     2701 gaagcgctgc agctgcgcgc gcaggtcgat cgtgccgggc ccttctcccg cataggcata
     2761 gctgcccgac gcgctaccgg cgagatccgc attggcgaac tgcagcccgg cgatgcgcac
     2821 gcgtgcgccg cccggcgtgg gtcgctccca gctcacttca ccgctgagcg aggcgaggcg
     2881 gatgcgcggc tcggggaaca ggcgcggcaa ggccacctcg ccaccctgcg tggcgaggac
     2941 cagcacgccc ctggactcgg tggcatcgat gcgccccgaa aggttggcga acccagggat
     3001 cgcgcgccac gcgctcatgc tcatgccgtc gaaccgcgcg cggcccttga agcgggcgtc
     3061 gctcggaagc cgtccgtgcc actcgaagct cgcgtcgctg acgcggcccc gcggcgcaag
     3121 ctcggcgagc agcgcgcgta agtcggccgg aaacggcagg tactccgcga gccgcgccag
     3181 cggctcgagc tcgagcagat cggcgctcag cgtgccatgc gccggctcgg tcgcctccca
     3241 gctggcgcgg aatgtggtgc cgtgcatggg ttgcccgtcc accgggacga gcgccaggcg
     3301 ccgcgtgccg aactcatagc cgtgctcggt accgcggccc tgcacgcgcc cggcgacact
     3361 cgccaagcgc agctccggca ggtcttcgcc cagtcgcgcg gcgacgccgg aaagcgccag
     3421 atcggcggtg gcgtccacca gcttgcccgc gccgaacgtc gcccatagcc gcaacgcccc
     3481 ttggccgctg cgcacctcga ccgggtagtc gatccaggcg cgccagccgg cgaggtccgt
     3541 gtatccgagc tccgcataca cgcggccgtt ccaggccgcg ggcacggcca cgctgcggcc
     3601 gatgagcgaa gcgcgcaact ccatgccggc gccgagtgcc ggcggcggcc gcgccgacag
     3661 gccgaactgg tgcacctcgc cgcggttgcg cagccggaac tgcaggctgt gcaacgcgag
     3721 cggcggtgcc ccgcgccact cgtcgatcca ttcgatctcg gcgttgcgga tgatgatctc
     3781 gcgctgctcc aggatccagt cggcgaagcg gccgtcgccg ccaccgccgc caccgcgcgc
     3841 gcccagggag atgccggcca ccgagatcac gccgtcggcg ccgcgacgca cggtgaggcg
     3901 cgggccctcg atggtgagcg agtacaggcg caggtcgccc gccagcaggc tcgcccagcc
     3961 caccacgggc tccaccgacg gcagcaccag cgcttcgcgc ccgtcgcggt cgtacacgcg
     4021 caggtcgctc acctccaggc gcgggtgcaa tccttcccaa tcggcgcgca gcgcgccgat
     4081 ggtcaccggc aagcccaccg cgcgcgtcag cgcggcgacc acgtgcccct ggtagcgctc
     4141 gacctgcggc aggagccaga agcgaagcgc gagaaatacc ccggcaaaag cgaagaaagc
     4201 cgtccaggcc aggatttcca gcgcgcgcag cagcctgccc gccgccgtgc gctgcctcga
     4261 ggattgcatt gcgcttcgcg ttgcccctat agtcgtagcc gctcgcggcc tgaattacgc
     4321 ccggcgtctt gattgggggc ggcgcggcgc gaaaagttct cgcgcggatg tcgttcccac
     4381 gcatcattct aaccgcagat gcccgcactc gacggcaagc tttccttctc gcgctacgcc
     4441 gcgcgcagcc tcgccgcgcg cccggagctg gctgcggcgc tggacgcgcc ggcacgctgc
     4501 acgcgcgagg agatcgccgc agcgctccgg ggcgccgaag gcgacgacga gccggcgctc
     4561 aagcgccggc tgcgccgcct gcgccaggaa gtgctgctgc gcgtgatggc gcgcgacctg
     4621 ggaggcagcg ccgatctggc ggaagtgtgc gccacgatga gcgatctggc cgagctgcag
     4681 atcgcggcgg cgctcgactg gacgcgcgcc ccggacctgg tggtggtggc gatgggcaag
     4741 ctcggcgggc gcgagctgaa cgtctcctcc gacgtcgatc tggtcttcgc ctacccggcc
     4801 ggcccggacc ccgaaccgct cgagcgcgcg gggcgcaagc tgattgcact gctctcggag
     4861 atcaccgacg acggctttgc cttccgcgtc gacatgcgcc tgcggcccta cggcgactcc
     4921 gggccgctcg cgtcgagtta cgaggccctg gaggcctact tcgtggcgca gggccgggag
     4981 tgggagcgct acgcgtggat caaggcgcgg ccgatcactg gctcgcgcca cgacgagctc
     5041 gccggcatcg tgcacccctt cgtgtaccgc aagtacctcg attacccgac gctcgcggcg
     5101 atgcgccggc tgcacgccga agtgcggcgc gacgtggcgc ggcgcgagct cgccgagcac
     5161 gtcaagctcg gccccggcgg catccgcgag atcgagttca tcgtgcaggc gctgcagctg
     5221 gtgcgcggcg ggcgcgatcc ggcgctcgcg gtgcgccaga ccctggcagc gctggcgcgg
     5281 ctcggcgagc ggcagctcct gcccgccgac gcggtgcgcg agctcgccgc ggcctacgtg
     5341 ttcctgcgca ataccgagca ccggctgcag tacctcgagg accagcagac gcacacgctg
     5401 ccggagcagg ccgaggaccg cgagcgcatc gcccgcatgt cggactgcgc ctcctgggac
     5461 gagttctacg gccggctgca ggcgcaccgc gaggcggtgc agcggcattt ccaggacgtg
     5521 ttcgcggagt cggcggcggt ggacgcgccc gacctgtggc aggacgaccg cgcggcgatg
     5581 gcccaggcgc tgtcgggccg tggcttccgc aaccccgaca tgagcgctga ccggctcgtt
     5641 gcggcgcgcc gcggccagcg ctacgcggtg ctgcccacgg actcgcggcg tcgcttcgac
     5701 gcgctggtgc ctgcgcttgc tgccgcggcg gcgcacaccg ccgatcccga tgccacgctg
     5761 gggcgcggcc tcgagctgat cgagaccatc gcacggcgtg ccgcctacct ggcgctgctt
     5821 gcggagcacc ccgaggcgct ggagcgcgtc accaagatga tcgccgcctc gagctgggca
     5881 gcggaattcg tcacgcgcca cccgctgctg ctcgacgagc tgctcgacga tcgcatgctg
     5941 tacgcgccgc ccgactggcc cgtcttcgag cgcacgctga ccgcgctgct cgtcgacgcc
     6001 gcgggcgacg tcgagctcca gatgaacgtg ctgcgcgagc agcaccaggc gctggtgttc
     6061 cggctgctcg cgcaggacct tgccggcctg ctgacggtgg agcgcctcgc cgaccacctg
     6121 tcgcagctcg ccgatctcgt gctcggggcg gcgctcgacg tggtctgggc gcagctcgac
     6181 gcgcaccacc gcgaggcgcc gcagttcgcg gtgatcgcct acggcaagct cggcggcaag
     6241 gagctcggct acgcctccga cctcgatctc atcttcctgt acgacgacgc cgacgagcgc
     6301 gcgcccgaga actatgcgcg cctcgcgcag cgcctgaacc actggctcac cacgcgcacc
     6361 tcgagcggcg tgctgttcga gaccgacctg cggctccggc cctccggcga atcggggctg
     6421 ctggtgatct cggtcgacgc gtttcgcgag tatcaggagc gctccgcctg ggtctgggag
     6481 caccaggcgc tgacgcgcgc gcgctactgc gccggcgagc cgcgggtggg cgcggcgttc
     6541 gagtcgctgc gcacggagat cctcgagcgg gcgcgcgatc cggccgcgct ggcgcgagag
     6601 gtgatccgca ggcgcgagcg catgcacgag gcgcacccca accgatccgg gctgttcgac
     6661 gtcaagcacg accgcggcgg catgatcgac gtcgagttcg tcgtgcagta cctggtgctg
     6721 gcgcatgcgc accgccatcg cgacctgacc ggcaacctgg gcaacatcgc gctgctcggc
     6781 atggcggccg gccacggcct ggtggcgccg gagctggcgc gcgaatgcca ggacgcctac
     6841 cgcgagttcc gccggctgca gcacgggctg cgcctgaacg gcgcacagtt cgcgcgcgtg
     6901 ccgccggaca aggtgtcggc gcaggtgcgt gcggtgcgcg cgctgtgggc gcgcgtgttc
     6961 gactagggcg cgcgcagcgt gatgtcgccg tacagcgcgc gcgccgtctg ctgcgtgttg
     7021 tcggagtcgg tgagcacgcc cacggcgacg atgtcgccgg gatcctcgcc gaaggcgcgc
     7081 cggtagtcct cgagcacgtt gcgccggtag cgcagccagt ggccgaggcg cgcggcgccg
     7141 ctttccacca cgatcatgcg gatgcgctcg atctgcgggc tctgcagcgc ggtctccacc
     7201 ggaatcctgt tcgaccagac gtagatcagc atggcgtagg gcagcggctc tccggcaaac
     7261 acccgcgcca ggcgcagcag ggcgcgctgc tggaagtcga gccgttccgg gtcgccgtga
     7321 aacgacacca cgaggcgcgc caccgagtcc tcgcgcgacg cgacgcgctt gtcggtgccc
     7381 ggcatcaggt catcgacgcg ccagctccat tcgagcagcc cctgacggaa cgggtcgacg
     7441 cgcacgcgcc ggtacaaccc ggtggcagcc cgctcggcgg tagcctgcag cacggtcccg
     7501 gcgctgccgt tcacgaggcg gtaatccgtg tggcgcttgc ccgaatgcag cccccagccc
     7561 acccagtcgg cgggcagcgg cgcgcccacc cgcagtcgcg agaagcgcgc cacttcgacg
     7621 acgtcgcccg catgccgctc gagctcgcgc acgatcggcg tcgaggcgag cagcccgtcg
     7681 tcctcgggca cgacggcgca cccgccgagc gcgagcagcg ccgccagcac ggccgcgggg
     7741 cgcatcagat cgcctcccgc ggcgcgcgcc gcagcggccc gtccaccgcc gaggcgaggc
     7801 cgccgagcgc ctccagcagc gcgtcgcggt tcagccggta cagcacgcgc tggccctggc
     7861 gctcggccga gaccagcccg gcgcgctgca gcaccgccag gtgccgcgac accgcgggcg
     7921 ggctcatgcc gaagcgctcc gcgagatcgg tggtcgagag cgccgtctgc gcgaggaagg
     7981 cgagcgcccg gcgccgcggc aacgaggcca gcgcggcgaa cacctcgtcc tgaggattag
     8041 catccatgtt aatagtatgg cataggcggc gccgcgctgg ttagaatggc gcctcactca
     8101 aggagacccc gttccatggc gaaagcaaag gcggcgccga agggcccgct ctcgatggcc
     8161 gaccgcgacg gctggatctg gcacgacggc aagctggttc cctggcgctc ggccaccacc
     8221 cacgtgctca cgcattccct gcactatgga ctcgcggtgt tcgaaggcgt tcgcgcgtac
     8281 aagaccgtgc gcggcacggc gatctaccgg ctgaaggagc atacgcagcg gctcatcaac
     8341 tcggcgcaca tctacatgat gaagatcccg tacacgctcg agcagctgat gaaggcgcag
     8401 atcgaggtgg tgcgcgccaa cggccacgag gcctgctacg tgcgcccgat cgccttctac
     8461 ggctcggaga agatgggcgt gtcgcccgtc ggcgcgcgcg tgcacgtggc gatcgccgcc
     8521 tggccctggg gcgcctacct cgggcccgaa gcgctggaaa aaggcatccg cgtgaagacc
     8581 tcctcgtacg cgcgccacca catcaacgtc accatggcgc gctccaagat gtcgggcacc
     8641 taccccaact cggtgctggc gacgctggag gcgacgatgc acggctacga cgagggactg
     8701 ctgctcgacg tcgacggctt cgtcgccgaa ggcgcgggcg agaacatctt catcgtcaag
     8761 gacggcgtga tctacgagcc cgagctcacc tcggcgctca ccggcattac gcgcgcctcg
     8821 gtcatcgagc tggccgccgg cctcggctac aaggtggagt cgcgacgcct gacgcgcgac
     8881 gacatctaca tcgccgacga atgcttcttc accggcaccg cggcggacgt gacgccgatc
     8941 cgcgagctcg acagccgcgt gatcggcgcc ggcaaggccg gcccgatcac caagaagctg
     9001 cagaaggcgt tcttcgacgt ggtcaccggc aaggacaaga aacacacgca ctggctgacg
     9061 ccggtgccga tgcggaaggg caagccgtga gcaccgatac cgcacggcag gtggacgtaa
     9121 ccgagaagga tctgcccctg cactgcccgc tgccgggtgc gccgctctgg gcgcggcatc
     9181 cgcgcgtctt cctggacgtc gccaaggcgg gccaggcgct ctgcccgtac tgcggaacgc
     9241 gctacgtcta caagggcgcg gcacccaggg ggcactgagg cttggcgcgg ctcctcgtcg
     9301 tcgcgcccaa ctggatcggt gatgcgctga tggcgcagcc gctgctctcg cggctgcgcg
     9361 agaatcagcc ccaggcgcgc atcgacgtcg tggcgccggc gtgggtcgcg cccgtgttcc
     9421 ggcgcatgcc cgaagtggcg ggggtcatcg agacgccgtt cgcgcacggc gcgctgcacc
     9481 tgcgcgagcg ctggcgcctg ggccgcaccc tgaaggcgct cggctacgac gaagcgtacg
     9541 ttctgcccaa cgcctggaag gcggcgctgg tgccgttttt cgccggcatc gccaggcgct
     9601 gcggctaccg cggcgagtcg cgctacggcc tgctcaacgt cgtgcaccga aagcagccgg
     9661 ggcgcgagcc gatggccctg cattacgcgc gcctggccgg ggcgcccggc gcgccggtcg
     9721 cccagccgct gccggacccg aagctcgccg tggacgcgcc cgcggtgcgc gccgcgcgcg
     9781 agcgcttcgg cctgccggag cgctatgcag tcctctgccc cggcgcggag tacggcccgg
     9841 ccaagcgctg gccgtacttt cccgagctcg cggcgcggat cgataccccc gtcgtcgtcc
     9901 tgggctcggc gaaggagcgc gagctgtgtg cgacagtcgg cggccgcaac ctcgcgggcg
     9961 agaccacgct cgacgaggcg ctcgacgtgc ttgccggcgc ggcgcgggtg gtgagcaacg
    10021 actcgggcct gatgcacgtg gccgccgcgc tcgggcgccc gttggtggcc gtcttcggct
    10081 cgtccagccc gcggcacacg ccgccgctct cgccgcgcgc gcgcgtcctc tggctcggca
    10141 tcgagtgcag cccgtgctac gcgcgcgact gccccctcgg gcatttccgg tgcatgcgcg
    10201 agatgacggt cgagcgcgtg atgcaggaaa tgcagcagct gccctagcat atgccgcttc
    10261 gcatcttccc cacaccgcag gacgcagagt ccgccttcta cgaggcgctc gaacgcgccg
    10321 acctggagct gatgatggcg gtgtggtcgg aagacgagga gatcgtctgc gtgcacccgg
    10381 gcgggccgcg cctcgccggg caggaccagg tgcgcgacag ctggcgccag atcttcgccg
    10441 gcggcgcaag cgcccgcgtg cacgttacgc agcaggtgac ggtcaccggc acgatggtcg
    10501 cggtgcacag cgtgcacgag aacttctcgc gccccgggca ggcgcgcccg gtgcaggcgg
    10561 tggtggcgac caatgtctac ctgcgtacgg cggcgggctg gcgcatggtg gcgcaccatg
    10621 gctcgcccgc gccgggcagc gccgagccgc cgccgcccgc gccgaagatc ctgcattgag
    10681 cgcataccac gcaccctggt ggctgccggg cggccacctg cagaccgtgt acagcgcgct
    10741 cgggccggcg ccgcgcatcg cctggcgccg cgaacgctgg gacacgccgg acggcgactt
    10801 catcgacctc gactgggccg agggcggccg cggcccgctg ctcgcgctct ttcacggcct
    10861 cgagggcggg tcctcgagcc actacgcgcg caaagtcgcc gcgcaggcgc agcggcgcgg
    10921 ctggcgctgc gtggtgccgc acttccgcgg ctgctcgggc gaggccaacc ggcacgcgcg
    10981 cgcctaccat tccggcgaca gcgcggagct cgactggatc ctgcggcgcc tgcggcccga
    11041 tttcgcggcg ggcgtgtcgc tcggcggcaa cgtgctgcgc aagtggctcg gcgagcaggg
    11101 cgcggccgcc gcgcagctcg tgcgacgcgc tgccgccgta tcggcgccgg tcgacctcgc
    11161 ggcgtcgggg cacgccctcg accgcacgct caaccgctgg ctgtacgcgg cgcactttct
    11221 caccacgctg aagcccaaag cgctcgccaa gatctcgcag catgcgctcg cgatcgatgc
    11281 gacgcgcgtc tcgcgcgcgc gcacgctgca tgagttcgat gacctggtca ccgccccgct
    11341 gcatggcttt cgtgacgccg acgactactg gacgcgcgcc gccagcggcc cgtggctcga
    11401 gcgcattcgc gtgccgacgc tggtgctcaa cgccatgaac gatccttttc tgccgcacgc
    11461 cgcgctcgcc gcagcagcgc gccgggcgtc gcctgacgtg gtgctagaat ttcccgcaac
    11521 cggcggacat gccggctttc cgggacgcgg acactggctt gcgcgccggg tcctggagtt
    11581 ctgcgccgca ccgtgaccat tgccgccgaa atctttcgcg cctacgacat ccgcggcatc
    11641 gcgggcaaga cgctgactgt ggacgccgtg cgcggcatcg gccgcgcgct cggctcgatg
    11701 gcggacgagt tcgccgtggg tcgcgacggc cgcgactccg ggcccgcgct ggtcaatgcg
    11761 ctgtgcgaag gcttgcagcg ctccggcgcc agcgtgatcg acatcggcat ggcgccgacg
    11821 ccggtcacgt acttcgcggc gcaccagctg aactgtggca gctgcgtctc ggtgaccggc
    11881 agtcacaacc cgcccgagta caacggcctg aagatggtgg tcgacggcca cacgctctcg
    11941 ggcgacgaaa tccagggact gcgccggcgc atcgagcgcg gcgagttccg cgccggcgcc
    12001 ggccgccggc gggaggccga cgtgctcgac gcctacgttg agcgcatcac cggcgacgtc
    12061 aggctcgcgc ggcgcatgcg catcgccacc gattgcggca acggcgtcgc cggcatggtg
    12121 gcgccgcgcc tgtaccgcgc gctcggctgc gaggttcggg atctgtacag cgacgtggac
    12181 ccgaccttcc ccaaccatca cccggatccg tcccataccg agaacctggc cgacctgatc
    12241 gccgccgtgc gcgacggcgg ggccgagctc ggccttgcct tcgacggcga cggcgaccgg
    12301 ctcggcgtcg tgacccggga cggcagcgtc atctacccgg accggcagct gatgctcttc
    12361 gccgccgacg tgctgtcgcg caatccgggc gccgagatca tctacgacgt gaagtccacc
    12421 cgcctccttg cgccgtggat cgagcgccac ggcggcaagc cgctgctgtg gaagaccggg
    12481 cactcgctga tcaaggcgaa gctcgccgag accggcgcgc cgctcgccgg cgagatgtcg
    12541 ggccatacgt tcttcaagga gcgctggtac ggcttcgacg atgcgctcta cgcgggcgcg
    12601 cgcctgctcg agatcctgtc gcgctcgcac gacgcgagcg ccgtgctcac ctcgctgccg
    12661 gacgcgccct cgacgccgga gttgcagtgg aagctcgccg agggcgagcc gcatgcgctg
    12721 atcgcgaagc tgcaggcagc gaagcccttt cccaaggccg cgcgcaccat cactatcgac
    12781 ggcgtgcgcg ctgagtacgc cgacggcttc ggcctggcgc gcgcctccaa taccaccccg
    12841 gtgatcgtgc tgcgcttcga ggcggatacg aaggcggcgc tggcgcgcat ccaggacgag
    12901 ttccgcgccg cgctgcagcc gctcaagccc ggcgcgccgc tgccctacta ggcgaagtcc
    12961 gggccgatcc ggtcgagccg gtcgctgacc aggcaatcca cgttccagcc aaggagcttg
    13021 cgcgccgcgc gccggtcgtt gacggtccag gcgagcacgg cgcagccggc ggcatgggct
    13081 tcctgcacca cgcgctgccg cagcgagcgg aagttggcat gcagcgcgac gcactccagg
    13141 tcgcgcagca tctcgcgcca gtgcggcagc aggcgcccaa gcagcaggcc gcgcggcagc
    13201 tccggcgcgg cttgccgggc ggcggcgagc gcaaccggcg aaaacgagga cagaacgggc
    13261 ggcagcgccg cgccccgcca gagctcggcg gccatgcgcg ccaccgtttc gcccgtgcgc
    13321 cgctcgtagc cgcgccccgg cttgatctcg acgttggccc acagcccgag gtggcggcac
    13381 aggcgcaccg cgtcctcgaa gcgggggatc ttctcgccgc gccaccgcgc gccgcgccac
    13441 gcaccggcgt cgaggcgttg cagttccgcg tacggcgttt gcgcgacgta accgtgcgcg
    13501 ccggtggtgc gcctcagggt ctcatcgtgg atcagaaccg gcgtgccgtc gccggcgagc
    13561 atgacgtcga actcgacgcc cttgaagccc atctcggcgc cgagccggat cgcgccgagc
    13621 gtgttctcgg gcgcgagcgt gccgccgccg cggtgcgcca caatgcgcgg gtagggccag
    13681 tggctcatgc gggccggtgc tccggcttga ggcgcacgta gagagtccgg tgcacgcggg
    13741 ccacgaccgc gcgctgcgca tcgacgacgt cggtgacgaa ctcgggaagg accttctctc
    13801 cgcccgcggc ctgcgcgcgg atcttcgcga ggtgctcctc ggtgagcgaa aagcgcgccg
    13861 tgacggtgcc gcgcgcgggc gcgaggtact cgatcgaggc cgatttgtcc cagacgtagt
    13921 actgcgggcc gagattttgc atcagcatga gcatgtagaa ggcgtcggtc atcgcgaaca
    13981 ggcttccgcc gaattgagcg ccggcgatgt tgcggttgag ccagcccagg cgcagctccg
    14041 cctcggcctc gcggaagtcg tccgtcatgc gccgcaggcg gatgccgggg ctgcggaatg
    14101 ggccccacca gtgcatgccc cgcaccagcg tacgcgcgct gaacttcacg cgctcagcct
    14161 tcgatccgct tgccgctctg cgggtcgaac cagtgcacct gcgccgggtc gtagcgcagc
    14221 ggcagcttcc cggcgcgcgc gtcgaccgag gcgtgcagcc gcgcggccac ccgcgcgccc
    14281 tcgccgtcgc cgaagtggcc gtagacgagc gtatcggcgc cgagcggctc gatcaggtcc
    14341 acttccagcg tgatcg
//
LOCUS       DBNHKE010000122        11202 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1342738, whole genome shotgun sequence.
ACCESSION   DBNHKE010000122 DBNHKE010000000
VERSION     DBNHKE010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1342738"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="AC16O9_07690"
     CDS             complement(<1..106)
                     /locus_tag="AC16O9_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR6516536.1"
                     /translation="MIDRRRFLQALAAAGLSRALPGCAQSAPRFAGYPF"
     gene            144..425
                     /locus_tag="AC16O9_07695"
     CDS             144..425
                     /locus_tag="AC16O9_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR6516537.1"
                     /translation="MIHFVVHEEGDGVGVVVVEGVKQGQELTGWIMEDDKEIRITAKS
                     DIPIGHKLALKDYKPGDTVIKYGVDIGKVVAPIAKGEHTHIQNLKTKRW"
     gene            516..1208
                     /locus_tag="AC16O9_07700"
     CDS             516..1208
                     /locus_tag="AC16O9_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6516538.1"
                     /translation="MHGAWHGGWCWRRVAERLEKAGHRVFTPTLTGLGERAHLLSSGV
                     NLETHVHDVVGVLEAEELENVVLAGHSYAGLVISGVAARAKKRLRQLVYLDALLVEDG
                     KSWAEAFPPEVTEARRKSATMTNGVKTLPAPDPAIYGFADPADDEWLRRRLTPHPYAP
                     FEQQIRCGCPLGNGLPKAYVDCTEPALAALSAMKARWRSGADWEVIELKTGHDLMVSA
                     PADTAQILLRYA"
     gene            1205..1921
                     /locus_tag="AC16O9_07705"
     CDS             1205..1921
                     /locus_tag="AC16O9_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR6516539.1"
                     /translation="MSAVLKVRVWRGGADGAFREYEVPRRESQTVLDVVTHIQRTLDP
                     TLAYRYACRVGMCGSCAMTVNGAPRWTCRTHVDKVAGDGRLTVGPLRNLPVIRDLVAD
                     MREFFDKWAHARGQFRGARTRADDVERVSPQSAARRRADAAIECIGCGVCYAACDVVA
                     ANPDYLGPAALNRAWTLVNDARDTGNDERLRAVAGDGASLACHTHMSCTERCPKALAP
                     TAGIAGLKRAVARAALRGKL"
     gene            1972..2256
                     /locus_tag="AC16O9_07710"
     CDS             1972..2256
                     /locus_tag="AC16O9_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR6516540.1"
                     /translation="MLGVCVLVHLATIIYAVQGGLTGAEILARTRGNVAWAAFYTVFV
                     VAVAVHAPIGLRSVLAEWLGWRGRSRDFLLIAFAVVLAGMGLQAVRGVFA"
     gene            2253..2555
                     /locus_tag="AC16O9_07715"
     CDS             2253..2555
                     /locus_tag="AC16O9_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR6516541.1"
                     /translation="MSRALALAHRLSGVALALFLPAHFWVLARALQRDAFFAWTEQPA
                     VKLAEWGLVTLLALHLAGGLRVLALEFLPWRDWAKALAALAAGFAIFAGLAFALAL"
     gene            2590..3555
                     /locus_tag="AC16O9_07720"
     CDS             2590..3555
                     /locus_tag="AC16O9_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6516542.1"
                     /translation="MKIVKSLTACAAGLLLSLSAVAQTWPHKPVRFIVPFPPGGATDI
                     SARLLSEKLTQMWGQSVVIENRGGAGGGVGAAEAAQMTPDGHNFFFPSGSVVTANQHI
                     YAKLNYDPEKDFVPVTNVVTGPQVLVVPASSPFKSLQELIAAVKKEPGKYTFGHAGIG
                     SQIHLAAENFVYQAKLDAISVPYRGEGPALAGLVGGETTFMLPNMAAAIGHVNAGRVR
                     ALGVTSAEEAPQMPGVPPIGRTVKGFVNSGWFGIVAPAGTSPQTIDRMYNDVRKALED
                     TRMRARFYAMGLTPVGNSPAEMAKALKEETKLWAVVVRERKISVK"
     gene            3552..4427
                     /locus_tag="AC16O9_07725"
     CDS             3552..4427
                     /locus_tag="AC16O9_07725"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR6516543.1"
                     /translation="MKVDLKSVEEAAKELYIRALKALPPDIKQGLDALAKRERDAGAK
                     RMLGTMIRNISVAEDTDNLLCQDTGIPVYNVRIGRGVQVEGAALKEAIRAGCARSTRE
                     YPFRSSIVHPTTRFNEQTSCGRGIPVINFDFTDAPDVLEIEMIPKGSGSENNSWLKMA
                     IPAEGVDAIKTFVVDCVLQAGGNTCPPTIVGVGVGGTADLCVRLAKVAATRPLGSECE
                     DAEGAKLEHALSEAVNLLGVGPQGLGGDSTAFAVHVETAATHITMNPVAVNMQCHSAR
                     RARATIRPRGIEYGY"
     gene            4417..4968
                     /locus_tag="AC16O9_07730"
     CDS             4417..4968
                     /locus_tag="AC16O9_07730"
                     /inference="COORDINATES: protein motif:HMM:NF027363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HIR6516544.1"
                     /translation="MVTRALLLLAFALPLQAAEVAGVKFDDQARIADTQLALTGAGLR
                     QRFIFDVYAMGLYVRDAKADPITQPGAKRIVIRMLRNVDADTFTQALVDGMRPNHDAA
                     TMQALAPRIGELTAIMAQLKEAKKGMTITLDWLPATGTQMSVNGQFTGAPIAGEDFYQ
                     ALLRIWLGPQPVQEDLKKALLGG"
     gene            4974..5582
                     /locus_tag="AC16O9_07735"
     CDS             4974..5582
                     /locus_tag="AC16O9_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6516545.1"
                     /translation="MAHHDFNMPVSEAQVRSLRVDDTVTLNGTLYGIRDATQIHMFDR
                     GRKTRFDLKGHAVIHTAPNVKKVEKSAAHPAGYAPICVGTTTGERMERFSRPLMREYG
                     VRLIIGKGGLREDSLAAFGELGGAYLAIVGGAAALETTWVEAIEDVDLDDLYPESLWK
                     FRVRGFGPLLVAMDSHGGSLYARVRDASQAKRADALRRLGVG"
     gene            5622..6578
                     /locus_tag="AC16O9_07740"
     CDS             5622..6578
                     /locus_tag="AC16O9_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6516546.1"
                     /translation="MKWLLRVAALAAGSLAFAVQAQNLSIGTGGTGGVYYPLGGGIAA
                     VLSKYVSGMQATAEVTGGSVANLQLVGTGKPYLAMTMVDAGLDAYKGQQKFEGKAVPV
                     RTLMVMYPNRMHVVSITATGIKSIKDLKGKRVSTGSGGSATEVMAFRVIEAAGLDKDK
                     DMTRERLGVAESVNALKDRKIDAFFWVGGLPTAAVTDLANSPGVTIQMIDHADLVPAM
                     NQKWGNLYVKDVISKDVYKGMAADNQQATVMNLMVAHEKMDEQTAYNVVKAIFEHREE
                     LIRVHKEAENFTLDKQKTAATGGIPWHPGALKYFKEKGVKLD"
     gene            6647..8677
                     /locus_tag="AC16O9_07745"
     CDS             6647..8677
                     /locus_tag="AC16O9_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR6516547.1"
                     /translation="MSEDIARLHQPAKEVSEEALARAEEMIEREEGVHNKLRGWIAAL
                     VATVAVAMSLFHLYTAYAIVRPEHLRAIHVAFVLFLTFLIFPVAKRFRHRVMWWDWLF
                     AIAGVATAVYLIRGGDDFFDRSIVPNDWDIVFGVGLALLILEAMRRTTGWIMPFVTIC
                     FLAYAFFGEYLPAPWTHKGYEIPRLVGHMYMTLEGVYGTAIAVSSTLIILFTIYGAFL
                     QFSGAGKFFIDFSFAAMGGKPTGAGRTVTLASFLLGGPSGSGVATTVTLGAVAAPMLA
                     KVGYGKNEAGGLLAAGGLGAIISPPVLGAAAFLIAEFLKISYLDVLLMAVIPTCLFYL
                     TLFIMVEIDSRKFGMKDAVFEKVETAWSLTKKYWFHFFSLVSIVGFMLMGFSPELSVF
                     WATVVVFATSFLHRDCALFSYDWLRGREPFVRGFLGSGLIRALDNGSRAVLNVAATCA
                     GAGLIVGVVTLTGLGLKFSSIVLAYAGNSLLLTAIYTALIVWIVGLAVPVTASYIICA
                     VIAAPALIALKVPEFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQTWK
                     YTTPAFLVPFIFVLDPSGTGLLLTGSFKTLASANWASIAYITFTAAVGIMSLAGAFQG
                     WLFKRTPLYERLMLLVAGFALVYPGNLSNGIGFVLVVLALASQWARRERARA"
     gene            8664..10376
                     /locus_tag="AC16O9_07750"
     CDS             8664..10376
                     /locus_tag="AC16O9_07750"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR6516548.1"
                     /translation="MRALDTDILILGAGGAGLFAALHAHRADPSLRITVAVKGLLGKC
                     GCTRMVQGGYNVALAKEDSVERHFMDTIEGGKWLPDQELAWKLVTLAVERIRELENEL
                     GCFFDRNADGTIHQKAFAGQTFDRTVHKGDLTGIEIISRLAEQVWARNIQRLEEHRAV
                     ELVRTPAGDALAGVLMIDMRTGEYVMVRAKAVLLATGGGPTMYRYHTPSGDKSCDGLA
                     MALRAGLALRDMEMVQFHPTGLLAGPHTRMTGTVLEEGLRGAGGYLLNGARKRFMPDY
                     DPLAERATRDFVSRAMYTEMRKGEVTPNGGLYISMAHLGPDKVARDFKGMVERCADCG
                     FDLAGGLVEVVPTAHYMMGGVEFEPDCSTALPGLFVAGEDAGGVHGANRLGGNGVANS
                     TVFGAVAGDTMAAWVAREGVLRAPDQAALAAAVERCEPKTAGAHGALEPLRERLHEVM
                     WEKVGIIRDATRLRAALSELDAIAAELAASGIPDTDRAYNLTWHDWLNLRSLTEVSRV
                     IAQAALAREDSRGAHFREDCPATSALERSAYSSVRAGAGVSMKPVAFTRVKPGESLLT
                     RGAY"
     gene            complement(10357..11028)
                     /locus_tag="AC16O9_07755"
     CDS             complement(10357..11028)
                     /locus_tag="AC16O9_07755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6516549.1"
                     /translation="MGSMIELKAADGHKLAAYRAEPSGKPRGAVVVIQEIFGVNKHIK
                     AVADGYAADGYLVIAPSMFDRMQRGYDTGYSPPEIEAGRALMQKADWKQTMQDAAAAL
                     EAAKAGGKVGIVGYCWGGTVTWVAAARLSGLACAVPYYGGGMPGFIGEKPKCPTMCHF
                     GEQDQTPSLEQAKQIAKAHPEITAHYYPGAGHGFNCDHRPSFNAEASKLARSRTLEFF
                     SKHLG"
BASE COUNT         1763 a         3766 c         3931 g         1742 t
ORIGIN      
        1 cgaacgggta gcccgcgaag cgcggcgcgc tttgcgcgca gccgggcagg gcgcgcgaaa
       61 ggcccgcggc ggccagagcc tgcaggaacc tgcgccgatc aatcatgccg atagaatagc
      121 gggggaacgt tacggagtcg gcaatgattc attttgtcgt gcacgaggag ggcgatggcg
      181 tcggggtggt cgtcgtcgaa ggcgtgaagc agggccagga gctcaccggc tggatcatgg
      241 aggacgacaa ggagatccgg atcacagcga agagcgacat cccgatcggc cacaagctcg
      301 ccctgaagga ctacaagccc ggcgacacgg tgatcaagta cggcgtggac atcggcaagg
      361 tcgtcgcgcc gatcgccaag ggcgagcaca cgcacatcca gaacctgaag accaagcggt
      421 ggtgaccatg cgcccgtcca acgcggccga ggcgctcggg catcgcgagc tcgtgccgaa
      481 gaggctgtac gagtccgcct agcaccttcg ttctcgtgca cggcgcctgg cacggcggct
      541 ggtgctggcg ccgtgtcgcc gagcgcctgg aaaaggcagg acaccgcgtg ttcacgccca
      601 cgctcacggg cctcggcgag cgcgcccact tgctgagctc cggggtgaac ctcgagacgc
      661 acgtgcacga cgtcgtcggc gtgctcgagg ccgaagagct ggagaacgtc gtcctcgccg
      721 gccacagcta cgccggcctc gtgatctccg gcgtggcggc gcgcgcgaag aagcgcctgc
      781 ggcagctcgt ctatctcgac gcgctgctcg tggaggacgg gaagtcctgg gccgaggcct
      841 ttccgcccga ggtgaccgag gcgcggcgca agtcggcgac gatgaccaac ggcgtcaaga
      901 cgctgccggc gccggatccc gcgatctacg gcttcgccga tccggccgac gacgaatggc
      961 tgcgccggcg actgaccccg cacccctacg cgcctttcga gcagcagatc cgctgcggct
     1021 gccccctggg caacggcctg cccaaggcct acgtcgattg caccgagccg gcgctcgccg
     1081 cgctgagcgc gatgaaggcg cgctggcgca gcggcgcaga ctgggaggtg atcgagctga
     1141 agaccggcca cgacctcatg gtgagcgcgc ccgcagacac cgcgcagatc cttctgcgct
     1201 acgcatgagc gcggtcctca aggtgcgcgt ctggcgcggc ggcgccgacg gcgcctttcg
     1261 cgagtacgag gtgccccgcc gcgaaagtca gaccgtgctc gatgtggtca cgcacatcca
     1321 gcgcacgctg gatccgacgc tcgcctaccg ctacgcctgc cgcgtcggca tgtgcggctc
     1381 ctgcgccatg acggtgaacg gcgcgccgcg ctggacctgc cgcacgcacg tcgacaaggt
     1441 ggccggggac ggccgcctca ccgtcggccc gctgcgcaac ctgccggtga tccgcgatct
     1501 cgtggccgac atgcgcgaat tcttcgacaa gtgggcgcac gcgcgaggcc agttcagggg
     1561 cgcgcgcacg cgcgccgacg atgtcgagcg ggtgtccccg cagtcggcag cgcgccggcg
     1621 cgccgatgcc gccatcgagt gcatcggctg cggcgtgtgc tacgcggcct gcgacgtggt
     1681 ggcggccaat ccggactacc ttggcccggc agcgctcaac cgcgcctgga cgctcgtcaa
     1741 tgacgcgcgc gacacgggga acgacgagcg cctgcgcgcg gtcgcgggcg atggcgcaag
     1801 cctcgcctgc cacacgcata tgagctgcac cgagcgctgc ccgaaggcgc tcgcgcccac
     1861 cgccggcatc gcgggcctga agcgcgcggt ggcgcgcgcg gcgctgcggg ggaagctctg
     1921 agcgcgcgcg gccagacgct cctctggatc gcgcagcgcg cgagcgccgc ggtgctcggc
     1981 gtgtgcgttc tggtgcacct tgccaccatc atctacgcgg tacagggcgg gctgacgggc
     2041 gccgagatcc tcgcgcgcac gcgcggcaac gtcgcctggg cggcgttcta cacggtattc
     2101 gtcgtcgccg tcgcggtgca cgcccccatc gggctgcgca gcgtgctcgc cgagtggctc
     2161 ggctggcgcg gacgatcgcg cgacttcctg ctcatcgcct tcgccgtcgt gctcgccggc
     2221 atggggctgc aggcggtgcg cggggtgttc gcgtgagccg ggcgctggcg ctcgcgcacc
     2281 ggctgtccgg agtcgcgctc gccctattcc tgccggccca cttctgggtg ctagcgcgcg
     2341 ccctgcagcg cgatgccttc ttcgcctgga ccgagcagcc ggcggtgaag ctggccgagt
     2401 ggggcttggt gacgctgctt gcgctgcacc tcgcgggcgg gctgcgggtg ctggcgctcg
     2461 agttcctgcc gtggcgcgac tgggccaagg cgctggcggc gctcgcggcg ggattcgcga
     2521 tcttcgcggg tctggcgttc gcgctcgccc tgtagcatga tccgcgcagt ttcctaggga
     2581 ggtccagcaa tgaagatcgt gaaatcgctg actgcatgcg cggccggcct gctgctgtcc
     2641 ctgtccgccg tggcgcagac ctggccgcac aagccggtgc gcttcatcgt gccgttcccg
     2701 ccgggcggcg cgaccgatat ctcggcgcgg ctgctctcgg agaagctgac ccaaatgtgg
     2761 gggcagagcg tggtgatcga gaaccgcggc ggcgcgggcg gcggcgtggg cgcggcggaa
     2821 gccgcccaga tgacgccgga cggccacaat ttcttcttcc cttcggggtc ggtcgtaacg
     2881 gcgaatcagc acatctacgc caagctgaac tacgacccgg agaaagactt cgtgccggtg
     2941 accaacgtcg tcaccgggcc gcaggtcctg gtggtgccgg cgtcctcgcc gttcaagtcg
     3001 ctgcaggaac tcatcgccgc ggtgaaaaag gagccgggca agtacacctt cggccacgcg
     3061 gggatcggct cgcagatcca cctcgcggcg gagaacttcg tctaccaggc gaagctcgac
     3121 gcgatctcgg tgccctacag gggcgagggc cccgcgctcg cagggctggt gggcggcgag
     3181 accaccttca tgctgcccaa catggccgcg gccatcggcc acgtgaacgc ggggcgggtg
     3241 cgcgcgctcg gcgtgaccag cgccgaggaa gcgccgcaga tgcccggcgt gccgccgatc
     3301 gggcgcacgg tcaagggttt cgtcaactcc ggctggttcg gcatcgtcgc gccggcgggc
     3361 acttcgccgc agaccattga tcggatgtac aacgacgtgc gcaaggcgct cgaagacacg
     3421 cgcatgcggg cacgcttcta cgccatgggt ctcacgcccg tgggcaacag tcccgccgag
     3481 atggccaagg cgctgaagga ggagaccaag ctctgggcgg tggtcgtgcg ggagcgtaag
     3541 atctccgtga agtgaaagtc gacctgaaaa gcgtcgagga ggccgcgaag gagctctaca
     3601 tccgggcgct gaaggcgctg ccgcccgaca tcaagcaggg gctcgacgcg ctcgccaagc
     3661 gcgagcgcga cgcgggcgcg aagcgcatgc tcggcacgat gatcaggaac atcagcgtcg
     3721 ccgaggacac cgacaacctg ctgtgccagg acaccggcat cccggtgtac aacgtgcgca
     3781 tcgggcgcgg cgtgcaggtc gagggcgccg cgctgaagga agccatccgc gcgggctgcg
     3841 cgcgctccac gcgcgaatac ccgtttcgct cctcgatcgt gcatcccacc acccgcttca
     3901 acgagcaaac ctcctgcggc cgcggcatcc cggtgatcaa cttcgatttc accgacgcgc
     3961 ccgacgtcct cgagatcgag atgatcccga agggctcggg ctcggagaac aattcctggc
     4021 tgaagatggc gatcccggcc gagggcgtcg acgccatcaa gaccttcgtc gtcgactgcg
     4081 tgctgcaggc gggcggcaac acctgcccgc cgaccatcgt cggcgtgggc gtgggcggca
     4141 ccgccgacct gtgcgtgcgc ctggcgaagg tcgcggcgac gcgcccgctc ggcagcgagt
     4201 gcgaggacgc cgagggcgcg aagctcgagc acgcgctcag cgaggcggtg aatctgctgg
     4261 gcgtcgggcc gcagggtctg ggcggcgatt cgaccgcctt cgccgtgcac gtggagaccg
     4321 cggccacgca catcaccatg aacccggtgg cggtgaacat gcagtgccat tcggcgcggc
     4381 gcgcgcgcgc caccatccgg ccccggggga tcgaatatgg ttactagagc tttgctgctg
     4441 cttgcgttcg cgctgccgct gcaggcggcc gaggtcgccg gcgtcaagtt cgacgaccag
     4501 gcgcgcatcg ccgatacaca gcttgcgctc accggcgcag ggctgcgcca gcgcttcatc
     4561 tttgacgtgt acgccatggg cctatacgtg cgcgacgcga aggccgaccc gatcacccag
     4621 cctggcgcga agcgcatcgt catccgcatg ctgcgcaacg tcgacgccga caccttcacc
     4681 caggcgctcg tggacgggat gcgcccgaac cacgacgcgg cgaccatgca ggcgctcgcg
     4741 ccgcgcatcg gcgagctgac ggcgatcatg gcgcagctga aggaagcgaa gaagggcatg
     4801 acgatcacgc tcgactggct gcccgcgacc ggcacgcaga tgagcgtcaa cggccagttc
     4861 accggcgcgc cgatcgccgg ggaggacttc taccaggcgc tgctccggat ctggctcggc
     4921 ccgcagccgg tgcaggaaga cttgaagaag gcgctgctcg gcggctagcc cggatggcgc
     4981 accacgactt caacatgccg gtaagcgagg cgcaggtgcg cagcctgcgc gtcgacgaca
     5041 cggtcaccct caacggcacg ctctacggca tccgcgacgc cacgcaaatc cacatgttcg
     5101 accggggcag aaagacgcgc ttcgacctga aggggcatgc ggtgatccat accgcgccca
     5161 acgtgaagaa ggtcgagaag agcgccgccc acccggccgg ctacgcgccg atctgcgtcg
     5221 gcaccacgac cggcgagcgc atggagcgct tcagccgccc gctgatgcgc gagtacggcg
     5281 tgcgcctgat catcggcaag ggcgggctgc gtgaggactc gctcgcggca ttcggcgagc
     5341 tgggcggggc ctatctggcg atcgtcggcg gcgccgccgc gctcgagacc acctgggtgg
     5401 aggccatcga ggacgtggac ctcgacgacc tgtacccgga gtcgctgtgg aagttccgcg
     5461 tccgggggtt cgggccgctg ctcgtcgcca tggacagcca cggcggcagc ctgtacgcca
     5521 gggtgcgaga cgcctcgcaa gcaaagcgcg ccgacgcgct gcggcggctc ggcgtggggt
     5581 agaatccgcg ccgcagttcc ctaccctagg aggaagtcat catgaaatgg ctgttgcgcg
     5641 tcgcggcgct tgccgccggc tcgctcgcgt tcgccgttca ggcgcaaaat ctttccatcg
     5701 gcaccggcgg cacgggcggc gtgtactacc cgctcggcgg cggcatcgcc gccgtgctct
     5761 cgaagtacgt ctccgggatg caggctaccg ccgaagtcac cggcggctcg gtggccaacc
     5821 tgcagctcgt cggcaccggc aagccctacc tcgccatgac catggtggac gcggggctcg
     5881 acgcctacaa gggtcagcag aagttcgaag gcaaggccgt gcccgtgcgc acgctgatgg
     5941 tgatgtaccc gaaccgcatg cacgtggtat cgatcacggc gacgggcatc aagtcgatca
     6001 aggacctgaa gggcaagcgc gtctccactg gctcgggcgg cagcgccacc gaggtcatgg
     6061 cgttccgcgt catcgaggcc gcgggcctgg acaaggacaa ggacatgacg cgcgagcgcc
     6121 tcggtgtggc cgagtcggtg aacgcgctga aggaccgcaa gatcgacgcc ttcttctggg
     6181 tcggaggcct gccgacggcg gcggtgaccg acctggcgaa ctcgcccggc gtgacgatcc
     6241 agatgatcga ccacgcggac ctggtgcccg ccatgaacca gaagtggggc aacctgtacg
     6301 tcaaggacgt gatctcgaag gacgtctaca aggggatggc ggccgacaac cagcaagcca
     6361 ccgtcatgaa cctgatggtg gcgcacgaga agatggacga gcagaccgcc tacaacgtcg
     6421 tcaaggcgat cttcgagcac cgcgaggagc tgatccgcgt gcacaaggag gccgagaact
     6481 tcaccctcga caagcagaag acggcggcga ccggcggcat tccctggcat ccgggcgcgc
     6541 tgaagtactt caaggaaaag ggcgtcaagc tggactaagg tccggcgtca cccgggcccc
     6601 gtccaggtaa gctggcgggg ccttttcgtt tctgagaggg gggacgatga gcgaagacat
     6661 cgcccggctg caccagccgg caaaggaagt cagcgaagag gcgctggcgc gcgccgagga
     6721 gatgatcgag cgcgaggaag gcgtccacaa caagctcagg ggctggatcg ccgcgctcgt
     6781 cgccacggtg gcggtggcga tgtcgctgtt ccacctgtac acggcctacg ccatcgtgcg
     6841 ccccgagcac ctgcgcgcga tccacgtcgc gttcgtgctg ttcctcacct tcctcatctt
     6901 cccggtggcc aagcgctttc gccaccgcgt catgtggtgg gactggctgt tcgccattgc
     6961 gggcgtggcc accgccgtgt acctgatccg gggcggcgac gacttcttcg accgctcgat
     7021 cgtcccgaac gactgggaca tcgtgttcgg cgtggggctt gcgctgctga tcctggaggc
     7081 gatgcggcgc acgacgggct ggatcatgcc gttcgtcacg atctgcttcc tcgcctacgc
     7141 gttcttcggc gagtacctgc ccgcgccctg gacgcacaag ggctacgaga tcccgcgcct
     7201 cgtcggccac atgtacatga cgctggaggg cgtctacggc acggcgatcg ccgtgtcctc
     7261 gacgctgatc atcctgttca ccatctacgg cgcgttcctg cagttctcgg gtgcgggcaa
     7321 gtttttcatc gatttttcct tcgccgcgat gggcggcaag ccgacgggcg cgggccgcac
     7381 cgtaacgctc gcctcgttcc tgctcggcgg cccctcgggc tcgggggtcg ccacgacggt
     7441 gaccctgggc gcagtcgcgg cgccgatgct ggcgaaggtg ggctacggca agaacgaggc
     7501 gggcgggctg ctcgccgccg gaggcctggg cgccatcatc tcgccgccgg tgctgggcgc
     7561 ggcggcgttt ctcatcgccg agttcctgaa gatctcgtac ctcgatgtgc tgctgatggc
     7621 ggtgatcccg acgtgcctgt tctacctgac gctgttcatc atggtggaga tcgattcgcg
     7681 caagttcggc atgaaggacg cggtgttcga gaaggtggag acggcctgga gcctgaccaa
     7741 gaagtactgg ttccacttct tctcgctggt ctcgatcgtc ggcttcatgc tgatgggctt
     7801 ctcgcccgag ctgtcggtgt tctgggccac cgtggtggtg ttcgcgacga gcttcctgca
     7861 ccgcgattgc gcgctctttt cctacgactg gctgcgcggg cgcgagccct tcgtgcgcgg
     7921 cttcctcggc tcgggcctga tcagggcgct cgacaacggc tcgcgcgcgg tgctcaacgt
     7981 cgccgccacc tgcgccggcg cgggcctcat cgtcggcgtg gtgacgctca ccggcctggg
     8041 gctgaagttc agctccatcg tcctcgccta cgccgggaac tcgctgctcc tgaccgcgat
     8101 ctacacggcg ctgatcgtct ggatcgtggg cctcgcggtg ccggtgacgg cgagctacat
     8161 catctgcgcg gtaatcgccg cgccggcgct gatcgcgctc aaggtccccg aattcgcggc
     8221 gcacatgttc atcttctact acgcggtgct ctcggaagtc tcgccgccca cggcgctctc
     8281 gccgttcgcg gccgccgcga tcacgggggg cgatccgtac aagacgacgc tgcagacctg
     8341 gaagtacacc acgcccgcgt tcctcgtgcc cttcatcttc gtgctcgatc cgtcgggcac
     8401 ggggctcctt ctcaccggct cgttcaagac gctggcgagc gcgaactggg cgtcgatcgc
     8461 ctacatcacc ttcaccgcgg cggtcggcat catgtcgctc gctggcgcgt tccaggggtg
     8521 gctgttcaag cgcacgccgc tctacgagcg tctgatgctg ctggtagcgg gcttcgcgct
     8581 ggtgtatccc ggcaacctct cgaacggcat cggcttcgtg ctggtggtcc tggcgctggc
     8641 ttcccagtgg gcacgcaggg aacgtgcgcg cgcttgatac cgacatcctg atcctcggcg
     8701 cgggcggcgc gggcctgttc gccgcgctgc acgcgcaccg cgccgatccg tcgctgcgca
     8761 tcacggtggc ggtaaagggg ctgctgggga aatgcggctg cacgcgcatg gtgcagggcg
     8821 ggtacaacgt ggcgctggcg aaggaagact ccgtcgagcg ccacttcatg gatacgatcg
     8881 aaggcggcaa gtggctgccc gaccaggagc tcgcctggaa gctggtcacg ctcgcggtgg
     8941 agcgcatccg cgagctcgag aacgagctcg gctgcttctt cgaccgcaac gccgacggca
     9001 ccatccacca gaaggccttc gcggggcaga cgttcgaccg caccgtgcac aagggcgatc
     9061 tcaccggcat cgagatcatc agccgcctcg ccgagcaggt gtgggcgagg aacatccagc
     9121 ggctggaaga gcatcgcgcc gtggagctcg tcaggacgcc tgcgggcgat gcgctcgccg
     9181 gcgttctcat gatcgacatg cgcaccgggg agtacgtgat ggtgcgcgcc aaagcggtgc
     9241 tgctcgccac cggcggcggg ccgacgatgt accgctatca cacgccctcg ggcgacaaga
     9301 gctgcgacgg gcttgccatg gcgctgcgcg cggggctggc gctgcgcgac atggagatgg
     9361 tgcagttcca ccccacgggg ctgctggcgg gtccgcatac ccggatgacc ggcacggtgc
     9421 tcgaggaagg cctgcgcggc gccggcggct atctgctgaa cggcgccagg aaacgcttca
     9481 tgcccgacta cgacccgctt gccgagcgcg ccacgcgcga cttcgtctca cgcgcgatgt
     9541 acacggaaat gcgcaagggc gaggtcacgc cgaacggcgg cctgtacatc tcgatggcgc
     9601 acctcggccc ggacaaggtg gcgcgcgatt tcaagggcat ggtcgagcgc tgtgccgact
     9661 gcggcttcga cctggccggc ggcctcgtcg aagtcgtgcc gacggcgcac tacatgatgg
     9721 gcggcgtgga gttcgagccc gattgcagca ccgcgctgcc cgggctgttc gtcgccggtg
     9781 aagacgcggg tggcgtgcac ggggcgaacc gcctcggcgg caacggcgtc gccaactcga
     9841 ccgtgttcgg cgcggtggcg ggcgacacga tggctgcctg ggtggcgcgg gagggcgtgc
     9901 tgcgcgcacc cgaccaggcg gcgctcgccg ctgcggtcga gcgctgcgag ccgaagacgg
     9961 cgggggcgca tggcgcgctg gagccgctgc gcgagcggct ccatgaagtg atgtgggaaa
    10021 aggtcggcat catccgcgac gcaacccgat tgcgcgcggc gctttccgag ctcgacgcga
    10081 tcgcggcgga gctcgccgcg agcggcattc ccgacaccga tcgtgcgtac aacctgacct
    10141 ggcacgactg gctgaacctg aggagcctga ccgaggtgag ccgcgtgatc gcgcaagccg
    10201 cgctcgcgcg cgaggattcg cgcggcgcgc acttccggga ggactgtccc gcgaccagcg
    10261 cgctcgagcg ctcggcctac tcgagcgtgc gcgccggcgc cggcgtgtcg atgaagccgg
    10321 tcgccttcac gcgcgtgaag ccgggcgagt cgctccttac ccgaggtgct tactgaagaa
    10381 ctcgagcgtg cggctacgcg cgagcttcga cgcctcggcg ttgaacgaag gccggtgatc
    10441 gcagttgaag ccgtgcccgg cgccggggta gtagtgcgcc gtgatctccg gatgcgcctt
    10501 ggcgatctgc ttggcctgct cgaggctcgg cgtctggtcc tgctcgccga agtggcacat
    10561 cgtcgggcac ttgggcttct cgccgatgaa ccccggcatg ccgccgccgt aatagggcac
    10621 tgcgcacgcc aggccggaaa ggcgcgccgc cgccacccag gtcacggtgc cgccccagca
    10681 gtagccgacg atgccgacct tgccgcccgc cttcgccgct tcgagcgccg ctgccgcgtc
    10741 ctgcatggtc tgcttccagt ctgccttttg catcagggcg cgacccgctt cgatctcggg
    10801 cggcgagtag ccggtgtcgt agccgcgctg catgcggtcg aacatcgacg gcgcgatgac
    10861 gaggtagccg tccgccgcgt agccgtcggc gaccgccttg atgtgcttgt tgacgccgaa
    10921 gatctcctgg atcacgacca cggcgccgcg cggcttgccc gacggctcgg cgcgatacgc
    10981 ggcgagcttg tggccgtcgg ccgccttcag ttcgatcatg cttcccatgc gtcctcctcc
    11041 tgaaaagcct gcggttagaa tcgacattat gaagaagaat gcgcgcgaac tgttccattt
    11101 ggcgaagcag gcgctgatga acgcgggcgc gcatgaaacg atggccgaca ccgcggcgca
    11161 gcatctggtg cgcgccgagg agcagggcct gcccacgcac gg
//
LOCUS       DBNHKE010000123        44416 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_73370, whole genome shotgun sequence.
ACCESSION   DBNHKE010000123 DBNHKE010000000
VERSION     DBNHKE010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 44416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 44416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_73370"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6031)
                     /locus_tag="AC16O9_07760"
     CDS             complement(<1..6031)
                     /locus_tag="AC16O9_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HIR6516550.1"
                     /translation="MKSAILRAFGRAPPAVPWNSEESIREELFGVERLEEHAESLAAA
                     QRVTARPITGRSLTARLKDNETVLLDAYRAIARAVGEGRAVTPAAEWLLDNYPLVEEQ
                     IREIRGDLPPGYYRQLPKLAGGPFAGYPRVFGVAWAFVAHTDSRFDPAMLRRFVRAYQ
                     RVQPLTIGELWAVAITLRIVLVENLRRGARRIVTSRTARQEADALADRLLGAEAPAAG
                     QIAQLLERHGAAPLPESFAVQLVQRLRDQDPKATPVLAWLEERLAAEGKNVEQIVHDE
                     HRRQGATNVTVRNVITSMRLISAVDWAEMVESVSLIDDTLRPASGFAAMDFATRNLYR
                     SAIEELARGARRPELEIAQAALAAAAAAGTAREKDPGYHLIARGRRAFEASIGYRAPV
                     RSWPGRFSRALGIGGYVGVVAAVAALVLALPWFALARAGLGGAWLGVLAALGLVPSLD
                     AAMALVNRWVTRGFGATLLPGLELRDGIPSHLRTLVAVPILLTTRDALEEQIERLEIH
                     YLASPERELHFALLSDWTDAATETAAEDGALLDAAAAGIARLNRRHGAAAAGERFLLL
                     HRRRVWNQGQQCWMGWERKRGKLHELNRLLRGAADTTFIGPSAPISGVRYVITLDADT
                     RMPREAARRLVGKMAHPLNQPRFDAGTRRVVEGYGVLQPRVTPSLPVGREGSLFQRIF
                     SSVGGIDPYAAAVSDVYQDLFGEGSYTGKGIYDVDAFEAALENRVRDGTMLSHDLFEG
                     TFARAGLASDIEVVEEFPSRYDVAAARQHRWARGDWQLLPWIAGRAGALPPIGRWKML
                     DNLRRSLSAPACALALAAGWLLPLYAAVLWTGFIVSTIALSTLLPLLSAIVPARARTT
                     ARSHLGALAADLRLALSLTALLLAFLAHQAWLMSDAIGRTLVRLLVSRRRLLDWVTAG
                     QATVSPRLELPGFYRHMSGGVALGALAALALGWAGSEAWPVAAPFVLAWLAAPAIARW
                     TSLSPLVAGRVPVSPADARALRRVARRTWRFFETFVTAEEHWLPPDNFQEDPKPVVAH
                     RTSPTNLGVYLLSAVSARDFGWAGAAETVERLEATLGTMRGLARFRGHFYNWYDTRDL
                     RPLEPHYVSSVDSGNLAGHLLALANTCREWRHSMHAPDAGDAQALAGIADALELTREA
                     VHALPEDRRTQTITRAQLSEALDALDAALSRGIAHSAPQAATMLDIARTLASERGDEA
                     GAEMLYWAEATHRSIESRWRDHARTGEQARTLQQRLAALEAAARSLAGAMDFAFLLDP
                     ERRLLSIGYRVAEGELDPSCYDLLASEARLASFVAIAKGDVAARHWFRLGRAVTPVSG
                     GAALISWSGSMFEYLMPSLVMRAPAGSLLEQTNRLVVRRQIAYGAGLGLPWGVSESGY
                     HVRDLEFTYQYSNFGIPGLGLKRGLGENAVVAPYATALAAMVDPRAAARNFARLAALG
                     ACGRYGFYEALDYTRARLPEGREVAIVRAFMAHHQGMTVVAIANCLLDGAMRARFHAE
                     PVVRATELLLQERTPRDVAVAHPRVEEAGTAAAVRELELPAVRRLRSAHGATPQAHLL
                     SNGRYAVMLTAAGSGYSRWGEAAITRWREDATRDDWGAYVYLRDAASGEVWSAGHQPT
                     GAEAERTEVSFSDDRAAFVRHDGALATTLEVVVSPEDDAEVRRVSISNSGSEAREIDL
                     TSYAELVLAPLAADAAHPAFSKLFVETEYLAKPGILLATRRRRAPGEPEIWAAHHAVV
                     EGEALGEPEIETDRARFLGRGREARTPMAVVDGLPLSNTVGAVLDPVFALRRRLRVAP
                     GKTARIAFWTVVASSRSDVLDLADKHHDPTAFERAATLAWTHAQVQSSHLGIAPEEAS
                     LFQRLAGHVLYADASLRPSAETLRRGAGAPQLLWAQGISGDLPIVLVRIDDLEDLAIV
                     RQLLRAHEYWRMKQLAVDLVILNERASSYIQDLQIALETQVRAGRTRAAGPAQGARGA
                     VFVLRADLIPAQTRELLLAMARAVLAARRGTLQEQLNGLPAAEAVAPPPRRV"
     gene            complement(6118..6588)
                     /locus_tag="AC16O9_07765"
     CDS             complement(6118..6588)
                     /locus_tag="AC16O9_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015060.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR6516551.1"
                     /translation="MPRARDYVGLAVFVGINAAVSALGGWATSSSVTTWYPTLAKPAF
                     NPPDWVFAPVWSALYLMIAIAGWRAWRRGAAAHPALLVYAVQLALNLSWSFVFFGARL
                     IGPALFVIAALLVAIVVNAALFWRLDRPAGALLVPYAAWVSFAAVLNAALWRLN"
     gene            complement(6590..6988)
                     /locus_tag="AC16O9_07770"
     CDS             complement(6590..6988)
                     /locus_tag="AC16O9_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6516552.1"
                     /translation="MPRTKVEVPNVAEAGPGLWTNGIRAGDFLYISGQVARPFDGAQA
                     LVGTTEYEQAKQIFARIQRICEAAGGGMADVVKVTIFLVDITKNTEVWKARREFFTGD
                     FPASTLVEVRSLAGKETLVEIEAVAYLGKK"
     gene            complement(7004..7348)
                     /locus_tag="AC16O9_07775"
     CDS             complement(7004..7348)
                     /locus_tag="AC16O9_07775"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: protein motif:HMM:NF014043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR6516553.1"
                     /translation="MKPEERDAAYVWDLLRAARLARELLEGVTREAYLRERMRQLALE
                     RALELAGEAARRVSDAFRAAHPEVDWRSLVGQRNVLAHEYGVIDQERLYRTGKESVPG
                     LIAALERILERL"
     gene            complement(7338..7967)
                     /locus_tag="AC16O9_07780"
     CDS             complement(7338..7967)
                     /locus_tag="AC16O9_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR6516554.1"
                     /translation="MGRTRKGIAEALLGRTQQAVLGLLFSRAERGFTLAEIVREARKG
                     TGAVHRELTRLVGAGLVTATPAGRGKRYQANRASPVFGELAGLVAKISGAAVAPAAHS
                     PAAAYAAAPLPAVAPRRLEALCRKYHVRRLGLFGSAARGELAADSDIDLLVEFEPGKA
                     PSFWAEPGMRAAFSALFAGRRVDLVPPAVLENPYRRKGILRDLKVLYEA"
     gene            complement(8022..8795)
                     /locus_tag="AC16O9_07785"
     CDS             complement(8022..8795)
                     /locus_tag="AC16O9_07785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516555.1"
                     /translation="MDLGLKGKSALVTGATQGIGRATALLLAREGARVALVARGLEGL
                     ERCAGEIEAAGGKALPLQADVTRVADLEKAVKTAGERWGGLDILVNNAGTSMRGEFEK
                     VDDAMWQQDLDLKLMAAVRLCRLVLPQMKKRGGGRIVNVTTVGGKQPGATQMPTAVTR
                     AAGLALTKALSKEYAAFNILVNTVCIGRIKAGQWETRAKREGVAVEKLYDSMRGDIPL
                     GRVGEAEEVANVIAFLVSGAASYVTGTSVNLDGGMSGVL"
     gene            complement(9023..9925)
                     /locus_tag="AC16O9_07790"
     CDS             complement(9023..9925)
                     /locus_tag="AC16O9_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR6516556.1"
                     /translation="MSRKHRRRQGSAPETAHRAEAPAARGPHALDNWLLGLAAAGIAL
                     TTYLTFVAWFGAQPAFCGAESDCSLVQQSRWATLLGMPIALWGLATYALLARLLWRLR
                     TRPSAWRMTLTVAGVGAAVSWYLTSVSVLVIEATCAWCLASFAIANALFVLVLLRRPA
                     HMPENAWGKSLPAPAGAAAVIVLGLLLHYSGLFDPAAGPEKPYLKALAVHLSDSGARF
                     YGAYWCPSCQKQKELFEASVERLPYVECTPDGRNGLRNLDCVTNDINDYPTWVIDGRR
                     YTGVLPVANLAALSGFKAPDEGAR"
     gene            complement(9933..10364)
                     /locus_tag="AC16O9_07795"
     CDS             complement(9933..10364)
                     /locus_tag="AC16O9_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6516557.1"
                     /translation="MATVAQILKSKADQVVHKISPTASVFDAVKLMADKNIGAVLVGE
                     GADIAGIFTERDYARKIVLMSKSSKETPVREAMTAQVRYVRPEQTSEECMALMTENRV
                     RHLPVMAEGKLVGLVSIGDLVKDVISEQKFLIEQLEQYITR"
     gene            complement(10787..11971)
                     /locus_tag="AC16O9_07800"
     CDS             complement(10787..11971)
                     /locus_tag="AC16O9_07800"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR6516558.1"
                     /translation="MDAFLFAGMVDLPWWGYVLAALALTHVTIVAVTIFLHRAQAHRA
                     LDLHPAASHFFRFWLWLTTGMVTREWVAVHRKHHAKCDTPEDPHSPRFYGIARVLFGG
                     VFLYVTAARDAHALERYGQGTPEDWLERHLYSKYQSLGIILMGMIDVLVFGLVPGALI
                     LIVQIIWIPFWAAGVINGAGHYWGYRHWQTPDWSTNIAPWGILIGGEELHNNHHAFAT
                     SAKLSSKWYEIDLGWGYIRVLEALGLAKVKHVAPKPRFAAPRPAIDPQTLQAVITNRY
                     DVLAKYARSLWQTYAEEVRLLRRSAPADAYALETVKPLLDRDEKILGDAERRRLAETL
                     PKSSALQTVYAMRRELAALWGRSVASRDQLVAQLQDWCQRAEASGIRSLEEFSLRLRS
                     YA"
     gene            12185..12742
                     /locus_tag="AC16O9_07805"
     CDS             12185..12742
                     /locus_tag="AC16O9_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR6516559.1"
                     /translation="MLQIIVQGRGGQGAQLAGTLLAAAFFAEGREVQSFSTYGGARRG
                     TPVSSFIRVDDKPIRLRCDIEQADALLCFDASLLEGKFLATATPRTLIVVNSRLDAEH
                     FDTVLPGYRTLPVDGLEIAQRNGLGRRVNSALLGALACAAGAPKLETLTATITAQAPK
                     YAHENAAACVDGYGRIAAQLQKAAA"
     gene            12739..14328
                     /locus_tag="AC16O9_07810"
     CDS             12739..14328
                     /locus_tag="AC16O9_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6516560.1"
                     /translation="MNPVAPPPTTWTTGTTAAIKTGTWRAALPAYQTAPSPCHLACPV
                     GGDIARWMQQARQGDWHGAWTTLVEHNPFPAVAGWVCHHPCEQACNRAEYDGALAICA
                     LERHLGERALAAGWRFPAAPLGEQRVAIVGGGPSGLSAAYQLRRRGYAVTIFEARPAL
                     GGLLRYGIPPYRLPRAVLDGEIARLLELGLEVRAGAAIESEAEIMRLRLDYDALYLAP
                     GAAVPKRLAQLDYAAPWVIDGAAYLEQANAGRPPALGQRIAVIGGGSAAMDVARSARR
                     GGHEVIVVSLEAEAQLPAQREEVLAAIDEGVRLLHGAMLRSAERVGERLRLNCVRVKF
                     DSGEVELVPGAELCLYADAIVTAIGHDPQLAPLHWLTPKAGALIRTDALGATTSAGVY
                     AGGDAASLQRYVTHAVGMGRRSALEIDRWLMGELAPLRSRRAPASLAAINTFYHPRSP
                     RSAGGRTAVAAQAEALAEAARCFSCGTCTSCDNCFDACPDMAVRRVNGGYAIDGNYCK
                     GCGLCVAECPTGAIVMREELR"
     gene            14325..15476
                     /locus_tag="AC16O9_07815"
     CDS             14325..15476
                     /locus_tag="AC16O9_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6516561.1"
                     /translation="MSPAQRALLTGNHAVAWGARLARPQVIPVYPITPQTPVLELLTQ
                     FHAAGELDAEFLTPESEHSVMAACIPASLAGARVFTATASQGLMLMHELLHYASGARA
                     PIVMANVNRTVASPWAFWPDQTDSLAQRDTGWVQLYVESAQEALDTVLQAFRVAEAVS
                     LPVMLNLDAFYVSHALEPVSVPPQALVDDYLPAYAPEHLLDPERGESWGNVVTQDMYC
                     RHRRAIGAAMARVPELAAAADREWRERTGRGWGVLERYRCEDAELVIVTLGSMCGTAR
                     EAVDALRASGLAAGLLKVRLFRPLPAAELRAALAGVRDVLVLERDFSPGAGGVLHQEL
                     RAALYGMKDAPGVHGVLAGVGGVNVSAAMIAELARRARIEDAEPEAAWA"
     gene            15467..16321
                     /locus_tag="AC16O9_07820"
     CDS             15467..16321
                     /locus_tag="AC16O9_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6516562.1"
                     /translation="MGVMTDLLCPGHAACPGCVEALSLRHVLAALGPDTVAVIPPSCL
                     AIMAGPQPLSGLRIPVYQPALATAAASASGLRRALDARGKKDTLVTVFAGDGGTYDIG
                     FQALSSAAERNEDILYVCFDNEGYMNTGAQKSSSTPHYARTGSTPAGKVSKKKNLTAI
                     MAAHEVPYAATACAGYRADLLRKVEKAKALRGFRLITLLVPCLDGWGVPEDQGLALSR
                     LAVESGVFPLYEVEDGHRYTLNFPHKSRPVRDYLERQRRYRELDEAAVATLQREVDEG
                     WARLLARL"
     gene            complement(16327..17604)
                     /locus_tag="AC16O9_07825"
     CDS             complement(16327..17604)
                     /locus_tag="AC16O9_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6516563.1"
                     /translation="MNLRRRLVEALALRLPFYYGWVILACVCAAGFSRTGTAVATLSI
                     FIDPMTREFGWSHTEISAAVSAGGILGALVAPATGAFLDRNGARAVLCLAVLGTGIPL
                     LLLSFTQSLAFFFTLYCVARMSFAGPYDLGIYGSIVNWFVRRRAFATSIATLSAMAGL
                     VTMPLIAHFVMQASDWRSAWIAVGATVLLVGFVPTWVLHVRRPEDIGLRPDGERAPAA
                     GESAAPATEPAFTRAEALRTPAFWLLSLFTLLVYPVQAGISLHQAPFLIERGLDPTVA
                     ATAVSTFALLSALAGFASGFWPRRVPLRYALALVGLVLAASSGLMNAVHAAPLAYGAA
                     ALFGLGIGGLLTLLPIAWADYFGRASYGAIRGVALTVQVVAQASGPVLSGALRDWTGS
                     YSASLATFAVLAFAGSAAALFAAPPARRAVRAA"
     gene            17703..18500
                     /locus_tag="AC16O9_07830"
     CDS             17703..18500
                     /locus_tag="AC16O9_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR6516564.1"
                     /translation="MAVPGATDALSARLVEKHGFEAVYIGSYATAASRYGLPDTGLLT
                     LDELVGQARTLVNAVAIPVIADAEGGFNDPANMWRTVQAFEQAGVAAIHIEDHEGPGK
                     HTKLPQKLRAPEAAAARIRAAVQARKDPNLLIAARTDALWIGGELGDAVRRLEAYAKA
                     GADLVFPTLATPAQLAEIRRRVGKPLMIVDLPEGTLADEEKAGASIVLYYGLATLVQF
                     DALQRALETFRRTRDANAVTGYRERIAELEDFLGYKDYVERAKRLGS"
     gene            18500..19744
                     /locus_tag="AC16O9_07835"
     CDS             18500..19744
                     /locus_tag="AC16O9_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6516565.1"
                     /translation="MAALDTAAALITLAALFSWANHRFIGLPSTIALLLFSLAASLGV
                     VALGHLGIHFDTLALELLAEIDFDKALMHGMLSFLLFAGALHVDLDELAERAGAIGFL
                     ATAGVLISTALIGLASWWLFAALGHPVPFLWCLVFGALISPTDPIAVLAIMKSAGTEA
                     SLRIKMTGESLLNDGVGVVVFLVLLGLAAGGADATPAGAAILFVREALGGALFGLAIG
                     WAAYRMLKSVDNYQVEVLVTLALVMGGYALATALHLSGPIAIVVAGLLIGNHGRRFAM
                     SAKTTENLDTFWELIDEIMNAVLFVLIGLEVLLITFDTEFGWAAVLVIPVVLAARLAA
                     VSLAALMPWLRHEFPPRVIGLLTWGGLRGGISIALALSLPAGPERGALITVTYIVVVF
                     SILVQGLTLSRLLRRASPAPSH"
     gene            19761..20159
                     /locus_tag="AC16O9_07840"
     CDS             19761..20159
                     /locus_tag="AC16O9_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6516566.1"
                     /translation="MAKSPKRSKAYTEGLKIRRRWLGSAYVDKAFKDADDFTIDLQHY
                     VTEHGWGASWARGVLPLKTRSIMNLAMISALNRPHELEIHLRAALRNGITKREIKEIF
                     IHVATYCGAPAALDSFRIAKKILAEKPARA"
     gene            20156..21028
                     /locus_tag="AC16O9_07845"
     CDS             20156..21028
                     /locus_tag="AC16O9_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6516567.1"
                     /translation="MTQLRIGAAVAARIEESYEPNFRASKFFPDWDEAVAKEHAAWLA
                     PDHYDPGSGLLKLSVHSWLLTIDGKRILIDACVGNHKPREKRPFWHMMNTPYLERLAA
                     AGAKPEQIDMVMCTHLHVDHIGWNTRLENGRWVPTFPNARYVWSREDFEHYLAIDRDA
                     EKGPANLGSFRDSVLPVVEAGLAQMVSGAAALDESLSVAPAPGHTPGTIAIKFESRGQ
                     KALFCGDILHHALQVYHPEWNSFACADAHNARASRRKALEDCAAGALLMPAHFGAPFV
                     CRIEAKGGGFRPRF"
     gene            complement(21025..21534)
                     /locus_tag="AC16O9_07850"
     CDS             complement(21025..21534)
                     /locus_tag="AC16O9_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516568.1"
                     /translation="MRHFAIAGLACALAACATPQEAPTDPMGRAVTAPLTDLNLLRAK
                     IPPILVSALDAPYAPPADGSCAGLAGEVALLDAELGADLDAAADSGPKPSVIERGIDT
                     VGDAAVGTVRSAAEGVVPMRSWVRRLSGAERHSRQVAAAAAAGVVRRSFLKGLGAAAG
                     CEPPAGPRR"
     gene            21621..22523
                     /locus_tag="AC16O9_07855"
     CDS             21621..22523
                     /locus_tag="AC16O9_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6516569.1"
                     /translation="MKTWTRREFATGAAALGAAGLLPARAVAQDLLTRAIPKSGERLP
                     AVGLGTSRVFNVGDDAEKRAALAEVLRNLVAGGGKIVDTATSYGSSEAVVGDLVARAG
                     LRAQVFIATKLRASDAAELRGSLERLRTQQVDLLFLHNVRDPAQDLGQFRDWKAARAC
                     RYYGVTSTYEGDYPAVEAVLKREKPDFIEIDYSVDNREVEQRLLPLAAETGAAVLIAL
                     PFGRARLFRAVRGKSLPEWARAFGAASWAQFFLKFVLAHPAVTAAIPGTSDPAHMTDN
                     LGALRGRLPDAAERKRMVAFIESL"
     gene            22535..23233
                     /locus_tag="AC16O9_07860"
     CDS             22535..23233
                     /locus_tag="AC16O9_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR6516570.1"
                     /translation="MERRGFLQFAALLAALPLAARAAQPLKIGTLGAGRIGSTLGELW
                     VKAGHPVMFSSRHPEELKGLVTSLGPLAQAGTVADAIAFADVVLLAVPYRALPEIGKA
                     HAGALAQKAAVIDACNPFPHRDGEIAVWAREKGAGLATQALLPGAKLVRAFNAISYAR
                     MAQIGRGREGVGMPIAGEDAGALEVAATLIREAGFEPVRVGGLAMGRHLVPGTALAGE
                     HSPAEIRRIAATLQ"
     gene            23549..24289
                     /gene="trxA"
                     /locus_tag="AC16O9_07865"
     CDS             23549..24289
                     /gene="trxA"
                     /locus_tag="AC16O9_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR6516571.1"
                     /translation="MDITTQTFEAEVLEASRTLPVVADFWAPWCGPCRALTPTLEKVA
                     ADYAGQVKLVKINSDDNQELAAALNIRSIPNVIAFKDGKPVAQFMGAVPESQVRAFFD
                     KLLPSPSELALAAAEALYQAGKLDAAEAKLAEVKFDPDWDDKVEALKQGIAYARQGEG
                     GPGEKELKARLVKDPADHDTRAALAALYAGSKRYGEAMDELLEITRKDKDWKDGDAKR
                     QLLALFSLAADQPELVSAYRKKLAQSLY"
     regulatory      24327..24399
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            24458..25867
                     /gene="ahcY"
                     /locus_tag="AC16O9_07870"
     CDS             24458..25867
                     /gene="ahcY"
                     /locus_tag="AC16O9_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR6516572.1"
                     /translation="MNAPNPKFTDYKVADLSLADWGRKEIAIAESEMPALMAIREEYA
                     KQQPLKGARITGSLHMTIQTAVLIDTLKALGAEVRWASCNIFSTQDHAAAAVAASGTP
                     VFAYKGESLDEYWDFTHRIFEFGAQGPNMILDDGGDATLLVHLGQKAEKDPAVLAKPA
                     SEEETSLFKAIQKKLAKEPGWYTRVAKEIKGVTEETTTGVHRLYEMAKEKRLLFPAIN
                     VNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVAGYGDVGKGSAQALRALSA
                     QVWVTEIDPICALQASMEGYRVVTMDWAADKADIFVTATGNYHVITHEHMAKMKDQAI
                     VCNIGHFDNEIDVAALDKHTWEEVKPQVDHVIFPDGKRIILLAKGRLVNLGCATGHPS
                     YVMSSSFANQTIAQIELFTHTAKYPVGVYTLPKHLDEKVARLQLKKLNAQLTELTEKQ
                     AAYIGVPKHGPYKSDHYRY"
     gene            25871..26272
                     /locus_tag="AC16O9_07875"
     CDS             25871..26272
                     /locus_tag="AC16O9_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6516573.1"
                     /translation="MRRAVLLASLLVVWLINAVALLTVAYVVPGITVADFAAALVAAV
                     VLALVNTIIRPILILLTLPATLVTLGLFIFVINGLLFWLVGSYLEGFEVAGFWSGVLG
                     AIVYSIVSWALAAVLLPERRVRVTVHRVDRP"
     gene            26269..27186
                     /locus_tag="AC16O9_07880"
     CDS             26269..27186
                     /locus_tag="AC16O9_07880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6516574.1"
                     /translation="MRIAVMGAGGVGGYFGARLAQAGHEVSFVARGKHLEAIRAKGLS
                     LKSPLGDATLKVKASDRPAELGDAEIVLFAVKLWDTESAAEAIRPLVEKGGVVIPFQN
                     GVESIERIAKVLGAERVMGGAAYIAGRIGEPGVIVQTGTMARLRFGPVLPAQKKTAEA
                     FLAACKGAKIDAELTDDIVRVLWEKFVLLVAVSATTTVTRGNLGVVRADPDLKWLLEA
                     CMRETWALGRKKGVKLADDLVEQTLKMVDGLPAEMRASMAADLEAGGKLEAPWLSGAV
                     ARMSREAGLEAPANRTVFAALKPCVEGRA"
     gene            27186..28022
                     /gene="metF"
                     /locus_tag="AC16O9_07885"
     CDS             27186..28022
                     /gene="metF"
                     /locus_tag="AC16O9_07885"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR6516575.1"
                     /translation="MAAGERVFSFEFFPPRTPEGKAKLRATWEALARLNPRFFSCTFG
                     AGGSTRAGTLETVLEIRKAGHEAAPHISCVASTRQDIAEQLERYKAHGIRHIVALRGD
                     LPSGIAASGEFRYASELVAFVRESTGDWFRIEVACYPEYHPQTRNAADEIVNFKKKVD
                     AGANAAITQYFYNADAYFRFLEECRAAGIGVPIVPGIMPIGNFSQLARFSDACGAEIP
                     RWMRVKLEGYRDDSASIRAFGLDVVSALCEKLLAAGAPGLHFYTLNQAALSTEIWRRL
                     KL"
     gene            28019..29839
                     /locus_tag="AC16O9_07890"
     CDS             28019..29839
                     /locus_tag="AC16O9_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR6516576.1"
                     /translation="MKRLFAAFAAAALALAALEVFLAHGLQPLEHRLLDAFVRWHAAR
                     LAPDPAIVIIDIDEKSLAQMQDAAGRFPWPRVVYAELLEGLMAQKPRAVVFDLLFSEK
                     DRFRPESDRAFIEAALPHRNVFFPMLRLDPRDDANGASAAELAPLVGLVRRPDADPQT
                     RIAVVPPLALPAELWRGGPINFLEDADGVGRRYLLRTVVGGWALPSLPARVALDLGFG
                     VPDADDLILAWRGTPRGLPRASFADVYQDFGRARRERPADEFTGKIVVIGAAAPGLGD
                     LRVTPLSATQPGVEMLATAIENLQNGRAMQRAPQWLPFGTALLLIGALFAAFARNVDA
                     SRSALALGAASALALGASWLAAGTLLLVPLLAPLAAAWLYYFAAALAAYLRERRARQE
                     TVAMFSRFVNPHVVRALLERGGLEGAGQTREVTLLFSDIRGFTTLSETRAPEEVVAIL
                     NRYFTRQVEAVFRHGGSLDKFIGDAIMAFWGAPLDDPEHARHAVACALDMADELAAFR
                     EELGEAGAGFDVGIGLHSGPAVVGLIGSDKRREYTSIGDTVNLASRIEGLTKDAGRRI
                     LVSRQTMERCQGAFDFVSCGTFPVKGRAQPVELFEPRRRP"
     gene            29836..30321
                     /locus_tag="AC16O9_07895"
     CDS             29836..30321
                     /locus_tag="AC16O9_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR6516577.1"
                     /translation="MKAVLLALLFPPLALAAPATVIRAVDLKQAPASDAATVAALAEQ
                     SGVEVLERKGGWTRVRTAAGAEGWLRMLALRYAATGDAKQGDSGIVQLLNVARTGTSG
                     TQVATGVRGLDPEQLRNAKPNAAELKRLQDYRASPDAAAAFAERGRLQPKQVAYPKEG
                     G"
     gene            30318..31145
                     /locus_tag="AC16O9_07900"
     CDS             30318..31145
                     /locus_tag="AC16O9_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6516578.1"
                     /translation="MRHAATLLAALALAAPAQAQFNINLNRLLDAGKNVRQAAGEIDE
                     KEEIELGGDVAARLLGAAPLVPNAALQTYVNRVGRWLASQTERPNLPWQFGVLDAPQV
                     NAFATPGGHIFVTRGLLARMKNEAELAGVLAHEIAHVLKKHHLKAIQKNAQLELAGTA
                     LSLSRRTEDADKRAKLLAAGSELYLRGLDKSDELEADRLGVVIAARGGYDAYGLPAVL
                     QTLQAMNPEDSGLALMFKTHPAPAERLDALERLQPVLDAHAAQPQLAERFVGAVSGR"
     gene            31158..31796
                     /locus_tag="AC16O9_07905"
     CDS             31158..31796
                     /locus_tag="AC16O9_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR6516579.1"
                     /translation="MKKFLLLATWLACGPALAQHGTGWYIGGGVGTTKADFVRSDFTG
                     TGLATGTYSADDDDVGSRFFGGYRLAPNLAIEGGLAFLGSYKHRFNNGGNVAVYDYSA
                     SALTFALAGNVPVAGGLSLNGRIGIAFAGSELRLRRNNGTAIVPFCPDSWWYTDCASQ
                     STNLYWGVGAQFDVSRNWGIRLDYDNYGEVGEEFETGRADIEQVSVNFVWRF"
     gene            complement(31793..35473)
                     /locus_tag="AC16O9_07910"
     CDS             complement(31793..35473)
                     /locus_tag="AC16O9_07910"
                     /inference="COORDINATES: protein motif:HMM:NF042738.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7933 domain-containing protein"
                     /protein_id="HIR6516580.1"
                     /translation="MRFLRIPVVVLSLLAAPSVLADLTGATVSFSPASIGGGGTSTLT
                     ITLANSPPGNPDNISFTANYPANLFNHTTPSVATDCSNGIVSAAPLGSSVSLSGARIA
                     GNNSCTVTVTVFSCDAGSYPVGGFPVTSTAGTVTASSGTLTVTGNSPASASQSSVTAA
                     PASVPADGSSSATITVTVRTPCGTPLSGKTVTLAQTGSSTITPASAVTNASGVATFTV
                     RSTAPQTDTYTATVTTDSVVITQTTAVTFTQLTAPNVTKGFSPTATGTGLSSTLSITL
                     TNPNAAGSIVGLAFTDTYPGTLVNAATPGLSNTCGGTATGNAGANQLTLTGGTLAAGA
                     SCTVSVSVTSATAGLFNNSSGTVTSTNAANGAASAATLRINNRPTVTKSFSPASVGVG
                     LPSTLTVTISNSNTVAITGAAFTDTYPGALVNHSTPALSNTCGGTATGNAGVGQLTLS
                     GGTVPASGSCAVSVSVNSPSAGSFNNSTGTVTSTNAGSGTAASATLTVNGAPGVAKSF
                     SPASIGTGQTSTLTITLTNTNTVAITGVAFTDSYPANLVNAAVPGLTNSCGGAASAAA
                     GGTQLTLNGGTIPASSSCAVSVSVTSSVAASYNNGTGVVSTTNAGNGGPTFAILTVGV
                     SVSSFNVVEPGGDAVTGRIFTKIAGQDIAVDIVARDASNNIATSFTGVVAVELVDNTS
                     GGACAGLPLIKALANQTFTVGDAGRHLLSAGQFEANAWRNVLFRVKYPTAAPTVTSCS
                     SDAFANRPLQFVSVLARDANRTTAGTTRTLNNTSNPGTGTVHNAGRPFRIDATAQNGA
                     GTPATTTLYSPAAGQPVVILSQCGAGAVCPAALGTLTPGAWSAAAGVITTSTASYGDV
                     GAFSLVLQDQTFSSVDNGDGTATSVRYISSAPVTAGRFVPDHFTLSATSLAPRTDIGA
                     CAGSSFTYMDERMNLGFTLTAREAGGAVTPGYSGATLGALVLNSAASYNFGAIDAAAP
                     TPLTARLDLGMIPGIAATWAAGTTGAITAPIAVSRAAAGPDGPYGTLRIGIAPSDPDS
                     VTLLGAALNLDADNDATPERAQVGPATAVRFGRLVLQSLYGPSNRDLPVTLEAQYWDG
                     TSPTPGFTRNTADSCTPFVRSDFALAFAGGANLVACETAMQEANLTLASGRATITMAS
                     PGAGNEGTVRLSANLGSAGGDHCPAIGAPGSELPASDAGKAYLLGKWDDGAAYDDKPS
                     ASAGFGLFGSQPKNFIFLRENY"
     gene            complement(35540..36583)
                     /locus_tag="AC16O9_07915"
     CDS             complement(35540..36583)
                     /locus_tag="AC16O9_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6516581.1"
                     /translation="MDTLTHALSGALLARATAPKDAPPRSLPRRVAAGFFACAAPDLD
                     FVLAFVSPVFYLEQHRGPTHSVILLPLWALLLAWVLAKLLREPRGWRALYGVTALALG
                     AHIVGDLITSFGTLVFSPFSYERYAWGATFIIDLWFTGIIVAGLLASLAWRRSRVPAM
                     AAGAVLVAYVGFQALLKEQALDYAREYAAKQGWSGVELAAHPGAVTPFNWTVFASDEA
                     AHRFAHVNLRRTEPLPPAHDFVGRLNAAFQPLAHARWETRTRYGATEAEQRIAREAWA
                     SPALGFMRWFADKPAFDGLTAGSTCAWFVDLRFLNPGRDWLPFRYGACQERPGGPWRA
                     FERTGDTSRRAVD"
     gene            complement(36645..38144)
                     /locus_tag="AC16O9_07920"
     CDS             complement(36645..38144)
                     /locus_tag="AC16O9_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6516582.1"
                     /translation="MNCAERLVGIFGSQAEVARAFQLDRAVVHNWVKTGYVPVRWAGE
                     VERVTGGRISAVEILNEASAKNPVRVKSRPDDAPFGITAESQAMTQYAPSKRITSFHP
                     PQRTLMGPGPTEIHPRVLTTMSQPAIGYLDPIFVEMMEELKSLLRYVYQTKHPLTFPL
                     SGPGSVGMEFCFVNMVAPGAKVVVCQNGVFGGRMLENVLRCGGTPIVVEDKWGEPVDP
                     QKVEDALKKAKGASVVAFVHAETSTGAQSDAKELTKIAHKHGALVIVDAVTSLAGTPV
                     KVDEWEIDAIYSASQKCLSCTPGLSPVSFSERVVEHVKKRKDKIHSWFMDMNLLLGYW
                     GETTRTYHHTAPTNSLFALHEALLLIREEGLDNCWARHQRHHVALKAGLEAMGLKFLV
                     KEEYQLPQMNAVLCPDGVDEAQVRSRLLSEFGLEIGAGLGPLKGKIWRFGLMGYSCRP
                     DNVMLCLSALGSVLDDMGYPVHVGDAESAAHGAYAQMHAKDAQRKKRAA"
     gene            complement(38172..38360)
                     /locus_tag="AC16O9_07925"
     CDS             complement(38172..38360)
                     /locus_tag="AC16O9_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516583.1"
                     /translation="MSVIQEMLGRLSGVSTLRDRLNDLHATLRDHQKFILEHERRLAR
                     LEAGPDRPSGRPKLRLSK"
     gene            complement(38357..38641)
                     /locus_tag="AC16O9_07930"
     CDS             complement(38357..38641)
                     /locus_tag="AC16O9_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516584.1"
                     /translation="MKAMQSTMACLTPEQKTGLARRAQQRNTKMAAEIRSAVDAYLAG
                     VTPQELELLDAATRHAQAEIAGMVKTVDQIVASHAAFLRDMRRLRKGEAR"
     gene            38726..39358
                     /locus_tag="AC16O9_07935"
     CDS             38726..39358
                     /locus_tag="AC16O9_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR6516585.1"
                     /translation="MPLETHYASGLLLFALLNPFLMSVYLIDLITDLPRAMFVRVLAR
                     GALISALVFAVFAWTGDAIFSDVLHVRFASFQVFGGVIFLLIGVRFVFSGADAMRAMR
                     GHPEHIAGSIAMPFMIGPGTVSASVVVGARLPVPDAVMVIFATLAVTVALVAAIKFTH
                     DYVKERNARVVDRYIEIVGRISALLIGTFAIEMIFEGLGTWLEDTAPQWR"
     gene            complement(39366..40097)
                     /locus_tag="AC16O9_07940"
     CDS             complement(39366..40097)
                     /locus_tag="AC16O9_07940"
                     /inference="COORDINATES: protein motif:HMM:NF023984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HIR6516586.1"
                     /translation="MRRRHVLGAGLVAIVLAIGVGAVTRAGADWRTASREPVGLAPDP
                     AQAAEAVVQVYAARAVGMRGLFGVHSWIAVKPQNAPAYTVYEVIGWKLRWDDTSVSVR
                     TRAPDARWFGNEPELLADVRGAAAAPLIARIEGAVQTYPYGKEYRAWPGPNSNTFTAW
                     VTRAVPELKADLPSTAIGKDYLRGGVFATAPSGSGYQMSLAGLFGITASTVEGLEVNL
                     LGLTFGVDPWPPALKLPLAGRIGAR"
     gene            complement(40094..41191)
                     /gene="gtdA"
                     /locus_tag="AC16O9_07945"
     CDS             complement(40094..41191)
                     /gene="gtdA"
                     /locus_tag="AC16O9_07945"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HIR6516587.1"
                     /translation="MKPVEGARREAEELEGIVPTVNLAAEEGRKDFYARAKKQHLAPL
                     WRVLHGLVTDKPATRAVPAIWHYADVRPHLMEACELIGAAEAERRVMVLENPGLAGQS
                     RITQSLFAGLQIILPGEIAPAHRHTASALRFIVEGSNAFTAVAGERTMMQPGDFVITP
                     SWTWHDHGHEGEGPMVWLDGLDMHIVNMLAASFREDLGGKAHKLERPEGAAPAEFGNN
                     LLPVDAVHRSQTSPIFNYPYRKSRESLDALQRTRDPDAWHAYKMKYINPVNGSWAMPT
                     ISTWLQLLPKGFKTAPYRSTDSTVFAVVEGRGRSTIQGETFEWGPHDIFVAPSWMAHT
                     HQASADAVLFLYSDRVIQEKLDIWRESREKA"
     gene            complement(41178..41999)
                     /gene="ttcA"
                     /locus_tag="AC16O9_07950"
     CDS             complement(41178..41999)
                     /gene="ttcA"
                     /locus_tag="AC16O9_07950"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR6516588.1"
                     /translation="MHVVRLQRDPRKAAYEAGKLAKRLRRQVGEAIADFGMIEAGDRV
                     MVCLSGGKDSYAMLDVLMALREKAPVPFELVAVNLDQKQPGFPAHVLPDYLAGLGVPF
                     RIVEQDTYSVVKRVLPEGKTMCSLCSRLRRGVLYRVAGELGATKIALGHHRDDILATF
                     FLNLFFGGKLKAMPPKLRSDDGRHVLIRPLAYVREDDLAAYAEEKGFPIIPCNLCGSQ
                     EHLQRRVVRDMLRQWEKAHPGRVESILRALGDVRPSHLMDRKLFDFSLGAVDETG"
     gene            complement(42008..42757)
                     /locus_tag="AC16O9_07955"
     CDS             complement(42008..42757)
                     /locus_tag="AC16O9_07955"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HIR6516589.1"
                     /translation="MTDASLESKVALVTGAARRVGAAIARRLHAAGASIVIHYRGAET
                     DAAQLEKDLNVLRRGSAIKVKCDLLAPVAPQALVSAAQQHFARLDFLVNNASAFYPTP
                     LGEIEASHWEELIGSNLRAPLFLAQAAAPQLALAGGAIVNIADIHAERPLKDYVVYSV
                     AKAGLVALTRALALELGPGVRVNAVAPGAIAWPEDGQFAPDERKRIVATTPLARTGAA
                     EDVAQAVHFLCGAPFVTGQVLAVDGGRSIFL"
     gene            42786..43889
                     /locus_tag="AC16O9_07960"
     CDS             42786..43889
                     /locus_tag="AC16O9_07960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516590.1"
                     /translation="MLPEPDAEARAASARLAERIRAEIAAGGGWLGFVRYMELALHAP
                     GLGYYAGGASKLGAAGDFATAPELGTLFARTLARQLAEVLAPGEAILEFGAGSGALAA
                     ALIAELAPERYLILETSAELAARQRARLGARAQWIERLPERFRGVMIANEVADAMPVH
                     ALAWRREGVMERGVRVAGGAFAWDERPATGELREAARRLAVPVPYESEIGLMARAWMK
                     LVAPRLERGALFVIDYGFPEREYYHPQRAMGTLMCHYRHRAHGDVFFHPGLQDITAHV
                     DFTALARGAREAGLEVLGYANQAGFLVDCGITEVLGREDPEDVARYAPRAAEAQMLLS
                     PAEMGELFKVLAVGRGVPRPLLGFANCDRSHTL"
     gene            43894..>44416
                     /locus_tag="AC16O9_07965"
     CDS             43894..>44416
                     /locus_tag="AC16O9_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516591.1"
                     /translation="MRLIAVLALALPLAALGDDIAEAKKRWAESPHGPLLERILPPTF
                     ERAQLPEPRARGARLTVQYCVQCHNLPNPAMHHAEKWPTIVERMVLRMEGKGNYGQLM
                     FDMMAGVKAPRAPETDALVAYLQKHSQKPLDPRKVPEAYTAAGEPFRLACNQCHVLPD
                     PRRYTAKEWPAVVS"
BASE COUNT         6445 a        15947 c        15704 g         6320 t
ORIGIN      
        1 gcacgcgccg cggcggcggc gcgaccgcct ccgccgccgg caggccgttc agctgctcct
       61 gcagcgtgcc gcggcgcgcg gcgagcaccg cgcgcgccat ggcgagcagc agctcgcgcg
      121 tctgcgccgg aatcagatcg gcgcgcagca cgaacaccgc gccgcgcgcg ccctgcgccg
      181 ggcccgcggc gcgcgtgcgg cccgcgcgca cctgcgtctc cagcgcgatc tgcaggtcct
      241 ggatgtacga gctggcgcgc tcgttcagga tcaccaggtc caccgcgagc tgcttcatgc
      301 gccagtactc gtgcgcgcgc agcagctggc gcacgatggc gaggtcctcg aggtcgtcga
      361 tgcgcacgag cacgatcggc aggtcgcccg agatgccctg cgcccagagc agctgcggcg
      421 cgccggcgcc gcggcgtagc gtctcggcgg acgggcgcag cgaggcatcg gcgtacagca
      481 cgtggcccgc gaggcgctgg aacaggctcg cctcctccgg cgcgatgccc aggtggctcg
      541 actgcacctg cgcgtgggtc caggccagcg tggccgcgcg ctcgaaggcc gtcgggtcgt
      601 ggtgcttgtc ggcgagatcc aggacgtcgc tgcgcgagga cgcgaccacc gtccagaagg
      661 cgatgcgcgc cgttttgccc ggcgccacgc gcaggcggcg gcgcagcgcg aacaccggat
      721 cgagcaccgc gccgaccgtg ttggacagcg gcaggccgtc gacgaccgcc atcggcgtgc
      781 gcgcctcgcg cccgcggccg agaaagcgcg cgcggtcggt ttcgatctcc ggctcgccca
      841 gcgcctcgcc ttcgaccacg gcgtggtgcg ccgcccagat ttccggctcg ccgggcgcgc
      901 ggcgccggcg cgtcgcgagc aggatgccgg gcttggcgag gtattcggtt tccacgaaga
      961 gcttggagaa cgcggggtgc gccgcgtcgg cggcgagcgg cgccagcacc agctcggcgt
     1021 aggaggtgag gtcgatctcg cgcgcctcgc tgccggaatt cgagatcgac acgcggcgca
     1081 cttcggcgtc gtcctcgggc gagaccacga cctcgagcgt cgtggcgagc gcgccgtcgt
     1141 gccgcacgaa tgcggcgcga tcgtcgctga agctcacctc ggtgcgctcg gcctcggcgc
     1201 cggtgggctg gtggcccgcc gaccacacct cgccgctcgc cgcgtcgcgc aggtagacgt
     1261 acgcgcccca atcgtcgcgc gtggcgtctt cgcgccagcg cgtgatcgcg gcctcgcccc
     1321 agcggctgta gccggagccg gcggcggtca gcatcaccgc gtagcggccg ttggagagca
     1381 ggtgcgcctg cggcgtggcg ccgtgcgcgg agcgcagccg ccgcaccgcg ggcagctcga
     1441 gctcgcgaac cgcggccgcc gtgccggcct cctccacccg cgggtgcgcg acggcgacat
     1501 cgcgcggcgt gcgctcctgc agcagcagct cggtggcccg caccaccggc tcggcgtgga
     1561 accgcgcccg catcgcgccg tcgaggaggc aattggcgat cgcgaccacc gtcatgccct
     1621 ggtggtgcgc catgaaggcg cgcacgatcg ccacctccct gccctcgggc aggcgcgcgc
     1681 gcgtgtaatc cagcgcctcg tagaagccgt agcggccgca ggcgccgagc gcggcgagcc
     1741 gggcgaagtt gcgcgccgcg gcgcgcgggt cgaccatcgc cgcgagcgcc gtcgcgtagg
     1801 gcgcgacgac ggcgttctcg cccaggccgc gcttcagccc caggcccggg atgccgaagt
     1861 tcgaatactg gtaggtgaac tccaggtcgc gcacgtggta gccggactcc gacacgcccc
     1921 agggcaggcc gagcccggcg ccgtaggcga tctgccggcg caccaccagc cggttggtct
     1981 gctcgagcag gctcccggcg ggcgcgcgca tgacgagcga gggcatcagg tactcgaaca
     2041 tcgatcccga ccaggagatg agcgcggcgc cgccgctgac cggcgtcacg gcgcgcccga
     2101 gccggaacca gtggcgcgcg gcgacgtcgc ccttggcgat cgccacgaag ctcgccaggc
     2161 gcgcttcgga ggcgagcagg tcgtagcagc tcgggtcgag ctcgccctcc gcgacgcggt
     2221 agccgatcga caggagcctt ctctccgggt cgaggagaaa cgcgaaatcc atcgcgccgg
     2281 cgaggctgcg cgccgccgcc tcgagcgccg ccaggcgctg ctgcagcgtg cgcgcctgct
     2341 cgccggtgcg cgcatgatcg cgccagcggc tctcgatcga gcggtgcgtg gcctcggccc
     2401 agtagagcat ctcggcgccg gcttcgtcgc cgcgctcgct cgccagcgtg cgcgcgatgt
     2461 cgagcatggt cgcggcctgc ggtgcgctgt gcgcgatccc ccgggaaagc gcggcgtcca
     2521 gcgcatcgag cgcctcgctg agctgcgcgc gcgtgatggt ctgcgtgcgg cggtcctcgg
     2581 gaagcgcgtg cacggcttcg cgcgtgagct ccagagcgtc ggcgatgccg gccagcgcct
     2641 gcgcgtcgcc ggcgtcgggc gcgtgcatcg agtgccgcca ctcgcggcag gtgttggcca
     2701 gcgcgagcag gtgcccggcg aggttgccgc tgtccaccga cgagacgtag tgcggctcga
     2761 gcggccgcag gtcgcgcgtg tcgtaccagt tgtagaagtg cccgcgaaag cgcgcgaggc
     2821 cgcgcatcgt cccgagcgtc gcctccagcc gctccacggt ctcggcggcg ccggcccagc
     2881 cgaagtcgcg cgcgctgacc gcggacagca gatacacgcc caggttggtg ggcgaggtgc
     2941 ggtgcgcgac gaccggcttg gggtcttcct ggaagttgtc cggcggcagc cagtgctcct
     3001 cggccgtgac gaacgtctcg aagaagcgcc aggtgcggcg cgcgacccgg cgcagcgcgc
     3061 gcgcatccgc gggcgacacc ggcacgcggc cggcaacgag cggcgacagg ctggtccagc
     3121 gggcgatggc gggcgcggcg agccaggcga gcacgaacgg cgccgccacc ggccaggcct
     3181 cgctcccggc ccagcccaga gcgagcgcgg cgagcgcacc gagcgccacg ccgccgctca
     3241 tgtgccggta gaacccgggg agctcgagcc gcgggctcac cgtcgcctgg ccggccgtga
     3301 cccagtcgag cagccgccgg cggctcacga gcagccgaac cagcgtgcgg ccgatcgcgt
     3361 cgctcatcag ccaggcctgg tgcgcgagga acgcgagcag cagcgcggtg agcgacaagg
     3421 cgagccgcag gtccgcggcg agcgcgccca ggtggctgcg cgcggtggtg cgcgcgcgcg
     3481 ccggcacgat cgccgagagc agcggcagca gcgtcgagag cgcgatcgtc gacacgatga
     3541 agccggtcca gaggacggcg gcatacaggg gcagcagcca gccggcggcg agcgcgagag
     3601 cgcacgccgg cgccgacagc gagcggcgca ggttgtccag catcttccag cggccgatcg
     3661 gcggcagcgc gcccgcgcgc ccggcgatcc agggcagcag ctgccagtcg ccgcgcgccc
     3721 agcggtgctg gcgcgcggcg gccacgtcgt agcgcgacgg aaactcctcg accacctcga
     3781 tgtcggaggc gaggccggcg cgcgcgaacg tgccttcgaa caggtcgtgg ctgagcatcg
     3841 tgccgtcgcg cacgcggttc tcgagcgccg cttcgaaggc atccacgtcg tagatgccct
     3901 tgcccgtgta ggagccctcg ccgaagagat cctggtacac gtcggacacc gcggcggcgt
     3961 agggatcgat gccgcccacg ctggagaaga tgcgctggaa cagcgagccc tcgcggccca
     4021 ccggcagcga gggcgtcacg cgcggctgca gcacgccgta gccctcgacg acgcgccgcg
     4081 tgccggcatc gaagcgcggc tggttgagcg ggtgcgccat cttgccgacc aggcgccgcg
     4141 ccgcttcgcg cggcatgcgc gtatcggcgt cgagcgtgat gacgtagcgc acgcccgaaa
     4201 tcggcgcgga ggggccgata aaggtcgtgt cggccgcgcc gcgcagcagc cggttcagct
     4261 cgtgcagctt gccgcgcttg cgctcccagc ccatccagca ctgctgccct tggttccaca
     4321 cgcgccggcg atgcagcagc aggaaccgct cgcccgcggc ggccgctccg tggcggcggt
     4381 tcaggcgcgc gatgcccgcc gccgccgcgt cgagcagcgc gccgtcctcg gccgcggtct
     4441 cggtcgcggc gtcggtccag tccgacagca gcgcgaaatg cagctcgcgc tccgggctgg
     4501 cgaggtaatg gatctccagg cgctcgatct gctcttccag cgcatcccgg gtggtgagca
     4561 ggatcggcac cgcgaccagc gtgcgcaggt gcgaagggat gccgtcgcgc agctccagcc
     4621 ccggcagcag ggtcgcgccg aagccgcgcg tcacccagcg gttcacgagc gccatcgccg
     4681 cgtcgagcga gggcacgagg ccgagcgccg cgagcacgcc gagccaggcg ccgccgagcc
     4741 ccgcgcgcgc cagcgcgaac cacggcaggg cgagcacgag cgcggcgacc gcggcgacca
     4801 cgccgacgta gccgccgatg ccgagcgccc ggctgaagcg cccgggccag ctgcgcacgg
     4861 gggcgcgata gccgatcgac gcctcgaagg cgcggcgccc gcgcgcgatc aggtggtagc
     4921 cgggatcctt ctcgcgcgcg gttccggcgg cggcggcggc ggcgagggcc gcctgcgcga
     4981 tctccagctc cgggcgccgc gcgccgcgcg cgagctcctc gatggcgctg cgatagaggt
     5041 tgcgcgtcgc gaaatccatc gccgcgaagc cgctcgccgg ccgcagcgtg tcgtcgatga
     5101 ggctgacgct ctccaccatc tcggcccagt ccaccgcgga gatcagccgc atgctggtga
     5161 tgacgttgcg caccgtcacg ttcgtcgccc cctggcgccg gtgctcgtcg tgcacgatct
     5221 gctcgacgtt cttgccctcc gccgccaggc gctcctcgag ccacgccagc accggcgtcg
     5281 ccttcggatc ctggtcgcgc aggcgctgca cgagctgcac ggcgaaggac tccggcaggg
     5341 gcgctgcgcc gtggcgctcg aggagctggg cgatctgccc ggccgcgggc gcctccgcgc
     5401 ccagcagccg gtcggccagc gcgtcggctt cctggcgcgc cgtgcggctg gtcacgatgc
     5461 ggcgcgcgcc gcgccgcagg ttctccacca gcacgatgcg cagcgtgatc gccaccgccc
     5521 acagctcgcc gatggtgagc ggctgcacgc gctggtaggc gcgcacgaag cgtcgcagca
     5581 tcgcggggtc gaagcggctg tcggtgtgcg cgacgaacgc ccaggccacg ccgaacacgc
     5641 gcggatagcc ggcgaacggt ccgccggcga gcttcggcag ctgccggtag tagcccggcg
     5701 gcaggtcgcc gcggatctcg cggatctgct cttcgaccag ggggtagttg tcgagcagcc
     5761 actcggcggc cggcgtcacg gcccggccct cgccgaccgc gcgcgcaatg gcgcgatacg
     5821 cgtcgagcaa taccgtctcg ttgtccttca gccgcgcggt gagcgatcgc ccggtgatcg
     5881 gcctcgcggt cacgcgctgc gccgccgcga ggctctcggc gtgctcctcg aggcgctcga
     5941 caccgaagag ctcctcgcgg atcgactctt cgctattcca ggggactgcc ggcggggcgc
     6001 gcccgaaggc gcgaagaatt gctgatttca atatgacctt tgtgggtcag cgcattatcc
     6061 ccccttcggt gcccggcgag gcgaatctcc gcgcgcgcgc tacgcgcggc gctgcggcta
     6121 gttgagccgc cacagcgccg cgttcagcac cgcggcaaac gaaacccagg ccgcatacgg
     6181 cacgagcagc gcgccggccg gccggtcgag gcgccagaag agcgctgcat tgacgacgat
     6241 cgcaacgagc agcgccgcga tcacgaagag cgccggcccg atcaggcgcg cgccgaagaa
     6301 cacgaacgac cacgacaggt tgagcgcgag ctgcaccgcg tacacgagaa gcgcggggtg
     6361 cgccgcggcg ccgcggcgcc acgcgcgcca gcccgcgatc gcgatcatca ggtagagcgc
     6421 gctccacacc ggcgcgaaca cccagtcggg cggattgaac gcgggcttgg cgagcgtcgg
     6481 gtaccaggtc gtgacgctcg acgaggtcgc ccagccgccc agcgccgaaa cggcggcgtt
     6541 gatgccgacg aacaccgcca gcccaacgta gtcccgcgcg cgcggcacgc tacttcttcc
     6601 cgagatacgc caccgcctcg atctccacca gcgtctcctt accggcaagc gagcgcacct
     6661 cgaccagcgt cgaggccgga aaatccccgg tgaagaactc gcgccgcgcc ttccacacct
     6721 cggtgttctt ggtgatgtcc accaggaaga tcgtcacctt gaccacgtcc gccatgcccc
     6781 cgcccgcggc ctcgcagatg cgctggatgc gcgcgaagat ctgcttcgcc tgctcgtact
     6841 cggtcgtgcc gacgagcgcc tgagcgccat cgaacggccg cgccacctgg ccggagatgt
     6901 agagaaaatc ccccgcgcga atgccgttgg tccacaaccc cggaccggct tcagcgacat
     6961 tgggtacctc aaccttggtt ctcggcatag ctccctcctt tgctcaaagc ctctccagga
     7021 tcctctccag cgccgcgatc aggccgggca cgctctcctt cccggttcgg tacaagcgct
     7081 cctggtcgat cacgccgtac tcgtgcgcga ggacgttgcg ctgcccgacg aggctgcgcc
     7141 agtccacttc cggatgggcg gcccggaacg cgtccgacac tcgccgcgcg gcttcgcccg
     7201 cgagctccag tgcgcgctcc agcgcgagct ggcgcatgcg ctcgcgcagg taggcctccc
     7261 gcgtcacgcc ctccagcagc tcgcgcgcca ggcgcgccgc gcgcagcagg tcccacacgt
     7321 acgcggcatc gcgctcctca ggcttcatag agtaccttca ggtcgcgcag gatgcccttc
     7381 ctgcgatacg ggttctccag cacggcgggc ggcacgaggt ccacgcgccg gccggcgaat
     7441 agcgcgctga acgccgcgcg catcccgggc tccgcccaga acgaaggcgc tttgcccggc
     7501 tcgaactcga ccagcaggtc gatgtcgctg tcggccgcga gctcgccgcg cgcggccgag
     7561 ccgaacagcc cgagccggcg cacgtggtat ttgcggcaca gcgcttcgag cctgcgcggc
     7621 gccacagcgg gcagcggcgc cgccgcgtac gccgcggcgg gcgagtgcgc ggccggcgcc
     7681 accgcggcgc cggagatctt ggccaccagg ccggcgagct cgccgaatac gggcgaggcg
     7741 cgattggcct ggtagcgctt tccgcgcccg gccggcgtcg ccgtcaccag tcccgcgccg
     7801 accagccgcg tcagctcgcg gtgcaccgcc ccggttcctt ttcgcgcctc gcgcacgatc
     7861 tcggcgagcg tgaagccccg ctccgcccgg gaaaaaagca gccccagcac cgcctgctgg
     7921 gtgcgcccga ggagcgcctc ggcgatgccc ttgcgcgttc gtcccataat gggacgttat
     7981 cctcccgatc tgggtcgccg tcaagccgcg gcggcggcga cctacaacac ccccgacatc
     8041 ccgccatcca ggttcacgct cgtccccgtg acgtagctcg ccgcgccgga aacgaggaac
     8101 gcgatcacgt tcgccacttc ctcggcctcg cccacgcgcc cgagcggaat gtcgccgcgc
     8161 atcgagtcgt agagcttttc caccgccacg ccttcgcgct tcgcgcgcgt ctcccactgc
     8221 cccgccttga tgcgcccgat gcacacggtg ttcaccagaa tgttgaacgc cgcgtactcc
     8281 ttggaaagcg ccttggtgag cgcaaggccc gcggcgcggg tgacggccgt gggcatctgg
     8341 gtcgcgccgg gctgcttgcc gcccaccgtc gtcacgttga cgatcctccc gccgccgcgc
     8401 ttcttcatct gcggcagcac cagacggcac agccgcaccg cggccatcag cttgagatcg
     8461 agatcctgct gccacatcgc gtcgtcgacc ttctcgaact cgccgcgcat cgaggtgccg
     8521 gcgttgttga cgaggatgtc gagcccgccc cagcgctcgc cggccgtctt caccgccttc
     8581 tcgaggtctg caacgcgcgt gacatccgcc tgcagcggca gcgccttgcc gcccgcggcc
     8641 tcgatctccc ccgcgcagcg ctccaggcct tccagcccgc gcgccaccag cgccacgcgc
     8701 gcgccttcgc gcgccagcaa caaagccgtc gcccggccga tgccctgggt cgcgccggtg
     8761 acgagcgcgc tcttgccctt caatccgaga tccatgccgc cgtccccctc gctcgtgatc
     8821 gacccgcacg gcgatgatag cgccgccgcg cccgcttccg ccgcgtagcc aggccggcac
     8881 gcgaggcgta ggttggcgcg gtcccgaagc ccttccggga ccgtcgatca aaggggaggc
     8941 cgatgaaggc cgagctcagc taccttgcct acaccgcgat gctgaccgcg gcgctgtgga
     9001 gtcaggtaag ccgggcgccg gctcagcgcg cgccctcgtc gggcgccttg aagcccgaca
     9061 gggccgccag attggcgacc ggcagcacgc cggtgtagcg gcggccgtcg atgacccagg
     9121 tcggatagtc gttgatgtcg ttcgtgacgc agtccaggtt gcgcaggccg ttacgcccgt
     9181 ccggcgtgca ctcgacgtag ggcaagcgct cgacggacgc ttcgaacaac tccttctgct
     9241 tctggcagga cgggcaccag tacgcgccgt agaagcgcgc cccggaatcg ctcaggtgca
     9301 cggcgagcgc tttcaggtag ggcttctcgg gcccggcggc gggatcgaac aggccgctgt
     9361 agtgcaggag cagtccgagc acgatcaccg cggccgcgcc cgccggcgcc ggcagcgact
     9421 tgccccacgc gttctccggc atgtgcgcgg gcctgcgcaa cagcaccagg acgaagagcg
     9481 cgttcgcgat cgcgaacgag gcgaggcacc aggcgcaggt cgcctcgatg acgagcaccg
     9541 acaccgaggt gagataccag ctcaccgcgg cgccgacgcc ggcgacggtc agcgtcatgc
     9601 gccaggcgct cgggcgggtg cgcaagcgcc acagcagccg cgcgagcagc gcgtaggtgg
     9661 ccaggcccca cagcgcgatc ggcatgccga gcagcgtggc ccagcggctt tgctgcacga
     9721 ggctgcagtc ggactccgcg ccgcagaacg ccggctgcgc gccgaaccag gcgacgaacg
     9781 tcaggtaggt cgtcagcgcg atgccggcgg cggcaagccc gagcagccag ttgtcgagcg
     9841 cgtgcgggcc gcgcgccgcc ggcgcctcgg cgcggtgcgc cgtctccggg gcggagcctt
     9901 gcctgcggcg gtgttttctc gacatgccgc ccctagcgcg tgatgtactg ctcgagctgc
     9961 tcgatcagga atttctgctc ggagatcacg tccttcacca ggtcgccgat cgacaccagg
    10021 cccacgagct tgccctcggc catcaccggc aggtgccgca cccggttctc ggtcatcagc
    10081 gccatgcatt cctcgctcgt ctgctcgggg cgcacgtacc tcacctgcgc cgtcatggcc
    10141 tcgcgcaccg gcgtttcctt cgacgacttc gacatcagca cgatcttccg cgcgtagtcg
    10201 cgctcggtga agatgccggc gatgtccgcg ccctcgccca ccaggaccgc gccgatgttc
    10261 ttgtcggcca tcagcttgac cgcgtcgaat accgaagccg tgggcgagat cttgtgcacg
    10321 acctgatccg ccttcgactt gagaatctgc gccaccgtcg ccattagtat cctttccagt
    10381 agtggccgct actgctgcag cggcctgccg gtcccgcccc tgcgccgtgc gcgcccgcgg
    10441 gcgcggccac gaggccggcg cacgccagga taaggatcgg cttgcgcact gcccccatat
    10501 gaaaccgacg ctacgcccac tccgaaacgc ggtcaacgca tgaaacgcga aacgctctca
    10561 gtcccgcgag ttcggcccgg cgaaaacccg gcggcggcgt gaacgaatcc tcacgcgcgc
    10621 acgtccaagc aggaaggagg ggtatggtgc attgctggca ccggccgcga gccggcaaca
    10681 tcgagcaatg cggggagcat cacgatgctt cccacccctc cccgcctcgc gcggcggcgt
    10741 cgcggtgtag ccgagccggg tcgacgggca ccgccaggga tcgcgctcag gcgtagcttc
    10801 tcagccgcag cgagaactct tccagcgacc ggatgccgct cgcctccgcc cgctggcacc
    10861 agtcctggag ctgcgcgacg agctggtcgc gcgaggcgac cgagcgcccc cacagcgcgg
    10921 cgagctcgcg gcgcatggcg tacacggtct gcagcgcgct cgacttgggc agcgtctcgg
    10981 cgagccgccg gcgctcggcg tcgcccagga tcttctcgtc gcggtcgagc aacggcttca
    11041 cggtctccag cgcgtaggcg tccgccggcg cgctgcgccg caggagccgc acttcctcgg
    11101 cgtaggtctg ccacagcgag cgcgcgtact tggccagcac gtcgtagcgg ttggtgatca
    11161 cggcctgcag cgtctgcgga tcgatcgccg gccgcggcgc ggcgaagcgc ggcttgggcg
    11221 ccacgtgctt gaccttcgcc agccccagcg cttcgagcac ccggatgtag ccccagccca
    11281 gatcgatctc gtaccacttg ctggagagct tggccgaggt ggcgaaggcg tggtggttgt
    11341 tgtgcagctc ctcgccgccg atcaggatgc cccagggcgc gatgttggtg ctccagtcgg
    11401 gcgtctgcca gtgccgatag ccccagtagt ggcccgcgcc gttgatcacg cccgcggccc
    11461 agaacggaat ccagatgatc tgcacgatca ggatcagcgc gcccggcacg aggccgaaca
    11521 cgaggacgtc gatcatcccc atgaggatga tgccgaggct ctggtacttc gagtacaggt
    11581 ggcgctccag ccagtcctcc ggcgtgccct ggccgtagcg ctcgagcgca tgcgcatccc
    11641 gcgccgccgt cacgtacagg aacacgccgc cgaacagcac gcgcgcgatg ccgtagaagc
    11701 gcgggctgtg cggatcctcg ggcgtatcgc acttggcgtg gtgcttgcgg tgcaccgcga
    11761 cccattcgcg cgtcaccatc cccgtggtca gccacagcca gaaacggaaa aagtggctcg
    11821 ccgccggatg caggtcgagc gcccggtgcg cctgggcgcg gtgcaggaag atcgtcaccg
    11881 cgacgatggt gacgtgggtg agcgcgaggg ccgccagcac gtagccccac cacggcaggt
    11941 cgaccattcc ggcaaagagg aaagcgtcca tgctccccaa gactctagcg cgcttgcggg
    12001 cgcaataagt tgacgccgct caactacgcg tcccggcacg tgagctgcgc atccggcaac
    12061 tccccgccct gcccgggtcc cgccagccgc gcgctgaggg gggaggcccg cccgggcagg
    12121 cgcgcgcttt tcttgatccg cctcaaaccc cccctccccg caaggcgcat gctgggccgc
    12181 gcgcatgctc cagatcatcg tccagggacg tggcggccag ggggcgcagc tcgccgggac
    12241 gctgctcgcc gcggcgttct tcgccgaggg gcgcgaggtg cagagcttct cgacctacgg
    12301 cggcgcgcga cgcggcacgc cggtgagctc cttcatccgc gtggacgaca agccgatccg
    12361 cctgcgctgc gacatcgagc aggcggacgc gctgctgtgc ttcgacgcga gcctgctcga
    12421 aggaaagttc ctcgccaccg cgactccgcg cacgctgatc gtggtgaatt cgcgcctgga
    12481 cgcggagcat ttcgacaccg tgctccccgg ctaccgcacg ctgccggtgg acgggctgga
    12541 gatcgcgcag cgtaacgggc tcggccgcag ggtgaactcg gcgctgctcg gcgcgctcgc
    12601 ctgcgccgcg ggcgcgccga agctcgagac gctcaccgcg acgatcaccg cgcaagcgcc
    12661 gaagtacgcg cacgaaaacg cggcggcctg cgtcgacggc tatggccgca tcgcggcgca
    12721 gctgcaaaag gccgccgcat gaatccggtc gcgccgccgc ccaccacctg gaccaccggc
    12781 accacggcgg cgatcaagac cggcacctgg cgcgcggcgc tgcccgccta ccagacggcg
    12841 ccgtcgccgt gccacctcgc ctgcccggtg ggcggcgaca tcgcgcgctg gatgcagcag
    12901 gcaagacaag gagactggca tggcgcctgg acgacactcg tcgagcacaa tccctttccc
    12961 gcggtggctg ggtgggtctg ccaccatccc tgcgaacagg catgtaatcg cgcggagtat
    13021 gacggcgcgc tcgccatctg cgcgctcgag cgccatctcg gcgagcgcgc gctcgccgcg
    13081 ggctggcgct tcccggcggc gccgctcggc gagcagcgcg tagcgatcgt cggcggcggc
    13141 ccctcgggcc tgtcggcggc ctaccagctg cgccgccgcg gctacgcggt cacgatcttc
    13201 gaagcgcgac cggcgctcgg cgggctgctg cgctacggca tcccgcccta tcggctgccg
    13261 cgcgcggtgc tcgacggcga gatcgcgcgc ctgctcgagc tcggcctgga ggtgcgcgcg
    13321 ggcgccgcaa tcgagagcga agccgagatc atgcgtctgc gcctggacta cgacgcgctg
    13381 tacctcgcgc ccggcgccgc ggtgcccaag cgcctggcgc agctcgacta cgccgcgccg
    13441 tgggtcatcg acggcgccgc ctacctcgag caggcgaacg cgggccgccc gcccgcgctc
    13501 gggcaacgca tcgcggtgat cggcggcggc agcgcggcga tggacgtggc gcgcagcgcg
    13561 cgccgcggcg gccacgaggt gatcgtcgta tcgctcgagg ccgaggcgca gctgccggcg
    13621 cagcgcgagg aggtgctcgc ggcgatcgac gaaggcgtgc ggctgctgca cggtgcgatg
    13681 ctgcgctcgg ccgagcgcgt cggcgagcgc ctgcgcctga actgcgtgcg cgtcaagttc
    13741 gacagcggcg aggtcgagct cgtcccgggc gccgagctct gcctgtacgc cgacgcgatc
    13801 gtcacggcga tcgggcacga cccgcagctc gcgccgctgc actggctcac gcccaaggcg
    13861 ggcgcgctga tccgcaccga cgcgctcggc gcaacgacga gcgccggcgt ctacgcgggc
    13921 ggcgacgcgg cgagcctgca gcggtacgtg acgcacgccg tcggcatggg ccggcgctcc
    13981 gcgctcgaga tcgatcgctg gctgatgggc gagctggcgc cgttgcggtc gcggcgcgcg
    14041 ccggcgagcc tcgccgccat caacaccttc taccacccgc gctcgccgcg cagcgccggc
    14101 gggcgcacgg ccgtcgccgc gcaagccgag gcgctcgccg aagccgcgcg ctgcttttcc
    14161 tgcggcacgt gcacatcctg cgacaactgc ttcgacgcct gccccgacat ggcggtgcgg
    14221 cgcgtgaacg gcggctacgc catcgacggc aactactgca agggctgcgg cctgtgcgtc
    14281 gccgaatgcc cgaccggggc gatcgtcatg cgcgaggagc tgcgatgagc cccgcgcagc
    14341 gcgcgctgct caccggcaac cacgccgtcg cctggggcgc gcgcctcgcg cggccgcagg
    14401 tgatcccggt gtacccgatc acgccgcaga cgccggtgct cgagctgctc acccagttcc
    14461 acgccgcggg cgagctcgac gccgagttcc tcacgcccga atccgagcac tcggtgatgg
    14521 cggcgtgcat cccggcgtcg ctcgccggcg cgcgcgtgtt caccgccacc gcctcgcagg
    14581 gcctgatgct gatgcacgag ctgctgcact acgcgagcgg cgcgcgcgcg cccatcgtga
    14641 tggcgaacgt gaaccgcacc gtggcctcgc cctgggcctt ctggcccgac cagaccgaca
    14701 gcctcgcgca gcgcgacacc ggctgggtgc agctgtacgt cgagtcggcg caggaagccc
    14761 tcgacacggt gctgcaggcc ttccgcgtcg ccgaagccgt gagcctgccg gtcatgctga
    14821 acctcgatgc gttctacgtc tcgcacgccc tcgagccggt gagcgtgccg ccgcaggcgc
    14881 tggtggacga ctacctgccc gcctatgcgc ccgagcacct gctcgatccc gagcgcggcg
    14941 agtcctgggg caacgtggtg acccaggaca tgtactgccg tcatcgccgg gcgatcggcg
    15001 cggcgatggc gcgcgtgccg gagctcgccg cggcggccga ccgcgaatgg cgggagcgca
    15061 ccggccgcgg ctggggcgtg ctcgagcgct accgctgcga ggacgccgag ctcgtcatcg
    15121 tcactctggg cagcatgtgc ggcacggcgc gcgaagcggt ggatgcgctg cgcgcctcgg
    15181 gcctcgccgc gggcctgctc aaggtgcgcc tgttccggcc gctgcccgcg gccgagctgc
    15241 gcgcggcgct cgccggcgtg cgcgacgtgc tggtgctcga gcgcgatttc tcgccgggcg
    15301 ccggcggcgt gctgcaccag gaactgcgtg ccgcgctcta cggcatgaag gacgcaccgg
    15361 gcgtgcacgg cgtgctggcg ggcgtgggcg gggtcaacgt gtcggcggcg atgatcgcgg
    15421 agctcgcgcg gcgcgcgcgc atcgaggacg ccgagccgga ggccgcatgg gcgtgatgac
    15481 cgacctgctg tgccccgggc acgcggcctg cccgggctgc gtcgaggcgc tgtccctgcg
    15541 gcacgtgctc gccgcgctcg gccccgacac ggtggcggtg atcccgccct cgtgcctcgc
    15601 catcatggcc gggccgcagc cgctctcggg gctgcgcatc cccgtgtacc agccggcgct
    15661 cgccaccgcc gcggcttcgg cctcgggcct gcggcgcgcg ctcgatgcgc gcggcaagaa
    15721 ggacacgctg gtgacggtgt tcgcgggcga cggcggcacc tacgacatcg gcttccaggc
    15781 cctgtcctcg gccgccgaga ggaacgaaga catcctctac gtctgcttcg acaacgaggg
    15841 ctacatgaac accggcgcgc agaagtcctc ttcgacgccg cactacgcgc gcaccggctc
    15901 gacgcccgcc ggcaaggtgt cgaagaagaa gaacctcacc gccatcatgg cggcgcacga
    15961 ggtgccgtac gcggccaccg catgcgcggg ctaccgcgcc gacctgctgc gcaaggtgga
    16021 aaaggcgaag gcgctgcgcg gttttcgcct catcacgctg ctcgtgccct gcctggacgg
    16081 ctggggcgtg cccgaggacc agggtctcgc gctgtcgcgg ctcgcggtgg aatcgggcgt
    16141 gttcccgctc tacgaggtcg aggacggcca ccgctacacg ctgaacttcc cgcacaagtc
    16201 gcgcccggtg cgcgactacc tcgagcgcca gcgccgctac cgcgagctcg acgaggccgc
    16261 cgtggcgacg ctgcagcgcg aagtcgacga gggctgggcg cggctgctcg cgcggctgta
    16321 gcgcgcctac gccgcgcgca ctgcgcgccg cgccggcggc gccgcgaaca aggccgccgc
    16381 gctgcccgcg aaggccagca ccgcgaacgt cgccagcgac gccgaatagc tcccggtcca
    16441 gtcgcgcagc gcccccgaga gcaccggccc cgaagcctgg gcgacgacct gcacggtgag
    16501 cgcgacgccg cggatcgcgc cgtagctcgc gcggccgaag taatccgccc aggcgatcgg
    16561 cagcagggtg agcaggccgc cgatgcccag gccgaacagc gccgccgcgc cgtaggcgag
    16621 cggcgcggcg tgcacggcgt tcatgagccc gctgctcgcc gccagcacga ggcccaccag
    16681 cgccagcgcg tagcgcagcg gcacgcgccg cggccaaaag ccgctggcga agccggcgag
    16741 cgcggagagc agcgcaaagg tgctgactgc ggtcgccgcc acggtcggat cgagcccgcg
    16801 ctcgatgagg aacggcgcct ggtgcaggct gatcccggcc tgcaccgggt agaccagcag
    16861 cgtgaagagc gacagcagcc agaacgccgg cgtgcgcagc gcctcggccc gcgtgaacgc
    16921 gggctcggtg gcgggcgcgg cggattcgcc cgccgccggc gcgcgctcgc cgtccgggcg
    16981 cagcccgatg tcctcgggcc ggcgcacgtg cagcacccag gtcggcacga acccgaccag
    17041 cagcacggtc gccccgaccg cgatccaggc gctgcgccag tcgctcgcct gcatcacgaa
    17101 atgcgcgatc agcggcatgg tgacgagccc ggccatcgcc gacagcgtgg cgatcgaggt
    17161 ggcgaaagcg cgccggcgca cgaaccagtt gacgatcgag ccgtagatgc cgaggtcgta
    17221 gggcccggcg aagctcatgc gcgcgacgca atacaacgtg aagaagaagg cgagcgactg
    17281 ggtgaacgag agcagcagca gcgggatgcc ggtgccgagc accgcgaggc acagcacggc
    17341 gcgcgcaccg ttgcggtcca gaaaggcgcc ggtcgccggc gccaccagcg caccgaggat
    17401 cccgcccgcc gacaccgccg ccgagatctc ggtgtgcgac cagccgaact cccgggtcat
    17461 cgggtcgatg aagatggaga gcgtcgccac cgccgtgccg gtgcgcgaga accccgccgc
    17521 gcacacgcag gcgaggatca cccagccgta gtagaacggc agccgcaagg cgagcgcctc
    17581 gaccaggcgg cggcgcagat tcatcggcgc tatattagtg ccccaaccgc ggggagcacg
    17641 tcctgaccga cataccggaa ctgcgccggc gcctgcgtgc actcgtcgag accggccgca
    17701 ccgtggcggt gcccggcgcg accgacgcgc tctcggcgcg gctcgtcgag aagcacggct
    17761 tcgaggccgt gtacatcggc agctacgcca ccgccgcgag ccgctacggc cttccggaca
    17821 cgggcctgct cacgctcgac gagctcgtcg gccaggcgcg caccctcgtc aacgcggtgg
    17881 cgatccccgt gatcgccgac gccgagggcg gcttcaacga cccggcgaac atgtggcgca
    17941 cggtgcaggc gttcgagcag gcgggggtgg cggcgatcca catcgaggac cacgaaggcc
    18001 cgggcaagca caccaagctg ccgcaaaagc tgcgcgcgcc cgaagcagcc gcggcgcgca
    18061 tccgcgcggc ggtgcaagcg cgcaaagacc cgaacttgct catcgccgca aggaccgatg
    18121 cgctctggat cggcggcgag ctcggcgacg cggtgcgccg cctcgaggcc tacgccaaag
    18181 ccggcgccga cctcgtcttt cccacgctcg ccacgccggc gcagctcgcc gagatccggc
    18241 gccgggtagg caagcccttg atgatcgtcg acctgcccga gggcacgctc gccgacgagg
    18301 aaaaagcggg cgcgtcgatc gtcctgtatt acggcctcgc cacgctggtg cagttcgacg
    18361 cgctgcagcg cgcgctcgag accttccgca ggacgcggga cgcgaacgcg gtcaccggct
    18421 accgcgagcg catcgccgag ctggaggatt tcctcggcta caaagactac gtcgagcgcg
    18481 ccaagcgctt gggcagctga tggccgccct cgacaccgcc gccgccctga tcacgctcgc
    18541 ggcgctgttc agctgggcca accaccgctt catcggcctg ccctcgacca tcgcgctgct
    18601 gcttttttcg ctcgccgcct cgctcggcgt cgtggcgctc gggcacctgg gcatccactt
    18661 cgacacgctc gcgctcgagc tgctcgccga gatcgacttc gacaaggcgc tgatgcacgg
    18721 catgctctcg ttcctgctgt tcgccggggc gctgcacgtc gatctggacg agctcgccga
    18781 gcgcgcgggg gcgatcggct ttctcgccac cgcgggcgtg ctcatctcca ccgcgctcat
    18841 cggccttgcg agctggtggc tgttcgccgc gctcggccac ccggtcccgt tcctctggtg
    18901 cctcgtgttc ggcgcgctca tttcgcccac cgacccgatc gcggtgctcg ccatcatgaa
    18961 atcggccggc accgaagcgt cgctccgcat caagatgacc ggcgaatcgc tgctgaacga
    19021 cggcgtcggc gtggtggtgt tcctcgtgct cctcggcctc gccgcgggcg gcgccgacgc
    19081 cacgcctgcg ggcgcggcga tccttttcgt gcgcgaggcg ctgggcggcg cgcttttcgg
    19141 ccttgccatc ggctgggccg cctaccggat gctcaagagc gtcgacaact accaggtcga
    19201 agtgctggtg acgctcgcgc tggtgatggg cggctacgcg ctcgccacgg cgctgcacct
    19261 ttccggcccg atcgccatcg tcgtcgccgg cctgctcatc ggcaaccacg gccggcgctt
    19321 cgccatgtcg gcgaagacca cggaaaacct ggacacgttc tgggagctga tcgacgagat
    19381 catgaacgcg gtgctgttcg tgctcatcgg cctggaagtg ctgctcatca ccttcgatac
    19441 cgagttcggc tgggcggcgg tgctcgtcat tccggtggtg ctcgccgcgc ggctggccgc
    19501 cgtctccctc gccgcgctga tgccctggct gcgccacgaa ttcccgccgc gcgtcatcgg
    19561 ccttctcacc tggggcggat tgcgcggcgg catctccatc gcgctcgcgc tgtcgctgcc
    19621 cgccgggccc gagcggggcg cgctcatcac cgtgacctac atcgttgtcg tgttctcgat
    19681 cctggtgcag gggctgacgc tgtcgcggct cctgcgccgc gcgagccccg ccccttcaca
    19741 ctagacccag ggagcaaacc atggcgaaat cacccaagcg cagcaaggcc tataccgagg
    19801 gactgaagat ccgccgccgc tggctcggca gcgcgtacgt cgacaaggcg ttcaaggacg
    19861 ccgacgactt caccatcgac ctgcagcact acgtcaccga gcacggctgg ggcgcaagct
    19921 gggcgcgcgg cgtgctgccg ctgaagacgc gctcgatcat gaacctcgcc atgatctcgg
    19981 cgctgaaccg cccgcacgag ctcgagatcc acctgcgcgc ggcgctccgg aacggcatca
    20041 ccaagcgcga gatcaaggag atcttcattc atgtggccac ctactgcggc gcgccggcgg
    20101 cgctcgacag cttccgcatc gcgaagaaga tcctcgccga gaagccggcg cgcgcatgac
    20161 gcagctcagg atcggcgcgg cggtcgcggc gcgcatcgag gagagctacg agccgaactt
    20221 cagggcgtcg aagttcttcc ccgactggga cgaggcggtg gcgaaggagc acgccgcctg
    20281 gctcgcgccg gatcactacg acccgggctc ggggctgctg aagctcagcg tgcacagctg
    20341 gcttctcacg atcgacggca agcgcatcct gatcgacgcc tgcgtcggca accacaagcc
    20401 gcgcgagaag cgcccgttct ggcacatgat gaacacgccg tacctggagc gcctggcggc
    20461 ggccggcgcg aagcccgagc agatcgacat ggtgatgtgc acgcacctgc acgtcgacca
    20521 catcggctgg aacacgcgcc tggagaacgg gcgctgggtg ccgacctttc ccaacgcgcg
    20581 ctacgtctgg agccgcgagg acttcgagca ctacctcgcc atcgaccgcg acgcggagaa
    20641 gggccccgcc aacctcgggt cgttccgcga ctcagtcctg ccggtggtcg aagccgggct
    20701 cgcgcagatg gtctcgggcg ccgcggcgct cgatgaaagc ctctccgtgg cgccggcgcc
    20761 cgggcacacg ccggggacga tcgccatcaa gttcgagtcg cgcgggcaga aggccttgtt
    20821 ctgcggcgac atcctgcacc acgcgctgca ggtctaccac cccgagtgga acagcttcgc
    20881 ctgcgccgac gcgcacaacg cgcgcgcgag ccgcaggaag gcgctcgagg attgcgccgc
    20941 cggcgcgctg ctgatgccgg cgcacttcgg cgcgcccttc gtgtgccgga tcgaggcaaa
    21001 gggcggcggc ttccgcccgc gcttctagcg ccgcggcccc gcgggcggct cgcagcccgc
    21061 ggcggcgccc aggcccttca ggaacgagcg gcgcacgacg cccgcggccg ccgcggcggc
    21121 gacctgccgg gaatggcgct cggcgccgct cagcctgcgc acccagctgc gcatcggcac
    21181 cacgccctcg gcggcgctgc gcaccgtgcc gaccgcggcg tcgccgaccg tgtcgatgcc
    21241 ccgctcgatg acgctcggct tcgggccgct gtcggccgcc gcgtcgaggt cggcgccgag
    21301 ctcggcgtcg agcaacgcca cctcacccgc cagcccggcg cagctgccgt cggcgggcgg
    21361 cgcgtagggc gcatccagcg ccgaaacgag tattggcggg atcttcgcgc gcagcaggtt
    21421 gaggtcggtg aggggcgccg tgaccgcccg ccccatcggg tcggtcgggg cctcctgcgg
    21481 cgtggcgcag gcggccagcg cgcaggccag cccggcgatc gcgaaatgac gcatcggtct
    21541 tcctccgccg cgccctcgcc ggacttgccg catccggggc gcgcgcgcac attgtatgca
    21601 tgcgccacct ggagaccggc atgaagacct ggacacgacg tgaattcgcc accggcgcgg
    21661 cggcgctcgg cgccgccggg ctgctgcccg cgcgcgcggt ggcgcaggat ctgctcaccc
    21721 gcgcgatccc gaagagcggc gagcgcctgc cggcggtggg cctgggcact tcgcgcgtgt
    21781 tcaacgtcgg cgacgacgcc gaaaagcgcg ccgcgctggc cgaggtgctg cgcaacctgg
    21841 tcgcgggcgg cggcaagatc gtcgacaccg ccacgtccta cggctcctcg gaagccgtgg
    21901 tcggcgacct cgtggcgcgg gcggggctgc gcgcgcaggt gttcatcgcc accaagctgc
    21961 gcgcgagcga cgcggccgag ctgcgcggct cgctcgagcg gctgcgcacg caacaggtgg
    22021 acctgctctt tctgcacaac gtgcgcgacc cggcgcagga cctggggcag ttccgcgact
    22081 ggaaggccgc gcgcgcctgc cgctattacg gcgtcacctc gacctacgaa ggcgactacc
    22141 ccgcggtcga agcggtgctc aagcgcgaga agcccgattt catcgagatc gactactcgg
    22201 tggacaaccg cgaggtcgag cagcggctgc tgccgctcgc tgccgagacg ggggcggcgg
    22261 tgctgatcgc cctgcccttc ggccgcgcgc ggctgtttcg cgcggtgcgc ggcaagagcc
    22321 tgcccgagtg ggcgcgcgcg ttcggcgcgg cgagctgggc gcagttcttc ctcaagttcg
    22381 tcctcgcgca cccggcggtg accgccgcca tcccgggcac ctccgacccc gcgcacatga
    22441 ccgataatct gggcgccttg cgcgggcgcc tgcccgacgc cgccgagcgc aagcgcatgg
    22501 tggcattcat cgaatcgctc tgaaggaggt cctcgtggaa cgccgcggtt ttctccagtt
    22561 tgccgcgcta ctcgcggcgc tgccgctcgc cgcgcgcgcc gcccagccgc tgaagatcgg
    22621 caccctcggc gcgggccgca tcggcagcac gctcggcgag ctgtgggtga aggccggcca
    22681 cccggtgatg ttctcctcgc gccacccgga ggagctgaag ggcctggtga ccagcctcgg
    22741 tccgctcgcg caggcgggca cggtggccga cgcgatcgcg tttgccgacg tggtgctgct
    22801 cgccgtgccc tatcgcgcgc tgcccgagat cggaaaggcg catgcgggcg cgctcgcgca
    22861 aaaggccgcg gtgatcgacg cctgcaaccc gtttccgcat cgcgacggcg agatcgccgt
    22921 ctgggcgcgc gagaagggcg ccggcctcgc gacgcaggcg ctgctgcccg gcgcgaagct
    22981 ggtgcgcgcg ttcaacgcca tcagctacgc gcgcatggcg cagatcgggc gcggccgcga
    23041 gggcgtcggc atgccgatcg cgggcgagga cgcgggcgcg ctcgaggtcg ccgcgacgct
    23101 gatccgcgaa gcgggcttcg agccggtgcg agtgggcggc ctcgccatgg gccggcacct
    23161 cgtgccgggc acggcgctcg cgggcgagca cagcccggcg gagatccggc ggatcgcggc
    23221 gacgctgcag tagaaatgaa aggccccgca cgaggcgggg cctgtggccc gagtctacgg
    23281 gcagcgcttt gtaggcgctc ttgcgatgcg cggacgtgcc gcgcgctgga ccgcttgccg
    23341 aggggcgctc tcccgactgg tcgccgggta tcctgctggt gtcgccggat cgctttcgcg
    23401 ccggcttgcc ctcgttggtc cgcttccaac atagcaccgt ggcgcgcggc gtcaatggga
    23461 gaaatggatt cgcttaacgc agatcaaaca ttcgaaccgc gccgggcgaa gggttgaatt
    23521 gcggcgcgtt tgccctcact gtccgcaaat ggacatcacc acgcagactt tcgaagccga
    23581 ggtcctcgag gcctcgcgga cgctccccgt cgtcgccgat ttctgggcgc cgtggtgcgg
    23641 gccgtgccgc gcgctcaccc cgaccctgga aaaggtcgcc gccgactacg cgggccaggt
    23701 gaagctggtg aagatcaact ccgacgacaa ccaggagctc gccgcggcgc tcaacatccg
    23761 ctcgatcccg aacgtgatcg cgttcaagga cggcaagccc gtggcgcagt tcatgggcgc
    23821 ggtgccggag tcgcaggtgc gcgcgttctt cgacaagctc ctgccctccc cgtccgagct
    23881 cgcgctcgcc gcggccgaag cgctgtacca ggcgggcaag ctcgacgcgg ccgaggcgaa
    23941 gctcgccgag gtgaagttcg accccgactg ggacgacaag gtcgaggcgc tgaagcaggg
    24001 catcgcctac gcgcgccagg gcgagggcgg cccgggcgaa aaggagctga aggcgcggct
    24061 cgtcaaagac cccgcggacc acgacacgcg cgcggcgctc gccgcgctct acgccggcag
    24121 caagcgctac ggcgaggcga tggacgagct gctggagatc acgcgcaagg acaaggactg
    24181 gaaggacggc gacgccaagc gccagctgct cgcgctcttc agcctcgccg cggaccagcc
    24241 cgagctggtc tccgcgtacc gcaaaaagct tgcgcaatca ctgtactagg cttgcgccgg
    24301 cacccccgcc tgggggataa tgccggcctg ctgaggagcg ctgcgtcccc cgccattcaa
    24361 gggagtcagg ctcagcaaaa tcaacggcgc tcgcatcgaa accggtgcgg gcgccttgtc
    24421 gtttcagggg cccgccgaac gtgaaaggag ctccgcaatg aacgcaccca acccgaagtt
    24481 caccgactac aaggtcgcgg acctgtcgct cgccgactgg ggccgcaagg aaatcgccat
    24541 cgccgagtcc gagatgccgg cgctgatggc gatccgcgag gagtacgcga agcagcagcc
    24601 gctgaagggc gcgcgcatca ccggcagcct gcacatgacg atccagaccg cggtgctgat
    24661 cgacacgctg aaggcgctcg gggccgaggt gcgctgggcc tcgtgcaaca tcttctccac
    24721 ccaggaccac gccgcggcgg cggtggcggc gagcggcacg ccggtcttcg cctacaaggg
    24781 cgagtcgctc gacgagtact gggacttcac ccaccgcatc ttcgagttcg gcgcccaggg
    24841 gccgaacatg atcctggatg acggcggcga cgcgacgctg ctcgtgcacc tcgggcaaaa
    24901 ggcggagaag gaccccgcgg tgctcgcgaa gccggcgagc gaggaggaaa cgtcgctttt
    24961 caaggcgatc cagaagaagc tcgccaagga gcccggctgg tacacccggg tggcgaagga
    25021 gatcaagggc gtcaccgagg agaccaccac gggcgtgcac cggctctacg agatggcgaa
    25081 ggaaaagcgc ctgctctttc ccgcgatcaa cgtcaacgac tcggtgacga agtccaagtt
    25141 cgacaacctg tacggctgcc gcgagtcgct ggtggacggc atcaagcgcg ccaccgacgt
    25201 gatgatcgcg ggcaaggtgg cggtggtcgc cggctacggc gacgtcggca agggctcggc
    25261 gcaggcgctg cgcgcgctct cggcgcaggt gtgggtgacc gagatcgatc ccatctgcgc
    25321 gctgcaggcc tcgatggaag gctaccgcgt ggtgaccatg gactgggccg cggacaaggc
    25381 cgacatcttc gtcaccgcga cgggcaacta ccacgtcatc acgcacgagc acatggcgaa
    25441 gatgaaggac caggccatcg tctgcaacat cggccacttc gacaacgaga tcgacgtcgc
    25501 cgcgctcgat aagcacacct gggaggaggt gaagccgcag gtcgaccacg tcatcttccc
    25561 cgacggcaag cgcatcatcc tccttgccaa ggggcgcctg gtgaacctgg gctgcgccac
    25621 ggggcacccg agctacgtga tgagctcgtc cttcgccaac cagaccatcg cgcagatcga
    25681 gctgttcacc cacaccgcca agtacccggt gggcgtgtac acgctgccca agcacctcga
    25741 cgagaaggtg gcgcgcctgc agctgaagaa gctcaacgcg cagctcaccg agctgaccga
    25801 gaagcaggcg gcctacatcg gcgtgccgaa gcacgggccg tacaagtccg accactaccg
    25861 ctactaagac gtgcgacgcg cagtcctgct tgcctcgctc ctcgtcgtct ggctgatcaa
    25921 cgccgtcgcg ctgctcacgg tggcgtacgt cgtgccgggg atcacggtgg cggacttcgc
    25981 cgccgcgctg gtcgcggcgg tggtgctggc gctcgtcaac accatcatcc gcccgatcct
    26041 gatcctgctc acgctgccgg cgacgctggt cacgctcggt cttttcatct tcgtcatcaa
    26101 cgggctgctg ttctggctgg tcggcagcta tctcgagggc ttcgaggtgg cggggttctg
    26161 gtcgggcgtg ctcggggcga tcgtgtacag catcgtctcg tgggcgctcg ccgcggtgct
    26221 gctgcccgag cgccgcgtca gagtcaccgt gcacagggtc gaccggccat gagaatcgcc
    26281 gtcatgggcg cgggcggcgt cggcggctac ttcggcgcgc gcctcgcgca ggccgggcac
    26341 gaagtgtcgt tcgtcgcgcg cggcaagcac ctcgaggcaa tcagggcgaa gggcctcagc
    26401 ctgaagagcc cgctcggcga cgcgacgctc aaggtgaagg cctccgacag gcccgccgag
    26461 ctgggcgacg ccgaaatcgt gctgttcgcc gtgaagctct gggacaccga gagcgccgcc
    26521 gaggccatcc gcccgctggt ggagaagggc ggcgtggtga tcccgttcca gaacggcgtc
    26581 gagtcgatcg agcgcatcgc caaggtgctc ggcgccgagc gcgtgatggg cggcgccgcc
    26641 tacatcgcgg gccgcattgg cgagcccggc gtcatcgtgc agaccggcac catggcgcgc
    26701 cttcgcttcg gaccggtgct gccggcgcag aagaaaaccg ccgaagcgtt cctcgctgcc
    26761 tgcaagggcg cgaagatcga cgccgagctc accgacgaca tcgtgagagt gctgtgggag
    26821 aagttcgtgc tgctggtggc ggtttcggcc acgaccaccg tgacccgcgg caacctcggc
    26881 gtggtgcgcg cggatcccga cctgaagtgg ctgctcgagg cctgcatgcg cgagacctgg
    26941 gcactcggca ggaagaaggg cgtgaagctc gccgacgacc tcgtcgagca gacgctgaag
    27001 atggtggacg gcctgcccgc ggaaatgcgc gcctcgatgg ccgcggacct cgaggcgggc
    27061 ggcaagctcg aagcgccctg gctctcgggc gcggtggcgc gcatgtcgcg cgaggcgggg
    27121 ctcgaggcgc cggccaaccg cacggtgttc gcggcgctca aaccctgcgt cgaaggcagg
    27181 gcctgatggc ggccggcgag cgcgtcttct cgttcgagtt cttcccgccg cgcacgccgg
    27241 aaggaaaagc gaagctgcgc gccacctggg aggcgctcgc gcgcctcaat ccgcgctttt
    27301 tctcctgcac cttcggcgcg ggcggctcga cgcgcgcggg cacgctcgag acggtgctcg
    27361 agatccgcaa ggccggacac gaggctgcgc cgcacatttc ctgcgtcgcc tccacgcggc
    27421 aggacatcgc cgagcagctc gagcgctaca aggcgcacgg catccgccac atcgtcgccc
    27481 tgcgcggcga cctgccttcg ggcatcgccg cctcgggcga attccgctac gccagcgagc
    27541 tcgtcgcctt cgtccgcgag tccacggggg actggttccg catcgaggtc gcctgctacc
    27601 ccgagtacca cccgcagacg cgcaatgccg ccgacgagat cgtcaatttc aagaagaagg
    27661 tcgacgccgg cgcgaatgcc gcgatcacgc agtacttcta caacgccgac gcctatttcc
    27721 gcttcctcga ggagtgccgc gcggcgggca tcggcgtgcc gatcgtgccg ggcatcatgc
    27781 cgatcggcaa tttctcgcag ctggcgcgct tctcggacgc ctgcggcgcc gagatcccgc
    27841 gctggatgcg cgtgaagctc gagggctatc gcgacgacag cgcctcgatc cgtgccttcg
    27901 gcctggacgt ggtgagcgcg ctttgcgaga agctgctcgc cgccggcgcc ccggggctgc
    27961 acttctatac cctcaaccag gcggcgctca gcaccgagat ctggcggcgc ctgaagctgt
    28021 gaagcggctg ttcgcggcgt tcgccgccgc cgcgctcgcg ctcgccgcgc tcgaagtctt
    28081 cctcgctcac ggcctgcagc cgctcgagca ccgcctgctc gacgccttcg tgcgctggca
    28141 tgccgcgcgc ctcgcacccg acccggcgat cgtcatcatc gacatcgacg aaaagagcct
    28201 ggcgcagatg caggacgcgg cggggcgctt tccctggccg cgcgtggtgt acgcggagct
    28261 gctcgaaggc ctgatggcgc agaagccgcg cgccgtggtg ttcgacctcc tgttctcgga
    28321 gaaggaccgg ttccgccccg aaagcgaccg cgccttcatc gaggcggcgc tgccccaccg
    28381 caacgtcttc ttccccatgc tgcggctcga cccgcgcgac gatgcgaacg gcgcgagcgc
    28441 cgcggagctc gcgccgctcg tgggcctggt gcgccgaccc gacgccgacc cgcagacgcg
    28501 catcgccgtg gtgccgccgc tcgcgctgcc tgccgagctc tggcgcggcg ggccgatcaa
    28561 cttcctcgag gacgccgacg gcgtcggccg gcgctacctg ctgcgcacgg tggtcggcgg
    28621 ctgggcgctg ccgtcgctgc ccgcgcgcgt ggcgctcgac ctgggattcg gcgtgcccga
    28681 cgccgacgac ctgatcctcg cctggcgcgg cacgccgcgc gggctgccgc gcgcgtcgtt
    28741 cgccgacgtg taccaggact tcgggcgcgc ccggcgcgag cgcccggcgg acgagttcac
    28801 gggcaagatc gtcgtcatcg gcgccgcggc gcccgggctg ggggacctgc gcgtgacgcc
    28861 gctctcggcg acgcagcccg gggtcgagat gctcgccacc gccatcgaga acctgcagaa
    28921 cggccgcgcc atgcagcgcg cgccgcagtg gctgccgttc ggcacggcgc tcctgctcat
    28981 cggcgcgctc ttcgccgcct ttgcgcgcaa cgtcgacgcg agccgcagcg cgctcgccct
    29041 gggcgcggcg agcgcgctcg ccctcggcgc gagctggctc gccgccggga cgctgctgct
    29101 cgtgccgctg ctcgcgccgc tcgcggcggc gtggctgtac tacttcgccg cggcgctcgc
    29161 cgcctacctc agggagcgcc gcgcgcgcca ggaaaccgtc gccatgttct cgcgcttcgt
    29221 gaatccccac gtggtgcgcg cgctgctgga gcgcggcggc ctggaaggcg cgggccagac
    29281 gcgcgaagtg acgctgcttt tttccgacat ccgcggcttc accacgctct ccgagacgcg
    29341 agcgcccgag gaggtggtgg cgatcctcaa ccgctacttc acgcgccagg tcgaggcggt
    29401 cttccgccac ggcggctcgc tggacaaatt catcggcgac gccatcatgg cattctgggg
    29461 cgcgccgctc gacgacccgg agcacgcgcg gcacgcggtg gcctgcgcgc tcgacatggc
    29521 cgacgagctc gccgcgttcc gcgaggagct gggcgaggcg ggcgcggggt tcgacgtcgg
    29581 catcggcctg cattcgggcc cggcggtggt aggcctgatc ggctcggata agcgtcgcga
    29641 gtacacttcc atcggtgaca cggtgaacct cgccagccgc atcgagggcc tgaccaagga
    29701 tgccgggcgc cgcattctcg tttcgcgcca gaccatggaa cggtgccaag gcgccttcga
    29761 tttcgtatca tgtggcacct tcccggtgaa aggccgcgcc cagcccgtgg agctgttcga
    29821 gcctcgcaga agaccatgaa agccgtcctg ctcgcgctcc tgtttccccc gctcgcgctc
    29881 gccgcgcccg ctaccgtgat ccgcgcggtg gacctcaagc aagcgcccgc cagcgacgcc
    29941 gccaccgtgg ccgcgctcgc cgagcagtcc ggggtcgagg tgctcgagcg caagggcggc
    30001 tggacgcgcg tgagaaccgc ggccggcgcc gagggctggc tgcgcatgct cgcgctgcgc
    30061 tacgcggcaa cgggcgacgc gaagcagggc gacagcggca tcgtgcagct cctgaacgtc
    30121 gcgcgcaccg gcacgagcgg cacgcaggtc gccacgggcg tgcgcgggct ggaccccgag
    30181 cagctcagga acgccaagcc caatgccgcg gagctcaagc gcctgcagga ctaccgcgcg
    30241 agcccggacg cggcggccgc gttcgccgag cgcggccggc tccagcccaa gcaggtggcg
    30301 tatccgaagg agggcggatg aggcacgccg cgacgctgct cgccgcgctc gcgctcgccg
    30361 cgcccgcgca ggcgcagttc aacatcaacc tcaaccggct gctcgacgcg ggcaagaacg
    30421 tgcgccaggc cgcgggcgag atcgacgaga aggaggagat cgagctcggc ggcgacgtgg
    30481 ccgcgcggct gctgggcgcc gcgccgctgg tgccgaacgc ggcgctgcag acctacgtga
    30541 accgcgtcgg gcgctggctc gcctcgcaga ccgagcgccc gaacctgccg tggcagttcg
    30601 gcgtgctcga tgccccgcag gtgaacgcct tcgccacgcc cggcgggcac atcttcgtca
    30661 cgcgcggcct gctggcgcgc atgaagaacg aggccgagct cgcgggcgtg ctcgcccacg
    30721 agatcgcgca cgtgctgaag aagcaccacc tcaaggcgat ccagaagaac gcgcagctcg
    30781 agctcgccgg caccgcgctg agcttgtcgc gccgcaccga ggacgccgac aagcgcgcca
    30841 agctcctcgc cgccggcagc gagctctatc tgcgcggcct ggacaagtcc gacgagctcg
    30901 aggccgaccg cctgggcgtg gtgatcgccg cgcgcggcgg ctacgacgcc tacgggctgc
    30961 cggcggtgct gcagacgctg caggccatga accccgagga ctcggggctc gcgctcatgt
    31021 tcaagaccca tcccgcgccg gccgagcggc tcgacgcgct ggaacgattg cagcccgtgc
    31081 tggacgccca cgccgcgcag ccgcagctcg cggagcgctt cgtgggcgcc gtctccgggc
    31141 gttgaaagga gcctctcatg aaaaaattcc tgctgctcgc aacctggctc gcctgcggtc
    31201 ccgcgctcgc gcagcacggc accggctggt acatcggcgg cggcgtcggc acgacgaagg
    31261 ccgatttcgt gcgcagcgac ttcaccggca ccggactcgc caccggtacg tactcggccg
    31321 atgacgacga cgtcggctcg cgcttcttcg gcggctaccg cctggcgccc aatctcgcga
    31381 tcgagggcgg cctcgccttc ctcggcagct acaagcaccg cttcaacaac ggcggcaacg
    31441 tcgcggtgta cgactacagc gcgagcgcgc tgaccttcgc cctcgccggc aacgtcccgg
    31501 tggcgggcgg gttgtcgctg aacgggcgca tcggcatcgc cttcgccggc agtgagctgc
    31561 gcctgcgccg caacaacggc accgccatag tccccttctg cccggatagc tggtggtaca
    31621 ccgactgcgc gtcgcagtcg acgaacctct actggggcgt gggcgcgcag ttcgacgtca
    31681 gccgcaactg gggcatccgg ctcgattacg acaactacgg cgaggtgggc gaggagttcg
    31741 agaccggccg cgccgacatc gagcaggtgt cggtgaactt cgtctggcgc ttctagtagt
    31801 tctcgcgcag gaagatgaag ttcttcggct gcgagccgaa gaggccgaag ccggcggacg
    31861 cgctcggctt gtcgtcgtag gccgcaccgt cgtcccactt gccgagcagg taggccttgc
    31921 ccgcgtcgct cgccgggagc tcgctccccg gcgcaccgat cgccgggcaa tggtctcctc
    31981 cggccgagcc caggttcgcg ctcagccgca cggtaccctc gttgccggcg ccggggctgg
    32041 ccatggtgat cgttgcccgt cccgaggcca gcgtgaggtt tgcctcctgc atcgccgtct
    32101 cgcaggcgac caggttcgcc ccgcccgcga aggcgagcgc gaaatctgag cgcacgaacg
    32161 gcgtgcagct gtcggcggtg ttgcgcgtga agccgggcgt gggcgaagtc ccgtcccagt
    32221 actgcgcttc cagcgtcacg ggaaggtcgc ggttgctcgg gccatacagg ctctgcagca
    32281 cgagtcggcc gaagcgcact gcggtggcgg gtccgacctg ggcgcgctcc ggcgtcgcgt
    32341 cgttgtcggc gtcgaggttg agcgccgcgc cgagcagcgt gacgctgtcg gggtcgctcg
    32401 gcgcgatgcc gatgcgcaac gtgccgtagg gaccgtccgg cccggcggcg gcgcgtgaca
    32461 ccgcgatcgg cgcggtgatg gcgcccgtcg ttcccgccgc ccaggtcgcg gcgatgccgg
    32521 ggatcatccc caggtcgagc cgcgccgtga gcggcgtggg cgcggcggcg tcgatggcgc
    32581 cgaagttgta cgacgccgcg ctgttcagca ccagggcgcc gagcgtggcc cccgagtagc
    32641 ccggcgtgac cgcgccgccg gcttcgcgcg cggtcagcgt aaagcccagg ttcatacgct
    32701 cgtccatgta ggtgaagctc gagcccgcgc aggcgccgat gtccgtgcgc ggcgcgagcg
    32761 aggtcgccga cagcgtgaag tgatcgggca cgaaacgccc ggcggtgacc ggcgccgagc
    32821 tgatgtagcg caccgaggtc gccgtgccgt cgccgttgtc cacgctggag aacgtctggt
    32881 cctggaggac gaggctgaat gcgcccacat cgccgtagct cgcggtgctg gtcgtgatca
    32941 cgccggcggc tgccgaccag gcgccgggcg tgagcgtgcc gagcgccgcg gggcagacgg
    33001 cgcccgcgcc gcactgcgac aggatcacca ctggttgccc cgccgccggg ctgtagagcg
    33061 tggtggtcgc cggcgtcccg gcgccgtttt gcgccgtcgc gtcgatgcgg aacggccgcc
    33121 cggcgttgtg caccgtgccg gtgccgggat tggacgtgtt gttgagcgtg cgcgtcgtgc
    33181 ccgcggtggt gcggttcgcg tcgcgcgcca gcacgctcac gaactgcagc ggccggttgg
    33241 caaacgcgtc ggacgagcaa ctggtcacgg taggcgcggc cgtcgggtac ttgacgcgga
    33301 acagcacgtt acgccacgcg ttggcctcga attgcccggc gctcagcagg tgccggccgg
    33361 cgtcaccgac ggtgaaggtc tggttggcca gcgccttgat gagcggcagg ccggcgcacg
    33421 cgccaccgct cgtgttgtcg accagctcca ccgcgacgac gccggtgaag ctggtcgcga
    33481 tgttgttcga ggcatcgcgc gcgacgatgt cgacggcgat gtcctgcccc gcgatcttgg
    33541 tgaagatgcg cccggtcacg gcatcgccgc ccggctcgac cacgttgaag ctcgacaccg
    33601 agacgccgac agtgaggatg gcgaaggtcg gcccgccatt gccggcgttg gtggtggaaa
    33661 cgacacccgt gccgttgttg tagctcgccg ctaccgatga ggtgacgctg acgctcaccg
    33721 cgcagctgct cgaagccggg atcgttccgc cgttcaacgt cagctgcgtg ccgccggctg
    33781 cggcgctcgc ggcgccgccg cagctattcg tgaggcccgg cacggcggcg ttgacgaggt
    33841 tcgcgggata gctgtcggtg aaggcgacac ccgtgatggc gacggtgttg gtgttcgtga
    33901 gggtgatggt cagcgtgctg gtctggcccg tgccgatcga ggcggggctg aagctcttcg
    33961 ccacgccggg cgcgccgttc accgtcagcg tcgccgaggc ggcggtgccg ctgccggcgt
    34021 tggtggaggt caccgtaccg gtgctgttgt tgaagctgcc tgctgagggc gaattgacgc
    34081 ttacgctcac cgcgcaactg cccgaggccg gcaccgtgcc gccgctgagc gtgagctggc
    34141 cgacgcccgc gttgccggtg gcggtgccgc cgcaggtgtt acttagcgca ggcgtcgagt
    34201 gattgaccag cgcgcccgga taggtgtcgg tgaacgccgc gccggtgatg gcgacggtgt
    34261 tcgaattgct gatcgtcacc gtcagcgtgc tcggcaggcc aacgccgacc gaggccgggc
    34321 tgaagctctt ggtgacagtg gggcgattgt tgatccgcag cgtggccgcg ctggcggcgc
    34381 cattcgcagc attggtagag gtcaccgtcc cgctgctgtt gttgaacagg cccgcggtcg
    34441 ccgaggtcac gctcacgctc actgtgcagc tcgcgccggc ggcgagtgtg ccgccggtga
    34501 gcgtgagctg attggcgccc gcgttgccgg tcgcggtgcc accgcaggtg ttgctgaggc
    34561 ccggcgtcgc tgcgttgacc agcgtgccgg ggtaggtgtc ggtgaacgca agcccgacga
    34621 tcgagcctgc ggcgttgggg ttggtaagcg tgatcgacag cgtgctggag agccccgtgc
    34681 cggtggcggt cgggctgaag cccttcgtga cgttcggcgc ggtgagctgc gtgaaggtca
    34741 cggccgtggt ctgcgtgatg acgacgctgt cggtggtgac ggtcgccgtg tacgtgtcgg
    34801 tctgcggcgc ggtgctcctc acggtgaacg tcgcgacgcc gctggcgttc gtgaccgcgc
    34861 tcgccggcgt gatggtcgag ctgccggttt gcgcgagggt gacggtcttg ccggagagcg
    34921 gcgtgccgca cggggtgcgc accgtcaccg tgatcgtggc ggagctgctg ccgtccgccg
    34981 gcacgctggc gggcgcggcg gtcacgctcg actgcgacgc gctggcgggg gagttcccgg
    35041 tcaccgtcaa cgttccagag ctcgcggtta cggtgcccgc ggtcgaggtg accgggaatc
    35101 ccccgaccgg atagctgccg gcgtcgcagg aaaagaccgt cacggtgacc gtgcagctgt
    35161 tgttgccggc gatgcgcgcc ccgctcaggc tcaccgagct gccgagcggc gccgccgaca
    35221 cgatgccgtt gctgcagtcc gtggcgacgc tcggcgtcgt gtgattgaag aggttggccg
    35281 ggtagttggc ggtgaaggag atgttgtccg ggttgcccgg cggactgttg gcgagcgtga
    35341 tggtcagcgt cgaggtgccg cccccgccga tcgaggcggg gctgaagctc accgtcgcgc
    35401 cggtcaggtc cgccaaaacc gagggcgcgg ccagcagcga aagtacgaca acaggaattc
    35461 gcaggaagcg catggacgct cggagaacga ccctaactta gcaccgagcg gcacatcgct
    35521 ggcgtgaagg attcccccct caatcgaccg cccgacgaga ggtatccccg gtgcgctcga
    35581 aggcgcgcca gggaccgccc ggccgttcct ggcaagcgcc gtaacggaac ggcagccagt
    35641 cgcgcccggg gttcaaaaat cgcaagtcca cgaaccaggc gcaggtcgag cctgctgtca
    35701 gcccgtcgaa ggccggcttg tcggcgaacc agcgcatgaa gccgagcgcc ggggaggccc
    35761 atgcctcgcg cgcgatgcgc tgctcggctt cggtcgcgcc gtagcgcgtg cgcgtctccc
    35821 agcgcgcgtg cgcgagcggc tgaaacgcgg cgttcagtcg cccgacgaag tcgtgcgccg
    35881 ggggcaaggg ctcggtgcgc cgcaggttca cgtgcgcgaa acggtgcgcc gcctcatcgc
    35941 tcgcgaacac ggtccagttg aagggcgtca ccgcacccgg gtgcgcggcg agctcgacgc
    36001 cgctccagcc ctgcttcgcg gcgtactcgc gcgcgtagtc gagggcctgc tccttgagca
    36061 gcgcctgaaa acccacgtag gcgacgagca ccgctcccgc cgccatcgcc ggaacgcgcg
    36121 agcggcgcca ggcaagcgac gccagcagcc cggcgacgat gatgccggtg aaccacaggt
    36181 cgatgatgaa cgtcgcgccc caggcgtagc gctcgtagga gaacggcgaa aagaccagcg
    36241 tgccgaagct cgtgatcagg tcgccgacga tgtgcgcgcc gagcgcgagc gcggtaacgc
    36301 cgtagagcgc gcgccagccg cgcggctcgc gcagcagctt cgccagcacc caggcaagca
    36361 gcagcgccca cagcggcagc aagatgaccg agtgcgtagg gccgcggtgc tgctcgagat
    36421 agaacaccgg cgagacgaag gcgaggacga agtcgagatc cggcgcggcg caggcgaaga
    36481 agcccgcggc cacgcgccgc ggcagcgagc gcggcggcgc gtccttcggc gccgtcgccc
    36541 gggcgagcag cgcgcccgat aacgcgtggg tcagcgtgtc cataaatggg gacagacctc
    36601 atttatctga caaacaagat aaatgaggtc tgtccccatt ttagctacgc ggcgcgcttc
    36661 ttcctttgcg cgtccttggc gtgcatctgg gcgtaggcgc cgtgcgcggc ggattcggcg
    36721 tcgccgacgt gcaccgggta gcccatgtcg tcgagcaccg agccgagcgc cgacaggcac
    36781 agcatcacgt tgtccgggcg gcaggagtag cccatcaggc cgaagcgcca gatcttgcct
    36841 ttcaggggcc ccaggcccgc gccgatctcc aggccgaact cggagagcag gcgcgagcgc
    36901 acctgcgcct cgtcgacgcc gtccggacac agcaccgcgt tcatctgcgg cagctggtat
    36961 tcctccttga cgaggaactt gagccccatc gcttccaggc ccgccttcag cgcgacgtgg
    37021 tggcgctggt ggcgcgccca gcaattgtcg agcccctcct cgcgaatcag cagcagcgcc
    37081 tcgtgcagcg caaagagcga gttggtcggc gcggtgtggt ggtaggtgcg cgtcgtctcg
    37141 ccccagtagc cgagcagcag gttcatgtcc atgaaccagc tgtggatctt gtccttgcgc
    37201 ttcttcacgt gctcgacgac acgctcggag aacgacaccg gcgacagccc cggggtgcac
    37261 gacaggcact tctggctcgc cgagtagatg gcgtcgatct cccactcgtc gaccttcacc
    37321 ggcgtgccgg caagcgacgt caccgcgtcg acgatgacga gcgcgccgtg cttgtgcgcg
    37381 atcttcgtca gctctttcgc gtcggactgc gcgccggtgg aggtctcggc gtggacgaac
    37441 gcgacgacgc tcgcgccctt cgcctttttc agcgcatcct cgaccttttg cgggtcgacg
    37501 ggctcgcccc acttgtcctc gaccacgatc ggcgtgccgc cgcagcgcag gacgttctcg
    37561 agcatgcggc cgccgaacac cccgttctgg cacaccacca ccttggcgcc cggcgccacc
    37621 atgttgacga agcagaactc catgccgacc gagcccggtc cggagagggg gaacgtgagc
    37681 gggtgcttcg tctggtacac gtagcgcagg agcgatttca gctcttccat catctcgacg
    37741 aagatgggat cgaggtagcc gatcgccggc tggctcatgg tggtgagcac gcgcggatgg
    37801 atctcggtgg gcccggggcc catgagggtg cgctgcggcg gatgaaagga cgtgatgcgc
    37861 ttcgaggggg cgtactgggt catggcttgg ctctccgcgg tgatgccgaa gggcgcatcg
    37921 tccgggcgcg atttcacgcg caccgggttc ttggcgctcg cttcattgag gatctccacc
    37981 gcgctgatgc ggcccccggt gacgcgctcg acctcgccgg cccagcgcac cggcacgtag
    38041 ccggtcttca cccagttgtg cacgaccgcg cggtcgagct gaaatgcgcg cgccacctcg
    38101 gcctggctgc cgaaaatccc gaccagtcgc tccgcgcagt tcatggtggc gcggagttta
    38161 ggacgtcaat tctattttga caagcgtagt ttcgggcggc cggagggccg atcgggcccg
    38221 gcctcgaggc gcgccaggcg ccgctcgtgc tcgaggatga acttctggtg gtcgcgcagc
    38281 gtggcgtgca ggtcgttcag ccggtcgcgc agcgtcgaga cgcccgagag ccgcccgagc
    38341 atctcctgga tcacgctcat cgcgcctcgc ccttgcgcag ccggcgcatg tcgcgcagga
    38401 acgccgcgtg gcttgcgacg atctggtcca cggtcttcac catgcccgcg atctcggcct
    38461 gcgcgtggcg cgttgcggcg tcgagcagct cgagctcctg cggcgtcacc cccgcaaggt
    38521 aggcgtcgac cgcggagcgg atctcggccg ccattttcgt gttgcgctgc tgcgcgcgcc
    38581 gcgcgaggcc cgtcttttgc tccggggtga ggcacgccat cgtgctctgc atcgccttca
    38641 tcccccgcct cctggcatcg acatatatca agtaatgtcg gcccgggcgc cggcggccgg
    38701 cggcggctgg gttagcatcg gccgcatgcc gctcgagacc cactacgcgt ccggcctgct
    38761 gctgttcgcg ctgctcaacc cgttcctgat gagcgtgtac ctgatcgacc tgatcaccga
    38821 tctgccgcgc gccatgttcg tgcgcgtgct ggcgcgcggc gccctgatca gcgccctcgt
    38881 gttcgcggtg ttcgcctgga ccggcgacgc catcttcagc gacgtgctgc acgtgcgctt
    38941 cgcctcgttc caggtgttcg gcggcgtgat cttcctgctc atcggggtgc gcttcgtgtt
    39001 cagcggcgcc gatgccatgc gcgccatgcg cggccacccc gagcacatcg ccggctcgat
    39061 cgccatgccg ttcatgatcg gccccggcac ggtgagcgcg agcgtggtgg tgggcgcgcg
    39121 cctgcccgtg cccgacgcgg tgatggtgat cttcgccacc ctggcagtca cggtggcgct
    39181 ggtggcggcg atcaagttca cgcacgacta cgtgaaggag cgcaacgcac gcgtcgtcga
    39241 ccgctacatc gagatcgtcg ggcgcatctc ggcgctgctc atcggcacct tcgccatcga
    39301 gatgatcttc gagggcctcg gcacctggct cgaggacacc gcgccgcagt ggcgctagca
    39361 gggctctagc gcgcgccgat gcgcccggcg agcgggagct tgagcgccgg cggccaggga
    39421 tcgacgccga aggtgaggcc gagcaggttc acttccagcc cctccaccgt gctcgcggtg
    39481 atgccgaaca ggcccgcgag cgacatctgg tagccgctgc cgctcggcgc cgttgcgaac
    39541 acgccgccgc gcaggtagtc cttgccgatc gccgtggacg gcaggtcggc cttcagctcg
    39601 ggcacggcgc gcgtcaccca ggcggtgaag gtgttggaat tcggccccgg ccaggcgcgg
    39661 tactccttgc cgtagggata ggtctgcacc gcgccctcga tgcgcgcaat gagcggcgcg
    39721 gccgccgcgc cgcgcacgtc ggcgagcagc tcgggctcgt tgccgaacca gcgcgcgtcc
    39781 ggcgcgcgcg tgcgcaccga caccgaggtg tcgtcccagc gcagcttcca gccgatcacc
    39841 tcgtacaccg tgtaggccgg cgcgttctgc ggcttcaccg cgatccagct gtgcacgccg
    39901 aacaggccgc gcatgccgac ggcgcgcgcg gcgtagacct gcaccacggc ttcggcggcc
    39961 tgcgccggat cgggcgcgag gcccacgggc tcgcggctcg cggtgcgcca gtcggcgccc
    40021 gcgcgcgtca ccgcgcccac gccgatcgcg agcacgatcg ccaccaggcc ggcgccgagc
    40081 acgtgccggc gcctcaagcc ttctcccgcg actcgcgcca gatgtcgagc ttttcctgga
    40141 tcacgcggtc ggagtacagg aacagcacgg cgtccgcgga cgcctggtgc gtgtgcgcca
    40201 tccagctcgg cgcgacgaag atgtcgtgcg ggccccactc gaaggtctcg ccctggatgg
    40261 tgctgcggcc gcgcccctcg accaccgcga acacggtgct gtcggtggag cggtacggcg
    40321 cggtcttgaa gcccttcggc aggagctgca gccaggtgga gatcgtcggc atcgcccagc
    40381 tgccgttcac cgggttgatg tacttcatct tgtaggcgtg ccacgcgtcc gggtcgcgcg
    40441 tcctctgcag cgcgtcgagc gactcgcgcg acttgcggta cgggtagttg aagatcggcg
    40501 aggtctgcga gcggtgcacg gcgtccaccg gcagcaggtt gttgccgaac tcggcgggcg
    40561 ccgcgccctc ggggcgctcg agcttgtgcg cctttccgcc caggtcctcg cggaagctcg
    40621 ccgccagcat gttgacgatg tgcatgtcga ggccgtcgag ccacaccatg ggcccctcgc
    40681 cctcgtggcc gtggtcgtgc caggtccacg acggcgtgat gacgaagtcg cccggctgca
    40741 tcatcgtgcg ctcgcccgcc accgcggtga acgcgttgct gccttcgacg atgaacctga
    40801 gcgccgaggc cgtatgccgg tgcgccggcg cgatctcgcc cggcaagatg atctgcagcc
    40861 cggcaaacag gctctgcgtg atgcgcgact ggccggcgag gcccgggttc tccagcacca
    40921 ttacgcggcg ctcggcttcc gccgccccga tcagctcgca cgcttccatc aggtgcgggc
    40981 ggacatcggc gtagtgccag atcgccggca ccgcgcgcgt cgcgggcttg tcggtcacca
    41041 ggccgtgcag cacgcgccac agcggcgcga ggtgctgctt cttcgcgcgc gcgtagaagt
    41101 ccttgcgccc ttcctcggcg gcgaggttca ccgtgggcac gatgccctcg agctcttcgg
    41161 cctcgcggcg cgcgccttca accggtttca tcaacggctc ccagactaaa gtcgaacagc
    41221 ttgcggtcca tgaggtgcga ggggcgcacg tcgccgagcg cccgcaggat cgattctacg
    41281 cgcccggggt gcgccttttc ccactgccgc agcatgtcgc gcaccacgcg ccgctgcagg
    41341 tgctcctgcg agccgcacag gttgcacggg atgatgggaa agcccttttc ctcggcgtag
    41401 gcggcgaggt cgtcctcgcg cacgtaggcg agcggccgga tcagcacgtg gcggccgtcg
    41461 tccgagcgca gcttgggcgg catcgctttc agcttgccgc cgaaaaacag gttgaggaag
    41521 aacgtggcga ggatgtcgtc gcggtggtgc ccgagcgcga tcttggtggc gccgagctcg
    41581 cccgcgacgc ggtacagcac gccgcggcgc agccgcgagc acagcgagca catggtcttg
    41641 ccctcgggca gcacgcgctt caccaccgag taggtgtcct gctcgacgat gcggaacggc
    41701 acgccgagcc ccgccaaata gtcgggcagc acgtgcgccg gaaagccggg ctgcttctgg
    41761 tcgaggttga cggcgacgag ctcgaagggc acgggcgcct tttcgcgcag cgccatcagc
    41821 acgtcgagca tggcgtagct gtccttgccg cccgagaggc acaccatcac ccggtcgccc
    41881 gcctcgatca tgccgaagtc ggcgatcgcc tcgcccacct ggcgcctgag gcgcttggcg
    41941 agcttgccgg cctcgtaggc agctttgcgc gggtcgcgct gcagacggac gacgtgcatc
    42001 gggagtctca gagaaagatg gagcggccgc cgtccaccgc cagtacctgg ccggtgacga
    42061 agggcgcacc gcacaggaag tgtaccgcct gcgccacgtc ctcggcggcg ccggtgcgcg
    42121 cgagcggcgt cgtggcgacg atgcgcttgc gttcgtcggg cgcgaactgc ccgtcctcgg
    42181 gccaggcgat ggcgccgggc gccactgcgt tgacgcgcac cccgggtccg agctcgagcg
    42241 cgagcgcgcg cgtcagcgcg acgagccccg ccttcgccac gctgtagacg acgtagtcct
    42301 tgagcggccg ctcggcgtgg atgtcggcga tgttgacgat ggcgccgccg gcaagcgcaa
    42361 gctgcggcgc cgccgcctgc gcgagaaaca gcggcgcgcg caggttcgag ccgatcagct
    42421 cctcccagtg gctcgcctcg atctcgccga gcggcgtggg atagaacgcg gaggcattgt
    42481 tgacgaggaa gtccagccgc gcgaagtgct gctgcgccgc gctcaccagc gcctgcggcg
    42541 cgaccggcgc gagcaggtcg cacttcacct tgatcgcgct gccgcggcgc agcacgttga
    42601 ggtccttttc cagctgcgcg gcgtcggttt cggcgccgcg atagtggatc acgatgctcg
    42661 cgcccgcggc atgcagccgg cgcgcgatcg ccgcgcccac gcggcgcgcc gcgcccgtca
    42721 ccagcgccac cttgctctcg agggaagcgt ccgtcacccc ggggattcta ggttaaattc
    42781 gccgcatgct gcccgagccc gacgccgagg cgcgcgccgc gagcgcgcgc ctcgccgagc
    42841 gtatccgcgc cgagatcgcc gccggcggcg gctggctcgg cttcgtgcgc tacatggagc
    42901 tcgcgttgca cgcgccgggc ctgggctact acgcgggcgg cgcctcgaag ctcggcgccg
    42961 cgggcgactt cgccaccgcg ccggagctgg ggacgctctt cgcgcgcacg ctcgcgcgcc
    43021 agttggccga ggtgctcgcg cccggcgagg cgatcctgga gttcggcgcg ggcagcggcg
    43081 cgctcgccgc ggcgctcatc gccgagctcg cgcccgagcg ctacctgatc ctcgagacga
    43141 gcgcagagct cgccgcgcgc cagcgcgcgc gcctcggcgc tcgcgcgcaa tggatcgagc
    43201 gcctgcccga gcgcttccgc ggcgtgatga tcgccaacga ggtcgccgac gcgatgccgg
    43261 tgcacgcgct cgcctggcgg cgcgagggcg tgatggagcg cggcgtgcgc gtcgccggcg
    43321 gcgccttcgc ctgggacgag cgcccggcca cgggcgagct gcgcgaggcg gcgcgccggc
    43381 tcgccgtgcc cgtgccctac gagagcgaga tcgggctcat ggcgcgcgcc tggatgaagc
    43441 tcgtcgcgcc gcgcctggag cgcggcgcgc tcttcgtcat cgactacggc tttcccgagc
    43501 gcgagtacta ccacccgcag cgcgccatgg gcacgctcat gtgccattac cgtcaccgcg
    43561 cgcatggcga tgtgttcttc catccgggcc tgcaggacat caccgcgcac gtggacttca
    43621 cggcgctcgc gcgcggcgcg cgcgaggccg ggctcgaggt gctcggctac gccaaccagg
    43681 ccggctttct cgtcgattgc ggcatcaccg aggtgctggg gcgggaggac ccggaggacg
    43741 tggcgcgcta tgcgccgcgc gccgccgagg cgcagatgct gctctccccc gccgagatgg
    43801 gcgagctatt caaggtcctc gcggtgggcc gcggggtgcc gcggccgctc ctcggctttg
    43861 cgaattgcga ccgatcgcat acgctctagg tccatgcgac tgatcgccgt cctcgccctc
    43921 gcgctgcccc tggcggcgct cggcgacgac atcgccgagg cgaagaagcg ctgggccgaa
    43981 agcccgcacg ggccgctgct ggagcgcatc ctgccgccga ccttcgagcg cgcgcagctc
    44041 ccggagccgc gcgcgcgcgg cgcgcgcctg accgtgcagt actgcgtgca gtgccacaac
    44101 ctgcccaatc cggcgatgca tcacgccgag aagtggccga ccatcgtcga gcgcatggtg
    44161 ctgaggatgg agggcaaagg caattacggg cagctgatgt tcgacatgat ggccggcgtg
    44221 aaggcgccga gagcgccgga aaccgatgcg ctggtggcgt acctgcaaaa gcactcgcag
    44281 aagccgctcg atccgcgcaa ggtgcccgag gcctacaccg ccgcgggaga gccgttccgg
    44341 ctcgcctgca accagtgcca tgtgctcccc gaccccaggc gctacacggc gaaggagtgg
    44401 ccggcggtag tctcgc
//
LOCUS       DBNHKE010000124         3832 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1237805, whole genome shotgun sequence.
ACCESSION   DBNHKE010000124 DBNHKE010000000
VERSION     DBNHKE010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1237805"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="AC16O9_07970"
     CDS             complement(<1..1303)
                     /locus_tag="AC16O9_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6516592.1"
                     /translation="MLNRDKFFIGGQWVAPSSRETIEVHHSGTGEVMGEVPAGTERDI
                     DAAAAAAAAALPSWSATSAEERAGCLQKISEGLKARSEEIAKTIAAEVGMPIKLAGRI
                     QVGLPIANFANYARIAREFKFEERVGNSLVVKDPVGVVGAITPWNYPLHQITLKLAPA
                     LAAGCTVVLKPSEVAPFNAFILAEVIEAAGLPKGVFNLVTGLGPVVGEALVKHPKVDM
                     ISFTGSTRAGKRISEVAAQQVKRVALELGGKSASVILEDADLAAAVKGTVNGCYLNSG
                     QTCTALTRMLVPASRHDEIAKLAVEAAKGFTLGDPLSESTRLGPLASHLQLERVRGYI
                     RKGVEEGAELLAGGAEPPEGVPAGYFVKPTIFGKVKNSMTIAQEEIFGPVLSIIPYQD
                     EEEAVKIANDSPYGLAGAVWSKDEARAQAVARRIRAGQIDVN"
     gene            1481..2566
                     /locus_tag="AC16O9_07975"
     CDS             1481..2566
                     /locus_tag="AC16O9_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6516593.1"
                     /translation="MICRELNKPVVVEPVSVDAPKRGEVTVKIAACGVCHSDLSATNG
                     TIAMPPPLVLGHEAAGEVIEVGEGVTGLAVGDHVVSSFIYMCGRCRFCAAGRPVLCIE
                     QGKALTTPPEGTPRVKDAAGKPLGIFSGCGVMAEYATLSVDNLVRIDPKIPLDRAALV
                     GCAVTTGVGAVFNTAKVEPGSSVAVFGCGGVGLNVVQGAAIAGAERIIAIDTLPEKLE
                     MAKRFGATDTVLAKPGEDTAKALKKMTGGGPDYAFECVGAGVLAETAYRAIRRGGKAV
                     IVGVARPTEAASFRPMSMVFEEKTLQGSYFGSSVPRIDFPKMLQLYMAGRLKLDELIT
                     HRYKIDEAPQAFADLESGRNARGVIVF"
     gene            2566..3699
                     /locus_tag="AC16O9_07980"
     CDS             2566..3699
                     /locus_tag="AC16O9_07980"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6516594.1"
                     /translation="MASIVAARRGRILVVDDQRANVEMLAELLRSRGYLVDGAYDGES
                     ALAKIAEMKPDLVISDIRMPGMDGYELTQRLRGEPATELLPVVLVTSLEDRDGHDERV
                     KGIESGADDFLTKPVRYAELFARVRSLLRVKVLQDEVRRQATELREWNERLEERVKEQ
                     VAAIDRLAKMKRFFSPPVADAIIAGGVDMLEPHRREITAVFLDLRGFTAFTDRADPDE
                     VMDLLSDYHETMGNLVERYGGTLEHFAGDGVMIFFNDPIPVEQPAHKAVRMAIDLLHA
                     FDPIARTWKSRGYEVALGVGIAQGEATLGVIGFEQRWEYAAIGAVPNLAARLCGQAKG
                     GEILVDSATCSAVGDAVDLEPVGPMSLRGLTQPIKAFRVKGVR"
BASE COUNT          577 a         1290 c         1371 g          594 t
ORIGIN      
        1 cgttcacgtc gatctggccc gcgcggatgc gccgcgccac ggcctgcgcg cgcgcctcgt
       61 ccttggacca caccgcgccg gcgaggccgt agggcgaatc gttggcgatc ttcactgcct
      121 cctcttcgtc ctggtacggg atgatggaga gcaccggccc gaagatctcc tcctgggcga
      181 tggtcatgga gttcttcact ttgccgaaga tggtgggctt gacgaagtac ccggccggga
      241 cgccctcggg cggctcggcg ccgccggcga ggagctcggc gccttcctcg acgcccttgc
      301 ggatgtagcc gcgcacgcgt tcgagctgca ggtgcgaggc gagcggcccg aggcgcgtcg
      361 attcggacag cgggtcgccc agcgtgaagc ccttggccgc ctcgaccgcg agcttcgcga
      421 tctcgtcgtg cctggaagcg ggcacgagca tccgcgtgag cgcggtgcag gtctgccccg
      481 agttgaggta gcagccgttc accgtgccct tcaccgcggc ggcgaggtcg gcgtcctcca
      541 ggatcacgga ggcgctcttg ccgcccagct cgagcgccac gcgcttcacc tgctgcgctg
      601 cgacctccga gatgcgtttt cctgcgcgcg tcgagccggt gaaggagatc atgtccacct
      661 tcggatgctt gacgagcgcc tcgcccacca cgggccccag gccggtgacg aggttgaaga
      721 cgcccttcgg cagccccgcg gcctcgatca cctcggcgag gatgaatgcg ttgaacggcg
      781 cgacctcgga gggcttgagc accaccgtgc agcccgcggc gagcgcgggc gcgagcttga
      841 gcgtgatctg gtgcagggga tagttccagg gcgtgatcgc gccgaccacg cccacggggt
      901 ccttcacgac gagcgagttg ccgacgcgct cctcgaactt gaactcgcgc gcaatccggg
      961 cgtagttggc gaagttcgcg atcggcaggc cgacctggat ccggccggcg agcttgatcg
     1021 gcatgccgac ctcggcggcg atggtcttcg cgatctcctc cgagcgcgcc ttcaggccct
     1081 cggagatctt ctgcaggcag cccgcgcgct cctcggcgct ggtcgcgctc caggacggca
     1141 gggcggctgc cgccgcggcc gccgcggcgt cgatgtcgcg ctcggtgccc gcgggcactt
     1201 cgcccatcac ctcgccggtg ccggagtggt gcacctcgat cgtttcccgg gaagaagggg
     1261 cgacccattg gccgccaatg aagaacttgt ctcgattgag catgggatca cccgtggacg
     1321 gcaagagcgg tactttaacg ccccgccgcg gcccgcggca acgagacgat gcgcgcggcc
     1381 cgggctgcgg aactgcgggg cgcgcctcgg tgcaggccgc tatactcgag gcgatctgac
     1441 gcagagattg gggggctgca ggtcccgcgc cggcaaggca gtgatctgtc gcgaactgaa
     1501 caagcccgtg gtcgtcgagc cggtgagcgt cgacgcgccc aagcgcggcg aggtgacggt
     1561 gaagatcgcc gcctgcggcg tgtgccattc ggacctctcg gccaccaacg gcaccatcgc
     1621 gatgcccccg ccgctcgtgc tcgggcacga ggccgcgggc gaggtgatcg aggtgggcga
     1681 gggcgtgacg ggcctcgccg tcggcgacca cgtcgtgtcc tcgttcatct acatgtgcgg
     1741 caggtgccgg ttctgcgccg ccggccgccc ggtgctgtgc atcgagcagg gcaaggcgct
     1801 caccacgccg cccgaaggca cgccgcgcgt gaaggatgcc gcgggcaagc cgctcggcat
     1861 cttctcgggc tgcggggtga tggcggagta cgccacgctg tcggtggaca acctcgtcag
     1921 gatcgatccc aagatcccgc tcgatcgcgc ggcgctggtc ggctgcgccg tgaccaccgg
     1981 cgtgggcgcg gtgttcaaca cggcgaaggt cgagccgggc agtagcgtcg ccgtgttcgg
     2041 ctgcggcggc gtcgggctca acgtggtgca gggcgcggcg atcgccggcg ccgagcgcat
     2101 catcgccatc gacaccctgc cggagaagct cgagatggcc aagcgcttcg gcgccaccga
     2161 cacggtcctc gccaagccgg gcgaggacac ggccaaggcg ctgaagaaga tgaccggggg
     2221 cggccccgac tacgccttcg agtgcgtggg cgcgggcgtg ctggccgaaa ccgcgtaccg
     2281 cgcgatccgg cgcggcggca aagcggtgat cgtgggggtg gcgcgcccga ccgaggccgc
     2341 ctccttcagg cccatgagca tggtgttcga ggagaagacc ctgcagggca gctacttcgg
     2401 ctcctcggtg ccgcgcatcg atttcccgaa gatgctgcag ctctacatgg cggggcgcct
     2461 caagctcgac gagctgatca cgcaccgcta caagatcgac gaggcgccgc aggcgttcgc
     2521 cgacctcgaa tccggccgta acgcgcgggg cgtgatcgtc ttctgatggc atcgatcgtc
     2581 gccgcgcgcc gcggccgcat cctggtggtg gacgaccagc gcgcgaacgt cgagatgctg
     2641 gccgaactgc tgcgctcgcg cggctacctc gtcgacggcg cctacgacgg cgagtccgcg
     2701 ctggcgaaga tcgccgagat gaagcccgac ctcgtcatct ccgacatccg catgccgggc
     2761 atggacggct acgagctcac gcagcgcctg cgcggcgagc ccgccaccga gctcctgccg
     2821 gtggtgctgg tgacctcgct cgaggaccgc gacgggcacg acgagcgcgt caagggcatc
     2881 gaatcgggcg cggacgactt cctcaccaag ccggtgcgct acgcggagct gttcgcgcgc
     2941 gtgcgctcgc tgctgcgcgt caaggtgctg caggacgagg tgcgccgcca ggccacggag
     3001 ctgcgcgagt ggaacgagcg gctggaggag cgcgtgaagg agcaggtggc ggcgatcgac
     3061 cgcctggcga agatgaagcg cttcttctcg ccgccggtcg ccgacgccat catcgccggc
     3121 ggcgtcgaca tgctcgagcc gcaccggcgc gagatcaccg ccgtgttcct cgacctgcgc
     3181 ggcttcaccg cgttcaccga tcgcgccgat cccgacgagg tgatggacct gctctcggac
     3241 taccacgaga caatgggcaa cctcgtcgag cgctacggcg ggaccctgga gcacttcgcc
     3301 ggcgacggcg tgatgatctt cttcaacgac ccgataccgg tggagcagcc cgcgcacaag
     3361 gcggtgcgca tggcgatcga tctgctgcat gccttcgacc ccatcgcccg gacctggaag
     3421 agccgcgggt acgaggtggc gctcggggtg ggcatcgccc agggcgaggc gacgctcggg
     3481 gtcatcggct tcgagcagcg ctgggaatac gccgcgatcg gcgcggttcc caatctcgcg
     3541 gcgcgcctgt gcggccaggc gaagggcggc gagatcctgg tggattccgc gacgtgcagc
     3601 gccgtggggg acgccgtgga cctcgagccc gtcgggccga tgagcctgcg cggcttaacc
     3661 cagccgatca aggcgtttcg ggtgaagggg gtgcggtaaa taggtaaata gggacagacc
     3721 tcatttatcc agtctgcgct tggtgcgcag acgtcgacaa atgaggtctg tccctattta
     3781 cagccgagct tggcttccct cgccttgacc agcgcggtga tggtttcgtt ca
//
LOCUS       DBNHKE010000125         6697 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_814076, whole genome shotgun sequence.
ACCESSION   DBNHKE010000125 DBNHKE010000000
VERSION     DBNHKE010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_814076"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1833
                     /gene="msbA"
                     /locus_tag="AC16O9_07985"
     CDS             58..1833
                     /gene="msbA"
                     /locus_tag="AC16O9_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIR6516595.1"
                     /translation="MPHPRSHSSRELYLRLLTYVRPYTKAFALALAAMIATAATDPLF
                     PMLMKPLLDGGFAAAGSSDWPPIAFAVAIVGIFVLRGIFSFLSHYSMAWVADKVVLDL
                     RAAMFARLVRFPTRVFDDQSSGALLSRVAYDVANVSAAATTALTVIVRDSIVIVLLLA
                     WLFYLNWKLTLIALVIAPLVALFVRVLGRRLRNMARASQRALGDVVHVLEETIECHRV
                     VKVFGGQEYEGGRFARMIGALRGFNMRQKVPEALITPVTHLLAASAMAVIVYVALREA
                     LAAETTVGEFASFFTAMLMLFAPIKHLTQVNSALQRGLAAAESVFGMIDAPVEEDRGT
                     VVLARARGEIAFENVTFTYETREAPALREVSLQVRPGETLALVGASGSGKTTLVNLLP
                     RFYAPEGGRITLDGHDIQTLTLESLRGNLALVSQDIVLFNDTIYANIAYGRLSGASEK
                     EVVAAAEAAHALSFIRETPEGLNTFIGENGLRLSGGQRQRLAIARALLKDAPVLILDE
                     ATSALDTESERHVQAALEALMRGRTTIVIAHRLSTIERADRIAVLDGGRVVESGRHAE
                     LLARGGLYARLHRVQFAAAPLAATA"
     gene            1841..2845
                     /gene="rfbB"
                     /locus_tag="AC16O9_07990"
     CDS             1841..2845
                     /gene="rfbB"
                     /locus_tag="AC16O9_07990"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR6516596.1"
                     /translation="MLLVTGGAGFIGSNFVLSTLAAAGEPIVNLDKLTYAGNAANLDA
                     LRGDARHVFVKGDICDRALVRQLLERHRPRAVVHLAAESHVDRSIEGPAEFVQTNVVG
                     TFALLEEARSYWQSLPAAARAAFRFLHVSTDEVYGSLGAEDPAFSETTPYAPNSPYSA
                     SKAASDHLVRAYWHTYGLPTLTTNCSNNYGPHQFPEKLIPLMIHQALAGKPLPVYGDG
                     QNVRDWLYVLDHCAALRVVLERSRPGEAYNIGGGAEMKNIDLVKTLCALLDDARPRTG
                     GGYADLISFVKDRPGHDRRYAIDSRKIQRELGWKPEESFQSGLKKTVRWYLECRVAVF
                     "
     gene            2867..3331
                     /locus_tag="AC16O9_07995"
     CDS             2867..3331
                     /locus_tag="AC16O9_07995"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6516597.1"
                     /translation="MQPDHTIPVSDLARRTREVVERLVREPGTRYVVMKNNRPVAVIA
                     GIERGAPPASQRRAGLREEVLRRKTLVAALARAHGARTVQLFGSAARGEERPDSDIDF
                     LVELEPGRSLIDVIGLENDLSDLFGRKVDVVAKQSAKPRVLAGSLKDAVRIV"
     gene            3335..3799
                     /locus_tag="AC16O9_08000"
     CDS             3335..3799
                     /locus_tag="AC16O9_08000"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: protein motif:HMM:NF014043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR6516598.1"
                     /translation="MARPAFDRLADVLEAIEKIREYTRGDEAAFRRNSMARDAVVARL
                     IHIGQAIKDAQEGGLDLPTLAPQVPWRSIAGMRDRLVHKYWDVDHAIVWNVISSELGN
                     LERAVKEIASRTKRGARTGKGADAVRRKRASRGIRERDVSAAAAGSRKRGHK"
     gene            3813..4688
                     /gene="rfbA"
                     /locus_tag="AC16O9_08005"
     CDS             3813..4688
                     /gene="rfbA"
                     /locus_tag="AC16O9_08005"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR6516599.1"
                     /translation="MKGIILAGGSGTRLYPVTQVVSKQLLPVYDKPMVYYPLTTLMLA
                     GIRDILVISTPQDTPRFADLLGNGRRWGIKLSYAVQPKPEGLAQAFLIGKKFIGKDAV
                     ALVLGDNLFHGHDLSALLSRAARRKRGATVFAYPVHDPQRYGVVEFDAKGKAVSLEEK
                     PKKPKSRYAVVGLYFYDNRVVRIAEGLKPSARGELEITDVNRAYLDRGTLEVVPMGRG
                     YAWLDTGTHESLLEASHFIETIEKRQGLKVACPEEIAYRQGYISARQLKTLARPLAKS
                     GYGEYLLRIVQEQVF"
     gene            4688..5233
                     /gene="rfbC"
                     /locus_tag="AC16O9_08010"
     CDS             4688..5233
                     /gene="rfbC"
                     /locus_tag="AC16O9_08010"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR6516600.1"
                     /translation="MQVLPTDLPGVLLLEPRVFGDARGYFYEAYNRRTFHKATGVDVE
                     FVQDNHSRSSKNVLRGLHYQVRQAQGKLIRCTAGEVWDVAVDLRKGGQTFGKWVAFTL
                     SAESKRMAWIPPGFAHGFLVVSDVAEVLYKTTDYYSPEHERTLLWSDPALAIKWPLAG
                     APVLADKDKRGTPLAAAEVFA"
     gene            5230..6069
                     /gene="rfbD"
                     /locus_tag="AC16O9_08015"
     CDS             5230..6069
                     /gene="rfbD"
                     /locus_tag="AC16O9_08015"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR6516601.1"
                     /translation="MRILLTGATGQVGWELRKTLAPLGEVRVFDRFGLDLADAPVLVA
                     TVRALQPDVVVNAAAYTAVDKAEAERDLAFAVNATAPRVLAEEARRIGALLVHYSTDY
                     VFDGEKADPYVEEDATRPINAYGASKLAGEQAILASGCRHLILRTSWVYGPRGKNFYL
                     TMLRLARERPELKVVDDQVGAPTSSLEIARATATLLAKGSQGLYHMSAAGETTWCGFA
                     RAILERAGSATPVVAIRTAEYPTPAKRPRNSRLDCSKLRAEHGVSLARWERALDEVTS
                     APR"
     gene            6066..>6697
                     /locus_tag="AC16O9_08020"
     CDS             6066..>6697
                     /locus_tag="AC16O9_08020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR6516602.1"
                     /translation="MTKKVALITGITGQDGAYLAEFLLAKGYVVHGIKRRASSFNTQR
                     IDHLYQHPHEPDRRLILHYGDMTDSSSLTRIVQQSQPDEIYNLAAQSHVAVSFEEPEY
                     TADSDGVGTLRLLEAIRILGMEKKTRFYQASTSELYGKVVETPQKETTPFYPRSPYGV
                     AKLYAYWITVNYRESYGMFACNGILFNHESPIRGETFVSRKITRALARIKL"
BASE COUNT         1093 a         2260 c         2310 g         1034 t
ORIGIN      
        1 ggatgcgcat ggccgtgctc tgctggagaa agtagcgcga gccgctacaa tccgcccatg
       61 ccccacccgc gcagccacag cagccgggag ctgtacctgc gcctgctgac ctacgtccgg
      121 ccctatacca aggccttcgc gctcgcgctc gccgccatga tcgcgaccgc ggccaccgac
      181 ccgctctttc ccatgctcat gaagccgctg ctcgacggcg gcttcgcggc cgccggcagc
      241 tcggattggc cgccaatcgc ttttgcggtg gcgatcgtgg gcatcttcgt cctgcgcggc
      301 atcttcagtt ttctgtcgca ctacagcatg gcctgggtgg cggataaggt ggtgctcgac
      361 ctgcgcgccg cgatgttcgc gcggctggtg cgctttccga cgcgcgtgtt cgatgaccag
      421 agctcgggcg cgctgctttc gcgcgtggcc tacgatgtcg ccaacgtgag cgctgcggcc
      481 acgacggcgc tcaccgtgat cgtgcgcgat tcgatcgtca tcgtgctgct gctggcgtgg
      541 ctgttctacc tcaactggaa gctgacgctg atcgcgctcg tcatcgcgcc gctggtggcg
      601 ctcttcgtgc gcgtgctggg gcggcggctg cgcaacatgg cgcgcgcttc gcaacgggcg
      661 ctcggcgacg tggtgcacgt gctcgaggaa acgatcgagt gccaccgggt ggtgaaagta
      721 ttcggcgggc aggagtacga gggcgggcgc ttcgcgcgca tgatcggcgc gctgcgcggc
      781 ttcaacatgc gccagaaggt gcccgaggcg ctgatcacgc cggtgacgca cctgctcgca
      841 gcctcggcga tggcggtgat cgtgtacgtc gcgctgcgcg aggcgctggc ggccgagacc
      901 acggtgggcg agttcgcgtc gttcttcacc gccatgctga tgctgtttgc accgatcaag
      961 cacctgacgc aggtgaactc ggccctgcag cgggggctcg ccgcggccga gagcgtcttc
     1021 ggcatgatcg atgccccggt cgaggaggat cgcggcacgg tggtactcgc gcgcgcgcga
     1081 ggcgagatcg cctttgagaa cgtgaccttc acctacgaga cgcgcgaagc acccgcgctg
     1141 cgcgaggtca gcctgcaggt gcgcccgggc gagacgctgg cgctcgtcgg cgcctcgggc
     1201 agcggcaaga cgacgctcgt caacctgctg ccgcgcttct acgcgccgga aggcggccgc
     1261 atcacgctcg acgggcacga catccagacg ctcaccttgg aaagcctgcg cggcaacctc
     1321 gcgctcgtgt cgcaggacat cgtgctcttc aacgacacga tttacgccaa catcgcctac
     1381 ggacgcctga gcggcgcgag cgagaaggag gtggtcgcgg cggcggaagc cgcgcacgcc
     1441 ctgagcttca tccgcgagac gccggaaggg ctcaacacgt tcatcggcga gaacggcctg
     1501 cggctttcgg gcggccagcg ccagcgcctc gcgatcgcgc gcgcgctcct caaggacgcg
     1561 ccggtgctga tcctcgacga ggcgacctcg gccctcgaca cagagtccga gcggcacgtg
     1621 caggcggcgc tcgaggcgct gatgcgcggc cgcaccacca tcgtcatcgc gcaccggctc
     1681 tccacgatcg agcgcgccga ccgcatcgcg gtgctggacg gcgggcgcgt ggtggagtcc
     1741 gggcggcacg ccgagctgct cgcgcgcggc gggctgtatg cgcggctgca ccgcgtgcag
     1801 ttcgcggccg cgccgctcgc cgcgacggca taatcgcgcc atgctcctcg tcacgggcgg
     1861 cgccgggttc atcggctcga atttcgtcct ttccaccctc gccgccgcgg gcgagccgat
     1921 cgtcaacctc gacaagctca cctacgccgg caatgccgcg aacctcgacg cgctgcgggg
     1981 cgatgcgcgc catgtcttcg tcaagggcga catctgcgac cgcgcgctcg tgcgccagct
     2041 cctcgagcgc catcggccgc gcgccgtggt gcacctcgcc gcggagagcc acgtcgaccg
     2101 ctcgatcgag ggtccggcgg agttcgtgca gacgaacgtc gtcggcactt tcgcgctcct
     2161 cgaagaagcc cggagctact ggcagtcgct ccccgcggcg gcgcgcgccg cgttccgctt
     2221 cctgcacgtg tctaccgacg aggtctacgg ctcgctcggg gccgaggacc cggcgttcag
     2281 cgagaccacg ccctacgccc cgaacagccc ctacagcgcc tcgaaggcgg cctccgacca
     2341 cctggtgcgc gcctactggc atacctacgg gctgcccacg ctcaccacca actgctcgaa
     2401 caactacggc ccgcaccagt tccccgagaa gctgatcccg ctcatgatcc accaggcgct
     2461 cgcgggcaag cccttgccgg tgtacggcga cgggcagaac gtgcgcgact ggctctacgt
     2521 gctcgatcac tgtgccgcgc tgcgcgtggt cctcgagcgc agccgccccg gcgaggcgta
     2581 caacatcggc ggcggcgccg agatgaagaa catcgacctg gtgaagacgt tgtgcgcgct
     2641 gctcgacgat gcgcggccgc gcaccggcgg cggctacgcg gacctgatca gcttcgtcaa
     2701 ggaccggccc gggcacgacc ggcgctacgc catcgattcg cgcaagatcc agcgcgagct
     2761 cggctggaag cccgaggaga gcttccagtc gggcctgaag aagaccgtgc gctggtacct
     2821 ggagtgccgg gttgcagtgt tctgaaaatt ttcagataat cgcccgatgc agcccgacca
     2881 cacgatcccg gtctccgacc tcgcgcgccg cacgcgcgag gtcgtcgaac gcctggtgcg
     2941 cgagccggga acgcgctacg tcgtcatgaa gaacaaccgc cccgtggccg tcattgccgg
     3001 aatcgagcgc ggcgcgccgc cggcaagcca gcgtcgcgcc ggcctgcgcg aggaggtgtt
     3061 gcgcaggaag acgctggtcg ccgcgctggc gcgggcacac ggcgcccgca cggtccagtt
     3121 gttcggctcg gcggcgcgcg gcgaggaacg gcccgacagc gacatcgatt tcctcgtgga
     3181 gctggagccc ggccgaagcc tgatcgacgt catcgggctg gagaacgatc tctcggacct
     3241 gttcggccgc aaggtggacg tcgtggcgaa gcagtcggcg aagccgcggg tgctggcggg
     3301 ctcgctgaag gacgccgtca ggatcgtctg agcgatggcg cgcccggcct tcgatcgcct
     3361 ggcggacgtg ctcgaggcga tcgagaagat ccgcgaatac acgcgcggcg acgaggcggc
     3421 ttttcgcagg aattcgatgg cgcgggatgc cgtggtggcg cggctcatcc acatcggtca
     3481 ggcgatcaag gatgcgcagg agggcggcct cgatctgccg acgcttgcgc cgcaggtgcc
     3541 gtggcgcagc atcgccggca tgcgcgatcg cctggtgcac aagtactggg acgtggatca
     3601 tgcgatcgtg tggaatgtga tctcatcgga gctgggcaac ctggagcgcg ccgtgaagga
     3661 aatcgcttcg agaacaaagc gcggcgcgcg caccggcaag ggcgccgacg cggtgcgccg
     3721 gaagcgcgct tcgcgcggca tccgggagcg ggacgtctcg gccgcggcgg cagggtcgcg
     3781 caagcgtggg cacaagtaat gcacgacgag ccatgaaagg catcatcctc gcgggcggct
     3841 cgggcacgcg gctctatccg gtgacgcagg tcgtttccaa gcagctcctg cccgtgtacg
     3901 acaagccgat ggtgtactac ccgctcacca cgctgatgct cgcgggcatc cgcgacatcc
     3961 tcgtcatctc cacgccgcag gacacgccgc gctttgccga cctgctcggc aacggccgcc
     4021 ggtggggcat caagctctcg tatgcggtgc agccgaagcc cgaaggcctc gcgcaggcgt
     4081 tcctcatcgg caagaagttc atcggcaaag acgcggtggc gctcgtcctg ggcgacaacc
     4141 tgttccacgg gcacgatctg tccgcgctgc tctcgcgtgc agcgaggcga aagcggggcg
     4201 cgacggtgtt cgcctacccc gtgcacgacc cgcagcgcta cggcgtggtc gaattcgacg
     4261 ccaagggcaa ggccgtgagc ctggaagaga agccgaagaa gcccaagtcg cgctacgcgg
     4321 tcgtgggact gtatttctat gacaaccggg tggtgcggat cgccgagggg ctcaagccct
     4381 ccgcgcgcgg cgagctcgag atcaccgacg tgaaccgtgc gtacctcgac cggggaacgc
     4441 tcgaagtcgt gccgatgggg cgcggctatg cctggctcga caccgggacg cacgaatcgc
     4501 tgctggaggc ctcgcatttc atcgaaacca tcgagaaacg ccagggcctg aaggtcgcct
     4561 gcccggagga gatcgcctac cgccagggct acatcagtgc gcggcagctc aaaacgctcg
     4621 cccgcccgct tgcgaagagc ggctacggcg agtacctgct gcgcatagtg caggagcagg
     4681 tcttctgatg caggtgctgc cgacggacct tcccggcgtg ctgctgctcg agccgcgggt
     4741 gttcggcgac gcgcgcggct atttctacga ggcttacaac cgccgcacct tccacaaggc
     4801 gaccggcgtg gacgtggagt tcgtgcagga caaccactcg cgatcgagca agaacgtgct
     4861 gcgcgggctg cactaccagg tccgccaggc gcagggaaag ctgatccgct gtaccgccgg
     4921 ggaggtgtgg gatgtcgccg tcgacctgcg caagggcggg cagacattcg gcaagtgggt
     4981 ggcgttcacg ctgtccgcgg agtcgaagcg catggcctgg atcccgcccg gcttcgcgca
     5041 cggctttctg gtggtatcgg acgtggctga ggtgctctac aagaccacgg actactactc
     5101 gccggagcac gaacgcacgc tcctgtggag cgatccggcg ctggcgatca agtggccgct
     5161 tgccggcgcc ccggtgctcg ccgacaagga caagcgcggc acgccacttg cggccgcgga
     5221 ggtgtttgct tgaggattct gctgaccggg gccaccggcc aggttgggtg ggagttgcgc
     5281 aagaccctgg cgccgctcgg tgaggtaagg gtcttcgacc gcttcgggct cgaccttgca
     5341 gacgcgcccg ttctcgtggc gacggtgcgc gcgctgcaac ccgatgtcgt cgtgaatgcc
     5401 gccgcgtaca ccgcggtgga caaggccgaa gcggagcggg acctcgcctt cgccgtcaat
     5461 gccacggcgc cgcgagtgct ggccgaagag gctaggcgga tcggcgcgct cctcgtgcac
     5521 tactcgaccg actacgtgtt cgacggcgag aaagccgacc cctacgtgga ggaggatgcg
     5581 acccgcccga tcaatgcata cggtgcgtcc aagctcgcgg gggagcaggc gatcctggca
     5641 agcggctgcc ggcacctgat tctgcgcacg agctgggtgt acgggccgcg cgggaagaac
     5701 ttctacctga cgatgctgcg gctcgcgagg gagcggccgg agctcaaggt cgtggatgac
     5761 caggtgggag cgccgacttc cagcctggaa attgcccgcg ccacggcgac gctcctggcg
     5821 aagggcagtc aaggcctgta ccacatgagc gcggccgggg agaccacttg gtgcggcttc
     5881 gcgcgcgcga tcctcgagcg cgcggggagc gccacgccgg tggtcgccat ccgcaccgcg
     5941 gagtacccga cgccggcgaa gcggccgcgg aactcccggc tggactgctc gaagctgcgc
     6001 gcggagcacg gcgtgtcgct cgcgcggtgg gagcgggcgc tcgacgaggt aacatcggcg
     6061 ccacgatgac gaaaaaggtt gccctgatca ccggcatcac cggccaggac ggcgcgtatc
     6121 ttgccgagtt cctgctcgcc aagggctacg tggtgcacgg catcaagcgc cgtgcctcga
     6181 gcttcaacac ccagcgcatc gaccacctgt accagcaccc gcacgaaccg gatcggcgcc
     6241 tgatcctgca ctacggcgac atgaccgatt cgtcgagcct gacgcgcatc gtgcagcagt
     6301 cgcagcccga cgagatctac aacctcgcgg cgcagagcca cgtggcggtg agcttcgagg
     6361 agcccgagta cacggcggac tcggacggcg tcggcacgct acggcttcta gaggcgatcc
     6421 gcatcctcgg catggagaag aagacgcgct tctatcaggc gtccacctcc gagctctatg
     6481 gcaaggtggt ggagacgccg cagaaggaga ccacgccttt ctacccgcgc tcgccttacg
     6541 gcgtggccaa gctctacgcc tactggatca cggtgaatta ccgcgagtcc tacggcatgt
     6601 ttgcctgcaa cggcatcctc ttcaaccacg aatcgccgat ccgcggcgag acgttcgtgt
     6661 cgcgcaagat cacgcgggcg ctcgcgcgca tcaagct
//
LOCUS       DBNHKE010000126        15859 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_232782, whole genome shotgun sequence.
ACCESSION   DBNHKE010000126 DBNHKE010000000
VERSION     DBNHKE010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_232782"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..2519)
                     /locus_tag="AC16O9_08025"
     CDS             complement(36..2519)
                     /locus_tag="AC16O9_08025"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6516603.1"
                     /translation="MQGRCPQSSAAEEGERRRQARQRHLSACFHCGLPVAEPGRHLAP
                     VLGAERAFCCAGCEAVARAIAGAGFERYYAARSSAGARPVVLPPPAVYDDPAAQREFV
                     AAAGENVREATLILDRIRCAACLWLNEQTLRRVPGVLRADVNYATQRAQIAWNPQRTR
                     LSTIIEAIRAVGYDATPFDPRRSDALARRERRRALWRLFVAGFGAMQVMMYAWPAYVD
                     AGMGTLGADTAALLRWASLVITTPVLLFSCGPFFRGAWQDAKRRRLSLDTPIALGLAA
                     GYAASAWATFTGSGEVYFDSIAMLAFLLLAARTAELLARHKAGRELDRLARWMPSFAL
                     RLRGGTGERVPAHALVAGDRVRVAAGERIPADGVLEEGASSADESLLTGEARPVAKRA
                     GASLVGGSVNLEQPITLRVTRAGADTQAAAIGRLVERAAATRPALVESADRLAQHLTT
                     LVIATAGLAWALSGNVWIAVAVLVATCPCALALASPIVLTRAGAQLLARGAPLTRSRA
                     LEALERASDVVLDKTGTLTVGRPVLARAVLLADMDEAHCRALAGALEASSTHPLARAF
                     GATGPAPTLGGLRNEAGQGVEARVDGRRVRIGARRFCEQIAGAAPEARDAQVFLAEEG
                     RWLAAFTLEDALRPEAADVVRGLAARGLSVHLVSGDDPAIVGALAGRLGIARWQGGAT
                     PQDKLEYVTRLQRDGRVVAMVGDGLNDAPVLARADVSVAMGAGADAAQLQADVVLTAD
                     RLGALEELIAISRRALRLVRQNLGWALAYNAVALPAAALGGIGPWEAAIGMSASSFIV
                     VLNALRPLTREGTWKASTSSSPSPSPSYS"
     gene            complement(2444..2866)
                     /locus_tag="AC16O9_08030"
     CDS             complement(2444..2866)
                     /locus_tag="AC16O9_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6516604.1"
                     /translation="MGNVTRYNPFDELFNEFGKGFWVKPLAFPTGPEMQIKLDVKEDD
                     KGYTVHADIPGAKKEDIQVDIDGNVVSVRAETRQQKEEKKDEKVLYSERSYGMVSRSF
                     ALPVEVDEKAAKAEYKDGVLNLLLPKKANGGGKRVSVT"
     gene            3026..3472
                     /locus_tag="AC16O9_08035"
     CDS             3026..3472
                     /locus_tag="AC16O9_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6516605.1"
                     /translation="MFKHILLPVDGSKLALKGAKAGVRLAKALRARVTAVYVIPPFMP
                     VAYGDAAVYVPALSPLEYRKATEKGARKALAAVEADAKKARVRCKTRFLTAAQVYDGI
                     LRTARGAKCDAIAMASHGRGGLAGVLLGSETTRVLAHSKVPVLVLR"
     gene            complement(3535..3834)
                     /gene="sorU"
                     /locus_tag="AC16O9_08040"
     CDS             complement(3535..3834)
                     /gene="sorU"
                     /locus_tag="AC16O9_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorU family sulfite dehydrogenase c-type
                     cytochrome subunit"
                     /protein_id="HIR6516606.1"
                     /translation="MRAAAAGLAFALSCAAHGEELGRKVFTELAQPPCQLCHTLKAAG
                     AAGTVGPSLDELKPDLEQTLAAVRQGVGIMPPFSDKLTPEQIEAVARFVAKSAGK"
     gene            complement(3831..5030)
                     /gene="sorT"
                     /locus_tag="AC16O9_08045"
     CDS             complement(3831..5030)
                     /gene="sorT"
                     /locus_tag="AC16O9_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorT family sulfite dehydrogenase catalytic
                     subunit"
                     /protein_id="HIR6516607.1"
                     /translation="MTASLDRNRRKFLMTSAGGAAAAGLAGLAPSALAKGKPLPGYAA
                     WKDEANLIVHSSNTMETVRGAQGTSVITANEVLFVRNNLTPPDEAAVKNADAWQVEIQ
                     GVTNPRSFTLGELKALGVEAVPMVLQCSGNGRAFFGHKVGGTQWKTGAAGCSLWSGVP
                     MKAVVAAVGGVRQGMRYMTSTGGETLPAGVDPKRALVERSVPWNMLERAILAWELNGA
                     PLPVANGGPVRMIIPGYYGVNNVKYVKRVEFTADQTDARIQSSSYRVRPIGEKGAPGQ
                     PTMWDMNLKSFVTNPSGEDGQTVRAGMVQITGVAWSGTSAVKGVEVSLDGGASWRAAE
                     FFGPLLGPHAWRQFVLAVRLSPGTYTITSRAHAYDGEVQPELRVENERGYGHNGWRDH
                     AVKVTVA"
     gene            complement(5123..6253)
                     /locus_tag="AC16O9_08050"
     CDS             complement(5123..6253)
                     /locus_tag="AC16O9_08050"
                     /inference="COORDINATES: protein motif:HMM:NF022591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HIR6516608.1"
                     /translation="MTQPEVPAAPATAFERLRLRFAQNEREVVVAFFAGGFIFDIFTV
                     GRVDSWVTIAQQTLYLAAVTAVLLHMLLEQGAPPVELAGQPRIVHWYYEYRSAAIHFA
                     LGALMNLYAIFFFKSSSLLVSFGFLAFLLALLVANESRRVKAIGLHFKFALLGLCYLS
                     FAAIVVPIALGSIGTWVFVASMLAGVMPLAGVAWWVRRRRRALFGQVRRQMLLPLCAV
                     LLAFLAAYLLRVIPPAPLSIPFIGVYHGVERTERGYALAHERPWWRFWHHGDQDFRAQ
                     PGDRVYVFFRVFSPARFADRVRMRWYWEPGGRGWLLQDSIPIDIVGGREEGFRGYGYK
                     ANYQPGRWKVQVETEDGREIGRVYFTLESVPAEPRLLYTDLE"
     gene            6314..7684
                     /locus_tag="AC16O9_08055"
     CDS             6314..7684
                     /locus_tag="AC16O9_08055"
                     /inference="COORDINATES: protein motif:HMM:NF051235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6516609.1"
                     /translation="MQARSALAVLLLSLGLLAVPASSPAQPDKRVVHVAPIEGVIDLG
                     LAPFVRRAIDEAAQAGAAALVLEVNTFGGRVDAAVQIRDALLNSPVKTIAFVNKRAIS
                     AGALISLAAEHLVMSGGSTIGAATPVQAGAPGAAAQPVQEKTVSYVRKEFRATAESRK
                     RPPRLAEAMVDADVEIRGVIAKGKLLTLTTDEALKHKLADFRADTLESALEKLGLGGA
                     ELRRAAPNWAENVVRFLTHPVVSSLLISIAMIGILIELRTPGFGIPGAAGVTSLALFL
                     WGHWLVQLAGWEELLLALGGVVLLALELFVIPGFGIAGILGILALLAALVLSMLGTGD
                     TPQFMMLAATRVVFALLAALVASLVLLRYLPRLPFGRRFVLRTGLASAQGYASAPESD
                     RQWLGRKGRASSPLHPAGIAEIDGQRLDVVSDGELVEAGEFIEVIRVDSNRVVVRRIV
                     NPNAKE"
     gene            7688..8668
                     /gene="floA"
                     /locus_tag="AC16O9_08060"
     CDS             7688..8668
                     /gene="floA"
                     /locus_tag="AC16O9_08060"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HIR6516610.1"
                     /translation="MTGLETSLTGGLGLLILLVAGLALLLYLVPVPLWIAAWASGAYV
                     GLFTLIAMRLRRVPPSTMITARISSVKAGIDVSLNDLEAHFLAGGNVVRVVNAMISAD
                     KANIPLPFKRAAAIDLAGRNVLEAVQMSVLPKVIETPRIAAVAKDGIQLIVVSRVTVR
                     TNIDRLVGGAGEETIIARVGEGMVSTIGSATNHKSVLENPDHISKHILSKGLDAGTAY
                     EILSIDIADVDVGANIGAKLQIDQADADKQIAQAKAEERRAMAVALEQEMRARVVEAE
                     AEVPRAMAEAFRQGNLGIMDYYRMKNLQADTDMRGVIAGTGDDPAGAPKK"
     gene            8680..9063
                     /locus_tag="AC16O9_08065"
     CDS             8680..9063
                     /locus_tag="AC16O9_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516611.1"
                     /translation="MPKSGTELLFLGLVIAGFMLFNYVAQQLAKKARAQQEATEAEPA
                     AEAPAPAPEDEPLQDIWGRAPAKPPAAPEVVARAVPEPQAAPPLPSPGHPGVRLFRTR
                     KDLQRAIVLMTVLGPCRALEPHERR"
     gene            complement(9078..9311)
                     /locus_tag="AC16O9_08070"
     CDS             complement(9078..9311)
                     /locus_tag="AC16O9_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516612.1"
                     /translation="MRAVLATLARRREALVSRSAAQRAQVAAAACGVRQAVAEPLVLG
                     LGVAVALASGSPRLRAWLVRAWVIGAFVRRLLR"
     gene            complement(9308..9667)
                     /locus_tag="AC16O9_08075"
     CDS             complement(9308..9667)
                     /locus_tag="AC16O9_08075"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6516613.1"
                     /translation="MGLLQSVQGLLATLLGLARTRLELLSVEMQELLARLVLIMVGAV
                     AAILLAALALGFGAVALVMSVAPEHRVAVAAGLALAFLAAAALIAWRVRREARARPFA
                     ASLGELERDAKALEPRE"
     gene            complement(9670..9918)
                     /locus_tag="AC16O9_08080"
     CDS             complement(9670..9918)
                     /locus_tag="AC16O9_08080"
                     /inference="COORDINATES: protein motif:HMM:NF039524.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HIR6516614.1"
                     /translation="MDPATTEKLMADLRAVVRDAEGLLAAAAAESGERLGEAGAAAGD
                     SAREAAREIEDQVRRNPWAALGIAAGVGLVLGLLLGRK"
     gene            9961..12660
                     /locus_tag="AC16O9_08085"
     CDS             9961..12660
                     /locus_tag="AC16O9_08085"
                     /EC_number="2.3.1.-"
                     /EC_number="6.2.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HIR6516615.1"
                     /translation="MAGLSERHYLTPLFEPAAVAVIGATERAGAVGAVLTRNLLEARY
                     QGTLFAVNPKHRSVHGVPCFGAIGKVPGRVDLAVIATPAATVPDIIEQCGKAGVRAAV
                     VISAGFSETGPAGAKLERALLENARRHHLRLVGPNCLGLMRPALGLNATFARGAALPG
                     SLGLVAQSGAVCTAMLDWARPNGIGFSSVVSLGGSTDVDFGEIIDYLIGDPATEHILL
                     YIEGVRSARRFVSALRAAARVKPVIVQKVGRHPAGSRAAVSHTGAIVGLDDVFDAVVK
                     RAGVVRVKTIGQMVAAAQALAAHVRPRGDRLAVVTNGGGPGVMAADRAADLGIPLAEL
                     APATLEALKPALPANWSHGNPLDLIGDADAKRYRAAVSACLGDDGVDGVLAILTPQAM
                     TEPDAAAAALIEAARGSSKPVLACWMGDEQTAAARRRLAAARIPVFRTPDPAVDMFGH
                     VSAFYRNQRALLQTPGPVAAPDAPDLATARAIVDSALAARRSVLSEMESKALLAAFAI
                     PIARTVVTRSAHEAMLVAGEIGFPVAMKIDSPEITHKSDVNGVRLNLGNAQAVRATWE
                     EIVAEAKRLRPEAKVNGVTVEPMVRRAHGRELMVGVIRDPVFGPAIVFGTGGTAVEVH
                     RDRAVALPPLNAFLIADMIRGTRVAKLLGPFRRMPPVDMAALESILLRVSEMVCELPE
                     LAELDINPLIADASGAIAVDARAVLQPGAPQRDRYGHMAIHPYPAHLVGTWRPAEGPE
                     VTLRPIRPEDAQMEQAFVKSLSAETRYFRFMDTLRELTPQMLVRFTQIDYDREMAFVA
                     TVPEDGREAEIGVTRYVANPDGESCEFAVVIADGWQRRGLGRRMMEQLIDVARARGLR
                     AMVGHVLAENRGMLALCQKLGFGVADSAEGPIVKRVRLALTER"
     gene            complement(12665..13186)
                     /locus_tag="AC16O9_08090"
     CDS             complement(12665..13186)
                     /locus_tag="AC16O9_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR6516616.1"
                     /translation="MVDPIAAWHADHVRFGRLLDMLEKEVTLFHSGEQPNYELMRDIV
                     LWLRHYADGVHHPREDVAFERMVERDPALRLPVNRLLQEHRVIAYAGEALLERLENAA
                     NGGFAPRADLEAAAATFFAYYRSHMATEESTIMPRAAQLLQGADWAAVAAVVPREDAR
                     SAQALRVARNLGL"
     gene            13343..14677
                     /locus_tag="AC16O9_08095"
     CDS             13343..14677
                     /locus_tag="AC16O9_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR6516617.1"
                     /translation="MTVREGSLDAPTRHPLDWRNPEFYDEHKLDAELERVYDICHGCR
                     RCVNLCTAFPTLFDLIDESKTMEIDGVAKSDFWKVVDQCYLCDMCYMTKCPYVPPHPW
                     NVDFPHLMLRAKAVKFRRHGAGLRDRLLSSTDALGKLAAIPVVAQTVNAMNRLPAARG
                     VMEKMLGVHRERKLPDYASRKFRSTAAPSRARAVKDGKNTPGKVAIFSTCYVNYNEPG
                     MGHDLLALLEHNEIPYVLVEKEACCGMPKLELGDLEAVARLKDLNIPPLARLAREGYA
                     ILAPVPSCALMFKQELPLMFPDEAEVKRVQEAMFDPFEYFVTRAKDGLLKKDFKEKLG
                     KVSYHVPCHSRVQNMGQKTREMLEMVPGTELTTVERCAGHDGTWGVKREYFDNSMKIG
                     RPVFRQMAEPGPDYVSSDCPIAARHILQGMGDEAGKAAKAHPLTLLRKAYGI"
     gene            14709..15494
                     /locus_tag="AC16O9_08100"
     CDS             14709..15494
                     /locus_tag="AC16O9_08100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6516618.1"
                     /translation="MQLTPPPGAPFAFGVLGDTPYSAPQARALDGLIERINAQDLAFV
                     VHVGDIGTSARTQACGDTWLEARRQQFAKLRAPFVLLPGDNEWSDCARHGADANARLA
                     RWRELFCFEVPRLALERQPRHCENVRWHRDGMAFIGLNVPGGGKPGLGDARMAATLEW
                     LDESLALAEARGATRIFIFMHADPRFERVGTGDAYGRLRAVLATHAAWFAGRLVLVHG
                     DTHVYRDDGPLPGLRRLEPWGAPFVSWLRISIAGGWPRVDAGW"
     gene            complement(15501..>15859)
                     /locus_tag="AC16O9_08105"
     CDS             complement(15501..>15859)
                     /locus_tag="AC16O9_08105"
                     /inference="COORDINATES: protein motif:HMM:NF048679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI/CycH"
                     /protein_id="HIR6516619.1"
                     /translation="AAPPAAALRGSVRIAPALKGKYGAGDTVFIYARAAEGPPMPLAI
                     LRRKASELPLEFALDDSMAMAPGAELSSFPRIVVTARVSRSGQAKPQAGDLQGASTPV
                     PSDASGVEVVIDSVVR"
BASE COUNT         2397 a         5743 c         5494 g         2225 t
ORIGIN      
        1 gccgccctcg agcgcgcgcc agaacaccca gccgatcagg aatacgaggg cgacggagag
       61 ggggacgagg aggtagaggc tttccatgtg ccttccctgg tcagcggtcg cagcgcgttc
      121 aacaccacga tgaaggagct tgccgacatg ccgatggccg cttcccacgg cccgatcccg
      181 cccagcgccg ccgccggcag cgccaccgcg ttgtaggcga gcgcccagcc caggttctgc
      241 cgcaccagac gcagggcgcg gcgcgaaatc gcgatcagct cctcgagtgc gccgagccgg
      301 tccgccgtca gcaccacgtc ggcctgcagc tgcgccgcgt cggcgccggc gcccatggcc
      361 accgagacat cggcgcgcgc gagcaccggc gcatcgttca ggccgtcgcc caccatcgcc
      421 accacgcggc cgtcgcgttg caggcgcgtc acgtactcga gcttgtcctg gggcgtcgcg
      481 ccaccctgcc agcgcgcgat gccgagtcgc cccgcgagcg cgcccacaat cgccggatcg
      541 tcgccactca cgaggtgcac gctcagcccg cgcgccgcca ggccgcgcac gacgtcggcc
      601 gcctcgggcc gcagcgcatc ttcaagggtg aacgcggcaa gccagcggcc ctcttccgcg
      661 agaaacacct gggcgtcgcg cgcctcgggc gccgcgccgg cgatctgctc gcagaagcga
      721 cgcgcaccga tgcgcacccg gcgaccgtcg acgcgcgcct cgacgccctg ccccgcttcg
      781 ttcctcaggc cccctaacgt gggcgcagga cccgtcgccc cgaaggcgcg cgccagcggg
      841 tgggtactgg aagcctcgag cgccccggcg agggcgcggc agtgcgcctc gtccatgtcg
      901 gcgagcagca ccgcccgcgc aaggaccggc cggcccacgg tcagcgtgcc ggtcttgtcc
      961 agcaccacgt cgctcgcgcg ctcgagcgcc tccagcgcgc gtgagcgcgt gagcggtgcg
     1021 ccgcgcgcga ggagctgcgc ccccgcgcgc gtcagcacga tcggcgaggc gagcgcgagc
     1081 gcgcaggggc aggtcgccac cagcactgcg acggcgatcc agacgttgcc cgaaagcgcc
     1141 caagcgaggc ccgccgtggc gatgacgagc gtggtcagat gctgcgcgag gcggtcggcg
     1201 ctctcgacga gcgcagggcg cgtcgccgcc gcgcgctcca cgagacgtcc gatggccgct
     1261 gcctgcgtgt cggcgccggc gcgcgtcacg cgcagcgtga tcggctgctc caggttcacc
     1321 gagccgccga cgaggctcgc gcccgcgcgc ttggcgaccg gacgcgcttc gcccgtgagc
     1381 agcgactcgt cggcgctcga cgcgccttcc tccagaacac cgtcggcggg gatgcgctcg
     1441 ccggccgcca cccgcacccg gtctccggcc acgagcgcat gcgcgggaac gcgctcgccc
     1501 gtgcccccgc gcaggcgcag cgcgaacgac ggcatccagc gcgcgaggcg gtcgagctcg
     1561 cgcccggcct tgtgccgcgc caggagctcg gccgtgcgcg ccgcgagcag caggaacgcg
     1621 agcatggcga tggaatcgaa gtacacctcg ccgctgcccg tgaatgtcgc ccacgcgctc
     1681 gccgcgtagc ccgcggcaag gcccagcgcg atcggcgtgt cgaggctcag gcgccggcgc
     1741 ttcgcgtcct gccaggcgcc gcggaagaac gggccgcagg aaaacagcag caccggcgtg
     1801 gtgatcacta ggctcgccca gcgcagcagc gcggcggtgt ccgcgccgag cgtccccatg
     1861 cccgcgtcca cgtacgccgg ccacgcgtac atcatcacct gcatggcgcc gaagccggcg
     1921 acgaacagcc gccacagcgc gcggcgccgc tcgcgccgcg cgagcgcgtc actgcggcgc
     1981 gggtcgaagg gggtggcgtc gtagccgacg gcgcggatcg cctcgatgat cgttgagagc
     2041 cgagtccgct gcgggttcca ggcgatctgc gcacgctgcg tcgcgtagtt gacgtcggcg
     2101 cgcagcacgc cgggcacgcg gcgcagggtc tgctcgttca gccacaggca ggcggcgcag
     2161 cggatgcgat cgaggatcag cgtcgcttcg cggacgttct ctccggccgc ggcgacgaac
     2221 tcgcgctgcg cggccgggtc gtcatagacc gccgggggcg gcagcaccac cgggcgcgcg
     2281 cccgcgctgc tgcgcgctgc gtagtatctt tcgaagcccg cgccggcgat ggcccgcgcc
     2341 accgcctcgc accccgcgca gcagaaggcg cgctccgcgc cgagcacagg ggcgagatgc
     2401 ctgcccggct cggcaacggg caggccgcag tgaaagcagg cgctcaggtg acgctgacgc
     2461 gcttgccgcc gccgttcgcc ttcttcggca gcagaagatt gaggacaccg tccttgtatt
     2521 cggccttggc ggccttctcg tcgacctcga ccggaagcgc gaagctgcgg ctcaccatgc
     2581 cgtagctgcg ctcgctgtac aggaccttct cgtccttctt ctcttccttc tgctgccggg
     2641 tctcggcgcg caccgacacc acgttgccgt cgatgtcgac ctggatgtcc tccttcttcg
     2701 cgccggggat gtcggcgtgc acggtgtagc ccttgtcgtc ctccttcacg tccagcttga
     2761 tctgcatttc ggggccggtc gggaaggcga gcggcttcac ccagaagccc ttgccgaatt
     2821 cgttgaacag ctcgtcgaac gggttgtagc gggttacgtt gcccataaaa tcctccgcgt
     2881 gttgcactgc caataaaatg ctaggcgagt gtgcgacgcc cgtattgacc ttcgtcaaac
     2941 gcctctgctg gctcgagatc taggctggcg ggatcccgcg gaattgtcgg cccgagagac
     3001 tgtcacacca agaaggaggc cacccatgtt caagcacatc ctgctccccg tcgacggatc
     3061 gaagctcgcc ctgaagggtg ccaaagccgg cgtgcggctc gcgaaggcgc tgcgcgcccg
     3121 cgttaccgcc gtgtacgtga tcccgccttt catgcccgtc gcctacggcg acgcggcggt
     3181 gtacgtgccc gcgctttcgc cgcttgaata caggaaggcg accgagaagg gggcgcgcaa
     3241 ggcgctcgcg gcggtcgagg ccgacgcgaa gaaagcgcgc gtgcgctgca agacccgctt
     3301 cctgaccgcg gcgcaggtct acgacggcat cctgcgcacg gcgcgcggcg cgaagtgcga
     3361 cgccatcgcc atggcctcgc acggccgcgg cgggctcgcc ggagtgctcc tcggcagcga
     3421 gaccacgcgc gtactggcgc actccaaggt gccggtgctg gtcctgcgct agcgcgtaaa
     3481 cttaattagg gacagacctc attttttcgc tatgcgaaaa aatgaggtct gtccctattt
     3541 tccggcggac ttggcgacga agcgggcgac ggcctcgatc tgctcggggg tcagcttgtc
     3601 gctgaacggc ggcatgatgc cgacgccctg acgtacagcg gccaaggtct gctccaggtc
     3661 gggcttgagc tcgtcgagcg aagggccaac cgtccccgcg gcgccggccg ccttcagcgt
     3721 gtggcacagc tggcacggcg gctgcgctag ctccgtgaag accttgcgcc ccagctcctc
     3781 gccgtgcgcg gcgcaggaca gggcgaacgc gagccccgcc gccgccgctc tcacgccacg
     3841 gtcactttga ccgcgtggtc gcgccagccg ttgtggccgt agccgcgctc gttctccacg
     3901 cgcagctcgg gctgcacttc gccgtcgtag gcgtgcgcgc ggctggtgat ggtgtaggtg
     3961 cccgggctga ggcgcacggc gagcacgaac tggcgccacg cgtgcggccc gagcagcggg
     4021 ccgaagaatt cggccgctcg ccagctcgca ccgccgtcga gcgacacttc gacgcccttc
     4081 accgcgctcg tgccgctcca cgccacgccg gtgatctgca ccatgccggc gcgcacggtc
     4141 tggccgtcct cgccggacgg gttcgtgacg aaggacttca ggttcatgtc ccacatcgtc
     4201 ggctggcccg gcgcgccctt ctcgccgatc ggccgcacac ggtagctcga cgactggatg
     4261 cgcgcatcgg tctggtccgc ggtgaactcg acgcgcttca cgtactttac gttattcacg
     4321 ccgtagtagc cgggaatgat catgcgcacc gggccgccgt tcgcgaccgg cagcggcgcg
     4381 ccattcagct cccaggcgag gattgcgcgc tccagcatgt tccagggcac cgagcgctcg
     4441 acgagtgcgc gtttcggatc gacgccggcc ggcagggtct cgccgccggt gctcgtcatg
     4501 tagcgcatgc cctggcgcac gccgcccacc gcggccacca cggccttcat cggcaccccg
     4561 ctccacagcg agcagccggc cgcccccgtc ttccactgcg tgccgcccac cttgtggccg
     4621 aagaacgcgc gcccgttgcc cgagcactgc agcaccatcg gcaccgcctc gacgccgagc
     4681 gccttcagct cgccgagcgt gaagctgcgc gggttggtca cgccctggat ctcgacctgc
     4741 caggcgtcgg cgttcttcac cgcggcctcg tccggcgggg tgaggttgtt gcgcacgaac
     4801 aggacctcgt ttgccgtgat gacgctcgtg ccctgcgcgc cgcgcaccgt ttccatggtg
     4861 ttggaggaat gcacgatgag gttcgcttcg tccttccacg cggcgtagcc gggcagcggc
     4921 ttgcccttgg cgagggccga cggcgcgagg cccgcgagtc cggcggcggc ggcgccgccc
     4981 gcgctggtca tgaggaattt gcgacggttc ctgtcgaggc tcgctgtcat ggcatctccc
     5041 ggggtagcac tgttaccgaa ttctccacac atttctcagc cgcgcaagca ccgccaccgc
     5101 ttttcgcgca aagagcggcg cgctattcga ggtcggtata cagcaggcgc ggctcggccg
     5161 gcacgctctc cagcgtgaaa tagacgcgtc cgatctcgcg cccgtcctcg gtctcgacct
     5221 gcaccttcca gcgcccgggc tggtagttgg ccttgtagcc gtagccgcgg aagccctcct
     5281 cgcgtccgcc gacgatgtcg atcgggatcg agtcctgcag cagccagccg cgcccgcccg
     5341 gctcccagta ccagcgcatg cgcacgcggt cggcgaatcg cgcgggcgag aacacgcgga
     5401 agaacacgta cacccggtcg ccgggctgag cgcggaagtc ctggtcgccg tggtgccaga
     5461 agcgccacca cggccgctcg tgcgccagcg cgtagccccg ctcggtgcgc tcgacgccgt
     5521 gatagacgcc gatgaagggg atcgacagcg gcgcaggcgg gatcacacgc agcaggtagg
     5581 cggcgaggaa cgcgagcagc accgcacaga gcggcagcag catctgccgg cgcacctgcc
     5641 cgaacagcgc gcgacggcgc cgccgcaccc accacgcaac gcctgcgagc ggcatcacgc
     5701 ccgccagcat ggaagcgacg aacacccagg tgccgatcga gccgagcgcg atcggcacca
     5761 cgatggcggc gaaggacagg taacacaagc cgagcagcgc gaacttgaaa tgcaggccga
     5821 tcgccttgac gcgccgcgac tcgttcgcca ccagcagcgc cagcaggaag gcgagaaagc
     5881 cgaacgagac caggagcgag ctgctcttga agaagaagat cgcatacagg ttcatcagcg
     5941 cgccgagcgc gaagtggatc gccgccgagc ggtactcgta gtaccagtgc acgatgcgcg
     6001 gctggccggc gagctccacc ggcggcgcgc cctgctcgag cagcatgtgc agcagcaccg
     6061 cggtgaccgc ggccaggtag agcgtttgct gcgcgatcgt gacccaggaa tcgacgcgcc
     6121 cgaccgtaaa gatgtcgaag atgaagccgc cggcgaagaa ggcgaccacc acctcgcgct
     6181 cgttctgcgc gaagcgcagc cgcaggcgct cgaacgccgt cgccggggcc gccggtacct
     6241 cgggctgcgt catcggaacg gcgattgtac cgatcgtacg gtcgcggccc gcgccagctt
     6301 gtagactgcg gtcatgcaag cccgctccgc gctcgccgtg ctgctgctct cgctggggct
     6361 cctcgccgtc cccgcgtcgt cccccgccca gccggataag cgcgtggtcc acgtcgcacc
     6421 gatcgaaggc gtcatcgacc tgggcctcgc gcccttcgtg cggcgcgcga tcgacgaggc
     6481 cgcgcaggcc ggcgcggcgg cgctggtgct cgaagtcaac acgttcggcg ggcgcgtgga
     6541 cgccgcggtg cagatccgcg atgcgctgct caacagcccg gtcaagacca tcgccttcgt
     6601 caacaagcgc gccatctccg ccggcgcgct gatcagcctc gccgccgagc acctcgtcat
     6661 gtccggcggc agcaccatcg gcgcggcgac gccggtgcag gcaggagcgc cgggcgccgc
     6721 cgcccagccg gtgcaggaaa agaccgtgtc ctacgtgcgc aaggagttcc gcgccaccgc
     6781 cgagagccgc aagcgcccgc cgcggctcgc cgaggcgatg gtggacgccg acgtcgagat
     6841 ccgcggcgtg atcgccaaag gcaagctgct cacgctgacg accgacgagg cgctcaagca
     6901 caagctcgcc gattttcgcg ccgataccct ggaaagcgcg ctcgaaaagc tggggctcgg
     6961 cggcgccgag ctgcggcgcg ccgcgcccaa ctgggcagag aacgtggtgc gcttcctgac
     7021 ccacccggtg gtgagctcgc tgctcatcag catcgccatg atcggcattc tgatcgagct
     7081 gcgcacgccc ggcttcggca ttcccggcgc ggcgggcgtg acaagcctgg cgctattcct
     7141 ctggggccac tggctggtgc agctcgccgg gtgggaagag ctgctgctcg cgctcggcgg
     7201 cgtggtgctg ctcgcgctgg agctgtttgt cattccggga ttcggcatcg ccgggatact
     7261 cggcatcctc gcgctgctcg cagcgctggt gctcagcatg ctcggcacgg gcgacacgcc
     7321 acaattcatg atgctggccg ccacgcgcgt cgtgttcgcg ctgctcgccg ccctggtggc
     7381 gagcctggtg ctgctgcgtt acctgccgcg cctgcccttc gggcggcgct tcgtcttgcg
     7441 caccggcctg gcgtcggcgc agggatatgc gtcagcgccg gaaagcgacc gtcagtggct
     7501 gggcaggaaa ggccgcgcat cgtccccgct gcacccggcc ggcatcgccg aaatcgacgg
     7561 ccagcgcctg gacgtggtct ccgacgggga actcgtcgag gcgggcgagt tcatagaagt
     7621 gatccgcgtg gacagtaacc gcgtcgtcgt cagacgaatc gtcaacccca atgcaaagga
     7681 gtgacccatg acagggctgg agacaagcct caccggaggt ctaggattgc tcatcctgct
     7741 ggtggcaggc ctggcactgc tgctgtacct ggtgccggtg ccgctgtgga tcgcggcatg
     7801 ggcctcgggc gcctacgtgg ggctgttcac cctgatcgcc atgcggctgc gccgcgtgcc
     7861 gcccagcacc atgatcacgg cgcgcatcag ctcggtgaag gccggcatcg acgtctcgct
     7921 gaacgacctc gaggcccact ttctcgcggg cggcaatgtc gtgcgggtcg tcaatgccat
     7981 gatctcggcc gacaaggcca acatccccct gcccttcaag cgcgccgcgg cgatcgacct
     8041 cgcgggacgc aacgtgctgg aggcggtgca gatgtcagtg ctgcccaagg tgatcgagac
     8101 accgcgcatc gcggcggtcg ccaaggacgg catccagctg atcgtcgtct cccgcgtcac
     8161 ggtgcgcacc aatatcgacc gcctggtggg cggtgcgggc gaagagacga tcatcgcgcg
     8221 cgtcggcgag ggcatggtga gcaccatcgg ctcggctacc aatcacaaga gcgtgctgga
     8281 gaaccccgac catatctcca agcacatcct ctccaaggga ctggacgcag gcaccgcgta
     8341 cgagatcctg tccatcgaca tcgccgacgt cgacgtcggc gcgaacatcg gcgcgaagct
     8401 gcagatcgac caggccgacg ccgacaagca gatcgcgcag gccaaggccg aggagcgccg
     8461 cgccatggcg gtggcgctgg aacaggagat gcgcgcgcgc gtcgtggaag ccgaggccga
     8521 ggtgccgcgc gccatggcgg aggccttccg ccagggcaat ctcggcatca tggactacta
     8581 ccgcatgaag aacctgcagg ccgataccga catgcgcggc gtgatcgcgg gcaccggcga
     8641 tgatcccgcc ggcgcaccga agaagtagcc ggggccgcga tgccgaaatc cggtaccgaa
     8701 ctgctgtttc tcgggctggt catcgccggc ttcatgctgt tcaactacgt ggcgcagcag
     8761 ctcgcgaaga aagcccgcgc gcagcaggag gcgacggagg cggaaccggc cgcagaggcg
     8821 ccggcgcccg cacctgagga cgagccattg caggacatct gggggcgcgc gcccgcgaaa
     8881 cctccggccg cacccgaagt tgttgcacgc gccgtaccgg agccgcaggc cgccccgccg
     8941 ctgccatcgc ccgggcaccc gggtgtccgg ctgtttcgga cgcggaagga tctgcaacgc
     9001 gccatcgtcc tcatgacggt gctcggaccc tgccgcgcgc tcgagccgca cgaacgccgc
     9061 tagccagcaa cgtccgccta gcgcagcagc cgtcgcacga aggcgccgat cacccaggcg
     9121 cgcaccagcc aggcgcgcag ccgcggcgag ccgctggcga gcgccaccgc cacgccgagc
     9181 ccgagcacca gcggctcggc gacggcttgg cgcacgccgc acgccgcggc ggcgacctgc
     9241 gcccgctgcg ccgccgagcg cgagaccagc gcctcgcgcc gccgcgcgag cgtcgccagc
     9301 accgccctca ttcgcgcggc tcgagcgctt ttgcgtcgcg ctcgagctcg ccgaggctcg
     9361 cggcgaacgg gcgcgcgcgc gcttcgcgcc gcacgcgcca cgcgatcagc gccgctgcgg
     9421 cgagaaacgc caacgccagg cccgcggcca ccgcgacgcg atgctcgggc gccaccgaca
     9481 tcaccagcgc caccgcgccg aagccgagcg caagcgcagc gagcaggatc gccgccaccg
     9541 cgcccaccat gatgagcacc aggcgcgcga gcagctcctg catctcgacg ctgagcagct
     9601 cgagccgcgt gcgcgcgagc ccgagcagcg tggcaagcag gccttgcacc gactgcagca
     9661 ggcccacggc tacttccgcc cgaggagcag cccgagcacc aggccgacgc ccgcggcaat
     9721 gccgagcgcc gcccaggggt tgcggcgcac ctggtcctcg atctcgcgcg cggcctcgcg
     9781 cgcgctgtcg cccgcggccg cacccgcctc gcccaggcgc tcgcccgact cggcggcggc
     9841 ggccgcgagc aagccctcgg cgtcgcgcac cacggcgcgc aggtcggcca tcagcttttc
     9901 ggttgtggca ggatccatcg caaagcctcc tccgttaagc ggcgattgta tattccgtgg
     9961 atggccggcc tgtccgaacg gcattacctc accccgctgt tcgagccggc cgcggtcgcg
    10021 gtgatcggcg ccaccgagcg cgcgggcgct gtcggcgcgg tgctgacgcg caacctgctc
    10081 gaggcaaggt atcaaggcac gctgtttgcg gtgaatccca agcatcgctc ggtgcacggg
    10141 gtgccctgct tcggcgccat cggcaaggta ccgggacgcg tcgacctggc ggtgatcgcg
    10201 acgccggcgg ccaccgtgcc cgacatcatc gagcaatgcg gcaaggccgg cgtgcgcgcg
    10261 gcagtggtga tcagcgccgg attctcggaa accgggcccg ccggcgcgaa gctggagcgg
    10321 gccctgctgg agaacgcgcg gcgccatcac ttgcggctcg tcggccccaa ctgtcttggc
    10381 ctgatgcgcc ctgcgctcgg gctgaacgcc accttcgcgc gcggcgcggc gctgcccggc
    10441 tcgctcggcc tggtggcgca gtcgggcgcg gtgtgcaccg cgatgctcga ctgggcgcgc
    10501 ccgaacggca tcggcttttc gagcgtggtg tcgctcggcg gctccaccga cgtggatttc
    10561 ggcgagatca tcgactacct gatcggggat ccggcgaccg agcacattct gctgtacatc
    10621 gaaggcgtgc gcagcgcgcg gcgcttcgtc tcggcgctgc gcgccgcggc gcgcgtgaag
    10681 cccgtcatcg tgcaaaaggt cgggcgccac ccggcgggct cgcgcgccgc agtgtcgcat
    10741 accggcgcca tcgtcgggct ggacgacgtg ttcgacgcgg tggtgaagcg cgcgggcgtg
    10801 gtgcgcgtga agacgatcgg ccagatggtc gccgcggcgc aagcgctcgc ggcgcacgtg
    10861 cgcccgcgcg gcgaccggct cgccgtggtc accaacggcg gcggcccggg cgtgatggcc
    10921 gccgaccgcg ccgccgacct cggcattccg ctcgccgagc tcgcgcccgc caccctcgag
    10981 gcgctgaagc cggcgctgcc cgcgaactgg tcgcacggca acccgctcga cctgatcggc
    11041 gacgccgatg ccaagcgcta ccgcgccgcg gtcagcgcat gcctcggcga cgacggcgtc
    11101 gacggcgtgc tcgccatcct tacgccgcag gcgatgaccg agcccgatgc ggccgccgcg
    11161 gcgctgatcg aggcggcgcg cggctcgtcg aagccggtgc tggcgtgctg gatgggcgac
    11221 gagcagaccg cggcggcgcg ccggcggctc gccgcagcgc gcatccccgt gttccgcacg
    11281 cccgatccgg ccgtggacat gttcggccac gtgtcggcgt tctaccgcaa ccagcgcgcc
    11341 ctgctgcaga cgccgggacc ggtggccgcg ccggacgcgc ccgatctcgc caccgcgcgc
    11401 gcgatcgtgg acagcgcgct tgccgcgcgc cgcagcgtgc tctcggaaat ggaatcgaag
    11461 gcgctgctcg cggcattcgc catccccatc gcccgcaccg tcgtcacgcg ctcggcgcac
    11521 gaggcgatgc tggtcgcggg cgagatcggc tttccggtgg cgatgaagat cgactcgccc
    11581 gagatcacgc acaagagcga cgtgaacggg gtgcgcctca acctcggcaa cgcgcaggcg
    11641 gtgcgcgcga cctgggagga gatcgtcgcc gaggccaagc gcctgcggcc cgaggccaag
    11701 gtaaacggcg tcaccgtcga gccgatggtg cgccgcgcgc acggccgcga gctgatggtc
    11761 ggggtcatcc gcgacccggt gttcgggccg gcgatcgtgt tcggcaccgg cggcacggcg
    11821 gtggaggtgc accgcgaccg cgccgtggcg ctgccgccgc tcaacgcctt cctcatcgcc
    11881 gacatgatcc gcggcacgcg cgtggcgaag ctgctcgggc cgtttcgccg catgccgccg
    11941 gtggacatgg cggcgctgga gagcatcttg ctgcgcgtct cggagatggt gtgcgagctg
    12001 cccgagctcg ccgagctcga catcaacccg ctgatcgcgg atgcgtcggg cgccatcgcc
    12061 gtcgacgccc gggccgtgct gcagcccggc gcgccgcagc gcgaccgcta cggccacatg
    12121 gcgatccatc cctatccggc acacctcgtc ggcacctggc ggcccgccga ggggccggaa
    12181 gtcacgctgc ggccgatccg cccggaggac gcgcagatgg agcaggcgtt cgtgaagagc
    12241 ctgtcggccg agacgcgcta cttccgcttc atggacacgc tgcgcgagct cacgccgcag
    12301 atgctggtgc gcttcacgca gatcgactac gaccgcgaga tggcgttcgt cgccacggtg
    12361 cccgaggacg gccgcgaggc ggaaatcggc gtcacgcgct acgtcgccaa tcccgacggc
    12421 gagagctgcg agttcgcggt ggtgatcgcc gacggctggc agcgccgcgg cctcgggcgg
    12481 cgcatgatgg agcagctgat cgacgtggcg cgcgcgcgcg ggctgcgcgc catggtcggc
    12541 catgtgctcg ccgagaaccg cggcatgctg gcgctgtgcc agaagctggg cttcggcgtc
    12601 gccgacagcg ccgaaggtcc gatcgtgaag cgcgtgaggc tcgcgctgac ggagcgctga
    12661 gccgctacag gccgaggttg cgcgccacgc gcagggcctg ggcgctgcgc gcgtcttccc
    12721 tgggcaccac cgcggcgacc gcagcccagt cggcaccctg caacaactgg gcggcgcgcg
    12781 gcatgatcgt gctttcctcg gtcgccatgt ggctgcggta gtacgcaaag aaagtggccg
    12841 ccgcggcttc gagatcggcg cgcggcgcaa agccgccgtt ggcggcgttc tccaggcgct
    12901 cgagcagcgc ttcgcccgcg tacgcgatga cgcggtgctc ctgcagcagc cggttgaccg
    12961 gcaggcgcag ggccggatcg cgctcgacca tgcgctcgaa ggccacgtcc tcgcgcgggt
    13021 ggtgcacgcc gtcggcatag tggcgcagcc acagcacgat gtcgcgcatc agctcgtaat
    13081 tgggctgctc gcccgagtgg aacagcgtga cttccttctc cagcatgtcg agcagccggc
    13141 cgaagcgcac gtgatcggcg tgccaggcgg caatcgggtc aaccatgatt cacatcagac
    13201 aacgcgccgc ggcgcggcgc ttgatctgga tcaagcgccg cgcgcgcccg attgaggcgc
    13261 gcaatggcgc gcatcgcccg attcgattgg agtcccgccc ctgcccgacg tatccttgag
    13321 cgattcgctc aaggaagaac ccatgaccgt ccgcgaaggc agtctcgacg cccccacccg
    13381 ccaccctctc gactggcgca atcccgagtt ctacgacgag cacaagctcg acgccgagct
    13441 cgagcgggtc tacgacatct gccatgggtg ccggcgctgc gtgaacctgt gcaccgcgtt
    13501 cccgacgctg tttgacctga tcgacgaatc caagacgatg gagatcgacg gcgtcgccaa
    13561 gagcgatttc tggaaggtcg tcgaccagtg ctacctgtgc gacatgtgct acatgaccaa
    13621 gtgcccgtac gtgccgccgc atccctggaa cgtggacttc ccgcacctga tgctgcgcgc
    13681 caaggcggtc aagttccggc gccatggcgc gggactgcgc gaccggctgc tgtcctcgac
    13741 cgacgcgctc ggcaagcttg cggcgatccc ggtggtggcg caaacggtga acgccatgaa
    13801 ccggctgccg gcggcgcgcg gcgtgatgga gaagatgctc ggcgtgcacc gcgagcgcaa
    13861 gcttcccgat tacgccagcc gcaagttccg ctcgacggct gcgccgagcc gcgcccgcgc
    13921 cgtgaaggac ggcaagaaca cgcccggcaa ggtggcgatc ttctcgacct gctacgtgaa
    13981 ctacaacgag cccggcatgg gccacgacct gctcgcgctg ctcgagcaca acgagatccc
    14041 ctacgtgctg gtcgaaaaag aggcctgctg cggcatgccc aagctcgagc tcggcgacct
    14101 cgaggcggtg gcgaggctga aggacctgaa catcccgccg ctcgcgcgcc tggcgcgcga
    14161 gggctacgcc atcctcgccc cggtgccttc ctgcgcgctc atgttcaagc aggagctgcc
    14221 gctcatgttc ccggacgagg ccgaggtgaa gcgcgtgcag gaggcgatgt tcgacccgtt
    14281 cgagtacttc gtgacgcgcg cgaaggacgg cctgctgaag aaggatttca aggaaaagct
    14341 cggcaaggtc tcctaccacg tgccgtgcca ctcgcgcgtg cagaacatgg gccagaagac
    14401 gcgcgagatg ctggagatgg tgccgggcac ggagctcacg acggtggagc gctgcgcggg
    14461 ccacgacggc acctggggcg tgaagcgcga gtacttcgac aactcgatga agatcggccg
    14521 cccggtgttc cggcagatgg ccgagcccgg ccccgactac gtcagctccg actgcccgat
    14581 cgccgcgcgc cacatcctgc agggcatggg cgatgaagcg ggcaaggccg cgaaagccca
    14641 tcccctcacg ctgctgcgca aggcttacgg catctaggct cgtcctcgcg gcgctcctcg
    14701 ccggctgcgt gcagctcacg ccgccgcccg gggcgccgtt cgccttcggc gtgctcggcg
    14761 acacgcccta ttcggcgccc caagcgcgcg cgctcgacgg cctgatcgag cgcatcaacg
    14821 cgcaagacct cgctttcgtc gtgcacgtgg gggacatcgg caccagcgcg cggacgcagg
    14881 cctgcggcga cacctggctc gaagcgcgcc ggcagcagtt cgcgaagctg cgcgcgccct
    14941 tcgtgctgct ccccggcgac aacgagtgga gcgactgcgc gaggcacggc gcagatgcga
    15001 acgcgcgcct tgcgcgctgg cgcgagctgt tctgcttcga ggttccgcgc ctcgcgctcg
    15061 agcgccagcc gcggcattgc gagaacgtgc gctggcaccg ggacggcatg gcgttcatcg
    15121 gcctgaacgt gccagggggc gggaagccgg gcctcggcga cgcgcgcatg gcggcgacgc
    15181 tcgaatggct cgacgagagc ctcgcgctcg ccgaagcgcg cggcgcaacc cgcatcttca
    15241 tctttatgca tgccgatccg cgcttcgagc gcgtcggcac gggcgatgcc tacggccggc
    15301 tgcgcgcggt actcgcgacc catgccgcgt ggttcgccgg ccgcctcgtc ctcgtgcatg
    15361 gggacacgca cgtgtaccga gacgacgggc ccttgccggg actgcgccgc ctcgagccgt
    15421 ggggcgcgcc gttcgtgtcc tggctgcgca tctcgatcgc cggcggctgg ccgcgcgtcg
    15481 acgccggctg gtagccggct tcagcgcacc acgctgtcga tgaccacctc gacgccgctc
    15541 gcgtcgctcg gcaccggcgt gctcgcgccc tgcaggtcgc cggcctgcgg cttcgcctgg
    15601 cccgagcgcg acacgcgcgc cgtcaccacg atgcgcggga acgacgacaa ctcggcgccg
    15661 ggcgccatcg ccatcgagtc gtcgagcgcg aactcgagcg gcagctcgct cgccttgcgc
    15721 cgcaggatgg cgaggggcat cggcgggccc tccgccgcgc gcgcgtagat gaacaccgtg
    15781 tccccggcgc cgtactttcc cttgagcgcg ggcgcgatgc gtaccgagcc gcgcaaggcc
    15841 gccgccggcg gcgcggcgg
//
LOCUS       DBNHKE010000127        50743 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_338671, whole genome shotgun sequence.
ACCESSION   DBNHKE010000127 DBNHKE010000000
VERSION     DBNHKE010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 50743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 50743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50743
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_338671"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="AC16O9_08110"
     CDS             complement(<1..125)
                     /locus_tag="AC16O9_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6516620.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTHILAKKYGG"
     gene            complement(172..246)
                     /locus_tag="AC16O9_08115"
     tRNA            complement(172..246)
                     /locus_tag="AC16O9_08115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(212..214),aa:Thr,seq:ggt)
     gene            complement(260..333)
                     /locus_tag="AC16O9_08120"
     tRNA            complement(260..333)
                     /locus_tag="AC16O9_08120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Gly,seq:tcc)
     gene            complement(390..476)
                     /locus_tag="AC16O9_08125"
     tRNA            complement(390..476)
                     /locus_tag="AC16O9_08125"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Tyr,seq:gta)
     gene            complement(615..1430)
                     /locus_tag="AC16O9_08130"
     CDS             complement(615..1430)
                     /locus_tag="AC16O9_08130"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HIR6516621.1"
                     /translation="MRIALLGAGTIARLVLQHLRCGDLPGMQVVGIGGRTSASRGVAL
                     AREYALPYVVGGKALLALQPQVVVEAASHDAVRENLVPFLEAGVGVVVLSAGALADDR
                     LRDAAEDAARRGGGQLYVPSGGIGGLDALKAACVAGVDEVSIRVAKPPRAWQGIPFVE
                     ALGIDLDRVTQPRTLFKGAARAGVPHFPQNVNIAAVLSLAGIGFDRTELEVVADPTLT
                     HNTHTIRVAGKSGRFTVVLENVPSPDNPKTAWLACYSALAALRALAGAVRYGT"
     gene            1505..2209
                     /locus_tag="AC16O9_08135"
     CDS             1505..2209
                     /locus_tag="AC16O9_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6516622.1"
                     /translation="MRISILEDDPDQLALLKRWLADDGHDVHGYLSGRDAMKHAGRES
                     FDLFVIDWQVPDVSGVDVLMWLRTNVSKQVPVLFVTVRDAEQDIVFALENGADDYMIK
                     PVRRQETIARVHALLRRAYPRVEEKLLSFPPFEIDTAKSEVRRNGERIELTPKEYELT
                     VVLFRNLGRLLSRGHLQEAVWGRSGDLITRTVDTHVSQVRKKLDLRAETGFRVVPIYN
                     YGYRLEQVGEGNSSVA"
     gene            complement(2211..3344)
                     /locus_tag="AC16O9_08140"
     CDS             complement(2211..3344)
                     /locus_tag="AC16O9_08140"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516623.1"
                     /translation="MSIINKMLQDLDRRHARSAPPGQSLHPEVRAVRGADGSGEWFWR
                     IVAGLMLVAAAWTLWVVYQLQPRPIATELAARAEDVSRQRTVSAPAPQDTAAVMVLAA
                     APAADAQPVPLEAVAAKPAPEKLAAPIEMLRLALTIDTPLAPRPQRPAAPTPSEKQTP
                     RVAAAPKPRAEPARVAPAHIEKRERDRSPADRAENHFRRAAQMLERARVADAEDALLA
                     ALDADAAHQAARQTLVALHIEQGRIDDARRHLQEGLAFNSGYAPFAVALARIHVDRGD
                     FSAALSVLEQAAVAGRGNPDFHALHGAVLQQLRRHGAAADAYRLALASAPQSGAAWVG
                     LAISLEAQGHLPEAAEAYRRGVASGTLTGDVRAYADQRARQLK"
     gene            complement(3354..4217)
                     /locus_tag="AC16O9_08145"
     CDS             complement(3354..4217)
                     /locus_tag="AC16O9_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR6516624.1"
                     /translation="MNYFRHFGLREAPFGITPDTSFFYACRSTQEALNTLLIALGNGE
                     GFIKITGEVGTGKTLLCRKFLGSLDSNWASAYIPNPNLEPRALVLAIAEELAVPVEPD
                     VDQHHLLRGITRVLLDHARHARRVVLCMDESQGMPLETLETLRLLTNLETEKRKLLQV
                     VLFGQPELDRKLASESIRQLRQRITFQYALRGLHRDEIGAYIEHRLAIAGLVGGPLFD
                     AGALRALHRASRGVPRLVNILAHKALLLVFGEGGRCASRRHVHAAAVDTPSAGPVSAW
                     WWLLRTSRAAD"
     gene            complement(4214..5932)
                     /locus_tag="AC16O9_08150"
     CDS             complement(4214..5932)
                     /locus_tag="AC16O9_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR6516625.1"
                     /translation="MKYSMVCLGVLLMAACATPPPRPTSVDPAITGEVDKAIASPGPQ
                     ETRLPQQALLPPLRMEMPSVEGRPIDSRFDLSVSNAPAAQVFMAIASGTRYSMLLNPG
                     ITGLITVNLKDVTVREALDSLRELYGHDYRIEGNRIFVQAAGLQTKIYRVNYLLGQRR
                     GTSDLRVQSGSLADSSAPSGPPTPASTASPVPSTGSGRAMDSTRVTTSIFNDFWGDLR
                     TALTAIVGTGDGRSVVVTPQSGVVLVRAFPTELRSVESYLRETRLVVERQVMLEAKVI
                     EVTLAEGFQSGINWGLFRTHDSPRGAIGQIGNNTTLGSNISSATLSSQGITVNPAAGT
                     LASAATLAGNGGAVFGLAFQTSNFAALLQFLDSQGTTQVLSSPRIATLNNQKAVLKVG
                     TDEFFVTNVATVTTTTGTTTQQTPTVTVAPFFSGIMLDVTPQIDQFNNITLHIHPSIS
                     EVTESRRVVDLGGTIPSITLPLAKSAVNESDTVVRVTDGNIVAIGGLMSVDVRDGRTG
                     IPGVADSNVLLRNSDRQARKREIVILLKPTVIQNDASWGQDLRETRERLERFIPRRPP
                     QEAGSR"
     gene            complement(5929..6144)
                     /locus_tag="AC16O9_08155"
     CDS             complement(5929..6144)
                     /locus_tag="AC16O9_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516626.1"
                     /translation="MISPTRRIAVISGSTVGLGEKFGGATVVAISEGAVMLRYADRRE
                     TLHLIPGVVKRERRADAETQLLKGNSR"
     gene            complement(6297..6944)
                     /gene="gspM"
                     /locus_tag="AC16O9_08160"
     CDS             complement(6297..6944)
                     /gene="gspM"
                     /locus_tag="AC16O9_08160"
                     /inference="COORDINATES: protein motif:HMM:NF016493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HIR6516627.1"
                     /translation="MSALLKRYAERIDNATLRERVLVFLAAALILVLVINGALIKPLQ
                     DSQRRLSAQISQNERELRTIQGEVQRLARARDGDPDARSRERVAVLRAEIAALEGQIA
                     EEQRRFTTPQRMRGVLEEMLQRDKRLRLIDLKSLPVTELAATQGQAGRRVFRHGVELT
                     LAGSYLDLYAYLAALESLPTQLYWGRAEMSVSEYPVATLKLTVYTLSFDQAWLVV"
     gene            complement(6944..7600)
                     /locus_tag="AC16O9_08165"
     CDS             complement(6944..7600)
                     /locus_tag="AC16O9_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516628.1"
                     /translation="MSQQINLFNPLFMRKEKYFSARTMLQSLGLIVLGLVVLYAYALW
                     QSVDLKRLAAGYGAQLAAQREQFVALSGQGRSKLLEAEVARLEASLRAQRGVLDVMQG
                     GGLGNTDGFSRFFAAFARHPMRGVWLTGFSVGDNGNVLNIHGRALHPDLLPEYLKSLN
                     RDDVMRGRLVTELKLVARDESPSRGGSAPGGPLRFVEFDLSAPIRLVDAGGPATKGAR
                     "
     gene            complement(7608..8549)
                     /locus_tag="AC16O9_08170"
     CDS             complement(7608..8549)
                     /locus_tag="AC16O9_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HIR6516629.1"
                     /translation="MKWPFSRTTREPGWLAVSLQPGELRFVHGNVALGGTATVDVCGT
                     RVFEGEFDAAQNAAREHGLGRYQCSTLLVPGEYQMLLVEAPNVPQAELKTAIRWRIKD
                     LLDYHVNDATVDVLAVPPNPAAGGRAQSMYAVAARNDLIQACIGRFESARIPLSVIEI
                     PETSQRNLAALFEDEYRGLAMLYIGQGEGLLTINFRRELMLARRLDVGMQEMASSSGA
                     AREELFQRVLLELQRTFDHFDRQFAYVPVAKLMLAPDPEETGLLEYLAGNLDVPVERV
                     RLSAAIDFGARAELEPKEEWRFFHLIGAALRHESKEL"
     gene            complement(8663..9085)
                     /locus_tag="AC16O9_08175"
     CDS             complement(8663..9085)
                     /locus_tag="AC16O9_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshP"
                     /protein_id="HIR6516630.1"
                     /translation="MKSFLRRSRGFALVLALFLIVSLSAIGAYLLTVSNVQVESGLMD
                     EQGARAYQSARAGLEWGAYRVLRNATCPAGTTTIALAANLAGFYAEVTCTSFGAENEG
                     GTPVTTYRIVSTGCNATPCASGAGAVYVERQLQLTLAR"
     gene            complement(9082..9975)
                     /locus_tag="AC16O9_08180"
     CDS             complement(9082..9975)
                     /locus_tag="AC16O9_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR6516631.1"
                     /translation="MRPIRPERGVTLIELVAVIVIVGIIVAAVSFFLTPLRQGTDIAA
                     RAELTDIANNALQRMGRDVKLALPNSVRLASAGTSRFVEFIAVRTAGRYRADGGGPAG
                     GTNCPADDAALPAPDNDQLSFDLPGGDQCFKTLGKLPGAFVAGDQLVLNNYGFPDQNA
                     YQTAGTLNRVPITAVDTSEGARDRVAFAPTTFVRALHDSPGKRFFVISGPVTYECDLA
                     AGRIYRHSAYALAAAQPTSFGGTPALVAEHVTGCVFEYAPNVSAQLGLLTLRLTLQRQ
                     LFGGTPETVALYHAVHVNNAP"
     gene            complement(9956..10504)
                     /locus_tag="AC16O9_08185"
     CDS             complement(9956..10504)
                     /locus_tag="AC16O9_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR6516632.1"
                     /translation="MFSSRQHGLSLIEVVIFIVVLGIGIAGLAVLYNRLTLSSVDPLV
                     RKQAVAIASSLMEEIQLRPFTFCDPDDPAVFTATGVGDCATPEGIGVEGGETRYGPTF
                     FDNVSDYAGPPDMLGSIQDITGATVLTGYSAQVRIVAAGGDFPAAIPADEALRITITV
                     TGPANTQVVLQGYRLRYAPNSP"
     gene            complement(10494..10910)
                     /locus_tag="AC16O9_08190"
     CDS             complement(10494..10910)
                     /locus_tag="AC16O9_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR6516633.1"
                     /translation="MKGFTLTELAIVIVIAAILAALVIPQFNIRDIDAAWYYEQVRSG
                     VRYAQRTAVAQRRPVFVVVEAGPRLALCYADPCGTRVTDLATGSPVLLDAPAGVALTI
                     SVSPLSFNGLGQPSSAATINVGGQTIIVNAETGYVQ"
     gene            complement(10922..12469)
                     /locus_tag="AC16O9_08195"
     CDS             complement(10922..12469)
                     /locus_tag="AC16O9_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6516634.1"
                     /translation="MLAAAAWKAERSEWGQSWLAFAVAAYAAMALLSTFATAPAYTAA
                     GLFHPLLLVLSYVVLRGCSDQDEQAIAHMTTAFGVFIGGWGLAQVGVLGAARASAFFE
                     TPATYAAVLGLILLPLLSVVAMGRRDIAMVFTVACLAAAAFAAESRGAMLALVVGAAS
                     ALILGMRAGLLQRRGVAAVLLALAIGWAGAFAIRVTPATDAPASGIEVRAESSISRLE
                     LFALAWKARHEQPLSGSGYLTFSYVLEQNRAQVPSYGESSETAFVHNDYLQTLLELGP
                     IGLLAFLGLSVLPPVQAYRRLPELPLERRAVVVAAAAALAGMSTHALVDFPFYIPACL
                     LLYGALLGALDRRLSPNAIAIPRSWRARSWYRPARAAALTIAAVVVLRPVAAEMAAKQ
                     GLRMSLAGDAQSTAFWIGAAQRLEPRDWRYHWYAGQFWEMQAARGGNPEAARLAADAY
                     AAGLAANPLEVRNLLGVISVHRQHGPRLDAPADRAAFETWLARAEALAPLNAAVRGER
                     ALLESAR"
     gene            complement(12635..12982)
                     /locus_tag="AC16O9_08200"
     CDS             complement(12635..12982)
                     /locus_tag="AC16O9_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6516635.1"
                     /translation="MRKALQAGFTLIELVVVIVILGILAAVAVPQFVDISSSARQAVA
                     DAGCGAVQSQAVIFYASTRTAVPSASIVAGVNGASTGVTIAHTGGCNFTATASGGTEV
                     ASCSVPTPGICTP"
     gene            complement(13160..13660)
                     /locus_tag="AC16O9_08205"
     CDS             complement(13160..13660)
                     /locus_tag="AC16O9_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516636.1"
                     /translation="MIPASRQRNYLQSGLYLIIVGILATVLLERLLTYAEVAEKSAME
                     ATISRLQSALYARVAYLSLRGEQEAIEALPKRSPFVSAAMRSTNYLGEFVGLPSEAQG
                     GSWLFDRLRNELVYLPRLKRYLVGLEEQPASHLRFRLQVFRAPDTGFTVVSLKPVGSV
                     RWEPAP"
     gene            complement(13657..14841)
                     /locus_tag="AC16O9_08210"
     CDS             complement(13657..14841)
                     /locus_tag="AC16O9_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6516637.1"
                     /translation="MTGRLEGPDSGAVADQLLAIGVTPVDIQPVAEPTAIEAPEWWRR
                     FSAPPITDVDVMLFSRQMYALLKAGVPIMRALTGLQQSTRNSSLAEVIGDLRTSLDAG
                     RELSAAMRGHPKVFSPFYVSVIRIGEATGELEQSFNRMFGYMEFDKEIRDRIKSAVRY
                     PIIVLVVIAVAVAVVNFFVIPAFAKIFAAQKVPLPLLTQLLIGTSNFFLAYWPAMLGA
                     LVAAIFGAQIYVGTEQGRHQWDRWKLRLPLAGTIILRATLARFARGMSLAVRAGVPIV
                     QAMSVVGEVVNNAHMALRIGQMRDGVERGESVLRTATASGIFTPVVLEMISVGEETGE
                     LDDLMDEVADMYEREVDYEIKNLAANIEPILTIALGVMVLILALGVFLPIWGLGNVLL
                     QK"
     gene            complement(14888..16585)
                     /locus_tag="AC16O9_08215"
     CDS             complement(14888..16585)
                     /locus_tag="AC16O9_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6516638.1"
                     /translation="MGRPEKIRLGDVLVAQKAISQEQLRVALEQQKKSGRRLGRVLVE
                     KGFVNDEQICEAISRQLNIQYVNLKFYNLNQDVVRRLPEAQARRFRAIVLEDRRASVL
                     VGMADPTDLFAYDELGRILQKDLEVAVVGEAALLQTVDRVYRRTEEISGLAKELERDV
                     GDSYVDFGALGAGAEDAPVVKLLQSVFEDAIQVNASDVHIEPQETALQIRFRIDGALN
                     PQTQADPKIAPALALRLKLMAGLDISEKRLPQDGRLNVIARDQKVDVRMSTMPTQYGE
                     SVVLRLLNRRATRQSLDSLGMPPQMLQRFREIIRKTAGMVLVTGPTGSGKTTTLYSAV
                     SEVNTPERKIFTVEDPVEYRLAGINQVQVNEKIDLTFARVLRSALRQDPDIILVGEIR
                     DEDTAQIALRAALTGHLVFSTLHTKDSVSTPIRLIDMGAPAYMVATSVHAVLAQRLVR
                     LNCETCNTEYQPDANESRWLAAEVGADYASHKYFHGRGCSHCNGTGFAGRIGVYEMLE
                     MTPELARAVVARDPNMFGEVARPQMQGARLTDRALELVLKGRTTVAEVLKVATQVEE"
     gene            16696..17640
                     /locus_tag="AC16O9_08220"
     CDS             16696..17640
                     /locus_tag="AC16O9_08220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6516639.1"
                     /translation="MKTFASQADLKPKKISFRKLSRNAYAFTAEGDPNTGVIVGDDGV
                     MVIDTQATPLMAREVIRRVRRVTPKPIKYVVLTHYHAVRVLGASAYRAQQIIASRRTL
                     GLIEERGQQDMASEIGRFPRLFRGAKSIPGLTWPTLVFDSALTVMMGRLEVRIMHVGP
                     GHTGGDAIVWLPSQKVLFSGDLVEYNAGIYTGDAHLEAWPATLDKLRALKPRALVPGR
                     GPALTTPAQCQKAIGYTQDFVRGLYAAAKRGARARQSLRQVYLATKKKMDPVYGSFPI
                     YEHCMPFDVSRAYDEARGIKHPRIWTARRDREMWKSIA"
     gene            complement(17721..17957)
                     /locus_tag="AC16O9_08225"
     CDS             complement(17721..17957)
                     /locus_tag="AC16O9_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516640.1"
                     /translation="MNAYEIEQIEKDVRANFRLPLAFSEWGDIERRAREERARVLGGG
                     IARFFAAAWAKVTFLGRGMRATAADCTSARLHHS"
     gene            18069..19043
                     /locus_tag="AC16O9_08230"
     CDS             18069..19043
                     /locus_tag="AC16O9_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6516641.1"
                     /translation="MHNVVHGMLVFSKVVAAGSLSAAARELGVSPAVVSRTLAALETR
                     LGVRLVNRTTRSLHLTDEGASYYETSQRILAEIDEADAAVTSHRVEPQGVLRVALPAS
                     FGHQHIAPLVPRFAERYPKVELALSLSDRSINLIEEGFDLAVRIAELKDSSLAARKLA
                     PNRRVVCASPAYLRAHGEPKTPQDLAKHNCLVASWEHGFAMTWEYKSPTGKRGTVRVT
                     GRYACDNWEVLRDWAVAGLGVALKSTWDVRRQLEEGSLVPLLSGYDFGTDVGIYAMYP
                     HRRHLPAKTRAFIDFLAESFGPEPYWDRPAGAAPATPPAPRIKSRAAV"
     gene            19082..22621
                     /locus_tag="AC16O9_08235"
     CDS             19082..22621
                     /locus_tag="AC16O9_08235"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR6516642.1"
                     /translation="MNAPVDARKLAKVSLDDKYTLGNGRVFLTGTQALIRLLMLQRER
                     DQRAGLNTAGFVSGYRGSPLGGLDQSLWRAQKHLEDHHIRFQPGVNEDLAATALWGTQ
                     QVGMFPGAKYDGVFGMWYGKGPGVDRCGDVFKHANSAGTSKHGGVLVLAGDDHAAKSS
                     TLAHQSEHILKACCIPVLNPSHVQDYLDFGLHGYAMSRYSGCWIGFKCVTDVVESGAS
                     VIVDPKRVQVKLPTDFAMPQGGLNIRWPDGFLEQETRLLDWKVYAALAYVRANGLDRI
                     VWDAPEARLGIITTGKSFGDTMQALADLGIDERVARDIGLRVYKVAMSWPLEPQGARR
                     FARGLEEILVVEEKRQLIEYQIKEELYTWEAGKRAPRVVGKFDDNGEWSRAEGQPAGT
                     WLLPAHYEHTPAIVARAIAKRLEKLGLAERLGAQFKERLAFLDFKEKALAKPRVTAVR
                     QPYFCSGCPHNTSTRVPEGSRATAGIGCHFMAVWMNRNTSTFTHMGAEGAPWIGQAPF
                     TAEKHIFANLGDGTYFHSGLLAIRAAVAAKVNMTYKILYNDAVAMTGGQHHDGPLDPA
                     MISRQIAAEGVKPIVLVTDEPEKYPAGTNWAPGVQIRHRDELDAVQRMLREQPGVSAL
                     IYDQTCASEKRRRRKRGAYPDPAKRAVINEMVCEGCGDCSAKSNCLSVEPLETEFGRK
                     RTINQSTCNKDFSCVKGFCPSFVTVEGGQLRKGKKGSRAAGEDFPALPEAAPAPLDEP
                     CGVLVTGIGGTGVITIGQILGMAAHLEGKGVAVLDMSGLAQKYGAVMSHVRIASSADQ
                     VHVARLGTGDARLVIGCDLVVSASADALDRMSAGRTRAVVNGTTSPTAAFVSNPDWQL
                     PGSNLQHDIAEACGGQGVEFVPAAEVATALMGDSIATNMFMLGYAWQKGWVPLGRDAV
                     ERAIELNGVAVEFNKQSFLWGRRAAVDLERVRRIAMPADVIPIDQHVSRNLGELVERR
                     VQLLTAYQNAAYAARYRALVERVRAAEQDAVGGTKLAEAVARYYAKLLAYKDEYEVAR
                     LYATGEFMKKIDGMFEGGYKVNFHLAPPLLARPDPLTGEPRKMQFGPWMLRAFGLLAR
                     LKGLRGTPLDVFGRTAERRMERALIGEYENTVQTLLGGLSRDHHALAVEIASLPESIR
                     GFGHIKAKSVEAARRKQAELMARYRTAPVKAAA"
     gene            complement(22682..24169)
                     /gene="ubiD"
                     /locus_tag="AC16O9_08240"
     CDS             complement(22682..24169)
                     /gene="ubiD"
                     /locus_tag="AC16O9_08240"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HIR6516643.1"
                     /translation="MHYADLREFLGGLEALGGLKRIAAPVSPRLEMTEICDRVLHAGG
                     PALLFEQPAGFALPVLGNLFGTVERIALAMGVDAGTDPRPALRDIGRLLAALREPDPP
                     KGLRDAVTKLPQLARLAGKVFAMAPKTVSSAPCQETVWEGKDVDLARLPVQTCWPGDA
                     GPLITWGLTVTRGPLKPRQNLGIYRQQVIGANRVIMRWLAHRGGALDFRDHQLAQPGA
                     PFPVAVALGADPATTLAAVTPVPDALGEYQFAGLLRGARTELAKCLGSDLQVPARAEI
                     VLEGAIHPGDTALEGPHGDHTGYYNEAERFPVLTVERITLRRDPIYHSTYTGKPPDEP
                     AMLGVALNEIFVPLLAKQFPEIVDFYLPPEGCSYRLAVVSLKKQYPGHAKRVMFGIWS
                     VLRQFMYTKFIVVVDDDIDVRDWKEVVWALSTRVDPARDTLIAENTPIDYLDFASPAA
                     GLGSKMGIDATGKWPAETSRTWGRPIAMDAEVKQKVDAMWQALGL"
     gene            complement(24173..24700)
                     /locus_tag="AC16O9_08245"
     CDS             complement(24173..24700)
                     /locus_tag="AC16O9_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516644.1"
                     /translation="MALETPIANYEFSTGPLRGTQLSLYGTRLLHHGVGQMDSMALGA
                     IAAVRVGYERDVRRIAGGVALALLALVLFAVAGPLSGAAAEAAAEVSGNHSVAQLLRG
                     TLLVLGAIASLLPAVALGCLLGGGALAAFGWIGTTTLVLSLPAADRAYAVRGQNRMLV
                     DFAELLAERVAQSGR"
     gene            complement(24710..25582)
                     /gene="ubiA"
                     /locus_tag="AC16O9_08250"
     CDS             complement(24710..25582)
                     /gene="ubiA"
                     /locus_tag="AC16O9_08250"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR6516645.1"
                     /translation="MSWTAIEQRLDAYERLLRLDKPIGTLLLLWPTLWALWLAARGLP
                     PVELIAIFCVGTLLMRSAGCAVNDFFDRGFDRHVERTRVRPLAAGEIRPAEALVLAAL
                     LAAAAFGLVLMLNRLALQLSFVALGIAITYPLTKRFFALPQLYLGVAFGFGIPMAYAA
                     AQSRLPAECWVLLAANVCYAFAYDTEYAMVDRDDDAKIGVRTSALTLGRYDVAGVMAA
                     YAGMLALLGAVGVMARLGWPYYAGLALAAALMAYHYRLIRDRSRTGCFKAFRHNNWVG
                     AAVFAGIAAHYGLR"
     gene            complement(25595..27601)
                     /gene="recG"
                     /locus_tag="AC16O9_08255"
     CDS             complement(25595..27601)
                     /gene="recG"
                     /locus_tag="AC16O9_08255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6516646.1"
                     /translation="MTRALPPATASKLAKLGIRSRHDLLFHLPLRYEDETRLTPLERA
                     APGAPLLVQAPVRRAEIAFRPKRQLVVHADGLVLRFLNFYGSQVKQFERAAREGLLVR
                     AYGEVRPGWFGAEIVHPRYRIVAPDAPLADTLTPVYPTTAGLPQATLRKLVLEALAEE
                     PIEEILPASLRERLGLAPLAASVRLLHGPAPGADTASAWQRVKFDELLAQQLQLRLAR
                     RKRRERSAPALPASGPLLKRFLGRLPFRLTRAQARVMAELLRDLGQAHPMQRLLQGDV
                     GSGKTIVAAIGCLAAVDAGGQAAVMAPTEILAEQHYRKFSTWLGPLGVSIAWLHGGLP
                     AAERRRARKSIASGAASIAIGTQALFQEGVEFSRLALAIVDEQHRFGVKQRLQLRRKS
                     EQAAAHQLMMSATPIPRTLAMSYYADLDVSVIDELPPGRKPVTTRLVAAARRAEVLER
                     IRAACADGEQAYWVCPVIEESKDGDLQAALEAYEELRQELPRLRIGLVHGRMAPAEKA
                     AAMQAFAEGATQLLVCTTVIEVGVDVPNASLMVIEHAERFGLAQLHQLRGRVGRGARA
                     SHCVLLFQEPLSDGARARLKVIYENADGFAIAERDLVLRGPGELLGVAQSGAPLLRFA
                     DLVADEPVLEAARAAADELLAADEGLARRHIDRWLGLRADFGKV"
     gene            complement(27598..27975)
                     /locus_tag="AC16O9_08260"
     CDS             complement(27598..27975)
                     /locus_tag="AC16O9_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR6516647.1"
                     /translation="MKTAIQTAAAPAAIGAYSQAVRAGSTVYLSGQIGFDPATMQLAE
                     GIDAQIHRVFANLAAVAQAAGLGLGHSVRMTVYLTDLAHFAKVNEIMAQYVPQPYPAR
                     AAVGVASLPRGALVEVDAILHAG"
     gene            28018..28347
                     /locus_tag="AC16O9_08265"
     CDS             28018..28347
                     /locus_tag="AC16O9_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516648.1"
                     /translation="MTTPLIASLEKLLGTPRDGALLRYSLGSECLKAGSLERGIEHLR
                     EAVRRDPQYSAAWKVLGRALAAADRVQEAREAYARGIEAARAKGDRQAEKEMAVFARR
                     LERRSGA"
     gene            complement(28344..34607)
                     /locus_tag="AC16O9_08270"
     CDS             complement(28344..34607)
                     /locus_tag="AC16O9_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR6516649.1"
                     /translation="MTQVSALKAEDLRPAALRLLMVEDDANDAELLQSHLASARLGGA
                     EILHARSLAEGLALLQSQDVRITLLDLDLPDSQGLQTLERVRAVARGPVIVVSGNDHP
                     SLVDEALKRRAYDVIPKDELDAATLRRVLRLAIRHPQAGREGASEGRYRALLESSSEA
                     LVLLDAKGRIEYASAAMRRVLGFNTAEILGRAGLSFVVPDDRPAVQQAFGALSAQPGS
                     FVTLRVRFLHKNGGQRVLESSLANRLGDADVAAIVCSYRDVTQEEEHRERFAATFENS
                     PVGLAHVDLEGRFRLVNRRLCTMFGYTSEELIGRPVRGLSHPEDSAAADALHRQLRAG
                     VVPQFTTRKRYLRKDGATLWVQLTVSLERDAQGAPKYDIAAFEDVTDAVRAEERLRAS
                     ESRLRAIVGTEPECVKLLDAEGTVLEMNPAGLAMIEAESLAAVAGQCVYALVAPAYRQ
                     RYKDLTDRVCRGERGQLEFEIVGLKGTRRWMEMHAVPLQDAATGKTLALSITRDITAR
                     KRAELGAERVRRMYAALSNANEAIAKSEDEPSLYRRICELLVEHAGLKLATVRLVDRN
                     THWMELAAHAGGPMSYLEKVRVSIDRARPESRGPAVTAVLENRTLVHNEFLAEPTLAH
                     WHAPAREAEIAATMCVPLRRGGEVIGVILLYAAETGWFDPELVGLAERMAQNVSFALD
                     NLEHERQRSAAQAAVKESEQRFRSLVALSSDFYWETDAEHRTTKREGGGRASAQLTDP
                     GIGKRRWELPALAPDVAGWRAHKALLDAHQPFQGFEFCRPTPDGGSRDFSISGEPVFD
                     EQGAFRGYRGVGTDITVRKSALRTLELEHAVNRHLAEAESALAGVQAVIRAICELHQW
                     HHGRFWQVDDEAGLMRFAAHWSREGGGADAFTSALRDVVFQPGQGIAGRVWQSGEPIW
                     VADASKDARAAKHTLWRTPLRGTFMFPVKGAGKVLGVITIASEEVRKPDERLLAAAGV
                     IGDQIGQFLLRKRNEEAMRRFRAGMDASADMVLLIDHKAMRYVDVNETACRTLGYSRE
                     EMLAMGPHDLLPAPREVLQAEYAGFVADPSTIHGMNSFYRRKDGSTIQFESTRRVLQS
                     ENGALIVAISRDISKRLEAERALRESEERFRSLTELSADWYWEQDAELRFVGTGGQDK
                     ARGGITAAQHDGLRRWELPGTEPLEGGWEAHKAQLEARQPFRDFMLKRKGNDGRTHYV
                     SISGEPILGEDGVFRGYRGIARDVTETMQATMALRRFRAALDISMDAISLIDADTLCI
                     IDINDAALRSLGYERHELLGRPVTAVFPDRSAEDMRAAYDKLLQDPHGSELLQMRHRR
                     KDGSIVPVEVTRRVLRTGEGTYIIGIARDLTERLRSEERLRASHERFEMVSRATNDVV
                     WDWNLVTNQVWWNENFQRLFGYAPQEVGAYADFWISRIHPDDAARVKSQVDTAIRGGA
                     KAWSGEYRFLCKDGGYVDVFDRGLLMQDERGQPVRMIGAMMDISERKHAEQHVRVHAE
                     RQAAIARFGQFALGRRVTEELYAEAARALRCEGVDAACVLEMFTAKGEYLVRAAQGEG
                     PHGMLGVRGPMAADSVWPEILRENTPRIAGRKYLSSRPLDRPWHAWVRTMGSAMYVPV
                     RDDASPIAMLCVYALEERAFSTEDLRFAESVGHVLSTALQRQKAEQRLAHLAQFDALT
                     GLPNRTLLQDRLAQTIVQSRRKRWHAGALFVDLDRFKLINDTLGHHQGDALIRQVGER
                     LLECVRPGDTVGRISGDEFAIVLADLARPDDAALVTQKVLDALAQPFDLGGNEAYISA
                     SIGIAAFPEDGDDAETLLKNADIAMYRAKESARNCYRFFTTEMNQRTVAKVQLNTDLR
                     RAIEREEFTLHYQPKVDLADSHLRGLEALLRWDHPQRGVVSPGEFIPALEDSGLILPV
                     GEWVINKACAQLRRWRDEGRSVVPVAVNLSPKQFRRSDLDRIIRDLLARHDVEAGLLE
                     LEITESCLMEDPEQAVRVMHNLRAAGLCISIDDFGTGYSSLSYLTRLPLSALKIDRSF
                     VRDAGASAEAASIVRAVIDMAQNLRFTVIAEGVETEQQVAFLRRHGCNQAQGYYFGRP
                     DCAEVAGAHLPRA"
     gene            complement(34604..37042)
                     /locus_tag="AC16O9_08275"
     CDS             complement(34604..37042)
                     /locus_tag="AC16O9_08275"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF/EAL domain-containing response regulator"
                     /protein_id="HIR6516650.1"
                     /translation="MAPSAPPARIIVLDDNPGFAGAARRFVERLPGYAHATGDVVLVD
                     LGIAGLDLARRLKRSNPALGVIALALFPTAELLAEAERAGVDAVVSKEAFAEELPVAL
                     ARAGVVLGAALADGPAQESLRESEERFRRLTQLSSDWYWEQDAQLRFVHTGGDAAERG
                     GITAEQHVGLQRWELPGTTPLEGDWTQHRAVLAARGSFHDFMIKRSVAGQRVAYVSVS
                     GEPVYDANGVFRGYRGIARDVTESHEAMLALRESERRLSTLIASLPGAAYRCRNDEAW
                     TMEFVSEGTRLLTGYTPEDFTSGRVAYGSLVHEEDRERCWHSTQEALRQRAPYTMEYR
                     IRTADGAEKWIWERGVGVQDAKGEVVALEGFVSDITERKLTEQRLAQLAQFDTLTGLP
                     NRLLLHDRLAQSLTQARRHDRKVGVLFVDLDRFKLVNDSLGHYAGDLLIAEAARRLAG
                     CVRPGDTVGRISGDEFAVVLADLAHADDAGLVAQKALEALGEPYQLAGSEAFATASIG
                     IAVYPGDGYDAEDLLRNADMAMYRVKESTRNAYCFFTAEMNQRSAQKMQLNNDLRHAI
                     ERGEFVLHYQPKVALDGGALTGFEALLRWDHPTRGLVPPGQFVPALEESGLIVPVGEW
                     VLREACAQIARWQQAGFAPVPVAVNLSAKQFRRRDLDEVVSGVLAASGVTPALLELEI
                     TESCLMEEPDQAVRLLYRLRDAGVRISVDDFGTGYSSLSYLTRLPITALKIDRSFVRD
                     AVTNAEAASIVRAVIDMAHNLRFTVIAEGVETPQQVAFLRKHECDQAQGYHFACPALA
                     EQNSRWLESARFAA"
     gene            complement(37143..37793)
                     /locus_tag="AC16O9_08280"
     CDS             complement(37143..37793)
                     /locus_tag="AC16O9_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516651.1"
                     /translation="MAALRVLLVDDHSLVRAGMRSLLRDIADVEVVAEASDGAEALAA
                     AERERPDVVLMDIAMKGMNGLEAAARLRERQPVAKVIILSMHTSEEYVLLALRAGAAA
                     YLIKDSATSELELALKSVMRGETYLSPAISRQVVDGYVQRVGVGAGPDPLTPRQREVL
                     KRIAEGCSTKEIAFELNLSVKTVETHRAQIMERLSIRDVAGLVRYAMRAGIVPPET"
     gene            complement(37796..39253)
                     /locus_tag="AC16O9_08285"
     CDS             complement(37796..39253)
                     /locus_tag="AC16O9_08285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6516652.1"
                     /translation="MARLRVLLLEDSPADAELALAALAAAGLDCAADIAQAHADFEQL
                     YAADRYDVVLADYSLPDYTGLDALARVRAIDGLQPFILVSGALGEERAVEALRAGATD
                     FVLKGNLNRLGPAVARALQENRQRREHLATQRALELSERRYRAIVEEQSELIIRMLPD
                     LTVTFVNGAYCRYFGRTQAQVLGKSLLEHMRPEERGNAQRFLAAIGPSTPGYERDALV
                     RDAGGAERWMHWATRGLFDADGALREIQSVGRDVTELKRAMDALGAARERLQWLSRRL
                     LEVQEAERRHLARELHDDIGQGLTALKLNLEALARGRDGAALATRIAEALETARHTIE
                     RVRQLSVNLRPLQLDDLGLAAALRSHLDRQARLGGLAPHFEIQEVPLKLSPDVETACF
                     RVAQEAINNIVRHARAANVWLRLFLAGERLALSVRDDGAGFDVTWAQRRAAAGESLGV
                     VSMEERVALAGGTLQIHSAPGQGTVIVASFPVGAP"
     gene            complement(39258..39461)
                     /locus_tag="AC16O9_08290"
     CDS             complement(39258..39461)
                     /locus_tag="AC16O9_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516653.1"
                     /translation="MAFRLRPRSIHGELALLVGVPVLPFVAMTAYLLYQQAQRDLEEA
                     RSVVRELGPLWMRVNEPPPRACG"
     gene            complement(39653..39728)
                     /locus_tag="AC16O9_08295"
     tRNA            complement(39653..39728)
                     /locus_tag="AC16O9_08295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39693..39695),aa:Ala,seq:cgc)
     gene            39903..40583
                     /locus_tag="AC16O9_08300"
     CDS             39903..40583
                     /locus_tag="AC16O9_08300"
                     /inference="COORDINATES: protein motif:HMM:NF018928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HIR6516654.1"
                     /translation="MRLQRAGEAPAFTRALNAIPISYTEFAPAARDYPLVFTSADGGK
                     TYAPVAVLGMRQGENLFSAQDAWAQDVYIPAYVRRYPFCMARVRVQDVERQDRLICVE
                     KACLEDAGGEALFDDKGEPLSRWRDLQRLLVEYETDLERGRELCTILADYALLQPFNM
                     QAKLDQGAQMQLTGMHRVDETKIEHLNPSQFKNLARKGILGRIYAHLLSLENFARLLR
                     RRAARASG"
     gene            complement(40588..40986)
                     /locus_tag="AC16O9_08305"
     CDS             complement(40588..40986)
                     /locus_tag="AC16O9_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516655.1"
                     /translation="MSGGHDSEGEVLAQWLKEAGRRAQSPARSEAKANGDDTYNSPAS
                     LHRYVEELAGRKLQTHAEVLEFLKEVAGSQPQGHREHERRRMVREVALIVALALSYLH
                     FYYWEVQLEIAALNSVRVVLPAIAVRTKSI"
     gene            complement(41056..41835)
                     /locus_tag="AC16O9_08310"
     CDS             complement(41056..41835)
                     /locus_tag="AC16O9_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516656.1"
                     /translation="MAQKARTYRLTAAGRAAWESQDMAVPEDYRRILWLMDFHGQDGV
                     VGELLRRYPRNVLNEWLSEMKDLGLIESSLEGEDDASAFSAGESDLTLGLDQARMRRD
                     GDSASAALARTGAYISADRLSRRPAARKAPADTVVLIVEDDPDQLALADLRVSMAGYK
                     VRVAKSVNEFLHSMLDEGAPDLLLLDVILPDGNGFDLLTKMRRHAVLGSLPIVMLTAE
                     NDAADIGKGLLLGADGYITKPYTKNIVADVIRRVMKQDGGV"
     gene            41948..42685
                     /gene="trhA"
                     /locus_tag="AC16O9_08315"
     CDS             41948..42685
                     /gene="trhA"
                     /locus_tag="AC16O9_08315"
                     /inference="COORDINATES: protein motif:HMM:NF011669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR6516657.1"
                     /translation="MLAVAGMSDPVSSMTHLAGALACATRAGALLRSGRGSGARLLSL
                     GLFAFCCVLLLALSGAYHLLAPDTAAKALLKRLDHAAIFMLIAASFTPVHAILFRGAW
                     RWGMLAGIWSAAGAGLIVKTAYFDVMPEWVGLSMYLGLGWLGVISAVALARRYGLRFI
                     QPVLWGALAYTAGALAEFHRWPLLVPGFVGPHEVLHLAVLAGIACHWVFIARIAASGK
                     SGPGWIRTNDQGIIRESRPVLDSKPQH"
     gene            complement(42720..43055)
                     /locus_tag="AC16O9_08320"
     CDS             complement(42720..43055)
                     /locus_tag="AC16O9_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516658.1"
                     /translation="MHPITVRTLQFDVPSAADFDPLYLAGSPALSYNHTAFGLYVAHL
                     EPFAVKSLRRVLDRVRDDALREAWARSGRTRRQRSAWRFLVRLGSIPWHTLGTRAGKK
                     RQQRAALGS"
     gene            43209..43643
                     /locus_tag="AC16O9_08325"
     CDS             43209..43643
                     /locus_tag="AC16O9_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516659.1"
                     /translation="MRTLWLVCALAIALVPGCAINREGASVSPDTDLSQIRKYHVVRF
                     APDARGIEAVIAGALRKRGLEASSGGEDAAPGDVQALVTYQDRWRWDITMYMLELKIY
                     IREPQTGRLLAMGSSHHTSLSRRTPEEMASEVLSNIFKEARK"
     gene            43640..44269
                     /locus_tag="AC16O9_08330"
     CDS             43640..44269
                     /locus_tag="AC16O9_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516660.1"
                     /translation="MTAPGEWTGRQWARVAMSLAFLLLAAGCAPGMQNVRVELPTYQA
                     PRAARAAEGRPTVRVEAVKDSRSDSVGSHIGERTTIGNISMGSIELEPPPTDVIAALL
                     RAEFAGMSHGLVKADERFTVAAQLRKFQVLTPATALYWDLNGAIELDLSVRSREGRTH
                     EARYAATCTDRTYLFPSEDLIRKVVAACIGRVGESLRGDAALAKFMGGP"
     gene            complement(44782..44855)
                     /locus_tag="AC16O9_08335"
     tRNA            complement(44782..44855)
                     /locus_tag="AC16O9_08335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44819..44821),aa:Ile,seq:cat)
     gene            complement(44937..47186)
                     /gene="rpoD"
                     /locus_tag="AC16O9_08340"
     CDS             complement(44937..47186)
                     /gene="rpoD"
                     /locus_tag="AC16O9_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR6516661.1"
                     /translation="MVKAKRKAKSKQPRKAGKKAKAIKVLKKKARPTSKARAHTKKPL
                     KGKPARLAPKKVHKKVHKAAPSRAPAAAKIDDKAKKPTIKSAKAALEQLEAKLAEGLK
                     SKAARGRPGRKSKKEEWLAAMAVLPKEDAETRRTRLKTLIKLGKDRGFLTYAEINDHL
                     PDDLVDAEQIEAIISTFGDMGIQVYDQAPDQEQLLITAESAQVAPTDEDVEEQAEAAL
                     STVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEEGLRHMVQAISACPTTVR
                     EILGLAEKVEKEEMRIDEVVDGLIDPNAKDDFDPKKAEAASEEEEEEQIEEEESESEE
                     DEGAAQTASLLALRNEALEKFVRIRRLFERMQRVLENKGSKDKDYLRVRDQISAELMT
                     IRFTARTVERLCDSVRSLVDDIRSHERQIHDISVNKALMPRQHFIKVFVGTEGSKRWL
                     SGEVNANHPWSEQLARVEPAILELLGKIQGVQQKIGISIRDLKEINKQMSTGEAKARR
                     AKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYAT
                     WWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGQEPDPATLAQKMEMP
                     EEKIRKILKISKEPISMETPIGDDDDSHLGDFIEDQSTLAPNDAAMYASLREVTKEVL
                     DTLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSER
                     LKSFLDQEG"
     gene            complement(47256..49010)
                     /gene="dnaG"
                     /locus_tag="AC16O9_08345"
     CDS             complement(47256..49010)
                     /gene="dnaG"
                     /locus_tag="AC16O9_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6516662.1"
                     /translation="MIPDSFKQDLLNRVDIVDVVGRYVQLKKAGANLSGLCPFHNEKT
                     PSFTVSPAKQFYHCFGCQKHGNAIGFLMDYGGMGYVDAVKELASMVGMQVPEWQPRTP
                     EEAKRKERETDLAALMQKAMEFYRAELKRSPRAIDYLKRRGLSGETAARFGLGYAPDD
                     WQGLKRAFPDYENAALVECGLVIEGEGKRYDRFRDRVMFPIFNRRDAVVGFGGRVLGE
                     GEPKYLNSPETPLFQKGQEIYGLGHAREAIRETKRALVVEGYMDVIGLAQHGVGYAVA
                     TLGTATSPVHVTRLLRELRESGSELVFCFDGDSAGRKAAWRALEVSLPLATDTTAIRF
                     LFLPEGEDPDSFVRAHGQQAFERLMSRAQTLSGFLLGELRAQSDLAAPEGRARFQVTA
                     KPLLQRLAAPALRQQLIRAVAELAQVAPEYAENFFAPEGEARPRRPSVARAQAPILKS
                     EEQHLLRCVVTHPALAEELDEELLDPDLPESAALRSLAADLAGAEGASGAVLVERYQG
                     TQFEKAVFQAQQSAIEQGVTPESAAQEFRQLQIALRIRRKHLEIEALKGQVEANPALN
                     AELHLRVKELHQLKAQRS"
     gene            complement(49077..49523)
                     /locus_tag="AC16O9_08350"
     CDS             complement(49077..49523)
                     /locus_tag="AC16O9_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6516663.1"
                     /translation="MTLKERIQEDMKAAMRAKDAARLSAIRLLLAALKQKEVDERVAL
                     TDADVLGIIEKMLKQRRESITQYEKAARQDLAEQEKFEIGVLGAYLPQQMGEAEIAQA
                     VAAAASEAGAAGLKDMGKVMALLKPRLAGRADMGKVSALVKTKLGG"
     gene            complement(49595..49807)
                     /gene="rpsU"
                     /locus_tag="AC16O9_08355"
     CDS             complement(49595..49807)
                     /gene="rpsU"
                     /locus_tag="AC16O9_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR6516664.1"
                     /translation="MPTVRVKENEPFEVAMRRFKRTVEKTGLLTELRAREFYEKPTSE
                     RKRKLAAAVKRHHKRLRSQMLPAKLY"
     gene            49856..>50743
                     /gene="tsaD"
                     /locus_tag="AC16O9_08360"
     CDS             49856..>50743
                     /gene="tsaD"
                     /locus_tag="AC16O9_08360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6516665.1"
                     /translation="MLILGIETSCDETGVALYDAAAGRLLAHVVHSQVQMHEAYGGVV
                     PELASRDHVRRLVPLARDVLARSGRAVAQLGAVAYTQGPGLAGALLAGAAFAHALAAS
                     LGVPAIGIHHLEGHLLSPLLSARRTAFPFVALLVSGGHTQLMRVAAVGRYTLLGETQD
                     DAAGEAFDKTATLLGLGYPGGPALARLAERGHAGRYRLPRPMIASGDLEFSFSGLKTA
                     VATLVKGGGPDAQFHADVARAFEDAVVEVLVAKCALAMERTGLTQLVVAGGVGANRRL
                     RAALDARGKSQGFDVVYPEP"
BASE COUNT         8133 a        17491 c        16804 g         8315 t
ORIGIN      
        1 ccaccgtact tcttggccaa aatgtgcgtc atcgccgccg tcagcgtcgt cttgccgtga
       61 tccacgtgcc caatcgtccc cacgttcacg tgaggcttcg tacgctcgaa tttgcctttt
      121 gccatgacct tctttcgtgt ccgttcagtc tctacgcgct gccgttgtcc atggtgccca
      181 tgatgcggat tgaacgcatg acctctccct taccaaggga gtgctctacc actgagctac
      241 atgggccgtt atgctctgct ggagcgggtg aagggaatcg aaccctcgtc ataagcttgg
      301 aaggcttctg ctctgccatt gagctacacc cgcctcgttc acctcgaccc acgccatgtg
      361 ccgcctcacc gccgggaccg cccggatgct ggtggagggg gttggattcg aaccaacgta
      421 ggcgcaaggc caacagattt acagtctgcc ccctttagcc gctcgggcac ccctccggcg
      481 gaacccgaaa ttatgtgaga aatcctttgc ttagtcaagg aatcgcgggc gaaactgcgc
      541 tggcgcagtt gcgagggccg cggcgttgcg ctacggcttc agctcgcgag cgccatcaag
      601 gacttaggcg gttctcaggt gccgtatcgg acggcgcccg cgagggcgcg caaggccgcc
      661 agtgcgctgt agcaggcgag ccaagcggtc ttcgggttgt cgggcgaggg cacgttctcc
      721 agcaccaccg tgaaccgccc gctcttcccg gcgacccgga tcgtgtgcgt gttgtgcgtc
      781 agcgtgggat cggcgacgac ctcgagctcg gtgcggtcga aaccgatgcc ggcaagcgag
      841 agcaccgccg caatgtttac gttctgcgga aagtgcggca cgccagcgcg cgccgcgccc
      901 ttgaacagcg tgcgcggctg cgttacgcga tcgagatcga tgccgagcgc ctccacgaac
      961 ggaatccctt gccacgccct gggcggcttg gccacccgga tgctgacttc gtccactccg
     1021 gcaacgcacg ctgctttcag cgcgtcgagt ccgccgatgc cgcccgaggg cacgtagagt
     1081 tggccaccgc ctcgacgggc cgcatcctcc gccgcgtcgc gcaaccggtc atccgccagc
     1141 gcgcccgccg agagcaccac cacgccgacc cccgcctcga ggaacggcac cagattctcg
     1201 cgcacggcgt cgtgggacgc cgcctcgacg acgacttgag gttgcaatgc gagcagcgct
     1261 ttcccgccga cgacgtacgg cagggcgtac tccctcgcca gcgcgacgcc gcgcgaggcg
     1321 gaggtgcgac cgccaatgcc gacgacctgc atgccgggca ggtcgccgca gcgcaagtgc
     1381 tggagaacga ggcgggcgat ggtgccggcg cccagcaagg cgattcgcat ggccgcgcga
     1441 ttctgccaca gtgcttgggg ttaccgggta ctggcagtat tattccgccc gaagggagcg
     1501 cgcgatgcgg atatctattc tcgaggatga tccagatcag ttggcgttgc tcaagcgctg
     1561 gctggccgat gacggtcacg acgtgcatgg ctatctgagt ggccgcgatg ccatgaagca
     1621 tgccggtcgg gaaagcttcg atttgttcgt catcgactgg caggtacccg atgtatcggg
     1681 cgtcgacgtc ttgatgtggc tgcgcacgaa cgtgtcgaag caggttccgg tgctgttcgt
     1741 gaccgtacgc gatgccgagc aggatatcgt gttcgcgctc gagaacgggg ccgacgatta
     1801 catgatcaag ccggtgcgcc gccaggagac gattgcgcgc gtgcacgcgc tgctgcgccg
     1861 cgcgtacccg cgggtggagg agaagctcct gtccttcccg ccgttcgaga tcgatacagc
     1921 aaagagcgag gtgcgccgca acggggaacg catcgagctc acgccgaagg agtacgagct
     1981 gaccgtggtc ctgttccgca atctggggcg actgctgtcg cgcggccacc tgcaggaggc
     2041 ggtgtggggc cgtagcggcg acctcatcac gcgcacggta gatacgcacg tgagtcaggt
     2101 gcgcaagaag ctcgacttgc gggccgagac cggattccgg gtggtgccga tctacaacta
     2161 cggctatcgc ctcgagcagg tcggcgaagg caattccagc gtcgcttgag ctacttcagc
     2221 tgtctagcgc gctgatccgc gtacgcgcgt acgtcgcccg tcagggttcc cgaggccacg
     2281 ccacggcggt aggcctccgc cgcctcgggc aaatgcccct gtgcctccag cgagatcgcc
     2341 aagccgaccc acgcggcgcc actctgcggg gcggaggcca aggcaagtcg gtaggcatcg
     2401 gccgccgcac cgtgccgccg cagctgctgc agcactgccc cgtgcagcgc gtgaaagtcg
     2461 gggttgccac ggccggccac cgccgcctgt tccaagaccg acagcgccgc cgaaaagtcg
     2521 ccgcgatcga catggatgcg cgccagggca acggcgaacg gcgcataacc tgaattgaac
     2581 gccagccctt cctgcaggtg gcgccgagcg tcatcgatgc ggccttgctc gatatggagc
     2641 gcgaccagcg tctggcgtgc cgcctgatgc gcagcatccg catccagggc cgcgagcaag
     2701 gcgtcctcag cgtcggcgac ccgtgcgcgc tccagcatct gcgccgcacg gcgaaaatga
     2761 ttctctgcgc ggtcggccgg cgaacgatcc cgttcgcgct tttcgatgtg cgccggcgct
     2821 acgcgcgcgg gctcggcacg cggcttcggt gcggctgcaa cgcgcggcgt ctgcttttcc
     2881 gaaggagtcg gggccgctgg cctctgcggg cgcggcgcca gcggcgtatc gatggtgagc
     2941 gcaaggcgca gcatttcgat aggcgcggca agcttttccg gtgccggctt ggctgcaact
     3001 gcctcgagcg gaaccggctg cgcgtcggcg gcaggagctg ccgcgagcac catcacggcc
     3061 gcggtgtctt gcggagccgg tgccgaaact gtgcgctggc gactgacgtc ctcggcgcgc
     3121 gcggcaagct cggtcgcgat ggggcgcggc tgcagctgat acaccaccca caatgtccac
     3181 gcggccgcca ccagcatgag tcccgccacg atgcgccaga accactcgcc ggaaccgtcc
     3241 gcgccacgca ccgcgcgcac ctcggggtgc agagattgcc cgggcggcgc cgatcgcgcg
     3301 tgacggcgat cgaggtcctg caacattttg ttgataatgc tcatcggctg cttttagtcc
     3361 gcggcgcggc tggtgcgcag cagccaccac caggcgctga ccgggccggc cgagggagta
     3421 tcgaccgccg cggcatgcac gtgacggcgg gacgcacagc gcccgccttc gccgaacacc
     3481 agcaatagcg ccttatgggc gaggatgttg acgagcctcg gcacgccgcg gcttgcccgg
     3541 tgcagcgccc gcagcgcgcc cgcatcgaat agcggcccgc ccacgagccc ggcaatcgcc
     3601 aggcggtgtt cgatgtaggc gccaatctcg tcccggtgca gcccgcgcag cgcgtactgg
     3661 aaggtgatgc gctgccgcag ctggcggatc gactcggaag cgagcttgcg gtcaagctcg
     3721 ggctggccga acagcaccac ctgcaggagc ttccgcttct cggtttccag gttggtgagc
     3781 agccgcagcg tctccaaggt ctcgagcggc atgccctggc tctcgtccat gcacaggacc
     3841 acgcgtcgcg cgtggcgcgc gtgatcgagc agcacccgcg tgatgccgcg aagcagatgg
     3901 tgctgatcca cgtcgggctc caccggcacc gcgagttctt ccgcgatcgc cagcaccagc
     3961 gcacgcggct ccaggttcgg gttggggatg tacgcgctcg cccagttgct gtccagcgag
     4021 cccaggaact tccggcacag cagcgtcttg ccggtgccga cctcgcccgt gatcttgatg
     4081 aacccctcgc cattgccgag cgcgatcagc agcgtattga gcgcctcctg cgtactgcgg
     4141 catgcgtaga agaagctggt gtcgggggtg atcccaaaag gcgcttcgcg caggccgaaa
     4201 tggcggaagt agttcatcgc gagccggcct cctggggcgg acgccgcgga atgaaacgct
     4261 caaggcgctc gcgagtctcg cgcaggtcct ggccccagct ggcgtcgttc tggatgacgg
     4321 tcggcttgag caggatcacg atctcgcgct tgcgcgcctg gcgatcggaa ttacgcagaa
     4381 gcacattgga atcggcgacc cccgggatgc cagtgcggcc atcgcgaacg tccacgctca
     4441 tcaagccgcc gatggcaacg atgttgccgt cggtgacgcg gactacggtg tcggattcgt
     4501 tcaccgccga cttggcgagg ggcagcgtaa tcgaagggat ggtgcctccc aggtccacga
     4561 cgcgccggga ctcggtgacc tcgctgatcg atggatggat atgcagcgtg atgttgttga
     4621 actggtcgat ctgcggcgtg acgtcgagca tgatgccgga gaagaacggc gcgacggtca
     4681 cggtcggggt ctgctgtgtg gtcgtccccg tggtcgtggt cacggtggcg acgttggtga
     4741 cgaaaaactc gtcggtaccg accttgagca ccgctttctg attgttgagg gtggcgatgc
     4801 gcgggctgga gagcacctgc gtcgtgccct gcgagtcgag gaattggagc agcgccgcga
     4861 agttggaagt ctggaacgcc aggccgaaga ccgcgcctcc gtttcctgcc aacgtggccg
     4921 cggaggcaag cgtacctgct gcggggttga ccgtaatgcc ctggctgctc agcgtcgcgg
     4981 aactgatatt ggacccgagt gtcgtgttgt tgccgatctg accaatcgcg ccgcgcgggc
     5041 tatcgtgcgt gcggaacaat ccccaattga tgcctgactg aaagccctcg gcaagcgtga
     5101 cctcgataac cttggcctcg agcatgacct gccgctcgac caccaagcgg gtctcgcgca
     5161 gatagctttc cacggagcgc agttcggtcg gaaaggcgcg gaccagaacg acccccgatt
     5221 gaggcgttac gacgacgctg cgcccgtcgc ctgttccgac aatcgccgtc agtgcagtgc
     5281 gcaagtcccc ccagaagtcg ttgaatatgc ttgtcgtcac gcgcgtgctg tccattgcgc
     5341 gccccgagcc cgtgctcggg accggcgatg ccgtcgaggc aggcgtgggc gggccggagg
     5401 gcgccgacga gtccgcgagc gagcccgact gcacccgcag gtcgctggta ccgcggcgct
     5461 ggccgagaag atagttcacc cggtagatct tggtctgcag ccccgcggcc tgcacaaaga
     5521 tgcggttgcc ttcgatgcgg tagtcgtgcc cgtacagctc acgcagcgaa tcgagcgctt
     5581 cgcgcacggt gacgtccttc aggttgactg taatcaggcc tgtgatgccc gggttgagga
     5641 gcatcgagta acgcgttccg gaggcaatcg ccatgaacac ctgggcggcc ggcgcgttgc
     5701 tgaccgacag atcgaatctt gaatcgatcg gtcgtccttc cacgctgggc atttccatgc
     5761 gcaggggcgg cagcagcgcc tgttgcggca atcgtgtctc ctgcggtccg ggactggcga
     5821 tcgccttgtc gacctcgcca gtaatcgccg gatccacgct ggtcggtcgg ggcgggggcg
     5881 tcgcgcaggc ggccatcagc agcacaccga ggcaaaccat ggagtacttc atctggaatt
     5941 tcctttgagc aattgcgtct cggcgtcagc gcgccgctcg cgcttgacta cgccgggaat
     6001 gagatgcaac gtttccctgc ggtcggcgta ccgcagcatc acggcgcctt cggatatcgc
     6061 aactaccgtg gcgccgccaa acttctcacc caggcctacg gtgctgccgc ttatgacggc
     6121 gatgcgccgc gtcggcgaga tcaagacgga ttgaagctga ggcccgaccg gcgccgcgga
     6181 ttcgactgcg cctgaccctc ccgtccacgt gggcgggcgg gtcggatcca caagcgccgc
     6241 acgctgcgcc agcgcactag aggccatgca aatcagcgaa aggagcaaca cgcggttcat
     6301 accaccagcc acgcttgatc gaagctcagc gtgtacacgg tgagcttaag ggtcgccacc
     6361 ggatactcgg aaaccgacat ctctgcgcgg ccccaataca gctgagtcgg caaactctcc
     6421 agagccgcca ggtacgcgta caggtccagg tagcttccgg cgagcgtcag ctcgacgcca
     6481 tggcggaaca cgcgccggcc tgcctgcccc tgggttgcag ccaactccgt caccggcagc
     6541 gacttgagat cgatcaagcg cagtcgcttg tcgcgctgga gcatctcttc caggacgccc
     6601 cgcatgcgct gcggcgtggt gaagcggcgc tgctcctccg cgatctgccc ctcgagcgcc
     6661 gcgatctccg cgcgcagcac ggcgacgcgc tcgcggctgc gtgcatcggg atcgccatcg
     6721 cgggcgcgcg cgaggcgctg cacttcaccc tggatagtgc gcagctcgcg ctcgttctgc
     6781 gagatctgcg cgctcaagcg acgctgcgag tcctgcaggg gtttgatgag ggccccgttg
     6841 atgaccagca cgaggatcag ggctgccgca aggaacacaa gcacgcgctc acgcagcgtg
     6901 gcgttgtcga tgcgctcggc gtaccgcttc aacagagcgc tcactaccgc gcccccttgg
     6961 tcgccggccc gcccgcatcc accagccgta ttggggcgga aagatcgaac tcgacaaacc
     7021 gcagcggacc gcccggcgca ctgccgccac gcgagggcga ttcatcccgg gcgaccagct
     7081 tcaactcggt caccagccgt ccgcgcatca catcgtcccg attcagcgat ttcaggtact
     7141 cgggcagcag atcgggatgc aatgctctgc cgtggatgtt gaggacgttg ccgttgtcgc
     7201 cgacgctgaa tccggtcagc cataccccgc gcatggggtg ccgggcgaaa gcggcgaaga
     7261 aacgcgagaa cccgtccgta tttccgagcc ccccgccctg catgacgtcg agcacgccgc
     7321 gctgcgcccg caagctggcc tcgagtcgcg ccacttccgc ctcgagcagc ttgctgcgtc
     7381 cttgcccgct caacgcgacg aactgctcgc gctgcgccgc aagctgagcg ccgtaccccg
     7441 cggccagccg cttgaggtcc accgactgcc atagcgcata ggcgtagagg acgacaagac
     7501 cgagcacgat cagacccagg ctctgcagca tggtgcgcgc ggagaagtac ttctccttgc
     7561 gcatgaacag cgggttgaaa aggttgatct gctgactcat acgggcccta caactccttc
     7621 gactcgtgcc gcagcgcggc gccgatcaaa tggaagaaac gccactcctc cttcggctcg
     7681 agctccgcgc gagcgccgaa atcgatcgcc gcggaaaggc gcacgcgctc gacgggaacg
     7741 tcaaggttgc cagccaggta ctccagcaga cccgtttcct ccggatcggg cgcgagcatc
     7801 agcttcgcca cgggaacgta tgcgaactgg cgatcgaaat gatcgaaggt acgctgcagt
     7861 tccagcagca cgcgttgaaa cagctcctcg cgggcagccc cagacgacga tgccatttcc
     7921 tgcattccca cgtcgaggcg ccgtgccagc atcaactcgc gtcggaaatt gatcgtcagc
     7981 aatccttcgc cctggccgat gtagagcatg gcgagcccgc gatattcatc ctcgaacagc
     8041 gcggcgagat tgcgctgcga agtctcgggg atctcgatca ccgacagcgg aatgcgagcg
     8101 ctctcgaagc ggccgatgca cgcctggatc aggtcgtttc gggccgcgac cgcatacatc
     8161 gactgcgcac gcccgccggc ggcggggttg gggggcaccg caagcacgtc gaccgttgca
     8221 tcgttcacgt gatagtcgag cagatccttg atgcgccagc ggatcgcggt cttcaactca
     8281 gcctgcggca cattcggcgc ctccaccagc agcatctggt actcgccggg cacaagcaac
     8341 gtggaacact ggtatcggcc gaggccatgt tcgcgagcgg cgttctgcgc ggcatcgaat
     8401 tcgccctcga acacccgggt gccgcaaaca tccacggtcg ctgtgccgcc cagggccacg
     8461 tttccgtgca cgaaacgcaa ttccccgggt tgaaggctca cggcaagcca cccgggttcg
     8521 cgcgtggtgc gggaaaacgg ccacttcatt cggatgttgc ccccaaatcc atgatttaac
     8581 ttgaaattaa atcaataacc aattgttgtc aatgggattt ccgtaagaag cggatattgc
     8641 cattcctccg gtttttccgc tttcaccttg ccagggtcaa ctgcagttgc cgctcgacgt
     8701 agaccgcgcc ggcaccgctg gcgcaggggg tggcgttaca acccgtggaa acgatgcggt
     8761 aagtggtcac aggggtcccg ccttcgttct cggctccaaa ggacgtgcag gtaacctcgg
     8821 cgtagaagcc cgcaagattc gcggccaggg cgatggtggt cgtgcctgcc gggcacgtgg
     8881 cgttgcgcaa cacccggtat gcgccccatt cgagccccgc gcgtgccgat tggtaggccc
     8941 gggcaccctg ctcgtccatg aggcccgact caacctgcac gttcgacacc gtgagcaggt
     9001 aggcgccaat cgccgacagc gacacgatga ggaacaaggc aagaaccagg gcaaaaccac
     9061 ggctgcggcg caggaaagat ttcatggcgc gttgttcacg tggaccgcgt gatacagggc
     9121 cacggtttcc ggagttccgc cgaacagctg ccgttgcagc gtcaggcgaa gcgtaagcag
     9181 accgagttgc gccgacacgt tcggcgcgta ctcgaacacg cagcccgtca cgtgctcggc
     9241 cacgagcgcc ggggtcccgc caaacgaggt cggctgggcc gccgccagcg cgtacgcgct
     9301 atggcggtag atcctgccgg ctgcgaggtc gcactcgtaa gtcaccgggc cggaaatgac
     9361 gaagaagcgc ttgcccggcg aatcgtgcag cgcgcggacg aatgtcgtag gcgcaaatgc
     9421 aacgcggtct ctcgcgcctt cggaggtgtc gaccgcggtg atcgggacgc gattcagagt
     9481 ccccgcggtc tggtaggcat tctgatccgg aaagccgtag ttgttgagca ccagctgatc
     9541 gccggcgaca aaggcacctg gcagcttgcc gagcgtcttg aagcactgat ctccgccagg
     9601 caggtcgaaa gaaagctggt cgttgtcggg tgcggggagc gcggcatcgt cggcggggca
     9661 gttcgtgccc ccggcgggtc cgccgccgtc cgcgcggtag cggccggccg tccgtaccgc
     9721 gatgaactcc acgaacctgg aggtgcccgc gcttgcgagg cgcacgctgt tgggcaatgc
     9781 aagcttcaca tcgcgcccca tgcgctgtaa cgcgttgttc gcaatgtcgg tcagctcagc
     9841 gcgtgccgca atgtcggttc cctggcgcag gggcgtcaag aagaaactga cagcagcgac
     9901 gatgatgccg acgatgacga tcaccgccac cagctcgatc agtgtgacgc cgcgttcagg
     9961 gcgaattggg cgcatagcga aggcggtagc cctgcaagac aacttgagta ttggcgggcc
    10021 ccgtcaccgt gatggtgata cgcagcgcct cgtcggcggg aattgcggcc gggaagtcgc
    10081 cgcccgcagc gacgattctc acttgggctg aatagcctgt gagaaccgtc gcgccagtaa
    10141 tgtcctgaat gctgcccaac atgtccggcg gcccagcgta atcactgaca ttgtcgaaaa
    10201 acgtcgggcc gtaccgcgtt tccccgccct cgacaccaat gccctccggc gtcgcgcaat
    10261 ctcctacgcc tgtcgccgta aacactgcgg gatcgtccgg atcgcagaat gtgaaagggc
    10321 gcagctggat ttcctccatg agcgaactag cgatcgccac cgcctgcttg cgcaccagcg
    10381 gatcgacgct cgacagcgtg agccggttgt aaagcaccgc gagccccgcg atgccgatgc
    10441 cgagtacgac gatgaagatc accacctcga tcagcgacag gccgtgttgg cggctactga
    10501 acatagcccg tttccgcgtt cacaatgatg gtctgaccac cgacattgat cgtcgctgcc
    10561 gagctcggct ggcccaggcc gttgaacgac agcgggctga cggagatggt cagcgcgacg
    10621 cccgccggcg cgtcgagcag gacagggctg cccgtcgcca gatcggtcac ccgcgtgcca
    10681 caaggatcgg cgtaacacag ggcgagcctc gggcccgcct cgaccacgac gaacaccggc
    10741 cggcgttgcg cgacggcggt gcgttgagcg tagcgcacgc cgctccgcac ctgttcgtag
    10801 taccaggccg cgtcgatgtc gcgaatgttg aactgcggaa tgacgagggc cgcaagtatc
    10861 gccgcgatca cgatgacgat cgccaactcg gtcaacgtga atcccttcat gcgcgatagc
    10921 gtcatctggc gctttccagc agtgcgcgtt cgccacgcac ggccgcattg agcggcgcga
    10981 gcgcttcggc tctcgccagc caggtttcga acgcagcgcg atcagccggc gcatccaggc
    11041 gtgggccgtg ctggcggtgc acggagatca ccccgagaag attcctcacc tccagcggat
    11101 tggcggccag ccccgccgca taggcatcgg ccgccagacg cgcggcttcg gggttgccgc
    11161 cccgcgcggc ctgcatctcc cagaactgtc ccgcgtacca gtggtaacgc cagtcgcgcg
    11221 gctcgaggcg ctgggccgcg ccgatccaga acgcggtgct ttgtgcatct ccagccaagg
    11281 acatgcgtag gccctgcttt gcggccattt ccgcggcgac cgggcgaagc actaccaccg
    11341 cggcgatcgt caaagcggct gcgcgcgcgg ggcgatacca cgaccttgcg cgccaagaac
    11401 gtggaatggc aatcgcattc gggctcagcc gacggtcgag cgccccgagc aacgccccgt
    11461 agagaagcag gcacgctgga atatagaacg ggaagtccac cagtgcatgc gtagacatac
    11521 ccgcaagtgc cgcggccgca gccacaacga cggctcttcg ctcgagcggc agctcgggaa
    11581 gacggcgata ggcttgcacg ggcggcagga ccgacaatcc aaggaaggcg agcaagccaa
    11641 tggggcccaa ctcaagcaag gtctgcaggt aatcgttatg tacgaaagcg gtctcgctgg
    11701 attctccata gctgggcact tgcgcacgat tctgctcgag aacatagctg aacgtcaggt
    11761 aacccgatcc cgagagcggt tgctcgtgcc gggctttcca agcaagcgcg aacagctcca
    11821 ggcgcgaaat tgaggattcg gcgcgtactt cgatgccgga tgccggcgcg tccgtcgctg
    11881 gcgtgacccg aatcgcgaat gcgcccgccc agccgatagc gagcgccagc agcaccgccg
    11941 caacgccgcg acgctgcaga agcccggcac gcatacccag gatcagtgcc gacgctgcgc
    12001 cgaccaccag agccagcatt gcgccccggc tctctgcggc aaaggcagca gccgcaaggc
    12061 acgccaccgt gaacaccatc gcgatgtcgc gccgccccat tgcgacgacc gacaggagtg
    12121 ggaggaggat caatccgagg acggcagcgt aggtggccgg cgtttcaaag aacgcgctgg
    12181 cgcgtgcggc gccaagcacg ccgacctgcg cgagccccca gccaccgatg aaaacaccaa
    12241 aggcggtagt catgtgcgcg atcgcctgtt cgtcctgatc ggagcacccg cgcagtacga
    12301 cataggacaa gaccagcagc agcgggtgga acaggcccgc ggccgtataa gctggagccg
    12361 tcgcgaacgt actcagcaac gccattgccg catacgccgc cacagcgaac gccagccagg
    12421 attggcccca ctccgatctt tcggccttcc acgccgcagc cgccagcatg acggccgacg
    12481 ctgaccagta aacaatatcc ccgaaatagg aaccggcgct cgcgaaagcg agcgctaaaa
    12541 agaaaagggg ggccggtagc cccccttcat atcttccgac cagtgtagcc ctggcaggct
    12601 ggcttggtgt caactcaaag cgttgcgggg ttgcttacgg ggtgcaaatt cccggcgtgg
    12661 gcactgagca agacgcgact tcggttccac cgctagcagt ggcggtaaag ttgcacccac
    12721 ccgtgtgagc aatcgttaca ccggtcgacg ctccgtttac gcccgcaacg atgctggcag
    12781 acggaaccgc ggtacgcgtc gaggcgtaaa aaatgacggc ctgcgactgc accgcgccgc
    12841 aaccggcatc ggcgaccgcc tggcgcgcac tgctggaaat gtctacgaat tggggaacag
    12901 cgacggccgc cagtatgccg agaatcacga tcactaccac gagctcgatc agggtaaagc
    12961 ccgcttgtaa tgccttgcgc atgatgtttc ttcctctaat gttcttcagt tcggtcggac
    13021 cggaacccgg tgcccaggtg cccgcccccc ttttatctat gactcccatg ctaccggcgg
    13081 ctttgtaagc cttcaagacg attttgtcaa tatgagtcaa ttggttcccg aatattacta
    13141 agaaagcaag tgagattcct cacggtgccg gctcccagcg gacagacccg acaggcttca
    13201 gcgacaccac cgtgaacccg gtgtccggtg cccggaacac ctgcagcctg aaacgcagat
    13261 gcgacgccgg ctgttcctcc agtcccacca ggtagcgctt gaggcgtgga aggtagacaa
    13321 gctcgttgcg caggcgatca aagagccagc ttccgccctg cgcttcggac ggcaagccca
    13381 cgaactcccc caggtagttc gtcgatcgca tggccgcgga cacaaatggg cttcgcttcg
    13441 gcagcgcctc gatcgcctcc tgctcacccc gcaggctgag atacgccacg cgcgcatata
    13501 gcgccgattg caggcggcta atcgtcgcct ccatggcaga tttttcggcg acttccgcgt
    13561 aagtgagcag tcgctccaga agtaccgtcg caagtatccc gacgatgatc aagtacagcc
    13621 cggactgcag gtaattgcgc tgccggctgg cggggatcac ttctgcagca gcacgttgcc
    13681 gaggccccag atcggcagga acacgcccag cgccaggatc agaaccatca cgcccagcgc
    13741 tatggtgagg atgggctcga tgttcgccgc aagattcttg atctcgtaat cgacctcgcg
    13801 ctcgtacatg tcggcgacct cgtccatcag atcgtcgagt tcgccggtct cttcgccgac
    13861 cgagatcatc tccaggacga cgggcgtgaa gatgccgctt gccgtcgccg tgcgcagcac
    13921 gctctcaccc cgctcaaccc catcccgcat ctggccgatc cgaagcgcca tgtgggcgtt
    13981 gtttaccacc tctcctacca ccgacatcgc ctgcacgatc ggcaccccgg cgcgcactgc
    14041 gagcgacatg ccccgggcaa agcgcgcgag cgtcgcgcgc agaatgatgg tgcccgcgag
    14101 cggcagcctg agcttccagc gatcccactg atggcgcccc tgctccgtgc cgacgtagat
    14161 ctgcgcgccg aagatggcgg cgaccagcgc gcccagcatc gcaggccaat acgcgaggaa
    14221 gaaattggaa gtgccgatca gcagctgcgt cagaagcggc agcggcacct tctgcgccgc
    14281 gaagatcttg gcgaacgccg ggatgacgaa gaagttcacc accgccaccg ccaccgcgat
    14341 cacgaccagc acgatgatcg ggtagcggac cgccgacttg atgcggtcgc ggatttcctt
    14401 gtcgaactcc atgtagccga acatgcggtt gaacgactgc tcgagctcgc cggtcgcctc
    14461 gccaatgcgg atcacgctga cgtaaaacgg gctgaacacc ttgggatggc cgcgcatcgc
    14521 cgccgagagc tcgcggccgg catccaagct ggtacgcaaa tcgccgatca cttcggctag
    14581 cgatgaattg cgtgtggact gttgcagccc tgtcagggcg cgcatgattg gcacgcccgc
    14641 cttgagcagg gcgtacattt gccgcgagaa cagcatgacg tccacgtcgg tgatcggcgg
    14701 cgcgctgaag cgccgccacc actcgggcgc ctcgatggcg gtgggctcgg cgacgggctg
    14761 gatgtccacc ggggtgacac cgatagccag gagctggtcg gccaccgcgc cggaatccgg
    14821 cccttccagc ctgccggtca cgaggccacc gcgcgtatcc cgcccgcgat aggcgtagtg
    14881 cggcacccta ctcctccacc tgggtcgcca ccttcagcac ttccgcgacc gtggtgcggc
    14941 ccttgagcac cagctcgagc gcccggtcgg tgagccgcgc gccctgcatt tgcggccgcg
    15001 ccacctctcc gaacatgttg gggtcgcgag ccaccacggc acgcgccagc tcaggggtca
    15061 tctccagcat ctcgtacacg ccgatgcgac ccgcgaaccc cgtgccgttg cagtgcgagc
    15121 agccgcgtcc gtggaagtac ttgtggctgg cgtagtccgc gccgacctcg gccgccagcc
    15181 agcgcgactc gttggcgtcc ggctggtact cggtgttgca ggtttcgcag ttcaggcgca
    15241 ccaaccgctg cgccagcacc gcatgcaccg aggtggccac catgtaggcc ggcgccccca
    15301 tgtcgatcaa gcggatcggg gtgctgaccg agtccttcgt gtgcaaggtg gaaaacacca
    15361 agtggccagt aagcgccgcc cgaagcgcga tctgcgccgt atcctcgtcg cggatctcac
    15421 cgaccagaat gatgtccgga tcctggcgca gcgccgagcg cagcacgcgg gcgaacgtga
    15481 gatcgatttt ctcattcacc tgcacctgat tgatccctgc gaggcggtat tcgaccgggt
    15541 cctcgacggt gaagatcttg cgctccggcg tattcacctc ggacaccgcc gagtacagcg
    15601 tggtggtctt gccgctgccc gtcggcccgg tgaccagcac catgccggcg gtcttccgga
    15661 tgatctcgcg gaagcgctgc agcatctgcg gcggcatgcc caggctgtcg agcgactggc
    15721 gcgtggcgcg ccgattcaac aggcgcagca ccaccgactc gccatactgc gtcggcatgg
    15781 tcgacatgcg cacgtccacc ttctggtcgc gcgcaatgac gttgaggcgc ccgtcctggg
    15841 gcaggcgctt ttccgagatg tccaggcccg ccatcagctt caggcgcagt gccagtgccg
    15901 gcgcgatctt ggggtcggcc tgcgtctgcg ggttgagcgc gccatcgatg cgaaagcgga
    15961 tctgcagcgc ggtttcctgc ggctcgatgt gcacgtcgga ggcgttcacc tggatcgcgt
    16021 cttcgaacac gctctgcagc agcttgacga cgggcgcgtc ctcggcgccc gcgccaagcg
    16081 cgccgaagtc gacataggaa tcgccgacgt cgcgctccag ctcctttgcc aatcccgaga
    16141 tctcctcggt gcggcggtac acccggtcca ccgtctgcaa cagcgccgct tcccccacca
    16201 ccgccacctc gaggtccttc tgcagaatcc ggcccagctc gtcgtaggcg aacagatcgg
    16261 tcgggtcggc catgccgacg agcacactcg cgcgccggtc ctcgagcacg atggcgcgaa
    16321 agcgccgcgc ctgcgcttcg ggcaggcgcc gcaccacatc ctgattcagg ttgtagaact
    16381 tgagattgac gtactggatg ttcagctgcc gcgagatcgc ctcgcagatc tgctcgtcgt
    16441 tgacgaagcc cttctcgaca agcacgcgcc cgagccggcg gccgctcttt ttctgctgct
    16501 cgagcgcgac cctcaactgc tcctgcgaaa tcgccttctg tgcgacgagg acgtcgccga
    16561 ggcggatctt ttccgggcgt cccatggcgg tgctccgtac gagggcggcg cggcgcacat
    16621 tatccgcttt tacgtcctgc ggcaaacgac taccattgcc cggagctgac aaaccctcaa
    16681 gcgagtcccg gcgcgatgaa aaccttcgcc tcccaggccg atctcaagcc caagaagatc
    16741 agcttccgga agctttcgag aaacgcctac gcgttcaccg cagaaggcga cccgaacact
    16801 ggtgtgatcg tcggcgacga cggcgtgatg gtcatcgata cacaagcgac gccgctgatg
    16861 gcacgggaag tgatccgccg tgtgcgccgc gtcactccta agcccatcaa atacgtggtg
    16921 ctgacgcatt accacgcggt acgcgtgctc ggcgcctcgg cctaccgcgc gcaacagatc
    16981 atcgccagcc ggcgcacgct cgggctgatc gaggagcgcg gccagcagga catggcgagc
    17041 gagatcgggc gctttccgcg ccttttccgt ggcgccaagt ccatcccggg cctgacctgg
    17101 ccgacactcg tgttcgatag cgcgctcacc gtgatgatgg gcaggctcga ggtgcgcatc
    17161 atgcacgtcg gccccggcca taccggcggg gacgccatcg tgtggctgcc gtcgcagaag
    17221 gtgttgttct cgggggatct ggtcgagtac aacgccggca tctacacggg tgacgcgcac
    17281 ctggaggcgt ggccggctac tctggacaag ctgcgcgcgc tcaagccgcg tgcgctggtg
    17341 cccggccgcg gccccgcgct cacgacccca gcgcagtgcc agaaggccat cggctacacc
    17401 caggacttcg tgcgcggcct gtacgcggcc gcgaagcgcg gcgcacgcgc tcgccagtcg
    17461 ctcaggcagg tgtatctggc aaccaagaag aagatggatc cggtctacgg cagcttcccg
    17521 atctacgagc actgcatgcc gttcgacgtc tcgcgcgcct acgacgaggc gcgcggcatc
    17581 aagcatccgc gcatctggac ggcgcggcgc gaccgggaga tgtggaagtc gatcgcgtga
    17641 ccgcaagggg ctaggcgcag ccccgccgcg taggaggagg gaggagaata ccgcggcggg
    17701 gcctgcctaa ccgggttgcg ctagctgtgg tgcagcctcg cgctcgtgca gtccgcggcc
    17761 gtcgcgcgca tgccacggcc caggaaggtg accttcgccc acgcggcggc aaagaagcgg
    17821 gcaattcccc cgcccagcac gcgggcgcgc tcttcacgcg cgcggcgctc gatgtcgccc
    17881 cattccgaaa acgcgagtgg cagccggaag ttggccctca cgtccttttc gatctgctcg
    17941 atttcatatg cattcatcat ccgttccctt tttcgctgtg gatactgcgg tgcagcaaat
    18001 atagggcttt gcaaaatggt ttagaatagc ggcggtagac aaaagtcttt tgcgcaaata
    18061 gcaaagatat gcacaacgta gttcacggca tgctggtttt ttccaaggtc gtggcggccg
    18121 gcagcttgtc ggcagcggcg cgcgagctcg gcgtgtcccc cgcggtggtg agccgcaccc
    18181 ttgccgcgct cgagactcgt ctcggcgtgc gcctcgtgaa ccgcaccacg cgcagcttgc
    18241 atctcacgga cgaaggggca agctactacg agacctcgca gcgcatcttg gccgagatcg
    18301 acgaagccga cgccgcggtg acctcgcacc gcgtcgagcc gcagggcgtg ctgcgcgtcg
    18361 ccctgcccgc ctcgttcggc caccagcaca tcgcgccgct cgtgccgcgc ttcgccgagc
    18421 gctacccgaa ggtggagctc gcgctcagcc tgtcggaccg ctcgatcaac ctgatcgaag
    18481 agggattcga tctggccgtg cgcatcgcgg agctgaagga ttcgtcgctc gccgcgcgca
    18541 agcttgcgcc gaaccggcgc gtggtgtgcg ccagtcccgc ctacctgcgc gcgcatggcg
    18601 agccgaagac cccgcaggac ctcgccaagc acaactgcct cgtggcgagc tgggagcacg
    18661 gcttcgctat gacatgggag tacaagagcc cgaccggcaa gcgcggcacg gtgcgcgtca
    18721 ccgggcgcta cgcctgcgac aactgggaag tgctgcgtga ctgggcggtg gccggtctcg
    18781 gcgtcgcgct gaaatcgacc tgggacgttc gcaggcagct cgaggaggga tcactggtgc
    18841 cgctgctgtc gggctacgat ttcggcaccg acgtcggcat ctacgccatg tatccgcacc
    18901 gccggcatct gccggcgaag acgcgcgcgt tcatcgattt cctcgctgaa tccttcgggc
    18961 ccgaacccta ttgggaccgg cccgcgggcg ccgcgccggc gacgccgccg gcaccgcgaa
    19021 tcaagtcgcg cgccgctgtc taaggctatg gtagaaccac agtagccatg gaggcctatc
    19081 gatgaacgcg ccggtcgatg cccgcaagct cgccaaggtt tctctcgacg acaagtacac
    19141 gctcgggaac gggcgcgtgt tcctcaccgg cacgcaggcg ctcatccggc tgctcatgct
    19201 gcagcgcgag cgcgaccagc gcgccggcct gaataccgcc ggcttcgtgt cgggataccg
    19261 cggttcgccg ctgggcgggc tcgatcagtc gctgtggcgc gcgcagaagc acctcgaaga
    19321 tcatcacatc aggttccagc caggcgtcaa cgaggacctc gccgcgacgg cgctgtgggg
    19381 cacgcagcag gtgggcatgt tccccggcgc caagtacgac ggcgtgttcg gcatgtggta
    19441 cggcaagggc cccggcgtgg atcgctgcgg cgacgtattc aagcacgcca actcggccgg
    19501 cacgtcgaag cacggtggcg tgctcgtgct cgcgggcgac gaccatgccg cgaagtcctc
    19561 gacgctcgcg caccagagcg agcacatcct gaaagcctgc tgcatcccgg tcctcaatcc
    19621 gtcgcacgtg caggactacc tggatttcgg cctgcacggc tatgccatga gccgctactc
    19681 gggctgctgg atcggcttca agtgcgtcac cgacgtcgtg gaatcgggcg cctcggtgat
    19741 cgtggacccc aagcgcgtgc aggtgaagct gccgaccgac ttcgccatgc cccagggcgg
    19801 cctcaacatc cgctggcccg acggcttcct cgagcaggag acgcgccttc tggactggaa
    19861 ggtttatgcc gcccttgcct acgtgcgcgc caacggcctc gatcggattg tgtgggatgc
    19921 gcccgaggcg cgcctcggca tcatcaccac gggcaagagc ttcggcgaca cgatgcaagc
    19981 gctcgccgat ctcggcatcg acgagcgcgt ggcgcgggac atcgggctac gcgtgtacaa
    20041 ggtggccatg tcctggccgc tcgagcccca gggcgcgagg cgctttgccc ggggcttgga
    20101 ggaaatcctg gtggtcgagg aaaagcgcca gcttatcgag taccagatca aggaagagct
    20161 gtacacgtgg gaggcaggca agcgcgcgcc gcgcgtggta ggcaagttcg acgacaacgg
    20221 cgagtggagc cgcgccgaag gccagcccgc gggcacctgg ctgctccctg cgcactacga
    20281 gcacaccccg gccatcgtgg ctcgcgccat cgccaagcgc ctggaaaagc ttggcctcgc
    20341 cgagcggctc ggcgcgcagt tcaaggagcg gctcgcgttc ctcgacttca aggaaaaggc
    20401 gctggccaag ccgcgcgtca ccgcggtgcg ccagccctac ttctgctcgg gatgcccgca
    20461 caacacgtca acgcgcgtgc ccgagggcag ccgcgccacg gcgggcatcg gctgccactt
    20521 catggcggtt tggatgaacc gcaatacctc caccttcacg cacatgggcg ccgaaggcgc
    20581 gccctggatc ggccaggcgc cgttcaccgc ggaaaagcac atcttcgcca acctcggcga
    20641 cggtacctac ttccattcgg gcctgctcgc catccgcgcc gcggtggccg cgaaggtcaa
    20701 catgacctac aagatcctct acaacgacgc cgtggcgatg accggcggcc agcaccacga
    20761 cggcccgctc gatccggcga tgatctcgcg ccagattgcg gccgaagggg tgaagcccat
    20821 cgtgctggtg accgacgagc cggaaaagta cccggcgggc acgaactggg cgccgggcgt
    20881 gcagatccgc caccgcgacg agctcgatgc cgtgcagcgc atgctgcgcg agcagcccgg
    20941 cgtgtcggcg ctgatctacg accagacctg cgcctccgag aagcgccggc gccgcaagcg
    21001 cggcgcgtac cccgatccgg ccaagcgcgc ggtgatcaac gagatggtgt gcgaaggctg
    21061 cggcgactgc agcgccaagt cgaattgcct ctcggtggaa cccctcgaga cggagttcgg
    21121 gcgcaagcgc accatcaatc agtccacctg taacaaggac ttctcctgcg tgaagggctt
    21181 ctgcccgagc ttcgtcaccg tcgaaggcgg gcaactgcga aaaggcaaga aaggctcgcg
    21241 cgctgcgggc gaggacttcc cggcgctccc cgaggccgcg ccggcgccgc tggacgagcc
    21301 ctgcggcgtg ctcgtgaccg gcatcggcgg cacgggcgtc atcaccatcg gccagatcct
    21361 cggcatggcg gcgcacctcg aaggcaaagg cgtcgcggtg ctcgacatgt cgggcctggc
    21421 gcagaagtac ggcgcggtga tgtcgcacgt gcggatcgcc tcgagcgcgg atcaggtgca
    21481 cgtggcacgc ctgggcacgg gcgatgcgcg cctcgtcatc ggctgcgacc tggtggtaag
    21541 cgcgagcgcc gatgcgctcg acaggatgag cgccgggcgc acccgtgccg tcgtcaacgg
    21601 caccacgtcg ccgacggcgg cattcgtcag caacccggac tggcagctgc cgggctcgaa
    21661 cctgcagcac gacatcgccg aggcctgcgg cggccaaggc gtcgaattcg tgcccgcggc
    21721 cgaggtcgcc acggcgctga tgggcgattc catcgccacc aacatgttca tgctcggcta
    21781 cgcctggcag aagggctggg tgccgcttgg gcgcgacgcc gtcgagcgcg ccatcgagct
    21841 gaacggcgtg gcggtcgagt tcaacaagca aagcttcctg tggggccggc gcgcggcggt
    21901 ggatctcgag cgcgtacggc gcatcgccat gccggcggac gtcattccca tcgaccagca
    21961 tgtttcgcgc aatctgggcg agctggtcga gcggcgcgtg cagctgctca ccgcgtacca
    22021 gaacgccgcg tatgccgcgc gctaccgcgc gctcgtcgag cgggtgcgcg cggccgagca
    22081 ggacgcggtg ggtggcacga agctcgccga agccgtggca cgctactacg cgaagctgct
    22141 ggcctacaaa gacgagtacg aggtggcgcg gctctacgcc acgggtgaat tcatgaagaa
    22201 gatcgacggc atgttcgagg gcggctacaa ggtgaacttc cacctggcgc cgccgcttct
    22261 cgccaggccc gatccgctca ccggcgagcc gcgcaagatg cagttcgggc cgtggatgct
    22321 gcgcgcattc ggcctgctgg cgagattgaa gggcctgcgc ggcacgccgc tcgatgtctt
    22381 cggtcgcacg gccgagcggc gcatggaacg cgcgctgatc ggcgagtacg agaacaccgt
    22441 gcagacgttg ctaggcggcc tctcccgcga ccatcacgcg ctcgccgtgg agatcgcgtc
    22501 cctgcccgag agcattcgcg gcttcggcca catcaaggcg aagagcgtag aggcggcgcg
    22561 caggaaacag gcggagttga tggcacgcta tcgcaccgcg ccggtcaagg cggcggcata
    22621 gcgtaaatgg ggacagacct ccttttccac gaacggcgag gaaaatgagg tctgtcccca
    22681 tttacaggcc cagcgcctgc cacatcgcgt cgaccttctg cttcacctcg gcgtccatgg
    22741 cgatcgggcg gccccaggtg cgtgaagtct ctgccggcca tttgccggtc gcgtcgatgc
    22801 ccatcttcga gcccagtccc gccgccgggc tcgcgaagtc gaggtaatcg atcggcgtgt
    22861 tctcggcgat cagcgtgtca cgcgccgggt cgacgcgcgt gctgagcgcc cacaccacct
    22921 ccttccagtc gcgcacgtcg atgtcgtcgt ccacgacgac gatgaacttg gtgtacatga
    22981 actgccgcag cacgctccag atgccgaaca tcacccgctt ggcgtgcccc ggatactgct
    23041 tcttcaggct caccacggcg agccggtagg agcagccctc gggcggcagg tagaagtcga
    23101 cgatctcggg aaactgcttc gccagcagcg gcacgaagat ctcgttcaac gccacgccga
    23161 gcatcgccgg ctcgtccggc ggcttgccgg tgtaggtcga gtggtagatc ggatcccggc
    23221 gcagcgtgat gcgctccacc gtgagcaccg ggaaccgctc ggcctcgttg taataacccg
    23281 tatggtcgcc gtgcgggcct tcgagcgcgg tgtcgccggg gtggatcgcc ccctcgagca
    23341 cgatctcggc gcgcgcgggc acctgcaggt cgctgcccag gcacttcgcg agctccgtgc
    23401 gcgcaccgcg cagcaggccc gcgaactggt actcgccgag cgcgtccgga accggcgtca
    23461 ccgcggccag cgtggtcgcc ggatcggcgc ccagtgcgac cgcgaccggg aacggcgcgc
    23521 cgggctgcgc cagctggtgg tcgcgaaagt cgagcgcgcc gccgcgatgc gcgagccagc
    23581 gcatgatgac gcggttcgcg ccgatcacct gctgtcggta gatgccgaga ttctggcgcg
    23641 gcttcagcgg cccgcgcgtg acggtgagcc cccaggtgat gagcgggcct gcatcccccg
    23701 gccagcaggt ctgcaccggc aggcgcgcga gatcgacgtc cttgccttcc cagaccgtct
    23761 cttggcaggg cgccgagctc accgtcttcg gcgccatggc gaacaccttg ccggcgagcc
    23821 gcgcgagctg gggcagtttg gtcaccgcat cgcgcagccc cttgggcgga tcgggctcgc
    23881 gcagcgcggc gagcagcctg ccgatgtcac gcaaggccgg gcgcggatcc gttccggcgt
    23941 cgacgcccat ggccagcgcg atgcgctcga cggtgccgaa caggttcccg agcaccggca
    24001 gcgcgaagcc cgcaggctgc tcgaacagca gcgccggccc gcccgcgtgc agaacgcggt
    24061 cgcagatctc ggtcatctcg aggcgcggcg atacgggtgc ggcgatgcgc tttagcccgc
    24121 ccagcgcctc aagtccgccg aggaactcgc gcagatctgc gtagtgcatg cttcagcgac
    24181 cgctttgcgc cacgcgctcg gccagcaact cggcgaaatc caccagcatg cggttctgcc
    24241 cgcgtaccgc gtaggcgcga tcggcggcgg gcagcgagag tacgagcgta gtggtgccga
    24301 tccagccaaa agcagcgagc gcacccccgc ccagcaggca gcccagggct accgcgggca
    24361 gcaggctcgc gatcgcgccg agcacgagca aggtgccccg cagcaactgg gccaccgagt
    24421 gattgccact cacttcggcg gccgcctcgg cagcggcgcc tgaaagcggg ccggccacgg
    24481 cgaacaggac aagcgcgagc agcgcgagcg ccacgccccc agctatgcgc cgcacgtcgc
    24541 gctcgtagcc gacgcgcacc gcggcgatgg cgccgagcgc catgctgtcc atctggccga
    24601 cgccatggtg caagaggcgc gtgccgtaga gcgagagttg cgtgccgcgc aacggcccgg
    24661 tggaaaattc gtagttcgcg atcggcgttt cgagagccat cggcgaagct tagcgcaggc
    24721 cgtagtgcgc cgcgatgccg gcgaacaccg cagcgcccac ccagttgttg tgccggaacg
    24781 ccttgaagca gcccgtgcgg gagcgatcac gaatcagccg gtagtggtag gccatcaggg
    24841 ctgcggcgag cgcgagcccc gcgtagtacg gccagccgag gcgcgccatg acgccgacgg
    24901 cgccgagcag cgccagcatc ccggcatagg cggccatcac gcccgccacg tcgtaacggc
    24961 ccagcgtcag ggcggaggtg cgcacgccga tcttcgcatc gtcgtcgcgg tccaccatgg
    25021 cgtactccgt gtcataggcg aaggcgtagc acacgttggc ggcgagcagc acccagcact
    25081 ccgccggcag ccggctctgc gcggctgcgt aggccatcgg gatgccgaag ccgaaagcaa
    25141 ccccgaggta gagctgcggc aaggcgaaaa agcgcttggt gagcggatag gtaatggcga
    25201 tgccgagggc gacgaacgac aactgcagcg cgagccggtt cagcatcagc accaagccga
    25261 aggccgccgc ggcgagcagt gccgccagca ccagggcttc tgccggccgg atctcgcccg
    25321 cggcgagcgg ccgaacacgc gtgcgctcga catggcggtc gaatccgcgg tcgaagaaat
    25381 cgttgaccgc gcagcccgcc gagcgcatga gcaacgtccc gacgcagaag atggcaatca
    25441 gttccaccgg cggcaggccg cgcgcggcga gccacagcgc ccacagcgtc ggccacagca
    25501 gcagcagggt gccgatcggc ttgtcgagcc gcagcagccg ctcgtaggca tccaggcgct
    25561 gctcgatggc ggtccaggac atgtgagcgc gagtctagac cttgccgaag tcggcccgca
    25621 ggccgagcca gcgatcgata tggcggcgcg cgagcccttc gtccgccgcg agcagctcat
    25681 cggcagcggc gcgcgccgct tcgagcacgg gctcgtcggc caccaggtcg gcaaagcgca
    25741 gcagcggcgc cccgctctgc gccacgccga gcagctctcc cggaccgcgc agcaccaggt
    25801 cgcgctcggc gatggcgaag ccgtcggcgt tctcgtagat caccttgagg cgcgcccgcg
    25861 ctccatcgga cagcggctcc tggaacagca ggacgcagtg gctcgcgcgc gcgccgcggc
    25921 cgacgcggcc gcgcagctgg tgcagctgcg ccaggccgaa gcgctcggca tgctcgatga
    25981 ccatcagcga ggcgttcgga acgtccacgc ccacctcgat taccgtggtg cagaccagaa
    26041 gctgcgtggc gccctcggcg aacgcctgca tcgcggcggc cttctccgcc ggcgccatgc
    26101 gcccgtgcac gaggccgatg cgcaggcgcg gcagctcctg gcgcagctcc tcgtaggcct
    26161 cgagcgccgc ctgcaggtcc ccgtccttcg actcctcgat caccgggcag acccagtacg
    26221 cctgctcgcc gtcggcgcag gcagcgcgga tgcgctcgag cacttcggcg cggcgtgcag
    26281 cggcgacgag gcgcgtggtc acgggcttgc gcccgggggg cagctcgtcg atcaccgaca
    26341 cgtcgaggtc cgcgtagtag ctcatcgcga gcgtgcgcgg aatcggcgtc gcgctcatca
    26401 tcagctggtg cgccgccgcc tgctcgctct ttcggcgcag ctgcaggcgc tgcttcacgc
    26461 cgaagcggtg ctgctcgtcg acgatggcga gcgcgagccg cgagaactcg accccctcct
    26521 ggaacagcgc ttgcgtgccg atggcgatgc ttgccgcgcc gctggcgatc gacttgcgcg
    26581 ctcgccggcg ctcggcggcc ggcaagccgc cgtgcagcca cgcaatgctc acgccgagcg
    26641 gtccgagcca ggtggagaac ttgcggtagt gctgctcggc gaggatttcg gtgggcgcca
    26701 tcactgccgc ctgcccgccg gcgtccaccg cggcgaggca gccgatggcc gcgacaatgg
    26761 tcttgccgct gccgacatcg ccctgcagca gccgctgcat gggatgtgcc tgccccaggt
    26821 cgcgcagcag ctcggccatg actcgggctt gcgcgcgcgt cagccggaac ggcaggcggc
    26881 cgaggaagcg cttgagcagc ggcccactcg cgggtaatgc gggcgcgctg cgctccctgc
    26941 gcttgcgccg cgcgagccgc aactgcagct gctgcgcgag cagctcgtcg aacttgacgc
    27001 gctgccaagc cgatgctgtg tccgcgcccg gggccggccc gtgcagcagg cgcacgctcg
    27061 ccgccagcgg cgcgagcccc aggcgctcgc gcagcgacgc gggcaggatc tcctcgatcg
    27121 gctcctccgc cagcgcttcg agcaccagct tgcgcagcgt cgcctggggc aggccggcgg
    27181 tcgtcgggta caccggcgtc agcgtgtcgg cgagcggtgc gtccggcgcg acgatgcggt
    27241 agcggggatg cacgatctcg gcgccgaacc agcccggccg cacctcgccg taggcgcgca
    27301 cgagcaggcc ttcgcgcgcc gcgcgctcga actgcttcac ctggctgccg taaaagttga
    27361 gaaagcgcag caccagcccg tccgcgtgca ctaccagctg gcgcttcggc cggaacgcga
    27421 tctccgcgcg gcgcaccggg gcctgcacca gcagcggcgc gccgggcgcc gcccgctcca
    27481 gcggcgtgag ccgtgtttcg tcctcgtagc gcagcggcag gtgaaacagc aggtcgtggc
    27541 gcgagcggat gccgagcttg gcgagcttgc tcgccgtggc gggcggcagt gcgcgcgtca
    27601 accggcgtgc aggatggcgt cgacttcgac cagcgcgccg cgcggcaggc tcgccacgcc
    27661 gacggccgcg cgcgccgggt agggctgcgg cacgtactgc gccatgatct cgttgacctt
    27721 ggcgaagtgc gcgagatcgg tgaggtacac ggtcatgcgc acgctgtgac cgagcccgag
    27781 ccccgcggcc tgcgcaacgg cggcgagatt ggcgaacacg cggtggatct gcgcgtcgat
    27841 accctcggcg agctgcatgg tggcggggtc gaagccgatc tggccggaca gatataccgt
    27901 gcttccggcg cgcacggcct gggagtaggc gccgatcgcg gcgggcgccg cggcagtctg
    27961 gatcgcggtt ttcatggcgg gagccgggct tagaatgtcc ggtccatctt agccgaaatg
    28021 acgacgccgc tcattgccag cctggaaaag ctgctcggca cgccgcgcga cggcgcgctg
    28081 ctgcgctatt cgctcggcag cgagtgcctg aaagcgggga gcctcgagcg cggcatagag
    28141 cacctgcgcg aggcggtgcg gcgcgacccc cagtactccg cggcgtggaa ggtcctgggc
    28201 cgcgcgctgg ccgctgccga ccgcgtgcaa gaggctcgcg aagcctacgc gcgcggcatc
    28261 gaagcggcgc gcgccaaggg cgaccggcag gcggagaagg agatggcggt gttcgcgcgc
    28321 cggctggagc ggcgcagcgg cgcctaggcg cgcggcaagt gcgcgccggc cacttcggcg
    28381 cagtctggcc gcccgaagta atagccctga gcctggttgc agccgtgcct gcgcaggaac
    28441 gcgacctgct gctcggtctc gacgccctcc gcgatgaccg tgaagcgcag gttctgcgcc
    28501 atgtcgatga ccgcgcgcac gatcgaggcc gcctcggcgc tcgcgccggc gtcgcgtacg
    28561 aagctgcggt cgatcttgag cgctgacagt ggcagtcgcg tgaggtagga caggctcgag
    28621 tagcccgtgc cgaagtcgtc gatcgagatg cacaggccgg ccgcgcgcag gttgtgcatc
    28681 acgcgcaccg cctgctccgg atcctccatc aggcagctct ccgtgatctc cagctcgagc
    28741 agcccggcct ccacgtcatg gcgcgcgagg aggtcgcgaa tgatgcggtc caggtcgctg
    28801 cggcgaaact gcttgggcga cagattgacc gccaccggca ccacgctgcg cccctcgtcg
    28861 cgccagcggc gcagctgcgc gcaggccttg ttgatcaccc attcgccgac cggcaggatc
    28921 aggcccgaat cctccagcgc cggaatgaac tcgcccggtg acaccacgcc gcgctgcggg
    28981 tggtcccagc gcagcagcgc ctcaaggcca cgcaggtggc tgtcggcgag gtcgaccttg
    29041 ggctggtagt gcagcgtgaa ctcctcgcgc tcgatggcac ggcgcaggtc cgtgttcagc
    29101 tgcaccttgg cgacggtgcg ctggttcatc tcggtggtga agaagcggta gcagttgcgt
    29161 gccgattcct tcgcccggta catggcgatg tcggcgttct tgagcagcgt ctcggcgtcg
    29221 tcgccgtcct cgggaaaggc ggcgatgccg atcgaggcgc tgatgtaggc ctcgttgccg
    29281 ccaaggtcga acggctgcgc cagcgcatcg aggacctttt gcgtcaccag cgcggcatcg
    29341 tcaggccggg ccaggtcggc gagcacgatc gcgaactcgt cgccgctgat gcggcccacc
    29401 gtgtcgcccg ggcggacgca ctcgagcagg cgctcgccca cctggcggat gagcgcgtca
    29461 ccctggtggt ggcccagcgt gtcgttgatc agcttgaagc ggtccaggtc cacgaacagc
    29521 gcgcccgcgt gccagcgctt gcgccgcgac tgcacgatgg tctgcgccag ccggtcctgc
    29581 agcagggtgc ggttcggcag cccggtcagc gcatcgaact gcgcgagatg cgcgaggcgc
    29641 tgttccgcct tctgccgctg cagcgccgtg gacagcacat ggccgacgga ctccgcgaag
    29701 cgcaggtcct cggtgctgaa ggcgcgctcc tccaacgcgt acacgcacag catggcgatc
    29761 gggctcgcgt cgtcgcggac cggaacgtac atggcgctgc ccatcgtgcg cacccaggcg
    29821 tgccagggac ggtcgagcgg ccgcgaggac aagtacttcc tgccggcgat gcgcggggtg
    29881 ttctcgcgca ggatctccgg ccacacgctg tcggccgcca tcggcccgcg cacgccgagc
    29941 atgccgtgcg gcccctcgcc ctgcgccgcg cgcaccaggt actcgccctt cgccgtgaac
    30001 atctccagca cgcaggccgc gtccacgccc tcgcagcgca gcgctcgcgc ggcctcggcg
    30061 tacagctcct cggtcacgcg ccgcccgagc gcgaactgcc cgaatctcgc gatcgccgcc
    30121 tggcgctcgg cgtgcacgcg cacgtgctgc tcggcgtgct tgcgctcgct gatgtccatc
    30181 atcgcgccga tcatgcgcac gggctggccg cgctcgtctt gcatcagcag gccgcgatcg
    30241 aatacgtcga cataaccgcc gtccttgcac aggaaccggt actcgccgga ccaggccttg
    30301 gcgccgccgc gaatcgccgt gtcgacctgc gacttcacgc gcgccgcgtc gtccggatgg
    30361 atacgcgaaa tccagaagtc ggcgtaggcg ccgacttcct gcggcgcgta gccgaacagc
    30421 ctctggaagt tctcgttcca ccagacctgg ttggtgacca ggttccaatc ccagaccacg
    30481 tcgttggtgg cgcgcgacac catctcgaag cgctcatggc tcgcgcgcag ccgctcctcg
    30541 ctgcgcaggc gctcggtcag gtcgcgggca atgccgatga tgtacgtgcc ctcgcccgtg
    30601 cgcagaacac ggcgcgtgac ctcgaccggc acgatggagc cgtccttgcg ccggtggcgc
    30661 atctgcagca gctcggagcc atgcgggtcc tgcagcagct tgtcgtacgc cgcgcgcatg
    30721 tcctcggcac tgcggtccgg gaacactgcc gtgaccggcc gcccgagcaa ttcatggcgc
    30781 tcatagccga ggcttctgag cgcggcatcg ttgatatcga tgatgcacag agtgtccgca
    30841 tcgatcagcg agatcgcgtc catggaaatg tccagcgcgg cgcgaaagcg ccgcaaggcc
    30901 atcgtcgcct gcatggtctc cgtgacgtcc ctggcaatgc cgcggtagcc acggaacacg
    30961 ccgtcctcgc cgaggatcgg ctcgcccgag atgctcacgt agtgcgtccg gccgtcgttg
    31021 cccttgcgct tgagcatgaa gtcgcggaac ggctggcgcg cctcgagttg cgccttgtgc
    31081 gcctcccatc cgccttccag cggctcggtg cccggcagct cccagcggcg cagtccgtcg
    31141 tgctgagcgg cggtgatgcc gccgcgcgcc ttgtcctggc cgccggtgcc gacgaagcgc
    31201 aactctgcgt cctgctccca gtaccagtcg gccgagagtt cggtcaggct gcggaagcgc
    31261 tcctcgcttt cgcgcagcgc gcgctctgcc tccaggcgct tgctgatgtc gcgcgagatg
    31321 gcgacgatga gcgcgccgtt ctccgactgc agcacgcggc gcgtcgattc gaactggatc
    31381 gtcgagccgt ccttgcggcg gtagaagctg ttcatgccgt ggatcgtgct gggatcggcg
    31441 acgaaccccg cgtattccgc ttgcaggact tcgcgcggcg ccgggagaag atcgtgcggg
    31501 cccatcgcga gcatctcttc gcgcgagtag ccgagcgtgc ggcaagccgt ctcgttgacg
    31561 tcgacgtagc gcatcgcctt gtggtcgatc agcaggacca tgtcggcgga ggcgtccatg
    31621 ccggcgcgga agcgccgcat cgcttcttcg ttccgcttgc gcagcaggaa ctggccgatc
    31681 tggtcaccaa tcacgccggc ggcggcgagc agccgctcgt cgggcttgcg gacttcctcg
    31741 ctcgcgatgg tgatcacccc gaggaccttg cccgccccct tgacggggaa catgaacgtg
    31801 ccgcgcagcg gcgtgcgcca cagcgtgtgc ttggccgcgc gcgcatcctt gctcgcgtcg
    31861 gcgacccaga tcggctcgcc cgactgccac acccggcccg cgatgccctg cccgggctga
    31921 aacaccacgt cgcgcagcgc ggaggtgaag gcgtccgcgc ccccgccttc cctgctccag
    31981 tgcgccgcaa accgcatcag tccggcctcg tcgtccacct gccagaagcg gccgtgatgc
    32041 cactggtgca gctcgcagat cgcgcggatc acggcctgca cgccggcgag cgcgctttcc
    32101 gcctcggcca gatggcggtt gacggcatgt tccagctcga gcgtgcgcag ggccgacttg
    32161 cgcaccgtaa tgtcggtgcc cacgccacgg tagccccgga acgcgccctg ctcgtcgaac
    32221 accggttcgc cgctgatcga gaagtcgcgc gacccgccgt cgggcgtcgg ccggcagaac
    32281 tcgaaaccct ggaacggctg gtgcgcatcg agcagcgcct tgtgcgccct ccagcctgcc
    32341 acgtccgggg cgagggccgg caactcccag cgccgcttgc cgatgccggg gtcggtgagc
    32401 tgcgcgctcg cccgcccgcc gccctcgcgc ttggttgtcc ggtgctcggc atcggtctcc
    32461 cagtagaagt ccgacgagag cgccaccagg ctgcggaagc gctgttcgct ttccttcacg
    32521 gcggcctgag cggcgctgcg ctggcgctcg tgctccaggt tgtccagcgc gaacgaaacg
    32581 ttctgcgcca tgcgctctgc gagccccacc agctccggat cgaaccaacc ggtctcggcg
    32641 gcgtacagaa gaatcacgcc gatgacttcg ccgccgcggc gcaacggcac gcacatcgtg
    32701 gccgcgatct ccgcctcccg cgccggggca tgccagtgcg caagcgtcgg ctcggcgaga
    32761 aattcgttgt gcaccagcgt gcggttctcg agcaccgccg tcaccgccgg gccgcgcgat
    32821 tcgggccgcg cgcgatcgat cgatacgcgg actttctcga gatacgacat cgggccgccg
    32881 gcatgcgccg ccagctccat ccagtgcgtg ttgcggtcca ccagccgcac tgtcgcgagc
    32941 ttgaggcccg cgtgctccac cagcagttcg cagatgcgcc ggtagagtga cggctcgtcc
    33001 tcgctcttgg cgatcgcctc gttggcgttg ctcagcgccg cgtacatgcg gcgcacgcgc
    33061 tcggccccga gctcggcgcg ttttcgcgca gtgatgtccc gcgtgatgct cagggcgagc
    33121 gtcttccccg tcgccgcgtc ctggaggggc accgcgtgca tttccatcca gcggcgcgtg
    33181 cccttcaatc ccacgatctc gaactcgagc tgaccgcgct cgccgcggca gacgcggtcc
    33241 gtgaggtctt tgtagcgctg ccgatacgcc ggcgccacca gcgcgtacac gcactgcccg
    33301 gccaccgcgg ccaggctctc ggcctcgatc atcgcgaggc cggcgggatt catctccagc
    33361 acggtgccct cggcgtccag cagcttgacg cactcgggct ctgtgccgac gatggcgcgc
    33421 agccggctct cgctcgcgcg caggcgctcc tcggcgcgta ccgcgtcggt cacgtcttcg
    33481 aaagccgcga tgtcgtactt cggcgcgccc tgcgcgtcgc gctcgagcga caccgtcagc
    33541 tggacccaga gcgtggcgcc gtccttgcgc aagtagcgct tgcgcgtcgt gaattgaggg
    33601 accacgcccg cgcgcagctg ccggtgcagc gcgtcggcgg cggcgctgtc ctcggggtgc
    33661 gagagcccgc gcaccggacg cccgatcagc tcctccgacg tatagccgaa catggtgcac
    33721 aggcgccggt tgacgagccg gaagcgcccc tcgagatcca cgtgcgcgag accgacgggc
    33781 gagttctcga acgtggcggc aaagcgctcg cgatgctctt cttcctgcgt cacgtcgcga
    33841 tagctgcaga cgatggcggc gacgtcggca tcgcccaggc ggttggcgag cgagctctcg
    33901 aggacgcgct gcccgccgtt cttgtgcaag aagcgcacgc gcagcgtgac gaagcttccc
    33961 ggctgggcgc tcaacgcacc aaacgcctgc tgcacggcgg gccggtcgtc gggcaccacg
    34021 aagctcaggc ccgcgcggcc cagaatctcc gccgtgttga agccgagcac gcggcgcatg
    34081 gcggcactcg cgtactcgat gcggcccttg gcatcgagca gcaccagtgc ttcggagctg
    34141 gactcgagca gcgcgcggta gcgtccttcg ctggcgccct cgcgccccgc ctgcgggtgc
    34201 cggatcgcga ggcgcagcac gcgccgcagc gtagccgcgt cgagctcatc cttcgggatc
    34261 acatcgtagg cgcgacgctt gagtgcctcg tccaccagcg acggatggtc gttgccggag
    34321 acgacgatga ccggcccccg tgccacggcg cgcacgcgct ccagcgtctg caggccctga
    34381 ctgtcgggca ggtcgaggtc gagcaacgtg atgcgcacgt cctgcgactg cagcagcgcc
    34441 agcccttcgg cgaggctgcg ggcatgcagg atctccgcgc cgccgagccg ggcgctcgcc
    34501 aggtgcgact gcagaagctc ggcgtcgttc gcgtcgtcct ccaccatgag caggcgcagc
    34561 gccgccggac gcaggtcctc ggccttcagc gcgctgacct gggtcatgcc gcgaatctgg
    34621 cggactcgag ccagcgactg ttctgctcgg cgagcgcagg acacgcgaag tggtagccct
    34681 gcgcctggtc gcattcgtgc ttgcgcagga aagccacctg ctgcggcgtt tccacgcctt
    34741 cggcgatcac ggtgaagcgc aggttgtgcg ccatgtcgat caccgcgcgc acgatcgagg
    34801 cggcttccgc gttagtgacc gcgtcgcgca cgaagctgcg gtcgatcttg agcgcggtga
    34861 tcggcaggcg ggttagatag gacaggctgg aatagccggt gccgaagtcg tcgacggaga
    34921 tgcgcacgcc cgcgtcgcgc aggcgataga gcagccgcac ggcctggtcc ggctcctcca
    34981 tcaggcagct ctcggtgatc tcgagctcga gcagcgccgg tgtcacgccg gaagcggcaa
    35041 gcacgccgga aaccacctcg tcgaggtcgc gccggcggaa ctgcttggcc gacaggttga
    35101 ccgctacggg caccggtgcg aagcccgcct gctgccatcg cgcgatctgc gcacaggctt
    35161 ccctgagcac ccactcgccc accggcacga tcaggcccga ttcctcgagc gcgggaacga
    35221 actgcccggg cggcaccagg ccgcgcgtcg ggtggtccca gcgcagcagc gcctcgaatc
    35281 ccgtcagcgc cccgccgtcc agcgccactt tcggctggta gtgcagcacg aactcgccgc
    35341 gttcgatggc gtggcgcagg tcgttgttga gctgcatctt ctgcgccgag cgctggttca
    35401 tctcggcggt gaagaagcaa tacgcgttgc gcgtcgattc cttgacgcgg tacatcgcca
    35461 tgtcggcatt gcgcagcagg tcttcggcgt catagccgtc gccgggatac acggcgatgc
    35521 cgatgctcgc ggtggcgaag gcttcgctgc cggcgagctg gtagggctcg ccgagcgcct
    35581 cgagcgcctt ctgcgcgacc aggcccgcgt cgtccgcgtg cgcgagatcg gccagcacca
    35641 cggcgaactc gtcgccgctg atgcgcccca cggtgtcgcc cgggcgcacg cagcccgcga
    35701 ggcgacgcgc cgcttccgcg atcagcaggt cgcccgcgta atggcccagc gaatcgttga
    35761 ccagcttgaa ccggtcgaga tcgacgaaca gcacgcccac cttgcggtcg tgacgccgcg
    35821 cctgggtgag cgactgcgcc agccggtcgt gcagcagcag ccggttcggc agcccggtca
    35881 gcgtgtcgaa ctgcgcgagc tgcgccaggc gctgctcggt gagcttgcgc tcggtgatgt
    35941 cggatacgaa gccttcgagc gcgaccacct cgcccttggc gtcctgcacg cccacgccgc
    36001 gctcccagat ccatttctcg gcgccgtcgg cagtacggat gcggtactcc atggtgtagg
    36061 gcgcgcgctg gcgcagcgcc tcctgcgtgg agtgccagca gcgctcgcga tcttcctcgt
    36121 gcacgaggct gccgtaggca acgcggccgc tggtaaagtc ttcgggcgtg tacccggtca
    36181 gcagccgcgt gccctcgctc acgaactcca tggtccaggc ttcgtcgttg cggcagcggt
    36241 aggccgcgcc cggcaggctg gcgatgagcg tcgacaggcg gcgctcgctt tcgcgcagcg
    36301 cgagcatcgc ctcgtggctt tcggtcacgt cgcgcgcgat gccgcggtag ccccggaaca
    36361 cgccgtttgc gtcgtacacg ggctcgcccg atacgctgac gtaggccacg cgctggcccg
    36421 ctacggagcg cttgatcatg aagtcgtgga acgagccgcg cgcggcgagc accgcccggt
    36481 gctgcgtcca gtcgccttca aggggcgtgg tgccgggcag ctcccagcgc tgcagcccga
    36541 cgtgctgctc cgccgtgatg ccgccgcgct ccgcggcgtc gccgccggta tgcacgaagc
    36601 gcaattgcgc atcctgctcc cagtaccagt ccgaagacag ctgcgtcagc ctgcgaaagc
    36661 gctcctcgct ttcgcgcagc gactcctggg ccggcccgtc ggcgagcgcc gccccgagca
    36721 cgacgcccgc gcgcgccagc gccaccggca gctcctcggc gaaggcttcc ttcgacacca
    36781 cggcgtcgac gcccgcgcgc tcggcctcgg ccaggagctc ggcggtgggg aacagcgcca
    36841 gcgcaatgac gccaagcgcc gggttactgc gcttgagccg ccgcgccagg tccagcccgg
    36901 cgatgcccag gtcgacgagc accacgtcgc cggtggcgtg cgcgtagccc ggcaggcgct
    36961 cgacgaagcg tcgggccgcc cctgcaaagc cggggttgtc gtcgaggacg atgatgcggg
    37021 cgggcggtgc gctcggagcc atggcagccc gcactctagg tgccgggctc cggacccgta
    37081 ataaggccct tccctacgcg ccgtcgggga aaccccggta aggcccaagc ctttgttacg
    37141 tcttacgttt ccggcggaac gatgcccgcc cgcatggcgt agcgcaccag cccggcgacg
    37201 tcgcggatgc tgagccgctc catgatctgg gcgcggtgtg tttcgaccgt cttcaccgag
    37261 aggttcagtt cgaaggcgat ttccttggtg ctgcagccct cggcgatgcg cttgaggact
    37321 tcgcgctggc gcggcgtcag cggatcgggc ccggcgccca cgccgacgcg ctgcacgtag
    37381 ccgtcgacca cctgccggga aatggccggg ctgaggtagg tctcgccccg catcacgctt
    37441 ttcagcgcca gctccagttc cgaggtcgcg gaatccttga tcaggtaggc cgccgcgccc
    37501 gcgcgcagcg cgagcagcac gtactcctcg ctggtgtgca tcgaaaggat gatgaccttc
    37561 gcgaccggct ggcgctcgcg caggcgcgcc gcggcttcca gcccgttcat gcccttcatg
    37621 gcaatgtcca tcagcaccac gtcgggccgc tcgcgctcgg ccgccgcgag cgcctcggcg
    37681 ccgtcgctcg cctccgccac cacctcgacg tctgcgatgt cccggagcag cgagcgcatc
    37741 ccggcgcgca ccagggaatg gtcgtcaacc agcagcacgc gcagcgcggc cattttcaag
    37801 gcgctcccac cggaaacgac gccacgatca ccgtgccctg cccgggggca gaatggatct
    37861 gcagggtgcc gccggcaagc gccacgcgct cttccatgct gaccacgccc aggctctcgc
    37921 ccgccgcggc ccgtcgctgc gcccaggtca cgtcgaagcc ggcgccgtcg tcgcgcaccg
    37981 agagcgcgag ccgctcgccg gccaggaaca gccgcagcca tacgtttgcg gcccttgcgt
    38041 ggcgcacgat gttattgatc gcttcctgcg ccacgcgaaa gcatgccgtt tccacgtcgg
    38101 gagacagctt cagcggcacc tcctggatct caaagtgcgg cgcgaggccg ccgagcctgg
    38161 cctgtcggtc gaggtgcgag cgcagcgcag cggcgaggcc gagatcgtca agctgcagcg
    38221 gccgcaggtt gaccgagagc tggcgcacgc gctcgatcgt gtggcgcgcg gtctccagcg
    38281 cctcggcaat gcgcgtcgcg agcgccgccc cgtcgcgccc gcgtgccagc gcctccaggt
    38341 tgagcttgag cgccgtgagg ccctggccga tgtcgtcgtg cagctcgcgc gccaggtgtc
    38401 ggcgctcggc ttcctgcacc tcgagcagcc ggcgagacag ccactgcagg cgctcgcgcg
    38461 cggcgcccag cgcgtccatg gcgcgcttga gctccgtgac gtcccggccg acggactgga
    38521 tctcgcgcag cgcgccgtcg gcatcgaaca gtccgcgggt cgcccagtgc atccagcgct
    38581 cggcgccccc ggcgtcgcgc accagcgcgt cgcgctcgta tcccggggtc gagggcccga
    38641 tggcggcgag gaagcgctgc gcattgccac gttcctccgg ccgcatgtgc tcgagcaggc
    38701 tcttgcctag cacctgcgcc tgcgtgcgcc cgaaatagcg gcaatacgcg ccattcacga
    38761 atgttaccgt cagatccggc agcatgcgga tgatgagctc cgactgctcc tcgacaatgg
    38821 cgcggtagcg ccgctcggac agctcgagcg cccgctgcgt ggccaggtgc tcgcggcgct
    38881 ggcggttctc ctgcagcgcg cgcgccaccg ccggcccaag gcggttcagg ttgcccttga
    38941 gcacgaagtc ggtcgccccc gcgcgcagcg cctcgacggc gcgctcctcg cccagggcgc
    39001 cggagaccag gatgaacggc tgcaagccgt cgatcgcgcg cacccgcgcc agcgcatcaa
    39061 gcccggtgta gtcgggcagg ctgtagtcgg caagcacgac gtcgtagcgg tctgccgcgt
    39121 acagctgctc gaagtccgcg tgcgcctgcg cgatatccgc ggcgcagtcg aggcccgcgg
    39181 cggcgagcgc cgccagcgcc agctcggcgt ccgccggcga atcctcgagc agcagcaccc
    39241 gcagccgcgc catgcgctca gccgcacgcc ctcgggggcg gctcgttgac ccgcatccag
    39301 agcggtccca gctcgcgcac gacgctgcgc gcttcttcca gatcgcgctg cgcctgctga
    39361 tacagcaggt aggcggtcat cgcgacgaac ggcagcaccg gcacgcccac cagcagcgcg
    39421 agttcgccat ggatgctacg cggccgcagc cggaacgcca ttgcgccgaa cgccctcctg
    39481 ctccggagcg cgcagcgcgc tcccccgcgt cgattgtcgc ccgattggcg ggcatcgggt
    39541 cgcgcccacg cccggcaaat aggcattcgc atcaactatt taacattgcc gccaacggcg
    39601 tacaagaaaa caaggcccgt ctgtaggtcc gcgcgggcct cgaagaattc cgtggtggag
    39661 tggaagggga tcgaaccctc gaccttcgca ttgcgaacgc gacgctctcc cagctgagct
    39721 accaccccgt gggaagtcgc ggattttaca gcagatccgg cccgcgggaa cgattggagc
    39781 ggcgcattgc ggcggtcccg gcagggccgc tatgatcggc gcgttccctc ggggtatctc
    39841 ccattcagat caatccgcct ttcggctact cggaggtcgt tccactcctc aaaacccacc
    39901 gcgtgcgcct gcagcgcgcc ggcgaggcgc ccgcctttac gcgcgcgctg aacgcgatcc
    39961 cgatcagcta cacggagttc gcgcccgcgg cgcgcgacta tccgcttgtc ttcacttccg
    40021 cggacggcgg caagacctac gcgccggtcg cggtgcttgg catgcgccag ggcgagaacc
    40081 tgttcagcgc gcaggacgct tgggcgcagg acgtgtacat cccggcctac gtgcgccgtt
    40141 atccgttctg catggccagg gtccgcgtgc aggacgtcga gcgtcaggac cgcctcatct
    40201 gcgtcgagaa ggcttgcctt gaggacgcgg gcggcgaggc cttgttcgac gacaagggcg
    40261 agccgctttc cagatggcgg gatctgcaac gtctgctcgt cgagtacgag actgatctgg
    40321 agcgcggccg cgagctatgc acgatcctcg cggactacgc gctgctgcag ccgttcaaca
    40381 tgcaggcgaa gctcgaccag ggcgctcaga tgcaactcac gggaatgcac cgcgtcgacg
    40441 agacgaagat cgagcatctc aaccccagcc agttcaagaa cctggcgagg aagggaattc
    40501 tgggacgcat ctacgcccat ctgctttcgc tggagaactt cgcgcgcctg ctgcggcgcc
    40561 gggccgcgcg cgcgtccggc tgagcgctca gatactcttt gtgcgcaccg cgatcgccgg
    40621 aaggacgacc ctgacggaat tgagcgcggc gatttccaac tgcacttccc agtagtaaaa
    40681 gtgcaggtag ctcagcgcga gcgccacgat caacgccacc tcgcgcacca tgcgccggcg
    40741 ctcgtgctcg cggtggcctt ggggctggct gcccgccacc tccttgagga actccaggac
    40801 ttcggcgtgt gtctgcagct tgcgcccggc cagctcctcg acatagcggt gcagcgacgc
    40861 cggcgagtta taagtgtcgt cgccgttcgc cttggcttcg ctccgggccg gtgactgcgc
    40921 gcgccgtccg gcctccttca gccactgcgc cagtacctcg ccttcactat cgtgaccacc
    40981 gctcaagccg cctccctggg ctagtgccgc agaactgtga cacaggcgcc cggctcgcgt
    41041 ttatctgaaa gtccgctaaa cgccgccgtc ctgcttcatg acgcggcgga ttacatccgc
    41101 gacaatattc ttggtgtacg gcttggttat gtagccgtcc gcgccgagca gcaggccctt
    41161 gccgatatcc gcggcgtcgt tctccgcggt gagcatgacg atcggcaagg agcccagcac
    41221 cgcatggcgg cgcatcttgg tgagcaggtc aaagccgttg ccgtcgggca ggatgacatc
    41281 gagcagcagc aggtcaggtg cgccttcgtc gagcatgctg tgcaggaact cgttcacgga
    41341 cttcgccacg cgcaccttgt agccggccat gctgaccctc aggtcggcaa gcgcgagctg
    41401 gtccggatcg tcctcgacaa tcagcaccac ggtgtccgcg ggcgccttgc gtgccgccgg
    41461 gcggcggctg aggcgatcgg cggagatgta ggcgccggtg cgcgccagcg cggcgctggc
    41521 ggagtcgccg tcccggcgca tccgcgcctg gtcgaggcct agcgtgaggt cggactcgcc
    41581 cgccgagaac gccgatgcgt cgtcctcgcc ctcgagcgat gactcgatca ggccgaggtc
    41641 cttcatttcc gagagccatt cgttcaacac gttgcgcgga tagcggcgca gcagctcgcc
    41701 caccacgccg tcctggccgt ggaaatccat cagccacagg atgcgccggt agtcctccgg
    41761 cacggccatg tcctggctct cccaggcagc ccggccggcg gcggtcaatc gataggtgcg
    41821 cgctttctgt gccatcgcgt atatgcgtac tgtatcgcga gacggcgcca tagtgctccg
    41881 acgcgtaccg cgcataccgc gcataccgcg caaagggtta gcatgtgtcc cgatggattc
    41941 cgcccgcatg ctggccgtcg ccggaatgag cgaccccgtg agttccatga cgcatctggc
    42001 gggcgcgctg gcgtgtgcga cccgggccgg cgccctgctg cggagcgggc gcggcagcgg
    42061 cgcccgcctg ctatctcttg gcttgttcgc attctgctgc gtgctgttgc tcgctctgag
    42121 cggcgcctac cacctgctgg cgccggacac cgcggcaaag gcgctcctta agcggctcga
    42181 ccacgccgcc atctttatgc tgatcgcggc gagctttacc ccggtccacg cgattctttt
    42241 ccgcggcgct tggcgctggg gcatgctcgc cggcatctgg agcgccgcgg gcgcgggcct
    42301 gatcgtcaag acagcctatt tcgacgtcat gcccgaatgg gtggggctgt cgatgtacct
    42361 tgggctgggg tggctgggcg tcatctcggc agtggccctc gcgcggcggt acggattgcg
    42421 cttcatccag ccggtgctgt ggggggcgct cgcctacacg gccggcgcgc tcgccgagtt
    42481 ccatcgctgg ccgctgctcg tgcccggatt cgtcgggccg cacgaggtgc tgcacctggc
    42541 cgtgctggcc ggaatcgcct gccactgggt gttcatcgcg cgcattgccg cgagcgggaa
    42601 gagtgggccg ggttggattc gaaccaacga ccaagggatt attagagaga gtcgacccgt
    42661 tctcgacagc aaaccacagc actaagcagc agcactacag aggggaatcg aacagcgcgt
    42721 tagctgccga gtgctgccct ttgctggcgt ttcttccctg cgcgtgtgcc aagagtgtgc
    42781 caaggaatag atcccagacg caccaagaag cgccaagcac tccgctgccg ccgtgtgcgg
    42841 cctgagcgcg cccaggcctc gcgcagcgcg tcgtcgcgga cgcggtccag cacccgcctc
    42901 agcgatttca cggcgaacgg ctcgaggtgc gccacgtaca ggccgaaggc cgtgtggtta
    42961 taggaaagcg ccgggctgcc tgcgagatac aaaggatcga agtcggcggc gctcgggacg
    43021 tcgaactgca gggtgcggac cgtgatcggg tgcatgacac cgccctgttg gcgcgcgcag
    43081 aatcgcgagt atggcccacc cgccgcgctt gacggcaagg tgcggcgcga gaacccggct
    43141 tgacctagat cagcccgccc ggtcccgcgc ggcgcatagt gggttgcctt ccactagatc
    43201 tgcagcgcat gcgcaccctc tggctggtct gtgcactggc gatcgccctc gtcccgggtt
    43261 gcgccatcaa tcgcgagggc gcatccgtgt cgcccgacac cgatctctcc cagatccgga
    43321 agtaccacgt cgtcaggttc gcgccagacg cgcgcggcat cgaggcggtg attgccggcg
    43381 cgctgcgcaa gcgcggcctc gaggcgagct ccggcggcga ggacgccgcg cccggggacg
    43441 tccaggctct cgtcacctac caggacaggt ggcgctggga catcaccatg tacatgctcg
    43501 agctgaagat ctacatccgc gagccgcaga ccgggcggct gctggcgatg ggctcctcgc
    43561 accacacttc cctcagccgg cgtacgcccg aggaaatggc ttcggaggtg ctctcgaaca
    43621 tcttcaagga ggcaaggaaa tgacggcgcc cggagagtgg acgggccggc agtgggcccg
    43681 ggtagcgatg tccctcgcct tcctgctgct cgcggcgggc tgcgcgccgg ggatgcagaa
    43741 cgtgcgcgtg gaactgccca cctaccaggc tccgcgcgcc gcgcgtgccg cggagggacg
    43801 tccgacggtg cgggtcgagg ccgtcaagga ctcgcgcagc gattcggtgg gcagccatat
    43861 cggcgagcgc acgaccatcg gcaacatctc catgggcagc atcgagctgg agccgccgcc
    43921 gacggacgtg atcgcggcgc tcctgcgggc ggagttcgcg gggatgagcc acggtctcgt
    43981 gaaagcggac gagcggttca cggtcgccgc tcagctgcgc aagttccagg tgctcacgcc
    44041 ggcgacggcg ctctactggg acctcaacgg ggcgatcgag ctcgacctga gcgtgcgctc
    44101 acgcgaaggc cgcacccacg aggcccgcta cgcggcgacg tgcacggacc gcacctatct
    44161 cttcccgagc gaggacctca ttcgcaaggt cgtggcggcg tgcatcgggc gcgtgggcga
    44221 gtcgctgcgc ggcgatgcag cgctcgcgaa gttcatgggc ggaccgtagc aaaccgtctg
    44281 ggcggatagg ttcgcaggac gggcgcgcgc gacccgccgg cctgtagcat ccgcgcgtga
    44341 ccgcttcccc gtccgccgcg cgcgcctcgg ccctcgctcc cttcggcgtg cgcagtttcc
    44401 gcttccagtg gcccgccgac ctcgccacct cgtgggcctt atttaccata acgccggttg
    44461 ccccccggtg atccgcgcgc cttgcgcgcg cgccccacta gttctagcta gagagcgtta
    44521 ctcaccggct cgttgctgcg cccgccactc ccacggtggc ataggactag cgtcctgcca
    44581 tgcccgcggc gcgcgttctg cgcctctgct tgcagcgcaa ataggtaggc aacaagccta
    44641 gctctaccgc ttctccctgc ccccaaagtc tgctggcacc catcgcgcaa gccatatcac
    44701 aaggtactcg acgagccatc cagcaagcac cgaccaaaaa acggtaccgg ctaacccatg
    44761 ctgttttatg gtcttttata gtgggccggg ttggattcga accaacgacc aagggattat
    44821 gagtcccctg ctctgaccga ctgagctacc ggcccccgtg acgaacacgg gcgtcttgcc
    44881 gcccccgtat cccctgacgc caagctagaa gggccgcttg cgcggccctt cctttcttac
    44941 ccttcctgat cgaggaagct cttcagcctc tcggagcgcg agggatgccg cagcttcctc
    45001 agcgccttgg cctcgatctg gcgtatgcgc tcgcgcgtca cgtcgaactg cttgccgacc
    45061 tcttccagcg tatggtcggt gttcatctcg atgccaaagc gcatgcgcag caccttcgcc
    45121 tcgcgcggcg tcagcgtgtc caggacttcc ttggtgacct cgcgcaggct ggcgtacatc
    45181 gccgcgtcgt tcggcgccag cgtcgactgg tcctcgatga agtcgccgag gtgcgagtcg
    45241 tcgtcgtcgc cgatcggcgt ctccatcgaa atcggctcct tggagatctt gaggatcttg
    45301 cggatcttct cctcgggcat ttccatcttc tgcgcgagcg tcgccgggtc gggctcctgg
    45361 ccggtctcct gcaggatctg gcgcgagatg cggttcatct tgttgatggt ctcgatcatg
    45421 tgcaccggga tgcggatggt gcgcgcctgg tcggcgatcg agcgcgtgat ggcctggcgg
    45481 atccaccacg tggcgtaggt ggagaacttg tagccgcggc ggtactcgaa cttgtcgacc
    45541 gccttcatca ggccgatgtt gccctcctgg atcaggtcca ggaactgcag cccgcggttg
    45601 gtgtacttct tggcgatcga gatcaccagg cgcaggttcg cctcggtcat ctcgcgcttg
    45661 gcgcggcgcg ccttcgcttc gcccgtcgac atctgcttgt tgatctcctt caggtcgcgg
    45721 atcgagatgc cgatcttctg ctgcacgccc tggatcttgc ccagcagctc gagaatcgcg
    45781 ggctcgacgc gcgccagctg ctcgctccag gggtggttgg cgttcacctc gcccgagagc
    45841 cagcgcttgg agccttcggt gccgacgaat accttgatga agtgctggcg cggcatcagc
    45901 gccttgttca ccgagatgtc gtggatctgg cgctcgtggc tgcggatgtc gtcgaccagg
    45961 ctgcgcacgc tgtcgcacag gcgctcgacg gtgcgcgcag tgaagcggat ggtcatcagc
    46021 tccgccgaga tctggtcgcg cacgcgcagg tagtccttgt ccttggaccc cttgttctcg
    46081 agcacgcgct gcatgcgctc gaacagccgc cggatgcgca cgaacttctc cagcgcctcg
    46141 ttgcgcagcg cgagcaggct cgccgtctgc gcggcgccct catcctcctc gctctcgctt
    46201 tcctcctcct cgatctgctc ctcttcctcc tcctcgctcg cggcttcggc cttcttcggg
    46261 tcgaaatcgt ccttggcgtt cgggtcgatg aggccgtcca ccacctcgtc gatgcgcatc
    46321 tcttccttct ccaccttctc ggcgaggccg aggatctcgc gcaccgtcgt cgggcaggcg
    46381 ctgatcgcct gcaccatgtg gcgcaggccc tcttcgatgc gcttcgcgat ctcgatctcg
    46441 ccctcgcgcg tcagcagctc caccgagccc atctcgcgca tgtacatgcg caccgggtcg
    46501 gtggtacggc cgaactcgga gtccaccgtg gacagtgccg cctccgcctg ctcctccacg
    46561 tcctcgtcgg tcggcgcgac ctgcgccgac tcggcggtga tcagcagctg ctcctggtcg
    46621 ggcgcctggt cgtagacctg gatgcccatg tcgccgaagg tcgagatgat ggcctcgatt
    46681 tgctcggcgt ccaccaggtc gtcgggcaag tggtcgttga tctcggcgta ggtgaggaag
    46741 ccgcggtcct tgccgagctt gatcagcgtc ttcagacgcg tgcggcgcgt ctcggcgtct
    46801 tccttgggca gcacggccat cgccgcgagc cactcttcct ttttcgactt gcgccccggg
    46861 cggccgcgcg ccgccttgga cttcaggcct tcggcgagct tcgcctcgag ctgctcgagc
    46921 gccgccttgg cgctcttgat ggtgggcttt ttcgccttgt cgtcgatctt cgctgccgcc
    46981 ggcgccctgg agggcgccgc cttgtgcacc ttcttgtgga ccttctttgg cgccaggcgt
    47041 gcgggcttgc ctttgagcgg tttcttcgtg tgcgcgcgcg ctttcgacgt gggccgcgct
    47101 ttcttcttca agaccttgat agccttggcc tttttcccag ccttgcgcgg ttgcttcgac
    47161 ttcgccttgc gctttgcctt gaccattctc gcctcgtcgt cgcccaagtg ccgctgagcc
    47221 ggaaaaaagg ggcagattct accccaatcc gcctctcagg acctctgggc tttcagttgg
    47281 tgcagctctt tgacgcgaag atgcagctcc gcgttcaacg cggggttggc ttccacctgg
    47341 cccttgagcg cctcgatttc gaggtgcttg cggcggatgc gcagcgcgat ctgcagctgg
    47401 cggaactcct gcgccgccga ttccggcgtg accccctgct cgatggccga ctgctgcgcc
    47461 tggaacacgg ccttttcgaa ctgcgtcccc tggtagcgct cgaccagcac ggcgccgctt
    47521 gcaccttcgg cgcccgcgag gtccgccgcg agcgacctga gcgccgccga ctccggcagg
    47581 tccgggtcga gcagctcctc gtccagttcc tcggcgagcg cgggatgggt caccacgcag
    47641 cgcaagaggt gctgctcctc gctcttcagg atgggcgcct gcgccctggc gaccgacggc
    47701 cggcgcggcc gggcctcgcc ttccggcgcg aagaagttct ccgcgtactc gggtgcgacc
    47761 tgcgccagct cggcgacggc gcggatcagc tgctggcgca gagcgggcgc ggccaggcgc
    47821 tggagcaggg gcttggccgt gacctggaag cgcgcgcggc cttccggtgc agcgagatcc
    47881 gactgcgcgc gcagctcgcc gagcaggaag cccgagagcg tctgcgcgcg tgacatcagc
    47941 cgctcgaacg cctgctggcc gtgcgcgcgc acgaagctgt cggggtcctc gccctccggc
    48001 aggaacagga accggatcgc cgtggtgtcg gtggcgagcg gcaggctcac ctcgagcgcg
    48061 cgccacgcgg ccttgcggcc cgcgctgtcg ccatcgaagc agaacaccag ctcgctgccg
    48121 ctctcgcgca gctcgcgcag cagccgcgtg acgtgcaccg ggctggtcgc ggtgccgagc
    48181 gtggccaccg cgtaaccgac gccgtgctgc gcgagcccga tgacgtccat gtagccctcg
    48241 acgaccaggg cgcgcttggt ttcgcggatc gcttcgcgcg cgtgcccgag cccgtagatc
    48301 tcctgcccct tctggaagag cggcgtctcc ggcgagttca ggtacttcgg ctcgccctcg
    48361 cccagcacgc gcccgccgaa tcccaccacc gcgtcgcgtc ggttgaagat cgggaacatg
    48421 acgcggtcgc gaaagcggtc gtagcgcttg ccctcgccct cgatcaccag gccgcactcc
    48481 accagcgcgg cattctcgta atcggggaag gctcgcttga ggccctgcca gtcatccggc
    48541 gcatagccca ggccgaagcg cgccgccgtt tcgcccgaca ggccgcggcg cttcaggtag
    48601 tcgatggcgc gcggcgaacg cttcagctcc gcgcggtaga actccatcgc cttttgcatc
    48661 agcgcggcga ggtcggtctc gcgctctttg cgtttcgctt cctccggcgt gcgcggctgc
    48721 cattcgggca cctgcatgcc aaccatggac gcgagctcct tgaccgcgtc gacatagccc
    48781 atgccgccgt aatccatgag aaaaccgatc gcgttgccgt gcttctggca gccgaagcag
    48841 tggtagaact gcttggccgg gctcacagta aaggagggcg tcttctcgtt gtggaaggga
    48901 cacaggcccg agaggttggc gccggccttc ttcagctgca cgtagcgccc gaccacgtcg
    48961 acgatgtcga cgcggttgag caggtcttgc ttgaacgagt cggggatcac gggaaaatgg
    49021 ggacagacct cattttcttc ttgctgaaaa tgaggtctgt ccccattttc ttattttcac
    49081 cccccgagct tcgttttcac aagcgccgac accttgccca tgtcggcgcg cccggcgagg
    49141 cggggcttga gcagggccat caccttgccc atgtccttga ggcccgccgc gccggcttcg
    49201 ctcgcggccg cggcgaccgc ctgcgcgatc tcggcctcgc ccatctgctg cggcaggtag
    49261 gcgccgagca cgccgatctc gaacttctcc tgctcggcga ggtcctggcg cgcggccttc
    49321 tcgtactggg tgatcgactc gcgccgctgc ttcagcatct tttcgatgat gccgagcacg
    49381 tcggcgtcgg tgagcgccac gcgctcgtcc acttcctttt gcttcagcgc tgcgagcagg
    49441 aggcggatcg ccgacaggcg cgccgcgtcc ttggcgcgca tcgccgcctt catgtcttcc
    49501 tgaatgcgtt ccttgagggt catggggggc tccagaaatg caaacgcccc ggcgggctgc
    49561 accggggcgc ggtcgtcaag gtcgggctac gggcctagta cagcttcgcc ggcagcatct
    49621 ggctgcgcag gcgcttgtgg tggcgcttta ccgcggcggc aagctttctc ttgcgctccg
    49681 aggtcggctt ttcgtagaac tcgcgcgcgc gcagctcggt cagcaggccc gtcttctcga
    49741 cggtgcgctt gaagcggcgc atcgccacct cgaagggctc gttttccttg acgcgtacgg
    49801 tcggcatgca atgtcccgta aattcgaaag gccgaaattg tatcagaatc cccccgtgct
    49861 gatcctcggc atcgagacct cctgtgacga gaccggcgtg gcgctgtacg acgccgcggc
    49921 cgggcggctg ctcgcgcatg tggtgcactc ccaggtccag atgcatgaag cctacggcgg
    49981 cgtggtcccc gagctcgcct ctcgcgacca tgtccggcgc ctcgtgcccc tcgcccggga
    50041 cgtgctcgcg cgttccggcc gcgccgttgc gcagctcggg gcggtcgcct atacgcaggg
    50101 ccccggcctt gccggcgctc tgctcgccgg ggccgccttc gcccacgcgc tcgccgcctc
    50161 gctcggcgtg ccggccatcg gcatccacca tctcgagggg cacctgctct cgccgttgct
    50221 gtccgcacgc aggacggcct tcccgttcgt tgcgctcctc gtctcgggcg ggcacacgca
    50281 gctgatgcgc gtcgcggccg tgggccgcta cacgctgctc ggcgaaacgc aggacgacgc
    50341 agcgggcgag gccttcgaca agaccgcgac gctgctcggc ctgggctatc ccggcggccc
    50401 ggcgctcgcc cgcctcgccg agcgcggcca tgccgggcgc taccgcctgc cgcggccgat
    50461 gatcgcgagc ggcgacctgg agttcagctt cagcggcctg aagaccgccg tggcgacgct
    50521 ggtaaagggc ggcggaccgg acgcgcagtt ccacgcggac gtcgcgcgtg cgttcgagga
    50581 tgccgtggtc gaggtgctgg tggcgaaatg cgccctcgcc atggagcgca cgggattgac
    50641 gcagctggtg gtcgccggcg gcgtcggcgc caaccgccgg ctgcgcgccg cactcgatgc
    50701 ccgggggaag tcgcaggggt tcgacgtggt ctatccggaa ccc
//
LOCUS       DBNHKE010000128         6549 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_497310, whole genome shotgun sequence.
ACCESSION   DBNHKE010000128 DBNHKE010000000
VERSION     DBNHKE010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_497310"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="AC16O9_08365"
     CDS             complement(<1..1585)
                     /locus_tag="AC16O9_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516666.1"
                     /translation="MKSLVDPYAAGIRVLQYALLAIACGALYGGFLSSPLVLDDLILR
                     EFGRPETLKTILGLELRWLPYASIEWTRKLTADSLHWLRLGNLALHVANTLLLFLFLD
                     RLFASVLPGEAGIRASGRDALPSTWLAFSGALLFALHPVAVYGVAYLIQRTILMATLF
                     SLATWVLFLEGLIRDQRRWLWASALAYALAIFSKEHAVMAPAVALALLLLLRGPDKRL
                     LAQVWPIFLVYAATALLIVLPMKLFGIIGNAYEPDGARLLATQQVDPRNPYPLALATQ
                     GYLYFKYLLLWIVPNPAWMSIDMVERFAPRLWAWPETAGLLAFVLYPLAALRLLWQRG
                     RPGLLGFALLAPWLMFATELSTVRIQEVFVLYRSYLWMPCLFAGLPFLLQKLSARRSL
                     FVLGAIVLALVPASWIQLTRFADPLLLWSDALDLARDKDNPIKLGRIYHNRGLAYLDK
                     PRYEDAIVDFGKGIRLLPMHHLLYNDRAVAYLMTRRYREALKDYNVAILLNSGYYRSY
                     WGRAMVFEALGEEAAARNDY"
     gene            1952..2134
                     /locus_tag="AC16O9_08370"
     CDS             1952..2134
                     /locus_tag="AC16O9_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516667.1"
                     /translation="MNTADVAAMARHLFEAHGPKAIAEAGQKAASFETAGDKAQANFW
                     RRVGDALQEMRGARES"
     gene            2216..2413
                     /locus_tag="AC16O9_08375"
     CDS             2216..2413
                     /locus_tag="AC16O9_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516668.1"
                     /translation="MRLPIAFAVALMLAGCGMETASTAATAASLKKQEMEQGQKTMQQ
                     AQQRIDGAMQQLQDRAAGADK"
     gene            complement(2423..2704)
                     /locus_tag="AC16O9_08380"
     CDS             complement(2423..2704)
                     /locus_tag="AC16O9_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516669.1"
                     /translation="MRIRALIAGIAALASIAGAAIAFLWQGIIYLQSSAWPKLSLATA
                     LRWLDARSWARLAHTWPDGHALLDAIPLSIALVGLAVLAFAVARWGANR"
     gene            2918..3877
                     /gene="corA"
                     /locus_tag="AC16O9_08385"
     CDS             2918..3877
                     /gene="corA"
                     /locus_tag="AC16O9_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR6516670.1"
                     /translation="MLYRPDSRLVATGGEELVAQWRERPDGILWADYFDNPPESEQRA
                     LVENFGLHPLAIQDAQRRRHQPKFEAFGDHVFILLKGLGPDRDEFEFETIQIAIFIGE
                     RFLVTRHSGHSPSIDRLREQLQQDPSLFASGPDALALRLSRISVDRYLKKLLSLEPRL
                     EDIEQEIVASPRDEILSDLMGYKTNLRKFRRVLLYHVQVFLEMMKDPPPQVRAERIHE
                     IKDVYENQERANSLATLYYEVATDLIEGYISLASHRLNNIMKILTIVTAIFVPLSFLA
                     GIYGMNFEYMPELKSHTGYFVLLGVMGAIATILLLVFRKKRWL"
     gene            complement(3956..4555)
                     /locus_tag="AC16O9_08390"
     CDS             complement(3956..4555)
                     /locus_tag="AC16O9_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516671.1"
                     /translation="MLAAGCISNPRDGAIAPPLDPKRKVSEQDCSRPLDDDGGNLLCR
                     EITEAERLARIAEAERQARARLEAAARAERERLERERAEQLAREEAARKAREAEEARL
                     AAERRRLEEEAARKAQEERERREKAEAEERARLKAEQEAREAAARECRRLEQERSEGR
                     SLLAVFVADYGKDEEFKEIAAQIAAFLAKPRKAKPCPPA"
     gene            5300..5896
                     /gene="thrH"
                     /locus_tag="AC16O9_08395"
     CDS             5300..5896
                     /gene="thrH"
                     /locus_tag="AC16O9_08395"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HIR6516672.1"
                     /translation="MPLIVCLDLEGVLVPEVWIAFAEKTGIAELRRTTRDEPDYDKLM
                     QGRLAILERHGFKLRDVQDVIATLKPLDGAREFLDELRARTQVIIVSDTFYQFAGPLV
                     RPLGWPTLFCNELLVEPSGRVAGYRLRVPDGKRRAVASLKALNFTVHAAGDSYNDTTM
                     LCEADRGVLFCCPDAVAQEFPQFQRTETYAQLKDALLA"
     gene            6035..6472
                     /locus_tag="AC16O9_08400"
     CDS             6035..6472
                     /locus_tag="AC16O9_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR6516673.1"
                     /translation="MTSGPVDGKDSGNKFFGGYQFSRNLGLELAYADLGKATYSGTFL
                     GIPVTGGTAKTTGLNFSAVGTMPLNEKFELFGKIGLFAWESKASDVTGGLPFSGKADG
                     SDLSFGFGATYNLTRNFGLRAEWEQFKAVDNISLLSIGAAYKY"
BASE COUNT         1194 a         2179 c         2111 g         1065 t
ORIGIN      
        1 catagtcgtt gcgggcggcg gcctcttcgc ctaatgcctc gaacaccatg gcgcgacccc
       61 aatacgatct gtagtaaccg gaattgagca ggatggcgac gttgtagtct ttcagcgcct
      121 cccgataccg tcgcgtcatc aggtaggcca ctgcccggtc gttgtagagc aaatggtgca
      181 tgggcaagag ccggatccct ttgccgaagt ccactatcgc gtcttcgtaa cgcggcttgt
      241 ccagatacgc gagtccccgg ttgtgataga tcctgcccag cttgatgggg ttgtccttgt
      301 cccgcgcgag atcaagggca tcgctccaca gcagcagcgg atcggcgaag cgcgtcagct
      361 ggatccaact cgcgggaacc agggccagga cgatcgcgcc aagtacgaag agcgaacgcc
      421 gggccgagag cttttgcagc aggaacggca agccggcgaa caggcagggc atccacagat
      481 agcttcgata gagcacgaat acttcctgga tgcgcaccgt cgagagctcc gtggcgaaca
      541 tcagccacgg cgcgagcagg gcgaacccga gcaggcccgg gcgcccgcgc tgccacagca
      601 ggcgcagggc cgccagcggg tacaggacga aagcgagcag cccggcggtt tccggccagg
      661 cccacaggcg gggggcgaac cgctccacca tgtcgatcga catccaggcc gggttcggca
      721 cgatccacag cagcaggtac ttgaaataca gatagccctg tgtcgccagc gccaggggat
      781 agggatttcg tggatcgacc tgctgcgtcg ccagcaacct cgccccgtcg ggctcataag
      841 cgttgccgat gatcccgaac aacttcatcg gcagcacgat gaggagcgcg gtcgccgcat
      901 agacgaggaa tatcggccac acctgcgcca gcaggcgctt gtccggcccg cgcagcagca
      961 agagcagggc gagcgctacg gccggcgcca tgacggcgtg ttccttggaa aagatcgcga
     1021 gcgcataggc cagcgcggac gcccacagcc agcggcgctg atcgcggatc aacccctcga
     1081 gaaacaggac ccaggtcgcc aggctgaaca gcgtcgccat caggatggtg cgctggatca
     1141 ggtaggccac gccatagacg gcgacgggat gcagggcgaa gagcagcgcg ccggagaatg
     1201 ccagccaggt cgagggcagc gcatcgcggc cggaagcgcg gatgccggct tctcccggaa
     1261 gcacgctcgc aaacagcctg tcgagaaaca ggaacagcag caaggtgttg gcgacgtgca
     1321 gtgccagatt gcccaggcgc agccaatgca gcgagtcggc ggtcagtttc cgcgtccact
     1381 cgatactggc atagggcagc cagcgcaatt cgagacccag aatcgtcttt agcgtctccg
     1441 gacggccgaa ctcgcgaagg ataagatcgt ccagcaccag cgggctggac aggaaaccgc
     1501 cgtagagggc gccacaggcg atggcgagca gcgcgtactg caacacccgg atgccggcgg
     1561 catacggatc gactagggac ttcatcttct agcaggcgcc gctgcagcgg gcgcagctcg
     1621 cgcggacgat gccgccactt gcactgctcg cccggcctgc gcaacgcccg gtcggcgcgc
     1681 ggccgcgctt cggtcccgac gccggcgcgg atcgcctgtc tcggcaacga aaagcaccat
     1741 cgatccgaga ccagccgcaa atcggcccat gttgcacccc cccgattgcc agtgtgccag
     1801 ccccgagcgt cttgcaaaca cttgagaaaa tcaatcagcc tcttacggcg acaggccgag
     1861 gccgagtatt tccgccatgg gctggcagtc tagcccaccg gttgaaccca atgcccgcgc
     1921 ggctgccaaa cactcaaagg aggcaaggac gatgaacact gccgacgttg ccgccatggc
     1981 gcggcacctt ttcgaggcgc acggccccaa agcgattgcc gaggcggggc agaaggccgc
     2041 ttctttcgag accgccggcg acaaggcgca ggcgaatttc tggcgacgcg tgggtgacgc
     2101 gctgcaggag atgcgcggcg cgcgggagag ctagttccgg ggtcgtgaac cagttcgctg
     2161 cgtgatgcgg cggatcgggg taaggtaccg gcgctacctc aatcggaggc cggtcatgag
     2221 actgccaatc gcgtttgccg ttgccctgat gcttgccggt tgcggcatgg aaaccgccag
     2281 caccgcggcg accgccgctt cgctcaagaa gcaggagatg gagcagggtc agaagacgat
     2341 gcagcaggcg cagcagagaa tcgacggcgc catgcagcag ctgcaggacc gcgccgccgg
     2401 cgccgacaag tagccgggcg ccctagcggt tcgcgcccca tctcgcgacc gcgaacgcga
     2461 gcaccgcgag ccccaccagc gcgatcgaca ggggaatcgc gtcgagcagc gcatgaccgt
     2521 cgggccaggt gtgcgccagg cgcgcccagg agcgcgcgtc aagccatctg agcgcggtag
     2581 cgaggctcag cttcggccag gccgagctct gcaggtagat gatgccctgc cacagaaacg
     2641 cgatcgccgc gcccgcaata ctggccagcg cggcaatgcc ggcgatcagc gcccggatcc
     2701 tcattgcgcc gcccactcta caccgggtct ggcgtccaca cgggggatat cgccgataag
     2761 actcgcttcg ctagcgtcgt cacgggcgcc cgcgtacgcc gcgaagggtg gcgccgctcg
     2821 gcatagttcg gctcgcggca tggggtacca tcgtaaagaa gatcatggcc gtccacgatc
     2881 atacgttcgg tcacgggggt gccgcatgat tcacgccatg ctgtatcgcc ccgatagtag
     2941 gctcgtcgcc acgggaggcg aggagctggt cgcgcaatgg cgcgagcggc ccgacgggat
     3001 cctgtgggct gattacttcg acaacccgcc cgagagcgag cagcgagcgc tcgtcgagaa
     3061 cttcgggctg cacccgcttg cgatacagga tgcgcagcgg cgccggcacc agccgaaatt
     3121 cgaggccttc ggcgatcatg tcttcatcct gctcaagggg ctcgggccgg accgggacga
     3181 attcgagttc gaaaccatcc agatcgcgat cttcatcggc gagcgcttcc tggtgacgcg
     3241 ccactcgggg cactcgccca gcattgatcg gctccgggag cagctgcagc aagacccgtc
     3301 gctattcgcc agcggccccg acgcgctcgc gctgagactt tcccggatct cggtggaccg
     3361 gtatctcaag aaactgctgt cgctcgagcc gcgcctcgag gacatcgagc aggagatcgt
     3421 ggcgagcccc cgcgacgaga tcctttcgga cctgatgggg tacaagacca acttgaggaa
     3481 gttccgccga gtgctccttt atcacgtgca ggtgttcctg gagatgatga aagacccgcc
     3541 cccgcaggtg cgcgccgagc ggatccacga gatcaaggac gtctacgaga accaggagcg
     3601 ggccaacagc ctcgcgacac tctattacga ggtggccacg gatctcatcg agggctacat
     3661 ctccctggcg tcgcaccgcc tcaacaacat catgaagatc ctcacgatcg tcaccgcgat
     3721 cttcgtgccg ctgagcttcc ttgccggtat ctacggcatg aacttcgaat acatgccgga
     3781 gctcaaatcg cacaccggct acttcgtgct gctcggcgtg atgggggcca tcgccaccat
     3841 cctgctgctc gtgttccgga agaagcgctg gctgtgaggg cgcggaacgg gcgggccgcg
     3901 gcctacgcgg gtccggaggt cactgtcacc cctgggagcg cggcccgtcg cgccctcacg
     3961 cgggcgggca aggctttgcc ttgcggggct tggcaaggaa cgcggcgatc tgcgcggcga
     4021 tttccttgaa ttcctcgtcc ttgccgtagt cggcgacaaa taccgcgagt agagaacggc
     4081 cctcgctccg ttcctgctcg agacgccggc attcgcgagc ggcggcctcg cgcgcttcct
     4141 gctcggcctt caggcgggca cgctcttccg cctccgcctt ctcccgacgc tcgcgctctt
     4201 cctgggcctt gcgcgcggcc tcttcctcca gccggcggcg ctcggcggcg agccgcgcct
     4261 cctcggcctc ccgcgctttc cgggcggcct cttcgcgggc aagctgctcg gcgcgctcgc
     4321 gctcgaggcg ctcgcgctcg gcgcgcgccg cggcttccag ccgagccctg gcctgccgtt
     4381 cggcttccgc gatcctggcg aggcgctcgg cctcggtgat ctcacggcac aacaggttgc
     4441 cgccgtcgtc gtcgaggggt ctgctgcagt cctgctcgct cactttgcgc ttcggatcca
     4501 gcggcggcgc gatggctcca tcccgcggat tgctgatgca gccggccgcg agcacgagga
     4561 ggaggacgac gacggttatg ttcttggtca tggctctcct ttcaggttcc ataggcgcgc
     4621 agcgccagaa ctgcgtggtt cgacctcgaa gcgtaggata cgcgcagcgc gctcggcgcg
     4681 ctgcgacaca gtccgcacat cggcagcggc gcctcgacaa cagccacggc aggcggttca
     4741 agcggcgggc cccaaattga aggacgtgca acggcactca aatcgctcgt cgtccgaatc
     4801 atcccgtcgg caccctctcc gttcccacac ttgccgccgc actcaagccg gtcggcggcc
     4861 atggcctcag ccggtctatt gttgcgcaac gaacttcgcg ggctcccaac ggccgcccgc
     4921 gtgatcctac gccactctcg atcataacgg cggttgcgcg gcggcgctga aggccgcgcg
     4981 catcacccac tccaacacac agcccccctc actgaccccc gccgcgcccg ggtgaggtcc
     5041 tcgagaggtt gcggggcgcg ggcagctcta tcgaacgcat ccgctcttgc tggactgcct
     5101 tcgccccgtg gtaccctgca cacggcttcg cgcccgaggg cgtgaagaat tccatgcttt
     5161 acccgattca agaagcaccc gcgccatagg gcgatcgaag gtctgtgggc cggaagcttg
     5221 aagcgatgag cgccaaggca gcccgtcgcc gctcgcgtcc ggccgagccg ctcgaggcgg
     5281 gacggaggag gaacaccgcg tgccgctgat cgtttgtctg gacctcgaag gcgtcctggt
     5341 tcccgaggtc tggatcgcgt tcgccgagaa gaccggcatc gccgagctcc gtcgtaccac
     5401 gcgcgatgag ccggactacg acaagctcat gcagggccgc ctggcgatcc tcgagcgcca
     5461 cgggttcaag ctgcgcgacg tccaggacgt catcgcaacg ctcaagcccc tggatggagc
     5521 gcgcgagttc ctcgacgagc tgcgcgcccg cacccaggtc atcattgtca gcgatacctt
     5581 ctatcagttc gccgggccgc tcgtgcgtcc gctcggctgg ccgacgctgt tctgcaacga
     5641 gctgctggtc gagccttccg ggcgcgtcgc cggctaccga ttgcgcgttc cggacggaaa
     5701 acgccgtgcc gtggcgagcc tgaaggcgct gaacttcacg gttcacgcag cgggcgacag
     5761 ctacaacgac accaccatgc tgtgcgaggc ggaccgcggc gtgctgtttt gctgccccga
     5821 tgcggtcgcg caagagttcc ctcagttcca gcgcacggaa acctacgccc aactcaagga
     5881 cgctctgctc gcgtaggccg caggcgagcc gacggttcgc tattttccgt aatgccgagt
     5941 tcgtgcgcct acggcctcct tagcgactcg gacgcgtggc acatgatttg atccaaatca
     6001 agatgcggat gatggcaacg ctatccctga cctgatcacg tcgggaccgg ttgacggcaa
     6061 ggactccggc aacaagttct tcggcggata tcagttcagc cggaacctcg ggctggaact
     6121 cgcgtacgcg gatctcggca aggccaccta cagtggcacg tttctcggta tccccgttac
     6181 cggcggcacg gccaaaacaa cgggattgaa tttttccgcc gtcggcacca tgccactgaa
     6241 cgagaagttc gagctctttg gcaagatcgg gctatttgct tgggaatcga aggcgagcga
     6301 cgtgaccggc gggctgccgt tttccggaaa ggcggatggt tccgacctgt ccttcgggtt
     6361 cggcgcaacc tacaacctga cgaggaattt tggcctgcgc gcggaatggg agcaattcaa
     6421 ggcggtggac aacatcagcc tgctctccat cggcgccgcg tacaagtact gattgcccgg
     6481 ccgcgctgac gcgttgtacg tcagcgcgtt gcccgacccg gttcgagcgg ctggtccggc
     6541 gctagcctg
//
LOCUS       DBNHKE010000129        35756 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_709014, whole genome shotgun sequence.
ACCESSION   DBNHKE010000129 DBNHKE010000000
VERSION     DBNHKE010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 35756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35756
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_709014"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="AC16O9_08405"
     CDS             <1..405
                     /locus_tag="AC16O9_08405"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR6516674.1"
                     /translation="DRLTRTPAVVHVVPGQVIEIPVARDGHYYVEGSVNGTPLKFMID
                     TGATYVSVSGKFAAAARLPEGVTGYFSTANGTVEGKIVKDQVVEAEGFKVSGLSVAVT
                     PLGSKQGLLGQNFLKRFEVSQSAGVLRLRQAQ"
     gene            425..1408
                     /locus_tag="AC16O9_08410"
     CDS             425..1408
                     /locus_tag="AC16O9_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6516675.1"
                     /translation="MKLRRFGRTGLQVSEVVFGAGYVGGIIVLADDDTRRRALRRALD
                     AGINWIDTAPLYGQGRSEEALGWLLAEVKEKPYLSTKVRLDLGKLADIPGEIERSVHE
                     SLKRLKRDSVDLLQLHNQIGAKTGSGVLGAVSIDDVLKKGGVADGLERMREQGLTRYI
                     GLTALGEAPSIIRAIDTGRFDSAQVYYNLLNPSAARAMPKGWSGHDFSGVCAACKRHD
                     VAMMNIRVFASGVLATDERHGREIALTENSALEVETKRAHAALAALGTGHGTRAQTAL
                     RFALANPDLACVVIGLAELSHLEEAIAAEAKGPLPADVIEKLEPVYARGFA"
     gene            1429..2193
                     /locus_tag="AC16O9_08415"
     CDS             1429..2193
                     /locus_tag="AC16O9_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516676.1"
                     /translation="MPKTVLITGAGSGFGRAAAIELAKRGHKVIATVESDAQAAELSK
                     AHPELTVAKLDITNARDVATLDKWDLDVFIANAAMGQTGPLSVIPLERLRKVFEVNVF
                     GTFACVQRVAQKMRKKRSGRILIVSSIAGVRAGVGSGPYAMTKHACQALGMALRAELT
                     AFNVDVALINPGPYATGFNDRMANDPGEWFDRKTAHPEDVKAMDSLVQRITVGQMDPK
                     EVVQRYVELVEADQTELVNFIPPDIVERMSRPKPAR"
     gene            complement(2203..2934)
                     /locus_tag="AC16O9_08420"
     CDS             complement(2203..2934)
                     /locus_tag="AC16O9_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR6516677.1"
                     /translation="MPDPGPGAQSSGAEAPKPMPFAVMRNSHEALRASVRLQEEKLEA
                     GDAKGLGDEWRTFQRGLAVHMAMEDKFMFALLDEVGAGAISAAGVPAEHVEDLRLARA
                     VDAALAANDLGALRSAWPAWRDDHLHHLTHEEDVMMPLTMKTAPTPEARARVFHDRVL
                     APSEKLPDFDWYIGWVVRMLSGQGTAAQPASIAVRVFAWGLQHACSPAQWRRLAPIVK
                     SNCAPAIWTEIAAKFGLEGEGKISP"
     gene            complement(2995..3768)
                     /locus_tag="AC16O9_08425"
     CDS             complement(2995..3768)
                     /locus_tag="AC16O9_08425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516678.1"
                     /translation="MKARFQDKVIVITGAAQGIGRAAAMRFAADSARVVAVDLPGSDL
                     AGCVAAIKKAGGQGMAVEADVTRKADVENYVARAESEFGGVDCFFNNAGILGVNRPLL
                     EYPEETFDRVFAVNVKAVWMGLKLVAPAIIRRGGGAIVNTASIAGLRATAGLFAYTAS
                     KHAVVGMTRTASIELVKQGVRVNAVCPGPIETPMGEQLDRGLDPQNPKAGHERSVARI
                     PMGRYGRPEEVAGLVAFLCSAEASFVNGGIYTVDGGAMS"
     gene            complement(3771..5219)
                     /locus_tag="AC16O9_08430"
     CDS             complement(3771..5219)
                     /locus_tag="AC16O9_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6516679.1"
                     /translation="MSRDLVVVGCGIAGMSAAVSALQAGLQVTLLERAPEEDFGGNTR
                     WTEAYLRMKNDAEVSDDFEEKFAENAGANLDPNVLDAVAGEYAHWPAYVKAHPLPDPE
                     LIAHFARSVPPTIAWLKSFGLRFAPQPIYLLTQSTSRIAAQGGGLALIERLMQQAKAL
                     GAEVHFRTTAVDLVRAADGRVAGIVAIDEAGKRKEIRARATLLASGGYEGNPEMMTHY
                     MGTRAAHIRPVARGGYYNKGEGIRMALAAGAAPAGDFGSYHAEPVDPRSRQPEAVVHI
                     YPYGILVNKLGQRFVNEGPGMVDAWYDPITRTVADQPDGICWVIFDAQVEEIPRWKTS
                     IRSDVPPYEAATLEALLEKLGLPAETLETIAAFNRACPPAAGEFEPFAPDGRAARGLV
                     PAKSHWCRPIVKPPFRAYPIISANCFTFGGLKVNTHAQVLDSDGKVIPGLYAAGETVG
                     LYHQVYTGSTSVLRGAVFGRTAALHVAAAKKE"
     gene            complement(5216..5674)
                     /locus_tag="AC16O9_08435"
     CDS             complement(5216..5674)
                     /locus_tag="AC16O9_08435"
                     /inference="COORDINATES: protein motif:HMM:NF023010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HIR6516680.1"
                     /translation="MPELKSAYIGELMMEITGSYMLGDAPVGRRRLDRLDKGHFKGPK
                     VQAEMLTGGMDILLGGADGAVRPDVRLILKLDDGEILLIQYRGVRHAPAEVMQRIGKG
                     ERVPPGEYYLRTSLVFETASKKYGWLNRIVGVGVGRREPSAVFYDVFEIL"
     gene            complement(5686..6504)
                     /locus_tag="AC16O9_08440"
     CDS             complement(5686..6504)
                     /locus_tag="AC16O9_08440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6516681.1"
                     /translation="MAGPLLTREVHDALRNAARAGKPAVECSLDLGRSTTTVEVDADG
                     WRWQGRRFAYLESCKARTIYYWVDDAFQPVQRFTASLVKLVPTHWGPPTFEIDGIKMH
                     PTEHISPYVDAERKAGLVRPRGKVILDTCGGLGYTAAACLKGGARQVLSYEKNEDVIW
                     LRGLNPWSPEAGGALALTHGDIAQHLPTLPDGSVDAILHDPPRFGIAGELYSQAFYDE
                     LARVLKRKGLLFHYTGSPNKLTSGRDVPREVAKRLRSAGFAAELRGDGVLATKT"
     gene            6680..7099
                     /locus_tag="AC16O9_08445"
     CDS             6680..7099
                     /locus_tag="AC16O9_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6516682.1"
                     /translation="MGNIVGGCLCGAIRYTSKAQPLLTAVCNCKHCQRQTGTAFSVLV
                     ALPKGTLEFSGNRPATYHDTGSSGLPVLRHFCANCGSPIFSDVAATPAMDWLKAGTLD
                     DASWLQPQVSIWCDSAQPWVQMSESIARFPQNPPPAA"
     gene            7317..7637
                     /locus_tag="AC16O9_08450"
     CDS             7317..7637
                     /locus_tag="AC16O9_08450"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6516683.1"
                     /translation="MPKYMLRVNYTADGAKGLIKDGGSTRRSIAQKAAESVGGRVESF
                     HFAFGDTDAFVIADLPDATSAAAIALTVSASGGASVQTTVLMTPEEVDAAVRKSPLYS
                     APGR"
     gene            7804..8367
                     /locus_tag="AC16O9_08455"
     CDS             7804..8367
                     /locus_tag="AC16O9_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR6516684.1"
                     /translation="MQAFITSALVVALAEMGDKTQLLSFLLAAKFRHRMPIILGILFA
                     TLANHFLAGYVGVWLASLVSPQALRWIVAVSFFAFGAWALKPDKLEDRKLGGAGVFVT
                     TLIAFFLVEMGDKTQLATVALAAHYDALVPVVVGTTLGMMIANVPAVWIGEALAHRVN
                     MTVMRWVAAALFALLGVIVLIAPTPAA"
     gene            complement(8387..10111)
                     /locus_tag="AC16O9_08460"
     CDS             complement(8387..10111)
                     /locus_tag="AC16O9_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6516685.1"
                     /translation="MARYHYCMNKTIDAGAATLTGAEAVAVMLNVHGVEVIFGLCGDT
                     SLPLYDALARLPHGMKHVLTRDERHAAYMADGYARVSGKVGVCEGPSGGGATYLLPGL
                     VEANESSVPVLGINTDVSVSSRGRFTLTELDQKALMRPLTKWNAVLDRAADIPRVFRA
                     AFNAMTTGRPGAAHIALPFDVQNAPVERGDVWADPSLGSYPSRRIAPDPFHVELAAKL
                     LRAAKRPVFICGGGPVISGAQEELLELAMRLSAPVATTISGQGSISEEHELAVGVVGS
                     NGGTPETRAIVDTADLVVFVGCRAGSVTTERWRHPVPGKAKILHIDVDAQTIGTNYVA
                     DVPLVADARLALAALNAALDDLRRPLEAEAVAKAKAAKLAKFEALASSGERPIKPERV
                     VQALSQVLDADAIVVADPGTPCPYLSAYYPVRKAGRTLFSNRAHGALGYSLAAAVGAH
                     FARPSVKTVAVMGDGSFGMCAGELETLVRYKLPITLVVIRNAVYGWIKAGQKTGYGQR
                     YFSVDFGVTDHAKVAQAFGVKSWSVEDPAQLGAALKEAVAFDGPTLVDVACQPLQEAK
                     APVSEWVA"
     gene            complement(10179..11966)
                     /locus_tag="AC16O9_08465"
     CDS             complement(10179..11966)
                     /locus_tag="AC16O9_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516686.1"
                     /translation="MQAASGATVVGVSFWRTMSGHVVAWFLLLLVSAAIYAPFLNNPP
                     VFDDLGFFTPDLLAQAAGTSPVLGWSLRWISYGSFGWTYALVGLDIPWFRLGNLLIHA
                     CVGIALYHFLGIVLRMAGGERPLRRANDAALIGALWFVAHPVAVYGVGYLVERSIAMA
                     AWFSLLSLIALAHGVIERRRWSLACSALFFLLAVFSKEHAVALPAVAALIVILAGPAA
                     RRAKGELAALFVAYGLIALFVTLRVKGLLGAPYEPAAMRYFEHLARGGTRVDVDNAHL
                     LSIQTQAGLFFDYLKIWLLPLPQWLAVDLRRPFATVFAGLGGVAGMAAFAAYPVVCWL
                     LVRRGGLAAAAALGLMFPWLLFCVEFTVVRIQEPFVLYRSYLWMTGCALVPAVAAYAP
                     GRVVRFALAAAAVLLLALGATGRLTSFSSEFALWNDAVAKQSDESLPGAWRAFANRGA
                     MHGSAQRWPEALADLNRALHLYPDYASALVNRGNVHAALGRREEAMADFTRGHALARK
                     NGPAEMAAYALARRGTLQWQSGSSLAALKDLRAAVQLDPRSANIRFQFGQYLLDSGYQ
                     REAAEQLGMACALGMNQACALQQSPQGRS"
     gene            12132..12989
                     /locus_tag="AC16O9_08470"
     CDS             12132..12989
                     /locus_tag="AC16O9_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6516687.1"
                     /translation="MRIADRQRPPPGQLYLDHVSHFVPDLDKAGALLEALGFAVTPLS
                     VQVTQQGPAGTSNRCVMLADGYLEFLMPTADTPNARRLKKSMRRYRGVHLVCFGTPAA
                     EQEHQRLAAHGFAPQPIVHLKRRVERGLRARFSVVRAAPGKMPEGRVQYVQHLAPAAI
                     WRPQHLRHANGVLGLAAAYIVAANPVRAAARWSRFMGGLPRREGRLVSLDTDRGKVLI
                     GTRAQLKALLGAAPRSPGIAGYALACRDPKAFARRCAQAGLKVNRHLAVALPRALGGA
                     WRLVPMEKR"
     gene            12986..14380
                     /locus_tag="AC16O9_08475"
     CDS             12986..14380
                     /locus_tag="AC16O9_08475"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6516688.1"
                     /translation="MKTDLCALSATELLAAYRAKTISPVDATRAALERIAKLNPVLNC
                     FNLVDEKGALAAARESEARWKKGAPRGLLDGVPTSIKDILLTKGWPTLRGSKTVDPKG
                     PWNDDAPAVARLREHGAVLLGKTTTPEFGWKGVTDSPLTGITRNPWDPTKTPGGSSGG
                     SAAAVASGMGALTVGTDGGGSIRIPCAFTGLFGLKPSFGRVPAWPLSPFGTVAHLGPM
                     TRTVADAALMLNVLALPDARDWHALPYDGRDYRVGLEDGVRGLRIAYSPALGYARVDP
                     EIAALVKKAAQAFAGLGAHVEEVDPGFEDAQPLFTLHWFPGAAYVVANIPEAKRKLMD
                     PGLLEVARDGAKFSVREIQDAALKRGALGVLMNQFHENYDLLLTPALPLAAFEAGMEM
                     ADIMKQRRWTDWTPFSYPFNLTQQPACSVPCGLTKAGLPAGLQIVGPKHADALVLRAA
                     RAYEATREFSMPPL"
     gene            complement(14436..15311)
                     /locus_tag="AC16O9_08480"
     CDS             complement(14436..15311)
                     /locus_tag="AC16O9_08480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6516689.1"
                     /translation="MSDALPIVDAHHHLWDLSRGPLAYPWLQDPEPHEFFLGDYAKLK
                     RDYLPQDYRRDAAGHEVVKTVHVEAECSREQQVAETEWLTALNAKYGMPHAIVGHAWF
                     HTPNAAEVLERQASFPLVRGIRSKPFRDGMMREKAWLAGYALLERHKLSWDLRVKTWE
                     LEEAAVVVRAHPGIPVVLNHTGFPWDRSPRGLELWRRGMRALAASPHCYCKLSCLCLP
                     DAPWDYESNRRVLLEAIEVFGVERCMFASNFPVDRLRVTFRRMFDDYKRVAQVFTPAE
                     RRALFHDNAARFYRL"
     gene            complement(15308..15979)
                     /locus_tag="AC16O9_08485"
     CDS             complement(15308..15979)
                     /locus_tag="AC16O9_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR6516690.1"
                     /translation="MKRLLVALAFAGCAHAQVPPGAAELAAYTGLHAAAARGEAAEIV
                     RLVAGGADPNARDRYKRTPLHVAAYRSQLGAMRALVKAGANANALEYDRYDIVTIAAV
                     ADDLPVLRLSLELGARAGNTTSRYDGTALIAAAHLGHDEVVRTLIKAGAPLDHVNNLG
                     WTALIESIVLGDGGPRHTATLQALVEAGANVNLADRNGRTPLALARRAGYAEMAAILE
                     KAGAR"
     gene            complement(15984..16778)
                     /locus_tag="AC16O9_08490"
     CDS             complement(15984..16778)
                     /locus_tag="AC16O9_08490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6516691.1"
                     /translation="MPASAFVREGEGRWRATELTRGPWDPQHQHGGPPIALVAGAIAR
                     AAAPLGLTHVARVTANLLRPVPIAELAVAVQTDYAGRNAAHFSASLSAEGKEVARCTA
                     LVQRESDLPLPDGLPGHPLPQAPRAPAESPPSRFPFATGTPGYNDLMEGRVAEGTFFR
                     GPCAVWFRLLHPLIEGEAPLPIERVAVAADSGNGISAILDFRRYVFVNSDLTINLLRR
                     PEGQWICLEARTLFGPSSNGLAEARIYDEQGLVGRSTQSLIVRARE"
     gene            complement(16778..17215)
                     /locus_tag="AC16O9_08495"
     CDS             complement(16778..17215)
                     /locus_tag="AC16O9_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516692.1"
                     /translation="MGHSFLFREGNWRAAGETRDGAGSATAVEGETRVRHETGRWVSE
                     SVMRVKSNPPQEHRNRTEVVPFSAGAQSTHWSGENPSIGSLNGRFVLAGDAILSFYAS
                     ATNRYRGFECMLQKDAKHYAVHGAMLDQDKVVSTWVLELRLER"
     gene            complement(17215..17559)
                     /locus_tag="AC16O9_08500"
     CDS             complement(17215..17559)
                     /locus_tag="AC16O9_08500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR6516693.1"
                     /translation="MIAVIFEFTPAAGRFPEYMQLVEQLKPELEKAEGFISLERFESI
                     TSPGKFVSLQFWKDEASVRKWRNVQLHREAQKKGRAGIFAGYRLRIAGVVRDYTMDAR
                     AQAPQDSVAVHG"
     gene            17594..19135
                     /locus_tag="AC16O9_08505"
     CDS             17594..19135
                     /locus_tag="AC16O9_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6516694.1"
                     /translation="MEPRHFAHWPSDMPRGLALPPLRLHDLLARAASRFPDKPATVFQ
                     GATLRYRELAARVEALAGDLQHGCGVAKGDRVLIDLQNGPDFITALFGVLRADAVAVP
                     VSPANVAAELRHYLEDSGAKVAIAEREVAARFSGLPLERLIVMEDWRPGDKPAAASTR
                     VADDLCLLPYTSGSTGHPKGCMHTHATCLHNIAGSALWKHVTADTVALATAPFFHVTG
                     LIHSFLATVYAGGTIVIQRRWDPLAAAELVERHRCTHWDNVPTMVVDLLSHPGALEHD
                     VGSLAWIFGGGSAMPEAIAQKLFEVCGVRYIEGYGMTETISQTHINPPQNPKKQCLGI
                     PTFDTESLVVDPETFKVLGPGEPGEIVVRGPQLLKGYWGKEMEFRASWAEIEGRPFLR
                     TGDLGRTDEDGYFYISDRLKRMINAAGYKVWPAEVEAAMYQHPAIKECCVIAAPDARR
                     GETVKAVVVLKPGMAASAEEILDWARGRMAAYKAPRQLAFTDALPRTSSGKLMWRALQ
                     DAEFK"
     gene            complement(19214..20269)
                     /locus_tag="AC16O9_08510"
     CDS             complement(19214..20269)
                     /locus_tag="AC16O9_08510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HIR6516695.1"
                     /translation="MKAFTYTSLPSRVVFGAGSVARLAQEVDKLGAKRALVLSTPGRS
                     ADVKRLAASLGARYAGLFDKAVRHVPIEVAEDARAAAKAANADCCIVVGGGSTIGLGK
                     AIALTTGMPVLAVPTTYSGSEMTWVQGFTEGGRKRTQFDPKMLPKTVIYDPELTLGLP
                     AATAAASGMNAIAHCVEALYAKEANPVVSLIAEEGIRALAASLPLIVKNPRDLEARSE
                     ALYGAWLAGISLNTGIALHHKLCHTLGGTWNLPHAETHSIILPHAVRYNRDAAPEAMA
                     RIAAALGATDAAGGLYDLEVRLGLQMKLADLGVPEADLERAAKIAMEAPYPNPAPVTY
                     EGVLALLRNAHAGLRPK"
     gene            complement(20266..21441)
                     /locus_tag="AC16O9_08515"
     CDS             complement(20266..21441)
                     /locus_tag="AC16O9_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6516696.1"
                     /translation="MPGRAVLLLSLAAFASAAALRAADPLLPLIAGEFGTTPGGAAAV
                     ITGFAVAYGLLQLVNGPLADRLGKYRMVALITAISAAGNLACALAPSLATLVVARFLT
                     GATVGGIVPLAMAWIGDAVAYDRRQLVLARFLVGHMLGVAFATTASGFLGERYGWQAV
                     FVLLALLYAATALLLLLELRGNPQTRAQPIDATPLAEAFRRMAGLTRLPWVRVILATV
                     FIEGALFYGALAFVALYVHQRFGLGLGASGAVSASFAAGGLLFAASAGRLLPRAGEGG
                     LVLGGGGLMAVGLLALAAAPGPGWAVPCVVVAGAGIYMMHNTLQVHATQMAPATRGAA
                     LAIFACSLFTGQSVGVWLASHAVDAGGARWMFAAAGIGLLLLALDFRRRLAKLRRSA"
     gene            complement(21459..22820)
                     /gene="mgtE"
                     /locus_tag="AC16O9_08520"
     CDS             complement(21459..22820)
                     /gene="mgtE"
                     /locus_tag="AC16O9_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6516697.1"
                     /translation="MAEVIDTPPWERLEALAQARDVRALEAYVESIGPSEAFRALLHL
                     APEARERVLTTLSPEEAADLIEEIPEEHAADLIEELEVKDAASIVSEMQSDEQVDVLA
                     NLDPQDAEAILARMPPEEAAEARKLLPYPRDVAGGLMVTEYLSYADTSTVRDVIEDLN
                     RRTEEGLAEDLHAYVVSGAGRFVGALDLRDLVLARRDTLLAQLLRPAAHVRPDATLDE
                     LEAYFDREAVAAVPVVDARQALLGVVRRGDVAEEVAARAENDHRKMQGIVGGDEIRSL
                     PVMVRSRRRLSWLSVNIILNIIAASVIAAYEETLAAVIALAVFLPIVSDMSGCSGNQA
                     VAVSIRELSLGITKPFELLRVWLQEVKVGLINGVVLGALLALAAILWKGNPWLGLVVG
                     GALAANTLVAVSIGGTVPLVLKRLKLDPAVASGPILTTVTDMCGFFLVLSFAAAILPR
                     LVA"
     gene            22885..23550
                     /locus_tag="AC16O9_08525"
     CDS             22885..23550
                     /locus_tag="AC16O9_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR6516698.1"
                     /translation="MPALPDPHALAIMALTVFALVLFAQDRIALETSSLLVFITLVAG
                     FQIFPYAGADGPLRPRELFAGFGHEALVAVCALMVLGKGLETTGALQPVARILARVWQ
                     TGPAVSLLFTMLVAAALSAVVNNTPIVVMLLPILVGVTLSAKASPSGVLLPFGLATVI
                     GGTVTTIGTSTNLLVVSVARDMGVRNFGMFDFALPIALVGFFGILFLWLVAPRAAAAG
                     ARR"
     gene            23505..24764
                     /locus_tag="AC16O9_08530"
     CDS             23505..24764
                     /locus_tag="AC16O9_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6516699.1"
                     /translation="MAGGAARCGSRRAALISDKSARLFSAMLYVEDDSKIAGKTLADA
                     RKLAGGNLRLLDVQRGERGVLLARLPSLTFMPGDRLLVRDTPERLKELEGMLGTPLHD
                     QETESAEEREEKEERAAAAEGEPKATAEEDAKQRLVEVVVTEDSPLNGSTVRRSRFAD
                     EYGLFVLAVHRGQFRDEIRRGELGDVVLSTGDVLLVQGSVGDIKRMKSNQAFLVLDST
                     VELPDTERAALALGIMGAVIAAAATGLLPISVSALVGVAAMMATRCLTWREASKGLSA
                     QVIMIVVTSLALGTALTRTGATAYVAELFVAATAGMSPSAILGALMLLMAVLTNILSN
                     NAAGIIGTPIAIGIAARLGLAPEPFVIAIIAGVNLSFATPMAYQTNLLVLHAGGYRFV
                     DFVKVGVPLSLLMWAGYLYVIPLFFPF"
     gene            complement(24772..25899)
                     /locus_tag="AC16O9_08535"
     CDS             complement(24772..25899)
                     /locus_tag="AC16O9_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6516700.1"
                     /translation="MTDQTLALLRDTLRRYVEKEVVPHGATWEAQGCVPREVLRQMGR
                     LGFLGLRYAEEYGGGALDARATALLAEELGRSTFGGFAITVLVHTDMASPHLANAGTK
                     EQIATYLPKIVSGEAITAVAVTEPDAGSDVKAIRTSARREGGAWVLNGAKMFITNGAL
                     ADLYFVAAKTEPAAGAKGVSMFMVEKGAPGFTVGRTLDKMGWRSSDTAELVFENCRIP
                     AANLLGEENRGFYAIMKNFQNERIAIAAMCMGEAAKAIELTLEYVRARQAFGAPLWDK
                     QAIRQRLAMLAARVEAGRQLVQHAAALDADGRDCVKEVSMAKAYCAELLNEVVYDCLQ
                     FHGGFGFIRESAIERMYRDARVQSIGGGATEVMLEEVAKRL"
     gene            complement(25896..27404)
                     /locus_tag="AC16O9_08540"
     CDS             complement(25896..27404)
                     /locus_tag="AC16O9_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6516701.1"
                     /translation="MSGWAPRVLGEFLALQPPRAEALVTPTRRLDYGALHAAAHSVAR
                     AMHGLGLRKGDHAAILMGNDQHWLALFYGAALLGVVTVPVNTRFKAAELEFCLKQADC
                     RAIFAAPGFRGNDYAAMLREIAPRLPLLEHKVLLGEDSWRGFAALDHGQKIAWDVRPD
                     DLLLIQFTSGTTAYPKGVMLTHDNMLRNAWAAGERIGVKAEDRYFNCRPLFHVGGSTL
                     SALMCLVHGACLVTLPTFEAGAALAMMEAERCTLTSGNDTIFQLLMGHADFPRRRLVL
                     RGGWAAAGPQTLQRIIRELGAKRICAAYGLSEASPNVVMSDWRDAEALRVQGLATPLP
                     ELQLRTLDGEIQVRGWSVMRGYYNDPEATAQAFTEDGWLRTGDLGELHAGGRLRMLGR
                     LKDVFRVGGENVAPAEVEEVLLGHPAVQSAQVVGVPDARLGEVPCAYVSLKAGHKLTE
                     DALLAWVRERCANFRVPRYLRIVRSFEAIGMTASGKVQKNRLREQALREFKL"
     gene            complement(27401..28114)
                     /locus_tag="AC16O9_08545"
     CDS             complement(27401..28114)
                     /locus_tag="AC16O9_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6516702.1"
                     /translation="MTVLVERRDGVAILTLNRPEARNALNLAMCDALMAAAQGLDDAV
                     RLVFVRGRGPVFCAGADLKERQGMSAAQVRERRMKAFAAYAALESLPMPAIAVVEGAA
                     VGSGCEIAAACDFIVATPQASFRIPEAIRGTVGATQRLPRVLGKRLAKDLMFTGRTLS
                     ADEAERHGLVARLVPADRLESALAEIAKTIREAPARTLALAKRAIDRGVELDPKGALE
                     TELAAIEAQLAAGDWMGRA"
     gene            complement(28111..29286)
                     /locus_tag="AC16O9_08550"
     CDS             complement(28111..29286)
                     /locus_tag="AC16O9_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6516703.1"
                     /translation="MKPLEHLTILDLSRVLACPFASMILAELGATVIKVEQPGAGDET
                     RGFEPQLAKDSAYYFACNRAKASITVNLRSDGGRRIIRELARQADALVENFPVGTLAR
                     QGLDYPALSSLNPKLVYVSCTGFGQSGPYAQKKGYDTVFQAMGGLMSLTGEKGGGPVK
                     PGLPLADLTSGLWVAIAMLAALAGRERTGRGAYVDFSMLDGQVSLLTLAAARYFALGE
                     VPPRLGTEHPGRVPSATFRCKDGKYAHITASDQHWPKLCEALQMGTVAIYGTNAARVA
                     RRDEVMAAMGAAVAKLTRAELIASLDAADVPVGPVNDVAEVLADPHVRARQLVGSFDY
                     PGVGEFKALALPYKFLGWDDPQIGPPPALGEHTEAILKERLGYSDARIDELRKAKAI"
     gene            complement(29288..30175)
                     /locus_tag="AC16O9_08555"
     CDS             complement(29288..30175)
                     /locus_tag="AC16O9_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6516704.1"
                     /translation="MGITYALLAMLAFATNIVVTRFAVARMPVEAGFFIVLATNIVFP
                     ALLFAVELGVRAAPYGWSWKGAGLFAIGGVIGTFLGRRFLFDTVKLLGPSRASVFHST
                     APAFALVAAWLLAGELLGPYELALMVLVWIGLWLTQPRTGSLHALAPEVLRKGFLAGL
                     LTVAGFGFGNVLRGLAMREWSEAALGTVIASVAALACQVVVTHQWGRVAAQLRAADRT
                     ALLLYTGCGVLTSLGSIFIALAMTQIEIALAVLVVHTTPLVIFPVSVWILKNREELTG
                     RTVSGAALVLVGIAALAFR"
     gene            complement(30179..30904)
                     /locus_tag="AC16O9_08560"
     CDS             complement(30179..30904)
                     /locus_tag="AC16O9_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6516705.1"
                     /translation="MSVLLVKDQGAVRVLTLNRPDKRNALNTELTQALLDALRTADAA
                     ESVGCVVLAGAGPAFCAGADVTEFAGLTPEHKAKVEARAELTMQLHLAIPKLAKPVVS
                     AVHGAALGGGAGLAIAGDLVVMGDGAALGYPEVRHGIVAAIVMANLVRQVGRKAAFEL
                     VALGEPVGAARALALGLVNRVVPRAQVLKEAVALADKLAALGRPALRLTKQLFHEVAD
                     LPLEQALARGREANKRMRAFRKP"
     gene            complement(31023..31508)
                     /locus_tag="AC16O9_08565"
     CDS             complement(31023..31508)
                     /locus_tag="AC16O9_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6516706.1"
                     /translation="MKPRSSTTSAPGIGALLSYRLHVVANLLSRGAELRYRREFGVSL
                     WEWRTIALLGGVAEPQSLNDLASAAGIDKSQMSRVVSGLAARRLVLRSIDAEDARGVR
                     LTLTRAGRALYQGLIRAAAERNDAFLDCLGARERAAFDAALEKLAARARELIAREREK
                     A"
     gene            complement(31483..32037)
                     /gene="pcaG"
                     /locus_tag="AC16O9_08570"
     CDS             complement(31483..32037)
                     /gene="pcaG"
                     /locus_tag="AC16O9_08570"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HIR6516707.1"
                     /translation="MNIPTGEATLGPFFPPRYVDAGASDLTQLAGRKARGEAIEIVGC
                     VRQEDGTPLDNLIVEIWQADAQGIYRHPADPRHAEADPDFLGWGRAATDKAGHYAFRT
                     IRPGAPEGRAPHLSVMVMFSGLMRILRTTMFFPGDPANDADPALAAVPAARRALLVAR
                     EEAPGRYRFDLKLRGEHETPFFDD"
     gene            complement(32034..32732)
                     /locus_tag="AC16O9_08575"
     CDS             complement(32034..32732)
                     /locus_tag="AC16O9_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR6516708.1"
                     /translation="MQVVITPRDERTQPTRIVPGYPQTPWRNPTQPLVRRAPTLSELT
                     GPLQVARRVQLLGGDIAGRGEKQAIGQLMQLRLSVVDEDGAPVGGSVVEVWQANAAGR
                     YIHPNDDDHAPVDVNFYGAARLATDAGGLLELRTVKPGAYPVPTNDGWWRPPHIHFSV
                     FGKVWLQRLVTQMYFPGEPLNAQDRIFNAVPDVAARGRLVARLLPPTGEPRNALVYEH
                     RIVLRGRRATPSQP"
     gene            32772..33671
                     /locus_tag="AC16O9_08580"
     CDS             32772..33671
                     /locus_tag="AC16O9_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6516709.1"
                     /translation="MAAVTLCECFARDGLQHEPAFVPTAVKRALIERFATLGFPRIEA
                     TSYANPKVVPQFADASELLQALPRRPGAFYKATCANPKAVERALADLGAGFGANEVSL
                     LVSASEAHTQKNLNRTRAEQWANVEAMVRLAGGRFRLVGTVSVAFGCPFEGKVDPGVV
                     AEDVARFVALGVTHVALGDTTGLATPKAVKAMVARLPAGATPIAHFHDTRGTGLVNYV
                     AAFEAGIRLFDSSFGGAGGHPAKLQYGEGHTGNVCTEDLVTLFESMDVATGIDLDGLL
                     ETARFCEAALGRELHGRVTRLVG"
     gene            33692..34807
                     /locus_tag="AC16O9_08585"
     CDS             33692..34807
                     /locus_tag="AC16O9_08585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6516710.1"
                     /translation="MKLGYFTMPLHPPGRPWVETLREDREAILLCDRLGYAEAFVGEH
                     ATDAAESITSCATFLASLVHDTKRIKLGTGTLNLSNSHPVTVATTVAMLDTLLEGRFI
                     MGISPGGLPSDMEVFENLGKDRGKIFLECINHVLAIWAGEPPYDIKGEIWNVSTAKTQ
                     ILDIGQGVMLKPYQKPHPPIVVTAVEPFSKGVTAAAARGWDPISANFLLPQWVKSHWG
                     RYAEGCKEGGRAADPANWRVAKTVFVADDAAKAKAYGRGAQSPYRFYYSQLLTKLKRA
                     GRANLFKHAKDAPDDSVTLDGVMNDLVIAGTVNEVVDRLLAFHAQAGDFGTLLYCGID
                     WADAALARRSLELMAEEVMPAVNNAIGGKETKRASAL"
     gene            34791..35672
                     /locus_tag="AC16O9_08590"
     CDS             34791..35672
                     /locus_tag="AC16O9_08590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6516711.1"
                     /translation="MRLLSSRLPYSAIVDRPPLKLPQGGRLIVWTIVNVEEWSIERPM
                     PRTVLSPPLGQPLLPDLANWAWHEYGMRVGFWRLLEALQRLKITPTLAINGSVCKTYP
                     RVAQAAHEALWEFMGHGYVQRPMHHLENQRQAIADTVAAIRECTGKAPRGWESPGLSE
                     TPETLDLLAEAGFEYVANWPADDQPCRVETAKGTMVALPYTLEMNDIPMMVAQGHPSE
                     EWLRRGMEQFDRLYAESERITRVMAVSVHPYITGVAHRIGYFEKLYQHMARRPGVLHW
                     TGERICDWYVSETQKGN"
BASE COUNT         5472 a        12723 c        12230 g         5331 t
ORIGIN      
        1 gaccgcctga cgcgcacgcc cgcggtggtg cacgtggtcc ccgggcaggt gatcgagatt
       61 cccgtggcgc gggacgggca ctactatgtc gagggctccg tcaacggcac acccctgaag
      121 ttcatgatcg acaccggcgc gacctacgtt tccgtgagcg gcaagttcgc ggccgcggcg
      181 cgcctgcccg agggcgtgac gggctacttc agcaccgcga acggaactgt cgagggcaag
      241 atcgtcaagg accaggtggt ggaggccgag ggcttcaagg tgagcggcct cagcgtcgcg
      301 gtgaccccgc tcggcagcaa gcaggggctg ctcgggcaga atttcctcaa gcgcttcgag
      361 gtgtcgcagt cggcgggcgt gctgcgcctg cggcaggcgc aatgaaaaag cagttcgaca
      421 tcccatgaag ctgcgtagat tcggccgcac cggcctgcag gtgtccgagg tggtgttcgg
      481 cgccggctac gtcggcggca tcatcgtgct cgccgacgac gacacgcgcc ggcgcgcgct
      541 gcgccgcgcg ctcgacgccg gcatcaactg gatcgacacc gcgccgctct acggccaggg
      601 gcgctcggaa gaggcgctcg gctggctgct ggcggaagtg aaggagaagc cctacctctc
      661 gaccaaggtg cggctggacc tcggcaagct cgccgacatc ccgggcgaga tcgagcgcag
      721 cgtgcacgag agcctgaagc ggttgaaacg cgattcggtc gatctcctgc aactgcacaa
      781 ccagatcggc gcgaagaccg gcagcggcgt gctcggcgcg gtcagcatcg acgacgtgct
      841 gaagaagggc ggcgtcgccg acgggctcga gcgcatgcgc gagcagggcc ttacgcgcta
      901 catcggcctc acggcgctcg gcgaagcgcc ctcgatcatc cgcgcgatcg acaccggccg
      961 cttcgattcg gcgcaggtgt attacaacct gctcaacccc agcgccgcgc gtgccatgcc
     1021 gaagggctgg agcgggcacg acttctccgg cgtgtgcgcc gcctgcaagc gccacgacgt
     1081 cgccatgatg aacatccgcg tgttcgcctc gggcgtgctc gccaccgacg agcgccacgg
     1141 gcgcgagatc gcgctcacgg agaacagcgc actcgaggtc gagaccaagc gcgcgcacgc
     1201 ggccctcgcc gcgctcggca ccggccacgg cacgcgcgcg cagaccgcgc tgcgctttgc
     1261 gctcgccaat cccgacctcg cctgcgtggt gatcgggctt gccgagctgt cgcatctcga
     1321 ggaagccatc gcggctgaag ccaaggggcc gctgccggcg gacgtgatcg agaagctcga
     1381 gcccgtgtac gctcgcggct tcgcctaatc gaatacagag gaggagaaat gcccaagacc
     1441 gtactcatca ccggcgccgg cagcggcttc ggccgcgccg ccgccatcga gctcgccaag
     1501 cgcggccaca aggtgatcgc cactgtggag agcgacgcgc aggccgccga gctctccaag
     1561 gcccacccgg aactcacggt ggcgaagctc gacatcacca acgccaggga cgtggcgacg
     1621 ctcgacaaat gggacctcga cgtgttcatc gccaacgccg ccatgggcca gaccggcccc
     1681 ctgtcggtca ttcccctgga gcgcctgcgc aaggtgttcg aagtcaacgt gttcggcacc
     1741 ttcgcctgcg tgcagcgtgt cgcgcagaag atgcgcaaga agcgctcggg ccgcatcctc
     1801 atcgtgtcct cgatcgccgg cgtgcgcgcc ggcgtcggct cggggcccta cgcgatgacc
     1861 aagcacgcct gccaggcgct cggcatggcg ctgcgcgcgg agctcacggc gttcaacgtg
     1921 gacgtggcgc tcatcaaccc cggcccctac gccaccggct tcaacgaccg catggcgaac
     1981 gaccccggag agtggttcga ccgcaagacc gcgcatcccg aggacgtgaa ggcgatggat
     2041 tcgctcgtgc agcgcatcac cgtcggccag atggacccga aggaggtggt gcagcgctac
     2101 gtcgagctgg tcgaggccga ccagaccgag ctggtgaact tcatcccgcc ggacatcgtg
     2161 gagcgcatga gccggccgaa gccggcgcgc tgagcgcggc tgctacggac tgatcttgcc
     2221 ctcgccctcg aggccgaact tcgcggcgat ctcggtccag atcgcgggcg cgcagttcga
     2281 cttcacgatg ggcgccagcc gtcgccactg cgccggggag caggcgtgct gcaggcccca
     2341 ggcgaacacg cgtacggcga tgctggccgg ctgcgctgcc gtgccttggc cgctcagcat
     2401 gcgcaccacc cagccgatgt accagtcgaa gtccggcagc ttttcgctcg gggcgagcac
     2461 ccggtcgtga aaaacgcgcg cgcgcgcttc gggcgtgggc gccgtcttca tggtcaacgg
     2521 catcatcacg tcttcctcgt gcgtgaggtg atgcagatga tcgtctcgcc atgccggcca
     2581 ggcgctgcgc agcgcgccca ggtcgttcgc ggcgagcgcc gcgtccaccg ccctggcgag
     2641 cctcaggtcc tcgacgtgct cggccggcac gcccgcggcg ctgatggcgc cggcgccgac
     2701 ctcgtcgagc aaggcgaaca tgaacttgtc ctccatggcc atgtgcacgg caagcccgcg
     2761 ctggaacgtg cgccattcgt cgccaagccc cttcgcgtcg cccgcctcga gtttctcctc
     2821 ctgcagtcga acgctcgctc gcagggcctc gtgcgagttg cgcatgaccg cgaacggcat
     2881 gggcttgggc gcctctgcgc cgctggactg cgcgccggga ccgggatcgg gcatggctta
     2941 agcttaaagc gattcgcttg cgcagtcagc caccttgcgt cgccgggcgc cggctcagga
     3001 catcgccccg ccgtcgacgg tgtagatgcc gccgttgacg aacgacgcct cggcgctgca
     3061 caggaacgcg accagccccg ccacctcctc gggcctgccg tagcggccca tcgggatgcg
     3121 cgccaccgag cgctcgtgcc cggccttggg gttttgcggg tccaggcccc tgtccagctg
     3181 ctcgcccatc ggcgtctcga tcggccccgg gcagacggca ttcacgcgca cgccctgctt
     3241 caccagctcg atggacgccg tgcgcgtcat gccgaccacc gcgtgcttgc tcgcggtata
     3301 ggcgaagagc ccggccgtgg cgcgcaggcc cgcgatcgac gccgtgttga cgatggcgcc
     3361 gccgccgcgg cggatgatcg ccggcgcaac gagcttcaac cccatccaca ccgccttcac
     3421 gttcacggcg aagacgcgat cgaaggtttc ctccggatac tcgagcagcg ggcgattgac
     3481 gccgaggatg cccgcgttgt tgaagaagca gtcgacgccg ccgaactcgg actcggcgcg
     3541 ggcgacgtag ttttcgacgt cggccttgcg cgtgacgtcg gcttccaccg ccatcccctg
     3601 cccgcccgct ttcttgatcg ccgcaacgca gccggccagg tcggagcccg gcaggtcgac
     3661 ggcgacgacg cgcgcgctgt cggcggcgaa gcgcatcgcc gcggcacgcc cgatgccctg
     3721 cgccgcgccg gtgatgacga tgaccttgtc ctggaaccgt gccttcatgt tcactccttt
     3781 ttcgctgcgg ccacgtgcag cgccgcggtg cggccgaaca ccgcgccgcg cagcaccgac
     3841 gtcgagccgg tatacacctg atggtagagg cccacggtct cgcccgccgc gtacaggccc
     3901 gggatcacct tgccgtcgct gtcgagcacc tgcgcgtggg tgttgacctt cagcccgccg
     3961 aacgtgaagc agttcgccga gatgatgggg taggcgcgaa acggcggctt cacgatcggc
     4021 cggcaccagt gcgacttcgc cggcacgagg ccgcgcgccg cgcgcccgtc cggcgcgaac
     4081 ggctcgaact cgcccgcggc cggcgggcag gcgcggttga acgccgcgat ggtctcgagg
     4141 gtttcggccg gcaagccgag cttctccagc agcgcctcca gcgtcgccgc ctcgtagggc
     4201 ggcacgtcgg agcggatgct cgtcttccag cgcgggatct cctccacctg agcgtcgaag
     4261 atcacccagc agatgccgtc gggctggtcg gcgacggtac gcgtgatcgg gtcgtaccag
     4321 gcgtcgacca tgcccgggcc ctcgttcacg aagcgctgtc cgagcttgtt gacgaggatg
     4381 ccgtaggggt agatgtgcac caccgcctcg ggctggcgcg agcgcggatc gaccggctcg
     4441 gcgtggtacg agccgaagtc gcctgccggc gccgcgcccg cggcgagcgc catgcggatg
     4501 ccctcgccct tgttgtagta gccgccgcgc gccaccgggc ggatgtgcgc ggcgcgggtt
     4561 cccatgtagt gggtcatcat ttccgggttg ccctcgtagc cgccgctcgc gagcagcgtg
     4621 gcgcgcgccc ggatctcctt gcgctttccc gcctcgtcga tcgccacgat gccggccacc
     4681 cggccgtcgg ccgcgcgcac cagatccacc gcggtggtgc ggaagtgcac ctcggcaccg
     4741 agcgccttcg cttgctgcat cagccgctcg atcagcgcga gacccccgcc ctgcgcggcg
     4801 atgcgcgagg tgctctgcgt cagcaggtag atcggctgcg gcgcgaagcg caggccgaag
     4861 cttttcagcc aggcgatcgt cggcggcacg ctccgggcga aatgcgcgat cagctccggg
     4921 tcgggcagcg ggtgcgcctt cacgtacgcc ggccagtgcg cgtattcgcc ggccaccgcg
     4981 tcgagcacgt tcgggtcgag gttggcgccc gcgttctcgg cgaacttctc ctcgaaatcg
     5041 tcggacacct cggcgtcgtt cttcatgcgc aggtaggcct cggtccagcg cgtgttgccg
     5101 ccgaagtcct cttccggggc gcgctcgagg agcgtgactt ggaggcccgc ctgcagcgcg
     5161 ctgaccgcgg ccgacatgcc ggcgatgccg cagccgacga cgacgaggtc gcggctcaca
     5221 ggatctcgaa cacgtcgtag aacaccgcgc tcggctcgcg gcggccgacg cccacgccga
     5281 cgatgcggtt caaccagccg tacttcttcg aggccgtctc gaacacgagg ctcgtgcgca
     5341 ggtagtactc gcccggcggc acgcgctcgc ccttgccgat gcgctgcatg acctcggccg
     5401 gcgcgtggcg cacgccgcgg tactggatga gcaggatctc gccgtcgtcc agcttcagga
     5461 tcaggcgcac gtcgggccgc acggcgccgt ccgcgccgcc gagcaggatg tccatgccgc
     5521 cggtgagcat ctcggcctgc actttcgggc ccttgaagtg gcccttgtcg aggcggtcga
     5581 gccggcgcct gccgaccggc gcgtcgccca gcatgtagct gccggtgatc tccatcatca
     5641 gctcgccgat gtacgcggat ttgagttccg gcattgcttc ctccgtcagg ttttcgtcgc
     5701 cagcacgccg tcgccgcgca gttccgcggc gaatccggcg ctgcgcaggc gcttggccac
     5761 ttccctgggc acgtcgcgtc cgctggtcag cttgttcggg gagccagtgt aatgaaacag
     5821 cagccccttg cgcttgagca cgcgcgcgag ctcatcgtag aaggcctgcg agtacagctc
     5881 gccggcgatg ccgaagcgcg gcgggtcgtg caggatcgcg tcgaccgagc catcgggcag
     5941 cgtcgggagg tgctgcgcga tgtcgccgtg ggtcagcgcg agcgcgcccc cggcctccgg
     6001 cgaccaggga ttgaggccgc gcagccagat cacgtcttcg ttcttctcgt aggaaagaac
     6061 ctgccgcgcc ccgcccttca ggcacgcggc ggcggtgtag cccagcccgc cgcaggtatc
     6121 gaggatcacc ttgccgcgcg gcctgaccag gccggccttg cgctccgcgt ccacgtaagg
     6181 cgagatgtgc tccgtgggat gcatcttgat gccgtcgatc tcgaacgtcg gcgggcccca
     6241 gtgcgtgggc accagcttga cgagcgacgc ggtgaaccgt tgcaccggct ggaaggcatc
     6301 gtccacccag tagtagatgg tccgcgcctt gcacgattcc aggtacgcga aacgccggcc
     6361 ctgccagcgc catccgtccg cgtccacctc gacggtcgtg gtcgagcggc ccaggtcgag
     6421 cgagcattcc accgccggct tgccggcccg cgccgcgttt cgcagcgcgt catgcacctc
     6481 ccgggtcagc agcggccccg ccattcccga ttctgtatca tggaatcgag gaggcttcat
     6541 gtcgctcgcc gcgcagccga tcgcgcagtg ccggcgggac ggctacgtca gccccgttcc
     6601 cggcatgccc gcgtccgagg cggggtaaag gaggccatcg ttccgtttca agccactaca
     6661 atgcacaaag gagtcactca tgggcaacat cgttggcggc tgtctctgtg gcgcaattcg
     6721 ctacacttcg aaagcccaac cgctcttaac tgcagtctgc aactgcaagc actgtcaacg
     6781 tcaaactgga accgcctttt ccgtattggt ggccctcccg aaaggaacac tcgagttctc
     6841 tgggaaccgg cctgccacct atcacgatac agggagtagt ggtctgccag tgctgcgaca
     6901 cttctgcgcc aattgcgggt ctccaatctt ctcggacgtt gctgctactc cggcgatgga
     6961 ttggctgaag gctggaactc tcgacgacgc gtcctggctt caaccgcaag tcagcatttg
     7021 gtgcgactca gctcaacctt gggttcaaat gagtgaaagt atcgcccgtt ttcctcagaa
     7081 ccccccacca gcggcctaac cattcgctca agccgacccg ctccggcctg cggcctgcgc
     7141 gggcggctta gctcaaacgt taggcgtcac atttgcatcg ccaagcgcac ggtcaccacc
     7201 agttatcaac taccggttgt cccgcagcat ccgcgggtcc acaatcctca cccgctctgc
     7261 gccgcgcggg gtggaagatg tttaggcgct cgttctactt gtccaaggag acggaaatgc
     7321 ccaagtacat gcttcgagtc aactacaccg ctgacggtgc gaagggcctg atcaaagacg
     7381 gcggctcaac gcgtcgatca atcgcgcaga aggccgcaga gtcggtcgga ggtcgcgtcg
     7441 agtcgttcca cttcgccttc ggcgataccg atgcctttgt aatcgccgac ctgcccgacg
     7501 caacaagcgc tgctgccatt gccctgacgg tcagtgcaag tggcggagcc agtgtccaga
     7561 cgaccgtgct gatgacacca gaagaggttg atgccgcagt acgcaagtcg cccttgtact
     7621 ccgcaccagg tcgctaaacg ccagaagccg cagctgctct tggcgtcacg ggggacaggc
     7681 ggacacagcg ttgacgccca ccccatcgct caaacggagc gccaacggca ggccaccagg
     7741 cccgggcctg gtggcctgcc gtcgtcgccc gcttagctcg cacgttagag acggcggaat
     7801 ctcttgcaag ccttcatcac ctcggccttg gttgtcgcac tcgcggagat gggcgacaag
     7861 acccagcttc tgtctttctt gctcgcggcg aagttcaggc acaggatgcc gatcattctg
     7921 ggcattcttt tcgcgacgct cgccaatcat ttcctcgcgg gctacgtggg ggtctggctc
     7981 gcgagccttg tgtcccccca agcgctgaga tggattgtcg ccgtctcgtt cttcgccttc
     8041 ggcgcgtggg cgctgaagcc cgacaagctg gaggaccgaa agctcggagg cgccggcgtg
     8101 tttgtgacga cactcatcgc cttctttctg gttgaaatgg gcgacaagac ccaactcgcc
     8161 acggtggcgc tggcggccca ttacgatgcg ctcgtcccgg tggttgtcgg gacgacgctg
     8221 ggcatgatga ttgcgaacgt gccggctgtc tggatcggcg aagcgctcgc ccatcgggtg
     8281 aatatgacgg tgatgcggtg ggtggctgcg gcgctgttcg ctctactcgg cgtcatcgtc
     8341 ttgattgcgc cgactccggc tgcgtagcgc ggctgcgccc agacccctac gccacccact
     8401 ccgacacggg cgccttcgcc tcctgcagcg gctggcacgc cacgtccacc agcgtaggcc
     8461 cgtcgaacgc gaccgcctcc ttcagcgccg cacccagctg cgccggatcc tcgacgctcc
     8521 agctcttcac cccgaacgcc tgcgccacct tggcgtggtc ggtcacgccg aaatcgaccg
     8581 agaagtaccg ctgcccgtag ccggtcttct gcccggcctt gatccagcca tagaccgcgt
     8641 tgcggatcac gaccaaagtg atgggcagct tgtagcgaac caaagtctcc agctcccccg
     8701 cgcacatgcc gaacgacccg tcgcccatca ccgcgacggt cttcaccgac ggccgcgcga
     8761 aatgcgcgcc caccgccgcc gccagcgaat agccgagcgc gccgtgcgcc cggtttgaaa
     8821 acagcgtgcg cccggctttg cgcaccgggt agtaggccga caagtaggga cagggcgtcc
     8881 cgggatcggc gacgacgatc gcgtccgcat ccaggacctg cgacagcgcc tgcactaccc
     8941 gctccggctt gatcgggcgc tcgccggaag acgccagggc ctcgaatttc gcgagcttcg
     9001 ccgccttggc ctttgccacc gcctcggctt ccaacggccg gcgcaggtcg tcgagcgccg
     9061 cattcagggc cgcgagcgcg agccgcgcat cggcgaccag cggcacgtcg gcgacgtaat
     9121 tggtgccgat ggtctgcgcg tcgacgtcga tgtggaggat cttcgccttt cccggcaccg
     9181 gatgccgcca gcgctcggtg gtcaccgacc ctgcgcggca gcccacgaaa accaccaggt
     9241 cggccgtatc gacgatggcg cgcgtttccg gcgtgccgcc gttggacccg accaccccga
     9301 ccgccagctc gtgctcctcg gagatcgagc cctggccgct gatggtggtc gccaccggcg
     9361 cggacagccg catggcgagc tccagaagct cttcctgggc gcccgagatc accgggccgc
     9421 cgccgcaaat gaacacgggc cgcttcgccg cgcgcagcag cttcgccgcg agctcgacgt
     9481 gaaacgggtc gggcgcgatg cgccgcgacg gatacgaccc cagggacgga tcggcccaga
     9541 cgtcgccccg ctccaccggt gcgttctgca cgtcgaacgg cagggcgatg tgcgccgccc
     9601 ccggccgccc cgtggtcatg gcgttgaagg cggcgcggaa cacgcgcggg atgtccgccg
     9661 cacggtccag caccgcattc cacttggtca agggccgcat cagcgccttc tggtcgagct
     9721 cggtcagcgt gaagcgcccg cgcgaggaca ccgacacgtc ggtgttgatg ccgagaaccg
     9781 gcaccgagga ctcgttggct tccacgagcc cgggcaggag gtacgtcgcc ccgccgcccg
     9841 agggcccttc gcacacgccg accttccccg aaacgcgcgc atagccgtcg gccatgtagg
     9901 ccgcgtggcg ctcgtcgcgc gtcagcacgt gcttcatccc atggggcagc cgcgccagcg
     9961 cgtcatacaa aggcaggctg gtgtcgccgc acaggccgaa aatcacttcg acgccgtgga
    10021 cgttcaacat caccgccacg gcctcggcgc cggtcagcgt cgcggcgccc gcgtcgattg
    10081 tcttgttcat gcagtaatgg tagcgcgcca tgcgcgttgc cgactgccag cgcccgccgc
    10141 agcggcgagc gcatggccga gcgcggcggg ctgcgtggct atgatcggcc ctgcggagat
    10201 tgctgcagag cgcacgcctg attcatacct aatgcacaag ccatgcccag ctgttccgcc
    10261 gcctccctct ggtagccgct gtcgagaaga tactgtccga actggaagcg gatgttggcg
    10321 gatcgcggat ccaattgcac tgccgcgcgc aaatccttta acgcagccag cgaacttccc
    10381 gactgccatt gcaacgttcc gcggcgggca agcgcgtagg cggccatctc cgcgggcccg
    10441 ttcttgcgcg cgagcgcgtg cccgcgcgtg aaatcggcca tcgcctcctc gcgacgtccc
    10501 agcgctgcgt ggacgttgcc gcgattgacg agcgccgacg catagtctgg atacaagtgc
    10561 agcgcccggt tcaggtccgc gagcgcctcg ggccatctct gcgcgcttcc gtgcatggca
    10621 cctctgttgg cgaacgcacg ccacgctccg ggcagagatt cgtctgattg ctttgctacc
    10681 gcatcgttcc agagcgcgaa ctcgctgctg aagctcgtca gccggccggt cgcgcccagc
    10741 gccagcagca gcactgccgc cgcggcgagc gcgaagcgca ccacgcggcc cggggcgtac
    10801 gcggcgacgg ccggcacgag ggcgcatccg gtcatccaga ggtagctgcg gtacaggacg
    10861 aacggttcct gaatgcgcac gacggtgaac tcgacgcaga acagaagcca cgggaacatc
    10921 agacccagcg cggccgcagc cgccagtccg ccgcgccgca ccagaagcca gcatacgacg
    10981 ggatacgccg caaaggcggc catccccgcg actccgccca gtccggcgaa taccgtggcg
    11041 aagggccgac gcaaatccac cgcaagccat tgcggcagag gaagcagcca gatcttcaga
    11101 taatcgaaga acaatcctgc ttgcgtctgg atgctcagaa gatgggcgtt gtcgacgtcg
    11161 acgcgggttc cgcctctggc cagatgctcg aagtatctca tcgcggcggg ttcgtacgga
    11221 gcgccgagca gtcctttcac gcgcagggtc acgaacaggg cgatcaatcc gtaagcgacg
    11281 aacagcgccg caagctcgcc ctttgcgcgc ctcgccgcag gtcctgccag aatcacgatg
    11341 agcgccgcca ccgcaggaag cgcgacggcg tgctccttgg agaacaccgc cagaagaaaa
    11401 aacaacgcac tgcacgccag tgaccagcgg cgtctctcga tgaccccatg cgcgagagcg
    11461 atgagcgaca gcagcgaaaa ccacgccgcc atcgcgatcg agcgctcgac caggtatccc
    11521 acgccgtaca cggcaacggg gtgggcgaca aaccagagcg cgccgatcag cgcggcgtcg
    11581 tttgccctgc gaagcgggcg ttcgccgccg gccatccgca gcacgattcc gaggaaatga
    11641 tacagggcga tgccgacgca ggcatggatc aggaggttgc ctagccggaa ccacggaatg
    11701 tcgagcccga ccagggcata ggtccagccg aagcttccgt aggagatcca gcgcagggac
    11761 cagcccagca ccggggaggt gcccgcggcc tgcgcgagca ggtccggggt aaaaaatccc
    11821 agatcgtcaa agaccggcgg gttgttgaga aacggcgcgt atattgccgc gctcacgagc
    11881 agcagcagga accacgcgac cacatgaccg gacatggtgc gccagaaaga gacgccaact
    11941 accgttgcgc cgctcgcagc ctgcatgact gggatcactc ggctacggcg ccacggcatg
    12001 atcgctaagc cacccgctcg ggcacgagcg cgcgcgcatc gcgcagcggc tgacgcacca
    12061 acgcagggac gcccggcagt gtcgccgcac cggcttcgat cggcgtgtgc atgcggttat
    12121 attagcgcgc catgcgcatc gccgaccgcc agcgcccgcc gccggggcag ctctacctcg
    12181 atcacgtttc gcatttcgtg cccgatctcg acaaggcggg cgcgctgctg gaagcgctgg
    12241 gctttgccgt cacgccgctc tcggtgcagg tgacgcagca agggcctgcg ggcacgtcga
    12301 accgctgcgt aatgctcgcc gacggctacc tcgagttcct catgccgacg gccgacacgc
    12361 cgaatgcgcg gcgcctgaag aaatccatgc gccgctaccg cggcgtgcac ctcgtgtgct
    12421 tcggcacgcc cgccgccgaa caagaacatc agcgacttgc ggcccacggt ttcgcgccgc
    12481 aaccgatcgt gcacctgaag cgcagggtgg agcgcgggct cagggcgcgc ttttccgtcg
    12541 tgcgcgccgc gcccgggaag atgccggaag ggcgcgtgca gtacgtccag cacctcgcgc
    12601 ccgcggcgat ctggcggccc cagcacctgc ggcacgcgaa cggcgtgctc ggcctcgccg
    12661 ccgcttacat cgtcgccgcg aacccggtgc gcgccgccgc gcgctggtcg cgcttcatgg
    12721 gcggtctgcc gcggcgtgaa ggcaggctcg tctcgctcga caccgaccgc ggcaaggtgc
    12781 tgatcggcac gcgcgcgcag ctcaaagcgc tccttggggc cgcgccgcgc tcgccgggca
    12841 tcgccggcta tgccctcgcc tgccgcgatc cgaaggcgtt tgcgcgccgc tgcgcgcagg
    12901 ccggcctgaa ggtaaaccgc caccttgccg tcgcactccc gcgcgcgctc ggcggcgcgt
    12961 ggcgcctcgt ccccatggag aaacgatgaa gaccgacctc tgcgcccttt ccgccaccga
    13021 gctgctcgcc gcgtatcgcg cgaagacgat ctctcccgtc gacgcgacgc gcgctgcgct
    13081 cgagcgcatc gccaagctca acccggtgct caattgtttc aacctggtgg acgagaaagg
    13141 cgcgctcgcc gcggcacgcg agagcgaggc gcgctggaag aagggtgcgc ccaggggtct
    13201 gctcgacggc gtgccgacct cgatcaaaga tatcctgctg acgaagggct ggccgacgct
    13261 gcgcggctcg aagaccgtcg acccgaaagg tccctggaac gatgacgcgc cggcggtggc
    13321 gcgcctccgg gagcacggcg cggtgctcct cggcaagacg acgacgcccg agttcggctg
    13381 gaaaggcgtc accgacagcc cgctcaccgg cattacgcgc aatccctggg acccgacgaa
    13441 gacgccgggc ggctcgtccg ggggcagcgc cgccgcggtg gcgtccggca tgggcgcgct
    13501 caccgtgggg accgacggcg gcggctcgat ccgcatcccg tgcgcgttca cgggcctgtt
    13561 cggcctcaag ccctcattcg gccgcgtgcc cgcctggccg ctttcgccct tcggcaccgt
    13621 cgcgcacctt gggccgatga cgcgcacggt agcggacgcg gcgctgatgc tgaacgtgct
    13681 cgcgctcccc gatgcgcgcg actggcacgc cctgccctac gacgggcgcg actatcgcgt
    13741 cgggctggag gacggcgtgc gcgggctgcg catcgcctac tcgccggcgc tcggctacgc
    13801 cagggtggac ccggagatcg ccgcgctggt gaagaaggcg gcgcaggcct tcgccgggct
    13861 cggcgcgcac gtcgaggagg tcgatcccgg attcgaggac gcgcagccgc tcttcacgct
    13921 gcactggttc ccgggcgccg cctacgtcgt ggccaacatc cccgaagcca agcgcaagct
    13981 catggacccc gggctgctgg aagtggcgcg cgacggcgcg aagttcagcg tgcgcgagat
    14041 ccaggacgcg gcgctcaagc gcggcgcgct cggcgtgctg atgaaccagt tccacgagaa
    14101 ctacgacctg ctgctcacgc cggcgctgcc gctcgcggcg ttcgaagccg gcatggagat
    14161 ggcggacatc atgaagcagc ggcgctggac cgactggacc ccgttctcgt accccttcaa
    14221 cctcacccag caaccggcgt gctcggtgcc ctgcgggttg acgaaggcgg gactgccggc
    14281 gggattgcag atcgtcggtc cgaagcacgc cgacgcgctc gtgctgcgcg cggcgcgcgc
    14341 ctacgaggcg acgcgcgaat tcagcatgcc acctctgtaa aaaatgggga cagacctcat
    14401 ttttcgcata tcgaaataat gaggtctgtc cccatttaca gccggtagaa gcgcgctgcg
    14461 ttgtcgtgga acagtgcgcg gcgctccgcg ggcgtgaaca cctgcgccac gcgcttgtag
    14521 tcgtcgaaca tgcggcggaa cgtcacgcgc aacctgtcca ccgggaagtt tgaggcgaac
    14581 atgcagcgct cgacgccgaa gacctcgatc gcctcgagca gcacgcgccg gttgctctcg
    14641 tagtcccacg gcgcgtcggg caggcacagg caagacagct tgcagtaaca gtgcgggctc
    14701 gccgcgagcg cgcgcatgcc gcgccgccaa agctcgagcc cgcgcgggct gcggtcccag
    14761 gggaatccgg tgtggttgag caccaccggg atgcccggat gcgcccgcac caccaccgcc
    14821 gcctcctcca gctcccaggt cttcacgcgc aggtcccacg aaagcttgtg gcgctcgagc
    14881 agcgcgtagc ccgcgagcca tgccttctcg cgcatcatgc cgtcgcgaaa cggtttcgag
    14941 cggatgccgc gcacgagggg aaacgacgcc tggcgttcga ggacctccgc ggcgttcggc
    15001 gtgtggaacc aggcgtggcc gacgatggca tgcggcatgc cgtatttcgc attcagagcg
    15061 gtgagccact cagtctcggc gacctgctgc tcgcgcgagc actcggcttc gacatgcacg
    15121 gtcttcacca cctcgtgccc ggcggcgtcg cgccggtagt cctgcggcag atagtcgcgc
    15181 ttcagcttcg cgtagtcgcc gaggaagaac tcgtgcggct cgggatcctg cagccacggg
    15241 taggcgagcg gcccgcggga gaggtcccac aggtggtggt gcgcgtcgac gatcggcagg
    15301 gcgtcgctca gcgtgcgccc gccttctcca ggatggccgc catctccgca taaccggcgc
    15361 gcctggcgag cgcgagcggc gtacggccgt tgcgatcggc gagattcacg ttcgcgcccg
    15421 cttcgaccag cgcttgcagc gtcgccgtgt ggcgcggccc gccgtcgccg agcacgatgg
    15481 attcgatcag cgcggtccag cccaggttgt tgacatgatc gagcggcgcg ccggccttga
    15541 tcagcgtgcg caccacctcg tcgtggccga ggtgcgccgc ggcgatgagc gcggtgccgt
    15601 cgtagcggct ggtggtgttg cccgcacgcg cgccgagctc gagcgacagg cgcagcaccg
    15661 gcagatcgtc ggccaccgcg gcgatggtga cgatgtcgta gcggtcgtac tcgagcgcgt
    15721 tcgcattggc gcccgccttc accagcgcgc gcatggcgcc gagctgcgag cggtaggcgg
    15781 caacgtgcag cggcgtgcgc ttgtagcggt cgcgcgcgtt cgggtctgcg ccgcccgcca
    15841 ccagccggac gatctctgcg gcttcgccgc gcgccgcggc ggcgtgcaag ccggtgtagg
    15901 cggcgagctc ggcagcgccg ggcggcacct gggcgtgggc gcagcctgca aatgcgaggg
    15961 caaccagcag gcgtttcatt gacttactcc ctcgcgcgga cgatgaggct ctgcgtcgag
    16021 cgcccgacca gtccttgctc gtcgtagatg cgcgcctcgg cgaggccatt cgaggacggc
    16081 ccgaaaagcg tacgcgcctc gaggcagatc cactgcccct cgggccgccg cagcaggttg
    16141 atggtcaggt cggagttgac gaacacgtag cgcctgaaat cgaggatcgc gctgatgccg
    16201 ttgccggagt cggcggccac ggcgacccgc tcgatcggca gcggcgcctc gccttcgatc
    16261 agagggtgga gcaggcgaaa ccacacggcg caggggccgc ggaagaacgt gccctcggcg
    16321 acccgccctt ccatgagatc gttatagccg ggcgtgccgg tggcgaatgg aaagcggctg
    16381 ggcggggatt ccgccggcgc cctgggcgcc tgcggcagcg gatgcccggg caggccgtcg
    16441 ggcagcggca ggtcgctctc gcgctgcacg agcgccgtgc agcgcgcgac ttccttgcct
    16501 tccgcggaga gcgaggcgga gaaatgcgcc gcgttgcggc cggcgtagtc cgtctgcacg
    16561 gcaacggcga gctcggcgat cggcaccggc cgcagcaggt tggcggtgac gcgcgcgacg
    16621 tgcgtcaggc cgagcggcgc cgcggcgcgc gcgatcgcgc ccgcgaccag cgcgatcggc
    16681 ggcccgccgt gctggtgctg cgggtcccag gggccgcgcg tgagctcggt cgcgcgccag
    16741 cgcccctcgc cttcccgcac gaaggcgctc gccggcatca acgctcgagc ctcagctcga
    16801 gcacccaggt cgagaccacc ttgtcctggt cgagcatcgc gccgtgcacg gcgtagtgct
    16861 tcgcgtcctt ctgcagcatg cactcgaagc cgcggtagcg attggtggcg ctggcgtaga
    16921 acgagaggat ggcgtcgccg gcgagcacga accgtccgtt gagcgagccg atcgagggat
    16981 tctcgcccga ccagtgcgtg gactgcgcgc ccgccgagaa cggcaccacc tcggtgcggt
    17041 tgcggtgctc ctgcggcgga ttggacttga cgcgcatcac gctctcgctc acccagcgcc
    17101 cggtctcgtg ccgcacgcgc gtctcgccct cgaccgcagt cgcactgccg gcgccgtcgc
    17161 gggtctcgcc cgctgcgcgc cagttgccct cgcgaaacag gaacgagtgc cccatcagcc
    17221 gtgcaccgcc acgctgtcct gcggcgcctg cgcgcgcgcg tccatggtgt agtcgcgcac
    17281 cacgccggcg atgcgcaggc ggtagccggc gaagatcccg gcgcggccct ttttctgcgc
    17341 ctcgcggtgc agctgcacgt tgcgccactt gcgcaccgag gcctcgtcct tccagaactg
    17401 cagcgagacg aacttgccgg gcgaggtgat gctctcgaaa cgctcgagcg agatgaagcc
    17461 ctcggccttt tcgagctcgg gcttgagctg ctcgacgagc tgcatgtact ccgggaagcg
    17521 cccggcggcg ggggtgaact cgaagatgac ggcgatcatg gctcctccgt ggcgagcact
    17581 atacttcccg cccgtggaac cccgccactt cgcgcactgg ccgagcgaca tgccgcgcgg
    17641 gcttgcgctg ccgccgctcc ggctgcacga cctgctcgcg cgcgcggcgt cgcgcttccc
    17701 cgacaagccg gctaccgtgt tccagggcgc gacgctccgc taccgcgagc tcgcggcacg
    17761 cgtcgaggcg ctggccgggg acctgcagca cggctgcggc gtggcgaagg gcgaccgggt
    17821 actcatcgac ctgcagaacg gaccggactt catcaccgcg ctgttcggcg tcctgcgcgc
    17881 ggacgccgtg gcggtgccgg tgagcccggc gaacgtggcc gcggagctgc ggcactacct
    17941 ggaggacagc ggcgcgaagg tcgccatcgc cgagcgcgag gtggcggcgc gattctccgg
    18001 gttgccgctc gagcgcctca tcgtgatgga ggactggcgt ccgggcgaca agccggcggc
    18061 ggcctcgacc cgcgtcgcgg acgatctgtg cctgctgccc tacacctcgg gcagcaccgg
    18121 tcaccccaag ggctgcatgc acacgcacgc cacctgcctg cacaacatcg ccggctcggc
    18181 gctctggaag cacgtcaccg cggacacggt ggcgctcgcc acggcgccct tcttccacgt
    18241 caccggcctg atccacagct tcctcgccac cgtgtacgcc ggcggcacca tcgtcatcca
    18301 gcggcgctgg gatccgctcg cggcggccga gctcgtcgag cgccaccgct gcacgcactg
    18361 ggacaacgtg ccgacgatgg tggtggatct gctctcgcat ccgggcgcgc tcgagcacga
    18421 cgtcggctcg ctcgcctgga tcttcggcgg cggctcggcg atgcccgagg cgatcgcgca
    18481 gaagctgttc gaagtctgcg gcgtgcgcta catcgagggc tacggcatga ccgagaccat
    18541 ctcgcagacg cacatcaacc cgccgcagaa cccgaagaag cagtgcctgg gcatccccac
    18601 cttcgatacc gagtcgctgg tggtggatcc cgagaccttc aaggtgctcg gccccggcga
    18661 gccgggcgag atcgtggtgc gcgggccgca gctgctgaag ggctattggg gaaaggaaat
    18721 ggagttccgc gcgtcctggg ccgaaatcga gggcaggccg tttcttcgca ccggcgacct
    18781 tgggcgcacc gacgaggacg gctacttcta catttcggac cggctgaaac gcatgatcaa
    18841 cgccgccggc tacaaggtct ggcccgcgga agtggaggcc gcgatgtacc agcatccggc
    18901 gatcaaggag tgctgcgtga tcgccgcgcc cgatgcgcgc cgcggcgaga cggtgaaggc
    18961 ggtcgtggtc ctgaagccgg gcatggcggc gagcgccgag gagatcctcg actgggcgcg
    19021 cggccgcatg gccgcctaca aggcgccgcg ccagctcgcg ttcaccgacg cgctgccgcg
    19081 cacgtcctca ggcaagctca tgtggcgcgc cctccaggac gccgaattta aatagggaca
    19141 gacctcattt tcctttttgt ccgcgggacg aataggaaaa tgaggtctgt ccctatttaa
    19201 taagtccaag aaatcacttg gggcgcaggc ccgcgtgcgc gtttcgcaac agcgcgagca
    19261 cgccttcgta agtgaccggc gccggattcg ggtacggcgc ttccatggcg atcttcgccg
    19321 cgcgctcgag gtcggcctcg ggcacgccca ggtcggcgag cttcatctgc agcccgagcc
    19381 tgacttccag gtcgtagagc ccgcccgcgg cgtccgtcgc gccgagcgcg gcggcaatgc
    19441 gcgccatcgc ctccggcgcg gcatcccggt tgtagcgcac ggcgtgcggc aggatgatcg
    19501 aatgggtctc ggcgtgcggc aggttccagg tgccgccgag cgtgtggcag agcttgtggt
    19561 gcagcgcgat gccggtgttg agcgatatcc cggcgagcca ggcgccgtac agcgcttcgc
    19621 tccttgcctc caggtccctg gggttcttca cgatcagcgg cagcgatgcc gccagcgcgc
    19681 ggatgccctc ctccgcgatc agcgacacca ccgggttggc ctccttcgcg tacaaggctt
    19741 ccacgcaatg cgcgatggcg ttcatgccgc tcgccgccgc agtcgcggcc ggcagcccga
    19801 gcgtcagctc gggatcgtag atcacggtct tcggcagcat cttcgggtcg aactgcgtgc
    19861 gcttgcgccc gccttcggtg aagccctgca cccaggtcat ctccgagccc gaataggtcg
    19921 tcggcacggc gagcaccggc atgccggtgg tgagcgcgat ggccttgccc aggccgatgg
    19981 tcgagccccc gcccaccacg atgcagcagt cggcgttcgc cgccttcgcc gcggcgcgcg
    20041 cgtcttccgc cacctcgatc ggcacgtgcc gcaccgcctt gtcgaacagg cccgcgtagc
    20101 gcgcgccgag cgacgcggcg agacgcttca cgtcggccga gcgacccggc gtggacagca
    20161 cgagcgcgcg cttggcgccg agcttgtcca cttcctgcgc caggcgcgcg acgctgccgg
    20221 cgccgaagac gacgcgagag ggcagcgagg tgtaggtgaa cgccttcatg cgcttcgccg
    20281 cagtttagcg agccgccgcc ggaagtcgag cgccagcagc agcagcccga tgcccgcggc
    20341 cgcgaacatc cagcgtgccc cgcccgcatc caccgcgtgc gacgcgagcc acacgcccac
    20401 cgactggccg gtaaacaggc tgcaggcgaa gatcgccagc gcagcgccgc gcgtcgccgg
    20461 cgccatttgc gtcgcgtgca cctgcagcgt gttgtgcatc atgtagatcc ccgcgccggc
    20521 caccaccacg cagggcactg cccagcccgg ccccggcgcc gccgccagtg cgagtaaccc
    20581 gaccgccatc aggccgccgc cgcccagcac caatccccct tcgcccgctc gcggcaacag
    20641 ccgccccgcg ctcgccgcga acagcagccc gcccgccgcg aacgacgccg agaccgcgcc
    20701 gctcgcaccc aggcccaggc cgaagcgctg atgcacgtac aaggcgacga acgcgagcgc
    20761 gccgtagaac aaagcgccct cgatgaacac cgtggcgagg atcacgcgca cccacggcag
    20821 gcgcgtcagg cccgccatgc gccggaacgc ttcggcgagc ggcgtggcat cgatcggctg
    20881 cgcgcgcgtc tgcggattgc cgcgcagctc cagcagcagc agcagcgccg tcgccgcata
    20941 cagaagtgcg aggaggacga acaccgcctg ccagccgtag cgctcgccga gaaatcccga
    21001 cgcggtggtg gcgaaggcga cccccagcat gtgtccgacg agaaagcgcg ccagcaccag
    21061 ctggcgcctg tcgtacgcga ccgcgtcgcc gatccaggcc atcgcgagcg gcacgatgcc
    21121 gcccaccgtc gcgccggtga ggaaacgcgc gacgaccagc gtggcgagcg aaggcgcgag
    21181 cgcgcaggcg aggttgcccg cggccgagat cgcggtgatc agcgccacca tgcggtactt
    21241 gccgaggcga tcggcgagcg ggccgttgac gagctgcagc aggccgtagg ccacggcaaa
    21301 cccggtgatc accgcggcgg cgccgcccgg cgtggtgccg aactccccgg cgatcaacgg
    21361 cagcagcggg tcggcggcgc gcagcgccgc cgcgctggcg aacgccgcga gcgacagcag
    21421 caggacggcg cggccgggca tgcgggcatt atcggacctc aggccacaag ccgcggcagg
    21481 atcgccgcgg cgaaggagag caccaggaag aacccgcaca tgtcggtgac ggtggtgagg
    21541 atcggacccg aagccaccgc cggatcgagc ttcaggcgct tgagcaccag cggcaccgtg
    21601 ccgccgatcg acacggcgac gagcgtgttg gcggcaagcg cgccgcccac caccagcccg
    21661 agccaggggt tgcctttcca caggattgcg gcgagcgcga gcagtgcgcc cagcaccacg
    21721 ccgttgatca gccccacctt cacctcctgc agccacacgc gcagcagctc gaacggcttg
    21781 gtgatgccca gggacagctc gcggatgctc accgccaccg cctggttgcc gctgcagccg
    21841 ctcatgtccg agacgatcgg caggaacacg gccagcgcga tcaccgcggc gagcgtctcc
    21901 tcgtaggccg cgatcacgct cgcggcgatg atgttgagga tgatgttgac cgacagccac
    21961 gacaggcgcc ggcgcgagcg caccatgacc ggcagcgagc ggatttcgtc gccgccgacg
    22021 atgccctgca tcttgcggtg gtcgttctcg gcgcgcgccg cgacctcctc ggcgacgtcg
    22081 ccgcgacgca ccacgccgag cagcgcctgg cgcgcgtcga ccaccggcac cgcggcgacg
    22141 gcttcgcggt cgaaatacgc ctcgagctcg tccagcgttg cgtcgggccg cacgtgcgct
    22201 gcgggcctga gcagctgcgc gagcagggtg tcgcggcgcg cgagcaccag gtcgcgcagg
    22261 tcgagcgcgc cgacgaaccg ccccgccccg gacaccacgt aggcatgcag gtcctcggcg
    22321 aggccctctt cggtgcgccg gttcaggtcc tcgatcacgt cacgcaccgt cgaggtatcg
    22381 gcgtacgaca ggtactcggt caccatcagg ccgccggcga cgtcgcgcgg atacggcagc
    22441 agcttcctcg cttccgcggc ctcttcgggc ggcatgcggg cgaggatcgc ctcggcatcc
    22501 tgcgggtcga ggttggcgag cacgtccacc tgctcgtcgc tctgcatctc gctgacgatg
    22561 gaagccgcgt ccttcacctc gagttcctcg atcaggtcgg cggcgtgctc ctccgggatc
    22621 tcctcgatga ggtcggcggc ttcttccggt gagagcgtgg tgagcacgcg ctcgcgcgcc
    22681 tcgggcgcga ggtgcagcag cgcgcggaac gcctcgctcg gcccgatgga ctcgacgtag
    22741 gcttcgaggg ccctgacgtc gcgggcctgc gcgagcgcct cgagccgctc ccagggcggc
    22801 gtgtcgatga cttcggccat ggctgcactc cctgcgcggc tggcgtgtac gatgctgcga
    22861 ttctatcgac ttgagatcgc acgcatgccc gcgctgcccg atccccacgc ccttgccatc
    22921 atggcgctca cggtgttcgc gctggtgctg ttcgcgcagg atcgcatcgc gctcgagacc
    22981 tccagcctgc tcgtgttcat cacgctggtg gcgggcttcc agatctttcc ctacgcgggc
    23041 gccgacgggc cgctgcgccc gagggagctg ttcgccggct tcggccacga ggcgctggtg
    23101 gcggtgtgcg cgctgatggt gctcggcaag ggcctggaga ccaccggcgc gctgcagccg
    23161 gtggcccgga tcctggcgcg cgtgtggcag accgggcccg cggtgtcgct gctgttcacg
    23221 atgctggtcg ccgcggcgct ttccgcggtg gtgaacaaca cgccgatcgt ggtcatgctg
    23281 ctgccgatcc tggtcggcgt gacccttagc gccaaggcct cgccctcggg cgtgctgctg
    23341 cccttcgggc tcgccacggt gatcggcggc acggtcacca ccatcggcac ctcgaccaac
    23401 ctgctggtgg tgtcggtggc gcgcgacatg ggcgtgcgca acttcggcat gttcgatttc
    23461 gcgctgccga tcgcgctggt gggctttttc ggcatcctgt tcctgtggct ggtggcgccg
    23521 cgcgctgcgg cagcaggcgc gcggcgctga tcagcgacaa gtccgcgcgc ctgttcagcg
    23581 ccatgctgta cgtcgaagac gacagcaaga tcgccggcaa gacgctcgcc gacgcgcgca
    23641 agctggcggg cggcaacctg cgcctgctcg atgtgcagcg cggcgagcgc ggcgtgctgc
    23701 tcgcgcgcct gccctcgctc accttcatgc ccggcgaccg gctgctggtg cgcgacacgc
    23761 ccgagcggct gaaggagctc gagggcatgc tcggcacgcc gctgcacgac caggagaccg
    23821 agtcggccga ggagcgcgag gagaaagaag aaagggcggc cgcggccgag ggcgagccga
    23881 aggcgacggc cgaggaggac gccaagcagc gcctggtcga ggtggtggtg accgaagact
    23941 cgccgctgaa cggctcgacg gtgcggcgct cgcgcttcgc cgacgagtac ggcctgtttg
    24001 tgctcgccgt ccaccgcggc cagttccgcg acgagatccg ccgcggcgag ctgggcgacg
    24061 tggtcctcag caccggcgac gtgctgctgg tgcagggcag cgtgggcgac atcaagcgga
    24121 tgaagtcgaa ccaggcgttc ctggtgctcg attccacggt cgagctgccc gacacggagc
    24181 gcgcggcgct cgcgctcggc atcatgggcg cggtgatcgc cgccgccgcc accggcctgc
    24241 tgccgatcag cgtctcggcg ctggtgggcg tcgccgcgat gatggcgacg cgctgcctga
    24301 cgtggcgcga agcgagcaag ggcctgtccg cgcaggtgat catgatcgtg gtgaccagcc
    24361 tcgcgctcgg caccgcgctc acccgcaccg gcgccacggc ctacgtcgcc gagctgttcg
    24421 tggcggcgac cgccggcatg tcgccgagcg cgatcctcgg cgccctgatg ctgctgatgg
    24481 cggtgctgac caacatcctg tcgaacaacg ccgccggcat catcggcacg ccgatcgcca
    24541 tcggcatcgc cgccaggctc ggcctcgcgc ccgagccgtt cgtgatcgcc atcatcgccg
    24601 gggtgaacct ctcgttcgcc acgccgatgg cctaccagac caacctgctg gtgctgcacg
    24661 ccgggggcta ccgcttcgtc gacttcgtga aggtcggcgt gccgctgtcg ctgctgatgt
    24721 gggcgggcta cctctacgtg atcccgctgt ttttcccgtt ctgagcgcgc gctacaggcg
    24781 cttggccact tcttccagca tcacctcggt ggcgccgccg ccgatcgact gcacgcgcgc
    24841 gtcgcggtac atgcgctcga tcgcggattc cctgatgaag ccgaagccgc cgtggaactg
    24901 caggcagtcg tacaccacct cgttcagcag ctcggcgcag taggccttgg ccatcgacac
    24961 ttccttcacg cagtcgcgcc cgtcggcgtc cagggccgcc gcatgctgca cgagctggcg
    25021 cccggcctcg acgcgcgcgg ccagcatggc gaggcgctgg cggatggcct gcttgtccca
    25081 cagcggcgcg ccgaaagcct ggcgcgcgcg cacgtactcg agcgtcagct cgatcgcctt
    25141 ggccgcctcg cccatgcaca tggccgcaat ggcgatgcgc tcgttctgga agttcttcat
    25201 gatggcgtag aagccgcggt tctcctcgcc gagcaggttg gcggccggga tgcggcaatt
    25261 ctcgaacacg agctccgcgg tgtcggacga gcgccagccc atcttgtcga gcgtgcggcc
    25321 caccgtgaag ccgggcgcgc ccttctcgac catgaacatc gacaccccct ttgcgcccgc
    25381 cgccggctcg gtcttcgccg cgacgaagta cagatccgca agcgcgccat tggtgatgaa
    25441 catcttggcg ccgttcagca cccatgcgcc gccttcgcgc cgcgcgctgg tgcggatcgc
    25501 tttcacgtcc gagcccgcgt cgggctcggt gaccgcgacg gcggtgatcg cctcgccgga
    25561 gacgatcttc ggcaggtacg tcgcgatctg ctccttcgtg cccgcgttcg cgaggtgcgg
    25621 cgaggccatg tcggtatgca ccagcaccgt gatggcgaag ccgccgaagg tggagcgccc
    25681 gagctcttcg gcgagcagcg cggtggcgcg cgcatcgagc gcgccgccac cgtattcctc
    25741 ggcgtagcgc aggccgagga agccgagccg gcccatctgc cgcagcacct cgcgcggcac
    25801 gcagccctgc gcctcccagg tcgccccgtg cgggaccacc tccttctcga cgtagcgccg
    25861 cagcgtgtcg cgcaggagcg cgagggtctg gtcggtcaca gcttgaactc ccggagggcc
    25921 tgctcgcgca ggcggttctt ctgcaccttg ccgctcgcag tcatgccgat cgcttcgaag
    25981 ctgcggacga tgcgcaggta gcgcggcacg cgaaagttcg cgcagcgctc gcggacccag
    26041 gcgaggagcg catcctcggt cagcttgtgt cccgccttca gcgagacgta ggcgcagggg
    26101 acttcgccga gccgcgcgtc gggcacgccg accacctgcg cgctctgcac cgcggggtgc
    26161 ccgagcagca cctcctccac ctccgccggc gcgacgttct cgccgccgac ccggaacacg
    26221 tccttcagcc gcccgagcat gcgcagccgg ccgcccgcgt gcagctcgcc gaggtcgccc
    26281 gtgcgcagcc agccgtcctc ggtgaaggcc tgcgcggttg cttccgggtc gttgtagtag
    26341 ccgcgcatca cgctccagcc gcgcacctgg atctcgccgt ccagcgtgcg cagctgcagc
    26401 tcgggcagcg gcgtggcgag gccctgcacg cgcagcgcct cggcgtcgcg ccagtcgctc
    26461 atgaccacgt tgggcgaggc ttccgacagg ccgtaggcgg cgcagatgcg cttcgcgcca
    26521 agctcgcgga tgatccgctg cagggtctgc ggcccggccg cggcccagcc gccgcgcaag
    26581 acgagcctgc gcctggggaa atcggcgtgc cccatcagca gctggaagat cgtgtcgttg
    26641 ccggaggtga gcgtgcagcg ctccgcttcc atcatggcga gcgccgcacc cgcctcgaag
    26701 gtcggcagcg tcacgaggca tgcgccgtgc acgaggcaca tcagggccga gagcgtcgag
    26761 ccgccgacgt ggaacaacgg acggcagttg aagtagcggt cttcggcctt cacgccgatg
    26821 cgctcgcccg cggcccaggc gttcctcagc atgttgtcgt gcgtgagcat cacgcccttc
    26881 gggtaggcgg tggtgcccga ggtgaactgg atcaggagca agtcgtcggg ccgcacgtcc
    26941 caggcgatct tctgcccgtg atcgagcgcg gcgaacccgc gccacgaatc ctcccccagc
    27001 agcaccttgt gctcgagcag cggcagccgc ggcgcgatct cgcgcagcat cgcggcgtag
    27061 tcgttgccgc ggaacccggg tgccgcgaag atcgcgcggc agtcggcctg cttcaggcag
    27121 aactcgagct cggcggcctt gaagcgcgta ttcaccggca ccgtgaccac gccgaggagc
    27181 gccgcgccgt agaagagcgc aagccagtgc tggtcgttgc ccatcaggat cgcggcgtgg
    27241 tcgcccttgc gcaggcccag gccgtgcatg gcgcgcgcca cgctgtgcgc ggcggcgtgc
    27301 agcgcgccgt agtccagtcg ccgggtcggc gtgacgagcg cctcggcgcg cggcggctgc
    27361 agcgcgagga actcgcccag cacgcgcggc gcccagccgc tcatgccctg cccatccagt
    27421 cgccggcggc gagctgcgcc tcgatcgccg cgagctcggt ctcgagcgcg cccttcgggt
    27481 cgagctcgac gccgcggtcg atggcgcgct tggcgagcgc gagggtccgc gccggggcct
    27541 cgcggatcgt cttcgcgatc tcggcgagcg cggactcgag ccggtccgcg ggcaccaggc
    27601 gcgccaccag gccgtggcgc tcggcttcgt ccgcggagag cgtgcgcccg gtgaacatca
    27661 ggtccttggc gaggcgcttt ccgaggacgc gcggcaggcg ctgcgtcgcg ccgaccgtgc
    27721 cgcgaattgc ctccgggatg cggaacgaag cctgcggcgt ggcgacgatg aagtcgcagg
    27781 ccgccgcgat ctcgcagccc gagcccacgg ccgcgccttc gaccacggcg atcgccggca
    27841 tcggcagcga ttcgagcgcc gcgtaagcag cgaaggcctt catgcgccgc tcgcgcacct
    27901 gcgccgcgga catgccctgg cgctccttca aatcggcgcc ggcgcagaac accggccccc
    27961 tgccccgcac gaagacgagc cgcacggcgt cgtccagccc ctgcgccgca gccatcagcg
    28021 catcgcacat cgcgaggttg agcgcgttgc gggcctcggg ccgattcagc gtgaggatcg
    28081 ccacgccgtc gcgccgctcg acgaggaccg tcatatcgct ttcgccttgc gcagctcgtc
    28141 gatgcgcgcg tcgctgtagc cgagacgctc cttgaggatc gcctcggtgt gctcgccgag
    28201 cgccggcggg ggtccgatct gcggatcgtc ccagccgagg aacttgtagg gcagcgccag
    28261 cgccttgaat tcgcccacgc ccggatagtc gaagctgccg acgagctggc gcgcgcgcac
    28321 gtgcggatcg gcgagcacct cggcgacgtc gttcaccggc cccaccggca cgtcggcggc
    28381 gtcgagactg gcgatcagct ccgcgcgcgt cagcttcgcg accgcggcgc ccatcgccgc
    28441 catcacctcg tcgcggcgcg cgacgcgcgc ggcgttggtg ccgtagatgg cgaccgtccc
    28501 catttgcagc gcctcgcaga gcttgggcca gtgctggtcg ctggcggtga tgtgggcgta
    28561 cttgccgtcc ttgcagcgaa acgtcgccga gggcacgcgc cccgggtgct cggtgccgag
    28621 gcgcggcggg acttcaccga gcgcgaagta gcgcgcggcg gcgagcgtca gcaggctcac
    28681 ctgcccatcg agcatcgaga aatcgacgta tgcgccacgg ccggtccgct cgcgcccggc
    28741 gagcgcggcg agcatcgcga tcgccaccca caggcccgag gtgaggtcgg ccagcggcag
    28801 tcccggcttc acggggccgc cgcccttctc ccccgtgagg ctcatcagcc cgcccatcgc
    28861 ctggaacacg gtgtcgtaac ccttcttctg cgcgtagggc ccgctctgcc cgaagcccgt
    28921 gcaggagacg tagacgagct tcgggttgag cgaggacagc gccgggtagt cgagtccctg
    28981 gcgtgcgagc gtgccgaccg ggaagttctc caccagggcg tcggcctgcc gcgcgagctc
    29041 gcggatgatg cggcgcccgc cgtcgcttct caggttgacc gtgatcgagg ccttggcgcg
    29101 gttgcaggcg aagtagtagg cgctgtcctt tgcgagctgc ggctcgaagc cgcgcgtctc
    29161 gtcgcccgcg ccgggttgct ccaccttgat caccgtggcg ccgagctcgg cgaggatcat
    29221 gctcgcgaac gggcacgcca gcacgcgcga gaggtccagg atcgtcaggt gctcgagcgg
    29281 cttcatctca cctgaacgcc agggctgcga tgccgacgag caccagcgcc gcgccgctca
    29341 cggtgcgccc ggtgagctcc tcgcggttct tgagtatcca gacgctgacc gggaagatga
    29401 cgagcggcgt cgtgtgcacc accagcaccg cgagcgcgat ctcgatctgc gtcatggcga
    29461 gcgcgatgaa gatcgaaccg agcgaggtga gcacgccgca cccggtgtag agcaggagcg
    29521 ccgtgcggtc cgcggcgcgc agttgcgctg cgactctgcc ccactggtgc gtcaccacga
    29581 cctggcaggc cagtgccgcg accgacgcga tcaccgtgcc gagcgctgcc tcgctccact
    29641 cgcgcatcgc gaggccgcgc agcacgttgc cgaagccgaa gcccgccacc gtcagcagcc
    29701 cggcgaggaa gcccttgcgc aacacctccg gcgcgagcgc gtgcagcgag ccggtgcgcg
    29761 gctgcgtcag ccacagcccg atccacacca gcaccatgag cgccagctca tacgggccga
    29821 gcagctcgcc cgcgagcagc cacgccgcga ccagcgcgaa ggccggcgcg gtcgaatgga
    29881 acacactcgc gcgcgagggc ccgagcagct tcaccgtgtc gaagagaaag cgccggccga
    29941 ggaacgtgcc gatcaccccg ccgatggcga acaggcccgc gcctttccag ctccagccgt
    30001 agggcgccgc acgcaccccc agctccacgg cgaagagcag cgccggaaac acgatgttgg
    30061 tcgccagcac gatgaagaaa cccgcctcca ccggcatcct cgccacagcg aagcgcgtga
    30121 cgacgatgtt ggtggcgaag gcgagcatcg ccaggagcgc gtacgtgatg cccatggctc
    30181 atggcttgcg aaaggcgcgc atcctcttgt tggcttcgcg cccgcgcgcg agggcttgct
    30241 cgagcggcag gtcggcgact tcatggaaca gctgcttggt gagccgcagc gccgggcggc
    30301 cgagggcggc gagcttgtcc gccagcgcca cggcctcttt cagcacctgg gcgcgcggca
    30361 ccacgcggtt gacgaggccg agcgcgaggg cgcgcgccgc gcccaccggc tcgccgagcg
    30421 cgaccagctc gaaggccgcc ttgcggccga cctgccgcac caggttcgcc atcacgatgg
    30481 cggcgacgat gccgtggcgc acctcggggt agccgagcgc ggcgccgtcg cccatgacca
    30541 ccaggtcgcc cgcgatggcg agccccgcgc cgccgccgag cgccgcgccg tgcaccgcgc
    30601 tcaccacggg cttcgccagc ttcgggatgg cgaggtgcag ctgcatggtg agctcggcgc
    30661 gtgcctcgac cttcgccttg tgctcgggcg tgagcccggc gaactcggtc acgtcggcgc
    30721 cggcgcagaa cgccgggccc gcgcccgcga gcaccacgca gcccacgctc tccgcggcgt
    30781 ccgcagtgcg cagcgcatcg agcagcgcct gcgtcagctc ggtattcagc gcgttgcgct
    30841 tgtccgggcg gttcagggtg agcacccgca ccgcgccctg gtccttgacg agaagcacgc
    30901 tcatagaaaa atgaggtctg tccccattta cagtgccgcg aggcgcgcgt tggaccaggc
    30961 ccagctcatc gccgcctgca acaccgggtc gcggatgccg aggagctcgg tgatgcgcgt
    31021 cttcacgcct tctcccgctc gcgcgcgatc agctcgcgcg cgcgcgccgc gagtttctcg
    31081 agcgccgcgt cgaacgccgc gcgctccctt gcgccgaggc agtcgaggaa cgcgtcgttc
    31141 ctctccgcgg cggcgcggat cagcccctgg tacagcgcgc gcccggcgcg cgtcagcgtg
    31201 aggcgcacgc cgcgcgcgtc ctcggcgtcg atcgagcgca gcacgaggcg gcgcgcggcg
    31261 aggccggaga ccacgcggct catctggctc ttgtcgatgc ccgcggcgct cgccagatcg
    31321 ttcagcgact gcggctcggc aacgccgccg aggagcgcga tcgtgcgcca ctcccacagg
    31381 ctcacgccga actcgcgccg gtagcgcagc tcggcgccgc gcgacagcag gtttgcgacc
    31441 acgtgcagcc ggtaggaaag cagcgccccg atgccgggcg cgctagtcgt cgaagaacgg
    31501 ggtttcatgc tcgccgcgca gtttcaggtc gaagcggtag cgcccgggcg cctcctcgcg
    31561 cgcgacgagc agcgcccggc gcgccgccgg caccgccgcc agcgccgggt cggcatcgtt
    31621 cgccggatcg ccgggaaaga acatcgtcgt cctgaggatg cgcatcaggc ccgagaacat
    31681 caccatcacg ctgaggtgcg gcgcgcggcc ctcgggcgcg ccaggacgga tggtgcggaa
    31741 cgcgtagtgg cccgccttgt cggtcgccgc gcggccccag ccgaggaagt ccgggtcggc
    31801 ctcggcatgg cgcggatcgg cagggtggcg gtagatgccc tgcgcgtccg cctgccagat
    31861 ctccacgatc aggttgtcga gcggcgtgcc gtcctcctgg cgcacgcagc cgacgatctc
    31921 gatggcctcg ccccgcgcct ttcggcccgc gagctgcgtg aggtcgctcg cgcccgcgtc
    31981 gacatagcgc ggcgggaaga acgggccgag ggtggcttcg ccggtgggga tgttcatggc
    32041 tgcgagggcg tcgcccgcct cccgcgcagc acgatccgat gctcgtacac gagcgcgttg
    32101 cggggctcgc cggtcggcgg caggagcctg gccaccagcc gcccgcgcgc ggcgacgtcg
    32161 ggcaccgcgt tgaagatccg gtcctgcgcg ttcagcggct cgcccgggaa gtacatctgc
    32221 gtcacgaggc gctgcagcca cactttgccg aagaccgaga agtggatgtg cggcggccgc
    32281 caccagccgt cgttggtcgg caccgggtag gcgccgggct tcacggtgcg cagctcgagc
    32341 aggccgccgg cgtcggtggc caggcgcgcg gcgccgtaga agtttacgtc caccggcgcg
    32401 tggtcgtcgt cgttcgggtg gatgtagcgc ccggcggcgt tcgcctgcca gacctcgacc
    32461 acgctgccgc cgaccggcgc gccgtcctcg tcgacgacgc tcagcctgag ctgcatcagc
    32521 tgcccgatcg cctgcttttc cccgcggccg gcgatgtcgc cccccaggag ctgcacacgc
    32581 ctggcgacct gcagcggccc ggtgagctcg gagagcgtcg gcgcgcgccg caccagcggc
    32641 tgcgtcgggt tgcgccacgg cgtctgcgga tagccgggca cgatgcgcgt gggctgggtg
    32701 cgctcgtcgc gcggcgtgat cacgacctgc atcgttgaca gcctagcgcc agttagttga
    32761 tgatgtcaac gatggccgcg gtgacgctct gcgagtgctt cgcgcgcgac gggctgcagc
    32821 acgagcccgc cttcgtgccc acggcggtga agcgcgcgct gatcgagcgc ttcgccacgc
    32881 tcggcttccc gcgcatcgag gccacctcgt acgccaatcc gaaggtcgtg ccgcagttcg
    32941 ccgacgcgag cgagctgctg caggccctgc cccggcgccc cggcgccttc tacaaggcga
    33001 cctgcgccaa cccgaaggcg gtcgagcgcg cgctcgccga cctcggcgcc gggttcggcg
    33061 ccaacgaggt gagcctgctg gtctcggcga gcgaggcgca cacgcagaag aacctcaacc
    33121 gcacgcgcgc cgagcaatgg gcgaacgtgg aggcgatggt gcgcctcgcg ggcgggcgct
    33181 tccggctggt gggcacggtt tcggtcgcct tcggctgccc gttcgaaggc aaagtcgacc
    33241 cgggcgtggt ggccgaggac gtggcgcgtt tcgtcgcgct cggcgtcacg cacgtcgcgc
    33301 tcggcgacac tacggggctc gccacgccga aggcggtgaa ggccatggtc gcgcgcctgc
    33361 ccgcgggcgc aacgccgatc gcgcacttcc acgacacccg cggcacgggg ctcgtcaact
    33421 atgtggctgc cttcgaagcc ggcattcgct tgttcgattc ctccttcggc ggcgcgggcg
    33481 ggcatccggc gaagctgcag tacggcgagg gccataccgg caacgtgtgc accgaggacc
    33541 tcgtcaccct gttcgagtcg atggacgtgg ccaccggcat cgacctcgac gggctgctcg
    33601 agacggcgcg tttctgcgaa gcggcgctcg gacgcgagct gcacgggcgc gtcacgcgcc
    33661 tcgtgggtta aactcggccg cgacctcccc gatgaagctc ggatacttca ccatgccgct
    33721 gcacccgccg ggacgcccgt gggtggagac gctgcgcgag gaccgcgagg cgatcctgct
    33781 gtgcgaccgc ctcggctacg ccgaggcctt cgtcggcgag cacgcgaccg acgccgccga
    33841 gtcgatcacc tcctgcgcca cgttcctcgc ttcgctggtg cacgacacca agcgcatcaa
    33901 gctcggcacc ggcacgctga acctctccaa ctcgcacccg gtgacggtcg ccaccacggt
    33961 ggcgatgctc gataccctgc tcgagggccg cttcatcatg ggcatcagcc cgggcgggct
    34021 gccttcggac atggaagtgt tcgagaacct gggcaaggac cgcggcaaga tcttcctcga
    34081 gtgcatcaac cacgtgctcg ccatctgggc gggcgagccg ccgtacgaca tcaagggcga
    34141 gatctggaac gtctccacgg cgaagacgca gatcctcgac atcggccagg gcgtgatgct
    34201 caagccctac cagaagccgc acccgccgat cgtggtgacc gcggtcgagc cgttctcgaa
    34261 gggcgtgacg gcggccgcgg cgcgcggctg ggatccgatc tccgccaact tcctgctgcc
    34321 gcagtgggtg aagtcgcact gggggcgcta cgccgagggc tgcaaggagg gcgggcgcgc
    34381 cgccgacccg gccaactggc gcgtggcgaa gacggtgttc gtcgccgacg acgccgccaa
    34441 ggcgaaggcc tacggccgcg gggcgcagag cccctaccgc ttctattact cgcagctcct
    34501 caccaagctc aagcgcgccg gccgcgccaa cctgttcaag cacgccaagg acgcaccgga
    34561 cgactcggtg acgctcgacg gcgtaatgaa cgacctggtg atcgccggaa cggtgaacga
    34621 ggtggtagac cggctgctcg ccttccacgc gcaggcgggc gatttcggca cgctgcttta
    34681 ttgcggcatc gactgggccg acgcggcgct cgcgcgccgc tcgctcgagc tgatggcgga
    34741 agaagtgatg ccggcggtca acaacgccat cggcggaaag gagacaaagc gtgcgtctgc
    34801 tctctagccg cctgccctac tcggcgatcg tcgaccggcc gccgctgaag ctgccgcagg
    34861 gcgggcggct catcgtctgg accatcgtca acgtcgagga gtggtcgatc gagcggccga
    34921 tgccgcgcac ggtgctctcg ccgccgctcg gccagccgct gctgcccgac ctggccaact
    34981 gggcctggca cgagtacggc atgcgcgtgg ggttctggcg gctgctggaa gcgctgcagc
    35041 gcctcaagat cacgccgacg ctggcgatca acggctcggt gtgcaagacc tatccgcgcg
    35101 tcgcgcaggc cgcgcacgag gcgctctggg agttcatggg ccacggctac gtgcagcggc
    35161 cgatgcacca cctcgagaac cagcggcagg cgatcgccga cacggtcgcc gcgatccgcg
    35221 agtgcaccgg gaaggcgccg cgcggctggg agagccccgg gctctcggag acgccggaga
    35281 cgctcgacct gctggccgag gccgggttcg agtacgtcgc caactggccg gccgacgacc
    35341 agccctgccg cgtcgagacc gccaagggca cgatggtggc gctgccctac acgctggaga
    35401 tgaacgacat cccgatgatg gtggcgcagg gccacccttc ggaggagtgg ctgcgccgcg
    35461 gcatggagca gttcgaccgc ctgtacgccg agagcgagcg catcacccgg gtgatggcgg
    35521 tgagcgtgca cccgtacatc accggggtgg cgcaccgcat cgggtatttc gagaagctgt
    35581 accagcacat ggcgcgccgg cccggcgtgc tgcactggac cggcgaaagg atctgcgact
    35641 ggtatgtcag cgaaacccaa aaaggcaact gaagcccgcg ctgcggacta cgacgagctc
    35701 tggggccgcg ccgagcagct cctgcccgtg ctgcgcgagc gcgccgccgg ctgcga
//
LOCUS       DBNHKE010000130        14330 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_973638, whole genome shotgun sequence.
ACCESSION   DBNHKE010000130 DBNHKE010000000
VERSION     DBNHKE010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_973638"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="AC16O9_08595"
     CDS             complement(<1..784)
                     /locus_tag="AC16O9_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6516712.1"
                     /translation="MKRAAYVLLGLPLLFAMAALAWLVASEPGLRWAMARAEAAAGGK
                     LAIEEARGTFTTTISLGRVSYADGGVEVEAREVQAEADALALLRGRIGVEPLRIEVLR
                     VTLKRGTKAPATGSGMDPPWHLRVARASISRFELRLDDERHLLTDLRFTHAAVGPNLA
                     AAAGSFARPDARFPAEVRFDVTGTPGRLSAKLSATIAGVPATADLVVVPAASPALRSL
                     EARAGPLDPSRFEAALPKALLSARLEAKPAGAGYAGALAGANG"
     gene            complement(784..2523)
                     /locus_tag="AC16O9_08600"
     CDS             complement(784..2523)
                     /locus_tag="AC16O9_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HIR6516713.1"
                     /translation="MHGIVALFVLSAALAGLPVAAAGLDYRVEVDAPDALATPLRKGL
                     NLIRWQGDPQMTPGLLRRLANEAGEDARGVAAAEGYFSPRVSVTIDETVQPWRVRLLL
                     DPGARTTVAGTEIRFSGPAADDPQAADLLAGVRRSWSLRRGQPFRQEDWDAAKRGAVA
                     ELSRWRYAAARVAASEARIDPERREAQLEVELDSGPPFRFGPVQVAGTERYAPALVEN
                     LSPVRPGDDYDRATLVVYERRLNETGYFASVRTEIDAQAGNAASAPLRVAVIEAPSKQ
                     VESGVSYNTDVGPRFQVRYSDHDLRDSAWRLRSALALDAKIQELKLDLDAPPRPGGRW
                     NHYFARVAQTDIQNELARSFALGIAHNFGTDVAPSAAILSWHLEDQRAGSVVTDSRHA
                     VYAGLRRTFRRTDEIVAPRSGYFGSVELGGGLPGVSTEKFLRAVGRASLLFALGRNGD
                     LLLRAQGGFVNSGTRLGIPTDFLFRTGGDQTVRGYAFESIGVRQGDAIVGGRRLVVAS
                     AEYTHWVGENWGVAAFVDAGDAWDKDEPFDAALGYGLGARFRTPIGPARADLAYGRDR
                     REYQLHISIGFAF"
     gene            2623..4581
                     /locus_tag="AC16O9_08605"
     CDS             2623..4581
                     /locus_tag="AC16O9_08605"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6516714.1"
                     /translation="MTVDIGTLDTFPKLLLHHAQVRGDLPAIREKDLGIWQTWTWRRF
                     ADEVRTLACALADQGLKRGDHMALVGDNRPRIYAAMCAAQCLGAIPVPLYQDAVAAEM
                     AFPIQNAEIEHALAEDQEQVDKLLEILPQCPTLKRIIYDEPRGMRSYAQRELTSYDAL
                     LESGRAALKRDPAFLEAEIAKGRGEDTAAMFFTSGTTGTPKGVVLTHHALIDRAKAAA
                     ELEGLGDRDVVLAYLPPAWIGQNIFSYAQPFVTGYCICCPESAETVMTDMREIGPTYY
                     FAPPRVLEALLTQVSIRMEDAGAVKRWLYRRFMDVARRVGGMLLDGKPVGIADRLRYG
                     IGDLLVYGPLRNVLGMSRVRVAYTAGEAIGPDLFKFYRSIGINLKQLYGSTETSVFVC
                     VQPNGQVKSDTVGPAVPGVELKFTPERELLIRSPGLFKEYYKNPQATMQAKDREGWFH
                     TGDAGYVDPDGHLKIIDRVKDVGKLADGTLFAPKYLENKLKFFPYIQEAVTFGHQRDR
                     VCAFINIDAEAVGNWAEKRNMPYSGYVDLASRDEVYELVRECVERVNADLAQEPEVAN
                     SQIHRFLILHKVLDADDEELTRTRKVRRGFVAQKYATLVDALYGGKDAVHVEAQVKYE
                     DGRTGTISADLKIRDAKTFRPEAAKRAA"
     gene            4578..5378
                     /locus_tag="AC16O9_08610"
     CDS             4578..5378
                     /locus_tag="AC16O9_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516715.1"
                     /translation="MSRAEHIGETVLSVEKVSLAFGGVKALQEVSFDVREHEVRAIIG
                     PNGAGKSSMLNVINGVYHPQQGTIRLRANGAWKEFHDMDSHEAASMGIARTFQNIALF
                     KGMTVLDNVMTGRNLKMRSSFIEQAIWIGRARREELAHRHAVEEIIDFMEIQHIRKTP
                     VGKLPYGLQKRVELARALAAEPRLLLLDEPMAGMNLEEKQDMCRFVLDVNDHYGTTIV
                     LIEHDMGVVMDISDRVVVLDYGRKIGDGTPDEIRGSQTVIDAYLGVAH"
     gene            5375..6277
                     /locus_tag="AC16O9_08615"
     CDS             5375..6277
                     /locus_tag="AC16O9_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516716.1"
                     /translation="MTNVSFFFEVLIGGLLAGVMYSLVALGFVLIYKASGVFNFAQGA
                     MVFLAALTFVSVVKMGVNFWLALVITLAVMVVLGIGIEKIVLRPLVNQPPITLFMATI
                     GLTFVLEGLSQMVWGSQPHALELGIKDVPMQWLADATGMFISQFDLFASGIAAVLVAS
                     LALFFQFTRVGRALRAVADDHQAALAVGIPLKQIWAIVWAVAGFVALVAGMMWGARNG
                     VQYALTFIALKALPVLILGGFESIPGAIIGGLIIGASEKLAEVYIGPMVGGGIESWFA
                     YVIALLFLLVRPQGLFGEKIIDRV"
     gene            6280..7356
                     /locus_tag="AC16O9_08620"
     CDS             6280..7356
                     /locus_tag="AC16O9_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516717.1"
                     /translation="MLYREAGQFKSTYAEDQQIFPIRQDRIAVALLLLVAFVAVPVFA
                     NEYWLSAILTPFLIFALAALGLNILTGYAGQLSLGTAAFMAVGAFMAYNFVLRLPWLG
                     IIPSFVLAGLCAAAVGIVFGLPSLRIKGFYLAVATLACQFFVLWAIQRIGWFTNNSPS
                     GVITAQTVEILGYAFDTPESKYLLTLGIVAVLALLAKNLMRSETGRAFMAVRDMDVAA
                     SVIGIPMMQTKLLAFAISSFYCGVAGALYAYTYLGTVEPEAFTLDLSFRILFMIIIGG
                     VGSILGSFLGAAFIILLPIFLSVLFGVASHTFGLDISHAIVSNLELMIFGGLIIFFLI
                     VEPHGLARLWQIAKEKLRLWPFPH"
     gene            7451..8773
                     /locus_tag="AC16O9_08625"
     CDS             7451..8773
                     /locus_tag="AC16O9_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516718.1"
                     /translation="MKRTTFALALALAAGFSGAVLAQAKEQFVPANFYWVGPYAPGGS
                     GFGGGIIDYMQMLNERDGGINGVKLTWEKCETEYNNARGVECYERSKNKGPTGATVIH
                     PLSTGITYSLIEKATTDRIPIISMGYGRASAADGRVFPYVFPLITTYWSQASSMVNFL
                     GNRAGGMDKLKGKKIVLLYHDSAYGKEPIPILTDLSKDFGYQLTTIAVPHPGNEQQAQ
                     WLQIRQLQPDWVILWGWGVMNPTAIKTAARTGYPREKMVGVWWSGAEEDVIPAGSAAK
                     GYISAGFNLPGSNFPVMRDIQKYVYAKGKGEFDDKSRFGSIYHTRGVVAGIVTTEAIR
                     AAQAKFGKGKPITGEQMQWGIENLKIDEKRLKELGAVGLMPPIEVSCFNHEGSGLVKY
                     QQWDGSKWIPITDWVEPDYKLVRAKVEADAAQYAKEKGITPRDCPKGG"
     gene            8826..9626
                     /locus_tag="AC16O9_08630"
     CDS             8826..9626
                     /locus_tag="AC16O9_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516719.1"
                     /translation="MATAAFLSVSNVEVIYDHVILVLRGVSLQVQEGQIVTLLGANGA
                     GKTTTLKAISNLLRAERGDVTKGYIEFQGQRIDKLNPSELVKRGICQVMEGRHCFQHL
                     TVEENLLTGAYTRGNADLSEDLEKVYRYFPRLKERRKAQSGYTSGGEQQMTAIGRALM
                     ARPKMILLDEPSMGLAPQLVEEIFEIVKSLNQKENVSFLLAEQNTNIALRYADYGYIL
                     ENGRVVMDGEGRSLAENEDVKEFYLGFSTGGRKSFRDVKSYRRRKRWL"
     gene            9623..10873
                     /locus_tag="AC16O9_08635"
     CDS             9623..10873
                     /locus_tag="AC16O9_08635"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR6516720.1"
                     /translation="MSPFYDALETRPAEAREAALMAALPKHLAHAKANAPGWARILKD
                     VDAASVRSREALAKLPVTRKSELAELQKAHPPFGGLNATPVDKLARVFASPGPVYDPE
                     GGGADWWRTARSLYAAGFRPGELVLNGFSYHFTPAASMVEHGARAIGCTVVPGGTGQT
                     EMQAATIRDLRVTAFGGTPSFLKLIIEKADELKVDVSSLSKAIVGAEYLPPALRQAMG
                     ARGIRAMQLYASADLGLIAYESAMPDGAINEGMVLDEGLILEIVRPGTGDPVPAGEVG
                     EVVVTSFNKDYPLIRFATGDLSAVLAGKSPCGRTNTRIKGWMGRADQTTKVRAMFVTP
                     KQVGEVLGRHKEVLKARLVVAGETGNDSMTLKCEVRERAAGLAEAIVASIRDVTKLRG
                     EVELVSPGTLPNDGKVIDDTRKYT"
     gene            complement(10875..11519)
                     /locus_tag="AC16O9_08640"
     CDS             complement(10875..11519)
                     /locus_tag="AC16O9_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516721.1"
                     /translation="MFVLAGCAMMPGQSGEMSFFITSAGPGNGADLGGLEGADKHCQT
                     LAAAAGAGDRTWRAYLSTQGNALNDPKVVHARDRIGSGPWHNAKGVRVARDVKDLHSA
                     RANVNKETALDEKGQMVNGRTEKPNKHDILTGSRPDGTAHPPTAGGRFPDQTCGNWTK
                     SGDGSAMTGHHDRAGPIDHAWATSWNSAHPTIGCSQKALQATGGDGLFYCFAVK"
     gene            complement(11715..12341)
                     /locus_tag="AC16O9_08645"
     CDS             complement(11715..12341)
                     /locus_tag="AC16O9_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6516722.1"
                     /translation="MRPVTLYFDFISPFSYIAVKRLHELPRDVTLELTPVLFAGILNH
                     IGQKGPAEIPAKRLWSYRWCTWWAQSLGVPFRFPASHPFNPLHHLRLAIAAGVSRKNV
                     ERIFEAIWTTGAEAADPAAFMTLAKDLGVDEAKLASPEVKETLRRNTEGAIARGVFGV
                     PTYEADGELFWGADSLGFLNAFLADPAVLRNAEMRRVDELPVGAARKP"
     gene            complement(12329..12502)
                     /locus_tag="AC16O9_08650"
     CDS             complement(12329..12502)
                     /locus_tag="AC16O9_08650"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR6516723.1"
                     /translation="MKSPCNKICVMDAQNRFCRGCQRTLDEIARWGAMSDAEREAVLA
                     ELPVRKEDPACVR"
     gene            complement(12499..13410)
                     /locus_tag="AC16O9_08655"
     CDS             complement(12499..13410)
                     /locus_tag="AC16O9_08655"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6516724.1"
                     /translation="MAAMPKKVRIVEVGPRDGLQNEKMEVPTKVKLELIARLADAGLP
                     AVEATAFVSPKWIPQMADHTEVLEGIQRRAGVSYPVLTPNLKGFEAARAAGATEVAVF
                     GAASESFSKKNINCSIAESLERFRPVAEAARKANIKVRGYISCVLGCPYEGEIAPAKV
                     AEVARALDAMGCSEISLGDTIGTGTPGKTKAMIEACARAVPLERLAGHYHDTYGQALA
                     NIYASLELGVATFDSSVAGLGGCPYAKGASGNVATEDVVYMLNGLGIDTGVDLAKLVA
                     TGQWICGVIGKEPQSKAARAIAAKQAA"
     gene            complement(13413..>14330)
                     /locus_tag="AC16O9_08660"
     CDS             complement(13413..>14330)
                     /locus_tag="AC16O9_08660"
                     /inference="COORDINATES: protein motif:HMM:NF044986.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR6516725.1"
                     /translation="LRFPEATPGVRVDTGVEPGAAITPYYDPMIAKLIAWGEDRPAAL
                     ARLGAALAAVEIAGVQTNVVFLERVVRSRAFATADLDTGLIERSRAELFPQAQPASHE
                     LLAAAAMAELLEEADEARARAAGSGDPYSPWDSVDGWRLNQGSHHHFVFAEPGARHEV
                     TLQFRPGAYGLTIDGREYALAGERTAPGSLRLQLDGRVFEARAVHVAHDWHVSAGGAR
                     AVLRLEDGLPADEDESAPGSLAAPMPGKVIALLVKPGAKVEKGAPLLILEAMKMEHTI
                     SAPADGVVKAIHFAAGEQVPEGAELLTLE"
BASE COUNT         2280 a         4818 c         4903 g         2329 t
ORIGIN      
        1 cgccattggc gccggcgagc gcaccggcgt agccggcgcc cgccggcttc gcctcgagcc
       61 tcgcgctgag caaggctttg ggcagcgccg cctcgaagcg cgacgggtcc agcggtcccg
      121 cgcgcgcctc gagcgagcgc agagcgggcg acgcggcggg caccaccacc aggtcggccg
      181 tcgcgggaac gccggcgatg gttgccgaaa gcttcgcgct cagccgcccg ggcgtgccag
      241 tcacgtcgaa gcgcacctcg gcgggaaagc gcgcgtccgg ccgcgcgaat gacccggccg
      301 ccgcggcgag gttcgggccg actgcggcat gcgtgaatcg cagatcggtg agcaggtgcc
      361 gctcgtcgtc gaggcgcagc tcgaagcgcg agatcgatgc gcgcgcgacg cgcaggtgcc
      421 agggcggatc catgccggat ccggtcgccg gcgccttggt gccgcgcttc agcgtgacgc
      481 gcaggacctc gatgcgcagc ggctcgacgc cgattctccc gcgcagcagc gccagcgcat
      541 cggcctcggc ctgcacctcg cgcgcctcga cctccacgcc gccgtcggcg tacgacacgc
      601 gcccgaggct tatagtcgtg gtgaacgtac cgcgagcttc ctcgatcgca agcttgccgc
      661 cggccgccgc ctcggcgcgc gccatcgccc agcgcaggcc cggctcgctt gccacgagcc
      721 aggcgagcgc cgccatcgca aagagcagtg gcaggccaag gagcacgtag gcggcgcgct
      781 tcatcagaac gcgaacccga tcgagatgtg cagctgatac tcgcgcctgt cgcggccgta
      841 agcgaggtcg gcacgcgccg ggccaatagg cgtacggaac cgcgcgccca ggccgtagcc
      901 gagggccgcg tcgaagggct cgtccttgtc ccacgcgtcg cccgcgtcga cgaacgccgc
      961 gacaccccag ttctcgccca cccagtgcgt gtattcggcg ctcgccacga caaggcgccg
     1021 gccgccgacg atggcgtcgc cctggcgcac gccgatgctc tcgaacgcat agccgcgcac
     1081 cgtctggtcg ccgccggtgc gaaaaaggaa atccgtcggg atgccaaggc gcgtgcccga
     1141 gttcacaaac ccgccctggg cacgcagcag caggtcgccg ttgcggccca atgcaaagag
     1201 caggctcgcc cggccgacgg cacgcaggaa cttctcggtc gagacgcccg gcaggccgcc
     1261 gcccagctcg acgctgccga agtagcccga gcgcggcgcg acgatctcgt cggtgcggcg
     1321 gaaggtgcgc cgcaggcccg cgtagaccgc gtggcggctg tccgtgacga cgctgccggc
     1381 gcgctggtcc tcgaggtgcc aggacaggat cgccgccgag ggcgccacgt cggtgccgaa
     1441 attgtgcgca atgccgagcg cgaacgagcg cgcgagctcg ttctggatgt cggtctgcgc
     1501 cacccgcgca aaatagtggt tccagcgccc gcccggacgc ggcggcgcat cgaggtcgag
     1561 cttgagctcc tggatcttcg cgtcgagcgc aagcgcgctg cgcagccgcc aggccgaatc
     1621 gcgcaggtcg tggtcgctgt agcgcacctg gaagcgcggg ccgacatcgg tgttgtagct
     1681 gacgccgctt tccacctgct tggagggcgc ctcgatgacc gcgacgcgca gcggcgcgct
     1741 cgctgcattt ccggcctgcg cgtcgatctc ggtgcgcacg cttgcgaaat agccggtttc
     1801 gttgaggcgg cgctcgtaca cgacgagcgt ggcacggtcg tagtcgtccc cggggcgcac
     1861 cgggctcagg ttctcgacca gcgcgggcgc atagcgctcc gttcccgcca cctgcaccgg
     1921 gccgaagcga aacggcgggc cgctgtcgag ttcgacctcc agctgcgcct cgcgccgctc
     1981 ggggtcaatg cgcgcctcgc tcgcggccac gcgcgcagcc gcatagcgcc agcgcgacag
     2041 ctctgcgacc gccccgcgct tggccgcgtc ccagtcctcc tggcggaacg gctggccgcg
     2101 gcgcaggctc caggaccggc gcacgccggc gagcaggtcc gccgcctgcg ggtcgtcggc
     2161 cgcgggcccg ctgaagcgga tctcggtccc ggcgaccgtg gtccgcgcgc cgggatccag
     2221 cagcaggcgt acccgccagg gctggactgt ttcgtcgatc gtcacgctca cccggggcga
     2281 gaaatagccc tcggcggcgg ccacgccgcg tgcatcctcg cccgcctcgt tggccagccg
     2341 ccgcagcagc cctggggtca tctgtgggtc accctgccag cggatgaggt tcaggccctt
     2401 gcgcagcggc gtggcaagcg catccggtgc gtcgacttcg acgcggtaat cgaggccggc
     2461 ggcggctacc gggaggccgg ccagggccgc ggacaggaca aaaagggcca caatgccgtg
     2521 cacggacgcg catcctaatg ggtgggcagg ccgcccgctg cactgcaaaa ccccggggtt
     2581 ctatgtaaga atctccgggt ccttggagga ggaactggct cgatgacagt ggacatcggg
     2641 acgctcgata cgtttcccaa gctcctgctg catcacgcgc aggtgcgcgg cgacctgcct
     2701 gcgatccgcg agaaggacct gggcatctgg cagacctgga cctggcgccg gttcgccgat
     2761 gaagtgcgca cgctggcctg cgcgctcgcc gaccaggggc tgaagcgcgg cgaccacatg
     2821 gcgctggtgg gcgacaaccg cccgcgcatc tacgccgcga tgtgcgccgc gcaatgcctc
     2881 ggtgcgattc ccgtgccgct gtaccaggat gccgtcgccg ccgagatggc gttcccgatc
     2941 cagaacgccg agatcgagca cgcgctcgcc gaggaccagg agcaggtgga caagctgctc
     3001 gagatcctgc cgcagtgccc gacgctcaag cgcatcatct acgacgagcc gcgcggcatg
     3061 cgctcgtatg cgcagcgcga gctcacgagc tacgacgcgc tgctcgaatc gggccgtgcg
     3121 gcgctcaagc gcgatcccgc gtttctcgaa gccgagatcg ccaagggccg cggcgaggat
     3181 accgcggcga tgttcttcac ctccggcacc accggcacgc ccaaaggcgt ggtgctgacg
     3241 caccacgcgc tcatcgaccg cgcgaaggcc gccgccgagc tcgagggcct gggcgatcgc
     3301 gacgtggtgc tcgcctacct gccgccggcg tggatcggcc agaacatctt ttcctatgcg
     3361 cagcccttcg tcaccggcta ttgcatctgc tgcccggagt cggccgagac ggtgatgacc
     3421 gacatgcgcg agatcggccc gacgtactat ttcgccccac cgcgcgtgct ggaggcgctg
     3481 ctcacgcagg tgtcgatccg catggaggac gcgggcgcgg tgaagcgctg gctctaccgc
     3541 aggttcatgg acgtggcgcg gcgcgtcggc gggatgcttc tcgacggcaa gccggtaggc
     3601 atcgccgacc ggctgcgcta cgggatcggc gacctgctgg tctacggacc gctcaggaac
     3661 gtgctcggca tgagccgggt gcgcgttgcc tacacggcgg gcgaggcgat cggcccggac
     3721 ctgttcaagt tctaccgctc gatcggcatc aacctgaagc agctctacgg ctcgaccgag
     3781 accagcgtgt tcgtgtgcgt gcagcccaac ggccaggtga agtccgatac cgtcggcccc
     3841 gcggtgccgg gcgtcgagct caagttcacg cccgagcgcg agcttctcat ccgctcgccg
     3901 ggtctgttca aggagtacta caagaacccg caggcgacga tgcaggcgaa ggaccgcgag
     3961 ggctggttcc acaccggcga cgccgggtac gtcgaccccg acgggcacct gaaaatcatc
     4021 gaccgcgtaa aggacgtggg caaacttgcc gacggcacgc tgttcgcgcc gaagtacctc
     4081 gagaacaagc tcaagttctt cccctacatc caggaggccg tgaccttcgg ccaccagcgc
     4141 gaccgggtct gcgcgttcat caacatcgac gccgaggcgg tgggcaactg ggcggagaag
     4201 cgcaacatgc cgtattcggg ttacgtcgac ctcgcctcgc gcgacgaggt ctacgagctg
     4261 gtgcgcgagt gcgtcgagcg ggtgaacgcc gacctcgcgc aggagcccga ggtcgccaac
     4321 tcgcagatcc accgcttcct catcctgcac aaggtgctgg atgccgatga cgaggagctc
     4381 acgcgcacgc gcaaggtgcg ccgcggcttc gtggcgcaga agtacgccac gctggtcgac
     4441 gcgctctacg gtggcaagga cgcggtgcac gtcgaggcgc aggtgaagta cgaggacggg
     4501 cgcacgggaa cgatttccgc cgacctcaag atccgcgacg ccaagacctt ccgccccgag
     4561 gcggccaagc gcgccgcatg agccgcgccg agcacattgg cgagacggtg ctgtcggtgg
     4621 agaaggtgtc gctcgccttc ggcggcgtca aggcgctaca ggaggtctcc ttcgacgtgc
     4681 gcgagcacga ggtgcgggcc atcatcggcc cgaacggcgc cggcaagtcc tcgatgctga
     4741 acgtgatcaa cggcgtgtac cacccgcagc agggcaccat ccgcctgcgc gccaacgggg
     4801 cgtggaagga attccacgac atggactcgc acgaggcggc ctcgatgggc atcgcgcgca
     4861 cgttccagaa catcgcgctc ttcaagggca tgacggtgct ggacaacgtg atgaccgggc
     4921 ggaacctgaa gatgcgctcg agcttcatcg agcaggcgat ctggatcggc cgcgcgcggc
     4981 gcgaggagct cgcgcaccgg cacgccgtgg aggagatcat cgacttcatg gagatccagc
     5041 acatcaggaa gaccccggtg ggcaagctgc cctatgggct gcaaaagcgc gtcgagctgg
     5101 cgcgcgcgct cgccgccgag ccgcggctgc tgctgctcga tgagccgatg gcgggaatga
     5161 acctcgagga gaagcaggac atgtgccgct tcgtgctcga cgtcaatgac cactacggca
     5221 cgacgatcgt gctcatcgag cacgatatgg gcgtggtgat ggacatctcc gaccgcgtgg
     5281 tggtgctcga ctacggccgc aagatcggcg atggcacgcc cgacgaaatc cgcggcagcc
     5341 agaccgtgat cgacgcctac ctcggagtcg cccattgacc aatgtctcgt tcttcttcga
     5401 ggtgctgatc ggcgggctgc tcgcgggggt catgtactcg ctggtggctt tagggttcgt
     5461 gctcatctac aaggcatccg gcgtgttcaa cttcgcgcag ggcgcgatgg tgttcctcgc
     5521 tgcgctcacc ttcgtgtcgg tggtgaagat gggcgtgaac ttctggctgg cgctggtcat
     5581 cacgctggcg gtgatggtgg tgctcggcat cggcatcgag aagatcgtgc tgcggccgct
     5641 ggtcaaccag ccgcccatca cgctcttcat ggcgaccatc ggcctgacgt tcgtgctcga
     5701 gggcctgtcg cagatggtgt ggggctcgca gccgcatgcg ctcgagctcg gcatcaagga
     5761 cgtgccgatg caatggctgg cggacgcaac gggcatgttc atcagccagt tcgatctgtt
     5821 cgcctccggc atcgccgcgg tgctggtcgc gtcgctcgcc ctgttcttcc agttcacccg
     5881 cgtgggtcgc gcgctgcgcg cggtggccga cgatcaccag gcggcgctcg cagtgggcat
     5941 tccgctgaag cagatctggg cgatcgtgtg ggcggtggcc ggcttcgtgg ccctggtcgc
     6001 cggcatgatg tggggcgcga ggaacggcgt gcagtacgcg ctgacgttca tcgcccttaa
     6061 ggcgctgccg gtgctgatcc tcggcggttt cgagtcgatt cccggcgcga tcatcggcgg
     6121 cctgatcatc ggcgcctccg agaagctggc cgaggtgtac atcgggccga tggtcggcgg
     6181 cggcatcgag tcgtggttcg cctatgtcat cgcgctgctg ttccttctcg tgcggccgca
     6241 agggctgttc ggcgagaaga tcatcgacag ggtctagcca tgctgtatcg cgaagccggg
     6301 cagttcaagt ccacctacgc cgaagaccag cagatcttcc cgatccgcca ggaccgcatc
     6361 gccgtcgcgc tgctgctcct ggtggcgttc gtcgcagtgc ccgtgttcgc caatgaatac
     6421 tggctgtcgg cgattctgac gccgttcctc atcttcgcgc tcgctgcgct cgggctcaac
     6481 atcctcaccg gctacgcggg gcagctctcg ctcggcacgg ctgccttcat ggcggtgggc
     6541 gcgttcatgg cctacaactt cgtgctgcgc ctgccctggc tcggcatcat tccgagcttc
     6601 gttcttgccg gcctgtgcgc ggccgcggtc ggcatcgtgt tcgggctgcc gagcctgcgc
     6661 atcaagggct tctatctcgc ggtggcgacg ctcgcctgcc agttcttcgt gctgtgggcg
     6721 atccagcgca tcggctggtt tacgaacaac agcccgtcgg gcgtgatcac ggcgcagacg
     6781 gtggagatcc tcggctatgc gttcgacacg ccggagtcga agtacctgct gacgctcggt
     6841 attgtcgccg tgcttgcgct gctggcgaag aacctgatgc gcagcgaaac cggccgcgcc
     6901 ttcatggcgg tgcgcgacat ggacgtggcg gcaagcgtga tcggcatccc gatgatgcag
     6961 accaagctcc tcgccttcgc catcagctcg ttttactgcg gcgtggcggg ggcgctgtat
     7021 gcttatacct atctcggcac cgtcgagccc gaggctttca cgctcgatct gtcgttccgt
     7081 atcctgttca tgatcatcat cgggggcgtg gggtcgatcc tgggctcgtt tctgggagcg
     7141 gcgttcatca tcctgctgcc gatcttcctc agcgtgctgt ttggcgtggc ttcgcacacg
     7201 ttcggcctgg acatctcgca cgccatcgtt tccaacctcg agctgatgat tttcggcggg
     7261 ctgatcatct tcttccttat cgtcgagccc catggcctgg cgcgcctgtg gcagatcgcg
     7321 aaggaaaagc tgcgcctgtg gccgttcccg cactagacac tcggaataga ttggctatac
     7381 tcgccgttcc actatatgga ttaggccccg gcatgttctg gggggtcgaa aaaatgaagg
     7441 aggagaccag atgaaacgca ccacattcgc cctggcgctc gcgctcgcgg ccgggttctc
     7501 cggggccgtc ctggcgcagg ccaaggaaca gttcgtgccg gccaatttct actgggtcgg
     7561 cccctacgcc cccggcggtt cgggcttcgg cggcggcatc atcgactaca tgcagatgct
     7621 caacgagcgc gacggcggga tcaacggcgt caagctcacc tgggagaagt gcgagaccga
     7681 gtacaacaac gcgcgtggcg tggaatgcta cgaacgctcg aagaacaagg ggccgacggg
     7741 cgccacggtc atccaccctc tgtcgaccgg cattacctat tccctcatcg agaaggcgac
     7801 cacggacagg attccgatca tctccatggg ctatggccgc gcctcggctg ctgacgggcg
     7861 cgtgtttcct tatgtgttcc ctctgatcac tacctactgg tcgcaggcca gttcgatggt
     7921 gaatttcctc ggcaacagag ccggcggcat ggacaagctg aagggcaaga agatcgtgct
     7981 gctgtaccac gactcggcct acggcaagga gccgattccg attctcactg atctgtcgaa
     8041 ggatttcggg tatcaactga cgacgatcgc ggtgccgcac ccgggcaacg agcagcaggc
     8101 gcagtggctg cagatccggc agttgcagcc ggactgggta atcctgtggg gctggggcgt
     8161 gatgaacccg acggcgatca agactgccgc aaggacggga tatccgcgcg agaagatggt
     8221 gggcgtgtgg tggtccggcg cggaggaaga tgtgatcccc gcggggtccg ccgccaaggg
     8281 ctacatctcg gccggcttca acctgcccgg ctcgaacttc ccggtgatgc gcgacatcca
     8341 gaagtacgtg tacgccaagg gcaagggcga gttcgacgac aagtcgcgct tcggctcgat
     8401 ctatcacacg cgcggtgtcg tggcgggcat tgtcaccacc gaggcgattc gcgcggccca
     8461 ggccaagttc ggcaagggca agccgattac cggcgagcag atgcagtggg gcatcgagaa
     8521 cctgaagatc gacgagaagc ggctgaagga gctgggcgcg gtcggcctga tgccgccgat
     8581 cgaggtgtcc tgcttcaatc acgaagggtc cggattggtc aagtaccagc aatgggacgg
     8641 aagcaagtgg ataccgatca cggattgggt tgagcctgac tacaagctgg tccgcgccaa
     8701 ggtcgaggcg gacgcggcgc agtacgcgaa ggaaaagggc attacgccgc gcgactgccc
     8761 gaagggcggc tgagccggaa ccgcgcgaac gcaacggccg ccgcgaggcg gccgtttttt
     8821 cgttcatggc caccgccgca ttcctctccg tcagcaacgt cgaggtcatc tacgaccacg
     8881 tgatcctcgt cctgcgcggc gtatcgctgc aggtgcagga gggccagatc gtgacgctgc
     8941 tcggcgccaa cggcgccggc aagaccacca cgctgaaggc gatctcgaac ctgctgcgcg
     9001 ctgagcgcgg cgatgtcacg aaggggtaca tcgagttcca gggccagcgc atcgacaagc
     9061 tcaacccgtc cgagctggtg aagcgcggca tctgccaggt gatggagggc cggcactgct
     9121 tccagcacct gacggtggag gagaaccttc tcaccggcgc ctacacgcgc ggcaacgccg
     9181 atctgagcga ggacctcgag aaggtgtacc gctacttccc gcgcctgaag gagcgcagaa
     9241 aggcgcagtc aggctacacc tcgggcggcg agcagcagat gaccgcgatt ggccgcgcgc
     9301 tgatggcgcg cccgaagatg atcctgctcg acgagccgtc gatggggctt gcgccgcagc
     9361 tggtcgagga gatcttcgag atcgtgaaga gcctcaacca gaaggagaac gtgagcttcc
     9421 tcctcgccga gcagaacacc aacatcgcgc tcaggtacgc cgactacggc tacatcctgg
     9481 agaacggccg cgtggtgatg gacggtgagg gcagatcgct cgccgagaac gaggacgtga
     9541 aggagttcta cctcggtttc tccaccggcg ggcgcaagag cttccgcgac gtgaagtcct
     9601 accgcagaag gaagcgttgg ctctgagtcc gttctacgac gcgctggaga cgcgccccgc
     9661 cgaggcgcgc gaagccgcgc tgatggccgc cctgccgaag cacctcgcgc acgccaaggc
     9721 gaacgccccc ggctgggcgc gcatcctgaa ggacgtggac gccgcgtcgg tgcgctcgcg
     9781 cgaggcgctc gcgaagttgc cggtgacccg caagtcggag ctcgccgagc tgcagaaggc
     9841 gcatccgccg ttcggcgggc tcaacgcgac gccggtggac aagctcgccc gggtcttcgc
     9901 ttcgcccggg ccggtctacg accccgaggg aggcggcgcg gactggtggc gcacggcgcg
     9961 ctcgctgtac gcggcgggct tccggccggg cgagctcgtg ctgaacggtt tttcgtacca
    10021 cttcacgccg gccgcttcga tggtggagca cggtgcgcgc gccatcggct gcaccgtcgt
    10081 gccgggcggc acggggcaga ccgagatgca ggccgcgacg atccgcgacc tgcgcgtcac
    10141 cgccttcggc gggacacctt ccttcctcaa gctcatcatc gaaaaggccg acgagctgaa
    10201 ggtcgacgtg tcctcgctca gcaaagcgat cgtcggggcg gagtatctgc cgccggcgct
    10261 ccgccaggcg atgggcgcgc gcggcatccg cgcgatgcag ctctacgcct ccgcggacct
    10321 cggcctcatc gcctacgaaa gcgcgatgcc cgacggggcc atcaacgaag ggatggttct
    10381 cgacgaaggg ctgatcctcg agatcgtgcg gccgggcacc ggagatccgg tgcccgcggg
    10441 cgaggtgggt gaggtcgtcg tcacctcttt taacaaggac tatccgctga tccgcttcgc
    10501 caccggcgat ctgtcggcgg tgctcgccgg caagagcccc tgcgggcgca ccaacacgcg
    10561 catcaagggc tggatgggac gcgccgacca gaccaccaag gtgcgcgcca tgttcgtcac
    10621 gcccaagcag gtcggcgagg tgctcggccg gcacaaggag gtgctgaagg cgcgcctcgt
    10681 cgtcgcgggc gagaccggca acgacagcat gacgctgaag tgcgaggtgc gcgagcgcgc
    10741 cgcaggcctc gccgaggcga tcgtcgcctc gatccgcgat gtcaccaagc tgcgcggcga
    10801 agtcgagctg gtgtcgccgg ggaccctgcc gaacgacggc aaggtcatcg acgacacccg
    10861 caagtacacc taagttattt caccgcaaag cagtagaaca ggccgtcgcc gccggtcgcc
    10921 tgcagcgcct tctggctgca gccgatcgtc gggtgggcgg aattccacga cgtcgcccac
    10981 gcgtggtcga tcgggccggc gcggtcgtgg tgcccggtca tcgccgagcc gtcgccgctc
    11041 ttggtccagt tgccgcaggt ctggtccggg aaccggccgc ccgccgttgg cggatgcgcg
    11101 gtgccgtcgg gccgcgagcc ggtgaggatg tcgtgcttgt tgggcttctc ggtgcggccg
    11161 ttcaccatct ggcccttctc gtccagcgcg gtttccttgt tcacgttggc gcgagccgaa
    11221 tgcaggtcct tgacgtcgcg tgccacgcgc acgcccttgg cgttgtgcca gggcccgctg
    11281 ccgatgcggt cgcgcgcgtg caccaccttc gggtcgttca gcgcgttgcc ctgcgtgctc
    11341 aggtaggcgc gccaggtgcg gtcgcccgcg cccgccgccg cggcgagcgt ctggcagtgc
    11401 ttgtcggcgc cttccaggcc gccgaggtcg gcgccgttgc cgggaccggc actcgtgatg
    11461 aagaacgaca tttcgccgct ctggcccggc atcatggcgc aaccggcgag gacgaacatg
    11521 ccggctgtaa tcaataaggt ccgcttcggc acgctatgca tagcaagtgc tcctcctgtg
    11581 ggtttgactg cgaggcgcag actagcgcga aagcacgtgc cggtgaacgg ccttttccat
    11641 ttttcgcgca acgtgttact tgagcggttc cgcgatggaa tagattctcg ctgccgcgtg
    11701 ttgcggcgcg acagctaggg cttgcgcgcc gcgccgacgg gcagctcgtc gacgcggcgc
    11761 atttccgcat tccgcagcac ggccgggtcg gcaaggaaag cgttcaggaa gccgagcgaa
    11821 tcggcgcccc agaacagctc gccgtctgcc tcgtaggtcg gcacgccgaa cacgccgcgc
    11881 gcgatggcgc cctcggtgtt ccggcgcaat gtctccttga cctcggggga agcgagcttc
    11941 gcctcgtcca ccccgagatc cttcgccagc gtcatgaacg cggccggatc ggcggcttcc
    12001 gcgccggtgg tccagatcgc ctcgaagatg cgttccacgt tcttgcggct gacgcccgcg
    12061 gcaatcgcca gccgcaggtg gtgcagcggg ttgaacgggt gcgacgcggg aaagcggaac
    12121 ggcacgccga gactttgcgc ccaccacgtg caccagcggt agctccagag gcgcttggcg
    12181 gggatctccg cgggaccctt ctggccgatg tggttgagaa tccccgcgaa cagaaccggc
    12241 gtcaactcga gcgtgacgtc gcgcggcagc tcgtgcaggc gcttcaccgc gatgtaggaa
    12301 aaaggcgaaa tgaaatcgaa gtacagggtt accggacgca tgccgggtcc tccttgcgca
    12361 cggggagctc ggccagcacg gcttcgcgct ccgcgtcact catcgcgccc cagcgcgcga
    12421 tctcgtcgag cgtccgctgg cagccgcggc agaagcggtt ctgcgcgtcc atcacgcaga
    12481 tcttgttgca gggcgacttc acgccgcctg cttcgccgcg atcgccctgg cggccttcga
    12541 ctgcggctcc ttgccgatga cgccgcagat ccactgcccg gtggcgacca gcttggcgag
    12601 atcgacgccc gtgtcgatgc ccaggccgtt cagcatgtac accacgtcct cggtggcgac
    12661 gttgcccgag gcccccttgg cgtaggggca gccgcccagc cccgccaccg acgaatcgaa
    12721 ggtcgcgacg cccagttcga gcgatgcata gatgttggcg agcgcctggc cgtaggtgtc
    12781 gtggtagtgg ccggcgaggc gctcgagcgg aactgcgcgc gcgcaggctt cgatcatcgc
    12841 cttcgttttc cccggcgtac cggtgccgat cgtgtcgccg agcgagatct cggagcagcc
    12901 catggcgtca agcgcgcgcg ccacctcggc gaccttcgcc ggcgcgatct cgccctcgta
    12961 ggggcagccg agcacgcagg agatgtagcc ccgcaccttg atgttggcct tgcgcgcggc
    13021 ttcggctacc ggccggaagc gctcgaggct ttcggcgatc gagcagttga tgttcttctt
    13081 ggagaacgac tccgaggcgg cgccgaacac cgcgacctcg gtcgcgccgg cggcgcgcgc
    13141 cgcctcgaag cctttcaggt tgggcgtgag caccgggtag gacacgccgg ccctgcgctg
    13201 gatgccctcc agcacctcgg tgtggtcggc catctgcggg atccacttcg gcgagacgaa
    13261 ggcggtcgct tccactgccg gcaggccggc gtcggcgagc cgggcgatca gctcgagctt
    13321 caccttcgtc ggcacttcca tcttctcgtt ctgcagtccg tctcgcggcc ccacttcgac
    13381 gatgcgcacc ttcttcggca ttgcggccat ggctactcca gagtcagcag ttcggcgccc
    13441 tcgggcacct gctcgccggc ggcgaagtgg atcgccttca ccaccccgtc ggccggcgcg
    13501 ctgatggtgt gctccatctt catcgcttct aggatgagga gcggggcgcc cttttcgacc
    13561 tttgccccgg gcttcaccag gagcgcgatc accttgcccg gcatcggcgc cgcgaggctg
    13621 cccggggcgc tttcgtcctc gtccgcaggc aggccgtctt ccagccgcaa tacggcgcgc
    13681 gcgccgcccg ccgagacatg ccagtcgtgc gccacgtgca ccgcgcgcgc ctcgaacacg
    13741 cggccgtcga gctgcaggcg caggctcccc ggcgccgtgc gctcgcccgc gagcgcatac
    13801 tcgcggccgt cgatcgtgag cccataggcg ccgggccgga actgcaatgt gacctcgtgg
    13861 cgcgcgccgg gctccgcgaa cacgaagtgg tggtgcgagc cctggttgag gcgccagccg
    13921 tccacgctgt cccagggcga gtacgggtcg cccgagcccg cggcgcgcgc acgcgcctcg
    13981 tcggcctctt ccagcagctc ggccatcgca gcggccgcga gcagctcgtg cgaggcgggc
    14041 tgcgcctgcg ggaagagctc ggcgcggctg cgctcgatga gcccggtgtc gagatccgcc
    14101 gtggcgaacg cccggctgcg caccacgcgc tcgaggaaca cgacgttggt ctgcacgcct
    14161 gcgatttcca ctgcggcgag cgcggcgcca aggcgcgcga gcgccgctgg ccggtcctcg
    14221 ccccaggcga tgagcttcgc gatcatcggg tcgtagtacg gcgtgatcgc cgctccgggc
    14281 tcgacgccgg tgtcgacgcg cacccccggc gtggcttccg gaaagcgcaa
//
LOCUS       DBNHKE010000131        22685 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1079825, whole genome shotgun sequence.
ACCESSION   DBNHKE010000131 DBNHKE010000000
VERSION     DBNHKE010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 22685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1079825"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC16O9_08665"
     CDS             <1..642
                     /locus_tag="AC16O9_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6516726.1"
                     /translation="LLASFISPLTNRRKDAYGGTIENRMRWPLEVFRACREAWPQAKP
                     MSVRISATDWAPGGLSGEDLVAMTKMLKGAGCDLIDVSTGQTVPHQRPVYGRMYQASY
                     ADWVRNEIGIATMTVGAVTTADQVNTLLAAGKADLVALARPHLANPYFTLQASAWYQH
                     MGQYWPPQYLPGRDQAFRNAARERAELTDTRLRARPASHEVKDDPKAGVKRAA"
     gene            696..1253
                     /locus_tag="AC16O9_08670"
     CDS             696..1253
                     /locus_tag="AC16O9_08670"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6516727.1"
                     /translation="MKRCAWARNPLAVAYHDREWGVPVRGDRRLFELLILEGAQAGLS
                     WDTILQKRAGYRKAFAGFDPAKVARFGARKKRALMADAGIVRNRLKIEAAVANARAFL
                     RVQEEYGSFARYAWGFVGGKPLVARRRNLRGVPARTELSDALSKDLKRRGFRFVGSTI
                     VYAFMQATGMVNDHTTDCFRWKELR"
     gene            1235..1720
                     /locus_tag="AC16O9_08675"
     CDS             1235..1720
                     /locus_tag="AC16O9_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516728.1"
                     /translation="MERIALALLLAAAPAWGFDANGVALGASEAEVRAAFTGARCKPM
                     DWQTNAADRRCDAAAIRFGGAEARITFFLKAGAVQAFDLRFDDKDLRAVVEHLRQHYG
                     RPDAEGTETFRRRGDARSVYKVRWKKGADRAELSSMASRRRVDLNVWRGNFDTEIYRF
                     R"
     gene            complement(1713..2480)
                     /locus_tag="AC16O9_08680"
     CDS             complement(1713..2480)
                     /locus_tag="AC16O9_08680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR6516729.1"
                     /translation="MASFRSGHRLRAKRWQRAWFALAERVPPLRWLYLARKAWLIRAR
                     STHYAQWGEDVVLEQFLGGLAPGDACFIDVGCFHPKKYNNTWRLYRRGFRGLNIDVDQ
                     VKLDVFDLVRPGDENLCCAIAEGAGEATLYSFGHYSVLTTLDPQKAQDYRRLGYECVE
                     RPVRVRTLTDVFEASRFRSNRIALLSVDVEGHEEWVLRSLDFARYRPRLILCEFHVPT
                     LARMQADPKYRLLTGELRYELVNWTGLTAFFMDRDAT"
     gene            2556..3164
                     /locus_tag="AC16O9_08685"
     CDS             2556..3164
                     /locus_tag="AC16O9_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR6516730.1"
                     /translation="MSLRANLDELAFRAASAWSDARRGVRLAWYRADHEAMAFALMIG
                     SVLLVFGFGLHAVFARHERARELVRETERKNLECLAKNVYYEARGEPAEGQYAVAEVT
                     MNRKASRRYPATVCEVVHEKRWDAIRKRYVGAFSWTEFYAVPEPAGEAWTLAQKVAED
                     VYYQRAPARVDGATYYHATSIEPSWARTQERVARIGRHVFYR"
     gene            complement(3155..3625)
                     /locus_tag="AC16O9_08690"
     CDS             complement(3155..3625)
                     /locus_tag="AC16O9_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR6516731.1"
                     /translation="MKKWLILAVTHGAALLGGVALGIYLLPILTAPAAPSANEVSAAA
                     GQAEFKGEFRRDLKDSDFLHWGEGTVSVSRGAIALMGAIAPGPDYKLYLSPEFVETEA
                     DFKRLKPKMARVGDVKTFENFIVPVPASIDPKAYTTVIVWCESFGQFITAARYR"
     gene            3667..3993
                     /locus_tag="AC16O9_08695"
     CDS             3667..3993
                     /locus_tag="AC16O9_08695"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6516732.1"
                     /translation="MSIDYAQSAPPRAEIDALAGPTLLEFGSPTCGHCRRAQPLIASA
                     LAAHPGVRHLKFADGRGRRLGRSFGVKLWPTLVFLVDGQERVRLVRPVAAGEIERALQ
                     AIDPGA"
     gene            complement(4077..4793)
                     /gene="arsN2"
                     /locus_tag="AC16O9_08700"
     CDS             complement(4077..4793)
                     /gene="arsN2"
                     /locus_tag="AC16O9_08700"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HIR6516733.1"
                     /translation="MTANPALNRSHRRQALHVSVLLRAAALVGVRTRSMVSVDERQLA
                     PDLGPITTVAAGATASPRKVGLAHAGMRERSLTDQEDAMNLFRNPPAPQVKSLLTESQ
                     LPSSDLSPKDLEHFFGCGATQAPKGVVGLEIYGTVALLRSLAVAKDCRGIGCGKALVA
                     EAERYAESKGVSELFLLTTTAERFFERLGYRRSPRENAPQAIRQTKEFSGLCPSSSVF
                     MAKALAADPGVQPTPASGRR"
     gene            complement(4797..5597)
                     /locus_tag="AC16O9_08705"
     CDS             complement(4797..5597)
                     /locus_tag="AC16O9_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6516734.1"
                     /translation="MNFETLVVTETDGVAHIELNRADKANAMNGAMWKDLRSAFDWLS
                     AGRARVGVLSARGKHFTAGIDLELLVAMQSEIAGLPEGRRQERLGKIIVELQDAISAA
                     ESCRKPILAAIHGACMGGGIDLVTACDMRYTTKDARFSVKEIDLAIVADVGTLQRLPR
                     LVGEGLARELAFTGREFDGNEAHAMKLVNQVFADRESLMTAVLKTAAEIASKSPLTIR
                     GIKDTLNYSRDHSVAQGLSYVAAKNAAILLSSDLAEAMSAAQEKRKAR"
     gene            complement(5718..5894)
                     /locus_tag="AC16O9_08710"
     CDS             complement(5718..5894)
                     /locus_tag="AC16O9_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516735.1"
                     /translation="MVYVRIRYEETELRGRAKRLGATWRKPQKLWEITHRDSKRPGIE
                     DRMVEAAGPGTIHD"
     gene            complement(5930..6880)
                     /locus_tag="AC16O9_08715"
     CDS             complement(5930..6880)
                     /locus_tag="AC16O9_08715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR6516736.1"
                     /translation="MRCVQYERFGDESVLQVREVPAPLPAAGQVQVRVAFASLNPVDF
                     KLRSGLLRFIGKPARPAITGKDFAGTVSALAMEVAGLRPGQRVFGSVNAMGGAGSCAE
                     FVALDAGLVAPTPEGVSDEVAACLPVASGTAYQALVTQARLRAGQSVLVTGASGGVGS
                     SAVQIARSIGARVTGVCGTSNLEYVRSIGADEVVDYRRADWRAPGRVYDVIFDAAGAS
                     SFGAARPHLAPAGWYINTYPPPSLMAAAPFVRLFSRQHAVPFMLKTTAAQLAELARLA
                     QSGVLRPRIARTVALEEVAAAQRDMQEGRVSGKVCVRLAA"
     gene            complement(6883..7566)
                     /locus_tag="AC16O9_08720"
     CDS             complement(6883..7566)
                     /locus_tag="AC16O9_08720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516737.1"
                     /translation="MPAVLVTGAGRGIGLATAQAFALLGWKVWSLDKAFGREVVGERV
                     DYDLRKLGGIGKMVGRLGAVDTLVNNAGVLYCDPYDAIPETHFEEILEVNLRAPVALI
                     EALAPQMKTRRSGRIVNVGSVAAFTGHPDLWYGATKAALLNITKSYAGALGKHGILVN
                     AVAPGPTLTAMYEQLPQSRKDGVMRSVHAGRPCSPEEVARAIVWLGSASPDYVSGSTL
                     DVNSGSYPR"
     gene            complement(7568..8527)
                     /locus_tag="AC16O9_08725"
     CDS             complement(7568..8527)
                     /locus_tag="AC16O9_08725"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6516738.1"
                     /translation="MALTSAPTAARTQPAARLKGVLSPVITPFKADLSPDAERFVRHC
                     KWLLKSGCTGLAIFGTNSEANSLSLEEKLTLLAALVHAGVPGSALMPGTGHCALPATV
                     RLTANAVKLGCAGVLMLPPFYYKGVSDEGLYRYFSEVIERVGDERLRVYLYHIPPVSQ
                     VPITLKLIERLLAKYPGIVAGAKDSSGDWNNTKAMLEEFGKAGFDVFPGSEQFLLDGM
                     RHGGAGCISATANVNPGPIDHVFRNWRSGEADKLQAGISATRKIMQKFPMIPALKSVT
                     AHFGNDPEWRRVRPPLVELTAEQEAALLPELKAAGFSMPGLAG"
     gene            8599..9744
                     /locus_tag="AC16O9_08730"
     CDS             8599..9744
                     /locus_tag="AC16O9_08730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR6516739.1"
                     /translation="MTRLQRAVNIEDLRRMARSRLPRSVFDFFDGGAEDETTLRGNRA
                     AFERVRLLPRVLVDVHAVDTSVQLFGARSKLPMAIAPTGAIGAGRHDADLMIAKAARA
                     AGIPYSLATPATNSIEEIAEQVGGRLWFQLYLLKNRDFRMQLVARAKAAGYEALLVTV
                     DLTTGGKRERDLRNDFAAPFVPTWRNSRDVLLKPAWLAGMARRGVPQMKNLEGMLHKP
                     PRLTDVAASVGRELDASFAWDDVKSLRDAWPGKLVLKGIVHPADAERAVAAGCDGVVV
                     SNHGGRQLDGAIATADALPLVARAAGGKLTVLVDGGVRRGVDLLKARALGAHGVLTGR
                     ATLFGCMAGGEAGAKRAIEILGGEFERAMMLCGVARAEDIGADLLAP"
     gene            9741..10925
                     /locus_tag="AC16O9_08735"
     CDS             9741..10925
                     /locus_tag="AC16O9_08735"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HIR6516740.1"
                     /translation="MNLPVLVLGGGIGGLAAALALAKKGCRVRLFEQGSALKEIGAGI
                     QLGPNVYHMFDVLGLTAAIERWSAHPENLIMMDALTGEQVTRLPVGSDAFRTRFRGYP
                     YGVIHRGDLYQVLLEACRAENNVELNEKSKGLSFEDKGDRVVLRMEGGVAIEGACLVG
                     ADGLWSRVRAQILGDGKPRVSGHIAYRAVLPRAEVPDDLWQNNVVLWAGPKTHLVHYP
                     LRRGELYNLVAVFHSDRYEEGWDVFGDTEELARKFENERPEVKRLLQKINAWKMWVLC
                     DREPARHWSKGRATLLGDAAHPTLQYLAQGANMAIEDAVVLAAYAQITGFDWEKTFKL
                     YEDARYKRTARVQIMSRYYGDAYHAAGVVRELRNDILAPAPGAAPDFEGVAWLYDGIT
                     LP"
     gene            complement(10922..12310)
                     /locus_tag="AC16O9_08740"
     CDS             complement(10922..12310)
                     /locus_tag="AC16O9_08740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6516741.1"
                     /translation="MHPALVILAVGLSPALVGEFTPHLQRLAARGGMRPLRTVTPAVT
                     CSVQSTLVTGLAPSGHGIVANGWYFRDLSEVWLWRQSNRLVAGEKLWEAAKARDASFT
                     CAKMFWWYNMYASADWSATPRPMYPADGRKIPDHYAHPQRLHDELDAKLGPFPLFKFW
                     GPLTDISSTEWIAQATLHVMQTRNPTLALTYLPHLDYNLQRLGPDLSHPRVQQDLKEL
                     DAVCGTLIDAASASGRDVIVVSEYGITPVTDAVHVNRALRKAGLIAVRPEEHGREILD
                     PGASAAFAVADHQIAHVYVNDRSRAAEVRALLEKLDGVERVLDEAGKRAFGLDHPRSG
                     ELVAISKAERWFSYYYWLDERLAPDFARTVDIHRKPGYDPVELFFDPALAAPKLASAW
                     RLARRKLGFRTLMDVISLKDTALVKGSHGRLTDDPRHGPLVISSKGDLLPAGEVEAIA
                     FKQLVLDHLFAR"
     gene            12343..13200
                     /locus_tag="AC16O9_08745"
     CDS             12343..13200
                     /locus_tag="AC16O9_08745"
                     /inference="COORDINATES: protein motif:HMM:NF035938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HIR6516742.1"
                     /translation="MSESATTLLRAWLLRQLDAKQGAWLDEQIAALRKDAADTTLDVA
                     LGMIPRRLGRGELALTDADLAAAGKAVPGWDPRGWGVAEAARILALVSLPAAGKPFAE
                     RFRSLCSTADVAELATLYRGLPLYPDSAALEPQVGEGLRSNMRVVFEAIAHRNPYPKA
                     HFDDHRWNHMVLKALFIGSPLAPIQGLDERANPQLARIMLDFAHERWAAGRPVPFEIW
                     RCVGPFAEGQAIEDLARVLERGEGIERRAAALALAASPDRRAAELLAAVPALAGEISG
                     KRLSWATLQ"
     gene            13203..14156
                     /locus_tag="AC16O9_08750"
     CDS             13203..14156
                     /locus_tag="AC16O9_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6516743.1"
                     /translation="MDEPIEDPAMMLIDPHAHMISRTTDDYEAMAAAGVVAVIEPAFW
                     IGQPRTNVGSYLDYLSSIVGFERFRASQFGIRHYCTIGLNSKEANNEALAEGVMELIP
                     RFALKEGVVAIGEIGYDEQSALEDKYFRLQLELAKKLDLPVMIHTPHRDKKRGTSRSM
                     DVCDEHGLDPRKVVVDHNNEETVKETLERGYWAAFSIYPSTKMGNARMVEILRQYGAK
                     QVIVDSACDWGISEPLGVAKTAKLALQQGISEADVRLACYQNALEAYGQSGQMKEEDW
                     LNPVAIDQRAVFEGNSILRGGREPKVEAGKSRRTMDDLIIE"
     gene            14361..15659
                     /locus_tag="AC16O9_08755"
     CDS             14361..15659
                     /locus_tag="AC16O9_08755"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6516744.1"
                     /translation="MKFAKVGGAFSAALLLAVAGGTAVAAGPKIKLEPLVTAVNTPLA
                     MVQPAGDSRMFIIEQNGRVKILEGGKLRPTPFLDIRSKIPALLHDFDERGLLGIAFHP
                     NYKSNGKFYVAYSAHLDYQSDLGQMLWYSHSNVVEEYTVSKSDPNAADPSSARRILST
                     PWPQFNHNGHWIGFGKDGKLYVSMGDGGYANDWGIGHNPAIGNGQDMSTLLGKILRID
                     VDARTGDKPYGIPSDNPFVNDRNAKPEIWASGLRNPWRCSFDTGSGQLFCADVQQNSY
                     EEVNIIAKGQNMGWRRMEATHCFDYLKPDNHPASCDKGGLTGPILEYQNCTAKPQGCK
                     GISVTGGYVYQGKQAALKGKYIFGDWSKSFAEMDGQIFIATNSGGKWSMEVAEVTNMK
                     GKLPYVLAFAQDANGEVYALTSITTGPNGSLDTIYKIVAQ"
     gene            15734..16084
                     /locus_tag="AC16O9_08760"
     CDS             15734..16084
                     /locus_tag="AC16O9_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6516745.1"
                     /translation="MRNTRFTGLVAIALACALAAATAWGEDTPVFDKAYLSNKKNIAA
                     GREVWDNQCRHCHGAAADPGKAPKLKPGQLAPDFIYDRVTYGFGKMPGWKDVFSVQQR
                     KAVVAYIKSGDFSP"
     gene            complement(16059..16415)
                     /locus_tag="AC16O9_08765"
     CDS             complement(16059..16415)
                     /locus_tag="AC16O9_08765"
                     /inference="COORDINATES: protein motif:HMM:NF026420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HIR6516746.1"
                     /translation="MRYVLMLLGLLMVAFAGVQYNDPDGLLWACIYFVPAAWAFAAAF
                     RPAVVRSLTAERLLWATVAAGVGTMVIYWPTVPHFWRKDVWWVEETAREGMGVMVGFA
                     VLVVVLAASMARNRRS"
     gene            16416..17594
                     /locus_tag="AC16O9_08770"
     CDS             16416..17594
                     /locus_tag="AC16O9_08770"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6516747.1"
                     /translation="MTHSTRARTTTHWQRFAVPYEFPVVFTEGVFDPENPALRDVLCR
                     LEPAKRHRAVFFVDDGLGEGLLAAIRTYAGRHRDAIELACAPVAVPGGEKIKTELHFV
                     ESMQQKLFELHLDRHSYVIAVGGGAVLDAVGLVAATTHRGVRHVRIPTTVLAQNDSGV
                     GVKNGVNLQGVKNFVGTFAPPFAVLNDLEFVLTLPERDKIAGMAEAVKVALIRDAEFF
                     AWIEHHMDELATFERPAMAYMIRRCAELHMRQIGQGGDPFEMGSARPLDYGHWSAHKL
                     ESLTRHHVRHGEAVAIGMALDARYSVLAGLLPAGEEERLCALLEYLGFTLWHPALAKT
                     GVNGEWTILEGLKDFQEHLGGELTITLLAGIGRGVEVHAIDVERMREAMAWLRQRAGD
                     "
     gene            17594..18778
                     /gene="eboE"
                     /locus_tag="AC16O9_08775"
     CDS             17594..18778
                     /gene="eboE"
                     /locus_tag="AC16O9_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HIR6516748.1"
                     /translation="MHLGGLGHLTYCTNIHAGEALAEVRAGLEKHLAPIKAAVSPERP
                     LGVGLRLGHAAAEGLRDSRALAALKRYLDEGGYYVFTLNGFPYGPFHGRAVKENAYRP
                     DWSEPERLAYTDHLADILAALLPAGQHGSVSTVPCTFKPWAEGRIDAVVENLVRHVAH
                     LVGIAKKTGQTIALALEPEPCCYLETIDEAVEFFESRLFGRASVARLASLAGLSGAGA
                     ESAMRRHIGLCYDVCHAAVEFEDPKASIGRLRASGIGIFKLQLSAALRVARFGAASAR
                     QLGEFAEPVYLHQVVRRANGALTRYVDLPQALAEAGSAEGSEWRVHFHVPIFLERMEH
                     FDTTQAFLAEILRLHRQAPISAHLEVETYTWDVLPQAYRKADLSAAIARELNWVKTQL
                     QQ"
     gene            18775..19623
                     /locus_tag="AC16O9_08780"
     CDS             18775..19623
                     /locus_tag="AC16O9_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR6516749.1"
                     /translation="MKLGTALRLGRVSNLPTVWTNALAGAVLAGAAGFGAEFALLLLA
                     FTLFYTGGMFLNDAFDAAIDARERPERPIPSGAATRSEVLGWGFGQMAAGVALLAVVG
                     IAPALAGLTLAVAITYYDWNHKTNPLSPVVMGLCRVFIYLGAGLAYTLALPGALWIGA
                     ALLLCYLIGLTYVAKQENLERVENLWPLAFLAAPVAYGAWHALEAPVTGLYWLAFTGW
                     MLWALWFLRRRRKGDIPRAVVSLIAGISLLDALLIAGAGSPGLALAALAGFGVTLFFQ
                     RYIAGT"
     gene            complement(19625..20500)
                     /locus_tag="AC16O9_08785"
     CDS             complement(19625..20500)
                     /locus_tag="AC16O9_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516750.1"
                     /translation="MRSTIAVAVVLVLAMPLAPARAAEGWRFGLAGEAVHDDNATRGL
                     LDGAKSDNIVVLEGSATRSFLLSPSSGALVRASARYSHFADIKDISNLALIGRAAWRY
                     QAGTGFSAPWYELAGQAQLLRHADSELRDGSIVSIDASAGSHLTDRMRVAGGVGFDKR
                     SGGGSVGLYELSTNRIWATLDLRLGARNTAYARLTREAGDHVFSSGSTSGLTAVWEDD
                     PALREPLGLAVANTYRVDATALIVELGFNYPLSRAQALDFSLVRIDSEMDEGLLQGNS
                     YSATQLRATYLYRFQ"
     gene            20570..21271
                     /locus_tag="AC16O9_08790"
     CDS             20570..21271
                     /locus_tag="AC16O9_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6516751.1"
                     /translation="MSKRFNDAEDPRRAFLLKALAAGLLAAGGVPAVRAQLFGRKPQK
                     LPAGQSVYEATGAVTVNGQAATSATRIGATDRIETGRGAQLIFVVGFDAFLLRESSRL
                     ELSGVSGIVNTMRLATGALLSVFGAGAKRIVTTTSASGIRGTGIYVESEPERSYVCTC
                     YGDVLISAVDDASISERIVSTHHDAPRYVMKGRIEPAPFINHTDVELALIEALVGRTP
                     PFSLFDEDYGGARRY"
     gene            complement(21289..21480)
                     /locus_tag="AC16O9_08795"
     CDS             complement(21289..21480)
                     /locus_tag="AC16O9_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HIR6516752.1"
                     /translation="MPRAVSLAAQIKQALGIEAELAKGANGVFDVAADGKLVFSKHRD
                     QRYPEPEEIIGALKARQRA"
     gene            complement(21528..>22685)
                     /locus_tag="AC16O9_08800"
     CDS             complement(21528..>22685)
                     /locus_tag="AC16O9_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6516753.1"
                     /translation="GQPPALRKLTFASNVIQLGSPVQLELSARSPEGLARAVQALQAE
                     LRRIDGVSDVRDDRDPGKQEVQFELKPSARALGISLESLALQVRAAFFGAEALRVQRG
                     RDEVRVYVRLPRGERASLADLGRYRIRAPSGAFVPLHEVAELKLDYGPSTIVRRHGRR
                     VSTVFAEIDNRVVTGQQVNARLTGEVIPALQQEIPGLTYAMGGEQREQARTLPSLARN
                     FVLAMFCMYALLALAFRSYLQPLIVLASIPLGLVGAVLGHLALGLPMGLTSLFGVVGL
                     AGIIVNGSLVLIDFINEERARGKPPAEAIRVACKGRFRPIVLTAVTTFLGIFPLIVER
                     SIQAQFLIPLAIAIAAGVLVGTLIQMLLTPALAMAVEDLRARVAGSVQRAA"
BASE COUNT         3590 a         7752 c         7885 g         3458 t
ORIGIN      
        1 ctgctcgcga gcttcatctc gccgctcacc aatcggcgaa aagacgccta cggcggcacg
       61 atcgagaacc gcatgcgctg gccgctggaa gtgttccgcg cctgccgcga ggcctggccg
      121 caggcgaagc cgatgtcggt acgcatctcg gcgaccgact gggcgcccgg cgggctctcg
      181 ggcgaggacc tcgtggcgat gacgaagatg ctcaaaggtg cgggctgcga cctgatcgac
      241 gtctctactg gtcagacggt gccgcaccag agaccggtct acggccgcat gtaccaggcg
      301 agctacgctg attgggtgcg caacgaaatc ggcatcgcca ccatgacagt gggcgcggtg
      361 accacggccg atcaggtaaa tacgctcctg gccgcgggca aggccgacct cgtcgcgctc
      421 gcgcgcccgc acctcgccaa cccctacttc acgctgcagg cctcggcctg gtaccagcac
      481 atgggccagt actggccgcc gcagtacctc ccgggccgcg accaggcgtt ccgcaacgcc
      541 gcgcgcgagc gcgccgagct caccgacacc agactgcgcg cgcggccggc gagccacgag
      601 gtcaaggacg atcccaaagc cggcgtgaag cgggcagctt aaatggggac agacctcatt
      661 tacgcttgcg cctgacgaaa atgaggtctg tccccattaa acgttgcgcc tgggcaagga
      721 atcctctcgc ggtcgcctat cacgaccgcg agtggggcgt gccggtgagg ggcgatcggc
      781 gcctgttcga gctcctgatc ctggagggcg cgcaggcggg cctgtcgtgg gacacgatcc
      841 tgcagaagcg cgccggctac cgcaaggcgt tcgcggggtt cgatccggcg aaggtggcga
      901 ggttcggcgc gaggaaaaag cgcgccctga tggccgacgc cggcatcgtc aggaaccgcc
      961 tgaagatcga agcggcggtg gcgaacgcga gggcgtttct ccgggtgcag gaggaatacg
     1021 gcagcttcgc gcgctatgcc tggggcttcg tcggcggcaa gccgctggtc gcgcgccgcc
     1081 gcaacctgcg cggcgtgccc gcgcgcaccg agctctccga cgcgctgtcc aaggacctca
     1141 agcggcgcgg ctttcgcttc gtcggctcga ccatcgtgta cgctttcatg caggcgacgg
     1201 gcatggtgaa cgaccacacc acggactgct ttcggtggaa agaattgcgc tagcgctgct
     1261 gctggccgcg gcgcccgcct ggggcttcga cgccaatggc gttgccctcg gcgcgagcga
     1321 agccgaggtg cgcgcggcgt tcaccggggc gcgctgcaag ccgatggact ggcagaccaa
     1381 cgccgccgac cggcgctgcg acgccgcggc catccgcttc ggcggcgccg aggcgcgcat
     1441 caccttcttc ctcaaggcgg gcgcggtgca ggcattcgac ctgcgcttcg acgacaagga
     1501 cctgcgcgcc gtggtcgagc acctgcgcca gcactacggc cggccggacg cggaaggcac
     1561 cgagacgttc cggcggcgcg gcgacgcgcg ctcggtgtac aaggtgcgct ggaagaaggg
     1621 cgcggaccgt gcggagctga gctcgatggc ctcgcgccgg cgggtggacc tcaacgtctg
     1681 gcgcggcaat ttcgataccg agatctaccg cttcaggtag cgtcgcggtc catgaagaag
     1741 gcggtgagcc cggtccaatt gacgagctcg tagcggagct cgccggtgag cagccggtac
     1801 ttcgggtccg cctgcatgcg cgcgagcgtc ggcacgtgga actcgcacag gatgagccgc
     1861 gggcgatagc gtgcgaagtc gagcgagcgc agcacccact cctcgtgccc ctcgacgtcg
     1921 acgctgagca gcgcgattct gttgctgcgg aagcgcgagg cttcgaatac gtcagtgagc
     1981 gtgcgcacgc gcaccggtcg ctcgacgcac tcgtagccga ggcgccggta gtcctgcgcc
     2041 ttctgcggat cgagcgtggt cagcaccgag tagtggccga aggagtacag cgtcgcctcg
     2101 cccgcgccct cggcgatggc gcagcacagg ttttcgtccc ccgggcgcac caggtcgaag
     2161 acgtcgagct tcacctggtc gacgtcgatg ttgagacccc gaaagccgcg gcggtacagg
     2221 cgccacgtgt tgttgtactt cttcgggtgg aagcagccga cgtcgatgaa gcacgcatcc
     2281 ccgggcgcga gcccgccgag gaactgctcc agcaccacat cctcgcccca ctgcgcgtag
     2341 tgcgtgctgc gggcgcggat cagccaggcc ttgcgtgcga ggtacagcca ccgtagcggc
     2401 ggcacgcgtt ccgcaagcgc gaaccacgcc cgctgccagc gctttgcgcg cagccggtgc
     2461 cccgagcgga aacttgccat gcgttcagga tgtccaagaa aacgccattg tgcccttgac
     2521 agcggccgcc cgcgccgcga acactgcgcc ccgccatgtc cctgcgcgcg aacctcgatg
     2581 agcttgcctt ccgcgccgcg tcggcctgga gcgacgcccg ccgcggcgtg cgcctggcct
     2641 ggtaccgcgc ggaccacgag gcgatggcat tcgcgctcat gatcggctcg gtgctgctgg
     2701 tgttcggctt cggcctgcac gcggtgttcg cgcgccacga gcgcgcacgg gagctcgtgc
     2761 gcgagaccga gcgcaagaac ctcgaatgcc tggcgaagaa cgtgtactac gaggcgcgcg
     2821 gcgagccggc cgagggccag tacgcggtgg ccgaagtgac gatgaaccgc aaggcgtcac
     2881 ggcgttaccc ggccacggta tgcgaggtgg tgcacgagaa gcgctgggac gcgatccgca
     2941 agcgctacgt gggcgcattc tcgtggaccg agttctatgc cgtgcccgag cctgcgggcg
     3001 aggcctggac gttggcgcag aaggtggccg aggacgtgta ctaccagcgc gcgccggcgc
     3061 gcgtcgacgg cgccacctac taccacgcca cctccatcga gccgagctgg gcgcgcacgc
     3121 aggagcgcgt cgcgcgcatc ggccggcacg tgttctaccg gtagcgcgcg gcggtgatga
     3181 actggccgaa ggattcgcac catacgatca ccgtggtgta ggccttcgga tcgatggacg
     3241 cgggcaccgg gacgatgaag ttctcgaagg tcttcacgtc gccgacgcgc gccatcttcg
     3301 gcttgaggcg cttgaagtcg gcctcggtct cgacgaactc gggcgagaga taaagcttgt
     3361 agtcgggccc gggggcgatt gcccccatca gcgcgatggc gccgcggctc accgagaccg
     3421 tgccttcgcc ccagtgcagg aagtcgctgt ccttcaggtc ccgcctgaat tcgcccttga
     3481 actcggcctg tcccgccgcg gcgctgacct cgttcgcgga gggcgccgcc ggcgccgtca
     3541 ggatgggcag caggtagatg ccgagcgcga cgcccccgag cagcgcggcg ccgtgcgtga
     3601 ccgccagaat cagccatttc ttcatgcagc cctgcttgcg gcgattttgg cataggcttc
     3661 gcggcgatga gcatcgacta cgcccaaagc gcgccgccgc gcgccgagat tgacgcgctg
     3721 gcgggcccga cgttgctcga gttcggcagc ccgacctgcg ggcactgccg gcgcgcgcag
     3781 ccgctgatcg cttccgcgct cgccgcgcac cccggcgtgc ggcacctcaa gttcgccgac
     3841 gggcgcgggc gccggctggg ccggtccttt ggcgtgaagc tgtggccgac gctcgtgttc
     3901 ctagtggacg ggcaggaacg cgtgcgcctg gtgcggccgg tcgcggcggg cgagatcgag
     3961 cgggcgctgc aggccatcga ccccggcgcc tgacgtccgg catccgctgc gtgcccttgc
     4021 tacgcgtcgg cgcgttgccg ggcggcgctt acgcaccatg gaggcccgca acatggtcag
     4081 cggcgcccgc ttgcgggcgt cggctgcacg ccggggtcag ctgccaatgc cttggccatg
     4141 aacacggaac tcgatgggca aaggccggag aattccttcg tctggcgaat ggcttgcggc
     4201 gcattctcgc gggggctgcg tctatagccc aagcgctcga agaatcgctc cgccgtggtg
     4261 gtcagcagga acagctccga cactcccttg gactcggcat agcgctcggc ttcggccacc
     4321 agcgccttcc cgcagccgat gccgcggcaa tccttggcga cggcgaggga gcgaagcagg
     4381 gctaccgtgc catagatctc gaggccgacg actcctttgg gggcctgcgt agcgccacac
     4441 ccgaagaagt gctcgaggtc cttgggcgaa aggtcagagc tcggcaactg ggattccgtc
     4501 agcaggctct ttacttgtgg tgccggtgga ttgcggaaca ggttcattgc gtcctcctga
     4561 tcagtcagcg aacgctcgcg catgccggcg tgggccaggc cgaccttgcg tggcgaggcg
     4621 gtcgcgcccg cggccacagt ggttattggg cccaagtcag gcgccagttg ccgttcatcc
     4681 acactgacca tcgaccgcgt cctgacccct acgagagctg cggccctgag cagcacggag
     4741 acatgcaaag cttgccggcg gtgcgaccgg ttgagcgccg ggttggctgt catctcttat
     4801 cgcgctttgc gcttctcttg cgccgcggac atcgcctcgg cgaggtcgga ggacagcaat
     4861 atggctgcat tcttggccgc gacgtaagag aggccttgtg ccaccgagtg atcccggctg
     4921 tagttgagcg tgtccttgat accgcgaatt gtcagcggcg acttgctcgc aatctcggcg
     4981 gccgttttca ggacagccgt cataaggctc tccctgtccg caaagacctg gttcacaagc
     5041 ttcatcgcat gggcttcgtt gccgtcgaac tctctgcccg taaacgcaag ttctcgtgcg
     5101 agtccttcgc ccacgagcct tggaagccgc tggagtgtgc cgacgtccgc cacgatcgcc
     5161 agatctatct cctttaccga aaaccgcgcg tccttggtgg tgtatcgcat gtcgcaggcg
     5221 gtgaccagat cgattccccc tcccatgcac gcgccatgta ttgcggcgag aatgggtttt
     5281 ctgcacgact ctgcggcgct tatggcgtcc tgcaattcaa cgatgatttt ccccagtcgt
     5341 tcctgcctcc tgccttcggg caggccggca atctcggatt gcattgccac gagcaattcc
     5401 aggtcgattc cggccgtaaa gtgcttgcct cgggcgctca gcacgccaac acgcgctcgg
     5461 ccggcgctca accagtcgaa agccgatctc aagtccttcc acatcgcccc gttcatcgcg
     5521 ttcgccttgt ccgcgcgatt caattcgata tgggcgactc catccgtttc ggtgacgacc
     5581 aaggtctcga agttcacgct caactccctg ggcagactcc gcctaacgtg gattaggcag
     5641 cagaactgcg gaccgaaaac tatccatatg tagattgaac catttaccta tagataccgc
     5701 catatgccgg catgtatcta gtcatggata gttcccggcc ccgccgcctc caccatccgg
     5761 tcctcgatcc cgggccgctt gctatctcga tgagtgatct cccacagctt ctgcggcttg
     5821 cgccaggtcg cgcccagccg cttggcgcgc ccgcgcagtt ccgtttcctc gtaccggatg
     5881 cgcacgtaga ccacgtcgtc gtcctgccgc cgcggcgcgg ttgacgcgcc taggccgcca
     5941 ggcgcacgca gactttgccg ctcaccctgc cttcctgcat gtcgcgctgg gccgccgcga
     6001 cctcctcgag cgcaacggtg cgcgcaatgc gcggccgcag gacgcccgac tgcgccaggc
     6061 gcgccagctc ggcgagctgc gccgccgtcg tcttcagcat gaacggcacg gcgtgctgcc
     6121 gcgagaacag gcgcacgaac ggggctgccg ccatgagcga cggcggcggg taggtgttga
     6181 tgtaccagcc ggccggcgcc aggtgcggcc gcgccgcacc gaagctcgag gcgcccgcgg
     6241 cgtcgaagat gacgtcgtag acgcggcccg gcgcgcgcca gtcggcgcgg cgatagtcga
     6301 cgacttcgtc ggcgccgatc gagcgcacgt actcgaggtt cgaggtgccg cacacgccgg
     6361 tgacgcgcgc cccgatcgag cgcgcgatct gcaccgcgct ggagcccacc ccgcccgaag
     6421 cacccgtgac gagcaccgac tggccggcgc gcaggcgcgc ctgggtgacg agcgcctggt
     6481 aggcggtgcc ggaggcgacc ggaaggcagg ccgccacttc gtcgctcacg ccctcggggg
     6541 tcggcgcgac gagcccggcg tcgagggcga cgaactcggc gcagctgccg gcgccgccca
     6601 tggcgttcac cgagccgaag acgcgctggc cgggccgcaa tccggcgacc tccatggcga
     6661 gcgcgctgac cgtgccggcg aagtccttgc cggtgatcgc cgggcgcgcg ggcttgccga
     6721 tgaagcgcag cagccccgag cgcagcttga agtcgacggg attgaggctc gcgaacgcga
     6781 cgcgcacctg gacctggccc gcggcgggca ggggtgccgg gacctcgcgc acctggagca
     6841 cggactcgtc gccgaagcgc tcgtactgga cgcagcgcat ggctaccgcg gatacgagcc
     6901 gctgttcaca tccagcgtgc tgcccgacac gtagtccggg ctcgcgctgc cgagccacac
     6961 gatcgcccgc gccacttcct cgggcgagca cggccgcccg gcgtgcaccg agcgcatgac
     7021 gccgtccttt ctcgattgcg gcagctgctc gtacatggca gtgagcgtgg ggccgggcgc
     7081 caccgcgttg accaggatgc cgtgcttgcc cagtgcgccc gcatagctct tggtgatgtt
     7141 gagcagcgcg gccttggtcg cgccgtacca gaggtccggg tgcccggtga acgcggcgac
     7201 cgaaccgacg ttgacgatgc gccccgacct tcttgtcttc atctgcggcg cgagcgcctc
     7261 gatgagcgcg accggcgcgc gcaggttgac ctccagtatt tcctcgaagt gcgtttcagg
     7321 gatcgcgtcg tacggatcgc agtagagcac gccggcgttg ttcaccagcg tgtcgacggc
     7381 gccgagcctg ccgaccatct tgccgatgcc gccgagcttg cgcaggtcgt agtccacgcg
     7441 ctcgccgacg acttcgcgcc cgaacgcctt gtcgagcgac cagaccttcc agccgaggag
     7501 cgcgaacgcc tgcgcggtgg caagcccgat gccgcggccc gcgcccgtga cgaggaccgc
     7561 gggcacgcta gccggcgagg cccggcatcg agaacccggc ggccttcagc tccggcagga
     7621 gcgcggcttc ctgctccgcg gtgagctcga cgagcggcgg gcgcacgcgc cgccactcgg
     7681 ggtcgttgcc gaaatgcgcg gtcaccgact tcagcgccgg gatcatgggg aatttctgca
     7741 tgatcttgcg cgtcgcgctg atgcccgcct ggagcttgtc agcttcgccg gagcgccagt
     7801 tcctgaatac gtggtcgatc ggcccggggt tgacgttggc ggtggcgctg atgcagccgg
     7861 cgccgccgtg gcgcatgccg tcgagcagga actgctcgct gcccgggaac acgtcgaagc
     7921 cggccttgcc gaactcctcc agcatcgcct tggtgttgtt ccagtcgccc gaggaatcct
     7981 tcgccccggc gacgatgccc ggatacttgg cgagcagccg ctcgatcagc ttcagcgtga
     8041 tcggcacctg cgacaccggc gggatgtggt acaggtacac gcgcagccgc tcgtcgccca
     8101 cgcgctcgat cacctccgag aagtagcggt acaggccttc gtccgacacc cccttgtagt
     8161 agaacggcgg cagcatcagt acgcccgcgc agcccagctt cacggcgttg gcggtgagcc
     8221 gtaccgtggc gggcagggcg cagtgcccgg tgcccggcat cagcgccgag ccgggcacgc
     8281 ccgcgtgcac cagcgcggcc agcagcgtca gcttttcctc cagcgacagc gagttggcct
     8341 cgctgttggt gccgaagatc gccagtcccg tgcagccgct cttcagcagc cacttgcagt
     8401 ggcgcacgaa gcgctcggcg tcgggcgaga ggtcggcctt gaacggcgtg atgacgggcg
     8461 agagcacccc cttcaggcgg gcggcgggct gggtgcgggc ggcggtcggg gcggacgtga
     8521 gggccatgtc gggttcgcgg tcgattttgt gagacgacat tatgcgcgcg cccggggcgc
     8581 tcggtacact tcccggccat gacccggctc cagcgcgcgg tgaacatcga ggacctgcgc
     8641 cgcatggcgc gctcgcgcct gccgcgctcg gtgttcgact tcttcgacgg cggcgccgag
     8701 gacgagacga cgctccgcgg caaccgcgcc gccttcgagc gcgtgcggct gctgccgcgg
     8761 gtgctggtcg acgtgcatgc ggtcgacacc agcgtgcagc tgttcggggc gcgctcgaag
     8821 ctgccgatgg cgatcgcgcc caccggggcg atcggcgccg ggcgccacga cgccgacctg
     8881 atgatcgcga aagcggcgcg cgccgcgggc attccgtatt cgctcgcgac ccccgccacc
     8941 aactcgatcg aggagatcgc ggagcaagta ggcgggcgcc tgtggttcca gctctacctg
     9001 ctgaagaacc gcgacttccg catgcagctc gtcgcgcgcg cgaaggccgc gggctacgag
     9061 gcgctgctcg tcaccgtcga tctcaccacc ggcggcaagc gcgagcgcga cctgcggaac
     9121 gacttcgccg cgccgttcgt gccgacctgg cgcaactcgc gcgacgtgct gctcaagccc
     9181 gcctggctcg ccggcatggc gcgccgcggc gtgccgcaga tgaagaacct cgagggcatg
     9241 ctgcacaagc ccccgcgcct caccgacgtc gcggcctcgg tcgggcgcga gctcgatgcg
     9301 tccttcgcct gggacgacgt caagtcgctg cgcgacgcct ggccgggcaa gctcgtgctg
     9361 aagggcatcg tgcacccggc cgacgccgag cgcgccgtgg ccgcgggctg cgacggcgtg
     9421 gtggtgtcga accacggcgg tcgccagctc gacggcgcca tcgccaccgc ggacgcgctg
     9481 ccgctcgtcg cgcgcgccgc gggcggcaag ctcacggtgc tcgtcgacgg cggcgtgcgc
     9541 cgcggcgtgg atctgctcaa ggcgcgcgcg ctcggcgcgc acggcgtgct caccgggcgc
     9601 gccacgcttt tcggctgcat ggcgggcggc gaggcggggg cgaagcgcgc catcgagatc
     9661 ctcggcggcg agttcgagcg cgccatgatg ctgtgcggcg tcgcgcgcgc cgaggacatc
     9721 ggcgccgacc tgctcgcgcc gtgaacctgc cggtcctggt gctgggcggc ggcatcggcg
     9781 gcctcgccgc cgcgctcgcg ctggcgaaaa agggctgccg cgtgcgcctg ttcgagcagg
     9841 gctcggcgct caaggaaatc ggcgccggca tccagctcgg cccgaacgtc taccacatgt
     9901 tcgacgtgct cggccttacc gccgcgatcg agcgctggtc ggcgcatccc gagaacctga
     9961 tcatgatgga cgcgctcacc ggcgagcagg tgacgcgcct gccggtgggc agcgacgcct
    10021 tccgcacgcg ctttcgcggc tatccgtacg gcgtcatcca ccgcggcgac ctgtaccagg
    10081 tgctgctgga agcctgccgc gccgagaaca acgtcgagct gaacgagaag agcaagggcc
    10141 tctccttcga ggacaagggt gatcgcgtgg tgctgcgcat ggaggggggc gtagcgatcg
    10201 agggggcgtg tctggtcggc gcggacggcc tgtggtcgcg cgtgcgcgcg cagatcctcg
    10261 gcgacggcaa gccgcgcgtc tcgggccaca tcgcctaccg cgcggtgctg ccgcgcgcgg
    10321 aagttccgga cgatctgtgg cagaacaacg tcgtgctgtg ggcgggaccg aagacgcacc
    10381 tcgtgcacta tccgttgcgc cgcggcgagc tctacaacct ggtggcggtg ttccacagcg
    10441 accgctacga agagggctgg gacgtgttcg gcgacaccga ggagctcgcg cgcaagttcg
    10501 agaacgaacg ccccgaggtg aagcggctgc tgcagaagat caacgcctgg aagatgtggg
    10561 tgctgtgcga ccgcgagccg gcgcggcact ggtcgaaggg gcgcgcgacg ctcctcggcg
    10621 acgcggcaca cccgaccctg cagtacctcg cgcagggcgc caacatggcg atcgaggacg
    10681 cggttgtgct cgccgcctat gcgcagatca ccggcttcga ctgggagaag accttcaagc
    10741 tctacgagga cgcgcgctac aagcgcaccg cccgcgtgca gatcatgtcg cgctactacg
    10801 gcgacgccta ccacgccgcc ggcgtggtgc gcgagctgag aaacgacatc ctcgcgcccg
    10861 cgcccggcgc cgcgcccgac ttcgagggcg tggcctggct ctacgacggc atcacgctgc
    10921 cttagcgggc gaacaggtga tcgagcacca gctgcttgaa ggcgatcgct tcgacttcgc
    10981 cggcgggcag tagatcgcct ttcgagctga tgacgagcgg accgtgccgc gggtcgtcgg
    11041 tgagccgccc gtgcgagccc ttgacgagcg cagtgtcctt gagcgaaatc acgtccatca
    11101 acgtgcgaaa gcccagtttt ctcctggcga gccgccaagc gctggcgagc ttgggcgcgg
    11161 cgagcgccgg gtcgaagaac agctccaccg ggtcgtagcc gggcttgcgg tggatgtcga
    11221 ccgtgcgcgc aaaatcgggc gccagacgct cgtccagcca gtagtagtag ctgaaccagc
    11281 gctcggcctt cgagatcgcc acgagttccc cggagcgcgg gtgatcgagg ccgaacgcgc
    11341 gcttgccggc ctcgtccagc acgcgctcca cgccgtcgag cttctcgagc agcgcgcgca
    11401 cttcggcagc gcggctgcgg tcgttcacgt agacgtgcgc gatctggtgg tcggccaccg
    11461 caaacgccgc gctcgccccc gggtcgagga tctcgcgccc gtgctcctcg gggcgcaccg
    11521 cgatcagccc cgccttgcgc agcgcgcggt tgacgtgcac ggcatccgtc accggcgtga
    11581 tgccgtactc cgagaccacg atcacgtcgc gcccgcttgc gcttgccgcg tcgatcagcg
    11641 tgccgcacac ggcgtcgagc tccttcaggt cctgttgcac gcgtgggtgc gaaagatccg
    11701 gcccgaggcg ctgcaggttg taatccaggt gcggcaggta ggtgagcgcg agcgtcgggt
    11761 tgcgcgtctg catcacgtgc agcgtcgcct gcgcgatcca ttcggtggag ctgatgtcgg
    11821 tgagcggccc ccagaacttg aacagcggaa agggcccgag cttcgcgtcg agctcgtcgt
    11881 gaagccgctg ggggtgcgcg tagtggtccg ggatcttgcg cccgtcggcc gggtacatcg
    11941 gccgcggcgt cgcgctccag tcggcgctcg cgtacatgtt gtaccaccag aacatcttcg
    12001 cgcaggtaaa gctcgcgtcg cgcgccttcg cggcctccca gagcttttcg ccggcgacga
    12061 ggcggttgga ctggcgccac agccagactt cggacaggtc gcggaaatac cagccgttgg
    12121 cgacgatgcc gtggccgctc ggcgccaggc ccgtcaccag cgtcgactgc acgctgcagg
    12181 tcaccgcggg cgtcacggtg cgtagcggtc gcatgccgcc gcgcgccgcg agccgctgca
    12241 ggtggggcgt gaattcgccg acgagcgccg gcgacaggcc gaccgcgagg atgacgagcg
    12301 cgggatgcat gcggcgtaga atatcagcgc catggaggag ggatgagcga gtccgcgacg
    12361 acactgttgc gcgcatggct gctgcgccag ctggacgcga agcagggcgc ctggctggac
    12421 gagcagatcg cggcgctgcg caaggacgcg gcggacacca cgctcgatgt ggcgctcggc
    12481 atgatcccgc ggcgcctcgg caggggcgag ctcgcgctca ccgacgccga tctcgcggcg
    12541 gccggcaagg cggttcccgg ctgggacccg cgcggctggg gcgtcgccga agccgcgcgc
    12601 atcctggcgc tcgtttcgct gcccgccgca ggcaagccgt tcgccgagcg cttccgctcg
    12661 ctgtgcagca cggcggatgt cgcggagctg gcgacgctgt atcgcgggct gccgctctac
    12721 ccggactcgg cggcgctcga gccgcaggtc ggcgaaggcc tgcgcagcaa catgcgcgtg
    12781 gtgttcgagg ccatcgcgca ccgcaatccc tatcccaagg cgcacttcga cgatcaccgc
    12841 tggaaccaca tggtgctgaa ggcgctgttc atcggcagcc cgctcgcgcc gatccagggg
    12901 ctggacgagc gcgccaaccc gcagctcgcg cgcatcatgc tcgacttcgc ccacgagcgc
    12961 tgggccgccg ggcggccggt gccgttcgag atctggcgct gcgtcgggcc gttcgccgaa
    13021 ggccaggcga tcgaggacct cgcgcgcgtg ctggagcgcg gcgagggcat cgagcggcgc
    13081 gccgccgcgc tcgcgctcgc ggcgagcccg gaccggcgcg cggccgagct gctcgccgcg
    13141 gtgccggcgc tcgccgggga gatcagcggc aagcgcctca gctgggcgac actgcagtag
    13201 acttggacga gccaatcgag gatcccgcca tgatgctcat cgacccgcac gcgcacatga
    13261 tctcgcgcac caccgacgac tacgaggcga tggcggcggc cggggtcgtc gccgtgatcg
    13321 agcccgcgtt ctggatcggc cagccgcgca ccaacgtcgg ctcgtacctg gattacctgt
    13381 cctccatcgt cggcttcgag cgcttccgcg ccagccagtt cggcatccgc cactactgca
    13441 ccatcggcct gaactccaag gaagcgaaca acgaggcgct ggccgagggc gtgatggagc
    13501 tcattccgcg tttcgcgctg aaggagggcg tggtggcgat cggcgagatc ggctacgacg
    13561 agcagtcggc gctcgaggac aagtacttcc gcctgcagct cgagctcgcc aagaagctcg
    13621 acctcccggt gatgatccac accccgcacc gcgacaagaa gcgcggcacc tcgcgctcga
    13681 tggacgtgtg cgacgagcac gggctcgacc cgcggaaggt ggtggtcgac cacaacaacg
    13741 aggaaacggt gaaggagacg ctcgagcgcg gctactgggc ggctttttcg atctacccga
    13801 gcaccaagat gggcaacgcg cgcatggtgg agatcctccg gcagtacggc gcgaagcagg
    13861 tgatcgtcga ttcggcctgc gactggggca tatccgagcc cctgggagtg gcgaagacgg
    13921 ccaagcttgc actgcaacaa ggtatctccg aggcagatgt caggctggcc tgctaccaga
    13981 acgcgctcga ggcttacggc cagagcggcc agatgaagga agaggactgg ctgaaccccg
    14041 tcgccatcga ccagcgtgca gtctttgaag gcaattccat cctgcgcggc ggccgcgagc
    14101 cgaaggtcga ggcgggcaag agccggcgca cgatggacga tttgataatc gagtagcgcc
    14161 gccccgccgt acgcgttgtg cagcgcgtaa acggacacgc cgggcggcgc gttccgcata
    14221 tccaaaccgt atccgatagt cgcgccacga gccagttgta gaaaatcgta aagttttttt
    14281 ttatactccg cccctcccgc cgggaggcgg gcggcgcgct gaaggccgag gctcgttcta
    14341 aatcatgagg aggaggcaac atgaaattcg cgaaagttgg cggggcattt tctgccgcgc
    14401 tgctgctcgc cgtggcaggc gggacagcgg ttgctgcagg ccccaagata aagcttgaac
    14461 ccctggtcac ggccgtcaac acgccgctcg ccatggtgca gccggcgggc gacagccgca
    14521 tgttcatcat cgagcagaac ggccgcgtca agatcctcga gggcggcaag ctccgcccga
    14581 cgccgttcct cgacatccgc agcaagatcc cggccctgct ccacgacttc gacgagcgcg
    14641 gcctgctcgg catcgcattc catccgaact acaagtcgaa cggcaagttc tacgtcgcct
    14701 acagcgcgca cctcgattac cagtccgacc tcgggcagat gctgtggtac tcgcacagca
    14761 acgtggtcga ggagtacacg gtgtccaaga gcgacccgaa cgcagcggac ccgagctcgg
    14821 cgcggcgcat cctgtccacg ccttggccgc agttcaacca caacggacac tggatcggct
    14881 tcggcaagga cggcaagctg tacgtctcga tgggcgacgg cggctacgcc aacgactggg
    14941 gcatcggtca taaccccgcc atcggcaacg gccaggacat gtccacgttg ctgggcaaga
    15001 tcctgcgcat cgacgtcgat gcgcgcaccg gcgacaagcc ctacggcatt ccgtcggaca
    15061 atccgttcgt gaacgaccgc aacgccaagc ccgagatctg ggcctcggga ctgcgcaatc
    15121 cgtggcgctg ctcgttcgac accggcagcg ggcagctgtt ctgcgccgac gtgcagcaga
    15181 acagctacga ggaggtcaac atcatcgcca agggccagaa catgggctgg cgcaggatgg
    15241 aagcgacgca ctgcttcgac tacctcaagc ccgacaacca tcccgcgagt tgcgacaagg
    15301 gcggattgac cggcccgatc ctcgagtacc agaactgcac cgcgaagccg caaggctgca
    15361 agggcatctc ggtgacgggc ggctacgtct accagggcaa gcaggcggcg ctcaagggca
    15421 agtacatctt cggcgactgg agcaagtcgt ttgccgagat ggacggccag atcttcatcg
    15481 ccaccaacag cggcggcaag tggagcatgg aggtcgccga agtgaccaac atgaaaggga
    15541 agctccccta cgtgctggcg ttcgcgcagg acgcgaacgg cgaggtctat gcgctgacct
    15601 cgatcaccac cggcccgaac ggctcgctcg acacgatcta caagatcgtc gcgcagtaag
    15661 catcgggcag tgaaccacgc cgccgcaccg cgccagcggc gcggcggctt tctttcggga
    15721 gtggatttgc agcatgagga atactcgatt tacggggctt gtggcgatcg cgctggcgtg
    15781 cgcactggcc gccgcgacgg cgtggggcga ggacacgccg gtgttcgaca aggcctacct
    15841 gtcgaacaag aagaacatcg ccgccggccg cgaagtctgg gacaaccagt gccggcactg
    15901 ccatggtgcg gccgccgacc ccggcaaggc gcccaagctc aagccggggc agctggcgcc
    15961 ggatttcatc tacgaccggg tgacctacgg cttcggcaag atgccggggt ggaaggacgt
    16021 gttctccgtg caacagagaa aagcggtcgt cgcctacatc aagagcggcg atttctcgcc
    16081 atagaggcgg cgagcacgac gacgaggacg gcaaagccga ccatgacgcc catgccttcg
    16141 cgcgcggtct cctccaccca ccacacgtcc ttgcgccaga agtgcggcac ggtgggccag
    16201 tagatcacca tggtgccgac gccggccgcc accgtcgccc acagcagccg ctccgcggtc
    16261 agcgagcgca ccactgccgg gcggaacgcg gcggcgaacg cccatgccgc gggcacgaaa
    16321 tatatgcaag cccacagcag gccgtccggg tcgttgtact ggacgcccgc aaatgccacc
    16381 atcagcaatc cgagcagcat caggacatag cgcatatgac gcattctacg cgggcgcgca
    16441 ccaccacgca ctggcagcgc ttcgccgtgc cgtacgagtt tcccgtggtg ttcaccgagg
    16501 gcgtgttcga cccggagaac ccggcgctgc gcgacgtcct gtgccgcctc gagcccgcca
    16561 agcgccaccg ggcggtgttc tttgtggacg acggcctggg cgaggggctg ctggcggcga
    16621 tccgcaccta cgccgggcgc caccgcgacg cgatcgagct cgcctgcgcg ccggtcgcgg
    16681 tgcccggcgg cgagaagatc aagaccgagc tgcatttcgt cgagagcatg cagcagaagc
    16741 tctttgagct gcacctcgac cgccattcgt acgtgatcgc ggtgggcggc ggggcggtgc
    16801 tcgacgccgt ggggctggtc gccgccacga cgcaccgcgg cgtgcgccac gtgcgcatcc
    16861 cgaccacggt gctggcgcag aacgattccg gcgtcggcgt gaagaacggc gtcaacctgc
    16921 agggcgtgaa gaacttcgtc ggcaccttcg cgccgccgtt cgcggtgctg aacgacctgg
    16981 aattcgtgct cacgctgccc gagcgcgaca agatcgcggg catggccgag gcggtgaagg
    17041 tggcgctcat ccgcgatgcc gagttcttcg cctggatcga gcaccacatg gacgagctcg
    17101 ccacgttcga gcgcccggcg atggcgtaca tgatccggcg ctgcgccgag ctgcacatgc
    17161 gccagatcgg ccaggggggc gacccgttcg agatgggcag cgcgcgcccg ctcgattacg
    17221 gccactggtc ggcgcacaag ctggagagcc tcacgcgcca ccacgtgcgc cacggcgagg
    17281 cggtggcgat cggcatggcg ctcgatgcgc gctactcggt gctcgcgggg ctgctgcccg
    17341 cgggcgagga agagcgcctc tgcgcgctgc tcgagtacct gggctttacc ctctggcacc
    17401 cggcgctcgc caagaccggc gtcaacggcg aatggacgat cctcgagggc ctgaaggact
    17461 tccaggagca tctcggcggc gagctcacca tcacgctgct cgccggcatc ggcaggggcg
    17521 tcgaggtgca cgcaatcgac gtcgagcgca tgcgcgaggc catggcctgg ctgcggcagc
    17581 gcgcgggcga ctgatgcacc tcggcgggct cgggcacctc acctactgca ccaacatcca
    17641 cgcgggcgaa gcgctcgcgg aggtgcgcgc ggggctggaa aagcatctcg cgccgatcaa
    17701 agccgcggta tcgccggagc ggccgctcgg cgtgggcctg cgcctcgggc acgcggcggc
    17761 cgagggcttg cgcgattccc gggcgcttgc cgcgctcaag cgatatctcg acgagggcgg
    17821 ctactacgtc tttacgctga acggctttcc ctacgggccg ttccacggcc gcgcggtgaa
    17881 ggagaatgcc taccgcccgg actggagcga gcccgagcgc ctcgcctaca ccgatcacct
    17941 cgccgacatc ctcgcggcgc tgctccccgc gggccagcac ggcagcgtca gcaccgtgcc
    18001 ctgcaccttc aagccctggg ccgaagggcg catcgacgcc gtcgtggaga acctggtccg
    18061 ccacgtggcg cacctcgtcg gcatcgcgaa gaagaccggc cagacgattg cgctcgccct
    18121 cgagcccgag ccctgctgct acctggagac catcgacgag gcggtcgaat tcttcgagtc
    18181 gcgcctgttc ggcagggcga gcgtggcccg gctcgcgtcc ctcgccgggc tgtcgggcgc
    18241 cggcgccgaa agcgcgatgc gccggcacat cggcctgtgc tacgacgtgt gccatgccgc
    18301 ggtggagttc gaggacccca aggcgagcat cgggcggctg cgcgccagcg gcatcggcat
    18361 cttcaagctg cagctctccg cggcgctgcg cgtcgcgcgc tttggcgcgg cgagcgcgcg
    18421 ccagctcggc gagtttgccg agccggtcta cctgcaccag gtggtgcgcc gcgcgaacgg
    18481 cgcgctcacg cgctacgtcg acctgccgca ggcgctcgcc gaggcgggct cggccgaggg
    18541 cagcgagtgg cgcgtgcact tccacgtgcc gatcttcctc gagcgcatgg agcacttcga
    18601 caccacgcag gcgttcctcg ccgagatcct gcggctgcac cggcaagcgc cgatcagcgc
    18661 gcacctcgag gtcgagacct acacctggga cgtgctgccg caggcctacc gcaaggcgga
    18721 cctcagtgcc gcgatcgcgc gcgagctgaa ctgggtcaag acgcagcttc agcagtgaag
    18781 ctcggcacgg cgctgcgcct cgggcgcgtc tccaacctgc cgacggtgtg gaccaacgcg
    18841 ctcgccggcg cggtgctcgc gggcgcggcc ggcttcggcg cggagttcgc gctgctgctt
    18901 ctcgccttca cgctgttcta caccggaggc atgttcctga acgacgcctt cgacgccgcg
    18961 atcgacgcgc gcgagcgtcc cgagcggccg attccctcgg gcgcggcgac gcgcagcgag
    19021 gtgctcggct ggggcttcgg gcagatggcg gcgggcgtcg cgttgctggc ggtggtcggc
    19081 attgcgccgg cgctcgccgg cctcacgctc gcggtggcga tcacctatta cgactggaac
    19141 cacaagacca atcccctgag cccggtggtg atgggattgt gccgcgtctt catctacctg
    19201 ggggcggggc tcgcctacac gctcgcgttg cccggtgcgc tttggatcgg cgccgcgctc
    19261 ctgctctgtt acctcatcgg gcttacgtac gtggccaagc aggagaacct cgagcgcgtg
    19321 gagaacctgt ggccgctcgc tttcctggcg gcgccggtcg cctacggcgc gtggcacgcg
    19381 ctcgaggcgc cggtgacggg cctttactgg ctcgcgttca ccggctggat gctgtgggcg
    19441 ctgtggttcc tgcgccggcg ccgcaaaggc gacatcccgc gcgcggtggt gagcctgatc
    19501 gcggggatct cgctgctcga cgcgctgctg atcgcgggcg cggggtcgcc cgggctcgcg
    19561 ctcgccgcac tcgccgggtt cggcgtgacg ctcttcttcc agcgctacat cgccgggacc
    19621 tgagctactg gaagcggtag aggtaggtgg cgcgcagctg ggtggcgctg taggagttgc
    19681 cctgcaacag gccctcgtcc atctccgagt cgatgcgcac aagggagaag tcgagcgcct
    19741 gcgcgcggct caacggatag ttgaaaccga gctcgacgat caacgcggtc gcgtcgaccc
    19801 gataggtgtt cgccacggcg aggcccaggg gctcgcgcag cgccggatcg tcctcccaca
    19861 cggccgtcaa gccgctggtc gagcccgaac tgaacacgtg gtcgcccgcc tcgcgcgtga
    19921 ggcgcgcgta tgccgtgttg cgcgcgccaa gccgcaggtc gagcgtggcc cagatgcggt
    19981 tcgtggacaa ctcgtacagg cccaccgagc cgccgccgct gcgcttgtcg aagccgacgc
    20041 caccggccac gcgcatgcga tcagtgaggt ggctgccggc gctcgcgtcg atggaaacga
    20101 tgctgccgtc gcgcagttcg ctgtcggcgt ggcgcagcaa ctgcgcctgc ccggcgagct
    20161 cgtaccacgg ggcgctgaag ccggttccgg cctgatagcg ccaggcggcg cgaccgatga
    20221 gcgcgaggtt gctgatgtcc ttgatgtcgg cgaagtgcga gtagcgcgcc gacgcgcgca
    20281 ccagcgcgcc gctactcggc gacagcagaa agctccgcgt cgccgagcct tcgagcacca
    20341 cgatgttgtc cgacttcgcg ccgtcgagta gtccgcgcgt ggcgttgtcg tcgtgtacgg
    20401 cctcgccggc gagcccgaag cgccagcctt ccgcggcccg cgccggtgcg agcggcatcg
    20461 ccagcacgag gacgacggct acggcgatgg tggatctcat gtgactttcc ccaagttgtg
    20521 ctccgcgtga taatacgtcg tcattccgct cagcgaaagg gacgcgccga tgtcgaagcg
    20581 attcaacgat gccgaagacc cgcgccgcgc attcctgctg aaggcgctgg ccgcgggact
    20641 gcttgcggcg ggcggcgtgc ccgccgtgcg cgcgcagctc ttcgggcgca agccgcagaa
    20701 gctgcccgcc ggccagtcgg tgtacgaggc gaccggcgcg gtcacggtga acgggcaggc
    20761 ggcgacgagc gcgacgcgca tcggcgccac cgaccgcatc gagaccggca ggggcgcgca
    20821 gctcatcttc gtcgtcggct tcgacgcgtt cctgctgcgc gagagcagcc gcctcgagct
    20881 ctcgggcgtg agcggcatcg tgaacacgat gcgccttgcc accggcgcgc tgctctcggt
    20941 ctttggcgca ggcgcgaagc gcatcgttac cacgacttcc gcctccggca tccgcggcac
    21001 gggcatctac gtcgaatccg agcccgagcg cagctacgtc tgcacgtgct acggcgacgt
    21061 gctcatttcc gccgtcgacg acgccagtat ctcggagcgc atcgtctcga cgcatcacga
    21121 cgcaccgcgc tacgtcatga agggccgcat cgaaccggca ccgttcatca accacacgga
    21181 cgtcgagctg gcgctgatcg aggcgctggt cggccgcacg ccgccgttct cgctgttcga
    21241 cgaagactac ggcggcgcgc gccgctacta attacgccgc ctcgggcgct aggcgcgctg
    21301 gcgcgccttg agggcgccga tgatctcctc aggctcgggg tagcgctggt cgcggtgctt
    21361 ggagaacacg agcttgccgt cggcggcgac gtcgaagacg ccgttcgcgc ctttcgccag
    21421 ctcggcctcg atgccgaggg cctgcttgat ctgggcagcc agactgaccg cccgcggcag
    21481 gtagtttcag gggacgcagt aggtaatgac gaccttcatg cagcccatta tgccgcgcgc
    21541 tggacgcttc cggcaacgcg cgcgcgcagg tcctcgaccg ccatggcgag cgcgggcgtg
    21601 agcagcatct ggatgagtgt gccgaccagc acgcccgcgg cgatggcgat ggcgagcggg
    21661 atcaggaact gcgcctggat gctgcgctcg acgatgagcg ggaagatgcc gaggaaggtg
    21721 gtcacggcgg tgagcacgat cggccggaag cggcccttgc acgccacgcg gatggcctcg
    21781 gccggaggct tgccgcgcgc gcgctcctcg ttgatgaaat cgatcagcac cagcgacccg
    21841 ttgacgatga tgccggccag gccgacgacg ccgaacaggc tggtgaggcc catcggcagg
    21901 ccgagcgcga ggtgcccgag caccgcgccg accaggccga gcgggatcga ggcgagcacg
    21961 atcagcggct gcaggtagga acggaaggcg agcgcgagca gcgcgtacat gcagaacatg
    22021 gcgagcacga agttgcgcgc gagcgaggga agcgtgcgcg cctgctcgcg ctgctcgccg
    22081 cccatggcgt aggtgaggcc gggaatctcc tgctgcagcg ccgggatcac ctcgccggtg
    22141 aggcgcgcgt tgacctgctg gccggtgacc acgcggttgt cgatctcggc gaacacggtc
    22201 gatacgcgac ggccgtggcg gcgcacgatg gtggaggggc cgtagtcgag cttgagctcg
    22261 gcgacctcgt gcagcggcac gaacgcgccc gaaggcgcgc ggatgcggta gcggccgagg
    22321 tcggcgagcg acgcgcgctc gccgcgcggc agccgcacgt acacgcgcac ctcgtcgcgg
    22381 ccgcgctgca cgcgcagcgc ttccgcgccg aagaacgccg cccgcacctg cagggccagc
    22441 gactccaggc tgatgccgag cgcgcgcgcc gagggcttga gctcgaactg cacttcctgc
    22501 ttgcccgggt cgcggtcgtc gcgcacgtcg gacacgccgt cgatgcgccg cagctccgcc
    22561 tgcagcgcct gcaccgcgcg cgcgagccct tcgggggagc gcgcggagag ctcgagctgc
    22621 accggcgagc cgagctggat cacgttggag gcgaaggtga gcttgcgcag ggccgggggc
    22681 tggcc
//
LOCUS       DBNHKE010000132        27159 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1344741, whole genome shotgun sequence.
ACCESSION   DBNHKE010000132 DBNHKE010000000
VERSION     DBNHKE010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 27159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27159
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1344741"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1628)
                     /locus_tag="AC16O9_08805"
     CDS             complement(39..1628)
                     /locus_tag="AC16O9_08805"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR6516754.1"
                     /translation="MSQVIGVPRESAADEKRVATVPEVAQKLIKLGFKVMVQSGAGAG
                     ANFDDASYEAAGAQIAPDAKALWSAADIVFKVRAPTAEEVGLMRGSQTLVSFIWPAQN
                     SELMQSLAARGVTVLAIDSLPRMLSRAQKMDALTSMASISGYRAVVEAANAFGRFFNG
                     QVTAAGKIPPAKVFIAGAGVAGLSAIGTAVGLGAIVRANDTRAEVADQVVSMGGEFVK
                     VDYEEEGAGGGGYAKVMSEGFQQAQREMYAKQAREVDIIITTALIPGKPAPRLITADM
                     VRSMKPGSVIVDMAAEQGGNCELTEAGKAVVKHGVTIIGYTDLPSRLAKQSSTLYATN
                     LFRLAEELCKGKDGVIDVNMEDDAIRGLTVIKQGSITWPPPPPKLPAPPAAAKPAAAP
                     PAPKGHGHGAGAPRSGRAMAITFVVAALLFWFIGANAPASFLNHFTVFVLACFVGYMV
                     VWNVTPALHTPLMSVTNAISSIIAIGALVQIAPPLAAGAGAARPEEWIRWLAVVGVGL
                     TAINMFGGFAVTRRMLQMFRK"
     gene            complement(1726..3228)
                     /locus_tag="AC16O9_08810"
     CDS             complement(1726..3228)
                     /locus_tag="AC16O9_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIR6516755.1"
                     /translation="MRRAAVLLALAAAAAATQAEPLVVGSKRFTESYLLGELIAQTAR
                     AAGAEVELRPGMGGTKILYEALRTGAIDLYPEYTGTIAREILQGETHASLGALNARLA
                     PLGLAASVPLGFNNTYAIGVRAALAERRGLGRISDLKGQRRLRFGFGHEFLARRDGWP
                     GLAAAYGLAQRPRGLDHGLAYEALAAGEVDVIDLYSTDAKIARYAIAILEDDRGFFPA
                     YDAVLLHRTGVPERFPRAWRALRGLEGTIDAERMRRMNAAAELEGKSFADAARLHGAG
                     RAAPPRPGARTLMAALFGPDFWRLTREHLLLVFASLAASVAAAVPLGVAAAKLPRLAQ
                     PILGVVGVIQTIPSLALFAFLIALTGAIGTWPALLALFLYALLPIVRNTHAGLAGIAA
                     GMHQAALALALPRRVRLWRIELPLALPTILAGVKTSAVINVGTATIAAFIGAGGYGER
                     IASGLALNDNVMLLAGAIPAAALALLVQGAFELAERRCAWMREAPGQGKV"
     gene            complement(3225..4982)
                     /locus_tag="AC16O9_08815"
     CDS             complement(3225..4982)
                     /locus_tag="AC16O9_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6516756.1"
                     /translation="MGSLARLARFLAPYRWRVAAALAALLAAAACVLALGQGLKYVVD
                     AGFGSGEPRLLDQALAAAVAVAVTLAIATYFRFYLMMSTGERVVTDLRRAVFDHLLGL
                     SPGFYETARTGELVSRLASDTTVVQQVIGYGLSMFVRNLLMMVGAAAMLFFTSWKLAA
                     LVLAGVPATLVPILLLGRRVRRLSRRSQDRVADVSAHVDEAVHEVRTVQAYAHEDTDR
                     AAFAQHAEAAYAAAAASLGQKSALIATVMLIAFCAVGVILWVGGHDVLAGRLSAGELA
                     AFVFYAGVVATGAGTVSEVWGDLQRAAGATERLFELLDARPAIAAPAAPRPVPAAPRV
                     YEFDAVSFAYAGRPHAAALERFSLRLAAGERVALVGPSGAGKSTVLALLLRFYDPQGG
                     AIRVDGVDLRDMEPRALRGLMAVVPQEPVIFAASAFDNVRYGRPEASRAEVERACEQA
                     FAAEFIARLPQGLDTPLGERGVTLSGGQRQRLSIARALLADRPLLLLDEATSSLDAES
                     ERMVQQALERLEHGRTTLVIAPRLATVQHADRIVVMDRGTIVAQGTHAELMRQGGLYA
                     SLAQLQFLDGAAARVARAA"
     gene            complement(5006..7543)
                     /locus_tag="AC16O9_08820"
     CDS             complement(5006..7543)
                     /locus_tag="AC16O9_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR6516757.1"
                     /translation="MNAPRTLDLILPQARVDAARVAALFGIAKTVYAATLVAGALVVA
                     MLWGETPVAALATWYGVLAAVTLARLGLHRAHAAHPERRTPLAWERRFALGAFTAGLL
                     WALPIAAFFPQAPVLQTAVLLVTAAAVVGAAGVYAASAAAFYGFCLPPILAVVVQFAR
                     QPGEVYPLLALAALVFVAAMVRVYRDMHANVVGALGARAEKEALLERVAQSEARLRDA
                     IEGFPEGIAVFDEDGRVLASNDAYTTVAGEARAAGAAEAPAVRQFRTRDGRWMQGHFV
                     RMERGGAVGVFTDVSDLKRAQAAFEAALAERDLVLDALPLGVAFVENRVIARCNARLE
                     RMLGYEPGELAGQSARLLYPSDAAWRGGAAMYEALSGGALYEGDGELRRKDGTTQWCR
                     AWARTLNPAAPQEATIIAFSDIAERRAAEQALAASEALYRSLVETSNDLVWSVDRDGR
                     WTYLNPEAVRRIYGGRPADLLGHAFHEPVVPALRERDRAVFERVLAGEPVFRHETRHR
                     RSDGTSVDLSFNAVPLRDARGAIVGATGTARDITEERRAAAALHEGIEKLRLAVEAAE
                     LVYWEWDQASGELHWGRDPSGRLGAQNGHTVRWSEYVQLVHPEDRERYLAETRAAWSS
                     RQPYGAEYRVIGREGQVAWISARGKVLTDSAGRAQRMIGVSQDITERKRREEEDRFLA
                     YHDTLTGLPNRRLLDDRLRQALYLAQRRGTRLAVMLVDLDDFKRVNDERGHRAGDAVL
                     RQVAQRLADCVRKADTLARQGGDEFVVVIPELAQDGDAEVVAQKILRALAEPVRAEGE
                     ALRVGASIGISLHPGDAGDGDALLRNADAAMYRAKQSGGNQYCYYGR"
     gene            complement(7521..9563)
                     /locus_tag="AC16O9_08825"
     CDS             complement(7521..9563)
                     /locus_tag="AC16O9_08825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6516758.1"
                     /translation="MRRMLVRAACPHDCPDTCGMLVTVEDGVATKIQGDPAMPFTQGT
                     LCTKVAHYLERTYAPERLLHPLRRVGPKGSGRFERISWDAALDEIGARLKALAAEDPE
                     TILPLSYAGTMGMVQYSSMDRRFFHRLGASILDRTLCSSAGKAGIKATLGGSVGMDPE
                     RFDEAQLILLWGANPVVSNLHLWSRVQEAKRRGARVVAIDPYRSLSAEKCTQHVALLP
                     GTDGALALAMMHVLIAEDLLDRDYIARHTLGFDQLAERVRQYTPQWAAGVCGLAVEEI
                     VQLARAYGTTRPAAIRLNYGMQRHAGGGIAARTIACLPALTGAWRDVAGGIVLTTADF
                     YGFDHAALERPDLLGDRKPRVINHAAIGDALTAAEPPVKAVVVYNNNPVAVCPDSEKV
                     LAGFRREDLFCVVMDSFMTDTADYADLVLPATTQLEHYDVHKSYGHLYAVANNPAIAP
                     VGESLPNSEVFRRLAARLGFAEPCFRDSDEDICRTALASTQPRMRGIDWEQMKQRGWQ
                     RLDVPARFAPFAEGGFPTPSGKCEFYSAALQGQGIDPLPFYNPPAEPAVPGELQFISP
                     PARNFLNSSFAHLKRFRELEGEPRLEMHTTDAAARGIRDGDTVRVFNARGSLRLRARV
                     NDRPRPGVVVAPSVWWKKHSPDRGNANNLTGQRVADLGGGATFYDCRVQVERATHA"
     gene            complement(9565..10770)
                     /gene="glcF"
                     /locus_tag="AC16O9_08830"
     CDS             complement(9565..10770)
                     /gene="glcF"
                     /locus_tag="AC16O9_08830"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR6516759.1"
                     /translation="MQTELAAFIRDTPEGQEADAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLVKQVLEGATPTARTQLHLDRCLTCRSCETTCPSGVKYGRLADIGRGIV
                     EARVPRSLWQRVQRAALAFALPRRALFALLLGLGRPFRFLLPRLVPAPAAPAGAWPAP
                     RHARRMLTLAGCVQPALAPRINAAAARVLDRIGVSLVEVPGAGCCGALRYHLNDQDAG
                     CGDMRALIDAWWPVVERGEVEAIVVTASGCGVTVKDYGHLLAHDPAYAEKAARVAALA
                     RDLSEVIPLEAVPERAGLGRIAFHSPCSLQHGQQLRGGAERLLTRAGFELTPVPEAHL
                     CCGSAGTYSILQSELAEQLRGRKLAALQSGQPVGIVTANIGCLVHLQAGAQVPVAHWV
                     ELLDEALQA"
     gene            10794..11405
                     /locus_tag="AC16O9_08835"
     CDS             10794..11405
                     /locus_tag="AC16O9_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516760.1"
                     /translation="MQTFVILAALAVLVGVVVWVVIVMRKFEARKAFEREREAQFVAE
                     MLRAKPQAANPPAAKPAAPAAPATPAAPADPIAAAAEAIRAALAAGRGTDAAKAFLAV
                     VAERTKLTLEPAAWEGLGRALLAQGTYMEAAWALHAGAAIAGDAALAQKRLVEVAVRA
                     GDAGQAQHALKLYATLLAKYPQSQYADFVRANMKEQEKKVPKA"
     gene            complement(11402..12457)
                     /gene="glcE"
                     /locus_tag="AC16O9_08840"
     CDS             complement(11402..12457)
                     /gene="glcE"
                     /locus_tag="AC16O9_08840"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HIR6516761.1"
                     /translation="MAAHLRERIREAAARGTPLCLRGAGSKDFYGGAPRGEVLDTRAH
                     AGIVDYEPTELVVTARCGTPLAELERALAAQGQMLAFEPPHFGPGATLGGCVAAGLSG
                     PRRAAAGALRDYVLGVRLVDGRARELSFGGRVMKNVAGYDVSRLLAGSLGTLGLILEV
                     SLKVLPRPAAEHTLCLELPLEKALESLNRWAGTPLPVSASAWRDGELWVRLSGAETAV
                     RAAVVKVGGETLAAADAGRFWAGIREQTDPWFGGDAPLWRLSLPSAAPPLGLAGEGLI
                     EWGGALRWLRSRADAASVREATVRAGGHATLFRGGDKAAGVFAPLAPALARLHRELKA
                     AFDPAGVFNPGRLYPEL"
     gene            complement(12457..13944)
                     /locus_tag="AC16O9_08845"
     CDS             complement(12457..13944)
                     /locus_tag="AC16O9_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6516762.1"
                     /translation="MGEALPVAGRERQRAVARALGAFLPERAVLWQEEDTRPYECDGL
                     TAYRRVPMVVALPETEAQVQEVLRTCHALKVPVVPRGAGTGLSGGALPPGDGVLLSLA
                     KFMRIVRVDPVARVAVVQPGVRNLAISDAAASYGLYYAPDPSSQIACTIGGNVAENSG
                     GVHCLKYGLTVHNVLRVRGFAADGEPLDFGSEALDAPGLDLLALIVGSEGMLAVATEI
                     TVKLLPKPPAARVVMASFDDLQKAGDAVAAVIAAGIIPAGLEMMDRPATRAVEEFVGA
                     GYDLQAAAILLAEADGLPEEVDEDIAKLSEVLTRAGATRLTVSRDEAERLRFWSGRKA
                     AFPAMGRISPDYYCMDGTIPRKRLGEVLGFIGEMEKKYGLRCPNVFHAGDGNLHPLIL
                     FDANDPGQLKRTEEFAAEVLEKCVAVGGTITGEHGVGVEKINEMCAQFRDEELELFHA
                     VKRAYDPAGLLNPGKAVPTLARCAEFGHRHVHAATAAAHPGLPRF"
     gene            complement(13923..14702)
                     /gene="otnI"
                     /locus_tag="AC16O9_08850"
     CDS             complement(13923..14702)
                     /gene="otnI"
                     /locus_tag="AC16O9_08850"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HIR6516763.1"
                     /translation="MPRLAANVSLMFPEVPFMERFAAAARAGFRYVEYQFPYAFGGAA
                     EVAQRARDAGVEVVLHNLPGGDAAKGDRGIACQPARVAEFREGVERAIEYAKAAGCPR
                     LNALAGVAPADAAPELLFETLVENLRFAAGKLAAAGLTLLTEPANRRTIPDFFLCGSR
                     QGIEVIDAAGAPNLKLQYDIFHMQIVEGDLAKTIERLLPRIGHMQLADVPDRHEPGTG
                     EINFDFLLAHIDRLGYQGWMGCEYIPKGDTVEGLGWAKRYL"
     gene            14754..16526
                     /locus_tag="AC16O9_08855"
     CDS             14754..16526
                     /locus_tag="AC16O9_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6516764.1"
                     /translation="MPLPPAVARFLPFLAWWPRVTRATLRADASAALTSAIIVLPQGV
                     AFATLAGLPPEFGLYCAMVPAMVAAFFGSSWHAVSGPTNAISLYVFATVAPLAVPGSP
                     EYISLVLTLAFMSGALMLVMGAFGLGRLVNFVSHTVVVGFTAGAAVLIVASQLRNFFG
                     IDVPAGSSFLSTLRAFAAQAGAVQPWVMLVGALTLVAGAAARRYVPKAPYMIVALVAG
                     GLAGLVINLWLTPEVSGIRTIGLLPGALPGLTLPDLSADTLKSLMGIAVGITVLSLTE
                     AMSISRAVALKSGQRIDADQEFMGQGLANMSASVFSGYPVSASFNRSGLNYEAGAQTP
                     LAAALSAPLLAVVVFAVAPLLAYLPLASMAALLFMVAWGLLDFGAMRAIVRTSRSESA
                     VLGLTFAATLLMNLEAAILVGVTLSLIVYLNRTSRPQMRAVAPDPRHSVRRFGPVAQG
                     ITECPQLKIMAVEGSIYFGAVDHVESHFDTLRAVAREQKHLLVVARNINFLDIAGAEA
                     LVREARARRWRGGRLWLQGLRLPAEDVLRNGGYLAEIGTDAVFRDKREAIARIFDRLD
                     PGICARCRARIFEECPTRPLREDS"
     gene            16597..17607
                     /locus_tag="AC16O9_08860"
     CDS             16597..17607
                     /locus_tag="AC16O9_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6516765.1"
                     /translation="MNARITIAACAVAALASGTLHAQSTLDAVKSKGYVQCGVNTGLA
                     GFSQPDSRGVWKGIDVDTCRGVAAAVFGDATKVRYTPLTAQQRFTALQSGEIDVLARN
                     TTWTVSRDTSLGLNFVGVNYYDGQGFMVPKKLGVKSAKQLNGATICVQPGTTTELNLA
                     DYFRANKMSFKPVVIEKLEEVTNAYFSGRCDVFTTDVSGLVSTRASRAPNPADHVILP
                     EVISKEPLGPVVRHGDDRWFDVVKWTLFAMLEAEELGLSSKNIDSQVSSTNPSVQRFV
                     GATGDIGKMLGLDARWAYNVVKQVGNYGESFDANLTPLGFDRGINNLWTQGGLMYAPP
                     LR"
     gene            17630..18838
                     /locus_tag="AC16O9_08865"
     CDS             17630..18838
                     /locus_tag="AC16O9_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR6516766.1"
                     /translation="MAGDSRQGARAAAKRRASWSLNNPGVRALIYQVALVAAVLLGVW
                     YLASNTAHNLEVRKIASGFGFLGREAGFEIGESPFTAYGAADTYRKAFVVGLLNTFRV
                     ALIGIVLATLIGTLIGLARLSHNWLIAKISSGYIETVRNIPLLVQLFFWYAIISENLP
                     GPREALNPLPGVYLSNRGIVFPVFASHPVHVWMGVTLLAGIAAAWLVARWARARQART
                     GQIFPVFSTGAALILGLPLVAFLAAGAPTALNRPELAGFNFAGGGVLTPEFAALLAGL
                     TLYTAAFIAEIVRAGIIAVDRGQFEAAAALGLRPRRVMRLVILPQALRVIVPPMTSQY
                     LNLTKNSSLAVAIGYPDLVSIANTAMNQTGQAIEGIAVIMAVYLTISLSISAFMNWYN
                     ARIALKGARR"
     gene            18835..19929
                     /locus_tag="AC16O9_08870"
     CDS             18835..19929
                     /locus_tag="AC16O9_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR6516767.1"
                     /translation="MSARHAPAGASPLSWIIGNLFYSVPSALATLLLAYLAWKAIVPF
                     VDWAFVNAIWSAPDSKPCREARGAGACWALITEKHRFILFGTYPYEQHWRPALGILLL
                     IALYVVSAMRRFWRPALAGVWLAGLALIGVLMWGGVFGLPYVENERWGGLMLTLLLAT
                     IGIAFAFPLSIVLALGRRSDMPAVRVLCVGYIELIRGVPLISLLIMASVMLPLFLPEG
                     LSIDKLLRAQIAMILFAAAYLAEVVRGGLQAVPQGQVEAADAMGLSYWKKTLLIVLPQ
                     ALRVSIPPLVNTFIGFFKDTSLVVIIGLFDLLTSIKVSIAEPAWTGFGVEAYIFASLV
                     YFAFCYAMSQYSQNLERELARGRAHYLREN"
     gene            19932..20672
                     /locus_tag="AC16O9_08875"
     CDS             19932..20672
                     /locus_tag="AC16O9_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR6516768.1"
                     /translation="MAQPMIEIKGVNKWFGAFHVLKNVELSVAQGEKIVVCGPSGSGK
                     STLVRLINRLESHQEGRIVVDGIELAEDLKAIEAIRREVGMVFQSFNLFPHLTVLENL
                     TLAPIWVRRMPKKDAEELAMQLLKRVRIAEQAAKYPAQLSGGQQQRVAIARALAMRPK
                     VMLFDEPTSALDPEMIKEVLDVMIELAQEGMTMLVVTHEMGFARTVADRVVFMDQGEI
                     VESGAPKTFFEAPKTDRARLFLSQILGH"
     gene            20724..21320
                     /locus_tag="AC16O9_08880"
     CDS             20724..21320
                     /locus_tag="AC16O9_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR6516769.1"
                     /translation="MGAILSSLSRTIVAGIVVLVVILLIVGQATGAWINLGSHAWWAF
                     FMRWLHVLSGVMWIGLLWYFNFVQTPSMPKIPDEHKPAISKVIAPEALFWFRWAALAT
                     IVTGLLLATMSGWLVQALSLQRPFTAIGIGMWLGLVMAFNVWFIIWPNQKKALGMVQV
                     GAEEKAAAAKKAGMTSRLNTLLSIPMLYCMVAQQNGGL"
     gene            complement(21321..21755)
                     /locus_tag="AC16O9_08885"
     CDS             complement(21321..21755)
                     /locus_tag="AC16O9_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR6516770.1"
                     /translation="MKRLFLVLLLAAAGGHAQQPTLPTVQLGAGMHLIRAEVADHDAA
                     RAMGLMLRTSLAPNGGMLFVFDETSVHCMWMKNTLLPLSVAFIDERGAIVNIADMQPQ
                     TEDSHCAARPARFALEMTQGWFAARGIRAGFRLRGLDQLKPR"
     gene            complement(21810..21995)
                     /locus_tag="AC16O9_08890"
     CDS             complement(21810..21995)
                     /locus_tag="AC16O9_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516771.1"
                     /translation="MNKLLTVIAAAMFAAVSASAFAASHAGAPMKEGEKKVEKKADKK
                     KAAKKKEAKEAKEGDKK"
     gene            22228..23322
                     /locus_tag="AC16O9_08895"
     CDS             22228..23322
                     /locus_tag="AC16O9_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516772.1"
                     /translation="MPSDDAEILERLSLVRDSPESAALRQLRARVAAAPTDPAPAAAL
                     ARRYFGLAMAQGDPRFVGYAQAVLRPWDEALAPADILLVRAMLRQYRHDFDGALRALA
                     LALERDRNNAEGRAWRAAIFMVRAEYGKAAGECAALAALSDDLLAAGCSAYVEATTGR
                     TRAAYDRLRAALEQHADADMGVRLWVQTRLAEMADRLGDAAAAENHFRAALALGIDDN
                     FLLAAYADFLLEQGRQREVVALLEDRGRSDTLLLRLALAMHELGMPEAAKQARVLAER
                     FAAAALRGDRLHLAEEARYLLELRRDAGAALAAAAENWKSQREPRDALILLRAARAAR
                     APAAAAPVLDWLARTGFEHDGMRRLAAELR"
     gene            23319..24440
                     /locus_tag="AC16O9_08900"
     CDS             23319..24440
                     /locus_tag="AC16O9_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6516773.1"
                     /translation="MKAVVLFLALAFAGGPALAHKPSDSYLRVRVEGALLRGQWDIAL
                     RDLEYAIGLDADGDGAITWGEVRAREADIAAYAFARLALSADGQTCGAESRRLLVDEH
                     SDGAYAVLEFEARCAAAPQRTLRFDYSLFADLDPTHRGLLQVQQGAATASGVLGPDHP
                     RLELAVGTVSRLRQFLDYAREGVWHIWIGIDHALFLVSLLLPAVLVYGARAWEPAPRF
                     APAFWDVFRIVTAFTVAHSITLSLAALGVVALPSRLVESVIALSVMLAALNNLRPWVL
                     GRRWAVAFGFGLVHGFGFASVLADLGLPREALLVSLVGFNVGVEAGQLAIVGAFLPAA
                     FLARRRWAYRRLAFAGGSVAIALLAAAWLVERAFDLKLL"
     gene            24476..25732
                     /gene="icd"
                     /locus_tag="AC16O9_08905"
     CDS             24476..25732
                     /gene="icd"
                     /locus_tag="AC16O9_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR6516774.1"
                     /translation="MAYKHIKVPATGEKIKVNKDFSLAVPDTPVIPYIEGDGTGVDIT
                     PVMIKVVDAAVKKAYGAKKKIVWMEMYAGEKSCKVYGENVWLPDETLAASREYVVSIK
                     GPLTTPVGGGIRSINVALRQELDLYVCLRPIRYFKGVPSPLKEPEKTDMVIFRENSED
                     IYAGIEWAAKTPEVQKLIKYLTDEMKVKKIRFPETSAIGIKPVSVQGSERLIRKAFQY
                     AVDNDRKSVTLVHKGNIQKFTEGAFRDWGYALAQKEFGAKLVDGGPWCSFKNPKTGRE
                     IVVKDVITDAFLQQILLRPAEYDVIATMNLNGDYVSDALAAQVGGIGIAPGANMSDSI
                     AMFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADVIVRSMEKAIADKVVT
                     YDFARLMQGAKQVSCSGFGQAMIERM"
     gene            complement(25802..26005)
                     /locus_tag="AC16O9_08910"
     CDS             complement(25802..26005)
                     /locus_tag="AC16O9_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6516775.1"
                     /translation="MATGTVKWFNDAKGYGFITPDDGSEDLFAHFSAIQMAGFKTLKE
                     GMKVSFDVVQGPKGKQASNINKV"
     gene            26318..26623
                     /gene="clpS"
                     /locus_tag="AC16O9_08915"
     CDS             26318..26623
                     /gene="clpS"
                     /locus_tag="AC16O9_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR6516776.1"
                     /translation="MAQFKDDILLEAQRSKLQPPPMFKVMLLNDDYTPMEFVVMVLQK
                     FFGMTRERATQVMLKVHREGIGVCGVYPQDIASTKVQQVAGYARRHQHPLQCVMEET"
     gene            26625..>27159
                     /locus_tag="AC16O9_08920"
     CDS             26625..>27159
                     /locus_tag="AC16O9_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR6516777.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPTAAEVL
                     RACGANLDELRKSLTQHVAEQTPRIAADREVDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLQQQGVTRLDVVNYISHGITKTPQPHAGKAEQEAGDEA
                     QAEPSNSPLDNYTQNLNA"
BASE COUNT         3998 a         9655 c         9404 g         4102 t
ORIGIN      
        1 gagacggttg ccagcgttgc ggacatgagt cgtctcccct atttccggaa catctgcagc
       61 atgcgccgcg tgacggcgaa accgccgaac atgttgatgg cggtgaggcc cacgccaacg
      121 accgcgagcc agcggatcca ctcctccggc cgggcggccc ctgcaccggc cgcgagcggc
      181 ggcgcgatct ggaccagcgc gccgatcgcg atgatgctgc tgatcgcgtt ggtgacgctc
      241 atcagcggcg tgtgcagcgc cggggtgacg ttccacacca ccatgtagcc cacgaagcaa
      301 gcgagcacga acaccgtgaa gtggttgagg aacgacgccg gcgcattggc gccgatgaac
      361 cagaacagca gcgcggccac cacgaacgtg atcgccatcg ccctgcccga gcgcggcgcg
      421 ccggcgccgt ggccgtgccc cttgggcgcg ggcggcgcgg ccgccggctt ggcggcggcc
      481 ggcggcgccg gcagcttggg cggcggcggc ggccaggtga tgctgccctg cttgatgacc
      541 gtcaggccgc ggatggcgtc gtcttccatg ttgacgtcga tgacgccgtc cttgcccttg
      601 cacagctcct cggcaaggcg gaacaggttg gtcgcataca gcgtcgagga ctgtttggcg
      661 agccggctgg gcaggtccgt atagccgatg atcgtgacgc cgtgcttcac cacggccttg
      721 ccggcctcgg tcagctcgca gttgccgccc tgctccgcag ccatgtcgac gatgacgctg
      781 cccggcttca tgctacgcac catgtcggcc gtgatcagcc gcggcgcggg cttgcccgga
      841 atcagcgcgg tggtgatgat gatgtccacc tccctcgcct gcttcgcgta catctcgcgc
      901 tgggcctgct ggaagccttc gctcatcacc ttggcgtagc cgccaccgcc ggcgccttct
      961 tcctcgtagt cgaccttcac gaactcgccg cccatcgaca ccacctggtc agccacctcg
     1021 gcccgggtgt cgttggcgcg cacgatggcg cccaacccga cggcggtgcc gatcgcggac
     1081 aggccggcca cgccggcgcc ggcgatgaac accttggccg gaggaatctt tccggcggcg
     1141 gtcacctggc cgttgaagaa gcggccgaag gcgttggcgg cctcgacgac ggcgcggtag
     1201 ccggagatgc tggccatgga ggtcaacgcg tccatcttct gggcgcggct cagcatgcgc
     1261 ggcagcgagt cgatcgccag cacggtgacc ccgcgcgccg cgaggctctg catcagctcg
     1321 gagttctgcg ccggccagat gaaagagacc agcgtctggc tcccgcgcat cagaccgacc
     1381 tcttcagccg tgggcgcacg caccttgaag acgatgtccg ccgcggacca gagggccttg
     1441 gcgtccggcg cgatttgcgc gcctgccgcc tcgtaggacg catcgtcgaa gttcgcgccg
     1501 gcgcccgcgc ccgattgcac catcaccttg aagccgagct tgatcagctt ttgcgcgacc
     1561 tcgggcaccg tcgccacgcg cttttcgtcg gcagccgact cccttggcac tccaatcacc
     1621 tgcgacatgg atctcccttt tgaatagccg gaccggattt cgctcgaggc cgctgccccg
     1681 ggtgcccaag tctactttcg cggccgggtg cgggcttgat ctgcatcata ccttcccctg
     1741 ccccggggct tcgcgcatcc atgcgcaacg gcgctcggcg agctcgaacg ccccctgcac
     1801 cagcagcgcc agcgccgcgg cgggaatcgc gccggccagc agcatgacgt tgtcgttcaa
     1861 cgccaggccc gaggcgatgc gctcgccgta gccgccggcg ccgatgaacg ccgcgatggt
     1921 ggcggtgccg acgttgatca ccgcggaggt cttgacgccg gcgaggatcg tgggcagcgc
     1981 gagcggcagc tcgattctcc acagccgcac gcggcgtggc agcgcgagcg cgagcgccgc
     2041 ctgatgcatg cctgcggcga tgccggcgag cccggcgtgg gtgttgcgca cgatcggcag
     2101 cagtgcatac aggaacagcg ccagcagcgc cggccaggtg ccgatggcgc cggtgagcgc
     2161 gatgaggaaa gcgaacagcg cgagcgacgg gatggtctgg atcacgccca ccacgccgag
     2221 gatcggctgc gccaggcgcg gcagcttcgc ggccgcgacg ccgagcggca cggccgcggc
     2281 gacgctcgcg gcgagcgagg cgaacaccag cagcaggtgc tcgcgcgtca ggcgccagaa
     2341 atccgggccg aacagcgcgg ccatcagggt gcgcgcgccg ggccgcggcg gggccgcgcg
     2401 ccccgcgccg tgcaggcggg cggcgtcggc gaagctcttg ccctcgagct cggcggcggc
     2461 gttcatgcgc cgcatgcgct cggcgtcgat cgtgccctcg agcccgcgca gtgcgcgcca
     2521 ggcgcgcgga aaacgctccg gcacgccggt gcgatgcagc agcaccgcgt cgtaggcggg
     2581 aaagaagccg cggtcgtcct cgagaatcgc gatggcgtag cgcgcgatct tggcgtcggt
     2641 cgaatacagg tcgatgacgt ccacctcgcc cgcggcgagc gcctcgtagg caagcccgtg
     2701 atccaggccg cgcgggcgct gcgccaggcc gtaggcggcg gcgagccccg gccagccgtc
     2761 gcgccgggcg aggaactcgt ggccgaagcc gaaacgcagc cgccgctgcc ccttcaggtc
     2821 ggagatgcgc ccgagccccc ggcgctctgc cagcgccgcg cgcacgccga tggcgtaggt
     2881 gttgttgaag ccgagcggca ccgacgccgc caggccgagc ggtgcgaggc gcgcattcag
     2941 cgcaccgagc gaggcgtgcg tctcgccctg caggatctcg cgcgcgatgg tgccggtgta
     3001 ctccggatac aggtcgatgg cgccggtgcg cagcgcctcg tacaggatct tggtgccgcc
     3061 catgccgggg cgcagctcga cctcggcgcc ggcggcgcgc gcggtctgcg cgatgagctc
     3121 gccgaggagg taggactcgg tgaagcgctt ggagcccacc accagcggct ccgcctgcgt
     3181 cgccgcggcc gcggccgcga gcgccagcag caccgccgct cgcctcatgc ggcgcgcgcg
     3241 accctcgccg ccgcgccgtc gagaaactgc aactgcgcga ggctcgcgta cagcccgccc
     3301 tggcgcatca gctcggcgtg cgtgccctgg gcgacgatcg tgccgcggtc catcaccacg
     3361 atgcggtcgg catgctgcac ggtggccagg cgaggcgcga tcaccagcgt ggtgcgcccg
     3421 tgctccaggc gctcgagcgc ctgctgcacc atgcgctcgc tctcggcgtc gagcgagctg
     3481 gtcgcttcgt cgagcagcag cagcggccgg tcggcgagca gcgcacgcgc gatcgacagg
     3541 cgctggcgct ggccgcccga gagcgtgacg ccgcgctcgc cgagcggcgt gtcgaggccc
     3601 tgcggcagcc gcgcgatgaa ctccgcggcg aacgcctgct cgcaggcgcg ctcgacctcg
     3661 gcccggctcg cctccggccg gccgtagcgc acgttgtcga aggcgctcgc cgcgaagatc
     3721 accggctcct gcggcaccac cgccatcagg ccgcgcaggg cgcgcggctc catgtcgcgc
     3781 aggtccacgc cgtcgacgcg gatcgcaccg ccttgcgggt cgtagaaccg gagcagcagc
     3841 gcgagcaccg tgctcttgcc ggcgccggag gggccgacca gcgcgacgcg ctcgccggcg
     3901 gcaaggcgca ggctgaagcg ctccagcgcg gcggcgtgcg gccggccggc gtaggcgaag
     3961 ctgaccgcat cgaactcgta cacgcgcggc gcggccggca ccggacgcgg cgcggccggc
     4021 gcggcgatcg cgggccgcgc atcgagcagc tcgaacaggc gctcggtggc gccggcggcg
     4081 cgctgcaggt cgccccacac ctcggacacg gtgcccgcgc cggtggccac cacgccggcg
     4141 tagaacacga aggccgcgag ctcgcccgcg ctcaggcgac cggcgagcac gtcgtggccc
     4201 cccacccaca ggatgacgcc caccgcgcag aacgcgatca gcatcaccgt cgcgatgagc
     4261 gcggacttct gccccaggct ggcggcggcg gcggcatacg cggcctcggc atgctgcgcg
     4321 aacgccgcgc gatccgtgtc ctcgtgcgcg taggcctgca ccgtgcgcac ctcgtgcacg
     4381 gcctcgtcga cgtgcgccga gacgtcggcg acgcggtcct ggctgcggcg cgacaggcgc
     4441 cgcacgcggc ggccgagcag caggatcggc accagcgtcg ccggcacgcc ggcgagcacc
     4501 agcgccgcca gcttccagct ggtgaagaac agcatcgccg ccgcgccgac catcatgagg
     4561 aggttccgca cgaacatcga caggccgtag ccgatcacct gctgcaccac ggtggtgtcg
     4621 ctcgccaggc gcgacaccaa ctcgccggtg cgcgccgtct cgtagaaccc cggcgagagc
     4681 ccgagcaggt gatcgaacac cgcccggcgc aggtcggtga ccacgcgctc gccggtgctc
     4741 atcatcaggt agaagcggaa gtaggtggcg atcgcgagcg tcacggccac cgccaccgcg
     4801 gcggcgagcg cctggtcaag caggcgcggc tcgccgctgc cgaagccggc gtcgaccacg
     4861 tacttcaggc cctgccccag cgccagcacg caggccgccg ccgccaggag ggcggcgagc
     4921 gccgcggcga ctcgccagcg gtacggggcg agaaagcgcg ccaggcgcgc gagcgaaccc
     4981 atgcgcgcaa ctctaccgca gcgcgctagc ggccgtaata gcagtactgg ttgccgcccg
     5041 attgcttcgc gcggtacatg gcggcgtcgg cgttgcgcag cagcgcgtcg ccgtcgccgg
     5101 cgtcgcccgg gtgcagcgag atgccgatac tcgcgccgac gcgcagcgct tcgccttcgg
     5161 cgcgcaccgg ctcggcgagc gcgcgcagga tcttctgcgc cacgacctcg gcgtcgccgt
     5221 cctgcgcgag ctcggggatc accaccacga actcgtcacc gccctggcgc gcgagcgtat
     5281 cggccttgcg cacgcagtcc gcgagccgct gcgccacctg gcgcagcacc gcgtcgcccg
     5341 cgcggtgtcc ccgctcgtcg ttcacgcgct tgaagtcgtc gagatcgacg agcatcacgg
     5401 cgaggcgcgt gccgcgccgc tgcgcgaggt acagcgcctg gcgcaggcgg tcgtccagca
     5461 agcggcggtt gggcagcccc gtgagcgtgt cgtggtaggc gaggaagcgg tcttcctcct
     5521 cgcggcgctt gcgctcggtg atgtcctgcg acaccccgat catgcgctgc gcgcggcccg
     5581 cgctgtcggt gagcaccttg ccgcgcgccg agatccatgc cacctgcccc tcgcggccga
     5641 tcacgcggta ctccgcgccg tacggctgcc gactgctcca ggcggcacgc gtttcggcga
     5701 ggtagcgctc gcgatcctcc ggatgcacca gctgcacgta ctcgctccag cgcacggtat
     5761 gcccgttttg cgcgccgagg cgccccgacg ggtcccggcc ccaatgcagc tcgccgctgg
     5821 cctggtccca ctcccagtag accagctccg ccgcctcgac ggcgaggcgc agcttctcga
     5881 tgccctcgtg cagcgcggcg gccgcgcgcc gctcctcggt gatgtcgcgc gccgtgccgg
     5941 tggcgccgac gatcgcgccg cgcgcgtcgc gcagcggcac cgcgttgaac gacaagtcga
     6001 cgctggtgcc gtcgctgcgt cggtggcgcg tctcgtgccg gaataccggc tcgcccgcga
     6061 gcacccgctc gaacaccgcg cggtcgcgct cgcgcagcgc cggcaccacc ggctcgtgga
     6121 acgcgtgccc gaggagatcg gcagggcggc cgccgtagat ccggcgcacc gcttccggat
     6181 tgagataagt ccagcggccg tcgcggtcga ccgaccacac caggtcgttc gaggtctcga
     6241 ccaggctgcg gtacagcgcc tcgctcgcgg cgagcgcctg ctcggccgcc cgccgctcgg
     6301 cgatgtcgga gaacgcgatg atcgtcgcct cctgcggcgc cgcggggttc agcgtgcgcg
     6361 cccaggcgcg gcaccactgc gtcgtgccgt ccttgcgccg cagctcgccg tcgccctcgt
     6421 agagcgcgcc gccgcttagg gcctcgtaca tcgccgcgcc gccgcgccag gctgcgtcgc
     6481 tcggatagag caggcgcgcg gactgtccgg cgagctcgcc cggctcgtag ccgagcatgc
     6541 gttcgaggcg tgcattgcag cgcgcgatga cgcgattctc gacgaaagcg acgcccagcg
     6601 gcagcgcatc gagcaccagg tcgcgctcgg cgagcgcggc ctcgaacgcc gcctgcgcgc
     6661 gcttcaggtc gctcacgtcg gtgaacacgc ccaccgcgcc cccgcgctcc atgcgcacga
     6721 agtggccctg catccagcgt ccatcgcggg tgcggaactg gcgcacggcg ggcgcctccg
     6781 ccgcgcccgc cgcgcgcgcc tcgcccgcga ccgtcgtgta agcatcgttg gacgcgagca
     6841 cgcgcccgtc ctcgtcgaac accgcgatgc cctcggggaa gccctcgatc gcgtcgcgca
     6901 gccgcgcctc gctctgtgcc acgcgctcga gcagcgcctc cttctcggca cgcgcgccga
     6961 gcgcgcccac cacgttggcg tgcatgtcgc ggtacacgcg caccatggcc gcgacgaaca
     7021 ccagcgcggc cagcgcgagc agcggataga cctcgccggg ctggcgcgcg aactggacga
     7081 cgacggcgag gatcgggggc aggcagaaac cgtagaacgc ggccgcgctg gcggcgtaca
     7141 cccccgccgc gccgaccacg gcggccgccg tcacgagcaa cacggccgtc tgcagcaccg
     7201 gcgcctgcgg gaagaacgcg gcgatcggca gcgcccacag cagcccggcg gtgaacgccc
     7261 cgagcgcaaa gcgccgctcc caggcgagcg gcgtgcgccg ctccgggtgc gcggcgtggg
     7321 cgcgatgcag cccgaggcgc gccagcgtca ccgccgccag cacgccgtac caggtggcga
     7381 gcgctgcgac gggcgtctcg ccccagagca tcgccaccac caaagcgccc gcaaccagcg
     7441 tcgccgcata caccgtcttg gcgatgccga acagcgccgc gacccgcgcc gcgtcaacgc
     7501 gggcctgcgg caggatcaga tcaagcgtgc gtggcgcgtt caacctgcac ccggcagtcg
     7561 tagaacgtgg cgccgccgcc caggtcggcg acgcgctgcc cggtcaggtt gttggcatta
     7621 ccgcggtcgg gcgagtgctt cttccaccac accgagggcg cgaccaccac gccgggacgt
     7681 ggccggtcgt tcacgcgcgc gcgcagtcgc aggctgccgc gcgcgttgaa cacacgcacg
     7741 gtgtcgccgt cgcggatgcc gcgcgccgcg gcgtccgtgg tgtgcatctc taggcgcggc
     7801 tcaccctcaa gctcgcggaa gcgcttcagg tgcgcgaagc tcgagttcag aaagtttcgc
     7861 gccggcggcg agatgaactg cagctcgccc ggcaccgccg gctcggcggg cgggttgtag
     7921 aacggcagcg ggtcgatgcc ctgcccttgc agcgccgcgc tgtagaactc acacttgccc
     7981 gagggcgttg ggaagccgcc ttcggcgaac ggcgcgaagc gcgccggcac gtcgaggcgc
     8041 tgccagccac gctgcttcat ctgctcccag tcgatgccgc gcatgcgcgg ctgcgtactg
     8101 gcgagcgccg tgcggcagat gtcctcgtcg ctgtcgcgaa agcacggctc cgcgaacccc
     8161 aggcgcgcgg caaggcgccg gaacacttcg gaattcggca atgactcgcc caccggcgcg
     8221 atcgcggggt tgttggccac cgcgtacagg tgcccgtagg acttgtgcac gtcgtagtgc
     8281 tcgagctgcg tggtcgcggg cagcaccagg tcggcgtagt ccgcggtgtc ggtcatgaac
     8341 gagtccatca ccacgcagaa gaggtcctcg cggcgaaagc ccgccagcac cttctccgag
     8401 tccgggcaca cggccaccgg gttgttgttg tagacgacca ccgccttcac cggcggctcg
     8461 gccgcggtga gcgcgtcgcc gatcgccgcg tggttgatga cgcggggctt cctgtcgccg
     8521 agcagatcgg gccgctcgag cgccgcgtgg tcgaagccgt agaagtccgc cgtcgtgagc
     8581 acgatgccgc cggcgacgtc gcgccaggcg ccggtcagcg ccggcagaca ggcaatggtg
     8641 cgcgcggcga tgccgccgcc ggcgtggcgc tgcatgccgt aattcagccg gatggcggcg
     8701 ggccgcgtcg tgccgtacgc ccgcgcaagc tgcacgatct cctcgacggc gaggccacag
     8761 acgcccgcgg cccactgcgg cgtgtactgc ctgacgcgct cggccaactg gtcgaatccg
     8821 agcgtgtggc gcgcgatgta gtcgcgatcg agcaggtctt cggcgatcag cacgtgcatc
     8881 atggcgagcg cgagcgcgcc gtcggtgccg ggcagcagcg ccacgtgctg cgtgcatttc
     8941 tcggccgaca ggctcctgta gggatcgatc gccaccaccc gggcgccgcg gcgcttggcc
     9001 tcctgcacgc gcgaccacag gtgcaggttg gacaccacgg gatttgcgcc ccagagcagg
     9061 atcagctgcg cctcgtcgaa gcgctcgggg tccatgccga ccgagccgcc aagcgtggcc
     9121 ttgatgcccg ccttgccggc cgaagagcac agcgtgcggt cgaggatcga ggcgccgagc
     9181 cgatgaaaga agcgccggtc catcgagctg tactgcacca tgcccatggt gccggcgtag
     9241 ctgagcggca ggatcgtttc cgggtcctct gcggcgagcg ccttgaggcg cgcgccgatc
     9301 tcgtcgagcg ccgcgtccca ggagatgcgc tcgaagcgcc cgctgccctt ggggccgacg
     9361 cgccgcaagg ggtgcagcag ccgctcgggc gcgtaggtgc gctcgaggta gtgcgcgacc
     9421 ttggtgcaga gcgtgccctg cgtgaacggc atcgcgggat cgccctggat cttcgtggcg
     9481 acgccgtctt ccacggtcac cagcatgccg caggtatcgg ggcagtcgtg cgggcaggcg
     9541 gcgcggacca gcatgcgccg gattttacgc ttgcagggct tcgtccagca gttccaccca
     9601 gtgcgcgacc ggcacctgcg cgcccgcctg caggtgcacc aggcaaccga tgttcgcggt
     9661 gacgatgccg accggctgcc cgctctgcag cgccgcgagc ttgcggccgc gcagctgctc
     9721 cgcgagctcg gattgcagga tcgaataggt gcccgccgag ccgcagcaca ggtgcgcctc
     9781 ggggaccggc gtgagctcga agccggcgcg cgtgagcagc cgctcggcgc cgccgcgcag
     9841 ctgctggccg tgctgcaacg agcaagggga atggaacgcg atgcgcccaa ggccggcgcg
     9901 ctcgggaacc gcctcgagcg ggatcacctc gctcaggtcg cgcgccagcg ccgcgacgcg
     9961 cgccgctttt tccgcgtagg cgggatcatg ggcgagcaga tggccgtagt ccttcacggt
    10021 cacgccgcag ccgctcgccg tcaccacgat cgcctccact tcgccgcgct cgacgaccgg
    10081 ccaccaggcg tcgatcagcg cgcgcatgtc gccgcagccc gcgtcctggt cgttgaggtg
    10141 atagcgcagc gcgccgcagc agcccgcgcc cggcacctcg acgagcgaca cgccgatgcg
    10201 atcgagcacg cgcgcggcgg ctgcgttgat gcgtggcgcg agcgccggct gcacgcagcc
    10261 cgcgagcgtg agcatccggc gcgcatgccg tggcgccggc caggcgccgg cgggcgccgc
    10321 cggcgcgggc acgaggcgcg gcaacaggaa ccggaacggg cggcccagcc ccagcagcag
    10381 cgcgaacagc gcgcgccgcg gcagggcgaa ggcgagcgcg gcgcgctgta cccgctgcca
    10441 gagcgagcgc ggcaccctgg cctcgacgat gccgcggccg atgtcggcga gccgcccgta
    10501 cttcacgccc gacgggcagg tggtctcgca cgagcggcag gtgaggcagc ggtccaggtg
    10561 cagctgcgtg cgcgccgtcg gcgtagcgcc ttccagcacc tgcttgacga ggtagatgcg
    10621 cccgcgcggc ccgtcgagct cgtcgccgag cagctggtag gtcgggcagg tggccgtgca
    10681 gaacccgcaa tgcacgcact tgcgcaggat ggcgtcggcc tcctgtccct cgggcgtgtc
    10741 gcggatgaag gcggcgagtt cggtctgcat cggcgggccg ctatactcgt tgcatgcaga
    10801 cgttcgtcat cctcgccgcc ctcgccgtgc tcgtcggcgt cgtggtgtgg gtcgtcatcg
    10861 tcatgcgcaa gttcgaggca cgcaaggctt tcgagcgcga gcgcgaggcg cagttcgtcg
    10921 ccgagatgct gcgtgcaaaa ccgcaagccg cgaatccgcc ggcggcaaag ccggccgcgc
    10981 ctgcggcacc cgccacgccc gccgcaccgg ccgatccgat cgccgccgcg gcggaagcaa
    11041 tccgcgcggc gctcgccgcc ggccgtggca cagacgcggc gaaagcgttc ctcgcggtcg
    11101 tcgccgagcg taccaagctc acgctcgagc cggcggcctg ggagggcctc ggccgcgcgc
    11161 tgctcgcgca gggtacgtat atggaggccg cctgggcgct gcacgccggc gccgcgattg
    11221 ccggcgacgc tgcgctcgcg cagaagcgcc tggtcgaggt cgcggtgcgc gccggcgacg
    11281 ccggccaggc gcagcacgcg ctcaagctgt acgcgacgct gctcgccaag tacccgcagt
    11341 cgcagtacgc cgatttcgtg cgcgccaaca tgaaggaaca ggaaaagaag gtgcccaagg
    11401 cctagagctc cgggtagagc cgtccagggt tgaacacgcc tgccgggtcg aacgccgctt
    11461 tcagctcgcg gtgcaggcgc gccagcgccg gcgcgagcgg cgcgaacacc cccgccgcct
    11521 tgtcgccacc gcgaaacagc gtggcgtggc cgccggcgcg caccgtggct tcgcgcacgc
    11581 tggcggcgtc ggcgcgcgag cgcagccagc gcagcgcgcc gccccactcg atcagcccct
    11641 cgccggcgag cccgagcggc ggcgcggccg aaggtagcga caggcgccat agcggcgcgt
    11701 cgcccccaaa ccagggatcg gtctgctcgc ggatccccgc ccagaagcgc ccggcatccg
    11761 ccgccgcgag cgtctcgccg ccgaccttta caaccgctgc ccgaaccgcg gtctcggcgc
    11821 ccgagaggcg cacccagagc tcgccgtcgc gccaggcgct cgccgacacc ggcagcggcg
    11881 tgccggccca ccggttgagc gactcgagcg ccttttccag cggcagctcg aggcacagcg
    11941 tgtgctcggc cgccggccgc ggcagcacct tgagcgagac ctcgaggatc aggccgagcg
    12001 tgccgagcga gcccgcgagc aggcgcgaca cgtcgtagcc cgccacgttc ttcatcacgc
    12061 gcccgccgaa cgagagctcg cgcgcgcgcc cgtcgacaag gcgcacgccg agcacatagt
    12121 cgcgcagcgc cccggcggct gcgcggcgcg ggcccgagag gcccgcggcg acgcagccgc
    12181 caagggtcgc gccggggccg aagtgcggcg gctcgaaggc gagcatctgg ccctgggcgg
    12241 cgagcgcgcg ctcgagctcg gcgagcggcg tgccgcagcg cgccgtcacc accagctcgg
    12301 tcggctcgta gtcgacgatg ccggcatgcg cgcgcgtatc gagcacttcg ccgcgcggcg
    12361 cgccgccata gaagtccttg ctgcccgcgc cgcgcaggca cagcggcgtg ccgcgcgccg
    12421 ccgcttcgcg gatgcgctcg cgcaggtgcg cggccatcag aagcgcggca ggcccgggtg
    12481 cgccgcggcg gtggccgcgt gcacgtgccg gtggccgaac tcggcgcagc gcgcgagcgt
    12541 gggcaccgcc ttgccggggt tgagcaggcc cgcgggatcg taggcgcgct tcacggcgtg
    12601 gaacagctcg agctcctcgt cgcggaactg cgcgcacatt tcgttgatct tctccacgcc
    12661 cacgccgtgc tcgccggtga tggtgccgcc gaccgcgacg catttctcga gcacctcggc
    12721 cgcgaattcc tcggtgcgct tgagctggcc ggggtcgttg gcgtcgaaca ggatcagcgg
    12781 gtgcaggttg ccgtcgccgg cgtggaacac gttcgggcag cgcagcccgt acttcttctc
    12841 catctcgccg atgaagccga gcacctcgcc caggcgcttt ctcgggatgg tgccgtccat
    12901 gcagtagtag tcgggcgaga tgcggcccat cgccgggaac gccgccttgc gcccggacca
    12961 gaagcgcagc cgctcggcct cgtcgcgcga cacggtcagg cgcgtggcgc cggcgcgcgt
    13021 cagcacctcg ctcagcttcg cgatgtcctc gtccacttcc tccggcaggc cgtcggcctc
    13081 ggcgagcagg atcgccgccg cctgcaggtc gtagccggcg ccgacgaact cctcgaccgc
    13141 ccgcgtcgcc ggccggtcca tcatctccag gcccgcgggg atgatgcccg cggcgatcac
    13201 cgcggcgacc gcgtcgcccg ccttctgcag gtcgtcgaac gaggccatca ccacgcgcgc
    13261 cgcaggcggc ttaggcagca gcttcaccgt gatctcggtg gccaccgcca gcatgccctc
    13321 cgagcccacg atcagcgcca gcaggtcgag gcccggcgca tcgagcgctt ccgagccgaa
    13381 atcgagcggc tcgccgtcgg cggcgaagcc gcgcacccgg agcacgttgt gcaccgtgag
    13441 cccgtacttc aggcagtgca cgccgcccga attctccgcg acgttgccgc cgatggtgca
    13501 ggcgatctgg ctcgacggat cgggcgcgta gtacaggccg taggacgcgg ctgcgtccga
    13561 gatggcgagg ttcctcaccc ccggctgcac caccgcgacg cgcgccacgg gatcgacgcg
    13621 cacgatgcgc atgaacttgg cgagcgagag cagcacgccg tcgcccggcg gcagcgcgcc
    13681 gcccgaaagg ccggtgcccg cgccgcgcgg caccaccggc accttgagcg cgtggcaggt
    13741 gcgcagcact tcctgcacct gcgcctcggt ctccggcagc gccaccacca tcggcacgcg
    13801 ccggtacgcg gtgagcccgt cgcattcgta ggggcgcgtg tcctcttcct gccacagcac
    13861 cgcgcgctcc ggcaggaagg cgccgagcgc ccgcgccacg gcgcgctggc gctcgcggcc
    13921 cgctacaggt agcgcttcgc ccacccgagc ccctcgaccg tgtcgccctt cgggatgtac
    13981 tcgcagccca tccagccctg atagcccagg cggtcgatgt gcgcaagaag gaaatcgaag
    14041 ttgatctcgc cggtgccggg ctcgtgccga tcgggcacgt cggcgagctg catgtggccg
    14101 atccgcggca gcaggcgctc gatggtcttg gcgaggtcgc cttcgacgat ctgcatgtgg
    14161 aagatgtcgt actgcagctt caggttcggg gcgcccgcgg cgtcgatcac ctcgatgccc
    14221 tggcgcgagc cgcacaggaa gaaatccgga atcgtgcgtc ggttcgcagg ctcggtgagc
    14281 agcgtcaggc cagccgccgc gagcttgccg gcggcgaagc gcaggttctc caccagcgtc
    14341 tcgaacagca gctccggcgc ggcgtccgcc ggcgcaacgc cggcaagcgc gttcaggcgc
    14401 gggcagccgg cggccttcgc gtactcgatg gcacgctcca cgccctcgcg aaactcggcg
    14461 acgcgcgctg gctggcaggc gatgccgcga tcgcccttgg cggcgtcacc gcccggcagg
    14521 ttgtgcagca ccacttcgac gcccgcgtcg cgcgcgcgct gcgccacctc ggccgcgccg
    14581 ccgaaggcgt agggaaactg atactcgacg tagcggaagc cggcgcgcgc cgcggcagcg
    14641 aagcgctcca taaagggaac ttccgggaac atcagggaaa cattggcggc gaggcggggc
    14701 atgcgcgact atgttagcta agattgcgcg aatacgagaa gattcctccc accttgccgc
    14761 ttccgcccgc cgtcgcgcgc tttctgccgt ttctcgcctg gtggccgcgc gtcacccgcg
    14821 ccaccctgcg cgccgacgcc agcgccgcgc tgaccagcgc cattatcgtg ctgccgcagg
    14881 gcgtcgcgtt cgccacgctc gccgggctgc cgccggagtt cggcctgtac tgcgcgatgg
    14941 tgcccgcgat ggtggcggcg ttcttcggct cgtcgtggca cgccgtgtcc ggccccacca
    15001 acgcgatctc gctgtacgtg ttcgccacgg tggcgccgct cgccgtgccc ggatcgcccg
    15061 agtacatcag cctggtgctg acgctcgcct tcatgtcggg cgcgctgatg ctggtgatgg
    15121 gtgcgtttgg cctcggccgc ctggtcaact tcgtctcgca cacggtggtg gtcggcttca
    15181 ccgccggcgc ggcggtgctg atcgtcgcga gccagctgcg aaacttcttt ggcatcgacg
    15241 tgccggcggg gagctcgttc ctgtcgaccc tgcgcgcgtt cgcagcgcag gccggcgcgg
    15301 tgcagccctg ggtgatgctg gtgggcgcgc tgacgctcgt cgccggcgcc gcggcgcgcc
    15361 gctacgtgcc gaaggccccg tacatgatcg tcgcgctggt cgcgggcggg ctcgccggcc
    15421 tcgtcatcaa cctttggctg acgcccgagg tgagcggcat ccgcaccatc gggctgctgc
    15481 ccggcgcgct gcccggcctg acgctgcccg atctgtcggc cgacaccctg aagtcgctga
    15541 tgggcatcgc cgtcggcatc accgtgctgt cgctcaccga ggcgatgtcg atctcgcgcg
    15601 ccgtggcgct gaagtcgggc cagcgcatcg acgccgacca ggagttcatg ggccagggcc
    15661 tggccaacat gtcggccagc gtgttctccg gctaccccgt atcggcgtcc ttcaatcgca
    15721 gcggactgaa ttacgaggcc ggagcgcaga cgccgctcgc cgccgcgctg tcggcgccgc
    15781 tgctcgcggt ggtcgtattc gcggtggcgc cgctgcttgc ctacctgccg ctcgcctcga
    15841 tggcggcgct gttgttcatg gtggcctggg gcctgctcga cttcggcgcc atgcgcgcca
    15901 tcgtgcgcac cagccgcagc gagtcggccg tgctcggcct caccttcgcc gcgacgctgc
    15961 tgatgaacct ggaggcggcg atcctggtcg gcgtgaccct gtcgctgatc gtctacctca
    16021 accgcacctc gcggccgcag atgcgcgccg tggcgcccga cccgcgccac agcgtgcgcc
    16081 gcttcggtcc ggtggcgcag ggcatcaccg agtgccccca gctgaagatc atggcagtcg
    16141 agggctcgat ctacttcggc gccgtggacc acgtcgagtc gcatttcgac accctgcgcg
    16201 cggtggcgcg cgagcagaag cacctgctgg tggtggcgcg caacatcaac ttcctcgaca
    16261 tcgccggcgc cgaggcgctg gtgcgcgagg cgcgcgcgcg gcgctggcgc ggcgggcggc
    16321 tgtggctgca gggcctgcgc ctgccggccg aggacgtgct gcgcaacggc ggctaccttg
    16381 ccgagatcgg caccgacgcg gtgttccgcg acaagcgcga ggccatcgcg cgcatcttcg
    16441 acaggctcga ccccggcatc tgcgcgcgct gccgggcgcg catcttcgag gaatgcccga
    16501 cgcggccgct gcgcgaagac agctgagggg gatggttgcc aagcatgcgt cggcgcgact
    16561 agactgccac gtaccttacc cacggagggg gattccatga atgctcgcat tacgattgcc
    16621 gcctgcgccg tcgccgcgct ggcgagcggc acgctgcatg cccagtccac gctcgacgcg
    16681 gtcaagtcca agggctacgt gcagtgcggc gtcaacaccg gcctggccgg gttctcccag
    16741 ccggactcgc gtggcgtatg gaagggcatc gacgtcgata cctgccgcgg ggtcgcggcc
    16801 gctgttttcg gcgacgccac caaggtgcgc tacacgccgc tcaccgcgca gcagcgcttc
    16861 acggcgctgc agtcgggcga gatcgacgtg ctggcgcgca acaccacctg gacggtcagc
    16921 cgcgacacca gcctcggcct gaatttcgtc ggcgtgaatt actacgacgg ccagggcttc
    16981 atggtgccga agaagctcgg cgtgaagagc gcgaagcaat tgaacggcgc caccatctgc
    17041 gtgcagcccg gcaccaccac cgagctcaat ctcgccgact atttccgcgc caacaagatg
    17101 agcttcaagc ccgtggtgat cgagaagctc gaggaagtca ccaacgccta cttctcgggc
    17161 cgctgcgacg tgttcaccac cgacgtctcg ggcctcgttt cgacgcgcgc ctcgcgcgcg
    17221 cccaacccgg ccgatcacgt gatcctgccc gaggtcatct ccaaggagcc gctcgggccg
    17281 gtggtgcgtc acggcgatga ccgctggttc gacgtcgtca agtggacgct gttcgccatg
    17341 ctcgaggccg aggagctggg cctgtcctcg aagaacatcg actcgcaggt gtcgagcacc
    17401 aacccgtcgg tgcagcgctt cgtcggcgcc accggagaca tcggcaagat gctggggctg
    17461 gacgcacgct gggcctacaa cgtcgtcaag caggtgggca actacggcga atcgtttgac
    17521 gccaacctca cccccctcgg cttcgaccgc ggcatcaaca acctgtggac ccagggcggc
    17581 ctgatgtacg cgccgcctct gcgctagaac gcaccgacag ttgcgccccg tggcgggcga
    17641 cagcagacag ggggcgcgcg ccgctgccaa gcggcgcgcg tcttggtcgc tcaacaaccc
    17701 gggagtccgc gccctcatct accaggtggc gctggtcgcg gcggtgctgc tgggggtctg
    17761 gtacctcgcc agcaacaccg cgcacaacct cgaggtgcgc aagatcgcga gcggtttcgg
    17821 cttcctcggc cgcgaagcgg gattcgagat tggcgaatcc ccgttcaccg cctacggcgc
    17881 cgccgacacc taccgcaagg ccttcgtcgt cggcctgctc aacactttcc gcgtcgcgct
    17941 gatcggcatc gtcctcgcca ccctgatcgg cacgctgatc ggcctggcgc ggttgtcgca
    18001 caactggctg atcgcgaaga tctcctcggg ctacatcgag acggtgcgca acatcccgct
    18061 gctggtgcag ctgttcttct ggtacgcgat catctcggaa aacctgcccg gcccgcgcga
    18121 agcgctcaac ccgctgccgg gcgtgtacct gagcaatcgc ggcatcgtgt tcccggtgtt
    18181 cgcttcgcac ccggtgcatg tgtggatggg cgtcactctg ctcgccggga ttgcggctgc
    18241 gtggctggtc gcgcgctggg cgcgcgcgcg ccaggcgcgc accgggcaga tcttcccggt
    18301 cttctcaacg ggtgcggcgc tgatcctcgg cctgccgctt gtcgcgttcc tggccgccgg
    18361 cgcgccgacc gcgctcaacc gcccggagct cgccggcttc aatttcgcgg gcggcggcgt
    18421 gctcacgccg gagttcgccg cgctgctcgc gggcctcacg ctctacaccg cggcgttcat
    18481 cgccgagatc gtgcgcgccg gcatcatcgc cgtcgatcgc gggcagttcg aggccgccgc
    18541 ggcgctcggc ctgcggccgc gccgcgtcat gcgcctggtc atcctgccgc aggcgctgcg
    18601 cgtcatcgtg ccgccgatga ccagccagta cctcaacctc accaagaaca gctcactcgc
    18661 ggtggccatc ggctaccccg acctggtgtc gatcgccaac acggcgatga accagaccgg
    18721 gcaggcgatc gagggcatcg cggtgatcat ggccgtttac ctgacgatca gcctgtcgat
    18781 ctccgcgttc atgaactggt acaacgcgcg catcgcgctg aagggagcgc gccggtgagc
    18841 gccagacatg cgcccgcggg cgcctcgccg ctgtcgtgga tcatcggcaa cctgttctat
    18901 agcgtgccga gcgcgctggc cacgctgctg ctcgcctacc tcgcctggaa ggcgatcgtg
    18961 cctttcgtcg actgggcgtt cgtcaatgcc atctggagcg cgcccgactc caagccgtgc
    19021 cgcgaggcgc gcggcgccgg cgcctgctgg gcgctgatca ccgaaaaaca ccgctttatc
    19081 ctgttcggca cctatccgta cgagcagcac tggcgtccgg cgctcggcat cctgctgctg
    19141 atcgcgctgt acgtggtgag cgcgatgcgc cgcttctggc gcccggcgct cgccggcgtg
    19201 tggctcgccg gtctggcgct gatcggcgta ctgatgtggg gcggcgtatt cggcctgccg
    19261 tacgtggaga acgagcgctg gggcggcctg atgctcacgc tgctcctggc caccatcggc
    19321 atcgcgtttg cctttccgct gtcgatcgtg ctcgcgctcg ggcggcgctc ggacatgccc
    19381 gcggtgcgcg tgctgtgcgt cggctacatc gagctgatcc gcggcgtgcc gctgatctcg
    19441 ctcctcatca tggcctcggt gatgctgccg ctgttcctgc ccgagggcct gagcatcgac
    19501 aagctgctgc gcgcgcagat cgcgatgatc ctgttcgcgg cggcctacct ggccgaggtg
    19561 gtgcgcggcg ggctgcaggc ggtgccccag ggccaggtcg aggccgccga cgccatgggc
    19621 ctgtcgtact ggaagaagac gctgctcatc gtgctgccgc aggcgctgcg cgtgtcgatt
    19681 ccgccgctgg tgaacacctt catcggcttc ttcaaggaca cttcgctggt cgtgatcatc
    19741 ggcctgttcg acctgctcac ctcgatcaag gtgtcgatcg ccgagcccgc ctggaccggg
    19801 ttcggcgtcg aggcctacat cttcgcttcg ctcgtgtatt tcgccttctg ctacgccatg
    19861 tcgcagtaca gccagaatct ggagcgggag ctggcgcgcg gccgggcaca ctacctcagg
    19921 gagaactgac cgtggcccag ccgatgatcg agatcaaggg cgtcaacaag tggttcggcg
    19981 cgttccacgt gctgaagaac gtcgagctgt cggtggccca gggcgagaag atcgtcgtct
    20041 gcggcccgtc aggctcgggc aagtcgacgc tggtgcgcct gatcaaccgc ctggagtcgc
    20101 accaggaggg ccgcatcgtg gtcgacggca tcgagctggc cgaagatctg aaggcgatcg
    20161 aggccatccg ccgcgaagtc gggatggtgt tccagagctt caacctgttc ccgcacctga
    20221 cggtgctcga gaacctgacc ctggcgccca tctgggtacg gcgcatgccg aagaaggacg
    20281 ccgaagagct tgccatgcag ctgctcaagc gcgtgcgcat cgccgagcag gccgcaaagt
    20341 acccggcgca gctttccggc ggtcagcagc agcgcgtcgc gatcgcgcgg gcgctcgcca
    20401 tgaggcccaa ggtgatgttg ttcgacgagc cgacctcggc gctcgacccg gagatgatca
    20461 aggaagtgct ggacgtcatg atcgagctcg cgcaggaggg catgacgatg ctggtggtga
    20521 cgcacgagat gggcttcgcg cgcaccgtgg ccgaccgcgt ggtgttcatg gaccagggcg
    20581 agatcgtcga gtcgggggcg ccgaaaactt tcttcgaggc gcccaagacc gaccgcgcca
    20641 ggctgttcct cagccagatc ctcgggcact agctatgcta gacttttttt gcccttaacc
    20701 acaagcacgc aaaggagatt tccatgggcg cgatactttc gtccctcagc cgcacgatcg
    20761 ttgccggcat cgtcgtgctg gtggtcatcc tgctcatcgt gggccaggcg accggcgcct
    20821 ggatcaacct gggcagccac gcctggtggg cgttcttcat gcgctggctg cacgtgctct
    20881 ccggcgtgat gtggatcggc ctgttgtggt acttcaactt cgtgcagacg ccgtcgatgc
    20941 ccaagatccc cgacgagcac aaacccgcca tcagcaaggt catcgctccg gaggcactgt
    21001 tctggttccg ctgggcggcg ctggcgacga tcgtgaccgg cctgctcctc gccaccatga
    21061 gcggctggct ggtgcaggcg ctctcgctgc agcgcccgtt caccgccatc ggcatcggca
    21121 tgtggctcgg cctggtgatg gcgttcaacg tctggttcat catctggcca aaccagaaga
    21181 aggcgctcgg catggtgcag gttggcgccg aggagaaggc cgcggcggca aagaaggccg
    21241 gcatgacctc gcgcctgaac acgctgctgt cgatccccat gctctactgc atggtggcgc
    21301 agcagaatgg gggcctgtag ctagcgcggc ttgagctggt ccagcccgcg caggcgaaac
    21361 cctgcgcgga tgccgcgcgc ggcgaaccag ccctgcgtca tttccagcgc aaagcgcgcg
    21421 ggccgcgccg cgcagtgcga gtcctcggtc tgcggctgca tgtcggcgat gttgacgatg
    21481 gcgccgcgct cgtcgatgaa ggccaccgac agcggcagca gcgtgttctt catccacatg
    21541 cagtgcaccg aggtttcgtc gaatacgaac agcatgccgc cattgggcgc gagcgaggtg
    21601 cgcagcatga gacccatggc gcgggcggcg tcgtgatcgg cgacctcggc gcggatcagg
    21661 tgcatgccag cgccgagctg gaccgtcggc aacgtgggct gctgcgcatg gccgccggct
    21721 gcggcgagca gcaggacaag gaaaaggcgc ttcacgggca acaaaaaagg gcagggtagc
    21781 aaccctgccc ttttcgggaa agaggcgact tacttcttgt cgccttcctt tgcttccttc
    21841 gcttccttct tcttggccgc cttcttcttg tcggccttct tctccacctt cttctcgcct
    21901 tccttcatcg gcgcgccggc gtgcgacgcg gcaaaggcgg aggcgctaac ggccgcgaac
    21961 atcgcggcgg cgatcacagt cagaagcttg ttcattcaaa tctcctagtt ggtgtacacc
    22021 gtagcgggtc ggcgcagcgc ttccgccacg ctcccgcagt caataacgcc gcacgtgcgc
    22081 agcggttgac cgagtcgggg gcctttgtcc tattttttgt aacctcatga tattattggg
    22141 attaacccct ttcccgtgac agtcttcagg ttcctgattg ggacactgac gtgccttgcc
    22201 accgtgcagg ccgcggctgc gccgcgcttg ccgtcggatg atgcggaaat cctcgagcgc
    22261 ctgtcgctgg tgcgcgacag tccggaatcg gccgcgctgc gccagttgcg cgcccgtgtc
    22321 gccgccgccc ctacggatcc cgctcccgcc gcggcgctcg cgcgtcgcta tttcggcctc
    22381 gcgatggcgc agggcgatcc ccggttcgtc ggctacgcgc aagcggtcct gcggccctgg
    22441 gacgaggcac tcgcgcctgc cgacatactg ctcgtgcgcg cgatgttgcg gcagtaccgg
    22501 cacgatttcg acggtgcgct gcgcgcactg gcgctcgccc tggagcgcga tcgcaacaat
    22561 gccgagggcc gtgcctggcg ggcggcgatc ttcatggtgc gcgcggagta tgggaaggcc
    22621 gccggcgaat gcgccgcgct cgcggcgctg agcgacgacc tgctggccgc gggctgcagc
    22681 gcgtatgtcg aggccacgac gggccgtacg cgcgctgcgt acgaccggct gcgcgcggcg
    22741 ctcgaacaac acgccgacgc cgacatgggc gtacgcctgt gggtgcagac gcggctcgcg
    22801 gaaatggcgg atcgcctcgg cgacgccgcc gcggccgaga atcactttcg cgccgcgctc
    22861 gcgctcggca tcgacgacaa cttcctgctc gccgcgtacg cggacttctt gctggagcag
    22921 ggccgccagc gcgaggtcgt ggcgctgctc gaggaccgcg gccgttcgga caccctgttg
    22981 ctgcgccttg cgctcgccat gcacgagctc ggtatgcccg aagcggcaaa gcaggcgcgt
    23041 gtgctcgccg agcggttcgc cgccgcggcg ctgcgcggcg accggctgca cctcgcggaa
    23101 gaagcccgct acctgctcga gcttcggcgc gatgccggtg cagcacttgc cgccgccgcg
    23161 gagaactgga agtcgcagcg cgagccgcgc gatgccctga tactgctgcg ggcggcgcgc
    23221 gcggcgcgcg ccccggccgc cgcggccccg gtactcgact ggctagcgcg aaccggattc
    23281 gagcacgacg gcatgcgccg cctagccgca gagttgcggt gaaggccgtc gtcttgttcc
    23341 tcgcgctggc attcgccggc ggcccggcgc ttgcgcacaa gccgagcgac agttatctcc
    23401 gtgtgcgggt cgagggcgcg ctgctgcgag ggcagtggga catcgcgctg cgcgaccttg
    23461 agtacgccat cggcctggat gccgatggcg atggcgccat aacctggggc gaggtgcgcg
    23521 cgcgcgaggc cgacatagcg gcctacgcgt tcgcgcggct tgcgttgagc gccgacggcc
    23581 agacttgcgg cgccgagtcg cgcaggttgc tcgtcgacga gcacagcgac ggcgcctacg
    23641 ccgtactgga attcgaggcg cgttgcgccg ccgcgccgca gcgcaccctg cggttcgact
    23701 acagcctgtt cgccgacctc gaccccacgc accgcggcct gctccaggtg cagcaaggcg
    23761 ccgcgaccgc gagcggcgtg ctgggcccgg accacccgcg cctcgagctc gcggttggca
    23821 cggtgtcgcg cctgcgccag ttcctggatt acgcgcgcga gggggtgtgg cacatctgga
    23881 tcggcatcga tcacgcgctg ttcctggtat cacttctgct ccccgcagtg ctggtttacg
    23941 gcgcgcgcgc ctgggagccc gcgccacgct ttgcgcccgc gttctgggac gtcttcagga
    24001 tcgttaccgc gttcaccgtg gcgcattcca tcaccttgtc gcttgcggcg ctcggcgtcg
    24061 tggcgctgcc ctcgcgcctc gtcgaatcgg tgatcgcgct ctcggtcatg ctcgccgcgc
    24121 tcaacaacct gaggccgtgg gtgctcggcc ggcgctgggc ggtggctttc ggcttcgggc
    24181 tggtgcacgg ctttggcttt gccagcgtgc tcgccgacct cgggctgccg cgcgaggccc
    24241 tgctcgtgtc actggtcggc ttcaacgtgg gtgtcgaagc tgggcagctc gcaatcgtcg
    24301 gcgcatttct gccggcggcg ttccttgcgc gtcgtcgttg ggcctatcgc cgcctggcgt
    24361 tcgcgggcgg ctccgttgcg atcgcactgc tggccgccgc gtggctagtg gagcgggcgt
    24421 tcgacctgaa gctcttataa tggcgcccat tcgacgcatc cacgcaggag ctttcatggc
    24481 atacaagcac atcaaggtcc ccgccaccgg cgagaagatc aaggttaaca aggacttttc
    24541 tttggcggtg cccgacactc cggtgattcc ctacatcgag ggcgacggta ccggcgtcga
    24601 catcacgccg gtgatgatca aggtggtgga cgcggccgtg aagaaggcct acggggcgaa
    24661 gaagaagatc gtctggatgg agatgtacgc cggcgagaag tcctgcaagg tctacggcga
    24721 gaacgtatgg ctgcccgacg agacgctcgc cgcgtccagg gaatacgtcg tgtcgatcaa
    24781 aggccccctc accacgccgg tgggcggcgg catccgctcg atcaacgtgg cgctgcgcca
    24841 ggagctcgac ctctacgtgt gcctgcgccc gatccgctac ttcaagggcg tgccctcgcc
    24901 gctcaaggag ccggagaaga ccgacatggt gatcttccgc gagaactccg aggacatcta
    24961 cgccggcatc gagtgggcgg cgaagacgcc cgaggtgcag aagctgatca agtacctgac
    25021 cgacgagatg aaggtgaaga agatccgctt tcccgagacc tcggccatcg gcatcaagcc
    25081 ggtctcggtg cagggctcgg agcggctgat ccgcaaggcc ttccagtacg ctgtcgacaa
    25141 cgaccgcaag tcggtgaccc tggtacacaa gggcaacatc cagaagttca ccgagggggc
    25201 gttccgagac tggggctacg ccctggcgca gaaggagttc ggcgcgaagc tggtcgacgg
    25261 cgggccgtgg tgcagcttca agaaccccaa gaccgggcgc gagatcgtgg tgaaggacgt
    25321 gatcaccgac gcgttcctgc agcagatcct gctgcgcccg gcggagtacg acgtgatcgc
    25381 caccatgaac ctgaacggcg actacgtctc ggacgcgctt gccgcgcagg tcggcggcat
    25441 cggcatcgcg ccgggcgcca acatgagcga ctcgatcgcc atgttcgagg ccacccacgg
    25501 caccgcaccg aagtacgccg gcaaggacta cgtcaatccg ggctcggaga tcctgtccgc
    25561 cgagatgatg ctgcggcaca tgggctggac cgaggccgcc gacgtaatcg tgcgctcgat
    25621 ggagaaggcg attgccgaca aggtggtgac ctacgacttc gcgcgcctga tgcagggcgc
    25681 caagcaggtg tcgtgctccg gcttcggcca ggcgatgatc gagcgcatgt agcagcgcgc
    25741 cggcgccgcc gcaaagaaaa agggccgctg gcgcggccct tttcgcttgc ggatgcggcg
    25801 ctcagacctt gttgatgttc gaggcctgct tgcccttggg cccctgcacc acgtcgaagc
    25861 tcaccttcat gccctccttg agggtcttga agcccgccat ctggatggcc gagaaatgcg
    25921 cgaacaggtc ttcgctgccg tcatccggcg tgatgaagcc gtaccccttc gcgtcgttga
    25981 accacttcac ggtgcctgtc gccattgccg catcccttgt gttggttgga gtacgccgga
    26041 cacctcgccc gggccatgct tgaaactcac gtcgccgccg ccctttccta tggcgttaca
    26101 gacagcctga atccaaacac aaggcggctt ctacccggct tcccagaacc cgtcaagacc
    26161 cccctggcgg cgcgttgcgc gcgccgaaca cattgattag gatagccatc agcgggcgcg
    26221 gttatgcccg gcgattcaca cattgcacgg agtgcgggaa cggaaactta aagggcatgt
    26281 ccgatcggat tgagcttggc agcgactctt aagcggcatg gcgcagttca aggacgacat
    26341 tctgctcgag gcgcagcgga gcaagctgca gccgccgccg atgttcaagg tcatgctgct
    26401 caacgacgat tacacgccga tggagttcgt ggtgatggtg ctgcagaagt tcttcggcat
    26461 gacgcgcgag cgtgccacgc aggtcatgct gaaggtgcat cgcgagggca tcggggtgtg
    26521 cggcgtgtat cctcaggata tcgccagcac caaggtgcag caggtcgcgg ggtacgcgcg
    26581 gcgccaccag catccgctgc aatgtgtgat ggaggaaacg tagcatgatc gcccaggagc
    26641 tcgaagtcag cctgcacatg gcgttcgtcg aagcgcgcca gaagcggcac gagttcatca
    26701 ccgtagagca cctgctgctg gcgctgctcg acaaccccac ggcggccgaa gtgctgcgcg
    26761 cctgcggcgc caatctggac gagctgcgca agagcctgac ccagcacgtc gccgagcaga
    26821 cgccgcgcat cgccgccgat cgcgaggttg acacgcagcc caccctcggc ttccagcgcg
    26881 tcatccagcg cgccatcctg cacgtgcagt cctcgggcaa gaaggaagtc accggcgcca
    26941 acgtgctggt ggcgatcttc ggcgagaagg attcgcacgc ggtgtacttc ctgcagcagc
    27001 agggtgtgac ccggctcgat gtcgtcaatt acatctcgca cggcatcacc aagacgccgc
    27061 agccgcacgc cggcaaggcc gagcaggagg ccggcgacga ggcgcaggcc gagccctcca
    27121 acagcccgct cgacaactac acgcagaacc tgaacgcgc
//
LOCUS       DBNHKE010000133         3905 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_763388, whole genome shotgun sequence.
ACCESSION   DBNHKE010000133 DBNHKE010000000
VERSION     DBNHKE010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_763388"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3690)
                     /gene="treY"
                     /locus_tag="AC16O9_08925"
     CDS             complement(<1..3690)
                     /gene="treY"
                     /locus_tag="AC16O9_08925"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HIR6516778.1"
                     /translation="MAEARESLEHLAARYGIAAEYHDIWGKRHTASDATLAALLAQFE
                     PHAGPEPALPPAAAIAADAARWSLPLGVAGEARTLRWRVAEEGGAIHEGEAGCAPPDF
                     RLELPLPLPPGYHRLSVEGLSGETLLICAPARCYRPPSLADGGRVWGPAVQLYALRSE
                     RNWGIGDFGDLAKLVELAAERGAGIIGLNPLHALFPHNPAHASPYSPSSRLQLNVLYL
                     EVEAIEEFRECEAAQRRVRSAQFQARLAQLREAPLVDYAGVAAAKFEILELLYAHFRE
                     RGARAEFRGFQDKSGRALRLHATYEALQAHFHAQSAATWGWPVWPEAFRSHDSAEVAR
                     FAAENLERIEYYEYLQWQAERQLAQASERCRARGLAVGLYLDLAVSVDRAGSDTWTQQ
                     ECYALGASVGAPPDEFNPNGQNWGLPPLRPDRLRAQGYRLFIETLRENMRDAGAVRID
                     HVMGLMRLFWIPPGKGAAEGTYVSYALEEMMAIVALESERARCMVIGEDLGTVAEEMR
                     AALARYEVLSYRLLYFERGEGGIFKSSADYPRDALVAVSTHDLATLAGWWIGHDLRVR
                     LELGLFPNEGVYEKQLIDRAQERVRMMLALQHAGLLPEGVVVEPTGSQALTPALAEAI
                     HAFVAAAPSRVMMVQLEDVIGATEQANLPGTTEKHPNWKRKIPQTLEQLAADERLRSL
                     AATLAKIRPHPAPRAAAGPAAEARVPRATYRLQFNKDFTFDDAVKILPYLARLGVSHV
                     YCSPILRARPGSMHGYDIVAHDEINPELGGAEGYERFSAALRQHGMGQLLDMVPNHMG
                     VLGADNAWWMDVLENGQASAYAHYFDIDWYPVNADLRGKVLVPALGDHYGNVLAGKDL
                     VLAFERDAGAFALRYHEHRFPLDPRTYPVLLQRAEALLPQGEARAALSSLSAAFGHLP
                     ARDAADAAAIAERVRDKEVHKARLARLAGREAAVASALDAAVAEVNAPGPRDALHALI
                     EMQPYRLAYWRVATDEINYRRFFDINDLAALRMESEQVFEATHAFVLELAAKGIVDGL
                     RIDHPDGLYDPAQYFRRLQEGYARRAGIPLPERDTRGRPARPLYVAVEKIVAPHEDVP
                     ETWHVHGTTGYRFAMVANGVLVDTAAQARFDRIWHAFSREDENFEELAYAGKRAIMRS
                     ALASELTVLSTELLRIARADRRTRDYTFNTLRQALAEVAACMPVYRTYIIDAASDQDL
                     RYVNWAVAHARRRSRAADVSIFDFVR"
     gene            complement(3683..>3905)
                     /locus_tag="AC16O9_08930"
     CDS             complement(3683..>3905)
                     /locus_tag="AC16O9_08930"
                     /inference="COORDINATES: protein motif:HMM:NF023367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HIR6516779.1"
                     /translation="RFACEGALLRVEWTLGDGSRWRLLANFGSGDAETGEQHPAGSPV
                     YAAGVQAAPGALLRLAPGAVRFTLEAARG"
BASE COUNT          520 a         1388 c         1449 g          548 t
ORIGIN      
        1 gcgcacgaaa tcgaagatcg acacgtcggc cgcccggctg cggcgccggg catgcgccac
       61 cgcccagttg acgtagcgca ggtcctggtc cgaggccgcg tcgatgatgt aggtgcggta
      121 caccggcatg caggcggcga cctcggcgag cgcctggcgc agcgtattga aggtgtagtc
      181 gcgcgtgcgc cggtcggcgc gcgcgatgcg caggagctcg gtcgagagca ccgtgagctc
      241 ggaggcgagc gcgctgcgca tgatggcgcg cttgccggca taggcgagct cctcgaagtt
      301 ctcgtcctcg cgcgagaagg cgtgccagat ccggtcgaac ctggcctgcg ccgcggtgtc
      361 caccagcacg ccgttggcca ccatggcgaa gcggtagccg gtggtgccgt gcacgtgcca
      421 ggtctcgggc acgtcttcgt gcggcgcgac gatcttctcc accgcgacgt aaagcggccg
      481 cgccggccgg ccgcgcgtgt cgcgttccgg cagcgggatg ccggcgcgcc gcgcgtagcc
      541 ttcctgcagg cggcgaaagt actgcgccgg gtcgtacagg ccgtcgggat ggtcgatgcg
      601 caggccgtcc acgatcccct tggccgccag ctcgagcacg aaggcgtggg tggcctcgaa
      661 cacctgctcg ctttccatgc gcagcgccgc gaggtcgttg atgtcgaaga agcgccggta
      721 gttgatctcg tcggtggcga cgcgccagta ggcgaggcgg tagggctgca tctcgatcag
      781 cgcgtgcagc gcatcgcgcg ggccgggcgc gttgacttcc gcgaccgcgg cgtccagagc
      841 gctcgccacc gccgcctcgc ggccggcgag gcgcgcaagg cgcgccttgt ggacctcctt
      901 gtcgcgcacg cgctcggcga tggccgcggc atcggcggcg tcgcgcgccg gcaggtggcc
      961 gaaggccgcg gacaggctgg agagcgccgc gcgcgcctcg ccctggggca gcagcgcctc
     1021 ggcgcgctgc agcagcacgg ggtaggtgcg cgggtcgagc ggaaagcggt gctcgtggta
     1081 gcgcagcgca aaggcgccgg cgtcgcgctc gaaggcgagc accagatcct tgcccgccag
     1141 cacgttgccg taatggtcgc cgagcgccgg caccagcacc ttgccccgca ggtcggcgtt
     1201 caccgggtac cagtcgatgt cgaagtagtg cgcgtaggcc gacgcctggc cattctcgag
     1261 cacgtccatc caccaggcgt tgtcggcgcc gagcaccccc atgtgattgg gcaccatgtc
     1321 gaggagctgc cccatgccgt gctggcgcag cgccgcgctg aagcgctcgt agccctcggc
     1381 gccgccaagc tcgggattga tctcgtcgtg cgcgacgatg tcgtagccgt gcatgctgcc
     1441 cggacgcgcg cgcaggatcg gcgagcagta cacgtggctc acgcccaggc gcgcgaggta
     1501 cggcaggatc ttcacggcgt cgtcgaaggt gaagtccttg ttgaactgca gccggtaggt
     1561 ggcgcgcggc acccgcgcct cggcggccgg gccggcggcg gcgcgcggcg ccgggtgcgg
     1621 gcggatcttc gccagcgtcg cggcgaggct gcgcaggcgc tcgtcggcgg cgagctgctc
     1681 gagcgtctgc gggatcttgc gcttccagtt cgggtgcttt tccgtggtgc ccggcaggtt
     1741 ggcctgctcg gtggccccga tcacgtcctc gagctgcacc atcatcacgc gcgaaggcgc
     1801 cgctgcgacg aaggcgtgga tcgcctcggc gagcgccggc gtcagcgcct gcgagccggt
     1861 gggctcgacc accacgccct ccggcagcag cccggcgtgc tgcagcgcga gcatcatgcg
     1921 cacgcgctcc tgggcgcggt cgatgagctg cttttcgtac acgccttcgt tcggaaacag
     1981 gcccagttcc aggcgcacgc gcaggtcgtg cccgatccac cagccggcga gcgtcgcgag
     2041 gtcgtgcgtg ctcactgcca ccagcgcatc gcgcggataa tcggcgctcg acttgaagat
     2101 cccgccctcg ccgcgctcga agtacagcag ccggtacgag agcacctcgt agcgcgcgag
     2161 cgccgcgcgc atctcctcgg ccacggtgcc gaggtcctcg ccgatcacca tgcagcgggc
     2221 tcgctcgctc tccagcgcga cgatcgccat catctcctcg agcgcgtagc tgacgtaggt
     2281 gccctcggcg gcgcccttgc cgggcgggat ccagaacagg cgcatcagcc ccatgacgtg
     2341 gtcgatgcgc accgcgcccg cgtcgcgcat gttctcgcgc agggtctcga tgaacaggcg
     2401 gtagccctgc gcccggaggc ggtcgggcct gagcggcggc aggccccagt tctggccgtt
     2461 ggggttgaat tcgtccggcg gcgcgccgac gctcgcgccc agggcgtagc actcctgctg
     2521 cgtccaggta tcggagccgg cgcgatcgac cgatacggcg aggtcgaggt acaggccgac
     2581 ggcgaggccg cgcgcgcggc agcgctcgct cgcctgcgcg agttgccgct cggcctgcca
     2641 ttgcaggtac tcgtagtact cgatgcgctc gaggttctcg gcggcgaagc gcgcgacctc
     2701 ggcggaatcg tggctgcgaa aggcctcggg ccagaccggc cagccccagg tggcggccga
     2761 ttgcgcgtgg aaatgcgcct gcagcgcctc gtaggtcgcg tgcagccgca gcgcgcgtcc
     2821 gctcttgtcc tggaatccgc ggaactcggc ccgcgcgccg cgctcgcgaa agtgcgcgta
     2881 gagcagctcg aggatctcga acttggccgc cgccacgccc gcgtagtcca cgagcggcgc
     2941 ctcgcgcagc tgcgccaggc gcgcctggaa ctgcgccgag cgcacgcggc gctgcgccgc
     3001 ctcgcactcg cggaactcct cgatcgcctc gacctcgagg tacagcacgt tgagctgcag
     3061 gcgcgacgac gggctgtagg ggctggcgtg cgccggattg tgcggaaaca gtgcgtgcag
     3121 cggattcagc ccgatgatgc cggcgccgcg ctcggcggcg agctcgacca gcttcgccag
     3181 gtcgccgaag tcgccgatgc cccagttgcg ctccgagcgc agcgcgtaga gctgtaccgc
     3241 ggggccccag acgcgcccgc cgtcggcaag tgacggcggc cggtagcagc gcgccggcgc
     3301 gcagatgagc agcgtctcgc cgctcaggcc ttcgacgctg aggcggtggt atccgggcgg
     3361 cagcggcagc gggagctcga gccggaagtc gggcggcgcg cacccggcct cgccctcgtg
     3421 gatcgcgccg ccttcctcgg cgacgcgcca gcgcagggtg cgcgcttcgc ccgcgacgcc
     3481 gagcggcagc gaccagcggg ccgcgtcggc ggcgatcgcc gccgccgggg gcagcgccgg
     3541 ctcggggccc gcgtgcggct cgaactgcgc gagcagcgcg gcgagcgtgg cgtcggaggc
     3601 cgtgtgccgc ttgccccaga tgtcgtggta ctcggccgcg atgccgtagc gcgcggcgag
     3661 gtgctcgagg ctctcgcgcg cctcagccac gcgccgcttc gagcgtgaag cgcacggcgc
     3721 ccggcgcgag gcgcaagagc gcgcccggcg ccgcctgcac ccccgccgcg tacaccggcg
     3781 agccggccgg gtgctgttcc ccggtttcgg cgtcgccgga accgaagttc gcgagcaaac
     3841 gccagcggct gccgtcgccg agcgtccact cgacccgcag cagcgcgcct tcgcaggcga
     3901 agcgg
//
LOCUS       DBNHKE010000134         2282 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_974663, whole genome shotgun sequence.
ACCESSION   DBNHKE010000134 DBNHKE010000000
VERSION     DBNHKE010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_974663"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1151
                     /locus_tag="AC16O9_08935"
     CDS             120..1151
                     /locus_tag="AC16O9_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6516780.1"
                     /translation="MKPVKLAAMLAAASALAAAGTAAQAQTVLTMSNWVPPAHPIVVY
                     IMKPWADQVAKVTKGRVRVQMLPKAIAAPNQHYDATINGQVDLAWATYGYQPERFQPY
                     LIAELPQLGETAVHNGVALWRTHKKFIEQSGIHKDVQLLGVMTHGPGLFHHSKKHILV
                     PADLQGQKMRVGGDVPKAIVESFGGVVITQPAPKSYEILSAGIADGIMFPTESLKSFK
                     IAGLVKHTSYVKGGLYSSSFWFAMNKKKFEGLSQADQDAIMKVSGEKFARLAGEGWDR
                     ADKEGRVAMKEAGGTIREVKPEFVAELKKVTERLERDYGAKMDAMGIDGAGAIKFFRA
                     EVARVAAGK"
     gene            1272..1691
                     /locus_tag="AC16O9_08940"
     CDS             1272..1691
                     /locus_tag="AC16O9_08940"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6516781.1"
                     /translation="MTLTFLDVTGRKFLASPIYGAYEITEFMMGVLIFSALPLVTARE
                     NHVTIDVFDHFIAPAIGRWQRVIVNLLSTVVLAFLGWRLWILSASHLRTNEVTMTLHI
                     PHGPFSRAFAVLAILAAIACLVVALEHIERARLRTTQ"
     gene            1688..>2282
                     /locus_tag="AC16O9_08945"
     CDS             1688..>2282
                     /locus_tag="AC16O9_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR6516782.1"
                     /translation="MTETLVAFAVLLALAFARMPIAFAMLIVGGAGFALKRGWPAAFD
                     QVAQTTFAAGIDYELSVVPLFILMGNFVARAGMSQELFRAAYTFIGHWKGGLAMSTVV
                     ACAGFGSICGSSIATAATMSRVAYPPMKKLGYSDKLASASIAAGGTLGILIPPSTIMV
                     IYGIITETNIGRLFAAGVLPGVLAVALLCLAVRWSVWR"
BASE COUNT          374 a          787 c          748 g          373 t
ORIGIN      
        1 tcatagagaa ccgagcccgg gcaccgacgg gttcgcgccg ccggctcaaa taaaatgagg
       61 tctgtcccta tttatcccgc tgctaccata acggcctggt cttaggagga ggagaaacga
      121 tgaaacccgt caaactcgcg gcgatgctgg ccgccgccag cgcgctcgct gccgccggca
      181 ccgccgcgca ggcgcagacc gtgctcacca tgtcgaactg ggtgccgccg gcgcacccga
      241 tcgtcgtcta catcatgaag ccctgggccg accaggtggc caaggttacg aaagggcgcg
      301 tcagggtgca gatgctgccc aaggcgatcg ccgcgcccaa ccagcactac gacgccacca
      361 tcaacggcca ggtggacctc gcctgggcca cctacggcta ccagcccgag cgcttccagc
      421 cgtacctgat cgccgagctg ccgcagctgg gcgagacggc ggtgcacaac ggcgtggcgc
      481 tgtggcgcac gcacaagaag ttcatcgagc aatcgggcat ccacaaggac gtgcagcttc
      541 ttggcgtgat gacgcacggc ccgggcctgt tccaccattc gaaaaagcac atcctggtgc
      601 ccgccgacct gcaggggcag aagatgcgcg tcggcggcga cgtgccgaag gccatcgtcg
      661 aatccttcgg cggcgtggta atcacgcagc cggcgcccaa gtcctacgag atcctgtcgg
      721 cgggcatcgc cgacggcatc atgttcccca ccgagtcgct gaagagcttc aagatcgcgg
      781 gcctcgtcaa gcacaccagc tacgtgaagg gcgggctgta cagctcgtcg ttctggttcg
      841 ccatgaacaa gaagaagttc gagggcctgt cgcaggccga ccaggacgcc atcatgaaag
      901 tctccggcga gaagttcgcg cgcctcgccg gcgagggctg ggaccgcgcc gacaaggagg
      961 gccgcgtggc gatgaaggag gcgggcggca ccatcaggga agtcaagccc gaattcgtcg
     1021 ccgagctgaa gaaggtcacc gagcgcctcg agcgcgacta cggcgcgaag atggacgcca
     1081 tgggcatcga cggcgcgggc gcgatcaagt tcttccgcgc cgaggtggca cgggtcgccg
     1141 cagggaagta gcgccgactg tcggctgcac tgcacgaagc gacccggagg gacgcgctct
     1201 ttccggggcg agccggcgcc tgggccgcct gggcgctcgg caccatcgcc gcgatcgacc
     1261 tcgcggcgat gatgacgctc accttcctcg acgtgacggg gcgcaagttc ctggccagcc
     1321 ccatctacgg cgcctacgag atcaccgagt tcatgatggg cgtgctgatc ttcagcgcgc
     1381 tgccgctggt caccgcacgc gagaaccacg tcaccatcga cgtcttcgac catttcatcg
     1441 cgcccgccat cggccgctgg cagcgcgtga tcgtgaacct cctgtccacc gtggtgctcg
     1501 ccttcctcgg ctggcggctg tggatcctgt cggcgagcca cctgcgcacc aatgaagtga
     1561 cgatgacgct gcacatcccg cacgggccgt tcagccgcgc gttcgccgtg ctcgcgatcc
     1621 tcgccgcgat cgcctgcctg gtggtggcgc tggagcacat cgagcgcgcg cggctgcgca
     1681 cgacgcaatg accgagacgc tggtcgcctt cgcggtgctg ctcgcgctcg cgtttgcgcg
     1741 catgccgatc gcgttcgcca tgctgatcgt cggcggcgcc ggctttgcgc tgaagcgcgg
     1801 ctggcccgcg gctttcgacc aggtggcgca gaccaccttc gccgccggca tcgactacga
     1861 gctctcggtg gtgccgctct ttatcctgat ggggaacttc gtcgcgcgcg ccggcatgtc
     1921 gcaggagctg ttccgtgccg cctatacgtt catcgggcac tggaaggggg ggctggcgat
     1981 gtccaccgtc gtcgcctgcg ccggcttcgg ctcgatctgc ggctcttcga tcgccaccgc
     2041 ggccaccatg tcgcgcgtgg cctacccgcc gatgaagaag ctcggctact ccgacaagct
     2101 cgccagcgcc tcgatcgccg ccggcggcac gctcggcatc ttgattccgc cgtccaccat
     2161 catggtgatc tacggcatca tcaccgagac caacatcggg cgtctgttcg ccgccggcgt
     2221 cctgccgggc gtcctcgcgg tggcgctgct gtgcctcgcc gtgcgctggt cggtttggcg
     2281 cg
//
LOCUS       DBNHKE010000135         2915 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1080393, whole genome shotgun sequence.
ACCESSION   DBNHKE010000135 DBNHKE010000000
VERSION     DBNHKE010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1080393"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16O9_08950"
     CDS             <1..353
                     /locus_tag="AC16O9_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR6516783.1"
                     /translation="PASAPASAPAPEGATPAAAAAAAPGPAEAAPPPSSDEPYIETPR
                     CSSCDECIQINNKMFAYDANKQARIVDASAGTYRQLVEAAESCQVAIIHPGKPKNPDE
                     PGLEDLLQRAAAFP"
     gene            448..948
                     /locus_tag="AC16O9_08955"
     CDS             448..948
                     /locus_tag="AC16O9_08955"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR6516784.1"
                     /translation="MNIAPTVQHYLSAQQIEYEVIPHPRTVSSSGTAHAMHVPGTRLA
                     KAVVVKDNKGYVVAVLPASHHVALAQLGAALRRDALRLASEAELQDLFTDCALGAVPP
                     LGAAYGLPMIIDETLAEQPEVFFEAGDHEHVVHVTRNAFMRMTKNAPRAHFGEPGASG
                     GGLWMQ"
     gene            936..1127
                     /locus_tag="AC16O9_08960"
     CDS             936..1127
                     /locus_tag="AC16O9_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HIG1 domain-containing protein"
                     /protein_id="HIR6516785.1"
                     /translation="MDAMTVFVFIAAGLAALSLYKGVVSMAHGGEADQKASHKLMFQR
                     IAWQGAAVVFVLLAVLAAG"
     gene            complement(1147..1422)
                     /locus_tag="AC16O9_08965"
     CDS             complement(1147..1422)
                     /locus_tag="AC16O9_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6516786.1"
                     /translation="MPPKNEGDAAALASGNPVGYLIQVNNGPVIYHTGDTDVHNDTRL
                     ISQFYKVDVMFAAIGGHFTMDPVRAALDERGLGARMVEMKPGETRGF"
     gene            complement(1504..2913)
                     /locus_tag="AC16O9_08970"
     CDS             complement(1504..2913)
                     /locus_tag="AC16O9_08970"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6516787.1"
                     /translation="MDGTRAPRTTADVAVADGRIAAIGELTGASGDAEIDATGKIVAP
                     GFIDAHTHDDRLLLSAPDMAPKTSQGVTTVVAGNCGISLAPSPRGMKPVPPLDLLDAE
                     GGWYRFPGFADYVAELRAKPAAANAALLVGHSTLRVQTMDRLDRPANAREIERMRALA
                     DEALAAGAIGVSTGLWYEPASAATTEEVIEVCRPLAKRHGLYCTHMRDEGDRVLDSLE
                     ETFRIGRELGVPVVVSHHKVMGKANHGRSAETLPLIASRMRDQPIGLDCYPYCAASTI
                     LTASRAKIATRVLVTWSTPHPELAGMDLSEIEKKLGLATDEAIAKLLPAGAVYFAMDE
                     NDVQRILAFENTMVGSDGLPHDAAPHPRLWGTFPRVLGHYARGLGLFPLETAVYKMTG
                     LTAKTFGLEDRGVLREGYAADITVFDAGTVDEAATFARPIQPAKGIEAVIVNGEVVWK
                     EGKSTGARPGQVLLRSGLG"
BASE COUNT          438 a         1040 c          987 g          450 t
ORIGIN      
        1 cacccgcgtc cgcgcccgcg tccgcgcccg cgcccgaggg ggcgacgccc gccgcggccg
       61 ccgcggcggc gcctggtccc gccgaagcgg ccccgccacc gtcttccgac gagccctaca
      121 tcgagacgcc gcgctgctcg agctgcgacg agtgcatcca gatcaacaac aagatgttcg
      181 cctacgacgc caacaagcag gcacgcatcg tcgacgcgag cgccggcacc taccgccagc
      241 tcgtcgaagc cgcggagagc tgccaggtgg ccatcatcca tcccggcaag ccgaaaaacc
      301 ccgacgagcc ggggctggaa gatctgctgc agcgcgctgc cgccttccca taggcgccgc
      361 ggcggcgcaa ccttgacgta cgtcaaaacc cgtctcgccg atcgggtgta gcaatcaaga
      421 aagatcgagc agccgcagga ggcgaacatg aacatcgcac ccaccgttca gcactacttg
      481 tcggcgcagc agattgagta cgaagtcatc ccgcacccgc gcacggtgag cagcagcggc
      541 acggcgcacg ccatgcacgt gccgggcacg cgcctggcga aggccgttgt cgtcaaggac
      601 aacaagggct acgtggtggc ggtcctgccg gcaagccatc acgtcgccct ggcgcaactc
      661 ggcgcggcgc tgcgccgcga cgcgctgcgg ctggcgagcg aggcggagct gcaggatctg
      721 ttcaccgact gcgcgctcgg cgccgtgccg ccgctcggcg cggcctacgg cctgccgatg
      781 atcatcgacg agacgctcgc cgagcagccg gaagtgttct tcgaggctgg cgaccacgag
      841 catgtcgtgc acgtcacgcg caatgcgttc atgcgcatga ccaagaacgc accgcgcgcg
      901 catttcggcg agccgggcgc atcaggaggt ggcctatgga tgcaatgacg gtgttcgtct
      961 tcatcgcggc gggcctggcg gccctgtcgc tgtacaaggg cgtggtctcg atggcgcacg
     1021 gcggcgaagc cgaccagaag gccagccaca agctcatgtt ccagcgcatc gcctggcagg
     1081 gagcagcggt ggtattcgtg ctgctcgcgg tgctcgccgc cggctaggcg ccgccgcgcg
     1141 cggcgctcag aagccgcgcg tctctcccgg cttcatctcc accatgcgtg cgccgagccc
     1201 gcgctcgtcg agcgccgcgc gcaccgggtc catggtgaag tgcccgccga tcgccgcgaa
     1261 catcacgtcc accttgtaga actggctgat cagcctcgtg tcgttgtgca cgtcggtgtc
     1321 gccggtgtgg tagatcaccg ggccgttgtt cacctggatg agatacccca cggggttgcc
     1381 gctggcgagc gcagcggcgt cgccttcgtt cttcggcggc acgagctcgg agcagcgcgc
     1441 gcgccagcaa cttggtattt cgtcatggcg tgtcccctcc tttggtatgc gtcggccgct
     1501 acgttaaccc aatccgctgc gcaagagcac ctgcccgggc cgcgcaccgg tgctcttgcc
     1561 ttccttccag accacctcgc cgttcacgat caccgcctcg atccccttgg ctggctggat
     1621 cggccgcgcg aaggtcgccg cctcgtccac cgtgccggcg tcgaacaccg tgatgtccgc
     1681 ggcgtagccc tctctgagga ccccgcgatc ctccaggccg aaggtcttcg cggtgaggcc
     1741 cgtcatcttg tagaccgccg tctcgagcgg gaacaggccg aggcccctcg cgtagtgccc
     1801 gagcacgcgc gggaacgtgc cccacaggcg cgggtgcggc gcggcgtcgt gcggcaggcc
     1861 gtcggagccg accatggtgt tctcgaaggc gagaatgcgt tgcacgtcgt tctcgtccat
     1921 cgcgaagtac acggcgcccg cgggcagcag cttcgcgatc gcttcgtcgg tcgcaagccc
     1981 cagcttcttc tcgatctcgg aaaggtccat gcccgcgagc tcgggatgcg gcgtggacca
     2041 ggtgacgagc acgcgcgtgg cgatcttcgc gcggcttgcc gtgagaatgg tggaagccgc
     2101 gcagtacgga tagcagtcca gcccgatcgg ctggtcgcgc attctggatg cgatcagcgg
     2161 cagcgtctcc gccgagcgcc cgtgattggc cttgcccatc accttgtggt gcgacaccac
     2221 caccggcacg ccgagctcgc gcccgatgcg gaaggtctcc tccagcgaat ccaggacgcg
     2281 atcgccctcg tcgcgcatgt gcgtgcagta aaggccgtgg cgcttcgcga gcggccggca
     2341 cacctcgatc acttcttcgg tcgtcgccgc gctcgccggc tcgtaccaca ggccggtgga
     2401 cacgccgatc gcgcccgcgg cgagcgcctc gtcggcgagc gcgcgcatgc gctcgatctc
     2461 cctggcgttc gccggacgat cgaggcggtc catcgtctgc acgcgcagcg tcgagtggcc
     2521 gacgagcagc gcggcgttgg ccgccgcggg cttcgcgcgc agctccgcca cgtagtcggc
     2581 gaagcccggg aagcggtacc agccgccctc ggcgtcgagc aggtcgagcg gcggcacggg
     2641 cttcatgccg cgcggcgaag gcgcgagcga gatgccgcag ttgcccgcga cgacggtcgt
     2701 cacgccctgg cttgtcttcg gcgccatgtc gggcgcggaa agaagcagac ggtcatcgtg
     2761 cgtgtgcgcg tcgatgaaac cgggcgccac gatcttgccg gtggcgtcga tctcggcgtc
     2821 gccgcttgcg cccgtcagct cgccgatcgc cgcgatgcgc ccgtccgcga cggcgacgtc
     2881 ggcggtggtg cgcggcgcgc gcgtgccgtc gatca
//
LOCUS       DBNHKE010000136        17032 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_922588, whole genome shotgun sequence.
ACCESSION   DBNHKE010000136 DBNHKE010000000
VERSION     DBNHKE010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_922588"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC16O9_08975"
     CDS             complement(<1..448)
                     /locus_tag="AC16O9_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6516788.1"
                     /translation="MLAGCTPWPEEFARRYREQGYWEGITLFEMLRRSAAAAPTRIAV
                     VDGERRATCRELLAHAEQLAAGLWQLGLRPQERVLFQLGNGLQFLAAFLALLRIGAIP
                     VMVLPAHRRAEIAHFARHAQPVAHFVPARSDKFDYPALARAAAAECA"
     gene            complement(460..1890)
                     /locus_tag="AC16O9_08980"
     CDS             complement(460..1890)
                     /locus_tag="AC16O9_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6516789.1"
                     /translation="MKLFEPLDIRGMKVPNRIMVPAMVTHLCREDGVVTDGIVERYAR
                     YARGGAGLVVVEAMAVHQAKSGPLLRISDEAFVPGLRRIAEAVHACSDAKVVPQIIHF
                     LKVARSGWRQTVDMLAPAEIERIVGQFGDAVRRAREAGFDGAELHAAHAYTLASFLSR
                     RNSREDDYGRTLEGRMHLVGRVIAEARRKAGADFPIGIRINVEECIKDGYTVEEAKTI
                     AARLAELGVAYISLSAGGKFEDAVHVPGQVLFPYTGYSGDRAMPGAWFPAALNTGLAA
                     QVKAHLTARGFATPLAVAGKLSAPGDAERVLAEGAADMVAIARALLADPDWPVKVKHG
                     HAERIVQCDYCNVCKNLDGTHNPVVCALWPQRAVQAPPYDPQAAVPVWGADQGALAAS
                     LGREGIALEWRKAAGADRYEIYRADADGAPRLLDAQRAVRWVDPEILGGRRYRYHVRP
                     CAAGGRAGPPSNTVVVDVPAPAYLRA"
     gene            complement(2021..3628)
                     /locus_tag="AC16O9_08985"
     CDS             complement(2021..3628)
                     /locus_tag="AC16O9_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516790.1"
                     /translation="MSARAARICREALLVAVVLAGLAPAAPAREARAANPTLVRADAA
                     FEAGERDLARRLYRSVLASDPDNSRATFQLAKLAPRGSAEALALLRRYVALEPSDPWG
                     TMALGDDLARAGRVEAAIEQYALARRRAPTEPDVYAGFGRILRDAGRIDEAVDSFERW
                     VSLRPGDPSAWFELGRARERAARHPEAARAYARALRIKTDERTEKRLEDALAESSPSL
                     RPLFGSSDDSDGNRVERLGFEAEWPFTDRSRLGVRWDRYEVSDALAEGTASALALYAK
                     WRPLAALAVEGSAGTARLRAPGSAGASHTLLGLRIRGKQSAQGLSGELRMARNPLIAT
                     PGLIAQPVQLSEVKGTAEVPLFGPFRARVRGQRGELESPVDVNHRSGYQVGPVYQWRP
                     ATEFGAFYGEQGYDHPSTAGYFAPRRVQLLELGTYLEYEGLWPLSLALDAGAGRQRVA
                     RHGEPVGEWTTTYRLWALASWTLQPGVRLELELEHYDSPLAGVGVSSAAEWRYDSVTL
                     SLRFGVQREHARAYLARGADRPPAAGR"
     gene            complement(3625..3999)
                     /locus_tag="AC16O9_08990"
     CDS             complement(3625..3999)
                     /locus_tag="AC16O9_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516791.1"
                     /translation="MTPGLPGTGIGGLFYVLSALLMPICELWRRWRGEAGGRGALVAR
                     QFAIAVGAVAAMSLAFWLLDSLLPANPAGARAAGATFLSWTLPIRVSALLVTSAVLAA
                     VLGAVHLLRLWLRIRAPRTAVR"
     gene            complement(4004..5023)
                     /locus_tag="AC16O9_08995"
     CDS             complement(4004..5023)
                     /locus_tag="AC16O9_08995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6516792.1"
                     /translation="MELTVIIPAYNEAEFVGDTIRSLREQSVRPEHIILVDDGSTDGT
                     GEVGRRAGVTVIRPPRNTGSKAGAQSFALPWVRTELVMAIDADTTLDPSAVERLMKCF
                     TRPDVGAACGFVLPRRTRTLWERGRFIEYLFAFTFYKQIQDYYHRPLISSGCFSMYRT
                     ELLRRHGGWSGRTLAEDMDLTWSFHQAGTGVRFVPEAVCYPIEPYNFRLMRKQLQRWS
                     HGFVQNVALHWRGVLAVPFLRSAVAVALWDAIVASLAFLVAVPVLAIALGSPWPLLAY
                     FIDAPAILVPLLVGAIPRRETARTLASLPSFFVLRLVNGFFFLEAVVTEWLLRRPYHV
                     YEKGH"
     gene            complement(5255..6793)
                     /locus_tag="AC16O9_09000"
     CDS             complement(5255..6793)
                     /locus_tag="AC16O9_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR6516793.1"
                     /translation="MYELLWHALVGLFQFKYLMPLVIGTVAGVVGGALPGVTITMTVI
                     IVLPFTFGLDPLQGLAAMTGVYVGGSAGGLVTATLLGIPGTPSSVATTFDGFPMARKG
                     EAGRALWLGVWGSFLGGMIGGVFLIAATEPLAKFALEFGPWEFFSLFVLALSMVAGLV
                     GRSLLKGFLSGAIGLVVTVLGNDPVMGQPRLTLGIEFLEGGVPFLPVLIGVFAFAQIM
                     SDVEKMGAAARAGAALERAVSLAVSHLRVLGEILRRPFLLAWSSLVGVVIGVLPAIGG
                     SAANIMAYDQAKKFSRHPERFGTGTPEGIIASESANNANVGGSLVTIMAFGIPGDAVT
                     AVMLGALTIHGIQSGPLFISQNTGVAYGIFAAYLLAHPIMAIVIAIGARFMLRVVTVP
                     KAALFPVVLVLCTIGAYALANAMSAVYILLAFGLLGYAMVKVGLPLAPLILGVILGDQ
                     IETNLIRALMTDENLWLFFTRPISGVLLALSAGSVVLALWQHRRQARRERREAPGAGG
                     EADF"
     gene            complement(6797..7261)
                     /locus_tag="AC16O9_09005"
     CDS             complement(6797..7261)
                     /locus_tag="AC16O9_09005"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR6516794.1"
                     /translation="MIGRDGLTGLAAAAASLFLFALTLGLERNPLVPIGPGFYPRIVL
                     GITAALGLALLVQDLLRARVAAPARAPINTRLVVLTFSAFAAYVLLLPLLGFRVATFV
                     YVLGATFLLEPPRRAGLWARGVVLAALTTAATYYVFEQYLAVLLPRGTWTGY"
     gene            complement(7284..8222)
                     /locus_tag="AC16O9_09010"
     CDS             complement(7284..8222)
                     /locus_tag="AC16O9_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6516795.1"
                     /translation="MKKLVAALAVSLFPALSLAAFPDRPIKLIVPWGAGGDTDVIYRT
                     FAPLMQKYLGGTIVVANVGGASGTKGAKEAKDSPADGYTIFAIHDSIHSTYYTGVADV
                     NYTDFEPLCLVSSTPSIVTASPKTPWNDMKSLLADAKARPGQITVGATLGSTSHFFPA
                     LVAKAAGIQFKYVSYEGTAPRMNALLGGHIDLAESNLTQKGKADAGQLKFLAIASNAR
                     HPELPSVPTLKELGVDVEYAVNRGLVAPKGTPADVLAKLRSACGAAARETGLADALKK
                     HGTLVRYLDEIAYARFFQTNDAESKEVAKDLGMLKR"
     gene            8257..8700
                     /locus_tag="AC16O9_09015"
     CDS             8257..8700
                     /locus_tag="AC16O9_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6516796.1"
                     /translation="MFPGKWYDEVAVGERFGTSLTVTEAHLVLGSGMFGDFNPLHVNE
                     EFARKSMFGTRILHGPFTSALVSAPVGNYFAGTALAYLEHNCRFKAAVRPGDTLSSEW
                     TIAEKLDKPKHKGGIVVLRCKCTNQTGELAVEAEGKLLVASRPRA"
     gene            complement(8792..10426)
                     /locus_tag="AC16O9_09020"
     CDS             complement(8792..10426)
                     /locus_tag="AC16O9_09020"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR6516797.1"
                     /translation="MPDRYDELARDFRWNVPPRYNIARACCGQWAADRTRFALYWEDE
                     SGATAAYSFWDIQQQANRLSNALAGLGVRRGDRVALLLPQRPESAIAYMAVFQMGAVA
                     LPLSHLFGPDALEYRMEHAGASVVIVEPTTIANLWAVRGKLSQLRHVIGVGGARESGV
                     HSWEALLQKASADFAPLDTAADDPALIIYTSGTTGAPKGAYEAHRLLVGNLSGFVHSH
                     DFFPQPGDMFWSPADWAWAGGLFDALLPSWACGIPILGYRGKFDAEKAYDLLARYGVR
                     NSFLFPTALKLMMKAVPEPRSKYRLNLRSIMSAGESVGVTVIEWAKEHLGVTINEMFG
                     QTEINYVVGNCQAAWPVKPGSIGRPYPGHRVAVIDGEGREVPRGALGEIAVAKTCNGE
                     RDPVHFLEYWKNPQATKEKYVGEWGLTGDQGKMDEDGYLWYQGRSDDVIKSAGYRIGP
                     AEIENCLVKHPAVANAAVIGKPDATRGAIVKAFIVLQPGQAPSKALEDDIQKHVRARL
                     APYEYPREIEFIDALPMTTTGKVQRKELRKREEARG"
     gene            10450..11103
                     /locus_tag="AC16O9_09025"
     CDS             10450..11103
                     /locus_tag="AC16O9_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR6516798.1"
                     /translation="MNSQALVFDAYGTLYDVHTVIRRCDALWPGKGTALSQLWRQKQL
                     EYTWQRSLMRRYVPFSQITREALAYACESLKLALDAAKADSLMQEYLRLSPYPDVPDA
                     LTKLKGRKAILTNGSPDMIEPLVQHSGLKFDAVLSVDAVKQFKPVPEVYELAVARLGV
                     AKDAIGFVSSNCWDALGAKSFGFRVYWINRTGAPVDRLGFQPDALLGSLGDLPEVLR"
     gene            11087..11620
                     /locus_tag="AC16O9_09030"
     CDS             11087..11620
                     /locus_tag="AC16O9_09030"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6516799.1"
                     /translation="MKFCANCGAPVARRVPPGDSLPRYVCDACGEIHYQNPKLVVGAL
                     AEHEGRVLLCRRAIEPRYGYWTLPAGFMENDETAAQAALRETLEEAGARIELAAPFSM
                     ISVPRVNQVHLFYRARVLDLEFKPGEESLEVALVDEAKVPWKELAFRTVAITLRHWFA
                     DRARGTFGFHAEDLPLR"
     gene            11647..12930
                     /locus_tag="AC16O9_09035"
     CDS             11647..12930
                     /locus_tag="AC16O9_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6516800.1"
                     /translation="MRKALLFPLLAFAPLAAAAEAKLDPANTAWMITASVLVLFMTLP
                     GLALFYAGLVRAKNVLSVLMQCFAIACSATLVWVAVGYSLAFSDGGAANAWIGGLDKA
                     FLLGVDSKSLSGSIPETVFAMFQLTFAIITPALVVGAYAERVRFGGMLLFSMLWLLVV
                     YVPVAHWVWGGGWLQQLGVMDFAGGLVVHLNAGLAALVCALVLGVRRGFPETAMPPHN
                     MTMAVTGACLLWVGWFGFNAGSALAADGTAGMAMLVTHVGAATGSLAWLVCEWLRYGK
                     PSVLGIVTGMVAGLGTITPASGFVGPLGAIAIGAAAGVVCFAATNYMKRSLGVDDSLD
                     VFPVHGVGGILGTILTGVFVAAALGGVGLPDGRSMGGQVLIQLGGVLAIAAWCGVATW
                     ILLKVTQAAAGLRVGSEGETEGLDTHLHNEKGYNL"
     gene            12947..13642
                     /locus_tag="AC16O9_09040"
     CDS             12947..13642
                     /locus_tag="AC16O9_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR6516801.1"
                     /translation="MERRDFIKFCAASAAAAGAPAVSADARPHFYERVKLVGEDRAPL
                     RARDVPANRNLIFHYPYATTPCFLLNLGRPVQGVALLKTENNQPYEWRGGVGAGRSIV
                     AYSAICAHKLTYPTRDISFISFRAEKTTGNKFANVIHCCSEHSQYDPAAGGRVLAGPA
                     PQPLAAILLEHDAATDDLYAVGTLGGEMFEQFFAKYEFRLALENGGALRRTVNGVSPV
                     TPLERYCKQQVKC"
     gene            13636..14343
                     /gene="aat"
                     /locus_tag="AC16O9_09045"
     CDS             13636..14343
                     /gene="aat"
                     /locus_tag="AC16O9_09045"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR6516802.1"
                     /translation="MLKWLGPGDPFPPVQSALQDPAGLLCAGGDLSAERLLEGYRRGI
                     FPWYSGDEPILWWSPDPRMVLYCDELRVSRSLSKSLRNKGYEVRIDTAFRQVLAGCAG
                     PRADSGGTWLGEDMRIAYARLHRAGHAHSFETWRGGELVGGLYGVALGRMFFGESMFS
                     RATDASKVALVGLVGELGRRGFPLIDCQVRTPLLAALGAREIPRRSFLRELAALVHYD
                     EPPGKWTRRTEPDVPAE"
     gene            14327..15076
                     /locus_tag="AC16O9_09050"
     CDS             14327..15076
                     /locus_tag="AC16O9_09050"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR6516803.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLITTD
                     LYGDLVKSGFRRSGIFTYRPHCDSCRACVPVRIPASEFVASRSQRRAWREHAGLTAQV
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLVEFRDQGQLVM
                     VSIVDYLPDGLSSVYTFFEPERRGASYGTYNVMWQIDACRALGMPYLYLGYWIRESPK
                     MAYKSRFLPIEGFSGGGWRRLSESDLARMRF"
     gene            15080..16120
                     /locus_tag="AC16O9_09055"
     CDS             15080..16120
                     /locus_tag="AC16O9_09055"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR6516804.1"
                     /translation="MLYALVRPLLFRLDPELAHELTLGLADAPLRFGLMRVARAPGAP
                     VRAMGLEFPNAVGLAAGLDKNAEYVDALSRLGFGFLEVGTVTPRPQPGNPRPRLFRLP
                     RAEALINRMGFNNVGLDAFLDNLSRVRWRGILGINIGKNADTPIERAADDYAACFERV
                     YARASYVTVNVSSPNTKNLRDLQENRQLDALLARLAGLREPLAQRHGKRVPLALKVAP
                     DLEGPQVQAIADAVRRHGVDAVIATNTSVSREGVDGLRHAGEAGGLSGAPIRGRATAT
                     LAALAAALQGEAALVGVGGILRGEHAVEKLDAGASLVQLYTGLIYRGPDLVAECVSAC
                     RKKSRTASSASR"
     gene            16066..>17032
                     /locus_tag="AC16O9_09060"
     CDS             16066..>17032
                     /locus_tag="AC16O9_09060"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6516805.1"
                     /translation="MRIGLPKEIKDGEQRVALTPAGAAALERAGHEVVFEPGAGAGVG
                     FGDEAYAGAGAAPGDAWACELVVKVKELQPAEYARPRRGQTVFGFQHFGPEPALLEAA
                     LASGASFVAFETVGQASGLPILAPMSAIAGRLAVQVGAWCLQKQNGGSGVLLPGLDGI
                     APGKVVILGAGNVGANALAVACGIGASVSVFARSEKRFAALRQRYPDARLSTSAPDIA
                     DADLVIAGVLTPGQMSPKLISRTMLRAMRPGSALVDVGIDQGGVAETSRPTKHSAPIY
                     LEEGIVHYCVPNMPAACARTASLALERALLPYALKLAAGETDADLD"
BASE COUNT         2592 a         5996 c         5972 g         2472 t
ORIGIN      
        1 aggcgcactc ggccgccgcc gcgcgcgcga gcgccgggta gtcgaacttg tcggagcgcg
       61 cgggcacgaa gtgcgcgacc ggctgcgcgt gccgggcgaa gtgcgcgatc tcggcccgcc
      121 ggtgcgcggg cagcaccatc accggtatcg cgccgatgcg cagcagcgcg aggaacgcgg
      181 cgagaaactg caggccgtta ccgagctgga agagcacgcg ctcctgcggc cgcagcccga
      241 gctgccacag gccggcggcg agctgctccg catgcgcgag aagttctcgg cacgtcgcgc
      301 ggcgctcgcc gtcgacgacc gcgatgcgcg tcggcgcggc cgccgcggag cgccgcagca
      361 tctcgaagag cgtgatccct tcccagtaac cctgctcgcg gtagcggcgc gcgaactcct
      421 cgggccaggg cgtgcagccg gcgagcatgc gcgcgcgcgt caggctcgca ggtaggcggg
      481 cgcgggcacg tcgacgacca cggtgttcga cggcggcccg gcgcggccgc ccgcggcgca
      541 cgggcgcacg tggtagcggt agcgcctgcc gccgagaatc tccggatcga cccagcgcac
      601 ggcccgctgc gcgtcgagga gccgcggcgc gccgtcggca tcggcgcggt agatctcgta
      661 gcggtcggcg ccggcggcct tgcgccactc gagcgcgatg ccctcgcggc cgagcgacgc
      721 ggcgagcgcg ccctggtccg cgccccagac cggcaccgcg gcctgcggat cgtacggcgg
      781 cgcctgcacg gcgcgctggg gccagagcgc gcagaccacc gggttatgcg tgccgtcgag
      841 gttcttgcag acgttgcagt agtcgcattg cacgatgcgc tcggcgtggc cgtgcttcac
      901 ctttacaggc cagtcggggt cggcgagcag cgcgcgcgcg atggcgacca tgtcggcggc
      961 gccctcggcg agcacgcgct cggcgtcgcc gggcgcggac agcttgcccg cgacggcgag
     1021 gggcgtggcg aatccccggg ccgtcaggtg tgccttcacc tgcgcggcga ggcccgtgtt
     1081 gagcgcggcg ggaaaccagg caccgggcat cgcgcggtcg ccggagtaac cggtgtaggg
     1141 gaacagcacc tgtcccggga cgtgcaccgc atcctcgaac ttgccgccgg cggagagcga
     1201 tatgtacgcc acgccgagct cggccaggcg cgccgcgatg gtcttcgctt cctcgaccgt
     1261 gtagccgtcc ttgatgcact cctcgacgtt gatgcggatg ccgatgggga agtcggcgcc
     1321 ggccttgcga cgggcttcgg cgatcacgcg gccgacgaga tgcatccggc cctcgagcgt
     1381 gcggccgtag tcgtcctcgc gggaattgcg gcgcgagagg aaagaggcga gcgtgtacgc
     1441 gtgcgccgcg tgcagctcgg cgccgtcgaa gcccgcttcg cgcgcgcggc gcaccgcgtc
     1501 gccgaactgc ccgacgatgc gctcgatctc cgccggcgcg agcatgtcga cggtctggcg
     1561 ccagcccgag cgcgcgacct tgagaaagtg gatgatctgc ggcacgacct tcgcgtccga
     1621 gcacgcgtgc accgcctcgg cgatccggcg cagccccggc acgaaggcct cgtcgctgat
     1681 gcgcagcagc gggccggatt tcgcctggtg caccgccatc gcctcgacga ccaccagccc
     1741 cgccccgccg cgcgcgtagc gcgcatagcg ctcgacgatg ccgtcggtga cgacgccgtc
     1801 ctcccggcaa agatgcgtca ccattgccgg caccatgatc cggttcggga ccttcatgcc
     1861 gcggatgtcg agcggctcga acagcttcat cgtgcgcctc cccagtccgc gatcgtgcgg
     1921 cggaggcgtc gccgcgcctt gatccgcatc aagtgcgctg gcggcgccgc gcacgcggcc
     1981 gagcacatga tcgaggcggt gaagtagcaa acagcgcgct ctagcgcccg gccgccggcg
     2041 gccggtcggc ccctcgggcg agataggcac gggcgtgctc gcgctgcacg ccgaaacgca
     2101 gcgacagcgt gaccgaatcg tagcgccact cggcggccga cgacacgccg acgcccgcga
     2161 gcggcgagtc gtagtgctcg agctcgagct ccaggcgcac acccggctgg agcgtccacg
     2221 aggccagcgc ccagagccgg taggtcgtgg tccactcgcc cacgggctcg ccgtgtcgcg
     2281 cgacgcgctg gcgtccggcg cccgcgtcca gggcgaggct gagcggccag agaccttcgt
     2341 actccaggta ggttccaagc tcgagcagct ggacgcggcg cggcgcgaag tagcctgccg
     2401 tggaagggtg gtcgtagccc tgctcgccgt agaacgcgcc gaactccgtc gcgggccgcc
     2461 actggtagac cggcccgacc tggtagccgg agcggtggtt gacgtcgaca ggcgactcga
     2521 gctcgccgcg ctgaccgcgc acgcgggcgc gaaaaggccc aaacagcggg acctctgcgg
     2581 tgcccttcac ctccgagagc tgcacgggct gtgcgatcag ccccggcgtc gcgatcagcg
     2641 gattgcgcgc catgcgcagc tctcccgaca aaccctgcgc ggactgcttt cctcgaatgc
     2701 gcagtcccag cagcgtgtgg ctcgcgcccg ccgatcccgg cgcgcgcagc cgcgcggtgc
     2761 cggccgagcc ctccacggcg agggcggcga gcggccgcca cttcgcgtag agcgcgagcg
     2821 cgctggcggt cccctcggcg agcgcgtcgc tcacctcgta gcggtcccag cgcacgccca
     2881 gccgcgagcg gtccgtgaac ggccactcgg cctcgaagcc caggcgctct acgcggttgc
     2941 cgtccgaatc gtcgctgctg ccgaacagcg gccgcaacga gggtgaggat tccgcgagcg
     3001 cgtcctcgag ccgcttctcg gtgcgctcgt ccgtcttgat ccgcagggcg cgggcgtagg
     3061 cccgcgcggc ttcgggatgc cgcgctgcgc gctcgcgcgc ccgcccgagc tcgaaccagg
     3121 ccgaggggtc cccgggacga agcgaaaccc atcgctcgaa gctgtccacg gcttcgtcga
     3181 tccgccccgc atcgcgaaga atccggccaa accccgcgta tacgtcgggc tcggtgggcg
     3241 cacgccgacg cgcgagcgcg tattgctcga tcgccgcctc gacacggcct gcccgggcaa
     3301 ggtcgtcgcc gagcgccatg gtgccccagg gatccgaggg ctcgagggcc acgtagcgtc
     3361 gcaggagcgc gagcgcttcc gccgagccgc gcggtgcgag ctttgccagc tggaacgtcg
     3421 cgcgggaatt gtcagggtcg gaggcgagca ctgaccggta gaggcgccgc gcgaggtccc
     3481 gctcgcccgc ctcgaacgcg gcgtccgccc gcaccagtgt cggattcgcc gcacgcgcct
     3541 cccgcgccgg cgcggcggga gcaaggccgg caaggacgac cgcgacgagt agcgcttcgc
     3601 ggcagatgcg cgccgcgcgc gcgctcatcg cacggcggtt cgcggcgcgc ggatcctcag
     3661 ccacaggcgc aggaggtgca ccgcgccgag cacggcggcg agcacggcgg aggtcaccag
     3721 cagcgccgag acgcgaatcg gcagcgtcca ggacaggaat gtcgcgcccg ctgcgcgcgc
     3781 gccggccgga ttcgccggca gcagcgaatc gagcagccag aaggcgagcg acatcgcggc
     3841 gaccgcgccg acggcgatcg cgaactgccg ggcgaccagc gcgccgcggc ccccggcctc
     3901 gccacgccag cgccgccaca gctcgcagat cggcatcagg agcgcgctga gcacgtagaa
     3961 cagcccgccg atgcccgttc cggggagtcc aggggtcatg gatctagtgc cctttctcgt
     4021 aaacgtggta cggccgtcgc aacagccact cggtgacgac ggcttccagg aagaagaacc
     4081 cgttcaccag ccgcagcacg aagaacgacg gcaggctcgc cagcgtgcgg gcggtctcgc
     4141 ggcggggaat cgcgccgacg aggagcggca ccaggatggc gggcgcgtcg atgaaatacg
     4201 cgagcagggg ccagggactg cccagcgcga tcgccagcac cggcacggcc acgaggaacg
     4261 cgagcgaggc gacgatcgcg tcccacaggg cgaccgccac cgccgagcgc aggaacggca
     4321 cggcgagcac cccgcgccag tgcagggcca cgttctgcac gaagccgtgg ctccagcgct
     4381 gcagctgctt gcgcatcagg cggaagttgt atggctcgat gggatagcac accgcttcgg
     4441 gcacgaagcg caccccggtg ccggcctggt ggaagctcca ggtcaggtcc atgtcctcgg
     4501 ccagcgtgcg gcccgaccat ccgccgtgcc ggcgcagcag ctcggtgcgg tacatcgaga
     4561 agcaccctga ggagatgagc ggccggtggt agtagtcctg gatctgcttg tagaacgtga
     4621 acgcgaagag atactcgatg aaccgtcccc gctcccacag cgtgcgcgtc cggcgcggca
     4681 gcacgaagcc gcacgccgcg cccacgtcgg gtcgggtgaa gcacttcatg agccgctcca
     4741 cggcgctcgg atcgagggtg gtgtcggcat cgatcgccat caccagctcg gtccgcaccc
     4801 acggcagcgc gaacgactgc gcgcccgcct tggagcccgt gttgcgcggg ggccgaatga
     4861 ccgtaacgcc cgcgcggcgc ccaacctcgc ccgtgccgtc ggtcgaccca tcgtcgacca
     4921 ggatgatgtg ctcagggcga acagactgct cgcgcaggct gcggatcgtg tcgcctacga
     4981 actcggcctc gttgtaggcc gggatgatga ctgtcaactc cattgccggg gcgtgcggtc
     5041 caaggcgaga gaaacttcgt gggaaatttg acccaaactt tggcgatgtt agcggtaacg
     5101 agacagacag acaagggtct tttcctataa atcattgaaa ttaatgatgt cctgtccccg
     5161 ccaggcgaca gtggggaggc tccgcagact ccgggagccc gctgatcggc gggcgaaggc
     5221 ttcaggcgcg ctgcggcgcc aagaagtaca gccgctagaa atccgcttcc ccgccggcgc
     5281 ccggcgcttc gcgcctttcg cgccgcgcct gccgccggtg ctgccacagc gccagcacca
     5341 ccgagcccgc cgacagcgcg agcagcacac ccgagatcgg ccgcgtaaag aacagccaca
     5401 ggttctcgtc cgtcatcagc gcccggatca ggttggtttc gatctggtcg ccgaggatca
     5461 cgccgaggat cagcggcgcg agcggcagcc cgaccttcac catggcgtag ccgagcagcc
     5521 cgaaggcgag caggatgtac acggccgaca tggcgttggc gagcgcatag gcaccgatcg
     5581 tgcacaggac cagcaccacc gggaacagcg ccgccttggg caccgtcacc acgcgcagca
     5641 tgaagcgcgc gccgatcgcg atcacgatcg ccatgatcgg atgcgcgagc aggtaggcgg
     5701 cgaagatgcc gtaggcgaca cccgtattct gcgagatgaa caacggcccg gactggatgc
     5761 cgtggatggt gagcgcgccc agcatcacgg cggtgacggc gtcgcccggg atgccgaagg
     5821 ccatgatggt gaccagcgaa ccgccgacat tggcgttgtt cgccgactcc gaggcgatga
     5881 tgccctcggg cgtgcccgta ccgaagcgct cgggatggcg cgagaacttc ttcgcctggt
     5941 cgtaggccat gatgttggcg gcgctgccgc cgatcgccgg cagcacgccg atgaccacgc
     6001 cgaccaacga cgaccacgcg agcaggaacg gccgccgcag gatttctccc agcaccctca
     6061 ggtgcgacac cgccaggctc accgcgcgct cgagcgccgc gccggcgcga gccgcggcgc
     6121 ccatcttctc gacgtcggac atgatctgcg cgaaagcgaa cacgccgatc agcaccggga
     6181 ggaacggcac gccgccttcc aggaactcga tgccgagcgt gagccgcggc tggcccatca
     6241 ccgggtcatt cccgagcacg gtcaccacca ggccgatcgc accggaaagg aagcccttca
     6301 gcagcgagcg gcccaccagg cccgccacca tcgacagcgc gagcacgaac agcgaaaaga
     6361 actcccaggg cccgaactcg agcgcgaact tcgccagcgg ctcggtggcg gcgatcagga
     6421 acacgccgcc gatcatgccg ccgaggaacg agccccacac gccgagccag agcgcgcgcc
     6481 cggcctcgcc cttccgcgcc atcgggaagc cgtcgaacgt ggtcgccacc gacgaaggcg
     6541 tcccggggat gccgagcagc gtggcggtga ccagtccgcc cgccgagccg ccgacgtaca
     6601 cgccggtcat cgccgcgagg ccctgcagcg ggtcgaggcc gaaggtgaac ggcagcacga
     6661 tgatcaccgt catggtgatg gtcaccccgg gcagcgcccc gcccacgacg cccgcgacgg
     6721 tgccgatcac gagcggcatc aggtacttga actggaacag gccgacgagc gcgtgccaca
     6781 gcagctcgta cacggctcag taccccgtcc aggtgccgcg cggcagcagc accgcgaggt
     6841 actgctcgaa cacgtagtac gtcgcggcgg tggtcagcgc ggcgagcacg acgccgcgcg
     6901 cccagaggcc ggcgcgccgc ggcggctcga gcaggaacgt ggcgccgagg acgtatacga
     6961 acgtcgccac ccgaaagccg agcagcggca gcaacagcac gtacgcagcg aaggcgctga
     7021 aggtcagcac caccaggcgt gtgttgatgg gcgcccgggc cggcgccgcg acgcgtgcgc
     7081 gcagcaggtc ctgcacgagc agcgcaaggc cgagcgccgc ggtgatgccc agcacgatgc
     7141 gcggatagaa gccgggcccg atcggcacca gcggattgcg ctcgaggccg agcgtgagcg
     7201 caaaaagaaa gaggctcgcc gcagctgcgg cgagccccgt caggccgtca cggccgatca
     7261 tgccgggcgg acgaagcgcg cgctcagcgc ttcagcatgc cgaggtcctt ggcgacctcc
     7321 ttgctctccg cgtcgttggt ctggaagaag cgcgcgtagg cgatctcgtc caggtagcgc
     7381 accagcgtgc cgtgcttctt cagcgcatcg gcgagtccgg tctcgcgggc cgccgcgccg
     7441 caggccgagc gcagcttcgc cagcacgtcg gcaggcgtgc ccttgggcgc caccaggccg
     7501 cggttcaccg cgtactccac gtcgacgccc agttccttca gcgtcggcac gcttggcagc
     7561 tccgggtggc gcgcgttgct cgcgatggcg aggaacttga gctggccggc gtcggccttg
     7621 cccttttgcg tcaggttcga ttccgccagg tcgatgtgcc cgcccagcag cgcgttcatg
     7681 cgcggcgccg tgccctcgta ggacacgtac ttgaactgga tgccggcggc tttggccacg
     7741 agcgcgggga agaagtggct ggtcgagccg agcgtcgcgc ccaccgtgat ctgccccggg
     7801 cgcgccttgg cgtcggcgag gagcgacttc atgtcgttcc agggtgtctt cgggctggcg
     7861 gtgacgatcg agggcgtcga gctcaccagg cacagcggct cgaagtccgt gtagttcacg
     7921 tcggccacgc ccgtgtagta cgtggagtgg atcgagtcgt ggatggcgaa gatggtgtag
     7981 ccgtcggccg gcgaatcctt ggcctccttc gcacccttgg tgccggaggc gccgcccacg
     8041 ttggcgacga cgatggtgcc gccgaggtac ttctgcatca gcggcgcgaa cgtgcggtag
     8101 atgacgtcgg tgtcgccgcc cgcgccccag ggcacgatca gcttgatcgg ccggtccggg
     8161 aaggcagcga gcgaaagcgc ggggaacagc gaaacggcga gtgcggcaac gagttttttc
     8221 atggtctctc ctgcagagga agccgcataa taagtcatgt tcccgggaaa atggtacgac
     8281 gaggtggccg tcggcgagcg cttcggcacc agcctcacgg tcaccgaagc gcacctggtg
     8341 ctcggctccg gcatgttcgg cgacttcaac cccttgcacg tcaacgagga attcgccagg
     8401 aagtcgatgt tcggcacgcg catcctgcac gggccgttta ccagcgcgct cgtctcggcg
     8461 ccggtcggca attactttgc gggcaccgcc ctcgcctacc tggagcacaa ttgccgcttc
     8521 aaggcggcgg tgcggcccgg ggatacgctg agctcggagt ggaccatcgc cgagaagctc
     8581 gacaagccca agcacaaggg cggcatcgtg gtgctgcgct gcaagtgcac gaaccagacg
     8641 ggcgagctcg ccgtcgaggc cgagggcaag ctgctggtgg cgagccggcc tcgcgcctga
     8701 acggcccgac ccagcgccgg tcgaagcgca tctcgtcccg cgcgcagcac ccgggcgtgc
     8761 gcgtgacctc cgcctgcaag cagtgtccgc ttcaaccgcg cgcttcttcg cgcttgcgca
     8821 gttccttgcg ctgcaccttg ccggtggtgg tcatgggcag cgcgtcgatg aactcgatct
     8881 cccttggata ctcgtagggc gcgaggcgtg cgcgaacgtg cttctggata tcgtcctcca
     8941 gcgccttgga gggcgcctgc ccgggttgca gcacgatgaa cgccttgacg atcgcgccgc
     9001 gcgttgcgtc gggcttgccg atcaccgcgg cattggcgac cgcagggtgc ttgacgaggc
     9061 agttctcgat ctccgccggg ccgatgcggt agcccgcgct cttgatgaca tcgtccgagc
     9121 gcccctggta ccagaggtaa ccatcctcgt ccatcttgcc ctggtcgccg gtcaggcccc
     9181 actcgccgac gtacttctct ttcgtcgcct gaggattctt ccagtattcg aggaaatgca
     9241 ccggatcgcg ctcgccgttg caggtcttcg ccaccgcgat ctcgcccagt gcgccgcgcg
     9301 gcacttcgcg gccctcgccg tcgatcaccg ccacgcggtg ccccggatag ggccggccga
     9361 tggagccggg cttcaccggc caggccgcct ggcaattgcc gaccacgtag ttgatctcgg
     9421 tctggccgaa catttcgttg atggtgacgc cgagatgctc cttcgcccac tcgatcacgg
     9481 tgacccccac gctctcgccc gcgctcatga tcgagcgcag gttgaggcga tatttcgagc
     9541 gcggctcagg caccgccttc atcatcagtt tcagggccgt ggggaacagg aacgaattgc
     9601 gcacgccgta gcgcgccagc aggtcgtagg ccttctcggc gtcgaacttg ccgcggtacc
     9661 cgaggatcgg gatgccgcac gcccacgagg gcagcaacgc atcgaacagg ccgccggccc
     9721 aggcccagtc cgcgggcgac cagaacatgt ccccgggctg cggaaagaag tcgtgcgaat
     9781 gcacgaagcc gctcaggttg ccgacgagca ggcggtgtgc ctcgtaggcg cccttgggcg
     9841 cgccggtggt gccgcttgtg tagatgatga gtgccgggtc gtcggccgcc gtatcgagcg
     9901 gcgcgaaatc cgcactcgcc ttctgcagca gcgcctccca ggagtgcacc cccgactcgc
     9961 gcgcaccgcc cacgccgatc acgtggcgca attgcgagag cttgccgcgc accgcccaga
    10021 ggttggcgat cgtcgtcggc tcgacgataa cgacgctcgc gccggcgtgc tccatgcggt
    10081 attccagggc gtccggcccg aacaagtgcg atagcggcag cgccaccgcg cccatctgga
    10141 agaccgccat gtacgcgatg gcggattccg ggcgctgtgg cagcagcagc gcaacgcggt
    10201 cgccgcgccg cacgccgagg ccggcaaggg cgttcgacaa gcggttggcc tgctgctgta
    10261 tgtcccagaa gctgtaggcc gccgtcgccc ccgactcgtc ctcccagtac agggcgaaac
    10321 gcgtgcggtc cgccgcccac tgcccgcagc aggcccgcgc gatgttgtag cgcggcggaa
    10381 cgttccagcg gaaatcgcgg gccagctcgt cgtaccggtc gggcatcgcg ggcattctat
    10441 acttcgcaca tgaactccca agccctggtg tttgatgctt acggcacgct ctacgacgtg
    10501 cacacggtga tccgccgctg cgacgcgctc tggccgggca agggcaccgc gctgtcgcag
    10561 ctgtggcggc agaagcagct cgagtacacc tggcagcgaa gcctgatgcg gcgatacgtg
    10621 ccgttctcgc agatcacgcg ggaagccctc gcctacgcct gcgagtcgct caagctggcg
    10681 ctcgatgccg cgaaagcgga ctcgctgatg caggagtact tgaggctctc gccctacccc
    10741 gacgtccccg acgcgctgac caagctgaaa ggcaggaagg ccatactcac caacggctcg
    10801 cccgacatga tcgagccgct ggtgcagcac tcgggattga agttcgacgc cgtgctgagc
    10861 gtggacgccg tcaagcagtt caagccggtg cccgaggtgt acgagctcgc cgtcgcgcgc
    10921 ctcggcgtgg cgaaggacgc gatcggcttc gtctcgtcca actgctggga cgcgctcggc
    10981 gccaagtcct tcggcttccg ggtgtactgg atcaaccgca cgggcgcgcc ggtggaccgg
    11041 ctcggctttc agcccgacgc gctgctcggc tcgctcggag acctgcctga agttctgcgc
    11101 taactgcggc gcgccggtgg cgcggcgggt gccgcccggg gactccctgc cgcgctacgt
    11161 gtgcgacgcg tgcggcgaga tccattacca gaacccgaag ctggtggtcg gcgccctcgc
    11221 cgagcacgag gggcgggtgc tgctgtgccg ccgcgccatc gagccgcgct acggctactg
    11281 gaccctgccc gcgggcttca tggagaacga cgagaccgcg gcccaggccg cgctgcggga
    11341 gacgctggag gaggccggtg cgcgcatcga gctcgccgcg cctttcagca tgatctcggt
    11401 gccgcgcgtc aatcaggtgc atctgttcta ccgggcgcgc gtactggacc tcgagttcaa
    11461 gcccggggag gagagcctgg aagtggccct ggtggacgaa gcgaaggtgc catggaagga
    11521 gcttgccttc cgcaccgtgg cgatcacgct caggcactgg ttcgccgacc gcgcgcgggg
    11581 aaccttcggc ttccacgccg aggacctgcc gctccgctag aatccgcagc aaaacgggga
    11641 ggtcccatga gaaaagcgct gctgttcccg ctgctcgcct tcgcgccgct cgccgcggcc
    11701 gcggaggcaa agctcgatcc cgccaacacc gcctggatga tcacggcatc ggtgctggtg
    11761 ctgttcatga cgctgcccgg cctggcgctg ttctatgccg gcctggtgcg cgccaagaac
    11821 gtcctttcgg tgctcatgca gtgcttcgcc atcgcctgca gtgccacgct ggtctgggtg
    11881 gccgtcggct acagcctcgc cttcagcgac ggcggcgccg ccaacgcctg gatcggcggg
    11941 ctggacaagg cgttcttgct cggcgtggac tcgaagtcgc tctcgggctc gatccccgag
    12001 acggtgttcg cgatgtttca actcacgttc gccatcatca cgccggcgct ggtggtcggc
    12061 gcctacgccg agcgcgtgcg cttcggcggc atgctgctgt tcagcatgct gtggctgctc
    12121 gtggtgtacg tgccggtcgc gcactgggtc tggggcgggg gctggctgca gcagctgggc
    12181 gtgatggatt tcgccggcgg actggtggtg cacctgaatg ccggcctcgc ggcgctggtg
    12241 tgcgcgctgg tcctcggcgt gcgccggggc ttccccgaga ccgccatgcc gccgcacaac
    12301 atgaccatgg cggtcaccgg cgcgtgcttg ctgtgggtgg gctggttcgg cttcaacgcc
    12361 ggcagcgcgc ttgccgccga cggcacggcg ggcatggcga tgctggtgac gcacgtcggc
    12421 gcggcgaccg gctcgctcgc ctggctggtt tgcgaatggc tgcgctacgg caagccttcc
    12481 gtcctgggca tcgtcaccgg catggtggcg ggcctgggaa ccatcacgcc ggcttccggc
    12541 ttcgtcgggc cgctcggcgc catcgccatc ggcgcggccg ccggcgtggt gtgcttcgcc
    12601 gccaccaatt acatgaagcg ctcgctcggc gtcgacgatt cgctcgacgt gttcccggtg
    12661 cacggcgtgg gcgggatcct cgggacgatc ctgaccggcg tgttcgtcgc cgccgccctg
    12721 ggcggcgtcg gcctgccgga cgggcgctcg atgggcggcc aggtcctgat ccagctcggc
    12781 ggcgtgctcg ccatcgcggc ctggtgcggc gtggccacct ggatcctgct caaagtcacg
    12841 caggcggcgg ccggcttgcg cgtgggcagc gagggcgaaa ccgagggcct cgatacccac
    12901 ctgcacaacg aaaaaggcta caacctgtag cggtagaatc gcgcgcatgg agcgccgcga
    12961 tttcatcaag ttctgcgccg cctctgccgc cgcggcgggc gcgcccgccg tttccgcgga
    13021 cgcgcgcccg cacttctacg agcgcgtcaa gctggtgggc gaggaccgcg cgccgctgcg
    13081 cgcgcgcgac gtgccggcga accgcaacct gatcttccac tacccgtacg cgacgacgcc
    13141 ctgctttctg ctcaatctgg gccggccggt gcagggcgtg gcgctgctga agacggagaa
    13201 caaccagccc tatgaatggc gtgggggcgt cggcgcgggg cgctcgatcg tcgcctactc
    13261 ggcgatctgc gcgcacaagc tcacctaccc gacgcgcgac atcagcttca tcagctttcg
    13321 cgccgagaag accaccggca acaagttcgc caacgtgatc cactgctgct ccgagcacag
    13381 ccagtacgac ccggccgcgg gcggccgggt gctggcggga ccggcgccgc aacccctggc
    13441 cgcgatcctg ctcgagcacg acgccgccac cgacgatctc tacgccgtgg gcacgctcgg
    13501 cggcgagatg ttcgagcagt tcttcgccaa gtacgagttc cggctggctt tggagaacgg
    13561 cggcgcgctg cgccgcacgg tgaacggcgt ctcgccggtc acgcccctgg agcgctactg
    13621 caagcagcag gtgaagtgct gaaatggctc ggccccggcg accccttccc gccggtgcaa
    13681 agcgcgctgc aggaccccgc cggactgctg tgcgcgggcg gcgacctgtc ggccgagcgc
    13741 ctgctcgagg gctaccgccg cggcatcttt ccctggtact cgggcgacga gccgatcctg
    13801 tggtggtcgc ccgacccgcg catggtgctg tactgcgacg agctcagggt gagccggtcg
    13861 ctctcgaaga gcctgcgcaa caagggctac gaggtgcgca tcgacaccgc cttccggcaa
    13921 gtgctcgcgg gctgcgccgg cccacgcgcc gacagcggcg gcacctggct cggcgaggac
    13981 atgcgcatcg cgtatgcgcg gctgcaccgt gccggccacg cgcactcgtt cgagacctgg
    14041 cgcggcggcg agctggtggg gggcctgtac ggcgtggcgc tcgggcgcat gttcttcggc
    14101 gagtcgatgt tctcgcgtgc taccgacgcc tccaaggtcg cgctcgtcgg gctggtcggg
    14161 gagctcggcc ggcgcggctt cccgctcatc gactgccagg tcaggacgcc gcttctggcc
    14221 gcgctcggcg cgcgcgagat cccgcggcgc tcgtttttgc gcgaattggc ggcgttggta
    14281 cactacgacg agcctccagg gaaatggacg cgccgaacgg agcccgatgt cccggctgaa
    14341 tgacctgccg cacgcggtac tgcagttcta cgtgaccgcg ccgtacccgt gcagctacct
    14401 gccggaccgc atggcgcgct cgcaggtggc gacgccttcg cacctcatca cgaccgacct
    14461 ctacggcgac ctcgtgaagt cgggctttcg ccgcagcggc atcttcacct accgcccgca
    14521 ctgcgacagc tgccgcgcct gcgtgccggt gcgcatcccg gcctccgagt tcgtcgccag
    14581 ccgctcgcag cgccgcgcct ggagggagca cgccggcctc accgcgcaag tgcagccgct
    14641 gaaattccgc ctcgagcact acgcgctcta tctgcgctac cagtccaagc gccactccgg
    14701 cggcggcatg gacaacgaca gccgcgacca gtactcgcac ttcctgctgc agagccgcgt
    14761 cgacacgcgg ctggtggagt ttcgcgacca gggccagctg gtgatggtgt ccatcgtcga
    14821 ctacctgccc gacggcctgt cctcggtcta caccttcttc gagcccgagc gtcgcggcgc
    14881 gagctacggc acctacaacg tcatgtggca gatcgacgcc tgccgcgcgc tggggatgcc
    14941 gtatctgtac ctcggctact ggatccgcga gagcccgaag atggcctaca agtcgcgctt
    15001 cctgccgatc gagggcttca gcggcggcgg ctggcggcgg ctgtccgagt cggaccttgc
    15061 gcgcatgcgc ttctagcgga tgctctatgc gctggtgcgg cccctgctgt ttcgcctcga
    15121 cccggagctt gcccacgagc tgaccctcgg ccttgccgac gcgccgctgc gcttcggcct
    15181 gatgcgcgtg gcgcgcgccc ccggcgcgcc ggtgcgggcg atggggctgg agttccccaa
    15241 cgcggtgggc ctggccgcgg gcctggacaa gaacgccgag tacgtggacg cgctctcgcg
    15301 cctgggcttc ggcttcctcg aggtcggcac ggtgacgccg cgcccgcagc cgggcaaccc
    15361 gcgcccgcgg ctgttccggc tgccgcgcgc cgaggcgctc atcaaccgca tgggcttcaa
    15421 taacgtcggg ctggacgcat tcctcgacaa cctgtcgcgc gtgcgctggc gcggcatact
    15481 cggcatcaat atcggcaaga atgccgacac gccgatcgag cgcgcggcgg acgactacgc
    15541 ggcctgcttc gagcgcgtgt acgcgcgcgc aagctacgtc accgtcaacg tgtcgtcccc
    15601 caatacgaag aatctgcgcg acctgcagga gaaccgccag ctcgatgccc tgctcgcgcg
    15661 gctcgcgggg ctgcgcgagc cgctcgccca gcgccacggc aagcgcgtgc cgctcgcgct
    15721 caaggtggcg cccgacctgg agggcccgca ggtgcaggcc atcgccgacg cggtgcgccg
    15781 gcacggtgtg gacgccgtca tcgccaccaa tacgtcggtg tcgcgtgaag gcgtggatgg
    15841 gctgcggcat gcgggcgaag caggtggcct gtccggcgcg ccgatccgcg ggcgcgcgac
    15901 ggcgacgctg gccgccctcg ccgccgcgct gcagggcgaa gctgcactcg tcggcgtcgg
    15961 cggcatcctg cgcggcgagc acgccgtgga gaagctcgat gccggcgcgt ctctggtgca
    16021 gctgtacacc ggcctgatct accgcgggcc cgacctggtc gccgaatgcg tatcggcctg
    16081 ccgaaagaaa tcaaggacgg cgagcagcgc gtcgcgctga cgcccgcggg cgccgcagcg
    16141 ctcgagcgcg ccggccacga ggtcgtgttc gagccgggcg cgggtgcggg cgtgggcttt
    16201 ggcgacgagg cctacgcggg cgcgggcgct gcgccgggcg acgcctgggc gtgcgagctg
    16261 gtggtgaaag tcaaggagct gcagcccgcc gagtacgcca ggccgcgccg cgggcagacg
    16321 gtgttcggct tccagcattt cgggcccgag cccgcgctgc tcgaggccgc gctcgcgtcg
    16381 ggcgcgagct tcgtcgcctt cgagaccgtc ggccaggcga gcggcctgcc gatcctcgcg
    16441 ccgatgtcgg ccatcgccgg gcgcctggcg gtgcaggtcg gcgcctggtg cctgcagaaa
    16501 caaaacggcg gcagcggcgt gctgctgccg gggctggacg gcatcgcgcc cggaaaggtc
    16561 gtgatcctcg gcgcgggcaa tgtcggtgcc aatgcgctcg ccgtggcctg cggcatcggt
    16621 gcgtcggtaa gcgtgttcgc gcggagcgag aaacgcttcg cggccctgcg gcagcgctac
    16681 ccggatgcgc ggctctcgac ctcggcgccg gacatcgcgg acgcggacct cgtcatcgcc
    16741 ggcgtgctca cgccggggca gatgtcgccg aagctcatct cgcgcaccat gctgcgcgcc
    16801 atgcgcccgg gctcggcgct cgtcgacgtc ggcatcgacc agggcggcgt cgccgagacc
    16861 tcgcgcccga ccaagcacag cgcgccgatc tacctcgagg aaggcatcgt gcactactgc
    16921 gtcccgaaca tgccggccgc gtgcgcgcgc accgcgagcc tcgcgctgga gcgcgcgctg
    16981 ctgccctacg cgctcaagct cgcggcgggc gagaccgacg ccgatctgga ca
//
LOCUS       DBNHKE010000137        30464 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_181720, whole genome shotgun sequence.
ACCESSION   DBNHKE010000137 DBNHKE010000000
VERSION     DBNHKE010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 30464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_181720"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..198)
                     /locus_tag="AC16O9_09065"
     CDS             complement(4..198)
                     /locus_tag="AC16O9_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516806.1"
                     /translation="MKAKVSTFSATARDFAMLEAVARYHGLNKSATLASLVRKEFWRV
                     FPGGTDKVRPDRGAKVETER"
     gene            483..1724
                     /locus_tag="AC16O9_09070"
     CDS             483..1724
                     /locus_tag="AC16O9_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6516807.1"
                     /translation="MQSFTKHFARIACASTLALALAAPAAAKVEGDKIILGSAISITG
                     KYSQEGKNAMDGYNLSVKKINERGGVKVNGKAYELEVKYYDDESTPARTAQLLERLIN
                     QDGVKYVLGPYSSGTTKAAAPVTEKYKVPMVEAEGASRSLFTQGYKYMFAVLSTSEQY
                     LASAISLAAEVAEQQGKKPSDVRVAMAFENDPFSLDVRAGVVDDIKKYGMKIVVDDKL
                     PRDLSDMSATLTKVKALKPDLLVVSGHTKGASTAGRQINEMGIDVPMIAMTHCESADV
                     VGKFGKGAEGFLCPTQWAETLSYKDPLFGSAMDYNKLFLKTYSGYDTVPYQAAQASAA
                     VQVWADAFERAQSFDTEKLRDAIADTKLDTFYGTIKFSEAGNNVAKPMVLRQIQNGAY
                     NVVAPSKWASHKLAYPRKAGN"
     gene            1787..2689
                     /locus_tag="AC16O9_09075"
     CDS             1787..2689
                     /locus_tag="AC16O9_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516808.1"
                     /translation="MWDNVQILTLAPVFNIQLVLNGVLIGAVFALAAYGLALVWGVTN
                     VKNLAQGDFVMLGGYLTYTLSEYGLHPILALVPVSVLMFAYGWIIYKLVISRVIQRDL
                     FTSLLATFGLAIVMQQSMNLIFGPEVRTADAGFETREMFGGLVTVTDVRMISFALCAL
                     LAIAMVLFMKKSRMGQAIRATAQDARAARVMGIDTERVYAFTFSMNSAICGAAGVLIS
                     MIWVLQPFYGIAYSVRAFVIVTAAGLGNLPGVILAALGLGALEQYSGFVLGAQFQQAT
                     VVGLLVLILVWKQMLLMRHRQSLQ"
     gene            2686..3720
                     /locus_tag="AC16O9_09080"
     CDS             2686..3720
                     /locus_tag="AC16O9_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516809.1"
                     /translation="MTPRAIVLHVAILVAGVAAPLLFPAYTFQFAVLWIMILFALTWD
                     IMGGQMGYNTLGNILFFGLGMYMSAIAQIGMFYDVAEYTAAFGAIKVDFTDAQYFIGL
                     GLGLAFAAAVSVVVAAVLSWVLFGLRGPYFAIGTLGCALAAAELTGAWGYVGGGGGIS
                     MPVFPGEPGVRSLFFYALCMLLTIVCYLFLRWLYGTRFGLALNAVRDDEDKAEAMGVH
                     TSRYKTVAWSISAFFLGMVGALFGNMTGFIEPMEVAYPTLTFGIFMVAMTLLGGKGTL
                     WGPILGATLFHTLKEVTWTYLLGWQWVALGLLIIVNVVYFQQGIMGWLRERKPEWFGV
                     RVESAPGKRA"
     gene            3717..4463
                     /locus_tag="AC16O9_09085"
     CDS             3717..4463
                     /locus_tag="AC16O9_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516810.1"
                     /translation="MSKVLIEVDGVSKAFGGVVANHNVSLRVEEGSITGLIGPNGSGK
                     TTLFNSIAGTHPVDSGSIRFAGREITRLKVQGIARLGLLRTFQQTRIYSKMNCMQNML
                     ISVPHRASTWRDMFRKYPPEAYDRAEHLLEFVGLYQKRLLTAGTLSFGQQKLLEFAMA
                     LMNEPKALLLDEPTAGINPTLINGLIDRLKRANKELGITLFVIEHNMRVIMNLAQRMY
                     CLAHGELLAEGTPEELQNDRRVIDAYLGAH"
     gene            4465..5337
                     /locus_tag="AC16O9_09090"
     CDS             4465..5337
                     /locus_tag="AC16O9_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516811.1"
                     /translation="MSARSKERAGGYHLGDVDTVISVEDVQREAEKLRVAVSADELHG
                     LARGKPFLSIEGLRAGYGNMEILHDFTLHVAKGQSLCLIGPNGAGKSTVLHSIFGFTR
                     IFDGAIFVGEGAARRNVTHLRADRTLKEAGIAYILQDKSVFPDMTVEENLWMGGYLLG
                     SHDAAKTAAEKVFAKYDRLAARRSHKARVLSGGERRLLEISRALVMQPEVLLVDEPSI
                     GLEPRFIDMVFEILRGLQHDDGKTIILVEQNAKKGLEFADLGYVLVSGELALAGSGDE
                     LLENPEVGKLFLGG"
     gene            complement(5783..9124)
                     /locus_tag="AC16O9_09095"
     CDS             complement(5783..9124)
                     /locus_tag="AC16O9_09095"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6516812.1"
                     /translation="MLHLRFDLAFEDLYSRDGLMRLDAAFLQFVAEMDAELCKRLEQA
                     RAAPPPAREESELLIALAPHVEDFVARLFGVETELQALAARHHELAPLYSVKRLFVQR
                     RALHKVKPEDARPDGYRFTTELEFARQITAWQKDEAAHAAELEGAARYAAWAAGTQEG
                     KAKHKAGVLFKAPHKLDLMRLVPAQPEFRHRQGFALTDSGTDLAGALDQANYCIWCHE
                     QGKDSCAKGLKEKAPAGGFKKTVFGVELAGCPLEEKISEFHVVKARGQPIGALAMIAV
                     DNPLAAATGHRICNDCMKACIYQKQDPVDIPQAETRTLKDVLELPWGFEIYALLTRWN
                     PLDLRRPYPRAPSGRRVLVVGLGPAGFSIAHHLMNDGHTVVGIDGLKIEPLEAALSGV
                     DALGRRVPFGPVRDLKELYEPLDERVMAGFGGVAEYGITVRWDKNFLKIIRLLLERRA
                     QFAMFGGVRFGGTLTIDEAFADGFDHIALCAGAGRPTVLETIPNGFARGVRAASDFLM
                     ALQLTGAAKRESIANLQIRLPIVVIGGGLTAIDTATESLAYYAVQVEKFLSRWEALEE
                     KPQWTAEEQEIAQEFIAHARALRAAGPEQRLPLLRSWGGSTIAYRRRMVDSPSYTLNH
                     EEVAKALEEGIVFREGLTPQRIEVDAHGHARAVVFQDGLTLPARSVLIAAGTQPNTVL
                     AREEGVSLALDGKYFMACDERGAPVKPERHTAKPARPDVLLHRHASGRFMSFFGDLHP
                     SYFGNVVKAMGSVKQGYPVVSRVLAQNPPAIAIDDAAFLSRLNRDLRAVVHEVRRHTP
                     TIVEVVLRAPIAARRFRPGQFYRMQNYETLALRVPGTTLAMEGLALTGAWTDRDKGLI
                     SVIALEMGGSADLLAYLQPGEPVVLMGPTGTPTEIPRGETVVLAGGGLGNAVLFSIGR
                     ALRAAGSRVIYFAGYRHLGDRYRIEDIESASDVVVWCCDEAPGFEPRRTKDRAFVGNI
                     VQAMHAYASGALGAQPIAFSATDRIIAIGSDRMMAAVARARRDVLAPHLKPGHVAIGS
                     INSPMQCMMKEICAQCLQPHVDAATGKTTYVFSCFNQDQPLDEVDFGALGQRLAQNSV
                     QEKLTARWIARCLKALPPPAERKAA"
     gene            9174..9935
                     /locus_tag="AC16O9_09100"
     CDS             9174..9935
                     /locus_tag="AC16O9_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR6516813.1"
                     /translation="MLIKNSVFLVTGGASGLGAATARMAAENGAKVVIADMQVEAGEK
                     LAQELGGRFVKCDVTAEADAKAAVALALKEFGGLQVLVNCAGIALAERTIGKEAPHDL
                     ARFTRVITVNLIGTFNMIRVAADAMAKAGPNAAGERGVIINTASVAAYDGQIGQAAYS
                     ASKGGVVGMTLPIARDLSRNGIRVCTIAPGIFETPMLLGLPKEAQDSLGKQVPFPSRL
                     GRPAEYAQLARAVIENEMLNGETIRLDGAIRMAPK"
     gene            complement(9949..11526)
                     /locus_tag="AC16O9_09105"
     CDS             complement(9949..11526)
                     /locus_tag="AC16O9_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6516814.1"
                     /translation="MSGTLQRGTLHLGQVVATHARLQPDALGARDSRRSITFAQWNAR
                     ACRLANALAGLGLRKGDRVAVLAFNRLEWMEIYVGLAKAGLTAVPVNFRLLAPEIRYI
                     VAHCGARAFIAEDSLIDRVEPVRGELPAVRGGWIRLGDGEAAGWTGYEALLAAASDRE
                     PQADVRPLDPWALMYTSGTTGRPKGAIRNHQGSALISLVTALDMGLTRADKALLVMPM
                     CHANSLYFAFTFTYLGAPCIIDDRASFDPEALLATIAAEGVTFTSLVPTHYIMTLALP
                     AATKARYDVGRVRKLMISSAPARKDTKLAILEYFRNSQLYELYGSTEAGWVTLLRPHE
                     QLEHLGSVGREWTGTGPVRILDAEGRDLPDGEVGELFSLTPYAFDGYLDDPAKTAEAL
                     RPDGYCSVGDMARRDGKGFVYLVDRKSNMIISGGENVYPSEIEDILGRHPAVKDVAVI
                     GVPHEKWGEAVHAVVVLREEVAATEAAILDWCRDKMAGFKRPRSVSFLREADMPRTAT
                     GKIQHRMLRERLSRSQV"
     gene            complement(11523..13232)
                     /locus_tag="AC16O9_09110"
     CDS             complement(11523..13232)
                     /locus_tag="AC16O9_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6516815.1"
                     /translation="MSTARTVAEWIVEFLVAQGVDRVYGLQGGHIQPIWDWLAQRGVR
                     IIDVRDEGAAVHMAHCHAVLTGQVGVALATAGPGVTNCVTAVANAQLERVPVLLIGGC
                     APVQQDDLGPLQGIDHVAILRPVTRSARTLRAPDNILRDLDKGWSTALGDGNPPGPVY
                     LEIPTNVLRLKIPPRLALPEYLSRRRVRHVPPDSADVGRAAALLRAARRPLVVTGRGA
                     LGTAPPLRALLEACGAVYLDTQESRGLVPGSHPSFVGAVRARAMQEADLVVTVGRKLD
                     YQMAYGSPAVFANARWIRIADNAEELRENRRGEVELFANPGLALSAIAAALTSAGALD
                     KAWTRGLREEHERRSSKYAASLATAPAGKDGHMHPNRIFAALRKLLAPDAITIADGGD
                     ILSFARLGLEAPSTYLDPGTFGCLGVGIPYGNAAALLHPGRQVVVVSGDGAFGINAME
                     VDTAKRHGAGAVFIIANNAAWNIERLDQELNYGGRVVGTTLAWSDYAAMARAFGLHAE
                     RVTDADRLEGAIAEALAKAPALIDVVVTQHALSSDAGKGLGWVPDYQALTAWDEAEKA
                     RRA"
     gene            complement(13255..13767)
                     /locus_tag="AC16O9_09115"
     CDS             complement(13255..13767)
                     /locus_tag="AC16O9_09115"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR6516816.1"
                     /translation="MKLAGAGHFIAYDATLVGDITLQSDVNIWFKVVIRAENDAITIG
                     ERTNVQEGAVLHVDPGFPLAIGANVTVGHRAVLHGCLIGDGALIGINAVVLNGAKIGA
                     GALIGAGALVPEGKEIPPGVLVVGSPGRVQRELRPAEREMLRHASQVYVERSKFYAAA
                     MREQPMPAPQ"
     gene            13815..15062
                     /locus_tag="AC16O9_09120"
     CDS             13815..15062
                     /locus_tag="AC16O9_09120"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HIR6516817.1"
                     /translation="MRIVVLGAGVIGVTTAWYLARDGHKVTLVDRQAEAGMETSFANG
                     GQVTAGHAEPWANPSALPKVLRWLGREDAPLLFRPRADRAQWLWALRFALECLPGRFA
                     RNTRQLAGLARYSRDCLAELRAQTGIEYERVARGILHFCTEHADFEALAKHAEDMRRL
                     GIEREVKSAAQCHDLEPALRASQARVVGGVYTPQDESGDAHEFTVRLAQLAEQSGVRF
                     LRRATVESLDVEGGAVSAVRAGGERLQADAVVVSLGSYSARLLAPLGISIPVYPLKGY
                     SVTLTLPPEVATQAPTISLTDEGRKLVISRFGARLRCAGTAELTGYDTRINAKRCEAI
                     VARLRVLFPALARAGAAELWAGLRPATPSNVPVIGRTRLANLYLNTGHGTLGWTLAAG
                     SGRALADLLAGRRPDVDFAFRGA"
     gene            15059..15931
                     /locus_tag="AC16O9_09125"
     CDS             15059..15931
                     /locus_tag="AC16O9_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6516818.1"
                     /translation="MKASASLYYDVRGLRYHVRHWPGEGGPRVLLLHGWMDVSASFQF
                     LVDALPAHWEVFAPDWRGYGLTDWGKSDCYWFPDYLADLDALLQQLSPQAPVDLVGHS
                     LGGNVACLYAGVRPSRIRRLANLEGFGMAVTRPDQAPARYARWLDELGSSKGFRPYAN
                     FAALAERMRQSNPRLAPERAEFLSRHWGRELPDGQVQLRGDPAHKIVYPVLYRYEEAR
                     ACWSEVTAPVLWVDAAESKSLGRVGLDAARYAERRAAFRNLRYVTVSNAGHMLHHDQP
                     EQLARLLEEFFGAP"
     gene            15928..16758
                     /locus_tag="AC16O9_09130"
     CDS             15928..16758
                     /locus_tag="AC16O9_09130"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HIR6516819.1"
                     /translation="MNIDLHCHSNVSDGALAPGEVARRAARAGVAVWALTDHDQLAGL
                     AEARAAARDEGMRFVDGVEISVTWRGATVHVVGLRIDPGNADLAAGLAAVRGGRLQRA
                     RAMAHDLQRAGIDGTYEGAIERAENPAMVSRTHFARFLVDSGVVADMREAFRRFLVPG
                     KPGYVPHQWAELADAVRWIGAAGGQAVLAHPGRYGLSAGAMDALLQEFRAAGGEAIEV
                     VTGSHSAEQSRHFAALAAQYDFSASRGSDFHGPDEGAEFGTLPPLDPALRPVWREWGL
                     "
     gene            16768..17397
                     /locus_tag="AC16O9_09135"
     CDS             16768..17397
                     /locus_tag="AC16O9_09135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6516820.1"
                     /translation="MAQYFAVHPTHPQPRLIRQAADIVRGGGLIAYPTDSCYALGCHL
                     GDADAQARLRRVRGMDDKHHLTLMCRDLSEIATYAIFDNAQFRLLKAATPGSYTFVLR
                     ATREVPRRLMHPRRKHIGVRVPAHPAARALLEEMGEPMLSATLLLPGSAVALSDAQQI
                     RAALEHQLDLVIDSGPCGTEPSTVIDLTGEAPVVLRAGRGSLAVLGQSV"
     gene            17417..18082
                     /locus_tag="AC16O9_09140"
     CDS             17417..18082
                     /locus_tag="AC16O9_09140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6516821.1"
                     /translation="MDVTQLVQTLAIYSLPVLFAITLHEAAHGYVARYFGDVTAYAQG
                     RISLNPFRHIDLVGTIIVPIAILVAAKLVGGAGLLFGWAKPVPVNYSALRRPKQNMLW
                     VAAAGPGANLAMALAWALLLKLTEALALSQFSVVLRLVCVAGIQVNVVLMVLNLLPIL
                     PLDGGRILASLLPHRMAWQYARLEPWGFPILLLLLFSGYLGVVLRPVVEASYLVIGSV
                     LQL"
     gene            18085..19287
                     /locus_tag="AC16O9_09145"
     CDS             18085..19287
                     /locus_tag="AC16O9_09145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6516822.1"
                     /translation="MYETRVLSGMRPTGALHLGHYHGVLKNWVRLQAEYPCLFFVADW
                     HALTTDYEHPGRVEQNAWEMAVDWLAAGVDPNQAILFVQSHIQEHAELHLLLSMMTPL
                     GWLERVPTYKDQMEKLKDRDLATYGFLGYPLLQSADILIYRARYVPVGEDQVPHVEMT
                     REIARRFNHLYGREEGFEDKAKSAVKKMGTKKARLLNELRTKFLESGDADALKRAQAL
                     LEEQQNLPRGDRERLYGWLEGGGRKILVEPEALLTEAARLPGLDGQKMSKSYGNAIGL
                     RESPESVTKKIRTMPTDPARVTRKDPGTPEKCPVWPLHLVYSDTARRDWVQKGCTTAG
                     IGCLECKQPVIEAVLAELAPMRERAQVYVDDPTLVKSILADGCDKARKLAVETMRDVR
                     ESMGLTYT"
     gene            19284..20090
                     /locus_tag="AC16O9_09150"
     CDS             19284..20090
                     /locus_tag="AC16O9_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6516823.1"
                     /translation="MIEPAPLALATPVARLYGEPLIEIPQDLYIPPDALEVILEAFEG
                     PLDLLLYLIRKENLDILDIPMAPLTRQYLEYVEAMRATNLELAAEYLVMAATLMAIKS
                     RLLLPRPAAAEPEEEDPRAELVRRLLEYERIKKAAQALDELPQVGRDVLAVSVWVERT
                     VAERLPEVNPQDLAEAWRALLHRARLSAHHHVSREELSVREHMSGILRRLRERRVLEF
                     GELFDPARGVAVLVVTLLAVLELAREMLLELTQSESFAPIYVKLAHAEPL"
     gene            20074..20655
                     /gene="scpB"
                     /locus_tag="AC16O9_09155"
                     /pseudo
     CDS             20074..20655
                     /gene="scpB"
                     /locus_tag="AC16O9_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            20603..21391
                     /gene="rluB"
                     /locus_tag="AC16O9_09160"
     CDS             20603..21391
                     /gene="rluB"
                     /locus_tag="AC16O9_09160"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF007976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HIR6516824.1"
                     /translation="MNPRRRPPSPKPPTNPPQAERLQKLLAAAGLGSRRQVETWIEAG
                     RLSVNGRTARLGERALASDRIELDGKALTLKASGARPRVLLYHKPDGEMVTRADPEGR
                     PTVFQRLPPIEGGKWVAVGRLDINTAGLLLLTDSGELANRLMHPRYEVEREYAVRVLG
                     ALSEENRTRLTRGVQLADGTGRFDSLAPSGGAEGANRWYRVVLREGRNREVRRLFEAV
                     GSKVSRLLRVRYGPVALPRDLRPGRWIELDEKSVAEIVKAAKSG"
     gene            21438..21887
                     /gene="rimP"
                     /locus_tag="AC16O9_09165"
     CDS             21438..21887
                     /gene="rimP"
                     /locus_tag="AC16O9_09165"
                     /inference="COORDINATES: protein motif:HMM:NF000929.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR6516825.1"
                     /translation="MAASVPNSLEGLLEPTLAGLGFELVDAQVSNRGRFVRIFMDKPG
                     GITVDDCAAVSRHLSHLLEVEGIDYDRLEVSSPGMDRPLRRPADFARFLGHRVDVRMR
                     LADATGRRRYVGVLRGIDGATATVEVDGAPVALQLEGMDRARLVPEL"
     gene            21901..23409
                     /gene="nusA"
                     /locus_tag="AC16O9_09170"
     CDS             21901..23409
                     /gene="nusA"
                     /locus_tag="AC16O9_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6516826.1"
                     /translation="MSREILLLVDALAREKNVNRDVVFGALEMALASATKKRFSEDID
                     VRVAIDRNTGDYEAFRRWHVVPDDTPEWNTAQMLSLEEAQDRKADAQMDEYLEQPIEK
                     IPFGRIGAQTAKQVILQRIRDAEREQILNDFLGRGDELVTGSVKRMERGNAIVESGRL
                     EALLPREHMIPKENLRVGDRVRAWVMKIDRGARGPQLVLSRTAPQFIMKLFELEVPEI
                     EEGLLAIKSAARDPGVRAKIAVHTNDRRIDPIGTCVGMRGSRVQAVTQELAGERVDIV
                     LWSADPAQFVIGALAPAEVSSILVDEEKHAMDVVVDEENLAIAIGRSGQNVRLASELT
                     GWTINLMTEEESTKKQAEESTRVRELFMQRLDVDEEVADILIQEGFSTLEEVAYVPIN
                     EMMEIESFDENTVNELRSRARNALLVQAIASEEKVEGVDQNLLQLEGMDTQLAAKLAE
                     GGVKTRDDLADLAVDELSEMTGIEEERAKGLIMAARAHWFEQDGAAQLKEAQ"
     gene            23411..25999
                     /gene="infB"
                     /locus_tag="AC16O9_09175"
     CDS             23411..25999
                     /gene="infB"
                     /locus_tag="AC16O9_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR6516827.1"
                     /translation="MAMSSVAQFASELKMPPAVLLEQLRAAGVEKRAPDDSLTESDKA
                     RLLEYLRKSHGSVEPKNKITLTRKQTSEIIKKSDTSGRARTIQVEVRKKRVFVKRDPA
                     ELAAQEGAAEAPAPAPAAPAPAPAPAPIPAATPAIDERERQAREDEARRHEQLSALQA
                     AELKEKQERERREAEQRAAEREAQVAIERAQAQPGAAPPVEGTLHAPKTDAKPDKKPK
                     KAKPTTTMRDDSVRRRTIKTRGDMGGGVAGWHARGGARHRGAAGAEQQTFSQPAEPVT
                     REIAVPETIAVGELAHRMSVKAAEVIKVLMKLGTMATINQALDQETAMIVVEEMGHKA
                     KAAKLDDPEAYLAESAQDAAQEGVAGEPRPPVVTVMGHVDHGKTSLLDYIRRTKVAAG
                     EAGGITQHIGAYHVETPRGVITFLDTPGHEAFTAMRARGAMVTDLVILVVAADDGVMP
                     QTVEAINHAKAAEVPLVVAINKIDKGEANPEKVKQELLQHGVVPEEFGGEVAFVPVSA
                     KTGKGVDDLLERVLLQAEVLELKAPAQTPARGVVIEARLDKGRGPVATVLVRSGTLHR
                     GDVVLTGAVYGRVRAMLDENGKPIESAGPSIPVEIQGLSEVPTAGEDMATLADERKAR
                     EIALFRQGKFRDVKLAKQQAAKLENLFQQMGEGDKKVLSLVIKADVQGSQEALVHALT
                     RLSSEEVKVGVVHAGVGGITESDVNLALAAKAVIIGFNTRADATARKLIESSGIEVRY
                     YNIIYEAVDEIKAALSGMLAPERKEQVLGLVEVRQVFRISKVGAVAGCYVVDGLVRRN
                     AQVRVLRDNVVIHTGEIDSLKRFKDDTREVKAGFECGMSLKNFSDIKEGDQFEVFEVL
                     EVARTL"
     gene            25996..26340
                     /gene="rbfA"
                     /locus_tag="AC16O9_09180"
     CDS             25996..26340
                     /gene="rbfA"
                     /locus_tag="AC16O9_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR6516828.1"
                     /translation="MSRGRPQKVADQIQRELSLLLSRELRDPRLGLVTLTAVDVAPDL
                     SHAVVYFTCLDAAQVEPATTALQHAAGYLRTHLAKRIKLYTTPELRFRHDESVARGAY
                     LSQLIDSVRPKR"
     gene            26337..27230
                     /gene="truB"
                     /locus_tag="AC16O9_09185"
     CDS             26337..27230
                     /gene="truB"
                     /locus_tag="AC16O9_09185"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR6516829.1"
                     /translation="MMRRRLDGVLLLDKPPGVGSNAVLQTVKRLYGAEKAGHAGTLDP
                     LASGLLPVLFGEATKFSQLALDADKAYIATARLGITTTTGDAEGEVLERKPCNVRAED
                     VERALAAFRGELEQVPPMYSALKHGGQPLYALARAGREVPRAARRIRVHALELLRFGG
                     DSLELRVRCSKGTYVRTLVEDIGRALGCGAHVSALRRTASGMFRIEDAVTLEQLQAEI
                     PGARDARLLPLERLLGGLPRVDLPAALAERFLRGQALQVEHAPSGPCGVYGQQGRLLG
                     LGEGRAQGELRPRRLLSYASG"
     gene            27305..27574
                     /gene="rpsO"
                     /locus_tag="AC16O9_09190"
     CDS             27305..27574
                     /gene="rpsO"
                     /locus_tag="AC16O9_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR6516830.1"
                     /translation="MALQVAQKAQVVQQYQRSNKDTGSPEVQVALLTERINSLAEHFK
                     TNVKDFHSRRGLLKLVSQRRKLLDYLRRTDADKYRSLIERLSLRK"
     gene            27649..29763
                     /gene="pnp"
                     /locus_tag="AC16O9_09195"
     CDS             27649..29763
                     /gene="pnp"
                     /locus_tag="AC16O9_09195"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6516831.1"
                     /translation="MNPTKKTFTLGDQQVTFETGEIARQAHGAVLCTIDDTVVLATVV
                     GAKEPKAGQDWFPLTVDYAERTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PDGFFNEVQVVAQVLSLNPEVDSDIPAMLAVSAALTLSGIPWNGPIGACRVGYVNGQY
                     VLNPSATQLKESKLNLVVAGTEQGVLMVESEAHELAEDVMLGAVEFGHAKMQAAIQAI
                     DELAEVAAKPAWDWQPPAKNEALIQRIAELAEADLRAAFEMRQKQARNTRLNEIRKAT
                     LAKVDEEAKAAGTELAPNVAKDVFFNLESKIVRNQILDGEPRIDGRDTRTVRPINVRV
                     GVLPRTHGSALFTRGETQALVSVTLGTGRDEQIIDALAGEYRERFMFHYNMPPYATGE
                     TGRFGFTKRREIGHGRLAKRALVAMLPGAEDFGYSLRVVSEITESNGSSSMASVCGGS
                     LALMDAGVPITAHVAGIAMGLIKEGNRFAVLSDILGDEDHLGDMDFKVAGTETGVTAL
                     QMDLKIESINKSIMKIALDQAREARLQILTIMKQAIEGSRTELSSFAPRIIKIKINPD
                     KIRDVIGKGGVVIRGLQEETGTTIEIEDDGTISIACVSAEGGEAAKKRILDITADVEV
                     GKIYEGPVLRLLDFGAIVQLLPGRDGLLHISQISHERVNAVSDHLKEGQIVKVKVLEA
                     DDKGRVRLSMKAIGAPEAAKQA"
     gene            complement(29812..30237)
                     /gene="dksA"
                     /locus_tag="AC16O9_09200"
     CDS             complement(29812..30237)
                     /gene="dksA"
                     /locus_tag="AC16O9_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR6516832.1"
                     /translation="MAVELTRKSFTPYAPKKGEDYMNARMRTHFRKMLEQLKAELSEE
                     IDRTVHTMQDEATVFADPNDRASQESDMALELRNRDRERKLIKKIDETIGRIDANEYG
                     YCESCGVEIGLKRLEARPTATLCIDCKTLDELREKQVAK"
     gene            30329..30405
                     /locus_tag="AC16O9_09205"
     tRNA            30329..30405
                     /locus_tag="AC16O9_09205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30363..30365,aa:Pro,seq:tgg)
BASE COUNT         5002 a        10010 c        10697 g         4755 t
ORIGIN      
        1 aggtcatcgc tcggtctcca cctttgcacc gcgatcaggg cgcaccttgt cagtgccgcc
       61 cgggaacacg cgccagaatt ccttgcgcac caggctggcg agcgtggcgc tcttgttgag
      121 cccgtgatag cgcgccacgg cctcgagcat ggcgaagtcg cgcgcggtgg ccgaaaacgt
      181 gctgaccttg gctttcatca tatataaaag atatataacc cttaaattaa tgtcaagcgc
      241 tttccagggc cttcggaggg attgttgcaa tgcgccagtc acgcctcgct gttatagcgc
      301 tgcggcccat gctgccctgc cgcatccggg cccgcgcggc aagttgcggt tgccccgcgc
      361 catgaactcg tgcgagattc ggcagtcgta ccgtaggcgc tgcgccgcaa gccaacaagc
      421 cgctcccgca cagaggagtt tcgcgcggca ccgttcgatc aacactcgga gaggaggaga
      481 cgatgcaatc gttcacgaag catttcgcac gcatcgcgtg tgcgtcgacc ttggccctgg
      541 cgcttgccgc accggcagcc gcgaaggtcg aaggcgacaa gattattctc ggctcggcga
      601 tctcgattac cggcaagtac tcgcaggaag gcaagaacgc gatggacggg tacaacctct
      661 cggtgaagaa gatcaacgag cgcggcggcg tgaaggtgaa cggcaaggcc tatgagctcg
      721 aggtcaagta ctacgacgac gagtcgacac cggcgcgcac ggcgcagctc ctggagcgct
      781 tgatcaacca ggacggcgtg aagtacgtgc tcggaccgta cagctcgggc accaccaagg
      841 cggccgcgcc ggtcaccgag aagtacaagg tgccgatggt ggaggccgag ggcgcgtcgc
      901 gctcgctctt cacgcagggt tacaagtaca tgttcgcggt cctgtcgacc tccgagcagt
      961 acctggcgag cgccatctcg ctcgcagccg aggtcgcaga gcagcaaggc aagaagccga
     1021 gcgacgtgcg cgtcgccatg gcgttcgaga acgatccgtt ctcgctcgac gtgcgcgcgg
     1081 gcgtcgtgga cgacatcaag aaatacggca tgaagatcgt ggtggacgac aagctgccgc
     1141 gcgacctgtc cgacatgtcg gccacgctca ccaaggtgaa ggcgctcaag cccgacctgc
     1201 tggtggtctc gggtcatacg aaaggggcca gcacggcggg acgccagatc aacgagatgg
     1261 gcatcgacgt gccgatgatc gcgatgacgc actgcgagtc ggcggacgtg gtcggcaagt
     1321 tcggcaaagg cgcggaaggc ttcctgtgcc cgacgcagtg ggccgagacg ctgagctaca
     1381 aggatccgct gttcggcagc gcgatggact acaacaagct gttcctcaag acctactcgg
     1441 ggtacgacac cgtgccctac caggccgcgc aggcctcggc cgctgtgcag gtctgggcgg
     1501 acgcgttcga gcgcgcgcag agcttcgaca ccgagaagct gcgcgatgcg atcgccgaca
     1561 ccaagctcga cacgttctac ggcacgatca agttcagcga ggccggcaac aacgtggcca
     1621 agccgatggt gctgcgccag atccagaacg gcgcgtacaa cgtcgtggcg ccgtcgaagt
     1681 gggcgtcgca caagctcgcg tacccgcgca aggcggggaa ctagacctgt cggcttgagg
     1741 agcagagaga ctgcgccggc cgcttgcggc cggcgcggca tttgccatgt gggataacgt
     1801 ccagatcctg acgctggcgc ccgtgttcaa catccagctg gtgttgaacg gggtgctgat
     1861 cggcgccgtg ttcgcgctcg ccgcctacgg tttggcgctg gtgtggggcg tcacgaacgt
     1921 caagaacctg gcgcagggcg acttcgtgat gctgggcggc tacctgacct acactctttc
     1981 cgagtacggc ctgcacccga tcctggcgct ggtgccggtg agcgtgctca tgttcgcgta
     2041 cggctggatc atctacaagc tggtcatcag ccgcgtgatc cagcgggacc tgttcacctc
     2101 gctgctcgcg actttcggcc tggccatcgt catgcagcag tcgatgaatc tgatcttcgg
     2161 gccggaggtg aggaccgccg acgccggctt cgagacgcgc gagatgttcg gtggcctggt
     2221 cacggtgacc gacgtccgga tgatctcgtt cgcgctgtgc gcgctgctcg cgatcgccat
     2281 ggtgctgttc atgaagaaga gccgcatggg gcaggcgata cgcgccaccg cgcaggacgc
     2341 gcgcgctgcg cgcgtcatgg gcatcgacac cgagcgggtg tacgccttca ccttctccat
     2401 gaattccgcg atctgcggcg ccgcgggggt gctgatctcg atgatctggg tgctgcagcc
     2461 gttctacggc atcgcttact cggtgcgtgc gttcgtcatc gtaacggcgg ccggcctggg
     2521 caacctgccg ggggtgatcc tggcggcgct cggtctgggc gcgctcgagc agtacagcgg
     2581 cttcgtgctc ggcgcgcaat tccagcaggc gacggtggtg ggactgctcg tgttgatcct
     2641 ggtatggaag cagatgctgc tgatgcggca tcggcagtct ctgcaatgac gccgcgcgcc
     2701 atcgtgctgc acgtcgccat cctggtcgct ggcgtggcag cgccgctcct gttcccggcc
     2761 tacacgttcc agttcgcggt gctctggatc atgatcctgt tcgcgctcac ctgggacatc
     2821 atgggtggcc agatgggcta caacacgctc ggcaacatcc tgtttttcgg gcttggcatg
     2881 tacatgagcg ccatcgcgca gatcggcatg ttctacgacg tcgccgagta cacggcggct
     2941 ttcggcgcca tcaaggtgga cttcaccgac gcgcagtact tcatcggcct ggggctggga
     3001 ctggcgttcg ccgcggcggt gagcgtggtt gtggccgcgg tgctgagctg ggtgctgttc
     3061 ggcctgcgcg ggccatactt cgccatcggc acgctgggct gcgcgctcgc cgccgcggag
     3121 ctgaccggcg cctggggcta cgtaggcggc ggcggcggca tctcgatgcc cgtgttcccg
     3181 ggagagcccg gcgtgcgctc gctgttcttt tatgcgctgt gcatgctgtt gacgatcgtc
     3241 tgctacctgt tcctgcgctg gctgtacggt acgcgcttcg ggctggcgct gaacgccgtc
     3301 cgcgacgacg aggacaaggc cgaggcgatg ggcgtgcaca ccagccgtta caagacggtg
     3361 gcctggtcga tctcggcgtt cttcctcggc atggtgggtg cgctcttcgg caacatgacg
     3421 ggattcatcg agccgatgga ggtggcgtat ccgaccctga ccttcggcat tttcatggtg
     3481 gcgatgacgc tgctgggcgg caagggcacg ctgtggggcc cgatcctcgg cgcgacgctg
     3541 tttcacacgc tgaaggaggt cacctggacc tatctcctcg gctggcagtg ggtggcgctc
     3601 ggcctgctga tcatcgtaaa cgtcgtctac ttccagcagg gcatcatggg ctggctgcgc
     3661 gagcgtaagc ccgagtggtt cggcgtgcgg gtggagtctg cgccgggaaa acgcgcatga
     3721 gcaaagtgct catcgaggtg gacggggtga gcaaggcctt tggcggggtg gtcgccaatc
     3781 acaacgtttc gctgcgcgtc gaggagggca gcattaccgg actcattggc ccgaacggct
     3841 cgggaaagac gacgctgttc aactccatcg ccggcaccca tcccgtcgac agcggctcga
     3901 tccgcttcgc gggccgcgag atcacccgcc tcaaggtgca gggcatcgcg cggctgggcc
     3961 tgctgcgcac gttccagcag acccgcatct actccaagat gaactgcatg cagaacatgc
     4021 tgatctcggt tccgcaccgc gccagcacat ggcgcgacat gttccgcaag tatccgcccg
     4081 aggcctacga ccgcgccgag catcttctcg agttcgtcgg tctgtaccag aagcgcctgc
     4141 tgacggcggg cacgctgtcc ttcggccagc agaaactgct cgagttcgcg atggcgctca
     4201 tgaacgagcc caaggcgctg ctgctcgacg agcccacggc cggcatcaac ccgacgctta
     4261 tcaacggcct catcgaccgg ctgaagcgcg ccaacaagga gctcggcatc acgctgttcg
     4321 tcatcgagca caacatgcgc gtgatcatga acctcgccca gcgcatgtac tgcctggcgc
     4381 acggcgagct gctcgccgag ggcacgcccg aggagctgca gaacgatcgt cgcgtgatcg
     4441 acgcttatct cggggcgcat tgacgtgagc gccaggtcca aggaacgcgc cggcggctac
     4501 cacctcgggg acgtcgacac cgtcatctcg gtcgaggacg tccagcggga agccgagaag
     4561 ctgcgcgtcg ccgtgagcgc ggacgagctg cacgggctgg cgcgcggcaa gccattcctg
     4621 tccatcgaag gactgcgcgc cggctacggc aacatggaga tcctgcacga cttcaccctg
     4681 catgtggcga agggccagtc gctgtgcctg atcggcccga acggggccgg caagtccacc
     4741 gtgctgcact cgatcttcgg cttcacgcgt atcttcgacg gcgcgatctt cgtcggcgaa
     4801 ggcgcggcgc gccgcaacgt cacgcacctg cgcgccgacc gcaccctgaa ggaagccggc
     4861 atcgcctaca tcctgcagga caaatcggtg tttccggaca tgacggtgga ggagaacctg
     4921 tggatgggcg gctacctgct cggctcgcac gacgcggcga agacggccgc cgagaaggtg
     4981 ttcgccaagt acgatcgcct ggccgcccgg cgcagccaca aggcgcgcgt gctctcaggc
     5041 ggcgagcgac ggctgctgga gatctcgcgc gcgctggtga tgcagcccga agtgctgctg
     5101 gtcgacgagc cttcgattgg cctcgagccg cgcttcatcg acatggtgtt cgagatcctg
     5161 cgcggcctgc agcacgacga cggcaagacc atcatcctgg tcgagcagaa cgcgaagaag
     5221 gggctggagt tcgccgacct gggctacgtc ctggtgtccg gcgagctcgc gctagccggc
     5281 agcggcgacg agctgctcga gaatcccgag gtcggcaagc tgttcctcgg cggctagccg
     5341 gagctgacct cgatcgttgc ggcttcgcgt gcgcggctgg gagttgacgc gcacctggtc
     5401 ggaatcgagg tcgtcggaga gggccaccat gcagtgaaag cgcgccgcga agcgcccgtt
     5461 cacgtgaacg ctcgcctccc ggaagccgac cagccgttcg cccgctgagc accccgcgct
     5521 cgagctgcac gcggattgcg gcgggatcga gtccaggggc gacggcgtag atctcggcgg
     5581 actgaggggt accggcgtta agcgcaggga agccgcggat gctgggcgca aattcaaagg
     5641 gttgctgcac ctggcgctgc ggccggtcca gcgccgcgat caggtcgcgg tgaaatagcg
     5701 acgtatccga cgtgtccatg gcgcggtctc cctccagccg gccggggcgg agacaggagc
     5761 gccccgcgcg cgatcaagcg ggtcaggccg ccttgcgctc cgcgggcggc ggcagcgcct
     5821 tcaggcagcg cgcgatccag cgggcggtca gcttctcctg cacgctgttc tgtgccaggc
     5881 gctggccgag cgcgccgaag tccacttcgt cgagcggctg gtcctggttg aagcaggaga
     5941 agacgtaggt cgtcttcccc gtcgccgcgt cgacatgggg ctgcaggcac tgcgcgcaga
     6001 tctccttcat catgcactgc attggagagt tgatcgaacc gatcgccacg tggccgggct
     6061 tcaggtgcgg cgcgaggacg tcgcgtcgcg cgcgcgccac ggccgccatc atccggtcgg
     6121 agccgatggc gatgatgcgg tcggttgccg aaaacgcaat gggttgggcg ccgagggcgc
     6181 cggaagcgta ggcatgcatc gcctggacga tgttgccgac gaaggcgcgg tcctttgtcc
     6241 gtctcggctc gaaccccggc gcctcgtcgc agcaccagac cacgacgtcg gaggcgcttt
     6301 cgatgtcctc gatgcggtag cggtcgccga ggtgcctgta gccggcgaaa tagatcactc
     6361 ttgaaccggc ggcgcgcagc gcgcggccga tggagaacag caccgcgttg ccaagaccgc
     6421 cgccggccag caccacggtc tcgccgcgcg gaatctccgt cggcgtgcca gtgggaccca
     6481 tcaggaccac gggctcgccg ggctgcaggt aggcaaggag gtcggccgag ccgcccatct
     6541 cgagcgcgat caccgagatc agccccttgt cgcgatcggt ccaagcaccg gtgagcgcga
     6601 gaccctccat cgcgagcgtg gtgcccggca cgcgcagcgc cagcgtctcg tagttctgca
     6661 tgcggtagaa ctgccccgga cggaagcgcc gcgccgcgat cggcgcgcgc aacactacct
     6721 ccacgatggt cggcgtatgc cggcgcacct cgtgcaccac ggcgcgcagg tcacggttca
     6781 ggcgcgaaag gaaagccgca tcgtcgatcg cgatggccgg cggattctgt gccagcaccc
     6841 ggctgacaac ggggtagccc tgcttgacgc tgcccatcgc cttcaccacg ttgccgaagt
     6901 aggacgggtg cagatcgccg aagaagctca tgaagcggcc gctggcatgg cgatgcagca
     6961 gcacgtctgg gcgcgcgggc ttggcggtgt ggcgctcggg cttgaccggg gcgccgcgct
     7021 cgtcgcaggc catgaagtac ttgccgtcga gcgcaaggct cacgccttct tcgcgcgcga
     7081 gcacggtgtt gggctgcgtg cccgcggcga tgagcaccga gcgcgcggga agcgtgaggc
     7141 cgtcctggaa cacgaccgcc ctcgcgtggc cgtgcgcgtc gacctcgatg cgctgcgggg
     7201 tcagaccttc cctgaagacg atgccttcct cgagcgcctt cgcgacctcc tcatggttga
     7261 gggtgtagga ggggctgtcg accatgcggc gccggtaggc aatggtcgag ccgccccagg
     7321 acctgagcag cggtagcctt tgttcgggcc cggccgcgcg caaagcgcgc gcgtgggcga
     7381 tgaactcctg cgcgatctcc tgctcctcgg ccgtccactg cggcttttcc tccagcgcct
     7441 cccagcgcga caggaacttc tccacttgca ccgcgtagta ggcgagggac tccgtcgccg
     7501 tgtcgatggc ggtcaggccg ccgccgatca ccacgatcgg cagccggatc tgcaggttgg
     7561 cgatcgactc gcgcttcgcg gcgccggtga gctgcagcgc catgaggaag tcggaagccg
     7621 cgcgcacgcc gcgtgcgaag ccgttgggga tggtctcgag caccgtgggc cggccggcgc
     7681 cggcacacag cgcgatgtgg tcgaacccgt cggcgaacgc ctcgtcgatg gtgagcgtgc
     7741 cgccgaagcg cacgccgccg aacatcgcga actgcgcgcg ccgctccaga agcagccgga
     7801 tgatcttgag aaagttcttg tcccagcgca ccgtgatgcc gtactcggcc accccgccga
     7861 agcccgccat cacgcgctca tcgagcggct cgtagagttc cttcaggtcg cgcacggggc
     7921 cgaacggcac gcgccgtccc agcgcgtcga cgccggaaag cgcggcctcc agcggctcga
     7981 tcttcaaccc gtcgatgccg accacggtat ggccgtcgtt catcaagtgg tgcgcgatgg
     8041 agaagccggc cggccccagc ccgaccacca gcacgcgccg cccgctcggc gcccgcggat
     8101 acgggcggcg caggtcgagc ggattccagc gcgtcaggag cgcgtagatc tcgaaacccc
     8161 agggcagctc aaggacgtcc ttcagcgtgc gcgtctcagc ctgcgggatg tcgaccggat
     8221 cctgcttctg gtagatgcag gccttcatgc aatcgttgca gatgcggtgg ccggtggccg
     8281 cggcgagcgg gttgtcgacg gcgatcatgg cgagcgcgcc gatcggctgg ccgcgtgcct
     8341 tcaccacgtg gaattcggag atcttctcct cgagcggaca gccggcgagc tcgaccccga
     8401 acaccgtctt cttgaacccg cccgcgggtg ccttctcctt gaggcccttc gcgcaggagt
     8461 ccttgccctg ctcgtggcac cagatgcagt agttcgcctg gtcgagcgcg ccggcgaggt
     8521 ccgtgccgga atcggtgagc gcgaagccct ggcgatggcg gaattcgggc tgcgccggaa
     8581 cgaggcgcat gaggtcgagc ttgtgcggcg ccttgaacag cacgccggcc ttgtgctttg
     8641 ccttgccttc ctgcgtaccc gccgcccagg ccgcgtagcg cgccgccccc tccagctcgg
     8701 cagcgtgcgc cgcttcgtct ttctgccacg ccgtgatttg gcgcgcgaac tcgagctcgg
     8761 tggtgaagcg atagccgtcg gggcgcgcgt cctcgggctt gaccttgtgc agcgcgcgcc
     8821 gctgcacgaa caggcgcttc acgctgtaga gcggggcgag ctcgtggtgc cgcgcggcga
     8881 gcgcctgcag ctcggtctcg acgccgaaca ggcgcgcgac gaagtcctcc acgtgcggcg
     8941 caagcgcgat gagcagctcg gattcctcgc gcgcgggcgg cggcgcggcg cgggcttgct
     9001 cgaggcgctt gcacagctcg gcatccattt ccgcaacaaa ttgcagaaac gcggcgtcga
     9061 gccgcatgag cccgtcgcgg ctatagaggt cctcgaaggc aaggtcgaag cgcaggtgca
     9121 gcatcggcaa gtgcgtgaat tataatcggc gctctcatcg cgacgaggcg catatgctta
     9181 tcaagaacag cgtgtttctg gtcaccggtg gcgcatcggg tctcggcgcc gccaccgcgc
     9241 gcatggcagc ggagaacggc gccaaggtcg tcatcgcgga catgcaggtc gaggcaggcg
     9301 aaaagctggc gcaggagctc ggcgggcgct tcgtgaagtg cgacgtcacc gcggaagccg
     9361 acgccaaggc ggcggtggcg ctggcgctca aggagttcgg cggcctgcag gtgctggtga
     9421 actgcgccgg catcgcgctc gccgagcgca ccatcggcaa ggaggcgccg cacgatctcg
     9481 cgcgcttcac gcgcgtgatc accgtgaacc tgatcggcac cttcaacatg atccgcgtcg
     9541 cggccgatgc catggccaag gccggcccca acgcggcggg cgaacgcggc gtgatcatca
     9601 atacggcctc ggtcgcagcc tacgacgggc agatcgggca ggccgcctat tccgcgtcga
     9661 aaggcggtgt ggtcggcatg accttgccga ttgcgcgcga tctttcgcga aacggcatcc
     9721 gcgtgtgcac catcgcgcca ggcattttcg agacgccgat gctgctcggg ctgccgaagg
     9781 aggcgcagga ctcgctcggc aagcaggtgc cgtttccctc gcgcctgggc aggcccgcgg
     9841 agtacgcgca gctcgcacgc gccgtcatcg agaacgaaat gctgaacggc gaaaccattc
     9901 gcctggacgg cgccatccgc atggcgccga agtgattctg ccggaggcct agacctggct
     9961 gcgggacagg cgctcccgca gcatccgatg ctggatcttg cccgtcgcgg tccgaggcat
    10021 gtccgcctcc cgcaggaagg aaaccgaccg cggccgcttg aagccagcca ttttgtcgcg
    10081 gcaccaatcg aggatggcgg cctcggtcgc cgccacctcc tcccgcagca cgactacggc
    10141 gtgcaccgcc tcgccccatt tctcgtgcgg tacgccgatc acggccacgt ccttgaccgc
    10201 gggatggcgg ccgaggatgt cctcgatctc gctggggtac acgttctcgc cgccgctgat
    10261 gatcatgttg ctcttgcggt cgacgaggta aacgaaccct ttcccgtcgc ggcgcgccat
    10321 gtcgcccacg ctgcagtagc cgtccggacg gagcgcctcc gcggtcttgg caggatcgtc
    10381 aaggtagccg tcgaaggcgt acggggtgag cgagaacagc tcgccgacct cgccgtcggg
    10441 caggtcgcgg ccctcggcat cgagaatgcg cactgggccc gtgccggtcc actctcgacc
    10501 gaccgagccc aggtgctcta gctgctcgtg cggccgcaat agcgtgaccc agccggcctc
    10561 ggtggagccg tagagctcgt agagctgcga gttgcggaag tactccagga tggcgagctt
    10621 ggtgtccttg cgcgcgggcg ccgaggagat catcagcttg cgcacgcgcc ctacgtcgta
    10681 gcgcgccttc gtggcggccg gcagggcgag cgtcatgatg tagtgcgtcg ggacgagcga
    10741 ggtgaaggtc accccctcgg cggcgatcgt cgcgagcagc gcctcgggat cgaaactcgc
    10801 gcggtcgtcg atgatgcagg gcgcgcccag atacgtgaat gtgaaagcga agtagagcga
    10861 gttcgcgtgg cacatgggca tgacgagcag cgccttgtcc gcgcgcgtca agcccatgtc
    10921 gagcgccgta accagtgaga tgagcgcgct gccctggtgg ttgcggatgg cgcccttcgg
    10981 acggccggtg gtgcccgagg tgtacatcag cgcccagggg tcaagcggac gcacatccgc
    11041 ctgcggctcg cgatcagatg cggcggcgag cagcgcctcg tatccggtcc agcccgctgc
    11101 ctcgccgtca ccgaggcgga tccagccgcc cctcaccgcc ggcagctctc cgcgcaccgg
    11161 ttccacccgg tcgatgaggc tgtcctcagc gatgaacgcg cgggcgccgc aatgggcgac
    11221 gatgtagcgg atctcgggag cgagcaggcg aaagttcacc ggcaccgcgg tcaatcccgc
    11281 cttggcgagg ccgacgtaga tttccatcca ctcgagtcga ttgaaggcga ggaccgccac
    11341 ccggtcgccc ttgcgcaggc cgagaccggc caacgcgttg gccaggcggc aggcgcgcgc
    11401 attccactgg gcgaacgtaa tcgagcgccg ggagtcgcgt gcgcctaggg catcgggctg
    11461 cagccgcgcg tgcgtcgcga caacctggcc gaggtgcagc gtccctcgct gcagcgtgcc
    11521 gctcacgccc ggcgggcctt ctccgcttcg tcccaggcgg tgagcgcctg gtagtcgggc
    11581 acccagccca ggcccttgcc ggcgtcggag ctgagcgcat gctgcgtgac caccacgtcg
    11641 atgagcgccg gggctttggc gagcgcctcg gcaatggcgc cctccagccg gtccgcatcg
    11701 gtcacgcgct cggcatgcag cccgaacgcg cgcgccatgg cggcgtaatc gctccaggcg
    11761 agggtggtgc ccaccacccg tccgccgtaa ttgagctcct ggtcgaggcg ctcgatgttc
    11821 caggccgcgt tattggcgat gatgaagacc gccccggcgc cgtgccgctt cgccgtgtcg
    11881 acttccatcg cgttgatgcc gaacgcgcca tcgccgctca cgaccacgac ttgccggccg
    11941 ggatggagca gggcggcggc gttgccgtag ggaatgccga caccgaggca gccgaaggtg
    12001 cccgggtcga ggtacgtgga cggtgcttct aggccgaggc gcgcgaagct gagtatgtcg
    12061 ccgccgtcgg cgatggttat ggcatcgggc gcgaggagct tgcgcagggc ggcgaagatc
    12121 cggttcggat gcatgtgacc gtctttgccc gccggcgccg tggcgaggct ggcggcgtac
    12181 ttggacgagc gtcgctcgtg ctcttcgcgc aggccccgcg tccaggcctt gtcgagcgcg
    12241 ccggctgatg tcagcgccgc agcgatcgcg gacagcgcga gtcccggatt ggcgaacagc
    12301 tccacctcgc cgcgccggtt ttcgcgcagc tcctcggcgt tgtcggcgat gcggatccag
    12361 cgcgcgttcg cgaacacggc gggcgagccg taggccatct gatagtcgag cttgcggccg
    12421 acggtgacga cgaggtccgc ttcctgcatc gcgcgggcgc gcaccgcgcc gacgaacgag
    12481 ggatggctcc ccggtacgag gccgcggctc tcctgcgtgt cgaggtagac cgcaccgcac
    12541 gcctccaaaa gggcacgcag gggcggcgcg gtgccgagcg cgcccctgcc ggtgaccacc
    12601 agcggccggc gtgcggcgcg cagcagggcc gcggcgcgcc ccacgtccgc cgaatcagga
    12661 ggaacatggc gcacccgcct gcgcgagagg tactcgggga gcgcgagacg gggcgggatc
    12721 ttcaggcgga gcacgttcgt tgggatttcc aggtaaaccg gtcccggcgg gttgccgtcg
    12781 cccagcgccg tagaccagcc cttgtcgagg tcgcgcagga tgttgtccgg ggcacggagc
    12841 gtgcgtgcgc tgcgcgtgac cggccgcagg atcgccacgt gatcgatgcc ttgaagcggg
    12901 ccgaggtcgt cctgctgcac cggcgcacag ccgccgatga gcagcaccgg tacgcgctcg
    12961 agctgcgcgt tggcgacggc ggtgacgcag ttggtgacgc cagggccggc ggtggcgagc
    13021 gcgacaccga cctgtccggt cagcaccgcg tggcagtgtg ccatgtgcac ggcggcgcct
    13081 tcgtcgcgca cgtcgatgat gcgcacgccg cgctgtgcga gccagtccca gatcggctgg
    13141 atgtgcccgc cctgcaagcc gtacactcgg tccacgccct gggcgacgag gaactcgacg
    13201 atccactcgg cgaccgtcct ggctgtgctc atcatgtcct cctaacctgc ggcgctattg
    13261 aggcgcgggc atcggctgtt cgcgcatcgc ggctgcgtag aacttcgagc gctcgacgta
    13321 gacctgcgag gcgtgccgca gcatctctcg ctccgcgggc ctgagctcgc gctgcacccg
    13381 gccgggcgag cccacgacaa gcacgccggg cggaatctcc ttgccctcgg gcaccagcgc
    13441 tccggcgccg atcagggctc cggcgccgat cttcgcgccg ttcagcacga cggcgttgat
    13501 accgatcagc gcgccgtcgc cgatcaaaca gccgtgcagc acggcgcggt gaccgacggt
    13561 cacgttcgcc ccgatcgcga gcggaaagcc cgggtcgacg tgcaggacgg cgccctcctg
    13621 cacgttcgtg cgttcgccga tggtgatcgc gtcgttctcc gcgcggatca cgaccttgaa
    13681 ccaaatgtta acgtcggact ggagcgtgat gtcgccgacg agcgtggcgt cgtaggcgat
    13741 gaaatggcct gcgccggcga gcttcacgcg cttctcgcct atggagaaga tgggcatgca
    13801 gctatcataa tgcaatgcga atcgtggttc tcggcgccgg cgttatcggc gtaacaacgg
    13861 cgtggtacct ggcgcgggac gggcacaagg tgacgctcgt cgaccggcaa gccgaggccg
    13921 gcatggaaac ttccttcgcc aacggcggcc aggtgacggc cgggcatgcc gagccctggg
    13981 ccaatccgtc ggcgctgccg aaggtgctgc gctggctcgg gcgcgaggac gcgccgctgc
    14041 tgttccggcc gcgtgccgac cgggcgcagt ggctgtgggc gctgcgtttt gcgctggaat
    14101 gcctgcccgg gcgcttcgcg cgcaacacgc gccagctcgc cggcctcgcg cgctacagcc
    14161 gggactgcct cgccgagctg cgcgcacaga ccggcatcga gtacgagcgc gtcgcgcgcg
    14221 gcatcctgca tttctgcacc gagcacgcag atttcgaggc gctggcaaag cacgcggagg
    14281 acatgcgccg cctgggcatc gagcgcgagg tcaagtcggc ggcccagtgc cacgatctcg
    14341 agccggcgct gcgcgcgtcg caggcgaggg tggtgggcgg cgtctacacg ccgcaggacg
    14401 agtccggcga tgcgcatgaa ttcaccgtgc gcctcgcgca actggccgag caaagcggcg
    14461 tgcgctttct gcgccgggcg acggtggaat cgctcgacgt cgaaggcggg gccgtgagcg
    14521 cggtgcgtgc cggcggcgag cggctgcagg ccgacgcggt cgtggtctcg ctgggaagct
    14581 acagcgcgcg tctgctcgcc ccactgggca tctcgatccc ggtctatccg ctcaaggggt
    14641 actcggtgac cctgactctg ccgcccgagg tggcgacgca ggcgccgacg atcagtctca
    14701 ccgacgaggg gcgcaagctc gtgatttcgc gcttcggcgc gcgcctgcgg tgcgctggga
    14761 ccgcggagct gaccggctac gacacccgca tcaacgcaaa gcgctgcgaa gcgattgtcg
    14821 cccgcctgcg cgtattgttc ccggcgctgg cgcgcgccgg ggccgccgag ctctgggccg
    14881 ggctgcggcc cgcgacgcca agcaacgtgc cggtgatcgg tcgcacgcgt ctagccaatc
    14941 tgtatctcaa caccggccac ggcacgctcg gctggacgct ggcggcgggc tccggccgcg
    15001 cgcttgccga tcttctcgcc gggcgcaggc ccgacgtgga tttcgcgttc cgcggcgcat
    15061 gaaagcgagc gcatcgctct attacgacgt gcgcggcctg cgctatcacg tgcggcactg
    15121 gccgggcgag ggcgggccgc gcgtgctgct gctgcatggc tggatggacg tctcggcctc
    15181 gttccagttt ctggtggacg cgttgcccgc tcactgggag gtcttcgcgc ccgactggcg
    15241 tggctacggc ctcaccgatt ggggcaagtc cgattgctac tggtttcccg actacctggc
    15301 cgacctcgac gcgctgctac agcaactgtc gccgcaggcg ccggtcgatc tcgtcggcca
    15361 cagcctcggc ggaaacgtcg cctgcctgta cgccggagtg cggccctcgc gcatccgccg
    15421 gctggcgaat ctggaaggct tcggcatggc ggtgacgcgg ccagatcagg cgccggcccg
    15481 ctacgcgcgc tggctcgacg agctgggcag cagcaagggc ttccgccctt acgcgaactt
    15541 cgcggcgctt gccgagcgca tgcgccagag caatccgcgg ctcgcgcccg agcgcgcgga
    15601 gttcctctcg cgccactggg gacgcgagct gcccgacggc caggtgcagc tgcgcggcga
    15661 tcccgcgcac aagatcgtct acccggtgct gtaccgctac gaggaagcgc gcgcctgctg
    15721 gagcgaggtc acggcgccgg tgctgtgggt cgatgctgcc gagtcgaaga gcctcggccg
    15781 cgtcggcctc gacgccgcgc gctacgccga gcggcgggcg gcgttccgca acctgcgcta
    15841 cgttacggtg tccaacgcgg gccacatgct gcatcacgat caaccggagc agctcgcgcg
    15901 gctcctggag gagtttttcg gcgccccatg aatatcgatc tgcattgcca ttccaacgtc
    15961 tccgacggcg ccctcgcgcc cggggaggtg gcgcggcgcg cggcacgggc cggcgtcgcc
    16021 gtctgggcgc tgaccgacca cgatcagctt gccgggctcg ccgaggcgcg cgccgcggcg
    16081 cgggacgagg gcatgcgctt cgtcgacgga gtcgagatct cggtgacctg gcggggcgcc
    16141 accgtgcacg tggtcggcct gcgtatcgat cctggcaacg ccgatctggc cgcgggcctt
    16201 gccgccgtgc gcggcggccg gctgcagcgc gcgcgcgcga tggcgcacga tctgcagcgg
    16261 gccggcatcg acggtaccta cgaaggtgcg atcgagcgcg ccgagaatcc ggcgatggtg
    16321 agccgcacgc acttcgcgcg ctttctcgtg gacagcgggg tggtggccga catgcgcgaa
    16381 gcttttcgcc ggtttctcgt accgggcaag ccgggctacg tgccgcatca gtgggccgag
    16441 ctcgccgacg cggtgcgctg gatcggcgcg gcgggcgggc aggcggtgct cgctcacccg
    16501 gggcgctacg gcctgtccgc gggggcgatg gacgcgctgc tgcaggaatt ccgcgccgca
    16561 gggggcgagg cgatcgaagt ggtcacgggc agccattccg cggaacaaag ccggcatttc
    16621 gccgcgctcg ccgcgcagta cgacttctcg gcctcgcgcg gctcggattt ccacgggccc
    16681 gacgagggcg cggagttcgg cacgctgccg ccactcgatc cggcgctgcg gccggtgtgg
    16741 cgggagtggg gactgtagaa gccgcgcgtg gcgcagtact tcgccgtcca cccgacccat
    16801 ccgcagccgc ggctcatccg gcaggccgcg gacatcgtgc gcggcggcgg actgatcgcc
    16861 tatccgacgg actcttgcta cgcgctgggg tgccacctgg gcgacgcaga cgcgcaggcg
    16921 cgattgcggc gcgtgcgcgg catggacgac aagcaccacc tgacgctcat gtgccgcgac
    16981 ctttccgaga tcgcgaccta cgccatcttc gataacgcgc agtttcgact gctgaaggcg
    17041 gcgacgccgg gaagctacac gttcgtcctg cgcgcgaccc gcgaggtgcc gcgccgcctg
    17101 atgcacccgc ggcgcaagca catcggcgtg cgcgtgccgg cgcacccggc ggcacgcgcg
    17161 ttgctcgaag aaatgggcga gccgatgctc tcggcgacgc tgctgctccc gggcagcgcc
    17221 gtggcgctgt ccgatgcgca gcagatccgc gccgcgctcg aacaccagct cgacctcgtg
    17281 atcgatagcg gtccttgcgg caccgagccg agcacggtga tcgatctcac gggcgaagcg
    17341 ccggtggtgc tgcgcgcggg caggggctcg ctcgcggtgc tgggccagag cgtctgataa
    17401 gattcgcgac tgcccgatgg acgttaccca gctcgttcag acgctcgcga tctactcgct
    17461 cccagtgctg ttcgcgatca cgctgcacga ggcggcgcac ggctacgtgg cgcggtactt
    17521 cggcgacgtg actgcgtacg cgcagggccg catcagcttg aatccgttcc ggcatatcga
    17581 cctggtgggc acgatcatcg tgccgatcgc gatcctcgtc gccgccaagc tcgtcggcgg
    17641 cgccggcctg ctgtttggct gggccaagcc ggtgccggtg aattactcgg cgctcaggcg
    17701 accgaagcag aacatgctgt gggtcgcggc cgccggcccg ggcgccaacc tggcgatggc
    17761 gcttgcctgg gcgctgctgc tcaagctcac cgaagcgctg gcattgagcc aattctcggt
    17821 ggtcctgcgc ctggtctgcg tcgccggcat ccaggtgaac gtggtgctga tggtgctgaa
    17881 cctgctgccg atcctgccgc tagacggtgg gcgcatcctc gccagcctgc tgccgcaccg
    17941 catggcctgg caatacgcgc gcctcgagcc ctggggcttc ccgatcctgc tgctgctgct
    18001 gttctccggc tacctcggcg tggtgctgcg gcccgtggtc gaggcgtcgt acctggtgat
    18061 cggctcggtg ctccagctct aagcatgtac gagacgcgcg ttctctccgg catgcgcccg
    18121 accggcgcgt tgcacctcgg ccactaccac ggcgtgctca agaactgggt caggctgcag
    18181 gccgaatacc cctgcctgtt cttcgtcgcc gactggcacg cgctcaccac cgactacgag
    18241 caccccggac gcgtcgagca gaacgcctgg gagatggcgg tggactggct cgccgcgggc
    18301 gtcgacccga atcaggcgat cctgttcgtg caatcacaca ttcaggagca cgcggagctg
    18361 cacctgctgc tttcgatgat gacgccgctc ggctggcttg agcgcgtgcc gacgtacaag
    18421 gaccagatgg agaagctgaa ggaccgcgac ctggcgactt acggctttct cggctacccg
    18481 ctgctgcagt cggccgacat cctgatctac cgcgcgcgct acgtgccggt gggggaagac
    18541 caggtgccgc acgtcgagat gacgcgcgag atcgcgcggc gcttcaacca cctgtacggc
    18601 cgcgaggagg gcttcgaaga caaggcaaag tccgcggtga agaagatggg cacgaagaaa
    18661 gcgcgcctgc tgaacgagct gcgcaccaag ttcctcgagt ccggggacgc ggacgcgctg
    18721 aagcgcgcgc aggcgctgct cgaggagcag cagaacctgc cgcgcggcga ccgcgagcgg
    18781 ctgtatggct ggctcgaggg cggtggacgc aagatcctgg tcgagcccga ggccttgctc
    18841 accgaagcgg cgcggctgcc gggcctcgac ggacagaaaa tgtccaagtc ctacggcaat
    18901 gccatcggcc tgcgcgagtc gcccgaatcg gtcaccaaga agatccgcac catgcctacc
    18961 gatcccgcgc gcgtcacgcg gaaggatccg ggcacgcccg agaaatgccc ggtatggccc
    19021 ctgcacctgg tgtattcgga cacggcgcgc agggactggg tgcagaaggg ctgcaccacg
    19081 gccggcatcg gctgcctgga atgcaagcag ccggtgatcg aggcggtgct cgccgagctc
    19141 gcgccgatgc gcgagcgcgc ccaggtctac gtcgacgatc cgacgttggt aaagagcatc
    19201 ctcgccgacg gctgcgacaa ggcgcgcaag cttgcggtgg agacgatgcg cgacgtgcgc
    19261 gaatcgatgg gcctgaccta cacatgatcg agcccgcgcc gctggcgctc gctacccccg
    19321 tcgcccgcct ctacggcgag ccgctgatcg agattccgca ggacctgtac atcccgcccg
    19381 atgcgctgga ggtgatcctc gaggcgttcg agggaccgct cgatcttctg ctgtacctga
    19441 tccgcaagga aaatctcgac atcctcgaca tcccgatggc gccgctgacg cgccagtacc
    19501 tcgagtacgt ggaagcgatg cgcgccacca acctcgagct cgcggccgag tacctggtga
    19561 tggctgcgac gctcatggcg atcaagtcgc ggctgctgct gccgcggccc gctgccgccg
    19621 agccggagga ggaagacccg cgcgccgagc tggtgcggcg gcttctcgag tacgagcgca
    19681 tcaagaaagc cgctcaggcg ctcgacgagc tgccgcaggt cgggcgcgac gtgctcgcgg
    19741 tctcggtgtg ggtggagcgc acggtggccg agcgtctgcc ggaggtgaat ccgcaggacc
    19801 ttgccgaggc gtggcgcgcg ctgctgcaca gggcgcgcct gtcggcgcac catcacgtgt
    19861 cgcgcgagga gctctcggtg cgcgagcaca tgagcggcat tctgcggcgc ctgcgggagc
    19921 ggcgcgtgct ggagttcggc gagctgttcg acccggctcg gggtgtcgcg gtgctcgtgg
    19981 tgacgctgct cgcggtgctg gagctggcgc gcgagatgct gctcgagctt acgcaatccg
    20041 agagctttgc gccgatctac gtaaaattgg cccatgcaga acccctctga cgccaagcgg
    20101 atcctggagg cggcgctgct cgcttcgcag gagcccctgt cgatcgccga gctgcgccgg
    20161 ctgttcgacg atcagctcgc cgccgacacc ctgaagaacc tgctcgccga gctgcgcgaa
    20221 gactggagcg ggcgcgcggt ggagctcgca agcctcgcca gcggctggcg cttccagacg
    20281 cggcccgagt tccgtccgtt cgcggagaag ctgcagcccg agaagccgcc gcgctactcg
    20341 cgcgcggtga tggagacgct cgctatcatc gcctaccgcc agccggtgac gcgcggcgac
    20401 atcgaggaca tccgtggcgt gaccgtgtcg acgcagatca tccagacgct ggagagccgc
    20461 agctggatcg atacggtcgg tcaccgcgag acgcccggac ggccggcact ctacgccacc
    20521 acgaaaacct tcctcgacga cctcgggctg cgctcgctgc aggagctgcc gccgctcgag
    20581 gaaatcgcca agacgctgca gcttgaaccc gcgccggcgc ccgccgtccc cgaagccgcc
    20641 aacgaacccg ccgcaggctg agcgcctgca gaagctgctg gcggcggccg gcctcggctc
    20701 gcgccggcag gtcgagacct ggatagaggc cggacggctg agcgtcaacg gccgcaccgc
    20761 ccggctcgga gagcgcgcgc tcgcctcgga ccgcattgag ctggacggca aggctttgac
    20821 cctgaaagct tccggggcgc gaccgcgggt gctgctctat cacaagcccg atggcgagat
    20881 ggtgacgcgc gcggatcccg agggccggcc caccgtgttc cagcgcttgc cgccgatcga
    20941 gggcgggaaa tgggtcgcgg taggccggct ggacatcaat accgcgggct tgctgctgct
    21001 gaccgactcc ggcgagcttg ccaaccggct catgcacccg cgctacgaag tggagcgcga
    21061 gtacgcggtg cgcgtgctgg gggcgctgag cgaggagaat cggacccgac tgacgcgggg
    21121 cgtgcagttg gcagacggta ccggacggtt cgacagcctc gcgccctcgg gcggggccga
    21181 aggcgccaac cgctggtacc gggtcgtcct gcgcgagggc cgtaaccggg aggtacggcg
    21241 gctgttcgag gcggtgggca gcaaggtgag ccgcctgctg cgcgtgcgct acgggcccgt
    21301 ggccctgccg cgggacctgc gcccggggcg ctggatcgag ttggacgaaa aaagcgtcgc
    21361 ggagattgtg aaggcggcga aaagcggtta aacttcaagc cgtttcaggt agatgggctt
    21421 cggcccattt tttgtttgtg gccgcatccg tgccgaactc gctcgaaggc ctcttggagc
    21481 caaccctggc gggtctgggc ttcgaattgg tggacgcgca ggtgtccaac cgggggcgtt
    21541 tcgtgcgcat tttcatggac aagcccggcg gcatcacggt ggacgattgc gcggcggtca
    21601 gccgtcactt gtcccacctg ctcgaggtcg aaggcatcga ctacgaccgg ctcgaggtgt
    21661 cgtcgcccgg catggaccgg ccgctgcgac ggcccgcgga tttcgcgcgc ttcctcggcc
    21721 accgggtgga cgtgaggatg cggctggcgg acgcgacagg ccgacggcgc tatgtgggcg
    21781 tgctgcgcgg catcgacggg gccacggcga cggtggaagt ggacggcgcg ccggtggccc
    21841 tgcaactgga aggcatggat cgggcgcgac tcgttccgga actgtgagca tggagcaaag
    21901 atgagccggg aaattctgct gctggtggac gcgctggcgc gcgagaagaa cgtcaaccgc
    21961 gacgtcgtgt tcggcgcgct ggaaatggcg ctcgcctcgg ccaccaagaa gcgcttctcc
    22021 gaggacatcg acgtgcgcgt cgcgatcgac cgcaataccg gcgactacga ggctttccgc
    22081 cgctggcacg tggtcccgga cgacacgccc gagtggaaca cggcgcagat gctgtcgctc
    22141 gaggaagcgc aggaccgcaa ggccgacgcg cagatggacg agtacctcga gcagccgata
    22201 gagaagatcc cgttcggccg catcggcgca cagaccgcga agcaggtcat cctgcagcgc
    22261 atccgcgatg ccgagcgcga gcagatcctg aacgactttc tcggccgcgg cgacgagctg
    22321 gtcaccggat cggtgaagcg catggagcgg ggcaacgcca tcgtcgagtc cggccgcctg
    22381 gaagcgctgc tgccccgcga gcacatgatc cccaaggaga acctgcgcgt gggcgaccgg
    22441 gtgcgtgcct gggtgatgaa gatcgaccgc ggcgcgcgcg gcccacaact ggtgctgtcg
    22501 cgcactgcgc cgcagttcat catgaagctg ttcgagctcg aggtgccgga gatcgaagaa
    22561 gggctgctgg cgatcaagtc ggcggcgcgc gacccgggcg tgcgcgccaa gatcgcggtg
    22621 cacaccaatg accgcaggat cgaccccatc ggcacctgcg tgggcatgcg cggctcgcgc
    22681 gtgcaggcgg taacacagga gctcgccggc gagcgcgtcg acatcgtgct ctggtccgcc
    22741 gacccggcgc aattcgtgat cggggcgctg gcgcccgcgg aagtgagctc gatcctggtg
    22801 gacgaggaaa agcacgccat ggacgtggtg gtcgacgagg agaacctggc gatcgccatc
    22861 ggcaggagcg gccagaacgt gcgccttgcc tccgagctca cgggctggac catcaacctg
    22921 atgaccgagg aagaatcgac caagaagcag gccgaggagt ccacgcgcgt gcgcgagctg
    22981 ttcatgcagc gcctcgacgt cgacgaggag gtcgccgaca tcctgatcca ggagggcttc
    23041 tcgacgctcg aggaagtggc ctacgtgccg atcaatgaga tgatggaaat cgagtccttc
    23101 gacgagaata cggtgaacga gctgcgcagc cgcgcgcgca atgcgctgct ggtgcaggcc
    23161 atcgccagcg aggagaaggt ggaaggcgtg gaccagaacc tgctgcagct cgaaggcatg
    23221 gacacgcagc ttgccgccaa gctcgccgag ggcggcgtga agacgcgcga cgacctcgcc
    23281 gacctggccg tggacgagct ctcggagatg accggcatcg aggaagagcg cgccaagggc
    23341 ttgatcatgg cggcgcgcgc gcactggttc gagcaggacg gcgccgcgca actcaaggag
    23401 gcgcagtaac gtggccatga gcagcgtcgc gcaattcgcc agtgaactga agatgccgcc
    23461 cgccgtgctg ctggagcagc tgcgcgcggc cggcgtggag aagcgcgcgc ccgacgattc
    23521 gctcaccgag agcgacaagg cacggctgct ggagtacctg cgcaagtcgc acggctcggt
    23581 cgagcccaag aacaagatca cgctgacccg caagcagacc agcgagatca tcaagaagtc
    23641 ggacacctcg gggcgcgcgc gcaccatcca ggtggaggtg cgcaagaagc gcgtgttcgt
    23701 gaagcgcgat cccgccgagc tggcggcaca ggagggcgcc gcggaggcgc cggcccccgc
    23761 accggccgcg ccggccccgg cgccggcccc ggccccgatc ccggcggcga cgccggcgat
    23821 cgatgaacgc gagcgccagg cgcgcgaaga cgaagcgcgg cgccacgagc agctctcggc
    23881 actgcaggcg gcggagctca aggaaaagca ggagcgcgag cggcgcgagg ccgagcagcg
    23941 cgccgccgaa cgcgaggcgc aggtggcgat cgagcgcgcg caggcgcagc cgggcgcggc
    24001 gccgcccgtc gagggcacgc tgcacgcgcc gaagaccgat gccaagcccg acaagaagcc
    24061 gaagaaggcg aagcccacga ccaccatgcg ggacgattcc gtgcgccggc gcaccatcaa
    24121 gacgcgcggc gacatgggtg gcggcgtcgc gggctggcac gcccggggcg gcgcacggca
    24181 ccgcggcgct gcgggcgccg agcagcagac gttcagccag cccgccgagc cggtcacgcg
    24241 cgagatcgcg gtgccggaga ccattgccgt gggcgagctg gcgcaccgca tgtcggtgaa
    24301 ggccgccgag gtgatcaagg tgctgatgaa gctcggcacc atggccacta tcaatcaggc
    24361 gctggaccag gaaacggcga tgatcgtggt cgaggaaatg ggccacaagg cgaaggcggc
    24421 gaagctggat gatcccgagg cctacctggc cgagtccgcg caggacgcgg cgcaggaggg
    24481 tgtcgcaggc gagccgcggc cgccggtagt cacggtgatg ggccacgtcg accatggcaa
    24541 gacgtcgctc ctcgattaca tccgccgcac caaggtggcc gcgggcgagg cgggcggcat
    24601 cacgcagcat atcggcgcct accacgtcga aacgccccgc ggcgtcatca cgttcctcga
    24661 tacgcccggc catgaggcgt tcaccgcgat gcgcgcccgt ggcgcgatgg tcaccgacct
    24721 ggtgatcctg gtggtggccg cggacgatgg cgtcatgccc cagactgtcg aggcgatcaa
    24781 ccatgcgaag gcggcagaag tgccgctggt ggtggcgatc aacaagatcg acaagggcga
    24841 agccaacccg gagaaggtga agcaggaact gctgcaacac ggcgtggtgc cggaggagtt
    24901 tggcggcgag gtggcatttg tccccgtgtc ggcgaagacc ggcaaaggcg tggacgattt
    24961 gctcgagcgc gtgttgctgc aggccgaggt cctcgagctg aaggcgcccg cgcagacgcc
    25021 cgcgcgcggc gtggtgatcg aggcacggct ggacaagggc cgcggcccgg tggccaccgt
    25081 gctggtgcgc tcgggcacgc tgcaccgcgg cgacgtggtg ctgacgggcg ccgtctacgg
    25141 gcgggtgcgg gccatgctcg acgaaaacgg caagccgatc gagtcggcgg gcccgtcgat
    25201 cccggtggaa atccagggcc tgtcggaggt gccgacggcg ggtgaagaca tggccacgct
    25261 cgccgacgag cgcaaggcgc gcgagattgc gctgttccgc cagggcaagt tccgggacgt
    25321 caagctcgca aagcagcagg ctgccaagct tgagaacctg ttccagcaga tgggcgaggg
    25381 cgacaagaag gtcctgtcgc tggtgatcaa ggccgacgtg cagggctccc aagaggccct
    25441 ggtgcacgcg ctgacacggc tgtcgagcga ggaggtgaag gtcggggtgg tgcacgcggg
    25501 cgtcgggggc atcaccgagt cggacgtgaa cctggcgctc gccgcgaagg ccgtgatcat
    25561 cggcttcaat acgcgcgcgg atgcgactgc gcgcaagctg atcgaaagct ccggcatcga
    25621 ggtgcgctac tacaacatca tttacgaggc ggtggacgag atcaaggcgg cgctctccgg
    25681 catgctggcg ccggagcgca aggagcaggt gctcggcctg gtcgaggtgc gtcaggtgtt
    25741 ccgcatctcc aaggtcggcg cggtcgccgg ctgttacgtc gtcgatggtc tggtgcgccg
    25801 caatgcgcag gtgcgcgtgc tgcgcgacaa cgtcgtcatc cataccggtg agatcgactc
    25861 gctgaagcgc ttcaaggacg acacgcgcga ggtgaaggcc ggcttcgaat gcggcatgtc
    25921 gctgaagaat ttctcggaca tcaaggaagg cgaccagttc gaggtgttcg aagtcctcga
    25981 ggtggcgcga accctgtgag ccgcggacga ccgcaaaagg tcgccgacca gatccagcgc
    26041 gagctgtccc tcctcctgag ccgggagctg cgcgacccgc gcctcggcct ggtgaccctg
    26101 accgcggtgg acgtggcgcc ggacctgtcg cacgcggtgg tgtatttcac ctgcctcgac
    26161 gcggcgcagg tcgagcccgc gacgaccgcg ttgcagcacg ccgccggcta cctgcgcacg
    26221 cacctcgcga agcggatcaa gctgtacacc acgcccgagc tgcgctttcg ccacgacgag
    26281 tccgtggcgc gcggcgcgta tttgtcgcag ctcatcgaca gcgtccggcc aaagcgctga
    26341 tgcgccgccg gctcgacggc gtgctgttgc tcgacaagcc ccccggcgtc ggctcgaacg
    26401 ccgtgctgca gacggtgaag cgcctctacg gcgccgagaa ggcgggccac gccggcaccc
    26461 tcgatccgct cgcttcgggc ctgctgccgg tgctcttcgg ggaagcgaca aagttttcgc
    26521 agctcgcgct ggatgccgac aaggcataca tcgcgaccgc gcgcctaggc attaccacga
    26581 cgaccgggga cgccgagggc gaggtcctcg agcgcaagcc ttgcaacgta cgcgcggagg
    26641 acgttgagcg cgcgctggcc gcgttccgcg gcgaactcga gcaggtgccg cccatgtact
    26701 cggcgctcaa gcacggcggg caaccgcttt acgcgctggc gcgtgccggg cgcgaagtgc
    26761 cgcgcgcggc gcgccggatc cgcgtgcacg cgctggaact gctgcgcttc ggcggcgaca
    26821 gcctggagct gcgcgtgcgt tgctcgaagg gcacctatgt ccgcacgctg gtggaagaca
    26881 tcggccgcgc gctcggctgc ggcgcgcatg tgagcgcgct gcgacgcacc gcatcgggaa
    26941 tgtttcgcat cgaggacgca gtcacgctcg agcagctgca ggccgagatt cccggcgcgc
    27001 gcgacgcgag gctgcttccg ctcgagcggc ttctcggcgg gctgccgcgg gtggacctgc
    27061 ccgcggcgct cgccgagcgc ttcctgcgtg ggcaggcgct gcaggtcgag catgcgccat
    27121 cgggaccctg cggcgtctac ggccagcagg gccggctgct cggcctgggc gagggccggg
    27181 cacaggggga gctgcgcccg cgccggctcc tgagctacgc aagcggctga aaaacatcga
    27241 aaaaaacttg aagaaccggg ccgggctcgc tagaatgcgc ggcttaaact aggagacgaa
    27301 gagtatggct ttgcaggttg cgcagaaagc gcaggtcgtt cagcaatacc agcgatccaa
    27361 taaggatacc gggtcgcccg aggtacaggt cgcgctgctc acggagcgca tcaacagcct
    27421 ggccgagcat ttcaagacca atgtgaagga tttccattcc cgccgcgggc ttctcaagct
    27481 cgtgtcgcag cgccgcaagc tgctcgatta cctgcggcgc acggatgccg acaagtaccg
    27541 ctcgctcatc gagcggctca gtctgcgcaa gtagcacgca tgccgccagc agcgacgctc
    27601 atcccgcgat gagcgttttt gcttttggcc accacacgag gtaacccatt gaatcccacc
    27661 aagaagacat tcacgctggg tgaccagcaa gtaacgttcg aaaccggcga gatcgcgcgc
    27721 caggcgcacg gcgccgtcct ttgcaccatc gatgacaccg tggtgctcgc caccgtggtc
    27781 ggcgcgaagg aaccgaaggc gggccaggac tggtttccgc tcaccgtcga ctacgccgag
    27841 cgtacctacg ccgccggcaa gatcccgggc ggcttcttca agcgcgaagg ccgaccctcc
    27901 gagaaggaaa ccctgacctc gcgcctgatc gaccggccga tccggccgct gttcccggac
    27961 ggcttcttca acgaggtgca ggtggtggcg caggtgctgt cgctgaatcc ggaagtcgat
    28021 tccgacattc cggcgatgct ggcggtatcg gccgcgctga cgctgtcggg catcccctgg
    28081 aacggcccga tcggcgcgtg ccgcgttggc tacgtgaacg gccagtacgt gctcaatccg
    28141 tcggccacgc agctgaagga atccaagctc aacctcgtgg tggccggcac cgagcaaggg
    28201 gtgctgatgg tcgagtccga ggcgcacgag ctggccgagg acgtgatgct gggcgccgtg
    28261 gagttcggcc acgcgaagat gcaggccgcg atccaggcaa tcgacgagct ggccgaagtc
    28321 gccgccaagc ccgcctggga ctggcagccg ccggcgaaga acgaggcgct catccagcgc
    28381 atcgcggagc tggccgaagc cgacctgcgc gccgcgttcg agatgcggca gaagcaggcg
    28441 cgcaacacgc gcctgaacga gatccgcaag gcgaccctgg ccaaggtgga cgaggaggcc
    28501 aaggcggcgg gtaccgagct cgcgccgaat gtggcgaaag acgtgttctt caacctcgag
    28561 tcgaagatcg tgcgcaacca gatcctcgat ggcgagccgc gcatcgacgg gcgcgacacg
    28621 cgcaccgtgc gcccgatcaa tgtgcgcgtc ggcgtgctgc cgcgcacgca cggctcggcg
    28681 ctgttcacgc gcggcgagac ccaggcgctg gtcagcgtca cgctcggcac cgggcgtgac
    28741 gagcagatca tcgatgcgct ggccggggag taccgcgagc gcttcatgtt ccactacaac
    28801 atgccgccgt atgcgacggg cgagaccggg cgcttcggct tcaccaagcg ccgcgagatc
    28861 ggccatgggc ggctcgccaa gcgcgcgctg gtggcgatgc tgccgggcgc cgaggacttc
    28921 ggctactcgc tgcgcgtggt ttccgagatt accgagtcga acggctcctc gtcgatggcc
    28981 tcggtgtgtg gcggcagcct ggcgctgatg gacgccggcg taccgatcac ggcgcacgtg
    29041 gcaggcatcg ccatgggcct gatcaaagag ggcaaccgct tcgcggtgct ctcggacatc
    29101 ctcggcgacg aggatcacct tggcgacatg gacttcaagg tcgcgggcac cgagaccggc
    29161 gtgaccgccc tgcagatgga cctcaagatc gagtcgatca acaagagcat catgaagatc
    29221 gccctcgacc aggcgcgcga agcacgcctg cagatcctga ccatcatgaa gcaggcgatc
    29281 gagggctcgc gcaccgagct gtcgagcttt gcgccacgca tcatcaagat caagatcaat
    29341 ccggacaaga ttcgcgacgt catcggcaag ggtggtgtgg tcatccgcgg gctgcaggaa
    29401 gagaccggca ccaccatcga gatcgaggac gacggcacca tctcgatcgc ctgcgtctcc
    29461 gccgaaggcg gcgaagcggc gaagaagcgc atcctggaca tcactgcgga cgtcgaagtc
    29521 ggcaagatct acgaaggtcc ggtgctgcgc ctgctggatt tcggcgccat cgtgcagttg
    29581 ttgccgggac gcgatggcct gctgcacatc tcgcagatca gccacgagcg cgtgaacgcc
    29641 gtctccgatc acctgaagga agggcagatc gtcaaggtga aagtgctcga ggccgacgac
    29701 aagggacgcg tacgcctgtc gatgaaggcg atcggcgcgc cggaggcggc aaagcaggcg
    29761 taaaaaaggg gcgccgcggc gcccctcgtc ttgcgttgcc cgacggggtg gctacttggc
    29821 cacctgtttc tcgcgcagct cgtccagtgt cttgcagtcg atgcacagcg tggcggtggg
    29881 acgcgcctcg agccgcttca agccgatctc cacgccgcag ctctcgcagt agccatactc
    29941 gttggcgtcg atgcggccga tggtctcgtc gatcttcttg atgagcttgc gctcgcggtc
    30001 gcggttgcgc agctcgagcg ccatgtccga ttcctggctg gcgcggtcgt tcgggtcggc
    30061 gaacaccgtc gcttcgtcct gcatggtgtg caccgtgcgg tcgatctcct cggacagctc
    30121 ggctttcagc tgctcgagca tcttgcgaaa atgcgtgcgc attctcgcgt tcatgtagtc
    30181 ctcgcccttt ttcggcgcgt agggcgtgaa gctcttgcgg gtcagttcaa cagccatttc
    30241 gtggttcggc ccgaaaaaca aaggcgcctt tgtagcagaa aagcccccca gcggcaagcc
    30301 gaaaaaagag tatcatgagc gccctttgcg gggtgtggcg cagcctggta gcgcgcttgc
    30361 tttgggagca agatgtcggg agttcgaatc tctccacccc gaccagtttt gcccggcggc
    30421 tgcccgtagc tcaaccggat agagcaccag ccttctaagc tggg
//
LOCUS       DBNHKE010000138        15082 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_922882, whole genome shotgun sequence.
ACCESSION   DBNHKE010000138 DBNHKE010000000
VERSION     DBNHKE010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_922882"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="AC16O9_09210"
     CDS             complement(<1..1055)
                     /locus_tag="AC16O9_09210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR6516833.1"
                     /translation="MSPGEVPKSIAVLRMSALGDVVMTLPLLRTLQKAFPRTKIHWIV
                     SRPFDTIVQGISGIELVVIDKPRGPLSFWRLRRQMRAYDFDWLLVPQASMRANLVTLV
                     TRAKRKIGFGALHSRDLQRWFVNETVPAEPEHLIDSFLRFATYVGARERVLDWNLPLR
                     PEEHAWARAQVAACAGIKLAVVLAASKPERNWPVARYCETLERLAARWPLSIVLIGGP
                     GAAERALAGEVHRRVRIPVLDMVGRTDLRQLAALLGQADALLAPDTGPLHIAQAMGKP
                     VVGLYAVAPSAKTGPYLEPQWTVDVFAQAAATILGRERMGWRQRVHDERALRLITVDA
                     VVDRIERLFEHRGFRPQT"
     gene            complement(1063..2535)
                     /gene="tcuA"
                     /locus_tag="AC16O9_09215"
     CDS             complement(1063..2535)
                     /gene="tcuA"
                     /locus_tag="AC16O9_09215"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR6516834.1"
                     /translation="MSERYDVIVVGAGNAAFCAALAAKEKAASVLVLERSGEDEAGGN
                     SRFTAGLMRVAYAGVDDLKRAIPDLTAEEIERTDFGTYTEEQFLDDMARVTEFRCDPD
                     LTEILVKRSLATVEWMRQKGVRFTAAWGRQAFNIGGRFKFWGGLTVEAVGGGPGLVES
                     LTQAAKKNGIEIWYKARATGLIADDAGVKGVRVKKDGKTLEVRAKSVVLAAGGFQANP
                     EWRARYLGPGWELAKVRGTRFNTGDVIRMALDAGAAPAGNWSGCHAVAWERNAPEFGD
                     LAVGDQFQKHSYPWGVYINAQGKRFVDEGADFRNYTYAKYGRVILSQPGQFAWQIFDA
                     KVKAQLRDEYKIRQVTKVTAGTLEELCAKLEDTDGKTALEELKAYNAAVRQDVPFNPN
                     VKDGRGTQGLAIPKSNWANTLDTPPFEAYAVTCGITFTFGGLRINTDAQVLSTDGEPI
                     AGLYAAGELVGGIFYFNYPGGTGLTNGAVFGRIAGTSAAR"
     gene            2581..4248
                     /locus_tag="AC16O9_09220"
     CDS             2581..4248
                     /locus_tag="AC16O9_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6516835.1"
                     /translation="MTSDAAPDVIVLGAGAGGLTAACAAAARGLRVRLLEKTDRVGGT
                     AAISGGMVWIPGNSRMPGAGLADSEAAVRAYLNATLSAGTRPALIDAFVREGPAAIDW
                     LEARTELRLKPVTRYPDYYPALPGATPGGRVLEPVGYDARALGADLALLRPPLPEFTL
                     FGGMMVDRADLPHFRAVTRSPRSMARIARLLARHARERLAAPRGTSLVLGNALIARLL
                     RSARLLGVEIRTRAEVAALSQENGHVRGVVLGDSTRLEARRGVVLATGGFSHDAERRA
                     RWLPRGTDVASAVCDGDAGDGLRLGESAGGRVAEGNLHNAYWAPISKFRRADGSAAVF
                     PHTVADRGKPGIIAVDAQGERFTSEAVSYHEFALAMFRARAIPAFLLCDRRSLWQYGL
                     GAVRPFTLRLARLLEQNYLASGATPGALADRLGVDRAGLDRTVAQYNADAVRGVDSRF
                     NLGGDVYQRFLGDAAHGPNPCMRPIEVPPFFAVRLYPSDLGTAAGLRTDENARVLDGD
                     GRPIAGLYACGNDMNSIMGGAYPGPGITLGPALVFGWLAARHLADCA"
     gene            complement(4250..6055)
                     /locus_tag="AC16O9_09225"
     CDS             complement(4250..6055)
                     /locus_tag="AC16O9_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6516836.1"
                     /translation="MARMKGGELIAEYLIQQKVPYIFGICGHGNVGILDPLYAVRDKL
                     KLVSPRHEQCAGHMADGYFRVKHQPVATLTSTGPGSANLVMSLATALSDSSAFLAITS
                     NVPTSQMNRAPFQEIYKHNQADFPSVIRPVVKRAFQPTRVDMLPLALRQGFDTMVTGR
                     PGPVNIDVPYNVYQEEDEVELPPAAHVFGQHRPGASDADIARAVNLLTDATRPVLFIG
                     HGATLAEAGPEITQLQQTYGIPVITSPNGMGCVPADDPLTLGFIGRNGAYPANQAGRH
                     ADLVVTIGTRFDDRSSSSWHPGYSWNFPKTKLIHVDLDPQELGRNYPPTLGIIADAKV
                     FTAQLLQALGLRESRGAPPLKKQLSEWRAEVEGWRKEWEAYVRPNFGKLTTPLRPEYV
                     VDALQKVLPDDAILTCDSGVHHNWFMQFWRPKRAQSMLNSWGYSSMGFGVCSVMGAQL
                     AAPERPCISVCGDGGFSMTPYVVATAVEYGLPCIWLVWNNFSWAAIRDIQVGMFGGRE
                     LGTAFYPGGHYGQTQGKKYNPDFAAWARACGADGVTITKSEDLSGALQQAIRNKRPCV
                     LDVHVDAEVRPPSTGTWELPPTPYKEPAFGKPWVA"
     gene            complement(6059..6448)
                     /locus_tag="AC16O9_09230"
     CDS             complement(6059..6448)
                     /locus_tag="AC16O9_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6516837.1"
                     /translation="MNRKQVRGTAAFVEQMARVAWREFPGHFGGALSKMLVGPEKGGS
                     ARVDFRISRYAPNAYVEEHSHRVQEQIYYVIEGEGVLTVDGEEHLMRPHDYVYLPPGV
                     RHAITNTGTGGLVFFVVTTPASDEAAV"
     gene            complement(6471..7211)
                     /locus_tag="AC16O9_09235"
     CDS             complement(6471..7211)
                     /locus_tag="AC16O9_09235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516838.1"
                     /translation="MELELSGKTALVTGASAGIGRGIALALAAEGVRLAVTARRRERL
                     EALEKETGAKLVIIEQDFMAEGAPQKIADAALNGLGAVDILVNNAGGSRAFKLDSSEA
                     QWEEAMTLNFTRQRQLAHALLGQMMARRWGRVVNITGKSEPEGLNGAFAAKAAMHAWA
                     KGLSIEVGKHGITVNCIPPGRIMSEQIERNYTPEFRKWQAEHEIPVREYGQPADIANL
                     VCFLASPRARYITGAVIPVDGGLRKYQF"
     gene            complement(7218..8801)
                     /locus_tag="AC16O9_09240"
     CDS             complement(7218..8801)
                     /locus_tag="AC16O9_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6516839.1"
                     /translation="MNVSEVMVRYLKAAGIGHVFGYPGDPNIEFMEAARRAGLEFVLG
                     RREGTAGLMAEAYGFLTGRPGVCMSTLGPGSSNLVNAVANAYLDRVPMIALSGQIERK
                     RAQTFTHQVLDHNRLFSPVSKWTTEIAPDTVAGVMRRALRTATAERPGPVHITTHADV
                     VGAEAQDDKVALPPLAVTAFGGDGGVARRLAKAKKPVILAGIAAQRANAHAALAGLAE
                     KAGIPVVVAPMAKGVLAEDHAYYAGTLDMACNALMWEFLRGCDLLVAIGFDAVELIKP
                     WTLSVPAVHIDAVPNTDQIYSADTELVGDIAAIVGAIARAWKGEARWKAAAVKRHRDA
                     LRAAYYAGRVEGKLNPTDVIDAVRAALPRAAIATTDVGSHKLLVGQGWTTTAPRGVLM
                     SNGLSSMGYSLPAAIVAQLLHPKQPVVCFVGDGGLAMVQGELRLAASLKLPLLVVVFC
                     DGSLNRIEIKQANRKYPSWGTLIEATDIVQLARAMRCEGARVDSAQALERLLAQKRPK
                     DRPLVVGADIDPAQYTQQF"
     gene            8848..9186
                     /locus_tag="AC16O9_09245"
     CDS             8848..9186
                     /locus_tag="AC16O9_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6516840.1"
                     /translation="MPRGVVDLEGLKSPPAEALRRMAPPAGLPFQVRKLGHVVLKVKD
                     VKRSAAFYTGVLGFRVSDVYPESMKKGGTVFLRCNADHHCLEMFWGLDQVGSDDRSRP
                     PGPDTTLASK"
     gene            9183..10058
                     /locus_tag="AC16O9_09250"
     CDS             9183..10058
                     /locus_tag="AC16O9_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6516841.1"
                     /translation="MSESIAFIGFGEAGQTISRGLAKEAAQPALRAYDILFGAPAGAK
                     LEAAAMALGVGLARDHVEAVREAGLVILVVTASSSLEATKSCLPGLRKGQLFLDMNSV
                     SPKRKLETAALVAPTGAAYVDCAVMAPAAPYLHKVPCLVGGPGAAALAPRAAALGMKM
                     ELVSDEVGQASAIKMFRSIVVKGLEALLVESMTACAEYGVEDRVLASLKETWPGIDWQ
                     KLSGYMIERVVTHGRRRAAEMREVSETLQSIGIDPLMAAATAERQQWVADLGVKASSQ
                     DRAELVGVIRKAMGK"
     gene            10055..10852
                     /locus_tag="AC16O9_09255"
     CDS             10055..10852
                     /locus_tag="AC16O9_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6516842.1"
                     /translation="MKALLGEYPCTALFRQRAPLAFEDVKPPSAGFKRVVRNLEFDFA
                     ELAITTFLMAKAAGKPYRLLPAVVLARMQHPRLVCDRTRPVAPRTLEGRRVAVRSYSV
                     TTGMWIRGILAEDHGVDHRRITWVTFEEAHVAEFRDPPNVERAPAGRKPEEMLLAGDV
                     DAAVLGDAPVTDPRIVPVIPDAEAAAREWRARKGAIQINHMVAIKPHVDAAPLMRLLE
                     ESFAAAGNPEMNPIGLDANRRNLEVAVDFVHRQGLVPQRYAVEELLA"
     gene            10849..11841
                     /locus_tag="AC16O9_09260"
     CDS             10849..11841
                     /locus_tag="AC16O9_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6516843.1"
                     /translation="MIIDCHGHYTTAPQALWDWRSKQVAGQDPGPLRISDDEIRETLE
                     QNQLRIQRERGTDLTFFSPRAGSMEHHVGDAKTSEAWSRQCNDLIHRVCRLYPQNFAA
                     VCQLPQSPGVPPENCIPELERCVEELGFIGCNLNPDPSGGWWKAPPLGDRYWYPLYEK
                     LVALDVPAMVHVSSSCNPAFHFTGAHYINADTTAFMQFIQSDPFKDFPTLRFVIPHGG
                     GAVPYHWGRYRGLAQDMGKPPLTELLLRNVFFDTCVYHLPGIELLLKVIPVDNILFAS
                     EIVGAVRGVDPQTGQYYDDTRRYIDAVGSLSAADREKIYSANVRRVYPRFARRA"
     gene            11879..13081
                     /locus_tag="AC16O9_09265"
     CDS             11879..13081
                     /locus_tag="AC16O9_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6516844.1"
                     /translation="MTTTSSRWLWRFVVVLILAAGVGGFAALNALRPEPAVRAPTTQL
                     PLVQAAPLEFRDGALRVTGHGLVKARAEAVLGVEVPGRLVFVSPSLAAGGAFTRGEVL
                     LRLDDEPYRAALAQAEAELASARAALALGEQQLERTRELIAQGFQSRQALDERSSSRD
                     QAAAAFARAEALVRQRRVDFERAVVRAPFDGKVLSARVVRGESVQPGKELARFYADGV
                     LEIAVSLTDREMALAADPWRTGARGRGAAASVEVEHGGGVYRWPAHVDRVESSVDAAT
                     RTFNVIVRVDNPAERGSAVRGNAAAAPPLLVGMYATVHIVGIDPGRHALVPRRALREG
                     DALWLLQPGGVLNVRPVRVLQTSGERVAVSAEGLPEGARVVVSDLKVVTDGMRVRELG
                     AGERRASP"
     gene            13078..>15082
                     /locus_tag="AC16O9_09270"
     CDS             13078..>15082
                     /locus_tag="AC16O9_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6516845.1"
                     /translation="MRGLIAWMARHPVAANLLMLLIVVAGLSSALTMKQEVFPLVELD
                     VIEVRVAYPGAAPDEVEEAIVQKIEEQVEGIDGVDRVTSVAAEGTGVVRIELARGVQP
                     TVKLDEVKAAVDRITTFPDEAERPEVFQLVTRQRVVELAVFGEASEATLRELAYRIKD
                     DLTATPGVSLAQVARVRPYEISVEVPNDVLRAYGLTLQDVAATLKRASLDLPAGDIEA
                     SGESILLRARGRNYNRADFERVIVVSSASGAQVRLGDIARIDDGFRDDELIMRFNGRP
                     AAFVQVFRVGEDKVLDVVAKVNDYLDTTLRPGLPPGLEVIVWRDDSAEFRNRRDLLVK
                     NGVLGLALVLAALALFLDLRLALWVAAGIAVSFIGTFAVMPWLGLSINMMSLFGFILA
                     IGIVVDDAIVTGENIYAENERGTPEMDAAVRGSQRVVLPVALAVSTTIAAFVPLLFAP
                     GTLGKFLFQIPAVVIIVLAVSVLEAMLILPHHLAHLRVIGYAPRTWIGARLADLRHAV
                     DRALRRFIEGPLDAALRFATAHYGVVIASAFSVFLLTVGAITAGHVRFVFFPQVEGRY
                     VTASLELAPGATAETTLAAAEDIARAGREAAVSLPDPSGRDLVSAVYITVGRQEAAGP
                     GAVGALSLLQGNKASVVFELLDPEVRSIASGDFERRWRDLAGQP"
BASE COUNT         2062 a         5389 c         5301 g         2330 t
ORIGIN      
        1 gtctgcggcc tgaagccgcg gtgctcgaaa agtcgctcta ttctatccac caccgcgtcc
       61 accgtaatga gccgcagcgc gcgctcgtcg tgcacgcgct ggcgccaccc catgcgctcg
      121 cgtcccagga tcgtcgccgc ggcctgcgcg aacacgtcca cggtccattg cggctcgaga
      181 tacggcccgg tcttcgcgga cggcgccacc gcgtacagcc ccaccacggg cttgcccatc
      241 gcctgcgcga tgtgcagcgg cccggtgtcg ggcgcgagca gcgcgtccgc ctggccgagg
      301 agcgccgcga gctggcgcag gtcggtgcgc cccaccatgt cgaggaccgg gatgcgcacg
      361 cgccggtgca cctccccggc cagcgcgcgt tccgccgcgc ccgggccgcc gatgagcacg
      421 atgctgagcg gccagcgcgc cgcgagccgc tcgagcgtct cgcagtagcg cgccaccggc
      481 cagttgcgct cgggcttgct cgcggcgagc accacggcga gcttgatccc ggcgcatgcc
      541 gcgacctgcg cgcgcgccca ggcgtgctcc tccgggcgca gcggcaggtt ccagtcgagc
      601 acgcgctcgc gcgcgccgac gtaggtggcg aaccgcagga agctgtcgat gaggtgctcg
      661 ggctcggcgg gcaccgtctc gttcacgaac cagcgctgca ggtcgcgcga atgcagcgcg
      721 ccgaagccga tcttcctttt cgcgcgcgtc accagcgtga cgagattcgc gcgcatgctc
      781 gcctgcggca ccagcagcca gtcgaagtcg taggcgcgca tctgccgccg cagccgccag
      841 aacgagagcg ggccgcgcgg cttgtcgatg acgaccagct cgatgcccga gatgccctgc
      901 acgatcgtgt cgaacgggcg cgagacgatc cagtggatct tcgtgcgcgg gaaggccttc
      961 tgcagcgtgc gcagcagcgg cagcgtcatg acgacgtcgc cgagcgcgga catgcgcagg
     1021 acggcgatgc ttttcggcac ctccccgggg ctcatccccg gattatcgcg cggcgctggt
     1081 gcccgcgatg cgcccgaaca cggcgccgtt ggtgaggccg gtgccgcccg ggtagttgaa
     1141 gtagaagatg ccgccgacca gctctccggc cgcgtacagg cccgcgatcg gctcgccgtc
     1201 ggtggacagc acctgcgcgt cggtgttgat gcgcaggccg ccgaaggtga aggtgatgcc
     1261 gcaggtgacg gcgtaggcct cgaacggcgg cgtatcgagc gtgttggccc agttcgactt
     1321 cgggatcgcg agcccttgcg tgccgcggcc gtccttcacg ttcggattga acgggacgtc
     1381 ctggcgcacc gcggcgttgt acgccttcag ttcctcgagc gcggtcttgc cgtcggtgtc
     1441 ctcgagcttc gcgcacagct cttccagcgt gcctgcggtc actttggtga cctgccggat
     1501 cttgtactcg tcccgcagct gcgccttcac cttggcgtcg aagatctgcc aggcaaactg
     1561 cccgggctgc gaaaggatca cgcgcccgta cttggcgtag gtgtagttcc tgaagtccgc
     1621 accctcgtcg acgaagcgct tgccctgcgc gttgatgtac acgccccagg ggtaggagtg
     1681 cttctggaac tggtcgccca ccgcgaggtc gccgaactcg ggggcgtttc tttcccacgc
     1741 gacggcgtgg cagccggacc agttgccggc gggcgcggcg ccggcatcga gcgccatgcg
     1801 gatcacgtcg ccggtgttga agcgcgtgcc gcgaactttc gccagctccc agcccggccc
     1861 gaggtagcgc gcgcgccact cgggattggc ctggaaaccg cccgcggcga gcaccacgct
     1921 cttcgcgcgc acttcgagcg tcttgccgtc cttcttcacg cgcacgccct tcacgcccgc
     1981 gtcgtcggcg atcagcccgg tcgcgcgcgc cttgtaccag atctcgatgc cgttcttctt
     2041 cgccgcctgg gtgagggatt cgactaggcc ggggccgccg cccaccgctt ccaccgtgag
     2101 tccgccccag aacttgaagc ggccgccgat gttgaacgcc tggcggcccc aggcggcggt
     2161 gaagcgcacg cccttctggc gcatccattc gacggtggcg aggctcctct tcaccaggat
     2221 ctcggtgagg tccggatcgc agcggaactc cgtgacgcgc gccatgtcgt cgagaaactg
     2281 ctcctcggtg taggtgccga agtcggtgcg ctcgatttct tccgccgtga gatcgggaat
     2341 tgcgcgcttg aggtcgtcca cccccgcgta ggcgacgcgc atcaggcccg cggtgaagcg
     2401 cgagttgccg cccgcctcgt cctcgcccga gcgctcgagc accagcacgc ttgcggcctt
     2461 ctccttcgcg gcgagcgccg cgcagaacgc ggcgttgccc gcgcccacta cgatgacgtc
     2521 ataacgttcg ctcaagccac ttctccctta gtcgcaaatt cacgtataca gcatgttccg
     2581 atgacgagcg acgcggcgcc ggacgtgatc gtactgggcg cgggcgccgg cggcctgacg
     2641 gccgcctgcg ccgcggccgc gcgcggcctg cgcgtgcggc tgctggaaaa gacggaccgg
     2701 gtcggcggca ccgcggcgat ctccggcggc atggtctgga tccccggcaa ttcgagaatg
     2761 ccgggcgccg gccttgccga cagcgaggcc gcggtgcgcg cctatctcaa cgctacgctg
     2821 tcggccggta cgcgcccggc gctcattgac gcgttcgtgc gcgagggccc ggcggcgatc
     2881 gactggctcg aggcgcgcac cgaactgcgc ctgaagcccg tcacgcgcta tcccgactac
     2941 taccccgcgc ttcccggcgc caccccgggc gggcgcgtgc tcgagcccgt cggctacgac
     3001 gcgcgcgcgc tcggggccga cctcgcgctc ctgcgcccgc cgctgcccga gttcacgctg
     3061 ttcggcggca tgatggtgga cagggccgac ctgccccatt tccgcgccgt gacgcgctcg
     3121 ccgcgctcca tggcgcgcat cgcccggctg ctcgcgcggc acgcgcgcga gcgcctcgcc
     3181 gccccgcgcg gcacgagcct cgtgctcggc aacgcgctca tcgcgcggct gctgcgctcg
     3241 gctcggctcc tcggcgtcga gatccgcacg cgcgccgagg tcgcggcgct ctcgcaagag
     3301 aacgggcacg tccgcggcgt cgtgttgggc gacagcactc ggctcgaagc acgccgcggc
     3361 gtcgtgctcg cgaccggcgg cttctcgcac gatgcggagc gccgcgcgcg ctggctgccc
     3421 cggggcaccg acgtcgcctc ggccgtctgc gacggcgacg cgggcgacgg cctgcgcttg
     3481 ggcgaatccg ccggcggccg ggtggccgaa ggcaatctgc acaacgccta ctgggcgccg
     3541 atctcgaagt tccggcgggc cgacggctcg gccgcggtgt tcccgcacac ggtggccgat
     3601 cgcggcaagc cgggcatcat cgccgtcgac gcccaaggcg agcgctttac cagcgaagcc
     3661 gtgtcgtacc acgagttcgc gctggccatg ttccgcgccc gcgcgattcc ggcgttcctg
     3721 ctctgcgacc ggcgcagcct ctggcaatac gggctcggcg cggtgcggcc cttcacgctg
     3781 cggctcgcgc gcttgctcga gcagaactat ctcgcctcgg gcgcgacgcc gggagcgctc
     3841 gccgatcgcc tcggcgtcga tcgcgcgggg ctcgatcgca cggtcgcgca atacaacgcc
     3901 gatgccgtcc ggggcgtcga ttcgcgtttc aacctcgggg gcgacgtcta ccagcgcttc
     3961 ctcggcgacg ccgcgcacgg accgaacccc tgcatgcggc cgatcgaggt gcccccgttc
     4021 tttgccgtgc gcctgtaccc gagcgacctc ggcaccgcgg cgggcctgcg caccgacgaa
     4081 aacgcgcgcg tgctcgacgg cgacggccgg ccgattgccg gcctgtacgc ctgcggcaac
     4141 gacatgaact cgatcatggg cggcgcctac ccggggccgg gcatcacgct cgggccggca
     4201 ctcgtgttcg gctggctcgc ggcgcgtcat ctggcggatt gcgcctagat caggccaccc
     4261 acggcttgcc gaaggccggt tccttgtaag gggtgggtgg cagctcccac gtgccggtcg
     4321 agggcgggcg cacctccgcg tccacgtgca cgtcgagcac gcaggggcgc ttgttcctga
     4381 ttgcctgctg cagcgcgccc gagaggtcct cgctcttcgt gatggtgacg ccgtcggcgc
     4441 cgcaggcgcg cgcccaggcg gcgaagtcgg ggttgtactt cttgccctgc gtctgcccgt
     4501 agtgcccgcc cgggtagaac gcggtgccga gctcgcgccc gccgaacatg ccgacctgga
     4561 tgtcgcggat cgccgcccag ctgaagttgt tccacaccag ccagatgcag ggcagcccgt
     4621 actccaccgc cgtggcgacg acgtagggcg tcatcgagaa gccgccgtcg ccgcataccg
     4681 agatgcaggg ccgctcgggc gccgcgagct gcgcgcccat tacgctgcac acgccgaagc
     4741 ccatcgagga gtagccccag ctgttcagca tcgactgcgc gcgcttcggg cgccaaaact
     4801 gcatgaacca gttgtggtgc acgcccgagt cgcaggtgag gatggcgtcg tcgggcagga
     4861 ccttctgcag cgcgtcgacg acgtattcgg gcctgagcgg cgtggtgagc ttgccgaagt
     4921 tcggccgcac gtacgcctcc cactccttgc gccagccttc cacctcggcg cgccactccg
     4981 aaagctgctt cttcagcggc ggcgcgccgc ggctctcgcg cagcccgagc gcctggagga
     5041 gctgcgcggt gaacaccttg gcgtcggcga tgatgccgag cgtcggcggg tagttgcggc
     5101 cgagctcctg cgggtcgagg tcgacgtgga tgagcttcgt cttcgggaag ttccacgagt
     5161 agcccggatg ccacgaggag gaggagcggt cgtcgaaccg cgtgccgatg gtgacgacga
     5221 ggtcggcgtg gcggcccgcc tggttcgcgg gataggcgcc gttgcggccg atgaagccga
     5281 gcgtgagcgg gtcgtcggcc ggcacgcagc ccatgccgtt cggcgaggtg atcaccggga
     5341 tgccgtaggt ctgctgcagc tgggtgatct cgggcccggc ctcggcgagc gtcgcgccgt
     5401 ggccgatgaa gagcaccggg cgcgtggcgt cggtgagcag gttcaccgct cttgcgatat
     5461 ccgcgtcgga ggcgcccggc cggtgctggc cgaacacgtg cgcggcgggc ggcagctcga
     5521 cctcgtcctc ctcctggtag acgttgtagg gcacgtcgat gttcaccggg cccggccgcc
     5581 cggtcaccat ggtgtcgaac ccctggcgca gcgcgagcgg cagcatgtcc acgcgcgtgg
     5641 gctggaacgc gcgcttcacc accgggcgga tcaccgaggg gaaatccgcc tggttgtgct
     5701 tgtagatctc ctggaacggc gcgcggttca tctgcgaggt gggcacgttc gacgtgatgg
     5761 cgaggaacgc cgaagaatcc gagagcgcgg tggcgagcga catgaccagg ttcgccgagc
     5821 ccgggccggt cgaggtgagc gtggccaccg gctggtgctt tacgcggaag tagccatcgg
     5881 ccatgtggcc cgcgcactgc tcgtggcgcg gcgaaacgag cttcagctta tcgcgcaccg
     5941 cgtacagcgg gtcgaggatg ccgacgttgc cgtgcccgca gatgccgaag atatacggca
     6001 ccttctgctg gatcaggtat tcggcgatca gctcgccgcc tttcattcgc gccatcgcct
     6061 agaccgccgc ttcgtccgac gcgggtgtcg tcactacaaa gaacacgagc ccgccggtgc
     6121 ctgtgttggt gatggcgtgg cgcacgccgg gcggcaggta cacgtagtcg tgcggccgca
     6181 tcaggtgctc ttcgccgtcg acggtcagca cgccctcccc ctcgatcacg tagtagatct
     6241 gctcctgcac gcggtgcgag tgctcctcga cgtaggcatt gggggcatag cgcgagatgc
     6301 ggaaatccac gcgagcgctg ccgcccttct ccggcccgac cagcatcttg gaaagcgcgc
     6361 cgccgaaatg ccccgggaac tcccgccagg cgacgcgtgc catctgctcc acgaatgcgg
     6421 cggtgccgcg cacttgtttg cggttcatag tgcccgagta taagcgcgca ctagaactgg
     6481 tacttgcgca acccgccgtc caccgggatc accgcgcccg tgatgtagcg cgcgcgcggc
     6541 gaggcgagaa agcacaccag gttcgcgatg tcggcgggct ggccgtactc gcgcaccggg
     6601 atctcgtgct cggcctgcca cttgcggaac tcgggggtgt agttgcgctc gatctgctcg
     6661 ctcatgatgc gccccggcgg gatgcagttc acggtgatgc cgtgcttgcc gacctcgatc
     6721 gacaggccct tggcccaggc gtgcatcgcc gccttggcgg caaaggcgcc gttcaggccc
     6781 tcgggctcgc tttttcccgt gatgttgacg acgcggcccc acctgcgcgc catcatctgc
     6841 ccgaggagcg cgtgcgcgag ctggcgctgg cgcgtgaagt tgagcgtcat cgcctcctcc
     6901 cactgtgcct cgctcgaatc gagcttgaag gcgcgcgagc cgccggcgtt gttgacgagg
     6961 atgtccaccg cgcccaggcc gttcagcgcc gcgtctgcaa tcttctgcgg cgcgccctcg
     7021 gccatgaagt cctgctcgat gatcaccagc ttcgcgccgg tttccttctc cagcgcctcg
     7081 agtcgctcgc gccggcgcgc cgtgaccgcg aggcgcacac cctcggccgc gagcgcgagc
     7141 gcgatgccgc gcccgatgcc ggcggaagcg ccggtgacca gcgcagtctt gccactcaat
     7201 tccagctcca tggcgcctca gaattgttgc gtgtactgcg ccgggtcgat gtccgccccg
     7261 acgaccagcg gccggtcctt gggccgcttt tgcgcgagca ggcgctccag cgcctgcgca
     7321 ctgtccaccc gggcgccctc gcagcgcatc gcgcgcgcga gctgcacgat gtcggtcgcc
     7381 tcgatcagcg tgccccagct cgggtacttg cggttcgcct gcttgatctc gatgcggttg
     7441 aggctgccgt cgcagaacac gaccacgagc agcggcagct tcaggctcgc cgcgagcctg
     7501 agctcgccct gcaccatggc gagcccgccg tcgccgacga agcacaccac cggctgcttc
     7561 gggtgcagca gttgcgcgac gatcgccgcg ggcagcgagt agcccatcga ggaaaggccg
     7621 ttcgacatga gcacgccccg cggcgcagtg gtggtccagc cctgcccgac gagcagcttg
     7681 tgcgagccga cgtcggtggt ggcgatcgcc gcgcgcggca gcgccgcgcg taccgcatcg
     7741 atcacgtcgg tcggattgag ctttccctcc acgcggcctg cgtagtaggc ggcgcgcagg
     7801 gcatcgcggt ggcgcttgac cgccgcggct ttccatctcg cctcgccctt ccaggcgcgg
     7861 gcgatcgccc cgacgatcgc cgcgatgtcg cccaccagct cggtgtcggc gctgtagatc
     7921 tggtcggtgt tcggcaccgc gtcgatgtgc accgccggca ccgagagtgt ccagggcttg
     7981 atcagctcca ccgcgtcgaa gccgatggcg acgagcaggt cgcagcccct gaggaactcc
     8041 cacatcagcg cgttgcacgc catgtcgagc gtgccggcgt agtacgcatg gtcctcggcg
     8101 agcacgccct tcgccatcgg cgccaccacc accgggatgc ccgccttctc ggcgagcccg
     8161 gcgagggcgg cgtgcgcatt cgcgcgctgc gcggcgatgc cggcgaggat caccggcttt
     8221 ttcgccttcg cgagccgccg ggcgacgccg ccgtcgccgc cgaacgcggt cacggcaagc
     8281 ggcggcagcg ccaccttgtc gtcttgcgcc tcggcgccga ccacgtcggc gtgcgtggtg
     8341 atgtgcaccg gccccggacg ctcggccgtc gccgtgcgta gcgcgcggcg catgacgccc
     8401 gccaccgtgt cgggcgcgat ctcggtcgtc cacttgctca ccggcgaaaa gagccggttg
     8461 tggtcgagca cctggtgggt gaaggtctgc gcgcgcttgc gctcgatctg cccggagagc
     8521 gcgatcatcg gcacgcggtc gaggtacgcg ttggccaccg cgttgacgag gttggaagag
     8581 cccgggccga gcgtcgacat gcacacgccg ggccggccgg tgagaaagcc gtaggcctcg
     8641 gccatcaggc ccgcggtgcc ctcgcgccgg ccgagcacga actcgagccc tgcgcggcgc
     8701 gcggcttcca tgaactcgat gttgggatcg cccggatagc cgaagacgtg gccgatgccc
     8761 gccgccttca ggtaccgcac catgacctcg gagacgttca tcgctccgga ttgtgccaaa
     8821 ctgcgcgcac ttgaggagga cgggttcatg ccgcggggcg tggtcgatct ggaaggactg
     8881 aagagcccgc cggccgaggc gctgcggcgc atggcgccgc ctgccgggct cccgttccag
     8941 gtgcgcaagc tcggccacgt ggtgctcaag gtgaaggacg tgaagcgctc ggccgccttc
     9001 tataccgggg tgctgggctt tcgcgtctcc gacgtctacc ccgagagcat gaagaagggc
     9061 ggcacggtgt tcctgcgctg caacgccgac caccactgcc tcgagatgtt ctgggggctc
     9121 gaccaggtcg gcagcgatga ccgctcgcgc ccgcccggcc cggacacgac gctcgcctcg
     9181 aaatgagcga gtcgatcgcg ttcatcggct tcggcgaggc cgggcagacg atcagccgcg
     9241 ggctggcgaa ggaagcggcg cagcccgcgc tccgcgccta cgacatcctt ttcggcgcgc
     9301 cggccggcgc gaagctcgaa gcggccgcga tggcgctcgg cgtcggcctg gcgcgcgacc
     9361 acgtggaggc cgtgcgcgag gcaggcttgg tgatcctcgt cgtcaccgcg tcctcgagcc
     9421 tcgaggcgac gaagtcctgc ctgcccggcc tgcgcaaggg acagctgttc ctcgacatga
     9481 actcggtctc gcccaagcgc aagctcgaga ccgccgcgct cgtcgccccg accggcgcgg
     9541 cctacgtgga ctgcgcggtg atggcgccgg cggcgcccta cctgcacaag gtgccttgcc
     9601 tcgtcggcgg tcccggcgcc gccgcgctcg cgccgcgcgc cgcggcgctc ggcatgaaga
     9661 tggaattggt ctccgacgag gtcggccagg cctccgccat caagatgttc cgctcgatcg
     9721 tggtgaaagg gctggaggcg ctgctcgtcg agtcgatgac cgcctgcgcc gagtacggcg
     9781 tcgaggatcg cgtccttgcc tcgctcaagg agacctggcc cggcatcgac tggcaaaagc
     9841 tctcgggcta catgatcgag cgcgtggtca cgcacggcag gcgccgcgcg gccgagatgc
     9901 gcgaggtgtc ggaaacgctg cagagcatcg gcatcgaccc gctgatggcc gccgccaccg
     9961 ccgagcgcca gcagtgggtc gccgacctgg gcgtgaaggc gtcctcgcag gatcgcgccg
    10021 agctggtggg cgtcatccgc aaggcgatgg gcaagtgaag gcgctgctcg gcgagtatcc
    10081 ctgcacggcg ctgttcaggc agcgcgcgcc gctcgcgttc gaggacgtga aaccgccgag
    10141 cgccggcttc aagcgcgtgg tgcgcaacct cgaattcgat ttcgccgagc tcgccatcac
    10201 caccttcctg atggcgaaag cggcgggcaa gccctaccgc ctgctgcccg cggtggtgct
    10261 cgcgcgcatg cagcacccgc gcctggtctg cgaccgcacg cgtccggtcg cgccgcgcac
    10321 gctcgaaggc aggcgcgtgg ccgtgcgctc ctactcggtg accaccggga tgtggatccg
    10381 cggcatcctc gccgaggacc acggcgtgga ccaccgccgc atcacctggg tcacgttcga
    10441 ggaggcgcac gtcgcggagt tccgcgatcc gccgaacgtc gagcgcgcgc cggcaggcag
    10501 gaaaccggag gagatgctgc tcgcgggcga cgtggacgcc gccgtcctgg gcgatgcgcc
    10561 cgtcaccgac ccgcgcatcg tgccggtgat cccggatgca gaggccgcgg cgcgcgaatg
    10621 gcgcgcgcgc aagggcgcga tccagatcaa tcacatggtt gctatcaagc cgcacgtcga
    10681 cgcggcgccg ctcatgcgcc tgctcgagga gagctttgcc gccgccggca acccggagat
    10741 gaacccgatc gggctggacg cgaaccggcg caacctcgag gtggcggtgg acttcgtgca
    10801 ccggcagggc ctcgtcccgc aacgctacgc ggtggaggaa ctgctcgcgt gatcatcgac
    10861 tgccacggcc attacacgac cgcgccgcag gcgctgtggg actggcgcag caagcaggtc
    10921 gccggccagg acccgggccc cttgcggatt tcggacgacg agatccgcga gacgctggaa
    10981 cagaaccagc tgcgcatcca gcgcgagcgc ggcaccgacc tcacgttctt ctcgccgcgc
    11041 gcgggctcca tggagcacca cgtgggcgac gccaagacga gcgaggcctg gtcgcggcag
    11101 tgcaacgacc tgatccaccg cgtgtgccgc ctgtatccgc agaacttcgc cgcggtgtgc
    11161 cagctgccgc agtcgcccgg tgtgccgccg gagaactgca tccccgagct cgagcgctgc
    11221 gtcgaggagc tgggcttcat cggctgcaac ctcaaccccg acccctcggg cggctggtgg
    11281 aaagcgccgc cgctcggcga ccgctactgg tatccgctct acgagaagct cgtggcgctc
    11341 gacgtacccg cgatggtgca cgtgagctcc tcgtgcaacc cggccttcca cttcacgggc
    11401 gcgcactaca tcaacgccga caccaccgcg ttcatgcagt tcatccagtc ggatccgttc
    11461 aaggacttcc ccacgctcag gttcgtcatc ccgcacggcg gcggcgcggt gccctaccac
    11521 tggggccgct accgcggcct cgcccaggac atgggcaagc cgcccctgac ggagctgctg
    11581 ctgcgcaacg tcttcttcga cacctgcgtc taccacctgc ccggcatcga gctgctgctg
    11641 aaggtgatcc cggtggacaa catcctcttc gcctccgaga tcgtcggcgc ggtgcgcggc
    11701 gtcgatccgc agaccggcca gtactacgac gacacgcggc gctacatcga cgccgtcggg
    11761 tcgctgagcg cggccgaccg cgagaagatc tactcggcga acgtcaggcg cgtgtacccg
    11821 aggtttgcgc ggcgcgcctg agcggcggcc cgcgagccga tacaatcccg gctctgccat
    11881 gacaacgacc tcctcgcgct ggctctggcg gttcgtcgtg gtgctgatcc tcgccgccgg
    11941 tgtcggcggc ttcgcggcgc tcaacgccct gcgccccgag cccgcggtgc gcgcgcccac
    12001 gacgcagctg ccgctggtgc aggcggcgcc gctcgaattc cgcgacggcg ccctgcgcgt
    12061 caccggccac gggctggtga aggcgcgcgc cgaggcggtg ctgggcgtgg aggtgccggg
    12121 acgtctcgta ttcgtgagcc cgtcgctcgc cgcggggggc gcgttcacgc gcggcgaggt
    12181 gttgctgcgg ctcgacgacg agccctaccg cgcggcgctg gcgcaggccg aggccgagct
    12241 cgcctcggcg cgcgcggcgc tcgccctcgg cgagcagcag ctcgagcgca cgcgcgagct
    12301 catcgcccag ggcttccagt cgcgccaggc gctcgacgag cgctcgagca gccgcgacca
    12361 ggcggccgcg gcgttcgcgc gcgccgaggc gctcgtgcgc cagcgccgcg tggacttcga
    12421 gcgcgcggtg gtgcgcgcgc ctttcgacgg caaggtgctc tcggcgcgcg tcgtgcgggg
    12481 ggaatcggtg cagcccggca aggagcttgc gcgcttctac gccgacggcg tgctggagat
    12541 cgccgtctcg ctcaccgacc gggagatggc gctggcggcc gatccctggc gcacgggcgc
    12601 gcgcgggcgc ggcgcggcgg cgagcgtcga ggtcgagcac ggcggcggcg tgtaccgctg
    12661 gcccgcgcac gtcgaccgcg tcgagtcctc ggtggacgcc gccacgcgca ccttcaacgt
    12721 catcgtgcgc gtggacaacc cggccgagcg cggcagcgcc gtgcgcggca acgccgccgc
    12781 cgcgccgccg ctgctggtgg gcatgtacgc cacggtgcac atcgtgggca tcgaccccgg
    12841 gcgccatgcg ctggtcccgc ggcgggcgct gcgcgagggc gacgccctgt ggctgctgca
    12901 gcccggtggc gtgctgaacg tgcgcccggt gcgcgtgctg cagacctcgg gcgagcgcgt
    12961 cgcggtgtcg gccgagggcc tgcccgaagg cgcccgcgtc gtggtgagcg acctcaaggt
    13021 ggtgaccgac ggcatgcgcg tgcgcgagct cggcgccggc gagcggcgcg cttccccgtg
    13081 agggggctca ttgcctggat ggcgcgccac ccggtggccg ccaacctgct gatgctgctc
    13141 atcgtggtgg cgggcctgtc gagcgcgctc accatgaagc aggaggtgtt tcccctggtc
    13201 gagctcgacg tgatcgaggt gcgcgtcgcc tacccgggcg cggcgcccga cgaggtcgag
    13261 gaagcgatcg tgcagaagat cgaggagcag gtcgagggca tcgacggcgt cgaccgcgtg
    13321 acctcggtcg cggccgaagg cacgggcgtg gtgcgcatcg agctcgcgcg cggcgtgcag
    13381 cccaccgtca agctcgacga agtcaaggcg gcggtggacc gcatcaccac ttttcccgac
    13441 gaggccgagc gccccgaagt gtttcagctg gtcacgcgcc agcgcgtggt cgagctggcc
    13501 gtgttcggcg aggcgagcga ggcgacgctg cgcgagctcg cctaccgcat caaggacgac
    13561 ctcaccgcca cgcccggcgt gtcgctcgcg caagtggcgc gcgtgcggcc ctacgagatc
    13621 tcggtcgagg tgccgaacga cgtgctgcgc gcctacggcc tcaccctgca ggacgtcgcc
    13681 gcgacgctca agcgcgcgag cctcgacctg ccggcgggcg acatcgaggc gagcggcgag
    13741 tcgatcctgc tgcgcgcgcg cggccgcaac tacaaccgcg ccgatttcga gcgcgtgatc
    13801 gtggtctcga gcgcctcggg cgcacaggtg cgcttgggcg acatcgcgcg catcgacgac
    13861 ggcttccgcg acgacgagct gatcatgcgc ttcaacggcc gcccggcggc cttcgtgcag
    13921 gtgttccgcg tcggcgagga caaggtgctc gacgtggtgg ccaaggtgaa cgactacctc
    13981 gacacgacgc tgcgcccggg cctgcccccg gggctcgagg tgatcgtgtg gcgcgacgac
    14041 tcggccgagt tccgcaaccg ccgcgacctg ctggtgaaaa acggcgtgct ggggctcgcg
    14101 ctggtgcttg cggcgctcgc cctgttcctc gacctgcggc tcgcgctctg ggtggccgcc
    14161 ggcatcgcgg tgtccttcat cggcaccttc gccgtgatgc cgtggctcgg cctctcgatc
    14221 aacatgatgt cgcttttcgg cttcatcctc gccatcggca tcgtggtcga cgacgccatc
    14281 gtcaccggcg agaacatcta cgccgagaac gagcgcggca ccccggagat ggacgccgcg
    14341 gtgcgcggca gccagcgcgt ggtgctgccg gtggcgctcg ccgtctcgac caccatcgcg
    14401 gcgttcgtgc cgctgctgtt cgcccccggg accctgggca agttcctgtt ccagatcccg
    14461 gcggtggtga tcatcgtgct cgccgtgtcg gtgctcgagg cgatgctcat cctgccgcac
    14521 cacctcgcgc acctgcgcgt catcggctac gcgccgcgca cctggatcgg cgcgcgcctt
    14581 gccgacctgc ggcacgcggt ggaccgcgcg ttgcggcgtt tcatcgaggg ccccctggac
    14641 gcggcgctgc gcttcgccac ggcgcactac ggcgtggtca tcgcgagcgc gttctcggtg
    14701 ttcctgctca ccgtcggcgc gatcaccgcc gggcacgtgc gcttcgtgtt cttcccgcag
    14761 gtggaggggc gctacgtcac ggcgtccctc gagctcgcgc caggggcgac cgccgaaacc
    14821 acgctcgccg cggccgaaga catcgcgcgc gcgggacgcg aggcggcggt gagcctgccc
    14881 gacccgagcg gccgcgacct cgtcagcgcc gtctacatca cggtgggccg gcaggaggcg
    14941 gcaggccctg gcgcggtggg cgcgctcagc ctgctgcagg gcaacaaggc ctcggtggtg
    15001 ttcgagctcc tcgacccgga ggtgaggagc atcgcctcgg gcgacttcga gcggcgctgg
    15061 cgcgatctcg ccggccagcc cc
//
LOCUS       DBNHKE010000139        19928 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_341012, whole genome shotgun sequence.
ACCESSION   DBNHKE010000139 DBNHKE010000000
VERSION     DBNHKE010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 19928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19928
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_341012"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16O9_09275"
     CDS             complement(<1..184)
                     /locus_tag="AC16O9_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6516846.1"
                     /translation="MATETPVLELRAVDVAYHGDIRILQGLSMAVRPGKITGVIGPNG
                     AGKSTALRTMYGLLKPV"
     gene            complement(174..887)
                     /locus_tag="AC16O9_09280"
     CDS             complement(174..887)
                     /locus_tag="AC16O9_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006228029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516847.1"
                     /translation="MSAAIEVRGLTKRFGGLTALDGIELAAHPAEVLGIIGVNGAGKT
                     TLMNCICGIYRADEGRVTIGGHDVTHRTPHEIAHRGVGRTFQVPRVFRRMSLIDNLLV
                     PVLDRGETDPALIERAESTLEHMNLIELRHNFAEELSGGQQKLLEMARMLMPDPRVVM
                     LDEPFAGVHPTLCKFMIERIEAMAAGGKTVLLISHDLTSIYRLSHRVAALNEGRVIAI
                     GSVDEIRGNPAVVEAYLGN"
     gene            complement(884..1765)
                     /locus_tag="AC16O9_09285"
     CDS             complement(884..1765)
                     /locus_tag="AC16O9_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516848.1"
                     /translation="MEILVLNGIVNGLIVGGIYALIGMSLTLLYGVLRIVNFAHGEFV
                     IGGSFLAYALFSNLGLAPLYALPVAALAFFAVGWGAYYLLLPRLQKSDDPEIMSFLMM
                     YGVSIALAAVMLLIFEADSRSIDYSFTPISMKVGPIYVSTARIVAFGIVVVLAAALTW
                     FLFRTLPGKALRATIMNPEAIKIVGVDVVKLSAFAFALASAIAGVTGVLVALVFPAFN
                     PFSGAEYSIIGFIVVVLGGLGNPVGALLAGILYGLAEQLATVFLPQAMAPIIGFGILV
                     AVILIRPAGLMGWRAQR"
     gene            complement(1775..2755)
                     /locus_tag="AC16O9_09290"
     CDS             complement(1775..2755)
                     /locus_tag="AC16O9_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516849.1"
                     /translation="MPPEPSGAPRAAERASADRIYYALAAVIGGIVSVYFLFVPVLDL
                     NIAFHFYLMLWITMASAFNVAAGFSGYMPFGFVAFYGVGAFTTAILVKSLGFPVLLAL
                     PFSGLAGVILGLLFAPTLRLSGIYFAIVSLALAGICRLVISNMPERITGGSFGLQLGS
                     RAEPVHSFYVMMAVMLLALGVILWLSRSRLGKALRAVRDDAEAADMMGVNVTRVRLYG
                     WLIAAFFPAVCGGVEAWYTNVVDTETAFNTLTTAKTVIYAVAGGLGTVTGPIVGSVVM
                     VWLDELIWRQFPLLNLLILGVATVLLVLFLPRGIVGTLLRRRPEWRRYIP"
     gene            complement(2745..4148)
                     /locus_tag="AC16O9_09295"
     CDS             complement(2745..4148)
                     /locus_tag="AC16O9_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6516850.1"
                     /translation="MELTDLTLAEQAELLRRGNSSSREIVDAHLARIERLNGKLHAFV
                     EVYADEARALADAADRARKARLPLGPLHGLPIGLKDLCDIAGRVGTIGSKMWAKRVAE
                     TTCATVERLQGAGMIPLGKTHMVEFAFGGWGTNPLMGTPWNPWDLKRQRVPGGSSSGT
                     GVAVAAGLAPIGIGSDTGGSVRIPAGFNGLVGLKVTFGRISLHGTGLLSWTLDSIGPL
                     GRSVEDCMEILRVLAGPDERDPTTLGQPPLDLPEHLEAARLDRFRIALPDAKQLPPFM
                     HPAVLAAWLDAAKRLEKLGAKVVPARLPEWAFELSKPGGTIIASEAYALHRAYIEDEK
                     QPIGSAVRERVRGAAKLAPGEYAETLRVMAMRRRVFAEWFRPYDAILLPTLALPAPPL
                     DEIEEQAPVPGHLTRPWNYLGLCGLALPAGLHEGLPMSVQILGKPFAERGILEIGKAF
                     QEDTGYHKLRPKLAHAA"
     gene            4259..4765
                     /locus_tag="AC16O9_09300"
                     /pseudo
     CDS             4259..4765
                     /locus_tag="AC16O9_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            5163..5714
                     /locus_tag="AC16O9_09305"
     CDS             5163..5714
                     /locus_tag="AC16O9_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6516851.1"
                     /translation="MKDGALQVYSSDHAPYAMDAKGKLPKGEATTFKEMANGVPGLEL
                     RLPLLFTFGVQAGRITLEEFVQLTATRHAEIYGLAPWKGAIAVGADADLAIWDPEKKV
                     RITKALVHDRTGYTPYEGRELRGWPVTVLSRGEVIVDEGKLNAERGRGRFLARAPSAA
                     LEPLGRQVPEMAQLAAWNTPLQL"
     gene            5723..6682
                     /locus_tag="AC16O9_09310"
     CDS             5723..6682
                     /locus_tag="AC16O9_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HIR6516852.1"
                     /translation="MPRHLKIAAAQLGPLHRKDSRADATKRLVALLREAHGMGAKFVV
                     FPELAFTTFFPRWWMEDQAEVDAKYFERTLPSAETQPLFDEANKLGIGFYIGYAELAE
                     EKGKLRRFNTAVLVGPDGAIVGKYRKVHLPGHADHKPKAAFQHLEKKYFEVGDLGFRV
                     WRFMDTITGMLICNDRRWPEAFRVLALQGAEIVALGFNTPTENLHYPEPPALRVHHHL
                     IMAQAMAFQNATWLVETAKCGYEDGYRMFGHSLIVAPSGEIAVKSQTEEDEVICYNCD
                     IDLAANLKRTMFNFAAHRRPEHYRLIVERVGAEVTPANEAPQR"
     gene            6679..7290
                     /locus_tag="AC16O9_09315"
     CDS             6679..7290
                     /locus_tag="AC16O9_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6516853.1"
                     /translation="MTDLAIAELTPLERYKLLIGLVIPRPIAWISTVNEKGVANCAPF
                     SFFNVFSEEPPLCMVSFNRRTDGKLKHSLDNIRKTGEFVVNLVDEATANAMHLSSYEY
                     DEAESEFEKTGLTPAKAKLVRHPRIAEAAACFECKVERRLEWGTGREMVIGEILLVHA
                     REGVIDPVSKRVSEEHYKPIGRLFANRYCTTRQRFNLPGKLPE"
     gene            complement(7294..7962)
                     /locus_tag="AC16O9_09320"
     CDS             complement(7294..7962)
                     /locus_tag="AC16O9_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR6516854.1"
                     /translation="MAQRILVINPNSTEAVTRGIDEACEPLRLKDGPGIDAVTLKEGP
                     PGVETQQHVDGVVAPLINLVRASETGYDAFVIACYSDPGLHSLREVTKKPVLGISECG
                     ILTALTLGQKFGVIAILKQSIARHLRYVGALGVSERMAGELPVGLPVTELADEKKTFG
                     RMVAVGQALRDTHGADVVVMGCAGMARYRKPLQDAIGIPVVEPTQAAVAMALARVRLN
                     WAGL"
     gene            complement(7965..8636)
                     /locus_tag="AC16O9_09325"
     CDS             complement(7965..8636)
                     /locus_tag="AC16O9_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR6516855.1"
                     /translation="MDRLVHSFFNLDILAQAWPLLLAGFWMTVKLTLVVVPLGLAGGL
                     VVALASTSRSRIVRGLTIIYVDFFRAFPPLVLLMFIAFGLPFLGFDPGAFVAVALSFL
                     LNTSSYYGEIFRAGIESITRGQHEAARSTGLTGPQTMAYVVLPQAVKNVVPDLVSNTL
                     EVIKLTSLASVVALPELLFAARQAQSLTYNATPIIAAGIIYLVCLWPLVRLLSRFEHR
                     QIALH"
     gene            complement(8641..9318)
                     /locus_tag="AC16O9_09330"
     CDS             complement(8641..9318)
                     /locus_tag="AC16O9_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR6516856.1"
                     /translation="MQLERLADTFFNLQVMQQYLPKIVEGFFVTIQIALAVVLAGIAA
                     GLALALVRAFRIKAVNFCIILGVDVLRALPPLVLIIGFYFALPTIGIRMSAWVATWLA
                     LSLVLAAFAEEIFWAGILAVPKGQWEAARSTGLTFVQTLTDVVMPQAVRLTIPPLTNR
                     TIAITKGTALGAVIAVGEILYQAQTAYSFSYNPSPLVLGAAAYLILFIPVVYFGRWVE
                     TRFAWKR"
     gene            complement(9322..10065)
                     /locus_tag="AC16O9_09335"
     CDS             complement(9322..10065)
                     /locus_tag="AC16O9_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR6516857.1"
                     /translation="MSGEAPILEVRGLHKSFGALEVLKGIDLAVAKGELVFVIGPSGS
                     GKSTLLRCCNRLEEPTSGSIRVEGVDVMAPGTNINAVRRRIGMVFQSFNLYPHLDALG
                     NVALALRKVVGKPRAEAERLGRAALERVGLAEKAASFPNELSGGQQQRVAIARALALE
                     PAILLFDEPTSALDPELVGSVLAVMRELRESGMTMVVVSHEMRFARSAADRVIFMDHG
                     VIVEQGPPQKLFNAPEHPRIRDFIASIEH"
     gene            complement(10070..10906)
                     /locus_tag="AC16O9_09340"
     CDS             complement(10070..10906)
                     /locus_tag="AC16O9_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6516858.1"
                     /translation="MKRIAIMLVAAAFAGAALAQDKPLIAGVDGTFAPHAMPKLGGGV
                     EGFNVDLINEIAKQMGKKIEIFAGQFSGLIPAMNAGQISFVGAPTTVTPERAQNLLFS
                     EGYLNTFYAFVVPANAADVKALEDLKGKTISLNRGSAYDKWAQENAAKYGFKVESFGT
                     NADSVQALISGRADVQMSGNTVAAYSVLKANGQTKLATLKVDQGLVWGAAFRKDDPAL
                     RNAVDEAIECLKMNGTLPRLYEKWFGHKAPPGSATVTMYPGYGVPGMAGHDPKEHTPS
                     CK"
     gene            complement(10989..12443)
                     /locus_tag="AC16O9_09345"
     CDS             complement(10989..12443)
                     /locus_tag="AC16O9_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HIR6516859.1"
                     /translation="MNDKRGNAMKFQTQLLIDGQFVDGEGRAEEILNPATGTALASVR
                     EASFDQLNRAVGAAHRAFASWSETTPAERSALLLKLAAGIEERAEEFARIESLNCGKP
                     YARVLADEMPAIVDCFRFFAGAARCMPGSAVNEYLAGHTSMIRRDPIGVVGSVAPWNY
                     PLMMAAWKAAPALAAGNTVVLKPSEQTPLTSLRLAQLAAEIFPKGVLNIVCGRGDSVG
                     APLIEHPLVRMVSLTGDVATGQKVLQAAAKSLKRTHLELGGKAPVLVFDDADLAEVVE
                     GIRTFGYYNAGQDCTAACRIYAGSKVYERFAADLSSAVSSIKVGTQDEDGVEIGSLIS
                     ERQRNRVASFVERAATAGHMEVLTGGKLRAGKGFFYEPTVIAGAKQEDEIVRREVFGP
                     VVSVTRFTEEAQAIEFANDSDYGLASSVWTTDIRKAMRVAKRLQYGCTWINTHFMLVN
                     EMPHGGMKASGYGKDMSMYALEDYSVVRHVMAKI"
     gene            complement(12440..13255)
                     /locus_tag="AC16O9_09350"
     CDS             complement(12440..13255)
                     /locus_tag="AC16O9_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516860.1"
                     /translation="MRSERRGGASRTLIAGALAGMAFLHVPMLFIFLYAFSSEDQTYL
                     FPPPGLTTKWFGVAWERPDVWQAIRLSLEVATVTTALALVLGTLAALALARTRFFGRE
                     AVSLLLVLPIALPGIVTGIALRSAYGLSGIPFGFWTIVVGHATFCIVVVYNNAVARLR
                     RTSPSLVEASMDLGADAFQTLRYVLLPSLGTALLAGGMLAFALSFDEVIVTTFTAGQQ
                     TTLPIWMLQELVRPRQRPVTNVVAVVIVALTFIPILAAYYLTRGEEHASGGRL"
     gene            complement(13245..14159)
                     /locus_tag="AC16O9_09355"
     CDS             complement(13245..14159)
                     /locus_tag="AC16O9_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6516861.1"
                     /translation="MSAIARNVSTALYGRRRLALTLLLGLPLFWLGVVYCGSLLALLA
                     NSFFGMDPFSGMVKREFTLQNFVDLGAPANLDVIVRTVAMAAAVTLGCAAIGFPIAYL
                     MARYTRGWVRAAFYLGVMLPLWSSYLVRVYAWKLLLAKEGVVNWVFAESGLGWMLNAL
                     LAIPVIGGPSLSISTLGTFIVFVYIWLPFMILPIQAALERVPQSLIEASADLGARDAA
                     TFRKVIFPLALPGIVAGSIFTFSLTLGDYIIPGIIGDSSLFLGQVVFLQQGTAGNVPL
                     AAAFTMVPIAVMAVYLAIAKRMGAFDAL"
     gene            complement(14156..15151)
                     /locus_tag="AC16O9_09360"
     CDS             complement(14156..15151)
                     /locus_tag="AC16O9_09360"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516862.1"
                     /translation="MVAAVAFDDVSCAFGPVLAVDRVSLEVREGEFFTLLGPSGSGKT
                     TCLRMIGGFQFPTRGRVLIHGADATRRPPYERDVNTVFQDYALFPHMNVRDNIAYGLM
                     VRGVGKAERYRRADEMLELVHLPGSGARRPAELSGGQRQRVALARALINRPRVLLLDE
                     PLGALDLKLRESMQVELKALQRQVGITFVFVTHDQQEALSMSDRVCVFDRGRVEQTGT
                     PEEIYDRPATRFVAGFVGTGNLLEGAQAQRLAGVDSAVMLRAERIAIEPRRAGGVPGV
                     VKEVEYLGAFIRYIVDAGDGVELLVNAARREFERGAEVTLTWPEAACHRVPEARA"
     gene            complement(15210..16376)
                     /locus_tag="AC16O9_09365"
     CDS             complement(15210..16376)
                     /locus_tag="AC16O9_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516863.1"
                     /translation="MNIPRSTLGGALALAAALSMTAAEAAGPKKAIGKLEGRVDIVAW
                     PGYIERGATDKNFDWVTGFEKATGCKVQIKTANTSDEMVALMNEGGFDLVTASGDASL
                     RLIAGGRVQEINTALVPSYGKIDERLQKAPWHTVNGKHYGVPYQWGYNVLMYNTNVFK
                     QAPDSWAVVFEEMNLPDGKPNKGRVQAFDGPIYIADAALYLMTKNPGLGIKDPYELNE
                     DQYKAVLGLLRQQRKLVARYWHDAFVQIDDFTNEGFVASSAWQFQANILKSKNRPVAT
                     SVPKEGVTGWADTTMMHANAKHPNCAYKWLEHSINPKLQGDLAAWFGSVPVRLEACKG
                     NPLLTDQGCERHGLKAFDKIHFWRTPVTNCKTQGGKCVPYYRWVSDYIAVLGGR"
     gene            complement(16496..17428)
                     /locus_tag="AC16O9_09370"
     CDS             complement(16496..17428)
                     /locus_tag="AC16O9_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6516864.1"
                     /translation="MNLTLRQIRYFIAVAEAASLSSAARGLAISQSALTTAVRDLERG
                     LGVRLLERSSSGVTLTRDGHLFLTHAKRVLAAVAEANHALHGKPSTRRGQLTIGVTSL
                     VAGYYLAELLDRYRRAYPDVEIAVAEDEHGFLEHMLINGEVDVAILMLNTLREQRALG
                     AEVLTRSPNRLWLGADHPLCAAAEVSLRDVAALPQITLVADGIDEVMAGIWRRSGLAA
                     PTVLRTGSLEAVRSLVATGVGVAVLPDFAYRPWSLEWDRVESRSVRDELPTVDIGLVW
                     RRGTELNWTAHDFIEVARDQSRAKSRQTRSGSRG"
     gene            complement(17467..18075)
                     /locus_tag="AC16O9_09375"
     CDS             complement(17467..18075)
                     /locus_tag="AC16O9_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6516865.1"
                     /translation="MKTLRIFAAVLLAAVALPAALAQEDEAVFLVAHPMFRDLDYRQS
                     VLIAAPAPNGGHIGVIINRPTRRSLGSLFPEHEPSKKVVDPVYYGGPFSRGALVALVK
                     AEATPGSGSVQLMKNLYLAFRANTIDQVIEATPNKARYYVGYVGWRPGELRREIDRGL
                     WSVLDADLEVVFRKDTEGMWEELLQQTRRIRAGVVPAVLATR"
     gene            complement(18098..18646)
                     /locus_tag="AC16O9_09380"
     CDS             complement(18098..18646)
                     /locus_tag="AC16O9_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6516866.1"
                     /translation="MSARLRSAALALLLAAAGAAAQQHEPANGVLLVAKPELEDPNFG
                     RSVVLVTQTRDGKTLGVILNRPTPARHEGRPLWFGGPVMIRSVVALFEAPQPPEAAAF
                     HVLKNVYLSMHPQNLAALLAAPGPRFRLYAGFAAWAPGQLEREFGRDAWLVLPADETL
                     LFREDMSGLWEELLQKAAGPKT"
     gene            complement(18643..19128)
                     /locus_tag="AC16O9_09385"
     CDS             complement(18643..19128)
                     /locus_tag="AC16O9_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6516867.1"
                     /translation="MLRIFRPTVYAQLDPERLEVRDVRSGRRYAARPVGAVLDGPIPK
                     VVRIGDDALTASAERPLRIVNPFKHPRMLVAELTLGSQVLRRFLRKLYPAVLVPRQPV
                     LVLHPRIDPEGGFTEIEIRALHELGRGAGASRVLVWHGRELLPRELLSLQLTSGGKIL
                     E"
     gene            complement(19154..>19928)
                     /locus_tag="AC16O9_09390"
     CDS             complement(19154..>19928)
                     /locus_tag="AC16O9_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516868.1"
                     /translation="PEAQDTPSADAWRLFRSAQAAAARRGAADGAAARTLYGEALEHD
                     PNFARAYAASALSHVRDYRYGFARDGAAAMQRAQALATTARQIDPELPQARFALAFVY
                     LHRREHAAALAQLDEALRLDPAYGDAYGLKAHIRLDQGRIDEALELVRTAMRLSARST
                     QIHFLLLGRAYYLADDTEQALINLREAKARNTGDLEARVYLAATFARLGRSADAEWEV
                     EEIRALHPAFRAGSWLHRYPIVEPGQRRRLRADLARLGL"
BASE COUNT         3189 a         6808 c         7031 g         2900 t
ORIGIN      
        1 ccaccggttt gagcagcccg tacatcgtgc gcagcgccgt ggacttcccg gcgccgttcg
       61 ggccgatcac gccggtgatc ttcccgggcc gcaccgccat cgacaggccc tgcaggatgc
      121 ggatatcgcc gtggtaggca acgtcgaccg cgcgcagctc gagcaccggc gtctcagttg
      181 ccaaggtagg cctccacgac ggccgggttg ccgcgaatct cgtccacgct gccgatggcg
      241 atcacgcggc cttcgttcag cgccgcgacg cggtgcgaga ggcgatagat cgaggtcagg
      301 tcgtggctga tcagcagcac ggtcttgccg ccggcggcca tcgcctcgat gcgctcgatc
      361 atgaacttgc acagcgtcgg gtgcacgccg gcgaagggct cgtcgagcat caccactctc
      421 gggtcgggca tcagcatgcg cgccatttcg agcagcttct gctgcccgcc ggagagctcc
      481 tcggcgaaat tgtgccgcag ctcgatcagg ttcatgtgct cgagcgtgct ttccgcgcgc
      541 tcgatcagtg ccgggtcggt ctcgccgcgg tcgagcaccg gcaccaggag gttgtcgatc
      601 agcgacatcc tgcggaacac gcgcgggacc tggaaggtgc ggccgacgcc gcggtgcgcg
      661 atctcgtgcg gcgtgcgatg cgtgacgtcg tgcccgccga tcgtcacccg gccctcgtcg
      721 gcgcggtaga tgccgcagat gcagttcatg agcgtggtct tgcccgcgcc gttgacgccg
      781 atgatgccga gcacctctgc gggatgcgcc gcgagctcga tgccgtcgag cgcggtgagg
      841 ccgccgaagc gctttgtcag cccgcggact tcgatcgccg cgctcatcgc tgggccctcc
      901 agcccatcaa gcccgccggc cggatcagga tcaccgccac caggatgccg aagccgatga
      961 tcggcgccat cgcctggggc aggaacaccg tggcgagctg ctcggcgagg ccgtagagga
     1021 tgcccgcgag cagcgcgccg accgggttgc ccaggccccc gagcaccacc acgatgaagc
     1081 cgatgatcga gtactccgcg cccgagaatg gattgaacgc ggggaagacg agcgccacca
     1141 ggacgccggt cacccccgcg atggccgagg cgagcgcaaa ggcgaaggcg gagagcttga
     1201 ccacgtcgac gccgacgatc ttgatcgcct cggggttcat gatcgtcgcg cgcagcgcct
     1261 tgccgggcag ggtgcggaag aggaaccacg tgagcgccgc ggccagcacg acgacgatgc
     1321 cgaaggcgac gatgcgcgcg gtcgagacgt agatcggccc gactttcatc gagatcggcg
     1381 tgaaggagta gtcgatcgag cgcgagtcgg cctcgaagat gagcagcatc accgcggcga
     1441 gcgcgatcga cacgccgtac atcatgagga acgacatgat ctcggggtcg tcgctctttt
     1501 gcaggcgcgg caagagcagg tagtacgcgc cccagcccac ggcgaagaac gcgagcgccg
     1561 ccaccggcag cgcgtagagc ggcgccaggc ccaggttgct gaacagcgcg taggcgagga
     1621 acgagccgcc gatcacgaac tccccgtgcg cgaagttgac gatgcgcagc acgccgtaga
     1681 gcagcgtcag gctcatgccg atcagcgcat agatgccgcc gacgatcaag ccgttgacga
     1741 tgccgttgag gaccaggatt tccacgcgcg cgccctaggg aatgtagcgg cgccactcgg
     1801 ggcgtcggcg caggagcgtg ccaacgatgc cgcgcggcag gaacagcacc agcagcaccg
     1861 tggcgacgcc gaggatgagg aggttcagga gcgggaactg gcgccagatc agctcgtcca
     1921 gccacaccat gaccaccgag ccgacgatcg gcccggtgac cgtgccgagg ccccccgcca
     1981 ccgcgtagat caccgtcttc gcggtcgtga gcgtgttgaa cgcggtctcg gtgtcgacca
     2041 cgttggtgta ccaagcctcg acgccgccgc acaccgccgg aaagaacgcc gcgatcagcc
     2101 agccgtacag gcgcacgcgc gtcacgttca cgcccatcat gtcggcggcc tcggcgtcgt
     2161 cgcgcaccgc gcgcagcgcc ttgcccaggc gcgagcgcga cagccacagg atcacgccga
     2221 gcgcgagcag catcaccgcc atcatcacgt agaaggagtg caccggctcg gcgcgcgagc
     2281 ccagctgcag gccgaacgag ccgccggtga tgcgctcggg catgttggag atgacgaggc
     2341 ggcagatgcc ggcgagcgcg aggctgacga tggcgaagta gatgccggac aggcgcagcg
     2401 tcggcgcgaa cagcagcccc aggatcacgc ccgcgaggcc cgagaagggc agcgccagca
     2461 gcaccgggaa gccgaggctc ttgaccagga tcgcggtggt gaaggcgccc acgccgtaga
     2521 aggcgacgaa gccgaagggc atgtagcccg agaagcccgc agcgacgttg aacgccgagg
     2581 ccatcgtgat ccacagcatg aggtagaaat ggaacgcgat gttcaggtcg agcaccggca
     2641 cgaacaggaa atagacgctc acgatgccgc cgatcaccgc ggcgagcgcg tagtagatgc
     2701 ggtcggcgga ggcgcgctcg gcggcgcgcg gcgcgccgga cggctcaggc ggcatgggcg
     2761 agcttggggc gcagcttgtg gtagccagtg tcttcctgga acgccttgcc gatctcgagg
     2821 atcccgcgct cggcgaacgg cttgccgagg atctgcacgc tcatcggcag accctcgtgc
     2881 aggccggccg gcagggcgag cccgcacagg ccgagatagt tccagggccg cgtcaggtgc
     2941 ccggggaccg gcgcctgctc ctcgatctcg tccagcggcg gcgcgggcag cgctagcgtg
     3001 ggcagcagga tcgcgtcgta cgggcggaac cactccgcga acacgcgccg gcgcatggcc
     3061 atcacgcgca gcgtctcggc gtactcgccg ggcgcgagct tggccgcgcc gcgcacgcgt
     3121 tcgcgcaccg cggagccgat cggctgcttt tcgtcctcga tgtaggcgcg gtgcagcgcg
     3181 taggcctcgg aagcgatgat cgtgccgccg ggcttggaca gctcgaacgc ccactcgggc
     3241 aggcgcgccg gcacgacttt cgcgccgagc ttctccaggc gcttcgcggc gtccagccag
     3301 gccgcgagca ccgcggggtg catgaacggg ggcagctgct tggcgtcggg cagcgcgatg
     3361 cggaaacgat ccaggcgcgc tgcctcgagg tgctcaggca ggtcgagcgg cggctggccg
     3421 agggtggtcg gatcgcgttc gtccgggccg gcgagcacgc gcaggatctc catgcagtcc
     3481 tccaccgagc gtccgagcgg gccgatgctg tccagcgtcc agctcaggag cccggtgccg
     3541 tgcaggctga tgcggccgaa ggtgactttc agcccgacga gtccgttgaa gccggccggg
     3601 atgcgcaccg agccgccagt gtcgctgccg atgccgatcg gcgcgaggcc cgcggccacc
     3661 gcgacgccgg tgccgctcga ggagccgccc ggcacgcgct ggcgcttcaa atcccagggg
     3721 ttccacggcg tgcccatcag gggattggtg ccccagccgc cgaaggcgaa ttcgaccatg
     3781 tgggtcttgc ccagtgggat catcccggcg ccttgcagcc gctccacggt ggcgcaggtc
     3841 gtttcggcga cgcgcttggc ccacatcttg gagccgatgg tgcccacgcg cccggcgatg
     3901 tcgcacaggt ccttcaggcc gatcggcagg ccgtgcagcg gcccgagcgg cagcctggcc
     3961 ttgcgcgcgc ggtcggcggc gtcggcgagc gcgcgcgctt cgtcggcgta cacctcgacg
     4021 aaggcgtgca gcttgccgtt cagtctttcg atgcgcgcga ggtgcgcgtc gacgatctcg
     4081 cggctcgagc tgttgccgcg gcgcaggagc tcggcctgct cggcgagcgt gaggtcggtg
     4141 agttcgatgg cggttctccc cctgagagag cggcatgttc gctgcggcgc gcccggactg
     4201 tcaacgcgct ttcgctgcgc gttattgacg cgccgggtgc gccgccgata atgcggccat
     4261 ggccctggac ctcgtgatcc gcggcggcaa ggtcgccacc gccggaagga cgctcgattg
     4321 cgacgtcggc atccgcgatg ggcggatcgc cgagctgggg cagggcctga agggcgagcg
     4381 cacgatcgag gcgcgcggca agctggtgct gccgggcggc gtcgacagcc attgccacgt
     4441 ggagcaggtg tcctccttcg gcatcctgtg cgcggacgac ttctactcgg cgacggtgtc
     4501 cgcggcccac ggcggcacca ccaccatcgt gccgttctgc gcgcagcacc gcggcgactc
     4561 gctcgcggcg gtgctcgccg actaccacca gcgcgcgcag gcgaaggcgg tgatcgacta
     4621 cggctttcac ctgatcatcg ccaacccgga cgcgcagacg ctcggcaccg acctgccggc
     4681 ggcgatccag acgggcatcc gctcgttcaa gatcttcatg acctacgagc gcatgcgcct
     4741 catggacgag cagatcctcg acgtgatggc cgccgcgcgg cgcgacgggg cgctcgtcat
     4801 ggtgcacgcc gagaaccacg gcatgatctc ctggctcgcc gggcgcatgg tgaagcaggg
     4861 caacacgctg ccgcgctacc acgccatctg ccacacgcgc ggctcggagt ccgaggcgat
     4921 ccagcggatc gtggcgctcg ccgagctcgt cgactgcccg atcctcatcg tgcacgtgtc
     4981 cacccccgag ggcatcgagg cgatccgcca ggcgcgcgcg cggcctcaag gtctacggcg
     5041 agacttgccc gcagtacctg tttctcaccg ccaaggacat cgacatcggt ctcgcgggcg
     5101 ccgccttctg ctgctcgccg ccgccgcgcg acgaggcggc gcaggccgcc tgctggcagg
     5161 gcctgaagga cggcgcgctg caggtgtatt cgtccgacca cgcgccctac gccatggacg
     5221 cgaagggcaa gctccccaag ggcgaggcca ccaccttcaa ggagatggcg aacggcgtgc
     5281 cgggcctgga gctgcggctg ccgctgctat tcaccttcgg cgtgcaggcc gggcgcatca
     5341 cactggaaga gttcgtgcag ctcaccgcca cgcgccacgc cgagatctac ggcctcgcgc
     5401 cgtggaaggg cgcgatcgcg gtcggcgcgg acgccgacct cgcgatctgg gaccccgaga
     5461 aaaaggtgcg catcaccaag gcgctggtgc acgaccgcac cggctacacc ccctacgaag
     5521 ggcgcgagct gcggggctgg ccggtgaccg tgctttcgcg cggcgaagtg atcgtcgatg
     5581 agggcaagct caacgccgag cgcgggcgcg ggcgcttcct cgcgcgcgcg ccgagcgccg
     5641 cgctcgagcc gctcgggcgg caggtgcccg agatggcgca gctcgccgcc tggaacaccc
     5701 ctttgcaact ctaggatccg ccatgccaag acacctcaag atcgccgcgg cccagctggg
     5761 gccgctgcac cgcaaggaca gccgcgcgga cgccacgaag cgactggtgg cgctgctgcg
     5821 cgaggcgcac ggcatgggtg ccaagttcgt cgtcttcccc gagctcgcct tcaccacgtt
     5881 ctttccgcgc tggtggatgg aggaccaggc cgaggtcgac gcgaagtact tcgagcgcac
     5941 gctgccctcg gcggagacgc agccgctctt tgacgaggcg aacaagctcg gcatcggctt
     6001 ttacatcggc tatgccgagc tcgccgagga gaaggggaag ctgcgccgtt tcaacacggc
     6061 agtactcgtc ggtccggacg gcgcgattgt cggcaaatac cgcaaggtgc acctgcccgg
     6121 ccacgccgac cataagccca aggccgcctt ccagcacctg gagaagaagt acttcgaggt
     6181 cggtgacctc ggctttcgcg tctggcgctt catggacacg atcacgggca tgctgatctg
     6241 caacgaccgg cgctggcccg aggcgttccg cgtgctcgcg ctgcagggcg ccgagatcgt
     6301 ggcgctcggc ttcaacacgc cgaccgagaa cctgcactac ccggagccgc cggcgctcag
     6361 ggtgcatcac cacctgatca tggcgcaggc catggcgttc cagaacgcga cctggctcgt
     6421 cgagaccgcc aagtgcggtt acgaggacgg ctatcgcatg ttcggccact cgctcatcgt
     6481 cgccccgtcg ggcgagatcg cggtgaagag ccagaccgag gaggacgagg tcatctgcta
     6541 caactgcgac atcgacctcg ccgccaacct caagcggacg atgttcaact tcgccgcgca
     6601 ccggcggccg gagcactaca ggctgatcgt cgagcgcgtc ggcgccgagg ttacgcccgc
     6661 caacgaggcg ccgcagcgat gaccgacctc gcgatcgccg agctgacgcc cctcgagcgc
     6721 tacaagctcc tcatcggcct ggtcatccca cggccgatcg cctggatctc cacggtgaac
     6781 gagaagggcg tcgccaactg tgcgccgttt tcgttcttca acgtgttcag cgaggagccg
     6841 ccgctgtgca tggtgagctt caaccggcgc actgacggca agctaaagca ctccctcgat
     6901 aacatccgca agacgggaga gttcgtcgtc aacctcgtcg acgaggccac cgccaacgcg
     6961 atgcacctct cgagctacga gtacgacgag gcggagagcg agttcgagaa gaccggactc
     7021 acgccggcga aggcgaagct cgtgcggcat ccgcgcatcg cagaggccgc cgcctgcttc
     7081 gagtgcaagg tggagcggcg cctggaatgg ggcaccgggc gcgagatggt gatcggcgag
     7141 atcctgctgg tgcacgcgcg cgagggtgtc atcgacccgg tgtccaagcg cgtctcggag
     7201 gagcactaca agccgatcgg gcgcttgttc gccaaccgct actgcacgac acggcagcgc
     7261 ttcaacctgc cggggaagct gccggagtag cccctacaac ccggcccagt tgagccgcac
     7321 gcgcgccagc gccatcgcca ccgcggcctg cgtcggctcg accaccggga tcccgatcgc
     7381 gtcctgcagg ggcttgcgat agcgcgccat gccggcgcag cccatgacga ccacgtccgc
     7441 gccgtgggta tcgcgcaacg cctggccgac cgcgaccatg cggccgaagg tcttcttttc
     7501 gtcggcgagc tcggtcaccg gcagcccgac cggcagctcg cccgccatgc gctcggacac
     7561 gccgagcgcg ccgacgtacc tgagatgccg cgcgatcgac tgcttgagga tggcgatgac
     7621 gccgaacttc tgccccaggg tcagcgcggt aaggatgccg cattcggaga tgcccagcac
     7681 cggcttcttc gtcacctcgc ggagcgagtg cagccccggg tcgctgtagc aggcgatgac
     7741 gaaggcgtcg tagcccgtct cgctcgcgcg caccagattg atcagcggcg ccactacgcc
     7801 gtcgacgtgc tgctgcgttt ccacgcccgg cgggccctcc ttgagcgtca ccgcgtcgat
     7861 gccggggccg tccttcagcc gcagtggctc gcaggcctcg tcgatgccgc gcgtgacggc
     7921 ctcggtcgag ttcgggttga tgacgaggat gcgctgggcc atggtcagtg cagggcgatc
     7981 tggcggtgct cgaagcggct cagcagccgc accagcggcc acaggcagac caggtagatg
     8041 atacctgcgg cgatgatcgg cgtggcattg taggtgagcg actgcgcctg gcgcgcggca
     8101 aagaggagct cgggcagcgc caccacgctg gcgagcgagg tgagcttgat cacctcgagc
     8161 gtgttcgaga ccaggtcagg caccacgttc ttcaccgcct gcggcagcac cacgtaggcc
     8221 atggtctgcg ggccggtgag gccggtggag cgcgcggcct cgtgctggcc gcgcgtgatc
     8281 gattcgatgc cggcgcggaa gatttcgccg tagtagctgg aggtattcag gagaaacgac
     8341 agcgctaccg caacgaaggc gcccgggtcg aagccgagga acggcaggcc gaaggcaatg
     8401 aacatcagca gcaccagcgg cgggaaggcg cggaagaaat cgacgtagat gattgtcagc
     8461 ccgcgcacga tgcggctgcg cgaggtggag gcgagtgcca ccacgagccc gccggcgagg
     8521 ccgagcggca ccacgaccag ggtcagcttc accgtcatcc agaaccccgc gagcagcagc
     8581 ggccaggcct gcgccaggat gtcgaggttg aagaagctgt ggacgagccg gtccatggcg
     8641 ctagcgcttc caggcgaagc gcgtctcgac ccagcgtccg aagtacacca ccgggatgaa
     8701 gaggatcagg taggccgccg cgccgagcac cagcggcgac gggttgtagg agaacgagta
     8761 cgcggtctgc gcctgataga ggatttcgcc cacggcgatg acggcgccga gcgcggtgcc
     8821 cttggtgatg gcgatggtgc ggttggtgag cggcgggatg gtgaggcgca ctgcctgcgg
     8881 catcaccacg tcggtgagcg tctgcacgaa cgtgagcccc gtggagcgcg ccgcctccca
     8941 ctgccccttg ggcacggcga ggatgccggc ccagaaaatc tcctcggcga aggccgcgag
     9001 caccagcgac agcgcgagcc aggtcgctac ccaggccgac atgcggatgc cgatcgtggg
     9061 cagcgcgaag taaaagccga tgatgaggac cagcggcggc agcgcgcgca gcacgtccac
     9121 gcccaggatg atgcagaagt tgaccgcctt gatgcgaaac gcccgcacca gcgcgagcgc
     9181 gaggcccgcg gcgattcccg ccagcaccac cgcgagcgcg atctggatgg tgacgaagaa
     9241 cccttcgacg atcttcggca ggtactgctg catcacctgc aggttgaaga acgtgtcggc
     9301 gaggcgctcg agctgcattc ctcagtgctc gatgctggcg atgaagtcgc ggatgcgcgg
     9361 gtgctcgggc gcgttgaaca gcttctgcgg cgggccctgc tcgacgatga cgccgtggtc
     9421 catgaagatc acgcggtcgg cggcgctcct tgcgaagcgc atctcgtggc tcaccaccac
     9481 catggtcatg cccgactcac gcagctcgcg catcactgcg agcacgctgc ccaccagctc
     9541 ggggtcgagc gccgaggtcg gctcgtcgaa gagcaggatc gcgggctcga gcgccagcgc
     9601 gcgcgcgatc gccacgcgct gctgctggcc gccggagagc tcgttgggga agctcgccgc
     9661 cttctcggcc aggccgacgc gctcgagcgc cgcgcggccg agccgctcgg cctcggcgcg
     9721 cggcttgccg acgaccttgc gcagggcaag cgcaacgttg ccgagcgcgt cgaggtgcgg
     9781 gtagaggttg aacgactgga acaccatgcc gatgcgccgg cgcacggcgt tgatgttggt
     9841 gccgggggcc atgacgtcga cgccctcgac acggatcgag cccgaggtgg gctcctccag
     9901 ccggttgcag caccggagca gcgtgctctt gcccgagccc gatgggccga tgacgaatac
     9961 cagctcgccc ttcgcgacgg cgaggtcgat gcccttcagg acctcgaggg cgccgaagga
    10021 cttgtgcagc ccccggacct cgaggatcgg cgcctcgccg ctcaacggct tacttgcagc
    10081 tcggcgtgtg ctccttgggg tcgtgccccg ccatgcccgg cacgccgtag cccgggtaca
    10141 tggtcaccgt ggccgagccg gggggcgcct tgtggccgaa ccacttctcg tagagccggg
    10201 gcagggtgcc gttcatcttc aggcactcga ttgcctcgtc gacggcgttg cgcagcgccg
    10261 gatcgtcctt gcggaacgcc gcgccccaca ccaggccctg gtcgaccttg agcgtggcga
    10321 gcttggtctg gccgttcgcc ttcagcaccg agtaggcggc caccgtgttg cccgacatct
    10381 gcacgtcggc acggcccgag atcagcgcct gcaccgagtc ggcgttggtg ccgaacgact
    10441 ccactttgaa gccgtacttg gcggcgttct cctgcgccca cttgtcgtag gccgagccgc
    10501 ggttgagcga gatggtcttg cccttcaggt cctccagggc cttcacgtcg gcggcattcg
    10561 ccggcacgac gaaggcgtaa aaggtgttga ggtagccctc gctgaacagc aaattctgcg
    10621 cgcgctccgg agtcacggtg gtgggcgcgc cgacgaagct gatctggccg gcgttcatcg
    10681 ccgggatcag gcccgagaac tggcccgcga agatctcgat cttcttgccc atctgcttcg
    10741 cgatctcgtt gatcaggtcg acgttgaagc cctcgacccc gccgccgagc ttgggcatgg
    10801 cgtgcggcgc gaaggtgccg tcgaccccgg cgatgagcgg cttgtcctgg gcgagtgccg
    10861 cgccggcgaa cgccgcggcg acgagcatga tggcgatgcg cttcatggtg ggatcccccc
    10921 tgatcggttg accggcggat tacagcatct ttccgccggg gcggaaagac gccgcgcgaa
    10981 acgcgccgct agatcttcgc catcacgtgg cgcaccacgc tgtaatcctc cagagcatac
    11041 atcgacatgt ccttgccgta gcccgaggct ttcatgccgc cgtgcggcat ctcgttcacc
    11101 agcatgaagt gggtgttgat ccaggtgcag ccgtactgca ggcgcttggc cacccgcatc
    11161 gccttgcgga tgtcggtggt ccacaccgag gaggcgaggc cgtagtcgga gtcgttggcg
    11221 aactcgatcg cctgcgcctc ctcggtgaag cgcgtcaccg acaccaccgg gccgaacacc
    11281 tcgcgccgca cgatctcgtc ctcctgcttg gcgccggcga tcaccgtggg ctcgtagaag
    11341 aagcccttgc cggcgcgcag cttgccgccg gtgagcacct ccatgtgccc ggcggtggcg
    11401 gcgcgctcga cgaagctcgc cacgcggttg cgctgccgct cggagatgag cgagccgatc
    11461 tccacgccgt cctcgtcctg cgtgccgacc ttgatcgagg acacggcgct ggagaggtcg
    11521 gcggcgaagc gctcgtagac cttcgacccg gcgtagatgc ggcaggcggc ggtgcagtcc
    11581 tggccggcgt tgtagtagcc gaaggtgcgg atgccctcga ccacctcggc gaggtcggcg
    11641 tcgtcgaaca ccagcaccgg cgccttgccg ccgagctcga gatgcgtgcg cttcaaggac
    11701 ttggccgccg cctgcagcac cttctgcccg gtcgccacgt cgccggtgag cgagaccatg
    11761 cgcacgagcg ggtgctcgat cagcggcgcg cccaccgagt cgccgcgccc gcagacgatg
    11821 ttgagtacgc ccttcggaaa gatttcggcg gcgagctgcg cgagccgcag cgacgtgagc
    11881 ggggtctgct ccgagggctt gagcaccacg gtgttccccg cggcgagcgc gggcgccgcc
    11941 ttccacgccg ccatcatgag cggatagttc cacggcgcga ccgagccgac gactccgatc
    12001 gggtcgcgcc ggatcatgct ggtgtgccct gcgaggtact cgttcaccgc cgagcccggc
    12061 atgcagcgcg ccgcgccggc gaagaagcga aagcaatcca cgatcgccgg catctcgtcg
    12121 gcgagcacgc gcgcgtaggg cttgccgcag ttgagcgact cgatgcgcgc gaactcctcg
    12181 gcgcgctcct cgatgccggc ggcgagcttg agaagcaggg cgctgcgctc cgcgggcgtg
    12241 gtctccgacc acgaggcgaa cgcgcgatgc gccgcgccca cggcgcggtt cagctggtcg
    12301 aaggacgcct cgcgcacgct tgccagcgcc gtgccggtgg cggggttgag gatctcctcg
    12361 gcgcgcccct cgccgtccac gaactggccg tcgatcagca gctgggtctg gaacttcatg
    12421 gcgttacctc gtttgtcgtt cataggcggc ctccgctggc gtgctcttcg ccgcgcgtca
    12481 ggtaataggc ggcgaggatg ggaatgaaag tgagtgcgac gatcaccacc gcgacgacgt
    12541 tggtgaccgg gcgctggcgc gggcgcacca gctcctgcag catccagatg ggcagcgtgg
    12601 tctgctgccc ggcggtgaag gtggtgacga tgacctcgtc gaacgagagc gcgaaggcga
    12661 gcatgccgcc cgcgagcagc gcggtgccga ggctcggcag cagcacgtag cgcagcgtct
    12721 ggaaggcgtc cgcgccgagg tccatcgagg cttcgacgag cgaagggctg gtgcgccgca
    12781 gccgcgcgac cgcgttgttg tacacgacca cgatgcagaa cgtggcgtgg ccgacgacga
    12841 tggtccagaa gccgaacggg atgcccgaga ggccgtaggc cgaccgcagc gcgatgccgg
    12901 tgacgatgcc gggcagcgcg atcggcagca ccagcagcag cgacaccgcc tcgcggccga
    12961 agaagcgggt gcgcgcgagc gcgagcgccg cgagcgtgcc gagcacgagc gccagcgccg
    13021 tggtgaccgt cgccacctcg agcgagagcc ggatcgcctg ccacacgtcg ggccgctccc
    13081 aggcgacgcc gaaccatttc gtcgtcagcc ccggcggcgg gaagaggtag gtctggtcct
    13141 cgctcgagaa ggcgtacagg aagatgaaca gcatcggcac gtgcaggaac gccatgccgg
    13201 cgagcgcgcc ggcgatgagc gtgcgcgagg cgccgccgcg gcgctcagag cgcatcgaag
    13261 gcccccatgc gcttggcgat cgcgaggtac acggccatca ccgcgatcgg caccatcgtg
    13321 aaagccgccg cgagcggcac gttgcccgcg gtgccctgct gcaggaacac cacctggccg
    13381 agaaacagcg acgaatcgcc gatgatcccc gggatgatgt agtcgcccag cgtgagcgag
    13441 aaggtgaaga tcgatccggc gacgatgccg gggagggcga gggggaagat caccttgcgg
    13501 aaggtcgccg cgtcgcgcgc gccgaggtcg gccgaggcct cgatcaggct ctgcggcacg
    13561 cgctcgagcg ccgcctggat cggcaggatc atgaacggca gccagatgta gacgaagacg
    13621 atgaaggtgc cgagcgtcga gatcgagagc gagggcccgc cgatcaccgg gatcgccagc
    13681 agcgcgttca gcatccagcc gaggccgctc tcggcgaaca cccagttcac cacgccctcc
    13741 ttggcgagca gcagcttcca ggcgtacacg cgcaccaggt agctcgacca cagcggcagc
    13801 atcacgccga ggtagaacgc cgcgcgcacc cagccgcgcg tgtagcgcgc catgaggtag
    13861 gcgatgggaa agccgatcgc cgcgcagccg agcgtcacgg cggcggccat cgccacggtg
    13921 cgcacgatca cgtccaggtt cgcaggcgcg ccgaggtcga cgaagttctg cagcgtgaac
    13981 tcgcgcttca ccatgcccga gaacgggtcc atgccgaaga agctgttggc gagcagcgcg
    14041 agcagcgacc cgcagtacac cacgccgagc cagaacagcg gcagcccgag cagcagcgtg
    14101 agcgcgagcc gccggcgccc gtagagcgcg gtggagacgt tgcgggcgat cgcgctcacg
    14161 cgcgcgcctc cgggacgcgg tggcaggcgg cctcgggcca ggtgagcgtc acctcggcgc
    14221 cgcgctcgaa ctcgcgccgc gcggcgttca ccagcagctc gacgccgtcg cccgcgtcca
    14281 cgatgtagcg gatgaaggcg ccgaggtact ccacttcctt caccacgccg ggcacgccgc
    14341 ccgcgcggcg cggctcgatc gcgatgcgct cggcgcgcag cattacggcg ctatcgaccc
    14401 ccgccaggcg ctgcgcctgc gcgccttcca gcaggttgcc ggtgccgacg aagcccgcga
    14461 cgaagcgcgt cgccggccgg tcgtagatct cctcaggcgt gccggtctgc tcgacgcggc
    14521 cgcggtcgaa cacgcacacg cggtcgctca tggaaagcgc ctcctgctgg tcgtgcgtga
    14581 cgaacacgaa ggtgatgccg acctggcgct ggagcgcctt cagctccacc tgcatgctct
    14641 cccggagctt gagatccagc gcgccgagcg gctcgtcgag cagcagcacg cgtggccggt
    14701 tgatgagcgc gcgcgcgagc gccacgcgct ggcgctggcc gcccgagagc tcggcggggc
    14761 ggcgcgcgcc gctgccgggc aggtgcacga gctccagcat ctcgtcggcg cgccggtagc
    14821 gctcggcctt gcccacgccg cgcaccatca ggccgtaggc gatgttgtcg cgcacgttca
    14881 tgtgcgggaa cagcgcgtag tcctggaaca cggtgttcac gtcgcgctcg tagggcggcc
    14941 ggcgcgtggc gtccgcgccg tggatcagca cgcggccgcg cgtcgggaac tggaacccgc
    15001 cgatcatcct gaggcaggtc gtcttgcccg agcccgaagg cccgagcaag gtgaagaact
    15061 cgccctcgcg cacctccagg ctcacgcggt cgacggcaag caccggcccg aaggcgcagc
    15121 tcacgtcatc gaacgcgacg gcggcgacca tgtgctgaaa aaaagggccg cgcccgcagc
    15181 gcgtcacgcc gcgggcgcgg aggaggagcc tagcggccgc cgaggaccgc gatgtagtcg
    15241 gacacccagc ggtagtaggg cacgcacttg ccgccctgcg tcttgcagtt ggtgaccggc
    15301 gtgcgccaga agtggatctt gtcgaaggcc ttcaggccgt ggcgctcgca gccctggtcg
    15361 gtgaggagcg ggttcccttt gcaggcctcg agccgcaccg gcaccgagcc gaaccaggcc
    15421 gcgaggtcgc cctgcagctt cgggttgatc gagtgctcga gccacttgta ggcgcagttc
    15481 gggtgcttgg cgttggcgtg catcatggtg gtgtcggccc agccggtgac gccttccttg
    15541 ggcaccgagg tggccaccgg gcggttcttc gacttcagga tgttggcctg gaactgccac
    15601 gcggaagagg cgacgaagcc ctcgttggtg aagtcgtcga tctgcacgaa ggcgtcgtgc
    15661 cagtagcgcg cgacgagctt cctttgctgg cgcagcaggc cgagcaccgc cttgtactga
    15721 tcttcgttca gctcgtaggg gtccttgatc cccaggccgg gattcttggt catcaggtac
    15781 agcgcagcgt cggcgatgta gatcgggccg tcgaaggcct gcacgcggcc cttgttcggc
    15841 ttgccgtcgg gcaggttcat ttcctcgaac accacggccc aggagtcggg cgcctgcttg
    15901 aacacgttgg tgttgtacat gagcacgttg tagccccact ggtagggcac gccgtagtgc
    15961 ttgccgttca ccgtatgcca gggcgccttc tgcaggcgct cgtcgatctt gccgtagctc
    16021 ggcacgagcg cggtgttgat ctcctgcacg cgcccgccgg cgatgagccg caggctcgcg
    16081 tcgcccgagg cggtgaccag gtcgaagccg ccctcattca tcagcgccac catctcatcg
    16141 gaggtgttcg cggtcttgat ctgcaccttg cagccggtgg ccttttcgaa gcccgtcacc
    16201 cagtcgaagt tcttgtcggt cgcgccgcgc tcgatgtagc cgggccaggc gacgatgtcg
    16261 acgcggcctt cgagcttgcc gatcgccttt ttcgggccgg cggcctcggc cgccgtcatg
    16321 gacagcgcgg cagcgagcgc gagcgcgccg cccagggtgc tgcggggaat gttcatggcc
    16381 tctcctcctg gctctgatta ggtgcgggat tcgcgcgctg agcctagcaa agcccgaaca
    16441 gcgataggaa attcattctt tcgctggggt gccatgcgtt ttttcgatgg cctgctcaac
    16501 cccttgagcc ggaacgcgtt tggcgggact tggcgcggct ttggtcgcgc gccacctcga
    16561 tgaagtcgtg cgccgtccag ttgagctcgg tgccgcgccg ccacaccagg ccgatgtcca
    16621 cggtgggcag ctcatcgcgc acgctgcgcg attccacccg gtcccattcg agcgaccagg
    16681 gccggtaggc gaagtcgggc agcacggcga cgcccacgcc ggtggcgacg aggctgcgca
    16741 cggcttcgag cgagccggtg cgcagcaccg tcggcgcagc gaggccgctg cggcgccaga
    16801 tgcccgccat cacctcgtcg atgccgtcgg cgacgagcgt gatctgcggc agcgccgcca
    16861 cgtcgcgcag cgacacctcg gcggcggcgc acagcggatg gtcggcgccg agccacaggc
    16921 gattcggcga gcgcgtgagc acctcggcac cgagcgcgcg ctgctcgcgc agcgtgttca
    16981 gcatcaggat ggcgacgtcg acttcgccgt tgatcagcat gtgctcgaga aagccgtgct
    17041 cgtcctcggc caccgcgatc tcgacgtcgg gatacgcgcg ccggtagcgg tcgaggagct
    17101 cggcgaggta gtagcccgcc accaggctgg tcacgccgat cgtcagctgc ccgcggcggg
    17161 tcgagggctt gccgtgcagc gcgtggttcg cctcggccac cgccgcgagg acgcgcttgg
    17221 catgcgtgag gaacaggtgc ccgtcgcgcg tcaaggtcac gccgctcgag gagcgctcga
    17281 gcagccgcac gccgaggccg cgctccaggt cgcgcaccgc cgtggtgagc gccgactgcg
    17341 agatcgcgag gccgcgcgcc gcgctcgaca gcgacgcggc ctcggctacg gcgatgaagt
    17401 agcgaatctg ccgcagggtg aggttcatgg cgctcccccg cgcgcgcaaa ggatagagag
    17461 cggcgcctag cgcgtggcca gaacggccgg gacgacgccg gcacggatgc gccgcgtctg
    17521 ctgcagcagc tcctcccaca tgccctcggt gtccttgcgg aacaccacct cgaggtcggc
    17581 gtcgagcacg ctccacaggc cgcggtcgat ctcgcgccgc agctcgcccg ggcgccagcc
    17641 gacgtagccg acgtagtagc gcgccttgtt gggcgtggcc tcgatcacct ggtcgatggt
    17701 gttggcgcga aacgcgaggt agaggttctt catcagctgc accgagcccg agccgggggt
    17761 cgcttcggcc ttcaccagcg ccaccagcgc gccgcgcgag aacgggccgc cgtagtacac
    17821 gggatcgacc actttcttgg agggctcgtg ctcggggaac aggctcccca gcgagcgccg
    17881 cgtaggccgg ttgatgatca cgccgatgtg cccgccgttg ggcgcgggcg cggcgatgag
    17941 caccgactgc cggtagtcga ggtcgcggaa catcgggtgc gccaccagga atacggcttc
    18001 gtcttcctgc gccagcgccg ccggaagcgc gacggcggcg aggagcacgg cggcgaagat
    18061 tcgcaaggtc ttcaccaagg cattgtagcg cggttgctca ggttttcggt cccgcggcct
    18121 tttgcagcag ctcttcccac agcccgctca tgtcctcgcg aaacagcagc gtctcgtcgg
    18181 cgggcagcac gagccaggcg tcgcgcccga actcgcgctc cagctgcccc ggcgcccagg
    18241 cggcgaagcc cgcgtacagc cggaagcgcg gcccgggtgc cgcgagcagg gcggcgagat
    18301 tctgcgggtg catgctgagg tagacgttct tcagcacgtg gaacgccgcc gcctcgggcg
    18361 gctgcggcgc ctcgaacagc gcgaccacgc tgcggatcat caccgggccg ccgaaccaca
    18421 gcggccggcc ctcgtggcgc gccggcgtgg gcctgttgag gatcacgccg agcgtcttgc
    18481 cgtcgcgcgt ttgcgtgacc agcaccacgc tgcggccgaa gttcgggtct tcgagctcgg
    18541 gcttggcgac gagcagcacg ccgttcgccg gctcgtgctg ctgcgcggcc gcgcccgcgg
    18601 cggcgaggag cagggcgagc gccgcgctgc gcagccgcgc gctcattcga ggatcttccc
    18661 gcccgaggtg agctgcaggc tcaacagctc gcgcggcagc agctcgcgcc catgccacac
    18721 cagcacgcgc gaagcgcccg cgccgcgccc cagctcgtgc agcgcgcgta tctcgatctc
    18781 ggtgaagccg ccctcggggt cgatccgcgg gtgcaggacc agcaccggct ggcgcggcac
    18841 cagcaccgcg ggataaagct tgcgcaggaa ccggcgcagc acctggctgc cgagcgtcag
    18901 ctcggcaacc agcatgcgcg ggtgcttgaa tgggttcacg atgcgcagcg gccgctcggc
    18961 gctggcggtg agcgcgtcgt cgccgatgcg caccaccttc gggatcgggc cgtcgagcac
    19021 cgcgccgacc gggcgcgccg cgtagcggcg gccgctgcgc acgtcgcgca cctcgagccg
    19081 ctcggggtcg agctgcgcgt acacggtggg gcggaagatc ctgagcatgc gcctgcagcc
    19141 accaacccgc cgactatagc cccagtcggg cgaggtcggc gcggagccgc cgccgctgtc
    19201 cgggctccac gatcgggtac ctgtgcagcc atgagcccgc gcgaaacgcc ggatgcaggg
    19261 cgcggatctc ctccacttcc cactcggcgt ccgccgagcg gccgagccgc gcaaaggtcg
    19321 ccgccaggta cacgcgcgcc tcgaggtccc cggtattgcg cgctttagcc tcgcgcaggt
    19381 tgatgagcgc ctgctcggtg tcgtccgcta agtagtacgc gcgccccagt agcaggaagt
    19441 ggatctgcgt ggaccgcgcg gacaggcgca ttgccgtgcg caccagctcg agcgcctcgt
    19501 cgatcctgcc ctggtcgagg cggatgtgcg ctttcagccc gtaggcgtca ccgtaggcgg
    19561 ggtccaggcg cagcgcctcg tcgagctgcg ccagggcggc cgcgtgctcg cgccggtgca
    19621 ggtagacaaa ggcgagggca aagcgcgcct gcggcagctc gggatcgatc tggcgcgcgg
    19681 tggtcgccag cgcctgggcg cgctgcatcg cggcggcgcc gtcccgggcg aagccgtagc
    19741 ggtaatcgcg cacgtggctc agcgcgctcg cggcgtaggc gcgcgcaaag ttggggtcgt
    19801 gctcgagcgc ctcgccgtag agcgtgcgcg cggccgcgcc gtcggccgcg ccgcggcgcg
    19861 cggcggcggc ctgcgcggag cgaaacaggc gccaggcgtc cgcgctgggc gtgtcctgcg
    19921 cctccggg
//
LOCUS       DBNHKE010000140        21242 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_552665, whole genome shotgun sequence.
ACCESSION   DBNHKE010000140 DBNHKE010000000
VERSION     DBNHKE010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 21242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_552665"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="AC16O9_09395"
     CDS             <1..617
                     /locus_tag="AC16O9_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6516869.1"
                     /translation="TMAALNRHNKTSHLEKPTLLLEFHGTEASVVEQAEMVQEIAKEN
                     GGLGFQWATKPEERTKLWEARHNAYFACLQLKPGSRAISTDVCVPISRLAECIVETTR
                     DIEKASMPIPLFGHVGDGNFHLVILVDPASASDMEEAKALNKRLVKRALAMEGTCTGE
                     HGIGMGKQASLKEELGEAVDLMRDLKRTFDPENLLNPGKVVPLQ"
     gene            614..1072
                     /locus_tag="AC16O9_09400"
     CDS             614..1072
                     /locus_tag="AC16O9_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6516870.1"
                     /translation="MSAEARLKKLGIDLGPVSAPVANYVNAVRTGNLLFLSGKGPRAV
                     DGKRPQGKVGREYTAEQAYQHARTVGLDLLAVMRAELGSLDKVARVVKLLGMVNAVPE
                     FQDHPKVINGCSDLFVEVLGDAGKHARSAVGMGSLPMGIPVEIEVIVEVK"
     gene            complement(1120..1665)
                     /gene="orn"
                     /locus_tag="AC16O9_09405"
     CDS             complement(1120..1665)
                     /gene="orn"
                     /locus_tag="AC16O9_09405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR6516871.1"
                     /translation="MPADANNLIWIDMEMSGLAPDTDRILEIALLVTDAQLNIVAEGP
                     VLVVHQPDEVLAAMDSWNTGVHGKSGLVDKVKASRLDEATAEVQMLAFLAAHVPSGVS
                     PMCGNSICQDRRFLARWMPKLEAWFHYRNLDVSTLKELVRRWQPEAAKGLKKESKHEA
                     MADILDSIEELKFYRKTIMTI"
     gene            1687..2931
                     /locus_tag="AC16O9_09410"
     CDS             1687..2931
                     /locus_tag="AC16O9_09410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6516872.1"
                     /translation="MNAFSWIFLAALAAATLARLWLAQRQVAHVRAHRDAVPAEFADA
                     IPLSAHQKAADYTVAKARLGMLDVLLGAAVLLALTLGGLLQRMSELAARVFDHGGLAH
                     GVALIAAAVVLLSALELPLSAYRTFVIEARFGFNRMSLGLFLADLAKHALLGAVLGLP
                     LLALVLWLMGRMGDAWWLYVWLAWIGFNLLVLLAYPTFIAPLFNKFSPLEDPALAGRI
                     EQLLARCGFTSNGLYVMDGSRRSAHGNAYFTGFGAAKRIVFFDTLLARLAPAEVEAVL
                     AHELGHYRRHHVWKRIALLFALSLGLLWILGRLIDEPWFYAGLNAGAPGDAMGLLLFV
                     LVMPVFTFFLQPLTSLYSRRHEFEADSYAAAHASAAELVRALVKLYQDNAATLTPDPL
                     HSAFYDSHPPAARRIARLQALA"
     gene            2940..3269
                     /locus_tag="AC16O9_09415"
     CDS             2940..3269
                     /locus_tag="AC16O9_09415"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6516873.1"
                     /translation="MNELAAKKCKPCEGGVAPFSDAQARDMLKQLRGWSLEDGKLVKL
                     YPFGNYHHTMAFVNALAWISHREDHHPDLEVSYNKCRVSYSTHALNGLSENDFICAAK
                     CDALFEI"
     gene            3269..3577
                     /locus_tag="AC16O9_09420"
     CDS             3269..3577
                     /locus_tag="AC16O9_09420"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR6516874.1"
                     /translation="MRRVFSSFDLVAVHHARNMLEAEGVRAVVRNERLASAMGELPPA
                     ECQAEVWVLDEADAARAEQLLREGARVPAGPPWTCAGCGERIEPQFTQCWHCGATRRD
                     "
     gene            complement(3574..4479)
                     /locus_tag="AC16O9_09425"
     CDS             complement(3574..4479)
                     /locus_tag="AC16O9_09425"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HIR6516875.1"
                     /translation="MSAIAVIVALLLEQWRPLGERKAYFALLAGGAEWLERSFNAGEG
                     RQGTTAWLVAVLPVLAASLALYFLLLWLHPLLAFAFNIVALYFTLGFRQFSHYFTDLQ
                     LAIRTGDLERARALIGEWRGEPAQHRSREEVIRLAIEEALVASHRHVFGVLFWFVLLP
                     GPSGAILYRLAAYLRRRWGGLGAFGGFAEQVGRLLEWPAVRLTAASFAVVGDFEDTVY
                     CWRTQALAWPDPDAGVVLAAGAGALGVRLGMPLSAPEGVEVRPELGVGEAADAPFLDG
                     VVGLVWRALVVWVFLLVLVALAGAL"
     gene            complement(4476..5108)
                     /locus_tag="AC16O9_09430"
     CDS             complement(4476..5108)
                     /locus_tag="AC16O9_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HIR6516876.1"
                     /translation="MRATLNHDTPPGLDPDRLRRRFAAGEPVAMLYGDQGIDAERAAA
                     RRRASVLVPIVTRPEALSVLLTRRTTHLKNHSGQIAFPGGGAEPGDASPEATALREAE
                     EEIGLEPARVEVIGRLSDYHTRTDYRVTPVVGLVAPPFTLRLDAHEVDEAFEVPLAFL
                     LDPANHQRHQREFQGRTVPFYAIPYGDYYIWGATAGMLVNLYRFLGRGGG"
     gene            5196..5438
                     /locus_tag="AC16O9_09435"
     CDS             5196..5438
                     /locus_tag="AC16O9_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516877.1"
                     /translation="MKRTILGFVLALASASAFAFHCPKDMKKIDEAMAKNPSLSAQQM
                     ADVKKYRAEGEALHKAGNHQGSVDALAKAMKILSIQ"
     gene            complement(5511..6482)
                     /locus_tag="AC16O9_09440"
     CDS             complement(5511..6482)
                     /locus_tag="AC16O9_09440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR6516878.1"
                     /translation="MSKAVRFHKTGGPEVLQLDDVQVGEPGQGQVRIRHTAIGVNFID
                     TYQRSGLYPMQLPSAAGNEGAGVVESVGPGVDGLKKGDRVAYTGQVGSYCTERLLPAD
                     RVVKVPEGISDEQAACLMLKGMTVQYLIHSTYAVKKGETVLWHAAAGGVGLMACQWLK
                     ALGVTVIGTVGSPEKAKIAKAHGCEHVIDYTKENFVERVKEITGGKKLPVVYDSVGKT
                     TWEGSLDCLRPLGLWVIFGNASGPVPPFNTQVLAQKGSLYMTRPTLVTYIAARADLEA
                     RSASVFDAVKSGKVKIEISARYKLAEAAKCHTDLEGRKTTGSVILLP"
     gene            complement(6511..6921)
                     /locus_tag="AC16O9_09445"
     CDS             complement(6511..6921)
                     /locus_tag="AC16O9_09445"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HIR6516879.1"
                     /translation="MAESNPPQDPFEIFRRLWGPLGLPVPGMAVPTLDPKEVDKRIAE
                     LRSIESWLEVNLNMVRFAAQGLEVQRSALLAMGAGAPAGAPPSALAELAAASANQAAS
                     GMLWPWAVMQQAMAGKPPASEPPPAADDENKPKE"
     gene            complement(6930..8108)
                     /locus_tag="AC16O9_09450"
     CDS             complement(6930..8108)
                     /locus_tag="AC16O9_09450"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6516880.1"
                     /translation="MAGEAGASNVAAYPQARARPRAGLVLTGGGARAAYQVGVVKAVR
                     DLLGNPVKNPFPIVCGTSAGAINAASLAVFADDFTRAVAKLLEVWENMRCEHVYRTDF
                     VSIAKRGARWLLAMTLVSRGNPVSLLDNAPLRDMLAKSLAFERIQEHIDAGALYAVCV
                     TASGYTSGQSVSFFQGGSGIEGWERNQRIGAGVVLKLDYLMASSALPFIFPAVKVHRE
                     FFGDGSMRQIAPVSPALHLGADRVLIVGTGRQSLDQARARSSIYPSLAQIAGHALNSI
                     FLDSLSVDIERLERINRTVRLIPPERLEGSQLMLRPVRVLFITPSQPIERIAARFVQE
                     LPRAVRVVLRPTGALSRSGSNLASYLLFEESFCRALIDLGYQDTLAREAEVREFFSES
                     "
     gene            complement(8114..10441)
                     /locus_tag="AC16O9_09455"
     CDS             complement(8114..10441)
                     /locus_tag="AC16O9_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR6516881.1"
                     /translation="MFPRVEEAPVDEQLKQNALEYHRLPRPGKISVLPTKQLTSQRDL
                     GLAYSPGVAAACDEIVRDPAQAMQLTSRANLIAVITNGTAVLGLGQIGPLAAKPVMEG
                     KAVLFKKFAGIDCFDIEINERDPEKLVEIIASLEPTFGGINLEDIKAPECFIVERKLR
                     QRLHIPVFHDDQHGTAITVGAALLNGLKVVGKDLAKVQLVVSGAGAAALACLDLLVKM
                     GLPPEHITVTDIKGVVYRGRKEEMDPDKERYAKDTKARKLDDVIAGADVFLGLSAGGV
                     LKAEMVKKMAERPLILALANPEPEIRPELAKEVRPDCVIATGRSDYPNQVNNVLCFPF
                     IFRGALDVGATTINDEMELAAVRAIAELAQAETSEQVALAYGIETISFGPEYLIPRPF
                     DPRLISRVAPAVAWAAMDSGVATRPIDDLAAYRESLAQFVYHSGLIMKPLFAAAKTMS
                     NKRIVYAEGEDERVLRAVQIVVDEGLAQPTLIGRPQVIEQRIERLGLRVRAGKEFDLV
                     NPEFDPRYRDYWSTYHRLTERKGVSPEYAKIEMRRRHTLIGAMLMHKGEADGMLCGTF
                     GTYALHLHYVDQVIGLLPGIGHYAAMNALVMPNRTVFICDTYVTPDPDAAHIAEMTML
                     AAEEVKRFGLVPKVALLSASNFGSLELPSMRKMQRALALLHERAPDLEVEGEMHGDAA
                     LSEEIRLRVFPHSRLRGPANLLIMPTLDAANIAFNLLKTAAGDGVTVGPILLGASRAV
                     HILTPSATVRRLVNMTALAVVDANAPRPGVEPSLF"
     gene            10488..11663
                     /locus_tag="AC16O9_09460"
     CDS             10488..11663
                     /locus_tag="AC16O9_09460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6516882.1"
                     /translation="MNSSRLLAARRAADIEPFQVMDVLARAHALEAAGRHVVHMEIGE
                     PDFTAPDAVVEAGVRALRDGRTAYTAALGLPALREAIAAHYARAFGAALDPARVAVTA
                     GASGGLLLALALYVDPGDEVLVPDPGYPGYRHFVRAFEGVPRPLALSPRAQYQPTLEG
                     VRRAWGPRTKAFVLGSPSNPTGTIADAAELARIAQFVSERGGVLIADEIYQGLVYGAP
                     PVTALALPGEVVLVNSFSKYFCMTGWRLGWVVLPAARVRDFEKLAQHFFICAPSAAQH
                     AAVAAFAPHTLAVLEERRAQFERRRDLLVPALEHAGLQVPAPPRGAFYVYADCSRFGG
                     DARGFALELLEQAGVAATPGLDFGAHGTAHCLRFAYTRGLGELEEGAARIARFCAAR"
     gene            complement(11689..12387)
                     /locus_tag="AC16O9_09465"
     CDS             complement(11689..12387)
                     /locus_tag="AC16O9_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6516883.1"
                     /translation="MVALDNMTRIFSLARGLFALVGLAAVLVVALPAPRQAVLLELAR
                     LVPQAEAHGTDPVPVAAHEDIDAAAGPMPVAQVEAAHEREQRAVAEFIARRYRVAEAA
                     SSAFVSTAYRVGRELSVDPLLILAVMAVESRYNPVAESSMGAKGLMQVIPRYHPEKLL
                     EHGGEHALLDPDVNIHVGAQILREYMRRFGETQAALQKYAGAFDEPTAQYSGKVLAEM
                     ARLQQFLERALRDA"
     gene            complement(12751..13032)
                     /locus_tag="AC16O9_09470"
     CDS             complement(12751..13032)
                     /locus_tag="AC16O9_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like protein 2"
                     /protein_id="HIR6516884.1"
                     /translation="MAKKKAKKGGKKKKKKKSSKKKKKAVKKSSKKKAVGRKKKSAKK
                     KKAKPAKPKASSAGRMTVAASPASSAATPSAQTALNPAAAWPFPTGTRP"
     gene            complement(13151..13402)
                     /locus_tag="AC16O9_09475"
     CDS             complement(13151..13402)
                     /locus_tag="AC16O9_09475"
                     /inference="COORDINATES: protein motif:HMM:NF021873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR6516885.1"
                     /translation="MHRVTACKVEDDYKLWLRFEDGLEGSVFLGNLLEIGAFKLWRDV
                     REFEKVSVDPESATVTWEGGIRLDPDILYQDLAARGARE"
     gene            complement(13423..14565)
                     /locus_tag="AC16O9_09480"
     CDS             complement(13423..14565)
                     /locus_tag="AC16O9_09480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR6516886.1"
                     /translation="MLKFEDDEAKPNAISGLAVQPTSGFMPGAGGKEERFAAGNAFRR
                     ITVEDKRIINSKADVNQLVPFKYKWAWEKYIAACANHWMPQEINMSRDIALWKDPNGL
                     TADERRIVTRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQ
                     YIVESLDLDEGEVFNAYHEVGSIRDKDEFLVPFIDTLTDPAFRTGTPEADQQLLKSLI
                     VFSCLMEGLFFYVGFAQILSLGRQNKMTGSAEQYMYILRDESMHCNFGIDLVNQIKLE
                     NPHLWTARFREELQELFRRAVELEYRYAEDTMPRGVLGLNAPMFKEYLRFIANRRCQQ
                     IGLDALYPGATNPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD"
     gene            complement(14565..17414)
                     /locus_tag="AC16O9_09485"
     CDS             complement(14565..17414)
                     /locus_tag="AC16O9_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR6516887.1"
                     /translation="MQIAQESPATGPVASATVAGSSSTIRSAYSDYKIIRRNGAVVGF
                     EPAKISVAMTKAFLAVNGSQGAASARIRELVARLTEQVVGTLQRRQPQGGTVHIEDIQ
                     DQVELALMRSGEHDVARAYVLYREERARARAAEKAGRQSPEAVLHVVDGGARRPLDLE
                     ALRRLLETACEGLDGAAAPAAILETTLRDLYDGVPMEEVRKSAILAARALIEKDPAYS
                     YVTARLLLNTIRLEVLGEEASQQAMATRYAEYFPEFVRRGIEAELLDPELAKFDLARI
                     GRALKAERDLSFGYLGLQTLYDRYFLHVRGRRIELPQAFFMRVAMGLALREKDREARA
                     IEFYDVLSTFDFMSSTPTLFNSGTQRSQLSSCYLTTVADDLTGIYDAIKENALLAKYA
                     GGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLETW
                     HLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMENAEWTLFSPSDVPELHDK
                     VGRAFEEAYLRYEDKAARGELKLHKRLPAVQLWRKMLSMLFETGHPWITFKDPCNLRS
                     PQQHVGVVHSSNLCTEITLNTSADEIAVCNLGSVNLLAHMGERGLDFEKLKRTVRTAM
                     RMLDNVVDINYYAVDKAKNSNLRHRPVGLGIMGFQDCLHRLRIAYASPEAVAFADRSM
                     EMVAYCAYWASTELAEERGRYSSFKGSLWDRGILPQDSLDLLAAERGGYVEVDRSGVL
                     DWEPLRSRIRAHGMRNSNCIAIAPTATIANIVGVSASIEPTYQNLYVKSNLSGEFTVV
                     NEFLVADLKRAGLWDEVMIADLKYFDGNLARVDRAPAALRKLYATAFEVEPRWLVEAG
                     ARRQKWIDQSQSLNIYMAGASGKRLDETYKLAWIRGLKTTYYLRSMGATHAEKSTSKA
                     GQLNAVPSDGGVPVVAGAEEAKFCAIDNPECEACQ"
     gene            complement(17640..18188)
                     /gene="ampD"
                     /locus_tag="AC16O9_09490"
     CDS             complement(17640..18188)
                     /gene="ampD"
                     /locus_tag="AC16O9_09490"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR6516888.1"
                     /translation="MSIGPDGLLRGARYIASPNCDARPRGAAVRLLVLHSISLPPGKY
                     GGDAVERLFTNRLDAGAHPYFRSLAQLRVSAHFFVRRDGALQQFVPVHRRAWHAGASR
                     WRGLERCNDYAVGVELEGLEGRAFAAAQYRCLARLARALAAQLPLTACAAHSDVAPRR
                     KRDPGARFDWPRFYAGLARHNI"
     gene            complement(18172..19551)
                     /locus_tag="AC16O9_09495"
     CDS             complement(18172..19551)
                     /locus_tag="AC16O9_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6516889.1"
                     /translation="MRRAERKPVQRERRVLVVDDEPDIRELLELTLAKMGLGVVAVGS
                     LAEARDTLRDARFDLCLTDMRLPDGEGLDLVRHISGLPGDLPVAVITAYGSAENAVAA
                     LKAGAFDYVSKPVGLEQLRALVKSALSLPDQADPPARDLPLLGGSAPLDQVRKLIAKL
                     ARTQAPVHVSGESGSGKELAARLIHGEGARRERPFVPVNCGAIPETLMESEFFGYKKG
                     AFTGADHDRDGFFQAANGGTLFLDEVADLPLAMQVKLLRAIQEKRVRKVGATQEEPVD
                     VRIISATHQNLARLVESGRFRQDLFYRLNVIELKMPPLRDCREDIPLIAASILERIAR
                     ANGAAPARLSAAALVELAQYDFPGNIRELENILERATALSGGAEIGADDLMLRPVAAE
                     DSGARAGEGALGAPLPEYLDRVEREAILEALARTQFNRTAAAKLLGITFRTLRYRMQR
                     LGITDEHRP"
     gene            complement(19548..21143)
                     /locus_tag="AC16O9_09500"
     CDS             complement(19548..21143)
                     /locus_tag="AC16O9_09500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6516890.1"
                     /translation="MATLAYAPLFAREDRPPESFWISLQYFNLYRILVALLFLGATYV
                     AYEPINFGAHDMRLFRAVCVGYLILGIALQFVVRSVHERFNLQLSAHALLDVLVIPLL
                     MYASGGMRSGLGVMLLISLTGAGLVAPLRLTFLYAALAAIAVLLEETYWVLALDLPSA
                     SVVPAGLLSMGYFAIAGATGWLAQRLAANERLARERGRALANQTRVNQLVIADMQDGV
                     VLLDRQGRVVEHNPQSMRLLGMDVLRGLPLAAVLPGLVDRWRAWRDAGGRPAVSVDLE
                     ARGKEVRARLLDTGTEDQFTVVFVEDMTRSREQAQQLKLAALGRLTANIAHEIRNPLA
                     AISHAAELLAEERRGQDRGRLTRIIHDNTLRLERLVTDVLQLNRRDRIAAERIALAAW
                     LPPFIEEFAANESLPAQRFVIDTGRDAWVEFDPEHLRQVLWNLLRNAVRHARAEPGAV
                     RIAARAFGDKVELSVADNGPGVAPADQGQLFEPFFTTDSKGTGLGLYLARELAAANGA
                     LLEYVNDLPGAHFRIVCRGGAHA"
BASE COUNT         3204 a         7381 c         7193 g         3464 t
ORIGIN      
        1 ccaccatggc cgcgctcaac cgtcacaaca agacgagcca cctcgagaag ccgacgctgt
       61 tgctggaatt ccacggcacc gaggccagcg tcgtcgagca ggccgagatg gtgcaggaga
      121 tcgccaagga gaacggcggc ctgggcttcc agtgggccac caagcccgag gagcgcacca
      181 agctgtggga ggcgcggcac aacgcctatt tcgcctgcct gcagctaaag cccggctcgc
      241 gcgccatttc caccgacgtc tgcgtgccga tctcgcggct cgccgagtgc atcgtcgaga
      301 cgacccggga catcgagaag gcctcgatgc cgatcccgct cttcgggcac gtcggcgacg
      361 gcaacttcca cctcgtgatc ctggtggacc cggccagcgc gtcggacatg gaggaagcca
      421 aggcgctcaa caagcgcctg gtgaagcgcg cgctcgccat ggagggcacc tgcacgggcg
      481 agcacggcat cggcatgggc aagcaggcct cgctcaagga ggagctcggc gaggccgtcg
      541 acctgatgcg cgacctgaag cgcaccttcg acccggagaa cctgctcaac cccggcaaag
      601 tggtgccgtt gcagtgagcg cggaagcgag gctgaagaag ctggggatcg acctcggccc
      661 ggtttccgcg ccggtcgcca actacgtcaa cgccgtgcgt accgggaacc tgctgttcct
      721 ctcaggcaag ggtccgcgcg ccgtcgacgg taagcgcccg cagggcaaag tcgggcgcga
      781 gtacaccgcc gaacaggcgt accagcacgc gcgcaccgtg ggcctggacc tgctggcggt
      841 aatgcgggcg gagctcggca gcctggacaa ggtggcgcgc gtagtgaagc tcctcggcat
      901 ggtcaacgcc gtgcccgaat tccaggacca ccccaaggtg atcaacggct gctcggatct
      961 cttcgtcgag gtgctgggcg acgccggcaa gcacgcccgc tcggcggtcg gcatggggtc
     1021 actgcccatg ggcatccccg tcgagatcga ggtgatcgtc gaagtcaaat agggacagac
     1081 ctcatttttc gcatggcgaa aaatgaggtc tgtccctatt taaatggtca tgatggtctt
     1141 gcggtagaac ttcagttcct cgatcgagtc gaggatgtcg gccatggcct cgtgcttgct
     1201 ctccttcttc aggcccttgg ccgcctcggg ctgccagcgc cggaccagct ccttcagcgt
     1261 cgagacgtcg aggttgcggt agtggaacca ggcctcgagc ttcggcatcc agcgcgcgag
     1321 aaagcgccgg tcctggcaga tcgagttgcc gcacatcggc gatacgcccg agggcacgtg
     1381 cgcggcgagg aacgccagca tctgcacctc ggccgtcgcc tcgtccaggc gcgaagcctt
     1441 gaccttgtcg accaggccgg acttgccgtg cacgcccgtg ttccatgagt ccatcgccgc
     1501 cagcacctcg tcgggctggt gcacgacgag caccgggcct tcggcgacga tgttgagctg
     1561 cgcatcggtg accagcagcg cgatctcgag gatgcggtcg gtgtcgggag cgagcccgct
     1621 catttccatg tcgatccaga tcaggttgtt cgcatccgca ggcatgacgt attctctcat
     1681 ggcgccatga acgctttctc ctggatcttc ctcgctgcgc tcgccgcggc gacgctcgcc
     1741 cggctgtggc tcgcgcagcg ccaggtggcg cacgtgcgcg cgcaccgcga tgcggtgccg
     1801 gccgagttcg ccgatgcgat cccgctttcc gcgcaccaga aggccgcaga ctatacggtg
     1861 gccaaggcgc gcctcggaat gctcgatgtg ctgctcggcg cggcggtgct cctcgcgcta
     1921 acgctcggcg gcctgctgca gcggatgtcg gaattggcgg cccgggtgtt cgatcacggg
     1981 ggcctcgcgc acggcgtggc gctgatcgcc gccgccgtgg tgctgctctc ggcgctcgag
     2041 ctgccgctct cggcctaccg cacgttcgtc atcgaggcgc gcttcgggtt taaccgcatg
     2101 agcctcggcc tgttcctcgc cgacctcgcc aagcacgcgc tgctcggcgc cgtgctcggc
     2161 ctgccgctgc tggcgctggt gctgtggctg atgggccgca tgggcgacgc gtggtggctg
     2221 tacgtctggc tggcctggat cggcttcaac ctgctggtgc tgctcgccta cccgaccttc
     2281 atcgcgccgc tgttcaacaa gttctcgccg ctcgaggatc ccgcgctcgc cgggcgcatc
     2341 gagcagctgc tcgcgcgctg cggcttcacc tcgaacggcc tgtacgtgat ggacggctcg
     2401 cggcgctcgg cgcacggcaa cgcctacttc accggcttcg gcgccgccaa gcgcatcgtg
     2461 ttcttcgaca cgctgctcgc gcgcctggcg ccggccgagg tcgaggcggt gctcgcgcac
     2521 gagctcggcc actaccggcg ccaccacgtc tggaaacgca tcgcgttgct gttcgccctg
     2581 tcgctcggcc tgctgtggat tctgggccgg ctgatcgacg agccgtggtt ctacgccggg
     2641 ctgaacgccg gagcgcccgg cgacgccatg gggctgctgc tcttcgtgct ggtaatgccg
     2701 gtattcacgt ttttcctgca gccgctgacg agcctgtact cccggcgcca cgagttcgag
     2761 gcggattcct acgccgctgc gcacgccagc gccgcggagc tggtgcgcgc gctggtgaag
     2821 ctctaccagg acaacgccgc cacgctgacg ccggacccgc tgcactcggc gttctacgac
     2881 tcgcacccgc cggctgcgcg gcgcatcgcg cggctgcaag cgctcgccta ggaggcgcca
     2941 tgaacgagct cgcggcgaag aagtgcaagc cctgcgaggg cggcgtcgcc ccctttagcg
     3001 acgcccaggc gcgcgacatg ctgaagcagc ttaggggctg gagcctcgag gacggcaagc
     3061 tggtgaagct ctatcccttc ggtaactacc accacaccat ggcgttcgta aacgcgctcg
     3121 cctggatctc gcaccgcgag gaccaccacc cggacctcga ggtcagctac aacaagtgcc
     3181 gcgtcagcta ctcgacgcac gcgctgaacg ggctgtcgga gaacgacttc atctgcgcgg
     3241 cgaagtgcga cgcgctgttc gagatctagt gcggcgcgtc ttcagctcgt tcgacctggt
     3301 tgccgtgcat catgcgcgga acatgctcga ggccgagggc gtgcgcgccg tagtgcggaa
     3361 cgagcggctc gcctcagcga tgggcgagct gccgccggcc gagtgccagg cggaggtctg
     3421 ggtgctggac gaagccgacg cagcccgtgc ggagcagctg ctgcgcgagg gcgcgcgcgt
     3481 cccggcgggc ccgccgtgga cctgcgccgg atgcggcgag cgcatcgagc cgcagttcac
     3541 gcagtgctgg cactgcggcg ccacgcggcg cgactagagc gcgccggcga gcgctaccag
     3601 gaccagcagg aacacccaca ccacgagcgc gcgccacacc agcccgacca cgccgtcgag
     3661 aaacggcgcg tcggcggcct cgcccacgcc cagctcggga cgcacctcca cgccctcggg
     3721 cgccgagagc ggcatgccca ggcgcacgcc cagcgccccg gcgcccgcgg cgagcaccac
     3781 gccggcatcc gggtcgggcc aggccagcgc ctgcgtgcgc cagcagtaga ccgtgtcctc
     3841 gaaatcgccc accaccgcga acgacgccgc ggtgagccgc accgcaggcc actcgagcag
     3901 ccgcccgacc tgctcggcga agccgccgaa cgccccgagc ccgccccagc gccggcgcag
     3961 gtacgccgcg agccggtaga ggatggcgcc gctcggcccg ggcagcagca cgaaccagaa
     4021 caacacgccg aacacgtggc ggtgcgaggc gaccagcgcc tcctcgatgg cgaggcgaat
     4081 cacctcctcg cggctgcggt gctgcgccgg ctcgccgcgc cactcgccga tcagcgcgcg
     4141 cgcgcgctcg aggtccccgg tgcggatcgc gagctgcagg tcggtgaagt agtggctgaa
     4201 ctggcgaaag ccgagggtga aatacagcgc gacgatattg aaggcgaacg cgagcagcgg
     4261 atgtagccat agcagcagaa agtagagcgc gagcgacgcg gcgagcacgg gcagtaccgc
     4321 cacgagccag gcggtggtgc cttggcgccc ttcgccggcg ttgaacgagc gctccagcca
     4381 ctcggcgccc cccgccagca acgcgaagta ggccttgcgc tcgccgagcg ggcgccactg
     4441 ctcgagcagc aacgcgacga tgacagcgat ggcgctcacc cgccgccccg ccccaggaat
     4501 cgatacaggt tgaccagcat gccggcggtg gcgccccaga tgtagtaatc cccgtacggg
     4561 atggcgtaga acggcaccgt gcgcccctgg aactcgcgct ggtggcgctg gtggttggcc
     4621 gggtccagca ggaacgcgag cggcacctcg aacgcctcgt ccacctcgtg cgcgtcaagg
     4681 cgcagcgtga aaggcggcgc caccaggccc accaccgggg tgacccggta atcggtgcgc
     4741 gtgtggtagt ccgagagccg cccgatcacc tccacgcgcg ccggctcgag cccgatctcc
     4801 tcctcggcct cgcgcagcgc cgtggcctcg gggctcgcgt cgcccggctc ggcgccgccg
     4861 ccgggaaagg caatctggcc cgagtggttc ttgaggtggg tggtgcgacg cgtgagaagt
     4921 acactcagcg cctcgggcct tgtcacgatc gggaccagga cgctcgcccg gcgccgcgcc
     4981 gcagcacgct cggcgtcgat accctggtcg ccgtagagca tcgccaccgg ctcgcccgcg
     5041 gcaaagcgcc gccgtagccg gtcggggtcg agcccgggcg gcgtgtcgtg attcagcgtc
     5101 gcccgcatgc ggaatatttc gtgcgcatct cgcgtttgcc tcccagccga gatgataatc
     5161 gctcccggcc ccatccaacg atcgaggagg atcacatgaa aagaaccatc ctgggcttcg
     5221 tgctcgccct ggcaagcgca tcggcgttcg ccttccactg cccgaaggac atgaagaaga
     5281 tcgacgaggc aatggccaag aacccgagct tgtcggcgca gcagatggcc gacgtgaaga
     5341 agtaccgcgc cgagggcgag gccctgcaca aggccggcaa ccaccagggc tcggtcgacg
     5401 cgctcgccaa ggcgatgaag atcctcagca tccagtagcg cgggccgtcg gccccggaaa
     5461 aggaaaacgc cgcccgcggg cggcgttttc catcgtgcgc cggtggcctc ttacggcagc
     5521 aggatcaccg agccggtggt cttgcggccc tcgaggtcgg tgtggcactt ggccgcctcg
     5581 gcgagcttgt agcgcgccga gatctcgatc ttcaccttgc ccgatttcac cgcatcgaac
     5641 accgaggccg agcgcgcctc gaggtcggca cgcgcggcga tgtaggtcac gagcgtcggc
     5701 cgcgtcatgt agagcgagcc cttctgcgcg aggacctgcg tgttgaacgg cggcaccggc
     5761 ccggaggcgt tgccgaagat tacccacagg ccaaggggcc gcaggcagtc gagcgaccct
     5821 tcccaggtgg tcttgccgac cgagtcgtac accacgggca gcttcttgcc gccggtgatc
     5881 tccttcacgc gctcgacgaa gttctccttc gtgtagtcga tcacgtgctc gcagccgtgc
     5941 gccttggcga tcttcgcctt ctccggggag ccgacggtgc cgatgacggt gacgcccagc
     6001 gccttcagcc actggcaggc catcagcccg acgccgccgg cggcggcgtg ccacagcacc
     6061 gtctcgcctt tcttgaccgc gtaggtactg tggatgaggt actggaccgt catgcccttc
     6121 agcatgaggc aggcggcctg ctcgtcgctg atgccctcgg gcaccttcac gacgcgatcc
     6181 gcgggcagga gccgctcggt gcaataagag ccaacctgtc cggtgtaggc gacgcgatcc
     6241 cccttcttga gaccgtccac gcccgggccg acggactcga ccacgcccgc gccctcgttg
     6301 cccgccgcgc tcggcaactg catcggatac aggcccgagc gctggtaggt gtcgatgaag
     6361 ttgacgccga tcgccgtgtg ccggatgcgc acctggccct gccccggctc gcccacctgc
     6421 acgtcgtcca gctgcaggac ttcgggcccg ccggtcttgt ggaatcgcac tgccttgctc
     6481 atcgcttgtg cctcctcgtc agtaatgcgt tcattccttc ggcttgttct cgtcgtccgc
     6541 ggccggcggc ggctcgctcg ccggcggctt gcccgccatc gcctgctgca tcaccgccca
     6601 cggccagagc atgccgctcg cggcctggtt ggcgctcgcc gcggcgagct cggcgagcgc
     6661 gctcggcggc gcacccgccg gggcgccggc acccatggcg agcagagccg agcgctgcac
     6721 ctccaggccc tgcgccgcga agcgcaccat gttcaggttc acctcgagcc acgactcgat
     6781 cgaacgcagc tcggcgatgc gcttgtccac ttccttcgga tccagcgtcg gcaccgccat
     6841 gccgggcact ggcaggccga gcggccccca cagacggcgg aagatctcga acgggtcctg
     6901 cggggggttc gattctgcca tggacggcct cacgattcgg aaaagaattc cctgacctcg
     6961 gcttcgcgcg ccagggtgtc ctgataaccc aggtcgatga gcgcgcggca gaacgactcc
     7021 tcgaacagca ggtagctcgc caggttggag cccgagcgcg acagcgcgcc ggtcggccgc
     7081 agcacgacgc gcaccgcgcg cggcagttct tgcacgaagc gcgccgcgat gcgctcgatc
     7141 ggctgcgacg gcgtgatgaa cagcacccgc accgggcgca gcatcagctg cgagccctcc
     7201 agccgctccg gcggtatcag ccgcaccgtg cggttgatgc gctccagtcg ctctatgtcc
     7261 accgataggc tgtcgaggaa gatggagttc agtgcgtggc cggcgatctg cgcgagcgac
     7321 gggtagatgc tggagcgtgc gcgcgcctgg tcgaggctct gccggcccgt gccgacgatc
     7381 agcacgcggt cggcgcccag gtgcagcgcc gggctcaccg gcgcgatctg gcgcatcgag
     7441 ccatcgccga agaactcgcg gtgcaccttg accgcgggaa agatgaacgg cagcgccgag
     7501 gaggccatca ggtagtccag cttcaacacc acccccgcgc cgatgcgctg gttgcgctcc
     7561 cagccctcga tccccgagcc gccctggaag aacgacacgc tctgccccga ggtgtagccc
     7621 gacgcggtga cgcacaccgc gtacagcgca cccgcgtcga tatgctcctg gatgcgctcg
     7681 aaggccaggc ttttcgccag catgtcgcgc agcggcgcgt tgtcgagcag ggagaccggg
     7741 ttgccccgcg acaccagcgt catggcgagc aaccagcgcg caccgcgttt cgcgatgctc
     7801 acgaagtccg tgcggtagac atgttcgcaa cgcatgttct cccacacctc gagcagcttg
     7861 gccaccgcgc gcgtgaagtc gtcggcgaac actgcgagcg acgcggcgtt gatcgcgcct
     7921 gccgatgtgc cgcagacgat gggaaagggg ttcttcaccg gattgccgag caggtcgcgc
     7981 accgccttca ccacgcccac ctggtaggcg gcccgcgcac cgccgccggt cagcaccagg
     8041 ccggcgcgcg gccgcgcgcg cgcttgcggg tacgccgcca cgttgctcgc gcctgcctcg
     8101 cccgccatgg ccgctagaac aggctcggct ccacgcccgg gcgcggcgcg ttggcgtcga
     8161 cgacggcgag cgccgtcatg ttgacgaggc ggcgcacggt ggccgaagga gtgaggatgt
     8221 gcaccgcccg tgatgcgccg agcaggatcg ggccgacggt gacgccgtcg ccggccgccg
     8281 tcttcagcag gttgaaggcg atgttggctg cgtccagcgt cggcatgatg agcaggttgg
     8341 ccggtccccg caggcgcgaa tgcgggaaca ccctgaggcg gatctcctcc gaaagcgcgg
     8401 cgtcgccgtg catctcgcct tccacctcca ggtccggcgc gcgctcgtgc aggagggcga
     8461 gcgcgcgctg catcttgcgc atcgacggca gctcgagcga gccgaagttc gaggccgaga
     8521 gcagcgccac cttgggcacg aggccgaagc gcttgacctc ctccgcggcg agcatggtca
     8581 tttccgcaat gtgcgcggcg tccgggtcgg gcgtgacgta ggtgtcgcag atgaacacgg
     8641 tgcggttggg catcaccagc gcgttcatcg ccgcatagtg tccgattccg ggcagcaggc
     8701 cgatgacctg gtcgacataa tgcaggtgca gcgcatacgt gccgaacgtg ccgcacagca
     8761 tgccgtcggc ctcgcccttg tgcatgagca tcgcaccgat cagcgtgtgg cggcggcgca
     8821 tctcgatctt ggcgtactcg ggcgacacgc ctttgcgctc ggtgagcctg tggtaggtgc
     8881 tccagtagtc gcggtagcgc gggtcgaact ccgggttgac cagatcgaac tccttgcccg
     8941 cgcgcacgcg caggccaagg cgctcgatcc tctgctcgat gacctgcggc cgcccgatca
     9001 gcgtcggctg cgccaggccc tcgtcgacga cgatctgcac cgcgcgcagc acgcgctcgt
     9061 cctcgccctc ggcgtacacg atgcgcttgt tggacatggt cttggcagcc gcgaacagcg
     9121 gcttcatgat caggcccgag tggtacacga actgcgccag cgactcgcgg tacgcggcga
     9181 ggtcatcgat cgggcgcgtc gccacgcccg agtccatggc cgcccaggcc accgccggcg
     9241 ccacgcgcga aatgaggcgc ggatcgaacg gccgcgggat caggtactcc gggccgaagc
     9301 tgatcgtctc gatgccgtag gcgagggcca cctgctcgga ggtctccgcc tgcgccagct
     9361 cggcgatagc gcgcaccgcc gcgagttcca tctcgtcgtt gatggtcgtg gcgccgacgt
     9421 cgagcgcacc gcggaagatg aacggaaagc acaggacgtt gttcacctgg ttcgggtagt
     9481 ccgagcggcc ggtggcgatg acgcaatcgg gacgcacctc tttcgccagt tccggccgga
     9541 tctcgggctc cgggttggcg agcgcgagga tcagcggccg ctcggccatc ttcttcacca
     9601 tctcggcctt gagcaccccg cccgccgaca ggccgaggaa cacatcggcc ccggcgatga
     9661 cgtcgtcgag cttgcgcgcc ttggtatcct tggcgtagcg ctccttgtcc gggtccatct
     9721 cttccttgcg gccccggtac accacgccct tgatgtcggt gacggtgatg tgttcaggcg
     9781 gcaggcccat cttcaccagc aggtcgaggc aggcgagcgc cgcggcgccc gcgcccgaga
     9841 ccaccagctg aaccttcgcc aggtctttgc cgaccacctt cagtccgttc agcagcgcgg
     9901 cgccgacggt gatggcagtc ccatgctggt cgtcgtggaa cacgggaatg tggagccgct
     9961 gccgcagctt gcgctcgacg atgaagcatt cgggcgcctt gatgtcctcg aggttgatgc
    10021 cgccgaaagt cggctcaagg ctggcgatga tctcgacgag cttttccgga tcgcgctcgt
    10081 tgatttcgat atcgaagcag tcgatgccgg cgaacttctt gaacagcacc gccttgcctt
    10141 ccatcaccgg cttcgccgcg agcggcccga tctgcccgag accgagcacg gccgtgccgt
    10201 tggtgatgac ggcgatgagg ttggcgcgcg aggtgagctg catcgcctgc gccggatcgc
    10261 gcacgatctc gtcgcacgcc gccgccacgc cgggcgaata ggcgaggccg agatcgcgct
    10321 ggctggtgag ctgcttggtg gggaggaccg agatcttccc ggggcggggc aggcggtgat
    10381 attcgagtgc gttctgcttg agttgctcgt ccaccggtgc ctcctcgacg cggggaaaaa
    10441 ttatatcaac ctgtccgtat aatccccggc tccagagcgc gtacgccttg aattcctccc
    10501 gcctgctggc ggcgcgccgc gccgccgaca tcgagccgtt ccaggtcatg gatgtgctcg
    10561 cgcgcgcgca tgcgctcgag gctgccggcc gccacgtggt gcacatggaa atcggcgaac
    10621 cggacttcac cgcgcccgat gcggtggtgg aagccggcgt gcgcgcgctg cgcgacgggc
    10681 gcacggcgta caccgcggcg ctcggcctgc cggccctgcg cgaggcgatc gccgcgcact
    10741 acgcgcgtgc ctttggcgcc gcgctcgatc cggcgcgcgt cgccgtgact gcgggcgcct
    10801 cgggcggcct gctgctcgcg ctggcgctgt acgtggaccc gggcgacgag gtgctggtgc
    10861 ccgatcccgg ctatccgggc taccgccatt tcgtgcgcgc gttcgagggc gtgccgcggc
    10921 cgctcgcgct ttcgccgcgg gcgcagtacc agccgacgct cgagggcgtg cgccgcgcct
    10981 ggggcccgcg caccaaggcg ttcgtgctcg gctcgccctc caacccgacc ggcacgatcg
    11041 ccgacgcggc ggagcttgcg cgcatcgcgc agttcgtgtc cgagcgcggc ggcgtgctga
    11101 tcgccgacga gatctatcag gggctggtat acggtgcgcc cccggtcacc gcgctcgcgt
    11161 tgcccggcga ggtcgtgctc gtcaacagct tttcgaaata cttctgcatg acgggctggc
    11221 gcctgggatg ggtggtgctg cccgccgcac gcgtgcgcga cttcgagaag ctggcgcagc
    11281 acttcttcat ctgcgcgccg agcgccgcgc agcacgcggc agtcgcggcc tttgcgccgc
    11341 acaccctcgc cgtgctggaa gagcgccgcg cgcagttcga gcgccgccgc gacctgctgg
    11401 taccggcgct cgagcacgcg gggctgcagg tgcccgcgcc gccgcgcggc gcgttctacg
    11461 tgtacgcgga ctgcagccgc ttcggcggcg acgcccgcgg cttcgccctc gagcttcttg
    11521 agcaggcggg ggtggcagcg acgccgggac tggacttcgg cgcccatggc acggcgcact
    11581 gcctgcgttt cgcctacacg cgcgggctcg gcgagctgga ggaaggcgcc gcgcgcatcg
    11641 cgcgcttctg cgccgcgcgc tagggtctgc ggcgcccgcc ggcgccgctc aggcgtcgcg
    11701 cagcgcgcgc tccaggaact gctgcaggcg cgccatctcg gccagcacct tgcccgagta
    11761 ctgcgcggtc ggctcgtcaa aagcccccgc gtacttctgc agcgcggcct gggtctcgcc
    11821 gaaacggcgc atgtactcgc gcaggatctg cgcgccgacg tggatgttga cgtccgggtc
    11881 gagcagcgcg tgctcgccgc cgtgctcgag gagtttctcc gggtgatagc gcgggatgac
    11941 ttgcatcagc cccttcgcgc ccatcgagct ctcggcgacc gggttgtagc gcgattcgac
    12001 ggccatcacc gcgaggatca gcagcggatc taccgacagc tcccgtccta cccggtacgc
    12061 cgtcgacacg aacgcgctgc ttgctgcctc ggcgacgcga tagcgccgcg cgatgaactc
    12121 ggccacggcg cgctgctcgc gctcgtgcgc cgcttccacc tgcgccacgg gcatcgggcc
    12181 ggccgccgca tcgatgtctt cgtgcgctgc caccgggacg gggtcggtgc catgggcctc
    12241 ggcctgcggc acaagccgcg caagttccag gagcacggcc tggcgaggcg ccggcagcgc
    12301 cacgacgagc accgccgcca gaccgaccag cgcgaacagt ccccgcgcga gggaaaaaat
    12361 tcttgtcata ttgtccagtg cgaccatctt gcacctccgt gtcccagccg tcgcctgctg
    12421 tgcccgctgg ccattttgct gcagtgcagc gacctggatc atatgatgaa ttacttaata
    12481 tcgtcaatag gttctgacgt tatgtgacat ttttggcatg cgacgcgaca tgaagaagtt
    12541 cgatctacga acttgttttc ccgcgtattt gaaccccggt agaatgcccg ctgagacatg
    12601 tgcccggcca gcccctcgac cgggaacaag ggaagcctcg cggaccggcc tcagaccttc
    12661 agttgcgccg aatgaccaag gcggtccgct gagcttcgag ggcctggcgg ggggcttgcg
    12721 ccccccgcca cattcccgct tggcgcccgg ttacggccgg gtgccggtcg gaaacggcca
    12781 cgcggccgcc ggattcagcg cggtttgcgc gctgggagtc gcggcggagg atgccggcga
    12841 tgctgccacc gtcatccgtc ccgcagagct ggccttaggt ttagcgggct tcgccttttt
    12901 tttcttggcg gatttcttct tgcggcccac tgccttcttc ttggatgact tcttaactgc
    12961 ttttttcttt ttctttgatg atttcttctt cttctttttc ttgccgcctt ttttggcttt
    13021 tttctttgcc atgactagct ccttgctggt agttgaacaa aagaagcccg gccatccctg
    13081 accggactat tcaaccgcgc gcgggaatcg cttcccgcag gcggactgaa ttcctcgccc
    13141 caccgcatgc tcactcgcgc gccccccgcg cggccaggtc ctggtacagg atgtccggat
    13201 cgagccggat gcccccttcc caggtcacgg tcgcggattc cggatccacg ctcacttttt
    13261 cgaactcgcg cacgtcgcgc cacagcttga acgcgccgat ctcgagcagg ttgccgagga
    13321 agacactgcc ctccaggccg tcttcgaacc gtagccaaag cttgtagtcg tcttccacct
    13381 tgcaggcggt cactcggtgc acttggcgtc tctcctctcg catcagtccc agctcagggc
    13441 gccgccagtc tggtactcga tgacccgcgt ctcgaagaaa ttgcgctcct tcttcaggtc
    13501 gatcatctcg ctcatccacg ggaacgggtt ggtcgccccc ggatagagcg catccagccc
    13561 gatctgctgg cagcgccggt tggcgatgaa gcgcaggtac tccttgaaca tcggcgcgtt
    13621 gagccccagc acgccgcgcg gcatggtgtc ttcggcgtag cggtactcca gctccaccgc
    13681 ccggcggaac agctcctgca gctcctcgcg gaagcgcgcg gtccacaggt gcgggttctc
    13741 gagcttgatc tggttgacga gatcgatgcc gaaattgcag tgcatcgact cgtcgcgcag
    13801 gatgtacatg tactgctcgg cggacccggt catcttgttc tgccggccga gcgaaaggat
    13861 ctgcgcaaag ccgacgtaga agaacaggcc ctccatcagg caagagaaca cgatcaggct
    13921 cttgagcagc tgctggtcgg cctcgggcgt gccagtcctg aacgccgggt cggtcagggt
    13981 gtcgatgaac ggcaccagaa actcgtcctt gtcgcggatc gagccgacct cgtgatacgc
    14041 gttgaacact tcgccctcgt cgagatcgag gctttcgacg atgtactggt aggcgtgggt
    14101 gtggatggcc tcttcgaacg cctggcgcag caggtactgc cggcactccg gcgccgtgat
    14161 gtggcggtag gtgccgagca cgatattgtt ggcggcgagc gaatcggcgg tgacgaagaa
    14221 cccgaggttg cgcgtgacga tgcgccgctc gtccgcggtg agcccgttcg ggtccttcca
    14281 cagggcgatg tcgcggctca tgttgatttc ctgcggcatc cagtggttgg cgcaggcggc
    14341 gatgtacttc tcccaggccc acttgtactt gaacggcacc agctggttga cgtcggcctt
    14401 ggagttgatg atgcgcttgt cctcgaccgt gatgcgccgg aacgcgttgc cggcggcgaa
    14461 gcgctcctcc ttgcccccgg cccccggcat gaagccgctg gtcggctgta cggcgaggcc
    14521 ggagatggcg ttcggcttcg cctcgtcgtc ttcgaacttg agcattactg gcaggcctcg
    14581 cactcggggt tgtcgatcgc gcagaacttc gcctcctcgg cgcccgccac caccggcacg
    14641 ccgccgtcgc tcggcaccgc gttcagctgc ccggccttgg aggtcgactt ctcggcgtgc
    14701 gtcgcgccca tggagcgcag gtagtaggtg gtcttcaggc cccggatcca cgccagcttg
    14761 taggtttcat ccagccgttt gcccgaggcg ccggccatgt aaatgttcag cgactgcgac
    14821 tggtcgatcc acttctggcg gcgcgcgccg gcctcgacca gccagcgcgg ctcgacctcg
    14881 aatgccgtgg cgtagagctt gcgcagcgcc gccggcgcgc gatcaacacg ggccaggttg
    14941 ccgtcgaagt acttgaggtc ggcgatcatc acctcgtccc acaacccggc gcgcttcagg
    15001 tcggccacca ggaactcgtt gaccacggtg aactcgcccg acaggttcga cttcacgtac
    15061 aggttctggt aggtcggctc gatcgaagcc gagacgccga cgatgttggc gatggtcgcc
    15121 gtcggcgcga tggcgatgca gttggagttg cgcatgccgt gcgcgcggat gcggctgcgc
    15181 agcggctccc agtcgaggac cccggagcgg tcgacttcga cgtagccacc gcgttccgca
    15241 gcgagcaggt cgagcgagtc ctgaggcagg atgccgcggt cccacagcga gcccttgaag
    15301 ctcgagtagc gcccgcgctc ctcggcgagc tcggtcgacg cccagtacgc gcagtaggcc
    15361 accatctcca tcgagcggtc ggcgaacgcc accgcctccg gcgaggcgta ggcgatgcgc
    15421 agccggtgca ggcagtcctg gaagcccatg atgccgaggc ccaccgggcg atgccgcagg
    15481 ttggagttct tcgccttgtc gacggcgtag tagttgatgt cgacgacgtt gtcgagcatg
    15541 cgcatcgcgg tgcgtacggt gcgtttgagc ttctcgaagt ccagaccgcg ctcgcccatg
    15601 tgcgccagca ggttcaccga gcccaggttg cacactgcga tctcgtcggc cgaggtgttc
    15661 agcgtgatct cggtgcacag gttggagctg tgcaccacgc ccacgtgctg ctgcgggctt
    15721 ctgaggttgc acgggtcctt gaacgtgatc cagggatgcc cggtctcgaa cagcatcgac
    15781 agcatcttgc gccacagctg caccgccggc aggcgcttgt gcagcttcaa ctcgccgcgc
    15841 gccgccttgt cttcgtagcg cagatacgcc tcctcgaacg cccgtcccac tttgtcgtgc
    15901 agctcgggga cgtcggaggg actgaacagg gtccactcgg cgttctccat cacgcgcttc
    15961 atgaacaggt ccgggatcca gttggccgtg ttcatgtcgt gcgtacgccg gcggtcgtca
    16021 ccggtgttct tgcgcagttc gagaaactct tcgatgtcga ggtgccaggt ctccaggtac
    16081 gagcacaccg cgccctttct cttgccgccc tggttcaccg ccaccgcggt gtcgttcacc
    16141 accttcagga acggcaccac gccctgcgac ttgccgttgg tgcccttgat gtgcgagccg
    16201 agggcgcgca ccggggtcca gtcgttgccc aggccgcccg cgtacttggc gagcagcgcg
    16261 ttctccttga tggcgtcgta gatgccggtg aggtcatcgg ccaccgtggt caggtagcac
    16321 gaggagagct gcgagcgctg cgtgcccgaa ttgaacagcg tcggcgtcga gctcatgaag
    16381 tcgaaggtcg acagcacgtc gtagaactcg atggcgcgcg cctcgcggtc cttctcccta
    16441 agcgccaggc ccatcgccac gcgcatgaag aaggcctgcg gcagctcgat cctgcgcccg
    16501 cgaacgtgca ggaagtagcg gtcgtagagc gtctgcaggc cgaggtagcc gaagctgagg
    16561 tcgcgctcgg ccttcagcgc gcggccgatg cgcgccaggt caaattttgc aagctcgggg
    16621 tcgagcagct cggcctcgat gccgcggcgc acgaattccg gaaagtactc ggcgtagcgc
    16681 gtcgccatcg cctgctggct cgcctcctcg cccagcacct cgaggcggat ggtgttcagg
    16741 agcaggcgcg ccgtgacgta gctgtacgcc gggtctttct cgatcagcgc gcgcgcggca
    16801 aggatcgccg acttgcgcac ttcctccatc ggcacgccgt cgtacaggtc gcgcagcgtg
    16861 gtctcgagga tcgccgccgg cgctgccgcg ccgtcgagcc cctcgcacgc cgtctcgagc
    16921 agccgccgca gcgcctcgag gtcgagcggc cggcgcgcac cgccgtccac cacgtgcagc
    16981 accgcctcgg gcgactgccg cccggccttc tccgcggcgc gcgcgcgggc gcgctcctcg
    17041 cggtacagca cgtaggcgcg ggcgacgtcg tgctcgcccg agcgcatcag cgccagctcg
    17101 acctggtcct ggatgtcctc gatgtgtacc gtgccgccct ggggctggcg ccgctgcagc
    17161 gtgcccacca cctgctcggt gagccgcgcc accagctcgc ggatgcgcgc cgaggccgcg
    17221 ccctggctgc cgttcaccgc cagaaacgcc ttggtcatcg ccaccgagat cttcgccggc
    17281 tcgaagccga ccacggcgcc gttgcggcgg atgatcttgt agtcgctgta cgcggaacgg
    17341 atcgtgctgc tggagccggc aacggtcgcg ctagccacag ggccggttgc tggactctct
    17401 tgcgcgatct gcatgtacct gcctcccttg cgttgccgcc cgctgcaggg cattcgatgc
    17461 aagctgcagc ggaaccacaa tatatagtgc tgaacctctg attgctaact atttctagtg
    17521 gccgcaatgt accacgcgga ttttattttg caagcggcgc aacatgccgc gcgccgtggc
    17581 gccggatttg cgcggtgcag cgtagccact aacagcggtg gaaacggaga ggccggctac
    17641 tagatgttgt ggcgcgcgag gccggcgtag aagcgcggcc agtcgaagcg cgcgcccggg
    17701 tcgcgcttgc gccgcggcgc gacgtcgctg tgcgcagcgc aggcggtgag cggcagctgc
    17761 gccgcgagcg cgcgcgccag gcgcgccagg caccggtact gcgcggcggc gaatgcgcgg
    17821 ccttccaggc cttcgagctc gacgccaacg gcgtagtcgt tgcagcgctc gaggccgcgc
    17881 cagcgcgagg cgcctgcgtg ccaggcgcgc cggtgcaccg ggacgaactg ctgcagcgca
    17941 ccgtcgcggc gcacgaagaa atgcgccgat acgcgcagct gcgccaggga gcgaaagtat
    18001 gggtgcgcac ccgcgtcgag ccggttggtg aacaggcgct cgacggcgtc gccgccgtac
    18061 ttgccgggcg gcaggctgat cgaatgcagt acgaggagcc gcaccgccgc gccgcgcggc
    18121 cgcgcgtcgc agttgggcga ggcgatgtag cgggcgccgc gcagcagccc gtcagggccg
    18181 atgctcatcc gtgatgccga ggcgctgcat gcggtagcgc agcgtgcgga aggtgatgcc
    18241 gagtagcttg gcggcggcgg tgcggttgaa ctgggtgcgc gccagcgcct cgaggatcgc
    18301 ctcgcgctcg acgcggtcga ggtactccgg cagcggcgcg ccgagcgcgc cttcgccggc
    18361 gcgggcgccg ctgtcttcgg ccgcgaccgg cctgagcatc aggtcgtcgg cccctatctc
    18421 ggcgccgccc gagagcgccg tggcgcgctc caggatattc tccagctcgc ggatgttgcc
    18481 cgggaaatca tattgcgcca gctcgacgag cgccgccgcc gacagccgcg ccggcgccgc
    18541 gccgttcgcc cgcgcgatgc gctcgaggat gctcgccgcg atcagcggga tgtcctcgcg
    18601 gcagtcgcgc aaaggcggca tcttcagctc gatcacgttg aggcggtaga acaggtcctg
    18661 gcgaaagcgc cccgattcca ccagccgcgc caggttctgg tgcgtggcgc tgatgatgcg
    18721 cacgtccacc ggctcttcct gcgtcgcgcc caccttgcgt acgcgctttt cctggatcgc
    18781 gcgcaacagc ttcacctgca tggcgagcgg caggtccgcc acttcgtcga ggaacagcgt
    18841 gccgccgttg gcggcctgga agaagccgtc gcggtcgtgg tcggcgccgg tgaacgcgcc
    18901 ctttttgtag ccgaagaact cgctctccat cagggtctcg ggaatcgcgc cgcagttcac
    18961 cggcacgaag gggcgctcgc ggcgcgcgcc ttcgccgtgg atcagccgcg cagcgagctc
    19021 cttgccgctg ccggactcgc ccgagacgtg caccggcgcc tgcgtgcgcg cgagcttggc
    19081 gatcagtttg cgcacctggt cgagcggcgc gctgccaccg agcagcggca ggtcgcgcgc
    19141 cggtggatcc gcctggtcgg gcagcgacag cgccgacttc accagcgcgc gcagctgctc
    19201 caggcccacc ggcttggaca cgtaatcgaa ggcgccggcc ttgagcgcgg cgaccgcgtt
    19261 ctcggcgctg ccgtacgccg tgatcaccgc caccggcagg tcgcccggca ggccgctgat
    19321 gtggcgcacc aggtcaaggc cttcgccatc gggcaggcgc atgtcggtca agcacaggtc
    19381 gaagcgcgcg tcgcgcaacg tgtcgcgtgc ctcggccagg ctgcccaccg ccaccacccc
    19441 cagccccatc ttcgccagcg tcagctccag cagctcgcgg atgtcgggct cgtcgtccac
    19501 caccagcacg cggcgctcgc gctgcacggg cttgcgctcg gcgcgcctca tgcgtgcgcc
    19561 ccgccgcggc agacgatgcg gaaatgcgcg cccggcaggt cgttgacgta ctcgagcagc
    19621 gcaccgttgg cggcggccag ttcgcgtgcg aggtacaagc ccaggccggt ccccttgctg
    19681 tcggtggtga agaacggctc gaacagctgc ccctggtcgg cgggggccac cccggggccg
    19741 ttgtcggcga cgctcaattc tactttgtcg ccgaaggcgc gcgccgcgat gcgcacggcg
    19801 ccgggctcgg cgcgcgcgtg gcgcacggcg ttgcgcagca ggttccacag cacctggcgc
    19861 aggtgttcgg gatcgaactc gacccaggcg tcgcgacccg tgtcgatcac gaaccgctgc
    19921 gccggcagcg actcgttggc ggcaaactcc tcgatgaacg gcggcagcca ggccgcgagc
    19981 gcgatgcgct cggcggcgat gcggtcgcgg cggttgagct gcagcacgtc ggtcaccagg
    20041 cgctccaggc gcagcgtgtt gtcgtggatg atgcgcgtca ggcggccgcg gtcctgaccg
    20101 cggcgctcct ccgcgagcag ctccgcggcg tggctgatgg cggcgagcgg attgcggatc
    20161 tcgtgcgcga tgttggccgt cagccggccg agcgccgcga gcttcagctg ctgcgcctgc
    20221 tcgcgcgagc gcgtcatgtc ctcgacgaac accacggtga actggtcttc ggtgccggtg
    20281 tcgagcagcc gcgcgcgcac ctccttgccg cgcgcttcca ggtccaccga caccgcgggg
    20341 cgcccgcccg cgtcgcgcca ggcgcgccag cggtccacca gcccgggcag caccgcggcg
    20401 agcggcagcc cgcgcagcac gtccatgccg agcaggcgca tcgactgcgg gttgtgctcg
    20461 acgacgcggc cctgccggtc gagcagtacc acgccgtcct gcatgtccgc gatcaccagc
    20521 tggttgacgc gcgtctggtt ggcgagcgcg cggccgcgct cgcgcgccag gcgctcgttg
    20581 gcggccaggc gctgcgccag ccagccggtg gcaccggcga tggcgaagta gcccatcgac
    20641 agcagccctg cgggcaccac gctcgccgag ggcaggtcga gcgccaacac ccaataggtc
    20701 tcctcgagca gcacggcgat cgcggcgagc gccgcgtaga ggaacgtcag ccgcagcggc
    20761 gccaccagcc cggcgccggt gagcgagatc agcagcatca cgcccaagcc gctgcgcatg
    20821 ccgccgctcg cgtacatgag cagcgggatg acgagcacgt cgagcagcgc gtgcgccgag
    20881 agctgcaggt tgaagcgctc gtgcaccgag cgcaccacga actgcagcgc gatgccgagg
    20941 atcaggtagc cgacgcacac ggcgcggaac agccgcatgt cgtgcgcgcc gaagttgatc
    21001 ggctcgtagg cgacgtaggt ggcgccgagg aacagcagcg ccaccaagat gcggtacagg
    21061 ttgaagtact gcagcgagat ccagaacgat tccggcgggc ggtcttcgcg cgcgaacagc
    21121 ggcgcatagg ccagtgtcgc cattacggct ggcgcggccc gagccgcgcg tgctcctcgc
    21181 tgcagtactc gccgccggcc gcgctgcgcg cctcggcgcg cggcaggtgc acgccgcagt
    21241 gc
//
LOCUS       DBNHKE010000141        28233 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1293809, whole genome shotgun sequence.
ACCESSION   DBNHKE010000141 DBNHKE010000000
VERSION     DBNHKE010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 28233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1293809"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..315
                     /locus_tag="AC16O9_09505"
     CDS             148..315
                     /locus_tag="AC16O9_09505"
                     /inference="COORDINATES: protein motif:HMM:NF037521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HIR6516891.1"
                     /translation="MQRGRKSLSFEHSGFAAVYSPEQDRTQLPEPELFAEGGGYYATA
                     LHELIQGTSRR"
     gene            379..2904
                     /gene="mutS"
                     /locus_tag="AC16O9_09510"
     CDS             379..2904
                     /gene="mutS"
                     /locus_tag="AC16O9_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR6516892.1"
                     /translation="MNSPDLHTPMMQQYLRIKAEHPDILLFYRMGDFYELFYDDAERA
                     SRLLDLTLTARGQSAGAPVTMAGVPAHSVEQYLARLVKLGESVAICEQVGDVATAKGP
                     VERQVTRIVTPGTLTDSELLDDKADNVLLALTADKATVGLAWLSLASGTLRVAEVAPQ
                     ALANELRRIGPAEILAAEGTEPEGYFITRLPAWQFDVDSGRKRLLEQLGAASLTGYGV
                     DDLGLAIGACGALLDYAKKTQGQALAHVNAVSAERTAEYLRMDAATRRNLEITETLRG
                     EPAPTLLSLLDRCASGMGSRRLHHWLHHPLRDRAAVAERHAAVERLHGSTEPIHSTLR
                     RCADVERITARIALKNARPRDLSGLRDSVGLLPGLRQAVPGESALLELLMADLETPQA
                     CLDLLCRAVAPEPAAQVRDGGVIAEGYSAELDELRRLQSGAGQFLVELEARERARTGI
                     PNLRVAYNHVHGYYIEVTHSHAEKIPAEYRRRQTLKNAERYITPELKAFEDKALSARD
                     RALALEKSLYDELLEKLGAHLAELQRIARALAQLDVLAAFAAVAQERGYCRPQFVDDV
                     RVDIEAGRHPVVEGQVERFIANDARLAPSRQLLLITGPNMGGKSTYMRQVALIVLLAH
                     AGSFVPAKAARLGPIDQIFTRIGAADDLAGGRSTFMVEMTESANILHNATAQSLVLMD
                     EVGRGTSTFDGLALAWAIARQLLERNKAPTLFATHYFEMTRIALEYREAANVHLDAVE
                     HKDSIVFLHAVEEGPASQSYGLQVAQLAGVPKAVIQRARRILQQLEEASRAGGAQADL
                     FARPAAEEPEPASDALRDALAQVNPDELSPREALELLYRLKRL"
     gene            2910..3686
                     /locus_tag="AC16O9_09515"
     CDS             2910..3686
                     /locus_tag="AC16O9_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6516893.1"
                     /translation="MPLVDIGANLTHTSFRDDLGAVLARAREHGVAQIVVTGTTVEES
                     RRAAALAEAHGLHATAGVHPHHARECDGTTIPALREIAAHPRVVAIGECGLDFNRNYS
                     PHPSQEKWFVSQLELAAELGKPLFLHSRDAHPRFAEILRNLRIGKAVAHCFTGEAAEL
                     RAYLDLGLYIGITGWICDERRGRHLVELVKDIPQDRLLLETDSPYLTPRDLHPQPKAR
                     RNEPAHLPHILRAVARARGEAPEALAEATTRNARAFFGLA"
     gene            3683..5146
                     /locus_tag="AC16O9_09520"
     CDS             3683..5146
                     /locus_tag="AC16O9_09520"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6516894.1"
                     /translation="MNLAQLLQASARRVPQAPALARGARTVASYADFAARVARLAGAL
                     RRRGLAPGERVALAMKNCPEYYEVLFACWHAGLTAVPINAKLHAREFAYILENSGARL
                     SFVTPDVESAIPGGLSVASPEFAKLRAAEPVPAADTGPDDVAWLFYTSGTTGVPKGAM
                     LTHRNLLYASQAYYADIDRLGPGDSILHAAPMSHGSGVYGIPHVAAGALNVIPESGGF
                     EPDEIFSLIAAHRGVSFFAAPTMIVRLMASPAAGGADLRHLKTITYGGAPMYVADCLR
                     AIELFGPRLYQLFGQGEAPMTITGLPQSDHATRHKLESCGFARTGVEVRVVDDADREL
                     PVGEVGEIVTRSDCVMAGYWQNAEATAKSLRGGWLHTGDIGSLDVSGYLTIRDRSKDM
                     IISGGSNIYPREIEEVLLRHPAVAECSVVGRSHAEWGEEVVAFVVRRADVSAQELDAL
                     CLEHIARFKRPRDYRFVDALPKNNYGKVLKTALRELA"
     gene            complement(5143..5634)
                     /gene="bamE"
                     /locus_tag="AC16O9_09525"
     CDS             complement(5143..5634)
                     /gene="bamE"
                     /locus_tag="AC16O9_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE
                     domain-containing protein"
                     /protein_id="HIR6516895.1"
                     /translation="MMRISSGFAALLLAGCAAIDGHTLVPGKSSATEVKALMGVPALE
                     LKHADGGTLQYFERYRAGPAVYVARIGPDGVLRGLDQRLTYANIHSVRVGMGAAEVRE
                     LLGPPISVMRLPRLQRDVWEYPWRHAVRELRVLFVQFSDDGVVREVIERHDHERDPEN
                     DWG"
     gene            complement(5631..6050)
                     /locus_tag="AC16O9_09530"
     CDS             complement(5631..6050)
                     /locus_tag="AC16O9_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR6516896.1"
                     /translation="MSAARLRVARAFGPPVEGVALLSEEGFSPRYDLDRWSGVITKAG
                     HKLEGQNIKDKVCFFPTAKGGIAAGWAFYDLQWKKIAPKAFIFGVTNPVMVQGAVFAG
                     ITITEGWTPHPREVLKTGDWVRVDPLGRSIEVLRSKP"
     gene            complement(6047..7288)
                     /locus_tag="AC16O9_09535"
     CDS             complement(6047..7288)
                     /locus_tag="AC16O9_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_277762492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR6516897.1"
                     /translation="MQLTGEERALLGGERGAAARRALEYQIKVGEFWGAKRFVPVTNV
                     HMMGDIEVMGDGGLQWLKDTAALGARCAVSTTTNARCVDFAHCERLGQDAGEVAKERE
                     LIATLRRMDVVTTDTCINYQTVYQPHLGEHVAWGDTGTVIYANSVFGARSNFESGPAA
                     LAAAITGRTPEYGFHLDAHRRGVFTVELRARLDDLADWGAVGKLVGEPHQSYFSVPVF
                     HGFHRTPGADELKHLGAALASYGSMGMFHFVGATPEAPTLEAALGGRAPAERLAVTDA
                     DLERVYAGYGLGDGDARLVVFSGPQQSLLEMKQLAALFDGRRVHPGGHCIVTTNGGVL
                     AQSRALGYAKALEDAGVTILEGVCFYILQNLSPMRVANKWSNMVSNSAKIVNIIGAHR
                     FNTILRRTRECVDIACTGVLR"
     gene            complement(7289..7627)
                     /gene="fdxA"
                     /locus_tag="AC16O9_09540"
     CDS             complement(7289..7627)
                     /gene="fdxA"
                     /locus_tag="AC16O9_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR6516898.1"
                     /translation="MTYVVTEDCIKCKHMDCIEVCPVDCFYEGENMVVIHPDECIDCG
                     VCEPECPPKAILPDSDPKAERWLQMNTEFSLKWPNITRKGTPPPDADEWKGVPDKYAK
                     YFTPNPGKGE"
     gene            complement(7673..8236)
                     /locus_tag="AC16O9_09545"
     CDS             complement(7673..8236)
                     /locus_tag="AC16O9_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR6516899.1"
                     /translation="MRTHTRRRFIGAAVALPFVGAAFARDLPLTPACGPQASLTPRQS
                     EGPYYTPNTPLRASLVEPGTKAERLLLTGRVVSPQCRPVSRALLDFWHCDENGEYDNV
                     GFRYRGHLFADAEGRFRLETIFPAPYPGRARHIHVKVQAPGGRILTTQLYFPGDARDG
                     LWRPELVVAMAQRGESKTAAFQFIVDA"
     gene            complement(8241..9437)
                     /locus_tag="AC16O9_09550"
     CDS             complement(8241..9437)
                     /locus_tag="AC16O9_09550"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR6516900.1"
                     /translation="MPVRQEIREGRVPVKVYTDLVEEAARRQLVNISQLPIVHHHVAA
                     MPDVHHGIGATVGSVIPTLRAIIPAAVGVDIGCGMIAARLSLTANEIDEASLKKVFAQ
                     ISRDVPVGFEQHDERDARSDAAKPFKRGLRAILDKHPGVEKRVGKNSSWVRQLGTLGG
                     GNHFIEVCLDEAQRVWVMLHSGSRGIGNAIGTYFIALARQDAERHQLVLPDRDLAYFS
                     EGAQHFEDYVAAVAWAQDYARANRAEMMDLVLAALRRHLPAFEVTTEAVNCHHNYVER
                     ETHYGESVWVTRKGAIRARAGDLGIIPGSMGTRSYIVRGKGGAEAFQSCAHGAGRRMS
                     RTQAEKRYSVADLAEQTAGVVCRKDKGVLDEIPGAYKDIDEVMANQSDLVEVLHTLKQ
                     IVCVKG"
     gene            complement(9447..9860)
                     /locus_tag="AC16O9_09555"
     CDS             complement(9447..9860)
                     /locus_tag="AC16O9_09555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6516901.1"
                     /translation="MGRRFTTERKIRFSHCDPAGIVYFVNFFDMVNGVVEDWFGEAIG
                     FTFNEMHIQRRVGFPIVNTGCEFFRPCHLGDLLVLELSIAKLGRSSIEFAVLGRVAGD
                     EKFRARHKVVLMSLESQRALPIPTDLREKMQPFVV"
     gene            complement(9864..11048)
                     /locus_tag="AC16O9_09560"
     CDS             complement(9864..11048)
                     /locus_tag="AC16O9_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HIR6516902.1"
                     /translation="MTNRTQVAIIGAGPAGLLLAHLLRLDDIESVVLETRTREEIEAT
                     IRAGVLEQGTVNILNETGVGERMMHEGAVHHGIYLRFDGRTRRIDLAGLTGGRSIMVY
                     AQHEVIRDLVKARLDAGAPIEFGAKDVTLQDLDTDAPRVRFRNAAGELCELACDYVAG
                     CDGFHGVSRPSMPSRRRHEFSRSYPFGWLGVLVQAPPSTDELIYAHHERGFALVSTRS
                     PEIQRLYVQCDPEDRIENWPDDRVWEELRARLATADGWVLKEGRIFQKGIVAMRSFVV
                     EPMQYGRLFLAGDAAHIVPPTGAKGLNLAVADVRVLARALKAHYQDHRNDLLAQYSAT
                     ALRRTWRVEHFSWWMTSMLHRFHDDTPFQHRLQLAELDYLTSSRAKATTLAENYVGIP
                     LD"
     gene            11130..11846
                     /locus_tag="AC16O9_09565"
     CDS             11130..11846
                     /locus_tag="AC16O9_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516903.1"
                     /translation="MRILIISLLSALLAAAPAAAQEIDQLQTIGQSQFRLLSEDLGAA
                     LSYHAQTPAEPLGITGFDFGVGVTATKMENAAILQEVTSDDAETTIYVPTIRLHKGLP
                     AGFDIGLTYASVPGSNIRYTGGELRYAILQGGVASPALAVRGSLTKLSGVDQLSLDTR
                     GVDISISKGFAFLTPYAGIGRVWVESKPSGVPLLVAEKFTLTKAFIGIGMNFALLNVN
                     FQVDKTGDATSSSVKLGWRF"
     gene            complement(11843..12700)
                     /locus_tag="AC16O9_09570"
     CDS             complement(11843..12700)
                     /locus_tag="AC16O9_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6516904.1"
                     /translation="MNIENFVAIDMHTHAWKSALRVGDTPTESQQAMGNYFRYTPQHQ
                     TVPEMADMYRKLKMAFVVFTVDGEKGASRGITNEEVAELAHSNADVAIPFASINPHRG
                     AEGVKMARRLIRDYQVRGFKFHPSVQEFYPNDRMAYPLYEVIAEAKLPALFHTGQTGV
                     GAQTPGGGGIRLKYSNPMLLDDVAADFPAMPLILAHPSFPWQEEALSVATHKPQVYID
                     LSGWSPKYFPPLLVQYANTLLKDRILFGSDYPVIAPEKWLAEFDKLPIKPAVRPLILK
                     QNAARLLGL"
     gene            complement(12697..14445)
                     /locus_tag="AC16O9_09575"
     CDS             complement(12697..14445)
                     /locus_tag="AC16O9_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HIR6516905.1"
                     /translation="MQRETAWRRVPLGAADVAVERRRDGAILLRSPHPLGPYPRNLIG
                     RLAHWARERPGHVLAAERDAHGGWRTLSYGEAFRRARRVGQALLERRLSAERPLAILS
                     ENSLEHLMLMLGALYAGVPCAPVSPAYSLVSTDHAKLRHVMALATPGLVFAQDEARYA
                     GAVGASVPKGAEVVFATVPEAEPTAAVDDAYGRTGGDTIAKLLFTSGSTGQPKAVIQT
                     QRMWCANQQMLAQALPFLAAVPPVLVDWLPWHHTAGGSHNIGIVVYNGGTLHIDEGRP
                     VPGMIEKTVANLRDVAPTIYFNVPRGYEALLPFLRADEALRRSFFSRMGLMQYAAAVL
                     PQAVWQAYEELAERSCGERILWITGYGATETAPFAMSTNRGAARAGTVGLPVDGLEMK
                     LAPVNGKLEARFRGPSITPGYWRQPALTRAAFDEEGYYRTGDALRFLDPGDPAQGLEF
                     DGRIAEDFKLTTGTWVSVGRLRAEVIAAGNPWVQDAVIAGHDRDEVCALVIPRPGAKE
                     PDLQKLLDALAARSTGSSTRIARAALLAGPLSIDAGEITDKGSINQRAVLQRRADLVE
                     MLYTEPYGPQVLKANT"
     gene            14506..15720
                     /locus_tag="AC16O9_09580"
     CDS             14506..15720
                     /locus_tag="AC16O9_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516906.1"
                     /translation="MSFDRRRFNQTLAAATLGSLAPFNIARAQSGRLKVGVLLPRSGI
                     QAGIGQDCHRVVEITNGLLRDLGLPALDIMNGDTESNVDVARSRAERLIGEGAQLLVG
                     AFDSGQTTAIAQVAEQRGIPLVINIAAAPPITEQGYKFVFRNFATAPMILGDAFANQL
                     EVFAATGVSPKSVVFMHVNDTFGQAMQRGFTGVMKKFTMPYTVADTIAYDPAARDLSV
                     EVAKAKATGAEALVMVSRLNDAILITRELVKQRWNPTAVLSMGPGWYEDQYLKTLGKL
                     GDGPISFVPWYDPNKKLAQQLQAAHQKAYPDISLNTNHVYTFEALLVAADAHKRAGST
                     DPKALADAIRATNITNNVSPGPGIQFDAKGQNSKLKNSAIQNRGGKLVTVAPKDAADA
                     KPQLPMTPYDKR"
     gene            15745..16620
                     /locus_tag="AC16O9_09585"
     CDS             15745..16620
                     /locus_tag="AC16O9_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516907.1"
                     /translation="MPVEIYLNVAASGLLTGLVYGLMALGLSVIFGVVRVVNFAHGEM
                     MTLAMYLTLVLFSALKLDPLILLVPVAAVLFVFGYLLQRGLINPFINRPEHSQFMLLV
                     AVALVLVNVQLIVFGPDAQGIRTAYSFDSFQLGALIVDASKVYAAGAALVVAGALFAF
                     FRWTLLGKAIRACADNYDGARVVGLDVKRLYALTFGIGSACVGAAGAMLMAVIDVTPT
                     IGPAYTLLAFVIVITGGLGSMPGALLGGLLIGLTEALGGVLFTPSAKSMFAFGLLVLV
                     LLFRPQGILGRKAAA"
     gene            16617..17615
                     /locus_tag="AC16O9_09590"
     CDS             16617..17615
                     /locus_tag="AC16O9_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516908.1"
                     /translation="MRHLLDDVPRRGLWFLAALLALLLVFPFFASGYLLAVGFLILYF
                     AYVGQAWNVMMGFAGQLSLGHSLYVGLGAYAAAALYVHFGVSPWVGLGASIAVCALAG
                     AVIGFLAFRFGVAGVYFAILTIAFAEFARIGFDHFAWVRGSSGLFLPVANYTENDLWN
                     LRGSPTMFYYLMLALTVAAFVLCHALLRSRMGYYWLAIRESPEAAQALGVNLFRYKMA
                     AVVLSAAMTSLAGVVFAFYYNNLFPEQIFHISRSIELILGPIIGGIGTLAGPIVGAFL
                     LTGLAEGLRELMLQLGIEVPGVKQVFYGLCLLLVVMFLPHGVWPPLARRLGLERKR"
     gene            17617..18336
                     /locus_tag="AC16O9_09595"
     CDS             17617..18336
                     /locus_tag="AC16O9_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516909.1"
                     /translation="MLRIEGLTKSFRGLRAVHDASFAVPEGAIVGLIGPNGAGKTTCF
                     NMIAGVFRPDAGQVRFAGRRIDGWRPDQVCAAGIGRTFQIVKPFAGLSVIDNVVVGAL
                     LKSKDVSAAKSYAAEILERLGLGAKRGLPASSLTLPDRKRLEVARALATRPKLLLLDE
                     VMAGLRPTECDQMVEVFRELNRAEGLTILLIEHVMRAVMALAQTIVVLHHGEVIARGA
                     PQDVVRDPAVLECYLGEETPV"
     gene            18336..19040
                     /locus_tag="AC16O9_09600"
     CDS             18336..19040
                     /locus_tag="AC16O9_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516910.1"
                     /translation="MLAVENLDLYYGDAQALAGVSLEVPQGEIVAIVGANGAGKSSLI
                     RTIAGIERARAGRITFKGADLTRLESHRICNLGIGQVAEGRQVFPSLTVEENLQMGAL
                     LPRARAGAARALEEAYAMFPRLAERRTQLAGTLSGGEQQMLAIGRCLMGQPELVMFDE
                     PSLGLAPLVVQEVLHTIRALNARGLTVLLVEQNVAVSLKLSSRAYVLENGSIVMRGTG
                     AELLRDDRVRQAYLGL"
     gene            19050..19868
                     /gene="hslO"
                     /locus_tag="AC16O9_09605"
     CDS             19050..19868
                     /gene="hslO"
                     /locus_tag="AC16O9_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR6516911.1"
                     /translation="MQTDYVQRFLFERLAIRGRLVCLTGAWTRMLEGRGYPARIAETL
                     GHGTALAVLLGAGQKGAGRVTLQAQGSGPLKLLVADCTSELKVRGMAHYAGEAPELGD
                     GRLAVSYEDDASGRIYQSLVPLEGATLAEVFENYLRRSEQREAFLHLRASDAALCGLL
                     LEKLPGADEKDADGWNRVTTLAATLTLEETVDAQPYDLLVKLFHEELLRVFRLYPVEY
                     HCPWNPASVERVLRSLGRPEVESILAEKGEVVVKNEMCNHEYRFDRAAVEAVFA"
     gene            complement(19869..20984)
                     /locus_tag="AC16O9_09610"
     CDS             complement(19869..20984)
                     /locus_tag="AC16O9_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HIR6516912.1"
                     /translation="MTAKLFTPIQLGGITLPNRIVVSPMCQYSAADGAMTDWHTVHLG
                     SLACSGAGLTIIEATGVTREARITHGCTGLYTDQNEAAMKRVVEVFRGISKAPIGVQL
                     AHAGRKASTQVPWLGGKPLPAAESPWQTVGPSPVPFAEGWHVPHELTVEEIRGLADAF
                     AAAAQRARRIGLDLIELHSAHGYLLHQFLSPLSNKRSDRYGGSLENRLRAPLEIARAL
                     REAWPREKALGARITASDWAPGGFEVDDAAVYARELKAIGLDYVCVSSGGEAAHQKIK
                     VEPGYQVGFAEQVKRKVQMNVMAVGMIADPQQAEDILAAGRADMVALARGLLDDPRWV
                     WHAAERLGVKVEFPPQYRRARFDLWPGAELARPKQAA"
     gene            complement(20984..21613)
                     /locus_tag="AC16O9_09615"
     CDS             complement(20984..21613)
                     /locus_tag="AC16O9_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR6516913.1"
                     /translation="MKQETGRYEPYNYFQLMEVSLRELLVAKGIVTEDAIAGAMAVMR
                     ARGPERGAAMVARAWADPAYKARLLENGSRAAEELGFEVPGLRLIVVENTPETHNAIV
                     CTLCSCYPRILLGLPPEWYKSKPYRSRMVREPRKVLAEFGLKVPAKVQIRVHDSNADM
                     RYMVLPMRPAATGGWDEARLAGLVTRDCLIGVAVPTLPKIPKKPKPRKS"
     gene            complement(21610..21894)
                     /locus_tag="AC16O9_09620"
     CDS             complement(21610..21894)
                     /locus_tag="AC16O9_09620"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HIR6516914.1"
                     /translation="MPDARYRAGDKVRVRKAAPLGHVRTPYYIRGHSGVVERVCGAFP
                     NPEELAQQRDGLPAQPLYRVRFRQSDVWPGYRGSRDDVIELEIFQHWIEP"
     gene            complement(21881..22201)
                     /locus_tag="AC16O9_09625"
     CDS             complement(21881..22201)
                     /locus_tag="AC16O9_09625"
                     /inference="COORDINATES: protein motif:HMM:NF045268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516915.1"
                     /translation="MIERSHHDMGGLPAGKVRRTEHAYDDWELRVDAMMMLLVGVLGG
                     ERRMTVDELRKNIESLPPADYDKMGYYDRWVISLTQTMIQRGVFTTDELARKLAEVEK
                     RARR"
     gene            complement(22211..23143)
                     /locus_tag="AC16O9_09630"
     CDS             complement(22211..23143)
                     /locus_tag="AC16O9_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR6516916.1"
                     /translation="MAQHRLDPSVENVHWGYFDAKLPPRLEVASGDTVVVDTVSGWAD
                     VAPGETRFSPAHQEIITRLKPQLGPHILTGPIAVKGAMPGDTLEVKIRAIELTADWGW
                     NVIRPLRGTLPEDFPEFQARVIPIDRNKMTAKLAWGPTIPLQPFFGILATAPRAEYGR
                     ISTVEPREFGGNVDCKEYVPGTSLFLPVFVPGANFSAGDGHAVQGDGEVCLTALETCL
                     KGTFELVLHKHMPLAMPRAVTPTHYITIGLNEDLDDAAKQALRGMIAWLVEMKGWKPA
                     EAYVFCSLAADLHVTQLVDGNKGVHAMVRRDLLQ"
     gene            complement(23155..23883)
                     /locus_tag="AC16O9_09635"
     CDS             complement(23155..23883)
                     /locus_tag="AC16O9_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516917.1"
                     /translation="MAESLLSVRGIHTYYGDSYVLQGLSLELREGTIVAILGRNGMGK
                     TTLIRSVAGLTPPREGEIRFKGASLAGRPPYAIAQAGIALVPQGRRIFRSLSVRENLL
                     LPTSGLARARPGRWNLPAVLEQFPQLAERIQHSGGALSGGEQQMLAVGRALMANPDLI
                     LMDEPSEGLAPKLVQQVGSIMQDVRAHGHAVLLVEQNFALAMSVADYIYVVSAGRFVF
                     EGTPAALEKAPEVLEQHLGIARAH"
     gene            complement(23876..24637)
                     /locus_tag="AC16O9_09640"
     CDS             complement(23876..24637)
                     /locus_tag="AC16O9_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6516918.1"
                     /translation="MADPALRVESLAKVFGGLAAVDGVSLAVAAGERRVLIGPNGAGK
                     TTLFHCITGTLKPTSGSVALFGRDVTHDPEYRRTALGMGRTFQITNVFPDLTLGEHLA
                     LAIVGTDRRKWVLHRPAEAFDEVRARALEGLARVGLEGRAHLPAKLVSYGERRQLELA
                     LALNTNPKVLFLDEPCAGLSPAERQRISQMIAALPRGITLLMIEHDMDVALGLADRVT
                     VLHQGRVILEGSPDEVRANAQVRDVYFGHGEALRG"
     gene            complement(24630..25574)
                     /locus_tag="AC16O9_09645"
     CDS             complement(24630..25574)
                     /locus_tag="AC16O9_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516919.1"
                     /translation="MDRARLALIVVALAFAAALPWLGGPFWTGLATQMLIFGLLALSV
                     DLLLGHAGLFSLTHASFFAVSAYTVAILQVRYGWPTIAAAPAGIVLGTLLAAVFGISV
                     RTRGVYFILITIALGYVVWGVAHRWSSFTGGDNGVTNVPFPALGPAAITSHTQYYYVV
                     LAAVILCGLGYRVLIRSPFGLGLRGIKASETRMQSLGYRTGLHLYAAFVLSGALASFA
                     GVLYVYYNKFVNPVASSFPVSVEAALMAIVGGTGSIVGPFLGAAVVLGLRNWVSSFVE
                     MHHAIMGAVFIATVLWAPQGLVGIAQRIRARLAKGRHG"
     gene            complement(25577..26437)
                     /locus_tag="AC16O9_09650"
     CDS             complement(25577..26437)
                     /locus_tag="AC16O9_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6516920.1"
                     /translation="MTSAILQAINGVSLAALLFLLASGFTLSFGLMRVVNMAHGAYYL
                     LGGYIGLTIAEKTGSFALALAGGGAAIIVVGYFIDRFLIRRTGENHLAQVLLTVGIAF
                     VIGDVALQIWGGDNLMVPAPQFLKGAVVLPGDIYYPKYRFVLIFLGLAVGAALWLLYK
                     KTQLGAVVRAGVDDREQVNATGINVDRLFVMVSALASFLAGMAGVAGGAFLTLYPGAE
                     WEILVYALVVVIVGGLGSLEGAMIGALIVGLLDAYGRWLLPEFSYFVLFGPIALLMIF
                     RPRGLFGREG"
     gene            complement(26498..27733)
                     /locus_tag="AC16O9_09655"
     CDS             complement(26498..27733)
                     /locus_tag="AC16O9_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6516921.1"
                     /translation="MKSLKMNVSLGSIVALLAAIGMGTGAHAQSGPIKIGVLAPVTGP
                     LATPGKDMVDGWKMFWDQAGHSAGGRKVEYVIADTTCNPDQALTQARRLVHQEKVHFM
                     VGPLCGHEGPAVAQVSKETGVPLVMDAAGADVITKWDRSPTVVRTAVSASQIGHPWGE
                     YMYKELGLRNVTFIGQDYTWGHEVTLGAVRTFTALGGKVAKMIWNPIGTKDYGPTIAA
                     IPEDTDGVSAVVVGPDRVRLFEAWFNFGMHKKKKIYGGYWMHQDVLPQMDDRAIGMIG
                     NSLNYAAGLQTPENIAFTTEFAKRTNRLPSWFGESAYSAGVWTKAAIDKIGGKVEDRA
                     AFLNAMRTVTIKGPRGPLRMDDYGNPIQNVYISKIQKIKHPVLGEVLTNVPFKTYEGV
                     SQFWTFDPKEFLARGPYKR"
     gene            27773..>28233
                     /locus_tag="AC16O9_09660"
     CDS             27773..>28233
                     /locus_tag="AC16O9_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6516922.1"
                     /translation="MTDDHQRFGSSRGAPRSEDDPLLAGRGRFTDDLKTAQHARAAFV
                     RSPMGHATIRAVDAKAAARMPGVLAILAGADLALGAIPPGVLLPGRDGKPMFATAMPV
                     LATDRVRYVGEPVALVVAETLSQALDAADAVQVEYGELPAVSTVARALAKGA"
BASE COUNT         4230 a         9970 c         9681 g         4352 t
ORIGIN      
        1 cccggctcga tgcggaagtc gctgttattg accacaacgc cgattgtccc gccgatgctg
       61 ggcgcgccct gcatgcgcgc cggtcggacc ttcgcccctt gagccccgaa tcgcgccctc
      121 gccgccctcg gccgcgcgtg ttgcacagtg caacgcggaa ggaagtccct atccttcgag
      181 cacagcgggt tcgcggccgt ctacagtccc gagcaagacc gcacccagct ccccgagccg
      241 gagctcttcg ccgagggcgg gggctactac gcgacggcgc tccacgaatt gattcagggg
      301 acgtcccgtc ggtagacggc ccacgacgga cccgtcaata ttaggcgccc aataggcgct
      361 cccagccaag cacttggaat gaacagcccg gaccttcaca cgccgatgat gcagcagtac
      421 ctgcgcatca aagcggagca ccccgacatc ctgctcttct accggatggg ggacttctac
      481 gagctgttct acgacgacgc ggagcgcgcc tcgcgcctcc tggacctgac gctcaccgcg
      541 cgcggccagt cggcgggcgc gccggtcacc atggcgggcg tgcccgcgca ttcggtcgag
      601 cagtacctgg ccaggctggt gaagctcggg gagtccgttg cgatctgcga gcaggtcggc
      661 gacgtcgcca cggccaaagg cccggtcgag cgtcaagtga cgcgcatcgt cacccccggc
      721 acgctcaccg attcggaact gctggacgac aaggccgaca acgttctgct cgcgctgacg
      781 gcggacaagg ccaccgtggg cctcgcctgg ctgtcgctcg ccagcggcac gctgcgcgtg
      841 gcggaggtgg cgccgcaggc gctggccaac gaattgcgcc gcatcggccc ggcggagatt
      901 ctcgccgcgg aagggaccga gccggagggc tacttcatca cgcgcctgcc cgcctggcag
      961 ttcgacgtcg attccggcag gaagcgcctg ctcgagcagc tcggcgcggc gagcctcacc
     1021 ggctacggcg tggacgacct cggcctcgcc atcggcgcct gcggcgcgct gctcgactac
     1081 gcgaagaaga cccagggaca ggcgctggcg cacgtcaacg cagtgtcggc ggagcgcacc
     1141 gcggaatacc tgcgcatgga cgccgcgacg cggcgcaacc tggagattac cgagacgctg
     1201 cgcggcgagc ccgcgccgac gctgctctcg cttctggacc gctgcgcgag cggcatgggc
     1261 agccgccggc tgcaccactg gctgcaccac ccgctgcgcg atcgcgcggc agtcgcggag
     1321 cggcatgcgg ccgtggagcg gctgcacggc agcacggaac cgatccactc gacgctgcgc
     1381 cgctgcgccg acgtcgagcg catcaccgcg cgcattgcac tgaagaacgc gcggccgcgg
     1441 gacctttcgg gattgcggga cagcgtcggg ctgctgcccg ggctgcggca ggcggtaccc
     1501 ggcgaaagcg cgcttctcga gctgctgatg gccgatctgg agacgccgca ggcttgcctc
     1561 gatctcctgt gccgcgcggt cgcaccggag cccgcggcac aagtgcgcga cggcggcgtg
     1621 atcgccgagg gctacagcgc cgaactcgac gagctgcgcc gactgcagtc cggcgccggc
     1681 cagttcctgg tggagctgga agcgcgcgag cgcgcgcgca ccggcatccc caacctgcgc
     1741 gtggcctaca accacgtgca cggctactac atcgaggtga cgcactcgca cgccgagaag
     1801 atccccgccg agtaccgccg ccggcagacc ctgaagaacg ccgagcgcta catcacgccc
     1861 gagctgaagg cgttcgagga caaggcgctc tcggcgcgcg atcgcgcgct ggcgctggaa
     1921 aaatcgctct acgatgaact gctcgaaaag ctgggcgcgc accttgccga gctgcagcgc
     1981 attgcgcgcg ccctcgcgca gctcgacgtg ctcgccgcct tcgccgcggt ggcgcaggaa
     2041 cgcggctact gccggccgca attcgtggac gacgtgcgcg tcgacatcga ggccgggcgc
     2101 cacccggtgg tggaaggcca agtcgagcgc ttcatcgcca acgacgcgcg ccttgcgccg
     2161 tcgcgccagc tgctgctcat caccgggccg aacatgggcg gcaagtccac ctacatgcgc
     2221 caggtggcgc tgatcgtgct gcttgcgcac gccggatcct tcgtgcccgc gaaggccgcg
     2281 cgcctcggcc cgatcgacca gatcttcacg cgcatcggcg ccgccgacga cctcgccggc
     2341 gggcgctcca ccttcatggt ggagatgacc gagagcgcca atatcctgca caacgccacg
     2401 gcgcagagcc tggtgctgat ggacgaggtc ggccgcggta cttcgacctt cgacggcctg
     2461 gcgctcgcct gggccatcgc gcgccagctg ctggagcgca acaaggcgcc gacgctcttc
     2521 gccacccatt acttcgagat gacgcgcatc gcgctcgagt acagggaggc ggccaacgtg
     2581 cacctcgatg ccgtggagca caaggactcg atcgtcttcc tgcacgccgt cgaggaaggt
     2641 cccgcctcgc aaagctacgg cttgcaggtg gcgcagctcg ccggggtgcc gaaggccgtg
     2701 atccagcgcg cgcggcgcat cctgcagcag ctggaagagg cgagccgcgc cggcggggcg
     2761 caggccgatc tcttcgcccg gcccgccgcc gaggagcccg agccggcgag cgatgcgctg
     2821 cgcgacgcgc tggcgcaggt caatcccgac gagctctcgc cgcgcgaggc gctggagctg
     2881 ctgtaccggc tgaagcggct gtaggcgcca tgccgctcgt ggacatcggg gcgaacctca
     2941 cgcacacgtc gtttcgcgac gacctcggcg cggtgctggc gcgcgcgcgc gagcatgggg
     3001 tcgcgcagat cgtcgtcacc ggcaccacgg tggaggaaag ccgccgcgcc gcggcgctgg
     3061 ccgaggcgca cggcctgcac gccaccgcgg gcgtgcaccc gcaccacgca cgcgagtgcg
     3121 acggcaccac gatacccgcg ctgcgcgaga tcgcggcgca cccgagggtc gtggcgatcg
     3181 gcgagtgcgg gctggacttc aaccgcaact actcgccgca ccccagccag gagaaatggt
     3241 tcgtctcgca gctcgagctc gccgccgagc tcggcaagcc gctgttcctg cactcgcgcg
     3301 acgcacaccc gcgcttcgcc gagatcctca ggaacctgcg catcggcaag gcggtggcgc
     3361 attgcttcac cggcgaagcg gccgagctgc gcgcctacct cgacctgggc ctgtacatcg
     3421 gcatcacggg atggatctgc gacgagcgcc gcggccggca cctggtcgag ctggtgaagg
     3481 acatcccgca ggaccgcctg ctgctcgaga ccgactcgcc ctacctcacg ccgcgcgacc
     3541 tgcatccgca gcccaaggcg cgccgcaacg agccggcgca cctgccccac atcctgcgcg
     3601 ccgtggcgcg cgcgcgcggc gaagcgcccg aggcgctcgc cgaggcgacc acgcgcaacg
     3661 cccgcgcttt cttcggactc gcatgaacct cgcgcaattg ctacaagcga gcgcgcgccg
     3721 cgtgccgcag gcgcccgccc tggcgcgcgg cgcgcgcacc gtggcgagct acgccgactt
     3781 tgccgcgcgc gtggcgcggc tcgccggcgc cctgcgccgc cgtggtcttg cgcccggcga
     3841 gcgcgtcgcg ctcgccatga agaactgccc cgaatactac gaggtgctgt tcgcctgctg
     3901 gcacgcgggc ctcacggccg tgccgatcaa cgccaagctg cacgcgcgcg agttcgccta
     3961 catcctggag aactccggcg cccgcctcag cttcgtcacg cccgatgtcg agtccgcgat
     4021 ccccggcggc ctgtcggtcg cctcgccgga gttcgccaag ctgcgcgcgg ccgagccggt
     4081 tcccgctgcc gacaccggcc ccgacgacgt cgcctggctg ttctacacca gcggcaccac
     4141 cggcgtgccc aagggcgcga tgctgacgca tcgcaacctg ctctacgcca gccaggcgta
     4201 ctacgccgac atcgaccgcc tcggccccgg cgactcgatc ctgcacgccg cgcccatgtc
     4261 gcacggctcg ggcgtgtacg gcatcccgca cgtcgccgcc ggcgccctca acgtgatccc
     4321 ggaaagcggc ggcttcgagc ccgacgagat cttcagcctc atcgccgcgc accgcggcgt
     4381 ctccttcttc gccgcgccga ccatgatcgt gcgcctgatg gcgagccccg cggcgggcgg
     4441 cgccgacctg cgccatctga agacgatcac ctacggcggc gcgccgatgt acgtcgccga
     4501 ctgcctgcgc gccatcgagc tgttcggccc gcgcctgtac cagctgttcg gccagggcga
     4561 ggcgccgatg accattaccg ggctgccgca atcggatcac gccacgcgcc acaagctgga
     4621 gtcctgcggc ttcgcccgca ccggcgtcga ggtgcgcgtg gtggacgacg cggaccgcga
     4681 gctccccgtg ggcgaggtcg gcgagatcgt gacgcgctcg gactgcgtga tggcgggcta
     4741 ctggcagaac gccgaggcca ccgcgaagtc cctgcgcggc ggctggctgc acacgggcga
     4801 catcggcagc ctcgacgtct cgggctacct cacgatccgc gaccgctcca aggacatgat
     4861 catctcgggc ggctcgaaca tctacccgcg cgagatcgag gaagtgctgc tgcgccaccc
     4921 cgcggtcgcc gagtgctcgg tggtggggcg ctcgcacgcc gaatggggcg aggaagtggt
     4981 ggcgttcgtc gtgcggcgcg ccgacgtgtc ggcgcaggag ctggacgcgc tctgcctcga
     5041 gcacatcgcg cgcttcaagc gcccgcgcga ctaccgcttc gtggatgcgc tgcccaagaa
     5101 caactacggc aaagtgctca aaaccgccct gcgcgagctc gcctagcccc agtcgttttc
     5161 cggatcgcgc tcgtggtcgt gccgctcgat gacctcgcgc acgacgccgt cgtcggagaa
     5221 ctgcacgaac agcacgcgca actcccggac ggcatgccgc caggggtact cccagacgtc
     5281 gcgctgtagg cgcggcagcc gcatgacgct gatcggcggc ccaagcagct cgcggacttc
     5341 cgcggcgccc atgccgacgc ggacactgtg gatgttcgcg taggtcagcc gctggtcgag
     5401 gccgcgcagc acgccgtccg gtccgatgcg cgcgacgtaa acggccggcc cggcacggta
     5461 acgctcgaaa tactggagcg tcccgccatc cgcgtgcttc agctcgagcg cgggcacgcc
     5521 catcagcgcc ttcacctcgg tcgcgctcga ctttccggga accagcgtat ggccgtcgat
     5581 ggcggcgcag cccgcaagga gcagcgccgc aaacccgctc gatatgcgca tcatggcttg
     5641 ctcctcagca cctcgatcga tcgtcccagg ggatccaccc gcacccagtc gccggtcttc
     5701 agcacctcac gcgggtgggg cgtccaccct tccgtaatgg tgattccggc gaacacggcg
     5761 ccctgcacca tcaccgggtt ggtcaccccg aagatgaacg ctttgggcgc gatcttcttc
     5821 cactgcaggt cgtaaaacgc ccaccctgct gcgatgcccc ctttggcggt ggggaagaag
     5881 cacaccttgt ccttgatgtt ctggccctcg agcttgtggc cggccttggt gatgacgccg
     5941 ctccagcggt ccaggtcgta gcgcggcgag aacccctcct cggacagcag cgccacgccc
     6001 tccaccggcg ggccgaaggc gcgcgccacg cgcaggcgcg ccgcgctcac cgcagcactc
     6061 ccgtgcacgc gatgtcgacg cactcgcgtg tgcgccgcaa gatggtgttg aagcggtgcg
     6121 cgccgatgat gttcacgatc ttcgcggagt tcgacaccat gttggaccac ttgttcgcca
     6181 cgcgcatcgg cgacaggttc tgcaggatgt agaagcacac gccctcgagg atcgtgacgc
     6241 cggcgtcctc cagggccttg gcgtagccca gggcgcggga ctgcgccagc acgccgccgt
     6301 tggtggtcac gatgcagtgc ccgcccgggt gcaccctgcg cccgtcgaag agggcggcga
     6361 gctgcttcat ctcgagcagc gactgctgcg ggccggagaa cacgaccagg cgcgcgtcgc
     6421 cgtcgcccag cccgtagccg gcgtagacgc gctcgaggtc cgcatccgtg accgcgaggc
     6481 gctcggccgg cgcgcggccg ccgagcgcgg cctccagcgt gggcgcttcg ggcgtcgcgc
     6541 cgacgaaatg gaacatgccc atcgagccgt agctcgcgag cgccgcgccc aggtgcttca
     6601 gctcgtcggc gcccggcgtg cggtgaaagc cgtggaacac cggcaccgag aagtagctct
     6661 ggtgcggctc gcccaccagc ttgccgaccg cgccccagtc ggcgaggtcg tccaggcgcg
     6721 cgcgcagctc gacggtgaag acgccgcgcc gatgcgcgtc gagatgaaag ccatactcgg
     6781 gcgtgcggcc cgtgatcgcc gcggcgagcg cggccgggcc cgactcgaag ttgctgcgcg
     6841 cgccgaacac cgaattggcg tagatcaccg tgcctgtgtc gccccaggcc acgtgctcgc
     6901 cgaggtgcgg ctggtacacc gtctggtagt tgatgcaggt atcggtggtc accacgtcca
     6961 tgcgccgcag cgtggcgatc agctcgcgct ccttggccac ttcgcccgcg tcctgaccga
     7021 ggcgctcgca gtgcgcgaaa tcgacgcagc gcgcgttggt ggtggtgctg acggcgcagc
     7081 gcgcgccgag cgcggcggtg tccttcagcc actgcaggcc gccgtcgccc atcacctcga
     7141 tgtcgcccat catgtggacg ttggtgaccg gcacgaagcg cttggcgccc cagaactcgc
     7201 ccaccttgat ctggtactcg agcgcgcggc gcgcggcggc gccgcgctca ccgccaagga
     7261 gggcgcgctc ctccccggtc agctgcacct actccccctt gccggggttc ggggtgaagt
     7321 atttcgcgta cttgtcgggg acgcccttcc actcgtccgc gtcgggcggc ggcgtgccct
     7381 tgcgcgtgat gttgggccac ttcagcgaga actcggtgtt catctgcagc cagcgctcgg
     7441 ccttggggtc ggagtccggc aggatggcct tcggcgggca ctcgggctcg caaacgccgc
     7501 agtcgatgca ctcgtcgggg tggatgacca ccatgttctc gccctcgtag aagcagtcca
     7561 cggggcagac ctcgatgcag tccatgtgct tgcacttgat gcagtcttcg gtgacgacgt
     7621 aggtcattgc gggcgcgcgg ggcgaattgc ggaaggcgcg attatgtcat cttcaggcgt
     7681 cgacgatgaa ctggaacgcc gccgttttgc tttcgcccct ctgcgccatg gcgacgacca
     7741 gctctgggcg ccacagcccg tcgcgcgcgt cgcccggaaa gtacagctgc gtggtgagga
     7801 tgcgcccgcc cggcgcctgc accttgacgt ggatgtggcg cgcgcggccc ggatatggcg
     7861 cagggaagat ggtttccagc cggaagcgcc cttccgcgtc cgcgaacagg tggccgcggt
     7921 agcgaaagcc gacgttgtcg tactctccgt tctcgtcgca gtgccagaaa tcgagcagcg
     7981 cgcgggacac cgggcggcac tgcggcgaca ccacgcgccc ggtgagcagc agccgctcgg
     8041 ccttcgttcc gggctcgacg agcgaggccc gcagcggcgt gttcggggtg tagtaaggcc
     8101 cctcgctctg gcggggggtg agggatgcct gcgggccgca cgcgggagtc agcggcaggt
     8161 cacgggcgaa ggcggcgccg acgaagggca aggcgaccgc ggccccgatg aatcggcgcc
     8221 gtgtgtgggt tctcatgagt tcagcctttc acgcaaacga tctgcttgag cgtgtgtagg
     8281 acttcgacca ggtcgctttg gttcgccatc acctcgtcga tgtccttgta ggcgcccggg
     8341 atctcgtcga gcacgccctt gtccttgcgg cacaccaccc ccgcggtctg ctcggcgaga
     8401 tcggcgaccg agtagcgctt ctcggcctgc gtgcggctca tgcgccgccc ggccccgtgc
     8461 gcgcaggact ggaacgcctc ggcgccgccc ttgccgcgca cgatgtagct ccgggtgccc
     8521 atgcttcccg ggatgatgcc gaggtcgcct gcccgcgcgc ggatcgcccc tttgcgcgtc
     8581 acccacacgc tctcgccgta gtgcgtctcg cgctcgacgt agttgtggtg gcagttgacc
     8641 gcttcggtgg tcacctcgaa ggccggcagg tggcgcctga gcgccgcgag caccaggtcc
     8701 atcatctcgg cgcggttggc acgcgcatag tcctgcgccc aggccaccgc cgcgacgtag
     8761 tcctcgaagt gctgcgcgcc ctccgagaaa tacgccaggt cgcggtcggg cagcacgagc
     8821 tggtggcgct cggcgtcctg ccgcgccagg gcgatgaaat aggtgccgat ggcgttgccg
     8881 atgccgcggc tgcccgagtg cagcatgacc cacacgcgct gcgcttcgtc gaggcacacc
     8941 tcgatgaaat ggttgccgcc gccgagggtg ccgagctggc gcacccagct ggaattcttg
     9001 ccgacgcgct tttcgacccc ggggtgcttg tcgaggatcg cgcgcaagcc gcgcttgaac
     9061 ggctttgcgg cgtcgctgcg cgcatcgcgc tcgtcgtgct gctcgaaccc caccggcacg
     9121 tcgcgcgaga tctgcgcaaa caccttcttc agggacgcct cgtcgatctc gttcgcggtc
     9181 agcgacaggc gcgcggcgat catgccgcag ccgatgtcca cccccaccgc cgccgggatg
     9241 atggcgcgca gcgtcgggat caccgagccg acggtggcgc cgatgccgtg gtgcacgtcg
     9301 ggcatcgccg cgacgtggtg gtgcacgatc ggcagctgcg agatgttgac gagctgccgg
     9361 cgcgcggctt cctcgacgag gtcggtatag accttgacgg ggacgcggcc ttcgcggatc
     9421 tcctggcgca ccggcatggc cggatgttag accacgaacg gctgcatttt ctcccgcaga
     9481 tccgtcggga tcggcagcgc gcgctgcgac tcgagcgaca tcagcaccac cttgtgccgg
     9541 gcgcggaact tctcgtcgcc cgccacccgc ccgagcacgg cgaactcgat cgagctgcgg
     9601 ccgagcttgg caatggacag ctcgagcacc agcaggtccc ccaggtggca gggcctgaag
     9661 aactcgcagc cggtgttcac gatcggaaag cccacgcgcc gctggatgtg catctcgttg
     9721 aaggtgaagc cgatcgcctc gccgaaccag tcctcgacca ccccgttcac catgtcgaag
     9781 aagttgacga aatagacgat gcccgccggg tcgcagtgcg agaagcggat cttgcgctcg
     9841 gtggtgaagc ggcgccccat cactcagtcg agcgggatgc cgacgtagtt ctccgccagc
     9901 gtcgtcgcct tggccctgga gctggtcagg tagtcgagtt cggcgagctg caggcggtgc
     9961 tggaacggcg tgtcgtcgtg gaatcgatgc agcatcgagg tcatccacca cgagaagtgc
    10021 tcgacgcgcc aagtccggcg cagcgcggtc gccgagtact gggcgagcag gtcgttccta
    10081 tggtcctggt agtgggcctt cagcgcgcgc gccagcacgc gcacgtcggc caccgccagg
    10141 ttgagcccct tggcgccggt cggcggcacg atgtgcgccg cgtctccggc gaggaagagc
    10201 cggccgtact gcatcggctc gaccacgaag ctgcgcatgg cgacgatgcc cttctggaag
    10261 atgcggccct ccttcagcac ccagccgtcg gcggtggcga gccgcgcgcg cagctcctcc
    10321 cagacgcgat cgtcgggcca gttctcgatg cggtcctcgg gatcgcattg cacgtacagg
    10381 cgctggatct cgggcgagcg cgtgctgacc agggcgaagc cgcgctcgtg gtgcgcgtag
    10441 atcagctcgt cggtcgaggg cggcgcctgc accaggaccc cgagccaacc gaaggggtag
    10501 ctgcggctga actcgtggcg gcgccgcgac ggcatcgacg ggcgcgagac gccgtgaaag
    10561 ccgtcgcagc ccgcgacgta gtcgcacgcg agctcgcaca gctcgccagc ggcattgcgg
    10621 aaccgcaccc tgggcgcgtc ggtgtcgagg tcctgcagcg ttacgtcctt cgcgccgaac
    10681 tcgatcggcg cgccggcgtc caggcgcgcc ttcaccaggt ccctgatcac ctcgtgctgc
    10741 gcgtacacca tgatcgagcg cccgccggtg agcccggcga ggtcgatgcg ccgcgtgcgc
    10801 ccgtcgaagc gcaggtagat gccgtggtgc acggcgccct cgtgcatcat gcgctcgccg
    10861 acgccggtct cgttcaggat gttgaccgtg ccctgctcca gcaccccggc gcggatcgtg
    10921 gcctcgatct cctcgcgcgt gcgcgtctcc agaaccacgg actcgatgtc gtccagccgc
    10981 aacaggtgcg cgagcagcag ccccgcgggg ccggcgccga tgatggccac ttgtgtgcgg
    11041 ttggtcacgc cgcgtcggga ttaagatgcc atactatctg ttggcgtcgg catgcggcca
    11101 ttttatcgcc gctcaccaag gagtcgatca tgcgcatcct catcatttcg cttctctcgg
    11161 cactgctggc tgccgcgccg gcggcggcgc aggagatcga ccagctgcag acgatcggtc
    11221 aatctcaatt ccgcctgctg tccgaggacc tcggcgcggc gctcagctac cacgcgcaga
    11281 ctcccgcgga acccctcggc atcaccgggt tcgatttcgg cgtcggggtc acggccacga
    11341 agatggagaa cgccgcgatc ctgcaggagg tgacctccga cgacgcggaa accacgatct
    11401 acgtgccgac gatccgcctg cacaagggcc tgcccgccgg gttcgatatc ggactgacct
    11461 acgcttcggt tcccggaagc aacatcaggt acacgggcgg cgagctgcgc tacgccatcc
    11521 tgcagggcgg cgtggcctcg ccggcgctcg cggtgcgcgg cagcctgacc aagctgagcg
    11581 gcgtcgacca gctgtccctg gacacgcgtg gcgtggacat ttccatctcc aagggcttcg
    11641 cgttcctgac gccgtacgcg ggaatcgggc gcgtgtgggt ggagagcaag cccagcgggg
    11701 tgccgttgct ggtcgcagag aaattcaccc tcaccaaggc gttcatcggc atcggcatga
    11761 atttcgcgct gctgaacgtc aacttccagg tcgacaagac cggcgacgcg acctcctcca
    11821 gcgtgaagct cggctggcgc ttctagaggc cgagcagccg cgccgcgttc tgcttcagga
    11881 tcagcggccg caccgctggc ttgatgggca gcttgtcgaa ctcggcgagc cacttttccg
    11941 gcgcgatcac cggatagtcg gagccgaaca ggatccggtc cttcagtagc gtgttggcgt
    12001 actggacgag gagcggcggg aagtacttcg gcgaccagcc cgagaggtcg atatagacct
    12061 gcggcttgtg cgtcgccacc gacagcgcct cttcctgcca cgggaacgaa gggtgcgcga
    12121 ggatcagcgg catggcggga aaatccgccg ccacgtcgtc cagcagcatc gggttcgaat
    12181 acttcaggcg gatgccgccg ccgcccggcg tttgcgcgcc gacgcccgtc tgcccggtgt
    12241 gaaagagcgc cggcagcttc gcctccgcga tcacctcgta gagcggatag gccatgcggt
    12301 cgttgggata gaactcctgc accgacggat ggaacttgaa gccgcgcacc tggtagtccc
    12361 ggatcaggcg ccgcgccatc ttcacgccct cggcgccgcg gtgcgggttg atgctggcga
    12421 acgggatcgc gacgtcggca ttgctgtgcg cgagctccgc gacctcctca ttggtgatgc
    12481 cgcggctcgc gcccttctcg ccgtccacgg tgaagacgac gaaggccatc ttcagcttgc
    12541 ggtacatgtc ggccatctcg ggcaccgtct ggtgctgggg cgtgtagcga aagtaattgc
    12601 ccatcgcctg ctgtgactcg gtcggcgtgt cgcccacgcg cagcgccgac ttccaggcgt
    12661 gggtatgcat gtcgatggcg acgaagttct cgatattcat gtattcgctt tcagcacctg
    12721 cgggccgtag ggttccgtgt acagcatttc taccagatcg gcgcggcgtt gcagcacggc
    12781 gcgctggttg atcgagccct tgtcggtgat ctcgcccgcg tcgatcgaca acggccccgc
    12841 gaggagcgcg gcgcgcgcga tgcgggtgct gctgcccgtg ctgcgcgcgg cgagcgcatc
    12901 gagcagcttc tgcaggtccg gttctttcgc gccgggccgc ggaatgacga gcgcgcacac
    12961 ctcgtcccgg tcgtggccgg cgatgacggc atcctgcacc cacggattgc ccgcggcgat
    13021 gacctcggcg cgcaggcgcc cgacgctcac ccaggtgccg gtggtcagct tgaagtcctc
    13081 cgcaatgcgg ccgtcgaact ccagcccctg cgccgggtcg ccgggatcga ggaaccggag
    13141 cgcatcgccg gtgcggtagt agccctcctc gtcgaaggcc gcgcgcgtca gcgccggctg
    13201 gcgccagtag ccgggggtga tgctcgggcc gcgaaagcgc gcctcgagct tgccgttcac
    13261 cggcgcgagc ttcatctcca gcccgtccac cggcagtccc acggtgcccg cgcgcgcggc
    13321 gccgcggttg gtgctcatgg cgaagggcgc ggtttcggtg gcgccgtagc ccgtgatcca
    13381 gaggatgcgc tcgccgcagg agcgctcggc gagttcctcg taggcctgcc acaccgcctg
    13441 cggcagcacc gccgccgcgt actgcatgag ccccatccgg ctgaagaagc tccggcgcag
    13501 cgcttcgtcg gcgcgcagga acggcagcaa cgcctcgtag ccgcgcggca cgttgaagta
    13561 gatggtcggc gccacgtcgc gcaggttcgc caccgtcttc tcgatcatgc cgggaaccgg
    13621 ccggccctcg tcgatatgga gcgtgccgcc gttgtagacc acgatgccga tgttgtggct
    13681 gccgcccgcg gtgtggtgcc agggcagcca gtccacgagc accggcggca ccgcagcgag
    13741 gaacggcagc gcctgcgcga gcatctgctg gttcgcgcac cacatgcgct gcgtctggat
    13801 cacggccttg ggctggccgg tcgagcccga ggtgaacagg agcttggcga tcgtgtcgcc
    13861 gccggtgcgc ccgtacgcgt cgtcgaccgc ggccgtcggc tcggcctccg gaacggtcgc
    13921 gaagacaacc tctgcgccct tcggcacgga tgcgccgact gcgcccgcgt agcgcgcttc
    13981 gtcctgggcg aacacgagtc ccggcgtggc gagcgccatg acgtggcgga gcttcgcgtg
    14041 gtcggtcgac acaagcgaat acgccggcga caccggcgcg cagggcacgc ctgcgtagag
    14101 cgccccgagc atgagcatga gatgctcgag ggaattttcc gacaggatgg caagcgggcg
    14161 ctcggccgac agcctgcgct cgagcagggc ctgaccgacg cgacgagcac gcctgaacgc
    14221 ctcgccgtac gagagcgtgc gccagccgcc atgcgcgtcg cgctccgcgg cgagcacgtg
    14281 cccgggccgc tcgcgcgccc agtgcgccag gcgcccgatc aggttgcgcg gatacggtcc
    14341 gagcgggtgc ggggagcgca ggagaatagc gccatcccgc cgccgctcca ccgccacgtc
    14401 cgcggccccc agcgggaccc tgcgccatgc cgtctcacgc tgcatctcgg ggattctccc
    14461 acgcagagtt ccattagaat cgcgtcatac ccacgaggag aagccatgtc cttcgatcgc
    14521 cgcagattca accagacgct ggccgccgcc acgctcggct cgctcgcgcc gttcaacatc
    14581 gcccgcgccc agtccggcag gctcaaggtc ggcgtgctgc tgccgcgctc gggcatccag
    14641 gccggcatcg gccaggactg ccaccgcgtc gtcgagatca ccaacggctt gctgcgcgac
    14701 ctggggctcc cggcgctcga catcatgaac ggcgacaccg agtcgaacgt cgacgtcgcg
    14761 cgctcgcgcg ccgagcgcct gatcggcgag ggcgcgcagc tgctcgtggg cgccttcgac
    14821 tcgggccaga ccaccgccat cgcccaggtc gccgagcagc gcggcatccc gctggtgatc
    14881 aacatcgccg ccgcgccgcc gatcaccgag cagggctaca agttcgtgtt ccgcaacttc
    14941 gccacggcgc cgatgatcct gggcgatgcg tttgccaacc agctcgaggt attcgcggcc
    15001 accggcgtgt cgcccaagtc ggtggtgttc atgcacgtga acgacacctt cgggcaggcc
    15061 atgcagcgcg gcttcaccgg cgtgatgaag aagttcacca tgccctacac ggtcgcggac
    15121 acgatcgcct acgacccggc ggcgcgcgac ctgtcggtcg aggtggccaa ggccaaggcg
    15181 acgggcgccg aggcgttggt gatggtgagc cgcctgaacg acgccatcct catcacgcgc
    15241 gagctggtca agcagcgctg gaatccgacg gcggtgctca gcatgggccc gggctggtac
    15301 gaggaccagt acctgaagac gctcggcaag ctcggcgacg gcccgatcag cttcgtgccg
    15361 tggtacgacc cgaacaagaa gctcgcccag cagctccagg ccgcgcatca gaaggcctac
    15421 ccggacatca gcctgaacac caaccacgtg tacacgttcg aggcgctgct cgtcgccgcc
    15481 gacgcccaca agcgcgctgg cagcacggat ccgaaggcgc tcgccgacgc catccgcgcc
    15541 acgaacatca ccaacaacgt cagcccgggg cccggcatcc agttcgacgc caagggccag
    15601 aacagcaagc tgaagaactc ggcgatccag aaccgcggcg gcaagctcgt cacggtggcg
    15661 ccgaaggacg ccgccgacgc caagccccag ctgccgatga cgccttacga caagcgctag
    15721 cggcgggccg cgggcggccc gctcgtgccg gtcgagatct atctcaacgt cgccgccagc
    15781 ggcctgctga ccgggctggt ctacgggctg atggcgctcg gcctgtcggt catcttcggc
    15841 gtcgtgcgcg tcgtgaactt cgcgcacggc gagatgatga cgctggcgat gtacctgacg
    15901 ctggtgctgt tctcggcgct caagctcgac ccgctcatcc tgctcgtgcc ggtcgccgcg
    15961 gtcctgttcg tcttcggcta cctgctgcag cgggggctca tcaacccgtt catcaaccgg
    16021 cccgagcaca gccagttcat gctgctggtg gcggtggcgc tggtcctggt gaacgtgcag
    16081 ctgatcgtct tcggccccga cgcgcaaggc atccgcaccg cctactcctt cgacagcttc
    16141 cagctcggcg cgctgatcgt ggacgcttcc aaggtgtacg ccgccggcgc cgcgctggtg
    16201 gtcgccggcg ccctgttcgc cttcttccgc tggacgctcc ttggcaaggc gatccgtgcc
    16261 tgtgccgaca actacgacgg cgcgcgcgtg gtgggcctcg acgtgaagcg cctctacgcg
    16321 cttaccttcg gcatcggctc ggcctgcgtc ggcgccgcgg gcgccatgct gatggcggtg
    16381 atcgacgtca cgcccaccat cggcccggcc tacacgctgc tcgccttcgt catcgtgatc
    16441 accggggggc tcgggtcgat gccgggggcg ctgctcggcg ggctgctgat cgggctcacc
    16501 gaggcgctgg ggggagtcct gttcacgccg tcggcgaaga gcatgttcgc cttcgggctg
    16561 ctggtgctgg tgctgctgtt ccgcccgcag ggcatcctcg gccgtaaggc cgcggcatga
    16621 ggcacctcct cgacgacgtg ccgcgccgcg gcctgtggtt cctcgcggcg ctgctcgcgc
    16681 tgctgctcgt ctttccgttc ttcgcgagcg ggtacctgct cgccgtcggc ttcctgatcc
    16741 tgtacttcgc ctacgtcggc caggcctgga acgtcatgat gggcttcgcc gggcagctct
    16801 cgctcggcca ctcgctctat gtcggcctgg gcgcctacgc ggccgcggcg ctctacgtgc
    16861 acttcggcgt gagcccctgg gtcgggctcg gcgcctcgat cgcggtgtgc gcgctcgccg
    16921 gcgccgtgat cggctttctc gccttccgct tcggcgtggc gggcgtgtac ttcgccatcc
    16981 tcaccatcgc gttcgcggaa ttcgcgcgca tcggcttcga ccatttcgcc tgggtgcgcg
    17041 gatcctcggg cctctttctg ccggtggcga actacaccga gaacgacctg tggaacctgc
    17101 gcggcagccc gacgatgttc tactacctga tgctcgcgct cacggtcgcg gcgttcgtgc
    17161 tgtgccacgc gctgctccgc agccgcatgg gctactactg gctcgcaatc cgcgagagcc
    17221 ccgaggccgc gcaggcgctc ggcgtgaacc tgttccgcta caagatggcg gcggtggtgc
    17281 tttcggcggc catgacctcg ctcgccggcg tggtgttcgc cttctactac aacaacctct
    17341 ttcccgagca gatcttccac atctcgcgct cgatcgagct gatcctggga ccgatcatcg
    17401 gcggcatcgg cacgctcgcc ggcccgatcg tgggcgcgtt cctgctcacc gggctcgccg
    17461 agggcctgcg cgagctgatg ctgcagctcg gcatcgaggt gcccggcgtg aagcaggtgt
    17521 tctacggact gtgcctgctg ctggtggtga tgttcctgcc ccatggcgtg tggccgccgc
    17581 tcgcgcgccg gctcggcctc gagcgcaagc gctgacatgc tccggatcga gggcctcacc
    17641 aagagcttcc gcgggctgcg cgcggtgcat gacgcgagct tcgcggtgcc cgagggcgcc
    17701 atcgtcgggc tgatcggccc caatggcgcc ggcaagacca cctgcttcaa catgatcgcg
    17761 ggcgtgttcc gccccgacgc cggccaggtg cgcttcgccg gccggcgcat cgacggctgg
    17821 cggcccgacc aggtgtgcgc ggccggcatc gggcgcacgt tccagatcgt gaagccgttt
    17881 gcggggctga gcgtcatcga caatgtggtg gtcggtgcgc tgctaaagtc aaaagatgtc
    17941 tcggcggcga aaagctacgc cgccgagatc ctggaaaggc tggggctcgg agccaagcgc
    18001 gggctccctg cttcatcgct gacgctgccc gaccgcaagc ggctggaagt cgcgcgcgcc
    18061 cttgccacgc gcccgaagct gctgctgctg gacgaggtga tggcgggcct gcggccgacc
    18121 gagtgcgacc agatggtcga agtgttccgc gagctgaacc gcgccgaagg cctcacgatc
    18181 ctcctcatcg agcacgtgat gcgcgcggtg atggcgctgg cgcagacgat cgtggtgctg
    18241 caccacggcg aggtgatcgc gcgcggcgcg ccgcaggacg tggtgcgcga ccccgcggtg
    18301 ctcgagtgct acctgggcga ggaaacgccg gtctgatgct cgccgtcgag aacctcgacc
    18361 tctactacgg cgacgcgcag gcgctcgccg gcgtgtcgct ggaggtgccg cagggcgaga
    18421 tcgtggccat cgtcggcgcg aacggcgcgg gcaagagctc gctcatccgc accatcgccg
    18481 gcatcgagcg cgcgcgcgcc gggcgcatca cgttcaaggg cgccgacctc acgcgcctgg
    18541 agtcgcaccg catctgcaac ctcggcatcg gccaggtggc cgagggccgc caggtgtttc
    18601 cgagcctcac cgtcgaggag aacctgcaga tgggggcact gctgccgcgc gcgcgcgccg
    18661 gcgcggcgcg ggcactcgag gaggcgtacg ccatgttccc gcggctcgcc gagcggcgta
    18721 cgcagcttgc cgggacgctc tcgggcggcg agcagcagat gctcgccatc ggccgctgcc
    18781 tgatgggcca gcccgagctc gtcatgttcg acgaaccctc gctcgggctc gcgccgctcg
    18841 tcgtgcagga ggtgctgcac acgatccgcg cgctgaacgc gcgcggactc acggtgctgc
    18901 tcgtcgagca gaacgtggcc gtgtcgctca agctctcgag ccgcgcctac gtgctcgaga
    18961 acggcagcat cgtgatgcgc ggcaccggcg ccgagctgct gcgcgacgac cgcgtgcggc
    19021 aggcctacct cggtctgtag aatcggagcg tgcagaccga ctacgtgcag cggttcctgt
    19081 tcgagcgcct ggcgatccgc ggacgcctcg tctgcctcac cggcgcctgg acccgaatgc
    19141 tggagggccg cggctatccc gcgcgcattg ccgagacgct cggccacggc acggcgctcg
    19201 cggtgctgct cggtgcgggc cagaagggcg cgggacgggt gacgctgcag gcgcagggct
    19261 cggggccgct caagctgctg gtcgccgatt gcacttcaga gctcaaggtg cgcggcatgg
    19321 cgcattacgc cggcgaggcg cccgagctcg gcgacggccg tctcgccgtc agctacgagg
    19381 acgacgccag cggccggatc taccagagcc tcgtgccgct ggaaggcgcc acgctggccg
    19441 aggtattcga gaattacctg cgccgctcgg agcagcgcga ggcgttcctg cacctgcgcg
    19501 cgagcgatgc ggcgctctgc ggcctgctgc tcgagaagct gccgggcgcc gacgaaaagg
    19561 acgccgacgg ctggaaccgc gtcaccaccc tcgccgcgac gctgacgctg gaggaaacgg
    19621 ttgacgccca gccctacgac ctgctggtga agctgttcca cgaggagctg ctgcgcgtat
    19681 tccggctcta cccggtcgaa taccactgcc cgtggaaccc ggcgagcgtc gagcgcgtgc
    19741 tgagaagcct cggccggccc gaagtggaat cgatcctcgc cgagaaaggc gaggtggtcg
    19801 tgaaaaacga gatgtgcaac cacgaatacc gcttcgaccg cgcggcggtc gaagcggtgt
    19861 tcgcgtagct aggcggcctg cttcggccgc gccagctccg cgcccggcca caaatcaaag
    19921 cgcgcgcgcc ggtactgcgg cgggaactcg accttcacgc cgagccgctc ggccgcgtgc
    19981 cacacccagc gcgggtcgtc gagcaggccg cgcgcgagcg ccaccatgtc ggcgcgcccg
    20041 gcggcgagga tgtcctcggc ctgctgcggg tcggcgatca tgcctaccgc catcacgttc
    20101 atctgcacct tgcgcttcac ctgctcggcg aagccgacct ggtagccggg ctcgaccttg
    20161 atcttctggt gcgccgcctc gccgccgctc gagacgcaga cgtagtccag gccgatcgcc
    20221 ttcagctcgc gcgcgtagac cgccgcatcg tcgacttcga agcccccggg cgcccagtcg
    20281 ctcgccgtga tgcgcgcgcc gagcgccttt tcgcgcggcc aggcctcgcg cagcgcgcgc
    20341 gcgatctcca gcggcgcgcg caggcggttc tccaggctgc cgccatagcg gtcgctgcgc
    20401 ttgttcgaca gcggcgagag gaactggtgc agcaggtagc cgtgcgccga gtgcagctcg
    20461 atgagatcga ggccgatgcg ccgcgcgcgc tgcgccgccg ccgcgaacgc gtcggcgagg
    20521 ccgcgaattt cctccaccgt cagctcgtgc ggcacgtgcc agccttcggc gaacggcacg
    20581 ggcgacggcc ccaccgtctg ccagggcgat tccgccgccg gcagcggctt gccgccgagc
    20641 caggggacct gcgtcgacgc cttgcgcccc gcgtgcgcga gctgcacgcc gatgggcgct
    20701 ttcgagatgc cgcggaatac ctccacgacg cgcttcatcg ccgcctcgtt ctggtcggtg
    20761 tagaggcccg tgcagccgtg cgtgatgcgc gcctcgcgcg tgacgccggt cgcctcgatg
    20821 atcgtgagtc ccgcgcccga gcaggcgagg ctgcccaggt gcacggtgtg ccagtcggtc
    20881 atcgcgccgt cggcggcgct gtactggcac atcggcgaga cgacgatgcg gttgggcagg
    20941 gtgattccgc ccaactggat gggcgtgaag agcttggcgg tcatcaggac ttcctgggct
    21001 tgggtttctt ggggatcttg gggagcgtgg gcacggccac gccgatcagg cagtcgcgcg
    21061 tgacgagtcc ggcgaggcgc gcttcgtccc agcccccggt tgcggcgggg cgcatcggca
    21121 gcaccatgta gcgcatgtcg gcgttcgagt cgtgtacgcg gatctgcacc ttcgcgggca
    21181 ccttcaggcc gaactcggcg agcaccttgc gcggctcgcg caccatgcgg ctgcgatagg
    21241 gcttggactt gtaccactcg ggcggcagcc cgagcaggat ccgcgggtag cacgagcaca
    21301 gcgtgcagac gatcgcgttg tgcgtttcgg gcgtgttctc gaccacgatg agtctcagtc
    21361 ccggcacctc gaagccgagc tcctcggcgg cgcggctgcc gttctcgagc aggcgcgcct
    21421 tgtacgcggg gtcggcccag gcacgcgcca ccatcgccgc gccgcgctcg gggccgcgcg
    21481 cgcgcatcac ggccatcgcg cccgcgatcg catcctcggt gacgatgccc ttcgccacga
    21541 ggagctcgcg caggctcacc tccatcagct ggaagtagtt gtacggctcg tagcgccccg
    21601 tttcctgctt cacggctcga tccagtgctg gaagatctcg agctcgatca cgtcgtcgcg
    21661 gcttccccga tagccgggcc acacgtcgct ctgccggaag cgcacgcggt acagcggctg
    21721 cgccggcagg ccgtcgcgct gctgcgcgag ctcctcgggg ttcggaaagg cgccgcacac
    21781 ccgttccacg acgccggagt gcccgcggat gtagtacggt gttctgacgt ggccgagcgg
    21841 cgcggccttg cgcacgcgca ccttgtcgcc tgcgcggtat ctagcgtcgg gcacgctttt
    21901 cgacctcggc gagcttcctg gcgagctcat ccgtcgtgaa cacgcccctt tgaatcatgg
    21961 tctgcgtgag cgagatcacc cagcggtcgt agtagcccat cttgtcgtag tccgccggcg
    22021 gcagcgactc gatgttcttc cggagctcgt ccaccgtcat gcgcctctcg ccgcccagca
    22081 cgccgacgag cagcatcatc atggcgtcga cgcgcagctc ccagtcgtcg taggcgtgct
    22141 cggtccgccg caccttgccg gccggcagtc cgcccatgtc gtggtggctg cgctcgatca
    22201 cggcccaatg ctactgcagc aggtcgcgcc gcaccatggc gtggacgccc ttgttgccgt
    22261 ccacgagctg cgtgacatgc aggtcggccg cgaggctgca gaagacgtag gcttcggccg
    22321 gtttccagcc cttcatctcc accagccagg cgatcatgcc gcgcagcgcc tgcttcgcgg
    22381 cgtcgtccag gtcttcgttc aggccgatcg tgatgtagtg cgtcggcgtg acggcgcgcg
    22441 gcatggcgag gggcatgtgc ttgtgcagca ccagctcgaa cgtgcccttc aagcaggtct
    22501 cgagcgccgt gaggcagacc tcgccgtcgc cctgcaccgc gtggccgtcg ccggcggaga
    22561 agttcgcgcc cggcacgaac accggcagaa acagcgacgt gcccggcaca tactccttgc
    22621 agtccacgtt gccgccgaac tcgcgcggct cgaccgtgga gatgcgcccg tactcggcgc
    22681 gcggcgcggt ggcgaggatg ccgaagaacg gctgcagcgg aatggtcggg ccccaggcga
    22741 gcttcgccgt catcttgttg cggtcgatcg ggatgacgcg cgcctggaac tcggggaagt
    22801 cctcgggcag cgtgccgcgc aagggccgga tcacgttcca gccccagtcg gcggtgagct
    22861 cgatcgcgcg gatcttcact tccagcgtgt cgcccggcat ggcgcccttc accgcgatcg
    22921 ggccggtgag gatgtgcggc ccgagctggg gcttcaacct ggtgatgatc tcctggtgcg
    22981 cggggctgaa gcgcgtctcg ccgggggcga catcggccca gcccgagacg gtgtcgacca
    23041 ccacggtgtc gccggacgcc acctcgaggc gcggcggcag cttcgcgtcg aagtagcccc
    23101 agtgcacgtt ttccacggaa gggtcgagcc ggtgttgcgc catgacgttc tcctttagtg
    23161 cgcgcgcgcg atgcccagat gctgctcgag gacttcgggc gccttctcca gcgccgccgg
    23221 cgtgccctcg aagacgaagc gacccgccga gaccacgtag atgtagtccg cgaccgacat
    23281 cgcgagcgcg aagttctgct cgacgagcag caccgcgtgg ccgtgcgcgc gcacgtcctg
    23341 catgatgctg cccacctgct gcacgagctt gggcgcgagg ccctcgctcg gctcgtccat
    23401 caggatcagg tcggggttcg ccatcagcgc gcgccccacc gcgagcatct gctgctcgcc
    23461 gccggagagc gcgccgcccg aatgctggat gcgctcggcg agctgcggga actgctcgag
    23521 caccgccggc aggttccagc gcccggggcg cgcccgcgcg aggccgctcg tgggcagcag
    23581 caggttctcg cgcaccgaga gcgagcggaa gatgcgccgg ccctgcggca cgagcgcgat
    23641 gccggcctgc gcgatggcgt acggcggccg gccggcgagc gaggcgccct tgaagcggat
    23701 ctcgccctcg cgcggcggcg tgagcccggc caccgagcgg atgagcgtcg tcttgcccat
    23761 gccgttcctg ccgagaatgg cgacgatggt gccctcgcgc agctcgagcg acagcccctg
    23821 cagcacgtag ctgtcgccgt agtacgtgtg gatgccgcgg accgacagga gagactcagc
    23881 cacgcagcgc ctccccatgg ccgaagtaga cgtccctcac ctgcgcgttg gcgcgcacct
    23941 catcgggcga gccctcgagg atcacgcgtc cctggtgcag cacggtgacg cggtcggcga
    24001 ggccgagcgc cacgtccatg tcgtgctcga tcatgagcag cgtgatgccg cgcggcagcg
    24061 ccgcgatcat ctgcgagatc cgctggcgct cggcgggcga cagtcccgcg cagggctcgt
    24121 cgaggaacaa caccttcgga ttggtgttca gcgcgagcgc gagctcgagc tggcgccgct
    24181 cgccgtagga cacgagcttc gccggcaggt gcgcgcggcc ttcgagcccg acgcgcgcga
    24241 ggccctcgag cgcgcgcgcg cgcacctcgt cgaacgcctc ggccggccgg tgcagcaccc
    24301 acttgcggcg gtcggtgccg acgatggcga gcgcgaggtg ctcgccgagc gtgagatcgg
    24361 ggaacacgtt cgtgatctgg aaggtgcgcc ccatgccgag cgccgtgcgg cggtactccg
    24421 ggtcgtgcgt cacgtcgcgg ccgaacagcg ccacgctgcc gctcgtgggc ttgagcgtgc
    24481 cggtgatgca gtgaaagagc gtggtcttgc cggcgccgtt cgggccgatc agcacgcgcc
    24541 gctcgcccgc ggcgaccgcg agcgacacgc cgtccacggc ggcgagcccg ccgaacacct
    24601 tggcgaggct ttccacgcgc agtgcgggat cagccatggc ggcccttcgc gaggcgcgcg
    24661 cggatgcgct gcgcgatgcc caccagcccc tgcggcgccc agagcaccgt cgcgatgaac
    24721 accgcgccca tgatggcatg gtgcatctcg acgaagctcg agacccagtt gcgcaggccc
    24781 agcaccaccg ccgcgccgag gaacggcccg acgatgctgc cggtgccgcc cacgatcgcc
    24841 atcagcgcgg cttccaccga tacgggaaag gacgacgcta ccgggttcac gaacttgttg
    24901 tagtagacgt agagcacgcc ggcgaagctc gcgagcgccc cggagagcac gaacgccgcg
    24961 tacaggtgca gccccgtgcg gtaacccagg ctctgcatgc gcgtctcgct cgccttgatg
    25021 ccgcgcagcc ccaggccgaa gggcgagcgg atgagcacgc ggtagcccag gccgcacagg
    25081 atcaccgcgg cgagcacgac gtagtagtac tgcgtgtgcg aggtgatggc cgccgggcca
    25141 agcgccggga acggcacgtt ggtcacgccg ttgtcaccgc ccgtgaacga ggaccagcgg
    25201 tgcgccacgc cccacaccac gtagccgagc gcgatggtga tcaggatgaa gtacacgccg
    25261 cgcgtgcgca ccgagatgcc gaacaccgcg gcgagcagcg tgccgagcac gatgcccgcg
    25321 ggcgcggcgg cgatcgtcgg ccagccgtag cgcacctgga ggatcgccac cgtgtaggcc
    25381 gacaccgcga agaaggaggc gtgcgtcagc gagaacagcc ccgcgtggcc gagcagcagg
    25441 tccaccgaaa gcgcgagcag accgaagatc agcatctgcg tggcaagccc cgtccagaac
    25501 ggcccgccga gccacggcag cgccgcggcg aaggcgagcg cgaccacgat cagcgcgagg
    25561 cgggcgcggt ccaccctcat ccctcccgcc cgaacaggcc gcgcggccgg aagatcatga
    25621 gcagcgcaat cggcccgaac agcacgaagt acgaaaactc cggcagcagc cagcgcccgt
    25681 aggcatccag cagccccacg atcagcgcgc cgatcatcgc cccttcgagg ctgccgaggc
    25741 cgccgacgat cacgacgacg agcgcgtaca ccaggatctc ccattccgcg cccgggtaca
    25801 gggtcagaaa cgcgccgccc gcgacgcccg ccatgccggc gaggaacgac gccagcgccg
    25861 agaccatcac gaacaggcga tccacgttga tgccggtggc gttcacctgc tcgcggtcgt
    25921 ccacgcccgc gcgcaccacg gcgccgagct gtgtcttctt gtagagcagc cacagcgcgg
    25981 cgcccacggc gagaccgagg aagatgagca cgaagcggta cttcgggtag tagatgtcgc
    26041 cgggcagcac caccgccccc ttgaggaact gcggcgcggg caccatcagg ttgtcgccgc
    26101 cccagatctg cagcgcgaca tcgccgatca cgaaagcgat gcccaccgtg agcagcacct
    26161 gcgcgaggtg gttctcgccc gtgcgccgga tgaggaagcg gtcgatgaag tagccgacga
    26221 cgatgatggc ggccccgccg ccggcaagcg cgagcgcgaa gctgccggtc ttctcggcga
    26281 tcgtgaggcc gatgtagccg ccgaggaggt aatacgcgcc gtgcgccatg ttgacgacgc
    26341 gcatcaggcc gaacgacagc gtgaacccgc tcgccagcag gaacagcagc gccgcgagcg
    26401 agacgccgtt gatcgcctgg agaatggcgc tcgtcatcgc ggcgcgggca agaaaaaggg
    26461 cgggcgtcgc cgcccgccct cgtcgcggct catgcgccta gcgcttgtac ggcccgcgcg
    26521 cgaggaactc cttcggatcg aaggtccaga actgcgacac gccctcgtag gtcttgaagg
    26581 gcacgttggt cagcacctcg cccagcaccg ggtgcttgat cttctggatc ttggagatgt
    26641 agacgttctg gatcgggttg ccgtagtcat ccatcctgag cggcccgcgc ggtcccttga
    26701 tggtcacggt gcgcatcgcg ttcaggaacg cagcgcggtc ctcgaccttg ccgccgatct
    26761 tgtcgatcgc ggccttggtc cacacgcccg cggagtaggc ggactcgccg aaccaggacg
    26821 gcagccggtt ggtgcgcttg gcgaactccg tggtgaaggc gatgttctcg ggcgtctgca
    26881 ggcccgcggc gtagttgagc gagttgccga tcatgccgat ggcgcggtcg tccatctgcg
    26941 gcagcacgtc ctggtgcatc cagtagccgc cgtagatctt cttcttcttg tgcatgccga
    27001 agttgaacca ggcctcgaac aggcgcacgc ggtccgggcc gaccacgacc gcggaaacgc
    27061 cgtcggtgtc ctcggggatc gcggcgatcg tcgggccgta gtccttggtg ccgatcgggt
    27121 tccagatcat cttggcgacc ttgccgccga gcgcggtgaa cgtgcgcacc gcgccgagcg
    27181 tcacttcatg cccccaggtg tagtcctggc cgatgaaggt gacgttcctc aggccgagct
    27241 ccttgtacat gtactcgccc caggggtggc cgatctgcga cgccgagacc gcggtgcgca
    27301 ccacggtcgg gctgcgatcc cacttggtga tcacgtcggc gccggccgcg tccatgacga
    27361 gcggcacgcc ggtctccttg ctcacctgcg ccacagccgg gccctcgtgg ccgcagagcg
    27421 gcccgaccat gaagtgcacc ttttcctggt gcacgaggcg gcgcgcctgg gtaagcgcct
    27481 ggtcgggatt gcaggtggtg tcggcgatca cgtattcgac cttgcggccg cccgcgctgt
    27541 ggccggcctg gtcccagaac atcttccagc catcgaccat gtccttgccc ggcgtggcga
    27601 gcggaccggt gaccggggcg agcacgccga tcttgatcgg cccgctctgc gcgtgcgcgc
    27661 cggtgcccat gccgatggcg gcgagcagcg cgacgatgct gcccagcgaa acattcattt
    27721 tgagcgattt cattgcgtct cctcctgaag gtcgggcaat tatactgggc gcatgaccga
    27781 cgatcaccag cgtttcggca gcagccgcgg cgcgccgcgc agcgaggacg atcccctgct
    27841 cgcgggccgc gggcgcttca ccgacgacct gaagacggcg cagcacgcgc gcgcggcgtt
    27901 cgtgcgctcg ccgatgggcc acgcgacgat ccgtgccgtc gatgcgaaag cggcggcgcg
    27961 gatgccgggc gtgctcgcga tcctggccgg cgccgacctg gcgctcggcg cgatcccgcc
    28021 gggggtcctg ctcccgggcc gggacggcaa gccgatgttc gccaccgcga tgccggtgct
    28081 ggcgacggac cgcgtgcgct acgtcggcga gccggtggcg ctggtcgtcg ccgaaaccct
    28141 gtcccaggcg ctcgatgcgg cggacgcggt gcaggtcgag tacggggagc tgcccgccgt
    28201 gtccacggtg gcgcgcgcgc tcgccaaagg cgc
//
LOCUS       DBNHKE010000142         5254 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_130295, whole genome shotgun sequence.
ACCESSION   DBNHKE010000142 DBNHKE010000000
VERSION     DBNHKE010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_130295"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="AC16O9_09665"
     CDS             <1..601
                     /locus_tag="AC16O9_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6516923.1"
                     /translation="AALAASRPAPRVHPAVASGYAAYQAGDMERARADYETALRDEPG
                     NRDALLGLAAVETRAQRFAAAEALYRRLLLADPRDPHAHAGLLALRSQQLDPVTAESR
                     VKGLLAVDPDASVLHFTLGNQFAQQRRWAEAQQAYAKAVAMDPDNPDYAYNHAVSLEH
                     TRQPQPALQQYRRALALALQRTASFDAAAAQARVQQLSN"
     gene            651..2360
                     /locus_tag="AC16O9_09670"
     CDS             651..2360
                     /locus_tag="AC16O9_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6516924.1"
                     /translation="MNSPTNPQAKRHIGQILIDQGILTEDQLRIALLEQTKSKAPVGR
                     LLVQLGFVSEATLRDALSEKLGLQSVDLAHIIVDPAALKMLPRDMARRYRMFPVALDR
                     QMKKFIVALADTNNIVAVDQLRAHLKGDLEIELRLAGDSEIERAVEHYYGHEFSIDGI
                     LREIETGEVDYATIAEGDEYSQPVVRLISALLADAVERGASDIHFEPEQNFLRIRYRI
                     DGVLRQIRSLHKTYWPAMAVRLKVMAKMNIAETRAPQDGRISATMSGRVVDFRVASLP
                     TTHGENIVLRILDRQKGIVPLDKLGLEDGALELLKRMIARPEGIIMVTGPTGSGKTTT
                     LYSILNHINSDGINIMTLEDPVEYPMTLIRQTSVSEAAKLDFANGVRALMRQDPDAIL
                     VGEIRDKDTAEMAFRAAMTGHQVYSTLHTNSAVGAIPRLLDIGIVPDVMSGNIIGVVA
                     QRLVRKLCRTCRQSYDPEVRERRTLGMTPAEPAVLYRAVGCDQCDYQGYRGRQSILEI
                     LKIDGGLDELIARRATAREILTAARAKGFRTLADDGVRMVRAGLTSLDELMRVVDLTD
                     RMA"
     gene            2373..3578
                     /locus_tag="AC16O9_09675"
     CDS             2373..3578
                     /locus_tag="AC16O9_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6516925.1"
                     /translation="MPLYTYKAIDAQGKSVIGRTEAVNPFDLEQRLLRMGLDLVYGAP
                     TSQTARLIGGARISRQDLINFCFHLEQLANAGVPVVEGLVDLRESVENRRFREVISGL
                     LESIEGGKNLSQSLAEFPEVFGKVFVSLVRSGEQTGRLAEVLKSLNESLKWEDELAAQ
                     TKKIIMYPAFVGSIVLLVTFFLMIYLVPQMTGFIRNMGQNIPLQTQILIAVSNFCINY
                     WWVVIAAPFVLWFGVKFAARANPAVAYTLDRYKISLPLVGPILKKIILSRFASSFAMM
                     YSSGITVLDAIRSCEEVVGNRPVEYALRGAGQQIAEGKNLTVAFQDAGLFPPLVIRML
                     RIGENTGALDTALLNVSYFYNREVRESIGKVQAMIEPALTVVLGLILGWVMLSVLGPV
                     YDTISKMKF"
     gene            3580..5136
                     /locus_tag="AC16O9_09680"
     CDS             3580..5136
                     /locus_tag="AC16O9_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516926.1"
                     /translation="MKRLIYFTADDHTVHTWSRGRLAVEAKFPGDDLGVTAFREYLAQ
                     HRGALFYAVADLAGEDFHEEMIPYLRGADRELVVQRRLAQRYRDTRLAAALSLGQVRG
                     ERRNERLLLTSFTNTQRLTPWLDALAELEAKLAGVFSVPLLAPALAGRLGAGRGRSFV
                     VTANSAGLRQCYVDEGRLRFARLERTVDMAPEAFAAFVRSETGRLAQYLSTLRVLPRE
                     GPPIQVIVVAPPGQRGAFERTLVSDARLSFLTIDQDEAVRRVGVKSTVPGQGAEQLYL
                     HLAVRRPPRDQFARREDRRSYVLWQLRRAVLAAGALGLAGCALYAGASWLDIAGTRAE
                     ADRVQLETQRAREEYQRITSAFPVTQTTTDNLRATVVEFTKIAQQTAPPERALVYLSR
                     VLEDFPLIELDALQWRVEGKGTRGARARTSAPPAGPAAAAEAAPTTSEIFEISGRVNT
                     ARRSDYRAITAEVQRFADALRADPAYQIERTQLPFDVTSEGTLSGDISTTTETGEAPR
                     FTIVLTRSIR"
BASE COUNT          837 a         1862 c         1745 g          810 t
ORIGIN      
        1 tgccgccctg gccgccagcc gcccggcgcc gcgcgtgcat ccggccgtgg cctccggcta
       61 cgccgcctac caggccggcg acatggagcg tgcgcgcgcc gactacgaaa ccgccctgcg
      121 cgacgagccc ggcaatcgcg atgccttgct cgggctcgca gcggtggaaa cgcgggccca
      181 gcgcttcgcg gcggccgagg ccctgtaccg tcgtctgctg ctcgccgacc cccgcgaccc
      241 gcacgcgcac gcgggcctgc ttgcgctgcg ctcgcagcaa ctcgatccgg tgaccgcgga
      301 gtcgcgcgtg aagggactgc tcgccgtcga tcccgacgca agcgtgctgc atttcacgct
      361 cggcaaccag tttgcgcagc agcgccgctg ggccgaggcg caacaggcct acgccaaggc
      421 cgttgccatg gatccggaca accccgatta cgcctacaac cacgctgtca gcctggagca
      481 cacgcgccag ccgcaaccgg cgctgcagca gtaccgtcgt gcgctggcac tcgccctgca
      541 gcgtaccgcc agcttcgatg ccgccgccgc gcaggcgcgt gtgcagcagt tgtcgaactg
      601 acacatccta aagcccggtt ttttcgggct ttctgtatca tgcagattgg atgaattcgc
      661 ccaccaaccc gcaggccaag cgccatatcg ggcagatcct gatcgaccag ggcatcctga
      721 ccgaggacca gctgcgcatc gcgctgctgg agcagaccaa gtcgaaggcg cccgtgggtc
      781 gcctcctggt gcagctgggc ttcgtgtccg aagccacgct acgcgacgcc ctgtcggaaa
      841 agctcggcct gcagtcggtc gacctcgccc acatcatcgt cgacccggcg gccctcaaga
      901 tgctgccgcg cgacatggcg cggcgctacc gcatgttccc ggtcgcgctc gaccgccaga
      961 tgaagaagtt catcgtcgcg ctggccgaca ccaacaacat cgtcgccgtc gaccagctgc
     1021 gcgcgcacct gaagggcgac ctcgagatcg agctgcgcct cgccggcgac tccgagatcg
     1081 agcgcgccgt cgagcactac tacggccacg agttctcgat cgacggcatc ctgcgcgaga
     1141 tcgagaccgg cgaggtcgac tacgccacca tcgccgaggg cgacgaatac tcgcagccgg
     1201 tggtgcgcct catctcggcg ctgctcgccg atgccgtgga gcgcggcgcc tccgacatcc
     1261 acttcgagcc cgagcagaac ttcctgcgca tccgctaccg catcgacggc gtgctgcgcc
     1321 agatccggtc cctccacaag acctactggc cggcgatggc cgtgcggctc aaggtgatgg
     1381 cgaagatgaa catcgcggag acgcgcgcgc cgcaggacgg gcgcatctcg gcgaccatgt
     1441 cggggcgcgt ggtggacttc cgcgtggcga gcctgcccac cacccacggc gagaacatcg
     1501 tgctgcggat cctcgaccgg caaaagggca tcgtgccgct cgacaagctg gggctcgagg
     1561 acggcgccct ggagctcctg aagcgcatga tcgcgcggcc cgaggggatc atcatggtga
     1621 cgggccccac cggcagcggc aagacgacga cgctctactc gatcctcaac cacatcaact
     1681 ccgacggcat caacatcatg acgctggagg atccggtcga gtacccgatg acgctcatcc
     1741 ggcagacttc ggtgtcggaa gccgccaagc tcgatttcgc caacggcgtg cgcgccctga
     1801 tgcgccagga ccccgatgcg attctggtgg gcgagatccg cgacaaggac accgcggaga
     1861 tggcgttccg cgcggcgatg accggtcacc aggtgtattc cacgctgcac accaactcgg
     1921 cggtgggcgc gatcccgcgg cttctcgaca tcggcatcgt gcccgatgtg atgtcgggca
     1981 acatcatcgg cgtcgtggcg cagcgcctgg tgagaaagct gtgccgcacc tgccgccagt
     2041 cctacgaccc ggaagtgcgg gagcggcgca cgctcggcat gacgccggcg gagccggcgg
     2101 tgctgtaccg cgccgtgggc tgcgaccagt gcgactacca gggctaccgc gggcgccagt
     2161 cgatcctcga gatcctgaag atcgacggcg gcctggacga gctgatcgcg cgccgcgcca
     2221 ccgcgcgcga gatcctcacg gcggcgcgcg ccaagggctt ccgcacgctc gccgacgacg
     2281 gcgtgcgcat ggtgcgcgcc ggcctcacca gcctggacga gctgatgcgc gtcgtcgatc
     2341 tcacggaccg catggcgtaa aggggctgcg acatgcccct ctacacgtac aaggccatcg
     2401 acgcccaggg caagagcgtg atcggccgca ccgaggcggt caatccgttc gacctcgagc
     2461 agcggctgct gcgcatgggc ctcgacctgg tctacggcgc gcccaccagc cagaccgcgc
     2521 ggctgatcgg cggcgcgcgc atctcgcgcc aggacctgat caatttctgc ttccacctgg
     2581 agcagctcgc caacgccggc gtgccggtgg tcgaaggcct cgtcgacctg cgcgagagcg
     2641 tcgagaaccg gcgcttccgc gaggtgatct cgggactgct ggagtcgatc gaaggcggca
     2701 agaacctgtc gcagtcgctc gccgaatttc ccgaggtgtt cggcaaggtg ttcgtcagcc
     2761 tggtgcgctc gggcgagcag accgggcggc ttgccgaggt gctgaagagc ctcaacgagt
     2821 cgctgaaatg ggaggacgag ctcgccgcgc agaccaagaa gatcatcatg tacccggcgt
     2881 tcgtcggctc gatcgtgctg ctggtgacct tcttcctgat gatctacctg gtgccgcaga
     2941 tgaccggctt catccgcaac atgggccaga acatcccgct gcagacgcag atcctgatcg
     3001 cggtctccaa tttctgcatc aactactggt gggtggtgat tgcggcgccg ttcgtcctct
     3061 ggttcggcgt caagttcgcc gcgcgcgcca acccggccgt ggcctacacg ctcgaccgtt
     3121 acaagatctc gctgccgctg gtcgggccga tcctgaagaa gatcatcctg tcgcgcttcg
     3181 cctcgagctt cgccatgatg tactcctcgg gcatcacggt gctcgacgcc atccgcagct
     3241 gcgaggaggt ggtcggcaac cggcccgtcg agtacgcgct gcgcggggcc gggcagcaga
     3301 tcgccgaggg caagaacctc accgtcgcgt tccaggacgc ggggctgttt ccgccgctcg
     3361 tcatccgcat gctcaggatc ggcgagaaca ccggcgccct cgataccgcg ctgctcaacg
     3421 tgagctactt ctacaaccgc gaggtgcgcg aatcgatcgg caaggtgcag gcgatgatcg
     3481 agccggcgct gacggtggtg ctgggcctga tcctcggctg ggtcatgctg tcggtgctgg
     3541 ggccggttta cgacacgatc agcaagatga agttctgaca tgaagcgcct gatctacttc
     3601 accgccgatg accataccgt gcacacgtgg tcgcgcggca ggctcgccgt ggaggcgaag
     3661 ttcccgggcg acgatctcgg ggtcacggcg tttcgcgagt accttgcgca gcaccgcggc
     3721 gcgctgttct acgcggtggc cgacctggcc ggcgaggact tccacgaaga gatgatcccg
     3781 tacctgcgcg gcgccgaccg cgagctggtg gtgcagcgac gcctggcgca gcgctaccgt
     3841 gatacgcggc tcgccgcggc gctgtcgctc gggcaggtgc gcggcgagcg ccgcaacgag
     3901 cggctgctgc tgacctcgtt taccaacacg cagcgcctga cgccctggct cgacgcactg
     3961 gcggaattgg aggccaagct cgccggcgtg ttttcggtgc cgttgctcgc gccggcgctc
     4021 gccgggcgcc tgggcgcagg ccgcgggcgc tccttcgtgg tcaccgccaa cagcgccggc
     4081 ctgcgccagt gctatgtcga tgaaggccgg ctgcgcttcg cgcgcctgga gcgcaccgtg
     4141 gacatggcgc cggaggcttt cgccgccttc gtgcgctcgg aaaccgggcg cctggcccag
     4201 tacctgtcca cgctgcgcgt gctgccgcgc gaaggcccgc cgatccaggt gatcgtggtc
     4261 gccccgccgg gccagcgcgg cgccttcgag cgcacgctgg tctccgacgc gcgcctttcg
     4321 ttcttgacca tcgaccagga cgaggcggtg cggcgcgtgg gcgtcaaatc cacggtgccc
     4381 ggacagggtg ccgagcagct ctacctgcac ctggcggtgc gccggccgcc gcgcgaccag
     4441 ttcgcgcggc gcgaggaccg gcgcagctac gtgctgtggc agctgcggcg cgcggtgctg
     4501 gccgcgggcg cactgggtct cgctggctgc gccctttatg ccggcgcgag ctggctcgat
     4561 atcgccggca cgcgcgcgga agccgatcgc gtgcaactcg agacgcagag ggcgcgggag
     4621 gaataccagc gcatcacctc cgctttccct gtcacgcaga ccaccaccga caacctgcgc
     4681 gcgaccgtcg tcgaattcac caagatcgcg cagcaaaccg cgccgcccga gcgtgccctg
     4741 gtctacctgt cccgcgtact cgaggatttc ccactcatcg agctcgacgc cctgcagtgg
     4801 cgcgtcgagg gcaagggtac ccgcggcgcg cgcgcacgga catcggcgcc gcccgccggg
     4861 cccgccgcgg cggcggaagc cgcccccaca acgagcgaga tcttcgagat ttctggccgc
     4921 gtcaacaccg cacggcgctc ggactaccgc gccatcacgg ccgaggtgca gcgctttgcc
     4981 gacgcgctgc gcgccgatcc cgcctaccag atcgagcgca cgcaactgcc gtttgacgtc
     5041 acgtcggagg gcacgctgtc cggcgatatc agcaccacca cggaaaccgg cgaggcgccg
     5101 cgtttcacga tcgtgctgac gcggagcatc cggtgagctt ctcggcgcac gagctgcgac
     5161 agctcgccct gcccctgctg gcgtgcctcg cgctgctcgg cgcgggctcg gcgctcatca
     5221 cttggaccga ggccaggaac gccgctgcgg accg
//
LOCUS       DBNHKE010000143         6404 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1241474, whole genome shotgun sequence.
ACCESSION   DBNHKE010000143 DBNHKE010000000
VERSION     DBNHKE010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1241474"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..708)
                     /locus_tag="AC16O9_09685"
     CDS             complement(217..708)
                     /locus_tag="AC16O9_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6516927.1"
                     /translation="MAASDAEGRRSLAAYMLRAPFSLEKTSYDAASGTVIYRSRLHAT
                     LKRNFQLMPGVQWLELLCKHIPDRHEHLVRYYGRYSSRTRGAERERPEIDEPEADSAG
                     PARQAAKAAWAKLIRKVYEVDPLLCPECGTQMRVIALIEDPAVIERILCWLGLWEPLP
                     EIA"
     gene            complement(739..3435)
                     /gene="ppc"
                     /locus_tag="AC16O9_09690"
     CDS             complement(739..3435)
                     /gene="ppc"
                     /locus_tag="AC16O9_09690"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR6516928.1"
                     /translation="MNLDATEQALREDRRLLGRLLGEVIREQAGAATFDTVEQVRQSA
                     VGFRRAEGEDGAAAATAKAALEAQLDALDIEQTLHVVRAFSYFSLLLNIAEDAHRDRQ
                     APGGPGSFAHALARARQAGATDEALAAWCARARVSPVLTAHPTEVQRQSVLDCEREIA
                     RLIAQPAGEARDAALGREVLRLWLTSMLRLTKLDVADEIRNGLAYFQLSLFEELPRLY
                     AQIEAALGAACLPSFLWIGTWIGGDRDGNPNVGAATLGLALAQQARLALGHYLEELHE
                     LGRELALSTRIRAAPPEVAAAAAASGDDSPYRSDEPYRRIVSGIYARLAATARALCAL
                     EAVPAPSVARPAYAGPAELAADLALIERGLEAQGAGRLASGRLRALQRKVEVFGFHLA
                     RIDLRQSSGEHEAVVAELLARAGVEAGYGGLDEAARVALLSRELAGPRPLRSPHLTYS
                     ELAESELAVVAAAAEGRRRLGPHAVPHYVISHCQSVSDLLEVGVLLREAGLLRPGDPP
                     ALDMDIVPLFESVADLERCGEIMAAALEQPLYRSWVRARGDEQEVMLGYSDSNKDGGY
                     LASNWALYKAAAALRAACASRGVRLRLFHGRGGTVGRGGGPSYDAILAQPAGSVDGAL
                     RLTEQGEVIASKYADPGSGRRNLEILAAATLEASLGRRAPHPNRERHMEIMQALAGHA
                     LRAYRALVRQPGFLDYFRASTPLEEIAALNIGSRPASRRGSARLEDLRAIPWVFSWSQ
                     CRLMLPGWYGFGSAVEAWLGEKGGTLDELREMQRGWPFFRSVLSNMDMVLAKSDLAIA
                     SRYAELVPDAKLRGEVFGRISGEWQRTWRFLLEITRQTELLADNPALARSIRNRFPYL
                     DPLNHLQVELLRRYRAGETDERTRRAIHLTINGVAAGLRNSG"
     gene            complement(3446..3808)
                     /locus_tag="AC16O9_09695"
     CDS             complement(3446..3808)
                     /locus_tag="AC16O9_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516929.1"
                     /translation="MARALACLLVALAAALPARADYEAGLAAARAKDYVTALREWRPL
                     AEQGHRAAQFNVGLFYAVGRGVAPDPVPAVAWITVVQENGHQRTALFETLKQHMSAAQ
                     LREAENLAEMVRRQCRVD"
     gene            complement(3810..4655)
                     /locus_tag="AC16O9_09700"
     CDS             complement(3810..4655)
                     /locus_tag="AC16O9_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6516930.1"
                     /translation="MRFTGSADYVSTEDLTLAVNAAITLQRPLLVKGEPGTGKTMLAV
                     QVARALGVPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDARVHDIHNYIVKGMLWQ
                     AFSSDKPVVLLIDEIDKADIEFPNDLLRELDRMEFYVYETKELVKAKQRPIVFITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMERIVGVHFPSLKKSLLREAMEVFFEVREVPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSNDRKTIIPPLHGALLKNEQDVHLFERLVFMT
                     RAKGG"
     gene            4720..5037
                     /locus_tag="AC16O9_09705"
     CDS             4720..5037
                     /locus_tag="AC16O9_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6516931.1"
                     /translation="MLARTVLAVTLAALCGAAAAQKPAADPAHDKVSMCVGCHGIPGY
                     KTAYPVVYHVPKIGGQNPAYIANALKAYRAGERWHPSMRGIAASLSDKDIADIAAYYG
                     GSK"
     gene            5034..5360
                     /locus_tag="AC16O9_09710"
     CDS             5034..5360
                     /locus_tag="AC16O9_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6516932.1"
                     /translation="MTRLLFAMLALALAGAAQAGGNAAAGKDKAAQICAACHGPSGAK
                     PTAPENPILAGQHYDYLVKALQDYKSGKRNNAIMKGFAASLSKQDIEDLSAWFASQAS
                     GLHYQR"
     gene            complement(5357..5992)
                     /gene="nth"
                     /locus_tag="AC16O9_09715"
     CDS             complement(5357..5992)
                     /gene="nth"
                     /locus_tag="AC16O9_09715"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR6516933.1"
                     /translation="MTPAKRHELFRRLRAADPEPKSELQYRTPFELLVAVVLSAQATD
                     KSVNLATPKLFEAAPTPQAMVALGEARVTELIRTIGLFRTKAKNVVALSRLLVERHGG
                     EVPASREALQALPGVGRKTANVVLNVAFGEPTIAVDTHIFRVANRTGLAPGKTPEAVE
                     LKLLQAVPAQFRRHAHHWLILHGRYVCLARKPGCPQCAIRDLCGFREKTRA"
     gene            complement(5989..>6404)
                     /locus_tag="AC16O9_09720"
     CDS             complement(5989..>6404)
                     /locus_tag="AC16O9_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR6516934.1"
                     /translation="RSGHRPAGARWQGRARAARARHRPMKRATVARIDEARCIGCARC
                     IDACPVDAIVGARGFMHTVVAELCIGCDLCLPPCPVDCIDVVPANAPWTAADARAAKQ
                     RAAARKRRLARQAEVPASDLQRRRAILAAALAKRR"
BASE COUNT          920 a         2290 c         2248 g          946 t
ORIGIN      
        1 ataggaactt ccttcctcgt tgggctgcgc aactggcgtg gctcgcggcg gcgaggccgc
       61 gattcggggc tcgggggctg aaggttcgag cggcgcgcag gcaaggcgcc gcggtagcgg
      121 cggggcaacc ggcgttatgg caaataaccg cgcccctttt gcgcgtttcg gcagcttcgg
      181 caagagcgca gcccgcgagc gccgcttggg gcgggctcag gcgatctcgg gcagcggctc
      241 ccaaaggccc agccagcaga ggatgcgctc gatcacggcc gggtcttcga tgagcgcgat
      301 cacgcgcatc tgtgtcccgc attccgggca aaggagcgga tcgacctcgt agaccttgcg
      361 gatcagcttc gcccaggcgg ccttcgcggc ttggcgtgcc gggcccgcag agtcagcctc
      421 gggctcatcg atctccgggc gctcgcgctc ggcgccgcgc gtgcggctgg agtagcgccc
      481 gtagtagcgc accaggtgct cgtggcggtc ggggatgtgc ttgcagagga gctccagcca
      541 ctgcacaccc ggcatgagct ggaagttgcg tttgagcgtc gcgtgcagcc tgctgcggta
      601 gatcaccgtg ccgctggctg cgtcgtagga ggtcttctcc agcgagaacg gcgcgcgcag
      661 catgtaggcg gcgaggctcc tgcggccctc ggcatcgctt gcagccacgc gcactgcgtt
      721 gtgcacgtaa acccaccgct acccgctatt cctcaacccc gctgccaccc cgttgatggt
      781 caagtgaatc gcccgccgcg tgcgctcatc ggtctcgccc gcgcgatagc gccgcaatag
      841 ctccacctgc aaatggttta gcggatccag gtacggaaac cgattccgta tcgagcgcgc
      901 cagcgcgggg ttgtcggcca gcagctccgt ctgcctggtg atctctaaca agaatcgcca
      961 cgtgcgctgc cactcgcccg aaattctccc gaacacctcg ccacgcaact tcgcatccgg
     1021 cacgagctcc gcataccgcg aggcgatcgc caagtccgac ttcgcgagca ccatgtccat
     1081 gttggaaagc accgaccgga agaagggcca cccccgctgc atctcgcgca gctcgtccag
     1141 cgttccgcct ttctccccga gccaggcctc gacggcggag ccgaatccgt accacccggg
     1201 gagcatcagc cggcactggc tccagctgaa cacccagggg atcgcgcgca ggtcctcgag
     1261 ccgcgcggag ccgcgccgcg aggccggccg gctgccgatg ttgagcgcgg cgatctcctc
     1321 gagcggggtg gaggcgcgga agtagtccag gaagcccggc tgccgcacca gcgcccggta
     1381 ggcgcgcagc gcatgacccg ccagagcctg catgatctcc atgtgccgct cgcggttcgg
     1441 atgcggcgct cgccgcccga ggctcgcctc caaagtggcg gcagccagga tctccaggtt
     1501 ccgccgcccg ctcccgggat cggcgtactt gctcgcgatg acctcgccct gctcggtgag
     1561 ccgcagcgcg ccgtccaccg accccgcggg ctgcgcgagg atcgcgtcgt agctcggccc
     1621 gccgccacgc cccacggtgc cgccgcgccc gtgaaagagc cgcagccgca cgccgcggct
     1681 cgcgcaggcc gcgcgcagcg ccgcggccgc cttgtacagc gcccagttgg aggcgaggta
     1741 gccgccgtcc ttgttgctgt cggagtagcc gagcatgact tcctgctcgt cgccgcgtgc
     1801 gcgcacccag gagcggtaga gcggctgctc gagcgccgcg gccatgatct cgccgcagcg
     1861 ctcgaggtcg gcgaccgact cgaagagcgg cacgatgtcc atgtcgagcg ccggcgggtc
     1921 gcccgggcgc agcagtccgg cctcgcgcag cagcacgccg acttcgagca ggtccgacac
     1981 cgactggcag tgcgagatga cgtagtgcgg cacagcgtgc ggcccgagcc ggcgccggcc
     2041 ctcggcggcc gcggccacca cggcgagctc ggattcggcg agctcggaat aggtgaggtg
     2101 cggcgagcgc aaggggcgtg ggccggcgag ctcgcgcgag agcagcgcga cgcgcgcggc
     2161 ttcgtccagc ccgccatagc cggcttcgac tcccgcgcga gcgaggagct ccgccaccac
     2221 cgcctcgtgc tcgcccgagc tctggcgcag gtcgatgcgc gcgaggtgaa agccgaacac
     2281 ctccaccttg cgctgcagcg cgcgcaggcg cccggacgcg aggcgcccgg cgccttgcgc
     2341 ctccagcccg cgctcgatga gcgcgaggtc cgccgcgagc tccgcgggcc cggcgtaggc
     2401 ggggcgcgcg acgctcggcg cgggcacggc ctcgagtgcg cagagcgcgc gcgcggtcgc
     2461 ggcgagccgc gcgtagatgc ccgagacgat gcgccggtag ggctcgtcgc tgcggtacgg
     2521 ggaatcgtcg cccgaggcgg ccgcggccgc cgcgacctcc ggcggcgccg cgcggatgcg
     2581 cgtggagagc gcgagctcgc ggccgagctc gtgcagttcc tccaggtagt gcccgagcgc
     2641 gaggcgcgcc tgctgcgcga gcgcgagccc gagcgtcgcg gcgccgacgt tggggttgcc
     2701 gtcgcggtca cccccgatcc aggtgccgat ccagaggaag gagggcaggc acgccgcgcc
     2761 gagcgccgcc tcgatctggg cgtagagccg cggcagttcc tcgaacaagc tgagctggaa
     2821 gtaggcgagg ccgttcctga tctcgtcggc gacgtcgagt ttcgtgagtc gcagcatcga
     2881 ggtgagccac aggcgcaaca cctcgcgccc gagcgccgcg tcgcgcgcct cgcccgccgg
     2941 ctgcgcgatg agccgcgcga tttcccgctc gcagtcgagg accgactggc gctgcacctc
     3001 ggtggggtgg gcggtgagca cggggctcac gcgcgcacgg gcgcaccagg cggcgagcgc
     3061 ctcgtcggtg gcccctgcct gccttgcgcg cgccaacgcg tgcgcgaagc tccccgggcc
     3121 gccgggcgcc tgccggtccc ggtgcgcgtc ctcggcgatg ttgagcagca gcgagaagta
     3181 gctgaacgcg cgcaccacgt gcagcgtctg ctcgatgtcg agcgcgtcga gctgcgcctc
     3241 gagcgccgcc ttcgcggtgg ccgctgcggc gccgtcctcg ccctcggcgc ggcgaaagcc
     3301 gaccgcgctc tggcggactt gctccacggt gtcgaaggtg gccgcgcccg cctgctcgcg
     3361 gatgacctcg ccgagcagac gcccgagcag gcgccggtcc tcgcgcagtg cctgctctgt
     3421 ggcgtctagg ttcaacgtcg cggcgtcaat cgacccggca ctgccggcgc accatttccg
     3481 ccaggttctc ggcctcgcgc aattgcgctg cgctcatgtg ctgcttcagg gtttcgaaca
     3541 gtgccgtgcg ttggtgtccg ttttcctgca ccaccgtgat ccaggccacc gccggcacgg
     3601 ggtcgggcgc aacgccccgc cccacggcgt agaacagtcc cacgttgaat tgcgcggcgc
     3661 ggtgtccctg ctcggcgagc ggccgccact cgcgcagcgc agtcacgtag tccttcgcgc
     3721 gcgccgcggc gagccccgcc tcgtaatccg cccgcgccgg cagcgccgcg gcgagcgcga
     3781 cgagcaggca ggcgagggcg cgcgccacgc tagccgccct tggccctcgt catgaacacg
     3841 agccgctcga agaggtgcac gtcctgctcg ttcttgagca gcgcgccgtg cagcggcggg
     3901 atgatggtct tgcggtcgtt cgagcggagc gcctccggcg ggatgtcctc ggcgagcagg
     3961 agcttcagcc agtccagcag ctccgaggtc gagggcttct tcttcagtcc cggcacctcg
     4021 cggacctcga agaaaacctc catcgcctcg cgcagcagcg acttcttcag gctcgggaag
     4081 tgcacgccga cgattcgctc catcgtttcc ttgtcgggga agcggatgta gtggaagaag
     4141 cagcgccgca ggaacgcatc gggcagctcc ttctcgttgt tcgaggtgat gaagacgatc
     4201 gggcgctgct tcgccttgac cagctccttg gtctcgtaga cgtagaactc catgcggtcg
     4261 agctcgcgca gcaggtcgtt cgggaactcg atgtcggcct tgtcgatctc gtcgatcagc
     4321 agcaccacgg gcttgtcgga cgaaaacgcc tgccacagca tgcccttgac gatgtagttg
     4381 tggatgtcgt gcacgcgcgc gtcgccgagc tgcgagtcgc gcaggcgcga cacggcgtcg
     4441 tactcgtaca ggccctgctg cgccttggtg gtggacttga tgtgccactc gagcaggggc
     4501 acgccgagcg ccctggccac ctgcacggcg agcatcgtct tgccggtgcc gggctcgccc
     4561 ttcaccagca gcggccgctg cagggtgatc gcggcgttca cggcgagcgt gaggtcctcg
     4621 gtggaaacgt agtcggccga tccggtgaag cgcattgccg tccctcggga aaatcgcatt
     4681 ataatcgcgc ggtcctttcc tctgtccgat caccacaaaa tgctcgcacg caccgttctt
     4741 gccgttacgc tcgccgcgct ctgcggcgcc gcggcggcgc agaagccggc ggcggacccg
     4801 gcgcacgaca aggtgtcgat gtgcgtcggc tgccacggca tccccggcta taagaccgcg
     4861 taccccgtcg tctatcacgt gccgaagatc ggcgggcaga accccgccta catcgccaac
     4921 gccctcaagg cctaccgcgc gggcgagcgc tggcatccct cgatgcgcgg cattgcggcc
     4981 agcctgagcg acaaggacat cgccgacatc gccgcctact acgggggcag caaatgaccc
     5041 ggctcctttt cgccatgctc gcgctggcgc tcgccggcgc cgcgcaggcc ggcggcaacg
     5101 ccgcggccgg caaggacaag gcggcgcaga tctgcgccgc ctgccacggc cccagcggcg
     5161 ccaagccgac cgcgcccgag aatccgatcc tcgccggcca gcactacgac tacctggtga
     5221 aggcgctgca ggactacaag tccggcaagc gcaacaacgc catcatgaag ggcttcgccg
     5281 cctcgctgtc caagcaggac atcgaggacc tttcggcgtg gtttgcgagc caggcgtcgg
     5341 ggctgcacta ccagcgctag gcgcgcgtct tttcgcggaa gccgcacaga tcgcggatcg
     5401 cgcactgcgg gcagccaggc ttgcgcgcaa ggcacacgta gcgcccgtgc aagatgagcc
     5461 agtggtgcgc gtggcgccgg aattgcgccg gcacggcctg cagcagcttc aactccaccg
     5521 cctcgggcgt tttccccggc gcgagcccgg tgcggtttgc gacgcggaag atgtgcgtgt
     5581 cgacggcgat ggtgggctcg ccgaaggcca cgttcagcac cacgttggcg gtcttgcggc
     5641 cgacgccggg cagcgcctgg agcgcctcgc gcgaggcggg cacctcgccg ccgtggcgtt
     5701 cgaccagcag ccgcgacagc gcgacgacgt tcttcgcctt ggtgcggaat aggccgatgg
     5761 tgcggatgag ctcggtcacg cgcgcttcgc ccagcgccac catggcctgc ggcgtggggg
     5821 cggcctcgaa cagcttcggt gtcgcgagat tgaccgactt gtcggtcgcc tgcgccgaga
     5881 gcaccaccgc gaccagcagc tcgaacggcg tgcggtactg cagctcgctc ttcggctccg
     5941 ggtcggccgc gcgcaggcgc cggaacagct cgtgacgctt ggccggcgtc atcggcgctt
     6001 cgccagcgcg gcggcaagga tggcgcgtcg gcgctgcagg tcgctcgccg gcacctcggc
     6061 ctgacgcgca aggcggcgct tgcgcgctgc cgcgcgctgc ttggcggcgc gcgcgtcggc
     6121 cgccgtccag ggcgcgttcg ccggcaccac gtcgatgcag tccacggggc agggcggcag
     6181 gcacaggtcg cagccgatgc acagctccgc caccacggta tgcatgaagc cgcgcgcgcc
     6241 gacgatggcg tccaccgggc aggcgtcgat gcagcgcgcg cagccgatgc agcgcgcttc
     6301 gtcgatccgg gcgacggtgg cgcgcttcat cggccggtgt cgcgcgcgag ctgcgcgtgc
     6361 acgaccttgc catcgcgcac ctgcaggccg gtgtccagat cggc
//
LOCUS       DBNHKE010000144        15190 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_24431, whole genome shotgun sequence.
ACCESSION   DBNHKE010000144 DBNHKE010000000
VERSION     DBNHKE010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_24431"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="AC16O9_09725"
     CDS             complement(<1..1089)
                     /locus_tag="AC16O9_09725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR6516935.1"
                     /translation="MKHALVLKKGREKSLKRRHPWIFSGAIDHAAGKPAAGDTVEVRS
                     AEGAVLALAAYSPESQIRARVWSFDANAAIDAQFFKARVGEALARRAGLAAAKHTNAL
                     RLVHGESDGLPGVIVDRYDDVLVAQFLSAGAERWREALLDALAEATACEAIFERSDAE
                     VRKLEGLAPRVGFTRGNHEARRCPIIEYGLNFRVDVEQGQKTGFFLDQRENRQRVRAL
                     AAGREVLDGFSYTGGFAIAALAGGARRVLAVESSAPALEVARDNLAANALDASKVEFV
                     KADVFAHLRLLRDQGARFGLVVLDPPKFAPTAAQARNAARAYKDVNLLAFKLLAPGGL
                     LATFSCSGGVPAELFQSIVAGAALDAGAE"
     gene            complement(1086..1976)
                     /locus_tag="AC16O9_09730"
                     /pseudo
     CDS             complement(1086..1976)
                     /locus_tag="AC16O9_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
     gene            complement(1992..2636)
                     /locus_tag="AC16O9_09735"
     CDS             complement(1992..2636)
                     /locus_tag="AC16O9_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016243.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HIR6516936.1"
                     /translation="MTAEEVAQFFRTHPQFFDQHPELLESIYVPHPYGGRAIPLSERQ
                     ILSLRDKVKLLEAKLGELLKFGEDNDAISEKVHRLAAGLLGARDFAAASQALQFHLRE
                     DFAVPHVALRIWGRVLPVGVPEAAPVDDEMRVRVEAMGAPLCGPAAGSPFLAWFGDAA
                     EHVRSLALVPLGQTKAVGLLALGSEDAQRFYAEMGTLYLRRIGELTAAALAARA"
     gene            complement(2658..3437)
                     /gene="dapF"
                     /locus_tag="AC16O9_09740"
     CDS             complement(2658..3437)
                     /gene="dapF"
                     /locus_tag="AC16O9_09740"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6516937.1"
                     /translation="MQGAGNDFVVVDATRAPFTLGREQVRRLADRHLGVGCDQVLVVE
                     QARDPANDFGYRIFNADGGEVEQCGNGARCFVKFVHAKGLTKKKAIRVETPAGVIVPR
                     LEADGEVSVDMGAPARESPLVQPLEVGGARVELALLSIGNPHAVQLVADADAAPVATQ
                     GPLIEHHARFPGRVNAGYMQVVDRHTLKLRVWERGAGETLSCGTGACAAAVAGIARGL
                     VDSPVRVQTRGGALTIAWAGGDNRVSMKGPAQAVFEGSIEL"
     gene            complement(3452..4546)
                     /locus_tag="AC16O9_09745"
     CDS             complement(3452..4546)
                     /locus_tag="AC16O9_09745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6516938.1"
                     /translation="MKVPILDLAAEQAEIGAGIAAAMQRVLASGHFILGPEGAALEKE
                     IAALVGVPHAVACNSGTDALHLALRAAGIGPGDEVVTPAFTFIATAEAIAYVGATPVF
                     ADIDPATFNLTAATLERALTPGTRAVIVVHLFGQCAEMPAIAELCAKRGITLVEDAAQ
                     AIGADYAGTQAGAWGEFGCFSFYPTKNLGAYGDAGLVTAREARHDARLRMLRHHGSRQ
                     TYLHEEIGYNSRLDELQAAVLRVKLPHLARFTARRQQHAARYRELLAGANLQLPAVHG
                     RGAHVYHQFTVRVGNRDAVRKALTEAGVASGVYYPIPLHRQPVYRKQFGGLSLPASEA
                     AALEALSLPIYPQLADEQIRHVCATLAACL"
     gene            complement(4543..5499)
                     /locus_tag="AC16O9_09750"
     CDS             complement(4543..5499)
                     /locus_tag="AC16O9_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6516939.1"
                     /translation="MTKKTLRAAVVGVGYLGQFHAEKLAAAPGVELAAVVDADPARAK
                     NIAAKHGCRALTDPGALAGEVDLVSIAVPTEHHHAAALPFLEAGVHVLVEKPIATTLA
                     EADALIAAAKKSRALLAVGHLQRFNPAFRALAAAFAKPLFIDCERLSGFKQRGIDVDV
                     VLDLMIHDLDLALSLAGSEVAQVSACGFQVLTDSIDIANVRIEFADGCVANLSASRVS
                     QQPVRKLRVFQHDRYASADLQAGKLRIARRDAKQGGIVEDASAYDDRDELRAEIANFV
                     DAVRGGRPPLVAGEDGRRALALALEVGRLVRVRLERFRAVRA"
     gene            complement(5513..6382)
                     /locus_tag="AC16O9_09755"
     CDS             complement(5513..6382)
                     /locus_tag="AC16O9_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR6516940.1"
                     /translation="MSRLVFALMWLIHFLPLGAQAAFGNALGSLLYWLIPERRRVTRV
                     NLARCFPHMDPAARERLARAHFRAFTRSFVEHGLLWWAPRERIRRLIRLEGLEQLKAL
                     GDTPAILFVPHFVGLDAGGTRVTSEVDIVAMFARQKDPLFTRLLRHGRTRFGNQRMVS
                     RQEGVRAAIQAMRREKRPFYYLPDLDHGPRNAIFVPFFGVPAATVPGLSRIAKLAGAK
                     VLPCVTRMLPGGQGYVARIEAPWQDFPTDDVEADTRRMNAYIERVALEMPEQYLWMHK
                     RFKTRPPGEARLY"
     gene            complement(6402..7226)
                     /locus_tag="AC16O9_09760"
     CDS             complement(6402..7226)
                     /locus_tag="AC16O9_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6516941.1"
                     /translation="MMPLLARLPLRVLHAMGSVLGWGMYLLSPAYRARLRANLAQAGF
                     ADAATRRAAIAQAGRMVAEAPKLWLRPRDEVMALVRRVEGLQAAAAARKAGKGVVFLT
                     PHYGCFEITAHVVAEVGPLTALYRPARNERLERMIQAGRARGNVTLAAATLGGVRQLL
                     AALKRGEAVGILPDQVPAEGEGEWVEFFGRPAYTMTLAGKLAARADVAAFLVMGRRLP
                     GGEGYEVRVTPLAPARAGEPPARRVNRALEEAIGAHPAQYLWGYNRYKRPPGAPAP"
     gene            7269..8426
                     /gene="metK"
                     /locus_tag="AC16O9_09765"
     CDS             7269..8426
                     /gene="metK"
                     /locus_tag="AC16O9_09765"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR6516942.1"
                     /translation="MSDYLFTSESVSEGHPDKVADQISDAVLDAILSQDTAARVAAET
                     LVKDNLVVLAGEITKKREAHVDYQEIARNTIKRIGYTDPEIGFDARGCTVVVAYGEQS
                     PHIAQGVNRKVDMDQGAGDQGLMFGYACDETPSLMPLAIYLSHRLVERQAELRRDGRM
                     AWLRPDAKSQVTVKYVNGKPDSIDTVVLSTQHAPSMSGKALEEAVIEQIIKPVLPKNY
                     VKGKINFLVNPTGAFEIGGPKGDCGLTGRKIIVDTYGGSAPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAGLATKCLVQVSYAIGVAKPTSVMVTTEGTGKISDDRLSALVLA
                     NFDLRPRGIIQMLDLLRPVYEKTAAYGHFGREEPEFTWEATDKALSLKQAA"
     gene            complement(8567..9883)
                     /locus_tag="AC16O9_09770"
     CDS             complement(8567..9883)
                     /locus_tag="AC16O9_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR6516943.1"
                     /translation="MSLIRAAMDDPALLLYAVPLLLLFLLYYVRRARRHTRNVDRLQE
                     SVEAGLTEPVSLHPVFDPNRCIGSSACVTACPESAIGVIHGKAQLVNPASCIGHGACA
                     ASCPFDAIQLVFGTERRGIDIPNVKPNFESNVPGIFIAGELGGMGLIRKAVEQGRQAI
                     TTIKGMANGKAELDAVIVGAGPAGIAAGLGAIEHKLKYVVLEQEGSLGGAVFQYPRNK
                     IAMTAPVRLPLVGKMKFNEVSKEALLGFWQAVVKKTGLRIRFNERMEAIERKGGGFVV
                     KTTRGTYSTRAVVLAVGRRGSPRKLGVPGEELPKVVYRLIDAEQYRNQRVLVVGGGDS
                     ALEAALSIAAERGAKVALSYRGAAFGRVKPKNRERLEAAAAARRLQVLLGSEVEKIGA
                     REVVLRVGEKRLSLPNDAVIVCAGGVLPIDLLKKVGIRFETKHGTA"
     gene            10213..11133
                     /locus_tag="AC16O9_09775"
     CDS             10213..11133
                     /locus_tag="AC16O9_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516944.1"
                     /translation="MSELVAPRVASATPRTASAAARPARDLRVQSGRERMFLAGALAL
                     VALAWLVSRYGDYKSGSPLGYNLGIAGGVGLLVILLYPLRKRVRFLSGLGQTKSWFAL
                     HMTCGILAPFLILLHSKFHIGSINAGVSLVCMLLVATSGFIGRFIYVRIHHGLYGTRM
                     TLAELQAQVGFNSGEVRSKLSFVPRVEQWLREYEAAAIGRPKNVVQGLWRFLTLGARA
                     RWVRRRCTREFARVYRMHARAFKWDAARQRRHLAGGTELIADFVAGAQRVAQFSRYER
                     LFSLWHVLHVPLLWMMVLSAVAHVVAVHAY"
     gene            11208..12983
                     /locus_tag="AC16O9_09780"
     CDS             11208..12983
                     /locus_tag="AC16O9_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6516945.1"
                     /translation="MRRLLAACCALASSAAMAQTLESVLSPGPVIQGHAKVENECGKC
                     HVRFNRDAQDGRCLDCHKQTAADVRAHVGLHGRLKPQPCRECHTDHRGRDAKIAPLDE
                     RTFDHGQTDFGLRGAHADPKLKCSSCHLPGKKFREAPGRCNDCHAKKDVHKGRLGTAC
                     ADCHGERDWKVPQFDHDKTRFRLRGKHEPLKCAKCHKNDNFKNAPVTCIGCHKKDDKH
                     KARYGEKCQSCHNDRDWKTIDFDHDKDTRYALRGRHRATKCDACHTGHLYLDKLQTGC
                     NACHRKDDKHKGTLGVACGDCHSERDWKVGQFDHQKTRFPLRGKHDAIKCEACHKSAV
                     FKDAPTTCIGCHKKDDKHKGALGEPCGDCHTERNWKESKFDHAKTKFPLLQRHAKVKC
                     EACHRDPDYKRTPKDCYGCHKKDDKHEGQVGTKCENCHDASDWKKAKFDHARSRFALL
                     GAHLVVPCKKCHETPRYKDAKRDCNGCHERDDVHKRRLGPACEACHNARSWKSWDFNH
                     DRQTKYPLDGAHLKLSCYACHRQPVQKKATLPTSCVSCHAGEDVHDGTYGNQCEKCHV
                     TSSFKLIRQKLGVRGMADLLAARRE"
     gene            12988..14673
                     /locus_tag="AC16O9_09785"
     CDS             12988..14673
                     /locus_tag="AC16O9_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516946.1"
                     /translation="MRTTLRCAISVVMASVALLAASPARAQGSSAFDHLGTGFPLTGA
                     HQAARCESCHVRGVFRGTPRSCASCHTTNSRFSAVAMPATHIPVTQSCDACHNTVAFA
                     GARFNHAGVRPGTCASCHNNRQAPGKPAGHVVTTASCDACHRTTSYTPAFFNHANVAA
                     GTCLSCHARTAKPSTHVVTSASCDACHRTTAWLPATFSHAGVAAGTCASCHAKTAKPS
                     THVVTSASCDSCHRTTAWLPATMNHANVQAGGCLSCHASTAKPSTHVQSSASCDSCHR
                     TTAWLPATMNHAGIAAGTCLSCHARTPKPSTHVVSSASCDTCHRTTAWLPATMSHVGI
                     AAGTCLTCHASTPKPSTHVVTSASCDSCHRTTAWLPATFSHVGVAVGSCLTCHAGTPK
                     PSTHVVTSVSCDACHRTTAWLPATFSHTGVAAGTCLSCHAGTVKSTTHMQTSRSCDAC
                     HTTTAWLPPRTYTHLSPFYKPHNSGVTCRSCHSSNTEAATWTFAAYKPDCAGCHASRY
                     KASAHKKVDSPQILYTVAELKNCAGSCHTYTNSTFTTISRSRSGEHRSTAGGF"
     gene            14707..>15190
                     /locus_tag="AC16O9_09790"
     CDS             14707..>15190
                     /locus_tag="AC16O9_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516947.1"
                     /translation="MRWRALTGNTALLLCLLPAALLPSAARAEVLDELAVFPDRNDAV
                     IRITFSGPVQYLRHEVFGDALVELYFRPLTAEPTPATEARRVPPAASFPGVEVVYPQQ
                     PRSQTRKLTLRLSSPLKLRVRPADDHAIDFVVAGAAARLAPAGEPPAVPVARPAPVPL
                     P"
BASE COUNT         2352 a         5553 c         5165 g         2120 t
ORIGIN      
        1 ctcggcgccc gcgtcgagcg cggcgccggc gacgatcgac tggaagagct ccgccggcac
       61 gccgcccgag caggagaagg tggcgagcag tcccccgggc gcgagcagct tgaaggcaag
      121 caggttcacg tccttgtagg cgcgcgcggc gtttcgcgcc tgcgccgcgg tgggcgcgaa
      181 cttgggcggg tcgaggacca ccaggccgaa cctcgccccc tggtcgcgca agaggcgcag
      241 gtgcgcgaac acgtccgctt tcacgaactc gaccttcgac gcatcgagcg cgttggcggc
      301 gagattgtcg cgcgccacct ccagtgccgg cgccgagctc tccaccgcga gcacgcggcg
      361 cgcgccgccg gcgagcgcgg cgatggcgaa gccgcccgtg taggaaaagc catccagcac
      421 ctcgcgcccc gcggcgagcg cgcgcacccg ctgacggttc tcgcgctggt cgagaaagaa
      481 cccggtcttc tgcccttgtt ccacgtcgac gcggaaattc agcccgtact cgatgatcgg
      541 gcagcggcgc gcttcgtggt tgccccgcgt gaagcccacg cgcggcgcga ggccctcgag
      601 ctttcgcacc tcggcgtccg agcgctcgaa tatcgcctcg caggcggtgg cctcggcgag
      661 cgcatcgagc agcgcctcgc gccagcgctc ggcgcccgcc gacaggaact gcgccaccag
      721 cacgtcgtcg tagcggtcca cgatcacgcc gggcaggccg tcggactcgc cgtggacgag
      781 ccgcagcgcg ttggtgtgct tcgccgcggc aagcccggcg cggcgcgcga gcgcctcgcc
      841 tacgcgcgcc ttgaagaact gcgcgtcgat ggcggcgttc gcgtcgaagc tccacacgcg
      901 cgcgcggatc tgcgactcgg ggctgtacgc ggcgagcgca agcaccgcgc cctcggcgct
      961 cctcacctct accgtatcgc ccgcggcggg cttgccggcg gcgtggtcga tggcgccgga
     1021 gaagatccac ggatggcggc gcttgagcga tttctcgcgg cccttcttca ggacgagcgc
     1081 gtgcttcatt tcttcttcgc ccgcgggtgc gcggcgtcgt agacgcgcgc cagcgcctgg
     1141 tagtcgaggt gcgtgtagac ctgcgtggtg gcgatgctcg cgtgcccgag aagctcctgc
     1201 accgcgcgca ggtcctgcga ggactgcagc acgtgcgagg cgaaggagtg ccgcagcatg
     1261 tgcgggtgca gccttcccgg cacgccctgg cgcgcggcga gcttgcgcag ctcgcgctcg
     1321 accatgccgg gggcgatgcg acggccgcgc gcgccgacga agagcgcctt ctcgcccggc
     1381 gcggcgagct gcgcgcgcac gccgatccag gcctcgagcg cctgcctcgc gcgcgcgccc
     1441 accggcaccg tgcgggtctt cgcgcccttg ccggtgaccg tgacttcgcc ctcgcgcagg
     1501 tcgagccgcc cgtcgccgat gtcgagcgcg acgagctccg cgaggcgcag gcccgaggag
     1561 tacagcagct cgaacatggc gcggtcgcgc gccgccgcgg gcgtcgcgtc cgcgcccgcc
     1621 tcgagcaggc gctgcacctg ctcgaccgag agcgccttcg gcaggcgccg gccgggcttg
     1681 ggcgcgcgca cgccgtcgac cgggttggcg cggtagccct tgtggcgcac gagccagcga
     1741 tacagcccgc gccacgccga gagcaggctc gcgagcgacc ggggcgaaag accgcgcgcg
     1801 tgcagctgcg cgaccaggcg gcgcacctgc gccgggtcga ggctgtcgag gcgcgccgtg
     1861 ccggcgagcg cgagcagcgt gtccaggttg tggcggtaga tctcgaggct gcgccgcgcc
     1921 aggcgccgct ggtgctcgag cgcgccgagg tagtcgcgga cgaggaacgc gtccgggtgc
     1981 gtggcggcgt cctaggcccg cgcggcgagc gcggccgcgg tgagctcgcc gatgcggcgc
     2041 aggtagagcg tgcccatctc ggcgtagaag cgctgcgcgt cctcgctgcc gagcgcgagc
     2101 agccccacgg ccttggtctg cccgagcggc accagcgcga gcgagcgcac gtgctcggcg
     2161 gcgtcgccga accaggcgag gaacgggctc ccggccgccg ggccgcacag gggcgccccc
     2221 atcgcctcga cgcgcacgcg catctcgtcg tccaccggcg cggcctcggg cacgcccacc
     2281 ggcagcacgc ggccccagat gcgcagcgcc acgtgcggca cggcgaaatc ctcgcgcagg
     2341 tggaactgca gcgcctgcga ggcggcggcg aaatcgcgcg cgccgagcag gcccgcggcg
     2401 aggcgatgca ccttttcgct gatggcgtcg ttgtcctcgc cgaacttcag cagctcgccg
     2461 agcttggctt ccagcagctt caccttgtcg cgcagcgaca ggatctggcg ctccgagagc
     2521 gggatggcgc gcccgccgta cggatgcggc acgtagatcg actccagcag ctccgggtgc
     2581 tggtcgaaga actgcgggtg ggtgcggaaa aactgcgcga cttcttctgc ggtcatgagg
     2641 cgggtcgcct tgtcgtccta gagctcgatc gatccttcga acaccgcctg cgcggggccc
     2701 ttcatggaga cccgattgtc gccgcccgcc caagcgatgg tcaacgctcc gccgcgcgtc
     2761 tggacgcgca ccggcgagtc gacgaggccg cgcgcgatgc ccgccaccgc cgcggcgcag
     2821 gcgccggtgc cgcaagacag cgtctcgccc gcgccgcgct cccagacgcg cagcttcagc
     2881 gtgtgccggt ccacgacctg catgtagccc gcgttcacgc gcccgggaaa gcgcgcgtgg
     2941 tgctcgatga gcgggccttg cgtcgccacc ggcgccgcgt cggcgtcggc gacgagctgc
     3001 accgcgtgcg ggttgccgat cgacagcagg gcgagctcga cgcgcgcgcc gcccacttcc
     3061 agcggctgca ccagcggcga ctcgcgcgcc ggcgcgccca tgtcgacgct cacctcgccg
     3121 tcggcttcca gccgcggcac gatgaccccg gcaggcgtct ccacgcggat cgccttcttc
     3181 ttcgtcaggc ccttcgcgtg cacgaacttc acgaagcagc gcgcgccgtt gccgcattgc
     3241 tcgacctcgc cgccgtcggc gttgaagatg cggtagccga agtcattcgc ggggtcgcgc
     3301 gcttgttcca cgaccagcac ctggtcgcag cccacgccga ggtgccggtc ggccaggcgc
     3361 cgcacctgct cgcggccgag cgtgaacggc gcgcgcgtgg cgtccaccac gacgaagtcg
     3421 ttgcccgcgc cctgcatctt ggtgaacgcg atcagaggca agccgcgagc gtcgcgcaca
     3481 cgtgccggat ctgctcgtcc gcgagctggg gatagatcgg gagcgagagc gcttccagcg
     3541 ccgcggcctc ggacgcgggc agcgagaggc cgccgaattg cttgcggtac accggctggc
     3601 ggtgcagcgg gatcgggtaa tagacgccgc tcgccacgcc cgcctcggtg agcgcctttc
     3661 tcaccgcgtc gcggttgccc acgcgcacgg tgaactggtg gtagacgtgc gcgccgcgcc
     3721 cgtgcacggc gggcagctgc aggttcgcgc cggcgaggag ctcgcggtag cgcgcggcgt
     3781 gctgctggcg ccgcgcggta aagcgcgcca ggtgcgggag cttcacgcgc agtaccgcgg
     3841 cctgcagctc gtcgaggcgg ctgttgtagc cgatctcttc gtgcaggtag gtctggcggc
     3901 tgccgtggtg ccgcagcatc ctgaggcgcg cgtcgtggcg cgcttcgcgc gcggtgacca
     3961 ggcccgcatc gccgtaggcg ccgaggttct tcgtcgggta gaacgagaag cagccgaact
     4021 cgccccaggc gcccgcctgc gtgcccgcgt aatccgcgcc gatcgcctgc gcggcgtctt
     4081 ccaccagcgt gatgccgcgc tttgcgcaca gctcggcgat cgccggcatc tccgcgcact
     4141 ggccaaagag gtgcaccacg atcaccgcgc gcgtgcccgg cgtgagggcg cgctcgagcg
     4201 tcgcggcggt gaggttgaag gtcgcggggt cgatgtcggc gaacacgggc gtcgcgccga
     4261 cgtaggcgat ggcttccgcc gtcgcgatga aagtgaaggc cggcgtcacc acctcgtcgc
     4321 ccgggccgat gcccgcggcg cgcagcgcga ggtgcagcgc gtcggtgccg gagttgcacg
     4381 ccaccgcgtg cggcacgccc accagcgcgg cgatctcctt ttccagcgcc gcgccctcgg
     4441 ggccgaggat gaagtggccc gaggcgagca cgcgctgcat cgccgcggcg atcccggcgc
     4501 cgatctcggc ctgctcggcc gccaggtcga ggatcgggac cttcatgccc gcaccgcccg
     4561 gaagcgctcg agccgcacgc gcaccaggcg cccgacttcc agcgccagcg cgagcgcgcg
     4621 gcgcccgtcc tcgccggcaa ccagcggcgg ccggccgccg cgcacggcgt cgacgaagtt
     4681 cgcgatctcg gcgcgcagct cgtcgcggtc gtcgtaggcc gacgcgtcct cgacgatgcc
     4741 gccctgcttg gcgtcgcggc gcgcgatacg cagcttgccc gcctgcaggt cggccgaagc
     4801 gtagcggtcg tgctggaaca cgcgcagctt cctcaccggc tgctggctca cccggctcgc
     4861 cgagaggttg gcgacgcagc cgtcggcgaa ctcgatcctc acgttggcga tgtcgatcga
     4921 gtcggtgagc acctggaagc cgcaggccga gacctgcgcc acctcgctgc cggcgagcga
     4981 gagcgcgagg tcgaggtcgt ggatcatcag gtcgagcacc acgtcgacgt cgatgccgcg
     5041 ctgcttgaag cccgagagcc gctcgcagtc gatgaacagc ggcttggcga acgccgccgc
     5101 gagcgcgcgg aacgccgggt tgaagcgctg caggtgcccc accgcgagca gcgcgcgcga
     5161 tttctttgcc gcggcgatca gcgcatcggc ctcggcgagc gtggtggcga tcggcttttc
     5221 caccagcacg tgcacgcccg cttccaggaa cggcagcgcc gccgcgtggt gatgctcggt
     5281 cggcacggcg atcgacacga ggtcgacctc gccggcgagc gcgcccggat cggtgagcgc
     5341 gcggcagccg tgcttcgcgg cgatgttctt cgcgcgcgcg gggtcggcgt cgacgactgc
     5401 ggcgagctcg acgccgggcg cggcggcgag cttctcggcg tggaactggc cgaggtagcc
     5461 cacgcccacc accgccgcgc gcaaagtttt cttcgtcaca ggcgcattct agtcagtaca
     5521 agcgcgcctc gcccggcggg cgcgtcttga agcgcttgtg catccacagg tactgctcgg
     5581 gcatctcgag cgcgacgcgc tcgatgtacg cgttcatgcg ccgcgtgtcg gcctcgacgt
     5641 cgtcggtggg gaaatcctgc cagggcgcct cgatgcgcgc cacgtagccc tgcccgccgg
     5701 gcagcatgcg cgtcacgcag ggcagcacct ttgcgcccgc gagcttcgcg atgcgcgaca
     5761 ggcccgggac ggtggccgcg ggcacgccga agaacggcac gaagatggcg ttgcgcggcc
     5821 cgtggtcgag gtcgggcagg tagtagaacg ggcgcttctc gcggcgcatc gcctggatcg
     5881 cggcgcgcac gccctcctgg cgcgacacca tgcgctggtt gccgaacctc gtgcgcccgt
     5941 gccgcaggag gcgcgtgaac agcggatcct tctgccgcgc aaacatggcg acgatgtcga
     6001 cctcgctggt cacgcgcgtg ccgcccgcgt ccagcccgac gaagtgcggc acgaacagga
     6061 tcgccggcgt gtcgccgagc gccttcagct gctccaggcc ctcgagccgg atcaggcgcc
     6121 ggatgcgctc gcgcggcgcc caccacagca gcccgtgctc cacgaagctg cgcgtgaagg
     6181 cgcgaaagtg cgcgcgcgcc aggcgctcgc gcgccgccgg gtccatgtgc ggaaagcacc
     6241 tggcgaggtt gacgcgcgtc acgcgccggc gctcggggat cagccagtac aggagcgagc
     6301 cgagcgcgtt gccgaacgcc gcctgcgcgc cgagcggcag gaagtggatc agccacatca
     6361 gggcgaagac cagccggctc atgccggtgc caccgcgggg ctcacggcgc cggcgcgccc
     6421 ggcgggcgct tgtagcggtt gtatccccac agatactgcg ccgggtgcgc gccgatcgcc
     6481 tcctcgagcg cgcggttcac gcgccgcgcc ggcggctcgc ccgcgcgcgc gggcgcaagc
     6541 ggcgtcacgc gcacctcgta gccttcgccg cccggcaggc gccgtcccat gacgagaaac
     6601 gccgccacgt cggcgcgcgc cgcgagcttg cccgcgagcg tcatggtgta cgcgggccgg
     6661 ccgaagaact cgacccactc gccctcgccc tccgccggca cctggtccgg caggatgccc
     6721 accgcttcgc cgcgcttcag cgccgcgagc agctgccgca cgccgccgag cgtcgccgcc
     6781 gcgagcgtga cgttgccgcg cgcgcgcccg gcctgaatca tgcgctcgag gcgctcgttt
     6841 cgcgccggcc ggtagagcgc ggtgagcggg ccgacctcgg ccaccacgtg cgcggtgatc
     6901 tcgaagcagc cgtagtgcgg cgtgagaaag acgacgccct tgcctgcctt gcgcgccgcc
     6961 gccgcggcct gcagcccctc gacgcggcgc acgagcgcca tgacctcgtc gcgcgggcgc
     7021 agccagagct tgggcgcctc ggccaccatg cggccggctt gcgcgatcgc cgcgcggcgc
     7081 gtcgcggcgt cggcgaatcc cgcctgcgcg agattcgcgc gcaggcgcgc gcggtacgca
     7141 ggcgacagca ggtacatgcc ccagccaagg acgctcccca ttgcgtgcag cacgcgtagc
     7201 ggaagccgcg ccagcagggg catcatctga tatccttcaa gccttttttc ggagcgacgg
     7261 cgagagcgat gagcgattat ctttttactt ctgaatcggt gtccgagggc cacccggaca
     7321 aggtggcgga ccagatctcg gatgcggtcc tcgatgcgat tctaagccag gacacggcgg
     7381 cgcgagtcgc cgccgagacg ctggtgaagg acaacctggt ggtgctcgcc ggcgagatca
     7441 cgaaaaagcg cgaggcgcac gtcgactacc aggagatcgc gaggaacacg atcaagcgca
     7501 tcggctacac cgacccggag atcggcttcg acgccagagg ctgcaccgtg gtggtggcct
     7561 acggcgagca gtccccgcac atcgcgcagg gggtgaaccg caaggtcgac atggaccagg
     7621 gcgcgggcga ccagggcctg atgttcggct atgcctgcga cgagacgccc tcgctcatgc
     7681 cgctcgccat ctacctctcg caccgcctgg tcgagcgcca ggccgagctg cgccgcgacg
     7741 ggcgcatggc ctggctgcgg cccgacgcca agtcgcaggt cacggtgaag tacgtgaacg
     7801 gcaagcccga ttcgatcgac acggtggtgc tgtccacgca gcacgcgccc agcatgtcgg
     7861 gcaaggcgct ggaagaggcg gtcatcgagc agatcatcaa gccggtgctg ccgaagaact
     7921 acgtgaaggg caagatcaac ttcctcgtca acccgaccgg cgcgttcgag atcggcggac
     7981 cgaagggcga ctgcggcctc accggccgca agatcatcgt cgacacctac ggcggctcgg
     8041 cgccgcacgg cggcggcgcg ttctcgggca aggacccctc caaggtggac cgctcggcgg
     8101 cctacgccgc gcgctacgtg gcgaagaaca tcgtcgccgc gggcctcgcc accaagtgcc
     8161 tggtgcaggt gtcctacgcc atcggcgtgg ccaagcccac cagcgtcatg gtgaccaccg
     8221 aaggcaccgg caagatctcc gacgacaggc tttccgcgct ggtgctggcg aacttcgacc
     8281 tgcggccgcg cggcatcatc cagatgctgg atctgctgcg cccggtctac gagaagaccg
     8341 cggcctacgg ccacttcggg cgcgaggagc ccgagttcac ctgggaggcg acggacaagg
     8401 cgctgtcgct gaagcaggcg gcataagctc cgcgccggcg gcggcgagcc gtcggagcct
     8461 cgatcggttc tctatggtct cggcttttct ggcgggaaga ttcaccccgc cagaaaagcc
     8521 gagatgtatt taagggcaag aaacgaaggc accgcgtcgc cggcgcctac gccgtgccgt
     8581 gcttggtctc gaaccggatt ccgaccttct tcagcaggtc gatcggcagc acgccgcccg
     8641 cgcacacgat gaccgcgtcg ttcggcaggc ttaggcgttt ttcccccacg cgcagcacca
     8701 cctcgcgcgc gccgatcttt tctacctccg agccgagcag cacctgcagg cgccgcgcgg
     8761 ccgcggcggc ctcgaggcgc tcgcggttct tcggcttgac gcgcccgaac gccgcgccgc
     8821 ggtaggacaa cgcgactttg gcgccgcgct ccgccgcgat cgagagcgcc gcctcgagcg
     8881 cgctgtcgcc tccccccacc accagcacgc gctggttgcg gtactgctcg gcgtcgatca
     8941 gccggtagac caccttgggc agctcctcgc ccggcacgcc gagcttgcgc ggcgagccgc
     9001 gccggcccac cgccagcacc accgcgcgcg tcgagtaagt gccgcgcgtg gtcttcacca
     9061 cgaagccgcc gcccttgcgc tcgatcgctt ccatgcgctc gttgaagcgg atcctgaggc
     9121 cggtcttctt gaccaccgcc tgccagaagc cgagcagcgc ctctttcgac acttcgttga
     9181 acttcatctt gcccacgagc ggcaggcgca cgggcgcggt catcgcgatc ttgttccggg
     9241 gatactggaa caccgcgccg ccgagcgagc cttcctgctc gagcaccacg tacttcagct
     9301 tgtgctcgat ggcgcccagg cccgccgcga tgcccgccgg cccggcgccg acgatgaccg
     9361 cgtcgagctc ggccttgccg ttcgccatgc ccttgatggt ggtgatcgcc tgccggccct
     9421 gctcgacggc tttgcggatg aggcccatgc cgccgagctc gcccgcgatg aagatgccgg
     9481 gaacattgct ctcgaagttc ggctttacgt tggggatgtc gatgccgcgg cgctcggtgc
     9541 cgaacaccag ctggatcgcg tcgaacgggc acgaggcggc gcaggcgccg tgcccgatgc
     9601 acgaagcggg attcaccagc tgcgccttgc cgtggatcac gccgatcgcg ctttcggggc
     9661 acgccgtgac gcacgcgctc gagccgatgc agcggttggg atcgaacacc gggtgcagcg
     9721 acacgggctc ggtgagcccg gcttcgacgg actcctgcag gcgatcgaca ttgcgcgtgt
     9781 gcctgcgcgc gcgccgcacg tagtacagca gaaacaagag cagcagcggc accgcataga
     9841 gcagcagcgc ggggtcgtcc atcgccgctc tgatcagcga catggctcgc ccgcaccggt
     9901 cagtcgcggc gccaggtacg tgtcaagaac tgctctcatc ttatgccgac atcaagctcc
     9961 gaggtccatg tgaaaaatct ccgacctcat cgacagagcg cagcaatccg gtcgccgcct
    10021 agcgacactc tccaacgcac cgagtcgctg aatggcgcct accgttaatt actgcagggc
    10081 gcgctctgac cctttctgac atgatcagcg cgcacagctg gagcgtgcaa gttattccca
    10141 tgcagaacac aacatatatc acgctgactc cggccaagcg cgtccgcggt ccgggcgacg
    10201 aaggcgcgcg cagtgagtga gctggtcgcc ccccgcgtgg cttcagcgac gccgcgcacg
    10261 gcgagcgcgg cagcgcggcc cgcgcgagat ctgcgggtcc agtccgggcg cgagcggatg
    10321 tttctcgccg gcgccctggc gctcgtggcg ctcgcgtggc tggtaagccg ctacggcgac
    10381 tacaagtcgg gctcgccgct cggctacaac ctgggaatcg cgggcggcgt ggggctgctc
    10441 gtgatattgc tgtatccgct gcgcaagcgc gtgcgcttcc tgagcggcct gggccagacg
    10501 aagtcgtggt tcgcattgca catgacctgc ggcatcctgg cgccgtttct cattctcctg
    10561 cattccaagt tccacatcgg ctcgatcaac gcgggcgttt cgcttgtctg catgctgctg
    10621 gtcgccacca gcggcttcat cggccgcttc atctacgtgc gcatccacca cgggctgtac
    10681 ggcacgcgca tgacgctggc ggagctgcag gcgcaggtcg gattcaattc cggcgaagtg
    10741 cgctcgaagc tgagcttcgt gccgcgcgtc gagcaatggt tgcgggaata cgaggcggcg
    10801 gcgatcgggc ggccgaaaaa cgtcgtgcag ggcctgtggc gcttcctcac cctcggcgca
    10861 cgcgcgcgct gggtgcgccg ccgctgcacg cgcgagttcg cgcgcgtcta ccgcatgcac
    10921 gcgcgcgcgt tcaaatggga cgccgcgcgg cagcggcgcc acctcgccgg gggcaccgag
    10981 ctcattgcgg acttcgttgc cggcgcgcag cgcgtggcgc agttcagccg ctacgagcgc
    11041 ctgttctccc tgtggcacgt tctgcatgta ccgctcctgt ggatgatggt gttgagcgcc
    11101 gtcgcccacg tggtcgcggt gcacgcctac taaccgacag gccacgccgc tgagtgcccc
    11161 ccgcctctcc gtcatgccct gcgcgcgccc gctgtcatcg tccatggatg cgccgtctcc
    11221 tggcggcgtg ctgcgcgctc gcctcgagcg cggccatggc ccagacgctg gagagcgtgc
    11281 tctcgcccgg cccggtgatc caggggcatg ccaaggtcga gaacgagtgc ggcaagtgcc
    11341 atgtgcgctt caaccgcgac gcgcaggacg ggcggtgcct cgactgccac aagcaaaccg
    11401 ccgccgacgt gcgcgcgcac gtgggcctgc acggccgcct gaagccgcag ccctgccgcg
    11461 agtgccacac ggaccaccgg ggccgcgacg cgaagatcgc cccgctcgac gagcgcacgt
    11521 tcgaccacgg ccagaccgac ttcgggctgc gcggcgcgca tgcggacccg aaattgaagt
    11581 gcagcagctg ccacttgccc gggaagaagt tccgcgaagc gcccgggcgc tgcaacgact
    11641 gccacgccaa gaaggacgtg cacaaggggc gcctcggcac ggcctgcgcc gactgccacg
    11701 gcgagcgcga ctggaaggtg ccgcaattcg atcacgacaa gacgcgcttt cgcctgcgcg
    11761 gcaagcacga gccgctgaag tgcgcgaagt gccacaagaa cgacaacttc aagaacgcgc
    11821 cggtcacctg cattggctgc cacaagaagg acgacaagca caaggcgcgc tacggcgaga
    11881 agtgccagag ctgccacaac gatcgcgact ggaagacgat cgacttcgac cacgacaagg
    11941 acacgcgcta cgcgctgcgc gggcggcacc gcgcgacgaa atgcgacgcc tgccacaccg
    12001 ggcacctgta cctcgacaag ctgcagacgg gctgcaatgc ctgtcaccgc aaggacgaca
    12061 agcacaaggg cacgctcggc gtggcctgcg gcgactgcca cagcgagcgc gactggaaag
    12121 tcgggcagtt cgaccaccag aagacgcgct ttccgctgcg cggcaagcac gacgccatca
    12181 agtgcgaggc ctgccacaag tcggccgtgt tcaaggacgc gcccaccacc tgcatcggct
    12241 gccacaagaa ggacgacaag cacaagggcg cgctcggcga gccgtgcggc gattgccaca
    12301 ccgagcgcaa ctggaaggaa agcaagttcg accacgcgaa gaccaagttc ccgctgctgc
    12361 agcgccacgc caaagtgaag tgcgaagcct gccaccgcga ccccgactac aagcgcacgc
    12421 ccaaggactg ctacggctgc cacaagaagg acgacaagca cgagggccag gtgggcacca
    12481 agtgcgagaa ctgccatgac gccagcgact ggaagaaggc gaaattcgac catgcccgct
    12541 cgcgcttcgc gctgctcggc gcgcacctcg tggtgccgtg caagaagtgc cacgaaaccc
    12601 cgcgctacaa ggacgccaaa cgcgattgca acggctgcca cgagcgcgac gacgtgcaca
    12661 agcgccgcct cggcccggcc tgcgaggcct gccacaacgc gcgctcatgg aagagctggg
    12721 acttcaacca cgatcgccag accaagtacc ccctggatgg cgcgcacctc aagctcagct
    12781 gctacgcctg ccaccgccag ccggtgcaga agaaggccac gctgcccacg agctgcgtca
    12841 gttgccacgc cggcgaggac gtgcacgacg gcacctacgg caaccagtgc gagaaatgcc
    12901 acgtgacctc atcgttcaag ctcattcgcc agaagcttgg cgtgcgcggg atggccgacc
    12961 tgctggccgc ccgccgggag taatgccatg agaacgaccc tgcgctgtgc gatttccgtg
    13021 gtgatggcga gcgtcgccct gctcgccgcg agtcccgcgc gcgcgcaggg ctcgagcgcg
    13081 ttcgatcacc tcggcaccgg cttcccgctc accggcgcgc accaggccgc gcgttgcgag
    13141 tcgtgccacg tgcgcggcgt cttcaggggc acgccgcgaa gctgcgcttc gtgccacacc
    13201 acgaacagcc gcttctcggc cgtcgccatg ccggcgacgc acattccggt gacgcagtcc
    13261 tgcgacgcct gtcacaacac cgtggccttc gccggcgcgc gcttcaacca cgccggggtg
    13321 cgtcccggca cctgcgcgag ctgccacaac aaccggcagg cgcccggcaa gccggccggc
    13381 cacgtcgtca cgaccgcctc ctgcgacgcc tgtcatcgca cgacgtccta cacacctgcg
    13441 ttcttcaatc acgccaacgt cgccgccggc acctgcctga gctgccacgc caggaccgcc
    13501 aagccctcga cccacgtggt gacgagcgcc tcctgcgacg cttgccatcg caccaccgcc
    13561 tggctgccgg cgactttcag ccacgccggc gtcgccgccg gcacctgcgc gagctgccac
    13621 gccaagacgg ccaagccctc gacccacgtg gtgacgagcg cctcctgcga ctcttgccat
    13681 cgcaccaccg cgtggctgcc ggcgaccatg aaccacgcca acgtgcaggc cggcgggtgc
    13741 ctgagctgcc acgccagcac cgcgaaaccc tcgacgcacg tgcagtccag cgcgtcgtgc
    13801 gacagctgcc accgcaccac cgcctggctg cccgcgacga tgaaccacgc cggcattgcg
    13861 gcgggcacct gcctgagctg ccacgccagg acacccaagc cctcgaccca cgtggtttcg
    13921 agcgcctcct gcgacacctg ccatcggacc accgcgtggc tcccagcgac catgagccac
    13981 gtcggcatcg ccgccggcac ctgcctcacc tgccacgcca gcacacccaa gccctcgacc
    14041 cacgtggtga cgagcgcctc ctgcgactct tgccaccgca cgaccgcctg gctgccggcg
    14101 accttcagcc acgtcggcgt cgcggtcggc agctgcctca cgtgccacgc cggcacgccc
    14161 aagccctcga cccacgtggt gacgagcgtc tcctgcgacg cgtgccatcg caccaccgcg
    14221 tggctcccgg caacgttcag ccacacgggc gttgccgcgg gcacctgcct gagttgccac
    14281 gccggcaccg tcaagtcgac gacgcacatg cagaccagca ggtcctgcga cgcctgccac
    14341 acgaccaccg cctggctgcc gccgcgcacg tacacgcatc tgtcgccctt ctacaagccg
    14401 cacaattcgg gggtgacctg ccggagctgc cactcgtcga acaccgaggc ggccacctgg
    14461 acgttcgccg cgtacaagcc ggactgcgcg ggttgtcacg cctcgcgcta caaggcgagc
    14521 gcgcacaaga aggtggactc cccgcagatc ctgtacacgg tggccgagct gaagaattgc
    14581 gcgggcagct gccataccta caccaacagc actttcacca cgatctcgag atccagatcc
    14641 ggagaacacc ggtccaccgc tggcggcttc tgatccgatg tcgaacaaga ccaaagaatc
    14701 gaccggatgc gctggcgagc actaaccggg aacaccgcgc ttctcctgtg cctgcttccc
    14761 gccgcgctgc tgccgagcgc agcgcgcgcg gaagtgctcg acgagctcgc ggtcttcccg
    14821 gaccgcaacg atgcggtgat ccgcatcacg ttttccgggc ccgtgcagta cctgcggcac
    14881 gaagtcttcg gcgatgcgct cgtcgagctc tacttccggc cgctgacggc tgaaccgacg
    14941 cccgccacgg aagcgcgccg ggtgccgccc gccgcatcgt tcccgggcgt cgaggtggtc
    15001 tacccgcagc agccgcgctc gcagacgcgc aagctgaccc tgcgcctgag cagccccctc
    15061 aagctgcgcg tgcgtccggc cgacgatcac gccatcgact tcgtcgtcgc gggcgccgcc
    15121 gccaggctcg cgcccgcggg cgagccgccg gcggtgccgg tggcgcggcc cgcgcccgtg
    15181 ccgctgccgc
//
LOCUS       DBNHKE010000145         4874 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1082231, whole genome shotgun sequence.
ACCESSION   DBNHKE010000145 DBNHKE010000000
VERSION     DBNHKE010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1082231"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="AC16O9_09795"
     CDS             <1..1160
                     /locus_tag="AC16O9_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516948.1"
                     /translation="KLSYALSWSAGGGDVFAFHVVNLAAHAANVLLAWQILRTLFERM
                     GVGGEGFAAFAAALLFAVHPAHTEAVTYVSGRSTSMMALFYLASVLAYLRRAPRGVSP
                     ALFLAALATKEVAVTLPFALLLCEALDLRHRFEWRMVLRRQVLHWGVLGLGLLAMAAL
                     PRYREMLEASLALRSLADQLALQTGALVRHVGVLILAIPPNIDPEIVPAPLVAGLVIL
                     AAIAAGFALLRAQPWVALALLWFFLHLAPTNSFLPRLDAVNDRQLYLASIGPLALAGI
                     AIALLPRGRSVLAAALALALGLGTVLRNQDYRSEVALWSDTVRQSPANARAFNNLGFA
                     LQQEGLRDEACAAYAEALRQDAEQLRAAVNYRELRCSPDLRSPGAAPETPR"
     gene            complement(1105..2256)
                     /locus_tag="AC16O9_09800"
     CDS             complement(1105..2256)
                     /locus_tag="AC16O9_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6516949.1"
                     /translation="MPADLKIAEIKAYPTSFPVPPENRVALGIGTAVKRDAVVVKVRT
                     EGGLVGWGESHHGRAHTAVAKLIETTLAQLVLGMDASDVVGVWKRIYDKQLASHGMGA
                     GTCLAMSGIDCALWDIRGQAAGMPLYQLLGGAKKGIPAYAGGVSLGFQAPGPLIDELR
                     RNLALGYKAVKLRVGDAVKRDIGRIRAVRQACGEDLVILADANIGYSVEDVRRVMPAL
                     DELKVGWLEEPFPAHDYRSYAIAKGFGRTPLAAGENHYTRFEFNRVIEDGAITILQPD
                     LSKTGGITEALRIAAMASAYKLPIHPHSSMTGLNHAASIHFLCAIDNGGYFEGDVSRA
                     NKFRDELVSKPYEVDKDGNVWPLDKPGLGLEVNEAFLEQHPAIEGPGYI"
     gene            complement(2290..3069)
                     /locus_tag="AC16O9_09805"
     CDS             complement(2290..3069)
                     /locus_tag="AC16O9_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR6516950.1"
                     /translation="MTDLSHVNIPSLRSRVSAEEWQARVDLAACYRLIAHYGMNDLIY
                     NHATARVPGEEGHFLINAYGYAYEEVTASSLVKIDFDGKVVLDSGTGYGINQAGFVIH
                     SAIHRGRKDVACVIHTHSPAGMAVSALKCGLLPLTQNAMFFGEIAYHDYEGPAIDLDE
                     QQRLVKDLGAASAMILRNHGLLSAGRTVCEAFVVLHWLERACQAQVMAMACNTELNHA
                     GADVVKLTNERYAPGKRRKITELEWPALLRMLDRRDPSFRE"
     gene            complement(3066..4220)
                     /locus_tag="AC16O9_09810"
     CDS             complement(3066..4220)
                     /locus_tag="AC16O9_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HIR6516951.1"
                     /translation="MRGSVAIAGVGLAGCGEAPGWSEMEIAAAAAKNALDDAGLTARE
                     VDGIVIASTNIFMSGLALAEHLGVRPKFTESSMLGGSSFVGHLIPAMLALDAGLCDTI
                     LVAYGSNQRTGTGRAEAGRHKMALDPQVFEHPYKPFNPPTSYALAAARHMHQYGTTRA
                     QLAEIAVAARQWAQRNPEAFARDPLTLEDVLDSRMISDPLTVRDCCLVTDGGGAYVLT
                     RAERARALKKAPAYLLGVGAAHWHRQISSMPDLTVTAATESGQRAFAMAGLAPQDVDV
                     VELYDAFTINTLLFLEDLGFCGKGEGGAFVSGGRIAPGGELPVNTNGGGLSCVHPGMY
                     GIFLLIEAVRQLRREAGDRQVRDAAVALAHGNGGVLSSQITAILGTQDTL"
     gene            complement(4217..4576)
                     /locus_tag="AC16O9_09815"
     CDS             complement(4217..4576)
                     /locus_tag="AC16O9_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6516952.1"
                     /translation="MSPPSGPEKRYLDALAGGRFEIQKCAACAKHVFYPRVVCPHCGA
                     GELGWVAPSGRGTVYSTTVVRRKPADGGDYNVCLVDLAEGVRMMSRVVGLPPEAVKIG
                     MAVKARLADGLVEFTPA"
     gene            complement(4554..>4874)
                     /locus_tag="AC16O9_09820"
     CDS             complement(4554..>4874)
                     /locus_tag="AC16O9_09820"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6516953.1"
                     /translation="AIHSMPRTVGTGLGIISSSGSIVARLSCHNRPRMPTFHVRCEVA
                     GRVVRIEARAGARVAAEDTLLLIECMKMEIPVPAPAAGVLETLHVAEGEAVEEGQHVA
                     TLRA"
BASE COUNT          676 a         1698 c         1691 g          809 t
ORIGIN      
        1 tcaagctgtc gtacgcgctg tcctggagcg cgggcggcgg cgacgtcttc gcctttcacg
       61 tcgtcaacct cgccgcgcac gcggcaaacg tgctgctcgc gtggcagatc ctgcgcacgc
      121 tcttcgagcg catgggcgtc ggcggcgagg gcttcgccgc ctttgccgcc gcgctgctct
      181 tcgccgtgca cccggcgcac acggaggcgg tgacctacgt ctcaggccgc tcgacgtcga
      241 tgatggcctt gttctatctg gcgagcgtcc tcgcctacct gcgccgcgca ccgcgcgggg
      301 tctcgcccgc gctgttcctc gccgcgctgg cgacgaagga ggtggccgtg acgctgccgt
      361 tcgcgctgct gctgtgcgag gcgctcgatc tgcgccaccg gttcgagtgg cgcatggtcc
      421 tgcggcgcca ggtcctgcac tggggcgtgc tcggcctggg gctgctggcg atggcggcgt
      481 tgccgcgcta ccgcgagatg ctggaggcaa gcctcgctct gcgctcgttg gccgaccagc
      541 tggcgctgca gactggcgct ctcgtccggc acgtcggcgt gctgatcctc gccatcccgc
      601 cgaacatcga ccccgagatc gttccggcgc cgctggtcgc gggcctggtg atcctcgccg
      661 cgatcgccgc gggattcgcg ctgctgcggg cgcagccgtg ggtggcgctg gcgctgctat
      721 ggttctttct gcacctcgcg cccaccaatt cgttcctgcc gcgcctcgac gcggtcaacg
      781 accgccagct ctacctcgcc tcgatcgggc cgctggcgct ggccggcatc gcgatagcgc
      841 tgttgccgcg agggcgatcc gtgctggccg cagcgctcgc cctggcgctt ggcctgggta
      901 cggttctgcg caaccaggac taccgctcgg aagtcgcgct gtggagcgac accgtgcgcc
      961 agtcgccggc aaacgcgcgc gccttcaaca acctcggttt cgcgctgcag caggaaggcc
     1021 tgcgcgacga ggcctgcgcg gcgtacgccg aggcgctgcg gcaggatgcc gaacagctgc
     1081 gcgcggccgt gaactaccgc gagctcagat gtagcccgga ccttcgatcg ccgggtgctg
     1141 ctccagaaac gcctcgttga cctcgagccc gagcccgggc ttgtccagcg gccagacgtt
     1201 gccgtccttg tcgacctcgt agggcttgga caccagctcg tcgcggaact tgttcgcgcg
     1261 cgacacgtcg ccctcgaagt agccgccgtt gtcgatcgcg cagaggaaat ggatcgacgc
     1321 ggcgtggttc agcccggtca tcgagctgtg cggatggatc ggcagcttgt acgccgaggc
     1381 catggccgcg atgcgcagcg cctcggtgat gccgccggtc ttcgacaggt cgggctgcag
     1441 gatggtgatc gcgccgtcct cgatcacgcg gttgaactcg aagcgcgtgt aatgattttc
     1501 gcccgcggcg agcggcgtgc gcccgaagcc cttggcaatc gcatagctgc ggtagtcgtg
     1561 cgcggggaag ggctcttcca gccagccgac cttgagctcg tccagcgccg gcatcacgcg
     1621 gcgcacgtcc tcgacgctgt agccgatgtt ggcgtcggcg aggatgacga ggtcctcgcc
     1681 gcaggcctgg cgcacggcgc ggatgcgccc gatgtcgcgc ttcacggcgt cgcccacccg
     1741 cagcttcacc gccttgtagc caagcgcgag gttgcggcgc agctcgtcga tcagcggccc
     1801 gggcgcctgg aaaccgagcg acacgccccc ggcgtaggcc gggatgcctt tcttcgcacc
     1861 gccaagcagc tggtagagcg gcatgcccgc cgcctggccg cggatgtccc ataaggcgca
     1921 gtcgatgccg ctcatggcaa ggcaggttcc cgcgcccatg ccgtggctgg cgagctgctt
     1981 gtcgtagatt ctcttccaca cgccgaccac gtcggatgcg tccatgccga ggacgagctg
     2041 cgcgagcgtg gtctcgatca gctttgccac cgcggtgtgt gcgcgcccgt ggtgcgactc
     2101 gccccagccc acgaggccgc cttcggttct caccttcacg accaccgcat cgcgcttgac
     2161 ggcggtgccg atgccgagcg ccacccggtt ctccggcggc accggaaacg acgtgggata
     2221 ggccttgatc tcagcgattt tcaggtcggc gggcatgccg ggccttcgga tgggcgggcg
     2281 cgagccgcgt cactcccgga aggaaggatc gcgccggtcg agcatgcgca gcagcgccgg
     2341 ccactcgagc tcggtgatct tgcggcgctt gccgggcgcg tagcgctcgt tggtcagctt
     2401 caccacgtcg gcgccggcgt ggttgagctc cgtgttgcag gccatcgcca tcacctgcgc
     2461 ctggcaggcg cgctcgagcc agtgcagcac cacgaacgcc tcgcacaccg tgcgcccggc
     2521 gctgagcagg ccgtggttcc tgaggatcat ggccgaggcc gcgccaaggt ccttcaccag
     2581 tcgctgctgc tcatcgaggt cgatcgccgg gccctcgtag tcgtgatatg cgatctcgcc
     2641 gaagaacatc gcgttctgcg tgagcgggag caaaccgcac ttcagcgccg acaccgccat
     2701 gccggcgggc gagtgcgtgt ggatcacgca cgccacgtcc ttgcggccgc ggtggatcgc
     2761 gctgtggatg acgaagccgg cctggttgat gccgtagccg gtgcccgagt cgagcaccac
     2821 cttgccgtcg aagtcgatct tcaccaggct cgaggcggtg acctcctcgt acgcgtagcc
     2881 gtaggcgttg atgagaaagt ggccttcctc gcccggcacg cgcgcggtgg cgtggttgta
     2941 gatcaggtcg ttcatgccgt agtgcgcgat cagccggtag caggcggcga ggtccacgcg
     3001 cgcctgccat tcctcggcgc tcaccctgct gcgcagggac gggatgttca cgtgcgagag
     3061 atcggtcata gcgtgtcctg ggttcccaat atcgcggtta tctggctgga gagcaccccg
     3121 ccgttgccgt gcgcgagggc gacggcggcg tcccgcacct gccggtcccc ggcctccctg
     3181 cgcaattgtc gcaccgcttc gatgagcagg aagatcccgt acatgccggg atgcacgcac
     3241 gacaggccgc cgccgttcgt gttcaccggg agctctccgc ccggcgcaat gcgcccgccc
     3301 gagacgaagg cgccgccttc gccctttccg cagaatccca ggtcctcgag aaagagcagg
     3361 gtattgatgg tgaaggcgtc gtagagctca acgacgtcga cgtcctgcgg cgcgaggccc
     3421 gccatcgcga aggcgcgctg cccggattcg gtcgccgcgg tcacggtgag gtcgggcatc
     3481 gaggagatct gccggtgcca gtgcgcggcg ccgacgccaa ggagatacgc aggggctttc
     3541 ttcagcgccc tggcgcgctc ggcgcgcgtc agcacgtaag cgccaccgcc atcagtcacc
     3601 aggcagcagt cgcgcacggt cagcggatcg ctgatcatgc gcgagtccag cacgtcctcg
     3661 agcgtgagtg gatcgcgcgc aaaggcttcg ggattccgct gcgcccattg ccgcgccgcc
     3721 accgcgatct cggcgagctg cgcgcgcgtc gtgccgtact ggtgcatgtg gcgtgccgcg
     3781 gcgagcgcgt aggaggtggg tggattgaac ggcttgtagg gatgctcgaa cacctgcggg
     3841 tcgagcgcca tcttgtggcg ccccgcctcc gcgcgcccgg tgccggtgcg ctggttcgag
     3901 ccgtaggcga cgaggatggt gtcgcacagg ccggcgtcga gcgcgagcat cgcggggatc
     3961 aggtggccga cgaacgacga gccgccgagc atgctcgact cggtgaactt cggccgcacg
     4021 ccgaggtgct cggcgagcgc cagccccgac atgaagatgt tggtgctggc gatgacgatg
     4081 ccgtcgacct cgcgcgcggt gaggccggcg tcgtcgagcg cgttcttcgc cgccgccgcc
     4141 gcgatctcca tctcgctcca gcccggcgcc tcgccgcagc cggcgagccc cacgcccgcg
     4201 atggcgaccg agcccctcat gccggcgtga attccacgag gccatcggcg aggcgcgcct
     4261 tgacggccat gccgatcttc acggcctcgg gcggcaggcc gaccacgcgg ctcatcatgc
     4321 gcacgccttc ggcgaggtcg acgaggcaca cgttgtagtc gccgccgtcg gcgggcttcc
     4381 tgcgcacgac agtggtcgaa tagacggtgc cgcggccgct cggcgccacc cagccgagct
     4441 cgcccgcgcc gcagtgcggg cagaccacgc gtgggtagaa cacgtgcttc gcgcaggcgg
     4501 cgcatttctg gatttcgaaa cggccgccgg cgagcgcgtc gagatagcgc ttctcaggcc
     4561 cggagggtgg cgacatgctg gccttcctcc acggcttcgc cttcggcgac gtgcagcgtc
     4621 tcgagcacgc cggcggcggg cgcgggcacc gggatctcca tcttcatgca ttcgatgagc
     4681 agcagcgtgt cctccgcggc cacgcgcgcg ccggcgcgcg cctcgatgcg caccaccctg
     4741 ccggccacct cgcagcgcac gtgaaacgtc ggcatgcgcg ggcgattatg gcaggacagg
     4801 cgtgcaacaa tcgagcccga actcgaaatg atccccagac ccgtcccgac ggtgcgcggc
     4861 atgctgtgga tggc
//
LOCUS       DBNHKE010000146         9077 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_183527, whole genome shotgun sequence.
ACCESSION   DBNHKE010000146 DBNHKE010000000
VERSION     DBNHKE010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_183527"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC16O9_09825"
     CDS             <1..391
                     /locus_tag="AC16O9_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013548.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR6516954.1"
                     /translation="LAYYYLGKFDIVAFGTGAILDQLETRALAVSGVYSAPLGHGYNF
                     IAKLGIAFTEAQYDCRTGCGVAPFIDTRRRGNSGLIGLGITSQVSSNVALRMEFEHIG
                     NVRHAMSNTVFFNDGYDMFSVGLHLNF"
     gene            621..824
                     /locus_tag="AC16O9_09830"
     CDS             621..824
                     /locus_tag="AC16O9_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6516955.1"
                     /translation="MSAGTVKWFNDAKGFGFITPEDGSKDLFVHFSAIQGGGFKTLKE
                     NDKVQFDVVQGPKGTQATNVRPA"
     gene            887..1117
                     /locus_tag="AC16O9_09835"
     CDS             887..1117
                     /locus_tag="AC16O9_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516956.1"
                     /translation="MDKRKRWREQEQQVVERWNAAEARYREVHAALQRLAGNGAEAEA
                     LKQEVERARADIEAVRRQVARLKVEFTSGKRY"
     gene            1118..1360
                     /locus_tag="AC16O9_09840"
     CDS             1118..1360
                     /locus_tag="AC16O9_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516957.1"
                     /translation="MNTRNNLTRGRAALWSRDKIELLSTPEVRNLHANAERLLETEIA
                     ALCLEVLGTRPRSGVAAPAKPAPRVRRAKAKAEQAA"
     gene            1389..2048
                     /locus_tag="AC16O9_09845"
     CDS             1389..2048
                     /locus_tag="AC16O9_09845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR6516958.1"
                     /translation="MCGRYVSPEQASIERAWKIDRASSNPFPRRFNVQPTTAVPMLRL
                     EDGARTLSGARWGLIPHWWKQDKPPSFSINARLEDISRKPMWRDPIRRSRCLVPAEGW
                     YEWQETPSGKQPHYITRRDRRPFCFAGLYAHWRDTLSCAILTTQALGPIAEVHERMPV
                     VLPEDAFGAWLEPAMHDAAVATTMAQAPMAPEDFEHYPVSRRVNNARNEGPDLIEPLR
                     A"
     gene            complement(2045..2215)
                     /locus_tag="AC16O9_09850"
     CDS             complement(2045..2215)
                     /locus_tag="AC16O9_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516959.1"
                     /translation="MPNLSPLKFCMHGQHSKPRAQFRVLPGGERRREVCAECYEKIMA
                     ARKERAAAARKR"
     gene            2309..3217
                     /locus_tag="AC16O9_09855"
     CDS             2309..3217
                     /locus_tag="AC16O9_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6516960.1"
                     /translation="MAVPLLAALVAGAGGAVQAFDLQGHRGTRGTAPENTLPGFAAAL
                     ATGVDTLELDTGVTRDGVVVIHHDRRLNPDIARGPDGRWIEAPGPLLLELDFKDLQRY
                     DVGRLRPGSDYAARFPEQTPADGARIPRLAELFALVRKAGNDQVRFNIETKISPAAPA
                     ETLPPEAFARALIAEIRAAGMAGRTTVQSFDWRTLQAVEREAPEIATAYLTGRRRGGS
                     QPQAVHAAGGRIWSPNYEELDSVALVEARRLGLKVVPWTMNEPGFIERFLDLGVDGII
                     TDYPERVRAELVRRGMPLPARTPVEP"
     gene            3220..3813
                     /locus_tag="AC16O9_09860"
     CDS             3220..3813
                     /locus_tag="AC16O9_09860"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6516961.1"
                     /translation="MPPVTGALLAANVLVFMLQLAGGLPLVEHFALWPPATSGPRAGP
                     PFELWQLLSYSFLHGSLLHLMFNMLALYTFGRDVERLFGSQWYLQFYFASVVAAALSH
                     LVVTGWMGAPPYPTVGASGGVYGVLLAYGIYFPRRTVLLLFPPIPMPARVFVVLFAAL
                     ELYFGVTGTASGIAHFAHLGGMLGGWLMIRSRRRRRG"
     gene            3836..4186
                     /locus_tag="AC16O9_09865"
     CDS             3836..4186
                     /locus_tag="AC16O9_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516962.1"
                     /translation="MLRELPGQQSRSRFMVGKYEVIAQPIPGSAHMLRYTVFLKGTRI
                     GAMASVPTESDCRFLENPPPVPPVKPFQVFYRPGRPKKGSQPASAADRALGSTPHEDL
                     PAGLAIPSLEKSAE"
     gene            4269..5627
                     /locus_tag="AC16O9_09870"
     CDS             4269..5627
                     /locus_tag="AC16O9_09870"
                     /inference="COORDINATES: protein motif:HMM:NF023165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR6516963.1"
                     /translation="MRKLVIALLAVLATASGALAEERAPYSQAELDQMLAPIALYPDA
                     LLSQVLMAATYPLEVVEAARWSRAHSDLKGEDAVRAVQDQDWDPSVKSLLAFPDLLTR
                     MDEELDWTRRLGDAFLEQEPHVMQTVQRLRRKAEAASQLGSGEHLRVLEEDGVIVIEQ
                     ANPRVVYVPYYDPLVVYGAWWWPAHPPVVWAPWSGYTVYRPGIWWGVGVGISSGFFYG
                     GVRWIDRRVVVVRPSPYDARPLATGPWRHNPAHRRGIVYRHPETQRRFAPVEPRLQRP
                     AVRQESQREERRAPPRAEPRWPPREERRAPQRDEPRWQPREERRGPQRDERRWQPREE
                     RRGTLREEQRWQPSDDRRRGVMPPALRTSPPQPTAPRFSGSQRERGAPRTLPYDRGHG
                     WNRQPVDRAHGVGRVRADAPQYRAAQPDRVARHARRDAGAVPVRPAVRAGMAGDRPRM
                     AR"
     gene            5560..6354
                     /locus_tag="AC16O9_09875"
     CDS             5560..6354
                     /locus_tag="AC16O9_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HIR6516964.1"
                     /translation="MRFLYGLPFALAWLAIVHAWRAEAIPALGWAFLAWVTLGAVAQI
                     AATAFLLAAMEARSFALGVAYSKTELLQIAVFGLAFLGDPLSMPVLLAVALGTLGVLL
                     LSPADPQRPFAALLTGLGTRPALLGLASGAGFAFAAIGYRGAALALEGSFLMSAATAL
                     AAAQVIQTALLGGWLLWRSPGVVRATLRLWRPSLFAGFMGAAASAGWFSAFALQPAAH
                     VRTLGLVELLFSYAVSRKLFSERMTRLEAAGMAFLAAGIAVITLTA"
     gene            complement(6338..7402)
                     /locus_tag="AC16O9_09880"
     CDS             complement(6338..7402)
                     /locus_tag="AC16O9_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HIR6516965.1"
                     /translation="MGAVFKYVLPVEETHWKVQGASETAFTWNYDTRSEELLRLYSKG
                     KQQQWDAEKRIDWSQDVDPDDPMQIAEEMMPLYGTPLYQRLSARQISDLRYHSQVHNL
                     SQFLHGEQGALVCSARIVQDAPTLESKFYAATQVMDEARHVEAYRRLLKEKFRFVYPI
                     SPPLKRLLEQALTDKRWDFTYLGMQVLIEGLALAAFQRIRDYAKNPLCQAVNAYVMQD
                     EARHVAFGRIALREYYPQLSAAERREREQFVIEGSYFLRDRLNSEEMWEAIGIDPKDA
                     IGAHAASEGQVRFRKRLFSRIVPTVKDIGLWSDAVQKAYADMGVLHYGERNAQEMLDH
                     DAAVAEEFDRLVAARARPSA"
     gene            complement(7402..8193)
                     /locus_tag="AC16O9_09885"
     CDS             complement(7402..8193)
                     /locus_tag="AC16O9_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6516966.1"
                     /translation="MGAADAGQDLLYEVRDGIARVTFNRPQARNALTFAMYDRLAEIC
                     AEADKDRSIKAMLLTGAGEKAFAAGTDISQFRAFDKPQDALDYEARIDRVLGALERCR
                     VPTVAAIRGACTGGGAGIAVCCDLRIAGTSLRTGFPVARTLGNCLSSGNYARLAALIG
                     PARVKDMIFRARLLEAPEALAIGLVTEVVDDAQVMARAEEVTQLVASQAPLTLQATKE
                     SLLRLRPSLAKGEGDDLILMCYMSQDFREGMQAFLEKRAPQWKGR"
     gene            8259..>9077
                     /locus_tag="AC16O9_09890"
     CDS             8259..>9077
                     /locus_tag="AC16O9_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6516967.1"
                     /translation="MRTTKCLGALAAAAALVLAVSPAQSQDSYPSKRLDWTIAFGPGG
                     GNDIMSRTLISILEKYKLYPETIVADNKPGGSGAKGWGFLFAQKGNPYHVSTTSGSFI
                     TTPLQANTPWQPGSFTPVALLASDDMLLLVSKDSKIKTLAEFIAAAKAKPPSIGGIGA
                     VNIDFIVPKLVSEKAGFTFNYVSFNKQGELITALLSNSLDAAMSNPGEVLGLLQSGDM
                     RALAFSGKSTPAALGTVPTFAELGYDIGISLPRGLVLPPDGPKLAQDWWIATMKK"
BASE COUNT         1358 a         3174 c         3091 g         1454 t
ORIGIN      
        1 gctcgcgtac tactacctcg gcaagttcga catcgtcgct ttcggtacag gcgcgatcct
       61 ggaccagctc gagacccggg cgctggcggt ctccggcgtc tactcggcgc cgctcgggca
      121 cggatacaac ttcatcgcca agctcggcat cgcgttcacg gaggcgcagt acgattgcag
      181 gacgggttgc ggcgtggccc cctttatcga caccaggcgc cgcggcaatt cggggcttat
      241 cggactgggc atcacctcgc aggtgtcgtc caacgtcgcg ttgcggatgg agttcgagca
      301 catcggcaac gttcggcatg ccatgtcgaa taccgtcttc ttcaacgacg gctacgacat
      361 gttctcggtg ggcctgcacc tgaatttctg atcgggtagg acgttccaac ggcccttagg
      421 ttgggccgtg tgccgagcaa gctcttgttt tgattgacgg aaacctgccg tccctgtata
      481 aaggtacggc aggattctca agcagagaaa aagctggtcc gccggcggcc cgagtgccgc
      541 gctcatcagt tgcttcgttt cctttccttg atcattccgc agggcaagcg ccctttcatt
      601 catcactggg gaagcaagac atgagcgcag gtaccgtgaa gtggttcaac gacgccaagg
      661 gtttcgggtt catcacgccg gaggacggca gcaaggatct gtttgtgcat ttttcggcga
      721 tccagggcgg cggcttcaag acgctgaagg agaacgacaa ggtgcagttc gacgtggtgc
      781 agggcccaaa gggcacccag gccaccaacg tcaggcccgc ctgatcgcga cgacggcaat
      841 ccaagcccgg tcgcaccggg cttttttgtt tccacgggga gcgggcatgg acaagcgcaa
      901 gcgatggcgg gagcaggagc agcaggtggt ggagcgctgg aacgccgccg aagcgcgcta
      961 ccgcgaggtg cacgcggcgc tgcagcgcct ggcgggcaac ggcgccgaag ccgaggccct
     1021 gaaacaggaa gtcgagcgcg cgcgcgccga catcgaagcc gtgcggcggc aggtggcgcg
     1081 cctcaaggtc gaattcacat cgggaaagag atactgaatg aacacgcgaa acaacctgac
     1141 gcgcggccgt gccgcgctgt ggagccggga caagatcgag ttgctcagca cgccggaggt
     1201 gcgcaacctg cacgccaacg ccgagcggct cctcgagacc gagatcgccg cgctgtgcct
     1261 cgaagtgctg ggaacccggc cgcgcagcgg cgtcgcggcg cctgccaagc ccgcgccgcg
     1321 cgtccggcgc gccaaggcca aggccgagca agccgcctag cgcgcgggcg cgctacactg
     1381 cggccgccat gtgcggccgc tacgtcagtc ccgagcaagc gtcgatcgag cgcgcctgga
     1441 agatcgaccg cgcgagcagc aacccgtttc cgcgccgctt caacgtgcag cccaccactg
     1501 ccgtgccgat gctgcgcctc gaggacggtg cgcgcacgct gagcggcgcg cgctggggcc
     1561 tgattccgca ctggtggaag caggacaagc cgcccagctt cagcatcaat gcgcggctcg
     1621 aggacatctc gcgcaagccg atgtggcgcg atcctattcg ccgctcgcgc tgcctggtgc
     1681 cggccgaagg ctggtacgag tggcaggaaa ccccgagcgg caagcagccg cactacatca
     1741 cgcgccgcga ccgccgcccg ttctgctttg cggggctgta cgcgcactgg cgcgatacgc
     1801 tgagctgcgc catcctcacc acgcaagcgc tcggaccgat cgcggaggtg cacgagcgca
     1861 tgccggtggt gctgcccgag gacgctttcg gcgcctggct ggagcccgcg atgcatgacg
     1921 cagcagtggc gaccacgatg gcgcaggcgc cgatggcgcc ggaggacttc gagcactacc
     1981 cggtcagcag gcgcgtgaac aacgctcgca acgagggccc ggacctgatc gagccgctgc
     2041 gcgcctagcg cttgcgggcg gcggcggctc tctccttgcg cgccgccatg atcttctcgt
     2101 agcactcggc gcacacttcg cgccgccgct cgccgccggg cagcacgcgg aactgcgcgc
     2161 gcggcttcga gtgctggccg tgcatgcaga acttgagcgg gctgagattg ggcatggtcc
     2221 ggggcgccgg ggccgcgccg ctataatcgt gcggccgact ccatcgacgc ttcgagcacc
     2281 gtatcacatg tggccgtttc accgaaactt ggccgtaccg ctgctggccg cgctcgtcgc
     2341 gggcgcgggc ggtgccgtgc aggcctttga cctccagggg caccgcggca cccgcggcac
     2401 cgccccggag aacacgctac ccgggtttgc tgcggcgctc gcgacgggcg tcgacacgct
     2461 ggagctggat acgggcgtga cgcgcgacgg ggtcgtcgtg atccatcacg accggcgcct
     2521 caaccccgac atcgcccgcg gccccgacgg ccgctggatc gaggctccgg ggccgctgct
     2581 gcttgagctc gatttcaagg acctgcagcg ctacgacgta gggcgcctgc gtccgggcag
     2641 cgactatgcc gcgcgctttc cggagcagac tcccgccgac ggcgcgcgca tcccccgcct
     2701 cgccgagctt ttcgctctgg tacgcaaggc cggcaacgac caggtgcgtt tcaacatcga
     2761 gaccaagatc tcgccggcgg cgccggccga aacgctgccg cccgaggcgt ttgcgcgcgc
     2821 gctgatcgcg gagatccgcg ccgccggcat ggccgggcgc acgaccgtgc agtccttcga
     2881 ctggcgcacg ctgcaggccg tcgagcgcga ggcgcccgag atcgccacgg cgtacctgac
     2941 cggccggcgc cgcggcggct cgcaaccgca ggcagtgcac gccgcgggcg gcaggatctg
     3001 gtcgccgaac tacgaagagc tcgattccgt ggcgctggtc gaagcgcgca ggctcgggct
     3061 gaaggtcgtg ccctggacca tgaacgagcc cggcttcatc gagcgctttc tcgaccttgg
     3121 cgtggacggc atcatcaccg actacccgga gcgtgtgcgc gctgaactcg tgcggcgcgg
     3181 catgccgctg cccgcgcgca ccccggtgga gccctgaggg tgccgccggt cacgggcgcg
     3241 ctgctcgccg ccaacgtcct cgttttcatg ctgcagcttg ccggcggcct gccgctcgtc
     3301 gagcacttcg cgctgtggcc gccggccacc tcgggaccgc gcgccggccc gcccttcgag
     3361 ctgtggcagc tgctgagcta cagctttctg cacggcagcc tgctgcacct gatgttcaac
     3421 atgctcgcgc tgtacacgtt cggccgcgac gtcgagcgcc tgttcggctc gcagtggtat
     3481 ctgcagttct acttcgccag cgtggtagcg gcggcgctgt cgcacctggt ggtcaccggc
     3541 tggatgggcg cgccgccgta tcccacggtc ggcgcttcgg gcggcgtcta cggcgtgctg
     3601 ctcgcctacg gcatctattt cccgcggcgc acggtgctgc tgttgtttcc gccgatcccg
     3661 atgccggcgc gcgtgttcgt ggtgctgttc gcggccctgg agttgtactt cggcgtcacc
     3721 ggcacggcct cgggcatcgc gcatttcgcg cacctgggcg gcatgctcgg aggctggctg
     3781 atgattcgca gccggaggcg ccgccgcggc tagttatggt gtataaggga ggtctatgct
     3841 cagagaactc cctggtcagc agtcccgcag ccgattcatg gtcggcaagt acgaagtgat
     3901 cgcccagccc atccccggat ccgcgcacat gctgcgctac acggttttcc tgaagggcac
     3961 gcgcatcggg gcgatggcga gcgtgcccac cgagtccgat tgccgcttcc tcgagaaccc
     4021 gccgccggta ccgccggtga agccgttcca ggtgttttac cgcccgggcc ggccgaagaa
     4081 gggcagccag cccgcgagcg ccgcggaccg cgccctcggc agcacgccgc acgaggacct
     4141 gcccgcaggc ctcgccatcc cgtcgctgga gaagtcggcc gagtagaaac atcctgttgc
     4201 aatttgcgcc agttttaggg cgattgcaga cagcctaagc ggctacaaag tgggcggagg
     4261 caacgcccat gagaaagctc gtcatcgccc tgctcgcagt tctcgccacc gcgtcgggcg
     4321 cgctggccga ggagcgcgcg ccctactcgc aagccgagct cgaccagatg ctcgcgccga
     4381 tcgcgctgta tcccgacgcg ctgctgtcgc aggtgctgat ggccgcgacc taccccctag
     4441 aggtggtgga agcggcccgc tggtcgcgcg cccactccga cctcaagggc gaagacgcag
     4501 tccgggccgt tcaggaccag gattgggatc cgagcgtgaa gtcgctgctc gccttccccg
     4561 atctcctgac gcgcatggac gaggagctcg attggacccg gcgcctcggc gatgccttcc
     4621 tcgagcagga gccgcatgtc atgcagaccg tgcagcggct gcggcgcaaa gcagaggcgg
     4681 cgagccagct cggctccggc gagcacctgc gggtgctcga ggaggacggc gtcatcgtca
     4741 tcgagcaggc gaatccgcgc gtcgtctatg tcccgtacta cgaccccctc gtcgtctacg
     4801 gcgcctggtg gtggcccgcc catccgccgg tggtctgggc gccgtggtcg ggatacaccg
     4861 tgtatcggcc gggcatctgg tggggcgtgg gcgtcggcat ttcctcgggc ttcttctacg
     4921 gcggggtgcg ttggatcgac cggcgcgtgg tcgtcgtgcg cccgagcccc tacgatgcgc
     4981 gtccgctcgc gaccggccca tggcggcaca atccggcgca ccgccgcggc atcgtgtacc
     5041 gccaccccga aacgcaacgg cgtttcgcgc ccgtggagcc gcgcctccag cgacccgcag
     5101 tgcgccagga atcgcagcgc gaggaacggc gcgcgccgcc gcgggcggag ccgcgctggc
     5161 cgccgcgcga ggaacggcgc gcgccgcaac gggacgagcc gcgctggcag ccgcgcgagg
     5221 aacggcgcgg gccgcaacgg gacgagcggc gctggcagcc gcgcgaggaa cggcgcggga
     5281 cactgcggga ggagcagcgc tggcagccga gcgacgatcg ccgccgcggc gtgatgccgc
     5341 cggcgctgcg caccagcccg ccacagccca ccgcaccgcg cttctcgggt tcgcaacgcg
     5401 agcgcggcgc gccgcgcacg ctgccctatg atcgcggcca tggatggaac cggcagcctg
     5461 tggatcgcgc tcacggtgtg ggccgcgttc gcgcagacgc tccgcaatac cgcgcagcgc
     5521 agcctgaccg cgtcgctcgg cacgctaggc gcgacgctgg tgcggttcct gtacggcctg
     5581 ccgttcgcgc tggcatggct ggcgatcgtc cacgcatggc gcgctgaggc catcccggcg
     5641 ctgggctggg cgttcctcgc ctgggtcacg ctcggtgcgg tggcgcagat cgccgccacc
     5701 gcgttcctgc tcgcggcgat ggaggcgcgc agcttcgcgc tcggcgtggc gtactccaag
     5761 accgagctgc tgcagatcgc ggtcttcggg ctcgccttcc tcggcgatcc gctctccatg
     5821 cccgtgctgc tcgccgtagc gctcggcacg ctcggcgtgc tgctgctctc gcccgccgat
     5881 ccgcaacggc cgttcgccgc gctgctgacc ggcctcggca cgcgacccgc gctgctcggc
     5941 cttgcctccg gcgccggttt cgccttcgcc gccatcggct accgcggcgc ggcgctcgcg
     6001 ctcgaaggct cgttcctcat gtcggcggca acggcgcttg cggcagcgca ggtgatccag
     6061 accgcgctcc tcggcggctg gctgctgtgg cggagccccg gcgtggtgcg cgcgacgctg
     6121 cgactgtggc ggccgtcgct gttcgcaggc ttcatgggcg ccgcggcgtc cgcgggctgg
     6181 ttcagcgcct tcgcgctgca gcccgcggcg cacgtgcgca cgctcggcct cgtcgagctg
     6241 ctgttcagct acgcggtgtc gagaaagctc ttcagcgagc gcatgacccg gctggaagcc
     6301 gccggcatgg cgttcctcgc cgccggcatt gccgtgatta cgctgacggc ctagcgcgcg
     6361 ccgcgaccag cctgtcgaac tcctcggcca ccgcggcgtc gtggtcgagc atctcctgcg
     6421 cgttgcgctc gccgtagtgc agtaccccca tgtcggcgta cgctttctgc accgcgtcgc
     6481 tccacaggcc gatgtccttc accgtcggca cgatgcggct gaacaggcgc ttgcggaacc
     6541 gcacctggcc ctcggaggcg gcgtgcgccc ctatcgcgtc cttggggtcg atgccgatcg
     6601 cttcccacat ctcttccgag ttcaggcggt cgcgcaggaa gtacgagcct tcgatcacga
     6661 actgctcgcg ctcgcgccgc tcggcggccg agagctgcgg gtagtactcg cgcagcgcga
     6721 tgcgtccgaa ggcgacgtgg cgcgcctcgt cctgcatgac gtaggcattc accgcctggc
     6781 acaggggatt cttcgcgtag tcgcggatcc tctggaacgc ggcgagcgcg agcccttcga
     6841 tcagtacctg catgccgagg taggtgaagt cccagcgctt gtcggtcagc gcctgctcca
     6901 gcaggcgctt gagcggcggc gagatcgggt agacgaagcg gaatttctcc ttcagcagcc
     6961 gccggtaggc ctcgacgtgg cgcgcctcgt ccatcacctg ggtggcggcg tagaacttcg
     7021 attccagcgt cggcgcgtcc tgcacgatgc gcgccgagca caccagggcc ccctgctcgc
     7081 cgtgcaggaa ctgcgacagg ttgtgcacct ggctgtggta gcgcaggtcc gatatctggc
     7141 gcgccgacag gcgctggtac agcggcgtgc cgtagagcgg catcatctcc tcggcgatct
     7201 gcatcggatc gtcggggtcc acatcctgcg accagtcgat gcgcttctcg gcgtcccact
     7261 gctgctgctt gcctttggag tacagccgca gcagctcttc gctccgcgtg tcgtagttcc
     7321 aggtaaaggc ggtctcggac gccccctgga ccttccagtg cgtttcctcg accggcagca
     7381 cgtacttgaa gacggcgccc attaccggcc tttccactgc ggcgcgcgct tctccaggaa
     7441 cgcctgcatg ccctcgcgga aatcctggct catgtagcac atcaggatca ggtcgtcgcc
     7501 ttcgcccttg gcgagcgacg ggcgcaggcg cagcagcgac tccttggtcg cctgcagcgt
     7561 gagcggcgcc tggctggcga ccagctgcgt cacctcctcg gcgcgcgcca tcacctgcgc
     7621 gtcatccacc acctcggtga ccaggccgat ggcaagcgct tccggcgcct cgagcagccg
     7681 cgcgcggaag atcatgtcct tcacgcgcgc cgggccgatc agcgcggcaa gccgcgcgta
     7741 gttgccgctg gacaggcagt tgccgagggt gcgcgccacg ggaaagcccg tgcgcaggct
     7801 ggtgccagcg atgcgcaggt cgcagcacac cgcgatgccg gcaccgcccc cggtgcaggc
     7861 gccgcggatc gcggctaccg tcggcacgcg gcagcgctcg agcgcgccga gcacccggtc
     7921 gatgcgcgcc tcgtagtcaa gggcgtcctg gggcttgtcg aaggcgcgga actgggagat
     7981 gtcggtgccg gcggcaaagg ccttctcccc ggcgccggtc agcagcatcg ccttgatcga
     8041 gcggtctttg tcggcctcgg cgcagatctc cgccaggcgg tcgtacatgg cgaaggtcaa
     8101 ggcattgcgc gcctgcgggc ggttgaaggt cacccgcgcg atgccgtcgc gcacctcgta
     8161 cagcagatcc tgcccagcgt ccgcagcgcc catgatgctc cccgtccttt tttccggaag
     8221 atgtgggaga ctagcgcagg aggaggagga gaaccaccat gagaacgacg aaatgcctcg
     8281 gcgcgctggc ggcggccgcc gcgctggtct tggccgtatc cccggcccaa tcgcaggaca
     8341 gctacccgtc caagcgcctg gactggacca tcgccttcgg gccgggaggc ggcaacgaca
     8401 tcatgtcgcg caccctgatc tcgatcctgg agaagtacaa gctctacccc gagaccatcg
     8461 tcgccgacaa caagcccggc ggcagcggcg ccaagggctg ggggttcctg ttcgcgcaga
     8521 agggcaaccc ctaccacgtc tccaccacca gcggcagctt cattaccacg ccgctgcagg
     8581 ccaacacgcc ctggcagccc gggagcttca cgccggtggc cctgctcgcc tccgacgaca
     8641 tgctgctgct ggtgagcaag gattcgaaga tcaagaccct ggcggagttc atcgccgcgg
     8701 ccaaggccaa gccgccgtcg atcggcggca tcggcgccgt caacatcgac ttcatcgtgc
     8761 caaagctggt gtcggagaag gccggcttca ccttcaacta cgtctcgttc aacaagcagg
     8821 gcgagctgat cacggcgctg ctgtccaatt cgctcgacgc ggcgatgtcg aacccgggcg
     8881 aggtactcgg cctgctgcaa tcgggcgaca tgcgggcgct cgccttcagc ggcaagagca
     8941 cgccggcggc gctcggcacg gtgcccacct tcgccgagct gggctacgac atcggcatct
     9001 cgctgccgcg cgggctggtc ctgccgcccg acgggcccaa gctagcccag gactggtgga
     9061 tcgcgaccat gaagaag
//
LOCUS       DBNHKE010000147        11848 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1136359, whole genome shotgun sequence.
ACCESSION   DBNHKE010000147 DBNHKE010000000
VERSION     DBNHKE010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1136359"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /gene="gspD"
                     /locus_tag="AC16O9_09895"
     CDS             <1..1057
                     /gene="gspD"
                     /locus_tag="AC16O9_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HIR6516968.1"
                     /translation="GGMVQADAASNALLISAPDALFAHIRSIIDRLDVRRAQIYVEAL
                     IAEIQADKAAEFGIQWQSVGDLTAPRSRVIGGTNFSGTGTGANILDAAINLGTLGPGL
                     NIGVVKGQVTIPGIGTVTNLSALARALETEAKANILSTPNLLTLDNEEARIVIGQNVP
                     FITGQYAQTGSATTATPFQTIERRDVGLTLRVKPQISEGGTVRLQIYQEVSSVRDSTN
                     EAGVITDKRSIESMVLVDDGQIIALGGLVQDSVSNGIEKVPLLGDIPLLGLLFRYETR
                     KQTKTNLMVFLRPVVLRDGAAFRGTTVERYREMRFDQEESQQPAHPVLPSYPAPEMPP
                     LEDEEEWPPDRPLMQWR"
     gene            1054..2547
                     /gene="gspE"
                     /locus_tag="AC16O9_09900"
     CDS             1054..2547
                     /gene="gspE"
                     /locus_tag="AC16O9_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009972599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR6516969.1"
                     /translation="MNAAADATLRLIPYSFAKAHGALLLEVTGDEARVCLREGSAPRT
                     LAELRRVLGLPVRVAAIEPAESFERRLADAYAQASQSAAYVADNLGQDMDLSRLVREM
                     PKVEDLLESEDDAPIIRMINVLLTQALRQGASDIHIEPYDKRSVVRLRVDGTLQTVAE
                     PHRALHAAILSRIKVMANLDIAEKRLPQDGRISLRLAGRQVDVRVATLPTGFGERAVL
                     RLLDKEGGRLELQGLGMDPTTLVAVDALVHQPHGIVLVTGPTGSGKTTTLYAALSRID
                     SLGLNIMTVEDPIEYELEGVAQTHVNSRIGMTFARALRSILRHDPDVIMIGEIRDLET
                     AQIAVQASLTGHLVLATLHTNDAVSAVTRLNDMGVESYLLASSLRGVLAQRLVRKLCP
                     ACKVAREPDEAECGELGVRRGEQTVYGPAGCAACHGTGYVGRTGIYELLNIDDEARRL
                     IHTAGAEHDLRAHAVAVGMRALRQDGLRWVRAGITSLEEVIRVTREA"
     gene            2586..3809
                     /gene="gspF"
                     /locus_tag="AC16O9_09905"
     CDS             2586..3809
                     /gene="gspF"
                     /locus_tag="AC16O9_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR6516970.1"
                     /translation="MPAYSYVAVDAQGRTRRGIVEAEVPRLARAGLRSEGLIPVSVTP
                     VDPEVRARGAGSWFARRQRLSATELALTTRRFALLLEAGLTIEQCLDALIEQAQDESS
                     GRMLAAVRAEVRSGQSLAAALERLPSSFPEIYRALIATGEHSGDLARVMSKLADYLEN
                     RLTMTQSAGLALLYPGIVAVVALVIVVGLLTYVVPQVVDVFERSRQALPFLTRAMLWT
                     SEALRGNLILIGGVLAGLAVAARLAWQSEAWRARWQARLLRMPVIGPLLQGISTARLA
                     STLAILAGSGVPLLQALAAGARVIGNLPLRTAVEEAAQLVREGSSLHRALDSRKLFPP
                     IFIHLVASGETSGRLADMLQQAATHQEVENARRLRVLTGILEPLIIVLMGVVVMLIVL
                     AILMPIIEMNQLIRP"
     gene            complement(3856..4242)
                     /locus_tag="AC16O9_09910"
     CDS             complement(3856..4242)
                     /locus_tag="AC16O9_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6516971.1"
                     /translation="MKHHVLILDDEADVRGAIRLQLKGTRFDVLEAENVDQAIALIGD
                     NPISIDVIICDVRMPGKSGVEAVEYFQRQYPNTPVIVLTGFPDVALAVDFMKKKDVVD
                     FLTKPVDKEKLVATVERAARGRKLFE"
     gene            complement(4239..5432)
                     /locus_tag="AC16O9_09915"
     CDS             complement(4239..5432)
                     /locus_tag="AC16O9_09915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6516972.1"
                     /translation="MTRSPDTRTELYELALHAAPSGIAVVDERGGIRFANRTLAEMFG
                     YGIEELLGQTVEILLPLEHAEAHRRHVQHYTQQPQQRPMGTGRDLEGRHRDGRRFPVE
                     VGLRPAQMATGRAVVATVIDITARKAIEERLRMHETQLEALVAERTRELRESQQEKDR
                     VVEQLIQAEKMAAVGTLVSGIGHEINNPLYAILATTEALAVEPRLAQCHAYALDILRH
                     VKSIAETVKNLSHYAQPGSRHDLQRVDVNAAVEGAVRLARRALHADAVEIRTDTDAVP
                     QILAKAEELQQVFFNVVRNAVQAISGRGRVDIRTASHERWVSVTVQDDGPGIAAENLK
                     RVFDPFFTTKGPDQGEGLGLYIVRQIVTRYRGTIEVENAAGRGARFVMRFPAAGANTT
                     AGENA"
     gene            5587..5727
                     /locus_tag="AC16O9_09920"
     CDS             5587..5727
                     /locus_tag="AC16O9_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516973.1"
                     /translation="MTGQALRTQPRAARRRLGGFDYFVLAGATVNALVIACLVGFWLI
                     AG"
     gene            5767..7899
                     /locus_tag="AC16O9_09925"
     CDS             5767..7899
                     /locus_tag="AC16O9_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6516974.1"
                     /translation="MSTEPVAGRIEPLPRYREIVLAVDSSDHANRGVEDGIALARRWG
                     ASVTATHVYAARLHDLRFRQMEGGLPERFRAEQELERQRDVHNDLITRGLSIISESYL
                     DQVERAGRAAGVRVARRALEGKNYRELSREANSGRYDLLVLGALGLGAVPESCLGTVC
                     ERVVRRSAIDTLVIKSPGRAVSAGPIAVAIDGSPRAYGGLMTALALAKEWQVPLHVIS
                     AFDPYYHYVAFNRIAGVLSEEAGRVFRFREQERLHEEIIDSGLAKIYEGHLQVARGIA
                     ADCEVPVQTRLLDGKPHDAIGRYLRELQPSLLVIGRLGIHADDALDIGGTAENLLRTA
                     PCAVLLSARQFTPQVERLAEVGVSWTSEAEERLRRAPSFVQSMARMAILRYAQERGHT
                     VITARLVEEATASLMPGRAAQAMAEIVAAHDAGELQRDQARDAPEWSDAARGRLAAVG
                     DASLRENVRLRSEKKARSVHAAQVAPEHVDAVVPAAGALHWEAAALARLSRVPEGFMR
                     EAARGRIESEARRSGAAAVTLDLAEAGLAAARAAMTELAGHEAAGASAPAPTASKCPF
                     AAMTRAAPPPEASAPAAQWTPEATARLQAVPEGFCRTMTERAVNTLARQNGLAQIDAG
                     FVQRILEIFETGARAASPTMPWADAARERLERAPEAVRGMLVREIEGWARRSGLDTVD
                     GRVVEAVKGEWQASGAFHLDPGDPRHVA"
     gene            7889..9163
                     /locus_tag="AC16O9_09930"
     CDS             7889..9163
                     /locus_tag="AC16O9_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6516975.1"
                     /translation="MSPEQPATPALSLLAINLTRRCNLACAHCYLDATTLAGGDADEL
                     ATHEVQALLEDVASLAQGTMVVLTGGEPLLRGDLDALIRHGTARGLPMVVGTNGTLLD
                     EARVGALKAAGALGLGISLDSLDAASHDRFRGRDGAWARTMAGIECCRRLGMDFQLHF
                     SVTDANAEELPAMAEFARSCGARSLNVFFLVCVGRGQTVSALAPHRYEALLAQLIELQ
                     SAYPGLIVRPRCAPHVKRVAHQLRPLQALNRISGREGDGCIAGLHYARVNHRGGVTAC
                     PYIEQEVGSLRTQRLSALWSGAEQFARLRAPALRGKCGACEYRRLCGGCRARPLARGG
                     GLMDADPLCGYRPRGGDVIEPLAVAQAAPTWAADAEQRLERVPVFLRALVRKRAEAYV
                     GGLGESEVNTRHLSEMVAARFGAGAAPGRGPA"
     gene            9160..10599
                     /gene="hemG"
                     /locus_tag="AC16O9_09935"
     CDS             9160..10599
                     /gene="hemG"
                     /locus_tag="AC16O9_09935"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR6516976.1"
                     /translation="MSRRDVIVVGGGISGLATAHCLASEGLDVELWEGTARVGGKIRT
                     AKRDGYRLESAATMVVNFRGALDGFMRGAGLEAAKRVRGPMSARYVLDADRLVEAPAG
                     MGELLRTRLLSRGAKLRMLAEPFARRGRDRHESVAAFVTRRLGREVLDKLFEPYVAGP
                     LASDAALAEARTTMPKLVALEKRYGSLALGALCARLAGRGGAALPEVFSFAGGMATLV
                     EHLARDGGFGVRRGVPVREAWPVHGGWRVRGGHAGAPQDAICRHLVISAPPQAAAEIL
                     RGVDADLARLLASVRFAPIRVVHTGFERSRIGHGLNGSGFLVPRGSAFAANGCLWLSR
                     LFPRCAPQERVLLTSYLGGSRNPDAAFQDPSRSLGEVMHMLRTLLDARGEPETLHVET
                     HERGLPLYHGGYSAQLAAMDERLGRLPGLHLEANYRGGVSVRDRIACAQKAAARIARQ
                     LEAARRPGGANSQPRESAVPIAPVAAAVP"
     gene            10596..10958
                     /locus_tag="AC16O9_09940"
     CDS             10596..10958
                     /locus_tag="AC16O9_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516977.1"
                     /translation="MSGARLDQAGLADSPRRGLWALGLFAGVLALLLLVSASADWYSR
                     EVSLPRYCDEPEDALARLETLRSGAPQSLDESRRDYMVAAKLEFILPRASGEPEALYR
                     LRVLQALRKQCHQKGLLG"
     gene            11077..>11848
                     /locus_tag="AC16O9_09945"
     CDS             11077..>11848
                     /locus_tag="AC16O9_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516978.1"
                     /translation="MRFASSLRGLTATGFVLLALSGCGADNGVNPLPASGSTAGAGAG
                     GTTTPPAGGGTGGTGGSGGTTTPPPDATSIVRSWYGIQANILQPFCVTCHQGAAQFAA
                     GLSWEVGQYEAIVTNGRLSTEIATMLEVNPNNPDASYLMWKLRGQGPAGEPIVGVRMP
                     ATGIALDPALITAIQEWIAAGAPLGDPADATSGGSTGGGGSGGTTNPPPDPTAIVPTW
                     YGVQANILQPFCVTCHQGAAQFAAGLSWEVGQYEAIVTN"
BASE COUNT         1675 a         4093 c         4344 g         1736 t
ORIGIN      
        1 cggcgggatg gtgcaggccg acgccgcctc gaatgcgctg ctcatctccg cgcccgatgc
       61 gcttttcgcg cacatccgct cgatcatcga ccggctcgac gtgcgccgcg cgcagatcta
      121 cgtcgaggcg ctgatcgcgg aaatccaggc cgacaaggcc gccgagttcg gcatccagtg
      181 gcagtcggtc ggcgacctga ccgcgccgcg cagccgggtc atcggcggca ccaatttcag
      241 cggcaccgga acgggcgcca acatcctcga tgcggcgatc aacctcggca cgctcgggcc
      301 ggggctcaac atcggcgtgg tgaaggggca ggtgacgatc ccgggcatcg gcacggtcac
      361 caacctgtcg gccctggcgc gcgcgctcga gaccgaagcc aaggcgaaca tcctgtcgac
      421 gccgaacctg ctgacgctcg acaacgagga agcgcgcatc gtcatcggcc agaacgtgcc
      481 gttcatcacc ggccagtacg cgcagaccgg cagcgcgacc accgcgacgc cgtttcagac
      541 gatcgagcgc cgcgacgtcg gcctgacgct gcgcgtcaag ccgcagatct cggagggcgg
      601 cacggtcagg ctgcagatct accaggaggt gtccagcgtg cgcgactcca ccaacgaggc
      661 gggcgtgatc acggacaagc gctcgatcga gtcgatggtg ctggtcgacg acggccagat
      721 catcgcgctc ggcggcctgg tgcaagacag cgtgagcaac ggcatcgaga aggtgcccct
      781 gctgggcgac atcccgctgc tcgggctgct gttccgctac gaaacgcgca agcagaccaa
      841 gaccaacctg atggtgttcc tgcgcccggt cgtgctgcgc gacggcgcgg cgttccgcgg
      901 cacgacggtc gagcgctacc gggagatgcg cttcgaccag gaagagtcgc agcagccggc
      961 gcacccggtg ctgccgtcct atcccgcgcc cgagatgccg ccgctcgaag acgaggagga
     1021 atggccgccc gaccgtcccc tgatgcaatg gcgatgaacg cggcggcgga cgcgacgctc
     1081 cgcctgattc cctactcgtt cgcgaaggcg cacggcgcgc tgctgctgga ggtgaccggg
     1141 gacgaggcgc gcgtgtgcct gcgcgagggc agcgcgccgc gcaccctggc cgagctgcgc
     1201 cgcgtgctgg gcttgccggt gcgcgtcgcg gccatcgagc cggcggaaag cttcgagcgg
     1261 cgactcgccg atgcgtacgc gcaggcgagc cagtcggcgg cctacgtcgc cgacaacctg
     1321 ggccaggaca tggacctgtc gcgcctggtg cgcgagatgc ccaaggtcga ggacctgctc
     1381 gaatccgagg acgacgcgcc gatcatccgc atgatcaacg tgctgctcac gcaggcgctg
     1441 cgccagggcg cctccgacat ccacatcgag ccctacgaca agcggtccgt ggtgcgcctg
     1501 cgcgtggacg gcacgctgca gaccgtggcc gagccgcacc gcgcgctgca cgcggcgatc
     1561 ctgtcgcgca tcaaggtgat ggcgaacctg gacatcgccg agaagcgcct gccgcaggac
     1621 gggcgcattt cgctgcggct cgcggggcgg caggtcgacg tgcgcgtggc gacgctgccc
     1681 acgggcttcg gcgagcgggc ggtgctgcgc ctgctcgaca aggaaggcgg ccgcctggag
     1741 ctgcaggggc tgggcatgga cccgacgacg ctggttgccg tggatgcgct cgtgcaccag
     1801 ccgcacggca tcgtgctcgt gacggggccg accggctcgg gcaagacgac gacgctctac
     1861 gcggcgctgt cgcgcatcga ctcgctcggg ctgaacatca tgacggtcga ggatccgatc
     1921 gagtacgagc tcgagggcgt tgcgcagacg cacgtcaatt cccggatcgg catgacgttc
     1981 gcgcgcgcgc tgcgctcgat cctgcggcac gaccccgacg tgatcatgat cggcgagatc
     2041 cgcgacctcg aaacggcgca gatcgccgtg caggcgagcc tgaccgggca cctggtgctc
     2101 gcgacgctgc acaccaacga cgccgtgagc gcggtcacgc gcttgaacga catgggcgtc
     2161 gagtcctacc tgctcgcctc gagcctgcgc ggggtcctcg cgcagcgcct ggtgcgcaag
     2221 ctctgcccgg cctgcaaggt cgcgcgcgag ccggacgagg ccgagtgcgg ggagctcggc
     2281 gtgcgccgcg gcgagcagac ggtgtacggc cccgcggggt gcgcggcctg ccacggcacc
     2341 ggctacgtcg ggcgcaccgg catttacgag ctgctcaaca tcgacgacga ggcgcgccgc
     2401 ctcatccata cggcgggcgc cgagcacgat ctgcgcgccc atgcggtcgc ggtcggcatg
     2461 cgcgcgctgc gtcaggacgg actgcgctgg gtacgcgccg gcatcacctc tctcgaggaa
     2521 gtgatccggg tgacccgtga ggcctgagcg ccggcgcggc gtggcgggcg ggacaccgcc
     2581 gggacatgcc cgcgtatagc tacgtcgccg tcgacgccca gggccgcacg cggcgcggga
     2641 tcgtcgaggc cgaggtgccg cggctggcgc gtgccgggct gaggagcgaa gggctcatcc
     2701 cggtttcggt cacgccggtc gacccggagg tgcgcgcgcg cggcgccggc agctggttcg
     2761 cgcgccgcca gcgcctctcg gcgacggagc tggcgctgac cacccggcgc ttcgcgctgc
     2821 tgctcgaggc gggcctgacc atcgagcagt gcctggacgc gctgatcgag caggcgcagg
     2881 acgagtcgag cggccgcatg ctcgcggcgg tgcgggccga agtgcgctcg gggcagagcc
     2941 tcgccgcggc gctggagcgc ctgccgtcgt cgttcccgga aatctaccgc gcgctgatcg
     3001 ccaccggcga gcactccggc gacctggcgc gcgtcatgtc gaagctcgcg gactacctcg
     3061 agaaccgcct gacgatgacg cagagcgcgg ggctcgcgct gctctatccg ggcatcgtcg
     3121 cggtcgtcgc gctggtcatc gtcgtggggc tgctgaccta cgtcgtgccg caggtcgtcg
     3181 atgtcttcga gcgcagccgc caggcgctgc ctttcctgac gcgcgccatg ctctggacga
     3241 gcgaggcgct gcgcggcaac ctcatcctga tcggcggcgt gctcgccggg ctggcggtgg
     3301 ccgcgcgcct cgcctggcag agcgaggcct ggcgcgcgcg ctggcaggcg aggctgctgc
     3361 gcatgccggt catcggaccg ctcctgcagg ggatctcgac cgcgcggctc gccagcacgc
     3421 tggcgatcct ggcgggcagc ggcgtgcccc tgctgcaggc gctcgcggcc ggggcccggg
     3481 tcatcggcaa cctgccgctg cgcaccgccg tcgaggaggc ggcgcaactc gtgcgcgagg
     3541 gcagcagcct gcaccgcgcg ctcgacagcc gcaagctgtt cccgccgatc ttcattcacc
     3601 tggtggcctc cggcgagacg agcggacggc tcgcggacat gctgcagcag gcggccaccc
     3661 atcaggaagt ggagaacgcc cgccgcctgc gcgtgctgac cggaatcctc gagcccctga
     3721 tcatcgtgct catgggggtc gtcgtcatgc tgatcgtgct cgcgatcctc atgccgatca
     3781 tcgagatgaa ccagctgatc cgcccctgag cgcaaggcgc gcgcggcggc cggcgcggtg
     3841 cgcccccccg cgcgactact cgaagagctt gcggccccgc gcggcgcgct ccacggtggc
     3901 aacgagcttt tccttgtcca ccggcttggt caggaaatcg accacgtcct ttttcttcat
     3961 gaagtccacg gcgagggcga cgtccggaaa gccggtgagc acgatgaccg gcgtattcgg
     4021 atactggcgc tggaagtact cgacggcctc gacgccgctc ttgccgggca tgcgcacgtc
     4081 gcagatgatg acgtcgatgc tgatcggatt gtcgccgatc agcgcgatcg cctgatcgac
     4141 gttctccgcc tcgagaacgt cgaaccgcgt gcccttgagc tgcagccgga tggcgccgcg
     4201 cacatcggct tcgtcgtcca gaatcagaac gtggtgtttc atgcattttc ccctgcggtg
     4261 gtgtttgcgc cggcggccgg gaatcgcatc acgaatcggg cgccgcggcc ggcggcattc
     4321 tcgacttcga tggtgccccg gtagcgggtc acgatctggc ggacgatgta gagcccgagg
     4381 ccctcgccct ggtcgggtcc cttggtggtg aagaacgggt cgaagacccg cttcaggttc
     4441 tccgcggcga tgcccggccc gtcgtcctgc acggtgacgc tgacccagcg ctcgtgcgac
     4501 gccgtgcgga tgtcgacccg gccgcgcccg gatatcgcct ggacggcgtt gcgcacgacg
     4561 ttgaagaaca cctgctggag ctcctcggcc ttggccagga tctgcgggac ggcgtcggtg
     4621 tcggtgcgga tctcgacggc gtcggcgtgc agcgcgcggc gcgcgaggcg cactgccccc
     4681 tcgaccgcgg cgttgacgtc cacgcgctgc aggtcgtggc gcgagccggg ctgcgcatag
     4741 tgcgacaggt tcttcacggt ctcggcgatg ctcttcacgt ggcgcaggat gtccagggcg
     4801 taggcatggc actgcgcgag gcgcggctcg accgcgagcg cctcggtcgt ggcgagaatc
     4861 gcatagagcg ggttgttgat ctcgtgaccg atgccgctca ccagcgtgcc cacggcggcc
     4921 atcttctcgg cctggatgag ctgctccacg acgcggtcct tttcctgctg cgactcgcgc
     4981 agctcgcgcg tgcgctcggc cacgagcgcc tcaagctgcg tctcgtgcat gcgcagtcgc
     5041 tcctcgatgg ccttgcgcgc cgtgatgtcg atgaccgtgg ccaccacggc gcggcccgtc
     5101 gccatctgcg cggggcgcag cccgacttcc acggggaacc ggcgtccatc gcggtgccgg
     5161 ccctcgaggt cgcggccggt gcccatgggc cgctgctgcg gctgctgcgt gtagtgctgc
     5221 acatggcggc ggtgcgcctc ggcatgctcc aagggcagca ggatctcgac cgtctggccg
     5281 aggagctcct cgatgccgta accgaacatc tcggcgagcg tgcggttggc gaagcgaatg
     5341 ccgccgcgct cgtcgaccac ggcgatgccc gagggcgccg cgtgcagcgc cagctcgtac
     5401 agctcggtcc gagtatcggg gctgcgtgtc attcgcggat gcgcgtgctg cgcggctggc
     5461 attgtgtgca gcgaccgggc ggctgcgcaa gcgcggcgcg cggaccccgg cgtgtcgcgt
     5521 gtcacccatg tgtcactggc gcctgataag agcacgtcgc atgaacagcg cttcccgccg
     5581 acgcgcatga ccggtcaggc cctgcggacg cagccccgcg cggcgcgccg gcggctcggc
     5641 ggcttcgatt acttcgtcct ggcgggggcg acggtgaatg cgctggtgat tgcctgcctg
     5701 gtcgggtttt ggctcatcgc cggctaggcg cgccggcacg catcgcaagg aggagtggag
     5761 gtgaatgtga gcaccgaacc cgtggctggg cgcatcgagc cgttgccgcg ctaccgggaa
     5821 atcgttctcg ccgtggattc ctcggatcac gccaatcgcg gcgtggagga cggcatcgcc
     5881 ctcgcccggc gctggggcgc cagcgtcacc gcaacgcacg tctacgcggc ccggctgcac
     5941 gacctgcgct ttcgccagat ggagggcgga ctgcccgagc ggttccgcgc cgagcaggag
     6001 ctcgagcggc agcgggacgt gcacaacgat ctgatcacgc gcggcctgtc catcatttcc
     6061 gagtcctacc tggaccaggt ggagcgcgcc gggcgcgcgg ccggcgtgcg cgtcgcgcgc
     6121 cgggcgctgg agggcaagaa ctaccgcgag ctctcccggg aggccaacag tggccgctac
     6181 gacctgctgg tgctcggcgc cctgggcctg ggggcggttc ccgaatcctg cctcgggacc
     6241 gtgtgcgagc gcgtggtgcg ccgctccgcg atcgacacgc tggtgatcaa gtcgccgggc
     6301 cgcgccgtct cggcgggccc gatcgccgtg gcgatcgacg gctcgccgcg cgcctatggc
     6361 ggcctgatga ccgccttggc gctcgccaag gagtggcagg tgccgctgca cgtgatctcg
     6421 gcgttcgatc cgtactacca ctacgtcgcg ttcaaccgca tcgccggcgt gctctcggag
     6481 gaggccgggc gcgtgttccg tttccgggag caggagcggc tgcacgagga gatcatcgat
     6541 tcgggcctcg ccaagatcta cgagggccac ctgcaggtcg cgcgcggcat cgccgccgac
     6601 tgcgaagttc cggtgcaaac ccggctgctc gacggcaagc cccacgacgc catcgggcgc
     6661 tacctgcgcg agctgcagcc ctcgctcctg gtgatcggca ggctcggcat ccatgccgac
     6721 gacgcgctcg acatcggcgg cacggcggag aacctgctgc gcacggcgcc ctgcgcggtg
     6781 ctgctcagcg cgcgccagtt caccccccag gtcgagcggc tggccgaggt gggcgtgtcc
     6841 tggacgagcg aagccgaaga gcgcctgcgg cgcgcgccca gcttcgtgca gtcgatggcg
     6901 cgcatggcga tcctgcgcta cgcgcaggag cgcggccaca ccgtgatcac cgcccggctc
     6961 gtcgaggaag ccacggcgtc gctcatgcct gggcgcgcgg cacaggcgat ggccgaaatc
     7021 gtcgccgcac acgacgcggg cgagctgcag cgggaccaag cacgcgatgc gcccgagtgg
     7081 tccgacgccg cgcgcggccg gctggcggcg gtcggcgacg cctcgctccg ggaaaacgtg
     7141 cgcctgcgct cggagaagaa ggcgcgcagc gtgcacgcgg cgcaggtcgc gccggagcac
     7201 gtggatgccg ttgtgccggc ggccggcgct ctgcactggg aagctgccgc cctggcgcgc
     7261 ctttcgcgcg tgccggaagg attcatgcgg gaagccgcgc gcgggcgcat cgaaagcgag
     7321 gcgcggcgct cgggcgccgc cgccgtgacg ctcgatctgg ccgaggcggg actcgcggcc
     7381 gcacgcgcgg cgatgacgga gctggcgggg cacgaggcgg ccggcgccag cgcgccagcg
     7441 ccgacggcaa gcaagtgtcc tttcgccgcc atgacgcggg cggcgccgcc gcccgaagcg
     7501 agcgccccgg ccgcgcaatg gacgcccgag gccaccgccc gcctgcaggc ggtgcccgag
     7561 gggttttgcc gcacgatgac cgagcgcgcg gtcaacacgc ttgcccggca gaacggtctg
     7621 gcgcagatcg acgcgggttt cgtgcagcgg atcctggaga tcttcgagac gggcgcgcgc
     7681 gctgcctccc ccacgatgcc ctgggccgac gcggcgcgcg agcgcctcga gcgggcgccc
     7741 gaagcggttc gcggcatgct ggtgagggaa atcgaaggct gggcgcggcg cagcggcctg
     7801 gacacggtgg acggccgcgt ggtcgaggcc gtgaagggcg agtggcaggc gagcggcgcg
     7861 tttcacctgg acccggggga tccgcgccat gtcgcctgag caaccggcga cgccggcgct
     7921 ttccctgctc gcgatcaacc tcacgcggcg ctgcaatctc gcgtgcgcgc actgctatct
     7981 cgacgcgacc acgctcgccg ggggcgacgc ggacgagctc gccacgcacg aggtgcaggc
     8041 gctgctcgag gacgtggcga gcctcgcgca gggcacgatg gtggtgctga cgggcggcga
     8101 gccgctgctg cgcggggacc tcgatgcgct gatccgacac ggcaccgcgc ggggtctgcc
     8161 gatggtggtc ggcaccaacg gcacgctgct tgacgaggcc cgcgtgggcg cgctgaaggc
     8221 cgcgggcgcg ctcgggctgg gcatcagcct ggattcgctc gacgccgcga gccacgaccg
     8281 cttccgaggt cgggacgggg cctgggcgcg gaccatggcg ggcatcgaat gctgccgccg
     8341 cctcggcatg gacttccagc tgcacttctc ggtgaccgac gcgaacgccg aggagctgcc
     8401 ggcgatggcc gagttcgcgc gcagctgcgg tgcgcgctcg ctcaacgtgt tcttcctggt
     8461 gtgcgtgggc cgcggccaga cggtgagcgc gcttgcgccg caccgctacg aggcgctgct
     8521 cgcgcagctc atcgagctgc agtcggccta ccccggcctc atcgttcggc cgcgttgcgc
     8581 gccgcacgtg aagcgcgtgg cgcaccagct gcggccgctg caggccctca accgtatcag
     8641 cgggcgtgag ggcgacggtt gcatcgcggg cctgcactac gcgcgcgtca accatcgcgg
     8701 cggcgtcacg gcctgcccgt acatcgagca agaggtcggc agcctgcgca cgcaaaggct
     8761 gtcggcgctt tggtcgggag cggagcagtt cgcgcggttg cgcgcgcccg cgctgcgggg
     8821 caagtgcggc gcctgcgaat accgcaggct gtgcggcggc tgccgggctc gcccgctcgc
     8881 gcgtgggggc gggctcatgg acgcggaccc tctatgcgga taccggccgc ggggcggcga
     8941 tgtcatcgag ccgcttgccg tcgctcaagc cgcccccacg tgggccgcgg atgccgagca
     9001 acggctggag cgcgtgcccg tgttcctgcg cgcactggtc aggaagcgcg ccgaggccta
     9061 cgtcggcggt ctcggcgaat ccgaggtgaa cacgcggcac ctctcggaga tggtcgccgc
     9121 gcgattcggt gcgggcgccg cgcccgggcg cgggccggca tgagcaggcg cgacgtcatc
     9181 gtcgtcgggg gcgggatctc cggcctcgcc acggcgcatt gcctggcgag cgagggactc
     9241 gacgtggagc tgtgggaagg cacggcgcgc gtgggcggaa agatccgcac cgcgaagcgc
     9301 gacggctatc ggctggagag cgccgcaacc atggtggtga acttccgcgg cgcgctggat
     9361 ggattcatgc gcggcgccgg gctcgaagcc gccaagcgcg tgcgcgggcc gatgtcggcg
     9421 cgctacgtcc tcgatgccga ccggctggtc gaggcgcccg ccggcatggg cgagctgctg
     9481 cgcacgcgcc tgctgagccg cggggccaag ctgaggatgc tggcggagcc gttcgcccgg
     9541 cgcggccgcg accggcacga atccgtggcg gccttcgtca cccgccgtct cggtcgcgag
     9601 gttctcgaca agctgttcga gccctacgtc gcggggcccc ttgcgagcga cgccgcgctc
     9661 gcggaagcgc ggaccacgat gcccaagctg gtcgcgctcg aaaagcgcta tggcagcctc
     9721 gcgctgggcg cgctctgcgc ccggctggcc gggcgcggcg gcgcggcact gcccgaggtg
     9781 ttctcgttcg cgggcggcat ggcgaccctc gtcgagcacc tcgcccgtga cggcggattc
     9841 ggcgttcggc gcggcgtgcc ggtgcgcgaa gcctggccgg ttcacggcgg ctggcgcgtg
     9901 cgcggcgggc acgcaggggc gccgcaagac gcgatctgca ggcacctcgt gatcagcgcg
     9961 ccgccgcagg ccgccgcgga aatcctgcgc ggcgtggatg cggacctcgc ccgattgctc
    10021 gcgtcggtcc gctttgcgcc catcagggtc gtgcacacgg gattcgagcg ttcgcgcatc
    10081 ggccatgggc tgaacggcag cgggtttctg gtgccacggg gcagcgcatt cgccgccaac
    10141 gggtgcctct ggttgtcgcg gctgttccca aggtgcgcgc cgcaggagcg cgtcttgctg
    10201 acgagctacc tgggcggctc gcgcaatccg gatgccgcat tccaggaccc ctcgcgcagc
    10261 ctgggcgagg tcatgcacat gctgcgcacg ctgctagatg cgcgaggcga gcccgaaacg
    10321 ctgcatgtcg aaacccacga gcgcgggctg cccctttatc acggcggcta ttccgcgcag
    10381 ctcgccgcca tggacgagcg gctcggccgc cttcccgggc tgcacctgga agcgaattac
    10441 cgcggcggcg tctcggtgcg cgatcggatc gcgtgcgcgc agaaggcggc cgcgcgcatc
    10501 gcccgccagc tcgaggcagc gcgtcggccc ggtggcgcga attcgcagcc gcgcgaatct
    10561 gccgtgccca tcgcgccagt cgcggcagcc gttccatgag cggggcgcgg ctcgatcagg
    10621 ccgggctcgc cgactccccc cggcgcgggc tttgggcgct ggggctgttc gccggggtgc
    10681 tggcgctgct cctgctcgtc tcggcgagtg ccgactggta ttcgcgcgag gtgtcgctgc
    10741 cgcgatattg cgatgagcca gaggacgcgc tggcgcgact ggagacgctg cgttcgggcg
    10801 cgccccagtc gctcgacgaa tccaggcgcg actacatggt cgcggcgaag ctcgaattca
    10861 tcctgccgcg cgcatctggc gagccagagg cgctgtatcg cctgagagtg ctccaggcgt
    10921 tgcgaaagca atgccaccag aagggtcttt tgggctaaaa ttccctattt attaacaaaa
    10981 gcttgatgtg aatcaaaaat cttggtgggc aagggtgaat acagttcctt tactcggtgt
    11041 tttgctcatg ggcgggagag gagcggagga ggtcagatga gatttgcgtc cagcttgcgc
    11101 gggctgacgg ccacgggatt cgtcctgctc gcgctgagcg gttgcggcgc cgacaacggc
    11161 gtcaacccgc ttccggcgag cggatccact gccggcgcgg gcgccggcgg cacaacgacg
    11221 ccacccgccg gcggcggcac gggcgggacc ggcggatcgg gcggaacgac cactccgccc
    11281 ccggacgcga cttcaatcgt gcggtcctgg tacggaatac aggccaacat cctccagccg
    11341 ttctgcgtga cgtgccacca gggggctgcc cagttcgccg ccggcctgtc atgggaggtt
    11401 ggccagtacg aggcaattgt gacgaacggc cgtctgagca ccgagatcgc gacgatgctc
    11461 gaggtcaacc cgaacaaccc ggacgcgagc tacctcatgt ggaagctgcg cggacagggt
    11521 cccgctgggg agccaatcgt cggcgtgcgc atgcccgcga ccggcattgc actcgacccg
    11581 gcgctgatca cggcaatcca ggagtggatt gctgcgggcg ccccgcttgg cgacccggcg
    11641 gacgctactt cgggcggcag cactggcggc ggcggctcgg gcggaacgac caaccccccc
    11701 cctgacccca cggccatcgt cccgacctgg tacggagtac aggccaacat cctccagccg
    11761 ttctgcgtga cgtgccacca gggggctgcc cagttcgccg ccggcctgtc atgggaggtt
    11821 ggccagtacg aggcaattgt gacgaacg
//
LOCUS       DBNHKE010000148        15382 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_448499, whole genome shotgun sequence.
ACCESSION   DBNHKE010000148 DBNHKE010000000
VERSION     DBNHKE010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15382
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_448499"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /gene="glmS"
                     /locus_tag="AC16O9_09950"
     CDS             <1..1362
                     /gene="glmS"
                     /locus_tag="AC16O9_09950"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR6516979.1"
                     /translation="GAYAIAVVAKGEPHRVVGARAGSPLVVGVGAGEMFLASDALALA
                     GASERIAYLEEGDVAEIELSGFRVRDAADREVKREVRTVKANATRVELGPYRHYMQKE
                     IFEQPGAVADTLEGIETVTAAPFGKDAARLLRGAKSVLVLACGTSYYAGLTAKNWIES
                     LARLPTQVEIASEYRYRDSVPDKDALVVVVSQSGETADTLAALRHARKLGQKRTLAVC
                     NVATSAMVRETQLQFLTRAGIEIGVASTKAFTTQLAGLFLLALVLAKLRRRLPARAEK
                     QHLRRLRHLPKALAAALALEPQLMAWAERFAKKEHALFLGRGLHYPIALEGALKLKEI
                     SYIHAEAYPAGELKHGPLALVTAEMPVVTVAPNDALLEKVKSNMQEVRARGGELYVLA
                     DADTRIAPQEGMHVIRLPEHYGLLSPILHVVPLQLLAYHTALARGTDVDKPRNLAKSV
                     TVE"
     gene            1367..2917
                     /locus_tag="AC16O9_09955"
     CDS             1367..2917
                     /locus_tag="AC16O9_09955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6516980.1"
                     /translation="MSRYDAVIVGAGHNGLTCACYLARGGLKVKVLERRARVGGAAVT
                     EEFHPGFRNSVAAYTVSLLQANVIRELRLAEHGLRIVERPLSNFVPLEQGYLKVGGGL
                     EATQHEFAKFSARDAERLPAYWARLERVADVLRALALETPPNVGGGVRDALAALRTGW
                     RMKALDAVARRDLLGLFTRSAADWLEDWFESDPVKAVFGFDAVVGTYASPSHPGTAYV
                     LLHHCFGEVNGKRGVWGHAIGGMGAISEALAAEARRLGVEIECEAPVAKVQPGAVVLA
                     SGEEIAARAVVANVNPKRLFLAMVEASSLERDFRERIERYKCASASFRMNVALSALPK
                     FSCLPEAGPHHASGIIMAPSLDYMDRAYLDARATGMARAPIVEMVIASALDDTLAPKG
                     AHVASLFCQHFAPDADWATRKDEAIGQIFDVVESHAPGFRQSVIAHTALSPADLEAEF
                     GLVGGDIFHGQLSLDQLWLARPVLGYGDYRTPLPGLYLCGSGAHPGGGVTGAPGHNCA
                     REVLRDLK"
     gene            2994..3221
                     /locus_tag="AC16O9_09960"
                     /pseudo
     CDS             2994..3221
                     /locus_tag="AC16O9_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025700.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
     gene            3272..3631
                     /locus_tag="AC16O9_09965"
     CDS             3272..3631
                     /locus_tag="AC16O9_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516981.1"
                     /translation="MRSLLKTLAILLLVALMPLRAMAAVTIGFCPSGHEDMAVAAHAG
                     NGHAAGTHAHGTQGAASQSAELWCNICAEHCSSAAFAPSVDGAMTVRPVGNHRTQFAG
                     RFAPAPVPDPLDRPPLA"
     gene            3700..3969
                     /locus_tag="AC16O9_09970"
     CDS             3700..3969
                     /locus_tag="AC16O9_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516982.1"
                     /translation="MTQSHWLLLAALAAGTAVAQAPARPDPADPAARVAAPTYRSAFE
                     GYQPARSGEPKSWREANDETARIGGHIGILREQAAREKQTQGERK"
     gene            3966..5360
                     /locus_tag="AC16O9_09975"
     CDS             3966..5360
                     /locus_tag="AC16O9_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6516983.1"
                     /translation="MKALAILSLALLAGCATFSEDGGFGRVEQAAAERGAPGAKWVRS
                     AQDADSVGARVKELLAQPLTAENAVQIALLNNPGLQARYAELGIAEAELVQASRWSGP
                     KLSFARLTRGEDIEREASVFFDVLGLISIPLSTRAQEKRFAAAQHRATEEALSLALAT
                     RKAWLTAVAAQQTARYMEDVQLAAEASADLARRMAEVGNWPFLTRAREQAFYAETTAR
                     LARARQTEVAARERLTRLLGLWGEEIHYALSERLPELPAAAREADDLEGKALARRLDV
                     QAARRDAEGLAAALGLTKVTRYVDLFEVGALRSTETGEEVKRGWEVELRIPIFDFGGA
                     RVARAEHQYMRAVNRAADTAVRARSEVRESYSAYRTAFDLARHYRDEIVPLRKRISEE
                     MLLRYNGMLSSVFELLADARDAVASVNAYLEAQRDFWIAEADLQMALTTGSPGAAPAA
                     APTMAPAAGGAPAH"
     gene            5373..6746
                     /locus_tag="AC16O9_09980"
     CDS             5373..6746
                     /locus_tag="AC16O9_09980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR6516984.1"
                     /translation="MTTRRNFMRASAAGLFGAALVSRAGAATLPEAPLMDSPDMAPPL
                     FPPNGRPYNPVATLNGWTLPWRMKDGWKEFHLIAEPVRRQMAPGMTANLWGYNGQSPG
                     PTIECVEGDKVRIFVTNKLPEHTTIHWHGILLPNGMDGVGGLTQPQIPVGKTFVYEFQ
                     MKKSGTFMYHPHADEMVQMAMGMMGFLVVHPKDPKFMAVDRDFVFLLNAYDIDPGSFT
                     PRVNTMLDFNLWSWNSRVFPGIDPFVVRKGDRVRIRFGNLTMTNHPIHMHGYDFEVTC
                     TDGGWVPKSARWPEVTVDVAVGQMRAFEFVADAPGDWAIHCHKSHHVMNAMGHNVPTM
                     IGVDHRGIAQKIMKLVPDYMVMGERGMADMGEMEMPIPDNTLPMMTGAGPFGPIEMGG
                     MFSVVKVREGLARNDYKDPGWYEHPAGTVSYAWGGPAPSAEQAASAPAVQNVLQVRKP
                     PGHNGHR"
     gene            6756..7238
                     /locus_tag="AC16O9_09985"
     CDS             6756..7238
                     /locus_tag="AC16O9_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR6516985.1"
                     /translation="MKLIYLLLCIAISGAVHAHGEKAHAEPRFDYSKAEPTSFGIAAD
                     PRDAKRTIRVQMRDTMRFVPGEVSVRQGEVVRFVAENEGAILHEMVIGTKEELERHAA
                     LMRKFPGMEHDEPHMTHVKPGKTGDIGWRFTKPGTFYYGCLIPGHYEAGMIGEITVTP
                     "
     gene            7258..7584
                     /locus_tag="AC16O9_09990"
     CDS             7258..7584
                     /locus_tag="AC16O9_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HIR6516986.1"
                     /translation="MTTKTIAILAFSALAGLAPLHAGAQQGGMTHAQDSTLADGVVRK
                     VDKDAGKLTIKHGSIPSMDMPPMTMVYRVKDPAMLDKVKPGDEIKFQMEKIGGQYTVT
                     RIGAAK"
     gene            7677..8060
                     /locus_tag="AC16O9_09995"
     CDS             7677..8060
                     /locus_tag="AC16O9_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516987.1"
                     /translation="MLTSGLPLSRRVFLALAALALLAVLLRPACELWFAHAGVGSPSP
                     AAGAPIGHGGNPAVHCCVSVSDGQQLAPLQVVSGGLRAPEGGALAALAVVVAGIAVLT
                     RRQHWLRAPPRRPRSFYLRSARILR"
     gene            8155..8730
                     /locus_tag="AC16O9_10000"
     CDS             8155..8730
                     /locus_tag="AC16O9_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR6516988.1"
                     /translation="MEHQALATGQGAAPEPRRLYLWPLAIFVVIAIFLGIGLTLDPRQ
                     LPSPLINKPVPQFRLSPVQGRTLGLASEDLRGEVAVVNVFASWCKACRDEHPLWMELA
                     QKRALPIHGLNYKDQPEDAARWLNELGDPYTRTGADRDGRVGIDWGVYGVPETFVIDK
                     RGMIRDKIIGAITRESVDKRLLPLVRQLQAE"
     gene            8747..9307
                     /locus_tag="AC16O9_10005"
     CDS             8747..9307
                     /locus_tag="AC16O9_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR6516989.1"
                     /translation="MKRSAALQSSQSSNKGLIVGISLILLGLVVAIIAANPGAKPQLT
                     RSAEPAPPASAGARQAGALKAREARYEFGPISMAAGKVSHRYWFRNESGAPVLIRQVY
                     TSCMCTTATLVKGMRVIGSYGMPGHGPLPAVNQTLAPDEAAYVDVVFDPAAHGPAGLG
                     FTERVVTIEPGAGVPLQVAFTAFVKP"
     gene            9311..10093
                     /locus_tag="AC16O9_10010"
     CDS             9311..10093
                     /locus_tag="AC16O9_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516990.1"
                     /translation="MDRKLTVVVAAGILLASIIAMFQFGSAGTAALWSASEGGRWLLP
                     LVAVAALIDSVNPCAFSILLLTVAFLLSIGKLRSNVLQIGAAYIVGLFFVYLLIGLGL
                     LQTLHLFDTPHFMASVGAGLLVLLGLVNIANEYVPAFPVKLAIPQAAHHRMATLMQRA
                     SIPTAVALGGFVGVCEFPCTGGPYLMAVGLLHDQATYYAGVGYLLLYNVIFVLPLILI
                     LLVASNASLLGKVQQWQQNRRRAMRLGGGSAMIALGLLVFAI"
     gene            10098..10544
                     /locus_tag="AC16O9_10015"
     CDS             10098..10544
                     /locus_tag="AC16O9_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516991.1"
                     /translation="MTSLVLALVSMILLALLAWLAGRVSRVSVCPVCVGVAGTWLWML
                     AARLGGFAIDTAILAMLLGASVVGIAHWIENRLPVGRAPLLWKALALPAGFSAAYALV
                     VEQWIAAGGAIAALALLAALFLRPARAAPTDPAVVAQLEERMKKCC"
     gene            10615..11250
                     /locus_tag="AC16O9_10020"
     CDS             10615..11250
                     /locus_tag="AC16O9_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6516992.1"
                     /translation="MKRESITQATVVPAPGAQRVAAPPGQAYPWYVRLIFAAQRRKYG
                     AELEPARLWGRLPRAFLMLTLLYRSLDRRSSPLEPALRALVQVRISQINWCAFCVDLN
                     GAAALERAVAPEKLGALEQFEASPLYTEREKAALAYAEALTDPARRVDDACFSRLRAH
                     FDEQEVLELTALAAFQNLSSKFNAALGVQAQGYCARVGGSKVDADQPDVHG"
     gene            11289..11648
                     /locus_tag="AC16O9_10025"
     CDS             11289..11648
                     /locus_tag="AC16O9_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516993.1"
                     /translation="MKRILATAAVLFLLLRPVCDVLAAGPAHDEGGQNTHAAALQDAS
                     GGTAHEHNVPCCADIEDGAVTTLSEAVVAGNGKLAFSAPAWSPLRSGAARADARHPPG
                     IPIAAASFYARTARVRR"
     gene            11735..12223
                     /locus_tag="AC16O9_10030"
     CDS             11735..12223
                     /locus_tag="AC16O9_10030"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR6516994.1"
                     /translation="MKRKLLAASAAILLGTATAACAQPYGGMGPGMMGGYGGHGMGPG
                     MMGGYGGYGMGPGMMGGYGYGLDLSAEQRGKISEIQREIAQKHWDLMGRLHQQGGPLA
                     EAFGPGPIDEQAARKAYDAMADAHKQMFDLSLQARKRIEAVLTPEQREQMSRGWRGRG
                     PR"
     gene            12235..12474
                     /locus_tag="AC16O9_10035"
     CDS             12235..12474
                     /locus_tag="AC16O9_10035"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR6516995.1"
                     /translation="MYGDMMGWGGGWGWFGIVHMVLWWFLIVLGIVVLAKWLFGGGGG
                     RGAEGDRALTILRERYARGEIDKQEFDARRRDLGG"
     gene            12476..12886
                     /locus_tag="AC16O9_10040"
     CDS             12476..12886
                     /locus_tag="AC16O9_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516996.1"
                     /translation="MKVVQTLRKVLFVAAALAIAGAGFAAELGTRSSSAAGVTVKVTP
                     RGVVPGADSWGFEVVLDTHSGDLSDDLVGNSVLLDTKGTRLAPLAWEGSGPGGHHRKG
                     VLRFKPPAGATEAIELQIRRPGESAPRSFRWKLK"
     gene            12897..13358
                     /locus_tag="AC16O9_10045"
     CDS             12897..13358
                     /locus_tag="AC16O9_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516997.1"
                     /translation="MATTIAIARPAGFGVGAFGALLAVYFGALTLVSGWTFTWQQFSE
                     FWFYVLPLAAGFGLQVALFLKLRGVISRAKQTGTVVAASGATSTAAMVSCCAHYLTNV
                     APVLGATGLVALAAQFQVELFWVGLAFNAAGIAYVGTKLFQASRAHAHCVT"
     gene            13429..14265
                     /locus_tag="AC16O9_10050"
     CDS             13429..14265
                     /locus_tag="AC16O9_10050"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6516998.1"
                     /translation="MIEVPSSRIHDAGALPAALAALLIAGVSMPMSSPAHAAGGAGER
                     GLEIARQMKERDRGFGNSTATLKMVLIDAGGGEALRELRTKALEGRDDGDKLLAIFEA
                     PADVRGTVFLSHTHAERADDQWLYLPALKRVKRIAPANKSSPFMGSEFNYEDLASVEV
                     EKYTHAYRGDELYEGEARFLLERVPVDAESGYSRQRVWVDKARYVPLKIEYFDRRGDK
                     LKTLALREYRLYLGRYWRAHEARMENHQSGKRTILRWDDIRFRQGLGEQDFLPASMAR
                     AR"
     gene            14278..>15382
                     /locus_tag="AC16O9_10055"
     CDS             14278..>15382
                     /locus_tag="AC16O9_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6516999.1"
                     /translation="MRALFLIMVAFVLAFSARAQEWGGQLSTEYRAFAHAGLDPEQHR
                     GYASLVAQPELRAQSRDGEQLLAFTGFYRWDQHDRQRTHGDVRELLWFSVGRGFELRA
                     GIGKIFWGVAETQHLVDVVNQIDLVENPDREEKLGQPMLGATLLTRAGNWELYLLPYF
                     RERTFPGAEGRLRTVPRVDTKQDARYESSRGKRHVDAAARWTSTLGAWDLGLSYFRGT
                     NREPRLVPGLDVAGQPVLLPYYGQLRQAGLETQATLDRWLLKLEAVRRAEADATSRAA
                     TAGFEYTLPGVFGGPSDLGLLAEYLYDSRGRAAFTPYQDDVMLGVRWSANDAQGTQVL
                     ATVIADRRSAERAYSLEASRRLAANLSASLEAGA"
BASE COUNT         2409 a         5079 c         5440 g         2454 t
ORIGIN      
        1 ggcgcctacg ccatcgctgt ggtggcaaag ggcgagccac accgcgtggt cggcgcgcgc
       61 gccggcagcc cgctggtggt cggggtgggc gcgggcgaga tgttcctcgc ttccgacgcg
      121 ctggcgctcg ccggcgcgag cgagcgcatt gcatacctcg aagagggcga tgttgcggag
      181 atcgagctct ccggatttcg cgtgcgggac gccgccgacc gcgaggtcaa gcgcgaggtg
      241 cgcacggtca aggcgaacgc cacccgggtc gagctcggcc cgtaccggca ctacatgcag
      301 aaggagatct tcgagcagcc gggcgcggtg gcagacacgc tcgaaggcat tgagaccgta
      361 accgccgcgc ccttcggcaa ggacgcagcg cggctgctgc gcggcgcgaa aagcgtcctc
      421 gtgctcgcct gcggcacgag ctactacgcg ggcctgacgg caaagaactg gatcgagtcg
      481 ctcgcgcgcc tgccgacgca ggtggagatc gcgagcgagt accgctaccg cgacagcgtc
      541 ccggacaaag acgcgctggt ggtcgtcgtg tcgcagtcgg gcgagaccgc cgacacgctc
      601 gccgcgctgc gccacgcgcg caagctcggc cagaagcgca cgctcgccgt gtgcaacgtc
      661 gccaccagcg ccatggtgcg cgagacgcag ctgcagttcc tcacccgcgc aggcatcgag
      721 atcggtgtcg cctcgaccaa ggcgttcaca acgcagctcg cggggctctt tcttctcgcg
      781 ctggtgctgg ccaagctgcg ccggcggctg ccggcgcgcg ccgaaaagca gcacctgcgt
      841 cggctgcgcc acctgcccaa ggcgctcgcg gcggcgctcg cgctcgagcc gcagctgatg
      901 gcctgggccg agcgctttgc gaagaaggag cacgccctct tccttggccg cggcctgcac
      961 tacccgatcg cgctggaagg ggcgctgaag ctgaaggaga tctcctacat tcacgcggaa
     1021 gcctacccgg cgggcgagct caagcacgga ccgctcgcgc tggtcacggc cgagatgccg
     1081 gtggtgaccg tggcgccaaa cgacgcactg ctggagaagg tgaagtcgaa tatgcaggaa
     1141 gtgcgcgcgc gcggcggcga gctctacgtg ctggcggacg ccgacacgcg catcgcgcca
     1201 caggagggca tgcacgtcat ccggctgccc gagcactacg ggctgctctc gcccatcctg
     1261 cacgtcgtgc cgctgcagct gctcgcgtat cacacggcgc tcgcgcgcgg caccgacgtg
     1321 gataagccgc gcaacctcgc caagtccgtt accgtagagt agggcggtga gccgctacga
     1381 cgcggtgatc gtcggcgcgg ggcacaacgg cctcacttgc gcctgctacc tcgcgcgcgg
     1441 cggcctgaag gtgaaggtgc tcgagcgccg tgcgcgcgtc ggcggcgccg ccgtgaccga
     1501 ggagttccac cccggctttc gcaactcggt ggccgcctac accgtgagcc tgctgcaggc
     1561 gaacgtgatc cgcgagttgc ggctggcgga gcacggattg cgcatcgtcg agcggccgct
     1621 ctcgaatttc gtgccgctcg agcagggcta cctcaaggtg gggggcgggc tggaggcgac
     1681 gcagcacgag ttcgccaagt tctccgcgcg cgacgccgag cgcctgcccg cgtactgggc
     1741 gcggctggag cgcgtggccg acgtgctgcg cgcgctggcg ctggagaccc cgcccaatgt
     1801 cggcggcggc gtgcgcgatg cgctggcggc gctgcgcacg ggctggcgca tgaaggcgct
     1861 cgacgcggtc gcgcgacgtg atctccttgg cctgttcacc cgcagcgcgg ccgactggct
     1921 cgaggactgg ttcgaatccg atccggtgaa ggcggtgttc ggcttcgacg ccgttgtcgg
     1981 cacctacgcg agtcccagtc atcccggcac cgcgtacgtc ctcctgcacc actgctttgg
     2041 cgaggtgaac ggcaagcgcg gcgtctgggg ccacgccatt ggcggcatgg gcgcgatcag
     2101 cgaggcgctc gccgccgagg cgcggcgcct gggcgtcgag atcgagtgcg aggcgccggt
     2161 ggcgaaggtg cagccgggcg ccgtggtgct ggcgtccggc gaggagatcg ccgcgcgggc
     2221 ggtggtcgcc aacgtgaatc cgaagcgcct gttcctcgcg atggtcgagg cctcgtcgct
     2281 cgagcgcgac tttcgcgagc gcatcgagcg ctacaagtgc gcttcggcct cgttccgcat
     2341 gaatgtggcg ctctccgcgc tgccgaaatt ctcctgcctg cccgaggcag ggccgcacca
     2401 cgcctcgggc atcatcatgg cgccgtcgct cgactacatg gatcgcgcct atctcgatgc
     2461 gcgcgccacg ggcatggcgc gcgcgccgat cgtcgagatg gtgatcgcga gcgcgctcga
     2521 cgacacgctc gcaccgaaag gcgcgcacgt ggcgagcctg ttctgccagc actttgcgcc
     2581 cgatgcggac tgggccacgc gcaaggacga agccatcgga cagatcttcg acgtggtgga
     2641 gtcgcatgcg ccgggtttcc ggcaaagcgt cattgcccac acggcgcttt cgccggccga
     2701 cctcgaagcg gagttcgggc tcgtgggcgg cgacatcttc cacggccagc tctcgctcga
     2761 tcagctctgg ttggcgcggc cggtgctggg gtacggcgac taccgcacgc cgttgccggg
     2821 gctgtacctc tgcggctcgg gcgcgcaccc ggggggcggg gtcaccggtg cgccggggca
     2881 caattgcgcg cgcgaggtgc tgcgggacct caagtagcgc aggcgccttt cgcgccggaa
     2941 cgcggagacc gcgcccgcct tgactttccc atcatgggaa ggatgaagct gcgcggctcg
     3001 cgctcgccgc cgataccgcc tccatcacca tcatggagat cgtggacaac gcgatcatgc
     3061 tggcaatccc cggagcgatg caggctccgc tcgacagcgc gctgttctgg ggcagcctcg
     3121 caatcgcttt ggccctggcc gcggtcgcgg catttccggt gaaccgctgg ctgatcgcgc
     3181 gcggccgcgg tcacgcggtc gttcacgcgc atcatcgttg acgcacgcgc cgcgggaact
     3241 acgcatcgag gcctgtgtca taatgcattc gatgcgcagc ctgctgaaaa cgctcgcgat
     3301 tctgctcctt gtggcgctca tgcccttgcg cgcgatggcc gccgtgacca tcggtttctg
     3361 tccctccggc catgaagaca tggcggtcgc ggcgcatgcc gggaatggtc atgcggcggg
     3421 tacgcatgcg catggcacgc agggcgccgc gtcgcaatcc gcggagctct ggtgcaacat
     3481 ttgcgccgag cattgctcga gcgctgcgtt cgcgccatcg gtggacggcg ccatgacggt
     3541 tcggcccgtc ggcaaccacc gcacgcaatt cgccgggcgc ttcgccccgg cacccgttcc
     3601 cgacccgctc gaccgccccc cgctcgccta gcttcgctgc gacctgagac gccgcggccc
     3661 gttgccgcgc gcggttcatt cattgcgaag gaagggagca tgactcagag ccactggtta
     3721 ttgctcgcgg cattggctgc gggcaccgca gtagcacaag cgccggcgcg cccggatccc
     3781 gccgatccgg cagcgcgcgt cgccgcacca acctaccgct ccgcgttcga gggctaccag
     3841 ccggcccgaa gcggagagcc caagtcctgg cgcgaggcga acgacgagac cgcgcgcatc
     3901 ggcggtcata tcggcatcct gcgcgagcag gcggcgcgcg agaagcagac gcagggagag
     3961 cgcaaatgaa ggcgctcgcc atcctgtcgc tggcactgct cgccggctgc gcgacgttca
     4021 gcgaggacgg cggcttcggc cgcgtggagc aggcggcagc ggagcgcggc gcccccggtg
     4081 cgaaatgggt gcgctctgcc caggacgccg attcggtcgg cgcccgcgtc aaggaactgc
     4141 tcgcccagcc gctcacggct gagaacgcgg tgcagatcgc gttgctcaac aatccaggcc
     4201 tgcaagcgcg ctacgccgag ctcggcatcg ccgaggccga gctcgtccag gcaagccgct
     4261 ggagcggccc caagctcagc ttcgcccggc tgacccgcgg cgaggacatc gagcgcgagg
     4321 cgagcgtgtt tttcgatgtc ctcgggctga tctcgattcc gctttccacg cgggcacagg
     4381 agaagcgctt cgcggccgcc cagcaccgcg cgaccgagga ggcgctcagc ctcgcgcttg
     4441 ccacgcgcaa ggcctggctt accgccgtcg cggcgcagca gacggcgcgc tacatggagg
     4501 acgtgcagct cgccgccgag gcgagcgccg acctcgcacg gcgcatggcc gaggtcggca
     4561 actggccgtt cctcacccgc gcgcgcgagc aggcgttcta cgccgagacc accgcccggc
     4621 tcgcgcgcgc gcgccagacc gaagtggctg cgcgcgaaag gctcacccgc ctgctcgggc
     4681 tctggggcga ggagatccac tatgcgctct ccgagcggct gcccgagttg ccggcggccg
     4741 cgcgcgaggc ggacgatctc gagggcaagg cactggcccg acggctcgac gtgcaggcgg
     4801 cacggcgcga cgcggaaggc cttgccgccg cgctggggct gacgaaagtt acccgctacg
     4861 tcgacctgtt cgaagtgggc gcactgcgct cgaccgagac cggggaggag gtaaagcgcg
     4921 gctgggaagt ggagctgcgc attccgatct tcgacttcgg cggcgcgcgc gtggcgcgcg
     4981 ccgagcacca gtacatgcgg gcggtgaacc gcgccgcgga taccgcggtg cgggcgcgct
     5041 ccgaagtgcg cgagtcctac tcggcgtacc gcacggcgtt cgacctggcg cggcactacc
     5101 gcgacgagat cgtgccgctc aggaagcgca tctcggagga gatgctcctg cgctacaacg
     5161 gcatgctctc ctcggtgttc gagctgctcg cggacgcgcg cgacgcggtg gcgagcgtga
     5221 acgcgtacct cgaagcgcag cgcgacttct ggatcgcgga ggccgacctg cagatggcgc
     5281 tgaccaccgg ctcgccgggc gcggctccgg ctgccgcgcc gacgatggcg cctgccgccg
     5341 gcggcgcacc ggcgcactga aaggaatcga tgatgacgac acgcaggaac ttcatgcgcg
     5401 cctcggccgc cgggctgttt ggcgcggcgc tggtgtccag ggccggcgca gcgacgctgc
     5461 ccgaggcgcc gctcatggac tcgcccgaca tggcgccgcc gctctttccg ccgaacggcc
     5521 ggccctacaa cccggtggcg acgctgaacg gctggacgct gccctggcgc atgaaggacg
     5581 gctggaagga gtttcacctg atcgccgagc cggtgcgccg ccagatggcg cccggcatga
     5641 ccgcgaactt gtggggctac aacggccagt cgcccgggcc gacgatcgag tgcgtcgaag
     5701 gcgacaaggt gaggatcttc gtcaccaaca agctgcccga gcacactacc atccactggc
     5761 acgggatcct cctgcccaac ggcatggatg gcgtgggcgg gctcacgcag ccgcagatcc
     5821 cggtcggcaa gaccttcgtc tacgagttcc agatgaagaa gtcgggcacc ttcatgtacc
     5881 acccgcacgc cgacgagatg gtgcagatgg cgatgggcat gatggggttt ctcgtcgtgc
     5941 acccgaagga cccgaagttc atggcggtag accgcgactt cgtgttcctc ttgaacgcct
     6001 acgacatcga tccgggcagc tttacaccgc gcgtgaacac catgctcgac ttcaacctgt
     6061 ggtcatggaa ctcgcgcgtg tttccgggga tcgatccctt tgtggtgcgt aagggcgacc
     6121 gcgtgcgcat acgcttcggc aatctcacca tgaccaacca tcccatccat atgcacggat
     6181 acgacttcga ggtcacctgc accgacggcg gctgggtgcc gaagtcggcg cgctggcccg
     6241 aggtgaccgt ggacgtcgcc gtgggtcaga tgcgcgcctt cgagttcgtg gccgacgcgc
     6301 cgggcgactg ggcgatccac tgccacaagt cgcaccacgt gatgaatgcc atggggcaca
     6361 acgtgcccac catgatcggg gtcgaccacc gcggcatcgc acagaagatc atgaagctgg
     6421 tgcccgacta catggtgatg ggcgagcgcg gcatggccga catgggcgag atggaaatgc
     6481 ccatcccgga caacacgctg ccgatgatga ccggcgccgg gcctttcggc ccgatcgaga
     6541 tgggcggcat gttcagcgtg gtgaaagtgc gcgagggcct ggcgcgcaat gactacaagg
     6601 atccgggctg gtacgagcat cctgccggca ccgtgtctta tgcatggggc ggcccggcgc
     6661 cgagcgccga gcaggcagcg tccgcgcccg cggtgcagaa cgtgctccag gtacgcaagc
     6721 cgcccggcca taacggacat cgatgaggag agccgatgaa actgatctat ctgttgctgt
     6781 gcatcgctat ttccggcgca gtacatgcgc acggcgagaa ggcgcatgcc gaaccgagat
     6841 tcgattactc gaaggcggag cccacgtctt tcggcatcgc cgccgatccg cgcgacgcga
     6901 agcgcaccat ccgcgtgcaa atgcgcgaca cgatgcgctt cgttcccggc gaggtctcgg
     6961 tgcgtcaagg cgaggtggtg cgcttcgtgg ccgagaacga gggcgcaata ttgcacgaga
     7021 tggtgatcgg cacgaaggaa gagctcgagc ggcacgccgc gctgatgagg aagtttcccg
     7081 gcatggagca cgatgagccg cacatgacgc acgtcaagcc cggcaagacg ggcgacatcg
     7141 gctggcgctt caccaagccc ggcacgttct actacggctg cctgatcccc ggccactacg
     7201 aggcggggat gatcggagaa atcacggtta ccccctgaca tgcaaaagga aaacatcatg
     7261 acgacgaaga cgattgcgat tctcgcgttc agcgcccttg ccggcctggc gccgcttcac
     7321 gccggcgccc agcagggcgg catgacacac gcgcaggaca gcacactggc cgacggcgtg
     7381 gtccgcaagg tggacaagga cgcgggcaag ctcacgatca agcacgggtc gatcccgagc
     7441 atggacatgc cgccaatgac catggtgtac cgggtcaagg atccggcgat gctggataag
     7501 gtgaagccgg gtgacgaaat caagttccag atggaaaaga tcggcgggca gtacacggtg
     7561 acccggatcg gggccgcaaa gtagcctggc cgtgaaattt cgcaccccgc ggctcgctgc
     7621 gaggttgatc tgcgtcaagc acgttttcgt ggaggcgggc attctggcag ccggatatgc
     7681 tgactagcgg attgccgctt tctcgccgcg ttttcctggc gctcgccgcg ctggcgttgc
     7741 tcgccgtgct gctgcggccg gcgtgcgagt tgtggtttgc gcatgcgggc gtcggtagcc
     7801 cgtcccccgc cgcgggcgcg ccgatcgggc atggcggcaa tccggcggtt cattgctgcg
     7861 taagcgtcag cgatgggcag cagcttgccc cgttgcaggt cgtatccggc ggcttgcgcg
     7921 cgcccgaagg cggtgcgctc gcggcgctcg ccgtcgtggt tgccggcatc gccgtgctca
     7981 cacggcgcca gcactggctc cgcgcgcccc ctcgtcgtcc tcggtccttc tatctccgct
     8041 cggcgcgaat cctgcgctga gatcctcgat gcgcgggaac ccgggcgctg cgtgcgtccg
     8101 gcgtacgcat tgcgggctgc cgaatggcaa gcaacggctg ggaaggaatc gatcatggaa
     8161 caccaagcac tcgctaccgg gcaaggcgcg gcgcccgaac cgcgccggct gtatctgtgg
     8221 ccgctcgcga tcttcgtggt gatcgcgatc tttctcggca ttggactcac gctcgatccg
     8281 cgccagctgc cctcgccgct catcaacaag cccgtgccgc agttccggct ctcgccggtg
     8341 cagggacgca cgctcggcct ggcgagcgag gatctgcgcg gcgaggtcgc ggtggtcaac
     8401 gtgttcgcct cctggtgcaa agcctgccgc gacgagcacc cgctgtggat ggagctcgcg
     8461 caaaagcgcg ccctgccgat ccacggcctg aactacaagg accagcccga ggacgcggcg
     8521 cgctggctga acgagctggg cgacccgtac acgcgcaccg gggccgatcg ggacgggcgt
     8581 gtcggcatcg actggggcgt ctacggcgtg cccgaaactt tcgtcatcga caagcgcggc
     8641 atgatccgcg acaagatcat cggcgcgatc acccgcgagt cggtcgacaa gcgcctgctg
     8701 ccgctggtgc gccagctgca ggcggaatga acttgaagga ggtcttatga aacgcagtgc
     8761 tgccttgcaa tccagccagt cgagcaacaa aggactgatc gtgggcattt ccctgatcct
     8821 tctggggctc gtcgtcgcca tcatcgcggc gaatcccggt gccaaaccgc agctcacccg
     8881 gagtgcagaa ccggcgccgc cggcgagcgc gggggcgcgg caggccggcg cgctgaaggc
     8941 gcgcgaggcg cgttacgagt tcgggccgat ttccatggca gccggaaagg tgagccatcg
     9001 ctactggttc aggaacgaga gcggcgcccc ggtgctgatc cggcaggtct acacctcctg
     9061 catgtgcacg acggcgacgc tggtcaaggg catgcgggtg atcggcagct acggcatgcc
     9121 ggggcatggt cctctgccgg ccgtcaacca gacgctcgcg ccggacgagg cggcttacgt
     9181 ggacgtggtg ttcgatccgg ccgcgcatgg cccggcgggc ctgggattca ccgaacgcgt
     9241 ggtcacgatc gaacccggtg ccggcgtgcc gctgcaggtc gctttcaccg ccttcgtgaa
     9301 gccctagccc atggaccgaa aactgactgt cgtcgtcgcc gcggggattc tgctcgcttc
     9361 gatcatcgcc atgttccagt tcggcagtgc cggcacagcg gcactgtgga gcgcgagcga
     9421 gggcgggcgc tggctgctgc ccctggtcgc ggtggcggcg ctgatcgaca gcgtcaatcc
     9481 ctgcgcgttt tcgatcctgc tgcttacggt ggcgttcctg ctgagcatcg gcaaactgcg
     9541 ctcgaacgtg ctgcagatcg gcgccgccta catcgtcggg ctgttcttcg tgtacctgct
     9601 gatcgggctc gggctgctgc agacgctgca cctgttcgac acgccgcact tcatggcgag
     9661 cgtgggcgcg gggctgctgg tgctgctagg cctggttaat atcgccaacg agtacgtgcc
     9721 ggcatttccg gtcaagctgg cgattccgca ggcggcgcat cacaggatgg cgacgctcat
     9781 gcagcgggcc tcgattccga ccgccgtcgc gctgggcggg ttcgtgggcg tgtgcgaatt
     9841 cccctgcacc ggcgggccgt acctgatggc ggtcgggcta ttgcatgatc aggccacgta
     9901 ttacgccggt gtgggctatc tgctgctcta caacgtcatc ttcgtgctgc cgctgatcct
     9961 gatcctgctc gtggcgagca atgcgtcgct cctcggcaag gtgcagcagt ggcagcaaaa
    10021 caggcgcagg gcgatgcgcc tcgggggcgg cagcgcgatg atcgcgctgg gactgttggt
    10081 cttcgcgata tgacgccatg accagcctcg tcctggcgct cgtctcgatg atcctgctcg
    10141 ccttgctggc gtggctggcg ggccgggttt cgcgggtttc ggtgtgcccg gtgtgcgtcg
    10201 gcgtcgcggg gacctggctg tggatgctcg ccgcgcgcct gggcggcttc gccatcgaca
    10261 cggccattct cgccatgctc ctgggcgcgt cggtggtggg catcgcacac tggatcgaga
    10321 accgcctgcc agtggggcgc gcgccgttgc tctggaaggc gctcgccctg ccggccgggt
    10381 tcagcgcggc atacgcactg gtcgtcgagc agtggattgc cgctggcggg gcgatcgcgg
    10441 cgcttgcgct tctcgcggcc ttgttcctgc gcccggcgcg tgcggcgcca accgatcccg
    10501 ccgtggttgc gcaactcgaa gagcggatga agaaatgctg ctgagccgac atgtcgcgcc
    10561 gggaagcgag ggtggaggaa acgaaaggcg cgagcgggtg tccataggtc gggtatgaaa
    10621 cgcgaatcga tcacgcaagc gaccgttgtg ccggcgccag gcgcgcagcg cgtcgcggcc
    10681 ccacctggcc aggcctaccc ttggtatgtg cggctgatct ttgccgcgca gcggcgcaag
    10741 tacggcgccg agctcgagcc ggcgcggctc tggggccggc tgccgcgcgc gtttctgatg
    10801 ttgacgctgc tctatcgcag tctcgatcgt cgcagctcgc cgctcgagcc ggccttgcgc
    10861 gcgctggtgc aggtgcgcat ctcgcagatc aactggtgcg ctttttgcgt cgacctgaac
    10921 ggtgccgcgg cgctggagcg ggccgtcgcg cccgaaaagc tcggtgcgct cgagcaattc
    10981 gaagcctcgc cgctgtatac cgagcgcgag aaggccgcgc ttgcgtatgc cgaggcgctc
    11041 acggatccgg cgcgccgcgt ggacgacgcc tgcttttcgc ggctgcgcgc gcatttcgac
    11101 gagcaggagg tgctggaact cacggcgctc gccgcgttcc agaacctatc cagcaagttc
    11161 aacgcggcgc tcggcgtcca ggcccaggga tattgcgcgc gcgtcggcgg gagcaaggtt
    11221 gacgcagatc aacctgacgt gcacggatga gagcagcatg gtcgcgcgat ggaaagcctg
    11281 atagtggcat gaagcggatt cttgccacgg ctgcggtgct gttcctgctg ttgcgccccg
    11341 tatgcgacgt gctggcggct ggcccggcgc atgacgaggg cgggcagaac acgcacgcgg
    11401 ccgcgctcca ggacgcaagt ggcgggactg ctcacgagca caatgtgccg tgctgcgcgg
    11461 acatcgaaga cggcgccgtg accacgttga gcgaagccgt tgttgcgggt aacggcaagc
    11521 tcgcgttttc ggcgcccgcc tggtccccat tgcgctcggg tgccgcgcgc gccgatgccc
    11581 ggcacccgcc cggcattccc atcgcagccg cttctttcta cgcacgcacc gcgcgcgttc
    11641 ggcgctgagt cgcggttttc ctgattcacc gagccgttgc ggcgggcaga ccgcccggcg
    11701 ctacctacta atccgttaac gaatggagtc gaaaatgaaa cgcaaattgc tggccgcttc
    11761 ggcggcgatc ctgcttggca cggccacagc cgcgtgcgcc cagccctacg ggggcatggg
    11821 ccccgggatg atgggcggct acggtggaca tggcatgggc ccgggcatga tgggcggcta
    11881 cggcggctac ggcatgggcc cgggaatgat gggtggctac ggctacggcc tcgacctcag
    11941 cgccgagcag cgcggcaaga tctccgagat ccagcgggag atcgcgcaga agcactggga
    12001 tctgatgggc cggctgcatc agcaaggcgg accgttggca gaggcgttcg gccccggacc
    12061 gatcgatgag caggccgcgc gcaaggccta cgacgcgatg gccgatgcgc acaagcagat
    12121 gttcgatctg tcgctgcagg cgcgtaagcg catcgaggcg gttctcacgc cggagcagcg
    12181 cgagcagatg agtcgcggct ggcgcggccg cggtccgcgc tgaggaggtc gattatgtac
    12241 ggcgacatga tgggctgggg cggcggctgg ggctggttcg gcatcgtgca catggtgctg
    12301 tggtggttcc tgattgtcct cggcatcgtg gtgcttgcga agtggctctt cggcggcggc
    12361 ggcggccgcg gagcggaggg cgaccgcgcg ctcacgatcc tgcgcgagcg ctacgcgcgc
    12421 ggcgagatcg acaagcagga attcgacgcg cgccggcgcg acctcggagg ttgacatgaa
    12481 agtcgtgcag acacttcgca aggtgctttt cgtggcggcg gcgctcgcga tcgcgggcgc
    12541 tggctttgcg gccgagctcg ggacgcgctc gagcagtgcg gctggggtga ccgtgaaagt
    12601 cacgccgagg ggcgtcgtgc cgggcgcaga cagttggggc ttcgaggtcg tcctcgatac
    12661 ccacagcggg gatctgagcg acgatctcgt cggcaattcc gtgctgctcg acacgaaagg
    12721 tacgaggctg gcgccgctcg cgtgggaggg ctcgggcccc ggcggccatc atcgcaaggg
    12781 cgtgctgcgc ttcaagccac cggccggtgc gacggaggca atcgagctgc agatccgccg
    12841 gccgggcgag agcgcgccgc gcagcttccg ctggaaattg aaataggacg agattcatgg
    12901 ccacgactat cgcaatcgcg cgacccgccg gctttggtgt cggcgccttc ggcgcgctgc
    12961 tggcggtcta cttcggcgca cttacgctag tgtcgggctg gaccttcacc tggcagcaat
    13021 tttccgagtt ctggttctac gtcttgccac ttgccgccgg cttcggtctg caggtcgcgt
    13081 tattccttaa gctgcgcggg gtgatcagcc gcgcgaagca gaccggaacc gtggtcgcgg
    13141 cttcgggggc aacttcgacc gcggcgatgg tctcgtgctg cgcgcattac ctgacgaacg
    13201 tcgcgccggt gctcggtgcg acggggctgg tcgcgctcgc agcgcagttc caggtggagc
    13261 tgttctgggt cgggctcgcg ttcaacgccg cgggcatcgc ctacgtgggt acgaaactgt
    13321 tccaggcgtc cagggcacac gcgcactgcg tgacgtaacg tctcgcgggg cttgaccttc
    13381 ccattatggc agggtgcagc ctgcacttgc catgcaagga agagccccgt gatcgaagta
    13441 cccagctcga ggatccacga cgcaggcgcg ttgccggcag cgctggccgc gctgctcatc
    13501 gcgggcgtat cgatgccgat gtcctcgccg gcgcatgccg cggggggcgc cggcgaacgc
    13561 ggcctggaga tcgcgcgcca gatgaaggag cgagatcgcg gcttcggcaa ttccaccgcc
    13621 acgctcaaga tggtcctgat cgacgccgga ggcggcgagg cgttgcgcga gctgcgcacc
    13681 aaggcgctcg agggccgcga cgacggcgac aagctgctcg cgatcttcga ggcgccggcg
    13741 gacgtgcgcg gcacggtgtt tctcagccac acgcacgccg agcgcgccga cgaccagtgg
    13801 ctctacctgc ccgcgctcaa gcgcgtgaag cgcatcgcgc cggccaacaa gagctcgcct
    13861 ttcatgggct ccgagttcaa ctacgaggat ctggcctcgg tcgaggtgga gaagtacacc
    13921 cacgcctacc gcggcgacga gctttacgag ggcgaggcgc gcttcctact cgagcgcgtc
    13981 ccggtcgacg ccgagtcggg ctacagccgc cagcgcgtct gggtggacaa ggcgcgctac
    14041 gtgcccctca agatcgagta cttcgaccgg cgcggcgaca agctgaagac gctcgcattg
    14101 cgcgagtacc ggctgtatct cgggcgctac tggcgagcgc acgaggcgcg catggagaac
    14161 catcagagcg gcaagcgcac gatcctgcgc tgggacgaca tccgcttccg gcagggcctg
    14221 ggcgagcagg attttctgcc ggcgagcatg gcgcgcgcga gatgagcggg acggcccatg
    14281 cgagcgctct tcctgatcat ggtcgccttc gtgctggcgt tttccgcccg ggcgcaggaa
    14341 tggggcgggc agctatcgac ggagtaccgc gccttcgcgc acgccggcct cgatccggaa
    14401 cagcaccgcg gctacgcgtc gctcgtggcc cagccggaac tccgagcgca gtcgcgggac
    14461 ggggagcagc tgctggcatt tacgggtttc taccgctggg atcagcacga ccggcagcgc
    14521 acgcacggcg acgtgcgcga gctgctgtgg ttcagcgtgg gtcgcggctt cgagctgcgc
    14581 gccggcatcg gcaagatctt ctggggcgtc gccgagacgc agcacctcgt cgacgtggtg
    14641 aaccagatcg acttggtgga gaaccccgat cgcgaggaaa agctcggcca gccgatgctc
    14701 ggcgcgacgc tgctcacgcg agcgggcaac tgggagctgt acctgctgcc gtacttccgc
    14761 gagcgcactt ttccgggcgc cgaagggcgg ctgcgcacgg tgccgcgcgt ggacacgaag
    14821 caggatgcgc ggtacgaaag ctcgcgcggc aagcgccacg tggacgccgc ggcgcgctgg
    14881 acgagcacgc tcggcgcgtg ggacctcggc ctgtcctact tccgcgggac caaccgcgag
    14941 ccgcggctcg ttccggggct cgacgttgcc ggccagccgg tcctgcttcc ttactacggg
    15001 caactgcgtc aggccggcct cgaaacgcag gccacgctcg atcgctggct gctgaagctc
    15061 gaggcggtgc gccgcgccga ggccgatgca acctcgcgcg ccgccaccgc ggggttcgag
    15121 tacacgctgc ccggcgtatt cgggggtccg tccgacctgg ggttgctggc cgagtatctg
    15181 tacgacagcc gcggccgcgc ggcctttacc ccgtatcagg acgacgtgat gctgggcgtg
    15241 cgctggagcg cgaacgacgc ccagggcacg caggttctcg caacggtcat cgcggaccgg
    15301 cgctccgccg agcgtgccta cagcctcgag gcaagccggc ggcttgccgc aaacctcagt
    15361 gcgagcctgg aagcgggggc gg
//
LOCUS       DBNHKE010000149        23592 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_448604, whole genome shotgun sequence.
ACCESSION   DBNHKE010000149 DBNHKE010000000
VERSION     DBNHKE010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 23592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23592
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_448604"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC16O9_10060"
     CDS             <1..380
                     /locus_tag="AC16O9_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517000.1"
                     /translation="ARKEGIIPGAANLRLPRFVGPRVARQAILAGRRLDCASPEGRRI
                     CDEIVPETGMDPAIDSLIALLTDSGVVSAAGNRRALRSGEEPLEQFRRYMAVYAREQA
                     VCHFSPALIANLEKHWDAANRRG"
     gene            complement(382..1179)
                     /locus_tag="AC16O9_10065"
     CDS             complement(382..1179)
                     /locus_tag="AC16O9_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517001.1"
                     /translation="MAYLEWGEAHNPDVVICVHGLTRCARDFDNLARALCGRYRVVCP
                     DVAGRGDSEWLADAMLYQIPQYVSDMVTLVARLDVERVQWVGTSMGGLIGMALAAQKG
                     SPVAKLVLNDAGPVVAKAALERIGQYVGQSPSFPSIEAAEKVMRAVSAPFGPHTDAQW
                     RFLTEVVLRKNTDGSWRLHYDPKIAEPYRKHMPDKDLEMWELWDAVACPTLVIRGAQS
                     DLLTRETAQAMTERGPRAKLVELPGIGHAPTLLHADQIAVVREFLLA"
     gene            complement(1236..1547)
                     /locus_tag="AC16O9_10070"
     CDS             complement(1236..1547)
                     /locus_tag="AC16O9_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517002.1"
                     /translation="MAFTAVVRVTGAAGRFEEFRERVRFLMVRDMDAENYTEHHRPGE
                     LEYRFEIARGIPFPAFVEATRAFEELRVEVEWDKDGARGGAAIEAGKLVDKWTGDRGP
                     A"
     gene            complement(1547..4102)
                     /locus_tag="AC16O9_10075"
     CDS             complement(1547..4102)
                     /locus_tag="AC16O9_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR6517003.1"
                     /translation="MTAAALAFTAGVLLLQQQAALPALAWLGALPACAALAAWRRALV
                     VPAAFAAGFLWAAGAAHLRLADRLAPELEGKDVEVAGVVSGLPAAAERGLRFEFEPES
                     STARLPGRVLLFWYEDLAGEPPPELQPGERWLLTLRLKRPHGPLNPHGFDYEAWLTER
                     GIGATGYVRAPQSARRLGARNSFVDRIAQARAAVRVRFHAVLGATPAAGILSALAVGE
                     QRAIANEEWRLFSRTGVTHLMSISGLHVTLVSGLAAWLASALWRRVPRLALALPARKA
                     GAATAIAAAFAYTLLAGFAVPAQRTFYMVSVVALALWSGRIASPARTLALALALVCAL
                     DPWAVLQPGFWLSFGAVALIFHVGTGWTQPEPRWRQWLRVQWAITVGLAPAALILFAQ
                     VSVAGPLANALAIPLVSAVITPLALAAAVLPVDALLHAAQWLVQWLLAYLEWCAALPG
                     ALWQQHVPPLWATLTALAGVAWLLMPRGFPWRGAGLALMLPLVALPPPAPAPGEAWVT
                     TFDVGQGLAVLVRTERHALLYDAGPAYGETDSGERIVAPALRALGVARLDALVLTHDD
                     SDHVGGARSILEGFEADALLHSLPDGHPLLALAPGPKRCQRGTSWTWDGVRFEILHPA
                     PGRTARRRNDESCVLKVSTDGSAMLLTGDIERGTELALLRDGVVRADALLVPHHGSRS
                     SSSAPFVDAVRPRLAVASAGYRNRFGHPAAEVRARYAAAGAQLLRTDRDGALTLRLSP
                     AGLAAERERARRARYWHVPAPPAYTGPAGVLPSNFQEAHMAKGDNAVYRVTDVIGTSP
                     NSWEEAAKNAVKTAASSLRDLRIAEVTKLDVKVEDGKVTQFRARLSLSFKYGG"
     gene            4139..4690
                     /locus_tag="AC16O9_10080"
     CDS             4139..4690
                     /locus_tag="AC16O9_10080"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR6517004.1"
                     /translation="MPRKFFRKYLPSADEVRGNRLAALFGKLLHDPNLWHLNRDSVAG
                     AVAIGLFAGLVPGPLQMLAALALAIPFRRNLPVALLTTFYTNPLTIVPLYVLAYGYGS
                     LLLGRDVRRLPVEPFEMDWANFFDSMARLADWTLALGKPLALGLVALALTLAVLGYLL
                     VQLGWRAYVVLAWRARREKRRRR"
     gene            4687..4989
                     /locus_tag="AC16O9_10085"
     CDS             4687..4989
                     /locus_tag="AC16O9_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517005.1"
                     /translation="MTHAEFVAAYREGRLKVQVDREAAARHVSARLMLPVVLLPLLGI
                     AVALALVGHYVIGGLVFAAALGLRFAVRRTAPGFVLQRALADEPFYAEAVASRLLK"
     gene            complement(5056..5727)
                     /locus_tag="AC16O9_10090"
     CDS             complement(5056..5727)
                     /locus_tag="AC16O9_10090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517006.1"
                     /translation="MSDAVLACAGLKKTYQGPAPVEVLRGVDFAVRARERVAIMGKSG
                     SGKSTLLHLLGGLDVPSAGTVQVRGRPFAEMDEAERGRVRNETLGFVYQFHHLLPEFT
                     ALENVAMPLFIRRMARGAALERAQAALAEIGLAARVEHLPGELSGGERQRAAFARALV
                     TEPACVLADEPTGNLDSNTADEVFEAMLKLSAARGTAFVIVTHDTALAGRAERVLHLD
                     NGTLK"
     gene            complement(5720..6961)
                     /locus_tag="AC16O9_10095"
     CDS             complement(5720..6961)
                     /locus_tag="AC16O9_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR6517007.1"
                     /translation="MRYELLVGLRYTRAKRRNHFISFISLISMAGIGLGVAALIVVLS
                     VMNGFQEEIRTRILGVASHVQVYDPRGGLADWEGVAQEVAALPRVQASAPYVHGQAMV
                     TVSGAARGVLVRGILPGPESRVTDIAQHMRAGSLEALRADEFGVVIGGDLARALRTYI
                     DDKLVLIIPRGSVTPAGVIPRLKQFTVVGIFDVGYAEADAALALIHLADAQRLYQIGD
                     AVSGVRLKLDDPLEARGVAREITLRSERGLEASDWTRANPNLFRAIEIEKRMIFIIMV
                     LIITVAAFNVVSTLVMLVTDKQADIAILRTLGAAPRSVMQIFIVQGSVIGVIGTLLGF
                     AGGLALAFNVDVVVPALETVLGFKFLSKDVYQIPDLPSKVEFMDVAIVCAVSLVLSLV
                     ATLYPSWRASRVNPAEALRYE"
     gene            6991..7452
                     /locus_tag="AC16O9_10100"
     CDS             6991..7452
                     /locus_tag="AC16O9_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025252.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6517008.1"
                     /translation="MNPDDAAALARLLRTGRVAHLGTLRQGAPLASMTLYLAAPDFSA
                     CYVHVSRLAWHTQDMQADPRVALSVSETDDGREDPFTLARVTIRGVAEALGAEPGLTQ
                     AWLARFPAQAVNFELGDFSFWRIVPHDARFVAGFGRIHNLSAAELAAAARV"
     gene            7449..7919
                     /locus_tag="AC16O9_10105"
     CDS             7449..7919
                     /locus_tag="AC16O9_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517009.1"
                     /translation="MSALLALALAAAPCAVEPEWRNEGVVQLLPACAPGYTPTLEGVR
                     TALRQAGRRGELVVGFGRVAHYPWLSSFLARQASASRHWPEERAAPEGYVTRALMGMP
                     EFTALFDPAWRIASIVVQDVRTKPAAELDLPQGHPLPPDFLLPYDAELVVTLVR"
     gene            7921..8520
                     /locus_tag="AC16O9_10110"
     CDS             7921..8520
                     /locus_tag="AC16O9_10110"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6517010.1"
                     /translation="MVTVYGMADSGNCYKVKLALEQLKLPYQWIEVSTTRGETRTREF
                     LARNPNGQVPTLQLEDGAYLPESGAILWYLAEGTPLLPAERLARARVLQWMFFEQYSH
                     EPCIAVARSILRYQPPDSPRRAELPRLQERGHKALGVMAQQLEKSDFFAGSKYSIADI
                     ALYAYTHCAADGGFELKRYPAVLAWLMRVKSQPGHVPLG"
     gene            8580..9971
                     /locus_tag="AC16O9_10115"
     CDS             8580..9971
                     /locus_tag="AC16O9_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6517011.1"
                     /translation="MLADTPLAIVDLETTGAHPLHDRVTEVAVIEVEHGEVRDEWSTL
                     VNPECAIPGAIQALTGITNDMVAGAPTFARLAQELHERLEGRVLVAHNARFDYGFLRQ
                     EFQRVGLAFRAKTLCTVKLSRRLYPEHARHNLDSLIARHNLDCRARHRALGDAEAVWQ
                     FLRLAAEERGEEVLAVAARQVAKQPSLPAHLDRAMVDAIPEAPGVYLLYGESGAPLYV
                     GKSIDMRARVLSHFADDLRSPREMQLAREVRRVEWQRTAGELGALLREAQLVKELLPV
                     FNRQLRRPADLCGFAFDAKRLRLVHDFDAEGIAHVHGLFRSRRAALAALRGLADAHGL
                     CLQSLGFETARKGACFRHQIRRCAGLCAGQESVHAHHARLATALAGLKNAAWPWRGPV
                     GVVEEDAAREATEVHVVHQWCYVGTARAEDEVPALLESARRPRFDLDQYKILQRHLRA
                     RRARVVELACTSS"
     gene            9956..10876
                     /locus_tag="AC16O9_10120"
     CDS             9956..10876
                     /locus_tag="AC16O9_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517012.1"
                     /translation="MHVELMVPGVFAAPPGPRRPALELLLARARVVHADPESAETWLG
                     RGFGQEQLPAGALTAGAPGFWLRADPVHLRVLRDRVVIVPLAGLAPETAAALVATLNR
                     HFAGRHEFRAPHPDRWVMSNAPEALRSSPPAPLRAPPARALAGADFGAHLPAAPWPAL
                     LNEIQMALHEHPANEGRELEVNGVWLWGAGELPATATAPWRSVSAEDPLALGLARIAR
                     LAHRTPPASATEWLAELPEEGRHLAAFDALHESLEADWFAPLVAALRAGRIGMLTLHV
                     PDAGCAFEAVRADLRRFWRRPRPLQAHAPR"
     gene            10863..12563
                     /gene="recJ"
                     /locus_tag="AC16O9_10125"
     CDS             10863..12563
                     /gene="recJ"
                     /locus_tag="AC16O9_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR6517013.1"
                     /translation="MRLVERPYDEVDRRALINAGLHPVLARVYAARRIRSAAELEDDV
                     KRLLPPASLARCGEAAVLLADAIAAQKKLLIIADYDADGATACAVGVRALRAMGASVD
                     YLVPNRFELGYGLTPELVDIAAARKPDLLITVDNGIASVEGVARASALGIATLVTDHH
                     LPGAELPRAACIVNPTQPGCGFASKAIAGVGVMFYVMLALRAELRTRGAFKDGKEPNL
                     AALTDLVALGTVADVVPLDANNRILVAEGLKRIRAGRAQPGLGELLRAAGRGAAQAGG
                     FDLGFILGPRLNAAGRLADMSLGIECLVTDDAARAANCAQELDRLNRERRRIEAEMLE
                     QARAAADAIAEAPGAAVALYDPAWHPGVVGILASRLRERLHRPVICFARANETAQSGQ
                     LRGSGRSIPGFHLRDCLDLVAKRDPGLIVRFGGHAQAAGLTLRETDLSRFGDAFARAA
                     EETMPREALTRTVETDGALEPQQFSLGLAQLLEARVWGPGFPPPLFCDTFALESQRVV
                     GEKHSKLQLVKDGRRLQAMRFNALDPLPTRFRAAYRLSVNEYNGVQSVQVSLEHVEGA
                     "
     gene            12560..13357
                     /locus_tag="AC16O9_10130"
     CDS             12560..13357
                     /locus_tag="AC16O9_10130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6517014.1"
                     /translation="MRLLALLALAPLLVLAQDKPVDAGPYVPSPQSVVSDMLKLAEVG
                     PEDFVIDLGSGDGRIVRTAALIFGARGFGVEIRDELVRQSNADAAREGIADRVKFVNA
                     DLFKTDVSQATVLTMYLLPDTVNLLREKLLRELKPGTRVLSHDYPLAGWIPEKYVQMD
                     LEDKVKISGVTTTLIYLYVVPAQVGGRWQARGPGGALTLDLKQQVTRVSGSARHAGRD
                     SALQDVKLRGTELSFGLGGKTWRGRVDGASIAGEIEGGGAWSARRGK"
     gene            13357..13566
                     /locus_tag="AC16O9_10135"
     CDS             13357..13566
                     /locus_tag="AC16O9_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HIR6517015.1"
                     /translation="MERKRVNSSKVRAVGYDERSQTLEVELSNGQVWQYSKVYPEVYR
                     RFMAAPNPATFYEDKIAEDYTGRRV"
     gene            13622..13924
                     /locus_tag="AC16O9_10140"
     CDS             13622..13924
                     /locus_tag="AC16O9_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517016.1"
                     /translation="MQRGADCNLRPDGSLGCRYRVGKDLEFELHRIGERGVGLRLTRS
                     SEAGDYRADREMMSRCVFVRHGERGREAGGGEFDYAFVSGRNGFVYRSLRECRLAR"
     gene            13921..15912
                     /gene="hyfB"
                     /locus_tag="AC16O9_10145"
     CDS             13921..15912
                     /gene="hyfB"
                     /locus_tag="AC16O9_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HIR6517017.1"
                     /translation="MSAQLVAVLVLAAAWLAIGAAGLAALHRLHLVARWLFPAGGAVA
                     LLIAAAALPALWGAPQAVVLPIGLPGAPFHFRLDGLSAFFLIVLGGAAAGVSLFSAGY
                     FRSREGTAPGLLCLQYHVFLAAMALVILADDAYAFMVMWETMALASFFLVITNHRLPE
                     IRRAAYLYLLIAHIGALAILLCFGVLQANTGDTSFAAMRAQTPTPFWASVAFLLALAG
                     FGAKAGLVPLHVWLPEAHPAAPSPVSALMSGVMLKTAVYGFLRVAFDLLPAQLWWWGV
                     LALALGLASAVFGVAFAAVQSDMKRLLAWSSVENLGLIFAGVGLALLFAAYRMPAAAA
                     LALTAALYHVASHAFFKSLLFVATGAVLHATSERSLGRLGGLLRVMPWVGWACLAGVL
                     ASAGLPPLGGFVSEWLLLQSFLFTPGLPNPFLNMLVPAVAAVIALVAALAGYVMVKFY
                     GVIFLGRHREDKLAAARDAGSWERLGMGWLVAGCVALGLFPALGIGLLAPVTELLVGA
                     RLPEAGGWLLVPVSAARASYGPLIFFAGVAASVGLAFVLVRRLYHGRLRRAAPWDCGF
                     PWQTARMQDTAEGFGQPVRQIFAPLYRMRRRLPSAFDAEPRYAVEVGDHFWHWLYLPL
                     GRLVESTARVIGTLQRGRIALYLVYSFATVIALLVWVTR"
     gene            15909..16865
                     /locus_tag="AC16O9_10150"
     CDS             15909..16865
                     /locus_tag="AC16O9_10150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR6517018.1"
                     /translation="MSALGLASQALEIVAALALAPLFAGWVAQCRAWLQSRTAPGLLQ
                     PYRVLHKLFNKDSVLAAHASPLFRIAPYVIFGAMTLACGIVPTLSTELPLSPAADAIA
                     LVGLFALARVFAALGAMDVGTAFGSLGARREMLVGFLAEPALLMVFFTAALISDSTSL
                     TTIVRTLSAREIGIQPSLALAAVALVMVALAENARVPVDNPATHLELTMVHEALILEY
                     SGRHLALLEWAAALKLFAYACIGMALFAPWGLAHAQAPLALVTALPVLVAKLAAAGLL
                     LALLETVSAKMRIFRVPEFLGMAFMLAVIAMLVKILLGGSAP"
     gene            16862..17521
                     /locus_tag="AC16O9_10155"
     CDS             16862..17521
                     /locus_tag="AC16O9_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HIR6517019.1"
                     /translation="MTGLMPQLVNLFAALLLLLAFAMLAQRRILSLIHLYTLQGVVLV
                     AAAAVMGYATRQPHLYVSAALTLALKVLLIPWLLHRVIDRLNVRWDVEMLINVPTTML
                     AGIMLVIFAFYLAAPISALAPSIARGTLGIALACVLLSFMMMITRSKAVPQVIGFLAM
                     ENALFFAATAATYGMPMVVELGIALDVLVGILILGVFLFQIREQFDSLDIRHLEKLKD
                     D"
     gene            17514..18977
                     /locus_tag="AC16O9_10160"
     CDS             17514..18977
                     /locus_tag="AC16O9_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HIR6517020.1"
                     /translation="MTSAVALGFLLGLPLAGALALALAGHRRRAPEANIAFSAGTLLA
                     AGVLTARVAAAGPVFVLGRAFYIDALNVFLVALTAFVALTTAIFSRPYMRVEQEHGRM
                     NPRRLRLYHSMYQLFSFTMLLALTTNNMGLLWVALEAAMLATVLLVSVYRSAASLEAA
                     WKYFILCGVGIAQALFGTVLLYMAAERAGAGEHGTLLWTHLDAVKASLDPRIMALAFA
                     FLFVGYGTKVGLVPVHNWLPDAHAEGPTPVSAVLSGLLLNVALYAVLRCKVLADGALG
                     PGLAGQMMMAFGLISVVVAAFFLSRQRDVKRMFAYSSIEHMGLITFAFGVGGPIATFA
                     GLLHMTVHSLVKSAIFFAIGHAAQHAGTQVMSEIRGLVKANPSVGWGLMLGSLAILGM
                     PPFGVFASEFLILTTTMREQPWAAPFLLAALGVAFASVLQRVQPMVFGETELKPLPQP
                     PALIPVFVHIGLVLVLGLYIPPSLEGWYREAARMVAG"
     gene            18974..20455
                     /locus_tag="AC16O9_10165"
     CDS             18974..20455
                     /locus_tag="AC16O9_10165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR6517021.1"
                     /translation="MRREVLKREHWVSAARAVVERGGRLVALWGSERREAYFVCAAYA
                     TPEGLVWSELPLDGGATYPDLSPVFYFAGRMQRALADLLGLTAEEALDRRPWLNHGAW
                     PYGYHPLLSNSTGKEVFDDCDVTDYPFVHVQGEGVHEIPVGPVHAGIIEPGHFRFSIV
                     GEKVLRLEKRLGYTHKGIEKRFTQLPPEGAHLLAGRVSGDSTVACAWAYCMALENAAD
                     AEVPERASWLRALLLERERVANHLGDLGALGNDAGFAFALTQFSRLREQWQRASREVF
                     GHRFMMDRVVPGGVAEDVNGAALELMREDCAAFAAEVKALRVIFDEHAGLQDRFIAAG
                     RVRPELANRLGVTGLVARASGQAIDLRNDHPCRPYDELGVQMATHRNGDVAARVIVRF
                     EELLESLRLIVEICNRLPEGAVRGEVEIPVGDAYGAGWVEGWRGEVFCGLELENGRIR
                     RCHLHDPSWQNWPALEHAIIDNIVPDFPLINKSFNLSYSGHDL"
     gene            20455..20961
                     /locus_tag="AC16O9_10170"
     CDS             20455..20961
                     /locus_tag="AC16O9_10170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR6517022.1"
                     /translation="MLKTVIKIVRTGILTEPAPTVHSLPEVSRIHHELLHILGRALCI
                     RQVDAGSCNGCELEIHALNNPYYNLEGYGIKFVASPRHADLLMVTGPVSRNMELALKR
                     TYEAIPDPKLVLAVGDCGCDGGIFGESYASCGRVSNVVPVDLTVAGCPPPPLEILRAV
                     LGLARRLR"
     gene            21144..21422
                     /locus_tag="AC16O9_10175"
     CDS             21144..21422
                     /locus_tag="AC16O9_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517023.1"
                     /translation="MMAPLSAPASERWFLMSRHGDCAPVATLKRKVPDLGEINDPVAF
                     AAFMRRKGVAVTSTRVPVPKGRAEEVKVPERHLFLMFVTSQMCRGAAR"
     gene            21683..21943
                     /locus_tag="AC16O9_10180"
     CDS             21683..21943
                     /locus_tag="AC16O9_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517024.1"
                     /translation="MTDPAAEKTPLERSRDIIKQLKEMLHYSEGNIEKLTGIWLLLDG
                     ELKQKRVAKGLEALLTHQNKFHDALAALIKDYESECDKLEKK"
     gene            21919..22227
                     /locus_tag="AC16O9_10185"
     CDS             21919..22227
                     /locus_tag="AC16O9_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6517025.1"
                     /translation="MRQAGEEIALIQCRHDPGVFVPVIDRNRCEGKARCVTVCPVGVF
                     ALGALPRAQRARLTLRGKLKGVAHHWQQAVLQAPQACEGCGLCVKACPERAITLVRTS
                     "
     gene            22238..22915
                     /locus_tag="AC16O9_10190"
     CDS             22238..22915
                     /locus_tag="AC16O9_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517026.1"
                     /translation="MTLKVFIGMDERQPLAYNVCRASIERHAGARLVIEPLRLKWLPI
                     RRRGLTDFTFSRYLCPYLCGYEGSALFLDADMIVRADVHELEALAPPDAPVSVVKGKL
                     RLEWPSMMFFRNALCRALTPAFIESGRPEAFEWTQRIGELPQEWNHCVGYDAPNPRPK
                     LIHYTQGIPCWPETEDCEHAEAWREEHRQANGTVSWHELMGRSVHARHVLRRLHAAGK
                     GKNSPEV"
     gene            complement(22912..23319)
                     /locus_tag="AC16O9_10195"
     CDS             complement(22912..23319)
                     /locus_tag="AC16O9_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6517027.1"
                     /translation="MAVRKVSYYSMKVPQRPGAGAQALAALKAAKVNLLAFTGFPASG
                     GAQVDFIPKDNAKFAAAARKAKMKLSARKTVFLSQGEDRVGALTQVLNKLAKAKINVV
                     SLQAVTAGKGRYGAMFWVKRKDLGKASRVLKAR"
     gene            23368..>23592
                     /locus_tag="AC16O9_10200"
     CDS             23368..>23592
                     /locus_tag="AC16O9_10200"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HIR6517028.1"
                     /translation="MGHRLSKITTRTGDAGDTGLGDGTRVAKDSPRIQALGDIDELNS
                     CLGLLLAEKPPAPIAKALLQVQHDLFDLGGE"
BASE COUNT         3196 a         8408 c         8399 g         3589 t
ORIGIN      
        1 ccgcgcgcaa ggaaggcatc atccccggcg cggcgaacct gcggctgccg cgcttcgtcg
       61 gcccgcgcgt cgcgcgccag gcgatcctcg ccgggcggcg gctcgattgc gcctcgcccg
      121 aggggcgcag gatctgcgac gagatcgtgc ccgagaccgg catggacccg gcgatcgaca
      181 gcctgatcgc gctgctcacc gattctggcg tggtgagcgc ggcgggcaac cggcgggcgc
      241 tgcgctcggg cgaggagccg ctcgagcagt tcaggagata catggccgtg tacgcgcgcg
      301 agcaggccgt gtgccacttc agcccggcgc tgatcgccaa tctggagaag cactgggacg
      361 cggcgaaccg gcgcggctga ttcaggcgag caggaactcc cgcacgaccg cgatctggtc
      421 cgcgtgcagc agcgtcggcg cgtggccgat gccgggcagc tccaccagct tcgcccgcgg
      481 cccgcgctcg gtcatcgcct gcgccgtctc gcgcgtcagc aggtccgact gcgcgccgcg
      541 gatcaccagg gtcgggcacg ccaccgcgtc ccacagctcc cacatctcca ggtccttgtc
      601 gggcatgtgc ttgcggtagg gctcggcgat cttcgggtcg tagtgcaggc gccagctgcc
      661 gtcggtgttc ttccgcagca ccacttcggt gagaaagcgc cactgcgcgt ccgtgtgcgg
      721 cccgaacggc gccgacaccg cgcgcatcac cttctccgcc gcctcgatgg aaggaaacga
      781 aggcgactga ccgacgtact ggccgatccg ctcgagcgcc gccttcgcca ccaccggccc
      841 ggcgtcgttc agcaccagct tcgccaccgg cgagcccttc tgcgcggcga gcgccatgcc
      901 gatgagtccc cccatcgagg tgcccaccca ctgcacgcgc tcgacgtcga ggcgcgccac
      961 gagcgtcacc atgtccgaaa cgtactgcgg gatctggtag agcatcgcgt cggcgagcca
     1021 ctcggagtcg ccgcgcccgg cgacgtccgg gcacaccacg cggtagcgcc cgcaaagcgc
     1081 gcgcgccagg ttgtcgaagt cgcgcgcgca gcgcgtgagg ccatgcacgc agatcacgac
     1141 gtcgggattg tgcgcctcgc cccactccag gtaggccatg cggtgcaggc ccgcgctcga
     1201 ggcgcactgc acgcgcttct cgcgcggcag caccgctagg ccgggccgcg gtccccggtc
     1261 cacttgtcca ccagcttgcc cgcttcgatc gcggcgccgc cgcgcgcgcc gtccttgtcc
     1321 cactcgactt ccacgcgcag ctcctcgaag gcgcgcgtcg cctcgacgaa cgccgggaac
     1381 gggatgccgc gggcgatctc gaagcgatac tcgagctcgc cggggcggtg gtgctcggtg
     1441 tagttctcgg cgtccatgtc gcgcaccatc aggaagcgca cgcgctcgcg gaactcctcg
     1501 aagcgcccgg ccgcgccggt gacgcgcacc accgccgtga acgccatcag ccgccgtact
     1561 tgaacgacag cgacaggcgc gcgcggaact gtgtcacctt gccgtcctcc accttcacgt
     1621 cgagcttggt gacttccgcg atgcgcaggt cgcgcagcga gctggcagcg gtcttcaccg
     1681 cgttcttcgc cgcttcttcc cacgaattgg ggctggtgcc gattacgtcg gtgacccgat
     1741 agaccgcgtt gtcgcctttg gccatgtgcg cctcctggaa gttggacggg agaacccccg
     1801 cggggccagt gtacgccgga ggcgcgggga cgtgccagta gcgcgcgcgg cgcgcgcgct
     1861 cgcgctcggc tgcgaggccc gcgggagaca gtcgcagcgt gagcgccccg tcgcggtcgg
     1921 tgcgcagcag ctgcgcgccc gctgcggcat agcgcgcccg cacttcggcc gcgggatggc
     1981 cgaagcggtt gcgatagccg gccgaagcca ctgcgaggcg cgggcgcact gcgtcgacga
     2041 acggcgccga ggacgagctg cggctgccat ggtgcggcac gaggagcgca tcggcgcgca
     2101 ccacgccgtc gcgcaagagc gcgagctcgg tgccgcgctc gatgtcgccg gtgaggagca
     2161 tcgcgctgcc gtccgtgctc accttgagca cgcagctttc gtcgttcctg cgccgcgccg
     2221 tgcgccccgg cgccggatgc agtatttcga accgcacgcc gtcccacgtc caggacgtgc
     2281 cgcgctggca gcgcttgggc cccggtgcga gcgcgagcag cgggtggcca tcggggagcg
     2341 agtgcagcag cgcgtccgcc tcgaagcctt cgagaatcga gcgcgcgccg cccacatggt
     2401 cgctgtcgtc gtgcgtgagc accagcgcgt cgagccgcgc cacgccgagc gcacgcagcg
     2461 cgggcgcgac gatgcgctcg ccactgtcgg tctcgccgta ggcggggcct gcatcgtaga
     2521 gcagcgcgtg gcgttcggtt cgtaccagca ccgcgagccc ctggcccacg tcgaacgtgg
     2581 tcacccaggc ttcgcccggc gccggcgccg gcggcggcag cgcgaccagg ggcagcatca
     2641 gcgcgaggcc ggcgccgcgc cacggaaagc cgcgcggcat gagaagccat gccacgccgg
     2701 cgagcgcggt gagcgtcgcc cagagcggcg gcacgtgctg ctgccagagc gctcccggga
     2761 gcgccgcgca ccactcgagg taggcgagga gccactgcac cagccactgc gccgcgtgca
     2821 gcagcgcgtc cacgggcagc acggcggcgg cgagcgcgag cggcgtgatc accgccgata
     2881 cgagaggaat ggcgagcgcg ttggcgagcg ggcccgccac cgagacctgg gcgaagagga
     2941 tcagcgccgc cggcgcgagg cccacggtga tggcccattg cacccgcagc cactgccgcc
     3001 agcgcggctc gggctgcgtc cagcccgtgc cgacatgaaa gatcagcgcc accgcgccga
     3061 acgagagcca gaagcccggc tgcagcacgg cccagggatc gagcgcgcac accagggcga
     3121 gcgccagcgc gagcgtgcgc gccggcgagg cgatgcgccc gctccacagc gccagcgcca
     3181 ccacgctcac catatagaag gtgcgctgcg ccggcacggc gaagccggca aggagcgtat
     3241 aggcgaacgc cgcggcgatg gcggttgcgg cccccgcctt tcgcgccggc aacgcgagcg
     3301 ccaggcgcgg cacgcgccgc cacagtgccg aggcgagcca cgcggcgagg cccgagacca
     3361 gcgtcacgtg cagccccgag atgctcatca ggtgcgtgac gccggtgcgg ctgaacaggc
     3421 gccattcctc gttggcaatg gcgcgctgct cgcccaccgc gagcgctgaa aggatgccag
     3481 cggcgggcgt cgcgccgaga acggcgtgaa agcgcacgcg cactgcggcg cgcgcctgcg
     3541 cgatgcggtc gacgaagctg ttgcgtgcgc cgaggcgccg agcgctttgc ggcgcgcgca
     3601 cgtagcccgt ggcgccgatg ccgcgctcgg tgagccaggc ttcgtagtcg aagccgtgcg
     3661 ggttgagcgg gccgtgcggg cgcttcaggc gcagggtgag cagccagcgc tcgcccggct
     3721 gcagctcggg cggcggctcg ccggccaggt cctcatacca gaagagcagg accctgccgg
     3781 gcagtcgcgc ggtcgacgac tcgggctcga actcgaagcg caggccgcgc tcggcggcgg
     3841 cgggcagccc ggaaacgacg cccgccacct cgacgtcctt gccttccagc tcgggcgcga
     3901 ggcggtcggc gaggcgcagg tgcgcggctc ccgccgccca caggaaacct gcggcgaatg
     3961 ccgcgggcac gacgagcgcg cgccgccacg cggcgagcgc cgcgcaggcc ggcagcgcgc
     4021 cgagccaagc gagcgcgggc agcgccgcct gctgctgcag gagcagcacc ccggcggtga
     4081 aggcgagcgc ggccgcggtc atctagaatc aacgcgccgg cgacgcgccg gatgaccgat
     4141 gccacgcaag ttcttccgca agtacctgcc gagcgccgac gaagtgcgcg gcaaccgcct
     4201 cgcggccctg ttcggcaagt tgctgcacga cccgaacttg tggcacctca accgcgattc
     4261 ggtggccggc gcggtggcga tcgggctgtt cgcgggcctc gtgccggggc cgctgcagat
     4321 gctggccgca ctcgccctcg ccattccctt ccggcgcaac ctgccggtgg cgctgctgac
     4381 gaccttctac accaacccgc tcaccatcgt gccgctctac gtgctcgcct acggctacgg
     4441 cagcctgctc ctcggacgcg acgtgaggcg gctgcccgtc gagccgttcg agatggactg
     4501 ggcgaacttc ttcgactcga tggcgcggct cgccgactgg acgctcgcgc tcggcaagcc
     4561 gctcgcgctc gggctggtgg cgctcgcgct gacgctcgcc gtgctgggat acctgctcgt
     4621 gcagctcggc tggcgcgcct acgtggtgct cgcctggcgc gcgcgccgcg agaagcggag
     4681 gcgccggtga cgcacgcgga gttcgtcgcc gcctatcgcg aagggcggct caaggtacag
     4741 gtggatcgcg aggctgcggc gcgccatgtc tcagcgcggc tcatgctgcc cgtcgtgctg
     4801 ctgcccttgc tcgggatcgc cgtggcgctg gcgctggtgg gccactacgt gatcggggga
     4861 ctggtgttcg cggccgcgct cggcctgcgc tttgcagtgc gccgcaccgc ccccggcttc
     4921 gtcctgcagc gcgccctcgc cgacgaaccc ttctacgccg aagccgtcgc ctcgcgacta
     4981 cttaaatagg gacagacctc atttttcttt cttgatacgc gactgcatat acgaaaagaa
     5041 aaatgaggtc tgtccctatt taagtgttcc gttgtcgagg tggaggacgc gctcggcgcg
     5101 cccggcgagc gccgtgtcgt gggtgacgat gacgaaggcg gtgccgcgcg cggcggagag
     5161 tttcagcatc gcctcgaaca cctcgtccgc ggtgttcgag tcgaggttgc cggtgggctc
     5221 gtcggcgagc acgcacgccg gctcggtcac cagcgcgcgc gcgaaggcgg cgcgctggcg
     5281 ctcgccgccc gagagctcgc ccggcaggtg ctcgacgcgc gccgcgaggc cgatctcggc
     5341 gagcgccgcc tgcgcgcgct cgagcgccgc gccgcgcgcc atgcgccgga tgaagagcgg
     5401 catggcgacg ttctcgagcg ccgtgaactc gggcagcagg tggtggaact ggtagacgaa
     5461 gccgagcgtt tcgttgcgca cgcgcccgcg ctcggcctcg tccatctcgg caaacggcct
     5521 gccccgcact tgcaccgtgc ccgcgctcgg cacgtcgagc ccgccgagca ggtgcagcag
     5581 cgtcgacttg cccgagcccg acttgcccat gatcgccacg cgctcgcgcg cgcgcaccgc
     5641 gaaatccacg ccgcgcagca cctcgaccgg cgccgggccc tggtaggtct tcttcaggcc
     5701 ggcacaggcg agcaccgcgt cactcatagc gcagcgcctc cgccggattg acgcgcgagg
     5761 cgcgccagct cggatacagc gtggcgacaa gcgacagcac gagcgacacc gcgcacacga
     5821 tcgccacgtc cataaactcg accttcgagg gcaggtccgg gatctggtag acgtccttcg
     5881 acaggaactt gaagccgagc accgtctcga gcgcgggaac gacgacgtcg acattgaagg
     5941 cgagcgcgag gccgccagcg aagccgagca gcgtgccgat gacgccaatc acgctgccct
     6001 gcacgatgaa gatctgcatc acggagcgcg gcgccgcgcc gagggtgcgc aggatggcga
     6061 tgtcggcctg cttgtcggtc accagcatca ccagcgtcga cacgacgttg aaggcggcca
     6121 cggtgatgat cagcaccatg atgatgaaga tcatgcgctt ttcgatctcg atggcgcgga
     6181 acagattcgg gttggcccgc gtccagtcgg aggcttccag gccgcgctcg gaacgcagcg
     6241 tgatctcgcg cgcgaccccg cgtgcctcga gggggtcgtc gagcttcaag cgcacgcccg
     6301 acaccgcgtc gccgatctgg tacaggcgct gcgcgtcggc gaggtggatg agggcgagcg
     6361 cggcgtcggc ttccgcgtag ccgacgtcga agatgccgac caccgtgaac tgcttgagcc
     6421 gcgggatcac gcccgccggg gtgacgctgc cgcgcgggat gatgagcacc agcttgtcgt
     6481 cgatgtaggt gcgcagcgcg cgcgccaggt cgccgccgat cacgacgccg aactcgtccg
     6541 cccgcagcgc ctcgaggctg ccggcgcgca tgtgctgcgc gatgtcggtg acccgcgact
     6601 ccggccccgg caggatgccg cgtaccagca cgccgcgcgc ggcgccgctc accgtgacca
     6661 tggcctggcc gtgcacgtac ggcgcgctcg cctgcacgcg cggcagcgcc gcgacctcct
     6721 gggccacgcc ctcccagtcg gcgagcccgc cgcgcgggtc gtacacctgc acgtgcgagg
     6781 ccacgccgag gatgcgcgtg cggatctcct cctggaagcc gttcatcacc gacagcacca
     6841 cgatcagcgc cgccacgccg agcccgatcc cggccatcga gatgagcgag atgaagctga
     6901 tgaagtggtt gcgccgcttg gcgcgggtgt agcgcaggcc gacgaggagt tcgtaccgca
     6961 cggggccacg agaatactac aatcgctgcg atgaaccccg acgatgccgc ggcgctggcg
     7021 cggctgctgc gcaccgggcg cgtggcgcac ctcggcacgc tgcgccaggg cgcgccgctt
     7081 gcgtcgatga cgctgtatct cgcggcgccc gacttcagcg cctgctacgt gcacgtgagc
     7141 cgcctcgcct ggcacacgca ggacatgcag gccgatccgc gcgtcgcgct gtcggtgtcc
     7201 gaaaccgacg acggccgcga ggacccgttc acgcttgcac gcgtcacgat ccgcggcgtg
     7261 gccgaggcgc tcggcgccga gcctggactg acgcaggcgt ggctggcgcg cttcccggcc
     7321 caggccgtga acttcgagct cggcgacttt tccttctggc gcatcgtgcc gcacgacgcg
     7381 cgcttcgtcg ccgggttcgg gcgcatccac aacctctcgg ccgccgagct cgccgcggcg
     7441 gcgcgcgtgt gagcgcgctc ctcgccctcg ccctcgcggc ggcgccctgc gcggtcgagc
     7501 ccgagtggcg caacgagggc gtggtgcagc ttctgcccgc ctgcgcgccg gggtacacgc
     7561 cgacgctgga aggcgtgcgc acggcgctgc gccaggcggg ccggcgcggg gagctcgtcg
     7621 tcggcttcgg gcgcgtggcg cactacccgt ggctgtcgtc gttcctcgcg cgccaggcct
     7681 cagcgtcgcg ccactggccc gaggagcgcg ccgcgcccga gggctacgtc acgcgggccc
     7741 tcatgggcat gccggagttc acggcgctct tcgatccggc atggcgcatc gcgagcatcg
     7801 tggtgcagga cgtgcgcacg aagccggccg ccgagctcga cctgccgcag ggccatccgc
     7861 tgccgccgga cttcctgctg ccgtacgatg cggagctcgt cgtgacgctg gtgcgctgac
     7921 atggtgacgg tctatggcat ggcggactcg ggcaactgct acaaggtgaa gctcgcgctc
     7981 gagcagctga agctccccta ccagtggatc gaggtgagca ccaccagggg cgaaacgcgc
     8041 acccgggagt tcctcgcgcg caacccgaac ggccaggtgc cgacgctgca gctcgaggac
     8101 ggcgcatacc tgcccgagtc gggcgcgatc ctttggtacc tcgccgaggg caccccgctc
     8161 ctgcccgccg agcgcctcgc gcgcgcgcgc gtgctgcagt ggatgttctt cgagcagtac
     8221 agccacgagc cgtgcatcgc ggtggcgcgc tcgatcctgc gctaccagcc gcccgactcg
     8281 ccgcgccgtg ccgagctgcc gcggctgcag gagcgcgggc acaaggcgct cggcgtgatg
     8341 gcgcagcagc tggagaagtc ggatttcttc gccgggtcga agtactcgat cgccgacatc
     8401 gcgctctacg cctacacgca ctgcgcggct gatggcggct tcgagctcaa gcgctacccg
     8461 gcggtgctcg cctggctgat gcgcgtgaaa tcgcagccgg ggcacgtgcc gctcggctag
     8521 cttttgcggt agagtcgcag tactggatgg atatccatat ctgggcttgg ttcgctcccg
     8581 tgctggccga cacccccctc gccatcgtcg acctggaaac caccggcgcg cacccgctgc
     8641 acgaccgcgt caccgaagtg gcggtgatcg aggtcgagca cggcgaggtg cgggacgaat
     8701 ggtctacgct ggtcaacccc gagtgcgcga tccccggggc gatccaggcg ctcaccggca
     8761 tcaccaacga catggtggcg ggcgcgccga ccttcgctcg cctggcgcag gagctgcacg
     8821 agcgcctcga agggcgcgtg ctcgtcgcgc acaacgcgcg cttcgactac ggctttctgc
     8881 ggcaggaatt ccagcgcgtg ggtctcgcgt ttcgcgcgaa gacgctgtgc acggtgaagc
     8941 tctcgcggcg actgtacccg gagcacgcgc gccacaacct cgacagcctc atcgcgcgcc
     9001 ataacctgga ttgccgcgcg cgccaccgcg cgctcggcga cgccgaggcc gtgtggcagt
     9061 tcctgcgcct cgccgccgag gagcgcggcg aggaggtgct ggccgtggcg gcgcgccagg
     9121 tggcgaagca accctcgctt cccgcgcacc tcgaccgcgc catggtggac gcgatccccg
     9181 aggcgccggg cgtgtacctg ctctacggcg aatcgggcgc gccgctctac gtgggcaaga
     9241 gcatcgacat gcgcgcgcgc gtgctctcgc acttcgccga cgacctgcgc tcgccgcgcg
     9301 agatgcagct cgcgcgcgag gtgcgccgcg tcgagtggca gcgcaccgcg ggcgagctcg
     9361 gtgcgctgct gcgcgaggcg cagctggtga aggagctgct gcccgtcttc aaccggcagc
     9421 tgcggcgccc ggcggatctc tgcggcttcg ccttcgacgc gaagcgcctg cgcctcgtgc
     9481 acgacttcga cgccgagggc atcgcgcacg tgcacggcct gttccgcagc cgccgcgcgg
     9541 cgctcgccgc gttgcgcggc ctcgccgacg cgcacggcct ctgcctgcag tcgctcgggt
     9601 tcgagaccgc gcgcaaaggc gcgtgcttcc ggcaccagat ccggcgctgc gcgggcctgt
     9661 gcgccgggca ggaatcggtg cacgcgcacc acgcgcgcct cgccactgcc cttgcgggcc
     9721 tgaagaacgc cgcctggccc tggcgcgggc cggtgggcgt ggtggaggaa gacgcggcgc
     9781 gcgaggcgac cgaggtgcac gtggtgcacc agtggtgcta cgtcggcacg gcgcgcgccg
     9841 aggacgaggt gccggcgctg ctcgagtcgg cgcgtcgccc gcgcttcgat ctcgaccagt
     9901 acaagatcct gcagcgccac ctgcgggcgc gccgcgcgcg cgtcgtggag ctcgcgtgca
     9961 cgtcgagctg atggtgccgg gggtgttcgc cgcgccgccg gggccgcgcc gcccggcgct
    10021 ggagctgctg ctcgcgcgcg cgcgcgtggt gcacgccgac cccgagagcg ccgagacctg
    10081 gctcggccgc ggcttcggcc aggaacagtt gcccgccggc gcgctcaccg cgggcgcgcc
    10141 cggcttctgg ctgcgcgccg atcccgtgca cctgcgcgtg ctgcgcgacc gcgtcgtcat
    10201 cgtgccgctc gccgggctcg cgcccgagac cgcggcggcg ctcgtcgcga cgctcaaccg
    10261 ccacttcgcc ggccggcacg agttccgcgc gccgcacccg gatcgctggg taatgtcgaa
    10321 cgcgccggag gcgcttcggt cgagcccgcc cgcgccgctt cgagcgccgc cggcgcgcgc
    10381 gctggcgggc gcggacttcg gcgcgcacct gccggccgcg ccctggcccg cgctgctgaa
    10441 cgagatccag atggcgctgc acgagcaccc ggcgaacgaa ggacgcgagc tggaggtgaa
    10501 cggcgtatgg ctgtggggcg cgggcgagct gcccgcgacg gccacggcgc cctggcgctc
    10561 ggtgagcgcc gaggacccgc tcgcgctcgg cctggcgcgc atcgcgcgcc tcgcgcaccg
    10621 cacgccgccg gcctcggcca ccgagtggct cgccgagctg cccgaggaag ggcgccacct
    10681 cgccgcgttc gacgcgctgc acgagtcgct ggaggccgac tggttcgcgc cgctcgtggc
    10741 cgcgctgcgc gcggggcgca tcggcatgct gacgctgcac gtgcccgacg ccggctgcgc
    10801 cttcgaggcg gtgcgcgcgg acctgcgccg cttctggcgc cgcccgcgcc cgctgcaggc
    10861 ccatgcgcct cgttgagcgc ccgtacgacg aggtcgaccg gcgcgcgttg atcaacgccg
    10921 ggctgcaccc ggtgctcgcg cgcgtgtacg ccgcgcgccg catccgctcg gccgccgagc
    10981 tcgaggacga cgtgaagcgg ctgctgccgc ccgcctcgct cgcgcgctgc ggggaagccg
    11041 cggtgctgct cgccgacgcc atcgccgcgc agaagaagct cctcatcatc gccgactacg
    11101 acgccgacgg cgccaccgcc tgcgcggtgg gcgtgcgcgc gctgcgtgcc atgggcgcca
    11161 gcgtcgacta cctggtgccg aaccgcttcg agctcggcta cggcctgacg cccgagctcg
    11221 tcgacatcgc cgccgcgcgc aagcccgacc tgctcatcac ggtggacaac ggcatcgcga
    11281 gcgtcgaggg cgtggcgcgc gcgagcgcgc tcggcatcgc cacgctcgtc accgaccacc
    11341 acctgcccgg cgccgagctg ccgcgtgccg cgtgcatcgt caacccgacc cagcccggct
    11401 gcggcttcgc ctccaaggcg atcgccggcg tgggcgtcat gttctacgtc atgctcgcgc
    11461 tgcgcgccga gctgaggacg cgcggcgcgt tcaaggacgg caaggagccc aacctcgccg
    11521 cgctcaccga cttggtcgcg ctcggcacgg tggccgacgt ggtgccgctg gatgccaaca
    11581 accggatcct ggtcgccgag ggcctgaagc gcatccgcgc cgggcgcgca cagcccgggc
    11641 tcggcgagct gctgcgcgcc gcggggcgcg gcgccgcgca ggccggcggt ttcgacctcg
    11701 gcttcatcct cggcccgcgc ctgaatgctg ccggccggct cgccgacatg agcctcggca
    11761 tcgagtgcct ggtcaccgac gacgcggcgc gcgccgccaa ctgcgcgcag gagctcgatc
    11821 gcctcaaccg cgagcggcgc aggatcgaag ccgagatgct cgagcaggcg cgcgcggccg
    11881 ccgatgccat cgccgaggcg ccgggcgcgg cggtcgcgct ctacgatccc gcgtggcatc
    11941 ccggcgtggt cggcatcctc gcctcgcgcc tccgcgagcg cctgcaccgg ccggtgatct
    12001 gctttgcgcg cgcgaacgaa acggcgcagt cgggtcaact tcgcggctcg ggccgatcga
    12061 ttccgggctt tcacctgcgc gactgcctcg acctcgtcgc caagcgcgac cccggcctca
    12121 tcgtgcgctt cggcggccac gcgcaggccg cgggcctcac gctgcgcgaa acggacctgt
    12181 cgcggtttgg cgacgctttt gcgcgcgccg ccgaggaaac gatgccgcgc gaggcgctca
    12241 cgcgcacggt ggagaccgac ggcgcgctcg agccgcagca attttcgctc ggattggcgc
    12301 aattgctcga agcgcgcgta tggggtccgg gctttccgcc gccactcttc tgcgatacat
    12361 tcgcgctcga aagccagcgc gtggtcggcg agaagcattc gaagctgcaa ctcgtgaagg
    12421 acggccgccg cctgcaagcc atgcgcttca acgccctcga cccgctgccg acccgcttcc
    12481 gcgccgccta ccggctgagc gtgaacgagt acaacggcgt gcagagcgtg caggtgagcc
    12541 tcgagcacgt ggaaggcgca tgaggctgct cgcgctcctc gcgctcgcgc cgctgctggt
    12601 ccttgcgcag gacaagccgg tcgacgccgg tccctacgtc ccctcgccgc agagcgtggt
    12661 ttccgacatg ctgaagctcg ccgaggtcgg cccggaggac ttcgtcatcg acctcggctc
    12721 gggcgacggc cgcatcgtgc gcaccgcggc gctgatcttt ggcgcgcgcg gcttcggcgt
    12781 cgagatccgc gacgagctgg tgcgccagtc gaacgccgac gcggcgcgcg aaggcatcgc
    12841 cgaccgcgtg aagttcgtca atgcggatct cttcaagacc gacgtctcgc aggccacggt
    12901 gctcaccatg tacctgctgc cggacacggt gaacctgctg cgcgagaagc tcctgcggga
    12961 gctgaagccc ggcacgcggg tgctgtcgca cgactacccg ctcgcgggct ggatcccgga
    13021 gaagtacgtg cagatggacc tcgaggacaa ggtgaagatc agcggggtca ccaccaccct
    13081 gatctatctg tacgtggtgc cggcgcaggt cggcggccgc tggcaggcgc gcggtcccgg
    13141 cggcgccctg acgctcgacc tgaagcagca ggtgacgcgc gtgtcgggca gcgcgcggca
    13201 cgccggccgg gacagcgcgc tccaggacgt gaagctgcgc ggcaccgagc tctcgttcgg
    13261 gctcggcggc aagacctggc gcgggcgcgt ggacggggcg agcatcgcgg gcgagatcga
    13321 aggcggcggc gcctggagcg cccggcgtgg caagtgatgg aacgcaagcg cgtcaattcg
    13381 tcgaaagtgc gcgcggtcgg ctacgacgag cgcagccaga cgctggaggt cgagctttcg
    13441 aacggccagg tgtggcagta ctcgaaggtc taccccgagg tgtaccgccg cttcatggcg
    13501 gcgcccaatc ccgccacctt ctacgaagac aagattgccg aggactatac gggccggcgc
    13561 gtttagcgcg ctcctcgccg ccgcaacggc cgcggcggcc atcgagcccc cgtacgaagc
    13621 cgtgcagcgc ggcgccgact gcaacctgcg gcccgacggc agcctcggct gccgctaccg
    13681 cgtcggcaaa gacctggagt tcgagctgca ccgcatcggc gagcgcggcg tcgggctgcg
    13741 cctcacgcgc tcgagcgagg cgggcgacta ccgcgcggac cgcgagatga tgtcgcgctg
    13801 cgtgttcgtg cgccacggcg agcgcgggcg cgaggcgggc ggtggcgagt tcgactacgc
    13861 gttcgtctcc ggccgcaacg gcttcgtcta ccgcagcctg cgcgagtgca ggctggcgcg
    13921 atgagcgcgc agctcgtcgc ggtgctggtg ctcgctgcgg cgtggctcgc catcggcgcc
    13981 gcgggtctcg cggcgctgca ccgcctgcac ctcgtcgcgc gctggctgtt tcccgccggc
    14041 ggcgcagtgg cgctgctcat cgctgcggcg gcgctgccgg cgctgtgggg cgcgccgcag
    14101 gcggtagtgc tgccgatcgg cctgccgggt gcgccgttcc acttccgcct cgacggccta
    14161 tcggcgttct tcctgatcgt gctgggcggc gcggcggccg gcgtgtcgct cttttccgcg
    14221 ggctacttcc gcagccgcga aggcaccgcg ccgggtctgc tgtgcctgca gtaccacgtg
    14281 ttcctcgccg ccatggcgct ggtgatcctg gccgacgacg cctacgcctt catggtgatg
    14341 tgggagacga tggcgctcgc ctcgttcttc ctcgtcatca ccaaccaccg cctgcccgag
    14401 atccgtcgcg cggcgtactt gtacctgctc atcgcgcaca tcggcgcgct cgcgatcctg
    14461 ctttgcttcg gcgtgctgca ggcaaacacc ggcgacacca gcttcgccgc catgcgcgcg
    14521 cagacgccca cgccgttctg ggcctcggtc gcgttcctgc tcgcgctcgc cggcttcggc
    14581 gccaaggcag ggctcgtgcc gctgcacgta tggctgcccg aggcgcatcc ggcggcgccc
    14641 tcgcccgtat cggcgctgat gagcggcgtg atgctgaaga ccgccgtgta cgggttcctg
    14701 cgcgtcgcct tcgacctgct gccggcgcag ctctggtggt ggggggtgct cgcgctcgcg
    14761 ctgggcctgg cgagcgccgt gttcggcgtg gcattcgccg cggtgcagtc ggacatgaag
    14821 cgcctgctcg cctggtcctc ggtcgagaac ctgggcctta tcttcgccgg ggtcggcctc
    14881 gcgctcctgt tcgccgcgta ccggatgccg gcggcggcgg cgctcgcgct caccgcggcg
    14941 ctctaccacg tcgcgagcca cgcgttcttc aaatcgctgc tgttcgtcgc caccggcgcc
    15001 gtgctgcacg cgaccagcga gcgcagcctc gggcgcctgg gcgggctgct gcgcgtgatg
    15061 ccgtgggtgg gctgggcgtg cctggccggc gtgctggcga gcgccgggct gccgccgctc
    15121 ggcggcttcg tctccgagtg gctgctgctg cagagctttc tgttcacgcc ggggctgccc
    15181 aaccccttcc tcaacatgct ggtgccggcg gtggcggcag tgatcgcgct tgtcgccgcg
    15241 ctcgcgggct acgtgatggt caagttctac ggcgtgatct tcctcggccg ccaccgcgag
    15301 gacaagctcg ccgcggcgcg cgacgcgggc tcctgggagc ggcttggcat gggctggctg
    15361 gtggcgggct gcgtggcgct cggcctgttc cccgcgctcg gcatcgggct cctcgcgccc
    15421 gtcaccgagc tcctcgtggg cgcgcgcctg ccggaggcgg gcggctggct gctggtaccg
    15481 gtgtcggcgg cgcgcgcgag ctacgggccg ctcatcttct tcgccggcgt ggcggcgagc
    15541 gtcggcctcg ccttcgtcct ggtgcggcgc ctgtaccacg ggcggctgcg gcgcgcggcg
    15601 ccctgggact gcggcttccc ctggcagacg gcgcgcatgc aggacaccgc cgagggcttc
    15661 ggccagccgg tgcgccagat cttcgcgccg ctgtaccgca tgcgccgccg cttgccgagc
    15721 gcgttcgacg ccgagccgcg ctacgcggtc gaggtaggcg accatttctg gcactggctg
    15781 tacctgccgc tcgggcgcct ggtcgagtcc accgcgcgcg tcatcggcac gctgcagcgc
    15841 ggccgcatcg cgctgtacct ggtgtacagc tttgccaccg tgatcgcgct gctcgtctgg
    15901 gtgacgcggt gagcgcgctc ggcctcgcct cgcaggcgct cgagatcgtc gccgcgctcg
    15961 cgctggcgcc gctctttgcg ggctgggtcg cgcagtgccg cgcctggctg cagagccgca
    16021 ctgcgcccgg gctgctgcag ccctaccgcg tgctgcataa gctctttaac aaggactcgg
    16081 tgctggcggc gcatgcgagc ccgctgttcc ggatcgcgcc gtacgtgatc ttcggcgcca
    16141 tgacgctcgc ctgcggcatc gtgccgacgc tctcgaccga gctgccgctc tcgcccgcag
    16201 ccgacgccat cgcgctggtg ggcctgttcg cgctcgcccg cgtgttcgcc gcgctcgggg
    16261 cgatggatgt cggcaccgcc ttcggctcgc tcggcgcgcg ccgcgagatg ctggtcgggt
    16321 tcctcgccga gccggcgctg ctgatggtgt tcttcaccgc cgcgctgatc tcggactcga
    16381 cctcgctcac caccatcgtg cgcacgctct cggcgcgcga gatcggcatc cagccgagcc
    16441 tcgcgctcgc cgccgtggcg ctcgtgatgg tggcgctcgc cgagaacgcg cgcgtgccgg
    16501 tggacaaccc ggccacgcac ctcgagctca ccatggtgca cgaggcgctg atcctcgagt
    16561 actcggggcg ccacctggcg ctcctcgagt gggccgcggc gctcaagctc ttcgcctacg
    16621 cgtgcatcgg catggcgctg ttcgcgccct ggggcctggc gcacgcgcag gcgccgctcg
    16681 cgctcgtcac cgcgctgccg gtgctggtgg cgaagctcgc tgcggccggg ttgctgctcg
    16741 ccctgctcga gaccgtgagc gcaaagatgc gcatcttccg cgtgcccgag ttcctcggca
    16801 tggcgttcat gctggcggtg atcgccatgc tggtgaagat cctgctcggc ggcagcgcgc
    16861 catgaccggg ctgatgccgc agctggtgaa cctgttcgcg gcgctgctgc tgctccttgc
    16921 cttcgccatg ctggcgcagc ggcgcatcct gtcgctgatt catctgtaca cgctgcaggg
    16981 cgtggtgctg gtcgcggcgg ccgcggtgat gggctacgcc acgcgccagc cgcacctgta
    17041 cgtctcggcc gcgctgacgc tcgcgctcaa ggtgctgctg atcccgtggc tgctgcatcg
    17101 cgtgatcgac cgcctcaacg tgcgctggga cgtcgagatg ctgatcaacg tgcccacgac
    17161 gatgctcgcc ggcatcatgc tcgtcatctt cgccttctac ctcgcggcgc cgatctccgc
    17221 gctcgctccg tcgatcgcgc gcggcacgct cggcatcgcg ctcgcctgcg tattgctctc
    17281 gttcatgatg atgatcacgc gctccaaggc ggtgccgcag gtgatcggct tcctcgccat
    17341 ggagaacgcg ctctttttcg ccgccaccgc cgcgacctac ggcatgccga tggtggtcga
    17401 gctcggcatc gcgctcgacg tgctggtcgg catcctgatc ctcggcgtgt tcctgttcca
    17461 gatccgggag cagttcgaca gcctcgacat ccgccacctg gagaagctca aggatgacta
    17521 gcgccgtcgc gctcggcttt ctcctcggcc tgccgctcgc cggcgcgctc gcgctcgcgc
    17581 tcgccggcca ccggcgccgg gcgcccgagg cgaacatcgc cttcagcgcc ggcaccttgc
    17641 tcgccgcggg cgtgctcacc gcgcgcgtcg ccgcggccgg cccggtgttc gtgctcgggc
    17701 gcgcgttcta catcgacgcg ctgaacgttt tcctggtcgc gctcaccgcc ttcgtcgcgc
    17761 tcaccacggc gatcttctcg cgcccctaca tgcgcgtcga gcaggagcac gggcgcatga
    17821 atccgcgccg gctgcggctc taccacagca tgtaccagct gttcagcttc accatgctcc
    17881 ttgcgctcac caccaacaac atgggcctcc tgtgggtcgc gctggaggcg gcgatgctcg
    17941 ccacggtgct cctggtgagc gtgtaccgta gcgccgcgag cctcgaggcg gcgtggaagt
    18001 acttcatcct gtgcggcgtc ggcattgcgc aggcgctctt cggcacggta ctgctctata
    18061 tggcggccga gcgcgcgggc gcgggcgagc acggcacgct cctctggacg cacctcgacg
    18121 ccgtcaaggc gagcctcgac ccgcgcatca tggcgctcgc cttcgccttc ctgttcgtcg
    18181 gctacggcac caaggtcggc ctcgtgccgg tgcacaactg gctgcccgac gcgcatgccg
    18241 aaggcccgac gccggtgtcg gcggtgctct cggggctgct gctgaacgtc gcgctctacg
    18301 ccgtgctccg ctgcaaggtg ctcgccgacg gcgcgctcgg cccggggctc gccgggcaga
    18361 tgatgatggc cttcggcctc atctcggtgg tggtggcggc gttcttcctc tcgcgccagc
    18421 gcgacgtgaa gcgcatgttc gcctactcct cgatcgagca catggggctc atcaccttcg
    18481 ccttcggcgt cggcgggccg atcgccacct tcgcgggcct gctgcacatg acggtgcact
    18541 cgctcgtcaa gtcggcgatc ttctttgcca tcgggcacgc cgcgcagcac gccggcaccc
    18601 aggtgatgag cgagatccgg gggctggtaa aggcgaaccc gagcgtgggc tggggactga
    18661 tgctcggctc gctcgccatc ctcggcatgc cgcccttcgg cgtgttcgcg agcgagttcc
    18721 tgatcctcac cacgacgatg cgcgagcagc cctgggcggc gcctttcctg ctcgcggccc
    18781 tgggcgtcgc gttcgcctcg gtgctgcagc gcgtgcagcc gatggtgttc ggcgaaacgg
    18841 agctgaagcc gctgccgcag ccgccggcgc tgatccccgt gttcgtgcac atcgggctcg
    18901 tgctcgtgct cgggctgtac atcccgccgt ccctcgaggg ctggtaccgg gaggccgcgc
    18961 gcatggtggc cggatgaggc gcgaggtcct gaagcgcgag cactgggtga gcgcggcgcg
    19021 cgcggtggtg gagcgcggcg ggcggctggt ggcactgtgg ggctcggagc ggcgcgaggc
    19081 gtacttcgtc tgcgcggcct atgccacgcc cgaggggctg gtgtggtcgg agctgccgct
    19141 ggatggcggc gcgacgtatc cggacctgtc gccggtgttc tactttgccg ggcgcatgca
    19201 gcgcgcgctg gcggacctgc tcgggctgac cgcggaggaa gcgctcgatc ggcggccgtg
    19261 gctcaaccac ggcgcgtggc cgtacgggta tcaccccttg ctcagcaatt cgaccggcaa
    19321 ggaggtgttc gacgactgcg acgtcaccga ttaccccttc gtgcacgtgc aaggcgaagg
    19381 cgtgcacgag atcccggtgg ggccggtgca cgcggggatc atcgagccgg ggcatttccg
    19441 cttttccatc gtcggcgaga aggtgctgcg gctggagaaa cggcttggct acacccataa
    19501 aggaatcgag aagcgcttta cccagctgcc gccggaaggc gcgcatctcc tggccgggcg
    19561 cgtgtcgggg gattcgaccg tggcttgcgc ctgggcgtat tgcatggcgc tggagaacgc
    19621 agcggacgcg gaggtgccgg agcgcgcgtc ctggctacgc gcgctgctgc tggagcgcga
    19681 gcgcgtcgcg aaccacctgg gggatctggg cgcgctggga aacgacgccg ggtttgcttt
    19741 cgccttgacc cagttctcgc ggctgcgcga gcagtggcag cgcgcctcgc gcgaagtgtt
    19801 cgggcaccgc ttcatgatgg accgcgtcgt gccgggcggc gtggccgagg acgtgaacgg
    19861 cgcggcgctc gagctgatgc gcgaggactg cgccgcgttt gccgcggagg tgaaggcgct
    19921 gcgcgtgatc ttcgatgagc acgccgggct gcaggaccgc ttcattgccg ccgggcgcgt
    19981 gcggccggag ctcgccaacc gtctgggcgt caccgggctg gtggcgcgcg caagcgggca
    20041 ggcgatcgat ttgcgcaacg accatccctg ccgtccctat gacgagctcg gggtgcagat
    20101 ggcgacgcat cgcaatggcg acgtcgccgc gcgcgtcatc gtgcgcttcg aggagctgct
    20161 cgagtcgctc cggctgatcg tggagatctg caaccgcctg cccgagggcg cggtgcgcgg
    20221 cgaggtggag attccggtcg gcgatgccta cggcgccggg tgggtggaag ggtggcgcgg
    20281 cgaggtgttc tgcgggctgg agctggagaa cgggcggatc cggcgctgcc atctgcacga
    20341 cccgtcctgg cagaactggc cggcgctcga gcacgccatc atcgacaaca tcgttccgga
    20401 ctttccgctc atcaacaagt cgttcaacct gtcgtattcg ggccacgacc tctgatgctg
    20461 aagaccgtca tcaagatcgt gcgcaccggg atcctgaccg agcccgcgcc gacggtgcat
    20521 tcgctgcccg aggtgtcgcg gattcaccat gagctgctgc acatcctggg tcgggcgctg
    20581 tgcatccggc aggtggacgc ggggtcctgc aacggctgcg agctcgagat ccatgcgttg
    20641 aacaacccct actacaacct tgagggttac ggaatcaagt tcgtcgccag cccgcgccat
    20701 gcggacttgc tgatggtgac cgggccggtg tcgcggaaca tggagctggc tttgaagcgg
    20761 acgtacgagg cgattccgga tccgaagctg gttctggcgg tcggggattg cgggtgcgac
    20821 ggcgggatat tcggggagag ctacgccagc tgcgggcggg tttcgaatgt cgtgccggtg
    20881 gatttgacgg tggcgggatg tccgccgccg ccgctggaga tcctgcgggc ggtgctgggg
    20941 ttggcgagga ggttgaggta gggccggcga gctgtcggca aacgtgtggg ggttctatgt
    21001 cgccgcctga cagcccgttg gtggccgaca cccttcgcta cgcactttgc gcggcgcggc
    21061 cgagcgtaaa cgcagagcct gcgaggatgg gtcgcacggt gtcgagaacg ccagcagccc
    21121 tgacgatttg tgcctgcctc gggatgatgg cgccgctgtc cgccccggca tccgaacgct
    21181 ggttcctgat gtcgcgtcat ggagattgcg cgccggtggc gacgctcaag cgcaaggttc
    21241 cggaccttgg cgagatcaac gacccggtcg cgttcgcggc gttcatgcga cgcaagggcg
    21301 tcgccgtgac ctcgacccgg gtccccgtgc cgaaaggcag ggccgaggag gtgaaggtgc
    21361 ccgagaggca cttgttcctg atgttcgtaa cgtcgcagat gtgccgcggc gctgcgcggt
    21421 gagcgctcat tcgagcgggc ggcctgaagt cgtccggttg ctggaggtcg ccgcgcccgc
    21481 ggcgccgtct gctcggcgcg cgaagggccc ttcgcgccgc tggacgccgg gttcgccacg
    21541 cgcgcgcatc gccgtcgagg acggtccggg gggccgcggc acgcgggtgg tggtgcgttt
    21601 cgaagccgtc taggccgggc gcgggatggt atggttgcca gtgcaggtgc cggcatccca
    21661 cgcggcattg aaggaggcca ccatgacgga cccggcagcc gagaagaccc cgctggagcg
    21721 atcgcgggac atcatcaagc agttaaagga gatgctgcac tactcggaag gcaacatcga
    21781 gaagctgacc gggatctggc tcctgctcga cggcgagctg aagcaaaaga gagtcgccaa
    21841 gggcctggaa gcgctcctca cgcaccagaa caagttccac gacgcgctcg ccgcgctaat
    21901 caaggactac gagtcggaat gcgacaagct ggagaagaaa tagcgctcat ccaatgccgg
    21961 cacgaccccg gcgtgttcgt accggtgatc gaccgcaatc gctgcgaagg caaggcaaga
    22021 tgcgtgacgg tctgcccggt gggcgtgttc gcgctcggcg cgctgccgcg cgcgcagcgc
    22081 gcacgcctca cgctcagggg aaagctcaag ggcgtggcgc accactggca gcaggcggtg
    22141 ctccaggcgc cgcaggcctg cgaaggctgc ggcctgtgcg tgaaggcctg cccggaaagg
    22201 gcgatcacgc tcgtgcgcac gtcatagggg agccgcgatg acgctgaagg tcttcatcgg
    22261 catggacgag cgccagccgc tcgcctacaa cgtgtgccgc gcctcgatcg agcgccacgc
    22321 cggcgcgcgc ctcgtgatcg agccgctgcg cctgaagtgg ctgccgatca ggcggcgcgg
    22381 cctcaccgac ttcaccttca gccgctacct ctgcccctac ctctgcggct acgaaggcag
    22441 cgcgctgttc ctggacgccg acatgatcgt gcgcgccgac gtgcacgagc tggaagcgct
    22501 tgcgccgccg gacgccccgg tctcggtggt gaaaggcaag ctgcgcctcg agtggccgag
    22561 catgatgttc ttcaggaatg ccctgtgccg cgcgttgacg cccgcgttca tcgagagcgg
    22621 caggcccgag gccttcgagt ggacgcagcg aatcggcgag ctgccgcagg aatggaacca
    22681 ctgcgtgggc tacgacgcac ccaacccgcg cccgaagctg atccactaca cgcaggggat
    22741 cccttgctgg cccgagaccg aggactgcga gcacgccgag gcctggcgcg aggagcatcg
    22801 ccaggccaac ggcaccgtct cctggcacga gctgatgggc cgctcggtgc acgcgcggca
    22861 cgtcctccgg cggctgcacg ccgccggcaa gggcaagaac agccccgagg tctaacgggc
    22921 cttcagcacg cgcgaggcct tgcccaggtc cttgcgcttc acccagaaca tggcgccgta
    22981 gcggcccttg cccgcggtca ccgcctgcag cgagacgacg ttgatcttcg ccttggcgag
    23041 cttgttcagg acctgcgtca gcgccccgac gcgatcctcg ccctgggaaa ggaacacggt
    23101 cttgcgcgcc gagagcttca tcttcgcctt gcgcgcggcc gccgcgaact tcgcgttgtc
    23161 cttgggaatg aaatccacct gcgcgccgcc gctcgcagga aagccggtga acgcgagcag
    23221 gttgactttc gctgccttga gcgcggcgag cgcctgcgct ccggccccgg gacgctgcgg
    23281 caccttcatc gagtagtacg aaactttgcg cacggccatc gtttcctcct tcgctcaggg
    23341 ttgcaggcct ataattctgc gcttctcatg ggccacaggc tatcgaagat caccacgcgc
    23401 accggcgacg ccggcgacac cgggctcggc gacggcacgc gcgtggcgaa ggacagcccg
    23461 cgcatccagg cgctcggcga catcgacgag ctcaattcgt gcctcggcct gctgctggcg
    23521 gagaagccgc ccgcccccat cgccaaagct ctgctgcagg tgcagcacga cctgttcgac
    23581 ctcggcggcg ag
//
LOCUS       DBNHKE010000150         7642 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_977620, whole genome shotgun sequence.
ACCESSION   DBNHKE010000150 DBNHKE010000000
VERSION     DBNHKE010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_977620"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..731)
                     /locus_tag="AC16O9_10205"
     CDS             complement(15..731)
                     /locus_tag="AC16O9_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517029.1"
                     /translation="MSAARPLEVNGVYAAYDKADVLEGVTLSVAPGSITCLLGSNGSG
                     KTTLIRAILGLTPPRAGSIRFGDVELAGLPTHKVIAAGIACIPEGRKVFPKLTVEENL
                     RLGAYQEASAATVAARLAHAYEIFPRLAERRAQLAGTMSGGEQAMVSIGRGLMSAPQL
                     LLVDEPSLGLSPLLVKENFQIIRRINEQGITVFLVEQNVHQTLAIAHHGYVLQQGRVV
                     AAGRAEELARDAAVHQAYFG"
     gene            complement(728..1504)
                     /locus_tag="AC16O9_10210"
     CDS             complement(728..1504)
                     /locus_tag="AC16O9_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517030.1"
                     /translation="MSAPVLLLEGFSRRFGGLVALDQLDMQVGEGEIVGLIGPNGSGK
                     TTCFNVLTGIYEASAGRARFLGRDITNLPAQAVYEAGIARTFQRSRLCLPLSIVDNIL
                     LGNHKNLSRGLWFNLFRRAAFARQFEQAFDEARELVRVFDAPLAERMFEAVGALPMID
                     RRRIEICRALISRPRLLLLDEPSAGMTEEETRELMDDILEIRGRIAGLTVVIIEHEMG
                     VIERITERCVVLNFGRKIAEGSYREVAADRQVQEAYLGVE"
     gene            complement(1501..2646)
                     /locus_tag="AC16O9_10215"
     CDS             complement(1501..2646)
                     /locus_tag="AC16O9_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517031.1"
                     /translation="MSAPLWPGALAAGLVTALAIAFMWAESEWEVVAVLAAAAVGAVL
                     ARRTGWLAPAARAFTEREPAMLRLGIVGLFVFVGFFHDDHFPLLMAGTVLVYFLVCLG
                     LTIQFGYAGMVNFAAAAFLGVGCYTAAVLTTHTAIPHLLVLAIGGAVAALVGSILILP
                     VLRTRGHYSAVVTIAFVLLFKTFLEVNDTLGGPQGLKLPSMQVLGWKFNQNFSVAGVE
                     FSFYVSYVLVALLLVILGFAVTRRLERSWIGLSLDAVRLDETAAACFGVHIARWKIVA
                     FTLGNFMAGVAGALYALMLGFIAPTNFTFGDSLIMVSIILLGGIGNPWGAALAAAIVV
                     MLPEKLQLIQEYRFLLFAALVILVLLYRPGGLLPRRLRVYLPGWKPR"
     gene            complement(2643..3563)
                     /locus_tag="AC16O9_10220"
     CDS             complement(2643..3563)
                     /locus_tag="AC16O9_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517032.1"
                     /translation="MDAGLLAQFVANGLMLGMMYALVAVGFTLFFGVLDVIKFSHGDV
                     LTVGTFAGFSTYLGLQALGAANPWLQVLAVLLVATGSMAILGALIAKYLVMPLKSAPP
                     LNILLVTLMLGTVLRECVRLFFPDGANPKRFPRLLPDDAMALGSFNLRLDSVLLFLAG
                     LAVIVAIHVLINRTKLGLAIRAVAQDEETAKTMGINFTLIVLVTFAVGSAAAAFAGVM
                     NGLYYSEINFGMGLLLGVIGFSAAIVGGLGNIYGAILGGFLFAALQTVGAVALPFASA
                     YKDVFAFAVVIAIMAWRPTGLIAEKTSERV"
     gene            complement(3621..4781)
                     /locus_tag="AC16O9_10225"
     CDS             complement(3621..4781)
                     /locus_tag="AC16O9_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6517033.1"
                     /translation="MKFSRMLFAGALALGLAAGSALAQKKEVKVVFIGPLTGGVSVNG
                     LGGRNSADLAVRLRNADPKAKYKYEMVVLDDECKPNVGVQVATKAASDRSIIAGATHY
                     CSAVAIGTVDVYHKFGLPVIVWGAVLPDITYGNNYKEIHRVNGTMINQNEVNAKFSTG
                     QGFRKFAVIHDTTDYGKGHNKYFSENLAKNGGAIVGTFGVTADQQDFNAELTQIKSLN
                     VDAIYFGGLTPIGVRIRSQMEKLGIKAQFVGTSGIVSDAFIEGLGPLAEGVLAFREGA
                     PVEKLPGGQFFMQKYAEAKYAEAPEAYGAFAFAAMNLILDTIERVGPDRKKVTEALGN
                     VKNVDSIVGKITFDDHGQNTVAAITKYVAQDGKWVVWEDSEYATGKRKLAGK"
     gene            complement(4859..7456)
                     /gene="clpB"
                     /locus_tag="AC16O9_10230"
     CDS             complement(4859..7456)
                     /gene="clpB"
                     /locus_tag="AC16O9_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR6517034.1"
                     /translation="MRLDKLTTRLQEALAEAQSLAVGRDHPYIEPQHLLAALLEQEDS
                     GAASLIAKAGGNPGALKKALAESLGRIAKVEGTAGEVNISRELAALLNVTDKEAQKRG
                     DQYIASELFLLAATADKGEAGRLLKQAGVTKAALEKAIEEVRGGASVSGQADESQRKA
                     LEKYTLDLTDRARAGKLDPVIGRDDEIRRCIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPESLKHKRVLVLDMASLLAGAKYRGEFEERLKAVLKDLAKDEGNTIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLNEYRKYVEKDAALERRFQ
                     KVLVGEPTVEATIAILRGLQEKYEVHHGVEITDPAIVAAAELSHRYVTDRFLPDKAID
                     LIDEAASRIKMEIDSKPESMDRLERRLIQLRIEREAVKKEKDEASKKRLGLIEDEIKR
                     LEKDYSDLEEVWKAEKAQVQGAQHIKEEIERTRQQMAELQRKGQYDKLAELQYGKLPQ
                     LEAQLKSAEKADAKTAKAKLVRTQVGAEEIAEVVSRATGIPVSKMMQGEREKLIKMEA
                     KLHERVVGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALAGFL
                     FDSEEHLIRIDMSDYMEKHSVSRLIGAPPGYVGYDEGGQLTEQVRRKPYAVILFDEIE
                     KAHPDVFNALLQVLDEGRLTDGQGRTVDFKNTVLVMTSNLGSQMIQQMSGSDYGVIKV
                     AVMAEVKNQFRPEFVNRIDEVVVFHALGEEHIKSIAGIQLGVLKARLAKLEYGLEVSD
                     AAVSELAKVGFDPVYGARPLKRAIQSELENPLAKAILEGRFAPKDTIRADWRNGRMSF
                     EKAQARAAA"
BASE COUNT         1187 a         2636 c         2559 g         1260 t
ORIGIN      
        1 ccggccacag gcgctcagcc gaaataggcc tggtgcaccg ccgcgtcgcg cgccagctcc
       61 tcggcgcggc cggcggccac cacccggccc tgctgcagca cgtagccgtg gtgcgcgatg
      121 gcgagcgtct ggtgcacgtt ctgctcgacc aggaacacgg tgatgccctg ctcgttgatg
      181 cggcggatga tctggaagtt ctccttcacc agcagcggcg acaggccgag cgagggctcg
      241 tcgacgagca gcagctgcgg cgcgctcatc aggccgcggc cgatcgacac catcgcctgc
      301 tcgccgcccg acatggtgcc ggcgagctgc gcgcggcgct cggcgaggcg cgggaagatc
      361 tcgtaggcgt gcgcgaggcg cgccgcgacg gtcgccgccg acgcctcctg gtaggcgccc
      421 aggcgcaggt tctcttccac cgtgagcttg ggaaacacct tgcggccctc ggggatgcag
      481 gcgatgccgg cggcgatcac cttgtgcgtc ggcagcccgg cgagctccac gtcgccgaag
      541 cggatcgagc ccgcgcgcgg cggcgtgagg ccgaggatgg cgcggatcag cgtggtcttg
      601 cccgagccgt tcgagccgag caggcaggtg atgctgccgg gggcgaccga gagcgtcacg
      661 ccctcgagca cgtcggcctt gtcgtaggcg gcgtacacgc cgttcacttc gagcggccgc
      721 gcggcgctca ttccaccccc aggtaggctt cctgcacctg gcggtcggcc gccacctcgc
      781 ggtagctgcc ctcggcgatc ttcctgccga agttgagcac cacgcagcgc tcggtgatgc
      841 gctcgatcac gcccatttcg tgctcgatga tgacgacggt gaggcccgcg atgcgcccgc
      901 ggatctccag gatgtcgtcc atcagctcgc gcgtctcttc ctcggtcatg ccggccgagg
      961 gctcgtccag aagcagcagc cgcggccggc tgatgagcgc gcggcagatc tcgatgcggc
     1021 gccggtcgat catcggcagc gcgcccaccg cctcgaacat gcgctcggcg agcggcgcgt
     1081 cgaacacccg caccagctcg cgcgcctcgt cgaacgcctg ctcgaactgg cgcgcgaagg
     1141 cggcgcggcg gaacaggttg aaccacagcc cgcgcgacag gttcttgtgg ttgccgagca
     1201 ggatgttgtc gacgatcgac agcggcaggc acaggcgcga gcgctggaag gtgcgcgcga
     1261 tgcccgcctc gtagaccgcc tgcgccggca ggttggtgat gtcgcggccc agaaaccgcg
     1321 cccgccccgc gctcgcctcg tagatgccgg tgagcacgtt gaagcaggtg gtcttgcccg
     1381 agccgttcgg gccgatgagt cccacgatct cgccctcgcc gacctgcatg tcgagctggt
     1441 cgagcgcgac caggccgccg aagcgccggc tgaagccctc gagcaggagc accggggcgc
     1501 tcatcgcggc ttccagcccg gcaggtacac gcgcagccgc cgcggcagca gcccgcccgg
     1561 gcggtacagc agcaccagaa tcaccagcgc ggcaaacagc aggaaccggt actcctggat
     1621 cagctgcagc ttctcgggca gcatcacgac gatcgccgcc gcgagcgccg cgccccaagg
     1681 gttgccgatg ccgccaagca ggatgatcga caccatgatg agcgagtcgc cgaaggtgaa
     1741 gttggtcggc gcgatgaagc cgagcatcag cgcgtagagc gcgccggcga ccccggccat
     1801 gaagttgccc agggtgaagg cgacgatctt ccagcgcgcg atgtgcacgc cgaagcaggc
     1861 cgccgcggtc tcgtccaggc gcaccgcgtc gaggctcaag ccgatccacg agcgctccag
     1921 gcgccgcgtc acggcgaagc cgaggatcac cagcagcagc gccaccagca cgtagctcac
     1981 gtagaacgag aactcgacgc cggcgacgct gaagttctgg ttgaacttcc agcccagcac
     2041 ctgcatgctg ggcagcttca ggccctgcgg cccgccgagc gtgtcgttca cttccaggaa
     2101 cgtcttgaac agcagcacga aggcgatggt caccaccgcc gagtagtgcc cgcgcgtgcg
     2161 cagcaccggc aggatcagga tcgagccgac cagtgccgcc accgcgccgc cgatggcgag
     2221 caccaaaagg tgcgggatcg cggtgtgcgt agtcagcacc gccgcggtgt agcagcccac
     2281 gccgaggaac gccgcggcgg cgaagttcac catgccggcg tagccgaact ggatggtgag
     2341 cccgaggcag acgaggaaat acaccagcac cgtgcccgcc atcagcagcg ggaagtggtc
     2401 gtcgtggaag aagccgacga acacgaacag ccccacgatg ccgaggcgca gcatcgcggg
     2461 ctcgcgctcg gtgaaggcgc gcgccgccgg cgcgagccag ccggtgcgcc gcgccagcac
     2521 cgcgcccacc gcggcggccg ccagcaccgc caccacctcc cactcgctct cggcccacat
     2581 gaaggcgatg gcgagcgccg tcaccagccc cgcggccagc gcccccggcc agagcggcgc
     2641 gctcatacgc gctcgctcgt tttctcggcg atcaggccgg tcgggcgcca ggccatgatc
     2701 gcgatcacca cggcgaaggc gaacacgtcc ttgtaggcgc tggcgaaagg cagcgccacc
     2761 gcgccgacgg tctgcagcgc ggcgaacagg aacccgccca ggatcgcgcc gtagatgttg
     2821 ccgagacccc cgacgatcgc cgcgctgaag ccgatcacgc cgagcagcag gcccatgccg
     2881 aagttgatct cgctgtagta caggccgttc atcacgccgg cgaaggccgc cgccgccgag
     2941 ccgaccgcga aggtcaccag cacgatgagc gtgaagttga tgcccatggt cttcgccgtc
     3001 tcctcgtcct gcgccacggc gcggatcgcg agaccgagct tggtgcggtt gatgagcacg
     3061 tggatggcga cgatcaccgc caggccggcg agaaacagca gcacgctatc caggcgcagg
     3121 ttgaagctgc cgagcgccat cgcgtcgtcg ggcagcaggc gcggaaagcg cttcgggttg
     3181 gcgccgtcgg ggaagaacaa gcgcacgcac tcgcgcagca ccgtgccgag catcagcgtc
     3241 accagcagga tgttgagcgg cggcgcggat ttcagcggca tgacgagata cttggcgatg
     3301 agcgccccga ggatcgccat cgagccggtg gccaccagca gcacggccag gacctgcagc
     3361 cagggattgg cggcgccaag cgcctgcagg ccgaggtagg tcgagaagcc ggcgaaggtg
     3421 cccaccgtga gcacgtcgcc gtggctgaac ttgatgacgt cgagcacgcc gaagaacagc
     3481 gtgaagccga ccgcgaccag ggcgtacatc atgcccagca tcaggccgtt ggcgacgaat
     3541 tgcgcgagga gtccggcgtc catgcgaaat ggaaggggca ggcgctgcgc ccgccccttc
     3601 cgccagtcag gactgcctgc ttacttcccg gcgagcttgc gcttgccggt ggcgtattcg
     3661 ctgtcctccc agaccaccca cttgccgtcc tgggcgacgt acttggtgat cgccgccacg
     3721 gtgttctggc cgtggtcgtc gaacgtgatc ttgccgacga tcgaatccac gttcttcacg
     3781 ttgccgagcg cctcggtgac cttcttgcgg tcggggccga cgcgctcgat ggtgtcgagg
     3841 atcaggttca tggcggcgaa ggcgaacgcg ccgtaggcct cgggcgcttc cgcgtacttc
     3901 gcctcggcgt acttctgcat gaagaactgc ccgccgggga gcttttccac cggcgcgccc
     3961 tcgcggaacg cgagcacgcc ctcggcgagc ggccccaggc cctcgatgaa cgcatccgag
     4021 acgatgcccg aggtgcccac gaactgcgcc ttgatgccga gcttttccat ctgcgagcgg
     4081 atgcgcacgc cgatcggggt gagacccccg aagtagatcg cgtccacgtt gagcgacttg
     4141 atctgcgtga gctcggcgtt gaagtcctgc tggtcggcgg tgacgccgaa ggtgccgacg
     4201 atcgcgccgc cgttcttcgc caggttctcg ctgaagtact tgttgtggcc cttgccgtag
     4261 tcggtggtgt cgtggatcac ggcgaacttc ctgaacccct ggccggtgga gaatttcgcg
     4321 ttcacctcgt tctggttgat catcgtgccg ttgacgcggt ggatctcctt gtagttgttg
     4381 ccgtaggtga tgtcgggcag caccgcgccc cacacgatca ccggcaggcc gaacttgtga
     4441 tagacgtcga cggtgccgat cgccaccgcc gagcagtagt gcgtggcgcc ggcgatgatc
     4501 gagcggtcgg aggcggcctt ggtcgccacc tgcacgccga cgttcggctt gcactcgtcg
     4561 tcgagcacca ccatctcgta cttgtacttc gccttggggt cggcgttacg cagccgcacc
     4621 gcaaggtcgg cggagttgcg cccgcccagc ccgttcaccg aaacgccgcc ggtaagcggg
     4681 ccgatgaaca ccactttgac ttccttcttc tgcgcgagcg cgctgccggc ggcaaggccg
     4741 agcgcgagcg cgccggcaaa cagcatgcgt gagaacttca tggatcccct cctcctggaa
     4801 tgatcgcaag cgacggcgcg agcttgcgcc atcgcgcggt gagggtcaag acggtgtttt
     4861 acgccgccgc gcgcgcctgc gccttctcga agctcatgcg cccgttgcgc cagtcggcgc
     4921 ggatggtgtc cttgggcgca aagcgtccct ccagaatcgc cttggcgagc gggttctcga
     4981 gctcggactg gatggcgcgt ttcagcggcc gcgcgccgta caccgggtcg aagccgacct
     5041 tggcgagctc gctcaccgcc gcgtccgaca cctccagccc gtactcgagc ttcgccaggc
     5101 gcgccttcag cacgccaagc tggatgccgg cgatcgactt gatgtgctcc tcgcccaggg
     5161 cgtggaacac caccacctcg tcgatgcggt tgacgaactc gggccggaac tggttcttca
     5221 cctcggccat caccgccacc ttgatcacgc cgtagtccga cccggacatc tgctggatca
     5281 tctgcgagcc gaggttcgag gtcatgacga gcaccgtgtt cttgaagtcg accgtcctgc
     5341 cctggccgtc ggtcaggcgc ccctcatcga gcacctgcaa gagcgcgttg aagacgtcgg
     5401 ggtgcgcctt ctcgatctcg tcgaagagga tcacggcgta gggcttgcgg cgcacctgct
     5461 cggtgagctg cccgccctcg tcgtagccca cgtacccggg cggcgcgccg atgaggcgcg
     5521 acaccgagtg cttctccatg tagtccgaca tgtcgatgcg gatgaggtgt tcttccgaat
     5581 cgaagaggaa accggcgagc gccttgcaca gctcggtctt gcccacgccc gtgggcccga
     5641 ggaacaggaa cgagccgtac gggcggttcg ggtcggaaag gcccgagcgc gagcgccgta
     5701 tcgcatccga cacgaggcgc acggcctcgt cctggccgac cacgcgctcg tgcagtttcg
     5761 cttccatctt gatgagcttc tcgcgctcgc cctgcatcat cttggacacg ggaatgccgg
     5821 tggcgcgcga caccacctcg gcgatctctt ccgcgccgac ctgggtgcgc acgagcttcg
     5881 ccttcgccgt cttggcgtcg gccttctccg cgctcttcag ctgcgcttcg agctgcggca
     5941 gcttgccgta ctgcagctcg gcgagcttgt cgtactggcc ttttcgctgc agctcggcca
     6001 tctgctggcg cgtgcgctcg atctcttcct tgatgtgctg cgcgccctgc acctgggctt
     6061 tctccgcctt ccagacctct tccaagtcgg aatagtcctt ctccaggcgc ttgatctcgt
     6121 cctcgatgag gccgaggcgc ttcttcgacg cttcgtcctt ttccttcttc accgcctcgc
     6181 gctcgatcct gagctggatc aggcgccgct cgagccggtc catcgactcg ggcttggagt
     6241 cgatctccat cttgatgcgc gaggcggcct cgtcgatcag gtcgatcgcc ttgtccggga
     6301 ggaaccggtc ggtgacgtag cggtgcgaga gctcggccgc ggcgacgatc gccgggtccg
     6361 tgatctcgac gccgtggtgc acctcgtact tctcctgcag gccgcgcagg atggcgatgg
     6421 tcgcctcgac cgtcggctcg cccaccagca ccttctggaa gcgccgctcg agcgcggcgt
     6481 ccttttccac gtacttgcgg tattcgttca gggtggtggc gccgatgcag tgcagctcgc
     6541 cgcgcgcgag cgcgggcttg agcatgttgc cggcgtcgat cgcgccttcg gccttgccgg
     6601 cgccgaccat ggtgtgcagc tcgtcgatga agacgatggt gttgccctcg tccttcgcca
     6661 ggtccttcag caccgccttg aggcgctcct cgaactcgcc gcggtacttg gcgccggcga
     6721 gcagcgaagc catgtcgagc accagcacgc gcttgtgctt cagcgactcg ggcacctcgc
     6781 cattgatgat ccgttgcgca aggccctcca cgatggcggt cttgcccacg cccggttccc
     6841 cgatcagcac aggattgttc ttcgtgcgcc gctgcaggat ctggatgcag cgccggatct
     6901 cgtcgtcccg gccgatgacc gggtcgagct tgcccgcgcg cgcgcggtcg gtgagatcga
     6961 gcgtgtattt ttcgagggcc ttcctctgcg actcgtcggc ctggccggaa acgctcgccc
     7021 cgccgcgcac ttcctcgatc gccttctcca gcgccgcctt cgtgacgccg gcctgcttca
     7081 gcagccggcc ggcttcgccc ttgtcggccg tggcagcgag cagaaagagc tccgaggcga
     7141 tgtactggtc gccgcgcttc tgcgcctcct tgtcggtgac gttgagcagc gccgccagct
     7201 cgcgcgagat gttgacctcg cccgcggtgc cttccacctt ggcgatgcgc ccaagcgact
     7261 cggcgagggc cttcttcagc gcgccggggt tgccgcccgc cttggcgatg agcgaggcgg
     7321 caccgctgtc ctcctgctcg aggagcgcag ccagtaggtg ctgcggctcg atgtacgggt
     7381 ggtcgcgccc cacggcgagg ctctgggcct cggcgagggc ttcctgcagc cgggtggtca
     7441 atttgtcgag tcgcatgtgg ggagaatggg ggcggaagcc cccgattcaa gcgaggaacc
     7501 ggtagcttgc gtagcccacg agggtggcca gcagcgcccc cagcaggtgg gcgcccgagt
     7561 gcgccagggc cgcgaggtac ctttccgcgt acagcagcgc catggactcg gccgaaaacg
     7621 tggagaaggt ggtgagtgcg cc
//
LOCUS       DBNHKE010000151         7056 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_925421, whole genome shotgun sequence.
ACCESSION   DBNHKE010000151 DBNHKE010000000
VERSION     DBNHKE010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_925421"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC16O9_10235"
     CDS             <1..370
                     /locus_tag="AC16O9_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6517035.1"
                     /translation="IGINTAKNYLMALGRRAPTSTEVEAEDAEGFEDGEQLRDINTPE
                     SVLLSAEIARTVNATIDELPEELRTAIQLREIEGMSYEDIARIMDCPIGTVRSRIFRA
                     REAIAERLRPLLDTRKDQRW"
     gene            375..923
                     /locus_tag="AC16O9_10240"
     CDS             375..923
                     /locus_tag="AC16O9_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR6517036.1"
                     /translation="MKAKISALMDGELDALEQGEPMAALGTDAEALETWRTYHLISDA
                     LQGRAQLAHDCLRRVMARLAEEPALIGPLPADVVPPERSRWFAPSALAAGVAAVAIVG
                     WMAFAPPQGAAPLPAPVAEGPAPTAVTMARRATEAPVRLPMTEATRDYLIAHQVFSPR
                     SSLQGMAPYVRSVAAESHPGKP"
     gene            920..1876
                     /locus_tag="AC16O9_10245"
     CDS             920..1876
                     /locus_tag="AC16O9_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR6517037.1"
                     /translation="MSRTVCALLLFAVAGWAQAQTTPEALEWLRKIYQASEQLSYSGT
                     FVYQQRDRTETSRITRLAGVDGGTEKLEVLDGAPREILRTRDEIRCYLPESRTVKVDR
                     RSDPRAFPALLPERVDRLAEYYRVSMEGTERIAGFKGRVIALSPKDDLRYGYRLWADA
                     DSGMLLKAQTFDREGQTMEQFTFTHLEIGSVPRERLRPPHAAQGWRVEQTAVAPVDLS
                     RSGWSVGAELPGFRKIIEIRRMLRAAQPVAQMVYSDGLAAVSVFIEPMSGRPEAARFG
                     LSSLGAVNVYTREVADHLVTVVGETPAASVQRIGNTVVYRPQ"
     gene            1909..3327
                     /locus_tag="AC16O9_10250"
     CDS             1909..3327
                     /locus_tag="AC16O9_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR6517038.1"
                     /translation="MLLIRAAAVLAFGTVATFVSAQGRDLPDFTHLVEAHGAAVVNVS
                     TTQAPRRASTPQVPNIEDEEMLEFFRRFIPRQPGPGSRPESRSLGSGFIISPDGYILT
                     NAHVIDAADEIDVRLTDKREFKARVIGADKRTDVALIKIEASNLPAVRMGDPSRLKVG
                     EWVVAIGSPFGFENTVTAGIVSAKGRSLPQENFVPFIQTDVAINPGNSGGPLFNMKGE
                     VVGVNSQIYSRTGGFMGLSFAIPIDVALDIQSQLREKGRVSRGRIGVVIQEVTKDLAV
                     SFGLQKPHGALVNSVEKGSPADKAGIEATDIITRFDGKEVDSSSDLPRIVGGTRPGSQ
                     VQVEVWRKGASRLLSITVGELLEERIAAREAPREPKPAPLAANRLGVVVSELSAEQKR
                     ELKLAHGLVVAEVRPNARAELRRGDILLKLVYNGRHTDLKTTEQLNKLLAGLDKNAVI
                     TLQVRRGDSTAFVSVAGLGDKG"
     gene            3293..3556
                     /locus_tag="AC16O9_10255"
     CDS             3293..3556
                     /locus_tag="AC16O9_10255"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6517039.1"
                     /translation="MSPSPAWATRAEPILRLYSRANCHLCDEMAEALRALGVEFAKRD
                     VDADPALAERYGSKVPVLTDGADREICHARLDLRALRARLALE"
     gene            3602..5404
                     /gene="lepA"
                     /locus_tag="AC16O9_10260"
     CDS             3602..5404
                     /gene="lepA"
                     /locus_tag="AC16O9_10260"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR6517040.1"
                     /translation="MTPQGRIRNFSIIAHIDHGKSTLADRIIQRCGGLAAREMADQVL
                     DSMALERERGITIKAQTAALEYLARDGERYLLNLIDTPGHVDFSYEVSRSLAACEGAI
                     LVVDASQGVEAQTVANCYTAIEQGVEVIPVLNKIDLPAADPDNAIQEIEDVIGIEAQD
                     ALRASAKTGEGVDEILEAIVARVPPPKGEPTARLQALVIDSWFDNYVGVVMLVRMMQG
                     TLRPKDKVLLMSADAQHLCEQVGVFTPKSQPREALGAGEVGFVIAGVKDLREARVGDT
                     LTHADKPAAQPLPGFKEIKPQVFAGLYPVEANQYEALREALGKLHLNDASFHYEPEIS
                     QALGFGFRCGFLGLLHMDIVQERLEREYGMQLVTTAPSVVYEVLLRDGSVEQVSNPAK
                     LPDPGQIEEIREPVIATTIFVPQEHVGPVMTLCTEKRGVQTNLHYAARHVVLSYDLPL
                     NEVVLDFFDRLKSMTRGFGSMDYDFKEYRPADVVRLDVLVNGERVDALSSMVHRDVAQ
                     RRGRELAARLRGLIPRQMFDVAIQAAIGGNVVARENVKALRKNVLAKCYGGDVTRKRK
                     LLEKQKEGKKRMKQVGSVEIPQEAFLAVLQRDAD"
     gene            5407..6195
                     /gene="lepB"
                     /locus_tag="AC16O9_10265"
     CDS             5407..6195
                     /gene="lepB"
                     /locus_tag="AC16O9_10265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6517041.1"
                     /translation="MSFALFLLLLLVLTGAVWALEAAWLRKRRGADAKQPWWVEYSVS
                     FFPVILIVFLLRSFLVEPFKIPSGSMIPTLLVGDFILVNKFTYGIRLPVANRKLLEVG
                     SPQRGDVMVFRYPEDPSLDYIKRVVGVPGDRVEYRNKRLSINGRPVPVRQIEDYLSRE
                     RMQYSRRYLETLNGAEHQIMIEEDAPAYVPQVRAFPHAGNCNYNMSGLACTVPPGHYF
                     VMGDNRDNSSDSRVWGFVPDGNIVGKAFFIWLNLGELGRFGAFR"
     gene            6222..6578
                     /locus_tag="AC16O9_10270"
     CDS             6222..6578
                     /locus_tag="AC16O9_10270"
                     /inference="COORDINATES: protein motif:HMM:NF027463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR6517042.1"
                     /translation="MRARQRGITLMGLIMGSFVLVFVALLAMRLLPSYIEYFTIKKAV
                     VSISNEMRGRGGSANDVRRAFDARQAIDDFKAVKAADLEITKVGNDVQIVAAYRKEIP
                     LFANIGIYIDFVAESN"
     gene            6598..>7056
                     /locus_tag="AC16O9_10275"
     CDS             6598..>7056
                     /locus_tag="AC16O9_10275"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:NF025973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III family protein"
                     /protein_id="HIR6517043.1"
                     /translation="MSRTARLEERLGHRFRHGELLEQALTHRSFGTPHNERLEFLGDG
                     VLGCVIAEALLARFPQLSEGELSRMRASLVRQEALAAAAASLELAGYLRMGQGESARG
                     GAQRPSILADALEALFGAVFLDGGYASARAAVLATLGGALAATDELTTAKD"
BASE COUNT         1167 a         2297 c         2480 g         1112 t
ORIGIN      
        1 cattggcatc aacaccgcca agaattatct gatggcgctg gggcggcggg cgccgacctc
       61 caccgaggtg gaggcggagg acgccgaggg tttcgaggac ggcgagcagt tgcgggacat
      121 caatacgccg gaaagcgtat tgctgtcggc ggagatagca cggacggtga atgcgacgat
      181 cgacgaattg cccgaggagt tgcgcacggc gatccagctg cgcgagatcg agggcatgag
      241 ctacgaggat atcgcgcgca tcatggactg tccgatcggg acggtgcggt cgcggatttt
      301 cagggcgcgc gaggcgattg cggagcggct gcggcctctg ctcgatacgc gcaaggacca
      361 gaggtggtag cgctatgaaa gccaagattt cggcattgat ggacggtgag ctcgatgcgc
      421 tcgagcaagg cgagccgatg gcggcgctgg gcacggacgc cgaggcgctc gagacctggc
      481 gtacctatca tctgatcagc gacgcgctgc aggggcgcgc ccagctggcg cacgactgcc
      541 tgcgccgcgt catggcccgc ctggccgagg agccggcgct gatcgggccg ctgccggcgg
      601 acgtggtacc gccggaacgt tcccgctggt tcgcgccctc cgcgctggcg gcgggcgtgg
      661 cggcggtggc gattgtcggg tggatggcgt tcgcgccgcc gcagggcgcg gcgcccctgc
      721 cagcacccgt ggccgagggg ccggcgccga ccgcggtgac catggcgcgg cgggcgaccg
      781 aagcgccggt gcgcctgccg atgaccgaag cgacgcgcga ctacctgatt gcgcaccaag
      841 tgttctcgcc gcgcagttct ctgcagggca tggcgcccta tgtgcgctcg gtagccgccg
      901 aatcgcaccc gggcaaaccg tgagccgtac cgtctgcgct ctgctgcttt ttgcggtcgc
      961 cgggtgggcg caggcacaga ccacgcccga agcgctcgag tggctgcgca agatctacca
     1021 ggcctccgag cagctctcgt actcgggcac cttcgtctat cagcagcggg accgcaccga
     1081 gacctcccgc atcacgcgtt tggcgggcgt cgacggcggc accgaaaagc tggaagtgct
     1141 ggacggcgcg ccgcgcgaga ttctgcgcac gcgagacgag atccgctgct atttgccgga
     1201 aagccgcacg gtgaaagttg accgtcgcag cgatccgcgt gctttcccgg cgctgctgcc
     1261 ggaacgggtc gaccgcctcg ccgagtacta cagggtgagc atggagggga cggagcgtat
     1321 cgccggcttc aagggccggg tcatcgcgct gtcccccaag gatgatctgc gctacggcta
     1381 ccgcctgtgg gccgacgcgg acagcggcat gctgttgaag gcgcagacct tcgaccgcga
     1441 agggcagacg atggagcagt tcactttcac gcacctggag atcggcagcg tgccgcgcga
     1501 gcgtctgcgc ccgccgcacg ccgcgcaggg ctggcgcgtg gaacagaccg cggtggcccc
     1561 ggtggatctg tcgcgctcgg gatggagcgt cggcgcggag ctgcccgggt tccgcaagat
     1621 catcgagatc cggcgcatgc tgcgtgccgc ccagccggtg gcgcagatgg tgtactcgga
     1681 cggcctcgcc gccgtgtcgg tgttcattga gccgatgtcc ggccgacccg aggcagcgcg
     1741 cttcggcctg tccagcctgg gggcggtcaa cgtctacacg cgtgaggtcg ccgaccacct
     1801 cgtcaccgtc gtaggcgaaa cgccggccgc gagcgtgcag cgcatcggca acacggtcgt
     1861 gtaccgcccg caatagcagt tgtcgcccac cctccgagag gattcttcat gctactgatc
     1921 cgagccgccg ccgtgctggc gttcggcacc gttgccacgt ttgtcagcgc ccaggggcga
     1981 gacctgcccg acttcacgca cctggtagag gcgcacggtg ccgccgtcgt caatgtcagc
     2041 acgacgcagg cgccgcggcg cgcgagcacg ccgcaggtgc cgaacatcga ggatgaggag
     2101 atgctggagt tcttccggcg cttcattcca cggcaaccgg ggcccgggtc gcggcccgaa
     2161 agccggtccc ttggctcggg gttcatcatc agccccgacg gctacatcct taccaatgcg
     2221 cacgtcatcg atgccgcgga cgagatcgat gtgcgcctca ctgacaagcg cgaattcaag
     2281 gcccgggtca tcggcgccga caagcgcacc gacgttgcgc tgatcaagat cgaggcgagc
     2341 aacttgccgg cggtgcgcat gggcgacccc tccaggctca aggtggggga atgggtggtg
     2401 gcgatcggct cgcctttcgg cttcgagaat acggtgaccg ccggcattgt cagcgccaag
     2461 ggccgctcgc tgccgcagga gaacttcgtg ccattcatcc aaacggacgt cgcgatcaac
     2521 ccgggcaatt ccggcgggcc gctgttcaac atgaagggtg aagtcgtcgg cgtgaattcg
     2581 cagatctaca gccgcaccgg cggattcatg ggcttgtcgt tcgccatccc gatcgacgtc
     2641 gcgctcgaca tccagagcca gctgcgcgag aaaggccgtg tctcgcgcgg ccgcatcggc
     2701 gtcgtgattc aggaggtgac caaggacctg gcagtgtcgt ttggcctgca gaagccgcac
     2761 ggggcgctgg tcaattcggt cgagaagggc agcccggcgg acaaggcggg catcgaggcc
     2821 acggacatca tcacgcgctt cgacggcaag gaggtagatt cctccagcga cctgccgcgc
     2881 atcgtcggcg gcacgcgtcc gggaagccag gtgcaggtcg aggtgtggcg caagggggcg
     2941 tcgcgattgc tcagcatcac ggtgggcgag ctgctcgagg agcggatcgc ggcgcgcgaa
     3001 gcgccgcgcg agccgaaacc cgcgccgctc gccgccaacc gcctcggggt ggtggtgagc
     3061 gagctgagcg ccgagcagaa gcgggaactg aagctggcgc acggcctggt ggtcgccgag
     3121 gtgcggccca acgcacgcgc cgagctgcgc cgtggcgaca tcctgctcaa gctcgtgtac
     3181 aacggccggc acaccgatct aaagaccacc gagcagctga acaagctgct cgcggggctc
     3241 gacaagaacg ccgtgatcac gctccaggtg cgccgcggag atagcacggc gtttgtctcc
     3301 gtcgccggcc tgggcgacaa gggctgagcc gattctgcgc ctgtactcgc gcgcgaactg
     3361 ccacctctgc gacgagatgg cggaggcatt gcgcgccctc ggcgtcgagt tcgccaagcg
     3421 cgacgtggat gccgatccgg cgctcgcgga acgctacggc agcaaggtcc ccgtgctgac
     3481 cgacggcgcg gaccgcgaga tctgtcacgc ccggctcgat ctgcgggccc tgcgtgcgcg
     3541 gcttgcgcta gaatagcggc cgctcgagac cgcgaagggt gccgccggca cccttttttt
     3601 catgacgccg caaggccgca tccgcaactt ttccatcatc gcgcacatcg atcacggcaa
     3661 gtcgacgctc gctgatcgca tcatccagcg ctgcggcggc cttgcggcgc gcgagatggc
     3721 cgaccaggtg ctcgattcga tggcgctcga gcgcgagcgt ggcatcacca tcaaggcgca
     3781 gaccgcggcg ctggaatacc tggcgcgcga cggcgagcgc tacctgctca acctgatcga
     3841 taccccgggc cacgtcgact tctcctatga agtcagccgc tccctggcgg cctgcgaggg
     3901 tgcgatcctg gtggtcgacg cgtcgcaggg cgtcgaggcg cagacggtgg ccaactgcta
     3961 cacggcgatc gagcagggtg tcgaggtgat cccggtgctg aacaagatcg acctgcccgc
     4021 ggccgacccg gacaacgcga tccaggagat cgaggacgtc atcggcatcg aggcacagga
     4081 cgcgctgcgg gcgagcgcca agaccggcga gggggtggat gaaatactcg aggcgatcgt
     4141 cgcgcgcgtg ccgccgccca agggcgagcc gacggcgcgg ctgcaggccc tggtcatcga
     4201 ctcctggttc gacaactacg tcggggtggt gatgctggtg cgcatgatgc agggaacgct
     4261 ccgccccaag gacaaggtgc tcctgatgag cgcggacgcg cagcacctgt gcgagcaggt
     4321 gggcgtgttc acgcccaagt cgcagccgcg tgaggccctg ggcgcgggcg aggtcggatt
     4381 cgtcatcgcc ggcgtgaagg acctgcgcga agcgcgggtc ggcgataccc tgacacacgc
     4441 cgacaagccc gcggcgcagc cgctgcccgg cttcaaggaa atcaagccac aggtgttcgc
     4501 cggcctgtac ccggtcgaag ccaaccagta cgaggcgctg cgcgaggcgc tcggcaagct
     4561 gcacctcaac gacgcctcgt tccactacga gccggagatc tcgcaggcgc tcggcttcgg
     4621 ctttcgctgc ggtttcctcg gcctgctgca catggatatc gtgcaggagc ggctggagcg
     4681 cgagtacggc atgcagctgg tcacgaccgc gccgtcggtg gtgtacgagg tcttgctgcg
     4741 cgacggctcg gtggagcagg tctccaatcc ggccaagctg cccgaccccg gccagatcga
     4801 ggaaatccgc gagccggtca tcgccaccac gatcttcgtg ccgcaggagc atgtcggccc
     4861 ggtgatgaca ctgtgcaccg agaagcgcgg cgtgcagacc aatctgcact acgcggcgcg
     4921 gcacgtggtt ctgtcgtacg accttccgct gaacgaggtg gtgctggact tcttcgaccg
     4981 gctgaaatcg atgacgcgcg gcttcggctc catggactac gacttcaagg agtaccgtcc
     5041 cgccgacgtg gtgcggctcg acgtgctggt gaacggcgag cgcgtggacg ctctgtccag
     5101 catggtgcac cgcgacgtcg cgcagcgccg cggccgcgag ctcgcggccc ggctgcgcgg
     5161 cctgatcccg cgccagatgt tcgatgtcgc cattcaggcc gccatcggcg gcaacgtcgt
     5221 ggcgcgcgag aacgtgaagg cacttcgcaa gaacgtactc gccaagtgct acggcgggga
     5281 cgtgacgcgc aagcgcaagc tcctggagaa gcaaaaggag ggcaagaagc gcatgaagca
     5341 ggtgggctcg gtggagattc cccaggaggc gttcctggcg gtgctgcagc gggacgcgga
     5401 ctaggcatga gcttcgccct gttcctgctg ctgctgcttg tcctgacggg cgcggtctgg
     5461 gcgctggagg cggcgtggct gcgcaagcgt cgcggggcgg acgccaagca gccgtggtgg
     5521 gtcgagtatt cggtgagctt cttcccggtg atcctcatcg tgttcctgct gcgctcgttc
     5581 ctggtcgagc cgttcaagat cccctcgggt tcgatgatcc cgacgctgct cgtgggcgat
     5641 ttcatcctcg tgaacaagtt cacctacggc atccggctgc cggtggcgaa ccgaaagctg
     5701 ctcgaggtgg gcagcccgca gcgcggcgac gtcatggtgt tccggtatcc cgaggatccg
     5761 tcgctggatt acatcaagcg ggtcgtcggc gtgccgggcg atcgcgtcga gtaccgcaac
     5821 aagcgcctga gcatcaacgg ccgccccgtg ccggtgcgcc agatcgagga ctacctgtcg
     5881 cgcgagcgca tgcagtactc gcggcgctac ctggaaacgc tcaacggcgc cgagcaccag
     5941 atcatgatcg aggaggacgc gccggcctac gtgccgcagg tgcgcgcgtt cccccacgca
     6001 ggcaattgca actacaatat gtccggcctg gcgtgcacgg tgccgccggg gcactacttc
     6061 gtgatgggtg acaaccgtga caactcgagc gacagccggg tgtgggggtt cgtgcccgat
     6121 ggcaatatcg tcggcaaggc gttcttcatc tggctcaacc tcggcgagct ggggcgcttc
     6181 ggcgctttcc gctgagggct cgctgcaaca ggagatcggc catgcgcgct aggcaacggg
     6241 gcataacgct gatggggctc atcatgggct ctttcgtcct cgtgttcgtg gcattgctcg
     6301 cgatgcggct gctgccgtcg tacatcgagt acttcaccat caagaaagcg gtggtctcga
     6361 tttccaacga gatgcgcggg cgcggcggct cggccaacga cgtgcgccgc gcgttcgacg
     6421 cccggcaggc gatcgacgat ttcaaggcgg tcaaggcggc cgacctcgag atcaccaagg
     6481 tgggcaacga cgtgcaaatt gtcgccgcct accgcaagga gatcccgctg ttcgccaaca
     6541 tcggcatcta catcgacttc gtggccgagt ccaactagcc ggggccgcgc acgcgccttg
     6601 tcacgcaccg cccgcctcga ggaacgcctc ggccatcggt tccgccacgg cgagctgctc
     6661 gagcaggcgc tgacgcaccg cagcttcggc accccgcaca atgagcgtct cgagttcctc
     6721 ggcgacggcg tgctcggctg cgtaatcgcc gaggcgctgc tggcgcggtt cccgcaactg
     6781 agcgagggcg agctttcgcg aatgcgcgcc agcctggtgc gccaggaggc gctggcggcc
     6841 gcggcggcca gcctcgagct cgccgggtac ctgcgcatgg gccagggcga gagcgcgcgc
     6901 ggcggcgcgc agcgtccctc gatcctcgcc gatgcgctcg aggcgctgtt tggcgcggtg
     6961 tttctcgacg gcggctacgc cagcgcccgg gccgccgtcc tcgccacgct cggcggggcg
     7021 ctcgcggcga ccgatgagct cacgactgcc aaggac
//
LOCUS       DBNHKE010000152         7322 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_555184, whole genome shotgun sequence.
ACCESSION   DBNHKE010000152 DBNHKE010000000
VERSION     DBNHKE010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_555184"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="AC16O9_10280"
     CDS             <1..1977
                     /locus_tag="AC16O9_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6517044.1"
                     /translation="FKPLDLGFVTLPNRILMGSMHTGLEARPDGMERLAAFYAERAMG
                     GAALIVTGGFSPNDAGELGPHRAQVSTKADAERHKVVPRAVHEAGGRIALQVLHSGRY
                     GFHKNTVAPSAVKSGINPNAPREMAPDEIQKTIAAFAASAALAREAGYDGIEVMGSEG
                     YLISQFLAPRVNQRADDWGGSLENRMRFSVEVVRACRVAAGEDFILLYRISALELVEG
                     GLSAEEIAKVAKAIEKAGATLLNTGIGWHEARIPTIAQAVPRGGFAWATRNLKQAVKI
                     PVIASNRINAPEVAEAILARGDADMVSLARAFLADEAFAAKAKAGDRAGINICIACNQ
                     ACLDHYFIGQPASCVVNPRAGRETKLVYAPAKKKKRLAVVGGGPAGLSCAAVAAERGH
                     EVVLYDKAAALGGQFNLAKRIPGKQEFAESIAYYAERIRRAGAKVVLNHAPGEAELAG
                     FDEVILATGIEPRRPDIPGIDHRSVVGYVDLLSGRVAPGKNVVIIGAGGIGFDVALYL
                     LEKNERATLEPDAFAKHWGIVGDRAAAGGLDPKGPPQAHPAHKITMLKRSAGPFGTTL
                     GRTTGWVHRAELARNGVAMLKGVEYRRIDDAGVHIAIDGKEQVVAADTVVICAGQEPL
                     RPLAAKHVIGGAKLAAELDAKRAMLEGAELAAAL"
     gene            1978..2841
                     /gene="yddG"
                     /locus_tag="AC16O9_10285"
     CDS             1978..2841
                     /gene="yddG"
                     /locus_tag="AC16O9_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid exporter YddG"
                     /protein_id="HIR6517045.1"
                     /translation="MNAATLAGGAAVLLWSTLALLTAGATRIPPLQLVFLTFAVASAI
                     GLAYALLRRVNVFRAARRHPGALALSTAALGGYHFFYFVALANAPVVDASLIAYLWPL
                     LIVVLAAAVGGARVTRWHFLGAVLGLAGAALVIAARGALAASPQFLAGYGAALACAFI
                     WSGYSVANRRHAGAPTELVALACALVALLALPAHLYFETFALPSDREWPSILLLGAGP
                     VGAAFFLWDYGTKHGRIALLGALSYAAPVLSTAFLVAAGDAAPTWHIGAGCVLVAGGA
                     ALAAYGERRVG"
     gene            2852..3580
                     /locus_tag="AC16O9_10290"
     CDS             2852..3580
                     /locus_tag="AC16O9_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517046.1"
                     /translation="MDKPPVAVVAGVGEGLGFALGKRFAQAGFNVALAARSAERLARL
                     AGEIQKAGGKAFAAPTDVREEQEVMALFDALESEHGPVQVAVFNAGANFRASILETPA
                     DMFEKIWRLGCHAGFLFGREAARRMTPHGAGTILFTGATASIRGSSHFAAFAAAKNGL
                     RAVAQSMARELGPKGIHVAHVVIDGMIDTAAVRARFPDRVKDLGVDALLRTDAIAELY
                     YQIHAQPRSAWTFEADLRPFGEKF"
     gene            3582..4523
                     /locus_tag="AC16O9_10295"
     CDS             3582..4523
                     /locus_tag="AC16O9_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6517047.1"
                     /translation="MQAADAQRLFERIAANIESVMRGQHAQVRKLLAAFAAGGHVLVD
                     DFPGTGKTTLAKSLAASIGAPFARVQFTPDLLPSDILGVSVFNQRDQSFEFREGPVFT
                     SILLADEINRASPRTQSALLEVMAEGQVSVEGRTHAMDPMFFVVATQNPVEFRGTYPL
                     PEAQMDRFALCLALGYVDADTEVAVLSAQDLGHPLERIGPVAAREEVLALKEAVRRVR
                     VAEELKHYVVELVRRTRAAPQVLLGCGPRASIALTHTAKALALFDGEEFVRPEHVQEL
                     AVATLAHRMVLDPQARFAGASAASLVGELLREVKVPA"
     gene            4520..5866
                     /locus_tag="AC16O9_10300"
     CDS             4520..5866
                     /locus_tag="AC16O9_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6517048.1"
                     /translation="MRRPIYLALRVFSALDHWLRERLTAAGWLALGAAGAAGAAGLDT
                     TQNTSYQAATLLGALLLLAWAVSLFFRARVEVRRELPRYATAGEPFSYVVAVENQGPR
                     ALAGLTLRERLADPRPRYDEWRRAREPGEERRNWFDRNVGYFRWRWLIERRTPQPPRE
                     SPLPELAPGARRALRLTLTPRRRGRIELAGLVLGRTDPLGLVRGLAPLPLAAHITALP
                     RRYRLPRLALPGRRRHQPGGVSLASSVGDSEEFLALREYRPGDPLQHIHWKSFARTGR
                     PIVKEFQDEFFERHALVLDTGTVRGEDSAFEDAVAVAASFVYSIDTQECLLDLLFVGG
                     EVRHYVAGRGQLQSEHMLEVLAGVGPSAPQGFDALSRAVLEARTQLASCILVLVSWDD
                     ARRRLAESLAASGTEVRAILVCASEDKPRDAPAWLTAVHPGAIEAGLAGLERRVLR"
     gene            5863..>7322
                     /locus_tag="AC16O9_10305"
     CDS             5863..>7322
                     /locus_tag="AC16O9_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR6517049.1"
                     /translation="MSLPPFIAGAALLFWGWHSGNLLVAAPLALALEAPRWLRLRFAL
                     GPPDYARIADLCTVFFVGLAVALAFDRGVAHGVIGAFRWLPVVLAPILLAQRLSEDNR
                     VPLTALFRYLRKQKQRNPELQVPLVDTGGVFVVLCVVAAGVGNAATQTYYAGAVLLAA
                     WALFATRPRHAPLAAWLMLFGAGVGLGYAGHVGMSQLQHYIGGLIADWHLQRAGSDLS
                     RSVTDIGTLGRLKQRDTILARVTGAPRDVVRQRLLHRASYNAYIGTTWLGRRAQSEDL
                     EGEEGGATWTLAEGARVGKIGLAVRVERRRTALPRPPDVTRLTGRAATGVTRNGLGLV
                     QAAVEGDWIQFNAEFGRQIGHYEPPLPEDSALPHGERVAFMRIAEELGLPGLPPEQAV
                     RRVMRHLAGYSYSLWRDAPPPAGVTPLADFMTRTRSGHCEYFAAAAAILLRAAGVPTR
                     YATGYAVMEYSALEESYVVSARHAHAWTRAWVSGRW"
BASE COUNT          939 a         2704 c         2613 g         1066 t
ORIGIN      
        1 ttcaagccgc tcgacctcgg gttcgtcacg ctgccgaacc gcatcctcat gggctcgatg
       61 cataccgggc tcgaggcgcg gcccgacggc atggagcggc tcgcggcgtt ctacgctgag
      121 cgcgcgatgg gcggcgcggc gctgatcgtc accggcggct tctcgcccaa cgacgcgggc
      181 gagctcggcc cgcaccgcgc gcaggtgagc accaaggccg acgccgagcg ccacaaggtt
      241 gtcccgcggg cggtgcacga ggccggcggg cgcatcgcgc tgcaggtgct gcactcgggg
      301 cgctatggct ttcacaagaa caccgttgcg ccctcggcgg tgaagtcggg catcaacccc
      361 aacgcaccgc gcgagatggc cccggacgag atccagaaga cgatcgccgc cttcgcggcg
      421 agcgcggcgc tcgcacgcga agcaggctac gacggcatcg aggtgatggg ctcggagggc
      481 tacctcatct cgcaattcct cgcgccccgc gtcaaccagc gcgcagacga ctggggcggc
      541 agcctggaaa accgcatgcg cttttccgtc gaggtggtgc gcgcctgccg cgtggccgcg
      601 ggcgaggatt tcatcctcct gtaccgcatc tcggcgctcg agctcgttga aggcggcctg
      661 tcggccgagg agatcgccaa ggtggccaag gcgatcgaga aagcgggcgc cacgctcctc
      721 aacaccggca tcggctggca cgaggcgcgc atccccacca tcgcgcaggc cgtgccgcgc
      781 ggcggtttcg cctgggcgac gaggaacctg aagcaggcgg tgaagatccc ggtgatcgcg
      841 tccaatcgca tcaacgcgcc cgaggtcgcc gaggcgatcc tcgcgcgcgg cgacgccgac
      901 atggtgtcgc tcgcgcgggc cttccttgcc gacgaggcgt tcgcggcgaa ggcgaaggcg
      961 ggcgatcgcg ccggcatcaa catctgcatc gcgtgcaacc aggcctgcct cgaccactac
     1021 ttcatcggcc agccggcgag ctgcgtggtc aacccgcgcg ccggccgcga gacgaagctc
     1081 gtgtacgccc ccgcgaagaa aaagaagcgc ctcgccgtgg tgggcggcgg gccggccggg
     1141 ctgtcgtgcg cggcggtcgc ggccgagcgc ggccacgagg tggtgctcta cgacaaggcc
     1201 gccgcgctcg gcggccagtt caatctcgcc aagcgcattc ccggcaagca ggagttcgcc
     1261 gagtcgatcg cctactacgc cgagcgcatc cggcgcgcgg gcgcgaaggt cgtgctcaac
     1321 cacgcgccgg gcgaagccga gctcgcgggc ttcgacgagg tgatcctcgc caccggcatc
     1381 gagccgcgcc gccccgacat cccgggaatc gaccacagga gcgtcgtcgg ctacgtggat
     1441 ctgctttccg gccgcgtcgc gccgggcaag aacgtggtca tcatcggcgc gggcggcatc
     1501 ggcttcgacg tcgcgctcta cctcctggag aagaacgagc gcgccacgct cgagcccgat
     1561 gcgttcgcga agcattgggg catcgtcggc gatcgcgccg ccgcgggcgg tctcgatccg
     1621 aaggggccgc cgcaggcgca tcctgcgcac aagatcacga tgctgaagcg ctccgcgggc
     1681 cccttcggca caacgctcgg gcgcaccacc ggctgggtcc accgcgccga gctcgcgcgc
     1741 aacggcgtcg cgatgctgaa gggcgtcgag taccggcgca tcgacgatgc gggcgtgcac
     1801 attgccatcg acggcaagga acaggtcgtc gccgccgaca cggtggtgat ctgcgccggc
     1861 caggagccgc tgcgcccgct cgcggcgaag cacgtgatcg gcggcgcgaa gctcgccgcc
     1921 gagctcgacg cgaagcgcgc catgctcgag ggcgcggagc tcgccgcggc gctgtagatg
     1981 aacgcggcca cgctcgcggg cggcgccgcg gtgctgctct ggtcgacgct ggcgctcctc
     2041 accgcgggcg cgacgcgcat tccgccgctg cagctcgtct tcctcacctt cgccgtggcg
     2101 agcgcgatcg gcctcgccta tgcgctcctt cggcgcgtga acgtctttcg cgcggcgcgg
     2161 cgccaccccg gcgccctcgc cctctccacc gcggcgctcg gcggctacca cttcttctac
     2221 ttcgtcgcgc tcgccaatgc cccggtggtc gacgcgagcc tcatcgccta cctgtggcca
     2281 ctgctcatcg tcgtgctggc cgccgccgtc ggcggcgcgc gtgtcacgcg ctggcacttt
     2341 ctcggcgccg ttctcggcct cgccggggcg gcgctcgtca tcgcggcgcg cggcgcgctc
     2401 gccgcctcgc cgcagttcct cgccggctac ggcgcggcgc tcgcctgcgc gttcatctgg
     2461 tcgggctact cggtcgccaa ccgccgtcac gccggcgcgc cgaccgagct cgtcgcgctc
     2521 gcctgcgcgc tggtggcgct gctcgcgctt ccggcgcacc tttacttcga gaccttcgcg
     2581 ctgccctcgg accgcgagtg gccctcgatc ctgctgctcg gcgcggggcc cgtgggcgcg
     2641 gcgttctttc tgtgggacta cggcaccaag cacggccgca tcgcgctgct cggcgcgctg
     2701 tcctatgccg cgccggtgct ctccacggcg tttctcgtcg ctgcgggcga cgcggccccc
     2761 acgtggcaca tcggggccgg ctgcgtgctg gtcgcaggcg gtgcggcgct cgccgcttac
     2821 ggcgagcgcc gcgtgggcta acatggccgc catggacaag ccgcccgtcg cagtggtcgc
     2881 cggcgtgggc gaaggcctgg gcttcgccct cggcaagcgc ttcgcgcagg ccggcttcaa
     2941 cgtcgcgctc gcggccagga gcgccgagcg cctcgcgcgc ctggcaggcg aaatccagaa
     3001 agcgggcggc aaggccttcg ccgcgcccac cgatgtgcgc gaggagcagg aggtgatggc
     3061 gctgttcgat gcgctcgagt ccgagcacgg cccggtgcag gtggcggtgt tcaacgcggg
     3121 cgccaacttc cgcgcctcga tcctggagac gcccgccgac atgttcgaga agatctggcg
     3181 cctgggctgc cacgccgggt ttctgttcgg acgcgaggcc gcgcgccgca tgacgccgca
     3241 cggcgcgggc acgattctct tcaccggcgc cacggcgtcg attcgcggtt cgtcgcactt
     3301 cgccgccttc gccgcggcga agaacggcct gcgcgcggta gcgcagtcga tggcgcgcga
     3361 gctcgggccc aagggcatcc acgtcgcgca cgtcgtcatc gacggcatga tcgacacggc
     3421 ggcggtgcgc gcgcgctttc ccgatcgcgt gaaagacctg ggcgtggacg ccctgctccg
     3481 cacggacgcc atcgccgagc tctactacca gatccacgcg cagccgcgct cggcctggac
     3541 cttcgaagcg gacctgcggc ccttcggcga gaagttctag cgtgcaggcg gccgacgcgc
     3601 agcgcctgtt cgagcgcatc gccgccaaca tcgagtcggt gatgcgcggg cagcacgcgc
     3661 aggtgcggaa actcctcgcc gcgttcgccg ccggcggcca cgtgctggtg gacgactttc
     3721 ccggcaccgg caagaccacg ctcgcgaaga gcctcgcggc ctccatcggc gcgccgtttg
     3781 cgcgcgtgca gttcacgccg gatctcttgc cctcggacat cctcggcgtc tctgtcttca
     3841 accagcgcga ccagagcttc gaattccgcg aggggcccgt cttcacctcg atcctgctcg
     3901 ccgacgagat caaccgcgcc tcgccgcgca cccagtcggc gctgctcgag gtgatggccg
     3961 agggccaggt atcggtggaa gggcgcacgc acgccatgga cccgatgttc ttcgtcgtcg
     4021 cgacgcagaa cccggtcgag ttccgcggca cctaccccct gcccgaggcg cagatggacc
     4081 gcttcgcgct gtgcctcgcg ctcggctatg tcgacgccga caccgaggtc gcggtgctct
     4141 cggcgcagga tctcggtcac ccgctcgagc gcatcggccc ggtcgctgcg cgcgaggagg
     4201 tgctggcgct gaaggaagcc gtgcgccgcg tgcgcgtggc cgaggagctg aagcactacg
     4261 tggtggagct ggtgcggcgc acgcgcgccg cgccgcaggt gctgctcggc tgcgggccgc
     4321 gcgcctcgat cgcgctcacg cacacggcga aggccctcgc cctcttcgat ggcgaagaat
     4381 tcgtgcgccc cgagcacgtg caggagctcg ccgtggcgac gctcgcgcat cgcatggtgc
     4441 tcgacccgca ggcccgcttc gccggcgcct cggccgcgtc cctcgtcggc gagcttctgc
     4501 gggaggtgaa agtcccggct tgaggcgccc gatctacctc gcgctgcgag tgttcagcgc
     4561 gctcgatcac tggctgcgcg aacggctcac cgcggccgga tggctcgcgc tcggcgccgc
     4621 gggcgcggcc ggtgccgcgg ggctcgacac cacgcagaac acgagctacc aggcggcgac
     4681 gctcctcggc gcgctgctgc tgcttgcctg ggcggtgagc ctgttctttc gcgcgcgcgt
     4741 cgaggttcgc cgcgagttgc cacgctacgc cactgccgga gagcccttca gctacgtcgt
     4801 cgccgtcgag aaccagggcc cgcgcgccct cgccgggctg acgctgcgcg agcgcctcgc
     4861 cgatccgcgg ccgcgctacg acgagtggcg ccgcgcgcgc gagccgggcg aagagcggcg
     4921 caactggttc gaccgcaacg tgggctactt ccgctggcgc tggctgatcg agcggcgcac
     4981 gccgcagccg ccgcgggaaa gcccgctgcc cgagcttgcg ccgggggcgc ggcgcgcgct
     5041 gcgcctcacg ctcaccccac gccggcgcgg ccgtatcgag ctcgccgggc tcgtgctcgg
     5101 gcgcaccgac ccgctcgggc tggtcagagg cctcgcgccc ctgccgctcg cggcgcacat
     5161 cactgcgctg ccgcgccgct accgcctgcc gcgccttgcg ctccccgggc ggcgccgcca
     5221 ccagcccggc ggcgtgtcgc tcgcgagctc ggtgggcgac tccgaagagt tcctcgcgct
     5281 gcgcgagtac cggccgggcg acccgctgca gcacatccac tggaagagct tcgcgcgcac
     5341 cggccggccg atcgtgaagg agttccagga cgagttcttc gagcgccacg ccctggtgct
     5401 cgatacgggc accgtgcgcg gcgaggattc ggcgttcgag gacgcagtgg cggtggcggc
     5461 gtccttcgtc tactccatcg acacgcagga gtgcctgctc gacctgctct tcgtcggcgg
     5521 cgaggtgcgc cactacgtcg ccgggcgcgg ccagctgcag tccgagcaca tgctcgaggt
     5581 gctcgccggc gtgggcccga gcgcgccgca ggggttcgac gccctctcgc gcgcggtgct
     5641 ggaagcgcgc acgcagctcg caagctgcat cctcgtgctc gtaagctggg acgacgcgcg
     5701 ccggcggctt gccgagtcgc tggcggcgag cggcaccgag gtgcgcgcga tcctcgtctg
     5761 cgcgagcgaa gacaagccgc gcgacgcgcc cgcgtggctc accgcggtgc atcccggcgc
     5821 gatcgaggcg gggctcgcag ggctcgagcg ccgggtgctg cgatgagcct gccgccgttc
     5881 atcgccgggg cggcgctgct cttctggggc tggcacagcg ggaatctcct cgtcgccgcc
     5941 ccgctcgcgc tggccctgga ggcgccgcgc tggctgcggc tgcgctttgc gctcgggcct
     6001 ccagactacg cgcgcatcgc cgacctgtgc acagtgttct tcgtcgggct ggcggtcgcg
     6061 ctggccttcg accgcggcgt ggcacacggc gtcatcggtg ccttccgctg gctgccggtg
     6121 gtcctcgcgc cgattctgct tgcgcagcgc ctaagcgagg acaaccgcgt gccgctcacc
     6181 gcgctgtttc gctacctgcg caagcaaaag cagcgcaatc ccgagctgca ggtaccgctg
     6241 gtggatacgg gcggcgtgtt cgtggtgctg tgcgtggtcg ccgccggcgt cggcaacgcc
     6301 gcaacgcaga cctactatgc cggcgccgtg ctgctcgcgg cctgggcgct ctttgccacg
     6361 cggccgcgcc acgcgcccct cgccgcgtgg ctgatgctgt tcggcgccgg cgtgggcctc
     6421 ggctatgccg gccacgtcgg catgtcgcag ctgcagcact atatcggcgg cctcatcgcc
     6481 gactggcacc tgcagcgcgc gggctccgac ctgtcgcgca gcgtgaccga catcggcacc
     6541 ctggggcgcc tgaagcagcg cgacacgatc ctcgcgcgcg tcaccggcgc gccgcgcgac
     6601 gtggtgcgcc agcgcctgct gcaccgcgcg agctacaacg cctacatcgg cactacctgg
     6661 ctcggccggc gcgcgcagag cgaggatctc gagggcgagg aaggcggcgc gacctggacg
     6721 ctggccgagg gcgcgcgagt cggcaagatc ggcctcgcgg tgcgcgtcga acgccgccgc
     6781 accgcgctcc ctcggccgcc cgacgtgacg cgcctgacgg gccgcgcggc cacgggggtg
     6841 acgcgtaacg gcctcggcct ggtccaggcc gcggtggaag gcgactggat ccagttcaac
     6901 gcggagttcg gacggcagat cggccactat gagccccccc tgcccgaaga cagcgcgctg
     6961 ccgcatggcg agcgcgtcgc gttcatgcgc attgccgagg agctcgggct gcccggcctc
     7021 ccgcccgagc aggccgtgcg ccgggtgatg cgccacctcg cgggctattc gtactcactg
     7081 tggcgcgacg cgccgccgcc ggcgggcgtg acgccgctcg ccgatttcat gacgcgcacc
     7141 aggagcggcc attgcgagta cttcgccgcg gcggcggcga tcctgctgcg cgccgccggg
     7201 gtacccacgc gctacgcgac gggctacgcg gtgatggagt acagcgcgct ggaggagtcg
     7261 tacgtggtga gcgcccgcca cgcgcacgcc tggacgcgcg cctgggtgag cgggcgctgg
     7321 at
//
LOCUS       DBNHKE010000153         5992 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_184838, whole genome shotgun sequence.
ACCESSION   DBNHKE010000153 DBNHKE010000000
VERSION     DBNHKE010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_184838"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="cas6"
                     /locus_tag="AC16O9_10310"
     CDS             <1..639
                     /gene="cas6"
                     /locus_tag="AC16O9_10310"
                     /inference="COORDINATES: protein motif:HMM:NF021100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HIR6517050.1"
                     /translation="VDAAFAIECRSLPVDHAYALSLAVQAALPWYAGEPQAGLHTVHG
                     AASGAGWQRPEGEDAVLQLSHRTRLVLRLPAHRLQDAAALTGQTLDVGGFPMMVGRLT
                     PRPLSRIASLFSRSVIFEGAADEPEFLSAAAEALRALGVATSTMLCGRGVTLATPEGS
                     YRTRSLMLTVATPAQSLLLQREGLGSARKLGCGVFIPHKDVGDLRPAPPEDF"
     gene            670..1011
                     /locus_tag="AC16O9_10315"
     CDS             670..1011
                     /locus_tag="AC16O9_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR6517051.1"
                     /translation="MTYEVNGKTLEATENGYLANADDWDKDVAQAIAAAEGLTLTQDH
                     WDVIEYLRDAWFNHNGEQPNNRAILKAMQDKWAGRKVDNKTLFDLFPGNPSKQAGKVA
                     GLPESMRKGGY"
     gene            1077..1520
                     /locus_tag="AC16O9_10320"
     CDS             1077..1520
                     /locus_tag="AC16O9_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6517052.1"
                     /translation="MSRLLLACCCLLAAFTARAEVVDIDSAELAKLAASGIPVIDIRT
                     TPEWVETGIVPGSHLLTFFDERGRADPAAWLDRVRAIAKPGEPLIVICRTGNRTQPVS
                     QFLSRHAGYPRVYNVRDGIVAWIKERRPIASAAPALAACRAARTC"
     gene            complement(1548..1943)
                     /locus_tag="AC16O9_10325"
     CDS             complement(1548..1943)
                     /locus_tag="AC16O9_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517053.1"
                     /translation="MFDPITLPFGAKMLFNTVKLKPGVKFEDVELAVGEMCNVVKETY
                     GGDKGGFIAGQVFKFSGFVSDEGSLNATREAEEHYVIVTYWRSFEEHERSHADATFTN
                     RFEALAKMCAETKELGYDMLWQGEPEGQG"
     gene            2012..2353
                     /locus_tag="AC16O9_10330"
     CDS             2012..2353
                     /locus_tag="AC16O9_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR6517054.1"
                     /translation="MAYDVNGKVLETTDNGYLANQDDWDKDVAQAIAAAEGLTLTQDH
                     WDVIEYLRDAYFNRNGEQPNNRAILKAMQEKWPERKVDNKTLFDLFPGNPSKQAGMIA
                     GLPESMRKGGY"
     gene            complement(2397..2537)
                     /locus_tag="AC16O9_10335"
     CDS             complement(2397..2537)
                     /locus_tag="AC16O9_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517055.1"
                     /translation="MNSSPGKALVRWAIVAAATLVFFYALDHALMSAQGLPLNWNMSP
                     RG"
     gene            complement(2559..3290)
                     /locus_tag="AC16O9_10340"
     CDS             complement(2559..3290)
                     /locus_tag="AC16O9_10340"
                     /inference="COORDINATES: protein motif:HMM:NF026037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR6517056.1"
                     /translation="MKGSYGFTLALGCLLGFAGAAAAADYKVVSGGVKGGGTVSGRVS
                     LSGPAPAPATLKVDEDKEACGGDRPSDELLVGANSGVQNVVVEIEGIGAGKDWDFPET
                     FTYDQKKCMFAPRIMLIKPNMPGEVLNSDTIGHNFHTISKGVYNINKKIQPGSKLVVN
                     KNNIRRSGLVRAKCDIHSWMGGAWYVAETPYTVLTDKDGNFTLKDVPPGSYTLKVWHE
                     KLGESEQKIVVKGGETAKADVSMKP"
     gene            complement(3368..5809)
                     /locus_tag="AC16O9_10345"
     CDS             complement(3368..5809)
                     /locus_tag="AC16O9_10345"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6517057.1"
                     /translation="MKLRLPAFWQAVLVLIGAWLVFSLAVPPFMPRSVMVAYMIIVSV
                     GVILYFSSDEDRWREFKAPIAATLRNDNTWPVRWALVIALPVIPGYLVYDALKPSRET
                     PLELRQVHPSPPASIRVYGKSYDLAKLENPVRMEVLKQLETNADAAWQTYREAVQAGS
                     QTYYQNCFYCHGDLLNGKGHFARGLDPLPTDFSDVGTIAQLQESFLFWRITTGGPGLP
                     EGGMPWNSAMPVWHEMLSEEEVWNVITFLFDYVDQVPRMWDPGISKAVTTMKDQVAAQ
                     RAKMDSKAIYQLRCAVCHGEEGAGDGPAAEFLSPRPRDFTGGLWKYKSSPGDLPPRDA
                     DLAETIRDGLHGTSMPGWSSVLTEQQIAGLVALLKRFDLSGFWAPKDAKNEDFDSEGR
                     YTKPNARVITEVEPTDGQVAYSPESVAKGKPVFEENCRKCHGEAGRGDLTSGKLLDDD
                     WGERVWPRDLTKPWTWRATQAATEAELLERKDARRDETIRNIYRRVSIGIRGTPMPAH
                     RATQEGEEDAITPEDRWHVANYVYSLREGATGPGERKVLEALEVAGALPGSAEDAAWS
                     KALPSTFRLVPNIVKDGRLFTPLNDAITVRALYNEREIAFLLEVNDRTESRPGGDVVS
                     QLPRKSEKFFPDAVAIQFPKEGAYSTAPVEKPLYRHGDPKHPTTIWYWNAGAVEPLVE
                     PSAALFDASGPDAKLVRRTEAQDLSASGQWTQGRWRVVMKRPRGDLAQPPASGTDVVF
                     RKGQFIPVSFANWDGNNGEVGSKHTLTPWFWLLLPPETNYLRIYGYALGTVLAFLLAG
                     VVLVWSERRRRPARA"
     gene            complement(5806..>5992)
                     /locus_tag="AC16O9_10350"
     CDS             complement(5806..>5992)
                     /locus_tag="AC16O9_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HIR6517058.1"
                     /translation="GPSLADVGKRLSVEQIRTSIVDPKAVIAQGFPPIMPEFPNMTLT
                     ELDLLVRFLAQQKGAVK"
BASE COUNT          945 a         2017 c         1995 g         1035 t
ORIGIN      
        1 gtggacgccg cgttcgcgat cgagtgccgc agcctgccgg tggaccatgc ttatgcgctt
       61 tcgctggcag tgcaggcggc gctgccgtgg tacgccgggg agccgcaagc gggtttgcat
      121 accgtgcacg gcgcggcctc gggcgcgggc tggcagcggc ccgagggcga ggacgcggtt
      181 ctgcagcttt cccaccgcac gcggctcgtg ctgcggctgc ccgctcaccg cttgcaggac
      241 gcagcggcgc tcaccggcca gaccctggac gtcggcggct ttccgatgat ggtgggccgg
      301 ctgacgccgc ggccgctctc gcgcatcgcg tcgctgttct cgcgcagcgt gatcttcgaa
      361 ggcgcggccg acgagccgga atttctatct gccgcggccg aggccctgcg cgcgctcggc
      421 gtggcgactt cgaccatgct ctgcgggcgc ggggtgacgc tcgccacgcc ggaaggcagt
      481 tatcggacgc gcagcctgat gctgaccgtc gccacgcccg cgcagtcgtt gctgcttcag
      541 cgggagggac tgggctcggc gcgcaagctc ggctgcggcg tcttcattcc ccacaaggac
      601 gtgggcgatt tgcggcccgc gccgccggaa gatttctgac aacttgatcc ggcctcagga
      661 gacaggacca tgacgtacga agtgaacggc aagacgcttg aagcgactga gaacggctac
      721 ctggcgaacg ccgacgactg ggacaaggac gtcgcgcagg cgatcgccgc cgcggaaggg
      781 ctcacgctca cccaggatca ctgggacgtg atcgagtacc tgcgcgatgc ctggttcaac
      841 cacaacggcg agcagcccaa caaccgcgcc atcctcaagg cgatgcagga caagtgggcc
      901 gggcgcaagg tggacaacaa gacgctgttc gacctcttcc cgggaaatcc gtccaagcag
      961 gcggggaagg tcgcggggct gcccgagagc atgcgcaagg gcggctactg acagagtcaa
     1021 atacaatcag cccgatacgg gaccctcttt gcatcggtag cccggagaag gatttcatga
     1081 gccgcctgct tctcgcctgc tgctgcctcc tcgccgcctt cactgcccgc gccgaggtcg
     1141 tcgatatcga tagtgccgag ctggcgaagc tggcggcttc gggcattccg gtgatcgaca
     1201 tccgcaccac gccggagtgg gtggaaaccg gcatcgtccc cggcagccac ttgctgactt
     1261 tcttcgacga gcgcggcagg gctgatcccg ccgcgtggct ggatcgggtc agagccatcg
     1321 ccaagcccgg cgagccgctg atcgtcatct gtcgcaccgg caatcgcacc cagcccgtca
     1381 gccagttcct gtcgcggcac gcgggatacc ccagggtcta caacgtcagg gacgggatcg
     1441 tcgcctggat caaggagcgc cggccgatcg cgtctgccgc gccggcgctg gccgcctgcc
     1501 gcgcggccag gacttgctga agctgcttgc gcgcgggagg ggtgggacta gccttggcct
     1561 tccggctcgc cctgccagag catgtcgtaa ccgagctcct tggtctcggc gcacatcttc
     1621 gccagcgcct cgaacctgtt ggtgaaggtc gcgtcggcgt gcgagcgctc gtgctcctcg
     1681 aaggagcgcc agtaggtgac gatcacgtag tgctcctcgg cctcgcgcgt ggcgttgagc
     1741 gagccctcgt cggagacgaa gccggagaac ttgaacacct gtccggcgat gaagccgccc
     1801 ttgtcgccgc cgtaggtctc cttgacgacg ttgcacatct cgcccaccgc cagctcgacg
     1861 tcctcgaatt tgacgccggg cttgagcttg acggtgttga acagcatctt cgcgccgaag
     1921 ggcagcgtga ttggatcgaa catcggcggg ctccgtaggt aagaaggttt ttcgacaacc
     1981 ccattttgcc ccggtttcag gagaaaggac gatggcgtac gacgtgaacg gaaaggtgtt
     2041 ggagacgacc gacaacggct acctggcgaa ccaggacgac tgggacaagg acgtcgcgca
     2101 agcgatcgcc gccgccgagg ggctgaccct cacccaggac cattgggacg tgatcgagta
     2161 cctgcgcgac gcctacttca accgcaacgg cgagcagccg aataaccgcg cgatcctcaa
     2221 ggccatgcag gaaaagtggc ccgagcgcaa ggtcgacaac aagaccctgt tcgatctctt
     2281 cccggggaac ccctccaagc aggcgggcat gatcgccggg ctgcccgaga gcatgcgaaa
     2341 gggcgggtac tgagcccgcc cgggcaggaa aggcgcctgc aatccgccgc gcggcgctag
     2401 ccgcgcggcg acatgttcca gttgagcggc agcccctgcg cgctcatcag ggcgtggtcc
     2461 agggcgtaga agaacaccag ggtcgcggcc gccacgatcg cccagcgcac aagcgctttc
     2521 ccgggactcg agttcacgcc ctcgctccct cgttcgccct acggcttcat cgagacgtcg
     2581 gccttcgccg tctcgccgcc cttgacgacg attttctgct cggactcgcc cagcttttcg
     2641 tgccagacct tgagggtgta gctgcccggc ggcacgtcct tcagggtgaa attgccgtcc
     2701 ttgtccgtga gcaccgtgta cggcgtttcc gccacgtacc acgccccgcc catccacgag
     2761 tggatgtcgc acttggcgcg caccaggccc gagcgccgga tgttgttctt gttcacgacc
     2821 agcttgctgc cgggctggat cttcttgttg atgttgtaga cccctttgct gatcgtgtgg
     2881 aagttgtggc cgatcgtgtc gctgttcagc acctcgcccg gcatgttggg cttgatgagc
     2941 atgatgcgcg gcgcgaacat gcactttttc tggtcgtagg tgaaggtctc ggggaagtcc
     3001 cagtccttgc cggcgccgat gccctcgatc tcgacgacga cgttctgcac cccgctgttc
     3061 gcgccaacga gaagctcgtc ggagggccga tccccgccgc aggcttcctt gtcctcgtcg
     3121 accttgaggg tcgccggcgc gggtgcgggg ccgctcagcg acacgcgccc gctcacggta
     3181 ccgccgcctt tcacgccgcc gctcacgacc ttgtagtcgg cggcggcagc cgcgcccgcg
     3241 aaccccagca ggcagcccag agccagagtg aatccgtacg atcctttcat gtatgttctc
     3301 ccggttgtac gcagatagtt agcatcgagc agtgacacct tggtgacatg ggggcgcagg
     3361 cccggggtca cgcgcgcgcc gggcgcctgc ggcgctcgct ccacacgagg acgactccgg
     3421 cgagcaggaa cgcgagcacg gtgcccagcg catagccgta gatccgcagg tagttcgtct
     3481 ccggcggcag cagcagccag aaccacggcg tcagcgtgtg cttggacccg acctcgccgt
     3541 tgttgccgtc ccagttggcg aacgacaccg ggatgaactg acccttgcgg aaaacgacgt
     3601 cggtgccgct tgccggcggc tgggcgaggt cgccgcgcgg gcgcttcatg accacccgcc
     3661 agcgcccttg cgtccattgc ccgcttgcgc tgaggtcctg ggcctcagtg cgccgcacga
     3721 gcttcgcatc cgggccgctg gcgtcgaaga gcgccgcgct cggctcgacc aggggctcga
     3781 cggctcccgc gttccagtac cagatggtgg tgggatgctt cgggtcgcca tgtcggtaga
     3841 gcggcttttc caccggcgcg gtgctgtagg cgccctcctt ggggaactgg atcgccacgg
     3901 catccgggaa gaacttctcg ctcttgcgcg gaagctgcga gaccacatcg cccccggggc
     3961 ggctttcggt gcgatcgttc acctccagga gaaaggcgat ttcccgctcg ttgtagagcg
     4021 cgcgcaccgt gatggcgtcg ttcagtggcg tgaacagccg cccgtccttc acgatgttcg
     4081 gcaccagccg gaaagtgctc ggcagggcct tgctccaggc cgcgtcttct gcgctgcccg
     4141 gcagcgcgcc cgccacttcg agcgcctcca gaaccttgcg ctcccccggc cccgtggccc
     4201 cctcgcgcag cgagtagacg tagttggcga cgtgccagcg gtcttcgggc gtgatcgcgt
     4261 cctcctcgcc ttcctgggtg gcgcggtgcg cgggcatggg cgtgccgcgg atgccgatcg
     4321 acacgcgcct gtagatgttg cggatggtct cgtcgcgacg cgcgtccttg cgctccagca
     4381 gctcggcctc ggtcgcggct tgcgtggcgc gccaggtcca cggcttggtg aggtcgcgcg
     4441 gccagacgcg ctcgccccag tcatcgtcga gcagcttgcc cgaggtgagg tcgccgcgcc
     4501 ccgcctcgcc gtggcacttg cggcaattct cctcgaacac cggcttgccc tttgccaccg
     4561 actcggggga gtaggcgacc tggccgtccg tgggctcgac ctccgtgatc acccgcgcgt
     4621 tgggctttgt gtagcgccct tccgagtcga agtcctcgtt cttcgcatcc ttcggcgccc
     4681 agaagcccga gaggtcaaag cgcttgagca gcgccacgag gccggcgatc tgctgctcgg
     4741 tgagcaccga ggaccagccc ggcatgctgg tgccgtgcag gccgtcgcgg atcgtctcgg
     4801 cgaggtccgc atcgcgcggg ggcaggtcgc cgggcgagct cttgtacttc cacaggccgc
     4861 cggtgaagtc gcgcggccgc ggcgaaagga actccgccgc cggcccgtcg cccgcgcctt
     4921 cttcgccgtg gcagacggcg cagcgcaact ggtagatcgc cttgctgtcc atcttggccc
     4981 gttgcgccgc aacctgatcc ttcatggtcg tgaccgcctt ggagatgccg ggatcccaca
     5041 tgcgcggcac ctggtcgacg taatcgaaga ggaaggtgat gacgttccac acctcttcct
     5101 cggacagcat ttcgtgccag accggcattg ccgagttcca gggcatgccg ccctccggca
     5161 gcccggggcc gcctgtcgtg atgcgccaga acaagaacga ttcctgcagc tgcgcgatcg
     5221 tgccgacgtc gctgaagtcg gtgggcagcg gatccaggcc cctcgcgaag tggcctttgc
     5281 cgttcagcaa atcgccgtga cagtaaaagc agttctggta gtaggtctgg ctgcccgcct
     5341 gcaccgcctc gcgataggtc tgccacgccg catccgcatt ggtctcgagc tgcttgagca
     5401 cctccatgcg caccgggttt tcgagcttgg cgaggtcgta ggacttgccg tagacccgga
     5461 tgctcgcggg cggcgacggg tgcacctggc gcagctcgag cggcgtctcg cgcgagggct
     5521 tgagcgcgtc gtagaccagg taacccggga tgaccggcag cgcgatcacc agggcccagc
     5581 gcacgggcca ggtattgtcg ttgcgcaggg tcgcggcgat cggcgccttg aactcgcgcc
     5641 agcggtcctc gtcgctggag aagtacagga tcacgccgac ggacacgatg atcatgtagg
     5701 cgaccatcac ggagcgcggc atgaacggcg gcactgcgag gctgaacacg agccaggcgc
     5761 cgatgagcac cagcacggcc tgccagaagg ccggcagacg cagcttcact tcaccgcccc
     5821 cttctgctgc gcgaggaaac gcaccagcag atcgagctcc gtcagcgtca tgttcgggaa
     5881 ctccggcatg atcggcggaa agccctgggc gatgacagcc ttgggatcga cgatgctcgt
     5941 gcgaatctgc tcgacgctca ggcgcttgcc cacgtccgcg aggctggggc cg
//
LOCUS       DBNHKE010000154         7210 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_502636, whole genome shotgun sequence.
ACCESSION   DBNHKE010000154 DBNHKE010000000
VERSION     DBNHKE010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_502636"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..120)
                     /locus_tag="AC16O9_10355"
     tRNA            complement(44..120)
                     /locus_tag="AC16O9_10355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Arg,seq:acg)
     gene            complement(148..684)
                     /locus_tag="AC16O9_10360"
     CDS             complement(148..684)
                     /locus_tag="AC16O9_10360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6517059.1"
                     /translation="MDQPVNADQFKLGMRGLAHAVNVITAAHAGHRYGLTATAVCSVT
                     AEPPTLLVCVNQRAATHGALAKSKAFCVNVLRAEDSDLSNLFSGAQSGEKRFDTREWT
                     KLATGSPVLLDALVSFDCRVVKKLKHGSHTVFLGQVQQVLFGKKGKPLLYAEGQYAKL
                     ASLAHGEPLPEGLDYWGF"
     gene            735..2522
                     /locus_tag="AC16O9_10365"
     CDS             735..2522
                     /locus_tag="AC16O9_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HIR6517060.1"
                     /translation="MAEHGFLYQALIYLAAGVIAVPLFTKLGLGSVLGFLAAGMVIGP
                     WGLKLVSKPEAVLHFAEFGVVLLLFLVGLELNPQRLWQMRRPIFGMGGTQVVATLLLV
                     TAIGWALDQPLPIAVVAGMGFAMSSTAIALATLQERRLLGTPGGQAGFSVLLFQDLAV
                     IPLALLLALLAPGASSGGMDWMAAARAGVVIAALIAVGHFGLRPLLRVVANTGQREIF
                     IGFALLLVLGIAALMESLGLSMALGSFLAGVMLAESEYRHELELDIEPFKGLLLGLFF
                     IAVGMSIDLNLFLRTPLLILGLALGVVALKALLLYPIARYFGYCAPSDAFVFALSLSQ
                     VGEFAFVLFAAAGSILPRETYNVLNAVVAVSMVATPFLMILYERGIAPRLARGAEREA
                     DVVDEALPVIVAGFGRFGQVVTRVLNGLGVHATLIDHDPNQIELVRRFGSKAYYGNAT
                     RLDVLEKAGVARARLLVVAIDDNVAALRLTREVRKRYPGLKVIARAHGRSDAFEYLDL
                     GVPAVRETFGSALDAAEAALRALDFGPMAARRIVTRFRRHDEEILAEQAPHRGEIKQL
                     IAMQQRGRDDLERLLRTETEAVARKPSAG"
     gene            complement(2509..3393)
                     /locus_tag="AC16O9_10370"
     CDS             complement(2509..3393)
                     /locus_tag="AC16O9_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6517061.1"
                     /translation="MDHRKPLDAQAFFLMVLLTALWGFQQVAIKLTAPDVSLVMQAAL
                     RTLLAAALLLAWARWRAIPLFSRDGTLAAGIGAGLLFGGEFVFIYAGLEYTAASRMVV
                     FIYLAPIVTALGVHWWVPGEHLVRTQWVGVALAFGGVALAFGDGFSAAQGATLLGDAC
                     GVVAAVLWGATTVLIRTTSLSRATAAKTLFYQLAVAVPLLFGASLLLGEPGVVALSPF
                     ALASLAYQGAIVAFASYLAWFWLLTRYLAGRLAVFSFMTPLFGVLAGVAVLGERLTPT
                     FAAAALLVGAGIVLVNRR"
     gene            3501..4238
                     /locus_tag="AC16O9_10375"
     CDS             3501..4238
                     /locus_tag="AC16O9_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR6517062.1"
                     /translation="MFPNREGQRVPQVAFRARANGEWQTVTTDEIFKGRTVVVFSLPG
                     AFTPTCSSTHLPRYNELAPAFRASGVDDIVCVSVNDGFVMSEWAKDQEAANVRLIPDG
                     NGEFTEKMGMLVDKADLGFGKRSWRYSMLVRDGVIEKMFVEPQKPGDPFEVSDADTML
                     AYINPSAKKPDQVAILTREGCGFCAKAKALLTEMGYDYAEIPLDHKVRTRVVGAISGA
                     QTVPQVFINGVRIGGWDTLEQWAKKAA"
     gene            complement(4239..5033)
                     /locus_tag="AC16O9_10380"
     CDS             complement(4239..5033)
                     /locus_tag="AC16O9_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6517063.1"
                     /translation="MKTTYATLALKEEGAHVLVVTLNRPEVLNAISTQMGRDWLELFN
                     GLAADPGELRCIVLTGAGERAFCAGADLKERDGMSVETWRTQHAQFEQAFVALMECPV
                     PIIAAVNGHAYGGGLETALCCDFIYAARGAKLALSELRLGIMPGGCGTQNLPRAVGER
                     RAKELILTARAFTAEQGAEWGLVNKVCEPGKTLADALETAQAIAANAPIAVRQAKKSI
                     HYGLQMDLLTGYRFEIEAYNRLVDTEDRREGVRAFNEKRAPAFKGR"
     gene            5141..6325
                     /locus_tag="AC16O9_10385"
     CDS             5141..6325
                     /locus_tag="AC16O9_10385"
                     /inference="COORDINATES: protein motif:HMM:NF016103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HIR6517064.1"
                     /translation="MIRCLLLPPLLALAAAAAAHEAPAPAARAECVPVAAWVAPGGEK
                     LAARDVLSRAAEASVVLLGESHESAEHHRWQLQTVAALHAQRPDLVLGVEAFPRRVQP
                     VLERWVAGELSEKEFLERSDWRSVWRVDAELYLPLFHFARMQRIPMLALNVEQSLTRE
                     ITRKGYDAVPAERREGVGRPAPASEAYIDWLLPVWGEHERPGGKPAKLERDDPDFRRF
                     VESQTTWDRAMAEALAAAARRPGAPLVIGIMGTGHVAHGYGVPHQLKALGIANLTTLL
                     PWDRGAECKNFVAGFADAVFGLAEPADARAQARQRLGVYLEAASEGVRILKVEKGSLA
                     EAAGVRDGDLLSEIAGVTPKQVGDVVEVVQRQAPGTWLPLKVRRGEATLELVAKFPPR
                     AS"
     gene            6322..>7210
                     /locus_tag="AC16O9_10390"
     CDS             6322..>7210
                     /locus_tag="AC16O9_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 aminopeptidase family protein"
                     /protein_id="HIR6517065.1"
                     /translation="MKARARALALALAAAPALAGAAQLELEAALDPAHGTLRARAVLV
                     VGRAAAEISLAARFAVDAVALDGRRGELAGTLRDGLHVWRLPAADRERRVEIAWHGAL
                     DPLDETLLHRDTLGQPRPVAGARGSFLPGAARWHPALGEALEGYRVTIEVPEGQRALV
                     AGRLLEERVSGARYRARFEYPHPAQDIDLIAGPYRIEDRAVRTAAGSAVRLRTYFHPE
                     IAPLGPGYLDAVLGYLDRYEAWIGAYPHTEFSVVSSPTPTGFGMPSLTYLGIDVLRLP
                     FIRFTSLGHEVLHNWWGNGV"
BASE COUNT         1079 a         2535 c         2499 g         1097 t
ORIGIN      
        1 gcgtcgaaac gaaaagggcc agcgcttgct ggccctttgt atttggcgcg cccggcagga
       61 tttgaaccca cgaccccttg gttcgtagcc aagtactcta tccaactgag ctacgggcgc
      121 gaggccgcag attatagcgg aaagccctca gaacccccag taatccagcc cctcgggcag
      181 cggctcgccg tgcgcgagcg acgccagctt ggcgtactgg ccttcggcgt acagcaaagg
      241 cttgcccttc ttgccgaaca gcacctgctg cacctgcccg agaaagacgg tatgcgagcc
      301 gtgcttgagc ttcttcacca cgcggcagtc gaacgacacc agcgcatcga gcagcaccgg
      361 cgagccggtg gcgagcttgg tccattcgcg cgtgtcgaac cgcttctcgc ccgactgcgc
      421 gccgctgaac aggttggaca gatccgaatc ctccgcgcgc agcacgttca cgcagaacgc
      481 cttgctcttg gccagcgcgc cgtgcgtcgc cgcgcgctgg ttcacgcaca ccaatagcgt
      541 cggcggctcg gcggtcaccg agcacaccgc cgtcgcggtg aggccgtagc gatgcccggc
      601 gtgcgctgcg gtgatcacgt tcaccgcgtg cgccaggccg cgcatgccga gcttgaactg
      661 gtcggcgttg accggctggt ccatgcacgc gattattgca cgcacccccc gcgcatcttt
      721 ccacggagca gcctatggct gagcatggct ttctgtacca ggcgctgatc tatctcgccg
      781 ccggcgtgat cgcggtgccg ctgttcacga agctgggcct cggctcggtg ctcggcttcc
      841 tcgccgccgg catggtgatc ggcccctggg gcctgaagct cgtctcgaag cccgaggcgg
      901 tgctgcattt cgccgagttc ggcgtggtgc tgcttctctt tctcgtcggg ctcgagctca
      961 acccgcagcg cctgtggcag atgcgccgcc cgatcttcgg catgggcggg acgcaggtcg
     1021 tcgccaccct cctgctggtg accgcgatcg gctgggcgct cgatcagccg ctccccattg
     1081 cggtcgtcgc cggcatgggc ttcgccatgt cctccaccgc gatcgccctt gccacgctgc
     1141 aggagcggcg cctgctcggc acgcccggcg ggcaggccgg cttctcggtg ctgctgttcc
     1201 aggacctggc cgtgatcccg ctcgcgctgc tgctggcgct gctcgcgccg ggcgcgtcga
     1261 gcggcggcat ggactggatg gccgccgcgc gggcgggggt ggtcatcgcg gcgctcatcg
     1321 ccgtcggcca tttcggcctg cggccgctgc tgcgcgtggt cgccaacacc ggccagcgcg
     1381 agatcttcat cggctttgcg ctgctcctcg tgctcggcat cgcggcgctg atggagagcc
     1441 tcggactgtc gatggcgctc ggttcgttcc tcgccggcgt catgctggcg gagtccgaat
     1501 accgccacga gctcgaactc gacatcgagc cgttcaaggg gctgctgctc ggcctgttct
     1561 tcatcgccgt cggcatgtcg atcgacctga acctgttcct gcgcacgccg cttctcatcc
     1621 tcgggctcgc gctcggcgtc gtggcgctga aggcgctgct gctctacccg atcgcgcgct
     1681 acttcggcta ctgcgcgccg tccgacgcgt tcgtgttcgc gctgagcctg tcgcaggtgg
     1741 gcgagttcgc gttcgtgctg ttcgccgccg ccggcagcat cctgccgcgc gagacctaca
     1801 acgtgctgaa cgccgtcgtg gccgtgtcca tggtggcaac gccgttcctc atgatcctgt
     1861 acgagcgcgg gatcgccccg cgcctcgccc gcggcgccga gcgcgaggcc gacgtggtcg
     1921 acgaggcgct tcccgtgatc gtcgccggct tcggtcgctt cggccaggtg gtgacgcgcg
     1981 tgctgaacgg cctcggcgtg cacgccaccc tcatcgacca cgacccgaac cagatcgagc
     2041 tggtgcgccg cttcggctcc aaggcctact acggcaacgc cacgcgcctc gacgtgctgg
     2101 agaaggcggg cgtggcgcgc gcacggctgc tggtggtcgc gatcgacgac aacgtcgccg
     2161 cgctgcgcct cacgcgcgag gtgcgtaagc gctatcccgg gctcaaggtc atcgcgcgcg
     2221 cgcacggccg cagcgacgcc ttcgagtacc tcgacctggg cgtgccggcg gtgcgcgaga
     2281 cgttcggctc ggcgctggat gccgcggagg ctgcgctgcg cgcgctcgac ttcggcccca
     2341 tggcggcgcg gcgcatcgtg acgcgcttcc ggcgccacga cgaggagata ctcgccgagc
     2401 aggcaccgca tcgcggcgag atcaagcagc tgatcgcgat gcagcagcgc ggccgcgacg
     2461 acctcgagcg gctgctgcgc accgagaccg aggccgtcgc gcgcaagcct agcgccggtt
     2521 gaccagcacg atgccggcgc cgacgagcag cgccgcggcg gcgaaggtcg gcgtgagccg
     2581 ttcgccgagc acggccaccc cggcaagcac gccgaacagc ggcgtcataa acgagaacac
     2641 cgccagccgc cccgccaggt agcgggtgag cagccagaac cacgcaaggt agctggcaaa
     2701 ggcgacgatc gcaccctgat acgcgaggct cgcgagcgcg aagggcgaga gcgcgacgac
     2761 gccgggctcg ccgagcagca gcgaagcgcc gaacagcaac ggcacggcca ccgcaagctg
     2821 gtagaacagc gtcttcgccg ccgtggcgcg ggaaagcgag gtggtgcgga tcagcaccgt
     2881 cgtcgccccc cacagcacgg cggccaccac gccgcaggcg tccccaagaa gcgtcgcgcc
     2941 ctgcgcagcg ctgaagccgt caccaaaggc gagcgccacg ccgccgaaag cgagcgccac
     3001 gcccacccat tgcgtacgca cgaggtgctc gccgggcacc caccagtgca cgccgagggc
     3061 ggtgacgatg ggcgcgaggt agatgaacac caccatgcgc gaggccgcgg tgtactcgag
     3121 gccggcgtag atgaagacga actcgccgcc gaacagcaac ccggcgccga taccggccgc
     3181 cagcgtgccg tcgcgggaaa acagcggaat ggcgcgccag cgcgcccagg cgaggagcag
     3241 cgccgcggcg agaagcgttc gcagcgccgc ctgcatcacg agggacacgt ccggcgccgt
     3301 cagcttgatc gccacctgct ggaatcccca caatgccgtg agcagcacca tgaggaaaaa
     3361 cgcctgcgca tccaaaggct tgcggtgatc catggaaatt agctatggcc tatgggcgcg
     3421 atgcggacaa gtcattggat cgggtggcaa agcgcccgta cagtcgtagc cttagtcaat
     3481 cactaacgag gacttgatag atgtttccca accgcgaagg acagagggta ccccaggttg
     3541 ctttccgcgc gcgcgccaac ggcgagtggc aaaccgtgac caccgacgag atcttcaagg
     3601 gccgcacggt ggtcgtgttc tcgctgccgg gcgccttcac cccgacctgc tcgtccacgc
     3661 acctgccgcg ctacaacgag ctcgcgcccg ccttccgcgc cagtggcgtg gacgatatcg
     3721 tgtgcgtgtc cgtgaacgac ggcttcgtga tgagcgagtg ggcgaaggac caggaagccg
     3781 ccaacgtgcg gctgatcccc gacggcaacg gcgaattcac tgaaaagatg ggcatgctcg
     3841 tcgacaaggc cgacctcggc ttcggcaagc gctcctggcg ctattcgatg ctggtgaggg
     3901 acggcgtgat cgagaagatg ttcgtcgagc cgcagaagcc gggcgacccg ttcgaagtgt
     3961 ccgacgccga cacgatgctc gcctacatca atccgtcggc gaaaaagccc gaccaggtgg
     4021 cgatcctcac gcgcgagggc tgcggcttct gcgcgaaggc gaaggcgctg ctcaccgaga
     4081 tgggctacga ctacgccgag atcccgctcg accacaaggt gcgcacgcgg gtggtgggcg
     4141 cgatctccgg cgctcagacg gtgccgcagg tgttcatcaa cggcgtgagg atcggcggct
     4201 gggacaccct cgagcagtgg gcgaagaagg cggcgtaatc agcgcccctt gaacgcgggc
     4261 gcgcgcttct cattgaaggc gcgcacgcct tcccttcggt cctccgtatc gacgaggcgg
     4321 ttgtaagcct cgatctcgaa ccgatagccg gtgagcaaat ccatctgcag cccgtagtgg
     4381 atggacttct tcgcctggcg caccgcgatg ggcgcgttcg ccgcgatcgc ctgcgccgtc
     4441 tccagggcgt ccgccagggt cttccccggc tcgcacacct tgttgacgag cccccattcg
     4501 gcgccctgct ccgcggtgaa ggcacgcgcg gtcaggatca gctccttcgc gcggcgctcg
     4561 cccaccgcgc gcggcaggtt ctgcgtgccg cagccccccg gcatgatgcc caggcgcagc
     4621 tccgacaacg ccagcttcgc ccccctggcc gcgtagatga aatcgcagca cagggccgtc
     4681 tccagcccgc cgccgtaggc atgcccgttc accgcggcga tgatcggcac cgggcactcc
     4741 atcagcgcga cgaacgcctg ctcgaactgc gcgtgctgcg tgcgccaggt ctcgaccgac
     4801 atgccgtcgc gctccttgag gtcggcgccg gcgcagaacg cgcgctcgcc cgcgccggtg
     4861 agcacgatgc agcgcagttc accggggtcg gcggcgaggc cgttgaacag ctcgagccag
     4921 tcgcgcccca tctgcgtgct gatcgcgttc agcacctcgg ggcggttgag cgtcacgaca
     4981 aggacgtgcg cgccctcctc tttcagggcc agcgtggcgt aggtcgtttt catcggggtc
     5041 cttggttgga atcggcgaaa ggggcatcta taccgttcct gtccaacgca gtccagcggc
     5101 gaactggcgt aatcttgggc atgcgcaagg tgcctgtctc atgatccgct gcctattgct
     5161 tccgccgctg ctcgccctgg cggctgcggc cgccgcgcac gaagcgccgg cgcccgcggc
     5221 gcgcgccgag tgcgtgcccg tggcggcgtg ggtcgcgccg ggaggcgaaa agctcgctgc
     5281 gcgcgacgtg ctgagccggg cggccgaggc gtccgtggtg ctgctcggcg aatcgcacga
     5341 aagcgcggag catcatcgct ggcagctgca gaccgtcgcc gcgctgcacg cgcagcgacc
     5401 ggacctggtg cttggcgtcg aggccttccc gcgccgggtg cagccggtgc tcgagcgctg
     5461 ggtggccggc gagctctcgg aaaaggagtt cctcgagcgc tccgactggc gcagcgtgtg
     5521 gcgcgtggac gccgagctct acctgccgct gtttcatttc gcgcgcatgc agcgcatccc
     5581 gatgctggcg ctgaacgtgg agcagtcgct gacgcgggag atcacacgca agggctacga
     5641 cgcggtgccc gcggagcgcc gcgaaggcgt agggcggccc gcgccggcga gcgaagccta
     5701 catcgactgg ttgctgccgg tgtggggcga gcacgagcgg cccggcggca agcccgccaa
     5761 gctggagcgc gacgacccgg actttcgccg cttcgtggag agccagacca cgtgggaccg
     5821 cgcgatggcc gaggcgcttg cggcggccgc gcgtcgcccg ggcgcaccgc tggtgatcgg
     5881 catcatgggc acggggcatg tcgcccacgg ctacggcgtg ccgcaccagc tcaaggcact
     5941 gggcatcgcc aatctcacga cgctcctgcc gtgggatcgc ggcgccgagt gcaagaactt
     6001 cgtcgccggg ttcgccgacg cggtcttcgg cctcgccgag ccggccgatg cgcgcgcgca
     6061 ggcgcggcag cggctcggcg tctacctgga ggcagcgagc gagggcgtgc gcatcctcaa
     6121 ggtggaaaag ggcagcctcg ccgaggcggc gggcgtgcgc gacggcgact tgctcagcga
     6181 gatcgcgggc gtgacgccca agcaggtcgg cgacgtggtc gaggtggtgc agcgccaggc
     6241 gcccggcacc tggctgccgc tcaaggtgcg ccgcggcgag gcgacgctcg agctggtcgc
     6301 caagttcccg ccgcgcgcct catgaaggcg cgcgcaaggg cgctcgcgct ggcgctcgcg
     6361 gcggcgccgg cactcgcggg tgccgcgcag ctcgagctcg aggcggcgct cgatcccgcg
     6421 cacggcacgc tgcgcgcgcg tgccgtgctc gtcgtcggcc gcgcggccgc cgaaatctcg
     6481 ctcgctgcgc gcttcgcggt ggatgccgtc gcgctcgacg gccggcgagg cgaactggcc
     6541 ggcacgctgc gcgacggcct gcatgtgtgg cgcctgccgg cggcggaccg cgagcgccgt
     6601 gtcgagatcg cctggcacgg cgcgctcgac ccgctcgacg agacgctctt gcaccgcgac
     6661 acgctcgggc agccgcggcc ggtcgcgggc gcgcgcggca gcttcctgcc tggcgcggca
     6721 cggtggcacc cggcgctcgg cgaggcgctc gagggttacc gggtgacgat cgaggttccc
     6781 gagggacagc gcgcgctggt tgccgggcgc ctgctcgaag agcgcgtgag cggcgctcgc
     6841 taccgcgcgc gcttcgaata cccgcatccg gcgcaggata tcgacctgat cgccggcccc
     6901 taccggatcg aggaccgcgc agtgcgcacc gcggcgggca gcgcggtacg cctgcgcacg
     6961 tactttcacc ccgagatcgc gccgctcggc cccggctacc tcgacgcggt gctcggctac
     7021 ctcgatcgct acgaggcctg gattggcgcg tatccgcata cggagttcag cgtggtctcc
     7081 agccccaccc cgacgggatt cggcatgccc agcctcactt acctcggcat cgacgtgctg
     7141 cgcctgccct tcatccgctt tacttcgctc ggccacgagg tgctgcacaa ctggtggggc
     7201 aacggggtgc
//
LOCUS       DBNHKE010000155         3399 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1030991, whole genome shotgun sequence.
ACCESSION   DBNHKE010000155 DBNHKE010000000
VERSION     DBNHKE010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1030991"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..2944)
                     /locus_tag="AC16O9_10395"
     CDS             complement(140..2944)
                     /locus_tag="AC16O9_10395"
                     /inference="COORDINATES: protein motif:HMM:NF017857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HIR6517066.1"
                     /translation="MRRLLLLLLIPGPLALAAEPLALPSATGQTGLVSMPDARFSADG
                     TWRTGYSFLRPYHAIWSSLTAMPWLEGSFRYTRVQYVEGFPGRPGTDYGDYKDKSFDA
                     KIRLLDERAAWPQIAVGAHDVGGGTGLFTAYYAVASKRFGEFDLTLGYGTDRIDGAFG
                     GLRWQPASYPNWSLAAEYDAFDYPNDRFAQQARIADRKKGAALALEYRRDAWGAKAYA
                     AHGEAGVNAWISIPLEAKEFVPKLNEPAPYTRINPRPSEAQWREDPAHEARLRRALAQ
                     QGYDDIHTAYANGRLEATLANTRIASMPRAVGRAARTLLSFAPLEVRELRVTYKQGAL
                     AFATYSFADAKALQRYFNGMTTRERLAQTVAIAYAEPAGAERDADREAALAAFQEPLP
                     EDVVVQDTVPQLFGYRADLAGGTFRIRPGLSTFFNDPSGALKLDLSALATWDRALAPR
                     TFFNSEAKLTLAENVSDVTQPSNSLLPHVRTDVAEYRRAADAKLLKALFNQYTHPAER
                     VYARASLGIYEEMYSGAGGQVLYLAPDGGWAADLAVDALRQRDFEGWGYRDYKTLTAI
                     ASLHYRMAQGLTGTLRAGRFLAKDEGVRVELKRRFASGFEVGAWYTWTNGNDITSPGT
                     PESPYRDKGIFMQMALNAMLTKDTRATANLSLAPWTRDVGQMVVSPGDLARLMERNVF
                     DRHDLDGLRRFGDMEDDYHLPALGARRWPDFLADDARAMGRASGEASWVDGLVLGTGL
                     VLASAALDKPVERYARRHADDKWLERGVKWGDALPVAAIGLSGLFALDESRPRLSDAG
                     VAALEAGALAFAGTEVVKRAVGRARPDQGLGAGEFDAFTNEDRLHSFPSRHAAVMWAA
                     VTPYAKEFGMPWLYGLAALTNAARAGSREHWVSDTVAGSLIGYWLGSIAWEARRDARL
                     RKDGPRLGVGLNNVTVAWDF"
     gene            complement(3010..>3399)
                     /locus_tag="AC16O9_10400"
     CDS             complement(3010..>3399)
                     /locus_tag="AC16O9_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517067.1"
                     /translation="IRGTDFMVALVNPAYVNVISGAVSATNAGGTVVFGQGSIGSIAS
                     ASTLAAPISAAALPAAASSAFSNLAAAQVTAAVTPAAAANAASGAVTGAAAAAPTAGV
                     GAAVAIGVGAAVIGTAVNDTSQTTTHH"
BASE COUNT          490 a         1154 c         1261 g          494 t
ORIGIN      
        1 gcccagaaag ccgagaccat ggaagatcga atcaaggcac cgcggaatct cccggccaaa
       61 aaagccgaga ccatagggct ctgatcgaag gcttctgcgt gttgtcatcg cttgcgtgca
      121 gttaacggca gcatgagcac taaaaatccc acgccacggt cacattattc aatcccacgc
      181 ccaggcgagg gccgtcctta cgcaaccttg catcgcggcg cgcctcccag gcgatcgagc
      241 cgagccagta gccgataagc gaccccgcca cggtgtcgga gacccagtgc tcgcggctgc
      301 cggcgcgcgc ggcgttggtg agggcggcaa ggccatagag ccagggcatg ccgaattcct
      361 tggcgtaggg cgtcacggcc gcccacatca ccgccgcgtg gcgcgacgga aagctgtgca
      421 agcggtcttc gttggtgaag gcgtcgaatt cgcccgcgcc caggccctgg tcggggcgcg
      481 cgcggcccac ggcgcgcttc acgacctcgg tgccggcgaa ggcgagcgcg ccggcttcca
      541 gcgccgccac ccccgcgtcc gagaggcgcg ggcgcgactc gtcgagcgcg aacaggcccg
      601 agagcccgat cgcggcgacc ggcagcgcgt cgccccattt cacgccgcgc tcgagccatt
      661 tgtcgtcggc gtggcggcgc gcgtagcgct cgaccggctt gtcgagggcg gcggaggcga
      721 gcaccagccc ggtgccgagc acaaggccgt ccacccagct cgcctcgccg ctcgcgcgcc
      781 ccatggcgcg cgcgtcgtcg gcgaggaagt cgggccagcg ccgcgcgccg agcgcgggca
      841 ggtggtagtc gtcctccatg tcgccgaagc gccgcaggcc gtccaagtcg tggcggtcga
      901 acacgttcct ctccatgagc cgcgccaggt ccccgggcga gaccaccatc tggccgacgt
      961 cgcgcgtcca gggcgcaagc gacaggttgg ccgtggcgcg cgtgtccttg gtgagcatgg
     1021 cgttcagcgc catctgcatg aagatgccct tgtcgcggta cgggctctcc ggcgtgccgg
     1081 gcgaggtgat gtcgttgccg ttggtccagg tgtaccaggc gcccacctcg aagcccgagg
     1141 cgaagcggcg cttgagctcc acgcgcacgc cctcgtcctt ggcgagaaag cgcccggcgc
     1201 gcagcgtccc ggtgagcccc tgtgccatgc ggtagtgcaa cgaagcgatc gccgtcagcg
     1261 tcttgtagtc gcgataaccc cagccctcga agtcgcgctg gcgcagcgcg tccacggcga
     1321 ggtccgccgc ccagccgccg tccggcgcga ggtacaggac ctgcccgccc gcgcccgagt
     1381 acatctcctc gtagatgccc aggctcgcgc gcgcgtagac gcgctcggcc gggtgggtgt
     1441 actggttgaa gagcgccttc agcagcttcg catccgccgc gcgccggtac tcggcgacgt
     1501 cggtgcgcac gtgcggcagc aggctgttcg agggctgcgt cacgtcgctc acgttctcgg
     1561 cgagcgtgag cttggcctcg ctgttgaaga aggtgcgcgg cgcgagcgcg cggtcccagg
     1621 tggcgagcgc cgagaggtcg agcttgagcg cgccgctcgg gtcgttgaag aaggtcgaga
     1681 ggccgggtcg gatacggaac gtgccgcccg cgaggtccgc gcgatagccg aagagctgcg
     1741 gcaccgtgtc ctgcaccacc acgtcctcgg gcagcggctc ctggaaggcg gcgagcgcgg
     1801 cctcgcggtc ggcgtcgcgc tcggcgcctg cgggctcggc gtaggcgatc gccacggtct
     1861 gcgccaggcg ctcgcgcgtg gtcatgccgt tgaagtagcg ctgcagcgcc ttcgcgtcgg
     1921 cgaagctgta ggtggcgaag gcgagcgcgc cctgcttgta cgtgacgcgg agctcgcgca
     1981 cttcgagcgg cgcaaacgag agcagggtgc gcgcggcgcg gcccacggcg cgcggcatcg
     2041 aggcgatgcg ggtgttggcg agcgtcgcct ccagcctgcc gttggcgtac gcggtgtgga
     2101 tgtcgtcgta gccctgctgc gcgagggcgc ggcgcaagcg cgcctcgtgc gccgggtcct
     2161 cgcgccactg cgcttcgctc gggcgcgggt tgatcctggt gtagggcgcg ggctcgttca
     2221 gcttgggcac gaactccttc gcctcgagcg ggatcgagat ccaggcgttg acgccggctt
     2281 cgccgtgcgc ggcgtaggcc ttcgcgcccc aggcgtcccg gcggtactcg agcgcgagcg
     2341 cggcgccttt cttgcggtcg gcgatgcgcg cttgctgcgc gaagcggtcg ttcgggtagt
     2401 cgaaggcgtc gtactcggcg gcgagcgacc agttggggta gctcgccggc tgccagcgca
     2461 gcccgccgaa ggcgccgtcg atgcggtcgg tgccgtagcc cagcgtgagg tcgaattcac
     2521 cgaagcgctt ggaggcgacg gcgtagtagg cggtaaagag gcccgtgccg ccgccgacgt
     2581 cgtgcgcgcc cacggcgatc tgcggccacg ccgcgcgctc gtcgagcagt cgaattttcg
     2641 cgtcgaagga cttgtccttg tagtcgccgt agtcggtacc ggggcggcca ggaaagccct
     2701 cgacgtactg gacgcgtgta tagcggaacg acccttcgag ccacggcatc gccgtgaggc
     2761 tcgaccagat cgcgtggtag ggcctgagga acgaatagcc cgtgcgccag gtgccgtcgg
     2821 ccgaaaagcg cgcatcgggc atgctgacga ggccggtctg gccggtggcc gaaggcagcg
     2881 cgagcggctc cgccgccagg gcgagcggcc cggggatgag gagcaggaga aggagcctac
     2941 gcatgtccgg aaacgcgatc cagaaaaaga aaaggccccg cggggggcct ttgcctcgtc
     3001 tcttgccgat cagtggtgcg tggtggtttg gctcgtgtcg ttcaccgcgg tgccgatgac
     3061 cgcggcgccg acgccgatgg cgaccgccgc gccgacgccg gccgtggggg cggcagccgc
     3121 ggccccggtg accgcgcccg aggcggcatt ggctgccgcg gcaggcgtca ccgccgcggt
     3181 gacctgcgcc gcggcgaggt tgctgaacgc gctgctcgcc gccgccggca gggccgcggc
     3241 cgaaatcggc gcggccagcg tcgacgcgct cgcgatcgag ccgatcgagc cctggccgaa
     3301 gacgaccgtg ccgccggcgt tggtggcgct caccgcgccg gaaatcacgt tgacgtaggc
     3361 cgggttcacc agggcgacca tgaagtcggt gccgcggat
//
LOCUS       DBNHKE010000156         9313 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_397352, whole genome shotgun sequence.
ACCESSION   DBNHKE010000156 DBNHKE010000000
VERSION     DBNHKE010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_397352"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..684
                     /gene="pth"
                     /locus_tag="AC16O9_10405"
                     /pseudo
     CDS             73..684
                     /gene="pth"
                     /locus_tag="AC16O9_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            825..1919
                     /gene="ychF"
                     /locus_tag="AC16O9_10410"
     CDS             825..1919
                     /gene="ychF"
                     /locus_tag="AC16O9_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6517068.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAAIAAENYPFCTIEPNVGIV
                     EVPDPRLSRIAAIAKPQKVIPAVVEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAH
                     VVRCFEDPNVVHVAGKVDPVSDIETIHTELALADLDSVGKQLAKYAKVAQTGGDKEAQ
                     RLVAVLLKAQAALDAGRPARSVDWSKEELAVLRPFFLLTMKPTMYVANVAEHGFEGNP
                     LLEKVEAYAKAEGAPVVAICAALEAQIADMSDEDKKVFLTDAGMEEPGLNRLARAAYA
                     LLGLQTYFTAGPKEVRAWTVPLGATAPQAAGVIHTDFERGFIRAEVATYDDFVSNGGE
                     QGAKEAGKLRVEGKDYLVRDGDVMHFRFNV"
     gene            1916..2365
                     /locus_tag="AC16O9_10415"
     CDS             1916..2365
                     /locus_tag="AC16O9_10415"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6517069.1"
                     /translation="MSEQLGTAVAEDLPQLVELLGILFTQEHELSPDARKQQRALELI
                     LADPSRARIYVARDEGRAIAMAALHFTTSTAEGGRVAGLEDCVVHPEHRGRGVGEKLL
                     AYVLEQARAEGALRVMLLTDGDNARAQALYGKLGFAPSSMLAMRLKL"
     gene            complement(2367..3854)
                     /gene="gabD"
                     /locus_tag="AC16O9_10420"
     CDS             complement(2367..3854)
                     /gene="gabD"
                     /locus_tag="AC16O9_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - GABA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6517070.1"
                     /translation="MNAPAQAGFAGFSLKDTKLFREQCYIDGQWLSADGKKTFEVRNP
                     ASNETLGSVPDMGAAEAKRAIEAAERAWPAWRAKTAKERAAILRKWYDLMMAAQEDLA
                     QILTAEQGKPLAEAKGEIAYGASFIEWFAEEGKRAYGDVIPTHQADKRVLVIKQPVGV
                     CAMITPWNFPNAMITRKAGPALAAGCTVVLKPAEQTPFSALAMAELAERAGIPKGVLN
                     IITGDAATIGKELCANPKVRKLSFTGSTEVGRILMKQSADTVKKLSLELGGNAPFIVF
                     DDADLDAAVEGAIASKYRNAGQTCVCANRLYVQDGVYDAFAAKLAEKVKGFKVGAGHE
                     AGVVIGPLIDAQGMKKVEQHVADAVGKGAKVLLGGKKHEKGGYFFQPTVLTDVTPEMI
                     VSYEETFGPVAPLIRFKSEDEVIRLANNTEFGLSGYFYSRDVGRIFRVAEAMEVGIVG
                     ANVGIISTEIAPFGGVKQSGLGREGSKYGLEEYLEVKYILIGGIQ"
     gene            3999..5180
                     /locus_tag="AC16O9_10425"
     CDS             3999..5180
                     /locus_tag="AC16O9_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6517071.1"
                     /translation="MGINRFVRGAALGLVAATAAAPALAQDIRIGYAISRTGPFAAGA
                     QITQEPNYIMWAEQVNAAGGLTVGGKKRKVELVGLDDRSEPETMVRTYEKLMGSDKVD
                     LILPPWGTGMNFALMPLAQRHKYPMLSCTATGRKLLSLKNPYFFPLLQQPDVMMEALA
                     TFLKARGTTTAAVIHVDELFGLEQAAALEKEFKAKGIQIVEKKSYPIGVKDLQPVLRD
                     IKAKAPGAFVALSYPPDTFLITGQSKALAFNPNAFYAAVGTAFPIYRDKMGAATVEGV
                     MGLGTWNPKMSPRAKAYFDAHVARWQKEPDRWASAHCWAGLEILQQTVEKVGLDRAKI
                     RAHIAATEFDTVIGKIRFKNGENVATPGVVSQWQNGEFEVVWPPQRATAASQFPKPGW
                     K"
     gene            5214..6083
                     /locus_tag="AC16O9_10430"
     CDS             5214..6083
                     /locus_tag="AC16O9_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517072.1"
                     /translation="MSLWFELAASGLITGGIYAVVAIGLNLQYGLMRILNISHGEFLM
                     LGAYLTWLAHTVLQVNPLVFLPVVALAMFVAGVAIYRLCFYRIVSTAPVVEVIEARSL
                     LVGFGLLYLVQNLAYLTWGADLRGYTFLAEPVEIAGARFSANRLVVFAFAVGTSLLLL
                     AVLRFTLIGKAVRAMMQSPTGAQLVGIDTHVLHPVVFGAGLALAGVAGALLSMVYEIS
                     PAMGEPYTVTALIVIVLGGLGSALGSLLGGFLLGVVETFGMHFTSPSLKMVLSYGVLI
                     VVLLARPKGLFAR"
     gene            6080..6997
                     /locus_tag="AC16O9_10435"
     CDS             6080..6997
                     /locus_tag="AC16O9_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517073.1"
                     /translation="MTLRHGLLAILAGSLALALLPWLLSDFLLALALSCIMYAGLAVS
                     WSMFCGATNYLSLATAAFFGLGAYVTAWGAGALPYGLSVLAGGLVAAAFAALVGAAVL
                     HLRGAYFAVITFGLGELVRHSITYYEKAYEGTVGRIIAEAPESATIYWTVLIVAAAAV
                     ATALLVRESRLGLALRGIGSDEERAQTLGVNARRSKIFAFALSAFFAGALGAAMAVRW
                     TYIDPPTVFNPFIGFQTVLIAMVGGAQTLLGPIVSALVFSLLTEFLRLQFPYLFLVIL
                     GLLLILLVLYLPGGIASLFVRWRWRRGDA"
     gene            6990..7730
                     /locus_tag="AC16O9_10440"
     CDS             6990..7730
                     /locus_tag="AC16O9_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517074.1"
                     /translation="MREPLLSARGITVRFGGLVAVDGVDLDVHAGGLVGIIGPNGAGK
                     TTFFHTLSGVLAPTSGRVVVAGEDLTGRAPHAFAAHGVARTFQTPRVFKEMPALENVR
                     FGLEFAGRTRHHAHDAFDEAETILDFLGLSDAAAVPAGGLTPARQRLLEIGMALGARP
                     RVLLLDEVAAGLTHAEVDATAQLIRRVRDELGLAVIWIEHAVHVLMNYVERVAVLHQG
                     AKIADGDPRAVAKNPAVVEAYLGDEVAA"
     gene            7727..8428
                     /locus_tag="AC16O9_10445"
                     /pseudo
     CDS             7727..8428
                     /locus_tag="AC16O9_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            8552..>9313
                     /locus_tag="AC16O9_10450"
     CDS             8552..>9313
                     /locus_tag="AC16O9_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6517075.1"
                     /translation="MAKGGTREIFEPATGKVLTEVGFADAEDVAEAAQAAAAAQTEWA
                     NTPADQRAAVLRRAAQLLEAHAEALRPWIVRETGSIPPKADFELHMVTGILLEAAAIA
                     TQPPGMMLPSGPKRMSFARRVPHGVDGVISPFNAPLILSSRAVAPALALGNAVVLKPD
                     PSTPVTGGFMLARLFEAAGLPAGLLHVLPGDAAVGEAMIKDPNIAMISVTGSTAAGKR
                     VGQLASEHLKRVTLELGGKNSTIVLDDADLDVAASS"
BASE COUNT         1435 a         3134 c         3276 g         1468 t
ORIGIN      
        1 gccgccgaca aaggcgccga caagggcgcc gagaagtcgg acaagaaaga aaagaagtag
       61 ccgctgtcca agatccgcct cgtcgtcggc ctgggcaacc cgggcaagga gtacgagcgt
      121 acccggcaca atgccgggtt ctggctcgtg gagcggcttg ccctggcccg cggcgtcgcg
      181 ctcaagaagg acaaccacta ccaggcgctg gtgggcaagg acggcggcct gtggctggtg
      241 atgccgcaga cgttcatgaa cgcttccggc cgcgcggtgc aaatgctcgc cgggttcttc
      301 aagatcccgc ccgaggaaat cctggtggtg cacgacgagc tcgacttcgc gccgggcacc
      361 gcgcggctga agcagggcgg cggcatcgcg gggcacaacg gcctgaagga catctcgcag
      421 cggctcggca cgcacgacta ctggcgcctg cgtatcggcg tcggccgccc ggacggcgat
      481 tccggcgccg actacgtgct ggagaagccc cccgccgagg agcgtgaggc gatcgaaggc
      541 gccatcgcgc gcgcgctgga gctcatgccg cagatgatcg agggcgacct gcagggcgcg
      601 atgcagaagc tgcacacgca ggacaagccg gtggtggcga aggccgacgc gcccaagccc
      661 gagccgagga aggccgcgcc gaagaaagcc gaggagaagc agcccgagcc cgggaagcct
      721 gagccgacga aggccgcgaa gaaggcggag gcgaagacgc cccagccgaa ggaggagaaa
      781 cccggcctgc tgaagggcct gctcaagggc ctcaccggca agaaatgagc ctcaagtgcg
      841 gcatcgtcgg cttgccgaac gtcggcaagt cgacgctctt taatgcgctc accaaggcgg
      901 cgatcgccgc cgagaactat ccgttctgca ccatcgagcc caacgtcggc atcgtcgagg
      961 tgcccgaccc gcgcctgtca cggatcgccg cgatcgccaa gccgcagaag gtgattccgg
     1021 cggtggtcga gttcgtggac atcgcgggcc tggtcgccgg ggcctcgaaa ggcgagggcc
     1081 tgggcaacaa gttcctcgcc aacatccgcg agaccgacgc catcgcgcac gtggtgcgct
     1141 gcttcgagga cccgaacgtg gtgcacgtcg cgggcaaggt cgacccggtg tccgacatcg
     1201 agaccattca taccgagctc gcgctcgccg atctggattc ggtcggcaag cagctcgcga
     1261 agtacgccaa ggtggcgcag accggcggcg acaaggaggc gcagcgcctg gtggcggtat
     1321 tgctgaaggc acaggcggcg ctcgatgcgg gccgaccggc gcgctcggtg gactggtcga
     1381 aggaggagct tgcggtgctg cggccgttct tcctgctcac catgaagccc accatgtacg
     1441 tggcgaacgt cgccgagcac ggcttcgagg gcaacccgct cctggagaag gtcgaggcct
     1501 acgccaaggc cgaaggcgcg ccggtggtcg cgatatgtgc ggcgctcgag gcccagatcg
     1561 ccgacatgag cgacgaggac aagaaagtgt ttctcaccga cgccgggatg gaggagccgg
     1621 ggctgaaccg cctcgcgcgc gccgcgtatg cgctgctcgg cctgcagacc tacttcaccg
     1681 ccgggccgaa ggaagtgcgc gcgtggaccg tgccgctcgg ggccaccgcg ccgcaggccg
     1741 cgggcgtgat tcacaccgat ttcgagcgcg gcttcatccg cgccgaggtc gcgacctacg
     1801 acgatttcgt cagcaatggc ggcgagcagg gggcgaagga ggcgggcaag ctgcgcgtcg
     1861 agggcaagga ttacctggtg cgcgacggcg acgtcatgca cttccgcttc aacgtgtgag
     1921 cgaacagctc ggcaccgctg ttgcggagga tctgccgcag ctcgtcgagc tgctcgggat
     1981 tctgttcacg caggagcacg agctctcgcc cgacgcgagg aagcagcagc gcgccctgga
     2041 gctgatcctc gccgatccgt cgcgcgcgcg gatctacgtc gcgcgcgacg agggcagggc
     2101 catcgccatg gcggcgctgc acttcacgac cagcaccgcg gagggcggcc gcgtcgccgg
     2161 gctcgaggac tgcgtcgtgc accccgagca ccgtggcagg ggcgtcggcg agaagctcct
     2221 cgcctatgtc ctcgagcagg cgagggccga gggcgcgctg cgcgtgatgc tgctcaccga
     2281 cggcgacaac gcgcgcgcgc aggcgcttta cggcaagctc ggcttcgcgc cctcttcgat
     2341 gctcgcgatg cggctcaagc tctgagctac tggatgcccc cgatcaggat gtacttcacc
     2401 tcgaggtact cctcgagccc gtacttcgag ccctcgcggc cgagccccga ctgcttgacg
     2461 ccgccgaagg gcgcgatctc ggtggagatg atgccgacgt tcgcgccgac gatgccgacc
     2521 tccatcgcct cggcgacgcg gaagatgcgg ccgacgtcgc ggctgtagaa gtagcccgaa
     2581 agcccgaact cggtgttgtt ggccagccga atcacctcgt cctcgctctt gaagcggatg
     2641 agcggcgcga cggggccgaa ggtctcctcg taggacacga tcatctcggg ggtgacgtcg
     2701 gtcagcacgg tcggctggaa gaagtagccg cccttctcgt gcttcttgcc gcccagcagc
     2761 accttggcgc ccttgccgac cgcgtcggcg acgtgctgct cgaccttctt catgccctgc
     2821 gcgtcgatca gcgggccgat cacgacgccg gcctcgtggc ccgcgccgac cttgaagcct
     2881 ttcaccttct ccgcaagctt ggcggcaaag gcgtcgtaga cgccgtcctg cacgtagagg
     2941 cggttggcgc acacgcaggt ctggcccgcg ttgcggtatt tcgaggcgat cgccccttcc
     3001 accgcggcgt cgaggtctgc atcgtcgaac acgatgaagg gcgcgttgcc gccaagctcg
     3061 agcgagagct tcttcacggt gtcggccgac tgcttcatca ggatgcggcc gacctccgtc
     3121 gagccggtga acgagagctt gcgcaccttg ggattggcgc agagctcctt gccgatcgtc
     3181 gcggcgtccc cggtgatgat gttgagcacg cctttcggga tgccggcgcg ctcggcgagc
     3241 tcggccatgg caagcgcgga gaacggcgtc tgctcggcgg gcttcaggac cacggtgcag
     3301 ccggccgcca gcgccggccc ggccttgcgc gtgatcatcg cgttcgggaa attccagggg
     3361 gtgatcatgg cgcagacccc gaccggctgc ttgatgacca gcacgcgctt gtcggcctgg
     3421 tgcgtcggga tgacgtcgcc gtaggcgcgc ttgccttctt cggcgaacca ctcgatgaac
     3481 gaggcgccgt aggcgatctc gcctttggcc tcggcgagcg gcttgccctg ctcggcggtg
     3541 aggatctgcg ccaggtcctc ctgcgccgcc atcatcaggt cgtaccactt gcgcaggatc
     3601 gcggcgcgct ccttggcggt cttggcgcgc caggccggcc aggcgcgctc ggcggcctcg
     3661 atcgcgcgtt tcgcctcggc ggcgcccatg tcgggcaccg agccgagcgt ctcgttgctc
     3721 gccgggttac gcacctcgaa cgtcttcttg ccgtcggccg agagccactg gccgtcgatg
     3781 tagcactgct cgcggaagag tttggtgtcc ttgagggaaa agccggcaaa gccggcctgt
     3841 gcgggagcgt tcatggggac ctcgctggtt gatgcggaat tctaacgctc ggcaccggat
     3901 gggttgcatg aaccactgtt caatcgtaaa ttgccaagaa accaaaagat tcgagacggt
     3961 cggcagccac ggccgcccca accagagagg agatagcgat gggaatcaat cgtttcgttc
     4021 ggggcgccgc cctgggccta gttgcggcga cggctgccgc gccggcgctc gcgcaggaca
     4081 tccgcatcgg ctacgccatc tcgcgcaccg ggccgttcgc cgccggcgcg cagatcaccc
     4141 aggagccgaa ctacatcatg tgggccgagc aggtgaacgc cgcgggcggg ctcaccgtcg
     4201 ggggcaagaa gcgcaaggtg gagctcgtcg gcttggacga ccgcagcgag cccgagacca
     4261 tggtgcgcac ctacgagaag ctgatgggca gcgacaaggt cgacctgatc ctgccgccct
     4321 ggggcacggg catgaacttc gcgctgatgc cgcttgcgca gcggcacaag tatccgatgc
     4381 tttcgtgcac ggccaccggc aggaagctgc tgtcgctgaa gaacccgtac ttcttcccgc
     4441 tgctgcagca gcccgacgtg atgatggagg cgctcgccac gttcctgaag gcgcgcggca
     4501 cgaccaccgc ggcggtgatt catgtcgacg agctgttcgg cctcgagcag gccgcggcgc
     4561 tggagaagga attcaaggcg aagggcatcc agatcgtcga gaagaagagc tatccgatcg
     4621 gcgtgaagga cctgcagccg gtgctgcgcg acatcaaggc gaaggcgccg ggcgcgttcg
     4681 tcgcgctctc gtacccgccc gacacgttcc tcatcaccgg gcagtccaag gcgctcgcct
     4741 tcaacccgaa cgcgttctac gccgcggtag gcaccgcgtt cccgatctac cgcgacaaga
     4801 tgggcgcggc gacggtggag ggcgtgatgg gccttggcac ctggaacccg aagatgagcc
     4861 cgcgcgccaa ggcctacttc gacgcgcacg tcgcgcgctg gcagaaggag ccggaccgct
     4921 gggcgagcgc gcactgctgg gccgggctcg agatcctgca gcagacggtg gagaaggtgg
     4981 ggctcgaccg cgccaagatc cgcgcgcaca tcgccgcgac cgagttcgac accgtgatcg
     5041 gcaagatccg gttcaagaac ggcgagaacg tggccacccc gggcgtggtg agccagtggc
     5101 agaacggcga gttcgaggtc gtgtggccgc cgcagcgcgc gacggcggcc tcgcagttcc
     5161 cgaagcccgg ctggaagtga tcgcatagcg gcgaaccagg ggctcacggg gccatgagcc
     5221 tctggttcga gctcgccgcc tccggcctca tcaccggcgg catctatgcc gtggtggcga
     5281 tcggcctcaa cctgcagtac gggctgatgc gcatcctcaa catctcgcac ggcgagttcc
     5341 tgatgctggg ggcgtatctc acctggctcg cgcacacggt gctgcaggtg aacccgctcg
     5401 tgtttctgcc ggtggtggcg ctggcgatgt ttgtcgcggg cgtggcgatc taccggctgt
     5461 gcttctaccg catcgtcagc accgcgcccg tggtcgaggt gatcgaggcg cgcagcctcc
     5521 tggtgggctt cgggctgttg tacctggtgc agaacctcgc ctacctgacc tggggcgcag
     5581 acctgcgcgg atacacgttt ctcgccgagc cggtggagat cgcgggcgcg cgcttctccg
     5641 ccaaccgtct ggtggtcttc gcgttcgccg tcggcacgag cctgctgctc ctcgcggtgc
     5701 tgcgctttac gctcatcggc aaggcggtgc gcgcgatgat gcaatcgccc accggcgcgc
     5761 agctcgtcgg catcgacacc cacgtgctgc acccggtggt gttcggcgcc gggctcgccc
     5821 tcgccggggt ggccggggcg ctcctttcca tggtgtacga gatctcgccg gcgatggggg
     5881 agccctacac cgtgaccgcg ctgatcgtga tcgtgctggg cgggctgggg agcgcgctgg
     5941 ggagcctgct cggcggtttt cttctcggcg tggtggagac cttcggcatg catttcacca
     6001 gcccgtcgct caagatggtg ctttcctacg gcgtgctgat cgtggtgctg ctcgcgcgcc
     6061 cgaagggcct gttcgcgcga tgacgctgcg ccacggcctg ctcgccatcc ttgccggcag
     6121 cctcgcgctc gcgctgcttc cctggctgct gtcggacttc ctgctggcgc tcgcgctctc
     6181 ctgcatcatg tacgcggggc tcgccgtgag ctggtcgatg ttctgcggcg cgaccaacta
     6241 cctgtcgctc gccaccgccg ctttcttcgg cctcggggcc tacgtcacgg cctggggcgc
     6301 gggcgcgctg ccttacggcc tgtcggtgct cgccggcgga ctggtggccg cggccttcgc
     6361 ggcgctggtc ggcgccgcgg tgctgcatct gcgaggcgcc tatttcgccg tcatcacctt
     6421 cggcctcggc gagctggtgc gccattcgat cacctactac gagaaagcct acgagggcac
     6481 ggtgggccgc atcatcgccg aggcgcctga atccgccacc atatactgga cggtgctgat
     6541 cgtggccgcg gcggcggtgg cgacggcgct cctggtgcgc gaaagccgcc tcgggctcgc
     6601 gttgcgcggc atcggctccg atgaggagcg cgcgcagacg ctcggcgtga acgcgcgccg
     6661 cagcaagatc ttcgccttcg cgctgtcggc gttcttcgcc ggcgcgctgg gcgcggcgat
     6721 ggcggtgcgc tggacctaca tcgacccgcc cacggtgttc aacccgttca tcggcttcca
     6781 gaccgtgctg atcgcgatgg tcggcggcgc gcagaccctg ctcggaccga tcgtctcggc
     6841 gctggtattc agcctgctca ccgagttcct gcgccttcag tttccgtacc tgttcctcgt
     6901 catcctgggg ctgctcctca tcctgctggt gctctacctg cccggcggca tcgcctcgct
     6961 cttcgtgcgc tggcgctgga ggcgcggcga tgcgtgagcc gctgctttcg gcgcgcggca
     7021 tcacggtgcg ttttggcggc ctggtggcgg tcgacggcgt ggatctcgac gtgcacgcgg
     7081 gcgggctggt gggcatcatc ggcccaaacg gggcaggcaa gacgaccttc ttccacacgc
     7141 tctcgggggt gctggcgccc acttcggggc gcgtcgtcgt cgcgggcgag gacctcaccg
     7201 gccgcgcgcc gcacgcgttc gccgcgcacg gcgtggcgcg caccttccag actccgcgcg
     7261 tgttcaagga gatgccggca ctcgagaacg tgcgcttcgg gctggagttt gccggccgca
     7321 cgcggcacca tgcgcacgat gcattcgacg aagccgaaac gatcctcgat ttcctcggat
     7381 tgtcagacgc cgcggcggtg ccggcgggcg ggctcacgcc tgcgcgccag cgcctgctcg
     7441 agatcggcat ggcgctcggc gcacgcccgc gcgtgctgct gctcgacgag gtggccgcgg
     7501 ggctcacgca tgccgaagtc gacgccaccg cccagctcat ccgccgggtg cgcgacgagc
     7561 tggggctcgc cgtgatctgg atcgagcatg cggtgcacgt cctcatgaac tacgttgagc
     7621 gcgtcgcggt actgcaccag ggcgcgaaga tcgccgacgg cgacccgcgc gccgtggcga
     7681 agaacccggc cgtcgtggag gcctacctgg gcgacgaggt cgcggcgtga gcggcggcct
     7741 gtccatcaag gggctctccg cgggctacgg cccgatccgc gtactgcaga acgtggacct
     7801 ggaggcgccc tcgggggaga tcaccgtcgt ggtgggtccc aacggggcgg gcaagacgac
     7861 gctgctgaag gcggtctcgg gcctcattcc gcgcgaaggc gaggtcgtct tcgaaggcga
     7921 agccctgccg gcgcagcccg cgatcatcgt gcgccgcggc ctcgcgcagg tggccgaagg
     7981 ccggcagctc ttcccgcaga tgacggtgcg cgagaacctt gagctcggcg gctacctcct
     8041 gccgcgcggc gagcgcgccg cgcgcctcga gcgcgtgttc gacttcttcc ccaagctcgc
     8101 gcagcgccgc gagcagctcg ccggcaccct gtcgggcggc gagcagcaga tgctcgccgt
     8161 ggggcgcgcg ctgatgggca agccgaagct cctcatgctc gacgaaccct cgctcggcct
     8221 cgcgccgaag atgatcgacg agctgctcgg catcgtgcgg cgcatccgcg acgacggggt
     8281 gaccatcctg ctcgtcgagc agaacatcgc caaggcgctg gcgatcgccg agggcgccta
     8341 cgtgatcgag cgcggcgccg tggtgatgaa gggccaggcg ggcaagctcc tcaaatcgga
     8401 gcgcctgcgc gcggcctacc tcggcgccga caccaaaccc ggaacattga cctggaaggg
     8461 aggcctcttc atggccgata agcagaccac cgcagtgacc atcgccgccg agaacgcctg
     8521 gaagggcaaa gtcttcaccg gccgctggca gatggcgaag ggcggcacgc gcgagatctt
     8581 cgagcccgcc acgggcaaag tgctgaccga agtcggcttc gccgacgccg aggacgtggc
     8641 cgaggcggcg caggcggccg ctgcggcaca gaccgagtgg gcgaatacgc ccgccgacca
     8701 gcgcgcggcg gtgctgcggc gcgcggcgca gctgctcgaa gcgcatgccg aagcgctcag
     8761 gccctggatc gtgcgcgaga cggggtcgat cccgccgaag gcggatttcg agctgcacat
     8821 ggtgaccggg attctgctgg aagccgccgc gatcgccact cagccgccgg gcatgatgct
     8881 gccctcgggc cccaagcgca tgagcttcgc gcgacgcgtg ccgcacggcg tggacggcgt
     8941 gatctcgccg ttcaacgcgc cgctcatcct ctccagccgc gcggtggcgc cggcgctcgc
     9001 gctcggcaac gcggtggtgc tgaagcccga cccgagcacg ccggtgacgg gcggcttcat
     9061 gctcgcgcgt ctcttcgagg cggccggact gcccgccggg ctgctgcacg tgctgccggg
     9121 cgacgccgcg gtgggcgagg cgatgatcaa ggatccgaac atcgccatga tctcggtcac
     9181 cggctcgacc gccgccggca agcgcgtagg gcagctcgcg agcgagcacc tgaagcgcgt
     9241 cacgctcgag ctcggcggca agaactccac catcgtgctc gacgacgccg atctggacgt
     9301 tgcggcttcg agc
//
LOCUS       DBNHKE010000157        12916 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_556075, whole genome shotgun sequence.
ACCESSION   DBNHKE010000157 DBNHKE010000000
VERSION     DBNHKE010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_556075"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            657..1301
                     /locus_tag="AC16O9_10455"
     CDS             657..1301
                     /locus_tag="AC16O9_10455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR6517076.1"
                     /translation="MKQPLDDLAIVTAKTLEHYNERAAEFWSGTRNHNVKQNVEALLR
                     HIRGTPPLCILDFGCGPGRDLVAFRALGHEPIGLEGSPPLAAMAREHSGCEVWEQNFL
                     ALKLPACRFDGIFANASLFHVPRQELPRVLGELHAALTPDGVLFTSNPRGNNEEGWNR
                     GRYGAYHDLESWRAFMKTAPLAELEYYYRPPGLPLERQPWLASVWRKCSEAGAI"
     gene            1623..3302
                     /locus_tag="AC16O9_10460"
     CDS             1623..3302
                     /locus_tag="AC16O9_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6517077.1"
                     /translation="MLTTEDVRAVPLFSTLAEPELERLARTSADLHLAPGEFAAHEGA
                     EERALYVVLSGRMEVVKLFDGVERRLGWRVPGGFFGELPIAIGTPLYGSYRASEPSRV
                     MRVGIREYYAIAAASPEIAEKVAALARERIGGMQGIAAETPKPRVTMVGHRWDKACSD
                     LREFLARNQISHEWITPDAPDLGARWPGPPPADGDCPVLLLADGTRLSRPAMRELAQH
                     LGLQTQARLAEYDTVVIGGGPSGLAAAVYGASEGLRTLVIEREAPGGQAGTSSRIENY
                     LGFPSGVSGDELASRALKQGMRLGAEILVTRAVDRIDPQTREVQLDGGDVVRARTIIL
                     ATGVSWRRLAIEGFDRLIGKGVYYGAARSEAAGTHGLDIHLIGAGNSAGQAAMFFANH
                     ARTVTLVVRGDSLDKSMSHYLVEQLAGKSNVKVRLRSEVVCAYGDTHLTAIDIRDNAS
                     GEVRREDCGGVFVFIGADAQTAWLPQSIARDARGYVLTGDDVVKAGRWPLERDPYLLE
                     TSVPGIFACGDVRLSPVKRVAAAVGEGSMAIAFVHKFLQPALGSPLRARGS"
     gene            3302..4432
                     /locus_tag="AC16O9_10465"
     CDS             3302..4432
                     /locus_tag="AC16O9_10465"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6517078.1"
                     /translation="MKSRVLVLGAGFGGMELSTLLAEALGEQAGVTVIEKRDAFVFGY
                     SKIDVMFGKATLDSVRLPYRNFVKAGVRLVHETITAIDPVARRVTTSGGTYEGEFLVV
                     ALGADYDLAATPGLAEDGNEFYSVAGASRVREVLPAFSKGPAIVGVSGFPFKCPPAPS
                     ECVLTLHHYLATRGVRGACDLTLVLPLSSPMPASADVSKALVGAFAETGITLLLGRSV
                     TALEPGRKSAVLDDGTELPYALYLGVPKHHVPQPVLESGLAENGWVPIDVRTLETRFP
                     EVYAIGDGAATGLPKAGSFAESAARAVASSILAKLGIGEAAPNAGTGSCLVEFGDGRL
                     SWADLDFLTGPKLKSIFHPPSVAGRAHKVQFGASRRARWFGL"
     gene            4674..5378
                     /locus_tag="AC16O9_10470"
     CDS             4674..5378
                     /locus_tag="AC16O9_10470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517079.1"
                     /translation="MKVCIVGASGKLGKYMVQHALDRGYEVVGVCRERSAGKLDGFKE
                     RMTIIPGATDDRDAIRKAVAECDGVLTVLVPWGVQHYSTRTAQAVLDYAGPGARLIFS
                     CGWHITRDGKDVYSLSLKMFVALFGRLARLARFADIDDQVEACRRVFASDTLWTVVRG
                     SDLEEGESQGLPVWSRHAGDPILASNITRRVDFALFMVAALENDALVHEAPAIVGRQT
                     PSARAHAAAVGPGDDR"
     gene            5511..5801
                     /locus_tag="AC16O9_10475"
     CDS             5511..5801
                     /locus_tag="AC16O9_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6517080.1"
                     /translation="MAIYLLLDSTIHDPEKYAAYMRAVPPLVAKHGGQYLARGGKFEV
                     LAGNWKPDRIVLFKWPNREALRAFMSDPDYQEWKKLRESVTTANNRLLIEGV"
     gene            6268..7347
                     /locus_tag="AC16O9_10480"
     CDS             6268..7347
                     /locus_tag="AC16O9_10480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6517081.1"
                     /translation="MDKDKSTRFMLKLVGDVGTALSAGLLVVGDQSGLFRAMAGAGPL
                     SSEALAGRTGISPRYVEEWLAVMSGANYVEHDPGTNTFSLPDEHALFLTDPSSEYYFG
                     GLFQGLPGLLAMTPKLVDAFKTGKGISFADFGSDLPCSLEAMNRQVYENRLVRSWLPA
                     MPEVVRRLEAGGRAIDVGCGTGVIPITIAKAFPSATVAGLDFDARSIGIARGYARESG
                     LGDRVSFLAEPIEALPSEPGWDLISSFDVIHDLPDPLGAMKRIRSALNAGGTYLMVEP
                     KVSDQLEKNVQNPFARMFQAMSCLHCVPQSLAQGGPGLGACWGEGRARAMASDAGFAS
                     FERLDIRSPAMAFYALRADRGNSLG"
     gene            7561..7779
                     /locus_tag="AC16O9_10485"
     CDS             7561..7779
                     /locus_tag="AC16O9_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR6517082.1"
                     /translation="MSDADVAEILKLPPEERLRLAEVIWASLAAEPSTVPLGDAHRQV
                     LDERLAQHDKSPDDVVSRDQVLADARRR"
     gene            7980..8087
                     /locus_tag="AC16O9_10490"
                     /pseudo
     CDS             7980..8087
                     /locus_tag="AC16O9_10490"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8198..8794
                     /locus_tag="AC16O9_10495"
     CDS             8198..8794
                     /locus_tag="AC16O9_10495"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR6517083.1"
                     /translation="MIFEHLGKSPVVDPSAYIAPNAVLCGDVTVGPGARIMFGAQVIA
                     EGGSIRIGRECIVMENAVLRSSTAHHLSIADNCLVGPNAHVVGCTIEEEAFLATGCAV
                     FHGSYIGRGAEVRVNGVVHLKTRLAPGATVPIGWVAVGDPAQMFPASEHEAIWKIQEP
                     LNFPLTVYGFERSEASMVKITKRLSQALGSHLNDKDAV"
     gene            8968..9696
                     /locus_tag="AC16O9_10500"
     CDS             8968..9696
                     /locus_tag="AC16O9_10500"
                     /EC_number="4.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6517084.1"
                     /translation="MRAAADSFAFHPELRDKISDPLTSPSRTLTTAKLAAVSKERGLP
                     LDWWYSDSEREAERAEILAGRRDTDLWVFGYGSLMWDPAFRFAEVRRARISGYARRFI
                     LKDIYGGRGTVDAPGLMVALDKGPACDGLAFRISRENIEEETEALWRRERIGPAYTPV
                     FVEAVAADSRMMALTFVADHEADLIDASLTRAQQIEYCASGTGFMGSSLDYLKNINRQ
                     FSALGIQDDEVSELLREAEAYINS"
     gene            9963..10274
                     /locus_tag="AC16O9_10505"
     CDS             9963..10274
                     /locus_tag="AC16O9_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517085.1"
                     /translation="MMVARWSIDAKFGYKQNAIDLMQRWLREIAPQVGFSAEKTRLLT
                     GSIGALEATIQTEHLVKDLNELNQAWDKLATVAAHRQWSKDLEPHVVSGTSRWEIYRV
                     L"
     gene            10332..10817
                     /locus_tag="AC16O9_10510"
     CDS             10332..10817
                     /locus_tag="AC16O9_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517086.1"
                     /translation="MAKALAVTPYALGMTGMTVVEQSAGKVKALNLNGIAPAEDDVRS
                     GRYFLARDFLFVIKGEPTGPVNRFLDLVMSSCMAYPYFVMWFGGRQSYRRHARRFAPQ
                     CPILFIHGRRKPSRFHSEARADELLTRKGNQVLEFDTGHWVMSEQPERFNQVVSSWVS
                     Q"
     gene            10948..11373
                     /locus_tag="AC16O9_10515"
     CDS             10948..11373
                     /locus_tag="AC16O9_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6517087.1"
                     /translation="MNIQEFMQGYKVAWEARDPAMFAALFHADGKYHNTPFQVQTGKP
                     ELAEYWRRVQLQEEVQVSFDVLASQGDMGIAHWHVTYRVGSEELFQVWARSTGTSLPA
                     RKPGDPLPRMVLDGVLQARFAQGLCTEARIWWHSLPQPA"
     gene            11370..11621
                     /locus_tag="AC16O9_10520"
     CDS             11370..11621
                     /locus_tag="AC16O9_10520"
                     /inference="COORDINATES: protein motif:HMM:NF025607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HIR6517088.1"
                     /translation="MSRAAPPDTSSEPTHSFPMRRVDQLFLGVAGAVFLGLGSFNLLF
                     PAAGMAGFEMQVTNVSALNEARANYGGWRAPAPGAKLAR"
     gene            11618..12124
                     /locus_tag="AC16O9_10525"
     CDS             11618..12124
                     /locus_tag="AC16O9_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517089.1"
                     /translation="MTRIALLALALAAAPAAAEEFAFAVTAPAFKVTIPNLPALEMAP
                     HPMQATHPHLRFLGTKGAYTVSVITARAAAGMTPLECAGAILRSLSERPNVPPAAELH
                     RARLDDHTYAVMYAAQLPGFLQLNAHLLSAAAGTHCIEVHAAKIATSLEDIDPWFKSL
                     GEARIAPE"
     gene            complement(12125..12490)
                     /locus_tag="AC16O9_10530"
     CDS             complement(12125..12490)
                     /locus_tag="AC16O9_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6517090.1"
                     /translation="MAIRYLAAAAAFATMSLGAGSALAQDPVQTDGDKYKVVLENDRV
                     RVLEYRDRPGEKTHQHRHPAFVLYALSPFKRSITLPNGKVLQREFKGGEVMWSEEQTH
                     IGANVGDTETRVLMIELKK"
     gene            12590..>12916
                     /locus_tag="AC16O9_10535"
     CDS             12590..>12916
                     /locus_tag="AC16O9_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6517091.1"
                     /translation="MHPRSPPSFTVGEWRADPGLNCLARPGHAPRRLEPKVMEVLGYL
                     AAHAGEVVPRQELLQALWPGVVVGDESLTQTVIKLRKALGDASREPRYIETIAKRGYR
                     LIAAVGH"
BASE COUNT         2269 a         4054 c         4272 g         2321 t
ORIGIN      
        1 cctccgcaac cattccgaaa atgcagggct caatgtcgat cctgatgttg tggcaagcat
       61 cgctgggcgt tcagatgcct caggctattg atacgggtat tgcagtgatt acacaggaga
      121 acgtggatag atatctggct gatgcaatgt aaaggcccga tatcccatgc caggtcctca
      181 acacaatttg gcaattgagg gtgcaaggcg cgtgcctaac aagcgcttcg aggccggcgc
      241 cttactcctc cgctccgcta cgctgcgttc caggctaagg cgcggctcaa gcacacgtta
      301 gaacgcatcg aatggatttg cgcgcgaaac gccgaatgag gaggaattag gatgacccta
      361 tgcccgatcg ctttagcagc cggttgcaag aagtgtccag cagtcgcaat ctgccctctg
      421 aaaagcgtca ttggcgattt ccagaaaccg ggagagacgc aaacgaagca ggacgccgag
      481 aagtctcgcg gtgccggaac gcgggataag taaccgcaga cgacaagggc gcaaagcgtt
      541 tccctgtcct gcgttctaac ttcacgtcga acggacgtgc cgtgccgcac gcccgcctcg
      601 tagcggcgcg cggttcacga cgacgttaga tctcatcaac ggctagaagg atcgcgatga
      661 aacagccgct cgacgatcta gcgatcgtta cagcgaagac gctcgagcac tacaacgagc
      721 gcgccgccga attctggagc ggcacgcgca accacaatgt gaagcaaaac gtagaggcgc
      781 tgctgcggca tattcgcggt acgccaccgt tgtgcatact cgatttcggt tgcggacctg
      841 ggcgcgactt ggtcgcgttt cgcgctctcg gccacgagcc gataggcctt gagggttctc
      901 cgccgctcgc cgccatggca cgagagcaca gcggctgcga ggtctgggag cagaatttcc
      961 tcgcgctgaa gcttcccgcc tgccgcttcg acggcatctt cgccaacgcc tcgctgttcc
     1021 acgtgccgcg ccaagagctg ccgcgcgtat tgggcgaact gcacgcggca cttacgcccg
     1081 acggcgtgct cttcacttcg aatccccgcg ggaataacga ggagggctgg aatcgcgggc
     1141 ggtacggggc gtaccacgac ctcgaatcgt ggcgcgcatt catgaaaacg gcgccgttgg
     1201 cggagctgga gtattactat cgtccaccgg ggttaccgct cgagcgtcag ccttggctcg
     1261 caagcgtctg gcgaaagtgc agtgaagccg gtgcgatcta acagcgcgtt gcagcggacc
     1321 gtggggagat cttgcttcgc ttgcggccgg cgaacggccg ctcaacgcga acgcggggcg
     1381 tcgagagcag ggatgcgcgc gtccgtaccg tgaatctggg acgagcgggt ccaaacagca
     1441 accacgggac ggctggcggg agtcgggcgc aggcaacgtg ctcaggcgca gatcggcaac
     1501 tcgccagaac ggggacgctg ggccccaagg cgatcggttt gcacatgctt tgtcttgctg
     1561 gatgacgatg agcgtgcaat cgtctatttt ccctcccatt tcctcctctt gctgaccaaa
     1621 ccatgctgac cactgaggat gtccgggcag tcccgctgtt ctccacgctc gccgagccgg
     1681 aactcgagcg gctcgcacga acgtccgccg acttgcacct cgcgcccggg gaattcgccg
     1741 cgcatgaggg cgcggaggag cgggcgctgt acgtcgtcct ctccggcagg atggaggtcg
     1801 tcaagctgtt cgacggagtc gagcgcaggc tcggctggcg cgttccaggc gggtttttcg
     1861 gcgaactgcc gattgcgatc gggacgccgc tgtatggaag ctatcgtgcg tccgaaccct
     1921 ctcgcgtcat gcgcgtgggc atccgcgagt actacgcgat cgccgcggca tcgcccgaga
     1981 tcgcggagaa ggtggcggcg ctggcgcgcg agcgcatcgg cggaatgcag ggcatcgcgg
     2041 ccgagacacc caagccgcgt gtgaccatgg tgggacaccg ctgggacaag gcctgcagcg
     2101 atctgcgcga gttcctcgcc cgcaaccaga tctctcacga atggatcacg cccgatgcgc
     2161 ccgacctggg ggcgcgctgg cccggacctc ccccggcgga cggagattgc ccggtgctgc
     2221 tgcttgccga cggaacgcgc ctcagtcggc ccgcgatgcg tgaactcgcg cagcacctcg
     2281 gcctgcaaac gcaggcgcgg ctcgccgaat acgacaccgt ggtcatcggc ggcggcccat
     2341 cgggcctggc ggcggcggtg tacggcgcgt ccgagggatt gcgcacgctc gtgatcgagc
     2401 gcgaggcacc cggtggccag gccggtacct cttcgcgcat cgagaactac ctcggcttcc
     2461 cgagcggggt ctcgggtgac gagctcgcga gccgagcgct caagcaggga atgaggctcg
     2521 gtgcggagat cctggtcacg cgcgcggtcg atcgcatcga tccgcaaacg cgtgaggtcc
     2581 aactcgatgg cggggatgtc gtccgggcgc gcaccatcat ccttgcgacg ggagtctcgt
     2641 ggcgccgcct cgcgatcgag ggattcgatc gactgatcgg caagggcgtc tactacggcg
     2701 ccgcgcgcag cgaagccgcg gggacgcacg gactcgatat ccacctcatc ggggccggaa
     2761 actcggccgg ccaggccgcc atgttctttg ccaatcacgc gcgaaccgtg acgctcgtcg
     2821 tccggggcga ttcgctggac aagagcatgt cgcactacct cgtcgagcaa ctcgccggga
     2881 agtccaacgt gaaggtgcgg ctgcgctcgg aagtcgtgtg cgcgtacggc gacacgcacc
     2941 tgacggcgat cgacatacgc gacaacgcaa gcggcgaggt acgccgcgag gactgcggtg
     3001 gcgtgtttgt cttcatcggc gcggacgccc agaccgcatg gcttccgcag agcattgcgc
     3061 gcgatgcgcg cggctacgtc ctgactggcg acgacgtggt gaaggccggg cgctggccgc
     3121 tcgagcgcga tccctacctg ctcgaaacga gcgtcccggg gatcttcgcg tgtggggacg
     3181 tgcggctgag cccggtcaag cgcgtcgccg ccgcggtcgg cgaaggcagc atggcgatcg
     3241 cgttcgtcca caagtttctg cagccggcgc ttggcagccc gttgcgggcg agaggatcgt
     3301 gatgaagtcg cgggttctcg tgctgggtgc cggtttcggc ggcatggagc tgtccaccct
     3361 gttggcggag gcgctcggag aacaggccgg cgtgaccgtg atcgaaaagc gggacgcctt
     3421 cgtcttcggc tattccaaga tcgacgtgat gttcggcaag gcgacgctgg attcggtgcg
     3481 cctgccctac cgcaacttcg tcaaggccgg cgtgcgcctg gtgcacgaga ccatcaccgc
     3541 gatcgatccc gtggcgcggc gcgtgaccac cagcggcggc acctacgagg gcgaattcct
     3601 ggtggtcgcg ctgggcgccg actacgacct cgccgcgacg ccgggccttg ccgaggacgg
     3661 caacgagttc tactcggtgg cgggtgcaag ccgggtgcgc gaggtcctgc ccgcgttttc
     3721 caagggcccg gcgatcgtgg gcgtgtccgg ctttccgttc aagtgcccgc cggcgccgag
     3781 cgagtgcgtg ctgacgctgc accactacct tgcgacgcgc ggcgtgcgcg gcgcgtgcga
     3841 cctcacgctc gtactgccgc tctccagccc gatgccggcc tcggcggacg tgtccaaagc
     3901 gctcgtcggc gcgtttgccg aaacgggcat cacgctgctg cttggccgct cggtcacggc
     3961 actggagccg gggcgcaaga gcgccgtgct cgacgacgga accgagttgc cctacgcgct
     4021 gtacctcggg gtgccgaagc accacgtgcc gcagccggtg ctggaaagcg ggctggccga
     4081 gaacggctgg gtgccgatcg acgtgcgcac gctggagacg cgcttccccg aggtgtatgc
     4141 catcggcgac ggagccgcga ccgggctgcc gaaagccggg tcgtttgccg agagcgcagc
     4201 ccgcgccgtg gcgagctcga tcctcgccaa gctcggcatc ggcgaggcgg cacccaatgc
     4261 gggaacgggc agttgcctgg tcgagttcgg cgacggtcgc ctcagctggg cggacctgga
     4321 ctttctgacc gggccgaagc tgaagagcat ctttcacccg ccctcggtcg ccggacgggc
     4381 ccacaaggtg cagttcggtg ccagccgcag ggctcgctgg ttcggcctct gacacatgcc
     4441 cttcagttcg tcaccgaacg cgctctggcc acccctgccg ggggttgcgc aggctgaacg
     4501 aggtcaacgt gatctggcat gacgttgatc atgtttgctc cacgagcggg atcggcacgc
     4561 cgacgcccga cccgtcgatg caccggacct gctgcagcgg gcttcgcccg ctttcgcggg
     4621 cgggtgatct ctagcgtcat gtggcagacc gttggacgtg agggcagcgc tcgatgaaag
     4681 tgtgcatcgt cggggcatcc ggcaagctcg gaaagtacat ggtgcaacac gcgcttgacc
     4741 ggggctacga ggtcgttggc gtttgccgtg agcgcagcgc aggcaagctt gacgggttca
     4801 aagagcgcat gacgatcatt cccggcgcga cggatgatcg cgacgcgatc aggaaagccg
     4861 tcgcggagtg tgacggggtg ctcacggtgt tggttccttg gggggtccag cattactcga
     4921 ctcgaacggc gcaggcggta ctcgactacg caggtccggg cgcgcggctc atcttttcct
     4981 gcggctggca tatcacgcgg gacggcaagg acgtgtactc gctgtccctc aagatgttcg
     5041 tggcgctctt cggccggctt gcgcggcttg cccgcttcgc ggatatcgac gaccaagtgg
     5101 aagcgtgccg gcgggtgttt gccagcgaca cgttgtggac ggtcgtgcgt ggaagcgacc
     5161 tcgaagaggg tgagagccag ggcctgccgg tgtggagccg ccacgcgggc gaccccatcc
     5221 tggcgagcaa catcacgcgg cgcgtggact tcgccctgtt catggtagcg gcccttgaga
     5281 acgacgcgct tgtccacgag gctccggcaa tcgtcgggcg ccaaacccca tcggcccgcg
     5341 cgcatgccgc tgctgtcggg ccgggagacg atcgttgatg aagcgcggcc agcagtctcg
     5401 ctcagtagtg ccacataatc cgcgtcatgc ggcatagatc gcgggcaggc aacgcctgcg
     5461 ttcgactcaa aacggacact ctggaatagc ctctctcaaa ggagcaacgc gtggcgatct
     5521 atctccttct tgacagcact attcacgatc ctgagaagta cgcagcatac atgcgcgcgg
     5581 tgcccccgct ggtggcgaag catggcgggc aatatcttgc ccgaggcggc aagttcgagg
     5641 ttcttgcggg aaactggaag ccggaccgca ttgtcctttt caagtggccg aatcgcgagg
     5701 cgcttcgcgc cttcatgtcg gatccggact atcaggaatg gaaaaagctg cgtgaatcag
     5761 tgacgacggc aaacaaccgg ttgctcatcg agggcgtgta gcgcgccgga cttgcgaatg
     5821 cctgctcttg gtcgattacg gcccatcgca tttgacatca atcatgctgc atgacaatcg
     5881 cctcgagccg acaacctccg cagggcttcg ccctcctccg catgcggctc cagcgccacg
     5941 ttaggcatct gcatgacctc acggacacaa ctacagctgg cgtggaccac ctttgccctc
     6001 ttcgtcgccg cggctctcct cgctggcgag ttgggcacag gttccgggtg gggcatcggt
     6061 gcggttgtcg tgcttggtgc cttgagtcta gttccagggc tttgggcgct cgcgcgaagc
     6121 aacttcacac gcgcaagtgt cgtgctcgtg gcccttggcg tggcggtcag ccaatggcgc
     6181 acgctccaga tgttgtacct tgtcacgtca tggggagcgc atggggctca aggcgtggcg
     6241 tttgaagcgc caacttagga gatgcgcatg gacaaggaca aatccacccg cttcatgctg
     6301 aagctcgttg gcgatgttgg cacggcgctg tccgccggac tgctcgtcgt cggcgatcaa
     6361 agtggcctgt tcagagccat ggccggggcg gggccgttgt cctccgaggc gcttgccggg
     6421 cgcaccggca tttccccccg ctatgtcgag gaatggctcg ccgtgatgag cggcgcgaac
     6481 tacgtcgagc acgaccccgg caccaacacg ttttccctcc cggacgaaca tgctcttttc
     6541 ctcaccgacc cgtcgtcgga atattatttc ggcgggttgt tccagggtct tccgggactg
     6601 ctcgcgatga cgccgaagct ggtagatgcg ttcaagacgg gcaaggggat ctcgtttgcc
     6661 gatttcggct cggatttgcc ctgttcgctg gaggcgatga accggcaagt ctacgagaac
     6721 cgcttggtgc gcagttggct gcccgcaatg ccggaggtgg tgaggcggct ggaagcagga
     6781 ggacgcgcaa tcgacgtggg ctgcggaaca ggcgtcattc ccataacgat cgccaaggcc
     6841 tttccgtcgg cgacggtcgc cgggctcgac ttcgacgcac gttccatcgg gatcgcacgc
     6901 ggctacgcgc gtgaatccgg attgggcgac cgcgtctcct ttcttgccga gcccatcgag
     6961 gcgctgccga gcgaacccgg atgggatctg attagctcgt tcgacgtcat ccacgacctg
     7021 cccgacccgt tgggtgcaat gaagcgcatc cgttccgctc tcaatgcggg cggcacgtac
     7081 ctgatggtcg agccaaaggt atccgaccag ctggaaaaga acgtccagaa cccgtttgcg
     7141 cgaatgtttc aggccatgag ctgcctgcac tgcgtgccgc agtcgctcgc gcagggcggt
     7201 ccgggtctgg gcgcgtgctg gggcgagggg cgcgcccgcg ccatggcgag cgacgctggg
     7261 ttcgcgagct tcgagcgcct cgacattcgc agccctgcca tggccttcta tgccctgcgc
     7321 gcggaccgtg gcaacagcct cggatgaccg gagccaaccc gtcgagagct ctcgccgcag
     7381 cgctgatcac gagcgtgcgc caccgaacaa cggcttgcag ggagacgcgc cgtgcgcggc
     7441 gatgcctaac atggggctgc agccgacgcg ctacagcgtg ccgccactcg gtcgcgacgc
     7501 ctccgcgcgc ggctgagctc tacgttagcg cgcagataac tggagtggta ggctactcat
     7561 atgagtgatg ccgacgttgc cgagatcctg aagctcccgc ccgaggagcg cctacgcttg
     7621 gccgaggtca tctgggccag tctggccgcg gagccttcca ccgtgcccct gggtgacgcc
     7681 caccgccaag tgcttgacga acggctggcc caacacgaca aaagccccga cgacgtagtt
     7741 tccagagacc aagtgctcgc cgacgcccgg cgacgctgat gtctctgccg gtcgtctacc
     7801 gtcggcaggt cgggcgggat cttgcagctg ggtttgggta ctacgaaggg caagcgaaag
     7861 ggctgggcga gcgttttcta actgctgtcg gttccgcgtt tgatgccatc gagcgctatc
     7921 cgcagatgtt tgcgtaggtc cacggcgagg tacgccgcgc cctcctatcg cagtttccgt
     7981 atggtgtctt ctaccgcgtc gaatccaagc gggtggtcgt tctggccgtc ttgcacacgg
     8041 cgcgcgatcc aagcctctgg ccgcggcctc gaaggcctgc gcgctgacag cgaggcgcaa
     8101 agcgacgctt ttggctcggc gctggtcgcg cctccgccca gcgcgcctgg ccgcgaacgt
     8161 caggcggcgt aaagacgcgt gcgtccataa tagccgcatg atcttcgaac atcttggcaa
     8221 gtcgcccgtc gtagatcctt cggcgtatat cgccccgaac gctgttctgt gcggggacgt
     8281 tactgttggc ccgggcgccc ggatcatgtt cggcgcccaa gttatcgcag agggtgggtc
     8341 tatacgcatc ggccgcgaat gcatcgttat ggaaaacgca gtcctgcgta gttcaacggc
     8401 tcaccacttg agcatcgccg acaattgtct cgttggacca aacgcgcatg tggtcggatg
     8461 cacgatcgaa gaagaagcct ttctggccac cggttgcgcc gtgtttcacg gctcgtacat
     8521 cggcagaggc gccgaagtcc gcgtcaacgg cgttgttcat ctgaagaccc ggctcgcccc
     8581 cggcgcaaca gttccgatcg gatgggttgc cgttggagat cctgcgcaaa tgttccccgc
     8641 cagcgagcac gaggcgatct ggaagattca agagccgctg aactttccgc ttaccgtcta
     8701 cgggtttgag cgaagcgaag cgagcatggt gaagataacc aaacgtcttt cgcaggcgct
     8761 ggggagccat ctcaatgaca aagatgccgt ctaaccccgc agcccacgcg gacgcgcgcg
     8821 gaactcccat gctttgcagg gctcaccggg cgcgcggcgg tgggcgcgga cgttagtgct
     8881 cgactcgacg gcggcgcgat gcggcaagat gcatcatcgc gttatggcca ctctgactcc
     8941 caattcgacc aaacttggag gcttgctatg agggctgcgg cggactcctt cgcctttcac
     9001 ccggaactgc gcgataagat ttccgatccc ttgacgtcgc cctcgcgcac tctcacgacg
     9061 gcaaagctgg ccgccgtgtc aaaagagcgc ggtttgcccc tagattggtg gtattccgat
     9121 agcgagcggg aagcggagcg tgccgaaatc cttgcaggcc gccgcgacac cgacctctgg
     9181 gttttcggct atgggtcctt gatgtgggat ccagcgttcc gctttgcgga ggtacggcgc
     9241 gcccgcatat cgggctatgc gcgccgcttt attctcaagg acatctatgg tggcagaggc
     9301 acagtcgatg cgcctggcct catggtcgct cttgacaagg ggccggcgtg cgacggactc
     9361 gcattcagga tttcccgcga aaacatcgaa gaggaaacgg aagctctttg gcggcgtgaa
     9421 agaatcgggc ccgcctacac acctgtcttt gtcgaagccg tcgcagcgga cagtcgcatg
     9481 atggcgctca cgttcgtagc ggaccacgag gctgacctga ttgatgcgag ccttacccgc
     9541 gcgcagcaga tagagtattg cgcgagcggc accggcttta tggggtcgag cctggactat
     9601 ctgaagaata tcaacaggca gttctccgcg ctcggcattc aggacgatga ggtgtccgag
     9661 ctgctacgcg aagccgaagc ctatatcaat tcatgacgga cgctgccggc ttgctgcgag
     9721 cgacctcgtt gctttgggtc atgaacgacc gatcagtcac gtgcgaagca tatgcgccgt
     9781 ctagccaaag gacagtgaag ctgcataaca gcccgctgca cacggactcg cgcgcaagca
     9841 gcgtgctgcg cataggttgt cctgcgcgcg ccatttactg gggacgttgg gcatcgcagt
     9901 acgcgcggcg ttgcagggag tagaggtcaa gtcactttcc gtaatacttg aggagatcag
     9961 ccatgatggt cgcccgttgg agcatcgatg cgaagttcgg atacaaacag aatgcgatcg
    10021 acctgatgca gcgttggtta cgggagattg cgccacaagt cgggtttagt gccgaaaaga
    10081 cgcgcttgct aacgggctcg atcggcgcac tggaggcaac cattcaaaca gagcatctcg
    10141 tcaaggacct caacgaactc aaccaggcgt gggacaaatt ggcgacagtt gcagcgcata
    10201 ggcaatggag caaggatctc gagccgcatg tggtttccgg cacgagccgc tgggaaatat
    10261 accgcgtctt gtagttgcgc ggcgaacggc aagagctcga gacagcaaag gtactggctc
    10321 gcggcggaga catggccaag gcgctcgcgg tgacgcccta tgctctgggg atgacgggca
    10381 tgaccgtggt cgaacagagt gcagggaaag tgaaagcgct gaacctgaac ggcattgctc
    10441 cggcagaaga cgatgtgagg agcggccgct acttcttggc gcgtgacttt ctattcgtta
    10501 tcaaaggaga gccgacaggc ccggtgaaca gattcctcga tcttgtcatg agctcgtgca
    10561 tggcctatcc gtatttcgtg atgtggttcg gcggtcgaca gtcctatcgc cgccacgcac
    10621 ggcggttcgc accgcaatgt ccgattctct ttatccatgg ccgccgcaag cctagccggt
    10681 ttcactcgga ggcgcgggct gatgaattgc tgacgcgcaa aggcaaccag gtgctggagt
    10741 tcgataccgg ccactgggtg atgtccgagc agcctgagcg cttcaatcaa gtggtaagca
    10801 gttgggtctc acaatagcgt ggcgcggcgt gcgcgcgggc agaagagcgc agcccaagcc
    10861 cttcgttcga cgccgacgcg ccgcatgcgg cgcgcgggtg aacgacacgt taggcgtcca
    10921 aagccaccca tccgtttgga ggcggagatg aacatccagg agttcatgca gggttacaag
    10981 gtggcctggg aggcacgcga tccggccatg ttcgctgcgc ttttccacgc tgacgggaag
    11041 taccacaaca cgccgttcca ggttcaaaca ggaaagccgg aacttgccga atactggagg
    11101 cgggtgcaac tgcaggagga ggtgcaggtc agcttcgatg tcctcgcgag ccaaggtgac
    11161 atgggcattg cgcactggca cgttacgtat cgggtggggt cggaggaact gttccaggtc
    11221 tgggcgcgtt caacgggaac gagcctgccg gccaggaagc cgggcgaccc cttgccgcgc
    11281 atggtgctcg atggcgtgct tcaggcaagg tttgcccagg ggctgtgtac ggaggcgcgt
    11341 atctggtggc acagcctgcc gcagcccgcg tgagccgcgc ggcgccgcct gacacctcgt
    11401 cggagccgac gcacagcttt ccaatgcggc gcgtcgatca gctgtttctc ggtgtcgccg
    11461 gggcagtctt cctggggctc ggctctttca acctgctctt tcctgccgcg ggcatggcgg
    11521 gtttcgaaat gcaggtcacc aacgtcagcg cgctgaacga ggcacgcgcc aattacggtg
    11581 gctggcgcgc gccggcgcca ggggctaagc tcgcgcgatg acccgcatcg cgctgctcgc
    11641 cctcgcgctc gccgccgcgc ccgccgcggc cgaggagttc gccttcgccg tcaccgcgcc
    11701 ggccttcaag gtgacgatcc cgaacctgcc ggcgctcgag atggcgccgc acccgatgca
    11761 ggccacgcat ccgcacctgc gcttcctcgg cacgaagggc gcctacaccg tctcggtgat
    11821 cacggcgcgc gccgctgccg gcatgacgcc gctggaatgc gccggcgcga tcctgcgcag
    11881 cctcagcgag cgcccgaacg tgccgccggc cgcggagctc caccgcgccc gcctcgatga
    11941 ccacacctac gccgtcatgt acgcagcgca gctgcccgga ttcctgcagc tgaacgcgca
    12001 cttgctctcg gcggcggccg gcacgcactg catcgaggtg cacgcggcca agatcgccac
    12061 ctcgctagag gacatcgacc cctggttcaa gtccttgggc gaggcgcgca tcgcgcccga
    12121 gtgactattt cttcagctcg atcatcagca cgcgcgtctc ggtgtcgccg acgttggcgc
    12181 cgatatgcgt ctgctcctcc gaccacatca cctcgccgcc cttgaactcg cgctgcagca
    12241 ccttgccgtt gggcagcgtg atgctgcgct tgaaggggct cagggcgtag agcacgaacg
    12301 ccgggtgccg gtgctggtgc gtcttctcgc ccgggcggtc gcggtactcc agcacgcgca
    12361 cgcggtcgtt ctccagcacc accttgtact tgtcgccgtc ggtctgcacc gggtcctgcg
    12421 ccagcgcgct gcccgctccc agcgacatcg ttgcgaacgc cgcggccgcg gccaaatagc
    12481 ggattgccat ggaaactctc cttgggttgg gcaaagcgcc gtactatggc cgtcgccccc
    12541 ggcgcaagtc ccgaccgatg gcctaaggtt gccctaagga gaagcgaaaa tgcacccacg
    12601 cagcccgcct tcgttcaccg tgggcgagtg gcgcgcggac ccgggcctga actgccttgc
    12661 gcgccccggc cacgcgccgc ggcggctcga gcccaaggtc atggaggtgc tcgggtacct
    12721 cgccgcgcac gccggggagg tggtgcctcg ccaggagctg ctccaggcgc tgtggccggg
    12781 cgtcgtggtg ggcgacgagt cgctcacgca gacggtgatc aagctgcgca aggcgctcgg
    12841 cgacgcgtcg cgcgagccac gctacatcga gaccatcgcc aagcgcggct accggctcat
    12901 cgccgcggtg ggccac
//
LOCUS       DBNHKE010000158        10648 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_292069, whole genome shotgun sequence.
ACCESSION   DBNHKE010000158 DBNHKE010000000
VERSION     DBNHKE010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_292069"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="AC16O9_10540"
     CDS             complement(<1..169)
                     /locus_tag="AC16O9_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HIR6517092.1"
                     /translation="MSSASEGLFGAPAPAAPLAERLRPRAIDEVLGQRHLLGPGRPLR
                     LAFESGKPHSMI"
     gene            complement(153..758)
                     /gene="lolA"
                     /locus_tag="AC16O9_10545"
     CDS             complement(153..758)
                     /gene="lolA"
                     /locus_tag="AC16O9_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR6517093.1"
                     /translation="MRGLLLALALAPAVALASGTERFRAFLSGTESARAAFEQKVYDR
                     ERRLVQESKGTFSFQRPGRFRWATTQPYEQLIVGDGERVWIYDPDLKQVTVRALGRAL
                     GATPAALLAGSADVERAFELADAGRIAGLDWLEARPRDKDASFVRIRLGMGPAGVEAM
                     ELHDHFGQTTLLRFDQVVRNPPLDPGEFRFVPPKGADVLGQ"
     gene            complement(759..3038)
                     /locus_tag="AC16O9_10550"
     CDS             complement(759..3038)
                     /locus_tag="AC16O9_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6517094.1"
                     /translation="MAAAQKTTAAPLPAKLGNLLREARWLVLAAVAGYLLLIFLTFDR
                     GDPGWSHSAGGAAATHNAGGRAGAWLADLLLYLFGVSAYWWVALCLVGVAWGYRALER
                     SALVDRRPLAIALGGFALLLLASASLEALRLHSIAAELPLAPGGLLGEAIGGAAAGVL
                     GFTGATLLLLTLVAVGFSLFTGVSWLAVAELTGALLERAAELARHAWESRTDRRIGSL
                     AREERDLQVEAERKREEEHPPLVIAPPPPEIRKSERVHKEKQAPLFESLPDTPLPPVK
                     LLDEGGHDGETLSIESLEYTSRLIEKKLSDFGVSAKVLAAYPGPVITRYEVEPAVGVK
                     GSQIVNLVKDLARALSVVSIRVVETIPGKSCMGLEIPNPHRQTVRLSEILGSEVYADM
                     HSPLALALGKDIAGNPVVTDLQRMPHLLVAGTTGSGKSVALNAMILSLLYKAEPKQVR
                     LILVDPKMLELSVYQDIPHLLAPVVTDMKQAANALMWCVAEMERRYKLMSWVGVRNLS
                     GYNHKVADAEKTGKPLEDPATLDSGAPQPLAQLPYIVVVIDELADLMMVVGKKVEELV
                     ARLAQKARASGIHLIVATQRPSVDVITGLIKANIPTRIAFQVASRVDSRTILDQQGAE
                     ALLGAGDMLYLPAGTGLAQRVHGAFVGDHEVHKVVDHLKSLASPEYIEDVLDPGAGDG
                     GGYEGANGEGGEKDALYDQAVEIVLRTRRPSISLVQRHLRIGYNRAARLIEDMERAGL
                     VSAMQSNGNREVLVPAKAE"
     gene            complement(3085..3753)
                     /locus_tag="AC16O9_10555"
     CDS             complement(3085..3753)
                     /locus_tag="AC16O9_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6517095.1"
                     /translation="MAPPISPAVLHTVPLFASVPEEQLRALATMLTRRSTTRGSIIMA
                     AGDPTDSLYIILSGRLKVMMSDADGKEVILSILGPGEFFGEMGLIDDNPRSASVVAIE
                     PCELLSITRRDFRKCMADNAEIAMAVMRGLVRRLREADRKIGSLALLDVYGRVARLLL
                     DMSETVDGQKMVTKRLPKQDIAKMIGASREMVSRVMKDLQTGGFIEMRGSTILLRDNI
                     VLPE"
     gene            3835..4785
                     /gene="trxB"
                     /locus_tag="AC16O9_10560"
     CDS             3835..4785
                     /gene="trxB"
                     /locus_tag="AC16O9_10560"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR6517096.1"
                     /translation="MTSRHHRLVILGSGPAGYTAAVYAARANLKPVLITGLAHGGQLM
                     TTTDVDNWPADAEGVQGPDLMARFQKHAERFETEVVFDHIQKVQLGVRPFVLQGDAGT
                     YTCDALIIATGASAKYLGLPSEQSYMGKGVSACATCDGFFYKGQEVAVIGGGNTAVEE
                     ALYLANICSKVTLVHRRDKFRAEKIMVDKLMKRVADGKIVLELDATLDEVLGDKSGVT
                     GIRIRHVGNGGAKELAVKGVFIAIGHTPNTQLFDGQLEMANGYIITQAGRDGGFTATS
                     VPGVFAAGDVQDHVYRQAVTSAGTGCMAALDAEKFLDQAA"
     gene            4794..5327
                     /locus_tag="AC16O9_10565"
     CDS             4794..5327
                     /locus_tag="AC16O9_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR6517097.1"
                     /translation="MAHDDDRDAFERAVRGVKPLARARRVDLARPAPAPVPAQTHRDE
                     RAALAESLRGPLSAEEALESGVELTYLREGVSRLALRRLRRGHWVVQASLDLHGMNRV
                     EAAVQVAQFVRASAARGVRCVRIVHGKGLGSRHREPVLKAKLRAWLVPRDEVLAFCQA
                     PAAEGGAGALLVLLKAR"
     gene            complement(5324..5662)
                     /locus_tag="AC16O9_10570"
     CDS             complement(5324..5662)
                     /locus_tag="AC16O9_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6517098.1"
                     /translation="MKKIEAIVKPFKLDEVREALSEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVPEKQLEPAIEAIVKSARTGKIGDGKIFVTTVEHVLRI
                     RTGETNESAI"
     gene            complement(5695..7326)
                     /locus_tag="AC16O9_10575"
     CDS             complement(5695..7326)
                     /locus_tag="AC16O9_10575"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR6517099.1"
                     /translation="MAERLLIAAAQINCTVGDLSANAARILEYTERARAAGADLVLTP
                     EMSLCGYPAEDLVLRAGFLADCRRELEALAARVRGIPLVVGFPELAADGRRYNAAALI
                     RDGRIAAVARKRELPNYDVFDEKRTFDAGSEPCIVELKGVRFGLAICEDVWFARVPAA
                     AKAAGAQAMLSINASPYHKRKQATRHEVFRGWIRDTGMPVVAANLVGGQDELVFDGAS
                     CVLDARGQVVRQAPFFEEALALFAFDGAAPVPGEVAPEPPLEAELYQALVLGTRDYVG
                     KNGFPGAVLGLSGGVDSALTLAVACDALGAARVHAIMMPSQYTARMSLEDAAEMARRL
                     GVRYDTLPIGPAFDAFRASLAPLFAGRPEDLAEENLQARVRGTLLMALSNKFGSIVLT
                     TGNKSEMAVGYSTLYGDMAGGFAVIKDLSKTLVWRLARYRNSLAEVIPENIITRAPSA
                     ELRANQTDQDSLPPYEVLDGIVEAYMEQNRSPQEIVAQGFAPADVRRVVGLIRRSEYK
                     RRQAPPGVRLTLRGFGKDWRYPITSRYRDEDGVSL"
     gene            7350..8471
                     /locus_tag="AC16O9_10580"
     CDS             7350..8471
                     /locus_tag="AC16O9_10580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6517100.1"
                     /translation="MATNESLRVADSLAAIPAAQWNALAGSNPFLRHEFLAALTDSGC
                     ASARTGWQPQFLLLERAGVLAGALPLYVKSHSRGEYVFDWAWADAYAQHGLEYYPKLV
                     CAVPFTPVRGPRLLAADVAARDALAAAALAAARDFSSLHVLFAPGAEAHALQAAGMML
                     RRTVQFHWANAGYADFEAFLARLSHRRRKTIRQERRRVHEAGVRLRWLRGTEIGEREW
                     AFFHRCYVRTYALHGMPPYLNLEFFLRVAAAMPENFLLVLAERRGAPIAAALDVVAGD
                     TIYGRYWGAVEHVPLLHFEACYYQAIEYAIAHGLARFEGGAQGEHKLFRGLLPAESLS
                     AHWLAHRRFARAVEDYLEREARGIARYVNELNEHTPFKV"
     gene            8492..9238
                     /locus_tag="AC16O9_10585"
     CDS             8492..9238
                     /locus_tag="AC16O9_10585"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR6517101.1"
                     /translation="MRLKDKVAIVTGGGSGFGAGIAERFAQEGAKVLVNDINAEGGAR
                     VAKKVGGEFVQADVAKGADWAKLVQAAAAKFGRLDIVVNNAGWTHRRKPYLEVTEAEF
                     DRVYAINVKSIYLSALHALPVMKKQGGGCFVNIASTAGLRPRPGLTVYNSSKGAVILM
                     SKSMAAEFGPDKVRVNCVNPVFSPETALSAEFAGGAVTEEAKKAFLATIPLGRFSTPL
                     DVANACLYLASDEASMISGSCIEVDGARCV"
     gene            complement(9235..10638)
                     /locus_tag="AC16O9_10590"
     CDS             complement(9235..10638)
                     /locus_tag="AC16O9_10590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6517102.1"
                     /translation="MADTSFDQLGLLAPLLQAVRDQGYEHPTPVQEQAIPLVLAGRDL
                     MAGAQTGTGKTAAFALPILQRLAPLASSSASPARHPVRALVLTPTRELAIQVEQSFRD
                     YGKHLPLRSTVVYGGADMDAQIRELRRGVEVLVATPGRLLDHVQSKTVMLNQVGILTL
                     DEADRMLDMGFLPDIRRIIALLPAVRQNLLFSATFPDEIRGLVKSLLKNPAEVQVAAR
                     NAVAELVTHVVHPVAREKKRELLSYLIQTRNLQQVLVFCGTRIGANRLAHQLRKDRIH
                     ADAIHGDKSQAERLVALENFKAGKTAVLVATDVASRGLDIEGLPQVINFDIPHSPEDY
                     VHRIGRTGRAGSAGEAISLVAPQDFDALAAIERLIKRKLERTLVPGFQPDGGTVATLV
                     SRNGSREGRRGPRREEGSRTAVREPKAPRPIGDPIFTKPYEPAPRAEDSAPRAEEPQR
                     AKRRQPQVAALLGGMKR"
BASE COUNT         1551 a         3768 c         3751 g         1578 t
ORIGIN      
        1 ggatcatcga gtgcggcttg ccggattcga aggcaagccg cagcggccgg cccggcccga
       61 gcaggtggcg ctggccgagc acctcgtcga tggcacgcgg ccgcaggcgc tcggcgagcg
      121 gcgccgccgg cgccggcgcg ccgaacaggc cctcactggc cgaggacatc cgcgcccttg
      181 ggcggcacga agcggaactc gcccgggtcg agcggcggat tgcgcaccac ctggtcgaag
      241 cgcagcagcg tggtctggcc gaagtgatcg tgcagctcca ttgcctctac gcccgccggc
      301 cccatgccca gacggatgcg cacgaaactc gcgtccttgt cgcgcgggcg cgcttccagc
      361 cagtccaggc ccgcgatgcg ccccgcgtcg gcaagctcga aggcgcgctc gacgtcggcc
      421 gagcccgcga gcagcgccgc gggcgtggcg ccgagcgcgc gcccgagcgc gcgcaccgtc
      481 acctgcttca ggtccgggtc gtagatccag acgcgctcgc cgtcgccgac gatcagctgc
      541 tcgtagggct gcgtcgtcgc ccagcggaag cgcccggggc gctggaacga gaacgtgccc
      601 ttggattcct gcaccaggcg ccgctcgcgg tcgtacacct tctgctcgaa cgccgcgcgc
      661 gcgctctcgg ttccgctcag gaacgcgcgg aagcgctcgg tgcccgaggc aagcgccacc
      721 gcgggtgcca gcgccagcgc cagcagcagg ccgcgcacct actccgcctt cgccggcacc
      781 agcacctcgc ggttgccgtt ggactgcatt gccgacacga ggcccgcacg ctccatgtcc
      841 tcgatcaggc gcgccgcgcg gttgtagccg atgcgcaggt ggcgctgcac gagcgagatc
      901 gacggccgac gcgtgcgcag cacgatctcc accgcctggt cgtagagcgc gtccttctcc
      961 ccgccttcgc cgttcgcgcc ctcgtagccg ccgccatcgc ccgcacccgg atcgagcacg
     1021 tcttcgatgt actcgggcga ggccagcgac ttcaggtgat ccaccacctt gtgcacctcg
     1081 tgatcgccga cgaacgcgcc gtgcacgcgc tgcgcgaggc cggtgcccgc ggggaggtac
     1141 agcatgtcgc cggcgccgag cagcgcctcc gcgccctgct gatccaggat ggtgcgcgag
     1201 tcgacgcggc tggccacctg gaaggcgatg cgcgtcggga tgttggcctt gatgagcccg
     1261 gtgatcacgt ccaccgaggg ccgctgcgtg gcgacgatga ggtggatgcc cgaggcgcgc
     1321 gccttctgcg cgaggcgcgc caccagttcc tccaccttct tgcccaccac catcatcagg
     1381 tcggcaagct cgtcgatgac cacgacgatg tagggcagct gcgcgagcgg ctgcggcgcg
     1441 cccgagtcga gcgtggccgg gtcttccagc ggcttgccgg tcttctccgc gtcggccacc
     1501 ttgtggttgt agcccgacag attgcgcacg cccacccagc tcatcagctt gtagcgccgc
     1561 tccatctccg cgacgcacca catgagcgcg ttggccgcct gcttcatgtc ggtgaccacc
     1621 ggcgcgagca ggtgcgggat gtcctggtac accgagagct cgagcatctt cgggtcgacc
     1681 aggatcaggc gcacctgttt gggctcggcc ttgtacagca gcgacaggat catcgcgttc
     1741 agcgccaccg acttgcccga gccggtggtg ccggccacca gcaggtgcgg catccgctgc
     1801 aggtcggtca ccacggggtt gccggcgatg tccttgccga gcgcgagcgc gagcggcgag
     1861 tgcatgtcgg cgtacacctc ggagccgagg atctccgaca ggcgcacggt ctggcggtgc
     1921 gggttcggga tctcgaggcc catgcacgac ttgccgggga tggtctcgac cacgcggatc
     1981 gacaccaccg agagggcccg cgccaggtcc ttcaccaggt tgacgatctg gctgcccttc
     2041 acgccgacag cgggctcgac ctcgtagcgc gtgatcacgg ggccgggata ggcggccagc
     2101 accttcgccg agacgccgaa gtcggaaagc ttcttctcga tcagccgcga ggtgtactcc
     2161 agcgactcga tgctgagcgt ctcgccgtcg tggccgccct cgtcgagcag cttcaccggc
     2221 ggcagcggcg tgtcgggcag cgactcgaac agcggcgcct gcttctcctt gtgcacgcgc
     2281 tcggacttgc ggatctccgg cggcggcggc gcgatgacca gcggcggatg ctcctcctcg
     2341 cgcttccttt cggcctcgac ctgcaaatcg cgttcctcgc gcgccaggct gccgattctc
     2401 cggtcggtgc ggctctccca ggcatggcgc gccagctccg cggcacgctc gagcagcgcg
     2461 ccggtgagct cggccaccgc cagccacgac acgccggtaa acaggctgaa gcccacggcg
     2521 acgagggtaa gcagcagcag cgtggcgccg gtgaagccga gcacgcccgc ggcggcgccg
     2581 ccgatggcct cgccgagcag cccccccggc gcgagcggca actctgcggc gatggaatgc
     2641 aggcgcaggg cttccagcga cgcgctcgcg agcagcagca gcgcgaagcc accgagcgcg
     2701 atggcgagcg gccgccggtc gacgagcgcc gagcgctcga gcgcgcggta gccccaggcg
     2761 acgcccacca ggcacaacgc cacccaccag tacgcggaca cgccgaagag atacagcagc
     2821 aggtcggcca gccaggcgcc ggcgcggccg ccggcgttat gcgtcgccgc cgcgccgccg
     2881 gcgctgtgcg accagcccgg gtcgccgcgg tcgaaggtga ggaagatgag cagcaggtag
     2941 cccgcaacgg ccgccagcac cagccaccgg gcctcgcgca acaggttgcc gagcttcgcg
     3001 ggaaggggcg ctgctgtggt tttctgtgct gctgccatcg cgcacccgta ggggtccgcc
     3061 gattctaccg caggggcgtg accgtcactc cggcaggacg atgttgtcgc gcagcaggat
     3121 ggtcgagccg cgcatctcga tgaagccgcc ggtctgcagg tccttcatca cgcggctcac
     3181 catctcgcgc gaggcgccga tcatcttggc gatgtcctgc ttgggcaggc gcttggtgac
     3241 catcttctgc ccgtcgaccg tctcggacat gtcgagcagc agccgcgcga cgcgcccgta
     3301 cacgtcgagc agcgccaggc tgccgatctt gcggtcggcc tcgcgcaggc gccgcaccag
     3361 cccgcgcatc actgccatcg cgatctcggc gttgtcggcc atgcacttcc tgaagtcgcg
     3421 ccgcgtgatc gacagcagct cgcagggctc gatcgccacc acgctcgccg agcgcgggtt
     3481 gtcgtcgatc aggcccatct cgccgaagaa ttcgcccggc ccgaggatgc tgaggatgac
     3541 ttccttgccg tcggcatcgc tcatcatcac cttcagtcgg cccgaaagga tgatgtagag
     3601 cgagtcggtg gggtcgcccg cggccatgat gatcgagccg cgcgtcgtac tgcggcgcgt
     3661 gagcatggtg gcaagcgcgc gcagctgctc ctccggcacg ctcgcaaaga gcggcaccgt
     3721 gtgcagcacg gccggtgaga tcgggggcgc catgggccag ggtccgtcgg gatatggtag
     3781 caaactggcc gcgccttgtt atttataatg cggtgcgacc ggtttgcagc ctacatgacc
     3841 tcccgccatc accgcctcgt catcctcggt tccggccccg ccggctacac cgccgcggtg
     3901 tacgcggcac gcgccaacct gaagccggtg ctgatcaccg gcctcgccca cggcgggcag
     3961 ctgatgacca ccaccgacgt ggacaactgg cccgccgacg ccgagggggt gcagggcccc
     4021 gacctgatgg cgcgcttcca gaagcacgcc gagcgcttcg agaccgaggt ggtgttcgac
     4081 cacatccaga aggtgcagct cggcgtgcgc ccgttcgtgc tccagggcga cgccggcacc
     4141 tacacctgcg acgcgctcat catcgccacg ggcgcgtcgg cgaagtacct cggcctgccc
     4201 tccgagcaaa gctacatggg caagggggta tccgcctgcg ccacctgcga cggcttcttc
     4261 tacaagggcc aggaggttgc ggtgatcggc ggcggcaaca ccgccgtgga ggaggcgctg
     4321 tacctcgcca acatctgcag caaggtgacg ctggtgcacc ggcgcgacaa gttccgcgcc
     4381 gaaaagatca tggtcgacaa gctgatgaag cgcgtggccg acggcaagat cgtgctcgag
     4441 ctcgacgcca cgctcgacga ggtcctgggc gacaagagcg gcgtgaccgg aatccgcatc
     4501 cggcacgtcg gcaacggtgg ggcgaaggag cttgcggtga agggcgtgtt catcgccatc
     4561 ggccacacgc ccaatacgca gctcttcgac ggccagctcg agatggcgaa cggctacatc
     4621 atcacccagg ccgggcgcga cggcggcttc acggcgacca gcgtgcccgg ggtattcgcc
     4681 gcgggcgacg tgcaggacca cgtctaccgc caggcggtga ccagcgccgg caccggctgc
     4741 atggcggcgc tcgacgccga gaaattcctc gaccaggcgg cctgaggccg gcgttggcgc
     4801 acgacgacga ccgcgacgcc ttcgagcgtg ccgtgcgggg cgtcaagccg ctggcgcggg
     4861 cccggcgcgt cgacctcgcc cggccggcgc ccgcgccggt tcccgcgcag acgcatcgcg
     4921 acgagcgcgc cgcgctcgcc gagagcctgc gcggcccgct ctcggccgag gaagcgctcg
     4981 agtccggcgt cgagctgacc tacctgcgcg agggcgtgtc gcgcctcgca ctgcggcggc
     5041 tgcgccgcgg gcactgggtg gtacaggcga gcctcgacct gcacggcatg aaccgcgtgg
     5101 aagcggcggt gcaggtggcg cagttcgtgc gcgcgtccgc ggcccggggc gtgcgctgcg
     5161 tgcgcatcgt gcacggcaag ggcctgggat cgaggcaccg cgagccggtg ctgaaggcga
     5221 agctgcgcgc atggctcgtg ccgcgcgacg aggtgctggc gttctgccag gcgccggccg
     5281 ccgaaggcgg cgccggcgcg ctgctggtgc tgctgaaggc gcgctagatc gcggactcgt
     5341 tggtctcgcc ggtgcggatg cgcagcacgt gctcgaccgt ggtgacgaaa atcttgccgt
     5401 cgccgatctt gccggtgcgc gccgacttga cgatggcttc gatcgccggc tcgagctgct
     5461 tctccgggac caccacttcg atcttcacct tcggcaggaa gtcgaccacg tactcggcgc
     5521 cgcggtagag ctcggtatgg cccttctggc ggccgaagcc cttcacttcc gtgacggtga
     5581 ggccggtgac gccgacctcg gagagcgcct cgcgcacctc gtccagcttg aacggcttca
     5641 cgatggcttc gatcttcttc atgctcatgc ctgatcctcc cgggatgccc ccaattatag
     5701 cgacacgccg tcctcgtcgc ggtagcgcga ggtgatcggg tagcgccagt ccttgccgaa
     5761 gccgcgcagg gtgaggcgca ccccgggcgg cgcctggcgg cgcttgtact cgctgcggcg
     5821 gatcaggccg accacccggc gcacgtccgc gggcgcgaag ccctgggcaa cgatctcctg
     5881 cggcgagcgg ttctgctcca tgtaggcctc gacgatgccg tccagcactt cgtagggcgg
     5941 caggctgtcc tggtcggtct ggttggcgcg cagctcggcc gagggcgcgc gcgtgatgat
     6001 gttctcgggg atcacctcgg ccaaagagtt tcggtagcgc gccaggcgcc ataccagcgt
     6061 cttggacagg tccttgatca ccgcgaagcc gccggccatg tcgccgtaca gggtcgagta
     6121 gccgaccgcc atctccgact tgttgccggt ggtgagcacg atcgagccga acttgttgga
     6181 caaggccatg agcagcgtgc cgcgcacccg cgcctggagg ttttcctcgg cgagatcctc
     6241 gggccggcct gcgaacaatg gcgcgaggct ggcgcgaaag gcgtcgaacg ccgggccgat
     6301 ggggagggtg tcgtagcgca cgcccaggcg ccgcgccatc tccgcggcgt cctcgaggct
     6361 catgcgcgcg gtgtactgcg acggcatcat gatcgcatgc acgcgcgcgg cgccgagcgc
     6421 gtcgcaggcg acggcgagcg tgagcgccga atcgacgccg cccgacaggc cgagcaccgc
     6481 accgggaaag ccgttcttgc cgacgtagtc gcgcgtgccg agcaccagcg cctgatagag
     6541 ctcggcctcg agcggcggct ccggggccac ctcgccgggc acgggcgcgg cgccgtcgaa
     6601 cgcgaacagc gccagcgctt cctcgaagaa cggcgcctgg cgcaccacct gcccgcgcgc
     6661 gtcgagcaca cacgaggcgc cgtcgaacac cagctcgtcc tggccgccga caaggttcgc
     6721 cgccaccacc ggcatcccgg tgtcgcggat ccagccgcgg aacacctcgt ggcgcgtcgc
     6781 ctgcttgcgc ttgtggtagg gcgaagcgtt gattgacagc atggcctgcg cgccggccgc
     6841 cttggccgcg gcgggcacgc gggcgaacca cacgtcctcg cagatggcga gcccgaagcg
     6901 cacgcccttc agctccacga tgcatggctc ggacccggcg tcgaaggtgc gcttctcgtc
     6961 gaacacgtcg tagttgggca gctcgcgctt tcgcgccacc gcggcgatgc gcccgtcgcg
     7021 aatcagcgcc gcggcgttgt agcgccggcc gtccgcggcg agctcgggaa agccgaccac
     7081 cagcgggatg ccgcgcacgc gcgcagccag cgcctcgagc tcgcgccggc agtcggcgag
     7141 aaagcccgcg cgcagcacca ggtcctcggc cggatagccg cacagcgaca tctccggcgt
     7201 gagcaccaga tcggcgccgg ccgcgcgcgc gcgctccgtg tactccagga tgcgcgcggc
     7261 gttggcggaa aggtcgccca cggtgcagtt gatctgcgcc gcggcgatca gcaggcgttc
     7321 ggccatcgcg gggattctat aatgcggcca tggcgacgaa cgaatcgctg cgcgtcgccg
     7381 actcgctcgc cgcaatcccc gcggcgcaat ggaacgccct cgccgggtcg aatccgttcc
     7441 tgcgccacga gttcctcgcc gcgctgaccg acagcggctg cgcgagcgcg cgcaccggct
     7501 ggcagccgca gtttctgctg ctcgagcgcg cgggcgtgct cgccggcgcc ctgcccctgt
     7561 acgtgaagtc gcattcgcgc ggcgagtacg tgttcgactg ggcctgggcc gacgcctatg
     7621 cgcagcacgg gctcgagtac tacccgaagc tcgtgtgcgc ggtgccgttc acgccggtgc
     7681 gcgggccgcg cctgctcgcg gccgacgtgg cggcgcgcga cgcgctcgcc gccgccgcgc
     7741 tcgccgcggc gcgcgacttc tcgtcgctgc acgtgctgtt cgcgccgggt gccgaggcgc
     7801 acgcgctgca ggcggcgggg atgatgctcc ggcgcacggt gcagttccac tgggccaatg
     7861 ccggctacgc cgacttcgag gcgttcctcg cgcggctgtc gcaccggcgg cgcaagacca
     7921 tccgccagga acggcgccgc gtgcacgagg cgggcgtgcg gctgcgctgg ctgcgcggca
     7981 ccgagatcgg cgagcgcgag tgggcgttct tccaccgctg ctacgtcagg acctacgcgc
     8041 tgcacggcat gccgccgtac ctcaacctgg agttcttcct gcgcgtggcg gcggccatgc
     8101 cggagaactt cctcctcgtg ctcgccgagc ggcgcggcgc gccgatcgcc gcggcgctcg
     8161 acgtggtggc gggcgacacg atctacggcc gctactgggg cgcggtggaa cacgtgccgc
     8221 tgctgcactt cgaggcctgc tactaccagg cgatcgagta cgccatcgcg cacggcctgg
     8281 cgcgcttcga aggcggcgcc cagggcgagc acaagctgtt ccgggggctg ctgcccgcgg
     8341 agtcgctctc ggcgcactgg ctggcgcacc ggcgcttcgc gcgcgccgtc gaggactacc
     8401 tcgagcgcga ggcgcgcggc atcgcgcgct acgtgaacga gttgaacgag cacacgccct
     8461 tcaaagtatg attcccggca aaggaggagc catgagactg aaggacaagg ttgccatcgt
     8521 caccggtggc gggtcggggt tcggcgccgg catcgccgag cgcttcgccc aggaaggcgc
     8581 gaaggtgctg gtcaacgaca tcaacgccga gggcggcgcg cgcgtcgcga agaaggtcgg
     8641 cggcgagttc gtccaggccg acgtggcgaa gggcgcggac tgggcgaagc tggtgcaggc
     8701 cgccgccgcc aagttcggcc ggctggacat cgtggtcaac aacgccggct ggacccaccg
     8761 gcgcaagccc tacctcgagg tcaccgaggc cgaattcgac agggtctacg ccatcaacgt
     8821 gaagagcatc tacctctcgg cgctgcacgc gctgccggtg atgaagaagc agggtggcgg
     8881 ctgcttcgtc aacatcgcct cgacggcggg cctgcgcccg cgcccggggc tcaccgtgta
     8941 caacagctcc aagggcgccg tgatcctgat gtccaagtcg atggccgccg agttcggccc
     9001 cgacaaggtg cgcgtgaatt gcgtcaaccc cgtgttcagc cccgagaccg cgctgtccgc
     9061 ggagttcgcc ggcggcgcgg tcaccgaaga ggcgaagaag gcgttcctcg ccacgatccc
     9121 gctggggcgc ttctccaccc cgctcgacgt cgcgaacgcg tgcctgtatc tcgcctcgga
     9181 cgaggcgtcg atgatctcgg gcagctgtat cgaggtggac ggggcgcgct gcgtctagcg
     9241 cttcatcccg cccaggaggg cggcgacctg cggctggcgg cgcttggcgc gctgcggctc
     9301 ttcggcccgt ggcgctgaat cttccgcgcg cggcgcgggc tcgtatggct tggtgaagat
     9361 cgggtcgccg atcggccgcg gcgccttcgg ctcgcgcacg gcggtccggg acccttcctc
     9421 gcgacgcggg ccgcggcgcc cttcgcggct gccgttgcgg ctcaccagcg tggccaccgt
     9481 gccgccgtcg ggctggaagc cgggaaccag cgtgcgttcg agcttcctct tgatcagccg
     9541 ctcgatcgcc gccaaggcgt cgaagtcctg cggcgccacc agcgagatcg cctcgcccgc
     9601 gctgccggcg cggccggtgc ggccgatgcg gtgcacgtag tcctcgggcg agtgcgggat
     9661 gtcgaaattg atcacctgcg gcaggccctc gatgtccagg ccgcgcgagg cgacgtcggt
     9721 ggccaccagc accgcggtct tgccggcctt gaagttctcc agcgccacca ggcgctcggc
     9781 ctgcgacttg tcgccgtgga tggcgtcggc gtggatgcgg tccttgcgga gctggtgcgc
     9841 gaggcggttg gcgccgatgc gcgtgccgca gaagaccagc acctgctgca ggttgcgcgt
     9901 ctggatcagg taggaaagca gctcgcgctt cttttcgcgc gccaccgggt gcaccacgtg
     9961 cgtcaccagc tcggccaccg cgttcctcgc cgccacctgc acctcggcgg gattcttcag
    10021 cagcgacttc accaggccgc ggatttcgtc ggggaaggtc gcggaaaaca gcaggttctg
    10081 ccgtaccgcc ggcagcagcg cgatgatgcg gcggatgtcg ggcaggaacc ccatgtccag
    10141 catgcggtcg gcctcgtcca gggtgaggat gccgacctgg ttcagcatca cggtcttgct
    10201 ctgcacgtgg tcgagcagcc gtccgggggt ggccaccagc acttcgaccc cgcggcgcag
    10261 ctcgcggatc tgcgcgtcca tgtcggcgcc gccgtagacg acggtgctcc tcagcggcag
    10321 gtgcttgcca tagtcgcgga agctctgctc gacctggatc gcaagttcgc gcgtcggcgt
    10381 gagcaccagc gcgcgcaccg ggtgccgcgc gggcgaggcg ctcgaactcg cgagcggggc
    10441 gagccgctgc aggatcggca gcgcgaacgc cgcggtcttg ccggtgccgg tctgcgcgcc
    10501 tgccatcagg tcccggcccg cgagcaccag cgggatcgcc tgctcttgca ccggcgtggg
    10561 atgctcgtaa ccctgatcgc gcaccgcttg caggaggggc gcaagaagtc cgagctggtc
    10621 gaaggaagta tctgccaaga gcgcgaat
//
LOCUS       DBNHKE010000159        34055 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_609628, whole genome shotgun sequence.
ACCESSION   DBNHKE010000159 DBNHKE010000000
VERSION     DBNHKE010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 34055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_609628"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..640)
                     /locus_tag="AC16O9_10595"
     CDS             complement(2..640)
                     /locus_tag="AC16O9_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR6517103.1"
                     /translation="MAGLAPLGDYLRFVVTLTAVVDPFLAVPFFLTFAASHGEDARKH
                     LARTVAVTVFLVLAVAALVGEALLDLIGASLPAFRVGGGLVLLLMALAMLNAQAGGVR
                     QSKAEAEELEEGELQGVVPLAIPLLAGPGAISTAIIATEGGGAAHQVIIIACIAVVCG
                     VVWLALRAAHQVAARMGTTGMNVATRILGLLLAAMAVQTMAVGLKALFPGLG"
     gene            complement(645..1289)
                     /locus_tag="AC16O9_10600"
     CDS             complement(645..1289)
                     /locus_tag="AC16O9_10600"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6517104.1"
                     /translation="MIRLCGFRISNYHNKVRIALLEKGVAFEEDENVKPSQEGDYLVR
                     SPMGKVPYLEVNGARIRESSAILEYLEDAFPQKPLLPKDPLERARVRELVIFIELHLE
                     LVARRMYGMLAGGKPVSEETRQRVEKDLAKGVRALKAVAKFEPYIAGSELTIADCAAF
                     VHLPLVTLVTRNFLGRDFLEDMPQVKPYLKMLGERPAFAKVNEDRKAASAAARK"
     gene            complement(1286..2056)
                     /locus_tag="AC16O9_10605"
     CDS             complement(1286..2056)
                     /locus_tag="AC16O9_10605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6517105.1"
                     /translation="MDLKGKVAVITGAASGIGRAAALRFAQEGAAGLVLADLNMAALN
                     PVAEEAKGLAVACDVGREADIQRLVRETEERHGRVDVYFSNAGIIRRGGLEASDADWE
                     RNWRIHVMAHVWAARAVVEKMVARGGGYFLVTASAAGLLNIVESATYGVTKHGAVAFA
                     EWLAIAYGRRGLRVSCLCPQAVLTGMYKGEGGSAGVDGALSAEQVAGDIVKAMAEERF
                     LILPHPTVLNYMRNKTADYDRWIGGMQKIYAKHEGEKR"
     gene            complement(2059..3303)
                     /locus_tag="AC16O9_10610"
     CDS             complement(2059..3303)
                     /locus_tag="AC16O9_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517106.1"
                     /translation="MDFEYSPKVQELRKRLIAFMDEHIYPNESAYYAHTRGDKRWTPV
                     PIIEELKPKARKAGLWNLFLPHSKRAPEGLSNLEYAPLCEIMGRVSWASEVFNCSAPD
                     TGNMETIERYGTEEQKKQWLEPLLAGEIRSAFLMTEPAVASSDATNIQMHIKRDGNDY
                     VINGRKWWSSGAGDPRCKIYIVMGKTNPDHASRHSQQSMILVPAATPGIKILRNLNVF
                     GYDDAPHGHMEIEFKDVRVPASNILLGEGRGFEIAQGRLGPGRIHHCMRSIGAAERAL
                     EKMCKRATQRVAFGRTVAEQGVTRERIAEARIMIDSARFLVLHAAWRMDTVGNKVARK
                     EIAMIKVLAPNMAGQVIDWAMQVHGGGGVSDDFGLANMYAHQRTLRFADGPDEVHRNA
                     IAKLEIAEQTSVTEALAGRKRG"
     gene            complement(3313..4383)
                     /locus_tag="AC16O9_10615"
     CDS             complement(3313..4383)
                     /locus_tag="AC16O9_10615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6517107.1"
                     /translation="MFEQFIGVKPVEERHRFDAGRLAQFLRGKIDDWRGELAIEQFKG
                     GQSNPTYRITAGDKRYALRRKPPGKLLPSAHAVEREYKVIRALHGVGFPVAKPYVLCE
                     DESVIGTAFYVMDCVEGRVLWDQALPGMTREARGAIWDELNRVIALLHGVDYKAVGLE
                     DFGKAGDYIARQVSRWSKQYRASELERIDEMDRLIEWLPANIPAGDETSVVHGDYRLD
                     NAIFHPSEPRILAVLDWELSTLGHPLADFAYHCMSWHIAPGKFRGIAGLDLEALGIPT
                     ERQYVERYCRRTGRKGIDPSTWDYYMAYNLFRIAAITQGIAKRVVDGTAASEYAREAG
                     SRTRPLAELGWAQVEKILKRAA"
     gene            complement(4434..4871)
                     /locus_tag="AC16O9_10620"
     CDS             complement(4434..4871)
                     /locus_tag="AC16O9_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6517108.1"
                     /translation="MATERRNVLIVDDHEPTRLLIGRIVTQELGARVTLAGTCEQALR
                     LAEEAPYDVILLDLLMPGIGGYEVLRQLRERGANQATPILVVSVMSTHESRERCRLLG
                     ATAFIAKPVDRELITTMLRSLIGSRGADKAQPENGAARHDNGC"
     gene            complement(4894..6321)
                     /locus_tag="AC16O9_10625"
     CDS             complement(4894..6321)
                     /locus_tag="AC16O9_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6517109.1"
                     /translation="MHTIGNIHIFPQGVGGLMRNSLVFVSFALGAAFVSPALGQSAGV
                     IQFVAGNVQLVQPDGVGRLASKGVPVKVGDTLATAKGAMAQLKMGDGAIVVVVPDSRV
                     TVAEFRYAGVEDGTEKVRYRLEHGGFRAVTGAIGRTYKRNYVIETPIAHMGVRGTDHE
                     TYYFPATGPVSADDAKPGAYNKVNTGKTFIRTGAGEVEIEPNQVGYVASANDVPVILP
                     MVPGFFNRSIAPRNARPAADPAPEAAEVDEVVQQVARSTNGVRLLRTRGPLVTPGTGN
                     GPLVGFVTAVGGGRFGNSGNGVTVLDNGAPSTGGSAPAGTINWTTWQGGVAVVGGQPT
                     AGSTHMISTANRTDLATLSPAVMSATYNYTGGPAFTDQLGNQATLNSMSVGVDFSSQV
                     VTRYDLNATAASGYNWNVNLDTTAPRAIADFTGVGGVRLKGGGGQAGIAHGTFVGTQA
                     ENLITTFGLSGPADRSLSGAAYLTR"
     gene            complement(6379..7707)
                     /locus_tag="AC16O9_10630"
     CDS             complement(6379..7707)
                     /locus_tag="AC16O9_10630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6517110.1"
                     /translation="MLLAVLVAAAVMAGPGRVAAQSEIGDLLARASALSEAGRYAEAY
                     ALLAAQEDTHIGEVDFDYALGRAALNAGRPDRATIAFSRVLAIDSGHAGARIDMGRAY
                     LALENWAQARVVFEALLALDPPPALRAQLLLYLGESRGESRARAAARGYLSVFAGTST
                     NVNQAPGQSQVFVPGLVAVLQLADQNVGKDDGFTGVGGGVEAAMPLRGRVSLIGNAEF
                     LSRWNAHETAFDVGGVAASTGLAWAGERHVVRSQLQMVRNTLGGSTSREVRAFSLDFA
                     ERSPAPGTPGAMFAYLHAGGFRHPPVDLQIFDADFVAAGVGATVPIDRKSTASVVFMA
                     GGDNDRGGNPGGDRRGVGARFAWEHVLGPDLRFSALASVQESRYDGFDSAFLVRRDDR
                     RTDLEGFLRYDLSSKLELRFGVLRSVQESNIEIYEFRRTDWTLTLRRVFN"
     gene            7842..10541
                     /locus_tag="AC16O9_10635"
     CDS             7842..10541
                     /locus_tag="AC16O9_10635"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR6517111.1"
                     /translation="MQSDATLPWLRVLVSAAGGLLLAALFSQSTLFSRLSWWVDDALQ
                     RRFAVALPLDGVAVVDVDEASLQRLQPRLGAWPYPRDAYAQAHRFLVANGARVVAYDI
                     LFAEPRAGDAAFAEALDRRAVLAAAALPYPYARSSQYQEHLARIALSDVQDDAPAAQL
                     AHAWPDLTLPLEQFTQPGKARVGVISTLADDDGVVRRLNVLHLAHGKVLPGFAVAAML
                     PGGPEQPLRVERGQLRLGDRTWPLAADGSVAPRLPANLADLAVIPFFQVLDAAEGVSG
                     TAHVADLVRGRHVFVGSSSTVLGDVALTPAGRFPGLHVNALVAASLQAGEVLRPPRAW
                     LDIALAVLALALPAALARSGVALRPRDFAIALGLGAVLLALAVIALAAARQQSHWLFA
                     ATAGVGALGVAVAAWLFALYQEKQRLFYEKLAALEASRLKSEFLNHMTHELRTPITAI
                     MGFNKFNLYGDDIGRDQRLRHSAIIARNCEHLLALVNNNLDLARMEAGQLKFERTAHE
                     VRALLDDVVATLRVMAHDKGLALEQEVAADMPAALSLDAFRVRQVLINLIGNAIKFTA
                     QGKVVLKASWHSGELRFEIADTGPGIPQESLERIFVPFQRAPGVTAAGTGLGLSITRK
                     LVELLGGSIHARSEVGKGSVFEARIPAPPAQLPASTVSSAPTPMPAKLAGRILLADDN
                     ADLRALVQVQLSDLGFECKAVGDGLEAIEAALAQPYSVVLLDMDMPFMDGYETVRVLR
                     ERGYTAPVIGFTAHQAGAPVERALIEGCDDVISKPASPERLREALAPFAEGALPPQPA
                     AGADAIRVGVDARLRGIVAKFLSNCGRDVQRLQHALDGGDLATTRAIGHALRGAGGSY
                     GFDEITRIGKSIEERSMHGDAASLGPLVAQLQNYLSRVHPEFR"
     gene            complement(10549..11004)
                     /gene="dtd"
                     /locus_tag="AC16O9_10640"
     CDS             complement(10549..11004)
                     /gene="dtd"
                     /locus_tag="AC16O9_10640"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR6517112.1"
                     /translation="MIALVQRVAEARVAAGAEQLGAIGRGVLALVGVERGDTEREAAR
                     LAERVLGYRIFADAEGRMNRSLADIAGALLVVPQFTLAADTSSGTRPGFSTAAAPADG
                     ERLYAVFVRACRERHAEVASGRFGAHMQVSLVNDGPVTFWLRAAPASQA"
     gene            complement(11001..11222)
                     /locus_tag="AC16O9_10645"
     CDS             complement(11001..11222)
                     /locus_tag="AC16O9_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HIR6517113.1"
                     /translation="MSNQPLTALERAQIEARILALETEHHDLDDIIARLALDPAQDQL
                     QLRRLKKKKLLLKDLLARLRDQLIPDIIA"
     gene            11423..12253
                     /locus_tag="AC16O9_10650"
     CDS             11423..12253
                     /locus_tag="AC16O9_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517114.1"
                     /translation="MRTAILQLYQGLYPGTADSTIRGAFDYLVHLYRGGHPEFHACDT
                     QFHDTQHVLEVTLAMARLMEGYERSRSEGAPLSPTLYSVGVVSALFHDVGYLRRRSDR
                     RHAHGAEYTLTHVGRSARFVSAYLRSVGLARYAGAAAAIVHYTGHERPAEAIRIDGAL
                     PRRIGQMLGTADLIAQMADRCFLEKRRDRLYPELVAGGVAGRLIRSAGELVRRTYEFH
                     LRAMRRLDLQLARAYGYAERHFGGQNLYLEALQQNARYALVLANADAAGRLRRAPPAQ
                     "
     gene            complement(12247..13236)
                     /locus_tag="AC16O9_10655"
     CDS             complement(12247..13236)
                     /locus_tag="AC16O9_10655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6517115.1"
                     /translation="MPTICRSLLLALACTAGPALSQDRPDADAEATLSAVVRVQSRIL
                     PDARTLATLGARREGSGVLVREGYVLTIGYLVIEADAIEVTGGDGKSAPATLAAYDHA
                     TGFGLLKLAAPLAGKPLPLGDSAALAERDAAMVVGYGGREALNLVTVVSRRPFSGSWE
                     YLLEAAIFTYPPVHNWSGAALISARGELLGIGSLIVPDAGGAGTPAPGNLFVPIDLLQ
                     PILDDLIAHGGAPGPRRPWLGVYAEEVRGRLFVTRVAREGPADRAGLAGGDLVIGVGS
                     EDVASLADFYRKLWARGAAGVEVPLRVLQGSELKELRVRSIDRLQYFRRKPSY"
     gene            13288..14109
                     /locus_tag="AC16O9_10660"
     CDS             13288..14109
                     /locus_tag="AC16O9_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6517116.1"
                     /translation="MRLVSWNLQWCRGMDGVVDPRRVARTVRELADADLCCFQEVAQG
                     FGALPGSAGEDQVAALAAEFAGYSAHYAWGVDVAGAAGRRRRFGNLILSRLPVGRVLR
                     HGLPWPPDAGVPSMPRVAIEAVVAAPWGQVRVVTTHLEYYSARQRAAQVERLLELNAE
                     AAAHAAAKPAARDDQGPFHPQPRPAAGILTGDFNMTPEDPLVARLGSAYADAWRQAHP
                     GRPHAPTFRLESADEAPYCCDFVFVTPDLASRVRSVRVDAQTRASDHQPVIVELN"
     gene            complement(14113..14640)
                     /locus_tag="AC16O9_10665"
     CDS             complement(14113..14640)
                     /locus_tag="AC16O9_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR6517117.1"
                     /translation="MSGSCADVSIGASMQRRALTGIVLLAVVLGAAIALHLAIAEGDT
                     VFAGKTPGNLGVQDGRLASCGRRLNCVSSQADAGDAQRYVTPIPFKGRAAEAIAAARR
                     AVESMPRARVVREEGNYLHAEFRSKLMGYVDDVEFTFDAGAGVLHVRSASRLGRRDFQ
                     VNRERVEALRARLVR"
     gene            14653..14931
                     /locus_tag="AC16O9_10670"
     CDS             14653..14931
                     /locus_tag="AC16O9_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517118.1"
                     /translation="MEISAHQLAELLIGIARAQHTIIQGVESAMAGTKTQHILPMLQN
                     AAHLRDHPEPTLVDLPVRVLLSTQGRVPPDPAAVARDLERLLAAAPAA"
     gene            complement(14928..15740)
                     /locus_tag="AC16O9_10675"
     CDS             complement(14928..15740)
                     /locus_tag="AC16O9_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6517119.1"
                     /translation="MDYSRYQDLKIRRLEPGILEIVMGDEASGRLSTAGARMHAELAE
                     IWRDVDRDAEVSVAILRGAGKGLSAGGDFGLIEDIIKDFDTRARVWREARDLVYNIIN
                     CSKIVVSAMHGPAVGAGLVAGLLADISIAAKNARIIDGHTRLGVAAGDHAAIVWPLLC
                     GMAKAKYYLLTCEQISGEEAERIGLVSLTCEEAELQAKALEVARKLAAGAQTALRWTK
                     YSLNNWLRMAGPTFDTSTALELLGFTGPEVREGAASHREKRKPVFPPPPKDF"
     gene            15739..16629
                     /locus_tag="AC16O9_10680"
     CDS             15739..16629
                     /locus_tag="AC16O9_10680"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR6517120.1"
                     /translation="MQAFYNLAMRTPDERFADLPGFPWAPHYLQWRGLRVHYVDEGQG
                     GRTFLCLHGEPTWAYLYRRMIPPFVAGGARVVAPDFIGFGRSDKPEDDAFYSFDMHRA
                     FLIEFVERLELRNLTLVVQDWGGLLGLTLPMEMPERFERLLIMNTALGTGDAPLPEGF
                     LAWRAYVAKNPALDCQKLMQRSCPHLTPTEAAAYEAPFPDARAKAGVRRFPQLVPDRP
                     DAPGAELSRKARDWWKSAWQGESFMAIGAKDPVLGPPVMRALRNVIRNCPEPYVHAEG
                     GHFLQEWGEDVAARALASWR"
     gene            16620..17582
                     /locus_tag="AC16O9_10685"
     CDS             16620..17582
                     /locus_tag="AC16O9_10685"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HIR6517121.1"
                     /translation="MALTYADVAAAHERIRAAAKRTPVLTSATIDARTGARVHFKCEN
                     FQRMGAFKFRGAYNALSQLSPEQKKKGVVAFSSGNHAQAVALAGRLLGVPATIVMPTD
                     APKVKLEATRGYGADIVLYDAHNEDRQMIAAKLSAERGAMLIPPFDHPHIAAGQGTAA
                     KELIEDAGPLDLLLVPCGGGGLLSGCAVAAKHLSPACRVIGVEPAAGDDGARSFRSKK
                     LQVIKTPVTIADGARTASLGPNVTFPAILRHVDDFLTVTDAALLEAMFFLWERMKIVV
                     EPTGALAACALLEGKLDARGKKVGVVISGGNVDLAWAAARLQAR"
     gene            17584..19224
                     /locus_tag="AC16O9_10690"
     CDS             17584..19224
                     /locus_tag="AC16O9_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR6517122.1"
                     /translation="MRRLRVLALLASAAMPAAAQDPFPSFEALEAAGAVIGEIRIRAQ
                     DVFDLDDPEEDNWLFRKANALHIQTRPSTLRRQLLFRSGERVSARVIAETERLLRGND
                     YLYRVEIRPTGYDDGIVDLEVVTRDSWTLQPAAKVSLAGGTSSGGVSLEEKNLLGTGA
                     RFAIAARTTSEVSTAGGTRRGVGVDLSYPYAFDGRTTLGYGQSTFEEGSARKARIDRP
                     FYSLEARWAAGAAAARSDQIVDRYANGVVAARYRQSVDSGHALLGVSRGLVRGWAHRF
                     SGGLRYQEETYRLEPGQAAPATLPADRTLVAPFLRYEAVQDNFVELRNLNSIARPEYL
                     ALGWHAWIEIGRAAGGLGSTRSATLYSASLAKGTRIGGEGTLLAAAAVGGEYASGETD
                     RETLGASLRYYQRRGAHTLLFLSLAADATDFSEATQFLSLGGEVGARGFPTNYQRGDR
                     RVTFTAERRFYSDWFPFRLIRVGGAVFFDAGRAWGGPYQKLDEAAAHWATGIGFGLRL
                     LSARSSSGTTVHVDLAFPLKRASGVDAFQVSVQSKTGF"
     gene            complement(19221..21149)
                     /locus_tag="AC16O9_10695"
     CDS             complement(19221..21149)
                     /locus_tag="AC16O9_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HIR6517123.1"
                     /translation="MIQPFPMVRALALLAVLTFASAHAHKPAPLPDVEGRVALGAHVD
                     VLEDPGGGLALDDVRMPEHAARFAPAGAGSLNFGYTRSAWWLRFSLPGGAPPGEELLL
                     EVAYPSIDRLELYVPEPLSGGEARYWARIAGDTLPWDAREVRHRNHVFRFPAPGPAGE
                     HTYYLRAESRGVLTMPLTLWRPAAFAEHKRNAQLVLGLFYGLALALVLYNLMLLFAVR
                     DRVYLFYVLYATAFCTYLLSFDGLGFQYLWPQSVWAANHVLALALSLTLMFGAQFSRT
                     FLDMRRITPRADAVMRGVVAAGVALALFAGTGWVLDHGGVLRAISVIAFAAAAVATYA
                     AVRALILGYRPARYFLLAWAALLAFIALGALRNFTLVPTSFATIYGLHIGFALDVLLL
                     SFALGDRINLLRRERASAEVEALAAQRLLLEATRDSERELEQRIAERTAELNSVNERL
                     RAEASEREALMAQLQAQEQHLRFMAQHDALTGLPNRLSMQQRLALAMELAKRNRKKLA
                     VMLVDLDDFKTINDTRGHPAGDQALVQLAARLRTSVRGSDTVARYGGDEFVLLAGELD
                     RGEDAAMIAEKVADMIQVPLAIEGGAAQVSCSIGISVYPDDAETAEALIALADKAMYA
                     SKAEKDRRYAYYGAGAAG"
     gene            21187..22164
                     /locus_tag="AC16O9_10700"
     CDS             21187..22164
                     /locus_tag="AC16O9_10700"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR6517124.1"
                     /translation="MRAIQIEKAGGPEVLRPTERARPEPKPHEILVKVAAAGVNRPDV
                     LQRQGLYAPPPDASDLPGLEVAGEVAACGAAVTMWKPGDKVCALTHGGGYAEYCVTPE
                     VQALPVPKGLSMVEAASLPETFFTVWSNVYDRGRLAPGETLLVHGGTSGIGVTAIQMA
                     AATGNRVFATAGTDEKCAACLKLGAAQAFNYRTQDFVEGVKQATGGKGVDVILDMAAG
                     DFVPKELKALADEGRLVFIAFLRGPKTELDVNELMRRRLTLTGSTLRPRPVAFKGAIA
                     ARLRERIWPLVEAGKIKPVVFKTFKLEAAAEAHRLMESSAHTGKLVLTV"
     gene            complement(22154..23068)
                     /gene="gcvA"
                     /locus_tag="AC16O9_10705"
     CDS             complement(22154..23068)
                     /gene="gcvA"
                     /locus_tag="AC16O9_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HIR6517125.1"
                     /translation="MSNGNTRLPSLDLLRGFEAAARLLSFTRAGEELHLTQSAVSRQI
                     QELETQLGTPLFERRHRSLALTEAGRQFYATAAQVLATMRAATDRLRAQSGRRALAVT
                     TTHSFAALWLIPRLAGFTRAHPAVDVRISADTRVQDLERDGLDVAIRHGPPSLAGPDA
                     ERLFGERVFPVCNPKLLQDKARPLKAPADLRHHVLLTYDDPDVRHPWLHWRSWLEVEG
                     LSELKPAGRISFSGYEQIIAAAVAGHGVALGRTPLLKDALASGELVAPFKRSADPARA
                     YYAVVSPTAQGRPEVADFIAWLKEEARR"
     gene            23148..23375
                     /locus_tag="AC16O9_10710"
     CDS             23148..23375
                     /locus_tag="AC16O9_10710"
                     /inference="COORDINATES: protein motif:HMM:NF022592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HIR6517126.1"
                     /translation="MRIELDPGALRLARGQTLRVIDAPGSTICVREGSVWITEENMPK
                     DVVLEAGHCYRLSRKGLALVHAFGDAAVALA"
     gene            23518..24393
                     /locus_tag="AC16O9_10715"
     CDS             23518..24393
                     /locus_tag="AC16O9_10715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6517127.1"
                     /translation="MFRDGLLKGKNILVTGGGTGLGKEIAAKYLQLGAGLWIAGRRAG
                     VLEDTAKELTAKHGGTVRTHAVDIRDYAAVDAMIQHIWDASGPLTGLVNNAAGNFISP
                     TKDLTPNGFNAIANIVFHGTFYVTHSIGKRWIAGGHKGSVVSILVTWVWTGSPYVVPS
                     AMSKAGLHIMTKSLAVEWGKHGIRLNAIAPGPFPTEGASKRLRPGSSFGESTKVNPMR
                     RVGEMSELQNLAAFLMADGCEWLSGETIAIDGAGYLATGAYFTELDKMTDADWQKARE
                     LIKAQNDKDRAGRSA"
     gene            24402..26069
                     /locus_tag="AC16O9_10720"
     CDS             24402..26069
                     /locus_tag="AC16O9_10720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6517128.1"
                     /translation="MTDKLVPRPAATLLLLRDGSLGPEAFLLQRTHDAAFLGGAHVFP
                     GGALDKADRDPRVLRRVAGLTDAEASARLGVDAGGLAFWVAAMRECFEEAGILLAEGE
                     DGRPLTAARAVALAPSRALLHSGKLGFHEFLEKERLLLRGRELAYFGHWVTAPGRARR
                     FDTRFFLALAPETQRGAHDGTELVASAWLRPQEAIEREARGEIQLVFATKNTLADLVR
                     FARAKDALEHAREAEVETNRACWALAPDGAAALFRRRDPQYFEIHWSDPEETGQTSVA
                     LAPGLAKRLDPLVTRVIAPNPGMMTGPGTNTYLVGESELAVIDPGPAIDEHIAAVLAA
                     GAGRIRWILCTHTHMDHSPAAAALKAATGAVVIGRPAPDHPGQDRDFAPERVLAHGDR
                     LMFGGFTLRALHTPGHASNHLCYLLENTRMLFTGDHVMQGSTVVINPPDGDMRAYLAS
                     LELLLGEDILILAPGHGYLIGEPHREARRLIRHRLAREQKVVWALQRLAAPTAEALVS
                     EVYDDVAPRLHGVALRSLSAHLDKLALEGRARAADGRWSLVQSSTVE"
     gene            26089..26355
                     /locus_tag="AC16O9_10725"
     CDS             26089..26355
                     /locus_tag="AC16O9_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517129.1"
                     /translation="MGSYEQTLKLVTNLDRAAIEARLADVRGTAQSAGLAEVAQQLVH
                     APGAPRGDLEQRIHGALKLLAGKPEHKALQAQLELVEINLPNLK"
     gene            26371..27711
                     /gene="gor"
                     /locus_tag="AC16O9_10730"
     CDS             26371..27711
                     /gene="gor"
                     /locus_tag="AC16O9_10730"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR6517130.1"
                     /translation="MKFDFDLFTIGGGSGGVRASRVAASFGARVAVAESGRFGGTCVN
                     VGCIPKKLLSYAAHYREDFAVAKSFGWSVGEPTFDWRTLVANKDREIARLNGVYENVL
                     TKAGAKILRARATVIDPHTVEADGRRHTARHILVATGSRPQVPQIPGRELAITSNEAF
                     FLERLPRRALVVGAGYIALEFASIFNGLGVHTTLAYRGARLLRGFDADLGERIAEEMR
                     KKGIAVRLHTNPAKLESGIRVTYEDGATQEVDLVLFATGRKPNTAGLGLEGAGVQLGA
                     GGAVAVDEFSRSSVASIHAIGDVTDRLNLTPVATAEGMALAKTLFRGEPTPVDHVNVP
                     TAVFSDPNLATVGLSEEAARRHCSEVDVYKTAFRALKLTLGGHEERTFMKLVVDAASQ
                     RVVGAHMIGPEAGEIIQGVAIAVKLGATKAQFDATIGIHPTAAEEFVTMREKAS"
     gene            27714..28928
                     /locus_tag="AC16O9_10735"
     CDS             27714..28928
                     /locus_tag="AC16O9_10735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6517131.1"
                     /translation="MVLESQIEKMVGQLRASAGLPLAIELWNGKRYALGERPTVTLRV
                     PAASAVKYLAKADLAALGEAFVEGHIEVDGPIGDAIRAAESLARRLGGARKGRVPGLL
                     RNMHSKKTDSAAIRYHYDVSNDFYALWLDRRMVYSCAYFRTGEESIEQAQEHKLDHIC
                     RKLRLAPGERFLDIGCGWGALVLHAAERYGVRATGITLSENQHALANERIRAAGLQDR
                     CEVRLQDYRDVPGEGAFDKIASVGMFEHVGLKNLPLYFGVIRRLLADGGIALNHGITS
                     IDPDSRSVGLGAGDFIGKYVFPHGELPHLSLVIREMGAAGLEVMDSETLRLHYAKTLQ
                     HWSGRLEANLDQARKFAGEKRLRIWRAYLAGCAHAFGEGWVSIQQVLAVKTGSDPRRN
                     PLPWTRDYMYQR"
     gene            complement(28925..29788)
                     /locus_tag="AC16O9_10740"
     CDS             complement(28925..29788)
                     /locus_tag="AC16O9_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6517132.1"
                     /translation="MPAGGRVPRRRAPQGDPAARARQRQDHGPLHAQPGGEGLSELLG
                     GQWELLALIPIAFAGALVFGLTGFGSALVTMPLATHFVPLQLALALYALCDLSSALRV
                     GFESPRNAVKAEWMRLVPMILAGTALGVTVLVNLPRAAGMAALGAFVFGYALYSLFRH
                     ESHRVIAAGWAWIAGLAGGVTSTLFGAGGPPYAIYLSQRGLTKEQFRATMGFATLTSI
                     SLRVAAFLLTGLLLEPKVWLVAIGAVPAAMLGIAVARRMYLRISREQLLRAVAAVLLL
                     TGGSLVLRALG"
     gene            complement(29671..30300)
                     /locus_tag="AC16O9_10745"
     CDS             complement(29671..30300)
                     /locus_tag="AC16O9_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR6517133.1"
                     /translation="MILSSDSFSDKGAIPGKCAFGVADAKTHVKLSDNRNPHFAWRDL
                     PAGTKSLVLVCGDVDVPSKPDDVNKEDRQIPATLPRIEFFHWLLVDLKPDAGPIREGE
                     FSSGVTAGGKSGPAGPRGTRQGVNDYTSWFASDKDMAGEYFGYDGPCPPWNDSIVHRY
                     VFTLYALGVDTCPLEGAFRGAELLKAIQPHVLGSAKITGLYTLNPAVKA"
     gene            complement(30297..31064)
                     /locus_tag="AC16O9_10750"
     CDS             complement(30297..31064)
                     /locus_tag="AC16O9_10750"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR6517134.1"
                     /translation="MSRVPALTVSEARGVRTLHVGGEAIQSSMRLSDPFALELEYTRC
                     MMTFLLFHPRPARALKIGLGGGSLVKFFWRRLPRLRTRVVELDPRVVQVARAQFHLPA
                     DDARLVVEIGDGAEALAPECCDLLVVDAFADEHPPAALVSQAFFDAAWLALESPGALV
                     LNLMNDDPKLDERIARVERAFGGAVLALPALYDPNVIVIALKGAPRALSWDTLASRAH
                     GLERRFGLPFPRYVPRLKRMNRFDAGALIIVPEGGGA"
     gene            complement(31061..32242)
                     /locus_tag="AC16O9_10755"
     CDS             complement(31061..32242)
                     /locus_tag="AC16O9_10755"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517135.1"
                     /translation="MPRPGWGVVLAAFTLMFMGFGAAYSFAAFFAAFEAEFGASRAHV
                     SLVFSLSALLWFSAGVPGGMLADRFGPRGVCLAGALCLAAGLALAANAPSVELLYLTY
                     SLGAGLGIGLSYVPSVAAVQQAFVQHRALASGIAVSGIGAGNLVMPLAAAWLIGLVGW
                     RHAYLALAAAMLLLSVPAALALGGRKSVRRGAGGAPLPGMTLAEGLRSPVFWMLYGVA
                     LLICVGFFVPMVHLVPYAIDVGLSEAQGVSLVSLLGLGSLLGRFFVGGIADRLGHLRA
                     LMGVGAGLGLMFVLWWASSSYLALAVFAVVFGTLYGGYVALAPTVCMDIFGPRALSAI
                     IGALYTAAGLGTLVGPTFAGASFDATGSYDLSILACAALSFAGATLAVLMRRHLPQRH
                     A"
     gene            complement(32250..32675)
                     /locus_tag="AC16O9_10760"
     CDS             complement(32250..32675)
                     /locus_tag="AC16O9_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6517136.1"
                     /translation="MKRRNLNPKALFDPRFFTHTVALERAATLVFVSGQVSYDRDGIV
                     VGKGDMRAQAEQVFASLTHNLKAAGATWRDVVKMNGYMVGLSAEAVNQYREVRARYLD
                     PAAMPASTLVGVERLVHDDLLLEVEVIAALPAARTRAQR"
     gene            complement(32672..33784)
                     /locus_tag="AC16O9_10765"
     CDS             complement(32672..33784)
                     /locus_tag="AC16O9_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HIR6517137.1"
                     /translation="MRPPAAIGQRLEEVDTPALILELDAFEANLRTLADAVRGTGVRV
                     RTHAKTHKCPDVGKRQMALGAVGACCQKVSEAEAMVAGGITDVLVTNEIVGAQKLERL
                     ARLAGRARIGVCVDDADNARAADAAAARAGAKLDVYIELEVGMGRCGVAPGEPALALA
                     RVVAGCSNLRFQGLQAYNGRAQHVRSIAERRALIERAGEHVRATRALLEKNGIECPTV
                     TGAGSGTFMLEVASGAWNEIQPGSYAFMDADYARNEQSAPLPRFAHALFVLTTVMSRG
                     GGARAIVDAGLKASSVDSGMPAVWQRPGLSYVQASDEHGFIEVAPGTAAPAPGERLLL
                     VPGHCDPTINLYDWYVCVRKGVVESLWPITARGALT"
     gene            complement(33781..>34055)
                     /locus_tag="AC16O9_10770"
     CDS             complement(33781..>34055)
                     /locus_tag="AC16O9_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517138.1"
                     /translation="VLLGVGWNFLFIGGTALLTETYRPEEMAKAQGANDQAIFIMMAI
                     SSFTSGLTVTAAGWERVNLFALPMIAVVAIAIVWYGMTHHRRTGVA"
BASE COUNT         5201 a        12156 c        11686 g         5012 t
ORIGIN      
        1 cctaacccag ccccggaaac agcgccttca gccccaccgc catggtctgc accgccatgg
       61 cggcgagcag cagcccgagg atccgcgtcg ccacgttcat cccggtggtc cccatgcgcg
      121 cggccacctg gtgcgccgcg cgcagtgcga gccacaccac gccgcacacg acggcgatgc
      181 acgcgatgat gatcacctgg tgcgcggcgc ccccgccttc tgtggcgatg atggcggtgc
      241 tgatcgcgcc cggccccgca aggagcggga tggcgagcgg caccacgccc tgcagctcgc
      301 cctcctcgag ctcctcggcc tcggccttcg actggcgtac gccgcctgcc tgcgcgttga
      361 gcatcgccag cgccatgagc agcagcacca gcccgccgcc gacccgaaac gccggcaggc
      421 tcgcgccgat caggtcgagc agcgcctcgc cgacgagcgc cgccaccgcg agcaccagga
      481 acaccgtgac cgccacggtg cgcgcgaggt gcttgcgcgc gtcttcgccg tgacttgcgg
      541 cgaatgtgag gaagaacgga acggcgagaa acgggtcgac gaccgcggtg agcgtgacga
      601 cgaagcgcag gtaatcgccg agcggcgcga gcccggccac gcggctactt ccttgccgcg
      661 gcgctcgccg ccttgcgatc ctcgttgact ttggcaaacg ccgggcgctc gcccagcatc
      721 ttcaggtagg gcttcacctg cggcatgtcc tcgaggaagt cgcggccgag gaagttgcgc
      781 gtcaccagcg tcaccagcgg caggtgcacg aacgccgcgc agtcggcgat ggtcagctca
      841 ctgcccgcaa tgtaaggctc gaacttcgcc actgccttca gcgcgcgcac gcccttggca
      901 agatcctttt ccacgcgctg ccgtgtttcc tcggacacgg gcttgccgcc cgcgagcatc
      961 ccgtacatgc gccgcgccac gagctccagg tgcagctcga tgaagatgac gagctcgcgc
     1021 acgcgcgcgc gctcgagcgg gtccttgggc agcaggggct tttgcggaaa ggcgtcctcg
     1081 aggtactcca ggatcgcgct cgactcgcgg atgcgcgcac cgttcacttc cagataaggc
     1141 accttgccca tcgggctcct gaccaggtag tcgccttcct gcgagggctt cacgttctcg
     1201 tcctcctcga acgcgacccc tttctccagc aaggcgatgc gcaccttgtt gtggtagttg
     1261 ctgatgcgga atccgcacaa cctgatcatc gcttctctcc ctcgtgcttg gcgtagatct
     1321 tctgcatgcc gccgatccag cgatcgtagt cggcggtctt gttgcgcatg tagttcagca
     1381 ccgtggggtg cggcaggatg agaaatcgct cctctgccat cgccttcacg atatcgcccg
     1441 ccacctgctc ggcgctgagc gcgccgtcca cgcctgccga gccgccctcg cccttgtaca
     1501 tgccggtgag caccgcctgc gggcacaggc acgatacgcg cagcccgcgc cggccgtagg
     1561 cgatcgcgag ccactcggcg aaggccaccg cgccgtgttt ggtgacgcca taagtcgccg
     1621 attcgacgat gttcagcagc cccgcggccg acgccgtgac gaggaaatag ccgccgccgc
     1681 gcgccaccat cttctccacg acggcacgcg cggcccacac gtgcgccatg acgtggatgc
     1741 gccagttgcg ctcccagtcg gcgtcgctcg cctcgagccc gccgcggcgg atgatgccgg
     1801 cattggagaa gtagacatcg acgcgcccgt ggcgctcctc ggtctcccgc accaggcgct
     1861 ggatgtcggc ttcgcggccg acgtcgcagg ccaccgccag gcccttggcc tcctcagcca
     1921 ccggattcaa ggccgccatg ttgagatcgg cgagcaccag gccggccgcg ccctcctggg
     1981 cgaagcgcaa tgccgcggcg cggccgatgc cgctcgcggc gccggtgatg acggcgacct
     2041 tccccttgag atccatcgtc agccgcgctt gcggccggcg agcgcctcgg tcaccgaggt
     2101 ctgctcggcg atctcgagct tggcgatggc gttgcggtgc acttcgtccg ggccgtcggc
     2161 gaaccgcagc gtcctctgat gcgcgtacat gttggcgagc ccgaagtcgt ccgagacgcc
     2221 gccgccgccg tgcacctgca tggcccagtc gatcacctgc ccggccatgt tcggcgccag
     2281 caccttgatc atggcgatct ccttgcgcgc caccttgttg ccgaccgtgt ccatgcgcca
     2341 ggccgcgtgc agcacgagaa agcgcgccga atcgatcatg atgcgcgcct cggcgatgcg
     2401 ctcgcgcgtc acgccctgct cggccacggt gcgcccgaaa gcgacgcgct gcgtggcgcg
     2461 cttgcacatt ttctccaacg cgcgctccgc cgcgccgatc gagcgcatgc agtggtggat
     2521 gcgcccgggc ccgaggcgcc cttgcgcgat ctcgaagccg cggccctcgc cgagcaggat
     2581 gttcgacgcc ggcacgcgca cgtccttgaa ctcgatttcc atgtggccgt gcggcgcgtc
     2641 gtcgtagccg aacacgttca ggttccgcag gatcttgatg ccgggcgtag ctgccggaac
     2701 caggatcatc gattgctggc tgtggcgcga ggcgtggtcg gggttggtct tgcccatcac
     2761 gatgtagatc ttgcagcgcg ggtcgcccgc gccgctcgac caccacttgc ggccgttgat
     2821 cacgtagtcg ttgccgtcgc gcttgatgtg catctggatg ttggtggcgt ccgagctcgc
     2881 caccgccggc tcggtcatca ggaacgccga gcggatctcg cccgcgagca gcggctcgag
     2941 ccactgcttc ttctgctcct cggtgccgta gcgctcgatc gtctccatgt tgccggtatc
     3001 gggcgccgag cagttgaaca cttccgaggc ccagctcacg cgccccatga tctcgcagag
     3061 cggcgcgtac tccaggttcg acaacccctc cggcgcgcgc ttcgaatgcg gcaggaagag
     3121 attccacagc ccggcctttc gcgccttggg tttcagctcc tcgatgatcg gcaccggggt
     3181 ccagcgcttg tcgccgcgcg tatgcgcgta gtacgcgctc tcgttcggat agatatgctc
     3241 gtccatgaaa gcgatgagcc gctttctcag ctcctgcacc ttcggcgagt actcgaaatc
     3301 catgacgttc tcctaggcag ctctcttaag tatcttctcg acctgcgccc accccagctc
     3361 ggcgagcggc cgcgtgcgcg agcccgcctc gcgcgcgtac tcgctcgccg ccgtgccgtc
     3421 caccacgcgc ttggcgatgc cctgcgtgat ggcggcgatg cggaacaggt tgtaggccat
     3481 gtaatagtcc caggtcgaag ggtcgatgcc cttgcgcccg gtgcgcctgc aatagcgctc
     3541 gacgtactgg cgctcggtgg gaatgccgag cgcctcgagg tccagcccgg cgatgccgcg
     3601 gaacttgccc ggcgcaatgt gccagctcat gcagtgatac gcgaaatcgg cgagcgggtg
     3661 ccccagcgtc gagagctccc agtcgagcac cgcgaggatc ctgggctcgc tcgggtgaaa
     3721 gatggcgttg tccagccggt agtcgccgtg caccaccgag gtctcgtccc cggcgggaat
     3781 gttggccggc agccactcga tcagccggtc catctcgtcg atgcgctcga gttcggaggc
     3841 acggtactgc ttgctccagc gcgacacctg gcgcgcgatg tagtcacccg ccttgccgaa
     3901 atcctccagt cctaccgcct tgtagtcgac gccgtgcagg agtgcgatga cacggttcag
     3961 ctcgtcccag atcgcgccgc gcgcttcgcg cgtcatgccg ggcagggcct ggtcccacag
     4021 cacgcgcccc tcaacgcagt ccatgacgta gaacgcggta ccgatgacgg actcgtcctc
     4081 gcacagcacg tagggtttcg caaccgggaa accgactccg tgcagggccc ggatgacttt
     4141 gtactcgcgc tccacggcgt gcgccgaggg cagaagcttt ccggggggct tgcggcgcag
     4201 cgcatagcgc ttgtcgccgg ccgtgatgcg gtaggtggga ttggactggc cgcccttgaa
     4261 ctgctcgatg gcgagctcgc cgcgccagtc gtcgatcttg ccgcgcagga actgggccaa
     4321 ccggcctgcg tcgaagcggt ggcgttcctc gaccggcttg acgccgatga actgctcgaa
     4381 catgccgcgg agcttacctt agaagccccg cgccgagtat cgccgcggcc gcgctagcag
     4441 ccgttgtcgt gccgggccgc gccgttttcg ggctgggcct tgtcggcacc gcgcgagccg
     4501 atgaggctgc gcagcatcgt ggtgatcagc tcgcggtcca cgggcttggc gatgaacgcc
     4561 gttgcgccga gcaggcggca gcgctcgcgc gattcgtgag tcgacatcac cgacaccacg
     4621 aggatcggcg tcgcctggtt ggcaccgcgc tcgcgcagct ggcgcagcac ctcgtagccg
     4681 ccgatgccgg gcatcagcag gtcgaggaga atgacgtcgt agggcgcttc ctcggccagg
     4741 cgcagcgcct gctcgcaggt cccggcgagc gtgacgcgcg caccgagctc ctgcgtgacg
     4801 atgcggccga tcaacagccg cgtcggctcg tggtcgtcga cgatcaggac gttgcggcgc
     4861 tcggtggcca tgtcgggccc agtgtgaggc gacttagcgc gtcaggtacg ctgcgccgga
     4921 caggctgcgg tcggccggcc cgctgaggcc gaacgttgtg atcaggttct cggcctgcgt
     4981 tccgacaaac gtgccgtgcg cgatgccggc ctggccccct ccgcccttga ggcgcacgcc
     5041 gcccacgccg gtgaagtcgg cgatggcgcg gggagccgtc gtatcgaggt tcacattcca
     5101 gttgtagcca ctggcggcgg tcgcattgag gtcgtagcgg gtgactacct ggctgctgaa
     5161 gtcgacgccc acgctcatgg agttgagcgt tgcctgattc ccgagctgat ctgtgaacgc
     5221 aggtccgcca gtgtagttat acgtggcact catcacggcc ggcgacagcg tagccaggtc
     5281 cgtcctattc gccgtggaga tcatgtgcgt cgagccggcg gtgggctggc cgccgacgac
     5341 cgcgacgccg ccctgccacg tggtccagtt gatcgtgccg gcgggggcgc tcccgccggt
     5401 ggaaggcgcg ccgttgtcga gcacggtgac gccgttgccc gagttgccga accggcctcc
     5461 gccgaccgca gtcacgaaac cgaccagcgg gccgttgccg gtgcccggcg tcaccagcgg
     5521 gccgcgcgtg cgcagcagcc gcacgccatt ggtcgaccgc gcgacttgct gcacgacctc
     5581 gtcgacttcc gcagcctcgg gcgcggggtc cgcggcaggc cgtgcgttgc gcggcgcgat
     5641 cgagcggttg aagaagcccg gcaccatcgg caggatcacc ggcacgtcgt tcgcgctcgc
     5701 cacgtacccg acctggttcg gctcgatctc gacttcgccg gcgcccgtgc ggatgaaggt
     5761 cttgccggta ttgaccttgt tgtaggcgcc gggcttggcg tcgtcggcgc tcaccggtcc
     5821 ggtcgccgga aagtaatagg tttcgtggtc ggtcccgcgc acgcccatgt gggcaatcgg
     5881 cgtctcaatg acgtagttcc tcttgtaggt gcggccgata gcaccggtca cggcgcgaaa
     5941 gccgccgtgc tccaggcggt agcggacttt ttcggtgccg tcttcgaccc cggcatagcg
     6001 gaactcggcg accgtgacgc gcgaatcggg caccacgaca acaatggcgc cgtcgcccat
     6061 cttcagctgg gccatcgcgc cttttgcggt cgccagcgta tcgccgactt ttacgggcac
     6121 gcccttgctc gcgaggcgcc cgacgccgtc gggctgcacc agctgcacgt tgccggcgac
     6181 gaactggatc acgccggcgc tctgcccgag cgccgggctg acaaaggcgg cgccgagcgc
     6241 gaaacttacg aagacgagac tatttctcat taatccccca acgccctgcg ggaagatgtg
     6301 gatgttgccg atagtgtgca actcccaggc ctgtgtacag gtaaaggatg ccgcaacctg
     6361 acaattcctg accggggcct aattgaacac gcgccgcagg gtcagcgtcc agtcggtacg
     6421 gcggaactcg tagatctcga tattcgactc ctgcaccgac cgcaggacgc cgaagcgcaa
     6481 ctccagtttc gacgagagat cgtagcgcaa gaagccttcc aggtccgtcc gccggtcgtc
     6541 acggcgcacg agaaaggcgg aatcgaagcc gtcatagcgc gactcttgca cgctggcgag
     6601 cgcgctgaac cgaaggtccg gaccaaggac gtgctcccag gcgaagcgcg cccccacccc
     6661 gcggcggtcg ccgcccgggt tgccgccgcg atcgttgtcg ccgcctgcca tgaatacgac
     6721 actggctgtc gatttccggt caatcggcac ggtggcgccg acgccggcag cgacgaagtc
     6781 agcgtcgaag atctgcaggt ccactggggg atggcgaaag ccgccggcgt ggaggtaggc
     6841 gaacatggcg ccgggcgtcc ccggcgccgg ggacctttcg gcaaagtcga ggctgaaggc
     6901 gcgcacctcg cggctggtcg agccgccgag cgtgttgcgc accatctgca actggctgcg
     6961 gaccacgtgc cgctctcccg cccaggcaag cccggtgctc gcggcgacgc cgcctacatc
     7021 gaacgccgtt tcatgcgcgt tccaccggct gaggaactcg gcgttgccga tgagcgacac
     7081 gcggccacgc aggggcatgg cggcctctac gccgccgccc acacccgtga agccgtcgtc
     7141 cttgcccacg ttctggtcgg caagctgcag caccgccacc agtcccggca cgaagacctg
     7201 gctctggccc ggcgcctggt tcacgttcgt gctggtgccg gcgaacacgc tcaggtagcc
     7261 gcgcgcggcg gcacgggccc ggctctcgcc gcgcgactcg cccaggtaca gcagcagttg
     7321 cgcgcgcagc gcgggcggcg ggtcgagcgc cagcagggcc tcgaagacga cgcgcgcctg
     7381 cgcccagttt tccagcgcaa gatacgcacg gcccatgtcg atgcgcgcgc cggcgtggcc
     7441 ggaatcaatc gccagcacgc gcgaaaaggc gatggtcgcg cggtcgggcc gacccgcgtt
     7501 cagtgccgcg cgcccgagcg cgtaatcgaa atcgacctcg ccgatgtgcg tgtcctcttg
     7561 cgccgcaagg agcgcgtagg cctcggcgta ccgcccggcc tcggaaagcg cactcgcgcg
     7621 cgccagcagg tccccgatct cggattgcgc ggcgacgcga cccggcccag ccatgacggc
     7681 agcggcgaca aggacggcaa gcagcaatcg aagcaatcgg cgcatgtttg tcttcttgac
     7741 ctcggcgcat ccgggtggct cgtgccgcac acgtcccgtg cggacggccg cgctgaggaa
     7801 ttgctctaga attgtcgctc acaatgtccc ggcaggacag cgtgcaatca gatgcaaccc
     7861 tcccctggct gcgcgtgctg gtctcggctg ccggggggct gctgttggca gcccttttct
     7921 cccagagcac gctgttttcg cggctgtcgt ggtgggtcga tgacgcactg cagcgccgtt
     7981 tcgccgtcgc gctgccgctg gacggcgttg ccgtggtcga cgtggacgag gcttcgctgc
     8041 agaggctgca gccgcggctc ggcgcctggc cgtatccacg cgacgcgtat gcccaggcgc
     8101 accgcttcct ggtcgcgaac ggcgcgcgcg tcgtggctta cgacatcctg tttgccgagc
     8161 cgcgcgccgg cgacgcggcc ttcgccgagg cgctcgatcg gcgcgccgtg ctggcggccg
     8221 cggccttgcc ctatccctat gcccggtcgt ctcagtacca ggagcatctc gcgcgcattg
     8281 cgctgtccga cgtacaggac gatgcgccag ccgcgcagct cgcgcacgcc tggccggacc
     8341 tgacgctgcc gctcgagcag ttcactcagc ccggcaaggc gcgagtaggg gtcatcagca
     8401 cgctggccga cgacgacggt gtggtgcgcc gtttgaacgt gctgcacctc gcgcacggca
     8461 aggtgctgcc cggcttcgcc gtggcggcga tgctccccgg cggacccgag cagccgctgc
     8521 gcgtggagcg cggccagctg cggctggggg atcgcacttg gccgctggcc gcggacggga
     8581 gcgttgcacc gcgcctgccg gccaacctcg ctgacctcgc cgtgatcccg ttcttccagg
     8641 tgctggatgc cgcggagggc gtgtcgggca cggcgcacgt ggccgacctg gtgcgcggcc
     8701 ggcacgtgtt tgtcggcagc tcgagcacgg tgctgggcga cgtggcgctc acgcccgcgg
     8761 gtcgcttccc cggactgcac gtgaacgcgc tcgtcgccgc cagcctgcaa gccggcgaag
     8821 tgctgcgccc gccccgagcg tggctcgaca tcgcgctcgc cgtgctggcg cttgcgctgc
     8881 cggccgcact ggcgcgcagc ggcgttgcgc tgcggccgcg cgacttcgcg atcgcactgg
     8941 gcctgggcgc ggttctgctc gccctggcgg tgattgcgct ggccgcggcg cgccagcaat
     9001 cgcactggct gtttgcggca acggccggcg tcggtgcgct cggcgtcgcg gttgcggcgt
     9061 ggctgtttgc cctctaccag gaaaagcagc gcctgttcta cgagaagctc gcggcgctgg
     9121 aggccagccg cctcaagtcc gagttcctca atcacatgac gcacgagctg cgcacgccga
     9181 tcacggcgat catgggcttc aacaagttca atctgtacgg cgacgacatc gggcgtgacc
     9241 agcgcctgcg ccacagcgcc atcatcgcgc gcaactgcga gcacctgctg gcgctggtca
     9301 acaacaacct cgacctggcg cgcatggagg cgggccagct gaagttcgag cgcacggcgc
     9361 acgaagtgcg cgcgctgctc gacgacgtcg tcgcaacgct gcgcgtcatg gcgcacgaca
     9421 agggcctagc gctcgaacag gaggttgccg ccgacatgcc ggcggcgctg tcgctcgacg
     9481 cctttcgcgt gcgccaggtg ctgatcaacc tgatcggcaa cgcgatcaag ttcaccgcgc
     9541 agggcaaagt cgtcctcaag gcgtcctggc actcgggcga gttgcgcttc gagatcgccg
     9601 ataccgggcc cggaatcccc caggagagtc tggagcggat cttcgtgccg ttccagcgtg
     9661 cccccggggt caccgcggcg ggcaccggcc tcgggctctc gatcacgcgc aagctcgtcg
     9721 agctgctggg cggatcgatc cacgcaaggt cggaggtggg caagggctcg gtattcgagg
     9781 cgcgcatccc ggcgccgccg gcgcagctgc cggcgagcac ggtgtcgagt gcgccgacgc
     9841 cgatgccggc gaagctggcc ggccgcatcc tgctggccga cgacaacgcc gacctgcgcg
     9901 cgctcgttca ggtgcagctc tccgatctcg gcttcgagtg caaggcggtc ggcgacggcc
     9961 tggaggcgat cgaggcggcg ctggcgcagc cctatagcgt cgtgctgctc gacatggaca
    10021 tgcccttcat ggacggctac gagacggtgc gcgtgctgcg cgagcgcggc tataccgccc
    10081 ccgtgatcgg cttcacggcg caccaggcgg gcgcgccggt ggagcgcgcg ctgatcgagg
    10141 gctgcgacga tgtcatcagc aagccggctt cgcccgagcg gctgcgcgag gcgctggcgc
    10201 cgtttgccga aggcgcgctg cccccgcagc ccgccgccgg cgccgacgcc atccgggtgg
    10261 gcgtggacgc ccgcctgcgc ggcatcgtcg ccaagttctt gagcaactgc gggcgcgatg
    10321 tccagcggct gcagcacgcg ctcgacggcg gcgacttggc caccacgcgc gccatcgggc
    10381 acgcgcttcg cggcgcaggc ggcagctacg gcttcgacga gatcacgcgc atcggcaagt
    10441 cgatcgaaga aagatccatg cacggcgacg ccgcgagcct gggcccgctc gtcgcgcagt
    10501 tgcagaatta cctttcgcgc gtgcaccccg agttccgctg agcttggctc aggcctgcga
    10561 cgcgggcgca gcccgcagcc agaacgtcac cggtccgtcg ttcacgaggc tgacttgcat
    10621 gtgggcgccg aagcgcccgc tcgccacctc ggcgtggcgc tcgcggcagg cgcgcacgaa
    10681 cacggcgtag agccgctcgc cgtccgctgg cgccgccgcc gtcgagaatc cgggccgcgt
    10741 gccgctcgat gtgtccgccg cgagcgtgaa ctgcggcacc acgagcagag cgccggcgat
    10801 gtcggcgagc gagcggttca tccggccctc ggcgtctgcg aagatgcggt agccgagcac
    10861 ccgctcggcg aggcgcgcag cctcgcgctc ggtatcgccg cgctcgacgc cgacgagcgc
    10921 cagcacgccg cggccgatgg cgccgagctg ctctgcgccg gcggcgacgc gggcctcggc
    10981 gacgcgttgc accaatgcga tcaagcgatg atgtcgggaa tcagctggtc gcgcaggcgc
    11041 gcgagcaggt ccttcagcaa tagcttcttc ttcttcagcc gccgcagctg cagctggtcc
    11101 tgcgccgggt cgagcgccag ccgggcgatg atgtcatcca ggtcgtggtg ttcggtttcc
    11161 agcgccagga tgcgcgcctc gatctgcgcg cgctccagcg ccgtgagcgg ctgattgctc
    11221 aaggcgcgcg ccctgcccgg cgccgcgtac gcacaaataa ttgcagaagc ccccggcagg
    11281 tcgtgaagga gttcaccgct tccatgggcg tgtgttgagc atcctagcgc ctccattcgt
    11341 gagcgcctag ggggtccgga ccgcaatgcg ccgcaacgat cacgacgtct ctgcccgcat
    11401 ttgcaccact gacgccgacg ccgtgcgcac cgcgatcctg cagctgtacc aggggttgta
    11461 cccgggcacc gccgacagca cgattcgcgg cgctttcgac tacctggtgc atctctaccg
    11521 cggcggccac cccgagttcc acgcctgcga cacccagttc cacgataccc agcacgtgct
    11581 cgaggtgaca ctcgccatgg cgcgcctgat ggaaggctac gagcgctccc gctcggaggg
    11641 cgccccgctg tcgccgacgc tctacagcgt gggcgtggtg agcgccctgt tccacgacgt
    11701 cggctacctg cgccggcgca gcgaccgccg gcacgcgcac ggcgccgagt acacgctcac
    11761 gcacgtgggc cgcagcgcgc gcttcgtgag cgcttacctg cgcagcgtgg gcctcgcgcg
    11821 ctacgcgggc gccgccgcgg cgatcgtgca ctacaccggc cacgagcgtc ctgcggaggc
    11881 gatccgcatc gacggcgccc tgccgcggcg catcggccag atgctcggca ccgccgacct
    11941 gatcgcgcag atggccgacc gctgcttcct ggaaaagcgc cgcgaccgct tgtacccgga
    12001 gctcgtcgca ggtggcgtgg ccgggcgttt gatccgctct gcgggcgagc tggtgcggcg
    12061 cacgtatgag ttccatctgc gcgcgatgcg ccggctcgat ctgcagctgg cgcgcgccta
    12121 cgggtacgcc gagcgccact tcggcggcca gaatctgtac ctcgaggccc tgcagcagaa
    12181 cgcgcgctat gcgctggtgc tggcgaacgc cgacgcggcg gggcgcctgc gccgcgcgcc
    12241 gcccgctcaa tagctcggct tgcgccgaaa gtactgcagc cggtcaatcg agcgcacgcg
    12301 cagctccttc agctccgagc cttgcagcac gcgtaagggc acctcgacgc cggccgcgcc
    12361 ccgcgcccac agcttgcgat agaaatccgc aagcgaggcc acgtcctcgc tgccgacgcc
    12421 gatgacgagg tcgccgcccg ccaagccggc ccgatcggcc ggaccctcgc gcgcgacgcg
    12481 cgtcacgaac agccgcccgc gcacctcctc ggcgtagacg ccgagccagg gacgcctcgg
    12541 ccccggcgcg cccccgtgcg cgatcaggtc gtcgaggatg ggctgcagaa ggtcgattgg
    12601 cacgaacaga ttgccgggcg cgggcgtgcc agccccgccc gcatccggca cgatgagcga
    12661 gccgatgccg agcagctcgc cgcgcgcgct gattagcgcc gcgcccgacc agttgtgcac
    12721 cggcggataa gtgaagatgg cggcctcgag caggtactcc cagctgcccg agaacggccg
    12781 ccgcgacact acggtgacga ggttgagcgc ctcgcgcccc ccgtagccca ccaccatcgc
    12841 cgcatcgcgc tcggccagcg ccgcggaatc accgagcggc agcggcttgc ccgcgagcgg
    12901 cgccgcgagc ttcagcagcc cgaagccggt ggcgtggtcg taggccgcga gcgtcgccgg
    12961 cgccgacttg ccgtcgccgc cggtcacctc gatcgcatcg gcttcgatca ccaggtagcc
    13021 gatggtgagc acgtagccct cgcgcaccag gacaccggag ccttcgcgtc gcgcgccgag
    13081 cgtcgccagg gtgcgggcgt cgggcaggat gcgcgactgc acgcgcacca ccgccgacag
    13141 cgtcgcctcg gcatcggcgt cgggcctgtc ctgcgacagc gccggccccg cggtgcacgc
    13201 gagcgcgagc agaaggctgc gacagatggt tggcatcggg cacctccggc gcgcggatgc
    13261 gcgcgcgaat attgtagtct cgcgcgcatg cgcctggtga gctggaacct gcagtggtgc
    13321 cgcggcatgg acggcgtcgt cgacccgcgg cgcgtcgcgc gcaccgtgcg cgagctggcc
    13381 gatgcagacc tgtgctgctt ccaggaggtg gcacaggggt tcggggcgct gccgggaagc
    13441 gccggcgagg accaggtcgc ggcgctcgcg gccgagtttg cgggttacag cgcgcactat
    13501 gcgtgggggg tggacgtcgc cggcgccgcg ggccggcgac ggcgtttcgg caacctgatc
    13561 ctgtcgcgcc tgccggtcgg gcgggtgctg cgccacggcc tgccctggcc gcccgatgcg
    13621 ggcgtgccga gcatgccgcg cgtcgcgatc gaagccgtgg tcgcggcgcc gtggggtcag
    13681 gtgcgcgtgg tgacgacgca cctcgagtat tactcggcgc ggcagcgtgc ggcccaggtc
    13741 gagcggctgc tcgagctgaa cgcggaggcc gccgcgcacg ccgccgcgaa acctgccgcg
    13801 cgcgacgacc aaggtccgtt tcacccgcaa ccgcggcccg cggccggcat tctcacgggc
    13861 gacttcaaca tgacccccga ggacccgctg gtggcacgcc tgggaagcgc ttacgccgac
    13921 gcctggcgcc aggcgcaccc cggacggccg cacgcaccca cgttccgcct ggagagcgcc
    13981 gacgaggcgc cttattgttg cgacttcgtg tttgtcactc cggatctcgc ctcgcgcgta
    14041 cgttcggtac gcgtggatgc gcagacgcgc gcctccgatc accagccggt gatcgtcgag
    14101 ctcaactgat cttcatctca cgaggcgagc gcgcagcgct tctacccgct cccggttcac
    14161 ctggaagtcg cgccggccca ggcgcgaggc ggagcgcacg tgcagcacgc ccgccccggc
    14221 gtcgaacgtg aattccacgt cgtccacgta acccatcagc ttggagcgga attccgcgtg
    14281 caggtagttg ccctcttcgc gcaccacgcg cgcgcgcggc atcgactcga cggcgcggcg
    14341 cgccgccgcg atcgcctccg cggcgcggcc cttgaacggt atcggcgtca catagcgttg
    14401 cgcatccccg gcatcggcct ggctggaaac gcaattcagc cgccggccgc aggaggcgag
    14461 cctgccgtcc tgtaccccga ggttgcccgg tgtcttgcct gcaaacacag tgtccccttc
    14521 cgcaatcgca agatgcaatg cgatcgccgc gccgagcact accgccagca gcactattcc
    14581 ggtgagcgcg cggcgctgca tggaagcgcc gatgctaaca tcggcgcagg atccgctcac
    14641 ggggagacac gcatggaaat ttccgcgcac cagctcgccg agctgctcat cggcatcgcc
    14701 cgcgcccagc acacgatcat ccagggcgta gagagcgcca tggccggcac caagacgcag
    14761 cacatcctgc cgatgctgca gaacgccgcg catctgcgcg atcacccgga gcccacgctc
    14821 gtcgacctgc cggtacgcgt cctgctctcg acgcagggcc gcgtgccgcc cgatccggcc
    14881 gcggtggcgc gcgacctgga gcgcctgctc gccgccgcgc ccgcggccta gaagtctttc
    14941 ggcggcggcg ggaagacggg ctttcgcttc tcgcggtgcg aggcggcgcc ctcgcgcacc
    15001 tccggaccgg tgaatcccag cagctccagc gccgtcgacg tatcgaacgt ggggcccgcc
    15061 atgcgcagcc agttgttcag gctgtacttg gtccagcgca gcgccgtctg cgcgccggcg
    15121 gcgagcttgc gcgccacctc cagcgccttc gcctgcagct cggcttcttc gcaggtgagc
    15181 gacacgaggc cgatgcgctc ggcctcctcg cccgagatct gctcgcaggt gagcaggtag
    15241 tacttcgcct tcgccatgcc gcagagcagc ggccagacga tcgccgcgtg gtcgcccgcg
    15301 gccacgccga gtcgcgtgtg gccgtcgatg atgcgcgcgt tcttcgccgc gatcgaaatg
    15361 tcggcgagca gccccgccac caggcccgcc cccacggccg ggccgtgcat cgccgagacc
    15421 acgatcttgg agcagttgat gatgttgtag accaggtcgc gcgcttcgcg ccagacgcgc
    15481 gcgcgcgtgt cgaagtcctt gatgatgtcc tcgatgaggc cgaagtcgcc gccggcggac
    15541 aagcccttgc ccgcgccgcg caggatggcg acgctcactt cggcgtcgcg gtcgacgtcg
    15601 cgccagatct cggcgagctc cgcgtgcatg cgcgcgcctg cggtggagag ccggccgctc
    15661 gcctcatcgc ccatcacgat ctcgaggatg ccgggctcga gccggcggat cttcagatcc
    15721 tggtatctag agtagtccat gcaggcattc tacaatttgg ccatgcgcac gcccgacgag
    15781 cgtttcgcgg atcttcccgg ctttccctgg gcgccgcact acctgcagtg gcgcgggctg
    15841 cgcgtgcact acgtcgacga aggccagggc gggcgcacct tcctttgcct gcacggcgag
    15901 ccgacctggg cctacttgta ccggcgcatg atcccgccgt ttgtcgccgg cggcgcgcgc
    15961 gtggtggcgc ccgacttcat cggctttggc cgctctgaca agcctgagga cgacgccttc
    16021 tatagcttcg acatgcaccg cgcgttcctc atcgaattcg tcgagcggct ggaactcagg
    16081 aacctcacct tggtagtgca agactggggc ggcctgctcg ggctgacgct gccgatggag
    16141 atgcccgagc gcttcgagcg cctgctcatc atgaacaccg cgctcggcac gggcgatgcg
    16201 cccctgcccg agggcttcct cgcctggcgc gcgtacgtgg caaagaaccc ggcgctcgac
    16261 tgccagaagc tcatgcagcg cagttgcccg cacctgacgc cgacggaggc cgcggcctac
    16321 gaggcgccgt tccccgatgc cagggcgaag gccggggtgc gccgctttcc gcagctggtg
    16381 cccgaccggc ccgacgcgcc cggcgctgag ctgtcgcgca aggcgcgcga ctggtggaag
    16441 agcgcctggc agggcgagtc gttcatggcg atcggcgcca aggatccggt gctcggcccg
    16501 cccgtgatgc gcgccctgcg caacgtgatc cggaactgtc ccgagcccta cgtgcacgcc
    16561 gagggcgggc atttcctgca ggagtggggc gaggacgtcg cggccagggc gctcgcgtca
    16621 tggcgctgac ctacgccgac gtcgccgccg cgcacgagcg catcagggct gcggcaaagc
    16681 gaacgccggt gctgacgtcg gccaccatcg atgcgcgcac gggtgcgcgc gtgcacttca
    16741 agtgcgagaa cttccagagg atgggcgcgt tcaagttccg cggcgcctac aacgccttgt
    16801 cacagctctc gcccgagcag aagaagaaag gcgtggtggc cttctcctcc ggcaaccacg
    16861 cgcaggcggt ggctctcgcc ggcaggctgc tcggcgtgcc ggcgaccatc gtcatgccta
    16921 ccgacgcgcc gaaggtaaag ctcgaggcca cgcgcggcta tggcgccgac atcgtgctgt
    16981 acgacgcgca caacgaagac cggcagatga tcgcggcgaa gctttccgcg gagcgcggcg
    17041 cgatgctgat tccgccgttc gaccacccgc acatcgctgc cggacagggc acggcggcaa
    17101 aggagctgat cgaagatgcg gggccgctgg acctgctctt ggtgccgtgc ggcggcggcg
    17161 ggctgctctc gggctgcgcg gtagcggcga agcacctgtc gcccgcgtgc cgggtgatcg
    17221 gcgtggagcc ggcggcgggc gacgacggcg cgcgctcgtt ccgctcgaag aagctgcagg
    17281 tgatcaagac gccggtcacc atcgccgacg gcgcgcgcac cgcgtcgctc ggaccgaacg
    17341 tcaccttccc ggccatcctc cggcacgtgg acgacttcct caccgtgacc gacgcggcgc
    17401 tgctcgaggc gatgttcttt ctgtgggagc gcatgaagat cgtggtcgag cctacgggcg
    17461 cgctggcggc ctgcgcgctg ctggagggca agctggacgc aagggggaag aaggtgggcg
    17521 tcgtgatctc gggcggcaac gttgatctcg cctgggcggc agcgcgcctg caggcccgct
    17581 gacttgcgcc ggctgcgcgt cctcgcgctc ctcgcctcgg cggcaatgcc ggcggccgcc
    17641 caggatccgt ttccctcgtt cgaggcgctc gaggccgcgg gcgccgtcat cggcgagatc
    17701 cgcatccgcg cgcaagacgt attcgatctt gacgacccgg aggaggacaa ctggttgttc
    17761 cgcaaggcca acgctctgca catccagacg cggccctcga ccctgcgccg ccagctgctg
    17821 ttccgcagcg gcgagcgcgt gtcggcgcgc gtgatcgccg agaccgagcg cctgctgcgc
    17881 ggcaacgact acctctaccg ggtcgagatc cggcccaccg gctacgatga tggcatcgtc
    17941 gatctcgagg tcgtcacgcg cgacagctgg acgctgcagc cggccgcgaa ggtcagcctg
    18001 gcggggggca cctcgagcgg cggcgtgagc ctggaggaga aaaacctcct cggtaccggc
    18061 gcgaggtttg cgatagcggc gcgcaccaca tcggaggtca gtaccgccgg aggcacgcgc
    18121 cgcggcgtgg gcgtggatct gtcctatccc tacgcgttcg acggtcgtac gacgctcggc
    18181 tacggtcagt cgaccttcga agagggctcg gcgcgcaagg cgaggatcga ccgccccttc
    18241 tactccctcg aggcccgctg ggccgccggc gccgcggcgg cgaggagcga tcaaattgtc
    18301 gaccgctacg cgaacggcgt cgtggcggcg cgctaccgcc agagcgtcga tagcgggcac
    18361 gcgctgctcg gtgtctcgcg cgggctagtg cggggttggg cgcatcgctt cagcggcggg
    18421 ctacgctacc aggaagagac ctaccgcctc gagcccggac aggcggcgcc cgcgacgctg
    18481 ccggccgatc gcacgctggt ggcgccattc ttgcgctacg aagcggtgca ggacaacttc
    18541 gtcgagctgc gcaacctgaa cagcattgcg cgcccggaat acctcgcgct cggctggcac
    18601 gcttggattg agatcggccg cgccgcgggc ggcctgggat cgactcgatc ggcgacgctc
    18661 tactcggcga gcctcgccaa gggcacgcgg atcggcggcg agggtacctt gctcgccgcg
    18721 gccgcggtcg ggggcgagta cgcctcgggc gaaaccgacc gcgagacact cggcgcctcc
    18781 ttgcgctact accagcgccg cggcgcccac acgctgctct ttctctccct tgctgccgat
    18841 gccaccgact tctccgaggc aacccaattc ctgtcactcg gcggcgaggt tggcgcgcgc
    18901 ggctttccca ccaactacca gcggggcgat cgccgcgtga cgttcacggc cgagcgtcgc
    18961 ttttactcgg attggttccc ttttcgcctc atccgcgtcg gcggtgccgt attcttcgat
    19021 gctggccgcg cctggggcgg cccgtaccaa aaactcgatg aagcggcggc gcactgggcc
    19081 accggtatcg gcttcgggct acgcctgctc agcgcgcgct cctcgagcgg caccaccgtg
    19141 cacgtagatc tcgccttccc cctgaagcgc gcgtccggcg tggacgcgtt ccaggtgtcg
    19201 gtgcagtcga aaaccgggtt ctagcccgcc gcgcccgcgc cgtaataggc gtagcgccgg
    19261 tccttctccg ccttcgacgc gtacatcgcc ttgtcggcga gcgcgatcag cgcctcggcc
    19321 gtctccgcat cgtccgggta cacgctgatg ccgatggagc acgacacctg cgccgccccg
    19381 ccctcgatgg cgagcggcac ctggatcatg tcggccacct tttcggcaat catcgcggcg
    19441 tcctcgccgc ggtcgagctc gcccgccagc agcacgaact cgtcgccgcc gtagcgcgcc
    19501 accgtgtccg agccgcggac gctggtgcgc aggcgcgcgg cgagctgcac cagcgcctgg
    19561 tcccccgcgg gatgcccccg ggtgtcgttg atggtcttga agtcgtcgag gtcgaccagc
    19621 attaccgcca gcttcttgcg gttccgcttg gccagctcca tcgccagcgc gagccgctgc
    19681 tgcatcgaca gccggttcgg cagccccgtg agcgcatcgt gctgcgccat gaagcgcagg
    19741 tgctgctcct gcgcctgcag ctgcgccatc agggcctcgc gctcgctcgc ctcggcgcgc
    19801 aggcgctcgt tgacgctgtt cagctcggcg gtgcgctcgg caatcctctg ctccagctcg
    19861 cgctcgctgt cgcgcgtcgc ctcgagcagc aaccgttgcg cggcgagcgc ctcgacctca
    19921 gcgctggcgc gctcgcggcg cagcaggttg atgcgatcac ccagcgcgaa cgacagcagc
    19981 agcacgtcca gggcgaagcc gatgtgcagc ccgtagatgg tcgcgaagct ggtcggcacc
    20041 agcgtgaagt tgcgcagggc gccgagggcg atgaaggcaa gcagcgccgc ccaggccagc
    20101 aggaagtagc gcgccgggcg atagccgaga atcagcgcgc gcaccgcggc gtaggtggcg
    20161 accgcggccg ccgcaaaggc gatcaccgag atggcgcgca gcacgccgcc gtggtcgagc
    20221 acccagccgg tgcccgcgaa cagcgccagc gccacgcccg ccgccacgac gccgcgcatc
    20281 accgcgtccg cgcgcggcgt tatgcgccgc atgtcgagga atgtgcgcga gaactgggcc
    20341 ccgaacatga gcgtgagcga cagcgcgagc gccagcacgt ggttcgcggc ccacacgctc
    20401 tgcggccaca ggtactggaa gcccaggccg tcgaaggaca gcaggtaggt gcagaacgcg
    20461 gtcgcgtaca gcacgtagaa caggtagacg cggtcgcgta ccgcgaacag cagcatcagg
    20521 ttgtacagca cgagcgccag cgccagcccg tagaacagcc cgagcaccag ttgtgcattg
    20581 cgtttgtgct cggcaaacgc cgccgggcgc cagagcgtca gcggcatggt cagcacgccg
    20641 cgcgactccg cacgaaggta gtaggtgtgc tcccccgcgg gcccaggcgc cgggaagcga
    20701 aacacgtgat tgcggtggcg cacttcgcgc gcgtcccaag gcagcgtgtc gccggcgata
    20761 cgcgcccaat aacgcgcctc gccgccggac agcggttcgg gcacgtagag ctcgagccgg
    20821 tcgatgctcg gatacgcgac ctcgagcagc agctcctcgc ccggcggcgc gccgccgggc
    20881 agcgagaagc gcagccacca ggccgagcgc gtgtaaccga agttgagcga accggcgccc
    20941 gccggcgcga agcgcgcggc gtgctcgggc atgcgcacgt catcgagcgc gaggccgccg
    21001 ccgggatcct cgagcacatc cacgtgcgcg ccgagcgcca cgcggccctc gacgtcgggc
    21061 agcggcgccg gcttgtgcgc gtgcgcggac gcgaaggtaa ggactgccag cagggcgagg
    21121 gcgcgcacca ttgggaacgg ctgaatcata ttgcagtgcc gcccgtgcgt taagatgtac
    21181 gccaccatgc gtgccatcca gatcgaaaag gccggcggtc ccgaagtgct gcgccccacg
    21241 gagcgagcgc ggcccgagcc caagccccac gagatcctcg tcaaggtggc cgccgccggc
    21301 gtgaaccgcc ccgatgtgct gcagcgccag ggactgtatg cgccgccgcc cgacgcctcg
    21361 gacctgcccg gactggaggt cgccggcgag gtcgcggcct gcggcgctgc ggtaacgatg
    21421 tggaaacctg gggacaaggt ttgcgcgctc acccacggcg gcggctacgc cgaatattgc
    21481 gtaacgcccg aagtccaggc gctgccggtg cccaagggcc tgtcgatggt cgaagcggcc
    21541 tcgctgcccg aaaccttctt caccgtctgg tcgaacgtct acgaccgcgg ccgtctcgcg
    21601 cccggcgaga cgctgctcgt gcacggcggc acctcgggca tcggcgtcac cgccatccag
    21661 atggcggcgg cgaccggcaa ccgcgtcttc gccaccgcgg gcaccgacga gaaatgcgcc
    21721 gcctgcctga agctcggcgc cgcccaggcc ttcaactaca ggacgcagga cttcgtcgaa
    21781 ggcgtgaagc aggcgaccgg cggcaagggc gtcgacgtga tcctcgacat ggcggcgggc
    21841 gatttcgtgc cgaaggagct caaggcgctc gccgacgaag gacgtctggt gttcatcgcc
    21901 ttcctgcgcg gccccaagac cgagctcgac gtgaacgagc tcatgcgccg gcgcctcacg
    21961 ctcaccggtt cgacgctgcg cccgcgcccg gtggcattca agggtgccat cgccgcgcgc
    22021 ctgcgcgagc gcatctggcc gctcgtcgaa gcgggcaaga tcaagccggt ggtcttcaag
    22081 accttcaagc tcgaggccgc cgccgaggcg caccggctga tggagtcgag cgcgcacacc
    22141 ggcaagctcg tccttaccgt ctagcttctt ccttcagcca ggcgatgaag tcggccacct
    22201 ccggccgccc ttgcgctgtc ggcgagacca ccgcgtaata ggcgcgcgcc ggatcggcgc
    22261 tgcgcttgaa cggcgccacc agctcgcccg aggcgagcgc gtccttcagg agcggcgtcc
    22321 tgccgagcgc gacgccgtgg ccggcgaccg ccgcggcgat gatctgctcg tagcccgaga
    22381 agctgatgcg gcccgcgggc ttgagctcgg acagcccctc cacctcgagc cacgagcgcc
    22441 agtgcagcca cgggtggcgc acgtccggat cgtcgtaggt cagcagcaca tggtggcgca
    22501 ggtcggcggg cgccttcagc ggccgcgcct tgtcctgcag cagcttcgga ttgcacaccg
    22561 ggaacacgcg ctcgccgaac aggcgctccg cgtccgggcc ggcgaggctc ggcgggccgt
    22621 ggcggatggc gacgtcgagc ccgtcgcgct cgagatcctg cacgcgcgtg tcggccgaga
    22681 tgcgcacgtc gacggccgga tgcgcgcgcg tgaagcccgc gaggcgcggg atcagccaca
    22741 gcgcggcaaa ggaatgcgtg gtggtgacgg cgagcgcgcg ccgcccgctc tgcgcgcgca
    22801 ggcggtcggt ggccgcgcgc atcgtcgcca gcacctgcgc cgcggtcgcg tagaactgcc
    22861 ggcccgcttc ggtgagcgcg agcgagcggt gccggcgctc gaacagcggc gtgccgagct
    22921 gcgtttcgag ctcctggatc tggcggctca cggccgactg ggtgaggtgg agctcctcgc
    22981 cggcgcgcgt gaaggagagc agccgggccg ccgcctcgaa gccgcgcagc aggtcgagcg
    23041 acggcagtct cgtattcccg ttactcatgc ttgtcccgac tttttaccgt ttgagcgctc
    23101 gggcccaagg atgcatcatg cgcatgccaa acacaaggag gtgccgcatg cgaatcgaac
    23161 tggatcccgg cgccctgcgg ctcgcccgcg ggcagacgtt gagagtgatc gacgcccccg
    23221 gcagcaccat ctgcgtgcgc gaaggcagcg tctggatcac ggaagagaac atgcccaagg
    23281 atgtggtcct agaggccggc cactgctacc ggctgagccg caaggggctc gccctggtgc
    23341 acgcctttgg cgatgccgcc gtggcgctcg cctgaactcc cgcaaggagg ctgtcgtgat
    23401 cgatacctgg gatctttccg cgcagcgcgc cgcagccgtg cacgcgctgc tcgcccgatt
    23461 cgcagcccgg ctgcgacgcg cgtctacaat aagcgctccg caagccaggg agcgcgaatg
    23521 tttcgggacg gattgctgaa gggcaagaac atcctcgtca ccgggggcgg caccggcctg
    23581 ggcaaggaga tcgccgccaa gtatttgcag ctgggcgccg gcctgtggat cgcgggccgg
    23641 cgcgccgggg ttctcgaaga caccgcgaag gagcttaccg cgaagcacgg cggcaccgtg
    23701 cgcacgcacg cggtggacat ccgcgactac gccgcggtcg acgccatgat ccagcacatc
    23761 tgggacgcgt cgggcccgct cacggggctc gtcaacaacg cagccggcaa cttcatcagt
    23821 ccgaccaagg acctcacgcc gaacggcttc aacgccatcg ccaacatcgt gttccacggc
    23881 accttctacg tcacgcactc gatcggcaag cgctggattg ccggcgggca caagggctcg
    23941 gtggtgtcga tcctggtgac ctgggtgtgg accggctcgc cctacgtcgt gccttcggcc
    24001 atgtccaagg cggggctgca catcatgacc aagtcgctcg ccgtggaatg gggcaagcac
    24061 ggcatccgcc tgaacgccat cgcgccgggg cccttcccca ccgagggcgc gagcaagcgc
    24121 ctcaggcccg gatcgagctt cggcgagtcg accaaggtga acccgatgcg ccgcgtcggc
    24181 gagatgtcgg agctgcagaa cctcgccgcg ttcctgatgg ccgacggctg cgagtggctc
    24241 tcgggcgaaa ccatcgccat cgacggcgcg ggctacctcg ccaccggcgc ctacttcacc
    24301 gagctcgaca agatgacgga cgccgactgg caaaaggcgc gcgagctgat caaggcgcag
    24361 aacgacaagg accgcgccgg ccggagcgcc tgagcgcgcg catgaccgac aagctcgtcc
    24421 cccgccccgc ggcgaccctg ctcctgctgc gcgatggcag cctcggtccc gaggcgttcc
    24481 tgctgcagcg cacgcacgac gccgcgttcc tcggcggcgc gcacgtgttc ccgggcggcg
    24541 cgctcgacaa ggccgaccgc gacccgcgcg tactgcgccg cgtcgccggg ctcaccgacg
    24601 ccgaggcgag cgcgcggctc ggcgtggacg ccggcgggct cgccttctgg gtggcggcga
    24661 tgcgcgaatg cttcgaggaa gcgggcatcc tgctcgccga gggcgaggac ggacggccgc
    24721 tcaccgcggc gcgcgcggtg gccctcgcgc ccagccgcgc actgctgcac tcgggcaagc
    24781 tcggcttcca cgagttcctc gagaaggagc ggctgctgct gcgcgggcgc gagctcgcct
    24841 acttcggcca ctgggtcacg gcgccggggc gcgcccggcg cttcgatacg cgctttttcc
    24901 ttgcgctcgc ccccgagacg cagcgcggcg cgcatgacgg caccgagctg gtggcgagcg
    24961 cgtggctgcg cccgcaggag gccatcgagc gcgaggcgcg cggcgagatc cagctggtct
    25021 tcgccaccaa gaacacgctc gccgacctgg tgcgcttcgc gcgcgccaag gacgcgctcg
    25081 agcacgcgcg cgaagccgag gtcgagacca accgcgcctg ctgggcactc gcgcccgacg
    25141 gcgccgcggc gctgttccgc cgccgcgacc cgcagtactt cgagatccac tggtccgatc
    25201 ccgaggaaac cgggcagacc agcgtcgcgc tcgcgcccgg gctcgccaag cggctcgatc
    25261 cgctggtgac gcgcgtgatc gcgcccaacc ccggcatgat gaccggcccc ggcaccaata
    25321 cctatctggt gggcgaaagc gagctcgcgg tcatcgaccc cggcccggcg atcgacgagc
    25381 acatcgccgc ggtgctcgcg gcgggcgcgg gccgcatccg ctggatcctg tgcacccaca
    25441 cgcacatgga ccattcgccc gcggccgccg cgctcaaggc cgccaccggc gccgtagtca
    25501 tcgggcgccc cgcgccggat caccccggcc aggaccgcga tttcgcgccg gagcgcgtgc
    25561 tcgcgcacgg cgatcgcttg atgttcggcg gattcacgtt gcgcgcgctg catacgccgg
    25621 gccatgcgtc gaaccacctg tgctacctgc tcgagaacac gcggatgctg ttcacgggcg
    25681 accacgtcat gcagggctcg acggtggtga tcaatccgcc cgacggcgac atgcgcgcct
    25741 acctcgcgtc gctggaactg ctgctcggcg aggacatcct gatcctcgcg ccgggccacg
    25801 gctacctgat cggcgagccg caccgcgagg cgcggcgcct gatccggcat cgcctggcgc
    25861 gcgagcagaa agtggtgtgg gcgctgcagc gtctcgccgc gcccactgcc gaggcgctgg
    25921 tgagcgaggt gtacgacgac gtcgcgccgc gcctgcacgg cgtggcgctg cgttcgctgt
    25981 cggcgcacct ggacaagctc gcgctcgaag gccgcgcgcg cgccgcggac gggcgctggt
    26041 cgctggtaca atcgtccacg gtcgagtgac cgccggggga gccgcgccat gggcagctat
    26101 gaacagacgc tgaagctggt caccaacctc gatcgcgccg cgatcgaagc gcgcctcgcc
    26161 gacgtccgcg gcactgcgca gtcggcgggc ctcgccgagg tggcgcagca gctcgtgcac
    26221 gcgcccggcg cgccgcgcgg cgacctcgag cagaggatcc acggcgcgct caagctcctc
    26281 gccggcaagc ccgagcacaa ggcgctccag gcgcagctcg agctggtgga gatcaacctt
    26341 ccgaacctga agtagcgacg cacccgcagc ctgaagttcg acttcgatct ctttaccatc
    26401 ggcggtggct cgggcggcgt gcgcgcgagc cgcgtggcgg cgtcattcgg cgctcgcgtc
    26461 gcggtggccg agtcgggccg cttcggcggc acctgcgtca acgtcggctg catcccgaag
    26521 aagctcttgt cctatgccgc gcactaccgc gaggacttcg cggtggcgaa gagcttcggc
    26581 tggagcgtcg gcgagccgac gttcgactgg cgcacgctgg tcgccaacaa ggaccgcgag
    26641 atcgcgcggc tgaacggcgt ctacgagaac gtcctgacga aggcgggcgc gaagatcctg
    26701 cgcgcgcgcg ccacggtgat cgacccgcat acggtcgaag cggacggccg gcgccacacc
    26761 gcacgccaca tcctcgtcgc caccggcagc cggccgcagg tgccgcagat tcccgggcgc
    26821 gagctcgcca tcacgtcgaa cgaggccttc tttctcgagc ggctgccgcg gcgcgcgctg
    26881 gtggtgggcg cgggctacat cgcgctcgag ttcgcttcca tcttcaacgg gctcggcgtg
    26941 cacacgacgc tcgcctaccg cggcgcgcgg ctgctgcgcg gcttcgacgc ggatctcggc
    27001 gagcgcatcg ccgaggagat gaggaagaaa ggcatcgccg tccgcctgca caccaatccc
    27061 gcgaagctgg aatccggcat ccgcgtcact tatgaagacg gtgccacgca ggaggtggac
    27121 ctcgtcctgt tcgccaccgg ccgcaaaccc aataccgcgg gcctcggcct ggaaggcgcc
    27181 ggcgtgcagc tcggcgcggg cggggcagtg gcggtagacg agttttcgcg cagctcggtg
    27241 gcctcgatcc acgccatcgg cgacgtcacc gatcgcctga atctcacgcc ggtggccacg
    27301 gccgaaggca tggcgctcgc gaagacgctg tttcgcggcg agcccacgcc ggtcgatcac
    27361 gtcaacgtgc ccaccgcggt cttctccgat ccgaatctcg ccacagtggg gttgtcggag
    27421 gaagcagcgc gccggcattg cagtgaagtg gacgtctaca agacggcgtt ccgtgccctg
    27481 aagctcaccc tgggcgggca cgaggagcgc acgttcatga agctggtggt cgatgctgcg
    27541 tcgcagcggg tggtcggcgc gcacatgatc gggccggagg ccggcgagat aattcaaggc
    27601 gttgcgatcg ccgtgaagct gggcgcgacg aaagcccagt tcgatgccac tatcggcatc
    27661 caccctaccg cggcggagga attcgttacc atgcgggaga aggcgtcctg acaatggttc
    27721 tcgaatcgca gatcgagaag atggttggcc agttgcgcgc cagcgccggc ctgccgctcg
    27781 ccatcgagct gtggaacggc aagcgctacg ccctgggcga gcgcccgacc gtgacgctgc
    27841 gggtccccgc cgcctcggcg gtgaagtacc tggcgaaagc cgacctcgcc gcgctcggcg
    27901 aggccttcgt cgagggccac atcgaggtcg acgggcccat cggcgacgcc atccgcgcgg
    27961 ccgaaagcct ggcgcggcgc ctgggcggcg ccaggaaggg ccgcgtcccc ggcctgctgc
    28021 gcaacatgca ctcgaagaag accgattcgg cggcgatccg ctaccactac gacgtctcga
    28081 acgactttta cgcgctgtgg ctggacaggc gcatggtcta ttcctgcgcc tacttccgga
    28141 ccggcgaaga atcgatcgag caggcgcagg agcacaagct cgaccacatc tgccgcaagc
    28201 tcaggctggc gccgggcgag cgctttctcg atatcggctg cggctggggc gcgctcgtgc
    28261 tgcacgccgc cgagcgctac ggcgtgcgcg cgaccggcat cacgctctcg gagaaccagc
    28321 acgcgctcgc caatgagcgc atccgcgccg cgggcctgca ggaccggtgc gaggtgcggc
    28381 tgcaggacta ccgcgacgtg cccggcgagg gcgcgttcga caagatcgcc tcggtgggca
    28441 tgttcgagca cgtcggcctg aagaacctgc cgctgtactt cggcgtcatt cgccggctgc
    28501 tggccgacgg cggcatcgcc ctcaaccacg gcattacgtc catcgatccg gacagccgct
    28561 ccgtgggcct gggcgcgggc gacttcatcg gcaagtacgt cttcccgcac ggcgagctgc
    28621 cgcacctgtc gctcgtgatc cgcgagatgg gcgccgccgg cctcgaggtc atggactcgg
    28681 agacgctgcg gctgcattac gccaagacgc tgcagcactg gagcgggcgg ctggaagcga
    28741 acctggacca ggcgcgcaag ttcgccggcg agaagcgcct gcgcatctgg cgcgcgtacc
    28801 ttgcgggctg cgcgcacgcg ttcggcgagg gctgggtgtc gatccagcag gtgctggcgg
    28861 tgaagacggg cagcgatccg cgccgcaacc cgctgccgtg gacgcgggac tacatgtacc
    28921 agcgctagcc cagcgcgcgc aggaccaggc tgccccccgt caacagcagc accgccgcca
    28981 ccgcgcgcag cagctgctcg cgcgagatcc tgaggtacat gcgccgcgcc accgcgatgc
    29041 ccagcatcgc cgcaggcacc gcgccgatcg ccaccagcca caccttcggc tcgagcagca
    29101 ggccggtgag cagaaacgcg gctacgcgca gcgagatgct ggtcagcgtt gcaaagccca
    29161 tcgtcgcgcg gaactgctcc ttggtgagcc cgcgctgcga cagatagatc gcgtagggcg
    29221 gcccgcccgc accgaacagc gtgctggtga cgccgccggc gagcccggcg atccatgccc
    29281 agcccgccgc gatcacgcgg tgcgactcat gccggaacag gctgtagagc gcgtagccga
    29341 agacgaacgc gccgagcgcg gccatgcccg ccgcgcgcgg caggttgacc aggaccgtga
    29401 cgccgagcgc cgtgccggcg aggatcatcg gcacgaggcg catccactcc gccttcaccg
    29461 cgtttctcgg gctctcgaag cccacgcgca gcgcgctcga caggtcgcac agcgcgtaca
    29521 gcgccagcgc cagctgcagg ggcacgaaat gcgtggcgag cggcatggtg accagcgccg
    29581 agccaaagcc ggtgagcccg aaaaccaggg caccggcaaa cgcgatcggg atgagggcga
    29641 gcagctccca ctgcccgccg aggagctcgc tcaggccttc accgccgggt tgagcgtgta
    29701 gaggcccgtg atcttggcgc tgccgagcac gtgcggctgg atcgccttga ggagctcggc
    29761 gccgcggaac gcgccctcca gcgggcaggt gtccacgccg agcgcataca gcgtgaagac
    29821 gtagcggtgc acgatcgagt cgttccaggg cgggcagggc ccgtcgtagc cgaagtactc
    29881 gcccgccatg tccttgtcgg atgcgaacca gctggtgtag tcgttcacgc cctggcgcgt
    29941 gccgcgcggc cccgccgggc cggacttgcc gcccgcggtg acgccgctcg agaactcgcc
    30001 ctcgcggatc ggcccggcgt cgggcttgag atccaccagc agccagtgga agaactcgat
    30061 gcgcggcagc gtcgccggga tctggcggtc ctccttgttg acgtcgtcgg gcttcgatgg
    30121 cacgtcgacg tcgccgcaca ccagaacgag cgacttcgtg cccgccggca ggtccctcca
    30181 ggcgaagtgc ggattgcggt tgtcggagag cttgacgtgg gtcttggcgt ccgccacgcc
    30241 aaacgcgcac ttgccgggga tcgcgccctt gtcgctgaag ctgtcgctcg agagaatcat
    30301 gcgccgcctc cttcggggac aattataaga gccccggcgt cgaaccggtt catgcgtttc
    30361 agtcgcggca cgtagcgcgg gaacggcagg ccgaagcgcc gctcgagccc gtgcgcgcgg
    30421 ctcgcgagcg tgtcccagga cagcgcgcgc ggcgcgccct tgagcgcgat gacgatcacg
    30481 ttcgggtcat agagcgcggg cagcgccagc actgcgccgc cgaacgcgcg ctcgacgcgc
    30541 gcgatgcgct catcgagctt cggatcgtcg ttcatcagat tgagcaccag cgcacccggc
    30601 gactcgagcg cgagccaggc cgcgtcgaag aacgcctggc tgacgagcgc ggcgggcggg
    30661 tgctcgtcgg cgaacgcgtc caccaccagc aggtcgcagc actcgggcgc gagcgcctcg
    30721 gcgccgtcgc cgatctccac cacgaggcgc gcgtcatccg ccggcaggtg gaactgcgca
    30781 cgcgccacct gcaccacgcg cgggtcgagc tccaccacgc gcgtgcgcag ccgcggcagg
    30841 cgccgccaga agaacttcac cagcgacccg ccccccagcc cgatcttcag cgcgcgcgcg
    30901 gggcgcgggt gaaaaagaag gaacgtcatc atgcagcgcg tgtactcgag ctcgagcgca
    30961 aacgggtccg acaggcgcat cgagctctgg atggcttcgc cgccgacgtg cagcgtgcgc
    31021 acgccgcgcg cctcgctcac cgtgagcgcc ggcacgcggc tcatgcgtgg cgctgcggca
    31081 ggtgccggcg catcagcacc gcgagcgtgg cgccggcgaa gctgagcgct gcgcaggcga
    31141 ggatcgacag gtcgtagctg ccggtggcgt caaagctcgc accggcgaac gtcggtccga
    31201 ccagcgtgcc caggcctgcc gcggtgtaca aggcgccgat gatggccgag agcgcgcgcg
    31261 ggccgaagat gtccatgcac acagtgggcg cgagcgccac atagccgcca tacagggtgc
    31321 cgaacaccac ggcgaatacc gccagcgcga ggtagctgct cgatgcccac cacagcacga
    31381 acatgagccc cagcccggcg ccgacgccca tcagcgcgcg caggtggccg agacggtcgg
    31441 caatgccgcc gacgaaaaag cgtccgagca ggcttcccag gcccaggagg ctcacgagcg
    31501 acacgccttg cgcttcgctc aggccgacgt cgatcgcgta gggcaccagg tggaccatgg
    31561 gcacgaagaa ccccacgcag ataagaagcg ccacgccgta cagcatccag aacaccgggc
    31621 tcctcaggcc ttcggcgagc gtcatgccgg gaagcggcgc gccgcccgcg ccgcggcgca
    31681 cggatttgcg cccgccgagc gcgagcgcag cgggcaccga gagcaggagc atcgccgcgg
    31741 cgagcgcgag gtacgcatgg cgccagccca ccagcccgat cagccaggct gccgccagcg
    31801 gcatgacgag gttgcccgcg ccgatgccgg acacggcgat gcccgaggcg agcgcgcgat
    31861 gctgcacgaa cgcctgctgc acggcggcga ccgagggcac gtaggaaagc ccgatgccca
    31921 gacccgcgcc gaggctgtaa gtcaggtaaa gcagctccac cgaaggcgcg ttggcggcga
    31981 gcgcgaggcc ggccgccagg cacagcgcgc ccgcgaggca cacgccgcgc ggcccgaagc
    32041 gatcggcgag catgccgccc ggcacgccgg ccgagaacca cagcagggcg ctcagcgaaa
    32101 aaaccagcga gacgtgggcg cgcgaggcgc cgaactcggc ttcgaaggcg gcgaagaacg
    32161 cggcgaacga ataggccgcg ccgaagccca tgaacatgag cgtgaaggcg gcgagcacca
    32221 ccccccagcc cggacgcggc atcgccaagt cagcgctgcg cgcgtgtgcg cgccgccggc
    32281 agcgccgcga tcacctcgac ctcgagcagc aggtcgtcgt gcaccagccg ctccacgccc
    32341 accagcgtgc tcgccggcat cgccgccgga tcgaggtagc gcgcgcgcac ctcgcggtac
    32401 tggttcacgg cctcggcgct caggcccacc atgtagccgt tcatcttgac cacgtcgcgc
    32461 caggtggcgc ccgcggcctt caggttgtgc gtgaggctcg cgaacacctg ctcggcctgc
    32521 gcgcgcatgt cgcccttgcc gacgacgatg ccgtcgcgat cgtagctcac ctgccccgac
    32581 acgaacacca gcgtcgccgc gcgctccagc gccaccgtgt gcgtgaagaa gcgcggatcg
    32641 aacaacgcct tcggattgag attgcgtcgc ttcatgtcag cgccccccgt gcagtaatcg
    32701 gccacaagga ctccaccacg cccttgcgca cgcaaacata ccagtcgtac aggttgatcg
    32761 tcgggtcgca gtgcccgggc acgaggagca gcctctcgcc gggcgcaggc gcggcagtcc
    32821 cgggcgcgac ctcgatgaag ccatgctcgt ccgaggcctg gacgtagctc aggcccgggc
    32881 gctgccacac cgcgggcatg ccggaatcga cgctcgaggc cttcaggccc gcatcgacga
    32941 tggcgcgcgc gccgccgccg cggctcatga cggtcgtcag cacgaacagc gcgtgcgcaa
    33001 agcgcggcaa cggcgccgac tgctcgtttc gcgcgtaatc cgcatccatg aacgcatacg
    33061 agcccggctg gatctcgttc caggcgcccg acgccacctc cagcatgaag gtgccgctgc
    33121 ccgccccggt caccgtcggg cattcgatgc cgttcttctc gagcagcgcc cgcgtggctc
    33181 gcacgtgctc gcccgcacgc tcgatgagcg cgcggcgctc ggcgatggag cgtacgtgct
    33241 gcgcgcgccc gttgtaggcc tgcaggccct gaaagcgcag gttcgagcag cccgccacca
    33301 cgcgcgccag cgcgagcgcg ggctcgcccg gcgccacgcc gcagcgcccc atgcccacct
    33361 cgagctcgat gtacacgtcc agcttggcgc cggcgcgggc ggcggcggca tcggccgcac
    33421 gcgcgttgtc cgcgtcgtct acgcacacgc cgatgcgggc gcggcccgcg aggcgcgcca
    33481 ggcgctccag cttctgtgcg ccgacgatct cgttggtcac cagcacgtcg gtgatgccgc
    33541 ccgccaccat ggcctcggcc tccgacacct tctggcagca ggcgcccacc gcgccgagcg
    33601 ccatctgccg ctttccgaca tccgggcact tgtgcgtctt ggcgtgcgtg cgcacgcgta
    33661 cgcccgtgcc gcgcaccgcg tcggccagcg tgcgcaggtt ggcctcgaac gcgtccagct
    33721 cgaggatcag cgccggggta tcgacctcct ccaggcgctg gccgatcgcg gcgggcggcc
    33781 tcatgcgaca cctgtcctgc ggtggtgggt catgccgtac cagacgatgg cgatggcgac
    33841 caccgcgatc atcggcagcg cgaacaggtt gacgcgctcc cagcccgccg cggtcaccgt
    33901 caggcccgag gtgaacgaag agatcgccat catgatgaag atcgcctggt cgttggcgcc
    33961 ctgtgccttc gccatttcct cggggcggta ggtctcggtg agcagcgccg tgccgccgat
    34021 gaacaggaaa ttccagccca caccgagcag cacta
//
LOCUS       DBNHKE010000160        34744 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_186137, whole genome shotgun sequence.
ACCESSION   DBNHKE010000160 DBNHKE010000000
VERSION     DBNHKE010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 34744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34744
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_186137"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(508..873)
                     /locus_tag="AC16O9_10775"
     CDS             complement(508..873)
                     /locus_tag="AC16O9_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517139.1"
                     /translation="MATMVFVLPAPVLAQSSSDWVDIKDPIELRALYSNKTFRGNGWV
                     GHYRADGKGIFISQGGKPQPRTWEVKGNDWVCVAPSDGPANCFRFQRNRKKPNELVVI
                     NVTSGATLNFTLEDGIPKF"
     gene            complement(1096..1752)
                     /locus_tag="AC16O9_10780"
     CDS             complement(1096..1752)
                     /locus_tag="AC16O9_10780"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6517140.1"
                     /translation="MNAMTAAEIVLYGPAYSTYTRTARLALEEKGVGYTLVEVDFLRG
                     ANKSPEHLLRHPFGKVPVLEHGRLKLFETAAILDYVDSAFAGATLQPKDARVRARMLQ
                     AIGVIVSYIYPAMVGQILVERLIKPVLGQPPDQSIVDSGLRRLRKGLAVLEGLVEAPS
                     FLIETSISLADLYLVPMWAYFVQTPEGKAVAAGLPRLASWWDAMSSRSSVIKTAVVLQ
                     "
     gene            1890..2933
                     /locus_tag="AC16O9_10785"
     CDS             1890..2933
                     /locus_tag="AC16O9_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR6517141.1"
                     /translation="MVQSSELPEVSSGSIAQPRLRDRFRGEIRKRGYSRRTEKSYWFW
                     IRWFIFHNGTRHPAELGAREVEAFLSWLAAERNVAAATQNQALAALLFLYKQVLGQDL
                     PWFSSMVRAKRPVRAPVVLTQAEAARLLEQLQGVKWLMASLLYGAGLRQIECLSLRVK
                     DLDFAYRQITVRDGKGGKDRVTMLPEQVVQPLQAHLGRVRALHRRDLAAGHGEASLPK
                     ALARKYPRAGREWAWQFVFPSRNPSADPESGVIRRHHLFPDTLQRAVKEAAKAARIVK
                     PVSCHTLRHSFATHLLEAGHDIRTVQEPLGHADVSTTMIYTHVLNKGGRGVKSPLDRL
                     EQPGTAYEPRTTG"
     gene            complement(3065..4279)
                     /locus_tag="AC16O9_10790"
     CDS             complement(3065..4279)
                     /locus_tag="AC16O9_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6517142.1"
                     /translation="MNAPLKSKPKARFSWEDPLLLDAQLTEDERMVRDAAAAYCRDKL
                     APRVLEAFREEKADLKVFREMGELGLLGPTIPSEYGGPGLSYVCYGLIAREVERIDSG
                     YRSTMSVQSSLVMLPIFEFGTEAQKKKYLPRLATGEYVGCFGLTEPNHGSDPGSMASR
                     AKKVKGGYSLTGSKMWISNSPVADVFVVWAKEVDEKGNVGEIRGFILDKGAKGLSAPK
                     IHGKVGLRTSVTGEIVMDDVFVPEENAFPEVRGLKGPFTCLNSARYGIAWGALGAAED
                     CFHRARQYVLDRKQFNRPLAANQLIQKKLADMQTEIALGLQACLRLGRMKDEGTASPE
                     ITSILKRNSCGKALDIARTARDMLGGNGISDEFGVIRHVVNLEVVNTYEGTHDIHALI
                     LGRAITGIQAFS"
     gene            complement(4338..5543)
                     /locus_tag="AC16O9_10795"
     CDS             complement(4338..5543)
                     /locus_tag="AC16O9_10795"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HIR6517143.1"
                     /translation="MLDAYIYDGIRSPIGRHAGKLAPMRPDDLAGEVIAEVVKRNGVK
                     PEDISDVILGCVTQAGEDCRNVARFAALMAGLPPTTPAVTVNRLCASGLQAVTDAARA
                     ITAGEGELYIAGGVESMSRAPYVFAKSETPYGRDVKLYDSTIGSRFTNPRFKKLFGDH
                     QMPETGDNVAKEFGVTREQADEFALASQKKYAKAESEGFYKGEIMAIALPQKKKDAPP
                     VKVEADEHPRRDSTMESLAKLKPLYEGGVVTAGNASGVNDGAAALIIASRKWGDRNGK
                     KPIARILSAASAAVEPRIMGVGPAYAIPRALERAGKSLKEMDIIEINEAFASQVLACL
                     NLMKLDNKDPRVNPNGGAIAIGHPLGCSGARLALTVARELQQSGGKLAAVSLCIGVGQ
                     GLAVILQRT"
     gene            complement(5548..7044)
                     /locus_tag="AC16O9_10800"
     CDS             complement(5548..7044)
                     /locus_tag="AC16O9_10800"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR6517144.1"
                     /translation="MLDPSRSDLLAGVAGSGTMGRGIVQVLAQCGARVRVFDAQAGAA
                     AKAKDAIAKALAGVVAKGRLAQADADAALARIEIAEAPAAFKDCHLVIEAIVEDLAVK
                     QKLLRELEAIVGDTCILASNTSSLSVTAMAAACARPGRVAGYHFFNPVPLMKIVEVVD
                     GELTEPWVGEALAGLAKRFGHTAVRCKDTPGFVVNHAGRGFVPESLRVLQEGVADFAT
                     IDRILVDAAGFRMGPFSLMDLVGLDVAHAVMKSMHQQYHGEPKYQPAYIAETRVAAGL
                     LGRKTGRGWYRYGKDNAPEKIPEQPVPQAKAASVWAAPELKDFAASLGAKLEAKPLAG
                     TLCLVAPLGADATTAALQAGLDPKHTVAIDPLFGFAKRRTLMPTPVTTREAREAAHGL
                     LAADGVPVSVIRDSAGFVAQRVVAHIVNVGCDIVQQRIATPEDLDRAVMLGLGYPKGP
                     LGLGDAVGPAKVLAILEAMHEFTKEPRYRPSPWLTRRARLGVSLLTPE"
     gene            7122..7562
                     /locus_tag="AC16O9_10805"
     CDS             7122..7562
                     /locus_tag="AC16O9_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6517145.1"
                     /translation="MKYRKILLAYNGSAEGKRALLECAELANFLGAETHLLAVASMPP
                     SLFLTEGFVPEELLEEEKKRTQTVLDEGIANLKDQGFQATGHLAVGEPVEEICRLAKE
                     LAVDLIVVGHNQNTSFAARWWKGSVGASILDYAPCSLLVALSRN"
     gene            7577..9901
                     /locus_tag="AC16O9_10810"
     CDS             7577..9901
                     /locus_tag="AC16O9_10810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6517146.1"
                     /translation="MESFGHQGRLEDERMLKGAGRYVSDWTLPGEARGCFVRSDRPHA
                     EIAGIDAKAALAMPGVLAVITGAELVQAGLKPIPAAAPFKWSDGSEQRQALRLSLAHG
                     KVRHVGEPVALVVAQTAAQAQDAAESVVVDYKDLPAAVTAEEALAKGAPQLHEIAPGN
                     LALDFTGGDEAAAKAAFEKAARVVKLKAYHTRVVGNPMEPRAAMGSYDPKTETYFLHA
                     TTQGVGPMRAQCSAVLGVPPEKVRVVAEEVGGGFGVRFNAYPEYGALLYAAKKLGRPV
                     KWVGTRSEVFLGDEQARDIVHVAEMALDAQGRILGMRFDYLANLGAYIAFTGAVVNTI
                     GMVSVICGVYDVQAIHVRGRLVLTNTVPTAAYRGAGRPVASYALERLVDQAAHELKMD
                     PAEFRRRNMIPKSKMPYKQVSGFEYDCGDFPGVLEQAKKEADWDGFDKRRAESKKRGK
                     LRGRGISAYIEMTAPGGFAPYDQVVVSWDKDGGVTLRTASHNHGQGHETVFAQITARV
                     LGIPMHKIRLKTSEPDTNLVANPTGGSRTAHGLGSAVLLAAREIVDKGMDLAADELEA
                     AKPDLEFKEGEYRIKGTDRKIAITALAQKHPGKLDLDFKERPKVPSTYPNGCHIAEVE
                     IEPETGEVEIVSYLACDDAGNLLNEQLVHGQMHGGITQGAGHIFGEQAIYDDSGQLLT
                     GSFMDYAMPRPGLVGGLRVTEHPVPTATNPLGAKGVGEAGVTGSMPCLMNAVMDALRQ
                     EGVTHFDMPASPLRVWRAIREAKAGNPAALAVKQ"
     gene            complement(10094..10951)
                     /locus_tag="AC16O9_10815"
     CDS             complement(10094..10951)
                     /locus_tag="AC16O9_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6517147.1"
                     /translation="MAYQQIRYDLAERVATLTLNRPDKLNAFTHVMRDELVDAFSKAD
                     ADDEVRAVIVTGAGRAFCAGADLSAGRETFDYARREGLSADTHRDGGGRVTLRIFEMK
                     KPVIAAVNGPAVGIGATMLLPMDVRLASSEARFGFVFTRRGIVPEACSSWFLPRLVGI
                     SQALEWTLSGRVFPAQEALAGKLVSKVVPPEELLPAARALAREIVEHTAPVSVALARQ
                     LMWRMLGADHPMEAHKVDSRGIFYMGASPDVAEGITAFKEKRAPDFGMRVSRDMPPFY
                     PWWEERKFK"
     gene            complement(11100..12440)
                     /gene="pcaB"
                     /locus_tag="AC16O9_10820"
     CDS             complement(11100..12440)
                     /gene="pcaB"
                     /locus_tag="AC16O9_10820"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HIR6517148.1"
                     /translation="MASTVLDSALFRDSFGTPAMRAIFEDAALLARYTEVEVALARAQ
                     GRLGVIPAQAAKDIAAKCDASKLDLERLKQETETVGYPILPLVRQLAAQCGEAGRYLH
                     WGATTQDIMDTAVVLQVRDALALIDKDLAELQSILARQAKRYRDTPMAGRTHLQQALP
                     VTFGYKLAIWLAMVDRHAERLQQLKPRVLVGQFAGAAGTLASLGDKGMAVQKAVMEEL
                     KLAQPVATWHAARDGLAEAVNFLGLVTGSLGKVAFDVMLMASTEVAEVSEPFVPGRGA
                     SSTMPQKRNPISSELILAAAKAVRQHAGLMLDALVQDFERATGPWHAEWIALPEAFML
                     SGGALHQAKFMLAGLIVDEARMRKNLDMTHGLIVAEAVMMGLAPHLGRNEAHDVVYDA
                     CRAVAEKGGSLAEALARVPEVTRHLDRQAIEKLTDPSNYLGAAREMVERVVGKR"
     gene            complement(12452..12784)
                     /locus_tag="AC16O9_10825"
     CDS             complement(12452..12784)
                     /locus_tag="AC16O9_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR6517149.1"
                     /translation="MRSKPVVPRELANRDVDEAIAYYLDQGAPQAAMGFIAALEKAYA
                     HLARFPQSGSPRYALELNLPGLRCWSLAGYPFLIFHLGLDDRVDVWRVLHGQRDVPAW
                     MQAPDTPS"
     gene            complement(12781..13032)
                     /locus_tag="AC16O9_10830"
     CDS             complement(12781..13032)
                     /locus_tag="AC16O9_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HIR6517150.1"
                     /translation="MSTMNISLPDALKDFVDEQVNSRGYGTSSEYVRELIRKDQERQT
                     LRGLLVAGAESRLADPADAAYFARLRRRVRGRGRSGNRR"
     gene            complement(13042..13824)
                     /locus_tag="AC16O9_10835"
     CDS             complement(13042..13824)
                     /locus_tag="AC16O9_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517151.1"
                     /translation="MPALLSADSVTELDARHRGAVLVAGSHGGRIAAYYASRAAVHAV
                     ILNDAGVGKDEAGVAGIAALESIGMAAAAADCATARIGDGTDTLARGVISRANRLAAA
                     CGVEPGMPVRDAAVRLTQAPAPRGTLASPGEGRFEVHAGIWALDSVGLARPQDAGKVL
                     IFGSHGALHGGRVETAIPVPARAAAFHDAGARQADMTRLPVLAARGIPAVTVATMSAR
                     IGEARSMWESGTISFANAPASGLGARPGQPLAAWLAKLCQAA"
     gene            complement(13799..14776)
                     /locus_tag="AC16O9_10840"
     CDS             complement(13799..14776)
                     /locus_tag="AC16O9_10840"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6517152.1"
                     /translation="MARMTYAQAACSALEAAMRGDDRVVVVGEDVGRGGIFGQYRGLQ
                     QSFGAKRVIDTPISESTILGAAVGMALAGLKPVVEMRVVDFALCAIDELVNQAAKARY
                     MFGGQGRVGMVARLPNGLWAGSAAQHSQSLEAWFAHIPGLVVVAPATPQDNHALLAAS
                     LASGDPVVYLEHKELWGLEGEVAPAGGAQLGEAEIAHPGSDVTVVTWSKTRHAALEAA
                     ALAEKDGVSLEVIDLRTIWPWDRDKVLASAERTGRVLIAHEAVQVAGFGAEIAATLAE
                     ESGARVRRLGAPRIPVGYSLPLEDESRITPQAIAAACVEMHRARPAQRG"
     gene            complement(14764..15771)
                     /locus_tag="AC16O9_10845"
     CDS             complement(14764..15771)
                     /locus_tag="AC16O9_10845"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6517153.1"
                     /translation="MEEAPDVGRALALYRTMARIRAFEDAAEDASMGGVAAFGASLAQ
                     AQVRGPLHLSTGQEAVAAGVCAHLARTDLLTSTHRGHGHTIAKGAQLDRMMCELFGRA
                     TGYNGGKGGSMHIADFSVGMLGANGVVAAGIPIAVGAAHALAMRGTRDIVACFFGDGA
                     INRGPFLEGLNWAAVHKLPILFICEDNRWAATTASDATTAGPGALARAAALGVPGSEV
                     DGNDVFSVWCAAGKLLAEVRNGDGPRLLHAVTYRVKGHVSVDVAGYRDAARHDKELER
                     DPLVLAAMRINMRETLEAIGREAREEVAAALKAAHEAPWPGKASAYSDVQDTGAGRWR
                     G"
     gene            15798..17003
                     /gene="phnA"
                     /locus_tag="AC16O9_10850"
     CDS             15798..17003
                     /gene="phnA"
                     /locus_tag="AC16O9_10850"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HIR6517154.1"
                     /translation="MPKRAPLTVNGRSYRWMDRPLAAICVDGCEFDYIHEAVAAGVAP
                     YLGRVLRSGAAFEADCVVPSFTNPNNLSIVTGVPPSVHGICGNYFYDREADAEVMMND
                     PKYLRVGTILAAFAKAGASVAVVTAKDKLRTLLGKDLDGTCFSAEKADQATQALVGKP
                     QPSVYSAELSEFVFAAGVKLMETRKPDLMYLSTTDYVQHKAAPGSPAANDFYAMMDRY
                     LARLDELGATIALTADHGMNDKFGADGQPNAVYLQDLLDEWLGAGRARVILPITDPYV
                     VHHGALGSFATVYLPSDANASAVIASLSKVDGVQMALTNADACARFELPPERLGDVIV
                     VADRHAVLGKGRAHHDLSGLDVPLRSHGGLSEQTVPLVFNRRTAGIPGKTRMRNFDVI
                     DVALNHLGA"
     gene            17009..17308
                     /locus_tag="AC16O9_10855"
     CDS             17009..17308
                     /locus_tag="AC16O9_10855"
                     /inference="COORDINATES: protein motif:HMM:NF045581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG0541 family transporter-associated protein"
                     /protein_id="HIR6517155.1"
                     /translation="MKMALVVCNSYFMDRVMKVLEDNGIDYFTSWDNAKGKGHGTEPH
                     LGTRAYGSTNSVTMIAFEDEAPLEGLIAGIKEENREIRRAPDHIRLFLLPLERIV"
     gene            complement(17343..18362)
                     /locus_tag="AC16O9_10860"
     CDS             complement(17343..18362)
                     /locus_tag="AC16O9_10860"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1 family esterase"
                     /protein_id="HIR6517156.1"
                     /translation="MRVFVPIQIVLLSIAVSACASAQPRTCTPANARPGAALTCDVPG
                     FERRPYDIHLPVDYVPARPVPVVLAIHGGGGNSGAAARTACPGGDVGSADCLHAMARR
                     EGAAVAYPNGTGARLARNVRTWNAGGGANGWQCVSGRACEDGVDDIAYFRALLADLSR
                     WVAVDPRRVYATGLSNGGAMSHRLACQLAERVAAIAAVGGANQFATVSACAPPRPVPV
                     LQIHGTADPCWRYEGGKAACAQRDDTAKISVEESMRRWAAINGCAAAGAPRPLGSEPR
                     VNTVQLAWTGCQADTVLIRMEGGGHVWPGGWAYFRERVIGPMVGGWSANRVILDFFRR
                     QTMPD"
     gene            complement(18408..19409)
                     /locus_tag="AC16O9_10865"
     CDS             complement(18408..19409)
                     /locus_tag="AC16O9_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6517157.1"
                     /translation="MDVLSDVLRKIRLHGALFLNGEFRAPWCLNVPTGADLAQVLQPG
                     AKQLAICHFVLEGQCWVELSGGEPTHLQQGDIVVYPHGDSHHMGSGLQIAAAHIDHVV
                     RPRMPELNPARYGGDGEQALLVCCWFAYERELPNPLVANLPSQFVTALGKRPSGQWIA
                     QSIRYALEQAAGHQAGFDVVAAKVAELLFVEALRGYMESLPPAQAGWLSGLRDPHIGK
                     CLSLLHADPARSWTVAELAQAVNLSRSVLAERFAELVKVSPMQYLKQWRLSMAAGMLK
                     HDRHSLGHIADSIGYESEAAFSRAFKQSYGISPGQWRRSERAPGSASDTAPIAAAAN"
     gene            19494..20360
                     /locus_tag="AC16O9_10870"
     CDS             19494..20360
                     /locus_tag="AC16O9_10870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6517158.1"
                     /translation="MKAAKSINPSSTAQGEAMNPNKALWEKGDFTRIAEGMRESGEAV
                     ARTFGIRPGLKVLDVGCGDGTTALPAAQQGAEVLGVDIAKNLVAAGNQRAAAAGLTNC
                     RFQEGDVSDMRELADDSFDLVVSIFGAMFAPRPFDVAKEMARVARPGGRVIMGNWIPN
                     DPTLVAQILKISSAYTPPPPEGFVSPMTWGVEDKVIERFAAAGVSAQQISFARETFTF
                     KTSGAPSAYLQAFKTYYGPTMNAFEAAEKNGRAADLQGELEELFKSQNKSPRADATSI
                     PATFLRVTVALQ"
     gene            20373..20819
                     /locus_tag="AC16O9_10875"
     CDS             20373..20819
                     /locus_tag="AC16O9_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517159.1"
                     /translation="MFMIDYRQRGWWYWLLTACLLTGGVAGYPFGFELAIGLTVVHMI
                     DYIVRERSLIAFTVQVRLSVLLYLLLSYPEPLQFLIWVAVVGIWARALFGYCLMARTL
                     SLMPWNRRVPFTGALLFKTYFSRPVRGNIMQGLAPLAPALGAATGG"
     gene            complement(20861..21228)
                     /gene="ssrA"
                     /locus_tag="AC16O9_10880"
     tmRNA           complement(20861..21228)
                     /gene="ssrA"
                     /locus_tag="AC16O9_10880"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(21263..21910)
                     /locus_tag="AC16O9_10885"
     CDS             complement(21263..21910)
                     /locus_tag="AC16O9_10885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6517160.1"
                     /translation="MASRAVLFDLDGTLADTAPDLAAALNRLRTDQGLEPVPLSRLRP
                     FASAGARGLVHAGFGVKPGDDDYNALREAFLEAYRENTCVHTQLFPGIAELLAELGRR
                     SLAWGIVTNKATRFTDRIVETLGLKPACVVCGDTTEHLKPHPAPMRHAAEQLKLPAGE
                     CMYLGDDLRDIQAARAADMRAIAVGWGYHHPENGGPRSWKADAVITQPADLIALL"
     gene            complement(21903..22613)
                     /gene="ubiG"
                     /locus_tag="AC16O9_10890"
     CDS             complement(21903..22613)
                     /gene="ubiG"
                     /locus_tag="AC16O9_10890"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR6517161.1"
                     /translation="MQTAAPNVDPAELEKFSALAHRWWDPHGEFRPLHAINPLRLEWI
                     EHHAPLAGKDVLDVGCGGGILAEAMAQRGARVTGIDLAEKPLSVARLHLMESGLEVRY
                     ELASAEDYAAAHAGAFDVVTCMEMLEHVPEPASTVAACARLLRPGGCAVFATINRNPK
                     SYLFAIIGAEYLLRLLPKGTHDYARFIRPSELAHWSRDAGLREVALSGMTYNPFTQRY
                     RLGPDCDVNYLLCCRRDG"
     gene            complement(22731..22883)
                     /locus_tag="AC16O9_10895"
     CDS             complement(22731..22883)
                     /locus_tag="AC16O9_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517162.1"
                     /translation="MEQFLSSLQPTLGQHIPGILGAIAVALSFGLGGREAADKQMEYW
                     LAKWRK"
     gene            complement(23232..23885)
                     /locus_tag="AC16O9_10900"
     CDS             complement(23232..23885)
                     /locus_tag="AC16O9_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6517163.1"
                     /translation="MTGLYLWVRISNPDNPLGNWRKRMPKNIFRSAGLSAALVAMAVL
                     VGCATTEEPAPPPPAPAPQPAQPAPAPAPAPAPAPAPAPAPAPKPAPKPVAQKVTLAA
                     DVLFDFDKSVIKPDYKDRLDNLSTQIRDINLEVVIAIGHADLIGTLQYNQKLSVRRAE
                     AAKAYLVSKGIEPNRIYTEGKGETQPVKACPKLSGKALIECLQPNRRVELEVIGTKK"
     gene            complement(23882..25201)
                     /locus_tag="AC16O9_10905"
     CDS             complement(23882..25201)
                     /locus_tag="AC16O9_10905"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR6517164.1"
                     /translation="MTERLLINARWVVPVEPAGTVLERHAVAVRDGRIEALLPAAQAA
                     ERYPDYARIELPGHALIPGLVNAHTHAAMALMRGMADDLPLMRWLEEHVWPVEAKHVS
                     AEFVKDGTLLACAEMLRGGVTCFNDMYYFPEAALEAALQAGMRAFLGLIVIEFPSAYA
                     SDPQSYLAQGLALRDRWREHPLAAFCLAPHAPYTVSDATFRRVAQLAAELELPVHVHV
                     HETAGEVERSMAEHGMRPLERLAGLGLLGPGLIAVHAVHLSPPEIALLSRHRASVAHC
                     PSSNLKLASGLAPVAQLLAADVNIALGTDGAASNNRLDMFEEMRLAALLAKAAGDDAE
                     AMPAHAALHAATLGGAIALGAEARFGSIVPGKAADLAAVALDDVALQPVYDVVSHLVY
                     ACGREHVTDVWVGGERVLRDRRLTRIDAADLQGRAALWQTKILNSLS"
     gene            complement(25264..25857)
                     /locus_tag="AC16O9_10910"
     CDS             complement(25264..25857)
                     /locus_tag="AC16O9_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6517165.1"
                     /translation="MNRRTAQLFVCATALAAMVGCATTEEAPVAPAPAPAPATPAPAP
                     AAPAPAPAPAPAPAPKPVAEPAKPKPLRVFSHVLFAFDKSVLSMGAQDTLDREVVVRF
                     GEFSDVRFININGYTDRIGSVQYNQKLSERRAEAVKAYLVRQGAEGSKIQTTGYGKMY
                     VGSTLPVVSCDQKDRKALIACLQPNRRAEVEVLGTGR"
     gene            25994..28621
                     /gene="gyrA"
                     /locus_tag="AC16O9_10915"
     CDS             25994..28621
                     /gene="gyrA"
                     /locus_tag="AC16O9_10915"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6517166.1"
                     /translation="MEPFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMQESNNVWNRPYVKCARVVGEVLGKYHPHGDSATYEALVRMAQDFSMRYML
                     IDGQGNFGSIDGDNAAAYRYTECRLARLSAEMLADIDKETVDFVPNYDGKELEPAVLP
                     TRVPNLLVNGSSGIAVGMATNVPPHNLGEVADACLHLLENPHCAIEDLMKLMPAPDFP
                     TAGIIYGLSGVHEGYRTGRGRVVMRARTHFEDMEKGNRQAIVVDELPYQVNKKALLEK
                     IAELVTEKRIEGISDIRDESDKDGIRVVIELRRGELPEVVLNNLYKQTQLQDSFGMNL
                     VALVDSQPKLLNLQQLLEAFVSHRREVVTRRTVFDLRKARERGHVLEGLAVALSNVDE
                     VIALIKAAPAPADAKRELMARAWDSALVREMLSRGSAQAYRPEGLAAEFGLREDGYRL
                     SDAQAQAILELRLQRLTGLEQDKIRAEYKEVMEQIADLLDILDKPARITAIIGAELKK
                     LKEDFADPRRTEIVTVAEDISIEDLIAPQDMVVTFSHGGYVKAQPLADYRAQRRGGRG
                     RAATAMKEDDFIERLFVAHSHDYLLCFSSRGILYWLKVFEVPQGSRASRGKPIVNVFP
                     LQEGEKITAVVPVKEFDEQHFVLMATARGTVKKTPLAEFSRPRQAGIIAVSLEDGDYL
                     VGGVLTDGSADVMLFSSEGKAVRFAESEVRPMGRQAAGVRGMRLPEDGSQRVVCMLVA
                     RDESLSVFTATEHGYGKRTPISEYPKHGRGGQGVIAIQASERNGRLVGAVLVDDSDEV
                     MLISTRGVLIRTTVAQVRAQGRSTQGVTLIALGEGEKLAGLERIEEKDEDVPGNGNGN
                     GDGGEAPLPPAEELPPTVH"
     gene            28621..29697
                     /gene="serC"
                     /locus_tag="AC16O9_10920"
     CDS             28621..29697
                     /gene="serC"
                     /locus_tag="AC16O9_10920"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR6517167.1"
                     /translation="MSRLFNFSAGPAMLPAEVLARAGDEMLDWRGSGMGVMEMSHRGK
                     EFMAIAAEAEADLRELVAIPAHYKVLFLQGGATLQFAQVPMNLLRGRSGADYVVTGEW
                     SKKAVKEAKAYCDVHVAASSEDRKFAYAPRTWNVRQDAAYVHYCSNETIGGVEFHWVP
                     ETGAVPLVADASSHFLSRPLAVEKFGLIYAGAQKNAGPAGLTIVIVREDLIGHAAKGT
                     PTVMDYKLQADAGSMLNTPATYSIYIAGLVFKWLKQLGGLAEIERRNVAKARLLYEVI
                     DGGSFYGNPVDKADRSRMNVTFRLPDERLDAPFLKGAEERGLSQLKGHRAVGGMRASI
                     YNAMPPEGVEQLAGYMREFAARHG"
     gene            29690..30883
                     /locus_tag="AC16O9_10925"
     CDS             29690..30883
                     /locus_tag="AC16O9_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR6517168.1"
                     /translation="MAERAGKLEVLVLNRVAQAGLQRFPAERYALVTEASAPHVVLVR
                     SQDLHRAALGERLQAVGRAGAGTNNIPVGALSKRGVPVFNTPGGNANAVKELVIAGLL
                     MAARNLAPALDFVRGLDKAAPDFERRIEDGKKAFAGGELPGQTLGVVGLGKIGSLVAD
                     AAIRLGMQVIGYDPDITVEGAWSLPSQVRRAASVDELMRASRFVTLHVPLAERTRHIV
                     NAKNVALMREGAILLNFSREGVVDDAAVLAALAAGRLRGYVTDFPTPALVGAPGVVAL
                     PHLGASTQEAEENSAVMVVDQVREYLEHGNLRNSVNFPEAVMARESPYRLAIANANVP
                     DMLARISHAMGERGINIHNMLNKSRGDVAYTLVDVDSPVPEAALSALRAIDGVLAVRY
                     LPLGP"
     gene            30880..31956
                     /gene="pheA"
                     /locus_tag="AC16O9_10930"
     CDS             30880..31956
                     /gene="pheA"
                     /locus_tag="AC16O9_10930"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR6517169.1"
                     /translation="MSGDIDKLRTEIDALDAELVALLQRRARLAQRIGAAKGGAAAYR
                     PEREAEILRRVAAQGGEPLPAAALTRVFREVISACRGLEQEIRVAYLGPPGTFSEQAV
                     RRHFGGSVEGVPAASIDEVFRSCESGAAQFAMAPAENSTEGVVGRTLDLLLVTPLRIC
                     GEVELRVQQNLMAQHADSSRLKRVYSHAQSLAQCSAWLNHHLPGAERVPVASNAEAAE
                     RAAREPHAGAIAGEAAAERYGLQLLARAIEDDPNNTTRFLVLGTLDPAPTGRDRTSLV
                     MSAENKPGAVHALLTPLAENGVSMTRIESRPARAMRSALWEYVFFIDLEGHASEARVA
                     AALEALRRKAPFLKVLGSYPAAGT"
     gene            31970..33079
                     /gene="hisC"
                     /locus_tag="AC16O9_10935"
     CDS             31970..33079
                     /gene="hisC"
                     /locus_tag="AC16O9_10935"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6517170.1"
                     /translation="MIMDPCESSPSYVRSIAPYQPGKPISELARELGLKEETIVKLAS
                     NENPRGIGPRTRAAIERALGEVARYPDGNGHALKTALAARYGVDMAQIVLGNGSNDVL
                     ELVALAFLGPGRAAVYSQHAFAVYPLATQARGARGIVVPARDYAHDLEAMARAVDDDT
                     CVLWVANPNNPTGTYATGDALEALVRRVPERVIVVIDEAYNEYLPADLRVDTVKWLKR
                     HSNLVITRTFSKAYGLAGLRVGYALAHPSVADVMNRVRQPFNVNSIALVAAAAALEDM
                     EFVAKSFAGNLQGMRQLRDGAQQLGLAFIPSYANFLTVRVGKAQDIYKRLLRRGVIVR
                     PVGGGYELPEHLRVTVGTEEENERFLSALAASLKD"
     gene            33079..33939
                     /locus_tag="AC16O9_10940"
     CDS             33079..33939
                     /locus_tag="AC16O9_10940"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR6517171.1"
                     /translation="MHFAKVAVVGVGLIGGSFALALKAARACGDVVGVGRGAANLKLA
                     LEQGVIDAVGADIADADLVLVATPVAQYPRVLAALAPRLKASTIVTDAGSTKRDVIAA
                     ARAALGAKIAQFVPAHPIAGAERSGASAASAELFRNRRVVLTPLPENPAAAVQAVEAA
                     WQACGARVSRMEAAEHDAVLAAVSHLPHLLAYALVHEVAGRADSAQLFGFAAGGFRDF
                     TRIASSHPEMWRDICVANRDRLLDELDRYLAKLQAVRPLVENGDGAALEKLFAEARAA
                     RQRWLAGEYE"
     gene            33936..>34744
                     /locus_tag="AC16O9_10945"
     CDS             33936..>34744
                     /locus_tag="AC16O9_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009921655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6517172.1"
                     /translation="MKTLDLGPLSRAAGTVRLPGSKSISNRVLLLAALARGDTEVRGL
                     LDADDTQVMRAALGRLGPQLSVKQAELDLGNAGTAFRPLTAVLALSGGEYRLSGTRRM
                     HERPIGDLVDALRALGARIDYLGAEGFPPLAIHPGKVASGQTVRVRGDVSSQYLSALL
                     MALPLAGGGTIEVAGELISKPYVEITLNLMRRFGVDVTRDAWRRFIVPGTRYRSPGSV
                     QVEGDASSASYFLAAGAIGGGPVRVEGVGRDSIQGDVAFTGVLERMGAAVTL"
BASE COUNT         5430 a        11756 c        12172 g         5386 t
ORIGIN      
        1 tttcggtgcc gtccttcatt cgaaccttga tccgcccgga gacagcgtag ccaacgtgag
       61 acgcctggca ctggtcggtc ttaacgacgg gtttgacgca ttctgaccag cgccagccgg
      121 gctcgaagtt gaaacgcccg atcgtgaacc cctcgagttg cacgacctcc acccgggtct
      181 tgttcgggga gcgaacctca tcgggggagt tgtgtgactt tacttggaac gtggttactg
      241 aaactggagt tgccatatct ttctctcctc tctagatggg ccgcggccgg caggcgcggc
      301 tacgtcgccg agtcaaagca cgttactttg gcagtcagaa acggactgaa gtgacggcat
      361 cccagctctt cgatcttatg cgctaatcaa cccgtcatgg cggctaacgt tcggcgtcag
      421 gcgcgcccgc ttgcgggcgt cggctgcacg ccgtggttag gctcacgctc gtgcacatct
      481 ggcttgaatg ccatcgcagc ggcgacgtca aaacttggga atgccgtcct caagcgtgaa
      541 attcaacgta gcgccgctcg tcacgtttat caccacaagc tcattgggct tcttacggtt
      601 gcgctggaac cgaaagcagt ttgcaggccc gtcacttggc gcgacacaca cccaatcgtt
      661 gcctttgact tcccaggtgc gcggctgtgg tttgccgccc tgcgagatga agattccctt
      721 gccatcggcg cggtagtgtc cgacccatcc gttgccacgg aaggtcttgt tcgagtaaag
      781 agcgcgcaac tcgatcgggt ccttaatgtc gacccagtca cttgagctct gcgccaacac
      841 tggcgcagga agcacgaaaa ccatcgtggc catcagaatc gcgagatttc ctgatctcat
      901 ggtgttgccc tcctgcgagt tagtggagaa cggacaggac cgtcaagtct cgacaatatc
      961 gcgcgtcatg cgttacccat cccgtgccgc ccacgtgagc ctaacgtcgc cttcagcggc
     1021 gcgctgttct tggcgcgtcc gctgcaagag cctgttggac cgcgacctgc aaagagcgca
     1081 cgcgacttct cccggtcact gaagcacgac ggcggtcttg ataacggaag atcttgagct
     1141 catcgcatcc caccaactgg cgagccgggg cagtccggct gcgaccgcct ttccctctgg
     1201 tgtctgaaca aagtacgccc acatgggcac gaggtacaga tcggcaagcg agatcgaggt
     1261 ttcgatcagg aatgaggggg cctccacgag accctcgaga actgccaacc ccttgcgaag
     1321 tcgacgcagg ccagagtcaa cgatgctttg gtccggcggc tgcccaagca ccggctttat
     1381 cagtcgttcg accaagatct gcccgaccat cgcggggtag atgtaagaga caatgacccc
     1441 gatcgcttgg agcatccggg cgcgaacgcg tgcgtccttt ggttgaagcg ttgcgcccgc
     1501 gaatgcgctg tcgacgtagt cgaggatcgc agcggtctcg aagagcttca aacgcccatg
     1561 ctcgaggacc gggactttgc cgaagggatg acgtaacagg tgttcggggc ttttgttggc
     1621 tccacgcaag aaatccactt ctacgagcgt atagccgacc cccttttctt ccaaagcgag
     1681 tcgggccgtt cgggtgtatg tgctgtacgc cgggccgtac aagacgatct cggctgcagt
     1741 catggcgttc acgcggtcca acgttcaata accagcataa agtgcggcat atcatagacg
     1801 cgttcttcca gtagcgctgt caacccttcc tgcaatcaac gcgttacgga tatgtatgca
     1861 tatccggtac ccgtgatagg ccgcaggaaa tggtacaaag ctctgaacta ccggaggtat
     1921 cttccggctc gatagctcaa ccccggctgc gcgaccgctt ccgcggcgag attcgcaagc
     1981 gcggctatag ccgcaggacc gagaagtctt attggttctg gatccgctgg ttcatcttcc
     2041 acaacgggac gcggcatccc gccgagctcg gcgcgcgcga ggtcgaggcc tttctgagct
     2101 ggctcgcggc ggaacgcaac gtcgcggccg ccacgcagaa tcaggcgctc gcggcgctgc
     2161 tgtttctgta caagcaggtg ctcggacagg acctcccgtg gttctccagc atggtgcggg
     2221 cgaagcggcc ggtgcgcgcc ccggtggtgc tgacgcaggc cgaggcggcg cggcttctcg
     2281 agcagctgca gggcgtgaaa tggctgatgg cgagcctgct gtatggcgcg gggttgcggc
     2341 agatcgagtg cctctcgctg cgggtgaagg acctggactt cgcctaccgg cagatcacgg
     2401 tgcgcgacgg caagggcggc aaggatcggg tgacgatgct gcccgagcag gtggtgcagc
     2461 cgctgcaggc gcacctgggc cgggtgcgcg cgctgcaccg gcgcgatctc gctgccgggc
     2521 acggcgaggc atcgctgccc aaggcgctgg cgcgaaagta tccgcgggcg ggtcgcgagt
     2581 gggcgtggca gttcgtcttt ccgtcaagga atccgtccgc ggatcccgag tcgggcgtca
     2641 tacgccggca tcacctgttt ccggacacgc tgcagcgcgc ggtgaaggag gcggcgaaag
     2701 cggcgcgcat cgtgaagccg gtgagctgcc acacgctgcg gcactccttt gcgacgcacc
     2761 tgctcgaggc cgggcacgac atccgcacgg tgcaggagcc gctcgggcac gcggacgtgt
     2821 cgacgacgat gatctacacg cacgtgctca acaagggcgg gcgcggggtg aagagcccgc
     2881 tggatcggct ggagcagccg ggcacggcgt acgagccgcg aaccacaggt tgaggtgatc
     2941 acattctgtg atcacccacc gcagaagaaa ctgaatatca gccagttacg aacttgaggt
     3001 cgcaaattgc gacctcaaga ttggagatgg caggggggtg caaacagcag cccccttggc
     3061 ccgctcaact gaacgcctgt attcccgtga tcgcccgccc caggatcagc gcgtggatgt
     3121 cgtgcgtgcc ctcgtaggtg ttcaccactt ccaggttcac cacgtggcgg atgacgccga
     3181 actcatccga gatgccgttg ccgccgagca tgtcgcgcgc ggtgcgcgcg atgtcgagcg
     3241 ccttgccgca ggagttcctc ttcaggatcg aggtgatctc gggcgaggcg gtgccctcgt
     3301 ccttcatgcg ccccaggcgc aggcacgcct gcagcccgag cgcgatctcg gtctgcatgt
     3361 cggcgagctt cttctggatc agctggttgg cggcgagcgg ccggttgaac tgcttgcggt
     3421 cgagcacgta ctggcgcgcg cggtggaagc agtcctcggc cgcgccgagc gcgccccagg
     3481 cgatgccgta gcgcgcggag ttgaggcagg tgaaggggcc cttcaggccc cgcacctcgg
     3541 ggaaggcgtt ctcctcgggc acgaagacgt cgtccatcac gatctcgccg gtgacgctgg
     3601 ttctgaggcc gaccttgccg tggatcttgg gggcgctgag gcccttcgcg cccttgtcga
     3661 ggatgaagcc gcggatctcg ccgacgttgc ccttctcgtc gacttccttg gcccagacga
     3721 cgaacacgtc ggcgacgggc gaattcgaga tccacatctt cgagccggtg aggctgtagc
     3781 cgcccttcac cttcttcgcg cggctcgcca tcgagccggg atcggagccg tggttgggct
     3841 cggtcaggcc gaagcagccg acgtactcgc cggtggcgag cctggggagg tatttcttct
     3901 tctgcgcctc ggtgccgaac tcgaagatcg gcagcatcac gagcgagctc tgcacgctca
     3961 tcgtcgagcg gtagcccgag tcgatgcgtt ccacttccct cgcgatgagg ccgtagcaga
     4021 cgtaggaaag gcccggcccg ccgtactcgc tcgggatggt ggggccgaga aggcccagct
     4081 cgcccatctc gcggaacacc ttgaggtcgg ccttttcctc gcgaaaggcc tcgagcacgc
     4141 gcggcgcgag cttgtccctg caataggcgg ccgcggcgtc gcgcaccatg cgctcgtctt
     4201 cggtgagctg cgcatccagc agcagcggat cttcccagct gaaccgggcc ttgggcttgc
     4261 tcttgagggg ggcgttcata agctctttcc taaagttcgt gctgctgcgc aggtcgcatc
     4321 tgcgctgcag cacatcgtta cgttctttgg aggatcaccg cgaggccctg gccgacgccg
     4381 atgcaaagcg acacggcggc gagcttgccg cccgattgct gcagctctct tgccacggtg
     4441 agcgcgaggc gcgcgcccga gcacccgagc gggtgcccga tggcgatggc gccgccgttc
     4501 gggttcacgc gcgggtcctt gttgtccagc ttcatcaggt tgaggcaggc gagcacctgg
     4561 ctggcgaagg cctcgttgat ctcgatgatg tccatttcct tgagcgattt gcccgcgcgc
     4621 tcgagtgccc gcgggatggc gtaggcgggg cccacgccca tgatccgtgg ttccacggca
     4681 gcactcgccg ccgaaagtat tctggcgatc ggtttcttcc cattccgatc gccccacttg
     4741 cggctggcga tgatgagcgc cgcggcgccg tcgttgacgc ccgaggcgtt gcccgcggtg
     4801 accacgccgc cttcgtacag gggcttcaac ttggcgagcg actccatggt gctgtcgcgg
     4861 cgcgggtgct cgtcggcctc gaccttcacg ggcggcgcgt ccttcttctt ctgcggcagg
     4921 gcgatcgcca tgatctcgcc cttgtagaag ccttcgctct ccgccttggc gtacttcttc
     4981 tggctggcga gcgcgaactc gtcggcctgc tcgcgcgtga cgccgaactc cttggccacg
     5041 ttgtcgccgg tctcgggcat ctggtggtcg ccgaagagct tcttgaatct cgggttggtg
     5101 aagcggctgc cgatggtgct gtcgtagagc ttcacgtcgc gcccgtacgg cgtctcgctc
     5161 ttggcgaaca cgtacggcgc gcgcgacatg ctctccacgc cgcccgcgat gtagagctcg
     5221 ccttcgcccg cggtgatggc gcgcgcggcg tcggtcaccg cctgcaggcc cgaggcgcac
     5281 aggcggttca ccgtcacggc cggcgtggtc ggcggcaggc ccgccatcag cgcggcgaag
     5341 cgcgcgacgt tgcggcagtc ttcgcccgcc tgcgtcacgc agccgaggat cacgtcggag
     5401 atgtcctcgg gcttgacgcc gtttcgcttc accacctcgg cgatcacttc gcccgccagg
     5461 tcgtcggggc gcatcggcgc gagctttccc gcgtggcggc cgatggggga gcggattccg
     5521 tcgtagatat aggcatcgag catggggtta ctccggggtg agcagcgaca cgcccaggcg
     5581 cgcgcggcgc gtgagccagg ggctggggcg gtagcggggt tctttggtga actcgtgcat
     5641 ggcttcgagg atggcgagga ccttcgccgg gccgaccgcg tcgcccaggc ccagggggcc
     5701 cttgggatag cctaacccga gcatcaccgc gcggtcgagg tcttcgggcg tggcgatgcg
     5761 ctgctgcacg atatcgcagc cgacgttgac gatgtgcgcg acgacgcgct gcgcgacgaa
     5821 gccggccgaa tcgcggatca cggacaccgg cacgccgtcg gcggcgagaa gcccgtgcgc
     5881 ggcttcgcgt gcttccctcg tcgtcaccgg cgtgggcatc agcgtgcggc gcttcgcgaa
     5941 cccgaacagg ggatcgatgg ccacggtgtg cttcgggtcg aggcccgcct ggagcgccgc
     6001 cgtggtcgca tccgcgccga gcggggcgac gaggcacagc gttcccgcga gcggcttggc
     6061 ctcgagcttc gcgccgaggc tggcggcgaa gtccttcagc tccggtgccg cccagaccga
     6121 tgccgccttc gcctgcggca ccggctgctc ggggatcttc tcgggggcgt tgtccttgcc
     6181 gtagcggtac cagccgcgcc cggtcttcct gcccagaagg cccgccgcga cgcgcgtttc
     6241 ggcgatgtag gccggctggt acttcggctc gccgtggtat tgctggtgca tcgacttcat
     6301 caccgcgtgc gcgacatcca gcccgacgag gtccatcagg ctgaacggcc ccatgcgaaa
     6361 gcccgccgcg tcgacgagga tgcggtcgat ggtggcgaaa tccgcgacgc cttcctgcag
     6421 cacgcgcagc gactcgggca cgaagccccg gcccgcgtgg ttgacgacga agcccggcgt
     6481 gtccttgcag cgcaccgcgg tgtggccgaa gcgcttggcg aggcccgcga gcgcctcgcc
     6541 gacccagggc tcggtcagct cgccgtccac cacttccacg atcttcatca gcggcaccgg
     6601 attgaagaag tggtagcccg cgacgcgccc cggcctggcg caggccgcgg ccatcgcggt
     6661 caccgagagc gaggaggtgt tcgaggcgag gatgcaggta tcgccgacga tcgcctccag
     6721 ctcgcgcaag agcttctgct tgaccgccag atcctcgacg atcgcttcga tcaccagatg
     6781 gcagtccttg aacgcggcgg gggcctccgc gatctcgatg cgcgcgagcg cggcgtcggc
     6841 gtcggcctgg gcgaggcggc ccttggccac caccccggcg agggccttgg cgatggcgtc
     6901 cttcgccttg gcggcggcgc ccgcctgcgc gtcgaagacg cgcacgcgcg cgccgcactg
     6961 ggcgagcact tgaacgatgc cgcggcccat ggtgccgctg ccggccacgc cggcgaggag
     7021 gtcgctgcgg gaaggatcga gcatgggggt caaaggccga ttctatccgc gcgcgggcca
     7081 aggctataat cccccgcaca aaacaggcgc ggaggacggc catgaaatac cgcaagatcc
     7141 tgctcgcgta caacggttcg gcggagggga aacgcgcgct gctcgagtgc gcggagctgg
     7201 cgaacttcct gggggcggaa acgcacctcc tggcggtggc gagcatgccg ccgagcctgt
     7261 ttctcaccga agggttcgtg cccgaagagc tgctggagga ggaaaagaag cgcacgcaga
     7321 cggtgctcga cgagggcatc gccaacctga aggaccaggg cttccaggcc accgggcacc
     7381 tcgcggtggg cgagccggtg gaggagatct gccgcctcgc gaaggagctc gcggtggacc
     7441 tgatcgtcgt cggccacaac cagaacacgt cgttcgcggc gcgctggtgg aagggctcgg
     7501 tcggcgcctc gatcctcgac tacgcgccct gcagcctgct ggtcgcgctg tcgcgcaact
     7561 agcggaggtt ttctcgatgg agagtttcgg acaccagggg cgcctggagg atgagcgcat
     7621 gctcaagggc gccgggcgct acgtctcgga ctggacgctg cccggggagg cgcgcggttg
     7681 tttcgtgcgc tcggaccggc cgcacgccga gatcgccggc atcgacgcga aggccgcgct
     7741 cgccatgccc ggcgtgctcg cggtgatcac gggcgcggag ctcgtccagg caggcctgaa
     7801 gccaattccg gccgcggcgc ccttcaagtg gagcgacggc tcggagcagc gccaggcgct
     7861 gcgcctgtcg ctcgcgcacg gcaaggtgcg gcacgtcggc gagccggtgg cgctggtggt
     7921 cgcccagacc gcggcgcagg cgcaggacgc ggccgaaagc gtcgtcgtgg actacaagga
     7981 cctgccggcg gcggtgacgg cggaagaggc gctcgccaag ggcgcgccgc aattgcacga
     8041 gatcgcgccc ggcaacctcg cgctcgattt caccggcggc gacgaggccg ccgccaaggc
     8101 cgcgttcgaa aaagcggcgc gcgtggtgaa gctgaaggcc taccacacgc gcgtggtggg
     8161 caacccgatg gagccgcgcg cggcgatggg ctcgtacgac cccaagaccg agacctactt
     8221 tctgcacgcg accacccagg gcgtggggcc gatgcgcgcg cagtgctcgg cggtgctcgg
     8281 cgtgccgccg gaaaaggtgc gcgtggtggc cgaggaggtg ggcggcggct tcggcgtgcg
     8341 cttcaacgcc tatcccgagt acggcgcgct cctctacgcg gcgaagaagc tcggccggcc
     8401 ggtgaagtgg gtcggcacgc gctcggaggt gttcctcggc gacgagcagg cgcgcgacat
     8461 cgtgcacgtc gcggagatgg cgctggatgc gcaggggcgc atcctcggca tgcgcttcga
     8521 ctacctggcg aacctcggcg cctacattgc cttcaccggc gcggtggtga acaccatcgg
     8581 catggtgagc gtgatctgcg gcgtctacga cgtgcaggcg atccacgtgc gcgggcggct
     8641 ggtgctcacc aacaccgtgc cgaccgcggc gtaccgcggg gcggggcggc cggtcgcgtc
     8701 ctacgcgctc gagcgcctgg tcgaccaggc ggcgcacgag ctgaagatgg acccggcgga
     8761 gttccggcgc cgcaacatga tcccgaagtc gaagatgccc tacaagcagg tgagcggctt
     8821 cgagtacgac tgcggcgatt ttcccggtgt gctggagcag gcgaagaagg aagccgactg
     8881 ggacgggttc gacaagcgcc gcgccgagtc gaaaaagcga gggaagctgc gcgggcgcgg
     8941 catctccgcg tacatcgaaa tgaccgcgcc cgggggcttc gcgccctacg accaggtggt
     9001 ggtcagctgg gacaaggacg gcggcgtgac gctgcgcacc gcctcgcaca accacgggca
     9061 ggggcacgaa acggtcttcg cgcagattac cgcgcgcgtg ctcggcatcc cgatgcacaa
     9121 gatccggctg aagacctccg agcccgacac gaacctggtg gcgaacccga ccggcggctc
     9181 gcgcacggcg cacggcctgg ggagcgcggt gctcctcgcc gcgcgcgaga tcgtggacaa
     9241 gggcatggac ctggcggccg acgagctcga agccgcgaag cccgacctcg agttcaagga
     9301 aggcgagtac cgcatcaagg gcaccgaccg gaagatcgcc atcaccgcgc tggcgcaaaa
     9361 gcacccgggc aagctcgacc tcgacttcaa ggagcgcccc aaggtccctt cgacctaccc
     9421 gaacggttgc cacatcgcgg aagtcgagat cgagcccgag acgggcgagg tggagatcgt
     9481 gtcctacctc gcctgcgacg acgcgggcaa cctcctcaac gagcagctcg tgcacggcca
     9541 gatgcacggc ggcatcacgc agggcgccgg ccacatcttc ggcgagcagg cgatctacga
     9601 cgactcgggc cagctcctca cgggaagctt catggactac gccatgccgc gaccgggcct
     9661 ggtcggcggg ctgcgcgtca ccgagcatcc ggtgcccacg gcgaccaatc cgctcggcgc
     9721 caagggcgtg ggcgaagccg gcgtcaccgg ctcgatgccg tgcctgatga acgcggtgat
     9781 ggacgcgctg cgccaggaag gcgtcacgca cttcgacatg cccgcgagcc cgctgcgcgt
     9841 ctggcgcgcg atccgcgaag ccaaagccgg caaccccgcc gccctggccg taaaacaata
     9901 aatggggaca gacctcattt gtttttggcg ccggtgacga aactcacgtc gccccgggtt
     9961 tcttgccctt caatacatct cggcttttct ggccggaaga ttcacccggc cagaaaagcc
    10021 gagaccatag agaaccgaac gtaggcctcg gcgcactcgc gtcgccggcg ccaagaaaaa
    10081 atgaggtctg tccctattta aacttgcgct cttcccacca ggggtagaac ggcggcatgt
    10141 cgcgcgagac gcgcatgccg aaatcgggcg cgcgcttttc cttgaatgcg gtgatgccct
    10201 cggcgacgtc gggcgaggcg cccatgtaga agatgccgcg cgagtccacc ttgtgcgctt
    10261 ccatcgggtg atctgccccg agcatgcgcc acatcagttg ccgcgcgagc gccaccgaca
    10321 ccggcgcggt gtgctcgacg atctccctgg cgagtgcgcg cgcagcaggc aggagctcct
    10381 ccgggggcac gactttcgac accagctttc ccgcgagcgc ttcctgggcc gggaacacgc
    10441 gccccgagag cgtccattcg agcgcctgcg agattcccac caggcgcggc aggaaccagc
    10501 tggagcaggc ctcgggcacg atgccgcggc gggtgaagac gaagccgaag cgcgcctcgc
    10561 tggaggcgag gcgcacgtcc atcggcagga gcatggtggc gccgatgccc accgccggcc
    10621 cgttcaccgc ggcgatgacg ggcttcttca tctcgaagat gcgcagcgtc acgcgcccgc
    10681 cgccgtcgcg gtgcgtgtcg gcggaaaggc cctcgcgcct cgcgtaatcg aaggtctcgc
    10741 gcccggccga gaggtcggcg ccggcgcaga acgcgcgtcc cgcgccggtg acgatcaccg
    10801 cgcgcacctc gtcgtcggcg tcggccttgg agaacgcgtc gaccagctcg tcgcgcatca
    10861 cgtgggtgaa ggcgttcagc ttgtcggggc ggttgagcgt gagggtggcg acgcgctcgg
    10921 cgaggtcgta gcggatctgc tggtaggcca taggtaaatg aggtctgtcc ctaatttatg
    10981 cgccgctcgc ggccgcgcca gtagggctcg cgcagcacgc gcttcaggat cttgccggtg
    11041 gcgaacgcgg tgttcgccgc ctccgggcag cgcatccgca agctgccgat ccggctcgcc
    11101 tagcgcttgc cgacgacgcg ctcgaccatc tcgcgcgcgg cgccgaggta gttcgacggg
    11161 tcggtgagct tctcgatcgc ctggcggtcg aggtgccggg tcacctccgg caccctggcg
    11221 agcgcctcgg cgaggctgcc gcctttctcg gccaccgcac ggcaggcgtc gtagaccacg
    11281 tcgtgcgctt cgtttcggcc caagtgcggc gcgaggccca tcatcacggc ctccgcgacg
    11341 atgaggccgt gcgtcatgtc gaggttcttg cgcatgcgcg cctcgtcgac gatgaggccc
    11401 gccagcatga acttcgcctg gtgcagcgcg ccgcccgaga gcatgaacgc ttcgggcagc
    11461 gcgatccatt cggcgtgcca gggtccggtg gcacgctcga agtcctgcac cagcgcatcg
    11521 agcatcaggc ctgcgtgctg gcgcaccgcc ttcgccgcgg cgaggatcag ctcggaggaa
    11581 atggggttgc gcttttgcgg catggtgctc gaggcgccgc gccccggcac gaacggctcg
    11641 ctcacttccg cgacctcggt cgaggccatc agcatcacgt cgaaggcgac cttgcccagg
    11701 gacccggtga ccaggccgag gaagttgacg gcctcggcaa ggccgtcccg cgccgcgtgc
    11761 caggtggcga ccggctgtgc gagcttgagt tcctccatga ccgccttctg caccgccatg
    11821 cccttgtcgc cgagcgacgc gagcgtgccc gcagcgccgg cgaactggcc gacgagcacg
    11881 cgcggcttca actgctgcag ccgctcggcg tgccggtcga ccatcgcgag ccagatggcg
    11941 agcttgtagc cgaaggtgac cggcagcgcc tgctgcaggt gcgtgcgccc cgccatcggc
    12001 gtgtcgcggt agcgctttgc ttgcctggcg aggatcgact gcagctcggc gaggtccttg
    12061 tcgatcagcg ccagcgcgtc gcgcacctgg agcaccaccg ccgtgtccat gatgtcctgc
    12121 gtggtcgcgc cccagtggag gtagcgtccc gcctcgccgc actgcgcggc gagctggcgc
    12181 accagcggca ggatcgggta gccgaccgtc tcggtctcct gcttcagccg ctcgagatcg
    12241 agcttcgatg cgtcgcactt cgcggcgatg tccttcgccg cctgcgccgg gatgacgccg
    12301 agcctgcctt gcgcgcgcgc gagcgccact tcgacttcgg tgtagcgagc gaggagggcc
    12361 gcgtcctcga agatggcgcg catcgccggc gtgccgaacg agtcgcggaa cagggccgag
    12421 tccagaaccg tcgatgccat gggcgtctcg tctaggaggg agtgtctggc gcctgcatcc
    12481 acgcggggac gtcgcgctgc ccgtgaagca cgcgccagac gtcgacccgg tcgtccaggc
    12541 cgagatggaa aatgagaaac gggtagccag caagcgacca gcagcgtagc ccgggaaggt
    12601 tcagttcgag cgcgtagcgc ggcgagccgc tctgcggaaa gcgagcgagg tgcgcatacg
    12661 ccttctcgag cgccgcgatg aatcccatcg cggcctgcgg cgcgccctgg tccaggtagt
    12721 acgcgatcgc ctcgtccacg tcccggtttg ccagctcgcg cgggacgacc ggcttcgatc
    12781 tcacctacgg ttgccggatc gtccgcgtcc gcgcacgcgg cgccggagcc gggcgaagta
    12841 ggccgcatcg gccgggtccg cgagcctcga ttccgctccc gccacgagca ggccgcgcag
    12901 cgtctggcgc tcctggtcct tgcggatcag ctcgcgaacg tactcgctgc tggtgccgta
    12961 gccgcggctg ttcacctgct cgtcgacgaa atccttcagc gcgtcgggca gggagatgtt
    13021 catcgtgctc atgcggcaag cttaggcggc ttggcaaagt ttggcaagcc acgcggcgag
    13081 cggctgaccg ggccgcgcgc cgagccccga cgccggcgcg ttcgcgaacg agatcgtccc
    13141 gctctcccac atcgagcgcg cttcgccgat gcgcgcgctc atcgtcgcca cggtcaccgc
    13201 cgggatgccg cgtgccgcga gcaccggcag ccgcgtcatg tcggcttgcc tcgcgccggc
    13261 atcgtggaac gccgccgcgc gcgccggcac cgggatcgcg gtctccacgc gcccgccgtg
    13321 cagcgcgccg tgcgagccga agatcaatac cttcccggcg tcctgcggcc gcgcgagccc
    13381 caccgagtcg agggcccaga ttccggcgtg cacttcgaaa cggccctcgc cgggcgaggc
    13441 gagcgtgccg cgcggcgccg gcgcctgcgt caggcgcacc gcggcgtcgc gcaccggcat
    13501 gcccggttcc acgccgcagg cggcggcgag cctgttcgcg cggctgatga cgccgcgcgc
    13561 gagcgtatcg gtgccgtcgc cgatgcgcgc ggtggcgcag tccgccgcgg ccgccgccat
    13621 gccgatggat tcgagcgccg cgatgcccgc gacgccggcc tcgtccttgc cgacgccggc
    13681 gtcgttcagg atcacggcgt gtaccgccgc gcgcgaggcg tagtaagcgg cgatgcgccc
    13741 gccgtgcgag ccggccacca gcacggcgcc gcggtggcgc gcgtcgagct cggtgacgct
    13801 atccgcgctg agcagggcgg gcacggtgca tttccacgca ggccgctgcg atcgcctgcg
    13861 gcgtgatgcg cgattcgtcc tcgagcggca gcgagtagcc gaccgggatg cgcggcgcgc
    13921 cgaggcgccg cacgcgtgcg ccgctctcct cggcgagcgt tgccgcgatc tcggcgccga
    13981 aacccgccac ctgcaccgcc tcgtgcgcga tgagcacccg gcccgtgcgc tcggccgagg
    14041 cgagcacctt gtcgcggtcc cagggccaga tcgtgcgcag gtcgatcacc tccagagaca
    14101 cgccgtcctt ctcggcgagc gccgccgctt cgagcgccgc gtggcgcgtc ttcgaccagg
    14161 tcaccacggt gacgtcgctc ccggggtgcg cgatctcggc ttcgcccagc tgcgcgcccc
    14221 cggccggcgc gacctcgccc tccagtcccc agagctcctt gtgctcgagg tagaccaccg
    14281 ggtcgccgct cgcgagcgag gcggcgagca gcgcgtggtt gtcctgtggc gtcgccggcg
    14341 cgaccaccac cagaccggga atgtgcgcga accacgcctc caggctctgc gaatgctgcg
    14401 cggccgagcc ggcccacagc ccgttcggca ggcgcgccac catgcccacg cggccctgtc
    14461 cgccgaacat gtagcgcgcc ttcgccgcct ggttgacgag ctcgtcgatg gcgcagagcg
    14521 cgaaatccac cacgcgcatc tccaccaccg gcttgaggcc cgcgagcgcc atgccgaccg
    14581 cggcgccgag gatcgtcgat tctgagatcg gcgtgtcgat gacgcgcttg gcgccgaagg
    14641 actgctgcag cccgcggtac tggccgaaga tgccgccgcg gccgacgtcc tcgcccacca
    14701 ccaccacccg atcgtcgccg cgcatggccg cctcgagcgc gctgcacgcc gcctgcgcat
    14761 aggtcatccg cgccatcggc cggctcccgt gtcctgcacg tcgctgtagg cgctggcctt
    14821 gccgggccag ggcgcctcat gcgcggcctt cagcgctgcg gcgacttcct cgcgcgcttc
    14881 ccggccgatg gcctcgagcg tctcgcgcat gttgatgcgc atcgccgcga gcaccagcgg
    14941 gtcgcgctcg agttccttgt cgtgcctggc cgcgtcgcga tagcccgcga catccaccga
    15001 tacatgaccc tttacccgat aggtgacggc gtgcaaaagc cgagggccgt cgccattgcg
    15061 tacttcggcg agcagcttcc cggcggcgca ccacaccgag aacacgtcgt tgccatccac
    15121 ctcgctgccg gggacgccga gggccgcggc gcgcgcgagc gcgcccgggc ccgcggtggt
    15181 cgcatcgctt gccgtggtgg ccgcccagcg gttgtcttcg cagatgaaga ggatcggcag
    15241 cttgtgcacg gccgcccagt tgagcccctc gaggaacggg ccgcggttga tggcgccgtc
    15301 gccgaagaag caggcgacga tgtcccgcgt gccgcgcatg gcgagcgcgt gcgccgcgcc
    15361 gaccgcgatc gggatgccgg cggcgaccac gccgttcgcg ccgagcatgc cgaccgagaa
    15421 gtcggcgatg tgcatcgagc cgcccttgcc gccgttgtag ccggtggcgc ggccgaagag
    15481 ctcgcacatc atgcgatcga gctgcgcgcc cttggcgatg gtgtggccgt ggccgcggtg
    15541 cgtggaggtg aggaggtctg tccgggcgag gtgcgcgcag acgccggcgg ccaccgcttc
    15601 ctggccggtg gacaggtgca gcggcccgcg cacctgggcc tgcgcgaggc tcgcaccgaa
    15661 cgcggcgacg ccgcccatcg aggcgtcctc ggcggcgtcc tcgaacgcgc ggatgcgcgc
    15721 catcgtgcgg tagagcgcga gcgcgcgccc gacgtcgggg gcctcctcca tgaggccaag
    15781 tctacgataa tgcccggatg ccgaagcggg ctcccctgac ggtcaacggc cgaagctacc
    15841 ggtggatgga ccggccgctc gccgcgatct gcgtcgacgg ctgcgagttc gactacatcc
    15901 acgaggcggt cgcggcgggc gtcgcgccgt accttggccg cgtgctcagg agcggcgccg
    15961 cgttcgaggc cgattgcgtg gtgccctcgt tcaccaaccc gaacaacttg tccatcgtca
    16021 ccggcgtgcc gccgtcggtg cacggcatct gcggcaacta cttctacgac cgcgaggcgg
    16081 atgcggaagt gatgatgaac gacccgaaat acctgcgggt cggcaccatc ctcgccgcct
    16141 tcgccaaggc gggggcctcg gtcgcggtgg tcaccgcgaa ggacaagctg cgcactttgc
    16201 tcggcaaaga cctcgatggc acctgcttct ccgccgagaa ggccgaccag gcgacgcaag
    16261 cgctcgtcgg caagccccag ccttcggtgt acagcgcgga gctgtccgag ttcgtgttcg
    16321 ccgccggcgt gaagctgatg gaaacgcgca agcccgacct catgtacctc tcgaccaccg
    16381 actacgtgca gcacaaggcg gcgccgggct cgccggcggc gaacgacttc tacgccatga
    16441 tggatcgcta cctggcgcgg ctcgacgaac tcggggcgac gatcgcactg acggccgatc
    16501 acggcatgaa cgacaagttc ggcgccgacg ggcagccgaa cgcggtctac ctgcaggacc
    16561 tgctggacga gtggctgggc gcgggccgcg cgcgcgtgat cctgccgatc accgacccct
    16621 acgtcgtgca ccacggcgcg ctcgggtcgt tcgccaccgt gtacctgccg tccgacgcga
    16681 acgcgagcgc cgtgatcgcg agcctgtcga aggtcgacgg ggtgcagatg gcgcttacga
    16741 acgcagacgc ctgcgcgcgc ttcgagctgc cgcccgagcg gctcggcgac gtgatcgtcg
    16801 tcgccgaccg gcacgccgtg ctcggcaagg gccgcgcgca ccacgacttg tcgggactgg
    16861 acgtgccgct gcgctcgcac ggcgggctgt ccgagcagac ggtgccgctc gtcttcaacc
    16921 gccgcaccgc cggcattccg ggcaagacgc ggatgcgcaa tttcgacgtc atcgacgtcg
    16981 cgctcaatca cctgggagcc tagccaccat gaaaatggcc ctggtcgtct gcaacagcta
    17041 cttcatggac cgcgtcatga aggtcctgga agataacggc atcgattact tcaccagctg
    17101 ggacaacgcc aagggcaagg ggcacggcac cgagccgcac ctgggcacgc gcgcctacgg
    17161 cagcacgaac tcggtgacga tgatcgcgtt cgaggacgag gcgccgctcg aggggctgat
    17221 tgccggcatc aaggaggaga accgggaaat ccgccgcgcg cccgaccaca tccggctgtt
    17281 cctgctgccg ctcgagcgca tcgtctgaag tggcggcggc ggcgcggacc gggccgcgcg
    17341 catcagtccg gcatcgtctg ccgccgaaag aaatcgagga tcacgcggtt ggcactccac
    17401 ccgccgacca tcgggccgat gaccctctcg cggaaatagg cccagccgcc gggccagacg
    17461 tggccgccgc cctccatgcg aatcagcacg gtgtccgctt ggcaccccgt ccaggccaat
    17521 tgaaccgtat tgacgcgtgg ttcgctgccc agcggccgcg gcgctcccgc ggccgcgcag
    17581 ccgttgatcg cggcccagcg acgcatcgac tcctcgaccg agatcttcgc cgtgtcatct
    17641 ctctgcgcgc acgcagcctt gccgccctcg tagcgccagc acgggtccgc agtaccgtgg
    17701 atctgcagga ccggcaccgg gcgcggcggc gcgcaggccg atacggtggc gaactgattg
    17761 gcgccgccca ccgcggcgat ggcggcgacg cgctccgcca actggcaggc cagccgatgg
    17821 ctcattgcgc cgccgttgga aaggcccgtg gcgtagacgc gccgcggatc gaccgcgacc
    17881 cagcgcgaca ggtcagcgag caacgcgcgg aaataggcga tatcgtcgac gccgtcctcg
    17941 caggcgcggc cgctcacgca ctgccagccg ttcgcgccgc cgccggcgtt ccaggtgcgc
    18001 acgttgcgcg caagtcgcgc gccggtgccg ttcggatagg ccacggccgc gccctcgcgt
    18061 cgcgccatcg cgtgcaggca gtcggcactg ccgacgtcgc cgccggggca ggcggtgcgc
    18121 gccgcagcgc ccgagttgcc gccaccgccg tggatcgcca gcaccaccgg aaccggtcgc
    18181 gccgggacgt aatcgacggg caggtgaatg tcgtaggggc gacgctcgaa gcccggaaca
    18241 tcgcaggtga gcgccgcacc cggcctcgcg tttgccggcg tgcaggtccg cggctgcgcg
    18301 gaggcgcagg cagaaaccgc aatcgacagc agtacgattt gaatcgggac gaagacgcgc
    18361 atccggctat tttgcgcgct tggcgatgtt ggaggtggcg ctgatcctca gttcgccgct
    18421 gccgcgatcg gggcggtgtc cgaggccgat cccggagccc gctcgctgcg gcgccactgg
    18481 ccgggggaga tgccgtaact ctgcttgaag gcgcggctga aggcggcttc cgattcgtag
    18541 ccgatgctgt cggcaatgtg gcccaggctg tggcgatcat gcttgagcat gcccgcggcc
    18601 atgctcaggc gccactgctt gaggtactgc atgggcgaca ccttgaccag ctcggcgaag
    18661 cgctcggcca gcacgctgcg cgacagattg accgcctgcg ccagctcggc cacggtccag
    18721 ctgcgggccg gatcggcgtg caacaacgac aggcacttgc cgatgtgcgg atcgcgcagg
    18781 cccgacagcc agccggcctg cgcgggcggc agcgactcca tgtagccgcg cagcgcttcg
    18841 acaaacagca gctcggcgac cttggccgcc acgacgtcga agccggcctg gtggccggcg
    18901 gcctgctcca gggcgtagcg gatcgactgc gcgatccact gccccgacgg acgcttgccc
    18961 agcgcagtga cgaactgcga cggcaggttg gcgacgagcg ggttgggcag ctcgcgctcg
    19021 taggcgaacc aacagcacac caggagcgcc tgctcgccgt cgccgccgta gcgcgccgga
    19081 ttgagctcgg gcattcgcgg ccgtaccacg tggtcgatgt gcgcggccgc gatctgcagg
    19141 ccgctgccca tgtggtgcga gtcaccgtgt gggtatacga cgatgtcgcc ctgctgaaga
    19201 tgcgtcggct cgccgccgct gagctccacc cagcattggc cttcgagcac gaagtggcag
    19261 atggcaagct gtttcgcgcc gggttgcagg acctgcgcca ggtcggcgcc ggtcggcacg
    19321 ttgaggcacc agggcgcgcg aaattcgccg ttgaggaaga gtgcgccgtg caggcgaatc
    19381 ttgcgcagca cgtcggacag tacatccatc cccgaccccc ggtggcactg ccaggcgttt
    19441 cggtcatcag atccggacga ccggacatga acgcaggcgg cgggcagcct agcatgaagg
    19501 ccgccaaatc gatcaacccg tcatcaaccg cgcagggaga ggcaatgaat ccaaacaagg
    19561 cactctggga aaagggcgac ttcacacgca tcgcggaggg catgcgcgag agcggagaag
    19621 cggtcgccag gaccttcggc atccgcccgg gcctcaaggt cctggatgtc ggctgcggtg
    19681 acgggacgac cgccctgccc gcggcgcaac agggggccga ggtgctgggc gtcgatatcg
    19741 cgaagaatct ggtggcggcc ggcaaccagc gcgccgcggc ggccggcctc acgaactgca
    19801 ggtttcagga gggcgatgtg agcgatatgc gggagctcgc cgatgattcc ttcgacctcg
    19861 tcgtctccat ctttggcgcc atgttcgcgc cgcggccgtt cgacgtcgcg aaggagatgg
    19921 cgagagtcgc gcggcccggg ggccgggtca tcatgggcaa ctggataccc aatgatccga
    19981 cgctggtggc gcagatcctg aagatcagct cggcctacac gccgccgccg ccggaaggct
    20041 tcgtcagccc gatgacctgg ggcgtggagg acaaggtgat cgagcgcttt gccgcagcgg
    20101 gcgtgtccgc ccaacagatc tcctttgccc gcgagacgtt cactttcaag accagcggcg
    20161 cgccgtccgc gtatctgcaa gcgttcaaga cctactacgg accgacgatg aacgcgttcg
    20221 aagccgcgga aaagaacggt cgggccgccg acctgcaggg ggaactcgaa gaattgttca
    20281 agagccagaa caagagcccg cgcgcggacg cgacctccat tccggcaacg ttcctgcgcg
    20341 tgacggtcgc acttcaataa aggagcagcc ccatgttcat gatcgactac aggcagcgcg
    20401 gctggtggta ctggctgctg accgcctgcc tgctcacggg gggcgtggcc ggctacccgt
    20461 tcggcttcga gctggccatc ggcctgaccg tggtgcacat gatcgactac atcgtgcgtg
    20521 agcgaagcct gatcgcattt acggtccagg tgcggctgag cgtactgctc tacctgctgc
    20581 tgtcgtaccc ggagccgttg cagttcctta tctgggtggc ggtcgtgggc atctgggccc
    20641 gggcgctgtt cggctactgc ctgatggcgc gcacgctctc gctcatgccc tggaatcgac
    20701 gggtgccgtt caccggcgcg ctgctgttca agacctactt ttcccgcccg gtgcgaggca
    20761 acatcatgca ggggctggca ccgctggcgc ccgcgctcgg cgcggcgacg ggcggctgaa
    20821 aacgaaagaa ggccgcgcca ggcggccttt gtcgttgtcg tggtggaggc ggcgggaatc
    20881 gaacccgcgt ccgcaagccc tagacagtca ggactacatg cttagcctgg ttatttgagc
    20941 ttcgccttga gaccgccaac cggcaggctg actcgcggct agccgcttcg acggatcccc
    21001 cgggccaagc ggccaagccc ggaagagagg ctgatgtgaa tgacgctgca ctccttttag
    21061 ggagcccggc ccatcagcag accggtgcag cgtctagccg gtgttaggcg gctagagcga
    21121 aacgctcgtc gttggcgttt gtagtgtttc cagatggatt tacgaggtca ctggtcctcg
    21181 gcatgccctg cgctgcgtcg caacccgcgt cgaaaccagg tcgcccccgt ttcgtgcgta
    21241 cgtgcattat cgcatatggg gtctagagca gcgcgatcaa gtccgccggc tgcgtgatca
    21301 ccgcgtcggc cttccaggag cgcgggccgc cgttctcggg atggtgatag ccccagccca
    21361 ccgcgatcgc gcgcatgtcc gcggcgcgcg ccgcctggat gtcgcgcagg tcgtcgccca
    21421 ggtacatgca ttcgcccgcc ggcagcttga gctgctcggc ggcgtggcgc atcggcgccg
    21481 gatgcggctt caggtgctcg gtggtgtcgc cgcacaccac gcaggcgggc ttcagcccca
    21541 gcgtctcgac gatgcgatcg gtgaagcgcg tcgccttgtt ggtgacgatg ccccaggcga
    21601 gcgagcgccg gccgagctcg gcgagcagct cggcgatgcc cgggaaaagc tgcgtgtgca
    21661 cgcaggtgtt ctcgcgatag gcctcgagga acgcctcgcg cagcgcgttg tagtcgtcat
    21721 cgcccggctt cacgccgaag cccgcgtgca ccaggccgcg cgcgcccgcc gaggcgaacg
    21781 ggcgcaggcg cgatagcggc accggctcca gcccctggtc ggtacgcagc cggttcagcg
    21841 ccgccgcgag gtcgggcgca gtgtcggcca gggtgccgtc gagatcgaac aggacggccc
    21901 ggctagccat cgcggcggca gcacagcagg tagttgacgt cgcagtcggg cccgaggcgg
    21961 tagcgctgcg tgaaggggtt gtaggtcatg ccgctgagcg cgacttcgcg caggcccgcg
    22021 tcgcggctcc agtgcgcgag ctcagacggg cggatgaagc gcgcgtagtc gtgcgtgcct
    22081 ttgggcagca gccgcagcag gtactccgcg ccgatgatgg cgaacaggta cgacttcggg
    22141 ttgcggttga tcgtggcgaa cacagcgcag ccgccggggc gcagcaaccg ggcgcaggcg
    22201 gccacggtgc ttgccggctc cggcacgtgc tcgagcatct ccatgcaggt cacgacatcg
    22261 aacgcgccgg cgtgcgccgc ggcgtagtcc tcggccgagg cgagctcgta gcgcacctcg
    22321 aggccgcttt ccatcaggtg cagccgcgcc acgctgagcg gcttctccgc gaggtcgatg
    22381 ccggtgacgc gcgccccgcg ctgcgccatc gcctcggcga ggatgccgcc gccgcagccg
    22441 acgtcgagca cgtccttgcc cgcgagcggc gcgtgatgct cgatccattc caggcgcagc
    22501 gggttgatcg cgtgcagggg ccggaactcg ccgtgcgggt cccaccagcg gtgggcgagg
    22561 gcgctgaatt tctccagctc ggcggggtcg acgttggggg cggcggtttg cattggcctt
    22621 taatacatct cggctttttc ggcgggaagg ttcaccccgc cgaaaaagcc gagaccatgg
    22681 gaaaagcgat gcggcacggc gcctatttta gacgccggcc tggcgtgttc ctactttcgc
    22741 cacttcgcga gccagtactc catctgcttg tccgccgctt cgcgcccgcc caggccgaag
    22801 gagagcgcca cggcgatcgc gcctaagatg ccggggatgt gctggcccag cgtgggttga
    22861 agcgaggaca agaactgttc catgatggcc ccctcgagct agtctttctg ggacggcgca
    22921 cacgatctga atcgcgggcg tcttgaaatt agttttctcg ggtgctgcgt cgacgcggcc
    22981 gcgagatgca cggcgccggc cgaagcgaac actatacccg cgtgggcgag ggagcgcatc
    23041 ccgtgccttc gttttgcgcc tttcaatacg tctcggcttt ttctggctgc gagattcatg
    23101 cagccagaaa aagccgagat catagaggac cgattgaggc actgcctttg tgcagcggca
    23161 gcgcttcacg ggcgacgagg cgaaaaaaag ccccgccgca gcggggcctt tcttcaccgc
    23221 ggtaacgcgg cttacttctt cgtaccgatg acttccagct ccacgcgccg gttcggctgc
    23281 aggcactcga tgagcgcctt gccgctcagc ttcgggcagg ccttcacggg ctgcgtctcg
    23341 cccttgccct cggtgtagat gcggttgggc tcgatgccct tggacaccag gtaggccttg
    23401 gccgcttccg cgcggcgcac cgacagcttc tggttgtact gcagcgtgcc gatcaggtcc
    23461 gcgtgaccga tggcgatcac cacctcgagg ttgatgtcgc ggatctgcgt cgacaggttg
    23521 tcgagccggt ccttgtagtc gggcttgatc accgacttgt cgaagtcgaa cagcacgtcc
    23581 gccgccagcg tcactttctg cgcgaccggc ttgggcgcgg gcttcggcgc aggcgcaggc
    23641 gcaggtgcgg gggccggcgc gggcgcgggg gccggcgcag gtgcgggctg cgcaggctgc
    23701 ggcgcggggg ccggcggcgg cggggcgggc tcctcggtcg tggcgcagcc aaccagaacg
    23761 gccatcgcga ccagtgcagc gctcagacca gccgatcgaa aaatgttttt cggcattcgt
    23821 tttctccagt tgcctagggg attgtcgggg ttgctgatcc ggacccaaag gtaaaggccg
    23881 gtcaactcaa ggaattcaat attttagttt gccacaaggc tgctcggcct tgcaagtcag
    23941 cggcgtcgat gcgcgtgagc cgtcgatcgc gcagcacgcg ctcgccgccg acccagacgt
    24001 cggtgacgtg ctcgcgcccg cacgcgtaca cgaggtggga gacgacgtcg tacaccggct
    24061 gcagcgcgac gtcgtcgagc gccaccgcgg ccagatccgc ggccttgccc gggacgatgc
    24121 tgccaaagcg cgcctcggcg ccgagggcga tcgcgccgcc gagcgtcgcg gcgtgcagcg
    24181 ctgcgtgcgc cggcatcgcc tcggcgtcgt cgcccgccgc cttggcgagg agcgccgcca
    24241 gacgcatctc ctcgaacatg tcgagccggt tattgctggc ggcgccgtcg gtgccgaggg
    24301 cgatgttgac gtccgccgcg agcagttgcg ccaccggcgc gaggccgctc gcgagcttca
    24361 ggttcgacga ggggcagtgt gcgaccgaag cgcgatgccg cgaaagcaga gcgatttccg
    24421 gcggcgacag atgcactgcg tgcaccgcga tcagtcccgg gccgagcagc cccaggccgg
    24481 ccaggcgctc gaggggccgc atgccgtgct cggccatcga gcgctcgact tcgcccgcgg
    24541 tctcgtgcac gtgcacgtgc accggcagct cgagctcggc ggcaagctgc gccaccctgc
    24601 ggaaggtcgc gtccgacacg gtatagggtg cgtgcggcgc gaggcagaag gcggcgagcg
    24661 gatgctcgcg ccagcggtcg cgcagcgcca ggccctgcgc cagataggac tgcggatcgg
    24721 aagcgtacgc ggacgggaac tcgatcacga tcaggccgag aaaggcgcgc atgcccgcct
    24781 gcagcgccgc ttccagcgcc gcttcgggaa aatagtacat gtcgttgaag cacgtcactc
    24841 ccccgcgcag catctcggcg cacgcgagga gcgtgccgtc cttcacgaac tcagccgaga
    24901 cgtgctttgc ttccactggc cagacgtgct cctcgagcca gcgcatcagc ggcaggtcgt
    24961 cggccatccc gcgcatcagc gccatggcgg cgtgcgtgtg cgcgttgacc aggcccggaa
    25021 tcagcgcgtg cccgggaagt tcgatgcgcg cgtagtctgg atagcgctcg gcggcctggg
    25081 ccgcgggcaa cagtgcttcg atacggccgt cgcgcaccgc gacggcgtgg cgctcgagca
    25141 ccgtgccggc gggctcgacc ggtaccaccc agcgcgcgtt gatgagcagt cgttccgtca
    25201 tgcgatgcgg cgccaacaaa aaagccccgc acatggcggg gcttggttgg aggcgggcgg
    25261 cgcctagcgg cccgtgccca ggacctcgac ttctgcgcgg cggttcggct gcaggcaggc
    25321 gatcagcgcc ttgcggtcct tctggtcgca gctcacgacc gggagcgtcg agccgacgta
    25381 catcttgccg tagccggtgg tctggatctt ggagccctcc gcgccctggc gcacgaggta
    25441 ggccttgacg gcctccgcgc ggcgctcgga gagcttctgg ttgtactgca ccgagccgat
    25501 gcggtccgtg tagccgttga tgttgataaa gcgcacgtcg ctgaattcgc cgaagcgcac
    25561 caccacctcg cggtcgagcg tgtcctgcgc gcccatgctg agcaccgact tgtcgaacgc
    25621 aaacagcaca tgcgagaaca cgcgcagcgg cttgggcttg gcgggctcgg cgaccggctt
    25681 gggcgcgggc gctggagccg gcgcgggtgc cggggccggc gcggccgggg ccggggccgg
    25741 cgtggccgga gccggcgcgg gtgcgggcgc gactggcgcc tcttcggtgg tggcgcatcc
    25801 caccatcgcg gcgagcgcag tggcgcagac aaaaagctgg gcagttctcc tgttcatgct
    25861 tgttctccct cgcacggaat gccccgcatt taagcacagc ctcgcgccga ggtcgagagg
    25921 tctcgcgtgc taaaatttca cgctcgaaac agcgccttaa acggccactt tccctcccag
    25981 aacaagagcc gcaatggaac cgttcgccaa ggaaacgctc cccgtcagtc tcgaggagga
    26041 gatgcgccgc tcgtacctcg attacgcgat gagcgtgatc gtggggcgcg cgctgccgga
    26101 cgtgcgcgac ggtttgaagc ccgtgcaccg gcgcgtgctc tacgcgatgc aggaatcgaa
    26161 caacgtctgg aaccggccct acgtgaagtg cgcgcgcgtg gtcggcgagg tgctgggcaa
    26221 gtatcacccg cacggcgact cggcgaccta cgaggcgctg gtgcgcatgg cgcaggactt
    26281 ctccatgcgc tacatgctga tcgacgggca gggcaatttc ggctcgatcg acggcgacaa
    26341 cgcggcggcc taccgctaca ccgagtgccg cctggcgcgg ctctcggccg agatgctggc
    26401 cgacatcgac aaggaaaccg tcgatttcgt gccgaattac gacggcaagg agctcgagcc
    26461 cgcggtgctg ccgacgcgcg tgccgaacct gctggtcaac ggctcctcgg gcatcgccgt
    26521 cggcatggcg accaacgtgc cgccgcacaa cctcggcgag gtcgcggacg cctgcctgca
    26581 cctgctcgag aacccgcatt gcgcgatcga agacctgatg aagctgatgc cggcgcccga
    26641 tttccccacc gcgggcatca tctacggcct ctcgggggtg cacgagggct accgcaccgg
    26701 ccgcgggcgc gtggtgatgc gggcgcgcac gcacttcgag gacatggaaa aaggcaatcg
    26761 ccaggcgatc gtggtggacg agctgcctta ccaggtgaac aagaaggcgc tgctcgagaa
    26821 gatcgccgag ctcgtcaccg agaagaggat cgagggcatc tccgacatcc gcgacgagtc
    26881 cgacaaggac ggcatccgcg tggtgatcga gctgcggcgc ggcgagctgc cggaagtggt
    26941 gctcaacaac ctctacaagc agacgcagct gcaggactcc ttcggcatga acctcgtggc
    27001 gctggtcgac agccagccga agctcctcaa cctgcagcag ctgctcgagg cgttcgtctc
    27061 gcaccggcgc gaggtggtga cgcggcgcac cgtgttcgac ctgagaaagg cccgggagcg
    27121 cggccacgtg ctggaagggc tcgcggtggc gctctcgaac gtcgacgagg tcatcgcgct
    27181 gatcaaggcg gcgcccgccc cggccgacgc caagcgcgag ctgatggcgc gcgcctggga
    27241 ttcggcgctc gtgcgcgaga tgctctcgcg cggctcggcg caggcctacc ggcccgaggg
    27301 cctggccgcg gagttcggcc tgcgcgagga cggctaccgg ctctcggacg cgcaggcgca
    27361 ggcgatcctc gagctgcggc tgcagcgcct caccggcctc gagcaggaca agatccgtgc
    27421 cgagtacaag gaggtcatgg agcagatcgc cgacctgctc gacatcctgg acaagcccgc
    27481 gcgcatcact gcgatcatcg gcgcggagct gaagaagctc aaggaggact tcgccgaccc
    27541 gcggcgcact gaaatcgtca cggtggccga ggacatctcg atcgaggacc tgatcgcgcc
    27601 gcaggacatg gtggtcacgt tctcgcacgg cggctacgtc aaggcgcagc cgctcgccga
    27661 ttaccgcgcg cagcgccgcg gcgggcgcgg ccgcgccgca actgcgatga aggaggacga
    27721 cttcatcgaa cggctgttcg tcgcgcactc gcacgactac ctgctgtgct tctccagccg
    27781 tgggatcctg tactggctca aggtgttcga ggtgccgcag ggcagccgcg ccagccgcgg
    27841 caagccgatc gtcaacgtct tcccgctgca ggagggcgag aagatcaccg cggtggtgcc
    27901 ggtgaaggaa ttcgacgagc agcacttcgt gctgatggcg accgcgcgcg gtacggtgaa
    27961 gaagacgccg cttgccgagt tctcgcgccc gcggcaggcg ggcatcattg cggtgagcct
    28021 ggaagacggc gactacctcg tgggcggcgt gctcaccgac ggcagcgcgg acgtgatgct
    28081 gttctcctcc gagggcaagg cggtgcgctt cgccgaatcc gaggtgcgcc cgatggggcg
    28141 gcaggccgcg ggcgtgcgcg gcatgcgcct gcccgaggac ggcagccagc gcgtggtgtg
    28201 catgctggtg gcgcgggacg agagcttgag cgtgttcacc gccaccgagc acggctacgg
    28261 caagcgcacg cccatctccg agtaccccaa gcacggccgc ggcgggcagg gcgtgatcgc
    28321 gatccaggcc tcggagagga acggccgcct ggtcggcgcg gtgctggtgg acgacagcga
    28381 cgaggtgatg ctgatctcga cgcgcggcgt gctgatccgc accacggtgg cgcaggtgcg
    28441 cgcgcagggc cgctcgacgc agggcgtgac gctgatcgcg ctgggcgagg gcgagaagct
    28501 cgccgggctc gagcgcatcg aggaaaaaga cgaggacgtg cccggcaacg gcaacggcaa
    28561 cggcgacggc ggggaagcgc cgctgccgcc ggcagaggaa ctgccgccca cggttcattg
    28621 atgtcgcgcc ttttcaactt cagcgccggg ccggcgatgc tgccggccga ggtcctggcg
    28681 cgcgcgggcg acgagatgct cgactggcgc ggcagcggca tgggcgtgat ggagatgagc
    28741 caccgcggca aggagttcat ggcgatcgcg gcggaggccg aggccgacct gcgcgagctg
    28801 gtcgcgatcc ccgcgcacta caaggtgctc ttcctgcagg gtggcgcgac gctgcagttc
    28861 gcgcaggtgc cgatgaacct gctgcgcggc agatcgggtg cggactacgt cgtcaccggc
    28921 gagtggtcga aaaaggcggt gaaggaggcc aaggcctact gcgacgtgca cgtcgcggcg
    28981 agctcggaag accgcaagtt cgcctacgcg ccgcgcacct ggaacgtgcg ccaggatgcc
    29041 gcctacgtgc actactgctc gaacgagacc atcggcggcg tcgagttcca ctgggtcccg
    29101 gagacgggcg cggtgccgct ggtcgccgat gcctcctcgc acttcctgtc gcggccgctg
    29161 gcggtggaga agttcgggct gatctacgcg ggcgcacaga agaacgccgg gccggcgggc
    29221 ctcacgatcg tcatcgtgcg cgaggacctg atcgggcacg cggcgaaggg cacgcccacg
    29281 gtgatggact acaagctgca ggccgacgcc ggctcgatgc tcaacacgcc cgccacctat
    29341 tcgatataca tcgcgggcct ggtgttcaag tggctcaagc agctcggcgg gctcgccgag
    29401 atcgagcggc gcaatgtcgc caaggcgcgc ctgctgtacg aggtgatcga cggcggatcg
    29461 ttctacggca atcccgtcga caaggccgac cgctcgcgca tgaacgtgac cttccggctg
    29521 cccgacgaac ggctcgatgc gccctttctc aagggcgcgg aagagcgcgg cctgtcgcag
    29581 ctcaagggcc accgcgcggt gggcggcatg cgcgcctcca tctacaacgc catgccgccc
    29641 gagggggtcg agcagctggc gggctacatg cgcgagttcg cggcgcgcca tggctgagcg
    29701 agccgggaag ctcgaggtgc tggtgctgaa ccgcgtggcg caggcgggcc tgcagcgctt
    29761 tccggccgag cgctacgcgc tggtcaccga agcgagcgcg ccgcacgtgg tgctcgtgcg
    29821 ctcgcaggac ttgcaccgcg ccgcgctcgg cgagcgcctg caggcggtgg gacgggccgg
    29881 cgccggaacc aacaacatcc cggtgggcgc gctctccaag cggggcgtgc ccgtattcaa
    29941 cacgccgggc ggcaatgcca acgcggtgaa ggagctggtg atcgcgggcc tgctgatggc
    30001 ggcgcgcaac ctcgcgccgg cgctcgattt cgtgcgcgga ctggacaagg cggcgccgga
    30061 cttcgagcgg cgcatcgagg acggcaagaa ggcgttcgcc gggggcgagc tccccggcca
    30121 gacgctcggt gtggtcggcc tgggcaagat cggcagcctg gtggcggacg ccgccatccg
    30181 gctcggcatg caggtgatcg gctacgaccc cgacatcacg gtggaagggg cctggagcct
    30241 gccgtcgcag gtgcgccggg ccgcctcggt ggacgagctg atgcgcgcga gccgcttcgt
    30301 gacgctgcac gtgccgctcg cggagcgtac gcgccacatt gtcaatgcga agaacgtcgc
    30361 gctgatgcgc gagggtgcca tcctgctcaa cttctcgcgc gagggggtgg tggacgacgc
    30421 cgcggtgctg gcggcgctcg ctgccgggcg gctgcgcggc tacgtcaccg acttcccgac
    30481 gccggcactg gtcggcgccc ccggggtggt ggcgctgccg cacctgggcg cctcgacgca
    30541 ggaagccgag gaaaactccg cggtgatggt ggtggaccag gtgcgcgagt acctcgagca
    30601 cgggaacctg cgcaactccg tgaactttcc cgaggcggtg atggcgcgcg agtcgcccta
    30661 ccgcctcgcg atcgccaacg ccaacgtgcc cgacatgctg gcgcgcatct cccacgccat
    30721 gggcgagcgc ggcatcaaca tccacaacat gctgaacaag tcccggggcg acgtggccta
    30781 cacgctggtc gacgtcgaca gcccggtgcc ggaggccgcg ctaagcgcgc tgcgcgccat
    30841 cgacggcgtg ctcgcggtca gatatcttcc gctcgggcca tgagcggcga catcgacaag
    30901 ctgcgcaccg agatcgacgc gctcgacgcg gagctggtcg cgctgctgca gcggcgcgcg
    30961 cgcctcgcgc agcgcatcgg tgcggcgaag ggcggcgcgg cggcctaccg gccggagcgc
    31021 gaagccgaga tcctgcgccg cgtcgcggcg cagggcgggg agccgctgcc cgcggctgcg
    31081 ctgacgcgcg tgttccgcga ggtgatctcg gcctgccgcg gcctcgagca ggagatccgc
    31141 gtcgcttacc tggggccgcc gggcaccttc agcgagcagg cggtgcgccg ccatttcggc
    31201 ggcagcgtcg agggcgtgcc ggcggcgagc atcgacgagg tgttccgcag ctgcgagtcg
    31261 ggcgctgcgc agttcgccat ggcgccggcg gagaactcga ccgaaggggt ggtgggccgc
    31321 acgctcgatc tgctgctggt cacgccgctc aggatctgcg gcgaggtgga gctgcgcgtg
    31381 cagcagaacc tgatggcaca gcacgccgac agctcgcgcc tgaagcgcgt gtactcgcac
    31441 gcgcagtcgc tcgcgcagtg cagcgcctgg ctcaatcacc acttgccggg cgccgagcgc
    31501 gtgccggtcg cctcgaacgc cgaggccgcg gagcgcgccg cgcgcgagcc gcacgccggc
    31561 gcgatcgccg gcgaggccgc cgccgagcgc tacgggctgc agctgcttgc gcgcgcgatc
    31621 gaggacgacc ccaacaacac cacacggttc ctggtgctgg gcacgctcga ccccgcgcca
    31681 accggccgcg accgcacctc gctcgtcatg tcggccgaga acaagcccgg cgcggtgcac
    31741 gcgctgctca cgccgctcgc cgagaacggc gtcagcatga cgcgcatcga gtcgcgtccg
    31801 gcgcgcgcca tgcgcagcgc gctgtgggag tacgtgttct tcatcgacct cgaggggcac
    31861 gcaagcgaag cgcgcgttgc cgccgcgctc gaggcgctgc ggcgcaaggc gccgtttctc
    31921 aaggtgctcg gctcctaccc ggctgccggc acgtaaagga ccgaccgaca tgatcatgga
    31981 cccctgcgag agctcgccct cgtacgtgcg ctcgatcgcg ccctaccagc ccggcaagcc
    32041 gatctcggag ctggcgcgcg agctcgggct gaaggaggag acgatcgtca agctcgcctc
    32101 caacgaaaac ccacgcggca tcggcccgcg cacgcgcgcc gccatcgagc gcgcgctggg
    32161 cgaggtcgcg cgctacccgg atggcaacgg ccacgcgctg aagacggcgc tcgcggcccg
    32221 ctacggcgtc gacatggcgc agatcgtgct cggcaacggc tcgaacgacg tgctcgagct
    32281 ggtggcgctc gctttcctcg gaccgggccg cgccgcggtg tactcgcagc acgcgttcgc
    32341 ggtctatccg ctcgccaccc aggcgcgcgg cgcgcgcggc atcgtcgtgc cggcgcgcga
    32401 ctacgcccac gacctggaag cgatggcgcg cgcggtggac gacgacacgt gcgtgctgtg
    32461 ggtcgccaac ccgaacaatc cgaccgggac ctacgccacg ggcgatgcgc tggaggcgct
    32521 cgtacgccgc gtgcccgagc gcgtgatcgt ggtgatcgac gaggcctaca acgaatacct
    32581 gcccgcggac ctgcgcgtcg acaccgtcaa gtggctcaag cgccattcca acctcgtcat
    32641 cacgcgcacc ttctccaagg cctacggcct ggcggggctg cgcgtcggct acgcgctcgc
    32701 gcacccgtcg gtggccgacg tcatgaaccg cgtgcgccag ccgttcaacg tcaacagcat
    32761 cgcgctcgtg gccgccgccg cggcgctcga agacatggag ttcgtggcga agagcttcgc
    32821 cggcaacctg cagggcatgc gccagctgcg cgacggcgcg cagcagttgg ggctcgcctt
    32881 catcccctcg tacgcgaact tcctcaccgt gcgcgtcggc aaggcgcagg acatctacaa
    32941 gcggctgctc aggcgcgggg tcatcgtgcg cccggtgggc ggcggctacg agctgcccga
    33001 gcacctgcgc gtcaccgtcg gcacggaaga ggaaaacgag aggttcctct cggcgctggc
    33061 cgcgagcctg aaggactgat gcactttgcc aaggtcgccg tcgtcggcgt cggcctgatc
    33121 ggcggctcgt tcgccctcgc gttgaaggcg gcgcgagcct gtggcgacgt ggtgggcgtg
    33181 ggccgcggcg cggccaacct gaagctcgcg ctggagcaag gcgtcatcga tgccgtcggt
    33241 gcggatatcg cggatgccga cctggtcctc gttgcgactc ccgtggcgca gtacccgcgc
    33301 gtgctcgccg cgctggcacc ccgcctcaag gcctcgacga tcgtcacgga cgcgggcagc
    33361 accaagcgcg acgtgatcgc ggccgcgcgc gccgcgctcg gcgcgaagat cgcgcagttc
    33421 gtgcccgcgc acccgatcgc gggcgccgag cgtagtggcg cttcggcggc aagcgcagag
    33481 ctgttccgca ataggcgcgt ggtcctcacg ccgttacccg agaacccggc cgcagcggtg
    33541 caggcggtcg aggcggcgtg gcaggcgtgc ggtgcgcgcg tctcgcgcat ggaggcggcc
    33601 gaacacgacg ccgtgctcgc cgcggtgagc cacctgccgc acctgctcgc ctacgcgctg
    33661 gtgcacgagg tggcggggcg cgcggactcg gcacagctgt tcggttttgc cgcggggggc
    33721 tttcgcgatt tcacgcgcat cgcctccagc caccccgaga tgtggcgcga catctgcgtt
    33781 gcgaaccgcg accggctcct cgacgagctc gaccgctacc ttgccaagct gcaggccgtg
    33841 cggccgctcg ttgaaaacgg cgacggcgcc gcgctcgaga agctctttgc cgaggcgcgc
    33901 gctgcgcgcc agcgctggct cgccggagag tacgagtgaa gacgctcgat ctcgggccgc
    33961 tcagccgtgc ggcgggcacg gtgcggctgc cgggatcgaa gagcatctcc aaccgcgtgc
    34021 tgctgctcgc ggcgctggcg cgcggcgaca ccgaggtgcg tggcctgctc gacgccgacg
    34081 acacgcaggt gatgcgcgcg gcgctcggca ggctcggccc gcagctcagc gtcaagcagg
    34141 ccgaactcga tctcggcaac gccggcaccg cgttccggcc gctcaccgcg gtgctcgcgc
    34201 tctcgggcgg tgaataccgc ctctcgggca cgcggcgcat gcacgagcgc ccgatcggcg
    34261 atctggtgga tgcgctgcgc gcgcttggcg cgcgcatcga ctacctgggc gcggaaggct
    34321 ttccgccgct cgccatccac cccggcaagg tggcctccgg ccagaccgtg agagtccgcg
    34381 gcgacgtgag ctcgcagtac ctgagtgcgc tgctgatggc gctgccgctc gccggtggcg
    34441 gcacgatcga agtcgcgggc gagctgatct cgaagccgta cgtggagatc acgctcaatc
    34501 tcatgcggcg cttcggcgtc gacgtgacgc gcgacgcctg gcgccgcttc atcgtgccgg
    34561 ggacgcgcta ccgctctccc ggaagcgtcc aggtcgaggg cgacgcctcg agcgcctcgt
    34621 atttcctcgc cgccggcgcg atcggtggcg gcccggtgcg cgtcgagggc gtcggtcgcg
    34681 acagcatcca gggcgacgtc gccttcaccg gggtgctcga gcgcatgggg gccgcggtca
    34741 ccct
//
LOCUS       DBNHKE010000161        11273 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_451173, whole genome shotgun sequence.
ACCESSION   DBNHKE010000161 DBNHKE010000000
VERSION     DBNHKE010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_451173"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..995)
                     /locus_tag="AC16O9_10950"
     CDS             complement(69..995)
                     /locus_tag="AC16O9_10950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6517173.1"
                     /translation="MKIAIVGCGAMGSVYAGLFASAGHEVWAIDRWAEHVEAMRRNGL
                     RLEGKSGDRTVKVHATTDAKDAGTCDLVVLATKAMHVAPAAESIKALLGKDTPVLSIQ
                     NGLGGPETAAEILGRERVMVGVVGGFGASMKGPGHAHHNGMELVRLGEFGGPITPRLR
                     KVEETWAGAGFKVKVFDDIDQLIWEKLICNCAYSGPCGVMEYTIQQVLDDPEMSKVSA
                     ACAAEGYAVAKKKGVKLDFSDPVAYVRDFGAKIPNSRPSVLLDLMAGRKSEIDVINGS
                     IPRVGKQVGVAAPVNETITALVKAREAKLGCK"
     gene            complement(1021..2199)
                     /locus_tag="AC16O9_10955"
     CDS             complement(1021..2199)
                     /locus_tag="AC16O9_10955"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR6517174.1"
                     /translation="MTEAVIVSTARTPLCKSWRGALNMTHGAKMGGHVVHAALERAKV
                     DADEVEDVIIGCATPEGATGGNIARQIAIRAGCPVTVPGMTVNRFCSSGLQTIALAAQ
                     RVLAGEGTVFVGGGVESISLVQNEANKFHAQDEWIHRHKPEIYWPMLQTAEYVSKKYN
                     ISRELQDRYGARSQQRAAKARAEGKFKDEIVPITAKMKVIDKNTNVETMREVTVEHDE
                     GIRPDTTYEGVSQIKPAIEGGVIAAGNASQFSDGASAAVVMSDKEAAKRGLKPLGVFR
                     GFAVAGCEPNEMGIGPVFAVPKLLEMAGKKVSDIDLWELNEAFAVQVIYCRDKLGIPD
                     DRLNVNGGAIAVGHPYGVSGARLVGHALIEGKRRGAKLVVVTMCIGGGMGAAGLFEVA
                     "
     gene            complement(2211..4298)
                     /locus_tag="AC16O9_10960"
     CDS             complement(2211..4298)
                     /locus_tag="AC16O9_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR6517175.1"
                     /translation="MSDVAAYSVRDGIAVITLNNPPVNGLGNALRAGIAAGLEKADSD
                     PDIKAVVLIGSAKAFSGGADIREFGKPMTKPILPEVNDIQDSMQKPLVAAIGGFALGG
                     GLELALGCHYRVALEKSLLGLPEVKLGILPGSGGTQRLPRIIPVHEAARMMILGDPIP
                     APKGKELGLIDEIVSGDLLEAGIAYAQTLVAAGKGPRRIRDMPPKVEGDQKALLEAVP
                     EQAAKSAGGNPAPAEIAKCIHAAVTLPFDEGRKVERERFEYLVNTVESKALRHMFFAE
                     RQTSKIPDVPESTPTREIRKAAVVGAGTMGGGIAMSFANAGIPVVIADVSQEALDKGM
                     KKIRDNYEGTVKRGRLKPEEMEKRYALIQPSTDLNALKPADIVIEAVFERMDVKQDMF
                     KKLDALMKPGAILATNTSTLDVNKIADVTKRPQDVIGTHFFSPANVMRLLEVVRGEKT
                     AKDVLATTMKLGKKLKKVPIVSGVCDGFIGNRMVERYSQQAGLLLDEGASPQQVDAAL
                     QKWGMAMGPFTMSDLAGNDIGWDIRKRRYYERPEMAYSKFADKVCELGRFGQKTGKGF
                     YRYEPGNRKALPDPEIDALLDKYRKEHGLQTRAISDQEIVERCIYALVNTGARILEEG
                     IALRASDIDMVYLAGYGFPPHRGGPMFYADCVGLDKVVVAIERFQKGYHGEQWQLAPL
                     LVKLAKEGKRFNG"
     gene            4412..6040
                     /locus_tag="AC16O9_10965"
     CDS             4412..6040
                     /locus_tag="AC16O9_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HIR6517176.1"
                     /translation="MHGLMMDVPLLITDLIRFADRNHGDTEIVSKTVEGELHRTTYRD
                     AHARARRLAKVLQALGVKASDRVGTLAWNGYRHYELYYAISGIGAVINTINPRLFPDQ
                     IVYISNHAENQALFFDLTFVPLVEKLAPQLKTVKHFVLMTDRAHMPAKSPIPNLLCYE
                     ELMAKQDERFEWPSFDERTAAALCYTSGTTGNPKGVLYGHRSTVLHAYGAALPDALNL
                     SAGDSVLPVVPMFHANAWSLPYACTMVGAKMVFPGPHLDGKSVYDLIEQEKVTLSAGV
                     PTVWLMLLQHLTAHKAKFSTLKRTVIGGSACPPAMIRAFQDDYGVTVFHAWGMTEMSP
                     LGTVGSLKAKQAALPKDARYAVQAKQGRGIFGVEMKIVDEEGKELPWDGKRFGNLLVK
                     GPWIASGYMKGEGGDPLKDGWFPTGDVATIDPEGYMQITDRSKDVIKSGGEWISSIDL
                     ENIAVAHPAVQEAAVIGIRHPKWDERPIVVAVRKSGQQVTKEDLLKFYEGKIAKWWMP
                     DDVVFVDELPHTATGKLSKLTLREKFKDYRLPGA"
     gene            6037..6804
                     /locus_tag="AC16O9_10970"
     CDS             6037..6804
                     /locus_tag="AC16O9_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6517177.1"
                     /translation="MSHVLVETRERVTRIELNRPEKKNALSAEMYAAMAKALAAAEAD
                     AQVRAVLIHGQADCFTSGNDVKDFLELPRGPGDSPATGFLRAISAAKKPLVAAVGGAA
                     IGIGTTMLLHCDLVYAAPNARLQMPFVPLGLLPEAASSLLLPAMAGYRRAAEMLLLGG
                     PFGADQALAAGIVTEIVPQTELLERARQAALALAALPPASVRMTKELMKRGLAAAVQE
                     RMTEELGLFGERLRSPEAKEALSAFLEKRRPDFSRFS"
     gene            6801..7667
                     /locus_tag="AC16O9_10975"
     CDS             6801..7667
                     /locus_tag="AC16O9_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517178.1"
                     /translation="MSLVHETVAANGLKFHVARAGEGPLMLFLHGFPEYWAMWRPLLE
                     HFGARGFCAAAPDLRGYNLSDKPAAVEAYRAKHLMADVLALAAHYTNEKFVLVAHDWG
                     GAVAWGVAIAHPERLAKLAIVNAPHPYLFWRELCTSPAQQKASEYMNFFRLAKAERVL
                     AENGYARLLGAFSDLDEAARTALVEAWSQPGALTGGLNYYRASPMYPPSADDPGAKKL
                     QLKPEDFVVRVPTLVVWGERDTALLPGCIDGLEAVVPDLKLVRVPEASHWIARERTDL
                     VIREIGAFTIGR"
     gene            7664..8116
                     /locus_tag="AC16O9_10980"
     CDS             7664..8116
                     /locus_tag="AC16O9_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6517179.1"
                     /translation="MKTSLRIRDLESRVGQEIAQSPWVEMPQERIDLFAKATEDYQWI
                     HVDPERAKASPFGTTIAHGFLTLSMLPKLIESTFEFSDRKMGVNYGLNKVRFTSPVPA
                     GAKIRGRFTLAKYEKLDGGVQTTWSVTVEREGGEKPAMVAETISRHYF"
     gene            8118..9161
                     /locus_tag="AC16O9_10985"
     CDS             8118..9161
                     /locus_tag="AC16O9_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6517180.1"
                     /translation="MTQRWKHKPPNSNWGEFGPDDQRGRMNYVTREKVLQGVAEVKEG
                     IAFCLSLPLDYPGGNALNPRRRPPRLAPTIRDGKQAFCLPLAEFVGDVTDVVCDDQVL
                     LALQYSSQWDSFAHVGAKFDADGDGKAEIVFYNGFRAGEHVKPGPEKPDAEPWAKFEG
                     ARAEALGIQNLAEHGAQGRGVLVDLHAHFGRERKAIGYDALMRVMEKDKVVVEKGDFV
                     CFYTGFADVLLELKKNPDPQLIHNTCTGLEGRDDRLLQWITDSGCAALIADNYAVEII
                     PASPFKPLPHALMPLHEHCLFKNGIHLGEIWYLTELARWLREHRRNRFLLTAPPLRLP
                     GAVGSPAPAVATV"
     gene            9172..9648
                     /locus_tag="AC16O9_10990"
     CDS             9172..9648
                     /locus_tag="AC16O9_10990"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR6517181.1"
                     /translation="MADNDRRDLVAQMKNRALIYKETYDVLAEELGAAKAEALLTRAI
                     YQRGRAMGKSFAKYAPADLNGLREAFLASVPGGADIFQPEVLKKEAATLEIQLHGCPL
                     KDAWREAGLPEDKVATLCRIAGAVDKGLFEGAGFKIENRTWTPGAKGCCFLRILRG"
     gene            9641..11236
                     /locus_tag="AC16O9_10995"
     CDS             9641..11236
                     /locus_tag="AC16O9_10995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6517182.1"
                     /translation="MAEHWDYIVVGAGTAGCVLANRLTEDGRHRVLLLEAGPRDTNPW
                     IHVPLGYGKLFTRTDVNWAYESEPEPTLNGRRIFTPRGKVLGGSSSINGLVYIRGQRE
                     DFDGWGLAGWRFDELLPYFKKSEDQGRGADAWHGVGGPIGVNDLPDRHELCDAFIASA
                     EALGVPRNDDFNGARQEGAGYYQATTRNGRRSSAATGYLRPARKRANLRVETEALATR
                     VLFDGTRAVGVEYSQGGATRQAHVAREVILAGGSINSPQLLQLSGVGPRALLERHGIR
                     PLHDAPGVGEDLQDHFYVRTFWRCTKPITLNDDMASWWRQLGIGLRYVLQRRGPLTVS
                     AGYAAAFVRTRPELKRPDGQIYFINFSTAKRGGVLHPFPGFTLSLSQLQAESRGWVRI
                     RSADPKAPPAIHYNYLSTEKEQRTMVDGLKLVRRLAQTPPLAGYVAEEFQPGPKVQSD
                     ADWLAYGRDNGDTVFHPTSTCRMGEDARAVVDARLRVRGLQSLRVIDASVMPAVVSGN
                     TNAAVFAIAEKGADLVRADANRA"
BASE COUNT         1734 a         3892 c         3839 g         1808 t
ORIGIN      
        1 aaatagggac agacctcatt tatccagtct gcgcttggtg cgcagacgtc gacaaatgag
       61 gtctgtccct atttacagcc gagcttggct tccctcgcct tgaccagcgc ggtgatggtt
      121 tcgttcaccg gcgcggcgac gccgacctgc ttgccgaccc gggggatcga gccgttgatg
      181 acgtcgatct cggacttcct gcccgccatc aggtcgagga gcaccgaggg gcgcgagttg
      241 gggatcttgg cgccgaagtc gcgcacgtag gcgaccgggt ccgagaagtc gagcttcacg
      301 cccttcttct tcgccaccgc gtagccctcg gcggcgcagg cggcggacac cttcgacatc
      361 tccgggtcgt ccagcacctg ctggatggtg tattccatga cgccgcaggg gcccgaatag
      421 gcgcagttgc agatcagctt ctcccagatc agctggtcga tgtcgtcgaa caccttgacc
      481 ttgaaccccg cgccggccca ggtctcttcg accttcctca ggcgcggcgt gatcggcccg
      541 ccgaattctc cgaggcgcac gagctccatg ccattgtggt gcgcgtgccc ggggcccttc
      601 atcgaggcgc cgaagccgcc caccacgccc accatcacgc gctcgcggcc gaggatctcg
      661 gccgcggtct ccgggccgcc gaggccgttc tggatcgaaa gcaccggcgt gtccttgccg
      721 aggagcgcct tgatcgactc ggcggccggc gccacgtgca tcgccttggt ggcgagcacc
      781 acgaggtcgc aggtgccggc gtccttcgcg tcggtcgtgg cgtggacctt gacggtccgg
      841 tcgcccgact tgccttcgag ccgcaggccg ttcctgcgca tcgcctcgac gtgctcggcc
      901 cagcggtcga ttgcccagac ctcgtgcccg gcggacgcga acaggcccgc gtacaccgag
      961 cccatcgccc cgcagccgac gatcgcgatc ttcatgaagt taaatgaggt ctgtccccat
     1021 ttacgcgacc tcgaacaggc ccgcggcgcc catgccgccg ccgatgcaca tggtgaccac
     1081 cacgagcttt gcgccgcggc gcttgccttc gatgagcgca tggccgacga ggcgcgcgcc
     1141 gctcaccccg tagggatgcc ccacggcgat ggcgccgccg ttcacgttga gccggtcgtc
     1201 ggggatgccg agcttgtcgc ggcagtagat gacctgcacg gcgaacgcct cgttcagctc
     1261 ccacaggtcg atgtcggaga ccttctttcc cgccatctcg agcagcttgg gcaccgcgaa
     1321 caccgggccg atgcccatct cgttgggctc gcagccggcg accgcgaagc cgcggaacac
     1381 gccgagcggc ttcaagccgc gcttcgcggc ttccttgtcg ctcatcacca ccgcggccga
     1441 ggcgccgtcg gagaactggc tggcgttgcc ggcggcgatc acgccgccct cgatcgccgg
     1501 cttgatctgc gacacgccct cgtaggtggt gtcggggcgg atgccctcgt cgtgctcgac
     1561 ggtgacctcc ctcatggtct ccacgttggt gttcttgtcg atcaccttca tcttggcggt
     1621 gatcggcacg atctcgtcct tgaacttgcc ctcggcgcgc gccttggccg cgcgctgctg
     1681 gctgcgcgcg ccgtaacggt cctgcagctc gcgcgagatg ttgtacttct tcgacacgta
     1741 ctccgcggtc tgcagcatcg gccagtagat ctcgggcttg tggcggtgga tccattcgtc
     1801 ctgggcgtgg aacttgttgg cctcgttctg caccagcgag atcgattcga cgccgccgcc
     1861 gacgaagacg gtgccttcgc cggccagcac gcgctgcgcc gcgagcgcga tggtctgcag
     1921 tcccgaggag cagaagcggt tcaccgtcat gccgggcacc gtcaccgggc agccggcgcg
     1981 aatcgcgatc tggcgcgcga tgttgccgcc ggtggcgcct tcgggcgtgg cgcagccgat
     2041 gatcacgtcc tccacctcgt cggcgtccac tttcgcgcgc tcgagcgcgg cgtgcaccac
     2101 gtgcccgccc atcttggcgc cgtgggtcat gttgagcgcg ccgcgccagc tcttgcacag
     2161 cggcgtgcgc gccgtcgata cgatcactgc ctcggtcatg tcgaagcctc ctatccgtta
     2221 aagcgcttgc cttccttggc aagcttcacg agcagcggcg cgagttgcca ctgctcgccg
     2281 tggtagccct tctggaacct ctcgatcgcg accaccacct tgtccagccc gacgcagtcg
     2341 gcgtagaaca tcggcccgcc ccggtgcggc gggaagccgt agcccgcgag gtacaccatg
     2401 tcgatgtcgc tagcccgcag cgcgatgccc tcctcgagaa tgcgcgcgcc cgtgttgacg
     2461 agcgcgtaga tgcagcgctc gacgatctcc tggtcggaga tggcgcgcgt ctgcaggccg
     2521 tgctccttgc ggtacttgtc gagcagcgcg tcgatctcgg gatcgggcag cgccttgcgg
     2581 ttgcccggct cgtagcggta aaagcccttg ccggtcttct gcccgaagcg cccgagctcg
     2641 cacaccttgt cggcgaactt ggagtacgcc atttccggcc gctcgtagta gcggcgcttg
     2701 cggatgtccc agccgatgtc gttgccggcg aggtcggaca tggtgaacgg gcccatcgcc
     2761 atgccccact tctgcagcgc cgcgtcgacc tgctgcgggc tcgcgccctc gtcgagcagc
     2821 agccccgcct gctgcgagta gcgctcgacc atgcggttgc cgatgaagcc gtcgcacacg
     2881 ccggagacga tcggcacctt cttcagcttc ttgccgagct tcatcgtggt ggcgagcacg
     2941 tccttcgcgg tcttctcgcc gcgcaccact tccagcaggc gcatgacgtt ggccggtgaa
     3001 aaaaagtgcg tgccgatcac gtcctgcggc cgcttggtca cgtcggcgat cttgttgacg
     3061 tcgagcgtgg aggtgttggt ggcgaggatc gcgccgggct tcatcagggc atcgagcttc
     3121 ttgaacatgt cctgcttgac gtccatcctc tcgaacaccg cctcgatgac gatgtcggcg
     3181 ggcttgagcg cgttcagatc cgtgctcggc tggatcagcg cgtagcgctt ttccatctcc
     3241 tcgggcttga gtctgccgcg cttcaccgtg ccctcgtagt tgtcgcggat cttcttcatg
     3301 cccttgtcga gcgcctcctg cgacacgtcg gcgatcacca ccgggatgcc ggcgttggcg
     3361 aagctcatgg cgatgccgcc gcccatggtg ccggcgccga ccaccgccgc cttcctgatc
     3421 tcgcgcgtcg gcgtgctctc gggaacgtcg gggatcttgg aggtctggcg ctcggcgaag
     3481 aacatgtggc gcagcgcctt cgattcaacg gtgttgacga ggtactcgaa gcgctcgcgc
     3541 tcgaccttgc ggccctcgtc gaagggcagc gtcaccgcgg cgtggatgca cttggcgatc
     3601 tcggcgggcg ccgggttgcc gccggcgctc ttcgccgcct gctcgggcac ggcctcgagc
     3661 agcgccttct ggtcgccctc caccttgggc ggcatgtcgc ggatgcgccg cggccccttg
     3721 ccggcggcca ccagcgtctg cgcgtaggcg atgccggcct cgagcaggtc gccgctcacg
     3781 atctcgtcga tgaggccgag ctccttgcct ttgggcgccg gaatcgggtc gccgagaatc
     3841 atcatgcgcg ccgcctcgtg caccgggatg atgcggggca ggcgctgcgt gccgcccgag
     3901 cccggcagga tgccgagctt cacttccggc aggccgagca gcgacttctc cagcgcgacg
     3961 cggtagtggc agccgagcgc gagctccagc ccgccgccca gcgcgaagcc gccgatggcg
     4021 gccaccaggg gcttttgcat cgagtcctgg atgtcgttca cctccgggag aatcggcttg
     4081 gtcatcggct tgccgaactc gcggatgtcg gcgccgcccg agaacgcctt cgcggagccg
     4141 atcagcacca ccgccttgat gtcgggatcg gaatcggctt tttcgaggcc ggcggcgatg
     4201 cccgcgcgca gcgcgttgcc gaggccgttc acgggcgggt tgttgagggt gatgacggcg
     4261 atgccgtcgc gcacggaata ggctgccacg tcgctcatgt cgcgcattct cctggggtcg
     4321 gccaaattct accctcccgc tcccggcggc ggatagtgct tgaccggctt cggcggcgca
     4381 cagtaagttt tgattcgagg agggcagagg catgcatggc ctgatgatgg acgtcccgct
     4441 gctgatcacc gacctgatcc gcttcgcgga ccgcaatcac ggcgataccg agatcgtgtc
     4501 gaagaccgtg gagggcgagc tgcaccgcac cacctaccgc gacgcgcacg cgcgcgcgcg
     4561 gcgcctcgcc aaggtgctgc aggcgctcgg cgtgaaggcg agcgatcgcg tcggcaccct
     4621 ggcctggaac ggctaccggc actacgagct ctactacgcc atctcgggca tcggcgccgt
     4681 catcaacacc atcaatccgc gcctgtttcc cgaccagatc gtctacatct cgaaccacgc
     4741 cgagaaccag gcgctgttct tcgacctcac cttcgtgccg ctggtggaga agctcgcgcc
     4801 gcagctgaag acggtgaagc acttcgtgct gatgaccgac cgcgcgcaca tgccggccaa
     4861 gagcccgatc ccgaatctgc tgtgctacga ggagctgatg gccaagcagg acgagcgctt
     4921 cgagtggcct tccttcgacg agcgcacggc ggcggcgctg tgctacacct caggtaccac
     4981 gggcaacccc aagggggtgc tctacggcca ccgctcgacg gtgctgcacg cctacggcgc
     5041 cgcgctgccc gacgcgctca acctctcggc cggcgactcg gtgctgccgg tggtgccgat
     5101 gttccacgcc aatgcctgga gcctgccgta tgcctgcacc atggtcggcg cgaagatggt
     5161 gttcccggga ccgcacctcg acggcaagag cgtgtacgac ctgatcgagc aggagaaagt
     5221 gacgctctcg gccggcgtgc cgacggtgtg gctgatgctg ctgcagcacc tcacggccca
     5281 caaggcgaaa ttttcgacgc tcaagcgcac cgtgatcggc ggctcggcct gcccgccggc
     5341 catgatccgc gccttccagg acgactacgg cgtgaccgtg ttccacgcct ggggcatgac
     5401 cgagatgagc ccgctcggca cggtgggctc gctgaaggcg aagcaggcgg cgctgccgaa
     5461 ggacgcgcgc tacgccgtgc aggcgaagca gggccgcggc atcttcggcg tcgagatgaa
     5521 gatcgtcgac gaggagggca aggaactgcc ctgggacggc aagcgcttcg gcaacctgct
     5581 ggtgaagggt ccctggatcg cctcgggcta catgaagggc gaaggcggcg atccgctgaa
     5641 ggacggctgg tttccgaccg gcgacgtcgc caccatcgac cccgagggct acatgcagat
     5701 caccgatcgc tccaaggacg tgatcaagtc gggcggcgag tggatcagct cgatcgacct
     5761 ggagaacatc gcggtggcgc accccgcggt gcaggaggcc gcggtgatcg gcatccgcca
     5821 tcccaagtgg gacgagcgcc cgatcgtggt ggcggtgagg aaatccggcc agcaggtgac
     5881 gaaagaggac ctgctcaagt tctacgaggg caagatcgcc aagtggtgga tgcccgacga
     5941 cgtggtgttc gtcgacgagc tgccgcacac ggcgaccggc aagctgtcca agctcacgct
     6001 gcgggagaag ttcaaggact accggctgcc gggcgcatga gccacgtcct cgtcgagacg
     6061 cgcgagcgcg tcacccgcat cgagctgaat cgcccggaga agaagaacgc gctcagcgcc
     6121 gagatgtacg cggcgatggc aaaggcgctc gccgcggccg aggccgacgc gcaggtgcgc
     6181 gcggtgctga tccacggcca ggccgactgc tttacctcgg gcaacgacgt caaggacttc
     6241 ctcgagctgc cgcgcgggcc gggcgactcg cccgccacgg gattcctgcg cgccatctcc
     6301 gcggcaaaga agccgctggt ggccgcggtc ggtggagcgg cgatcggcat cggcacgacg
     6361 atgctgctgc actgcgacct ggtgtacgcg gcgcccaatg cgcggctgca gatgccgttc
     6421 gtgccgctcg gcctgctgcc ggaagccgcc tcgagcctgc tgctgccggc gatggcgggc
     6481 taccggcgcg cagccgagat gctgctcctc ggcgggccgt tcggcgcgga ccaggcgctc
     6541 gcggccggca tcgtcaccga gatcgtgccg cagaccgagc tcctcgagcg cgcccgccag
     6601 gcggcgctgg cgctcgcggc gctgccgccc gcctcggtgc gcatgacgaa ggagctgatg
     6661 aagcgcggcc tggccgcagc cgtgcaggag cgcatgaccg aggagctggg cctgttcggc
     6721 gagcggctgc gctcgcccga ggcgaaagag gcgctcagcg ccttcctgga gaagcgcagg
     6781 cccgacttct cgcgcttctc gtgagcctcg ttcacgagac ggttgcggcg aacggcctga
     6841 agttccacgt cgcgcgcgcg ggcgaggggc cgctgatgct cttcctgcac ggctttcccg
     6901 aatactgggc gatgtggcgg ccgctgctcg agcatttcgg cgcgcgcggc ttctgcgccg
     6961 cggcgcccga cctgcgcggc tacaacctgt cggacaagcc cgccgcggtc gaggcttacc
     7021 gcgccaagca cctgatggcc gacgtcctcg cgctcgccgc gcactatacg aacgaaaagt
     7081 tcgtgctggt cgcccacgat tggggcggcg cggtcgcctg gggcgtggcg atcgcgcacc
     7141 ccgagcggct cgcgaagctg gcgatcgtca atgcgccgca tccgtacctg ttctggcgcg
     7201 agctctgcac cagccccgcg cagcagaagg cgagcgagta catgaacttc tttcgtctcg
     7261 ccaaggccga gcgcgtgctc gccgagaacg gctacgcgcg cctgctcggc gccttttccg
     7321 acctggacga ggcggcgcgc acggcgctcg tcgaagcctg gtcgcagccc ggcgcgctca
     7381 ccggcgggct caactattac cgcgcctcgc cgatgtatcc gccgagcgcg gacgacccgg
     7441 gcgcgaagaa gctgcagctc aagcccgagg acttcgtggt cagggtgccg acgctcgtcg
     7501 tctggggcga gcgggacacg gcgctgctcc ccggctgcat tgacgggctc gaggccgtag
     7561 tccccgacct gaagctggtg cgggtgcccg aggcctcgca ctggatcgcg cgcgagcgaa
     7621 ccgacctcgt catccgcgag atcggggcgt ttacaatcgg ccgatgaaga cttcgctcag
     7681 gatccgcgac ctcgaatcgc gcgtcggcca ggaaatcgcg cagtcgccct gggtggagat
     7741 gccgcaggag cgcatcgacc tcttcgcgaa ggcgaccgag gactaccagt ggatccacgt
     7801 cgacccggag cgggcgaaag cctcgccgtt cggcaccacc atcgcgcacg gcttcctcac
     7861 gctctccatg ctgccgaagc tgatcgagtc gaccttcgag ttttccgacc gcaagatggg
     7921 cgtcaactac ggcctgaaca aggtgcgctt cacttcgccg gtgcccgcgg gcgcgaagat
     7981 ccgcggccgc ttcacgctgg cgaaatacga gaagctggac ggcggcgtgc agaccacctg
     8041 gagcgtgacg gtggagcgcg agggcggcga gaagcccgcg atggtggccg agaccatcag
     8101 ccggcactac ttctgacatg acgcagcgct ggaagcacaa gcccccgaac tccaactggg
     8161 gcgagttcgg ccccgacgac cagcgcgggc gcatgaacta cgtgacgcgc gagaaggtgc
     8221 tgcagggcgt cgccgaggtg aaggagggga tcgcgttctg cctgtcgctg ccgctggact
     8281 acccgggcgg caacgcgctc aacccgcgcc ggcgcccgcc gcgcctcgcg ccgacgatcc
     8341 gcgacggcaa gcaggcgttc tgcctgccgc tcgccgagtt cgtcggcgac gtcaccgacg
     8401 tggtgtgcga cgaccaggtg ctgctcgcgc tgcagtactc gtcgcagtgg gactcgttcg
     8461 cgcacgtcgg ggcgaagttc gacgccgacg gcgacggcaa ggcggagatc gtgttctaca
     8521 acggctttcg cgccggcgag cacgtgaagc ccgggccgga gaagcccgac gccgagccct
     8581 gggcgaagtt cgagggcgcg cgcgccgagg cgctcggcat ccagaacctg gccgagcacg
     8641 gcgcgcaggg ccgcggcgtg ctggtcgacc tgcacgcgca cttcggccgc gaaaggaagg
     8701 cgatcggcta cgacgcgctg atgcgcgtca tggaaaagga caaggtggtg gtggagaagg
     8761 gcgacttcgt gtgcttctac accggtttcg ccgacgtgct gctggagctg aagaagaacc
     8821 ccgacccgca gctcatccac aacacctgca ccgggctcga ggggcgcgac gacaggctcc
     8881 tgcagtggat caccgactcg ggctgcgcgg cgctgatcgc cgacaactac gccgtggaga
     8941 tcatcccggc gagcccgttc aagccgctgc cgcacgcgtt gatgccgctg cacgagcact
     9001 gcctgttcaa gaacggcatc cacctgggcg agatctggta tctcaccgag ctggcgcgct
     9061 ggctgcggga gcaccggcgc aaccgcttcc tgctcaccgc gccgccgctg cggctgcccg
     9121 gcgccgtggg ctccccggcg cccgcggtag ctaccgtata aggaggaccc gatggccgac
     9181 aacgaccggc gcgacctcgt cgcgcaaatg aagaaccgtg cgctcatcta caaggaaacc
     9241 tacgacgtgc tcgccgagga gctcggcgcg gcgaaggccg aggcgctgct gacgcgcgcc
     9301 atctaccagc gcgggcgcgc aatgggcaag tcgttcgcca agtacgcgcc cgccgatctg
     9361 aacgggctgc gcgaagcctt cctcgccagc gtgccgggcg gcgccgacat cttccagccc
     9421 gaggtgctga agaaggaggc tgcgacgctc gagatccagc tgcacggctg cccgctgaag
     9481 gacgcctggc gcgaagccgg attgccggaa gacaaggtgg ccacgctgtg ccgcatcgcc
     9541 ggcgccgtgg acaagggcct gttcgaaggc gccgggttca agatcgagaa ccgcacctgg
     9601 acgcccggcg cgaaaggctg ctgcttcctg cgcatcctgc gtggctgagc actgggacta
     9661 tatcgtcgtc ggcgccggca cggcgggctg cgtgctggcg aatcgcctca ccgaggatgg
     9721 ccggcaccgc gtgctgctgc tggaggccgg accgcgcgac accaacccct ggatccacgt
     9781 gccgcttggc tacggcaagc tcttcacgcg caccgacgtc aactgggcct acgaatccga
     9841 gcccgagccg acgctgaacg gccggcgcat cttcaccccg cgcggcaagg tgctgggcgg
     9901 ctcctcctcg atcaacggcc tcgtgtacat ccgcggccag cgcgaggact tcgacggctg
     9961 gggccttgcc ggctggcgct tcgacgagct gctgccgtac ttcaagaagt ccgaggacca
    10021 ggggcgcggc gccgacgcct ggcacggcgt gggcgggcct atcggcgtca acgacctgcc
    10081 ggaccggcac gagctgtgcg acgcgttcat cgcctcggcc gaggcgctcg gcgtgccgcg
    10141 caacgacgac ttcaacggcg cgcgccagga gggcgcgggc tactaccagg cgacgaccag
    10201 gaacggccgg cgctcgagcg ccgccaccgg gtacctgcgc ccggcgcgaa agcgcgccaa
    10261 cctgcgcgtc gagaccgaag cgctcgccac gcgcgtgctg ttcgacggca cgcgcgccgt
    10321 gggcgtggag tactcgcagg ggggcgccac gcgtcaggcg cacgtggcgc gcgaggtgat
    10381 cctcgcgggc ggctcgatca attcgccgca gctgctgcag ctctcgggcg tgggcccgcg
    10441 cgcgctgctc gagcgccacg gcatccggcc gctgcacgac gcccccggcg tcggcgagga
    10501 cctgcaggac cacttctacg tgcgcacctt ctggcgctgc acgaagccga tcacgctgaa
    10561 cgacgacatg gcgagctggt ggcgccagct cggcatcggc ctgcgctacg tcctgcagcg
    10621 gcgcgggccg ctcacggtca gtgccggcta cgctgcggca ttcgtgcgta cgcggcccga
    10681 gctcaagcgc ccggatgggc agatctactt catcaacttc tccacggcca agcgcggcgg
    10741 cgtgctccac ccgttcccgg gcttcaccct gtcgctgtcg cagctgcagg ccgagtcgcg
    10801 cggctgggtg cggatccgct cggccgaccc gaaggcgccg ccggcgatcc actacaacta
    10861 cctgtccacg gaaaaagagc agcgcacgat ggtcgacggc ctgaagctgg tgcgccgcct
    10921 ggcgcagacg ccgccgctcg cgggctacgt ggcggaggaa ttccagccgg gaccgaaggt
    10981 gcaaagcgac gccgactggc tcgcctatgg ccgcgacaat ggcgatacgg tgttccaccc
    11041 cacctcgacc tgccgcatgg gcgaggacgc gcgcgcggtg gtggatgcgc gcctcagggt
    11101 gcgcgggctg cagtccctgc gggtgatcga cgcctcggtg atgccggcgg tggtctcggg
    11161 gaacaccaac gcggcggttt tcgccattgc ggagaagggc gccgatctgg tgcgcgcgga
    11221 cgcgaatcgc gcttgacacg gcgggccggt ctgcgaaaga ctggcccgct caa
//
LOCUS       DBNHKE010000162         1870 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_980270, whole genome shotgun sequence.
ACCESSION   DBNHKE010000162 DBNHKE010000000
VERSION     DBNHKE010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_980270"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..488)
                     /locus_tag="AC16O9_11000"
     CDS             complement(180..488)
                     /locus_tag="AC16O9_11000"
                     /inference="COORDINATES: protein motif:HMM:NF023171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HIR6517183.1"
                     /translation="MWELAVLALALAAAWLWIDSLRAREHAVAAGRAACKRYGVQLLD
                     ETVAFARLRLARDDAGQLRLRRTYTFEFSDTGDNRRHGAIVMLGARLEDLQLEPYPLQ
                     "
     gene            549..1541
                     /locus_tag="AC16O9_11005"
     CDS             549..1541
                     /locus_tag="AC16O9_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6517184.1"
                     /translation="MLRDPPTAADLAHAPPREVFAAIAAALDARVVGQQALTQRLLIA
                     LLCDGHVLVEGVPGLAKTTAVKTLAELLEADFHRIQFTPDLLPADLTGTEVFRAETGT
                     FEFRPGPVFRSLVLADEINRAPAKVQSALLEAMEERQVSVGRASFPLPELFMVMATQN
                     PIEQDGTYPLPEAQLDRFLLHVTVGYPPPEAEHAIMQLARNGPGRAPAQAIASSAQVF
                     AARREALALHMAPSLERYIVELVLATREPGAYDAELAPLIRLGASPRASIGLDRAARA
                     HAWLAGRDYVSPADIQAIAPDVLRHRLLLSYQSRADGVGADAIVARLIAQVPVP"
     gene            1538..>1870
                     /locus_tag="AC16O9_11010"
     CDS             1538..>1870
                     /locus_tag="AC16O9_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6517185.1"
                     /translation="MTSAGTPRHVPRGVRPALDQLAGLRRYGAQMRRLLSLDAARRDG
                     PRAPAARSQGMDYADSRPYTPGDDVRRIDWRVTARTGRAHTKLFHLERGNDVYCIVDQ
                     RAAMRFGTR"
BASE COUNT          228 a          718 c          670 g          254 t
ORIGIN      
        1 acgatcttgc cggtggcgtc gatctcggcg tcgccgcttg cgcccgtcag ctcgccgatc
       61 gccgcgatgc gcccgtccgc gacggcgacg tcggcggtgg tgcgcggcgc gcgcgtgccg
      121 tcgatcacgg tgccgccgcg gatcagcagg tcgtagcggg tgcccatagc gcctgcattc
      181 tattgcagcg gatacggctc gagctgcaga tcctcgagcc gcgcgccgag catcacgatc
      241 gcgccgtggc gccggttatc gccggtgtcg gagaactcga acgtgtaggt gcgccgcagc
      301 cgcagctgcc ccgcgtcgtc gcgcgcgagg cgcaggcgcg cgaacgcgac ggtttcgtcg
      361 agcagctgca cgccatagcg cttgcatgcg gcgcgcccgg cggcgaccgc atgctcgcgc
      421 gcgcgcaggc tgtcgatcca cagccaggcg gcggcgagcg cgagcgctag gacggccagt
      481 tcccacatcg gtcgggaagt gtagcccggg ccttgttcgc gccccgtgcc tcaaggcaca
      541 ctggcgccgt gctgcgcgac ccccccaccg ccgccgacct ggcgcacgcc ccgccgcgcg
      601 aggtgttcgc tgccatcgcg gccgcgctcg atgcgcgcgt cgtcggccag caggcgctca
      661 cgcagcgcct gctgatcgcg ctgctgtgcg acgggcacgt gctggtcgag ggcgtgccgg
      721 gcctggccaa gaccaccgcg gtgaagacgc tggccgagct cctggaagcc gacttccacc
      781 gcatccagtt cacgcccgac ctgctgcccg ccgacctcac cggcaccgag gtgttccgcg
      841 ccgagactgg caccttcgag ttccgccccg ggccggtgtt ccgcagcctc gtcctcgccg
      901 acgagatcaa ccgcgcgccc gccaaggtgc agtcggcgct gctggaagcg atggaggagc
      961 gccaggtgag cgtcgggcgc gcgagctttc cgctgcccga gctcttcatg gtgatggcga
     1021 cgcagaaccc gatcgagcag gacggcacct atccgctgcc cgaggcgcag ctcgaccggt
     1081 tcctgctgca cgtgacggtg ggctatccgc cgccggaggc cgagcacgcc atcatgcagc
     1141 ttgcgcgcaa cggcccgggg cgtgcgccgg cgcaggcgat cgcgtcgtcg gcgcaggtgt
     1201 tcgccgcgcg ccgcgaggcg ctcgcgctgc acatggcgcc ctcgctcgag cgctacatcg
     1261 tggagctggt gctggcgacg cgcgagccgg gcgcctacga cgccgagctc gcgccgctca
     1321 tccgcctcgg cgcgagcccg cgcgcctcga tcggcctgga tcgcgcggcg cgcgcgcacg
     1381 cctggctcgc cgggcgcgac tacgtctcgc ccgccgacat ccaggcgatc gcgcccgacg
     1441 tgctgcgcca tcgcctcctg ctgtcgtacc agtcgcgcgc cgacggcgtc ggcgccgacg
     1501 cgatcgtcgc gcgcctcatc gcgcaggtgc cggtgccgtg acgagcgccg ggacgccgcg
     1561 gcacgtgccg cgcggcgtgc gcccggcgct ggatcagctc gcgggcctgc ggcgctacgg
     1621 cgcccagatg cggcgcctgc tctcgctcga cgccgcgcgc cgcgacggcc cgcgcgcgcc
     1681 cgcggcgcgc agccagggca tggactacgc cgattcgcgg ccctacacgc cgggcgatga
     1741 cgtgcggcgc atcgactggc gcgtcaccgc gcgcaccggg cgcgcgcaca ccaagctgtt
     1801 ccacctcgag cgcggcaacg atgtttactg catcgtcgac cagcgcgcgg cgatgcgctt
     1861 cggtacgcgc
//
LOCUS       DBNHKE010000163         6301 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_716552, whole genome shotgun sequence.
ACCESSION   DBNHKE010000163 DBNHKE010000000
VERSION     DBNHKE010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_716552"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="AC16O9_11015"
     CDS             <1..226
                     /locus_tag="AC16O9_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIR6517186.1"
                     /translation="TDKGIVLYRRLLVDAIQKAAKGERTLMRPDAAQAAALTGPPAVD
                     GVGPTGRWDEYCREADAARRNRAPWSAKAA"
     gene            223..1593
                     /locus_tag="AC16O9_11020"
     CDS             223..1593
                     /locus_tag="AC16O9_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6517187.1"
                     /translation="MNDLLKAAAGLEAVRFAFADQHGLLRSRTFAAAELAAVLKSGVG
                     FPSSLLAKDTSNKTVFPVFTAGAGFGMPELEGAADAVMVPDAQTFRVLPWVPKTGWVL
                     CDLKFPNGRPVPFDTRGLLKKALASLADAGYGYRAGLEIEFHIFRLVDPRLRPEDAGQ
                     PGSPPEVALLTSGYQLLSETRHDQLEPALDALRAGLEGLELPLRSFECEFGPSQAEVT
                     LGVQDGLAAADAMVLFRSAAKQILRRHGYHATFMCRPRLPNVMSSGWHLHQSLTDRSG
                     ANAFTAEKDFLSATGKGFLAGLLEHAAASAAFATPTLNGYKRYRPYSLAPERVVWGRD
                     NRGAMLRVLGGPGDPATRIENRIGEPAANPYLYFASQIVCGMDGVQRKLDPGAPEHTP
                     YESKAALLPRSLEEALAALKADGVLEESFGAGFVDYYCRIKQAEIARFNAEVSDWEQR
                     EYFDLF"
     gene            complement(1650..2087)
                     /locus_tag="AC16O9_11025"
     CDS             complement(1650..2087)
                     /locus_tag="AC16O9_11025"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6517188.1"
                     /translation="MARSRDGGCVCGAVRYRMKAAPQVGLVCHCTWCQKRSGSAFSFN
                     AYFRDEDVEFLQGKLSRYEHRSDETGRWLRTEFCPVCGTPITHTTELRPGLRAIAAGT
                     MDDPDSFTIGRHIWSRSARPWVAIPEGVEVYEKGSQDATPIRR"
     gene            2188..3630
                     /locus_tag="AC16O9_11030"
     CDS             2188..3630
                     /locus_tag="AC16O9_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024985.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HIR6517189.1"
                     /translation="MKSFRLQAAVAAAFCSALTAHAAVTERPSTLADQQSVAVTIYNE
                     NLALIKDVRKIALDSGPNRLALREVSAQMRPETALLRSLSHAGALRLIEQNFDFDLLT
                     PAKLLEKYVGRDVRIVRVHPQTGAESIETATVLAANQGVVLKIGDRIETGLPGRIVYD
                     GVPANLRDKPTLVSELASTRAGSQTVELSYLSGGLAWKADYVAELNAKDDALDLNGWV
                     TLTNRSGTSYPEARLQLVAGDVNRVRDELVMAAKVGRMRADEARAPASMAQETLFEYH
                     LYTLQRPTTIQNNQTKQVALLSGTSVPVQKELVLQGNDYYYRSSVGHLGQKMKVGVFV
                     QFENREASRLGLPMPKGVVRVYKKDSAGNAQFVGEDRIDHTPKNETVRLKLGEAFDVT
                     ADKKQTDFKRRDTYSPWSYVHESAYEIVLKNAKSEAVTVVVREPVPGDWKMLEETQRH
                     KKAAAGTAEWNIRVPAEGSTTLRYRVLVRY"
     gene            3634..4341
                     /locus_tag="AC16O9_11035"
     CDS             3634..4341
                     /locus_tag="AC16O9_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6517190.1"
                     /translation="MKDLTPTGKLRVGVNLSNFLLVQSNPDGTIRGIVPDLAQELAKR
                     LGVAAELRKYEKVGDVADGAKKGEWDVAFIGAEPQRAAEIDFTAPYVEIEATYLVPAG
                     SPIRTLADVDRKGVRIAVAERSAYHLWLERNIKHATLVTAAGIEASYQVFVKDKLEVL
                     AGLKPRLMEDVAKLPGATILEGRFTAVQQAIGTPKGRPEGAAYLRKFAEDIKSSGLVA
                     QLIGRHKIRGLSVAAPG"
     gene            4349..5026
                     /locus_tag="AC16O9_11040"
     CDS             4349..5026
                     /locus_tag="AC16O9_11040"
                     /EC_number="4.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6517191.1"
                     /translation="MSGLRLTRETILDGTLHASVRALLGPNTRFATDAERSAQVQAIL
                     ARAPRPGRIWVFAFGSLMWNPAFHFVERRTARIHGFHRQFCLWSRAGRGSPERPGLML
                     SLESGGSCTGVAYRLERRAAATELDVIWRREMFTMSYRPVWTLARTPKGPEPAIAFAA
                     NRAHERYVPGLEDEVIARYLATGAGPMGRCCDYLFDTVAHLRQLGIRDRRLEGLEARV
                     RAHSATA"
     gene            5028..5726
                     /locus_tag="AC16O9_11045"
     CDS             5028..5726
                     /locus_tag="AC16O9_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6901 family protein"
                     /protein_id="HIR6517192.1"
                     /translation="MAKLVYRFSFPDGRTESLDAGAGAPQDALPHWTELGFHQCANCP
                     LSSADTKRCPMAVRLVPLVALFEKVRSYDDVAAQVESDERTVSKRTSVQKVLRSLMGL
                     LSASSDCPHIEFLKPMAHYHLPFSSREETVYRVVSTYLLAQHFLRHQGKPADAGLEGL
                     KAHYRALQQVNQGMAARLGAIRDEQGDSSVNALVLLDLFAHSLPDSIDADLEELMPAF
                     QEFLKIDYDRADNG"
     gene            5719..>6301
                     /locus_tag="AC16O9_11050"
     CDS             5719..>6301
                     /locus_tag="AC16O9_11050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6517193.1"
                     /translation="MAEADAVTLTKSLLGYDTVNPPGREADCARHAARLLADWGYDVK
                     THDFEPGRTSVVARAGGNGGRAPLCLTGHLDVVPLGTRAWSRDPFKGETDGDKLYGRG
                     TSDMKSGVAAIMLAAKRFAKKLQGTPGVVLVLTASEENGCIGSRHLAALPKLMGKAGA
                     LVVGEPTSNHPYVGHKGSLKFHADFRGVSAHGSM"
BASE COUNT         1029 a         2227 c         2134 g          911 t
ORIGIN      
        1 gacggacaag ggcatcgtgc tctaccggcg gctgctggtc gacgcgatcc agaaggcggc
       61 gaagggcgag cgcacgctga tgaggcccga tgcggcgcag gccgccgcgc tcaccggacc
      121 gcccgcggtg gacggcgtcg ggccgaccgg gcgctgggac gagtactgcc gtgaagccga
      181 cgctgctcgg cgcaaccgcg cgccgtggtc ggcgaaggcg gcgtgaacga cctgctgaag
      241 gcggccgccg ggctggaggc ggtacgcttc gcgttcgccg accagcacgg cttgctgcgc
      301 agccggacgt tcgccgccgc cgagctcgcg gcagtattga agagcggcgt cggcttcccg
      361 agctcgctgc tcgcaaagga cacctccaac aaaaccgtgt tcccggtgtt cactgcggga
      421 gcgggcttcg gcatgcccga gctcgaaggc gcggccgacg cggtgatggt tccggatgcg
      481 cagacgtttc gcgtgctgcc ctgggtgccg aagaccggct gggtgctctg cgacttgaag
      541 tttccgaacg gcaggccggt gccgttcgat acgcgcgggc tgctgaagaa ggcgctcgcg
      601 agccttgccg acgcgggcta cggctaccgc gccggcctgg agatcgagtt ccacatcttc
      661 cgcctcgtcg atccgcgcct gcgccccgag gacgccggcc agcccggctc gccgcccgag
      721 gtggcgctgc tgacctccgg ctaccagctg ctttcggaaa cgcgccacga ccagctcgag
      781 cccgcgctgg atgcgctgcg cgccgggctc gaaggcctcg agctgccgct gcgcagcttc
      841 gagtgcgagt tcggcccgag ccaggccgag gtcacgctcg gcgtgcagga cgggcttgcc
      901 gcggccgatg cgatggtgct ctttcgcagc gccgcgaaac agatcctgcg gcggcatggc
      961 taccacgcca ccttcatgtg ccggccgcgg ctgccgaacg tgatgtcctc gggctggcac
     1021 ctgcaccagt cgctcaccga tcgctcgggg gcgaacgcgt tcacggcgga gaaggacttc
     1081 ctttccgcga cgggcaaagg gtttctcgcc ggactgctcg agcatgctgc ggcgtcggcg
     1141 gccttcgcca cccccacgct gaacggctac aagcgctacc ggccctactc cctcgcgcct
     1201 gagcgggtgg tgtggggccg cgacaaccgc ggcgcgatgc tgcgggtgct gggcggcccc
     1261 ggcgatcccg cgacgcgcat cgagaaccgc atcggcgagc cggcggcgaa tccgtacctg
     1321 tactttgctt ctcagatcgt gtgcggcatg gacggcgtgc agcgcaagct cgatcccggc
     1381 gcgcccgagc atacgccgta cgagtcgaag gctgcgctgc tgccgcgctc gctggaggag
     1441 gctttagcgg cgctgaaggc cgatggcgtg ctcgaggaga gcttcggcgc cgggttcgtg
     1501 gactactact gccgcatcaa gcaggccgag atcgcgcgct tcaacgccga ggtctcggac
     1561 tgggagcagc gggagtactt cgacctgttc taaatgggga cagacctcat ttagccggct
     1621 gtataaatga ggtctgtccc catttacggc taccgccgta tcggcgtcgc gtcctgcgag
     1681 cccttctcgt acacctcgac cccctccgga atcgccaccc acggccgcgc cgagcgcgac
     1741 cagatgtgcc ggccgatcgt gaagctgtcc ggatcgtcca tcgtccccgc cgcgatggcg
     1801 cgcaagccgg ggcgcagctc ggtggtgtgg gtgatcggcg taccgcacac gggacagaac
     1861 tcggtgcgca gccagcgccc ggtctcgtcc gagcggtgct cgtaccgaga cagcttgccc
     1921 tgcaggaact ctacatcttc gtccctgaag tacgcgttga aggaaaacgc gctccccgag
     1981 cgcttctggc accaggtgca gtggcacacc agccccacct gcggcgcggc cttcatccgg
     2041 tagcgcaccg caccgcacac gcacccgccg tctctcgatc tcgccatggg aacctccggt
     2101 ggtcgctcct cattgtgcaa caacattaac cctttccgga ttcgcgtacg ttcaatagca
     2161 cgtacccaac ccgaaaggag ccccaccgtg aaatccttcc gcctccaggc cgccgtcgcg
     2221 gcggccttct gctcggcgct caccgcccac gcggcggtca ccgagcgacc gagcaccctc
     2281 gccgaccagc agtcggtcgc cgtgacgatt tataacgaga acctggcgct gatcaaggac
     2341 gtgcgcaaga tcgcgctcga tagcggcccc aaccgcctcg cgctcagaga agtcagcgcg
     2401 cagatgcgcc ctgaaacggc gctgctgcgc agcctgtcgc acgccggcgc gctgcgcctc
     2461 atcgagcaga acttcgactt cgacctgctc accccagcga agctgctgga gaagtacgtc
     2521 gggcgcgacg tgcgcatcgt gcgcgtgcac ccgcagaccg gcgccgagtc gatcgaaacc
     2581 gccacggttc tggccgccaa ccagggtgtg gtgctgaaaa tcggcgatcg catcgagacc
     2641 ggccttccgg gacgcatcgt ctacgacggc gtgccggcga acctgcgcga caagccgacg
     2701 ctcgtaagcg agctcgccag cacccgcgcc ggcagccaga cggtggagct gtcctacctc
     2761 tcgggcggcc tcgcctggaa ggcggactac gtcgccgagc tgaacgcgaa ggacgacgcc
     2821 ctggacctca acggctgggt cacgctcacc aaccgcagcg gtacctccta ccccgaggcg
     2881 cgcctgcaac tcgtcgccgg cgatgtgaac cgcgtgcgcg acgagttggt catggcagcc
     2941 aaggttgggc gcatgcgcgc cgacgaagcg cgcgccccgg cgagcatggc gcaggagacc
     3001 ctgttcgagt accacctgta cacgctgcag cggccgacca cgatccagaa caaccagacc
     3061 aagcaggtgg cgctgctcag cggcacgagc gtgccggtgc aaaaggagct ggtgctgcaa
     3121 ggcaacgact actactaccg ctcgagcgtc ggccacctcg gccagaagat gaaggtcggg
     3181 gtcttcgtgc agttcgagaa ccgcgaggcc tcgcgcctcg gcctaccgat gcccaagggc
     3241 gtggtgcgcg tgtacaagaa ggactccgcc ggcaatgccc agttcgtggg cgaggaccgc
     3301 atcgaccaca cgccgaaaaa cgagaccgtg cgcctgaagc tgggcgaggc gttcgacgtc
     3361 acggccgaca agaagcagac cgacttcaag cgccgcgaca cctacagccc ctggagctac
     3421 gtgcacgaga gcgcctacga gatcgtgctg aagaacgcca agagcgaggc ggtcaccgtc
     3481 gtggtgcgcg agccggtgcc gggggactgg aagatgctcg aggagaccca gcgacacaag
     3541 aaggccgctg cggggaccgc agagtggaat attcgcgtcc ccgccgaggg cagcaccacc
     3601 ctgcgctacc gggtgctggt acgctactga cctatgaaag acctcacccc caccggcaag
     3661 ctgcgcgtag gcgtcaatct cagcaacttc ctcctcgtgc agagcaatcc cgacggcacg
     3721 atccgcggca tcgtccccga cctggcgcag gagctcgcaa agcggctcgg cgtcgcggcc
     3781 gagctgcgaa agtacgagaa ggtcggcgac gtcgccgacg gggcgaagaa gggcgagtgg
     3841 gacgtcgcct tcatcggcgc cgagccgcag cgcgccgccg agatcgactt caccgccccc
     3901 tacgtcgaga tcgaggccac ctacctcgtg cccgcgggct cgcccatccg gacgctcgcc
     3961 gacgtggacc gcaagggcgt gcgcatcgcg gtcgccgagc gcagcgccta ccacctctgg
     4021 ctcgagcgca acatcaagca cgccacgctg gtgaccgccg cggggatcga ggcgtcctac
     4081 caggtgttcg tcaaggacaa gctcgaggtg ctcgcgggcc tgaagccgcg gctgatggag
     4141 gacgtggcca agctccccgg cgcgacgatc ctcgagggcc gcttcaccgc cgtgcagcag
     4201 gcgatcggca cgcccaaggg ccggcccgag ggcgcggcct acctgcgcaa gttcgccgag
     4261 gacatcaagt cctccgggct ggtggcgcag ctcatcgggc gccacaagat ccgcggcctg
     4321 tcggtggcgg cgccgggcta gcctcggcat gagcggcctc cggctcacgc gcgagaccat
     4381 cctcgacggc acgctgcacg cctcggtgcg cgccctgctc gggccgaaca cgcgctttgc
     4441 caccgacgcc gagagaagcg cgcaggtaca ggcgatcctc gcccgggcgc cacgcccggg
     4501 gcgcatctgg gtgttcgcgt tcggctcgct gatgtggaat ccggcgttcc atttcgtcga
     4561 gcgccgcacg gcgcgcatcc acggctttca tcgccagttc tgcctgtggt cgcgcgccgg
     4621 ccgcggcagc cccgagcgcc ccgggctgat gctgtcgctg gaatcgggcg gcagctgcac
     4681 cggggtcgcc taccgcctgg agcggcgcgc ggcggcgacc gagctcgacg tcatctggcg
     4741 gcgcgagatg ttcaccatgt cctaccgccc cgtgtggacc ctggcgcgca cgcccaaggg
     4801 ccccgagccg gcgatcgcct tcgccgccaa ccgcgcgcac gagcgctacg tgccggggct
     4861 cgaggacgag gtcatcgcgc gttacctcgc caccggcgcc ggccccatgg gccgctgctg
     4921 cgactacctg ttcgacaccg tggcgcacct gcgccagctc ggcatccgcg accgccggct
     4981 ggaagggctc gaggcccggg tgcgcgcgca cagcgcgacg gcctagcgtg gcgaagctcg
     5041 tctaccgctt cagcttcccc gacggccgca ccgagtcgct ggacgcgggc gccggcgcgc
     5101 cgcaggacgc actgccgcac tggaccgagc tcggcttcca tcagtgcgcc aactgcccgc
     5161 tgtcgagcgc cgacacgaag cgctgcccga tggcggtgcg cctggtgccg ctggtggcgc
     5221 tgttcgagaa ggtgcgctcg tacgacgacg tagcggcgca ggtggaatcc gatgagcgca
     5281 cggtcagcaa gcgcaccagc gtgcagaaag tgttgcgctc cctgatgggc ctgctctcgg
     5341 cgagcagcga ctgcccgcac atcgagttcc tgaagccgat ggcgcattac cacctgccgt
     5401 tctcgagccg cgaggagacc gtctaccgcg tcgtctcgac ctacctgctc gcgcagcatt
     5461 tcctgcgcca ccagggcaag cccgccgacg cggggctgga ggggctgaag gcgcactacc
     5521 gcgcgctgca gcaggtcaac cagggcatgg cggcgcggct gggcgcgatc cgcgacgagc
     5581 agggcgacag cagcgtcaac gcgctggtgc tgctcgacct gttcgcgcat tcgctgccgg
     5641 attccatcga cgccgacctc gaggagctga tgccggcgtt tcaggaattc ctcaagatcg
     5701 actacgatcg tgccgacaat ggctgaagcg gacgccgtta ccctcaccaa gtcgctgctc
     5761 ggctacgaca ccgtcaatcc gcccgggcgc gaagccgatt gtgcgcgcca cgccgcacgg
     5821 ctgctggcgg actggggcta cgacgtcaag acccacgatt tcgagccggg ccgcaccagc
     5881 gtggtcgcgc gcgcgggcgg caatggcggc agggcgccgc tgtgcctcac cgggcacctc
     5941 gacgtggtgc cgctcggcac gcgcgcctgg tcgcgcgatc cgttcaaggg cgaaaccgac
     6001 ggcgacaagc tctacgggcg cggcacctcc gacatgaagt cgggcgtggc ggcgatcatg
     6061 ctcgcggcga agcgcttcgc gaagaagctc cagggcacgc cgggcgtggt gctggtgctc
     6121 accgcctcgg aggaaaacgg ctgcatcggc tcgcgccacc tcgccgcgct gcccaagctg
     6181 atgggcaagg cgggcgccct ggtggtcggc gagcccacgt cgaaccatcc gtacgtcggt
     6241 cacaagggct cgctcaagtt ccatgccgat ttccgcggcg tctcggcgca cggctcgatg
     6301 c
//
LOCUS       DBNHKE010000164        60690 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1351251, whole genome shotgun sequence.
ACCESSION   DBNHKE010000164 DBNHKE010000000
VERSION     DBNHKE010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 60690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 60690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60690
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1351251"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..900)
                     /gene="rlmB"
                     /locus_tag="AC16O9_11055"
     CDS             complement(142..900)
                     /gene="rlmB"
                     /locus_tag="AC16O9_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR6517194.1"
                     /translation="MSRQIGREPRVVFGFHAVLARLRADPSSVLELYVDETRHDARIR
                     DVATVAERAGVKVLKVPTKRLDGFYGGGRHQGVVARVEVKSLSHSLDEILDAVQGPPL
                     LLLLDGVTDPHNLGACLRVANAAGVHAVVAPKDRAAGISAAVSKVASGAAEATPYLMV
                     TNLARTIDELKEREIWVVGTDERAEQSLYQANLPEAIAWVLGAEGEGMRRLTREKCDL
                     LVRVPMAGTVGSLNVSVAAGVCLFESVRRRLPKP"
     gene            complement(897..1295)
                     /locus_tag="AC16O9_11060"
     CDS             complement(897..1295)
                     /locus_tag="AC16O9_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR6517195.1"
                     /translation="MDTNVLVSAFATRGLSAELLQAVALEHDLLTGREVLREFARTLA
                     EKFKAPAERCAEAIETIVRNASLVVEESAPADCDADRDDRRILGEALASGAQIFVTGD
                     KQLLDLEAVGSMRILTPRQLWEQLRKGSGA"
     gene            complement(1304..1519)
                     /locus_tag="AC16O9_11065"
     CDS             complement(1304..1519)
                     /locus_tag="AC16O9_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR6517196.1"
                     /translation="MSTLTIRLDPKLDKELGRMARRTGRTKSELARDALRRQLAIVRF
                     REVRAKILPYAEAQGFLTDEDVFREIS"
     gene            complement(1553..3490)
                     /gene="rnr"
                     /locus_tag="AC16O9_11070"
     CDS             complement(1553..3490)
                     /gene="rnr"
                     /locus_tag="AC16O9_11070"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR6517197.1"
                     /translation="MSKKRRAEERTHRTERARAAPLALGKFSGRVEGHADGHGFLAPD
                     GGEPRIFLSAAEMRQVMHGDRAVVRVAGADHRDRPSGEIIEVLERVNRRVVGRLHSER
                     GVTFLVPEDRRIAQDILVPPADAGRAKAGQVVTVELVTQPSKTAQPIGRVAEVLGNYA
                     DPGMEIEIALRKFDLPHEFSKRAEAQARALPEAVREEDARGRRDLRRLGFVTIDGETA
                     RDFDDAVYAEKEGKGYRLWVAIADVSHYVRDGEALDQDARARGTSVYFPRRVLPMLPE
                     KLSNGLCSLNPEVDRLAMVCEMAITGAGKVARYEFYPALFRSHARLTYTAVWARLSGG
                     KAPQNLAVLYELFKVLHAARVQRGAIDFETLETKMEFDAHGKIARIVAEPRNDAHRLI
                     EECMLAANVCAGEFLAARGQPALYRVHEVPAEEKVRGLREFLAELGLQLPGGDKPRPK
                     DYAQLLERIKARPDFALLQTILLRSLKQAVYTPENVGHFGLAFDAYVHFTSPIRRYPD
                     LLTHRAIKAALANRRYEGVDWDELGRHCSETERRADDASRDVESWLKCHYMQDHVGGV
                     FEGAITGVTPFGLFVTLDEYFVDGLVHISELGRDYFEFDAARHMLLGSRTKKRYRLAD
                     RMKVKLVRVDLDSRKIDFVPA"
     gene            3524..3610
                     /locus_tag="AC16O9_11075"
     tRNA            3524..3610
                     /locus_tag="AC16O9_11075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3558..3560,aa:Leu,seq:cag)
     gene            complement(3614..4918)
                     /locus_tag="AC16O9_11080"
     CDS             complement(3614..4918)
                     /locus_tag="AC16O9_11080"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR6517198.1"
                     /translation="MSKAARNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAG
                     HTLVIGTKKTVLHLIPSGILRQNVDCFIGNGVVVSPQALIQEMDELEAAGVRGVVARL
                     CISEACPLIMPYHAALDAAREAARGAQKIGTTGRGIGPAYEDKVARRAIRLQDLFKPV
                     RFAEKLQGLLEYHNFVLTQYHGQPAVEFQKTLDEALALAPRLAPLVADVPRALYHANR
                     AGKNLLFEGAQGSLLDIDHGTYPYVTSSNCVSGAAAAGAGIGPHHLHYVLGITKAYTT
                     RVGSGPFPTELSDDVGEQLRRRGQEFGATTGRPRRTGWFDAAALKRSIQLNGVSGLCI
                     TKLDVLDGVEAVKICVGYRIEGELSEILPVGAEELERCEPVYEELPGWKESTVGIKKY
                     DALPQAARDYLKRIEGLAGVPIDLISTGPERDETIVRRHPFA"
     gene            complement(4948..5886)
                     /locus_tag="AC16O9_11085"
                     /pseudo
     CDS             complement(4948..5886)
                     /locus_tag="AC16O9_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
     gene            complement(5883..6068)
                     /locus_tag="AC16O9_11090"
     CDS             complement(5883..6068)
                     /locus_tag="AC16O9_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HIR6517199.1"
                     /translation="MATTFLLAFALMLIIEGVLPFLAPSLWRDTFRRVTQLSDGQIRF
                     FGLTSMLIGLLLLALAT"
     gene            complement(6075..6959)
                     /gene="hflC"
                     /locus_tag="AC16O9_11095"
     CDS             complement(6075..6959)
                     /gene="hflC"
                     /locus_tag="AC16O9_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR6517200.1"
                     /translation="MRAFGPVIAVLVALLLVASSVMFTVDQRQNAIVFQFEEVKDVIT
                     KPGLHFKWPLIQKVRLFDMRILAYDDAEPLRFLTQGNRPVLVDSFIRWRIVDVKQYFV
                     SVRGDELEATRRLRQTISGALRDEFGARTVHDVVSGERDAVMGRVREKADQDLRRIGV
                     EIVDVRLKRVDLPQEVSESVYRRMEAERKRIANELRSTGAAESERIRADADRQREVIL
                     AEAYRDAQRVKGDGDARAAATYAQAFSRDAEFYSFYRSMEAYRSTFRGRADLLLLEPS
                     SDFFRYLKDPGGRAGGKK"
     gene            complement(6961..8154)
                     /gene="hflK"
                     /locus_tag="AC16O9_11100"
     CDS             complement(6961..8154)
                     /gene="hflK"
                     /locus_tag="AC16O9_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR6517201.1"
                     /translation="MSLNDPNWGRGSRGPGGNQGPPDLDELWRNFNRKLNDLFGGRRG
                     GGGDIPPMPPSGRMLGGGAGVLVALVVVIWLASGFYVVDAASRGVVLTFGKFSEITDP
                     GLRWRMPFPIQSHEVVNLSQVRTLEVGYRNSVKTKVLKESLMLTDDENIVDLQFAVQY
                     VVTDAQAWLFNNRRPEEVAMLTAEAAMREIIGKSKMDAILYEGQDNIANQAQVLMQQI
                     LDNYKTGISLSRVTIQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDVIPRARG
                     AAARLTQEAEGYKARVVANAEGEASRFRQVLTEYSKAPAITRERIYIETLQSVLSSTS
                     KILVDTRASGNLLYLPLDKLMQQSGVSGLTESQASQRSSDAALPMDPGPRSRDSLRNR
                     ERGER"
     gene            complement(8154..9287)
                     /gene="hflX"
                     /locus_tag="AC16O9_11105"
     CDS             complement(8154..9287)
                     /gene="hflX"
                     /locus_tag="AC16O9_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR6517202.1"
                     /translation="MISAAAPDNQAAVLVSLDLGGAAPEDAAEELLRLAHSAGIRRHA
                     EVRGRRQRPDPKFFVGSGKVQEIARAAQGLNAATVIFNHALSPAQQRNLEKALEREVL
                     DRTELILTIFAQRAKTHEGKLQVELARLQHLSTRLVRGWTHLERQRGGLGKTGGPGEK
                     QIELDRRMIADRVRMLKGKLEQFRDRRVVQRRARARRDVLSVSLVGYTNAGKSTLFNA
                     LVHARALEADQLFATLDTTTRRIWLPEAGTVVVSDTVGFIRDLPHGLVAAFRATLEET
                     VHADLLLHVVDSASPVREQQALEVDKVLEEIDAGGIPQILVWNKIDLSGLQPGVERDE
                     YGRIGRVFVSARSGAGLADLRDAIAEAARARQPRTPSPEPISL"
     gene            complement(9284..9523)
                     /gene="hfq"
                     /locus_tag="AC16O9_11110"
     CDS             complement(9284..9523)
                     /gene="hfq"
                     /locus_tag="AC16O9_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR6517203.1"
                     /translation="MSSKGQLLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNSVTQMVYKHAISTVVPSRAISLGADSEKDA"
     gene            complement(9582..10667)
                     /gene="aroC"
                     /locus_tag="AC16O9_11115"
     CDS             complement(9582..10667)
                     /gene="aroC"
                     /locus_tag="AC16O9_11115"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR6517204.1"
                     /translation="MSGNSFGTLYCVTSFGESHGPAYGGVVDGCPPGLELSAADLQQE
                     LDRRKPGSSRHVTQRRETDTVEILSGLYEGRTTGTPIGFLVRNEDARSKDYAAIAEKF
                     RPGHADYTYWQKYGVRDPRGGGRASARETVVRVAAGAIARKWLGVRYGVQVRGYLAQL
                     GPHRIAFRDWDAVSANPFFAPDAGAVAELEKFMDQLRESGDSCGARVNVVAHKVPVGW
                     GEPVYGRLDADIAAAMMSINAVKGVEIGAGFAAVAQKGSEHGDEMTPQGFLTNNNGGV
                     LGGISTGQDVLVSIALKPTSSIRLPRRTIDVHGAPATVSTSGRHDPCVGIRATPIAEA
                     MLACVLMDHALRHRAQCGDVTPPTRTV"
     gene            complement(10678..11772)
                     /locus_tag="AC16O9_11120"
     CDS             complement(10678..11772)
                     /locus_tag="AC16O9_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6517205.1"
                     /translation="MRAAWGGIDAAQVWDAHAHLVGTGDAGSGVVVNPRMESLLNPGE
                     YARRLFFLNAGCVHDAPGSVDRAYVDRMLNLMAGMRAGTKLLLFAFDRAHDERGRVQW
                     ERTGFYVPDAYARDTARAHPQVFEWAASIHPYRADCVEALERAKADGARAVKWLPAAM
                     GMDPASALCERFYAAAARLELPLISHAGRELAVVGSDAQDHGNPLKLRRALDAGVRVV
                     VAHCASLGEDRDLDRGPGGPWVDSFALFGRLMDETRFAGRVYGDISAITQVNRAGGTL
                     VRLLERTDWHARLLNGSDYPLPGIMPIFSVDYLVALGLLAASAAPVLTEIRAHNPMLF
                     DFVLKRSLRRNGSAFAARAFETRRFFEGAV"
     gene            complement(11906..12340)
                     /locus_tag="AC16O9_11125"
     CDS             complement(11906..12340)
                     /locus_tag="AC16O9_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6517206.1"
                     /translation="MQVKEILRVKGNRLLTIEPSGRATDAVKTMAEQNLGSLIVLEQG
                     RMVGVLTFREILQALAQRSGTLAELRIAEVMVRDPVTATPDMEVNDLRRTMLESGARY
                     LPVVQDGKLLGVISFRDVAKAVLEEQDFENKMLKGYIKNWPA"
     gene            complement(12432..13658)
                     /locus_tag="AC16O9_11130"
     CDS             complement(12432..13658)
                     /locus_tag="AC16O9_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6517207.1"
                     /translation="MESIDRPTIQRSAIDLAEDVKLRAPQGAAEKLAHYGGAEVAAVL
                     LKLSPGFTQDVLTYLPADTRERALAAAPVEIARQWQRNALYAEGTIGRMMEPLYAAFP
                     PQATVGEAIEALHELVKHAFITYIYIVDEDERLLGIVTMRDLLFNDRARALRDVMLTD
                     VFALHAPTVLDEAMKQVLDRHYPVYPVVDAENRIRGLVRGQSMFEARAFEIAAQPGSM
                     VGVEKEERMGTPWQRSLMMRHPWLQLNLLTAFLAAAVVGIFQDTIDRLVILALFLPVL
                     AGQSGNTGCQALAVTLRGMTIGELKPGSERALITKEAGLGLLNGAGVGLVAAIGMYIT
                     ASSQNSAHPVLLGFVVFMAMVGSCVASGLSGAMVPLTLKRLGFDPATASSIFLTTATD
                     VVSMGMLLGLAALLVS"
     gene            complement(13685..15064)
                     /locus_tag="AC16O9_11135"
     CDS             complement(13685..15064)
                     /locus_tag="AC16O9_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6517208.1"
                     /translation="MKPNLIVILIDDLRYDEFGAGGHPYMKTPHIDRLAAEGVLFERA
                     FHTTPICSPNRASIVTGQYASRHGIIDNVARDAMSHRLPNYHLALQKLGYDTAHVGKW
                     HMGNDGMPRPGYAHWVSFDGHGSLVDPRLNVNGRYEQRPGYMTDLLNQEALAFVVRKR
                     AKPFALFFAHKAVHPDAHQAADGTLDMSRFGGYIPAERHRALYADGVFPKTPNMRPTH
                     ELVKQAPAWTEAFELKGSERSRALLEAIQAGSQDEIRQRARMMASVDEGVGQLLDALA
                     RQKKLDDTAIVFLGDNGYFFGEHGLGPERRFAYEEGIRSPFVVRYPRKARAGTRNRDL
                     VICQDIAPTFVELAGGRPGAHVQGRSLLPLLAGRRKGWRKSFLVEYWAEQAMPWLVGM
                     TYKAVRTDRYKLIRWVNRGAAGELDELYDLARDPWELKNLARGARHGALKARLRRELR
                     KLVADAAGL"
     gene            15177..15737
                     /locus_tag="AC16O9_11140"
     CDS             15177..15737
                     /locus_tag="AC16O9_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517209.1"
                     /translation="MAMTQPQVSVPQWLGRQLLGPLDDAAGLVKRYRELDAFRDYLAA
                     RVRLVAPALALVVVAALACGLAPIVVFLSTNAAAALVGLLLAPVMLIGSLFVLSLVLF
                     AWLEERSLARALGHRTGPAPGRLARWVKRKLGADIGSALRVPWLLGALFVVAPLALLA
                     TRAPGTVALLVALLALCPILFARLDR"
     gene            complement(15859..16152)
                     /locus_tag="AC16O9_11145"
                     /pseudo
     CDS             complement(15859..16152)
                     /locus_tag="AC16O9_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            16213..18777
                     /locus_tag="AC16O9_11150"
     CDS             16213..18777
                     /locus_tag="AC16O9_11150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6517210.1"
                     /translation="MRAGAALLALLAAHVPAASAQSSPRLEPGVSLELARWRAQHYRD
                     PAYALELKIDARRRRASGTLELQVTLARRVDLVLDWRGAPVRGLRVNGKPVRARQERQ
                     HLVVPRTALVRGRNRVELAFRAPIAVSGRALTLYRDREDGASYVYSLFVPADASSVFP
                     CFDQPDLKARFRLALELPRGWRAISNAPLLEEAPGRVRFAETEPISTYLFAFAAGPFE
                     AVTQVGEPVRLFVRRSQLERAREHAGEVLRLNRLALAFFEAEFARRFPFAKYDLVLVP
                     ELAYGGMEHAGATFLNEQSILFPDPPSGPDLLRRAQLIFHEASHQWFGNLVTMRWFDD
                     LWLKEGFANFMAAKAAAAIVPELEPWSAFHALKTSAYRTDATAGTTPLRQPLANLSSA
                     KSAYGSIVYSKGPAVLRQTEFYLGEHVFRRAMRDFLQRHAYGAADWSDLVRAFERVER
                     RDLQAWAAAWVQRRGLPTVTLRETPGGVLLAQEDTLGEDGVWPQKLVVAAATAEGSVV
                     TSEVRLEQASATVGGPAGARVVRWRYPNAGDFGYGRFLLDAASRDALLAEPRALPDGL
                     LRAQLIEALWESVRDAELDPTAFIDFALVLLPEEGDDVILAGLLARVEGAFRRYLSEA
                     QRGRLAPRLERALLGAALGEGPESRRRLMLRAFVALAWSGPALADLKRLHEGSLSAPG
                     LELGERDRFRILARLLVRADADGPRLLGEAAAAERGDNARRYAFAASAALADAEAKRA
                     MYRRFTRDAELPERWIEEALASFNAPEHAALTAPLLSAALAQLPELKRRRKIFFVEHW
                     LDAFLAGQTEPRALAAVRRFLRRDLDPDLRRKVLEAADGLERTVRIRERFAASAER"
     gene            18810..19313
                     /locus_tag="AC16O9_11155"
     CDS             18810..19313
                     /locus_tag="AC16O9_11155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6517211.1"
                     /translation="MTAHAISVRLAGQRDAQAIALMSRDLVEAGLGWKYDAARVLKAI
                     RDPETLTPVACDRGRIAGFAIMEFGDERAHLVLLAVRPTQRRMGIGRRLVDWLVESAR
                     TAGIASVHLELRAGNEAARGFYRAMGFDETIVVPGYYRGREAALRMIRVLRSTSPVPY
                     TWRPSTT"
     gene            complement(19324..20145)
                     /locus_tag="AC16O9_11160"
     CDS             complement(19324..20145)
                     /locus_tag="AC16O9_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014324.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - glycerophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HIR6517212.1"
                     /translation="MQRWPLLLCALAALPAAAQDQPRPDVMETFIVNQEREGHEPPLT
                     ENEPVYFVMGTRGGTNARFQLSFKYRMFDRGLGWGGEQPWLSGFYLGYTQTSLWNLSG
                     DSKPFRDTSYWPSLFWDWKRAGDKTWIDGLRAGFEHESNGKDGAQSRSIDMLFVHPEW
                     VWLLDNGKRLELAPKLYGYLDKEENPDIQRYRGYVDWRLRYGDDVRSWRAMARLGTAG
                     KGSLTLDWFERTRVLGFGPVSGYFHVQFFAGYGEDILDYDRRFKSQLRLGFAIVP"
     gene            20168..20992
                     /locus_tag="AC16O9_11165"
     CDS             20168..20992
                     /locus_tag="AC16O9_11165"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016701 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR6517213.1"
                     /translation="MTESSAPLPAVFISHGSPMHALEADASATGRAWRALGARLPRPR
                     AILIASAHWETEQPALTGTARPETIHDFRGFPPPLYEIRYPAPGAPDVAGRAQQLLGA
                     AGLAASIDPRRGLDHGAWTPLLYVYPAADVPVAQVSVQTALGPRHHLALGAALAPLAR
                     EGVLIIGSGHLTHNLRELRSVRETGVGRAEPYVAEFQEWVRECIETHDYGRLADYRQL
                     SPAGVRAHPSEEHFLPLFVALGAAGGEARAERIVGHVEGGVLAMDAYLLSPGPEKS"
     gene            21094..22392
                     /locus_tag="AC16O9_11170"
     CDS             21094..22392
                     /locus_tag="AC16O9_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6517214.1"
                     /translation="MLGLARFEGDMEVRFRAQYAERSIPFVRVSLPLAATLYLLFLAW
                     DYSIAPEALLYTLSVRLPFSFFAVAVFGFTFLHTFERWSQSVLCATVILGAAGIALVL
                     AILPQGFTYGVPGLLLVIMYACGAIRLLFTSAFIACCTIVALTNVLLLTTGASTFQVL
                     NTNVFLIAASAIGLIYTALLEWTERHAFALEEDLRKDKQASETLLRNFLPDRIMSRLR
                     DGERTIAEAVGEATVLFADIVGFTSLTHRMAPGHVVEVLSVIFNRFDEISDQRGVEKV
                     KTIGDSYMAVAGVRSPSPRNAEAMAEFAIEALAFVRSYAMENDLPLQVRIGMSTGSLV
                     SGVIGTHVPIFDLWGAPVNDASRLQQEGIPGAIQVSETTYWRLRNKYEFQARGTLTLK
                     HGEQINAYLLTGRKVAAGAGLHVISDASRATADTLPAKTG"
     gene            22394..23479
                     /locus_tag="AC16O9_11175"
     CDS             22394..23479
                     /locus_tag="AC16O9_11175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6517215.1"
                     /translation="MDATLGQQQVARADATARAAGWLCPEYREVVDRTVELLLNTKAR
                     LRAAVPAGADGGRAIETITNDLWAEWRSAWVDAAASLAEIAATPEHLRLAKDYTESHL
                     TPELMGAPLWRQAYTKPLGYPGDYIAMNYMYDGTPEGDTAFASVAHELAVHIGQFVVK
                     RKDLVRHAIAEAVARHSGAEPCVIASLGCGPSREVTEFLAHDPLASRPTRFFLVDQDS
                     DALSFAGRQLAAVVRGGAGQGAARIEMRHLSVLRLLREIEPGELLTPADLIYSAGLLD
                     YFSDRTCRILTQRLYQALRPGGHLLLGNMKAGTDMLWPLEMIADWTLRYRNAESILAW
                     TEGLEGAEISLRTEATGYDYLLSVRKA"
     gene            complement(23570..23941)
                     /locus_tag="AC16O9_11180"
     CDS             complement(23570..23941)
                     /locus_tag="AC16O9_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR6517216.1"
                     /translation="MGNPAPGFKNAQRNHIVTRPARKRVRVTLGAEVLADSREALEMR
                     EGSYPPVYYVPRKDVKLERLMRTSLTTHCPYKGDASYYCIVNGPDNAVWSYEQPYDEM
                     LDIRDLLAFYPNKVAIALGDD"
     gene            24018..24692
                     /locus_tag="AC16O9_11185"
     CDS             24018..24692
                     /locus_tag="AC16O9_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517217.1"
                     /translation="MPARRRFARREDLGLTKAEFAVLRRLNTPRKAQAFLYGLPQNFE
                     LRGDTCNSVRDVLRTRRAHCIEGAMLAAAALWVHGEPPLLLDMRAVRDFDHVVALFRR
                     RGRWGAISKTNGATLRWRDPVYRSLRELAMSYLHEYCNRRDHKTLREYSRPFDLRVLR
                     TADWVTAPKGAWAVAERLDALHHYRLLSPAEERALVRRDPFERRIGALLQYRRPKRLR
                     RGSGRY"
     gene            24816..25136
                     /locus_tag="AC16O9_11190"
     CDS             24816..25136
                     /locus_tag="AC16O9_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517218.1"
                     /translation="MLKKTAAIAALAGATLLSTTPALADGYRHGYGHGPKRVVVVHKQ
                     VPYYGYRQGYRQAVAYRPVYAHRPVVVHRPVVVQHRYRGHDVLGGLIVGAVLGAVIAS
                     HGGY"
     gene            25324..25797
                     /gene="msrB"
                     /locus_tag="AC16O9_11195"
     CDS             25324..25797
                     /gene="msrB"
                     /locus_tag="AC16O9_11195"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR6517219.1"
                     /translation="MAMVEELQRNWKMFLAAGVATPSPEEPLTLSKDEWRKRLPPAAF
                     HVLREEGTERAGTSPLDAEKREGVFACRGCDLPLFTSAMKFDSGTGWPSFFTTIPGAF
                     ETKTDYFLIYPRTEYHCKRCGGHHGHVFKDGPPPTGLRYCNNGVALKFIPKEGKA"
     gene            complement(25794..26597)
                     /locus_tag="AC16O9_11200"
     CDS             complement(25794..26597)
                     /locus_tag="AC16O9_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517220.1"
                     /translation="MRAAADNIVEIRDLHFAYGENQVLRGLSLSVPRGKVVGILGTSG
                     CGKSTLLKLIGGQLRPLRGTVHVDAQPVHELDGAPLYDLRRKMGMMFQTSGLFTDLSV
                     FENIAFPIREHTRLPDALIRSLVLMKLHAVGLRGAHALRVGDLSGGMTRRVALARAIA
                     MDPMLTMYDEPFAGLDPISLNVIADLIRTLNDALGATSIIVTYDVHESLKVADYLYVI
                     SEGVVIGAGTPAELRDSKDARIYQFLHMEPDGPVKFHYPAAPLAGALGL"
     gene            26624..27607
                     /locus_tag="AC16O9_11205"
     CDS             26624..27607
                     /locus_tag="AC16O9_11205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6517221.1"
                     /translation="MTAPAPYAGLTPETVLDALQVAGLRGDGRLLALNSYENRVYQAW
                     LEDGGSVVAKFYRPERWSDAQILEEHAFVQELAAREIPAVAPLALEGRTLNAHAGFRF
                     AVFARRGGRTPELEDPETLRWLGRYIGRIHAVGSGRGFDARPALNVETFGREPRDWLL
                     AHGFLPPDLREAWQSAAALALQGAARCFERAGSVRILRLHGDCHAGNVLWTDAGPHFV
                     DFDDARSGPAMQDLWMLLSGDRASMARQLGLVLEGYRPFFEFERRELHLLEALRTLRL
                     LHYSAWIARRWDDPAFPAAFPWFNTQRYWQDRILELREQIALLDEPPLELA"
     gene            complement(27604..28059)
                     /locus_tag="AC16O9_11210"
     CDS             complement(27604..28059)
                     /locus_tag="AC16O9_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HIR6517222.1"
                     /translation="MAPRLEFLMKIAADVGELQTLGGGPLGERRVVAITGGTFEGPEL
                     KGKIVPGGADWQIVRADGVLDIDARYAIRTDAGALIRVVSQGYRHGPPEVLAALGRGE
                     DVPAEQYFFRTVMRFETGDAGALWLNRTIAIATAQRRARQVLLEAWRLL"
     gene            complement(28062..28697)
                     /locus_tag="AC16O9_11215"
     CDS             complement(28062..28697)
                     /locus_tag="AC16O9_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6517223.1"
                     /translation="MGTSRICIIRHGETAWNAEGRVQGQTDVPLSAVGEAQARAVCEA
                     LAGERFVALYSSDLARVRQTAAPAAARLGLPVRLEPALRERHYGKFETLTYAEARERL
                     PEDYARFKAKDPDYDFGSGESLRGFYERAVACVASIAARHAGETALVFTHGGVLEMVH
                     RHAHGTGLSAPRDFAIPNAALNWIEIGQQAWHVRSWAQVAHLERALDDLPD"
     gene            28735..29856
                     /locus_tag="AC16O9_11220"
     CDS             28735..29856
                     /locus_tag="AC16O9_11220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6517224.1"
                     /translation="MTDSPPAHHSALPSIEQEELRGISRTVAEIEWLLLVLVLVYVVF
                     GRPPVEVETALSMALFFFAAFVMSFRYTNFYKRETRWKIAVETLAMIVFITWALWFTG
                     GPASPLLNAYLLVVITAALTLGKTVTLIEVALIGACIVLLDLQVGGKDMMQLAYLGNF
                     VAQLAPVILVAYITTMFSADIRYGLNRAKLLSETDELTGLYNMRGFIVTANRLFAQAQ
                     RYARSASFLMVDSDSLKQVNDSYGHEAGNRLLQHIVNSMQGHLRATDVPARYGGDEFV
                     IMLPDTPARGALEVAERIRNAVEATPFATEAGRVPCTVSIGVASYPQDGRSIEALLAR
                     ADRALYEAKAAGRNRVAQHDVAMQCGAQPAGGFQIPNST"
     gene            complement(29835..31190)
                     /locus_tag="AC16O9_11225"
     CDS             complement(29835..31190)
                     /locus_tag="AC16O9_11225"
                     /inference="COORDINATES: protein motif:HMM:NF018352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HIR6517225.1"
                     /translation="MRAAGFILLTMWLGVASAYGDRFDAALDVGGAGSGSAVRAERSP
                     YLGDGVRYDYLPLNVYAGEHAYLHSDRVGLKLDLDADQRVDVFLAHRYEGTPLVRAPA
                     SLAGMALREQGTDLGLGYRRRMAWGALGVELLHNVDDASRGTEVRLRLAEEIRRGELR
                     LWPQAMLAWRNTKLNDYYYGVRASEASAARPAYVADSGLNLELALYASYPLSRNWQLL
                     GGISATQWSSSVRASPIVENRVQAAATVGLLYEHSPEPAAWSASRLLWVKVYHGKASE
                     CDVLQIVKLSCTSTRTPEETRVTSVELGRTLIERLYGWNMDIAGYIGVLHHDENGFQP
                     DHWQLNAYFKSYWYGFPWRERVKTRIGIGSGLSYAERVATIEARDQARRGENTHKLLT
                     YLDPTIDFSVGDLVGSRALKNTYVGLGVSHRSGVFAMSRLLGSVDGGSNFIYTYVEFG
                     I"
     gene            31283..32377
                     /locus_tag="AC16O9_11230"
     CDS             31283..32377
                     /locus_tag="AC16O9_11230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6517226.1"
                     /translation="MPLRHLMPGTLVVCACLAAAPATAMEPWNLLAQSRKQTPQPPWS
                     TGDERGMANQIGPATWARCAWHLGQRSAKAYELSHERSSTMPLSPFTGPYVQTPKLTA
                     GLPGTGHGFNGESLNEGAEPAAQGTQMDALGHFAFMTQPWDGKPPFPADTLRYYGGFT
                     QADVKPTPQSPLARLGMEKAPPIVTSAVLLDAKTHVGGGAAMKAGEVVTRKDIEAMLR
                     AQGLGKRGIQYGDVVYVRTGWGERWRDPDTEKSYYAKAPGLSYDAAQYLGERRVVAVG
                     LDTPFIDPVPEGMLTGKASPAPGTPEGLPFAVHHHLLTQMGIHHIENAKLGELANDKV
                     WTSCTMILPLRERGAAGSPVRPVAIGVPGR"
     gene            complement(32385..33869)
                     /locus_tag="AC16O9_11235"
     CDS             complement(32385..33869)
                     /locus_tag="AC16O9_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HIR6517227.1"
                     /translation="MRLVIAQMKHETNTFSPVATDLARFAGGRAAPLEGEAIRAAFKG
                     TGTALGAFLDLAEKADAEAVLPVAGNAAPSGPVQRAAYEHMAGRIVDAVAKGCDAVLL
                     DLHGAMVTERHDDGEGELLARIRRVAPQVPIGVALDMHTNLFPAMGEHATVIAGYQTY
                     PHIDMYETGLRAARPVLGMLAGRPRPAMAWGWRPMLPHVMRQSSLDEPNRSIQARARQ
                     MEAKGEALSATFFVGFPHADIPYAGVSAVVVTEGDRARAQRLADELLDMAWHSREQFV
                     YRVEPLAASLERARSLQGGPIVLLDHYDNCASGGTMDTMTVLGGILEARLADVCAFAV
                     FDPDAVQKMKAAGKGARVTLPLGGKLDMPALGLKGRPLEVSGTVTRLIDGTYRNEGPM
                     AKGERMDMGPSAVLDTGRVQVAVISRHTEPYDLGAFRALGLDPAKKRYVMLKSRVHWR
                     AGLGPLAHAVVECAGEGVCTSDYAQLRFKRVRRPAYPLDPDARA"
     gene            33903..34862
                     /locus_tag="AC16O9_11240"
     CDS             33903..34862
                     /locus_tag="AC16O9_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6517228.1"
                     /translation="MRAYFIRAGNGQARVESKDAALPEPGPQQMRVRVRAAGLNRGEF
                     MGAGATAKPAGTDAAGEVEKLGPGVTRFKAADRVMGRCPGAFAEYALMDERETMAVPE
                     KLSWEDAAAVPIVFLVVYDMLVAQGRLKADEWLLVTGVTSGVGVAALQTAKALGAKVI
                     GTSGSKDKLAKLAPLGLDLALATRKPDFADAVLDATGGKGANLCVNNVGGTVFAECVR
                     ALAFEGRLAVVGYMDGVLKAEIDLDALHAKRLTLFGVSNKLRNAEQRATTVRGFSDKV
                     LPLIAEGRIRPLIDRVYPFDELPAAAERLQANAHVGKIVVILK"
     gene            complement(34912..36105)
                     /locus_tag="AC16O9_11245"
     CDS             complement(34912..36105)
                     /locus_tag="AC16O9_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6517229.1"
                     /translation="MTKLGGSGALSQLRLLDLSRVRAGPTCVRQFADFGADVIRIESP
                     PGLDPNEGMGGARTGSDFQNLQRNKRSLTLNLKQPQAKEVFGRLVKTADVVVENYRPD
                     VKFRLGIDYESLAAINPRIILASISGFGQDGPYRDRPGFDQIAQGMSGLMSITGLPGQ
                     GPVRVGAAVADVSAGLFAALGIMTALLERERSGKGQWVQSSLLQAGITLTDFQAARYL
                     KEGEVAPQVGNDHPTSMPTSAYKTADGHINVAASGEGMWTRLCATLGREDLLARPEFK
                     GQPARAKNRAALNAEINAALGKKKSAEWIEALNAAGVPCGPIYTMDQVFADPQVNHLG
                     VPVEVESRALGKFRVVNQAVKLSRTPARMLRAAPEAGEHTDEILAELGYGADEIASLR
                     AAKAI"
     gene            complement(36131..36502)
                     /locus_tag="AC16O9_11250"
     CDS             complement(36131..36502)
                     /locus_tag="AC16O9_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517230.1"
                     /translation="MREILGLGDEAPLRRRWFHDEYFDLFVWQDALGALARFELCYGA
                     GASERALVWLGGERLFHDGAPGAAPDLQVLLQRFERAGGGLPAEVRREVLARIREYAA
                     RPVARRSGYRRAAWQQRDAQA"
     gene            complement(36544..37839)
                     /locus_tag="AC16O9_11255"
     CDS             complement(36544..37839)
                     /locus_tag="AC16O9_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6517231.1"
                     /translation="MPDTIDLGLSLQVGKYDVHKLLGKGATGTVYLARDTFSGGDVAL
                     KTIEPEVFRDPEFGTVYRSQFQNEASLAGKLKHPHIVSILDAVVGEDSGYIAMELVTG
                     GDLAAHTQPGKLLPVADVLQIGFKCCGALDYAFREGIVHRDIKPANLMLAGGTNVKIA
                     DFGAAFLRKTQAVQSAEMGSPYYLSPEQIAGHELTHHSDMYALGVVLYEMLTGQRPLR
                     AENLDALLKKILELEPLPPSEVRRDLPRELDAVILRALRKKPEARYPTWAEFAAELAK
                     VVRQSVPPSAIPDTEKYVALKRVEMLSRLDDADLWELASAGKWTRVEKGRTIVRENDK
                     GKSFFFLAEGEVKVTKAGRLLNVVSHSECFGEMAYIWGGELPRHATVESMTKLLLAEF
                     DHAALARMSGGAQLQLTRALVRNLVDRLVLANTRVTAVR"
     gene            37955..39190
                     /locus_tag="AC16O9_11260"
     CDS             37955..39190
                     /locus_tag="AC16O9_11260"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HIR6517232.1"
                     /translation="MEHKEVPVEYLEFGMFVAKLDRPWTETPFVYQGFVLKTEQQLEA
                     LKKYCKVVYVDPERRQEVVAARLLSLPPGSTRQVIAEQSKEKVRGTVIYKELATVEDE
                     LPRARSVFTQTNAIVHTMSRAVVTGNALDGNRARQAVTEITDSVVRNPDAMMLLVTMR
                     DKAEETLNRSTQVSVYMTVFGRFLQLERERLEILGLIGLLQDVGMLKLPAGIPGRRSD
                     LAQEEDAVFKSHVTHSVEILSQTAGLPPELPSLASLHHERYDGSGYPRGLKGGAISQT
                     GAIAGIVDTFDMLTAPAPYGMQMPPSNALNVLFQNRGTQFHAALVEQFIQCVGAFPVG
                     SVVELNSGEVGVVITQNLVKRLQPRVMVVQDANGNPIHPHKVLDLVNEPMATADEPYR
                     IRRTLDQSTVKIDSRELFL"
     gene            39187..40941
                     /locus_tag="AC16O9_11265"
     CDS             39187..40941
                     /locus_tag="AC16O9_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6517233.1"
                     /translation="MNSALDRAAILRLLEQFGLGDDALAALEALGAALSRQSPEALQA
                     ARGESALAGATSPFFLPLLPAAMTESQRALVGAIARGALGGEALPQLLLDWAATLNPE
                     EPSERALRDCRPLQELLRSGALHVYAGDAASCAAALAAAERLGAVQLALIAETAARGA
                     PGGAPAAFGLSDFMAQVDACVAARIADGRQMALLFVDCGVIGRIDGVWGYVVGDGVRE
                     RLGARLRTEVLRTQDLLGHPGRDQFICVLADLHSAGVATLAAEKLLRALDQPLWVDVD
                     ELYPRPAVGIAVYPEHGTSGVELLQRARVSADAARERPERYALYSADQERSEIELLLY
                     ENRLRAAIAQDALHIVFQAQRDARTGLIVGAESQLRWRDAGLGAVPMEKAIAAAESAG
                     LVNEVTLWLVNGALRNCRQLRDTAGLDLRVGVTLSAKSLRQPDLPDFVLSALKTWNLR
                     PSRLSLEITDTSVLAASLESVETLQRLREAGVRLSLDDPNAGYASLAWLATLPFHELR
                     VDLANIGNIVDSQEHLALVRALVELAHRLKMDVLVAGVDDEASAARLETLGCDLMQGT
                     FISPPLETTAFVDRFRGD"
     gene            40956..41333
                     /locus_tag="AC16O9_11270"
     CDS             40956..41333
                     /locus_tag="AC16O9_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517234.1"
                     /translation="MGDFGIGQPVERFEDPRLLRGAGRFVHDLSLPGQACLVLVRSPH
                     AHARIVALDAAAARAAPGVLGVYSVEDLRADGTGTSAVALARRRAVRAARREPPGADG
                     AQPARGEGRRRSGHRRRAQGAQK"
     gene            41493..41876
                     /locus_tag="AC16O9_11275"
     CDS             41493..41876
                     /locus_tag="AC16O9_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR6517235.1"
                     /translation="MRNASLAVLLATALAACSEAEPPKSAAAPEPPKPVVLAPAPAPT
                     PSPAPATAPVVSPEEQLAERVKQALRDTRKVEGQGVGVRVAGGTVTLYGTAPSADERR
                     RIEQFVAGVEGVQAVVSKLVIVRGS"
     gene            complement(41845..42324)
                     /locus_tag="AC16O9_11280"
     CDS             complement(41845..42324)
                     /locus_tag="AC16O9_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6517236.1"
                     /translation="MPCPQLPGVPLHVVLRRSAADPAACLERLRAAAGCAVHAYVLMG
                     THAHLLLTPLGADGAMPLVHAMRALAADATPVYARRYVLACMRYIELNPVRAGLVRHP
                     AHYRWSSYRANALGEADALVTPHALYCALGRDGEERRAAYRGGFRAAATSRARSPAC"
     gene            complement(42371..42799)
                     /locus_tag="AC16O9_11285"
     CDS             complement(42371..42799)
                     /locus_tag="AC16O9_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517237.1"
                     /translation="MDLQAVLKKSGKGVEEIETRKHRLEQKLRTLLIVVNGKATGADL
                     VKQFEQLGDIRPMLERLLADGFVEAAAPAADFKEIRIQLAQALTEALGPEGDPIVMQL
                     DACKSPDEARAFVERHRPMLEKVVGPRGAAFLRKAAELLR"
     gene            42904..43185
                     /locus_tag="AC16O9_11290"
     CDS             42904..43185
                     /locus_tag="AC16O9_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517238.1"
                     /translation="MSTPEKAAAQAPHDPIQELAEKIYVQLCGRIYGQGGEKPQPKAV
                     AQLCFKLAETFIAANYEFNPTAIAAREAKNKASVKLDSVQIDFGAVDKG"
     gene            43339..43467
                     /locus_tag="AC16O9_11295"
     CDS             43339..43467
                     /locus_tag="AC16O9_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517239.1"
                     /translation="MFALPALANNEIDSAYTGAAQKSTPIAPVLANAELGTKGNQL"
     gene            43618..43980
                     /locus_tag="AC16O9_11300"
     CDS             43618..43980
                     /locus_tag="AC16O9_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR6517240.1"
                     /translation="MAKLARPLLETLDARERALRDKIAERREALEDASRPADPAGDEA
                     DKAFERTCAEVEHDLIEMSLRELTEIAALRERLANGTYGECLECGEPIPPARLEVNPT
                     ARRCADCQARHEKLSGAL"
     gene            complement(44075..44848)
                     /gene="ppgK"
                     /locus_tag="AC16O9_11305"
     CDS             complement(44075..44848)
                     /gene="ppgK"
                     /locus_tag="AC16O9_11305"
                     /EC_number="2.7.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF045942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0047330 - polyphosphate-glucose
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate--glucose phosphotransferase"
                     /protein_id="HIR6517241.1"
                     /translation="MNGRGTAIKVLGIDVGGSGIKGAPVDTRSGRLLARRRRIATPQP
                     ATPEAVARAVAQLAARFAWKGPLGCALPAVVKHGRVRTAANIARSWLGVDAHALLGAA
                     VGRAVGVINDADAAGLAEMAFGAGRGRGGLVLMVTLGTGIGTALFIDGRLVPNTELGH
                     LTLKGRDAETWASAAVREDESLSWKKWARRVDAYLHLLQRYFWPDLIIVGGGVSRRWR
                     KFLPRLTLPTPVVPAQLRNDAGIVGAALAAAQARRRTRR"
     gene            complement(44845..46482)
                     /locus_tag="AC16O9_11310"
     CDS             complement(44845..46482)
                     /locus_tag="AC16O9_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HIR6517242.1"
                     /translation="MAAERELSPRALLTGVLLGALLAPCNVYSGLKIGWSFNMSIAAL
                     LAGLAFWRAAERAARAPQWTMKESNISQTAASAAASIVSGGLVAPIPAYTLITGRGFD
                     PWPLVAWVFTVSFLGVWVAWALRPALIVQHPLRFPAGVATLETRRDVFAHGREATARI
                     SVLAGAALAAAAWKAFAPLAAWAPSAAAARLTIGLDPSPLLAGFGAIIGLRAGLSLAA
                     GALVAWGALAPWLLGTGRVAQGGFGPLVEWLLWPGVALMVTAAATRFALQVRGARLTI
                     GAHWTPVAVAGLALACALAVAMQVMLFDIGFVIALLAVPLALVLAAVAARVVGETGIP
                     PIGAIGKVSQLGIGLAAPGQAVTNLMAANVAGGAAGQCADLMNDYKVGHAIGASPVRQ
                     SVAQVCGVLAGALVGTVVYLALIPDPAGMLLTAEWPAPAVATWKAVAEAMSAGLTAVP
                     ASARIAMALGALAGVALGALEPRWRYAPSAVAFGLAFVIPASICFTMALGALAAWLLE
                     RRFASLAARFTIAAAAGLVAGESLVGVAASFLGIAAA"
     gene            complement(46495..47667)
                     /locus_tag="AC16O9_11315"
     CDS             complement(46495..47667)
                     /locus_tag="AC16O9_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HIR6517243.1"
                     /translation="MSRLNIDVTYLADVLKRLLETPSPSGYTDTIVRMTCDELGKLGV
                     NYELTRRGAIRARLPGAEQKGARAMIAHLDTLGAQVKNLKDNGRLEVVPIGHWSARFA
                     EGARCTLFTERGSYRGSILSLKASGHTFNEEVDTHPVGWPHVELRVDALARTRRDLER
                     LGIAIGDTIAIDTRAEFLANGFIVSRHLDDKAGCASMLAALKALQEDGGKLPVDMLFI
                     FSISEEVGVGASSVLTHDVASLVSIDNGTAAPGQASDEFGVTIGMADQTGPFDYHLTR
                     KLIKLCRENDIGYQQDVFRYYRSDSASAVEAGHDVRAALIAFGIDASHGHERIHMHAL
                     RSLAELVTAYATSPVEIPRDAEDISSLKGFTKQPMKEAKSTLLTPSGPAAAAEEKR"
     gene            complement(47679..49433)
                     /gene="ngg"
                     /locus_tag="AC16O9_11320"
     CDS             complement(47679..49433)
                     /gene="ngg"
                     /locus_tag="AC16O9_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HIR6517244.1"
                     /translation="MEAKVNRARPKRRPHAAYTHRVQRKYVAHVPDSEVALDCGWGRL
                     LFAQTYPDARSLAAALAQEGPERRDIASWVRDPHLVLAAAPQALFLDPSNTYRLDLST
                     YRGSRKRPRGFVVRWVTSQADADAVNRIYLAHRMVTVPAEFMFSHRDSRTIVYLVAED
                     EVTQQVIGTVTGVDHLRAFGDPERGASLWCLAVDPQAARPLVGETLVRRLAEYFQARG
                     VRHMDLSVMASNRQAIELYEKLGFTRLPVFTVKRKNAINERLFVGAPPEAQLNPYARI
                     IVDEARRRGIGVEVLDAEGGFFRLTYGGRSIACRESLSELTSAVAMSLCDDKAATRRA
                     VARAGVRVPDEIEFDTRESLAAFLAKHGSVVVKPARGEQGRGVAVGLETLEEAMAAVE
                     RAQAHSDRVLIEQCVTGEDLRLVVIGYKLVAAAIRRPARVIGDGASTIRELIERQSRR
                     RAAATGGESKIPLDAETERCVAKAGYTLDDVPPSGQEINVRKAANLHTGGTIHDVTAE
                     VHPSLIDAAIRAARALDVPVTGIDFMVRNPRQPDYWFIEANERPGLANHEPQPTAERF
                     VDLLFPHSMPTAVKRAAR"
     gene            complement(49435..51207)
                     /locus_tag="AC16O9_11325"
     CDS             complement(49435..51207)
                     /locus_tag="AC16O9_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HIR6517245.1"
                     /translation="MCGIAGELTFDGRLASAASLEAMTDSLAPRGPDGAGLLVRGRIG
                     LGQRRLKIIDLSERAEQPMTDPVLGLSVVFNGCIYNYKELRKELQGLGYVFFSDGDTE
                     VVLKAFHAWRERAPERLQGMFAFAVAERDSGRVTLARDRLGIKPLYYAETKGRVRFAS
                     TLPALLAAGDVDTSIDPVALNHYMSFHAVVPPPRTILKGVRKLPPATVRSIEPDGKAH
                     ERVYWSLSFERSSADIQKSAADWGAELLAALRLAVERRRIADVPVGVLLSGGVDSSLI
                     TGLLAEAGHVDLRTFSIGFEGAHGESGDEFQWSDIVAQRFETEHQRIFIQSSRLLDAL
                     PATVLAMSEPMVSYDNVAFYLLSQEVARHVKVVQSGQGADEVFGGYHWYPKLVGSGDP
                     AQDYARAFMDRDFAAYSSQVSAEYVGLDHARELVQERMAASDAGDPVDRALHLDTTVM
                     LVDDPVKRVDNMTMAAGLEARVPFLDHELVELAARIPPHHKLRDGGKGILKDVARQVI
                     PPAVIDRPKGYFPVPALKYLEGPFLDLAREALGARAAKERGLFRRDYLEQLFADPKAH
                     ITPLQGSELWQAALLELWLQAHGV"
     gene            51318..51755
                     /locus_tag="AC16O9_11330"
     CDS             51318..51755
                     /locus_tag="AC16O9_11330"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6517246.1"
                     /translation="MHAVDRHSKTVGKASGLTLPQLVVLAAIRARGEVTSARLSASVS
                     LTPATVTVILDNLEARGLIERYRNAADRRVVHSRLTPAGADVLARAPGFLHERFVRRF
                     AQLPAVRQEVVLAALEEVAAMMGAPEAAPAPVPLEAGLAAELP"
     gene            51765..53006
                     /locus_tag="AC16O9_11335"
     CDS             51765..53006
                     /locus_tag="AC16O9_11335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517247.1"
                     /translation="MAKRFFYGWRMVGAGAGIQFLQASLLLQSFGAYVAVLQDERGWS
                     KTELSFAAALHQMEAAILGPLLGWIIDRFGPQGVIRTGVVVLGAGFMLLSQIDSLAAF
                     YGAFIVVALGSSMCGFMPLNISLIYWFERQRARALSTLGFGLALGGVAVPLVAWCMAA
                     WGWRVTAFASGVLILAVGLPLAFVMRGRPARYGETVDGEPPLATPSQLPALDTAKAAR
                     DFTAREALRTSAFWLLSLGHGFALFVVTAVNVHAITHLKEGLGYPVQTAALFIMVMTF
                     AQIGGIALGAAFGDRFDKRLVCAGCMILHMTGLLFLTYANGAAMVLAFAVLHGTAWGL
                     RGPFMQAIRAEYFGVSAIGMILGLSFMIIVVGQVGGPLIAGGLADLTGNYRAGFTVLA
                     LLAGVGSLFFLLSKKPARPRG"
     gene            complement(53003..53869)
                     /locus_tag="AC16O9_11340"
     CDS             complement(53003..53869)
                     /locus_tag="AC16O9_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR6517248.1"
                     /translation="MTIRTLQKVIPSIPASDGAGVKLRRSLGASPLARHDPFLMLDEF
                     YSDDPNDYLAGFPSHPHRGFETVTYMLDGHMQHKDSGGNTGDLGPGDVQWMTAARGLI
                     HSEMPQQSEGRMRGFQLWLNLPAKEKMQPPAYRDIAAREIPSVAIERGAVKVIAGEFG
                     GTRGAVHGGTTEPHYWDVHLEPGATFEGALPAEHAAFVYAYEGEAFVGDDRKPLPHRA
                     AGLLSTGDRLSMVAGDTGARVLVLAARPIGEPVVQYGPFVMNSREEIEQAIADYQAGE
                     FARAPVRRLKAA"
     gene            53960..55069
                     /gene="dinB"
                     /locus_tag="AC16O9_11345"
     CDS             53960..55069
                     /gene="dinB"
                     /locus_tag="AC16O9_11345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR6517249.1"
                     /translation="MPATRKIIHLDMDAFYASVEQRDDPTLKGKPVAVGGSPQGRGVV
                     AACSYEARKFGIHSAMPMARAVRACPGLVIVRPDFSKYKAVSQQVMAILRSVTPLVEP
                     LSLDEAYLDVTENALGQPLAMEVARHLKARIFLETALTASAGVAPNKFLAKIASGWNK
                     PNGLTVIAPERVEAFLQQLPVEALWGVGPVTAKKLRALGIGRLVDVRSADEARLRRAV
                     GSHADWLQRLSRGDDPRPVTPNRPWKSISCEDTYPEDLSELREMRAEIERLAKRTASS
                     LEKKGLAARTVTIKVRYADFTTVTRSHTEAPATNDAARLVERARILLERTDAGKRPVR
                     LLGVGVHGLAAAGGASTPGPLPLEAKGPLSPADLL"
     gene            55125..56060
                     /locus_tag="AC16O9_11350"
     CDS             55125..56060
                     /locus_tag="AC16O9_11350"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR6517250.1"
                     /translation="MDPSKLSQFEIVARQDFSDVTYLLEVRHPLMAKAARPGQFVIVM
                     AHEHGERIPLTIADFDRAKGTITLVIQAVGKTTREMQQSCVAGTTLHALVGPMGIPSP
                     VGSAKKVVCVGGGLGVAPIFPQARGLKEAGAYVIGVLGFRTKSLVFWEHKFRACCDEL
                     ILCTDDGSAGLKGLVTEGIRRAIDKHGDIDEVIAIGPPVMMKGCAEATRAHKIKTLVS
                     LNPIMVDGTGMCGGCRVKIGDQVKFACVDGPDFDGHKVDFDDLMTRLRRYTQVERAAA
                     ERWSANCRMKDMADPAAPAVELLELPDPFDDVKGG"
     gene            56062..57555
                     /gene="gltA"
                     /locus_tag="AC16O9_11355"
     CDS             56062..57555
                     /gene="gltA"
                     /locus_tag="AC16O9_11355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR6517251.1"
                     /translation="MPAAKKKTIRTIPQERTPMREQEARARAKNFDEVACGYSVDEAL
                     REAERCLLCPDEPCVKGCPVSIDIPGFIRKIGEKNYRGAYDVIAQTNLLAAVCGRVCP
                     QETQCEEVCTVGESLEPVAIGRLERFVGDAAIREGWVNVPPIEPNGFRIGIVGSGPAG
                     MACAADMAKAGCEVRVFEAFHQPGGVLKYGIPDFRLPNEVVDAEIENLRKIGVRFECN
                     TLVGRLFTIEQMVDELGFHAVFIGTGAGYPSMLGVPGDSLNGVLSANELLTRCNLMRA
                     RDADFDTPIPLGKRVAVIGAGNTAMDAMRVCLRLGAEKVYCVYRRSRAECPARAEEVH
                     HAEQEGIEFHWLTAPVAILDDGKGGVRGMRCIRMELGAPDDSGRRRPVPVAGSEFDFE
                     ADQVVYAIGTNANQIIGQTSRLKLDKRGYIATDEDLATSMPGVYAGGDIVTGAATVIE
                     AMGAGRKAARNMKAYLGIRDPARAAAGETLFGIDRAERNFVRLRAAA"
     gene            57563..59440
                     /locus_tag="AC16O9_11360"
     CDS             57563..59440
                     /locus_tag="AC16O9_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6517252.1"
                     /translation="MPTAQKAVTTLKEHIVEIISDSGEGAQRCGQSLGAIAARMGNGI
                     WTVEIIPAEIRPPARSIAGASGNRIRIGAQRVTNGGDETDLVVAFNEQVLLGRVRAGE
                     LKPGCIILLENMWRSHGDAAIAAAYVETHDRLVEAGYRVIEIPMEQECRTLVSDPRRG
                     KNMFALGMLCHLYSLDLQLAREQVALAFGKKEGSVVAQNVKLLEAGHAWAEANLEFRY
                     RIPAAPVTTPQVVINGNTAIALGVLASGMEICAMYPITPATSASHYLSDVFEKVGGVV
                     HQAEDEIAACAFAIGASYAGKCTVTITSGPGYSLKQEAIGLAVMGEIPLVVVNVQRGG
                     PSTGQPTKVEQGDLLTAIYGSHGDAPKVVMAPSTIEDCFYSIITARRIAETFNMVVVV
                     LSDASLATSQQPFARPEFSESWVAPPVDQSAIPEGMKPYDWNEATGLARRFIPGQPNG
                     MHTLTGLAHDRASHVAYDPEINEESIHFRSLKLAALQKTLKTPPVFGGDSGELLVVGW
                     GSTQGAIEEAVAALREEGRKVSSLHLRFLQPLPGGLKQIMQRFRRVMTIEGNWSDRPD
                     DAIIDEDNRRYSALALLLRARTLVDVDCWSEVKGQPIKPSTIRRVLLDALKPKVKERA
                     R"
     gene            59437..60399
                     /locus_tag="AC16O9_11365"
     CDS             59437..60399
                     /locus_tag="AC16O9_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6517253.1"
                     /translation="MTMMATECLLQLCEQRYEIEDFQSAVPRWCTGCGDNAILAAVQR
                     LCRDEGLRPEKTVFVSGIGCSSRFPHYMRTYGFHGIHGRALPVAEGVRLARPDLTVFV
                     NTGDGDCCSIGAAHWIHAIRYNMNMTVFLHDNQIYGLTKMQASPTSPRGTKSNTTPRG
                     STLEALQPLSVTLGVQNVSFVAQAVDWIPESLYEVVKAAYHHQGFSFVRIVQRCPEWL
                     PKVWDPWLHDPKRILMLDHPQGLRVSDGLKQVYRSTDAHDPSNIDRAREIASDTDRIP
                     VGILYRNPEVPCYEELRATPGLRTIANLKQGLEAELDKFTFWPQ"
     gene            60410..>60690
                     /locus_tag="AC16O9_11370"
     CDS             60410..>60690
                     /locus_tag="AC16O9_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517254.1"
                     /translation="MHAEVHPDAAFYVTGHQAAAAPGTPAAPALRPALFAAYRDLTRL
                     RYDFPLVLRADAADGRVYEPLTALVERLIEGGAERAKALRIEDELRAAAA"
BASE COUNT         9101 a        21522 c        20813 g         9254 t
ORIGIN      
        1 gccggcacct gctcgctctg gtccggatgg acgatgaaac agatttcgta atgccgcatg
       61 ctgtgattct ccttatgggt tgagccatcc actcatgcgc aggtggtatg gcaaggaagg
      121 gggcgaatac tacaggaaaa tctaaggctt gggcagcctc cggcgcactg attcaaagag
      181 gcatacgcct gcagccaccg acacgttcag gctgccgacc gtccccgcca tcggtacgcg
      241 cacgagcaaa tcgcacttct cccgcgtcag acgccgcatg ccctcgcctt cggcccccag
      301 cacccaggcg atcgcttcgg gcaggttggc ctggtagagc gactgctcgg cgcgctcatc
      361 cgtcccgacc acccagatct cgcgctcttt cagctcgtcg atagtgcgcg caaggttggt
      421 gaccatcaga taaggcgttg cctcggccgc cccgctcgcc accttggaca ccgcggcgct
      481 gattcccgcc gcccggtcct tgggcgccac tacggcgtgc acgccggcgg cgtttgccac
      541 ccgcaggcac gcgcccaggt tgtgggggtc ggtcacccca tccaataaca atagcagcgg
      601 cggcccctgc accgcgtcca ggatctcgtc caagctgtgg ctgaggctct tcacctccac
      661 ccgcgccacc acgccctggt gccgcccacc tccgtagaag ccgtccagac gcttggtcgg
      721 caccttcagc accttgaccc cggcgcgctc ggcgaccgtg gcgacatcgc ggatgcgcgc
      781 gtcgtggcgc gtctcgtcca cgtagagttc gagcaccgac gaaggatcgg cgcgcaggcg
      841 cgccaacacc gcatggaacc cgaacaccac ccgcggctcg cgtccgatct gacgactcat
      901 gctcccgaac ccttcctcag ctgctcccaa agctgccgag gtgtcaggat cctcatcgag
      961 ccgaccgcct ccaggtcgag aagctgcttg tcaccggtaa cgaatatctg ggcgcccgag
     1021 gcgagcgcct caccaaggat gcggcggtca tcgcggtctg catcgcagtc cgcaggcgcc
     1081 gattcttcga cgacgagcga agcattgcgc acgatggtct cgatcgcctc cgcgcatcgc
     1141 tcagcaggcg ccttgaactt ctccgcgagc gtccgcgcaa actcgcggag cacctcccga
     1201 ccggtgagca aatcatgctc cagcgcaacc gcttgcagca actcggcact cagcccccgg
     1261 gtcgcgaacg cgctcaccag cacgttcgtg tccatgaaga ccttcacgag atctcgcgaa
     1321 agacatcctc atcggtaaga aagccctgcg cttccgcgta gggcaggatc ttcgcccgca
     1381 cttcgcgaaa gcgcacgatc gccagctggc gcctgagcgc gtccctggcc agctcgctct
     1441 tggtccgccc cgtgcgcctg gccatccgcc cgagctcctt gtcgagcttg gggtcgagcc
     1501 ggattgtcag cgtgctcatc gtcgctcctg taatacagtg tcttacagtc tatcacgccg
     1561 gcacgaaatc gatcttgcgg gaatccaggt ccacgcgcac cagcttcacc ttcatgcggt
     1621 cggccaggcg gtagcgcttc ttcgtgcggc tgccgagcag catgtgccgc gcggcgtcga
     1681 actcgaagta gtcgcgcccg agctccgaga tgtgcaccag gccgtcgacg aagtactcgt
     1741 cgagggtcac gaacaggcca aagggcgtca cgccggtgat cgcgccttcg aacaccccgc
     1801 cgacatggtc ctgcatgtag tggcacttga gccagctctc gacgtcgcgg ctcgcgtcgt
     1861 ccgcgcgccg ctcggtctcc gagcagtgcc gcccgagctc gtcccagtcc acgccctcgt
     1921 agcgccggtt ggcgagcgcc gccttgatcg cgcggtgcgt cagaagatcc ggatagcgcc
     1981 ggatcggcga cgtgaaatgc acgtaggcgt cgaacgccag gccgaagtgg ccgacgttct
     2041 ccggcgtgta caccgcctgc ttgagcgacc gcagcaggat ggtctgcagc agcgcgaagt
     2101 ccggccgcgc cttgatgcgc tcgaggagct gcgcgtagtc cttgggcctg ggcttgtcgc
     2161 cgcccggcag ctgcaggccg agctcggcga ggaactcgcg caagccccgc accttttcct
     2221 cggccggcac ctcgtgcacg cggtacagcg ccggctgccc gcgcgcggcg aggaattcgc
     2281 cggcgcagac gttcgccgcc agcatgcact cctcgatcag ccggtgcgcg tcgttgcgcg
     2341 gctcggcgac gatgcgcgcg atcttgccgt gcgcgtcgaa ctccatcttc gtctccagcg
     2401 tctcgaagtc gatggcgccg cgctgcacgc gcgcggcgtg cagcaccttg aacagctcat
     2461 agagcacggc gaggttctgg ggcgccttgc ccccggacag cctcgcccac accgcggtgt
     2521 aggtgagccg cgcgtgcgag cggaacagcg ccggatagaa ctcgtagcgc gccaccttgc
     2581 ccgcgcccgt gatcgccatc tcgcacacca tcgccaggcg atcgacttcc gggttgagcg
     2641 agcacaagcc gttggagagc ttctccggca gcatcggcag cacgcggcgc gggaagtaga
     2701 ccgaggtgcc gcgtgcgcgc gcgtcctggt cgagcgcttc gccgtcgcgc acgtagtggc
     2761 tgacgtcggc gatcgccacc cacaggcggt agcccttgcc ctctttttcc gcgtagaccg
     2821 cgtcgtcgaa gtcgcgcgcc gtttcgccgt cgatggtgac gaagccaagc ctcctcaggt
     2881 cgcggcgccc ccgcgcatcc tcctcgcgca ccgcctccgg cagcgcgcgc gcctgcgctt
     2941 cggcgcgctt cgagaattcg tgcggcagat cgaacttgcg cagggcgatc tcgatctcca
     3001 tgcccgggtc ggcgtaattg ccgagcacct cggcgacgcg gccgatcggc tgcgcggttt
     3061 tcgaaggctg cgtcaccagc tccaccgtca ccacctggcc ggccttggcg cgcccggcat
     3121 cggccggcgg caccaggatg tcctgcgcga tgcgccggtc ctcgggcacg aggaaggtca
     3181 cgccgcgctc gctgtgcagg cggccgacga cgcgccggtt gacgcgctcc agcacttcga
     3241 tgatctcgcc cgagggccgg tcgcggtgat cggcgcccgc gacgcgcacc acggcgcggt
     3301 cgccgtgcat cacctggcgc atttccgcgg ccgagagaaa gatgcgcggc tcgccgccgt
     3361 cgggtgcgag aaagccgtgc ccgtcggcgt ggccctcgac ccggccgctg aacttgccga
     3421 gcgccagcgg cgcggcgcga gcgcgctcgg tgcgatgggt gcgctcttca gcgcgtcgtt
     3481 tctttgacac gaaaatcctc agacactaga atgcgcctcc cctgccgagg tggcggaatt
     3541 ggtagacgca ctaggttcag gtcctagcgg tggcaacact gtgggggttc gaatcccttc
     3601 ctcggcacca cgctcaagca aacggatggc gcctgacgat ggtctcgtcg cgctccgggc
     3661 ccgtcgagat gagatcgatc ggcacgccgg cgagcccctc gatgcgcttc aggtagtcgc
     3721 gcgccgcctg cggtagcgcg tcgtacttct tgatgccgac ggtgctctcc ttccatccag
     3781 gaagctcctc gtagaccggc tcgcagcgct cgagctcctc ggcgcccacc ggcaggatct
     3841 ccgagagctc gccctcgatg cggtagccga cgcagatctt cactgcctcg acgccgtcga
     3901 gcacgtcgag cttggtgatg cacaaccccg agaccccgtt caactggatc gagcgcttca
     3961 gcgccgcggc gtcgaaccag ccggtgcggc gcggccgccc ggtggtggcg ccgaactcct
     4021 gcccgcgccg gcgcaactgc tcgccgacgt catccgagag ctcggtcggg aacgggccgc
     4081 tgcctacgcg cgtggtatag gccttggtga tgcccaggac gtagtgcagg tggtgcgggc
     4141 cgatgcccgc accggccgcc gcggcacccg aaacgcaatt ggaggacgtg acgtacggat
     4201 aggtgccgtg gtcgatgtcg agaagcgacc cctgcgcgcc ctcgaacagc aggttcttgc
     4261 cggcgcggtt ggcatggtag agcgcacgcg gcacgtcggc gaccagcggc gccaggcgcg
     4321 gcgcgagcgc cagcgcctca tcgagcgtct tctggaactc caccgcgggc tggccgtggt
     4381 actgcgtgag gacgaaattg tggtactcga gcagcccctg cagcttctcg gcgaagcgca
     4441 cgggcttgaa caggtcctgc aggcggatgg cgcgccgcgc caccttgtcc tcgtaggccg
     4501 ggccgatgcc gcggccggtg gtgccgatct tctgcgcgcc gcgcgccgct tcgcgcgccg
     4561 cgtcgagcgc ggcgtggtaa ggcatgatca gcgggcaggc ctccgaaatg cacagccgcg
     4621 ccaccacgcc gcgcacgccg gcggcttcca gctcatccat ctcctggatc agtgcctgcg
     4681 gcgacaccac cacgccgttg ccgatgaagc agtccacgtt ctggcgcagg atgccggacg
     4741 gaatcaggtg cagcacggtc ttcttcgtgc cgatcaccag cgtgtggccg gcgttgtgcc
     4801 cgccctggaa gcgcaccacg ccctgcgcgt ggtcggtgag ccagtcgacg atcttgccct
     4861 tgccctcgtc accccactgc gtgccgatga cgacgacgtt gcgcgccgct ttgctcactt
     4921 gcgcccgccc ttgcggccgt tggcggaggc gagcgcccgc aggtcgatgg aaaagccggt
     4981 cgccggccgc gcgcggccga aggccttccc cacctcgtcg tagcgcccgc cgcgcgccac
     5041 cgcgccgctg acaccatcgc aataggcgtc gaacaccacg cccgaatggt agtggtagcc
     5101 gcgcagctcg gcgaggtcga agctcgccgg caccgggcac gccttggcga gcgcgcgcag
     5161 cgtggcgagc gcgcgcttca ccggcgcgat cgcgggcagc ttcttctcgg cgcgcgccag
     5221 cacctcggcg ccgccgtaga gctcgggcaa cagcagcagc gcctcgcgcg ccttcgagtc
     5281 cagcccgcgc gtcaggccgc gcagcgccgg caggtccttc ttctgcagcg cctcgaacag
     5341 cgccgcctcg agcgtcgcgc ccgccccggc agcctgcacg atggcgcgga acaccgccac
     5401 gtggccgatg tccaggcgca gcttcttcac gcgcgccagc gccagcgcgc ggcacagcag
     5461 ccgctgcact tccaggtcgc tttccacgcc ggcgtggccg tagacctcgg cgccgatctg
     5521 gatcggctcg cgcgtcgccg ccggtccggc gggccgcgtg tgcagcacgc tgccgcagta
     5581 gcacaggcgc gtcacgccct tgcgattgag caggtgcgcg tcgatgcgcg ccacctgggg
     5641 cgtgatgtcg gccctgaggc ccatcatgcg ccccgagagc tggtcaacca ctttgaaggt
     5701 gcgcacgtcg aggtcgtgcc cggtgccggt gaggagcgaa tccacgtact cgatgagcgg
     5761 cggcatcacc agctggtagc gcgccgcggc gaacaggtcg aggatcgcgc ggcgcagccg
     5821 ctcgatgcgc gcggcttccg ccggcaaaat gtcctcgatg tactcgggca ggagccagcg
     5881 catcacgtgg cgagggcgag aagcagcagc ccgatcagca tcgaggtgag gccgaagaag
     5941 cggatctgtc cgtcggagag ctgcgtgacg cgccggaacg tgtcgcgcca gaggctaggc
     6001 gcgaggaagg ggagcacccc ttcgatgatc agcatcaggg cgaaggcaag gaggaacgtg
     6061 gtcgccatgg cgcgtcattt cttgcccccg gccctgccgc cgggatcctt gagatagcgg
     6121 aagaaatccg agctcggctc gagcagcagc aggtcggcgc ggccgcggaa cgtcgagcgg
     6181 taggcctcca tgctgcggta gaacgaatag aactccgcgt cgcggctgaa agcctgcgcg
     6241 taggtcgcgg cggcgcgcgc gtcgccgtcg cccttgacgc gctgggcgtc gcgataggcc
     6301 tcggcaagga tcacctcgcg ctggcggtcg gcatcggcgc ggatgcgctc ggattccgcc
     6361 gcgccggtcg aacgcaactc gttggcgatg cgctttctct cggcctccat gcggcggtac
     6421 accgactcgc tcacttcctg aggcaggtcg acgcgcttca ggcgcacgtc gacgatctcg
     6481 acgccgatgc ggcgcaggtc ctggtccgcc ttttccctta cccgtcccat caccgcgtcg
     6541 cgctcgccgg agaccacgtc atgcacggtg cgcgcgccga attcgtcgcg cagcgccccg
     6601 gagatcgtct ggcgcaggcg ccgcgtcgcc tccagctcgt cgccccgcac cgagacgaag
     6661 tactgcttga cgtcgacgat gcgccagcgg atgaacgaat ccaccagcac cggcctgttg
     6721 ccctgcgtga ggaagcgcag cggctcggca tcgtcatagg cgaggatgcg catgtcgaac
     6781 aggcgcacct tctggatcag cggccacttg aaatgcaggc ccggcttggt gatgacgtcc
     6841 ttcacctcct cgaactggaa cacgatcgcg ttctggcgct ggtcgaccgt gaacatcacg
     6901 ctggaggcga ccagcagcag cgcgaccagg acggcaatga ccggcccgaa ggctctcatg
     6961 tcagcgctcc ccccgctcgc ggttgcgcag gctgtcgcgc gagcgcggac caggatccat
     7021 cggcagcgcg gcatccgacg aacgctgcga ggcctgcgac tcggtcagcc cggaaacccc
     7081 cgactgctgc atcagcttgt cgagcggcag gtacaggagg ttcccgctcg cccgggtatc
     7141 gaccaggatc ttcgaggtcg acgacagcac gctctgcagg gtctcgatat agatgcgctc
     7201 gcgcgtgatc gccggcgcct tggagtattc ggtcagcacc tggcggaagc gcgacgcctc
     7261 gccctcggcg ttggccacca cccgcgcctt gtagccctcg gcctcctgcg tgagccgcgc
     7321 ggccgcgccg cgcgcccgcg ggatgacgtc gttggcgtag gcctggccct cgttcttctg
     7381 gcgctcgcgg tcctgcccgg ccttgaccgc gtcgtcgaat gccgcctgca cctgctcggg
     7441 cggctgggcg ttctggatgg tcacgcggct gaggctgatc ccggtcttgt agttgtcgag
     7501 gatctgctgc atcagcacct gagcctggtt ggcaatgttg tcctgcccct cgtacaggat
     7561 ggcgtccatc ttgctcttgc cgatgatctc gcgcatcgcc gcctcggcgg tcagcatcgc
     7621 cacttcctcg ggccggcggt tgttgaacag ccaggcctgc gcgtcggtga ccacgtactg
     7681 cacggcgaac tgcagatcga cgatgttctc gtcgtcggtc agcatcaaag actccttcag
     7741 caccttggtc ttgacgctgt tgcggtagcc gacctcgagc gtgcgcacct gcgacaggtt
     7801 cacgacctcg tgcgactgga tcgggaacgg catgcgccag cgcaggcccg gatcggtgat
     7861 ctcggagaac ttgccgaagg tgagaaccac gccgcgcgac gcggcatcca ccacgtagaa
     7921 gccgctcgcc agccagatca ccaccaccag cgcgaccagc acgccggcgc cgccgccgag
     7981 catgcggccc gagggcggca tggggggaat gtcgccaccg ccgccgcggc gcccgccgaa
     8041 caggtcgttc agcttgcggt tgaaattgcg ccacagctcg tccagatcgg gcgggccttg
     8101 gttgccgccc gggccgcggg agccgcgccc ccagttcgga tcgttcaatg acattacagt
     8161 gagatcggtt ccggagaagg ggtgcggggt tgccgcgctc tggcggcctc ggcaatcgcg
     8221 tcccgcagat cggcgaggcc ggcgccgctg cgcgcgctaa cgaacacgcg gccaatccta
     8281 ccatactcgt cgcgctcgac gccgggctgc aagccggaca ggtcgatctt gttccatacc
     8341 aggatctgcg ggatgccgcc ggcgtcgatt tcctcgagca ccttgtcgac ctcgagcgcc
     8401 tgctgctcgc gcaccggcga ggcgctgtcg acgacgtgca gcagcaggtc ggcgtgcacc
     8461 gtctcctcca gcgtggcgcg gaacgccgcc accaggccgt gcggcaggtc gcggatgaag
     8521 cccaccgtgt cggagacgac gaccgtcccc gcctccggca gccagatgcg gcgcgtggtg
     8581 gtatcgagcg tcgcgaacag ctggtccgcc tcgagcgccc gggcgtgcac cagcgcattg
     8641 aacagcgtcg acttcccggc gttggtatag ccgaccaggg acaccgacag cacgtcgcgc
     8701 cgcgcgcgcg cgcggcgctg gaccacgcgg cggtcgcgga actgctccag cttgcccttc
     8761 agcatgcgca cgcggtccgc gatcatgcgc cggtcgagct cgatctgctt ctcgccgggg
     8821 ccgccggtct tgcccagccc gccgcgctgg cgctccaggt gggtccagcc gcgcaccagc
     8881 cgtgtcgaca ggtgctgcag ccgcgccagc tccacctgca gcttgccctc gtgggtcttc
     8941 gcccgctgcg cgaaaatggt aaggatcagc tcggtgcggt cgagcacctc ccgctcgagc
     9001 gctttctcga ggttgcgttg ctgcgccggc gacaaggcat ggttgaagat cactgtcgcg
     9061 gcgttcaaac cctgcgccgc gcgcgcgatc tcctgcacct tgccgctgcc gacgaagaac
     9121 ttggggtcgg gtcgctggcg ccggccgcgc acctcggcat ggcgccggat gccggcactg
     9181 tgcgcgagcc gcagcagctc ttcggccgcg tcctcggggg ccgccccgcc gaggtccagg
     9241 ctcacgagca ccgcggcctg gttgtcgggc gcggccgcgg aaatcaagcg tccttttcgg
     9301 agtcggcgcc gaggctgatg gcgcgcgacg gcacgaccgt ggaaatggcg tgcttgtaga
     9361 ccatctgtgt caccgagttg cgcagcagca ccacgtactg gtcgaacgat tcgatttgtc
     9421 cctgcagctt gatgccgttc acgaggtaga tcgagaccgg gacgtgctcc ttgcgcaggg
     9481 tgttcaggaa cgggtcttgt agcagctgcc ctttgctact catgttattg cctcactcgc
     9541 gtggggtgga gcaatactgt aatggattcc aaccggcttt tctataccgt gcgcgtcggc
     9601 ggcgtcacgt cgccgcattg tgcgcgatgc cggagcgcgt gatccatcag cacgcacgcg
     9661 agcatcgctt cggcgatcgg cgtggcgcgg atgccgacgc agggatcgtg gcggccgctg
     9721 gtcgacaccg tggcgggcgc gccgtgcacg tcgatcgtgc gccgcggcag gcggatgctc
     9781 gaggtcggct tgagggcaat cgacaccagc acgtcctggc cggtggagat gccgccgagc
     9841 acgccgccat tgttattggt gaggaagccc tgcggggtca tctcgtcgcc gtgctcgctg
     9901 cccttttgcg ccaccgcggc gaacccggcg ccgatctcga cccccttgac ggcgttgatc
     9961 gacatcatgg cggccgcgat gtcggcgtcg agccgcccgt agaccggctc gccccagccc
    10021 accggcacct tgtgcgccac gacgttgacg cgcgcaccgc aggaatcgcc gctctcgcgc
    10081 agctggtcca tgaatttctc cagctcggcg acggcgccgg catcgggcgc gaagaacggg
    10141 ttggcgctca cggcgtccca gtcgcgaaac gcgatgcgat gcggcccgag ctgcgccagg
    10201 tagccgcgga cctgcacgcc gtagcgcacg ccgagccact tcctggcgat ggcgccggcg
    10261 gccacgcgca ccaccgtctc gcgcgccgag gcgcggccgc cgccgcgcgg gtcgcgcacg
    10321 ccgtactttt gccagtacgt gtagtccgcg tgacccggac ggaacttctc cgcgatcgcg
    10381 gcatagtcct tggagcgcgc gtcctcgttc cttaccagga agccgatcgg cgtgccggtg
    10441 gtcctgcctt cgtacagccc ggacaggatc tctaccgtat ctgtttcgcg gcgctgcgtg
    10501 acgtggcgcg agctgccggg ctttctccgg tcgagctctt gctgcaggtc cgccgccgaa
    10561 agctcgagcc ccggcgggca gccgtcgacc acgccgccgt aggccgggcc atgcgattcg
    10621 ccgaacgagg tcacgcaata cagcgtgccg aaactgttgc cggacatgcg gaagattcta
    10681 cacggcgcct tcgaagaagc gccgcgtctc gaacgcacgg gcggcgaagg cgctgccgtt
    10741 gcgccgcagg ctgcgcttga gcacgaaatc gaacaacatc gggttgtgcg cacggatctc
    10801 ggtgagcacg ggcgccgcgc tcgcggcgag caggccgagc gcgaccaggt aatcgaccga
    10861 gaagatcggc atgatcccgg gcagcggata gtcggagcca ttgagcagcc gcgcgtgcca
    10921 gtcggtgcgc tcgagcagcc gcaccagcgt gccgccggcg cggttcacct gcgtgatcgc
    10981 agatatgtcg ccgtaaacgc gcccggcaaa gcgcgtctcg tccatcaggc ggccaaagag
    11041 cgcgaagctg tcgacccacg gcccgcccgg tccccggtcg aggtcgcggt cctcgccgag
    11101 cgaagcgcag tgcgccacca cgacacgcac gccggcgtcc agcgcgcggc gcagtttcag
    11161 cggattgccg tgatcctgcg cgtccgaacc gacgacggcg agctcgcgtc ccgcatgcga
    11221 gataagcggc agctcgaggc gcgcggccgc cgcatagaag cgctcgcaga gcgccgaagc
    11281 cgggtccatg cccatcgccg ccggcagcca ctttacggcg cgcgcgccat cggccttcgc
    11341 gcgctccagc gcctccacgc agtcggcgcg gtaggggtgg atcgaggccg cccattcgaa
    11401 tacctgcggg tgcgcgcgcg cggtatcccg cgcgtaggcg tccggcacgt agaacccggt
    11461 gcgctcccac tgcacccggc cgcgctcgtc gtgcgcgcgg tcgaacgcga acagcagaag
    11521 cttggtgccg gcgcgcatcc cggccatcag gttgagcatg cgatcgacgt acgcgcgatc
    11581 gacgctgccg ggcgcatcgt gcacgcagcc ggcgttgagg aagaacaggc gccgcgcgta
    11641 ctcgcccgga ttcagcaggc tctccatgcg cggattgacc accacgccgc tgcccgcatc
    11701 gccggtgccg acgaggtgcg catgcgcgtc ccaaacctgc gcggcgtcga tgccacccca
    11761 agcggcgcgc accagctcgt gctcggcgag gcggcgcggc agccgcgaaa ggcaaggatt
    11821 ccacaggccc tgctcgggcc accagcgcca gacagcgccg ccggccgcgg ccagggtcag
    11881 cgcggacgcg ccgacgagaa acttcctacg cgggccaatt cttgatgtac cccttgagca
    11941 tcttgttctc gaaatcctgc tcctcgagca ccgccttggc gacgtcgcgg aacgagatca
    12001 cgccgaggag cttgccgtcc tgcaccacgg gcaggtagcg cgcgcccgac tcgagcatcg
    12061 tgcggcgcag gtcgttgact tccatgtcgg gcgtggcggt cacgggatcg cgcaccatga
    12121 cctcggcaat gcgcagctca gcgagggtgc cactgcgctg cgccagcgcc tgcaggattt
    12181 cccggaatgt cagcacgccg accatgcgcc cctgctccag cacgatgagc gagccgaggt
    12241 tctgctccgc catcgtcttc accgcatccg tcgctcgtcc cgacggctcg atggtcaaca
    12301 gccggttgcc cttgacgcgc aggatctcct tgacttgcat gtcggcctcc catgcctgct
    12361 ccgctcagcg cattatagta gggacagtcc ccatttttcg gtcgcgccgc ttggggacat
    12421 gggggcagtc gctagctcac cagcagcgcc gcaaggccaa gcagcatgcc catgctcacc
    12481 acgtcggtcg ccgtggtgag gaagatgctc gaagcggtcg ccgggtcgaa gcccaggcgc
    12541 ttcagggtga gcggcaccat ggccccggag agcccgctcg ccacgcacga gccgaccatc
    12601 gccatgaaga cgacgaagcc gagcaggacc gggtgcgccg agttctggct gctcgcggta
    12661 atgtacatgc cgatcgccgc caccaagcca acgcccgcgc cgttcagcag gccgaggccc
    12721 gcttccttgg tgatcagcgc gcgctcggag ccgggcttga gctcgccgat ggtcatgccg
    12781 cgcagcgtca cggcgagcgc ctggcagccc gtgttgccgg actgcccggc gaggaccggc
    12841 aggaacagcg cgaggatcac caggcgatcg atcgtgtcct ggaaaatgcc gacgaccgcg
    12901 gcggcgagga atgccgtaag caggttaagc tgcagccagg ggtggcgcat catcaggctc
    12961 cgctgccacg gcgtgcccat gcgctcctcc ttttccacgc cgaccatgct gccgggctgt
    13021 gccgcgatct cgaaggcacg cgcctcgaac atgctctggc cgcgcaccag gccgcggatc
    13081 cggttctcgg cgtcgaccac cgggtacacg gggtagtgcc ggtcgagcac ctgcttcatc
    13141 gcttcgtcga gcaccgtcgg cgcgtgcagc gcgaacacgt cggtgagcat gacgtcgcgc
    13201 agcgcccgcg cgcggtcgtt gaacaggagg tccctcatgg tgacgatgcc gagcagccgc
    13261 tcgtcctcgt cgacgatgta gatgtaggtg atgaaggcat gcttcacgag ctcgtgcagc
    13321 gcctcgatcg cctctcccac ggtggcctgc ggcggaaatg ccgcgtacaa cggctccatc
    13381 atgcggccga tcgtgccttc cgcgtagagc gcgttgcgct gccactggcg cgcaatctcg
    13441 accggcgcgg cggcgagcgc gcgctcgcgc gtatcggcgg gcaggtaggt cagcacgtcc
    13501 tgcgtgaagc cgggcgagag cttgagcagc accgcggcga cctcggcgcc gccgtagtgc
    13561 gcgagctttt cggctgcgcc ctgcggcgcg cgcagcttga cgtcctcggc gaggtcgatg
    13621 gccgagcgct ggatggtggg gcgatcgatc gattccacgg cggactcctt ttccttgggg
    13681 tgcgctacag gccggcggcg tcggcgacca gcttgcgcaa ctcgcgtcgc aggcgcgcct
    13741 tgagcgcgcc gtggcgcgcg ccgcgcgcca ggttcttcag ctcccaggga tcgcgcgcga
    13801 ggtcgtagag ctcgtcgagc tcgcccgcgg cgccacggtt cacccagcgg atcagcttgt
    13861 agcggtcggt gcgcaccgcc ttgtaggtca tgccgacgag ccagggcatg gcctgctccg
    13921 cccagtactc caccaggaac gacttgcgcc agcccttcct gcggccggcg agcagcggca
    13981 gcaaagaccg tccctggacg tgcgcgccgg gcctgccgcc cgccaattcg acgaaggtgg
    14041 gggcgatgtc ctggcagatg acgaggtcgc ggttgcgcgt gccggcccgc gccttgcgcg
    14101 ggtagcgcac gacgaacggc gagcggatgc cctcctcgta ggcgaagcgc cgctcgggcc
    14161 cgaggccatg ctcgccgaag aaatagccgt tgtcgccgag aaacacgatc gccgtgtcgt
    14221 cgagcttctt ctggcgcgcg agcgcgtcga gcagctggcc cacaccctcg tcgaccgaag
    14281 ccatcatgcg cgcgcgctgg cggatctcgt cctgcgagcc ggcctggatc gcctccagca
    14341 gggcgcgcga gcgctcgctg cccttcagct cgaacgcctc ggtccacgcg ggcgcctgtt
    14401 ttaccagctc atgggtcggc cgcatattgg gcgtcttcgg gaacacgccg tccgcgtaca
    14461 gcgcacggtg gcgttcggcc gggatgtagc ccccgaagcg gctcatgtcg agcgtgccgt
    14521 cggccgcctg gtgcgcatcg ggatgcaccg ccttgtgcgc gaagaacagc gcgaacggct
    14581 ttgcacgctt gcgcacgacg aacgcaagcg cctcctggtt caacaggtcg gtcatgtagc
    14641 ccgggcgctg ctcgtagcgg ccgttcacgt tcagcctggg gtcgaccagg ctgccgtgcc
    14701 cgtcgaagct cacccagtgc gcgtagcccg ggcgcggcat gccgtcgttg cccatgtgcc
    14761 acttgccgac gtgcgccgtg tcgtagccga gcttttgcag cgccagatgg tagttcggca
    14821 ggcggtggct catggcatcg cgcgccacgt tgtcgatgat cccgtggcgg ctcgcgtact
    14881 ggccggtgac gatggaggcg cggttcggcg agcagatcgg cgtggtgtgg aacgcgcgct
    14941 cgaacagcac cccttccgcg gccagccggt cgatgtgcgg ggtcttcatg tacgggtggc
    15001 cgccggcgcc gaattcgtcg tagcgcaagt cgtcgatcag gatcacgatc aagttgggct
    15061 tcatgggccg caagtctaag cgcggggggg cgcaagtgcg tatttttccc cggcgcgcag
    15121 gccgggagtt ccgtatgctg ctccttgcat ttcctgacag ggaaggaggc tggggcatgg
    15181 caatgacgca accgcaggtc tcggtgccgc aatggctcgg ccggcagttg ctcgggccgc
    15241 tggacgatgc ggccgggctg gtcaagcgct accgggagct ggacgccttc cgggactacc
    15301 tcgcggcccg ggtacggttg gtcgccccgg cgctcgctct ggtcgtggtg gcggcgctcg
    15361 cttgcgggct ggcgcccatc gtggtgttcc tgagcacgaa cgccgcagcc gcgctggtcg
    15421 gcctgctgct cgcgccggtg atgctgatcg gcagcctgtt cgtgctgagc ctggtgctgt
    15481 tcgcgtggct ggaggaacgc tcgctggccc gggcgctcgg gcaccgtacc ggcccggcgc
    15541 cgggcaggct cgcgcgctgg gtgaagcgca agcttggcgc ggacatcgga agcgcgctgc
    15601 gcgtgccgtg gctgctgggc gcgctgttcg tcgtcgcacc gctggcactc ctcgccacgc
    15661 gagccccggg aacggtcgcg ctgctcgtgg cgctgcttgc gctctgccct atccttttcg
    15721 cgcgcctgga tcgctgagcg gcgcaaggcc gccgagcagc gtcggcacga gctcgctcac
    15781 cgtcgggtgg atgtgcatgg cgcgctgaat cgtcgtgtgg ggccggttcg cgtacatgac
    15841 gtccgggagc acgtgcttca ggcccatgat ccacttggtg acaccgtcga ccgactgctt
    15901 caccaccgcg tccttgcgcc gcttgaccgc ggccatgtcg acgcgcacgt cgccggcgct
    15961 gaagccaaac tcggcgccgc ggcgcgccat gtgcgcggcg cctgcgctcg ccacgagcgt
    16021 cttggtcggg atgcagcccg tgttcacgca ggtgccgccg acgagcttgc gctcgatgac
    16081 cgcggacgtc agcccttcgc ggtcgagccg cgcggcgagc gccggcccgg cctggcctgc
    16141 gccgatgatg attgcgtcga atcgttccgc cgtgtcgcac cctccgtgtg ccctcccact
    16201 atactgccgc gcatgcgcgc cggcgccgcc ctccttgccc tgctcgccgc gcacgtgccc
    16261 gccgcgtcgg cccagtcgtc gccgcgcctg gaacccggcg tgtcactgga gctcgcgcgc
    16321 tggcgcgcgc agcattaccg cgatccggcc tatgcgctgg agctgaagat cgacgcgcgg
    16381 cgccggcgcg ccagcggcac gctcgagctg caggtcacgc ttgcgcgccg cgtcgacctg
    16441 gtgctcgatt ggcgcggcgc gccggtgcgc ggcctgcgcg tcaacggcaa gcccgtgcgt
    16501 gcgcgccagg agcgccagca cctcgtggtg ccgcgcacgg cgctcgtgcg cggccgcaac
    16561 cgcgtcgagc tcgcattcag ggccccgatc gccgtttcgg gccgtgcgct gaccctctac
    16621 cgcgaccgcg aggacggcgc gagctacgtg tactcgctgt tcgtgccggc ggacgcaagc
    16681 agcgtatttc cctgcttcga ccagcccgac ctcaaggcgc gctttcgcct cgcgctcgag
    16741 ctgccgcggg gatggcgcgc gatctcgaac gcgcccctgc tcgaagaggc gccggggcgg
    16801 gtgcgcttcg ccgaaaccga gcccatttcg acctacctgt tcgccttcgc cgccgggccc
    16861 ttcgaggccg tgacgcaagt gggcgagccg gtgcggctgt tcgtgcgccg ctcgcagctc
    16921 gagcgcgcgc gcgagcacgc cggcgaggtg ctgcgcctca accgcctggc gctcgcgttt
    16981 ttcgaggcgg aattcgcgcg ccgcttcccg ttcgccaagt acgacctggt gctcgtgccc
    17041 gagctcgcct acggcggcat ggagcacgcc ggcgccacgt tccttaacga gcagtcgatc
    17101 ctgtttcccg acccgcccag cgggcccgac ctgctgcggc gcgcgcagct catcttccac
    17161 gaggcctcgc accagtggtt cggcaacctc gtcaccatgc gctggttcga cgacctgtgg
    17221 ctgaaggaag gctttgccaa cttcatggcg gccaaggcgg ccgccgcgat cgtcccggag
    17281 ctcgagccct ggagcgcgtt ccacgcgctg aaaaccagcg cctaccgcac ggacgccacc
    17341 gcgggcacca cgccgctgcg ccagccgctc gccaacctgt cgtcggcgaa atccgcctac
    17401 ggctcgatcg tgtattcgaa gggtcccgcg gtgctgcgcc agaccgagtt ctacctcggc
    17461 gagcacgtgt tccggcgcgc gatgcgcgac ttcctgcagc gtcacgccta cggcgccgcg
    17521 gactggagcg acctggtgcg ggccttcgag cgcgtcgagc gccgcgacct gcaggcctgg
    17581 gccgcggcct gggtgcagcg ccgggggcta cccaccgtga cgctgcgcga gacccctggc
    17641 ggcgtgctgc tcgcccagga ggacaccctc ggcgaggacg gcgtgtggcc gcagaagctg
    17701 gtggtggctg cggcgacggc cgagggcagc gtcgtcacct cggaggtacg gctcgagcag
    17761 gcgtccgcca cggtcggcgg ccccgcgggt gcacgcgtgg tgcgctggcg ctaccccaat
    17821 gcgggcgact tcggctacgg gcgcttcctc ctcgacgcgg cgagccgcga cgcgctgctc
    17881 gccgagccgc gcgcgctgcc cgacgggctg ctgcgcgcgc agctcatcga ggcgctgtgg
    17941 gaaagcgtgc gcgacgccga gctcgacccg acggctttca tcgacttcgc gctggtgctg
    18001 cttccggagg aaggcgacga cgtgatcctg gccgggttgc tggcacgcgt ggagggcgcg
    18061 tttcggcgct acctgtcgga agcgcagcgc ggccgcctcg cgccgcggct cgagcgcgca
    18121 ttgctcggcg ctgcgctcgg cgaaggcccc gaaagccgcc gccggctcat gctgcgcgcc
    18181 ttcgtcgcgc tcgcctggtc gggcccggca ctcgccgatc tcaagcgcct ccacgaaggc
    18241 tcgctctccg cgcccggcct cgagctgggc gagcgcgacc gcttccgcat cctcgcgcgg
    18301 ctgctggtgc gcgccgacgc cgacggcccg cgcctgctgg gcgaggccgc cgcggccgag
    18361 cgcggcgata acgcccggcg ctacgccttc gccgcctcag cggcgctggc cgacgccgag
    18421 gcgaagcgcg ccatgtaccg gcgcttcacg cgcgacgccg agctgccgga gcgctggatc
    18481 gaggaggcgc tcgcgtcgtt caacgcgccg gagcatgcgg cgctcaccgc gcctttgctg
    18541 agcgccgcgc tggcgcagct gcccgagctc aagcgccggc gcaagatctt cttcgtcgag
    18601 cactggctgg atgcgttcct cgccggccag accgagccgc gggcgctcgc cgcagtgcgg
    18661 cgcttcctgc gccgtgacct cgacccggac ctgcggcgta aagtgctcga agctgccgac
    18721 gggctggagc gcacggttcg catccgcgaa cgcttcgcag cctcggccga acgatgacaa
    18781 cccggcgcga ttgagattag acttcccgca tgaccgcgca tgccatttcg gtcaggctcg
    18841 ccgggcagcg cgacgcacag gcgatcgcgc tcatgtcgcg cgacctggtg gaggcgggac
    18901 tgggctggaa atacgatgcg gcccgcgtcc tcaaggcgat ccgggatccg gaaacgctga
    18961 cgccggtggc gtgcgaccgc gggcgcatcg cgggctttgc gatcatggag ttcggcgacg
    19021 agcgcgcgca tctcgtcctg ctcgccgtgc ggcccacgca gcggcgcatg ggcatcgggc
    19081 gacgcctggt ggactggctt gtggaatcgg cgcgcacggc cggcatcgcc agcgtgcacc
    19141 tggagctgcg ggcgggcaac gaggcagcgc gcggctttta tcgcgccatg ggtttcgacg
    19201 agaccatcgt cgtcccgggc tactaccggg gccgcgaagc ggccctgcgc atgattcgcg
    19261 tgctgcgctc gaccagcccc gtgccttaca cctggcggcc gtcgaccacc tgagcgcgcg
    19321 cgcctagggc acgatggcga agcccaggcg cagctgcgac ttgaagcgcc ggtcgtagtc
    19381 gagaatgtct tcgccgtagc cggcgaagaa ctgcacgtga aagtagccgg agaccggccc
    19441 gaagccgagc acgcgcgtgc gctcgaacca gtcgagcgtc aggctgccct tgcccgccgt
    19501 gccgaggcgc gccatcgcgc gccagctgcg tacgtcatcg ccatagcgca agcgccagtc
    19561 gacgtatccg cggtagcgct ggatgtcggg gttctcctcc ttgtcgaggt aaccgtagag
    19621 cttcggcgcg agctcgagcc gcttgccgtt gtcgagcagc cagacccact cggggtgcac
    19681 gaacagcatg tcgatggagc gtgactgcgc gccatccttg ccgttcgact cgtgctcgaa
    19741 gccggcgcgc aggccgtcga tccatgtctt gtcgccggcg cgcttccagt cccagaacag
    19801 cgacggccag tagctggtgt cgcgaaacgg cttggagtcg cccgacaggt tccacagcga
    19861 cgtctgcgtg tagccgaggt agaaaccgga gagccagggc tgctcgccgc cccagcccag
    19921 cccgcggtcg aacatccggt acttgaacga cagctggaag cgcgcgttgg tgccgccgcg
    19981 tgtgcccatg acaaagtaca ccggttcgtt ctcggtgagc ggcggctcgt ggccttcccg
    20041 ttcctggttg acgatgaagg tctccatcac atccgggcgt ggctggtcct gcgcggcggc
    20101 gggcagcgcg gcaagcgcgc acagcaggag cggccagcgt tgcatggttc tgccagaatg
    20161 gtcggcgatg acggagtcta gcgcgccgct gcccgcggtc ttcatctcgc acggttcgcc
    20221 gatgcacgcg ctcgaggcgg acgcgagcgc gaccggccgc gcctggcggg cgctcggcgc
    20281 gcggctgccc cgcccgcgcg cgattctcat cgcctcggcg cactgggaaa ccgagcagcc
    20341 ggcgctgacc ggcacggcgc gccccgaaac gatccatgac ttccggggct ttcccccgcc
    20401 cctgtacgag atccgctatc cggccccggg cgcacccgac gtggcggggc gcgcgcaaca
    20461 gctgctcggc gcggcgggcc tggcggcctc gatcgacccg cggcgcggcc ttgatcacgg
    20521 cgcctggacg cccctgcttt atgtctaccc cgccgccgat gtgccggtgg cgcaggtttc
    20581 cgtgcagacg gcgcttgggc cgcgccacca cctggcgctc ggggccgcgc tggcaccgct
    20641 cgcgcgcgag ggcgtcctca tcatcggctc cgggcacctg acgcacaacc tgcgcgaact
    20701 gcgcagcgta cgggagaccg gcgtcgggcg cgccgagccg tatgtggccg aattccagga
    20761 atgggtgcgc gagtgcatcg agacgcacga ctacggtcgg cttgccgact atcgccagct
    20821 gtcgccggcg ggcgtgcgcg cgcacccgag cgaggagcat ttcctgccgc tgttcgtggc
    20881 gcttggcgcg gcgggcggcg aggcgcgcgc cgagcggatc gtcgggcacg tggagggcgg
    20941 cgtgctcgcc atggatgcct atctcctgag cccgggaccg gagaaatcct gacacggggc
    21001 ggtgtgactt agtgcacgct ggccacgttc tctgacgatc ttccggcagg gtccggacta
    21061 gcgtcagctc atatccgcat cgaaggtgag gcgatgctcg gactggcccg gttcgaaggc
    21121 gacatggagg tgcgcttccg cgcgcagtac gcggaacgct cgatcccgtt cgtccgcgtt
    21181 tcgctgcccc ttgcggcgac cctgtacctg ctgttcctcg catgggacta cagcatcgcg
    21241 cccgaggccc tgctctacac gctgagcgtg cgcctgccgt tcagcttctt cgcggtcgcg
    21301 gtgttcggct tcaccttcct gcacacgttc gagcgctggt cgcagagcgt gctttgcgcc
    21361 accgtgatcc tgggcgcggc gggcatcgcg ctcgtgctgg cgattctgcc gcagggcttc
    21421 acctacggcg tgccgggcct gttgctcgtc atcatgtatg cctgcggcgc catccgcctg
    21481 ctgttcacct cggcgttcat cgcctgctgc acgatcgtgg cgctgaccaa cgtcctgctg
    21541 ctaaccacgg gcgccagcac gtttcaggtc ctcaacacca acgtgttcct gatcgcggcc
    21601 tcggcgatcg gcctgatcta cacggccctg ctcgaatgga cggaacgcca cgcattcgcg
    21661 ctggaggagg acctgcgcaa ggacaagcag gcgagcgaga cgttgctgcg caacttcctt
    21721 cccgatcgca tcatgagccg gttgcgcgac ggcgagcgca ccattgccga ggcggtcggc
    21781 gaggccaccg tgctgttcgc cgacatcgtc ggcttcacct cgctcacgca ccgcatggcc
    21841 cccggccacg tggtcgaggt gctgagcgtc atcttcaacc gcttcgacga gatctcggat
    21901 cagcgcggcg tggagaaggt caagaccatc ggcgacagct acatggcggt cgcgggcgtg
    21961 cgctcgccgt cgccgcgcaa cgccgaggcg atggccgagt tcgccatcga ggcgcttgcc
    22021 ttcgtgcgca gctacgccat ggagaacgac ttgccgctgc aggttcgcat cggcatgagc
    22081 acggggtccc tggtgtcggg cgtgatcggc acgcacgtgc cgatcttcga cctatggggc
    22141 gcaccggtga acgacgcgag ccggctgcag caggaaggca ttcccggcgc gatccaggtg
    22201 tcggagacga cgtactggcg gctgcgtaac aagtacgagt tccaggcgcg cggcactctg
    22261 acgctcaagc acggcgagca gatcaatgcc tacctgctga ccggccgcaa ggtggcggcg
    22321 ggcgccggcc tgcacgtgat cagcgacgcc tcgcgcgcga ccgcggacac gcttccggca
    22381 aagacgggct gagatggacg ccactcttgg gcagcagcag gtcgcccgtg ccgacgccac
    22441 agcgcgcgcc gccggctggc tgtgccccga gtaccgcgaa gtggtggacc gcactgtcga
    22501 gctgctgttg aacaccaaag cgcggctgcg cgccgcggtg ccggccggcg cggacggcgg
    22561 ccgcgctatc gagaccatca ccaacgatct gtgggccgaa tggcgcagcg cctgggtcga
    22621 tgcggccgca tccctggccg agatcgccgc aacgcccgag cacctgcggc tggccaagga
    22681 ctacaccgaa tcgcatctca cgcccgagct gatgggcgcc ccgctgtggc gccaggcgta
    22741 caccaaaccg ctcggctacc ccggcgacta catcgcgatg aactacatgt acgacggcac
    22801 gcccgagggg gatacggcgt tcgccagcgt cgcgcacgag ctggcggtgc atatcggcca
    22861 gttcgtggtg aagcgcaagg atctcgtgcg ccacgcgatc gccgaagcgg tcgccaggca
    22921 tagcggcgcc gagccgtgcg tgatcgcgag cctcggctgc gggccctcgc gggaggtgac
    22981 ggagttcctt gcgcacgacc cgcttgcatc gcgcccgacg cgtttcttcc tcgtcgatca
    23041 ggacagcgac gccctgtcct tcgccggccg gcagctggcc gcggtcgttc gcgggggcgc
    23101 cggccagggc gcggcgcgca tcgagatgcg gcatctgtcg gtgctgcggc tacttcgcga
    23161 aatcgagccc ggcgagctgc taacgcccgc agacctgatc tacagcgccg gcctgctcga
    23221 ctacttcagc gaccgtacct gccgcatcct tacgcagcgc ctttatcagg cgctgcgccc
    23281 gggcggccac ctgctgctcg gcaacatgaa ggcgggtacc gacatgctct ggcccctcga
    23341 gatgatcgcg gactggacgc tgcgctatcg caacgcggag agcatcctcg cctggaccga
    23401 gggactcgag ggtgccgaga tctcgctgcg caccgaagcc actggctacg actacctcct
    23461 gagcgtgcgc aaggcctagc gatccgatca ggcctgcgcc ggatgcggca cggcgcgcac
    23521 gccacgcagc agtactgccg cgagcccgat ccactagcgg cgcgtcgccc tagtcatcgc
    23581 cgagcgcgat cgcgaccttg ttcgggtaga aggcgagcag gtcccggata tcgagcatct
    23641 cgtcataagg ctgctcgtag ctccacaccg cgttgtccgg cccgttcacg atgcagtagt
    23701 acgacgcatc gcccttgtag gggcaatgtg tggtcagcga ggtgcgcatc agccgctcca
    23761 gcttcacgtc cttgcgcggc acgtagtaca cgggcgggta gctgccctcc cgcatctcga
    23821 gcgcttcgcg tgagtcggcg agcacctcag cgccgagcgt cacgcgcacg cgcttgcggg
    23881 ccggccgggt gacgatgtgg ttgcgctgag cattcttgaa gccaggagcg gggttgccca
    23941 tcgggatctc ctttgccggt tgcgattgcg gctttcttgt ctacccgggc gccggtccgt
    24001 gtcaagatcg ccccctcatg cccgcccgcc gccgcttcgc ccgtcgcgag gacctcggtc
    24061 tcaccaaagc cgagtttgcc gtattgcgcc gcctgaacac gccgcgcaag gcccaggcct
    24121 tcctctacgg actgccgcag aacttcgagc tgcgcggcga cacctgcaac agcgtgcgcg
    24181 acgtgctgcg cacgcggcgc gcgcactgca tcgagggggc catgctcgcc gcggccgcgc
    24241 tctgggtgca cggcgagccg cccctgctcc tcgatatgcg cgcggtgcgc gatttcgacc
    24301 atgtggtggc gctgttccgc cggcgcggcc gctggggcgc catctccaag accaacggcg
    24361 cgacgctgcg ctggcgcgac ccggtctacc gcagcctgcg cgagctcgcc atgtcgtacc
    24421 tgcacgagta ctgcaaccgg cgcgatcaca agacgctgcg cgagtattcg cgcccgttcg
    24481 atctgcgcgt gctgcgcacc gcagactggg tcacggcgcc caagggcgca tgggcggtcg
    24541 ccgagcgact cgatgccttg catcactacc ggctgctctc gcccgccgag gagcgtgcgc
    24601 tggtgcggcg cgacccgttc gagcgccgga tcggcgcgct cctgcaatac cgccgtccca
    24661 agcggctgcg gcgcggctcg ggccggtact agcagctgcg gcggtgccgt tacgcaggta
    24721 acacaccgtt acacacgtta cgtccccgcc ccaccactat gtctccgtca ccggcgcaaa
    24781 cgagttgtcc ggtgcaacgt gtaaaggaga cagacatgct gaagaagacc gctgcaatcg
    24841 ccgccctggc tggtgccacc ctgctgagca ccacccccgc ccttgccgac ggctatcgtc
    24901 acggctacgg gcatggcccg aagcgcgtcg tcgtagtgca caagcaggtg ccctactatg
    24961 gctaccgcca ggggtaccgc caggccgtcg cctatcgccc ggtttacgcg caccggcccg
    25021 tggtcgtgca tcgcccggta gtcgtccagc accggtaccg cggccacgac gtgctcggtg
    25081 gcctgatcgt tggtgccgtg ctcggcgcgg tcatcgccag ccacggcggc tactgagaac
    25141 ttcgccggcg cgacggcggt ccaacggcca gcagctgctg gccgttgccg tttgtcagga
    25201 accgcggccg tactcgacta aactgcgcaa ggaggcacga tggatcgacg cggactactg
    25261 aaagcgctgc tggcggcccc ccttgtaggg atgccctggc tcgcctatgc gcagaagaaa
    25321 ggaatggcga tggtcgaaga gctgcagcgg aactggaaga tgttcctggc cgccggcgtg
    25381 gcgacgccct ccccggagga accgctcacg ctgtccaaag acgagtggcg caagcgcctg
    25441 ccgccggctg ccttccacgt gctgcgcgag gaaggcaccg agcgcgcggg caccagcccc
    25501 ctcgacgccg aaaagcgcga gggcgtgttc gcatgcaggg gctgcgacct gccgctgttc
    25561 acctccgcga tgaagttcga cagcggcacc ggctggccga gcttcttcac cacgattccg
    25621 ggcgcgttcg agaccaagac cgactacttc ctgatctacc cgcgcaccga gtaccactgc
    25681 aagcgctgcg gcggccacca cggtcatgtc ttcaaggacg ggccgccgcc cacgggcctg
    25741 cgctattgca acaacggcgt ggcgctgaaa ttcattccca aggaaggcaa ggcctagagc
    25801 ccgagcgccc cggcgagcgg cgccgccggg tagtgaaact tgaccggccc gtcgggctcc
    25861 atgtgcagga actggtaaat gcgcgcgtcc ttcgagtcgc gcagttcggc gggcgtgcct
    25921 gcgccgatca ccacgccctc cgagatcacg tacaggtagt cggcgacctt cagtgactcg
    25981 tgtacgtcgt aagtcacgat gatggaggtc gcgccgagcg catcgttcag cgtgcggatc
    26041 agatcggcga tgacgttgag cgagatcggg tcgagtcccg cgaacggctc gtcatacatg
    26101 gtcagcatcg ggtccatggc aatggcgcgt gccagcgcca cgcgccgcgt catgccgccc
    26161 gagagatcgc ccacgcgcag cgcgtgcgcg ccgcgcagcc ccaccgcgtg cagcttcatc
    26221 agcaccaggc tgcggatcag cgcgtcgggc agtcgggtgt gctcgcggat cggaaaagcg
    26281 atgttctcga atacgctgag gtcggtgaac agcccgctgg tctggaacat catgcccatc
    26341 ttgcgccgca ggtcgtagag cggcgcgccg tccagctcgt gcaccggctg agcgtcgacg
    26401 tgcacggtgc cgcgcagcgg gcgcagctgg ccgccgatca gcttcagcag cgtcgacttg
    26461 ccgcagccgc tggtgccgag gatgccgacg accttgccgc gcggcacgga aagcgacagc
    26521 ccgcgcagca cctggttttc gccgtaggcg aagtgcaggt cgcgaatctc cacgatgttg
    26581 tcggcggcgg ccctcatgcg cggtgatagt ctagccgaac cgcatgaccg cgcccgcgcc
    26641 ctatgccgga ctgacgccgg aaaccgtgct cgacgcgctt caggtcgcgg ggctgcgcgg
    26701 cgacgggcgc ttgctcgcgc tgaatagcta cgagaaccgc gtctaccagg cttggctaga
    26761 ggacggcggc agcgtggtgg cgaagttcta ccgcccggaa cgctggagcg acgcgcagat
    26821 cctcgaagag cacgcgttcg tgcaggagct cgccgcgcgc gagattcccg cggtggcgcc
    26881 gctcgcactc gagggtcgta cgctcaacgc gcacgcgggc tttcgctttg cggtgtttgc
    26941 gcggcgcggc ggccgcacgc ccgagctcga ggacccggag accctgcgct ggctgggacg
    27001 ctacatcggc cgcatccacg ccgtgggcag cgggcgcggc ttcgacgcgc ggccggcgct
    27061 caacgtcgag accttcgggc gcgagccgcg cgactggctg ctcgcgcacg gcttcctgcc
    27121 gcccgacctg cgcgaggcgt ggcagagcgc ggcggcgctt gcgctgcagg gcgcggcgcg
    27181 ctgcttcgag cgtgccggaa gcgtgcgcat cctgcgcctg cacggcgact gtcacgccgg
    27241 caacgtgcta tggaccgacg ccggaccgca cttcgtggac ttcgacgatg cgcgcagcgg
    27301 ccccgcgatg caggatctgt ggatgctgct ctcgggcgat cgcgcatcga tggcgcggca
    27361 gctcggcctg gtgctcgaag gctaccggcc gttcttcgag ttcgagcggc gcgagctgca
    27421 cctgctggag gcgctgcgca cgcttcgcct gctgcactac tcggcgtgga tcgcgcggcg
    27481 ctgggacgat cccgcgttcc cggcggcctt tccctggttc aatacgcagc gctactggca
    27541 ggaccgcatc ctcgagctca gggaacagat cgcactcctg gacgagccac cactcgagct
    27601 cgcctagagc aggcgccagg cctcgagcag gacctgccgg gcgcggcgct gcgccgtggc
    27661 gatggcgatg gtgcggttga gccacagcgc gccggcatcg ccggtctcga agcgcatcac
    27721 ggtgcgaaag aagtattgct ccgcgggcac gtcctcgccg cgcccgagcg cggccagcac
    27781 ctcgggggga ccgtgacggt agccctggct caccacgcgg atcagggcgc cggcgtcggt
    27841 gcgaatggcg tagcgcgcgt cgatgtcgag cacgccgtcg gcgcgcacga tctgccagtc
    27901 ggcaccgccg ggcacgatct tgcctttcag ctccggaccc tcgaacgtcc caccggtgat
    27961 cgccaccacg cgccgctcgc cgagcggacc accgcccagg gtctgcagct cgccgacgtc
    28021 ggcggcaatc ttcatcagga attcgagccg cggcgccatt atcagtccgg cagatcgtcc
    28081 agcgcgcgct cgaggtgcgc gacctgcgcc cacgaccgca cgtgccacgc ctgctgcccg
    28141 atctcgatcc agttgagggc ggcattcggg atcgcgaagt cgcgcggcgc ggacagtccc
    28201 gtgccgtgcg cgtgccggtg caccatctcg agcacgccgc cgtgcgtgaa gaccagcgcc
    28261 gtttcgccgg cgtggcgcgc cgcgatgctg gcgacgcacg ccaccgcacg ctcgtagaac
    28321 ccgcgcaggc tttcgccact gccaaagtcg tagtccggat ccttggcctt gaagcgcgcg
    28381 tagtcctcgg gcaggcgctc gcgcgcctcg gcgtaggtca gggtctcgaa cttgccgtag
    28441 tggcgctcgc gcaacgccgg ctccaggcgc accggcaagc ccaggcgcgc ggccgccggc
    28501 gccgcggtct ggcgcacccg cgcgaggtcg ctcgagtaca gcgccacaaa ccgctccccg
    28561 gcgagcgcct cgcacacggc gcgcgcctgg gcctcgccca ccgccgacag gggcacgtcg
    28621 gtttgcccct gtacccgccc ctcggcgttc caggcggtct cgccgtgacg aataatgcag
    28681 atacgagaag ttcccatcta cccctgcgcc gaaggcagct atgatagcgg cgacatgacc
    28741 gattcgccgc ccgcgcacca cagcgccctg cccagcatcg agcaggagga gctgcgcgga
    28801 atctcgcgca ccgtcgccga gatcgaatgg ctgctgctgg tcctggttct cgtgtacgtg
    28861 gtgttcggcc gcccgcccgt cgaggtggag acggcgctgt cgatggcgct gttcttcttc
    28921 gccgcgttcg tcatgagctt ccgctacacc aacttctaca agcgcgagac gcgctggaag
    28981 atcgcggtgg agacgctggc gatgatcgtg ttcatcacct gggcgctgtg gttcaccggc
    29041 gggccggcga gcccgctgct caatgcctac ctgctggtcg tgatcaccgc agcgctaacg
    29101 ctgggcaaga cggtgacgct catcgaagtg gcgctgatcg gcgcctgcat cgtgctgctc
    29161 gacctgcagg tcggcggcaa ggacatgatg cagctcgcct acctgggcaa tttcgtggcg
    29221 cagctcgcgc cggtgatact ggtggcctac atcaccacca tgttctcggc ggacatccgc
    29281 tacggcctga accgcgccaa gctcctgtcg gagaccgacg aactcaccgg gctgtacaac
    29341 atgcgcggct tcatcgtgac cgcgaaccgc ctgttcgccc aggcgcagcg ctacgcgcgc
    29401 tcggcgagct tcctgatggt ggactccgac agcctgaagc aggtgaatga cagctacggc
    29461 cacgaggccg gcaaccggct cctgcagcac atcgtgaatt cgatgcaggg acacctgcgc
    29521 gccaccgacg tcccggcacg ctatggcggc gacgagttcg tcatcatgct gcccgacacg
    29581 ccggcgcgcg gcgcgctcga agtcgccgag cgcatccgca acgccgtgga ggcgacgccg
    29641 ttcgccaccg aggccgggcg cgtgccatgc accgtgagca tcggggtcgc cagctacccg
    29701 caggacgggc gcagcatcga ggcgctgctg gcgcgcgccg accgcgcgct ctatgaagcg
    29761 aaggccgccg ggcgcaatcg cgtggcgcag cacgacgtcg ccatgcagtg cggcgcgcag
    29821 cccgcgggcg gatttcagat cccgaactcg acgtaggtgt agatgaagtt cgagccgccg
    29881 tcgacactgc cgagcagccg cgacatggcg aacacgcccg agcggtggga cacgcccagg
    29941 ccgacgtagg tgttcttcag cgcacggctg ccgacgaggt cgccgacgct gaaatcgatg
    30001 gtcgggtcga ggtaggtcaa cagcttgtgg gtgttctcgc cgcggcgggc ctggtcgcgc
    30061 gcctcgatgg ttgccacgcg ttcggcgtac gacagccccg agccgatgcc gatgcgggtc
    30121 ttcacgcgct cgcgccacgg aaagccgtac cagtaagact tgaagtaggc attgagctgc
    30181 cagtggtcgg gctggaagcc gttctcgtcg tggtgcagca ccccgatata gcccgcgatg
    30241 tccatgttcc agccgtacag gcgctcgatc agcgtgcggc cgagctcgac cgaggtcacg
    30301 cgcgtttctt ccggagtgcg ggtagaggtg cagctgagct tgacgatctg cagcacgtcg
    30361 cattcgctcg ccttgccgtg atagaccttc acccacagca ggcgcgacgc gctccaggcc
    30421 gcgggctcgg gtgaatgctc gtagagcagg ccgacggtgg ccgcggcttg cacccggttc
    30481 tcgacgatcg ggctcgcgcg cacgctgctc gaccactgcg tcgccgagat gccgccgaga
    30541 agttgccagt tccgactcag cgggtagctc gcgtagagcg cgagctcgag attgagcccc
    30601 gagtccgcga cgtaggccgg ccgcgccgcg cttgcctcgc ttgcgcgcac gccgtagtag
    30661 tagtcgttca gtttcgtgtt gcgccatgcc agcatcgcct gcggccacag gcgcagctcg
    30721 ccgcgccgga tttcctccgc caaccgcagg cgcacctcgg tgccgcgcga cgcgtcgtcc
    30781 acgttgtgca gcagctcgac accgagcgcg ccccacgcca tgcgccggcg gtagccgagg
    30841 ccgaggtctg tgccctgctc gcgcagcgcc atgccggcca gggatgccgg cgcgcgcacg
    30901 agcggcgtgc cctcgtagcg atgcgcgaga aacacgtcga cgcgctggtc ggcgtcgaga
    30961 tcgagcttca ggcccacgcg atcggaatgc aggtatgcgt gctcgccggc gtaaacgttc
    31021 agcggcaggt agtcgtaacg cacgccgtcg ccgagatacg gcgagcgctc cgcgcgaacc
    31081 gcactgcccg agccggcgcc accgacgtcc agcgcggcgt cgaatctgtc gccgtaggcc
    31141 gacgccacgc cgagccacat tgttaacagg atgaatccgg ccgcacgcac cgcactttcc
    31201 tccccatggg aacgcgcgag cttacatgga aatcacgcta cgataacgct gccacttgag
    31261 cacgcttagt ccaggagtcc gcatgccgct tcgacatctc atgcccggta cgctcgttgt
    31321 ttgcgcgtgc ctcgccgcag cgcccgcaac cgcgatggag ccgtggaacc tgctcgcgca
    31381 atcgcgcaag cagacgccgc agccgccgtg gtccaccggc gacgagcgcg gcatggccaa
    31441 ccagatcggt cctgcgacct gggcgcgctg cgcctggcac ctcgggcagc gctcggcaaa
    31501 ggcctacgag ctgtcccacg agcgctcgag caccatgccg ctgtcgccgt ttaccgggcc
    31561 gtacgtgcaa acgcccaagc tcacggcggg cctgcccggc accggccacg gcttcaacgg
    31621 cgagtcgctg aacgagggcg ccgagcccgc cgcgcagggc acgcagatgg acgccctcgg
    31681 ccacttcgcc ttcatgaccc agccctggga cggcaaaccg cctttccccg cggacactct
    31741 gcgctattac ggcggcttca cgcaggccga cgtgaagcct acgccgcaat cgccgcttgc
    31801 gcggcttggc atggaaaagg cgccgccgat cgtgacgagc gccgtgctgc tcgacgccaa
    31861 gacgcatgtc ggcggcggtg cggcaatgaa agccggcgag gtggttacgc gcaaggacat
    31921 cgaggcgatg ctccgtgccc agggcctcgg caagcgcggc atccagtacg gcgacgtggt
    31981 gtacgtgcgc actggttggg gcgagcgctg gcgcgacccg gacaccgaga aaagctacta
    32041 cgcgaaggcg ccaggcctct cctacgacgc ggcgcagtac ctcggcgagc gccgcgtcgt
    32101 cgccgtcgga ctcgatacgc cgttcatcga cccggtgccc gagggcatgc tgacgggcaa
    32161 ggcgagcccc gcgccgggca cccccgaagg cctgcccttt gccgtgcacc accatctgct
    32221 cacgcagatg gggatacacc acatcgagaa cgcgaagctc ggcgagctgg cgaacgacaa
    32281 ggtgtggacc tcgtgcacca tgatcctgcc gttgcgcgag cgcggcgcgg cgggctcgcc
    32341 ggtgcggccg gtcgccatcg gcgtgccggg ccggtaggcc ggcctcaggc gcgcgcgtcc
    32401 ggatccagcg gataggcggg ccggcgcacg cgcttgaacc gcagctgcgc gtagtcggag
    32461 gtgcacacgc cctcgcccgc gcactcgacc acggcatgcg cgaggggtcc caggccggcg
    32521 cgccagtgca cgcggctctt tagcatgacg tagcgcttct tcgccggatc gagcccgagg
    32581 gcgcgaaacg cgcccaggtc gtacggctcg gtgtggcgcg agatcaccgc gacctgcacg
    32641 cgcccggtgt cgagcactgc ggacgggccc atgtccatcc gctcgccctt cgccatcggg
    32701 ccttcgttcc ggtaagtccc gtcgatgagc cgcgtcacgg tgccgctcac ttcgagcggc
    32761 cggcccttca ggccgagcgc cggcatgtcg agcttgccgc ccagaggcag cgtgacgcgc
    32821 gcgcccttcc cggccgcctt catcttctgc accgcgtccg gatcgaacac ggcgaacgcg
    32881 catacgtccg ccaggcgcgc ctcgagaatg ccgccgagca ccgtcatcgt gtccatggtg
    32941 ccgcccgagg cgcagttgtc gtagtgatcg agcagcacga tcggaccgcc ctgcagcgag
    33001 cgcgcgcgct cgagcgaagc ggcgagcggc tcgacgcggt agacgaactg ctcgcggctg
    33061 tgccacgcca tgtcgagcag ctcgtcggcg aggcgctgcg cccgcgcgcg atcgccttcg
    33121 gtaaccacca ccgccgacac gcccgcgtag ggaatgtccg cgtgcggaaa gccgacgaag
    33181 aaagtggcgc tcagcgcttc gcccttcgcc tccatctgcc gcgcgcgcgc ctggatgctg
    33241 cggttgggct cgtcgaggct cgactggcgc atgacgtgcg gcagcatcgg gcgccagccc
    33301 caggccatcg ccggccgcgg cctgcccgcg agcatgccca ggaccggcct tgccgcgcgc
    33361 aggccggttt cgtacatgtc gatgtgcgga taggtctggt agccggcaat caccgtggcg
    33421 tgctcgccca tcgccggaaa gagattggtg tgcatgtcca gcgcgacgcc gatcggcacc
    33481 tgcggcgcga cgcggcggat gcgcgcgaga agctcgccct cgccgtcgtc gtggcgctcg
    33541 gtgaccatcg cgccgtgcag gtcgagaagc accgcgtcgc agcccttggc caccgcgtcg
    33601 acgatgcggc ccgccatgtg ctcgtaggcc gcgcgctgca ccgggccgct cggcgcggcg
    33661 ttgcccgcca ccggcagcac cgcctcggcg tccgccttct cggcgagatc gaggaatgcg
    33721 ccgagcgccg tgccggtgcc cttgaacgcg gcgcggatcg cctcgccttc gaggggtgcg
    33781 gcgcgcccgc ccgcaaagcg cgcgaggtcg gtcgctaccg gcgagaacgt attggtttcg
    33841 tgcttcatct gggcgatgac gaggcgcatg gcggtgctcg ggttcgatta gactgcggga
    33901 ccatgcgagc ctacttcatc cgcgccggca acggccaggc gagggtcgaa tcgaaagacg
    33961 ccgcgctccc ggagccgggc ccgcagcaga tgcgggtccg cgtgcgggcg gcgggcctca
    34021 accgaggcga gttcatgggt gccggcgcca ccgccaagcc cgccggcacc gatgcggcgg
    34081 gcgaggtcga aaagctcggc cctggcgtaa cgcggttcaa ggccgcagac cgcgtcatgg
    34141 gccgctgccc gggagccttc gccgaatacg cgctcatgga cgagcgcgag acgatggcgg
    34201 tgcccgaaaa gctctcctgg gaggatgcgg cggcagtgcc gatcgtgttc ctcgtcgtgt
    34261 acgacatgct ggtcgcgcag ggccgcctga aggcggacga atggctgctc gtcaccggcg
    34321 tcacctcggg cgtcggtgtc gccgcattgc agacggcgaa ggcgctcggc gccaaggtca
    34381 tcggcacctc cggctccaag gacaagctcg cgaaacttgc gccgctcggg ctggacctcg
    34441 cgctcgccac gcgcaagcct gacttcgccg atgcggttct cgacgctacc ggcggcaaag
    34501 gcgcgaacct gtgcgtgaac aacgtcggcg gcacggtatt cgccgagtgc gtgcgtgcgc
    34561 tcgccttcga gggacggctc gcggtggtgg gctacatgga cggcgtgctg aaggccgaga
    34621 tcgacctcga cgccctgcac gccaagcgcc tcacgctctt cggtgtctcc aacaagctgc
    34681 gcaacgccga gcagcgcgcc acgacggtgc gcggcttcag cgacaaggtg ctgccgctca
    34741 tcgccgaggg ccgcatccgg ccgctcatcg accgcgtcta tccgttcgac gagctgcccg
    34801 ccgcggccga gcgcctgcag gccaacgccc acgtcggcaa gatcgtcgtg atacttaaat
    34861 agggacagac ctcattattt cgctatgcga aaaaatgagg tctgtcccta attagatggc
    34921 ctttgcggcg cgcaggctcg cgatttcgtc ggcgccgtag ccgagctcag ccaggatctc
    34981 gtcggtgtgc tcgccggctt ccggcgctgc gcgcaacatg cgcgccgggg tgcgcgagag
    35041 cttcaccgcc tggttgacga cgcggaactt gccgagcgcg cggctttcga cttccacagg
    35101 cacgccgagg tggttcacct gcgggtcggc gaacacctgg tccatggtgt agatcgggcc
    35161 gcagggcacg cctgccgcgt tcagcgcctc gatccattcg gcgctctttt tcttccccag
    35221 cgccgcgttg atctcggcat tcagtgcggc gcggttcttc gcgcgcgccg gctggccctt
    35281 gaactcgggc cgcgcgagca ggtcctcgcg gccgagcgtc gcgcacagcc gcgtccacat
    35341 gccctcgccg gaggcggcga cgttgatatg gccgtcggcg gtcttatagg ccgaggtcgg
    35401 catgctggtc gggtgatcgt tgccgacctg cggcgccacc tcgccctcct tcaggtaacg
    35461 cgccgcctgg aagtcggtca gcgtgatccc cgcctgcagg agcgaactct gcacccactg
    35521 ccccttgccc gagcgctcgc gctcgagcag cgccgtcatg atgccgagcg cggcgaacag
    35581 ccccgccgac acgtcggcca ccgccgcgcc cacgcgcacc ggtccctgcc ccggcaggcc
    35641 ggtgatcgac atcaggccgc tcatgccctg cgcgatctgg tcgaagcccg gacggtcgcg
    35701 gtacggcccg tcctggccga agcccgagat gctggcgagg atgatgcgcg ggttgatcgc
    35761 cgcgagcgac tcgtagtcga tgccaagccg gaacttcacg tcgggacggt agttctcgac
    35821 caccacgtcc gcggtcttca ccaaccgccc gaagacctcc ttcgcctgcg gctgcttgag
    35881 attcagcgtc agcgagcgct tgttgcgctg caagttctgg aagtccgacc ccgtgcgcgc
    35941 gccgcccatg ccttcgttcg ggtcgagacc cggcggcgac tcgatcctga tcacgtcggc
    36001 gccgaagtcg gcgaactggc gcacgcaggt gggcccggcg cgcacgcgcg acaggtcgag
    36061 aaggcgcagc tgcgagagcg cgccagaacc gccaagcttg gtcatggcgc gaatcttatc
    36121 ccgagcgcgg ctaggcctgc gcgtcgcgct gctgccaggc ggcgcgccgg tagcccgagc
    36181 gccgggcgac cgggcgcgcc gcgtactccc ggatccgcgc caggacctcg cgccgcacct
    36241 cggcaggcag cccgccgccg gcgcgctcga agcgctgcag cagcacctgc aggtcgggcg
    36301 cggcaccggg cgcgccgtcg tggaacaggc gctcgccgcc gagccacacc agcgcccgct
    36361 cgctggcgcc ggcgccgtag cacagctcga agcgcgccag cgccccgagc gcgtcctgcc
    36421 agacgaacag atcgaagtac tcgtcgtgga accagcgccg gcgcagcggc gcttcatcgc
    36481 ccaagcccag gatctcgcgc attgcccggt tcgactctcg cgcgcggcgc cagttcccgc
    36541 cgcctagcgc accgcggtga cgcgcgtatt ggcgagcacc aggcggtcga ccaggttgcg
    36601 gaccagcgcg cgcgtcagct gcagctgcgc cccgccgctc atccgtgcca gcgccgcgtg
    36661 atcgaactcg gcgagcagca gcttggtcat cgactccacc gtggcatgcc gcggcagctc
    36721 gccgccccag atgtaggcca tctcgccgaa gcattcgctg tggctgacga cgttcagcag
    36781 ccgcccggcc ttggtgacct tgacctcgcc ttcggcaaga aagaagaagc tcttgccctt
    36841 gtcgttctcg cgcacgatgg tccggccctt ttcgacgcgc gtccacttgc ccgcgctcgc
    36901 cagctcccac aggtcggcgt catccaggcg cgagagcatc tccacgcgct tcagcgcaac
    36961 gtacttctct gtatcgggga tggcgctcgg cggcacgctc tggcgcacca ccttggcaag
    37021 ctcggccgcg aactcggccc aggtcggata gcgcgcttcg ggcttcttgc gcagcgcgcg
    37081 caggatcacc gcgtcgagct cccgcggcag gtccctgcgc acctcgctcg gcggcagggg
    37141 ctcgagctcg aggatcttct tcagcagcgc gtccaggttt tcggcgcgca gcggccgctg
    37201 cccggtgagc atctcgtaca gcaccacccc gagcgcgtac atatcgctgt ggtgcgtaag
    37261 ctcgtgcccg gcgatctgct cgggcgacag gtagtacggc gagcccattt ccgcgctctg
    37321 caccgcctgg gtcttcctca ggaaggcggc gccgaaatcg gcgatcttga cgttcgtgcc
    37381 gcctgcgagc atcaggtttg cgggcttgat gtcgcgatgc acgatcccct cgcgaaaggc
    37441 atagtcgagc gccccgcagc acttgaagcc gatctgcagc acgtcggcca ccggcaggag
    37501 cttgcccggc tgcgtgtgcg cggccagatc gcccccggtg accagctcca tcgcgatgta
    37561 gccggagtcc tcgcccacca cggcatcgag gatcgaaacg atgtgcgggt gcttgagctt
    37621 gccggcgagc gaagcctcgt tctggaactg cgagcggtac acggtgccga actctgggtc
    37681 gcggaacacc tcgggctcga tggtcttcag cgccacgtcg cccccggaaa acgtgtcgcg
    37741 cgccaggtac acggtgccgg tcgcgccctt gccgagcaac ttgtgcacgt cgtacttgcc
    37801 gacctgcagg ctcagtccga ggtcgatcgt gtccggcacc gccgatatcc cttccgtttg
    37861 gtctcccggt ccgggatggt actgcatgcc gcttgccgag cgcgcgcgca ggcgacaaaa
    37921 tcggcttctc aatttgtccg gtggcggcgc gcacatggaa cacaaggaag ttccggtcga
    37981 gtatctcgag ttcggcatgt tcgtcgccaa gctcgaccgg ccgtggaccg aaacgccgtt
    38041 cgtctaccag ggcttcgtgc tgaagaccga gcagcagctc gaggcgttga agaagtactg
    38101 caaagtggtc tatgtcgacc ccgagaggcg ccaggaggtc gtggcggcca ggctgctctc
    38161 gctgcctccg ggctcgacgc gccaggtgat cgccgagcaa tcgaaagaga aagtgcgcgg
    38221 caccgtgatc tacaaggagc tcgccacggt cgaggacgag ctgccgcgcg cgcgctcggt
    38281 gttcacgcag accaacgcca tcgtgcacac gatgtcgcgc gcggtggtca ccggcaacgc
    38341 gctcgacggc aaccgcgcca ggcaggcggt gaccgagatc accgattcgg tggtgcgtaa
    38401 ccccgacgcc atgatgctgc tggtcaccat gcgcgacaag gccgaggaga cactgaaccg
    38461 ctcgacccag gtctcggtct atatgaccgt gttcggccgc ttcctgcagc tcgagcgcga
    38521 gcgcctggaa attctcggcc tgatcggcct gctgcaggac gtgggcatgc tcaagctccc
    38581 cgccggcatt cccgggcggc gcagcgatct ggcgcaggag gaggacgcag tcttcaagtc
    38641 gcacgtcacc cacagcgtcg agatcctgtc gcagaccgcg ggcctgccgc ccgagctgcc
    38701 gagcctggcg tcgctgcacc acgagcgcta cgacggcagc ggctacccgc gcggcctgaa
    38761 gggcggtgcg atttcgcaga ctggcgccat cgccggcatc gtcgacacct tcgacatgct
    38821 cactgcgccc gcgccctacg gcatgcagat gccgccgtcc aacgccctga acgtgctgtt
    38881 ccagaaccgc ggcacgcagt tccacgccgc gctcgtcgag cagttcatac agtgcgtcgg
    38941 cgccttcccg gtgggcagcg tggtcgagct gaacagcggc gaggtcggcg tggtgatcac
    39001 gcagaacctg gtgaagcggc tgcagccgcg cgtgatggtg gtgcaggacg ccaacggcaa
    39061 cccgatccat ccgcacaaag tgctcgacct ggtgaacgag ccgatggcca cggccgacga
    39121 gccctaccgc atccggcgca cactcgacca gtcgacggtc aagatcgact cgcgcgagct
    39181 gtttctgtga attcggcgct ggaccgcgcc gccatcctgc gcctgctgga gcagttcggc
    39241 ctcggcgatg acgcgctcgc cgcgctcgag gcgctgggtg cggcgctcag caggcaatcg
    39301 cccgaggcgc tgcaggcggc gcgcggcgag agcgcgctcg ccggcgcgac gtcaccgttc
    39361 ttcctgccgc tgctgcccgc cgccatgacg gagtcgcagc gcgcgctggt gggcgcgatc
    39421 gcgcgcggcg ccctgggcgg cgaggcgctg ccgcagctgc tgctcgactg ggccgccacg
    39481 ctcaaccccg aggagccgag cgagcgcgcg ttgcgcgact gccggccgct gcaggaactg
    39541 ctgcgctcgg gggcgctgca cgtgtacgcg ggcgacgccg cgtcctgcgc cgcggcgctc
    39601 gcggcggccg agcgcctggg cgccgtgcag ctcgcgctca tcgccgagac cgcggcgcgc
    39661 ggcgcgcccg gcggcgcgcc cgccgccttc ggcctgtcgg acttcatggc gcaggtggac
    39721 gcctgcgtcg ccgcgcgcat cgccgacggg cgccagatgg cgctgctgtt cgtcgactgc
    39781 ggcgtcatcg ggcgcatcga cggcgtatgg ggctacgtcg tcggtgacgg cgtgcgcgag
    39841 cgtctgggcg cgcggctgcg caccgaagtg ctgcgcaccc aggacctgct cgggcacccc
    39901 gggcgcgacc agttcatctg cgtgctcgcc gacctgcaca gcgccggggt ggcgacgctc
    39961 gccgcggaga agctgctgcg cgcgctcgac cagccgctgt gggtggacgt ggacgagctg
    40021 tacccgcgcc cggcggtcgg catcgccgtg tacccggagc acggcacaag cggggtggag
    40081 ctgctgcagc gcgcgcgcgt ttccgcggac gcagcgcgcg agcgccccga gcgctacgcg
    40141 ctctacagcg ccgaccagga acgttccgag atcgagctgc tgctctacga gaaccggctg
    40201 cgcgcagcga tcgcgcagga tgcgctccac atcgtgttcc aggcgcagcg cgacgcgcgc
    40261 accggcctca tcgtcggcgc cgagagccag ctgcgctggc gcgacgcggg cctgggcgcc
    40321 gtgccgatgg agaaggcgat cgccgccgcg gagtccgcgg gcctggtgaa cgaggtgacg
    40381 ctgtggctgg tgaacggggc gctgcgcaac tgtcgccagt tgcgcgacac cgcgggcctc
    40441 gacctgcgcg tcggcgtcac gctctcggcg aagagcctgc gccagcccga cctgcccgac
    40501 ttcgtcctga gcgcgctcaa gacctggaac ctgcgcccga gccggctgtc gctcgagatc
    40561 accgacacct cggtgctggc ggcgagcctg gagtcggtgg agaccctgca acgcctgcgc
    40621 gaggcgggcg tgcgcctgtc gctcgacgat cccaacgccg gctacgcctc gctcgcgtgg
    40681 ctcgcgacgc tgccgttcca cgagctgcgc gtcgatctcg ccaacatcgg caacatcgtc
    40741 gattcacagg agcacctggc gctggtgcgg gcactggtgg agctcgcgca ccggctgaag
    40801 atggacgtgc tggtcgcggg cgtggacgac gaagcgagcg ccgcacgcct ggaaaccctg
    40861 ggctgcgacc tgatgcaggg cacgttcatc agcccgccgc tggagacgac cgcgttcgtg
    40921 gaccggttcc gcggggactg aggggtaagg cgccgatggg tgacttcggc atcggccagc
    40981 cagtggagcg cttcgaggac ccgcgcctgc tgcgcggcgc ggggcgcttc gtccacgacc
    41041 tgagcctgcc cggccaggca tgcctcgtgc tggtgcgttc gccgcacgcg cacgcgcgca
    41101 tcgtcgccct cgacgccgcc gcggcgcgcg ccgcgccggg cgtgctcggc gtgtacagcg
    41161 tcgaagacct gcgcgccgac ggcaccggca ccagcgcggt ggcccttgcg cgccggcgcg
    41221 ctgtgcgcgc tgcgcgtcga gagccacccg gtgccgacgg cgctcaaccc gctcggggcg
    41281 aagggcgcag gcgaagcggg caccgtcggc gcgctcaggg cgcgcagaag tagttgcgct
    41341 tgccaagctc gacgccgttg tgccgcggga tggcgcagcc cgcggtggga ttgtgtcagg
    41401 gcagtggcaa agcgatgatc ttgatgcccg ggcgcgaacc gcggcgtccg gacggcagtc
    41461 gaatcatcat ttccgcaagg gagaacgacg ccatgcgcaa cgcaagtctc gcagtcctgc
    41521 tcgccactgc cctcgccgcc tgcagcgagg ccgagccgcc caagtcggcc gcggcgcccg
    41581 agccgcccaa gccggtcgtg ctggcgccgg ccccggcgcc aacgccgtcg cccgcgcccg
    41641 ccaccgcgcc ggtggtctcg cccgaagagc agctcgccga gcgcgtgaag caggcgctgc
    41701 gcgacacgcg caaggtcgag ggccagggcg tcggcgtgcg cgtcgccggc ggcacggtca
    41761 ccctctacgg caccgcgccg agcgccgacg agcggcgccg gatcgaacag ttcgtcgccg
    41821 gcgtcgaagg cgtccaggcg gtggtcagca agctggtgat cgtgcgcggc tcgtagccgc
    41881 ggcgcgaaag cccccgcggt aggcggcgcg ccgctcttcg ccgtcccgcc ccagcgcgca
    41941 gtacagcgca tgcggggtga cgagcgcgtc ggcctcgccg agcgcattgg cgcggtagct
    42001 cgaccagcgg tagtgcgccg gatggcgcac caggcccgcg cgcaccgggt tgagctcgat
    42061 atagcgcatg caggcaagga catagcgccg cgcatagaca ggcgtggcgt cggcggcgag
    42121 tgctcgcatg gcgtggacca gcggcatcgc gccgtccgcc ccgaggggcg tcagcagcaa
    42181 gtgtgcgtgc gtgcccatca ggacgtaggc atgcaccgcg cagcccgcgg ccgcgcgcag
    42241 acgctcgagg cacgcagcgg ggtcggccgc gctgcgcctc agcacgacgt gcagcgggac
    42301 gccgggcagc tgcggacagg gcatgcgggt catgagcaag caacgacccg ccgcgcccgc
    42361 ggttgacagc tcagcgcagc agttccgcgg ccttcctcag gaacgccgcg ccgcgcgggc
    42421 ccactacttt ctccagcatc gggcgatgcc tctccacgaa cgcgcgcgcc tcgtcgggcg
    42481 acttgcacgc atcgagctgc atgacgatcg gatcaccctc cggcccaagc gcctcggtca
    42541 gcgcctgcgc cagctgaatg cggatctcct tgaaatcggc ggcgggcgcc gccgcctcga
    42601 caaagccgtc ggcgagcagc cgctcgagca tcggccggat atcgccgagc tgctcaaact
    42661 gcttcaccaa gtccgcgccg gtcgccttgc cgttcaccac gatgagcagc gtgcgcagct
    42721 tctgctccag ccggtgcttg cgcgtctcga tctcctccac gcccttgccc gatttcttca
    42781 ggaccgcttg caggtccatg cctggcctcc cagacagctt gcctgcggca aagcgtaccg
    42841 caggcacgtg cgacagggaa ccgggccgcg gtaggatgcg tcctccgcat agcgaggccc
    42901 cacatgagca ctcccgaaaa agccgccgcg caggcgccgc acgacccgat ccaggagctg
    42961 gcggagaaga tctacgtgca gctgtgcggc cgtatctacg gccagggcgg tgagaagccg
    43021 cagcccaagg ccgtcgcgca gttgtgcttc aagctcgcgg agaccttcat cgccgcgaac
    43081 tacgagttca accccacggc gatcgccgcg cgcgaggcga agaacaaggc ctcggtcaag
    43141 ctcgactcgg tgcagatcga tttcggcgca gtggacaagg gctaggtccg ttgccatgct
    43201 tcaggcaccg gtgcgtaagc gcacttcggg ggttctctaa agtggggtca tggcttcggc
    43261 ggtgccggag cagacgatta accacttgaa aggagcctca gcatgaagca ctaagttctc
    43321 gcagtcgacc tcggcaccgt gtttgcgctg ccggccctcg ccaacaacga aattgattcc
    43381 gcctacaccg gcgcggcgca gaaatccacg ccgatcgccc cggtgctcgc caacgccgaa
    43441 ctcggcacca aaggcaacca gctgtaagcc ctacggcaag tcctctccgc agttccggca
    43501 gcccgcagtc cctcccgccg cggtgctgct gtttgacgta aggtcgtgcg ccaccagggg
    43561 ttttcggaaa cctgcgccgg ccggaaactt gggccgccgt ccgcgggtca caaggtcatg
    43621 gccaagcttg cccgcccgct tctggaaaca ctcgatgcgc gcgagcgcgc gttgcgcgac
    43681 aagatcgccg agcgtcgcga agcgctcgag gacgcctcgc gtccggctga tccggccggg
    43741 gacgaggccg acaaggcgtt cgagcgcacg tgcgccgagg tcgagcacga cctgatcgag
    43801 atgtcgctgc gggaactcac cgagatcgcg gcgctgcgcg agcgcctggc caacggcacc
    43861 tacggcgagt gcctcgagtg cggcgagccg atcccgccag cgcggctcga ggtgaacccg
    43921 acggcccgcc gctgcgccga ttgccaggcg cggcacgaga agctctccgg ggcgctctag
    43981 gcgagccgcg ccaagtcgtt tttcaggttc ggcggcggtt gaaacaaccc cgggccgcgc
    44041 ttcgcgccga gctcggcgag gttgacgaaa tgggctagcg gcgtgtccgc cggcgcgcct
    44101 gcgcggcggc aagcgcggcg ccgacgatgc cggcgtcgtt gcgcagctgc gccggcacca
    44161 ccggcgtcgg cagcgtcagg cgcggcagga acttgcgcca gcgccggctc acgccgccgc
    44221 cgacgatgat caggtcgggc cagaaatagc gctgcagcag gtgcaggtag gcgtccacgc
    44281 gccgtgccca ttttttccag ctgaggctct cgtcttcgcg cacggccgcc gacgcccagg
    44341 tctcggcgtc gcggcccttg agcgtcaggt ggccgagctc ggtattgggc acgagacgcc
    44401 cgtcgatgaa cagcgcggtg ccgataccgg tgccgagcgt caccatgagg acgaggccgc
    44461 cgcgcccgcg gcccgcgccg aaggccatct cggccaggcc cgccgcgtcg gcgtcgttga
    44521 tcacgcccac cgctcgtccc acggcggcgc cgagcagggc gtgcgcatcg acgcccagcc
    44581 agctgcgcgc gatgttggcc gccgtgcgca cgcgtccgtg ctttaccacc gcgggcagcg
    44641 cgcagcccag cggccccttc caggcgaagc gcgccgcgag ctgcgcgacc gcgcgcgcca
    44701 ccgcctccgg cgtcgccggc tgcggcgtcg cgatgcggcg gcggcgcgcc agcagccggc
    44761 cgctgcgcgt atccaccggc gcgcccttga tgccgctgcc gcccacgtcg atgccgagaa
    44821 ccttgatggc cgtgcccctc ccgttcaagc cgcggcgatg ccgagaaagg atgccgcgac
    44881 gccgaccagg ctctcgcccg cgacaagccc ggccgccgcg gcgatggtaa agcgcgcggc
    44941 gagcgacgca aagcgccgct ccaggagcca cgcggcgagc gcgcccagcg ccatggtgaa
    45001 gcagatcgag gcggggatga cgaaggccag gccgaacgcc accgcgctcg gcgcgtaacg
    45061 ccagcgcggc tcgagcgcgc cgagggcgac gcccgcgagc gcccccagcg ccatggcgat
    45121 gcgagcgctc gcgggcaccg ccgtgagtcc cgcagacatc gcctccgcga cggccttcca
    45181 ggtcgccacc gccggcgccg gccactcggc ggtgagcagc atgcccgcgg gatccgggat
    45241 cagcgcgaga tagaccaccg tccctacgag cgcgcccgcg agcacgccgc acacctgcgc
    45301 caccgactgg cgcaccggcg aggcgccgat cgcgtggcct accttgtagt cgttcatcag
    45361 atcggcgcac tgccctgcgg cgccgccggc gacgttcgcg gccatcaggt tcgttaccgc
    45421 ctggccgggc gcggcgaggc cgatgccaag ctgcgacacc ttgccgatgg cgccgatcgg
    45481 cggaattccg gtctcgccaa ccacgcgcgc cgcgaccgcc gcgagcacga gcgccagcgg
    45541 caccgcgagc agcgcgatga caaacccgat gtcgaacagc atcacctgca tcgctaccgc
    45601 gagcgcgcac gcgagcgcca ggccggcgac ggccaccggc gtccagtgcg ccccgatcgt
    45661 gaggcgcgcg ccgcgcacct gcagcgcgaa gcgggtggcg gccgccgtca ccatcagcgc
    45721 gacgccgggc cacagcagcc attccaccag cggcccgaag ccgccctgcg ccacccggcc
    45781 cgtcccgagc agccacggcg cgagtgcgcc ccaagcgacg agcgctcccg cggcgagcga
    45841 gaggcccgcg cgcagcccga tgatggcgcc gaagccggcg agcagcggcg acggatcgag
    45901 cccgatcgtc aggcgcgccg ccgccgcgct cggcgcccac gccgcgagcg gcgcgaatgc
    45961 cttccaggcg gcggcggcga gcgccgcgcc ggcgagcacg gaaatgcgcg cggtggcttc
    46021 ccgcccgtgc gcgaagacgt cgcgccgcgt ctcgagcgtg gcgacgcccg ccgggaaccg
    46081 cagcggatgc tgcacgatga gcgcgggcct gagcgcccag gccacccaaa cgccgagaaa
    46141 gctcaccgta aacacccagg cgacgagcgg ccaggggtcg aacccgcgcc cggtgatcag
    46201 cgtataggcg ggaatcggcg cgaccagtcc gccggagacg atggacgcgg ccgcgctcgc
    46261 cgcggtctgg ctgatgttgc tttccttcat cgtccactgc ggtgcccgcg cggcgcgctc
    46321 cgcggcgcgc cagaaggcga gcccggcgag cagcgccgcg atcgacatgt tgaacgacca
    46381 gccgatcttc aggcccgagt agacgttgca cggcgcgagc agcgcgccga gaaggacgcc
    46441 cgtcagcagc gcacgcggcg agagttcgcg ctcggccgcc atccagagcg cagcctagcg
    46501 cttctcttcg gccgccgccg ccggccccga cggggtcagc agcgtgctct tcgcctcctt
    46561 catcggctgc ttggtgaagc ccttgagcga gctgatgtcc tcggcatcgc gcgggatctc
    46621 caccgggctc gtcgcgtagg cggtcaccag ctcggccagg gaccgcagcg catgcatgtg
    46681 gatgcgctcg tggccgtgcg aggcgtcgat gccgaaggcg atgagcgcgg cgcgcacgtc
    46741 gtgtccggcc tcgacggccg aggcgctgtc gctgcggtag tagcggaaca cgtcctgctg
    46801 gtagccgatg tcgttctcgc ggcacagctt gatgagcttg cgcgtcaggt ggtagtcgaa
    46861 cggcccggtc tggtcggcca tgccgatggt gacgccgaac tcgtcgctcg cctgcccggg
    46921 cgcggcagtg ccgttgtcga tcgacacgag cgaggccacg tcgtgcgtca gcaccgagga
    46981 cgcgcccacc cccacctcct cggagatcga gaagatgaac agcatgtcga cgggcagctt
    47041 gccgccgtct tcctgcagcg ccttcagcgc cgcgagcatc gatgcgcagc ccgccttgtc
    47101 gtcgagatgg cgcgagacga tgaagccgtt ggccaggaac tcggcgcgcg tgtcgatggc
    47161 gatcgtgtcg ccgatggcaa tccccaggcg ctccaggtcg cgccgcgtgc gcgccagcgc
    47221 gtcgacgcgc agctccacgt gcggccagcc cacggggtgc gtgtccacct cctcgttgaa
    47281 ggtgtggccc gaagccttca gcgacaggat gctgccgcgg taggacccgc gctcggtgaa
    47341 cagggtgcag cgcgcgcctt cggcgaagcg cgccgaccag tgcccgatcg gcaccacctc
    47401 caggcggccg ttgtccttga ggttcttcac ctgcgcgccc agcgtgtcga ggtgcgcgat
    47461 catggcgcgc gcccccttct gttcggcgcc cggcaggcgc gcgcggatcg cgccgcggcg
    47521 cgtgagctcg tagttcacgc cgagcttgcc gagctcgtca caggtcatgc gcacgatggt
    47581 gtcggtgtag cccgagggac tcggggtctc cagcaggcgc ttgagcacat cggcaaggta
    47641 ggtgacgtcg atgttcaatc gagacatgcg tttctccttc agcgcgcggc gcgcttcacc
    47701 gcggtcggca tggagtgcgg gaacaagaga tcgacgaagc gctccgcggt cggctgcggc
    47761 tcgtggttgg cgaggccagg tcgctcgttc gcctcgatga accagtagtc gggctggcgc
    47821 gggttgcgca ccatgaagtc gatgccggtg accggaacat ccagagcgcg cgccgcgcgg
    47881 atcgccgcgt cgatgagcga cggatgcact tcggcggtga cgtcgtggat cgtgccgccg
    47941 gtatgcaggt tggcggcctt gcgcacgttg atctcctggc cgctcggcgg cacgtcgtcg
    48001 agcgtgtagc ccgccttggc gacgcagcgc tcggtctcgg cgtccagcgg gatcttcgac
    48061 tcgccgccgg tggccgccgc gcggcgccgg ctctggcgct cgatcagctc gcggatcgtg
    48121 ctcgcgccgt cgccgatgac gcgcgccgga cggcggatgg cggcggccac cagcttgtag
    48181 ccgatcacca ccaggcgcag gtcctcgccc gtcacgcatt gctcgatgag cacgcggtcg
    48241 ctgtgcgcct gcgcgcgctc gaccgcggcc atcgcctcct ccagcgtctc cagccccacc
    48301 gccacgccgc ggccctgctc gccgcgcgcc ggcttgacca ccacgctgcc gtgcttggca
    48361 aggaacgccg ccaggctctc gcgcgtatcg aactcgatct cgtcgggcac gcgcacgccc
    48421 gcgcgcgcca ccgcgcggcg cgtcgccgcc ttgtcgtcgc acaggctcat cgccacggcg
    48481 ctcgtcagct cggacaggct ctcgcggcag gcgatcgagc gcccgccgta ggtgaggcgg
    48541 aaaaagccgc cctcggcgtc cagcacctcg acgccgatgc cgcgccggcg cgcctcatcg
    48601 acgatgatgc gcgcgtaggg gttgagctgc gcttccggcg gcgcgccgac gaacaagcgc
    48661 tcattgatgg cgttcttgcg cttcacggtg aacaccggca accgcgtgaa gccgagcttc
    48721 tcatagagct cgatcgcctg gcggttcgat gccatcaccg acaggtccat gtggcgcacg
    48781 ccgcgcgcct ggaaatactc ggccaggcgc cgcaccagcg tctcgcccac cagcgggcgc
    48841 gcggcctgcg gatcaacggc caggcaccac agcgaggcgc cgcgctcggg atcgccgaac
    48901 gcgcgcaggt ggtcgacgcc ggtcaccgtg ccgatcacct gctgcgtcac ctcgtcctcg
    48961 gccaccaggt acacgatggt gcggctgtcg cgatggctga acatgaactc cgcgggcacc
    49021 gtcaccatgc ggtgcgcgag gtagatgcgg ttcaccgcgt cggcgtcggc ctggctcgtt
    49081 acccagcgca cgacaaagcc gcgcgggcgc ttgcgcgagc cgcggtaggt gctgaggtca
    49141 agccggtagg tgttcgacgg atccaggaac agcgcctgcg gcgccgccgc cagcaccagg
    49201 tgcgggtcgc gcacccagga ggcgatgtcg cgccgctcgg gcccctcctg cgcgagggct
    49261 gcggcgagcg acctggcatc ggggtaggtc tgcgcgaaca gcagtcgtcc ccagccgcag
    49321 tcgagcgcca cttcactgtc gggcacgtgg gccacgtact tgcgctgcac ccggtgcgtg
    49381 tacgcggcgt gcgggcgccg cttgggccgc gctcggttca ctttggcttc catgtcatac
    49441 cccgtgcgcc tgcagccaca gctcgagcag cgccgcctgc cagagctccg agccctgcag
    49501 cggcgtgatg tgcgccttgg gatcggcgaa cagctgctcc aggtagtcgc ggcggaacag
    49561 gccccgctcc ttcgccgcgc gcgcgccgag cgcctcgcgt gcgagatcca ggaacggacc
    49621 ctccaggtac ttgagcgccg gcaccgggaa gtagcccttc ggccggtcga tcaccgccgg
    49681 cgggatcacc tgccgcgcca cgtccttcag gatgcccttg ccgccgtccc ggagcttgtg
    49741 gtgcggcggg atgcgggcgg cgagctcgac gagctcgtgg tcgaggaacg gcacgcgcgc
    49801 ttccagcccg gcggccatgg tcatgttgtc cacgcgcttc accgggtcgt ccaccagcat
    49861 caccgtggtg tcgaggtgca gcgcacggtc caccgggtcg ccggcgtcgg acgccgccat
    49921 gcgctcctgc accagctcgc gcgcgtggtc gagcccgacg tactccgccg acacctggct
    49981 cgaataggcg gcgaagtcgc ggtccatgaa ggcgcgcgcg tagtcctgcg ccggatcgcc
    50041 ggagcccacg agcttcgggt accagtggta gccgccgaac acctcgtcgg caccctggcc
    50101 cgactgcacc accttgacgt ggcgcgccac ttcctgcgaa agcagataga acgcgacgtt
    50161 gtcgtaggac accatcggct cggacatggc aagcaccgtc gccggcagcg catcgagcag
    50221 tcgcgaggac tggatgaaga ttctctggtg ctcggtctcg aagcgctgcg ccacgatgtc
    50281 ggaccactgg aactcgtcgc cggactcgcc gtgcgcgccc tcgaagccga tggagaaggt
    50341 gcgcagatcg acgtgcccgg cctcggccag cagccccgtg atcaggctcg aatcgacacc
    50401 gcccgagagc agcacgccca ccggcacgtc ggcgatcctg cgccgctcga ccgcgaggcg
    50461 cagcgcggca agcagctccg cgccccagtc cgcggcgctt ttctggatgt cggcgctcga
    50521 gcgctcgaac gaaagcgacc agtacacgcg ctcgtgcgcc ttgccgtcgg gctcgatcga
    50581 gcgcacggta gcgggcggga gcttgcgcac gcccttgagg atggtgcgcg gcggcggcac
    50641 cacggcgtgg aacgacatgt aatggttgag cgccaccggg tcgatcgagg tgtccacgtc
    50701 cccggcggcg agcagcgccg gcagcgtcga ggcgaagcgc acccggccct tggtttccgc
    50761 gtagtacagg ggcttgatgc ccaggcggtc gcgcgcgagc gtcacgcggc cgctgtcgcg
    50821 ctcggcgacg gcgaacgcga acatgccctg caggcgctcg ggcgcgcgct cacgccaggc
    50881 gtgaaacgcc ttcagcacca cctcggtgtc gccgtcggaa aagaagacat agcccagccc
    50941 ctgcagctcc ttgcgcagct ccttgtagtt atagatgcag ccgttgaaca cgaccgacag
    51001 gccgagcacc gggtcggtca tcggctgctc ggcgcgctcg gagaggtcga tgatcttcag
    51061 gcggcgctgg ccgaggccga tgcgcccgcg caccaagagg cccgcgccgt cggggccgcg
    51121 cggcgccagg ctgtcggtca tcgcctcgag ggaggcggcg gaggccaggc gaccgtcgaa
    51181 tgtcagctcg cccgcaattc cacacatggc tgtcccttgt gcatgatttt aagtggctgt
    51241 attatatatt tatattactt tgaggtcaaa ccattaccga cgctgaagca cgagttctct
    51301 cggccatccg ccgcatagtg cacgcggtcg accggcactc caagacggtg ggaaaggcct
    51361 cgggcctgac gctgccgcaa ctggtagtgc tcgccgcgat ccgtgcccgg ggcgaggtga
    51421 cgagcgcgcg cctgtccgcc tcggtcagcc tcacgcctgc caccgtcacc gtcatcctcg
    51481 acaacctgga ggcgcgcggc ctcatcgagc gctaccgaaa cgcggcggat cgccgcgtgg
    51541 tgcactcgcg cctcacgcca gcgggtgccg acgtgctcgc gcgcgcgccc ggcttcctgc
    51601 acgagcgctt cgtgcggcgc ttcgcgcagc tgcccgccgt ccggcaggag gtggtgctcg
    51661 cggcgctgga ggaagtcgcc gccatgatgg gcgcgccgga agccgcgccg gcccccgtgc
    51721 ccctcgaggc cgggctcgcg gccgagctcc cctagccgat cgccttggca aagcgattct
    51781 tctacggctg gcgcatggtc ggcgccggcg cgggcatcca gttcctgcag gcgagcttgc
    51841 tgctgcagtc tttcggcgcc tacgtcgccg tgctgcagga cgagcgcggc tggtcgaaga
    51901 ccgagctgtc cttcgccgcg gcgctgcacc agatggaggc cgcgatcctc ggcccgctgc
    51961 tgggctggat catcgaccgc ttcgggccgc aaggcgtgat ccgcaccggc gtggtggtgc
    52021 tcggcgcagg cttcatgctc ctttcgcaga tcgattcgct cgccgcgttc tacggcgcgt
    52081 tcatcgtggt ggcgctcggc tcgagcatgt gcggcttcat gccgctcaac atctcgctca
    52141 tctactggtt cgagcggcag cgggcgcgcg ccctgtccac gctcggcttc ggcctggcgc
    52201 tgggcggtgt cgccgtgccg ctggtggcct ggtgcatggc ggcctgggga tggcgcgtca
    52261 ccgccttcgc ctcgggcgtg ctgatcctcg ccgtcgggct gccgctcgcc ttcgtcatgc
    52321 gcggccgccc ggcgcgctac ggcgaaaccg tcgacggcga gccgccgctc gccacgccga
    52381 gccagctgcc cgcgctggac acggcgaagg ccgcgcgcga cttcaccgcg cgcgaggcgc
    52441 tgcgcaccag cgcgttctgg ctgctctcgc tcgggcacgg cttcgcgctg ttcgtggtca
    52501 cggcggtgaa cgtgcacgcc atcacccacc tcaaggaggg cctgggctac ccggtgcaga
    52561 ccgcggcgct gttcatcatg gtgatgacct tcgcgcagat cggcggcatc gcgctcggcg
    52621 cggccttcgg cgaccgcttc gacaagcgcc tggtctgcgc gggctgcatg atcctgcaca
    52681 tgacggggct gctgtttctc acctacgcga acggcgcggc gatggtgctc gcgttcgccg
    52741 ttctgcacgg caccgcctgg ggcttgcgcg gaccgttcat gcaggcgatc cgcgctgaat
    52801 acttcggcgt aagcgcgatc ggcatgatcc tcggcctgtc cttcatgatc atcgtcgtcg
    52861 gccaggtcgg cgggccgctg atcgcgggcg gcctggcaga cctgaccggc aactatcgcg
    52921 ccggctttac cgtgctcgcg ctgctcgccg gcgtaggctc gctcttcttc ctgctgtcga
    52981 agaagccggc gcggccgcgc ggctaggccg ccttcaggcg ccgcaccggc gcgcgcgcga
    53041 actcgcccgc ctggtagtcg gcgatcgcct gctcgatctc ctcgcgcgag ttcatgacga
    53101 aggggccgta ctgcaccacc ggctcgccga tcggccgggc ggcgaggacc agcacgcgcg
    53161 cgcccgtgtc gccggcgacc atcgacagcc tgtcgcccgt cgacagcagc ccggcggcgc
    53221 gatgcggcag gggctttctg tcgtcgccca cgaaggcctc gccctcgtag gcgtagacaa
    53281 aggcggcgtg ctccgccggc agcgcgcctt cgaacgtcgc accaggctcg agatgcacgt
    53341 cccagtagtg cggttcggtc gtgccgccgt gcaccgcgcc gcgcgtgccg ccgaactcgc
    53401 cggcgatcac cttcacggcg ccccgctcga tcgccaccga ggggatctcc ctcgccgcga
    53461 tgtcgcggta ggccggcggc tgcatcttct ccttcgccgg caggttgagc cagagctgaa
    53521 agccgcgcat gcgcccttcg ctctgctgcg gcatctcgga gtggatcagg ccgcgcgccg
    53581 cggtcatcca ctgcacgtcg ccgggcccga ggtcgcccgt gttgccgccc gagtccttgt
    53641 gctgcatgtg gccgtcgagc atgtaggtca cggtctcgaa gccgcggtgg gggtgcgagg
    53701 gaaagcccgc cagatagtcg ttcgggtcgt ccgaatagaa ctcgtcgagc atcaggaacg
    53761 ggtcgtggcg cgccagcggg ctcgcgccga gcgagcggcg cagcttcacg cccgccccgt
    53821 ccgaggccgg gatcgagggg atcactttct gcagggtgcg tatggtcatc cgcccgatat
    53881 taggccgacg gaccggcgtt acaagggccg gaccaggatc acggcgtgcg cgacgcgcgc
    53941 gctgcgtaca ctattccgca tgcccgcgac gcgcaagatc atccacctcg acatggacgc
    54001 gttttatgcc tccgtcgaac aacgcgacga cccgacgctg aagggcaagc cggtcgcggt
    54061 cggcggctcg ccgcaggggc gcggcgtggt cgcggcctgc agctacgaag cgaggaaatt
    54121 cggcatccat tcggccatgc cgatggcgcg tgccgtgcgc gcctgccccg ggctggtgat
    54181 cgtgcggccg gatttctcca agtacaaggc ggtgtcgcag caggtgatgg cgatcctgcg
    54241 ctcggtgacg ccgctggtgg aaccgctgtc gctcgatgag gcttatctcg acgtcaccga
    54301 gaacgcgctc ggccagccgc tcgccatgga ggtggcgagg catctgaagg cacgcatctt
    54361 cctggagact gcgctgaccg cgtcggccgg tgtcgcgccg aacaagttcc tggcgaagat
    54421 cgcctctggc tggaacaagc cgaacggcct gaccgtcatc gcgccggagc gggtcgaggc
    54481 cttcctgcag cagctgccgg tggaagcgct ctggggcgtg ggcccggtga cagcgaagaa
    54541 gctgcgtgcc ctgggcatcg ggcggctggt cgacgtacgc agcgcggatg aagcgcggct
    54601 gcgccgggcg gtcggcagcc acgccgattg gctgcagcgc ctgtcgcgcg gcgacgatcc
    54661 gcggccggtg acgccgaacc ggccgtggaa gtcgatctcc tgcgaggaca cctaccccga
    54721 agacctcagc gagctgcgcg agatgcgcgc ggagatcgag cgcctggcca agcgcacggc
    54781 gtcctcgctg gaaaagaagg gcctcgcggc gcgcaccgtg accatcaagg tgcgctacgc
    54841 agatttcacg acggtgacgc gcagccacac cgaggcgccg gcgacgaacg acgcggcgcg
    54901 cctcgtcgag cgcgcacgca tcctgctcga gcgcaccgac gcgggcaagc ggccggtgcg
    54961 cctgctcggc gtcggcgtgc atggtctcgc cgcggccgga ggcgcgtcca cccccgggcc
    55021 gctgccgctc gaggccaagg ggccgctttc ccccgcggat ttgctctaga tcaaaggagc
    55081 gggcggatcc cggcattacc gtgccgactg gagtaaacca aggcatggat cccagcaagc
    55141 tgtcccagtt tgaaatcgtt gcgcggcagg atttttccga cgtcacctac ctgctcgagg
    55201 tgcgccatcc gctgatggcg aaagcggccc ggccgggcca gttcgtgatc gtgatggcgc
    55261 atgaacatgg cgagcgcatc ccgctcacca tcgcggattt cgaccgggcg aaaggcacca
    55321 tcaccctggt gatccaggcg gtgggcaaga ccacgcgcga gatgcagcag tcctgcgtgg
    55381 cgggtacgac gctgcacgcg ctcgtcggcc cgatgggcat cccgagcccc gtgggcagcg
    55441 cgaagaaggt ggtctgcgtg ggcggcggcc tgggcgtggc gccgatcttc ccgcaggcgc
    55501 gcggcttgaa ggaagcgggc gcctacgtga tcggcgtgct cggcttccgc accaagagcc
    55561 tcgtgttctg ggagcacaag ttccgcgcct gctgcgacga gctgatcctg tgcaccgacg
    55621 acggctcggc cggcctcaag ggcctggtca ccgagggtat ccggcgcgcc atcgataagc
    55681 acggcgacat cgacgaggtc atcgccatcg gcccgccggt gatgatgaag ggctgcgccg
    55741 aagcgacgcg cgcgcacaag atcaagaccc tggtgagcct caatccgatc atggtcgacg
    55801 gcaccggcat gtgcggcggc tgccgcgtga agatcggcga ccaggtcaag ttcgcctgcg
    55861 tcgacggccc ggacttcgac ggccacaagg tggatttcga cgacctgatg acgcggctgc
    55921 ggcgctacac gcaggtggag cgcgccgcgg ccgagcgctg gtcggcgaac tgccgcatga
    55981 aggacatggc ggacccggcc gcgcccgccg tcgagctgct cgagctgccc gatcccttcg
    56041 acgacgtgaa gggcgggtag cgtgccggcg gcgaagaaga agaccatccg caccatcccg
    56101 caggaacgca cgccgatgcg cgagcaggag gcgcgcgcgc gcgcgaagaa tttcgacgag
    56161 gtggcgtgcg gctactcggt cgacgaggcc ctgcgcgagg ccgagcgctg cctgctgtgc
    56221 ccggacgagc cctgcgtcaa aggctgcccg gtcagcatcg acattccggg ctttatccgc
    56281 aagatcggcg aaaagaacta ccgcggcgcc tatgacgtca tcgcccagac caacctgctc
    56341 gccgcggtct gcggccgcgt ctgcccgcag gaaacccagt gcgaggaggt gtgcaccgtg
    56401 ggcgaatcgc tcgagccggt cgccatcggg cgcctggagc gcttcgtcgg cgacgccgcg
    56461 atccgcgagg gctgggtcaa cgtgccaccc atcgagccca acggctttcg catcggcatc
    56521 gtcggctcgg ggccggcagg catggcgtgc gcggcggaca tggccaaggc cggctgcgag
    56581 gtcagggtgt tcgaggcctt ccatcagccc ggcggcgtgc tgaagtacgg catcccggat
    56641 ttccgcctgc ccaacgaggt ggtcgacgcc gagatcgaga acctgcgcaa gatcggcgtg
    56701 cgcttcgaat gcaacacgct cgtcgggcgc ctgttcacca tcgagcagat ggtcgacgag
    56761 ctcgggtttc acgccgtgtt catcggcacg ggcgcgggct atccgtcgat gctcggcgtc
    56821 ccgggcgact cgctgaacgg cgtgctctcg gcgaacgagc tgctgacgcg ctgcaacctg
    56881 atgcgcgcgc gcgatgccga cttcgacacg ccgatcccgc tcggcaagcg cgtcgccgtg
    56941 atcggcgcgg gcaataccgc gatggacgcg atgcgcgtgt gcctgcgcct gggcgcggag
    57001 aaggtctact gcgtctaccg gcgctcgcgc gcggaatgcc cggcgcgcgc cgaggaagtg
    57061 caccacgccg agcaggaagg catcgagttc cactggctca ccgcaccggt ggcgatcctc
    57121 gacgacggca agggcggcgt gcgcggcatg cgctgcatcc gcatggagct cggggcccca
    57181 gacgattccg ggcggcgccg cccggtgccg gtggcgggca gcgagttcga cttcgaggcc
    57241 gaccaggtgg tgtacgcgat cggcaccaac gccaaccaga tcatcggcca gacctcgcgc
    57301 ctgaagctcg acaagcgcgg ctacatcgcg accgatgaag acctcgccac ctcgatgccc
    57361 ggtgtgtacg ccggcggcga catcgtcacc ggcgccgcga ccgtgatcga ggcgatgggc
    57421 gcgggccgca aggccgcgcg caacatgaag gcctatctcg gcatccgcga tccggcgcgc
    57481 gccgcggccg gggagacgct cttcggcatc gaccgcgccg agcgcaattt cgtgcgcctg
    57541 cgcgccgccg cctgagagac ccatgccaac cgcccagaaa gccgtcacca ccctcaaaga
    57601 gcacatcgtc gagatcatca gcgactcggg cgagggcgcg cagcgctgcg gccagtcgct
    57661 cggcgccatc gccgcgcgca tgggcaacgg catctggacc gtggagatca tcccggccga
    57721 gatccgcccg ccggcgcgct ccatcgccgg ggcgagcggc aaccgcattc gcatcggggc
    57781 gcagcgcgtc accaacggcg gcgacgagac cgacctggtg gtcgccttca acgagcaggt
    57841 gctgctcggg cgcgtgcgcg cgggcgagct caagcccggc tgcatcatcc tgctggaaaa
    57901 catgtggcgc agccacggcg acgcggcgat cgccgcggcg tacgtggaga cgcacgaccg
    57961 cctcgtcgag gccggctatc gcgtgatcga aattccgatg gagcaggagt gccgcacgct
    58021 ggtctccgat ccgcgccgcg gcaagaacat gttcgccctc ggcatgctgt gccatctcta
    58081 cagcctcgac ctgcagctcg cgcgcgagca ggtcgccctc gccttcggca agaaggaggg
    58141 ctcggtggtg gcgcagaacg tgaagctgct cgaggcaggc cacgcgtggg cggaggccaa
    58201 cctcgagttc aggtaccgca ttccggccgc gcccgtcacg acgccgcagg tcgtgatcaa
    58261 cggcaatacc gcgatcgcgc tgggcgtgct cgcctcgggc atggaaatct gcgccatgta
    58321 cccgatcacg cccgccacgt cggcctcgca ctacctgtcc gacgtgttcg agaaggtcgg
    58381 cggcgtggtg caccaggccg aggacgagat cgcggcctgc gccttcgcca tcggcgcgtc
    58441 gtatgccggc aagtgcacgg tgaccatcac ctcggggccc ggctattcgc tcaagcagga
    58501 ggcgatcggc cttgccgtga tgggcgagat cccgctggtg gtggtgaacg tgcagcgcgg
    58561 gggccccagt accgggcagc ccaccaaggt cgagcaggga gacctgctca ccgcgatcta
    58621 cggcagccac ggcgacgcgc ccaaggtggt gatggcgccg agcaccatcg aggactgctt
    58681 ctactcgatc atcaccgcac ggcgcatcgc cgagaccttc aacatggtgg tggtggtgct
    58741 ctcggacgcg agcctcgcga cctcgcagca gccgttcgcg cgccccgagt tcagcgaatc
    58801 ctgggtcgcg ccgcccgtcg accagagcgc gatccccgag ggcatgaagc cctacgactg
    58861 gaacgaggcc accggcctcg cgcggcgctt catccccggc caaccgaacg gcatgcacac
    58921 gctcaccggc ctggcgcacg accgcgccag ccacgtcgcc tacgaccccg agatcaacga
    58981 ggagagcatc cacttcagga gcctcaagct cgccgcgctg cagaagacgc tgaagacgcc
    59041 gcccgtgttc ggcggcgact cgggcgagct gctcgtggtc ggctggggca gcacccaggg
    59101 cgcgatcgag gaagcggtcg ccgcgctgcg cgaggaaggg cgcaaggtgt cctcgctgca
    59161 ccttcgcttc ctgcagccgc tgccgggcgg cctgaagcaa atcatgcagc gcttccgccg
    59221 ggtgatgacg atcgagggca actggagcga ccggcccgac gacgccatca tcgacgagga
    59281 caaccggcgc tactcggcgc tcgcactgct gctgcgcgcg cgcaccctgg tcgacgtcga
    59341 ctgctggagc gaagtcaaag gccagcccat caagccgagc accatccggc gcgtgctgct
    59401 cgacgcgctc aagcccaagg ttaaggagcg cgcgcgatga ccatgatggc caccgaatgc
    59461 ctgctgcagc tgtgcgagca gcgctacgag atcgaggatt tccagagcgc cgtgccgcgc
    59521 tggtgcaccg gctgcggcga caacgcgatc ctcgccgcgg tgcagcggct gtgccgcgac
    59581 gagggcctgc ggccggaaaa gaccgtgttc gtatcgggca tcggctgctc gagccgcttc
    59641 ccgcactaca tgcgcaccta cggctttcac ggcatccacg gccgcgcgct gccggtggcc
    59701 gagggcgtgc gcctggcgcg gcccgacctc accgtgttcg tgaacaccgg cgacggcgac
    59761 tgctgctcga tcggcgcggc gcactggatc cacgccatcc gctacaacat gaacatgacg
    59821 gtgttcctgc acgacaacca gatctacggc ctgaccaaga tgcaggcctc gccgacctcg
    59881 ccgcgcggca cgaagagcaa caccaccccg cgcggctcga cgctcgaggc gctgcagccg
    59941 ctctccgtca cgctcggggt gcagaacgtg tccttcgtcg cgcaggcggt ggactggatt
    60001 cccgagtcgc tctacgaagt cgtcaaggcc gcctaccacc accagggctt ttccttcgtg
    60061 cgcatcgtgc agcgctgccc ggagtggctg ccgaaagtct gggacccgtg gctgcacgac
    60121 ccgaagcgca tcctgatgct ggaccacccg cagggcctcc gggtgagcga cgggctgaag
    60181 caggtctacc ggagcaccga tgcgcacgac ccgtcgaata tcgaccgcgc gcgcgagatc
    60241 gcgtcggaca ccgaccgcat tccggtgggc atcctgtacc gcaacccgga ggtgccgtgc
    60301 tacgaggagc tgcgcgcgac gcccggactg cgcaccatcg cgaacctgaa gcaggggctc
    60361 gaggccgagc tcgacaagtt caccttctgg ccccagtgag gcgctgtccg tgcacgccga
    60421 agtccatccc gacgccgcgt tctacgtgac cggccaccag gcggctgcgg caccgggcac
    60481 gcccgcggcg ccggcgctgc gcccggcgct gttcgccgcg taccgagatt tgaccaggtt
    60541 gcgttacgac ttcccgctgg tgctgcgcgc cgatgccgcg gacgggcggg tctacgagcc
    60601 gctcaccgcg cttgtcgagc gcctgatcga gggcggcgcg gaacgggcga aggcgctgcg
    60661 catcgaggac gagctgcgcg cggccgctgc
//
LOCUS       DBNHKE010000165         6814 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1139836, whole genome shotgun sequence.
ACCESSION   DBNHKE010000165 DBNHKE010000000
VERSION     DBNHKE010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1139836"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="AC16O9_11375"
     CDS             complement(<1..1527)
                     /locus_tag="AC16O9_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6517255.1"
                     /translation="MNDPRASEGSLEQPPASYAGHTRFTQLEYEAVLANAWIGIAFSR
                     DRKFFLCNPKFAELFGWEADELIGQPGEVVYPSTESYEALARIAVPVLSAGKQLDLDW
                     EMRRKDGSTFLARMIAKAISTTDVRRGTVWIVDDVTTARRHGDEVARLLREQEVIFAT
                     ASVGIVFLRDRRIVRCNARYEQMYGYAPGELNGRSARELYLSDEDFERVEKSYAQMRG
                     GVHAEDTLPRRRKDGSVMWVRAVGRAIDPREPHKGAVWTNEDFTDRRRAEGEMQRLLA
                     EQEALLNNVVVGIAITHERKVVRCNRRFEELFGYEASEANDMSARQLYFTDAEFELAA
                     ANYDELNRGGTHTREQWLRRRDGTGFWCRLSGRAVRPGDAARGAVWLFEDISEAKRRE
                     EEVRRALAEQNLILDNATIGITFLRERIFQRCNPRMEEMLGYAPGELIGKSTRVIHVS
                     REVYEELGSVAYAVLARGEAYTDERELRRKDGSQLWCKVVGRAIDPAQPQEGSIWIYD
                     D"
     gene            complement(1524..2354)
                     /locus_tag="AC16O9_11380"
     CDS             complement(1524..2354)
                     /locus_tag="AC16O9_11380"
                     /inference="COORDINATES: protein motif:HMM:NF039714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HIR6517256.1"
                     /translation="MSARLAAVLLLALAAGCSALGFAYNNADTWLRWQAGRYLDLDSG
                     QTGELEARISAFLGWHRAEALPQYARLAREAVARLERGASREDLVWGYDAVREQSRAG
                     LRRVSAELGDFLDRLTPAQIAHLEARFAEDNRKYAERWLDGTPAQRRERRYRRLVEVL
                     QGWVGELNDAQRERLRQYSESAPLNAEGRDRDRRRLQAELLAMLRARASAGRLADWAA
                     NWERGRDPGFAAENRAGTERLLALLAELERSLSPAQRRHAVQRLAGYARDFQVLADAR
                     "
     gene            complement(2351..3094)
                     /locus_tag="AC16O9_11385"
     CDS             complement(2351..3094)
                     /locus_tag="AC16O9_11385"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIR6517257.1"
                     /translation="MSKWLEGRVVGQTRWTGRLCSLQVRAPFGPFAAGQFTKLALDIG
                     GERIARPYSLVNAPDTEPLEFYYNVVPAGPLSPRLAALEAGDAVHVAPNPAGFLVLRE
                     VPEAEHLWLVSTGTGLGPFLSILRTDEPWRRFSRVTLVHATRTAADQAYRETIGGIAR
                     ARGAQFGFVAFVSREPLAGALSGRVPEAIRDGRLERAAAAELSAARSQVMLCGNPAMV
                     SDVTAVLEERGMRKHRRRAPGHITVETYW"
     gene            complement(3132..4118)
                     /locus_tag="AC16O9_11390"
     CDS             complement(3132..4118)
                     /locus_tag="AC16O9_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517258.1"
                     /translation="MGNALRWIGLALLATAPLAGMGNYPLHLLIMTLLWAFVYTGWAV
                     MGRLGLVSLGHGAFLGIGAYVVVMGWNHFGLSPWFGIPLALGVAGVVAILIGYPCFRF
                     KIVGHYFALVTLALSEVARLIIVALRDQTGGSLGVTPVTALADASWSLSALQFSSKLV
                     WFYIIFGFWLAGLWIWTRVDRSMARLALQAISQEEEAAASVGMNVTRLKLTVTVISAL
                     MTCMGGALYAQYQLYVNPETVSGIGISLQMVFGAIAGGMFVRLGPTVGAAFTLLLAEV
                     LRLALGHDIHGLDTTLYGLMLVLFIIFMPKGILGAAQEAWEKRRGAKAAAVA"
     gene            complement(4120..4986)
                     /locus_tag="AC16O9_11395"
     CDS             complement(4120..4986)
                     /locus_tag="AC16O9_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517259.1"
                     /translation="MEFFDVFLLEAVINGLLLGGVLALLALGLNLIFGVIDVVWICYA
                     ELVMIGMYAVYYSHNVFGWPLWLAMLVAILAVAVLGALLHQFVIRPVLAAEPINQLLV
                     TGGVLFFLQAAATLAFGVEFRNIGVNLPALQFGDMYLSFARLIAFLVALGGMIGVYLF
                     LTRTFVGTAIRAISQDRAIMPLMGVNANRIFVITSALGGALAGLAACLLVLQYDIHPA
                     IGLTFGPITFMICVLGGLGNMLGGFIAAFIFAQFISVGGYILHIEWGYVFAFLFFIAM
                     MFWRPQGLLGGK"
     gene            complement(5000..5704)
                     /locus_tag="AC16O9_11400"
     CDS             complement(5000..5704)
                     /locus_tag="AC16O9_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517260.1"
                     /translation="MLNVSNVSAGYGGFQALFDVSLEVKAGESVAVIGPNGAGKTTLL
                     RVISKLIDPRSGEISMEGRPLAAIPAHEVIASGIAHVPENRRLFAKLSVEDNLRMGAF
                     MPAARAKFAERLEFVYSLFPRMRERRGQVAGTLSGGEQQMCAIGRALMSGPKLLLLDE
                     PSAGLAPVVVQSIFDLVRRICKEGYTVLIVEQNIRQVLKVVNRAYLLETGRIKLAGTS
                     AQMLESDLIKKAYLGL"
     gene            complement(5704..6462)
                     /locus_tag="AC16O9_11405"
     CDS             complement(5704..6462)
                     /locus_tag="AC16O9_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517261.1"
                     /translation="MLTVTDLTKQFGGFTAVNRVAFELEEGEILGLIGPNGSGKSTTF
                     NLIAGALAPTAGSIRFRDREIAGQSAARVAHYGIARTFQIPRPFRRLSILENVALAAF
                     YGQAERPSREQAFAQAGEALGLVGLSADPAARVDGLGAAGLKKLELARALATRPQLLL
                     ADESLGGLDHSEMDQAADMLAGIRDKRGITIIWVEHIMGVLMRVVDRCIVLDHGEVIA
                     AGRPDEVAHDRKVIEVYLGSDAGDVQATVAARLG"
     gene            complement(6530..>6814)
                     /locus_tag="AC16O9_11410"
     CDS             complement(6530..>6814)
                     /locus_tag="AC16O9_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517262.1"
                     /translation="FFTDVLPRAIKKYGGVDPEALRKAALETDIPVGGTIQGYGVKFY
                     APGSEMAGQNERSSPVVMQYIGGSTKIVWPTAIKTADPLLPIPKSSAYRR"
BASE COUNT         1092 a         2430 c         2316 g          976 t
ORIGIN      
        1 gtcgtcgtag atccagatcg agccctcctg cggttgcgcc gggtcgatgg cgcggccgac
       61 gaccttgcac cagagctggc tgccgtcctt gcggcgcagc tcgcgctcgt ccgtataggc
      121 ctcgccccgc gccaacaccg cataggcgac gctcccgagc tcctcgtaga cctcccggga
      181 aacatggatg acccgggtgg acttgccgat cagctcgccc ggggcgtagc cgagcatctc
      241 ctccatgcgc ggattgcagc gctggaagat gcgctcgcgc aggaacgtga tgccgatggt
      301 cgcgttgtcg aggatcaggt tctgctcggc gagcgcgcgc cgcacttcct cctcgcggcg
      361 cttcgcctcg ctgatgtcct cgaacagcca cacggcgccg cgcgcggcgt cgcccggccg
      421 caccgcgcgg cccgagagcc ggcaccagaa tccggtgccg tcgcgccggc gcagccactg
      481 ctcgcgcgtg tgcgtgccgc cgcggttcag ctcgtcgtag ttcgccgccg ccagctcgaa
      541 ttcggcatcg gtgaaataca gctgccgcgc cgacatgtcg tttgcttcgc tcgcctcgta
      601 gccgaacagc tcctcgaagc gccggttgca gcgcacgacc ttgcgctcgt gcgtgatggc
      661 gatgccgacc accacgttgt tgagcagcgc ctcctgctcg gcgagcaggc gctgcatctc
      721 gccctcggcg cgccgccggt cggtgaagtc ctcgttggtc cacaccgcgc ccttgtgcgg
      781 ctcgcgcggg tcgatggcgc ggcccacggc gcgcacccac atcacgctgc cgtccttgcg
      841 ccggcgcggc agcgtgtcct cggcgtgcac gccgccgcgc atctgcgcat aggacttctc
      901 cacgcgctcg aaatcctcgt ccgacaggta gagctcgcgc gccgaacggc cgttcagctc
      961 gcccggggcg tagccgtaca tctgctcgta gcgcgcgttg cagcgcacga tgcgacggtc
     1021 gcgcaggaac acgatgccga cggacgcggt ggcgaagatc acctcctgct cccggagcag
     1081 ccgcgccacc tcgtcgccgt ggcgccgcgc cgtggtgacg tcgtcgacga tccacaccgt
     1141 gccgcggcgc acatccgtgg tgctgatggc cttggcgatc atgcgcgcga ggaacgtgga
     1201 gccgtccttg cgccgcatct cccagtcgag gtcgagctgc ttgcccgccg agagcaccgg
     1261 cacggcgatg cgagcgagcg cctcgtagct ctcggtcgag gggtacacca cctcccccgg
     1321 ctggccgatc agctcgtcgg cttcccagcc gaacagctcg gcgaacttcg ggttgcagag
     1381 gaagaacttg cggtcgcgcg agaacgcgat gccgatccag gcgttggcga gcaccgcttc
     1441 gtactcaagc tgggtgaagc gggtgtgccc ggcgtagctc gccggcggct gctcgaggct
     1501 tccttcgctc gcgcgcgggt cgttcatcgc gcgtcggcca gcacctggaa gtcgcgcgcg
     1561 tagcccgcca ggcgctgcac ggcgtgccgg cgctgtgcgg ggctcaaaga acgctccagc
     1621 tcggcgagca gcgcgaggag gcgctcggtc ccggcgcggt tctccgcggc aaacccggga
     1681 tcgcgcccgc gctcccagtt cgccgcccag tccgccaggc gcccggcgct cgcgcgcgcg
     1741 cgcagcatgg caaggagctc ggcctgcagg cggcggcggt cgcggtcgcg cccctcggca
     1801 ttgagcggcg cgctttcgct gtactgccgc agacgctcgc gttgcgcatc gttcagctct
     1861 cccacccaac cctgcagcac ttccaccagg cgccggtagc ggcgctcgcg gcgctgggcc
     1921 ggcgtgccgt cgagccagcg ctcggcgtac ttgcggttgt cttccgcaaa gcgcgcttcg
     1981 agatgcgcga tctgcgccgg cgtcagccgg tcgagaaagt cgccgagctc ggcgctgacg
     2041 cgccgcaagc ctgcgcgcga ctgctcgcgt accgcatcgt aaccccaaac cagatcctcc
     2101 cgcgacgccc cgcgctcgag ccgcgccacc gcctctcgcg caaggcgcgc gtactgcggc
     2161 agcgcctcgg cgcggtgcca gccgagaaag gcgctgatgc gggcttcgag ctcgccggtc
     2221 tgcccggaat ccaggtcgag atagcgcccc gcctgccagc gcagccaggt gtcggcgttg
     2281 ttgtaggcga agccgagcgc gctgcagccc gcggcgagcg cgaggagcag gaccgccgca
     2341 agccgcgcgc tcaccagtag gtctccaccg tgatgtggcc cggcgctcgg cggcggtgct
     2401 tgcgcatgcc gcgctcctcg agcactgcgg tgacgtcgct caccatggcc gggttgccgc
     2461 agagcatgac ctgcgagcgc gcggcgctca actcggcggc cgccgcgcgc tcgagccggc
     2521 cgtcgcgaat cgcctcgggc acgcggcccg agagcgcccc agccagcggc tcgcggctga
     2581 cgaaggctac gaagccgaac tgcgcaccgc gtgcacgcgc aatgccgccg atcgtctcgc
     2641 ggtaggcctg gtcggcggcc gtccgcgtcg cgtgcaccag cgtcacccgg gaaaagcgcc
     2701 gccagggctc gtccgtgcgc aggatcgaca ggaacggccc gagtcccgtg ccggtggaga
     2761 cgagccagag atgctccgcc tccggcacct cgcgcagcac gagaaagccc gccgggttcg
     2821 gcgccacgtg caccgcgtcg cccgcctcga gcgccgccag gcgcggcgac agcggcccgg
     2881 ccggcaccac gttgtaatag aactcgagcg gctcggtatc cggggcgttg acgagggaat
     2941 aggggcgcgc gatgcgctcg ccgccgatgt cgagcgcgag cttggtgaac tggccggcgg
     3001 cgaacgggcc gaacggcgcc cggacctgca ggctgcacag ccggccggtc cagcgcgtct
     3061 ggccgaccac ccggccttcg agccatttgc tcatggcagg cgcacgatta tagcggccgc
     3121 tgccggccga actaggcgac ggctgccgct ttcgcgcccc tgcgcttttc ccaggcttcc
     3181 tgcgccgcgc cgagaatgcc cttgggcatg aagatgatga acagcaccag catcagcccg
     3241 tagagcgtgg tgtccaggcc gtggatgtcg tggccgagcg cgaggcgcag cacctcggcg
     3301 aggagcagcg tgaacgccgc gccgacggtc ggcccgagcc gcacgaacat gccgccggca
     3361 atggcgccga acaccatctg caggctgatg ccgatgcccg agacggtctc cgggttgacg
     3421 tagagctggt actgcgcgta gagcgcgccg cccatgcagg tcatcagcgc ggagatcacg
     3481 gtgacggtga gcttgagccg ggtgacgttc atgccgacgg aggccgcggc ctcctcctcc
     3541 tggctgatcg cctgcagcgc aagccgcgcc atcgagcggt cgacgcgcgt ccagatccac
     3601 agccccgcca gccagaaacc gaagatgatg tagaaccaca ccagcttgga ggagaactgc
     3661 agcgcggaaa gcgaccagga agcgtcggcc agagcggtca ccggggtcac gccgagcgag
     3721 ccgccggtct ggtcccggag cgcgacgatg atcaggcgcg ccacttccga gagcgcgagc
     3781 gtgaccagcg cgaaatagtg gcccacgatc ttgaagcgaa agcacgggta gccgatcaaa
     3841 atcgccacca cgcccgccac gccgagcgcg agcggaatgc cgaaccaggg cgaaagcccg
     3901 aagtggttcc agcccatcac caccacgtac gcgccgatgc caaggaacgc gccgtggccc
     3961 aggctcacca gcccgaggcg ccccatcacc gcccagccgg tatacacgaa ggcccacagc
     4021 agcgtcatga tcaagaggtg cagcgggtag ttgcccatgc ccgcgagcgg cgccgtggcg
     4081 agcagcgcca ggccgatcca gcgcagcgcg ttgcccacgt tacttgcctc cgagaaggcc
     4141 ctgaggccgc cagaacatca tcgcgatgaa gaacaggaac gcgaatacgt agccccattc
     4201 gatgtgcagg atgtagccgc ccaccgagat gaactgggcg aagatgaacg cggcgatgaa
     4261 gccgccgagc atgttcccga gcccgcccaa cacgcagatc atgaaggtga tgggaccgaa
     4321 ggtgaggccg atcgcggggt ggatgtcgta ctgcagcacg agcaggcagg ccgcgagccc
     4381 cgccagtgcg ccgcccagcg ccgaagtgat cacgaagatg cggttcgcgt tcacccccat
     4441 cagcggcatg atggcgcggt cctgcgaaat ggcgcggatc gcggtgccga cgaaagtacg
     4501 tgtgaggaac aggtacacgc cgatcatgcc gccaagcgcc accaggaagg cgatgagccg
     4561 cgcgaagctg aggtacatgt ccccgaactg cagcgccggc aggttgacgc cgatgttgcg
     4621 gaactcgacg ccgaaggcga gcgtggctgc ggcctgcagg aaaaaaagca ccccgccggt
     4681 caccagcagc tggttgatcg gctcggcggc aagcaccggt cggatcacga actggtgcag
     4741 cagcgcgccg agcaccgcca ccgccaggat ggcgaccagc atcgccagcc acagcggcca
     4801 gccgaacacg ttgtgcgagt agtacaccgc atacatgccg atcatcacca gctcggcgta
     4861 gcagatccac accacgtcga tgacgccgaa gatcaggtta agccccagcg ccagcagcgc
     4921 cagcacgccg ccgagcagca gcccgttgat caccgcttcg aggagaaaga cgtcgaagaa
     4981 ttccatcgtg cctccgggcc tacagcccca ggtaagcctt cttgatcagg tcgctctcaa
     5041 gcatctgggc gctggtgccg gcgagcttga tgcgcccggt ttccagcaag taggcgcgat
     5101 tcacgacttt gagcacctgg cggatgttct gctcgacgat cagcaccgtg tagccctcct
     5161 tgcagatgcg ccgcaccaga tcgaatatcg attgcacgac caccggcgcg aggcctgcgg
     5221 agggctcgtc gagcagcagc agcttcgggc ccgacatcag cgcgcggccg atggcgcaca
     5281 tctgctgctc gccgcccgag agcgtgccgg cgacctgccc gcgccgctcg cgcatgcgcg
     5341 gaaagagcga gtacacgaac tcgagccgct cggcgaattt cgcgcgcgcc gccggcatga
     5401 acgcgcccat cctcaggttg tcctcgaccg agagcttggc gaacaggcgc cggttctcgg
     5461 gcacgtgcgc gatgccgctg gcgatcactt cgtgcgcggg gatcgccgcg agcggccgcc
     5521 cttccatgct gatctcgccc gagcgggggt caatcagctt cgagatcacg cgcagtagcg
     5581 tcgtcttgcc ggcgccgttc ggtccgatca ccgccaccga ttcgcccgcc ttcacctcga
     5641 ggctgacgtc gaacagcgcc tgaaagccgc cgtagcccgc gctgacgttc gagacgttga
     5701 gcatcagccg agcctcgcgg cgaccgtggc ctgcacgtcg ccggcgtcgc tgccgagata
     5761 cacctcgatc accttgcggt cgtgcgccac ctcgtccggg cgccccgcgg cgatcacctc
     5821 gccgtggtcg agcacgatgc agcggtcgac gacgcgcatc agcacgccca tgatgtgctc
     5881 gacccagatg atggtgatgc cgcgcttgtc gcggatcccg gcgagcatgt cggccgcctg
     5941 gtccatctcg ctgtggtcca gccccccgag cgactcgtcg gccagcagca gctgcggccg
     6001 cgtggcgagc gcgcgcgcga gctcgagctt cttgaggccc gccgcgccca ggccgtccac
     6061 gcgcgccgcc gggtccgcgc tcagcccgac caggccgagc gcctcgccgg cctgcgcgaa
     6121 ggcctgctcg cgcgagggac gctcggcctg cccgtagaag gcggccagcg cgacgttctc
     6181 gaggatcgac aggcgccgga aggggcgggg gatctggaag gtgcgcgcga tgccgtagtg
     6241 cgcgacgcgc gccgcgctct gcccggcgat ctcgcggtcg cggaagcgga tcgagcccgc
     6301 ggtgggcgcg agcgcgccgg cgatcaggtt gaaggtggtg ctcttcccgg agccgttcgg
     6361 gccgatcagc ccgaggatct cgccttcctc gagctcgaag gcgacgcggt tcaccgcggt
     6421 aaatccgccg aactgcttgg tcaggtcggt gacggtgagc attgcgggca gcgtcggccg
     6481 gctccctccc ccgctgcggg ggagggaggg tgccggcctc gcgttcgcgt taccggcggt
     6541 aagccgagct cttcgggatc ggcagcagcg ggtcggcggt cttgatcgcc gtcggccaga
     6601 cgatcttggt cgaacccccg atgtactgca tcaccacggg cgaggagcgc tcgttctggc
     6661 cggccatctc gcttcccggc gcatagaact tgaccccgta gccctggatc gtgccgccca
     6721 ccggaatgtc cgtttcaagc gcggccttgc gcagcgcctc gggatcgacg ccgccgtact
     6781 tcttgatcgc gcgcggcagc acgtcggtga agaa
//
LOCUS       DBNHKE010000166         4769 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_980942, whole genome shotgun sequence.
ACCESSION   DBNHKE010000166 DBNHKE010000000
VERSION     DBNHKE010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_980942"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="AC16O9_11415"
     CDS             complement(<1..234)
                     /locus_tag="AC16O9_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR6517263.1"
                     /translation="MSERDVDRQLVARAQRGDKHAFGLLVEKYQRKLGRLLARFIRDP
                     AEVEDVTQEAFIKAYRALPAFRGDSAFYTWLYRI"
     gene            complement(352..792)
                     /locus_tag="AC16O9_11420"
     CDS             complement(352..792)
                     /locus_tag="AC16O9_11420"
                     /inference="COORDINATES: protein motif:HMM:NF018908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HIR6517264.1"
                     /translation="MAALRLELRASRALAGVLALVHGAGAACLAVFVPGYPGGVLALL
                     VLALGVASARKRALLRASGSVRALELGAGGAAILELVDGRRLAARVGERRHVGPWWVV
                     LPVQGESRCSLVVVRDMLPPPAFRSLRLWALWGRVPLAPPQHAA"
     gene            complement(798..2030)
                     /gene="fabF"
                     /locus_tag="AC16O9_11425"
     CDS             complement(798..2030)
                     /gene="fabF"
                     /locus_tag="AC16O9_11425"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR6517265.1"
                     /translation="MSRRRVAVTGLGIVCPVGNTVEAAWGNLVAGKPGIGPITRFDAT
                     LFACRIAGEVRGFDVSAYLPPKEARHMDVFIHYGLAAAIQAWKDCGLQVTDANAERIG
                     VNMGSGIGGLPRIEETHAELMQRGPRRISPFFVPASIINMISGHLSILYGMKGPNLAV
                     VTACTTGLHAVGQAARMIEYGDADVMVAGGAEATISPLGVGGFAAARALSTRNDDPAT
                     ASRPWDRDRDGFVLGEGAGALVLEEFEHAKARGARIYCEVTGFGMSGDAFHMTAPDLD
                     GPRRCMQAALANARISPDQVQYLNAHGTSTPLGDKNETEAVKKAFGAHAHKLVVNSTK
                     SMTGHLLGGAGGIESVFTVLAVHKQVSPPTINIFNQDPECDLDYCANTARDLKIQNAV
                     KNNFGFGGTNGTLVFARV"
     gene            complement(2044..2283)
                     /gene="acpP"
                     /locus_tag="AC16O9_11430"
     CDS             complement(2044..2283)
                     /gene="acpP"
                     /locus_tag="AC16O9_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6517266.1"
                     /translation="MENVEQRVRKIIAEQLGVNESEIKNESSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAIDYVKSHSKS"
     gene            complement(2349..3089)
                     /gene="fabG"
                     /locus_tag="AC16O9_11435"
     CDS             complement(2349..3089)
                     /gene="fabG"
                     /locus_tag="AC16O9_11435"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR6517267.1"
                     /translation="MLQDKLALVTGASRGIGHAIALELGRQGATVVGTATTEQGAAAI
                     GQALVQAGAKGAGRVLNVRDAAQGDALVAELQKDLGEVAILVNNAGITRDNLALRMKD
                     AEWDDVLDTNLKAVFRLSRAVMRGMMKARWGRIINITSVVGASGNAGQANYAAAKAGV
                     VGLTKSLARELGSRGITVNCVAPGFIDTDMTRALSEAQRSALLAQIPLGRLGETADIA
                     AAVAYLASPAAGYVTGCVLHVNGGMYMG"
     gene            complement(3092..4027)
                     /gene="fabD"
                     /locus_tag="AC16O9_11440"
     CDS             complement(3092..4027)
                     /gene="fabD"
                     /locus_tag="AC16O9_11440"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR6517268.1"
                     /translation="MKLAMVFPGQGSQAVGMLRAYAGLPGVDEVCRQAGDALGADFLA
                     LLDAGPQESLNLTVNTQPAMVTAGYAAFRAWRELGGPAPEFVAGHSLGEYTALVAAGS
                     IEFAQCLPLVRFRAQAMQQAVPEGQGAMAAILNLDDAAVRAACAEAAQGQVVDAVNFN
                     APGQIVIAGHKEAVTRAMELCKAKGAKRALPLPVSAPFHSSLMRPAAERLQGYLAGVE
                     VRAPACQLINNVDVRIETQPEAIKDALVRQAASPVRWVETVQQMRAHGVTHVLECGPG
                     KVLAGMVKRIDGALQSLALADKASLEQALATLKGV"
     gene            complement(4032..>4769)
                     /locus_tag="AC16O9_11445"
     CDS             complement(4032..>4769)
                     /locus_tag="AC16O9_11445"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR6517269.1"
                     /translation="DLIIVATSTPDFVFPSTACLLQAKLGIRNFPAFDVQAVCSGFVY
                     ALATADNFIRAGMSRRALVVGAEVFSRILDWNDRGTCVLFGDGAGAVVLAAAEQPGLH
                     ASVLRADGSHADILSVPGNVCGGRIVGSPYLQMQGSQVFKFAVKVLDEVARETLAAAG
                     MQLADVDWLIPHQANVRILEATARKLGLPLERLVVTVDHHGNTSAASVPLALDEYVRA
                     GKIRAGHRVMMQGVGGGFTWGATLATM"
BASE COUNT          749 a         1757 c         1517 g          746 t
ORIGIN      
        1 aatgcggtac agccacgtat agaacgcgct gtcgccgcga aacgccggaa gcgcccgata
       61 agccttgatg aacgcctcct gcgtcacatc ctccacctct gccggatcgc ggatgaaccg
      121 cgccagcagg cggccgagct tgcgctggta cttctccacc agcagcccga aagcgtgctt
      181 gtcaccgcgc tgcgcccgcg ccaccagctg tcggtcgaca tcgcgctcag acatcgccgg
      241 cgcccgccgc ggctccggcc cgggcagcat acccgccccg tccccggatg caacgcctct
      301 ctgccacaga cacggcacgc cggaaatagt tcgcccgcat acgcggcgtc atcaggccgc
      361 gtgctgcggc ggcgccaacg gcacccgacc ccacagcgcc cacagacgca aggaccggaa
      421 cgccgggggc ggcagcatgt cgcgcaccac cacgagactg catctcgatt cgccctgcac
      481 gggcagtacg acccaccacg gccccacgtg gcggcgctcg cctacgcgcg ccgcgagacg
      541 ccgtccgtcg accagctcga gtatcgcggc gccgcccgcg ccgagttcca gcgcgcgcac
      601 cgacccgctc gcgcgcagca gcgcccgctt gcgcgcgctc gccacgccca gcgccaggac
      661 cagcagtgcg aggacacccc cgggataacc gggaacgaag accgccaggc aggcggcacc
      721 ggccccgtgg accagcgcca gcacccccgc tagcgcgcgc gacgcacgca gctcgaggcg
      781 cagcgccgcc atgaaattca gacgcgagcg aacaccagcg taccgttggt gccgccaaaa
      841 ccgaagttgt tcttgactgc gttctggatc ttcagatccc gcgccgtgtt ggcgcaatag
      901 tccagatcgc actcggggtc ctggttgaag atgttgatgg tgggtggaga aacctgcttg
      961 tgcacggcga ggaccgtgaa taccgactcg atgccgccgg ccccaccgag caggtggccg
     1021 gtcatggact tggtggagtt caccaccagc ttgtgcgcgt gcgcgccgaa cgccttcttg
     1081 accgcctcgg tctcgttctt gtctcccaac ggcgttgaag tgccgtgcgc gttcagatac
     1141 tgcacctgat cgggggatat gcgtgcgttg gcgagcgccg cctgcatgca gcgcctgggc
     1201 ccgtcgagat ccggcgccgt catgtggaac gcgtcgccgc tcatgccgaa gccggtaact
     1261 tcgcaataga tgcgtgcacc gcgcgccttg gcgtgctcga attcctccag caccagtgcg
     1321 ccggcgcctt cgcccaggac gaacccgtcg cggtcccggt cccaggggcg gctcgccgtc
     1381 gccggatcgt cgttgcgcgt cgacagggcc cgtgcggcgg caaatccgcc cacgccgagc
     1441 ggcgagatcg tggcctcggc cccgcccgcc accatcacgt cggcgtcacc gtactcgatc
     1501 atgcgcgccg cctggcctac ggcgtgcagc cccgtggtgc aggccgtaac caccgccagg
     1561 ttggggcctt tcatgccgta taggatcgac aggtgcccgg agatcatgtt gatgatcgag
     1621 gccgggacga agaacggcga aatgcgccgc gggccgcgct gcattagctc ggcgtgcgtt
     1681 tcctcgatgc gcggcaggcc gccgatgccc gatcccatgt tcacgccgat gcgctcggcg
     1741 ttggcgtccg tcacctgcag gccgcagtcc ttccaggcct ggatcgccgc cgccagcccg
     1801 tagtggatga acacatccat gtgccgtgcc tctttcggcg gcaggtacgc ggacacgtcg
     1861 aagccccgca cctcgcccgc aatgcggcag gcgaaaagcg tcgcgtcaaa gcgcgtgatg
     1921 ggcccgatac cgggtttgcc ggcgaccagg ttcccccagg cggcctctac ggtgttgccg
     1981 accgggcaga cgatcccgag cccggtaacg gcgactctgc ggcgtgacac ttagcgcgcg
     2041 gcgttacgac ttggaatgcg acttcacgta gtcgatggcc tgctgcaccg tggtgatctt
     2101 ctcggcttcc tcgtcgggaa tctcggtctc gaactcctct tccagcgcca tcaccagctc
     2161 cacggtgtcc agagagtccg cgcccaaatc atcgacgaac gacgactcgt tcttgatctc
     2221 cgactcgttc accccgagct gctcggcgat gatcttcctg acgcgttgtt cgacgttctc
     2281 catgttctgc tccttcccta gtggggccaa gcggcggccc ggattttacc aaattggcgc
     2341 cctcgccctc accccatgta catgccgccg ttgacgtgca gcacgcaacc cgtgacgtag
     2401 cccgccgccg gcgacgccag ataggctacc gcggccgcga tgtcggcggt ttcgccgagc
     2461 cggccgagcg gaatctgcgc cagcagcgcg ctgcgctgcg cctccgagag cgcccgcgtc
     2521 atgtccgtgt cgatgaatcc gggggcaacg cagttcaccg tgatgccccg gctgccgagc
     2581 tcccgcgcga gcgacttggt cagacccacg acgccggcct tggcggccgc gtagttcgcc
     2641 tggcccgcgt tgcccgaggc gccgaccacc gacgtgatgt tgatgatgcg gccccagcgc
     2701 gccttcatca tgccgcgcat cacggcgcgc gacagccgga acaccgcctt caggttggtg
     2761 tcgagcacgt cgtcccattc ggcgtccttc atgcgcagcg cgaggttgtc acgggtgatg
     2821 ccggcgttgt tcaccaggat ggcgacttcg cccaggtcct tctgcagctc cgccaccagc
     2881 gcatcgcctt gcgcggcatc gcgaacgttc agcacacgcc ccgcaccctt ggcgccggcc
     2941 tgcaccagtg cctggccgat ggcggcggcg ccttgctcgg tagtggccgt acccacgacc
     3001 gtcgcgccct gccgtccgag ctcgagcgcg atcgcgtggc cgatgccgcg cgaggcgccg
     3061 gttaccaggg cgagcttgtc ctgcagcatc gtcagacccc cttcagagtt gccagagcct
     3121 gctcgagcga tgccttgtcc gccagcgcca ggctctgcag cgcaccgtca atgcgcttca
     3181 ccatgcccgc cagcaccttc ccggggccgc actccagcac gtgcgtcacg ccgtgggcgc
     3241 gcatctgctg caccgtctct acccagcgca ccggcgacgc cgcctggcgc accagcgcat
     3301 ccttgatcgc ttcaggctga gtctcgatgc gtacgtccac gttgttgatc aactggcagg
     3361 ccggtgcgcg cacctcgacg ccggcaaggt agccttgcag ccgctctgcc gccggccgca
     3421 tcaggctgga atgaaacggt gcgcttaccg gcagcggcag cgcgcgcttg gcgccctttg
     3481 ccttgcacag ctccatcgcc cgcgttaccg cttccttgtg gccggcgatc acgatctggc
     3541 ccggcgcgtt gaagttcacc gcgtcgacga cctgaccctg cgccgcctcg gcgcaggctg
     3601 cgcgaaccgc ggcgtcgtcg aggttgagga ttgccgccat cgccccctgc ccctcgggca
     3661 ccgcctgctg catcgcttgc gcgcggaagc gcaccagcgg caggcactgc gcgaattcga
     3721 tcgatcccgc cgcaaccaga gccgtgtact cgcccaggct gtggcccgcg acgaactcag
     3781 gtgccgggcc gccgagctcg cgccacgcgc gaaacgccgc atagccggcg gtcaccatgg
     3841 cgggctgcgt gttcaccgtg aggttgaggg attcctgcgg gcctgcgtcg agcaacgcga
     3901 ggaaatccgc gcccagcgca tcgccggcct gccggcacac ctcgtccacg ccgggcaacc
     3961 cggcataagc gcgcagcatg ccaacggctt gcgagccttg ccccggaaac accatcgcca
     4021 gcttcatctc gtcacattgt cgccagagtg gcgccccacg tgaagccacc gcccacgccc
     4081 tgcatcatca cccggtggcc ggcgcggatc ttgccggcgc gcacgtactc gtcgagggcg
     4141 agcggcacag aagccgccga cgtgttgccg tgatggtcca cggtcaccac cagccgctcg
     4201 agcggcaggc cgagcttgcg cgcggtggcc tccaggatac gcacgttggc ttggtgcgga
     4261 atgagccagt cgacgtccgc aagctgcatg ccggcggcgg cgagggtctc gcgcgccacc
     4321 tcgtcgagca ccttcaccgc gaacttgaac acctggctgc cctgcatctg aagatagggc
     4381 gagcccacga tgcgtccgcc gcacacgttt cccggcaccg acaggatgtc ggcatggctg
     4441 ccgtcggcgc gcagcacgga ggcgtgcagc ccgggctgct cggcagcggc cagcaccacc
     4501 gcgccggcgc cgtcgccgaa gagaacgcag gtgccgcggt cgttccaatc gaggatccgc
     4561 gagaacacct cggcgcccac gaccagcgcg cgccggctca tgccggcgcg aatgaagttg
     4621 tcggctgtgg cgagcgcgta gacgaagccg ctgcacaccg cctgcacgtc gaacgccggg
     4681 aagttgcgga tgccgagctt cgcctgcagc aggcaggccg tgctcggaaa cacgaagtcg
     4741 ggcgtcgagg tggcgacaat gatcaggtc
//
LOCUS       DBNHKE010000167        12313 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_134806, whole genome shotgun sequence.
ACCESSION   DBNHKE010000167 DBNHKE010000000
VERSION     DBNHKE010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_134806"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..476
                     /locus_tag="AC16O9_11450"
                     /pseudo
     CDS             69..476
                     /locus_tag="AC16O9_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
     gene            complement(604..2118)
                     /gene="ilvA"
                     /locus_tag="AC16O9_11455"
     CDS             complement(604..2118)
                     /gene="ilvA"
                     /locus_tag="AC16O9_11455"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR6517270.1"
                     /translation="MKREDYLERILRARVYEVAIESPLELAPALSRRLGNRLLLKRED
                     LQPVFSFKLRGAYNKMANLPPERLKKGVIAASAGNHAQGVALAAQKLGCRAVIVMPVT
                     TPRIKVDAVAARGAKVVLHGDSYDEAYAHAMLLKRRRGLAFVHPYDDPEVIAGQGTIG
                     MELLRQSARPIDAVFVAVGGGGLIGGIGAYVKHLRPGVKVIGVEPDDADAMTRSLKAG
                     RRVKLEHVGLFADGVAVKQVGKETFRLARKHIDEMVLVDTDEICAAIKDVFEDTRVVL
                     EPAGALAIAGAKAWVERHGARGKTLVAVACGANTNFDRLRFVAERAEVGEHREAVLGV
                     TIPEQRGSFRRFCRALGGRNITEFNYRMADPREAHIFVGVTVQDRAETGRILRGLRRA
                     GFRTLDMSTDEVAKGHMRHMIGGHSALARDELLYRFEFPERPGALMKFLTAMNPSWNI
                     SLFHYRNYGADYGSILVGLQVPRREMGDFRRFLARLGYPASDETRNPAYRLFLR"
     gene            2142..2549
                     /locus_tag="AC16O9_11460"
     CDS             2142..2549
                     /locus_tag="AC16O9_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR6517271.1"
                     /translation="MEHLLSPQGGLAAVLVASFVAATLVPLSSEAVLFGYLKLHPEHA
                     ALAVALATLGNTAGGMTTYLIGRLLPQRLPEARALRWLRRYGAPATFFAWLPFVGDAL
                     SAAAGWLRVNWLAALGCMAAGRLARYLAIAALS"
     gene            complement(2557..2928)
                     /locus_tag="AC16O9_11465"
     CDS             complement(2557..2928)
                     /locus_tag="AC16O9_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6517272.1"
                     /translation="MKAKKTERGMVLTLGDVLFDTGRSDLKPGALRTLEQLTAFLTEN
                     PERNAIIEGHTDSVGSQAYNQRLSEERAQSVQNALIERGIAPARLSAVGFGPTKPVVG
                     NDTAAGRQQNRRVEIILPDVK"
     gene            complement(3184..3504)
                     /locus_tag="AC16O9_11470"
                     /pseudo
     CDS             complement(3184..3504)
                     /locus_tag="AC16O9_11470"
                     /inference="COORDINATES: protein motif:HMM:NF025704.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
     gene            complement(3545..3931)
                     /locus_tag="AC16O9_11475"
     CDS             complement(3545..3931)
                     /locus_tag="AC16O9_11475"
                     /inference="COORDINATES: protein motif:HMM:NF025704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HIR6517273.1"
                     /translation="MNEPISARRRAAGAGLLAALLLGACATVEPLPAAEIAAAQAAIT
                     QAESAGAKHAAPVELLAAREKLEKADVAVREERFGDARRLAEAAEADALLADRKARAA
                     KAQSAADELVRSNELLRIEAGRNAPR"
     gene            complement(4055..6229)
                     /locus_tag="AC16O9_11480"
     CDS             complement(4055..6229)
                     /locus_tag="AC16O9_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6517274.1"
                     /translation="MSLRLEDLNPEQLAAVTLPAGHALILAGAGSGKTRVLTTRLAWL
                     LQTGAVTPAGVLAVTFTNKAAREMLARLTAMLAVNPRGMWIGTFHGLCNRMLRAHHRE
                     AGLPREFQILDTQDQLAAVKRVLRALNVDEDRYPPRDVQYFIMGRKEEGLRARDALAG
                     DDTSRRYAEFYDAYDEQCRREGVVDFAELLLRSFELLSRNELLREHYRARLRHLLVDE
                     FQDTNRLQYRWLKLLAGEGASLFAVGDDDQSIYAFRGANVGNMAEFEREYPGARVRLE
                     QNYRSQGNILDAANALIAHNAKRLGKNLWTAAGAGEPLRVFEAQSDADEARWMVEEIQ
                     ALKREGTRLNEIALLYRSNAQSRVLEHALFTAGLPYRVYGGLRFFERAEVKHALAYLR
                     LVATPADDNAFLRVVNFPARGIGARTLEALQDAAQAAGRNLYETALASPGKVAPFIAL
                     IESLREACAGLTLAERVEQVVAKSGLMEHYRREREGAERLENLSELVNAAAAFSAEDA
                     AVESDEDTDPLTAFLTHAALEAGEHQAGEGQDALQLMTMHSAKGLEFNAVFVSGLEEG
                     LFPHEQSVMEKDGLEEERRLMYVALTRARRRLYLSFAQTRLLHGQTRYNLPSRFIDEL
                     PQGLLKWLTPRFEHKRKPSTFTAGAFTRAPKRDPRFGGAPAGTGGAPSGFRIGQNVLH
                     AKFGAGVIVDAEGQGTDARVQVNFGREGVKWLAVAVAKLQAA"
     gene            complement(6261..7211)
                     /locus_tag="AC16O9_11485"
     CDS             complement(6261..7211)
                     /locus_tag="AC16O9_11485"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6517275.1"
                     /translation="MPRALLAVLVLAASLAAQAQSWPSKPIRLVVPYPPGGSTDVTAR
                     TLAERVSPALGQPLVVENRAGAGGNIGIEHAAKSDADGYTVLIAPDFVASAPHVYKLN
                     YDPMTQLVPVIQLTRQPVVLAVHPSLGVQSVAELVALAKAKPGLAYATSGAGSQQHIA
                     AEWFAKLAGIQLTHVPYKGGGQAIADFIGGQVPIASLGSTPVIPHHKSGKVKIIAQTT
                     KSRAPSLPEVPTYEQAGMAGLVIDQWLGVFVPAGTDAQVVRRLAAEFDKALAEPAVRE
                     RLANAALEAVGGSTEDFAQLVRANFETYRKLVADLNIRVN"
     gene            complement(7224..8102)
                     /locus_tag="AC16O9_11490"
     CDS             complement(7224..8102)
                     /locus_tag="AC16O9_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6517276.1"
                     /translation="MKRVLAIVGAALLAATAPARAAPAAVVEGVQMPAWVERAGIGAA
                     RRQPVAPGMELRGGDELRTGVGARIYVKLGEGSLVKLGENASLRILEVAPERGGLFKA
                     ALHVLEGAFRFTTDLLAQARRRQVSIRVANVTAGIRGTDLWGKSVPDKQIVCLIEGRI
                     EVGAEGEAPVVMDEPRQFYQRVKGATQPVGFVDATQLAQWARETEIEEGRGAARSGGN
                     WSVTLARADTQKAALEVYDRLRDAGYAAEIFPVKSGAGHEYRVRISRLSTKADAEALV
                     DQLRGTAGVEDPQVSN"
     gene            8194..10743
                     /gene="leuS"
                     /locus_tag="AC16O9_11495"
     CDS             8194..10743
                     /gene="leuS"
                     /locus_tag="AC16O9_11495"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR6517277.1"
                     /translation="MQPHYDPRSVEQEAQAFWKARGSFRCLEDKARPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRHYRMRGFNVLQPMGWDAFGLPAENAAMANKVPPAKWTY
                     DNIAYMKRQLQALGFALDWERELATCRPEYYKWNQWLFTRLFRKGLAYKKTGVVNWDP
                     VDQTVLANEQVIDGRGWRTGALIEKREIPMYFLRITAYADELLGALETMDGWPEQVKL
                     MQKNWLGRSEGVRIGFPYQIEGKARTLWVFTTRADTLMGTTFMAVAAEHPIAAFAAKN
                     DPKLAAFVEECKRGAVMEADLAQLEKKGMPTGLFCTHPVSGKPLPVWVGNYVLMGYGE
                     GAVMGVPAHDERDYAFALKYGLPIECVIRHPAGDAVPAPWQAAYAEYGTCINSGKYDG
                     LDYAAAVDAIAADLKAKNLGEKQVQWRLRDWGISRQRYWGCPVPIVHCPACGDVPVPD
                     DQLPVLLPEHLVPDGTGNPLAKLPSFFETACPSCGKPARRETDTMDTFVDSSWYFARF
                     ACPDNDAAMLDARANYWMPVDQYIGGIEHAILHLLYSRFFTRAMKAEGLLEVDEPFTN
                     LLTQGMVLAESYYSEAGGRREWINPAEVEVTRDAKGRILAAKTKDGREAVYDGIGTMS
                     KSKNNGVDPQALIDKYGADTARFYIIFASPPTNTLEWSDESVEGSFRFLRRVWAYAQK
                     YAAGPAPAGEHKATRHEVHSVLRQANYDLGKHQFNTVASAAMKILNALDAIPGRSAVS
                     EEGLSILLRLLAPVTPHLSHHLWRALGFGEDVLAAPWPEPDAGALKQDVTEYVLQVNG
                     KTRGKLQAPASADRGTIESLVTASEVVKKYISDQVIKKIIIVPGRLINVVV"
     gene            10770..11231
                     /locus_tag="AC16O9_11500"
     CDS             10770..11231
                     /locus_tag="AC16O9_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR6517278.1"
                     /translation="MLLAACGFQLRGAADMPFETLYVPGATGGIALELKRHIQSGSAT
                     RVVDDPKAAQAQLQFSEETRSKEILALSAAGRVREYRLIYRVGFRVDDGKGANFVPPS
                     TVTLTRDVTYDDAVALAKETEEQLLFRDMQADMVQQIMRRLAAALPPAAAN"
     gene            11231..12235
                     /gene="holA"
                     /locus_tag="AC16O9_11505"
     CDS             11231..12235
                     /gene="holA"
                     /locus_tag="AC16O9_11505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6517279.1"
                     /translation="MQLRAEQLEAQLAKTLAPLYVIHGDEPLIALEAADAVRAAARKR
                     GLAEREVFIAERGFDWSALAHAGASRSLFGGGKLIELRIPGGKPGTAGAETIVEHCKR
                     LDAEMLTLVSLPRLDRAGQSSAWFGALAAAGVVVEIWPVERARLPQWIGERLARQQQK
                     APREVLEFLAERVEGNLLAAHQEVQKLALLAPPGELALETVEDAVASVARYDPYAAAE
                     ALVSGDAARYARVLQGLRGEGESPTFVLFAVSSALFVLRGLAEGKSAERLFGEQRLYN
                     KPLQRAVQAAARRYTPATLDAALAQAAFIDRVAKGVARGDPWDQFAALGLKLAGGSKA
                     "
BASE COUNT         1736 a         4387 c         4315 g         1875 t
ORIGIN      
        1 tcgctccatg gcgaaaaagc gaacgtgccc gccttatcgc tgcgcgcgag gcggtcgacg
       61 cgccgacgct ggaggcctgg cgccgtgcca tcgacgcgca cctcgagcgc ggcttcccgg
      121 atctggcgaa ggcgcgcgtc gcgttctgct ggccggtccg caaggagtat gacgcgcgcc
      181 acctcgcgcg ccggctgcgc gcatcgggcg cggtgacggc gctcccggtg gtgctggcgc
      241 ccaagacgcc gctcgcgttc cgcgagtggt accccggggt cgagctggcc gaaggaccgc
      301 tcggcatccc gtatcccgtc ggctcgcgcg aaatcgtgcc gcaggccgtg ctcctgccga
      361 tgaacggctg ggacgcgcaa ggctaccggc tgggctacgg cggcggcttt ttcgatcgca
      421 ccctcgccgc gctggcggcg aagccccaag tgatcggcat cgcctacgag ctcgcgcgca
      481 tcgccaccat ccatccgcag gcgtgggaca tccccgtgga ctacctggtg accgagcgcg
      541 gcatctaccg gcgcgacgcc ggcgagcttg ctttcctcgg cgcgcccgag ccgcagggcg
      601 cggctagcgc aggaacagcc gataggccgg gttcctcgtc tcgtcgctcg ccggatagcc
      661 gaggcgcgca aggaagcggc ggaagtcgcc catctcgcgc cgcggcacct gcaggccgac
      721 caaaatcgag ccgtagtcgg cgccgtagtt gcggtagtgg aacaggctga tgttccagga
      781 ggggttcatc gccgtgagga acttcatcag cgcgccgggg cgttcgggaa actcgaaccg
      841 gtagagcagc tcgtcgcgcg cgagcgccga gtgcccgccg atcatgtggc gcatgtggcc
      901 cttcgccacc tcgtcggtgg acatgtcgag cgtgcgaaaa cccgcgcggc gcaggccgcg
      961 caggatgcgg ccggtttccg cgcggtcctg caccgtgacg ccgacgaaga tgtgcgcctc
     1021 gcgcgggtcc gccatgcggt agttgaactc ggtgatgttg cgcccgccga gcgcgcggca
     1081 aaagcgccgg aagctgccgc gctgctcggg gatcgtcacg ccgaggaccg cctcgcggtg
     1141 ctcgcccacc tcggcgcgct cggccacgaa gcgcaggcgg tcgaaattgg tattggcgcc
     1201 gcaggccacc gcgaccagcg tcttgccgcg cgcgccgtgg cgctcgaccc aggccttggc
     1261 gccggcgatc gccagcgcgc cggcgggctc gagcaccacg cgcgtgtcct cgaacacgtc
     1321 cttgatcgcg gcgcagatct cgtcggtatc gacgagcacc atctcgtcga tgtgcttgcg
     1381 cgcgaggcga aaggtctcct tgcccacctg cttcaccgcc acgccgtcgg caaacaggcc
     1441 cacgtgctcg agcttgacgc gccgcccggc cttgagcgag cgcgtcatgg cgtcggcgtc
     1501 gtcgggctcg acgccgatca ccttgactcc cgggcgcagg tgcttcacgt aggcgccgat
     1561 gccgccgatc agcccgccac cgcccaccgc cacgaacacg gcgtcgatcg gccgcgcact
     1621 ctggcgcagc agctccatgc cgatggtgcc ctggccggcg atgacctccg gatcgtcgta
     1681 gggatgcacg aaggcgaggc cgcgcctgcg cttgagcagc atggcgtgcg cgtaggcctc
     1741 gtcgtaggaa tcgccgtgca gcacgacttt cgcgccgcgc gcggccacag cgtcgacctt
     1801 gatccgcggc gtggtcaccg gcatcacgat caccgcgcgg cagccgagct tctgcgcggc
     1861 gagcgccacg ccctgcgcgt ggttgccggc gctcgcagcg atcacgcctt tcttcagccg
     1921 ctcgggcggc aggttggcca tcttgttgta ggcgccgcgc agcttgaacg agaacaccgg
     1981 ctgcaggtcc tcgcgcttga gcagcagccg gtttcccagg cgccgcgaga gcgccggcgc
     2041 gagctccagc ggcgactcga tcgccacctc atagacccga gcgcgcagga tgcgctcgag
     2101 atagtcttcg cgtttcaccg aaaaagccta gcacaccgct cttggagcac ctgctctcgc
     2161 cgcaaggcgg cctcgccgcc gtgctcgtcg ccagcttcgt cgcggcgacg ctggtgccgc
     2221 tgtcgtccga ggcggtgctg ttcggctacc tgaagctgca tccggagcac gcggcgctcg
     2281 ccgtggcgct ggcgacgctc ggcaacaccg cgggcggcat gacgacctac ctcattggcc
     2341 ggctgctacc gcagcggctg cccgaggcaa gggcattgcg ctggctgcgc cgctacggcg
     2401 cgccggcgac gttcttcgcc tggctgccct tcgtcggcga cgcgctcagc gccgcggcgg
     2461 gctggctgcg cgtgaactgg ctcgccgcgc tcggctgcat ggccgccggg cgcctggcgc
     2521 gctacctggc gatcgcggcg ctgtcctagc gcgcgctcac ttcacgtcgg gcaggatgat
     2581 ctcaacgcgc cggttctgct ggcgccccgc cgccgtgtcg ttgccgacca ccggtttggt
     2641 ggggccgaag ccgacggcgc tcaagcgcgc cggcgcgatg ccgcgctcga tgagggcgtt
     2701 ctgcaccgat tgcgcgcgtt cctccgaaag acgctggttg tacgcctgcg atccgaccga
     2761 atcggtatgg ccttcgatga tcgcgttgcg ctccgggttc tccgtcagga acgcggtgag
     2821 ctgctccagg gtgcgcagcg cgccgggctt gaggtcggaa cgcccggtat cgaacagcac
     2881 gtcgcccagc gtgagcacca tgccgcgctc ggtcttcttc gccttcatct cctcaagctg
     2941 cgccgcgagg cgcttggcct gctcgtcggc cgcgcgcgcg gcggcggccg cggcctgcgc
     3001 ccgagcgagc tgctcctcgg cctgcttgcg ggcggctacg gcctgctcct gctgctcctg
     3061 ggcgcgcttc cttgcggcgt cggcttcggc ggcggaagcg agagcgcgct tctgcatttc
     3121 ctcggcctcg cgctcgcgca cctgcatcaa gatgcgctgg cgctcgagag cggcgctggc
     3181 caccgcctct tcgccggcca cgaccctgcc ggcttcgagg gccgtttcgg tcctgcgttt
     3241 cgccagatac gcgtggtgct ccacgatggc ggcatcttcg cccttgtcca gcgcggcctg
     3301 cgcgcgctgc aatgcgacgc gcgcgctttg cagctcgacg gtgccgccgc gcgccgcgta
     3361 ggcgttgttg acggcgcgct cgtagccggc gcgcgcttca tcgagcaggg cgttctgggt
     3421 gggtgccgtg gcgcacgcgg cgagcacagc cgcggcggcg atggccagcg cgctgttgcg
     3481 ggcgcaaccg ctgaaatttt tcatggcaga gtcctttggg gtcaggtatc agcgtgagcg
     3541 cacactatcg gggcgcgttg cgtccggcct cgatgcgcag aagctcattg ctgcgcacca
     3601 gctcgtccgc agcgctctgc gccttggccg cgcgcgcctt gcgatcggcg agcagggcgt
     3661 ccgcttccgc ggcctcggcg aggcggcgcg cgtcgccgaa ccgctcttca cgcaccgcga
     3721 cgtcggcctt ttccagcttc tcgcgcgccg cgagcagctc gacgggggcg gcgtgtttcg
     3781 cgccggcgct ttcggcctgg gtgatcgccg cctgcgcggc ggcgatttca gcggccggca
     3841 gcggttcgac agtcgcgcag gcgcccagaa gcagcgcagc gagcaggccg gcgccggctg
     3901 cccgcctgcg agcggaaatc ggttcgttca tggatgtttc ctctggaaat gcgatggggg
     3961 gtttcgcgcc cgcgccttac gccgcgaggt gcgacggatt ctactcagat tccggtgccc
     4021 gccgtctact actgcagcgt cgggcggcgg cgactcaggc cgcctgcagc ttggccaccg
     4081 ccaccgcgag ccatttcacg ccctcgcggc cgaagttcac ctgcacgcgc gcatcggtcc
     4141 cctggccttc ggcgtcgacg atgacgccgg cgccgaactt ggcgtgcagc acgttctggc
     4201 cgatgcgaaa gccgctcggc gccccgccgg tgcccgccgg cgccccaccg aagcgcgggt
     4261 cgcgcttggg cgcgcgcgtg aacgcccccg cggtgaacgt ggagggcttg cgcttgtgct
     4321 cgaagcgtgg cgtaagccac ttcagcagcc cctgcggcag ctcgtcgatg aagcgcgagg
     4381 gcaggttgta gcgcgtctgc ccgtgcagca gccgcgtctg cgcgaaagac aggtagaggc
     4441 gccggcgcgc gcgcgtgagc gccacgtaca tcaggcgccg ctcctcctcc agcccgtcct
     4501 tctccatcac gctctgctcg tgcggaaaca ggccctcttc cagcccgctc acgaacacgg
     4561 cgttgaattc caggcccttg gccgagtgca tggtcatcag ctgaagcgcg tcctggccct
     4621 cgcctgcctg gtgctcgccg gcttccagtg ccgcatgcgt gagaaacgcg gtgagcggat
     4681 cggtgtcctc gtccgattcg accgccgcgt cctcggcgct gaaggcggcc gccgcgttca
     4741 ccagctccga gaggttttcc aggcgctcgg cgccctcgcg ctcgcgccgg tagtgttcca
     4801 tgagcccgct cttggccacc acctgctcga ccctctccgc gagggtgagg ccagcgcacg
     4861 cctcgcgcag cgactcgatg agggcaatga agggcgccac cttcccgggg ctggcgagcg
     4921 cggtctcgta gaggttgcgg cccgcggcct gcgccgcgtc ctgcagcgcc tccagcgtgc
     4981 gcgcgccgat gccgcgcgcc gggaagttca ccacgcgcag gaacgcgttg tcgtccgcgg
     5041 gcgtggccac caggcgcagg taggcgagcg cgtgcttcac ctcggcgcgc tcgaaaaagc
     5101 gcagcccccc gtacacgcgg tacggcagcc cggcggtgaa cagggcatgc tccagcacgc
     5161 gcgactgcgc gttcgagcgg taaaggagcg cgatctcgtt caagcgcgtg ccctcgcgct
     5221 tgagcgcctg aatctcctcc accatccagc gcgcctcgtc ggcgtcggac tgcgcctcga
     5281 acacgcgcag cggctcgccc gcgccggccg cggtccacag gttcttgccc aggcgcttgg
     5341 cgttatgcgc aatgagcgcg ttggcggcgt ccaggatgtt gccctgcgag cggtagttct
     5401 gctcgagccg cacgcgcgcg cccggatact cgcgctcgaa ctcggccatg ttgccgacgt
     5461 tggcgccgcg gaaggcgtag atcgactggt cgtcgtcgcc gaccgcgaac aggctcgcgc
     5521 cctcgccggc gaggagcttc agccagcggt actgcaggcg gttggtgtcc tggaactcgt
     5581 ccaccagcag gtgacgcaag cgcgcacggt agtgctcgcg cagcagctcg tttcgcgaca
     5641 gcagctcgaa gctccgcagc agcagctcgg cgaagtccac cacgccctcg cgccggcact
     5701 gctcgtcgta ggcgtcgtag aactcggcgt agcgccgcga ggtgtcgtcg ccggcgagcg
     5761 cgtcgcgcgc gcgcaggccc tcttccttgc ggcccatgat gaagtactgc acgtcgcgcg
     5821 gcgggtagcg gtcctcgtcc acgttcagtg cgcgcagcac gcgcttcacc gcggcgagct
     5881 ggtcctgcgt gtcgaggatc tggaactcgc gcggcagccc ggcctcgcgg tggtgcgccc
     5941 gcagcatgcg gttgcagagg ccgtggaagg tgccgatcca catgccgcgc gggttcaccg
     6001 cgagcatcgc cgtcaggcgc gcgagcatct cgcgcgccgc cttgttggtg aaggtcacgg
     6061 cgagcacgcc tgccggcgtc acggcgcccg tctgcagcag ccaggcgagt cgcgtggtga
     6121 gcacgcgcgt cttgccgctg ccggcgccgg caaggatcag cgcgtggccg gcgggcagcg
     6181 tgacggcggc gagctgttcg gggttgaggt cttcgagtcg gagcgacaaa acactccgat
     6241 tatagtctgc gcgcgcgccc tcaattgacg cggatgttca ggtccgcgac gagcttgcga
     6301 taggtctcga agttcgcgcg caccagctgc gcgaagtctt ccgtgctgcc gccgacggcc
     6361 tcgagcgcgg cattcgccag gcgctcgcgc accgcgggct cggcgagcgc cttgtcgaac
     6421 tcggcggcca ggcgccgcac gacctgcgcg tcggtgccgg caggcacgaa cacgccgagc
     6481 cactggtcga tcaccaggcc cgccatgccg gcctgctcgt aggtcggcac ctcgggcagg
     6541 ctgggcgcgc gcgacttcgt cgtctgcgcg atgatcttca ccttgcccga cttgtggtgc
     6601 gggatcaccg gcgtcgagcc gagcgaggcg atcggcacct gcccgccgat gaagtccgcg
     6661 atcgcctgcc cgccgccctt gtagggcacg tgcgtcagct ggatgccggc gagcttggcg
     6721 aaccattccg cggcgatgtg ctgctgcgag ccggcgccgg aagtggcgta ggcgaggccg
     6781 ggcttcgcct tggccagcgc cacgagctcg gccaccgact gcacgccgag cgacgggtgc
     6841 accgcgagca ccaccggctg gcgcgtgagc tggatcaccg ggacgagctg cgtcatcggg
     6901 tcgtagttca gcttgtacac gtgcggcgcg ctcgccacga agtccggcgc gatcagcacc
     6961 gtgtagccgt cggcgtcgct cttcgccgcg tgctcgatgc cgatgttgcc gccggcgccg
     7021 gcgcggttct ccaccaccag cggctggccg agcgccggcg acacgcgctc ggcgagcgtg
     7081 cgcgcggtga cgtcggtcga gccgcccggc ggatagggca cgaccagccg gatcggcttc
     7141 gacggccagg actgcgcttg cgccgcgagc gacgcggcga ggacgaggac ggcgagtagc
     7201 gcacgcggca tggcagattg ctcctagttg gaaacttgcg gatcctcgac gccggcggtg
     7261 ccgcgcaact gatcaacgag cgcctcggcg tcggctttcg tgctcagccg ggaaatgcgc
     7321 acccggtact cgtgcccggc gcccgacttg acgggaaaga tctcggcagc gtagccggcg
     7381 tcgcgcagcc ggtcgtacac ctcgagcgcc gctttctgcg tgtccgcgcg cgcgagcgtc
     7441 acgctccagt tgccgccgct gcgcgccgcg ccgcggccct cctcgatttc ggtctcgcgc
     7501 gcccactgcg cgagctgcgt cgcgtcgacg aagcccacgg gctgggtcgc gcccttcacg
     7561 cgctggtaga actggcgcgg ctcgtccatg actaccggcg cctcgccctc ggcgccgact
     7621 tcgatcctgc cctcgatcag gcacacgatc tgcttgtccg gcaccgactt gccccacagg
     7681 tcggtgccgc ggatgccggc ggtgacgttg gcgacgcgga tgctcacctg gcgccggcgc
     7741 gcctgcgcca gcagatcggt ggtgaagcgg aatgcccctt ccaggacgtg cagcgccgcc
     7801 ttgaacaagc cgccgcgctc gggcgcgact tcaaggatgc gcaggcttgc gttctcgccg
     7861 agcttcacca ggctgccttc gccgagcttg acgtagatgc gcgcgcccac gccggtgcgc
     7921 agctcgtcgc cgccgcgcag ctccatcccg ggcgcaaccg gctggcgccg cgccgcgccg
     7981 atgccggcgc gctccaccca ggccggcatc tgcacgcctt ccaccaccgc ggccggcgcc
     8041 gcccgtgccg gcgcggtggc ggcaagcagc gcggcgccga cgatggcgag cacgcgtttc
     8101 atcgagatat ccatggccct gagcgcaagc ataaccgggt cttcgcccgg gggcgatgcg
     8161 ctaaaatccg cgccttccgc gcgtttcgcc acgatgcagc cccactacga tccccgcagc
     8221 gtcgagcagg aggcgcaggc cttctggaag gctcgcgggt ccttccgctg cctcgaggac
     8281 aaggcgcggc ccaagttcta ctgcctgtcg atgttcccct acccctcggg caagctgcac
     8341 atggggcacg tgaggaacta caccatcggc gacgtgctca cccgccacta ccgcatgcgc
     8401 ggcttcaacg tgctgcagcc gatgggctgg gacgccttcg ggctgcccgc agagaacgcg
     8461 gcgatggcga acaaggtgcc gccggcgaag tggacctacg acaacatcgc ttacatgaag
     8521 cgccagctgc aggcgctcgg cttcgcgctc gactgggagc gcgagctcgc cacctgccgg
     8581 cccgagtact acaagtggaa ccagtggctg ttcacgcgcc tgttccgcaa gggcctcgcc
     8641 tacaagaaga ccggcgtcgt caactgggat ccggtcgacc agaccgtgct cgccaacgag
     8701 caggtgatcg acgggcgcgg ctggcgcacc ggcgcgctca tcgagaagcg cgagatcccg
     8761 atgtacttcc tgcgcatcac cgcatacgcc gacgagctgc tcggcgcgct ggagacgatg
     8821 gacggctggc ccgagcaggt caagctgatg cagaagaact ggctggggcg ctcggagggc
     8881 gtgcgcatcg gctttcccta ccagatcgaa ggcaaggcgc gcacgctgtg ggtgttcacg
     8941 acgcgcgccg acaccctgat ggggaccact ttcatggcgg tcgcggccga gcacccgatc
     9001 gcggcgttcg cggcgaagaa cgatccgaag ctcgccgcct tcgtcgagga gtgcaagcgc
     9061 ggcgcggtga tggaagccga cctcgcgcag ctggagaaga aaggcatgcc gacggggctt
     9121 ttctgcaccc acccggtctc cggcaagccg ctcccggtgt gggtcggcaa ctacgtgctg
     9181 atgggctacg gcgagggcgc ggtgatgggc gtgccggcgc acgacgagcg cgactacgcc
     9241 ttcgcgctca agtacggcct gcccattgaa tgcgtgatcc gccatccggc gggcgatgcg
     9301 gtgcccgcgc cctggcaggc ggcgtacgcc gagtacggca cctgcatcaa ctcgggcaag
     9361 tacgatgggc tcgactacgc ggcggcggtc gacgccatcg cggccgacct gaaggcgaag
     9421 aacctgggcg agaagcaggt gcagtggcgc ctcagggact ggggcatctc gcgccagcgc
     9481 tactggggct gcccggtgcc gatcgtgcac tgccccgcgt gcggcgacgt gccggtgccc
     9541 gatgaccagc tccccgtgct gcttcccgag cacctggtgc ccgacggcac cggcaacccg
     9601 ctggcgaagc tgccttcatt cttcgagacg gcgtgcccgt cgtgcggcaa acccgcgcgg
     9661 cgcgagaccg acaccatgga cactttcgtc gattcgtcct ggtacttcgc gcgctttgcc
     9721 tgcccggaca acgacgcggc catgctcgac gcgcgcgcga actactggat gccggtcgac
     9781 cagtacatcg gcggcatcga gcatgccatt ctgcacctgc tctattcgcg cttcttcacg
     9841 cgcgccatga aggcggaggg gctgctcgag gtggacgagc cgttcaccaa tctcctcacc
     9901 cagggcatgg tgctcgccga gtcctactac agcgaggcgg gcgggcggcg cgagtggatc
     9961 aacccggccg aggtcgaggt gacgcgcgac gccaagggca ggatcctcgc ggcgaagacg
    10021 aaggacggcc gcgaggcggt gtacgacggc atcggcacca tgtcgaagtc gaagaacaac
    10081 ggcgtcgacc cgcaggcgct gatcgacaag tacggcgccg acacggcgcg cttctacatc
    10141 atcttcgcct cgccgccgac caacacgctg gaatggtcgg acgagtccgt cgagggctcg
    10201 ttccggttcc tcaggcgcgt gtgggcctat gcgcagaagt acgccgccgg gccggccccc
    10261 gccggcgagc acaaggccac gcgccacgag gtgcacagcg tgctgcgcca ggcgaactac
    10321 gacctcggca agcaccagtt caacacggtg gcctctgccg cgatgaagat cctcaatgcg
    10381 ctcgacgcga taccgggccg cagtgccgtc agcgaggaag gcctgtcgat cctgctgcgg
    10441 ctgctcgcac ccgtcacgcc gcatctcagc caccacctgt ggcgcgcgct cggcttcggc
    10501 gaggacgtgc ttgccgcgcc ctggcccgag cccgacgcgg gcgcgctcaa gcaggacgtg
    10561 accgagtacg tgctgcaggt gaacggcaag acccggggca agctgcaggc gcccgcgtcc
    10621 gccgaccggg gcacgatcga gtccctggtt acggcgagcg aagtggtgaa gaaatacatc
    10681 tcggaccagg tgatcaagaa gatcatcatc gtccccgggc gcctcatcaa cgtcgtcgtt
    10741 tgaggctcgc gctcgccacc gctgccgctt tgctcctcgc cgcctgcggc ttccagctgc
    10801 gcggcgcggc cgacatgccc ttcgagacgc tgtacgtgcc cggggccacc ggcggcatcg
    10861 cgctcgagct gaagaggcac atccagtcgg gaagcgcgac gcgcgtggtc gacgatccca
    10921 aggcggccca ggcgcagctg cagttcagcg aggagacgcg ctcgaaggaa atcctcgcgc
    10981 tcagcgccgc cgggcgcgtg cgcgagtacc gcctcatcta ccgggtcggc tttcgcgtcg
    11041 acgacggcaa gggcgcaaat ttcgtgccgc cgagcacggt cacgctgacg cgcgacgtca
    11101 cctacgacga cgcggtggcg ctcgccaagg agaccgagga gcagctgctg ttccgcgata
    11161 tgcaggccga catggtgcag cagatcatgc gccgcctcgc cgcggcgctg ccgcccgcgg
    11221 ccgcgaactg atgcagctgc gcgccgagca gctcgaggcg cagctcgcga agacgctcgc
    11281 gccgctctac gtcatccacg gcgacgagcc gctgatcgcg ctggaagcgg ccgacgcggt
    11341 gcgcgccgcg gcgcgtaagc gcggccttgc cgagcgcgag gtattcatcg ccgagcgcgg
    11401 cttcgactgg agcgcgctcg cgcacgccgg ggcgagccgg tcgctcttcg gcggcggcaa
    11461 gttgatcgag ctgcgcatcc cgggcggcaa gcccggcacc gcgggcgccg agacgatcgt
    11521 cgagcactgc aagcggctcg acgccgagat gctgacgctg gtcagcctgc cgcgcctgga
    11581 ccgcgccggc cagtcctcgg cgtggttcgg cgcgctcgcc gcggccggcg tggtggtcga
    11641 aatctggccg gtggagcgcg cgcgcctgcc gcagtggatc ggcgagcgcc tggcgcgcca
    11701 gcagcaaaag gcgccgcgcg aggtgctcga gttcctcgcc gagcgcgtcg aaggcaacct
    11761 gctcgccgcg caccaggagg tgcagaagct ggcgctgctc gcgccgccgg gcgagctcgc
    11821 gctggagacc gtcgaggacg cggtggcctc ggtggcgcgc tacgacccgt acgccgcggc
    11881 cgaggcgctg gtgtcgggcg atgccgcgcg ctacgcgcgc gtcctgcagg gcctgcgcgg
    11941 cgagggcgag tcgccgacct tcgtgctgtt cgcggtctcg agcgcgctgt tcgtgctgcg
    12001 cggcctggcc gagggcaaga gcgccgagcg cctgttcggc gagcagcgcc tgtacaacaa
    12061 gccgctgcag cgcgcggtgc aagccgcggc gcggcgctac acgccggcga cgctggatgc
    12121 ggcgctcgcg caggcggcgt tcatcgaccg cgtggcaaag ggcgtggcgc gcggcgaccc
    12181 ctgggaccag ttcgccgccc tggggttaaa attggccggt ggatcaaaag cttgacgtaa
    12241 aagcctatgt gcgcggcctg ggccaggcgg cgcgcgccgc cgcgcgcgtc ctcgcccgcg
    12301 ccgacaccgg cgc
//
LOCUS       DBNHKE010000168         4288 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_187933, whole genome shotgun sequence.
ACCESSION   DBNHKE010000168 DBNHKE010000000
VERSION     DBNHKE010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_187933"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..760
                     /locus_tag="AC16O9_11510"
                     /pseudo
     CDS             1..760
                     /locus_tag="AC16O9_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="complex I 51 kDa subunit family protein"
     gene            835..3006
                     /gene="nuoG"
                     /locus_tag="AC16O9_11515"
     CDS             835..3006
                     /gene="nuoG"
                     /locus_tag="AC16O9_11515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR6517280.1"
                     /translation="MPKLEIDGRPIEVAPGTPVIEAAKRLGIYVPHFCYHSKLSIAAN
                     CRMCLVEVEKAPKPMPACATPVSDGMKVWTHSPSAKKAQNGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSASRYQEEKRVVLRKDLGPLVAAEEMTRCIQCTRCVRFGEEI
                     GGVMELGMVNRGELAEIVAFVGRGVDSELSGNMIDLCPVGALTSKPFRYAARTWELSR
                     RRSVSPHDGLGSNLVVQVKQNEVLRVLPLENEAVNECWLSDKDRFSYEGLNAPDRLTR
                     PMARIDGRLQEVEWEVALERAVAGLADAGVLASPHATLEELYLAGRLGAADFRLRHGD
                     FSADGRRQGIPWLGMPIAELGSLDAVLVIGSFLRKDHPLLAHRLRQAAKRGAQIHVLH
                     SVDDDWLMPIASKTIVAPSAIPGAIAAFKDALAGAKNAAVLLGNFAQQHPQAAAIHAA
                     AQALGVKLGFFGEAANSVGGYVAGLPSGGGLPEALKKEALLLLNVEPALDCADPRAAA
                     RAKFIVNLTAWRSELGDVLLPIAPFTETAGTFINTEGRVQGFHACARPLGEARPAWKV
                     LRVLGTMLGRPGFEFDTIEQVRDACLSGRDVAALLGNGIDAPAGGAATPPTGIERIAE
                     VPIYFADPLVRRASSLQKTRDARAPRAWMNARLMQRLGLADGQPVRVRQGEGSAKLAA
                     ALDDRLPDDCVRIAAAHGSTAPLGPMFGSVQVEKLSAEKAA"
     gene            3007..4044
                     /gene="nuoH"
                     /locus_tag="AC16O9_11520"
     CDS             3007..4044
                     /gene="nuoH"
                     /locus_tag="AC16O9_11520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR6517281.1"
                     /translation="MDWRLALVDTAWNLAGIVAIVAPLFLAVAYLTLWERKLIGWIQI
                     RIGPNRVGPRGLLQPIADGVKLLLKEIIVPTNASTALFIVAPIMMLMPAMAAWAVIPF
                     APELVLADINAGLLYVLALSSLGVYGVIIAGWASNSKYAFLGAMRSAAQMVSYELAMG
                     FALVVVLMVSGSLNLSDIVAQQGRGRFADMGLTFLSWNWLPLLPMLVVYFVAGVAETN
                     RAPFDVVEGESEIVAGHMIEYSGMSFALFFLAEYMNMLLISALTAVMFFGGWQPPISS
                     PVFDAVPPILWLLAKIFVIVSMFLWIRATFPRYRYDQIMRLGWKVFIPLTLVWIAVIG
                     LWMQTPWSLWR"
     gene            4041..>4288
                     /gene="nuoI"
                     /locus_tag="AC16O9_11525"
     CDS             4041..>4288
                     /gene="nuoI"
                     /locus_tag="AC16O9_11525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR6517282.1"
                     /translation="MIARVTDFFRAFLLLELFKGLALTGRHLFARKITVQFPEEKTPQ
                     SPRFRGLHALRRYPNGEERCIACKLCEAVCPALAIHIES"
BASE COUNT          647 a         1456 c         1503 g          682 t
ORIGIN      
        1 tgcttatatc tgcggcgaag agactgcctt gctcgagtcg atcgaaggca agaaggggca
       61 gccgcgcttc aagccgccgt tcccggcaag ctacggcctg tacggcaagc cgaccaccgt
      121 caataacacc gagaccctcg ccgcggtgcc ctggatcatg cgccatggcg cggacgcgtt
      181 ccttgcgctg gggcggccga acaacggcgg caccaagatc ttctcggtca ccggccacgt
      241 caaccggccc ggcaactacg aagtcaagct cggcacgccg ttcgcgcagc tgctggagat
      301 ggcgggcggc gtacgcggcg gccgcaagct gaaggcggtg atccccggcg ggtcgtcgat
      361 gccggtgctg cctgccgcaa ccatgctggc caccgacatg gactacgact cgatcgccaa
      421 ggccggctcg atgctcggct cgggcgcggt gatcgtgatg gacgagacca cctgcatggt
      481 gcgcgcgctc gagcgcctgt cgtacttcta cttcgaggag tcctgcggcc agtgcacccc
      541 ctgccgcgaa ggcacgggat ggctgtatcg cgtggtgcac cgaatcgaga acgggcaggg
      601 gcgcagggag gacctcgcgc tgctcgacga cgtttcggac aacattgccg gccgcaccat
      661 ctgcgcgctg ggcgacgccg cggcgctgcc ggtgaagagc tttctgaagc acttccgcga
      721 cgagttcgag caccacgtcg agcaccagcg ctgcctggtg cagggcgccg agatcgagcc
      781 gaaatggggc cgtgccggcg cgcacgcgct gcccgccgtg aaggaagccg cctgatgccg
      841 aagctcgaaa tcgacggcag gccgatcgag gtggcgccgg gcaccccggt gatcgaggcg
      901 gcgaagcgcc tcggcatcta cgtgccgcac ttctgctacc actcgaagct ctcgattgcc
      961 gccaactgcc gcatgtgcct ggtcgaggtg gagaaagcgc ccaagcccat gcccgcctgc
     1021 gccaccccgg tgtccgacgg catgaaggtg tggacgcact cgccgagcgc gaagaaggcg
     1081 caaaacgggg tcatggagtt cctgctcatc aaccacccgc tcgactgccc gatctgcgac
     1141 cagggcggcg aatgccagtt gcaggacctg gcggtcggct acggcggctc ggcctcgcgc
     1201 taccaggagg aaaagcgggt cgtgctgcgc aaggacctgg ggcccctggt cgccgccgag
     1261 gaaatgacgc gctgcatcca gtgcacgcgc tgcgtgcgtt tcggcgagga gatcggcggc
     1321 gtcatggagc tcggcatggt gaaccgcggc gagctggccg agatcgtcgc gttcgtcggc
     1381 cgcggcgtgg actcggagct ctcgggcaac atgatcgacc tgtgcccggt gggcgcgctc
     1441 acctcgaagc cgttccgcta cgccgcgcgc acctgggagc tctcgcgccg gcgctcggtc
     1501 tcgccgcacg atggcctcgg ctcgaacctc gtggtgcagg tgaagcaaaa cgaggtgctg
     1561 cgcgtgctgc cgctcgagaa cgaagcggtg aacgagtgct ggctctcgga caaggaccgc
     1621 ttctcctacg aaggcctgaa cgccccggat cgactgacgc ggccgatggc gcgcatcgac
     1681 ggccgcctgc aggaagtcga gtgggaagtg gcgctcgagc gcgccgtggc gggcctcgcg
     1741 gacgccggcg tgctcgcctc gccgcacgcc acgctggagg agctgtacct cgccggcagg
     1801 ctcggtgcgg ccgatttccg gctgcgccac ggcgacttca gcgccgacgg gcggcgccag
     1861 ggcatcccct ggctcggcat gccgatcgcc gaactcgggt cgctggacgc cgtgctggtg
     1921 atcggctcgt tcctgcgcaa ggaccatccg ctccttgcgc accggctgcg gcaggccgcc
     1981 aagcgcggtg cgcagatcca cgtgctgcac tcggtcgacg acgactggct gatgccgatc
     2041 gcgagcaaga ccatcgtggc gccctccgca atcccgggcg ccatcgccgc tttcaaggac
     2101 gcactcgccg gcgcgaagaa cgccgccgtg ctgctcggca acttcgcgca gcagcatccg
     2161 caagcggcgg cgatccacgc cgcggcgcag gcgctgggcg tcaagctggg gttcttcggt
     2221 gaggcggcga actcggtcgg cggctacgtg gccgggctgc cgagcggcgg cggcctgccc
     2281 gaagccctga agaaggaggc gctgctgctg ctcaacgtcg agcccgcgct cgattgcgcc
     2341 gacccgcgcg cggccgcgcg cgcgaaattc atcgtcaatc tgaccgcctg gcggtccgag
     2401 ctcggcgacg tgctgctgcc gatcgcgccg ttcaccgaga cagcgggcac cttcatcaac
     2461 accgaaggcc gcgtgcaggg ctttcatgcg tgcgcgcggc cgctggggga ggcgcgtccg
     2521 gcatggaagg tgctgcgcgt cctcggcacg atgctcggca ggccgggatt cgaattcgac
     2581 acgatcgagc aggtgcgcga tgcctgcctc agcggccgcg acgtggccgc gctgctgggc
     2641 aacggcatcg acgccccggc ggggggggct gcgaccccgc cgaccggcat cgagcgcatc
     2701 gccgaggtgc cgatctactt cgccgatccg ctcgtgcgcc gcgcgtcgtc gctgcagaaa
     2761 acgcgcgatg cacgcgcgcc gcgcgcctgg atgaacgccc ggctgatgca aaggctcggc
     2821 ctcgccgacg gccagccggt gcgcgtgcgc cagggcgaag gcagcgcgaa gctcgctgcg
     2881 gcgctggacg accggctgcc tgacgactgc gtcaggatcg cggccgcgca cggcagcaca
     2941 gcgccgctcg gaccgatgtt cggcagcgtg caggtcgaga agctcagcgc ggaaaaggcg
     3001 gcctagatgg actggcgcct cgcgctcgtc gacacggcct ggaacctcgc gggcatcgtg
     3061 gccatcgtcg cgccgctgtt tctcgccgtg gcctacctga cgttgtggga gcgcaagctc
     3121 atcggctgga tccagatccg catcggcccc aaccgcgtcg gccctcgcgg cctgctgcag
     3181 ccgatcgcgg acggcgtgaa gctgctgctc aaggagatca tcgtcccgac caacgccagc
     3241 acggcgctgt tcatcgtggc gccgatcatg atgctgatgc cggcaatggc ggcgtgggcg
     3301 gtaatcccgt tcgcgcccga gctggtgctc gccgacatca acgccgggct gctctacgtg
     3361 ctggcgctga gctcgctcgg cgtctacggc gtgatcatcg ccggctgggc ctcgaactcc
     3421 aagtacgcgt tcctcggcgc catgcgctcg gcggcgcaga tggtgtccta cgagctcgcc
     3481 atgggcttcg cgctggtggt ggtgctgatg gtctcgggca gcctcaacct gtcggacatc
     3541 gtcgcgcagc aggggcgggg ccgcttcgcc gacatggggc tcacgtttct gtcctggaac
     3601 tggctgccgc tcctgccgat gctggtggtg tacttcgtcg ccggcgtggc cgagaccaac
     3661 cgcgcgccgt tcgacgtggt ggaaggcgag tcggagatcg tcgccggcca catgatcgag
     3721 tactcgggca tgagcttcgc gctgttcttt ctcgccgagt acatgaacat gctgctgatc
     3781 agcgcgctca cggcggtgat gttcttcggc ggctggcagc cgccgatctc gagcccggtg
     3841 ttcgacgcgg tgccgccgat tctctggctg ctcgccaaga tcttcgtcat cgtgtccatg
     3901 ttcctgtgga tccgcgccac gttcccgcgc taccgctacg accagatcat gcgcctgggg
     3961 tggaaggtgt tcattccgct gacgctggtg tggatcgcgg tgatcggcct gtggatgcag
     4021 acgccgtgga gcctgtggcg atgatcgcgc gcgtgaccga cttcttccgc gcgttcctgc
     4081 tgctcgagct gttcaagggg ctggcgctca ccgggcggca cctgttcgcg cgcaagatca
     4141 cggtgcagtt tcccgaggag aagacgccgc agagcccgcg cttccggggg ctacacgcgc
     4201 tgcgccgcta cccgaatggc gaagagcgct gcatcgcctg caagctgtgc gaggcggtat
     4261 gcccggcgct cgccatccac atcgagtc
//
LOCUS       DBNHKE010000169         1777 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1193516, whole genome shotgun sequence.
ACCESSION   DBNHKE010000169 DBNHKE010000000
VERSION     DBNHKE010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1193516"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..530
                     /locus_tag="AC16O9_11530"
     CDS             297..530
                     /locus_tag="AC16O9_11530"
                     /inference="COORDINATES: protein motif:HMM:NF016280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR6517283.1"
                     /translation="MIDKRVLEELGERLGEAARNSPAQDLEKNLRALLASFFDRFDVV
                     LRDDFDVQRKLLERAQAKLAALEARVAELESKR"
     gene            575..>1777
                     /locus_tag="AC16O9_11535"
     CDS             575..>1777
                     /locus_tag="AC16O9_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR6517284.1"
                     /translation="MALASVASRALAGIDAPEVTVEVHLGPGLPAFNIVGLPDTQVRE
                     AKDRVRAALNHAQFEFPARRITVNLAPADLPKDSSRFDLPIALGILAASGQLKGASLE
                     QHEFAGELSLTGELRPVRGALAMALGTARAGRAFVLPAENAAQAALAERAMVLPAASL
                     LEVCAHLAGEAIIAPYRNGAARTEPVYADFRDVKGQAAAKRALEVAATGGHSVLLLGP
                     PGTGKSMLAARFAGILAPMSPAEALESAAIQSAASAGFDPARWGARPLRAPHHTASRV
                     ALVGGGSPPRPGEISLAHHGVLFLDELPEFAREALEALREPLETGWISLARGTRQARF
                     PARFQLVAAMNPCPCGYLGHASGRCRCTPEAISRYRTRISGPLADRIDLKVEAPAPCD
                     AELVAAAGG"
BASE COUNT          257 a          630 c          628 g          262 t
ORIGIN      
        1 ccggacaccg agtgcggcga ggagccctgc gcgctcgcaa ggcccggcag ggccgcagcg
       61 gcggcaagtg cagcggcgag gatggtcttg cgcatgatgt cgatctccaa aagggttgcg
      121 gatggcaaac gcctatagcg cttagcatcc cccgtgccag agaagaatct cttttatttt
      181 catattgttg tcgccaacgt tggaaagggg cgcagcgata tagcgcacga gctgagtgca
      241 atggcgagcg ttccgcgcac ccgtttggtg cggagcgcag tgctacgctc gtggccatga
      301 tcgacaagcg tgttctggag gaactgggcg agcgcctcgg cgaggccgcg cgcaactcgc
      361 ccgcgcagga cctggagaag aacttgcgcg ccctgctcgc ttcgttcttc gaccggtttg
      421 acgtcgtcct gcgcgacgac ttcgacgtgc aaaggaagct cctcgaacgc gcgcaagcga
      481 aactcgcagc gctcgaggcg cgcgtcgccg agctcgaaag caagcggtaa accgcgcgcg
      541 gcgcggcgcg ttgtacccct gaaagggggt gccgatggcg ctggccagcg tggcgagccg
      601 cgcgcttgcg ggaattgacg cgcccgaggt caccgtcgag gtgcacctcg gacccgggct
      661 gccggcgttc aatatcgtcg ggcttcccga tacgcaggtg cgcgaagcca aggaccgcgt
      721 gcgtgccgcc ctgaaccacg cgcaattcga gttccccgcg cggcgcatca cggtcaatct
      781 cgcgcccgcg gacctgccga aggactcgag ccgcttcgac ctgccgattg cgctcggcat
      841 cctcgccgcg agcggccaat tgaaaggcgc cagcctcgag cagcatgaat tcgccggcga
      901 gctctcgttg accggcgagc tgcgcccggt gcgcggcgcg ctcgcgatgg cgctcggcac
      961 cgcgcgcgcc ggacgcgcct tcgtgctgcc cgcggagaac gccgcccagg ccgcgctcgc
     1021 cgagcgcgcc atggtgctgc ccgccgcgag cctgctcgag gtgtgcgcgc atctggccgg
     1081 cgaggcgatc attgcgccgt accggaacgg cgccgcgcgg accgagcccg tatatgccga
     1141 tttccgcgac gtgaagggac aggctgccgc caagcgcgca ctggaagtcg ccgccacggg
     1201 cggtcacagc gtgctactgc tcgggccccc gggcaccggc aagtcgatgc tggcggcgcg
     1261 cttcgccggc attctcgcgc ccatgagccc ggctgaggcg ctggagagcg ccgccatcca
     1321 gtcggccgcg agtgcgggct tcgacccggc gcgctggggc gcgcggccgc tgcgcgcgcc
     1381 gcaccacacc gcctcgcgcg tcgcgcttgt cgggggcggc agtccgcctc gtcccgggga
     1441 gatctctctt gcgcaccacg gggtgctgtt cctcgatgag ctgcccgaat tcgcgcgcga
     1501 ggcgctggag gcgctgcgcg agccgctgga gacgggctgg atctcgctcg cccgcggcac
     1561 gcgccaagcg cgctttcccg cgcgattcca gctggtggcg gcgatgaacc cctgcccctg
     1621 cggctacctc ggccatgcct cgggacgctg ccgctgcacg ccggaggcca tctcgcgcta
     1681 ccgcacgcgc atctccgggc cgctcgccga tcgcatcgac ctcaaggtcg aggcgccggc
     1741 gccctgcgac gccgagctgg tggccgcggc cggcggc
//
LOCUS       DBNHKE010000170        18891 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_400299, whole genome shotgun sequence.
ACCESSION   DBNHKE010000170 DBNHKE010000000
VERSION     DBNHKE010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 18891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_400299"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="AC16O9_11540"
     CDS             complement(<1..566)
                     /locus_tag="AC16O9_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6517285.1"
                     /translation="MRGWLLALWLAALPAWPQPAPVVVGAVVPETGHLAELARGMRQA
                     LLLWQDACNAAGGLLGRPVRLLLLDDRSEASAALVLYARLIEQERADLLIGPFGSAAS
                     VAAAAVAERARRVLVNATGVMPGVQRADWRYVFQVPAPVTEYGAGALALARAAGHARL
                     QVVARNDAVSREAAESLAAQAAASGMQAT"
     gene            complement(566..1303)
                     /locus_tag="AC16O9_11545"
     CDS             complement(566..1303)
                     /locus_tag="AC16O9_11545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR6517286.1"
                     /translation="MDPTTHDPARGALPFAAGAVARTIEEALLTRRSLRALRPDPVPR
                     ATVERILALASRAPSGTNIQPWKVYVVGGEARDRLVAAMHDEFMRHGEEAFKREFEYY
                     PTKWRDPYLARRRKIGWDLYGLLDIAKGEREKTQRQHARNYLFFDAPVGLAFTIERDM
                     PVGAWLDTGMFVQSVMLAARAFGLDTCPQAAIASAHAVLRRELPIPDAEIVVCGMSMG
                     YARADAAENTLVTEREPVSRFATFIGF"
     gene            1322..2671
                     /locus_tag="AC16O9_11550"
     CDS             1322..2671
                     /locus_tag="AC16O9_11550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6517287.1"
                     /translation="MDARLQSAFAELVGEKHVLTEPRDTVAYTTDWRKQYNAPAECVV
                     RPASTAEVAAVVALCARESLAIVPQGGNTGLCGGSVPSGTRREVVLSLSRLNRIRSID
                     ALNDTLTAEAGCVLAEVQRAADAAGRLFALSLAAEGSCQIGGNLSTNAGGVNVLRYGN
                     AREQVLGLEVVLPDGRVWDGMRALRKDNTGYDLKQLFLGAEGTLGVITAAVLKLHPKP
                     AAQASAWIGLASPAHAVALLAHLHAQIGERISAFELVSRRCLEAVLAYDRAARDPLPR
                     SHAWYVLAQFDQALEPEMLAGAPQVEDAVLASSTAQAQALWRIREFIPEAQFANVKHD
                     VSVPVSRVPDLIERADAALQAAFPGTPIYAFGHIGDGNIHYNVGDEALLARREEVNRL
                     VYGVVAALEGSISAEHGLGQLKRESIRRHKDALELELMRAVKRALDPQDLMNPGKVL"
     gene            complement(2668..3345)
                     /locus_tag="AC16O9_11555"
     CDS             complement(2668..3345)
                     /locus_tag="AC16O9_11555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6517288.1"
                     /translation="MLALAETRAEDFVLDLGSGDGRIVIHAARSYGARGLGVDLDPAL
                     VARARDDAARAGVGGLVRFEVQDVLRAELSRASVVTIYLVPYLMERLEPRLLDELRPG
                     ARVVSHAFALPSWPHDAAELVRLARPHPTQGDSSRIYLYVVPAQVRGRWRARGGWELR
                     VQQNFQRIELEALRDGRALAVSAASLRGTELAFSGAGFAFAGRVAGGSIAGELAGGTP
                     LVFERAP"
     gene            complement(3435..3692)
                     /locus_tag="AC16O9_11560"
     CDS             complement(3435..3692)
                     /locus_tag="AC16O9_11560"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR6517289.1"
                     /translation="MRLGAVLAVNLAEPEPRIRQFLQSMPLGFPVLLDAKRPATNAWK
                     VRQLPASFVVGPDGRIRYSHIGDLDWSSAAERRTLSELLPR"
     gene            complement(3689..4273)
                     /locus_tag="AC16O9_11565"
     CDS             complement(3689..4273)
                     /locus_tag="AC16O9_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6517290.1"
                     /translation="MTLKRRAVVQSLLADRGALLVIAGLGSTAWDCTAAGDDPLTFPM
                     WGAMGNAAMTGLGLALAQPARRVLVLTGDGEMLMGLGSLATIGVQRPANLSVVVIDNE
                     RYGETGMQATHTAHGVDLAAMARAAGFASAALVREAAGVSALRQAVHQRPGPHFAQIK
                     VAAEKEPLVLPPREGALLKNRFRQALLGEGAALQ"
     gene            complement(4270..4815)
                     /locus_tag="AC16O9_11570"
     CDS             complement(4270..4815)
                     /locus_tag="AC16O9_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HIR6517291.1"
                     /translation="MNAPLAASIDWSSATHEVLARLGFGVVGFVPDAGHKRLIELCQA
                     DARMRTVVLTTEEEGIGLATGAWLGGAKTALLMQSSGVGNIVNALGMVRECRVPLLLV
                     VTMRGEYGEFNPWQVPMGQATAAVLGAMGVVVQQANTAAEVATHVDAGARLAFGGYCA
                     AAVLISQRVIGIKSFQEQAAQ"
     gene            4904..5092
                     /locus_tag="AC16O9_11575"
     CDS             4904..5092
                     /locus_tag="AC16O9_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517292.1"
                     /translation="MANATWLEAALNGGWGRRYQLGIPMSVADIVAAGVACARDAILA
                     AGPCLAAPAEARVASNDR"
     gene            5089..5700
                     /locus_tag="AC16O9_11580"
     CDS             5089..5700
                     /locus_tag="AC16O9_11580"
                     /EC_number="4.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6517293.1"
                     /translation="MTDLAQFRAVLPRGDLWVFGYGSLMWSPGFPFAERSVGLLRGYH
                     RALCILSTRHRGTHRRPGLVMGLCRGGSCWGIAFRVPAARVPAALRKLWNREMPRRVY
                     QARLVPVRVVRGRKVRALAFVADPSHPQFVRELDLHSRAQLVAQGVGGRGACTEYVRS
                     TLGHMAELGVSDPHLARVLETAEAMRARASPRRARCVPKLSRT"
     gene            complement(5607..6758)
                     /locus_tag="AC16O9_11585"
     CDS             complement(5607..6758)
                     /locus_tag="AC16O9_11585"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR6517294.1"
                     /translation="MRFDFDAVVDRAGTWSSRWERYAGRDVIALWVADTDFRAPDCVL
                     EAMAARLRHGVLGYTAPPAELREAIVARMQRLYGWRIEPDWIVFLPGVVPGLHLAARH
                     LLRADEHALVPTPIYHHFKRAVELAPRAHADVPLVLQDGRWVLDEARLAAAVRPNTRL
                     LFLCNPQNPGGTIFTRAELERLAQFAERHGLAICSDEIHADLLLDAGKPHIPIAALAP
                     EVSRRTVTLASPNKTFNFPGAGCAWAIVEDPELRQAFSTDHHATVHDPSLFGYVAALA
                     AYRDGDAWLAAQLEYLRGNRDLVQRAVAQLAGLSMAHVEATYLAWIDARGLGVADPQA
                     HFLAHGVALSPGAQFGAPGYVRLNFGTQRARLGDALARIASAVSSTRAR"
     gene            complement(6826..8373)
                     /locus_tag="AC16O9_11590"
     CDS             complement(6826..8373)
                     /locus_tag="AC16O9_11590"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6517295.1"
                     /translation="MTGLALLGMVMLVVMIFVIFIGFPISFTLLFLAFTFGYLGFGHT
                     VVDLAYFQTIGMMKEELLAAVPLFVLMGFLTEQAGLMERLFVAFRNILAPLRGSLFAV
                     VILTAAVFAMATGIVGAAVTVLGIMASPIMVKTGYDGKLSAGAITAGGTLGILIPPSV
                     MLIVMGPVLGVSVADLYASAFGPGLMLACLYLGYLLGRAWLNPKLGPPVPKEERVHSM
                     ATMLKEVAIGVVPLLALITAALGSILAGLATPTEASAIGALGALLLTIAYGRLTYQGV
                     KRALLNTTATSSMVLFLAVTSNIFGAVFARMGTANWITETMLSLPLSPVMLLVVVLVL
                     IFLLGWPFEWPAIVLVFLPIFYPVMDALRPALAESLNIAPEMVMVWFGALVAVTLQTA
                     FLSPPVAMSAYYLKQVVKDWSLATIYKGMMEFMVLQVIAIAAIVAFPAIATWFPEKLQ
                     AESRAVKTEEVEDAATLEDYQPSGGYGEALREALEDMREEDALKNKEEEGAGDSLEKD
                     EATKAGK"
     gene            complement(8377..8928)
                     /locus_tag="AC16O9_11595"
     CDS             complement(8377..8928)
                     /locus_tag="AC16O9_11595"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6517296.1"
                     /translation="MAEQAPLHIRLVRMIDKFTDTTGTLVSWLNIPMMLCVSYEVAMR
                     YLFNAPTVWVFDVTYMLYGTIFMLGAAYALHKGAHIRTDFFFEKWSIRTKGVIDSIAS
                     IVFFFPGIFVFLLVSWNEGWYAYTINELSEQTPWRPVLWPFKMMVPLAALLLLIQGVS
                     ETIKSVWAARTGEELEHKEKYEI"
     gene            complement(9018..10124)
                     /locus_tag="AC16O9_11600"
     CDS             complement(9018..10124)
                     /locus_tag="AC16O9_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6517297.1"
                     /translation="MKVLVRSAVALAAATAFSAGAQAQTTLNMQATWPASLTLYENFL
                     YFADRISKVSGGQLKINTMPAGQVVPAFEVLDATHKKVLDGAHAWSGYWVGKNKTAIL
                     FTGGPGGTHGMDMIDALGWMHHGGGIDLANEFFQNELKLNVQWVPILPSGPQAFGWFK
                     RPIKNLADFKGMKCRQTGMAAEIWKTMGMTTVNMPGGEIIPAAQRGVIDCAEWVGGVE
                     DLRLGFQNVWKYHYTPGVHENVTIGEVLINGDVWKGLKPEHQELIKTVASETFIIWWA
                     KWQRQNADALKELQVKHGVRVLRTPPDILVEFLKTWDKIAKEEADKNPFFKKVLESQR
                     AYASVVVPAKRFMFPPYSFVANYYWPERAAGGKK"
     gene            complement(10213..10986)
                     /locus_tag="AC16O9_11605"
     CDS             complement(10213..10986)
                     /locus_tag="AC16O9_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR6517298.1"
                     /translation="MSYEIYAIKYGHHERKASENFLGGDPHDGPMPLDYFVWLIRGQG
                     REIVVDTGFNAAVAAKRGRRITLPVEEGLRLMQVDVAGVRDVVITHLHYDHVGNFDLF
                     PQATFHLQDLEMQYATGRCMTVDAMRHAYELEDVVGMVRRVYAGRVKFHDGDGELAPG
                     VTVHHVGGHTMGLQIVRVATRRGWVVLASDASHFYANMEQSRPFPIVYNVGDMVEGWH
                     RAQALAQSPAHVIPGHDPLVMARYPAPAAALEGKVVRLD"
     gene            complement(10983..12206)
                     /gene="tyrS"
                     /locus_tag="AC16O9_11610"
     CDS             complement(10983..12206)
                     /gene="tyrS"
                     /locus_tag="AC16O9_11610"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR6517299.1"
                     /translation="MTPVRQNDKPPVAQALELIKRGAEELIVEEELVRKLSRGTALRV
                     KLGLDPTAPDLHLGHTVVINKLRHFQELGHQVQFLIGDFTGMIGDPTGKNQTRPPLSR
                     EQILENAKSYREQAFKILDPDKTQILFNSEWSDKLGAEGMIRLAARYTVARILERDDF
                     AKRYRAGQPIAVHELLYPLMQGYDSVAMKADVELGGTDQKFNLLVGRELQKDYGQEPQ
                     CVLTTPLLEGLDGVNKMSKSLGNYVGIAEAPAEIFGKLMSISDELMWRYIELLSFEPL
                     ATVRKWRGEVEAGANPRDVKVRFAKEIVARFHSRAAAERAEEEFAQRFRHGQAPASMP
                     EVALHAPAGGLAVAQALKQAGLVPSVTEAVRNIEQGGVKLNGERLSDKARKLAAGETV
                     VAQVGKRKFARLRIS"
     gene            12256..13521
                     /locus_tag="AC16O9_11615"
     CDS             12256..13521
                     /locus_tag="AC16O9_11615"
                     /inference="COORDINATES: protein motif:HMM:NF039309.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6517300.1"
                     /translation="MNDYAPLKRTVLLVALAFSGAVVAFATVTPSPQAEATLLQRSVV
                     EPLALDAAEAILPAPTLYMAEERYQRGDTLQGLLARLGVADDDARQIARTPSMRFLRP
                     GYVVSAQLGERGELQRLSFLSARETEMVIERADGGFRAQEMPAPLRTEVEMRVGEIQS
                     SLFGAADAAGMPDAIALQVADVFAGDVDFHRELRRGDRFAAIYEQHYLGGRAVHTGRV
                     LAAEFVNQGRVLRAVYYSAGRGASGYYAPDGSNLRKAFLRSPLEYTRISSGFGMRRHP
                     FQRSWRAHSGIDYAAPAGTRVRAAGNGVIEFAGWKGGYGKAVILRHRGQYSTLYAHLS
                     RFAPGVRRGARVDQGDIIGHVGATGWATGPHLHYEFQIAGRARNPYAIAMPAGEPVPA
                     AHLPEFRLHAAPLVARLELLAAQPLARLE"
     gene            13518..14612
                     /locus_tag="AC16O9_11620"
     CDS             13518..14612
                     /locus_tag="AC16O9_11620"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR6517301.1"
                     /translation="MSAELYAGLMSGTSLDGVDAVLLDLAGPKPRLVRHAHRAFSPAL
                     RAELLALNAGAHGEIERAALCSNALARDYAAVVADLLAGAAVDRSALRAIGCHGQTVR
                     HRPEAGYTVQIGNAALLAELTQTRVVADFRSRDVAAGGQGAPLAPAFHAVMFSSGAED
                     RAVLNLGGMANLTWLPAAGAVAGFDTGPGNCLMDLWAQRHLGRPQDENGAWAAGARPD
                     AALLDVLLAEPYFALPPPKSTGRDLFNAAWLERRLERPLAPQVVQATLLELTAQSVAQ
                     ALRGRFPAARRLIACGGGVRNGALMARLEHLLSPVPVDSSAQHGIEPMQVEAAAFAWL
                     AQQALRGAPGNLPAVTGARGSRILGAVYPA"
     gene            complement(14662..15012)
                     /gene="erpA"
                     /locus_tag="AC16O9_11625"
     CDS             complement(14662..15012)
                     /gene="erpA"
                     /locus_tag="AC16O9_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR6517302.1"
                     /translation="MNAVTEMPAPLVFSDNAAGKVKQLIEEEGNAELKLRVFVSGGGC
                     SGFQYGFTFDEVQNEDDTVMQKNGVTLLIDPMSYQYLVGAEIDYQEGLEGAQFVIKNP
                     NATSTCGCGSSFSA"
     gene            complement(15052..15465)
                     /locus_tag="AC16O9_11630"
     CDS             complement(15052..15465)
                     /locus_tag="AC16O9_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6517303.1"
                     /translation="MFSKSPKPQSRIDSLIGATTRIEGNVFFSGGLRVDGAVRGNVAA
                     LPDQPGTLVVSEHARIDGEVAASHIVVNGTINGPVHAAETLELQVGSRVKGDVHYKAL
                     EIQQGAVVEGRLVHHGGAEIKGVELKLASGGDKPR"
     gene            complement(15480..16244)
                     /locus_tag="AC16O9_11635"
     CDS             complement(15480..16244)
                     /locus_tag="AC16O9_11635"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR6517304.1"
                     /translation="MAITTRFRKLRRRFGIAAPRVAVRSEVAWYLRWVGLILVLACSA
                     ALAAWMYDAGRKFAGFDRSEVEQELSAARRDLEATRAELERLRAVANAADSRLSIERT
                     AQLKLAQQIRGLEQETARLREELAIFESMLSAESRAANPLSINRFKVVPDVLPGEYRY
                     RLLLLLAPGTRRDRDFQGRLELVVGLTEGGKNAMMTFPEAGDPSASAFRLTFRHFHRV
                     EGIFRVDPKAKVESVQVRVYEAGSEQIKAAQTASPG"
     gene            complement(16329..16721)
                     /gene="rpsI"
                     /locus_tag="AC16O9_11640"
     CDS             complement(16329..16721)
                     /gene="rpsI"
                     /locus_tag="AC16O9_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR6517305.1"
                     /translation="MVGDYYYGTGRRKSSVARVFLKPGKGQIIVNGKPVDEFFGRETG
                     RMIVRQPLEVTKNVSTFDIKVNVFGGGENGQAGAVRHGISRALIEYDAGLKPALSAAG
                     LVTRDAREVERKKVGLHKARRRKQFSKR"
     gene            complement(16735..17163)
                     /gene="rplM"
                     /locus_tag="AC16O9_11645"
     CDS             complement(16735..17163)
                     /gene="rplM"
                     /locus_tag="AC16O9_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR6517306.1"
                     /translation="MKTFAAKPQDVVHGWYLVNAEGKVLGRLAARIASRLRGKHKPEF
                     TPHVDTGDYIVVVNAAKIRVTGRKMKDKKYYRHTGHPGGIRETNFAKLQVRHPGRALE
                     QAVKGMLPRGPLGYAMLKKLKVYGAATHPHSAQQPQTLEI"
     gene            17479..17898
                     /locus_tag="AC16O9_11650"
     CDS             17479..17898
                     /locus_tag="AC16O9_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6517307.1"
                     /translation="MECTIRWSGHGMSFIAETGSHHILAMDGAPDGGGRNLAPRPMET
                     VLAGAGGCTAYDVVVILKKSGQDVTGCEVRLEAERATTDPKVFTRIHTHFLVRGRNLK
                     RNVVEQAIRLSHEKYCSATAMLAKTAEITRDLEIVED"
     gene            complement(17895..18515)
                     /gene="coq7"
                     /locus_tag="AC16O9_11655"
     CDS             complement(17895..18515)
                     /gene="coq7"
                     /locus_tag="AC16O9_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR6517308.1"
                     /translation="MLDRGIVEFDRALRAVAGVHHAERPNPAAGMPEADLDAAERAHS
                     AALMRVNHVGEVCAQALYQGQALTARSAHTRASLERAAREEGDHLAWSMERIRALGGR
                     PSLLNPLWYAGALAMGIGAGLLGDRWNLAFLAETERQVEEHLTNHLDKLAPEDKPTRA
                     VVAAMREDEARHRGTAVGLGAADMPAPARRAMRTVARVMTTIAYRL"
     gene            complement(18536..>18891)
                     /locus_tag="AC16O9_11660"
     CDS             complement(18536..>18891)
                     /locus_tag="AC16O9_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517309.1"
                     /translation="PQLGLCRIWYADLPPEWQPPEMPCARAHSLAEKHGGRVIKAISP
                     GSFQDGRTLAMDYGPSQFGGLAPEQLPPPGYCRPWYDRVAADQQPAPMTCERAEQLVR
                     KNGGRVLYMPGLEHQ"
BASE COUNT         2864 a         6670 c         6437 g         2920 t
ORIGIN      
        1 gtcgcctgca tgccgctcgc cgccgcttgc gccgcgaggc tctcggcggc ttcgcgcgac
       61 acggcgtcgt tgcgcgcgac cacctgcagc cgcgcgtgcc cggcggcgcg cgccagggcg
      121 agcgcgcccg cgccatactc ggtcaccggc gccggcacct ggaacacgta gcgccagtcc
      181 gcgcgctgca ccccgggcat gactccggtg gcgttcacca gcacccggcg cgcgcgctcc
      241 gcgaccgcgg ccgcggccac ggacgccgcg ctgccgaagg ggccgatgag cagatcggcg
      301 cgctcctgct cgatcagccg cgcatagagc accagcgccg ccgatgcctc cgagcggtcg
      361 tccaggagca atagcctgac cggccgcccg aggaggcctc ccgcggcatt gcaggcgtcc
      421 tgccagagca ggagcgcctg gcgcatgccc cgcgcgagct cggcgagatg accggtctcg
      481 gggaccacgg ccccgaccac caccggcgcc ggctgcggcc acgccggcaa cgccgccagc
      541 cacagcgcga gcagccagcc gcgcatcaga agccgatgaa tgtcgcgaag cgcgacaccg
      601 gctcgcgctc ggtgaccagc gtgttctccg ccgcgtcggc gcgtgcgtag cccatcgaca
      661 tcccgcacac gacgatctcg gcgtccggga tcggcagctc gcgccgcagc accgcgtgcg
      721 ccgaagcgat tgcggcctgc gggcaggtgt ccagcccgaa tgcgcgcgcc gcgagcatca
      781 cgctctgcac gaacatgccg gtatccagcc aggcgccgac cggcatgtca cgctcgatgg
      841 tgaaggccag gccgaccggc gcgtcgaaga acaggtaatt gcgcgcatgc tggcgctgcg
      901 tcttctcgcg ctcgcccttg gcgatgtcga gcagcccgta caggtcccag ccgatcttgc
      961 ggcgccgcgc caggtaggga tcgcgccact tggtggggta gtactcgaac tcccgcttga
     1021 aggcttcctc gccatggcgc atgaactcgt cgtgcatcgc cgcgacgagc cgatcgcgcg
     1081 cctcgccgcc gacgacgtac accttccagg gctggatatt ggtgccggag ggcgcgcggc
     1141 tggccagcgc gaggatgcgc tccaccgtcg cgcgcggcac cgggtcgggc ctcaaagcgc
     1201 gcaggctgcg ccgcgtgagc agcgcctcct cgatcgtgcg cgcgacagcg ccggcggcaa
     1261 agggcagcgc gccccgcgcc gggtcgtggg tcgtcggatc catcgggcga taatacccaa
     1321 catggatgcc cgcctgcagt ccgcgttcgc tgagctggtc ggcgaaaagc acgtgctcac
     1381 cgagccgcgc gacaccgttg cgtacacgac cgactggcgc aagcagtaca acgcgcccgc
     1441 cgaatgcgtc gtgcggccgg caagcacggc ggaagtcgct gcggtggtcg cgctgtgcgc
     1501 gcgcgaaagc ctcgcaatcg tcccccaggg tggcaatacg ggcctttgcg gcggctcggt
     1561 gccgagcggg acgcgccgcg aggtggtgct gtcgctttcg cgcctgaacc gcattcgcag
     1621 catcgacgcg ctgaacgaca ccctgaccgc cgaggccggc tgcgtgctgg ccgaagtgca
     1681 gcgcgcggcc gatgccgcgg ggcggctctt tgcgctgtcg ctcgccgccg agggcagctg
     1741 ccagatcggg ggcaacctct ccaccaacgc cggcggcgtc aacgtgctgc gctacggcaa
     1801 cgctcgcgag caggtgctgg gcctcgaggt ggtgctgccc gacggcaggg tgtgggacgg
     1861 gatgcgcgcg ctgcgcaagg acaataccgg ctatgacctg aagcagctgt ttctcggcgc
     1921 ggaaggcaca ctgggcgtga tcaccgccgc cgtgctgaag ctgcatccga agcccgcggc
     1981 gcaggcaagc gcctggatcg ggctcgccag cccggcgcac gccgtggcgt tgctggcgca
     2041 tctgcatgcg cagatcggcg agcgcatcag cgccttcgag ctcgtgtcgc gccgctgcct
     2101 ggaggcggtg ctggcgtacg accgcgcggc gcgcgacccc ctgccgcgct cgcacgcctg
     2161 gtacgtcctc gcccagttcg accaggcgct cgagcccgaa atgctggccg gcgcgccgca
     2221 ggtcgaggac gcggtgctcg cctcgagcac ggcgcaggcg caggcgctgt ggcgcatccg
     2281 ggaattcatc ccggaagccc agttcgccaa cgtcaagcac gacgtgtcgg tgcccgtctc
     2341 gcgcgttccc gacctcatcg agcgggccga cgccgcgctg caggcggcgt ttcccggcac
     2401 accgatctac gcttttggcc acatcggcga cggcaatatc cactacaacg tgggcgacga
     2461 ggcactgctc gcgcgccgcg aggaggtaaa ccgcctggtc tacggcgtag tcgccgcgct
     2521 ggagggctcg atctccgcag agcacggcct cgggcagctc aagcgcgagt cgatccggcg
     2581 ccacaaggac gcgctggagc tggaactgat gcgcgcagtg aagcgtgccc tcgacccgca
     2641 ggacctgatg aaccccggga aggtgctcta gggcgcgcgc tcgaagacga gcggcgtgcc
     2701 gcccgcaagc tcgcccgcga tactgccgcc cgcgacgcgg cccgcgaagg cgaagcccgc
     2761 accggagaaa gcgagctcgg tgccgcgcaa gctcgccgcg ctcaccgcga gtgcgcgccc
     2821 atcgcgcagc gcctcgagct cgattctctg gaaattctgc tgcacgcgca gctcccagcc
     2881 accgcgcgcg cgccaccggc cgcgcacctg cgccggcacg acgtacagat agatgcggct
     2941 cgaatcgccc tgcgtcgggt gcgggcgcgc gagccgcacc agctcggccg cgtcatgcgg
     3001 ccagctcggc agtgcgaacg catgcgacac gacgcgcgcg ccgggccgca gttcgtcgag
     3061 caggcgcggc tcgagccgct ccatcaggta cggcaccagg tagatcgtga ccacgctcgc
     3121 ccgcgacagc tcggcgcgca gaacgtcctg cacctcgaag cgcaccagtc cgccgacgcc
     3181 cgcgcgcgcc gcgtcgtccc gcgcgcgcgc caccagggcc ggatcgaggt ccacgcccaa
     3241 gccgcgcgcg ccataggacc gcgccgcatg gatgacaatg cgtccgtcgc ccgagccgag
     3301 gtcgaggacg aagtcctcgg cgcgcgtttc agcgagcgcc agcatgcgcg ccacgacctc
     3361 gggcggggtg gtgaagaacg gcgcccgtac cggcgcctcc tgcgcggcgg catgcgccgc
     3421 cagcagcagg cccgctagcg cggcagcagt tcggagaggg tccggcgctc ggccgcgctc
     3481 gaccaatcga gatccccgat gtggctatag cggatgcggc cgtcgggccc gacgacgaag
     3541 ctcgccggca actggcgcac cttccaggcg ttcgtcgcgg gcctcttggc atcgagcaga
     3601 acgggaaagc cgagcggcat cgactgcagg aactggcgga tgcgcggctc gggctcggcc
     3661 aggttcaccg ccagcacggc gccaagcctc attgcagggc ggcgccctcg cccagcagcg
     3721 cctggcggaa gcggttcttc agcagcgcgc cctcgcgcgg cggcagtacc agcggctcct
     3781 tttccgccgc caccttgatc tgggcgaagt gcggacccgg cctctggtgc acggcctggc
     3841 gcagggccga gacgcccgcg gcctcgcgta ccagagcggc cgaggcgaac ccggccgcgc
     3901 gcgccatggc ggcgaggtcg acgccgtgcg ccgtgtgggt ggcctgcatc ccggtttcgc
     3961 cgtagcgctc gttgtcgatc accaccaccg agagattcgc gggcctttgc acgccgatgg
     4021 tggcgagcga gccgaggccc atcagcatct cgccgtcccc ggtgagcacc agcacgcgcc
     4081 gcgccggctg cgcgagcgcg aggccgaggc ccgtcatcgc cgcgttgccc atcgcgcccc
     4141 acatcgggaa ggtgagtgga tcatcgcccg ccgcggtgca gtcccaggcg gtcgagccca
     4201 ggccggcgat gaccagcagc gcgccgcgat cggcgagcag cgattgcacg acggcgcgcc
     4261 gcttgagcgt cattgcgccg cctgctcctg gaagctcttg atgccgatca cgcgctggga
     4321 tatgagcacc gcggccgcgc aatagccgcc gaaagcgagc cgtgcgcccg catcgacgtg
     4381 cgtggcgacc tcggccgccg tattcgcctg ctgcacgacg acgcccatcg cccccagcac
     4441 cgccgcggtc gcctgcccca tcggcacttg ccaggggttg aactcgccat actcgccgcg
     4501 catggtcacg acgagcagca gcgggacgcg gcactcgcgc accatgccca gcgcattcac
     4561 gatattgccg acgccgctcg attgcatgag cagcgcagtc ttcgcgccgc cgagccatgc
     4621 gccggtcgca agcccgatgc cctcttcctc ggtggtgagg accaccgtgc gcatgcgcgc
     4681 gtcggcctgg cacagctcga tcaggcgctt gtggcccgcg tcgggcacaa agccgaccac
     4741 gccgaagccc aggcgcgcga gcacctcgtg cgtggcggag gaccaatcga tgctggcggc
     4801 gagtggggcg ttcaaggcgt gcgattctaa accacccgta cggcgcggtg cggttcgtca
     4861 cggttttcct gccgccgctt ctataatgtc cgcaccatcg cccatggcga acgcgacctg
     4921 gctcgaagcg gctctgaacg gtggctgggg acgccgttac cagctcggca tccccatgtc
     4981 ggtggccgac atcgtcgccg caggcgtcgc ctgcgcgcgc gacgcgattc tcgcagcggg
     5041 cccatgcctc gcggcgccgg ccgaggcgcg cgtggcgtcg aacgaccgtt gaccgacctc
     5101 gcgcaatttc gcgccgtgct gccgcgcggc gacctgtggg tgttcggcta cggctcgctg
     5161 atgtggtcgc cggggttccc cttcgccgag cgctccgtgg ggctgctgcg cggctaccat
     5221 cgcgcgctgt gcatcctgtc gacacgccac cgcggcacgc atcgccgccc ggggctggtc
     5281 atgggactgt gccgcggcgg ttcctgctgg ggcatcgcgt tccgcgttcc cgcggcgcgc
     5341 gtgcccgccg cgctgaggaa gctttggaac cgcgagatgc cgcgccgcgt gtaccaggcg
     5401 cgcctcgtgc cggtgcgcgt tgtgcgtggc cgcaaggtgc gtgcgctcgc cttcgtcgcc
     5461 gatccgtcgc atccgcagtt cgtgcgcgag ctcgacctgc atagccgcgc gcagctcgtc
     5521 gcgcagggcg tgggcgggcg cggcgcgtgc accgagtacg tacgctcgac gctcggccac
     5581 atggccgagc tcggcgtgtc cgaccctcac ctcgcgcgcg tgctggagac cgccgaggcg
     5641 atgcgcgcga gggcgtcacc gagacgcgcg cgctgcgtgc cgaagttgag ccggacgtag
     5701 cctggcgcgc cgaactgcgc accgggcgaa agcgccacgc cgtgcgcgag gaagtgcgcc
     5761 tgcgggtccg ccacgccgag gccgcgtgcg tcgatccagg cgaggtaggt cgcttcgacg
     5821 tgcgccatcg acaagcccgc caactgggcc acggcacgct gcacgaggtc gcgattgccg
     5881 cgcaggtact cgagctgcgc ggcgagccag gcgtcgccat cgcggtacgc ggccagcgcc
     5941 gccacgtagc cgaacagcga cggatcgtgc acggtggcgt ggtgatcggt cgagaacgcc
     6001 tggcgcaact ccgggtcctc gacgatcgcc caggcgcagc ctgcgcccgg aaagttgaag
     6061 gtcttgttcg gcgaggcgag cgtcacggtg cggcgcgaga cttcgggtgc gagcgcggcg
     6121 atggggatat gcggcttgcc cgcgtcgagc agcagatcgg cgtggatctc gtccgagcag
     6181 atggccaggc cgtgccgctc ggcaaactgc gccaggcgct cgagctcggc acgggtgaag
     6241 atggtgccgc ccgggttctg cgggttgcac agaaacagca ggcgcgtgtt cgggcgcact
     6301 gcggcagcca ggcgcgcctc gtccagcacc cagcgcccgt cctgcagcac gagcggcacg
     6361 tccgcgtgcg cgcgcggcgc gagctccacg gcgcgcttga aatggtggta gatgggcgtc
     6421 ggcaccagcg cgtgctcgtc ggcgcgcagc aggtggcgcg cggccaggtg caaccccgga
     6481 accacgcccg gcaggaacac gatccagtcg ggctcgatgc gccagccgta caggcgctgc
     6541 atgcgcgcga caatcgcctc gcgcagctcg gccggcggcg ccgtgtagcc gagcacgccg
     6601 tggcgcagcc gcgccgccat cgcctccagc acgcagtccg gagcgcggaa gtcggtgtcc
     6661 gccacccaga gcgcgatgac gtcgcgtccg gcgtagcgct cccagcgcga ggaccaggtc
     6721 ccggcgcgat ccaccacggc atcgaaatcg aatcgcatgc gcagcttgta gcacaaaaaa
     6781 aaacgccggg cgcgtggccc ggcgctgccg tctaggcgct taccgctact tgccagcctt
     6841 ggtcgcttcg tctttttcca ggctgtcccc cgcgccctcc tcctccttgt tcttcagcgc
     6901 gtcctcttcg cgcatgtcct cgagcgcctc gcgcagcgcc tcgccgtagc cgcccgaggg
     6961 ctggtagtcc tccagcgtgg ccgcgtcctc cacttcctcg gtcttgacgg cgcgcgactc
     7021 cgcctgcagc ttctcgggga accaggtggc gatcgccggg aacgccacga tcgcggcgat
     7081 ggcgatcacc tgcaggacca tgaactccat cattcccttg tagatcgtgg cgagcgacca
     7141 gtccttcacc acctgcttca ggtagtacgc cgacatcgcc acgggcggcg acaggaacgc
     7201 cgtctgcagc gtcaccgcca ccagcgcccc gaaccacacc atgaccatct cgggcgcgat
     7261 gttgagggac tcggcgagcg cgggccggag cgcgtccatc accgggtaga agatcggcag
     7321 gaacaccagc acgatcgccg gccactcgaa cggccagccg agcaggaaga tcagcaccag
     7381 caccaccacc agcagcatca ccggcgacag cggcagcgag agcatggttt cggtaatcca
     7441 gttcgcggtg cccatgcgcg cgaacaccgc gccgaagatg ttcgacgtca ccgccagaaa
     7501 cagcaccatg ctggaggtcg cggtggtgtt gaggagcgcg cgcttgacgc cctggtaggt
     7561 aagccggccg taggcgatcg tgagcagcaa ggcgcccagc gcgccgatcg cggaggcctc
     7621 ggtgggcgtg gcgagaccgg ccaggatcga gccgagcgcg gcggtgatca gggcgagcag
     7681 cggcaccacg ccgatggcga cttccttcaa catggtcgcc atgctgtgca cgcgctcttc
     7741 cttcggcacc ggcggcccga gcttcggatt cagccaggca cggcccagca ggtagccgag
     7801 gtaaaggcag gccagcatga gcccggggcc gaacgccgag gcgtagaggt cggccaccga
     7861 gacgccgagc accggcccca tgacgatcag catcaccgag ggcgggatga ggatgccgag
     7921 cgtgccgccg gcggtgatcg cgcccgccga cagcttgccg tcgtatccgg tcttcaccat
     7981 gatcgggctc gccatgatgc cgagcaccgt cactgcggcg ccgacgatgc cggtcgccat
     8041 ggcgaacacc gccgccgtca ggatgaccac cgcgaacagc gagccgcgca gcggcgcgag
     8101 gatgttgcgg aaggcgacga acagccgctc catcagtccc gcctgctcgg tcaggaaccc
     8161 catcagcacg aacagcggca ccgcggcgag cagctcctcc ttcatcatgc cgatggtctg
     8221 gaaataggcc aggtcgacca ccgtgtggcc gaacccgagg tagccgaaag tgaacgcgag
     8281 gaacagcagc gtgaaactga tcggaaagcc gatgaagatg acgaagatca tcaccaccag
     8341 catgaccatc ccgagcaggg cgaggccggt catgcgctag atctcgtact tttccttgtg
     8401 ctcgagctct tcgcccgtgc gcgccgccca gacgctcttg atcgtctccg agacgccctg
     8461 gatgaggagc agcagcgcgg cgagcggcac catcatcttg aacggccaca gcaccgggcg
     8521 ccacggcgtc tgctccgaga gctcgttgat cgtgtaggcg taccagccct cgttccagct
     8581 gacgaggagg aacacgaaga tgccgggaaa gaagaacacg atggaggcga tggagtcgat
     8641 cacgcccttg gtgcggatcg accacttctc gaagaagaag tcggtgcgga tgtgcgcccc
     8701 cttgtgcagt gcgtaggccg cgcccagcat gaagatcgtg ccgtagagca tgtaggtgac
     8761 gtcgaacacc cagaccgtgg gcgcgttgaa caggtaccgc atcgccactt cgtagctcac
     8821 gcacagcatc atggggatgt tgagccacga caccagcgtg cctgtggtat cggtgaactt
     8881 gtcgatcatc cgcacgaggc ggatatgcag cggggcctgc tcagccatgc tcaacctcga
     8941 ctggcgccgg cacttggttc aagggacgcg gaaagaaaac gggggcggtg ttccgccccc
     9001 gtcaagggcc tttgcggcta cttcttgccg cccgcggcgc gctcgggcca atagtagttg
     9061 gcgacgaacg agtacggcgg gaacatgaag cgcttggccg ggaccaccac cgacgcatag
     9121 gcgcgctgcg attcaagcac cttcttgaag aacgggttct tgtccgcttc ctccttggcg
     9181 atcttgtccc aggtcttcag gaactcgacc aggatgtcgg ggggggtgcg caggacgcgc
     9241 acgccgtgct tcacctgcag ctccttcagg gcatcggcgt tctggcgctg ccacttcgcc
     9301 caccagatga tgaacgtctc gctggcgacc gtcttgatca gctcctggtg ctcgggcttg
     9361 agacccttcc acacgtcgcc gttgatcagc acctcgccga tcgtcacgtt ctcgtgcacg
     9421 cccggggtgt agtggtactt ccagacgttc tggaagccga ggcgcaggtc ctccacgccg
     9481 cccacccact cggcgcagtc gatcacgccg cgctgcgcag ccgggatgat ctcgccgccc
     9541 ggcatgttga cggtggtcat gcccatcgtc ttccagatct cggcagccat gccggtctgg
     9601 cggcacttca tgcccttgaa gtcggccagg ttcttgatcg gccgcttgaa ccagccgaag
     9661 gcctgcgggc ccgacggcag gatcggcacc cattgcacgt tgagcttcag ctcgttctgg
     9721 aagaactcgt tggcgagatc gatgccgccg ccatggtgca tccagccgag cgcgtcgatc
     9781 atgtccatgc cgtgcgtgcc gcccgggccg ccggtgaaca ggatcgcggt cttgttcttg
     9841 ccgacccagt agcccgacca ggcatgcgcg ccgtccagca ccttcttgtg cgtggcgtcg
     9901 agcacctcga aggccggcac cacctggccg gcgggcatgg tgttgatctt gagctggccg
     9961 ccgctcactt tcgaaatgcg gtcggcgaag tacaggaagt tctcgtacag cgtcaggctt
    10021 gcaggccagg tggcctgcat gttgagcgtc gtctgggctt gcgcgcccgc ggaaaaagcg
    10081 gttgcagccg ccagcgcgac ggcggaacgg accagtacct tcatggtttc ctcccctgtg
    10141 gatgacgatg cgtgatcgca gcgtacacgc cggatttttc ccggtcaata gagggataag
    10201 cgggaaacgg cgtcaatcca gccgcacgac cttgccctcc agcgcagccg ccggcgccgg
    10261 gtagcgcgcc atcaccagcg gatcatgtcc gggtatgacg tgtgcaggcg attgcgcgag
    10321 cgcctgcgcc cgatgccagc cctcgaccat gtcgcccacg ttgtagacga tcggaaaggg
    10381 ccggctttgc tccatgttgg cgtagaaatg gctcgcgtcg gaggcgagca ccacccagcc
    10441 gcggcgcgtg gcgacacgca cgatttgcag gcccatggtg tggccgccga cgtggtgcac
    10501 ggtgaccccc ggcgcgagct cgccgtcgcc gtcgtggaat ttcacgcgcc cggcgtacac
    10561 gcggcgcacc atgccgacca cgtcctcgag ctcataggcg tggcgcattg cgtccacggt
    10621 catgcagcgg ccggtcgcgt actgcatctc caggtcctgc aggtggaagg tcgcctgcgg
    10681 gaacaggtcg aagttgccca cgtggtcgta atgcaggtgc gtgatcacca cgtccctcac
    10741 gccggccacg tcgacctgca tgaggcgcag gccttcctcg acgggcagcg tgatgcggcg
    10801 cccgcgcttt gccgccaccg cggcattgaa gcccgtgtcg acgacgatct cgcgcccctg
    10861 gccgcggatc agccagacga agtagtcgag cggcatcggc ccgtcgtgcg ggtcgccgcc
    10921 gagaaagttc tcgctggcct tgcgctcgtg gtgcccgtac ttgatggcgt agatttcgta
    10981 gctcatgaga tcctcagccg cgcgaacttg cgcttgccga cctgggcgac cacggtctcc
    11041 ccggccgcaa gcttgcgcgc cttgtccgag agccgctcgc cgttcagctt caccccgccc
    11101 tgctcgatgt tgcgcaccgc ctcggtgacg ctgggcacga ggccggcctg cttgagcgcc
    11161 tgcgccaccg cgaggccgcc ggcgggcgcg tgcagcgcca cttccggcat cgacgcgggc
    11221 gcctgcccgt gccgaaagcg ctgcgcgaac tcctcctcgg cacgctcggc cgcggcacgc
    11281 gaatggaaac gcgccacgat ctcctttgcg aagcgcacct tgacgtcgcg cggattggcg
    11341 cccgcctcga cctcgccgcg ccacttgcgc accgtcgcga gcggctcgaa cgacagcagc
    11401 tcgatgtagc gccacatcag ctcgtcggag atcgacatca gcttgccgaa gatctcggcg
    11461 ggcgcctcgg cgatgccgac atagttgccg agcgacttgg acatcttgtt gacgccgtcc
    11521 aggccctcga gcagcggcgt ggtgagcacg cactgcggct cctggccgta gtccttctgc
    11581 agttcgcgcc ccacgagcag gttgaacttc tggtcggtgc cgccgagctc gacgtcggcc
    11641 ttcatggcca ccgagtcgta gccctgcatc agcggataga gcagctcgtg caccgcgatc
    11701 ggctgcccgg cccggtagcg cttggcgaag tcgtcgcgct cgaggatgcg cgccacggtg
    11761 tagcgcgcgg cgagccggat catgccctcg gcgccgagct tgtcggacca ttccgagttg
    11821 aacaggatct gcgtcttgtc ggggtcgagg atcttgaacg cctgctcgcg gtagctcttc
    11881 gcgttctcca ggatctgctc gcgcgacagc ggcgggcgcg tctggttctt gcccgtcggg
    11941 tcgccgatca tgccggtgaa atcgccgatc aggaactgca cctggtgccc cagctcctgg
    12001 aaatggcgca gcttgttgat gaccaccgtg tggccgaggt gcaggtcggg cgcggtcggg
    12061 tccaggccga gcttcacgcg cagcgccgtg ccgcgcgaga gcttgcgcac gagctcttcc
    12121 tcgacgatca gctcctcggc gccgcgcttg atcagctcga gggcctgcgc caccgggggc
    12181 ttgtcgtttt gccgcactgg ggttatttgc tagactgatg caggcatctg aatagaaaag
    12241 aattctagcc gatccgtgaa cgattacgcg ccgctcaagc gcactgtcct gctggtggcc
    12301 ctcgcgttct cgggcgccgt ggtcgccttc gccacggtaa cgccctcgcc gcaggcggaa
    12361 gccacgctgc tgcaaaggtc ggtggtcgag ccgctcgcgc tggatgcggc cgaggcgatc
    12421 ctgccggcgc cgacgctcta catggcggaa gagcgctatc agcgcggcga cacgctgcaa
    12481 gggctcctgg cgcgactcgg ggtcgccgac gacgacgcgc gccagatcgc ccgcaccccc
    12541 tcgatgcgct tcctgcgccc gggatacgtc gtctccgcgc aactcggcga gcgcggcgag
    12601 ctgcagcgcc tgagcttcct ctcggcgcgc gagaccgaaa tggtgatcga gcgcgcggac
    12661 ggcggattcc gcgcccagga gatgcccgcg cccttgcgca ccgaggtgga aatgcgcgtc
    12721 ggggagatcc agagctcgct ctttggcgcg gcagacgcgg ccggcatgcc cgacgccatc
    12781 gccctgcagg tcgccgacgt ctttgccggc gacgtggact ttcaccgcga gctgcgccgc
    12841 ggcgaccgct ttgccgcgat ctacgagcag cattatctgg gcggtcgcgc agtgcacacc
    12901 ggccgcgtgc tcgcggcgga gttcgtcaat caagggcgcg tgctgcgcgc cgtgtactac
    12961 tcggcggggc gcggggcgag cggctactac gcgcccgacg gcagcaatct gcgcaaggca
    13021 ttcctgcgct cgccgctcga gtacacgcgc atcagctccg gtttcggcat gcgccggcac
    13081 ccgttccagc gctcctggcg ggcgcattcg ggcatcgatt acgccgcgcc cgcgggcacg
    13141 cgcgtgcgcg ccgccggcaa cggcgtcatc gagttcgcgg gctggaaggg cggctacggc
    13201 aaggcggtga tcctgcgcca ccgcggccag tactcgacgc tgtacgcgca cttgtcgcgc
    13261 ttcgcccccg gggtgcggcg cggcgcgcgc gtcgaccagg gcgacatcat cggccatgtg
    13321 ggcgcgacgg gctgggccac cgggccgcac ctgcactacg agttccagat cgcggggcgg
    13381 gcgcgcaatc cctacgccat tgccatgccg gcgggcgagc cggtgcccgc agcgcatctg
    13441 cccgagttcc ggctgcacgc ggcgccgctg gttgcgcgcc tcgagctgct tgccgcgcag
    13501 ccgctcgcgc ggctggagtg agcgccgagc tctacgcggg cctgatgtcg gggaccagcc
    13561 tcgacggggt cgacgcggtg ctgctcgacc tcgcgggtcc caagcccagg ctcgtgcgcc
    13621 acgcgcaccg ggctttctcg ccggcgctgc gggccgagct gctggcgctc aatgcgggcg
    13681 cgcacggcga aatcgagcgt gccgccctct gttccaacgc gctggcgcgc gactacgctg
    13741 ccgtcgtcgc cgacctcctt gcaggcgctg cggtggatcg cagcgcgctg cgcgccatcg
    13801 gctgccacgg ccagaccgtg cgccatcggc ccgaggccgg ctacacggtg cagatcggca
    13861 acgccgcgct gctcgccgag ctcacgcaga cccgcgtcgt cgccgacttt cgcagccgcg
    13921 acgtggccgc cggcggccag ggcgcgccgc tggcgcccgc atttcatgcc gtcatgttct
    13981 cgagcggcgc cgaggaccgc gcggtcctga acctgggcgg catggccaac ctgacctggc
    14041 tgccggccgc gggcgcggtg gcgggcttcg acaccggccc cggaaactgc ctgatggatc
    14101 tgtgggcgca gcggcacctg ggccggccgc aggacgaaaa cggggcctgg gcggcgggcg
    14161 cgcggccgga cgccgcgttg ctcgatgtgc tgctcgcgga gccgtacttc gcgctgccgc
    14221 cgcccaagag caccgggcgg gacctgttca acgcggcgtg gctcgagcgc cggctcgagc
    14281 gcccgctcgc cccccaggtc gtgcaggcca cgctcctcga gctgacggcg caaagcgtcg
    14341 cgcaggcgct gcgcggccgc ttcccggccg cgcgccgctt gatcgcctgc ggcggcggcg
    14401 tgcgcaacgg cgcactgatg gcgcggctcg agcacctgct gtcgcccgtg ccggtggaca
    14461 gcagcgcaca gcacggcatc gagccgatgc aggttgaagc cgcggccttt gcctggctcg
    14521 cgcagcaggc gctgcgggga gcgccgggaa atctgcccgc ggtcaccggc gcgcgcgggt
    14581 cccgcatcct cggcgccgtc tatccggcct agaaaacgca aagggggccg cagccccctg
    14641 tcgaggaatc gacccgagcc ctcaggccga gaacgaagag ccgcagccgc aggtgctggt
    14701 ggcgttcggg ttcttgatca cgaactgcgc gccttccagg ccttcctggt agtcgatctc
    14761 ggcacccacc agatactggt agctcatcgg gtcgatgagc agcgtgacgc cgttcttctg
    14821 catcacggtg tcatcttcgt tctgcacttc gtcgaaagtg aagccgtact ggaatccgga
    14881 gcagccgccg cccgacacga acacgcgcag cttgagctcc gcgttgcctt cttcctcgat
    14941 cagctgcttt accttgcccg cggcgttgtc ggagaacacc aggggtgccg gcatttcggt
    15001 gactgcattc attgcgcgct ccaatgtgcc aaactgcgtt gcgatcggtc gttagcgcgg
    15061 cttgtcgccg ccggaggcga gcttgagctc gacccccttg atctcggcgc cgccatggtg
    15121 cacgagccgc ccttccacca cggcaccctg ctgtatctcg agtgccttgt aatgcacatc
    15181 ccctttgacg cgcgagccga cctggagttc aagcgtctcg gccgcgtgca ccgggccgtt
    15241 gatggtgccg ttcaccacga tgtgcgaagc ggcgacttcg ccgtcgatgc gcgcatgctc
    15301 ggagaccacc agcgttcccg gctggtcggg cagcgccgcg acgttgccgc gcacggcgcc
    15361 gtcgacgcgc aggccgccgc tgaagaacac gttgccctcg atgcgcgtgg tggcgccgat
    15421 caggctgtcg atgcggcttt gcggcttcgg actcttgctg aacatgtctg ctccttgcct
    15481 catcccggcg acgcggtctg cgccgccttg atctgttccg accccgcctc gtacacccgc
    15541 acctgaacgc tctccacttt ggctttcgga tccacgcgga agatgccttc gacgcggtgg
    15601 aagtgcctga aagtcaagcg gaaagccgag gcgctggggt ctcccgcttc agggaaagtc
    15661 atcatagcat ttttgccccc ctccgtcagg ccgaccacca gctcgagccg gccctggaag
    15721 tcgcggtcgc ggcgtgtgcc gggcgcaagc agcagcagca gccggtagcg gtactcgccg
    15781 ggcagcacgt cgggcaccac cttgaagcgg ttgatcgaca gcggattggc ggcacgcgac
    15841 tccgccgaga gcatgctctc gaagatcgcc agctcctcgc gcaggcgcgc ggtctcctgc
    15901 tccaggccgc ggatctgctg cgcgagcttc aactgcgcgg tgcgctcgat cgacagccgg
    15961 ctgtcggcgg cattggcgac ggcgcgcagg cgctcgagct cggcgcgcgt ggcttccagg
    16021 tcgcggcgcg cggcgctgag ctcctgctcc acctcgctgc ggtcgaaccc ggcaaacttg
    16081 cggccggcgt cgtacatcca ggcggcgagc gcggccgagc acgcgagcac caggatcagc
    16141 ccgacccagc gcaggtacca cgccacctcc gagcgcacgg cgacgcgcgg cgcggcgatg
    16201 ccgaagcgcc ggcgcagttt ccggaatctc gtcgttatcg ccacgatccg atagggcttc
    16261 cgcgccgcgc agaaaacaaa aggccgctcg gcggcggcct catgcgtgac tgcctgcggc
    16321 gcggatcctc agcgcttgga gaactgcttg cgccggcgcg ccttgtgcag gccgactttc
    16381 ttgcgctcga cctcgcgcgc gtcgcgcgtc accagcccgg ccgcgctcag cgcgggcttg
    16441 aggcccgcgt cgtactcgat cagcgcgcgc gagatgccgt gacgcaccgc ccccgcctgg
    16501 ccgttctcgc cgccgccgaa gacgttgacc ttgatgtcga acgtcgagac gttcttggtc
    16561 acctcgagcg gctgtcgcac gatcatgcgc cccgtctcgc gtccgaagaa ctcatcgacc
    16621 ggtttgccgt tgacgatgat ctgccccttg ccgggcttga gaaatacgcg ggccaccgaa
    16681 ctcttgcgcc gccccgtgcc gtagtaatag tcgccaacca tggtcgtggt gtcctcagat
    16741 ctcgagcgtt tggggctgct gcgcggaatg cgggtgcgtc gccgcgccgt agaccttcag
    16801 cttcttcagc atcgcgtagc cgagcggccc cctgggcagc atgcccttga cggcctgctc
    16861 gagcgcccgc ccgggatggc gcacctgcag cttggcgaaa ttcgtttcgc gaatgccacc
    16921 cgggtggccc gtatggcggt agtacttctt gtccttcatc ttgcggccgg tgacgcggat
    16981 cttcgcggcg ttgacgacca cgatgtagtc ccccgtgtcg acgtgcggcg tgaactccgg
    17041 cttgtgcttg ccgcgcaggc gcgaggcgat tctcgccgcg aggcggccga gcaccttgcc
    17101 ctcggcgttc accaggtacc atccatgcac gacgtcctgg ggctttgctg caaaggtctt
    17161 catgggtggg gcgtagcggg aaaagctgcg gattctatgg gaccctcggg ccagcgtcaa
    17221 acccttgtta gaacaaaaaa aagcgcaatc cgaagattgc gcttattcca ccattggagg
    17281 agggtggaaa agattgcgtc gctaaagcgc aaggaggaga agacgcgccc gcgacgctct
    17341 gcagtatgcc gtaccatttg gtgcgctgca atatctttgt ctcatgtaca gaccattgtt
    17401 tcgcctgttc gaataaatct cttgaataga tcgatgctgc atagcacaat ccactgatta
    17461 cacgggagga tttgcctgat ggaatgcacg atccgctggt ccggtcacgg catgtcgttc
    17521 atcgccgaaa ccggaagcca ccacatcctc gcgatggatg gcgcgcctga cggcggcggc
    17581 cgcaacctcg cgccgcgccc catggagacc gtgctcgccg gtgccggcgg ctgcacggcc
    17641 tacgacgtcg tggtgatcct gaagaaaagc ggccaggacg tcaccggctg cgaggtgcgc
    17701 ctcgaggccg agcgcgcgac taccgatccc aaagtcttca cccgcatcca tacccatttt
    17761 ctcgtgcgcg ggcgcaacct gaagcgcaac gtcgtcgagc aggcgatccg cctgtcgcat
    17821 gagaagtact gctcggctac cgccatgctg gcaaagaccg ccgagatcac gcgcgacctc
    17881 gagatcgtgg aagactagag ccggtaggcg atcgtggtca tgacgcgggc cacggtacgc
    17941 atcgcgcggc gcgccggcgc gggcatgtcg gccgcgccca gccccaccgc cgtgccgcga
    18001 tggcgcgctt cgtcctcgcg catcgccgcg accacggcgc gcgtgggctt gtcctccggc
    18061 gcgagcttgt ccaggtggtt ggtcaggtgc tcctccacct gccgctccgt ctcggcgagg
    18121 aaggcaaggt tccagcggtc gccgagcagg ccggcgccga tccccatggc gagtgcgccg
    18181 gcgtaccaga gcgggttgag caggctcggc ctcccgccga gcgcgcggat gcgctccatg
    18241 ctccaggcga ggtgatcgcc ctcttcgcgc gccgcgcgct cgagcgacgc gcgcgtgtgc
    18301 gcgctgcgtg ccgtcagcgc ctgtccctgg tacagcgcct gcgcgcagac ctcgcccacg
    18361 tggttgaccc gcatcagcgc agcagagtgc gcccgctcgg ccgcgtccag gtccgcctcg
    18421 ggcatgcccg cagcggggtt cggccgctcg gcgtggtgca cgcccgcgac cgcgcgcagc
    18481 gcacggtcga actcgacgat gccgcggtcg agcaggtcgg tgaggcccgg cggcgtcatt
    18541 ggtgctccag ccccggcatg tagagcacgc gaccgccgtt cttgcgcacc agttgctccg
    18601 cgcgctcgca ggtcatcggc gccggctgct ggtcggcggc gacgcggtcg taccagggcc
    18661 ggcagtagcc gggcggcggc agctgctcgg gcgcgaggcc gccgaactgg ctcgggccgt
    18721 agtccatggc gagcgtgcgc ccgtcctgga acgagcccgg cgaaatcgcc ttgatcacgc
    18781 gcccgccgtg cttctcggca aggctgtgcg cgcgggcgca cggcatctct ggcggctgcc
    18841 attccggcgg caggtcggcg taccagatgc ggcacaggcc gagctgcggc a
//
LOCUS       DBNHKE010000171         9315 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_294693, whole genome shotgun sequence.
ACCESSION   DBNHKE010000171 DBNHKE010000000
VERSION     DBNHKE010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_294693"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..2015
                     /locus_tag="AC16O9_11665"
     CDS             66..2015
                     /locus_tag="AC16O9_11665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6517310.1"
                     /translation="MDFFAQQDEARRASRWLVLWYLVAAAFVVLCFNLAGALGYAVIA
                     IFGLLPLAGDEVVVWQGLARTYWHALVNIPPAFHLWVTAIVGFVVAGVSAWRIWKLSE
                     GGIAVAQMLSARYLERGRASATETRLLNVVEEMAIASGIAVPPVYLLGYDDAVNALVA
                     GHTPNEAVIIVTRGLVQKLTRDELQGVMGHEFSHILNGDMALNIRLAGLLGGLTVFGE
                     VGEALVYGAADTARGKSRENVGGEVFSAMIGTLIAFIAFPGSFAAEAIKAAISRRREL
                     LADAASVQFTRNPDGIAGALDAVSLLRAGTTLRGLYVDELSHMFFLPAVAHWWGFPTH
                     PPIEERIRRVHPRFQRDDYRRTRPGHHGGDRRVAVLDGGGSVVKVLGGIGAAAALVEA
                     PRHEHVDHARRLLERLPPTVKRRLQTPEGAAQVMCALLLEREGPVRERQLQLLGAEAE
                     QVAAARFELQGVGRRQFLLLLELALPALKQWAQKRRDAFLAMVQALVEADQKTTLGEF
                     VQATLLRQRLREGAGKPILTRYKKVEDVAADANAVISLIAHVSRGSTEVSHAKGQAIL
                     GFALPALLTLGELTAARMQQALERLRHLAPFQKPRLLKACVETAAADGPFRLAEMELV
                     RAVAATLDCPIPPVIQSLDPATLAA"
     gene            2012..4807
                     /locus_tag="AC16O9_11670"
     CDS             2012..4807
                     /locus_tag="AC16O9_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR6517311.1"
                     /translation="MKELQAALAAAVAGEVRFDAGARAAYASEASNYRQVPIGVILPR
                     SAEDVMAALAVCRAHGAPVLARGGGTSMCGQSVNVAVVIDCSKYMDRVLSIDAATKSA
                     RVEPGVVCDSLSAAAAGHGLVFGPDPATHSRCTLGGMIGNNSCGAHSVMAGKTVENIE
                     ALEVATYDGARFWCGPTDEAAFDRILSKNDRQAEIYRGLKLLADRYAERIRAGFPTIK
                     RRVSGYNLDQLLPENGFNVARALVGSEGTCALTLQARATLVENPRHRLLLVQGYTDVF
                     AAGDAVPQILAAGPIACEGLDEPIIGGLRERKLKLEDIGLLPEGRAWLMIEFGGDTLE
                     QAIDKASSLRGTLVTDPAQIARLWAIRETGASATALNLKGTPGPDPVVGWEDAAVDPL
                     RLGDYLREFQALVDRHGYRTSLYGHFGDGCIHARITFDLRTPAGLARWRGFLQAAAEL
                     VVKYGGSLSGEHGDGQARAEFLPAMYGPELMQAFREFKRLWDPLNRMNPGKLVNAAGD
                     VLRVDENLRLGPAYKPVTLQTRFAFRSDVGDGFARAAEHCVGMGKCRSKSGGTMCPSY
                     RGTGEERYSTRGRARLLWEMLQGEVITGGWQSEEVKEALDWCLACKGCRSDCPTHTDM
                     AAYKAEFLSHYYEKKIRPPAVQALARIGEWAPLASKAPGLANVLGALGRPVAGLSTTR
                     RMPRLAAQSLRARFVPQGAGERVILFDDTFNGYFRPATGLAAARVLAHAGCSVELPKA
                     HLCCGRPYYDAGMLDQAKAALGRVLEALEPDVPVVVLEPACLSVFRDELKQLFADDDR
                     AQALAARVHSLAEFLDARGYAPPRSFGRALLQPHCHQKALWGSAAEVRLLRAAGLEVE
                     APDLGCCGMAGSFGLKAAHEAASRRIAEIALLPRLRAAPPDTLVVADGFSCREQIEGL
                     SDRTAQHFAEVLAGALR"
     gene            4804..5409
                     /locus_tag="AC16O9_11675"
     CDS             4804..5409
                     /locus_tag="AC16O9_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR6517312.1"
                     /translation="MRALLAALLLAQAQPHASPLHEAIAQGKEPVAAVLVLGGKVDLE
                     ARNAARETPLHLAAEQGMAAVTRLLLDAGARVRARTATGETALHHATLNPDAAVALLL
                     LDAGADPRALNDHGESALHWAAYSGHEAVVRLLLERGADADAFNLDGNLALHGAAAGG
                     HAGTVRLLLPHTSHPGLRNLQGKTPRDLARERGHEDVAALF"
     gene            complement(5406..6407)
                     /locus_tag="AC16O9_11680"
     CDS             complement(5406..6407)
                     /locus_tag="AC16O9_11680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6517313.1"
                     /translation="MSLKLSILDQSPVISGHTPAQAIAATLELARQAERLGYHRYWLA
                     EHHAIAALADPCPEVLLARLGAETRRMRIGTGGVLLPYYSAFKVAEVFRMLESLYPGR
                     VDLGIGRAPGGDARTAHAVGGGQLPTAEDFPQRVWELVAHLDNALPDDHPHKRVRLQP
                     GGDTAPEVWLLGSSDFSGALAAQLGLRFAFAHFINPRGGDVVTRAYRERFQPSSREAS
                     PAAIVCVFVICAADDAEAGRLAASIDLRRLHMALGRDTPVPTLAEAERHEYSEEERRY
                     VLAQRERAVIGGPEKCRRELTALAERYAADEVMALTITGDYASRLRSYELLAEVFRL"
     gene            6464..6754
                     /locus_tag="AC16O9_11685"
     CDS             6464..6754
                     /locus_tag="AC16O9_11685"
                     /inference="COORDINATES: protein motif:HMM:NF018473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR6517314.1"
                     /translation="MRLALTVLLAALASQAQALQCSAQSGAHTAAPGRVVPLSLHVKY
                     WDYIGWKDPYAQQRFADRQRKLAQVIRARVVYTPPGHAPGARLPPLVCRRGL"
     gene            7067..8179
                     /locus_tag="AC16O9_11690"
     CDS             7067..8179
                     /locus_tag="AC16O9_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HIR6517315.1"
                     /translation="MLATQIRQKDGIFYFVSYPAKELLSRVRFISRFYGEGEQIAPSR
                     IAQDDDIAQFIARIERTDHAFQRSLSQAKVRQLRNFYETAVTQPPIPGTVLLFTSETL
                     RYQSAADGMGHLSEPSAKFLIIDGQHRLAALHFYMLDKPAEAAAIRVPCMIFDGRSED
                     FSTEMFVIINSTPTRINKSHLVDLYEKVSWAAPDRKFAARLVDKLYSEGDSPLRYRIN
                     RLGGRSQRDKWILQAELFNELHRWVRAEWRRIQVTGGSAKNVDKYYAAIRDFLKAAKA
                     AFGDTWGKDNYMVTKPVTLKAMIRVCTALAREDAEPAEGRLQRWEQRLAPWADMVREF
                     REEGFYERFPAKGEVERVARVQRDLARAAGIELKKT"
     gene            complement(8291..>9315)
                     /locus_tag="AC16O9_11695"
     CDS             complement(8291..>9315)
                     /locus_tag="AC16O9_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6517316.1"
                     /translation="LSSHFVWTNRSKESLALDVKHPLAKPILARLLERADVFVQNLAP
                     GAVERLGLGAAELRARHPRLVWCGISGYGPAGPYAAKRAYDLLVQCEAGLLSVTGTPE
                     APAKAGIPVADIAAGMYAMSSILAALLRRERDGVGATLDITMFESLGEWMGFPAYFTA
                     YGGTPPPRSGAYHATIVPYGPFAAGDGKTVFLSIQNEREFARFCDQVLGNAALAKDPR
                     FSSSPARVAHRDAMHAEIARVFGALGAAQVVQRLEAAGIANARLNTMQEFWDHPQLAA
                     RDRWREVGTEAGAVRAPKVPLNLSEFEPRMDDVPALGAHSRAILAELGYGEAEIDRLA
                     GEKAIA"
BASE COUNT         1382 a         3183 c         3379 g         1371 t
ORIGIN      
        1 gagctcctgc gcgtcaccgg cgcggtgctc gactgcccgc tgccgccgct gctcgactag
       61 cgggcatgga tttcttcgcg cagcaggacg aggcgaggcg cgcgtcgcgc tggctggtgt
      121 tgtggtacct cgttgcggca gccttcgtcg tcttgtgctt caacctggcc ggggcgctcg
      181 gctatgcggt aatcgcgatc ttcggcctgc tgccgctcgc cggggatgag gtcgtcgtct
      241 ggcaggggct cgcgcgcacg tactggcacg cgctcgtgaa catcccgccg gcgttccacc
      301 tgtgggtgac cgcgatcgtg ggcttcgtcg tggcaggcgt gtctgcctgg cgcatctgga
      361 agctgagcga ggggggaatc gcggtggcgc agatgctgag cgcacgctat ctcgagcggg
      421 ggcgggccag cgccaccgaa acccgcctgc tcaacgtcgt cgaggagatg gcgatcgctt
      481 cgggcattgc ggtgccgccg gtgtacctgc ttggctacga cgacgcggtg aatgcgctgg
      541 tcgcgggtca tacgccaaac gaggccgtga tcatcgtcac ccgcggactg gtgcagaagc
      601 tgacgcgcga cgagctgcaa ggcgtcatgg gccacgaatt cagccacatc ctgaacggcg
      661 acatggcatt gaacatccgc ctggcggggc ttctcggggg cctcacggtt ttcggcgagg
      721 tgggggaggc gctcgtgtat ggcgcggcag acacggcgcg cggcaagagc cgggagaacg
      781 tgggcggcga ggtattcagc gccatgatcg gcacgctgat cgcgttcatc gcgtttccgg
      841 gctcctttgc cgccgaagcg atcaaggcgg cgatctcgcg ccggcgcgag ttgctggccg
      901 atgcggcaag cgtgcagttc acgcgcaacc ccgacggcat cgccggcgcc ctggacgcgg
      961 tctcgctgct gcgcgcggga actacgttgc gtggactgta cgtcgatgag ctgtcgcaca
     1021 tgttcttcct gcctgctgtt gcgcattggt ggggcttccc gacgcacccg ccgatcgagg
     1081 agcgcatccg gcgggtgcat ccgcggttcc agcgcgatga ttatcggcgc acgcgtcccg
     1141 gacaccacgg tggcgaccgc cgcgtcgccg tgctcgacgg cggcggcagc gtcgtcaaag
     1201 tgctgggcgg catcggcgcg gccgcggcgc tcgtcgaagc gcccaggcac gagcacgtcg
     1261 accatgcccg gcgcctgctc gagcgcctgc cgcccaccgt gaagcgccgc ctgcagacac
     1321 ccgagggtgc cgcgcaggta atgtgcgccc tgctgctcga gcgcgagggt ccggtgcgcg
     1381 aacgccagct gcagctcctc ggcgccgagg ccgaacaggt cgcagcggcg cgcttcgagc
     1441 tgcagggtgt cggccggcgg cagttcctgc tgcttctcga gctcgcgctg ccggcgctca
     1501 agcaatgggc gcagaagcgg cgcgatgcct ttctcgccat ggtgcaggcg ctggtcgagg
     1561 ccgaccaaaa gacgacgctg ggcgaattcg tgcaggcgac gctgctgcgc cagcgcctgc
     1621 gggaaggcgc aggcaagccg atccttacgc ggtacaagaa agtggaagac gtggccgccg
     1681 acgccaacgc ggtgatctcg ctcatcgcgc atgtttccag gggaagcacg gaggtctcgc
     1741 acgccaaggg ccaggcgatc ctggggttcg ccctgccggc gctgctgacg ctgggcgagc
     1801 tcaccgcggc gcgcatgcag caggcgcttg agcgcctgcg ccacctggcg ccgttccaga
     1861 agccgcgtct cctgaaggcc tgcgtcgaga ccgcggccgc cgacggcccg tttcgcctgg
     1921 cagaaatgga gctggtgcgc gcagtcgcgg cgacgctcga ttgcccgatc ccgcccgtga
     1981 tccagtcgct cgacccggct acactcgcgg catgaaggaa ttgcaggccg cgctcgcggc
     2041 cgccgtcgcg ggcgaggtgc gcttcgacgc cggcgcgcgc gccgcctacg cctccgaagc
     2101 gtccaactac cgccaggtgc cgatcggcgt gattctgccg cgctcggccg aggacgtgat
     2161 ggccgcgctc gccgtatgcc gcgcgcacgg cgcgccggtg ctcgcgcgcg gcggcggcac
     2221 ctcgatgtgc ggccagagcg tgaacgtggc ggtggtgatc gactgctcga agtacatgga
     2281 ccgggtgctg tcgatcgacg ccgcgacgaa gagcgcgcgg gtcgagcccg gcgtcgtgtg
     2341 cgacagcctg agcgcggcgg cggccggcca cggcctggtc ttcgggcccg acccggcgac
     2401 gcacagccgc tgcacgctcg gcggcatgat cggcaacaat tcctgcggcg cgcactcggt
     2461 gatggccggc aagaccgtgg agaacatcga ggcgctggag gtggcgacct acgacggcgc
     2521 gcgcttctgg tgcgggccga cggacgaggc cgcgttcgac cgcatccttt cgaagaacga
     2581 ccgccaggcc gagatctatc gcggcctgaa gctgctcgcg gaccgctacg cggagcggat
     2641 ccgcgccggc tttccgacga tcaagcgccg cgtttcgggc tacaacctcg accagctgct
     2701 gcccgaaaac ggcttcaacg tggcgcgcgc gctggtgggc tcggagggca cctgcgcgct
     2761 gacgctgcag gcgagggcga cgctggtgga gaacccgcgc caccgcctgc tcctggtgca
     2821 gggctataca gatgtctttg ccgccggcga tgcggtgccg cagatcctgg ccgcggggcc
     2881 catcgcctgc gagggcctcg acgagccgat catcggcggg ctgcgcgagc gaaagctcaa
     2941 gctcgaggat atcgggctgc tgcccgaggg gcgcgcctgg ctgatgatcg agttcggcgg
     3001 cgacacgctg gagcaggcga tcgacaaggc gagcagcctg cgcggcacgc tggtcaccga
     3061 cccggcgcag atcgcgaggc tgtgggcgat ccgggagacc ggcgcctcgg ccaccgcgct
     3121 caacctgaag ggcacgcccg ggcccgatcc cgtggtcggt tgggaggacg ccgcggtgga
     3181 tcccttgcgc ctcggggact acctgcgcga gttccaggcg ctggtggatc gccacggcta
     3241 ccgcacctcg ctctacgggc acttcggcga cggctgcatc cacgcgcgca tcaccttcga
     3301 tttgagaacg cccgcgggcc tcgcgcgctg gcgcggcttc ctgcaggccg ccgccgagct
     3361 ggtggtgaag tacggcgggt cgctgtcggg cgagcacggc gacggccagg cgcgcgcgga
     3421 gttcctgccg gcgatgtacg gccccgagct gatgcaggcg tttcgcgagt tcaagcgcct
     3481 ctgggatccc ttgaaccgca tgaaccccgg aaagctcgtc aacgccgcgg gcgacgtgct
     3541 gcgcgtggac gagaacctgc gcctggggcc ggcctacaag ccggtgacgc tgcaaacgcg
     3601 ctttgccttc aggagcgacg tgggcgacgg cttcgcgcgc gccgccgagc actgcgtcgg
     3661 catgggcaag tgccgatcga aatcgggcgg caccatgtgc ccgagctacc gcggcacggg
     3721 cgaagagcgc tactccacgc gcgggcgcgc gcgcctgctc tgggagatgc tgcagggcga
     3781 ggtgatcacc gggggctggc agagcgaaga agtgaaggag gcgctcgact ggtgcctcgc
     3841 ctgcaagggc tgccgcagcg actgcccgac gcacaccgac atggcggcct acaaggcgga
     3901 gttcctgtcg cattactacg agaagaagat ccgaccgccc gcggtgcagg cgctcgcgcg
     3961 catcggcgag tgggcgccgc tcgcttccaa ggcgccgggc ctggccaacg tgctgggcgc
     4021 cctgggccgg ccggtggccg ggctgagcac cacgcgccgc atgccgcgcc ttgccgcgca
     4081 gagcctgcgg gcgcggttcg tgccgcaggg cgccggcgag cgcgtgatcc tgttcgacga
     4141 cacgttcaac ggctacttcc ggccggcaac aggactggcc gcggcgcgcg tgctggcgca
     4201 tgccgggtgc agcgtcgagc tgccgaaagc gcacctgtgc tgcgggcgcc cgtactacga
     4261 cgcggggatg ctcgaccagg cgaaggcggc gctcggtcgt gtcctcgaag cacttgagcc
     4321 ggacgtgccg gtcgtcgtgc tcgagcccgc gtgcctctcg gtgttccgcg acgagctcaa
     4381 gcagctcttt gcggacgacg atcgtgcgca ggcgctcgca gcgcgggtgc attcgctcgc
     4441 cgagttcctc gacgcgcgcg gctatgcgcc gccgcgcagc tttggccgcg cgctgctgca
     4501 gccgcactgc caccagaagg cgctctgggg gagcgccgcg gaggtgcggc tcctgcgcgc
     4561 cgccggcctc gaggtcgaag cgccggatct gggttgctgc ggcatggcgg gctccttcgg
     4621 cctgaaggcg gcgcacgaag cggcctcgcg ccgtatcgcg gagatcgcgc tgctgccgcg
     4681 gctgcgcgcc gcgccgcccg acacgctggt ggtggccgac ggcttttcct gccgcgagca
     4741 gatcgagggt ctgtcggatc gtacggcgca gcacttcgcg gaggtgctgg cgggcgcact
     4801 gcgatgaggg cgctgctcgc ggcgctgctc ctcgcccagg cacagccgca cgcctcgccc
     4861 ttgcacgagg cgatcgcgca gggcaaggag ccggtcgccg ccgtgctcgt gctgggcggc
     4921 aaggtggacc tcgaggcgcg caacgcggcc cgcgagacgc cgctgcacct ggcggccgag
     4981 cagggcatgg ccgcggtgac gcgattgctc ctcgacgccg gcgcgcgggt gcgcgcgcgc
     5041 acggcgaccg gtgaaaccgc gttgcaccat gcgacgttga accccgatgc ggcggtcgcg
     5101 ctgctattgc tcgatgcggg cgcggacccc cgggcgctca acgatcacgg cgagtcggcg
     5161 ctgcactggg cggcctacag cggccacgag gcggtggtgc gcctgctgct cgagcgcggt
     5221 gcggatgcgg acgcgttcaa cctcgacggc aatctcgcgc tgcacggcgc ggcggccggc
     5281 gggcacgctg gcaccgtgcg gctgcttctg ccgcacacga gccatccggg cctgaggaat
     5341 ctgcagggca agacgccgcg ggatctcgcg cgcgagcgcg ggcacgagga tgttgccgcg
     5401 ctgttctaga gcctgaacac ctcggccagc agctcatagg agcgcagccg cgacgcgtaa
     5461 tccccggtga tggtgagtgc catcacctcg tcggcggcgt agcgttcggc gagcgccgtg
     5521 agctcgcgcc ggcatttctc gggtccgccg atcaccgcgc gctcgcgctg cgcgagcacg
     5581 tagcgtcgct cctcctccga atactcgtgc cgctcggcct cggcgagcgt cggcaccggc
     5641 gtatcgcgcc cgagcgccat gtgcaggcgg cgcaggtcga tcgaggcggc gagccgcccg
     5701 gcttcggcgt cgtcggcggc gcagatcacg aacacgcaga cgatggcggc gggcgatgct
     5761 tcccgcgacg atggctggaa gcgctcgcgg taggcgcgcg tcacgacatc gccgccgcgc
     5821 gggttgatga agtgggcgaa ggcgaagcgc aggccgagct gcgcggcgag cgcgccgctg
     5881 aaatccgatg accccaggag ccagacctcg ggcgccgtat cgccgcccgg ctgcagccgc
     5941 acgcgcttgt gcggatggtc gtcgggcagg gcgttgtcga gatgggcgac aagctcccag
     6001 acccgctgcg ggaagtcctc cgccgtcggc agctggccgc cgccgacggc atgcgcggtg
     6061 cgggcatcgc cccctggagc gcggccgatg cccaggtcga cgcgaccggg gtaaagggat
     6121 tccagcatgc gaaacacttc ggccaccttg aacgcactgt aatagggcag gaggacccct
     6181 ccggtgccga tgcgcattcg ccgggtctcc gccccaaggc gggcgaggag cacctccggg
     6241 cagggatcgg caagcgctgc gatggcatgg tgctcggcga gccaatagcg gtggtagccg
     6301 agccgctcgg cctgacgggc gagctccagc gtggcggcga tcgcctgcgc cggggtatgc
     6361 ccggagatca ccggcgactg gtcgaggatg gagagtttca aggacacccc gcattatcgc
     6421 gttgcagcgg taagcgccgc ccccgccgtt tcgactgaac cgcatgcggc ttgccctgac
     6481 cgtgctcctc gcggcgctgg cttcccaggc gcaagcgctg caatgcagcg cgcagtccgg
     6541 ggcccatacg gctgcgccgg ggcgcgtggt gcccctctcc ttgcacgtga agtactggga
     6601 ctacatcggc tggaaggatc cgtacgccca gcagcgcttt gccgaccgcc agcgaaaact
     6661 cgcgcaggtc atccgtgcgc gcgtcgtcta caccccccca ggtcatgctc caggggcaag
     6721 acttccgccg ctggtatgcc gccggggcct ttgaaaagga gggggccagg atcaatgccc
     6781 ggccgccggt ggcgcatatc gccctgcgcc tggaggcggg ccgatcccgt tcacggccgg
     6841 caggatcagg caacaacggg ccctgcccct gctgccgaag gcggtcttgg ggcactgcgg
     6901 ggtggccgct ttcgtgcaaa accggcgcac ggctaaggtg ctacaggccc tgatgctgcc
     6961 ggtctgcccg gggtgggatt gaaatcccgc cctgcggggc ccggcagcct tcggtatcat
     7021 tcgccgggac ttgatagttg gggcgacccg cccggaggca gcaaccgtgc tcgcgacgca
     7081 gatacgccag aaggacggga tcttctactt tgtcagctac ccggcgaagg agctattgtc
     7141 ccgggtacgt ttcattagcc gattctatgg cgagggcgag cagatcgccc cctcgcgcat
     7201 cgcccaggac gacgacatcg cgcagttcat cgcgcgcatc gagcgcaccg accacgcgtt
     7261 ccagcgctcg ctctcgcagg ccaaggtgcg ccagctgcgc aacttctacg agaccgcggt
     7321 gacgcagccg ccgattcccg ggacggtgct gctgttcacc tcggagaccc tgcgctacca
     7381 gtccgcggcc gacggcatgg ggcacctgtc ggagccgagc gccaagttcc tgatcatcga
     7441 cggccagcac cgactggcgg cgctgcattt ctacatgctg gacaagcccg ccgaagccgc
     7501 cgccatccgc gtgccgtgca tgatcttcga cggccgcagc gaggacttct ccaccgagat
     7561 gttcgtcatc atcaattcca cgccgacgcg catcaacaag agccacctcg tggatctcta
     7621 cgagaaggtg tcgtgggcgg cgccggaccg caagttcgcc gcgcgcctgg tcgacaagct
     7681 ctacagcgag ggcgacagcc cgctgcgcta ccgcatcaac cggctcggcg ggcgcagcca
     7741 gcgcgacaag tggatcctgc aggcggagct gttcaacgag ctgcaccgct gggtgcgcgc
     7801 cgagtggcgc cggatccagg tcaccggcgg cagcgcgaag aacgtcgaca agtactacgc
     7861 ggcgatccgc gacttcctca aggccgcgaa ggccgccttc ggcgatacct ggggcaagga
     7921 caactacatg gtcaccaagc cggtgaccct gaaggcgatg atccgcgtgt gcacggcgct
     7981 tgcgcgcgag gacgcggagc ccgccgaagg gcgcctgcag cgttgggagc agcgcctcgc
     8041 gccctgggcc gacatggtgc gcgagttccg tgaggaaggc ttctacgagc gcttccccgc
     8101 caagggcgag gtcgagcgcg tggcgcgtgt gcagcgcgac ctggcgcgcg ccgcgggcat
     8161 cgagctgaag aaaacctagc gcgggcgttc agcccatccg cgggtttcgc ctttcaaata
     8221 catctcggct tttctggcgg gaagattcac cccgccagaa aagccgagac gcattgaacg
     8281 gcagcagcac ctatgcgata gccttttctc ccgctaagcg gtcgatctcc gcttcgccgt
     8341 agccgagctc cgcgaggatc gcgcggctgt gcgcgccgag cgcgggcacg tcgtccatgc
     8401 gcggctcgaa ctccgacagg ttgagcggca ccttgggcgc gcgcaccgcc ccggcctcgg
     8461 tgcccacctc gcgccagcga tcgcgcgccg cgagctgcgg gtggtcccag aactcctgca
     8521 tggtgttgag ccgcgcgttg gcgatgccgg cggcttccag ccgctgcacc acctgcgccg
     8581 cgcccagcgc gccgaacacg cgcgcgatct cggcgtgcat ggcgtcgcgg tgcgccacgc
     8641 gcgcggggct tgaggaaaag cgcgggtctt tcgccagcgc cgcgttgccc agcacctggt
     8701 cgcagaagcg cgcgaactcg cgctcgttct ggatgctgag gaacactgtc ttgccgtcac
     8761 ccgccgcgaa cggcccgtag ggcacgatag tggcgtgata ggcccccgag cgcggcggcg
     8821 gcgtgccgcc gtaggcggtg aagtacgcgg gaaaccccat ccattcgccg agcgactcga
     8881 acatggtgat atcgagcgtc gcgcccacgc cgtcgcgctc gcgccggagc agcgcggcga
     8941 ggatcgagga catcgcgtac atgccggcgg cgatgtcggc gaccgggatg ccggccttgg
     9001 cgggcgcctc gggggtgccg gtcaccgaga gcaggcccgc ctcgcactgc acgagcaggt
     9061 cgtaggcgcg cttggccgca taagggccgg cgggaccata gccggagatg ccgcaccaca
     9121 ccaggcgcgg atggcgcgcg cgcagctcgg ccgcgcccag gcccaggcgc tcgacggcgc
     9181 cgggcgcaag gttctgcacg aacacgtcgg cgcgctcgag gagccgcgcg aggatcggct
     9241 tcgccagcgg gtgcttcacg tccagggcga ggctttcctt ggagcggttg gtccagacga
     9301 aatgcgacga cagcc
//
LOCUS       DBNHKE010000172        26527 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_558941, whole genome shotgun sequence.
ACCESSION   DBNHKE010000172 DBNHKE010000000
VERSION     DBNHKE010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 26527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 26527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26527
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_558941"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1090)
                     /locus_tag="AC16O9_11700"
     CDS             complement(176..1090)
                     /locus_tag="AC16O9_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6517317.1"
                     /translation="MKETRDYAIVFADLAESTRIFRELGDAAARDLTQVFYGRVAAVL
                     PQHQGRIVKTLGDGVMCAFPDADRAVLAMGALHAGMAAVRAPEQPLRVHSGISWGSVI
                     VEGADVFGTVVNVAAYLAAIARANQILATQAAVEQLSPPIRATARAAYAARLKGDDRE
                     SLVHEVVWQPDRAGVTNVNSTAVGVLPADEGALQFLFGAAMHRLNAFNPRLSFGRDAG
                     NDIVVADDYASRSHAVVEVDTMRFKFTDRSANGTYVALDGTPGEVRVLRGETVLHGSG
                     RISLGRPLSDPKAQPIAFRRDQRALYRV"
     gene            1387..1941
                     /locus_tag="AC16O9_11705"
     CDS             1387..1941
                     /locus_tag="AC16O9_11705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6517318.1"
                     /translation="MTAKSPSTRVDASVRDAAPGDLPAVQAIYAQHVLRGLASFEEVP
                     PDLEEMRRRHADVTGRGLPYLVAARQGEVLGFGYCAPYRARSAYRYALEDSVYIREGS
                     AGQGLGSRILAELIARCERLGYRQVIAVIGDSANAASIALHARFGFLRVGTLRSTGYK
                     FGRWVDSVLMQRPLGPGDGTPPAT"
     gene            complement(1934..2686)
                     /locus_tag="AC16O9_11710"
     CDS             complement(1934..2686)
                     /locus_tag="AC16O9_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6517319.1"
                     /translation="MIDALTSLASAPALAFCVAVVFAAYTLRGATGFGAGVVAIPLLA
                     LFMPLTVVIPVITTLGIVASLGQSAQEFRFVDWRALRGLALPSAVGLGLGLWLFASLD
                     QALLVKAFALFIILYGSWGLLPRRPVWRLPAPALAAVAGGSGGLVATLFGGMAGPFYV
                     IYLRALALDKRRFRASMSSVLLCLGLVRAGGYGTLGFFDRRAIAALALLAPVMVGAMV
                     VGDRWHARLDQAKFERVVAVLLAGSGIALLFK"
     gene            complement(2753..3733)
                     /locus_tag="AC16O9_11715"
     CDS             complement(2753..3733)
                     /locus_tag="AC16O9_11715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6517320.1"
                     /translation="MKALLCKQFGPPESLVLEDVPSPRPGADEVVIGVRAASVNFPDV
                     LIIQGKYQFRPPLPFSPGSELAGVVKEVGAGVKNAKPGDKVIAYTTFGAFAEEVNVNA
                     GRLLPMPEGMSYDEAASFVLTYGTTDHALRDRGQLKAGESLLVLGAAGGVGVAALEIG
                     KAMDARVIACASSDDKLALCREHGADETINYATEDLRERIKALTAGKGVDVIYDAVGG
                     PYTEPAFRSIAWRGRHLVIGFAAGDIPKLPLNLPLLKGASVVGVFWGDFTRREPQVFA
                     ESARQLSRWYREGRLRPHVSARYPLEKAAEAMNLLASRKAKGKVVVTIGA"
     gene            complement(3752..4492)
                     /locus_tag="AC16O9_11720"
     CDS             complement(3752..4492)
                     /locus_tag="AC16O9_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HIR6517321.1"
                     /translation="MDLNLKGKRALVTGGSKGIGWACANALADEGCEVMIAARNPPAA
                     RRHKFRATDLSQRGAAEQLAEWAGGLDILVNNAGAIPGGDLLTVDEETWRRAWDLKVF
                     GYINLTRQVYARMKSRKSGVIVNILGNAGERLDASYIAGSTANAGLMAFTRTLGGASH
                     ADGIRVVGINPGPVATDRLKSLLRQRAEAKFGDPERYAELFAGMSFGRPATPEEIAWM
                     AAFLASERSAYTSGCIVTIDGGLAARSS"
     gene            4512..5348
                     /locus_tag="AC16O9_11725"
     CDS             4512..5348
                     /locus_tag="AC16O9_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517322.1"
                     /translation="MPTITTDDGVKLHYEEAGRGTPVVFVHEFAGDLRSWEPQFAHFS
                     RRYRCIAYNARGYPPSDVPEDFERYSQDRARDDVRAVMDALKIDKAYVVGLSMGGFAS
                     LHFGIKYSARALGIVVGGCGYGSVKATRDAFQEDSRANARQMMSEGMERFAATYGHRP
                     TRMQLKAKDPRGFDLFIRYLEEHSALGSANTMLGYQARRPSLYDLVDEMKAITAPTLI
                     MTGDEDDGALEPSVLMKRTIPHAGLVVLPRTGHILNLEDPALYNRIVEDFFHQVESGR
                     WA"
     gene            5345..6133
                     /locus_tag="AC16O9_11730"
     CDS             5345..6133
                     /locus_tag="AC16O9_11730"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6517323.1"
                     /translation="MSAGRLVVEKRGAVGWIVFDQPAKKNAINDAMWRGIPEAMARFD
                     ADADVRCVAFRGAGTEAFAAGADISEFEKIRAHRAAVGEYDGLLDRVLLSIQGSLKPS
                     VALIHGFCMGGGLEVALACDLRYCGASAQFGIPAAKLGLAYNVEGHKRLLETVGHARA
                     REIMFLGRRYPAAEALAMGLVHRVLPDAELEGHVDGVIATLIANAPLSIANSKTILEE
                     YAKSVGLADHQRMQEAMDRCTKSADYEEGRRAFMEKRKPAFKGR"
     gene            6135..7214
                     /locus_tag="AC16O9_11735"
     CDS             6135..7214
                     /locus_tag="AC16O9_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR6517324.1"
                     /translation="MKIESIRTRAVAAPMKRPLATSTGALTASSLLLLDLQTDAGPVG
                     RSYLFGIGAHNLKPIAALVEAMAEMLKGDAVAPVDIEKKLRQRYTLLGVHNIVLFAMS
                     GIDMAAWDALGQAQGLPLVRMLGGTPRPIPAYNSKGLGIMPLEPLAKEAVELVKEGFA
                     AVKLRLGRPHAADDLAALRAVKKAIGTDITLMVDFNQGLTVAEAVKRGRMIDDEGGVT
                     WIEEPVRADDFAGNARIAREVATPIQIGENFMGPEQMAQALAAGACDYVMPDAQRIGG
                     VTGWMRAAALAQAAGMEMSSHLFPEASCHLLAVTPTCHWLEYVDWADAILEDPVQVKN
                     GHALIPERPGLGMRWDEKAVTKYQL"
     gene            7214..8869
                     /locus_tag="AC16O9_11740"
     CDS             7214..8869
                     /locus_tag="AC16O9_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR6517325.1"
                     /translation="MFTRHFAHWPPGVPKTLSVPRTSLFFNLEVSARRYPAKAALHYY
                     GTDVSYAELLRSAEALAGFLQQRCGVQRGDRVLLYAQNCPQFVIGYYAILRADAVVVP
                     VNPMNRHDELAFYISDSDATVILSTQDLAAEIEPHLGQGLRHCVLGAYSDYLREPTDL
                     GVPEAVRAPRSAAATWVEALAAGYAPGPHTAGPDDLAVLPYTSGTTGHPKGAMLLHSN
                     LLATSTGACAWIQSSSEAVVLGVLPMFHLTGMQANMNVPIQLGATSVLMTRWDRSVAA
                     ELIQRRRVTSVTGITTMMVDFLANPELGRYDLSSLVRVGGGGAAMPEAVAAKLQERIG
                     LPFIEGYGLTETAAPSHANPPHRPKRQCAGIPYFDTDSRVIDPVSLRELGPKEVGEIV
                     THGPQVFQGYWKQPKATADAFIEHDGKKFFRTGDLGYYDEDGYFFITDRLKRMINASG
                     FKVWPAEVEAMLYAHPDIQEACVIGTRDAHRGETVKAIVVLKPAARGKLRPEDISDWA
                     KAHMAAFKYPRVVEFVEQLPKTGTGKIFWRRLQEEENKRCGAG"
     gene            8854..9291
                     /locus_tag="AC16O9_11745"
     CDS             8854..9291
                     /locus_tag="AC16O9_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR6517326.1"
                     /translation="MRCGLALLLLTALPAWAQVTAEQPWSRATPPGANVGVGFLRLKN
                     AGAAAERVVGASSPVAGRVEMHVTTREGDVMKMRQVQSFEIPAGGSFELRPGGAHLML
                     MGLQRPLAQGERVPLTLKLENGGTLQVELAVEPLGARQPAHRQ"
     gene            complement(9381..10550)
                     /locus_tag="AC16O9_11750"
     CDS             complement(9381..10550)
                     /locus_tag="AC16O9_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR6517327.1"
                     /translation="MDLSKASFWGYRRENQRIGVRNHVLILPVDDLSNAAAQAVENNV
                     KGTLAIPHPYGRLQFGADLELHFRTLIGAGSNPNVAAVVVICIEDGWAKKVADGIAAT
                     GKPVSYFGIEQHGDHDTIMRASKAAKEYVQWASELRREERPLKDLWVSTKCGESDTTS
                     GCGANPTVGNAFDKLYPRGVTMVFGETTELTGGEHLVAARCRNDAVRTKFQTVFDRYQ
                     EVIERHKTSDLSDSQPTKGNIAGGLTTIEEKALGNIQKIGRLCKVDGVLDKAEMPTGP
                     GLWFMDSSSAAAEMVTLCAAAGFTVHLFPTGQGNVIGNPILPVIKLSANPRTVRTMSE
                     HIDVDVSGLLRKELTPDQAGDKLLECMFRTANGRLTAAEALGHREFVMTRLYESA"
     gene            complement(10628..11641)
                     /locus_tag="AC16O9_11755"
     CDS             complement(10628..11641)
                     /locus_tag="AC16O9_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR6517328.1"
                     /translation="MDSLAPVQIALGVVIALVLVALVFDFMNGFHDAANSIATVVSTR
                     VLKPYQAVMLAAAFNFLALFVFQLKVATTVGKGIVDQGVVDHYLVFGALIGAISWNLI
                     TWWYGIPSSSSHALIGGLVGAAVAKAGTVSLLPAGILKTVAFILIAPLMGFVLGALLM
                     LLVAWLFVRSSPSRVDRWFRRLQLVSASLYSLGHGGNDAQKTIGIIWMLLIAAGLSGA
                     DSPPVWVVIACYVTIAAGTAFGGWRIVKTMGQRITKLKPVGGFCAETGGAMMLFVATS
                     LGIPVSTTHTITGSIVGVGSANRVSAVRWGVAGNIVWAWILTIPCSAFIAAIAWGVGR
                     LLF"
     gene            complement(11650..12276)
                     /locus_tag="AC16O9_11760"
     CDS             complement(11650..12276)
                     /locus_tag="AC16O9_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR6517329.1"
                     /translation="MLSRLMPREGRFFDHFNAHAERIVEGSRELAGMMENFNDLEGYT
                     QRIDTAERAADKITHETIAMLHRTFITPFDREHIHQLITRMDDILDLIQDVAESVSLY
                     DLRRITPEAKQLAGICQMSCERVGSVVKLLTNVKRTEAIFKICEEIDRLESDADRVLR
                     SALSKLFREENDLKQVIKMRVIYDLLESITDRCEDVANIVEGIVLENS"
     gene            complement(12311..13462)
                     /locus_tag="AC16O9_11765"
     CDS             complement(12311..13462)
                     /locus_tag="AC16O9_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6517330.1"
                     /translation="MPGPLAGIRVLEFEAIGPGPFAGMLLADMGADVLLVDRAAEADL
                     GLKRERWNDVMMRGRRSVTLDLKSPKGAEAALELVARADALIEGFRPGVMERLGLGPQ
                     AALKRNPKLVYGRMTGWGQDGPLAPRAGHDINYIALAGVLNAFGRKGDAPVPPLNLVG
                     DFGGGGMLLGFGVACALVEAGRSGKGQVVDAAMVEGASLLAAMFSGMLAAKRWSEARG
                     ENFLDGGAPWYDVYPTRDGKYVAIGAIEARFYEELIERLGLSGQTLPGQHERERWPEL
                     RERFARAIAAKTRDEWAQAFEGSDACFAPVLSFSEAKRHPHNVARGSFIELAKVEQPA
                     PAPRFSRTRATVRRAPPERGEGGREALAEWGFDGPQIETLRSLGLGFRD"
     gene            complement(13470..14300)
                     /locus_tag="AC16O9_11770"
     CDS             complement(13470..14300)
                     /locus_tag="AC16O9_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR6517331.1"
                     /translation="MGALEPDAPLSPAAGHYSYEHEGDRQFATTLARGLEVLRCFTPL
                     EPMLGNKEISVRTGLPKPTVSRLTYTLTKLGYLRHNMRLGKYQLGSAVLSIGYPLLAS
                     MSVRQVARPFMKDLAEYCSGAVSMGIRDRMSMVYVESCRSSGNGFAILPDIGTAVPIA
                     QSVIGRAFIASCTPPEREAVLNQMKVKEPEMHRKNRPQIDKAMEDIRSRGFCVSIGEL
                     LRDVHAVGVPMRRTVDGEVVAFNCAVPAFLLKKGQLEEDIGPRLVAMVRNIEAALGIH
                     "
     gene            14432..15199
                     /locus_tag="AC16O9_11775"
     CDS             14432..15199
                     /locus_tag="AC16O9_11775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517332.1"
                     /translation="MDQSAFDLSGKVALVTGSTRGIGKSIAEEMARAGAKVVVSSRKA
                     EACEQVRAEFEKRGYEVLARPCNVSRKDELQALVDAALKRWGRIDVAVSNAASNPYYG
                     PLAGIPDEALDKIFHNNVKSVLWLAALTLPGMAERGGGSFISVGSIGGIVASTVIGAY
                     GMSKAADHMLVKNLAAEWGPKNVRVNAIAPGLIKTDFAKALWEDEKRRKEREAATPLR
                     RLGEPKDIGAIAVFLASEAAAFITGQTIVADGGVTIL"
     gene            15196..16071
                     /locus_tag="AC16O9_11780"
     CDS             15196..16071
                     /locus_tag="AC16O9_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR6517333.1"
                     /translation="MTRSTLRSVLAAGEFVVAPGVFEMLSARIADRLGFKALYMTGYG
                     ISASHLGLADAGLVTYRDMVERARTIAQGTATPLIADADTGFGGLLNVRETVRGYEAA
                     GVQAIQIEDQAIPKKCGHTPNRRVVPLADAVKRIEVAVAARKSSDFLIVARTDARTGL
                     GLDEAIRRARAFARAGADILFVEAPESEAEFARVGAELAGEAKLLANMVPTGRSPMLS
                     ADKLRKFGFSIAIYPSAGMSAACAALEGAYAHLLARGSLEGSPVAAYDMQRMHELVGF
                     ADIWEFERRHVEPQD"
     gene            16076..17446
                     /gene="leuC"
                     /locus_tag="AC16O9_11785"
     CDS             16076..17446
                     /gene="leuC"
                     /locus_tag="AC16O9_11785"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6517334.1"
                     /translation="MAGRTLFDKLWDEHVVEDLGDGVQLLHVDRHLLNELSGHRGQLE
                     IAKRGLSMRNPELSIATLDHVVSTAPGRRGGDAPWAERMIEVFRAETRRAGVPLHDFG
                     DDGQGIVHVVGPELGFTLPGALLVCGDSHTCTHGALGALAWGIGTSELAHVLATQTIV
                     QQKPKSMRVRFEGALARGVGAKDMILALIGRIGTAGGVGYAVEYAGAAIRALEVEGRM
                     TVCNLSIELGAKIGMVAPDERTYAYLEGRRYAPRNGLWREALRHWRTLPSDEEATFDL
                     EHELDCAALAPQITWGTSPEDVIAVDGVVPDAKPAALEYMGLAAGRPIAGTRVDRVFI
                     GSCTNARLPDLRLAADLVRGRKAAPHVKAWVVPGSQRVKREAEAEGLHEVFGAAGFEW
                     RDPGCSMCVGANGDLVEPRARCVSTSNRNFVGRQGPQARTHLASPATAAACAIAGEIA
                     DARCLS"
     gene            17443..18054
                     /gene="leuD"
                     /locus_tag="AC16O9_11790"
     CDS             17443..18054
                     /gene="leuD"
                     /locus_tag="AC16O9_11790"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6517335.1"
                     /translation="MTRFTTLTALAAPFPQKNVDTDTIIRIERCTGTPKAELGKYAFE
                     MARYLPDGSDNPDFVLNQPRYRGARILVCGEFFGTGSSREMAVWALAGLGMRCLIAPS
                     FGEIFRGNCFQNGVLPIALPAAAVDALMRRAEAPGAAPFTVDLERQTVNGEYRFEVDA
                     RRKKMLLEGLDEIALTLSLEPRIAAYQAADRVRRPWIYDFTRS"
     gene            complement(18041..19615)
                     /locus_tag="AC16O9_11795"
     CDS             complement(18041..19615)
                     /locus_tag="AC16O9_11795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6517336.1"
                     /translation="MNEYDYVVVGGGSAGCALAARLAEDPASRVCLIEAGGAGRSLLI
                     DTPALLALTVPRRLHNWAFETEPQSGLAGRRGYQPRGKALGGSSAINAMIYVRGHPRD
                     YDDWAAAGNPGWGWRDVLPAFLRAEHNERGSDDFHATGGPLNVADLRSPNPVSLAFVE
                     AGVEAGHARNADFNGATQWGVGLYQVTQRNGRRWSAARAFVDAAVARGNLVLRTGTHA
                     LRLVLEGKACRGVETSRGTVHARRETIVACGAFGSPQLLLLSGIGPRHAIEPHGIALR
                     HELPGVGENLQDHPDYVIGYLSSAPGLIGLTARGLMNLAAGIGAFRREGRGLLASNVA
                     EAGGFLGTAPGLERPDLQLHFCIGLVADHGRDTTPRYGFSCHVCLLRPKSRGRVALRS
                     ADPLAPPLIDPAFLSEKGDAAQLARGVELTLQILRQPALAPFRGRNVFGEDWITGEAL
                     VDLVRQRADTVYHPAGTCRMGRGEDAVVDAHLRVRGIENLRVADASIMPTLIGGNANA
                     PAIMIGERCAEFVRSA"
     gene            19667..20851
                     /locus_tag="AC16O9_11800"
     CDS             19667..20851
                     /locus_tag="AC16O9_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517337.1"
                     /translation="MDLNYSQEELAFRDQVRGWLKQNLPADLRDKIAAYEELSRDDLL
                     RWHKILAAQGWVAPSWPKEWGGTGWSVVQRYIFEEECGYAGTPPLIPFGLSMCGPVLL
                     KFGTEEQKKTFLPRIYNGDDFWCQGYSEPGSGSDLASLKTKAARSGEHYVVNGQKIWT
                     TLGHYADWIFCLVRTDSSAAKRQEGISFLLIDMKSPGITVRPLILMDGGHEVNEVFFE
                     DVKVPVANLVHKEGEGWTVAKYLLGHERMNTGRIGASKRELAKLKEFAAQQKKGGRSL
                     LEDTRFRDRLSRLEVELMALEITNLRFLDRMRRTGQPPGADVSMLKIKGTEIQQGLTE
                     LMMQAVGPLAQPFKAVNGDADFDAFTAAIAPRYCNFRKTTIYAGSNEIQRNIIAKMTL
                     GL"
     gene            20854..21954
                     /locus_tag="AC16O9_11805"
     CDS             20854..21954
                     /locus_tag="AC16O9_11805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517338.1"
                     /translation="MNFDYNEEQQLLADSVRRFIQKDYEFEARRKIVDSAQGTSEKVW
                     GTLAEMGLTGIPFSPDYGGFGGGAIDLMSVMEAFGEGLVVEPYLATLLGAQCVARAGS
                     DAQKKALLPQVVEGKLKMAFAFSEKGARYDLAPAAVTARKAGSGWTLEGEKAVVLGAP
                     AAGKIVVSAKAPAGPSLFLVDAKGVKLKPYRTLDAMRAADVALSGAAAELLGAEGKAL
                     AVIEEVIDFGTALVCAEAVGAMKFACDTTLEYLKTRKQFGVPIGTFQALQHRMVDMFV
                     AYEQAKSMACLACSKVDGAVDAKERAHAVSAAKIKIADTARLISQEAIQLHGGMGMSE
                     ELKVSHSFRRLTMVAQQFGDADHHLERFAAQG"
     gene            22000..22641
                     /locus_tag="AC16O9_11810"
     CDS             22000..22641
                     /locus_tag="AC16O9_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR6517339.1"
                     /translation="MASRTVVIAGATGLVGREILAGLLADPSVSAVHSLGRRKLGVEH
                     PKLTSHVVDFGALPALPAADEVYLALGTTIKVAGSQAAFRAVDYDANLAVAKAALAAG
                     VTKVGLVSSMGADSKSGIFYSRVKGELEDALSEMPFAGLVIARPSMLLGNREELGQPV
                     RRGEEVASVLAKIGGFLIPANYKPIAASAVAKALLSRVPSASGKAVLLSGSMR"
     gene            22849..23676
                     /locus_tag="AC16O9_11815"
     CDS             22849..23676
                     /locus_tag="AC16O9_11815"
                     /inference="COORDINATES: protein motif:HMM:NF018352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HIR6517340.1"
                     /translation="MKKFMGLLLAVFIHALFFQTALAQDGKTALVPLPSVEDFTRGND
                     GWSFGLGLGVEYESAYEGSDEFGFEVDPAGAVQWRTGDDIFYWAGETLGWRGLRSKTW
                     LFDAAVAFDEGREESDSDDGRLNGLGDTDEGAELVLQARRAFDADWRYWLDGRVVTGE
                     NGSLGIFGVGRRFGDQKDGTGSEIAIVVVYHDSDRANTDFGINAAQSAASGLNQTRLS
                     GGFRSIGFHYNYRDYINKNWQIFGEVLYERYGSDIEDSPIARNNYEAEVGVGFIYVF"
     gene            complement(23798..24385)
                     /locus_tag="AC16O9_11820"
     CDS             complement(23798..24385)
                     /locus_tag="AC16O9_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6517341.1"
                     /translation="MKKAKKRPAQNGNRREELLAVCARLFREKGFDGTSIRDISEAAG
                     MHSGSPFYHFKSKQEMLVAVMEQGLTEGLRHLEAVALRKLPPADKFRQLVRTHLETIC
                     GDGHDFIPVMLYDWRSLTPANRRRVIALKDRYDALWQKVTDELQAQGVMPGDAQLARL
                     FTFGVLNWTARWYRPGGRKSLDEIAEEAARFLLRG"
     gene            complement(24530..24682)
                     /locus_tag="AC16O9_11825"
                     /pseudo
     CDS             complement(24530..24682)
                     /locus_tag="AC16O9_11825"
                     /inference="COORDINATES: protein motif:HMM:NF036723.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24989..25168
                     /locus_tag="AC16O9_11830"
     CDS             24989..25168
                     /locus_tag="AC16O9_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517342.1"
                     /translation="MNNPYLDDASKNVREWAPRDMFGADAMSFTQTKHLGSNRARLSQ
                     IINGKWTPVTDHITE"
     gene            25245..25847
                     /locus_tag="AC16O9_11835"
     CDS             25245..25847
                     /locus_tag="AC16O9_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR6517343.1"
                     /translation="MLSTAGGIGVFFYAYFWAMRHPLGAVTVMPVTWVDELIGFQPLS
                     FPLYVSLWIYISLGTALAKDLRELATFGAASLAMSVVGLAIFMLLPTRAPDFAIDWSL
                     YPSMQFLKEVDVAGNAFPSLHAAFCVFTAAVLHAQLSTLGAGRWLRAANLLLCAGILY
                     STMATRQHVALDVIAGAVLGGVASAAYLGALGERKRAQGG"
     gene            25849..26238
                     /locus_tag="AC16O9_11840"
     CDS             25849..26238
                     /locus_tag="AC16O9_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR6517344.1"
                     /translation="MALRVVRLGTRRAADEGPRLGTVRRPPRGVPRARFAKDDWYDLW
                     LPDLAPSEPLVKQALRAQSNAQWRAFAKRYRKEMAAPEKTRLLELLAALSKVANFSVG
                     CYCEDEARCHRSVLRRLLRERGAKLVS"
     gene            complement(26207..>26527)
                     /locus_tag="AC16O9_11845"
     CDS             complement(26207..>26527)
                     /locus_tag="AC16O9_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517345.1"
                     /translation="FEDVLRDEPHHASGIAGALRARPAPAAQMRRLIDRLSTHPSCAV
                     RTAALSLEGSVDAAQAALRSSCWRLQARALHILEERGVAPLDLSAVPLFLRPATTRAS
                     RRAP"
BASE COUNT         4289 a         8852 c         9232 g         4154 t
ORIGIN      
        1 gcctgcgcgg cctgaaggaa aacgtgatcg tcgggcggct cattcccgcc ggcacgggca
       61 tggcgtacca caagatccgc aaggatcagg ctgccgagcc gccgctgctg gagatcccgg
      121 cggcggagga gacgaccccc gccaccgagg tgacgacggc gagcggcggc tagcgctaga
      181 cccggtacaa ggcgcgctgg tcgcggcgga aagcgatcgg ctgcgccttc gggtccgaca
      241 ggggccggcc caggctgatg cggccgctgc cgtgcagcac ggtctcgccg cgcagtacgc
      301 gcacctcgcc cggcgtgccg tcgagcgcca cgtaggttcc gttcgcgctg cgatcggtga
      361 acttgaagcg catcgtgtcc acctccacca ccgcgtggct gcggctggca tagtcgtccg
      421 cgaccacgat atcgttgcct gcgtcgcgac cgaaggacag gcgcggattg aaggcattca
      481 gccggtgcat cgcagcgccg aaaaggaact gcagggcgcc ttcgtcggcg ggaagtacgc
      541 cgaccgcggt cgagttcacg ttggtgacgc cggcgcggtc gggctgccag acgacctcat
      601 gcaccaggga ttcgcggtcg tcgcccttca ggcgtgcggc gtaggcggca cgcgcggtgg
      661 cgcggatcgg gggcgacagc tgctccaccg ccgcctgcgt agcgaggatc tggttggcgc
      721 gtgcgatggc ggccaggtag gcggcgacat tgaccacggt gccgaagacg tctgcgccct
      781 cgacgatcac cgatccccag ctgatgcccg aatgcacgcg cagcggctgc tccggagcgc
      841 gcaccgcagc catgcccgcg tggagcgcgc ccatcgccag cacggcccgg tcggcgtccg
      901 gaaaggcgca catgacgccg tcgcccaggg tcttgacgat gcgtccctgg tgctgcggca
      961 gcaccgccgc caccctgccg tagaacacct gcgtcaggtc gcgcgccgcg gcgtcgccga
     1021 gctcgcggaa tatgcgggtg ctttccgcga gatcggcaaa gacgatggcg tagtcgcggg
     1081 tttctttcaa ggtggactcg gcgcggcagc cggcatcata caggcggcgc gcgagtcgct
     1141 ctgtgaacgg atacgcatat aattaccccc ctcatgggca agctcgagcc gcttcgagca
     1201 cgtacggccg ccgagcgcca ggcgcgcgtc gatctggcag cggctttccg cctcgccgtt
     1261 cgcatgacct gcacaagcgc agggccgcgt gcgttcggcg cgcccacctg ccgtacgcca
     1321 cgaggctgac gatgcttgag ccagggcgcc tcgagatgat ggagcggaac gcgctccagc
     1381 gtctccttga ccgcgaagag cccgagtacg cgcgttgacg ctagcgtgcg cgacgctgcg
     1441 ccgggcgatc tgccggcggt gcaggccatc tacgcacagc acgtgctgcg cgggctcgcc
     1501 tccttcgagg aggtcccgcc cgacctcgag gaaatgcgcc ggcgccatgc ggacgtgacc
     1561 ggccgcggct tgccctacct ggtcgcggcg cgccagggcg aggtgctcgg ctttggttac
     1621 tgcgcgccgt accgcgcgcg ctccgcctac cgctacgcgc tcgaagactc ggtctatatc
     1681 agggaaggca gcgccggcca gggcctgggc tcgagaatcc tcgccgagct catcgcgcgg
     1741 tgcgagcggc tcggctaccg ccaggtcatc gcagtgattg gcgacagcgc caacgccgcc
     1801 tccattgcgc tgcacgcccg gttcgggttc ctgcgtgtcg gcacgctgcg ctccaccggg
     1861 tacaagttcg gccgctgggt ggacagcgtg ctcatgcaga ggccgctcgg ccccggggac
     1921 ggcacgccgc ccgctacttg aatagcagcg cgatgccgct gccggcgagg agcactgcca
     1981 ccacgcgctc gaactttgcc tggtcgaggc gcgcgtgcca acgatcaccc accaccatgg
     2041 cccccaccat gaccggcgca aggagcgcga gcgccgcgat cgcacgccgg tcgaagaatc
     2101 ccaaggtgcc gtaaccgccg gctcggacca acccgaggca cagcagtacc gaggacatgc
     2161 tggcgcgaaa gcggcgcttg tcgagcgcga gcgcgcgcag gtagatgacg tagaaggggc
     2221 ccgccatgcc gccgaacagc gtcgctacca gccctcccga gcctccggcg acggcggcaa
     2281 gtgccggcgc tggcagccgc cacaccgggc ggcgcggcag caggccccag gaaccgtaaa
     2341 ggatgatgaa cagcgcaaat gccttgacga gcagcgcctg gtcgagcgag gcgaacagcc
     2401 acaatcccag tcccagcccg acggccgacg gcagcgcgag gccgcgcagc gcgcgccagt
     2461 cgacgaagcg gaattcctgc gccgactgcc cgagcgaagc gacgatgccg agcgtcgtga
     2521 tcaccgggat cacgaccgtg agcggcatga acagcgcgag cagcggaatc gccaccacgc
     2581 cggccccgaa gccggtcgcg ccgcgcagcg tgtaggcggc gaacaccacc gcgacgcaga
     2641 aagcgagcgc cggcgccgaa gccaggctcg tcagcgcatc gatcaagcgc cggcccgcgc
     2701 aagaaccccg ccgtagaacg cgccgatggc gccggcgccg ccgacgcggt tgctaggcac
     2761 cgatggtgac gacgactttg cccttggctt tgcgcgaggc gagcaggttc atggcctcgg
     2821 cggccttctc cagcggatag cgcgctgaga catggggcct caacctgccc tcccggtacc
     2881 agcgcgaaag ctggcgcgcg ctttcggcga acacctgcgg ctcgcgccgc gtgaagtcgc
     2941 cccagaacac gcccaccacc gacgcgccct tgagcaacgg gaggttgagc ggcagctttg
     3001 ggatgtcgcc ggcggcgaac ccgatcacca ggtgccggcc gcgccaggcg atggaacgga
     3061 acgcgggctc ggtgtaaggg ccgcccaccg cgtcgtagat cacgtccacg cccttgcctg
     3121 cggtgagcgc cttgatgcgc tcgcgcagat cctcggtggc gtagttgatg gtctcgtcgg
     3181 cgccgtgctc gcggcacaat gcgagcttgt cgtcgctcga agcgcaggcg atgacgcgcg
     3241 cgtccatcgc tttgccgatt tcgagcgcgg cgacgccgac gccgcccgcc gcacccagca
     3301 ccagcaggga ttcccctgcc ttgagctgcc cgcggtcgcg cagcgcatgg tcggtggtgc
     3361 cgtaggtgag gacgaaggac gcggcctcgt catagctcat gccctcgggc atgggtagca
     3421 ggcgcccagc attgacattc acctcttcgg caaaggcgcc gaaggtggtg taggcgatca
     3481 ccttgtcgcc cggcttggcg ttcttcactc cggcgccgac ctccttgacg acgccggcga
     3541 gctcgctgcc cggcgaaaag ggcagcggcg gcctgaattg gtattttccc tggatgatga
     3601 gcacgtccgg gaagttgacg ctcgccgccc ggacgccgat caccacctcg tccgcgcccg
     3661 gcctgggcga gggcacgtcc tcgagcacca gggactcggg cggaccgaac tgtttgcaga
     3721 gcagcgcttt caagcggacc tccttcagcg gctacgagga gcgcgcggcg aggccgccgt
     3781 caatggtgac gatgcagccc gaggtgtagg cggaacgctc ggaggcgagg aacgccgcca
     3841 tccaggcgat ttcctccggc gtggccggcc ggccaaagga catgccggcg aagagctcgg
     3901 catagcgctc cgggtcgccg aacttcgcct ccgcacgctg gcgcaacagg ctcttcaatc
     3961 ggtcggtggc caccggcccc ggattgatgc cgacgacacg gatgccgtcg gcgtgcgaag
     4021 cgccgccgag cgtccgggta aaggccatga ggccggcgtt ggccgtgctg ccggcgatgt
     4081 agctcgcgtc cagcctctcg ccggcgttgc ccaggatatt gacgatcacg ccggacttgc
     4141 gggacttcat gcgcgcgtac acctggcgcg tcaagttgat gtagccgaac accttcaagt
     4201 cccaagcgcg gcgccaggtc tcctcgtcga ccgtgagcag atcgccgccc gggatggcgc
     4261 cggcgttgtt gaccaggatg tcgagcccgc ccgcccactc ggccagctgc tcggcggcgc
     4321 cgcgctgcga cagatcggtg gcgcgaaact tgtgccggcg cgccgcgggc gggttgcgcg
     4381 ccgcgatcat cacctcgcag ccctcgtcgg cgagcgcgtt ggcgcaggcc cagccgatgc
     4441 ccttcgaacc gcccgttacc agcgcgcgtt tgcccttaag attcaggtcc atcgccacag
     4501 agtctaccaa catgcccacg attacgaccg acgacggcgt gaaactgcac tacgaggagg
     4561 cggggcgggg gacgcccgtc gtcttcgtgc acgagttcgc cggcgacctg cgcagctggg
     4621 agccgcagtt cgcccatttc tcgcgccgct accgctgcat cgcctacaac gcgcgcggct
     4681 acccgccctc ggacgtgccg gaggatttcg agcgctactc gcaggaccgg gcgcgcgacg
     4741 acgtgcgcgc cgtgatggac gccctgaaga tcgacaaggc ctacgtggtc ggcctgtcga
     4801 tgggcgggtt cgcgtcgctg cacttcggca tcaagtacag cgcgcgcgcg ctggggatcg
     4861 tggtcggcgg ctgcggctac ggctcggtca aggcaacgcg cgatgcgttc caggaggact
     4921 cgcgcgccaa cgcgcggcag atgatgtccg aggggatgga gaggttcgcc gcaacgtatg
     4981 gccatcgtcc cacccgtatg cagctcaagg cgaaggaccc gaggggattc gacctgttca
     5041 tccggtactt ggaggagcac tccgcgctcg gctcggcgaa taccatgctc ggctaccagg
     5101 cgcggcggcc gtcgctgtac gacctggtcg acgagatgaa ggcgattacc gcgcccacgc
     5161 tgatcatgac gggggacgag gacgacgggg cgctggagcc gagcgtgctc atgaagcgca
     5221 cgatcccgca cgcggggctg gtggtgctgc cgcgcaccgg ccatattctc aacctcgagg
     5281 acccggcgct ttacaaccgc atcgtcgagg atttcttcca ccaggttgaa agcgggcgct
     5341 gggcatgagc gcgggaaggc tcgtcgtcga aaagcgcggc gccgtgggct ggatcgtgtt
     5401 cgaccagccg gcgaaaaaga acgccatcaa cgacgccatg tggcgcggca ttcccgaagc
     5461 gatggcgcgc ttcgatgccg acgcggacgt gcgctgcgtc gcgttccgcg gcgcaggcac
     5521 cgaggcgttc gcggcggggg cggacatctc ggagttcgag aagatccgcg cgcatcgcgc
     5581 cgcggtcggc gaatacgacg ggctgctcga tcgcgtgctg ctctcgatcc agggctcgct
     5641 caagccctcg gtggcgctga tccacggctt ttgcatgggc ggcgggctcg aggtggcgct
     5701 cgcctgcgac ctgcgctatt gcggcgcttc ggcgcagttt gggattcccg ccgccaagct
     5761 cggcctcgcc tacaacgtcg aaggccacaa gcgcctgctg gagacggtgg gacacgcccg
     5821 tgcgcgggaa atcatgtttc tcggccggcg ctaccccgcc gccgaggcgc tggcgatggg
     5881 gctggtgcac cgcgtgctgc cggatgcgga gctcgagggg cacgtcgacg gcgtgatcgc
     5941 gacgctcatc gcgaacgcgc cgctcagcat cgccaactcc aagaccattc tcgaggagta
     6001 cgcgaagtcc gtcggcttgg cggatcacca gcgcatgcag gaggcgatgg atcgctgcac
     6061 gaagagcgcg gattacgagg aggggcgacg cgccttcatg gagaagcgca agcccgcatt
     6121 caaggggcgc tgagatgaag atcgagtcga tccgcacgcg cgccgtcgcg gcgccgatga
     6181 agcggccgct cgccaccagc accggcgcgc tgacggcctc gtcgctcctg cttctcgatc
     6241 tgcagaccga tgctggcccg gtcgggcgct cgtacctttt cggcatcggc gcgcacaacc
     6301 tgaagccgat cgcggcactg gtcgaggcga tggccgagat gctgaagggc gacgcggtcg
     6361 cgcccgtcga catcgagaag aagctgcgcc agcgctacac gctgctcggc gtgcacaaca
     6421 tcgtgctgtt cgccatgtcg ggcatcgaca tggcggcttg ggacgcgctc ggtcaggcgc
     6481 aggggctgcc gctggtgcgc atgctgggcg gcacgccgcg gcccatcccc gcgtacaact
     6541 ccaagggcct cggcatcatg ccgctcgagc cgcttgccaa ggaggccgtc gagctggtca
     6601 aggagggttt cgccgcggtg aagctgcgcc tggggcgccc gcatgccgcg gacgatctcg
     6661 ccgcgctgcg tgcggtgaaa aaggcgatcg gcaccgacat cacgctgatg gtcgacttca
     6721 accagggcct gaccgtcgcc gaagcggtga agcgcgggcg catgatcgat gacgaaggcg
     6781 gcgtgacttg gatcgaggag ccggtacgcg ccgacgattt cgccggcaac gcgcgcatcg
     6841 cgcgcgaggt ggccaccccg atccagatcg gcgagaactt catgggcccg gagcagatgg
     6901 cgcaggcgct cgccgccggg gcctgcgact acgtcatgcc tgacgcgcag cgcatcggcg
     6961 gcgtcaccgg ctggatgcgc gcggcggcac tcgcgcaggc ggccggcatg gagatgtcga
     7021 gccacctgtt cccggaagcg agctgccacc tgctggcggt gacgccgacc tgccactggc
     7081 tcgagtacgt ggactgggcc gacgcgatcc tcgaggaccc ggtgcaggtg aagaacggcc
     7141 atgcgctgat cccggagcgc ccgggcctgg gcatgcgctg ggacgaaaag gcggtaacga
     7201 agtaccagct ctgatgttca cgcgacactt tgcgcactgg ccgccaggcg tgccgaagac
     7261 gctttcggtg ccgcgcacca gcctgttctt caacctcgag gtgagtgcgc ggcgctatcc
     7321 cgccaaggca gcgctgcact actacggcac cgacgtgtcc tacgcggagc tgctgcgctc
     7381 ggccgaggcg ctggccgggt tcctgcagca gcgctgcggc gtgcagcgcg gcgaccgggt
     7441 gctgctctac gcccagaact gcccgcaatt cgtgatcggc tactacgcca tcctgcgcgc
     7501 ggacgctgtc gtggtgccgg tcaacccgat gaacaggcac gacgagctcg cgttctacat
     7561 ctccgattcc gacgcgacgg tgattctgag cacccaggac ctggccgccg agatcgagcc
     7621 gcacctgggc caggggctca ggcactgcgt gctgggggcg tactcggact acctgcgcga
     7681 gccgaccgac ctcggcgtgc cggaggcggt gcgcgcgccg cgcagcgccg cggccacatg
     7741 ggtcgaggcg ctcgcggccg gctacgcgcc cggcccgcac accgccggcc cggatgatct
     7801 cgcggtgctg ccctatacct ccggcaccac gggacacccc aagggtgcga tgcttctgca
     7861 ctcgaacctc ctcgccacca gcactggcgc ctgtgcctgg atccagtcct cgagcgaggc
     7921 ggtggtgctg ggcgtgctgc cgatgtttca cctcaccggc atgcaggcca acatgaacgt
     7981 gccaatccag ctcggcgcca cctcggtgct gatgacgcgc tgggaccgca gcgtggcggc
     8041 ggaattgatc caacgccgtc gcgtcacgag cgtcaccggc atcaccacca tgatggtgga
     8101 tttcctcgcc aacccggagc tcggaaggta cgacctgtcg tcgctggtgc gcgtcggcgg
     8161 cggcggcgcg gcgatgcccg aggcggtggc ggcaaagctg caggagcgca tcgggttgcc
     8221 cttcatcgag ggctacggcc tcaccgagac cgcggccccg tcgcacgcca atccgccgca
     8281 ccgcccgaag cgtcagtgcg ccgggatccc gtacttcgac acggattcgc gggtcatcga
     8341 tccggtgagc ttgagggagc tcgggccgaa ggaggtcggc gagatcgtca cgcatgggcc
     8401 gcaggtgttc cagggctact ggaagcagcc gaaggccacg gccgacgcct tcatcgagca
     8461 cgatggcaag aagttctttc gcaccggcga cctcggctat tacgacgagg acggctactt
     8521 cttcatcacc gatcggctga agcgcatgat caacgcctcg ggcttcaagg tctggccggc
     8581 ggaagtcgag gcgatgctgt acgcgcatcc cgacattcag gaggcgtgcg tcatcggcac
     8641 gcgggatgcg catcgcggcg agacggtgaa agcgatcgtg gtgctcaagc ccgccgcgcg
     8701 cggcaagttg cggcccgagg acatctcgga ctgggccaag gcgcacatgg cggcgttcaa
     8761 gtacccgcgc gtggtggaat tcgtcgagca gctgccgaaa accggcaccg gcaagatatt
     8821 ctggagaaga ctgcaggaag aggagaacaa gcgatgcggt gcgggttagc tcttctgctg
     8881 ctgacggcac tgccggcctg ggcccaggtc acggccgagc agccctggag ccgtgccacg
     8941 ccgccagggg cgaatgtcgg cgtcggcttc ctgcggctga agaatgccgg cgccgcggcg
     9001 gagcgcgtgg tcggggcgtc ctcgcccgtg gcggggcgcg tggagatgca cgtcacgacg
     9061 cgcgagggcg acgtcatgaa gatgcgccag gtgcaatcct tcgagatccc ggcgggcggc
     9121 agcttcgagc tcaggccggg cggcgcgcac ctgatgctca tgggcttgca gcggccgctc
     9181 gcgcagggcg agcgcgtacc gctcacgctg aagctcgaga acggcggcac gctgcaggtc
     9241 gagcttgccg tcgagccgct gggcgcacgc cagccggcgc accggcagta agagagcagc
     9301 cggccgagct atcgatactc cccgatgggc tcaactcggg aagaggtcat ggttgcgact
     9361 cgcgtctttc agcgctgcgc ctacgcgctt tcgtaaagcc tcgtcatcac gaactcgcga
     9421 tgcccgagcg cttcggccgc ggtcaggcgt ccgttggcgg tgcggaacat gcactcgagc
     9481 agcttgtcgc ccgcctggtc cggggtcagc tccttcctca gcagccccga gacgtcgacg
     9541 tcaatgtgct cgctcatcgt gcgcacagtg cgcggattcg ccgagagctt gatcaccggc
     9601 aggatcgggt tgccgatgac gttgccctgg cccgtcggga acaggtgcac cgtgaagccc
     9661 gccgccgcgc aaagcgtcac catctcggcc gccgccgacg aggaatccat gaaccacagg
     9721 cccggcccgg tgggcatctc ggccttgtcg agcacgccgt ccaccttgca caggcggccg
     9781 atcttctgga tgttgcccag ggccttttcc tcgatggtgg tcaggccgcc ggcaatgttg
     9841 cccttggtcg gctgcgagtc cgacaggtcg ctcgtcttgt ggcgctcgat cacttcctgg
     9901 tagcggtcga acaccgtctg gaatttcgtc ctcactgcgt cattccggca gcgcgcggcc
     9961 accaggtgct cgccgcccgt cagctccgtg gtttccccga acaccatcgt gacgccgcgc
    10021 gggtagagct tatcgaaggc gttgcccacc gtcgggttcg ccccgcagcc cgaggtggtg
    10081 tccgattcgc cgcacttggt ggagacccac aggtccttca gcgggcgctc ctcgcgccgt
    10141 agctccgagg cccattgcac gtactccttg gcggccttcg atgcacgcat gatcgtgtcg
    10201 tggtcgccgt gctgctcgat gccgaagtac gacacgggct tgccggtcgc ggcgatgccg
    10261 tcggcgacct tcttcgccca gccgtcttcg atgcagatca ccaccaccgc ggcgacgttg
    10321 gggttggagc cggcgccaat cagcgttcga aagtgcagct cgaggtcggc gccgaactgc
    10381 agccggccgt agggatgggg gatcgccagc gtgcccttca cgttgttctc cacggcctgc
    10441 gccgccgcgt tggacaggtc gtccaccggc aggatgagga cgtggttgcg cacgccgatg
    10501 cgctgattct cgcgcctgta gccccagaag ctcgctttgg aaaggtccat ggtcaccacc
    10561 gcttggtctt catgtctaca ttttagctct ggcgatcaga gcggggtccc ggcgaccggc
    10621 gccggctcta gaacagcagc cggccgacgc cccaggcgat cgccgcaatg aaggccgagc
    10681 aggggatcgt caggatccag gcccagacga tgttgcccgc cacgccccag cgcaccgccg
    10741 atacccggtt cgccgagccc acgccgacga tcgagcccgt gatggtgtgc gtggtggaga
    10801 ccgggatgcc caggctggtg gcgacgaaca gcatcatcgc gccgccggtc tcggcgcaga
    10861 agccgcccac cggcttgagc ttggtgatgc gctgacccat ggtcttgacg atacgccagc
    10921 cgccgaaggc ggtgcccgcg gcgatcgtca cgtagcaggc gatcaccacc cagacgggcg
    10981 gcgaatcggc accgctcagt ccggcggcga tcagcagcat ccagatgatg ccgatggtct
    11041 tttgcgcgtc gttgccgccg tgccccaggc tgtagagcga ggccgacacg agctgcaggc
    11101 gccggaacca gcgatccacg cgcgagggcg aacttcgcac gaacagccac gcgacgagaa
    11161 gcatgagcag tgcgccgagg acgaagccca tcagcggcgc gatgaggatg aacgccacgg
    11221 tcttgagtat gcccgccggc agcagcgaca cggtgcccgc cttggcgacc gcggcgccca
    11281 ccaggccgcc gatcagcgcg tgcgaggacg acgaggggat gccgtaccac cacgtgatca
    11341 ggttccagct gatcgcgccg atcagggcgc cgaacaccag gtagtgatcg accacgccct
    11401 ggtcgacgat gcccttgccc acggtggtcg cgaccttgag ctggaacacg aacagggcga
    11461 ggaagttgaa ggcggcggcg agcatcaccg cctggtaggg cttgagcacg cgcgtcgaaa
    11521 ccacggtggc gatcgagttg gcggcgtcgt ggaagccgtt catgaagtcg aacaccagcg
    11581 ccaccagcac cagcgcgatg acgacgccga gcgcgatttg cacgggggcg agcgagtcca
    11641 tggcgtggct caggagttct cgaggacgat gccctcgacg atattggcga cgtcctcgca
    11701 gcggtcggtg atcgactcga gcaggtcgta gatgacccgc atcttgatca cctgcttcag
    11761 gtcgttctcc tcgcggaaca gcttcgagag cgccgagcgc agcacccggt cggcgtccga
    11821 ctcgagccgg tcgatctcct cgcagatctt gaagatcgcc tcggtgcgct tgacgttggt
    11881 gagcagcttg acgaccgacc cgacccgctc gcagctcatc tggcagatgc cggcgagctg
    11941 cttggcctcc ggcgtgatgc gccgcaggtc gtacaggctg accgactcgg cgacgtcctg
    12001 gatcaggtcc aggatgtcgt ccatgcgcgt gatcagctgg tggatatgct cgcggtcgaa
    12061 cggcgtgatg aacgtccggt gcagcatcgc gatcgtctcg tgggtgatct tgtcggcggc
    12121 gcgctcggcc gtgtcgatgc gctgcgtgta gccctcgagg tcgttgaagt tctccatcat
    12181 gcccgcgagc tcgcggctgc cctcgacgat gcgctcggcg tgcgcattga aatgatcgaa
    12241 gaagcgcccc tcgcgaggca ttagacgtga cagcatgtct cctcccttgg cgaacccgcg
    12301 ggcgcgcccg tcagtcgcga aaacccaggc cgagcgatct cagcgtctcg atctgcggcc
    12361 cgtcgaagcc ccactcggcg agcgcttcgc gcccgccctc gccgcgctcg ggcggcgcgc
    12421 ggcgtaccgt ggcgcgcgtg cgcgagaagc gcggtgcggg cgccggctgc tcgaccttgg
    12481 cgagctcgat gaagcttccg cgcgccacgt tgtgcgggtg gcgcttggcc tcgctgaagc
    12541 tgagcaccgg cgcgaagcac gcatccgagc cctcgaaagc ctgtgcccac tcatcgcgcg
    12601 tcttcgcggc gatcgcccgg gcgaagcgct cgcgcagctc gggccagcgc tcgcgctcgt
    12661 gctggccggg cagcgtctgc ccggaaaggc cgagccgctc gatcagctcc tcgtagaagc
    12721 gcgcctcgat ggcgccgatc gcgacgtact tgccgtcccg ggtcgggtac acgtcgtacc
    12781 agggcgcgcc gccgtcgagg aagttctcgc cgcgcgcctc gctccagcgc ttcgcggcga
    12841 gcattccgga aaacatcgcc gccagcagcg aagcgccctc gaccatcgcc gcgtcgacca
    12901 cctggccctt cccggagcgg cccgcttcga ccagcgcgca ggcgacgccg aagccgagca
    12961 gcatgccgcc gccgccgaag tcgccgacca ggttgagcgg cggcaccggg gcgtcgccct
    13021 tgcggccgaa ggcgttcagc acgccggcga gcgcgatgta attgatgtcg tgtccggcgc
    13081 gcggcgcgag cgggccgtcc tggccccagc cggtcatgcg cccgtagacg agcttcgggt
    13141 tgcgcttgag ggccgcctgc ggccctaggc cgaggcgctc catgacgccg gggcgaaagc
    13201 cttcgatcag cgcatcggcg cgcgcgacga gctcgagcgc cgcctcggca cccttgggcg
    13261 acttgagatc gagcgtcacc gagcgccggc cacgcatcat gacgtcgttc cagcgctcgc
    13321 gcttgaggcc gaggtcggcc tcggccgcgc ggtcgaccag cagcacgtca gcgcccatgt
    13381 cggcgaggag catccccgcg aacggccccg ggccgatggc ctcgaactcc aggaccctga
    13441 tgccggcgag cggtcccggc acggggcgct cagtgaattc cgagggcggc ttcgatgttg
    13501 cgcaccatcg ccaccagccg cggcccgata tcctcctcga gctggccctt cttcagcaga
    13561 aacgccggca ccgcgcagtt gaacgcgacc acctcgccgt ccaccgtgcg ccgcatcggc
    13621 acgccgaccg cgtgcacatc gcgcagcagc tcgccgatgg agacgcagaa gccgcggctg
    13681 cggatgtcct ccatcgcctt gtcgatctgc ggccggttct tgcgatgcat ctcgggctct
    13741 ttcactttca tctggttgag cactgcctcg cgctcggggg gcgtgcacga cgcgatgaag
    13801 gcccggccga tcaccgattg ggcgatgggc accgcagtgc cgatgtcggg caggatggcg
    13861 aagccgttgc cgctgctgcg gcaggactcg acgtacacca tgctcatgcg atcgcggatg
    13921 cccatggaga cggcgccgct gcagtattcg gccagatcct tcatgaacgg acgggccacc
    13981 tggcgcacgc tcatcgaggc gagcagcggg tagccgatcg acagcactgc cgagccgagc
    14041 tggtatttgc cgaggcgcat gttgtggcgc aggtagccaa gcttggtgag cgtataggtc
    14101 agacgcgata ccgtgggctt gggcaggccg gtgcgcacgg agatttcctt gttgccgagc
    14161 atcggctcga gcggcgtaaa gcagcgcagg acctcgagac cgcgcgccag cgtcgtggca
    14221 aactgccggt cgccctcgtg ctcgtagctg tagtggcccg cggcggggga aagcggtgcg
    14281 tcgggctcga gtgcgcccat ggtgtctcct cttgttgtcg tcgttgcggc gggggccgca
    14341 aactgtgtgc tgtttcgata ttcgacccgt tggccgatca ctgtcaattt agtcatatca
    14401 tatttcgcat agcggaattc tgaaacaaat catggaccaa tccgccttcg atctgagcgg
    14461 caaggtggcg ctggtcaccg gctccacgcg cgggatcggc aagtcgattg ccgaggaaat
    14521 ggcgcgtgcc ggcgcgaaag tggtggtgtc cagccgcaag gccgaggcct gcgagcaggt
    14581 acgggccgag ttcgagaagc ggggctacga ggtgctggcg cggccctgca acgtatcgcg
    14641 caaggacgag ctgcaggcgc tggtggacgc ggcgctcaaa cgctggggac ggatcgacgt
    14701 cgccgtctca aacgcggcga gcaatccgta ttacggcccg ctcgccggca tccccgacga
    14761 ggcgctcgac aagatcttcc acaacaacgt gaagagcgtg ctgtggctcg ccgccctgac
    14821 gctgcccggc atggcggagc gcggcggcgg cagcttcatc agcgtgggct cgatcggcgg
    14881 catcgtcgcc agtaccgtga tcggcgccta cggcatgtcc aaggcggccg accacatgct
    14941 ggtgaagaac ctggcggccg aatggggacc gaagaacgtg cgcgtcaatg ccatcgcccc
    15001 gggcctgatc aagaccgact tcgccaaggc gttgtgggag gacgaaaagc gccgcaagga
    15061 gcgcgaggcg gccacgccgc tgcggcgcct cggcgagccc aaggacatcg gcgccatcgc
    15121 cgtgtttctc gcctcggagg cggcggcgtt catcaccggg cagaccatcg tcgccgacgg
    15181 cggcgtgacg atcctgtgac ccgatcgacg ctgcgctcgg ttctggccgc cggcgaattc
    15241 gtcgtcgcgc cgggcgtatt cgagatgctt tccgcgcgca tcgccgaccg cctgggcttc
    15301 aaggcgctct acatgaccgg ctacggcata tcggcgtcgc atctgggcct ggcggatgcg
    15361 ggcctggtca cttatcgcga catggtggag cgcgcgcgca ccattgcgca gggcacggcg
    15421 acgcccctga tcgcggacgc cgacacgggc ttcggcggcc tgctgaatgt gcgcgaaacc
    15481 gtgcgcggct acgaagcggc cggcgtgcag gcgatccaga tcgaagacca ggcgattcca
    15541 aagaagtgcg ggcacacgcc caaccggcgc gtggtgccgc tcgccgacgc ggtcaagcgc
    15601 atcgaggtgg cggtggccgc gcgcaagagc agcgacttcc tcatcgtggc gcgcaccgat
    15661 gcgcgcaccg gcctcggcct ggacgaggcg atccggcgcg cgcgcgcgtt cgcgcgagcg
    15721 ggcgccgaca tcctgttcgt cgaggcgccg gaatcggagg cggagttcgc gcgcgtcggc
    15781 gccgaacttg ccggcgaagc gaagctgctc gccaacatgg tgccgacggg ccgctcgccg
    15841 atgctctccg cggacaagct gcggaagttc ggcttctcca tcgccatcta cccgagcgcc
    15901 ggcatgagcg ctgcgtgcgc ggcgctggag ggcgcctatg ctcacctgct cgcgcgcggc
    15961 tcgctcgagg gctcccccgt ggccgcctac gacatgcagc ggatgcacga gttggtcggc
    16021 ttcgccgata tctgggaatt cgagcggcgc cacgtcgagc cgcaggactg acgcggtggc
    16081 agggcgcacc ctcttcgaca agctctggga cgagcacgtc gtcgaggatc tgggtgacgg
    16141 cgtgcagctg ctgcacgtcg accggcatct gctcaacgag ctctcgggcc atcgcggcca
    16201 gctggaaatc gcgaagcgcg gcctgtcgat gcgcaatccc gagctctcga tcgccacgct
    16261 cgatcacgtc gtgtccacgg cgccggggcg ccgcggcggc gacgcgccct gggccgagcg
    16321 catgatcgag gtctttcgcg ccgagacccg ccgcgccggc gtgccgctgc acgacttcgg
    16381 cgacgacggc cagggcatcg tccacgtcgt cggcccggag ctcggcttca cgctgccggg
    16441 ggcgctcctc gtgtgcggcg acagccacac ctgcacccac ggcgccctcg gcgcgctcgc
    16501 ctggggcatc ggtacgagcg agctcgcgca cgtgctcgct acgcagacga tcgtgcagca
    16561 aaagccgaag tcgatgcgcg tgcgctttga aggcgcgctc gcgcgcggcg tcggcgccaa
    16621 ggacatgatc ctcgccctga tcggcaggat cggcactgcg ggcggcgtgg gctacgccgt
    16681 ggagtacgcg ggcgcggcga tccgcgcgct cgaggtcgaa gggcgcatga cggtgtgcaa
    16741 cctctccatc gagctcggcg ccaagatcgg catggtcgcg cccgacgaaa ggacctacgc
    16801 ctatctcgaa gggcgccgct atgcgcccag gaacgggctc tggcgcgagg cgctccggca
    16861 ctggcgcacg ctgccgagcg acgaggaggc gacgttcgac ctcgagcacg agctcgactg
    16921 cgcggcgctc gcgccgcaga ttacctgggg caccagcccc gaggacgtga tcgcggtgga
    16981 cggcgtcgtc ccagacgcca agcctgcggc actggagtac atgggcctgg cggcgggccg
    17041 gccgatcgcc ggtacgcggg tcgaccgtgt gttcatcggc tcgtgcacca acgcgcgcct
    17101 gccggacctg cggctggccg ccgacctggt gcgcggccgc aaggcggccc cgcatgtgaa
    17161 ggcctgggtc gtgccgggct cgcagcgcgt gaagcgcgag gccgaagccg aagggctgca
    17221 cgaggtcttt ggcgctgccg gcttcgagtg gcgcgatccc ggctgctcga tgtgcgtggg
    17281 cgcaaacggc gacctggtcg agccgcgggc gcgctgcgtc tcgacctcca accgcaattt
    17341 cgtcggccgg caggggccgc aggcccgcac ccacctcgcg agcccggcga ctgccgcggc
    17401 ctgcgccatt gccggcgaaa tcgccgacgc gcggtgcttg tcatgacgcg cttcacgacg
    17461 ctgaccgcgc tcgcggcgcc tttcccgcag aagaacgtcg acaccgacac catcatccgc
    17521 atcgagcgct gcaccggcac gccgaaggcc gagctcggca agtacgcgtt cgagatggcc
    17581 cgctatctgc ccgatggcag cgacaatccg gacttcgtgc tcaaccagcc gcgctaccgc
    17641 ggcgcgcgca tcctggtctg cggcgaattc ttcggcaccg gcagctcgcg cgagatggcg
    17701 gtgtgggcgc tcgccggcct gggcatgcgc tgcctgatcg cgccgtcatt cggcgagatc
    17761 ttccgcggca actgcttcca gaacggcgtg ctgccgatcg ccctgccggc cgcggcggtc
    17821 gacgcgctga tgcgccgtgc cgaagcgccg ggcgcggcgc cgttcaccgt ggatctggag
    17881 cgccagacgg tgaacgggga gtaccgcttc gaggtcgacg cgcggcgcaa gaagatgctc
    17941 ctcgaagggc tggacgagat cgcgctgacg ctttctctcg agcctcgaat cgccgcctac
    18001 caggccgccg accgcgtgcg gcggccgtgg atctacgact tcacgcgctc ctgacgaact
    18061 cggcgcagcg ctcgccgatc atgatcgcgg gcgcgttggc attgccgccg atcagcgtcg
    18121 gcatgatcga ggcgtcggcc acgcgcaaat tctcgatgcc gcgcacgcgc aaatgcgcgt
    18181 ccacgacggc gtcctcgccc cggcccatgc ggcaggtccc ggcggggtga tagaccgtgt
    18241 ccgcgcgctg ccgcaccaga tcgaccaggg cctcgcccgt gatccagtcc tcgccgaaga
    18301 cgttcctgcc gcggaacggc gcgagcgcgg gctggcgcag gatctgcagc gtgagctcga
    18361 cgccgcgggc gagttgcgcc gcgtcgccct tctcagagag gaacgcgggg tcgatgagcg
    18421 gcggcgccag cggatcggcc gagcgcagcg cgacgcggcc gcggctcttc gggcgcagca
    18481 ggcatacgtg gcaggaaaag ccgtagcgcg gcgtcgtgtc gcgcccgtgg tcggcgacga
    18541 ggccgataca gaagtgcagc tgcaggtcgg ggcgctcgag ccccggcgcg gtgccgagaa
    18601 agccgccggc ttccgcgacg ttggaggcga gcaggccccg gccctcgcgc cggaaggcgc
    18661 caatgcctgc cgcgaggttc atcaggccgc gcgctgtcag gccgatcaac ccgggggccg
    18721 aggacaggta gccgatcacg tagtcggggt ggtcctggag gttctctccc accccgggca
    18781 gctcgtggcg cagcgcgatg ccgtggggct cgatggcgtg gcgcggcccg atgcccgaga
    18841 gcagcagcag ctgcggcgag ccgaaggcgc cgcaggcgac gatggtttcg cgccgggcgt
    18901 gcacggtgcc gcgcgaggtt tccacgccgc ggcaggcctt gccctccagc accagtcgca
    18961 gcgcatgggt gccggtgcgc agcacgaggt tgccgcgcgc cacagcggcg tcgacgaagg
    19021 cgcgcgccgc gctccagcgc cgcccgttgc gctgcgtcac ctggtacagg cccacgcccc
    19081 actgcgttgc gccgttgaaa tccgcgttgc gtgcatggcc ggcttcgacg cccgcctcga
    19141 cgaaggcgag cgacaccggg ttgggcgagc gcaggtcggc cacgttcaag ggcccgccgg
    19201 tcgcatggaa gtcgtcgctg ccgcgctcgt tgtgctcggc gcgcaggaac gcgggcagca
    19261 cgtcgcgcca cccccagccc ggattgcccg ctgcggccca gtcatcgtag tcgcgcggat
    19321 gaccgcgcac gtagatcatg gcgttgatgg cgctcgagcc gccgagcgcc ttgccgcgcg
    19381 gctggtagcc gcgccgtccg gcgagcccgg actggggctc cgtttcaaac gcccagttgt
    19441 gcagccgccg cggcaccgtc agggcaagca gcgccggcgt atcgatgagc aacgaacgtc
    19501 cggcgccgcc cgcttcgatc aggcagacgc gcgatgcggg atcttcagca agccgcgcgg
    19561 cgagcgcgca gcccgcggag ccgccgccga cgacgacgta gtcgtactcg ttcatgcgca
    19621 gtcaaactag gggtattctt tcgctctggc aagaggaggc gcagtcatgg acctgaacta
    19681 ctcccaggaa gagctcgcgt tccgcgacca ggtgcgcggc tggctgaagc agaacctgcc
    19741 cgccgacctg cgcgacaaga tcgccgccta cgaggagttg agccgcgacg acctgctgcg
    19801 ctggcacaag atcctcgccg cgcagggctg ggtggcgccg tcctggccga aggagtgggg
    19861 cggcaccggc tggagcgtgg tgcagcgcta catcttcgag gaggagtgcg gctatgcggg
    19921 cacgccgccg ctcatcccct tcgggctgtc gatgtgcggg cccgtgctgc tgaaattcgg
    19981 caccgaggag cagaagaaga cgttcctgcc gcgcatctac aacggtgacg atttctggtg
    20041 ccagggctac tcggagccgg gctcgggctc ggacctcgcg tcgctcaaga ccaaggcggc
    20101 gagaagcggc gagcattacg tcgtcaacgg ccagaagatc tggaccacgc tcgggcacta
    20161 cgcggactgg atcttctgcc tggtgcgcac cgattccagc gcggccaagc gccaggaagg
    20221 catctcgttc ctgctcatcg acatgaagtc gcccggcatc accgtgcgcc cgctgatcct
    20281 gatggacggc ggccacgagg tgaacgaagt gttcttcgag gacgtgaagg tgccggttgc
    20341 gaacctggtg cacaaggagg gcgagggctg gacggtcgcc aagtacctcc tcggccacga
    20401 gcgcatgaac accggccgca tcggcgcctc caagcgcgag ctggcaaagc tgaaggagtt
    20461 cgccgcacag cagaaaaagg gcggccggtc gctgctggaa gacacgcgct ttcgcgacag
    20521 gctctcgcgc ctggaagtgg agctgatggc gctcgagatc accaacctga ggttcctgga
    20581 ccgcatgcgc cgcaccggcc agccgccggg cgcggacgtg tcgatgctga agatcaaggg
    20641 caccgagatc cagcaggggc tcaccgagct catgatgcag gcggtcgggc cgctcgcgca
    20701 gccgttcaag gcggtgaacg gcgatgcgga tttcgatgcg ttcaccgcgg cgatcgcgcc
    20761 gcgctactgc aatttcagga agacgacgat ctacgccggg tcgaacgaga tccagcgcaa
    20821 catcatcgcc aagatgaccc tggggctata gccatgaact tcgactacaa cgaagagcag
    20881 cagctgctcg ccgattcggt ccggcgtttc atccagaagg actacgagtt cgaggcgcgg
    20941 cgcaagatcg tcgattcggc gcagggcacc agcgagaagg tctggggcac gttggccgag
    21001 atggggctca ccggaatccc gttctcgccc gactacggcg gcttcggcgg cggcgccatc
    21061 gacctgatga gcgtgatgga ggccttcggc gaggggctgg tggtcgagcc gtacctcgcc
    21121 acgctcctcg gcgcgcagtg tgtggcgcgc gccggcagcg acgcgcaaaa gaaggcgctc
    21181 ctgccgcagg tggtggaggg caagctcaag atggcgttcg ctttcagcga gaagggcgcg
    21241 cgctacgacc ttgcgcccgc agcggtgacg gcgaggaagg caggcagcgg ctggacgctg
    21301 gagggcgaga aagccgtcgt gctcggtgcg ccggcggccg gaaagatcgt ggtgtcggcc
    21361 aaggcgccgg cgggcccgag cctgttcctg gtcgacgcca agggcgtgaa gctcaagccc
    21421 taccgcacgc tcgacgcgat gcgcgccgcg gacgtcgcgc tgtccggggc ggcggcggag
    21481 ctgctcggcg ccgaaggcaa ggcgctcgcc gtgatcgagg aggtgatcga cttcggcact
    21541 gcgctggtgt gcgccgaggc ggtcggggcg atgaagttcg cctgcgacac gacgctcgag
    21601 tacctgaaga ccagaaagca gttcggcgtg ccgatcggca ccttccaggc actgcagcac
    21661 cgcatggtgg acatgttcgt cgcctacgag caagcgaagt cgatggcctg cctcgcgtgc
    21721 tcgaaggtgg acggcgcggt tgacgcgaag gagcgcgcgc acgcggtgtc ggcggcgaag
    21781 atcaagatcg ccgacaccgc gcggctcatc agccaggagg cgatccagct gcacggcggg
    21841 atgggcatga gcgaggagct caaggtgagc cactcgttcc gccggctcac catggtggcg
    21901 cagcagttcg gcgacgccga ccatcacctc gagcgcttcg ccgcgcaggg ctaggcggcg
    21961 gcgacttccg cgggtgtcga acggcgcctg gggctcacca tggcaagccg aaccgttgtc
    22021 attgcgggcg ccacggggct cgtaggcagg gagatcctgg cggggctgct ggcggatcct
    22081 tccgtttccg ccgtgcacag cctcgggcgc cggaagctcg gtgttgagca tcccaagctg
    22141 acttctcacg tcgtcgactt tggtgcgctg cctgccttgc ctgctgcgga tgaggtttac
    22201 ttggcactgg ggactacgat caaggtcgcc gggagccagg cggcttttcg cgccgtggat
    22261 tacgacgcca acctcgcggt ggcgaaggcg gcgctggccg caggcgtgac gaaggtcggg
    22321 ctggtgagct cgatgggggc ggattcgaag tctggaattt tctacagccg ggtgaagggc
    22381 gagctcgagg acgcgctgtc ggagatgccg tttgcggggc tagttatcgc acggccgtcg
    22441 atgctgctcg ggaatcggga ggagcttggg cagcctgtca ggcggggcga ggaggtggcg
    22501 tccgttctgg cgaagatagg aggcttcctc attccggcga actacaagcc gatcgcggca
    22561 tcggccgtcg caaaagcatt gctatctcgt gttccgtccg ccagcggtaa agcggtgctg
    22621 ctctccggtt ctatgcgctg acgtcacgta cggcatcgct ccttagtcag cctgcctacg
    22681 agaactctgc tcgaactctg ctttcctgcc gcgcgtagcg cgaagtgaaa ggcgtaggtc
    22741 aacaacgcgc ttttaggtgc tacgcgtcct ccacctgaac gtagaccgaa cgaagcctca
    22801 cgtgtactct gggaaattgt tctataccga cacgcactgg aggggaattt gaagaagttc
    22861 atgggtttac tgctggcggt gttcatacat gctctcttct ttcaaacagc cttagcacag
    22921 gacgggaaaa cagctttggt gcctttaccc tcggtcgagg acttcacgag gggtaatgat
    22981 ggctggagct tcgggctggg cctgggtgtt gagtacgaat ctgcctacga agggtcggat
    23041 gaattcggct tcgaagtcga tcctgctggc gcggttcaat ggcgcacagg tgatgatatt
    23101 ttctactggg cgggtgagac gctggggtgg cgtggtcttc gctccaagac gtggctattc
    23161 gacgccgccg tagccttcga cgagggtcgt gaagaaagtg attccgatga cggtcgattg
    23221 aatggactcg gcgatacaga tgagggtgct gaactcgtgc ttcaggcgcg tcgtgctttc
    23281 gatgccgatt ggcgttattg gctggatggt cgagtagtaa ccggtgaaaa cggcagtctt
    23341 ggtatctttg gggtggggcg ccgcttcggc gaccagaagg acggaaccgg ttctgaaatt
    23401 gccatcgttg tggtttatca cgatagcgat cgtgccaata cggattttgg catcaacgca
    23461 gcgcagtcag ctgcatcggg actgaatcag acccgtctga gtggaggatt ccgctcgatt
    23521 gggttccact acaactatcg tgattacatc aacaagaact ggcagatatt cggcgaggtg
    23581 ctctatgagc gttatggcag cgacattgag gacagcccaa tcgcacgcaa caactatgaa
    23641 gccgaagtgg gcgtcggatt catatacgtg ttctagaccg cctaacgtgc cgcatgacct
    23701 gcccgcaacg cgtgagcgga gcgaagcagg cgagggtcgg gggttcatgc gcctgaaact
    23761 tcgcgcgtat ctgtgggcga gatagccagc ctcgcagtca cccccgcagc agaaacctcg
    23821 ccgcctcctc cgcgatctcg tcgagcgact tgcgcccgcc cggacggtac caccgcgcgg
    23881 tccagttcaa cacgccaaac gtaaacaacc gcgccaactg cgcatccccg ggcatgaccc
    23941 cctgcgcctg cagctcgtcg gtcaccttct gccacagcgc gtcataccga tccttcaaag
    24001 caatcacccg ccgccgattc gccggcgtca acgaccgcca gtcgtagagc atcaccggaa
    24061 tgaagtcgtg cccgtcgccg cagatggtct ccaggtgcgt gcgcacgagc tggcggaatt
    24121 tgtccgcagg cggaagcttg cgcaaggcca cagcttcaag atggcgcaga ccctcggtca
    24181 ggccctgctc catcaccgcg accagcatct cctgcttcga cttgaagtgg tagaacgggc
    24241 tgccggagtg catgccggcg gcctcggaga tgtcgcggat tgacgtgccg tcgaagccct
    24301 tctcgcgaaa caggcgcgcg cacaccgcga gcagctcctc gcgccggttg ccgttctgcg
    24361 ccggcctttt cttcgccttc ttcacttcgt cttcagccgc ggcaggtcgg cgctcgcctc
    24421 gatctccatg cgcagcagct gcgccgcgac cgccttgccg agattgtcca tgcgcggcga
    24481 gcgccgggtt ggccttcagc gccggcaccg agccgtaggt catggtgacc gggcattgcg
    24541 tgccgttctc gacctcggaa tacagcaggt aggcggcggc gcgctcgacg tgcgcgccgg
    24601 ggccgcgttc gaggccatca ggttgtagtc ctcgagcggc gcgctctggt tgagcacttc
    24661 gtgcgtggcg gacatgcgcg atcgagtcta gacgcgcgcc ccttgaaaag aaattgcttg
    24721 gtagccgcgc acggtagaat cccgcgccgg aggaggtgcc gcatcgtcca gttcctgcag
    24781 ttgctgctcg cgcgcgtcgc gcagggttgc gtctacgcgc tcatcgcgct cggcacgatc
    24841 atccgcgaaa ccctcggcgc cgtcggcacg gcgaggaagc tcggctgggg cgtggacttc
    24901 gtgggctcgt cggccgccta caacgacctc atccacaagc tggacaagcc ggcggccaac
    24961 ggccaacggc ttctatgcca ccaacaccgt gaacaacccc tatctcgacg atgcctcgaa
    25021 gaacgtgcgc gagtgggcgc cgcgcgacat gttcggcgcg gacgcgatga gcttcaccca
    25081 gaccaagcac ctgggctcga accgggcgcg gctgtcgcag atcatcaacg gcaagtggac
    25141 gccggtcacc gaccacatca ccgagtaggg ggggtgttct gacgcgcacc gcgtcctggc
    25201 cgcgggcggc cgcggcgcgc atccgcacgc tgtgggtcct gaagatgctg tccacggcgg
    25261 gcggcatcgg cgtgttcttc tacgcgtact tctgggccat gcgccacccg ctcggcgccg
    25321 tgacggtgat gccggtgacc tgggtcgacg agctgatcgg ctttcagccg ctgtcctttc
    25381 cgctgtacgt gtccctgtgg atctacatct ctctgggcac cgcgctcgcc aaggatctgc
    25441 gcgagctggc gactttcggc gcggcgtcgc tcgccatgag cgtggtcggc ctcgccatct
    25501 tcatgctgct gccgacgcgc gcgcccgatt tcgcgatcga ctggtcgctg tacccgtcga
    25561 tgcagttcct gaaggaggtg gacgtcgctg gcaatgcctt tccttcgctg cacgcggcat
    25621 tctgcgtgtt caccgccgcg gtactgcatg cccagctgag cacgctgggt gccgggcgct
    25681 ggctgcgcgc cgccaacctg ctcttgtgcg ccggcatcct ctattccacg atggcgacgc
    25741 ggcagcacgt ggcgctggat gtcatcgcgg gcgccgttct gggcggggtg gcgtcggccg
    25801 cctatctcgg tgcgctgggc gagcgcaagc gcgcgcaagg gggctgaggt ggcgctgcgc
    25861 gtggtgcgct tgggcacgag gcgcgcggcc gacgagggtc cgcgcctcgg caccgtgcgc
    25921 cggccgcccc gcggcgtgcc cagggcgcgc ttcgcaaaag atgactggta tgacctgtgg
    25981 ctgcccgacc ttgcgcctag cgagccgctg gtgaagcagg cgctgcgcgc acaaagcaac
    26041 gcccagtggc gcgcgttcgc gaagaggtac cgcaaggaaa tggccgcgcc cgagaagacg
    26101 aggctgctcg aactgctcgc tgcgctgtcg aaggttgcca acttctccgt cggttgctac
    26161 tgcgaggacg aggctcgctg ccaccgctcg gtgctgcgca ggctcctcag ggagcgcggc
    26221 gcgaagctcg tgtcgtagcc ggccgcagga acagcggcac cgcagaaagg tcgagcgggg
    26281 ccacgccgcg ctcttccagg atgtgcaggg cgcgtgcctg caggcgccag cagctcgaac
    26341 gcagtgccgc ttgcgcggca tccacggaac cttcgagcga aagcgccgcg gtgcgcacgg
    26401 cgcagctggg gtgggtcgag agccggtcaa tcagcctgcg catctgcgcc gcgggggccg
    26461 ggcgcgcgcg gagcgccccg gcaatgccgc tggcgtggtg cggctcgtcg cgcagaacgt
    26521 cttcaaa
//
LOCUS       DBNHKE010000173         8013 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_665376, whole genome shotgun sequence.
ACCESSION   DBNHKE010000173 DBNHKE010000000
VERSION     DBNHKE010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_665376"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="AC16O9_11850"
     CDS             complement(<1..105)
                     /locus_tag="AC16O9_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517346.1"
                     /translation="MPRPPWRKRGMGGTFHGATVVTLDAKGRLAIPTRH"
     gene            complement(304..1380)
                     /locus_tag="AC16O9_11855"
     CDS             complement(304..1380)
                     /locus_tag="AC16O9_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIR6517347.1"
                     /translation="MPKSHVFQAAPLARAIEAIVLAGGSEPREAELVAENLVTANLTG
                     HDSHGIGMIPRYVESLKEGGLQPNRHPVMKLDGGALVALDGQAGYGQVIGLEATDIAI
                     ERAKQHGVCVMALANSHHLCRIGQWAEQAVAQGLVSLSFVNVISRSIVAPFGGSDARF
                     GTNPCTIGVPLAGQPPFILDMATSGVAQGKVRVAHNKGEKLPPGLLLDDKGNPTLEAR
                     FGVIEPFGALTTFGLHKGYGLAVVCELLGGALSGGGTWHTTDRSKKRVLNGMLSILID
                     PKKLGTADVFEREARAFIDWVKRSPPAPGMDRVRIAGDPERETRARREKEGIAVDANT
                     WQEIHAAGAKLGVEAAKLDRLAAG"
     gene            1423..2103
                     /locus_tag="AC16O9_11860"
     CDS             1423..2103
                     /locus_tag="AC16O9_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HIR6517348.1"
                     /translation="MIDKFIATAEQALADVPDGATIMIGGFGNAGMPAALIDTLIAQG
                     ATNLTVVNNNAGNADAGLAALIGMKRVKKILCSFPRQTDSWHFDKAFRAGEIELELVP
                     QGTLAERIRAAGAGIGAFYTPTAFGTRLAEGKETRRIGDIDYVLEYPIHADYALIKAD
                     RADRWGNLTYRMTARNFGPIMAAAARITVAQVRETVQLGELDPEVVVTPGIFVQRIVR
                     IPSLRQAA"
     gene            2100..2750
                     /locus_tag="AC16O9_11865"
     CDS             2100..2750
                     /locus_tag="AC16O9_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HIR6517349.1"
                     /translation="MKKLDRKQIAARLARDIPDGAYVNLGIGLPTLVADYLPRDREIV
                     LHSENGVLGIGPRPAPGAEDEDLINAGKEPVTLLKGGAFFHHADSFGMIRGRHLDYCV
                     LGAFQVSAKGDLANWHTGGADAIPAVGGAMDLAVGAKHVFVMMEHLTKDGKSKIVERC
                     TYPLTGAHCVDRIYSDLAVIDVKPDGLHVVEIVGELPFEELQRLTGVPLKRLATAA"
     gene            complement(2737..3696)
                     /locus_tag="AC16O9_11870"
     CDS             complement(2737..3696)
                     /locus_tag="AC16O9_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6517350.1"
                     /translation="MGFRRLLFAALALAASQALAQGYPNKPVRAIISFTPGSSTDIVG
                     RLVMAKVSEYWGQPVVAENRAGAGGSIGSNVVATAAPDGYMLLINSSAHAVNPAIYAK
                     LPYDTLKAFVDVVPLTSQPNVLVVGVNSPHKTVMDLVNYAKANPGKINFGHAGIGSGT
                     HLNTERFLAAADIKVTQVPFKGTPEVIAAILSNSVDCYWAPISAALANVKSGKLRPLA
                     VSTPKRNPQMPEVPTTGEAGVKGADSPLWFGVWAPAGTPADIVAKVNADVRKALADPG
                     VKEKLANLGNDAMDMSSGEFAKFVREEIETYGKVVRDAGIKPQ"
     gene            complement(3683..4603)
                     /locus_tag="AC16O9_11875"
     CDS             complement(3683..4603)
                     /locus_tag="AC16O9_11875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517351.1"
                     /translation="MPTPTRPSVGWIGAGRMGVEMAARLARGGADVLVWNRTRAKAEP
                     LAQYGAKIAANLSELAGRDIVFCMVATWKDVKQVMQALLAGSAQPRLVIECSSISLEG
                     SAELRALLAERGIDYLAAPVSGNAKVIKAGKLSFVCSGPRRSFDEALPLLKLIAPAAS
                     YVGEGELARIVKICHNVFLGVVIQSLAEITVLAEKAGVPRHAFLEFLNQSVMGSMFTR
                     YKTPAFVNLDFKVTFTPELLRKDMDLGLEAARRFEVPMPLAAAARDLIQSMMGRGMRE
                     EDFAALLVQQAEASGMELKPENVEVKDGLS"
     gene            complement(4587..5888)
                     /locus_tag="AC16O9_11880"
     CDS             complement(4587..5888)
                     /locus_tag="AC16O9_11880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6517352.1"
                     /translation="MKRKRAAPRIVRPDDVDPQHDWQRPLQAPGHTQVDFEERVNFRR
                     LHDYRLARTRAALAGSGLGALLSFDQHNIRYTTSTVIGEWARDKLTRYSLLTGNGDPY
                     IWDFGSAARHHKLHAPWLAQDHCRAGLLGMRGAVGADATLFRDAAREIKSILKAEGVA
                     KMPLGVDVVEPPMLFALQKEGIEVRDGQQAMLQAREVKNIDELTLLNMAAAMVDGVYQ
                     DIAEALKPGVQESQIVALATKRLYEMGSDCVEAINSISGERCSPHPHNFTDRLIRPGD
                     QAFFDVIQSFMGYRTCYYRTFNVGRATGPQRTAYRKAREWMDKVIELLRPGMSTDKVA
                     AALPKAQQAGFASEMDAFGLNFCHGLGLGLHERPLISRLNSFREPIELKAGMVFAVET
                     YCPATDGISAARIEEEVILTDKGAKIISLYPAEELPIANPY"
     gene            5924..7648
                     /gene="araD"
                     /locus_tag="AC16O9_11885"
     CDS             5924..7648
                     /gene="araD"
                     /locus_tag="AC16O9_11885"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HIR6517353.1"
                     /translation="MKKRPEELRSHRWFGVTDLRSFGHRSRTAQMGFDRSDYAGKPVI
                     AIINTWSDITHCHTHFRQRAEEVKRGVWQAGGFPLEMPAMALAETMQKPTTMLYRNFL
                     AMETEELLRSYPVDGCVLMGGCDKTTPALIMGATSMNLPAIYLPAGPMLNSHWRDQTL
                     GSGSDTWKYWDELRAGRITESDWQEIEDSIARSPGTCMTMGTAATMMSLAEALGFTLP
                     GYSSIPAPDSNHARMATLTGKRIVEMVWEDLKPREFLSKGSFDNAITTLMAMGGSTNA
                     IVHLVAMAGRAGLPFPLERYNEISARTPVIANVRPSGERYLMEDFYYAGGLRALLWQL
                     KDLLDLSAKTVNGRTLGENIDGAKVWNADVILPREKPLKASGGIVMLRGSLAPDGAVI
                     KTSAADPKLMRHTGRAVVFEDYNDMAARIDRDDLEVDASSVLVLKNAGPLGGPGFPEW
                     GMLPMPKKLVKQGVKDMVRLSDGRMSGTSYGTCILHVAPEAYVGGPLAFARTGDLIEL
                     DVEKKLLNLKISEAELAKRKAAWKRPAPRYERSYGAIFSRHVKQANEGCDFDFLEGTA
                     PTAEPEIH"
BASE COUNT         1305 a         2770 c         2647 g         1291 t
ORIGIN      
        1 atggcgcgtg gggatggcga gccggccctt tgcgtccagc gtgaccaccg ttgcgccgtg
       61 gaacgttcca cccatgcccc gttttctcca aggcggacgg ggcatgaatc acgcctcccc
      121 gtgttccgcc acttttttct acctttttcc actctatgcg acaaaagggg cgacggtcaa
      181 ggcaattctt tttgcctgac aatgacttag ctgcgtttcc tgggggcgtt tcttgaaata
      241 aaactactat atatatcagt agcttataaa agctactgaa agtgctgtct tagaactacg
      301 gccttagccg gcggccagac ggtcgagctt cgcggcctcc acgcccagct tggccccggc
      361 cgcatggatc tcctgccagg tgttggcgtc gacggcgatg ccctcctttt cgcggcgcgc
      421 gcgcgtctcg cgctccggat cgcccgcgat gcgcacgcga tccatgcccg gcgcgggcgg
      481 cgagcgcttc acccaatcga tgaatgcgcg cgcctcgcgc tcgaacacgt ccgcggtgcc
      541 gagcttcttc ggatcgatga ggatcgagag catgccgttc agcacgcgct tcttcgagcg
      601 gtcggtcgtg tgccaggtgc cgccgcccga gagcgcgccg ccgagcagtt cgcacacgac
      661 ggcgagccca tagcccttgt gcagcccgaa agtcgtcagc gcgccgaagg gctcgatcac
      721 gccgaagcgc gcctccagcg tcgggttgcc cttgtcgtcg agcagcagcc ccggcggcag
      781 cttctcgccc ttgttgtgcg cgacgcgcac cttgccctgc gccacgccgc tcgtcgccat
      841 gtcgaggatg aacggcggct gcccggcgag gggcacgccg atggtgcagg gattggtgcc
      901 gaagcgcgcg tcgctgccgc cgaagggcgc gacgatggag cgcgagatca cgttgacgaa
      961 gctgagcgag accagcccct gcgccaccgc ctgctcggcc cactggccga tgcggcacag
     1021 atgatgcgag ttggcgagcg ccatcacgca cacgccatgc tgcttcgcgc gctcgatggc
     1081 gatgtcggtg gcctccagtc cgatcacctg cccgtagccc gcctggccgt cgagcgcaac
     1141 gagcgcgccg ccgtcgagct tcatcaccgg gtggcgattg ggttgcagcc cgccttcctt
     1201 cagcgattcg acatagcgcg ggatcatgcc gatgccgtgc gaatcgtggc cggtgaggtt
     1261 ggcggtgacg agattctcgg cgacgagctc ggcttcgcgc ggctcgctgc cgccggcgag
     1321 gacgatcgcc tcgatggcgc gcgcgagcgg cgcggcctgg aagacgtggg atttgggcat
     1381 gggggcggag tttataatgg cccggaccgc agcgacgccg ccatgatcga caaattcatc
     1441 gctactgccg aacaggcact cgccgacgtc cccgacggtg ccaccatcat gatcggcggt
     1501 ttcggcaacg cgggcatgcc tgccgcgttg atcgacacgc tgatcgcgca aggggcgaca
     1561 aacctcacgg tggtcaataa caacgccggc aacgccgacg ccgggctcgc ggcgctgatc
     1621 ggcatgaagc gcgtcaagaa gatcctgtgc tccttcccgc ggcaaaccga ctcctggcat
     1681 ttcgacaagg cgtttcgcgc cggcgagatc gagctggagc tcgtgccgca gggcacgctc
     1741 gccgagcgca tccgcgccgc cggcgccggg atcggcgcgt tctacacgcc gacggcgttc
     1801 ggcacgcgcc tcgccgaggg caaggaaacg cgccgcatcg gcgacatcga ctacgtgctc
     1861 gagtacccga tccacgccga ctacgcgctg atcaaggccg atcgcgccga ccgctggggc
     1921 aacctcacct accgcatgac cgcgcgcaat ttcgggccga tcatggccgc cgccgcgcgc
     1981 atcaccgtgg cgcaggtgcg cgagaccgtg cagctcggcg agctcgatcc cgaggtggtg
     2041 gtgacccccg gcatcttcgt gcagcgcatc gtgcgcatcc cttcgctgag gcaagccgca
     2101 tgaaaaagct cgaccgcaag cagatcgccg cgcgcctcgc gcgcgacatc cccgacggcg
     2161 cctacgtgaa cctcggcatc ggcctgccga cgctggtggc cgactatttg ccgagggacc
     2221 gcgaaatcgt cctgcacagc gagaatggcg tgctcggcat cggcccgcgc ccggcgccgg
     2281 gcgcggaaga cgaggacctg atcaacgccg gcaaggagcc ggtgacgctg ctgaagggcg
     2341 gcgcgttctt ccaccacgcc gactcgttcg gcatgatccg cggccggcac ctcgattact
     2401 gcgtgctcgg cgccttccag gtctcggcaa agggcgacct cgccaactgg cacacgggcg
     2461 gcgcggacgc gattccggcg gtcggcggcg ccatggacct cgcggtgggc gctaagcacg
     2521 tcttcgtcat gatggagcac ctgacgaagg acgggaaatc caagatcgtc gagcgctgca
     2581 cctatcccct cacgggcgcg cactgcgtcg accgcatcta cagcgatctc gcggtgatcg
     2641 acgtgaagcc cgacgggctg cacgtggtcg agatcgtggg ggagctcccg ttcgaggagc
     2701 tgcagcgcct gacgggcgtc cccctgaaac gcctcgctac tgcggcttga tgccggcgtc
     2761 gcgcaccacc ttgccgtagg tctcgatctc ctcgcgcacg aacttcgcga actcgccgct
     2821 cgacatgtcc atcgcgtcgt tgcccaggtt ggcgagcttc tccttcacgc ccggatcggc
     2881 cagcgccttg cgcacatcgg cgttcacctt cgcgacgatg tccgccggcg tgcccgcggg
     2941 cgcccacacg ccaaaccaca gcggcgagtc cgcgcccttc acgcccgcct cgcccgtcgt
     3001 cggcacctcc ggcatctgcg ggttgcgctt cggcgtgctg accgcaaggg gacggagctt
     3061 cccgctcttc acgttcgcca gcgcggccga gatcggcgcc cagtagcagt ccacggaatt
     3121 gctgaggatc gccgcgatca cctccggcgt gcccttgaag ggcacctgcg tcaccttgat
     3181 gtcggcggcg gcaagaaagc gctcggtgtt cagatgcgtg ccgctgccga tgccggcgtg
     3241 gccgaagttg atcttgcccg ggttcgcctt cgcataattg accaggtcca tgaccgtttt
     3301 gtgcggcgag ttcacgccga ccaccagcac gttgggctgc gaggtgagcg gcaccacgtc
     3361 gacgaaagcc ttcagcgtgt cgtagggcag cttggcgtag atcgcgggat tcacggcatg
     3421 cgccgacgag ttgatcaaca gcatgtagcc gtcgggggcg gcggtggcga cgacgttcga
     3481 gccgatcgag ccgccggcgc cggcgcgatt ctcggcgacc accggctggc cccagtactc
     3541 cgacaccttg gccatcacca ggcgcccgac gatgtcggtc gagctgccgg gcgtgaacga
     3601 gatgatcgcg cgcaccggct tgttgggata gccctgcgcc agcgcctgcg aagcggcaag
     3661 cgcgagcgcc gcgaacagca atctacgaaa gcccatcttt gacctccacg ttttcaggtt
     3721 ttagttccat gccggaggcc tcggcctgct gcacaagcag cgcggcgaaa tcctcctccc
     3781 tcatgcccct tcccatcatc gactggatca ggtcgcgcgc ggccgcggcg agcggcatcg
     3841 gcacttcgaa gcggcgcgcc gcctccagcc ccaggtccat gtcctttctc agcagctccg
     3901 gcgtgaacgt gaccttgaaa tcgaggttga cgaacgccgg cgtcttgtag cgcgtgaaca
     3961 tcgagcccat cacgctctgg ttgagaaact caaggaacgc gtgccgcggc acccccgcct
     4021 tctcggccag caccgtgatc tcggcgagcg actggatcac cacgccgaga aatacgttgt
     4081 ggcagatctt gacgatgcgc gcgagctcgc cctccccgac gtaactcgcc gccggtgcga
     4141 tcagcttcag aagcggcagc gcctcgtcga acgacctgcg cggccccgag cagacgaacg
     4201 agagcttgcc cgccttgatc accttggcgt tgcccgacac gggcgcggcc agatagtcga
     4261 tgccgcgctc cgcgagcagc gcgcgcagct cggcggagcc ttcgagcgat atcgacgagc
     4321 actcgatcac caggcgtggc tgcgcgcttc ccgcgagcag cgcctgcatc acctgcttga
     4381 cgtccttcca agtggcgacc atgcagaaca cgatgtcgcg gcccgcaagc tcggaaaggt
     4441 tggcggcgat cttcgccccg tattgcgcga gcggctcggc cttggcgcgc gtgcggttcc
     4501 acaccagcac gtcggcgccg cctcttgcga ggcgcgcggc catctcgacg cccatgcgtc
     4561 ccgcgccgat ccagccgacc gagggcctag taggggttgg caatgggaag ctcctcggcc
     4621 gggtacagcg agatgatctt tgcgcccttg tccgtcagga tcacttcctc ttcgatgcgc
     4681 gccgccgaga tgccgtcggt ggcggggcag taggtctcga ccgcgaacac catgccggcc
     4741 ttcagctcga tcggctcgcg gaacgagttg agccgcgaga tcagcggccg ctcgtgcagc
     4801 ccgagcccca ggccgtggca gaagttgagg ccgaacgcat ccatctcgct cgcaaagccg
     4861 gcctgctgcg ccttcggcag cgcggcggcg accttgtcgg tgctcatgcc gggcctcagg
     4921 agctcgatca ccttgtccat ccactcgcgc gccttgcggt aggcggtgcg ctgcgggccg
     4981 gtggcgcgcc cgacgttgaa agtgcggtag tagcaggtgc ggtagcccat gaacgactgg
     5041 atcacgtcga agaacgcctg gtcgcccggg cggatcagcc ggtcggtgaa gttgtgcggg
     5101 tgcggcgagc agcgctcgcc cgagatcgag ttgatcgcct cgacgcagtc cgagcccatc
     5161 tcgtacaggc gcttggtggc gagcgccacg atctgcgatt cctgcacgcc gggcttcagg
     5221 gcctcggcga tgtcctggta gacgccgtcc accatggctg ccgccatgtt gagcagcgtc
     5281 agctcgtcga tgttcttcac ctcgcgcgcc tgcagcatcg cctgctggcc gtcgcgcacc
     5341 tcgatgccct ccttctgcag cgcaaagagc atcggcggct cgaccacgtc gacgccgagc
     5401 ggcatcttcg ccacgccttc ggccttcagg atcgacttga tctccctcgc cgcgtcgcga
     5461 aacagcgtcg cgtccgcgcc gaccgcgccg cgcatgccga gcagccccgc gcggcagtgg
     5521 tcctgcgcga gccacggcgc gtgcagcttg tggtggcgcg cggccgagcc gaagtcccag
     5581 atatacgggt cgccgttgcc ggtgagcagc gagtagcgcg tcagcttgtc gcgcgcccat
     5641 tcgccgatca cggtgctcgt ggtgtagcgg atattgtgct ggtcgaagga gagcagcgcc
     5701 ccgagccccg acccggcgag tgcggcacgc gtgcgcgcca gccggtagtc gtgcagccgg
     5761 cggaaattga cgcgctcctc gaaatcgacc tgcgtgtggc cgggcgcctg cagcgggcgc
     5821 tgccagtcgt gctgcgggtc gacgtcgtcc ggccggacga ttctcggcgc cgcgcgcttt
     5881 cgcttcatgg tctcctccgg cgcggagtat atacaatcgc cgcatgaaaa agcgccccga
     5941 ggaactgcgt tcccaccgct ggttcggcgt cacggacctg cgttcgttcg gccaccgctc
     6001 gcgcaccgcg cagatgggct tcgaccgctc ggattacgcc ggcaaacccg tcatcgccat
     6061 catcaacacg tggtcggaca tcacccactg ccacacccac tttcgccagc gcgccgagga
     6121 ggtgaagcgc ggggtgtggc aggcgggcgg gttcccgctg gagatgccgg cgatggcgct
     6181 cgccgagacc atgcagaagc cgaccaccat gctctaccgc aacttcctcg ccatggagac
     6241 cgaggagctg ctgcgctcct accctgtgga cggctgcgtg ctgatgggcg gctgcgacaa
     6301 gaccacgccc gcgctcatca tgggcgcgac cagcatgaac ctcccggcga tctacctgcc
     6361 cgccgggccg atgctgaaca gccactggcg cgaccagacg ctcggctcgg gctcggacac
     6421 ctggaagtac tgggacgagc tgcgcgcggg ccgcatcacg gaaagcgact ggcaggagat
     6481 cgaggactcg atcgcgcgct cgcccggcac ctgcatgacg atgggcacgg cggcgaccat
     6541 gatgtcgctg gccgaggcgc tcggcttcac cctgcccggc tactcctcga tcccggcgcc
     6601 cgactcgaac cacgcgagga tggcgacgct cacgggaaag cgcatcgtcg agatggtgtg
     6661 ggaggacctg aagccgcgcg agttcctgtc gaagggctcg ttcgacaacg ccatcaccac
     6721 gctgatggcg atgggcggct cgaccaacgc catcgtgcac ctcgtcgcca tggcgggccg
     6781 cgcggggctg ccgtttccgc tggagcgcta caacgagatc tcggcgagga cgccggtgat
     6841 cgccaacgtg cggccctcag gcgagcgcta cctgatggag gatttctact acgccggggg
     6901 cttgcgcgcg ctgctgtggc agctgaagga tctgctggat ctctcggcga agacggtgaa
     6961 cgggcgcacg ctcggcgaga acatcgacgg cgcgaaagtg tggaacgcgg acgtgatcct
     7021 gccgcgcgag aaaccgctga aggcctccgg cggcatcgtc atgctgcgcg gctcgctcgc
     7081 gcccgacggc gcggtgatca agacgagcgc cgccgatccg aagctgatgc ggcacaccgg
     7141 ccgcgccgtg gtgttcgagg actacaacga catggcggcg cgcatcgacc gcgacgacct
     7201 cgaggtcgac gcgagtagcg tgctcgtgct gaagaacgcc gggccgctcg gcggccccgg
     7261 ctttcccgag tggggcatgc tgccgatgcc caagaagctc gtgaagcagg gcgtgaagga
     7321 catggtgcgg ctgtcggacg gccgcatgag cggcacgagc tacggcacct gcatcctgca
     7381 cgtcgcgccc gaggcctacg tcggcgggcc gctggcgttc gcgcggaccg gcgacctgat
     7441 cgagctcgac gtggaaaaga agctcctgaa cctgaagata tcggaggccg agctggcgaa
     7501 gcgcaaggcc gcctggaagc ggcccgcgcc ccgctacgag cgcagctacg gcgcgatctt
     7561 ctcgcgccac gtgaagcagg cgaacgaggg ctgcgacttc gacttcctcg agggcaccgc
     7621 gcccacggcg gagcccgaga tccactgatg cacgggtcgg atagcaggat ctatccgcac
     7681 gagcccatac gacatgcccc gaaatgcccc cattgcgtga cctagcccgc gacgagcgct
     7741 gacgcgaagt aaggcagcgc cgcgtcgttc acggcggcgt cgcgcctggc tcggcggttc
     7801 ctactgttcg ttccggtgct ttccccggaa tgcccccaca gcaccacccg cccgcggccg
     7861 atgagaatga agcgcgcgag cgctcacagc ccctcggcgc tcatgcgccg ggccgcgccg
     7921 tgtcgtcgaa gcgccggccg atccggcgaa tgaatcccgc caccatccac agcatgaggc
     7981 cgagcaggac gaatgcggcg gcgatgaccc agg
//
LOCUS       DBNHKE010000174        25007 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1406002, whole genome shotgun sequence.
ACCESSION   DBNHKE010000174 DBNHKE010000000
VERSION     DBNHKE010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 25007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1406002"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="AC16O9_11890"
     CDS             complement(<1..1586)
                     /locus_tag="AC16O9_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6517354.1"
                     /translation="MRFAIFALLAALAPAAAGQAGDAVVITAPRFPEDVRRLPASVTV
                     LDAAAIARSAARTLPELLAEQPGINMRDFFGNNAAGTAIDLRGFGITGAQNTLVLVDG
                     RRISDFDLSGVQWAAIPLATIERIEILRGTGAVLYGDGATAGVVNIVTRSPLKQGPRA
                     EALGRIASYGTAEGQLYGARAGESFGINASIYGYDADGYRRNNRNRQQNLATNMRWGL
                     GESYADLRFGMDRQEMRLPGARSVRPSIGLNEYATDPRGAQTPLDWSERDGNRAGATL
                     AHRVGEAELTLGLDWRDKEQRSYFDQQGPVARDERLQWGSIGPRARFPLQLASVRHRL
                     TLGADANAWRYDSRRADRPENLGQPVNRVRVNQDAVALYVQDAMQLSAESLFTLGWRE
                     ERVKYRASDAFDPTSPGYPGSGSQAPAVSETQRQHAWEAGWRQALSPVWTWFARAGRS
                     YRFVNAEEIYESDAAFDAEFQILRPQHARTVEAGAEWREQSASVRATLFHADITDEIH
                     LDPFSAGIGNTNLPPTRRRG"
     regulatory      complement(1627..1865)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1884..2762
                     /locus_tag="AC16O9_11895"
     CDS             1884..2762
                     /locus_tag="AC16O9_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR6517355.1"
                     /translation="MTRTMLGALMLAACAAQAEVRVTDDYGKPVVLAAPARRVVSLAP
                     HLTELMYAAGAGERMVGAVQYSDYPDDAKHLPRIGSEASIDLEAVVALGPDLVVAWPN
                     AGSARAVERIAALGIPVFRSEPRELEDIARTLEILGRLAGTETAARPAAAAFRARTAT
                     IAHRHASRPPVRVFYQVWDRPLITVNGDHVISKVMRLCGGENVMADLPALAPEIDREL
                     VLRADPEAIVASGADGARPPWLDAWRAFGGLAAVRGDNLYAIRPELLQRHTPRLLDGA
                     DELCRILEAVRRRRAL"
     gene            2839..3030
                     /locus_tag="AC16O9_11900"
     CDS             2839..3030
                     /locus_tag="AC16O9_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517356.1"
                     /translation="MEAELNSLEAKVAQFVGVCERLRAENIALRQELATARNDAKRLN
                     EKIEGARSKVEQLLTRLPE"
     gene            3027..3359
                     /locus_tag="AC16O9_11905"
     CDS             3027..3359
                     /locus_tag="AC16O9_11905"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR6517357.1"
                     /translation="MSANEKRAADGAKTIEVTLLGRGYRVACSDEEREALLQAVAYLE
                     GKMNEIKQAGKVAGTERIAVMAALNIAHELLSMRLGEGFDMGEAKRRISLIEARLEAA
                     LAKQEKLF"
     gene            3376..3562
                     /gene="ssrS"
                     /locus_tag="AC16O9_11910"
     ncRNA           3376..3562
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16O9_11910"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3594..4052
                     /locus_tag="AC16O9_11915"
     CDS             3594..4052
                     /locus_tag="AC16O9_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR6517358.1"
                     /translation="MPFWLMKSEPDECSIDDALAAPGRITPWTGVRNYQARNFMRDAM
                     RVGDGVFFYHSSCPEPGIAGLAEVASAPYPDPTQFDRRTDYYDAKSKRTSPRWAMVDV
                     RALKKIPLVPLAELRRHKALAKMWLLRPGSRLSITPVSQVEWRYITGKLL"
     gene            4022..5113
                     /gene="hemE"
                     /locus_tag="AC16O9_11920"
     CDS             4022..5113
                     /gene="hemE"
                     /locus_tag="AC16O9_11920"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR6517359.1"
                     /translation="MALHHRKAPLTNDTLLRALACEPTAYTPVWLMRQAGRYLPEYRA
                     TRERAGSFLALAKSPALATEVTLQPLERFALDAAILFSDILTVPDAMGLGLHFAEGEG
                     PRFARPLRDEAAIRALAAPDPSDRLRYVIDAVREIRKALAGRVPLIGFAGSPFTLACY
                     MVEGAGSDDWRALKTMLYARPDLLHRILEVNTRAVTDYLNAQIEAGAQVVMLFDTWGG
                     QLSHAGYEAFSLAYARRIFAALKRESGGRKVPAILFTKGGGAWLEAMASSGCDALGLD
                     WTVDPAAARARVGPRVALQGNLDPAALFAPPEAVRAEARRVLDAFGAAPGHVFNLGHG
                     ISQHTPIESVAALVDEVRTYSSKLRPATK"
     gene            5329..7362
                     /locus_tag="AC16O9_11925"
     CDS             5329..7362
                     /locus_tag="AC16O9_11925"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR6517360.1"
                     /translation="MPFRSEAYTLARSPMTVLRVALDVPLATLFDYAPADEGVARVGD
                     RVVVPFGTRARVGVVVEEGTSSTIAAAKLKRVARVLDDAPRLPAPWLELMRFLAAYYQ
                     RPLGETVIGALPPRLRSLKPLPKKARAPAAADPASPHFVSPHQPTAAQAQAIERIGGA
                     LGGFKAFLLHGVTGSGKTEVYLRLISQVLERGGQALVMVPEISLTPQLEARFRRAFPG
                     ARVALLHSALEDVPRTAAWLAAARGEAGLVLGTRLAVLTPLPNLALVVVDEEHDTSFK
                     QQEGLRYSGRDAAVVRAKHAACPVVLGTATPSLETWQNAIEGRYERLALPERAVPGAR
                     LPAVRTIDLRREGAEHGFAPSLFAAIGERLPRGEQSLVFINRRGYAPVLACHACDWTA
                     GCKRCTARMVLHRVGHCLRCHHCGDEAPVPRACPTCGNLDLKAMGRGTQRVEDTLGAR
                     FPGARIVRIDRDSARRRSELSRTLEGIARGEADILVGTQLLAKGHDFPLLTLVGVLDA
                     DNALVSTDYRAAERLYSTLAQVAGRAGRARHAGEVLVQTRYPQHPLYAALARHDYATF
                     AAAQLAERESAGFPPFVHEAALRAEAPKLALALQFLEDAARLVPVPQEVRVYDPVPHV
                     ITRRADLERAQLLVQSASRPALQGFLAAWTERLFAAAPRGVRWHLDVDPIEFS"
     gene            7399..9027
                     /gene="argS"
                     /locus_tag="AC16O9_11930"
     CDS             7399..9027
                     /gene="argS"
                     /locus_tag="AC16O9_11930"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR6517361.1"
                     /translation="MDIKAHLATLLRAALVRAAPEAADAQVLIERPRDVAHGDFACNV
                     ALQLAKRLKRKPRELAERLTAAVAADPYVAKLEVAGAGFINIRLAPRAGQEAVARVLQ
                     LGAAYGHARAAQAPKTMVEFVSANPTGPLHVGHGRQAALGDALAALLASQGHDVTREF
                     YYNDAGAQIHNLALSVQARARGTKPGDPGWPADGYRGEYIEEIARSFTAAGGDVGDVE
                     AVRTFAVAALRREQDADLQAFGVKFDVYYLESSLYTDGRVDAVVKALVASGKTFEQEG
                     ALWLRTTDYGDDKDRVVRKSDGSYTYFVPDVAYHVAKWERGFAKVINVQGTDHHSTVT
                     RVRAGLQALSLGIPSGYPDYVLHNLVRVIRGGEEVKISKRAGSYVTLRDLIDWVGRDA
                     VRFFLVSRRADSDFVFDVDLARSQSEENPVYYVQYAHARVCSVFSQWGGDAAPLPGAP
                     LGALTGGREQALMRRLMEFPEMLADAAHELVPHAIAFYLRELAGEFHSYYNAERILVE
                     DDATRTARLALCAAVRQVLANGLALLGVRAPERM"
     gene            9027..9683
                     /locus_tag="AC16O9_11935"
     CDS             9027..9683
                     /locus_tag="AC16O9_11935"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6517362.1"
                     /translation="MAAIPHATARKGSGGGFLVGMFVGLLLGLGIALGIALYLNKTPI
                     PFMSRAKPLAEDGKGEAGKPSALAGLPQGGDKPAAQVPVKPPEKPRFDFYKILAGGEE
                     PLSDRELRERLKAAKTPEPAQTAAVAPAEGKDIYFIQAGAFQNPADADNQKARLAMLG
                     FESNVEPTNLPDKGMFYRVRLGPYAKVEELNRVRQSLAQNGIDANLVKLKDPATRKVN
                     "
     gene            9684..10316
                     /locus_tag="AC16O9_11940"
     CDS             9684..10316
                     /locus_tag="AC16O9_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6517363.1"
                     /translation="MDFRRRTLVAALGLAPLAGLAQARRPTPGVDFSELRPAQPVEAQ
                     GKIEVIEFFWYGCPHCYSLQPLIEKWVPRLAADVNFRRVPAVLNEGWAREAAVFYAFE
                     ALGVLEKLHRPFFDAIHVDRISTRNPATLAEWLRKQEVDVQKYEDAFKSFGVQSKVRR
                     AAQLSVAYRIDGTPALAVHGRYTVSAEQGRTQAGMLSTADYLIGVARKGA"
     gene            10337..11086
                     /locus_tag="AC16O9_11945"
     CDS             10337..11086
                     /locus_tag="AC16O9_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6517364.1"
                     /translation="MTGASDGLGAALARHYASPDTVIGLIARRGEPMQALAAGLPGRF
                     EIYALDVADHGALERAAKEFCARHGAPDLVVANAGLSVGTRGEDSADLVKLRRLLEVN
                     VTGLAATLAAFAPAMRAARAGTLCGIASVAAFRGLAGAGAYSASKSAAIAWLEALRVE
                     MAGSGVCVVTICPGYIDTPMTRVNRYRMPFLLSADEAARRIARAIRARRRLAVIPWQM
                     ALVSVVLRALPGWLYDRLFARGPRKPRDLPL"
     gene            11083..11814
                     /locus_tag="AC16O9_11950"
     CDS             11083..11814
                     /locus_tag="AC16O9_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HIR6517365.1"
                     /translation="MTRIVSLVPSLTELVCALGLGAALVGRTGFCVHPKDVVRRIPKV
                     GGTKDVNLDKVRALAPTHVVLNVDENRKETADELATFVPNLVVTHPLGPLDNLALYRQ
                     FGRTFAREREAEALCARFQAAYDEARGLRLPERRALYLIWKDPWMTVSRDTYISRTLA
                     IFGLNTVPEAAAQRYPKLEDLASVDADLVLLSSEPYRFRDRHVAQVAGITGKPALLVD
                     GEMTSWYGPRAIAGLDYMAKFAAHA"
     gene            11882..12934
                     /locus_tag="AC16O9_11955"
     CDS             11882..12934
                     /locus_tag="AC16O9_11955"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6517366.1"
                     /translation="MDGTRILKRSALAGVASLLCMYGTASWAVNDEPLKDKWAPSEFG
                     KDDKAGAANRRTAASVLKAAKLVKKGKVATLGKVYQQDAPGFGSRGWRLVIPGLPTGG
                     PFGKQELLYNDEYVATEIGQIGTQFDGPGHIGVHTSKGMFFYNGRYLHDEGVSTYGMG
                     PLGVEHVGQLGYACRGVLLDAVALRGGKQLPIPAKAGDPGIITDKDVEAMVKRQGIAP
                     IGEGDCVFLHTGHGDIWHPSKWDSFSAEEKQKRIAHFNSGEPGFGISACEYLAKRKVA
                     LVGADVWAVEAVPGEGEQPFECHIKMQTKRGIWFLENMDLSQLVADKAYEFLFAWSPL
                     KIKGGTGSPGNPVAIY"
     gene            complement(12948..14039)
                     /locus_tag="AC16O9_11960"
     CDS             complement(12948..14039)
                     /locus_tag="AC16O9_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR6517367.1"
                     /translation="MLSREDNALLTRVGPGTVMGALFRRHWLPFMLADELVLESAPQR
                     VRLLGESLVAFRDNRGRPGLLDENCPHRRASLCLGRNEAGGLRCAYHGWKFDVSGRCL
                     EMPSEPAGSAFRDKVRAKAYPVTERAGLLWAYLGPAEKRAELPELEWLGLPAAQRNAS
                     RRVYECNYAQALEGEIDESRFGLAGADDPAPRYTVHEAPYGLACGVRRSVQGEQHLWR
                     ISLFLMPCFTLVPPADDPDSRLFRAWVPEDDERTSVLSVNWRTDGPASARQRELWQRA
                     DAAHLGLAVSAGAVADRTREHLGTSDKAVVALRQRLLGAARRLAEGLEPQTPYDPDCY
                     RVRAHAGRVPRDERDFPEQEEIRRAMLAP"
     gene            complement(14109..15896)
                     /locus_tag="AC16O9_11965"
     CDS             complement(14109..15896)
                     /locus_tag="AC16O9_11965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR6517368.1"
                     /translation="MGESADGINREETAKVLHEVAERSSRILKEFGQKQLQSTISSAV
                     KDELGIARAYMDLYSRLLMDPSALAAASVNFWIDSMRLWQSSWLKMMGQAPAPVAQPA
                     KGDVRFKDQEWTDNFLFDFIKQSYLIAARHTQHAVAGVQGLPEESQKKIAFFTRQYVD
                     ALAPSNFAATNPQVLRETVASGGQNLVKGLNNLLADIEKGEGQLRISMTDEDAFQLGR
                     NVATTPGKVVFQNALLQLIQYQPSTPDVFRRPLLVIPPWINKYYILDLREKNSFIKWA
                     VDQGHTLFVVSWVNPDAKLAQKGFDDYLTEGALAAMDAVMRATGEKHLNVIGYCLGGT
                     LLGCALAYLAGKGDARVGCATFFVSLLDFSQPGELGVFIDEAQVDNLERRMNERGYLE
                     GSEMATTFNMLRSNDLVWSFVINNYLMGKDPFPFDLLYWNSDSTRMPARMHSFYLRNM
                     YLKNKLGVPGAVTLAGEPIDLSKVKVPAYFISTVEDHIAPWKTTYEGAKYLGSEVRFV
                     LGGSGHIAGIVNPPAAKKYHYWTNDSFADTPDAWFEGATQHPGSWWEDWQRWIAQQNR
                     GDKVPARIPSKAIEDAPGSYVMQRLSKKK"
     gene            complement(15872..16927)
                     /locus_tag="AC16O9_11970"
     CDS             complement(15872..16927)
                     /locus_tag="AC16O9_11970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6517369.1"
                     /translation="MSALEFPHPEAPAPGAVREIAPGVRWLRMRLPFALDHINLWMLE
                     DGDGWTLVDTGLADAPTRAQWEQVFAATGARAVRRVLVTHFHPDHAGNAGWLCERFGA
                     PLWITQGEYLTAHAVRAGAAGFVAEATLGLFRAHGLDEARLGQLGMRGNQYRRLVGEL
                     PTQYRRILEGESIGVGGRAWRVITGYGHAPEHASLYCEALNVVISGDMLLPKISTHVG
                     VWPTDPEGDPLGYFLRSIRRYRELPQDVLVLPSHGLPFRGAQARVAQLEEHHETRLAE
                     LLTACGEAPRSAADVLALIFRRPLDTQQMFFAMGEAIAHLHYLERAGRLRRMRGEDGV
                     SRFALIKEKAWEKALTA"
     gene            16964..17371
                     /locus_tag="AC16O9_11975"
     CDS             16964..17371
                     /locus_tag="AC16O9_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6517370.1"
                     /translation="MRQIEKERADAEYSIGELAAEFDVTPRAIRFYEAEGLLAPRREG
                     QRRIYTPRERTRLKLTLRGKRLGLTLSEIRDLIDMYEPGQDQRPQLERFLAVLETHKA
                     ALERQREDIAVQLAEIAAFEKRVRKQLAARRRG"
     gene            17404..17871
                     /locus_tag="AC16O9_11980"
     CDS             17404..17871
                     /locus_tag="AC16O9_11980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6517371.1"
                     /translation="MHAPQPRFQPRDPGYAARVQESFGRQKAMQHIGARLRAVGPGWT
                     EVELPHRDELTQQKGFLHGGIIGMIADNACGYAAFSLMPAGASPLTVEYKINILAPGR
                     GDRYVAEGVVLRSGRTLSVTRAEVYAETAAGRVHVASMQQTVLMLADTPDTPE"
     gene            17868..19025
                     /locus_tag="AC16O9_11985"
     CDS             17868..19025
                     /locus_tag="AC16O9_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIR6517372.1"
                     /translation="MNFLRFEHGETLDMLRDTVRAFAQKEIAPRADEIDRANEFPADL
                     WKKFGALGLLGITVEEAYGGTMMGYLAHIIAMEEISRASAAVGLSYGAHSNLCVNQIR
                     RNGSDAQKKKYLPKLVSGEHVGALAMSEPGAGSDVVGMRLRAQRVKSGRGDAYVLNGN
                     KMWITNGPDADVLVIYGKTSDQGITAFLVEKGMKGFSTAQKLDKLGMRGSNTCELVFR
                     DCEVPAGNVLGEEGKGVKVLMSGLDYERAVLSGGPLGVMAACLDVVLPYVHERKQFGQ
                     PIGEFQLMQGKLADMYTTFSACRAYVYAVGQALDRGETTRKDAAGAILYAAEKATWMA
                     GEAIQALGGMGYINETPTGRLWRDAKLYEIGAGTSEIRRWLIGRELFGEST"
     gene            19022..19615
                     /gene="can"
                     /locus_tag="AC16O9_11990"
     CDS             19022..19615
                     /gene="can"
                     /locus_tag="AC16O9_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HIR6517373.1"
                     /translation="MKLQQLLENNRAWAREALAADPEYFARLARQQSPQFLWIGCSDS
                     RVPANQITGLAPGEVFVHRNIANVVVHTDLNCLSVIQFAVDFLKVRHIILCGHYGCSG
                     VAAAMSQQRTGLSENWLRHVQDVAAKHGGELRAIQSDDARYRRLCELNVREQVHHISQ
                     CTSVQDAWARQQPLAVHGWIYDLQDGLLRDLDCTRTE"
     gene            19620..20804
                     /locus_tag="AC16O9_11995"
     CDS             19620..20804
                     /locus_tag="AC16O9_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6517374.1"
                     /translation="MAHDPIVIVSAARTPMGGFQGELKSFAAPELGAAAIRAAVERAK
                     VKPEDVQEVLMGCVLPAGQGQAPARQASLGAGLPLAAGCTTVNKMCGSGMKAAMLASD
                     LLAAGSMDIVVAGGMESMTNAPYLLPKARAGLRMGHGQVLDHMFFDGLEDAYDKGRLM
                     GTFAEDCAQKYAFSREAQDRFAIASLERAQKANQEGWFAWETTPLAVKAGKEEKFIET
                     DEQPFKANLAKIPTLKPAFRKDGTVTAANSSSISDGAAALVLMRRSTADKRGLAPIAT
                     VAGHATHAQEPGWFTTAPVGAMRKLFERTGWSAGKVDLFEINEAFAVVAMAAMKEHGL
                     PHEKVNVHGGACALGHPIGASGARILVTLIGALRKYGGRRGVASLCIGGGEATAMALE
                     LA"
     gene            20801..21220
                     /locus_tag="AC16O9_12000"
     CDS             20801..21220
                     /locus_tag="AC16O9_12000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6517375.1"
                     /translation="MSLKKPSKRQLQALLKRARFVRDYGMRLVSVDDHECTIAMPFRS
                     ALERPGGTVNGPAFMAAADCAMWLAIKRHIGIEHDAVTSELNTAFLNAARRQTVYCTA
                     RVLKTGKRIIYGTAECHDGNGRLFTHHTVTYARVSST"
     gene            21217..22350
                     /locus_tag="AC16O9_12005"
     CDS             21217..22350
                     /locus_tag="AC16O9_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517376.1"
                     /translation="MSLAEHHEQLRDAVRRYAREQLAPNAPTWDRDKHFPRQQVKQLA
                     AMGLMGVSIPERWDGAGMDSTALAIACDEIAAGDGATCTIVMVNNLVANILQGYGSDA
                     QKESFLRPVAKGAMLGAFALTEPQGGSDAAAITTRAEKSGAHYVLNGVKQFITSGKTA
                     DLAIVFAVTDKAGGKKGISAFVVPTATPGYQVARIEDKTGQRASDTAQIVLENCKVPA
                     ANRLADEGMGYRIALANLESGRVNVAAQATGMARTALEAALAYARERKSMGKTLFEHQ
                     AVNFRLADMATQVEAARQMYLYAARLRDAGEPCLKEASMAKLFASEIAEKVCSDAMQI
                     HGGYGYVADFPVERIWRDVRVCKIYEGASDIQRLVIGRALGGG"
     gene            22350..23936
                     /locus_tag="AC16O9_12010"
     CDS             22350..23936
                     /locus_tag="AC16O9_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6517377.1"
                     /translation="MPAIRSHLNPRSEEFRANAERMRGLVADLREKVALAAAGGPEEA
                     RAKHAARGKLLPRERVRTLLDPGAPFLEVGQLAAYGMYGGDVHSASIICGVGRVSGRE
                     CVIVANDATIKGGTYYPMTVKKHLRAQEIAAQNRLPCIYLVDSGGGFLPEQDNVFPDR
                     EHFGRIFFNIANMSAAGIPQIAAVMGSCTAGGAYVPAMSDESIIVKGQGTIFLGGPPL
                     VKAATGEVVTPEELGGADVHTKVSGVSDHYAQNDAHALAICRRIVAGLNTRKQVSLDV
                     RAPREPLYPAEEIYGVIPAEVRRPYEVREVIARIVDASELDEFKQNYGTTLVTGFAHL
                     HGYPVAILANNGILYSESSLKAAHFIELAAQRGIPLLFLQNIAGYMVGKKYEAGGIAK
                     DGAKMVTAVATAQVPKFTLILGGSFGAGNYGMCGRAYAPRFLWMWPNARISIMGGEQA
                     ASVLATVRGEFKSKAEEDAFKNPIREQYERQGHPYYSSARLWDDGVVDPADTRRLLAL
                     ALSASLNAPIRPTKFGVFRM"
     gene            24024..24797
                     /locus_tag="AC16O9_12015"
     CDS             24024..24797
                     /locus_tag="AC16O9_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6517378.1"
                     /translation="MLKVETRDGVARLMLDRPEIRNAFDDALIAAVTRALRELDADKE
                     VRAVVLGGNGPAFCAGGDLNWMKRMSGYTYEQNLADAKGLAGMLQTLDRMKKPTLARV
                     HGPAYAGGIGLVAACDIAVGVPEAKFCLSEAKIGLSPATISPYVVRAMGERMARRYFL
                     SAEVFDGIEAHRIGLLSHLSASEKLDAEIDEILKNLLAGGPESLAKIKDLIRFVASRQ
                     VDDAMIADTAKRIAEIRATPEGKEGVASFLEKRRPAWKR"
     gene            24797..>25007
                     /locus_tag="AC16O9_12020"
     CDS             24797..>25007
                     /locus_tag="AC16O9_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR6517379.1"
                     /translation="MFRKILIANRGEIACRVAATARRLGIGVAAVYSEADAGARHVKL
                     ADEAYLLGPAPAAESYLRGERVIEAA"
BASE COUNT         3991 a         8610 c         8638 g         3768 t
ORIGIN      
        1 atgccgcgcc ggcgcgtcgg cggcagattg gtgttgccga tgcccgcgga gaacggatcg
       61 aggtggatct cgtccgtgat gtcggcgtga aatagcgtgg cgcgcacgga ggcggactgc
      121 tcgcgccact cggcgccggc ctccaccgtg cgcgcatgct gcgggcgcag gatctggaac
      181 tcggcgtcga acgccgcgtc gctctcgtag atctcctcgg cgttcacgaa gcggtagcta
      241 cgcccagcgc gcgcgaacca cgtccaaacc ggcgacaagg cctggcgcca gccggcctcc
      301 caggcatgct ggcgctgcgt ttcgctgact gccggcgcct gcgagccgga gcccgggtat
      361 ccgggagagg tggggtcgaa cgcgtcgctc gcgcggtact tcacacgctc ttcgcgccag
      421 ccgagcgtga agaggctctc cgcggagagc tgcatggcgt cctgcacgta gagcgccacg
      481 gcgtcctggt tcacgcgcac gcggttcacc ggctgcccga ggttctcggg ccggtccgcg
      541 cggcgcgagt cgtagcgcca ggcgttggcg tcggcgccga gcgtgaggcg gtggcgcacg
      601 ctggcaagct gcagcggaaa gcgcgcgcgc gggccgattg agccccattg cagacgctcg
      661 tcgcgcgcca cgggcccctg ctggtcgaag tacgagcgct gttccttgtc gcgccagtcg
      721 agccccagcg tgagctcggc ttcgcccacg cgatgggcga gggtcgcgcc ggcgcgatta
      781 ccgtcgcgct cggaccagtc gagcggcgtc tgcgcgccgc gcgggtcggt cgcgtactcg
      841 ttcaggccga tggaaggccg aacggagcgg gcgccgggca agcgcatctc ctggcggtcc
      901 atgccgaagc gcaagtcggc gtagctctcg cccaggcccc agcgcatgtt ggtcgccagg
      961 ttctgctgcc ggttgcggtt gttgcgccgg tagccgtcgg catcgtagcc gtagatcgag
     1021 gcgttgatgc cgaagctttc acccgcgcgc gcgccgtaga gctggccctc agccgtgccg
     1081 tagctcgcga tgcggccgag ggcttcggcg cgcgggccct gcttgagggg cgagcgtgtg
     1141 acgatgttca cgacgccggc cgtagcgcca tcgccgtaca gcacggcgcc cgtgccgcgc
     1201 aggatctcga tgcgctcgat ggtcgctagc gggatcgccg cccattgcac cccggagagg
     1261 tcgaagtcgc tgatgcgccg gccgtcgacc agcaccaggg tgttttgcgc cccggtgatg
     1321 ccgaagccgc gcagatcgat cgcagtcccg gcggcattgt tgccgaaaaa atcgcgcatg
     1381 ttgatcccgg gctgctccgc gagcagctcc ggcagcgtgc gcgccgcgct cctggcgatg
     1441 gccgccgcgt ccagtaccgt cacactagcg ggcagacggc gcacatcctc cggaaagcgt
     1501 ggagcggtga tgacgacggc gtcgccggct tgtccggccg cggcgggcgc gagcgcagca
     1561 agcagtgcga agatggcgaa acgcatggcc aaacctcccg gcgtcatccc cgacgcccac
     1621 aaagcggctg agcaacgcca caaggccggt ctccggactc gcgaggtcgc gtcgcacgac
     1681 gcgcggacgt gccgccttcc catggaacgg gttccatagt ggcggggtgg cacgcccccc
     1741 ttcgcttacc gttgcggggg cagtacaggg attgcagtcc gcgcacgcgc agactgcgca
     1801 cctgtttccc gtttcatcct cgcagagcga atccccgcga ggacaccttg cagctgactg
     1861 cgcggcggat tatacaaacc gccatgacgc gcacaatgct cggcgccctc atgctggcgg
     1921 cctgcgcggc gcaggccgag gtgcgcgtga cggacgacta cggcaagccc gtggtgctcg
     1981 cggcccccgc gcgccgcgtg gtcagcctcg cgccgcatct gacggagctg atgtacgccg
     2041 cgggcgccgg cgagcgcatg gtcggcgcgg tccaatacag cgactaccct gacgacgcga
     2101 agcacctgcc gcgcatcggc agcgaggcgt ccatcgatct cgaggcggtg gtggcgctcg
     2161 ggcccgacct ggtggtcgcc tggccgaatg ccggcagcgc gcgcgcggtg gagcgcatcg
     2221 cggcactcgg catcccggtg ttccgctccg agccgcgcga gctggaggac atcgcgcgca
     2281 cgctggagat cctcggccgg ctggccggta cggaaaccgc agcccggccg gcggcggccg
     2341 cgttccgtgc gcggaccgcg acgatcgcgc atcgccacgc ctcgcgcccg cccgtgcgcg
     2401 tgttctacca ggtctgggac cggccgctca tcaccgtgaa cggcgatcac gtcatcagca
     2461 aggtgatgcg gctgtgcggc ggcgaaaacg tcatggccga cctgcccgcc ctcgccccgg
     2521 agatcgaccg cgagctcgta ctgcgcgccg atcccgaggc gatcgtggcg agcggcgccg
     2581 acggcgcccg tccgccgtgg ctggacgcct ggcgggcttt cggggggctg gccgcggtgc
     2641 gcggggacaa cctgtacgcc atccggcccg aattgctgca gcggcatacc ccccggctcc
     2701 tggacggcgc tgacgaactt tgtcgaattc tggaggcggt ccgcaggcgc cgggcgctct
     2761 aaagccttct caggaaacac attcagaacc gccccttatt tcttgacgca gtagcggttt
     2821 tcgaacatag tgtgccgcgt ggaggcggag ctcaactcgc ttgaggcgaa ggtcgcgcag
     2881 ttcgtcggcg tgtgcgagcg cctgcgcgcc gagaacatcg ccctgcgcca ggagctcgcg
     2941 accgcccgca acgacgccaa gcggctgaac gagaagatcg agggtgcgcg cagcaaggtg
     3001 gagcagctgc tcacgcgcct gcccgagtga gcgccaacga gaagcgcgcg gccgacggcg
     3061 cgaaaaccat cgaagtcacg ctgctcgggc gcggctaccg cgtcgcctgc agcgacgagg
     3121 agcgcgaagc cctgctgcag gcggtggcct acctcgaagg caagatgaac gagatcaagc
     3181 aggcaggcaa agtcgcgggc accgagcgca tcgccgtgat ggcggcgctc aacatcgcgc
     3241 acgaactctt gagcatgcgc ctcggcgaag gctttgacat gggggaggcc aagcgtagaa
     3301 tctccctgat cgaggcgagg ctggaagcgg cgctcgccaa gcaggagaag ctgttctagc
     3361 ttcgcagtcg ttcgatgtcc cctgctgtgt tcgaccgggc tcatatttcc gaaccgatag
     3421 cttccctagg ttgcagaccc tagtgtcact gttgtgatcg tcccgccttc gggcgccgga
     3481 cgaacctgag gtgaccggaa agcgaccacc ctgatcaagc acagggttcg ggagcgagcc
     3541 caccggcaca agcggggggc taccccctca agaagaacta gaatttcccg aacatgccct
     3601 tctggctgat gaaatccgag cccgacgagt gctcgatcga cgacgcgctg gctgcgcccg
     3661 ggcgcatcac cccgtggacc ggcgtgcgca actaccaggc gcgcaacttc atgcgcgacg
     3721 cgatgcgcgt cggcgacggc gtgttcttct accactcgag ctgccctgag cccggcatcg
     3781 cggggctggc cgaagtcgcg tcggcgccgt acccggatcc gacccagttc gaccggcgca
     3841 ccgactacta cgacgcaaaa tcgaaacgca cctcgccccg ctgggccatg gtcgatgtgc
     3901 gtgcgctgaa gaagatcccc ctcgtgccgc tcgcggagct gcggcggcac aaggcgctcg
     3961 cgaagatgtg gctgctgcgt ccgggctcgc gcctgtccat cacgccggtg agccaggtcg
     4021 agtggcgcta catcaccgga aagctccttt gacgaacgac acgctgctgc gcgcgctggc
     4081 gtgcgagccc acggcctaca cgccggtgtg gttgatgcgc caggccgggc gctacctgcc
     4141 cgaataccgc gccacgcgcg agcgcgccgg cagcttcctc gcgctggcga aatcgccggc
     4201 gctcgccacc gaggtgacgc tgcagccgct ggagcgcttt gcgctcgatg cggcaatcct
     4261 gttctccgac atcctgaccg tgccggacgc gatggggctc gggctgcatt tcgccgaggg
     4321 cgagggcccg cgcttcgcgc gcccgctgcg cgacgaagcc gccatccgcg cgctcgccgc
     4381 ccccgatcct tccgatcggc tgcgctacgt gatcgacgcg gtgcgcgaga tccgcaaggc
     4441 gctcgccggc cgcgtgccgc tcatcggctt cgccggcagc cctttcaccc tcgcttgcta
     4501 catggtcgag ggcgcgggca gcgacgattg gcgcgcgctc aagacgatgc tctatgcgcg
     4561 gcctgacctg ctgcaccgca tcctcgaggt gaacacgcgc gccgttaccg attacctcaa
     4621 tgcgcagatc gaggccggcg cgcaagtggt gatgctcttc gacacctggg gcggccagct
     4681 gagccacgcc ggatatgagg cgttctcgct cgcctacgcg cggcgcattt tcgccgcgct
     4741 caagcgcgaa agcggcgggc gcaaggtgcc tgcgattctg ttcaccaagg gcggcggcgc
     4801 gtggctggag gcgatggcat cgagcggctg cgacgcgctc ggtctcgatt ggacggtgga
     4861 tccagcggcg gcgcgtgcgc gcgtcggtcc acgggtcgcg ttgcagggaa atctcgatcc
     4921 cgcggcgctc ttcgcgccgc ccgaagcggt gcgtgcagag gcgcggcgcg ttctggacgc
     4981 gttcggcgcg gcgcccggcc acgtcttcaa cctgggtcac ggcatctccc agcacacgcc
     5041 gatagaaagc gttgccgcgc tcgtggacga agtgcgcact tacagcagca agttgcgtcc
     5101 cgccacaaaa tgaaacgctt gacttatgca caaagccgct ttcttcctct atgcagcgga
     5161 cgtttttcga gattccccct ggagagcccg gcaacccatt gatttcatgc agtaaaacac
     5221 cgcgtctcgc cctgggcgct gcggcgtggc tcgtgcgggc tcggggcttg acggaaacgt
     5281 gaccggctta ttcacattgt tatccacacc cttggcgggc aagggaaaat gccttttcgc
     5341 agcgaggctt acacgctcgc gcgctcgccg atgaccgtcc tgcgcgttgc gctcgacgtg
     5401 ccgctcgcca cgctttttga ctacgcgcct gccgacgaag gcgtcgcacg cgtcggcgat
     5461 cgcgtggtcg tgcctttcgg cacgcgcgcg cgcgtcggtg tggtcgtgga ggagggcacg
     5521 agcagcacga tcgcagccgc gaagctgaag cgtgtagcgc gcgtactcga cgacgcgccg
     5581 cgcttgcccg cgccgtggct ggagctgatg cgctttctgg ccgcctacta ccagcgtccg
     5641 ctgggcgaga ccgtgatcgg cgcgctgccc ccgcgcctgc ggtcgctgaa accgctgccg
     5701 aagaaggcgc gcgcgccggc tgcggctgac ccggcgtcgc cgcatttcgt ctccccgcac
     5761 caaccgaccg ccgcccaggc gcaggccatc gagcgcatcg gcggcgcgct cggcgggttc
     5821 aaggcgttcc tgctgcacgg cgtgaccggg agcggcaaga ccgaggtcta cctgcgcctc
     5881 atctcgcagg tgctggagcg cggcggacag gcgctggtca tggtgcccga gatcagtctc
     5941 acgccgcagc tcgaggcgcg ctttcgccgc gcctttccgg gggcccgcgt tgcacttctg
     6001 catagcgcgc tcgaggacgt gccgcgcacg gcggcctggc tcgccgcggc gcgcggcgag
     6061 gccgggctcg tgctcggcac ccggctcgcc gtactcacac cgctgccgaa cctcgccctg
     6121 gtcgtggtgg acgaggagca cgacacgtcg ttcaagcagc aggaaggatt acgctattcc
     6181 ggccgcgacg cggcggtggt gcgggcgaag cacgccgcct gccccgtcgt cctcggcacg
     6241 gcgacgccgt cgctcgagac ctggcagaac gcaatcgaag gccgctacga gcggctcgcg
     6301 ctgcccgaac gcgccgtgcc gggggcgcgc ctgcctgcgg tacgcacgat cgacctgcgc
     6361 cgcgaaggcg ccgagcacgg cttcgcgcca agcctgttcg cggcgatcgg cgagcgcctg
     6421 ccgcgcggcg agcaaagcct tgtcttcatc aaccggcgcg gctatgcgcc ggtgctcgcc
     6481 tgccacgcct gcgactggac cgcaggctgc aagcggtgca cggcgcgcat ggtgctgcac
     6541 cgcgtcggcc actgcctgcg ctgccatcac tgcggcgacg aggcgccggt gccgcgcgcc
     6601 tgccccacct gcggcaacct cgacctgaag gcgatgggcc gcggcacgca gcgcgtcgag
     6661 gacacgctcg gcgcccgctt tcccggtgcg cgcatcgtgc gcatcgaccg cgacagcgcg
     6721 cgccggcgca gcgagctctc gcgcaccctg gagggcatcg cgcgcggcga ggccgacatc
     6781 ctggtgggca cgcagctcct ggccaagggc cacgacttcc cgctgctcac gctggtgggc
     6841 gtgctcgacg cggacaacgc gctcgtgtcc accgattacc gcgccgccga gcgcctgtac
     6901 tcgaccctcg cgcaggtcgc ggggcgcgcg ggacgcgccc ggcacgcggg cgaggttctg
     6961 gtgcagacgc gctatccgca gcatccgctc tatgcggcgc tcgctcgcca cgactacgcc
     7021 accttcgcag ccgcgcaact cgccgagcgg gagagtgccg ggtttccgcc ctttgtgcac
     7081 gaggcggcgc tgcgcgccga ggcacccaag ctcgcgctgg cgctgcaatt cctcgaggac
     7141 gcggcgcggc tcgtgccggt gccgcaagag gtgcgcgtct acgatccggt gccgcacgtg
     7201 atcacgcggc gcgccgacct cgagcgtgcg cagcttctcg tgcagtcggc ctcgcgccct
     7261 gcgctgcagg gtttcctcgc cgcctggacc gagcggctgt tcgccgcggc gccgcgcggc
     7321 gtacgctggc acctcgacgt ggatcccatc gagttcagct aaatggggac agaccccatt
     7381 tataattcgc gccctttcat ggacatcaaa gcccacctcg cgacgctcct tcgcgcagcg
     7441 ctagtgcgcg cggccccgga ggcggcggac gcgcaggtgc tgatcgagcg gccgcgcgac
     7501 gtggcgcatg gcgatttcgc ctgcaacgtc gcgctgcagc tcgccaagcg cctgaagcgc
     7561 aagccgcgcg agctcgcgga gcggctgacg gccgcggttg ccgcggaccc ttacgtggcg
     7621 aaactcgagg tcgcgggcgc gggcttcatc aacatccgcc tcgcgccgcg cgccgggcag
     7681 gaagcagtgg cgcgcgtcct ccaacttggc gcggcgtacg ggcatgcgcg cgcggcgcag
     7741 gcccccaaga cgatggtcga gttcgtgtcg gcgaatccca ccggaccgtt gcacgtgggg
     7801 catggccgcc aggcggcgct cggcgatgcg ctcgcagcgc tcctcgcctc gcagggccac
     7861 gacgtgacac gcgagttcta ctacaacgac gccggcgcgc agatccacaa cctcgcgctg
     7921 tcggtgcagg cccgcgcccg cggcaccaag cccggggacc ccggctggcc ggccgacggc
     7981 taccgcggcg agtacatcga ggaaatcgcg cgcagtttca cggcggcagg cggcgatgtc
     8041 ggcgacgtgg aggccgtgcg cacgttcgcg gttgcggcgc tgcgccgcga gcaggatgcc
     8101 gacctgcagg ccttcggggt caagttcgac gtgtactacc tggaatccag cctttacacc
     8161 gacggccgcg tggacgcggt ggtgaaggcc ctggtcgcaa gcggcaagac cttcgagcag
     8221 gagggggcgc tgtggctgcg caccacggac tacggcgacg acaaggaccg cgtggtacgc
     8281 aaatccgacg gcagctacac ctatttcgtg ccggacgtcg cgtatcacgt ggcgaagtgg
     8341 gaacgcggct tcgccaaagt gatcaatgta caaggcaccg accaccacag taccgtcacc
     8401 cgcgtgcgcg ccggcctgca agcgctcagc ctcggcatcc cgtcgggcta ccccgactac
     8461 gtgctgcaca acctggtgcg cgtgatccgc ggcggtgagg aggtgaagat ctccaagcgc
     8521 gccggcagct acgtgacgct gcgggacctg atcgactggg tgggccgcga cgcggtgcgg
     8581 ttcttcctcg tctcgcggcg cgccgactcg gacttcgtct tcgacgtcga cctggcgcgc
     8641 tcgcagtccg aggagaatcc cgtgtactac gtgcagtacg cgcatgcccg agtttgcagc
     8701 gtgttctcgc aatggggcgg ggacgccgcg ccgctccccg gcgcgccgct gggcgccctg
     8761 acaggcgggc gcgaacaggc gctgatgcgc cgcctgatgg agtttccgga gatgctggcg
     8821 gatgcggcgc acgagctcgt gccgcacgcc atcgcctttt acctgcgcga actcgcagga
     8881 gagttccaca gctactataa tgccgagcgc attctggtcg aggatgacgc cacgcgcacc
     8941 gcgcgcctcg cgctgtgcgc cgcagtccgc caggtgctcg ccaacgggct cgcactgctc
     9001 ggcgtacgcg ccccggaaag gatgtgatgg cggcaatacc acacgcgacg gcacgcaagg
     9061 gatcgggcgg cggcttcctc gtcgggatgt tcgtcggcct gctcttgggc ctgggcatcg
     9121 cgctcgggat tgcgctttac ctgaacaaga cgccgatccc gttcatgagc cgcgccaagc
     9181 cgctggccga ggacggcaag ggcgaggccg gcaaaccgtc cgcgctcgcc gggctgccgc
     9241 aggggggcga caagcccgca gcgcaggtgc cggtgaaacc gcccgagaag ccgcgcttcg
     9301 acttctacaa gatcctggcg ggcggcgaag agcccctcag cgaccgcgag ctgcgcgagc
     9361 gcctgaaggc ggcgaagacg cctgagccgg cgcagactgc ggccgtcgcg cccgcggaag
     9421 gcaaggacat ctatttcatc caggcgggcg cgttccagaa tccggccgac gccgacaacc
     9481 agaaggcgcg cctggcgatg ctcggcttcg agtcgaacgt cgagccgacc aacctgcccg
     9541 acaagggcat gttctatcgc gtgcgcctcg gcccctacgc caaggtcgag gagctgaacc
     9601 gggtgcgcca gtcgctggcg cagaacggaa tcgacgccaa cctggtcaaa ctgaaggacc
     9661 ccgccacccg gaaggtgaac tgaatggatt tccgccgccg caccctcgtc gctgccctgg
     9721 ggcttgcccc gcttgccggt ctggcccagg cgcgccgtcc gacgcccggt gtcgatttct
     9781 ccgagctgcg acccgcgcag cccgtggagg cccagggcaa gatcgaggtc atcgagttct
     9841 tctggtacgg ctgcccgcac tgctattcgc tgcagccatt gatcgaaaaa tgggttccac
     9901 gcctggccgc ggacgtgaat ttccggcgcg tgcctgcggt gctgaacgaa ggctgggcgc
     9961 gtgaggcggc ggtgttctac gccttcgagg cgctgggggt gctggaaaag ctgcaccggc
    10021 cgttcttcga cgccatccat gtcgatcgca tcagcacgcg caaccccgcc acgctcgcgg
    10081 agtggctgcg taagcaggag gtcgacgtgc agaaatacga ggatgcgttc aagtccttcg
    10141 gcgtgcagag caaggtgcgg cgcgccgcgc agctttccgt cgcctatcgc atcgacggca
    10201 cgcccgcgct cgcggtgcac ggacgctaca cggtgagcgc ggagcaggga cgcacccagg
    10261 ccggcatgct gagcaccgcg gattacctga tcggcgtcgc gcgcaagggc gcctgagccg
    10321 ctactcgcgc atcgtcatca cgggcgcctc cgacgggctg ggcgccgcgc tggcacggca
    10381 ttacgcgtca cccgacacgg tcattggcct gatcgcgcgg cgtggcgagc cgatgcaggc
    10441 tctcgccgcc ggcttgcccg gtagattcga gatttacgcg ctcgacgtgg ccgaccacgg
    10501 cgcgctcgag cgcgcggcga aggagttctg cgcgcgccat ggcgcgcccg acctcgtcgt
    10561 cgcgaacgcc ggcctgtcgg taggcacgcg cggcgaggac agcgccgacc tcgtgaagct
    10621 gcggcgcctg ctggaggtca atgtgaccgg gcttgcggcc acgctcgctg ccttcgcccc
    10681 ggcgatgcgc gccgcgcgcg ccggcacgct gtgcggcatc gcgagcgtag cggcgttccg
    10741 cggactggcc ggcgccggcg cctacagcgc gtcaaagtcg gcggccatcg cctggctcga
    10801 ggcgctgcgg gtcgagatgg cgggaagcgg tgtgtgcgtc gtcacgatct gccccggcta
    10861 catcgatacg ccgatgacgc gggtgaaccg ctaccgcatg ccctttctcc tgtcggcgga
    10921 cgaggccgcg cggcgcatcg cgcgcgcgat ccgcgcgcgc cggcgcctcg ccgtgatccc
    10981 gtggcagatg gccctggtgt cggtcgtcct gcgcgcgctc cccggctggc tctacgatcg
    11041 ccttttcgcg cgcgggccgc gcaagccgcg cgacctgccg ctttgacgcg catcgtttcg
    11101 ctcgtaccgt cgctcacaga gctggtgtgc gcgctgggcc tcggcgcggc gctggtcggc
    11161 cgcaccggct tctgcgtcca cccgaaagac gtggtgcgcc gcatcccgaa ggtcggcggc
    11221 accaaggatg tcaacctcga caaggtgcgc gcgctcgcgc cgacgcacgt ggtgctcaat
    11281 gtggacgaga accgcaagga gaccgcggac gagctggcga cgttcgtgcc gaacctcgtc
    11341 gtcacgcacc cgctcggccc gctcgacaac ctcgccctct accgccagtt cggccgcacc
    11401 ttcgcccgcg agcgcgaggc cgaggcgctg tgcgcgcggt tccaggcggc ctatgatgaa
    11461 gcccgcgggc tgcgcctgcc tgagcggcgc gcgctctatc tgatctggaa agatccctgg
    11521 atgacggtgt cgcgcgacac ctacatctcc cgcacgctgg cgatctttgg cctgaatacc
    11581 gtgcccgaag cagccgcgca gcgctacccg aagctcgagg atctcgccag cgtggacgcc
    11641 gacctcgtgc tgctttcctc cgagccctac cgtttccgcg accggcacgt ggcgcaagtt
    11701 gctggaatca ctggaaaacc cgcgctgctg gtcgacggcg agatgacctc ctggtacggg
    11761 ccgcgcgcca tcgccgggct ggattacatg gcgaaattcg cagcgcacgc ctagcaacgc
    11821 tcggcgtatg ctgaggtggc ggtgtcgccg atcggcgccg cgtcacctgg gagaggggat
    11881 tatggacgga acgcggatat tgaagcgctc ggcgctggcc ggtgtcgctt cgctgctgtg
    11941 catgtacgga accgccagct gggcggtgaa cgacgagccg ctgaaggaca aatgggcgcc
    12001 gagcgagttc ggtaaggacg acaaggcggg tgcggccaac cggcgcaccg ccgcatcggt
    12061 gctcaaggcg gcgaaactcg tgaagaaggg caaggtcgcg acgctgggca aggtgtacca
    12121 gcaggacgcg ccggggttcg gctcgcgcgg ctggcgcttg gtgattcccg gcctgccgac
    12181 cggcggcccg ttcggcaagc aggaactcct gtacaacgac gagtacgtcg cgaccgagat
    12241 cgggcagatc ggcacccagt tcgatggccc gggccacatc ggggtgcaca cctcgaaggg
    12301 catgttcttc tacaacggcc gctacctgca cgacgaaggt gtaagcacct acggcatggg
    12361 gccgcttggc gtcgagcacg tcggccagct cggctacgcc tgccgcggcg tgctgctcga
    12421 tgcggtggcg ctacgcggcg gcaagcagct gccgatcccg gccaaggccg gcgacccggg
    12481 catcatcacc gacaaggacg tggaggcgat ggtcaagcgc cagggcatcg cgccgatcgg
    12541 cgagggcgac tgtgtgttcc tccacaccgg ccacggcgac atctggcatc cgagcaagtg
    12601 ggacagcttc agcgcggagg aaaagcagaa gcgcatcgcg cacttcaatt cgggcgagcc
    12661 gggcttcggc atctcggcgt gcgagtacct cgccaagcgc aaagtcgcgc tggtgggcgc
    12721 cgacgtctgg gcggtggaag ccgtccccgg cgagggcgag cagcccttcg aatgccacat
    12781 caagatgcag accaagcgcg gcatctggtt cctggagaac atggacctga gccagctcgt
    12841 ggctgacaag gcctacgagt tcctgttcgc ctggtcgccg ctgaagatca agggcggcac
    12901 cggctcgccg ggcaaccccg tcgcaatcta ctgaggcggc agggagatca cggcgcgagc
    12961 atcgcgcgcc ggatctcctc ctgctcgggg aagtcgcgct cgtcgcgggg cacccgcccc
    13021 gcgtgcgcgc gcacgcggta gcagtccggg tcgtagggcg tctgcggctc gaggccctcg
    13081 gcgagccggc gcgccgcgcc gagcaggcgc tggcgcagcg cgacgaccgc cttgtcgctg
    13141 gtgccaaggt gctcgcgcgt gcgatcggcg accgcgcccg ccgataccgc aagtccgaga
    13201 tgcgccgcat cggcgcgctg ccatagctcg cgctggcgcg cgctcgcggg cccgtcggtg
    13261 cgccagttca cgctgagcac cgaggtgcgc tcgtcgtcct cgggcaccca agcgcggaac
    13321 agccgcgaat cgggatcgtc cgcgggcggg acgagcgtga agcagggcat caggaacaag
    13381 ctgatgcgcc agagatgctg ctcgccctgg accgagcggc gcacgccgca ggcgaggcca
    13441 tagggcgcct cgtgcaccgt gtaacgcggt gccggatcgt ccgccccggc caggccgaag
    13501 cgagactcgt cgatctcccc ctcgagtgcc tgggcgtagt tgcactcata aacccggcgt
    13561 gacgcgttgc gttgcgccgc cggcaggccc agccattcga gctcgggcag ctcggcgcgc
    13621 ttctcggcgg gacccagata ggcccatagc agcccggcgc gctcggttac cgggtaagcc
    13681 ttggctctta ccttgtcgcg gaacgccgag cccgcgggtt ctgagggcat ctcgaggcag
    13741 cgtcccgaga cgtcgaactt ccagccgtgg taggcgcagc gcaggcctcc cgcctcgttg
    13801 cgtcccaaac acaggctcgc gcgccgatgc gggcaattct cgtcgaggag cccggggcgg
    13861 ccgcggttgt cgcgaaatgc caccagggac tcaccgagca ggcgaacgcg ctgcggcgct
    13921 gactcgagta ccagctcatc ggccagcatg aagggcagcc agtggcggcg gaacagcgcc
    13981 cccatcaccg tacccggccc cacgcgcgtc aggagcgcgt tgtcttctcg gctcagcatc
    14041 taatagggga cagacctcat ttatctctgc acgagaaaaa tgaggtctgt ccccattttc
    14101 attttctgct acttcttctt gctcaggcgc tgcatgacgt aggagccggg ggcgtcctcg
    14161 atcgctttcg acgggatgcg cgccggaacc ttgtcgccgc ggttctgctg cgcgatccag
    14221 cgctgccagt cctcccacca ggagcccggg tgctgcgtcg cgccctcgaa ccacgcgtcg
    14281 ggcgtatcgg cgaagctgtc gttggtccag tagtggtact tcttcgccgc cggcgggttg
    14341 acgatcccgg cgatatggcc cgagccgccg agcacgaagc gcacctcgct cccgaggtac
    14401 ttcgcgccct cgtaggtggt cttccacggc gcgatgtggt cttcgaccgt ggagatgaag
    14461 tacgcgggca ccttgacctt ggaaaggtcg atcggctccc cggcgagcgt cacggcgccc
    14521 ggcacgccga gcttgttctt caggtacatg ttccggaggt agaagctgtg catgcgcgcg
    14581 ggcatgcgcg tcgagtccga gttccagtac agcagatcga acggaaaagg gtccttgccc
    14641 atcaggtagt tgttgatgac gaaggaccac accaggtcgt tcgagcgcag catgttgaag
    14701 gtggtcgcca tctccgagcc ctcgaggtag ccgcgctcgt tcatgcgccg ctcgaggttg
    14761 tcgacttgcg cctcgtcgat gaacacgccg agctcgcccg gctgcgaaaa gtcgagcagc
    14821 gagacgaaga acgtggcgca gccgacgcgc gcgtcgcctt tcccggcgag gtaggcgagg
    14881 gcgcagccga ggagcgtccc gcccaggcag tagccgatga cgttcaggtg cttctcgccc
    14941 gtggcccgca tcacggcgtc catcgccgcc agcgcgccct cggtgagata gtcgtcgaag
    15001 cccttctggg cgagtttcgc gtccgggttc acccacgaca cgacgaacag cgtgtgtccc
    15061 tggtccaccg cccacttgat gaagctgttc ttttcgcgca ggtcgaggat gtagtacttg
    15121 ttgatccacg gcgggatgac gagcagcggc cggcggaaca cgtccggggt cgacggctgg
    15181 tactggatga gctgcagcag cgcgttctga aacaccacct tgcccggcgt ggtggcgacg
    15241 ttcctgccaa gctggaacgc gtcctcgtcg gtcatgctga tgcgcagctg cccctcgccc
    15301 ttctcgatgt cggcgagcag gttgttcagg cccttcacca ggttctggcc gcccgaggcg
    15361 accgtctcgc gcagcacctg cgggttggtg gcggcgaagt tcgagggcgc gagcgcgtcc
    15421 acgtactggc gggtgaagaa ggcgatcttc ttctgcgact cctcgggcag cccctgcact
    15481 cccgccaccg cgtgctgcgt gtggcgagcg gcgatgagat acgactgctt gatgaagtcg
    15541 aagaggaagt tgtccgtcca ctcctggtcc ttgaagcgca cgtcgccctt ggccggctgt
    15601 gccaccggcg cgggcgcctg gcccatcatc ttcagccacg acgactgcca caggcgcatc
    15661 gaatcgatcc agaaattgac cgacgccgcg gcgagcgcgc tcgggtccat cagcaggcgc
    15721 gagtacagat ccatgtacgc cctggcgatg ccgagttcgt ccttcaccgc cgaggagatc
    15781 gtgctctgca gctgcttttg cccgaattcc ttgaggatgc gcgaggagcg ttcggccacc
    15841 tcgtgcaaca ccttcgcggt ctcctcgcgg tttatgccgt cagcgctttc tcccatgctt
    15901 tctccttgat gagggcgaac cggctcacgc cgtcctcgcc gcgcatgcgg cgcaggcgtc
    15961 cggcgcgctc caggtaatgc aggtgagcga tcgcctcgcc catggcgaag aacatctgct
    16021 gggtgtcgag cggccggcga aaaatgagcg ccagcacgtc ggcggcgctg cgcggggctt
    16081 cgccgcaggc cgtgagcagc tccgccaggc gcgtctcgtg gtgctcttcc agctgcgcca
    16141 cgcgcgcttg cgcgccgcga aatggcagcc cgtgcgaggg cagcaccagc acgtcctgcg
    16201 gcagctcgcg atagcggcgg atggagcgca ggaagtaacc cagcggatcg ccctcggggt
    16261 ccgtcggcca gacgccgacg tgcgtggaga tcttcggcag cagcatgtcg ccggagatga
    16321 ccacgttgag cgcctcgcag tagagcgagg cgtgctcggg cgcgtggccg tagcccgtga
    16381 tgacgcgcca ggcgcgcccg cccacgccga tcgactcgcc ctcgaggatg cgacggtact
    16441 gcgtcggcag ctcgcccacg aggcgccggt actggttgcc gcgcatcccc agctgcccga
    16501 gccgagcctc gtcgagcccg tgcgcgcgga atagccccag cgtcgcctcc gccacgaatc
    16561 ccgcggcgcc cgcgcgcacc gcgtgcgcgg tcaggtattc gccctgcgtg atccagagcg
    16621 gcgcgccgaa gcgctcgcac agccagccgg cgttgcccgc gtgatccggg tggaaatgcg
    16681 tgacgaggac ccgccgcacc gcgcgcgcgc cggtggcggc gaacacctgc tcccattgcg
    16741 cgcgcgtcgg cgcgtcggcg aggccggtgt cgaccagcgt ccagccgtcg ccgtcctcga
    16801 gcatccacag attgatgtgg tccagcgcga acggcagccg catgcgcagc cagcgcacgc
    16861 cgggcgcgat ctcgcgcacg gcgccgggcg ccggcgcttc cggatgggga aattcgaggg
    16921 cggacacggc ggcaagctgg gctagaatca actgcaccct accatgcggc aaatcgaaaa
    16981 ggaacgcgcc gatgccgagt actcgatcgg cgagctcgcc gccgagttcg acgtcacgcc
    17041 gcgcgcgatt cgcttttacg aggccgaagg cctgctcgcg ccgcggcgcg aaggccagcg
    17101 acgcatctac acgccgcgcg agcgcacgcg cctgaagctc accttgcgcg gcaagcgcct
    17161 ggggctgacg ctttccgaga tccgtgatct catcgacatg tacgagcccg gacaggacca
    17221 gcggccgcag ctcgagcgct ttcttgcggt gctcgagacg cacaaggcgg cgctcgagcg
    17281 ccagcgcgag gacatcgccg tgcagctcgc cgagatcgcc gccttcgaga agcgcgtgcg
    17341 caagcagctc gcggcgcgcc gccgaggttg acgtttacgt aaacgtcacg tagcctcgcc
    17401 ccgatgcacg ccccgcagcc gcgcttccag ccccgggatc ccggctacgc cgcgcgcgtg
    17461 caggaaagct tcgggcgcca gaaagcgatg cagcacatcg gcgcgcggct gcgcgcggtg
    17521 ggcccgggct ggaccgaggt cgagttgccg catcgcgacg agctgaccca gcaaaagggg
    17581 ttcctccacg gcggaattat cggcatgatc gccgacaatg cctgcggtta cgcggccttc
    17641 agtctgatgc cggcgggcgc ctcacccctc accgtggagt acaagatcaa catcctcgcg
    17701 ccaggtcgcg gcgacaggta cgttgccgag ggcgtggtcc tcaggtccgg gcgcacgttg
    17761 tcggtcacgc gcgccgaggt ctacgcggag accgccgccg ggcgcgtgca cgtggcgagc
    17821 atgcagcaga cggtcctcat gctcgccgat accccggaca cgcccgaatg aatttcctgc
    17881 gcttcgagca cggcgagacg ctcgacatgc tgcgcgatac cgtgcgcgct ttcgcgcaga
    17941 aggagatcgc gccgcgcgcc gacgagatcg accgcgcgaa cgagtttccc gccgacctgt
    18001 ggaagaagtt cggcgcgctc gggctgctcg ggatcacggt cgaggaagcc tacggcggca
    18061 ccatgatggg ctatctcgcg cacatcatcg ccatggagga gatctcgcgc gcctccgccg
    18121 cggtgggcct gtcctacggc gcgcactcga acctgtgcgt gaaccagatc cgcaggaacg
    18181 gcagcgacgc gcagaagaag aaatacctgc ccaagctcgt ctcgggcgag cacgtcggcg
    18241 cgctcgccat gagcgagccc ggcgcgggct cggacgtggt cggcatgcgg ctgcgcgccc
    18301 aaagggtgaa aagcggccgc ggcgacgcct atgtcctcaa cggcaacaag atgtggatca
    18361 ccaatggccc ggacgccgac gtgctcgtca tctacggcaa gacgagcgac cagggcatca
    18421 ccgcgtttct ggtcgagaaa ggcatgaaag ggttcagcac cgcgcaaaag ctcgacaagc
    18481 tcggcatgcg cggctcgaac acctgcgagc tggtattccg cgactgcgag gtgccggcgg
    18541 ggaacgtgct cggcgaggaa ggcaagggcg tcaaggtgct gatgagcggc ctcgattacg
    18601 agcgcgccgt gctctcgggc gggccgctcg gcgtgatggc cgcgtgcctg gacgtggtcc
    18661 tgccctacgt gcacgagaga aagcagttcg gccagccgat cggcgagttc cagttgatgc
    18721 agggcaagct cgccgacatg tacaccacgt tctccgcgtg ccgcgcctac gtctacgcag
    18781 tcggccaggc gctcgatcgc ggcgagacca cgcggaagga cgccgccggc gcgatcctct
    18841 acgccgccga gaaggcgacc tggatggcgg gcgaagcaat ccaggcgctg ggcggcatgg
    18901 gctacatcaa tgagacgccg accgggcggc tgtggcgcga cgccaagctc tacgagatcg
    18961 gcgccggcac ctccgagatc cgccgctggc tcatcgggcg cgagctgttc ggggagagca
    19021 catgaagctg cagcagctcc tcgagaacaa ccgcgcctgg gcgcgcgaag cgctcgccgc
    19081 cgacccggag tacttcgcgc gccttgcgcg ccagcagtcg ccgcagttcc tgtggatcgg
    19141 ctgctcggat tcgcgcgtcc cggcaaacca gatcaccggg ctcgcgccgg gtgaagtgtt
    19201 cgtgcaccgc aatatcgcca acgtcgtcgt gcacaccgat ctcaactgcc tgtctgtgat
    19261 ccagttcgcg gtggacttcc tcaaggtgcg gcacatcatc ctgtgcgggc actacggctg
    19321 cagcggcgtc gccgcggcga tgagccagca gcgcaccggg ctgtcggaaa actggctgcg
    19381 ccatgtgcag gatgtggccg cgaagcatgg cggcgagctg cgggcgatcc agagcgacga
    19441 cgcgcgctac cggcggctgt gcgaactgaa cgtacgcgag caagtccacc acatcagcca
    19501 gtgcacctcg gtccaggacg cctgggcgcg ccagcagccg ctcgcggtgc acggctggat
    19561 ctacgacctg caggatggtc tgctgcgcga cctcgactgc acccgaacgg agtaacgcca
    19621 tggcccacga cccgatcgtc atcgtttccg ccgcccgcac gccgatgggc ggcttccagg
    19681 gcgagctcaa gagcttcgcc gcgcccgagc tcggtgccgc cgccatccgc gccgccgtcg
    19741 agcgcgccaa ggtcaagccc gaggacgtgc aggaggtgct catgggctgc gtgctgcccg
    19801 cgggccaggg ccaggcaccg gcgcgccagg cttcgctcgg cgcgggcctg ccgctcgccg
    19861 cgggctgcac cacggtgaac aagatgtgcg gctcgggaat gaaagccgcg atgctggcga
    19921 gcgacctgct cgccgccggc agcatggaca tcgtcgtcgc cgggggcatg gagtcgatga
    19981 ccaacgcgcc ctacctcctg cccaaggcac gcgctggcct gcgcatgggc cacgggcagg
    20041 tgctcgacca catgttcttc gacgggctcg aggatgccta cgacaagggc cgcctgatgg
    20101 gaaccttcgc cgaagactgc gcgcagaagt acgctttctc gcgcgaggcg caggaccgct
    20161 ttgccatcgc ctcgctcgag cgcgcgcaga aggcgaacca ggaaggctgg tttgcctggg
    20221 agaccacgcc ccttgccgtc aaggcgggca aggaggagaa gttcatcgag accgacgagc
    20281 agcccttcaa ggcgaacctg gcgaagatcc cgacgctcaa gcccgcgttc cgcaaggacg
    20341 gcacggtgac ggcggccaac tcgagctcga tctcggacgg cgcggcggcg ctcgtgctca
    20401 tgcgccgctc gacggcggac aagcgcggcc tcgcacccat cgccaccgtc gccggccacg
    20461 ccacgcacgc gcaggagccg gggtggttca ccacggcgcc ggtgggcgcg atgcgaaagc
    20521 tcttcgagcg caccggctgg tccgcgggca aggtggatct tttcgagatc aacgaagcct
    20581 tcgccgtcgt cgccatggcg gcgatgaagg agcacgggct gccgcacgag aaggtgaacg
    20641 tgcacggcgg cgcctgcgca ctcgggcatc cgatcggcgc ctcgggcgcg agaatcctcg
    20701 tcacgctcat cggggcgctg cgcaagtacg gcggcaggcg cggcgtggcg agcctgtgca
    20761 tcggcggcgg cgaagcgacc gccatggcgc tcgagctcgc gtgagcctga aaaagccgtc
    20821 caagcgccag ttgcaggcgc tgctcaagcg cgcgcgcttc gtgcgcgatt acggcatgcg
    20881 cctcgtttcg gtggacgacc acgaatgcac cattgccatg ccgttccgca gcgcgctcga
    20941 gcgccccggc ggcacggtca acggccccgc cttcatggcc gcggccgact gcgccatgtg
    21001 gctcgccatc aagcgccata tcggcatcga gcacgacgcc gtcacgtccg agctcaatac
    21061 cgcgttcctg aacgcggcgc gccgccagac ggtctactgc acggcgcgcg tgctgaagac
    21121 cggcaagcgc atcatctacg gcaccgccga gtgccacgac ggcaacgggc gcttgttcac
    21181 gcaccacacg gtcacctatg cgcgggtgag ctcgacatga gccttgccga gcatcacgag
    21241 cagctgcgcg acgcggtgcg gcgctacgcg cgcgagcagc tcgcgcccaa cgcccccacg
    21301 tgggaccgcg acaagcattt cccgcgccag caggtgaagc agctggcggc aatgggactg
    21361 atgggcgtgt cgattcccga aagatgggac ggcgccggca tggacagcac cgcgctcgcc
    21421 atcgcctgcg acgagatcgc ggccggcgac ggcgcgacct gcaccatcgt catggtgaac
    21481 aacctggtgg cgaacatcct gcagggctac ggcagcgacg cgcaaaaaga gtccttcctg
    21541 cgcccggtcg ccaagggcgc catgctcggt gccttcgcgc tcaccgagcc gcagggcggc
    21601 tcggacgcgg cggcgattac cacgcgcgcc gagaagtccg gcgcgcacta cgtcctgaac
    21661 ggggtcaagc agttcatcac ctcgggcaag accgcggatc tcgccatcgt gttcgccgtc
    21721 accgacaagg ccggcggcaa gaaaggcatc agcgcgttcg tcgtccccac cgccacgccc
    21781 ggctatcagg tggcgcgcat cgaggacaag accggccagc gcgcttccga cacggcgcag
    21841 atcgtgctcg agaactgcaa ggtgccggcg gccaaccgcc tcgccgacga gggcatgggc
    21901 taccgcatcg cgctcgccaa cctcgagtcc ggccgcgtca acgtcgcggc gcaggctacc
    21961 ggcatggcgc gcacggcgct cgaagcggcg cttgcctacg cccgggagcg caagagcatg
    22021 ggcaagacgc tgttcgagca ccaggcggtc aacttccggc tcgccgacat ggccacgcag
    22081 gtcgaggccg cgcgccagat gtacctctac gccgcgcggt tgcgcgatgc gggcgagccc
    22141 tgcctgaagg aagcgtcgat ggcgaagctc tttgcttccg agatcgcgga aaaggtgtgc
    22201 tccgacgcca tgcagatcca cggcggctac ggctacgtag ccgactttcc cgtcgagcgc
    22261 atctggcgcg acgtgcgcgt atgcaagatc tacgaagggg cgagcgacat ccagcggctg
    22321 gtgatcgggc gcgcgctggg gggcggctga tgcccgcgat ccgcagccat ctcaacccgc
    22381 gctcggagga gttccgcgcc aatgccgagc gcatgcgggg actcgtcgcc gacctgcgcg
    22441 agaaagtcgc gctggccgcg gcgggcggcc ccgaggaagc gcgcgcgaag cacgccgcgc
    22501 gcggcaagct cctgccgcgc gagcgcgtgc gtacgctgct cgacccaggc gcgccgttcc
    22561 tggaagtggg ccagctcgcc gcctacggca tgtacggcgg cgacgtgcac tcggcctcca
    22621 tcatctgcgg cgtcggccgc gtctcgggcc gcgagtgcgt gatcgtggcc aacgacgcca
    22681 ccatcaaggg cggcacctac tacccgatga cggtgaagaa gcacctgcgc gcgcaggaga
    22741 tcgcggcgca gaaccggctg ccctgcatct accttgtcga ttcgggcggc gggttcctgc
    22801 ccgagcagga caacgtgttt cccgaccgcg agcatttcgg gcgcatcttc ttcaacatcg
    22861 ccaacatgtc ggcggcgggc atcccgcaga tcgcggcggt gatgggctcg tgcaccgcgg
    22921 gaggggcata cgtcccggcc atgtcggacg agtcgatcat cgtcaagggc cagggcacga
    22981 tcttcctcgg cggaccgccg ctggtgaagg ccgcgaccgg cgaggtcgtg acgccggagg
    23041 agctcggtgg cgccgacgtg cacaccaagg tgtcgggggt gtccgaccac tacgcgcaga
    23101 acgacgcgca cgcgctcgcc atctgccgcc gcatcgtcgc ggggctgaac acgcgcaagc
    23161 aggtgtcgct cgacgtgcgc gcgccgcgcg agccgctcta ccccgccgag gagatctacg
    23221 gcgtgatccc ggccgaggtg cgaagaccct acgaggtgcg tgaggtgatc gcgcgcatcg
    23281 tcgacgcctc ggagctcgac gagttcaagc agaactacgg caccaccctc gttaccggct
    23341 tcgcgcacct gcacggctac ccggtggcga tcctcgccaa caacggcatc ctttattccg
    23401 agagctcgct caaggcggca cacttcatcg agcttgccgc gcagcgcggc attccgctcc
    23461 ttttcctgca gaacatcgcc ggctacatgg tgggcaagaa gtacgaggcc ggcggcatcg
    23521 ccaaggacgg cgccaagatg gtgaccgcgg tggccaccgc gcaggtgcca aaattcaccc
    23581 tgatcctcgg cggctcgttc ggtgcgggca actacggcat gtgcgggcgc gcctacgcgc
    23641 cgcgctttct ctggatgtgg ccgaacgccc gcatctccat catgggcggc gagcaggcgg
    23701 cgagcgtgct cgccaccgtg aggggcgagt tcaagtcgaa ggccgaggag gacgcgttca
    23761 agaacccgat tcgcgagcag tacgagcgcc agggccaccc ctactactcg agcgcgcgcc
    23821 tgtgggacga cggcgtggtg gaccccgcgg acacgcggcg cctgctcgcg ctcgcccttt
    23881 cggcctcgct caacgcgccg atccggccga cgaagttcgg cgtcttcagg atgtagccgt
    23941 gtcggagcac gaggcccacc gcaagcgctt catctccggc tcggtaagag ccgaggtcac
    24001 ggtcgaactg gagatcgcct gagatgctga aggtcgaaac cagggacggc gtcgcgcgcc
    24061 tgatgctcga tcgcccggag atccgcaacg cgttcgacga cgcgctcatt gccgcggtga
    24121 ccagggcgct gcgcgagctc gatgccgaca aggaggtgcg cgccgtggtg ctcggcggca
    24181 acggccccgc gttctgcgcc ggcggcgacc tcaattggat gaagcgcatg tcgggctaca
    24241 cctacgagca gaacctcgct gacgcgaaag ggctcgccgg catgctgcag acgctcgatc
    24301 gcatgaagaa gccgaccctt gcgcgcgtgc acgggcccgc ctacgcaggc ggcatcggcc
    24361 ttgttgccgc ctgcgacatc gcggtcggcg tgccggaagc gaagttctgc ctgtccgaag
    24421 ccaagatcgg gttgtcgcct gccaccatta gtccttacgt ggtgcgggcg atgggggaaa
    24481 ggatggcgcg gcggtatttc ttgtcggctg aagttttcga cggaatcgag gcgcatcgca
    24541 taggtttgtt gtcgcatctc tcggcgtcgg aaaagctcga cgccgagatc gatgaaattc
    24601 tgaagaacct cttggcgggc gggccggaat cgctggcgaa gatcaaggac ctgatccgct
    24661 tcgtcgcctc gcgccaggtg gacgacgcca tgatcgccga cacggcaaag cgcatcgccg
    24721 agatccgcgc cacgcccgag ggcaaggaag gcgtggcgtc gttcctcgag aagcgcaggc
    24781 ccgcctggaa gcgttgatgt tccgaaagat cctcatcgcc aaccgcggcg aaatcgcctg
    24841 ccgcgtcgcg gcgacagcgc ggcgcctggg gatcggcgtt gccgcggtgt actccgaagc
    24901 cgacgccggc gcgcgccacg tgaagctcgc cgacgaggcc tatctcctcg gcccggcgcc
    24961 ggcggcggag agctacctgc gcggcgagcg ggtgatcgag gcggcaa
//
LOCUS       DBNHKE010000175        24374 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_982510, whole genome shotgun sequence.
ACCESSION   DBNHKE010000175 DBNHKE010000000
VERSION     DBNHKE010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 24374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_982510"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="AC16O9_12025"
     CDS             <1..550
                     /locus_tag="AC16O9_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517380.1"
                     /translation="AAAARLAMLYVQAFDLGAGNALPYRRLDYGRLRAWLRAILDTDP
                     RSAYPLFAAARVYAEVDDPARARVMLEFIHEAFLQDPNRRWPALAHAALLAKHRLKDL
                     PLARRYAAELERRTTDPGVPLWAKQMEIFILEDMNELDAAKIMLGGLLASGRLQDPAE
                     RRFLKERLDALERRLASKPRRD"
     gene            674..1360
                     /locus_tag="AC16O9_12030"
     CDS             674..1360
                     /locus_tag="AC16O9_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR6517381.1"
                     /translation="MKTNQKGFTLVEIAIVLVIIGLLLGGILKGQEMITQAKIKNIIS
                     DFSGISAAFHGYQDRYRALPGDDNGAAARWAAAPAATSGNGNGVVAGTYNNAGAACAA
                     AVEACSWWDHLRRSGFVAGSGVNQPLNAVSGVVGVQTGDGAAGAVLGGVGGVGGFFGL
                     VMCSANHPDKVAIASDTQMDDGVRTTGSVRGQLQTTNNPNIVQNALTAPDANSYVESG
                     TNTYTLCRLL"
     gene            complement(1414..3396)
                     /locus_tag="AC16O9_12035"
     CDS             complement(1414..3396)
                     /locus_tag="AC16O9_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6517382.1"
                     /translation="MDATGLALLPVLVLLAGGVLAVVACRSLGLPPIIGYLVAGVVLG
                     PHAAGLVADTEAVRHLAEFGVVFLMFSIGLEFSLPKLHAMGRAVFGLGLSQVAVSIAL
                     GVAAALAVGGSWQAGIALGGAVAMSSTAMVSKLLAERLELDTAHGRDVIGVLLFQDLA
                     VVPLLILVPALGGPVEGIGADIAEALAKAAAVLAVLLLAGPRMMRAWFGVIARRRSTE
                     LFVLNVLLITLLLAFITGFAGLSLALGAFLAGMLISETEYRYQVEEDIKPFRDVLLGL
                     FFVTVGMMLDLRLVAAQGWLVLLLVAVLLLAKGALIGLLARAFGAAPGTALRTALALA
                     QGSEFGFVLLAQARGAELAPEPVLQALIAAMILSMLATPFLVAASDRIVLRFTASEWM
                     TRSLELHRVAVSSLQTERHVIILGYGRNGQRLARLLEAEGVRYVALDLDPERVREAAL
                     AGDTVVYADCSRREALVAAGVARAAAVVVTFSDVAAAARVLANVHALNAAVPVIVRAR
                     DEADIARLTAAGASEVVPEAFESGLMLASHTLVWLGVPLARVMRRVSQMREQQYGLLR
                     GLYHGTSDAPEGGEALQPRLHAVTLHAHAAALARPLAALGIEELGAQVRAVRRPGAAK
                     KLAPEEAGALRAGDVVVLLGTPQALAAAELRLLEGQ"
     gene            3443..4465
                     /locus_tag="AC16O9_12040"
     CDS             3443..4465
                     /locus_tag="AC16O9_12040"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR6517383.1"
                     /translation="MISAVRSERQRATRSGDSALELARLVLAVEADAVRALIERLDGR
                     FLAAVHLIQDCVDSKRGRVIVSGIGKSGHIARKIASTMASTGTPAYFVHPAEASHGDL
                     GMIRAEDVFIGISNSGESAELLRIVPAIKREGAKLVAITGSAESSLAREADVHLDAGV
                     AKEACPLNLSPTASTTAALAMGDALAIALLDARGFSAADFARSHPGGSLHREALKHVA
                     DVMHRGADVPRVRATATLKQALDEMTRGGLGMTAVVDAARRVQGIFTDGDLRRALKKV
                     RSLKAARIAQVMTRGPRTIAPEALATEAVALMERHRISQLLVVDAAGELVGALNMNDL
                     LRAKVV"
     gene            4470..5006
                     /locus_tag="AC16O9_12045"
     CDS             4470..5006
                     /locus_tag="AC16O9_12045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR6517384.1"
                     /translation="MAQASADALERAARLRLMVFDVDGVLTDGRLWYGPGGEALKAFH
                     VRDGHGLKLLGEYGVAAALLTGRRSAALVARAAELGLRHVLQGVEDKRPAFERLLAQL
                     GIEPAAAGYMGDDLPDLPVLGHCGFACAPPGAHALALRRAHYVPDAPAGAGAAREVCE
                     YVLGAQGRLEDALARYLA"
     gene            5003..5575
                     /gene="lptC"
                     /locus_tag="AC16O9_12050"
     CDS             5003..5575
                     /gene="lptC"
                     /locus_tag="AC16O9_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR6517385.1"
                     /translation="MRLTGTRLFPLALMLSLALLSFWLERTAREAPAPATQPRHDPDY
                     SVEQFTITDFSRAGVPESTLSAAKMVHYPDDDTTALVAPRLVQAKPAQPRLILSADRG
                     MLSRDGAEVFLHDNVLLLREALAESAEGRMQSSFLHVIRDRSLVRTDREVRISERGRT
                     LVGLGMEYDNQARRLELQAQVRGVFEGEPE"
     gene            5575..6147
                     /gene="lptA"
                     /locus_tag="AC16O9_12055"
     CDS             5575..6147
                     /gene="lptA"
                     /locus_tag="AC16O9_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR6517386.1"
                     /translation="MRRRLAIALAAAIAAVSAFSAKAEKADREKPTQVEANRMTSDEA
                     RRISIFEGNVVLTKGTLQLQAERIVVRQDADGFQTASASGNPVRFRQKSDPRGGAEGV
                     WIEGQAGRIEIDEKNERIELHQNAQVTRDKDEVRGSYILYDQRSEFFSVSSAKDAPGS
                     RVRAVIQPKPAAADAPAPAKPEAAKPEAKK"
     gene            6144..6866
                     /gene="lptB"
                     /locus_tag="AC16O9_12060"
     CDS             6144..6866
                     /gene="lptB"
                     /locus_tag="AC16O9_12060"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR6517387.1"
                     /translation="MSLLRCERLRKRYKSRAVVQDVSLEVQSGEVVGLLGPNGAGKTT
                     CFYMIVGLIAADAGRIHLDGRELTHLPIHARARLGISYLPQENSVFRKLTVEENVQAV
                     LELQALARADIEARLQELLKDLNIAHLRKHAALSLSGGERRRLEIARALATKPAFILL
                     DEPFAGVDPIAVLDIQRIIRFLKERAIGVLITDHNVRETLGICDRAYIINEGAVLASG
                     HPEEIVYNDSVRRVYLGEHFRM"
     gene            6887..8302
                     /locus_tag="AC16O9_12065"
     CDS             6887..8302
                     /locus_tag="AC16O9_12065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR6517388.1"
                     /translation="MKPTLQFRLSQHLTLTPQLQQSIRLLQLSTIELNQEIERLLMEN
                     PILEREDVEGDAQAAFTPPRDTLQSASEAATQAADETTERREDWLAELAAPAWRGDDD
                     EDGDRTLAAPDTPTLRDHLNQQLTLTGIGERDRALVALLIDVLDDDGYLTQPLEEIAA
                     LFAAEAQVDLEELSIALKHLQNLEPAGVGARSPAECLLLQLRAHAGHPACALASEIAA
                     RHLALLAGRDYTRLRALTGAQEADLRAAQKLIQSLNPHPGAAFARVQTRYMIPDVIVR
                     KTRNVWRASLNPEAMPRLRINRLYADLAGSARNGSSALASQLQEARWLIKNVQQRFDT
                     ILRVSQAIVDRQRHFLEHGEVAMRPMVLREIAETLGLHESTISRVTTQKFMATPRGTF
                     ELKYFFSSQLATDAGGATSSTAIRALIKQLVGAEDAHAPLSDARIAQLLGQQGIVVAR
                     RTIAKYRESLQIPPVNQRKSL"
     gene            8313..8633
                     /gene="hpf"
                     /locus_tag="AC16O9_12070"
     CDS             8313..8633
                     /gene="hpf"
                     /locus_tag="AC16O9_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6517389.1"
                     /translation="MNLNLSGHHLEITPSLRSYVEEKLARVTRHFDHVIDAHVVLSVD
                     KLRQKAEVTLHMRGKDIHCASEEQDLYAAIDLVTDKLDRQVLKYKSKRSAKPHEARKR
                     ADNV"
     gene            8716..9201
                     /gene="ptsN"
                     /locus_tag="AC16O9_12075"
     CDS             8716..9201
                     /gene="ptsN"
                     /locus_tag="AC16O9_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HIR6517390.1"
                     /translation="MSLVAKLLPPANVLLDLQVSSKKRLFEQVGLMFENHNGIARSLV
                     FDSLFARERLGSTGLGQGVAIPHGRIKGLKDALGAFVRLAQPVPFDAPDTKPVTLLFV
                     LLVPEQATEKHLQILSELAQMFSDRALRDAMAGAADAAALHQLIAAWQPDAAGQRQAV
                     A"
     gene            9170..10078
                     /gene="hprK"
                     /locus_tag="AC16O9_12080"
     CDS             9170..10078
                     /gene="hprK"
                     /locus_tag="AC16O9_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR6517391.1"
                     /translation="MQQVNVKRLHEVNREALALAWVAGRDADPAVRREPGAAAALIGH
                     LNLTHPNRIQVAGADEAGALERLAEALFAAAPAAVILADGVAPPAALLERAERARTPL
                     FTTPLAAARVIEKLARYLAKALAESTERHGVFMDVLGLGVLITGDSGVGKSELGLELI
                     SRGHGLVADDMVEISRLAADTLEGRCPPVLKDFIEVRGLGLLDVRTIFGETAVRPKMK
                     LRLIAHLDRPAAVAGRDVAERLPLAEQSEDILGVTVRKVVIPVAAGRNLAVLLEAAVR
                     NYILQLRGIHSGAEFMARQKRAMDGD"
     gene            10089..10928
                     /gene="rapZ"
                     /locus_tag="AC16O9_12085"
     CDS             10089..10928
                     /gene="rapZ"
                     /locus_tag="AC16O9_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR6517392.1"
                     /translation="MQIILVSGLSGAGKSIALDVLEDAGYYCVDNLPATLLMDVVGFL
                     ADAGHERAALSVDARSAALAALPKSVARLRADGADCRVLFLEANAPTLVRRFSETRRR
                     HPLTVQGLTLAEAIERERTLMGEIAELGHRIDTSELQPKVLRNWIRDLLGLGAGRLVV
                     LFESFAFRDGLPLDADWVIDARMLPNPHYDPQLRPLTGRDAAVIAYLDGQEAVQRFFA
                     DVRAFLGRWLPEVVKENRSYLTVAIGCTGGRHRSVYLAERLAAAFAAESRVLVRHRGL
                     ARE"
     gene            10925..11518
                     /locus_tag="AC16O9_12090"
     CDS             10925..11518
                     /locus_tag="AC16O9_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6517393.1"
                     /translation="MSDADLADLPLFALQTVLFPGGRLPLRIFEQRYMDMAKACLRDD
                     SPFGVCLIREGGEVGAPAVPEEVGCLARIVAWDMPQLGVLQVQARGEQRFRIRKRRVQ
                     ADGLVRASIELLAAELDAKLEPDTDLCARVLRKAVESGGEALVEPPLRYASAAWVSAR
                     LAELLPLSLDAKQRLLEMNDARDRLEILRRLISSAGQ"
     gene            complement(11495..12472)
                     /gene="mutY"
                     /locus_tag="AC16O9_12095"
     CDS             complement(11495..12472)
                     /gene="mutY"
                     /locus_tag="AC16O9_12095"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR6517394.1"
                     /translation="MRQFAPRLIAWQRRHGRHDLPWQGTRDPYRVWLAEVMLQQTQVE
                     TVIPYYRRFLARFPGVAVLASATEDEVLRRWSGLGYYARGRNLHAAARAVVARGGFPR
                     DAAGLQALPGVGRSTAAAIAVFAFGRRAAILDGNVKRVLARRFGVQGEKAQWDLAEEL
                     LPRKGVRAYTQALMDLGALVCTRTRPACERCPVATGCVARREGRIAELPAARARKPLP
                     LKRAHWFVYVRGAEVLLERRPGAGLWGGLWTFPEARLPAAPRGRKLALLEHGFTHFRL
                     RAQPWLCALRGATPAGANRLWIDMAEATGAAVPAPVRRLLRSLLAGRAD"
     gene            12508..13491
                     /locus_tag="AC16O9_12100"
     CDS             12508..13491
                     /locus_tag="AC16O9_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6517395.1"
                     /translation="MLAFVLRRLGQAVLVMLAVGLIAFALFRFVGDPVLFMLGQDATP
                     EDRERITRLLGLDQPFYVQYAKFVGRAVQGEFGLSLRQVQPVSKLLVERLPATLELSF
                     VAAAFALVAGIAMGVYTALRRHTWLSQLLLAVSLLGVSLPTFLIGILLILVFAVLLGW
                     LPSFGRGETVMIGWWSTGLLTWSGIKALILPAVTLGLFQMTLIQRLVRAEMLEVLRTD
                     YIKFARARGLTDRAVHFGHALKNTLVPVITITGLQLGAIIAFAIITETVFQWPGMGLL
                     FIQAVGFADVPVMAAYLCLIALFFVVINLAVDLLYYAVDPRLRVERSAMAQ"
     gene            13499..14683
                     /locus_tag="AC16O9_12105"
     CDS             13499..14683
                     /locus_tag="AC16O9_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HIR6517396.1"
                     /translation="MKNDPLEMLRLYHADLVHIRRDIHAHPELGFEETRTSQLVAERL
                     ADWGIEAHRGLAKTGVVGVVKGRKDGGRAIGLRADMDCLPLHETGDAPHKSKFAGRMH
                     ACGHDGHTTMLLGAARYLAQTRNFDGTVHLIFQPAEEGGGGGRVMVEEGLFEKFPCEA
                     VYALHNWPGLPSGKIAVRPGPMLAATDEIKLKVRGKGGHAALPHLATDPVVIAAHIIT
                     ALQTIASRNANPVDAVVVSLCSMQTSQLGAFNIIPTHVELVGTVRTFTPEMRDLAERR
                     IREIADAVALGLGGSAEINYRRGYPATVNTEKEAHFAAQVGEKVFGKANVITDHEPTM
                     GGEDFAYMLQAKPGAYVFLGQGGAQGGCFLHNPTYDFNDEVIPLGAGYLAALAEAALP
                     LK"
     gene            14701..16266
                     /locus_tag="AC16O9_12110"
     CDS             14701..16266
                     /locus_tag="AC16O9_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6517397.1"
                     /translation="MKLKHLIAAALVAVVLPAHAATLRWAAQNDILTLDPHSQNHATT
                     SSLLQHAYEGLTRYAKDYSIEPALATGWQQLSPTHLRFTLRKGVKFHDGSPFTAEDVV
                     FSYGRILQPQGTMQIYVGGVKEVKKVDDYTVDFMLAGPNPVLLRNIADFRIMSKTWAM
                     KHKSENVQNYVAKEESYASRNTNGTGPYIIKGWEPDKRIVMTANPEWWGKREGNVTDV
                     VYTPIKADATRIAALLSGDVDMVTDLPTQDVTRLRSEAKLKVLDGHEVRTIFIGMDQH
                     SDELKYSSVKGKNPFKDVRVRKALNLAVDREAIRRVTMRGLSIPAAIMVAPGVHGHAK
                     DIDQALALDVEGAKKLLADAGYGSGFEFGLDCPNNRYVNDEEICQALVGMWARIGLKA
                     KLNAQPMATFIQKIQKFDHDAYMLGWGVPNFDANYTLQSLVRTKTTGADGSFNLGRIS
                     DAKIDALVDAIKTELDTAKRDAMLREALVLTRDNHYYVPLHHQLRPWAMKKNVTAVHN
                     AVDRHESRYTRVE"
     gene            16272..17183
                     /locus_tag="AC16O9_12115"
     CDS             16272..17183
                     /locus_tag="AC16O9_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6517398.1"
                     /translation="MSWLRRAWDHDLAWSFRHSPVALAAAVLTALCVGGALLAPWLAP
                     HDPFNSATLNLSEGFTPPAWSDKGSAKYLLGTDDQGRDVLSTIMYGARISLGVGLASV
                     LFSALLGVSLGLVSGYLGGRLDAFIMRIADIQLSFPAILVALLIDGIARIALPRELHD
                     ALAVYVLVLAIGLSGWVQYARTVRGSTLVEKGKEYVQAARVIGRHPLAVMLHHVLPNV
                     MGPVLVIATIHVATAIITEATLSFLGVGVPPTQPSLGTLVRIGNDFLLSGEWWIALFP
                     GAALVILVLAVNLLGDWLRDALNPKLR"
     gene            17180..18148
                     /locus_tag="AC16O9_12120"
     CDS             17180..18148
                     /locus_tag="AC16O9_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517399.1"
                     /translation="MSAPLLEVRDLRVEFPTRRGTLVAVDGVSFAIAPGEVLGVVGES
                     GAGKSLTGTAIIGLLEPPGRIAAGEIRLEGRRIDELPPGEMRKLRGRRIGAIFQDPLT
                     SLNPLYTVGDQLVETILTHLDMSRDEARRRALHLLAEVGIPAPDARIDQYPHQFSGGM
                     RQRVVIALALAAEPRLIIADEPTTALDVSIQAQIITLLKRLARDHRTAVMLITHDMGV
                     IAETADRVAVMYAGRIAEIGPVREVLQAPRHPYTAGLMGSIPKLGTSRRHARLVQIDG
                     AMPRLTAIPAGCAYHPRCPSRFARCDAERPELLPVGNARASCWLYG"
     gene            18141..19127
                     /locus_tag="AC16O9_12125"
     CDS             18141..19127
                     /locus_tag="AC16O9_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517400.1"
                     /translation="MDSRPLLRLEGASRDFDVSRPWLNRLLEGAPRQLLRAVDDVSFE
                     VARGETLALVGESGCGKSTVARLIVGLYPLTRGRIEFDGADLSTAGAHRQRRRMQMIF
                     QDPYASLNPRWRVRDIVAEPIRVLGLTASKDAERARVAELLRQVGLAPEDGEKYPHEF
                     SGGQRQRISIARALSGNPEFLVCDEPTSALDVSVQAQILNLMTELQARLGLTYLFISH
                     NLAVVSHVADRVGVMYLGRVVEIAPAAQLFAAPRHPYTRMLMDAIPDLEMSGKARTPV
                     AGEVPNPLAPPAGCAFHPRCPHADARCRSERPRLRPAAGGAVACHAVEEGRL"
     gene            complement(19124..20962)
                     /locus_tag="AC16O9_12130"
     CDS             complement(19124..20962)
                     /locus_tag="AC16O9_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6517401.1"
                     /translation="MKWLKYVLYSLLGVIVLALVGVAVIVATFDPNAYKGEIEKRVKE
                     ATGRTLKFHGDIALALWPKIGAKVGKVTLSRRASEHDFAAIDGAQVAVALLPLLSGQV
                     QVDEVTLTGLRASVIRAKNGKFDFEDLLGAGGGAGKAPAARGAAPRKVAFDVAGIRLR
                     DARFAYLDEGSGQRLELDDVTLTTGRIADDVPGTLALGARVRGKKPLIDLKMDLAGTY
                     RLALERREYSLTGMNLGVSGTADEFTRIALTVTGDATARLAKEALDADLVAKFDDTTV
                     KMKLGMAGFDAPSYRVDAAVDRIDLDRYLVAQKDAKPAPSAGPKVAVDVPVDLSALEG
                     LRASGKLSIGWMQLMGLKVSDLKAELRAANGRADIAPHSAKLYEGGLEGAMTLIARGN
                     RIAVKETLRGVAIGPLVKDLMARDAVEGRGDVAVDVTASGPTVNALKKSLAGTARVEL
                     KDGAVKGINLAEVLRKTRAAFGSKSAQEQPSDTSKRTDFSAMSASFVIKDGVARNDDL
                     DVRAPALRVGGAGDIDIGNSRLDYLAKASVVASAEGQGGAGLEHLKGLTIPVKLTGPF
                     DAPKYEIDYRALAGDAAKAKVKEKAKKAEEKIEKKLEDKLRGLIRR"
     gene            complement(20959..21861)
                     /locus_tag="AC16O9_12135"
     CDS             complement(20959..21861)
                     /locus_tag="AC16O9_12135"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HIR6517402.1"
                     /translation="MPVTFDGKLVVAISSRALFDFEEENRVFDRDGEPAYVTLQLGRL
                     DVPAKRGVAFPLVQKLLAFNTPEAQRVEVVILSKNDPVSGLRVFRSAKREGLSVERGV
                     FTRGRDPYRYLDALRANLFLSANERDVMAALDARIPAARVVPESAQAASRHADEVRIA
                     FDGDAVLFSDEAERVYQADGLEAFTRHETDRVNTPLPPGPFKPLLEALHRLQTAKAPM
                     RLRTALVTARSAPAHERAVKTLMHWKISVDEALFLGGLDKGEFLGAFEPDFYFDDQRG
                     HVESAGRHVATGHVPFGIAGRKPA"
     gene            complement(21868..21941)
                     /locus_tag="AC16O9_12140"
     tRNA            complement(21868..21941)
                     /locus_tag="AC16O9_12140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21907..21909),aa:Gly,seq:ccc)
     gene            21974..22174
                     /gene="thiS"
                     /locus_tag="AC16O9_12145"
     CDS             21974..22174
                     /gene="thiS"
                     /locus_tag="AC16O9_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR6517403.1"
                     /translation="MQVTVNGAARRFERPLAVNELVAELALSGKKIAVERNGEIVPKS
                     AHGSTLVADGDRLEIVVAVGGG"
     gene            22171..22974
                     /locus_tag="AC16O9_12150"
     CDS             22171..22974
                     /locus_tag="AC16O9_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR6517404.1"
                     /translation="MSDRLLVAGKAYGSRLLIGTGKYKDFAQTRAAVEASGAEIVTVA
                     IRRTNIGQNANEPSLLEALPPSKFTYLPNTAGCYSAEDAVRTLRLARELLDGHDLVKL
                     EVLGDPHTLYPNMPETLKAAGELVRDGFKVMVYCSDDPIQAKILEDAGCVAIMPLASL
                     IGSGMGILNPWQVQLVRDAVKLPVIVDAGVGTASDAAIAMELGCDGVLMNTAVAAAKD
                     PVMMARAMKKAVEAGREAWLAGRMPKKLYTAAPSSPTEGTIAPSRSKAA"
     gene            22971..23603
                     /gene="trmB"
                     /locus_tag="AC16O9_12155"
     CDS             22971..23603
                     /gene="trmB"
                     /locus_tag="AC16O9_12155"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR6517405.1"
                     /translation="MKRGIRSYVLRQGRTTPAQQRALDELFTVYGIPFRQALLEPRGK
                     PLVLEIGSGMGETTAAIAAAQPGSDFIAVEVHGPGVGSLLRRIDEQGLKNLRVIRHDA
                     IEVLEHMIADASLAGIHLFFPDPWPKKRHHKRRLVQPAFATLAARKLAPGGYLHAATD
                     WPQYAEQMAEVLDAEPLLTRAAARERPATKFEQRGMKLGHEVRDLVYRRR"
     gene            complement(23605..23970)
                     /locus_tag="AC16O9_12160"
     CDS             complement(23605..23970)
                     /locus_tag="AC16O9_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR6517406.1"
                     /translation="MRLDKWLWAARWFKTRGLAQLAIASGRVKLHEEKTKPAHALKVG
                     DQLEIRVGESVWQVEVTALSDKRGPAAQARKLYAESEASRAERERRADLRRHSAEPAR
                     TIKGRPTKRDRRKLDELKS"
     gene            complement(23973..>24374)
                     /locus_tag="AC16O9_12165"
     CDS             complement(23973..>24374)
                     /locus_tag="AC16O9_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233494.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6517407.1"
                     /translation="PGRRCRHNLNYWEFGDYLGIGAGAHGKLSFPDHVARHERAKQPR
                     EYLAQAGSAVDKPIAPRELPFEFMLNALRLVQGVPKVRFEERTGLPLAAVARKLAAAE
                     EKGLLENDPARIRPTRRGRLFLNELLQMFLD"
BASE COUNT         3720 a         8804 c         8326 g         3524 t
ORIGIN      
        1 ggcggccgcc gcgcgcctcg ccatgctcta cgtgcaggca ttcgatctcg gcgccggcaa
       61 cgccctgccg taccggcgcc tggactacgg ccgcctgcgg gcctggctgc gggcgatcct
      121 cgataccgac ccgcgcagcg cctaccccct gttcgccgca gcccgggtgt acgccgaggt
      181 cgacgacccc gcgcgtgccc gggtgatgct cgagttcatc cacgaggcct tcctgcaaga
      241 ccccaaccgg cgctggcctg ccctcgccca cgcggcgctc ctcgccaagc atcgcctgaa
      301 agacctgccg ctcgcgcgcc gctacgcggc cgagctcgag cgccggacca ccgacccggg
      361 cgtgccgctg tgggcgaagc agatggagat cttcattctc gaggacatga acgagctcga
      421 cgccgcgaag atcatgctcg gcggcctgct cgccagcggc cgcctgcagg acccggccga
      481 gcggcgcttc ctgaaggagc gcctcgatgc cctggagcgc cgattggcgt cgaaaccgcg
      541 acgcgattga cggaattccg acaccttagg ccgtgactta taccctcacg gggtccgaaa
      601 tgccaagatt ttccaggcac ggaacttgcg tccgcattta tcgcctcgtc ctaacggcaa
      661 aaggagcaat gggatgaaaa cgaaccaaaa aggcttcacg ctcgtggaaa tcgcgatcgt
      721 gctggtgatc atcgggctgc tgctgggcgg catcctgaag ggccaggaaa tgatcacgca
      781 ggccaagatc aagaacatca tttcggactt ctccggcatc tcggcggcct tccacggcta
      841 ccaggaccgc taccgcgccc tgccggggga cgacaacggt gcggccgccc gatgggcggc
      901 ggcgccggcc gccacctccg gcaacggcaa cggcgtggtc gccgggacct ataacaacgc
      961 cggcgccgct tgcgcagccg cggtcgaagc ttgcagctgg tgggatcacc tgcgccgctc
     1021 cggtttcgtc gctggctcgg gtgtgaacca gccgcttaac gctgtgtccg gtgtcgtcgg
     1081 cgtgcaaacg ggcgatggcg ctgccggagc ggtcctgggc ggcgtcgggg gcgtcggcgg
     1141 gtttttcgga ctggtaatgt gctcggcgaa ccacccggac aaggtcgcga tcgccagcga
     1201 tacgcaaatg gacgacggcg tgcgcacgac gggctccgtg cgcggtcagc tgcagacgac
     1261 aaacaacccg aatatcgtcc agaacgcctt gacggcgccc gacgccaaca gctacgtgga
     1321 gagcggcacc aacacctaca cgctctgccg cctgctgtaa gcgcagctgt tcaatcgccc
     1381 gaagggccgg ccacgcgccg gcccttttct tttctactgc ccttccagca gccgcagctc
     1441 ggccgcggcg agcgcctgcg gcgtgccgag cagcaccacc acgtcgcccg cgcgcagggc
     1501 gccggcctct tccggcgcca gcttcttcgc cgccccgggg cggcgtaccg cgcgcacctg
     1561 cgccccgagc tcctcgatgc cgagtgcggc cagcgggcgc gcgagcgcgg cggcgtgcgc
     1621 gtgcagcgtc accgcatgca ggcgcggctg cagcgcctcg ccgccctccg gggcgtcgct
     1681 cgtgccgtga tacagcccgc gcagcagccc gtactgctgc tcgcgcatct ggctgacgcg
     1741 gcgcatgacg cgcgcgagcg gcacgcccaa ccagaccagc gtgtgcgagg cgagcatcag
     1801 gcccgactcg aaggcctcgg gcaccacttc agaggcgccc gcggcggtga ggcgcgcgat
     1861 atctgcctcg tcgcgcgcgc gcacgatcac tggcaccgcc gcgttcagcg cgtgcacgtt
     1921 cgccagcacc cgcgccgctg ccgccacgtc ggagaacgtg acgaccaccg ccgcggcgcg
     1981 cgccacgccg gccgcgacca gcgcctcgcg ccgcgagcaa tcggcgtaca ccaccgtgtc
     2041 gccggcgagc gccgcctcgc gcacgcgctc gggatcgagg tcgagcgcga cgtagcgcac
     2101 cccttcggcc tcgagcaggc gcgccaggcg ctggccgttc ctgccatagc cgaggatgat
     2161 gacgtggcgc tcggtctgga gcgagctcac cgccacccgg tgcagctcca gcgagcgcgt
     2221 catccactca gacgcggtga aacgcagcac gatgcggtcg ctcgcggcga ccaggaacgg
     2281 cgtcgccagc atggacagga tcatcgccgc gatcagcgcc tgcagcaccg gctcgggcgc
     2341 aagctcggcg ccgcgcgcct gcgccagcag cacgaagccg aactcgctgc cctgcgcgag
     2401 cgccagcgcc gtgcgcagcg ccgtgccggg agcggcgccg aaggcgcgcg cgagcagccc
     2461 gatcagcgcg cccttggcca ggagcagcac cgcgaccagc agcagcacca gccagccctg
     2521 cgccgccacc aggcgcaggt cgagcatcat gccgacagtg acgaagaaca ggccgagcag
     2581 cacgtcgcga aacggcttga tgtcctcctc cacctggtag cggtattcgg tttccgagat
     2641 cagcatgccg gcgaggaagg cgccgagcgc gagcgacagc ccggcgaagc ccgtgatgaa
     2701 tgcgagcagc agcgtgatga gcaggacgtt cagcacgaac agctccgtgg agcggcgccg
     2761 cgcgatgacg ccgaaccagg cgcgcatcat gcgcggcccg gcgaggagca gcaccgcgag
     2821 cactgccgcc gccttggcga gcgcctccgc gatgtcggca ccgatgcctt ccaccgggcc
     2881 gccgagcgcc ggcaccagga tcagcagcgg caccaccgcg agatcctgga acaacagcac
     2941 gccgatcacg tcgcggccgt gcgccgtgtc gagctccagg cgctcggcga gcagcttcga
     3001 gaccatcgcc gtcgaggaca tcgcgaccgc gccgccgagc gcgatgccgg cctgccagct
     3061 gccgccgacg gcgagcgcgg cggccacgcc gagcgcgatg ctgacggcga cctgcgacag
     3121 ccccagcccg aacaccgcgc ggcccatggc gtgcaatttc ggcaggctga actcgagccc
     3181 gatcgagaac atcaggaaca ccacgccgaa ctcggcgagg tggcgcacgg cctcggtgtc
     3241 ggcgaccagg cccgccgcat gcggcccgag caccacgccg gccaccaggt agccgatgat
     3301 gggcggcagc cccaggctgc ggcacgccac cacggcgagc accccccccg cgagcagcac
     3361 gaggaccggg agcagcgcca ggccggtggc gtccatttcc gctggaaggg tcggcgaagt
     3421 gggagagtta tacttcgcga ctatgataag cgcagtgcgc tccgagcggc agcgcgcgac
     3481 gcgttcgggc gacagtgcgc tggagctcgc tcgcctcgtg ctggcggtgg aagccgacgc
     3541 ggtgcgcgcg ctgatagagc gtctcgacgg gcgcttcctc gccgcggtgc acttgatcca
     3601 ggactgcgtc gacagcaagc gcggccgcgt catcgtcagc ggcatcggca agtccggcca
     3661 cattgcgcgc aagatcgcct cgaccatggc gagcaccggc acgccggcct acttcgtgca
     3721 tccggccgaa gcgagccacg gcgatctcgg catgatccgc gccgaggacg tgttcatcgg
     3781 catttcgaat tcgggcgaga gcgccgagct gctgcgcatc gtgccggcga tcaagcgcga
     3841 aggcgccaag ctggtcgcga tcaccggaag cgcggagtcg tcgctcgcgc gcgaggccga
     3901 cgtgcatctg gatgcggggg tggccaagga ggcctgcccg ctcaacctgt cgccgaccgc
     3961 cagcaccacg gccgcgctcg ccatgggtga tgcgcttgcc atcgcgctgc tcgacgcgcg
     4021 cggcttttcc gccgccgatt tcgcgcgctc ccacccgggc ggatcgctgc atcgcgaggc
     4081 gctgaagcac gtcgccgacg tcatgcaccg cggcgccgac gtgcccaggg tgcgggcaac
     4141 cgcgacgctc aagcaggccc tcgacgagat gacgcgcggc ggcctcggca tgaccgcggt
     4201 cgtggatgcg gcgcggcgcg tgcagggcat cttcaccgac ggcgacctgc gccgcgccct
     4261 gaagaaggtc cgaagcctga aggcggcgcg catcgcgcag gtgatgaccc gcggcccgcg
     4321 caccatcgcc ccggaggcgc tcgcgaccga ggcggtggcg ctcatggaac gtcaccgcat
     4381 cagccagctc ctggtggtgg atgcggctgg agaactggtc ggcgcgctca acatgaacga
     4441 tctgttgcgc gccaaggtgg tctgaagcga tggcgcaggc atccgccgac gcgctcgagc
     4501 gcgccgcgcg cctccggctc atggtgttcg acgtggacgg cgtgctcacc gacgggcgcc
     4561 tgtggtatgg cccgggcggc gaggcgctga aggcgtttca cgtgcgcgac gggcatgggc
     4621 tgaagctgct cggcgagtac ggcgtcgccg ccgcgctgct caccggccgg cgctccgcgg
     4681 cgctcgttgc gcgcgccgcg gagctcggcc tgcgccatgt cctgcagggc gtcgaggaca
     4741 agcgcccggc gttcgagcgg ctgctggcgc agctcggcat cgagccggcc gccgccggct
     4801 acatgggcga cgacctgccg gacctgccgg tgctcgggca ctgcggcttt gcctgcgcgc
     4861 cgccgggcgc gcacgcgctc gcgctgcgcc gcgcacacta cgttcccgac gcgccggccg
     4921 gcgccggcgc cgcgcgcgag gtgtgcgagt acgtgctcgg cgcccagggc cggctcgagg
     4981 acgcactcgc gaggtacctg gcatgaggct caccggcacg cgcctgttcc cgcttgcgct
     5041 gatgctctcg ctcgcgctgc tcagcttctg gctcgagcgc accgcgcgcg aggcgccggc
     5101 gcccgccacg cagccgcgcc acgaccccga ctacagcgtc gagcaattca ccatcaccga
     5161 cttcagccgc gccggggtgc ccgagtcgac gctgtcggcg gcgaagatgg tgcactaccc
     5221 cgacgacgac accacggcgc tggtggcgcc gcgcctggtg caggcgaagc ccgcacagcc
     5281 gcgcctcatc ctcagcgccg accgcggcat gctgtcgcgc gacggcgcgg aggtgtttct
     5341 ccacgacaac gtgctcctgc tgcgcgaagc gctcgccgag tccgccgagg gccgcatgca
     5401 gtcgagcttc ctgcacgtga tccgcgaccg ctcgctggtg cgcaccgacc gcgaagtgcg
     5461 catcagcgag cgcgggcgca ccctggtcgg cctgggcatg gagtacgaca accaggcgcg
     5521 gcggctggaa ctgcaggcgc aggtgcgggg cgtgttcgag ggggagccag agtagtgcgc
     5581 cggcggctcg ccatcgctct tgccgcagcg attgctgcgg tttcggcttt ttccgccaag
     5641 gcggaaaaag ccgatcggga aaagcccacg caggtcgagg ccaaccgcat gacctccgac
     5701 gaggcgcggc gcatcagcat cttcgagggc aacgtggtgc tcaccaaggg cacgctgcag
     5761 ctgcaggcgg agcgcatcgt cgtgcgccag gacgccgatg gcttccagac cgccagtgcg
     5821 agcggcaacc cggtgcgctt tcgccagaag agcgacccgc gcggcggcgc ggaaggcgtg
     5881 tggatcgagg gccaggccgg gcgcatcgag atcgacgaga agaacgagcg catcgagctg
     5941 caccagaatg cgcaggtcac gcgcgacaag gacgaggtgc gcggcagcta catcctctac
     6001 gaccagcgct cggagttctt ctcggtctcc agtgccaagg atgcccccgg ctcgcgcgtg
     6061 cgcgcggtga tccagccaaa gccggccgcg gccgacgcgc cggcccccgc caagcccgag
     6121 gcggccaagc ccgaggcaaa gaagtgagcc tgctgcgctg cgagcggctg cgcaagcgat
     6181 acaagtcgcg cgccgtggtg caggacgtct cgctcgaagt gcagagcggc gaggtggtcg
     6241 ggctgctcgg cccgaacggc gcgggcaaga ccacctgctt ctacatgatc gtcggcctga
     6301 tcgcggccga cgccgggcgc atccatctcg acggtcgcga gctcacgcac ctgccgatcc
     6361 acgcccgcgc gcgcctgggc atctcctacc tgccgcagga gaattcggtg ttccgcaagc
     6421 tcaccgtcga agagaacgtg caggcggtgc tcgagctgca ggcgctcgcg cgcgccgaca
     6481 tcgaggcgcg gctgcaggag ctgctcaagg acctcaacat cgcgcacctg cgcaagcacg
     6541 cggcgctgtc gctctcgggc ggcgagcgcc ggcgcctcga gatcgcgcgg gcgctcgcga
     6601 ccaagccggc gttcatcctg ctcgacgagc ccttcgccgg ggtcgatccg atcgccgtcc
     6661 tcgatatcca gcgcatcatc cgctttctca aggagcgcgc catcggcgtg ctcattacag
     6721 atcataacgt tcgcgagacg ctgggcatct gcgaccgcgc ctacatcatc aacgaaggcg
     6781 cggtactcgc ctcgggccac ccggaggaga tcgtctataa tgacagcgtg cgccgggtct
     6841 atctcggcga gcatttccgc atgtagccac cgcagttccc gccgccatga agcctactct
     6901 gcagttccgg ctctcgcagc acctgacgct gacgccgcag ctgcagcagt ccatccgcct
     6961 gctgcagctc tccaccatcg agctgaacca ggagatcgag cgcctgctga tggaaaaccc
     7021 gatcctcgag cgcgaggacg tggaaggcga cgcgcaggcc gcgttcacgc cgccgcgcga
     7081 caccctgcaa agcgcgtccg aggccgccac gcaggccgcg gatgaaacca ccgagcgccg
     7141 cgaggactgg ctggccgagc tcgcggcgcc cgcgtggcgc ggcgacgacg acgaagacgg
     7201 cgaccgcacg ctcgccgcgc ccgacacgcc gacgttgcgc gaccacctca accagcagct
     7261 cacgctcacc ggcatcggcg agcgcgaccg cgcgctggtg gcgctgctga tcgatgtgct
     7321 cgacgacgac ggctacctca cgcagccgct cgaggagatc gcggcgctgt tcgccgcgga
     7381 ggcgcaggtc gacctggagg agctgtcgat cgcgctcaag cacctgcaga acctggagcc
     7441 cgcgggcgtc ggcgcgcgct cgccggcgga gtgcctgctg ctgcagctgc gcgcgcacgc
     7501 cgggcacccg gcctgcgcgc tcgccagcga gatcgccgcc aggcacctcg cgctgctcgc
     7561 cgggcgcgac tacacgcggc tgcgcgcgct caccggcgcg caggaagcag acctgcgcgc
     7621 ggcgcagaag ctgatccaga gcttgaaccc gcaccccggc gcggcgttcg cgcgcgtcca
     7681 gacgcgctac atgatcccgg acgtgatcgt gcgaaagacg cgcaacgtct ggcgcgcgag
     7741 cctcaatccc gaggccatgc cgcgcctgcg catcaaccgc ctttacgccg atctcgcggg
     7801 cagcgcgcgc aacggctcga gcgcgctcgc ctcgcagctg caggaggcgc gctggctgat
     7861 caagaacgtg cagcagcgct tcgacaccat cctgcgcgtg tcgcaggcga tcgtcgaccg
     7921 gcagcgccac ttcctcgagc acggcgaggt ggcgatgcgg ccgatggtgc tgcgcgagat
     7981 cgccgagacg ctcggcctgc acgagagcac catctcgcgc gtcaccacgc aaaagttcat
     8041 ggcgacgccg cgtggcacct tcgagctgaa gtacttcttc agcagccagc tcgccaccga
     8101 cgccggcggc gcgacctcct cgaccgcgat ccgggcgctc atcaagcagc tggtgggggc
     8161 ggaagacgcg cacgcgccgc tttcggacgc gcgcatcgcg cagctcctcg gccagcaggg
     8221 catcgtcgtg gcgcgccgca cgatcgcgaa ataccgggaa tcattgcaga tcccgccggt
     8281 caaccagcgt aagagcctgt aggaaggagc gcatgaatct gaacctcagc ggccatcacc
     8341 tcgagatcac cccgtcattg cgcagctatg tggaggagaa gctggcgcgc gtcacgcgcc
     8401 atttcgacca cgtcatcgac gcgcacgtgg tcctgtcggt cgacaagctc aggcagaagg
     8461 ccgaggtgac gctgcatatg cgcggcaagg acatccactg cgcctccgag gagcaggacc
     8521 tgtacgccgc catcgacctg gtcaccgaca agctcgaccg ccaggtgctg aaatacaagt
     8581 ccaagcgcag tgccaagccg cacgaggcgc gcaagcgcgc tgacaatgtc tgaccgcagc
     8641 cccggcccgc gcagcggaca tcgccttggg ctataatccg cgccctctgc tgcgctgccg
     8701 cacgggcgcc ggatcatgag tctcgtcgcg aaactgcttc ctcccgccaa cgtcctgctc
     8761 gacctccagg tctcgagcaa gaagcgcctg ttcgagcagg tcgggctgat gttcgagaat
     8821 cacaacggca ttgcgcgcag cctggttttc gacagcctgt tcgcgcgcga gcgcctcggc
     8881 tccaccggcc tgggccaggg cgtcgccatc ccgcacggcc gcatcaaggg actgaaggac
     8941 gcactcggcg cgttcgtgcg gctcgcgcag ccggtgcctt tcgatgcgcc cgataccaag
     9001 ccggtgacgc tgctgttcgt gctgctggtg cccgagcagg ccaccgagaa gcacctgcag
     9061 attttgtcgg agctcgccca gatgttcagc gaccgcgcgc tgcgcgacgc catggccggc
     9121 gccgccgacg ccgccgccct gcaccagctg atcgccgcct ggcagcccga tgcagcaggt
     9181 caacgtcaag cggttgcatg aggtcaaccg cgaggcgctc gccctggcat gggtcgccgg
     9241 gcgcgatgcc gatccggccg tgcgccgcga gcccggtgcc gcggccgcgc tgatcggcca
     9301 cctcaacctc acccatccca atcgcatcca ggtcgcgggc gccgacgaag ccggcgcgct
     9361 cgagcgcctg gccgaagcgt tgttcgccgc cgcgccggcg gcggtgatcc tcgccgacgg
     9421 cgtcgccccg cccgccgcac tgctcgagcg cgccgagcgc gcgcgcacgc cgctgttcac
     9481 cacgccgctc gccgcagcgc gcgtcatcga gaagctcgcg cgctacctgg cgaaagcgct
     9541 cgccgagagc accgagcgcc acggcgtgtt catggacgtg ctcggcctgg gcgtgctgat
     9601 caccggcgat tcgggcgtcg gcaagagcga gctcggactg gagctgatca gccgcggcca
     9661 cggcctggtg gccgacgaca tggtggagat ctcgcgcctg gccgccgaca cgctggaggg
     9721 gcgctgcccg ccggtgctga aggacttcat cgaggtgcgc ggcctggggc tgctcgacgt
     9781 gcgcacgatc ttcggcgaga cggcggtgcg gccgaagatg aagctcaggc tgatcgcgca
     9841 cctggacagg cccgccgcgg tcgccgggcg cgacgtcgcc gagcgcctgc cgctcgccga
     9901 gcagtccgag gatatcctcg gcgtgacggt cagaaaggtg gtgattccgg tggccgccgg
     9961 gcgcaacctc gccgtgctgc tcgaggcggc ggtacgcaac tacatcctgc agctgcgcgg
    10021 catccattcc ggagcggaat tcatggcgcg ccagaagcgc gccatggacg gcgactgatc
    10081 ggcgccgcgt gcagatcatc ctggtgagcg ggctgtcggg cgcgggcaag agcatcgcgc
    10141 tcgacgtgct cgaggacgcg ggctattact gcgtcgacaa cctgccggcg acgctgctga
    10201 tggacgtggt gggctttctc gccgacgccg ggcatgagcg cgccgcgctc tcggtggatg
    10261 cgcgcagcgc ggcgctcgcc gcgctgccga agagcgttgc acgcctgcgc gccgacgggg
    10321 ccgactgccg cgtgctgttc ctggaggcaa acgcgccgac cctggtgcga cgcttctcgg
    10381 agacccgccg ccgccatcca ctgacggtgc agggcctcac gctcgcggag gccatcgagc
    10441 gcgagcgcac gctgatgggc gagatcgccg agctcgggca tcgcatcgac acgagcgagc
    10501 tgcagcccaa ggtgctgcgc aactggatcc gcgacctgct cgggctgggc gcgggcaggc
    10561 tggtggtgct gttcgagtcg ttcgcctttc gcgacggcct gccgctcgat gccgactggg
    10621 tgatcgatgc gcgcatgctg cccaacccgc actacgaccc gcagctgcgg ccgctcaccg
    10681 gccgcgacgc cgcggtgatc gcgtacctgg acggccagga ggcggtgcag cgcttcttcg
    10741 cggatgtgcg cgcgttcctc gggcggtggc tgcccgaggt ggtgaaggag aaccgcagct
    10801 atctgaccgt ggcgatcggc tgcacgggcg ggcggcatcg ctcggtttac ctggcggaga
    10861 ggcttgccgc ggcgttcgcg gccgagagcc gcgtactggt gcgccatcgg ggcctcgccc
    10921 gagagtgagc gacgccgatc tcgcggacct gccgctcttt gcgctgcaga cggtgctgtt
    10981 tcccggcggg cggctgccgc tgcgcatctt cgagcagcgc tacatggaca tggccaaggc
    11041 gtgcctgcgg gacgactcgc ccttcggcgt gtgcctgatc cgcgagggcg gcgaggtcgg
    11101 cgcgcccgcg gtgccggagg aggtcggttg cctcgcgcgc atcgtcgcct gggacatgcc
    11161 gcagctcggc gtgctgcagg tgcaggcgcg cggcgagcag cgctttcgca tccgcaagcg
    11221 ccgcgtgcag gcggacggac tcgtgcgcgc gagcattgaa ctgcttgcgg cggagctcga
    11281 cgcaaagctc gagcccgata ccgacctttg cgcgcgcgtc ctgcgcaaag cggtcgaatc
    11341 gggcggcgag gcgctggtcg agccgccgct gcgctacgcg tcggcagcct gggtgagcgc
    11401 tcgtcttgcc gagctcctgc cgctgtccct cgacgccaag cagcgcctgc tggagatgaa
    11461 cgacgcgcgc gaccgcctcg agatcctgcg ccgcctaatc agctcggccg gccagtagcg
    11521 aacgcagcaa tcggcgcacg ggcgccggga ccgcggcgcc cgtggcttcc gccatatcga
    11581 tccagagccg gttcgcgccg gccggggtcg cgccgcgcag cgcgcagagc cagggctggg
    11641 cgcgcagccg gaaatgcgtg aagccgtgct cgagcagcgc cagcttgcgg ccgcgcggcg
    11701 ccgccggcag gcgcgcctcg ggaaacgtcc acaacccgcc ccacaggccc gcgcccggcc
    11761 ggcgttccag cagcacctcg gcaccgcgca cgtagacgaa ccagtgggcg cgcttcagcg
    11821 gcaggggctt gcgcgcgcgc gctgcgggca gctcggcgat gcgtccctcg cgccgcgcca
    11881 cgcagcccgt cgccaccggg cagcgctcgc aggccggccg cgtgcgcgtg cacaccagcg
    11941 cgcccagatc catcaacgct tgcgtgtagg cgcgcacgcc cttgcgcggc aggagctctt
    12001 cagccaggtc ccactgggcc ttctcgccct gcacgccgaa gcggcgcgcg agcacccgct
    12061 ttacgttgcc gtcgaggatc gccgcgcgcc ggccgaaggc gaacaccgca atggccgcgg
    12121 cggtcgatcg ccccaccccg ggcagggctt gcagccccgc cgcgtcgcgc ggaaatcccc
    12181 cacgggccac cacggcacgc gcggccgcgt gcaggttgcg cccgcgcgcg tagtagccca
    12241 ggccgctcca gcgccgaagc acctcgtcct cggtcgccga cgccagcacc gcaacccccg
    12301 ggaagcgcgc aaggaagcgc cggtagtagg gaatcaccgt ctccacctgc gtctgctgga
    12361 gcatcacttc ggcgagccac acgcggtaag gatcgcgcgt accctgccac ggcaggtcgt
    12421 ggcggccgtg gcggcgctgc caggcgatga gccggggggc gaattgcctc atgcgcaatt
    12481 gtatgcacgg tatgattgcc gccacccatg ctggccttcg tcctgcgccg cctcgggcaa
    12541 gcggtcctcg tcatgctcgc cgtagggctg attgcgtttg cgctgttccg cttcgtcggc
    12601 gaccccgtgc tgttcatgct cgggcaggac gcgacgccgg aggaccgcga gcgcatcacg
    12661 cggctgctcg gcctggacca gccgttctac gtgcagtacg caaagttcgt cggccgcgcg
    12721 gtgcagggcg aattcggcct cagcctgcgc caggtgcagc ccgtatcgaa gctgctggtg
    12781 gagcgcctgc cggcgacgct ggaactgtcg ttcgtcgccg ccgccttcgc gctcgtcgcc
    12841 ggcatcgcca tgggcgtata cacggcgctc cggcgccaca cctggctgtc gcagctgttg
    12901 ctcgcggttt cgctgctcgg ggtatcgttg cccacgttcc tcatcggtat cctgctgatc
    12961 ctcgtgttcg cggtgctgct cggctggctg ccctcgttcg gccgcggcga gacggtgatg
    13021 atcggctggt ggagcacggg cctgctgacc tggagcggca tcaaggcgct gatcctgccc
    13081 gcggtcacgc tgggcctgtt tcagatgacg ctgatccagc gcctggtgcg cgccgagatg
    13141 ctggaagtcc tgcgcaccga ctacatcaag ttcgcgcgcg cgcgcggcct taccgaccgc
    13201 gcggtgcatt tcgggcatgc gctaaagaac acgctggtgc cggtgatcac catcaccggg
    13261 ctgcagctcg gtgccatcat cgccttcgcc atcatcaccg agaccgtgtt ccagtggccg
    13321 ggcatgggcc tgctcttcat ccaggcggtg ggcttcgccg acgtgccggt gatggccgcg
    13381 tacctgtgcc tgatcgcgct gtttttcgtc gtcatcaacc ttgccgtgga cctgctctac
    13441 tacgcggtcg acccgcgcct gcgcgtcgag cgcagcgcca tggcgcagtg aggagcctat
    13501 gaagaacgat cccctcgaga tgctgcggct gtaccacgcc gacctggtcc acatccgccg
    13561 cgacatccac gctcaccccg agctcggctt cgaggagacg cgcacctcgc aactcgtcgc
    13621 cgagcggctc gccgactggg gtatcgaggc gcaccggggc ctggcgaaga ccggcgtcgt
    13681 cggcgtcgtg aagggcagga aggacggcgg gcgcgccatc gggctgcgcg ccgacatgga
    13741 ctgcctgccg ctgcacgaga ccggcgacgc gccgcacaag tcgaagttcg ccgggcgcat
    13801 gcacgcctgc ggccacgacg gccacaccac gatgctgctc ggcgcggcgc gctacctcgc
    13861 gcagacgcgc aacttcgacg gcacggtgca cctcatcttc cagccggccg aggaaggtgg
    13921 cggcggcggg cgcgtcatgg tcgaggaagg cctgttcgag aagtttccct gcgaggcggt
    13981 ttacgcgctg cacaactggc cgggcctgcc gtccggcaag atcgccgtgc gccccggccc
    14041 catgctggcg gcgaccgacg agatcaagct caaggtgcgc ggcaagggcg gccacgccgc
    14101 gctgccgcac ctcgccaccg atcccgtggt gatcgccgcc cacatcatca ccgcgctgca
    14161 gaccattgcc agccgcaacg ccaatccggt ggacgcggtc gtggtgtcgc tgtgctcgat
    14221 gcagacgagc cagctcggcg ccttcaacat catcccgacg cacgtcgagc tggtcggcac
    14281 ggtgcgcacc ttcaccccgg agatgcgcga tctggccgag cggcgcatcc gcgagatcgc
    14341 cgacgccgtg gcgctcggcc tgggcggcag cgccgagatc aactaccggc gcggctaccc
    14401 ggccaccgtc aacaccgaga aggaagcgca cttcgcggcg caggtcggcg agaaggtgtt
    14461 cggaaaagcg aacgtcatca ccgaccatga gccgaccatg ggcggcgagg acttcgccta
    14521 catgctgcag gccaagcccg gcgcctatgt gttcctcggc cagggcggcg cccagggcgg
    14581 ctgcttcctg cacaacccga cctacgattt caacgacgag gtgatcccgc tcggcgccgg
    14641 ctacctcgcg gcgctggccg aggccgccct gccactgaag taaaccaagg agaccgcacc
    14701 atgaagctca agcacctgat cgcggccgcg ctcgtcgcgg tcgtgctgcc ggcgcacgcc
    14761 gcaacgttgc gctgggcggc gcagaacgac attctcacgc tcgatccgca ttcccagaac
    14821 cacgccacca ccagctcgct gctgcagcac gcctatgagg gcctgacgcg ctacgccaag
    14881 gactacagca tcgagccggc gctcgccacc ggctggcagc agctctcgcc gacgcacctg
    14941 cgcttcacgc tgcgcaaagg ggtcaagttc cacgacggca gcccgttcac ggccgaggac
    15001 gtggtgttct cctacggccg catcctgcag ccgcagggca cgatgcagat ctacgtcggc
    15061 ggcgtgaagg aagtaaagaa ggtcgacgac tacacggtgg acttcatgct cgccgggccg
    15121 aacccggtgc tcctcaggaa catcgccgac ttccgcatca tgagcaagac ctgggcgatg
    15181 aagcacaagt ccgagaacgt gcagaactac gtcgccaagg aggagtccta cgcctcgcgc
    15241 aacaccaacg gcaccggccc ctacatcatc aagggctggg agcccgacaa gcgcatcgtg
    15301 atgaccgcca accccgagtg gtggggcaag cgcgagggca atgtcaccga cgtcgtctac
    15361 acgccgatca aggccgatgc cacgaggatc gcggcgctgc tctcgggcga cgtcgacatg
    15421 gtcaccgacc tgccgacgca ggacgtgacg cgcctgcgga gcgaggcgaa gctcaaggtg
    15481 ctcgacggtc acgaggtgcg caccatcttc atcggcatgg accagcactc cgacgagctc
    15541 aagtactcca gcgtcaaggg caagaacccg ttcaaggacg tgcgcgtgag gaaggcactc
    15601 aacctcgcag tggatcgcga ggccatccgc cgcgtcacca tgcgcgggct gtccatcccg
    15661 gcggcgatca tggtcgcccc cggcgtgcac gggcacgcca aggacatcga ccaggcgctc
    15721 gcgctcgacg tggaaggggc gaagaagctg ctggccgacg ccggctacgg aagcggcttc
    15781 gagttcggcc tggactgccc caacaaccgc tacgtgaacg acgaggaaat ctgccaggcg
    15841 ctggtcggca tgtgggcgcg gatcggcctg aaggcgaagc tgaacgcgca gccgatggcc
    15901 accttcatcc agaagatcca gaagttcgac cacgacgcct acatgctggg ctggggcgtg
    15961 ccgaacttcg acgccaacta cacgctgcag tcgctggtgc gcaccaagac caccggcgcg
    16021 gacggcagct tcaacctggg ccgcatcagc gacgcgaaga tcgacgccct ggtcgacgcg
    16081 atcaagaccg agctcgacac cgccaagcgc gacgccatgc tgcgcgaggc gctggtgctg
    16141 acgcgcgaca accactacta cgtcccgctg caccaccagt tgcggccgtg ggcgatgaag
    16201 aagaacgtca ctgcggtgca taacgcggtc gaccggcacg agtctcgtta cacccgcgtc
    16261 gagtaagcag ggtgagctgg ctgcggcgcg cctgggacca tgacctggcg tggagcttcc
    16321 ggcattcgcc ggtggctctc gccgcggccg tgctcacggc gctgtgcgtg gggggcgccc
    16381 tgctcgcccc ctggctcgcg ccgcacgacc ccttcaacag cgcgacgctg aacctctccg
    16441 agggcttcac gccgccggcg tggagcgaca agggcagcgc caagtacctg ctcggcaccg
    16501 acgaccaggg ccgcgacgtg ctctcgacca tcatgtacgg cgcgcgcatc tcgctcggcg
    16561 tggggctcgc ctcggtgctg ttttcggcgc tgctcggggt gtcgctcggg ctcgtctcgg
    16621 gctacctcgg cggacggctc gacgcattca tcatgcgcat cgccgacatc cagctgtcct
    16681 tcccggcgat cctggtggcg ctgctgatcg acggcatcgc gcgcatcgcg ctgccacggg
    16741 agctgcacga cgcgctcgcc gtctacgtct tagtgctggc gatcgggctc tcgggctggg
    16801 tgcagtacgc gcgcacggtg cgcggctcga cgctggtgga gaaggggaag gagtacgtgc
    16861 aggccgcgcg cgtcatcggc cggcacccgc tcgccgtcat gctgcaccac gtgctgccca
    16921 acgtcatggg gccggtgctg gtgatcgcca ccatccacgt cgccaccgcc atcatcaccg
    16981 aggcgacgct gtcgttcctc ggggtgggcg tgcccccgac gcagccctcg ctcggcacgc
    17041 tggtgcgcat cggcaacgat ttcctgctgt cgggcgagtg gtggatcgcg ctgtttcccg
    17101 gcgccgccct ggtcatcctg gtgctggcgg tgaacctgct cggcgactgg ctgcgcgacg
    17161 cgctcaaccc gaagctccgg tgagcgcgcc gctcctcgag gtccgcgacc tgcgggtcga
    17221 attccccacg cgccgcggca cgctggtcgc ggtggacggg gtgtccttcg ccatcgcccc
    17281 gggcgaagtg ctcggcgtgg tgggcgagtc gggcgcgggc aagtcgctca ccggcacggc
    17341 gatcatcggc ctgctcgagc cgcccgggcg catcgccgcg ggcgagatcc gcctcgaggg
    17401 ccggcgcatc gacgagctgc cgcccggcga gatgcgcaag ctgcgcggcc ggcgtatcgg
    17461 cgcgatcttc caggacccgc tcacctcgct caacccgctg tataccgtgg gcgatcagct
    17521 ggtcgagacc atcctcacgc acctcgacat gagccgcgac gaagcgcggc gccgtgcgct
    17581 gcacctgctc gccgaggtcg gcatcccggc gcccgatgcg cgcatcgacc agtacccgca
    17641 ccagttctcc ggcggcatgc gccagcgcgt ggtcattgcg ctggcgctcg ccgccgagcc
    17701 gcggctgatc atcgccgacg agccgaccac cgcgctcgac gtctcgatcc aggcgcagat
    17761 catcacgctg ctcaagcgcc tggcgcgcga ccaccgcacc gccgtgatgc tgatcacgca
    17821 cgacatgggc gtgatcgccg aaaccgccga ccgcgtcgcg gtgatgtacg cggggcgcat
    17881 cgcggagatc gggccggtgc gcgaggtgct gcaggcgccg cgccatccct acacggcggg
    17941 cctgatgggc tcgatcccga agctcggcac gtctcgtcgc cacgcccgcc tggtgcagat
    18001 cgatggcgcc atgccgcgcc tcaccgcgat cccggcgggc tgcgcctacc atccgcgctg
    18061 ccccagtcgc tttgcgcgtt gcgacgcgga gcggcccgag ctgctgcccg tcggcaacgc
    18121 ccgcgcgtcc tgctggctct atggatagcc ggccgctgct gcgcctcgag ggcgcgagcc
    18181 gggacttcga cgtctcgcgc ccctggctca accgcctgct cgagggcgcg ccgcgccagc
    18241 tgctgcgcgc ggtggacgac gtctccttcg aggtggcgcg cggcgagacg ctcgcgctgg
    18301 tgggcgagtc gggctgcggc aagtccaccg tcgcgcgcct catcgtcggc ctgtatccgc
    18361 ttacgcgcgg acgcatcgag ttcgacggcg ccgacctgtc cacggccggc gcgcaccggc
    18421 agcggcggcg catgcagatg atcttccagg acccctacgc cagcctcaac ccgcgctggc
    18481 gcgtgcgcga catcgtcgcc gagccgatcc gcgtgctcgg gctcaccgcc tcaaaggacg
    18541 ccgagcgcgc gcgcgtcgcc gagctcctgc gccaggtggg cctcgcgccc gaggacggcg
    18601 agaagtaccc gcacgagttc tcgggcggac agcgccagcg catctcgatc gcgcgcgcgc
    18661 tctccggcaa ccccgagttc ctggtgtgcg acgagcccac ctcggcgctt gacgtctcgg
    18721 tgcaggcgca gatcctcaac ctgatgaccg agctgcaggc gcgcctcggc ctcacctacc
    18781 tcttcatctc gcacaacctc gccgtggtgt cgcacgtcgc cgaccgcgtg ggcgtgatgt
    18841 acctcggccg cgtggtcgag atcgcgcccg cggcgcagct gttcgccgcg ccgcgccatc
    18901 cctatacgcg catgctgatg gacgcgatcc cggaccttga gatgagcggc aaggcgcgca
    18961 cgcccgtggc tggcgaggta cccaatccgc tcgcgccgcc cgccggctgc gccttccacc
    19021 cgcgctgccc gcacgccgat gcgcgctgcc gctcggagcg gccgcggctg cgtcccgccg
    19081 ccggcggcgc ggtggcctgc cacgcggtgg aggaagggcg cctctagcgc ctgatcaggc
    19141 cgcgcagctt gtcttccagc ttcttctcga tcttttcctc ggccttcttc gctttctcct
    19201 tgaccttcgc cttggcggcg tcgccggcaa gcgcgcggta gtcgatctcg tacttgggcg
    19261 cgtcgaacgg gccggtgagc ttcaccggga tggtgaggcc cttcaggtgc tcgagccccg
    19321 cgccgccctg tccctcggcg ctcgccacca ccgacgcctt ggccaggtag tccagccgcg
    19381 agttgccgat gtcgatgtcg cccgccccgc ccacgcgcag cgccggcgcg cgcacatcca
    19441 ggtcgtcgtt gcgcgccacg ccgtccttga tgacgaagct cgcgctcatg gccgagaagt
    19501 cggtgcgctt ggaggtgtcc gagggctgct cctgcgccga cttggagccg aaggcggcgc
    19561 gcgtcttgcg cagcacctcg gcgaggttga tgcccttgac cgccccgtcc ttcagctcga
    19621 cgcgcgcggt gccggcgagg gacttcttca gggcgttcac ggtgggcccg gacgccgtga
    19681 cgtcgaccgc cacgtcgccc cgcccttcca ccgcgtcgcg cgccatcagg tccttcacca
    19741 gcgggccgat cgcgacgccg cgcagcgtct ccttcaccgc gatgcggttg ccgcgcgcga
    19801 tgagcgtcat cgcgccttcg agaccgcctt cgtacagctt cgccgaatgc ggggcgatgt
    19861 cggcgcgccc gtttgccgcg cgcagctcgg ccttcaggtc cgagaccttc aggcccataa
    19921 gctgcatcca gccgatggag agcttgccgc tggcgcgcaa gccctcgagc gcgctcaggt
    19981 cgaccggcac gtccaccgcc accttcggcc ccgcgctcgg cgcgggcttg gcgtccttct
    20041 gcgccacgag gtagcggtcg aggtcgatgc ggtccaccgc ggcgtccacc cgatagctcg
    20101 gcgcgtcgaa gccggccatg ccgagcttca tcttcaccgt ggtgtcgtcg aacttcgcca
    20161 ccaggtccgc gtccagcgcc tccttggcga ggcgcgcggt cgcgtcgccc gtgaccgtga
    20221 gggcgatgcg cgtgaactcg tccgccgtac cgctcacgcc caggttcatc ccggtgagcg
    20281 agtattcccg gcgctcgagc gcgaggcggt aggtgccggc gagatccatt ttcaggtcga
    20341 tgagcggctt cttgccgcgc acgcgcgcgc caagcgccag cgtccccggc acgtcgtcgg
    20401 cgatgcgccc ggtggtgagc gtcacgtcgt cgagctccag gcgctggccg ctgccctcgt
    20461 cgagataggc gaagcgcgca tcgcgcagcc tgatgcccgc gacgtcgaac gccaccttcc
    20521 gcggcgcggc gccgcgcgcc gccggcgcct tgcccgcgcc gccgccggcg cccagcaggt
    20581 cctcgaagtc gaacttgccg ttcttcgccc ggatgacgct ggcgcgcagg ccggtgagcg
    20641 tcacctcgtc gacctgcacc tggcccgaca ggagcggcag cagcgccacc gcgacctgcg
    20701 cgccgtcgat cgcggcgaag tcgtgctcgc tcgcgcgccg cgacaacgtc accttgccca
    20761 ccttggcgcc gatcttgggc cagagggcga gcgcgatgtc gccgtggaac ttcaacgtgc
    20821 ggccggtggc ctccttcaca cgcttctcga tctcgccctt gtaggcgttg gggtcgaacg
    20881 tggcgacgat gacggcgaca ccgacgagcg ccagcacgat cacgccgagc aaggagtaca
    20941 ggacgtactt cagccatttc atgcgggttt cctcccggcg atgccgaagg ggacgtggcc
    21001 ggtggccacg tgcctgccgg cagactcgac gtggccgcgc tggtcgtcga agtagaaatc
    21061 gggctcgaac gcgccgagga actcgccctt gtcgagcccg ccgaggaaca gcgcttcgtc
    21121 gaccgagatt ttccagtgca tcagggtctt caccgcgcgc tcgtgcgccg gggcgctccg
    21181 cgcggtcacc agcgccgtgc gcaggcgcat cggggccttc gccgtctgca ggcggtgcag
    21241 cgcctcgagc agcggcttga acggccccgg cggcagcggc gtattcacgc ggtcggtctc
    21301 gtggcgcgtg aacgcctcca gcccgtcggc ctggtagacg cgctcggcct catcggaaaa
    21361 cagcaccgcg tcgccgtcga acgcgatccg cacctcgtcg gcgtgccggc ttgctgcctg
    21421 cgccgactcg ggcaccacgc gcgcggcggg aatccgcgcg tcgagcgcgg ccatcacgtc
    21481 gcgctcgttg gccgagagga acaggttggc gcgcagcgca tcgagatacc ggtaggggtc
    21541 gcgcccgcgc gtgaacacgc cgcgctcgac gctcaggccc tcgcgcttgg ccgagcggaa
    21601 caccctgagg ccgcttaccg gatcattctt ggagaggatc accacctcca cgcgctgagc
    21661 ctccggggta ttgaaggcga gcagcttctg caccagcggg aaggcgaccc cgcgcttggc
    21721 cggcacgtcg aggcgcccaa gctgcagcgt gacgtacgct ggctcgccgt cgcggtcgaa
    21781 gacgcggttc tcctcctcga agtcgaacag cgcccgggag gagatggcga ccaccagttt
    21841 tccgtcaaag gttacaggca tggcatatgg agcgggtgat gggaatcgaa cccacgttca
    21901 cagcttggga agctgttgtc ttgccattag acgacacccg cgggttgcgg ccaagtgtat
    21961 catttgccct atgattcaag tcactgtgaa cggcgcggcg cggcgcttcg agcgccccct
    22021 ggcggtgaac gagctggtgg ccgagctcgc gctttcgggc aagaagatcg cggtggaaag
    22081 aaacggcgag atcgtgccga agagcgcgca cggctcgacc ctggtcgccg acggcgaccg
    22141 cctggagatc gtggtcgcgg tgggaggcgg atgagcgaca ggctcctcgt tgccggcaag
    22201 gcctacggct cgcggctgct catcggcacc ggcaagtaca aggacttcgc gcaaacgcgc
    22261 gccgcggtcg aggcgagcgg cgccgagatc gtgaccgtcg ccatccggcg caccaacatc
    22321 ggccagaacg ccaacgagcc gagcctgctc gaggcgctgc cgccctcgaa gttcacctac
    22381 ctgccgaaca ccgccggctg ctacagcgcc gaggacgcgg tgcgcacgct acgcctcgcg
    22441 cgcgagctgc tcgacggcca cgacctggtg aagctcgaag tgctgggcga cccgcacacg
    22501 ctgtatccga acatgccgga gacgctgaag gcggcgggcg agctggtgcg ggacggcttc
    22561 aaggtcatgg tgtactgctc ggacgacccg atccaggcga agatcctcga agacgcgggc
    22621 tgcgttgcca tcatgccgct cgcctcgctg atcggctcgg gcatgggcat cctcaatccc
    22681 tggcaggtac agctggtgcg cgacgccgtg aagctgccgg tgatcgtcga tgccggagtg
    22741 ggcaccgcct cggatgccgc cattgccatg gaattgggct gtgacggggt gctcatgaac
    22801 accgcagtcg ccgcggcgaa ggatcctgtc atgatggccc gcgccatgaa gaaggcggtc
    22861 gaagccgggc gcgaggcctg gctcgccggg cgcatgccga agaagctcta tacggcggcg
    22921 ccctcttcac ccaccgaagg gacgatcgcg ccttcccgca gcaaggcggc ttgaagcgcg
    22981 gcatccgcag ctacgtgctg cgccagggcc gcaccacgcc cgcgcagcag cgggcgctcg
    23041 atgagctgtt cacggtctac ggcatcccct tccgccaggc gctgctcgag ccgcgtggca
    23101 agccgctcgt cctcgagatc ggctcgggca tgggcgagac taccgcggcg atcgccgccg
    23161 cgcagcccgg cagcgacttc attgccgtgg aagtacatgg ccccggcgtc ggcagcctgc
    23221 tcaggcgcat cgacgagcag ggcctgaaga acctgcgcgt catccggcac gacgccatcg
    23281 aggtgctcga gcacatgatc gccgacgcct cgctcgcagg cattcacctg ttctttcccg
    23341 acccctggcc caagaagcgc caccacaagc gccgcctggt gcagccggcg ttcgccaccc
    23401 tggccgcacg caagctcgcg ccgggcgggt acctgcacgc cgcgaccgac tggccgcagt
    23461 acgccgagca gatggcggag gttctcgatg ccgagccgct gctcacgcgc gccgcggcgc
    23521 gcgagcgtcc cgccaccaag ttcgagcaac gcgggatgaa gctcggccac gaggtgcgcg
    23581 acctcgtcta ccggaggcgc tgaatcagct tttcaattcg tcgagcttgc gccggtcgcg
    23641 cttggtcggc cggcccttga tcgtgcgcgc cggctcggcg ctatggcggc gcaggtcggc
    23701 gcgccgctcg cgctcggcgc ggctcgcttc ggactccgca tagagcttgc gtgcctgcgc
    23761 cgccggtccg cgcttgtcgg aaagcgccgt gacctcgacc tgccagacgg attcccccac
    23821 ccgaatttcc agttggtcgc cgaccttcag cgcatgggcg ggcttggtct tttcctcgtg
    23881 cagcttgacc cggcccgacg cgatcgccag ctgcgccagg ccgcgcgtct tgaaccagcg
    23941 cgcggcccac agccacttgt ccaggcgcat cgtcagtcga ggaacatctg caatagctcg
    24001 ttgaggaaca gccttccccg ccgcgtcggg cggatgcgcg ccgggtcgtt ctccagcagg
    24061 cccttctctt cggcggcggc gagcttgcgc gcgacagcgg caagcggcag cccggtgcgc
    24121 tcctcgaagc gcaccttcgg cacgccctgc accaggcgca gggcgttcag catgaactcg
    24181 aacggcagct cgcgcggcgc gatcggcttg tccacggcgc ttcccgcctg cgccaggtac
    24241 tcgcgcggct gcttggcgcg ctcgtggcgc gcgacgtggt cggggaacga gagcttgccg
    24301 tgcgcgcccg cgccgatgcc caggtaatcc ccgaactccc agtagttcag gttgtgccgg
    24361 cagcgtcgtc ccgg
//
LOCUS       DBNHKE010000176         5330 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1353162, whole genome shotgun sequence.
ACCESSION   DBNHKE010000176 DBNHKE010000000
VERSION     DBNHKE010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1353162"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="AC16O9_12170"
     CDS             <1..1623
                     /locus_tag="AC16O9_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6517408.1"
                     /translation="VQAFVGTAQRLYVPAVAALLKAPWLVVAAALALLTGFGALFLQF
                     TGKQAFLPAMDDGRVQVQVYTDPGGALQQMDDTVRLLEKLARAQGGVEGISVIAGGSI
                     FGRTQRERPNRSTLGIQLVPVDERGKSVEQWVSGFSRAVAAEQLAGVKVLARPSGIRG
                     VRVSRADEDISVRVMGPELAVLASIGDRLLERLRDRPGLRNAKHSAEEQRQEFAVRLD
                     RTRAAELGVDVAQVGRALRVALDGVVVSNFIEADRAYDVRVRLPRGTIDSPAAMEAIL
                     LFGAQGGRPAVYLGDVARAELVVAPTEIRRENQRRIVEVSAALTGELPLGEVVRDLRR
                     DLRGVELPPGYSLYFGGAYDSLRKGNVLVATLASLALFLVFVVMAVQYESLRNPAIIM
                     LGVPFALIGVVIGLVATGLPLSMPVWLGIIMLIGVVVNNAIVLVEYIELLRQRGRALV
                     EAIVESARLRLRPILMTTLTTVVGMSPLALGVGEGSEMLRPLAVCMVFGLLFSMFVSL
                     LLIPSIYLLVHRPRARPGLTRSPTPSANAAGP"
     gene            complement(1580..2128)
                     /locus_tag="AC16O9_12175"
     CDS             complement(1580..2128)
                     /locus_tag="AC16O9_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6517409.1"
                     /translation="MRLLPSDPLKRGRVKLALLGLFFAAPFALAWLAYWLDWAPGTTS
                     NYGELISPPLPLAGAPLERLRGKWVLVTLDGAACDAYCERKLYFMRQLRRAQGKNQER
                     VERLWLLTSGTRPGPKLLQAIEGTHVEPAGTLAARFPGAVSDHIYVVDPLGNLMMRYP
                     REPDPSRMLKDLQRLLKVSGIG"
     gene            complement(2109..2753)
                     /locus_tag="AC16O9_12180"
     CDS             complement(2109..2753)
                     /locus_tag="AC16O9_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR6517410.1"
                     /translation="MISGYSFRPRAWAFALAVAGCAAGIALGNWQAGRAAEKRAAAVA
                     EKRLQLRGEFLERYTVLLDNKVFRGRVGYHVVQPLKLADGRHVLVNRGWIAAPAHRER
                     LPQIRTPAGAHEVEGRVLEHFPRAYDPTDAPPSGRVWQNVELTTFAAWSGLRLEPYVL
                     EQHSPFPDGLTRNWPPPDSGADKNESYALQWYTLAALAVVLFLVLSVRRAPPAV"
     gene            2752..2946
                     /locus_tag="AC16O9_12185"
     CDS             2752..2946
                     /locus_tag="AC16O9_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR6517411.1"
                     /translation="MRYLVLAVFVLIVLSLASGLYFVFHDRSDSKRAVKALAVRVGLS
                     VGLFVFLMVAYYFGFVPARL"
     gene            complement(3095..3958)
                     /locus_tag="AC16O9_12190"
     CDS             complement(3095..3958)
                     /locus_tag="AC16O9_12190"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6517412.1"
                     /translation="MAQGATTQRQHYYVPQPMYWPLLGSMSLFLMALGGVFVMNASKG
                     GWVPIAAGAVLLVYMMFRWFGDVIAESEGGKYHGWEDVSFRWGMSWFIFSEVMFFAAF
                     FGALFYIRVLSVPDLASLEHNELLWPDFKSTWPSAGPGFEERFSPMGAWGLPAINTLL
                     LLSSGVTVTVAHWALKEGNRAMLNLFLFLTIALGFIFLGVQAYEYGHAYADLNLKLTT
                     GAYGSTFFMLTGFHGFHVTVGAIMLSVIFARCLKGHFKPDHHFAFEAVAWYWHFVDVV
                     WLGLFIFVYWL"
     gene            complement(3994..4161)
                     /locus_tag="AC16O9_12195"
     CDS             complement(3994..4161)
                     /locus_tag="AC16O9_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HIR6517413.1"
                     /translation="MKAAVKTVLSGMIGIRRKADHESQRIDPVHVIVAGVALAALLVF
                     TLVTIVRIVAG"
     gene            complement(4158..4706)
                     /locus_tag="AC16O9_12200"
     CDS             complement(4158..4706)
                     /locus_tag="AC16O9_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR6517414.1"
                     /translation="MSEPQAPRREDNRRMLLRLMVVAVAMFGFGYALVPFYDAICSAL
                     GVNDFLKADVRPANTQIDATRTVTVELDANAHDLPWSFRPLVRHIRVHPGEVATVEYE
                     IANVRSAPVTAQAVPSYGPQRAGEFFRKFECFCFTQQTLAAGETRRFPVVFVVDPKLP
                     RDVNTITLSYTFFEVAGRGGKS"
     gene            complement(4703..4804)
                     /locus_tag="AC16O9_12205"
     CDS             complement(4703..4804)
                     /locus_tag="AC16O9_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HIR6517415.1"
                     /translation="MALSKNAKTGLILATIALAWFAGLILKYWIMDV"
     gene            complement(4807..>5330)
                     /locus_tag="AC16O9_12210"
     CDS             complement(4807..>5330)
                     /locus_tag="AC16O9_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR6517416.1"
                     /translation="CGFLFLFTLGGFTGLVLSLVPIDIQLHDTYYVVAHFHYVMVAGA
                     LFAAFGGVYFWLPKWTGRMYDETLGKWHFWLTMIFFNVTFFVQHFSGLAGMPRRIPDY
                     PLMFETWNKISSIGAFGFGLSQLLFLYVVLKCIRSGPKAADKPWEGADSLEWTHLPTP
                     APYHTFDPQPDLR"
BASE COUNT          842 a         1800 c         1880 g          808 t
ORIGIN      
        1 gtgcaggcgt tcgtcggtac cgcgcagcgc ctgtacgtgc ccgccgtggc cgcgctgctc
       61 aaggcgccct ggctggtggt ggccgccgcg ctggcgctgc tcaccgggtt cggcgcgctc
      121 ttcctccagt tcaccggcaa gcaggcgttc ctgccggcga tggacgacgg ccgcgtgcag
      181 gtgcaggtct acaccgatcc cggcggcgcg ctccagcaga tggacgacac ggtgcgcctg
      241 ctggagaagc tggcgcgcgc ccagggcggc gtcgagggca tctcggtgat cgccggcggc
      301 tcgatcttcg ggcgcacgca gcgcgagcgc cccaaccgca gcacgctcgg catccagctg
      361 gtgccggtcg atgagcgcgg aaagagcgtg gagcaatggg tgagcggctt ctcgcgcgcg
      421 gtggccgccg agcagctcgc cggcgtcaag gtgctcgcgc gcccttcggg catccgcggc
      481 gtgcgcgtgt cgcgcgccga cgaggacatc agcgtgcgcg tgatgggacc cgagctcgcg
      541 gtgctggcct cgatcggcga ccggctgctc gagcgcctgc gcgaccgccc ggggctgagg
      601 aacgccaagc actcggccga agagcagcgc caggagttcg cggtgcgcct ggaccgcacg
      661 cgcgccgccg agctcggcgt ggacgtggcg caggtcgggc gcgcgctgcg cgtggcgctc
      721 gacggcgtcg tggtgagcaa cttcatcgag gccgaccgcg cctacgacgt gcgcgtgcgc
      781 ctgccgcggg gcacgatcga cagcccggcg gcgatggagg cgatcctgct gttcggcgcg
      841 cagggcggcc gccccgccgt gtacctgggc gacgtggcgc gcgccgagct ggtggtggcg
      901 cccaccgaaa tccggcgcga gaaccagcgc cgcatcgtcg aggtgagcgc cgcgctcacc
      961 ggcgagctgc cactgggcga ggtggtgcgc gacctgcgcc gggacctgcg cggcgtcgag
     1021 ctgccgccgg gatactcgct gtatttcggc ggcgcctacg acagcctgcg caagggcaac
     1081 gtgctggtcg ccacgctcgc ctcgctcgcg ctgttcctgg tcttcgtggt gatggcggtg
     1141 cagtacgagt cgctgcgcaa tcccgccatc atcatgctcg gcgtgccgtt cgcgctgatc
     1201 ggcgtggtga tcggcctcgt cgccaccggg ctgccgctct cgatgccggt atggctcggc
     1261 atcatcatgc tgatcggcgt ggtggtgaac aacgccatcg tgctcgtcga gtacatcgag
     1321 ctgctgcgcc agcgtggccg cgcgctcgtc gaggcgatcg tcgaatcggc gcgcctgcgc
     1381 ctgcggccga tcctgatgac cacgctcacc acggtggtcg gcatgtcgcc gctggcgctc
     1441 ggcgtcggcg agggctcgga gatgctgcgg ccgctggccg tgtgcatggt gttcgggctg
     1501 ctgttctcga tgttcgtcag cctgctgctc atcccgtcga tttacctgct ggtgcatcgg
     1561 ccgcgcgcgc ggcccggcct cacccgatcc ccgacacctt cagcaaacgc tgcaggtcct
     1621 tgagcatgcg cgacggatcc ggctcgcgcg ggtagcgcat catcaggttg ccgaggggat
     1681 cgacgacgta gatatggtcg ctgaccgcgc ccgggaagcg cgccgcaagc gtgcccgcgg
     1741 gctcgacgtg cgtgccctcg atcgcctgca acagcttcgg tcccggccgc gtgccgctcg
     1801 tgagcagcca cagccgctcg acgcgctcct ggttcttgcc ctgcgcgcgg cgcagctggc
     1861 gcatgaagta gagcttgcgc tcgcagtagg catcgcaggc cgcgccgtcg agcgtgacca
     1921 gcacccactt gccgcgcagc cgctcgagcg gcgcaccggc gagcggcagc ggcgggctga
     1981 tcagctcgcc gtagttggag gtcgtccccg gcgcccagtc gagccagtac gcgagccacg
     2041 ccagggcgaa cggcgcggcg aagaacagcc cgagcagcgc gagcttgacg cggccgcgct
     2101 tcagcgggtc agacggcagg aggcgcacgg cgcacggaca gcaccaggaa cagcaccacg
     2161 gcgagcgcgg cgagcgtgta ccactgcagc gcgtagctct cgttcttgtc ggcgcccgag
     2221 tcgggcggcg gccagttgcg cgtgaggccg tccggaaacg gcgaatgctg ctcgagcacg
     2281 tagggctcga gcctgagccc cgaccaggcg gcgaaggtcg tcagctcgac gttctgccac
     2341 acgcgccccg agggcggcgc gtccgtaggg tcgtaggcac gcgggaagtg ctccagcacg
     2401 cgcccctcga cctcgtgcgc cccggccggg gtgcggatct gcggcagccg ctcgcggtgc
     2461 gcgggcgccg cgatccagcc gcggttgacg agcacgtgcc ggccgtcggc cagcttcagc
     2521 ggctgcacca cgtggtagcc cacgcggccg cgaaacacct tgttgtcgag gagcaccgtg
     2581 tagcgctcga ggaactcgcc gcgcagctgc agccgcttct cggccaccgc cgcggcgcgc
     2641 ttctccgccg cccggcccgc ctgccagttg ccgagcgcga tgcccgcggc gcagccggcc
     2701 acggcgaggg cgaaggccca ggcgcgcggc cggaaggagt atcctgaaat catgcgctat
     2761 ctggtgcttg ccgtgttcgt cctgatcgtc ctcagcctgg cgtcggggct gtacttcgtg
     2821 ttccacgacc gcagcgactc caagcgcgcg gtgaaggcgc tcgcggtgcg cgtcgggctg
     2881 tccgtaggac tgttcgtgtt cctgatggtt gcgtactact tcggatttgt gccggcgcgg
     2941 ctgtgaaccg tcgggggcag gcgacgcaaa cgcgtcggag cccgggtttg atcttcatag
     3001 gtctcggctt ttttggcggg gtgaatcttc ccgccaaaaa agccgagatg tattgaaggc
     3061 gaaagcccgc cgaagcgctc gccggggcgg cgtgctacag ccaataaacg aagatgaaca
     3121 accccagcca caccacgtcg acgaagtgcc agtaccaggc cacggcctcg aacgcgaagt
     3181 gatggtcggg cttgaagtgt cccttcaggc agcgcgcgaa gatcaccgac agcatgatcg
     3241 cgccgacggt gacgtggaag ccgtggaagc ccgtcagcat gaagaaggtc gagccgtagg
     3301 cgccggtggt gagcttgagg ttgaggtcgg cgtaggcgtg gccgtactca taggcctgca
     3361 cgccgaggaa gatgaagccg agcgcgatgg tcaagaacag gaacaggttc agcatcgcgc
     3421 ggttgccctc cttcagcgcc cagtgcgcga cggtcacagt gacgccggag gacagcagca
     3481 gcagcgtgtt gatcgcgggc aggccccagg cgcccatcgg cgagaaacgc tcctcgaagc
     3541 ccgggccggc cgagggccag gtgctcttga aatcgggcca cagcagctcg ttgtgctcga
     3601 ggctcgccag gtcgggcacc gacagcacgc ggatgtagaa cagcgccccg aagaacgcgg
     3661 cgaagaacat cacctcggag aagatgaacc agctcatgcc ccagcggaag gacacgtcct
     3721 cccagccgtg gtacttgccg ccctcggact cggcgatgac gtcgccgaac cagcggaaca
     3781 tcatgtagac gagcagcacg gcgccggcgg cgatcggcac ccagccgccc ttcgaggcgt
     3841 tcatcacgaa cacgccgccg agcgccatga gaaacagcga catcgagccg agcagcggcc
     3901 agtacatggg ctgcggcacg tagtagtgct gccgctgggt ggttgcgcct tgcgccatct
     3961 ttgtctcccg agtctcttgg tttcccgtat gcctcagccg gccacgatcc ggacgatcgt
     4021 gaccagcgtg aacaccaaca gcgcggccag cgccacgccc gcgacgatca cgtgcaccgg
     4081 gtcgatacgc tggctttcat ggtccgcctt gcggcggatg ccgatcattc ccgacagcac
     4141 ggtcttcacc gccgccttca cgacttgcct ccgcggcccg ccacctcgaa gaaggtgtac
     4201 gagagcgtga tggtgttgac gtcccgcggc agcttcgggt cgaccacgaa caccaccggg
     4261 aagcgccgcg tctcgccggc ggcgagcgtc tgctgcgtga agcagaagca ctcgaacttc
     4321 cggaagaact cgcccgcgcg ctgcgggccg tagctcggca ctgcctgcgc cgtgaccggc
     4381 gcgctgcgca cgttggcgat ctcatactcg acggtggcga cctcgcccgg gtggacgcgg
     4441 atgtggcgca ccagcggccg gaagctccac ggcaggtcgt gcgcgttggc gtcgagctcc
     4501 accgtcacgg tgcgcgtcgc gtcgatctgc gtgttcgccg gccgcacgtc ggccttgaga
     4561 aagtcgttca cgccgagcgc gctgcagatg gcgtcgtaga acggcaccag cgcgtagccg
     4621 aagccgaaca tggccacggc gaccaccatg agccgcagca gcatgcgccg gttgtcctcg
     4681 cgccgcgggg cctgcggctc gctcatacgt ccatgatcca gtacttgagg atcagccccg
     4741 cgaaccacgc cagcgcgatc gtggcgagga tgaggccggt cttcgcgttc ttggacagcg
     4801 ccatcatcag cgcaggtcgg gctgcggatc gaaggtgtgg tacggcgccg gcgtcggcag
     4861 gtgcgtccat tccagcgagt cggccccttc ccagggcttg tcggccgctt tcgggccgct
     4921 gcggatgcac ttcagcacga cgtacaggaa caggagctgc gagagcccga agccgaaggc
     4981 gccgatcgag ctgatcttgt tccaggtctc gaacatcagc gggtagtccg ggatgcgacg
     5041 cggcatgccg gcgaggcccg agaagtgctg cacgaagaag gtgacgttga agaagatcat
     5101 cgtcagccag aagtgccact tgccgagggt ctcgtcgtac atgcggccgg tccacttcgg
     5161 cagccagaag tacactccgc cgaacgccgc gaacagcgcg ccggccacca tcacgtagtg
     5221 gaagtgcgcc accacgtagt aggtgtcgtg cagctggatg tcgatcggca ccagcgacag
     5281 caccaggccc gtgaacccgc cgagcgtgaa caggaacagg aagccgcagg
//
LOCUS       DBNHKE010000177         9972 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_83933, whole genome shotgun sequence.
ACCESSION   DBNHKE010000177 DBNHKE010000000
VERSION     DBNHKE010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_83933"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="AC16O9_12215"
     CDS             <1..678
                     /locus_tag="AC16O9_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6517417.1"
                     /translation="VVDSSPMYGSSESVVGELCAQLGICEPLFLATKVWTQGREEGTR
                     QMQRSIERMRAAPLDLMQVHNLVDVETHTKTLLEWKSQKRVRYIGITHYATSGHAATE
                     RWLKTKQYDFVQINYSLDEPESETRILPLARELGVAVMINRPFATGSMFRRTRGKPLP
                     PWAAELGIASWAQYFLKWIVAHPAVTCAIPATSKPAHMRDNAGAGMGAMPDAAGRRRM
                     QEYYRAL"
     gene            complement(675..2117)
                     /locus_tag="AC16O9_12220"
     CDS             complement(675..2117)
                     /locus_tag="AC16O9_12220"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6517418.1"
                     /translation="MNTALWSATRLAQALRAKKLGAVELFDLYAARVRQHNRALNAIC
                     VMDLEAGRKAARAADRAKGRKGPLHGVPMTVKESFDVAGWPTTRGLTELKGNIAKRDA
                     LAVQRLKSAGAIVFGKTNVPAYTADWQTFNPLYGTTNNPWDLLRTPGGSSGGSAAVLA
                     AGLAGLELGSDIAASIRNPAHYCGVYGHKPTWGVCSTKGHSYPGALQPGDIVAIGPMA
                     RGADDLRLCMDLIAGADEIDGTGWKVSLPKPKLASLRKLRVAVMPSHSTAEVDAAVSG
                     AIERLASALAKKGARVSDKARPDFNPDEAHKVFIALLRAATSNRQAPHHVELWERLSK
                     ERPATDMSYEAQSARANVMRHKEWLAWSNRRHQMRLLWAEFFRSWDVLLCPPAATPAF
                     PQNQRGERWERMIDVNGKPQPSTTQMFWAGYSGMCLLPSTVAPIELAGGKLPVGVQIV
                     GPQYGDYLTIGVARLIEKEYFAFTAPPGYD"
     gene            complement(2114..3775)
                     /locus_tag="AC16O9_12225"
     CDS             complement(2114..3775)
                     /locus_tag="AC16O9_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6517419.1"
                     /translation="MQFDCDVLVAGSGAGGLAAAVTARKAGLKVLVAEKEAVFGGTTA
                     LSGGWLWIPNHPMQKEIGVADSMQDAATYLLHEAGEKYDAERVDAFLRAAPRMVEFFS
                     RETAVQFDASATFPDYHPDAPGGRPGGRSIVARAFDGRDLGKKLRWLRPPLPELTVFG
                     IMIGSGAELVHFMRWSKSLESALFVARRLLGHGAATLFHGRGVRLTNGNALAGRLLKS
                     ALDAGVELWSGAPVRELTREGSAVTGAVVERDGEPVRVRAQKGVVLACGGFPQDAARR
                     ARLFPHAEHHSPAPPGNTGDGLRLAQVAGARLDESLPNAAAWVPVSRVPRGGGAWGLF
                     PHFIDRAKPGVIAVTAKGVRFVNEGNSYHDFVQALLRTGETHAWLVADHRAIRAYGLG
                     YVKPRPLPLGPHLDSGYLLRGDTLGALARVAEIDAALETTVEEFNRHAKMGTDPIFRK
                     GENAYNRFYGDPDIKPNPCVAPLAQPPFYAVRVEIGDLGTYAGILTNGHAQVLDEHKR
                     PIAGLYAAGNDALSIMGGNYPGPGITLGPAMTFGWLAGRHLAGET"
     gene            complement(3894..5126)
                     /locus_tag="AC16O9_12230"
     CDS             complement(3894..5126)
                     /locus_tag="AC16O9_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HIR6517420.1"
                     /translation="MEILVIGGGIGGLTLALSLHEAGIACRVFEAAPEVKPLGVGINL
                     LPHGMRELTELGVHERIAKVAIETRAIHFYNRFGQLIFDEPRGRYAGYEWPQYSVHRG
                     DLHRELLEAVRERLGAGCVQLGSKCAGLEQDASGVTVRFEDGSSARGAAAIGCDGIHS
                     AVRRHLHPDEGPPAFQGINMWRGVTRMTPFLGGATMAVAGWLAVGKMVIYPIRDRVDA
                     QGRQLINWVAEIQSPRHVRQDWNLNGRLEDFYPIFEGWRFDWLDVARLLRESDTVLEY
                     PMVDRDPLPFWSQGRITLLGDAAHPMYPRGSNGAGQAILDARVLTGCLKREPDVTGAL
                     KAYEAARLEAAYKVVLMNRKDPPDAILREVHLRTGDKPFERIEDVVPRQELAEIMETY
                     KRVAGFSRDALARRSRLV"
     gene            complement(5136..7022)
                     /locus_tag="AC16O9_12235"
     CDS             complement(5136..7022)
                     /locus_tag="AC16O9_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR6517421.1"
                     /translation="MNQSVAGALRGALATALTLASIAWALDLPLWLRLDIYPAQFYAA
                     MLGLALPLAFLALPARRKAARGAVPWYDALAALAGLAAVAYLTWQYQALVDLILLRPR
                     GGVIAGAVAIVLALEALRRSTGWTLPVICAVFIAYALWGDALPGPLAARATDWQKLSA
                     YLALDVNGMLGLPLAVACTIVIAFLLFGELLNASRGGRFFTDIAMLSMGRFRGGPAKM
                     AVVGSALFGSVSGSAVANVVATGVITIPLIKKSGYPAHKAGAIEAVASTGGQLMPPIM
                     GASAFLMAEFLQISYAEVVLAALLPAILYYVALFVQADLDAARLGIAPLERAALPPAR
                     AVLPGLYFLLPFAVLLVALFGFNQTPQLSALAGGVLLMPLALALGYRGERPRWREFLR
                     AVPAAGYAVLDIVLVCVAAGIVIGVLGISGLGFNLTQALVQIGEGSLALLLVLTALVC
                     IVLGMGLPTVGVYVLLAALVAPALVKVGISPIAAHLYVMYFGMMSMITPPVAIASFAA
                     AGIAGADPMRTGWSAVAFGWTAFVVPFLFVLSPQLLLQGAPWAVALAAATAMAGVWLA
                     SIAVTGYFLRPLAAPWRVAFAVSGLMALIPAGAFPGAIYTDVAGMLAGLGLIGREIAL
                     RRRR"
     gene            complement(7019..7990)
                     /locus_tag="AC16O9_12240"
     CDS             complement(7019..7990)
                     /locus_tag="AC16O9_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6517422.1"
                     /translation="MDRGLIGILVPVALAGALAGGGAHAQTVGVAASNPGSLYHSTGT
                     AVAKLANDVAGLKATVYPFASATIYLPAVNAGEYAFGISNVEELRVAVLGEDQFKGRP
                     YADLRAAAILYPLRVTYFVRKDSDIRTMADLKGKRLTDGFSSQKTVPPLLDALLATGG
                     LTRADTQPVNVPNVVGGAEAFISGKADAFFFALGAAKVEEANASVGGIRALDVANTPE
                     NLARVQKFFPPAYLRAEKPSPRNVGVLEPIHALAYDGVLVASTKTPDEVVYRMVKSMH
                     GHKKVMAEVFGAMNLFDPGRMATALGPIQWHPGAVRFYTEQGAWPPK"
     gene            complement(8009..9019)
                     /locus_tag="AC16O9_12245"
     CDS             complement(8009..9019)
                     /locus_tag="AC16O9_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6517423.1"
                     /translation="MKLDIFNHIFPAAYYKRMLEVAPRGKDMHKRVREIPCIVDLDER
                     FRIMDQFGADYAQVICLPSPPIEVFGPPKLTNELARLANDGMAELVRRFPERFPAFIA
                     SLPMNDPKGLLREAKRAIRDLGAVGVQVFTNVLGVPLTARATMPLFELMAELDRPIWL
                     HPARGAETPDFRGESRSHYEIWWTFGWPYETSAAMAHIVFAGLFDRFPDLKIITHHMG
                     GMVPYFEGRVGPGWDQLGKRTSDVDYTKLLRKLRKRPIDYFRMFYADTALFGAWEATK
                     CGLKFFGADHVLFASDAPFDPEKGSMYTRTTIEIVERLDVSPEERHAIYEGNARRLMK
                     LD"
     gene            complement(9072..>9972)
                     /locus_tag="AC16O9_12250"
     CDS             complement(9072..>9972)
                     /locus_tag="AC16O9_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6517424.1"
                     /translation="LGWDPHAALKDADLVLVVDCDVPWLPRQGSPRDEATIVHIGPDP
                     LFARYPMRGFRTDIALTGRVAPVLEQLCARIATHAPSARAIGARREALAAQSAALRAR
                     GRAGGAPLSSQAVSAAINAALPEDALLVNEYPLVLEEMTIREPGRYFGTPPSGGLGWG
                     LGAALGAKLAAPGRTVVCTLGDGSYMFGNPTPGHFVSQAQGLPVLFVVFNNARWAAVH
                     RATLADYPDGYAAKARVPPFSALEPAPRFEHVVRASNGHGETVTEPAALKPALERALR
                     IVREEKRQALVDVCIAASYVKTS"
BASE COUNT         1541 a         3557 c         3478 g         1396 t
ORIGIN      
        1 gtggtcgact cctcgcccat gtacggctcg agcgagtcgg tggtcggcga gctctgcgcc
       61 cagctcggca tctgcgagcc gctgtttctc gccaccaagg tctggacgca ggggcgcgag
      121 gagggcacgc ggcagatgca gcgctcgatc gagcgcatgc gcgccgcgcc gctggacctg
      181 atgcaggtgc acaacctcgt cgacgtcgag acgcacacga agacgctgct cgaatggaaa
      241 tcgcagaagc gcgtgcgcta catcggcatc acgcactatg cgacctcggg ccatgccgcg
      301 accgagcgct ggctgaagac gaagcagtac gacttcgtgc agatcaacta ctcgctcgac
      361 gagccggagt cggagacgcg gatattgccg ctggcgcgcg agctcggcgt cgccgtgatg
      421 atcaaccggc cgttcgcgac gggcagcatg ttccggcgca cgcgggggaa acccctgccg
      481 ccgtgggcgg cggagctcgg catcgcgagc tgggcgcagt acttcctcaa gtggatcgtg
      541 gcgcatccgg ccgtgacctg cgccattccc gcgacctcca agcccgcgca catgcgcgac
      601 aacgcgggcg cggggatggg cgcgatgccg gacgcggcag gccggcgccg catgcaggag
      661 tactaccgcg ccctttagtc gtatcccggc ggggccgtga aggcgaagta ttccttctcg
      721 atcagccgcg ccaccccgat cgtcagatag tcgccgtact gcgggccgac gatctgcacg
      781 ccgaccggga gcttgccgcc ggcgagctcg atcggcgcca ccgtcgaggg cagcaggcac
      841 atccccgagt acccggccca gaacatctgc gtggtcgagg gctgcggctt gccattcacg
      901 tcgatcatgc gctcccagcg ctcgccgcgc tggttctgcg ggaacgcggg cgtcgccgcc
      961 ggcgggcaga gcagcacgtc ccaggacctg aagaactcgg cccacagcag ccgcatctgg
     1021 tggcggcggt tcgaccaggc gagccactcc ttgtgccgca tgacgttggc gcgcgcggat
     1081 tgcgcttcgt agctcatgtc ggtggccggg cgttccttcg acagccgctc ccacagctcg
     1141 acgtgatgcg gggcctggcg gttcgaggtc gcggcgcgca gcagcgcgat gaacaccttg
     1201 tgcgcctcgt ccgggttgaa gtcgggccgc gccttgtcgg acacccgggc gcccttcttt
     1261 gccagcgcgc tcgccaggcg ctcgatcgcg cccgagaccg ccgcatccac ctccgccgtc
     1321 gaatgcgacg gcatcaccgc cacgcgcagc ttgcgtagcg acgcgagctt cggcttcggc
     1381 agcgagacct tccaaccggt gccgtcgatc tcgtctgcac cggcaatgag gtccatgcac
     1441 aggcgcaggt catcggcgcc gcgcgccatc ggcccgatgg cgacgatgtc gcccggttgc
     1501 agcgcgcccg ggtacgagtg tcctttcgtc gagcacacgc cccaggtcgg cttgtgtccg
     1561 tacacgccgc agtaatgcgc cggattgcgg atcgaggcgg cgatgtcgct gcccagctcc
     1621 agccctgcga gtccggccgc gagcacggcc gccgagccgc cgctagagcc gccgggggtg
     1681 cgcagcaggt cccaggggtt gttggtggtg ccgtagaggg gattgaaggt ctgccagtcg
     1741 gcggtgtagg ccggcacgtt ggtcttgccg aaaacgatgg cgccggcgga cttgagccgc
     1801 tgcacggcga gcgcatcgcg ctttgcgatg ttgcccttca gctccgtaag gccgcgcgtc
     1861 gtgggccagc cggcgacgtc gaaggactcc ttcaccgtca tcggcacccc gtgcagcggg
     1921 ccctttcttc ccttcgcgcg gtcggcggcg cgcgcggctt tgcggcccgc ctcgagatcc
     1981 atcacgcaga ttgcgttcag cgcgcggttg tgctggcgca cgcgcgcggc gtagaggtcg
     2041 aacagctcca ccgcgcccaa cttcttcgcg cgcagcgcct gcgcgagccg ggtggcggac
     2101 cacaaggccg tgttcatgtt tcccccgcaa gatgccggcc ggcgagccag ccgaaagtca
     2161 tggcgggccc gagcgtgatg ccgggcccgg gatagttgcc gcccatgatc gagagcgcgt
     2221 cgttgccggc cgcatacagg ccggcgatcg ggcgcttgtg ctcgtccagg acctgcgcgt
     2281 ggccgttggt gaggatgccg gcgtaggtgc cgaggtcgcc gatctcgacg cgcaccgcgt
     2341 agaacggcgg ctgcgcgagc ggcgccacgc aagggttcgg cttgatgtcc ggatcgccgt
     2401 agaaccggtt gtaggcattc tcgcctttgc gaaaaatggg gtcggtcccc attttcgcgt
     2461 gccggttgaa ttcttccacg gtcgtctcga gcgccgcgtc gatctcggcg acgcgcgcga
     2521 gcgcaccgag ggtgtcgccg cgcagcaggt agcccgagtc caggtgcggg ccgagcggca
     2581 gcggacgcgg cttcacgtat cccaggccgt aggcgcggat cgcgcgatgg tcggcgacca
     2641 gccacgcatg ggtttcgccc gtcctcagca gcgcctgcac gaagtcgtgg tacgagttgc
     2701 cttcgttcac gaagcgcacg cccttcgccg tcaccgcgat cacgccgggc ttggcgcggt
     2761 cgatgaaatg cgggaacagg ccccaggcgc cgccgccgcg cggcacgcgc gacaccggca
     2821 cccaggccgc ggcgttgggc agcgactcgt cgagccgcgc gcccgcgacc tgggcgaggc
     2881 gcagcccgtc tccggtgttg ccgggcggtg cgggcgagtg gtgctcggcg tgcggaaaca
     2941 ggcgcgcgcg gcgcgccgcg tcctgcggga atccaccgca ggcgagcacc acgccctttt
     3001 gcgcgcgcac ccgcaccggc tcgccgtcgc gttcgaccac cgcgccggtg acggcgctcc
     3061 cctcgcgcgt cagctctctc accggcgcgc cactccacag ctcgacaccc gcatcgagtg
     3121 ccgacttcag cagccggccg gcgagcgcgt tgccgttcgt gaggcgcacc ccgcggccgt
     3181 gaaacagcgt ggccgcgccg tgcccgagga ggcgccgcgc gacgaacagc gccgactcga
     3241 gcgacttcga ccagcgcatg aagtgcacca gctccgcgcc cgagccgatc atgatgccga
     3301 acaccgtgag ctcggggagc ggcggccgca gccatctgag cttcttgccc agatcgcgcc
     3361 cgtcgaaggc gcgcgccacg atcgagcgcc cgcccggccg cccgcccggc gcgtcggggt
     3421 ggtagtcggg gaaggtcgcc gaggcgtcga actgcaccgc ggtctcgcgc gaaaagaact
     3481 ccaccatgcg cggcgcagcg cgcaggaacg catccacgcg ctcggcgtcg tacttctcgc
     3541 cggcctcgtg caggagatag gtggccgcgt cctgcatcga gtcggcgacg ccgatctcct
     3601 tctgcatcgg gtgattcggg atccacagcc acccgcccga gagcgcggtg gtgccgccga
     3661 acacggcctc cttctcggcg acgagcacct tgagcccggc tttgcgcgcg gtgaccgcgg
     3721 cggcgagccc gcccgcgccc gagcccgcga ccagcacgtc gcagtcgaat tgcatggcgc
     3781 atcttaaccg ggacgccacg cgtggactgg ataccgcccg aggcctcgca cacgacctgg
     3841 ggaaggcgca ggctgcctgc ggctacgtgc tgcagctctg ggtgttgcga cgctcagacc
     3901 agccggctgc gccgggccag cgcgtcacgg gagaagcccg cgacgcgctt gtaggtctcc
     3961 atgatctcgg cgagctcctg gcgcgggacg acgtcctcga tgcgctcgaa cggcttgtcg
     4021 ccggtgcgca ggtgcacttc gcgcaggatc gcatccggcg gatccttccg gttcatcagt
     4081 acgaccttgt acgccgcctc caggcgcgcc gcctcgtagg ctttgagcgc gccggtgacg
     4141 tcgggctcgc gcttgaggca gccggtgagc acgcgcgcat cgaggatcgc ctggcccgcg
     4201 ccgttcgagc cgcgcgggta catcgggtgc gccgcgtcgc caagcagcgt gatccgtcct
     4261 tgcgaccaga agggcagcgg atcgcggtcg accatcgggt actcgagcac cgtatcgctc
     4321 tcgcgcagga gccgcgcgac gtccagccag tcgaaacgcc agccctcgaa aatcggatag
     4381 aaatcctcca gccgcccgtt caggttccag tcctggcgca cgtggcgcgg cgactggatc
     4441 tcggccaccc agttgatgag ctgccggccc tgggcgtcga cgcgatcccg gatcgggtag
     4501 atcaccatct tgccgacggc cagccagccg gcgaccgcca tggtggcgcc gccgaggaac
     4561 ggcgtcatgc gcgtcacgcc gcgccacatg ttgatgccct ggaacgcggg tggcccctcg
     4621 tccgggtgca ggtgcctgcg caccgccgag tggatgccgt cgcagccgat cgccgccgcg
     4681 ccgcgcgcgc tcgagccgtc ctcgaagcgc accgttaccc cggaggcgtc ctgctcgagg
     4741 ccggcgcact tgctgccgag ctgcacgcac ccggcgccga gccgctcgcg caccgcctcg
     4801 agcagctccc ggtgcaggtc gccgcggtgc accgagtact gcggccactc gtacccggcg
     4861 taacgccccc gcggctcatc gaagatcagc tggccgaaac ggttgtagaa atgtatcgcg
     4921 cgcgtctcga tcgccacctt ggcgatgcgc tcgtgcacgc cgagctccgt cagctcgcgc
     4981 atgccgtgcg gcagcaggtt gatgcccacg ccgagcggct tcacctccgg ggcggcctcg
     5041 aacacgcgac acgcgatgcc cgcctcgtgc agcgacagcg cgagcgtcag cccgccgatg
     5101 ccgccgccga tgacgaggat ttccacgcga cgattctatc gccggcgccg caacgcgatc
     5161 tcgcggccga tcagcccgag cccggcgagc atcccggcca cgtcggtgta gatcgcgccg
     5221 gggaacgcgc ccgccgggat gagcgccatc agccccgaaa ccgcgaaggc gacgcgccag
     5281 ggcgcggcga gcgggcgcag gaagtagccg gtgaccgcga tcgacgcgag ccacacgccg
     5341 gccatcgcag tcgccgccgc gagcgccact gcccagggcg ccccctgcag caggagctgc
     5401 ggcgagagca cgaacaggaa cggcaccacg aatgccgtcc agccgaacgc gaccgccgac
     5461 cagccggtgc gcatcgggtc cgcgcccgcg atgcccgcgg cggcaaagga cgcgatcgcc
     5521 accggcggcg tgatcatcga catcatgccg aagtacatca cgtacaggtg cgccgcgatc
     5581 ggcgagatgc cgactttgac gagcgccggc gcgacgagcg cggcgagcag cacgtacacg
     5641 ccgaccgtcg gcaggcccat gccgagcacg atgcacacga gcgccgtcag gacgagcagc
     5701 agcgcgaggc tcccctcgcc gatctgcacc agcgcctgcg tcaggttgaa gcccaggccg
     5761 ctgatgccga gcacgccgat cacgatgccc gcggccacgc acaccagcac gatgtcgagc
     5821 accgcgtatc ccgccgccgg caccgcgcgc aggaactcgc gccagcgcgg ccgctcgccg
     5881 cgatagccca gggcgagcgc gagcggcatg agcagcactc cgccggcgag cgcggagagc
     5941 tgcggcgtct ggttgaagcc gaacagggcg acgagcagca cggcgaacgg cagcaggaag
     6001 tacaggcccg gcagcaccgc gcgcgcgggc ggcagcgcgg cgcgctcgag cggcgcgatg
     6061 ccgagccgcg cggcgtcgag gtcagcctga acgaacagcg ccacgtagta caggatcgcg
     6121 ggcagcagcg ccgcgagcac cacctcggcg taggagatct gcaggaactc ggccatcagg
     6181 aatgccgacg cgcccatgat cggcggcatg agctgcccgc cggtggaggc caccgcctcg
     6241 atggcgcccg ccttgtgcgc cgggtagccg cttttcttga tcagcgggat ggtgatcacg
     6301 ccggtggcga cgacgttggc gaccgcgctc cccgacaccg agccgaacag cgccgagcca
     6361 accaccgcca tcttcgccgg gccgccgcgg aagcgcccca tgctgagcat ggcgatgtcg
     6421 gtgaagaagc gcccgccgcg gctcgcgttg agcagctcgc cgaacagcag gaacgcgatc
     6481 acgatggtgc aggccaccgc gagcggcagc ccgagcatgc cgttcacgtc gagcgcgagg
     6541 taggccgaga gcttctgcca gtcggtggcc cgcgccgcga gcggacccgg cagcgcgtcg
     6601 ccccacagcg cgtaggcgat gaacacggcg cagatcaccg gcagcgtcca gccggtcgag
     6661 cggcgcagcg cctccagcgc cagcacgatg gcgacggcgc ccgcgatcac gccgccccgg
     6721 ggccgcagca ggatcaggtc gacgagcgcc tggtactgcc aggtcaggta ggcgactgcg
     6781 gccaagcccg ccagcgcggc gagcgcgtcg taccagggca cggcgccgcg cgccgccttg
     6841 cggcgcgcgg gcagcgcgag gaacgcgagc ggcagcgcga gccccagcat cgcggcgtag
     6901 aactgcgccg gatagatgtc caggcgcagc caaagcggca ggtcgagcgc ccacgcgatc
     6961 gaggccagcg tgagcgccgt ggcgagcgcg ccgcgcagcg cgcccgcgac agactggttc
     7021 acttcggcgg ccaggcccct tgctcggtgt agaagcgcac ggcgcccgga tgccactgga
     7081 tcgggcccag cgccgtcgcc atgcgcccgg ggtcgaacag gttcatcgcg ccgaacacct
     7141 cggccatcac cttcttgtgc ccgtgcatgg acttcaccat gcggtagacg acttcgtccg
     7201 gggtcttggt gctcgccacc agcacgccgt cataggcgag cgcgtggatc ggctcgagca
     7261 cgccgacgtt gcgcgggctc ggcttttccg cccgcagata ggcgggggga aagaacttct
     7321 gcaccctggc gaggttctcc ggcgtgttcg ccacgtcgag cgcgcggatg ccgccgaccg
     7381 aggcgttggc ctcctccacc ttggccgcgc ccagcgcaaa gaagaaggcg tcggccttgc
     7441 ccgagatgaa ggcctcggcg ccgcccacca cgttcggcac gttgaccggc tgggtgtcgg
     7501 cgcgcgtcag gccgccggtg gcgagcagcg cgtcgagcag cggtggcacg gtcttctggc
     7561 tgctgaagcc gtcggtgagg cgcttgccct tcaggtccgc catcgtgcga atgtcggagt
     7621 ccttgcgcac gaagtacgtg acgcgcagcg ggtacaggat agccgccgcg cgcaggtccg
     7681 cataaggccg ccccttgaac tggtcttccc cgagcacggc gacgcgcagc tcctcgacgt
     7741 tcgagatgcc gaacgcatac tcgcccgcgt tcaccgcggg gaggtagatc gtcgcgctgg
     7801 cgaacggata gaccgtcgcc ttcagcccgg ccacgtcgtt ggcgagcttc gccaccgcgg
     7861 tgccggtcga gtggtagagc gagcccgggt tggacgccgc gacgccgacg gtctgcgcgt
     7921 gcgcgccgcc tcccgcaagc gcgccggcga gcgccaccgg cacgagtatc ccgatcaatc
     7981 ccctgtccat tgtcatcctc ctttgttgtc agtccagctt catcagccga cgcgcgttgc
     8041 cctcgtagat cgcgtggcgc tcctcgggcg acacgtcgag ccgctccaca atctcgatcg
     8101 tggtgcgcgt gtacatcgac cccttctccg ggtcgaaggg cgcatccgag gcgaacagta
     8161 catgatcggc gccgaagaac ttcagtccgc acttggtcgc ctcccacgcg ccgaacagcg
     8221 ccgtgtcggc gtagaacatg cggaagtagt cgatggggcg cttcctcaac ttgcgcagga
     8281 gcttcgtgta atcgacgtcc gaggtgcgct tgccgagctg gtcccatccc ggcccgacgc
     8341 ggccctcgaa gtacggcacc atgccgccca tgtggtgggt gatgatcttc aggtccggga
     8401 agcggtcgaa caggcccgcg aagacgatgt gcgccatcgc cgcgctggtc tcgtagggcc
     8461 agccgaacgt ccaccagatc tcgtagtggc tgcgggactc gccgcggaag tccggggtct
     8521 ccgccccgcg cgccgggtgc agccagatcg ggcggtcgag ctcggccatc agctcgaaca
     8581 gcggcatcgt cgcgcgcgcg gtgagcggca cgccgagcac attggtgaac acctgcacgc
     8641 ccacggcgcc caggtcgcgg atcgcccgct tcgcctcgcg cagcagtccc ttcgggtcgt
     8701 tcatcggcag cgaagcgatg aacgcgggaa agcgctccgg gaagcggcgc acgagctctg
     8761 ccatgccgtc gttggccagc cgggcgagct cgttggtgag cttcggcgga ccgaagactt
     8821 cgatcggcgg cgagggcagg cagatgacct gcgcgtagtc cgcgccgaac tgatccatga
     8881 tgcggaagcg ctcgtcgaga tcgacgatgc acgggatctc gcgcacccgc ttgtgcatgt
     8941 ccttgccccg cggggcgact tcgagcatgc gcttgtagta cgccgcggga aagatgtgat
     9001 tgaagatgtc gagcttcatg ggcgaggact gtaccggccg gccagagcgc cgacaagcac
     9061 ggccttcgag ctcagctggt cttcacgtag cttgctgcaa tgcaaacatc gaccagcgcc
     9121 tggcgtttct cctcgcgcac gatacgcagc gcgcgctcga gtgccggctt gagcgccgcc
     9181 ggctcggtca cggtctcgcc gtggccgttc gaggcgcgaa cgacgtgctc gaagcgcggc
     9241 gcgggctcga gcgccgagaa cggcggcacg cgcgccttgg ctgcgtagcc gtcgggatag
     9301 tcggcgagcg tcgcgcggtg caccgccgcc cagcgcgcat tgttgaacac cacgaacagc
     9361 accggcaatc cctgcgcttg cgaaacgaag tgcccgggcg tcgggttgcc gaacatgtac
     9421 gagccgtcgc cgagcgtgca gaccaccgtt cgcccgggcg cggcgagctt cgcgccgagc
     9481 gcggcaccca ggccccagcc caggcccccg gaaggcggcg tgccgaagta gcgccccggc
     9541 tcgcggatcg tcatctcctc gagcaccagc ggatattcgt tcacgagcag cgcgtcctcg
     9601 ggcagcgctg cattgatcgc ggccgacacg gcctgcgagg aaagcggcgc cccgccggcg
     9661 cggccgcgcg cgcgcagcgc cgcgctctgc gccgcaagcg cctcgcgccg ggcgccgatc
     9721 gcgcgcgcgg agggagcgtg cgtcgcgatg cgcgcgcaca gctgctccag cacgggcgcg
     9781 acgcgtccgg tgagcgcgat gtcggtccga aagccacgca tcggatagcg cgcgaagagc
     9841 ggatcggggc ctatgtgcac gatcgttgcc tcgtcccgcg gactgccctg acgcggcagc
     9901 cagggcacgt cgcagtcgac caccagcacc agatcggcat ccttcagcgc cgcgtgcggg
     9961 tcccagccga gg
//
LOCUS       DBNHKE010000178        31197 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_136776, whole genome shotgun sequence.
ACCESSION   DBNHKE010000178 DBNHKE010000000
VERSION     DBNHKE010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 31197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31197
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_136776"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="AC16O9_12255"
     CDS             complement(<1..1535)
                     /locus_tag="AC16O9_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6517425.1"
                     /translation="MKVTVIGGGPGGLYFALLAKKAWPRWDITCYERNRPDDTFGFGV
                     VFSDQTLDTFKAYDAASYERIRRRFAYWGDVDVVYKGRTLRSGGNGFCGCSRNALLAI
                     LQDRCRELGVAIRFQTEVEGLEQFADSDLVVVADGINSKIREQHKAHFKPSVDLRPNK
                     FTWLGSTRPLDAFKYFFRETPEGIILAHCYQYEEGRSTWVIETDEATWKNFGFDKMGE
                     AEMLPVLERVFAEELDGHPLLANRSIWRNFPTIVNQTWVKGNAVLVGDAKATAHFSIG
                     SGTKLAMEDAIALYEALRTEAGVKPALARYDTARREEVEKTQHAANVSLAWFEHMRRY
                     WGMAPEQFAFGVMSRSKQITWENLELRDPAYVQAMHRWFAAQVKAQGFDIDMKNPPVP
                     MFTPLRLRDMVVENRVVVSPMDQYSAVDGVPTDWHFVHLASRAVGGAGLVYVEMTCPS
                     PEARISPGCTGLWNEAQRDAFKRIADFCHANSKAKLCMQIGHSGRKGSTQLGWERMDH
                     PLE"
     gene            complement(1537..1938)
                     /locus_tag="AC16O9_12260"
     CDS             complement(1537..1938)
                     /locus_tag="AC16O9_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6517426.1"
                     /translation="MMTILQPPGWAKPRGYANGIAVKGGTTVYIAGQVGFTGQGEWRE
                     KGFAGQFRQALQNILDVLAEARGRPEHVVRLTWYVLDKAEYLASLKDVGAAYRELMGR
                     HYPVMAVVQVSGLVEDAARLEIEATAVVPGD"
     gene            complement(1935..3551)
                     /locus_tag="AC16O9_12265"
     CDS             complement(1935..3551)
                     /locus_tag="AC16O9_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIR6517427.1"
                     /translation="MKVKSAHLDTFARDNLPPRSQWPEFLLERPEFRYPVRMNCATEL
                     LDKMVSRGFGHRPVIRASDGSCTYTELYAQANRIANVLVRDMGLKPGNRVLLRGPNSP
                     MMAACWFAVMKAGGVCVATMPLLRAKELTDIVRKAEIGHALCDKRLAAELEAARAGCP
                     TLKAVKYWHDDARDSLDALCVRSPQVFANVDTAAEDVALIAFTSGTTGKPKGTLHFHR
                     DVIAMCDAFPRSCLGATKDDVFCGTPPLAFTFGLGGLLAFPMRVGACTVLMEKATPET
                     LLETLQREKATVCFTAPIMYRAMAGLAKDYDLGALKKCVSAGEALPDATRQLFKQATG
                     IEIIDGIGSTEMIHIFISHTPERVRRGATGYAIPGYRATVLDEAGRPCRPGVIGRLAV
                     KGPTGCRYLADERQTDYVQNGWNVTGDAYLMDEDGYFFYQARTDDMIISAGYNIAGPE
                     VEGALLAHPAVAECGVVGAPDAERGAIVKAYVVLKPEARGSEALAKELQEHVKNAIAP
                     YKYPRAIEFVDALPRTETGKLQRFKLRTPT"
     gene            complement(3548..4651)
                     /locus_tag="AC16O9_12270"
     CDS             complement(3548..4651)
                     /locus_tag="AC16O9_12270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517428.1"
                     /translation="MDRFAHLDWPFFDPPHAALSRAVDTWALAHSAHAHGEDADAICR
                     RLVKDLGQAGYLKYCVNEQPDVRSIALIREVLAYHAGLADFSFVMQGLGSGPIVLAGS
                     EKQRRDYLPRAAAGEAIAAFALSEPDAGSDVQAMTTTAKKQGADWVLDGVKTWISNGG
                     IADFYVAFAKTDKGISAFVVEAKDADASERIDIVAPHPMATVKFKGARGTLIGDEGQG
                     FKLAMKNLDVFRTTVAAAALGFARRGLDEALARARERRMFGQRLADLQMTQAKLADMA
                     TAIEAAALLTYRSAWLRDVKRARVTREAAMAKMQATEAAQQVIDDAVQILGGLGVMRG
                     HPVERLYREVRALRIYEGATEVQKVIIARELLK"
     gene            complement(4651..5115)
                     /locus_tag="AC16O9_12275"
     CDS             complement(4651..5115)
                     /locus_tag="AC16O9_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6517429.1"
                     /translation="MDRETRATVDDHRALRLWLRLLTCSQLIERHVRSRLRERFGTTL
                     PRFDLLSQLERHPRGLKMSELSRLLMVTGGNVTGIVDQLVKEGLVERVDDAEDRRAWR
                     VTLTRAGDKAFAEMARAHEEWVIELLGGLTRKESEALMQLLARLKQTTLEHS"
     gene            complement(5176..5937)
                     /locus_tag="AC16O9_12280"
     CDS             complement(5176..5937)
                     /locus_tag="AC16O9_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6517430.1"
                     /translation="MEAAFQLADVGAAELALIAAVALAGSILGGLAGYGTGLVVPIFL
                     APVVGVVNVVPVMAVGMIFANGSRVAAFWRDIHKPHARRILIMGLPACVAGAYAYTLL
                     EARWVALLLGSFFIVSVPIRRLLARAGWRLEARGVTIAGAGFGFVNGSMTGVGLLLIS
                     MLMAAGLEGASLIATDAIVSVVMGVAKIVLFGSVARLDAELALAGVLIGLCTMPGAFI
                     ARRLLALVPLKVHTAFMDVVVLGGGASFLWRAWHG"
     gene            complement(5886..6992)
                     /locus_tag="AC16O9_12285"
     CDS             complement(5886..6992)
                     /locus_tag="AC16O9_12285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HIR6517431.1"
                     /translation="MSTHEHRRAATRAGLTYVSDGLAGITRRRVGTGWAYYTPDGQRI
                     ADAATRKRLNALAIPPAWTEVWICPDPGGHIQATARDARGRKQYRYHPSYREARDQSK
                     FRRMLEFSEALPHLRERVERDLRGDDLGRAQLLATVVRLLDRTLIRVGNDEYARENRS
                     FGLTTLRRRHVQVDGNQLRFTFRGKSGVEHSISIAEPRLARIIQRCQDLPGQEMFQYL
                     NAMGKREAVTSDDVNEYLREASGRDVTAKDFRTWGGTMLAAVELRAMGPAASRREADR
                     NILRAIDAVAERLGNTRAVCRKYYVHPALLHAYLLGRTVPQPSAAPPRAKRAGRAGGA
                     LRRDEVAVLQFLQESIPEWKPRSSSPTSAPPSSP"
     gene            complement(6989..7876)
                     /locus_tag="AC16O9_12290"
     CDS             complement(6989..7876)
                     /locus_tag="AC16O9_12290"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6517432.1"
                     /translation="MAAGFHGVFPYVVSPVLASGEVDATVLARLCDDLIAAGVHGLTP
                     LGSTGEFAYLNWTQRRRVVEVVIEAARGRVPVVAGVASTTVADAVFQAREMERIGCNG
                     ILAILEAYFPVGDEGVYAYFRAIAEAVRIPVVLYTNPNFQRSDLSLPVIERLSRLPNI
                     GYIKDASVNTGRLLSILNRVEGRMQVFAASSHVPACVMLLGGVGWMAGPACVAPRQSV
                     ELYELCRRGEWTAAMEKQRPLWALNQAFAKYNLAACIKGALELQGYAVGAPLAPQAPL
                     APEGIEEVRRALSAIGALP"
     gene            complement(7880..9583)
                     /locus_tag="AC16O9_12295"
     CDS             complement(7880..9583)
                     /locus_tag="AC16O9_12295"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HIR6517433.1"
                     /translation="MSANKPQGLARGLTNYGDAGFSLYLRRSFAKSMGYSGEMLERPV
                     VGIADSKSGFNNCHRHFPELIEAVKRGVLAAGGLPIEFPTVSLGEVFLSPTSMMFRNL
                     MSIDVEEMIRAQPMDAVVLVGGCDKTVPAQLMGAASADVPAVQLLAGPMMPTSFHGER
                     LGACTDCRRFWALHRAGKVDAAGIGEVEGNLATTAGTCAVMGTASTMASIAEALGMAL
                     PGTAAIPAVHADRLRAGEASGRRAVELVKAPIRPSQVITEKSVENALRVLLAIGGSTN
                     AIVHLTAIAGRAGVRVDLKRLNELSDSTPVLVDLKPTGQHYMSDLYAAGGVGAVLREL
                     KPLLNLECMTVAGETLGERLEQEKGAWVDRAVVRPLAEPLAKQGGLVALFGTLAPNGA
                     ILKRSAADPGLFEKEGRAVVFSSLDDLAARVDDPALDVTADDFMVLQNAGPKSGYAMP
                     EAGYLPIPTKLARAGVKDMVRISDARMSGTAYGTIVLHVSPEAAAGGPLALVKHGDRI
                     RISVKARRIDLAVPEAELAKRKAAWQPPAAMPARGYARLYMDHVLQAEHGCDFDFLRK
                     A"
     gene            complement(9591..10253)
                     /locus_tag="AC16O9_12300"
     CDS             complement(9591..10253)
                     /locus_tag="AC16O9_12300"
                     /inference="COORDINATES: protein motif:HMM:NF053611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaD family glycine zipper/OmpA-like
                     lipoprotein"
                     /protein_id="HIR6517434.1"
                     /translation="MKKSWIAMIVGGALLAGCQTDPNRPMTRTQSGAMIGALGGAVVG
                     AMAYKKNRTKGAVVGAIGGGLAGAAVGNYMDGQKRDLEKNLAPEIQAGQVRVQKLSEQ
                     VVLVTMTSQTAFEVDSTNVNPGFRSTLDKLADVVVRYGKTTLTVVGHTDSTGSNTYNQ
                     KLSEERALSVAQYLESRNVNGMRLATAGKGETVPVASNASEAGRQANRRVEIYVEAVV
                     QG"
     gene            complement(10325..11059)
                     /locus_tag="AC16O9_12305"
     CDS             complement(10325..11059)
                     /locus_tag="AC16O9_12305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6517435.1"
                     /translation="MAKVEPMDPHPASVAETDARRRFTRRMFDSSAPDYDRVDRLLAL
                     GSGSWYRRQALLRAGLAPGMRMLDVAVGTGLVAREALGVVGARGYVIGVDPSAGMLAQ
                     SEAHGLTLARARAEALPFAAQSFDFLCLGYALRHLADLGAVFAEFRRVLKPGGQLLML
                     EITRPAGRLAAAALKAYLRGVVPALARVLAASRDTPALYRYYWDTIEACVPPARIMAS
                     LSAAGFTRVAHGVQLGIFSEYSARLA"
     gene            11104..11436
                     /locus_tag="AC16O9_12310"
     CDS             11104..11436
                     /locus_tag="AC16O9_12310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6517436.1"
                     /translation="MAAPKSPPTTIGAVKDIVPKLAELTEKVVFGDVWARPGLSKRDR
                     SFATVAALIALHRPDQLKGHIWRALDNGVTREELAELFTHLAFYAGWPNGISAAQVAK
                     QVYEERGA"
     gene            11373..12335
                     /locus_tag="AC16O9_12315"
     CDS             11373..12335
                     /locus_tag="AC16O9_12315"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517437.1"
                     /translation="MAERHQRGAGGQAGLRGARRMKAGFVGLGVMGAHMAENLARAGH
                     AVIGTDLRKVEVAGVRWAASAAEAAAEADVVFTSLPGPKEVEALAPKLGDALPKGAAW
                     FDLTTNSPACVRRVHAQLKTLGVDFLDAPVSGGKAGAQSGRLAIWVGGDEAVYRRFEP
                     MLRAIGDQPFYVGPIGAGSIAKLAHNCASFTVQTALAEIFTLGVKAGVEPLALFQALR
                     HGATGRKRTFDRLAEQYLPGAYDPPTFAARLAHKDLSLALELAREVGVPMQVAERAFA
                     DFSEALKRGWGERDSRTPMLLQNERAGVDPHVPAAKLREVLEKG"
     gene            complement(12332..13114)
                     /locus_tag="AC16O9_12320"
     CDS             complement(12332..13114)
                     /locus_tag="AC16O9_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517438.1"
                     /translation="MRAGTAAVAGLAAVLWLGSAAAQRDPLGTRGGWEVGLLGSTYEY
                     EEPNFALLEGERVGATGSVTFLGQDYIHHHIEGRYSYAELDYTGSGTLRDVEDYILEL
                     RALVGRDYRAGRFVWVPYAGLGYRYLYNDLRGVSSTGALGYRRKSRYYYIPVGVALRF
                     PMNAGWVLAPRIEYDAFVHGKQRSYLSDTGLGFNAVTNHQRRGRGARGELALESPRWS
                     VSLWSHYWKIEDSDIQPIGMGFVGLEPANTTRETGIELRYRF"
     gene            13146..14894
                     /locus_tag="AC16O9_12325"
     CDS             13146..14894
                     /locus_tag="AC16O9_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HIR6517439.1"
                     /translation="MRLHSRLRFAPAEVRVDKRADGSTLLRSPQALRAHERCVGEWLV
                     KWARQSPERPFLAERAGEGWRKVSYAQALDAARRIGQALLERGLGPDKPLAILSDNSV
                     DHGLLALGAMHAGVPVAPVSPAYSLMSKDFAKLKAIFELVKPGLVWVDDPKKYAAALA
                     AAGAEPTPLAALLERAPGARIDEAFATLGPDTVAKILFTSGSTGLPKGVINTQRMLAA
                     NQQMIAQVWPFLEDRVQTICDWLPWNHTFGGNHNFNLVLRNGGTLYVDGGKPAPGLVE
                     VTARNLREVAGTMYFNVPRGFDLLLPFLEKDAELRRNFFRDLDVLFYAAAALPQNLWE
                     RLERLALEEKGGELAMLSAWGSTETAPSAAAVHYHIERAGVIGLPNPGCELKLVPAAG
                     KLEVRVRGPHVTPGYFRRDDLTRAAFDEEGFYRIGDAVKFADPQAPQKGLVFDGRVAE
                     DFKLTSGTWVHAGAVRVKLIAAGNPYIQDAVITGHDRDEVGALVFPSPAAKDLAPEAL
                     RAKISAALKALGAEGGTSMHPTRALVTADPPSIDANEITDKGYINQRAVLERRAALVE
                     KLHAKPPGAEVITPAR"
     gene            15131..17212
                     /locus_tag="AC16O9_12330"
     CDS             15131..17212
                     /locus_tag="AC16O9_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6517440.1"
                     /translation="MERSFREEVAKLRLGDGETFHGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHMMDVLNDARDILDELGVHVETNASEAGAAAMLGASINYPMRGAVTFKSTVGT
                     NVASDALSNLASAGVKGGALIVLGEDYGEGASIIQERSHAFAMKSQIWLLDPRPSLPR
                     IVEMVEHGFELSEASSTPVMLELRIRACHVQGSFAARRNRAPRISRRALLENPDFDFA
                     RISLPPSTYAQEKHKIDVRWPAAVRYIREQGLNEAFPGERADIGILCQGGMYNAVIRA
                     LQQLGLADAFGAARIPIYCMNVTYPLVPEEIVGFCAGKNAVLVVEEGQPAYLEDAILA
                     TLRRHDVGAVKVHGKDVLPMAGEYTGEVVVTGLSKFLGAEEVLARITAPKLKAAALLG
                     APLPTRPPGFCVGCPERPVFAAMKLVEKELGKYHVSADIGCHTFSTLPPFNIGHTVLG
                     YGLGLASNSAVQPMFGRRTVTIMGDGGFWHNGLTSGVANAVFNKDDGILVIMKNGYSS
                     ATGTQELPSSPRHSEHKAPDMSIERALQGLGVGWMRTVHTYHVGEMKKTLKDAMTTGY
                     AGLKVVIADGECQLERQRRVRPVTAKQLAAGERVVRVKYGVDEDTCTGDHSCIRLSGC
                     PTLTVKDNPDPLRQDPVATVIEGCVGCGLCGENAHAATLCPSFYRAEVIQNPSLVDRT
                     LGRLRRLVVPA"
     gene            17209..18666
                     /locus_tag="AC16O9_12335"
     CDS             17209..18666
                     /locus_tag="AC16O9_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HIR6517441.1"
                     /translation="MSLPERSVTVLIAALGGEGGGVMADWLMHAATASGFPAQATSIP
                     GVAQRTGATTYYLEIFPAQRAQLGGREPVMSLTPSPGNVDLMVASELLEAGRAMQNGF
                     VAPQRTTLIASTHRIYATVEKMQMADGRFPEERVLSAARQLAKHAVLFDMRRMAEENG
                     TVINAVLFGAMAGSGALPLPREACETAILRAGKGAQASLRGFAAGYEVAAGARPAPPA
                     AARPVPPETRQILELGAARLRDYQGEAYARLYLERIAALGNADAKLVGETGRQLALWM
                     SYEDIIRVADLKTRASRFARVRREVGARDAEPVVVTDYLKPGVEEVASLLPPALGRRV
                     RAWAERRGKLDAYNVGMHIRTSSIGGYLLVRSLAGLKPWRPRSYRYGEEQALIERWLA
                     AVRAAAPHSSALALEIVACAGLLKGYGETHRRGRASFLAILDALVENPAAGDAAARVA
                     AIRKAREAALADPEQKALAKALGRPVTWLHAAKTR"
     gene            18679..19527
                     /locus_tag="AC16O9_12340"
     CDS             18679..19527
                     /locus_tag="AC16O9_12340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6517442.1"
                     /translation="MTDPAFLSREYNNRELFPDHPRHFARWAKSSAHARATMTCHLDR
                     AYGASPGETLDIFPARKGDGSALMFIHGGYWRSLDKSDFSFLAPAWVDAGVSLVVVNY
                     DLCPKVSIERIVQQMLAASVWLYRHAEQYGMDEDRLYVSGHSAGAHLAAMMLAALWPV
                     MDRSLPKDLYKGAVAVSGVYDLRPLVQVDWLNGDLRLDEESALKLSPAYLPPATRAPL
                     ALAVGGLESSEFKRQNALLAQRWKPVLAGSIPMPGTDHFTVVDGLGDPSNALFAGAKR
                     MMRLDR"
     gene            19524..20162
                     /locus_tag="AC16O9_12345"
     CDS             19524..20162
                     /locus_tag="AC16O9_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR6517443.1"
                     /translation="MKEFLSPARYIDSDHPAVVEFARAHARGASDLERAVSLYYAVRD
                     GVRYNPFLDFSRDTAFQGSACLEQGEGFCIGKAALLAACARAEGIPARVGFADVKNHL
                     TTPRLAERMGSDLFIYHGFTELWLEDRWVKATPAFNLDLCRKFRVKPREFDGRHDSIF
                     HPFDADERRHMEYLRDRGSFADVPVAEIQQAFRECYPGLYGLGRDAARETFA"
     gene            complement(20164..21285)
                     /locus_tag="AC16O9_12350"
     CDS             complement(20164..21285)
                     /locus_tag="AC16O9_12350"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR6517444.1"
                     /translation="MTMRKSPPFRADHVGSFLRPKALLDAREQHFKHNAITRAQLREV
                     EDRAIRDIVKRQEDWGLQGVTDGEFRRTYFHVDFLEQLEGVEVKGGIPVKFHGAAGDV
                     DFAPPVMHVTRKLKHVQPIQREDFKFLQAATRRMPKVTIPSPTMLHFRGGRGAISREA
                     YPDMEGFYEDVARCYRDEIRQLADAGCRYLQLDDTNLAYLCDARMREGARQRGDDPDE
                     LPRRYARLINAAIAGKPQDMAVGVHLCRGNFQSAWAAEGGYEPVAEVLFNELQVDAYF
                     LEYDDARSGDFSPLRFVPKGKIVVLGLVTTKLGALESKDELKRRIDQAAKLVPLEQLC
                     LSPQCGFSSTVHGNQLQVEAQGAKIRLVVETAREVWGDA"
     gene            21321..22532
                     /locus_tag="AC16O9_12355"
     CDS             21321..22532
                     /locus_tag="AC16O9_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR6517445.1"
                     /translation="MRELLEQSFRAAVAAADPLKILPPHLPRPPRGRTFVAAAGKAAA
                     SMALAVEQHWPRGAALSGLAITRYAHGLPTQRIRVLEAGHPVPDAAGEAGARELLESV
                     RALGPDDLLLCLVSGGGSSLLSLPAPGVPMERLKAMTQALLRSGAPIQEMNTVRKHLS
                     AIQGGRLAAACRAPVLALIVSDVVGDDPTHIASGPCAPDPSTVEDAKAVLAKYGIEAP
                     GPFAETPKPGDRLFARVENRVIATARRSLEAAAALFEARGIAPIVLGDDITGEASEVA
                     RDMAAKARSARRGSVQISGGECTVTIRGAGGRGGRCSEFLLALALALEGVPGVHAIAA
                     DTDGVDGSQDNAGAVITPDTLARAKALGLDGKKLLKANDSYGFFAALGDLVVTGPTRT
                     NVNDYRAILVG"
     gene            complement(22529..22657)
                     /locus_tag="AC16O9_12360"
     CDS             complement(22529..22657)
                     /locus_tag="AC16O9_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517446.1"
                     /translation="MSWFWIIGIAANVSLTVLAIVWVIRQGKPKRDAAQDDKDSAA"
     gene            complement(22689..23804)
                     /locus_tag="AC16O9_12365"
     CDS             complement(22689..23804)
                     /locus_tag="AC16O9_12365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6517447.1"
                     /translation="MRPRPFRLQVPDAAIADLRERLARTRWPDEPPLAPWSSGTSVAY
                     LKTLVEYWRNGFDWRAEEAKLNALPQFTVPIRGIDVHFIHVPGTGPNPMPLLLSHGWP
                     GSVLEFLKLIPLLREQFTVVAPSLPGYAMSFRPGQERFGVEEIAQVCAELMTLLGYER
                     FAAQGGDWGAFVCSRLGVAVPKRLIGIHLNLLAVRRDPKAVVESDPAQRQYLGELATF
                     LTEETGYQWIQGTKPQTLAFGLTDSPVGLAAWLVEKFRSWTDCDGNPENALTRDEMLA
                     DISLYWFTGAIGSSFWPYYARRHRPWPIPEGTTVDVPMGYAAFPKEILRPPRSLAAQT
                     YTDIRRWSVMEKGGHFAALEQPQALAREIRAFFASLG"
     gene            complement(23818..24225)
                     /gene="rplQ"
                     /locus_tag="AC16O9_12370"
     CDS             complement(23818..24225)
                     /gene="rplQ"
                     /locus_tag="AC16O9_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR6517448.1"
                     /translation="MRHGLGLRKLNRTSSHRLAMLRNMAVSLLRHEEIKTTLPKAKEL
                     RRVVEPMITLGKAPSLANRRLAFDRLRDRDIVGKLFEELGPRYAKRNGGYLRILKSGM
                     RKGDNAPMALVTLMDRPEPKADETKPAEEAKAA"
     gene            complement(24233..25216)
                     /locus_tag="AC16O9_12375"
     CDS             complement(24233..25216)
                     /locus_tag="AC16O9_12375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6517449.1"
                     /translation="MPQSGFLKPRIVDVQNLSPSHAKVTMEPFERGYGHTLGNALRRV
                     LLSSMPGYAPTEVQIAGVVHEYSTMDGVGEDVVDILLNLKGIVFRLHNRPEAILSLKK
                     KGEGPVTAADIEPSHDVEIVNPEHVVAHLSATGKLEMQIKVESGRGYVPGNMRFLPEE
                     TKGIGRIILDASFSPVRRVSYAVESARVEQRTDLDKLIMDIETNGSIEPEEAIRYAAR
                     VLVDQLSVFAQLEGTALPAEAPKSPTVDPILLRPVDDLELTVRSANCLKAENIYYIGD
                     LIQRSETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEHHRG"
     gene            complement(25232..25861)
                     /gene="rpsD"
                     /locus_tag="AC16O9_12380"
     CDS             complement(25232..25861)
                     /gene="rpsD"
                     /locus_tag="AC16O9_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6517450.1"
                     /translation="MARDLDAKCRQCRREGEKLFLKGEKCFTDRCAVERRGYAPGQHG
                     QKSGSRMSDFGKQLREKQKLRRTYGVLERQFRRTYAEASRSKGVTGERLLQLLETRLD
                     NVAYRMGFGASRTEARQVVRHNGIQVNGRRVNIPSYQVRPGDVVQVTGKAKEQLRIKA
                     SSEAAEGRGFPEWLEVDAKALKGTFKSLPARTDLPSTVNESLIIELYSK"
     gene            complement(25883..26308)
                     /gene="rpsK"
                     /locus_tag="AC16O9_12385"
     CDS             complement(25883..26308)
                     /gene="rpsK"
                     /locus_tag="AC16O9_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR6517451.1"
                     /translation="MASRTTPTQQQLQQTASARARKKAKKNVAEGIAHIHASFNNTIV
                     TITDRQGNALAWATSGNAGFKGSRKSTPFAAQVAAERAGRAAQECGVKNIEVRIKGPG
                     PGRESAVRALNAIGLKIGSIADVTPVPHNGCRAPKRRRV"
     gene            complement(26324..26707)
                     /gene="rpsM"
                     /locus_tag="AC16O9_12390"
     CDS             complement(26324..26707)
                     /gene="rpsM"
                     /locus_tag="AC16O9_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR6517452.1"
                     /translation="MARIAGINIPNHQHAEIALTAIFGIGRARAQALCAAAGVERSRK
                     IKDLSDAEMERLREQVTRLTLEGDLRREVSMSIKRLMDLGCYRGQRHRKGLPVRGQRT
                     RTNARTRKGPRKAAIKLNVPAGKAA"
     gene            complement(26755..26868)
                     /gene="rpmJ"
                     /locus_tag="AC16O9_12395"
     CDS             complement(26755..26868)
                     /gene="rpmJ"
                     /locus_tag="AC16O9_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR6517453.1"
                     /translation="MKVMASVKKICRKCKIVRRKRVVRVICSDPRHKQRQG"
     gene            complement(26896..28218)
                     /gene="secY"
                     /locus_tag="AC16O9_12400"
     CDS             complement(26896..28218)
                     /gene="secY"
                     /locus_tag="AC16O9_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR6517454.1"
                     /translation="MATTLPSAGKAGRYGDLKRRLLFLLGALVVFRIGAHIPVPGIDP
                     NVLADLFKSQQGGILGMFNMFSGGALSRFTIFALGIMPYISASIIMQLMTAVSPHLDQ
                     LRKEGESGRRKITQYTRYGTVFLALFQATGISVALESQAGLVLDPGLMFRVTTVTTLV
                     TGTMFLMWLGEQITERGLGNGISLIIFAGIAAGLPSAIAGTLELVRTGAMHPLTALLI
                     AAAVLLVTAVVVFVERGQRKILVNYAKRQVGNRVYGGQSSHLPFKLNMAGVIPPIFAS
                     SLILFPATLLGWFGAAEGMVWLRDIAATLTPGQPIYVLMYAGLIIFFCFFYTALQYNP
                     KETADNLKKSGAFVPGIRPGEQTSRYLEKILTRLTLAGAIYVTLVCLLPEFLILRWNV
                     PFYFGGTSLLIIVVVTMDFMSQVQAYVMTHQYESLLRKANFKGGLPVR"
     gene            complement(28222..28686)
                     /gene="rplO"
                     /locus_tag="AC16O9_12405"
     CDS             complement(28222..28686)
                     /gene="rplO"
                     /locus_tag="AC16O9_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR6517455.1"
                     /translation="MRLNSLKPAPGAKKSRHRVGRGVGSGWGKTAGRGHKGQRARSGG
                     YHKVGFEGGQMPLHRRLPKRGFASPTRTHTAEVRLSELESMIAGDIDLGALKAQGIVP
                     REARMAKVILSGKLTRSVTLKGIKASKGARAAIEAAGGKVEDAAPAAQPAGE"
     gene            complement(28691..28873)
                     /gene="rpmD"
                     /locus_tag="AC16O9_12410"
     CDS             complement(28691..28873)
                     /gene="rpmD"
                     /locus_tag="AC16O9_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR6517456.1"
                     /translation="MANGKIKVTLVKSTAGCDADHRATVRGLGLRRIRQTVELVDTPS
                     VRGMIEKVSYLVRVEG"
     gene            complement(28882..29403)
                     /gene="rpsE"
                     /locus_tag="AC16O9_12415"
     CDS             complement(28882..29403)
                     /gene="rpsE"
                     /locus_tag="AC16O9_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR6517457.1"
                     /translation="MAKMKPRMQQSEERTDGLREKMVSVNRVTKVVKGGRVLGFAALT
                     VVGDGDGGIGMGKGKAREVPVAVQKAMEEARRKLFKVKLKNGTLHHAVIGHHGAATVL
                     MQPAHEGTGIIAGGPMRAVFEVMGITNVLAKCFGSTNPYNVVRATLQGLQAMNTPAEI
                     AAKRGKTVEEVTS"
     gene            complement(29413..29769)
                     /gene="rplR"
                     /locus_tag="AC16O9_12420"
     CDS             complement(29413..29769)
                     /gene="rplR"
                     /locus_tag="AC16O9_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR6517458.1"
                     /translation="MRDKNEARVRRARQTRLKIREIGAVRLTVHRTNSHIYAQLTSSS
                     GDKVLASASTLEKEVRAQVGNGGNRKAAETVGARIAEKAKQAGIEAVAFDRAGYRYHG
                     RVKALAEAARAGGLKF"
     gene            complement(29781..30314)
                     /gene="rplF"
                     /locus_tag="AC16O9_12425"
     CDS             complement(29781..30314)
                     /gene="rplF"
                     /locus_tag="AC16O9_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR6517459.1"
                     /translation="MSRIAKYPIALPKGVEVTLASGTISVKGPLGTMARPMDPNVGIQ
                     KEGETLVCKALGNSHHATAMSGTLRALVANMVTGVTKGFEKKLALVGVGYRAQAQGDK
                     LNLSVGFSHPVVHQMPKGVKVATPTQTEVVVSGIDKQLVGQIAAEIRDTKPPEPYKGK
                     GVRYANEVVVLKETKKK"
     gene            complement(30325..30720)
                     /gene="rpsH"
                     /locus_tag="AC16O9_12430"
     CDS             complement(30325..30720)
                     /gene="rpsH"
                     /locus_tag="AC16O9_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR6517460.1"
                     /translation="MSMTDPIADMLTRIRNAQMVGHAEVSMPCSKLKVSIAKVLKDEG
                     YIEDFVVGEDQPMKQLRIGLKYYAGRPVIERLERVSKPGLRVYKGRDDIPRVMNGLGV
                     AIVSTSRGVMTDRKARADGVGGEVLCIVA"
     gene            complement(30733..31038)
                     /gene="rpsN"
                     /locus_tag="AC16O9_12435"
     CDS             complement(30733..31038)
                     /gene="rpsN"
                     /locus_tag="AC16O9_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR6517461.1"
                     /translation="MAKLAVKLREQKRRQTVAKFKARRQALLDLIRDPRAQDEDREAA
                     REKLQKLPRDASPTRLRNRCAITGRPRGVYRKFGLGRNKLRDLALRGEVPGVIKASW"
     gene            complement(31049..>31197)
                     /locus_tag="AC16O9_12440"
     CDS             complement(31049..>31197)
                     /locus_tag="AC16O9_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR6517462.1"
                     /translation="VREQIIFPEIEYDKIDALRGMNIAITTTAKTDDEAKALLAAFRF
                     PFKH"
BASE COUNT         4570 a        11008 c        10701 g         4918 t
ORIGIN      
        1 tgctcgagcg ggtggtccat gcgctcccag ccgagctgcg tcgagccctt gcggcccgag
       61 tgcccgatct gcatgcagag cttcgccttg gaattggcgt ggcagaagtc ggcgatgcgc
      121 ttgaaggcgt cgcgctgcgc ctcgttccac aggcccgtgc acccgggcga gatgcgcgcc
      181 tcgggcgagg ggcaggtcat ctcgacgtag acgaggcccg cgccgccgac ggcgcggctc
      241 gcgaggtgca cgaagtgcca gtcggtgggc acgccgtcca ccgccgagta ctggtccatg
      301 ggcgagacca ccacgcggtt ctccaccacc atgtcgcgaa gccgcagcgg cgtgaacatc
      361 ggcaccggcg gattcttcat gtcgatgtcg aagccctgcg ccttaacctg cgccgcgaac
      421 cagcggtgca tggcctgcac gtaggcgggg tcgcggagct ccaggttctc ccaggtgatc
      481 tgcttggagc gcgacatgac gccgaaagcg aactgctcgg gcgccatgcc ccagtagcgc
      541 cgcatgtgct cgaaccaggc gagcgacacg ttcgccgcgt gctgcgtctt ctccacctcc
      601 tcgcgccgcg cggtgtcgta gcgcgcgagc gccggcttca ccccggcctc cgtgcgcagc
      661 gcctcgtaga gcgcgatcgc gtcctccatg gcgagcttgg tgcccgagcc gatggagaag
      721 tgcgccgtgg ccttggcgtc gccgacgagc accgcgttgc ccttcaccca ggtctggttg
      781 acgatggtgg ggaagttgcg ccagatcgag cggttggcga ggagcgggtg gccatcgagc
      841 tcctcggcga acacgcgctc cagcaccggc agcatctcgg cctcgcccat cttgtcgaag
      901 ccgaagttct tccacgtcgc ttcgtcggtc tcgatgaccc aggtcgagcg gccctcctcg
      961 tactggtagc agtgcgcaag gatgatgcct tcgggggtct cgcggaagaa gtacttgaag
     1021 gcgtcgagcg gccgggtcga gcccagccag gtgaacttgt tcggccgcag gtcgacgctc
     1081 ggcttgaagt gcgccttgtg ctgctcgcgg atcttcgagt tgatgccgtc ggcgaccacc
     1141 acgaggtcgg aatctgcgaa ctgctccagg ccctcgacct cggtctggaa gcggatggcg
     1201 acgccgagct cgcggcagcg gtcctgcagg attgcgagga gcgcgttgcg cgagcagccg
     1261 caaaagccgt tgccgcccga gcgcagcgtc ctgcccttgt acaccacgtc gacatcgccc
     1321 caataggcga agcgccgccg gatacgctcg tagctcgccg cgtcgtaggc cttgaaggtg
     1381 tcgagcgtct ggtccgagaa caccaccccg aagccgaaag tgtcgtcggg gcggttcctc
     1441 tcgtagcagg tgatgtccca gcgcggccag gccttcttcg ccagcaacgc gaagtaaagg
     1501 cccccggggc cgccgccgat gacggtgact ttcatctcaa tctcccggga cgacggcggt
     1561 ggcctcgatc tcgagccgcg ccgcatcctc caccaggccg ctcacctgca ctacggccat
     1621 caccggataa tgccggccca tcagctcgcg gtaggcggcg ccgacgtcct tgagagacgc
     1681 caggtactcg gccttgtcga gcacgtacca ggtgagccgc acgacgtgct cgggcctgcc
     1741 gcgcgcctcg gcgagcacgt cgaggatgtt ctgcagggcc tggcgaaact ggccggcgaa
     1801 gcccttctcc cgccactcgc cctggccggt gaagccgacc tgcccggcga tgtacacggt
     1861 ggtgccgccc ttcacggcga tgccgttggc atagcccctg ggtttcgccc agccgggcgg
     1921 ttgcaggatc gtcatcatgt cggggtcctc agcttgaagc gctgcagctt gccggtctcg
     1981 gtgcgcggca gcgcgtcgac gaactcgatg gcgcgcgggt acttgtaggg cgcgatggcg
     2041 ttcttgacgt gctcctgcag ctctttcgcc agcgcctcgg agccgcgcgc ctcgggcttg
     2101 agcacgacgt aagccttgac gatggcgccg cgctcggcgt ccggcgcgcc gaccacgccg
     2161 cactcggcca ccgccgggtg cgcaaggagc gccccttcta cttccggccc ggcgatgttg
     2221 tagccggcgg agatgatcat gtcgtcggtg cgcgcctggt agaagaagta gccgtcttcg
     2281 tccatgagat aggcgtcgcc ggtcacgttc cagccgttct gcacgtagtc cgtctgccgt
     2341 tcgtcggcga ggtagcggca gccggtaggc cctttcaccg ccagccggcc gatcacgccc
     2401 ggcctgcagg gccggcccgc ttcgtccagc accgtcgcgc gatagccggg gatggcgtag
     2461 ccggtggcgc cgcggcgcac gcgctcgggc gtgtgcgaga tgaagatgtg gatcatctcg
     2521 gtggacccga tgccgtcgat gatctcgatg ccggtcgctt gcttgaagag ctggcgcgtg
     2581 gcgtcgggca gcgcctcgcc cgcagagacg cacttcttga gcgcgccgag gtcgtagtcc
     2641 ttggccagcc ccgccatcgc gcggtacatg atgggcgcgg tgaagcacac cgtggccttc
     2701 tcgcgctgca aggtctcgag gagcgtctcc ggtgtcgcct tctccatcag cacggtgcag
     2761 gcgccgacgc gcatcgggaa cgcgagcagc ccgcccaggc cgaaggtgaa ggcgagcggc
     2821 ggcgtgccgc agaacacgtc gtccttggtg gcgccgaggc aggagcgcgg gaacgcgtcg
     2881 cacatggcga tcacgtcgcg gtgaaagtgc agcgtgccct tgggcttgcc ggtggtgccc
     2941 gaggtgaagg cgatgagcgc cacgtcctcg gcggcggtgt ccacgttggc gaacacctgc
     3001 ggcgagcgca cgcagagtgc gtcgagcgag tcgcgcgcgt cgtcgtgcca gtacttgacg
     3061 gccttcagcg tcgggcagcc cgcgcgcgcc gcctcgagct cggcggcgag ccgcttgtcg
     3121 cacagggcgt ggccgatctc ggccttgcgc acgatgtcgg tgagctcctt cgcgcgcagc
     3181 agcggcatgg tggccacgca caccccgccc gccttcatca ccgcgaacca gcacgccgcc
     3241 atcatcgggc tgttgggtcc gcgcagcagc acgcggttgc cgggcttcag gcccatgtcg
     3301 cgtaccagca cgttggcgat gcggttcgcc tgcgcgtaga gctccgtata ggtgcagctg
     3361 ccgtcgctcg cgcggatcac cgggcggtgg ccgaagccgc ggctcaccat cttgtcgagg
     3421 agctcggtgg cgcagttcat gcgcaccgga tagcggaact cgggccgctc cagcaggaac
     3481 tcgggccact ggctccgcgg cggcaggttg tcccgcgcga acgtgtcgag gtgggcgctc
     3541 ttgaccttca tttcagcagc tcgcgcgcga tgatcacctt ctgcacctcg gtggcgccct
     3601 cgtagatgcg cagcgcgcgc acctcgcggt acaggcgctc caccggatgg ccgcgcatca
     3661 cccccaggcc ccccaggatc tgcaccgcgt cgtcgatcac ctgctgcgcc gcctcggtgg
     3721 cctgcatctt ggccatcgcc gcctcgcgcg tcacgcgcgc ccgcttcacg tcccgcagcc
     3781 acgccgagcg gtacgtgagg agcgccgccg cctcgatcgc ggtcgccatg tcggcgagct
     3841 tcgcctgcgt catctgcagg tcggcgagcc gctggccgaa catcctgcgc tcccgggcgc
     3901 gggcgagcgc ctcgtcgagc ccgcggcgcg cgaagccgag cgccgccgcg gcgaccgtgg
     3961 tgcggaagac atcgaggttc ttcatcgcca gcttgaagcc ctggccttcg tcgccgatca
     4021 gcgtgcccct ggcgcccttg aacttcaccg tcgccatcgg gtgcggcgca acgatgtcga
     4081 tgcgctcgct ggcgtccgcg tccttcgcct cgacgacaaa cgcgctgatg cccttgtcgg
     4141 tcttggcgaa ggcgacgtag aagtcggcga tgccgccgtt cgagatccag gtcttcacgc
     4201 cgtcgagcac ccagtcggca ccctgcttct tcgcggtcgt cgtcatggct tgaacgtccg
     4261 acccggcatc gggttccgag agggcaaacg ccgctatcgc ctcgccagcc gccgcgcgcg
     4321 gcaggtagtc ccgcctttgc ttttccgacc ccgccagaac aatcgggccg ctgcccaggc
     4381 cctgcatgac gaaggaaaag tcggcgaggc cggcgtggta ggcgaggacc tccctgatga
     4441 gcgcgatgct gcgtacgtcc ggctgctcgt tgacgcagta cttcaggtag ccggcctgcc
     4501 ccaggtcctt gaccagtcgg cggcagatgg cgtccgcatc ctcgccatgc gcgtgcgccg
     4561 aatgggccaa ggcccaggtg tccaccgcac gcgataaggc cgcgtggggg ggatcgaaaa
     4621 aaggccaatc gaggtgggcg aagcggtcca tcaggaatgc tccagggttg tctgcttcag
     4681 gcgcgcgagc agctgcatca gcgcctcgga ttcctttctc gtcaggccgc cgaggagctc
     4741 gatcacccac tcctcgtgcg cgcgcgccat ctccgcgaac gccttgtcgc cggcgcgcgt
     4801 cagcgtcacg cgccacgcgc gccggtcttc ggcgtcgtcc acgcgctcga ccaggccctc
     4861 cttcaccagc tggtcgacga tcccggtgac gttgccgccg gtcaccatca gcaggcgcga
     4921 gagctcgctc atcttcaggc cccgcggatg gcgctcgagc tgcgacagca ggtcgaagcg
     4981 cggcagggtg gtgccgaagc gctccctcag ccgcgagcgc acgtggcgct cgatcagctg
     5041 cgagcaggtg agcaggcgca gccacagccg cagcgcgcgg tggtcgtcca ccgtggcgcg
     5101 ggtctcccta tccatcaggc ctgaatagct caagcctaaa gtatctgctt cgcgccggcg
     5161 cctggcaagc ccccgtcagc cgtgccaggc gcgccacagg aagctcgcgc caccgcccag
     5221 caccaccacg tccatgaacg cggtgtgcac cttgagcggc accagcgcca gcagccgccg
     5281 cgcgatgaag gcaccgggca tcgtgcacag gccgatcagc acgccggcga gcgcaagctc
     5341 ggcgtccagt cgcgccacgc tgccgaacag cacgatcttc gccacgccca tgaccaccga
     5401 gacgatcgcg tcggtggcga tgagcgatgc gccctccagc ccggcggcca tcagcatgga
     5461 gatgagcagc agtcccacgc cggtcatgct gccgttcacg aagccgaagc ccgcgcccgc
     5521 gatcgtgacg ccgcgcgcct cgagccgcca gccggcgcgc gcaagcaggc gccttatcgg
     5581 cacgctcacg atgaaaaagc tgccaaggag cagcgccacc cagcgcgctt cgaggagcgt
     5641 ataggcgtag gcgcccgcga cgcaggccgg caggcccatg atgaggatgc gccgcgcgtg
     5701 cggcttgtgg atgtcgcgcc agaaggcggc gacgcggctg ccgttcgcga agatcatgcc
     5761 gaccgccatc accggcacca cgttcaccac gccgaccacc ggcgcgagga agatcggcac
     5821 caccaggccc gtgccgtagc ccgccagccc gccgaggatc gacccggcga gcgcgaccgc
     5881 ggcgatcagg gcgagctcgg cggcgccgac gtcggcgagc tggaacgcgg cttccattcg
     5941 ggaatcgact cctgcaggaa ctgcagcact gcgacttcgt cgcggcgcag cgcgccgccg
     6001 gcgcgccccg cgcgcttcgc gcgcggcgga gccgccgacg gctggggcac ggtgcgcccg
     6061 agcaggtacg cgtgcagcag cgccgggtgc acgtagtact tgcggcacac ggcgcgcgtg
     6121 ttgccgaggc gctcggccac cgcgtcgatg gcgcgcagga tgttgcggtc ggcctcgcgc
     6181 cggctggccg ccggtcccat cgcgcgcagc tcgacagccg ccagcatggt gccgccccag
     6241 gtgcggaagt ccttcgccgt cacgtcgcga ccgctcgctt cgcgcaggta ctcgttgacg
     6301 tcgtccgagg tgaccgcctc gcgctttccc atcgcgttca ggtactggaa catctcctgc
     6361 ccgggcaggt cctggcagcg ctggatgatg cgcgcgaggc gcggctcggc aatcgagatc
     6421 gagtgctcca ccccgctctt gccgcggaac gtgaagcgca gctggttgcc gtccacctgc
     6481 acgtggcgcc ggcgcagcgt ggtgaggccg aaggagcggt tctcgcgcgc gtactcgtcg
     6541 ttgccgacgc ggatcagcgt acggtcgagc agccgcacca cggtggcgag cagctgcgcg
     6601 cgcccgagat cgtcgccgcg caggtcgcgc tcgacgcgct ccctgaggtg cggcagcgcc
     6661 tcgctgaact cgagcatgcg gcggaacttg gactggtcgc gcgcctcgcg gtaggacggg
     6721 tggtagcggt actgcttgcg gccgcgcgcg tcgcgcgccg tggcctggat gtgcccgccc
     6781 gggtcggggc agatccagac ctcggtccag gccggcggaa tggcgagcgc gttgaggcgc
     6841 ttgcgcgtcg ccgcgtccgc aatgcgctgg ccgtccggcg tgtagtaggc ccagccggtg
     6901 cccacgcgcc ggcgcgtgat gccggcgagg ccgtcgctga cataggtcag gcccgcgcgc
     6961 gtcgccgcgc ggcggtgctc gtgcgtgctc acggcagcgc gccgatggcc gagagcgccc
     7021 ggcgcacttc ctcgatccct tcgggcgcga gcggcgcctg cggcgcgagc ggcgcgccga
     7081 ccgcgtagcc ctgcagctcg agcgcgccct tgatgcacgc ggcgaggttg tacttggcga
     7141 aggcctggtt gagcgcccac agcgggcgct gcttttccat cgccgccgtc cactcgccgc
     7201 gccggcacag ctcgtagagc tcgacgctct ggcgcggcgc gacgcaggcc ggccctgcca
     7261 tccagcccac cccgcccagc agcatcacgc aggcgggcac gtgcgaggac gccgcgaaca
     7321 cctgcatgcg cccttcgacg cggttgagga tcgacagcag ccgcccggtg ttgacggagg
     7381 cgtccttgat gtagccgatg ttcggcaggc gcgacaggcg ttcgatcacc ggcagcgaaa
     7441 ggtccgagcg ctggaagttc gggttggtgt agagcacgac ggggatccgg acggcctccg
     7501 cgatcgccct gaagtaggcg tacacgccct catcgccgac ggggaagtag gcttcgagga
     7561 tggcgagtat gccgttgcag ccgatacgct ccatttcacg cgcctggaac accgcgtcgg
     7621 cgaccgtggt ggaggccacg ccggcgacga ccggcacgcg cccgcgcgcc gcctcgatca
     7681 ccacttcgac gacgcgccgg cgctgcgtcc agttcagata ggcgaactcc ccggtcgagc
     7741 cgagcggcgt gagcccgtgc acgcccgcgg cgatgaggtc gtcgcacagg cgcgcgagga
     7801 ccgtcgcgtc cacctcgccc gaggcgagca ccggcgacac gacgtacgga aagacgccgt
     7861 gaaagccggc ggccacgaat caggcctttc tgaggaaatc gaagtcgcag ccgtgctcgg
     7921 cctgcagcac gtggtccatg tacagcctgg cgtagccgcg cgcgggcatg gccgccggcg
     7981 gctgccacgc cgcctttctc tttgccagct cggcctccgg cacggcgagg tcgatgcgcc
     8041 gcgccttcac cgagatgcgg atgcgatcgc cgtgcttcac cagcgcgagc ggcccgccgg
     8101 ccgcggcctc gggcgagacg tgcagcacga tggtgccgta ggcggtgccg ctcattcgcg
     8161 catccgagat gcgcaccatg tccttcaccc cggcgcgcgc gagcttcgtc gggatcggca
     8221 gatagcccgc ttcgggcatg gcatagcccg acttcgggcc ggcgttctgc agcaccatga
     8281 aatcgtcggc ggtgacatcg agcgccggat cgtcgacgcg cgcggcgagg tcatcgagcg
     8341 agctgaacac caccgcgcgg ccctccttct cgaaaagccc cgggtccgcc gcggagcgct
     8401 tcaggattgc cccattgggc gcgagcgtgc caaagagcgc caccagcccg ccctgtttcg
     8461 ccagcggctc ggcgagcggg cgcaccacgg cgcggtccac ccaggcgcct ttctcctgct
     8521 cgagccgctc gcccagcgtc tccccggcca ccgtcatgca ctcgaggttg agcagcggct
     8581 tgagctcgcg caacaccgcg ccgacgccgc ccgcggcgta gaggtcagac atgtagtgct
     8641 ggcccgtggg cttgaggtcc accagcaccg gggtcgagtc cgacagctcg ttcaggcgct
     8701 tgagatccac ccggacgccg gcgcgcccgg cgatcgccgt gaggtgcacg atggcgttgg
     8761 tcgagccgcc gatggcgagc agcacgcgca gcgcgttctc caccgatttt tccgtgatca
     8821 cctgcgacgg gcggatcggg gccttgacga gctccaccgc gcggcgcccg ctcgcctcgc
     8881 ccgcgcgtag ccgatcggcg tgcactgcgg gaatcgccgc ggtgccgggc agcgccatgc
     8941 cgagcgcctc cgcgatcgaa gccatggtgg aggccgtgcc catcaccgcg caggtgcccg
     9001 cggtggtggc gaggttgccc tcgacctcgc cgatgcccgc cgcatcgact ttgcccgcgc
     9061 ggtgcagcgc ccagaagcgc cggcagtcgg tgcaggcgcc gagccgctcg ccgtgaaacg
     9121 aggtcggcat catgggcccg gcgagcagct gcaccgccgg cacgtcggcc gaggccgcgc
     9181 ccatcagctg cgcgggcacc gtcttgtcgc agccgccgac cagcaccacc gcgtccatcg
     9241 gctgcgcgcg gatcatctcc tcgacgtcga tcgacatcag gttcctgaac atcatcgagg
     9301 tggggctcag gaacacctcg cccagcgaca ccgtcgggaa ctcgatcggc aggccgcccg
     9361 cggcgagcac gccgcgcttc accgcctcga tcagctcggg gaaatgccga tggcagttgt
     9421 tgaagccgct cttggagtcc gcgatgccga ccacgggccg ctcgagcatc tcgcccgagt
     9481 agcccatgct cttcgcgaac gagcgccgca ggtacagcga gaagcccgca tcgccgtagt
     9541 tggtgaggcc ccgtgcgagg ccctgcggct tgttcgcgct catcgggggc tcagccctgc
     9601 accaccgctt cgacgtagat ctcgacgcgc cggttggcct ggcggcccgc ctcggacgcg
     9661 ttgctcgcca ccggcacggt ctcgcccttg cccgcggtgg cgaggcgcat gccgttcacg
     9721 tttcgcgact cgaggtactg cgccaccgac agcgcgcgct cctccgagag cttctggttg
     9781 taggtgttcg atccggtgct gtcggtgtgc ccgaccacgg tgagggtggt cttgccgtag
     9841 cgcacgacga cgtcggcgag cttgtcgagc gtgctgcgga agcccgggtt gacgttggtc
     9901 gaatcgacct cgaacgcggt ctggctggtc atggtgacca ggacgacctg ctcggagagc
     9961 ttctgcacgc gcacctgccc ggcctggatc tcgggcgcca ggttcttttc caggtcgcgc
    10021 ttctggccgt ccatgtagtt gcccacggcc gcgccggcaa gaccgccgcc gatcgcgccc
    10081 accaccgcgc ccttggtgcg gttcttcttg taggccatgg cgcccaccac ggcgccgccg
    10141 agcgcgccga tcatcgcgcc gctctgcgtg cgcgtcatgg gtcggttggg atcggtctgg
    10201 cacccggcga gcaacgcccc gcccacgatc atcgctatcc acgatttctt catgtcacct
    10261 ccgtctcttg catcccctcc agcccccaga cgcaagggta ccccatcgcg cgcgtggcgc
    10321 cggcctaggc taagcgcgcg ctgtactcgg agaagatgcc gagctgcacg ccgtgcgcaa
    10381 cccgcgtgaa gccggccgcc gagaggctcg ccatgatgcg cgcgggcggc acgcaggcct
    10441 cgatcgtgtc ccagtagtac cggtacagtg ccggcgtatc gcggctcgcc gccagcacgc
    10501 gcgccagcgc cggcaccacg ccgcgcaggt aggccttcag cgccgcggcc gcgaggcgtc
    10561 cggccggccg ggtgatctcg agcatcagga gctgcccgcc gggcttgagc acgcgccgga
    10621 actcggcgaa cacggcgccg aggtccgcca ggtgtcgcag cgcgtagccc aggcagagaa
    10681 aatcgaacga ctgcgccgcg aacggcagcg cttccgcgcg cgcgcgcgcc agcgtgagcc
    10741 cgtgcgcttc cgattgcgcc agcatgcccg cgctcgggtc gacgccgatc acgtaaccgc
    10801 gcgccccgac cacgccgagc gcctcgcgcg cgaccaggcc cgtgcccacc gcgacgtcga
    10861 gcatgcgcat gcccggcgcg agccccgcgc gcagcagcgc ctggcggcgg taccaggagc
    10921 cggagccgag cgcgagcagc cggtcgacgc gatcgtagtc gggcgcgctc gaatcgaaca
    10981 tgcgccgcgt gaagcgccgc cgggcgtcgg tttcggcaac actcgccgga tgcggatcca
    11041 tgggttcgac cttggccacc gaggcgatgg tacagtctcc acgcccaatc cgaggagcgc
    11101 gccatggccg caccgaaatc cccgcccacc acgatcggcg ccgtcaagga catcgtcccc
    11161 aagcttgccg agctcaccga gaaggtcgtg ttcggcgacg tctgggcgcg gcccggcctg
    11221 tccaagcgcg accgcagctt cgccacggtg gcggcgctga tcgcgctgca ccggcccgat
    11281 cagctgaagg gacacatctg gcgcgcgctc gacaacggcg tgacgcgcga ggagcttgcg
    11341 gagctcttca cgcacctcgc cttctacgcc ggctggccga acggcatcag cgcggcgcag
    11401 gtggccaagc aggtctacga ggagcgcggc gcatgaaggc cggcttcgtc gggctgggcg
    11461 tcatgggcgc gcatatggcc gagaacctgg cgcgcgccgg ccacgcggtg atcgggaccg
    11521 acctgcgcaa ggtggaggtc gcgggcgtcc gatgggcggc cagcgcggcc gaagcggccg
    11581 ccgaggccga cgtcgtgttc acgtcgctcc ccggcccgaa ggaggtcgag gcgctggcgc
    11641 ccaagctcgg cgacgccctg ccgaagggcg ccgcctggtt cgacctcacc accaactcgc
    11701 ccgcgtgcgt gcgccgcgtg cacgcgcagc taaagacgct cggcgtcgat ttcctcgatg
    11761 cgcccgtgag cggcggcaag gcgggcgcgc agagcggccg gctcgcgatc tgggtcggcg
    11821 gcgacgaggc cgtctaccgg cgcttcgagc ccatgctgcg cgcgatcggc gaccagccgt
    11881 tctacgtcgg cccgatcggc gcgggctcga tcgccaagct cgcgcacaac tgcgccagct
    11941 tcaccgtgca gaccgcgctc gccgagatct tcaccctcgg cgtcaaggcg ggcgtcgagc
    12001 cgctggcgct cttccaggcg ctgcgccacg gcgccaccgg caggaagcgc acgttcgacc
    12061 gcctcgcgga gcaatacctg ccgggcgcct atgacccgcc cacctttgcc gcacgcctcg
    12121 cgcacaagga cctgtcgctc gcgctcgagc tcgcgcgcga ggtgggcgtg ccgatgcagg
    12181 ttgccgagcg cgcttttgcg gatttcagcg aagccctcaa gcgcggctgg ggcgagcgcg
    12241 actcgcgcac gccgatgctg ctgcagaacg agcgcgcggg ggtcgacccg cacgtgccgg
    12301 cggcgaagct gcgcgaggtg ctggagaagg gctagaagcg gtagcgcagc tcgatgccgg
    12361 tctcgcgcgt cgtgttggca ggctcgagcc cgacgaaacc catgccgatc ggctgaatgt
    12421 cggagtcctc gatcttccag tagtgcgacc acaggctcac ggaccagcgc gggctctcaa
    12481 gcgccagctc gccgcgcgcg ccgcgcccgc gccgctgatg gttagtgacg gcgttgaagc
    12541 ccagtccggt gtcgctcagg tagctgcgct gcttgccatg cacaaaggcg tcgtactcga
    12601 tccgcggcgc cagcacccag cccgcattca tgggaaagcg cagcgccacg ccgacgggaa
    12661 tgtagtaata gcgcgatttg cggcgatagc cgagcgcgcc cgtgctgctc acgccgcgca
    12721 ggtcgttgta gaggtagcgg tagccaaggc cggcgtaagg cacccaaacg aagcgcccgg
    12781 cgcggtagtc ccggccaacg agcgcgcgca actcgagaat gtagtcctcc acgtcgcgca
    12841 acgttcccga gcccgtgtag tcgagctcgg cgtaggagta gcgcccctcg atgtgatgat
    12901 ggatgtagtc ctgtccgagg aaggtcaccg agcccgtggc gccgacgcgc tcgccctcaa
    12961 gcagcgcgaa gttgggctcc tcgtattcgt aggtcgagcc gagcagcccg acttcccagc
    13021 cgccgcgcgt gccgagcggg tcgcgctgcg ccgcggccga cccgagccac agcaccgccg
    13081 ccagacccgc caccgccgct gtccccgcac gcatgccagt aatatagcaa caggaggagc
    13141 acgccatgcg tctgcattcc cgcctgcgct tcgcgccggc cgaggtccgc gtcgacaagc
    13201 gcgccgacgg ctcgacgctg ctgcgctcgc cccaggcgct gcgcgcgcac gagcgctgcg
    13261 tcggcgagtg gctcgtcaag tgggcgcggc agagcccgga gcggcccttt ctggccgaac
    13321 gcgcgggcga gggttggcgc aaggtcagct acgcgcaggc gctcgatgcg gcgcggcgca
    13381 tcggccaggc gctgcttgag cgcggcctcg ggcccgacaa gccgctcgcc atcctctccg
    13441 acaacagcgt cgaccacggc ctgctcgcgc tcggcgccat gcacgccggc gtgccggtcg
    13501 cgcccgtctc gcccgcctac tcgctcatgt ccaaggactt cgcgaagctg aaggcgatct
    13561 tcgagctcgt gaagccgggg ctggtgtggg tggacgaccc gaagaagtac gccgccgcgc
    13621 tcgccgcggc cggcgccgag cccacgccgc tcgccgcgct cctggagcgc gcgcctggcg
    13681 cgcgcattga cgaggccttc gcgacgctgg gccccgatac cgtggcgaag atcctgttca
    13741 cgtccggctc gacgggcctg cccaagggcg tgatcaacac gcagcggatg ctcgccgcca
    13801 accagcagat gatcgcgcag gtatggccgt tcctcgagga ccgcgtgcag accatctgcg
    13861 actggctgcc ctggaaccat accttcggcg gcaaccacaa cttcaatctc gtgctgcgca
    13921 acggcggcac gctctacgtg gacggcggca agccggcgcc cgggctggtc gaggtcacgg
    13981 cgagaaacct cagggaagtc gccggcacga tgtacttcaa cgtgccgcgc ggcttcgacc
    14041 tgctgctgcc gttcctggag aaggacgccg agctgcggcg caacttcttc cgcgacctcg
    14101 acgtgctgtt ctacgccgcc gcggcgctgc cgcagaacct gtgggagcgg ctggagaggc
    14161 tcgcgctcga ggaaaagggc ggcgagctcg ccatgctctc ggcctggggc tcgaccgaga
    14221 ccgcgccctc ggcggccgcg gtgcactatc acatcgagcg cgcgggcgtg atcggcctgc
    14281 cgaacccggg ctgcgagctg aagctcgtgc ctgccgccgg caagctcgaa gtgcgcgtgc
    14341 gcggacccca cgtcacgccg ggctacttcc ggcgcgacga tctcacgcgc gccgcgttcg
    14401 acgaggaagg cttctaccgc atcggcgatg cggtgaagtt cgccgacccg caggcaccgc
    14461 agaagggcct ggtgttcgac ggccgcgtcg ccgaggactt caagctcacc tcgggcacct
    14521 gggtgcacgc gggcgcggtg cgcgtgaagc tgatcgccgc gggcaatccc tacatccagg
    14581 acgcggtgat caccgggcac gatcgcgacg aggtcggcgc gctcgtcttt ccgagcccgg
    14641 cggcgaagga cctcgcgccc gaggcgctgc gcgcgaagat ctcggcggcg ctgaaggcgc
    14701 tcggcgccga gggcggaacc tcgatgcatc cgacgcgcgc gctcgtcacg gccgatccgc
    14761 cctcgatcga cgccaacgag atcaccgaca agggctacat caaccagcgc gcggtgctgg
    14821 agcggcgcgc ggcgctggtg gagaagctgc acgcgaagcc tccgggcgcc gaggtcatca
    14881 ccccggcgcg ctagggcgcc atcagccgcg gcagccagag cgtaagctcg ggcaccgcga
    14941 cgagcagcgc gaccacggcg tgggaaagag cctgaacgtg cgcgccgtca cgtccaggca
    15001 gaccagcacc accagggcgc acagcagcgc ccccgcgagg atggcgagcg cctcgaccag
    15061 ccggtcgagc gccgcgcgcg ccgctgccgc cgccgcggac cacgactctc atctggtata
    15121 tcgtatggac atggagcgct ccttcaggga agaggtcgcg aaactgcggc tcggcgacgg
    15181 cgagaccttc cacggcgaag gcatcctcgc ggtcaccaag gcgctgctgc agtcgggcgt
    15241 gtcctacgtc ggcggctacc agggcgcgcc ggtctcgcac atgatggacg tgctgaacga
    15301 cgcgcgcgac atcctcgacg agctcggcgt gcacgtcgag accaacgcgt cggaggccgg
    15361 cgcggcggcg atgctcggcg cctcgatcaa ctacccgatg cgcggcgcgg tgaccttcaa
    15421 gtccaccgtc ggcaccaacg tcgcgtccga tgcgctctcc aacctcgcct ctgccggcgt
    15481 caagggcggc gcgctgatcg tgctcggcga ggactacggc gaaggcgcct ccatcatcca
    15541 ggagcgcagc cacgccttcg ccatgaagtc gcagatctgg ctgctcgatc cgcgcccgag
    15601 cctgccgcgc atcgtcgaga tggtggagca cggcttcgag ctctcggaag cctcgagcac
    15661 gccggtgatg ctggagctgc gcatccgcgc ctgccacgtg cagggcagct tcgccgccag
    15721 gcgcaaccgc gccccgcgca tttcgcgccg cgcgctgctt gagaatccgg acttcgactt
    15781 cgcgcgcatc agcctgccgc cctccacgta cgcgcaggag aagcacaaga tcgacgtgcg
    15841 ctggcccgcg gccgtgcgct acatccgcga gcaggggctg aacgaggcgt tcccgggtga
    15901 gcgcgccgac atcggcatcc tgtgccaggg cggcatgtac aacgccgtga tccgcgcgct
    15961 gcagcagctc ggcctcgccg acgccttcgg cgccgcgcgc atccccatct actgcatgaa
    16021 cgtgacctac cccctggtgc cggaagagat cgtcggcttc tgcgccggca agaacgccgt
    16081 gctggtggtg gaggaaggcc agccggcgta cctcgaggac gcgatcctcg ccacgttgcg
    16141 ccgccacgac gtcggcgcgg tcaaggtgca cggcaaggac gtgctgccga tggcgggcga
    16201 gtacaccggc gaagtcgtgg tgacgggtct ctcgaagttt ctcggcgccg aagaggtgct
    16261 ggcacggatc acggcgccca agctgaaggc cgcagcgctc cttggcgcgc cgctgcccac
    16321 gcgcccgccg ggcttttgcg tgggctgccc cgagcgcccg gtgttcgcgg ccatgaagct
    16381 cgtggaaaag gaactcggca agtaccacgt ctcggccgac atcggctgcc acaccttctc
    16441 cacgctgccg ccgttcaaca tcggccacac ggtgctcggc tacggcctcg ggcttgcgtc
    16501 caactcggcg gtgcagccga tgttcggccg gcgcaccgtg accatcatgg gcgacggcgg
    16561 cttctggcac aacggactca cctcgggcgt ggcgaacgcc gtgttcaaca aggacgacgg
    16621 catcctcgtc atcatgaaga acggctacag ctcggccacc ggcacgcagg agctgccttc
    16681 cagcccccgg cacagcgagc acaaggcccc ggacatgtcg atcgagcgcg cgttgcaggg
    16741 cctcggcgtg ggctggatgc gcacggtgca cacctatcac gtcggcgaga tgaagaagac
    16801 cctgaaggac gcgatgacca ccggctacgc tggcctcaag gtcgtgatcg ccgacggcga
    16861 atgccagctc gagcgccagc gccgcgtgcg cccggtgacc gcgaaacagc tcgccgccgg
    16921 cgagcgcgtg gtgcgcgtca agtacggcgt ggacgaagac acctgcaccg gggatcattc
    16981 ctgcatccgg ctctcgggct gcccgacgct caccgtcaag gacaaccccg atccgctgcg
    17041 ccaggacccc gtggccacgg tcatcgaggg ctgcgtcggc tgcgggctgt gcggcgagaa
    17101 cgcgcacgcg gccacgctgt gcccgtcgtt ctaccgcgcc gaggtgatcc agaacccatc
    17161 gctcgtcgac cgcaccctgg ggcgcctgcg ccgcctggta gtgccggcat gagcctgccc
    17221 gagcgctcgg tcacggtgct catcgccgcg ctcggcggcg aaggcggcgg cgtcatggcg
    17281 gactggctga tgcacgcggc aactgccagc ggcttcccgg cgcaggcgac gtcgattccc
    17341 ggcgtcgcgc agcgcacggg cgccacgacc tactacctgg agatctttcc cgcgcagcgc
    17401 gctcagctcg gcgggcgcga gcccgtgatg tcgctcacgc cgagcccggg caacgtggac
    17461 ctgatggtcg cctccgagct gctcgaggcc gggcgcgcga tgcagaacgg ttttgtcgcg
    17521 ccgcagcgca ctacgctcat cgcctcgacg caccgcatct acgcgaccgt cgagaagatg
    17581 cagatggcgg acggccgctt tcccgaggag cgcgtgctgt ccgcggcgag gcagctggcg
    17641 aagcatgccg tgctgttcga catgcgccgc atggccgagg agaacggcac cgtgatcaac
    17701 gccgtgctgt tcggcgccat ggccggctcg ggcgcgctgc cgctgccgcg cgaagcctgc
    17761 gagacggcga tactgcgcgc gggaaagggg gcgcaggcca gcctacgcgg cttcgccgcc
    17821 ggctatgagg tcgcggccgg cgcgcgtccg gcgccgcccg cagcggcgcg gcccgtacct
    17881 cccgagacgc gccagatcct cgagctgggc gcggcgcgcc tgcgcgacta ccagggcgag
    17941 gcctacgcca ggctgtacct ggagcgcatc gccgctctgg gcaacgccga cgcgaagctc
    18001 gtcggcgaga ccgggcgcca gctcgcgctg tggatgagct acgaggacat cattcgcgtt
    18061 gccgacctga agacgcgcgc ctcgcgcttt gcgcgggtgc ggcgcgaagt gggcgcccgg
    18121 gacgcggagc ccgtggtggt gacggactat ctcaagcccg gtgtggagga ggtggcttcg
    18181 cttctgccgc ccgcgctcgg ccggcgagtg cgcgcctggg ctgaacggcg cggcaagctc
    18241 gacgcctaca acgtcggcat gcacatacgc acctccagca tcggcggcta cctgctggtg
    18301 cgctcactcg ccgggctcaa gccgtggcgg ccgcggagct atcgctatgg cgaggagcag
    18361 gcgctgatcg agcgctggct cgccgccgtg cgcgccgccg cgccgcacag cagcgcgctc
    18421 gcgctggaga tcgtggcctg cgccggcctg ctgaagggct acggcgagac gcaccgccgc
    18481 ggccgtgcga gcttcctcgc catcctcgat gcgctggtgg agaacccggc ggcgggcgac
    18541 gccgccgcgc gcgttgcggc catccgcaag gcgcgcgagg cggcgctcgc cgatccggaa
    18601 cagaaggcgc tggcgaaggc gctgggccgg cccgtcacct ggctgcacgc ggccaagacc
    18661 cgctagaatc gcgggttcat gaccgatccg gcctttctca gccgcgagta caacaaccgc
    18721 gagctgttcc cggaccatcc ccggcatttc gcgcgctggg cgaagagctc ggcgcacgcg
    18781 cgcgccacga tgacctgcca tctcgaccgc gcctacggcg cctcgcccgg cgagaccctg
    18841 gacatcttcc cggcgcgcaa gggcgacggc agcgcgctca tgttcatcca cggcggctac
    18901 tggcgctcgc tcgacaagag cgacttctcg tttctcgcgc cggcgtgggt ggacgcgggc
    18961 gtgtcgctgg tggtcgtcaa ctacgacctg tgccccaagg tgagcatcga gcgcatcgtg
    19021 cagcagatgc tcgccgcctc ggtatggctc taccggcacg ccgagcagta cggcatggac
    19081 gaggaccgcc tctacgtgag cggccactcg gcgggcgcgc acctcgcggc gatgatgctg
    19141 gcggcgctgt ggccggtgat ggaccgctcg cttcccaagg acctgtacaa gggcgcggtc
    19201 gcggtgagcg gcgtctacga cctgcggccg ctcgtgcagg tggactggct gaacggcgac
    19261 ctgcggctcg acgaggagag cgcgctcaag ctctcccctg cgtacctgcc gccggccacg
    19321 cgcgcgccgc tggcgctcgc ggtgggcggg ctggaaagct cggagttcaa gcgccagaac
    19381 gcgctgctgg cgcagcgctg gaagccggta ctcgccggca gcattccgat gccgggcacc
    19441 gaccacttta ccgtcgtcga cggcctcggc gacccctcga acgcgctgtt tgccggcgcc
    19501 aagcgcatga tgcggctcga ccgatgaagg aattcctctc ccccgcgcgc tacatcgaca
    19561 gcgatcatcc ggcggtggtc gagtttgcgc gagcgcatgc ccggggtgcg agcgacctcg
    19621 agcgcgcggt gtcgctctat tacgcggtgc gcgacggcgt gcgctacaac ccgttcctgg
    19681 acttttcccg cgacaccgcg ttccagggca gcgcgtgcct cgagcagggc gagggctttt
    19741 gcatcggcaa ggcggcgctg ctcgcggcct gcgcgcgcgc cgagggcatt ccggcgcgcg
    19801 tgggcttcgc cgacgtcaag aaccatctca ccacgccgcg cctcgccgag cgcatgggct
    19861 cggacctctt catctaccac ggcttcaccg agttgtggct cgaggacagg tgggtgaagg
    19921 cgacgccggc cttcaacctc gacttgtgca ggaagttccg cgtcaagccg cgcgagttcg
    19981 acggccgcca cgactcgatc ttccatccgt tcgacgcgga cgagcgtcgc cacatggagt
    20041 acctgcgcga ccgcggctcg ttcgccgatg tgccggtcgc cgagatccag caggcgttcc
    20101 gcgagtgcta cccggggctg tacggcctgg ggcgcgacgc ggcccgcgaa accttcgcct
    20161 gagctaggcg tcgccccaga cttcgcgcgc ggtctcgacc accaggcgga tcttcgcgcc
    20221 ctgcgcctcg acctggagct ggttgccgtg caccgtgctg gaaaagccgc attgcggcga
    20281 aaggcacagc tgctccaggg gcacgagctt cgccgcctga tcgatgcgcc gcttgagctc
    20341 atccttcgat tccagcgcgc cgagcttggt ggtcaccagc ccgagcacga cgatcttgcc
    20401 tttgggcacg aagcgcaagg gagagaagtc gcccgagcgg gcgtcgtcgt actcgaggaa
    20461 gtacgcgtcc acctgcaact cgttgaacag cacttcggcc accggctcgt agccgccctc
    20521 ggccgcccag gcgctctgga agttgccgcg gcaaaggtgc acgccaaccg ccatgtcctg
    20581 gggcttgccg gcgatcgcgg cgttgatcag gcgcgcgtag cgccgcggca gctcgtccgg
    20641 atcgtcgccg cgctggcgcg cgccctcgcg catcctggcg tcgcacaggt aggcgaggtt
    20701 ggtgtcgtcg agctgcaggt agcggcagcc ggcgtcggcg agctggcgga tctcatcgcg
    20761 gtagcagcgc gccacgtcct catagaagcc ctccatgtcg ggataggcct cgcggctgat
    20821 ggcgccgcgc ccgccgcgaa agtgcagcat ggtcggcgac gggatagtga ccttgggcat
    20881 gcgccgcgtc gccgcctgga ggaacttgaa atcctcgcgc tggatcggct gcacgtgctt
    20941 cagcttcctg gtcacgtgca tcaccggcgg cgcgaagtcg acgtcgccgg cggcgccgtg
    21001 gaacttgacc gggatgccgc ccttcacctc gacgccctcc agctgctcga ggaagtcgac
    21061 gtggaaatag gtgcggcgga actcgccgtc ggtgacgcct tgcaggcccc agtcctcctg
    21121 ccgcttgacg atgtcgcgga tggcgcggtc ctcgacttcg cgcaactgcg cgcgcgtgat
    21181 ggcgttgtgc ttgaaatgct gctcgcgcgc gtcgagcaac gccttgggtc gcaggaagct
    21241 gccgacgtgg tcggcgcgga acggtgggct ctttcgcatc gtcatggcgc tgcgagcata
    21301 gcgcaaatac actgcggctg atgcgcgaac tcctcgagca aagcttccgc gcggcggtcg
    21361 ccgcagccga tccgctgaag atcctgccgc cgcacctgcc gcgcccgccg cgcgggcgca
    21421 ccttcgtcgc cgccgccggc aaggcggcgg cctcgatggc gctggcggtg gagcagcact
    21481 ggccgcgcgg cgcggcgctc tcggggctcg ccatcacgcg ctacgcgcac ggcctgccca
    21541 cgcagcgcat ccgcgtgctg gaggccggcc atccggtgcc ggatgccgcg ggtgaggccg
    21601 gcgcacgcga actgcttgag agcgtgcgcg cgctcggacc agacgacctg ctgctgtgcc
    21661 tggtgtcggg cggcggctcg agcctgctgt cgctgcccgc gccgggggtg cccatggaga
    21721 gactgaaggc catgacgcag gcgctgctgc gcagcggcgc cccgatccag gagatgaaca
    21781 cggtgcgcaa gcacctctcc gcgatccagg gcgggcggct tgccgccgcc tgccgcgcgc
    21841 cggtgctcgc gctgatcgtg tcggacgtcg tcggcgacga ccccacgcac atcgcctcgg
    21901 gcccctgcgc gcccgacccg agcacggtcg aggacgcaaa ggcggtgctc gccaagtacg
    21961 gcatcgaggc gccggggccg ttcgccgaga cgcccaagcc cggcgaccgg ctattcgcgc
    22021 gggtcgagaa ccgcgtcatc gccactgcgc gccgctcgct cgaggccgcg gcggcgctgt
    22081 tcgaggcgag gggcatcgct cccatcgtcc tcggcgacga catcaccggc gaggcgagcg
    22141 aggtggcccg cgacatggcg gcgaaggcgc gttccgccag gcgggggtcg gtccagatct
    22201 ccggcggcga atgcacggtc acgatccgcg gtgcgggcgg ccgcggcggg cgctgcagcg
    22261 agttcctgct ggccctcgcg ctcgcgctgg agggcgtccc gggcgtgcat gccatcgccg
    22321 cggacaccga cggcgtcgac ggctcgcagg acaatgccgg cgccgtcatc acgccggata
    22381 cgctggcgcg ggcgaaagcg cttggcctcg atggcaagaa gctgctcaaa gcgaacgaca
    22441 gctacggctt cttcgcggcg ctcggcgacc tggtggtcac cggcccgacg cgcaccaacg
    22501 tcaacgacta ccgcgcgatc ctggtcggct aggcagcgga atccttgtcg tcctgggcgg
    22561 cgtcgcgttt cggcttgccc tggcggatga cccatacgat ggcaagcacc gtaaggctca
    22621 cgttggcggc aatgccgatg atccagaacc agctcatggc gaattggggg cgggcatcga
    22681 gccgcaacct agcctagcga agcgaagaac gcgcggatct cgcgcgccag cgcctgcggc
    22741 tgctccaggg cggcaaagtg cccgcccttc tccatcacgc tccagcggcg gatgtcggtg
    22801 taggtctgcg ccgccaggct gcgtggcggc cgcaggatct ctttcggaaa cgcggcgtag
    22861 cccatgggca cgtccaccgt cgtgccctcc gggatcggcc acggccggtg cctgcgcgcg
    22921 tagtagggcc agaacgacga gccgatcgcg ccggtgaacc agtagagcga gatgtccgcg
    22981 agcatctcgt cccgagtgag cgcattctcc gggttgccat cgcagtcggt ccagctgcgg
    23041 aacttctcga ccagccaggc tgcgaggccc acgggtgaat cggtgagccc gaaggcgagc
    23101 gtctgcggct tggtgccctg gatccactgg tagccggtct cttcggtgag gaaagtcgcg
    23161 agctcgccaa gatactgcct ctgcgccgga tccgattcga ccaccgcctt cgggtcgcgc
    23221 cgcacggcga gcaggttcag atggatgccg atcagccgct tggggaccgc caccccgagg
    23281 cgcgagcaga cgaacgcgcc ccagtcgcca ccctgcgccg cgaatctctc gtagccgagg
    23341 agcgtcatca gctcggcgca gacctgcgcg atctcctcga cgccgaagcg ctcctgcccg
    23401 ggcctgaacg acatcgcgta gccggggagc gagggcgcga cgaccgtgaa ctgctcgcgc
    23461 agcagcggga tcagcttgag gaactcgagc accgagcccg gccagccgtg cgagagcagc
    23521 agcggcatag ggttcggccc ggtgcccggc acgtggatga agtgcacgtc gatgccgcgg
    23581 atcggcacgg tgaactgcgg cagcgcattc agcttcgctt cctcggcgcg ccagtcgaag
    23641 ccgttccgcc agtactcgac cagcgtcttg aggtaggcga cgctggtgcc gctcgaccag
    23701 ggcgcgagcg gcggctcgtc cggccagcgc gtgcgcgcaa ggcgctcgcg cagatcggcg
    23761 atcgcagcgt cggggacctg caggcggaaa ggacggggcc gcatccccgc gcgcgcccta
    23821 cgcggccttc gcttcctcgg ccggcttcgt ctcgtcggcc ttcggctcgg gccggtccat
    23881 cagcgtcacc agcgccatcg gcgcgttgtc gcccttgcgc atgccgctct tcaggatgcg
    23941 cagatagccg ccgttacgct tcgcgtagcg cggaccaagc tcctcgaaca gcttgccgac
    24001 gatgtcccgg tcgcgcaggc gatcgaaagc aagccgacgg ttggcgagcg aaggcgcctt
    24061 gcccagcgtg atcatcggct ccaccacgcg acgcagttct ttcgccttgg gcagcgtcgt
    24121 cttgatttcc tcgtgccgca gcagcgacac cgccatgttg cgcagcatcg cgagccggtg
    24181 gctcgaggtg cggttgagct tcctgaggcc tagtccgtga cgcatggcgc cctcatccgc
    24241 ggtgatgctc gagcccggcc ggcggccagt tctcgagctt catgcccaga gtaagcccgc
    24301 gcgaggcgag cacctccttg atttcattca gcgactttcg cccgaggttc ggcgtcttca
    24361 ggagctcggt ttcgctgcgc tggatcaggt cgccgatgta gtagatgttc tcggccttca
    24421 ggcagttggc cgagcgtacg gtgagctcca ggtcgtccac cggacgcagc aggatcgggt
    24481 cgacggtcgg cgacttgggc gcctcggcgg gcagcgcggt gccctccagc tgcgcgaaca
    24541 ccgacagctg gtcgacgagg acgcgtgccg cgtagcggat cgcctcctcg ggctcgatcg
    24601 agccgttggt ctcgatatcc atgatcagct tgtcgaggtc ggtgcgctgc tcgacgcgcg
    24661 ccgactccac cgcgtaggac acgcggcgca ccgggctgaa cgaggcgtcg aggatgatgc
    24721 ggccgatgcc tttggtttct tcgggcagga agcgcatgtt gcccggcacg tagccgcggc
    24781 cggactcgac cttgatctgc atctcgagct tgccggtggc cgatagatga gcgaccacgt
    24841 gctccggatt gacgatctcc acgtcgtgcg agggctctat gtcggcggca gtcaccggcc
    24901 cctcgccctt cttcttcagg ctgagaatcg cctccggccg attatgcagg cggaacacga
    24961 tgcccttcag gttgagcagg atgtcgacca cgtcctcgcc gacgccgtcc atggtcgaat
    25021 actcgtgcac cacgcccgcg atctgcactt cggtgggcgc gtagccgggc atggaggaca
    25081 gcagcacgcg ccgcagggcg ttgccgagcg tgtggccgta gccgcgctcg aacggctcca
    25141 tggtgacctt ggcgtgcgag ggcgacaggt tctgtacgtc gacgatgcgc ggcttgagaa
    25201 atccggattg cggcatgtgc gtgttttccc gttacttcga atacaactcg atgatcaggc
    25261 tttcgttgac cgtggacggc aggtcggtgc gcgccggcag cgacttgaag gtgcccttga
    25321 gcgccttggc gtcgacttcc agccactcgg ggaagccgcg gccttctgcg gcctcgctcg
    25381 aggccttgat gcgcagctgt tccttcgcct tgccggtcac ttggacgacg tcgcccgggc
    25441 gcacctggta cgacgggata ttgacgcgcc tgccgttcac ctggatgccg ttgtggcgca
    25501 ccacctggcg cgcctcggtg cgcgaggcgc cgaagcccat gcggtaggcg acgttgtcga
    25561 gacgcgtctc gagcagctgc agcagccgct cgccggtgac gcccttggag cgcgaggctt
    25621 ccgcgtaggt cctgcggaac tggcgctcga gcacgccgta ggtgcggcgc agcttctgct
    25681 tctcgcgcag ctgcttgccg aagtcggaca tgcggctgcc gctcttctgc ccgtgctggc
    25741 cgggcgcgta gccgcgccgc tcgaccgcgc agcggtcggt gaagcacttc tcgcccttga
    25801 ggaagagctt ctcgccctcc ctgcggcatt ggcggcactt ggcgtcgaga tctctggcca
    25861 tgcgttctcc ttgcctatcc tgtcagacgc gccggcgctt gggcgcgcgg cagccgttgt
    25921 gcggaaccgg agtgacgtcc gcgatgctgc cgatcttcag gccgatcgcg ttcaacgcgc
    25981 gcaccgcgga ttcgcggccg ggccccgggc ccttgatgcg cacttcgatg ttcttcacgc
    26041 cgcactcctg cgcggcgcgc ccggcgcgct ccgcggcgac ctgcgccgcg aacggcgtcg
    26101 acttgcgcga gcccttgaag ccggcgttgc ccgaggtggc ccaggcaagc gcgttgccct
    26161 ggcggtcggt gatggtgacg atggtgttgt tgaacgacgc gtggatgtgc gcgatgcctt
    26221 cggcaacgtt cttcttggcc ttcttgcgcg cgcgcgccga cgctgtttgc tgcagttgct
    26281 gctgggtggg ggtagtcctg ctggccatgg tcgcggtccc gggttacgcg gccttgccgg
    26341 ccggcacgtt caacttgatg gcggccttgc gcggcccctt gcgcgtgcgc gcattggtgc
    26401 gcgtgcgctg cccgcgcacc ggcaggccct tgcggtggcg ctggcctcgg tagcagccca
    26461 ggtccatcaa ccgcttgatc gacatcgaga cttcgcgccg caggtcgccc tcgagcgtga
    26521 ggcgcgtcac ctgctcgcgc aggcgctcca tctccgcatc gctcaggtcc ttgatcttgc
    26581 ggctgcgctc gacgccggcg gcggcgcaca gcgcctgcgc gcgcgcgcgg ccgatgccga
    26641 aaatcgccgt cagcgcgatc tccgcgtgct ggtggttcgg aatattgatg cctgcaatgc
    26701 gtgccatggg ggtcccggga aacctggaat tatacctgtt tcgtcaaggc gttatcaacc
    26761 ctgccgctgc ttgtggcgcg ggtccgagca gatgacgcgc accacgcgct ttcgccgcac
    26821 gatcttgcac ttgcggcaga tcttcttcac cgatgccatt actttcatct ggctgctcct
    26881 tgtctctcgc ggcgcctagc gaaccggcag gccgcccttg aaattcgcct tgcgcagcag
    26941 gctttcgtac tggtgcgtca tgacgtaggc ctgcacctgc gacatgaagt ccatcgtcac
    27001 caccacgatg atcagcagcg acgtaccgcc gaagtaaaaa ggcacgttcc accgcaggat
    27061 gaggaattcg ggcagcaggc agaccagcgt cacgtagatc gcgccagcga gcgtcaggcg
    27121 cgtcaggatc ttctccaggt agcgcgaggt ctgctcgccc gggcggatgc ccggcacgaa
    27181 ggcgccggac ttcttcaggt tgtccgccgt ttccttcggg ttgtactgca gcgccgtgta
    27241 gaagaagcaa aagaagatga tgagccccgc gtacatcagc acgtagatcg gctggccggg
    27301 ggtgagcgtc gcagcgatgt cgcgcagcca gaccatgccc tcggccgcgc cgaaccagcc
    27361 gagcagcgtc gccgggaaca ggatcagcga cgaggcgaag atcggcggga tgacccccgc
    27421 catgttgagc ttgaacggca ggtgcgagct ctggccgccg tagacgcggt tgcccacttg
    27481 gcgcttggcg tagttgacca ggatcttcct ctggccgcgc tcgacgaaca ccaccaccgc
    27541 cgtgaccagc agcaccgcgg cggcgatcag cagtgccgtc agcgggtgca tggcgccggt
    27601 gcgcaccagt tccagcgttc ccgcgatcgc gcttggcagc ccggccgcga tgcccgcgaa
    27661 gatgattaag gagatgccat tgcccaggcc gcgttcggtg atctgctcgc cgagccacat
    27721 caggaacata gtgcctgtca ccagggtggt caccgtggtg acgcggaaca tgaggcccgg
    27781 gtcgagcacc agtccggcct gcgattcgag cgcaaccgaa atgcccgtcg cctggaacaa
    27841 cgccaggaat accgtgccgt agcgcgtgta ctgggtgatc ttgcgccgcc ccgactcgcc
    27901 ttccttgcgc agctggtcga ggtgcgggct caccgcggtc atcagctgca tgatgatcga
    27961 ggccgagatg taaggcatga tgcccagggc gaagatggtg aagcgcgaca gcgcgccgcc
    28021 cgagaacatg ttgaacatgc ccaggatgcc gccctgctgg ctcttgaaca ggtcggcgag
    28081 cacgttcggg tcgattcccg gcaccgggat gtgcgcgccg atgcggaaca cgaccagggc
    28141 gcccagcagg aacagcaggc gccgcttcag gtcgccgtag cgcccggcct tgcccgcgga
    28201 tggcagcgtg gtcgccaagc cctattcccc ggccggctgc gccgcgggcg cggcgtcctc
    28261 gaccttgccg cccgcggcct cgatcgccgc gcgcgcgcct ttgctcgcct tgatgccctt
    28321 cagcgtgacg ctgcgcgtca gctttccgga caggatgact ttcgccatgc gcgcttcgcg
    28381 cggcacgatg ccctgcgcct tgagcgcgcc gaggtcgatg tcgccggcta tcatgctctc
    28441 cagttccgaa agccggacct ccgcggtatg cgtgcgcgtg ggcgaggcga agccgcgctt
    28501 cggcaggcgc cggtgcagcg gcatctggcc gccctcgaag ccgaccttgt ggtagccgcc
    28561 ggagcgcgcg cgctggccct tgtggccgcg gccggcggtc ttgccccagc ccgagccgac
    28621 gccgcggccg acacggtggc gcgacttctt cgcgccgggc gccggtttga gtgagttcaa
    28681 gcgcatctgt ctatccttcc acccgcacga ggtaagagac cttctcgatc atgccgcgca
    28741 ccgacggcgt gtccacgagc tccaccgtct ggcggatcct gcgcaggccg aggccgcgca
    28801 ccgtggcgcg atggtccgcg tcgcagccgg ccgtgctctt caccagggtt accttgatct
    28861 tgccgttcgc catggccgcc cctaggaggt aacttcctcg accgtcttgc cgcgcttggc
    28921 cgcgatctcg gcgggcgtgt tcatcgcctg cagtccctgc agcgtcgcgc gcaccacgtt
    28981 gtacgggttg gtcgagccga agcatttggc cagcacgttg gtgatgccca tcacctcgaa
    29041 taccgcgcgc atcgggccgc cggcgatgat gccggtaccc tcgtgcgccg gctgcatcag
    29101 cacggtggcc gcgccatggt ggccgatcac ggcgtggtgc agcgtaccgt tcttgagctt
    29161 caccttgaag agcttcctgc gcgcctcttc catcgctttt tgcacagcca ccggcacctc
    29221 gcgcgccttg cccttgccca tgccgatgcc gccgtcgccg tcgccgacga cggtaagcgc
    29281 ggcgaagccg agcacccggc cgcccttcac caccttggtg acgcggttca ccgacaccat
    29341 cttctcgcgc aggccgtcgg tgcgctcctc gctctgctgc attctgggct tcatttttgc
    29401 catggcatgt ccttagaact tgagtccgcc ggcgcgagcc gcttcggcaa gcgccttgac
    29461 gcgaccgtga tagcggtagc cggcgcgatc gaacgcgacc gcctcgatgc cggcctgctt
    29521 ggccttctcg gcgatgcgcg cccccaccgt ctcggcggcc ttgcggttgc cgccattgcc
    29581 gacctgcgcg cgcacttcct tctccagcgt cgaggccgac gccagcacct tgtcgccgct
    29641 gctcgaggtg agctgggcgt agatgtggct gttggtccgg tgcaccgtga gccgcaccgc
    29701 accgatctcg cggatcttga ggcgcgtctg gcgcgcgcgc cgcactcggg cttcgttctt
    29761 gtctcgcatg tcgggctccg ctacttcttc ttcgtctctt taaggacgac gacctcgttg
    29821 gcatagcgca cgcccttgcc cttgtagggc tcgggcggct tggtatcgcg gatctccgcc
    29881 gcgatctggc cgacgagctg cttgtcgatg ccgctcacca cgacctcggt ctgcgtcggc
    29941 gtggccacct tcacgccctt gggcatctgg tgcacgaccg ggtgcgagaa gccgactgac
    30001 agattgagct tgtcgccctg cgcctgtgcg cggtagccca cgccgaccag cgcgagcttc
    30061 ttctcgaagc ccttggtcac gccggtcacc atgttggcca ccagcgcgcg cagcgtgccg
    30121 gacatcgcgg tggcatggtg cgagttgccg agcgccttgc ataccagcgt ctcgccttcc
    30181 ttctgtatgc ccacgttcgg atccatcggc cgcgccatgg tgccgagcgg acccttcacc
    30241 gagatggtgc ccgaggcgag cgtcacctcc acgcccttgg gcagcgcaat cgggtacttg
    30301 gcgattcggg acatggctct ctccttacgc cacgatgcac agcacttcgc cgcccacgcc
    30361 gtctgcgcgc gccttgcggt ccgtcatcac gccgcgcgag gtcgacacga tcgccacgcc
    30421 caggccgttc atgacccggg ggatatcgtc gcggcccttg tacacgcgca gcccgggctt
    30481 ggagacgcgc tcgaggcgct cgatcaccgg ccggccggcg tagtacttga ggccgatgcg
    30541 cagctgcttc atgggctggt cttctcccac gacgaagtcc tcgatgtagc cctcgtcctt
    30601 cagcaccttg gcgatggaga ccttgagctt ggagcagggc atgctgacct ccgcgtgccc
    30661 caccatctgg gcgttgcgga tgcgggtcag catgtcggcg atcggatcgg tcatgctcat
    30721 gcgcgggtct ccttaccagc tcgccttgat cacgcccggc acctcgccgc gcagcgccag
    30781 gtcgcgcagc ttgttgcgtc ccaggccgaa cttgcggtac acgccgcgcg ggcgcccggt
    30841 gatcgcgcag cggttgcgca gccgcgtcgg cgatgcgtcg cgcggcagct tctgcagctt
    30901 ctcgcgcgcg gcttcgcggt cctcgtcctg cgccctggga tcgcgaatca ggtcgagcag
    30961 cgcctggcgc cgggccttga acttggccac ggtctggcgg cgcttctgtt cgcggagttt
    31021 gacggcgagt tttgccatgt gcggatcctc agtgcttgaa cgggaagcgg aacgcagcga
    31081 gcagtgcctt cgcctcgtcg tccgtcttcg ccgtcgtggt aatggcgatg ttcatgccgc
    31141 ggagtgcgtc gatcttgtcg tattcgatct ccgggaagat gatctgctcg cgcacgc
//
LOCUS       DBNHKE010000179         8968 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1353374, whole genome shotgun sequence.
ACCESSION   DBNHKE010000179 DBNHKE010000000
VERSION     DBNHKE010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1353374"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /gene="ppsA"
                     /locus_tag="AC16O9_12445"
     CDS             <1..1654
                     /gene="ppsA"
                     /locus_tag="AC16O9_12445"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR6517463.1"
                     /translation="PAIVRRSLGTKQQKMVYAETAAAGRSVRTVEVPEAERNRFSLAD
                     ADVLELARAALAIEKHYRRPMDIEWAKDGVDGHIYVLQARPETVKSRAHVGTEESFRL
                     KQRGKVLATGRAIGHKIGAGPVRLVAAAALMSKVKKGDVLVTDMTDPNWEPVMKLAAA
                     IVTNRGGRTCHAAIIARELGIPAVVGCGDATETLREGQPVTVSCAEGDTGNVYEGSLP
                     FEVSAVERGAMPAIGVKIAMNVGNPQLAFDFAQLPNAGVGLARLEFVINNEIGVHPRA
                     CLEHAQLPAELRAKVSAASRGYSDPVAFFSEKLAEGVATIAAAFWPKPVIVRLSDFKS
                     NEYRKLLGGERYEPEEENPMLGFRGAARYLAESYRACFDLECAALRKVRDELGLTNVE
                     LMVPFVRTVDEARRVVALLAENGLRRGENGLRIIMMCELPSNALLAEAFLAHFDGFSI
                     GSNDLTQLTLGLDRDSGLVAEEFDERNAAVKALLAMAIRACRKHNKYVGICGQGPSDH
                     PDLARWLMEQGIDSLSLNPDTVVATWRALAEPAPAPQPSAAA"
     gene            complement(1640..2083)
                     /gene="smpB"
                     /locus_tag="AC16O9_12450"
     CDS             complement(1640..2083)
                     /gene="smpB"
                     /locus_tag="AC16O9_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6517464.1"
                     /translation="MSIVQNRKAFHDYFIEERYEAGIALEGWEVKAIRAGRAQLADAY
                     VLIKGEELWLIGGNITPLATVSTHYVPDPARTRKLLLHAEEIRRLIGKVQQRGYALIP
                     LDLHFNKGRIKLELGLARGKLKHDKRAAERDKQWQRDKQKLLRSR"
     gene            2116..2559
                     /locus_tag="AC16O9_12455"
     CDS             2116..2559
                     /locus_tag="AC16O9_12455"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR6517465.1"
                     /translation="MKRIARSAIVEHAAPAMYALVEDVAAYPQFLPWCRAAHVRERSA
                     QRTVATLAVGLKGLRYEFTTQNDSRPGEAIELTLLRGPFRHFAAHWRFTPLGESAARI
                     DFAMEYEFAGALVSRALGPLFETIADTMVDAFKRRADAVYGQAAR"
     gene            2540..2803
                     /locus_tag="AC16O9_12460"
     CDS             2540..2803
                     /locus_tag="AC16O9_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR6517466.1"
                     /translation="MGRLRVELIAARPGRAARVRLDLEEGATVRAALAAAGWNAAARG
                     VGIYGRRVSLDTRLADGDRVEIYRALSADPKEARRRRAKPRRR"
     gene            complement(2810..3307)
                     /locus_tag="AC16O9_12465"
     CDS             complement(2810..3307)
                     /locus_tag="AC16O9_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6517467.1"
                     /translation="MTKAMILAVMLAFAASAFAQMYRWVDKDGRVHYTATPPPAGVKA
                     RTLRAPVTPPPAADDAARDDAAKDAGAKDARKGPLTPAEQEQEFRKRQLEAQKAREKQ
                     EMAAKEAETKRENCARAREALATFDSGQRISRTNAQGERYYLDDDARARETEAARRAV
                     QDWCG"
     gene            3391..4851
                     /gene="guaB"
                     /locus_tag="AC16O9_12470"
     CDS             3391..4851
                     /gene="guaB"
                     /locus_tag="AC16O9_12470"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6517468.1"
                     /translation="MRVIQKALTFDDVLLLPAHSRVLPREVSLKTRLTRQLELNIPIV
                     SAAMDTVTEARLAIAIAQEGGLGVIHKNFTPQQQAAEVAKVKRFESGVLRDPMTIAPE
                     LSVREAMALQKQHRISGFPVVKGDKVVGIVTNRDLRFETRLDQPVKNIMTPQKSLITV
                     REGASRAQALELLHKHRLERVLVVDADWRLLGLVTVKDIMKETEHPNACKDAQGKLRV
                     GAAVGVGEGTEERVAALVEAGADVLVVDTAHGHAQGVLDRVQWIKKHFPTMQVIGGNI
                     GTADGARALVDHGADGVKVGIGPGSICTTRIVAGVGVPQITAIQDVAAALAKTDVPLI
                     ADGGVRYSGDAAKALGAGAHAVMLGSLFAGTDESPGEIELYQGRSYKSYRGMGSLGAM
                     QKGAADRYFQDSEMNVDKLVPEGVEGRVPYKGSVVWVLQQLTGGMRASMGYTGCATIE
                     ELRTRSKFVEITTAGMRESHVHDVQIVKEAPNYRAD"
     gene            4862..6424
                     /gene="guaA"
                     /locus_tag="AC16O9_12475"
     CDS             4862..6424
                     /gene="guaA"
                     /locus_tag="AC16O9_12475"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6517469.1"
                     /translation="MRDKILILDFGAQYTQLIARRVREARVYCEIHPCDVDNAFVRAF
                     APRGVILSGSHMSAYEESTARAPQSVFELGVPVLGICYGMQTMAQQLGGKVEAGKVRE
                     FGFAEVRARGHTKLLEGIEDSKNAQGHGLLRVWMSHGDKVTDLPGGFKLMASTDACPV
                     AAMADEQRRFYGVQFHPEVTHTPQGARILQRFVLDICGCAGDWSMPDYVSEAVARIRN
                     QVGKDEVILGLSGGVDSSVAAALIHKAIGDQLTCVFVDHGLLRLNEAAQVMETFARHM
                     RLKVIHVDAAADFYGALAGVPDPEAKRKIIGRHFVEVFQREAAKVKNARWLAQGTIYP
                     DVIESAGAKTKKAQSIKSHHNVGGLPESLHLKLLEPLRELFKDEVRELGLALGLPRDM
                     VFRHPFPGPGLGVRILGEVKREYADLLRRADDIFIQELRAADWYDKTAQAFAVFVPVK
                     SVGVMGDGRTYEYVIALRAVQTTDFMTADWAPLPHELLARVSSRIINEVRGINRVTYD
                     ISSKPPATIEWE"
     gene            6583..7656
                     /locus_tag="AC16O9_12480"
     CDS             6583..7656
                     /locus_tag="AC16O9_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6517470.1"
                     /translation="MKRNTTLKLMAAGVAVALAAGSGSALAQKRVKWKMQSAFGSTLP
                     HLGTSGPRFTKNVSEATDGRFDIKFFEPGALVPALECFDAASKGSVESCWTTPGYHTG
                     KIGPGVAFFTAVPFGPTFGEFMAWKRHGGGDKVRDEIYGEHGLMAIDCFAIGPETSGW
                     FKNRVTSLDQLKGMKMRFFGLGAQVMQKLGVSTQLLAAADIYPALERGVIDATEFSMP
                     SIDQKLGFHQIAKNNYFPGWHQQVSVSELLMNKKAYEALPASYKKVLELGLGESVMAT
                     YAETEWRNPAAMNENVEKHGVSNVRWTDEQLASFEKAWFEVMGELSAKDATFKKIADS
                     YIAFRKQYKVWGEAQQLNPTYLK"
     gene            7764..>8968
                     /locus_tag="AC16O9_12485"
     CDS             7764..>8968
                     /locus_tag="AC16O9_12485"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6517471.1"
                     /translation="MELLLRIGSRIFNRYVIVIGLLVLLGAGYAYVSSLTGEAQEEFL
                     YFVHEYLPIVMFLTLAFLLFSGFPVAFILGGLAMLFGLIGYFLGIFKLIEFFNFMPRI
                     WGQAAENLVLVAIPAFVFMGVMMERSGIANDLLYCVQVLLKRVPGALALAVTIMGTIL
                     AAMTGIIGASVTMMTALALPTMIRQGYNHGLACGTIAASGTLGILIPPSIMLIIMADL
                     LSVSAGDVFSAAIIPGLALATLYIAYVIFVAWVKPAMAPPLPPDMLTVPKGKMLGLLG
                     RAFFPPVFLIGMIKGSILFGWATPSEAGAVGAFGAMLLALFNGRLTLAVLEGVCHTSA
                     RTVAMIFFIIISATCFAYVYRSLGGDDIVEHLIKSSGLTDWGFLILIMSITFVLGFFL
                     DWIEITLIVL"
BASE COUNT         1467 a         2929 c         3064 g         1508 t
ORIGIN      
        1 gccggcaatc gtgcgccgct cgctcggcac caagcagcag aagatggtct acgccgagac
       61 cgcggccgcc ggccgctcgg tgcgcacggt ggaggtgccg gaggccgagc gcaaccgttt
      121 ctcgctcgcg gacgccgatg tgctcgagct ggcgcgggcc gcgctggcga tcgagaagca
      181 ctatcgccga cccatggaca tcgagtgggc gaaggacggc gtggacgggc acatctacgt
      241 gctgcaggca cgaccggaga cggtgaagtc gcgcgcgcac gtcggtaccg aggagagctt
      301 ccgcctgaag cagcgcggca aggtgctggc cacggggcgc gccatcggcc acaagatcgg
      361 cgccgggccg gtgcgcctgg tggccgcagc cgcgctgatg tcgaaggtga aaaagggcga
      421 cgtgctggtc accgacatga ccgacccgaa ctgggagccg gtgatgaagc tcgccgccgc
      481 catcgtcacc aaccgcggcg ggcgcacctg ccacgcggcg atcatcgcgc gcgagctcgg
      541 catcccggcg gtggtgggct gcggcgacgc caccgagacg ctgcgcgaag ggcagccggt
      601 cacggtctca tgtgcggaag gcgacaccgg caacgtctac gagggcagcc tgccgttcga
      661 ggtcagcgcg gtggagcgcg gtgcgatgcc cgcgatcggc gtcaagatcg ccatgaacgt
      721 cggcaatccg cagctggcat tcgatttcgc ccagctgccg aacgcgggcg tcgggctggc
      781 gcgcctggag ttcgtgatca acaacgaaat cggcgtgcat ccgcgcgcct gcctcgagca
      841 cgcgcagctg cccgccgagc tgcgcgccaa ggtcagcgcc gcgagccgcg gttactccga
      901 ccccgtggcc ttcttcagcg agaagctggc cgagggcgtg gcgaccatcg cggcggcatt
      961 ctggcccaag ccggtgatcg tgcgcctctc ggacttcaag tccaacgaat accgcaagct
     1021 cctcggcggc gagcgctacg agcccgagga agagaacccg atgctcgggt tccggggcgc
     1081 cgcgcgctac ctcgccgaaa gctaccgcgc ctgcttcgac ctggaatgcg ccgcgctgcg
     1141 caaggtgcgc gacgagctcg gcctgaccaa cgtcgagctg atggtgccgt tcgtgcggac
     1201 cgtcgatgaa gcccggcgcg tggtcgcgct gctggcggaa aacggcctgc gccgcggcga
     1261 gaacgggctg aggatcatca tgatgtgcga gctgccgtcg aatgcgctgc tggccgaggc
     1321 gttcctggcc cacttcgacg gcttttccat cggctccaac gacctgacgc agctgaccct
     1381 cggcctggac cgcgattccg gcctggtcgc cgaggaattc gacgagcgaa acgccgccgt
     1441 caaggcgctg ctcgcgatgg cgatccgcgc ctgccgcaag cacaacaagt acgtcggcat
     1501 ctgcggccag ggaccgtcag accaccccga cctcgcgcgc tggctgatgg agcagggcat
     1561 cgacagcctg tcgctcaacc cggatacggt ggtcgccacc tggcgcgcac tggcagagcc
     1621 ggcgcccgcg ccgcagccct cagcggctgc gtagcagctt ctgcttgtcg cgctgccatt
     1681 gcttgtcgcg ctcggcggcg cgcttgtcgt gcttcagctt gccgcgcgcc aggccgagct
     1741 cgagcttgat gcggcccttg ttgaagtgca agtcgagcgg gatcagcgcg tagccgcgct
     1801 gctgcacctt gccgatcagg cggcggatct cctcggcatg cagcaggagc ttgcgcgtgc
     1861 gcgccgggtc gggcacgtag tgcgtggaaa ccgtcgccag cggcgtgatg ttgccgccga
     1921 tcagccacag ctcctcgccc ttgatcagca cgtaggcgtc ggcgagctga gcgcggccgg
     1981 cgcggatcgc cttcacctcc cagccctcga gagcgatgcc ggcctcgtaa cgctcctcga
     2041 tgaagtagtc gtgaaaggcc ttgcggttct ggacgatcga catgggcgcg tgcggcgggc
     2101 tgacgcgatt ctcccatgaa gcgcatcgcg cgctccgcga tcgtcgagca cgcggcgccg
     2161 gccatgtacg cgctggtcga ggacgtcgcc gcctacccgc agttcctgcc ctggtgccgc
     2221 gcggcgcacg ttcgcgagcg cagcgcgcag cgcacggtgg ccacgctcgc cgtgggcctg
     2281 aagggcctgc gctacgagtt caccacgcag aacgacagcc ggccgggcga ggcgatcgag
     2341 ctgacgctgc tgcggggtcc gtttcgccac tttgcggcgc actggcgctt cacccccctc
     2401 ggtgagagcg cggcgcgcat cgatttcgcc atggagtacg agtttgccgg ggcactcgtg
     2461 tcccgcgcgc tcggccctct gttcgaaacc atcgccgaca ccatggtcga cgccttcaag
     2521 cggcgcgccg acgcggtcta tgggcaggct gcgcgttgag ctgatcgccg cgcgtccggg
     2581 gcgcgcggcc cgcgtacggc tcgatctgga ggagggcgcc accgtacgcg cggccctcgc
     2641 ggccgcgggg tggaacgccg ccgcgcgcgg cgtcggcatc tacgggcgcc gggtgagcct
     2701 ggatacgcgg ctcgccgacg gcgatcgcgt ggagatctac cgggcgctca gcgcggaccc
     2761 gaaagaggcg cgccgccggc gcgcgaagcc gcgccggcgc tgagcgggct cagccgcacc
     2821 agtcctgcac cgcccggcgc gcagcttccg tttcgcgcgc gcgcgcgtcg tcgtccaaat
     2881 agtagcgctc gccctgggcg ttggtgcgcg agatgcgctg gccgctgtcg aaggtggcaa
     2941 gcgcttcccg ggcgcgcgcg cagttctccc gcttggtctc ggcttccttt gccgccattt
     3001 cctgcttttc gcgcgccttc tgcgcctcga gctggcgctt gcggaattcc tgctcctgtt
     3061 ccgccggcgt gagcggaccc ttgcgcgcgt ccttggcgcc ggcgtccttg gcggcgtcgt
     3121 ccctggcggc gtcatccgcg gccggcggcg gcgtcacggg cgcccgcagg gtgcgcgcct
     3181 tcacgcccgc gggtggcggc gtcgccgtgt agtgcacgcg gccgtccttg tcgacccagc
     3241 ggtacatctg cgcgaacgcg ctcgccgcaa acgcgagcat cactgccaag atcatggctt
     3301 tcgtcatgcg ctcatcctct ccggctgccg ccgagccgcg atcggcgttg cagacgcgag
     3361 tataatgccg ctttgcatga aaggctgagc atgcgcgtca tccagaaggc gctgaccttc
     3421 gacgatgtcc ttctcctccc cgcgcattcg cgggtcctac cgcgcgaagt ttccctcaag
     3481 acccgcctca cccggcagct cgagctcaac attcccatcg tctcggcggc gatggatacg
     3541 gtgaccgagg cacgcctggc gatcgccatc gctcaggaag gcggcctggg cgtgatccac
     3601 aagaatttca cgcctcagca gcaggcggcc gaggtggcga aggtcaagcg cttcgagtcg
     3661 ggcgtgttgc gcgacccgat gaccatcgcg ccggagctgt cggtgcgcga ggccatggcg
     3721 ctgcagaagc agcatcgcat ctccggtttc cctgtggtca agggcgacaa ggtggtgggc
     3781 atcgtcacca accgcgacct gcgcttcgag acccggctcg accagccggt gaagaacatc
     3841 atgacgccgc agaagagcct gatcacggtg cgcgaagggg caagccgcgc gcaagcgctc
     3901 gaactgctgc acaagcaccg cctcgagcgc gtgctggtcg tcgacgcgga ctggcgcctg
     3961 ctcggcctgg tgacggtcaa ggacatcatg aaggaaaccg agcacccgaa cgcgtgcaag
     4021 gacgcgcagg gcaagctgcg cgtgggcgcc gcggtcggcg tgggcgaggg caccgaggag
     4081 cgcgtcgcgg cgctggtgga ggcgggcgcc gacgtgctgg tggtggacac ggcgcacggc
     4141 cacgcgcagg gcgtgctcga ccgcgtgcag tggatcaaga agcactttcc gacgatgcag
     4201 gtgatcggcg gcaacatcgg caccgcggac ggggcgcgcg cgctcgtcga ccacggcgcc
     4261 gacggcgtca aggtcggcat cggcccgggc tcgatctgca ccacacgcat cgtcgcgggc
     4321 gtcggggtgc cgcagatcac cgccatccag gatgtcgccg cggcgctcgc caagaccgac
     4381 gtgccgctca tcgccgatgg cggcgtgcgc tactcgggcg acgcggccaa ggcgctcggc
     4441 gccggcgcgc acgccgtgat gctgggctcg ctgttcgcgg gcaccgacga gtcgcccggc
     4501 gagatcgagc tctaccaggg ccgctcctac aagtcctacc gcggcatggg gtcgctgggc
     4561 gccatgcaga agggcgccgc cgatcgctac ttccaggatt ccgagatgaa cgtggacaag
     4621 ctggtgcccg agggcgtcga ggggcgcgtg ccgtacaagg gcagtgtcgt ctgggtcctg
     4681 cagcagctca ccggcggcat gcgtgccagc atgggctaca ccggatgcgc aaccatcgaa
     4741 gagctccgca cccgatcgaa gttcgtggaa atcacgacgg cgggcatgcg cgaatcgcac
     4801 gtgcacgacg tgcagatcgt caaggaagcg cccaactacc gcgccgactg aagaatcggg
     4861 gatgcgcgac aagatcctga tcctcgattt cggggcgcag tacacgcagc tcatcgcgcg
     4921 gcgcgtgcgc gaggcccgcg tgtactgcga gatccaccct tgcgatgtcg acaatgcgtt
     4981 cgtgcgcgcg tttgcgccgc gcggcgtgat cctttccggc agccacatgt cggcctacga
     5041 ggagagcacc gcacgggcgc cgcaatcggt gttcgagctg ggggtgccgg tgctcggcat
     5101 ctgctatggc atgcagacca tggcgcagca gctcggcggc aaggtcgagg ccggcaaggt
     5161 gcgcgagttc ggcttcgccg aagtgcgggc ccgggggcac accaaactgc tcgagggcat
     5221 cgaggattcg aaaaacgcgc agggccacgg cttgctgcgc gtgtggatga gccacggcga
     5281 caaggtgacc gatctgcctg gcgggttcaa gctcatggcc tcgaccgacg cctgcccggt
     5341 ggccgccatg gccgacgagc agcgtcgttt ctacggcgtg caattccatc ctgaggtcac
     5401 ccacaccccc cagggtgcac gtatcctgca acgcttcgtg ctcgacatct gcgggtgcgc
     5461 cggcgactgg agcatgccgg actacgtctc ggaggcggtg gcccggatcc gcaaccaagt
     5521 cggcaaggac gaggtgatcc tcggcctttc gggcggcgtc gactcctcgg tcgccgcggc
     5581 cctgatccac aaggcaatcg gcgaccagct cacctgcgta ttcgtcgatc acgggctgct
     5641 gcgcctcaac gaggcggcgc aggtcatgga gaccttcgcc cgccacatgc gcctgaaggt
     5701 catccacgtc gacgcggctg cggatttcta cggcgccctt gccggggtgc cggatcccga
     5761 ggccaagcgc aagatcatcg gtcggcattt cgtcgaagtg ttccagcgcg aggcggcgaa
     5821 agtgaagaac gcaaggtggc tcgcgcaggg cacgatctac ccggacgtca ttgaatcggc
     5881 gggcgcgaag acgaagaagg cgcagagcat caagtcgcac cacaatgtcg gcgggctccc
     5941 ggaatcgctg cacttgaagc tgctcgagcc gctgcgcgag ctgttcaagg acgaggtgcg
     6001 cgagctcggg ctggcgctcg gcctgccgcg cgacatggtg ttccggcacc cgtttcccgg
     6061 gccggggctg ggcgtgcgca tcctcggcga ggtgaagcgc gagtatgccg acctgctgag
     6121 acgcgcggac gacatcttca tccaggagct gcgcgccgcg gactggtacg acaagacggc
     6181 gcaggcgttc gccgtgttcg tgccggtgaa gtcggtcggt gtgatgggcg acgggcgcac
     6241 ctacgagtac gtgatcgcac tgcgcgctgt gcagaccacg gatttcatga cggccgattg
     6301 ggcgccgctg ccgcacgaat tgctggcgcg agtctccagc cgcatcatca acgaagtgcg
     6361 cggcatcaac cgcgtcacct acgacatttc ctccaagccg ccggccacta tcgagtggga
     6421 gtgattggtg cggcgcacca tgcgttacgg ggttggaatt catccgcgag tccactatca
     6481 tggattgatc cgcgtcaata atgcggagaa gtagcgggcc aggctcggcg ttgggtgcgt
     6541 tcgagaccgg tcaaatcaaa gcccaacacg gaggagaacc gcatgaagcg aaatacaacg
     6601 cttaagctca tggctgcagg ggtggccgtc gcgctggctg caggatcggg cagcgcgctc
     6661 gcgcagaagc gcgtgaaatg gaagatgcag agcgcctttg gcagcacgct gccgcacctg
     6721 ggtacatctg gccctcgctt cacgaaaaac gtcagtgaag cgacagacgg acggtttgac
     6781 atcaagttct tcgagccggg cgcgctggtg ccggcgctcg aatgcttcga cgcagcttcc
     6841 aagggctcgg tcgagtcatg ctggacgacc cccggctatc acaccggcaa gatcggacct
     6901 ggggtcgcgt tcttcaccgc ggtgccgttc ggaccgactt tcggcgaatt tatggcgtgg
     6961 aagcgccacg gtggcggcga caaggtgcgc gacgagattt atggcgagca cggcctgatg
     7021 gcgatcgact gtttcgccat cggccccgag acctccggct ggttcaagaa ccgggtcact
     7081 tcgctcgacc agctgaaggg catgaagatg cgcttcttcg gactgggcgc gcaagtgatg
     7141 cagaagctcg gcgtgtcgac gcagttgctg gcggccgccg acatctaccc ggcgctggag
     7201 cgcggcgtga tcgacgccac cgagttctcg atgccctcga tcgaccagaa gctcggcttc
     7261 caccagattg cgaagaacaa ctacttcccc ggctggcacc agcaagtctc ggtctccgag
     7321 ctgctgatga acaagaaggc ctacgaggcg ctgccggcgt cttacaagaa ggtcctcgag
     7381 ctgggattgg gtgaatcggt catggcgacc tacgccgaaa ccgagtggcg caatcccgcg
     7441 gcaatgaacg agaacgtcga aaagcacggg gtcagcaacg tccgctggac cgacgagcaa
     7501 ctcgctagct tcgaaaaagc atggttcgag gtaatggggg agctctcggc caaggatgcg
     7561 acctttaaga agatcgcgga tagctatatc gcgtttcgca agcaatacaa ggtctggggc
     7621 gaggcgcaac agctcaaccc gacgtatctg aagtaggcga gacggcgcgc gcggcgcgcg
     7681 tggccatttt gaccggaaag ccggggcgcc cgttggcgcc ccggttgttt ctttgcccgg
     7741 cctacgggaa tccaggtgac tgaatggaac tgttgctaag aatcggcagc cgtatcttca
     7801 accggtacgt gatcgttatc ggcctgctgg tgctgctggg cgccggctac gcctacgtgt
     7861 cgagcctcac cggcgaggcg caggaagagt tcctgtattt cgtgcacgag tacctgccga
     7921 tcgtcatgtt cctgacgctc gcgttcctgc tgttctcagg cttcccggtg gccttcattc
     7981 tcggcggcct ggcgatgctg ttcgggttga tcggctattt cctcggcatc ttcaagctga
     8041 tcgagttttt caatttcatg ccccgcatct gggggcaagc ggccgagaac ctggtgctgg
     8101 tggcgatccc ggcgttcgtc ttcatgggcg tgatgatgga gcgcagcggc atcgccaacg
     8161 acctgctgta ctgcgtgcag gtgttgctca agcgcgtacc cggcgcgctg gcgctggcgg
     8221 tcaccatcat gggcacgatt ctcgcagcta tgaccggcat tatcggcgcc tcggtgacga
     8281 tgatgaccgc gctcgcgctg ccgaccatga tccgacaggg ctacaaccac ggcctggctt
     8341 gcggcaccat cgccgcgtcg ggcacgctcg gcatcctgat cccgccgagc atcatgctga
     8401 tcatcatggc cgacctgttg tcggtgtcgg cgggcgacgt gttcagtgcc gcgatcattc
     8461 cgggcctggc gctggcaacg ctctacattg cctacgtcat atttgttgcc tgggtcaagc
     8521 cggcaatggc gccgccgctg cccccggaca tgctgacggt accgaagggc aagatgctcg
     8581 ggctgctcgg ccgcgcgttc ttcccgccgg tgttcctgat cggcatgatc aaaggctcga
     8641 ttcttttcgg ttgggccacc ccgagcgagg ccggcgcggt cggcgccttc ggcgcgatgc
     8701 tgctggcgtt gttcaatggc cgccttacgc tcgccgtgct cgagggcgta tgtcacacgt
     8761 cagcacgcac ggtggcgatg atcttcttca tcatcatcag cgccacctgc tttgcctatg
     8821 tctaccgctc gctgggcggc gacgacatcg tcgagcactt gatcaagagc tccggtctta
     8881 cagactgggg tttcctgatc ctgatcatgt cgatcacttt cgtgctcggt ttcttcctcg
     8941 actggatcga gatcacgctg atcgtgct
//
LOCUS       DBNHKE010000180        11949 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1088524, whole genome shotgun sequence.
ACCESSION   DBNHKE010000180 DBNHKE010000000
VERSION     DBNHKE010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1088524"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4062)
                     /gene="rpoC"
                     /locus_tag="AC16O9_12490"
     CDS             complement(<1..4062)
                     /gene="rpoC"
                     /locus_tag="AC16O9_12490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR6517472.1"
                     /translation="MKALLELFKQVQQDEDFEAIRIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERM
                     GHIELASPTAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDPGLTPLKRCQLL
                     TEDDYLAKVEEFGDDFSAAMGAEGIRALLRAIDINRDIDQLRKDLEATSSDAKIKKLA
                     KRLKVLEGFKASNIKPDWMIMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLELLGLAT
                     TIKAAKRLVEQEVPEVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDIVL
                     GLYYATRERIGAQGEGMSFADLAEVSRAYESRNVGLNAKAWVRIREQDLTPEGEKVER
                     IKRYETTVGRALLSEILPAGLSFELLNKPLKKKEISRLINVGFRRCGLRETVIFADKL
                     MQAGFTMATRGGISFGVHDMRIPTAKQELIAAAETEVKEIESQYTSGLVTVGERYNKV
                     VDIWSRAGEQVGKVMMEQLGSEEVTDRHGKKVRQEAFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVTQDLVVTEDDCGTQNGIAMRALVEGGEVVEALRERILGRVLAVDLINPETQDT
                     LYTAGSLVDEEAIDTIDNVGIDEVKVRTPLTCDTRWGVCAKCYGRDLGRGSVVNVGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVQSQVEAKSAGTVRFSPGMRYVSNAKG
                     DKVVISRSGEVTIVDDNNRERERHKVPYGATLQVVDGDAIRAGRIVAAWDPHHRPIVT
                     EYAGTVKFEHVEEGVTVAKQIDDVTGLSTLVVIDHKRRGSATAKGQRPSVRLINEAGD
                     DVRIAGTDHAVNISFPVGAVIAVRDNQQVLVGEVLARIPQETSKTRDITGGLPRVAEL
                     FEARAPKDKAILAEYTGTVSFGKDTKGKQRLVITDLDGTQHEELIPKDKHVLVHDGQV
                     VNRGEMIVDGPVDPHDLLRLLGVEALARYIIDEVQDVYRLQGVKINDKHIEVIVRQML
                     RRVQIEDPGETGFIQGEQVERAELLEENEKAEKEGKKTATFSYMLLGITKASLSTDSF
                     ISAASFQETTRVLTEAAIMGKRDGLRGLKENVIV"
     gene            complement(4062..8348)
                     /gene="rpoB"
                     /locus_tag="AC16O9_12495"
     CDS             complement(4062..8348)
                     /gene="rpoB"
                     /locus_tag="AC16O9_12495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR6517473.1"
                     /translation="MTYSLTERKRIRKSFAKRSTVQTVPFLIATQLESYTAFLQAHMA
                     PERRRNEGLQAAFTSIFPITSHSGNARLEFVSFQLGEPPFDVKECQQRGLTYASPLRA
                     KVRLVILDKEAPKPTTKEVKEQEVYMGEIPLMTTTGSFIINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDVVYFRVDRRRKMPVTILLKALG
                     FTPELILKEFFDFDGFHLEPNHVLFEVVPERLRGEVARFEITDKSGKTIVAKDKRITV
                     KHIRDMEAAGIKRIVAPDEFLLGRTLAHNVVSAETGEILASANEELTETLLAKLREAK
                     VGKIQTLFTNDLDQGPYVSQTLRTDETVDVNAARVAIYRMMRPGEPPTEDAVESLFNG
                     LFFAEERYDLSAVGRMKFNRRVGVPCETSWQIRLKSVALSKESEEEVRAYFKHAPELS
                     LGKVSIEGVLTEAEAQQVVDKMYQDLKAKGVDRQKLEAKLEQRYTLSPRDIVEVIRIL
                     VELRNGRGDIDDIDHLGNRRVRSVGELAENQFRAGLVRVERAVKERLSQAESDNLMPH
                     DLINAKPISAAIKEFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVR
                     DVHPTHYGRVCPIETPEGPNIGLINSLALYARTNKYGFLETPYRKVTNSKVTNEIVYL
                     SAIEEGNYVIAQANAAIDKAGKLSDPLVSCRNRNEFMLSTPDRVEYMDVAPSQIVSVA
                     ASLIPFLEHDDANRALMGSNMQRQAVPCLRPEKPLVGTGVERTAAIDSGTCVVALRGG
                     IVDYVDANRVVVRVNDEETVPGDVGVDIYKLTKYTRSNQNTNINQRPIVKQGERLAKG
                     DVVADGASTDLGELALGQNMLVAFMPWNGYNFEDSILISERVVSDDRFTSIHIEELTV
                     VARDTKLGPEEITRDISNLSEAQLSRLDESGIVYIGAEVEAGDVLVGKVTPKGETQLT
                     PEEKLLRAIFGEKASDVKDTSLRVPSGISGCVIDVQVFTREGIERDKRSSQIIEDDLR
                     RYKTDLADQMRIVESDTFERLERLLTGKTANGGPKKLAKGAKITKGYLDTLERFDWFD
                     IRLANEEAAAQLEGLKESLAQKRREFDAMFEAKRKKLTQGDELPPGVLKMVKVYVAVK
                     RRLQPGDKMAGRHGNKGVISKIVPVEDMPHMADGTTLDIVLNPLGVPSRMNVGQILET
                     HLGWAAKGLGLKLGEMIKAQAKIAEVRKTVERIYNASGRDEDLGKLSDEEVMQLAQNL
                     REGVPFATPVFDGASEAEINAMLELAGLPVSGQVTLFDGRTGEAFDRPVTVGYMHVLK
                     LHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLT
                     VKSDDITGRTKVYENIVKGDHKIDAGMPESFNVLVKDIRSLGIDIDLEKY"
     gene            complement(8471..8848)
                     /gene="rplL"
                     /locus_tag="AC16O9_12500"
     CDS             complement(8471..8848)
                     /gene="rplL"
                     /locus_tag="AC16O9_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR6517474.1"
                     /translation="MERAQILEAISSMSVLELSQLIKDVEEKFGVSAAAAVAVAAPAA
                     GGAAAGAAAEEKTEFTVVLAATGENKVNVIKAVREVTSLGLKEAKDLVDGAPKPVKEG
                     VSKAEADAVAKKLTDAGAKVEIK"
     gene            complement(8880..9410)
                     /gene="rplJ"
                     /locus_tag="AC16O9_12505"
     CDS             complement(8880..9410)
                     /gene="rplJ"
                     /locus_tag="AC16O9_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR6517475.1"
                     /translation="MSLSLEQKQAVVSEIATQLAKAQAVIVAENRGLSVEAVTSLRAK
                     ARKSGLYLRVLKNTLARRAVKGTPFESLSGELFGPLVYGIAQDPVSGAKVLAEFAKEN
                     ELFVIKGGAMSNAKMSSTDVKALATMPSREELLAKFAGTLQAPIAKLARTLNEVPGKF
                     ARALAAVHAAREKSAA"
     gene            complement(9611..10309)
                     /gene="rplA"
                     /locus_tag="AC16O9_12510"
     CDS             complement(9611..10309)
                     /gene="rplA"
                     /locus_tag="AC16O9_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR6517476.1"
                     /translation="MAAISKRRKVIVGKADRNKAYAVIDAMKLIKETATAKFDEAVDV
                     AVNLGIDTSKSDQTVRGAVVLPAGTGKTVRIAVFAQGDKAQAAKAAGADVVGMDDLAE
                     SVKAGKLDFDVVIAEPAAMRVVGQLGQILGPRGLMPNPKVGTVTPDVAAAVKNAKAGQ
                     VQFRADKTGIVQCTIGRASFTPEQLRDNLAALVDALNKSKPQHTKGVFLRKLSVSSTM
                     GIGVRVDPATLQAQ"
     gene            complement(10310..10741)
                     /gene="rplK"
                     /locus_tag="AC16O9_12515"
     CDS             complement(10310..10741)
                     /gene="rplK"
                     /locus_tag="AC16O9_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR6517477.1"
                     /translation="MAKKVVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKVEPGLPLPVVITAYADKSFSFIIKTPPTTVLIKKAAGIESGSPRPHTDKVGKIT
                     RKQVEEIAKAKTPDLTAADLDAAMRTVAGSARSMGILVEGM"
     gene            complement(10798..11358)
                     /gene="nusG"
                     /locus_tag="AC16O9_12520"
     CDS             complement(10798..11358)
                     /gene="nusG"
                     /locus_tag="AC16O9_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR6517478.1"
                     /translation="MEKNLKQTEAGKRWYVVHAYSGFEKSVQRALEDRIARAGMQDKF
                     GKILVPVEEVVEMKGGQKHMSERKFFPGYVLVEMEMDDDTWHLVKNTNKVTGFVGGSA
                     MRPTPISEKEVNAIMQQMREGVEKPKPKVLFENGEMVRVKDGPFTDFQGTVEDVNYEK
                     SRLRVAVTIFGRSTPVELEFGQVEKA"
     gene            complement(11343..11702)
                     /gene="secE"
                     /locus_tag="AC16O9_12525"
     CDS             complement(11343..11702)
                     /gene="secE"
                     /locus_tag="AC16O9_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR6517479.1"
                     /translation="MAATDIFKLVIAAAMVAGGIVGYYWLGDAALVLRVLAVVAGIGA
                     GAAVAGFSAPGREFVVFAQEAIVEVKKVVWPSRKETVQTTAAVFAFVVVMALFLWVSD
                     KTLEWVLYDLILGWKKT"
     gene            complement(11712..11787)
                     /locus_tag="AC16O9_12530"
     tRNA            complement(11712..11787)
                     /locus_tag="AC16O9_12530"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11752..11754),aa:Trp,seq:cca)
     gene            complement(11807..>11949)
                     /locus_tag="AC16O9_12535"
     CDS             complement(11807..>11949)
                     /locus_tag="AC16O9_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR6517480.1"
                     /translation="EMVMPGDNIKMVVTLITPVAMEQGLRFAIREGGKTVGAGVVAKV
                     IE"
BASE COUNT         1832 a         4038 c         3688 g         2391 t
ORIGIN      
        1 gacgatcacg ttttccttca ggccgcgcag gccgtcacgc ttgcccatga tcgcggcttc
       61 ggtgagcacg cgcgtcgttt cctggaacga tgccgccgag atgaacgagt cggtcgaaag
      121 cgacgccttg gtgatgccga gcagcatgta ggaaaacgta gcggtcttct tgccttcctt
      181 ctccgccttc tcgttctcct cgagcagctc ggcgcgttcc acctgctcac cctggatgaa
      241 gcccgtctcg cccggatcct cgatctgcac gcggcgcagc atctggcgca cgatgacctc
      301 gatgtgcttg tcgttgatct tcacgccctg caggcggtac acgtcctgca cttcatcgat
      361 gatatagcgc gcaagcgctt cgacgccgag caggcgcagc aggtcgtgcg gatccaccgg
      421 cccgtcgacg atcatctcgc ccctgttcac cacctggccg tcgtgtacca gcacgtgctt
      481 gtccttcgga atcagctcct cgtgctgcgt accgtcgagg tcggtgatca ccaggcgctg
      541 cttgcccttg gtgtccttgc cgaaggacac ggtacccgtg tactcggcga ggatcgcctt
      601 gtccttgggc gcgcgcgcct cgaacagctc cgccacccgc ggcaggccac cggtgatgtc
      661 gcgcgtcttg gacgtttcct gcggaatgcg ggcgagcacc tcgcccacca gcacctgctg
      721 gttgtcgcgc accgcgatca ccgcgcccac cgggaagctg atgttcacgg catggtcggt
      781 gccggcgatg cgtacatcgt cgcctgcttc gttgatcagc cggaccgacg gacgctggcc
      841 cttggccgtg gcgctgccgc gacgcttgtg gtcgatgacc accagcgtcg agaggccggt
      901 cacgtcatcg atctgcttcg ccaccgtcac gccttcctcg acgtgctcga acttcacggt
      961 gccggcgtac tcggtgacga tgggacggtg gtgcggatcc caggccgcga cgatcctgcc
     1021 ggcgcggatg gcatcgccat ccaccacctg cagcgtcgcc ccgtagggca ccttgtggcg
     1081 ctcgcgctcc cggttgttgt cgtcgacgat cgtcacttcg cccgagcgcg agatcaccac
     1141 cttgtcgccc ttggcgttcg agacgtagcg catgcccgga gagaagcgca cggtgccggc
     1201 cgacttcgcc tccacctggc tctgcaccgc agtacggctg gccgcgccgc cgatgtggaa
     1261 cgtgcgcatg gtgagctgcg tgcccggctc gccgatcgac tgcgcggcga tcacgccgat
     1321 tgcctcgccg acgttgacca ccgagccccg gcccaggtcc cggccgtagc acttcgcgca
     1381 cacgccccag cgcgtatcgc aggtcagggg tgtgcgcacc ttgacttcat cgatgccgac
     1441 attgtcgatg gtgtcgattg cctcctcgtc caccagcgag cccgccgtat acagggtgtc
     1501 ctgcgtctcc gggttgatca ggtcgacggc cagcacacgg ccgaggatgc gctcgcgcag
     1561 cgcctcgacc acttcgccgc cctcgaccag cgcgcgcatg gcgatgccat tttgcgtgcc
     1621 gcagtcgtcc tccgtcacca ccaggtcctg cgtcacgtcc accaggcgac gcgtcaggta
     1681 gcccgagttc gccgtcttca gcgcggtgtc ggcgagaccc ttgcgcgcgc cgtgcgtgga
     1741 aatgaagtac tgcagcacgt tcaggccttc gcggaaattg gccgtgatcg gcgtttcgat
     1801 gatcgagccg tcgggcttgg ccatcaggcc gcgcatgcct gcgagctggc ggatctgggc
     1861 ggcggagccg cgtgcgcccg agtcggccat catgtagatc gaattaaacg cttcctggcg
     1921 caccttcttg ccgtggcggt cggtcacttc ctcgctgccg agctgctcca tcatcacctt
     1981 gccgacctgc tcaccggccc gcgaccagat atcgaccacc ttgttgtagc gctcgcccac
     2041 cgtgaccagg ccggaggtgt actgcgattc gatctccttc acctcggtct cggccgccgc
     2101 gatgagctcc tgcttggcgg tcgggatgcg catgtcgtgc acgccgaacg aaatgccgcc
     2161 gcgcgtggcc atggtgaagc cggcctgcat cagcttgtcg gcgaagatca cggtctcgcg
     2221 cagcccgcag cgccggaagc cgacgttgat gaggcgcgag atctccttct tcttgagcgg
     2281 cttgttgagc agctcgaagg aaaggcccgc cggcaggatc tcggaaagca gcgcccggcc
     2341 caccgtggtc tcgtagcgct tgatgcgctc gaccttctcg ccttccggcg tcaggtcctg
     2401 ctcgcgaatg cgaacccagg ccttggcgtt caggccgacg ttgcgcgatt cgtaggcgcg
     2461 cgagacttcg gcgagatcgg caaagctcat gccctcgccc tgcgcgccga tgcgctcgcg
     2521 cgtggcgtag tacaggccga gcacgatgtc ctgcgagggc acgatgatcg gctcgccgtt
     2581 ggcgggcgaa agaacgttgt tcgaggcgag catcagcgtg cgcgcttcca tctgcgcttc
     2641 cagcgacagc ggcacgtgca ccgccatctg gtcaccgtcg aagtcggcgt tgaatgccgc
     2701 gcacacgagc gggtgcagct ggatcgcctt gccctcgatc agcaccggct cgaacgcctg
     2761 gatgccgagg cggtgcaggg tcggggcgcg gttcagcatc accgggtgct cgcggatcac
     2821 ctcttcaagg atgtcccaca cctccggcac ttcctgctcc accaggcgct tggcggcctt
     2881 gatggtggtg gcgagcccga ggagctcgag cttgttgaag atgaacggct tgaagagctc
     2941 gagggccatc ttcttcggca agccgcactg gtgcagcttc aactgcgggc cgaccacgat
     3001 gaccgagcgc cccgagtagt cgacgcgctt gccgagcagg ttctggcgga agcgtccgcc
     3061 cttgcccttg atcatgtcgg cgagcgattt gagcggtcgc ttgttggcgc cggtcatcgc
     3121 cttgccgcgg cgaccgttgt cgaggagcga gtccacggcc tcctgcagca tgcgcttttc
     3181 attgcgcacg atgatctcgg gcgccttgag ctcgagcagg cgcttcaggc ggttgttgcg
     3241 gttgatgacg cgccgataca ggtcgttcag gtccgaagtg gcgaagcgcc cgccatcgag
     3301 cggcaccagg ggccgcagct cgggcggcag caccggcagc acttccatga tcatccagtc
     3361 gggcttgatg ttggaggcct tgaagccctc gagcactttg aggcgcttgg cgagcttctt
     3421 gatcttggcg tccgacgagg tcgcctcgag gtccttgcgc agctggtcga tgtcgcggtt
     3481 gatgtcgatg gcccgcagca gcgcgcggat gccttcggca cccatcgccg ccgagaagtc
     3541 gtcgccgaat tcctcgacct tggcgaggta gtcgtcctcg gtcagcagct ggcagcgctt
     3601 gagcggggtg aggcccgggt cggtgacgac ataggcctcg aagtacagca cgcgctcgat
     3661 gtcgcgcagc gtcatgtcga gcaccaggcc caggcgcgac ggcaggctct tcaggaacca
     3721 gatgtgcgcc gtggggctgg cgagctcgat gtggcccatg cgctcgcggc gcaccttggc
     3781 gagcgtcacc tccacgccgc acttctcgca gatcacgccg cggtgcttca ggcgcttgta
     3841 cttgccgcac aggcactcgt agtccttgat cggcccgaag atcttggcgc agaacaagcc
     3901 atcgcgctcc ggcttgaaag tgcggtagtt gatggtctcc ggcttcttca cttcgccgta
     3961 ggaccacgag cggatcttct cgggcgacgc gagcccgatg cggatggctt cgaagtcctc
     4021 gtcctgctgg acctgcttga agagctcaag tagcgctttc atcagtactt ctccaggtcg
     4081 atatcgatgc ccagcgagcg gatgtccttc accagcacgt tgaacgactc gggcatgccg
     4141 gcgtcgatct tgtggtcgcc cttgacgatg ttctcgtaca ccttcgtgcg cccggtgatg
     4201 tcgtcggatt tcaccgtcag catctcctgc agcgtgtagg cagcgccgta ggcctccagc
     4261 gcccacacct ccatctcgcc gaagcgctgc ccgccgaact gtgccttgcc gccgagcggc
     4321 tgctgcgtaa cgaggctgta cggcccggtc gagcgtgcat gcatcttgtc gtccaccagg
     4381 tggtggagct tgagcacgtg catgtagccc accgtgaccg ggcgatcgaa ggcttcgccg
     4441 gtgcgcccgt cgaacaacgt cacctggccc gaaaccggca aaccggccag ctccagcatg
     4501 gcgttgatct ccgcttcgga cgcgccgtcg aacaccgggg tggcgaaggg cacgccctcc
     4561 ctgaggttct gcgccagctg catcacttcc tcatcgctga gcttgcccag gtcttcgtcc
     4621 ctgccgctcg cgttgtagat gcgctccacg gtcttgcgga cctcggcgat cttcgcctgc
     4681 gccttgatca tctcgccgag cttcaggccg aggcctttcg ccgcccagcc caagtgcgtc
     4741 tcgaggatct ggccgacgtt catgcgcgaa ggcacgccca gcgggttgag cacgatgtcg
     4801 agcgtcgtgc cgtccgccat gtgcggcatg tcctccaccg gcacgatctt ggagatcacg
     4861 cccttgttgc cgtggcgccc cgccatcttg tcgccgggct gcaggcgccg cttcaccgcc
     4921 acgtacacct tcaccatctt cagcacgccc ggcggcagct cgtcgccttg cgtgagcttc
     4981 ttgcgcttgg cctcgaacat ggcgtcgaac tcgcgccgct tctgtgccag cgattccttc
     5041 agaccttcga gctgcgccgc cgcttcctcg ttggccagcc gaatatcgaa ccagtcgaag
     5101 cgctcgagcg tatcgaggta gcccttcgtg atcttggcgc ccttggcgag cttcttcggc
     5161 ccgccgttgg cggtcttgcc ggtaagcaac cgctccaggc gctcgaaggt gtccgattcg
     5221 acgatccgca tctggtcggc gaggtcggtc ttgtagcggc gcaggtcgtc ttcgatgatc
     5281 tgcgagctgc gcttgtcgcg ctcgatgcct tcccgcgtga acacctgcac gtcgatcacg
     5341 cagcccgaga tgcccgaggg cacgcgcagc gacgtgtcct tcacgtccga ggccttctcg
     5401 ccgaagatcg cgcgcagcag cttctcctcc ggcgtaagct gggtctcgcc cttcggcgtc
     5461 accttgccca cgagcacgtc gcccgcctcg acttcagcgc cgatgtagac gatgcccgat
     5521 tcgtcgagcc gcgagagctg cgcttcagac aggttcgaga tgtcgcgcgt aatctcctcg
     5581 ggcccgagct tggtgtcgcg cgccaccacc gtcagctcct cgatgtggat cgaggtgaag
     5641 cgatcgtccg ataccacacg ctcggagatg aggatcgaat cctcgaagtt gtagccgttc
     5701 cacggcatga acgcgaccag catgttctgg ccgagcgcga gctcgccaag gtcggtcgag
     5761 gcgccgtcgg cgacgacgtc gcccttggcg agccgctcgc cctgcttcac gatggggcgc
     5821 tggttgatgt tggtgttctg gttggagcgc gtgtacttcg tcagcttgta gatgtccacg
     5881 ccgacgtcgc ccggcaccgt ttcctcgtcg ttcacgcgca ccaccacgcg gttggcgtcg
     5941 acgtagtcga cgatgccgcc gcgcagtgcc accacgcagg tgcccgaatc gatcgccgcc
     6001 gtgcgctcga cgccggttcc gaccaggggt ttttcgggac gcaggcaggg caccgcctgg
     6061 cgttgcatgt tggagcccat caacgcgcgg ttggcatcgt cgtgctcgag aaacgggatc
     6121 agcgaggcgg ccaccgacac gatctggctc ggcgccacgt ccatgtattc cacccggtcc
     6181 ggcgtggaca gcatgaattc gtttcggttg cggcacgaca ccagcgggtc gctcagcttg
     6241 cccgccttgt cgatcgcggc attggcctgg gcgatgacgt agttgccttc ctcgatcgcc
     6301 gacaggtaga cgatctcgtt ggtcaccttg ctgttcgtca ccttccgata cggggtctcc
     6361 aggaagccgt acttgttggt gcgcgcatac agcgccagcg agttgatgag gccgatgttg
     6421 gggccttccg gcgtttcgat cgggcacacg cggccgtagt gcgtcgggtg cacgtcccgc
     6481 acctcgaagc ccgcacgctc gcgcgtcagt ccgcccggtc ccagcgcgga gacgcgccgc
     6541 ttgtgcgtga tctccgaaag cgggttggtc tgatccatga actgcgacag ctggctcgag
     6601 ccgaagaact ccttgatcgc cgccgagatc ggcttggcat tgatcaggtc gtgcggcatc
     6661 aggttgtcgc tttcggcctg cgacaggcgc tccttcaccg cacgctcgac ccgcaccagg
     6721 ccggcgcgga actggttctc ggcgagctcg cccaccgagc gcacgcgccg gttgccgagg
     6781 tgatcgatgt cgtcgatgtc gccgcggccg ttgcgcagct cgacgaggat ccggatcact
     6841 tccacgatgt cacgcgggct gagcgtgtag cgctgctcca gcttcgcctc aagcttctgc
     6901 cggtccacgc ccttggcctt gagatcctgg tacatcttgt cgacgacttg ctgcgcctcg
     6961 gcctcggtca gcacaccctc gatggagacc ttgccgaggg acagctcggg cgcatgcttg
     7021 aagtacgcgc ggacttcctc ttcggattcc ttggaaagcg cgacgctctt cagccggatc
     7081 tgccagctcg tttcgcaggg cacaccgacg cggcggttga atttcatgcg gccgaccgcg
     7141 gacaggtcgt agcgctcctc tgcgaaaaac aggccgttga acaacgactc caccgcatct
     7201 tccgtcggcg gttcgccggg tcgcatcatg cggtagatgg ccacgcgggc cgcgttgacg
     7261 tcgaccgttt cgtcggtgcg cagggtctgc gacacgtacg gcccctggtc gagatcgttc
     7321 gtgaacagcg tctggatctt gccgactttc gcttcgcgca gcttggccag cagcgtctcg
     7381 gtgagctcct cgttggcgct tgccaggatc tcgccggtct ccgcgctcac cacgttgtgc
     7441 gcgagcgtgc gtccgagcag aaactcgtcg ggcgccacga tcctcttgat gcctgcagct
     7501 tccatgtccc ggatgtgctt gaccgtgatg cgcttgtcct tggcgacgat ggtcttgccg
     7561 gacttgtcgg tgatctcgaa gcgcgccact tcgccgcgca ggcgctcggg gaccacctcg
     7621 aacagcacgt ggttcggctc gaggtgaaaa ccgtcgaaat cgaagaattc cttcaggatc
     7681 agctccggcg tgaacccaag cgccttcagc aggatcgtca ccggcatctt gcggcgacgg
     7741 tcgacgcgga aatacaccac gtccttcggg tcgaattcga agtcaagcca ggagccgcgg
     7801 tagggaatga cgcgcgccga aaacagcagc ttgcccgagc tgtgcgtctt gccgcggtcg
     7861 tgctcgaaaa acacgccggg ggagcgatgc agctgcgaga cgatgacgcg ctcggtgccg
     7921 ttgatgatga acgagccggt ggtcgtcatg agcgggatct cgcccatgta cacctcctgc
     7981 tccttcactt ccttggtggt cggcttgggc gcctccttgt ccaggatcac gagacgcacc
     8041 ttcgcgcgca gcgggctggc gtaggtgagg ccgcgctgct ggcattcctt gacgtcgaag
     8101 ggcggctcgc cgagctggaa gctgacgaac tcgaggcgcg cgtttcccga atggctggtg
     8161 atcgggaaga tcgaggtgaa cgccgcctgc agcccctcgt tcctgcgccg ctcgggcgcc
     8221 atgtgcgcct gcaggaacgc ggtataggac tcgagctgcg tcgcgatcag gaacggcacg
     8281 gtctgcaccg tggaacgctt ggcgaagctc ttgcggatgc gtttacgctc ggtcaacgaa
     8341 taggtcatga aggctcctgg ccgcaagttc ctgaaatgac aaggaaaaag ccctcggctg
     8401 cgagggcgct ttccttggcc ggtttccggg gagggtcgct atgacccacc ccggattccg
     8461 aagttcctgc ttacttgatc tccacctttg cgccggcgtc ggtgagcttc ttggcgacgg
     8521 cgtccgcctc cgccttggag accccctcct tgaccggctt cggcgcgccg tcgaccaggt
     8581 ccttggcctc cttgaggccg aggctggtca cctcccgcac cgccttgatc acgttcacct
     8641 tgttctcgcc ggtggcggcg agcacgaccg tgaactcggt cttctcttcc gctgcggcgc
     8701 ccgcggcggc cccgcccgcg gccggggccg cgaccgccac tgccgcagcg gccgacaccc
     8761 cgaacttctc ttccacgtcc ttgatgagct gcgagagctc gaggacgctc atgctcgaaa
     8821 tggcttccag aatctgtgcg cgttccatct tctatctcct gattcgaatg ggttcctgat
     8881 taagcggccg atttttccct cgcggcgtgc acggcggcga gcgcccgggc aaacttgccc
     8941 ggaacctcgt tgagtgtgcg cgcaagcttc gcgatggggg cttgcagcgt gccggcaaat
     9001 ttcgccagga gctcttcgcg gcttggcatc gtggccagcg ccttgacgtc cgtagaggac
     9061 atcttggcgt tggacatggc cccgcccttg atgacgaaaa gctcgttttc cttggcgaat
     9121 tccgccagca ccttggcccc cgacacggga tcctgcgcga tgccgtaaac cagcgggccg
     9181 aacagctccc cgctcagcga ctcgaacggc gtgcccttga cggcgcgccg cgcgagcgtg
     9241 ttcttcagca cgcgcaggta gaggccggat ttccgcgcct tcgcgcgcag gctggtcacg
     9301 gcttcgacgc tcaggccgcg gttctccgcg acgatgaccg cctgagcctt cgccagctgc
     9361 gttgcgatct cggaaacgac cgcctgcttc tgctccagac tgagactcaa ggtctacctc
     9421 ctagcgttga tcaaacggag gcccgtttcc gaacccccac cctggcgacc ttgaatcagg
     9481 aaccgcacat gcagtcctgc ttcgggtacg ccgtctgcgt agggcacatg ccggtttaag
     9541 cccatcgggc ccctacggtc tttgacagct gccgttccga ggaacagcgg cccaaagttc
     9601 catcgtttct tcactgcgcc tgcaacgttg ccgggtcaac ccttactccg atccccatcg
     9661 tgctcgagac cgagagcttg cgcaggaaca cgcccttggt gtgctgcggc ttcgacttgt
     9721 tcagcgcgtc cacgagcgcg gcgaggttgt ctcgcaactg ctcaggagtg aacgaggcac
     9781 gaccaatcgt gcactggaca atgcccgtct tgtcggcgcg aaactgcacc tggccagcct
     9841 tggcgttctt caccgctgca gccacgtccg gcgtcactgt gcccactttc ggattcggca
     9901 tcaggccgcg cgggccgagg atctggccca gctgacccac gacgcgcatc gcagccggct
     9961 cggcgatcac cacgtcgaaa tccagctttc ccgccttcac gctctctgcc aggtcgtcca
    10021 tgccgaccac gtcggccccc gccgccttag cggcttgcgc cttgtcgcct tgcgcgaata
    10081 ccgcgatgcg caccgtcttg ccggtgcccg cgggcagcac cacggcgccg cgaaccgtct
    10141 ggtcggactt ggacgtgtcg atgccgaggt tcactgccac gtcaacggct tcgtcgaact
    10201 tggcagttgc ggtttccttg atgagcttca tggcatcaat gaccgcgtag gccttgttgc
    10261 ggtcggcctt gccgacgatg accttcctgc gcttggatat cgcggccatt tacatgccct
    10321 ccaccaggat acccatcgag cgcgcgctgc cggccaccgt gcgcatcgcg gcatcgaggt
    10381 cggccgccgt caggtccggc gtcttcgcct ttgcgatctc ctcgacttgc ttgcgggtga
    10441 tcttgcccac cttgtcggta tgcgggcgcg ggcttccgga ttcgatgccg gcggccttct
    10501 tgatcaggac ggtcgtcgga ggcgtcttga tgatgaagct gaagctcttg tccgcatacg
    10561 ccgtgatgac cacgggcaac ggcaggccgg gctccacctt ttgggtctgc gcgttgaacg
    10621 ccttgcagaa ctccatgatg ttcaggccac gctgaccgag cgcaggaccg atgggcggcg
    10681 aagggttggc cttgcccgcc ggcacctgca gcttgatgaa tcccaccact ttctttgcca
    10741 tgtctggctc ctatcgggtg caagcgaacg ccgcagcgtt ctccccgtgc aagtgactca
    10801 ggccttttcg acctggccaa actccagctc gaccggggtc gagcgtccga aaatcgtcac
    10861 cgccacgcgc aggcggctct tctcgtaatt cacgtcctcg accgtgccct ggaagtcggt
    10921 gaacggacca tccttgacgc gcaccatctc gccgttctcg aacaacacct tgggcttggg
    10981 cttctccacg ccttcgcgca tctgctgcat gatggcgttc acttccttct cggagattgg
    11041 cgtcggccgc atcgcgctgc cgccgacgaa gccggtgacc ttgttggtgt ttttcaccag
    11101 atgccaggtg tcgtcgtcca tctccatttc gacaagcacg tagccgggaa agaacttgcg
    11161 ctccgacatg tgcttctggc cgcccttcat ctccacgacc tcttccaccg gcacgagaat
    11221 cttgccgaac ttgtcctgca tgccggcgcg cgcgatgcgg tcctcgagcg cgcgctgcac
    11281 cgacttctcg aagccggagt aggcatgcac gacgtaccag cgcttgccgg cttcggtctg
    11341 ctttaggttt ttttccatcc gaggatcagg tcgtagagaa cccactcaag tgtcttgtcg
    11401 cttacccaca gaaaaagcgc catcacgacg acgaacgcga acaccgcggc cgtcgtctgc
    11461 acggtctcct tacgcgaagg ccatacgacc ttcttcactt cgactatcgc ttcctgcgca
    11521 aacacgacga attcgcgccc cggggcagag aagcccgcga cggcggcgcc agcgccgatg
    11581 cccgcgacca cggcgagcac gcgcagcacc agcgcggcgt cgcccagcca gtagtagccc
    11641 acgatgccgc ccgccaccat tgctgcggcg attacgagct tgaaaatgtc agttgcggcc
    11701 atgtcagtct ttggcagggg cagagggaat cgaaccccca accttcggtt ttggagaccg
    11761 acgctctgcc agttgagcta tgcccctatg tccgattcgc cttttcttac tcgatcactt
    11821 ttgcgacgac gccggcgccc acggtcttgc cgccctcgcg gatcgcgaag cgcaggccct
    11881 gctccatcgc caccggcgtg atcagcgtca ccaccatctt gatgttgtcc ccgggcatca
    11941 ccatctcgg
//
LOCUS       DBNHKE010000181        16175 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_930845, whole genome shotgun sequence.
ACCESSION   DBNHKE010000181 DBNHKE010000000
VERSION     DBNHKE010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16175
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_930845"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC16O9_12540"
     CDS             <1..662
                     /locus_tag="AC16O9_12540"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR6517481.1"
                     /translation="RRLGLEWNYAGPKDVWNEMRGCMDSIRGITWERLERDSSVTYPC
                     ENEGDPGQPVVFQTHFPTATGRGKFVPADLIRADERPDRDYPMVLITGRQLEHWHTGA
                     MTRRSGVLDAIEPEPMASLHPLDLAELRAKPGDVVTVESRRGRIALFARADEGTPRGA
                     VFIPFAFQEAAANLLTNPVLDPFGKIPEFKYCAVKVAKGGARPADPGYGKGRSLAQIA
                     R"
     gene            745..2415
                     /locus_tag="AC16O9_12545"
     CDS             745..2415
                     /locus_tag="AC16O9_12545"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR6517482.1"
                     /translation="MPSDIDIAQAAKLKRVSKVALERLGIAEEHLEPYGHYKAKVSLK
                     YLDSLKGKKNGKLILVTAMTPTPAGEGKTTTTVGLGDALNHIGKKAVICLREPSLGPV
                     FGMKGGAAGGGYAQVVPMEDINLHFTGDFGAIQLANNLLAAMLDNHIHHGNELGIDVR
                     RIAWKRVLDMNDRALRDVVVSLGGPGNGYPRQDGFDIVVASEVMAIFCLATSLEDLKK
                     RLGNIVVGYTREQKPVTARDLQAHGAMTVLLKDALAPNLVQTLENNPAFIHGGPFANI
                     AHGCNSVIATQSALKLADYVVTEAGFGADLGAEKFLDIKCRKSGLSPDAAVIVATIRA
                     LKYHGGVDVKEVNKENVAALEQGLVNLERHVENVSKVYGIPCVVSINRFTFDTPAEIE
                     CVQARMKKLEVPVVLATHWSDGGKGAAELAKLVVGLCEKPSQATFVYEDADPLWDKIT
                     KIAQKVYRASAVTADTKVRNQIKKLQDDGYGHYPVCVAKTQYSFSTDVAKRAAPSGHA
                     LNVREVRLAAGAEFVVMVCGDIMTMPGLPKVPSAAKIDLVDGKVVGLF"
     gene            complement(2412..3128)
                     /locus_tag="AC16O9_12550"
     CDS             complement(2412..3128)
                     /locus_tag="AC16O9_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6517483.1"
                     /translation="MELLLDPQAWIAFVTLTALELVLGIDNIVFISILVDRLPPQRRE
                     LARRIGLFLAMFMRIALLLTIAWIIGLTAPIFTVVGNEISGRDLILLAGGLFLIWKAT
                     GEIHEMYEDHATPRDPGAKAVRATFGAIIGQIIVIDLVFSLDSIITAVGMVDDVRVMV
                     AAVIASVGLMMVAAGPIGRFVSAHPTVKMLALAFLIMIGMALVADGLDFHVPKGYIYF
                     AMAFSVAVEMLNLRLRRKPR"
     gene            3185..4180
                     /locus_tag="AC16O9_12555"
     CDS             3185..4180
                     /locus_tag="AC16O9_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR6517484.1"
                     /translation="MDTLSGAIVVFLISSAFVIAAGIGLARFGDDLAEATGWGKLWVG
                     TLLVGIATSLPEVTVNVSAVWLEHNPSLALGNVFGADMINIFVIGVVALVFGVPNLFG
                     NQGRDTELLILTGLGLVALALAFGAFGDIKVLGPTSVGGLLIGALYVYGMRRVYLAGL
                     EAPGENGGEEPKGSVRNAWIGFLVSAAVVIVAGRFLASSADAIAEASGISASFIGVLL
                     VSIVTTLPEGSVTVAASLRRSYGIAMGNVYGSCAFNVTIIFIADFFHARGPLLREMQS
                     AHFAAALAAFVLMGMGYLVFKAFQSRPYAAFRALAPAIPIVYVAALYVVYVLGQA"
     gene            4313..5308
                     /locus_tag="AC16O9_12560"
     CDS             4313..5308
                     /locus_tag="AC16O9_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6517485.1"
                     /translation="MKRIFWRVLATALAVGWLGAAQAWEPSKTVEFVVPAGTGGGADQ
                     MARAIQGIVAKYKLMNESMVVINRSGGSGAEGFLDVKGSQGNPHKIIITLSNLFTTPL
                     ATGVPFNWRDLTPVKMMALDNFVLWVNAETPYKTPKAYVDAVKQAGSGKMKMGGTGAK
                     QEDQIITAAIEQLTGAKWTYIPIKGGGAVAVQLVGKHIDSSVNNPIEAVAHWRGGKLR
                     PLCVFNDNPMPYKEKLTGDLSWNSIPTCKKAGLDVSYMMLRGIFMPGGVTQDQVDYYV
                     GVLRKVQETAEWKDLMAKGAFDTTSLEGKAYADWVAREEARHVSLMKGAGFIAGQ"
     gene            5384..5890
                     /locus_tag="AC16O9_12565"
     CDS             5384..5890
                     /locus_tag="AC16O9_12565"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR6517486.1"
                     /translation="MAEQTGERPAFRFKTAELAVAGILFVLGAIVVFDSVRLGAKWAA
                     DGPQPGYFPFYLGCLICAASLINIVQALRITEEKNGSFVGRGQLKLVLSVLIPSAVFA
                     GLIGWLGIYVSAILYVGFFMRWLGRYAWWKLLLVAFGNSTVFYLIFEVWFKIPLPKGP
                     LEALLGLD"
     gene            5904..7412
                     /locus_tag="AC16O9_12570"
     CDS             5904..7412
                     /locus_tag="AC16O9_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR6517487.1"
                     /translation="MEEIRSLMQGFAIALSFTNLALMFVGIVLGVIIGVLPGLGGANG
                     VAILLPLTFAMAQTPEGQTSAIILLSCIYWGALFGGAITSILFNIPGEPWSVATTFDG
                     YPLSQKGRAGHALTAAFTSSFIGAFIAVIVITFLSPIIAGFALEFGPAEFFSVYLLTF
                     CAFVGMSKEPPFKTVAAMMLGFALAAVGTDPVTGTLRLTFGSIHLLTGFDFLIAVIGL
                     FGIGEILLTLEEGLAFKGASAKLNLRAVIETWKELPRYWLTSLRSSAVGCFMGIVPGG
                     ATPASFMSYGVARRFSKDGDKFGTGQIEGVVAPETAAHAAGTAALLPMITLGVPGSPT
                     AAVLLGGLLIWGLQPGPLLFVEQKDFVWGLIASMYLGNIAGLIVVLTCVPLFAAILRI
                     PFSVIAPIIIVICSIGAYTVHNSMFDVWMMVVFGVAGYFFKKLAYPLAPLVLAIVLGD
                     RAEASFRQAILGSQGDMLVFFSNGLVGSITSLALFLLFWPLLSLVIRKLRGG"
     gene            complement(7409..8020)
                     /locus_tag="AC16O9_12575"
     CDS             complement(7409..8020)
                     /locus_tag="AC16O9_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6517488.1"
                     /translation="MTAFPSHPQRNSIRLQLRQNQLLKGLTAAQWGELEPLLAVADYR
                     KGEPVVRQGDEEMEQIFILEGMLKRVVSNPEGREMILRFAAETEMDTSYAAWRLRTPI
                     PYSIVAVTKVRTAELPMPQWVEFMERHTDIKLRFEHEVMKLMSEVMAHTITLHLLDAP
                     GRLSRFQRKHPELSGRIPKKELAAYLNLTPETLSRLRQKLGEP"
     gene            8109..9848
                     /gene="oxc"
                     /locus_tag="AC16O9_12580"
     CDS             8109..9848
                     /gene="oxc"
                     /locus_tag="AC16O9_12580"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HIR6517489.1"
                     /translation="MGTVEPQVAADAQAAVTDGFQLVIDALKLNGIDTIYGLPGIPIT
                     DLTRRAQADGLRVLSFRHEQNAGYAASIAGFLTQKPGICLTVSAPGFLNGLNALAHAT
                     TNCFPMILISGSSEREIVDLQQGDYEEMDQLAIAKPLAKAAFRVLHAEDIGVGVARAI
                     RAAVSGRPGGVYLDLPAKLFAQGIDAAAGKNSLIKVVDPAPRQIPAPDAVKRALDLLK
                     GANKPLIILGKGAAYAQADADIRALVEKTGIPYLPMSMAKGLLPDTHEQCASAARSFV
                     LPGADVVMLIGARLNWLLSHGKGKTWGGKSHKDWGGQKFIQVDISPTEADSNVRIDAP
                     VVGDIGSCVQALLAGVGAGWAKPPADWLNAIQEKKSTNVAKMAETLAKNPSPMNFHSA
                     LAVVRDIVKANPDAMFVNEGANALDFTRSIVDMYKPRKRIDVGTWGIMGVGMGYCVAA
                     AVVTKQQVIAIEGDSAFGFSGMEVETICRYKLPVCVVIMNNNGVYKGTDVNPRGGDVA
                     PTVFVKDARYEKLMEAFGGVGVYATTPEELRKGMEDAIRSRRPTLINAVIDETAGTES
                     GRITSLNPSAKKK"
     gene            9865..11136
                     /gene="frc"
                     /locus_tag="AC16O9_12585"
     CDS             9865..11136
                     /gene="frc"
                     /locus_tag="AC16O9_12585"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HIR6517490.1"
                     /translation="MEALKGVRILDMSHVQAGPTCSQLLAWLGADVIKFENPAGDATR
                     GQLRDVPNADSLYFTMLNCNKRSITVNMKSAEGKQVFVDLLKKCDIIMENFGPGVLDR
                     FGFTWEKIHELNPRIVMGSIKGFGSSGPYADFKAYENVAQAMGGAMSTTGTPDGPPFV
                     TGAQIGDSGTGLHLAIGLLAALRQRDLTGKGQYVEVAMMDGIMNLCRVKFRDHQRLTR
                     GSLSEYSVGTHKGMGAVPRAGNDSGGGQLGNAVRCKPGGPNDFLYIVVQEAVWSALAK
                     LIGPEVGMPDLATDPKFAKIADRRLNQNDMWEIIGEFALKHTKRELMALLNPLDVPCG
                     PVMSTEDLTHDEHVRGREMWVELDHPERGPWYNVGMPIKLSDSPAVIKRSPTLGEHTD
                     EVLKEVLGYDEGRINALKSAGAFTAPPKKAA"
     gene            11136..12056
                     /locus_tag="AC16O9_12590"
     CDS             11136..12056
                     /locus_tag="AC16O9_12590"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6517491.1"
                     /translation="MKIAIVGQQDFGKAVLEAFLARGDQVAAVFCAPEKEGARPDALR
                     AAAQEKGLKVHQFKSLKAPEAVQAMKDAGAQIGIMAFVLQFAPQEFVTLPKHGTIQYH
                     PSLLPKYRGPSSINWPISRGDKETGLTIFRPTDGLDEGAVILQKKTPVGDNDTLGNVY
                     FERLFPMGVEAMLEAADLVVAGKHKETAQDESQASYEGWFREAEAKIHWANHVDLIHN
                     LIRASDPAPGAWTTLGGVKLQLFDSRKETVRTFGAVRGKIGEVLGLEGGRLVITAQGG
                     RIHAGKVKLGDGKKLAAAEAGIQAGQILGT"
     gene            12181..14739
                     /locus_tag="AC16O9_12595"
     CDS             12181..14739
                     /locus_tag="AC16O9_12595"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HIR6517492.1"
                     /translation="MKASFWKSDWFLGLVVVVVMLFAGGSDLMQSLERKAYDIGVQSS
                     ARAPSDRIAVIAIDDTSIANIGRWPWSREVHARMTDLLAGARAKVIANTLFLFEPQLD
                     PGYQYITKLLELAARADAAAPATPAQAEMLALLRSAEAALNTDRRLAESYARAGNVLL
                     PMLFTIDIPRGKPDRPLPDFVAKNAIPAGQPELGALPAAEVYFPLENLGRAAAALGHL
                     NANPDVDGGIRTEPLVLRYFEQHFPSLSLMIAAKSLNLSPDDITIRANEGVTLGRLSI
                     GTDPALQMYTYFYKNVDGRPAFQVDSFYDVITGKIPAAKYADKIVLIGATAAGVGTTS
                     VTPVSPAMAPVLTLAHSVSSILQEHFFVAPAWAYLASWGAFLLVALYLIILLPRLSAA
                     PAALVTVLVLGVLIGVHFVLMTTQNTWMQLMVPASLLLVGHVALVTKRFIVTEAAKVK
                     SDESSAESNRMLGIAYQAQGQLDLAWDKFRQVPMSAAVMDNLYNLALDFERKRQFNKA
                     ESVFRHMHDHNPKFKDLASRLSRAKQLSETVIIGGGSTHPGGSLNLAAGEKPKFGRFV
                     VQKELGKGAMGVVYLGKDPKIGREVAIKTMALAQEFEADELAEVKERFFREAETAGRL
                     SHPNIVTIYDTGEEHDFCYIAMELLKGGDLAPYVKAPNLLPGDKVVSIVARAADALGY
                     AHKQGVVHRDVKPANMMYHPDSDTLKLTDFGIARLTDSSKTKTGMVLGTPSYMSPEQL
                     AGKRIEGRSDLFSLAVSLYQLLCGRLPFEGESMAQLMFKIASEPPTSILTVNPRVPPA
                     LVAFLDKALAKNPDARYQSGEEFAAALRAAAGAAGGARPAAPAGGPPRSVDIEL"
     gene            14744..15568
                     /locus_tag="AC16O9_12600"
     CDS             14744..15568
                     /locus_tag="AC16O9_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR6517493.1"
                     /translation="MDLSQVLEIASCTDPGMVRSHNEDSIAADPASGLVVLADGMGGY
                     NAGEVASGMATTVITTEIRQLLAKVRPYDVDPESNKIIAGQMVREQVMKANTSIYQAA
                     QSQPQYAGMGTTLVVCLFYDNKALVAHLGDSRLYRMRDGTLKQVTRDHSLLQEQIDSG
                     LITAEQAKHAQHKNLVTRALGIDPAVEPEIREYETRPGDIYLLCSDGLCDMVTDEDIS
                     MTLEALGANLKLAAQQLVQMANDNGGKDNVSVILVRVQREYPAARGVMGKFLGWLK"
     gene            15585..>16175
                     /locus_tag="AC16O9_12605"
     CDS             15585..>16175
                     /locus_tag="AC16O9_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6517494.1"
                     /translation="MAKLILSMDGLVLKEIPLSKERTTIGRKPHNDIQIDNLAVSGEH
                     AVIVTILNDCFLEDLGSTNGTLVNGNPVKKHFLQNNDVVELGKYRLKFVGEAPVPAAT
                     AERADFEKTMVLRPSAMKAAADQARAMGAGGAEAARASAAQQAAASATSMGTAERATP
                     TPTPSPTATTPMSSPAATTPTPIPALARPPAAAPAAA"
BASE COUNT         2780 a         5363 c         5487 g         2545 t
ORIGIN      
        1 cgcggcgcct ggggctcgag tggaactacg ccgggccgaa ggacgtgtgg aacgaaatgc
       61 gcggctgcat ggactcgatc cgcggcatca cctgggagcg cctggagcgc gattcctccg
      121 tcacctaccc gtgcgagaac gagggcgacc ccgggcagcc ggtcgtgttc cagacgcatt
      181 tccccaccgc caccggccgc ggcaagttcg tgcccgcgga cctgatccgc gctgacgagc
      241 gacccgaccg ggactacccg atggtgctga tcaccggacg ccagctcgag cactggcaca
      301 ccggcgccat gacgcgccgc tcgggcgtgc tcgacgccat cgagcccgag ccgatggcgt
      361 cgttgcatcc cctcgacctt gccgagctcc gggcgaagcc cggcgacgtg gtgacggtcg
      421 aatcgcggcg cggccgcatt gcgctcttcg cgcgcgccga cgagggcacc ccgcgcggcg
      481 cggtgttcat cccgttcgcc ttccaggagg ccgcggccaa tctcctcacc aacccggtgc
      541 tcgatccctt cggcaagatc ccggagttca agtactgcgc ggtgaaggtg gcgaagggcg
      601 gggcgcgccc cgccgatccc ggttacggca agggccggtc gctggcgcag atcgcccgct
      661 agggcttgac cgcagtcaag ggcgcgctcg tgcgcgctcg cctatagtcc ggcagtccgc
      721 acacatcacc ccggggaaca cgccatgccg tccgacatcg atatcgccca ggccgcaaag
      781 ctcaagcgcg tcagcaaggt cgcgctcgag cgcctgggca tcgccgagga gcacctggag
      841 ccttacgggc actacaaggc caaggtttcg ctcaagtacc tcgattcgct caaaggcaag
      901 aagaacggca agctgatcct tgtgacggcg atgacgccca ccccggcggg cgaaggcaag
      961 accacgacca cggtaggcct gggggacgcg ctcaaccaca tcggcaagaa ggcggtgatc
     1021 tgcctgcgcg agccctcgct cggcccggtg ttcggcatga agggcggcgc cgccggcggc
     1081 ggctatgcgc aggtggtgcc gatggaggac atcaacctgc atttcaccgg cgacttcggc
     1141 gccatccagc tcgccaacaa cctgctcgcg gcgatgctcg acaaccacat ccaccacggt
     1201 aacgagctcg gcatcgacgt gcgccgcatc gcctggaagc gggtgctcga catgaacgac
     1261 cgcgcgctgc gcgacgtggt ggtgtcgctg ggcgggccgg gcaacggcta cccgcgccag
     1321 gacggcttcg acatcgtcgt ggcgtccgag gtgatggcga tcttctgcct cgccacgtcc
     1381 ctggaggacc tgaagaagcg cctcggtaac atcgtcgtcg gctacacgcg cgagcaaaag
     1441 ccggtgaccg cgcgcgacct gcaggcgcac ggcgcaatga ccgtgctgct gaaggacgcg
     1501 ctggcgccga acctggtgca gacgctggag aacaaccccg cgttcatcca cggcggcccc
     1561 ttcgccaata tcgcgcacgg ctgcaactcg gtgatcgcga cgcagagcgc gctcaagctg
     1621 gccgactacg tggtcacgga ggccggcttc ggcgccgacc tcggcgcgga gaagttcctc
     1681 gacatcaagt gccgcaagag cggcctttcg cccgatgccg cggtcatcgt cgccacgatc
     1741 cgcgcgctca agtaccacgg cggcgtcgac gtgaaggaag taaacaagga gaacgtcgcc
     1801 gcgctggagc agggactggt caacctcgag cgccacgtcg agaacgtgag caaggtgtac
     1861 ggcattccct gcgtggtgtc gatcaaccgc ttcaccttcg atacgccggc cgagatcgag
     1921 tgcgtgcagg cgcgcatgaa gaagctcgag gtgccggtgg tgctcgccac gcactggagc
     1981 gacggcggca agggcgccgc ggagctcgcc aagctggtgg tcgggctatg cgagaagccc
     2041 tcccaggcga ccttcgtcta cgaggacgcc gatcccctgt gggacaagat caccaagatc
     2101 gcgcagaagg tgtaccgcgc gtccgcggtc acggccgaca ccaaggtcag gaaccagatc
     2161 aagaagctgc aggacgacgg ctacgggcac tacccggtgt gcgtggcgaa gacccagtat
     2221 tcgttctcca ccgacgtggc gaagcgcgcg gcgccgagcg gccacgcgct gaatgtgcgc
     2281 gaggtgcgtc tcgccgcggg cgcggagttc gtggtcatgg tctgcggcga catcatgacc
     2341 atgccgggac tgcccaaggt gccctcggcg gccaagatcg acctggtcga cggcaaggtg
     2401 gtggggctgt tctagcgcgg cttgcgccgc aggcgcaggt tgagcatctc gaccgccacc
     2461 gagaacgcca tcgcaaagta gatgtagccc ttgggcacat ggaaatccag gccgtcggca
     2521 acgagcgcca tgccgatcat gatgaggaag gcaagggcca gcatcttcac cgtcgggtgc
     2581 gccgacacga agcgcccgat cggcccggcc gccaccatca tcaggccgac cgaggcgatc
     2641 acggcggcga ccatcacgcg cacgtcgtcc accatgccga ccgcggtgat gatggaatcg
     2701 agcgagaaca ccaggtcgat gacgatgatc tgtccgatga tcgcgccaaa ggtggcgcga
     2761 acggctttcg cgccggggtc gcgcggggtc gcgtgatcct cgtacatctc gtggatctcg
     2821 ccggtcgctt tccagatcag gaacagcccg ccggcgagca ggatcaggtc gcggcccgag
     2881 atctcgttgc cgacgacggt gaagatcggc gcagtgaggc cgatgatcca ggcgatcgtc
     2941 agcagcagcg cgatgcgcat gaacatggcg aggaacaggc cgatgcgccg cgccagctcg
     3001 cgccgttgcg gcggcagccg gtcgaccagg atggaaatga aaacgatgtt gtcgatgccg
     3061 agcaccagct cgagcgccgt caacgtgacg aaagcgatcc aggcctgagg atcgagcagc
     3121 agttccaccc ggcaagtgta caatcgcgcg cgccccgccg gggccagaac gacaacggga
     3181 caccatggat acattgagcg gcgccatcgt cgtattcctg atcagctcgg cgttcgtcat
     3241 cgccgccggc atcggcctgg cgcgcttcgg cgacgacctc gccgaggcta ccggctgggg
     3301 caagctctgg gtggggacgc tgctggtggg tatcgccacg tcgctgcccg aggtcacggt
     3361 caacgtctcg gcggtatggc tggagcacaa cccgagcctt gcgctcggca atgtgttcgg
     3421 cgccgacatg atcaacatct tcgtcatcgg cgtggtggcg ctcgtctttg gcgtgccgaa
     3481 cctcttcggc aaccaggggc gcgatacgga gctgctgatc ctgaccggcc tcgggctggt
     3541 ggcgctcgcg ctcgctttcg gcgcgttcgg cgacatcaag gtgctcggcc cgaccagcgt
     3601 cggcgggctg ctgatcggcg cgctctacgt ctacggcatg cggcgggtgt atctcgccgg
     3661 gctggaggcg ccgggcgaga acggcggcga ggagcccaag ggcagcgtgc gcaatgcctg
     3721 gatcgggttc ctggtctctg cggccgtggt catcgtcgcg ggacgcttcc tcgcctcgag
     3781 cgccgacgcc atcgcggaag cctccggcat cagcgcgagc ttcatcggcg tgctgctggt
     3841 gtcgatcgtg acgaccctgc ccgaaggctc ggtgaccgtg gcggcgagcc tgcgccgctc
     3901 ctacggcatc gcgatgggca atgtctacgg cagctgcgcg ttcaacgtca ccatcatctt
     3961 catcgccgac ttcttccacg cgcgcggccc gctgctgcgc gaaatgcaaa gcgcgcactt
     4021 cgccgcggcg ctcgccgcgt tcgtcctgat gggcatgggc tacctggtgt tcaaggcgtt
     4081 ccagtcgcgg ccgtacgcag cgtttcgcgc gctcgcaccg gcgatcccca tcgtctacgt
     4141 tgccgcgctg tacgtggtct acgtgctggg ccaggcctga accgggccgc gcctcgattt
     4201 ttcttgacgc tggtcaatgt gggcggatta aggtaaaaca caaaatccga cacggtagtg
     4261 ccacgctcga agggtgcgcc gcggcgaccg atcaactcgg acaggaggag ttatgaagag
     4321 gatcttttgg cgcgttctcg caacagcgct tgccgtcggc tggctcggcg cggcgcaggc
     4381 gtgggaaccc agcaagaccg tggagttcgt cgtgccggct ggcaccggcg gcggcgccga
     4441 ccagatggcg cgcgcgatcc agggcatcgt tgccaagtac aagctgatga acgaatcgat
     4501 ggtggtgatc aaccgcagcg gcggctcggg cgccgagggc ttcctcgacg tcaagggctc
     4561 gcagggcaac ccgcacaaga tcatcatcac gctgtcgaac ctgttcacca cgccgctcgc
     4621 cacgggcgtg ccgttcaact ggcgtgacct gacgccggtg aagatgatgg cgctcgacaa
     4681 tttcgtgctc tgggtgaacg ccgagacgcc ctacaagacg cctaaggcgt acgtcgatgc
     4741 ggtgaagcag gccggctccg gcaagatgaa gatgggcggc acgggcgcga agcaggagga
     4801 ccagatcatc accgcagcga tcgagcagct gacgggcgcc aagtggacct acatcccgat
     4861 caagggcggc ggcgcggtgg cggtgcagct ggtgggcaag cacatcgatt cgtcggtgaa
     4921 caacccgatc gaggcggtgg cgcactggcg cgggggcaag ctccgtccgc tatgcgtgtt
     4981 caatgacaac ccgatgccgt acaaggaaaa gctgacgggc gacctgtcct ggaacagcat
     5041 tcccacctgc aagaaggcgg ggctggacgt cagctacatg atgctgcgcg gcatcttcat
     5101 gcccggcggc gtcacccagg accaggtgga ctactacgtc ggcgtgctgc gcaaggtgca
     5161 ggagacggcc gagtggaagg acctgatggc caagggtgcg ttcgatacga cctcgctgga
     5221 aggcaaggcg tatgcggact gggtggcgcg cgaggaggca cgccacgtgt cgctgatgaa
     5281 gggcgccggc ttcatcgccg gtcagtagcc gcccggattc gccgcaaggg ccgcggcgcg
     5341 cggaacgaag tccgcgcgcc gcgtttcatg tcaggaggag gaaatggcag agcagaccgg
     5401 tgagcggccg gcgttccggt tcaagaccgc cgagctggcg gtagccggaa ttcttttcgt
     5461 gctcggcgcc atcgtcgtct tcgacagcgt gcgcctgggc gcgaagtggg ccgccgacgg
     5521 gccgcagccc ggctattttc cgttctacct gggctgtctg atatgcgcgg cgtcgctgat
     5581 caacatcgtg caggcactgc gcatcacgga ggaaaagaac ggctcgttcg tcggccgcgg
     5641 gcagctgaag ttggtgctga gcgtgctcat cccctcggcc gtgtttgcgg ggctcatcgg
     5701 ctggcttggc atctacgtgt ccgcgatcct ctacgtcgga ttcttcatgc gctggctggg
     5761 tcgctacgcg tggtggaagc tgctcctggt ggcgttcggc aatagcaccg tgttctatct
     5821 gatcttcgaa gtgtggttca agattccgct gcccaagggc cccctcgagg ccctgctcgg
     5881 gctcgactga ccggagcgcg cgcgtggagg aaatccggtc gttgatgcag ggcttcgcga
     5941 ttgccctgag cttcaccaac ctcgccctga tgttcgtcgg cattgtgctg ggggtcatca
     6001 tcggcgtgct gcccggcctg gggggcgcca acggcgtcgc catcctgctg ccgcttacct
     6061 ttgccatggc gcagacgccc gagggacaga cctcggccat catcctgctg tcgtgcatct
     6121 actggggcgc cctattcggc ggcgccatca cctcgatcct gttcaatatt ccgggcgagc
     6181 cctggtcggt ggccaccacc ttcgacggct atccgctgtc gcagaagggc cgcgccggcc
     6241 acgcgctcac ggcagccttc acgtcctcgt tcatcggcgc gttcatcgcg gtgatcgtga
     6301 tcacgttcct gtcgccgatc atcgccggct tcgcgctgga gttcggcccg gcggagttct
     6361 tttcggtgta cctgctcacg ttctgcgcct tcgtcggcat gagcaaggag ccgccgttca
     6421 agacggtagc ggcgatgatg ctcggcttcg ccctcgccgc ggtcggcacc gacccggtga
     6481 ccggcacgct gcgcctgacg ttcggctcga ttcacctgct gaccggattc gacttcctga
     6541 tcgcggtgat cggcctgttc ggcatcggcg agatattgct gaccctggaa gaagggctcg
     6601 cgttcaaggg cgcctcggca aagctcaacc tgcgcgcggt gatcgagacc tggaaggagc
     6661 tgccgcgcta ctggctcacg tcgctgcgct cgtcggccgt gggctgcttc atgggcatcg
     6721 tgccgggcgg cgcgacgccg gcgtccttca tgtcgtacgg cgtcgcgcgc cgattctcga
     6781 aggacggcga caagttcggc accgggcaga tcgagggcgt ggtggcgccg gaaaccgcgg
     6841 cgcacgccgc gggcaccgcc gcgctcctgc ccatgatcac gctcggcgtg cccggctcgc
     6901 cgaccgccgc ggtgctcctc ggcggcctgc tgatctgggg cctgcagccc gggccgctgc
     6961 tgttcgtgga gcagaaggac ttcgtctggg gcctgatcgc cagcatgtac ctcggcaaca
     7021 tcgccggttt gatcgtggtg ctgacctgcg tgccgctgtt tgccgccatc ctgcgcatcc
     7081 cgttctcggt catcgcgccg atcatcatcg tcatctgctc gatcggcgcc tacacggtgc
     7141 acaactcgat gttcgacgtc tggatgatgg tggtgttcgg cgtcgcgggc tacttcttca
     7201 aaaagctcgc gtacccgctg gcgccgctgg tgctcgccat cgtgctcggc gaccgcgccg
     7261 aggcctcgtt ccggcaggcg atcctcggct cgcagggcga catgctggtg ttcttctcca
     7321 acggcctggt cggctcgatc accagcctgg cgctcttcct gctgttctgg ccgttgctct
     7381 cgctcgtaat ccgcaagctg cgcggcggct agggctcgcc gagcttctgc cgcaggcgcg
     7441 acagcgtctc cggcgtgaga ttcaggtagg ccgcgagctc cttcttcggg atgcgcccgg
     7501 agagctcggg gtgcttcctc tggaagcgcg acagccgccc gggcgcatcg agcaggtgca
     7561 gggtgatggt gtgcgccatc acctcgctca tcagcttcat cacctcgtgc tcgaagcgca
     7621 gcttgatgtc ggtgtggcgc tccatgaatt ccacccattg cggcatcggc agctctgcgg
     7681 tgcgcacctt ggtcaccgcg acgatggagt aggggatcgg tgtgcgcagg cgccacgccg
     7741 cgtaggacgt gtccatctcg gtctccgcgg cgaagcgcag gatcatctcg cggccttccg
     7801 ggttggacac cacgcgcttc agcatcccct cgaggatgaa gatctgctcc atctcctcgt
     7861 cgccctgacg caccaccggc tcgcccttgc ggtagtcggc gaccgcgagc agcggctcga
     7921 gctcgcccca ctgcgccgcg gtcaggcctt tcaggagctg gttctgccgc agctgcaggc
     7981 ggatgctgtt gcgctgcggg tggctcggga acgcggtcat cggagccggg attttagacc
     8041 cttgacctga gtcaaggccc gctgcggggt gcgcttcata ccataccgtt cccctcgagg
     8101 aggccaccat gggcaccgtc gaaccgcaag tcgccgccga tgcgcaagcc gccgtcaccg
     8161 acggctttca gctggtcatc gatgcgctga agctgaacgg catcgatacg atctacggac
     8221 tgcccggcat tccgatcacc gacctcacgc gccgcgcgca ggccgacggc ctgcgcgtgc
     8281 tgtcgttccg ccacgagcag aacgcgggct acgccgcgtc gatcgccggg ttcctcacgc
     8341 aaaagccggg catctgcctg acggtgtccg cgccggggtt cctgaatggc ctcaatgccc
     8401 tggcccacgc gaccaccaac tgcttcccga tgatcctgat cagcggctcg agcgagcgcg
     8461 agatcgtcga cctgcaacag ggcgactacg aggagatgga ccagctcgcc atcgccaagc
     8521 cgctcgccaa ggcggcgttc cgtgtgctgc acgcggagga catcggcgtg ggcgtggcgc
     8581 gcgccatccg cgcggcggtc tccgggcgtc cgggcggggt gtatctcgac ctgccggcga
     8641 agctctttgc ccagggcatc gacgccgcgg ccgggaagaa ctcgctgatc aaggtcgtcg
     8701 acccggcgcc gcggcagatt cccgcgcccg atgccgtcaa gcgggcgctg gacctgctga
     8761 aaggcgcgaa caagccgctc atcattctcg gcaaaggcgc ggcctacgcg caggcggacg
     8821 ccgacatccg cgcgctggtc gagaagaccg gcattcccta tctgccgatg tcgatggcca
     8881 aggggctgct gcccgacacg cacgagcagt gcgcgtccgc cgcgcgctcc ttcgtcctgc
     8941 ccggcgccga cgtggtgatg ctgatcggcg cgcgcctcaa ctggctgctg tcgcatggca
     9001 agggcaagac ctggggcggc aagtcgcaca aggactgggg cgggcagaag ttcatccagg
     9061 tcgacatctc gcccaccgag gccgacagca acgtgcgcat cgatgcgccg gtggtgggcg
     9121 acatcggctc gtgcgtccag gcgctgctcg ccggcgtcgg cgcgggctgg gcgaagccgc
     9181 ccgcggactg gctgaacgcg atccaggaga agaagtccac caacgtcgcg aagatggccg
     9241 agacgctggc gaagaacccg tcgccgatga acttccacag cgcgcttgcc gtggtgcgcg
     9301 acatcgtgaa agcgaatccg gacgcgatgt tcgtgaacga gggcgcgaac gcgctcgact
     9361 tcacgcgcag catcgtcgac atgtacaagc cgcgcaagcg catcgacgtc ggcacctggg
     9421 gcatcatggg cgtcggcatg ggctactgcg tcgccgccgc agtggtcacg aagcagcagg
     9481 tgatcgcgat cgagggcgac agcgccttcg gcttttccgg catggaagtc gagaccatct
     9541 gccgctacaa gctgccggtg tgcgtggtga tcatgaacaa caacggcgtg tacaagggca
     9601 ccgacgtcaa cccgcggggc ggcgacgtcg cgcccacggt gttcgtcaag gatgcgcgct
     9661 acgaaaagct gatggaggcc ttcggcggcg tgggcgtgta cgccaccacg ccggaggagc
     9721 tgcgcaaggg catggaggac gccatccgct cgcgcaggcc gacgctcatc aacgccgtga
     9781 tcgacgagac cgccggcacc gaaagcggcc gcatcacgag cctgaacccg agcgcgaaga
     9841 agaagtaggt ttccggaggg gagaatggaa gcactcaagg gagtccgcat cctcgacatg
     9901 tcccacgtgc aggcgggccc gacctgctcg cagctgcttg cctggctggg cgccgacgtg
     9961 atcaagttcg agaacccggc gggcgacgcg acgcgcgggc agctgcgcga cgtgccgaac
    10021 gccgacagcc tgtacttcac gatgctcaac tgcaacaagc gctcgatcac cgtgaacatg
    10081 aagagcgccg agggcaagca ggtgttcgtc gacctgctga agaagtgcga catcatcatg
    10141 gagaacttcg gccccggcgt gctcgaccgt ttcggcttca cctgggagaa gatccacgag
    10201 ctcaacccga ggatcgtcat gggctcgatc aagggcttcg gctcgagcgg gccctacgcc
    10261 gacttcaagg cctacgagaa cgtggcgcag gcgatgggcg gggccatgag caccaccggc
    10321 acacccgacg gcccgccgtt cgtgacgggc gcgcagatcg gcgactcggg caccgggctg
    10381 cacctcgcca tcgggctgct tgcggcgctg cgccagcgcg atctgaccgg caagggccag
    10441 tatgtcgaag tcgcgatgat ggacggcatc atgaacctgt gccgcgtgaa attccgcgac
    10501 catcagcgcc tgacgcgcgg ctcccttagc gagtattcgg tgggcacgca caagggcatg
    10561 ggcgcagtgc cgcgcgccgg aaacgactcg ggcggcggcc agctcggcaa cgcggtgcgc
    10621 tgcaagccgg gcggcccgaa cgacttcctc tacatcgtgg tgcaggaggc ggtgtggagc
    10681 gcgctcgcca agctgatcgg gcccgaggtc ggcatgccgg atctggcgac cgaccccaag
    10741 ttcgccaaga tcgccgatcg ccgcctgaac cagaacgaca tgtgggaaat catcggcgag
    10801 ttcgcgctca agcatacgaa gcgggaactg atggcgctcc tgaaccctct ggacgtgccc
    10861 tgcgggcccg tcatgtccac cgaagacctc acgcacgacg agcacgtgcg cgggcgcgag
    10921 atgtgggtcg agctcgacca tcccgagcgc gggccctggt acaacgtcgg catgccgatc
    10981 aagctgtcgg actcgcccgc ggtgatcaag cgctcgccaa cgctcggcga gcacaccgac
    11041 gaggtgctga aggaggtgct gggctatgac gagggccgga tcaatgccct gaagagcgcc
    11101 ggcgcgttca ccgcacctcc gaagaaggct gcctgatgaa aatcgccatc gtcggtcagc
    11161 aggatttcgg caaagcggtg ctcgaggcgt tcctcgcgcg cggcgaccaa gtggcggcgg
    11221 tgttctgcgc gccggagaag gagggcgcgc gccccgacgc gctgcgcgcc gcggcgcagg
    11281 aaaagggcct caaggtgcac cagttcaagt cgctgaaggc cccggaggcg gtgcaggcga
    11341 tgaaggacgc gggcgcgcag atcggcatca tggcgttcgt gctgcagttc gcgccgcagg
    11401 agttcgtcac gctgcccaag cacggcacca tccagtacca cccgtcgctg ctgccgaagt
    11461 accgcgggcc gtcgtcgatc aactggccga tcagccgcgg cgacaaggag accggcctca
    11521 ccatcttccg cccgaccgac gggctggacg aaggcgccgt catcctgcag aagaagacgc
    11581 cggtcggcga caacgacacg ctgggcaacg tgtatttcga gcgcctgttc ccgatgggcg
    11641 tggaagcgat gctggaggcg gcggacctgg tcgttgccgg caagcacaag gagactgcgc
    11701 aggacgagtc gcaggcctcc tacgagggct ggttccgcga ggccgaagcc aagatccact
    11761 gggcaaacca tgtcgacctg atccacaacc tgatccgggc gagcgacccg gccccgggcg
    11821 cctggaccac gctcgggggc gtcaagctgc agttgttcga cagccgcaag gaaaccgtgc
    11881 gcacgtttgg cgccgtaagg ggcaagatcg gcgaggtgct gggcctcgaa ggcgggcgcc
    11941 tggtgatcac cgcgcagggc gggcgcatcc acgctggcaa ggtgaagctc ggcgacggca
    12001 agaagctcgc cgctgccgag gccggcatcc aggcggggca gattctcggt acctgatccg
    12061 taaatatttg acgcagctta gttttgactg caagaatgcg gtccagacgg ctgtttcaaa
    12121 gggcgttgtc gggggctggg ccgccttgat cgacaacagg ggatcgggga gggccctggc
    12181 atgaaagcgt cattctggaa gtccgactgg ttcctcggcc tggtggtcgt ggtcgtgatg
    12241 ctgttcgcgg gcggcagcga cctgatgcag agcctcgagc gcaaagccta cgacatcggc
    12301 gtgcagagct ccgcgcgtgc gccgtcggac cgcatcgcgg tgatcgcaat cgacgacacc
    12361 tcgatcgcca atatcggccg ctggccgtgg tcgcgcgagg tgcacgcgcg catgaccgat
    12421 ctgctggcgg gggcccgagc caaggtgatc gccaacacgc tgttcctgtt cgagccgcag
    12481 ctcgaccccg gctaccagta catcaccaag ctgctcgagc tggccgcgcg cgccgacgcg
    12541 gcggcgcccg ccacgcccgc ccaggccgag atgctcgcgc tcctgcgctc ggcagaggcc
    12601 gcgctcaaca ccgaccggcg cctcgcggaa agctacgcgc gcgccggaaa cgtcctgctg
    12661 cccatgctct tcaccatcga catcccgcgc ggcaagcccg accggccgct gcccgacttc
    12721 gtggcgaaga acgcgattcc cgccgggcag cccgagctcg gcgcgcttcc ggcggcggag
    12781 gtgtattttc ccctcgagaa cctcggccgc gcggcggcgg cgctcgggca cctcaacgcc
    12841 aacccggacg tcgacggcgg catccgcacc gagccgctgg tgctgcgcta cttcgagcag
    12901 cacttcccct cgctctcgct gatgatcgcc gcgaagagcc tgaacctctc gcccgacgac
    12961 atcacgatcc gcgccaacga gggcgtcacg ctcgggcggc tcagcatcgg caccgacccc
    13021 gcgctgcaga tgtacaccta cttctacaag aacgtcgacg ggcgacccgc cttccaggtg
    13081 gactcgttct acgacgtgat caccggcaag atcccggcgg cgaagtacgc cgacaagatc
    13141 gtgctgatcg gcgccaccgc ggcgggtgtg ggcaccacct ccgtgacgcc ggtgtccccg
    13201 gcaatggcgc cggtgctgac gctggcgcat tcggtgtcct cgatcctgca ggagcacttc
    13261 ttcgtggcgc cggcctgggc ctacctcgcc tcctggggcg cgttcctgct cgtggcgctg
    13321 tacctgatca tcctgctgcc gcgcctcagc gcggcgccgg cggcgctggt gacggtcctg
    13381 gtgcttggcg tgctgatcgg cgtgcacttc gtcctcatga cgacgcagaa cacctggatg
    13441 cagctgatgg tgccggcgtc gctgctgctc gtgggccacg tcgcgctggt gaccaagcgc
    13501 ttcatcgtca ccgaggccgc gaaggtgaaa tcggacgagt cctccgccga gtcgaaccgc
    13561 atgctcggca tcgcctacca ggcgcagggg cagctggatc tcgcctggga caagttccgc
    13621 caggtgccga tgtcggcggc ggtgatggac aacctgtaca acctggcgct cgacttcgag
    13681 aggaagcgcc agttcaacaa ggcggaatcg gtcttccgcc acatgcacga ccacaacccg
    13741 aaattcaagg acctcgcgag ccgcctgtcg cgcgccaagc agctctcgga aaccgtgatc
    13801 atcggcggcg gctcgaccca cccgggcggt tcgctcaacc tcgccgcggg cgagaagccc
    13861 aagttcggcc gcttcgtggt gcaaaaggaa ctgggcaagg gcgccatggg cgtcgtgtac
    13921 ctgggcaagg atccgaagat cggccgcgag gtcgcgatca agacgatggc gctggcgcag
    13981 gagttcgagg ccgacgagct cgccgaggtg aaggagcggt tcttccgcga ggcggagacc
    14041 gcggggcgcc tctcgcaccc gaacatcgtc accatctacg ataccggcga agagcacgac
    14101 ttctgctaca tcgccatgga gctgctcaaa ggcggtgacc tcgcgccgta tgtcaaggcg
    14161 ccgaacctgc tgcccggcga caaggtggtg tcgatcgtgg cgcgtgcggc cgacgccctc
    14221 ggctacgcgc acaagcaggg cgtcgtgcac cgggacgtca agccggcgaa catgatgtac
    14281 cacccggaca gcgacacgct gaagctcacc gacttcggta tcgcgcgtct caccgattcg
    14341 tcgaagacca agaccgggat ggtgctcggc acgccgtcct acatgtcgcc cgagcagctc
    14401 gccggcaaga gaatcgaggg gcgctccgac ctgttctcgc tggcggtgag cctgtaccag
    14461 ttgctgtgcg gcaggctgcc gttcgaaggc gagtccatgg cgcagctcat gttcaagatc
    14521 gcgagcgagc cgccgaccag catcctgacg gtcaatccgc gcgtgccgcc ggcactggtg
    14581 gcgtttctgg acaaggcgct cgccaagaat cccgacgcgc gctatcaatc gggcgaggag
    14641 ttcgccgccg cgctgcgtgc ggcggccggc gccgcggggg gcgcgcgacc ggcggcgccg
    14701 gccggcggcc cgcccagaag cgtggacatc gagctctgag cccatggatc tgagccaggt
    14761 cctcgagatc gcgtcgtgca ccgacccggg catggtgcgc tcgcacaacg aggattcgat
    14821 cgccgccgat cccgccagcg gcctggtggt gctcgccgac ggcatgggcg gctacaacgc
    14881 cggcgaggtc gcgagcggca tggcgaccac ggtgatcacc acggagatcc ggcagctgct
    14941 cgccaaggtg aggccctacg acgtcgatcc ggaatcgaac aagatcatcg ccggacagat
    15001 ggtgcgcgag caggtgatga aggccaacac ctcgatctac caggccgcgc agagccagcc
    15061 gcagtacgcg ggcatgggca ccacgctcgt ggtgtgcctg ttctacgaca acaaggcgct
    15121 ggtggcgcac cttggcgact cgcgcctgta ccgcatgcgc gacggcacgc tcaagcaggt
    15181 gacgcgcgac cattcgctgc tgcaggagca gatcgactcg ggcctgatca cggcggagca
    15241 ggccaagcac gcgcagcaca agaatctcgt cacccgggcg ctcggcatcg acccggcggt
    15301 cgagcccgag attcgagagt acgagacgcg gcccggcgac atctacctgc tgtgctcgga
    15361 cgggctgtgc gacatggtca cggacgagga catcagcatg acgctggagg cgctcggcgc
    15421 aaacctgaag ctcgccgcgc agcagctggt gcagatggcc aatgacaacg gcggcaaaga
    15481 caacgtttcg gttatactcg tgcgcgtcca gcgggagtac cccgccgccc gcggcgtgat
    15541 ggggaagttt ctaggatggc taaaataggc tccaggggtc ggctatggcg aaactgatac
    15601 tcagcatgga cgggctggtg ctcaaggaga tccccttgag caaggagcgc accaccatcg
    15661 gccgcaagcc gcacaacgac atccagatcg acaacctcgc agtgagcggc gagcacgcgg
    15721 tgatcgttac catcctgaac gactgcttcc tcgaggacct cggcagcacc aacgggaccc
    15781 tggtcaacgg caacccggtc aagaagcact tcctgcagaa caacgacgtc gtcgagctgg
    15841 gcaagtaccg cctgaagttc gtcggcgagg cgccggtgcc ggcggccacc gccgagaggg
    15901 cggacttcga gaagaccatg gtgctgcgcc catcggcgat gaaggcggcc gccgaccagg
    15961 cgcgggcgat gggcgcgggc ggcgccgagg cggcgcgcgc gtcggctgcg cagcaggccg
    16021 cggccagcgc gacctccatg ggcactgccg agagggccac gcccacgccg acgccatcgc
    16081 cgacggccac cacgccgatg tcgtcgccag cggccaccac gccgacgccc atcccggcgc
    16141 tggcgcggcc ccccgccgcc gccccggccg ccgcg
//
LOCUS       DBNHKE010000182         4913 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_349245, whole genome shotgun sequence.
ACCESSION   DBNHKE010000182 DBNHKE010000000
VERSION     DBNHKE010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_349245"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..497)
                     /locus_tag="AC16O9_12610"
     CDS             complement(192..497)
                     /locus_tag="AC16O9_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6517495.1"
                     /translation="MNKRLRKELLESARGLYELGAIDTKTMREFEALEVPTPKTYSAQ
                     EIKKLRLKERASQAVFAAYLNTSVSTVQKWEIGEKKPSGAALKLLDLVDRKGLKALA"
     gene            complement(494..862)
                     /locus_tag="AC16O9_12615"
     CDS             complement(494..862)
                     /locus_tag="AC16O9_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR6517496.1"
                     /translation="MRVLKAKTFARWARREGLPDRVLRSAVEEVRNGLVDADLGGGLA
                     KKRIARPGAGKSGGYRVLLATDLRDRWVFLTGFAKSERGNIGEGELYYWRKIAQAHLA
                     MSEEMTLRAIAAGLLVEVEE"
     gene            complement(951..1619)
                     /locus_tag="AC16O9_12620"
     CDS             complement(951..1619)
                     /locus_tag="AC16O9_12620"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6517497.1"
                     /translation="MKDDEFAEARQRMVAEIAAETIFSSGETGKAALDPRVLEALGRV
                     PRHEFVPVELQPYAYVNSPLPIGCDKTISQPFICALMSDLLEPRPGDRVLEVGTGLGY
                     QAAVLSGLVHAVYSVEFVPELHAQAKRRLAKIGNVELRLGNGYYGWPEHAPFDKIICT
                     AAPELVPPPLLQQLKPGGRMAIPAGLQDAQTLMLVEKNESGKTSIREVLRVRFSLLEG
                     TDNR"
     gene            1646..3736
                     /locus_tag="AC16O9_12625"
     CDS             1646..3736
                     /locus_tag="AC16O9_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6517498.1"
                     /translation="MGYLVGIDIGGTFTDCAIVDRAGRLLTTKVPSTPQDFSQGMMDA
                     LLTGARALDLTLDDFCRDIAFLSHGTTVGTNTIIQKKGAKVGLITTRGHEDAIHIMRG
                     SRGYGGRDIRKVVHFPETSKPAPIVPKRLIRGVSERVDCFGEVVAPLNEAEAENAIRE
                     LVAAGVQAIAVCFLWSFRNPKHELRVRELVRKLAPEVFVTCSVDIAPKWGEYERVTAT
                     ALNAYLGPVMSGYLAKLDRTLTGLGYAHGLQIAQCGGGTVPVARAGEAPLLTLDSGPV
                     AGVTASSFLGAAMGEKHIITTDMGGTSFDVSIIYDGKPAYAFTSNTDQYEYFLPKVDI
                     QAIGAGGGSLAQAHPETRTLTVGPESAGAFPGPVCYGRGGTVPTVTDAQLVLGYLDPD
                     NFAGGRMKLDHQAAHAAIEALGAKIGMGAVQCAAGICRIVELNMADIIRKVTVEKGYD
                     PRDFVLFAFGGAGPAHAGVFAAELGVKKVIVPQRKAASTWCAFGAAAADVLHIFEHSE
                     ILATPVRAGQLNANLEAIEKRARAMMAGEGIAAARQRFEFSLDVRHRGQINEVEVLIP
                     WKRMPADYEPRLRKLFVERYEKLYGRGSALAGAQLEIVVCRLRARALTPRPKLTRARS
                     ATRAIPKGALLKPRSIWWQGLRKTPVYDGEKLAAGNVIRGPAIVETADTTVVVHPGTR
                     LRVDALDNFEITFK"
     gene            3754..>4913
                     /locus_tag="AC16O9_12630"
     CDS             3754..>4913
                     /locus_tag="AC16O9_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6517499.1"
                     /translation="MNAPLRKLSELTGIAFDGVRTGYVPPKKLSLSPKLKLHRKSRRD
                     IDPVTFEVVRHALWNVNEEHGATIQRLSGSPVAMYALDLNPSILTEDAEFVYFGPYMQ
                     YMSGVTDTQVKWTLENRSDNPGIRDGDMFLANDPWVGAAHQMDVMLLCPVFWKGELFC
                     WVTNCLHQYDIGGITPGSFCPSARDAFDEGILIPPVKIVEGNEIRRDIEEIYLRSSRK
                     PSAVALDLRAQFAGNMTGRDRILALIRRYGADTVKGVMKKIIDDGERAFLKKLARLPD
                     GVWRDRTYVECCRPGDRGVHRVMLSLRKEGNKLTFENEGTDAQAGAINATYSGWRGAI
                     MVALNELLCWDQYYAVGGALRHVDFNPTPGTLNCADFPASVSTAPVQCMEISL"
BASE COUNT          799 a         1713 c         1623 g          778 t
ORIGIN      
        1 gaagaagaat ttcatcgagg cgcaggcggc gctggaggca gcgctcgcgc gcctgctcgg
       61 cgacgcggct acgcgcacgc cggacctggg cggcgcgctc ggcacgcgcg cgttcggcgc
      121 ggccctcgcc ggacagatcg agggacgcag ctagcgggga cggctcagga ccagtaggcc
      181 gggcgaggct gctacgccag cgccttcagt cccttccggt ccaccaggtc gagcagtttc
      241 aatgccgctc cactcggctt cttctccccg atctcccact tctggactgt cgacacgctc
      301 gtattgagat aagccgcgaa caccgcctgg ctggctctct ccttcagccg cagcttcttt
      361 atctcctgcg ccgagtacgt cttgggggtc ggcacctcca gcgcttcgaa ctcgcgcatc
      421 gtcttggtgt cgattgcgcc aagctcgtag agcccgcggg ccgattcgag caattccttg
      481 cgaagtctct tgttcactct tccacctcta cgagcaaccc ggcggcgatc gcccgcaggg
      541 tcatctcctc gctcatggca agatgcgctt gcgcaatctt ccgccagtag tacaactctc
      601 cctcgccgat gttgccgcgc tcactcttgg cgaatccggt gagaaagacc catcgatccc
      661 gcagatccgt tgccagcagc acccggtagc cgccgctctt gcccgccccg ggtcttgcga
      721 tccgcttctt cgccaggccg ccgcccagat cggcatcgac cagtccgttt cgcacttctt
      781 cgacagcgga tcgcaaaacg cggtcgggca gtccttcccg gcgcgcccag cgtgcgaacg
      841 tcttcgcctt caggacgcgc atgacactgc gagctggact atatcactag gtgatatggt
      901 atacaaggcc atccgcggtc cccctcttgg ggacttaacg cacgacagcc ctatcggttg
      961 tcagtcccct ccaatagcga gaaccgcacc ctgaggacct cccgaatgga ggtcttccca
     1021 gactcgttct tctccaccag catcagcgtc tgcgcatcct gcagccccgc cgggatcgcc
     1081 atgcgcccgc cgggcttgag ctgctgcagc agcggcggcg gcacgagctc gggcgccgcg
     1141 gtgcagatga tcttgtcgaa cggcgcgtgc tcgggccacc cgtagtagcc gttgcccagc
     1201 cgcagctcga cgttgccgat cttggcgagc cggcgcttgg cctgcgcgtg cagctcgggg
     1261 acgaactcca cgctgtacac ggcgtgcacc agcccggaga gcaccgcggc ctggtagccg
     1321 aggccggtgc cgacctcgag cacccggtcc ccgggccgcg gctcgagcag gtcgctcatc
     1381 agcgcgcaga tgaaaggctg cgagatggtc ttgtcgcagc caatcggcag cgggctgttg
     1441 acgtaggcat aaggctgcag ctccaccggc acgaactcgt gccgcggcac gcggcccagg
     1501 gcctccagca cccgcgggtc gagcgccgcc ttgccggtct cgccgctcga gaagatcgtc
     1561 tcggcggcga tctcggccac catgcgctgg cgggcctcgg cgaactcgtc gtccttcatc
     1621 gcgatccgcg gctaatatag cgccgatggg ctacctcgtc ggcatcgaca tcggcggcac
     1681 gttcaccgac tgcgccatcg tcgatcgggc gggccggctc ctcaccacca aggtcccctc
     1741 gacgccgcag gatttctcgc agggcatgat ggatgcgctg ctgaccggcg cgcgggcgct
     1801 cgatctcacg ctcgacgact tctgccgcga catcgcgttc ctgtcgcacg gcaccacggt
     1861 cggcaccaac accatcatcc agaagaaggg cgccaaggtc gggctgatca ccacccgggg
     1921 ccacgaggac gcgatccaca tcatgcgcgg ctcgcgcggc tacggcgggc gcgacatccg
     1981 caaggtggtg catttcccgg aaacgtccaa gcccgcgccg atcgtcccca agcgcctgat
     2041 ccgcggcgtt tccgagcgcg tcgactgttt cggcgaggtg gtggcgccgc tgaacgaagc
     2101 cgaagccgag aacgcgatcc gcgagctcgt cgccgcgggc gtgcaggcga tcgccgtctg
     2161 cttcctgtgg tcgttccgca acccgaagca cgagctgcgc gtgcgcgagc tggtgcgcaa
     2221 gctcgcgccg gaggtgttcg tcacctgctc ggtggacatc gcgcccaagt ggggcgagta
     2281 cgagcgcgtg accgcgacgg cgctcaatgc ctacctcggc ccggtaatgt cgggctacct
     2341 ggcgaagctc gaccgcacgc tgaccggcct gggctacgcg cacgggctgc agatcgcgca
     2401 gtgcggcggc ggcacggtgc cggtggcgcg cgcgggcgaa gcgccgctgc tcacgctcga
     2461 ctcggggccg gtcgcgggcg tcaccgcctc gagctttctc ggcgcggcga tgggcgagaa
     2521 gcacatcatc accaccgaca tgggcggcac ctcgttcgat gtctccatca tctacgacgg
     2581 caagcccgcg tacgcgttca ccagcaatac cgaccagtac gaatatttcc tgccgaaggt
     2641 cgacatccag gcgatcggcg cgggcggcgg cagcctcgcg caggcgcatc ccgagacgcg
     2701 cacgctcacc gtgggcccgg agagcgcggg cgcgtttccc ggcccggtgt gctacgggcg
     2761 cggcggcacg gtgcccacgg tcaccgacgc gcagctggtg ctcggctacc tcgatccgga
     2821 caatttcgcg ggcgggcgca tgaagctcga ccaccaggcg gcgcacgcgg cgatcgaggc
     2881 gctgggcgcg aagatcggca tgggcgcggt gcaatgcgcg gcgggcatct gccgcatcgt
     2941 cgagctcaac atggccgaca tcatccgcaa ggtcacggtg gagaagggct acgacccgcg
     3001 cgatttcgtg ctgttcgcct tcggcggggc gggtccggcg cacgcgggcg tattcgccgc
     3061 cgagctgggg gtgaagaagg tgatcgtgcc gcagagaaag gccgcgtcca cctggtgcgc
     3121 cttcggcgcg gcagccgcgg acgtgctgca catcttcgag cacagcgaga tcctcgccac
     3181 gccggtgcgc gccgggcagc tgaacgccaa cctggaggcg atcgagaagc gcgcgcgcgc
     3241 catgatggcg ggcgagggca ttgccgccgc gcggcagcgc ttcgagttct cgctggacgt
     3301 gcgccaccgt ggccagatca acgaggtcga ggtgctgatt ccgtggaagc gcatgcccgc
     3361 ggactacgag ccgcggctca ggaaactctt cgtcgagcgc tacgagaagc tctacggccg
     3421 cggctcggcg ctcgccggcg cgcagctcga gatcgtggtg tgccggctgc gcgcgcgtgc
     3481 gctcacgccg cgcccgaagc tcacccgggc gcgcagcgcg acgcgggcga ttccgaaggg
     3541 cgcgctgctc aagccgcgca gcatctggtg gcagggcctg cgcaagacgc cggtgtacga
     3601 cggcgagaag ctggcggccg gcaacgtcat ccgcgggccg gcgatcgtgg agactgcgga
     3661 caccaccgtg gtggtgcatc ccggcacgcg ccttcgcgtc gacgcgctcg acaacttcga
     3721 gatcaccttt aaatagggac agacctcatt taaatgaacg ctcccctcag gaagctcagc
     3781 gagctgaccg gcatcgcgtt cgacggcgtg cgcaccggct acgtgccgcc gaaaaagctc
     3841 tccctgtcgc ccaagctgaa gctgcaccgc aagtcccggc gcgacatcga ccccgtcacc
     3901 ttcgaggtcg tgcgccatgc gctgtggaac gtgaacgagg agcacggcgc caccatccag
     3961 cgcctctcgg gctcgccggt ggcgatgtac gcgctcgatc tcaacccctc gatcctcacc
     4021 gaggacgccg agttcgtcta tttcggcccg tacatgcagt acatgtccgg cgtcaccgac
     4081 acgcaggtga aatggacgct cgagaaccgc agcgacaacc cgggcatccg ggacggcgac
     4141 atgttcctcg ccaacgaccc ctgggtgggc gcggcgcacc agatggacgt gatgctgctg
     4201 tgccccgtgt tctggaaggg cgagctgttc tgctgggtga cgaactgcct gcaccagtac
     4261 gacatcggcg gcatcacgcc gggaagcttc tgcccctcgg cgcgcgacgc cttcgacgag
     4321 ggcatcctca tcccgccggt gaagatcgtc gaggggaacg agatccgccg cgacatcgag
     4381 gaaatctacc tgcggtcctc gcgcaaaccg tcggcggtgg cactggattt gcgcgcgcag
     4441 ttcgccggca acatgaccgg gcgcgaccgc atcctcgcgc tgatccgccg ctacggcgcc
     4501 gacacggtga agggcgtgat gaagaagatc atcgatgacg gcgagcgcgc gttcctgaag
     4561 aagctggcca ggctgcccga cggcgtgtgg cgcgaccgca cctacgtcga gtgctgccgc
     4621 ccgggcgatc gcggcgtgca ccgcgtgatg ctgagcctgc gcaaggaagg caacaagctc
     4681 accttcgaga acgagggcac cgatgcgcag gccggcgcga tcaacgccac ctattcgggc
     4741 tggcgcggcg cgatcatggt ggcgctgaat gaactgctgt gctgggacca gtactacgcc
     4801 gtgggcggcg cgctgcgcca cgtggacttc aaccccacgc cggggacgct caactgcgcc
     4861 gacttcccgg cctcggtctc caccgcgccg gtgcagtgca tggagatctc gct
//
LOCUS       DBNHKE010000183         7571 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_190433, whole genome shotgun sequence.
ACCESSION   DBNHKE010000183 DBNHKE010000000
VERSION     DBNHKE010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_190433"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="AC16O9_12635"
     CDS             <1..472
                     /locus_tag="AC16O9_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR6517500.1"
                     /translation="LAALLGFAAGLGWRPAFSAPGSEAARAMFSAPAAFQGRLFVAYP
                     TRGDLQGGSPAAGAFEQLRSERALGQGHAAAQGFAYAALSITLEGLRRAGRELNRERF
                     ARALEGLHRFDAGPTPPVSFGPGRRVGALGGYVLAVDPQRGFRPVSDWIGLEPL"
     gene            504..1163
                     /locus_tag="AC16O9_12640"
     CDS             504..1163
                     /locus_tag="AC16O9_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6517501.1"
                     /translation="MNFGNFTSRLALALVLAGAAPAHTGAHTPAADAGKAKAQGEPAQ
                     DVRARPDARAYFTDLELLTHEGRKVRFYSDVLAGRTVLINVIYTNCKDACPLITQHLN
                     QVRGLLGDRFGREIMFVTLSSDPERDSPAALRGFAEAQNADLSGWVFLTGAKSNIDHI
                     LKKLGQFSPSVEAHSTLLIAGNVPAKRWAKIRPDAPPIAIAERLKLLADSPRPGAPAA
                     R"
     gene            1182..2123
                     /locus_tag="AC16O9_12645"
     CDS             1182..2123
                     /locus_tag="AC16O9_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6517502.1"
                     /translation="MRSIRFFTGACLALAAAGAIAQVTPGTAARVNGAAISIQQLERG
                     FEQELRARNLNIAAMRRPETVAEIKREVLERLIGEELLWQKAQRDGLVVGDADVDQAV
                     TRTVAQFKSRDAFERRIARDGFDAKSYREYARRMLSADRAAQALLEGKLAVGDEELER
                     FYRDNPAHFHRPEQLRLRVLQVRAAPGARERIEALRARLEKGEEFDALARRESDHPTR
                     PWGGAMDPVARGQLAGPLGAAAFALQPGELSGVVEAPDGLYVLKLEERMPEATVPFAA
                     ARERIREHLVQGRGRQTLEREVAALRAASKVEILLPL"
     gene            complement(2181..2663)
                     /locus_tag="AC16O9_12650"
     CDS             complement(2181..2663)
                     /locus_tag="AC16O9_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517503.1"
                     /translation="MKSMKLKAVTLGVGAALVASLGTAQAASVSECRALVGFKSDYAA
                     WGAVQNNEQYFVANNRVNPCVVKLVLPSAKGKPDNTQTYDINHEMAKDACSMYNYLSS
                     IDSKLDQAKVNEAYVIVSDMHAKLNSLLGTGKLEQAGYDAITPKVTEVQSCIYNLMSQ
                     "
     gene            complement(2985..3878)
                     /locus_tag="AC16O9_12655"
     CDS             complement(2985..3878)
                     /locus_tag="AC16O9_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HIR6517504.1"
                     /translation="MRALAIVGALAIVPLMLCASAGAQDAAPVARILAERCVMCHQGE
                     AAPLGLRLESIDSLLKGGSKGPVVKPGDPAGSELVRRLRGTSQPRMPLTGPPFLSDAE
                     IASVEKWIAAGAPRGGAAADAQPRTRKPGEPLTYADVQPILGARCAKCHTDNGQMGAP
                     PGGLRLNNYANALASGDRARVVPGRPAASELVRRIRGQSLPRMPFDGPPYLSEEEIAL
                     IVRWIEQGARDADGHRAPVPAGARVRLGGTLAGPQQLDELDFEWQGRRREQRAAIGSR
                     VELRGTVGADGRIYAERVRGR"
     gene            complement(3898..4407)
                     /locus_tag="AC16O9_12660"
     CDS             complement(3898..4407)
                     /locus_tag="AC16O9_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR6517505.1"
                     /translation="MPTGKARRAGRRAHSGFGYVWALLAVAIFGIGLGAVGEVWRTQA
                     QREREAELLRVGREYRAALYSYFASSKLGTPAFPTRLEELLEDRRGPVLRRHLRKIYA
                     DPMTASEDWGLLTSTDGRIRGVYSRSGDKPLKTGNFGPQEGAFEQAQTYADWRFEYVP
                     PRVATPQRR"
     gene            complement(4394..4768)
                     /locus_tag="AC16O9_12665"
     CDS             complement(4394..4768)
                     /locus_tag="AC16O9_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR6517506.1"
                     /translation="MPPRAGRGFTLIELIVVMAIVALLLTVALPRYFGHLDRSKEAVL
                     RADLATMRDALDKHYADRGRYPERLEDLATRRYLRAIPVDPITESATTWIAQVDAAHG
                     GVYNVRSGAPGEGENGVRYADW"
     gene            complement(4774..5256)
                     /locus_tag="AC16O9_12670"
     CDS             complement(4774..5256)
                     /locus_tag="AC16O9_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR6517507.1"
                     /translation="MHAAPRAAGFTLIELVITLAILALLASVAVPVSELSVQRSSEQE
                     LRRHLWQIREAIDAYKRAYDEGRVARKLNATGYPPTLETLVEGVEDQRHPDKRRIYFL
                     RRLPRDPLYADASAPAAATWGKRSYASPPDDPREGEDVYDVYSRAPGKGLNGLEYAKW
                     "
     gene            complement(5258..>7571)
                     /locus_tag="AC16O9_12675"
     CDS             complement(5258..>7571)
                     /locus_tag="AC16O9_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR6517508.1"
                     /translation="AHPAGAFMTLLAFPTRSALLLLAAAVLAACATDTTLREGRSLMS
                     AGQYEAGLETFRRASAEAPGNAKLRAEYLTQRDAAVETLLRDADSARRGGRFDEADAA
                     VARAATLGAEEARVRAARTILARDRRHQQVLQEAKDRLQKGEREQAAALTREVLMENP
                     RQREAQNLRRRIDEERHRAGLAAPVLRSRLAKPVTLQFREAPVRQVFDALSRSTGINF
                     IFDREVRPDLRTSIYVTGVAVEDAVDLILLPSQLDKKVLSETSVLIYPGTPAKQREYQ
                     DLAIRTFYLENADVKQALSMVKTMLKTKDVYVDEKLNLLVMRDTPEAIRLAEKLIAAQ
                     DLAEPEVVLEVAVLEISRTRLTELGVKWPENLSLSLVDPTGPPLFLDELRNMGRADLA
                     VSPSPSVTLNARRSRGDSNLLANPRIRVKNREKARILVGDRLPVISAIVTPSTTTAVT
                     TETVTYLDVGLKLEVEPTVHLDDEVAIKIAMEVSTASNRRTTQNGTTVYDIGTRNANT
                     TLRLRDGETQVLMGLIRDDDRRTASGVPGLGDLPILGRLFASHLDDTQKTEVVLSITP
                     RVVRNVQRADVGLSEFWSGTEAVLRSEPIQLRPALQEPTAVKPDAAAGGAPAAAAPAA
                     EAAPSPANDMRLSWAGPASARVGEEVEVELRATISQPVTSASLQFGFDPAVLEVVGIA
                     EGELLKAGAARTTLSHRIDPAGKVLVSIVRAGAPIEGEGPLVKVKLRARAAAEQARMQ
                     VTLAAPVGRGGQRLTANADAALALTVTGAQ"
BASE COUNT         1009 a         2730 c         2706 g         1126 t
ORIGIN      
        1 gctcgcggcg ctgctcggct tcgcggcagg gctgggctgg cggccggcct tttccgcccc
       61 cggctcggag gcggcgcgcg ccatgttctc ggcgcccgcc gcgttccagg ggcgcctgtt
      121 cgtcgcctac ccgacgcgcg gcgacctgca gggcggcagc ccggccgcag gcgcgttcga
      181 gcaattgcgc agcgagcgcg cgctcgggca gggccacgcc gcggcgcaag gcttcgccta
      241 cgcggcgctc tccattaccc tggaagggct gcgccgcgcc gggcgcgagc tcaaccgcga
      301 gcgtttcgcg cgcgcgctcg agggcctgca ccgcttcgac gcgggcccca cgccgccggt
      361 gagcttcggg ccgggccggc gcgtcggcgc gctggggggc tacgtcctgg cggtggatcc
      421 gcaacgcggc ttccggccgg tctcggactg gatcggcctg gagcccctgt gagcaacgaa
      481 tcggaatcga acggaggtca ggcatgaatt tcggcaactt cacgtcgcgg ctggcgctgg
      541 cgctcgtgct ggcgggcgct gcgccggcgc acacgggcgc gcataccccc gccgccgacg
      601 ccggcaaggc gaaggcgcag ggcgagccgg cgcaggacgt gcgcgcccgc cccgacgcgc
      661 gcgcctactt caccgacctc gagctcctca cccacgaggg gcgcaaggtg cgcttctact
      721 ccgacgtgct cgcggggcgc accgtgctga tcaacgtcat ctacaccaac tgcaaggacg
      781 cctgcccgct catcacgcag cacctgaacc aggtgcgcgg cctgctcggc gatcgttttg
      841 gccgcgagat catgttcgtc acgctcagct cggaccccga gcgcgactcg cccgcggcgc
      901 tgcgcggctt cgccgaggcg cagaacgcgg acctctcggg ctgggtcttt ctcaccggcg
      961 cgaagtcgaa catcgaccac atcctgaaga agctcggcca gttctcgccg agcgtcgagg
     1021 cgcactcgac gctgctcatc gccggcaacg tgcccgcgaa gcgctgggcc aagatccgcc
     1081 ccgacgcacc cccgatcgcc atcgccgagc ggctcaagct cctcgcggac agcccgcggc
     1141 cgggtgcgcc cgcggcgcgc tgagggcggg gcgcgttgtc catgcggtcg attcgcttct
     1201 tcaccggggc gtgcctcgct ctcgcggccg ccggcgcaat cgcccaggtc acgcccggca
     1261 cggcggcgcg cgtgaacggc gccgcgattt ccatccagca gctcgagcgc ggcttcgagc
     1321 aggagctgcg tgcgcgcaat ctcaacatcg ccgcgatgcg ccggccggag acggtggccg
     1381 agatcaagcg cgaagtgctc gagcgcctga tcggcgagga gctgctctgg cagaaggcgc
     1441 agcgcgacgg cctggtggtc ggcgacgcgg acgtggatca agccgtcacg cgcaccgtgg
     1501 cgcagttcaa gtcgcgcgac gcgttcgagc ggcgcatcgc gcgcgacggc ttcgacgcga
     1561 agagctaccg cgaatacgcg cgccgcatgc tctcggccga ccgcgccgcg caggcgctgc
     1621 tcgagggcaa gctggcggtg ggcgacgagg agctcgagcg cttctaccgc gacaaccccg
     1681 cgcacttcca ccggcccgag cagctgcgcc tgcgcgtgct gcaggtgcgc gccgcgcccg
     1741 gcgcgcgcga gcgcatcgag gcgctgcgcg cgcggctgga aaagggcgag gagttcgacg
     1801 cgctggcgcg gcgcgagtca gaccacccga cgcgcccctg gggcggcgcg atggacccgg
     1861 tcgcgcgcgg ccagctcgcc gggcccctcg gggcggcggc cttcgcgttg cagcccggcg
     1921 agctctcggg ggtggtcgag gcgcccgacg ggctctacgt cctgaagctc gaggaacgga
     1981 tgcccgaagc gacggtgccc ttcgcggccg cccgcgagcg catccgcgag catctggtgc
     2041 agggccgcgg ccgccagacg ctcgagcgcg aggtggcggc gttgcgcgcc gcgagcaagg
     2101 tcgagatcct gctcccgctc taggcgggcg aaaaaaaagc cccggcatgc cggggcttca
     2161 gttgctgcga agttgccgcg ttattgcgac atcaggttgt agatgcagga ttgcacttcg
     2221 gtgaccttcg gcgtaatggc gtcgtaccca gcctgctcca gcttgcccgt gccaaggagg
     2281 ctattcagct tggcgtgcat gtcgctcacg atgacgtagg cctcgttcac tttggcctgg
     2341 tcgagcttgg agtcgatcga ggagaggtag ttgtacatgc tgcacgcgtc tttcgccatc
     2401 tcatggttga tgtcatacgt ctgggtgttg tccggcttgc ccttcgccga gggcagcacg
     2461 agcttgacca cgcatgggtt gacccggtta ttggcaacga aatattgctc gttgttctgc
     2521 actgcgcccc acgccgcgta gtccgatttg aaacccacca gcgcgcggca ttcgctgacc
     2581 gacgcggcct gcgccgttcc cagcgaggcg accagcgccg ctccgactcc gagggttact
     2641 gccttgagct tcatagactt catggtcctg ctcctttcca tgcgacttcg aaaagcgccc
     2701 ggtccgcttc aagtaggttc gctcacacct ggacctggcg cagaaaaagt cgagcgcccg
     2761 actgcatggc gcagcctaaa cccgcagtgc gccgggcgcc ggttaacaag tgtcctgccg
     2821 atcgaccggc tcagccggcg gtctataaga aaagataact taaacaatgg gttatagact
     2881 tgccggcact ggatgccgcg gccatttgtc gcgtcgccgc acggcaacac gcgcctgcct
     2941 aaacggtacg cccgtcaagc actgcgggcg ccggcgcgcc gaaatcagcg cccgcgaacg
     3001 cgctccgcgt agatgcgccc gtcggcgccg accgtgccgc gcagctcgac acggctgccg
     3061 atcgcggcgc gctgctcgcg ccggcggccc tgccactcga agtcgagctc atcgagctgc
     3121 tgcgggcccg cgagggtgcc gccgaggcgc acgcgcgctc ccgccggaac gggggcccga
     3181 tgcccatccg cgtcgcgcgc gccttgctcg atccagcgca cgatgagcgc gatctcctcc
     3241 tcgctcaggt agggcggccc gtcgaacggc atgcgcggca gcgactgccc gcggatgcgg
     3301 cgcacgagct cgctcgccgc gggccggccc ggcaccacgc gcgcgcgatc gccgctcgcc
     3361 agggcgttgg cgtagttgtt cagccgcaat ccccccggcg gcgcccccat ctgcccgttg
     3421 tcggtgtggc acttggcgca gcgcgcgccg aggatcggct gcacgtcggc gtaggtgagc
     3481 ggctcgcccg gcttgcgggt ccgcggctgc gcgtcggccg cggcgccgcc gcggggcgcg
     3541 cccgcggcga tccacttctc gaccgacgcg atttccgcgt cgctcaggaa cggcggcccg
     3601 gtgagcggca tgcgcggctg gctcgtcccg cgcagccggc gcacgagctc gctgcccgcg
     3661 ggatcaccgg gcttgaccac cggacccttg ctgcctccct tgagcaggga atcgatggac
     3721 tcgagccgca gcccgagcgg cgcggcctcg ccctggtggc acatcacgca gcgctccgcg
     3781 aggatgcgcg ccacgggcgc ggcgtcctgg gcgcccgcgc ttgcgcaaag catcaggggc
     3841 acgatcgcga gggcgccgac gatcgcaagg gcgcgcatcc cgcaacgata gcgcaggcta
     3901 gcgccgttgc ggggtcgcaa cccgcggcgg aacgtactcg aagcgccagt cggcgtaggt
     3961 ctgggcctgc tcgaaggcgc cttcctgcgg accgaagttt cccgtcttga gcggcttgtc
     4021 gcccgagcgg ctgtacacgc cgcggatgcg cccgtcggtg ctcgtcagca ggccccagtc
     4081 ctcactggcc gtcatcgggt cggcgtagat cttgcgcagg tgccggcgca gcacgggccc
     4141 gcgccggtcc tcgaggagct cctccaggcg cgtcgggaaa gcgggcgtgc cgagtttgga
     4201 gctcgcgaag taggaataca gggccgcgcg gtactcccgt cccacgcgca gcagctcggc
     4261 ctcgcgttcg cgctgtgcct gcgtgcgcca gacctcgccg acggcgccca ggccgatgcc
     4321 gaagatggcg acggccagca gcgcccagac gtagccgaac cccgagtgcg cgcgccgccc
     4381 ggcgcgacgg gccttaccag tcggcataac gcacgccgtt ttccccttcg cccggcgcgc
     4441 cgctgcgcac gttgtagacg cccccgtgcg ccgcgtcgac ctgcgcgatc caggtcgtgg
     4501 cgctctccgt gatcggatcc accgggatcg cgcgcaggta gcggcgcgtg gcgaggtcct
     4561 cgagccgctc cgggtagcgc ccgcggtcgg catagtgctt gtcgagcgcg tcgcgcatcg
     4621 tggcgaggtc ggcgcgcagc acggcttcct tggagcggtc caggtgcccg aagtagcgcg
     4681 gcagcgcgac cgtgaggagc agggccacga tcgccatcac cacgatcagc tcgatcagcg
     4741 tgaatccgcg cccggcgcgc ggcggcatcg cactcaccac ttggcgtact ccaggccgtt
     4801 cagtcccttg ccgggcgcgc gcgagtagac gtcgtagacg tcctcgccct cgcgcgggtc
     4861 gtccggcggg ctcgcgtagc tgcgcttgcc ccaggtggcg gcggccggcg cgctcgcgtc
     4921 cgcgtacagg ggatcgcgcg gcagccggcg caggaagtag atgcgccgct tgtcgggatg
     4981 gcgctggtcc tcgacgccct ccaccagcgt ctccagcgtc ggcggatagc ccgtcgcgtt
     5041 cagcttgcgc gcgacgcggc cctcgtcgta ggcgcgcttg taggcgtcga tcgcctcgcg
     5101 gatctgccac aggtggcggc gcagctcctg ctcgctggag cgctgcaccg agagctccga
     5161 gaccggcacc gccaccgagg cgagcagcgc caggatggcg agggtgatca ccagctcgat
     5221 cagcgtgaag cccgcggcgc gcggcgcggc gtgcacgtca ctgcgctccg gtgaccgtca
     5281 gcgcgagcgc cgcgtcggcg ttcgcggtga gtcgctgccc gccgcggccc accggcgcgg
     5341 cgagcgtgac ctgcatgcgc gcctgctccg cggcggcgcg cgcccgcagc ttcaccttga
     5401 cgagcggtcc ctcgccctcg atcggcgcgc ccgcgcgcac gatgctcacc agcaccttgc
     5461 cggccgggtc gatgcgatgg ctgagcgtgg tgcgcgccgc accggccttg agcagctcgc
     5521 cctcggcaat gcccaccacc tccagcaccg ccggatcgaa gccgaactgc agcgacgcgc
     5581 tggtcacggg ctgcgaaatc gtggcgcgca gctcgacctc gacctcctcg ccgacgcggg
     5641 cactggccgg tcccgcccag gacaggcgca tgtcgttcgc cggcgatggc gcggcttcgg
     5701 ctgccggcgc agctgccgcc ggggcgccgc ccgcggccgc gtccggcttg accgccgtcg
     5761 gctcctgcag cgccggccgc agctggatcg gctccgagcg cagcaccgcc tcggtgcccg
     5821 accagaactc ggacaagccc acgtcggcgc gctgcacgtt gcgcacgacg cgcggcgtga
     5881 tcgagagcac cacctcggtc ttttgcgtgt cgtccaggtg gctcgcgaac agccgcccga
     5941 ggatcggcag atcgccgagc cccggcacgc ccgaggcagt gcgccggtcg tcgtcgcgga
     6001 tcaggcccat cagcacctgg gtctcgccgt cgcgcaggcg cagcgtggtg ttcgcattgc
     6061 gcgtgccgat gtcgtagacc gtggtgccgt tctgcgtggt gcggcggttg cttgcggtgc
     6121 tcacctccat cgcgatcttg atcgccacct cgtcgtccag gtgcaccgtg ggctcgacct
     6181 cgagcttgag gcccacgtcg aggtaggtca cggtctccgt ggtgaccgcc gtcgtggtcg
     6241 agggcgtcac gatcgccgag atcaccggca ggcggtcgcc gaccaggatg cgcgccttct
     6301 cgcggttctt cacgcggatg cgcgggttgg cgagcaggtt gctgtcgccg cgcgagcgcc
     6361 gcgcgttgag cgtcacgctc gggctcgggc tgacggcgag gtcggcgcgg cccatgttgc
     6421 gcagctcgtc gagaaacagc ggcgggccgg tcggatccac cagcgagagc gacaggttct
     6481 cgggccactt cacgccgagc tcggtgaggc gcgtgcgcga gatctcgagc accgccacct
     6541 cgagcaccac ctcgggctcg gccaggtcct gcgcggcgat cagcttctcg gcgaggcgaa
     6601 tcgcctccgg cgtgtcgcgc atcacgagca ggttcagctt ctcgtcgacg tacacgtcct
     6661 tggtcttcag catggtcttc accatgctga gcgcctgctt cacgtccgcg ttctccaggt
     6721 agaaggtgcg gatcgcgagg tcctggtact cgcgctgctt ggcgggcgtg cccgggtaga
     6781 tgagcacgct cgtctcggag agcaccttct tgtcgagctg gctcggcagc aggatcaggt
     6841 ccaccgcgtc ctcgaccgcg acgccggtga cgtagatcga ggtgcgcagg tcgggccgca
     6901 cctcgcggtc gaagatgaag ttgatgccgg tcgagcgcga cagggcgtcg aacacctggc
     6961 gcaccggcgc ctcgcggaac tgcagcgtga cgggcttggc caggcgcgag cgcagcacgg
     7021 gggcggcgag cccggcgcgg tggcgctcct cgtcgatcct gcggcgcagg ttctgcgcct
     7081 cgcgctggcg cgggttttcc atcagcacct cgcgcgtgag cgccgcggcc tgctcgcgct
     7141 cgcccttttg cagcctgtcc ttcgcttcct gcagcacctg ctggtggcgc cggtcgcgcg
     7201 cgaggatggt gcgcgcggcg cgcacgcggg cctcctcggc gccgagcgtg gcggcgcgcg
     7261 ccacggcggc atcggcttcg tcgaagcgcc cgccgcgccg cgccgaatcg gcgtcgcgca
     7321 gcagcgtctc cacggcggcg tcgcgctggg tgaggtactc ggcgcgcagc ttggcgttgc
     7381 ccggcgcctc ggccgaggcc cggcggaacg tctccaggcc cgcttcgtac tgcccggccg
     7441 acatgagcga ccggccctcg cgcagcgtgg tgtcggtggc gcacgcggcg aggacggccg
     7501 cggcgagcag caagagggcg gatcgggtgg ggaaagcaag gagggtcatg aaggcacctg
     7561 ccggatgggc a
//
LOCUS       DBNHKE010000184         2083 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_190727, whole genome shotgun sequence.
ACCESSION   DBNHKE010000184 DBNHKE010000000
VERSION     DBNHKE010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_190727"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="AC16O9_12680"
     CDS             complement(<1..142)
                     /locus_tag="AC16O9_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6517509.1"
                     /translation="MYPHLFKPLDLGFVTLPNRILMGSMHTGLEARPDGMERLAAFYA
                     ERA"
     gene            complement(214..1068)
                     /locus_tag="AC16O9_12685"
     CDS             complement(214..1068)
                     /locus_tag="AC16O9_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6517510.1"
                     /translation="MKAADYGRLVALAAIWGAAFIFMRVAAPVLGAIWTPELRVLLGG
                     LALLAWLRATGVDPGLRAHWRSYLIIGGIGIAAPFALYSFAAIHAPASLLAIVNATSP
                     IFGLAWGVLFRDERVTTRKAAGLALGIAGVVLIARPAGLAESPLFGWAVASALGACCL
                     YGVVGVLVKRFSGGATPRGMAAGNQLAAAVVLIPLLPFVPPLAVPSVLVLANVLALAL
                     LASGVAFVLYFRLIADVGATRALTVTYLIPLFGILWGWLFLGEALPAAAFAGGALILS
                     GTVLVTRG"
     gene            complement(1065..1877)
                     /locus_tag="AC16O9_12690"
     CDS             complement(1065..1877)
                     /locus_tag="AC16O9_12690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIR6517511.1"
                     /translation="MEDVLGRARSYLDRHRGERVTLSDLARAVGASPFHLQRRFKQAF
                     GLSPKDYQREAQLEDAKRHLKNGARVTDALFEAGYGSVSRFYEKAAARLGMPARDYRN
                     GGKGARIAYTTFRSPLGTVLIAGTGRGLCAVKLGEGAERLLAEEFSEAELVHDAKALP
                     EVKRRILEFLSGDATLAKIPLDIRGTVFQRKVWNELRRIPAGETRSYGQIARAIGAPR
                     AVRAVGSACGANPVALVIPCHRALRADGSLGGYAWGLAKKKKLLEIEKRTRK"
BASE COUNT          360 a          714 c          723 g          286 t
ORIGIN      
        1 tcgcgcgctc agcgtagaac gccgcgagcc gctccatgcc gtcgggccgc gcctcgagcc
       61 cggtatgcat cgagcccatg aggatgcggt tcggcagcgt gacgaacccg aggtcgagcg
      121 gcttgaaaag gtgcggatac ataaatgggg acagacctca ttttcctatg tgcgaatcga
      181 ttgcaaataa gaaaaatgag gtctgtcccc attttagccg cgcgtcacta gcaccgtccc
      241 actcaatatc agagcgccgc ccgcgaaagc ggccgcaggg agcgcttcgc cgaggaacag
      301 ccagccccag aggatgccga agagcggaat caggtaggta acggtgagcg cgcgcgtcgc
      361 gccgacgtcg gcgatgaggc ggaagtagag cacgaacgcc acgccgcttg cgaggagcgc
      421 gagcgcgagg acgtttgcca gcaccagcac cgagggcacg gcgagcggtg gcacgaaagg
      481 caggagcggg atcagtacca ccgccgcggc gagctgattg cctgcggcca tgccacgcgg
      541 cgtggcgccg cccgaaaagc gcttcaccag cacgccgact acgccataca ggcagcaggc
      601 gccgagcgcc gacgccacgg cccagccgaa cagcgggctt tcggcgaggc ccgccggacg
      661 cgcgatcagg acgacgccgg cgatacccag ggcgaggccc gccgccttgc gcgttgtcac
      721 gcgctcatcg cgaaacagga cgccccaagc caggccgaag atcggcgaag tggcgttcac
      781 gatcgccagc agcgaggccg gcgcgtggat ggcggcgaac gagtagagcg cgaacggcgc
      841 ggcgatgccg atgccgccga tgatcaagta agagcgccag tgagcgcgaa ggcccgggtc
      901 gacgcctgtt gcccgcagcc aagcgagcaa cgcgagcccg ccaaggagca cacgcagctc
      961 cggcgtccag atcgcgccca gcacgggcgc ggcgacgcgc atgaagatga acgctgcgcc
     1021 ccagatcgcg gcgagcgcca ccaggcggcc gtagtcggcc gccttcattt acgtgtgcgc
     1081 ttctcgatct ccaggagctt tttcttcttc gcgaggcccc aggcgtagcc gcccaggctg
     1141 ccgtccgcgc gcagcgcgcg gtggcacgga atgaccagcg cgactgggtt cgcgccgcac
     1201 gcgctgccca cggcgcgcac cgcgcgcggt gcgccgatgg cgcgcgcgat ctggccgtag
     1261 ctgcgcgtct cgcccgcggg gatgcggcgc agctcattcc acaccttgcg ctggaaaacc
     1321 gtgccgcgga tgtctagagg aattttcgcg agggtcgcgt cgcccgaaag gaactcaagg
     1381 atgcgccgct tcacctcggg cagggccttc gcgtcgtgca ccagctcggc ctcgctgaat
     1441 tcctcggcga gcaaccgctc ggcgccctcg ccaagcttca ccgcgcacag gccgcgcccg
     1501 gtgcccgcga tcagcaccgt gcccaggggc gaacggaagg tcgtataggc gatgcgtgcc
     1561 cccttgccgc cgttgcggta gtcgcgcgcg ggcatgccga ggcgcgcggc ggccttctcg
     1621 tagaagcgcg acaccgagcc gtagccggct tcgaacagcg cgtccgtgac gcgggcccca
     1681 ttcttcaagt ggcgcttggc gtcctcgagc tgcgcttcgc gctggtagtc cttgggcgac
     1741 agaccgaagg cctgcttgaa gcggcgttgc aggtggaacg ggcttgcgcc gacggcgcgc
     1801 gcgagatcgc tgagcgtgac gcgctcgcca cggtgccggt cgaggtagct gcgcgccctt
     1861 ccgagaacat cttccatggc ggcactgtag cgggtggcgc gcggcgaatc tatccggatt
     1921 ttgcgctctc gacgtacttg cgcgccgtgc gcacgtcgac gcccatgcgc cgcgcgacct
     1981 ccgcgtagct gccgaggcgc ccatggagca gggcggcgta ctgactcagc agctcgtcgg
     2041 ccgagagctc gcccgcgtgc aagcgcgccc cgagtgcctc ttc
//
LOCUS       DBNHKE010000185        11556 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1354558, whole genome shotgun sequence.
ACCESSION   DBNHKE010000185 DBNHKE010000000
VERSION     DBNHKE010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1354558"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /gene="dxs"
                     /locus_tag="AC16O9_12695"
     CDS             complement(<1..1748)
                     /gene="dxs"
                     /locus_tag="AC16O9_12695"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR6517512.1"
                     /translation="MFELLKTIDDPAQLRALERKDLHQLADELRAFVLDSVSKTGGHL
                     SSNLGTVELTIALHKVFETPEDRIVWDVGHQTYPHKILTGRREAMARLRMIDGISGFP
                     RRIESPYDTFGTAHSSTSISAALGMAAAAKHKGEHRKCVAVIGDGAMSAGMAFEAMNN
                     AGAMDVDLLVILNDNEMSISPPVGALTNYLARLLSGRMYSTARRASEHILKRVGVWEL
                     ARRTEEHVKGMVTPSTLFEEFGFNYIGPIDGHDLDTLVATLGNIAKLKGPQFLHVVTR
                     KGQGYKLAEDDPIAYHGPGKFDPAEGLKKATGGRPSYSQIFGDWLCETAAREPRLVAV
                     TPAMREGSGMVKYSELYPTRYFDVGIAEQHAVTFAAGLACEGLRPVVAIYSTFLQRGY
                     DQLVHDVAIQNLPVTFALDRGGLVGADGATHNGAFDYAFLRTLPNLLIMAPGDENECR
                     QMLQTAITHEGPAAVRYPRGAGPGAALDPVPRALPIGKAEVRRQGRRIAILAFGSMLA
                     PALAAGEALDATVVNMRFVKPLDAALVAELAATHEMLVSVEEHAVMGGAGSAVCEALA
                     AAGQGNRILLLGLPDR"
     gene            complement(1799..2668)
                     /locus_tag="AC16O9_12700"
     CDS             complement(1799..2668)
                     /locus_tag="AC16O9_12700"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6517513.1"
                     /translation="MGGIQARMEAVLSRHLPAAHLAPARLHVAMRYAVLEGGKRVRPL
                     LTFAAGEVAGASPERLEVAAAAVELIHAYSLVHDDLPCMDDDVLRRGKPTVHVEFDEA
                     TALLVGDALQSLAFQLLAEPALADDPRAQLEMVKLLAAAAGSRGMAGGQQIDLAATGK
                     ALSVPELEAMHIHKTGALIRAAVLLGAACGRAPEATLRERLDQYAKTVGLAFQVVDDV
                     LDAEASSATLGKTAGKDSRQGKPTYVSVMGLPRARELAQELRQNAHAALAPLGAGARR
                     LRELADFIVLRKF"
     gene            complement(2688..2930)
                     /locus_tag="AC16O9_12705"
     CDS             complement(2688..2930)
                     /locus_tag="AC16O9_12705"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR6517514.1"
                     /translation="MTKPAAEATFEKALAELEKLVAQMEGGKLSLEQALAAHKRGLEL
                     ARFCQERLDAAQRQVKVLEGEVLKKLAVEPEPGDGT"
     gene            3017..4111
                     /locus_tag="AC16O9_12710"
     CDS             3017..4111
                     /locus_tag="AC16O9_12710"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6517515.1"
                     /translation="MSDLVHARRLQPASCQLPVSWYFDPAVFAEEKARLFDPGPNYIG
                     HELMVPQVGDYHTLAWMDHAKVLVRNAGGVELLSNVCRHRQSLMLEGRGRLQNIVCPL
                     HRWTYDLKGELLGAPHFPESPCVKLASTPLTRWNGLLFAGPADPARTLAGMDTSDWDF
                     SGYVLERVMIDEYDVNWKTFIEVYLEVYHVDPFHPGLGNFTDCKQWHVNYGEDWSVQV
                     AQAKAGLGKPGTPVYRKWHEACLKHLDGRTPKQGALWMAWFPGFMLEWYPNVLIVSQL
                     IPRSPTHTTNVVEFYYPEEIALFEREFVEAQQAAYVETALEDNEICLRMDRGRRALWT
                     QGRDDAGPYQSPMEDTMVHFHEWLRRKLGR"
     gene            4084..4932
                     /locus_tag="AC16O9_12715"
     CDS             4084..4932
                     /locus_tag="AC16O9_12715"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6517516.1"
                     /translation="MAAEKAGALGPLVSTEDLSRHPEWRVFDCRHDLMKPEAGEQLYR
                     EGHIPGASFAHLDRDLSSPKTGKNGRHPLPDPAKFIAWLGSQGLKKTDTVVCYDGGPG
                     AMAARLWWMLRWVGHESVSVLDGGVAKWKKEGRAVTAEVAKFSPTTYPGKARSEMAVD
                     VAHVEKSLGEELVLDARAPARWRGETEPIDPEAGRIPGSLNRFNSDNLTPEGVHKPAE
                     QLRREFEAVLGGRAPAEVVNSCGSGVAACHNILAMEIAGLKDAKLYPGSWSEWIADPK
                     RPRERG"
     gene            4919..5281
                     /locus_tag="AC16O9_12720"
     CDS             4919..5281
                     /locus_tag="AC16O9_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR6517517.1"
                     /translation="MSAADSLLVLHFAIVVFIVGGLLLTWVGALASWAWVRNPWFRYL
                     HLGAIAFVALEAVIGMTCPLTVWEDALRGGGSGESFVGRWVRTLLYYRAPEWVFTVIY
                     VAWTAATLVTLRFVPPRR"
     gene            complement(5250..6539)
                     /gene="hflX"
                     /locus_tag="AC16O9_12725"
     CDS             complement(5250..6539)
                     /gene="hflX"
                     /locus_tag="AC16O9_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR6517518.1"
                     /translation="MTQELAHKPPRAIVAAVQLPSVGDAEFEASLTELRQLAKTLGFE
                     VVSTITQKRASFDATAYFGLGKREELRDLVQQGADLVLVDHEISPSQAFNLGKEVGCE
                     VMDRTMVILEIFHRHARSSAARAQVEIARLGYMGPRMREAAKQAGPKDRARSGTGGRS
                     GGESHGELDRRKIRDRIAELQKELDAMHLERRTQRARRQERQGLARVALVGYTNAGKS
                     TLMRALTASEALVADKLFATLDTTVRALHPESVPRVLVSDTVGFIKNLPHGLVASFKS
                     TLDEALEASLVAHVVDASDPGFERQLAVTEEVLDEIGAKDVPRLIVFNKIDRGGDEAA
                     LREKYPGCVVMSAKREGDVARLREAIVAFFQRRLVEAELYLPWSAQKLRGQIFAGCEV
                     LEERADGAGAFLRVRGEPEALARLREGISAEVARTAA"
     gene            complement(6592..7485)
                     /locus_tag="AC16O9_12730"
     CDS             complement(6592..7485)
                     /locus_tag="AC16O9_12730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6517519.1"
                     /translation="MSDWQPDRLLPGFEAKTFGFPDDYDGRVVATLVRLPAGDAPRGA
                     VLYVHGFVDYFFQRHMAERFAGEGYAFYALDLRKHGRSWLEHQHPCYCKDMAEYDVDI
                     TAAIDEIGGPVLLAGHSTGGLVCALYAHRGARREQITSLWLNSPFFDWKVTPLKRAQL
                     GMAALTGQAFPFLNDPKALKPVYIRSIHRDFDGEWDFDLKLKPLHGFPAYFGWIGAVL
                     AGQHKVHAGLEVECPVLTMHSDEADIVLDWRHVAKWGRTLGPQASVLAFPGGLHDLVL
                     SRREVRDAVFSALFSWAARVH"
     gene            complement(7478..8584)
                     /gene="queG"
                     /locus_tag="AC16O9_12735"
     CDS             complement(7478..8584)
                     /gene="queG"
                     /locus_tag="AC16O9_12735"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR6517520.1"
                     /translation="MPAASAAPRPSVAASSASSRLDSGPMDYGALAARIKQWGGELGF
                     QAVGIADTDLAAAENRLGEWLGRGWHGEMDYMARHGALRARPAELVPGTVRVISCRMD
                     YAATLDEEDDDPLRACVSRYARGRDYHKVLRGRLQKLCERIEAEAGPFGHRVFADSAP
                     VMEVALAAKSGIGWRGKHTLLLSREAGSWFFLGEVYTSLPLPADAPAGEHCGTCTKCI
                     DVCPTQAITGPYQLDARRCISYLTIELKGCIPEEFRPLIGNRVYGCDDCQAVCPWNSF
                     AQPTREPDFAVRNGLDRASLVELFAWSEQAFDERMAGSPIRRIGYERWLRNLAVGLGN
                     APTTPEVLGALRARSDHPSALVREHVAWALSRHE"
     gene            8508..8990
                     /gene="tsaE"
                     /locus_tag="AC16O9_12740"
     CDS             8508..8990
                     /gene="tsaE"
                     /locus_tag="AC16O9_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6517521.1"
                     /translation="MGPESSLELADEAATLGLGAALAAGIAPGRVLHLSGELGSGKTT
                     LVRGLLRELGHSGRVKSPTYPLVELYVVSRLNLYHFDFYRFKDRSEWQDSGFRDYFNA
                     QAACIVEWPERAGGLLPPPDLAVRLEIVPGARRARLSAGTPAGAAWLARLRPRSSSAT
                     "
     gene            8948..10144
                     /locus_tag="AC16O9_12745"
     CDS             8948..10144
                     /locus_tag="AC16O9_12745"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6517522.1"
                     /translation="MAGALAAALLLGYLAAAQAQVIATRIWPARDYTRVTFESRQALD
                     YSMFALKDPQRLVLDLNAEDADGLIAALQSKVAAEDPYVAVLRVGANRPGVTRVVLDL
                     KAEVRPQVFALKPIAEYGHRLVLDLYPLVPPDPLAALLELNGQLAVPGARGGDTTPRS
                     VVRLATVVVDAGHGGEDPGARGRRGAKEKDITLLIARRLKALIDAEPGMRAVLTRDGD
                     YYLSLQARVEKARAVKADLLVSVHADAFTRPHARGSSVFTLSQRPTSAAAAWLAKQEN
                     DSDLIGGVGLDARDPYLKQVLVDLSQTATNDHSMKLAGAVLSELGQINTLHKAQVERA
                     GFAVLKAPDVPSILVETAFISNPQEERRLQDERYQDKMARAMLAGIKRYFSLHPPRPQ
                     GPLAQK"
     gene            complement(10146..10793)
                     /locus_tag="AC16O9_12750"
     CDS             complement(10146..10793)
                     /locus_tag="AC16O9_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR6517523.1"
                     /translation="MELAAALWDFALHLDAHLAAFIAEHGAWVYALLFVIVFCETGLV
                     VTPFLPGDSLLFVVGTLAAAGGMDIVLVMALLVAAALSGDNVNYWIGRWAGPKVFRAE
                     DSRWFNRRHLERTHAFYERHGGKAIIIARFVPIVRTYVPFVAGIGAMPYARFLAFSVA
                     GALLWVVSLSLAGYWFGNIPAVKENLAAVIVVIVVISVLPIAVEFLRARARRATP"
     gene            10818..>11556
                     /gene="mutL"
                     /locus_tag="AC16O9_12755"
     CDS             10818..>11556
                     /gene="mutL"
                     /locus_tag="AC16O9_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR6517524.1"
                     /translation="MSAIRVMPELLVSQIAAGEVVERPASVLKELLENGLDAGATELQ
                     VTLDAGGAKRVQVDDDGAGIAQDDLALALARHATSKIGSLDDLESVGTLGFRGEALAS
                     IAAVARVEIVSRARGAAHAWSVAAEGGAPAAPRPAARAPGTTVCVGDLYFNTPARRKF
                     LRTEATEFGHCDDVFRRIALARPDLAFTLRHNGRVSLHLRPQDLPARAAALLGAEFAA
                     AAVPVDAQAGALRLHGLAGTPQSARSRA"
BASE COUNT         1750 a         4097 c         3959 g         1750 t
ORIGIN      
        1 aaccggtccg gcaggccgag gagcaggatc cggttgccct gtcccgcggc ggcgagcgcc
       61 tcgcatactg cgctgcccgc gccgcccatc acggcatgct cctcgacgct gacgagcatc
      121 tcgtgcgttg cggccagttc ggccaccagc gccgcgtcca gcggcttgac gaagcgcatg
      181 ttcaccacgg tggcgtcgag cgcctcgccg gccgcaagcg cgggcgccag catcgagccg
      241 aaggcgagga tggcgatgcg ccggccctgg cgacgcacct cggccttgcc gatcggcagg
      301 gcgcgcggga ccggatcgag cgccgcgccc gggccggcac cgcgcgggta gcgcaccgcc
      361 gcgggcccct cgtgcgtgat cgcggtttgc agcatctggc ggcactcgtt ctcgtcgccc
      421 ggcgccatga tcagcaggtt cggcagcgtg cgcaggaacg catagtcgaa cgcgccgttg
      481 tgggtcgcgc cgtccgcgcc gaccagaccg ccgcgatcca gcgcgaaggt gaccggcagg
      541 ttctggatcg ccacgtcgtg caccagctgg tcgtagccgc gctgcaggaa ggtggaatag
      601 atcgccacca ccggacgcag gccctcgcag gcgagaccgg cggcgaaggt caccgcatgc
      661 tgctcggcga tgccgacgtc gaagtagcgc gtcgggtaca gctcggaata cttcaccatg
      721 cccgagccct cgcgcatcgc cggcgtcacc gccaccagcc gcggctcgcg cgcagcggtc
      781 tcacagagcc agtccccgaa gatctggctg tagctcgggc gcccgccggt ggccttcttc
      841 agcccctcgg cgggatcgaa cttgcccggg ccgtggtagg cgatcgggtc gtcctccgcg
      901 agcttgtagc cctgcccctt gcgcgtcacc acgtgcagga actgcgggcc cttcagcttg
      961 gcgatgttgc cgagcgtcgc caccagggtg tcgaggtcat ggccgtcgat cgggccgatg
     1021 tagttgaagc cgaactcctc gaagagcgtc gagggcgtca ccatgccttt cacgtgctcc
     1081 tcggtgcgtc gcgcgagctc ccacacgccg acgcgcttga gaatatgttc gctcgcgcgc
     1141 cgcgccgtcg agtacatgcg gcccgagagc agccgagcca ggtagttggt gagcgcgccg
     1201 acgggcggcg agatcgacat ctcgttgtcg ttcaggatca cgagcaggtc cacgtccatg
     1261 gcgcccgcgt tgttcatcgc ctcgaacgcc atgcccgccg acatggcgcc gtcgccgatc
     1321 accgccacgc acttgcggtg ctcacccttg tgcttggccg ccgccgccat gccgagcgcc
     1381 gccgagatcg aagtcgacga gtgcgccgtg ccgaaggtgt cgtagggcga ctcgatgcgc
     1441 cgcgggaacc ccgagatgcc gtcgatcatg cgcaggcgcg ccatcgcctc gcgccgaccg
     1501 gtgaggatct tgtgagggta ggtctggtgc ccgacgtccc agacgatgcg gtcctcgggt
     1561 gtctcgaaca ccttgtgcag cgcgatggtc agctccaccg tgcccaggtt cgacgacagg
     1621 tgcccgcccg tcttcgacac cgaatcgagc acgaacgcgc gcagctcgtc ggcaagctgg
     1681 tgcaggtcct ttctctccag cgcgcgcagc tgcgccgggt catcgatggt cttgagcagc
     1741 tcgaacatca agggattgta gccgccggcc ggcagcgctt cgctcattga cgacacggct
     1801 agaacttcct cagcacgatg aaatctgcga gctcgcgcag ccgccgcgcg cccgcgccga
     1861 gcggcgcgag cgccgcatgg gcattttgcc tgagttcctg ggccagttcc cgcgcgcgcg
     1921 gcaggcccat taccgacaca taggtgggtt tgccctggcg cgaatccttg cccgcggtct
     1981 tgccgagcgt ggcgctggag gcttccgcgt ccagcacatc gtccaccacc tggaacgcga
     2041 ggcccacggt cttggcatac tggtcgaggc gctcgcgcag cgtggcctcg ggcgcgcggc
     2101 cgcaggccgc gcccaggagc accgcggcgc ggatcagcgc gccggtcttg tggatgtgca
     2161 tcgcctcgag ctcggggacc gagagcgcct tgccggtggc ggcgaggtcg atctgctgcc
     2221 cgcccgccat gccgcgcgag cccgcggccg ccgcgagcag cttcaccatt tcgagctggg
     2281 cgcgcgggtc gtcggcgagc gccggctcgg cgagcagctg gaaggcgagc gactgcagcg
     2341 cgtcccccac gagcagcgcg gtggcctcgt cgaattccac gtgcaccgtg ggcttgccgc
     2401 ggcggagcac gtcgtcgtcc atgcacggca gatcgtcgtg caccagcgag taggcgtgga
     2461 tcagctcgac cgccgcggcc gcgacctcga gccgctcggg gctcgccccc gccacctcgc
     2521 cggcggcgaa ggtaagcaag ggccgcacgc gcttgccgcc ctcgagcacc gcgtagcgca
     2581 tggccacgtg cagccgcgcc ggggcgaggt gggccgcggg caggtgacgg gagagaaccg
     2641 cctccattcg cgcctggatg ccgcccatcc aggccgcgaa gcccgggtca ggtgccgtca
     2701 ccgggctcgg gttccacggc gagcttcttc agcacctcgc cttcgagcac cttgacctgg
     2761 cgctgggcgg cgtcgaggcg ctcctggcag aagcgcgcca gctccaggcc gcgcttgtgc
     2821 gccgccagcg cctgctccag cgagagcttg ccgccttcca tctgcgcgac cagcttttcc
     2881 agctccgcga gcgctttctc gaaggtggcc tcggcggcgg gcttggtcat ggcgcgaact
     2941 tagccgaccg gcgctttacc ggtcaaacct tccctggggt agaatgcgcg ccattttttc
     3001 gccaccccgg gctccgatgt ccgacctcgt ccacgcgcgc cgactgcagc cggcctcctg
     3061 ccagctgccg gtgtcgtggt atttcgaccc cgccgtgttc gccgaggaaa aggcgcgcct
     3121 cttcgacccg gggccgaact acatcggcca cgagctgatg gtgccgcagg tcggcgacta
     3181 ccacacgctg gcgtggatgg accacgccaa ggtgctggta cgcaacgccg gcggcgtcga
     3241 gctcctctcc aacgtctgcc gccaccgcca gtcgctgatg ctcgaaggcc gcgggcggct
     3301 gcagaacatc gtctgcccgc tgcatcgctg gacctacgac ctgaagggcg agctcctcgg
     3361 cgcaccgcac ttccccgaga gcccgtgcgt caagctcgcc tccacgcctc ttacgcgttg
     3421 gaacgggctg ctgtttgccg ggccggcgga tccggcgcgc acgctcgccg gcatggacac
     3481 gtcggactgg gatttctccg gctacgtgct cgagcgcgtg atgatcgacg agtacgacgt
     3541 caactggaag accttcatcg aggtgtacct cgaggtctac cacgtcgacc cgttccatcc
     3601 gggcctgggc aacttcaccg actgcaagca gtggcacgtc aactacgggg aggactggtc
     3661 ggtgcaggtg gcgcaggcga aggcgggcct cggcaagccc ggcacgcccg tgtaccgcaa
     3721 gtggcacgag gcttgcctca agcacctcga cggccgcacg cccaagcagg gcgcgctgtg
     3781 gatggcctgg ttccccggct tcatgctcga gtggtacccg aacgtgctca tcgtcagcca
     3841 gctgatcccg cgctcgccga cgcacaccac caacgtggtc gagttctact accccgagga
     3901 gatcgcgctg ttcgagcgcg agttcgtcga ggcgcagcag gccgcgtatg tcgagaccgc
     3961 gctcgaggac aacgagatct gcctgcgcat ggaccgcggg cggcgcgcgc tctggacgca
     4021 gggacgcgac gacgccggcc cgtaccagtc gccgatggag gacacgatgg tacatttcca
     4081 cgaatggctg cggagaaagc tggggcgcta ggccctctcg tatcgaccga agatctgtcc
     4141 cgccaccccg agtggcgcgt gttcgactgc cggcacgacc tgatgaagcc ggaggccggc
     4201 gagcagctgt accgtgaagg tcacatcccc ggcgcttcgt tcgcgcatct ggatcgcgat
     4261 ctctcgtcgc ccaagaccgg aaagaacggt cggcatccat tgccggatcc cgcgaagttc
     4321 atcgcctggc tgggttcgca gggactgaag aagaccgaca ccgtcgtgtg ctacgacggc
     4381 gggccgggcg cgatggccgc gcgcctgtgg tggatgctgc gctgggtggg gcacgagtcg
     4441 gtctcggtgc tcgacggcgg cgtcgccaag tggaagaagg aaggccgggc ggtcactgcc
     4501 gaggtcgcga agttctcgcc gacgacgtat cccgggaagg cgcgtagcga gatggccgtg
     4561 gacgtcgccc acgtcgagaa atctctgggc gaggagctgg tcctcgatgc gcgcgcgccg
     4621 gcgcgctggc gcggcgagac cgagcccatc gacccggaag ccgggcgcat tcccggatcg
     4681 ctgaatcgct tcaacagcga caacctcacg cccgagggcg tgcacaagcc ggccgagcag
     4741 ctgcgcaggg agttcgaggc cgtgctcggc gggcgcgcgc ccgcggaggt cgtcaactcc
     4801 tgcggctcgg gcgtcgccgc gtgccacaac atcctggcga tggagatcgc ggggttgaag
     4861 gacgcgaagc tctaccccgg ctcctggagc gagtggatcg cggatcccaa gagaccgcgt
     4921 gagcgcggct gattctctcc tcgtccttca cttcgcgatc gtcgttttca tcgtcggcgg
     4981 gctgctcctg acttgggtcg gggcgctcgc gtcctgggcg tgggtgcgca acccctggtt
     5041 tcgctacctg cacctcggag ccatcgcctt cgtcgcgttg gaagcggtca tcggcatgac
     5101 ctgtccgctc accgtgtggg aggacgcgct gcgcggcggc ggaagcggcg agtcgttcgt
     5161 ggggcgctgg gtgcggaccc tgctttacta ccgcgcgccg gagtgggtgt tcacggtgat
     5221 ctatgtggcg tggacggcgg cgacgctcgt cacgctgcgg ttcgtgccac ctcggcgctg
     5281 ataccctcgc gcaagcgcgc cagcgcctcg ggctcgccgc gcacgcgcag gaacgcgccg
     5341 gcgccgtccg cacgctcctc gagcacttcg cagcccgcga agatctggcc gcgcagcttc
     5401 tgcgccgacc agggcaggta gagctcggcc tcgaccaggc gccgctggaa gaacgcgacg
     5461 atcgcctcgc gcaaccgcgc cacgtcgcct tcgcgcttgg cgctcatgac cacgcacccg
     5521 gggtacttct cgcgcagcgc ggcctcgtcg ccgccgcggt cgatcttgtt gaacacgatc
     5581 aggcgcggca cgtccttggc cccgatctcg tcgagcacct cctcggtcac ggcaagctgg
     5641 cgctcgaacc cgggatcgct cgcgtcgacc acgtgcgcga cgagcgaggc ctcgagcgcc
     5701 tcgtcgagcg tcgacttgaa cgaggcgacc agcccgtgcg gcaggttctt gatgaagccg
     5761 accgtgtcgc tcaccagcac gcgcggcacg ctctccggat gcagcgcgcg caccgtggtg
     5821 tcgagcgtgg cgaagagctt gtcggccacc agcgcctcgc tcgccgtaag cgcgcgcatc
     5881 agcgtggact tgcccgcgtt cgtgtagccg acgagcgcca cgcgcgcgag tccctggcgc
     5941 tcctggcgcc gcgcgcgctg cgtccggcgc tcgaggtgca tcgcgtcgag ctctttctgc
     6001 agctcggcga tgcggtcgcg gatcttgcgc cggtcaagct cgccgtgcga ctccccgccg
     6061 ctcctgccgc cggtgccgct gcgcgcccgg tccttcggcc cggcctgctt ggcggcttcg
     6121 cgcatcctcg gccccatgta gcccaggcgc gcgatctcga cctgcgcgcg cgcggcgctg
     6181 gagcgcgcgt ggcggtggaa gatctcgagg atcaccatgg tgcggtccat cacctcgcag
     6241 ccgacctcct tgccgaggtt gaacgcctgc gagggcgaga tctcgtggtc gacgaggacg
     6301 aggtccgcgc cttgctgtac caggtcgcgc agctcctcgc gcttgccgag gccgaagtag
     6361 gcggtggcgt cgaagctggc gcgcttctgg gtgatcgtac tgacgacttc gaagccgagc
     6421 gttttcgcga gctggcgcag ctccgtcagc gaggcttcga attcggcgtc accgacgctg
     6481 ggcaattgca ccgccgcgac gatggcgcgc gggggtttgt gggcgagctc ttgcgtcatg
     6541 ggggacggag actaccagtc aaagccgcat ttcggcagat ttcccggctt gtcagtgcac
     6601 tcgtgccgcc caggaaaaca acgcgctgaa cacggcatcc cgcacctcgc gccgcgacag
     6661 cacgagatcg tgcagcccgc ccggaaacgc gagcacggac gcctgcggcc ccaaagtcct
     6721 cccccacttg gcgacgtggc gccagtcgag cacgatgtcg gcctcgtccg aatgcatggt
     6781 cagcaccggg cactcgacct ccaacccggc atgcaccttg tgctggccgg caagcacggc
     6841 accgatccag ccgaagtacg ctggaaagcc gtgcaagggc ttcagcttca ggtcgaagtc
     6901 ccactcgccg tcgaagtcgc ggtggatgct gcggatgtag acgggcttca gcgccttggg
     6961 atcattgaga aacggaaacg cctgccccgt gagcgccgcc atgccgagct gcgcgcgctt
     7021 caaaggcgtc accttccagt cgaagaacgg gctgttcagc catagcgaag taatctgctc
     7081 gcgccgcgcg ccgcggtgcg cgtaaagcgc gcacacgagc ccgcccgtcg aatgtccggc
     7141 aagcagcacc gggccgccga tctcgtcgat cgcggccgtg atgtcgacgt cgtactccgc
     7201 catgtccttg cagtagcacg gatgctggtg ctcgagccac gagcgtccgt gcttgcgcaa
     7261 atcgagcgcg tagaacgcgt agccctcgcc ggcgaagcgc tcggccatgt ggcgctggaa
     7321 aaaataatcg acgaagccgt gcacgtagag caccgccccg cgtggcgcat cgcccgccgg
     7381 caggcgcacg agcgtcgcca ccacgcgccc atcgtagtcg tcgggaaacc cgaaggtctt
     7441 cgcctcgaaa cccggcagca gccggtccgg ttgccagtca ctcatggcgc gaaagcgccc
     7501 aggccacgtg ctcgcgcacc agggcggacg ggtggtccga gcgagcgcgc agcgcgccga
     7561 gcacttcggg cgtggtcggc gcgttgccca ggcccacggc gaggttgcgc agccaccgtt
     7621 cgtagccgat gcgccggatc ggcgagcccg ccatgcgctc gtcgaacgcc tgctcgctcc
     7681 aggcgaagag ttccaccagc gacgcgcgat cgaggccgtt gcgcaccgcg aagtccggct
     7741 cccgcgtcgg ctgcgcgaac gaattccatg ggcacaccgc ctggcaatcg tcgcagccgt
     7801 agacgcggtt gccgatcagc ggccgaaact cctccggaat gcagcctttc agctcgatgg
     7861 tgaggtacga gatgcagcgc cgcgcgtcga gctggtaggg cccggtgatc gcctgcgtcg
     7921 ggcagacgtc gatgcacttc gtgcacgtgc cgcaatgctc gcccgcgggg gcgtccgccg
     7981 gcagcggcag gctcgtatac acctcgccca gaaagaacca cgatcccgct tcgcgcgaca
     8041 gcagaagcgt gtgcttgccg cgccagccga tgccggattt tgctgcgagc gccacctcca
     8101 tcaccggcgc cgagtcggcg aacacgcgat gcccgaaagg gccagcctcc gcctcgatgc
     8161 gctcgcacag cttctgcagc cgcccgcgca gcaccttgtg gtagtcgcgc ccgcgcgcat
     8221 agcgcgagac gcaggcacgc agcgggtcgt cgtcttcctc gtccagcgtc gccgcatagt
     8281 ccatgcggca gctgatgacg cgcacggtgc ccggcaccag ctccgcgggc cgcgcgcgga
     8341 gcgcgccgtg gcgcgccata taatccatct cgccgtgcca gcccctgccg agccactcgc
     8401 cgaggcggtt ttccgcggcg gcgaggtcgg tgtccgcgat ccccaccgcc tgaaagccga
     8461 gctccccgcc ccactgcttg attcgcgccg caagcgcgcc gtaatccatg ggtcccgagt
     8521 ctagcctcga gctcgccgac gaagcggcga cgctcggcct aggcgcggcg ctcgccgccg
     8581 gcatcgcgcc ggggcgcgtg ctgcacctct cgggcgagct cggatccggc aagaccaccc
     8641 tcgttcgggg actgttgcgt gaactgggtc acagcggccg ggtgaaaagc cccacctacc
     8701 ccctggttga actttacgtc gtttcaagat taaacttgta tcactttgat ttttatcgtt
     8761 tcaaagatcg gagcgaatgg caggattctg gcttcaggga ttacttcaac gcgcaagccg
     8821 cgtgcatcgt cgaatggccg gagcgggcgg gcggactgct gccgcccccg gacctcgcgg
     8881 tgcggctgga aatcgtcccg ggcgcgcgcc gcgcccggct gagcgcgggc acgccggccg
     8941 gcgcggcctg gctggcgcgc ttgcggccgc gctcctcctc ggctacctag ccgccgccca
     9001 ggcgcaggtc atcgccacgc gcatctggcc ggcgcgcgac tacacccgcg tcaccttcga
     9061 gtcgcgtcag gcgctcgact acagcatgtt cgcgctgaag gacccccaac gcctggtgct
     9121 cgatctcaac gccgaggacg cggacggcct gatcgccgcg ctgcagtcca aggtcgccgc
     9181 cgaggatccc tacgtcgcgg tgctgcgcgt gggcgccaac cgcccgggcg tgacgcgcgt
     9241 ggtgcttgac ctcaaggcgg aagtgcggcc gcaggtgttt gcgctgaagc ccatcgccga
     9301 atacggccac cgcctggtgc tcgaccttta tccgctggtg ccgcccgatc cgctggcggc
     9361 gctcctggag ctgaacgggc agctcgccgt gcccggggcg cgcggcggcg acaccacgcc
     9421 gcgctcggtg gtgcgcctcg ctaccgtagt ggtggatgcc gggcatggcg gcgaggatcc
     9481 gggcgcgcgc gggcgccgcg gcgcgaagga aaaggacatc acgctgctca tcgccaggcg
     9541 cctgaaggcg ctgatcgacg ccgagcccgg catgcgcgcc gtgctgacgc gcgacggcga
     9601 ctattacctg tcgctgcagg cgcgtgtgga gaaggcgcga gcggtgaaag cggatctctt
     9661 ggtgtcggtg cacgccgatg ccttcacccg cccgcacgcg cgcgggtctt ccgtgttcac
     9721 gctgtcgcag cggccgacct cggcggccgc ggcgtggctg gccaagcagg agaacgactc
     9781 cgacctgatc ggcggcgtcg gcctggacgc gcgcgacccc tacctcaagc aggtgctcgt
     9841 ggacctgtcg cagaccgcga ccaacgatca cagcatgaag ctcgccggcg cggtgctctc
     9901 ggagctcggc cagatcaaca cgctgcacaa ggcgcaggtc gagcgcgccg gcttcgccgt
     9961 gctcaaggcc cccgacgtgc cctcgatcct ggtcgagacc gcgttcatct ccaatccgca
    10021 ggaggagcgc cggctgcagg acgagcgcta ccaggacaag atggcgcgcg ccatgctggc
    10081 ggggatcaag cgctacttca gcctgcaccc gccgcgcccg cagggtccgc tggcgcagaa
    10141 atgagtcagg gcgtggcgcg ccgcgcgcgg gcgcgcagga actccaccgc gatcggcagc
    10201 accgagatca ccacgatcac cacgatcacc gcggcgaggt tctccttcac cgccggaatg
    10261 ttgccgaacc agtagccggc gaggctgagc gatacgaccc acagcagcgc gccggcgacg
    10321 ctgaacgcca ggaagcgcgc gtacggcatg gcgccgatgc cggcgacgaa cggcacgtag
    10381 gtgcgcacga tcggcacgaa gcgcgcgatg atgatcgcct tgccgccgtg gcgctcatag
    10441 aaggcgtgcg tgcgctcgag gtggcggcgg ttgaaccagc gcgaatcctc cgcgcggaac
    10501 accttcggcc cggcccagcg cccgatccag tagttgacgt tgtcgcccga gagcgccgcg
    10561 gcgacgagca gcgccatcac cagcacgatg tccatgccgc cggcggcagc gagcgtgccg
    10621 acgacgaaca gcagcgagtc cccggggagg aacggcgtca ccaccagccc cgtctcgcag
    10681 aagacgatca cgaacagcag cgcatacacc cacgcgccgt gctcggcgat gaacgcggcg
    10741 agatgtgcat ccagatgcag cgcgaaatcc cacagggcgg cggcgagctc catgacccga
    10801 ttctatatac tccccgcatg agcgctatcc gcgtcatgcc cgagctgctc gtcagccaga
    10861 tcgcggccgg cgaagtggtc gagcggccgg cctcggtgct gaaggagctg ctcgagaacg
    10921 gcctcgacgc cggcgcgacc gagctgcagg tgacgctgga tgccggcggc gcgaagcgcg
    10981 tgcaggtcga cgacgacggg gccggcatcg cccaggacga cctcgcgctg gcgctcgcgc
    11041 gccacgccac cagcaagatc ggctcgctcg acgacctcga gtcggtcggc acgctcggct
    11101 ttcgtggcga ggcgctggca tccatcgccg ccgtggcgcg cgtggaaatc gtctcgcgcg
    11161 caaggggggc ggcgcatgca tggtcggtcg cggctgaggg cggggcgccg gctgcgccac
    11221 gccccgcagc gcgcgcgccg ggcaccactg tctgcgtcgg cgacctgtac ttcaacaccc
    11281 cggcacggcg caagttcctg cgcaccgagg ccaccgagtt cggccactgc gacgacgtgt
    11341 tccggcgcat cgcgctcgcg cggccggatc tcgccttcac gctgcggcac aacggtcgcg
    11401 tttccctgca cttgaggccg caggatctgc cggcgcgcgc cgccgcgctg ctcggcgcgg
    11461 agttcgccgc ggccgcggtg cccgtcgacg cgcaggccgg cgcgctgcgc ctgcacggcc
    11521 tggcggggac gccgcagtcg gcgcgcagcc gcgccg
//
LOCUS       DBNHKE010000186         6216 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_32818, whole genome shotgun sequence.
ACCESSION   DBNHKE010000186 DBNHKE010000000
VERSION     DBNHKE010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_32818"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1450)
                     /locus_tag="AC16O9_12760"
     CDS             complement(167..1450)
                     /locus_tag="AC16O9_12760"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6517525.1"
                     /translation="MYIVGLVVLVLLVLSVPIGIALSAASAAYILIDPLLEPTVIFRA
                     FFSFITKYSLMAIPLFIFAGFLMERTGLVRLLFQFSDALVGWMPGGFGVATVVACVLF
                     GAISGSSVAMAAAMSVIAIPEMRSRGYPDWFSAGLVATAGGIAMLIPPSIILILYGIV
                     TETSVVRLFFAGVVPGLLLATGNILSVVVIAWFLKLPRGKFEPRRVWREFLRAAPALG
                     MPVVILGGLYGGLFTPTEAAAAACGYALLYGLLSDTRAFLKHLLPTASRALNLTAVVL
                     FLVGCVGVFQFLAANMYWPQRITETVTGWGLTPLTFIFAFMGVLMMLGMFVDGVAMVL
                     LTVPVVFPVAYALKIDPVHLGIMLALTVEMSVVTPPVGLNLFAVSGTSKIPVTQVLRG
                     SVPFFLSDFLVLILVIFFPALATWLPNLLVIDVFK"
     gene            complement(1468..2019)
                     /locus_tag="AC16O9_12765"
     CDS             complement(1468..2019)
                     /locus_tag="AC16O9_12765"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6517526.1"
                     /translation="MERAYAVWRAFQDRFLTHAAALILLACTLLALLEVVRRYVFGVS
                     YEWQQDAVTFFIVSAVFLYFGISQRHNEHLNVVLLLEVLQAAGPRARQAAEIIKLVAL
                     VLSLIFMIAVVWWGIPEVEDAMKYASRTESLAFPMAPFLWALIVGFAFMGITMVFQIY
                     FEIQKLRGREVPEEAPILDRMPD"
     gene            complement(2028..3167)
                     /locus_tag="AC16O9_12770"
     CDS             complement(2028..3167)
                     /locus_tag="AC16O9_12770"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6517527.1"
                     /translation="MRQKLLRRLPTAAAMAMASASLIAGLGAAQIAHAQDKAKPVVMR
                     FVSDFPPPPHPAGLAMKHFADRLPQVIPGSEARLYFAGALYTVPEAFEAMRQGNLEMT
                     WMQIGKTAPVDAWMMSLVGPGILTTVGAVDSLEKTQTYQMLVKRLESQQRIKVFGAGH
                     MSFGMGVGGKVRYAKPDDFTGKKVRSMGPVENASLESWKANPVVMAFGEVPSALESGV
                     IDGLMTSIGGWLAVKEQAPFYTMGGAGAFIGDYYMVSSSRRWWDRLSEAQRTALEGLI
                     KETITVQKELNWCVDKMTYDKYGTKDPSKPGVYWMSAQEVDAMVTAMGDKPTRYVKSK
                     TPEAANKWVDLFVQEGRELSKKNPTGSSWIEKVDCSKHASKIVIK"
     gene            complement(3283..4101)
                     /locus_tag="AC16O9_12775"
     CDS             complement(3283..4101)
                     /locus_tag="AC16O9_12775"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6517528.1"
                     /translation="MDVRPIAGALGAEIRGLDLGQPLDAGTIAAIRAAWLEHLVIFFH
                     GQTLTPAQQLAFARAFGEPMEYPQLKGLPECPLITPVVKLEHERTNFGGVWHSDTTYL
                     ERPPMASMLYALEVPPRGGDTLFANQYLAYETLSEGLQRTLDGLVGVNTSTKAAASKT
                     REDRMRAAGEELKVLAGEHPVVRTHPETGRKALYVNIGHTERFRGWTEEESRPLLEYL
                     FAHQVRPELTCRFRWAPGSLAFWDNRCAQHNPVNDYHGYRRVLHRVTLAGDAPR"
     gene            complement(4120..4425)
                     /locus_tag="AC16O9_12780"
     CDS             complement(4120..4425)
                     /locus_tag="AC16O9_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517529.1"
                     /translation="MTRLYSLTEAGRRAFETQSSKVPLDSRRVLGLIKPDTDPHKVCA
                     RLGWSEAAVIEILEELEFGGLVRSIGASASPDEPDLDFTNSLKVADIQAALRARDKK"
     gene            4515..4970
                     /locus_tag="AC16O9_12785"
     CDS             4515..4970
                     /locus_tag="AC16O9_12785"
                     /inference="COORDINATES: protein motif:HMM:NF023010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HIR6517530.1"
                     /translation="MIQTRHLFTLTAEVPKIVDMGATPAGQRRIAHVAGGAFKGERLS
                     GTIHPAPGGDWILVRPDGATVLDVRITLETDDRQLIYMTYRGVRHGPAEVMARLAKGE
                     AVDPASYYFRTTPVFETASPKYDWLNRIVSVATGRREASGPVYEVYEVL"
     gene            complement(4955..5983)
                     /locus_tag="AC16O9_12790"
     CDS             complement(4955..5983)
                     /locus_tag="AC16O9_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6517531.1"
                     /translation="MMRLPWFEWRAPRSLAEAANILAGEGPQAMLIAGGTDLVPNMKR
                     RHQSPKTLVSLRGVSELRKSNGALGAGLTLTALVESPQVRERYRALWQAAAQIATPHL
                     RNMGTLGGNLCLDTRCTYYNQSHEWRQAIDFCLKKDGETCWVATASKRCVAVSSTDGA
                     PALIALDAKVKLVSAGGERELALADLYRNDGIDYLTRRPDEILTEVAIPEGWTSSYWK
                     LRRRGAFDFPVLGVAAAVKLAQDGTVEAARVALGAAASRPFLVDKAGEFLNGQRLSDE
                     VIAEASSLVASRAKPMDNTDLDLYWRKDVASSFAGYALREVRGEDMRETRLRIARQTL
                     DPATQSTS"
     gene            complement(5980..>6216)
                     /locus_tag="AC16O9_12795"
     CDS             complement(5980..>6216)
                     /locus_tag="AC16O9_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517532.1"
                     /translation="PVMPALANAVHDAVGVRVDEIPVTPDKILRALEKKAKGETPRVG
                     PESFPAVPYPKPTFVLPPWEGGDGNETKEGRKAA"
BASE COUNT         1024 a         2170 c         2057 g          965 t
ORIGIN      
        1 gatcgttcta tggccagggt ggacttcagg gtaacccacc caggcagaca aagggtaatt
       61 atcttttcgc ggcccgcccg tacaaccgtc aggccgcgca agcgcgaggg aataaaaccg
      121 ccgccgccgt gtgcggcccg gcgcgccggg ccagcgacgc ccgcgcctac ttgaagacgt
      181 cgatcaccag caggttcggc agccaggtcg ccagcgccgg gaagaagatc accaggatca
      241 gcacgaggaa atcgctgagg aagaacggca ccgacccacg cagcacctgc gtgaccggaa
      301 tcttcgacgt cccgctcacc gcgaacaggt tcaggcccac gggcggcgtc accacgctca
      361 tctccaccgt cagcgccagc atgatgccca gatgcaccgg gtcgatcttc agcgcatagg
      421 ccaccgggaa caccaccggg accgtgagca gcaccatggc gacgccgtcc acgaacatgc
      481 cgagcatcat cagcacgccc atgaaggcga agatgaaggt gagcggcgtg aggccccagc
      541 cggtgaccgt ctcggtgatc ctctgcggcc agtacatgtt ggcggcgaga aactggaaca
      601 cgcccacgca gccgaccagg aacagcacca ccgccgtgag attcagggcg cggctcgccg
      661 tcggcagcag atgcttcagg aacgcgcgcg tgtcgctgag caggccgtac agcagcgcgt
      721 agccgcaggc ggcggcggcc gcctcggtcg gggtgaacag ccccccgtag aggccgccga
      781 gaatgacgac cggcatgccg agcgccggcg cggcgcgcag gaactcgcgc cagacgcgcc
      841 tcggctcgaa cttgcctcgc ggcagcttga ggaaccacgc gatgaccacc acgctgagga
      901 tgttgccggt ggcgagcagc aggcccggca ccacgccggc gaagaacagc cgcaccaccg
      961 aagtctcggt cacgatgccg tacaggatca ggatgatgct cggcgggatc agcatggcga
     1021 tgccgcccgc ggtggccacc agcccggccg agaaccagtc cggatagccg cggctgcgca
     1081 tctccggaat ggcgatcacg ctcatcgccg ccgccatggc cacgctcgag cccgagatcg
     1141 cgccaaagag cacgcaggcg accacggtgg cgacgccgaa cccccccggc atccagccca
     1201 ccagcgcatc ggagaactgg aacagcagcc gcaccagccc cgtgcgctcc atcaggaagc
     1261 cggcgaagat gaacagcggg atcgccatca gcgagtactt cgtgatgaag ctgaagaacg
     1321 cgcggaagat gaccgtgggc tcgagcagcg ggtcgatcag gatgtaggcc gcgctcgccg
     1381 cgctgagcgc aatgccgatc ggcacgctga gcaccagcag gaccaggacg acgaggccga
     1441 cgatatacat ctagggcagg cctgggctca gtcgggcatg cggtccagga tcggcgcctc
     1501 ctccggcacc tcccgcccgc gcagcttctg gatctcgaaa tagatctgga acaccatggt
     1561 gatgcccatg aaggcgaacc ccacgatcag cgcccacagg aacggcgcca tgggaaacgc
     1621 caggctctcg gtgcggctcg cgtacttcat cgcgtcttcc acctcgggaa tgccccacca
     1681 caccacggcg atcatgaaga tgagcgacag gacgagcgcc accagcttga tgatttccgc
     1741 ggcctgccgc gccctcgggc cggcagcctg caggacctcc agcagcagca ccacgttgag
     1801 gtgctcgttg tggcgctgcg agatgccgaa gtacaggaac accgcgctca cgatgaaaaa
     1861 cgtcaccgcg tcctgctgcc actcgtacga cacgccgaac acgtagcgcc gcaccacctc
     1921 gagcagcgcg agcagcgtgc aggcgagcag gatgagcgcc gcggcgtgcg tcaggaaccg
     1981 gtcctgaaac gcacgccaga cggcgtaggc tctttccacc taggcgctta cttgatgacg
     2041 atcttggagg cgtgcttgga gcagtccacc ttctcgatcc aggacgatcc ggtcggattc
     2101 ttcttcgaca gctcgcggcc ttcctgcacg aacaggtcga cccacttgtt cgccgcctcg
     2161 ggcgtcttgc tcttcacgta gcgcgtcggc ttgtcgccca tcgccgtcac catcgcgtcg
     2221 acttcctgcg cgctcatcca gtacacgccc ggcttggaag gatccttggt gccgtacttg
     2281 tcgtaggtca tcttgtcgac gcaccagttg agctccttct gcaccgtgat ggtctccttg
     2341 atgaggcctt cgagcgcggt gcgctgcgcc tcggagaggc ggtcccacca gcgccggctg
     2401 gagctgacca tgtagtaatc gccgatgaac gcgcccgcgc cgcccatcgt gtagaacggc
     2461 gcctgctcct tgacggcaag ccacccgccg atcgaggtca tcaggccgtc gatgacgccg
     2521 gactcgagcg cgctcggcac ctcgccgaag gccatcacga ccgggttcgc cttccacgat
     2581 tcgagggagg cgttctccac cgggcccatg ctgcgcacct tcttgccggt gaaatcgtcg
     2641 ggcttggcgt agcggacctt gccgcccacg cccatgccga agctcatgtg cccggcgccg
     2701 aacaccttga tgcgctgctg cgactccagc cgcttcacca gcatctggta ggtctgggtc
     2761 ttctccaggc tgtcgacggc gccgaccgtg gtcaggatcc ccgggccgac caacgacatc
     2821 atccaggcgt cgaccggcgc ggtcttgccg atctgcatcc aggtcatctc caggttgccc
     2881 tggcgcatcg cttcgaacgc ctcgggcacc gtgtacaggg cgccggcgaa gtacaggcgc
     2941 gcctcgctgc cgggaatcac ctgcggcagg cggtcggcga aatgcttcat cgccaggccc
     3001 gccgggtgcg gcggcggcgg gaaatcggaa acgaagcgca tcaccaccgg cttcgccttg
     3061 tcctgcgcgt gcgcgatctg cgccgcgccc aaaccggcga tcaggctcgc cgatgccatt
     3121 gccatcgcgg cggccgtcgg cagccggcgc agcagtttct gtctcatggg tactctcctc
     3181 cagctttcat gatgtgatcg gttcagtgtg cggcgccacc gtatctcacc gcccccccag
     3241 ggtcaaggtt ccgggtcaag atggtgcgat gcggcacgag ggtcagcgcg gcgcgtcgcc
     3301 ggccagggta acgcggtgca agacgcgccg gtagccatgg tagtcgttga ccgggttgtg
     3361 ctgcgcgcag cggttgtccc agaaggcgag cgacccgggc gcccagcgga agcggcaggt
     3421 gagctcgggg cgcacctggt gcgcgaagag gtattccagg agcgggcggc tctcctcctc
     3481 cgtccagccc ctgaagcgct cggtatgccc gatgttcacg tacagcgcct tgcggccggt
     3541 ttccgggtgc gtgcgcacca ccgggtgctc gcccgcgagc accttgagct cctcgccggc
     3601 cgcgcgcatc cggtcctcgc gcgtcttcga cgcggcggcc ttggtcgagg tgttgacgcc
     3661 gaccagcccg tccagggtgc gctgcaggcc ctccgacagc gtctcgtacg cgaggtactg
     3721 gttggcgaac agcgtatcgc cgccgcgcgg cggcacttcc agcgcataga gcatgctggc
     3781 catcggcggg cgctcgagat acgtggtgtc cgaatgccac acgccgccga agttcgtgcg
     3841 ctcgtgctcg agcttgacca ccggcgtgat cagcgggcat tcgggcaggc ctttgagttg
     3901 cggatactcc atcggctcgc cgaaggcgcg cgcgaacgca agctgctgcg ccggcgtgag
     3961 ggtctggccg tggaagaaga tcaccaggtg ctcgagccag gcggcgcgga tcgcggcgat
     4021 cgtcccggca tcgagcggtt gccccaggtc caggccgcgg atctcggcgc cgagcgcgcc
     4081 ggcgatgggg cggacatcca tgccgcggat gttacaggcc tacttcttgt ctcgcgcgcg
     4141 cagcgctgcc tggatgtcgg caaccttgag gctgttggtg aaatccagat cgggctcgtc
     4201 cgggctcgcg ctggcgccga tcgacctgac caggccgccg aactccagtt cctcgaggat
     4261 ctcgatcacc gcggcctcgg accagccgag acgggcgcag accttatgcg ggtcggtgtc
     4321 cggcttgatc aggccgagca cccgccggga gtccagaggt accttgctgc tctgggtttc
     4381 gaaggccctg cggccggcct cggtcaggct gtagaggcgc gtcatacggc ccccattgtt
     4441 cctccgtcgc gcggcacaat gcaagggtgc ggcgccgccg caacgcattc tgacaattgc
     4501 aaggagctcg atgcatgatc cagacacgcc acctcttcac cctcaccgcc gaggtcccga
     4561 agatcgtcga catgggcgcg acccccgccg gccagcgccg catcgcccac gtcgccgggg
     4621 gcgccttcaa gggcgagcgc ctgagcggaa cgatccaccc cgcgcccggc ggcgactgga
     4681 tcctggtgcg cccggacggc gcgacggttc tcgacgtgcg catcaccctc gagaccgacg
     4741 accggcagct gatctacatg acctaccgcg gcgtgcgcca cgggccggcc gaggtgatgg
     4801 cgcgcctggc caagggcgag gcggtcgatc cggcgtccta ttacttccgc accaccccgg
     4861 tgttcgagac ggcctcgccg aagtacgact ggctgaaccg catcgtgtcc gtcgcgaccg
     4921 gccggcgcga ggcgagcggg ccggtgtacg aggtctacga ggtgctctga gtggcgggat
     4981 cgagcgtctg gcgcgcgatg cgcaggcgcg tctcgcgcat gtcctcgccg cgcacctcgc
     5041 gcagtgcgta gccggcgaag ctcgacgcga cgtccttgcg ccagtacagg tcgaggtcgg
     5101 tgttgtccat gggcttggcg cgcgaggcaa cgaggctcga ggcttccgcg atcacctcgt
     5161 cgctgaggcg ctggccattc aggaactcgc ccgccttgtc caccaggaac gggcgcgagg
     5221 cggcggcgcc caacgcgacg cgcgccgctt ccacggtgcc gtcttgcgcc agcttcaccg
     5281 ctgccgcgac gccgagcacc gggaagtcga acgcgccgcg ccggcgcagc ttccagtagc
     5341 tgctcgtcca gccctccggg atcgccacct cggtgaggat ctcgtcgggc ctgcgcgtca
     5401 ggtagtcgat gccgtcgttc ctgtagagat cggcgagcgc gagctcgcgt tcgcctccag
     5461 ccgaaaccag cttcaccttc gcgtcgagcg cgatcagcgc gggcgcgccg tcggtggagg
     5521 acaccgcgac gcagcgcttg ctcgcggtgg cgacccagca ggtctcgccg tccttcttca
     5581 ggcagaagtc gatcgcctgg cgccactcgt ggctctggtt gtagtacgtg cagcgcgtat
     5641 cgaggcacag gttgccgccc agcgtgccca tgttcctcag gtgcggggtc gcgatctgcg
     5701 cggcggcctg ccagagcgcg cggtagcgct cgcgcacctg cggcgactcg accagtgcgg
     5761 tgagcgtgag gcccgcgccg agcgcaccgt tcgacttgcg caactcggac acgccgcgca
     5821 gcgagaccag cgtcttcggc gactggtggc ggcgcttcat gttgggcacg aggtcggtgc
     5881 cgcccgcgat cagcatggcc tgcggccctt cgcccgcgag gatgttcgcc gcctccgcga
     5941 gcgagcgcgg cgcgcgccac tcgaaccagg gcaggcgcat catgccgctt tcctgccttc
     6001 cttggtctcg ttgccgtcgc cgccttccca cggcggcagc acgaacgtcg gcttcggata
     6061 cggcaccgcc ggaaacgatt cgggacccac gcgcggggtt tcgcccttcg cctttttctc
     6121 cagcgcgcgc aggatcttgt cgggcgtgac cggaatctcg tcgacgcgca cgcccaccgc
     6181 gtcgtggacc gcgttggcga gcgccggcat caccgg
//
LOCUS       DBNHKE010000187         3796 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1461259, whole genome shotgun sequence.
ACCESSION   DBNHKE010000187 DBNHKE010000000
VERSION     DBNHKE010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1461259"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16O9_12800"
     CDS             complement(<1..240)
                     /locus_tag="AC16O9_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - obsolete D-gluconate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HIR6517533.1"
                     /translation="MIVLVMGVSGSGKSTVGALLAERLGWEFLDADDFHPPANVEKMA
                     AGKPLEDADRWPWLERLNRELRRRRNAVLACSALKA"
     gene            complement(237..1169)
                     /gene="gnd"
                     /locus_tag="AC16O9_12805"
     CDS             complement(237..1169)
                     /gene="gnd"
                     /locus_tag="AC16O9_12805"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR6517534.1"
                     /translation="MSASHGGGMDIGIVGLGRMGGNMARRLARGGARVLAYDRERAAR
                     AALAGEPGVTVVEGLAALCAQLEGERVILLSLPEGAATEDTLDALRPLLSAGDLLVDG
                     GNAYYRDSMRRALELSQEGLRYVDAGVSGGVHGLANGYCLMLGGTPSSIEIFAPYARA
                     LAPAAERGWLHCGPAGAGHYAKMIHNGIEYGMMQALAEGFALIAAREDFGFDAARLAE
                     TWRHGSVIRSWLLDLSAEALARDGALEGVAPVVADSGEGRWTALEAVELGVPTPVITS
                     ALMARFSSQGNADYASRLLALMRAGFGGHAITKP"
     gene            complement(1166..2050)
                     /gene="miaA"
                     /locus_tag="AC16O9_12810"
     CDS             complement(1166..2050)
                     /gene="miaA"
                     /locus_tag="AC16O9_12810"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR6517535.1"
                     /translation="MTRTAYALLGPTASGKSRLALELAARHPVEIVSLDSAQVYRGMD
                     IGTAKPSAEERARVRHHLVDLIDPTERYSAGRFRADCVGTIEEIFDRGRIPLLVGGTM
                     LYYKALAHGIDALPAAEPGLRLEIDQRAARHGWPALHAELARIDPATAARVSPNDAQR
                     IQRALEVWELTRRPLSALQTAAAPALPFRLRAYALVPESRGELHRRIAERFDRMLAAG
                     LVEEVANLRRRHTLHGGLPSMRCVGYRQVWNYLEGEYDREVLRDRGIAATRQLAKRQL
                     TWLRGLPGIEPASALTLE"
     gene            complement(2063..2809)
                     /locus_tag="AC16O9_12815"
     CDS             complement(2063..2809)
                     /locus_tag="AC16O9_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6517536.1"
                     /translation="MSGVLMITGGGRGIGAATARLAAQRGYAVCINYLRDRDAAEALT
                     QEIAGGDGRAIAVAGDVALEKDVLHLFQECDRSLGPLTALVNNAGIVDRQSRVEEMDA
                     ARLQRMFSINVVGAFLCAREAIRRMSTRHGGKGGAIVNVSSAAARLGSPGEYVDYAAS
                     KGAIDTMTLGLAKELAADGIRVNAVRPGFVHTDIHASGGQPNRIERLRDGIPMKRGGQ
                     PDEVARAVLWLLSDEASYATGAILDVSGGR"
     gene            complement(2806..>3796)
                     /locus_tag="AC16O9_12820"
     CDS             complement(2806..>3796)
                     /locus_tag="AC16O9_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair MutL family protein"
                     /protein_id="HIR6517537.1"
                     /translation="PQSARSRADAQYFFVKGRYVRDRLLVHAVREAYRDTLHGERQPA
                     FVLALELDPRGVDVNVHPAKTEVRFRDSRGVHQFVLHALQRALAPSASDAPVSYASVS
                     APGPAIQAALAMREPTPGYRPAPAASPLPAAQKAPPLGYALAQLHGVYILAQNEAGLV
                     LVDMHAAHERILMEKMKLGLDAGGIERQSLLVPAVFRAEALDIATAEENRGALETLGL
                     ELSVAGPNELAVRAAPALLAGGDVPVLVRSLLAELREFGAAQVLAARQNELLATMACH
                     AAVRANRQLSVPEMNALLREMEQTERSGSCNHGRPTWYQLTLGDLDKLFLRGR"
BASE COUNT          536 a         1399 c         1344 g          517 t
ORIGIN      
        1 ggccttgagc gcggagcacg cgagcaccgc gttcctgcgc cggcgcagct cccggttcag
       61 ccgctcgagc cacggccagc ggtcggcgtc ctcgagcggc ttccccgcag ccattttctc
      121 cacgttcgcg ggcgggtgga aatcgtctgc gtcaaggaac tcccagccca ggcgctcggc
      181 gagcagcgcg ccgacggtgc tcttgcccga gcccgacacg cccatcacga ggacgatcac
      241 ggcttcgtga tcgcgtgccc gccgaagccg gcgcgcatca gcgcgagaag ccggctcgca
      301 taatcggcgt tgccctggct ggaaaagcgc gccatcagcg ccgaggtgat gaccggagtc
      361 ggcacgccga gctccaccgc ctcgagcgcc gtccagcgcc cttcgcccga atccgccacc
      421 accggcgcca cgccttcgag cgcgccatcg cgcgccagcg cctcggcgct caggtcgagc
      481 agccatgagc ggatcacgct gccgtggcgc caggtctcgg cgagccgcgc ggcgtcgaag
      541 ccgaaatcct cgcgcgccgc gatcagcgcg aagccctcgg caagggcctg catcatcccg
      601 tactcgatgc cgttgtggat catcttggca tagtggccgg cacccgccgg cccgcagtgc
      661 agccagccgc gctcggccgc gggcgccagg gcgcgcgcgt agggcgcgaa gatctcgatc
      721 gaggacggcg tgccgccgag catcaggcaa tagccgtttg ccagtccgtg gacgccgccc
      781 gagacgccgg cgtcgacgta gcgcaagccc tcctgcgaca gctcgagcgc tcggcgcatt
      841 gagtcccggt agtaggcgtt gccgccgtcg acgagcaggt cgcctgccga tagcagcggc
      901 cgcagcgcgt cgagggtgtc ttcggttgcc gcgccctccg gcagcgagag cagaatcaca
      961 cgctcgccct ctagctgcgc gcaaagcgcg gcgaggccct cgacgacagt gacgccgggc
     1021 tcgccggcga gcgccgcgcg cgccgcgcgc tcgcggtcgt aggcgagcac gcgcgcgccg
     1081 ccgcgcgcga gccgccgcgc catgttgccg cccatcctgc ccagcccgac gatgccgatg
     1141 tccatgccgc ctccgtgcga tgcgctcatt ctagagtcag cgccgacgcg ggctcgatcc
     1201 ctggcaggcc gcgcagccag gtgagctggc gcttggcgag ctggcgtgtc gccgcgatgc
     1261 ccctgtcgcg cagcacctcg cggtcgtact cgccctcgag gtagttccag acctggcgat
     1321 agccgacgca gcgcatgctc ggcaggccgc catgcagtgt gtggcgccga cgcaggttcg
     1381 cgacctcctc caccaacccg gcggcgagca tgcgatcgaa gcgctcggcg atgcgccggt
     1441 gcaactcgcc gcggctctcc ggcaccagcg cgtaggcgcg caaccgaaag ggcagcgccg
     1501 gcgctgccgc cgtctgcagc gccgacagcg gccggcgcgt cagctcccag acctccagcg
     1561 cgcgctggat gcgctgcgcg tcgttgggcg aaacgcgcgc tgcggtggcg gggtcgatcc
     1621 gggcgagctc ggcgtgcagg gcaggccagc cgtggcgcgc ggcgcgctgg tcgatctcca
     1681 ggcgcaggcc cggctcggcc gcgggcaggg catcgatccc gtgcgccaga gccttgtagt
     1741 agagcatcgt gccccccacg agcagcggga tcctgccgcg atcgaaaatt tcctcgatcg
     1801 tgccgacgca gtcggcgcga aaccggcccg cggaataccg ctccgtcggg tcaatgagat
     1861 ccaccaagtg gtgccgcaca cgggcgcgct cttccgccga cggtttcgcc gtgccgatgt
     1921 ccatgccgcg gtacacctgg gccgaatcga ggctcacgat ctcgaccgga tggcgcgcag
     1981 cgagctcgag cgccaggcgt gacttgccgc tggcggtggg gccgagcagc gcgtacgcgg
     2041 tccgggtcac ggcgcgcgac ttctagcgcc cgccgctgac gtccaggatc gcgcccgttg
     2101 cgtaggacgc ctcgtccgac agcagccaca gcacggcgcg cgcgacctca tccggctgtc
     2161 caccgcgctt catcgggata ccgtcgcgca agcgctcgat gcggttcggc tggccaccgg
     2221 aggcgtggat gtcggtgtgc acgaagcccg ggcgcacggc attgacccgg atgccatccg
     2281 ccgcgagctc cttggcgagg cccagcgtca tggtgtcgat cgcgcctttg gacgccgcgt
     2341 agtccacgta ctcgcccggc gagcccaggc gcgccgcggc cgaggagacg ttgacgatgg
     2401 cgccgccctt gccgccgtgc cgcgtggaca tgcgccggat cgcctcgcgc gcgcacagga
     2461 acgcgccgac gacgttgatg gagaacatgc gctgcaggcg cgcggcgtcc atctcttcca
     2521 cgcgcgactg ccgatccacg atgccggcgt tgttgacgag ggcggtgagc ggcccgagcg
     2581 agcgatcgca ctcctggaac aggtgcagga cgtccttttc gagagcgacg tcgccggcaa
     2641 ccgcgatcgc ccggccgtcg ccgccggcga tctcctgcgt gagggcctcg gccgcgtcgc
     2701 ggtcgcgcag gtagttgatg cacactgcgt agccgcgctg cgctgcgagc cgggcagtgg
     2761 cggcgccaat gccgcggccg ccgcccgtga tcatcagcac gccgctcatc gcccgcgcag
     2821 gaacagcttg tcgaggtcgc ccagcgtcag ctggtaccac gtcgggcggc cgtggttgca
     2881 gctgcccgag cgctcggttt gctccatctc gcgcagcaag gcattcatct ccggcaccga
     2941 cagctgccgg ttggcgcgca cagcggcgtg gcaggccatg gtggcgagga gctcgttctg
     3001 gcgcgcggcg agcacctgcg cggcgccgaa ttcgcgcagc tcggcgagca gcgagcgcac
     3061 gagcaccggc acgtcgccgc cggcgagcag cgccggcgcc gcgcgcaccg cgagctcgtt
     3121 cggcccggcc accgagagct ccagacccag cgtctccagc gcgccgcggt tctcttccgc
     3181 cgtggcgatg tcgagcgctt cggcgcggaa caccgcggga accagcaggc tctggcgctc
     3241 gatgccgccc gcatccagcc cgagcttcat cttctccatc aggatgcgct cgtgcgccgc
     3301 gtgcatgtcc accagcacga ggccggcctc gttctgcgcc aggatgtaca cgccatgcag
     3361 ttgcgcgagc gcatagccga gcggtggcgc cttctgcgcg gcgggcaggg gcgaagccgc
     3421 cggcgccggc cggtagcccg gcgtcggctc gcgcatcgcg agcgccgcct ggatcgcggg
     3481 gcccggcgcc gagacgctgg cgtagctcac gggcgcatcg gaggccgacg gcgcgagcgc
     3541 gcgctgcagc gcgtgcagaa cgaactggtg cacgccacgc gagtcgcgaa agcgcacctc
     3601 ggtctttgcc ggatgcacgt tgacgtcgac accgcgcgga tcgagctcga gcgcgagcac
     3661 gaacgccggc tggcgctcgc cgtgcagcgt gtcgcggtag gcttcgcgca ccgcgtgcac
     3721 gagcaggcgg tcgcgcacgt agcgcccttt gacgaagaaa tactgcgcgt cggcgcggct
     3781 gcgcgccgac tgcggc
//
LOCUS       DBNHKE010000188        11605 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1461263, whole genome shotgun sequence.
ACCESSION   DBNHKE010000188 DBNHKE010000000
VERSION     DBNHKE010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1461263"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /gene="pdxH"
                     /locus_tag="AC16O9_12825"
     CDS             <1..410
                     /gene="pdxH"
                     /locus_tag="AC16O9_12825"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR6517538.1"
                     /translation="TNYRSRKGRELAEQPLACLSFLWAELERQVRIEGRVAQVSSQES
                     DDYFASRPLGARLSAWAAAQSETVADRPTLERALEAMRAKYGDQPPRPPHWGGYRLLP
                     EAIEFWQGRADRLHDRLLYRRGAGGWSIERLAP"
     gene            391..1377
                     /locus_tag="AC16O9_12830"
     CDS             391..1377
                     /locus_tag="AC16O9_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6517539.1"
                     /translation="MSDSLPDPVLAFLRGAGLAAADETPAASVLTGGVSSDIWKVELR
                     RGPACVKRALPRLRVAQLWEAPVERNRFEWRWMQVAARLAPGSAPRVLAHDAAGLFAM
                     EYLGGERFTLWKHELRAGRADPSFARQVGTRLARIHAASAGDAGLAKEFATDEGFHAI
                     RLEPYLLATARRHPDLAARLRHLAERTAATRTALVHGDVSPKNILAGPQGPVFLDAEC
                     AWYGDPAFDLAFCLNHLLLKCLWVPQAAERFMACFDALAAGYLSVHGPAGLEARAASL
                     LPGLLLARVDGKSPVEYVTEEAQKARVRSVARTLLVAPPTTLAAVRSAWHGS"
     gene            1362..2591
                     /gene="eno"
                     /locus_tag="AC16O9_12835"
     CDS             1362..2591
                     /gene="eno"
                     /locus_tag="AC16O9_12835"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6517540.1"
                     /translation="MARIVDVRARRVWDSRGRATVEAEVVLKGARGRAIAPAGASTGA
                     GEAKALPADAAVRNVNGPMREALLGVDARFQEAVDEKLVALDGTPDRSRFGANAIVAV
                     SLACLQASAAAAKRPLWRHLAGERRVAMPVPQVQIYGGGAHAHGRVDIQDYLVMCPGA
                     GSFAESLEWIAEVYRAAGARLAKKGARHGVADEGGWWPSFKSNEEGLAELVGAISDAG
                     FEPGADVAIALDVAATQFHRGGRYHLALENRSLSAEQLHAMLMRWLDAYPIVSIEDPF
                     AEDDHDAMRAFTRAAGARVQIVGDDLFVTRAAKLDPELANAVLLKPNQVGTVTETLAC
                     WTGAKDKGMRGIVSARSGETEDVSIVHLAVGWGVPQLKVGSFARSERMAKWNEGLRIE
                     EDLGNKALLYPARKLFK"
     gene            complement(2775..3431)
                     /locus_tag="AC16O9_12840"
     CDS             complement(2775..3431)
                     /locus_tag="AC16O9_12840"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517541.1"
                     /translation="MLRLSGVSKRFGGRTVLKAVSMEVAPGEQVAVLGESGIGKSTLL
                     NVIAGLEPVDSGSIAYDGFEIAALSDDAATQLRREHFGFVFQAFHLLPYLTAEQNVGL
                     PLVLRGLDPAEIRRRARTLIGAVGLGGREAAMPRELSGGELQRIAIARALVGEPKLVL
                     ADEPTGNLDPDNARQVLEVLRGQVKQHGAAGILVTHSEAAAATCDRRYRLDADGLRAL
                     "
     gene            3570..5669
                     /locus_tag="AC16O9_12845"
     CDS             3570..5669
                     /locus_tag="AC16O9_12845"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6517542.1"
                     /translation="MTAWLAALLACAGLIAAYAGSAITTTVERLDDSESVRADTLLRE
                     RALGGTPQLPNETLIVRSAALSVDDAAYRAHVEAIAAQLLGLGPEVVSWGASYYLSGD
                     EALVSADRRSTLIPLVLRRPREDIARVREAARAASTDGRFELYSVGRASIGADYAALA
                     RDDLKAEVRIGLPAAIAVLLVVFGSVIAALLPVLVALAAIGVALAATALIGQLSPVYF
                     LATNMIVMMGLALGIDYSLFILSRYREERRAGRDKLDAIDAAGRSAARAVLYSGATVV
                     LALAGLLLVPTNVFRSLGTGAILVTATAVLASFTLLPAMIGLLGDRLEALRVPLPRMP
                     GAARRRGFWDGVTATVMRWPLASFAVAAALLLAAAAPTLRMNAGFAGVETLPERLESR
                     QGFRILQRDFHYGVLSVTRIVVDGDLGAPATRAALGALRAALAADAKFLAAGSTLRAD
                     AAGRTAVLAVPMAGDAEDGAVQEAVRRLRQDTIPRLFADAPARVLVAGNAAGYIDFFA
                     LTADYTPRVFAFVLGTSFLLLLAVFRSIAVPLVAIALNLLSVGAAYGLMVAVFQLGIG
                     ADLLGFRPAEAIEAWIPLFLFAVLFGLSMDYHVFLLSRIRERFEQTRRNDDAVAFGLQ
                     RSAGIITGAALIMVAIFAGFASGELVMFQQAGFGLAVAVLLDATLVRAVLVPSAMSLL
                     GERNWALPRWLEWLPRL"
     gene            5733..6914
                     /locus_tag="AC16O9_12850"
     CDS             5733..6914
                     /locus_tag="AC16O9_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6517543.1"
                     /translation="MHTPIRMLAAGLAAASLAFAPLASAQQKIKIGFITTLSGPAGVI
                     GKHMKDSVELALDHLGNKMGGVPVEVVFGDDQFKPDVGVQVAEEMLKKHQVDIMSGII
                     WSHVMMAVVPVVTDSGKVMVGTNAGASPLAGKACHPQFFSTSWNNDQSPEAMGKFMQE
                     QGINDVYILAPNYQAGKDMVAGFKRYYKGRIAEEIYTKPGQQDYQAEITQLRAKSPKA
                     TFVFYPGGMGIQFVKQYVQAGLRDRIPLYSVFTVDETTLPALKEAALGQYEARFWSPD
                     MNVPASKRFVADFEKKYGYTPSFYGAQSYDGMMLIDSAVRLNKGSLADTKAVVAAMRK
                     AEYDSVRGPFKWNVNQMPIQDFFLLRAEKGKDGAVEMKIQKKVFDDHKDAYYQDCKMA
                     W"
     gene            6980..7903
                     /locus_tag="AC16O9_12855"
     CDS             6980..7903
                     /locus_tag="AC16O9_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517544.1"
                     /translation="MNLILVFEQLLNGVQFGVMLFLMAAGLTLVFGIMNLVNLAHGSL
                     YMVGAYLMVAATQWTGNYLAGVALGLLGTLVVGMLVEVIALRTLYERDHLDQVLATFG
                     LILFFNELVAIIWGRTALYTSLPSWLQGHIEIIPGSIYPLYRAVVIGVGLLVGAILWY
                     VVARTRLGMLIRAGASNRTMVAALGVNIRLLYTVVFGFGAALAGLAGLMAGPIYAVQP
                     GMGELILIQVFVVIVIGGIGSIRGALVGALVVGVVDTLGRAFLKPLLGHFISPPAAEA
                     AGPALASMLIYLLMAIVLAMRPEGLFPAKSG"
     gene            7900..8838
                     /locus_tag="AC16O9_12860"
     CDS             7900..8838
                     /locus_tag="AC16O9_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517545.1"
                     /translation="MSARALVLAALVALLAAVPLIATLGGQPYYIDLIRRVMVFAIAA
                     ASLNLILGYGGLVSFGHAAYLGVGGYAVGIFAFYGIHNGWLQWAVAIAASALVALVIG
                     AISVRTRGIYFIMITLAFTQMLYYLGVSVEEYGGDDGMRLKVKSQFSGLVDLDDPVAF
                     YYVVLALLLGVIALVHRLVNSRFGMVLRAAKSNEARSRALGFSPYPYQLVAFVISGAI
                     CGLAGALLANHTAYIAPAFMEWTRSGEIMFMVILGGMGTTPGPALGAFALLLIEDLLA
                     GWTQHWQVILGPLLVLSVLFFRRGLAGLLPRGGRDG"
     gene            8831..9580
                     /locus_tag="AC16O9_12865"
     CDS             8831..9580
                     /locus_tag="AC16O9_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517546.1"
                     /translation="MAEAILEVRGLRKAFGALAATDGVDLDLREGETHAIIGPNGAGK
                     TTLIGQLAGDLRPDAGSIRFAGADVTRLTAPQRAQRGFARSFQITSIYRDFTALDNVA
                     LAVQAHAGHSFHFWRPARAERALREPARAVLEEVGLGARADVLAANLAHGEQRQLEVA
                     MALATGPRLLLLDEPVAGMGLEESQRMVQFLSSLKGKRSIILVEHDMDAVFVLADRIS
                     VLVYGRIIASGAPAEVRANPEVRRAYLGEEA"
     gene            9597..10298
                     /locus_tag="AC16O9_12870"
     CDS             9597..10298
                     /locus_tag="AC16O9_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517547.1"
                     /translation="MLKVEGLETAYGQQQVLFGVSLEVAAGEVVTLLGRNGMGKTTTV
                     LSLMGVVRPKGGRVTMQGRTISGLASYRVAKAGLGLVPEGRQVFPNLTVRENLVATAA
                     NRGGAGAAWTLERVFGLFPGLQERRRHYGNQLSGGEQQMLAIGRALMTNPKLLILDEA
                     TEGLAPLVRADIYLSIERLKAEGLSILVIDKDVRALTRIADRHYVLEKGRVVWTGSSA
                     ELGENRDVQHRYLGV"
     gene            10301..>11605
                     /locus_tag="AC16O9_12875"
     CDS             10301..>11605
                     /locus_tag="AC16O9_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR6517548.1"
                     /translation="MISREQNELMTRVGPGTPAGNLLRNYWQPVALTDELAGERPVKA
                     VRLLGEELVLFKAGGYRLMQRHCPHRGADLAYGRIEADGLRCAFHGWKFDATGRCVDT
                     PAEPAGSRMCEQIRPVSYPVVERSGMLFAYLGAGEPPAFAHFDCFTAPDTHTFAFKGY
                     WDCNWLQALEVGIDPAHASYLHRYFEDEDTATSYGKQFRGKPSDADLPISKVLREYDR
                     PEITVARTDYGIRLVTLRRIDEQKTHGRATHLVFPQAFVIPMNAEMTISQWHVPVDDT
                     GCYWYAIFTSFTSPVDKKTMREQRLKTYPAPDYKPVFNRDNGWGFNAEEQRKQTYTGM
                     GFDINVHDQWACESPGRLADRTKENLGTTDKGIVLYRRLLVDAIQKAAKGERTLMMLD
                     GAQASALTGPPAVDGVGPTGRWDEYCREADAARRNRAPWSAKA"
BASE COUNT         1661 a         4124 c         4007 g         1813 t
ORIGIN      
        1 atacgaacta ccgcagccgc aagggccggg agctggcgga gcagccgctc gcctgcctgt
       61 cgttcctgtg ggcagagctc gagcggcagg tgcgcatcga gggccgcgtc gcgcaggtgt
      121 cgtcgcagga gtccgacgac tactttgcca gccggccgct cggcgcccgc ctgtcggcat
      181 gggccgcggc acaaagcgag accgtggccg accgccccac gctggagcgc gcactggagg
      241 cgatgcgcgc gaagtacggc gatcagccgc cgcgccctcc gcattggggc ggctaccgcc
      301 tgctgcccga ggcgatcgag ttctggcagg gacgcgccga ccggctgcac gaccgcttgc
      361 tctaccggcg cggcgccggg ggctggtcga ttgagcgact cgctccctga cccggtgctc
      421 gcgttcctgc gcggtgccgg gctcgccgct gcggacgaaa cgcccgcggc gagcgtgctg
      481 accggcggcg tgtcttcgga catctggaag gtcgagctgc gtcgcgggcc ggcgtgcgtg
      541 aagcgagcgc tgccgcgcct gcgcgtggcg cagctctggg aggcgccggt cgagcgcaat
      601 cgctttgaat ggcgctggat gcaggtggcg gcgcgcctag cgccggggag cgcgccgcgt
      661 gtgctggcgc acgacgcggc gggactgttt gccatggaat acctgggcgg cgagcgcttt
      721 acgctctgga agcatgagct gcgcgcgggc cgcgccgacc ctagcttcgc gcgccaggtg
      781 gggacgcggc tcgcgcgcat ccatgccgcc agcgcgggcg atgcgggcct cgcgaaagag
      841 ttcgcgaccg acgagggctt tcacgccatc cgcctggagc cctacctcct cgccaccgcg
      901 cgccgccacc ccgacctcgc tgctcggctg cggcatctcg ccgagcgcac agccgcgacg
      961 cgcaccgcgc tggtgcatgg cgacgtcagc ccgaagaaca tcctcgccgg cccgcaaggc
     1021 ccggtctttc tcgatgccga atgcgcgtgg tacggcgatc cggcgttcga cctcgctttc
     1081 tgcctgaacc acctcctgct gaagtgcctg tgggtgccgc aagccgccga gcggttcatg
     1141 gcgtgcttcg acgcgcttgc ggcgggctat ctctcggtcc acggccccgc cgggctggaa
     1201 gcgcgcgcag cgagcctgct gcccgggctg cttcttgcgc gcgtcgacgg caagtcgccg
     1261 gtggaatacg tgaccgagga ggcgcagaag gcgcgggtgc gctcggtcgc acgcacgctg
     1321 ctcgtcgccc cgcccacgac actcgccgca gtgcgcagcg catggcacgg atcgtagacg
     1381 tccgcgcgcg gcgcgtgtgg gactcgcgcg gccgcgccac ggtcgaagcc gaagtggtgc
     1441 tcaagggcgc gcgcggccgc gccatcgccc ccgcgggcgc ctccaccggg gcgggcgaag
     1501 cgaaggcgct gccggccgat gccgccgtgc gcaacgtgaa cggcccgatg cgcgaggcgc
     1561 ttctcggggt ggatgcgcgc ttccaggagg cggtcgacga gaagctggtc gcgctcgacg
     1621 gcacgccgga cagatcgcgc ttcggcgcca acgccatcgt cgcggtgtcg ctcgcctgcc
     1681 tgcaggcgtc ggcggcggcg gcgaagcggc cgctgtggcg ccacctcgcc ggcgagcgcc
     1741 gcgtcgccat gccggtgccg caggtgcaga tctacggcgg gggcgcgcac gcgcacggcc
     1801 gcgtcgacat ccaggactac ctggtcatgt gtcccggcgc cggcagcttt gccgagagcc
     1861 tggagtggat tgccgaggtg taccgcgcgg caggggcgcg cctggcgaag aaaggcgcgc
     1921 ggcacggggt cgccgacgaa ggcggctggt ggccttcctt caagtccaac gaggaaggcc
     1981 tcgccgagct ggtcggcgcg atcagcgacg ccggcttcga gcccggtgcg gacgtcgcca
     2041 tcgcgctcga cgtggcggcg acgcagttcc accgcggcgg gcgctaccac ctcgcgctcg
     2101 agaaccgttc gctcagcgcc gagcagctgc acgccatgct catgcgctgg ctcgatgcct
     2161 atccgatcgt ctcgatcgag gacccgttcg ccgaggacga ccacgatgcg atgcgcgcct
     2221 tcacgcgcgc ggcgggcgca cgcgtgcaga tcgtcggcga cgacctgttc gtgacgcgcg
     2281 cggcgaagct cgatcccgag ctcgccaacg ccgtgctgct caagcccaac caggtcggca
     2341 ccgtcaccga aacgctcgcc tgctggaccg gcgcaaagga caagggcatg cgcggcatcg
     2401 tgtcggcgcg ctcgggcgag accgaggacg tctcgatcgt gcacctggcg gtgggctggg
     2461 gcgtacccca gctcaaggtg ggcagcttcg cgcgctccga gcgcatggcg aagtggaacg
     2521 agggcctgcg catcgaggag gacctcggca acaaggccct cctctacccg gcgcgcaagc
     2581 tctttaaata gggacagacc tcattttccc ggcggcgacg caagcgctac cgcgccgccg
     2641 ttgcctttca aaacgatctc ggcttttctg gctggaagat tcacccagcc agaaaagccg
     2701 agaccataga gaaccgaacg caggcttcag cgccttcgag acgccggcca agagaaaatg
     2761 aggtctgtcc ccatttaaag cgcccgcagg ccgtcggcgt ccaggcgata gcgccggtcg
     2821 caggtcgctg cggcggcttc ggagtgcgtg acgaggatgc cggcggcgcc gtgctgcttg
     2881 acctgcccgc gcagcacttc cagcacctgg cgcgcgttgt ccgggtcgag gttgccggtg
     2941 ggctcgtcgg ccagcaccag ctttggctcg cccaccagcg cgcgcgcgat ggcgatgcgc
     3001 tgcagctcgc cgcccgagag ctcgcgcggc atcgcggctt cgcgcccgcc caggcccacc
     3061 gcgccgatca gcgtgcgcgc gcgacgccgg atctccgctg ggtcgaggcc gcgcagcacc
     3121 agcggcaggc cgacgttctg ctccgcggtg aggtagggca gcaggtggaa cgcctggaac
     3181 acgaagccga agtgctcgcg ccgcagctgc gtggccgcgt cgtcggagag cgccgcgatc
     3241 tcgaacccgt cgtacgcgat cgagcccgag tcgaccggct cgaggccggc gatgacgttg
     3301 agcagcgtgg acttgccgat gcccgactcg ccgagcaccg cgacctgctc gccgggggcg
     3361 acttccatgg acacggcctt cagcaccgtg cgcccgccga accgtttgct gacgcccgag
     3421 agcctgagca tggcgcaagt ctagccgctc gccggactgt agacacgact cgacggactc
     3481 tgccagactc gaccggagag acgcatcgat aaatccgcgt gccgcccagc tcccttgccc
     3541 tcttctgcgc gcgccggccg ggagggatct tgacggcctg gctcgcggcg ctgctggcgt
     3601 gcgccgggct gatcgccgcc tacgccggca gcgccatcac caccacggtg gagcgcctgg
     3661 acgattcgga gtccgtgcgc gcggacacgc tgctgcgcga gcgcgcactc ggcggcaccc
     3721 cgcagctgcc caacgagacg ctgatcgtcc gttccgcggc tctgtccgtc gacgacgcgg
     3781 cgtaccgcgc gcacgtggag gcgatagccg cacagctcct cggtctcggt cccgaggtcg
     3841 tgtcctgggg cgccagctac tacctgtccg gcgacgaagc gcttgtatcg gcggaccgcc
     3901 gctccactct catcccgctc gtgctgcgtc ggccgcgcga ggacatcgcg cgcgtgcgcg
     3961 aggcggcacg cgccgcaagc accgacggcc gctttgaact gtattcggtg ggccgcgcca
     4021 gcatcggcgc ggactacgcg gcgctggcgc gcgacgacct caaggccgaa gtccggatcg
     4081 gcctgccggc cgcgatcgcc gtgctgctcg tcgtcttcgg ctccgtgatc gccgcgctcc
     4141 tgcccgtgct ggtcgcgctc gccgccatcg gcgtcgcgct ggccgcgacc gcgctcatcg
     4201 gccagctgtc gccggtgtac ttcctcgcca ccaacatgat cgtcatgatg gggctggcgc
     4261 tcggtatcga ctactccctg ttcatcctct cgcgctatcg cgaggagcgc cgcgccggcc
     4321 gcgacaagct cgatgccatc gacgcggccg ggcgtagcgc cgcgcgcgcc gtgctctata
     4381 gcggcgccac cgtcgtgctc gcgctcgccg gcctgctgct cgtaccgacc aacgtgttcc
     4441 ggagcctggg caccggcgca atcctggtga cggcgacggc ggtgctggct tccttcaccc
     4501 tgctgccggc gatgatcggg ctgctcggcg accggctcga ggcgctgcgg gtgccgctgc
     4561 cgcggatgcc gggcgccgcc cgaaggcgcg gcttctggga cggcgtgacg gcgacggtca
     4621 tgcgctggcc gctggcgagt ttcgccgtcg ccgccgcgct gctcctcgcc gccgccgcgc
     4681 cgacgctgcg catgaacgcc ggcttcgccg gcgtcgagac cctccccgag cgcctcgaat
     4741 cgcgccaggg cttccgcatc ctgcagcgcg acttccacta cggcgtactc tccgtgacgc
     4801 gcatcgtggt cgacggcgat ctcggcgcgc ccgccacgcg ggcggcgctc ggcgcgctgc
     4861 gcgcggcgct ggcggcggac gcgaagttcc tcgcggccgg atcgaccctg cgcgcggacg
     4921 cggcgggccg caccgcggta ctggcggtgc cgatggccgg cgacgccgag gacggcgcgg
     4981 tgcaggaggc ggtgcgccgg ctgcgccagg acaccattcc gcgcctcttc gccgacgcgc
     5041 cggcacgcgt gctggtcgcc ggtaacgccg ccggctacat cgacttcttt gccctcaccg
     5101 ccgactacac gccgcgggtg ttcgccttcg tcctcggcac cagcttcctc ctgctgctcg
     5161 ccgtgttccg ctcgatcgcc gtgccgctgg tggcgatcgc cctcaacctg ctttcggtcg
     5221 gcgccgccta tggcctgatg gtggcggtgt tccagctcgg catcggcgcc gacctgctcg
     5281 gcttccgacc ggccgaggcc atcgaggcgt ggattccgct ctttttgttc gctgtgctgt
     5341 tcggcctgtc gatggactac cacgtgttcc tgctctcccg catccgcgag cgcttcgagc
     5401 aaacgcgccg caacgacgac gcggtagcct tcgggctgca gcgctcggcg gggatcatca
     5461 ccggcgcggc gctgatcatg gtggcgatct tcgccggctt cgccagcggc gagctggtca
     5521 tgttccagca ggccggtttc ggcctggcgg tggcggtgct ccttgacgcc accctggtcc
     5581 gggcggtgct cgttccctcg gccatgagcc tgctgggcga gcgcaactgg gccctgccgc
     5641 gctggctcga gtggctgccc cggctctagc ctagacatcg ctcgccgcgc cttgctagac
     5701 tggacgcgca cataaatcgg aggagattcc ccgtgcatac ccctatccgg atgttggccg
     5761 caggtctggc cgccgcttcg ctcgcgtttg cgccccttgc gtccgcgcag cagaagatca
     5821 agatcggctt catcaccacg ctgtcaggtc ccgccggcgt catcggcaag cacatgaagg
     5881 actcggtcga gctggcgctc gatcacctcg gcaacaagat gggcggcgtg cccgtcgagg
     5941 tggtcttcgg cgacgaccag ttcaagcccg acgtcggcgt gcaggtcgcc gaggagatgc
     6001 tgaagaagca ccaggtcgac atcatgtccg gcatcatctg gtcgcacgtg atgatggccg
     6061 tcgtgccggt ggtgaccgac tcgggcaagg tgatggtcgg caccaacgcg ggcgcgagcc
     6121 cgctcgccgg caaggcctgc cacccgcagt tcttctccac ctcctggaac aacgaccagt
     6181 cgcccgaggc gatgggcaag ttcatgcagg agcagggcat caacgacgtg tacatcctcg
     6241 cgcccaacta ccaggccggc aaggacatgg tggccggctt caagcgctac tacaaggggc
     6301 gcatcgccga ggagatctac accaagccgg gccagcaaga ttaccaggcc gagatcacgc
     6361 agctgcgcgc caagagcccg aaagcgactt tcgtgttcta cccgggtggc atggggatcc
     6421 agttcgtgaa gcagtacgtg caggcagggc tgcgcgaccg gatccccctg tactcggtgt
     6481 tcacggtgga cgagaccacg ctgccggcgc tcaaggaggc tgcgctcggc cagtatgagg
     6541 cgcgcttctg gagcccggac atgaacgtgc cggcgagcaa gcgcttcgtc gccgacttcg
     6601 agaagaagta cggctacacg ccctcgttct acggcgcgca gagctacgac ggcatgatgc
     6661 tgatcgactc cgcggtgcgc ctgaacaagg gcagcctcgc cgacaccaag gcggtggtcg
     6721 cggcgatgcg caaggccgag tacgactcgg tccgcgggcc gttcaagtgg aacgtcaatc
     6781 agatgccgat ccaggacttc ttcctgctgc gcgccgagaa gggcaaggac ggcgccgtcg
     6841 agatgaagat ccagaagaag gtgtttgacg accacaagga cgcctactac caggactgca
     6901 agatggcgtg gtgagcccac gggctccaca agcctgaagg gcgcggcgcc accgcgccct
     6961 tttttgtgct caatccgcga tgaacctgat cctggtcttc gagcagctgc tgaacggcgt
     7021 gcagttcggc gtcatgctgt tcctgatggc cgcggggctg acgctggtgt ttggcatcat
     7081 gaacctcgtc aacctcgcgc acggctcgct ctacatggtc ggcgcctacc tgatggtggc
     7141 ggccacgcaa tggaccggca actacctcgc cggcgtcgcg ctcgggctcc tcggcacgct
     7201 ggtcgtcggc atgctggtgg aagtgatcgc gctcaggacg ctctacgagc gcgaccacct
     7261 cgaccaggtg ctggcgacct tcgggctgat cctgttcttc aacgagctgg tcgccatcat
     7321 ctggggccgc accgcgctgt acacctcgct gccctcgtgg ctgcagggcc acatcgagat
     7381 catccccggc tcgatctatc cgctgtaccg cgcggtggtg atcggcgtcg gccttcttgt
     7441 cggcgccatc ctctggtacg tcgtggcgcg cacccgcctc ggcatgctga tccgcgccgg
     7501 ggcttccaac cgcaccatgg tggccgcgct cggcgtcaac atccggctcc tttacaccgt
     7561 ggtattcggt ttcggcgccg cgctcgccgg gctggccggc ctgatggccg ggccgatcta
     7621 cgccgtgcag cccggcatgg gcgagctgat cctgatccag gtgttcgtgg tcatcgtcat
     7681 cggcggcatc ggctcgatcc gcggcgcgct ggtcggcgcg ctggtggtcg gcgtggtcga
     7741 cacgctcggg cgcgccttcc tcaagcccct gctcgggcac ttcatctcgc cgcccgccgc
     7801 cgaggccgcg ggcccggcgc tcgcctcgat gctcatctac ctgctgatgg cgatcgtcct
     7861 cgcaatgcgt cccgagggcc tgttcccggc gaagagcgga tgagcgcgcg cgccctcgtc
     7921 ctcgccgccc tcgtggcgct gctcgccgcg gtgccgctca tcgcaacgct cggcggccag
     7981 ccctactaca tcgacctcat ccgccgcgtc atggtgttcg cgatcgccgc ggcgagcctc
     8041 aacctgatcc tcggctacgg cggcctggtg tccttcggcc atgccgcgta cctcggggtc
     8101 ggcggctacg cggtcggcat cttcgccttc tacggcatcc acaacggctg gctgcagtgg
     8161 gcggtggcga tcgcggcgtc cgcgctggtg gcgctggtga tcggcgccat ctcggtgcgc
     8221 acgcggggca tctacttcat catgatcacc ctcgccttca cgcagatgct gtattacctc
     8281 ggcgtgtcgg tcgaggagta cggcggcgac gacggcatgc gcctcaaggt gaagagccag
     8341 ttctccggcc tggtcgacct cgacgacccg gtcgccttct actacgtggt gctcgcgctg
     8401 ctgctcggcg tcatcgctct ggtgcaccgg ctcgtgaact cgcgcttcgg catggtgctg
     8461 cgcgcggcca agtcgaacga ggcgcgctcg cgcgccctcg gcttctcgcc ctacccgtac
     8521 cagctcgtcg ccttcgtcat ctctggcgcg atctgcgggc tggcgggggc gctgctcgcc
     8581 aaccacaccg cctacatcgc gccggcgttc atggaatgga cgcgctcggg cgagatcatg
     8641 ttcatggtga tcctcggcgg catgggcacc acgccggggc cggcgctcgg cgcctttgcg
     8701 ctgctactca tcgaggacct gctcgccggc tggacgcagc actggcaggt gatcctcggg
     8761 ccgctgctgg tgctctcggt gctgttcttc cggcgcgggc tcgccgggct gctgccgcga
     8821 ggcggccgcg atggctgagg cgatcctcga ggtgcgcggg ctgcgcaagg cgttcggcgc
     8881 gctcgcggcc accgacggcg tcgacctcga cctgcgcgag ggcgagacgc acgccatcat
     8941 cggcccgaac ggcgcgggca agaccacgct catcggccag ctcgcgggcg acctgcggcc
     9001 cgacgccggc agcatccgct tcgcgggcgc cgacgtgacg cggctcaccg cgccgcaacg
     9061 cgcgcagcgc ggctttgcgc gctcgttcca gatcaccagc atctaccgcg acttcaccgc
     9121 gctcgacaac gtcgcgctcg cggtgcaggc gcacgccggg cacagcttcc acttctggcg
     9181 ccccgcgcgc gcggagcgcg cgttgcgcga gccggcacgc gcggtgctcg aagaggtggg
     9241 gctcggcgcg cgcgccgacg tgctcgccgc caacctcgcg cacggcgagc agcgccagct
     9301 cgaagtggcg atggcgctcg ccacgggccc gcggctgctg ctgctcgacg agccggtggc
     9361 cgggatgggg ctggaggagt cgcagcgcat ggtgcagttc ctctccagcc tcaagggcaa
     9421 gcgcagcatc atcctcgtcg agcacgacat ggacgccgtg ttcgtgctcg ccgaccgcat
     9481 ttcggtgctg gtgtacggcc gcatcatcgc gagcggcgcg ccggccgaag tgcgcgccaa
     9541 ccccgaagtg cgccgcgcct acctcgggga agaagcctga tgcgagtgaa gtccacatgc
     9601 tgaaggtcga gggcctcgag accgcctacg gccagcagca ggtgctgttc ggcgtgtcgc
     9661 tggaggtcgc cgccggcgag gtggtcacgc tgctcgggcg caacggcatg ggcaagacca
     9721 ccacggtgct ttcgctcatg ggcgtggtgc gccccaaggg cgggcgcgtc accatgcagg
     9781 gcaggacgat ctccggcctg gcctcctacc gcgtggccaa ggccggcctg ggcctggtgc
     9841 ccgaggggcg gcaggtgttt cccaatctca cggtgcgcga aaacctcgtg gccaccgccg
     9901 cgaatcgcgg cggcgcggga gcggcgtgga cgctggaacg cgtcttcggc ctgtttcccg
     9961 gcctgcagga acgccgccgc cactacggca accagctctc cggcggcgag cagcagatgc
    10021 tggccatcgg gcgcgcgctg atgaccaacc cgaagctgct cattctcgac gaagccaccg
    10081 agggcctggc gccgctggtg cgcgccgaca tctacctttc gatcgagcgg ctgaaagccg
    10141 aaggcctgtc gatcctcgtc atcgacaagg acgtgcgcgc gctgacgcgc atcgccgacc
    10201 ggcactacgt gctggagaag ggccgcgtgg tctggaccgg cagctcggcc gagctgggcg
    10261 agaatcgcga cgtgcagcac cgctacctgg gggtctagcg atgatcagcc gggaacagaa
    10321 cgagctcatg acccgcgtcg gcccgggcac gcccgccgga aacctgctgc gcaactactg
    10381 gcagccggtg gcgctgaccg acgagctcgc cggcgagcgg ccggtgaagg ccgtgcgcct
    10441 gctcggcgag gagctggtgc tcttcaaggc cggcggctat cggctgatgc agcgccattg
    10501 cccgcaccgc ggcgccgacc tcgcctacgg ccgaatcgaa gccgacggcc tgcgctgcgc
    10561 cttccacggc tggaagttcg atgcgaccgg gcgctgcgtg gacacgcccg ccgagcccgc
    10621 gggcagccgc atgtgcgagc agatccgtcc cgtgtcgtat ccagtagtgg agagaagcgg
    10681 catgctcttc gcctacctcg gcgcaggcga gccgcccgcg tttgcgcact tcgactgctt
    10741 caccgcgccc gacacgcaca ccttcgcctt caagggctac tgggactgca actggctgca
    10801 ggcgctcgaa gtcggcatcg accccgcgca cgcctcgtac ctgcaccgct acttcgagga
    10861 cgaggacacc gcgacgagct acggcaagca gttccgcggc aagccgtccg acgccgatct
    10921 gccgatctcc aaggtgctgc gggagtacga ccggcccgaa atcacggtcg cgcgcaccga
    10981 ttacggcatt cgcctcgtga cgctgcgcag gatcgacgag cagaagaccc acggccgcgc
    11041 cacgcacctc gtcttcccgc aggccttcgt catcccgatg aacgccgaga tgacgatctc
    11101 gcagtggcac gtgccggtgg acgacaccgg ctgctactgg tacgcgatct tcaccagctt
    11161 cacctcgccc gtggacaaaa agaccatgcg cgagcagcgg ctgaagacct atccggcgcc
    11221 cgactacaag ccggtgttca atcgcgacaa cggttggggc ttcaatgccg aagagcagcg
    11281 caagcagacc tacaccggca tgggcttcga catcaacgtc cacgaccagt gggcctgcga
    11341 gtcgcccggg cgcctcgccg atcgcacgaa ggagaacctc ggcacgacgg acaagggcat
    11401 cgtgctctac cggcgactcc tggtcgacgc gatccagaag gcggcgaagg gcgagcgcac
    11461 gctgatgatg ctcgacggcg cgcaggcttc ggcgctcacc ggcccgcccg cagtggacgg
    11521 tgtcgggccc accgggcgct gggacgagta ctgccgtgaa gccgacgctg ctcggcgcaa
    11581 ccgcgcgccg tggtcggcga aggcg
//
LOCUS       DBNHKE010000189         3140 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1037447, whole genome shotgun sequence.
ACCESSION   DBNHKE010000189 DBNHKE010000000
VERSION     DBNHKE010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1037447"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16O9_12880"
     CDS             complement(<1..189)
                     /locus_tag="AC16O9_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR6517549.1"
                     /translation="MSGFAALIPARFASTRFPGKPLADLAGKPMVVRVCERARESGAA
                     PVCVATDDKRIADAVRAPG"
     gene            complement(186..368)
                     /locus_tag="AC16O9_12885"
     CDS             complement(186..368)
                     /locus_tag="AC16O9_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR6517550.1"
                     /translation="MDPRLLEILVCPLCKGPLVYRKAEGELVCKPDRLAYPVKDGIPV
                     MLEDDARKLDPTEEIA"
     gene            complement(349..1293)
                     /gene="lpxK"
                     /locus_tag="AC16O9_12890"
     CDS             complement(349..1293)
                     /gene="lpxK"
                     /locus_tag="AC16O9_12890"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR6517551.1"
                     /translation="MLWPLSLIFRAAVFARRSLYWLRLLKSSRAGVPVIAIGNLTVGG
                     AGKTPLAIHVAELLKAKRWSPAIVSRGYGGTVTAPRGVTLAADPAEVGDEPVLMARRS
                     GCPVWVGQQRAAVIAALRQSHPECDVVILDDGLQHYALRRDLEIAVVDARGFGNGFML
                     PAGPLREPKTRLWSVDAVIAHGTDKVKGYAMRLEGDEVHRATDARERRPVKSFAGERV
                     HAVAGIGDPTRFFQHLARLGVQAVPHPFPDHHPFRAGDLEFGDDAPVLMTEKDAVKCK
                     RFAKPRHWVLPVRAVPDPAFDSWLQRRLGELGHRPKAA"
     gene            complement(1344..1760)
                     /locus_tag="AC16O9_12895"
     CDS             complement(1344..1760)
                     /locus_tag="AC16O9_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6517552.1"
                     /translation="MNFQRDKEREPLEINLVPMIDVMLVILIFLMITTTYSKYTELQI
                     NLPAADADKQLERASEIAVLVNAQGQYIINRTPVAFVSIEQLAGELKRASGAAKEPVV
                     VVSADANATHQSVVRVMEAARAAGLQQITFTTQASR"
     gene            complement(1769..2386)
                     /locus_tag="AC16O9_12900"
     CDS             complement(1769..2386)
                     /locus_tag="AC16O9_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6517553.1"
                     /translation="MFAIIQAAGWPIWPLLLASVIAVALIIERSVSLRSSKIIPPTLL
                     EQVLAVYQRQGVNEDVLDKLAKDSPLGEVLAAGLRNRRSSRYVMKEAVEEAGRTVAHE
                     LERFLTTLGTIATASPLLGLFGTVIGMIEIFGSQSPTGGTNPQQLAHGISIALYNTAF
                     GIGIAIPALIFYRHFRNKVDGFVVDMEQQAAKLIDIVHGERTEHA"
     gene            2307..2627
                     /locus_tag="AC16O9_12905"
     CDS             2307..2627
                     /locus_tag="AC16O9_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517554.1"
                     /translation="MISATAMTEASNKGQMGQPAAWIMANKMALRGLWFLLSAHFSKK
                     AERALSTFPVDKFVDFLWAAHGLPRARHAKSVAIKNSSILSGTETAAYEIESGSVRDR
                     RAPL"
     gene            2661..>3140
                     /gene="xseA"
                     /locus_tag="AC16O9_12910"
     CDS             2661..>3140
                     /gene="xseA"
                     /locus_tag="AC16O9_12910"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6517555.1"
                     /translation="MDEELLGASAPVLSVSALLRSVRDTLERRFPLAWIGGEISNFRP
                     ASSGHWYFTLKDEAAQVDCVMFRSRTAALDWEPAEGMRVEARALVTLYEPRGRFQLNV
                     EHMRRAGLGPLYERFLKLKAQLEKEGLFDPAAKREIPAFPTTIGVVTSRQAAALRDVL
                     "
BASE COUNT          498 a         1042 c         1070 g          530 t
ORIGIN      
        1 cccgggcgcg cgcacggcgt ccgcaatgcg cttgtcgtcg gtagccacgc acaccggcgc
       61 ggcgccgctt tcgcgcgcgc gctcgcatac gcgcaccacc atcggcttgc cggcgagatc
      121 ggcgagcggt ttgccgggaa agcgggtgga ggcgaagcgc gcggggatca gcgccgcgaa
      181 gccgctcacg cgatctcttc ggtcggatcg agcttgcgcg cgtcgtcctc gagcatcacc
      241 ggaatgccgt ccttcaccgg gtaggcgagg cgatcgggct tgcataccag ctcgccctcg
      301 gccttgcggt acacgagcgg ccccttgcac agcgggcaca ccaggatttc aagcagcctt
      361 gggtctatgg ccgagctcac cgagcctcct ttgcagccat gaatcgaacg ccgggtcggg
      421 caccgcgcgc accggcagaa cccagtgccg cggtttcgcg aagcgcttgc attttaccgc
      481 gtccttttcc gtcatcagca ccggcgcgtc gtcgccgaac tcgagatcgc ccgcgcgaaa
      541 cgggtggtgg tcgggaaacg ggtgcggcac cgcctgcacg ccgagccgcg cgaggtgctg
      601 gaagaagcgc gtcgggtcgc cgatgccggc caccgcgtgc acgcgctcgc cggcaaagga
      661 cttcaccggc cggcgctcgc gcgcgtcggt ggcgcggtgc acttcgtcgc cctcgagccg
      721 catggcgtag cctttgacct tgtcggtgcc gtgagcgatg acggcatcca cgctccacag
      781 gcgcgtcttc ggctcgcgca gcggccccgc cggcagcatg aagccgttgc cgaaaccgcg
      841 cgcgtccacc accgcgatct cgaggtcgcg ccgcagcgcg tagtgctgca ggccgtcgtc
      901 gaggatcacg acatcgcatt cgggatgcga ctgccggagc gccgcgatca ccgcggcgcg
      961 ctgttgcccg acccataccg ggcagccgct gcggcgcgcc atcagcaccg gctcgtcgcc
     1021 gacctcggcc gggtcggcgg cgagcgtcac gccgcgcggc gcggtgacgg tgccgccgta
     1081 accgcgcgag acgatcgccg gcgaccagcg cttcgccttg aggagctcgg cgacatggat
     1141 ggcgagcggc gtcttgccgg ctccgccgac ggtgaggttg ccgatggcga tcaccggcac
     1201 gcctgcgcgc gagctcttca gcaggcgcag ccagtacagc gatcgccgcg caaacacggc
     1261 cgcgcgaaat atgaggctca gcggccacag cacccagctg acggggttct gccggtacca
     1321 gagcggcgcg aggaggcgcg tggctagcgg ctcgcctggg tcgtgaaggt gatctgctgc
     1381 aggccggcag cgcgtgcggc ttccatcacg cgcaccaccg actggtgcgt ggcgttggcg
     1441 tcggcgctca ccaccaccac cggctccttg gcggcgccgc tcgcgcgctt cagctcgccc
     1501 gcgagctgct cgatgctgac gaaagcgacc ggggtgcggt tgatgatgta ctggccctgc
     1561 gcgttcacca gcaccgcgat ctcgctcgcg cgctcgagct gcttgtcggc gtcggcggcc
     1621 ggcagattga tctgcagctc ggtgtacttg gagtaggtgg tggtgatcat gaggaagatc
     1681 aggatcacca gcatcacgtc gatcatcggc accaggttga tctcgagggg ctctcgctct
     1741 ttgtcgcgct ggaagttcac gcgcgcctct aggcgtgctc ggtgcgctcg ccgtgcacga
     1801 tgtcgatcag cttcgccgcc tgctgctcca tgtcgacgac gaagccgtcg accttgttcc
     1861 ggaagtggcg gtagaagatg agcgcgggga tggcgatgcc gatgccgaac gcggtgttgt
     1921 atagagcgat ggagatgccg tgcgcgagct gctgcgggtt ggtgccgccg gtgggcgact
     1981 gcgagccgaa gatctcgatc attccgatca ccgtgccaaa caggcccagc agcggcgagg
     2041 ccgtggctat ggtgccaagc gtggtcagaa agcgctccag ctcgtgcgcc acagtgcgcc
     2101 cggcctcctc gaccgcttcc ttcatcacgt accgcgagct gcggcgattg cgcaggcccg
     2161 cggccagcac ttcgccgagc ggggagtcct tggcgagctt gtcgagcacg tcctcgttca
     2221 ccccttggcg ctggtacacg gcgagcacct gctccagaag cgtcggcggg atgatcttgc
     2281 tgctgcgcag cgagaccgag cgctcgatga tcagcgccac cgcaatgacg gaggccagca
     2341 acaagggcca gatgggccag cctgcagctt ggataatggc gaacaagatg gcgctccgtg
     2401 gtctctggtt cttgttgtcg gcgcacttta gcaaaaaggc ggagcgcgcg ttatccacat
     2461 ttcctgtgga taagtttgtg gattttcttt gggcagcgca cggcttgcct cgcgcacggc
     2521 acgccaagtc ggtggcgatt aaaaattcat caatcctttc cggcacggaa acagcggctt
     2581 acgaaataga atcgggctcc gtaagggatc gccgcgcgcc gctttgacgc gcggccgcgc
     2641 ttgtgcatta gtgtcgccgc gtggacgaag aattattagg cgcttcggcg ccagtgctga
     2701 gcgtgtcggc gctgctacgc agcgtgcgcg atacgctcga gcggcgcttc ccgctcgcct
     2761 ggatcggggg cgagatctcc aacttccgcc cggcctcctc cgggcactgg tatttcaccc
     2821 taaaagacga ggccgcccag gtggactgcg tgatgttccg tagccgcact gcagcattgg
     2881 actgggagcc ggccgaaggc atgcgcgtcg aggcccgggc cctggtcacg ctgtacgagc
     2941 cgcgcggacg cttccagctg aacgtcgagc acatgcgccg cgcgggcctg ggaccgctct
     3001 acgagcgctt cctgaagctc aaggcgcagc tggaaaagga aggtctattc gatccggcgg
     3061 ccaaacgcga aatccctgcg tttcccacga cgatcggtgt cgtcacatca cgacaggcgg
     3121 ccgcactgcg cgacgtgcta
//
LOCUS       DBNHKE010000190        33816 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1250404, whole genome shotgun sequence.
ACCESSION   DBNHKE010000190 DBNHKE010000000
VERSION     DBNHKE010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 33816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33816
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1250404"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2744
                     /locus_tag="AC16O9_12915"
     CDS             <1..2744
                     /locus_tag="AC16O9_12915"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR6517556.1"
                     /translation="RLQRVPGSGGAVRDVAHAPIVEAFAQRAKRGPARHAAQTAPDRK
                     QVSVIQLVAEYRFRGCLVADLDPLRRLPKPHIVELEPGYYDLGEADLDTPFNTGTYMG
                     AEQATLREIIQHLRDTYCGTLGVEYMYLSSRAEKRWIQERLEPIRSRPSFSAEEKKHI
                     LERLSAAEGLERYLHTRYVGQKRFSLEGGDTLIPVLDHLLQRAGAAGVQELVLGMAHR
                     GRLNVLVNILGKMPKTLFREFEGEHDASVLAGDVKYHQGFSSDINTPGGPMHLTLAFN
                     PSHLEIVNPVVEGSVRARQHRRKDRDGSQVLPVLIHGDAAVAGQGVVQETLNLSQTRG
                     YGTGGTVHIVINNQIGFTTSDARDVRSTLYCTDVMKMIEAPIFHVNGDDPEAAVMAIE
                     MALDFRTQFRKDVVVDLVCFRKLGHNEQDEPMVTQPLMYKIINKHPGTRKLYAERLLA
                     QGVIEPDEADKVAANYRQALEGGFHTNKTILSNYKPPFAVDWSKFRGTKWNEHDDTRI
                     PLAALQALAERLTAVPPNFKLHPRVEKIIADRRLMGQGKIPVDWGMAENLAYASLLKD
                     GYAVRISGQDSGRGTFFHRHAVLHDQNRERFDAGTYVPLQHIAPNQGDFVVIDSVLSE
                     EAVLGFEYGYSTSEPNELVVWEAQFGDFMNGAQVVIDQFIASGEVKWGRICGMVMMLP
                     HGYEGQGPEHSSGRIERFLQLCADYNLQVCMPATPVQLFFLLRRQMIRPYRKPLVIFT
                     PKSLLRHKESVSPISEFAESKFKPVNAEWNEDLKPDKVTRVIFCSGKVFFDLIAKRNE
                     LAREDVAIIRIAQLYPFPHDDFQANIDLYANAREVLWCQEEPGNQGAWHRIQHYLLRH
                     MRADQQLAYAMRASSASPAVGYLSLHLEQQKALVAAAFAPVTDNLVMPVQSSARKDG"
     gene            2745..3968
                     /gene="odhB"
                     /locus_tag="AC16O9_12920"
     CDS             2745..3968
                     /gene="odhB"
                     /locus_tag="AC16O9_12920"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR6517557.1"
                     /translation="MLVEVKVPQLSESVSEATLLAWHKKQGEAVKRDENLIDIETDKV
                     VLELPSPADGVLVSVLKGDKGTVVAGEVIAKIDTEAKASAGSSKVAASAAGPAAEAAK
                     TDLAPATSAAPAARKIAAEKGVDLASVQGTGRDGRITKGDVLLAVQPATAAPAARAPL
                     QQPPAPVSVEQLLAGRPEERVPMSRLRARIAERLVQSQSAAAILTTFNEVNMQPVIEL
                     RKKYQERFEKDHGVRLGFMSFFVKAAVHALKKYPIVNASIDGTDIVYHGYFDIGVAVG
                     SPRGLVVPIVRDADQLSFAAIEKKIAEFGKKAQDGKLALEDLTGGTFSISNGGVFGSM
                     LSTPIINPPQSAILGVHATKERPVVEDGQVVIRPMNYLALSYDHRIIDGREAVLTLVA
                     MKEALEDPARLALEI"
     gene            3972..5390
                     /gene="lpdA"
                     /locus_tag="AC16O9_12925"
     CDS             3972..5390
                     /gene="lpdA"
                     /locus_tag="AC16O9_12925"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6517558.1"
                     /translation="MAQAFDVVVIGGGPGGYIAAIRAAQLGLAVACVDQWQGAGGKPA
                     LGGTCTNVGCIPSKALLQSSENFEQAGHGFAEHGVKVKGLELDLAQMLKRKDKVVKQN
                     NDGIVYLFKKNKVAFFHGCGSLAASGKAGHYVAVGGQSLSAKHVVIATGSSARALPGA
                     EFDEKLILSNAGALALTEVPKRLGIVGAGVIGLELGSVWRRLGAEVTILEALPELLGA
                     ADQQIAKEAAKVFAKQGLDIRLGVKISKISSSKKTVTVQYTASDISSITLDCDKLIVS
                     IGRVPNTEGLNAAAAGLMLDEKGFVVVDEDCRTNLPNVWAVGDVVRGPMLAHKAEEEG
                     AAVAERIAGNKPHVDFNLVPWVIYTAPEIAWVGKTEQQLKADGVEYRAGTFPFSANGR
                     ARALGESAGLVKILAHAQTDEILGAHVMGPFAGELIAEAAVAMAFRGSAEDLGMICHA
                     HPTFSEAIKEAALAVDKRALNL"
     gene            5387..5626
                     /locus_tag="AC16O9_12930"
     CDS             5387..5626
                     /locus_tag="AC16O9_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517559.1"
                     /translation="MRAALAALCLALAGCYGTQIYPTLQEQRISLAPGDLEAGGVAFI
                     TPSTVTGQEQETQAVALVFADVLKRTARDLIARLA"
     gene            5647..5790
                     /locus_tag="AC16O9_12935"
     CDS             5647..5790
                     /locus_tag="AC16O9_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517560.1"
                     /translation="MHAIAEVPERGAPRKSIEGFASVTIRTMIPIGLSPALAAAARRA
                     PRA"
     gene            5787..6908
                     /gene="zapE"
                     /locus_tag="AC16O9_12940"
     CDS             5787..6908
                     /gene="zapE"
                     /locus_tag="AC16O9_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR6517561.1"
                     /translation="MSTGVLALYRQSLASRGFASDPSQQRAAEHLQQLYEEWTAYKAR
                     RNSALKRLIVRPALPRGVYLWGAVGRGKSFLMDAFYRCVPLVRKRRVHFHHFMREIHR
                     ELDALKGTEDPIAAVAARTARRYRLICFDEFHVSDIADAMILGRYLRQVMDRGVQFVM
                     TSNYHPDRLYPDGLQRARFAPAVALLKARLDVVEVDNGTDYRRLKMEKLEAYHVGAGG
                     EAALERIFDELKDVEEEKQPLDVEGRKIPYRRRAGGLVWFDFAALCGGPRSYADYVDL
                     AQRFHTVILSGVPRMSAKHADAARRFTWLVDVLYDDRVNLVLAAEAPPEALFTEGAAS
                     AEFQRTASRLHEMRSAEYLASERRRSGGKVQSLQADRGR"
     gene            6868..7863
                     /locus_tag="AC16O9_12945"
     CDS             6868..7863
                     /locus_tag="AC16O9_12945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acrylyl-CoA reductase family protein"
                     /protein_id="HIR6517562.1"
                     /translation="MEKFKAYKLTEVDKKIRAEFVECTLEDLDPGEVVVRVAYSDVNY
                     KDALAATGKGRILMRPSCIGGIDLAGTVVSSSDAQFVKGDAVLAVGHEIGVKHHGGYA
                     EYARIPAAWAVKLPKGMTLWDAMAFGTAGYTAGLAIVRMEHNGLKPANGPVLVNGATG
                     GVGSIAIAALARLGYSVTALTGKESESAWLKQLGARDILLRQNLELAKIRPLDKATWA
                     GAVDNLGGEVLAWMASTMKFNGVIASIGLAASPSLNTTVLPFILRGVSLLGINSTDCP
                     TPQLEREVWRRLATDMRPPMLKEMARTVPFADLPKVFDDFINAKVSRRIVIDMAS"
     gene            7869..8966
                     /locus_tag="AC16O9_12950"
     CDS             7869..8966
                     /locus_tag="AC16O9_12950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR6517563.1"
                     /translation="MAHKPGPGRPRVLVIDDSKFVRTTFRRILESSMDVREEVDGDAG
                     WRAIAGDAAIQLVFCDISMPNMDGFGVLRHVRGAADERIRQLPVIVISGDEDEATRRR
                     VREAGATDFISKSADATEVLSRIDNQLRLLRAKEDAKESRKAAQTATHDPLTGAFSLH
                     YLLTEGRKQFAYARRHGTPLSVVVFRIDSHRPIARKEGSEVAGQLLAKIARAIQGVLR
                     TEDALGRSGEDAFSVILAGASGPQAAAFARRLRDRLAGARIEFQGRALRIQASIGVAA
                     MGNDAAAGIEDLLQLASGRLTQAEPAVPPPARAAAGGLPPEVERALQVLEAIPADRMG
                     EAAGEILRRLAPLLRAAGKPQRAAPPVARAK"
     gene            8981..10879
                     /locus_tag="AC16O9_12955"
     CDS             8981..10879
                     /locus_tag="AC16O9_12955"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HIR6517564.1"
                     /translation="MSYRNFHKRSIAEREEFWREEARLIHWHKPFSRVLDYSHPPFAR
                     WFVGGETNLCHNALDRHLGARAAQKALIWISTEVDEEKHFTYGELHAEVNRVAAMMQA
                     LGVKRGDRVIIYMPMIPEACFAILACARIGAIHSVVFGGFAASSLAARIDDAKPALMI
                     TADGGSRMGKPVLYKALVDEALKLAKHAPQKVLLFRRGLDPNMPLTAGRDVDWQELAA
                     KHAGAQVPCAWLESNEPSYILYTSGTTGKPKGVQRDVGGYAVALASSMQRIFCGAPGE
                     TMFTTSDIGWVVGHSYIIYAPLLAGMTTIMYEGVPVRPDAGVWWKIVQEHKVAVMFSA
                     PTAVRVLKKHDVAHLKKYDLSTLKHLFLAGEPLDEPTHRWISESLGRPVVDHYWQTET
                     GWPLLSAHPGVEKTPLKMGSPSFPVYGYDVQLLHESTGQPVADGEKGVVTIRPPLPPG
                     CMSTVWGDDERFVKTYFTDFKNQQVYSTFDWGIRDKDGYYFILGRTDDVINVAGHRLG
                     TREIEEAVNMHPGIAECAVVGVADQLKGQLPMAFAVVKDPAAIATKEMRMKLEGEVMQ
                     TVDKQLGAIGRPGRVFFVTLLPKTRSGKVLRRSIQALAEKRDPGDLTTIEDPGALEQI
                     RQALGSAG"
     gene            complement(10876..12006)
                     /locus_tag="AC16O9_12960"
     CDS             complement(10876..12006)
                     /locus_tag="AC16O9_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6517565.1"
                     /translation="MVMGPSCGLVLADLGAEVIKVEPLEGDNTRRLMGAGAGFYPMFN
                     RNKKSLALDLKKAEGRQIALKLIDSADALTENFRPGALEKMGFGYEDLKQRNPRLVYC
                     TMKGFLSGPYEHRPALDEVVQMMGGLAYMTGLPDRPLRVGSSVNDIMGGMFAAIGILA
                     ALREREKTGQGQLVRSALYENTAFLVAQHMAQLAITGEEPPPMAVKKPAWGVYDIFPT
                     KDGDRLFVGVVTDTQWEVFCREFGADALAADTRLKTNGLRVKAREWLLPKLEEILKRY
                     TKAELEQHLEAIGLPFAPITRPRDLFDDPHLNAGGMVETKDPRGYAFKVPGLPLELGG
                     QRLGLRRQPPQVSQDARALLASLGYAPAKIDALLADKVVAAP"
     gene            complement(12035..12943)
                     /locus_tag="AC16O9_12965"
     CDS             complement(12035..12943)
                     /locus_tag="AC16O9_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6517566.1"
                     /translation="MRQVQICEVGPRDGLQNAKQLMPTETKKAWISALAAAGLNEIEV
                     GSFVPPKLIPAMADTGEVVKHALTLKGLKVVALAPNVKGFERAREAGAHKITFPVSAS
                     HQHSVSNVRMTPQQMVDEVRKAAAMRSGKTPEIEGAVSTAFGCTMQGNVPEDDVLRIA
                     VKLAEFCDCVALADTVGYANPAQVRRVFKRVKQEIGDKLEGAHFHNTRGLGLANALAA
                     YEEGVRSFDSSMGGLGGCPFAPGASGNVITEDLVFMFESMGVKTGIDFDKLLAARAIM
                     HGGVPDEPLYGHMALAGLPKGFRAAA"
     gene            complement(12969..14138)
                     /locus_tag="AC16O9_12970"
     CDS             complement(12969..14138)
                     /locus_tag="AC16O9_12970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517567.1"
                     /translation="MAGIRWPAAWAVFAGGLVCGAYVGKVPPALPEQRDALQLSLVQA
                     GFIATTFNAIGGLAGMLFGVLCDRFGHKRLGLTGLAVMCLAGLFGAAAWDFASLLASR
                     FFEGVGFMLYAVAGSALMAASAEDARGRSRVMGLWSAYMPSGGSAAILLAPLVLAAWG
                     WRGLWVAVSVAAAAAFLLAARHAPATRYGGVGSLKLALESLSQPASVALALLFAFYVA
                     QWTSVMIWLPTFLVEERGASAALASLLTAFMVLINVPGNLGGGWLLAHGVRRGPLILV
                     ACAVMVVTDIGMLSDLLPDGLRYASCLLFSMCAGVIPACIFSGVVAHAKTPEHIGTTN
                     GMVMQTSQAGQFFGPILLAWLASSFGGWGASLWAMLAFAAGGALCGYAILRIEAR"
     gene            complement(14138..15664)
                     /locus_tag="AC16O9_12975"
     CDS             complement(14138..15664)
                     /locus_tag="AC16O9_12975"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR6517568.1"
                     /translation="MTTIKQEDLVQSVADALQFISYYHPADYIRALGRAYEREQSTAA
                     KDAIAQILTNSRLCAEGHRPICQDTGIVNVFLKVGMDVRFEGFKGALADAVNEGVRRG
                     YTHPDNPLRASVLEDPQFARRNTRDNTPAVVNVEMVPGDKVEVDVAAKGGGSEAKAKF
                     AMLNPSDSVVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMAPIDMAEL
                     KARGPKDKYEELRIELYDKVNALGIGAQGLGGLSTVLDVKVAAYPTHAANKPIAMIPN
                     CAATRHAHFVLDGSGPAEFVPPSLDDWPKVTWSPSKDARRVDLDALTPAEVGAWKPGE
                     TLLLSGKLLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVGKEVVGPAGPT
                     TATRMDKFVEMMLAQTGLVAMIGKGERGPVAIEAIRKHKAASLIAVGGAAYLVAKAIR
                     KSKVLAFADLGMEAIYEFEVKDMPVTVAVDAKGVSVHQMGPQDWHKKIVELKIPVHAA
                     "
     gene            complement(15661..16275)
                     /locus_tag="AC16O9_12980"
     CDS             complement(15661..16275)
                     /locus_tag="AC16O9_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HIR6517569.1"
                     /translation="MTPLPGERALRPLKPLPNLIFASRWLQLPLYLGLIVAQGVYVWL
                     FWQELYYLLLAAFGNANALGHVIDAVTVEGVARPAKLTETVIMLVVLGLIDVVMISNL
                     LIMVIVGGYETFVSRLGLESHPDQPEWLSHVNASVLKVKLATAIIGISSIHLLKTFIN
                     ASAYDEKTIIAQVGIHITFLLSAMAIAASDRLMPQAPLRHGGRA"
     gene            16360..18330
                     /gene="acs"
                     /locus_tag="AC16O9_12985"
     CDS             16360..18330
                     /gene="acs"
                     /locus_tag="AC16O9_12985"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR6517570.1"
                     /translation="MSSTIESVLQETRVFPPAPEFVKQANVPGMEAYRKLCAEAEKDF
                     EGFWGRLAKEHLLWHKPFTHVLDESKAPFFRWFHDGELNASHNCLDRHLKTQPDKVAI
                     IFEADDGKVTRITYKALYHEVCKLANALKAHGVKTGDRILIYMPMSIQVVVAMQACAR
                     IGATHSVVFGGFSAKSLQERIVDAGATEVITADGQFRGGKEIPLKPVVDEAFAMGGCD
                     KVKNVIVYKRTGSKVPMQAPRDKWWDDVVNGQADTCEPTWVNAEHPLFILYTSGSTGK
                     PKGIQHSTGGYLLWCALTMKWVFDNKPDDVFWCTADVGWVTGHSYITYGPLAVGTTEV
                     MFEGVPTYPDAGRFWKIIQDHKVNVFYTAPTAIRSLIKAGSDLPKNYDLKSLRILGTV
                     GEPINPEAWMWYHETVGGGRCPIVDTWWQTETGGHLITPLPGATPTKPGSATFPLPGI
                     MADIVDETGRTVGRGKGGILVIKRPWPSMLRTIWGDPERYKKTYYPDDFKGKYYLAGD
                     GASMDDEGYYRIMGRIDDVLNVSGHRLGTMEVESALVANSKLVAEAAVVGRPDELTGE
                     AIVAFVVTKGPRPTGDEAKRIAKELRDWVGKEIGPIAKPKDIRFGDNLPKTRSGKIMR
                     RLLRAIAKGEEIKQDVSTLENPAILEQLAQKQ"
     gene            18369..18566
                     /locus_tag="AC16O9_12990"
     CDS             18369..18566
                     /locus_tag="AC16O9_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517571.1"
                     /translation="MGEYDVRGQRLAALCLLGLLLFNYPLLAVFNVPRMMFGIPVLYV
                     YFFLAWASLIGLMALVIERRG"
     gene            18587..21355
                     /locus_tag="AC16O9_12995"
     CDS             18587..21355
                     /locus_tag="AC16O9_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6517572.1"
                     /translation="MLQGWVVVLTSFAYIGLLFAIAYYADRRADAGRSLIDSPYVYSL
                     SLAVYATAWTFYGSVGRAAENGVGFLPIYIGPTLMIALWWIVMRKILRISKQNRITSL
                     ADFVSSRYGKSALLAGMVTVIAVIGIVPYISLQLKAISNTFAVVLQYPDIVMPAKLGT
                     VPVLQDTALFVALFLAAFTILFGTRHLDTSEHHEGMVAAIAFESLVKLVAFLAVGAWV
                     TWGIYNGFGDLFARAAAEPKLAAMLTPFDAVSGSYSSWVWLTILSMMAIMFLPRQFQV
                     TVIENKDEKHLNKAIWLFPLYMLAINVFVLPIAFGGLLHFPGGGVDADTFVLTLPMVE
                     KQELLALIVFIGGLSAATGMVIVETIALSTMVCNDLVMPVLLRMRALRLNERPDLSGL
                     LLGIRRGAIVAVLLLGYLYFRVAGEAYALVAIGLISFAAVAQFAPAALGGIFWKGGTR
                     LGALAGLAAGFTVWFYTLLLPAFARSGWLPIGFLEEGPWGIALLRPLELFGLGGLDQI
                     THAMIWSMAANIGAYVGVSLLRAPGPEELRQASMFVDVFTRPAPVGGAGFWRGKAPAA
                     ELQNLLARFVGAGAAQEEFAEYARTRGLEPSALGPEADADLVYFAETHLAGVIGAASA
                     HIMVASVVKEEALTLDEVRAILDEASQVVVYSRQLEQKSRELEAATAELRRANERLQE
                     LDRMKDDFISTVTHELRTPLTSIRAFSEILRDDPKLALPQRTKFLDIIAKETERLTRL
                     INQVLDLAKLESGAAEWHTKSVDMREIVAETVTGMSEVFRERAVRLELRMPGIVPPVH
                     ADPDRVKQVLLNLLSNAAKFCQADRGRVEVSLLEERDAVRVDVKDNGPGISLADQEII
                     FEKFRQVGNALTDKPQGTGLGLHICRQIVERLGGRLWVESRVGEGACFSFTLPVPRHR
                     HTLSARTA"
     gene            21396..21770
                     /locus_tag="AC16O9_13000"
     CDS             21396..21770
                     /locus_tag="AC16O9_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6517573.1"
                     /translation="MSKKVLLVDDEPNIVAALEFLLEKNGYDVKVAANGVEAIAQLDA
                     FRPDLVLLDVMVPKLSGYEVCQRMRAEPKWRDIKIVMLSAKGREVEVNKGMSLGADLY
                     VTKPFSSAELVATIQQLLEHRA"
     gene            21767..23911
                     /locus_tag="AC16O9_13005"
     CDS             21767..23911
                     /locus_tag="AC16O9_13005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6517574.1"
                     /translation="MKARGKFALWVAVLFAAGVGVPLATALAVVAALAAPDRDVVLGI
                     LEPRMPLIIFGALALFALCAGTVRWLFASYVEPAAALAELTRGARAAQGDTPVASDGA
                     GELVRLAREIDQLAQETRRLRAVSDARVQAALADLEEERNRLAALMSELVEGVLVCNA
                     EGRIMLYNEQARALLRNDASPAAAAPVGLGRSIFTLIDREQVAHAIEKLEQSRQRGAA
                     APRTRFVAPAGPARLVKFEAAPYRGAGGELAGIVFTLSDVTGLLEREAQHLATLQSIA
                     THARAPVANIRAAAENLARYPDMEAERQRRFVDIVVDESQTLSHSIRRALQTYGEALK
                     ASLILEDMQALDLLRVAEGRIRALAGVEAALEPIDESLWLTVDSFSFVHALCFLAARL
                     KEDYGVHRIRFAARAAERFVELDLGWRGAVVGHDALTQWESEPLRAGAQESPLTLRDV
                     IERHGGELWLQREGPAGQRDACFRFLLPAGEPVAAPVRAEASIQSRPEFYDFDLFAHA
                     GASLDLRERPLAELAYTVFDLETTGLAPSGGDEIISIGAIRVVNGRLLRSEVFEKLVD
                     PRRPLNPESAKVHGIDARMLEGQPKIEEVLPAFHRFCEDTILVAHNAAFDMRFLELKE
                     KAARVRFEQPVLDTLLLSAVAQPAQQDHRLEAIAARLGLPVIGRHTALGDALLTGEAF
                     LRLLTLLREQGIATLGQALDASRETYYARLRY"
     gene            23929..25410
                     /locus_tag="AC16O9_13010"
     CDS             23929..25410
                     /locus_tag="AC16O9_13010"
                     /inference="COORDINATES: protein motif:HMM:NF021824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HIR6517575.1"
                     /translation="MAEPRPDLRERMAGERQAFALPLQALVRRAVITCEEQRSVAEAA
                     RIMQDSDVGSVIIVDAHSRPAGIFTSRDLVQVASAGGLERRVTDFMSRDLVTLPPHAF
                     AYEAALEMTERRIRHILVTEGGRLLGVVSERDLFALQRLGLGELTMEIRLADSIETLA
                     GLAEQVRGLATLLIEQGTAPEPLTLFISVLNDRLTRRVIEVVRRRHELNRIRWTWLAF
                     GSEGRLEQTFSTDQDNGLVFAAPEGVAPDAARAQLIPFAHEVNRALDACGFPLCEGNI
                     MASNPALCLTLEEWRAKMTGWLNVTSPQALLDAAICLDLRPIHGDEVLATELRGWIVE
                     RVRGHAPFLRLLAQVATRMRPARSRFGALVTTDAPGAPRTVNLKANGARIFVDAARVL
                     ALASGVRQTNTADRLRAYREVRDQPEAETGALVDAFHFIQGLRLRIQAGARKARAGGR
                     GAGEDLANRIDPSALNAFERSCLKEALRLARELQRRLELDFLV"
     gene            25407..26048
                     /locus_tag="AC16O9_13015"
     CDS             25407..26048
                     /locus_tag="AC16O9_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6517576.1"
                     /translation="MRHGPDDERAAQQTRPTAYIVDDDESIRTLWRWLLESNGMAVEA
                     FATAKEFIGAYQPGGPACLVLDLRLPGMSGLELQEYLREREIAIPIVFVTGHGDVPTA
                     VSAIKGGAVDFIQKPFGYREVLAIIQRAFERDAEVREQRTRRSQAAERLATLTERERE
                     VMQRVVEGKFNKVIADELGISVKTVEFHRAKVMEKMGADSVAGLVQLMMQHAS"
     gene            complement(26160..26840)
                     /locus_tag="AC16O9_13020"
     CDS             complement(26160..26840)
                     /locus_tag="AC16O9_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517577.1"
                     /translation="MSIMAAQGRTLDNAAVHPFLARRLEALRRWYRRWRRREPIRDVG
                     ALRAFLQSRASHVAQMTLYGYLRTRAGTRFPELFENDAFVASMNIAKWHVWLACLSDL
                     AVHVGGMLRCASGASPDEIGSVMQRLVDQLLLETGTPAEAGPEFAAHAQRVRARLALC
                     AWNATEGEVAFAESPDALVYWAPIVDDLKALDADIVRNSVRFRWHEVQRDLSHNLDPV
                     AVLSSAAQ"
     gene            26922..27185
                     /locus_tag="AC16O9_13025"
     CDS             26922..27185
                     /locus_tag="AC16O9_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HIR6517578.1"
                     /translation="MATEEANRVVHWQRTRTLMIWHMIIWFIFSFLVHWFATNLNAIT
                     FMQFPLGFYMAAQGSLAVFVVQLFVFTWQQHKIDVECGMAETE"
     gene            27200..29038
                     /locus_tag="AC16O9_13030"
     CDS             27200..29038
                     /locus_tag="AC16O9_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6517579.1"
                     /translation="MAMFGDFSSKGGFIENLPKIYGLYTGGFVVFVGLMAIAEQMGVK
                     EDTIGLMFVGFTIAIYALIGWLSRTMQLDAYYVAGRQVPTVFNGMATAADWMSGASFV
                     AMAGGIYLGGHPYLAFVVGWTGGYVLVAVLMAPYLRKFGCYTVPDFIGTRYGGNFARL
                     CAVLVLVVASFTYVTAQINATGTIASRALLIPFEVGIWIGLAGILLCSMLGGMRAVTW
                     TQVAQYIVLIIAYLVPVIWMSNKQGFGVIPQFVYGDAVQRIAELEQMHQVGVLKPSET
                     VAGLSALVTKHNAPADSFVAAWRFLTLTAAMMIGTASLPHILMRYFTTPSVKAARASV
                     AWSLFFIFLLYFTAPALATFTKLQMLDPNLATSVIGKSLAEVNAHVWVQKWAAVGGLK
                     VIDSNGDGIVQVNEFFIRGDIVVLATPEIAGLPYVISGLVAAGGMAAAMSTADGLLLA
                     IANALSHDLYYKMIDPKADVKRRLIVARVLLVVIGAAGALVASLRLTGILGAVAWAFC
                     FAMSGLFFPLVLGIWWKRATSAGAIAGMLGGLAAGSWYLYMVYAGGMAPWWGMDHLRF
                     GIVGAAVSLVLMVVVSLLTPAPDQATQDMVDEVRIPKGQTILGTTH"
     gene            29133..29489
                     /locus_tag="AC16O9_13035"
     CDS             29133..29489
                     /locus_tag="AC16O9_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517580.1"
                     /translation="MDFPFPLWVIILDYLLGVVMWTLIGRTAMRIFLPENSSFFFMRF
                     FVRSTDPLLKLFRPITPAFLIQPLVPLYVAWFFFLVRFYVMPLLLGYTVMGMLSFPLE
                     GEIARSISRLMQKIGY"
     gene            29560..29650
                     /locus_tag="AC16O9_13040"
     tRNA            29560..29650
                     /locus_tag="AC16O9_13040"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29594..29596,aa:Ser,seq:gga)
     gene            29664..29762
                     /gene="ffs"
                     /locus_tag="AC16O9_13045"
     ncRNA           29664..29762
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16O9_13045"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            29822..31375
                     /gene="dnaX"
                     /locus_tag="AC16O9_13050"
     CDS             29822..31375
                     /gene="dnaX"
                     /locus_tag="AC16O9_13050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR6517581.1"
                     /translation="MSYQVLARKWRPRNFASLVGQEHVVRALRHALEQKRLHHAYLFT
                     GTRGVGKTTLARILAKCLNCETGITAEPCGRCSACEEIDGGRFVDLLEVDAATNTRVD
                     EMRQLLDTAQYAPTRGRFKVYVIDEVHMLSNSAFNAMLKTLEEPPEHLKFVLATTDPQ
                     KIPVTVLSRCLQFNLKQMPPAAIAGHVAELLKAESIAFEPDALQLIGRAASGSMRDAL
                     SLLDQAIAHGGGRVATEGVRDMLGAVDDTYLLRLLGAVATSDGAQLMAIADEMQARSL
                     SFDSALQDLAGLLLELAVMQAAADAVDADRPGYEARAALAQQLDAETVQLCYQIALQG
                     RDDLPLAPDEHAGFVMTLLRMLAFRPEAAAAEERPSGAAATAAPAAARAAQRKEFDGD
                     WPALVRRLPVGGAAGELARNSVLRRHSAGAIELIVPKAMAHLAERGYQEKLKAVLEQH
                     FGVPMALKVVAGEAPGETAASREAGEREARRAEAARAVQGDRFVQDLVDIFDAKVVGS
                     SIKANGNKD"
     gene            31390..31713
                     /locus_tag="AC16O9_13055"
     CDS             31390..31713
                     /locus_tag="AC16O9_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6517582.1"
                     /translation="MRGNIGQLMKQAQMMQENMRRMQEQLAAMEVEGQAASGKVKVVM
                     TCKHEVRRVSIDPELAAEDRELLEDLMIAAFNDAARQVDALVSEKMGGMTAGLGLPAG
                     FKLPF"
     gene            31733..32329
                     /gene="recR"
                     /locus_tag="AC16O9_13060"
     CDS             31733..32329
                     /gene="recR"
                     /locus_tag="AC16O9_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6517583.1"
                     /translation="MPSAKGLERLVEALRVLPGVGPRSAQRMAYYLLQRDRGGAEQLA
                     HALSEALARVRHCALCNNYTEDEVCALCRSPRRDRTQLCVVETPADLAMVEQTSAYAG
                     MYFVLMGRLSPLDGVGPRDIGLEKLLARATEGDVAEVVLATNFTNEGEATAHYVAELL
                     RNRNVRVSRIARGVPLGGELEYVDAGTLSQALLDRRGM"
     gene            32408..32484
                     /locus_tag="AC16O9_13065"
     tRNA            32408..32484
                     /locus_tag="AC16O9_13065"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32442..32444,aa:Met,seq:cat)
     gene            32657..>33816
                     /locus_tag="AC16O9_13070"
     rRNA            32657..>33816
                     /locus_tag="AC16O9_13070"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5670 a        11017 c        11721 g         5408 t
ORIGIN      
        1 agcgcctgca gcgcgtgccg gggtccggcg gcgcggtgcg cgacgtggcg cacgcgccga
       61 tcgtcgaggc cttcgcgcaa cgggcgaagc gcgggccggc gcggcacgcc gcgcagactg
      121 cgcccgatcg caagcaggtc tcggtgatcc agctggtcgc ggagtaccgc ttccgcggct
      181 gcctggtggc ggacctcgat cccctgaggc gcctgcccaa gccgcacatc gtcgagctcg
      241 agccgggcta ttacgacctg ggcgaggccg acctcgacac gccgttcaat accggcacct
      301 acatgggcgc cgagcaggcg acgctgcgcg aaatcatcca gcacctgagg gacacctact
      361 gcggcacgct cggcgtcgag tacatgtacc tctccagccg cgccgagaag cgctggatcc
      421 aggagcgcct cgagccgatc cgttcgcggc cgagcttctc ggcggaggag aagaagcaca
      481 tcctcgagcg cctcagcgcc gccgagggcc tggagcgcta cctgcacacg cgctacgtcg
      541 gccagaagcg cttctcgctc gagggcggcg acacgctgat cccggtgctc gaccacttgc
      601 tgcagcgcgc gggcgccgcg ggcgtgcagg agctggtgct cggcatggcg caccgcggcc
      661 gcctcaacgt cctcgtgaac atcctcggca agatgccgaa gaccctgttc cgcgagttcg
      721 agggcgagca cgacgcgtcg gtgctcgccg gcgacgtcaa gtaccaccag ggcttttcct
      781 cggacatcaa cacgccgggc gggccgatgc atctgacgct cgctttcaac ccctcgcacc
      841 tggagatcgt caacccggtg gtcgagggct cggtgcgcgc gcggcagcac cgccgcaagg
      901 accgcgacgg ctcgcaggtg ctgccggtcc tgatccacgg cgacgccgcg gtggcggggc
      961 agggcgtggt ccaggagacg ctcaacctct cgcagacgcg cggctacggc accggcggca
     1021 cggtgcacat cgtcatcaac aaccagatcg gcttcaccac ctcggacgcg cgcgacgtgc
     1081 gctcgacgct gtactgcacc gacgtcatga agatgatcga ggcgccgatc tttcacgtca
     1141 acggcgacga tcccgaggcc gcggtgatgg cgatcgagat ggcgctcgac ttccgcaccc
     1201 agttcaggaa ggacgtggtg gtggacctgg tctgtttccg caagctcggc cacaacgagc
     1261 aggacgagcc catggtgacg cagccgctga tgtacaagat catcaacaag catccgggca
     1321 cccgcaagct ctacgccgag cggttgctcg cgcagggggt gatcgagccc gacgaggccg
     1381 acaaggtagc ggccaattac cgacaggcgc tggaaggggg cttccacacc aacaagacga
     1441 tcctgtcgaa ctacaagccg ccgttcgcgg tcgactggtc gaaattcagg ggcacgaagt
     1501 ggaacgagca cgacgacacg cgcatcccgc tcgcggcgct gcaggcgctc gccgagcgcc
     1561 tgaccgcggt gccgcccaac ttcaagctgc acccccgcgt cgagaagatc atcgccgacc
     1621 ggcgcctgat gggacagggc aagatcccgg tggactgggg catggcggag aacctcgcct
     1681 acgcctcgct gctgaaggac ggctacgccg tgcgcatctc ggggcaggac agcggacgcg
     1741 gcacgttctt ccaccgccac gccgtgctgc acgaccagaa tcgcgagagg ttcgacgcgg
     1801 gcacctacgt tccgctgcag catatcgcgc cgaaccaggg cgatttcgtg gtcatcgact
     1861 cggtgctgtc ggaggaggcg gtgctcggct tcgagtacgg ctactccacc tcggagccca
     1921 atgagctcgt ggtatgggaa gcgcagttcg gcgacttcat gaacggcgcc caggtggtca
     1981 tcgaccagtt cattgcctcg ggcgaggtca agtggggccg catctgcggc atggtgatga
     2041 tgctgccgca cggctacgag ggccagggcc cggagcattc ctcgggccgc atcgaacggt
     2101 tcctccagct gtgcgccgat tacaacctcc aggtgtgcat gccggcgacg ccggtgcaac
     2161 tcttcttcct cctgcgccgg cagatgatcc ggccgtaccg caagccgctc gtcatcttca
     2221 cgccgaagag cctgctgcgg cacaaggaat ccgtctcgcc gatcagcgag ttcgcggaaa
     2281 gcaagttcaa gccggtgaac gcggaatgga acgaagacct gaagcccgac aaggtgaccc
     2341 gggtcatctt ctgcagcggc aaggtgttct tcgacctgat cgcgaagcgc aacgaactcg
     2401 cgcgcgagga cgtggcgatc atccgcatcg cgcagctcta tccgttcccg cacgacgact
     2461 tccaggccaa catcgacctc tacgccaacg cccgcgaggt gctgtggtgc caggaggagc
     2521 ccggcaacca gggggcatgg caccgcatcc agcactacct gctacggcac atgcgcgccg
     2581 accagcagct ggcttacgcc atgcgcgcct cttcggcctc tccggcggtc ggctacttgt
     2641 ccctgcacct ggagcagcag aaggcgctgg tcgcggcggc cttcgctccg gtcaccgaca
     2701 acctggtgat gccggtgcag agctcggcgc gaaaggacgg ctagatgctg gtcgaagtga
     2761 aggtgccgca gctctccgag tcggtatccg aggcgacgct gctcgcctgg cacaagaagc
     2821 agggcgaggc ggtgaagcgc gacgagaacc tgatcgacat cgagaccgac aaggtggtgc
     2881 tcgagctgcc cagcccggca gacggcgtcc tggtgagcgt gctgaagggc gacaagggca
     2941 ccgtggtggc gggcgaggtg atcgcgaaga tcgataccga ggcgaaggcc tctgcgggca
     3001 gttccaaggt cgcggcttct gccgccggtc ctgcggcaga agccgcgaaa acggatctcg
     3061 cgcccgcgac gagcgccgcc cccgccgcca ggaaaatcgc cgccgagaag ggcgtcgatc
     3121 tggcgagtgt tcaaggaacg ggcagggacg gccgcatcac caaaggcgac gtcctcctgg
     3181 cggtccagcc cgccaccgcg gcgccggcgg caagagctcc gctgcagcag ccgcccgcgc
     3241 ccgtcagcgt cgagcagctg ctggcggggc gcccggaaga gcgcgtgccg atgtcgcggc
     3301 tgcgcgcacg catcgccgag cgcctggtgc agtcgcagtc cgcggcggcg atcctcacca
     3361 ccttcaacga agtgaacatg cagcccgtca tcgagctgcg caagaagtac caggagcgct
     3421 tcgaaaagga ccacggcgtg cgcctgggct tcatgtcttt cttcgtcaag gcggcagtgc
     3481 acgcgctgaa gaagtacccg atcgtgaacg cctcgatcga cggcacggac atcgtctacc
     3541 acggctattt cgacatcggc gtcgcggtcg gcagcccgcg cgggctggtg gtgccgatcg
     3601 tgcgcgacgc cgaccagctc tccttcgccg ccatcgagaa gaagatcgcc gagttcggca
     3661 agaaggccca ggacgggaag ctcgccctgg aggaccttac cggcggcacc ttctccattt
     3721 ccaacggcgg cgtgttcggc tcgatgctct ccacgcccat catcaacccg ccgcaatccg
     3781 cgatcctcgg cgtgcacgcc accaaggagc gccccgtggt ggaggatggc caggtcgtga
     3841 tccggccgat gaactacctg gccctctctt acgaccaccg catcatcgac gggcgcgagg
     3901 cggtgctcac gctggtcgcc atgaaggaag cgctggaaga cccggcgcgg ctcgcgctcg
     3961 agatctgagc ggtggcgcaa gccttcgacg tagtggtgat cgggggtggc cccggcggct
     4021 acatcgccgc gatccgcgcc gcgcagctcg gcctggccgt ggcctgcgtc gaccaatggc
     4081 aaggcgccgg cggcaagccg gcgctcggcg gcacctgcac caacgtcggc tgcattccgt
     4141 cgaaggcgct gctgcagtcc tcggagaact tcgagcaggc cgggcacggc ttcgccgagc
     4201 acggcgtgaa agtgaagggg ctcgagctcg acctcgcgca gatgctcaag cgcaaggaca
     4261 aggtggtgaa gcagaacaac gacggcatcg tctacctgtt caagaagaac aaggtcgcct
     4321 tcttccacgg gtgcggcagc ctggcggcct ccggcaaggc ggggcattac gtcgccgtgg
     4381 gcgggcagtc gctcagcgcc aagcacgtcg tcatcgccac cgggtcgagc gcccgcgccc
     4441 tgccaggcgc ggaattcgac gaaaagctga tcctcagcaa cgccggggcg ctcgccctga
     4501 ccgaggtgcc caagcgcttg gggatcgtcg gggcgggtgt catcggcctc gagctcggca
     4561 gcgtctggcg ccggctcggt gccgaagtga cgatcctcga ggcgctgccc gagttgctcg
     4621 gtgcggccga tcagcagatc gcgaaggagg ccgccaaggt gttcgccaag caggggctcg
     4681 acatccgcct cggcgtcaag atttcaaaga tctcatcatc aaagaaaacc gttacggttc
     4741 aatatactgc aagtgatatc tcaagcataa ccttggattg cgacaagctc atcgtctcga
     4801 tcggccgggt accgaacacc gaggggctga acgcggccgc ggcgggcctc atgctcgatg
     4861 agaagggctt cgtcgtggtg gacgaggact gccgcaccaa cctgcccaac gtgtgggcgg
     4921 tcggggacgt ggtgcgcggc ccgatgctgg cgcacaaggc cgaggaggag ggcgcggccg
     4981 tggccgagcg catcgccggc aacaagccgc acgtggactt caacctcgtg ccctgggtga
     5041 tctacaccgc gcccgagatc gcctgggtgg gcaagaccga gcagcagctg aaggcggacg
     5101 gggtggagta ccgcgccggg acgttcccgt tctcggccaa cggccgcgcg cgcgcgctgg
     5161 gcgagtcggc cgggctggtg aagatcctcg cgcacgcgca gaccgacgag atcctcggcg
     5221 cgcacgtgat ggggccgttt gccggcgaat tgatcgccga ggcggcggtg gcgatggcgt
     5281 tccgcggctc cgccgaggac ctcggcatga tctgccacgc gcatcccacg ttctcggagg
     5341 cgattaagga agccgccctg gcggtcgaca agcgcgcgct caacctatga gggcggcgct
     5401 cgcggccctg tgcctggccc tcgcgggctg ctacggcacg cagatctacc ccacgctgca
     5461 ggagcagcgc atttcgctcg cgccgggcga cctcgaggcg ggcggcgtcg cgttcatcac
     5521 gccctccacc gtgaccgggc aggagcagga gacgcaggcg gtggcgctgg tcttcgccga
     5581 cgtgctgaag cgcacggcgc gggacctgat tgcccggcta gcgtagtgcg ccctggcaac
     5641 gccgctatgc acgccatcgc cgaggtgccc gagcgcggcg cgccacgcaa gagcatcgag
     5701 ggcttcgcat cggttacgat ccgcacgatg attccgatcg gcctgtcgcc ggcgctggcg
     5761 gccgccgcgc ggcgcgcgcc gcgcgcttga gtaccggcgt cctcgcgctg taccggcaga
     5821 gcctcgcgag ccggggcttt gcctccgacc cctcgcagca gcgcgccgcc gagcacctgc
     5881 agcaactata cgaggagtgg actgcctaca aggcgcgccg caattcggcg ctgaagcgcc
     5941 tgatcgtcag gccggcgctg cccaggggcg tgtacctgtg gggcgccgtg ggtcgcggca
     6001 agagcttcct gatggacgcg ttctaccgct gcgtgccgct ggtgcgaaag cgccgcgtgc
     6061 atttccacca cttcatgcgc gaaatccacc gcgagctcga tgcgctgaag ggcaccgagg
     6121 acccgatcgc cgccgtcgcc gcgcgcacgg caaggcgcta ccgcctgatc tgcttcgacg
     6181 agttccacgt ctccgacatc gccgacgcca tgatcctcgg gcgctacctg cggcaggtga
     6241 tggaccgggg tgtgcagttc gtgatgacca gcaactacca ccccgaccgc ctttatcccg
     6301 acggcctgca gcgcgcgcgt ttcgcgcccg ccgttgcgct gctcaaggca cgcctcgacg
     6361 tggtcgaggt ggacaacggc accgactacc ggcgcctgaa gatggagaag ctggaggcct
     6421 accacgtggg cgcgggcggc gaggcggcgc tggagcgcat cttcgacgag ctgaaggacg
     6481 tcgaggaaga gaagcagccg ctcgacgtcg aagggaggaa aattccctac cgccggcgcg
     6541 caggcgggct ggtatggttc gatttcgccg cgctgtgcgg cgggccgcgc tcgtacgcgg
     6601 actacgtgga cctggcgcag cgcttccata cggtgatcct gtcgggggtg ccgcgcatgt
     6661 cggcgaagca cgccgacgcc gcccggcgct tcacctggct ggtggatgtg ctttacgatg
     6721 accgggtgaa cctggtgctc gccgcagagg cgccgccgga ggcgctgttc accgagggcg
     6781 ccgcgagcgc cgagttccag cgcacggcga gccggctgca cgagatgcgc tcggcggagt
     6841 acctggcgag cgaacggagg aggagcggtg gaaaagttca aagcctacaa gctgaccgag
     6901 gtcgataaga agatccgcgc cgagttcgtc gagtgcacgc tcgaggacct cgaccccggc
     6961 gaagtcgtgg tgcgcgtcgc ctactcggac gtcaactaca aggacgcgct ggcggccacg
     7021 ggcaagggca ggatcctgat gcggccctcg tgcatcggcg gcatcgacct cgccggcacc
     7081 gtggtgtcct cgagcgacgc gcaattcgtc aagggcgacg cggtgctcgc ggtcggccat
     7141 gagatcggcg tcaagcacca cggcggctac gccgagtacg cacgcatccc ggctgcctgg
     7201 gcagtgaagc tgcccaaggg gatgacgctt tgggacgcga tggcgttcgg caccgcgggc
     7261 tataccgcgg ggctggcgat cgtgcgcatg gagcacaacg gcctcaagcc cgccaacggc
     7321 cccgtgctgg tcaacggtgc gaccggcggg gtcggctcga tcgccatcgc cgcgctcgcc
     7381 aggctgggct actcggtgac ggccctgacg ggcaaggaga gcgagtcggc ttggctgaag
     7441 cagctcgggg caagagacat cctgctgcgc cagaacctcg agctcgccaa gatccggccg
     7501 ctcgacaagg cgacctgggc gggcgcggtc gacaacctgg gcggcgaggt gctcgcctgg
     7561 atggcgagca ccatgaagtt caacggcgtc atcgcctcga tcggactggc cgccagcccg
     7621 agcctcaata cgacggtgct gcccttcatc ctgcgcgggg tgagtctgct cggcatcaat
     7681 tcgaccgact gcccgacgcc ccagctcgag cgcgaagtgt ggcggcgcct ggccaccgac
     7741 atgcgcccgc cgatgctgaa ggagatggcg cgcaccgtgc cgttcgccga tctgccgaag
     7801 gtgttcgacg atttcatcaa cgccaaggtc tcccggcgga tcgtcatcga catggcctcc
     7861 tgagcgccat ggcgcacaag cccgggccgg ggcgtccgcg agtgctggtg atcgacgatt
     7921 cgaagttcgt ccgcaccacg ttccggcgca tcctcgagtc gagcatggac gtgcgcgaag
     7981 aggtcgacgg cgatgcgggg tggcgggcga tcgcgggcga tgccgccatc cagctggtgt
     8041 tctgcgatat ctcgatgccc aacatggatg gcttcggcgt cctgcggcac gtgcgcggcg
     8101 cggccgacga gcgcatccgg cagctcccgg tgatcgtcat ctccggcgac gaggacgagg
     8161 ccacgcgccg gcgcgtgcgc gaggccggcg ccaccgattt catctcgaag agcgccgatg
     8221 ccaccgaggt gctgtcgcgc atcgacaacc agctgcggct gctgcgggcg aaggaagacg
     8281 cgaaggagag ccgcaaagcc gcgcagactg ccacgcacga cccgctgacc ggtgctttct
     8341 cgctgcacta cctgctcacc gaaggccgca agcaattcgc gtacgcgcgc cgccatggca
     8401 ccccgctgtc ggtggtcgtc ttccgcatcg actcgcaccg cccgatcgca cgcaaggagg
     8461 gcagcgaggt cgccggccag ctgctggcga agatcgcgcg ggccattcag ggcgtgctgc
     8521 ggaccgagga cgcgctgggg cgctcgggcg aggatgcgtt cagcgtgatc ctggcgggcg
     8581 cgtccggacc ccaggcggcc gcgttcgccc ggcgcctgcg cgatcggctc gccggtgcga
     8641 gaatcgaatt ccagggccgt gcgctccgca tccaggccag catcggcgtt gccgcaatgg
     8701 gcaacgacgc cgcagccggc atcgaggatc tgctgcagct cgcctcgggc aggctgacgc
     8761 aagcggagcc cgcggtgccg ccgcccgcga gggcagcggc gggggggctg ccgcccgaag
     8821 tcgagcgcgc actgcaggtg ctcgaggcca tccccgcgga ccgcatgggc gaggcagcgg
     8881 gcgagatcct gcgtcgcctg gcgccgctgc tgcgtgcggc cggcaagccg cagcgcgccg
     8941 cgccgcccgt agcacgtgca aaatagcgca gggaggaacc gtgtcctatc gcaatttcca
     9001 caagcgatcc atcgccgagc gcgaagagtt ctggcgcgag gaagcgaggc tgatccactg
     9061 gcacaagccg ttctccaggg tgctcgacta ttcgcatccg ccgttcgccc gatggttcgt
     9121 tggcggcgaa accaacctgt gccataacgc gctcgaccgg cacctggggg cgcgcgcagc
     9181 gcagaaggcg ctgatctgga tctccaccga ggtggacgag gaaaagcatt tcacttacgg
     9241 cgagctgcac gccgaggtga accgcgtggc cgccatgatg caggcgctcg gcgtgaagcg
     9301 cggcgaccgc gtcatcatct acatgccgat gattcccgag gcgtgctttg cgattctcgc
     9361 ctgcgcgcgc atcggcgcca tccactcggt ggtgttcggc ggctttgccg cctccagcct
     9421 cgccgcgcgc atcgacgacg cgaagccggc gctgatgatc accgccgacg gcggcagccg
     9481 catgggcaag ccggtgctct acaaggcgct ggtggatgag gcattgaagc tcgccaagca
     9541 cgcgccgcag aaggtgctgc tctttcgccg cgggctggac ccgaacatgc cgctcaccgc
     9601 ggggcgcgac gtcgactggc aggagctcgc ggcgaagcac gcgggggcgc aggtgccctg
     9661 cgcgtggctg gagtcgaacg agccctcgta catcctttac acctcgggca ccacgggcaa
     9721 gcccaagggc gtgcagcgcg acgtcggcgg ctacgccgtt gcgctcgcct cgtccatgca
     9781 gcgcatcttc tgcggcgccc ctggcgagac catgttcacc acctccgaca tcggttgggt
     9841 ggtggggcac tcatacatca tctacgcgcc gctgctcgcc ggcatgacca ccatcatgta
     9901 cgagggcgtg ccagtgcggc ccgatgccgg cgtgtggtgg aagatcgtgc aggagcacaa
     9961 ggtggcggtg atgttctcgg cgccgaccgc ggtgcgcgtg ctgaagaagc acgacgtggc
    10021 acacctgaag aagtacgacc tgtccacgct caagcacctg ttcctcgccg gcgagccgct
    10081 cgacgagccg acgcaccgct ggatttccga gtcgctgggc cggccggtgg tcgaccacta
    10141 ctggcagacc gagaccggct ggcccctgct ctcggcgcat ccgggcgtgg agaagacgcc
    10201 gctgaagatg ggctcgccga gctttcccgt gtacggctac gacgtgcagc tgctgcacga
    10261 atcgaccggc cagccggtcg cggacggcga gaagggcgta gtgaccatca ggccgccgct
    10321 gccgccgggc tgcatgagca cggtgtgggg ggacgacgag cgcttcgtga aaacctactt
    10381 caccgatttc aagaaccagc aggtctactc gaccttcgac tggggtatcc gcgacaagga
    10441 cggctactac ttcatcctcg ggcgcaccga cgacgtgatc aacgtcgccg ggcatcgcct
    10501 cggcacgcgc gagatcgagg aggcggtgaa catgcatccg ggcatcgccg agtgcgcggt
    10561 agtgggcgtt gccgaccagc tgaagggcca gctgccgatg gcgttcgcgg tggtcaaaga
    10621 tcccgccgcc atcgccacga aagagatgcg catgaagctc gagggcgagg tgatgcagac
    10681 ggtggacaag cagctcggcg ccatcggtcg gcccgggcgg gtgttcttcg tcacgctgct
    10741 gcccaaaacg cgctccggca aggtgctgcg ccgctcgatc caggcgctcg ccgagaagcg
    10801 cgacccgggc gacctgacga cgatcgagga cccgggcgcg ctggagcaga tccggcaggc
    10861 gctcggttcg gcgggctagg gggcggcgac caccttgtcg gcgagcagcg cgtcgatctt
    10921 cgcgggggcg tagccgagcg aggcgaggag cgcgcgcgcg tcctgcgaca cctgcggcgg
    10981 ctggcggcgc aggccgaggc gctgtccgcc gagctcgagg ggcagcccgg gcaccttgaa
    11041 cgcgtagccc cgcggatcct tcgtctccac catgccgccg gcgttgaggt gcgggtcgtc
    11101 gaacaggtcg cgcggccgcg tgatcggcgc gaacggcagg ccgatcgcct cgaggtgctg
    11161 ctcaagctcg gccttcgtgt agcgcttgag gatctcctcc agcttcggca ggagccactc
    11221 gcgcgccttg acgcgcaggc cgttggtctt caggcgcgtg tccgcggcga gcgcgtctgc
    11281 gccgaactcg cggcagaaca cctcccactg cgtgtccgtc accacgccga cgaagaggcg
    11341 gtcaccgtcc ttggtcggga agatgtcgta gaccccccac gcgggcttct tcaccgccat
    11401 cggcggcggc tcctcgcccg tgatggcgag ctgtgccatg tgctgcgcga cgaggaaagc
    11461 ggtgttctcg tagagcgcac tgcgcacgag ctggccctgg cccgtcttct cgcgctcgcg
    11521 cagcgcggcg aggatgccga tggcggcgaa catgccgccc atgatgtcgt tcaccgacga
    11581 gcccacgcgc agcggccggt cgggcagccc ggtcatatag gcaagcccgc ccatcatctg
    11641 caccacctcg tcgagcgcgg ggcggtgctc gtagggcccg gagaggaagc ccttcatcgt
    11701 gcagtagacg aggcgcgggt tgcgctgctt caggtcctcg tagccgaagc ccatcttctc
    11761 cagcgcaccg gggcggaagt tctcggtcaa ggcgtcggcg ctgtcgatca gcttcagcgc
    11821 gatctgtcgg ccctcggcct tcttcaggtc cagggcgagg ctcttcttgt tgcggttgaa
    11881 catcgggtag aagcccgcgc ccgcgcccat caggcggcgc gtgttgtcgc cttcgagcgg
    11941 ctcgaccttg atcacttcgg cgccgaggtc cgcgagcacc agcccgcagc tcgggcccat
    12001 caccatgtgc gcgaactcta cgacgcgcac accctcaagc ggcagcacgg aatcctttag
    12061 gcaggccggc gagggccatg tggccgtaga gcggctcgtc gggcacgccg ccatgcatga
    12121 tcgcgcgcgc ggcaaggagc ttgtcgaaat cgatgccggt cttcacgccc atgctctcga
    12181 acatgaacac caggtcctcg gtgatcacgt tgccgctcgc gccgggcgcg aacgggcagc
    12241 cgcccaggcc ccccatcgac gagtcgaacg agcgcacgcc ttcctcgtaa gcggcgaggg
    12301 cattggcgag gccgaggccg cgcgtgttgt ggaaatgcgc gccctcgagc ttgtcgccga
    12361 tctcctgctt cacgcgcttg aacacgcgcc tgacttgcgc cgggttggcg tagccgacgg
    12421 tgtcggcaag cgcgacgcag tcgcagaact cggcgagctt cacggcaatg cgcagcacgt
    12481 cgtcctcggg cacgttgccc tgcatcgtgc agccgaaggc ggtggacacg gcgccttcga
    12541 tctccggcgt cttgccgctg cgcatcgctg ccgccttgcg cacttcgtcg accatctgct
    12601 gcggcgtcat gcgcacgttg gagacgctgt gctggtgcga ggcggagacg gggaaggtga
    12661 tcttgtgcgc cccggcctcg cgcgcgcgct cgaagccttt gacgttcggg gcgagggcga
    12721 cgaccttcag gcccttgagg gtcagcgcgt gcttcacgac ttcgccggtg tcggccatcg
    12781 cggggatgag cttcgggggc acgaacgagc cgacttcgat ctcgttcagg cccgccgcgg
    12841 caagggcgct gatccaggcc ttcttggtct cggtgggcat caactgcttg gcgttttgca
    12901 gcccgtcgcg cgggccgacc tcgcagatct ggacttggcg catggcgttc tccccggaga
    12961 ggcgaattct agcgcgcctc aatgcgcaga atcgcgtagc cgcagagggc gccacctgcg
    13021 gcgaacgcga gcatcgccca gagcgaggcg ccccagccgc cgaagctcga tgccagccat
    13081 gccagcagga tcggcccgaa gaactgtccc gcctgcgagg tctgcatgac catgccgttg
    13141 gtggtgccga tgtgctcggg cgtctttgcg tgcgccacga cgccggaaaa gatgcaggcg
    13201 ggaatgacgc cggcacacat ggagaacagc aggcaggacg cgtagcgcag cccatctggc
    13261 agcaggtcgc tcagcatgcc gatgtcggtc acgaccatca ccgcgcatgc gacgaggatc
    13321 aacgggccgc gccgcacgcc gtgcgccaga agccacccac ctcccagatt tcccggcacg
    13381 ttgatgagca ccatgaaggc ggtgagcagg gaggcgagtg cggcggaggc tccgcgttcc
    13441 tccacgagga acgtgggcag ccagatcatc accgaggtcc actgcgcgac gtagaacgcg
    13501 aagagcaggg cgagcgccac gcttgccggc tgcgacaggg actccagcgc gagctttagc
    13561 gagcccacgc cgccgtagcg cgtcgccggc gcgtgacgcg cggcgagcag gaacgccgcc
    13621 gcagccgcga ccgacacggc gacccacagc ccgcgccagc cccaggccgc gaggacgagc
    13681 ggcgcgagga ggattgcggc gctgcccccg gagggcatgt acgcgctcca cagccccatg
    13741 acgcgcgagc ggccgcgcgc gtcctccgcg ctcgccgcca tgagcgcgga gccggccacc
    13801 gcgtacagca tgaagccgac gccttcgaag aagcgcgacg cgagcaggga ggcgaagtcc
    13861 caggcggcgg cgccgaagag ccccgccagg cacatgacgg cgaggccggt gagcccaagg
    13921 cgcttgtggc cgaagcggtc gcacagcacg ccgaagagca tgccggcgag cccgccgatg
    13981 gcgttgaacg tggtggcgat gaacccggcc tggaccagcg acagctgcag cgcatcgcgc
    14041 tgctcgggca gcgccggggg caccttgccg acgtaggcgc cgcacaccag gccgccggcg
    14101 aacaccgccc acgccgccgg ccagcggatg ccggccatta cgcggcgtgc accgggatct
    14161 tcagctcgac gatcttcttg tgccagtcct gcggacccat ctggtggacc gacacgcctt
    14221 tcgcgtccac ggcgacggtc accggcatgt ccttgacctc gaactcgtag atcgcttcca
    14281 tgccgagatc cgcgaacgcc agcaccttcg acttgcggat cgccttcgcc accagatacg
    14341 cggcgccgcc gaccgcgatg agcgaggccg ccttgtgctt gcggatcgcc tcgatcgcca
    14401 ccggcccgcg ctcgcccttg ccgatcatgg cgaccaggcc ggtctgcgcc agcatcattt
    14461 ccacgaactt gtccatgcgc gtggcggtcg tcggcccggc ggggccgacg acttccttgc
    14521 cgaccggatc gaccggcccg acgtagtaga tgacccggtt ggtgaaatcg accgggagct
    14581 tctcgccttt ggccagcatg tcctgaatac gcttgtgcgc ggcgtcacgt cccgtgagca
    14641 gcttgccgga aaggagcagg gtctcgccgg gcttccacgc tccgacctcc gcgggcgtga
    14701 gcgcatcgag gtcgacgcgg cgcgcgtcct tcgagggcga ccaggtgacc ttcggccagt
    14761 catcgagcga cggtggcacg aactccgccg gccccgagcc gtccagcacg aaatgcgcgt
    14821 ggcgcgtcgc ggcgcagttc gggatcatcg caataggctt gttcgcggcg tgcgtgggat
    14881 aggcggcgac cttcacgtcc agcaccgtgg acaggccgcc caggccctgc gcgccgatgc
    14941 cgagcgcgtt caccttgtcg tagagctcga tcctgagctc ctcgtacttg tccttcggcc
    15001 cgcgcgcctt cagctcggcc atgtcgatcg gcgccatcag cgattccttg gccatgacca
    15061 tggccttttc ggcggtgccg ccgatgccga tgccgagcat gcccggcggg caccagcccg
    15121 cgcccatcgt cggcacggtc ttcagcaccc agtcgaccac cgagtcggac gggttcagca
    15181 tcgcgaactt ggccttcgct tcggagccgc cccccttcgc ggcgacgtcc acttcgacct
    15241 tgtcgccggg caccatctcg acgttgacca ccgccggcgt gttgtccctg gtattgcggc
    15301 gcgcgaactg gggatcctcg aggacggaag cgcgcagcgg gttgtcgggg tgcgtgtagc
    15361 cgcggcgcac gccttcgttc accgcgtcgg cgagcgcgcc cttgaatcct tcgaagcgca
    15421 cgtccatgcc caccttgaga aacacgttga cgatgcccgt gtcctggcag atcgggcggt
    15481 ggccctcggc gcacaggcgc gagttggtga ggatctgcgc gatcgcatcc ttcgcggccg
    15541 tcgattgctc gcgctcgtag gcgcggccga gggcccggat gtagtccgcc gggtggtagt
    15601 aggagatgaa ctgcagggcg tcggcgacgc tctgcaccag gtcttcttgc ttgatcgtcg
    15661 tcatgcgcga ccaccgtgac gtaaaggcgc ctgcggcatg agacggtcgc tcgccgcgat
    15721 cgccatcgcc gacagcagga acgtgatatg gatgccgacc tgcgcgatga tggtcttctc
    15781 gtcgtacgcc gaggcgttga tgaaggtctt cagcaggtgg atcgacgaga tgccgatgat
    15841 cgccgtcgcg agcttcacct tgagcaccga ggcgttcacg tgcgagagcc actcgggctg
    15901 gtcgggatgg ctctccaggc cgaggcgcga gacgaacgtc tcgtaaccgc cgacgatgac
    15961 catgatgagc aggttcgaga tcatcaccac gtcgatcagg ccgagcacca ccagcatgat
    16021 gacggtctcg gtgagcttgg ccgggcgcgc gacgccctcc accgtgaccg cgtcgatgac
    16081 gtggccgagg gcgttcgcgt tgccgaaagc ggcgagcagc aggtagtaca gctcctgcca
    16141 gaacagccac acgtacacgc cctgggcgac gatcaggccg aggtacagcg gcagctgcag
    16201 ccagcggctg gcgaaaatga gattgggcag cggcttcaac gggcgcaggg cgcgctcgcc
    16261 gggcaggggt gtcatcgggg cggcggaaga gttgcggaag gcgcttaatc tagcatacgc
    16321 ttgattcaat tcgacgcacg ttttcagagg aggagttgca tgtcgtccac catcgagtcg
    16381 gtgctgcagg agacccgggt tttcccgccc gcgcccgagt tcgtcaagca ggccaatgtt
    16441 ccgggaatgg aggcgtaccg gaagctgtgc gccgaagccg agaaggattt cgagggcttc
    16501 tggggccggc tggcgaagga acacctgctg tggcacaagc ccttcactca cgtgctcgac
    16561 gaatcgaagg cgccgttctt ccgctggttc cacgacggcg agctgaatgc gtcccacaac
    16621 tgcctcgacc ggcacctgaa gacccagccc gacaaggtgg cgatcatctt cgaggccgac
    16681 gacggcaagg tgacgagaat cacctacaag gcgctctacc acgaggtgtg caagctcgcg
    16741 aacgcgctca aggcgcacgg cgtaaagacc ggcgaccgca ttctcatcta catgccgatg
    16801 tcgatccagg tggtggtggc gatgcaggcg tgcgcgcgca tcggcgccac gcactcggtg
    16861 gtgttcggcg gcttttccgc caagtcgctg caggagcgca tcgtcgacgc aggggcgacc
    16921 gaggtcatca ccgccgacgg gcagttccgc ggcggcaagg agattccgct caagcccgtg
    16981 gtggacgagg ccttcgccat gggcggctgc gacaaggtga agaacgtgat cgtctacaag
    17041 cgcaccggca gcaaagtgcc gatgcaggcg ccgcgcgaca aatggtggga tgacgtggtg
    17101 aacggccagg ccgacacctg cgagccgacc tgggtgaatg cggagcaccc gctgttcatc
    17161 ctctacacct cgggctcgac cggcaagccc aagggcatcc agcactccac gggcggctac
    17221 ctgctgtggt gcgcgctcac catgaagtgg gtgttcgaca acaagcccga cgacgtcttc
    17281 tggtgcacgg cggacgtcgg ctgggtcacg ggccacagct acatcaccta cggtcccctc
    17341 gcggtcggga cgacggaggt catgttcgag ggcgtgccga cctatccgga cgccgggcgc
    17401 ttctggaaga tcatccagga ccacaaggtg aacgtgttct acacggcgcc cacggcgatc
    17461 cggtccctca tcaaggcggg gagcgacctg ccgaagaact acgacctgaa gtcgctgcgc
    17521 atcctcggga ccgtgggcga gccgatcaac ccggaagcct ggatgtggta ccacgaaacg
    17581 gtgggcggcg gacgctgccc gatcgtcgac acctggtggc agacggaaac cggcggccac
    17641 ctgattacgc cgctgccggg cgcaacgccc accaagccgg gctcggcgac cttcccgctg
    17701 ccgggcatca tggccgacat cgtcgacgag acgggcagga cggtgggccg cggcaagggc
    17761 ggcattctgg tgatcaagcg tccctggccc tcgatgttgc gcaccatctg gggcgatccg
    17821 gagcgctaca agaagactta ctacccggac gatttcaagg gcaagtatta ccttgcgggc
    17881 gacggcgcca gcatggacga cgagggctac taccgcatca tgggccgcat cgacgacgtg
    17941 ctcaacgtct ccgggcaccg tctcggcacc atggaggtcg aatcggcgct ggtggcgaac
    18001 tcgaagctgg tcgccgaggc ggcggtggtc gggcgcccgg acgagctgac gggagaggcg
    18061 atcgtcgcct tcgtcgtcac caaggggccg cgcccgaccg gcgacgaagc gaagcggatc
    18121 gccaaggagc tgcgcgactg ggtcggcaag gagatcggcc cgatcgccaa gcccaaggac
    18181 atccgcttcg gcgacaacct gccgaagacg cgctcgggca agatcatgcg gcggctgctg
    18241 cgcgccatcg ccaagggcga ggaaatcaag caggacgtct ccacgctgga gaaccctgcc
    18301 atcctggagc agctggcgca gaagcaatag cgacgcgcgc cgccctgcgg cggcgcaagg
    18361 gaggcggcat gggagagtac gatgtccggg gccagcgcct ggcggccttg tgcctgctcg
    18421 gcctgctgct gttcaactat ccgctgctcg ccgtgttcaa cgtgccgcgc atgatgttcg
    18481 gcattcccgt gctgtacgtg tacttctttc tcgcctgggc gtcgctcatc gggctgatgg
    18541 ccctggtgat cgagcggcgc ggttagcggc aagacaaggg ctcggcatgc tgcagggctg
    18601 ggtcgtcgtc cttacctcgt tcgcctacat cgggctgctg ttcgcgatcg cgtactacgc
    18661 cgaccggcgc gccgatgccg gccgctcgct gatcgacagc ccctacgtct acagcctgtc
    18721 gctcgccgtc tacgccaccg cgtggacctt ctacggcagt gtgggtcgcg cggccgaaaa
    18781 cggcgtggga ttcctcccca tctacatcgg cccgaccctg atgatcgcgc tgtggtggat
    18841 cgtgatgcgc aagatcctgc gcatcagcaa gcagaaccgc atcacctcgc tcgccgactt
    18901 cgtgtcctcg cgctacggca agagcgcgct gctcgccggc atggtgaccg tgatcgcggt
    18961 gatcggcatc gtgccctaca tctcgctgca gctgaaggcg atctcgaaca cctttgcggt
    19021 cgtgctgcag tacccggaca tcgtcatgcc ggccaagctg ggcaccgtgc cggtgctgca
    19081 ggacacggcc ctcttcgtgg cgctgttcct cgccgcgttc acgatcctgt tcggcacgcg
    19141 ccatctcgac acctcggagc atcacgaggg catggtggcg gcgatcgcct tcgagtcgct
    19201 ggtcaagctg gtggcgttcc tcgcggtcgg cgcctgggtg acctggggca tctacaacgg
    19261 cttcggcgac ctgttcgcgc gggctgccgc ggagccgaag ctcgccgcca tgctcacgcc
    19321 cttcgacgcc gtctccggca gctactcgag ctgggtctgg ctcaccatcc tgtcgatgat
    19381 ggcgatcatg ttcctgccgc ggcagttcca ggtcacggtg atcgagaaca aggacgaaaa
    19441 gcacctgaac aaggcgatct ggctgttccc gctgtacatg ctggcgatca atgtgttcgt
    19501 gctgccgatc gcgttcggcg ggctgctgca cttcccgggg ggcggcgtcg acgccgacac
    19561 gttcgtgctg acgctgccga tggtcgagaa gcaggaactg ctggcgctga tcgtgttcat
    19621 cggcgggctg tccgcggcca ccggcatggt catcgtcgag accatcgcgc tctcgaccat
    19681 ggtgtgcaac gacctggtga tgccggtgct gctgcgcatg cgcgcactgc gcctgaacga
    19741 gcggccggac ctgagcggat tgctgctcgg catccggcgc ggcgccattg tggcggtgct
    19801 gcttctcggc tacctgtatt tcagggtggc gggcgaggcc tacgcgctgg tcgccatcgg
    19861 cctgatctct ttcgcggcgg tggcgcagtt tgcgccggcg gcgctgggcg ggatcttctg
    19921 gaagggcggc acgcgcctgg gcgcgctcgc ggggctggcg gcgggattca ccgtgtggtt
    19981 ctacacgctg ctgctgcccg ccttcgcgcg ctccgggtgg ctgccgatcg ggttcctgga
    20041 agaaggacct tggggcatcg cgctgctgcg gccgctcgag ctgttcggcc tgggcggact
    20101 ggatcagatc acgcacgcga tgatctggag catggcggcc aacatcggcg cctacgtcgg
    20161 ggtgtccctg ctgcgcgcgc ccggcccgga ggagctgcgc caggcgagca tgttcgtgga
    20221 cgtgttcacg cggcccgcgc cggtgggcgg ggcgggattc tggcgcggca aggcccccgc
    20281 cgccgagctg cagaacctgc tggcgcgctt cgtcggcgca ggcgccgcgc aggaggaatt
    20341 cgccgagtac gcgcgcacgc gtggcctgga gccgtcggcg ctgggcccgg aggcggacgc
    20401 cgacctcgtc tatttcgccg agacgcatct tgccggtgtc atcggcgcgg catccgcgca
    20461 catcatggtg gcctcggtgg tcaaggaaga ggcgctgacg ctcgacgaag tgcgcgcgat
    20521 cctcgacgaa gcgtcgcagg tggtggtgta tagccgacag ctggagcaga agtcgcgcga
    20581 gctcgaggcc gcgaccgccg agctgcgcag ggcgaacgag cggctgcagg agctcgatcg
    20641 gatgaaggac gatttcattt ccaccgtcac gcacgagctg cgcacgccac tcacctcgat
    20701 ccgcgcgttc tccgagatcc tgcgcgacga ccccaagttg gcgctgccgc agcgcaccaa
    20761 gttcctcgat atcatcgcca aggaaaccga gcggctcacg cgtctcatca accaggtgct
    20821 cgatctcgcg aagctcgagt cgggcgcggc ggagtggcat accaagtcgg tcgacatgcg
    20881 cgagatcgtg gctgagaccg tcaccggcat gagcgaggtc ttccgggagc gcgcggtgcg
    20941 cctggagttg cgcatgccgg gcatcgtccc gccggtgcac gccgatcccg accgcgtgaa
    21001 gcaggtgctg ctcaatttgc tgtcgaacgc cgccaagttc tgccaggccg accgcgggcg
    21061 tgtcgaagtg tccctgctcg aggaaaggga cgcggtgcgg gtcgacgtga aggacaacgg
    21121 gcccggcatc agcctggcgg accaggagat catcttcgag aaattccgcc aggtcggaaa
    21181 cgcgctgacg gacaagccgc agggtacggg cctcggtctg cacatctgcc gccagatcgt
    21241 ggagcgactc ggcgggcggt tgtgggtaga gagccgggtg ggtgagggcg cgtgcttctc
    21301 gtttacgctg cccgtgccgc ggcaccgcca caccctgagc gcgcgcaccg catgagcgac
    21361 gccgcgcccg gaggccgcac tgaggagggc ctggagtgag caagaaagtg ttgcttgtcg
    21421 acgacgagcc gaacatcgtg gccgcgctcg aattcctgct cgagaagaac ggctacgatg
    21481 tgaaggtggc cgccaatggc gtggaggcga tcgcgcagct cgatgcgttt cggcccgacc
    21541 tcgtgctgct cgacgtgatg gtgccgaagc tgagcggcta cgaggtatgc cagcgcatgc
    21601 gggcggagcc caagtggcgg gacatcaaga tcgtgatgct ttcggcgaag ggccgcgagg
    21661 tggaggtaaa caagggcatg tcgctggggg cggatctgta cgtgaccaag cctttctcga
    21721 gcgcggagct ggtggccact atccaacagc tgctcgagca tcgcgcgtga aggcccgcgg
    21781 caagttcgcg ctctgggtgg cggttttgtt tgccgcgggg gtgggggtgc cgctggccac
    21841 cgcactagcg gtggtggcgg cactggcggc gcccgaccgc gacgtcgtgc tgggcatact
    21901 ggagccgcgc atgccgctga tcatctttgg cgctctggcg ctgttcgcgc tatgcgcggg
    21961 cactgtccga tggctgtttg cgtcgtacgt agagccggcc gcggcgctcg ccgaactgac
    22021 gcgcggcgcg cgggccgccc aaggggacac gccggtggcg agtgacggcg cgggcgagct
    22081 ggtgcggctc gcccgcgaga tcgaccagct ggcgcaggaa acgcggcgcc tgcgcgccgt
    22141 gtcggacgcg cgtgtgcagg cggcgctcgc ggatctggag gaagagcgca accggctcgc
    22201 ggcgctgatg tcggagctcg tcgaaggcgt gctcgtctgc aacgccgagg gccgcatcat
    22261 gctctacaac gagcaggcgc gggcgctgct gcgcaacgac gcgagcccgg cggcagcggc
    22321 gccggtcggg ctggggcgat cgatcttcac gctgatcgat cgcgaacagg tggcgcacgc
    22381 gatcgagaag ctcgagcagt cgcgccagcg cggcgcagcc gcgccgcgca cgcgcttcgt
    22441 ggcgcccgcg ggcccggcgc gcctggtcaa gttcgaggcg gcaccctatc gcggcgcggg
    22501 cggcgagctg gccgggatcg tctttaccct gagcgacgtg accgggttgc tggagcgcga
    22561 ggcgcagcac cttgcgacgc tgcagtcgat tgccacccac gcccgtgccc cggtggccaa
    22621 tatccgggca gccgcggaga acctggcgcg ctaccccgac atggaggcgg aacggcagcg
    22681 ccggttcgtc gacatcgtcg tcgacgagtc gcagacgctg tcgcactcga tccggcgtgc
    22741 gctgcagacc tacggcgagg cgttgaaggc gagcctcatc ctggaggaca tgcaggcgct
    22801 ggacctgctg cgcgtcgccg aggggcgcat acgggcgctg gcgggggtgg aggcggcact
    22861 cgagccgatc gacgaatcgc tgtggctgac ggtggacagt ttcagcttcg tgcacgcgct
    22921 gtgctttctt gccgcgcgcc tcaaggagga ctacggggtg caccggatcc gcttcgccgc
    22981 gcgggccgcc gagcggttcg tcgagcttga tctgggctgg cgcggcgccg tggtcggcca
    23041 cgacgcgctg acacaatggg aaagcgagcc gctgcgcgct ggcgcgcagg aatcgccgct
    23101 cacgctgcgc gacgtcatcg agcgtcacgg cggcgagctg tggctgcagc gcgaggggcc
    23161 ggccgggcag cgcgacgcct gctttcgctt cctgctgccg gccggcgagc ccgtcgccgc
    23221 cccggtgcgg gcggaagcct cgatccagag ccggcccgag ttctacgatt tcgacctgtt
    23281 cgcgcacgcc ggcgcgtcgc tggacctgag ggagcggccg ctcgccgagc tcgcttatac
    23341 cgtgttcgac ctcgaaacca ccgggctcgc gccctcgggc ggagacgaga tcatcagcat
    23401 cggagcgatt cgcgtcgtca atggacggct gctgcgaagc gaggtgttcg agaagttggt
    23461 cgacccgcgg cgcccgctga acccggaatc cgccaaggtg cacggtatcg acgcgcgcat
    23521 gctggagggc cagcccaaga tcgaggaggt cctgcccgcc tttcaccgct tttgcgagga
    23581 cacgatcctg gtggcgcaca acgctgcgtt cgacatgcgc tttctcgaat tgaaggaaaa
    23641 ggcggcgcgc gtgcggttcg agcagccggt gctcgacacg ctgctgctgt cggcggtggc
    23701 gcagcccgcg cagcaggacc atcggctcga ggcgatcgcc gcgcggctcg gcctgccggt
    23761 catcgggcgg cacactgcgc tcggtgacgc gctgctcacg ggcgaagcat tcctgcgcct
    23821 gcttacgctc ctgcgcgagc agggaatcgc gaccctgggg caggcgctcg acgcatcgcg
    23881 tgagacttac tatgcgcggc tgcgctattg agcccggggg ccgccggcat ggctgagccg
    23941 cgccccgatc tgcgcgagcg catggcggga gagcgccagg cgttcgccct gcccctgcag
    24001 gctctcgtgc ggcgcgcggt gatcacctgc gaggagcagc gcagcgtcgc cgaggccgcc
    24061 cgcatcatgc aggacagcga cgtgggctcg gtgatcatcg tcgacgccca ctcgcgcccc
    24121 gcgggcatct tcaccagccg cgatctggtg caggtggcga gcgcgggcgg cctggagcgg
    24181 cgcgtcacgg atttcatgag ccgcgacctg gtcacgctgc ccccgcacgc cttcgcctac
    24241 gaggccgcgc tggagatgac cgagcggcgc atccggcaca tcctggtgac cgagggcggg
    24301 cggctgctcg gggtggtgtc ggagcgcgac ctgttcgcgc tgcaacggct tgggctgggc
    24361 gaactcacca tggaaatccg gctcgcggac agcatcgaga cgctggcggg tctcgcggag
    24421 caagtccgcg gcctcgccac gctgctcatc gaacagggca cggcgcccga gccgctgacg
    24481 ctcttcatct ccgtgctgaa cgaccgactc acgcgtcgcg tcatcgaggt ggtgcgccgg
    24541 cgccacgagc tgaaccgcat ccgctggacc tggctagcct tcggcagcga aggccgcctg
    24601 gagcagacgt tcagcacgga ccaggacaac gggctggtgt tcgcagcacc ggagggcgtc
    24661 gcgcccgacg cggcgcgcgc gcaactgatc cccttcgccc acgaggtcaa tcgggcactc
    24721 gacgcctgcg gattcccgct gtgcgagggc aacatcatgg cgagcaaccc cgcgctgtgc
    24781 ctgacgctgg aagaatggcg agcgaaaatg accggctggc tcaacgtcac gagcccgcaa
    24841 gcgctgctgg acgccgcaat ctgcctcgat ctgcggccga ttcacggcga cgaggtcctg
    24901 gctacggagc tgcgcggctg gatcgtcgag cgggtgcgcg ggcacgcccc cttcctgagg
    24961 ctgctggcgc aggtggcgac acggatgcgg ccggcgcgca gccgcttcgg cgcgctcgtc
    25021 acgacggacg cgcccggggc gccgcgcacg gtgaacctga aggcgaacgg cgcgcgcatc
    25081 ttcgtcgacg cagcgcgcgt gctcgcgctg gcgagcggtg tacgccagac caacacggcc
    25141 gaccgccttc gcgcctaccg cgaggtgcgc gatcagcccg aggccgagac gggcgccctg
    25201 gtcgatgcgt ttcatttcat ccagggcctg cgcctgcgca tccaggcggg cgcgcgcaag
    25261 gcgcgcgcgg gcgggcgtgg tgccggcgag gacctcgcca atcgcattga cccgagcgcg
    25321 ctcaacgcct tcgagcgatc ctgcctcaag gaggcactcc ggctcgcgcg cgagctgcag
    25381 cgtcgactcg agctcgactt cctggtatga ggcacggacc ggacgacgag cgcgcagccc
    25441 agcagacgcg gccgacggcc tacatcgtcg acgacgacga gtcgatccgc acgctttggc
    25501 gctggcttct cgaatcgaac ggcatggcgg tcgaggcgtt cgcgacggcc aaggagttca
    25561 tcggcgcgta ccaaccgggc gggccggcgt gcctcgtgct cgacctgcga ctgcccggga
    25621 tgagcggcct ggagctgcag gagtatctgc gggagcgcga aattgcgatc cccatcgtct
    25681 tcgtcaccgg gcacggagac gtgccgactg cagtgagcgc catcaagggc ggcgcggtgg
    25741 acttcatcca gaagcccttc ggctaccgcg aggtcctggc catcatccag cgcgcgtttg
    25801 agcgtgacgc cgaggtccgc gagcagcgca cgcggcgctc gcaggccgcc gagcgcctcg
    25861 cgacgctgac cgaacgcgaa cgcgaggtca tgcagcgtgt ggtcgagggc aagttcaaca
    25921 aggtcatcgc cgacgagctc ggcatcagcg tcaagacggt ggagttccac cgcgccaagg
    25981 tgatggagaa gatgggcgcg gactcggtgg cggggctggt gcagctgatg atgcagcacg
    26041 caagctagtc ttcgcaccat gtgctgcgcc gcagcagttt gctgcggcgc agcggccaac
    26101 cttaagggtt tcccccaatg gttgcgggca tgtgccgcgg atagcatcca acgtcctaac
    26161 tactgcgcgg cggatgaaag taccgccacg ggatccagat tgtggctgag atcgcgttgc
    26221 acctcatgcc agcggaagcg caccgagttg cgcacgatgt ccgcatccag tgccttgagg
    26281 tcgtcgacga tcggcgccca gtagacaagg gcgtccgggc tttcggcgaa cgcgacttca
    26341 ccctccgttg cattccatgc acaaagcgcc agccgcgcgc gcacgcgctg cgcatgtgcg
    26401 gcgaactccg gtccggcctc ggcgggggtg cccgtttcca gcagcagttg gtcgaccagg
    26461 cgctgcatca ccgagccgat ctcgtcaggc gaggcaccgc tggcgcagcg cagcattcca
    26521 ccgacgtgca ccgcgagatc cgacaggcag gcgagccaca cgtgccattt ggcgatgttc
    26581 atggacgcca cgaaagcgtc gttctcgaac agttccggaa agcgggtgcc ggcgcgcgtg
    26641 cgcaggtagc cgtagagcgt catctgcgcg acatgactgg cgcgcgactg caggaaggcg
    26701 cgcagtgcgc cgacatcccg gatcggctct ctgcggcgcc agcggcggta ccagcggcgc
    26761 agcgcctcca ggcggcgggc gaggaatggg tgcaccgccg cattatccag cgtccggcct
    26821 tgcgcggcca tgattgacat gagctagaat cggccggcct gattgatcgg ggcccgggga
    26881 gggacgccgg gcgaggggac aacaagagag gagggcatgc tatggccacg gaagaggcaa
    26941 acagggtagt ccactggcag cggaccagaa ccttgatgat ctggcacatg atcatctggt
    27001 tcatcttttc cttcctggtg cactggttcg cgacaaatct gaacgccata accttcatgc
    27061 aattcccgct gggcttctac atggcggcgc agggctcgct cgcggtcttc gtcgtgcagc
    27121 tgttcgtgtt tacgtggcag cagcacaaga tcgacgtcga gtgcggcatg gccgaaaccg
    27181 aataagggga gaccagaaaa tggctatgtt tggggatttc tcgagcaagg gcgggttcat
    27241 tgagaacctg cccaagatct acggcttgta caccggcggc ttcgtcgtgt tcgtcggcct
    27301 gatggcgatc gccgagcaga tgggcgtcaa ggaagacacc atcggcctga tgttcgtggg
    27361 cttcacgatc gccatctacg cgctgatcgg ctggctctcg cgcaccatgc agctcgacgc
    27421 gtattacgtt gcgggacgcc aggtgccgac cgtgttcaac ggcatggcga ctgcggcgga
    27481 ctggatgtcg ggcgcgtcgt tcgtggccat ggcgggcggc atctacctgg gcggtcaccc
    27541 gtacctcgcg ttcgtggtgg gctggaccgg cggctatgtg ctggtggcgg tgctgatggc
    27601 gccctacctg cgcaagttcg gctgctacac ggtgcccgac ttcatcggca cgcgctatgg
    27661 cggtaatttc gcacgcctgt gtgcggtgct ggtgctggtg gtcgcgtcgt tcacctacgt
    27721 gacggcgcag atcaatgcca ctggcaccat cgcctcgcgc gccctgctga tcccgttcga
    27781 ggtcggcatc tggatcggcc tcgcgggcat tctgctgtgc tcgatgctcg gcggcatgcg
    27841 cgcggtgacc tggacgcagg tggcgcagta catcgtgctg atcatcgcct accttgtgcc
    27901 ggtcatctgg atgtcgaaca agcagggctt cggcgtgatt ccgcagttcg tgtacggcga
    27961 tgcggtgcag cgcatcgcag agctcgagca gatgcaccag gtcggggtgc tcaaaccgag
    28021 tgagacggtg gccggcttga gcgcgttggt gaccaagcac aacgcgccgg cggactcctt
    28081 cgtcgccgcg tggcggtttc tgacgctgac ggctgccatg atgatcggca cggcctcgct
    28141 gccgcacatc ctgatgcgct acttcacgac gccctcggtc aaagcagcgc gcgcctcagt
    28201 ggcttggtcg ctgttcttca tcttcctgct gtacttcacc gcgcccgcgc tggcgacgtt
    28261 caccaagctg cagatgctcg atccgaatct ggcgacaagc gtcatcggca agtcgctcgc
    28321 agaagtgaac gcgcacgtct gggtgcagaa gtgggcggcc gtaggcggac tgaaggtgat
    28381 cgacagcaac ggcgacggca tcgtgcaggt caacgagttc ttcattcgcg gcgacattgt
    28441 cgtgctggca acacccgaga ttgcgggctt gccatacgtc atatcaggac tggtcgccgc
    28501 aggcggcatg gcggctgcga tgtccactgc cgacggcctg ctgctcgcga tcgccaatgc
    28561 gctctcgcac gacctgtact acaagatgat cgacccgaag gcagacgtga aacgccgcct
    28621 gatcgtggcg cgcgtcctgc tggtcgtgat cggcgccgcg ggggccttgg tggcgagcct
    28681 acgcctcacc gggatactgg gcgcggtcgc ttgggccttc tgcttcgcga tgtctggcct
    28741 gttcttcccg ctggtgctcg gcatctggtg gaaacgcgcc acatcggcgg gcgccatcgc
    28801 cggcatgctc ggcgggctgg cggcgggttc gtggtacctg tacatggtct atgccggcgg
    28861 catggcgccg tggtggggca tggatcacct gcgcttcggc atagttggtg cagccgtgag
    28921 cctcgtgctg atggtcgtgg tgtccctgct cacgccggcg cccgaccagg cgacgcagga
    28981 catggtcgac gaagtgcgca tcccgaaggg tcagacgatc ctcggcacta cgcactagcg
    29041 cagtcagcag cagacaaacc ggggcgaaga gtcttcgccc cggttttttt gcgcccgtaa
    29101 ggctctacaa tttccggctg gcatcggact ccatggattt cccctttccc ctttgggtca
    29161 tcatcctcga ttacttgctg ggggtcgtga tgtggacgct catcgggcgt accgccatga
    29221 ggatcttcct ccctgagaac agcagcttct ttttcatgcg gttcttcgtg cgctcgaccg
    29281 acccgctgct gaagctgttc cgaccgatca cgccggcgtt cctcattcag ccgctcgtgc
    29341 ccctgtacgt ggcgtggttc ttctttctcg tccgcttcta cgtgatgccc ttgctgctcg
    29401 gctacacggt aatgggcatg ctctcctttc cgcttgaagg ggagatcgcg cggagcattt
    29461 cccgattgat gcagaagatc ggctattagg cccggagccg ccggcgcatg ctcgcttgag
    29521 gccgggcgga tggaccgcga ggtagaatag ccgcgtcacg gagagatggc cgagcggctg
    29581 aaggcgcacg cttggaaagc gtgtatacgt taatagcgta tcgtgggttc gaatcccact
    29641 ctctccgcca gtaagcgatc aacggcgggc ctccccgcat tgcagtccgg tgaacctggt
    29701 caggtccgga aggaagcagc cacagctgga tcctgcaagt gccgggggta gggctcgcca
    29761 cccatgccgc gcgaaagggg actgcggtcc cccttcgacc cccgctgata aaattgacgc
    29821 catgtcctat caggtcctcg cgcgcaagtg gcggccgcgc aatttcgcgt cgctggtggg
    29881 gcaggagcac gtggtgcgcg cactgcgcca cgcgctggaa cagaagcggt tgcatcacgc
    29941 gtacctgttc accggcacgc gcggcgtggg caagaccacg ctggcacgga ttctcgccaa
    30001 gtgcctcaat tgcgaaaccg gcatcacggc ggagccctgc ggacgctgca gcgcctgcga
    30061 ggagatcgac ggcgggcgct tcgtcgacct gctggaggtg gacgcggcta ccaacacgcg
    30121 tgtcgatgag atgcgccagc tgctggatac ggcgcagtac gcgccgacgc gcggccgctt
    30181 caaggtgtat gtcatcgacg aagtgcacat gctgtccaac tcggcgttca acgcgatgct
    30241 caagacgctc gaggaaccgc cggagcatct caaattcgtc ctcgccacca ccgatccgca
    30301 gaagattccg gtcaccgtgc tgtcgcggtg cctgcagttc aacctcaagc agatgccgcc
    30361 cgcggccatc gccggacatg tggccgagct gctgaaggcc gagagcatcg cctttgagcc
    30421 cgacgcgctg caattgatcg gccgtgccgc gtccggcagc atgcgcgacg cgctgtcgtt
    30481 gctcgatcag gcgatcgccc acggcggcgg ccgcgtggcc actgagggcg tgcgcgacat
    30541 gctgggcgcc gtggatgaca cctacctgtt gcgcctgctt ggcgcggttg cgaccagcga
    30601 cggtgcgcag ctcatggcca tcgccgatga gatgcaggcg cgaagcctgt cgttcgactc
    30661 tgcgctgcag gatctcgccg gcctgctgct cgagctggcg gtgatgcaag ctgccgcgga
    30721 tgccgtggat gcggaccggc ccgggtacga ggcgcgcgcg gcgctggcgc aacaattaga
    30781 cgccgaaacg gtgcagctgt gctatcagat tgcgctccaa gggcgtgacg accttccgct
    30841 tgcgcccgac gagcatgcgg gattcgtgat gacgctgctg cgcatgctcg cgttccggcc
    30901 ggaggcggcg gctgcggagg aacgccccag cggcgctgcc gccacagcgg caccggccgc
    30961 agcccgcgcg gcacaacgca aggagttcga cggcgattgg ccggcgctcg tgcgccggct
    31021 ccccgtgggc ggggcggccg gcgagctggc gcgcaattcg gtgctgcggc gccattcggc
    31081 cggggcgatc gagctgatcg tccccaaggc catggcgcac ctcgccgagc gcggctacca
    31141 ggagaagttg aaggcggtgc tggagcagca tttcggcgtc ccgatggcgc tgaaggtggt
    31201 cgcgggcgag gcaccgggcg agaccgcggc gagccgcgag gccggagagc gcgaggcacg
    31261 tcgcgccgaa gcagcgcgcg ccgtccaggg cgaccggttt gtccaggacc tcgtcgacat
    31321 cttcgatgcc aaggtcgtgg gatcgagcat caaggccaac ggcaacaagg actaggggga
    31381 ggcgagagga tgcgcggaaa catcggccag ctgatgaagc aggcccagat gatgcaggag
    31441 aacatgcgcc gcatgcagga gcagctggcc gccatggaag tggaaggtca ggcggcctcg
    31501 ggcaaagtga aagtcgtgat gacctgcaag cacgaggtgc ggcgggtgag catcgacccg
    31561 gaactcgccg cggaggaccg cgagctgctc gaggacctca tgatcgcggc cttcaacgac
    31621 gccgcgcggc aggtcgacgc gctggtttca gagaagatgg gcggcatgac tgccggcctc
    31681 gggttgcccg ccggcttcaa gctgccgttc taaggccgtg agccgggacg cgatcccaag
    31741 cgcgaagggc ctcgagcggc tggtcgaggc attgcgcgtc cttcccggcg tgggcccgcg
    31801 ctccgcgcag cgcatggcgt attacctgtt gcagcgcgat cgcggcggtg cggagcagct
    31861 cgcgcacgcg ctgtccgagg cgctcgcccg ggtccggcat tgcgcgctct gcaacaatta
    31921 caccgaggat gaggtgtgcg cgctatgccg ctcgccgcgg cgcgaccgta cccagctctg
    31981 cgtcgtcgaa acaccggctg acctcgccat ggtggagcag acctccgcat acgctggcat
    32041 gtactttgtg ctgatggggc ggctgtcgcc ccttgacggt gtcggcccac gcgatatcgg
    32101 cctggaaaag ctgctcgcgc gcgccacgga aggcgatgtc gccgaggttg tcctcgccac
    32161 caacttcacc aacgagggcg aagcgaccgc gcactacgtc gcggagctcc tgcgcaaccg
    32221 gaacgtgcgc gtgagccgca tcgccagggg cgtcccgctg ggcggcgagc tcgagtacgt
    32281 ggacgccggg acgctgtccc aggcgctgct ggaccggcgc ggaatgtagg ccggtcgtcc
    32341 gcggtgccgg ccgctgcatt ttgttgacac tatttttgcg ggggcgctat aattccgcct
    32401 cttcggacgc ggggtggagc agtctggcag ctcgtcgggc tcataacccg aaggtcagag
    32461 gttcaaatcc tctccccgca accagctctt taaaaattcg cagccgataa gtgtgggcgc
    32521 tcgatgcaga aggccaaggc atctagtgct cgcaataggt aatacgaagt aactccgttt
    32581 tactttgagc gagaagttgg cgcggccgca agccgcgctg gcgaccggca gcaatgccgg
    32641 actgtttcaa gctttttaat tgaagagttt gatcctggct cagattgaac gctggcggca
    32701 tgccttacac atgcaagtcg aacggcagca cgggggcaac cctggtggcg agtggcgaac
    32761 gggtgagtaa tgcatcggaa cgtgtcctct tgtgggggat aaccagtcga aagattggct
    32821 aataccgcat gagatcgaaa gatgaaagcg ggggaccgca aggcctcgcg cgagaggagc
    32881 ggccgatgcc ggattagcta gttggtgggg taaaggccca ccaaggcgac gatccgtagc
    32941 tggtctgaga ggacgaccag ccacactggg actgagacac ggcccagact cctacgggag
    33001 gcagcagtgg ggaattttgg acaatgggcg caagcctgat ccagccatgc cgcgtgtgtg
    33061 aagaaggcct tcgggttgta aagcactttc ggacggaacg aaatcgcgcg catgaacaat
    33121 gcgcgtggat gacggtaccg taagaagaag caccggctaa ctacgtgcca gcagccgcgg
    33181 taatacgtag ggtgcaagcg ttaatcggaa ttactgggcg taaagggtgc gcaggcggcc
    33241 ccgcatgtca ggcgtgaaat ccccgggctc aacctgggaa tggcgtctga aactacgggg
    33301 ctggagtgtg gcagagggag gtggaattcc acgtgtagcg gtgaaatgcg tagatatgtg
    33361 gaggaacacc gatggcgaag gcagcctcct gggccaacac tgacgctcat gcacgaaagc
    33421 gtggggagca aacaggatta gataccctgg tagtccacgc cctaaacgat gacgactagt
    33481 tgttggggga gttaaatccc ttagtaacgc agctaacgcg tgaagtcgtc cgcctgggga
    33541 gtacggtcgc aagattaaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggatga
    33601 tgtggtttaa ttcgatgcaa cgcgaaaaac cttacctacc cttgacatgt caggaatcct
    33661 ccagaaacgg gggagtgccc gaaagggagc ctgaacacag gtgctgcatg gctgtcgtca
    33721 gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg ccattagttg
    33781 ctacattcag ttgagcactc taatgggact gccggt
//
LOCUS       DBNHKE010000191         5969 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_192040, whole genome shotgun sequence.
ACCESSION   DBNHKE010000191 DBNHKE010000000
VERSION     DBNHKE010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_192040"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC16O9_13075"
     CDS             <1..537
                     /locus_tag="AC16O9_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR6517584.1"
                     /translation="APDTPRLQGEDGFSRKGPRPQEASYYYSRPQLKAHGTLDGDKVS
                     GVAWLDHEWSSQYLAPEAAGWDWCGLNLDDGSALMAFRIRDQQGGVYYAPDGISFTPL
                     RRWRSPRTGVEYPVAMRVAAGGEQYDLTPLMDDQELDARSSTGTIYWEGAVRALRAGS
                     EVGRGYLELTGYWRPMTL"
     gene            complement(534..1160)
                     /locus_tag="AC16O9_13080"
     CDS             complement(534..1160)
                     /locus_tag="AC16O9_13080"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6517585.1"
                     /translation="MKPLAFDATLRARLATNLSRHAPAPSTPHGLKRAAVCVLVTDDG
                     RGEAALVLTRRAEHLSTHSGQYALPGGRVDGGESTLAAARREAREEIGLELAPQAFLG
                     ALDDYATRSGYLITPLVAWVADGARLAPNPAEVAKIYRVPLAELGRHGSPQFVSIPES
                     DRPVIRYPLLGTLIHAPTAAVLYQFMEVAVLGRATRVAHLEQPVWAWR"
     gene            1219..1986
                     /locus_tag="AC16O9_13085"
     CDS             1219..1986
                     /locus_tag="AC16O9_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517586.1"
                     /translation="MPYVTCAGHALEYEWVGAAAPGAPTLVFLHEGLGSIRQWRDFPQ
                     QVVDATGSRALVYSRYGYGRSDVLAEPRVGVDFMHREALDALPELVAQLGLERPILVG
                     HSDGASIALIYAGAGHPVRAVAAMAPHVFVEPICVDSIRAARAAFGATDLGQRLGKYH
                     RDPRKTFHLWADAWLDPEFLKWSIEEMLPQIACPVLAIQGEDDEYGTLEQLERIRRGV
                     RGTCEIVKLPACGHAPFRDQPQAVLDALTRFVHEHGR"
     gene            1976..3172
                     /locus_tag="AC16O9_13090"
     CDS             1976..3172
                     /locus_tag="AC16O9_13090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517587.1"
                     /translation="MAADVLRQDVKVIGLVALAHGLSHFFQIATAVLFPLIKDDLGVS
                     YTALGATVALYFTVSGICQTLAGFAVDRFGARRVLFFGLVLAVAGALVAGLAQNYSML
                     VAAAVVGGLGNSVFHPVDFSILNARVTVRRLGYAFSWHGIAGYLGYASAPAYGIAMAS
                     AFGWRGALLGAAVIGAAVVLLLTLQRGSLHVEPADARRAPAGLGADLRVLAQLPVVMV
                     FGYFVMLGVAFVGIQTFGIAAMLSLYGVNVALASAALTAYLVGGAAGIFCGGFVAVRA
                     TRHDLVAVSGMAASAALFSVLAMGWLPGTLLPLLLGAAGFTSGLTNPSRDLIVRGATP
                     AGATGKVYGFVYSGLDVGSMVTPVIFGWLLDRGEPAVVFYAVVGVLVLSILTVLRLPE
                     RAPQPA"
     gene            complement(3160..3942)
                     /locus_tag="AC16O9_13095"
     CDS             complement(3160..3942)
                     /locus_tag="AC16O9_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR6517588.1"
                     /translation="MSAVLKPKSSKPVRERVSREEWQMRVDLAAAYQLAALFKWTDLI
                     YTHFSARVPGSEDFLINAYGLMFDEITASNLVRIDARGKVLDDPLELGYNEAGFVIHG
                     CMHEARPEINCVIHTHTRAGVAVSAMKCGLLPISQHAMRVQQQCTYHDYEGIALNLSE
                     RERMAASLGQRSKVMILRNHGLLTLGETVREAFELMYYLDCACQIQVDAMAAGLENVQ
                     LMSQSAADTATEQFQRDDRPSTHKDWPALLRLLERRGVDYAG"
     gene            complement(3899..4882)
                     /gene="dusA"
                     /locus_tag="AC16O9_13100"
     CDS             complement(3899..4882)
                     /gene="dusA"
                     /locus_tag="AC16O9_13100"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIR6517589.1"
                     /translation="MSAHRLCVAPMMDWTDRHCRYFLRQVSARAFLYTEMITTGALLH
                     GDVERHLGFNAEEHPVAAQLGGSEPEDLARCAKLVQEYGYDEVNLNCGCPSERVQKGA
                     FGACLMAEPGLVAECVSAMRESVSIPVTIKHRTGIDRIEAYEFMARFVDIVSRAGAAT
                     FIVHARNAVLKGLSPKENREVPPLKHDYVYRLKQDFPQLEIVINGGITGWQQADAHLA
                     RVDGVMLGRAAYHDSWLLADPGKTRRQVVRAMAEYAAGAPGLRHVARHMLGLYHGHPR
                     ARLWRRMLSDSARLSQNDPALLLEALAAVESASKETPHERRPQAKELQARP"
     gene            4919..5857
                     /locus_tag="AC16O9_13105"
     CDS             4919..5857
                     /locus_tag="AC16O9_13105"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR6517590.1"
                     /translation="MHKILVTGGAGFLGSHLCERLLGAGHDVICLDNFYTGGKANIKH
                     LLGRPEFELLRHDVTFPLYVEVEQIYNLACPASPIHYQRDPVQTTKTSVHGAINMLGL
                     AKRTRARILQASTSEVYGDPEVHPQAEGYWGRVNPIGPRSCYDEGKRCAETLFFDYYR
                     QHQLDIKVVRIFNTYGPRMHPNDGRVVSNFIVQALRGEDLTIYGKGTQTRSFCYVDDL
                     IDAMVRMMGTARGFTGPVNIGNPGEFTMLALAEMVLRLVGGKSKLKYLPAPEDDPMQR
                     QPDIALARDKLGWAPKVALEDGLKETIAYFRKTLAA"
BASE COUNT          875 a         2015 c         2115 g          964 t
ORIGIN      
        1 gcgccggata cgccgcggct gcagggcgag gacggattca gccgcaaggg accgcggccg
       61 caggaggcaa gctattacta cagccggccg caactgaagg cgcacggcac gctcgacggc
      121 gacaaggtga gcggcgtggc gtggctggac cacgagtggt ccagccagta cctcgcgccc
      181 gaagcggcgg gctgggactg gtgcggactg aacctcgacg acggcagcgc gctgatggcg
      241 tttcgcatcc gcgaccagca gggcggggtg tactacgccc ccgacgggat ctcgtttacg
      301 ccgctgcgcc gctggcgctc gccgcgcacc ggcgtcgaat acccggtggc gatgcgcgtc
      361 gcggcgggcg gcgagcagta cgacctcacg ccgctgatgg acgaccagga gctcgacgcg
      421 cggagcagca caggcaccat ctactgggag ggcgcggtgc gcgcgctgcg cgcaggcagc
      481 gaagtcgggc gcggttacct ggagctgacg ggatactggc ggccgatgac gctctagcgc
      541 caggcccaca ccggctgctc gaggtgcgcg acgcgcgtcg cgcggccgag caccgcgact
      601 tccatgaact ggtacaggac cgccgcggtc ggcgcatgga tcagcgtgcc gagaagcgga
      661 tagcggatca ccgggcggtc gctctccggg atcgagacga actgcggcga gccgtgccgg
      721 ccgagctcgg caaggggcac gcggtagatc ttcgccacct cggcgggatt gggggcgagg
      781 cgcgcgccgt ccgccaccca ggcgacgagc ggggtgatca ggtagcccga gcgcgtcgcg
      841 tagtcgtcga gcgcgccgag aaacgcctgc ggcgcgagct cgaggccgat ctcctcgcgc
      901 gcttcgcggc gcgcggcggc gagcgtggat tcgccgccgt cgacgcgccc gccgggcagc
      961 gcgtactggc ccgaatgcgt gctcaggtgc tcggcgcggc gggtcagcac cagagccgcc
     1021 tcgccgcggc cgtcgtcggt gacgaggacg cacaccgcgg cgcgcttgag cccatgcggc
     1081 gtactcggcg ccggcgcgtg gcgcgacagg ttggtggcga ggcgcgcgcg caaggtggcg
     1141 tcgaacgcaa gcggcttcat gcggcggggg cgaaacgcac aggcggccat tatcgcctgc
     1201 atgctataaa tcgcgccgat gccctatgtc acctgcgccg gccatgcgct tgagtacgaa
     1261 tgggtcggcg ccgcggcgcc gggtgcgccg acgctggtct tcctgcacga ggggctgggc
     1321 tcgatccgcc agtggcgcga cttccctcag caggtggtcg atgccacggg cagccgcgcg
     1381 ctcgtctaca gccgttacgg ctacggccgc tccgacgtgc tcgccgagcc gcgcgtcggc
     1441 gtcgatttca tgcaccgcga agcgctcgac gcgctgcccg agctggtggc gcagctgggc
     1501 ctggagcggc cgatcctcgt cgggcattcg gacggcgcct cgatcgccct gatctacgcg
     1561 ggggcggggc acccggtgcg cgccgtggcg gcgatggcgc cgcacgtctt cgtcgagccg
     1621 atctgcgtcg acagcatccg cgcggcgcgc gcggcgttcg gcgcaaccga tctcgggcag
     1681 cgcctcggca aataccatcg cgacccgcgc aagacgttcc atctctgggc cgatgcctgg
     1741 ctcgacccgg agttcctgaa atggagcatc gaggaaatgc tgccgcagat cgcctgcccg
     1801 gtgctggcga tccagggcga ggatgacgag tacggcactc tggagcagct cgagcgcatc
     1861 cgacgcggcg tgcgcggaac gtgcgagatc gtgaagctgc cggcatgcgg ccacgcgccg
     1921 ttccgcgacc agccccaggc cgtgctcgac gctctgacgc ggttcgtcca cgagcatggc
     1981 cgctgacgtc ctgcgccagg acgtgaaggt gatcgggctg gtggcgctcg cccacgggct
     2041 gtcgcacttc ttccagatcg ccacggcggt gctgtttccg ctgatcaagg acgatctcgg
     2101 cgtgagctat accgcgctcg gcgccaccgt ggcgctgtac ttcacggtgt cgggtatctg
     2161 ccagacgctc gccggattcg ccgtggatcg cttcggcgca cgccgcgtgc tgttcttcgg
     2221 gctcgtgctc gcggtggccg gcgcgctggt ggccggcctg gcgcagaact attcgatgct
     2281 ggtcgccgcc gccgtggtcg gcggcctcgg caacagcgtg ttccacccgg tggatttctc
     2341 gatcctcaat gcccgcgtca ccgtgcgccg cctcggctac gccttcagct ggcacggcat
     2401 cgccgggtac ctgggctacg cctcagcacc ggcctatggc atcgccatgg cgagcgcttt
     2461 cggctggcgc ggcgcgctgc tcggcgcggc ggtgatcggc gcggcggtgg tcctgctgct
     2521 cacgctgcag cgggggtcgc tgcacgtgga gcctgcggac gcgcgccgcg cgcctgccgg
     2581 gctcggcgcc gacctgcgcg tgctcgcgca gctcccggtg gtgatggtgt tcggctattt
     2641 cgtcatgctg ggtgtggcct tcgtcggcat ccagacattc ggcatcgccg ccatgctttc
     2701 gctctacggc gtgaacgtgg cgctggcgtc ggccgcgctc accgcctacc tggtgggcgg
     2761 ggcggccggg atcttctgcg gcggcttcgt cgccgtgcgc gccacgcgcc acgacctcgt
     2821 ggccgtgagc ggcatggcgg cgagcgcggc gctcttcagc gtgctcgcca tgggctggct
     2881 gccgggaacg ctgctgccgc tgttgctggg agccgccggc ttcaccagcg ggctgaccaa
     2941 tccgtcgcgc gatctcatcg tacggggcgc gacaccggcg ggcgcgaccg gcaaagtgta
     3001 tggcttcgtg tattccggcc tggacgtcgg ctcgatggtc acgcccgtga tatttggctg
     3061 gctgctcgac cgcggcgagc cggcggtcgt gttctatgcc gtggtcggcg tgctggtgct
     3121 gtccatcctc acggtgttgc ggttaccgga gcgcgcgcct caaccggcgt aatcgacgcc
     3181 gcgacgctcg agcaggcgca gcaacgccgg ccagtccttg tgcgtggacg gccggtcgtc
     3241 gcgctggaac tgctcggtgg cggtatcggc ggcgctctgc gacatcagct gcacgttctc
     3301 caggcccgcc gccatggcgt cgacctggat ctggcaggcg cagtcgaggt aatacatcag
     3361 ctcgaaagcc tcgcgcacgg tctcgccgag cgtgagcagc ccgtggttgc gcaggatcat
     3421 gaccttggag cgctggccga ggctcgctgc catgcgctcg cgctcgctca agttgagcgc
     3481 gatgccctcg taatcgtggt aggtgcattg ctgctgcacg cgcatggcgt gctggctgat
     3541 gggcagcagg ccgcacttca tcgccgagac ggcgacgccg gcgcgcgtgt gcgtgtggat
     3601 gacgcagttg atctcgggcc gcgcctcgtg catgcaaccg tggatgacga agccggcctc
     3661 gttgtagccg agctcgagcg gatcgtcgag caccttgccg cgcgcgtcga tgcgcaccag
     3721 gttcgaggcg gtgatctcgt cgaacatcag cccgtaggca ttgatgagga agtcctcgct
     3781 gcccggcacg cgcgccgaga agtgcgtgta gatcaggtcg gtccacttga agagcgccgc
     3841 cagctggtag gcggcggcga gatccacgcg catctgccac tcctcgcggc tgacgcgctc
     3901 acggacgggc ttggagctct ttggcttgag gacggcgctc atggggtgtc tccttggagg
     3961 cggattctac tgccgcgagg gcctcgagca gcagcgccgg atcgttttgc gaaagccggg
     4021 cggagtccga gagcatgcgc cgccacaggc gcgcgcgcgg gtgaccgtgg tacagcccca
     4081 gcatgtggcg ggcgacgtgg cgcaggcccg gcgcccccgc ggcgtactcg gccatcgcgc
     4141 gcaccacttg cctgcgcgtc ttgccggggt cggcgaggag ccagctgtcg tggtaggccg
     4201 cgcggccgag catcaccccg tcgacgcgcg cgagatgggc gtcggcctgc tgccagccgg
     4261 tgataccgcc gttgatgacg atctcgagct gcgggaagtc ctgcttcagg cggtagacgt
     4321 aatcgtgctt gagcggcgga acctcccggt tttccttggg ggagagccct ttcaggacgg
     4381 cgttgcgcgc gtggacaata aacgtcgctg ccccggcccg cgagacgatg tcgacgaaac
     4441 gggccatgaa ctcataggct tctatccgat cgatccccgt gcggtgcttt atcgtcacgg
     4501 ggatcgaaac cgactcccgc atggcactga cgcattcggc caccagccca ggctcggcca
     4561 tcaagcacgc cccgaacgcc cccttttgca cccgctcgct cgggcaaccg cagttcaggt
     4621 tcacttcgtc gtagccgtac tcctgcacga gcttcgcgca acgcgcgaga tcctcgggct
     4681 cacttccgcc gagctgcgcc gccaccgggt gctcctcggc gttgaagccg aggtggcgct
     4741 cgacatcgcc gtgcaggagg gcgccggtgg tgatcatctc ggtatagagg aaagcgcgcg
     4801 ccgacacctg gcgcaggaag tagcggcagt gccggtcggt ccagtccatc atcggggcga
     4861 cgcacaagcg gtgagcgctc atcgggcgca agtgtagcgc gcgggtagaa tgcgacgcat
     4921 gcacaagatt ctcgtcaccg gcggcgccgg ctttctcggc tcgcacctct gcgagcggct
     4981 cctcggcgcg gggcacgacg tcatctgcct ggacaacttc tacacgggcg gcaaggcgaa
     5041 catcaagcac ctgctcggca ggcccgagtt cgagctgctg cgccacgacg tcacttttcc
     5101 gctgtacgtc gaagtcgagc agatctataa cctggcgtgc cccgcctcgc caatccacta
     5161 ccagcgcgac ccagtgcaga ccaccaagac cagcgtgcac ggcgcgatca acatgctggg
     5221 gctggccaag cgcacgcgcg cgcgcatcct gcaggcgtcc acctccgaag tctatggcga
     5281 cccggaggtg catccgcaag ccgagggcta ctggggccgc gtcaacccga tcgggccgcg
     5341 ctcctgctac gacgagggca agcgctgcgc cgagacgctg ttcttcgact actaccgcca
     5401 gcaccagctc gacatcaagg tggtgcgcat cttcaacacc tacggcccgc gcatgcaccc
     5461 gaacgacggg cgcgtggtgt cgaacttcat cgtgcaggcg ctgcgcggcg aggacctcac
     5521 gatctacggc aagggcacgc agacccgcag cttctgctac gtcgacgacc tgatcgatgc
     5581 gatggtgcgc atgatgggca ccgcgcgcgg cttcaccggg ccggtgaaca tcggcaaccc
     5641 gggcgagttc accatgctcg cgcttgcgga gatggtgctg cgcctcgtcg gcggcaagtc
     5701 gaagctcaag tacctgcccg cgcccgagga cgacccgatg cagcgccagc ccgacatcgc
     5761 gctcgccagg gacaagcttg gctgggcgcc gaaggtcgcg ctcgaggacg ggctgaagga
     5821 aaccatcgcc tatttccgca agacgctcgc cgcttgaaga tcttcgactg cgtcccgttc
     5881 ttcaacgagt tcgagcagct gcgcctgcgc attgcgctgc tcgaggacct ggtggaccgt
     5941 ttcgtggtgg tcgaggcgca tcagacgca
//
LOCUS       DBNHKE010000192        20228 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_510608, whole genome shotgun sequence.
ACCESSION   DBNHKE010000192 DBNHKE010000000
VERSION     DBNHKE010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 20228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_510608"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..577
                     /locus_tag="AC16O9_13110"
     CDS             224..577
                     /locus_tag="AC16O9_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6517591.1"
                     /translation="MEMVQAIEALSALAQETRLKVYRLLVEAGPAGLAAGRIGEELEL
                     PPATLSFHLAHLARAGLAKSRQDGRFVIYSADFQNMNALVAYLSENCCGRSACAPRAG
                     TVREKPWKAQKSRAS"
     gene            547..1374
                     /locus_tag="AC16O9_13115"
     CDS             547..1374
                     /locus_tag="AC16O9_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR6517592.1"
                     /translation="MESTEIKSIVRDRYARAATTVVAATKAASCCGGARTQAQSCDPI
                     TGNLYDAAQTGELPEAAVLASLGCGNPTALAELKPGETVLDLGSGGGIDVLLSAKRVG
                     PTGKAYGLDMTDEMLALAEQNRRRAGVQNVEFLKGDIENIPLPDRSVDVVISNCVINL
                     AADKDRVLREAFRVLRPGGRFAVSDVVTRGAVPDAIRSSMLLWVGCVAGALDEEEYRA
                     KLAAAGFRDIGVEPTRIYRLEDAKGIFEGKGIDVSAIAPQVEGKFMSAFVRATKPAA"
     gene            complement(1477..1662)
                     /locus_tag="AC16O9_13120"
     CDS             complement(1477..1662)
                     /locus_tag="AC16O9_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517593.1"
                     /translation="MHSPVTTPGRAPGVFFDMPGRAVLLLSLAGFASAAAVITGFAVS
                     YRLFRLVNGPLADRIGY"
     gene            complement(1671..2750)
                     /locus_tag="AC16O9_13125"
     CDS             complement(1671..2750)
                     /locus_tag="AC16O9_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6517594.1"
                     /translation="MRVPSVAQSVPQQKLLVAAVATACALPAAALAQLPAGVEVYGRA
                     NVSYERITVDSTPSVTNWEAVDNSSRIGVRGKKDLGDGLSGFFQVESRVKLDDGAGST
                     LSSRDSYLGMQGGFGTVRVGRTIGPVYYATYDYISNHNHDTGTSSDALLAPTVFGNQG
                     FMNNTVWYSSPKMGALSVDVAFSRLGENRVTAGMSQPSHLGIVGAYDQGPLHVAVSHA
                     ETKNSADLGGGTPNKDQATTIGGLYNFGGRYIVGALFESAKSDLLVGSAKRNYYRVSL
                     MMPVAKHEFHVNVGIADDRLDLNAADSGAKQWTLGYNYNIDKQFKVYAFYTVVDNETN
                     GNYGFKTNVAGVDNQSLAVGARYNF"
     gene            3144..4388
                     /locus_tag="AC16O9_13130"
     CDS             3144..4388
                     /locus_tag="AC16O9_13130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517595.1"
                     /translation="MFDFTLNETQRALRDEVRDFVKSVPRQLILDMDADKIDFPRDFL
                     REAGRRNLMGCRYAKRWGGRELDWVSTAMVMEEVGALGYIFACTFGVGAELVCDAIVL
                     HGNDAQKEKYVAPLLRGEIFAAECLTEPRSGSDFFGTTTVAEDRGDHFLLNGQKRFIV
                     GGTAADYFLVYARTDPHAEPHRGISCLLVDRGPGVSADYLYGLMGCRGGGASRVVFTD
                     VKVPKENLLGELHGGLAVFNTMMIPERLGTAAMTIGAARPALEVATTYTSRRKAFGQV
                     INRFQGVSFQIADAAMLLDASRAFVYSTCLAVQHEGASSRVRRLVSETKKFVTESCQQ
                     VVHNAMQVMGGIGYTNVFPIERIHRDLRLASIWTGTNEVLSAIASSEWYKEHAAAGRV
                     NATRDLEPDALESGAVDEKIYG"
     gene            4598..5392
                     /locus_tag="AC16O9_13135"
     CDS             4598..5392
                     /locus_tag="AC16O9_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6517596.1"
                     /translation="MSQPPAVLRDLDARGVATVTLNRPDVNNAYDETLIGGLHEAMDE
                     LGAHPGLRVVVLRGTGRHFQAGADLKWITRVAGQSAEENERVSRATASAVHRLNTLPV
                     PTVALVQGGCFGGGTGIVAACDVVIAADNAMFSIAETRWGLMAGIILPQLADAIGARQ
                     VRRYALTGERFDAHEARRIGLAHEVVPLADLPAAGERVVAQLLQNAPEANAQTKEVAL
                     EFAWGGIGEAVLGRLVAQHAAKRCSAEAAEGLASFVEKRPARWGAG"
     gene            5432..6875
                     /locus_tag="AC16O9_13140"
                     /pseudo
     CDS             5432..6875
                     /locus_tag="AC16O9_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            complement(6872..7729)
                     /locus_tag="AC16O9_13145"
     CDS             complement(6872..7729)
                     /locus_tag="AC16O9_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR6517597.1"
                     /translation="MIELVIDPRRRDLGGFEVGRLLPFTARRMIGPFIFLDRMGPAAF
                     APGIPRSVDVRPHPHIGLSTLTYLFEGEIMHRDSVGSEQAIRPGEVNWMVAGRGITHS
                     ERFERARREGGRMDGLQAWVALPREDEETEPAFSHHGVQDLPTLDEGGVARRLIAGEA
                     YGARAPVRTHSPLFYVHSTLAAGAREQLPAGFAERAAYVIKGEVEAEGRRFKEGQLLV
                     FGAGAAVLEARTPAALMLLGGEPVGERFIEWNFVSSSKARIEQAKADWRAGRMKLPDL
                     DDQEFIALP"
     gene            complement(7741..8493)
                     /locus_tag="AC16O9_13150"
     CDS             complement(7741..8493)
                     /locus_tag="AC16O9_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR6517598.1"
                     /translation="MQKFAAICFCLGLGAAGAAAAQTVAKPDGAWRGSLGANVSSASG
                     NNESLNAGLNADVVRQTEVDKLSGFVQSLYGRRETGGVSELTASRFRAGTRYDRDITD
                     LTYGFIGYDVEKNKLNDLKWRHSPSIGAGLHLRKTETFTFNVFAGYSYNRERLYSGGK
                     RSFDEALIGEETTHQFSESTKFRQRLSYYPNLSDSGEYRVVLDAGLLTSLVDRWNLTV
                     NLSARYQSNPPLGVQKSDTLLFTGLQYSWGPK"
     gene            8588..10018
                     /locus_tag="AC16O9_13155"
     CDS             8588..10018
                     /locus_tag="AC16O9_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517599.1"
                     /translation="MHDTSGTIRTRIPARLDQLPWSRFHWLVVLALGVTWILDGLEVT
                     FKGAVSGVLQLPETMGFSAAEIGLLGSGYLTGAVCGALVFGRLTDRYGRKRLFFITLG
                     VYLAGVGLSAFSWDFWSFFVFRFITGCGIGGEYAAINSAIDELIPARHRGRVGLMING
                     SFWLGAALGSGATVLLLDPARFAPDLGWRLGFGIGAALGLAILFFRRFIPESPRWLLT
                     HGRAAEAERIVAGIERSIGAPPPPADVEFVLRPHRRFGLVQVAHVMFRRYRSRSALCV
                     MLMMSQAFLYNAIFFTYALVLTRFYAVPAGDTGLYLLPFAIGNFLGPVVLGRWFDTVG
                     RRPMIALTYGTSATLLALTGWLFTQDLLSAAGQTAMWSVIFFFASAAASSAYLTASEL
                     FPLEIRALAIAIFFAVGTGTGGILAPWLFGTLIGSGSRDAVFAGYLLGAGLMLLAVGA
                     VLAYGVRAERKSLEEVAPPLSSVSDR"
     gene            10093..10866
                     /locus_tag="AC16O9_13160"
     CDS             10093..10866
                     /locus_tag="AC16O9_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HIR6517600.1"
                     /translation="MKRNMLLVLMLAALGLGGCATQFEARPESEAPTLRFQGVSIQPD
                     DTAERIAPAELRPGDILLTSTGTLRSAGIQLMTFAPVSHAAVYVGRGQVVEAVGSGVR
                     VRTLRALLEEESIALALRYPDLSELQAQSVRSVALEKVGRNFNYVGVAMHVPFTINRR
                     LCELPLLPAAARDLCLQSMGGVFHLATSRRALFCSQLVLQSYRQAGVPLTDADPRLIS
                     PADILHMREGDVPSVRVKRPLRFLGHLKYEVAAAVPLAH"
     gene            complement(10863..11639)
                     /locus_tag="AC16O9_13165"
     CDS             complement(10863..11639)
                     /locus_tag="AC16O9_13165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6517601.1"
                     /translation="MHPMLNIAVKAARRAGALINRAALEGGLEVRAKNRNDFVTQVDK
                     AAEQAIIDVIRRAYPEHGFLAEESGASAGARPEFLWVIDPLDGTTNYIHGFPQYCVSI
                     ALEHKGVLTQAVVYDPAKNELFTASRGRGAFLDDRRMRASKCTQLAGALVGTGFPFKE
                     LDHIDAYMRQLRTVMSTAAGVRRAGAAALDLAYVAAGRLDAFWEMGLSRWDMAAGALL
                     IQEAGGLVSDLDGKAGFLDSGEIAAAAPKVMPQLLDALRA"
     gene            11661..12353
                     /locus_tag="AC16O9_13170"
     CDS             11661..12353
                     /locus_tag="AC16O9_13170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR6517602.1"
                     /translation="MSAPRIVLCRPSHPGNIGAAARAMKTMGLADLRLVAPARPPAPQ
                     DAWMATHGADVLAAAQTHATLGEAIADCAAAFALSARPREWSPQVLDARTAAARAATF
                     AEPVAFVFGSEDAGLTNEELFACQFLVHIPADPESSSLNLAQAVQVVCYELRMAQGAA
                     VPPMRTEKLATVADLEGLYAHLEEVARASGFMAPGSKLRERWRRLFSRVPGLEREEVN
                     ILRGLLKSLYKK"
     gene            12389..13093
                     /gene="cysE"
                     /locus_tag="AC16O9_13175"
     CDS             12389..13093
                     /gene="cysE"
                     /locus_tag="AC16O9_13175"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR6517603.1"
                     /translation="MFKHLREDIASIFARDPAARTTFEVLTCYPGLHAIYIHKLARWF
                     WTHGLRWLGRFTSHVGRWATGIEIHPGVIIGRRVFIDHGMGVVIGETAEVGDDCTIYQ
                     GVTLGGTSLYRGAKRHPTLGAGVVVGAGAKVLGGFTVGAGAKIGSNAVVVTAVPAGAT
                     VVGIPGRIIEEATAQKAPHFAAYAVEQGQDDPFAQEIQKLAEHSAELEQALAKLKEKL
                     ADLEKDQRRDQLRAVK"
     gene            13159..13626
                     /gene="iscR"
                     /locus_tag="AC16O9_13180"
     CDS             13159..13626
                     /gene="iscR"
                     /locus_tag="AC16O9_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HIR6517604.1"
                     /translation="MRLTTKGRFAVTAMVDLAMRQSRGPVALAAISERQHISLSYLEQ
                     LFGKLRRRKLVSSVRGPGGGYNLAQPAADVTVADIVTAVDEPLDATQCGGKENCADEK
                     RCMTHDLWATLNSKMYEYLSSVSLADLVAHQGGKHIAVLHDHRRAPDKEEVAA"
     gene            13623..14831
                     /locus_tag="AC16O9_13185"
     CDS             13623..14831
                     /locus_tag="AC16O9_13185"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR6517605.1"
                     /translation="MKLPIYLDYSATTPVDPRVAQKMIPYLTENFGNPASRSHAFGWK
                     AEEGVEQARAHVAALIGADPKEIVWTSGATEGNNLAIKGAANFYKTKGKHLVTQKTEH
                     KATLDTMRELERQGFECTYLDVEENGIVDLKKLEAALRPDTILVSIMMVNNEIGVIQP
                     IAEIGELCRARGIIFHCDAVQAAGKLPIDLGTLKVDLLTITAHKMYGPKGIGALYVRR
                     KPRVRIEPQIHGGGHERGFRSGTLATHQIVGFGEAARLAKLEMATDNERIRALRDRLW
                     RGLSSMEAVVLNGDLERRIPGNLNVSFNYVEGESLIMAIKDIAVSSGSACTSASLEPS
                     YVLRALGRSDELAHSSIRFTLGKFTTEEEVDYAVNLVQAKVAKLRELSPLWEMYKDGV
                     DLNTVQWAAH"
     gene            14846..15262
                     /gene="iscU"
                     /locus_tag="AC16O9_13190"
     CDS             14846..15262
                     /gene="iscU"
                     /locus_tag="AC16O9_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIR6517606.1"
                     /translation="MAYSDKVIDHYENPRNVGSFDKGDEAVGTGMVGAPACGDVMKLQ
                     IRVGKDGVIEDARFKTYGCGSAIASSSLVTEWVKGKRLEEAAALKNTQIAEELSLPPV
                     KIHCSILAEDAIKAAIADYRKKHGLEAAQEQKEKVA"
     gene            15264..15587
                     /gene="iscA"
                     /locus_tag="AC16O9_13195"
     CDS             15264..15587
                     /gene="iscA"
                     /locus_tag="AC16O9_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HIR6517607.1"
                     /translation="MAVTLTEKAATHVQDYLAKRGKGVALRVGVRTSGCSGMAYKLEF
                     ADAVNPEDHQFESHGVKLVVDPKSLPFLEGTQLDYTREGLNEGFKFLNPNVKDECGCG
                     ESFNV"
     gene            15584..16090
                     /gene="hscB"
                     /locus_tag="AC16O9_13200"
     CDS             15584..16090
                     /gene="hscB"
                     /locus_tag="AC16O9_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HIR6517608.1"
                     /translation="MKDHFALFGLAPAFAVDAAALEGAYRDIQSRIHPDRHAHAGDAE
                     RRAAMQWTTRVNEAYRTLKSPVQRARYLLEMRGVDVGLETNTQMPAEFLTRQLELREA
                     LAEATGPGALDRLHDTVRAQTRDLEQALADLIDVRRDYAAAAGQVRKLMFLHKLAEEI
                     DRAYEENE"
     gene            16090..17958
                     /gene="hscA"
                     /locus_tag="AC16O9_13205"
     CDS             16090..17958
                     /gene="hscA"
                     /locus_tag="AC16O9_13205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HIR6517609.1"
                     /translation="MALLQISEPGRSPAPHQRRVAVGIDLGTTNSLVAAVRSGVPVVL
                     ADGLGRPLLPSVVRYHRDGRVDVGYAAQARQAEDPRNTVVSVKRYMGRGLKDVVHAEN
                     APYDFVDAPGMVQLRTVAGTKSPVEVSAEILKVLRQRAEFALAGSLDGAVITVPAYFD
                     DAQRQATKDAGQLAGLNVLRLLNEPTAAAIAYGLENAAEGVYAVYDLGGGTFDISILR
                     LSKGVFEVMATAGDAALGGDDFDQRVFCWVVEEAKLPPLGAKDMRLLLVKSREAKEYL
                     TAHGEARVTAKLSTGDVVDVSLDTEAFSGVTQHLVQKTLGPMRRALRDAGLRPDQVKG
                     VVMVGGATRMPQIQRAVGEFFRCDPLNNLDPDKVVAIGAAMQANVLAGNKPAGEDWLL
                     LDVIPLSLGVETMGGLAEKIIPRNSTIPVARAQEFTTYKDGQTGMSFHVVQGERELVA
                     DCRSLARFELKGIPPMAAGAARVRVTYQVDADGLLSVQAQEATTGVEASVAVKPSYGL
                     ADGEIERMLRDSFEHARDDMHARALHEARVDAARLLEATRSALAADGALLEPHERAEI
                     EARVAALDSALAGSDHRALRAGSDALNRATEEFAARRMDAGVRRALAGRKIATLDL"
     gene            17959..18306
                     /gene="fdx"
                     /locus_tag="AC16O9_13210"
     CDS             17959..18306
                     /gene="fdx"
                     /locus_tag="AC16O9_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HIR6517610.1"
                     /translation="MARIKVLPHAELCPQGAAFEAPAGVSICDALLEHGVDIEHACEK
                     SCACTTCHVILRQGFDSLDAAEDKEEDLLDKAWGLEATSRLSCQARVADADLTVEIPK
                     YTINYEREGGGRK"
     gene            18303..18494
                     /gene="iscX"
                     /locus_tag="AC16O9_13215"
     CDS             18303..18494
                     /gene="iscX"
                     /locus_tag="AC16O9_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR6517611.1"
                     /translation="MKWTDTLDIAIALSEKYPEVDPKTVRFTDLYQWVQQLEGFDDDP
                     KRCGEKILEGIQMAWIEES"
     gene            18494..18886
                     /locus_tag="AC16O9_13220"
     CDS             18494..18886
                     /locus_tag="AC16O9_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517612.1"
                     /translation="MGVLGERLARAFAGRRVLVLGGGTQWSGSFSGKAAEVTVQEGLD
                     ALPDFGRRHDALFAPEVWSRLPSERLDEFLRRAVQAAAPGALIAFLDRRDGTPLEGEL
                     IGRASRHGWGANLELLPDYWLLTWWAPR"
     gene            complement(18900..19493)
                     /gene="msrA"
                     /locus_tag="AC16O9_13225"
     CDS             complement(18900..19493)
                     /gene="msrA"
                     /locus_tag="AC16O9_13225"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6517613.1"
                     /translation="MNRLLLLLCATLLPALAQAQGLAKATFAAGCFWCTEEAFDKVPG
                     VLSTVSGYMGGHIKEPSYALVSTGRSGHTEVLQVTYDPKQVSYDKLLETFWLNHDPTA
                     LDHQFCDHGSQYRPGIFHHDAEQKRLAEASKARWEKDKPFKDAIVTPIEAASDFWPAE
                     DYHQDYYKKNPARYRFYVTGCGRYARLDQLWGKYRAK"
     gene            complement(19540..>20228)
                     /locus_tag="AC16O9_13230"
     CDS             complement(19540..>20228)
                     /locus_tag="AC16O9_13230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6517614.1"
                     /translation="AIPRARLEYLMRQQLQRGAPDNEQTRALVREDLINRELVSQVAA
                     RAGTAKKREVQTQLEVARQEVLVGAYLSDYLRENAVTDADVKKEYDRVRDQTGANEYK
                     ARHILVETEDEAKRLIADLQKGDKFDELAKKSSKDEGTRELGGDLDWKIPSTLEKPFA
                     DAMVALEKGKFTGTPVRTRFGYHVIQLDDVRAVDFPPLAQVRPQIQQRLARQKVEELV
                     RELRAKAKVE"
BASE COUNT         3209 a         6690 c         7107 g         3222 t
ORIGIN      
        1 gggcggcgag ggcgcgattc ggggctcaag gggcgaaggt ccgaccggcg cgcatgcagg
       61 gcgcgcccag catcggcggg acaatcggcg ttgtggtcaa taacagcgac ttccgcatcg
      121 agccgggccg gagccgttga gttgtcattg tcggcgtagt gcgtgcgttc gcgctccggg
      181 gcgagttctt gacatcgcgc ttgatttcga gtattctaga aacatggaaa tggttcaggc
      241 gatcgaagcg ctctctgcgc tcgcacagga gacccggctg aaggtatatc ggctcctcgt
      301 tgaagcgggg ccggcgggtc tggccgccgg gcgcatcggc gaggagctgg aactgccccc
      361 tgcgacgctg tcctttcacc tcgcgcatct tgcgcgggcg gggttggcga aaagccggca
      421 ggacgggcgc ttcgtgatct attccgccga cttccagaac atgaatgcgc tggtcgccta
      481 cctttcggag aactgctgcg ggcgcagcgc ctgcgctcca cgcgccggga cagtacggga
      541 gaagccatgg aaagcacaga aatcaagagc atcgtaaggg accgctatgc gagggccgcg
      601 acaaccgtcg tcgctgcgac caaagccgcc tcctgctgcg ggggagcgcg cacacaggcg
      661 caatcctgcg atccgatcac gggcaacctg tacgacgcgg cgcagaccgg cgagctacca
      721 gaggccgcgg tgctggcgtc gctcggttgc ggcaatccga ctgcgctggc cgagctgaag
      781 ccgggggaga ccgtcctcga cctgggctcg ggcggcggca tcgacgtgct gctctccgcg
      841 aagcgcgtcg gtccgacggg caaggcctat gggctcgaca tgaccgacga gatgctggcg
      901 ctcgccgagc agaacaggcg cagggccggg gtgcagaacg tcgagtttct gaagggcgat
      961 atcgagaaca ttccgctgcc cgaccgctcc gtggacgtcg tcatctccaa ctgcgtgatc
     1021 aaccttgccg ccgacaagga ccgcgtgctg cgcgaagcct tccgtgtcct caggccggga
     1081 ggccggttcg cggtgtccga cgtggtcacg agaggtgcgg tgcctgacgc catccgcagc
     1141 agcatgctgc tctgggtagg ctgcgtcgcg ggtgcgctgg acgaagagga gtaccgcgca
     1201 aagctcgccg ctgccggatt ccgggatatt ggcgtcgagc cgacccggat ctaccgcctc
     1261 gaagatgcga agggcatttt cgagggcaag ggcatcgacg tgagcgcgat cgcgccgcag
     1321 gtcgagggca agttcatgag cgccttcgtc cgcgcgacca agccggcggc ctgaccggtt
     1381 cttctctcgc ggcgcgcttc gggggtcgcg ggctgaatgc tcgatcggcg cgcatgaagg
     1441 gcgcgcccag gatcggcggg acaatcggcg ttatggtcaa taaccgatgc gatcggcgag
     1501 cggcccgttg acaagtcgaa agaggcgata agagacggcg aagccggtga tcaccgccgc
     1561 ggcgctggcg aagcccgcaa gagacaggag caggacggcg cggccgggca tatcaaaaaa
     1621 aaccccgggc gcgcggcccg gggtggtgac aggcgaatgc aaacggcgcg tcagaagttg
     1681 tagcgcgcgc ctacggcgag cgactggttg tcgacgcccg cgacattggt cttgaagccg
     1741 tagttgccgt tggtctcgtt gtcgacgacc gtatagaagg cgtagacctt gaactgcttg
     1801 tcgatgttgt agttgtagcc gagggtccac tgcttggcgc cggaatccgc cgcattgagg
     1861 tccaggcgat cgtcggcaat gccgacgttg acatggaact cgtgcttggc caccggcatc
     1921 atcagcgaga cgcgatagta gttgcgcttc gcgctgccga ccagcagatc gctcttcgcg
     1981 ctctcgaaca gcgcaccgac gatgtacctg ccgccaaagt tgtacaggcc gccgatggtc
     2041 gtcgcctgat ccttgttcgg cgtgccgccg ccgaggtccg cggagttctt cgtctccgca
     2101 tgcgacacag cgacatgcag cggtccctgg tcgtaggcgc cgacgatccc taggtggctc
     2161 ggctgcgaca tgccggcggt aacgcgattc tcaccgagcc gggagaacgc cacatccacc
     2221 gagagggcgc ccattttcgg gctcgagtac cacaccgtgt tgttcatgaa accctgattg
     2281 ccgaagacgg tgggcgcgag cagtgcatcg gaagacgtgc ccgtgtcatg gttgtggttg
     2341 ctgatgtagt cgtaggtggc gtaatacacc gggccgatgg tgcgccccac ccgcacggtg
     2401 ccgaatccgc cttgcatgcc gaggtagctg tcgcggctgg acagggtgct gcccgcgccg
     2461 tcgtccagct tgacccggct ctcgacctgg aagaatcccg acaggccatc gcccaggtcc
     2521 ttcttgccgc gcacgccgat gcgcgagctg ttgtcgaccg cctcccagtt cgtcacgctg
     2581 ggggtactgt cgacggtgat gcgctcgtag ctgacattgg cgcggccgta gacctcgact
     2641 ccggccggca actgcgccag ggccgccgcc ggcagcgcgc aagcggtcgc gactgcggca
     2701 acgaggagct tctgctgcgg gacggactgc gcaacgcttg gaactcgcat aacggttttc
     2761 ctttcgttcc tagttggaac tcgaagcaaa taccacccgg gcgcgcggcg cacgccgccg
     2821 cgcatcatcc cacaaaagag tggtgttacg gcattcccga accatgcagc gaccagggaa
     2881 atttcagcga ttggcgcgct gcaaaagact cgtcgagccg gtcgcgttgc atgccggcgg
     2941 caccggtacg gatgaaaaag gaagggcttg cgccctccgc ttttctaatg gtgcagcggc
     3001 gctttcgggg ccgcggcggg gcgatgcagc gggttggcca ctgcgcagga tcggccgggc
     3061 gcggtgcccc cttttgagcc tctccccata cccgcccgtc gaggcatagg atagaaagaa
     3121 cacacccgag gccggaggct gcgatgttcg atttcacgct caatgagacc cagcgcgcgt
     3181 tgcgcgacga agtccgcgac ttcgtcaagt cggtgccgcg ccagctgatc ctcgacatgg
     3241 atgccgacaa gatcgatttc cctcgcgatt ttctgcgcga ggcgggccgc cgcaacctga
     3301 tgggctgtcg ctatgcgaag cgctggggcg gtcgcgagct cgactgggtc agcaccgcga
     3361 tggtgatgga ggaggtcggc gcgctcggtt acatattcgc ctgcacgttc ggcgtcggcg
     3421 ccgagctggt gtgcgacgcc atcgtcctgc acggcaacga tgcgcaaaag gaaaagtatg
     3481 tcgcgccgct gctgcgcggc gagatcttcg ccgccgagtg tctcaccgag ccgcgcagcg
     3541 gctccgattt cttcggtacc acgaccgtgg ccgaggaccg cggcgatcac ttcctgctca
     3601 acggccagaa gcgcttcatc gtcggcggga cggcggccga ttacttcctg gtctatgcgc
     3661 gcaccgatcc gcacgccgag ccgcaccgcg ggatctcctg cctgctggtg gatcgcggtc
     3721 ccggcgtctc cgccgactat ctttacggac tgatgggatg ccggggaggg ggcgcgagtc
     3781 gcgtcgtgtt tacggacgtg aaagtcccga aggagaatct gctcggcgag ctgcacgggg
     3841 gtctcgccgt gttcaatacc atgatgatcc cggagcggct tggcaccgcg gcgatgacca
     3901 tcggcgccgc gcggccggcg ctcgaggtcg cgaccaccta caccagcagg cgcaaggcgt
     3961 tcggccaggt gatcaaccgc tttcagggcg tcagcttcca gatcgctgac gccgcgatgc
     4021 tgctcgacgc ctcgcgcgcc ttcgtctaca gcacctgtct cgcggtgcag cacgagggcg
     4081 cctcgtcacg cgtccgccgc ctggtgtcgg agaccaagaa gttcgtgacc gagtcctgcc
     4141 agcaggtggt gcacaacgcc atgcaggtga tggggggcat cggctatacc aacgtgtttc
     4201 cgatcgagcg cattcatcgc gacctgcgcc tggcgtcgat ctggaccggc accaacgaag
     4261 tgctttccgc cattgccagc agcgagtggt acaaggagca cgctgccgcc gggcgcgtca
     4321 acgcgacccg cgatctcgag cccgacgccc tcgagtcggg cgcggtggac gaaaagatct
     4381 acggatgagg cgctccggct ccggctgagg aacgacccgg ccttgcgcgc ggtgctcgat
     4441 cgttggcaac tcttctgcat tcgttcgacg gcggtgagca ctcatggtgc tcccgcccac
     4501 tactgctgca tcatcagcgt atggttgata aagtcctgtc gttccaagca actcgatcat
     4561 caccgacagc cgaaatcctc aagcgaagga agacgtcatg agccaaccgc cagccgtact
     4621 gcgagacctc gacgcccgcg gcgtcgccac cgtgacgctg aaccgccccg acgtgaacaa
     4681 cgcctatgac gagacgctga tcggcggcct gcacgaagcg atggacgagc tgggcgcgca
     4741 tccgggcctg cgcgtcgtgg tgctgcgcgg caccggccgg cacttccagg cgggcgccga
     4801 cctgaaatgg atcacgcggg tcgcgggcca gtcggccgag gaaaacgagc gcgtctcgcg
     4861 cgccacggcg agcgcggtgc accgcctgaa cacgctgccg gtgccgacgg tggcgctggt
     4921 gcagggcggc tgcttcggcg gcggcaccgg catcgtcgcc gcctgcgacg tggtgatcgc
     4981 cgcggacaac gcgatgttct cgatcgccga gacgcgctgg ggcctgatgg ccggcatcat
     5041 cctgccgcag ctcgccgatg ccatcggcgc gcgccaggtg cgccgctacg cgctgacggg
     5101 cgagcgcttc gacgcgcacg aagcgcggcg catcggcctc gcgcacgagg tggtgccgct
     5161 cgccgacctg ccggccgcag gcgagcgcgt ggtcgcgcag ctgttacaga acgcgccgga
     5221 ggcgaacgcg cagaccaagg aggtggcgct ggagttcgcg tggggcggga tcggcgaagc
     5281 ggtgctcggc cggctcgtcg cgcagcatgc ggcgaagcgc tgcagcgcgg aagcggccga
     5341 aggcctcgcg tcgttcgtcg agaagcgccc cgcgcgctgg ggcgcgggtt agtatcgcgc
     5401 tctccaagat ccgtaacgaa cgaggaccgc catgggatac atcgacaccc aactgttcat
     5461 cgacggcaaa tggcagcagc ccgcggaagg caagtcgctg cccgtcgtca atcccgccac
     5521 cggcaaggag atcgggcgcg tcgcgcatgc cggccggccc gatctcgacc gcgcgctcca
     5581 ggcggcgcag aagggcttcg aagtctggcg cgacatggtg cccgccgagc gcagcaagat
     5641 catgcgcaag gccgccgcgc tgatgcgcga gcgcgcgggc gagatcgcgc cgctgctcac
     5701 gcaggagcaa ggcaagccgc tggcggaagc caaggccgag acgctggcgg ccggcgacat
     5761 catcgactgg ttcgccgagg agggctttcg cgtctacgga cggctcgtgc cttcgcgcat
     5821 gagcgtcacg atccggcaga tggtcatgaa ggaccccgtc ggtccggtcg ccgccttcac
     5881 gccgtggaat tttccgatca accaggtggt gcgcaagatc ggcgccgggc tcgcggccgg
     5941 gtgctcgatg ctcgtcaagg cgcccgaaga gacgcccgcc gcgccggcgg cgctgatcaa
     6001 ggccttcgcc gacgccggca tcccgcccgg cgtcctgcag ctcgtgtacg gtgcgcccgc
     6061 cgagatctcg agctacctca ttccgcaccc ggtgatccgc aagatcacct tcaccggctc
     6121 gatcgcggtg ggcaagctgc ttgcccaccg cgatggctgg ccagcacatg aagcgcgtca
     6181 ccatggaact tggcggccac gcgccggtga tcgtgtgcga ggacgccgac atcgcgctcg
     6241 cgctcaaggc agcggcggcc gcgaagttcc gcaacgcggg ccaggtctgc atttcgccca
     6301 cgcgcttcct cgtgcacaag tcgatcgccg gcgatttcgc gcaggcgctg gccgactacg
     6361 ccaaggggct gaaggtcggc gacggtctgg ccgagggcac gcagatgggt ccgctcgcca
     6421 acccgcgccg cgtcgaggcg atgaccgcgt tcaccgagga cgcggtgaag aagggcgcga
     6481 agctccttgc cggcgggcag cgcatcggca aggccggcaa cttctgggag ccgaccatcc
     6541 tcgccgacgt gccgaaggag gcgaaagtct tcaacgacga gccgttcggc cccatggctg
     6601 cgatccggcc gttcgagtcg ctggacgagg cgatcgccga gtcgaaccgc ctgccctacg
     6661 ggcttgccgg ctacgccttc acgcgctcgc tcaagaactc ggacctcctt gcgcgccggc
     6721 tggaagtcgg catgctgtgg atgaacatgc cggcgctgcc ctcggccgag atgccctttg
     6781 gcggggtgaa ggactcgggc tacggctccg aaggcggccc cgaggcgctc gaggcgtacc
     6841 tcaactcgcg cgcggtggcg atccagaacg cctagggcag cgcaatgaac tcctgatcgt
     6901 ccagatcggg aagcttcatg cgccccgcgc gccagtcggc cttggcctgc tcgatgcgcg
     6961 ctttcgacga ggatacgaag ttccactcga tgaagcgctc gcccaccggc tcgccgccga
     7021 gcagcatcag cgccgcgggc gtgcgcgctt cgagcaccgc ggcgcccgcg ccgaatacca
     7081 ggagctggcc ttccttgaag cgccgccctt cggcctcgac ctcgcccttg atgacgtagg
     7141 cggctcgctc ggcgaacccc gccggcagct gctcgcgcgc accggcggcc agcgtgctat
     7201 ggacatagaa cagcggggaa tgcgtgcgca cgggtgcgcg cgcgccgtag gcttcgccgg
     7261 cgatgagccg gcgcgcaacg ccgccttcgt cgagggtcgg gagatcctgc acgccatggt
     7321 gggaaaaggc gggctcggtc tcctcgtcct cgcgcggcag cgccacccag gcctgcaggc
     7381 cgtccatgcg gccgccctcg cgccgcgcgc gctcgaagcg ctccgaatgc gtgatgccgc
     7441 ggccggcgac catccagttg acctcgcccg ggcggatcgc ctgctcggac ccgacgctgt
     7501 cgcggtgcat gatctcgccc tcgaacaggt aggtcagcgt cgaaaggcca atgtgggggt
     7561 gcgggcgcac gtccaccgag cgcgggatgc ccggcgcgaa ggcggcgggc cccatgcgat
     7621 cgaggaagat gaacgggccg atcatgcgcc gcgcggtgaa cggcagcagc cgcccgactt
     7681 cgaagccgcc gagatcgcgg cggcgcgggt cgatgacgag ctcgatcatg gtcgcgcagc
     7741 ctacttcggc ccccagctgt actgcagacc ggtgaacagc agcgtatcgc tcttttgcac
     7801 gccgagcggc ggattgctct gatagcgcgc cgagaggttg accgtcaggt tccagcgatc
     7861 gacgagcgac gtgagcagcc cggcgtcgag cacgacgcgg tactcgcccg agtcggacag
     7921 gttggggtag tacgacaggc gctggcggaa cttcgtgctc tccgagaact ggtgcgtggt
     7981 ctcctcgccg atcagcgcct cgtcgaacga gcgcttgccg cccgagtaga gccgttcgcg
     8041 gttgtaggag tagccggcga agacattgaa ggtgaaggtt tcggtcttgc gcaggtgcag
     8101 gcctgcgccg atcgaaggcg agtggcgcca cttgaggtcg ttcagcttgt tcttctcgac
     8161 gtcgtagccg atgaagccgt aggtgaggtc ggtgatgtcg cggtcatagc gcgtgccggc
     8221 gcggaaccgg ctcgcggtga gctcgctcac gccgccggtc tcgcgccggc catacaggct
     8281 ctgcacgaat ccgctcagct tgtcgacctc ggtctggcgc acgacgtcgg cattgaggcc
     8341 ggcgttgagg ctttcgttgt tgccgctcgc cgagctgacg ttcgcgccga gcgagccgcg
     8401 ccacgcgccg tcgggcttgg cgacggtctg cgctgcggcg gcgcccgccg cgccgagccc
     8461 gagacaaaag catatcgctg caaacttctg cattcgcttc actcctttac cgggggtccc
     8521 agactcctga cctgcatcgg gcgcgatggt tccgcgcgac ggcgcactcc ggtataaccc
     8581 gtcgtacatg cacgacacaa gcggcacgat acgcacgcgc attccggcgc ggctcgacca
     8641 gctgccctgg agccggttcc actggctggt ggtgctcgcg ctcggcgtca cctggattct
     8701 cgacgggctc gaggtcacct tcaagggcgc ggtgagcggc gtgctgcagc tgcccgagac
     8761 gatgggcttc agcgccgcgg agatcggcct gctcggctcg ggctacctga cgggcgcggt
     8821 gtgcggcgcg ctggtgttcg ggcgcctgac cgaccgctac ggcaggaagc gcctgttctt
     8881 catcacgctc ggcgtgtacc tcgcgggcgt cggcctgagc gcgttctcgt gggacttctg
     8941 gagctttttc gtcttccgct tcatcaccgg ctgcggcatc gggggcgagt acgcggcgat
     9001 caactcggcc atcgacgagc tgatccccgc gcgccaccgc ggccgcgtcg gcctgatgat
     9061 caacggcagc ttctggctcg gcgccgcgct cggctcgggc gccaccgtgc tgctgctcga
     9121 cccggcgcgc tttgcgcccg atctcggctg gcgcctgggc ttcggcatcg gcgcggcgct
     9181 cggcctcgcg atcctctttt tccgccgctt cattccggag agcccgcgct ggctgctgac
     9241 gcacgggcgc gcggcggagg ccgagcgcat tgtcgcgggc atcgagcgct cgatcggcgc
     9301 gccgcctccg cccgccgacg tcgagttcgt gctgcgcccg caccgccgct tcggcctcgt
     9361 gcaggtcgcg cacgtgatgt tcaggcgcta ccgctcgcgc tcggcgctgt gcgtgatgct
     9421 gatgatgtcg caggccttcc tgtacaacgc gatcttcttc acctacgccc tcgtcctgac
     9481 gcgcttctac gccgtgccgg ccggcgacac cgggctctat ctgctgccct tcgccatcgg
     9541 caatttcctc ggcccggtgg tgctggggcg ctggttcgac accgtcggcc gccggcccat
     9601 gatcgcgctc acgtacggaa cgtcggcgac gctgctcgcg ctcaccggct ggctgttcac
     9661 gcaggatctg ctctcggcgg cggggcagac ggcgatgtgg agcgtgatct tcttcttcgc
     9721 ctcggccgcc gcgagctccg cctacctgac ggcgagcgag ctgtttccgc tcgagatccg
     9781 cgccctcgcc atcgccatct tcttcgcggt gggcaccggc accgggggca tcctggcccc
     9841 gtggctgttc ggcacgctca tcggcagcgg ctcgcgcgac gcggtgttcg ccggctacct
     9901 gcttggcgcg gggctcatgc tgctcgcggt gggggccgtc cttgcctacg gcgtgcgcgc
     9961 ggagcgcaag tcgctggagg aggtggcgcc gccgcttagc tctgtaagcg accggtaacc
    10021 ccccggtaag ccatcctcag cccgggctag tccatgctgg gtaggaacga caaccgtcaa
    10081 gcgggggtgg ccgtgaaacg gaacatgctg ttggtgctga tgcttgcggc gctcgggctc
    10141 gggggctgcg ccacgcagtt cgaggcgcga cccgagtcgg aggcgccgac gctgcgcttc
    10201 cagggcgttt cgatccagcc cgacgacacg gcggaacgca tcgcgccggc cgagctgcgg
    10261 cccggggaca tcctcctcac gtcgaccggg acgctgcgct cggcgggcat ccagctgatg
    10321 accttcgcgc cggtcagcca tgcggccgtc tatgtcggca ggggccaggt ggtggaggcg
    10381 gtgggctcgg gcgtgcgcgt gcggacgctg cgcgcgctcc tggaggagga atcgatcgcg
    10441 ctggcgctgc gctatccgga tctgtccgag ctgcaggcgc agagcgtacg ttccgtggcg
    10501 ctggagaagg tgggacgcaa tttcaactac gtgggtgtgg cgatgcacgt cccgttcacc
    10561 atcaaccggc ggctgtgcga gctgccgctg ttgcccgcgg cggcacgtga cttgtgcctg
    10621 cagagcatgg gcggcgtgtt ccacctcgcg accagccggc gcgccctgtt ctgctcgcag
    10681 ctggtgctgc agagctaccg ccaggccggc gtgccgctca ccgacgcgga tccgcgcctg
    10741 atcagcccgg ccgacatcct gcacatgcgc gaaggcgacg tgccgtcggt gcgggtcaag
    10801 cggccgctgc ggttcctcgg ccacctcaag tacgaggtgg cggcggccgt gccgctcgcg
    10861 cactaggcgc gcagcgcgtc gagcagttgc ggcatcacct tcggcgcggc ggcggcgatt
    10921 tcgccgctgt cgaggaatcc cgccttgccg tcgagatcgc tcaccaggcc gccggcctcc
    10981 tggatcagca gcgcgcccgc cgccatgtcc cagcgcgaca gccccatctc ccagaaggcg
    11041 tcgaggcgcc ccgccgcgac gtaggcgaga tcgagcgccg cggcgccggc gcgtcgcacg
    11101 cccgccgcgg tcgacatcac cgtgcgcagc tggcgcatgt aggcgtcgat gtggtcgagc
    11161 tccttgaacg gaaagcccgt gccgaccagc gcgcccgcca gctgcgtgca cttggaggcg
    11221 cgcatgcgcc ggtcgtcgag aaaggcgccg cgtccgcgcg aggcggtgaa cagctcgttc
    11281 ttcgccgggt cgtacaccac cgcctgcgtc aggacgccct tgtgctcgag cgcgatcgac
    11341 acgcagtact gcggaaagcc gtggatgtag ttggtggtgc cgtccagcgg gtcgatgacc
    11401 cacaggaact cgggccgtgc gcccgcgctc gcgcccgact cctcggcgag gaagccgtgc
    11461 tcggggtagg cgcggcggat gacgtcgatg atcgcctgct cggccgcctt gtccacctgc
    11521 gtgacgaaat cgttgcggtt cttggcgcgg acttcgagtc cgccttccag cgcggcgcgg
    11581 ttgatgagcg cgccggcgcg ccgcgcggct ttcaccgcga tgttgagcat cggatgcatg
    11641 tcctattcta gactgcgcgc atgtccgccc cgcgtatcgt gctgtgccgg ccgagccacc
    11701 ccggcaacat cggcgcggcg gcgcgcgcga tgaagaccat gggccttgcg gacctgcggc
    11761 tggtggcgcc ggcgcgcccg ccggcgccgc aggacgcatg gatggcgacg cacggcgcgg
    11821 acgtgctggc ggcggcgcaa acacacgcca cgctcggcga ggcgattgcg gactgcgcgg
    11881 cggcgtttgc gctgtcggcg cggccgcgcg aatggtcgcc gcaggtgctc gatgcgcgca
    11941 cggcggcggc gcgcgcggcg acgttcgccg agcccgtcgc gttcgtgttc ggcagcgagg
    12001 acgccgggct gaccaacgag gaactgttcg cctgccagtt cctcgtgcac atccccgcgg
    12061 accccgagag cagctcgctc aacctcgcgc aggcggtgca ggtggtgtgc tacgagctgc
    12121 gcatggcgca gggcgcggcg gtgccgccga tgcgcaccga aaagctggcg acggtggccg
    12181 atctcgaagg gctgtacgcg cacctcgagg aggtggcccg cgcctcgggc ttcatggcgc
    12241 cgggctccaa gctgcgcgag cgctggcggc ggctgttttc cagggtgccg gggctggagc
    12301 gcgaggaggt caacatcctg cgggggctac tcaagtccct gtacaagaaa tagttgatgg
    12361 atttactcgg ataccctaga gttcctgcat gttcaaacat ctacgcgaag acatcgcaag
    12421 catctttgcc cgcgatccgg cggcgcgcac gaccttcgag gtgctgacct gctacccggg
    12481 gctgcacgcc atctacatcc acaagctggc gcgctggttc tggacccacg gcttgcgctg
    12541 gcttgggcgc ttcacctcgc acgtggggcg ctgggccacc ggcatcgaaa tccaccccgg
    12601 cgtcatcatc gggcggcgcg tgttcatcga ccatggcatg ggcgtagtga ttggcgagac
    12661 ggcggaggtc ggcgacgact gcaccatcta ccaaggcgta acgctcggcg gcacctcgct
    12721 gtatcgcggc gccaagcgcc atccgacgct cggcgccggc gtggtggtcg gggcgggcgc
    12781 caaggtcctc ggcggtttca ccgtcggcgc gggcgcgaag atcggctcca atgccgtggt
    12841 ggtgacggcc gtgccggcag gggcgacggt ggtcggcatt ccgggccgca tcatcgagga
    12901 agccacggcg cagaaggcgc ctcactttgc cgcctacgcg gtggagcagg gtcaggatga
    12961 cccctttgca caggaaatcc agaagctggc agaacactcc gccgaactgg agcaggccct
    13021 ggccaagctc aaggaaaagc tcgccgatct ggaaaaagac cagcgcaggg atcagcttag
    13081 ggcggtcaag tgagttgttg actgtttctg tcgggaattg ctatacttga gcgcgttaat
    13141 caagtattcg aggcagacat gagactcacg accaagggca ggttcgcggt gaccgcgatg
    13201 gtggatctgg ccatgcgcca gtcgcgcgga cccgtggcgc tcgccgcgat cagcgagcgc
    13261 cagcacatct cgctgtccta cctggagcag ttgttcggca agctgcgccg gcgcaagctt
    13321 gtatccagcg tgcgcggtcc tggcggcggc tacaacctcg cgcagccggc cgccgatgtc
    13381 acggtcgccg acatcgtcac cgcggtggac gagccgctcg atgcgacgca gtgcggcggc
    13441 aaggagaact gcgccgacga aaagcgctgc atgacgcatg atctgtgggc cacgctcaac
    13501 agcaagatgt acgagtacct gtcctcggtg agcctcgccg acctggtcgc gcaccagggc
    13561 ggcaagcaca ttgccgtgct gcacgatcac cggcgcgcgc cggacaagga agaggtggcc
    13621 gcgtgaagct gccgatctac ctcgattact ccgcgaccac gccggtggat ccgcgcgtgg
    13681 cgcagaagat gatcccgtac ctcacggaga acttcggcaa tccggcaagc cgctcgcacg
    13741 ccttcggctg gaaggccgag gagggcgtgg aacaggcgcg cgcgcacgtc gcggcgctga
    13801 tcggcgccga tccgaaggag atcgtctgga cctcgggcgc caccgagggc aacaacctcg
    13861 ccatcaaggg cgcggcgaac ttctacaaga ccaagggcaa gcacctggtg acgcagaaga
    13921 ccgagcacaa ggcgacgctc gacacgatgc gcgagctcga gcgccagggc ttcgagtgca
    13981 cctatctcga cgtggaggaa aacggcatcg tcgatctgaa gaagctggaa gcggcgctgc
    14041 gccccgacac cattctcgtg tcgatcatga tggtcaacaa cgagatcggc gtgatccagc
    14101 cgatcgccga gatcggcgag ctgtgccgcg cgcgcggcat cattttccat tgcgacgcgg
    14161 tgcaggccgc gggcaagctt cccatcgacc tggggacgct gaaggtggac ctgctgacga
    14221 tcaccgcgca caagatgtac ggccccaagg gcatcggcgc gctttacgtg cgccgcaagc
    14281 cgcgggtgag gatcgaaccg cagatccacg gcggcggcca cgagcggggt tttcgctcgg
    14341 gcacgctcgc cacgcaccag atcgtcggct tcggcgaggc cgcgcgcctg gcgaagctcg
    14401 aaatggcgac cgacaacgag cgcatccgcg cgctgcgcga ccggctgtgg cgcggcctgt
    14461 cgtcgatgga ggcggtggtg ctgaacggcg acctggagcg gcgcattccc ggcaacctca
    14521 acgtgagctt caattacgtc gagggcgagt cgctgatcat ggccatcaag gacatcgcgg
    14581 tgtcctcggg ctcggcctgc acctcggcct cgctcgagcc ctcgtacgtg ctgcgcgccc
    14641 tggggcgctc ggacgagctg gcgcactcgt cgatccgctt cacgctcggc aagttcacca
    14701 ccgaggaaga ggtcgactac gcggtgaacc ttgtgcaggc caaggttgcc aaactcagag
    14761 agctgtcgcc gttgtgggag atgtacaagg acggcgttga cttgaatacg gtccagtggg
    14821 ccgcacattg aagtaggagc acgctatggc atacagcgac aaagtcatcg atcactacga
    14881 gaacccgagg aacgtgggct cgttcgacaa gggcgacgag gcggtgggca ccggcatggt
    14941 cggcgcgccg gcctgcggcg acgtgatgaa gctgcagatc cgcgtcggca aggacggcgt
    15001 catcgaggac gcgcgcttca agacctacgg ctgcggctcg gccatagcgt cgagctcgct
    15061 ggtgaccgaa tgggtgaagg gcaagcgcct ggaagaggcc gcggcgctga agaacacgca
    15121 gatcgccgag gagctgtcgc tgccgccggt gaagatccac tgctcgatcc tcgccgagga
    15181 cgcgatcaag gcggcgatcg ccgactatcg caagaagcac ggcctggaag cggcccaaga
    15241 gcagaaggaa aaggtggcct gacatggcag tgacgctgac agaaaaagcg gcgacgcacg
    15301 tgcaggacta cctcgccaag cgcggcaagg gcgtggcgct gcgcgtgggc gtgcgcacct
    15361 cgggctgctc cggcatggcg tacaagctgg agttcgccga cgcggtgaac ccggaggacc
    15421 accagttcga gagccacggc gtgaaattgg tggtcgaccc gaagagcctg ccgtttctcg
    15481 aaggcacgca gctcgactac acccgcgagg gcctgaacga ggggttcaag ttcctcaacc
    15541 ccaacgtgaa ggacgagtgc gggtgcggcg agtcgtttaa cgtctgaagg accatttcgc
    15601 cctcttcggg ctggcgccgg cattcgcggt cgacgccgcg gcgctggagg gcgcgtaccg
    15661 cgacatccag tcgcgcatcc accccgaccg ccatgcccat gccggtgacg ccgagcgccg
    15721 cgccgcgatg cagtggacca cgcgcgtgaa cgaggcctat cgcacgctga agagcccggt
    15781 gcagcgcgcc aggtacctgc tggagatgcg cggcgtcgac gtcgggctcg agaccaacac
    15841 gcagatgccg gccgagttcc tcacgcgcca gctcgagctg cgcgaggcgc tcgccgaagc
    15901 caccgggccg ggtgcgctcg atcgcctgca cgacacggtg cgcgcgcaaa cgcgcgacct
    15961 ggagcaggcg ctcgcggacc tgatcgacgt gcgccgcgac tatgccgcgg ccgccggcca
    16021 ggtgcgaaag ctcatgttcc tgcacaagct cgccgaggag atcgaccggg cctacgagga
    16081 gaacgaatag tggcactcct gcagatttcc gagcccgggc gctcgccggc gccgcaccag
    16141 cgccgtgtcg ccgtgggcat cgacctcggc accaccaact cgctggtggc ggcggtgagg
    16201 agcggcgtgc ccgtggtact tgcggacggg ctgggccggc cgctgctgcc ctcggtggtg
    16261 cgctaccaca gggacggacg cgtcgacgtc ggttacgcgg cccaggcgcg ccaggccgaa
    16321 gacccgcgca ataccgtcgt ctccgtgaag cgctacatgg ggcgcgggct gaaggacgtg
    16381 gtgcatgccg agaacgcgcc ttacgatttc gtcgacgcgc cgggcatggt gcagctgcgc
    16441 acggtggccg ggacgaagag cccggtagag gtgtcggcgg aaatcctgaa ggtcctgcgc
    16501 cagcgcgccg aattcgcgct cgccggcagt ctcgacggtg ccgtcatcac cgtgccagcc
    16561 tacttcgacg acgcgcagcg ccaggcgacc aaggacgccg ggcagctcgc cgggctgaac
    16621 gtgctgcggc tgctgaacga gcccacggcg gcggccatcg cctacggtct ggagaacgcg
    16681 gccgaaggcg tgtacgcggt ctacgacctc ggcggcggca cgttcgacat ctcgatcctg
    16741 cggctgtcga aaggggtgtt cgaggtgatg gcgaccgcgg gcgacgccgc gctcggcggc
    16801 gacgacttcg atcagcgcgt gttctgctgg gtggtggagg aggcgaagct cccgccgctc
    16861 ggcgcgaagg acatgcggct gctgctggtg aagtcgcggg aggcaaagga gtacctgacc
    16921 gcgcacgggg aggcgcgcgt cacggcgaag ctctccacgg gcgacgtggt cgacgtgtcg
    16981 ctcgacaccg aggccttctc cggcgtgacg cagcacctgg tgcagaagac gctcggcccg
    17041 atgcgccgcg cgctgcgcga cgcggggctg cggccggacc aagtgaaagg cgtggtgatg
    17101 gtcggcggtg cgacgcgcat gccgcagatc cagcgcgcgg tgggcgaatt cttccgctgc
    17161 gatcccctca acaacctcga tccggacaag gtggtggcga tcggcgccgc catgcaggcg
    17221 aacgtgctgg ccggcaacaa gccggcgggc gaggactggc tgctgctcga tgtgatcccg
    17281 ctgtcgctcg gggtcgagac catgggcggc ctcgccgaga agatcatccc ccgcaattcc
    17341 accattcccg tggcgcgcgc gcaggagttc accacctaca aggacggcca gaccggcatg
    17401 agcttccacg tcgtgcaggg cgagcgcgag ctggtggccg actgccggtc gctcgcgcgc
    17461 ttcgagctga agggcattcc gccgatggcg gcgggcgcgg cgcgcgtgcg cgtcacctac
    17521 caggtggatg ccgacgggct gctctcggtg caggcgcagg aagccaccac cggcgtcgag
    17581 gcctcggtgg cggtgaagcc ctcgtatggc ctggccgacg gcgagatcga gcgcatgctc
    17641 cgggattcct tcgagcatgc gcgggacgac atgcatgcgc gggcgctgca cgaggcgcgg
    17701 gtggacgcgg cgcggctgct cgaggcaacc cgctcggcgc tcgcggccga cggcgcgctg
    17761 ctcgagccgc acgagcgcgc cgagatcgag gcgcgcgtgg cggcgctgga cagcgcgctg
    17821 gcgggaagcg accatcgcgc gctgcgcgcc gggtccgacg cgctcaaccg ggcgaccgag
    17881 gagttcgcgg cgcgccgcat ggacgcaggg gtgcggcgcg cgctcgccgg gcgcaagatc
    17941 gccacgctgg acctgtagat ggcgcggatc aaggtgctgc cgcacgccga gctgtgcccg
    18001 cagggcgccg cattcgaggc gccggcgggc gtgtcgatct gcgatgcgct cctcgagcac
    18061 ggcgtggaca tcgagcacgc ctgcgagaag tcctgcgcct gcaccacctg ccacgtgatc
    18121 ctgcgccagg gcttcgattc gctcgacgcc gccgaggaca aggaggaaga tctgctcgac
    18181 aaggcctggg ggctggaggc cacctcgcgt ctgtcgtgcc aggccagggt ggcggacgcc
    18241 gacctgacgg tcgagatccc gaagtacacc atcaactacg agcgcgaagg cggcggcagg
    18301 aaatgaagtg gaccgacacc ctggatatcg cgatcgccct gagcgagaag taccccgagg
    18361 tcgacccgaa gacggtgcgc ttcaccgacc tgtaccagtg ggtgcagcag ctcgagggct
    18421 tcgacgacga tccgaagcgc tgcggcgaga agatcctgga aggcatccag atggcgtgga
    18481 tcgaggagtc gtagtgggcg tgctcggcga gcgcctggcg cgcgccttcg ccggacgccg
    18541 cgtgctggtg ctcggcggcg gcacgcagtg gagcgggtca ttctcgggca aggccgcgga
    18601 ggtgaccgtg caggagggtc tggacgcgct gccggatttc ggtcgccggc acgatgcgct
    18661 tttcgcgccc gaagtgtggt cgcggctgcc ctccgagcga ctggacgaat ttctgcggcg
    18721 agcggtacag gcggcggcgc cgggcgcgct gatcgccttc ctcgatcggc gggacgggac
    18781 gcccttggaa ggcgagctca tcggccgcgc ctcgcgacac ggctggggtg caaaccttga
    18841 gttgctgccg gattactggc tgctgacctg gtgggcgccg cgctgagcgc cccggtacgc
    18901 tacttcgcgc ggtatttgcc ccacagctgg tcgagccggg cgtagcggcc gcacccggtg
    18961 acatagaagc ggtaacgcgc cgggttcttc ttgtagtagt cctggtggta gtcctcggcg
    19021 ggccagaaat cgctcgccgc ctcgatcggc gtcacgatcg cgtccttgaa gggcttgtcc
    19081 ttctcccacc tggccttgga ggcctcggcg aggcgcttct gctcggcgtc gtggtggaag
    19141 atgcccggcc ggtactgcga gccgtggtcg cagaactggt gatcgagggc cgtgggatcg
    19201 tgattcagcc agaaggtctc gagcagcttg tcgtagctga cctgcttcgg gtcgtaggtg
    19261 acctgcagca cctccgtgtg gccgctgcgg cccgtggaca ccagcgcgta gctgggctcc
    19321 ttgatgtgcc cacccatgta ccccgagacc gtcgacagga cgcccggcac cttgtcgaag
    19381 gcctcctcgg tgcaccagaa gcagcccgcg gcgaaggtgg ccttggccag gccctgcgcc
    19441 tgcgcgagcg ccggcagcag cgtcgcgcac aacagcagca acaatcggtt catgacgaag
    19501 tcgcctcctg cccctttcgt cggggccggg ccggcatcct tactcgacct tcgccttcgc
    19561 gcgcagctcg cgcaccagct cctcgacctt ctgccgggcc aggcgctgct ggatctgcgg
    19621 gcgcacctgc gccagcggcg ggaagtccac cgcgcgcacg tcgtcgagct gaatcacgtg
    19681 gtagccgaag cgcgtgcgca ccggcgtgcc ggtgaacttg cccttctcca gcgccaccat
    19741 cgcgtcggcg aacggctttt ccagggtcga cgggatcttc cagtcgaggt cgccgcccag
    19801 ctcgcgcgtg ccctcgtcct tcgacgactt cttcgccagc tcatcgaact tgtcgccctt
    19861 ctgcaggtcc gcgatcaggc gcttcgcctc gtcctcggtc tcgacgagga tgtggcgcgc
    19921 cttgtactcg ttggcgccgg tctggtcgcg cacgcggtcg tattccttct tcacgtcggc
    19981 gtcggtgacc gcgttctcgc gcaggtaatc gctgaggtag gcgccgacca gcacttcctg
    20041 gcgcgcgacc tcgagctggg tctgcacttc ccgcttcttc gccgtgccgg cgcgcgccgc
    20101 cacctgcgac accagctcgc ggttgatgag gtcctcgcgc acgagggcgc gcgtctgctc
    20161 gttgtcgggg gcgccgcgct gcagctgctg gcgcatcagg tactcgaggc gcgcgcgcgg
    20221 gatggcgg
//
LOCUS       DBNHKE010000193        10007 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_879977, whole genome shotgun sequence.
ACCESSION   DBNHKE010000193 DBNHKE010000000
VERSION     DBNHKE010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_879977"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1917
                     /locus_tag="AC16O9_13235"
     CDS             <1..1917
                     /locus_tag="AC16O9_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6517615.1"
                     /translation="RPRMILLRTLSLARGPHTLLAGVSMTIERGWRLGLVGANGSGKT
                     SLLALLAGEIAPEAGDCEIQPGTRLARVEQEAPALARAVLEYLLDADGELRAAEDEIA
                     AAEARGDGEAIAHAHERLARADGYTARARAETLLAGLGFAPGDSARAVREFSGGFRMR
                     LNLARALVAPSDLLLLDEPTNHLDLDTILWLEGWLRAYAGTLIVVSHDRDFLDRVCDH
                     TLALLPAAAGGTARLYDGGYSQFEASRALELEQQFAAAARQDREIRRITTFVERFRAK
                     ATKARQAQSRLKRLERMARVAPVVAADTYRFEFEEPERQPERILALHGLDAGYPGRTV
                     LAGVELALTSADRVAVIGANGNGKTTLMRLLAGELAPLAGERIAARDIAIGYFAQHQV
                     ELLRADETPLGMMTRLYRGQREQALRDFLGRFGFAGERAERPIGPLSGGEKSRLALAA
                     LIKRRPNLLLLDEPTNHLDLEMRRSLAVALQSYSGALVIVSHDRSLLAACADRILVVA
                     DGRVRDFDGDLDDYRQLKLKEGRARDGEARPSRKDERRAAAASRAGLRQKLKPLEKRL
                     AAIEARLAQLAREIGEIRAALADPALYENADKERVAELARAESRLAEELAHAEEDWLH
                     ASARLEALRDEDFS"
     gene            complement(1910..2440)
                     /locus_tag="AC16O9_13240"
     CDS             complement(1910..2440)
                     /locus_tag="AC16O9_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517616.1"
                     /translation="MKLRQLKVDYNAEQDRLLMLIAASDAVELRMWLTRRFVKLLWPL
                     LVKLAEEASPRIRTQANPEARKALLGIEHQQAVQKADFSKAYEGKSRNMPLGEAPLLL
                     GRIQTGHDRKGQPVLAMHPAEGQGVTLTLDAVLLHSLCRLLQAAVKKSGWDMELKLPG
                     TDAAPAADEHAGRTLN"
     gene            complement(2556..3215)
                     /locus_tag="AC16O9_13245"
     CDS             complement(2556..3215)
                     /locus_tag="AC16O9_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR6517617.1"
                     /translation="MPAAPVASGAPAFEARILAPEAAARWAAAAPRPLVFTNGVFDIL
                     HRGHVTYLARARALGAALIVALNGDASARRLDKGPGRPVNPLADRLALVAALEAVDAV
                     TWFDDDTPAALIDACRPDVLVKGGDWPAAKIVGAAGVLARGGTVHSIPFEHARSTTAL
                     LERIGARGAARKTARRASAQRRKARAAGSRARGKGRSPKPSSAAYRGATRRTPRRAPR
                     R"
     gene            complement(3197..3805)
                     /locus_tag="AC16O9_13250"
     CDS             complement(3197..3805)
                     /locus_tag="AC16O9_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR6517618.1"
                     /translation="MKIVRRLTLAAAALAALLLLQGCVEMMVVGTGVAVATVEDRRTT
                     GVQLEDRGIELRAANRIDDRFGERVHVNVTSFNRYVLLTGEVGDEATRTEIETLTRGV
                     PNVLGVSNDLQIAGKSSTTSRSNDTLITSKVKTRFADANRFNVFHVKVVTEAAVVYLL
                     GIVTEQESADAVEIARTTAGVRKVVKMFEYCKPDDPVCRRRQ"
     gene            complement(3802..4419)
                     /locus_tag="AC16O9_13255"
     CDS             complement(3802..4419)
                     /locus_tag="AC16O9_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HIR6517619.1"
                     /translation="MGHAEQSAALAERVAAHFADSAQLKVAAAQALAGPIARAVEAMC
                     AALRGGGKVLACGNGGSAADAQHFAAELVNRFERERPPLAAVSLAADSSNLTSIANDY
                     GYEQVFEKQVRALGRKGDVLLAISTSGNSESVLRAMSAARELGLVTVALTGRGGGKMG
                     VALGAGDVHVCVPHERTMRIQEVHLLALHCLCDGIDSLLFGANKP"
     gene            complement(4458..4799)
                     /locus_tag="AC16O9_13260"
     CDS             complement(4458..4799)
                     /locus_tag="AC16O9_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6517620.1"
                     /translation="MNAAGMRAEELCAELLRKAGLRILARNWRCRLGEIDLVAEDGAT
                     LVFAEVRLRRDAAHGGAGESITAAKRARLLAAARLYLAGRAGTDCRFDVLLLDQLAPA
                     RIRWIRNAFGE"
     gene            4798..5667
                     /gene="rsmI"
                     /locus_tag="AC16O9_13265"
     CDS             4798..5667
                     /gene="rsmI"
                     /locus_tag="AC16O9_13265"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6517621.1"
                     /translation="MAPLTPPPTLPLTVPQGTLFVVATPIGNLADVAPRALQTLREAD
                     VVVCEDTRTTRTLLARHGIERATVALHAHNERAASAQVLADLQAGRSVALVSDAGTPA
                     VSDPGAWLVAAAHRAGVRVVPVPGPSAALAAYSAAGFAAGGFLFVGFLPPKTSARRKA
                     LAALDVPWPLVLYEAPHRVRECVQDLLERFGGEREVVIARELTKKFEEVARLPLAAAP
                     AWLEADAHRQQGEFVLVLGPGAPAAPDAEAAGRVLDALLEALPAGEAAKLAARITGVP
                     RNALYKRALERGK"
     gene            5677..6708
                     /gene="pyrC"
                     /locus_tag="AC16O9_13270"
     CDS             5677..6708
                     /gene="pyrC"
                     /locus_tag="AC16O9_13270"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR6517622.1"
                     /translation="MDRLTLIRPDDWHLHLRDGPQMAAVLPDSARRFARAIVMPNLRT
                     PVRTTQEALAYRERILAALPEGAQFEPLMTLYLTDNTPPEEIAAARASGQVHAVKLYP
                     AGATTHSTAGVTRISRCFHALEKMEALGMPLLVHGEVTDPEVDVFDREAVFIEEVLSP
                     LIERFRGLKVVLEHITTREAAQFVEVTGPNIAATITAHHLLLNRNALFLGGVRPHHYC
                     LPVLKREAHREALVEAAISGNPKYFLGTDSAPHARHTKEAACGCAGIYTAHAALELYA
                     AAFEEAGALDKLEGFASRFGPQFYGLPVNSGSVTLERREWTVPASLQFGAEQLVPLRA
                     GETVPWTLA"
     gene            6804..7442
                     /locus_tag="AC16O9_13275"
     CDS             6804..7442
                     /locus_tag="AC16O9_13275"
                     /inference="COORDINATES: protein motif:HMM:NF022673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HIR6517623.1"
                     /translation="MRTERGRPVRFVPPAASDPYYEVHLYETGQVQTRAGNRHDLFNA
                     LAWLAFPRTKARINALHAAAIPHEGAKRGRLRDLLTILDEGGVLVACAEPELKALVRE
                     FRWKDLFWERRERVRAAMRFIVLGHATLEQALAPWPGITCKALFIDPVLDADAQAADW
                     LARLPPDASPRLLAPLPVFGYPGWLPETGGADFYLDTRYFRPPGRGAAATEA"
     gene            7453..7786
                     /gene="rnpB"
                     /locus_tag="AC16O9_13280"
     ncRNA           7453..7786
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16O9_13280"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            7926..8873
                     /locus_tag="AC16O9_13285"
     CDS             7926..8873
                     /locus_tag="AC16O9_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517624.1"
                     /translation="MHDRLNQNDVTNNINVEDPEQVRDAVLTLFAARYPGADLSPLRG
                     AFEDVKSLFHGTFPGYLPCDTPYHDLRHTLDMTLAMARLLDGHDRSHAGDDRIGARRA
                     MLGMVVALLHDSGYMKRASEAHIENGAVFTKIHVSRSADFLSRYLPRIGFAEEAPAAA
                     KLVHFTGYEMDIDDIMLHDPKDRLLGYLVGTADLIGQMSDRMYLEKCREFLYPEFVWG
                     RIAREKLDDGREIVRYASPEDLMLKTPGFYEYVARARIEKKLGGADRYAEAHFDGPSL
                     YQSEIDRNMRFLKDAIEEADLKRLRRFCYSLSRRDAKVA"
     gene            8967..9413
                     /locus_tag="AC16O9_13290"
     CDS             8967..9413
                     /locus_tag="AC16O9_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR6517625.1"
                     /translation="MTVAERLRLGATRPEVAPALFRLLAEAALSRAALDACGLPVALA
                     DAAGSRPISYVNQAFEAFFGYRAAEALGRPAHVLLAMDAARAEQLYCEGAPGVVLSTQ
                     RKDCSLARVEVSVRAVRGSDGRVTHWVLAFSDRTELEQLRERLSRP"
     gene            complement(9400..9567)
                     /locus_tag="AC16O9_13295"
     CDS             complement(9400..9567)
                     /locus_tag="AC16O9_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517626.1"
                     /translation="MIFKIIAGIVAAALLIIFVGPVVVKLKDAALTVVVLIGLTMMLV
                     DLWQSLKSKDD"
     gene            complement(9583..>10007)
                     /locus_tag="AC16O9_13300"
     CDS             complement(9583..>10007)
                     /locus_tag="AC16O9_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6517627.1"
                     /translation="IMEAEMAANLDQEWERGRERISPNLRAQLERGREAKALDYQRAR
                     ARIPLLNEGFDEIFERCDAIVTPAAPGTAPAGLEATGDPAFCSLWTLCGMPALNLPLM
                     RGANGLPMGVQLVGRRDNDARLLRTARWLVAQVEAGGG"
BASE COUNT         1419 a         3659 c         3469 g         1460 t
ORIGIN      
        1 cgtccccgca tgattctcct gcgcactctc agcctcgccc gcggcccgca cacgctgctc
       61 gccggcgtct cgatgacgat cgagcgcggc tggcgcctgg ggctggtcgg cgcgaacggc
      121 agcggcaaga cgagcctgct cgcgctgctc gcgggcgaga tcgcgcccga ggccggggac
      181 tgcgagatcc agcccggcac gcgcctcgcc cgcgtcgagc aggaagcgcc ggcgctggcg
      241 cgcgccgtgc tcgagtacct gctcgatgcg gacggcgagc tgcgcgcggc cgaagacgaa
      301 atcgcggcgg ccgaggcgcg cggcgacggc gaggccatcg cgcacgcgca cgagcggctg
      361 gcgcgggccg acggctacac ggcgcgcgcg cgcgccgaaa ccctgctcgc cggcctgggc
      421 tttgccccgg gcgacagcgc gcgcgcggtg cgcgagttct cgggcggctt tcgcatgcgc
      481 ctgaacctcg cgcgcgcgct ggtcgcgccg tcggacctgc tgctcctcga cgagccgacc
      541 aatcacctcg acctggacac catcctgtgg ctggagggat ggttgcgcgc ctatgcaggc
      601 accctgatcg tcgtgtcgca cgaccgcgac ttcctcgatc gcgtctgcga ccacacgctg
      661 gcgctcctgc cggcggcggc gggcggcacg gcgcggctct acgacggcgg ctactcgcag
      721 tttgaggcga gccgcgccct ggagctggag cagcagttcg ccgcggcagc gcgccaggac
      781 cgcgagatcc gccgcatcac cacgttcgtc gagcgctttc gcgccaaggc gaccaaggcg
      841 cgccaggcgc agagccgtct gaagcgcctg gagcgcatgg cgcgcgtcgc gccggtggtc
      901 gccgcggaca cttatcgctt cgagttcgag gagcccgagc gccagcccga gcgcatcctg
      961 gcgctgcacg ggctcgacgc gggctacccg gggcgcaccg tcctcgcggg cgtggagctc
     1021 gcgctcacca gcgcagaccg cgtcgcggtg atcggcgcca acggcaacgg caagaccacc
     1081 ctgatgcgcc tgctcgcggg ggagctcgca cccctcgccg gcgagcgcat cgcggcgcgc
     1141 gacatcgcga tcggctattt cgcccagcac caggtcgagc tgctgcgcgc cgacgagacg
     1201 ccgctcggga tgatgacgcg cctctaccgc ggccagcgcg agcaggcgct gcgcgacttc
     1261 ctcggccgct tcggcttcgc gggtgagcgc gccgagcggc cgatcgggcc gctctcgggc
     1321 ggcgagaagt cacgcctcgc gctcgccgcg ctgatcaagc gccggccgaa cctgctgctg
     1381 ctcgacgagc cgaccaacca cctcgacctg gagatgcgac ggtccctggc cgtggcgctg
     1441 cagagctaca gcggcgcact ggtgatcgtg tcgcacgacc ggagcctcct tgccgcgtgc
     1501 gccgatcgca tcctggtggt cgcagacggg cgagtgcgcg atttcgacgg cgatctcgac
     1561 gactaccggc agctaaagct gaaggaaggc cgcgcgcgcg acggtgaggc gcgcccgtcg
     1621 cgcaaggacg agcggcgcgc cgccgccgcc tcccgcgccg gcctccggca gaagctgaag
     1681 ccgctggaga agcgcctcgc cgccatcgag gcgcgcctcg cgcaactcgc gcgcgagatc
     1741 ggcgagatcc gcgccgcgct cgccgacccg gcgctctacg agaacgcgga caaggaacgc
     1801 gtggccgagc tggcgcgcgc cgagtcgcgc ctcgccgagg agctcgcgca cgccgaggag
     1861 gactggctgc acgcctctgc gcgcctggaa gccttgcgcg acgaagactt tagttgagcg
     1921 tgcgcccggc gtgctcgtcg gccgcgggcg ccgcgtcggt gcccgggagc ttgagctcca
     1981 tgtcccagcc gcttttcttc accgccgcct gcagcagccg gcacagcgaa tgcagcagca
     2041 ccgcgtccag cgtcagcgtg acgccctgcc cctcggccgg atgcatggcg agcaccggct
     2101 gacccttgcg gtcgtgcccg gtctggatgc gtccgagcag gagcggcgct tcgccgagcg
     2161 gcatgttgcg cgactttcct tcgtaggcct tgctgaagtc ggccttctgc actgcctgct
     2221 ggtgctcgat gccgagcagc gccttgcgcg cctcgggatt ggcctgggtg cggatgcgcg
     2281 ggctcgcctc ctcggcgagc ttcacgagca gcggccacag cagcttcacg aagcggcgcg
     2341 tgagccacat gcgcagctcc acggcgtcgc tcgccgcgat cagcatgagc agccgatcct
     2401 gctcggcgtt gtagtccacc ttgagctggc gcagtttcac cggagcatga tagcaatgct
     2461 gtgcctggtc ggtgccgggg gcggcggcgg ggcggcccag atgaagcggc tctacgtgcc
     2521 cctagcgttg cagggccgca gcctcgcaca ggcaattatc gccgcggcgc gcgcctcggg
     2581 gtacgccgtg ttgcgcctcg atacgctgcc gaagatggtt tcggcgatcg ccctttaccg
     2641 cgcgctcggc ttcctgccgc gcgcgccttg cgccgctgcg ccgacgcccg gcgcgctgtc
     2701 tttcgggctg cgcccctggc cccgatccgc tcgagcagcg ccgtcgtcga gcgcgcgtgc
     2761 tcgaacggga tcgaatgcac cgtgccgccg cgtgcaagca ccccggccgc gcccacgatc
     2821 ttcgccgccg gccaatcgcc ccccttcacc agcacgtcgg ggcggcaggc gtcgatgagc
     2881 gcggcaggcg tgtcgtcgtc gaaccaggtg accgcatcca ccgcctcgag cgccgcgacc
     2941 agtgccaggc ggtcggcgag gggattgacc ggccggcccg gccccttgtc cagccgccgc
     3001 gccgaagcgt cgccgttcag cgccacgatg agcgccgcgc cgagcgcgcg tgcgcgtgcg
     3061 aggtaggtga cgtgcccgcg gtgcaggatg tcgaacacgc cgttggtgaa cacgagcggc
     3121 cgcggcgctg ccgctgccca gcgcgccgcg gcctccggcg cgaggatcct ggcctcgaag
     3181 gccggcgcgc cggacgctac tggcgccgcc ggcacactgg gtcgtcgggc ttgcagtact
     3241 cgaacatctt caccaccttg cgcacccccg ccgtggtgcg cgcgatctcc accgcgtcgg
     3301 ccgactcctg ctcggtgacg atgccaagca ggtacaccac cgcggcctcg gtcaccacct
     3361 tgacgtgaaa cacgttgaaa cggttggcgt cggcgaagcg cgtcttcacc ttggaggtga
     3421 tgagggtgtc gttggagcgc gaggtggtcg aggacttgcc ggcgatctgc aggtcgttgc
     3481 tgacgcccag cacgttgggc acgccgcgcg tcagggtctc gatctcggtg cgggtcgctt
     3541 cgtcccctac ttccccggtg agcaggacgt agcggttgaa cgaggtcacg tttacgtgca
     3601 cgcgctcgcc gaagcggtcg tcgatccggt tcgccgcgcg cagctcgatg ccgcggtcct
     3661 cgagctgtac gccggtggtg cgccggtcct cgaccgtcgc cacggccacg ccggtaccca
     3721 cgaccatcat ctcgacgcac ccctgcagca ggagcagggc cgcgagcgcc gccgcggcaa
     3781 gagtcaatct gcgaacgatt ttcatggctt gttggctccg aacagcaggg aatcgatgcc
     3841 gtcgcacagg cagtgcagcg cgaggaggtg cacctcctgg atgcgcatgg tgcgctcgtg
     3901 cggcacgcac acgtgcacgt cgccggcacc gagcgcgacg cccatcttgc cgccgccgcg
     3961 gccggtcagc gccaccgtca ccaggccgag ctcgcgcgcc gcggacatcg cgcgcagcac
     4021 gctctccgag ttgcccgagg tcgagatcgc cagcagcaca tcgcccttgc ggcctagcgc
     4081 ccgcacctgc ttctcgaaca cctgctcgta gccgtagtcg ttggcgatcg aggtgaggtt
     4141 ggagctgtcg gccgccagcg acaccgcggc gagcggcgga cgctcgcgct cgaagcggtt
     4201 gacgagctct gcggcgaaat gctgcgcgtc ggcggccgag ccgccgttgc cgcaggcgag
     4261 caccttgccg ccgccgcgca gcgccgcgca cattgcctcg acggcgcgcg cgatcggtcc
     4321 ggctagcgcc tgcgctgctg cgactttcag ttgcgcgctg tcggcgaagt gcgccgcgac
     4381 gcgctcagcg agcgccgcgc tttgctcggc gtgacccatg gcggcatgat tctacacgcc
     4441 gcgccaacgc ctcgcgccta ctcgccgaag gcgttgcgga tccagcggat gcgcgccggc
     4501 gccagctggt cgagcagcag cacatcgaag cggcagtcgg tgccggcgcg cccggcgaga
     4561 tacaggcgcg cggcggcgag cagccgcgcg cgcttggccg cggtgatgct ttcgccggcg
     4621 ccgccatggg cggcgtcccg gcgcaggcgc acctcggcga agaccagcgt cgcgccgtcc
     4681 tcggccacca ggtcgatctc gcccagccgg cagcgccagt tgcgcgccag gatgcgcagt
     4741 cccgctttgc gcagcagctc ggcgcaaagc tcctcggctc gcatgcccgc cgcgttcatg
     4801 gcacccttaa cgccaccgcc caccctcccg ttgaccgtgc ctcagggaac cctgttcgtc
     4861 gtcgccacgc ccatcggcaa cctcgccgac gtcgcgccgc gcgcgctcca gaccctgcgc
     4921 gaggcggatg tcgtggtgtg cgaggacacg cgcaccacgc gcacgctgct cgcgcgccac
     4981 ggcatcgagc gcgcgaccgt cgccctgcac gcgcacaacg agcgtgccgc ctcggcacag
     5041 gtgctcgccg acctgcaggc cggccgcagt gtcgccctgg tgagcgacgc cggcacgccc
     5101 gcggtctcgg acccgggcgc atggctggtc gcggcggcgc accgcgccgg cgtgcgggtc
     5161 gtccccgtcc ctggcccgag cgccgccctg gcggcctact cggccgccgg ctttgcggcg
     5221 ggcgggttcc tgttcgtcgg cttcctgccg ccgaagacct cggcgcggcg caaggcgctg
     5281 gcggcgcttg acgtgccgtg gccgctcgtc ctgtacgagg cgccccatcg cgtgcgcgag
     5341 tgcgtgcagg acctgctcga gcgcttcggc ggcgagcgcg aggtcgtcat tgcgcgcgag
     5401 ctgacgaaga agttcgagga ggtggcgcgc ctgccgctcg ccgcggcgcc cgcctggctc
     5461 gaggccgatg cgcaccgcca gcagggcgaa ttcgtgctgg tcctcggccc cggcgcgcct
     5521 gccgcgcccg acgccgaggc cgctgggcgc gtgctcgacg cgctgctcga ggcgctgccc
     5581 gcgggcgagg cggcgaagct cgcggcgcgc atcaccggcg tgccgcgcaa cgcgctctac
     5641 aagcgcgcgc tggaacgcgg caagtagaat cgcgccatgg atcgcctcac gctgattcgt
     5701 cccgacgact ggcacctgca cctgcgcgac ggcccgcaga tggccgcggt gcttcccgac
     5761 agcgcgcggc gcttcgcgcg cgccatcgtc atgcccaacc tgcgcacgcc ggtgcgcacc
     5821 acgcaggagg cgctcgccta tcgcgagcgc atcctggcgg cgctgccgga aggcgcgcag
     5881 ttcgagccgc tgatgacgct ttacctcacc gacaatacgc cgccggagga gattgcggcc
     5941 gcgcgcgctt cgggccaggt gcacgcggtg aagctttacc cggccggcgc gaccacgcac
     6001 tccacggcgg gcgtgacgcg catctcgcgc tgctttcacg cgctggagaa gatggaagcg
     6061 ctggggatgc cgctgctcgt gcatggcgag gtcaccgacc ccgaggtgga cgtgttcgat
     6121 cgcgaggcgg tgttcatcga ggaagtactc agcccgttga tcgagcgttt ccgcggtctc
     6181 aaggtggtgc tcgagcacat caccacgcgc gaggccgcgc agttcgtgga agtcaccggc
     6241 cccaacattg ccgccaccat caccgcgcac cacctgctgc tcaaccgcaa cgccctgttc
     6301 ctgggcggcg tgcggccaca ccactactgc ctgcccgtgc tcaaacgcga ggcgcaccgc
     6361 gaagcgctgg tcgaggccgc catctcgggc aatcccaagt acttcctcgg caccgacagc
     6421 gcgccgcatg cgcgccacac gaaggaagcg gcgtgcggct gcgccggcat ctatacggcg
     6481 cacgccgcgc tcgagctcta tgcggcggcg ttcgaggaag ccggggcgct ggacaagctc
     6541 gaagggttcg ccagccgttt cggcccgcag ttctacggcc tgccggtcaa cagcggcagc
     6601 gtcacgctgg agcgccgtga gtggacggtg ccggcgtccc tccagttcgg cgccgagcag
     6661 ctcgtgccgc tgcgcgcggg cgagaccgtg ccctggacgc tcgcctagcc catccgatct
     6721 tcgatccgct gcggccctgg ctcgaggcgc tgggccccgc gcccggactc gacgcgctca
     6781 acgcggccgc cgagcgcgcc gcgttgcgca ccgagcgcgg gcggccggtg cgcttcgtgc
     6841 cgccggcggc aagcgacccg tactacgagg tccacctgta cgagaccggg caggtacaga
     6901 cgcgcgcggg caaccgccac gacctgttca acgcgctcgc ctggctcgcc tttccccgca
     6961 ccaaggcgcg catcaacgcg ctgcacgccg cggcgatccc gcacgagggc gcaaagcgcg
     7021 ggcgcctgcg tgacctgctg accatcctgg acgagggcgg cgttctggtg gcgtgcgccg
     7081 aacctgagct caaggccttg gtacgcgagt tccgctggaa ggacctgttc tgggagcgtc
     7141 gcgaacgggt gcgcgcggcc atgcgcttca tcgtgctcgg gcacgcgacg ctcgagcagg
     7201 cgctcgcgcc ctggcccggg atcacctgca aggcactgtt catcgacccg gtgctcgacg
     7261 ccgacgcgca ggccgcggac tggctcgcgc ggctgccgcc ggacgcatcg ccgcgcctgc
     7321 tcgcgccgct gccggtattc gggtatcccg gctggctgcc cgagaccgga ggtgccgatt
     7381 tctacctcga cacgcgctac tttcggccgc cggggcgcgg ggcggcggcc accgaggcgt
     7441 agaatgcgca tgggagatgg ccaggcagcc gctgtggcga cacagaggaa agtccgggct
     7501 ccacagggcg ggatgcgggg taactccccg gcgcgcataa gcgctgcgca agcggcgtca
     7561 aggcgacgaa cagggccaca gagacgagtc ctcggggtca cacccgaggg gtgaaacggg
     7621 caacctccat ccggagcaac accaaatagg caagcgcctg aggccggccc ggccgagctt
     7681 gcgggtaggt ggcttgagcc cgcgggtgac cgcgggccca gaggaatggc tgccgcgagg
     7741 ggttcgcccc ttggcacaga acccggctta tcggccgtct cccattttca ccgccgcgtt
     7801 cgcgcgctct ttgtgcaccg cagttgacaa ttccttttgc cttcggcgac gcgacggcgg
     7861 gataatgcgg gaactcgatc cgtgccggag gaagaacggg ccgccaaggc ccgcacaact
     7921 aaaaagtgca cgaccgtctc aatcagaacg acgtcaccaa caacatcaac gtagaggacc
     7981 ccgagcaggt ccgcgacgcg gtgctcaccc tgttcgcggc acgctacccc ggggccgacc
     8041 tgtcgccctt gcgaggcgcc ttcgaggacg tcaagtccct gttccacggg acttttcccg
     8101 ggtacctgcc ctgcgacact ccgtatcacg atctgcgcca cacgctcgac atgacgctgg
     8161 cgatggcgcg cctgctcgac gggcacgacc gcagccacgc cggggacgat cgcatcggcg
     8221 cgcgccgcgc catgctcggc atggtggtcg cgctgctgca cgattccggc tacatgaagc
     8281 gcgcctccga ggcgcacatc gagaacggcg cggtgttcac caagatccac gtcagccgca
     8341 gcgccgactt cctctcgcgc tacctgccga gaatcggctt tgccgaggag gcgcccgccg
     8401 cggccaagct ggtgcatttc accggctacg agatggacat cgacgacatc atgctgcacg
     8461 accccaagga ccgcctgctc ggctacctgg tgggcacggc cgacctgatc gggcagatgt
     8521 cggaccgcat gtacctggag aaatgccgtg agttcctcta tccggagttc gtctggggcc
     8581 gcatcgcacg cgagaagctc gacgacggac gcgagatcgt gcgctacgcc tcgcccgaag
     8641 acctgatgct gaaaacgccc gggttctacg agtacgtcgc ccgcgcgcgc atcgagaaga
     8701 agcttggcgg cgccgaccgc tacgccgagg cgcacttcga cggcccgagc ctgtaccaga
     8761 gcgagatcga ccgcaacatg cgcttcctga aggacgccat cgaggaagcc gacctgaagc
     8821 gcctgcggcg cttctgctat tcgctgtcgc gccgggacgc caaggtcgct tagcgggcgt
     8881 acgtactgtg gggtttgtca cgattccccg catttagccg ccccggcgct tgcgctcgcg
     8941 ccgcggctcg tggcaaatag aattccatga cggttgctga aagattgcgc ctgggggcca
     9001 cgcgcccgga ggtcgcgccc gcgctgttcc ggctgctcgc cgaggcggcg ttgtcgcgcg
     9061 cggcgctgga tgcctgcggc ctgccggtgg cgcttgccga cgcggccggc tcgcggccga
     9121 tctcctacgt gaaccaggcg ttcgaggctt tcttcggcta ccgcgccgcg gaggcgctcg
     9181 ggcgcccggc gcacgtgctg ctggcgatgg atgcggcgcg cgccgagcag ctctactgcg
     9241 agggcgcgcc tggcgtcgtg ctgagcacgc agcgcaagga ctgctcgctg gcgcgggtcg
     9301 aggtttcggt gagggcggtg cgcggcagcg acggccgcgt cacgcactgg gtgctggcgt
     9361 tctccgaccg caccgagctc gaacagctgc gcgagcggct cagtcgtcct tagacttcag
     9421 cgactgccag aggtcgacca gcatcatcgt cagcccgatc agcaccacca cggtgagcgc
     9481 ggcgtccttg agcttgacga ccaccggccc gacgaaaatg atgagcaggg ccgccgccac
     9541 gatgcctgcg attatcttga atatcattgc ccgattctcc gtttagccgc cgcccgcttc
     9601 gacctgcgcc acgagccagc gcgccgtgcg cagcagccgc gcatcgttgt cgcgccgtcc
     9661 caccaactgt acccccatcg gcagtccgtt cgcgccgcgc atcagcggca ggttgagtgc
     9721 cggcatgccg cacagcgtcc acaggctgca aaacgccgga tcgcccgtcg cctccaggcc
     9781 tgccggcgcc gtgcccggcg ccgccggcgt gacgatggcg tcgcagcgct cgaatatctc
     9841 gtcgaagcct tcgttcagca gcggaatgcg cgcgcgcgcg cgctggtagt cgagcgcctt
     9901 cgcctcgcgc ccgcgctcca actgggcgcg caggttcggc gagatgcgct cgcgcccccg
     9961 ttcccattcc tggtcgaggt tggcggccat ctcggcctcc atgatct
//
LOCUS       DBNHKE010000194        15454 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_140672, whole genome shotgun sequence.
ACCESSION   DBNHKE010000194 DBNHKE010000000
VERSION     DBNHKE010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_140672"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC16O9_13305"
     CDS             complement(<1..403)
                     /locus_tag="AC16O9_13305"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF047080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR6517628.1"
                     /translation="MQSAKAITFAHCAASYIESHRAGWRNAKHAEQWTNTLQNYAEPV
                     IGPLPVQDVDTGSVLRILEPIWAKKPETASRLRGRIENILDWAKARGYRSGENPARWK
                     GHLNQLLPTLSKKDRVIHHKAMPFLEIGAFIL"
     gene            complement(431..781)
                     /locus_tag="AC16O9_13310"
                     /pseudo
     CDS             complement(431..781)
                     /locus_tag="AC16O9_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024750.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            864..1682
                     /locus_tag="AC16O9_13315"
     CDS             864..1682
                     /locus_tag="AC16O9_13315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517629.1"
                     /translation="MRTDLQGRTVLLTGAAGGIGRVLTKAFLECGANVIAADVDAVGL
                     KRLQRSAKLVTRILDIADPAACEAAVRGAQIDILINNGALGMHVLRETCMTDLVRIEE
                     ITPEFWNRMISVNLSGAWYLTRAAVPGMLARRWGRIINVTTSFFTMLRGRFHPYGPAK
                     AGLEAMSAGHANEFDGSGVTVNVVVPGGPADTPMVPAASGLRRADLVPPAKMAPPMLW
                     LASREADGVTGKRYIAVKWDEALPAGAARRASESPIGWPDLAGAPVWPGGKPPA"
     gene            1700..2131
                     /locus_tag="AC16O9_13320"
     CDS             1700..2131
                     /locus_tag="AC16O9_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6517630.1"
                     /translation="MSQSKPLSGKRWNFVHNLASAAKWTPGLREIFEYRDLGLKDGTK
                     GDYVAHLIRHNGKKVKDQVQQWHAHDCTFQFVYVLNGSATFEYEGQGRRTIRKGDMIL
                     QTPGIAHREIECSEDFEVLEIVAPADFATRTVPAPKEAAAA"
     gene            2373..2693
                     /locus_tag="AC16O9_13325"
     CDS             2373..2693
                     /locus_tag="AC16O9_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517631.1"
                     /translation="MNALQHSTEFIRLESDLAARLEALFQRCPALHGFSVQPASSVTR
                     ERIVVDLQDDLCLADVVWHQALNGEQAVVLVREISQELYDLVDERPEAAALLRGRTFA
                     SVLH"
     gene            2704..3285
                     /locus_tag="AC16O9_13330"
     CDS             2704..3285
                     /locus_tag="AC16O9_13330"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6517632.1"
                     /translation="MLRHLLVGIALILGPGVSEAQDLAKPLLLVAKPELKGPYNHTAL
                     LVVPVDGRHIGFILNRATEVKLAALFPDHAPSAKVADPVYFGGPERVNAIFAIVARNP
                     GAPSVRLFGDLHVTAQAEVVDRIIEPTPNDARYFAGFVGWRPGELAAELESGFWYTAE
                     PEAALVFSDDTGAMWESLIRRLGKPMDEGMHAA"
     gene            3369..4157
                     /locus_tag="AC16O9_13335"
     CDS             3369..4157
                     /locus_tag="AC16O9_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6517633.1"
                     /translation="MKYDTLQFDVQDQVAWITLHRPAALNALNLQMTKELYDVANRCG
                     DASVRAAVLTGGGERAFCAGGDVADFAAAGEGLEVLIKDMTGHLHMAISRFAWMRAPL
                     IGAINGVAAGAGFSLALCCDLAIAADNAKFASAYSKIGFTPDGSSTYFLSRLLGRRRA
                     MELYLTERVLSAQEALDWGLVNRVVPGKDLQAEAKKLAQQIARGPTLAYGGVKKLLQM
                     APLEGLESQMERETRFIVQAGTSADGREGARAFVEKRAPRFTGT"
     gene            4210..6528
                     /locus_tag="AC16O9_13340"
     CDS             4210..6528
                     /locus_tag="AC16O9_13340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6517634.1"
                     /translation="MNAPEIQFDPAAARFGAARSQKRLEDDRLLVGKGLFSDDRRFEN
                     EAALVVVRSPHAHARIKSVDLAAGRAAPGVLAVWTLAELAADGVKHIPVSPLFKRPGG
                     QPMSAPPRTPLAGERALYVGQGVVAIVAQTRVQAQDAAELVEIDYEDLPCVVDPRQAV
                     KPGAPQLWPDAPGNIAGETRYGKADEAAAAFKRAAHVTEIELHNQRVIAVAMEPRCAI
                     GVHEKGRTTVYTQSQQPTGARDALAAALGVKPADLRVVNGDIGGGFGMKTGATPEEAL
                     VAYAARKLGRPVRWRGDRSEEFLAAHMGRDMHFNAALALDKDGRILALKLHMLGNMGA
                     VPVASSATIPLAMVARVQTSVYRVPAADFHFQGVLTNTMATGAYRGAGRPEGNFLIER
                     LVEKAGREMGIDSVEIRKRNLLQPGDLPHTTYTGEVYDSGDFPKILDGLLKVADWKGY
                     GARAEQSKKRGKLRGRGLSAYLEWTGALPTETVDIEVDADGKVTVFSGTQAMGQGLET
                     TYVQLITEVLQIPAAAVTIVQGDTDRANGVGSVGSRSAFVGGSAVVAAGRTLIVRGKE
                     LAADALETAGADIEFRDGRFVVAGTDKSVGLAELAARQADRRVRVSATQTPSTPSWPN
                     GAQACEVEIDPDTGEVAIARLSSLDDIGRIINHAIVEGQIQGGMAQGVGQALHEQSVY
                     DPESGQLLTGSLMDYCVPRADQFPPMTNAFDESVPCTTNLLGVKGCGEIGTIGAVPAV
                     VHAVLDALAGFGVTHLEMPLTPEKVWRAMQRR"
     gene            6536..7237
                     /locus_tag="AC16O9_13345"
                     /pseudo
     CDS             6536..7237
                     /locus_tag="AC16O9_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
     gene            complement(7258..8325)
                     /locus_tag="AC16O9_13350"
     CDS             complement(7258..8325)
                     /locus_tag="AC16O9_13350"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517635.1"
                     /translation="MDLLEIDGLHQAYNGTEVVRGLDFSLARGDIGCLLGPSGCGKTT
                     VLRCIAGFEPLRAGRILLNGEVVSGPGVMLAPEHRQIGMVFQDYALFPHLTVSENIAF
                     GLRDAPRVLRAARVRELAALTGLAGALRQYPHELSGGQQQRVALARALAPRPELLLLD
                     EPFSNLDVDLRERLSLEVREIIKANGATAILVTHDQHEAFAMADRIGVMHEGRIEQCD
                     SAYNLYHQPANRFVADFVGQGVFLPAKVLNASSVEMELGRLEGEIPGACQIGCDACRR
                     GCLTDILLRPDDVVHDDAAPIKAEVVHKAFRGAEILYTLKLASGRRVLALVPSHHNHA
                     LGEHIGIRLDVDHVVVFQPER"
     gene            8456..10018
                     /locus_tag="AC16O9_13355"
     CDS             8456..10018
                     /locus_tag="AC16O9_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6517636.1"
                     /translation="MLWVAASLAQPASDVWRHIASTLLGAYVANTAILVALVACGVLS
                     IGVLSAWLIASYRFPGARVLEWALVLPLAMPAYVMAYAYTDWLQFTGPVQGALRALTG
                     WQAREYWFPEIRSLGGAALVLALALYPYVYLIARTAFREMPRSSLEAGRLAGLSAWGT
                     FFGVALPLARPAIAAGTALALMETLADFGTVSYFALEVFTTGIFKAWLAMGDRVAAAQ
                     LSSCLLGFVVLVIALERASRGRAAFHATAPAKPVPPQLLRGGAAALATLACALPVVLG
                     FLVPAAILVELAASEPQLRWSARLVGLVGNSFTVSGVTAVAAVLLALVMAYAARLSRS
                     RVALTANRVAALGYAMPGAVIAIGVLVPLAQLDNWLAGLVESVTGAPSGLLLTGSIAA
                     LVYALLVRFLAVALSTTEAGLARITPSMEDAARSLGASPAATLARVHAPLVARSLATA
                     GLLVFVDVMKELPATFALRPFNFDTLAVEAYHLAKDERLAEAAVPSLVIVAVGLVPLV
                     ALSRRYFQATRS"
     gene            complement(9994..11016)
                     /locus_tag="AC16O9_13360"
     CDS             complement(9994..11016)
                     /locus_tag="AC16O9_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6517637.1"
                     /translation="MPVIKALIYIFGAALAFPALAQDKVLNIYSSRHYQTDEALYAGF
                     TQRTGIKINRIEAGEDALIERIRNEGARSPADVLITVDAGRLWRAEQLGLFQPVKSAL
                     LEKRIPASFREPGGHWYGFSMRARVIAYAKDRVKAAEVPTYEALAAPRWKGRLCVRSS
                     TNIYNLSLMGALIEQLGEVQAEAWAKAVRANMARDPKGGDTDQLKAVAAGECDVAISN
                     HYYYARLARSAKPEDQALVAKLGIVFPNQSGRGTHVNVSGAGVLKHAPHPAAAVQFLE
                     YLAGDDAQRYFADGNNEWPVVPSVRVDNPVLAALGEFRYDTLNVAALGRNQPASQKIY
                     DRVAWK"
     gene            complement(11101..11493)
                     /locus_tag="AC16O9_13365"
     CDS             complement(11101..11493)
                     /locus_tag="AC16O9_13365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR6517638.1"
                     /translation="MSEERAEALLQTLLALGVDYRYGGGSPESGFDCSGLVAHVFREA
                     YGIELPHNARAQSEFGVRVSLSELRAGDLVFYNTLNRPYSHVGIYLGDGRFVHAPKTG
                     AQVRVESINGRYWMRRFDGARRIQPTRL"
     gene            11615..12307
                     /locus_tag="AC16O9_13370"
     CDS             11615..12307
                     /locus_tag="AC16O9_13370"
                     /inference="COORDINATES: protein motif:HMM:NF015891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR6517639.1"
                     /translation="MKATTYISLLLACFAAWGQASLEVIDLRHTTAEQVLPALRPLLE
                     PGGVLTGQRSQLIIRTSPANLAELRRALQTLDAPARRLAILVRFAGTSADQRSELAVG
                     VRGSRIEARARQERSTGAERVDQRIQVLEGRRARIATGTSRPLELRDGTLIQDVATGF
                     DVIPRLAGDTVLLEIAPQREVAGRVTRGSVQVERASSTVRARLGEWVELAGVDDAGTS
                     RRIWVRVDEVRP"
     gene            12229..14238
                     /gene="yoaA"
                     /locus_tag="AC16O9_13375"
     CDS             12229..14238
                     /gene="yoaA"
                     /locus_tag="AC16O9_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase YoaA"
                     /protein_id="HIR6517640.1"
                     /translation="MGGARRGRRRRDEPAHLGAGGRGPALSSAASQALARLFAADGPL
                     AAHIPGYRVRAQQLELARAILEAIERDAPFVAEAGTGTGKTLAYLVPALLAGGKVIVS
                     TGTKTLQDQLHDRDLPAVRNALASGAVTALLKGRANYVCRHRLERAALEARLGSREEA
                     VQLQRIERFAAGSASGDRAELPDVPEDAPVWQHATSTRENCLGQECPRYDDCFVMKAR
                     RGALAADVVVVNHHLFFADVVLRDEGVAELLPACNTIILDEAHQLPDTARLFFGEILA
                     SGRLAELARDARLELAAAGGAAPELERPAARLEKAARDLRLAFPEEPARMAWSQAAAR
                     PAFAGALAELGEALAGLAAALAAQAERSEGLKSCAQRSGEARDLLGRLSGEQDGKRVR
                     WVEVFPHAVQLHLTPLSCAELFQRQLEEAPRAWIFTSATLAVGGDFSHYQREMGLQGA
                     AARSWPSPYDFARQALLYVPPALPEDPNDPGFTEAVVNAALPVIEASRGRAFLLFTTL
                     RALRRAHQLLRERLPYPLLVQGTGSRSELLARFRRLGNAVLLGSASFREGVDVRGEAL
                     SVVVIDKLPFAPPDDPVLAARIDALRAAGGNPFTEFQLPQAILQLKQGAGRLIRDETD
                     RGVLMVCDPRLYSRPYGRLVRASLPPMKPTRELAEVRAFLARIGA"
     gene            14235..15200
                     /gene="denD"
                     /locus_tag="AC16O9_13380"
     CDS             14235..15200
                     /gene="denD"
                     /locus_tag="AC16O9_13380"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HIR6517641.1"
                     /translation="MKILITGGGGFLGFRLARALLARGNLGGKPITSLTLLDGAFPEA
                     AAKEPRLKAVTGDVSDPKVIAAVCPPDTDAVFHLAAVVSGAAEADFDLGMRVNLQGTE
                     LLLAQMRKCAKPPRLVYTSSVAAFGGALPAVLDDSTIANPQTSYGTQKVIGEYLVSDY
                     SRKGFLDGRSLRLPTIVVRAGKPNAAASSFASGILREPLNGVASECPVAPETGVWMLS
                     PGRVVEAFLHAWELPASAWGAQRWLNLPGITASVAQMIEALKKVAGPKVAGLVSYKPD
                     ARIQAIVKTWPANFKTPRALAMGFTPDADVESIIRDYIADENLKL"
     gene            complement(15201..>15454)
                     /gene="bamD"
                     /locus_tag="AC16O9_13385"
     CDS             complement(15201..>15454)
                     /gene="bamD"
                     /locus_tag="AC16O9_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR6517642.1"
                     /translation="YYMKRGAFVAAANRAQAAVADYPQAPAVEEALFIMLQAYRALGV
                     EDLRADAERVMRRNFPDSPYFKGPVKRDVSWWRIWDPDW"
BASE COUNT         2289 a         5346 c         5425 g         2394 t
ORIGIN      
        1 tgagaatgaa ggcacctatc tcaagaaagg gcatcgcctt gtggtgaatt acgcggtcct
       61 tcttggagag cgtgggaagc aactgattga ggtggccctt ccaacgcgcc gggttttcgc
      121 cggatcggta gcccctggct ttggcccagt ccagaatatt ctcaattcgt cccctgagcc
      181 tgctggcggt ctccggcttc ttggcccaaa tgggctcaag gatcctcaga actgacccgg
      241 tatcgacgtc ttgaacgggc agggggccaa ttacgggctc ggcgtagttc tgaagcgtat
      301 tcgtccactg ctcggcatgt ttagcattac gccaccctgc cctatgggac tcgatgtagg
      361 acgctgcgca atgcgcgaaa gtaatggcct ttgccgactg caaggtcctg cgacggtctt
      421 cttgctcccg cgcctcgatg gggtccaggc ggtctagaag tacccgacgt tgtgccgcag
      481 ccttctcccg ggcctccggt agccctacag agtggaccgc gcccaagccc atatctcgca
      541 gcctctgcgt gaggggagac cgaaaccgaa atatccacgc cttgctaccc gaggacgtga
      601 cctggagata tagcccacca ccgtcgcagt acaggccagg gtgtttcttg gttgctaccg
      661 tcctggctga aagccgattg agttgtcgcg tacccatact cctacccata tattgaaggc
      721 ggatttcaat gaaccgtctt acactagctt ggagtggcat tccgccgttc tcatgccaca
      781 tcaaacggtt accagtcaaa caatggacgt tgttggactg tgctggatag aaactttagg
      841 gactccctct ccgccatttc gccatgcgca cggacctgca aggtcgcact gtccttctga
      901 ccggagcggc cggcggcatc ggtcgcgtgc tcacgaaggc gttcctcgag tgcggcgcga
      961 atgtcatcgc ggccgacgtc gacgccgtcg gcctcaagcg gctgcagcgc tccgccaagc
     1021 tcgtcacccg catcctcgac atcgcggacc ctgcggcttg cgaggcggcg gtgcgtggcg
     1081 cgcagatcga catcctgatc aacaacggcg cgctcggcat gcacgtcctg cgcgagacct
     1141 gcatgaccga cctcgtgcgg atcgaggaga tcacacccga attctggaac cggatgatca
     1201 gcgtgaacct ctcgggcgcg tggtacctga cgcgcgccgc agtcccgggc atgctcgcgc
     1261 gccgctgggg tcgcatcatc aacgtcacca ccagcttctt caccatgctg cgcgggcgat
     1321 ttcatccgta cggtccggcc aaggcggggc tggaggcgat gtcggccggg cacgcgaacg
     1381 agttcgacgg cagcggggtc acggtgaacg tcgtggtgcc cggcgggccc gccgatactc
     1441 cgatggtgcc cgcggcctcg gggctgcggc gcgcggacct ggtgccgccc gcgaagatgg
     1501 cgccgccgat gctgtggctc gcctcgcggg aggcagacgg cgtcacggga aagcgctaca
     1561 ttgcggtcaa gtgggacgag gcgcttcccg ccggggcggc ccggcgggcg tcggagtcgc
     1621 cgatcggctg gcccgatctc gccggcgcgc ccgtgtggcc gggcggcaag ccgcctgcat
     1681 gaccaacagg agaacgacga tgagtcaaag caagcccctg agcggcaagc gctggaactt
     1741 cgtgcacaac ctggcgagcg cggccaagtg gacgccggga ttgcgcgaga tcttcgagta
     1801 ccgcgacctc gggctgaagg acggcacgaa gggcgattac gtcgcccacc tcatccgcca
     1861 caacggcaag aaggtgaagg accaggtgca gcaatggcac gcgcatgact gcaccttcca
     1921 gttcgtctac gtgctgaatg gctcggccac cttcgagtac gagggccagg gccggcgcac
     1981 gatccgcaag ggcgacatga tcctgcagac gcccggcatc gcgcaccgcg agatcgagtg
     2041 ctccgaggac ttcgaggttc tggagatcgt cgcgcccgcc gacttcgcca cgcgcacggt
     2101 gccggcgccg aaagaggctg ccgcagcgta agacgcctca gccggggtgg gaggcgacct
     2161 cccgcccagg gtgcctgaac gcactgccgc cgcccccgcc ctgggcctag ggtggggtga
     2221 tgagacgcgg agcgaccccc agccccatcg gccgcgcgtc cggcctagcg ccccgttcgt
     2281 cgccgcgggg cgtcatggac tgtggcgggc gggactcctt cctcgacccc acggcccccg
     2341 gcgcgcgatg ctgggggcgc aggaggtggc atatgaacgc gctacagcac agtaccgagt
     2401 tcatccggct ggagtccgac ctggccgcgc gcctggaggc gctgttccag cgctgcccgg
     2461 cgttgcacgg cttttcagtg cagccggcct cgagcgtcac ccgcgagcgc attgtggtcg
     2521 atctgcagga tgacctctgc ctcgccgacg tcgtttggca ccaggcgctg aacggcgagc
     2581 aggccgtcgt cctggtgcgc gaaatctcgc aagagctcta cgacctcgtg gacgagcgcc
     2641 cggaagccgc ggcgctgctg cgcgggcgca ccttcgccag tgttctgcat tgagggggct
     2701 cacgtgctga gacacctgct cgttggcatc gctctgatct tggggcccgg cgtctcggag
     2761 gctcaggatc tcgccaagcc gctcctgctg gtggccaagc ccgagctcaa gggcccgtac
     2821 aaccacaccg cgctgctcgt cgtcccggtc gacggccggc acatcggctt catcctgaac
     2881 cgcgccaccg aagtcaaact cgccgcgctg tttcccgatc atgcgccctc ggccaaggtc
     2941 gccgaccccg tgtacttcgg cggccccgag cgcgtcaacg cgatctttgc catcgtggcg
     3001 cgcaacccgg gcgcgccttc ggtgcgcctg ttcggcgacc tgcacgtcac cgcgcaggcc
     3061 gaggtggtcg accgcatcat cgagccgacg cccaacgatg cgcgctactt cgccggcttc
     3121 gtcggctggc gcccgggcga gctcgccgcc gagctcgagt cggggttctg gtacaccgcc
     3181 gagccggagg cggccctggt cttcagcgac gacactggcg ccatgtggga gtccctgatt
     3241 cggcgcctgg gcaagcccat ggacgagggc atgcacgcgg cgtagcaagg cccggccgtc
     3301 cgccacgtag gtcagaatac gcccctcgcg gggcgtattt ttttgcccgg cgcgacaagg
     3361 agcccggcat gaaatacgac accctgcagt tcgacgtcca ggaccaggtc gcctggatca
     3421 cgctccaccg gcccgccgcg ctcaacgcgc tcaacctgca gatgacgaag gagctgtacg
     3481 acgtcgccaa ccgctgcggc gacgcttcgg tgcgcgccgc ggtgctcacg ggcggcggcg
     3541 agcgcgcctt ttgcgccggc ggcgacgtcg ccgatttcgc cgccgcgggc gagggcctcg
     3601 aagtcctcat taaggacatg accgggcacc tgcacatggc gatctcgcgc ttcgcgtgga
     3661 tgcgcgcacc gctcatcggc gcgatcaatg gcgtcgccgc gggcgcgggc ttcagcctcg
     3721 cgctgtgctg cgacctggcg atcgcggcgg acaatgcgaa attcgccagc gcctactcga
     3781 agatcggctt cacgcccgac ggcagctcga cctacttcct gtcgcgcctg ctcgggcgcc
     3841 gtcgcgcgat ggagctctac ctgaccgagc gcgtgctgag cgcgcaggaa gcgctcgact
     3901 ggggcctggt caaccgcgta gtgccgggca aggacctgca ggccgaggcg aagaagctcg
     3961 cgcagcagat cgcccgcggg ccgacgctcg cctacggcgg cgtgaagaag ctgctgcaga
     4021 tggcaccgct cgagggcctc gaatcgcaga tggagcgcga gacgcgcttc atcgtgcagg
     4081 ccggcaccag cgccgacggg cgcgaagggg cgcgtgcgtt cgtggagaag cgcgcgccgc
     4141 gcttcaccgg cacctgatat attcaagacg ccccctcgcg ggtcgttttg ctttcccgac
     4201 cacgagccca tgaacgcacc cgaaatccag ttcgaccccg ctgccgcgcg cttcggcgcc
     4261 gcgcgctcgc aaaagcgcct cgaagacgac cggctgctcg tcggcaaggg cctgttcagc
     4321 gacgaccgtc gctttgagaa cgaagccgcg ctggtggttg tgcgctcacc ccacgcgcac
     4381 gcgaggatca agtccgtgga cctcgccgcg gggcgcgccg cgcctggcgt gctcgccgtg
     4441 tggacgctgg cggagctcgc cgccgacggc gtgaagcaca tcccggtgtc gccgcttttc
     4501 aagcgccccg gcggccagcc gatgtccgcg ccgccgcgca cgccgctcgc aggcgagcgc
     4561 gcgctctacg ttggccaagg cgtggtggcg atcgtcgcgc agacgcgcgt gcaggcgcag
     4621 gacgccgcag agctcgtcga gatcgactac gaggatctgc cgtgcgtggt cgatccacgg
     4681 caagcggtga agccgggtgc gccgcagctc tggcccgacg cgccgggaaa catcgccggc
     4741 gagacgcgct acggcaaagc cgacgaagcg gccgcagcgt tcaagcgcgc cgcgcacgtc
     4801 accgagatcg agctgcacaa ccagcgcgtg atcgcggtgg cgatggagcc gcgctgcgcg
     4861 atcggcgtgc acgagaaggg ccgcaccacg gtctacacgc agagccagca gcccaccggc
     4921 gcacgcgatg cgctcgccgc cgcgctcggc gtgaagccgg cggacctgcg cgtggtgaac
     4981 ggcgacatcg gcggcggctt cggcatgaag accggcgcaa cgccggagga agcgctcgtc
     5041 gcctacgcgg cgaggaagct cggccggccg gtgcgctggc gcggcgacag gagcgaagag
     5101 ttcctcgccg cgcacatggg ccgcgacatg catttcaacg cggcgctcgc gctcgacaag
     5161 gacgggcgca tcctcgcgct caagctgcac atgctcggca acatgggcgc ggtgccggtg
     5221 gcctcctcgg ccacaatccc gctcgccatg gtggccaggg tgcagacctc ggtgtaccgc
     5281 gtgccggcgg cggacttcca cttccagggc gtgctcacca acaccatggc gaccggcgcc
     5341 taccggggcg cgggccggcc cgagggcaac tttctcatcg agcggctggt ggagaaggcc
     5401 gggcgcgaga tgggcatcga ctcggtcgag atccgcaagc gcaacctgct gcagccgggc
     5461 gatctgccgc acaccacgta cacgggcgag gtctacgact cgggcgactt tccgaagatt
     5521 ctcgacgggc tactcaaggt ggccgactgg aagggctacg gcgcgcgcgc cgagcaatcg
     5581 aagaaacgcg gcaagctgcg cgggcgcggg ctctcggcgt acctggagtg gaccggggcg
     5641 ctgcccaccg agacagtcga catcgaggtg gacgccgacg gcaaggtcac cgtgttctcc
     5701 ggtacgcagg cgatgggaca ggggctggag accacctacg tacagctcat caccgaggtg
     5761 ctgcagattc ccgccgccgc ggtgacgatc gtgcagggcg ataccgatcg cgccaacggc
     5821 gtcggcagcg tcggctcgcg ctcggcgttc gttggcggct cggcggtggt ggcggccggg
     5881 cgcacgctca tcgtgcgcgg caaggagctc gccgccgacg cgctggagac ggcgggcgcc
     5941 gacatcgagt tccgcgacgg gcgcttcgtc gtcgccggca ccgacaagtc cgtgggcctg
     6001 gccgagctcg ccgcgcgcca ggcggacagg cgcgtgcgcg tctcggcgac gcagacgccc
     6061 tcgacgccgt cctggccgaa cggagcgcag gcctgcgagg tggagatcga tccggacacg
     6121 ggcgaagtgg cgatcgcgcg cctgtcgagc ctcgacgaca tcggtcgcat catcaaccac
     6181 gccatcgtcg aagggcagat ccagggcggc atggcacagg gcgtagggca ggcgctccat
     6241 gagcagtcgg tgtacgaccc ggaatccggg cagctgctca cgggttccct catggactac
     6301 tgcgtgccgc gcgccgacca gttcccgccc atgaccaacg ccttcgacga gagcgtgccc
     6361 tgcaccacca acctgctcgg cgtgaagggc tgtggcgaga tcggcaccat cggcgcggtg
     6421 cccgccgtgg tgcacgcggt gctcgatgcg ctcgccggct tcggcgtcac gcacctggag
     6481 atgccgctca cgccggaaaa ggtgtggcgc gcgatgcagc gccgctgagg acgcgatgcg
     6541 cgagcgcgcg cgccgcgttt tcggttacgc cgtcctcgcg tcgctcgtgc tgcacgccct
     6601 ggtgttctac gtggtgccgg cggcgcgcga gttcgccgcg ctggtgccgc cggagcccga
     6661 gccgctgatc gcgcgcgtcg agcagctcga gccgccgccg gccccagcac ccgcgcccgc
     6721 ggccaggccg aacccgaaac ccaagcccgc ggcgcagccc gaagcgccgc agcccgtacc
     6781 gtcggtgccg gtgccggtgc cggtgccgag ggccgagccc gtgcctgtcc cggcacctgc
     6841 agtggcgccg gtggccgtgc ccgcgccgcc ggcgcccgag cctgcgtcag aacctgtcgt
     6901 gcaggcgccg gtaccggcac cggcgccgct cgcgcgtgcc gcgccgcagc cgccgtctcc
     6961 ggatgcgggc gcgatcacgc gctttcgcca aggcattctg cgcaccgccc gggcggacaa
     7021 gcgctatccg cgcgtcgccg tcgacaacct ctgggagggc aaggtcgggg tgcgcatgtc
     7081 gatcggcgcc gacgggcgca tcgccgcgct gcgcgtgacg cgtggctcag ggcacgcggt
     7141 gctcgatcgc gaagcgctgc ggttgttcga gacggcccag tcgcgggtgc cgatcccgcg
     7201 cgagctgcgc gggcaggcgt tcgagatcga cctcgaggcg gtgtacgacc tgaccgatca
     7261 gcgctcgggc tggaacacca ccacgtggtc gacgtcgagg cggatgccga tatgctcgcc
     7321 gagcgcatgg ttgtggtggc tcggcaccag cgccagcacg cgccggccgc tcgcaagctt
     7381 gagcgtgtag aggatctccg cgccgcggaa tgccttgtgc acgacctcgg ccttgatcgg
     7441 cgcggcgtcg tcgtgcacca cgtcgtccgg tcgcagcagg atgtcggtga ggcagccgcg
     7501 gcggcaggcg tcgcagccga tctggcaggc gccagggatc tcgccttcga gccggccgag
     7561 ctccatctcc acgctgctcg cgttcagcac cttcgccggc aggaacacgc cctggccgac
     7621 gaagtcggcg acgaagcggt tcgccggctg gtgatacagg ttgtacgccg agtcgcactg
     7681 ctcgatgcgg ccctcgtgca tgacgccgat ccggtcggcc atggcgaagg cctcgtgctg
     7741 atcgtgcgtg accaggatgg cggtggcgcc gttcgccttg atgatctcgc gcacttccag
     7801 cgacaggcgc tcgcgcaggt cgacgtcgag gttggagaac ggctcgtcga gcaacagcag
     7861 ctcggggcgc ggcgccagcg cgcgcgccag cgccacgcgc tgctgctggc cgccggagag
     7921 ctcgtgcggg tactggcgca gcgcgccggc gaggccggtg agcgcggcga gctcgcgtac
     7981 gcgcgccgcg cgcagcacgc gtggcgcatc gcgcaggccg aaggcgatgt tctcgctcac
     8041 cgtcaggtgc gggaacagcg catagtcctg gaacaccatg ccgatctgcc ggtgctcggg
     8101 ggcgagcatc acgccgggtc ccgacaccac ctcgccgttg agcaggatgc gcccggcacg
     8161 cagcggctcg aagccggcaa tgcagcgcag gaccgtggtc ttgccgcagc cgctggggcc
     8221 cagcaggcag ccgatgtcgc cgcgggcgag cgaaaaatcg agcccgcgca cgacctcggt
     8281 accgttgtag gcctggtgca ggccgtcgat ttcgagcagg tccatgcgtc tcctattata
     8341 gagccgcaat gaacaataat cgttcttgtt agaatgtcga gtgcttttct ttcaatcatt
     8401 taggcacggt gcgggggttc tctccgtcgc cattgcggcg ctgctcgcgc tccccgtgct
     8461 ctgggtcgcg gcgagcctcg cgcagcccgc gagcgacgtc tggcgccata tcgccagcac
     8521 gctgctgggc gcatacgtgg cgaacaccgc gatcctggtc gccttggtgg cgtgcggcgt
     8581 gctgtcgatc ggcgtgctat cggcctggct catcgccagc taccgctttc caggcgcgcg
     8641 cgtgctggag tgggcgctgg tgctgccgct ggcaatgccg gcctacgtga tggcctacgc
     8701 gtacaccgac tggcttcagt tcaccggccc ggtgcagggt gccttgcgcg cgctcaccgg
     8761 ctggcaggcg cgcgagtact ggtttcccga gatccgctcg ctcggcggcg cagcgctggt
     8821 gctcgcgctt gcgctctacc cctacgtgta tctcatcgcg cgcaccgcgt tccgcgagat
     8881 gccgaggagc agcctcgagg ccgggcggct cgccgggctc tcggcgtggg gcacgttctt
     8941 cggcgtggcg ctgccgctcg cgcgcccggc gatcgcggcc ggcaccgcgc tcgcgctgat
     9001 ggagacgctg gccgatttcg gcaccgtgtc gtacttcgcg ctggaggtct ttaccaccgg
     9061 catcttcaag gcctggctgg cgatgggcga ccgcgtcgcc gcagcgcagc tctcgagctg
     9121 cctgctcggc ttcgtcgtgc tggtgatcgc gctggagcgc gcctctcgcg ggcgcgccgc
     9181 gttccacgcc acggcaccgg ccaagcccgt gccgccgcag ctgctgcgcg gcggcgccgc
     9241 ggcgctcgcc acgctcgcct gcgccctgcc ggtggtgctc ggctttctcg tgcccgccgc
     9301 gattctcgtc gagctcgcgg cgtccgagcc gcaactgcgc tggagcgcgc gcttggtcgg
     9361 cctggttggc aacagcttca cggtgtcggg ggtgaccgcg gttgccgccg tgctgctcgc
     9421 cctggtgatg gcctacgccg cgcggctgtc gcgtagccgc gtggcgctga cggctaaccg
     9481 cgtcgccgcg ctgggctacg ccatgcccgg ggcggtgatc gccatcggcg tgctggtgcc
     9541 gctcgcgcag ctggataact ggctcgcggg gctggtggag agcgtcaccg gggcgccgag
     9601 cgggctgctg cttaccggca gcatcgcggc tctggtctat gccttgctgg tgcgcttcct
     9661 cgccgtcgcg ctttcgacca ccgaagccgg cctcgcgcgc atcacgccga gcatggaaga
     9721 cgcggcgagg agcctcggcg cctcgcccgc ggcgacgctg gcgcgcgtgc acgcgccact
     9781 ggtggcgcgc agcctcgcca ccgcggggct gctggtgttc gtcgacgtga tgaaggagct
     9841 gcccgcgact ttcgcgctgc gtccgttcaa cttcgacacg ctggcggtcg aggcctacca
     9901 cctggcgaag gacgagcgcc tggccgaggc cgccgtgccc tcgctggtga tcgtggcggt
     9961 cggcctggtg ccgctcgtcg cgctctcgcg gcgctacttc caggcgacgc ggtcgtagat
    10021 cttctgcgag gcgggctggt tgcgcccgag cgcggccacg ttcagcgtgt cgtagcggaa
    10081 ctcgcccagc gcggcgagca ccgggttgtc cacgcgcacg ctcggtacca ccggccactc
    10141 gttgttgccg tcggcgaagt agcgctgcgc gtcgtcgccc gccaggtact cgaggaactg
    10201 caccgccgct gcggggtgcg gcgcgtgctt cagcaccccg gcacccgaga cgttgacgtg
    10261 ggtgccgcgg cccgactggt tcggaaacac gatgccaagc ttcgccacca gcgcctggtc
    10321 ctcgggcttc gccgagcgcg cgaggcgcgc atagtagtag tggttggaga tcgctacgtc
    10381 gcactcgccc gcggccaccg ccttcagctg gtcggtgtcg ccgcccttcg ggtcgcgcgc
    10441 catgttggcg cgcacggcct tggcccaagc ctcggcctgc acctcgccga gctgctcgat
    10501 cagcgcgccc atcagcgaca ggttgtagat gttggtggag gagcgtacgc acaggcgccc
    10561 tttccaccgc ggcgccgcga gcgcctcgta ggtgggcact tcggcggcct tcacccggtc
    10621 cttggcgtag gcgatgacgc gtgcgcgcat ggagaagccg taccagtggc cgccgggctc
    10681 gcggaaactc gccgggatgc gcttttccag cagcgcggac ttgaccggct ggaacagccc
    10741 gagctgctcg gcgcgccaca ggcgcccggc gtccacggtg atcagcacgt cggcggggct
    10801 gcgcgcgcct tcgttgcgaa tgcgctcgat gagcgcgtct tcgcccgcct cgatgcggtt
    10861 gatcttgatt ccggtacgct gcgtgaaacc cgcgtagagc gcctcgtcgg tctggtaatg
    10921 ccgcgacgaa tagatattca ggaccttgtc ttgggccaga gcaggaaacg ccaaagcggc
    10981 gccgaagata tatattagtg ctttaataac aggcatttga gcaccccatg ggaattgtgc
    11041 tcaaatgata accactctca tttatagagt caatagagat gggaacagtt cttggaaaga
    11101 ttacaggcgg gttggctgga tgcgccgggc accgtcaaag cgccgcatcc agtagcggcc
    11161 attgatcgat tcgacgcgca cctgtgcccc ggtcttcggc gcgtgcacga agcgcccgtc
    11221 accgaggtag atgccgacgt gcgagtaggg gcggttcagc gtgttgtaga acacgagatc
    11281 cccggcacgc agttccgata gactgacgcg cactccgaac tcgctctgcg cgcgggcgtt
    11341 gtgcggcagc tcgatgccgt aggcctcgcg gaatacatgc gccaccaggc cgctgcaatc
    11401 gaagcccgac tcgggcgatc cgccaccgta gcggtagtcc acgccgagcg cgagcagggt
    11461 ttgcagcagc gcctcggcac gctcctcgct cattggcccg gtgtccggcg gtggcagctg
    11521 ccgtggcacg gagccgcacc cgaaaatgga cgcaaacaac aataaataca agtatttgcg
    11581 aagcatacct aaagtaatct agaccattta ggtcatgaaa gcaaccactt acatttcgct
    11641 gctcctcgcc tgcttcgcgg cctgggggca ggcgagcctg gaggtgatcg acctgcgcca
    11701 caccaccgcg gagcaggtgt tgccggcatt gcggccgctg ctcgagcccg gcggcgtgct
    11761 cactggccag cgcagccagc tcatcatccg gacgagtccc gccaacctgg cagagctgcg
    11821 ccgcgccctg cagacgctcg atgcgcccgc ccgccggctg gcgatcctgg tgcggtttgc
    11881 gggcacctcc gcggaccagc gttccgagct cgcagtgggc gtacgcggca gccgcatcga
    11941 agcgcgcgcg cgccaggagc gatccacggg cgccgagcgc gtggaccagc gcatccaggt
    12001 gctggaaggg cgccgcgcgc gcatcgccac cggcacgagc cggccactcg agttgcggga
    12061 cggcacgctg atccaggacg tcgccaccgg gttcgatgtc atcccgcgcc tggccggcga
    12121 cacggtgctg ctcgagatcg cgccgcagcg cgaagtcgcg ggcagggtga cgcgcggcag
    12181 cgtgcaggtc gagcgcgcct cgagcacggt ccgcgcgcgc cttggcgaat gggtggagct
    12241 cgccggggtc gacgacgccg ggacgagccg gcgcatctgg gtgcgggtgg acgaggtccg
    12301 gccctgagct cggcggcgtc ccaggcgctg gcgcggctgt tcgccgcgga cgggccgctc
    12361 gcggcgcaca tcccgggcta ccgcgtgcgc gcgcagcagc tcgaactggc gcgcgcgatc
    12421 ctggaggcga tcgagcgcga tgcgccgttc gtcgccgagg ccggcaccgg caccggcaag
    12481 acgctcgcct acctcgtgcc ggcgctcctc gccgggggca aggtgatcgt ctccacgggc
    12541 acgaagacgc tgcaggacca gctgcacgat cgcgatctgc ccgcggtgag gaacgcgctc
    12601 gcctcgggcg cggtcaccgc gctgctcaag ggccgcgcca actacgtctg ccggcaccgc
    12661 ctggagcgcg cggcgctcga ggcgcgcctc ggcagccgcg aggaggcggt gcagctgcag
    12721 cgcatcgagc gcttcgctgc gggaagcgcg agcggcgatc gcgccgagct gcccgacgtg
    12781 cccgaggatg cgccggtgtg gcagcacgca acctcgacgc gcgagaactg cctcggccag
    12841 gaatgcccgc gctacgacga ttgcttcgtg atgaaggcgc gccgcggcgc gctcgccgcc
    12901 gacgtggtgg tggtgaacca ccacctgttc ttcgccgacg tcgtgctgcg cgacgagggc
    12961 gtggctgaac tgctgccggc gtgcaacacc atcatcctcg acgaggcgca ccagctgccg
    13021 gacaccgcgc ggcttttctt cggcgagatc ctggcgagcg gtcgcctggc ggagctcgcg
    13081 cgcgacgcgc gcctggagct cgccgccgcg ggcggcgcgg cgcccgagct cgagcgcccc
    13141 gccgcgcgcc tggagaaagc ggcgcgcgac ctgcggctcg cgtttcccga ggagcccgcg
    13201 cgcatggcgt ggtcgcaggc cgcggcgcgg ccggcgttcg ccggcgcgct ggccgagctg
    13261 ggcgaggcgc tggccgggct cgcggcggcg ctcgcggcac aggccgaaag gagcgaaggc
    13321 ctcaagtcct gcgcccagcg ttcgggtgag gcgcgcgatc tcctcgggcg gctctctggc
    13381 gagcaggatg gcaagcgcgt acgctgggtg gaggtgttcc cccatgcggt acagctgcac
    13441 ctgacgccgc tttcctgcgc agagctgttc cagcgccagc tcgaagaggc gccgcgcgcc
    13501 tggatcttca cctcggcgac gctcgccgtg ggcggcgact tctcgcacta ccagcgcgag
    13561 atggggctgc agggggcggc agcgcgcagc tggccgagcc cctacgattt cgcgcgccag
    13621 gcgctcctct acgtaccgcc ggcgctgccc gaggacccca acgatcccgg gttcaccgag
    13681 gcggtggtga acgccgcgct gccggtgatc gaagcaagcc gcgggcgcgc gttcctgctc
    13741 ttcaccacgc tgcgtgcgct gcgccgcgcg caccagctgc tgcgcgaaag actgccgtat
    13801 ccgcttctcg tgcagggcac cggctcgagg agcgagctgc tggcgcgatt ccggcgcctt
    13861 gggaacgcgg tgctgctcgg cagcgcgtcg tttcgcgagg gcgtggacgt gcgcggcgaa
    13921 gcgctttccg tggtggtgat cgacaagctg ccgttcgccc cgcccgacga cccggtgctc
    13981 gcggcgagaa tcgacgcgct gcgcgccgcg ggcggcaacc cgttcaccga gttccagctg
    14041 ccgcaggcga tcctgcagct caagcagggc gccgggcggc tgatccgcga cgagaccgac
    14101 cgcggcgtgc tcatggtgtg cgacccgcgg ctgtactcgc gcccctacgg gcgcctggtg
    14161 cgcgccagcc tgccgccgat gaaaccgacg cgcgagcttg cggaagtgcg cgcgtttctc
    14221 gctagaatcg gcgcatgaag atcctcatca ccggtggtgg cggctttctc ggcttcaggc
    14281 tcgcccgggc gctgctcgcg cgcggcaatc tcggcggcaa gccgatcacg tccctcacct
    14341 tgctcgatgg cgcgtttccg gaggccgcgg cgaaggagcc gcggctgaag gccgtgaccg
    14401 gcgatgtctc cgatccgaag gtgatcgccg cggtgtgccc cccggacacc gacgccgtgt
    14461 ttcatctcgc ggccgtggtg agcggcgcgg ccgaggccga cttcgacctc ggcatgcgcg
    14521 tgaacctgca gggcaccgag ctgctgctgg cgcagatgcg caagtgcgcg aagccgccgc
    14581 gcctagtcta taccagctcg gtggcagcct tcggcggcgc gctgcccgcg gtgctggacg
    14641 actcgacgat cgccaacccg cagacctcgt acggcacgca gaaggtgatc ggcgaatacc
    14701 tggtgagcga ctattcgcga aagggcttcc tcgatggccg gtcgctcagg ctgccgacga
    14761 tcgtggtgcg cgcgggcaag cccaacgccg ccgcgtcgtc gtttgcgagc ggcatcctgc
    14821 gcgagccgct gaacggcgtc gcctcggaat gcccggtggc gcccgagacc ggcgtgtgga
    14881 tgctgtcgcc cgggcgcgtg gtggaggcgt tcctgcacgc gtgggagctg ccggcctcgg
    14941 cctggggcgc gcagcgctgg ctgaacctgc cgggcatcac ggcgagcgtt gcgcagatga
    15001 tcgaggcgct gaagaaggtg gcgggcccca aggtcgccgg cctggtgtcc tacaagcccg
    15061 acgcgcgcat ccaggccatc gtgaagacct ggccggcgaa cttcaaaacg ccgcgcgcgc
    15121 tggcgatggg ctttacgccg gacgccgacg tcgagtcgat catccgcgac tacatcgccg
    15181 acgagaacct caagctctga ttaccagtcc gggtcccaga tgcgccacca ggaaacgtcg
    15241 cgcttcaccg ggcccttgaa gtaggggctg tcggggaaat tcctgcgcat gacgcgctcg
    15301 gcgtcggcgc gcagatcctc gacgccgagc gccctgtagg cctgcagcat gatgaacagc
    15361 gcctcctcca ccgccggcgc ctgcggatag tccgccaccg cggcctgtgc acggttggcg
    15421 gcggcgacaa aggcgccgcg cttcatgtag tagc
//
LOCUS       DBNHKE010000195         3234 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1039918, whole genome shotgun sequence.
ACCESSION   DBNHKE010000195 DBNHKE010000000
VERSION     DBNHKE010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1039918"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC16O9_13390"
     CDS             complement(<1..451)
                     /locus_tag="AC16O9_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6517643.1"
                     /translation="MRPESPDRVAARAAVRELTASKIRELFNAGIGRKDVVPFWVGEP
                     DEPTPEFIRKAGIASIEAGELFYSSNYGIAELREALAAYLTRLHRTTAPGEVVVTSAG
                     VNALMLVSQLLVDPGDRVVEVVPLWPNLQEIPKILNARVATVALEFSP"
     gene            479..1408
                     /locus_tag="AC16O9_13395"
     CDS             479..1408
                     /locus_tag="AC16O9_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6517644.1"
                     /translation="MAVDTASLQKWALAAGCALMVTWGVNFAITKVVLAELGVWPFLF
                     IRFLAMPLAGFGLLVTITRRHFPRTWPRREDLARFAAAGLIGHTAHVSLVMWGINLST
                     AFSSSLVLTSSPLWTLLILALLGAEKLHGRQLAGTLLAFAGIVVFLSDKFVRGFALAG
                     AGDLVLLAAASLFSLYTVLTKPLVERYGPLNVMCYSLLFGAPPLVLATLPAFLDAPLA
                     GISAGVWFGVFWAVAVSSFLGWIVWAWVNSVRGLARSAPLQYLMPPIAGLVAWLTLGE
                     TFTWLKIAGAVAAMAGIAWAQSGAPSRTPPDAG"
     gene            complement(1405..2382)
                     /gene="ltaE"
                     /locus_tag="AC16O9_13400"
     CDS             complement(1405..2382)
                     /gene="ltaE"
                     /locus_tag="AC16O9_13400"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HIR6517645.1"
                     /translation="MPIVDLRSDTVTRPSKGMREAMARAEVGDDVFGDDPTVNRLQDR
                     AAEIFGFEASLFFPTGTQSNLAALMSHCQRGDEVLVGMEAHTYRYEAGGGAVLGSIQP
                     QAIVNRPDGTLDLQDIEAAIKPDDPHFAKTRLLALENTITGRVIARAYFDEALALAKR
                     RGLGTHLDGARIFNAAVKLNTAVKDLCRGFDTVSSCLSKGLGAPAGTMLLGSKDLIAR
                     AKRARKMLGGVMRQAGVLAAAGLYALEHNVERLAEDHANAERLATGIGAPAPSSNMVF
                     FDSKPGLVEHLARHGVIVLPGARLRLVTHLDVDQAGIERAIAAFRSFKP"
     gene            complement(2398..>3234)
                     /locus_tag="AC16O9_13405"
     CDS             complement(2398..>3234)
                     /locus_tag="AC16O9_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6517646.1"
                     /translation="TIMVIYGIITETNIGRLFAAGVLPGVLAVALLCLAVRWSVWRDP
                     KAGPAGERSSWGERLAALKGIWGVIVLFAISIGGIYAGFFTATEGAGVGAAGAFVFAL
                     ARRALTWHTLRDILVESASTTGMLFMILIGALVFANYINFTTLPMDLMGFVEQFSLHP
                     MLVVMSICAIYVILGTAMEELSMILLTVPIFFPLIVALGIDPVYFGILVVVVVEIGLI
                     SPPVGMNLFVLSSLLPQVSTPTLFRGVLPFVTADVVRLFILAAFPAISLWLPSVLNIA
                     GK"
BASE COUNT          483 a         1121 c         1112 g          518 t
ORIGIN      
        1 cgggcgagaa ctcgagcgcg acggtcgcga cccgcgcgtt gaggatcttg gggatctcct
       61 gcaggttcgg ccacagcggc accacctcga ccacgcggtc gcccggatcg accagcagct
      121 gcgacaccag catcagcgcg ttcaccccgg cgctcgtcac caccacctcc cccggcgcgg
      181 tggtccggtg caggcgcgtc aggtaggcgg cgagcgcctc gcgcagctcg gcgatcccgt
      241 agttgctgga atagaacagc tcccccgcct cgatcgaggc gatgccggcc ttgcggatga
      301 actcgggcgt gggctcgtcg ggctcgccca cccagaacgg cacgacgtcc ttgcggccga
      361 tgccggcgtt gaacagctcg cggatcttgg aggcggtcag ctcgcgcacc gcggcgcgcg
      421 ccgcgacacg atcggggctc tcgggtctca tgcggatatt gtagattgcc aggctcggat
      481 ggccgtcgat accgcttccc tgcagaaatg ggcgctcgcc gcgggctgcg cgctgatggt
      541 cacctggggc gtgaacttcg ccatcaccaa ggtggtgctg gccgagctcg gcgtgtggcc
      601 gttcctgttc atccgctttc tcgccatgcc gctggccggt tttggcctgc tcgtcaccat
      661 cacgcggcgc cacttcccca ggacctggcc gcggcgcgag gaccttgcgc gcttcgccgc
      721 cgcggggctc atcggccaca ccgcgcacgt cagcctcgtg atgtggggca tcaacctgtc
      781 cacggcgttc tcgtcttcgc tggtgctgac ctcgagcccg ctatggacgc tcctgatcct
      841 cgcgctgctc ggcgccgaga agctgcacgg gcggcagctc gccgggacgc tcctcgcctt
      901 tgccggcatc gtggtgttcc tgtcggacaa gttcgtgcgg ggcttcgcgc tcgccggcgc
      961 cggcgacctg gtgctgctcg ctgccgcgtc gctgttttcc ctctacacgg tgctcaccaa
     1021 gccgctcgtc gagcgctacg ggccgctcaa cgtgatgtgc tattcgctgc tgttcggcgc
     1081 gccgccgctc gtgctcgcga cgctgcccgc gttcctcgac gcgccgctcg ccggcatctc
     1141 cgccggggtc tggttcggcg tgttctgggc ggtggcggta tcgtccttcc tcggctggat
     1201 cgtgtgggcg tgggtcaact cggtgcgcgg tctcgcgcgc agcgcgccgc tgcagtacct
     1261 gatgccgccg atcgccgggc tggtggcgtg gctcacgctc ggcgagacct ttacctggct
     1321 gaagatcgcc ggcgcggtgg ccgccatggc cggcatcgcc tgggcgcagt ccggcgcgcc
     1381 gagcaggacg ccgcccgacg cgggctaggg cttgaagctg cggaacgccg cgatggcacg
     1441 ctcgatgccg gcctgatcga cgtcgaggtg cgtgacgagg cgcaggcgcg cgcccggcag
     1501 cacgatgacg ccgtggcgcg ccaggtgctc gaccaggccg ggcttcgaat cgaagaacac
     1561 catgttcgag gacggtgccg gcgcgccgat gcccgtcgcg aggcgctcgg cgttggcgtg
     1621 gtcctcggcc aggcgctcga cgttgtgctc gagcgcgtac agccccgccg cggcgagcac
     1681 gcccgcctgg cgcatgacgc cgccgagcat cttgcgcgcg cgcttcgcgc gagcgatcag
     1741 gtccttcgac ccgaggagca tggtccccgc gggcgcgccc aggcccttcg acaggcacga
     1801 ggacaccgtg tcgaagccgc ggcacaggtc tttcaccgcc gtattcagct tcaccgcggc
     1861 gttgaagatg cgcgcgccgt cgagatgcgt gccgagcccg cggcgcttgg ccagcgccag
     1921 cgcttcgtca aagtacgcgc gcgcgatgac ccttccggtg atcgtattct ccagcgcgag
     1981 cagccgcgtc ttcgcgaaat gcgggtcgtc gggcttgatc gccgcctcga tgtcctgcag
     2041 atccagtgtg ccgtcgggcc gattgacgat cgcctggggc tggatcgagc cgagcaccgc
     2101 gccgccgccc gcttcatagc ggtaggtgtg cgcctccatg cccacgagca cctcgtcgcc
     2161 gcgctggcag tggctcatca gcgcggcgag gttggactgg gtgccggtgg gaaagaacag
     2221 cgacgcctcg aagccgaaga tctccgccgc ccggtcctgc agcctgttga cggtgggatc
     2281 gtcgccgaag acatcgtcgc cgacctcggc gcgcgccatc gcctcgcgca tgcccttgga
     2341 gggccgcgtg acggtgtcgc tgcgcaggtc gacaatcggc ataaatgagg tctgtcccta
     2401 ttttcctgcg atgttgagca ccgagggcag ccagagcgag atcgccggga acgcggcgag
     2461 gatgaacagg cgcaccacgt cggcggtgac gaacggcagc acgccgcgga acagcgtcgg
     2521 cgtgctcact tgcggcagca gcgacgagag gacgaacagg ttcatgccta ccggcgggct
     2581 gatgagcccg atctccacca ccaccaccac gagaatacca aagtacaccg ggtcgatgcc
     2641 cagcgcgacg atgagcggga agaagatcgg caccgtgagg aggatcatgg acagctcctc
     2701 catggcggtg ccgaggatca cgtagatcgc gcagatcgac atcaccacca gcatcgggtg
     2761 caggctgaac tgctcgacga agcccatcag gtccatcggc agcgtggtga agttgatgta
     2821 gttggcgaag accagcgccc cgatcaggat catgaacagc atgcccgtgg tgcttgcgga
     2881 ctccaccaga atgtcgcgca gcgtgtgcca ggtcagcgcg cggcgcgcga gcgcgaatac
     2941 gaacgctccg gcggcgccga cgcccgcgcc ttcggtggcg gtaaagaagc ccgcgtagat
     3001 gccgccgatg gagatcgcga acagcacgat cacgccccag attcccttca gcgccgccag
     3061 gcgctcgccc cacgacgagc gctcgccggc gggccccgcc ttcgggtcgc gccaaaccga
     3121 ccagcgcacg gcgaggcaca gcagcgccac cgcgaggacg cccggcagga cgccggcggc
     3181 gaacagacgc ccgatgttgg tctcggtgat gatgccgtag atcaccatga tggt
//
LOCUS       DBNHKE010000196         2496 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_776260, whole genome shotgun sequence.
ACCESSION   DBNHKE010000196 DBNHKE010000000
VERSION     DBNHKE010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_776260"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1120
                     /locus_tag="AC16O9_13410"
     CDS             143..1120
                     /locus_tag="AC16O9_13410"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HIR6517647.1"
                     /translation="MRVKRIAVLTSGGDAPGMNAAIRAVARAATARDWEVFGVRNGYA
                     GLLADAMDPLAARDVGGIIQRGGTILGSARSPEFRERAARDRVRAQLAARGVEALVVI
                     GGNGSQSGSAALAKEGFAVVGVASTIDNDLHGTDVSIGADTAINVTLEAIDRLRTTAS
                     SHRRVFIVETMGRDCGYIALMSGIGGGAEVIAIPESEVPPAEVAARLRAAHERGKTHA
                     MVVVAEGAKQGVHAMVQYFEGRQQETGFGLRITRLGHVVRGGVPSSADRVLASRLGAA
                     AVACLEDGVSGVLVGEVRGAIVRTPLAEIAGRTRPANTALIELARVLAT"
     gene            complement(1113..>2496)
                     /locus_tag="AC16O9_13415"
     CDS             complement(1113..>2496)
                     /locus_tag="AC16O9_13415"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030980 - alpha-glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HIR6517648.1"
                     /translation="PVYRTYIIDAASDQDLRYVNWAVAHARRRSRAADVSIFDFVRQS
                     LLGQTVEGAEEGLRALVRRFAIRFQQFTSPVAAKGVEDTAFYLYGRLASLNEVGGDPA
                     QFGMTLRAFHGASADRAARWPHTMLATSTHDNKRAEDVRCRIDVLSEIPAGWRLALRR
                     WGALNRAHRAKLDSGPAPSAVDEYLLYQTLLGTLPPGTLDEAALAAWRERIERYMIKA
                     AREAKANTSWISPNEEYENALTAFIRALLGRLAPNPFLEELRAQAEPIAWFGALNSLS
                     MTLVKFASPGVPDIYQGTELMDLSLVDPDNRRPVDYARRAALLEELGALADPRPLLDA
                     PHDGRAKLWIAWRMLELRRKLPELFRDGGYAPLAASGKHAAHVLAFARTHASGTLVVV
                     AGRLHAKMLRDAGKLPLGEAWADTAIALPPGVTTLQNVLTGERLDALGGLLPLARACT
                     RFPVAALLAT"
BASE COUNT          336 a          863 c          968 g          329 t
ORIGIN      
        1 acatccgcca ggcggccgag gacgtcgatc gcatccagac caccggcggc aagatctcgc
       61 agcagttcca gagaatcgag gccgccgagg tggacgacgc gcccgtcgcc gaggtggtgc
      121 ggctggagga ggggcgcaag ccgtgagggt gaagcgcatc gcggtcctca ccagcggcgg
      181 cgacgctccc ggcatgaacg ccgccatccg cgccgtggcg cgcgcggcga ctgccaggga
      241 ctgggaagtg ttcggcgtgc gtaacggcta cgccggcctg ctcgccgacg ccatggaccc
      301 gctggccgcg cgcgacgtgg gcggcatcat tcagcgcggc ggcacgatcc tcggcagcgc
      361 gcgctcgccc gaatttcgcg agcgcgccgc gcgcgaccgc gtgcgcgcgc agctcgccgc
      421 gcgcggcgtc gaggcgctgg tggtgatcgg cggcaacggc tcgcagagcg gctcggcggc
      481 gctcgcgaaa gagggctttg cggtggtggg cgtcgcctcg accatcgaca acgatctgca
      541 cgggaccgac gtgtccatcg gcgcggacac ggcgatcaac gtcacgctgg aggcgatcga
      601 ccggctgcgc accaccgcgt cctcgcaccg gcgcgtgttc atcgtcgaga ccatgggccg
      661 cgactgcggc tacatcgcgc tgatgtcggg catcggcggc ggcgccgagg tgatcgcgat
      721 tcccgagagc gaggtgccgc ccgccgaggt cgcggcgcgc ctgcgcgccg cgcacgagcg
      781 cggcaagacc cacgccatgg tggtggtggc cgagggcgcg aagcagggcg tgcacgccat
      841 ggtgcagtac ttcgagggcc ggcagcagga gacgggcttc ggcctgcgca tcacgcgcct
      901 ggggcacgtg gtgcgcggcg gcgtgccgag ctccgcggat cgcgtgctcg cctcgcgcct
      961 gggcgcggcg gcggtggcgt gtctcgagga cggcgtgtcc ggcgtgctgg tgggcgaggt
     1021 gcgcggcgcg atcgtgcgca cgccgctcgc cgagatcgcc gggcgcacgc ggcccgccaa
     1081 caccgcgctg atcgagctgg cgcgcgtact cgctacgtag cgagcagcgc cgccaccggg
     1141 aagcgagtgc aagcgcgtgc cagcggcagc aggccgccga gcgcgtcgag ccgctcgccg
     1201 gtaagcacgt tctggagcgt cgtcacgccc gggggcagcg cgatcgccgt atccgcccac
     1261 gcctcaccca gcggcagctt gccggcgtcg cgaagcatct tcgcgtgcag ccgtcccgcc
     1321 accaccacca gcgtgccgct tgcgtgcgtg cgcgcgaagg cgaggacgtg cgcggcgtgc
     1381 ttgccgctcg cggcgagcgg ggcgtagccg ccgtcgcgga acagctcggg cagcttgcgg
     1441 cgcaactcga gcatgcgcca ggcgatccag agcttggcgc ggccgtcgtg cggcgcatcg
     1501 agcagcggcc gcgggtcggc gagcgccccg agttcctcga gcagcgccgc gcgccgcgca
     1561 tagtccaccg gccggcggtt gtcggggtcg accaggctca ggtccatgag ctcggtgccc
     1621 tggtagatgt cgggcacgcc cggcgaggcg aacttgacga gggtcatgct gaggctgttc
     1681 agcgcgccga accaggcgat cggctcggcc tgtgcgcgca gctcctcgag gaacgggttg
     1741 ggcgcgagcc ggccgagcag cgcgcgtatg aacgcggtga gcgcgttctc gtactcctcg
     1801 ttcgggctga tccagctggt gttggctttc gcctcgcgcg ccgccttgat catgtagcgc
     1861 tcgatgcgct cgcgccaggc ggcgagcgcc gcctcatcga gcgtgcccgg cggcagggtg
     1921 ccgaggaggg tctggtagag caggtactcg tccacggcgg agggcgccgg gcccgaatcg
     1981 agcttggcgc ggtgcgcgcg gttcagcgcg ccccagcgcc gcagcgcgag gcgccagccg
     2041 gccgggatct cggagagcac gtcgatgcgg cagcgcacgt cctcggcgcg cttgttgtcg
     2101 tgcgtggagg tggcgagcat ggtgtgcggc cagcgcgcgg cgcggtcggc gctcgcgccg
     2161 tggaaggcgc gcagcgtcat gccgaactgc gccgggtcgc cgccgacctc gttcagcgac
     2221 gcgagcctcc cgtagaggta gaacgcggtg tcctcgacgc cctttgcggc gaccggcgaa
     2281 gtgaactgct ggaagcggat ggcgaagcgc cgcaccaggg cgcgcagccc ctcttcggcg
     2341 ccctccaccg tctggccgag gagcgactgg cgcacgaaat cgaagatcga cacgtcggcc
     2401 gcccggctgc ggcgccgggc atgcgccacc gcccagttga cgtagcgcag gtcctggtcc
     2461 gaggccgcgt cgatgatgta ggtgcggtac accggc
//
LOCUS       DBNHKE010000197         6851 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_935403, whole genome shotgun sequence.
ACCESSION   DBNHKE010000197 DBNHKE010000000
VERSION     DBNHKE010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_935403"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC16O9_13420"
     CDS             <1..366
                     /locus_tag="AC16O9_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517649.1"
                     /translation="GCVQVRGQRKLPATPLVVIAGEIDPSCSSSGRHFMQAPVEAESP
                     ETTITLLGFVLNVSAPTDTPPYKDVNEAGLTRTAFFNAVSPAGTNAAGLPVPGTLVKV
                     IFDTGGPSPNAVRQVEIED"
     gene            complement(620..934)
                     /locus_tag="AC16O9_13425"
     CDS             complement(620..934)
                     /locus_tag="AC16O9_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR6517650.1"
                     /translation="MAESGCSELEPYALRVLGESMAPEFPEGCIIIVDPGHVARHGSY
                     VVVEYAGDVFFRQLVIEDERRFLEALNPKYGRFELIPPYTIRGGVVQRAGRRRAERKH
                     YG"
     gene            complement(927..1214)
                     /locus_tag="AC16O9_13430"
     CDS             complement(927..1214)
                     /locus_tag="AC16O9_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517651.1"
                     /translation="MSEPELSKEERILRAMKLTLAGVIRDTATPHGMRHPLSEATIED
                     LRQCLALISARERELAAEAGRDASARPRYADEPSRAEQVISFYRKKEDGDG"
     gene            complement(1275..3023)
                     /locus_tag="AC16O9_13435"
     CDS             complement(1275..3023)
                     /locus_tag="AC16O9_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6517652.1"
                     /translation="MSQSSVPMHGGERVAAALAAHGVRFLFTLCGGHISPILVAAKRR
                     GIRIVDTRDEATAVFAADAVARLTGTPGVAAVTAGPGLTNTITALKNAQLAQSPVVLL
                     GGAAPTALQGRGALQDIDQHRLIAPHVKRVTRVRNVAALGPAVEQALVIARDGVPGPA
                     FVECPVDLLYDETLIRGWYRDAAGRGTSIADRLLRWYLNRHANRMFAGSARPYRPAAA
                     TVATPEAGTGAVARAAEALRRARQPLMVLGSQAVVAASEAPRVAEAVARLGVPVYLSG
                     MARGVLGRDTGLQMRHARRAALREADVVLLAGVPCDFRLDYGKHVRRSATLIAANRSR
                     KDARLNRRPSIEAVGDAGRFIVALAQGLGDGARRGEWIGHLHERDAEREREIEAQART
                     SGEHVNPVAFFRALDRTAAEHAVFVADGGDFVATASYLLRPRGPLAWLDPGAFGTLGV
                     GAGFALGASVVRPAAEVWLLWGDGACGYGLAELDTFVRHGIPVIAVVGNDAGWTQIAR
                     EQVKLLGDDVGTTLARTAYHEVAKGFGAEGLEIRRDADVEAGLAQARRIAATGKPVLV
                     NVRLDKTGFREGSISM"
     gene            complement(3025..3375)
                     /locus_tag="AC16O9_13440"
     CDS             complement(3025..3375)
                     /locus_tag="AC16O9_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6517653.1"
                     /translation="MPRSRSTKRARRGAPKTDDPAAILFCGGTTGLPKGIVLSNRNFI
                     AQGMQAAAWCEPGERDAIRAILPIFHGFGLGVCVNAAFMAGGKSILVPRFSAQVGKVD
                     YRELVRRHAAKERA"
     gene            complement(3320..3523)
                     /locus_tag="AC16O9_13445"
     CDS             complement(3320..3523)
                     /locus_tag="AC16O9_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517654.1"
                     /translation="MIDWRSTRRRARRSKLVPIEPRAALGLGPLMHEDRPWLRFYGKV
                     PATLAYPEVTLYEARAARRAEDR"
     gene            3714..4460
                     /locus_tag="AC16O9_13450"
     CDS             3714..4460
                     /locus_tag="AC16O9_13450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6517655.1"
                     /translation="MDVIARERYDAVLFDLDGVLTDTAQIHASSWKRMFDAYLQGRAR
                     RTGEAFRPFDIATDYRQLVDGKPRYDGVRDFLRARGIDLPEGTPQDAPGTETVCGLGN
                     RKNDLVNAAIAAGEVATFPASVALVRRLRQNGIKCAVVTSSRNLGAVLEAAGIADLFE
                     AAVDGNTIAALGLAGKPAPDAYLQAAKALGVAPARAVVVEDAISGVAAGANGGFGLVI
                     GVARHGNAEELKRHGAHLVVAGLGELTDRS"
     gene            4467..6845
                     /locus_tag="AC16O9_13455"
     CDS             4467..6845
                     /locus_tag="AC16O9_13455"
                     /inference="COORDINATES: protein motif:HMM:NF015588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIR6517656.1"
                     /translation="MRHHERLKPPPHDYVADEWNWIERRFHPEFLAQTETILALGNGY
                     LGMRGSPEEGGPLAQFGTYVNGFHETWPIVYGEDAFGFARTGQTMVSVTDAKIIKLFV
                     DDEPFWLATANLLRFDRRLNMRAGTLDREVLWETPAGKQVHIKSRRLISFEHRHAGAI
                     SYEVTLLNARAPVVISSEVLAERRSTPRGSGDPRRSKAFSGRVLQPRASHAGERRLVL
                     CHGTERSGMLLACGVDHHLESECQHAWKTAHTEDFGQVAFTIAAEPGRPIRLTKFMAH
                     HSTATASPEQLCARVDWTLNRIAAEGFDALAAGQQRYMDEFWRRSDVQVQNVRTGKVQ
                     RTTAEVQQAIRFNLFHILQACARAENVGVPAKGLTGNAYEGQYFWDTEIYVLPFLIYT
                     APRLARNQLLFRYRMLGKARERAQELNHKGALFPWRTITGEEASANYVAGTAQYHINA
                     DIMYALRKYVHATGDEEFLRQYGAEMLIETARFWYDIGFFSERKGGRFCINGVTGPDE
                     YTTVVNNNTYTNLMARENLRYAAQTIEALRDGNPHTLAALAHKTAFDLAEVEDWKRAA
                     ERMFIPYDEQSGIYLQDESFLDRERWDFENTPADKYPLLLYYHPLNIYRHQVIKQADV
                     ILAMFLLGHEFTLEEKRNNFLFYAPLTTGDSSLSSCVEAVIAAEIGEHERATEYAMAA
                     LLMDLGDVSGNVHDGCHVASMGGTWMVMVYGFGGMRDSDGMLSFRPSAAPQGQSTIRF
                     PLTYRGRLLEVEVGVHETRYALREGDQIAFRHNDEVIELTRANPVATRPNVK"
BASE COUNT         1057 a         2436 c         2371 g          987 t
ORIGIN      
        1 ggctgtgttc aggtccgtgg acagcgcaag ctgcctgcaa cgccgctggt ggtcattgca
       61 ggcgaaatcg atccttcctg cagcagcagc ggtcgacatt tcatgcaagc gccggtggag
      121 gcggagagcc ctgagaccac gattacgttg ctcgggtttg tgttgaacgt gagcgcgccg
      181 acggacactc cgccctacaa ggacgtcaat gaggctggcc ttacccgcac ggcgttcttc
      241 aatgcggtgt cacccgctgg aaccaatgcg gccggcctgc cggttccggg caccctggtg
      301 aaagtgatct ttgataccgg cggcccgagt cctaacgccg ttcgacaggt cgagatcgaa
      361 gactgacggc tgaccggctg caccaagacg ccgccccaac agggcggcgt tcttttttcc
      421 cgggtcgttc cgcccggagg agagcagagc cacgaccgag aaacggcgag cgcgtgcggg
      481 tggcacggat cgcatccagg cacttctcca gcccgccatc catggcgtgc gcggcgagcg
      541 caacgatcgg caccgctccg gcatcctgga tgcgccgcgt cgccccggcg ccgtccatcg
      601 ccgcccccgg ggcggcttct cagccgtagt gcttgcgctc ggcgcggcgc cgccccgcgc
      661 gctgcaccac cccgccgcgg atcgtgtagg gcgggatcag ctcgaagcgg ccgtacttcg
      721 ggttgagcgc ctcgaggaac cgccgctcgt cctcgatcac cagctgccgg aagaagacgt
      781 cgcccgcgta ctccacgacc acgtacgagc cgtgcctcgc gacgtggccc gggtcgacga
      841 tgatgatgca gccctcgggg aactccggcg ccatgctctc gcccagcacg cgcagcgcgt
      901 agggctcgag ctcgctgcag ccgctctcag ccatcgccgt cctctttctt gcggtagaag
      961 ctgatgacct gctcggcgcg cgagggctcg tcggcgtagc gcgggcgcgc ggaggcatcg
     1021 cggcccgcct ccgcggcgag ctcgcgctcg cgcgcgctga tcagcgccag gcactggcgc
     1081 aggtcctcga tggtcgcttc cgacagggga tggcgcatgc cgtgcggcgt cgcggtgtcg
     1141 cgaatgacgc cggcgagcgt gagcttcatc gcccgcagga tgcgctcctc cttcgacagc
     1201 tccggttcgc tcataggtcc gatcatatgc ggatcaggtt cgacgcggcg gcgcgcacgg
     1261 cgcgaaccgc gagcctacat cgagatcgag ccctcccgga acccggtctt gtcgagccgg
     1321 acgttgacga gcaccggctt gccggtggcc gcgatgcgcc gcgcctgcgc gagccccgcc
     1381 tcgacgtccg catcgcgccg gatctccagg ccctcggcgc cgaagccctt cgccacctcg
     1441 tggtaggcgg tgcgcgcgag ggtcgtgccc acgtcgtcgc cgagcagctt cacctgctcg
     1501 cgcgcgatct gcgtccatcc cgcgtcgttg cccaccaccg cgatcaccgg gatgccgtgg
     1561 cgcacgaagg tgtcgagctc cgccagcccg taaccgcacg cgccgtcgcc ccagaggagc
     1621 cacacctcgg cggcgggcct gacgaccgag gcgccgagcg cgaagcccgc gccgaccccg
     1681 agcgtgccga acgcgcccgg atccagccag gcgagcggcc cgcgcgggcg caggaggtag
     1741 gaggcggtgg cgacgaaatc gccgccgtcc gccacgaaga ccgcgtgctc tgccgcggtc
     1801 ctgtcgagcg cgcgaaagaa cgccaccggg ttcacgtgct cccccgaagt ccgcgcctgg
     1861 gcttcgattt cgcgctcgcg ctcggcatcg cgctcgtgca ggtgcccgat ccactccccg
     1921 cgccttgccc cgtcgccgag accctgcgcc agcgccacga tgaagcggcc cgcgtcgccg
     1981 accgcctcga tcgatggcct gcgattcagg cgcgcatcct tgcggctgcg gttggcggcg
     2041 atgagcgtgg ccgagcgccg cacgtgcttg ccgtagtcca ggcggaagtc gcacggcacg
     2101 ccggcaagca gcacgacgtc cgcttcgcgc aatgccgccc ttcgcgcatg gcgcatctgc
     2161 aggccggtgt cacgcccgag cacgccccgc gccatgcccg acaggtagac cggcaccccg
     2221 agccgtgcca cggcctcggc cacgcgcggc gcttcgcttg cggcgacgac ggcctggctg
     2281 ccaagcacca tcagcggctg acgtgcccgg cgcagcgcct cggcggcacg cgccaccgcc
     2341 cccgttcccg cctcgggcgt cgccaccgtg gccgccgccg gccgataggg ccgcgcgctg
     2401 cccgcgaaca tgcggttggc gtggcggttc aggtaccagc gcagcagccg gtcggcaatc
     2461 gaggtgccgc gcccggccgc gtcgcggtac cagccgcgga tgagcgtctc gtcgtacaga
     2521 aggtccacgg ggcattccac gaaggcgggg ccgggcacgc cgtcgcgcgc gatgacgagc
     2581 gcctgctcga ccgcggggcc gagcgcggcc acgttgcgca cgcgcgtgac gcgcttcacg
     2641 tggggcgcga tcagccgatg ctgatcgatg tcctgcagcg cgcccctgcc ctgcagggcc
     2701 gtgggcgccg cgccgccgag cagcaccacg ggcgactgcg cgagctgcgc gttcttcagc
     2761 gcggtgatcg tgttggtgag gccggggccc gcggtcaccg ccgccacgcc cggcgttccg
     2821 gtcaggcgcg ccacggcatc ggccgcgaag accgcggtcg cctcgtcgcg cgtatcgacg
     2881 atgcggatgc cgcgccgctt ggccgcgacc aggatgggcg agatgtggcc gccgcagagc
     2941 gtgaacagga agcgcacgcc atgggcggcg agtgccgccg cgacgcgctc gccgccatgc
     3001 atggggacgc tcgactgact catgtcaggc ccgctccttc gccgcgtggc ggcgcaccag
     3061 ctcgcgataa tcgaccttgc ccacttgcgc gctgaaccgc ggcacgagga tcgacttgcc
     3121 gcccgccatg aaggccgcgt tcacgcacac cccgagcccg aagccgtgga agatcggcag
     3181 gatggcgcgg atggcgtcgc gctcgcccgg ctcgcaccag gccgcggcct gcatgccttg
     3241 cgcgatgaaa ttgcggttcg agagcacgat gcccttgggc agaccggtcg tgcctccgca
     3301 gaagaggatc gctgcggggt catcggtctt cggcgcgccg cgccgcgcgc gcttcgtaga
     3361 gcgtgacctc gggatacgcg agggtggccg gaaccttgcc gtagaagcga agccaggggc
     3421 gatcttcgtg catgaggggt ccgaggccga gcgccgcgcg cggctcaatc ggcacaagct
     3481 tactcctgcg cgcccggcgc cgcgtgctgc gccagtcgat cacgatgcca cggtggtcgt
     3541 tcgcgcatcc ttgaggcttc tcgccccgcg ggcggcgggc gtgagcgagc gctacggccg
     3601 gccgcgccgg tcgcactgct tatacttggc gtcgtgccct tcgcacccgg agagccgacc
     3661 gcccgccgcg catgcgctcg cgtgcgccgc atcgaccaag ccgtgacgcg gccgtggacg
     3721 tgatcgcgcg cgagcgttac gacgcggtgc tgttcgatct ggacggcgtg ctcaccgaca
     3781 ccgcgcagat ccatgcgtcg agctggaagc gcatgttcga tgcgtatctg cagggccgcg
     3841 cgcggcgcac cggcgaggca ttccgcccgt tcgacatcgc caccgactac cggcagctcg
     3901 tcgacggcaa gccgcgctac gacggggtgc gcgatttcct gcgcgcacgg ggcatcgatc
     3961 tcccggaagg cacgccgcag gacgcgccgg gcacggagac ggtctgcgga ctgggcaacc
     4021 gcaagaacga cctggtcaac gccgccatcg ccgcgggcga ggtcgcgacc ttcccggcgt
     4081 cggtggcgct ggtgcgcagg ctgcgccaga atggcatcaa gtgcgcggtc gtcacctcga
     4141 gccgcaacct tggcgcggtt ctcgaggccg cgggcatcgc cgatctgttc gaagcggcgg
     4201 tggacggcaa tacgatcgcc gcgctcggac tcgcgggcaa gccggccccc gacgcgtacc
     4261 tgcaggcggc aaaggcgctc ggcgtggccc cggcgcgcgc ggtcgtggtc gaagacgcga
     4321 tctcgggcgt cgcggccggc gcgaacggcg gcttcggcct ggtgatcggc gtcgcgcgcc
     4381 acggcaacgc cgaggaattg aagcggcacg gcgcccacct cgtggtcgcc ggccttggcg
     4441 agctgaccga ccggagctaa agcgcgatgc gccaccacga acgcctcaaa cctccgccgc
     4501 acgactacgt ggccgacgag tggaactgga tcgagcgccg gttccacccc gagttcctgg
     4561 cgcagaccga gaccatcctc gcgctcggca acggttacct cggcatgcgc ggctcgccgg
     4621 aagagggcgg cccgctcgcg cagttcggca cctacgtcaa cggattccac gaaacctggc
     4681 cgatcgtcta cggcgaggac gctttcgggt tcgcgcgcac cggccagacg atggtgagcg
     4741 tgacggacgc gaagatcatc aagctgttcg tggacgacga gcctttctgg ctcgcgaccg
     4801 cgaacctgct gcgcttcgac cggcgcctga acatgcgcgc gggcacgctc gatcgcgagg
     4861 ttctgtggga aacgccggcc ggcaagcagg tgcacatcaa gtcgcggcgc ctgatctcct
     4921 tcgagcaccg ccacgccggg gcgatctcgt acgaggtgac gttgctcaac gcgcgcgcgc
     4981 cggtcgtgat ctcctccgag gtgctggccg agcgccgcag cacgccgcgc ggcagcggcg
     5041 atccgcggcg cagcaaggcg ttctcggggc gggtcctgca gcctcgcgcg agccacgccg
     5101 gcgagcggcg cctggtcctg tgccacggca ccgagcgctc ggggatgctg ctggcctgcg
     5161 gcgtcgacca ccacctcgaa tcggagtgcc agcacgcgtg gaagaccgcg cacacggagg
     5221 atttcggcca ggtggcgttc acgattgccg ccgagcccgg gcgcccgatc cgcctcacga
     5281 agttcatggc gcaccacagc accgccacgg cgtcgcccga gcagctgtgc gcgcgcgtcg
     5341 actggacgct gaaccgcatc gccgcggagg ggttcgacgc gctcgccgcg ggccagcagc
     5401 gctacatgga cgagttctgg cggcgcagcg acgtgcaggt gcagaacgtg cgcacgggaa
     5461 aggtgcagcg cacgaccgcc gaggtgcagc aggcgatccg cttcaatctg ttccatatcc
     5521 tgcaggcctg cgcgcgcgcg gagaacgtgg gcgttcccgc caaggggctg acggggaacg
     5581 cctacgaggg gcagtacttc tgggacaccg agatctacgt gctgccgttc ctcatctaca
     5641 ccgcgccgcg cctggcgcgc aaccagctgc tgttccgcta ccgcatgctc ggcaaggcgc
     5701 gcgaacgcgc gcaggagctc aatcacaagg gcgcgctctt tccgtggcgc acgatcacgg
     5761 gcgaggaggc ttccgcgaac tacgtggcgg gcaccgccca gtaccacatc aatgccgaca
     5821 tcatgtacgc gctgcgcaag tacgtgcacg cgacgggcga cgaggagttc ctgcggcagt
     5881 acggcgccga gatgctcatc gagaccgcgc gcttctggta cgacatcggc tttttttcgg
     5941 agcgcaaggg cgggcgcttc tgcatcaacg gcgtgaccgg gcccgacgag tacaccacgg
     6001 tggtcaacaa caacacctac accaatctca tggcacgcga gaacctgcgc tacgccgcgc
     6061 agaccatcga ggcgctgcgc gacgggaatc cgcataccct tgcggcgctc gcgcacaaga
     6121 cggcgttcga tctcgcggag gtcgaggact ggaaacgggc tgccgagcgc atgttcatcc
     6181 cctacgacga gcagagcgga atctatctgc aggacgagag ctttctcgac cgggagcggt
     6241 gggatttcga gaacacgccc gccgacaagt acccgctgct gctgtactac cacccgctga
     6301 acatctaccg gcaccaggtg atcaagcagg ccgacgtgat cctggcgatg ttcctgctcg
     6361 ggcacgagtt cacgctcgag gagaagcgca acaactttct cttctatgcg ccgctgacga
     6421 ccggcgattc gtcgctgtcg tcctgcgtcg aggccgtgat cgcggccgag atcggcgagc
     6481 acgaacgggc gaccgagtac gccatggcgg cgctgctcat ggacctgggc gacgtgagcg
     6541 gcaacgtgca cgacggctgc cacgtcgcct cgatgggcgg cacctggatg gtgatggtct
     6601 acgggttcgg gggcatgcgc gacagcgacg gcatgctgtc tttccggccg agcgccgcgc
     6661 cgcaagggca atcgacgatc cgctttccct tgacttaccg cgggcgcctg ctggaagtcg
     6721 aggtcggcgt gcacgagacg cgctacgcat tgcgcgaagg cgaccaaatc gcgttccggc
     6781 acaacgacga ggtgatcgag ctgacgcgcg ccaatccggt cgcgacgcgg cccaacgtca
     6841 agtagggccg c
//
LOCUS       DBNHKE010000198        31249 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_35802, whole genome shotgun sequence.
ACCESSION   DBNHKE010000198 DBNHKE010000000
VERSION     DBNHKE010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 31249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31249
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_35802"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1438
                     /gene="pcnB"
                     /locus_tag="AC16O9_13460"
     CDS             116..1438
                     /gene="pcnB"
                     /locus_tag="AC16O9_13460"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR6517657.1"
                     /translation="MIKRFIRRVLGLPSHAPQRVPRARHPLRRAALSHAALKVCDVLR
                     DAGHSASVVGGAVRDLLLDIVPKDYDVATDARPEQVKPLFRRALIIGRRFRLVHVMLG
                     PETVEVSTFRAADAGTTEKDEHGRVLRDNVFGSQEEDARRRDFTVNALYFDPATEEII
                     DYHGGLADLRRHVLRMIGDPETRYREDPVRMLRAVRLAAKLGLTIEAGTRAPIRRLAP
                     LIEHVPPARVFDEMLKLLLSGHASACVRQLREEGLSKGVLPLLDVILEQPLGERFVTL
                     ALAQTDERVRTDRSVSPAFLFAALLWHEVLAAWKARLAKGERPLPALEAAMDRVLDAQ
                     CEKLALTRKLTATMREIWAMQPRFEQRGGQRPSRLLEAPRFRMGYDFLALRAASGEVS
                     AELEAWWRAFQQADADSRRAMLLPDTGPRKKRRRRRRRQDAAATTEQA"
     gene            1438..1914
                     /gene="folK"
                     /locus_tag="AC16O9_13465"
     CDS             1438..1914
                     /gene="folK"
                     /locus_tag="AC16O9_13465"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6517658.1"
                     /translation="MTAYIGLGANQGEPRAQIDQAFAELAALPATRLAARSPLYRSAP
                     LDAPGQPDYVNAVAAVDTDLSAPQLLDALQAIEARHGRERPHRNAPRTLDLDLLLFGE
                     TAFASPTLTLPHPRMHERAFVLRPLLDLDPRAEVPGRGSARALLRACAAQRIERIG"
     gene            1915..2547
                     /locus_tag="AC16O9_13470"
     CDS             1915..2547
                     /locus_tag="AC16O9_13470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6517659.1"
                     /translation="MERYRYIAVEGPIGSGKTSLARRLAARLGADLLLEQPAENPFLA
                     RFYEDMARYALPTQLFFLFQRARQLEPLAQPDMFGRATVADFLIDKDPLFARITLSGD
                     ELTLYQRIYEALRPQAPAPDLVIYLQAQPQVLIERVRRRAAAYERGIGEEYLALLAEG
                     YARFFYHYAAAPVLIVNSENLNFVERDADFDLLVSRMRAMKSRREYFNLG"
     gene            complement(2557..2793)
                     /locus_tag="AC16O9_13475"
     CDS             complement(2557..2793)
                     /locus_tag="AC16O9_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517660.1"
                     /translation="MYAIVHKSDGFPICRQVAGVSPDPVVTWNTEDAAKAFISAKGGD
                     KDFSPVALTDDAMDRIAASMGCPVESITFDPYPA"
     gene            3281..4213
                     /locus_tag="AC16O9_13480"
     CDS             3281..4213
                     /locus_tag="AC16O9_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6517661.1"
                     /translation="MSNMFDAVGLRAPCTRRYTELEYEFEPVSGTLWGFMKQRGNACF
                     NLGILKDIRDHDEALSANGGCVDFEGTLQRVNWYVAASRVPKVFNLGGDLALFLLLIK
                     ARDRDALAHYARLCIDNVYARITNYGCPNVTTISLVQGDALGGGFETALSSDVIIAEE
                     SAQMGLPEIIFNLFPGMGAYSLLARRIGMRAAEDLILSGRVLSAAKLHEMGVVDVLAK
                     DGEGETAVHAWIAANAKRRNGLQGVLRSRQHVFPITRAELDAIGDTWVDCALRLEDRD
                     LRMMGRVVRAQMRRLEEGGITDIIAEAEALAAAG"
     gene            complement(4218..4883)
                     /locus_tag="AC16O9_13485"
     CDS             complement(4218..4883)
                     /locus_tag="AC16O9_13485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR6517662.1"
                     /translation="MSFFIQLVESTDASRRALEAEPRVHAMVHHGLTLPEYLAFLHDL
                     YHIVWHFCPVMAAAVARCGDDFRDVRFELYGRIEEEKGHEGWVLEDIEAMGGDVSATR
                     AGLPSAPVQAMIAFNYYAAEHVHPCSVLGMLYVLEVIASVYGGRVSDAIARKLGRDVA
                     AGGFKFLSSHATMDLDHMASLNRLVKTILDPAAQAAIIRSTRVNFHQFSQVFRTGGPL
                     GPA"
     gene            4940..5878
                     /locus_tag="AC16O9_13490"
     CDS             4940..5878
                     /locus_tag="AC16O9_13490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6517663.1"
                     /translation="MDPIRLYLLILPVFLAAIAVEALYYRVALHRPYGWKVTVSNLAV
                     AIGRLGTDAFTKALVAAVYVAAYQLRPFDIPLDRWESWVALFLAVELAYYWLHRFSHE
                     IRWMWAQHSVHHSARQITLSVAYRLGWTQFFAGPWLFLVPVCWIGFDPRAVVLMYAIN
                     LLYQFWLHTEVVPKLGPLEAVLNTPSLHRVHHAVEPEYLDRNYGGVLIIWDRLFGTYA
                     KERDGAPRTYGLVRQIDTLNPVKIAFAEWVAMFRELRTARSLREALGLAFGPPGWKAD
                     GSGQTTRMIRRAAGIPENSRARAPVAALAAERDAPR"
     gene            complement(5897..6808)
                     /locus_tag="AC16O9_13495"
     CDS             complement(5897..6808)
                     /locus_tag="AC16O9_13495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6517664.1"
                     /translation="MAGSDRLEPAVDERKLRIRAADGYELGAILYSVPTEAAPPRAAV
                     LHGGGGIPALSYRRFARFLARSGIPVLTYDFRGIGLSRPPALRGFQAVIEDWAEHDCS
                     AAIGWLRERFPRTELIGIAHSVAALLFGGAHNTAEQARMILIGAHTGYYGDYLLRYRL
                     PMAMLWHGLMPALTRLVGYFPARRLGLGDDMPAGVALQWAARHSPDLRPAGASPAAER
                     TRRLLDRCAALDRPALVIRISDDAFATAEGTRRLLAYYPRLSPQHLLFTPADAGVRKI
                     GHFGFFSRRAGLALWPRLVAWLRPPDS"
     gene            complement(6893..9418)
                     /locus_tag="AC16O9_13500"
     CDS             complement(6893..9418)
                     /locus_tag="AC16O9_13500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6517665.1"
                     /translation="MKLPGAVWWRWLRVRLAGRADTEHEQAIVRLVVGVLLGLYLLPE
                     ILQRHAEGLPEPHIVVWLGFLALSTVIFGGILASPGVSVARRIAGMVLDTAATSWCIA
                     YFQELGIPLFLVYLWFTIGNGFRYGARYLVISMMLSMLGFAIALNVSEYWQARTSVGT
                     GMMVGFVALSLYVLTLVRRMFDAVARAEAANEAKRRFVSVVSHEMRTPLNAIIGMADL
                     MRDTALTREQADMLQTLRGSSQIMLGLVEDVLDFSKIEAGKLVLERTDFDLHALVNST
                     SRILQAQAKAKGVEFVVSIMPEVPPALRGDAHHLRQVLINLAGNAVKFTERGSVTVHV
                     SLQEETETSVRLKFSVRDTGIGIAQEAQRTIFESFTQADQSTTRRFGGTGLGTTIAKQ
                     LVELMGGKIGLESAVGLGSTFWFDITYEKQPERPSAGAGELAGARILLVGMPAHQAQP
                     IEEALEAWGAMGVRAASVEEGADRLITDIALAKPYHSVLMYSEGGDTQLARRLRRAAP
                     EPAPPAVLAVPREAEAQRFSALSAGFSAVLELPFEKRQLFNVLHAISSSHELREGVVR
                     LQDYAKRGQGARGLSVLVADDNPTNREVLGRILERAGHAVSLVADGDQALDALETGHY
                     DIVLLDRNMPGLSGLETLQAIRLMTRGRERLPVILLSADVTLEAKRECLEAGADSFVP
                     KPVEALRLLEEMRSLTGGKPQEAARAAPAAVAVAAPTGAEPVNIETLARLEELGSTPD
                     FLERLVGVFVADSQSLLAKMEAAVAARNFGEFRAHLHAMKGSAASIGTERLTWLCSKL
                     GKYSDAELRLQSSSLVRSLNEEFAQGRSALERYIREKRSRSAG"
     gene            complement(9415..10506)
                     /locus_tag="AC16O9_13505"
     CDS             complement(9415..10506)
                     /locus_tag="AC16O9_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6517666.1"
                     /translation="MAKAQVFVMPAARNTAMVVDDQTTGRAILEQVVRSLDERVGVEG
                     FGRPVDAVVWATRNVADLVLIDYMMPDMNGIEFAKRLRALPGYEHVPIMMVTAHDDRK
                     VRYDALDAGVTDFLIKPVDARECMARCRNLLTLRRQHLALEDRRRLLEGMVDEATREV
                     RNREKETLMRLARAGEFRDEETGYHLVRMARYSRLIADTIGLERDEAESIELAAPLHD
                     IGKIGIPDQILLKSGRLDEAESKVMRRHPLIGHEILKGSASKYVRMGSLIALGHHEKY
                     DGSGYPNGLVGDHIALCARVVAVADVYDALTSIRPYKQAWSADQAFEFLDTQRGKHFD
                     PQLVDAFSGVRGAVARIQDELRDPEAKRS"
     gene            complement(10727..10813)
                     /locus_tag="AC16O9_13510"
     tRNA            complement(10727..10813)
                     /locus_tag="AC16O9_13510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10777..10779),aa:Leu,seq:caa)
     gene            10853..11851
                     /gene="queA"
                     /locus_tag="AC16O9_13515"
     CDS             10853..11851
                     /gene="queA"
                     /locus_tag="AC16O9_13515"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR6517667.1"
                     /translation="MRLAEFDYALPEELIAQRPAPERTASRLLHLDPASGRLRDLKFA
                     DLPSLVGTRDTLVLNDTRVIKARLAGKKASGGRIELFIERVLGEREALALIRSSHAPR
                     AGNELVVGDGARVTVVGREDDLYRVRFAGALSAVLERSGEVPLPPYIRHAPDAADAER
                     YQTVYAERPGAVAAPTAGLHFDRAMLQRIEERGARIARVTLHVGAGTFQPVRVENIEA
                     HSMHRERYDVPRLTMDAIAGRRVLAVGTTSLRALESAALTGQLHGDTDLFVRPGFQFL
                     LVDRLLTNFHLPRSSLLMLVAAFAGLENIRRAYAHAIEQRYRFFSYGDAMLIERAP"
     gene            11848..12951
                     /gene="tgt"
                     /locus_tag="AC16O9_13520"
     CDS             11848..12951
                     /gene="tgt"
                     /locus_tag="AC16O9_13520"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR6517668.1"
                     /translation="MKFTLSHRDGAARRGRLELAHGVVDTPAFMPVGTYGTVKAMSPA
                     ELRQVGAQIVLGNTFHLWLRPGVEVIARHGGLHGFMGWDGPILTDSGGFQVFSLGALR
                     KIGEEGVTFASPINGDRLLLTPEESMRVQRALDSDIAMVFDDCTAYPATEKQAEKSMS
                     LSLRWAERSKAAFQGSANALFGIVQGGLYEDLREASLDGLTRLGFDGYAVGGLAVGEP
                     EEARARVLAHTAPRLPADQPRYLMGMGTPEDLIAAVLAGMDMFDCVLPTRNARNGWLY
                     TRRGDVKIRNAKYRADERPLDELCRCYCCSNFSRAYLHHLQKSNEILGARLNTLHNLH
                     YYQALMRELREAIEGARLAPTAARLLEERGLLL"
     gene            12982..13302
                     /gene="yajC"
                     /locus_tag="AC16O9_13525"
     CDS             12982..13302
                     /gene="yajC"
                     /locus_tag="AC16O9_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR6517669.1"
                     /translation="MISNAYAQAAGGGDPGFVGLLPIVLMFVLLYFLMIRPQMKRAKE
                     HKQMVEALQKGDEVITSGGVVGRISKMGDAYVTLEIAPNTEISVQRAAVQTLLPKGTM
                     KTIQ"
     gene            13310..15169
                     /gene="secD"
                     /locus_tag="AC16O9_13530"
     CDS             13310..15169
                     /gene="secD"
                     /locus_tag="AC16O9_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR6517670.1"
                     /translation="MNRFPAWKYALVALCVAVALFYTLPNFFGESPAIQVSSLRATLK
                     ADTALLQRVESALSQATIRYTGALLDAGSVRVRFADTDTQLKAKDLVDRSLNPDPQSP
                     DYVVALNLLPSSPRWLTALGALPMYLGLDLRGGVHFLLQVDMQAALTKRLESLTGDIR
                     SLLREKSIRHGGVTREGQTLRVRFRDAESRERARAVLDSGLGDLALAAREQANEFLLV
                     ATLKPDAAKRTQEYALKQNITTLHNRINELGVAEPVIQQQGADRVVVQLPGVQDTAKA
                     KDILGRTATLEIRMVDEEKMTPDLLAAAARGDVPFGTEYYVERNGAPLLVRKQVVLTG
                     DRLTDAQPGFDSQTNEPAVHLTLDGAGARIFKEVTRENVGKRMAILLIERGKGEVVTA
                     PVIRTEIGGGRVQISGRMSTVEANDIALLLRAGSLAAPMEIIEERTVGPSLGRDNIEK
                     GFNATRWGFVAIVTFMSVYYVAFGVISSLALGINLLFLVALLSLLQATLTLPGIAAIA
                     LTLGMAIDANVLINERIREELRGGATPQAAIAAGYERAWGTILDSNVTTLIAGLALLI
                     FGSGPVRGFAVVHCLGILTSIFSAVVVSRAMVNLFYGHRRKVQRLMIGDTAWK"
     gene            15214..16119
                     /gene="secF"
                     /locus_tag="AC16O9_13535"
     CDS             15214..16119
                     /gene="secF"
                     /locus_tag="AC16O9_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR6517671.1"
                     /translation="MRHALVFNVISVVTFLLAVFFLATRGLNFSIEFTGGTLLEVSYQ
                     QSADVDAVRQQLEQAGYKAEVQNFGSSRDVLIRMHVEKSQSSADLSEKVLGVLRTGNA
                     AVELRRVEYVGPQVGEELAEYGALALLVTAVGIVLYLWMRFEWRFGLSAIIANLHDVI
                     IILGFFSFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRVREMFRKLRKASVPEVIDTA
                     ITGTISRTIITHGSTQAMVTTMLIFGGPALHYFALALTIGILFGIYSSVLVASPLLMW
                     LGVDRAQFVKPLKKTGRDEHEGAVV"
     gene            complement(16120..17484)
                     /gene="purB"
                     /locus_tag="AC16O9_13540"
     CDS             complement(16120..17484)
                     /gene="purB"
                     /locus_tag="AC16O9_13540"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR6517672.1"
                     /translation="MPSPLAALSPLDGRYERSTEALRPYFSEAALIRYRVLVELEWLK
                     ALAAERRLRELKPFSRATRLALDRLVAGFGEKDATHIKNIEAETNHDVKAIEYWLKGR
                     LAKNAEVQRSLEFIHFACTSEDINNLAYALMLAHSRDRVLLPRLDQLIVALRGLARRH
                     AARPMLSRTHGQPASPSTLGKEMANFAHRLVRARAAIERVALQGKLNGAVGNYNAHAA
                     AYPGFPWEAFCRRFVERLGLQFNPFTTQIEPHDSYAELFDAYARANTVLLDLDRDLWG
                     YVSLGYFRQKPKKGEVGSSTMPHKVNPIDFENSEGNVGVANALLRHLADKLPVSRWQR
                     DLSDSTALRNAGVALGHTLLAYGACVRGLAKLEADPARMAEDLEANWEVLAEPVQQVM
                     RKHGVPDAYEKLKALTRGRRLDRRQLAAFVRTLPIPAAAKKRLLALTPAAYTGLAADL
                     ARRV"
     gene            17505..18074
                     /locus_tag="AC16O9_13545"
     CDS             17505..18074
                     /locus_tag="AC16O9_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR6517673.1"
                     /translation="MLEALIREARRCRVCAPHLPLGPRPVFMVGAGARLLVVGQAPGR
                     RVHETGIPWNDPSGEQLRAWLRMDRARFYDPTRVAILPTGLCYPGSGARGDLPPRPEC
                     APLWQPRFRAALPGVHLTLLIGLHAQRYYLGARRKRTLAQTVRAYAEYLPEFFPLPHP
                     SPRNRIWMRNNPWFARQVVPALRRRLRTL"
     gene            18087..18698
                     /locus_tag="AC16O9_13550"
     CDS             18087..18698
                     /locus_tag="AC16O9_13550"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6517674.1"
                     /translation="MKLLSAPASPFARKVRIVLAEKRIDCDLERVDVQPQDNPVNAHN
                     PLGKIPTLILDDGTAIYDSRVIIEFLDNMTANSRLIPQDNRERIAVRRWEALADGVLD
                     AGLLIRYESIRPPSEQSASWTEKQAARMRRGLAQLQADLGGRSWCQGERYTLADIALG
                     CCVGWIGFRKPAGIDWRADYKDLAKHYDKLMERPAFADTVPAA"
     gene            complement(18695..19753)
                     /gene="mnmA"
                     /locus_tag="AC16O9_13555"
     CDS             complement(18695..19753)
                     /gene="mnmA"
                     /locus_tag="AC16O9_13555"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR6517675.1"
                     /translation="MREKVVVGMSGGVDSSVAALLLKRAGYDVVGLFMKNWEDDDDDA
                     YCSTREDLIDAAAAADVIGVPLEAVNFAAEYRERVFASFLAEYSAGRTPNPDVLCNAE
                     IKFRAFLDHALQLGAHKIATGHYARLRTGANGVELLRGADAAKDQSYFLHRLTQAQLA
                     RVMFPVGDLTKTEVRRLAAQAGLPNHAKKDSTGICFIGERPFREFLNRYLPRTPGPMV
                     TPEGRRVGEHIGLAFYTIGQRKGIGLGGAGEPWYVAEKRMHANELVVVQGHDHPLLMR
                     SSLDGLDSSWVGPAPAPGSMHGAKTRYRQADAQCTVSRIADSEVAVSFPRPQRAITPG
                     QSVVLYDGEVCLGGAVIA"
     gene            complement(19746..20198)
                     /locus_tag="AC16O9_13560"
     CDS             complement(19746..20198)
                     /locus_tag="AC16O9_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6517676.1"
                     /translation="MSRWRPSVTVAALIERDGRFLFVEEEANGRRVLNQPAGHLDPGE
                     SLAQAVAREVLEETAHRFTPTGLVGIYRWRYDKEDVTFLRFAFNGRVDGVQDGRQLDK
                     EIIALAWLTPAELAARRTEHRSPLVQQCVDDYLAGRSFPLEVLSKPYA"
     gene            complement(20195..21409)
                     /locus_tag="AC16O9_13565"
     CDS             complement(20195..21409)
                     /locus_tag="AC16O9_13565"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6517677.1"
                     /translation="MPDISRRARHLGTENAFVVLAEVNALARQGKDIISFCIGQPDFP
                     APEHVQEAAIRAIRGGKHGYTPSAGIDELRAAAATYMARMRGGLPIRAEDVVVGAGAK
                     PFIAYAILSTTDYNAGDEVIYPNPGFPIYESQILANGAKPVPIHLHEAREFAFDPQEL
                     ERLITPKTRLLILNYPHNPTGGTLTKKDLEAIAAILRKHPQVWVYADEIYSRLCYAGE
                     FFSIAQLPEMYERTIISDGASKTWAMTGWRIGFTSNPSLAPVFTRWITNTESCASQIS
                     QWAAVEAITGPQDAAEKMRASFLERRDLIVGLLNQVPGVACRNPGGAFYAWPNVTEAC
                     KRIGATDSEEFRKRLLNEAGVAVLADIHFGARVPGEGQHVRFSYAASRQAIEQGVARM
                     AEFIKKNTRKAA"
     gene            21456..22421
                     /gene="corA"
                     /locus_tag="AC16O9_13570"
     CDS             21456..22421
                     /gene="corA"
                     /locus_tag="AC16O9_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR6517678.1"
                     /translation="MLVNCVVYQDGKKLADIQKREIRAYLQRPECFVWVALRDPDAAE
                     LAEMQDEFDLHELAVEDARHGNQRPKLEEYGDSLFAVLHTVEVAGDELKVGEVDVFVG
                     RNYVLSARQKTERGFQDVRARCEREPELLRHGSGYVLYALMDAVVDRYFPVLAAVESQ
                     LEKLEAQIFTGKSPRENIESLYYVKQKLMTLKHAAGPLPETSSRLFGGRVPPACAGLG
                     EYFRDVYDHVVRLNQSIDTLRDTVTTAIQVNLAMITIGDSEVTKRLAAYAALVAVPTM
                     IAGIYGMNFANMPELGWSFGYPLSIAIMAAIDGYLFYRFRKAAWL"
     gene            complement(22442..23791)
                     /gene="ffh"
                     /locus_tag="AC16O9_13575"
     CDS             complement(22442..23791)
                     /gene="ffh"
                     /locus_tag="AC16O9_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6517679.1"
                     /translation="MLENLTARLARIVKTIRGEARLTEANVQDALREVRVALLEADVA
                     LPVVRDFVAAVKQKALGEEVVGSLTPGQALIGVVHRELAALMGGAHAGLDLAAQPPAV
                     ILMAGLQGSGKTTTAGKLARLLLGQKKKVLLVSCDVYRPAAIEQLAAVAAQAGADVFP
                     SAVGEAPQDIARRALEHAKTRYYDVLIIDTAGRLGIDEAMMQEIAALHGQVKPVETLF
                     VVDAMQGQDAVNVARAFGERLPLTGVVLTKLDGDARGGAALSVRQVTGKPIKFAGVGE
                     KLDALEPFHPERMASRVLGMGDILSLVEEAHKQVDLEQAQRVAEKLKKGKGFDLEDFK
                     QQLLQMQKMGGMASMLDKLPAQLAGQINPAQLQDDRQLRRTAGVINAMTPQERAHPEL
                     IKASRKRRIAAGAGVPVQEVNRLLNQFEQMQKMMKMMKGGNMARMMRNLKGALPGMR"
     gene            23810..24676
                     /locus_tag="AC16O9_13580"
     CDS             23810..24676
                     /locus_tag="AC16O9_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR6517680.1"
                     /translation="MPDIVIHAATAAAYAGLAWHFWRTRWRAQPAGTVPLAGTRPWER
                     VALLAPLALHAYLLYAELFAPSELRFGFAQALSTMLWLAVLIYWFESLFFSLEGMQPP
                     VLALAALAAPLPALFPGRASVLAASLEFKVHLAMAMLAYSLFTIAILHALLMALLERR
                     LHGSAAARAALPAEGAGLLSGPLGALPPLLTLERLLFRMIAAAFVLLTLTLASGIVFS
                     ESMFGRALRFDHKTLFAVLSWATFALLLAGRYLYGWRGRIALRWTLSGFLMLLLAYIG
                     SRFVLEVVLGRG"
     gene            24678..25973
                     /locus_tag="AC16O9_13585"
     CDS             24678..25973
                     /locus_tag="AC16O9_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR6517681.1"
                     /translation="MEDLPIGWSFGALAVLLVLSGFFSIAETSMMALNRYRLRHLVAQ
                     GHGGAKRAAELLKRTDTLLGAILIGNNLVNAAAATLAGLIAVRLMGQDKLAYAVSTVA
                     ISFLLIVFAEITPKVIGATYPERIALPFAYVLGPLRSLLRPGVWFVNLFVRPLLLLAG
                     VRPGESTEVQKLTPAEIRTLVLESSSFMPKKHVSILLNLFDLGSITVQDIMLPRARIE
                     SVKLDDDTAVIARQLGTSYHMRLPVFRDSRGDVLGVLHLRRVLAPLMAGTLDKAQIEA
                     LLAPPYFVPATTPVFAQMQYFQENEERIALVVDEYGELMGLVTLEDIIEEIIGKFTTS
                     LPAAAHALAWAADGSATVDGATPVREVNRALGLDLPTEGPKTLNGLILEHLRDIPEAD
                     VSIKVGEVPLEIVHAQGRSVKTVRIFRPPAPPPGLAEAP"
     gene            26114..27337
                     /locus_tag="AC16O9_13590"
     CDS             26114..27337
                     /locus_tag="AC16O9_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6517682.1"
                     /translation="MATAAAKAAGPKEFNFLWEGRDKGGKVIRGELRSVSEAAVNATL
                     RRQGIIVNKVKKQKYGSGGKVTDKDITLFTRQLATMMKAGVPLLQSFDIVSKGASNPA
                     VARLLLEVKTEVETGNSLAAAFRKYPLHFDALFCNLVAAGEQAGILETLLDRLATYKE
                     KILAIKSKIKSALFYPIAIIVVAFVITAVIMIFVIPAFKEVFTNFGADLPAPTLMVMA
                     ISDWFVANWYIIFPAVIGGVIGFLEAWKRSVPVQIFMDRLMLKLPVFGDLVLKSTIAR
                     WTRTLATMFAAGVPLVEALDSVGGASGNHVYLTATKKIQTEVSTGTSLTMAMQNSGVF
                     PNMVLQMCSIGEETGALDAMLGKVADFYEAEVDDAVEALSSLMEPMIMVVLGTLIGGM
                     VIAMYLPIFKMGQAV"
     gene            27338..28210
                     /locus_tag="AC16O9_13595"
     CDS             27338..28210
                     /locus_tag="AC16O9_13595"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR6517683.1"
                     /translation="MAAFGWLAQPAVLPWVALALGLCIGSFLNVVIHRLPKMMEREWR
                     EQCAELAGTPAPAAASAHGRYNLLVPRSACPACGHRITALQNIPIVSWLALRGKCAAC
                     GTRIAARYPFVELVAGLIGAYAAWRYGATLAALGAAAFGWALLALAVIDLDTQLLPDD
                     ITLPLLWAGLLVNFGAVFAPLRTAVIGAVAGYVSLWLVYWAFKLATGKEGMGHGDFKL
                     LAAIGAWLGWQKLPLVILLSSVVGAAVGIALIVLARHGREKPIPFGPYLAAAGLIALF
                     WGDVITRRWLPILG"
     gene            28210..28818
                     /gene="coaE"
                     /locus_tag="AC16O9_13600"
     CDS             28210..28818
                     /gene="coaE"
                     /locus_tag="AC16O9_13600"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434090.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR6517684.1"
                     /translation="MAFIVGLTGGIGSGKSAAADEFARLGAAVVDTDAIAHELTRAGG
                     AAIAGVRRVLGDEFVDASGAMDRAKVRSLVFSDRAARARLEGLLHPMIRAESERRIGA
                     ATAPYVVHVVPLLVESPDYRQRVQRVLVVDCSPQVQKARVGQRSGLAIDEVERIIDAQ
                     IPRETRLAAADDVIDNSGSLDALHKQVRALHRRYLDMARTPA"
     gene            28874..29632
                     /gene="zapD"
                     /locus_tag="AC16O9_13605"
     CDS             28874..29632
                     /gene="zapD"
                     /locus_tag="AC16O9_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HIR6517685.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERSRHFIARTDPLDHHVALLTL
                     FEILEVSSRADLKSDLLQELERQKQVLLSFRNNPDISEEALSSVLRDIEQAAAALFSM
                     TGKIGQYLRENDWLMSIKQRTGIPGGACEFDLPSYHFWLHRPAEERIGQLAAWTGPLF
                     PLRDGSAIILRILRESGKPQRLSAPLGTFQQMLGGKTAQMLRVRLDPAANCVPEISAN
                     KYVLNIRFLTQASEDPKPRAADWDVGFELTFCNL"
     gene            29659..29799
                     /locus_tag="AC16O9_13610"
     CDS             29659..29799
                     /locus_tag="AC16O9_13610"
                     /inference="COORDINATES: protein motif:HMM:NF015821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR6517686.1"
                     /translation="MPAPFAAANPWRPFCSERCRLIDLGNWAAESYRVPESGPRPPDE
                     TD"
     gene            complement(29796..30707)
                     /locus_tag="AC16O9_13615"
     CDS             complement(29796..30707)
                     /locus_tag="AC16O9_13615"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HIR6517687.1"
                     /translation="MALEVAAAVIQRADGAFLLAQRPAGKVYAGYWEFPGGKVEPGEA
                     AERALARELREELGIEACTSFPWLTREYTYPHASVRLNFFRVTAWKGDPQPKEQQAIA
                     WQRFGTPLAAPMLPANAPVLAALALPHEYAITDAQSQGTRVMLSRIGVRLKQGLKLIQ
                     VRDRELAGREAFVRDVVRLAHRHGARVLVSGAPAPEADGVHYTAAQLMRLESRPREGL
                     AAASCHTPEELARAAALELDFAVLGAVEPTRSHPGARLLGWDGFARIARGASLPVYAI
                     GGLRRRDLEAAWRAGAHGVAMIGGAWA"
     gene            complement(30709..>31249)
                     /locus_tag="AC16O9_13620"
     CDS             complement(30709..>31249)
                     /locus_tag="AC16O9_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6517688.1"
                     /translation="KQGLRLIEVEKTDLVDLAAITDQVAGRDERFLVFCDDLTFHGAE
                     DGYIALKVALDGSISTTSENVLIYATSNRRHLMPEYMAENLETKYVGDEIHPGETVEE
                     KISLSERFGVWVSFYPFDQDEYLEIVAHWLKTLGCGPKAAAAAREEALQWSLQRGSRS
                     GRIAYQFARDWAGKHAGAK"
BASE COUNT         4845 a        10874 c        10452 g         5078 t
ORIGIN      
        1 ccgctgctcg agcgcgtacg ccaccccgtg gccgtcgacc ccgacgcgcc gctcgccgcc
       61 gaggccgagc ggcgcggctg gccggtgatc tcgctgcgct agaatcgcgg cctcgatgat
      121 caagcgcttc atccgccgcg tcctgggcct tccttcgcat gcgccgcagc gtgtgccgcg
      181 cgcgcggcac ccgctgcggc gcgcggcgct cagccacgcc gcgctcaagg tctgcgacgt
      241 gctgcgcgat gcgggtcatt ccgccagcgt ggtcggcggc gccgtgcgcg acctgctgct
      301 cgacatcgtg cccaaggatt acgacgtcgc caccgatgcg cgccccgagc aggtgaagcc
      361 gttgtttcgc cgcgccctca tcatcggccg gcgcttccgc ctggtgcacg tgatgctggg
      421 gccggaaacc gtggaggtgt cgacgttccg cgcggccgac gccgggacca cggaaaagga
      481 cgagcacggg cgcgtactgc gtgacaacgt gtttggcagc caggaggagg atgcgcgtcg
      541 gcgcgatttc acggtcaacg cgctttactt tgatcccgcc accgaggaga tcatcgacta
      601 tcacggcggc cttgcagacc tgcgccggca cgtgctgcgg atgatcggcg acccggagac
      661 gcgctatcgc gaggacccgg ttcgcatgct gcgcgcagtg cgcttggcgg caaagctggg
      721 actgacgatc gaggcgggga cgcgcgcgcc gatccgtagg ctcgcccccc tcatcgagca
      781 cgtgccgccg gcgcgcgtgt tcgacgagat gctgaagctg ctgctctcgg gccacgccag
      841 cgcctgcgtg cgccagctgc gcgaagaggg gctctccaag ggcgtgctgc cgctgctcga
      901 cgtgatccta gagcaaccgc tcggcgagcg cttcgtcacg ctggccctgg cgcagaccga
      961 cgagcgcgtg cgcaccgacc ggtcggtctc gcccgcgttc ctgttcgccg ccctgctctg
     1021 gcacgaggtg ctggcggcgt ggaaggcgcg gctggcgaaa ggcgagcgcc cgctccccgc
     1081 gctggaggcg gccatggaca gggtgctgga cgcacaatgc gaaaagctcg cgcttacccg
     1141 caagctcacg gcgacgatgc gcgagatctg ggcgatgcag ccgcggttcg agcagcgcgg
     1201 gggccagcgc ccgtcgcggc tgctggaagc accgcgcttt cgcatggggt acgactttct
     1261 cgcgctgcgc gcggcgagcg gcgaagtaag cgccgagctc gaagcctggt ggcgcgcctt
     1321 ccagcaggcc gacgccgatt cgcgccgcgc gatgctgctg cccgacacgg gaccgcgcaa
     1381 gaagcgccgg cgtcggcgcc ggcgccagga cgccgccgcc accaccgagc aggcgtgatc
     1441 acggcgtaca tcgggctcgg cgccaaccag ggcgaaccgc gcgcgcagat cgaccaggcg
     1501 ttcgccgagc tcgcggcgct ccccgcaacg cggctcgccg cccgttcacc gctataccgc
     1561 agcgcgccgc tcgatgcgcc cgggcagccg gactacgtca acgcggtcgc ggccgtcgat
     1621 accgatctgt cggcgccgca gctgctcgac gcgctgcagg ccatcgaggc gcggcacggc
     1681 cgcgagcgcc cgcaccgcaa cgcgccgcgc acgctggacc tcgatctgct gctgttcggc
     1741 gagaccgcgt tcgcctcccc aaccttgacg ctgccgcacc cgcgcatgca cgagcgcgcc
     1801 ttcgtgctgc ggccgctgct ggatcttgac ccgcgcgcgg aggtgccggg tcgcggctca
     1861 gcgcgggcgc tgctgcgcgc ctgcgcggcg cagcgcatcg agcgcatcgg gtagatggag
     1921 cgctaccgtt acatcgccgt cgagggtccc atcggctcag gcaagacaag cctcgcccgg
     1981 cgcctcgccg cgcggctggg cgccgatctg ctgctcgagc agcctgcgga gaacccgttc
     2041 ctcgcccgct tttacgagga catggcgcgc tacgccctgc cgacgcagct gtttttcctg
     2101 ttccagcgcg cgcgccagct cgagcccctc gcccagcccg acatgttcgg gcgcgctacg
     2161 gtggccgatt tcctcatcga caaggatccg ctgtttgcac gcatcacgct ctccggcgac
     2221 gaactgacgc tgtaccagcg catctacgag gcgctgcggc cgcaggcgcc ggcgccggac
     2281 ctggtgatct acctgcaggc gcagccccag gtgctcatcg agcgcgtgcg acgccgcgcc
     2341 gccgcctacg agcgcggcat cggcgaggag tacctcgcgc tgctcgccga aggctacgcg
     2401 cgcttttttt accactacgc cgcggcgccg gtgctgatcg tcaactcgga gaacctgaac
     2461 ttcgtggagc gcgacgccga tttcgacctg ctcgtgtcgc gcatgcgcgc catgaaaagc
     2521 cgccgcgagt acttcaacct gggctgagcg ccgcgcctac gccggatacg ggtcgaaggt
     2581 gatggactcc accgggcagc ccatcgacgc cgcgatcctg tccatcgcgt cgtcggtgag
     2641 cgccaccgga ctgaaatcct tgtcgccgcc cttagcggag atgaaggcct tcgcggcgtc
     2701 ctcggtgttc caggtcacga ccgggtcggg gctgacccct gcgacctgcc ggcagatcgg
     2761 gaagccgtcg gacttgtgga cgatcgcata catgctgccc ccttcgcatt gccttcgtcc
     2821 cgctgcgatg agtcccttat aaccgattcc ctccggcccc gccaagcccg cggcgctgcg
     2881 cgacgttgac gattgtgcgc ggcccgccga gtcgacgcga aaatatttcc gcacgagcaa
     2941 tcgtgtgagc gcgtgccgtg cgcgcaatgc ggcgcgtcaa gtcaacgcca tgatgcgcga
     3001 cgctgcagcg cgcgacaccg ttgcgccaat caccatgtgc ccgcgggccc gaaacgcggc
     3061 ccggtgacat ctgtcgaaat ctcaacagtt tctccgtgaa gtagttcacg cggtctaagg
     3121 taatccctcg tcataccctc aggattcgcc tgatttgcat agtgattgtc aaacagcgtt
     3181 aaatggaata cgggaggtcc ggacgcgcat agcgcgtccg ggccggggca accggccccc
     3241 cggcaagcgg gggctttttc aactggggaa ggagaagcac atgagcaaca tgttcgatgc
     3301 agtcggcctg cgcgcgccct gcacgcgccg ctacacggag ctggagtacg agttcgagcc
     3361 agtaagcggg acgctgtggg gcttcatgaa gcagcgcggc aacgcctgct tcaacctcgg
     3421 catcctgaag gacatccgcg accacgacga agcgctctcc gccaatggtg gctgcgtcga
     3481 cttcgaaggc acgctgcagc gcgtgaactg gtacgtggcg gcctcgcgcg tgcccaaggt
     3541 gttcaatctc ggcggcgacc tcgcgctgtt cctgctgctg atcaaggcgc gtgaccgcga
     3601 cgcgctcgcg cactacgcgc gcctttgcat cgacaacgtc tacgcgcgca tcaccaacta
     3661 cggctgcccg aacgtaacca cgatttcgct ggtgcagggc gacgcccttg gcggcggctt
     3721 cgagaccgca ctgtcctcgg acgtgatcat cgccgaggag tcggcgcaga tgggcttgcc
     3781 ggaaatcatc ttcaacctgt tccccggcat gggcgcctac agcctgctcg cgcgccgcat
     3841 cggcatgcgc gccgcggagg acctgattct gtccggacgc gtgctctcgg ccgccaagct
     3901 gcacgaaatg ggcgtggtcg acgtactggc gaaggacggc gagggcgaga ccgcggtgca
     3961 cgcgtggatc gccgccaacg ccaagcgccg caacgggctg caaggcgtgc tgcgctcgcg
     4021 ccagcacgtc ttcccgatca cgcgcgccga gctcgacgct atcggtgata cctgggtgga
     4081 ttgcgcgctg cgtctcgagg accgtgacct gcgcatgatg ggtcgcgtgg tgcgtgcgca
     4141 gatgcggcgc ctggaggaag gcggcatcac cgacatcatc gccgaggccg aggcgcttgc
     4201 cgccgcgggc tgagcgccta tgccgggccg agcggcccgc cggtcctgaa gacctggctg
     4261 aactggtgaa agttcacccg tgtcgagcgg atgatcgccg cctgcgcggc ggggtccagg
     4321 atcgtcttga ccaggcggtt gaggctcgcc atgtggtcga ggtccatggt cgcgtgcgaa
     4381 gaaaggaact tgaagccgcc tgccgccaca tcgcgcccga gcttgcgcgc aatcgcgtcg
     4441 gacacgcgcc ccccgtacac cgacgcaatc acctcgagca cgtagagcat gccgagcacg
     4501 gagcacggat gcacgtgttc cgccgcgtag tagttgaacg cgatcatcgc ctgcaccgga
     4561 gcactcggca gcccggcacg cgttgcggag acatcgcccc ccattgcctc gatgtcttcc
     4621 agcacccatc cctcgtgtcc cttctcctct tcgatgcgcc cgtagagttc gaagcgcacg
     4681 tcgcgaaagt cgtcgccgca gcgcgccacg gccgccgcca tcaccgggca gaaatgccac
     4741 acgatgtggt agaggtcgtg caggaaggcg aggtattcgg gcaacgtcag cccgtggtgg
     4801 accatcgcgt gcacgcgcgg ctcggcctcg agggcgcggc gactcgcatc ggtgctttcc
     4861 acgagctgga tgaagaagga catcgcgaaa ttgtagcgcc agtcccgatc tttcgaccgc
     4921 ggcaattgcg aagacgccga tggacccgat caggctctac ctgctgatac tgccggtatt
     4981 cctcgccgcc atcgcggtcg aggcgctcta ttaccgtgtc gcactgcacc ggccctacgg
     5041 ctggaaggtc acggtctcca acctcgccgt ggccatcggc cgcctcggga ccgacgcctt
     5101 caccaaggcc ctggttgcgg cagtgtacgt tgcggcctat cagctgcggc cctttgacat
     5161 tcccctggac cgctgggaaa gctgggtagc gctgtttctc gccgtcgagc tggcctatta
     5221 ctggctgcac cgcttcagcc acgagatccg ctggatgtgg gcgcaacaca gcgtgcacca
     5281 ctccgcgcgc cagatcacgc tctcggtcgc ctaccgcctg ggctggacgc agttctttgc
     5341 cgggccgtgg ctgttcctgg tgccggtatg ctggatcggt ttcgatccgc gtgcggtagt
     5401 gctgatgtac gcgatcaatc tgctgtacca gttctggctg cataccgagg tcgtgcccaa
     5461 gcttgggccg ctcgaagcgg ttctcaatac gccgtcgctg caccgtgtgc atcacgccgt
     5521 cgagcccgaa tacctggacc gcaactacgg tggcgtgctg atcatctggg accggctgtt
     5581 cggcacctat gccaaggagc gcgatggcgc gccccgcacg tacgggctgg tgcgccagat
     5641 cgacacgctc aacccggtaa agatcgcgtt cgccgagtgg gtggcgatgt tcagggaatt
     5701 gcgtacggcg cgcagtctgc gcgaggcgct cggccttgcc ttcggcccgc cggggtggaa
     5761 agccgatggc tcgggccaaa ccacccggat gatccgccgc gccgcgggca ttccggagaa
     5821 ttcgcgcgcc cgcgcgccgg tcgcggcgct ggccgcggag cgcgacgcgc cccgttagcg
     5881 cgcccgtcga cgttactcag gaatccggcg gccgcagcca cgccaccagc cgcggccaca
     5941 aggcgagtcc cgccctgcgg ctgaagaagc cgaaatgccc gatcttgcgc acgccagcgt
     6001 cagcgggcgt gaagagaagg tgctgcggcg acagccttgg atagtaggca agcagtcgcc
     6061 gcgtgccttc ggccgtggca aacgcgtcat cgctgatccg tatgacgagc gcgggtcggt
     6121 ccagcgcggc gcaacggtcg aggaggcgcc gcgtccgctc ggcggccgga ctggcgccgg
     6181 cgggtcgcag gtcgggcgag tggcgcgcag cccattgcag cgccacaccc gcgggcatgt
     6241 cgtcgcccag acccaggcgg cgcgcaggaa aatagcctac caggcgagtg agcgctggca
     6301 tcagtccgtg ccagagcatg gccatcggca atcggtagcg cagaagataa tcgccgtagt
     6361 agcccgtatg cgcgccgatc aggatcatgc gcgcctgctc tgcagtattg tgcgctccgc
     6421 caaagagcag cgcagcgacc gaatgggcga taccgatcag ttccgtgcgc gggaagcgtt
     6481 cgcgcaacca gccaatcgcc gcgctgcagt catgttccgc ccagtcctcg atcaccgctt
     6541 ggaaaccccg caatgcgggt ggtcgcgaca gtccgatgcc ccggaaatcg taggtgagca
     6601 ccggtatgcc cgagcgcgcg agaaagcgag cgaaccgccg gtagctcagt gccgggatcc
     6661 cgccgccgcc gtgcaggact gcggcgcggg gcggcgccgc ttccgtgggc acagagtaaa
     6721 ggatcgcgcc caactcatag ccgtccgcgg cgcggatccg cagcttccgc tcgtcaaccg
     6781 ccggctcgag tcggtcgctg ccagccaatt ttcacaccgc aaggtcttgg acgaggagcc
     6841 ggaattgtat aggacagcgg ctcgccgccg cgcgacggcg gtcgacgcgg cgctagcccg
     6901 ccgagcggct gcgcttttcc cgtatatagc gctccagggc ggagcgcccc tgagcgaact
     6961 cctcgttcag cgagcgtacc agagacgatg attgcagccg cagctcggcg tcggagtact
     7021 tgcccagctt cgaacacagc cacgtcaggc gctcggtgcc gatgctcgcg gcagacccct
     7081 tcatggcatg caagtgcgcc cggaactcgc cgaagttgcg cgccgccacc gcggcttcca
     7141 tctttgccag aagcgactgg ctgtcggcga cgaaaacgcc gacaagccgc tcgaggaaat
     7201 caggcgtcga gcccagttct tcgaggcgcg ccagggtctc gatgtttacc ggctcggcgc
     7261 cggtcggcgc cgccacggcc acggccgcag gcgcggcgcg cgccgcctcc tgtggcttgc
     7321 cccccgtcaa gctgcgcatc tcctcgagca ggcgcaaggc ctcaaccggc ttgggcacga
     7381 aactatcggc ccccgcctcc aggcactcgc gcttcgcctc cagcgtaacg tccgccgaga
     7441 gcaggatcac cgggagccgc tcccggccgc gcgtcatcag gcggatcgcc tgcagggttt
     7501 ccaggccgct cagacccggc atgttgcgat cgagcagcac gatgtcgtag tggccagtct
     7561 ccagggcgtc gagcgcttgg tcgccgtcgg cgaccagcga taccgcgtgc ccggcgcgct
     7621 cgaggatgcg acccagcacc tcgcggttgg tgggattgtc gtccgccacc agcaccgaca
     7681 ggcccctggc gccctggccg cgcttggcgt agtcctgcag ccgcaccacg ccctcgcgca
     7741 attcgtgcga ggaggagatc gcgtgcagca cgttgaacag ctggcgcttc tcgaacggca
     7801 gctcgagcac ggcactgaat ccggccgaca gcgccgaaaa gcgctgcgcc tcggcctcgc
     7861 gcggcacagc cagcactgcc ggcggcgccg gctcgggcgc ggcgcgccgc aggcgccgcg
     7921 ccagctgcgt gtcgccgccc tcggagtaca tcagcacgct gtgatagggc ttggcgaggg
     7981 cgatgtcggt gatcaggcgg tcggcccctt cctccacgct ggcggcgcgc acgcccatgg
     8041 cgccccaggc ttcgagggcc tcctcgatgg gctgcgcctg gtgcgcgggc atgccgacca
     8101 gcaggatccg cgcccccgcg agttcgccgg caccggcact cgggcgctcg ggctgcttct
     8161 cataagtgat gtcgaaccag aacgtgctgc ccagaccaac ggcgctttcc agcccgatct
     8221 tgccgcccat caactcgacc aactgcttgg cgatggtagt gcccaagccc gtgccgccaa
     8281 agcgccgcgt ggtggactgg tccgcctgcg tgaagctctc gaaaatggtc cgctgcgcct
     8341 cctgtgcgat gccgataccg gtatcgcgca ccgaaaactt gagacgcacg ctggtttcgg
     8401 tttcctcctg cagcgacacg tgcacggtga cgctgccacg ctcggtgaac ttgaccgcgt
     8461 tgcccgccag attgatcagg acctggcgca gatgatgcgc gtcgccgcgc agcgcgggcg
     8521 gtacctccgg catgatcgag accacgaact cgacaccctt ggccttcgcc tgcgcctgca
     8581 ggatgcggct ggtgctgttg accagcgcgt gcaggtcgaa atccgtgcgc tccagtacga
     8641 gcttgccggc ctcgatcttg gagaagtcga gcacgtcctc caccagcccc agcattatct
     8701 ggctcgaacc gcgcagcgtc tgcagcatgt ccgcctgctc gcgcgtcagc gcggtgtcgc
     8761 gcatcaggtc ggccatgccg atgatggcgt tgagcggcgt gcgcatctcg tggctgacca
     8821 ccgaaacgaa tcggcgcttg gcttcgttgg cagcctcggc gcgtgcgacg gcgtcgaaca
     8881 tgcgcctcac cagcgtcagc acgtacaggc tcagcgcaac gaacccgacc atcatgcccg
     8941 tgccaacgct ggtacgcgcc tgccagtatt cgctcacgtt cagcgcgatc gcgaagccga
     9001 gcatgctcag catcatcgaa ataacgaggt agcgcgcgcc gtagcggaaa ccgttgccga
     9061 tcgtgaacca cagatagacg aggaacagcg gaatgcccaa ctcctggaaa tacgcgatac
     9121 accagctggt ggcggcggtg tcgagaacca tgccggcgat gcgacgcgcc accgacaccc
     9181 caggagaggc gagaataccg ccgaaaatga cggtcgataa ggcgaggaag ccgagccaaa
     9241 ccacgatatg cggctcgggc aggccctcgg catgtcgctg caggatctcg ggcagcaggt
     9301 aaaggcccag cagcaccccg accaccagac gcacgatcgc ctgttcgtgc tcagtgtcgg
     9361 cacgccccgc gagccgtacg cgcagccaac gccaccagac cgcacccggc agcttcatga
     9421 gcgcttcgcc tccgggtcgc gcagctcgtc ctggatgcgc gcgaccgcgc cccgcactcc
     9481 ggaaaaggca tctaccagct gcggatcgaa gtgcttgccg cgctgcgtat cgaggaattc
     9541 gaacgcctgg tccgcggacc aggcctgctt gtaggggcgg atcgaggtca gcgcgtcgta
     9601 cacgtcggcg accgccacca cgcgcgcgca tagcgcgatg tgatcaccga ccaggccgtt
     9661 gggatagccc gagccgtcgt acttctcgtg atgaccgagc gcgatgagcg agcccatgcg
     9721 cacgtacttg gaagcgctgc ccttgaggat ctcgtggccg atgagcggat ggcggcgcat
     9781 caccttcgac tcggcctcgt cgaggcgccc cgacttgagc aggatctggt cggggatgcc
     9841 gatcttgccg atgtcgtgca gcggcgcggc gagctcgatg gattccgcct cgtcgcgctc
     9901 caggccgatg gtgtcggcga tgaggcgcga gtagcgcgcc atgcgcacca ggtggtagcc
     9961 ggtctcctcg tcgcggaact cgccggcgcg cgccaggcgc atcagcgtct ccttctctcg
    10021 gttgcgcact tcgcgcgtgg cctcgtcgac catgccctcg agcaggcgcc gccggtcctc
    10081 gagcgcgagg tgctggcggc gcagggtgag caggttgcgg cagcgcgcca tgcactcgcg
    10141 cgcgtccacc ggcttgatga ggaaatcggt gacgcctgca tcgagcgcgt cgtagcgcac
    10201 cttgcggtcg tcgtgcgccg tcaccatcat gatcggcacg tgctcgtagc cgggcagcgc
    10261 gcgcaggcgc ttggcgaact cgatgccgtt catgtccggc atcatgtagt cgatcagcac
    10321 caggtcggcc acgttgcgcg tcgcccagac cacagcgtcc acggggcgcc cgaagccttc
    10381 gacgccgacg cgctcgtcga gcgagcgcac cacctgctcg aggatggcgc ggccggtcgt
    10441 ttggtcgtcg acgaccatcg cggtattgcg cgccgccggc attacgaaaa cctgcgcctt
    10501 tgccatgcca ctttccgggt gaaaacgaaa cggcgcaagc gtacgccgcg tcgttcctat
    10561 tgtccagtta acagtctgaa gcgaaagaaa gaattcgtga tggcgcggtc gttggcccgc
    10621 gcgcgccggc tccgatcgcg gcaggcgccc cgcatgccgg cttgcagcac ttttcgcgat
    10681 ggcagcacga tgcctggtcc gccgcaccgc agccccgccc tcgtgctggt gccggaagag
    10741 agactcgaac tctcatggtg tcgccaccgg cggattttga gtccgctgcg tctaccgatt
    10801 ccgccattcc ggcgcgcggg agccgaatta tagaacccta taatgccgcg gcatgcggct
    10861 tgccgagttc gactacgcgc tgcccgaaga actgatcgcc cagcgcccgg cgcccgaacg
    10921 cacggcaagc cgcctgctgc acctcgatcc ggcgagcggc cgactgcggg acctcaaatt
    10981 cgccgacctg ccctccctgg tgggcacccg cgacacgctg gtgctgaacg acacacgcgt
    11041 gatcaaggcg cgccttgccg ggaagaaggc gagcggcggc aggatcgagc tgttcatcga
    11101 gcgcgtgctc ggagagcgcg aggcgctggc gctcatccgc tcgagccacg cgccgcgcgc
    11161 cggcaacgaa ctcgtcgtgg gcgacggcgc ccgcgtgacg gtcgttgggc gcgaggacga
    11221 cctgtaccgc gtgcgcttcg ccggagcgct gtccgcggtg ctcgagcgca gcggcgaagt
    11281 gcccctgccg ccgtacatcc ggcacgcgcc agacgcagcc gacgccgagc gctaccagac
    11341 agtgtatgcc gagcgccccg gcgcggtggc ggcgccgacc gcgggcctgc atttcgatcg
    11401 cgcgatgctg cagcgcatcg aagagcgcgg cgcgcgcatc gcgcgggtca cgctgcacgt
    11461 gggcgccgga acgttccagc cggtgcgcgt cgagaacatc gaagcgcaca gcatgcacag
    11521 ggagcggtac gacgtcccgc gcctcaccat ggacgcgatc gccgggcgcc gtgtgctggc
    11581 ggtgggcacc acgtcgcttc gcgcgctgga gagcgccgcg cttaccggac agctgcacgg
    11641 cgacaccgac ctgttcgtgc gccccggatt ccagttcctg ctcgtcgacc gcctgctcac
    11701 caactttcac ttgcccagat cgagtctgct gatgctggtg gctgcgttcg cagggctgga
    11761 gaacatacgg cgcgcctacg cacatgccat cgagcagcgc taccggttct tctcgtacgg
    11821 cgacgccatg ctcatcgagc gcgcgccgtg aaattcacgc tcagccatcg tgacggtgcc
    11881 gcgcgccgcg gccgcctcga gctcgcgcat ggagtggtgg acacgcccgc gttcatgccg
    11941 gtgggcacct acgggaccgt gaaggcgatg tcgcccgccg agctgcgcca ggtcggcgcc
    12001 cagatcgtgc tcggcaacac cttccacctg tggctgcggc ccggcgtcga ggtcatcgcc
    12061 cggcacggcg gcttgcacgg cttcatgggc tgggacggcc cgatcctcac cgattcgggc
    12121 ggcttccagg tctttagcct cggcgccctg cgcaagatcg gcgaggaagg ggtgacgttc
    12181 gcctcgccca tcaacggcga ccggctgctg ctgacacccg aggagtccat gcgcgttcag
    12241 cgcgcgctcg attccgacat cgccatggtg ttcgacgact gtacggcgta tccggccacc
    12301 gaaaagcagg ccgagaagtc gatgtctctc agcctgcgct gggccgagcg atcgaaagcg
    12361 gcgttccagg gctccgccaa cgcgctcttc ggtatcgtcc agggcgggct ctacgaggat
    12421 ctgcgcgagg catcgctcga cggcctcacc cgtctgggct tcgacgggta cgcggtcggc
    12481 ggccttgcgg tcggcgagcc cgaggaggcg cgcgcgcgcg tcctcgcgca caccgcgccc
    12541 cggctgccgg cggaccagcc gcgctacctg atgggcatgg gcacgccgga agatctgatc
    12601 gcggccgtgc tcgcgggcat ggacatgttc gactgcgtcc tgccgacgcg caatgcgagg
    12661 aacggctggc tgtacacgcg ccgcggcgac gtcaagatcc gtaacgcgaa gtaccgggcc
    12721 gacgaacggc ccctggacga gctctgtcgg tgctactgct gctcgaactt cagccgcgcc
    12781 tatctgcatc acctgcagaa gtcgaacgag atcctcggcg cccgcctcaa caccctgcac
    12841 aacctgcact actaccaggc gctgatgcgc gaactgcgcg aggccatcga gggcgcccgc
    12901 ctcgcgccga ccgccgcgcg tcttctggag gagcgcgggc tactgctata attcgcgccc
    12961 tttcccagcg agtttgcagc gatgatttcc aacgcgtacg cacaggccgc cggcggcggc
    13021 gacccggggt tcgtgggcct tctgcccatc gtcctgatgt ttgtgctgct gtacttcctc
    13081 atgatccgcc cgcagatgaa gcgcgccaag gaacacaagc agatggtcga ggcgctgcag
    13141 aagggcgatg aggtgatcac ctccggcggc gtcgtgggcc gcatcagcaa gatgggcgac
    13201 gcctacgtca cgctggagat cgcgcccaac accgagatca gcgtgcaacg ggcggcggtg
    13261 cagaccctgc tgcccaaggg cacgatgaag acgatccagt agacccggag tgaaccgctt
    13321 tccggcctgg aagtacgcgc tggtcgcgct atgcgtggcc gtcgcgctct tctacacgct
    13381 gccgaacttc ttcggcgagt cgcccgcaat ccaggtctcg agcctgcgcg ccaccttgaa
    13441 ggcggacacg gcgctcctgc aacgcgtgga aagcgccctg tcgcaggcga ctatccgcta
    13501 cacgggcgcg ctgctcgatg ccggcagcgt gcgcgtgcgc ttcgccgata ccgacacgca
    13561 gttgaaggca aaggacctcg tcgaccggtc gctcaacccc gacccgcaga gccccgacta
    13621 cgtggtggcg ctgaacctgc tgccgtcctc gccgcgctgg ctgacggcgc tcggcgccct
    13681 gcccatgtac ctcgggctcg acctgcgtgg cggcgtgcac tttctcctgc aggtggacat
    13741 gcaggcggcg ctgaccaagc gcctggagag cctgaccggg gacatccgct cgctgctgcg
    13801 cgaaaaatcg atccgccacg gcggcgtgac ccgcgaaggc cagactctgc gcgtgcgctt
    13861 ccgcgacgcc gagtcgcgcg agcgggcgcg cgccgtgctc gattcgggcc tcggcgatct
    13921 ggcgctcgcc gcgcgcgagc aggcgaacga gttcctgctc gtcgccacgc tcaagcccga
    13981 tgcggcgaag cgtacccagg agtatgcgct caagcagaac atcaccacgc tgcacaaccg
    14041 gatcaacgag ctcggcgtcg ccgagcccgt gatccagcaa cagggcgccg accgcgtcgt
    14101 cgtgcagctg cccggggtgc aggataccgc caaggcgaag gacatcctcg ggcgcaccgc
    14161 cacgctcgag atccgcatgg tcgacgagga gaagatgacg cccgacctgc tcgccgcggc
    14221 ggcacgcggc gacgtgccgt tcggcaccga gtactacgtg gaaaggaacg gcgcaccgct
    14281 gctggtgaga aaacaggtgg tgctcaccgg cgaccggctc accgacgcgc agcccggatt
    14341 cgactcgcag accaacgagc ctgcggtgca cctgacgctg gacggcgccg gcgcgcgcat
    14401 cttcaaggag gtgacgcgcg agaacgtcgg caagcgcatg gcgatcctgc tgatcgagcg
    14461 cggcaaaggc gaggtcgtca cggcacccgt gatccgcacc gagatcggcg gcggccgcgt
    14521 gcagatctcg gggcgcatga gcacggtgga ggcgaacgac atcgcgctgc tgctgcgcgc
    14581 cgggtcgctc gccgcgccca tggagatcat cgaggaacgc accgtcgggc cgagcctcgg
    14641 ccgcgacaat atcgagaagg gcttcaacgc cacgcgctgg ggcttcgttg ccatcgtcac
    14701 cttcatgtcg gtgtactacg tcgcgttcgg cgtgatctcg tcgctcgcgc tgggcatcaa
    14761 cctgctgttc ctcgtggcgc tgctgtcgct gctgcaggcg acgctcaccc tgccgggcat
    14821 cgctgccatc gccctcaccc tgggcatggc gatcgacgcc aacgtgctca tcaatgagcg
    14881 catccgcgag gaactgcgcg gcggtgccac gccgcaagct gcgatcgccg caggctacga
    14941 gcgcgcctgg ggcacgatcc ttgactcgaa cgtcaccacg ctgatcgcgg gcctggcgct
    15001 cctcatcttc ggctccgggc cggtgcgcgg cttcgcggtg gtgcactgcc tggggatcct
    15061 gacctcgatt ttttcggcgg tggtggtgtc gcgcgccatg gtcaacctgt tctatgggca
    15121 ccgtcgcaaa gtgcagcggc tgatgatcgg cgacacggcc tggaagtgag ctagcgcatg
    15181 gaattcttca agatccgccg caccattccg ttcatgcggc atgcgctcgt gttcaacgtc
    15241 atctccgtgg tcacgttcct gctcgccgtg ttcttcctcg ccacgcgcgg gctgaacttt
    15301 tccatcgagt tcaccggcgg cacgctgctc gaggtgagct accagcagtc ggcggacgtg
    15361 gacgcggtgc gccaacagct ggagcaggcg ggctacaagg ccgaggtgca gaacttcggc
    15421 tcctcgcgcg acgtgttgat ccgcatgcat gtcgagaaga gccagtccag cgccgacctg
    15481 tcggagaagg tgctgggcgt gctgcgcacc gggaacgccg ccgtggagct gcggcgcgtc
    15541 gagtacgtcg gcccgcaggt cggcgaagag cttgccgagt atggcgcgct cgccctgctg
    15601 gtcaccgcgg tgggcatcgt gctctacctg tggatgcgct tcgagtggcg cttcggcctg
    15661 tcggcgatca tcgccaacct gcacgacgtg atcatcatcc tcggcttctt ctcgttcttc
    15721 cagtgggagt tctcgctgcc ggtgctcgcc gcggtccttg ccgtgctggg ctactcggtg
    15781 aacgagtcgg tcgtggtgtt cgaccgggtg cgcgagatgt tccgcaagct gcgcaaggcg
    15841 agcgtgcccg aggtgatcga taccgccatt accgggacca tttcgcgcac catcatcacc
    15901 catggcagca cgcaggcgat ggtgacgacg atgctgatct tcggcggccc ggcgctgcat
    15961 tatttcgcgc tggcgctcac catcggcatc ctgttcggca tctactcgtc ggtgctggtc
    16021 gccagcccgc ttctcatgtg gctcggcgtg gatcgcgcgc agttcgtcaa gccgctgaag
    16081 aagaccggcc gcgacgagca cgaaggcgcg gtggtctagc tagacgcgcc gggcgaggtc
    16141 ggcggcaagg ccagtgtagg cggcgggcgt gagcgcgagc aggcgcttct tcgccgccgc
    16201 cgggatcggc agcgtgcgca caaacgccgc aagctggcgc cggtcgaggc gcctgccgcg
    16261 cgtgagcgcc ttcagcttct cgtaggcatc gggcacgccg tgcttgcgca tcacctgctg
    16321 cacgggctcg gcgagcacct cccagttcgc ctccaggtcc tcggccatgc gggccgggtc
    16381 cgcttcgagc ttcgcgagtc cgcgcacgca cgcgccgtag gcgagcagcg tgtggccgag
    16441 cgcgacgccc gcgttgcgca acgctgtgga atcggaaagg tcgcgctgcc agcgcgacac
    16501 cggcagcttg tcggcgaggt gccgtagcag cgcgttggcg acgcccacat tgccctccga
    16561 attctcgaaa tcgatcgggt tcaccttgtg cggcatggtc gaagacccga cctcgccctt
    16621 cttcggcttc tgccggaagt agcccagcga cacgtagccc cacaggtcgc gatcgagatc
    16681 gaggagcacc gtgttggcgc gcgcataggc gtcgaacagc tccgcgtacg agtcgtgcgg
    16741 ctcgatctgc gtggtgaacg ggttgaactg caggccgagg cgctcgacga agcgccggca
    16801 gaacgcctcc cacggaaagc ccgggtaagc cgcggcatgc gcgttgtagt tgcccacggc
    16861 gccgttcaac ttgccctgca gcgccacgcg ctcgatcgcc gcgcgcgccc gcaccaggcg
    16921 gtgcgcgaaa ttggccatct ccttgccgag cgtcgagggc gaggccggct ggccgtgggt
    16981 gcgcgagagc atcggccgcg ccgcatggcg gcgcgccagc ccgcgcagcg cgacgatgag
    17041 ctggtcgagg cgcggcagca ggacacgatc gcgcgagtgc gccagcatca gcgcataggc
    17101 gagattgttg atgtcctccg aggtgcaggc gaagtggatg aactccagcg agcgctgcac
    17161 ctcggcgttc ttcgccagcc ggcccttcag ccagtactcg atcgccttga cgtcgtgatt
    17221 ggtctcggcc tcgatgttct tgatgtgcgt ggcatctttt tccccgaagc ccgcgaccag
    17281 ccggtcgagc gcgaggcgcg tggcgcgcga gaagggtttc agctcgcgca ggcggcgctc
    17341 ggcggcgagc gccttcagcc attccagctc gacgagaacc cggtagcgga tgagcgccgc
    17401 ctcgctgaag tagggccgca gcgcctcggt ggacctttcg tagcgcccgt cgagcggcga
    17461 cagcgcggcg agcggggaag gcatgccgcc tattatagag gggcatgctc gaagccctca
    17521 tccgggaggc ccggcgctgc cgcgtctgcg cgccgcactt gccgctcggc ccgcgcccgg
    17581 tgttcatggt cggggcgggg gcgcggctgc tcgtcgtcgg ccaggcgccc gggcgacggg
    17641 tgcacgagac cggcatcccc tggaatgacc cgtccggcga gcagttgcgc gcgtggctgc
    17701 gcatggaccg cgcgcgcttc tacgacccca cgcgcgtcgc catcctgccc accggcctgt
    17761 gctaccccgg cagcggcgcc cgcggcgacc tgccgccgcg ccctgagtgc gcgccgctgt
    17821 ggcagccgcg ctttcgcgcc gcgctgcccg gcgtgcacct cacgctgctc atcggcctgc
    17881 atgcgcagcg ctattacctc ggcgcgcggc gcaagcgcac gcttgcacag accgtgcgcg
    17941 cgtatgcgga gtacctgccg gaattctttc cgctaccgca tccgagcccg cgcaaccgca
    18001 tctggatgcg caacaacccc tggttcgcaa gacaagtcgt cccggcgctc cgccggcgcc
    18061 tgcggacgct ataattcgcg cccgccatga agctgctctc tgcacccgcc agtccctttg
    18121 cacgcaaggt gcgcatcgtg ctcgccgaga agcgaatcga ctgcgacctc gagcgcgtgg
    18181 acgtgcagcc gcaggacaac ccggtcaacg cccacaaccc gctcggcaag attccgacgc
    18241 tgatcctgga cgacggcacc gcgatctacg attcgcgcgt catcatcgag ttcctcgaca
    18301 acatgacggc caacagccgc ctcatcccgc aggacaaccg cgagcgcatc gcggtgcgcc
    18361 gctgggaagc gctcgccgac ggggtgctcg atgcgggcct tcttatccgc tacgagtcga
    18421 ttcggccgcc gagcgagcaa agcgcgtcgt ggaccgaaaa gcaggcggcg cgcatgcgcc
    18481 gcggcctggc acagctgcag gccgacctcg gtggccggtc gtggtgccag ggcgagcgtt
    18541 atacgctcgc cgacatcgcg ctcggttgct gcgtggggtg gatcggcttc aggaagccgg
    18601 ccggcatcga ctggcgcgcg gactacaagg atctggcgaa gcactacgac aagctgatgg
    18661 agcgaccggc gttcgccgac accgttcctg ccgcctaggc gatcaccgcc ccgccgaggc
    18721 acacctcacc gtcgtagagc actaccgact ggcccggcgt gatcgcgcgc tgcggccgag
    18781 ggaagctcac cgcgacttca ctatccgcga tgcgggacac cgtgcattgg gcgtcggcct
    18841 ggcggtagcg ggttttcgcc ccatgcatcg acccgggcgc cggcgccggc cccacccagc
    18901 tcgagtccag cccgtccagc gacgagcgca tgagcagcgg atggtcgtgc ccctggacca
    18961 cgacgagctc gttggcgtgc atgcgttttt cggcgacata ccacggctcg ccggcgccgc
    19021 ccaggccgat gccctttctc tggccgatgg tatagaacgc gagcccgatg tgctcgccca
    19081 cgcgccggcc ttccggcgtc accatcgggc ccggcgttct cggcaggtag cgattgagga
    19141 actcacgaaa cggccgctcg ccgatgaagc agatgccggt ggagtccttc ttcgcgtggt
    19201 tcggcagtcc ggcctgcgcg gcgaggcgcc gcacctcggt cttcgtcaga tcgccgaccg
    19261 ggaacatgac gcgcgcgagc tgcgcctgcg tcaggcggtg caggaaatag ctctggtcct
    19321 tggcagcatc ggcgccgcgc agcagctcca cgccgttggc gcccgtgcgc agccgcgcgt
    19381 aatgcccggt ggcgattttg tgcgccccga gctgcagggc gtggtcgagg aacgcgcgga
    19441 atttgatctc ggcgttgcac agcacatccg ggttgggcgt ccgcccggcc gagtactccg
    19501 ccagaaagct cgcgaagacg cgctcgcggt actcggcagc gaagttcacc gcctcgagcg
    19561 gcacgccgat gacgtcggcg gccgcggcgg cgtcgatcag gtcctcgcgc gtcgagcagt
    19621 aggcatcatc gtcgtcgtcc tcccagttct tcatgaacag gccgacgacg tcgtacccgg
    19681 cgcgcttcag cagcagcgcc gcgaccgacg agtcgacccc gcccgacatg ccgacgacga
    19741 ccttttcacg catacggctt ggacagcacc tcgagcggaa agctccggcc cgcgagatag
    19801 tcgtccacgc actgctgcac gagcggtgag cggtgctcgg tgcgccgcgc ggcgagctcg
    19861 gcaggcgtca gccaggcgag cgcgatgatc tccttgtcga gctgcctgcc gtcctgcacg
    19921 ccgtccaccc ggccgttgaa tgcgaagcgc aggaaggtca cgtcctcctt gtcgtagcgc
    19981 caccggtaga tgccgaccag gccggtgggc gtgaaccgat gcgcggtctc ctcgagcacc
    20041 tcgcgcgcca cggcctgcgc gagcgactca cccgggtcga ggtgcccggc gggctggttc
    20101 agcacgcgcc gcccgttcgc ctcctcctcg acgaagagaa agcgcccgtc gcgctcgatc
    20161 agcgccgcga ccgtcacgct cgggcgccag cggctcatgc cgccttgcgc gtgttcttct
    20221 tgatgaactc cgccatgcgc gccacaccct gctcgatcgc ctgccgggag gcggcgtacg
    20281 aaaaacgcac gtgctgaccc tcgcccggga cgcgcgcgcc gaaatggatg tcggcgagca
    20341 ccgcgacgcc cgcctcgttc agcaagcgct tgcggaactc ctcggagtcc gtggcgccga
    20401 tgcgcttgca ggcctcggtg acattgggcc aggcgtagaa cgccccgccc gggttgcggc
    20461 aggcgacgcc cggcacctgg ttgagcagcc cgacgatcag gtcgcgccgc tcgaggaagc
    20521 tggcgcgcat cttctcggcc gcatcctgcg ggcccgtaat cgcctccacc gccgcccact
    20581 gagagatctg cgaggcgcag gactccgtat tggtgatcca gcgcgtgaac actggcgcga
    20641 gcgaaggatt ggaggtaaag ccgatgcgcc agccggtcat ggcccaggtc ttggaggcgc
    20701 cgtcggaaat gatcgtgcgc tcgtacatct ccggcagctg cgcgatggag aagaactcgc
    20761 ccgcgtagca caggcgcgag tagatctcgt cggcgtacac ccacacctgc ggatgcttgc
    20821 gcaggatcgc ggcgatggcc tccagatcct tcttcgtcag cgtcccgccg gtggggttgt
    20881 gcgggtaatt caggatcagg agcctggtct tcggggtgat gagccgctcc agctcctgcg
    20941 ggtcgaaggc gaattcacgc gcctcgtgca ggtggatcgg cacaggcttc gcgccgttgg
    21001 cgagaatctg cgactcatag atcgggaatc ccgggttggg atagatcacc tcgtcgcccg
    21061 cgttgtagtc agtggtggac aggatggcgt aggcgatgaa cggcttggcg cccgcgccca
    21121 ccacgacgtc ctccgcacgg atcggcaggc caccgcgcat ccgtgccatg taggtcgcgg
    21181 ctgccgcgcg cagctcgtcg atcccggccg agggcgtata gccgtgcttg ccgccgcgga
    21241 tggcgcgaat cgcggcctcc tgcacgtgct caggcgccgg gaagtccggc tggccgatgc
    21301 aaaagctgat gatgtccttg ccctggcggg cgagcgcgtt cacctccgcg aggacaacga
    21361 aggcgttctc ggtgccgagg tggcgggcgc ggcggctgat atcgggcata cggtccccct
    21421 gaagcgcgca ttttacagtg ctacgatccg cgaccatgct ggtcaactgc gtggtctacc
    21481 aggacggcaa gaagctcgcc gacatccaga agcgcgagat cagggcctac ctgcagcggc
    21541 ccgagtgttt cgtgtgggta gcgctgcgcg atcccgacgc cgccgagctt gccgagatgc
    21601 aggacgagtt cgacctgcac gaactcgcgg tcgaggacgc gcgtcacggc aaccagcggc
    21661 cgaagctcga ggagtacggt gactccctgt tcgcggtgct gcacaccgtg gaagtggccg
    21721 gcgacgagct gaaggtaggt gaggttgacg tcttcgtcgg ccgcaactac gtgctctcgg
    21781 cgcgacagaa gactgagcgc ggcttccagg acgtgcgcgc gcgctgcgag cgcgagcccg
    21841 agctgctgcg ccacggttcg ggctacgttc tctatgcatt gatggacgcc gtggtggacc
    21901 gctatttccc ggtgctcgcc gcagtggaat cgcagctgga gaagctcgag gcgcagatct
    21961 tcaccggcaa gtcgccgcgc gagaacatcg agtcgctcta ctacgttaag cagaaactga
    22021 tgacgctgaa gcacgccgcg gggcccctgc ccgagacgtc gagccgcctg tttggcggcc
    22081 gcgtgccgcc ggcctgcgcc ggcctggggg aatacttccg cgacgtctac gaccacgtgg
    22141 tgcggctcaa ccagtcgatc gacaccctcc gcgacaccgt gaccaccgca attcaggtga
    22201 acctggcgat gatcacgatc ggcgacagcg aagtcaccaa gcgccttgcc gcgtacgccg
    22261 cgctggtggc cgtgccgacg atgatcgccg gcatctacgg catgaacttc gcgaacatgc
    22321 ccgagctggg ctggagcttc ggctatccgc tgtccattgc catcatggcg gcaatcgacg
    22381 ggtacctgtt ctaccgcttc cgcaaggccg cctggctcta gctctagctc tagcgctcgc
    22441 gctagcgcat cccggggagc gcgcccttga ggttgcgcat catgcgcgcc atgttgccgc
    22501 ccttcatcat cttcatcatc ttctgcatct gctcgaattg gttcagcagg cgattcactt
    22561 cctgcaccgg gacgcccgcg ccggcggcga tgcgccgctt gcgcgaggcc ttgatcagct
    22621 ccggatgcgc gcgctcctgc ggcgtcatcg cgttgatcac gcccgcggtg cgccgcagct
    22681 ggcggtcgtc ctgcagctgc gccgggttga tctgccccgc gagctgggcg ggcagcttgt
    22741 ccagcatcga ggccattccg cccatcttct gcatctgcag cagctgctgc ttgaagtcct
    22801 cgaggtcgaa gcccttgccc ttcttcagct tctcggcgac gcgctgcgcc tgctcgaggt
    22861 cgacctgctt gtgcgcctcc tcgaccagcg acaggatgtc gcccatgccg agcacgcgcg
    22921 aggccatgcg ctcgggatgg aacggctcca gcgcgtcgag tttttcgccc acgcccgcga
    22981 acttgatcgg cttgccggtg acctggcgca ccgagagcgc cgcgccgccg cgtgcgtcgc
    23041 cgtcgagctt ggtgagcacg acgccggtga gcggcagccg ctcgccgaag gcgcgcgcca
    23101 cgttgactgc gtcctggccc tgcatcgcgt cgaccacgaa cagggtctcg actggcttga
    23161 cctggccgtg cagcgccgcg atttcctgca tcatcgcctc atcgatgccg aggcgccccg
    23221 ccgtatcgat gatcagcaca tcgtagtagc gcgtctttgc gtgctcgagc gcgcggcgcg
    23281 cgatgtcctg cggcgcttcg ccgacggcag acgggaacac gtccgcgccg gcctgcgccg
    23341 cgacagcggc gagctgctcg atcgccgccg gacggtacac gtcgcaggag acgagcagca
    23401 ccttcttctt ttgcccgagg agcagccgcg cgagcttgcc cgcggtggta gtcttgcccg
    23461 agccctgcag gcccgccatc aggatcaccg ccggaggctg cgccgcaaga tcgaggccgg
    23521 catgcgcgcc gcccatcagc gccgcgagct cgcggtgcac cacgccgatc agcgcctgcc
    23581 cgggcgtcag gctgccgacg acctcctcgc cgagcgcctt ctgcttcacc gcggcgacga
    23641 aatcgcgcac caccggcagc gcgacgtccg cctccagcag cgccacacgc acttcgcgca
    23701 gcgcgtcctg gacgttggct tcggtgaggc gcgcctcacc gcggatcgtc ttcacgatcc
    23761 gcgccaggcg cgcggtcagg ttctcgagca cgggtgataa actgaagaag tgcccgacat
    23821 tgtaattcac gcggcgactg cggccgccta tgcggggctg gcgtggcact tctggcgcac
    23881 gcgctggcgc gcgcagcccg ctggcacggt gccgctcgcc ggcacccggc cgtgggagcg
    23941 agtggcgctg ctcgcgccgc tcgccctgca cgcctacctg ctgtacgcgg agctgttcgc
    24001 gccgtccgag ctgcgcttcg gcttcgccca ggcgctttcc acgatgctgt ggctcgcggt
    24061 gctgatctac tggttcgaaa gcctgttctt cagcctggaa ggcatgcagc ccccggtgct
    24121 ggcgctggcg gcgctcgccg cgcccctgcc ggcgctgttc cccgggcgcg ccagcgttct
    24181 ggccgcctcg ctcgagttca aggtgcacct ggcgatggcg atgctcgcct acagcctgtt
    24241 caccattgcg atcctgcacg cgctgctgat ggcgctgctc gagcgccgtc tgcatggcag
    24301 cgcggccgca cgcgccgcgc tgcccgccga gggcgccggc ctgctctcgg gtcccctggg
    24361 cgcgctgccg ccgctgctca cgctggagcg gctgctgttc cgcatgatcg ctgccgcctt
    24421 cgtgctgctc acgctgaccc tggcgagcgg catcgtgttc tcggaatcga tgttcggccg
    24481 ggcgctgcgc ttcgaccaca agacgctgtt cgccgtgctg tcgtgggcga ccttcgcgct
    24541 gctgctcgcc ggccgctacc tgtacggctg gcgcggccgc atcgcgctgc gctggacatt
    24601 gagcggtttc ctgatgctcc tgctcgccta catcggcagc cgcttcgtac tggaagtcgt
    24661 gctcggacgc ggctgacatg gaagacctgc cgatcggctg gtccttcggc gcgctcgccg
    24721 tgctgctcgt gctctcgggg ttcttttcca tcgccgagac cagcatgatg gcgctgaacc
    24781 gctatcgcct gcgccacctc gtggcgcagg gccacggcgg cgccaagcgc gctgccgagc
    24841 tgctcaagcg caccgacacg ctgctcggcg ccatcctcat cggcaacaac ctggtgaacg
    24901 ccgccgcggc cacgctcgcc ggcctgatcg cggtgcgcct gatggggcag gacaagctcg
    24961 cttacgcggt gagcacggtg gcgatctcgt tcctgctcat cgtgttcgcc gagatcacgc
    25021 ccaaggtgat cggcgccacc tatcccgagc gcatcgcgct gccgttcgcc tacgtgctgg
    25081 ggccgctgcg cagcctgctg cggccggggg tgtggttcgt gaacctgttc gtgcggccgc
    25141 tgctgctgct cgcgggcgtg cggcccggcg agagcacgga ggtgcaaaag ctcacgccgg
    25201 cggagattcg cacgctggtg ctcgagtctt cgagcttcat gcccaaaaag cacgtctcca
    25261 tcctgctcaa cctgttcgac ctcggctcga ttaccgtgca ggacatcatg ctgccgcgcg
    25321 cgcgcatcga atcggtgaag ctggacgacg acacggcggt catcgcgcgc cagctcggca
    25381 ccagctacca catgcgcctg cccgtgttcc gcgactcgcg cggcgacgtg ctgggcgtgc
    25441 tgcacctgcg ccgggtgctg gcgccgctca tggcgggcac gctcgacaag gcgcagatcg
    25501 aggcgctgct cgcgccgccc tatttcgtcc cggcgaccac gcccgtgttc gcgcaaatgc
    25561 agtacttcca ggaaaacgag gagcgcatcg ccctggtggt ggacgagtac ggcgagctga
    25621 tgggtctggt gacgctcgag gacatcatcg aggagatcat cggcaagttc accacctcgt
    25681 tgccggccgc ggcgcatgcg ctcgcctggg ccgcggacgg cagcgcgacg gtggacgggg
    25741 ctacgccggt gcgcgaggtg aaccgcgccc tcggcctcga tctgcccacc gaggggccga
    25801 agaccctgaa cggcctcatc ctggagcacc tgcgcgacat ccccgaggcc gacgtgtcga
    25861 tcaaggttgg cgaggtgccg ctcgagatcg tccatgcgca gggccggagc gttaaaacgg
    25921 tgcgcatctt tcgcccgccg gcgccgccgc ccggcctcgc cgaggcaccc tgaaccgggc
    25981 gttgcgggcg ccccaaaacc aagacagctc agcggcttag gcgagaaacc tgaggcttta
    26041 caaaacactg gccgcaaggc atcatcgccg gtcagtcctg cagcgacggg catagggatc
    26101 tagggagcgc tcgatggcaa cagcagcggc gaaggccgcg gggcccaagg agttcaactt
    26161 cctttgggaa ggccgcgata agggcggcaa ggtcatccgc ggcgagctgc gctcggtgag
    26221 cgaggcggcg gtcaacgcca ccctgcgccg ccagggcatc atcgtcaaca aggtcaagaa
    26281 gcagaagtac ggctccggcg ggaaggtcac cgacaaggac atcacgctgt tcacgcgcca
    26341 gctggcgacc atgatgaagg ccggcgtgcc gctgctgcag tctttcgaca tcgtgagcaa
    26401 gggcgcatcc aacccggcag tggcgcggct gctgctcgag gtgaagaccg aggtcgagac
    26461 cggcaattcc ctcgccgcgg cgttccgcaa gtacccgctg catttcgacg ccctgttctg
    26521 caacctggtg gctgccggcg agcaggcggg gattctggag acgctgctcg accgccttgc
    26581 cacctacaag gaaaagatcc tcgccatcaa gtcgaagatc aagtcggcgc tgttctatcc
    26641 catcgccatc atcgtggtgg ctttcgtcat cacggcagtg atcatgatct tcgtgatccc
    26701 ggcgttcaag gaggtgttca ccaacttcgg cgccgacctg ccggcgccga cactgatggt
    26761 gatggcgatc tccgactggt tcgtcgccaa ctggtacatc atctttcccg cggtgatcgg
    26821 cggcgtgatc ggtttcctgg aggcctggaa acgctcggtg cctgtgcaga tcttcatgga
    26881 tcgcctgatg ctgaagctgc cggtgttcgg cgacctggtg ctgaagtcga ccatcgcgcg
    26941 ctggacgcgc acgctcgcca ccatgttcgc ggcgggggtg ccgttggtgg aagccctgga
    27001 ctccgtcggc ggcgcttcgg gcaaccacgt ctacctcacc gccaccaaga agatccagac
    27061 ggaggtctcg accggcacct cgctcaccat ggcgatgcag aactcggggg tgttcccgaa
    27121 catggtgctg cagatgtgct cgatcggcga ggaaacgggc gccctcgacg cgatgctcgg
    27181 caaggtggcg gacttctacg aggccgaggt cgatgacgcg gtcgaagcgc tgtcctcgct
    27241 catggagccc atgatcatgg tggtgctcgg cacgctgatc ggcggcatgg tgatcgccat
    27301 gtacctgccg atcttcaaga tgggccaagc cgtctagatg gcggcgttcg gctggctggc
    27361 ccagcctgcg gtcctgccct gggtagcgct ggccctgggt ctgtgcatcg gctcgttcct
    27421 caatgtcgtc atccatcggc tgccgaaaat gatggagcgc gagtggcgcg agcagtgcgc
    27481 cgagctcgcg ggcacgccgg cgcccgcggc cgcgtcggcg cacgggcgct acaacctgct
    27541 ggtgccgcgc tcggcctgtc cggcctgcgg tcaccgcatc accgctctgc agaacatccc
    27601 gatcgtcagc tggctcgcgc tgcgcggcaa gtgcgccgcc tgcggcacgc gcatcgcggc
    27661 gcgctatccg ttcgtggagc tggtcgcagg cctgatcggc gcctacgccg cgtggcgcta
    27721 cggcgcgacg ctcgctgcgc tcggcgcggc ggcgttcggc tgggcgctgc tcgcgctggc
    27781 ggtgatcgat ctcgacacgc aactgctgcc cgatgacatt acgctgccgc tgctctgggc
    27841 cggcctgctc gtcaacttcg gcgccgtgtt cgcgccgctg cgcacggcgg tgatcggcgc
    27901 ggtcgccggc tacgtctcgc tgtggctggt gtactgggca ttcaagctcg ccaccggcaa
    27961 ggaaggcatg gggcacggcg acttcaagct gctcgcggcg atcggcgcct ggctcggctg
    28021 gcaaaagctg ccgctggtga tcctgctgtc gtcggtggtg ggcgccgccg tgggcatcgc
    28081 gctcatcgtg ctcgcacgcc acggccgtga gaagccgatc ccgttcggtc cctacctcgc
    28141 cgccgccggg ctgatcgcgc tgttctgggg cgacgtcatc acgcgccgct ggctgccgat
    28201 cctcggttga tggcgttcat cgtgggcctc acgggcggca tcggcagcgg caagagcgcc
    28261 gccgcggacg agttcgcgag gctcggcgcc gccgtcgtcg acaccgacgc catcgcgcac
    28321 gagctgaccc gcgccggcgg tgccgcgatc gcgggcgtgc gccgcgtgct cggcgacgag
    28381 ttcgtcgacg cttcgggcgc gatggaccgc gccaaggtgc gctcgctcgt gttctcggac
    28441 cgtgccgcgc gggcgcgcct cgaaggcctg ctgcatccga tgatccgcgc cgaatccgag
    28501 cgccgcatcg gcgcggccac cgccccttac gtcgtgcacg tggtgccgct gctcgtcgaa
    28561 tcgccggact accgccagcg cgtgcagcgc gtgctggtcg tcgactgttc gccgcaggtg
    28621 cagaaggcgc gcgtcgggca gcgcagcggg ctggccatcg acgaggtcga gcgcatcatc
    28681 gacgcgcaga tcccgcggga aacgcgcctc gccgcggccg acgacgtgat cgacaactca
    28741 ggttcgcttg acgcgctgca caagcaggta cgggcgctgc accggcgcta cctggacatg
    28801 gcgcgcaccc cggcatgaat ttgtcatcgc ggcgtaaatc actcagaatc cgccaaattt
    28861 tcgcagggcg gccgtgatca catacgagta cccgctcaac gagcgcatcc ggaccttgct
    28921 gcggctggag gatctattcg agcgctcgcg ccatttcatc gcgcgcaccg acccgctcga
    28981 tcaccatgtc gcgcttctga ccttgttcga gatcctggag gtctcgagcc gcgccgacct
    29041 gaagtccgac ctgctgcagg agctcgagcg ccagaagcag gtgctgctct cgttccgcaa
    29101 caatccggac atctcggagg aggcgctgtc cagcgtgctg cgcgacatcg agcaagcggc
    29161 ggcggcactc ttttcgatga ccggcaagat cggccagtat ctccgcgaga acgactggct
    29221 gatgtcgatc aagcagcgca ccggcatccc gggcggcgct tgcgagttcg acctgccgtc
    29281 ctaccacttc tggctgcacc gcccggccga agagcgcatc ggccagctcg cggcgtggac
    29341 cgggccgctg ttcccgctgc gcgacggctc cgcgatcatc ctgcggatcc tgcgcgagtc
    29401 cggcaagccc cagcgcctgt cggcgccgct cggcacgttt cagcagatgc tgggcggcaa
    29461 gacggcgcag atgctgcgcg tgcgactcga cccggccgca aactgcgtgc ccgaaatcag
    29521 cgccaacaag tacgtgctca acatccgctt cctgacgcag gcgagcgagg atccgaagcc
    29581 gcgtgccgcc gactgggacg tcggcttcga gctcacgttt tgcaacctgt aagaacggtc
    29641 ccctgcccgc gttgcggcat gccggcgccc ttcgccgcgg caaacccgtg gcgcccgttc
    29701 tgcagcgagc gctgccggct gatcgacctc ggcaactggg ccgccgagag ctaccgcgtg
    29761 ccggagtccg gaccgcggcc gcctgacgag accgactaag cccaggcgcc gccgatcatc
    29821 gccacgccgt gcgcgcccgc gcgccaggcg gcctcgaggt cgcggcggcg caggccgccg
    29881 atcgcgtaga ccggaaggct cgcgccgcgc gcgatgcgcg cgaagccgtc ccagccgagc
    29941 aaccgggcgc ccgggtgcga ccgcgtcggc tcgacggccc cgaggaccgc gaaatcaagc
    30001 tccagcgcag ccgcgcgcgc cagctcctcc ggcgtatggc aggaggcggc ggcgaggcct
    30061 tcgcgcggcc gcgactccag ccgcatgagc tgcgccgcgg tgtagtgcac gccgtcggct
    30121 tccggcgcgg gcgccccgct caccagcacc cgagcgccgt ggcgatgcgc aaggcgcacg
    30181 acgtcgcgca cgaaggcctc gcggccggcg agctcccggt cccgaacctg gatcagctta
    30241 agcccctgct tgagccgcac gccgatacgc gacagcatga cccgcgtacc ttggctttgg
    30301 gcatccgtaa tcgcgtactc gtgcggcagc gcgagcgccg cgagcaccgg cgcatttgcc
    30361 ggcagcatcg gtgccgcgag cggggtaccg aagcgctgcc aggcaattgc ctgctgctcc
    30421 ttcggttgtg gatcgccctt ccaggcggtg acgcggaaaa agttcagccg caccgatgcg
    30481 tgcggatagg tgtactcccg cgtcagccag ggaaagctgg tgcaagcctc gatgccgagc
    30541 tcctcgcgca gttcgcgcgc cagcgcccgc tcggcggcct cgcccggctc gaccttgcct
    30601 ccggggaact cccagtagcc cgcgtagact ttgccggcgg gacgctgcgc cagcaagaac
    30661 gctccgtcgg cgcgctggat gacggccgcc gccacctcca gcgccacgct acttcgcccc
    30721 ggcgtgcttg cccgcccagt cgcgcgcgaa ctggtaagcg atgcgtccgc tcctcgagcc
    30781 gcgctgcagc gaccattgca gcgcctcctc gcgggcggcg gccgccgcct tgggtccgca
    30841 gcccagggtc ttcagccagt gcgccacgat ctccaggtac tcgtcctggt cgaacggata
    30901 gaaagagacc cacacgccga agcgctccga gagcgagatc ttctcttcca ccgtctcgcc
    30961 gggatgaatc tcgtcgccga cgtatttggt ctccaggttc tcggccatgt actcgggcat
    31021 caggtggcgc cggttggacg tggcgtagat cagcacgttc tcggaggtgg tcgaaatcga
    31081 gccgtcgagt gcgaccttga gcgcgatgta gccgtcctcg gcgccgtgga aggtcaggtc
    31141 gtcgcagaac acgaggaaac gctcgtcgcg ccccgctacc tggtcggtga tcgccgccag
    31201 gtcgacgagg tcggtcttct ccacctcgat caggcgcaac ccctgcttg
//
LOCUS       DBNHKE010000199        15767 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1146953, whole genome shotgun sequence.
ACCESSION   DBNHKE010000199 DBNHKE010000000
VERSION     DBNHKE010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1146953"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..169
                     /locus_tag="AC16O9_13625"
     CDS             14..169
                     /locus_tag="AC16O9_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517689.1"
                     /translation="MFSLFLLYDLQRIIRGGETNYVMATTGVYMSLFNIFANLLYLLM
                     ALSGQRE"
     gene            408..1178
                     /locus_tag="AC16O9_13630"
     CDS             408..1178
                     /locus_tag="AC16O9_13630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6517690.1"
                     /translation="MQGSPRTIARRWARWLIALAAIGFWVWLFLVQLTMPPMEHSLGF
                     DAWYGNWRDAFIVSAVFLAFVLGFAWPRGGMQWRNAGMYSAFLISLFVEMFGIPLTIY
                     VIAPLLDLRALDFGMNESHLWAYVLDRAGLVPLAAGARLVMIVSMALIALGAALVAVG
                     WAQVYGARDELRTSGLYRLMRHPQYLGLILVVVALVVQWPTILTLAMAPLLVLMYVRQ
                     ARREDRDLEARFASAFAAYAARVPAFIPRMPLSPRERA"
     gene            1215..1871
                     /locus_tag="AC16O9_13635"
     CDS             1215..1871
                     /locus_tag="AC16O9_13635"
                     /inference="COORDINATES: protein motif:HMM:NF023287.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HIR6517691.1"
                     /translation="MDCLEFRRTVLSDPRRPGAVAEAHAAGCAACREFLTRSLEDEDE
                     LDRALHVAVPEGLQDRVLRRARATGARVRWLAHAASVVVAVALAVVWPRDDALALTGI
                     DFVMFEEAQSIADAAPTDWNVPAAAARDMGVSLPRALGEMRCVYPLAAGAAHHLLVRT
                     PFGKLTLLLIPDHASASRAAGAAYGFNAAVLPAGRGTVVIIGDSLRSVQCAETLLKSA
                     "
     gene            1933..2598
                     /locus_tag="AC16O9_13640"
     CDS             1933..2598
                     /locus_tag="AC16O9_13640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6517692.1"
                     /translation="MPDRALALSRYRRLARGYDAATHGIERKRRRAIDLLRLRPGETV
                     LDAACGTGSALAPLARAVGPGGRVIGVEQSAEMIAIARQRCAALGLGNVVLLEAPVEN
                     ARIAGPVDAVLFSFAHDVLQSPAALANVFAAARPGARVASVGAKLYPRWLSFLNFWVR
                     WRIRDYVSTLEGLERPWRLLAEFVPDFTVARVTYLGSGYIGGGSYPGIAAGRASIAAP
                     LCA"
     gene            2613..2975
                     /locus_tag="AC16O9_13645"
     CDS             2613..2975
                     /locus_tag="AC16O9_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517693.1"
                     /translation="MWIAVLTLGALGFAQASVSLAACSMERGTISDVLAAKTSAPCDC
                     GTAMTEFGPLFANRCVAHCTSGLQLSGAAAGIAHVAAPAPTLVRPRAQQRVTWRRRAQ
                     MPPVASVPKRIQLHSFLV"
     gene            3067..4311
                     /locus_tag="AC16O9_13650"
     CDS             3067..4311
                     /locus_tag="AC16O9_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6517694.1"
                     /translation="MRSSLFITACCALAAAPALAQSLSLQEALRLGETQSPRLSAQRF
                     AASAAAEQVGRAGELPDPKLRLGLENLPVTGEDRLRYDRDFMTMRSVGLMQEFPNADK
                     RAARNARAVRLHELEGASVHAQVSALRREIAVAWLDAYYAERTREALERRGRQFRLQA
                     DAIAAAIARGRQGAADGFMLQQALETNRLRVIEQERQLERARLALGIWIGEVARREFA
                     PAPDTARLAEAPEQMLARLERHPALRTSEQREALARAEVDLARSAKKADWALELGYAQ
                     RRPSFDNMVSVMATFELPWWAGRRQDRDVASRLAELEQARAMRADERRVREAELRGWI
                     ADYDAATRRIERLERTLLPLARERAAAAAAAFRGARGELAPVLESEGAVTEMEVELVQ
                     AQAERGRAWANLTYLFAEEGQP"
     gene            4308..5813
                     /locus_tag="AC16O9_13655"
     CDS             4308..5813
                     /locus_tag="AC16O9_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6517695.1"
                     /translation="MNRTTLGLAIATALAAGAYGGYQIAQYRAPETHAGAASVPHPEA
                     APEKRVLYWFDPMYPQHKFDQPGKSPFMDMQLVPKYAEPERDAGTVVINPRVVQNLGV
                     RTAEAALGALERRFDAVGTVAWNERAVVQMQARAAGFVERLHARAPLDAVKKGAPLVE
                     LLIPEWAGAQEEYLVLLASDASEARALARAARQRLVLLGMSEADIAAITRERRVRERF
                     TLASPISGVIAELGVREGAAVAPGQTLYRLVDLSTVWVNAEIPEAQAGLVVPGSRVEA
                     RVPAYPDHLFGGKVGAILPEVSAATRTVRTRIELDNHEGLLKPGMFATLRFSSGNGRE
                     QVLVPSEALIRTGTRSVVILALGEGHFRAADVEVGQEGGGRSEILKGLKPGERVVLSS
                     QFLIDSEASLSAATARLQEGDEPAPVAASDRHTGRGKVTGLEIAGARVELDHGPIPAL
                     QWPAMRMEFKLADPKQLDGLAQGDEVQFELRGEPDKDGDYVIERITRRPGR"
     gene            5810..8926
                     /locus_tag="AC16O9_13660"
     CDS             5810..8926
                     /locus_tag="AC16O9_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6517696.1"
                     /translation="MIAALIRWSVHNRYLVILATILVAAWGVVSVLRTPLDAIPDLSD
                     TQVIIRTPFPGQAPRIVEDQVTYPLATTMMSVPGAKTVRGYSMFGDSYVYVLFEDGTD
                     LYWARSRVLEYLNQVQGRLPASAKPALGPDATGVGWIYQYALVDRSGRHDLGQLRALQ
                     DWFLKYELKSVPDVAEVASVGGMVRQYQIVLFPERLRAYGISHNRVIDAVQRANRETG
                     GSILELGEAEYMVRSGGYLKSLEDFAAVPLGVSDGGTPILLRDVARIQMGPEMRRGVS
                     ELDGEGEAVGGIIIMRHGRNALETIAAVKRKLASLKPALPPGVEIVETYDRSGLIVRA
                     VRHLAEKLAEEFVVVALVCLAFLLHLRSSLVAIVTLPLGVLAAFIVMRFQGVNANIMS
                     LGGIAIAIGAMVDAAIVMIENAHKRLEAWRHANPEGELSGAARWRLIEEAAAEVGPAL
                     FFSLLIITLSFIPVFALEAQEGRLFSPLAFTKTYAMAASAILSVTLVPVLMGYLIRGR
                     IPDERRNPLNRLLITAYRPLLAAVLRAPRTTLVVAALVAASTLWPIARTGTEFIPPLD
                     EGDLLYMPTALPGLSPGKAAQLLQQTDKLIATVPEVERVFGKIGRADTATDPAPLEMV
                     ETIVRLKPRAEWRPGMTPEKLIEELDRLVQFPGLANLWIPPIRNRIDMLATGIKSPLG
                     IKVSGDDLGAIEGAAASIERAVKTVPGVTSALAERVRGGRYIEIGIDRAAAARYGLNI
                     ADVQSVVSAAIGGENIGETIEGRQRFPINVRYAREVRDSVDKIRNLPIVTERGAQIPL
                     AAVASVAIADGPPMLRSENARLNGWIYVDIRGRDLGSVVADAQRAVAREVKLPPGVSV
                     AWSGQFEFLERARARLTLVVPFVLLIILVLLYMTFQRMDEALLIMVSLPFALVGGFWT
                     LYALGHNLSVASAVGMIALAGVAAEFGVVMLLYLKQAIERREAAPAHGGLLEALDEGA
                     VQRVRPKAMTVAVILAGLAPIMWSEGTGAEVMQRIAAPMIGGMITAPLLSMFVIPAAY
                     YLMRRGKA"
     gene            complement(9035..11305)
                     /gene="selD"
                     /locus_tag="AC16O9_13665"
     CDS             complement(9035..11305)
                     /gene="selD"
                     /locus_tag="AC16O9_13665"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR6517697.1"
                     /translation="MLTPDQPVLRDIVLVGGGHSHVGVLRRFGMKPMPGVRLTLICRD
                     THTPYSGMLPGYIAGHYAYDEVHIDLARLAQFAGARFFRDEVVALDRGARKAICRSRP
                     PVTYDRLSINIGSTPRVQDVPGAAEHAVPVKPIHDFNARWLALLERVRAHPGKARIAV
                     VGAGAGGVELALAMQHRLRHELRARGRDPGDIEFHLLDAGPRILPTHNAAVRRTFERV
                     LRARGVVMHTDAGVVQVSPGRLRTSRGDALDVDEIVWVTRACGARWLRDTGLELDADG
                     FIRVAETLQTLTDPDIFAAGDIASMVDHPLEKAGVFAVRQGPPLAENLRRAVTGRQLA
                     RYRPQRRWLALISTGDKSAVASRGLLYLRGDWGWRWKDWIDRRFMRRFGELPEMSAPP
                     AGAPPAVRLDAEEAAQAISAIAMRCGGCGAKVGATVLSRALGSLQPVPRAEVLIGLHA
                     PDDAAVVRVPPGKALVHTVDFFRAFLDDPYLFGKVAANHALGDIFAMGADPQSATAIA
                     TVPAGLESKVEDVLRQMMSGAIEVLNEANCALVGGHSGEGRELALGFAVNGLIDEARV
                     LRKGGMRAGDVLILTKPIGTGTLLAAHAKLAARGRWIDAALASMTTSNRLGAQCLLRH
                     GATACTDVTGFGLLGHLVEMTRPSAVDAEIELSALPVLEGAEEMAAAGILSSLQPANV
                     RLRRALSNQEQMVSHPRYPLLFDPQTAGGLLASVPAAQAQDCLKALRADGYPRAAVIG
                     RVLPQGEALAPITLRP"
     gene            complement(11508..11717)
                     /locus_tag="AC16O9_13670"
     CDS             complement(11508..11717)
                     /locus_tag="AC16O9_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HIR6517698.1"
                     /translation="MSAAVLESTLTCPVCGGRSSEVMPSDACLFFWSCPACATRIKPK
                     PGDCCVFCSYGSVACPPKQMGDSCA"
     gene            complement(11714..11944)
                     /locus_tag="AC16O9_13675"
     CDS             complement(11714..11944)
                     /locus_tag="AC16O9_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6517699.1"
                     /translation="MAAEPARVTLDVPSMNCSLCPIAVSGVLRKQPGVHEVRADLATK
                     TAQVVFDPEKTSPARLAQAVSDAGYPAAPRAP"
     gene            complement(11970..12320)
                     /locus_tag="AC16O9_13680"
     CDS             complement(11970..12320)
                     /locus_tag="AC16O9_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HIR6517700.1"
                     /translation="MQKPAAETASIAAGAASALGASTCCVLPLVLVSMGFGGAWIAQL
                     RALEQFQWVFIGLAAAAFGYAFYRLYLRPTTCGPELACTPASRRNQRIAFWATLVAAK
                     ALLLAPFYVPYLLG"
     gene            complement(12467..13087)
                     /locus_tag="AC16O9_13685"
     CDS             complement(12467..13087)
                     /locus_tag="AC16O9_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517701.1"
                     /translation="MAAPAWSGDSISVLERRELALERGTPAHTHRLSLVLAYFKGSWE
                     PARIHTALRKTTAILAQCGVAIAKAELALIGAPARYRDFHVPASRELARALALPRPTI
                     YFVAETRQRPAFEAEAIGRGNSASRPELRDTVWITRGARDLDVVLAHELAHVLMDSGE
                     HSAAPDNLMREDTAPGKVHLDDAQCERLRTTASANGLLRPVAPGSR"
     gene            complement(13136..14182)
                     /locus_tag="AC16O9_13690"
     CDS             complement(13136..14182)
                     /locus_tag="AC16O9_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6517702.1"
                     /translation="MSRGALYALGAAALFGASTPFAKLLLGAVSPWVLAAVLYLGSGI
                     GLAAWVALRSRLSPREPVAPIPSADWPWVAAAIAAGGVAGPILLMQGLARTDASGASL
                     LLNLEAVLTAAIAWTVFRENVDRRVFAGMMAIVAGGALLSTGAAPQAGSFAGVLLVTA
                     ACLAWAIDNNLTRRVSGGDAATLACLKGLAAGAVNLALAWVLGAPFPALSGWLAAGLL
                     GFLGYGVSLVLFIVALRGLGTARTGAYFSVAPFFGAVLAIAVLGERPGMAFWLAAGLM
                     ALGVWLHLSERHEHLHSHEAMAHTHEHVHDAHHRHTHDFPWDGREPHVHPHRHEPLVH
                     SHPHYPDLHHRHGH"
     gene            complement(14225..14539)
                     /locus_tag="AC16O9_13695"
     CDS             complement(14225..14539)
                     /locus_tag="AC16O9_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6517703.1"
                     /translation="MRIGELAKRADCPVETVRYYEKEGLLAAPGRPGADCAPVDALLD
                     EHIAHVAARIADLTALKQELDAIRAHCAGRRGARACGIVRSLSRPAARRARNNSHVRG
                     SS"
     gene            complement(14497..15147)
                     /locus_tag="AC16O9_13700"
     CDS             complement(14497..15147)
                     /locus_tag="AC16O9_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR6517704.1"
                     /translation="MSAACCPAQAHAADPAYRRVLAVALALNALMFLVELVSGIAASS
                     SSLLADAVDFAGDASNYALSLGALLLVPAWRPRVAYFKGLCMGAYGVLVLAITAWHAQ
                     RGTLPDATTMGVVALLALVVNVGVAASLYRYRTGDADMRSVWLCSRNDAIANVAVIAA
                     AAGVLGSGTGWPDWIVAALLASLALTSSYSVVRQARREILQGQCASESSPSAPTVP"
     gene            complement(15196..15495)
                     /locus_tag="AC16O9_13705"
     CDS             complement(15196..15495)
                     /locus_tag="AC16O9_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517705.1"
                     /translation="MRSARRGLPNDPLIAIIQPLIKFLGAPNAGIDASLHRFADSTIL
                     EGFWRNGRERGFWRLHLPLETVMPRETRVVKLPAGARKRRATGTRNARKRVRRAA"
BASE COUNT         2347 a         5445 c         5628 g         2347 t
ORIGIN      
        1 gctggtgatc gtggtgttct cgctgttcct gctgtatgac ctgcagcgca tcatccgcgg
       61 cggcgagacc aactacgtga tggcgaccac gggcgtgtac atgagcctgt tcaacatctt
      121 cgccaacctg ctctacctgc tgatggcgct ctccggccag cgcgaataga tgcgggggcc
      181 gcacgaggcg tcggcgccct cagggcgccg atcttctttc tggcgctcgc gcccctagcg
      241 ctgtttgccg cgggcctgcg cgtctggttc tgaagggcgc gcctcgccgc gagcagtgca
      301 aacacgcgga cgccagggcg cgggtattat ggttccggcc gctgcaaggc caagcccgca
      361 gcggtgaaac tttgcgactc gctgcttgtc tcgtagcagg gaaggacatg caaggttctc
      421 cgagaaccat agcgcggcgc tgggcgcgct ggctgatcgc gctcgccgcc atcggcttct
      481 gggtctggct gtttctagta cagctgacga tgccgcccat ggagcacagc ctcggcttcg
      541 acgcctggta cggaaactgg agggacgcgt tcatcgtgag cgcggtgttc ctcgcatttg
      601 tgctcgggtt cgcctggccg cgaggcggca tgcagtggcg caacgccggc atgtactcgg
      661 cgttcctgat atcgctcttc gtggagatgt tcggcatccc cctcacgatc tatgtcatcg
      721 cgccacttct cgatctccgg gcgctcgact tcggcatgaa cgagagccat ctctgggcct
      781 atgtcctcga ccgcgcgggc ctggtgccgc tcgccgccgg cgcgcgcctt gtcatgatcg
      841 tcagcatggc gctcatcgcc ctcggcgcag ccctggtcgc ggtgggctgg gcgcaggtgt
      901 acggcgcgcg agacgagctg cgaacctcgg ggctctaccg cctcatgcgc cacccccagt
      961 accttggcct gattctggtc gtcgtcgcgc tcgtcgttca gtggccgacg atcctgaccc
     1021 tggcgatggc acccctgctc gtcctcatgt acgtgcggca ggcgcgacgg gaggaccggg
     1081 acctcgaagc gcgctttgcc agcgcgttcg cggcgtacgc cgcgcgcgtg ccggcattca
     1141 ttccgcgcat gccgctctcg ccgcgcgagc gcgcttgacg ttatagtcgc tggaaggttt
     1201 atcgtctttg ccgcatggac tgcctcgaat tccgccggac ggtgctgtcc gacccgcgcc
     1261 ggcccggcgc cgtagccgag gcccatgccg ccggttgcgc ggcgtgccgg gagttcctga
     1321 cgcgaagtct ggaggatgaa gacgagctcg accgcgcgct gcacgtcgcg gtacccgaag
     1381 gcctgcagga tcgcgtcctc cggcgtgcgc gagcgacggg cgccagggtg cgctggctgg
     1441 cgcacgccgc gagcgtcgtg gtcgcggttg ccctcgccgt cgtctggccg cgcgacgatg
     1501 cgcttgccct cacgggcatc gatttcgtga tgttcgagga agcgcagtcc attgcggacg
     1561 ccgcaccgac ggactggaac gtgccggcag ccgcggcgcg cgacatgggc gtatcgctgc
     1621 cgcgagcgct tggcgagatg cgctgcgtct atcctctcgc cgccggtgcg gcgcaccacc
     1681 tcctggtcag gacgccgttc ggcaagctca cgctgctgtt gatccccgat cacgcgagcg
     1741 catcccgcgc ggccggcgcc gcttacgggt tcaacgccgc ggtgctgccg gcgggcaggg
     1801 gtacggtcgt catcatcggc gattcactga gaagcgtgca atgcgccgaa acgctgctga
     1861 aatcggcttg agctggtccg ccgtgcggcc ggtctccgcg ccagccaata gcaccgggac
     1921 ggcgtagcga gcatgccgga tcgtgccctc gcactgtcgc gctatcgccg tctcgcgcgc
     1981 ggctacgacg cggcgacgca cggcatcgag cgcaagcgcc ggcgcgcgat cgacttgctg
     2041 cggctgcggc cgggcgaaac cgtgctcgac gcggcctgcg gtaccgggtc ggcgcttgcg
     2101 ccgctggcgc gggcggtggg tcccggcgga cgggtcatcg gggtcgagca gagcgccgag
     2161 atgatcgcga ttgcgcgcca gagatgcgcc gcgctggggc tgggcaacgt ggtcctgctc
     2221 gaagcgcccg tggagaacgc tcgcatcgcg gggcctgtcg atgccgtgct cttcagcttc
     2281 gcgcacgacg tgctgcaatc gcccgcggcg ctggccaatg tctttgccgc ggcgcggccc
     2341 ggtgcgcgcg tggcatcggt cggggccaag ctctatccgc gctggctgtc gtttctcaac
     2401 ttctgggtgc gctggcgcat ccgcgactac gtgagcacgc ttgaggggct tgagcggccc
     2461 tggcgcctgc ttgcggagtt cgttcccgat ttcactgtcg cccgagtgac ctatcttggg
     2521 agcggctata tcggcggcgg cagctacccg gggatcgccg ccgggcgtgc tagtatcgcc
     2581 gccccgctat gcgcctgaca cgaacgctga ggatgtggat cgccgtcctg acgctgggcg
     2641 cgctcggctt cgcgcaggcg agcgtttcgc tcgcggcctg ctcgatggag cgcggcacga
     2701 tcagcgacgt cctggccgcg aagacctccg caccctgtga ctgtggcacc gcgatgacgg
     2761 agttcggccc gctctttgcg aaccgctgcg tggcccattg cacgtccggc ctgcagctgt
     2821 ccggcgccgc agccggcatc gcccacgtgg ccgccccggc tcctacactg gtgcggccgc
     2881 gagcgcagca gcgcgtcact tggcggcgcc gcgcgcagat gccgcctgtg gcgagcgttc
     2941 cgaagcgcat ccaattgcac tccttcctgg tctgattctc ccggccgccg ccaggcggcc
     3001 aaatgacgtt tcgtccttcg ggtcgcggcg cgggctgcga tccgcaacgt tgcaaggaga
     3061 atcggaatgc gctcgtctct gttcatcacg gcgtgctgcg cgctcgcggc ggcgccggcc
     3121 ctcgcccaat ccttgtcgct gcaagaggcg ctgcgcctcg gcgagaccca gtcgccgcgc
     3181 ctcagcgccc agcgcttcgc cgcgagcgca gcggccgagc aggtcggccg cgccggggag
     3241 ctgcccgacc cgaagttgcg cctcgggctg gagaacctcc ccgtaaccgg cgaggaccgg
     3301 ctgcgctacg accgggactt catgaccatg cgcagcgtcg gcctgatgca ggagttcccg
     3361 aacgccgaca agcgcgcggc gcgtaacgcc cgggcggtgc ggctgcacga gctcgagggc
     3421 gcgagtgtgc acgcgcaggt ctccgcgctg cggcgcgaga tcgccgtcgc ctggctcgat
     3481 gcgtactacg ccgagcgcac gcgcgaagcg cttgagcgtc gcggccggca gttccggctg
     3541 caggccgacg cgattgcggc ggccatcgca cgcggtcgcc agggagcagc tgacggcttc
     3601 atgctgcagc aggcgctcga gaccaatcgc ctgcgcgtga tcgagcagga gcgccagctc
     3661 gagcgcgcac gccttgcgct cgggatctgg atcggcgagg tcgcgcgccg cgagtttgcg
     3721 cccgcgccgg atacagcgcg ccttgccgag gccccggagc agatgctggc gcgtctcgag
     3781 cgccacccgg cgctgcgcac gtccgagcag cgcgaggcgc tggcgcgcgc ggaggtggac
     3841 ctcgcgcgca gcgcgaagaa ggcggattgg gcgctcgagc tcggctacgc gcagcgacgg
     3901 ccgtcgttcg acaacatggt gagcgtgatg gcgaccttcg agctgccctg gtgggcgggc
     3961 cgccgccagg atcgcgacgt cgcctcgcgc ctcgccgagc tcgagcaggc gcgcgcaatg
     4021 cgtgccgacg agcggcgcgt gcgagaggcc gagctgcgcg gttggattgc ggactacgac
     4081 gcggcgacgc ggcgcattga gcgtctggag cgcaccctgc tgccacttgc gcgcgagcgc
     4141 gccgcggccg ccgccgcggc cttccgcggc gcccggggcg agcttgcgcc ggtgctggag
     4201 tccgagggcg cggtaaccga aatggaggtc gagctggtcc aggcgcaggc cgagcgcggc
     4261 cgcgcctggg cgaacctgac gtatctcttc gcagaagaag gacagccatg aacaggacca
     4321 cgctgggcct tgccatcgcg actgcgcttg ccgcaggcgc ctacggcggc taccagatcg
     4381 cacaataccg cgccccggag acgcatgccg gcgcggcatc ggtaccccac ccggaggccg
     4441 caccggaaaa gcgcgtcctc tactggttcg atcccatgta tccgcagcac aagttcgacc
     4501 agcccggcaa gtcgcccttc atggacatgc agctcgtgcc caagtacgcc gagccggagc
     4561 gggatgcggg gacggtggtc atcaacccgc gcgtggtgca gaacctcggg gtgcgcaccg
     4621 ccgaggcggc gctcggcgcc ctcgagcggc gcttcgacgc cgtgggcacc gtggcgtgga
     4681 acgagcgcgc cgtggtgcag atgcaggcgc gtgcggcggg gttcgtggag cggctgcacg
     4741 cgcgtgcgcc cctcgatgcc gtgaagaagg gcgcgcccct cgtggaactg ctgatacccg
     4801 agtgggcggg tgcgcaggag gagtacctgg tgctgctcgc cagcgacgcc tccgaggcgc
     4861 gtgcgctcgc ccgcgccgcg cgccagcgcc tcgtgctgct cggcatgagc gaggccgata
     4921 tcgcggcgat cacgcgtgag cggcgcgtgc gcgagcgctt cacgctcgcc tcgcccatct
     4981 ccggcgtcat cgccgagctc ggggtgcgcg agggtgcggc ggtcgcaccg ggccagacgc
     5041 tgtaccgcct ggtggacctt tccaccgtgt gggtgaacgc cgagattccc gaggcgcagg
     5101 cggggctggt ggtgccgggc agcagggtcg aggcgcgcgt gcctgcctac cccgaccacc
     5161 tgttcggcgg caaggtcggc gcgatcttgc cggaggtgag cgcagccacg cgcaccgtgc
     5221 gcacgcgcat cgagctggac aaccatgagg gcctgctcaa gcccggcatg ttcgccacgc
     5281 tgcgtttttc ctccggcaat ggcagggagc aggtgctcgt accgagcgaa gcgctcatac
     5341 gtaccggaac acgcagcgtg gtgatcctcg cgctcggcga gggacacttt cgcgccgcgg
     5401 acgtcgaggt cggccaggag ggtggcgggc gctcggagat cctgaagggg ctgaagccgg
     5461 gcgagcgggt cgtgctctcc agccagttcc tgatcgactc ggaggcaagc ctgtcggccg
     5521 cgaccgcgcg actgcaggag ggcgacgagc ccgctcccgt ggcggccagt gaccggcaca
     5581 ccggccgcgg caaggtcacc gggctggaga tcgccggagc acgcgtcgag ctcgatcacg
     5641 ggccgatccc cgccctgcag tggccggcga tgcgcatgga gttcaagctc gccgatccga
     5701 agcagctcga cgggctcgcg cagggcgatg aggtgcagtt cgaactacgc ggcgagccgg
     5761 acaaggacgg cgactacgtc atcgagcgca tcacgcgcag gcccggtcga tgatcgccgc
     5821 actgatccgc tggtcggtgc acaaccgcta cctcgtcatc ctggcgacga tcctcgtcgc
     5881 cgcgtggggc gtcgtgtcgg tcctgcgcac gccgctcgac gcgatcccgg acctgtcgga
     5941 cacgcaggtc atcatccgca cgccgtttcc cggccaggcg ccgcgcatcg tcgaggacca
     6001 ggtcacctat cccctcgcca ccacgatgat gtcggtacct ggcgcgaaga ccgtgcgcgg
     6061 ctactcgatg ttcggggatt cgtacgtgta cgtgctgttc gaggacggca ccgacctgta
     6121 ctgggcgcgc tcgcgtgtgc tcgagtacct gaaccaggtg caggggcggc tgccggcctc
     6181 ggccaagccc gcgctcgggc ccgacgccac cggcgtgggc tggatttatc aatacgcgct
     6241 cgtcgatcgc agcggccgcc acgatctggg gcagctgcgc gcgctccagg actggtttct
     6301 caagtacgag ctgaagagcg tacccgacgt cgccgaggtg gcgtcggtgg gcggcatggt
     6361 gcgccagtac cagatcgtgc tgttcccgga acggctgcgc gcctacggca tctcgcacaa
     6421 ccgcgtgatc gatgccgtgc agcgcgccaa tcgcgagacc ggcggctcga tcctggagct
     6481 cggcgaagcc gagtacatgg tgcgctcggg cggctacctg aagtcgctgg aggacttcgc
     6541 cgccgtgccg ctcggcgtaa gcgacggcgg aacgccgatc ctgctgcgcg acgtggcgcg
     6601 catccagatg ggaccggaga tgcgtcgcgg cgtatccgag ctcgacggcg agggcgaggc
     6661 ggtaggcggc atcatcatca tgcgccacgg gcgcaacgcg ctcgagacca tcgccgcggt
     6721 caagaggaag ctcgcatcgc tcaagcccgc gctgcccccg ggcgtcgaaa tcgtggaaac
     6781 ctacgatcgc tccgggctca tcgtgcgcgc ggtgcgccat ctggcggaga agcttgccga
     6841 ggagttcgtc gtcgtcgcgc tggtgtgtct cgcgttcctg ctgcacctgc gcagctcgct
     6901 cgtcgccatc gtcacgctgc cgctgggcgt gctggcggcg ttcatcgtca tgcgcttcca
     6961 aggcgtcaac gccaacatca tgtcgcttgg cgggatcgcc atcgccatcg gcgcgatggt
     7021 ggacgccgcc atcgtcatga tcgagaatgc gcacaagcgc ctcgaggcgt ggcggcacgc
     7081 gaacccggaa ggggagctct ccggcgcggc gcgctggcgc ctgatcgagg aggcggctgc
     7141 cgaggtcggc ccggcgctct ttttctcgct gctcatcatc acgctgtcgt tcatccccgt
     7201 gttcgcgctc gaggcgcaag aagggcgcct gttctcgccg ctcgccttca ccaagaccta
     7261 cgccatggcg gcgtcggcga tcctctcggt aacgttggtg ccggtgctga tgggctacct
     7321 gatccgcggg cgcatcccgg acgagcggcg caatcccctg aaccgcctgc tcatcaccgc
     7381 ctaccggccg ctgctcgcgg ccgtgctgcg cgccccgcgc acaacgctcg tcgtggccgc
     7441 actggtggcg gcttcgacgc tgtggccgat cgcgcgtacc ggcacggaat tcattccgcc
     7501 gctcgacgag ggcgatctgc tctacatgcc caccgcgttg ccgggcctgt cgcccggcaa
     7561 ggcggcgcag ctgctgcagc agaccgacaa gctgatcgcg acggttccgg aggtcgagcg
     7621 cgtcttcggc aagatcgggc gcgccgacac cgcgaccgat cccgccccgc tcgagatggt
     7681 cgagaccatc gtgcggctca agccgcgcgc ggagtggcgg cccggcatga cgcccgagaa
     7741 gctcatcgag gagctcgacc gcctggtgca gttcccgggg cttgccaacc tgtggatccc
     7801 gccgatccgc aaccgcatcg acatgctcgc caccgggatc aagagcccgc tcggcatcaa
     7861 ggtgtcgggc gacgacctgg gcgcgatcga gggtgccgcg gcgagcatcg agcgcgcggt
     7921 gaagacggtg ccgggcgtca cctcggcgct cgccgagcgc gtgcgcggcg ggcgctacat
     7981 cgagatcggc atcgaccgcg ccgccgcggc acgctacggg ctgaacatcg ccgacgtgca
     8041 gtcggtggtg tctgccgcga tcggcgggga gaacatcggc gagacgatcg agggccgcca
     8101 gcgctttccc atcaacgtgc gctacgcgcg cgaggtgcgc gactcggtgg acaagatccg
     8161 caacctgccc atcgtcaccg agcgcggcgc acagatcccg ctcgccgcgg tggcgagcgt
     8221 ggcgatcgcc gacggcccgc cgatgctgcg ctcggagaac gcgcgcctga acggctggat
     8281 ctacgtcgac atccgcgggc gcgacctcgg ctcggtggtg gccgacgcgc agcgtgccgt
     8341 ggcgcgcgag gtgaaactgc caccgggggt ctcggtggcg tggtccgggc agttcgagtt
     8401 cttggagcgc gcccgggcgc gcctgacgct ggtggtgccc ttcgtcctgc tgatcatcct
     8461 cgtgctgctc tacatgacgt tccagcgcat ggacgaggcg ctgctcatca tggtgtcgct
     8521 gcctttcgcg ctcgtcggcg ggttctggac gctttatgcc cttggccaca acctgtcggt
     8581 ggcgagcgcg gtgggcatga tcgcgctcgc cggcgtcgcg gcggagttcg gcgtcgtgat
     8641 gctgctctat ctcaagcagg cgatcgagcg gcgggaggca gcgccggcgc acggcgggct
     8701 tctcgaagcg ctcgacgaag gtgccgtgca gcgcgtgcgc ccgaaagcca tgacggtggc
     8761 ggtgattctc gcgggcctcg caccgatcat gtggtcggag ggcaccgggg cagaggtgat
     8821 gcagcgcatc gccgcgccca tgatcggggg aatgatcacc gcgccgctac tgtccatgtt
     8881 cgtgataccc gcggcgtact acctgatgcg gaggggcaag gcatgaagcg acggcgattg
     8941 ccggcgatgc tcaaggcgat ggccaacgcg ggagcgcagg ccgcgagcga cgccccgccg
     9001 aaactgcgca ggctctttgc gtagacacgg cgattcaggg ccgcagcgtg atcggcgcga
     9061 gcgcctcgcc ttgcggcagg acgcggccga tgaccgcggc ccggggatag ccgtcggcgc
     9121 gcagcgcctt caggcagtcc tgcgcctggg cggcgggcac gctcgcgagc aggccgcccg
     9181 cggtctgcgg atcgaacagc agcggatagc gcgggtggct caccatctgc tcctggttgc
     9241 tgagcgcgcg acgcagccga acattggccg gctggagcga gctcagaata ccggcggcgg
     9301 ccatttcctc ggcgccctcg agcaccggca gcgccgagag ctcgatctcg gcgtccaccg
     9361 ccgaaggccg ggtcatttcg acgagatgcc cgagcagccc gaagcccgtg acgtcggtgc
     9421 acgcggtcgc gccgtgccgc agcaggcatt gcgcgcccag ccggttcgac gtggtcatgc
     9481 tcgcgagcgc ggcatcgatc cagcgcccac gcgcggcgag cttggcgtgc gcggcgagca
     9541 gcgtcccggt gccgatcggc ttggtgagga tgagcacgtc gccagcgcgc atgccgcctt
     9601 tgcgcagaac gcgtgcctcg tcgatcagtc cgttgacggc gaagccgagc gcgagctcgc
     9661 gtccttcgcc gctatggccg ccgaccagcg cgcagttcgc ctcgttgagc acttcgatgg
     9721 caccggacat catctggcgc agcacgtctt cgaccttgct ttccagtccc gccggcacgg
     9781 tggcgattgc ggtggccgac tgcgggtccg cgcccatggc gaagatgtcg ccgagcgcat
     9841 ggttggcggc caccttgccg aagaggtacg gatcgtcgag gaaagcgcgg aagaaatcga
     9901 ccgtgtggac gagcgccttt cccggcggca cgcgtaccac cgcggcgtcg tcgggcgcgt
     9961 gcagtccgat gagcacttcg gcgcgcggca ccggttgcag cgaaccgagg gcgcgcgaga
    10021 gcaccgtcgc gccgaccttc gcaccgcaac cgccgcagcg catggcgatg gcggaaatcg
    10081 cctgcgcggc ctcttctgcg tcaagccgca ccgcaggcgg cgcgcccgcg ggaggcgcgc
    10141 tcatctcggg gagctcgccg aaccggcgca tgaagcgccg gtcgatccag tccttccagc
    10201 gccaccccca gtcgccccgc aggtacagca ggccgcgcga cgccaccgcg ctcttgtcgc
    10261 ccgtgctaat gagcgcgagc cagcgccgct gcgggcggta acgcgcgagc tgccggccgg
    10321 tcaccgcgcg acgcagattc tcggcgagcg gcggcccctg ccggaccgcg aagacgccgg
    10381 ccttctcgag cgggtgatcg accatgctgg caatgtcgcc cgcggcgaag atgtccgggt
    10441 cggtgagcgt ctgcagcgtc tcggccacgc ggatgaatcc gtccgcgtcc agctcgagcc
    10501 ccgtatcgcg cagccagcgc gccccgcacg cgcgcgtgac ccagacgatc tcgtcgacgt
    10561 cgagggcgtc gccgcgcgac gtgcgcagcc gtccgggcga cacctggacg acgccggcat
    10621 cggtgtgcat cacgacgccg cgcgcgcgca gcacgcgctc gaaggtccgg cgtaccgcgg
    10681 cgttgtgcgt cggcaggata cggggtccgg cgtcgagcag gtggaactcg atgtcgccgg
    10741 gatcccggcc gcgcgcgcgc aactcgtgtc tcagccggtg ctgcatggcg agcgcgagct
    10801 cgacgccgcc cgcgccggcg ccgaccacgg cgatgcgcgc cttgcccggg tgcgcgcgta
    10861 cgcgctcgag cagcgccagc cagcgcgcgt tgaaatcgtg gatcggcttg accggcacgg
    10921 cgtgttccgc ggccccgggc acgtcctgca cccgaggcgt cgagccgatg ttgatggaca
    10981 gccggtcgta ggtcacgggc ggccggctcc tgcagatcgc cttgcgcgcc ccgcgatcga
    11041 gcgccaccac ctcgtcgcgg aagaaccgcg cgcccgcgaa ctgcgcgagc cgcgcgaggt
    11101 cgatatggac ctcgtcgtac gcgtaatgcc cggcaatgta acccgggagc atgccggaat
    11161 acggcgtgtg cgtatcgcgg cagatgaggg taaggcgcac gcccggcatc ggcttcatgc
    11221 cgaagcgccg caggacgccg acgtggctgt gaccgccgcc gaccagcacg atatctctca
    11281 atacgggctg gtcaggcgtg agcatgcgtg cggcgggagc gaggcatgat gttaaacacc
    11341 ggaatctggc ggccagggcg cgaaagcgct gctgacgcac ggcgccatac tacaggcgaa
    11401 atgccgccgg gaagcgggac gcccgcggct tgactcgcgc ttgttggtgg cgctaccgca
    11461 agatggcgcg gaacctgcga agcgatcctg agcgtgaggc gacttgccta ggcgcagcta
    11521 tcgcccatct gcttgggcgg acaggcgacg ctgccgtaac tgcagaacac gcagcagtcg
    11581 cccggcttgg gcttgatgcg cgtcgcgcac gccgggcagg accagaagaa caggcaggcg
    11641 tctgacggca tgacctcgct cgagcgcccg ccgcacaccg ggcacgtgag cgtgctctcg
    11701 agtaccgcgg cgctcacggc gccctcggcg cggccgggta gccagcgtcg ctcacggcct
    11761 gcgcgagccg cgcgggcgag gttttctccg gatcgaagac gacctgcgcg gtcttggtgg
    11821 cgagatccgc tctgacctcg tgcacgccgg gctgcttgcg cagcacaccg gacaccgcga
    11881 tcgggcacag cgagcaattc atgctcggca catccagcgt gacacgcgcg ggctcggcgg
    11941 ccatcgccgc actcgcaagc gccagcgcgc tagccaagaa gatacggaac atagaagggc
    12001 gcaagcagca gcgccttggc ggcgacgagg gtggcccaga acgcgatgcg ctggttgcgg
    12061 cgcgacgcgg gcgtgcaggc cagctccggc ccgcaggtcg tgggacgcag gtagagccgg
    12121 tagaacgcgt aaccgaacgc cgcggcggcg aggccgatga acacccactg gaactgctcc
    12181 agcgcacgca gctgcgcgat ccaggcgccg ccgaagccca tcgacaccag gaccagcggc
    12241 aggacgcagc aggtcgaggc gccaagcgcc gaggcggcgc cggcggcgat ggaggcggtt
    12301 tcggcggctg gtttctgcac gcgggaagtc tatagccgtc cctagatacg gggttaaacg
    12361 gagctggggg ttctgtgtcc gagattacca tcggcaggct ggcgcggcgt gctcaaggca
    12421 ctcatcggtc gctgcgaccg gcgccgcggc aaggtcgcct gcccgatcat cgagagcctg
    12481 gcgcgaccgg cctgagaagg ccgttcgcgc tcgccgtcgt gcgcagccgc tcgcattgcg
    12541 catcgtcaag gtgaaccttg cccggcgccg tatcctcgcg catgaggttg tccggcgccg
    12601 ccgagtgctc gccgctgtcc atcagcacgt gcgccagctc gtgcgcaagc acgacatcga
    12661 ggtcgcgcgc gccgcgcgtt atccagaccg tatcgcgcag ctcgggccgc gaggcgctgt
    12721 tgccgcggcc gatcgcctcg gcctcgaacg ccggacgctg gcgtgtctcg gcgacgaagt
    12781 agatcgtcgg ccggggcaac gccagcgcgc gcgccagctc gcgcgatgcc ggcacatgaa
    12841 agtcgcggta gcgggccggc gcgccgatca gcgccagttc cgctttcgcg atcgccacgc
    12901 cgcactgtgc gaggatggcg gtggtcttgc gcagcgccgt atggatgcgc gctggctccc
    12961 agctaccctt gaaataggcg agcaccaagc tgaggcggtg cgtgtgcgcc ggcgtgccgc
    13021 gctcgagcgc gagctcgcgc cgctcgagca cggagatcga atccccgctc catgccggcg
    13081 cagccaaggc aggaagcagc agcacaagca gcgttcgcgg caaagggctc acggcctagt
    13141 gcccgtggcg gtggtgcaga tcggggtagt gcggatgcga atgcaccagc ggctcgtgcc
    13201 ggtgcggatg cacgtgcggc tcgcggccgt cccaggggaa atcatgggtg tgccggtggt
    13261 gcgcgtcgtg cacgtgctcg tgcgtgtgcg ccatcgcctc gtgggaatgc aggtgctcgt
    13321 gccgctccga cagatgcagc catacgccga gcgccatcag gcccgcggcc agccagaacg
    13381 ccatcccggg gcgctcgccc agcaccgcga tggctaggac cgcaccgaag aacggcgcca
    13441 cggagaagta cgcgccggtg cgcgcggtgc ccagcccgcg cagtgcgacg atgaacagca
    13501 cgaggctcac gccgtagccg aggaaaccga ggagtccggc ggcgagccag cccgataggg
    13561 cggggaacgg cgcgccgaga acccaggcga gcgcgaggtt caccgcgccc gcggccagcc
    13621 ccttcaggca ggcgagcgtc gccgcgtcgc cgccggagac acggcgcgtg aggttgttgt
    13681 cgatcgccca ggcgaggcag gcggcggtga cgaggagcac gcccgcgaag ctcccggcct
    13741 gcggcgcggc acccgtggac aacaacgcac cgcccgccac gatcgccatc atgcccgcaa
    13801 agacgcggcg atccacgttc tcgcggaata ccgtccaggc gatggccgcg gtcagcaccg
    13861 cctcgaggtt gagaagcagc gacgcgcccg atgcgtcggt gcgggccagc ccttgcatca
    13921 gcaggatcgg tccggcgacc ccgcccgccg cgatcgccgc cgcaacccag ggccagtcgg
    13981 cgctcggaat cggcgcgacg ggctcccgag gcgacaggcg cgagcgcagc gcgacccatg
    14041 ccgcgaggcc gatgccgctg cccaggtaca ggaccgccgc gagcacccaa ggcgacaccg
    14101 cccccaggag cagcttcgca aatggggtgc tcgcgccgaa aagcgccgcc gcccccagtg
    14161 cgtagagcgc tccgcgactc atgctttgca atgatgccgc atgagctcag gcgtgcctga
    14221 atgcctagct ggatccgcgg acgtggctgt tgttgcgcgc gcgccgcgcg gcgggacgcg
    14281 agagtgacct gacgatgccg caggcgcgcg cccctcgccg ccccgcacag tgcgcgcgga
    14341 tggcgtcgag ctcctgcttc agcgcggtga ggtcggcgat gcgcgcggcc acgtgggcga
    14401 tgtgctcgtc gagcagcgcg tccaccggcg cgcagtccgc gcccgggcgg cccggcgccg
    14461 cgagcaggcc ctccttctcg tagtagcgga ctgtctctac gggacagtcg gcgcgcttgg
    14521 cgagctctcc gatgcgcatt gcccctgcag tatctcacgc cgtgcctggc gcacgacgct
    14581 gtaggaggag gtcaacgcga gcgacgccag cagcgccgct acgatccaat cgggccagcc
    14641 agtgccactg ccgagcacgc cggcggcggc ggcgattacg gcgacgttgg cgatggcatc
    14701 gttgcgcgag cagagccaca ccgagcgcat gtcggcgtcg ccggtgcggt atcgatagag
    14761 ggacgccgcg acgccgacgt tgaccaccag cgcgagcagc gcgacgaccc ccatggtggt
    14821 ggcgtcaggc agcgtgccgc gctgtgcgtg ccatgcggtg atcgcaagca ccagcacgcc
    14881 gtaggcgccc atgcacaggc ccttgaagta cgccacgcgc ggccgccatg cgggcaccag
    14941 cagcagggcc ccgagcgaga gcgcgtaatt ggacgcatcg cccgcgaagt cgaccgcgtc
    15001 ggcaagcagc gacgacgagc tggcggcgat gcccgagacc agctcgacga ggaacatgag
    15061 cgcgttgagc gccagcgcga ccgcgagcac gcgccggtat gcgggatcgg cggcgtgcgc
    15121 ttgggcgggg cagcaggcgg cgctcatggg ccacattaca aaccctggac cagctacagg
    15181 gtcaagcggg cgccgctacg cggcgcgacg cacgcgcttg cgcgcattgc gtgtgcccgt
    15241 ggcgcggcgc ttgcgcgccc ccgcgggcag tttcaccacc cgggtctcgc gcggcataac
    15301 cgtctcgagc ggcaggtgca gccgccagaa accgcgctcg cggccgttgc gccagaatcc
    15361 ctccaggatc gtgctgtcgg cgaaacggtg caggcttgcg tcgatgcccg cattcggtgc
    15421 gcccaggaac tttataagtg gttgaattat agcaattaac ggatcgttcg gcaatccgcg
    15481 ccgcgcgctg cgcacaacgc ttgaggaacg cgtcgtggtg ccgattagac tgcaccgcac
    15541 cggcctctca ccgggccggt tacggcggtt gacgttgacg taaacgtcac ataggctgcc
    15601 gcccactgga gcccgcatga ccgacctttc ggaatccaag agcctcgtct acaaggccaa
    15661 gcacaagatt cgcttcgtca ccgcggcgag cctgttcgac ggccacgacg cctcgatcaa
    15721 catcatgcgc cgcatcctgc aggcctcggg cgcggaggtg atccacc
//
LOCUS       DBNHKE010000200        14923 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1147005, whole genome shotgun sequence.
ACCESSION   DBNHKE010000200 DBNHKE010000000
VERSION     DBNHKE010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1147005"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="AC16O9_13710"
     CDS             complement(<1..420)
                     /locus_tag="AC16O9_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6517706.1"
                     /translation="MTMILRCMAVLCVALSLSLPARATEEDASALAELARPGGILLMR
                     HAIAPGFGDPAAFRLGDCATQRNLDATGREQARALGARLRAAGIRRAKVYSSQWCRCL
                     ETARLLGIGEVEPLPALNSFFDRPQERERKLAAVRAFL"
     gene            complement(541..1764)
                     /locus_tag="AC16O9_13715"
     CDS             complement(541..1764)
                     /locus_tag="AC16O9_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HIR6517707.1"
                     /translation="MPLEKPAVAPPSLRQTLADFGALYAANALVAFIFAASGPVAILL
                     AVGMRGGLSESDLASWLFGAFFVNGLISIAFCWFYRQPLVFFWSIPGAVLVGPALGHL
                     GFREIVGAYLACGVLMLVLGLSGWVKRFMDAVPMPIVMGMVAGVFLRFGLDLVFAIRD
                     AFWIAAPMTVAFLALSASPRLSRALPPVIGALVVGIFMIAVLGQFEARGAEAFAIVRP
                     NLYLPAFSWQAMLELVVPIAITVLVVQNGQGIAVLRAAEHQPPINAITAACGVGALVS
                     GMVGTVSTCLTGPVNAIITASGEKHRHYTAGIMVGVLALGFGLLSPVFTRLMLSAPKA
                     FIAALAGLAMLRVLQTAFSISFSGKFALGALVTFLVTVADVPILNVGAPFWGLVFGLA
                     ASWLLERGAFSGPAG"
     gene            complement(1798..2505)
                     /locus_tag="AC16O9_13720"
                     /pseudo
     CDS             complement(1798..2505)
                     /locus_tag="AC16O9_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
     gene            2668..3879
                     /locus_tag="AC16O9_13725"
     CDS             2668..3879
                     /locus_tag="AC16O9_13725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR6517708.1"
                     /translation="MNRTDPHFATPLRIPGDRFLHAPGPTHIPAEVLAAMHRQPMDHG
                     DPRLDQIIAVCEAGLRRLLRTEKADLFMYAANGHGVWEATTDNLLDLGDAALIPGSGH
                     FSEQWALQAEATGRRALRTPYKEGYPIDPNDIERALRADKKGEIAAVFVVHTDTASGI
                     TNDLQAIRAAIDAAGHPALLVADVVASLAAAPFAMDALGVNVAVGASQKGLMSPPGLG
                     FIAVDARAAQVAAKNPAPRHYWDWKRRKGDYSYTKFCGTPPQNLLYGIEAALALLDRE
                     GMDAVLARHALLARAVQTAVECWAQEGALFLHCRVPAARSVSVTTIEARPGLDVEALR
                     SVARERFQVAFAGGLGPLQGRAFRIGHLGDVNAAMILGCLAGVEAAMRVQGTPFGRDG
                     VAKAVECLAAS"
     gene            complement(3887..5077)
                     /locus_tag="AC16O9_13730"
     CDS             complement(3887..5077)
                     /locus_tag="AC16O9_13730"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HIR6517709.1"
                     /translation="MGADPRDISLARGLLIAFLLSFGPAVSNSFSRFAYALILPAMRS
                     ELEWSYSQAGSINTVNALGYLVGALLTRALVARLGNRLLYGWGMVITSVAIAATGFVR
                     DFDALMAVRALAGVGGAAVFICGGALSGNIFPKRPQLATTTIAIYFAGGGLGLMLSGI
                     ALPLLLEARGSASWPLAWRGMGYASLLLTGAAAWAALRIAEPGRMPGEARWEVRPFAA
                     ELASYALFALGYIGYMTFVIAWMRVNGASTASVIAVWFVLGLATLLAPLVWRKPAEHW
                     PGGRPLAAVMAVLTAGAALPLVSTHPAAMLLSAALFGLAMFSAPSSVGSFIKRALPSP
                     AWGSAIATFTVVFAAGQIAGPVAIGWLADRSGSLQVGLAASAALLAVGAVLALAQRDL
                     RLAP"
     gene            complement(5082..6317)
                     /locus_tag="AC16O9_13735"
     CDS             complement(5082..6317)
                     /locus_tag="AC16O9_13735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517710.1"
                     /translation="MPRAPTLAPFGVRSFRFQWPADLATSWAFEMEALILGWFVLSTT
                     GSVEKLVLVAALAWLGSLFSPFFGVAGDRIGLRALLCITRGAYALLAAVLALLTLTGA
                     LAAWHVFVIAALAGLMRPSDMAMRNVLVGQTMRPEILMGALGISRTTSDTARIAGALA
                     GTGGVALIGMGPAYVVVTAIYVAAFLLSLGVAGTPVRGKTETAAVLAGLQQAIGYIRG
                     KPDLLGALGMAFLVNLLAYPFFLGLLPYVAKDVYGIGQAGLGYLAASFATGALAGSVL
                     LSVTRLPLRAGRIMMWSGAAWFTAVVLFGQIPFISVGLLLLFLSGLVQSFCMTPLAAV
                     MLRASSEEMRGRVMGIRMLAIWGLPLGLLAAGPIIAGIGYGATTLIYGGIGLAATLVI
                     GYRLRSALWHHSAAANAHL"
     gene            complement(6336..7109)
                     /locus_tag="AC16O9_13740"
     CDS             complement(6336..7109)
                     /locus_tag="AC16O9_13740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517711.1"
                     /translation="MSGLAVVTGAGSGLGQAIAKKLAADGFAVAAIDLDEAAAKQTAR
                     QIGGKAAAYRADVSRAAELDKAVSAAIKELGTLEVMVNNAGILDGYFDVDEMDEALWR
                     RVIEIDLTGVFLGSKRALQEMLPRKRGRIINMSSVAGLNGTGGGAAYIAAKHGVVGLT
                     RQMAVQYGARGIRANCVCPGAIPTGLRAHSQAILGPGVPDMSGRGVAVNDEMVKALVP
                     LGVRGTPEDVADAVSFLASDAAHYVNGHALVVDGGWRAK"
     gene            complement(7106..7990)
                     /locus_tag="AC16O9_13745"
     CDS             complement(7106..7990)
                     /locus_tag="AC16O9_13745"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6517712.1"
                     /translation="MEKIRIQFTLFSAFYSPLISAMSGGFLRAEGLDPEWSVSPPGVS
                     ALQSLKDGSAHVVQSALSQGFVTLDKGETPSAVHFAQVNEMDGFFLTARKPDAAFSWA
                     RLEGAELVTFKGGQPAAMFKYACHKANIDYSKIKLVTPGGPADIDKAFREGQGQYVHQ
                     QGPFPQQLQADGVGHVVAQVGPQIGPCGFSSLAATREWLKSDMAKAFMRAYRKTRAYM
                     NDAPAAQIAKAEKPFFPKIDEAVLADCIATYQKLGCWTRHVEITRAAYEKTLDVFEFN
                     GQLKRRYRYEQVCAEPPA"
     gene            complement(7994..8314)
                     /locus_tag="AC16O9_13750"
     CDS             complement(7994..8314)
                     /locus_tag="AC16O9_13750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6517713.1"
                     /translation="MKPQPYKVRNIHVVAKSDDMLVREYTLDPGDTIPWHRHSQVADY
                     YYGLEGTVVVESRNPVARHDLGAGQSASVTPGTVHQVSNSAKSRCRFLLIQGVGKYDF
                     VREE"
     gene            complement(8330..8563)
                     /locus_tag="AC16O9_13755"
     CDS             complement(8330..8563)
                     /locus_tag="AC16O9_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517714.1"
                     /translation="MNLFRSKEHVKKWSGFKPGTEAGILTLADAMRVMSTPRHRDRLR
                     PDYVSTVQESVPAFVQRLLEVSHDSPHWDLRPD"
     gene            complement(8570..9034)
                     /gene="merB"
                     /locus_tag="AC16O9_13760"
     CDS             complement(8570..9034)
                     /gene="merB"
                     /locus_tag="AC16O9_13760"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HIR6517715.1"
                     /translation="MAAAQLDQTYHYILSTLVARGQAPHFTEIAAKFGIPAEQGRRRL
                     RELMAERLPNWLFPDTDLIASFAPFNNLPTQYRISVDGEQKWFAQCGLESLAMTWLFP
                     GKRVTVSAPCLDCGEPIRVEIVDGEIERAEPGDIHFYVELPIKKWFANLPFA"
     gene            complement(9098..9442)
                     /locus_tag="AC16O9_13765"
     CDS             complement(9098..9442)
                     /locus_tag="AC16O9_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517716.1"
                     /translation="MYMMSAVAALCAVALVAGLVREACRHRPDLWITSDDAILCVVSP
                     LLILLATCGAVAVGYRLTHGGLAAVAVGAWIGSAAIAVAAALIGIFLSARLRKRAPRP
                     DGGLISKPLAAR"
     gene            complement(9442..9648)
                     /locus_tag="AC16O9_13770"
     CDS             complement(9442..9648)
                     /locus_tag="AC16O9_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517717.1"
                     /translation="MVLTSAALVASGVACLFGSGYTLYTVSPKEGRPASFWTRTEARS
                     TMLALAIVTAFVIGGGLVLKGILS"
     gene            complement(9738..10445)
                     /locus_tag="AC16O9_13775"
     CDS             complement(9738..10445)
                     /locus_tag="AC16O9_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6517718.1"
                     /translation="MSESRPRVLVVDDEPQIRKFLRFGLEGHGYAVHEAGSAAAALSA
                     AAAAPPDLVVLDLGLPDGDGFDVLARLREWSSVPVIVLSVRSREAEKVRAFELGADDY
                     VVKPFGMPELLARIKAALRRRVEAAAPAPVFRVGALEVDLVRRVVRVSGAPVRLSPKQ
                     YQLLQILVTNAGKVVTHRQLLNDIWGAAHREDVQYLRVFVRKLRSRIEVDPARPVYLQ
                     TELGVGYRLRTPDQLPP"
     gene            complement(10442..11488)
                     /locus_tag="AC16O9_13780"
     CDS             complement(10442..11488)
                     /locus_tag="AC16O9_13780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6517719.1"
                     /translation="MRGRLALLAYLKSALAVAGVGLAAHALTRLVPLPHVSVLFVAAV
                     ITSAALWGLGPSLFAAFLSVAAGSYLFYSPVYSFHVTDPQDFADLAVFIIVAVLTSRL
                     ADGVRRQAREIADARLKAQGEALREALINSMSHDLQTPLAAILGSASVLETLGDAGEP
                     RVRRELAATIREEAERLASHIGNVLDLTRVRAGQIAPRLELVELSDIINGALRRKDKA
                     LAGHTLQVDVPAGLPMLRLDLFLMEHALANVLDNAAKFSPAGSTVSISARPANGGVLL
                     EIADAGRGIRADELDRVFEAFYRGSPQEGGAAAGSGLGLAICRAFVEANGGSVTLASA
                     GPGRGTRVQIRLPA"
     gene            complement(11496..13613)
                     /locus_tag="AC16O9_13785"
     CDS             complement(11496..13613)
                     /locus_tag="AC16O9_13785"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR6517720.1"
                     /translation="MAAAVQSLDASRFGYLKKADIARLADAYRFSEAAHAGQKRQSGD
                     PYISHPLAVAEILAGWRLDGQALMAALLHDVMEDTAVTKAEISDTFGTPVAELVDGLS
                     KLDKIEFQSAEDAQAENFRKMLLAMARDVRIILIKLADRLHNMRTLDAVPPAKRRRVA
                     RETMEIYAPIANRLGLNALYHELQELAFAHLYPLRYRTLVKATKAARGNRREVVGKIE
                     SAVNKRLTDAGIAAAVSGREKHIYSIYRKMMEKHLSFSEVHDIYGLRVVVKDVATCYL
                     ALGTLHALYKPVPGKFKDYIAIPKANGYQSLHTALIGPYGVPLEVQIRTEQMHHLAEA
                     GVASHWMYKDDAVSLSDLQKKTHQWLQSLLEIQNQSGDPHEFLETVKVDLFPDEVYVF
                     TPAGKILSLPRGATAVDFAYQVHTDIGNRCVAAKVNGELVPLRTELRNGERVEIITAS
                     HAKPNPAWLQYVRTGKARANIRHFLKTMQYEESAALGERLLEQALNGYAVAITDIDDA
                     SWERVIRETVGRTKRELLADIGLGKQLPAVVARRLVMRAESDAKPVSKAVSVVIRGTE
                     GMAVQLASCCRPIPGDGIVGSMKKGQGLVVHNADCRVIERSRKNEPEQWIDVEWAPAT
                     TRLYQAAIKVMVANQRGVLAKVASEIAEAGANIDSISMEDDRAFFTNMHLVLEVSNRG
                     HLARVMRALRRVPDVKKLARVRE"
     gene            complement(13664..13891)
                     /gene="rpoZ"
                     /locus_tag="AC16O9_13790"
     CDS             complement(13664..13891)
                     /gene="rpoZ"
                     /locus_tag="AC16O9_13790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR6517721.1"
                     /translation="MARITVDDCLQRIPNRFQLTLAATYRARQLGAGASPLVEPNRDK
                     VTVIALREIATGKVGTEVLKRNPIVPTPPAP"
     gene            complement(13894..14502)
                     /gene="gmk"
                     /locus_tag="AC16O9_13795"
     CDS             complement(13894..14502)
                     /gene="gmk"
                     /locus_tag="AC16O9_13795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR6517722.1"
                     /translation="MNGLLFVVTAPSGAGKSSLIKGLLAAERGIALSVSYTTRPPRPG
                     EADGREYHFVGLREFEAMLERGEFLESAQVHGHRYGTSQKVIEKARASGLDLMLEIDW
                     QGAMQVRRLHSDAIGVFILPPSMGELERRLRARAQDADAVIQRRLASAADEMTHAVEF
                     KYAIINNNFDDALLDLRAVVRAERLAVARQLARHPEVFRPEP"
     gene            complement(14499..>14923)
                     /locus_tag="AC16O9_13800"
     CDS             complement(14499..>14923)
                     /locus_tag="AC16O9_13800"
                     /inference="COORDINATES: protein motif:HMM:NF019940.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endoribonuclease YicC domain-containing protein"
                     /protein_id="HIR6517723.1"
                     /translation="EGAKLASILGERVAEMRKRLAPIAPLVPEANAAYQARLAERLRE
                     ALGAADDERLRAELAVFAVKTDVEEELQRLGTHITEAERVLAAGGAAGKRLDFLAQEL
                     NREANTLASKAATREIADCALELKLLIEQVREQVQNIE"
BASE COUNT         2367 a         5213 c         5241 g         2102 t
ORIGIN      
        1 aagaaacgcg cgtacggctg cgagctttcg ctcccgctcc tgcgggcggt cgaagaacga
       61 gttcagcgcg ggcagcggct cgacctcgcc gatgccaagc aggcgggcag tctccaggca
      121 gcggcaccac tggctcgaat agaccttggc gcggcgaatg cccgccgcgc gcagccgcgc
      181 gccgagcgca cgcgcctgct cgcgcccggt ggcatcgaga ttgcgctgtg tcgcgcagtc
      241 gccgagcctg aacgccgccg gatcgccgaa gccgggcgcg atggcgtggc gcatgagcag
      301 aatgccgccg ggccgggcga gctcagcgag ggcgcttgcg tcttcctccg tggcccgcgc
      361 gggcaaggag agcgaaaggg ctacgcacag cacggccatg cagcgcagaa tcatcgtcat
      421 ccgcgcaaga ccggccggcg cacgcgcaag ttcaattcct gcggtcgcaa tttcgactct
      481 acggttggtg gacggccgca atcaaggctg ccggcgaggg ttgccgccac ggcctgcgcc
      541 tcagcccgcc ggccctgaaa acgcgccgcg ctcgagcagc cacgacgcgg cgaggccgaa
      601 caccaggccc cagaacgggg cgccgacgtt gagaatcggc acgtcggcca cggtgacgag
      661 gaaggtcacg agcgcgccga gcgcgaactt gccgctgaac gagatgctga acgcggtctg
      721 cagcacccgc agcatggcga gccccgcgag cgcggcgatg aatgccttgg gcgccgagag
      781 catcagccgc gtgaacacgg gcgacagcag cccgaagccg agcgcgagca cgccgaccat
      841 gatgcccgcc gtgtagtggc ggtgcttctc gcccgaggcc gtaatgatcg cgttgaccgg
      901 acccgtgagg caggtcgaga cggtgccgac catgccgctc acgagggccc ccacgccgca
      961 cgcggcggtg atcgcgttga tcggcggctg gtgctcggcg gcgcgcagca ccgcgatgcc
     1021 ctggccgttc tggacgacca gcacggtgat cgcgatcggc accaccagct cgagcatcgc
     1081 ctgccaggag aatgccggca ggtacaggtt cgggcgcacg atcgcgaatg cttccgcgcc
     1141 acgcgcctcg aactggccga gcaccgcgat catgaatatg ccgaccacca gcgcgccgat
     1201 caccggcggc agcgcccggg acaggcgcgg tgaggccgaa agggcgagaa acgcgaccgt
     1261 catcggcgcc gcgatccaga acgcatcgcg gatcgcgaac acgagatcga gcccgaagcg
     1321 caggaacacg ccggcgacca tgcccatgac gatcggcatc ggtaccgcgt ccatgaagcg
     1381 cttgacccat ccggagaggc cgagaacgag catgagcacg ccgcaagcca ggtaggcgcc
     1441 gacgatctcg cgaaagccca ggtggccgag cgccgggccg acgagcaccg cgccggggat
     1501 cgaccagaag aatacgagcg gttgccggta gaaccagcag aacgcgatgc tgatgaggcc
     1561 gttgacgaag aacgcgccga agagccacga ggcgaggtcg gactcggaca ggccgccgcg
     1621 catgccgacg gcgagcagga tcgcgaccgg gcccgaggcc gcgaagatga aggcgacgag
     1681 cgcgttggcg gcgtacaggg cgccgaagtc ggcgagcgtc tgtcggagcg agggcggcgc
     1741 aacggccggc ttctcgaggg gcatcggcgg aaaacttcgc gtgctgggaa gggccgatca
     1801 gccctcgtcc cagatcctgt ccgtgagcgg caggtcgcgg atctcggcgg cgccgagcgg
     1861 ccggtccggc gcgatgccga tcgactcgag cagcgccgcg agctggcgca catgctgcgc
     1921 cgaatgccac accgtgcgct cgagcatctc gtgccggctg gtgccgccga agtaggtctc
     1981 gacgcgctgg gtgaagtccg cgccggccgc gcgcgcccac cagcgcgcca agcgctcgcg
     2041 caccgcctcg ccgaacgccg cgatctgcgc gcccgtcagc atgtcctcgg gcggcggcgc
     2101 gaccatcacc tcgtactcga gccgcgtgcc cttttcctcc atctcgagga acgcgacggg
     2161 gatctggaac gcgtggtgcg cgagcacgcg ccaggagcgc ggccggttcg gcagcacgtt
     2221 gccgagcgcc gcatccggaa actggcgcac caggcgcgcc gccgtctcga gcacgacatc
     2281 cgcgcgtgcg gcaagcgcgc ccggcgcgag caccggaccg cccgcttcgg ccaggccgag
     2341 gaaggcgatc acttccgcga tgacctgcgc gtagacgaag cgcccgtcgc gcgccaccac
     2401 cggcaccgag cgcgcgccga gcgcgcgcag cgccgcctgg ccctcggcgt cctcgaggac
     2461 attgaccgac tggaactcga tcccacggac cgagagatac tccttgagcc ggaggcagct
     2521 ggttcagtgc ggctgccaga agacctggat cggtgcgctc atcgcgatgt ccgttgggcg
     2581 gaagcccaag catacctcgc ggccgcgcac gcgtcatggc cgcgccatgc tagattgccg
     2641 ttcgtcacgt catcggcgca tcttcgtttg aaccgcaccg acccgcactt cgccacgccg
     2701 ctgcgtattc cgggcgatcg gtttctgcat gcgcccggcc cgacgcacat tcccgcggag
     2761 gtgctggccg ccatgcaccg ccagcccatg gaccatggcg acccgcgcct cgaccagatc
     2821 atcgccgtat gcgaagccgg cctgcggcgg ctgctgcgca ccgagaaggc cgacctcttc
     2881 atgtacgccg cgaacggcca cggcgtctgg gaagcgacca cggacaacct gcttgatctg
     2941 ggcgacgccg cactgatccc cggcagcggc cacttttccg agcaatgggc gctgcaggcc
     3001 gaagcgaccg gccgccgcgc gctgcgcacg ccctacaagg agggctaccc gatcgacccg
     3061 aacgacatcg agcgggcgct gcgcgcggac aagaagggcg agatcgcagc ggtcttcgtc
     3121 gtgcacacgg acaccgcgag cggcatcacc aacgatctgc aagcgatccg cgccgcgatc
     3181 gacgctgccg gtcacccggc gcttctcgtc gccgacgtcg tcgcctcgct cgccgccgcg
     3241 ccgttcgcga tggacgcgct cggcgtgaac gtcgcggtcg gtgcatcgca gaaaggcctg
     3301 atgtcgccgc ccggcttggg ctttatcgcc gtggacgcgc gcgcggcgca ggtcgccgcg
     3361 aagaacccgg cgccgcgcca ctactgggac tggaagcggc gcaaagggga ctacagctac
     3421 acgaagtttt gcggcacgcc gccgcagaac cttctctacg gcatcgaggc cgcgctcgcg
     3481 ctgctcgatc gcgaaggcat ggacgcggta cttgcccgcc acgcgctact cgcgcgcgca
     3541 gtgcagactg cggtcgagtg ctgggcccag gagggcgcgc tgttcctgca ttgccgcgtc
     3601 ccggcagcgc gctcggtgtc ggtcaccacg atcgaagcgc gcccgggtct cgatgtcgag
     3661 gcgctgcgca gcgttgcgcg ggaacgcttc caggtcgcgt tcgcgggcgg cctcgggcct
     3721 ctgcagggcc gcgcttttcg catcgggcat cttggcgacg tgaacgcggc gatgatcctc
     3781 ggctgccttg ccggggtgga ggccgcgatg cgggtgcagg ggaccccgtt cggtcgcgac
     3841 ggggtggcga aagccgtgga gtgcctcgcg gcaagctgac ctgcagtcac ggcgccagcc
     3901 gcaagtcgcg ctgcgcgagc gccagcacgg caccgaccgc gagcaacgcg gcggaggccg
     3961 ccagcccgac ctgcagcgag cccgaacggt cggcgagcca gccgatcgcc acgggcccgg
     4021 cgatctgccc ggcggcgaac acgacggtga aggtggcgat ggccgaaccc caggcggggc
     4081 tgggcagcgc gcgcttgatg aagctgccga ccgacgacgg cgcgctgaac atggccaggc
     4141 cgaacagcgc ggcggacagc agcatggccg caggatgcgt ggagacgagc ggcagcgcgg
     4201 ctcccgcggt gagcacggcc atcactgcgg cgagcgggcg gccgccgggc cagtgctcgg
     4261 cgggcttgcg ccacaccagc ggcgcgagca gggtcgccag ccccagcacg aaccacaccg
     4321 cgattaccga cgcggtgctc gcgccgttga cgcgcatcca cgcgatgacg aaggtcatgt
     4381 agccgatgta gcccagcgcg aacagggcgt aggaggcgag ctcggcagca aacggccgca
     4441 cttcccagcg cgcctcgccc ggcattctcc ctggctcggc gatgcgcaat gcggcccagg
     4501 ccgcggcccc ggtcagcaac aggctcgcgt agcccatgcc gcgccaggcg agcggccagg
     4561 acgcgctgcc gcgcgcctcc agcagcagcg gcagcgcgat gcccgagagc atcaggccga
     4621 ggccgccgcc ggcgaagtag atcgcgatgg tcgtcgtggc gagctgcggg cgcttcggaa
     4681 agatgttgcc cgagagtgcg ccgccgcaga tgaagaccgc ggcgccaccc acgcccgcca
     4741 gcgcgcgcac cgccatcagc gcgtcgaagt cgcgcacgaa gccggtagcg gcgatggcga
     4801 cggacgtgat caccatgccc cagccataca gcaggcggtt gcccaggcgc gccaccagcg
     4861 cgcgggtcag cagcgcgccg acgaggtagc ccagcgcgtt gacggtgttg atcgagccgg
     4921 cctgcgagta gctccactcg agttcgctgc gcatcgccgg caggatgagc gcgtaggcga
     4981 agcgcgagaa ggagttcgag accgccgggc cgaacgagag caggaacgcg atcagcagcc
     5041 cgcgcgccag tgagatgtcc cggggatcgg cgcccacggt gctagagatg cgcgttggcc
     5101 gccgccgagt ggtgccacag cgcgctgcgc aagcggtagc cgatgacgag cgtcgccgcc
     5161 agcccgatcc cgccgtagat cagcgtcgtc gcgccgtagc cgatccccgc gatgatcggc
     5221 cccgcggcaa gcagccccaa gggcagcccc cagatcgcga gcatgcggat ccccatcacc
     5281 cgaccgcgca tctcctcgct cgaggcgcgc agcatcaccg cggcgagcgg cgtcatgcag
     5341 aagctctgca ccaggcccga caggaacagc agcagcaggc cgacgctgat gaagggaatc
     5401 tgtccgaaga gcacgaccgc cgtgaaccac gcggcgccgc tccacatcat gatgcgcccc
     5461 gcgcgcagcg gcagccgcgt cacgctcagc agcaccgagc cggccagggc gccggtcgca
     5521 aacgaggcgg ccaggtaccc gagacccgcc tggcctatgc cgtacacgtc cttcgcgacg
     5581 tacggcagca ggccgaggaa gaagggatac gcgagcaggt tgacgagaaa tgccatcccc
     5641 agcgcgccca gcaggtcagg tttgccccgg atatagccga tcgcctgctg caacccggca
     5701 agcaccgcag cagtctcggt cttgccgcgc accggcgttc ccgcgacgcc cagggacagc
     5761 aggaatgcgg caacgtaaat cgccgtcacc accacgtacg ccggccccat gccgatcagc
     5821 gccacgccgc cggtgccggc gagcgcgccg gcgatgcgcg cggtgtcgga cgtggtgcgc
     5881 gagatgccga gcgcgcccat caggatctcg gggcgcatgg tctgcccgac gaggacgttc
     5941 ctcatcgcca tgtcggaagg gcgcatcagg ccggcgagcg cggcgatgac gaagacgtgc
     6001 caggcggcca gcgctccggt cagcgtcagc agcgccagca ccgccgccag cagcgcgtag
     6061 gccccgcgcg tgatgcaaag cagcgcgcgc aggccgatgc ggtcgccggc gacgccgaag
     6121 aagggcgaaa acagcgaccc gagccacgcc agcgcggcga ccagcacgag cttctcgacc
     6181 gagcccgtgg tgctgagcac gaaccagccg aggatcagtg cctccatctc gaaggcccag
     6241 gaggtagcga ggtccgccgg ccactggaag cggaagctgc gcacgccgaa gggcgcgagc
     6301 gttggcgcgc gcggcatcag tgtgcgcccg accggctact tcgcgcgcca gccgccgtcg
     6361 acgacgagcg cgtggccgtt cacgtagtgc gcggcgtccg aggcgaggaa gctcaccgcg
     6421 tcggcgacgt cctcgggcgt gccgcgtacg ccgagcggca cgagtgcctt caccatctcg
     6481 tcgttcaccg ccacgccgcg cccgctcatg tcgggcacgc ccgggccgag gatcgcctgc
     6541 gagtgcgcgc gcaggcccgt cgggatcgcg cccgggcaga cgcagttggc gcggatgccg
     6601 cgcgcaccgt actgcaccgc catctggcgc gtgaggccga cgacgccgtg cttggccgcg
     6661 atgtaggcgg cgccgccgcc ggtgccgttg aggccggcga ccgaggacat gttgatgatg
     6721 cgcccgcgct ttcgcggcag catctcctgc aatgcgcgtt tggagccgag gaacacgccc
     6781 gtcaggtcga tctcgatcac cctgcgccag agcgcttcgt ccatctcgtc gacgtcgaaa
     6841 tagccatcga ggatgcccgc gttgttgacc atcacttcga gcgtgccgag ttccttgatt
     6901 gccgccgaaa cggccttgtc cagctccgcg gcgcggctga cgtcggcgcg gtacgcggcg
     6961 gccttgccgc cgatctggcg cgcggtctgc ttcgccgccg cctcgtcgag gtcgatcgcc
     7021 gccacggcga agccgtcggc ggcgagcttc ttcgcgatcg cctgcccgag gcccgagccc
     7081 gcccccgtga ccaccgcgag gccgctcatg ccggcggctc cgcgcacacc tgctcgtagc
     7141 ggtagcggcg cttcagttgg ccgttgaact cgaacacgtc gagggtcttc tcgtaggccg
     7201 cgcgcgtgat ctcgacgtgc cgcgtccagc agcccagctt ctgataggtg gcgatgcaat
     7261 cggcgagcac cgcctcgtcg atcttgggaa agaagggctt ctccgccttc gcgatctgcg
     7321 cggccggcgc gtcgttcatg taggcccggg tcttgcggta ggcgcgcatg aacgccttgg
     7381 ccatgtcgct tttcagccac tcgcgcgtcg ccgcgaggct ggaaaacccg cacggcccga
     7441 tctgtgggcc gacctgcgcc acgacgtggc cgacgccgtc agcctgcaat tgctgcggga
     7501 aggggccctg ctggtgcaca tactgcccct ggccttcgcg gaacgccttg tcgatgtcgg
     7561 cgggaccgcc gggcgtgacg agcttgatct tcgagtagtc gatgttcgcc ttgtggcagg
     7621 cgtacttgaa catcgcggcc ggctggccgc ccttgaaggt gacgagctcg gcgccctcga
     7681 gccttgccca gctgaacgcg gcgtcgggct tgcgcgccgt gaggaaaaac ccgtccatct
     7741 cgttcacctg ggcgaaatgc acggccgacg gcgtctcgcc cttgtcgagg gtgacgaagc
     7801 cctgcgacag cgccgattgc acgacgtgcg cagagccgtc cttcagggac tggagcgccg
     7861 aaacgccggg cggcgacacc gaccactccg ggtccaggcc ctcggccctg agaaagccgc
     7921 ccgacatcgc cgagatcagc ggcgaataaa acgcggagaa cagcgtgaac tggatgcgga
     7981 ttttttccaa cagctactcc tccctgacga aatcgtactt gccgacgccc tgaatcagca
     8041 agaaacggca ccgcgatttc gcagagttgg acacctggtg cacggtgccc ggcgtgacgc
     8101 tcgcgctttg ccccgcgccc aggtcgtggc gcgccaccgg attgcgcgac tcgaccacca
     8161 ccgtgccttc gaggccgtag tagtagtcgg cgacttggct gtgccggtgc cacggaatgg
     8221 tgtcacccgg gtcgagcgtg tactcgcgta ccagcatgtc gtcgctcttc gcgacgacat
     8281 ggatgttgcg caccttgtag ggctgcggct tcacgcgggt ctcccggaat cagtctgggc
     8341 gcaggtccca gtggggcgag tcgtggctga cttcgagcag gcgctgcacg aacgccggaa
     8401 cggattcctg gaccgtggat acgtagtctg gcctcaggcg atcgcgatgc cggggcgtgc
     8461 tcatgacgcg catggcatcg gcgagcgtga ggattcccgc ctccgtgccc ggcttgaacc
     8521 cggaccactt ctttacgtgc tctttcgacc gaaagaggtt catgcggctt caggcaaagg
     8581 gcaggttggc gaaccacttc ttgatcggca gctcgacgta gaagtggatg tcgcccggct
     8641 ccgcgcgctc gatctcgccg tcgacgattt cgacccggat cggctcgccg caatcgaggc
     8701 agggggcgct caccgtcacc cgcttgcccg ggaagagcca ggtcatcgcg agcgattcca
     8761 gcccgcactg cgcgaaccac ttctgctcgc cgtccaccga tatccggtac tgcgtcggca
     8821 ggttgttgaa cggggcgaac gaggcgatga ggtcggtgtc cgggaacagc cagttcggca
     8881 ggcgctcggc catcagctcg cgcagccggc gcctgccttg ctcggcggga atgccgaact
     8941 tcgctgcgat ctcggtgaaa tgcggcgcct ggccgcgcgc cacgagcgtc gagaggatgt
     9001 aatggtaggt ctggtcgagc tgcgccgcgg ccatccccga agtggacgcc cggcgcaatc
     9061 cagagtcaag taccgcgccc gggttgccgg gcgagtgtca gcgcgcggcc agcggtttgc
     9121 tgatcaggcc gccgtcggga cggggcgcgc gcttgcgcag gcgcgccgag aggaagatcc
     9181 cgatgagtgc cgcggccacc gcgatcgccg ccgacccgat ccacgcgccg accgccaccg
     9241 cggccaggcc gccgtgggtg agccgatagc ccacggcgac ggcgccgcag gtggcgagca
     9301 ggatcagcag cggggaaacc acgcacagaa tcgcgtcgtc cgaggtgatc cacaggtcag
     9361 ggcggtggcg gcacgcctcc cgcaccagcc cggcgacgag cgcgacggcg cacagcgccg
     9421 ccacggccga catcatgtac atcagctcag aatgcccttg agcaccagcc cgccgccgat
     9481 gacgaacgcg gtgacgatgg cgagcgccag catcgtggag cgggcctcgg ttcgggtcca
     9541 gaagctcgcg ggccggccct ccttcgggct gacggtgtac aaggtgtagc cggagccgaa
     9601 taggcaagcc acgccggaag caacgagtgc tgcactggtc aagaccatga tgaaacctcc
     9661 tggcgcgatt gtcggcggca ggggcattaa cgttccatac gcgggcgggc gcagcggcat
     9721 aactgccgcg taaacgctca gggcggcagc tggtccggcg tgcgcagccg gtagcccacg
     9781 ccaagttccg tctgcaggta gaccgggcgc gccgggtcga cctcgatgcg gctgcgcagc
     9841 ttcctcacga acacgcgcag gtactggacg tcctcacggt gcgccgcgcc ccagatgtca
     9901 ttcagcagct gccggtgggt gaccaccttg ccggcgttgg tcaccaggat ctgcagcagc
     9961 tggtactgct tgggcgagag ccgaaccggc gcgccgctca cccgcaccac gcggcgcacg
    10021 aggtccactt cgagggcgcc aacgcgaaat acgggcgccg gcgccgcggc ctcgacacgg
    10081 cggcgcagcg ccgccttgat gcgggcgagc agctcgggca tgccgaaagg cttgaccacg
    10141 tagtcgtcgg cgccgagctc gaaggcgcgc accttttccg cctcgcggct gcgcaccgag
    10201 agcacgatca ccgggacgga cgaccactcg cgcaagcgcg cgagcacgtc gaagccgtcg
    10261 ccgtccggca agcccaggtc gagcacgacc agatcgggcg gcgccgcggc cgccgcgctg
    10321 agcgccgcgg cggccgagcc cgcctcgtgc accgcatagc catgcccttc gaggccgaag
    10381 cgcagaaact tgcggatctg cggctcgtcg tcgacgacga ggacccgcgg ccgggactcg
    10441 ctcatgccgg gagccggatc tgcacccggg tgccgcgccc gggccccgcg ctggcgaggg
    10501 tcacgctgcc gccgttggcc tcgacgaaag cgcggcagat ggcgaggccg aggccgctgc
    10561 ccgcggcggc gccgccttcc tgcgggctgc cgcggtagaa cgcctcgaaa acgcgatcga
    10621 gctcgtcggc gcggatgccg cggccggcgt ccgcgatctc cagcagcacg ccgccgttgg
    10681 ccggccgcgc ggagatgctc accgtcgagc ccgcgggcga gaacttggcc gcgttgtcga
    10741 ggacgttcgc cagcgcatgc tccatcagaa atagatcgag ccgcagcatc ggcaggcccg
    10801 ccggcacgtc gacctgcagc gtgtgcccgg ccagcgcctt gtccttgcgc cgcagcgcgc
    10861 cgttgatgat atcggagagc tcgaccagct ccaggcgcgg cgcgatctgg cccgcgcgca
    10921 cgcgcgtgag atccagcacg ttgccgatgt ggctggcgag gcgctcggcc tcctcgcgga
    10981 tcgtcgccgc cagctcgcgc cgcacccgcg gctcgccggc gtcgcccagg gtctccagca
    11041 cgctcgccga gccgaggatc gccgcgagcg gcgtctgcag gtcatgcgac atcgagttga
    11101 tgagcgcctc gcgcagcgcc tcgccctgcg ccttgaggcg cgcgtcggcg atctcgcgcg
    11161 cctgccggcg cacgccgtcg gcgagccggc tggtcagcac cgcgacgatg atgaataccg
    11221 cgaggtcggc aaagtcctgc gggtcggtaa cgtggaagct gtagaccggc gagtagaaca
    11281 ggtagctgcc ggcggccacg ctgaggaaag cggcgaagag cgatggcccg agtccccaga
    11341 gcgccgcact ggtaatcacc gcggcgacga acagcacgga cacgtgcggc aacggcacga
    11401 gccgcgtgag cgcgtgcgcg gcgaggccta cccccgccac ggccagcgcg ctcttcaggt
    11461 aggcaagcag ggccaggcgg ccgcgcacgc gggcgctact cgcgcacgcg cgcgagcttc
    11521 tttacgtccg gcacgcgcct gagcgcgcgc atcacgcgcg ccaggtggcc gcggttcgac
    11581 acctcgagca cgagatgcat gttggtgaag aacgcccggt cgtcctccat gctgatcgag
    11641 tcgatgttgg cgccggcctc ggcgatctcc gaggcgacct tggcgagcac gccgcgctgg
    11701 ttcgcgacca tgactttgat cgcggcctgg tacaggcgcg tggtcgcggg cgcccactcg
    11761 acgtcgatcc actgctccgg ctcgttcttc cgcgagcgct cgatgacgcg gcaatcggcg
    11821 ttgtgcacca ccagcccctg gcccttcttc atggagccga cgatgccgtc gccggggatc
    11881 gggcggcagc acgaggcgag ctgcaccgcc atgccctcgg tgccgcggat gaccacgctc
    11941 accgccttgg agacgggctt cgcgtcggac tcggcgcgca tcaccagccg ccgcgcgacc
    12001 acggcgggca gttgcttgcc caggccgatg tcggcgagca gctcgcgctt ggtgcgcccc
    12061 acggtctcgc ggatgacgcg ctcccagctg gcatcgtcga tgtcggtgat cgccacagcg
    12121 tagccgttca gcgcctgctc cagcagccgc tcgccgagcg ccgccgactc ctcgtactgc
    12181 atggtcttca ggaagtgccg gatgttggcg cgcgccttgc cggtgcgcac gtactgcagc
    12241 caggccggat tcggcttggc gtggctggcc gtgatgatct ccacgcgctc gccgttgcgc
    12301 agctcggtcc tgagcggcac cagctcgccg ttcaccttgg cggcgacgca acggttgccg
    12361 atgtcggtgt gcacctggta ggcgaagtcc acggcggtgg cgccgcgcgg cagcgacagg
    12421 atcttgcccg ccggcgtgaa cacgtacacc tcgtcgggga acaggtccac cttcacggtc
    12481 tcgaggaact cgtgcgggtc gcccgactgg ttctggatct cgagcagcga ctgcagccac
    12541 tgatgggtct tcttctgcag atccgacagc gacaccgcgt cgtccttgta catccagtgc
    12601 gaggcgaccc cggcttcggc gaggtggtgc atctgctcgg tgcggatctg cacctccagc
    12661 ggcacgccat aggggccgat cagcgccgtg tgcagcgact ggtagccgtt cgccttggga
    12721 atggcgatgt agtccttgaa cttgccgggc accggcttgt agagcgcgtg cagcgtgccg
    12781 agcgccaggt agcaggtggc gacgtccttt accaccacgc gcaggccgta gatgtcgtgc
    12841 acctcggaaa acgaaaggtg cttctccatc atcttgcggt agatggagta gatgtgcttc
    12901 tcgcgcccgc tcactgcggc ggcgatgccc gcgtcggtga ggcgcttgtt caccgccgat
    12961 tcgatcttgc cgaccacctc gcgccggttg ccgcgcgccg ccttggtcgc cttgacgagc
    13021 gtgcggtagc gcagggggta caggtgcgcg aacgcgagct cctgcagctc gtggtacagg
    13081 gcgttgaggc cgagccggtt ggcgatcggc gcgtagatct ccatcgtctc gcgcgccacg
    13141 cggcggcgct tcgcgggggg caccgcgtcg agcgtgcgca tgttgtgcag ccggtcggcg
    13201 agcttgatga ggatgatgcg cacgtcgcgc gccatcgcca gcagcatctt cctgaagttc
    13261 tcggcctgcg cgtcctcggc ggactggaac tcgatcttgt cgagcttgga caggccgtct
    13321 accagctcgg ccaccggcgt gccgaaggtg tcggagatct cggccttggt gaccgcggtg
    13381 tcctccatca cgtcgtgcag cagcgccgcc atcagcgcct ggccgtcgag gcgccagccg
    13441 gcgaggatct cggccaccgc cagcgggtgc gaaatgtacg ggtcgcccga ctggcgcttc
    13501 tggcccgcgt gcgcggcctc cgagaagcgg tacgcgtccg ccaggcgcgc gatgtccgcc
    13561 ttcttcaggt agccgaagcg gctggcgtcg aggctttgta cggcggcggc catccgggga
    13621 gattacccca gggccgaccc ccttcggggg ctggtcccgg ggctcagggc gccgggggcg
    13681 tgggcacgat cgggttgcgc ttcaggactt cggtgccgac cttgccggtg gcgatctcgc
    13741 gcagggcgat caccgtgacc ttgtcgcggt tgggctccac gagcggcgag gcgccggcgc
    13801 cgagctggcg cgcgcgatag gtcgcggcga gcgtgagctg gaagcggttg gggattctct
    13861 gcaggcagtc gtctacggtg atgcgggcca tggtcagggt tccggtctga agacttcggg
    13921 gtgccgcgcc agctgccggg caacggccag gcgttctgcg cgcacgaccg cccgcagatc
    13981 gagcagggcg tcgtcgaagt tattgttaat tatagcgtat ttgaactcga cggcgtgcgt
    14041 catttcgtcc gccgcgctgg cgaggcggcg ctggatcacc gcgtcggcgt cctgggcgcg
    14101 ggcgcgcagc cgccgctcga gttcgcccat cgacggcggc aggatgaaaa cgccgatcgc
    14161 gtccgagtgc agccgccgca cctgcatggc gccctgccag tcgatttcga gcatcaggtc
    14221 gagccccgag gcgcgagctt tctcgatcac cttttgcgag gtgccgtagc ggtggccgtg
    14281 cacctgcgcg ctctcgagga actcgccgcg ctcgagcatc gcctcgaact cgcgcagccc
    14341 gacgaagtgg tactcgcgtc cgtcggcttc gcccgggcgc ggcgggcgcg tggtgtagga
    14401 caccgacagc gcgatgccgc gctcggccgc gagcagcccc ttgatcagcg aggacttgcc
    14461 ggcgcccgag ggcgcggtga ccacgaagag caacccgttc attcgatgtt ctgcacctgc
    14521 tcgcgcacct gctcgatgag cagcttcagc tccagcgcgc aatcggcgat ctcgcgcgtg
    14581 gcggccttcg aggcgagggt gttggcttcg cggttgagct cctgcgcgag aaagtcgagc
    14641 cgcttgccgg cagcgccgcc cgcggccagc acgcgctccg cctcggtgat gtgcgtgccg
    14701 aggcgctgca gctcctcctc gacgtcggtc ttcaccgcga acaccgcgag ctcggcgcgc
    14761 aggcgctcgt cgtcggcggc gccgagcgcc tcgcgcagcc gctcggcgag ccgcgcctgg
    14821 taggccgcgt tcgcctccgg caccagcggt gcgatcgggg cgaggcgctt gcgcatttcg
    14881 gcgacgcgct cgccgaggat cgacgccagc ttcgcgccct cgc
//
LOCUS       DBNHKE010000201        27391 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_988346, whole genome shotgun sequence.
ACCESSION   DBNHKE010000201 DBNHKE010000000
VERSION     DBNHKE010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 27391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27391
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_988346"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="AC16O9_13805"
     CDS             complement(<1..707)
                     /locus_tag="AC16O9_13805"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR6517724.1"
                     /translation="MSGKAPQKTLLLVDGSSYLYRAFHALPELQSPRGEPTGAIRGVL
                     SMLRKLAEDYKADALACVFDARGPTFREAVYPQYKANRPSMPEPLAAQIEPLKEAIAA
                     LGWPVLAVEGVEADDVIGTLAAEATQRGWRTVVSTGDKDLTQLVDEHVTLVNTMSNET
                     LDVEGVKRKFGVAPGQIVDYLTLTGDAVDNVPGVDKVGPKTAAKWIQQYGSLDEVMRH
                     AGEIGGAVGANLQRSLD"
     gene            709..1425
                     /locus_tag="AC16O9_13810"
     CDS             709..1425
                     /locus_tag="AC16O9_13810"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR6517725.1"
                     /translation="MSNDKLPRPASRAPEISAREAWRVFGIMAEFVESTERLADIRPA
                     VSMFGSARVAPGSPYYRLAEEVSRLLSDAGFAVISGGGPGVMEAANKGAHAGISPAIG
                     LNIDLPREQSGNRFQDISLRFKHFFARKVMFVKFATAFIILPGGYGTLDELFEALTLV
                     QTGKTRKMPIILMHAPYWRGLLAWIRERLVAEVMIDPGDADLLQVIDEPRAVVEAIFS
                     YYEKRGFEPSPAEREVLLNL"
     gene            1500..2456
                     /locus_tag="AC16O9_13815"
     CDS             1500..2456
                     /locus_tag="AC16O9_13815"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR6517726.1"
                     /translation="MSVYTAVSQPELAAWLRNYSVGALEALEPIETGIENTNYFVTTA
                     QGRYVLTLFERLPAAELPFYLNLMAHLARHGIPCPAPVAGLDDAYLGELNGKPAALVT
                     RLPGRSLERPGPDECAALGALVARMHLAARSYGAYLDNPRGPRWWRLAARDLEPFVDA
                     GERRLLEAELAFQARQRFPDLPRGPVHADLFRDNALFEGPRLSGVFDFYFAGVDCLLY
                     DVAVCANDWCLADPARDRRLDPARLQAFLGAYAALRPFSAEERDAWPAMLRAAALRFW
                     LSRLHDLHLPRPGELVHAHDPAHFRDILERRAAEAPPWLAAP"
     gene            2453..3226
                     /locus_tag="AC16O9_13820"
     CDS             2453..3226
                     /locus_tag="AC16O9_13820"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR6517727.1"
                     /translation="MNARLVSPMQGARWLAAGWRMFRAAPLGWLVLVFAYLMATMLLS
                     AFPLIGPAAVSVLMPAFSVGFMAASRAAARRQAVELPMLFAGFRERLPQQLVLGCVYA
                     AGFAAALAGSALLDDGALLGVLFSPQRVQAEGGPGDALLLGVLASALIYLPTMMMLWF
                     APVLVAWHAVHPAKALFYSLVAFWMNRTAFIAYALALALVLFLSMGSVVLLAALMPGE
                     APAFNPRSLVFPMALVILPTLFASYYASYQDVFGAAGEP"
     gene            3226..6966
                     /locus_tag="AC16O9_13825"
     CDS             3226..6966
                     /locus_tag="AC16O9_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HIR6517728.1"
                     /translation="MQPRLREIPYNYTSFSDREIVLRLLGAEGWRLLAELRAERRTGR
                     SARMLYEALGEMWVIRRNPYLEDGLIADRRARRQLLEGMRRRLAEIEARRAGNEKVGE
                     LLALAGAALERFAASFEETRALRARVLKRLRRVTRRDNIAFDPYARVAHVTDATDWRV
                     EYPFVVVSPDTEEEVLMLVRALGALGLTIIPRGGGTGYTGGAVPLTPRAAVINTEKLD
                     TLGAVEWLELPGAGRKTPTVRAGAGVVTRRVMDAAEEAGLVFAVDPTSAEASCVGGNV
                     AMNAGGKKAVLWGTAVDNLAWWKMVTPQGDWLEIERYQHNLGRLQDTASATFHLRRIS
                     PDGKKLLEERAIELPGSAFRKQRLGKDVTDKFLGGLPGVQKEGCDGIIVAARFILHRM
                     PAHTRTVCLEFFAPAAEAVPAIVEIRDHVARLAGVQLAGLEHLDERYVKAVGYGTKSR
                     RGQRPRMVLIGDLVGANVDEVASAASSVVRIANARGGEGFVAVSAEARKRFWLDRART
                     AAIARHTNAFKINEDVVIPLEHLGAYSDGVERINIECSIDNKLALLDALEEYFQGALP
                     LVQGEEALPRPELLGDRAQQALDLLAAVRTRWQRLRNGIDFEFAKLQARTERLSWKTE
                     VRAELARIFAGPPYAKILEEADALHKRVRRGRVFVALHMHAGDGNVHSNIPVNSDDYA
                     MLQRATQVVARLMALAKSLGGVVSGEHGIGITKLEYLEPAELEAFRAYKEAVDPEGRF
                     NAGKLLAGGGLENAYTPSFSLIGAESLILEETDLGAVTRSVKDCLRCGKCKPACATHV
                     PRANLLYSPRDKILAASLLIEAILYEEQTRRGVARVHYDAFADVAEHCTVCHKCASPC
                     PVDIDFGDVSIAMRNLLRRAGKARFNPGTRASMLLLTATDPRAVRAARLALVGWGFRL
                     QRAAHRLLRLLWRGQTARPPATTGQPPLRAQVVHFVNKALPGALPKKTARALLDIEDA
                     KIVPIVRDPAKVGEDSDAVFYFPGCGSERLFSQVSLAAQAMLYEVGAQCVLPPGYLCC
                     GYPQSAAGEQDKGERIITANRVLFHRLATTLNYLDIRTVIVSCGTCLDQLEKYEFERI
                     FPGSRLIDIHEYLLEKGVKLEGVEGQRYLYHDPCHTPMKLRAPLETVRALTGGAVQPA
                     ARCCGESGTLATTRPDISTQVRYRKEEELKRDVAALPGDGPVKVLTSCPSCLQGLARF
                     DADAGTQADYIVVEMARRLLGENWLPEFVGKANTGGIERVLL"
     gene            6978..8591
                     /locus_tag="AC16O9_13830"
     CDS             6978..8591
                     /locus_tag="AC16O9_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517729.1"
                     /translation="MSPPSALPPPAIDPAAQPEFSDAASAKAWLEHVPLANVSAAQHQ
                     LLVQIEEFNRYAAGPEARFAVMETLREAVNFVQIEQAKRFTNRALPMQAAEATLFEET
                     IELWEQMRLGYLRCLEADAAALRGKRALLAQRALAYSGLRMFHHYRAYRQVPARDWRN
                     LHQTYARAEHLGVTETAVKDYLNRDVHDTSPRIAYLRATLMGMCNPHELTPRQLTFVA
                     YLLERWADKVEISGAPVDEGDLPPLSVDLAGERCAERGTAAGAELRYLDARRLAKSIR
                     NRVALLRKGESPARLALGEDCVQPSCEQLLVFLYRQWCQARPARGVDRDRAQDKVQAC
                     TGLAAIHYYVSGRVFRVTATFKELTARQRDEIATFGRVSTRDEDDYSVAQGFLLEQWQ
                     LEDESAQGLKMLRRAGSPGQRMAAGQLVGVRHADAKSFQIGQVRWLLATEAGDLVCGV
                     KLLPGLPLAGAVRGTGVNAADEKYVQAVALAAVPALKAPPTLVLAPGWFRPKRVIEAA
                     IPKPMRARLLEVLERGNDFERVAYEVLPD"
     gene            complement(8588..9883)
                     /gene="phoR"
                     /locus_tag="AC16O9_13835"
     CDS             complement(8588..9883)
                     /gene="phoR"
                     /locus_tag="AC16O9_13835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HIR6517730.1"
                     /translation="MNPAWASALAVAVAAAAAGLLWGWGGVAAVLALALLFHTVQLAA
                     FLRWLSRPSRDALPPGRGLWEDAFATLHRHLKDRDDERERLRAALARFRDAGRALPDG
                     VVILDREYHIEWANPTAARHFSIDARRDLGRPVANFMRHPAFIAWLEAGRFDEPLVLR
                     GVRGETVLLVRLIEFGDEQKLLISRDITAQEKLETMRRDFVANVSHELKTPITVLAGF
                     VETLGEAEIEMSPAQRRRYLGLMAEQARRMQQLIEDLLTLSALESSPAPADEQAIDMR
                     ALVERLVEEARALSAGRHQVVSRIEHDALLTGSARELHSAFANLVSNAVNYTPPGGTI
                     TLSWRLEGKRGIFAVQDTGIGVETRHIPRLTERFYRVDQGRSRDSGGTGLGLAIVKHV
                     LTRHQASLEVESAFGKGSTFRAEFPADRLAPAGPARAVA"
     gene            complement(9880..10572)
                     /gene="phoB"
                     /locus_tag="AC16O9_13840"
     CDS             complement(9880..10572)
                     /gene="phoB"
                     /locus_tag="AC16O9_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HIR6517731.1"
                     /translation="MPANILLVEDEPAIQELIAVNLEHAGHQVIRAKDAEGALGIVRP
                     ALPDRLLIDGMLPGMSGVALARQMRREDRTRAIPIILLTARGAEADKVAGLEAGADDY
                     VTKPFSPRELLARIKAVLRRRAPQMTDDPVELAGLRLEPETHRVFGASRALELGPTEF
                     RLLHFLMTHAERVHSRAQLLDQVWGDHVFLEERTVDVHIRRLRKSLEPSGHDRLIETV
                     RGAGYRLTALSA"
     gene            10640..10987
                     /locus_tag="AC16O9_13845"
     CDS             10640..10987
                     /locus_tag="AC16O9_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR6517732.1"
                     /translation="MPTPTEIRLHQKSRLLEVVFDDGRSFRLPYEYLRVFSPSAEVRG
                     HGPGQEVLQVGKREVEIRQVEPVGSYAIQPTFSDGHATGIYSWDYLYQLGVEQDARWA
                     AYLKQIEEAGASR"
     gene            11007..11741
                     /locus_tag="AC16O9_13850"
     CDS             11007..11741
                     /locus_tag="AC16O9_13850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6517733.1"
                     /translation="MFDTDFGYEQVHESEKRARVGEVFDQVAPRYDLMNDLMSLGVHR
                     LWKAFAVNVARVRAGDRVLDIASGSGDLARVFARRAGTTGEVWATDINRRMLERGRDR
                     LLDAGLRLPAVQCDAERLPFPAGYFDCVAVAFGLRNMTHKDAALAEMARVLRPGGRLV
                     VLEFSRVWKPLEKPYALYSFSVLPWLGKQVAGSGDAYRYLAESIRRHPDQDTLAAMME
                     RAGLAAVEYFNLSAGVAAVHRGVKLG"
     gene            11788..12618
                     /locus_tag="AC16O9_13855"
     CDS             11788..12618
                     /locus_tag="AC16O9_13855"
                     /inference="COORDINATES: protein motif:HMM:NF016190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HIR6517734.1"
                     /translation="MSKGWFGITAAVLLGLVIAMPEAEARRLGGARSLGVQRNVTAPP
                     AAVPAKPAQAAPAAPAAGTGSKLAPMLGGLALGGLLGAFFAGNPVLGTLLTALLVGML
                     VFAGIALVRVLRAPRTAPPAFQYAGLGSETVAAPPPSQAAGLEAREPSSPARAHLPAG
                     FDAAGFLRAAKENFIRLQIANDSGELEALRDMTTTGMFEALRNDAGAGQQTDVVTLSA
                     DLLEVTTEGGRHWASVRFSGLVREAPGAEPVSFEEVWNLTKPADGSSGWLLAGIQQMH
                     "
     gene            12668..13234
                     /locus_tag="AC16O9_13860"
     CDS             12668..13234
                     /locus_tag="AC16O9_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiJ"
                     /protein_id="HIR6517735.1"
                     /translation="MATLESPLVLALNRLLEAEGWARERLAPFAGETVELRAPPLPAL
                     RFSIVEGGRLAPGTAASLTLTLRPDSLPALLRGEEHFMRTVGIDGNAQLAREVLHLAR
                     HLRWDAEEDLSRLVGDAAAHRAVMLARDFAAWQLDAGRRLAEAFMEYAQEEGRLLAPR
                     AGYAAFAAEVAQLRDALERLEKRIGRLA"
     gene            13231..14703
                     /gene="ubiB"
                     /locus_tag="AC16O9_13865"
     CDS             13231..14703
                     /gene="ubiB"
                     /locus_tag="AC16O9_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR6517736.1"
                     /translation="MRRLARIVWVALRFRLYRFWPRFARAADPERAVRLRRALETLGP
                     IFVKFGQVLSTRRDLLPLDIADELAKLQDRVPPFDPVLARAEIERGIGKPIDAVFSSF
                     EREAVASASIAQVHLARLHDGAEVAVKVLRPGVERAIARDVALLETAARLVELLWADG
                     RRLKPREVVAEFARHLEEELDLLREAASASQLRRNFEASVLLAVPRVYWDWCSQRVMV
                     MERMHGTPVSQVERLREKGVDIPRLARAGVEIFFTQVFRDGFFHADMHPGNILVTDEG
                     QYVALDFGIMGTLTETDKNYLAQNFLAFFKRDYRRVAQAHLDAGWVPEGTRIDDFEGA
                     IRAVCEPIFARPLKEIYFGKLLLRLFQTSRRFNVEIQPQLVLLQKTLLNIEGLGRTLD
                     PDLDLWQTAKPYLERWMSEQIGWRGLARALAQEAPTWAATLPQLPRLAHRLLAEDRLG
                     ALERALARIGEQNVRRNRLLAAIAALLAAALILIMMAAAC"
     gene            14697..16127
                     /locus_tag="AC16O9_13870"
     CDS             14697..16127
                     /locus_tag="AC16O9_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high affinity choline transporter 1"
                     /protein_id="HIR6517737.1"
                     /translation="MLIAFVALYLVASIAIGLWAARRVHNSRDYVVAGRSLPLYMSFA
                     LVFATWFGAETVLGVSATFLKDGMGGIVADPFGFSFCLVLIALFFARAFYRLNLLTIG
                     DFYRKRYNKPVEVATSVCITLSYLGWTSAQFTALGFVFHALSGGAIPLHWGIALGAGC
                     VLVYTLFGGMFSIAYTDLFQTTIIIVGLIYLAFLLGGMAGGPGAVLAHAAQADKFQFW
                     PKPELKEILAFVAAFLTAALGSIPQQDVFQRVTSAKDADTAVRGTMLGGIVYFFMAFV
                     PIYLAYSALLIDPQMVAPLIEGDANENQLILPNLILGHTPVFAQVMFFGALLSAIMSS
                     ASGALLAPTSLFTENVLRPFLPNMSDRQFLLTLRLVLVIFALAVLVFALESDASIYQM
                     VQNAYKVTLVSAFAPLVLGLFWNRATTAGALLSVVLGLVTWLLMEAVAPEALVPPQLV
                     GLAASFLGMVVGSLASRPAHARAHPG"
     gene            16131..17189
                     /locus_tag="AC16O9_13875"
     CDS             16131..17189
                     /locus_tag="AC16O9_13875"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6517738.1"
                     /translation="MRGSPLARLLLFVYALLVVYASLHPLAGWRDHGLMPFEFLAAPW
                     PRHVVLFDVAADVVGYAALGALAVLAWHPRLRRGRAVLAATALGLVLSIVLESVQTYL
                     PTRVASNVDVLCNTAGALAGALAGLAFAPLSPALRGWRARLFLPGTLSDLGVALLGLW
                     LFMQLNPAMLLFGGGDLRNLIAGPGGAARAPEFFAAIEAITAAANLAALALLASVIVA
                     PGAPVRRLVLLLLTAALAVKTLAFTIILRAQDVFAWLTPGAQRGLAAGLVLAMLAVAL
                     PRVARMALAAVLLTAAAVLVNHAPPNPYLAEILKLWQQGHFLNFNGLTRLVATLWPFA
                     AVAYLIVLAARRRDEGLL"
     gene            17202..17516
                     /locus_tag="AC16O9_13880"
     CDS             17202..17516
                     /locus_tag="AC16O9_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR6517739.1"
                     /translation="MSYYERHVFFCCNRRDASDPRGCCSAKGASDMRDYAKQRVKELK
                     LAGAGKVRINQAGCLDRCEQGPCAVVYPDAVWYTYVDRGDIDEIIEEHLRHGRIVERL
                     RI"
     gene            17516..18109
                     /locus_tag="AC16O9_13885"
     CDS             17516..18109
                     /locus_tag="AC16O9_13885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6517740.1"
                     /translation="MRPVTRRETVHGPAGGIECAVDEPKGAARGVALIAHPHPLYGGT
                     LDNKVVQTLSRAMVELGYAAWRPNFRGVGRSEGRYDEGRGEVEDLAAVLEHVRAERPV
                     LAGFSFGAAMQAKLAGRVAPERMVLVGIAVNHFEAPTVPANTLLIHGENDVTVPIAAV
                     RDWAEPQGLALVVVPGADHFFHRKLPELRSAVTANWT"
     gene            18111..18524
                     /locus_tag="AC16O9_13890"
     CDS             18111..18524
                     /locus_tag="AC16O9_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR6517741.1"
                     /translation="MLWVKSLHIIFIVTWFAGLFYLPRLFVYHALSPDTLSRERFKLM
                     ERKLYWGIMTPSGVLALFFGLWLWLGWGFGGTWLTAKLALAALLAAYHLWCGKLMRDF
                     RDERNRRSHVWFRWFNEFPVLVLFAAVFLAVFKPA"
     gene            18502..19659
                     /locus_tag="AC16O9_13895"
     CDS             18502..19659
                     /locus_tag="AC16O9_13895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR6517742.1"
                     /translation="MPSSSPPEAFFASCPRGLEPVLAAELSSFGAASPAPVAGGVAFG
                     GSWETCYRANLWSRIASRVLWRVARFDYASEDEVYDAARAVNWFDLFDVNRTLRVSVT
                     AQKSPLKSLEFATLRVKDAVCDRFRDDVGRRPDVDRAAPEVRVHLFLEEKRGTLYLDT
                     SGEGLFKRGWRMQTVEAPLRENLAAGIVMLTGWKFDQPLLDPMCGGATLLVEAAAMAR
                     GRAPGMKRSFGFEKLDSFNGALWKSLRDEAAQPAAKAPKLRLFGSDSNAAALTAARRN
                     LGAAGVERWVTLEASDVLSRKAPADSGVMIANPPYGERIGESEELARFYPKLGDALKR
                     NFAGWNCFFFTADRRMEKLIRLQTSRRTPLYNGALECRLFEFRIVAGSHRD"
     gene            complement(19656..20615)
                     /locus_tag="AC16O9_13900"
     CDS             complement(19656..20615)
                     /locus_tag="AC16O9_13900"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6517743.1"
                     /translation="MRLAFALLTAAFAAGCALSPPHVPNPQPLEPGRLPAPDTALNIP
                     RLGPCTGSPDRTIRLNSNHPVTVLVHGCNGSAGHFRSLAQLYAFHGQQAVCFSYDDRD
                     SLMVSSAQLIAALDELNGKLRGGGITLVGHSMGGLVARKALEAERSHLWRHPDPNLRL
                     ATVSAPLSGIAVASNCGLTALHWLSLGAVPGMCWMITGDNWYEITKHSDFIRKPGALL
                     PSVQRYLKVVTDERGTCRRRNAYGACVESDHIFSLAEQYHPVVDRFPQVTNVEVGAGH
                     VEIVGYKDVAPRKLLEILQREKMLAPTPPARRAALERLLTLLY"
     gene            complement(20622..20768)
                     /locus_tag="AC16O9_13905"
     CDS             complement(20622..20768)
                     /locus_tag="AC16O9_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517744.1"
                     /translation="MTLCPVALAVGCKKCPAYSACPLKGVIGDYKKPEESQPAPQAER
                     PKDR"
     gene            complement(20787..21053)
                     /locus_tag="AC16O9_13910"
     CDS             complement(20787..21053)
                     /locus_tag="AC16O9_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517745.1"
                     /translation="MHDERIKVRVTATGQTLDVVVFSKRAEVITVVLGEGVHNVRCAL
                     TPTRNGLAYAGNAMGREIVYERSQQQVRADIERLNPAVRGPRGR"
     gene            complement(21067..22005)
                     /locus_tag="AC16O9_13915"
     CDS             complement(21067..22005)
                     /locus_tag="AC16O9_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517746.1"
                     /translation="MRVLKWVLAALGVLGALVLGAGVYLYTAAPERAVRVALEYERRL
                     AGLERRELTLANGLTYVYLEGGRGAPLLLLHGFGGNKDNFTRVAKYLTPRFRVIVPDH
                     IGFGESSKPPQADYAPPAQAERLRAFARALGVSKLHLGGNSMGGHIALTYAALHPREV
                     ESLWLLSAAGVWSAPPSEVLRRVSETGENPMLVQDEDDFAHLVSLLTAKPLMIPRPFL
                     DVVAQERVRNYALEARILKQLAADSVEERIRGLAVPSLVVWGQQDRVLHPGTAGILQM
                     LLIKSDVVMMQGVGHVPMIEAPERAALDYLQFRAKL"
     gene            complement(22005..22457)
                     /locus_tag="AC16O9_13920"
     CDS             complement(22005..22457)
                     /locus_tag="AC16O9_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HIR6517747.1"
                     /translation="MGTLLAFLHHLAAFTLVATLAIEFVLIRGELGVRAARRLQLTDM
                     FFGLSSGVVLLVGLLRVFYLEKGASYYFHSWPFLAKLVLFLGVGLASIPPTLEFMSWR
                     GALRQGHAPVLSEARRRRLLRLMHLELVGIVLILACAAMTARGIGFFG"
     gene            complement(22461..22673)
                     /locus_tag="AC16O9_13925"
     CDS             complement(22461..22673)
                     /locus_tag="AC16O9_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517748.1"
                     /translation="MKKILGIALIVAGTLGLVYGGFTYTKERRDVKLGPVQLSVKAKE
                     RVNVPVWAGVGAIALGVVLILVGGRK"
     gene            complement(22681..23010)
                     /locus_tag="AC16O9_13930"
     CDS             complement(22681..23010)
                     /locus_tag="AC16O9_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517749.1"
                     /translation="MDTRCPLCGTDLGETKPARSLIARMNLDCSHCLGQLEMNVHPAE
                     TALVLASVGGAVALAALAYARQSQGLLLAALALGMAGAAAIYTVERTWLRRWPRFRPR
                     AAPPAME"
     gene            complement(23018..23341)
                     /locus_tag="AC16O9_13935"
     CDS             complement(23018..23341)
                     /locus_tag="AC16O9_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517750.1"
                     /translation="MQEAKTCPACGFLLRYEDPPDPSFSAARYVAVELLLWVALACLL
                     AFLWATPDTGEPYAVLAVIALAAWIYLRPRQRAQAAALLGRRRYRCAQCRRDFSVQDL
                     NLPGA"
     gene            complement(23357..24493)
                     /locus_tag="AC16O9_13940"
     CDS             complement(23357..24493)
                     /locus_tag="AC16O9_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR6517751.1"
                     /translation="MEREQATKFMYDLLRALVAKKGSDLFITTGYPPAMKIDGKMTPV
                     SQQGLSPQHTSMLARSIMNDKQAAEFESKKECNFAINPAGIGRFRVNTFVQQGNVGMV
                     LRVITTTIPNFEDLKLPPVLRDVAMTKRGLVIFVGGTGSGKSTSLAAMIGYRNENSHG
                     HIITIEDPVEYVHPHKNCIITQREVGVDTDSWEAALKNTLRQAPDVILIGEIRDREVM
                     EHAIAFAETGHLCLGTLHANNSNQAMDRIINFFPEERRQQLLMDLSLNLKAVISQRLI
                     PVKEGRGRAAAVEVLLNSPLIGDLIFKGEVHGIKEIMKKSRELGMQTFDQALFDLYEQ
                     GRIGYEDALRFADSTNEVRLAIKLHGKEAKDKDLSKGIEHLDIV"
     gene            complement(24522..25565)
                     /locus_tag="AC16O9_13945"
     CDS             complement(24522..25565)
                     /locus_tag="AC16O9_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR6517752.1"
                     /translation="MDLTELLAFVVKNKASDLHLSSGLPPMVRVHGDVKRINVPALEH
                     KDVHSMIYDIMNDGQRKFYEENLECDFSFAVPNLARFRVNAFVQQRGAAAVLRTIPTK
                     VLSLEELKCPKIFTEISEYPRGIVLVTGPTGSGKSTTLAAMINHINENEEGHILTIED
                     PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDSILVGEMRDLETIRLALTGA
                     ETGHLVFGTLHTSSAAKTVDRIVDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGAG
                     RVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGAQIGMQTLDQNLQDLVRRNVVSMGE
                     ARGKAMNKDAFAG"
     gene            25598..26284
                     /locus_tag="AC16O9_13950"
     CDS             25598..26284
                     /locus_tag="AC16O9_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR6517753.1"
                     /translation="MGSIAQNLQAVKSRIAEAERVAGRAPGVVALLAVSKTHAPERIG
                     EACAAGQRAFGENYVQEALDKMAHLAALPLEWHLVGPLQSNKTRAVAERFDWVHTVDR
                     EKTARRLAEQRPAGRAPLNVLIQVNVSGEASKSGVAPAQAAALAAAIAPLKNLRLRGL
                     MAIPEPGAPPSRFTEIGALFEGLRGEFGLDTLSLGMSEDLEAAIAAGSTMVRIGTAIF
                     GERRKVRHAA"
     gene            26281..27093
                     /gene="proC"
                     /locus_tag="AC16O9_13955"
     CDS             26281..27093
                     /gene="proC"
                     /locus_tag="AC16O9_13955"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR6517754.1"
                     /translation="MRIAFVGGGNMASALIGGLIARGTRSGELSVIEPLPAARRRLAA
                     KFKVRASAAPDARTRAADTLLLAVKPQDMRAALAPLAGALAGKLVISIAAGIRLRELS
                     RWLGGHAKLVRCMPNTPALIGAGITGLYAPPGVTAAEKRRAERILGAVGATVWVGNEA
                     LLDPVTAVSGSGPAYVFWFIEQLAAAGEALGLAPGVSRKLALRTVLGAAQLAAGSREP
                     PGVLRERVTSKGGTTAAALKVFEDEGLGERFRRAVAAASRRGTEIGDELGRD"
     gene            27093..>27391
                     /locus_tag="AC16O9_13960"
     CDS             27093..>27391
                     /locus_tag="AC16O9_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6517755.1"
                     /translation="MLVQIGQFLVDVVGSFFVFILLARFHFQWLRVPFRNPIGEFMIA
                     VTNWIVLPARRVIPPLAGLDLATLIAAWLLQALALTLLVAMAGGSGSTLVFVASA"
BASE COUNT         3819 a         9529 c         9686 g         4357 t
ORIGIN      
        1 cagtcgagcg agcgctgcag gttcgcgccc accgcgccgc cgatctcgcc ggcgtggcgc
       61 atcacctcgt cgagcgagcc gtactgctgg atccatttcg ccgccgtctt cgggccgacc
      121 ttgtcgacgc cggggacgtt gtccacggcg tcgccggtga gcgtcaggta gtcgacgatt
      181 tgtcccggcg caacgccgaa cttgcgcttc acgccctcga cgtcgagcgt ctcgttcgac
      241 atggtgttga cgagcgtcac gtgctcgtcg acgagctgcg tcaggtcctt gtcgccggtg
      301 gacaccaccg tgcgccagcc gcgctgcgtc gcctcggcgg cgagcgtgcc gatgacgtcg
      361 tcggcttcca cgccttcgac cgcgagcacc ggccagccga gcgcggcgat cgcttccttc
      421 agcggctcga tctgcgccgc gagcggctcg ggcatcgacg ggcggttggc cttgtactgc
      481 ggatagaccg cctcgcggaa ggtggggccc cgcgcgtcga acacgcaggc gagcgcgtcg
      541 gccttgtagt cctcggccag tttccttagc atgctcagga cgccgcggat cgcgccggtc
      601 ggctcgccgc gcgggctctg cagctcgggc agcgcgtgga acgcccggta gaggtacgac
      661 gagccgtcga cgagcagcag cgtcttttgc ggagctttcc ctgacacgat gagcaacgac
      721 aagctgccca ggcctgcgag ccgcgcgccg gaaatcagcg cgcgcgaggc atggcgggtg
      781 ttcggcatta tggcagagtt cgtcgagtcg accgagcgcc tcgccgacat ccgccccgcg
      841 gtctcgatgt tcggcagcgc gcgcgtggcg ccgggctcgc cctactaccg tctcgccgag
      901 gaggtctcgc gcctgctctc ggacgcgggc ttcgcggtga tctcgggcgg cggccccggc
      961 gtcatggaag ccgccaacaa gggcgcgcac gccggcatct cgcccgccat cggcctcaac
     1021 atcgatctgc cgcgcgagca gagcggcaac cgcttccagg acatcagcct gcgcttcaag
     1081 catttcttcg cgcgcaaggt gatgttcgtg aaattcgcca ctgccttcat catcctgccc
     1141 ggcggctacg gcacgctcga cgagctgttc gaggcgctca cgttggtgca gaccggcaag
     1201 acgcgcaaga tgccgatcat cctgatgcac gcgccctact ggcgcgggct gctggcgtgg
     1261 atccgcgagc gcctggtggc cgaggtgatg atcgaccccg gcgacgcgga cctgttgcag
     1321 gtgatcgacg agccgcgcgc ggtggtcgag gccatcttct cctactacga gaagcgcggc
     1381 ttcgagccct cgccggccga gcgcgaggtg ctgctcaacc tctagtaaaa tcgcccgcat
     1441 gcgccgcgat gcgctcgacc ccggactgcg cgcgcagccg gtcacgcacc tcgactgact
     1501 tgtcggtcta taccgccgtc tcgcagcccg agctcgcggc ctggctgcgc aactactccg
     1561 tgggcgcgct cgaggccctc gagccgatcg aaaccggtat agagaacacg aactacttcg
     1621 tcaccaccgc gcaaggccgc tacgtgctca cgctgttcga gcgcctgccc gcggccgagc
     1681 tgccgttcta tctcaatctg atggcgcatc ttgcgcgcca cggcatcccc tgcccggcgc
     1741 cggtcgccgg cctggacgac gcctacctcg gcgagctgaa cggcaagccc gcggcgctgg
     1801 tgacgcgact gcccgggcgc tcgctcgagc gcccggggcc ggacgaatgc gccgcgctcg
     1861 gggcgctcgt cgcgcgcatg cacctcgccg cgcgctccta cggcgcctat ctcgataatc
     1921 cgcgcgggcc gcgctggtgg cgcttggcgg cgcgcgatct cgagccgttc gtcgacgccg
     1981 gcgagcgccg gctgctggag gcggagctcg cgttccaggc gcgccagcgc tttcccgacc
     2041 tgccgcgcgg gccggtgcac gcggacctgt ttcgcgacaa cgcgctgttc gagggaccgc
     2101 gcctttcggg ggtgttcgac ttctacttcg ccggtgtcga ctgcctgctc tacgatgtgg
     2161 cggtgtgcgc gaatgactgg tgcctcgccg atccggcgcg cgaccggcgc ctggacccgg
     2221 cgcgcctgca ggcctttctc ggcgcctatg cggcgctgcg cccgttcagc gccgaggagc
     2281 gcgatgcgtg gccggcgatg ctgcgcgcgg cggcgctgcg cttctggctc tcgcgcctgc
     2341 acgacctgca cctgccgcgc ccgggcgagc tggtgcacgc gcacgacccc gcgcactttc
     2401 gcgacattct cgagcgccgc gcggccgagg cgccgccctg gctcgccgcg ccatgaacgc
     2461 gcgcctggtc tcgcccatgc agggcgcgcg ctggctggcc gcgggctggc gcatgttccg
     2521 cgccgcgccc ctcggctggc tggtgctcgt tttcgcctat ctgatggcga cgatgctgct
     2581 gagcgcgttt ccgctcatcg ggccggcggc ggtgtcggtg ctgatgccgg ccttctcggt
     2641 gggattcatg gcggcgagcc gcgccgcggc gcggcgtcag gcggtggagc tgccgatgct
     2701 gttcgccgga ttccgcgagc gcctgccgca gcagctggtg ctggggtgcg tctatgcggc
     2761 gggcttcgcc gcggcgcttg ccggctcggc gctgcttgac gacggcgcgc tgctcggcgt
     2821 gctgttctcg ccgcagcggg tccaggcgga gggcgggccc ggcgatgcgc tgctcctcgg
     2881 cgtgctcgcg tcggccctga tctacctgcc caccatgatg atgctgtggt tcgcgccggt
     2941 gctggtggcc tggcatgcgg tgcacccggc caaggcgctg ttctacagcc tggtggcgtt
     3001 ctggatgaac cgcacggcgt tcatcgccta cgcgctcgcg ctcgctctgg tgctctttct
     3061 gtcgatgggc tcggtggtcc tgctcgccgc gctgatgccc ggcgaggccc cggccttcaa
     3121 tccgcgctcg ctcgttttcc cgatggcgct cgtcatcctg ccgacgctct tcgcgagcta
     3181 ctacgcgagc taccaggacg tgttcggcgc cgccggagaa ccctgatgca gccccggctg
     3241 cgcgagatcc cgtacaacta cacgtcgttc tcggaccgcg aaatcgtgct gcggctgctc
     3301 ggcgccgagg gctggcggct gctcgccgag ctgcgcgccg agcggcgcac gggacgctcg
     3361 gcgcgcatgc tgtacgaggc gctgggcgag atgtgggtca tccggcgcaa cccctacctc
     3421 gaggacggcc tgatcgcgga ccggcgcgcg cgccgccagc tgctcgaggg catgcgccgg
     3481 cgcctggcgg agatcgaggc gcggcgcgcc ggcaacgaga aggtgggcga gctgctggcg
     3541 ctggccggcg cagcgctcga gcgcttcgct gcatcgttcg aggagacgcg cgcgctgcgc
     3601 gccagggtgc tgaagcgcct gcggcgcgtg acgcggcgcg acaacatcgc cttcgaccct
     3661 tacgcgcggg tcgcgcacgt caccgacgcg accgactggc gcgtggaata cccgttcgtc
     3721 gtcgtctcgc ccgacaccga ggaagaggtg ctgatgctgg tgcgcgcgct cggcgcgctc
     3781 ggcctgacca tcatcccgcg cggcggcggc accggctata ccggcggtgc ggtgccgctc
     3841 acgccgcgcg ccgcggtcat caacaccgag aagctcgaca cgctgggcgc ggtggagtgg
     3901 ctggagctgc cgggcgccgg acgcaagacg cccacggtgc gcgccggcgc cggcgtggtg
     3961 acgcggcgcg tgatggacgc ggcggaggag gcggggctcg tcttcgcggt cgatccgacg
     4021 tcggccgagg cgtcctgcgt cggcggcaac gtcgccatga atgccggcgg caagaaggcg
     4081 gtgctctggg gcaccgcggt cgacaacctc gcctggtgga agatggtgac gccgcagggc
     4141 gattggctcg agatcgagcg ctaccagcac aacttaggac ggctgcagga caccgccagc
     4201 gccacgtttc acctgcggcg catctcgccc gacggcaaga agctgctcga agagcgcgcc
     4261 atcgagctgc cggggagcgc gttccgcaag cagcggctcg gcaaggacgt caccgacaag
     4321 ttcctcggcg ggcttcccgg cgtgcaaaag gagggctgcg acggcatcat cgtcgccgcg
     4381 cgcttcatcc tgcaccgcat gcctgcgcac acgcgcacgg tgtgcctgga attcttcgca
     4441 ccggcggccg aggccgtgcc ggcgatcgta gagatccgcg accacgtcgc gcgcctcgcg
     4501 ggcgtgcagc tcgccgggct cgagcacctc gacgagcgct acgtgaaggc ggtgggctac
     4561 ggcaccaagt cgcgccgcgg gcagcggccg cggatggtcc tgatcggcga cctggtgggc
     4621 gcgaacgtcg acgaggtggc gagcgccgcg tcctcggtgg tgcgcatcgc caacgcgcgc
     4681 ggcggcgagg gctttgtcgc cgtgtcggcc gaggcgcgca agcgcttctg gctcgaccgc
     4741 gcgcgcaccg cggccatcgc gcgccacacc aacgccttca agatcaacga ggacgtggtg
     4801 atcccgctgg agcatctcgg cgcctacagc gacggcgtcg agcgcatcaa catcgagtgc
     4861 tcgatcgaca acaagctcgc gctgctcgat gcgctcgagg aatatttcca gggcgcgctg
     4921 ccgctggtgc agggcgagga ggcgctgccg cgaccggagc tgctcggcga ccgcgcgcag
     4981 caggcgctcg atctcctcgc cgcggtgcgc acgcgctggc agcggctgag gaacggcatc
     5041 gatttcgagt tcgccaagct gcaggcgcgc accgagcgcc tgtcatggaa gaccgaggtg
     5101 cgcgcggagc tcgcgcgcat ctttgccgga ccgccctacg cgaagatcct cgaggaggcc
     5161 gacgcgctgc acaagcgcgt gcggcgcggc cgcgtgttcg tcgcgctgca catgcacgcc
     5221 ggcgacggca acgtgcacag caacattccg gtgaactcgg acgactacgc catgctgcag
     5281 cgcgcgacgc aggtggtggc gcgcctgatg gcgctcgcca agtccctcgg cggcgtggtc
     5341 tcgggcgagc acggcatcgg catcaccaag ctcgaatacc tcgagcccgc ggagctcgaa
     5401 gcgttccgcg cctacaagga ggccgttgat cccgaggggc gcttcaacgc cggcaagctg
     5461 ctggccggcg gggggctgga gaacgcctac acgccgagct tctcgctgat cggcgccgag
     5521 tcgctgatcc tggaggagac cgacctcggt gccgtgaccc gctcggtcaa ggattgcctg
     5581 cgttgcggca agtgcaagcc ggcgtgcgcc acgcatgtgc cgcgcgccaa cctgctctac
     5641 tcgccgcgcg acaagatcct cgccgcctcg ctcctcatcg aagcgatcct ctatgaggag
     5701 cagacgcgcc gcggcgtggc gcgcgtgcac tacgacgcct tcgccgacgt cgccgagcac
     5761 tgcacggtgt gccacaagtg cgccagtccc tgtccggtgg acatcgattt cggcgacgtg
     5821 tccatcgcca tgcgcaacct cctgcgccgc gccgggaaag cgcgcttcaa tccgggcacg
     5881 cgcgcctcga tgctgcttct caccgccacc gatccgcgcg ccgtgcgcgc ggcgcgcctc
     5941 gccctggtcg gctggggctt ccggctgcag cgcgcagcgc atcgcctcct ccgccttctg
     6001 tggcgcgggc agaccgcgcg cccgcctgcc accaccgggc agccgccgct gcgcgcccag
     6061 gtggtgcact tcgtcaacaa ggcgctgccc ggcgctctgc cgaagaagac ggcgcgcgcg
     6121 ctccttgaca tcgaggacgc gaagatcgtg cccatcgtgc gcgatccggc caaggtggga
     6181 gaagactcgg acgcggtgtt ctacttcccg ggctgcggct cggagcgcct gttcagccag
     6241 gtaagcctcg ccgcgcaggc catgctctac gaggtgggcg cgcagtgcgt gctgccgccg
     6301 ggatacctgt gctgcggcta tccgcagagc gccgccggcg agcaggacaa gggcgagagg
     6361 atcatcaccg ccaaccgcgt gctgttccac cgcctggcga ccacgctcaa ctacctcgac
     6421 atccgcaccg tgatcgtgtc gtgcggcacc tgcctcgacc agctggagaa gtacgagttc
     6481 gagcgcatct ttcccggctc gaggctgatc gacatccacg aatacctcct ggagaagggc
     6541 gtcaagctcg aaggcgtcga gggccagcgc tatctgtacc acgacccctg ccatacgccg
     6601 atgaagctgc gcgcgccgct ggagacggtg cgcgcgctga ccggcggcgc cgtgcagccc
     6661 gccgcgcgct gctgcggcga gtctggcacg ctcgccacga cgcgccccga catctcgacg
     6721 caggtgcgct atcgcaagga agaggagctc aagcgcgacg tggcggcgct cccgggcgac
     6781 ggcccggtga aggtgctcac gtcgtgcccg tcgtgcctgc agggccttgc acgcttcgac
     6841 gcggacgcgg gcacgcaggc cgattacatc gtggtcgaga tggcgcggcg cctgctgggc
     6901 gagaactggc tgccggaatt cgtcggcaag gcgaacaccg gcggcatcga gcgcgtgctg
     6961 ctgtagagtt tgccgcgatg agcccgccga gcgccttgcc gccgcccgcg atcgatcccg
     7021 cggcgcagcc cgagttcagc gacgcggcga gcgccaaggc ctggctcgag cacgtgccgc
     7081 tcgccaacgt gtcggcggcg cagcaccagc tgctcgtgca gatcgaggag ttcaaccgct
     7141 acgcggccgg gccggaggcc cgcttcgccg tgatggagac gctgcgcgag gcggtgaact
     7201 tcgtgcagat cgagcaggcc aagcgcttca ccaaccgcgc gctgccgatg caggcggccg
     7261 aggcgaccct gttcgaagaa acgatcgagc tgtgggagca gatgcgcctg ggctacctgc
     7321 gctgcctgga ggcggatgcc gcggcgctgc gcggcaagcg cgcgctcctc gcgcagcgcg
     7381 cgctcgccta ctcggggctg cgcatgttcc accactaccg cgcttaccgg caggtcccgg
     7441 cgcgcgactg gcgcaacctg caccagacct acgcgcgcgc cgagcatctg ggcgtgaccg
     7501 agacggcggt gaaagactac ctcaaccgcg acgtgcacga cacctcgccg cgcatcgcct
     7561 acctgcgcgc cacgctgatg ggcatgtgca acccgcacga gctgacgccg cgccagctca
     7621 cgttcgtcgc ctacctgctc gagcgctggg ccgacaaggt ggagatctcg ggcgcgccgg
     7681 tcgacgaggg cgatctgccg ccgctcagcg tcgacctcgc cggcgagcgc tgcgccgagc
     7741 gcggcaccgc ggcgggcgcc gagctgcgct acctggatgc gcggcgattg gcgaagagca
     7801 tccgcaaccg cgtcgcgctg ctcagaaagg gcgagtcgcc ggcgcggctc gcgctcggtg
     7861 aggactgcgt gcagccctcg tgcgagcagc tgctcgtgtt cctgtaccgg cagtggtgcc
     7921 aggcgcgccc cgcgcgcggc gtggaccgcg accgcgcgca agacaaggtg caggcctgca
     7981 ccggccttgc cgccatccac tattacgtct cggggcgggt gttccgcgtc acggccacct
     8041 tcaaggagct caccgcgagg cagcgcgacg agatcgccac ctttggccgc gtcagcacgc
     8101 gcgacgagga cgactacagc gtggcgcagg gatttctgct cgagcagtgg cagctcgagg
     8161 acgagagcgc gcagggcctg aagatgctgc gccgcgccgg cagtcccggg cagcgcatgg
     8221 cggccggcca gctcgtcggt gtgcggcatg cagacgcaaa gagcttccag atcggccagg
     8281 tgcgctggct gctcgccacc gaggccgggg acctggtctg cggcgtgaag ctgctgcccg
     8341 ggctgccgct cgccggcgcg gtgcgcggca ccggcgtcaa cgccgccgac gagaagtacg
     8401 tgcaggccgt ggcgctcgcc gcggtgccgg cgctcaaggc gccgccgact ctggtcctgg
     8461 cgccgggctg gttccggccc aagcgcgtca tcgaggccgc gatcccgaag ccgatgcgcg
     8521 cgcggctgct ggaagtgctc gagcgcggca acgatttcga gcgcgtcgcc tacgaggtgt
     8581 tgccggacta ggccaccgcc cgcgccgggc ccgccggcgc caagcgatcg gcggggaatt
     8641 cggcgcgaaa cgtcgagccc ttcccgaacg cgctttccac ctcgaggctc gcctggtgcc
     8701 gcgtcagcac gtgcttgacg atggcgagcc ccaagccggt gccgcccgag tcgcgtgagc
     8761 gcccctggtc gacgcggtag aagcgctcgg tgagccgcgg gatgtggcgc gtctcgacgc
     8821 cgatgcccgt gtcctgcacg gcgaagatgc cgcgcttgcc ctcgagccgc caggacagcg
     8881 tgatcgtgcc tcccggtggg gtgtagttca ccgcgttgga aacgaggttg gcgaacgcgc
     8941 tgtgcagctc gcgcgcgctg ccggtgagca gggcgtcgtg ctcgatgcgg ctcacgacct
     9001 ggtggcgccc ggccgacagt gcgcgcgcct cctccacgag ccgctccacc aacgcgcgca
     9061 tgtcgatggc ctgctcgtcg gccggcgccg ggctcgattc cagcgccgag agcgtgagca
     9121 ggtcctcgat tagctgctgc atgcgccgcg cctgctcggc catcaggccg aggtagcgcc
     9181 ggcgctgcgc ggggctcatc tcgatttcgg cctcacccag cgtctcgacg aagcccgcga
     9241 gcacggtgat cggcgtcttc agctcgtgcg agacgttggc gacgaagtcg cggcgcatgg
     9301 tctcgagctt ttcctgcgcc gtgatgtcgc gcgagatcag cagcttttgc tcgtcgccga
     9361 actcgatcag gcgcaccagg agcaccgttt cgccgcgtac gccgcgcagc acgagcggct
     9421 cgtcgaacct gcccgcctcg agccaggcga tgaaggccgg gtggcgcatg aagttcgcca
     9481 ccgggcgccc caggtcgcgg cgcgcgtcga tgctgaagtg ccgcgccgcc gtcggattgg
     9541 cccactcgat gtggtactcg cggtcgagaa tcaccacgcc gtcgggcagc gcgcggccgg
     9601 cgtcgcgaaa gcgcgccagc gccgcgcgca ggcgctcgcg ctcgtcgtcg cggtccttga
     9661 ggtgccggtg cagcgtggcg aacgcgtctt cccacaggcc gcgccccggg ggcagcgcgt
     9721 cgcgcgaggg ccgcgagagc cagcgcagga acgccgcgag ctgcaccgtg tggaacagga
     9781 gcgccagcgc gagcaccgcc gctacgccgc cccaacccca cagcaggccc gcggccgctg
     9841 ccgccactgc caccgcgagc gcgctggccc aggccggatt caagcggaaa gggcggtgag
     9901 ccggtagccc gcgccgcgca cggtctcgat caggcggtcg tggcctgaag gctcgaggga
     9961 cttgcgcagg cgccggatat gcacgtcgac cgtgcgctcc tcgaggaaca cgtggtcgcc
    10021 ccagacctga tcgagcagct gcgcacgcga gtgcacgcgc tcggcgtggg tcatgaggaa
    10081 atggagcaag cgaaactcgg tcggaccgag ttcgagcgcg cggcttgcgc cgaacacgcg
    10141 gtgcgtctcg ggctccaggc gcagccccgc cagctccacc gggtcgtcgg tcatctgcgg
    10201 cgcgcgccgg cgcagcaccg ccttgatgcg cgccagcaac tcgcgcggcg agaacggctt
    10261 ggtgacgtag tcgtcggcgc cggcttccag gcccgcgacc ttgtcggcct cggcgccgcg
    10321 cgcggtcagc aaaatgatgg ggatcgccct ggtgcggtcc tcccggcgca tctggcgcgc
    10381 gagcgcgacc cccgacattc cgggcagcat cccgtcgatg agcagccggt cgggcagggc
    10441 ggggcgcacg atccccagcg ccccttcggc atccttggcg cggatcacct ggtggccggc
    10501 atgctcgagg ttcaccgcga tcagctcctg gatcgccggc tcgtcctcga cgagcaggat
    10561 gttggcgggc atggtgacga taattggaac gtgtcgagac cgtattgttg cagaccgccc
    10621 ggggtatcat cggtcggcca tgcccacgcc gaccgagatc cggctgcacc agaagtcgcg
    10681 gctgctggaa gtcgtcttcg acgacggccg cagcttccgc ctgccctacg agtacctgcg
    10741 cgtgttctcg ccatcggccg aggtgcgcgg ccacggtccc ggccaggaag tgctgcaggt
    10801 cggcaagcgc gaggtcgaga ttcgccaagt cgagccggtc ggctcgtacg cgatccagcc
    10861 cacgttttcc gacgggcacg cgacgggcat ctattcctgg gactacctct accagctggg
    10921 cgtcgagcag gacgcgcgct gggccgccta cctcaagcag atcgaagagg ccggagccag
    10981 cagataacgt aagcggttac ttactcatgt tcgataccga cttcggctac gagcaggtgc
    11041 acgagagcga aaagcgcgcg cgggtcggcg aggtgttcga ccaggtcgcg ccgcgctacg
    11101 acctgatgaa cgacctgatg tcgctcggcg tgcatcggct gtggaaggcc ttcgccgtga
    11161 acgtggcgcg cgtgcgcgcg ggcgatcgcg tgctcgatat cgcctcgggc agcggcgacc
    11221 tggcgcgggt cttcgcgcgc cgggccggaa cgaccgggga ggtctgggcc accgatatca
    11281 accgccgcat gctcgagcgc ggccgcgacc ggctgctcga cgccggcctg cggctgcccg
    11341 ccgtgcagtg cgatgccgag cgcctgccgt ttcccgcggg gtacttcgat tgcgtcgcgg
    11401 tggctttcgg cctacgcaac atgacccaca aggacgccgc gctggccgag atggcgcgcg
    11461 tattgcggcc cggcggccgc ctggtggtgc tggaattctc gcgggtctgg aagccgctcg
    11521 agaagcccta cgcgctctat tcgttcagcg tgctgccgtg gctcggcaag caggtggcgg
    11581 gctccggcga cgcctaccgc tatctcgccg agtcgatccg caggcatccg gaccaggaca
    11641 cgctcgccgc gatgatggaa cgcgccggtc ttgcggcggt cgaatacttc aatctcagcg
    11701 caggcgtcgc cgcagtccat cgcggtgtca agctgggtta gcatcgcagc gcagttcgac
    11761 aactttacgc aaaggagcgt gcgcttcatg agcaaggggt ggttcgggat cacggcagcc
    11821 gtgctgctgg gactggtgat cgcaatgccg gaagcggagg cgcgccgcct gggcggcgcg
    11881 cgctcgctcg gcgtgcaacg taacgtcacg gcgccgcccg cggccgtgcc ggccaagccg
    11941 gcgcaggccg cgccggcggc acctgcagcg ggcacgggca gcaagctcgc gccgatgctc
    12001 ggcggactcg ccctcggcgg gctgctcggc gctttcttcg ccggcaatcc ggtcctcggc
    12061 acgctgctta ccgcgctcct cgtcggcatg ctggtgtttg caggcatcgc cctggtgcgc
    12121 gtgctgcgcg cgccgcgcac cgcaccacca gcgttccagt acgcgggcct gggcagcgag
    12181 acggtggccg cgccgccgcc gtcgcaggcc gcggggttgg aggcgcgcga gccgagcagc
    12241 ccggcgcgcg cgcacctgcc cgcgggcttc gacgccgcag ggttcctgcg cgccgccaag
    12301 gagaacttca tccgcctgca aatcgcgaac gacagcggcg agctcgaagc gctgcgcgac
    12361 atgaccacca ccggcatgtt cgaggcgctc cgcaacgacg ccggcgcggg gcagcaaacc
    12421 gacgtggtga cgctgagcgc cgacctgctc gaggtgacga ccgagggcgg ccggcactgg
    12481 gcgagcgtgc gcttttcggg cctggtgcgg gaggcacccg gggcggagcc cgtgagcttc
    12541 gaggaggtgt ggaacctgac gaagcccgcc gacggctcca gcgggtggct gctggccggc
    12601 atccagcaga tgcactgatc gctctatact ccggaggggc caccctaggg tggccctttt
    12661 tgttttcatg gccacgctcg aatccccgct ggtactggcg ctgaaccggc tgctcgaggc
    12721 cgagggctgg gcgcgcgagc gcctggcgcc ctttgccgga gaaacggtgg aactgcgcgc
    12781 gccgccgctg ccggcgctgc gcttttccat cgtcgagggc ggccggctcg cgcccggcac
    12841 tgccgcgagc ctcacgctga cgctgcggcc cgattcgctg ccggcgctgc tgcggggcga
    12901 ggagcatttc atgcgcaccg tcggcatcga cggcaacgcg caactggcgc gcgaagtcct
    12961 gcatctcgcg cgccacctgc gctgggacgc cgaggaggat ctgtcgcgcc tggtgggcga
    13021 cgcggccgcg caccgcgcgg tgatgctggc gcgcgatttc gccgcctggc agctcgacgc
    13081 cggtcgccgg ctcgccgagg ccttcatgga gtacgcgcag gaggaagggc gcctgctcgc
    13141 gccgcgcgcc gggtacgcgg ccttcgccgc cgaggtggcg cagctgcgcg acgcgctgga
    13201 gcgcctggaa aagcgcatcg ggcgccttgc atgaggcggc ttgcgcgcat cgtctgggtg
    13261 gcgctccggt tccggctgta tcgcttctgg ccgcgtttcg cgcgggccgc cgaccccgag
    13321 cgcgcagtgc ggctgcgccg cgcgctggag acgctcgggc cgatcttcgt caagttcggg
    13381 caggtgctct ccacgcgtcg cgacctcctg ccgctcgaca tcgccgacga gctggcaaag
    13441 ctccaggatc gggtgccgcc gttcgacccg gtactggcgc gcgccgagat cgagcgcggc
    13501 atcggcaagc cgatcgacgc ggtgttctcg agcttcgagc gcgaggcggt ggcgagcgcc
    13561 tccattgccc aggtgcacct cgcgcggctc cacgacggcg ccgaggtggc ggtgaaggtg
    13621 ctgcgcccgg gcgtcgagcg cgccatcgcg cgcgacgtgg cgctgctcga aaccgcggca
    13681 cggctggtgg agctgctgtg ggccgatggc cggcgcctca agccgcgcga ggtggtggcg
    13741 gagttcgcgc gccacctgga ggaggagctg gacctattgc gcgaggcggc gagcgcgagc
    13801 cagctgcggc gcaacttcga ggcttcggtg ctgctcgccg tgccgcgcgt gtactgggac
    13861 tggtgcagcc agcgcgtgat ggtgatggag cgcatgcacg gcacgccggt ctcccaggtc
    13921 gagcgcctgc gcgagaaggg cgtcgacatc ccgcgcctgg cgcgcgccgg cgtcgagatc
    13981 ttcttcaccc aggtatttcg cgacggcttc tttcacgccg acatgcaccc ggggaacatc
    14041 ctcgtcaccg acgagggcca gtacgtcgcg ctcgacttcg gcatcatggg cacgctgacc
    14101 gaaaccgaca agaactacct ggcgcagaac tttctcgcct tcttcaaacg cgactaccgg
    14161 cgcgtggcgc aggcgcacct ggatgcgggc tgggtgcccg agggcacgcg catcgacgat
    14221 ttcgagggcg cgatccgcgc cgtgtgcgag ccgatcttcg cgcgcccgct gaaggagatc
    14281 tacttcggca agctgctgct gcgcctgttc cagacctcgc gccgcttcaa cgtcgagatc
    14341 cagccgcaac tggtgctgct gcaaaagacg ctgctcaaca tcgaaggcct cgggcgcacg
    14401 ctcgatcccg acctcgacct ctggcagacg gcgaagccct acctcgagcg ctggatgagc
    14461 gagcagatcg gctggcgcgg cttggcgcgc gcgctcgccc aggaagcgcc cacctgggcg
    14521 gcgacgctgc cgcagctgcc gcgactcgcg caccggctgc tggccgaaga ccggctcggc
    14581 gcgctggagc gcgcgctggc gcggatcggc gagcagaacg tgcgtcgcaa tcgcctgctc
    14641 gcggcgatcg cggcgctgct tgccgcggcg ctgattctca ttatgatggc ggcggcatgc
    14701 tgatcgcctt cgttgcgctc tatctcgtgg cctcgatcgc catcggcttg tgggcggcgc
    14761 ggcgcgtgca caactcgcgc gactacgtgg tcgccggccg cagcctgccg ctctacatga
    14821 gcttcgcgct ggtgttcgcg acctggttcg gcgccgagac cgtgctcggc gtctcggcca
    14881 ccttcctgaa ggacggcatg ggtggcatcg tcgccgaccc gttcggcttt tccttctgcc
    14941 tggtgctgat tgcgctgttt ttcgcgcgcg cgttctaccg cctgaacctg ctcaccatcg
    15001 gcgacttcta ccgcaagcgc tacaacaagc ccgtcgaggt cgccaccagc gtgtgcatca
    15061 cgctgtcgta cctcggctgg acctcggcgc agttcaccgc gcttgggttc gtgttccacg
    15121 cgctctcggg cggcgcgatt cccctgcact ggggcatcgc gctgggcgcc ggctgcgtgc
    15181 ttgtctacac attgttcggc ggcatgttct ccatcgccta cacggacctg ttccagacca
    15241 ccatcatcat cgtcggcctg atctacctcg cgtttctgct cggcggcatg gcgggcgggc
    15301 cgggcgcggt gctggcgcac gcggcgcagg ccgacaagtt ccagttttgg cccaagcccg
    15361 agctgaagga gatcctcgcc ttcgtcgccg ccttcctcac ggccgcgctc ggctcgattc
    15421 cgcagcagga cgtgttccag cgcgtgactt cggccaaaga cgccgacacc gcggtgcgcg
    15481 gcacgatgct cggcggcatc gtctacttct tcatggcgtt cgtgccgatc taccttgcct
    15541 actcggcgct gctcatcgac ccgcagatgg tggcgccgct catcgagggc gatgcgaacg
    15601 agaaccagct catcctgccc aacctgatcc tggggcacac gccggtcttc gcgcaggtga
    15661 tgttctttgg cgcgctgctc tcggcgatca tgtcctcggc ttcgggcgcg ctgctcgcgc
    15721 cgacgtcgct ctttaccgag aacgtgctgc gcccgttcct gccgaacatg agcgaccggc
    15781 agttcctgct cacgctgcgc ctcgtgctgg tgatcttcgc cctcgcggtg ctggtgttcg
    15841 cgctggagag cgacgccagc atctaccaga tggtgcagaa cgcctacaag gtgacgctgg
    15901 tctcggcgtt cgcgccgctg gtgctcggcc tgttctggaa ccgcgccacc accgcgggcg
    15961 cgctgctctc ggtggtgctc ggcctggtga cgtggctgct catggaggcc gtcgccccgg
    16021 aggcgctcgt gccgccgcag ctcgtcggtc tcgcggcgtc gttcctcggc atggtggtcg
    16081 gcagcctcgc ctcgcggccg gcgcacgcgc gggcgcaccc cggatagcgc gtgcgcggct
    16141 cgccgctcgc acgcctgctc cttttcgtct acgcgctgct cgtcgtctac gcgtcgctgc
    16201 acccgctcgc gggctggcgc gaccacggtc tcatgccgtt cgagttcctc gccgcgccgt
    16261 ggccgcggca cgtggtcctg ttcgacgtcg cggccgacgt cgtcggctac gccgcgctcg
    16321 gcgcgctcgc cgtgctcgca tggcacccgc ggctgcggcg cggccgcgcg gtgctcgcgg
    16381 ccaccgcgct gggcctggtg ctgtcgatcg tcctggagtc ggtgcagacc tatctgccga
    16441 cgcgcgtcgc gtccaacgtg gacgtgctgt gcaacacggc cggcgcgctc gccggcgcgc
    16501 tcgcgggcct cgcgttcgcg ccgctgtcgc ccgcgctgcg cggctggcgc gcgcgcctct
    16561 tcctgcccgg cacgctgtcg gacctgggcg tcgcgctgct cggcctgtgg ctgttcatgc
    16621 agctcaatcc ggcgatgctg ctgttcggcg gcggcgacct gcgcaacctg atcgctggcc
    16681 cgggcggggc ggcgcgcgcg ccggagttct tcgccgccat cgaggcgatc accgccgccg
    16741 ccaacctcgc tgcgctggcg ctgcttgcct cggtgatcgt cgcgcccggc gcgccggtgc
    16801 ggcggctcgt gctgctcctg ctcacggccg cgcttgccgt gaagacgctt gcgttcacga
    16861 tcatcctgcg cgcgcaggac gtcttcgcct ggctcacgcc gggagcgcag cgcggcctcg
    16921 ccgcggggct cgtgcttgcg atgctcgcgg tggcgctgcc gcgcgtggcg cgcatggcgc
    16981 tcgccgcggt cctgctcacg gcggccgccg tgctggtcaa tcacgcgcca cccaaccctt
    17041 atctggccga gattctcaag ctctggcagc aggggcactt cctcaacttc aacggcctca
    17101 cgcgcctggt ggccacgctg tggcccttcg ccgccgtcgc ctacctgatc gtgctggcgg
    17161 cgcggcgccg cgacgagggg ctgctgtaga attcgcgccc catgtcctac tacgagcgcc
    17221 acgtcttctt ctgctgcaac cggcgcgacg cctccgaccc gcgcggctgc tgcagcgcca
    17281 agggcgcttc ggacatgcgc gactacgcga agcagcgcgt caaggagctc aagctcgcgg
    17341 gcgcgggcaa ggtgcgcatc aaccaggccg ggtgcctcga tcgctgcgaa caggggccct
    17401 gcgccgtggt gtatccggat gccgtctggt acacctacgt ggaccgcggc gacatcgacg
    17461 agatcatcga ggagcacctg cggcacggcc gcatcgtcga gcgcctgagg atctgatgcg
    17521 tccggtgaca cggcgcgaga cggtccacgg acccgccggc ggcatcgagt gcgccgtgga
    17581 cgagccgaaa ggcgcggcgc gcggcgtcgc gctgatcgcg catccgcacc cgctgtacgg
    17641 cggcacgctt gacaacaagg tggtgcagac tttgtcgcgg gccatggtcg agctgggcta
    17701 cgcagcctgg cggccgaact ttcgcggcgt cggcagaagc gaaggccgct atgacgaggg
    17761 tcgcggcgag gtcgaggacc tggcggcagt gctcgagcat gtgcgggcgg agcgcccggt
    17821 actggccggg ttttcctttg gcgcggcgat gcaggcgaag ctcgcggggc gcgtcgcgcc
    17881 ggagcgcatg gtgctggtcg gcatcgcggt aaaccatttc gaggcgccca ccgtgcccgc
    17941 caataccctg ctgatccacg gcgagaacga cgtcacggtg ccgatcgcgg cggtgcgcga
    18001 ctgggccgag ccgcagggcc tggcgctcgt cgtcgtgccg ggggcggacc acttcttcca
    18061 ccgcaagctg cccgagttga gaagcgcggt caccgcgaac tggacctgac atgctctggg
    18121 tcaagtcgct gcacatcatc ttcatcgtca cgtggttcgc gggcctgttc tacctgccgc
    18181 ggctcttcgt ctatcacgcg ctgtcccccg acacgctgtc gcgcgagcgc ttcaagctga
    18241 tggagcgcaa gctctattgg ggcatcatga cgccgagcgg cgtattggcg ctctttttcg
    18301 gcctgtggct gtggctcggc tggggcttcg gcgggacatg gctcaccgcc aagctggcgc
    18361 tcgccgcgct gctcgccgcc taccacctgt ggtgcggcaa gctgatgcgc gacttccgcg
    18421 acgagcgcaa ccgccgctcg cacgtctggt tccgctggtt caacgagttt ccggtgctgg
    18481 tgctcttcgc cgcggtcttc cttgccgtct tcaagcccgc ctgaggcgtt cttcgcctcc
    18541 tgcccccggg ggctcgagcc ggtgctcgcc gcggagctga gctccttcgg cgctgcctcg
    18601 ccggcgcctg tcgcgggcgg ggtcgcgttc ggcggcagct gggaaacctg ctaccgcgcg
    18661 aacctctggt cacgcattgc ctcgcgcgtg ctatggcgcg tggcgaggtt cgactacgca
    18721 tcggaggacg aggtctacga cgcggcgcgc gcggtgaact ggttcgacct gttcgacgtg
    18781 aaccggacgc tgcgcgtctc cgtcacggcg cagaagagcc cgctgaagag cctggagttc
    18841 gcgacgctgc gcgtgaagga cgcggtgtgc gaccgctttc gcgacgatgt cgggcgccgg
    18901 cccgacgtcg atcgcgccgc gcccgaggtg cgcgtgcacc tgttcctcga ggaaaagcgc
    18961 ggcacgctgt acctggacac ctcgggcgaa ggcctgttca agcgcggctg gcgcatgcag
    19021 acggtggaag cgccgctgcg cgagaacctg gcggcaggga tcgtgatgct gacgggctgg
    19081 aagttcgacc agccgctgct cgaccccatg tgcgggggcg cgacgctgct ggtggaggcc
    19141 gcggcgatgg cgcgcgggcg cgcgccgggg atgaagcgca gcttcggttt cgaaaagctg
    19201 gattcgttca atggcgcgct ctggaagtcg ctgcgcgatg aggcggcaca gccggcggcc
    19261 aaggcgccga agctcaggct cttcggcagc gattccaacg ccgcggcgct gaccgcggcg
    19321 cggcgcaacc tcggcgccgc gggcgtggag cgctgggtga cgctggaagc gagcgacgtg
    19381 ctctcgcgca aggcgccggc ggattctggc gtgatgatcg ccaacccgcc gtatggcgag
    19441 cgcatcggcg agtccgagga gctggcgcgc ttctacccga agctcggcga tgcgctgaag
    19501 aggaacttcg ccggctggaa ctgcttcttc ttcaccgccg atcggcgcat ggagaagctc
    19561 attcgcctgc agacctcacg gcgcacgccg ctctacaacg gcgcgctgga gtgccggctg
    19621 ttcgagttca ggatcgtggc ggggagccac cgggactagt acagcagcgt gagcaaccgc
    19681 tccagcgccg cccggcgcgc gggcggcgtc ggggcgagca tcttctctcg ctgcaggatc
    19741 tcgagaagct tgcgcggcgc gacgtccttg tagccgacga tctcgacgtg cccggcaccg
    19801 acctcgacgt tggtcacctg cggaaaccgg tcgaccaccg ggtggtattg ctcggcgagg
    19861 ctgaagatgt ggtcgctttc gacgcaggcg ccgtaggcgt tgcgccgccg gcaggtgccg
    19921 cgctcgtcgg tcaccacctt gaggtagcgc tgcacggagg gcagcagcgc gcccggcttg
    19981 cggatgaaat ccgagtgctt cgtgatttcg taccagttgt cgccggtgat catccagcac
    20041 atgccgggca ccgcgcctag gctcagccag tgcagcgccg tgaggccgca gttgctcgcc
    20101 accgcaatgc cggagagcgg cgccgagacg gtggcgagcc gcaggttcgg atcggggtgt
    20161 cgccacaggt gactgcgctc ggcctcgagc gccttgcgcg ccaccaggcc gcccatgctg
    20221 tggccgacga gcgtgatgcc gccgccgcgc agcttgccat tgagctcgtc cagcgccgcg
    20281 atgagctgcg ccgagctcac catcaggctg tcgcggtcgt cgtagctgaa gcacacggcc
    20341 tgctggccgt ggaacgcgta gagctgcgcc agcgagcgga aatgcccggc cgagccgttg
    20401 cagccgtgca ccagcacggt caccgggtgg ttcgagttga ggcggatcgt gcgatcgggc
    20461 gaacccgtgc agggacccag gcgcggaatg ttgagcgccg tgtctggcgc cgggagccgc
    20521 cccggctcga gcggctgcgg attgggcacg tgcggcggcg agagcgcgca gccggcggcg
    20581 aaggccgccg tgagcaaggc gaaggcgagc cgcatgagtc gctagcggtc cttgggtctt
    20641 tcagcctgcg gcgcaggctg gctttcctcc ggcttcttgt aatcgccgat cacgcccttc
    20701 agcgggcagg cggagtacgc cgggcacttc ttgcagccga ccgcgagggc gacggggcac
    20761 agggtcatgg cagtcctcct tcgatgtcag cggccgcggg gaccccgcac cgccgggttg
    20821 aggcgctcga tgtcggcacg cacctgctgc tggctgcgct cgtagacgat ctctcggccc
    20881 atggcgttgc cggcgtaagc caacccgttt ctcgtggggg tgagcgcgca gcgcacgttg
    20941 tgcacgccct cgcccagcac caccgtgatc acctcggcgc gcttgctgaa caccaccacg
    21001 tccagggtct ggccggtggc ggtgacgcgg accttgattc tctcgtcgtg catgccggtg
    21061 gacactctac agctttgcgc ggaactgcag gtaatccagg gccgctcttt ccggcgcctc
    21121 gatcatcggc acgtgcccca cgccctgcat catgaccacg tcggacttga tcagcagcat
    21181 ctgcaggatg ccagcggtcc cgggatgcag cacccggtcc tgctggcccc agaccacgag
    21241 cgacggcacg gcgaggccgc ggatgcgctc ctccaccgag tcggcggcga gctgcttcag
    21301 gatgcgcgcc tcgagcgcgt agttcctgac gcgctcctgc gccaccacgt cgagaaacgg
    21361 ccgcgggatc atcagcggct tcgccgtgag cagcgagacc aaatgcgcga agtcgtcctc
    21421 gtcctgcacc agcatcgggt tttcgcccgt ttccgacacc ctcctgagca cttcgctcgg
    21481 cggcgcgctc cacacgcctg cggcgctgag cagccacagg ctctcgactt ccctgggatg
    21541 cagcgcggcg taggtgaggg cgatgtgccc gcccatggag ttgccgccga ggtgcagctt
    21601 cgacacgccg agcgcgcgag cgaaggcgcg caggcgctcg gcctgcgcgg gcggcgcgta
    21661 gtcggcctgc ggcggtttgg aggattcgcc gaagccgatg tggtcgggca cgatgacgcg
    21721 aaagcgcggc gtgaggtact tggcgacgcg cgtgaagttg tccttgttgc cgccgaagcc
    21781 gtgcagcagg aggagcggcg cgccccggcc gccttccagg tagacgtagg tcaggccgtt
    21841 ggcgagcgtg agctccctgc gctcgagccc ggcgaggcgc cgctcgtatt cgagcgcgac
    21901 acgcaccgcg cgctcgggag cagcggtgta caggtacacg cccgcgccca gcacgagcgc
    21961 gcccagcacg cccagggcgg ccagcaccca cttcaggacg cgcatcagcc gaaaaagccg
    22021 atgccgcgcg ccgtcatggc ggcgcaggcg aggatcagca cgatgccgac cagctcgagg
    22081 tgcatcaggc gcagcaaccg gcggcgccgc gcctcgctca gcaccggcgc gtgcccctgg
    22141 cgcagggcgc cgcgccacga catgaactcg agcgtcggcg gaatcgacgc gaggcccacg
    22201 ccgaggaaca gcaccagctt ggcgaggaac ggccagctgt ggaagtagta gctcgcgccc
    22261 ttctcgagat agaacacgcg cagcagcccg acgagcagca ccacgcccga ggacaggccg
    22321 aagaacatgt cggtgagctg caggcgccgc gcggcgcgca cgccgagctc gccccggata
    22381 agaacgaact cgatcgccag cgtcgccacc agcgtgaacg cggcgaggtg gtgcaggaat
    22441 gcgagcagcg tgcccatcgc ctacttgcgg ccgccgacca gaatcaggac cacgccgagg
    22501 gcgatcgcgc ccacgccggc ccacaccggg acgttgacgc gctctttcgc cttcaccgac
    22561 agctgcaccg gcccgagctt cacgtcgcgc cgctccttgg tgtaggtgaa gccgccgtac
    22621 acgaggccca gggtgccggc gacgatcagg gcgatgccga ggatcttctt catggcgcca
    22681 ttattccatt gcgggaggcg ccgcgcgcgg ccggaagcgc ggccagcgcc gcagccaggt
    22741 gcgctcgacg gtgtagatcg cggccgcgcc cgccatgccg agcgcgagcg cggcgagcag
    22801 cagcccctgg ctctggcgcg cgtaggcgag ggcggcgagc gccacggcgc cgccgacgct
    22861 cgccagcacg agcgcggtct ccgccgggtg cacgttcatc tccagttgcc cgaggcaatg
    22921 cgagcagtcg aggttcatgc gtgcgatcag ggaacgggcg ggcttcgtct cgcccaggtc
    22981 ggtgccgcag agcgggcagc gggtgtccat cagtggctca ggccccgggg aggttcaggt
    23041 cctgaaccga gaagtcgcgc cggcactgcg cgcaacggta gcgccggcgc ccgagcagcg
    23101 cggcggcctg cgcgcgctgg cgcggacgca ggtagatcca ggcggcgagg gcgatgacgg
    23161 cgagcacggc gtacggctcg ccggtgtccg gcgtcgccca caggaaggcg agcaagcagg
    23221 ccagggcgac ccagagcagc agctcgaccg cgacatagcg cgcggcgctg aagctcgggt
    23281 ccggcgggtc ctcgtagcgc agcaggaagc cgcaggccgg gcaggtcttc gcctcctgca
    23341 ccgccggggg cgcgcgctac acgatgtcga gatgctcgat gcccttggac aggtccttgt
    23401 ccttggcttc cttgccgtgc agcttgatgg cgaggcgcac ctcgttggtc gaatcggcga
    23461 accgcagcgc gtcctcgtag ccgatgcgcc cctgctcgta gaggtcgaac agcgcctggt
    23521 cgaaggtctg catgcccagc tccctcgact tcttcatgat ctccttgatg ccgtgcacct
    23581 cgcccttgaa gatcaggtcg ccgatcaggg gcgaattgag cagcacctcc accgcggcgg
    23641 cgcgcccgcg cccctccttc accgggatca ggcgctgcga gatcaccgcc ttcaggttga
    23701 gcgacaggtc catcagcagc tgctggcggc gctcctcggg gaagaagttg atgatccggt
    23761 ccatcgcctg gttggagttg ttggcgtgca gcgttcccag gcacaggtga ccggtctcgg
    23821 cgaaggcgat ggcgtgctcc atcacctcgc ggtcgcggat ctcgccgatc aggatcacat
    23881 cgggcgcctg gcgcagcgtg ttcttcagcg ccgcctccca ggagtcggtg tccacgccca
    23941 cctcgcgctg cgtgatgatg cagttcttgt gcggatgcac gtactccacc gggtcctcga
    24001 tggtgatgat gtggccatgc gagttctcgt tgcggtagcc gatcatcgct gcgagcgagg
    24061 tcgacttgcc cgagcccgtg ccgccgacga agatcacgag cccgcgcttg gtcatcgcca
    24121 cgtccctcag caccggcggc agcttcaagt cctcgaagtt cggaatcgtg gtggtgatga
    24181 cgcgcagcac catgccgaca ttgccctgct gcacgaacgt gttgacgcga aagcgcccga
    24241 tgcccgccgg gttgatggcg aagttgcact ccttcttcga ttcgaactcg gctgcctgct
    24301 tgtcgttcat gatcgagcgc gcgagcatcg aggtatgctg cggcgacaac ccctgctgcg
    24361 acaccggcgt catcttgccg tcgatcttca tcgccggcgg gtagccggtg gtgatgaaca
    24421 ggtccgagcc cttcttcgcg accagggcgc gcagcaggtc gtacatgaac ttggtggctt
    24481 gttcgcgttc catcgcgttc tcctacgctg tgcttgccgt ctcagccggc aaaggcatcc
    24541 ttgttcatcg ccttgccgcg cgcctcgccc atcgacacca cgttgcgccg cacgaggtcc
    24601 tgcaggttct ggtcgagcgt ctgcatgccg atctgcgcgc cggtctggat cgccgagtac
    24661 atctgcgcga tcttgttctc gcggatcagg ttgcggatcg ccggcgtgcc gatcatgatc
    24721 tcgtgcgccg cgacgcgtcc cgcgccgtcc ttggtcttca gcagcgtctg cgaaatcacc
    24781 gcgcgcaagc tttccgagag catggcgcgc accatttcct tctcggccgc gggaaacacg
    24841 tcgacgatgc ggtccacggt cttcgccgcg ctgctcgtgt gcagcgtgcc gaacaccaag
    24901 tgtccggttt ccgcgccggt gagcgccagg cggatggtct ccaggtcgcg catctcgccc
    24961 accaggatcg agtccgggtc ctcgcgcagc gcgctcctca gcgcgttgga gaacgacagc
    25021 gtgtgcggcc cgacctcgcg ctggttgatc aggcatttct tggactcgtg cacgaactcg
    25081 atcgggtcct cgatggtgag gatgtggcct tcctcgttct cgttgatgtg gttgatcatc
    25141 gccgcgagcg tcgtcgactt gcccgagccc gtcggcccgg tcaccagcac gatgccgcgc
    25201 gggtattccg agatctcggt gaagatcttc gggcatttca gctcctcgag cgacagcacc
    25261 ttggtcggga tggtccgcag caccgcggcg gcgccgcgct gctgcacgaa ggcattgacg
    25321 cgaaagcgcg cgaggttggg cacggcgaag gagaagtcgc actccaggtt ctcctcgtag
    25381 aacttgcgct gcccgtcgtt catgatgtcg tagatcatgc tgtgcacgtc cttgtgctcc
    25441 agcgcgggga cgttgatgcg cttgacgtcg ccgtgcacgc gcaccatcgg cggcaggccc
    25501 gaggacaagt gcaggtccga ggccttgttc ttgacgacaa acgcaagcaa ttcagtgaga
    25561 tccattacaa tcccccgcga gacggtccgg gcaaagtatg gggtcaatcg cgcagaactt
    25621 gcaagccgtg aagtcacgca tcgccgaggc cgaacgcgtc gcaggacgcg ccccgggcgt
    25681 ggtggcgctg ctcgcggtgt ccaagaccca tgcgccggag cggatcggcg aggcctgcgc
    25741 cgccggccag cgcgcgttcg gcgagaacta tgtccaggag gcgctcgaca agatggcgca
    25801 cctcgccgcg ctgccgctcg aatggcacct cgtggggccg ctccagagca acaagacgcg
    25861 cgcggtggcc gagcgcttcg actgggtgca caccgtggac cgcgaaaaga ccgcgcggcg
    25921 ccttgccgag cagcgcccgg cggggcgggc gccgctcaac gtgctgatcc aggtgaacgt
    25981 ctcgggcgag gcgtccaaga gcggcgtcgc gccggcgcag gcggccgcgc tggccgcggc
    26041 gatcgcaccg ctcaagaacc tgcgtctgcg ggggttgatg gcgattccgg agcccggcgc
    26101 gccaccgtcg cgcttcaccg agatcggcgc cctgttcgag gggctgcggg gcgagttcgg
    26161 gctggacacg ctgtcgctgg gcatgagcga ggacttggag gcggcgatcg ccgcgggctc
    26221 caccatggtc aggatcggca ccgcgatctt cggggagcgg cgcaaggtga ggcacgcggc
    26281 atgaggatcg ccttcgtcgg cggcggcaac atggcgagcg ccctgattgg cgggctgatt
    26341 gccaggggca cccgctcggg cgagctctcg gtgatcgagc cgctacccgc ggcgcgccgg
    26401 cgcctggcgg cgaaattcaa ggttcgcgcg agcgcggcgc ccgatgcgcg cacgcgggcc
    26461 gcggacacgc tgctgctggc ggtgaagccg caggacatgc gcgccgcact ggcaccactc
    26521 gcgggcgcgc tcgccggcaa gctggtcatc agcattgccg caggcatccg cctgcgcgag
    26581 ctttcgcgct ggctcggcgg gcacgccaag ctcgtgcgct gcatgccgaa tacgccggcg
    26641 ctgattggcg ccggcatcac cgggctctat gcgccgccgg gggtgacggc tgccgaaaag
    26701 cgccgcgccg agcgcatcct cggtgcggtt ggcgccaccg tctgggtcgg gaacgaggcg
    26761 ctgctcgacc cggtcaccgc ggtttccggc agcggcccgg cctatgtgtt ctggttcatc
    26821 gagcaactgg cggcggcggg cgaagccctc ggcctagcgc cgggcgtgtc gaggaagctg
    26881 gcactgcgca ccgtgctcgg cgcggcgcag ctcgcggcgg ggagccgcga gccgcccggc
    26941 gtgctgcgcg agcgcgtcac ctcgaaaggc ggtacgaccg cggcggcgct caaggtgttc
    27001 gaggacgaag ggctcggcga gcgcttccgg cgcgcggtcg cggccgccag ccggcgcggc
    27061 accgagatcg gcgacgagct ggggcgcgac tgatgctggt gcagatcggc cagttcctcg
    27121 tcgacgtcgt gggcagcttc ttcgtcttca tcctgctcgc gcgttttcac ttccagtggc
    27181 tgcgcgtgcc gttccgcaat ccgatcggcg aattcatgat cgcggtgacc aactggatcg
    27241 tgctgccggc gcggcgcgtg ataccgccgc tcgcggggct cgatctcgcg acactgatcg
    27301 ccgcctggct gctgcaggcg ctggcgctca cgctgctcgt ggccatggcg ggcggctcgg
    27361 gctcgacact ggtgttcgtc gcttcggcgt t
//
LOCUS       DBNHKE010000202         4855 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_301559, whole genome shotgun sequence.
ACCESSION   DBNHKE010000202 DBNHKE010000000
VERSION     DBNHKE010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_301559"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="lipA"
                     /locus_tag="AC16O9_13965"
     CDS             <1..831
                     /gene="lipA"
                     /locus_tag="AC16O9_13965"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR6517756.1"
                     /translation="GSPSTRFYEIKQILREHKLHTVCEEASCPNIGECFGKGTATFMI
                     LGDICTRRCPFCDVGHGRPLAPDAEEPAHLADTIAKLKLGYVVITSVDRDDLKDGGAQ
                     HFVDCIRAVREKSPRTTIEVLVPDFRGRLERALEVLLRAPPDVLNHNLETVPRLYRQA
                     RPGGDYRHSLKLLQEFAKAVPAVPTKSGLMVGLGETDEEILQVMRDLRTHEVQMLTIG
                     QYLQPSPHHLAVERFVHPEVFARFGREAAAMGFRHAAIGALVRSSYHADKQAEEAGVA
                     "
     gene            857..1294
                     /locus_tag="AC16O9_13970"
     CDS             857..1294
                     /locus_tag="AC16O9_13970"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HIR6517757.1"
                     /translation="MLVRALVLVAAASLAAAALAQKPPVFSDREGAIGGYDPVAFFTF
                     AAAIKGSPRITHQWRGATWHFAHSGNRDAFAADPEKYAPQYGGYCAYGVAQGYVVGID
                     PAAWTVVGDKLYFNYSPDVRRTWRQDVPGYVRKADANWPEVLR"
     gene            1333..2259
                     /locus_tag="AC16O9_13975"
     CDS             1333..2259
                     /locus_tag="AC16O9_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517758.1"
                     /translation="MPAIISIRGLTKTYASGLQALKPVDLEINKGEIFALLGPNGAGK
                     TTLISIVCGIVTPSTGTALVEGRDIVKDYRATRAMIGLVPQELHTDMFVTVWNTVRFS
                     RGLFGKAPNPQHLEKVLRDLSLWDKRDDRIMTLSGGMKRRLLIAKALSHEPEILFLDE
                     PTAGVDVALRRDMWELVRQLRASGVTIILTTHYIEEAEEMADRVGVISKGELIVVEDK
                     TALMKKLGKKQLTLHLQEPMAEIPAGLADWGLALKNGGCDLEYTFDARDERTGIPGLL
                     RRMSELGIGFKDLNTEQSSLEEIFVSLVSERA"
     gene            2256..3026
                     /locus_tag="AC16O9_13980"
     CDS             2256..3026
                     /locus_tag="AC16O9_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6517759.1"
                     /translation="MSAFNARGVLAIYLFEMSRFNRTFWQSLVAPVITTSLYFIVFGA
                     AIGSRMGELDGVHYGAFIVPGLIMLSIFTESLNNASFGIYLPRFTGTIYEVLSAPVSP
                     LEMVLGYVGAATTKSMILGFVILATATLFVPLKIVHPGWMVAFMVMTAATFCLFGFIL
                     GIWANGFEQLQFVPMLVITPLTFLGGAFYSIDMLPGAWRTFSLFNPVVYLISGFRWAF
                     YGTADVAVGVSLAATLGFFVACLAIVWWIFRTGYRLKN"
     gene            3125..4471
                     /locus_tag="AC16O9_13985"
     CDS             3125..4471
                     /locus_tag="AC16O9_13985"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6517760.1"
                     /translation="MAQANTPPEGSHFNVRVPARVLRAIEAQRARSEILTGWVQAIII
                     GVLATLYFVAPSTSPADIVFRPAPWAIGIYAAFTALRLRLAYAGRLTPSLRAASVIVD
                     MALLTVTIWGFHVEYGQPAAFYLKAPTFAYFFIFIALRTLSFSPAYVLLAGASAALGW
                     LALLLYALGEPGGMALVTHNYVEYMTAAKILVGGEVDKIVSLVLVSGLLALAVLRSRQ
                     LLEQAAAVQAAAAQMVRYFPPEVAEQLISADELLKPGQGEAREAAAMFIDLRGFTRLA
                     ETLTPRQLIGLVGEFQHLAVPIIQRHHGAVITYLGDGIMVTFGAVRPTQTYAADALRC
                     TEALIDGVGHWVAQSCARANCTPEVGIGVDVGTVICGTIGGEGKLEYAVLGEPVNRAA
                     KLQVHTKASGVRALTTRSALDKARAQGYDGARSSEVPGGARSVAGLDAPLNLVAIA"
     gene            complement(4528..4800)
                     /locus_tag="AC16O9_13990"
     CDS             complement(4528..4800)
                     /locus_tag="AC16O9_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517761.1"
                     /translation="MKRQVLAVALGTVFALPALANNEIDSGYTGAAQKSTPIAQVFVN
                     AEQGYVFAAGSSVDKSRAEVVAEVTKARSTGDYVANAELGIKASQL"
BASE COUNT          773 a         1596 c         1645 g          841 t
ORIGIN      
        1 ggctctccca gcacgcgctt ctacgagatc aagcaaatcc tgcgcgagca caagctgcac
       61 acggtgtgcg aggaggcctc gtgcccgaac atcggcgagt gcttcggcaa gggcaccgcg
      121 accttcatga tcctgggcga catctgcacg cggcgctgcc cattctgcga tgtcgggcac
      181 ggccggccgc tcgcgcccga tgccgaggag ccggcgcacc ttgccgatac gattgcgaag
      241 ctgaagctcg ggtacgtcgt catcaccagc gtcgatcggg acgacctgaa agacggcggc
      301 gcgcagcact tcgtcgactg catccgcgcc gtgcgggaga aaagcccgcg tacgacgatc
      361 gaggtgctgg tgccggattt ccgcgggcgg ctcgagcggg cgctggaagt gctgctccgc
      421 gcgccgcccg atgtcctgaa ccacaatctg gagaccgtgc cgcggctgta tcgccaggcg
      481 cgcccgggcg gcgactaccg gcattcgttg aagctgctgc aggaattcgc gaaggcggtg
      541 cccgcagtgc cgacgaaatc cggcttgatg gtgggtctcg gcgagaccga cgaggagatc
      601 ctgcaagtga tgcgcgacct gcgcacgcac gaggtgcaga tgctgaccat cgggcagtac
      661 ttgcagccct caccgcacca tctggccgtg gagcgcttcg ttcacccgga ggtctttgcg
      721 aggttcgggc gcgaggcggc agcgatgggt tttcgccatg cggcgatcgg ggcgctggtg
      781 cgctcgtcgt atcacgcgga caagcaggcg gaggaggcgg gcgtcgcata gatcttgcgc
      841 gggagggtgg tagcgcatgc tggtgcgtgc actggttctg gtggctgcgg cgagcctcgc
      901 cgcggccgcg ttggcgcaga agccgccggt attctcggat cgcgaaggtg cgatcggcgg
      961 ctacgacccg gtggccttct tcactttcgc cgcggcgatc aaaggttcgc cgcgcatcac
     1021 gcaccaatgg cgcggcgcca cctggcattt cgcccactcc gggaaccgcg acgcgttcgc
     1081 ggccgatccg gaaaagtacg cgccgcagta cggtggctac tgcgcctacg gcgtggcgca
     1141 aggctacgtg gtggggatcg atccggcggc gtggacggtg gtcggcgaca agctctactt
     1201 caattacagc ccggacgtca ggcgaacctg gcggcaggac gtgccgggct acgtgcgcaa
     1261 ggcggatgcg aactggcccg aggtcctgag gtaggcggcg ttcaggcgag catgaagtag
     1321 tatctcggcc ccatgccggc gatcatctcc atccgcggtc tcaccaagac ctatgcctcg
     1381 ggcctgcagg cgctaaagcc ggtcgatctc gagatcaaca agggcgagat cttcgcgctc
     1441 ctcggcccga acggcgccgg caagaccacg ctgatcagca tcgtctgcgg catcgtcacg
     1501 ccgagcacgg gaacggcgct cgtcgaaggc cgggacatcg tcaaggacta ccgcgccacg
     1561 cgcgcgatga tcggcctcgt gccgcaggag ctgcacaccg acatgttcgt caccgtctgg
     1621 aacacggtgc gtttcagccg cgggctgttc ggaaaagcgc ccaacccgca gcacctggag
     1681 aaggtgctgc gcgacctgtc gctgtgggac aagcgcgacg atcgcatcat gacgctttct
     1741 ggcggcatga agcggcgcct gctcatcgcc aaggcgctgt cgcacgagcc tgaaatactg
     1801 ttcctcgacg agcccaccgc gggggtggac gtggcgctgc ggcgcgacat gtgggagctg
     1861 gtgcgtcagc tgcgcgcgag cggcgtcacc atcatcctca ccacgcatta catcgaggaa
     1921 gccgaagaaa tggccgatcg cgtcggcgtc atcagcaagg gcgagctgat cgtggtcgag
     1981 gacaagaccg cgctgatgaa gaagctgggc aagaagcagc tcacgctgca cctgcaggag
     2041 cccatggccg agattccggc gggtctcgcg gactgggggc tcgccctgaa gaacggcggc
     2101 tgcgacctcg aatacacttt cgatgcgcgc gacgagcgca ccggcatccc cggcctcctg
     2161 cggcgcatga gcgagctcgg catcggcttc aaggatctga acaccgagca gagctcgctg
     2221 gaagagatct tcgtgagcct ggtgagcgag cgcgcatgag cgccttcaac gcccgcggcg
     2281 tcctcgccat ctacctgttc gagatgtcgc gcttcaaccg caccttctgg cagagcctgg
     2341 tggcgcccgt gatcaccacg tcgctctact tcatcgtctt cggcgcggcc atcggctcga
     2401 ggatgggcga gctggacggc gtgcactacg gcgcgttcat cgtgccgggg ctgatcatgc
     2461 tctccatctt caccgagagc ctgaacaacg cctccttcgg catctacctg ccgcgcttca
     2521 ccggcaccat ctacgaggtg ctctcggcgc cggtctcgcc gctcgagatg gtgctcggct
     2581 acgtcggcgc ggccacgacc aagtccatga tcctggggtt cgtcatcctc gccacggcga
     2641 cgctgttcgt gcccctgaag atcgtgcatc cgggctggat ggtggctttc atggtgatga
     2701 cggcggccac gttctgcctg ttcggcttca tcctcggcat ctgggcgaac ggcttcgagc
     2761 agctgcagtt cgtgccgatg ctggtcatca ccccactcac gttcctcggt ggcgcgttct
     2821 actccatcga catgctgccc ggggcctggc gcacgttcag cctcttcaac ccggtcgtgt
     2881 acctgatcag cggctttcgc tgggcgttct acggcacggc ggacgtggcg gtaggggtga
     2941 gcctcgcggc gacgctcggc ttcttcgtcg cctgcctggc gatcgtctgg tggatcttca
     3001 ggaccggata ccggctgaag aactgagcgg aagcgcgcgt taccgcgtgg cgtaagcgaa
     3061 tgtcgcgccc gtaaggtcgg cggtgctggc gcgacccatg taggtcacat cggaatacaa
     3121 cgccatggct caggcgaata cgcctccgga gggttcccac ttcaatgtgc gtgtccccgc
     3181 gcgcgtgctg cgcgccatcg aggcgcagcg cgcgcgcagc gagatcctca ccggctgggt
     3241 acaggcgatc atcatcggcg tgctggcgac gctgtacttc gtcgccccaa gcacgtcgcc
     3301 tgccgacatc gtgttccgcc cggcgccctg ggcgatcggc atctacgccg cattcaccgc
     3361 gctgcggctg cggctggcct acgccggccg gctgacgccg tcgctgcgcg ccgcctccgt
     3421 gatcgtcgac atggcgctgc tcacggtgac catctggggc tttcacgtcg agtacgggca
     3481 acccgccgcc ttctatctca aggcgccgac cttcgcctac ttcttcatct tcatcgccct
     3541 gcgcacgttg tctttctcgc cggcctacgt gctgctcgcc ggcgcaagcg cggcactggg
     3601 ctggctcgcg ctgctgctct acgcgctcgg cgagccgggc ggcatggcgc tcgtcacgca
     3661 caactacgtc gagtacatga cggcggcgaa gatcctggtc ggcggggaag tcgacaagat
     3721 cgtgtcgctg gtgctggtat ccggactcct cgcgctcgcc gtcttgcgta gccgccaact
     3781 gctcgagcag gcggcggcgg tccaggcggc ggccgcgcag atggtgcgct atttcccgcc
     3841 tgaagtggcc gagcagctca tcagcgcaga cgagctcctg aaaccggggc agggcgaggc
     3901 gcgcgaggcg gcggcgatgt tcatcgacct gcgcggcttc acgcgccttg ccgagaccct
     3961 cacgcccagg cagctcatcg ggctggtggg cgagttccag cacctggcgg tgccgatcat
     4021 ccagcgccat cacggcgccg tcatcaccta cctcggcgac ggcatcatgg tgacctttgg
     4081 cgcggtacgt cccacgcaga cctacgccgc cgatgcgctg cgctgcacgg aagccctgat
     4141 cgatggcgtc gggcattggg tagcgcaaag ttgcgcccgc gccaattgca ccccggaggt
     4201 gggcatcggc gtggacgtcg gcacggtgat ctgcggcacc attggcggcg agggcaagct
     4261 cgaatacgcg gtgctgggcg agcccgtgaa ccgcgcggcc aagctgcagg tgcacaccaa
     4321 ggcgtcgggg gtgcgcgcgc tcacgacgcg cagtgcgctc gacaaggcgc gcgcccaggg
     4381 ctacgacggc gcgcgttcga gcgaggtccc ggggggcgcg cgaagcgtgg cgggtctcga
     4441 cgcaccgctc aacctggtgg ccatcgcctg aaaagaaaaa gcgccgcggc ttgccgcggc
     4501 gctgcccggt tgacctgctt gcggggctta cagctggctg gctttgatgc cgagctcggc
     4561 gttggcgacg tagtcgcccg tgctgcgcgc cttcgtgact tcggccacga cttcagcgcg
     4621 cgacttgtcg acgctcgagc cggccgcaaa cacgtagccc tgctcggcat tgacgaacac
     4681 ctgcgcgatc ggcgtggatt tctgcgccgc gccggtgtag ccggaatcga tttcgttgtt
     4741 ggcgagggcc ggcagcgcaa acacggtgcc gagggcgact gcgagaactt ggcgtttcat
     4801 ggtgaggctc ctttcaagtg gttaatcgtc cgctccggca ccgccgaagc catga
//
LOCUS       DBNHKE010000203         4000 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_882396, whole genome shotgun sequence.
ACCESSION   DBNHKE010000203 DBNHKE010000000
VERSION     DBNHKE010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_882396"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC16O9_13995"
     CDS             <1..971
                     /locus_tag="AC16O9_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR6517762.1"
                     /translation="RDAIERAGARRAMAERAAEHLVLAELHGLPTHGMSRVPFYCAML
                     KNGRADGAARPALVAEKAAVCLIDNADGLPYESCAWAIEEVIQRARRNGIGFAGIRNS
                     AHVGVLGIHLLKVAAAGLVGVAFTNSPAAIPAWGGKKALFGTDPVAFAFPRQDADPIV
                     VDLALTTVVRGKIMVAMQKGERIPEGWALDRDGKPTTDPKEAIERGSLFPIGGAKGAM
                     LALAFELVCAALTGAAIGPEADSFFAEQGNRPRIGQAFLAIDPAALAGTDKYLERVEA
                     VVRAMLADEGVRLPGSKRFAAETRFAKEGIEVPDDLLAKIEKLCST"
     gene            959..1441
                     /locus_tag="AC16O9_14000"
     CDS             959..1441
                     /locus_tag="AC16O9_14000"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6517763.1"
                     /translation="MFDVVLAFPKMKVAVLTRRDLVAEIRYLPPAAETLAPQNALAER
                     AARQLERYRDDPDAPFDLPLAIEGTPFQKRVWEALCAIPRGRTRSYGELARELGGEAR
                     AVGQACGDNRLPIAIPCHRVVAADGVGGFAHATDGYLIEAKRWLLLHEAQADAFALKP
                     "
     gene            1390..2319
                     /gene="xerD"
                     /locus_tag="AC16O9_14005"
     CDS             1390..2319
                     /gene="xerD"
                     /locus_tag="AC16O9_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR6517764.1"
                     /translation="MAAPARSASGRLRAQALSAADAARVDAFCDALWLEDGLARNTLE
                     AYRRDLAQLGAFLKGRALLQAREEDLFGFLAARRGKNTSAARMLSTLKRFYRHCLREH
                     QIRADPTLKLDPPRRAPRFPKSLSEADVEALLAAPDADTPRGLRDRAMLEALYATGLR
                     VSELVALKGFEANLDAGVVRVLGKGSKERLVPLGEEAVSWLQRYLARARPALLKRRSS
                     DALFVTDRGAGMTRQAFWHLIKRYGRRAIPGKALSPHVLRHAFATHLINHGADLRVVQ
                     MLLGHADISTTQIYTHVARERLKALHAKHHPRG"
     gene            2312..2785
                     /gene="ybaK"
                     /locus_tag="AC16O9_14010"
     CDS             2312..2785
                     /gene="ybaK"
                     /locus_tag="AC16O9_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR6517765.1"
                     /translation="MADTPATVFLKARGVAYTEHEYDYVEHGGTEVSARELGVAEHDV
                     VKTLVMQDESGTPLLVLMHGDRTVSTKNLARQAGVKRIEPCRPEVAQRHSGYQIGGTS
                     PFGTKKPLRVYLERSVLELPKVYINGGRRGFLVGLAPGEIVRVLQPVLVEVGLAD"
     gene            complement(2782..3132)
                     /locus_tag="AC16O9_14015"
     CDS             complement(2782..3132)
                     /locus_tag="AC16O9_14015"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6517766.1"
                     /translation="MDAIVIRDLRVEALIGIHQRERHVAQTVSVDLEIGLPGAAVFKS
                     DKVSDTIDYEQVALKIRALAGSGHFRLVETLAERIARLLLEDFGAPWVKVGAAKVGIL
                     ANAKVVGVTIERKR"
     gene            3173..3811
                     /gene="plsY"
                     /locus_tag="AC16O9_14020"
     CDS             3173..3811
                     /gene="plsY"
                     /locus_tag="AC16O9_14020"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR6517767.1"
                     /translation="MQTALVVLGAYLIGSISFAIALSRLAGMPDPRSYGSGNPGATNV
                     LRTGRKVVAALTLAGDGAKGWLAVFLAQWWTGQSAIGGLDVPLAGVAVFLGHLFPVFH
                     GFKGGKGVATAAGVLLAFNAWLGLATLATWAAIFAFFRISSLAGLVAAAFAPLYTFWL
                     FGLRPVFPAVIAIAVLMAWRHKDNFRRLLSGAESRVGADPSPPPQDPSAGAP"
     gene            complement(3771..>4000)
                     /locus_tag="AC16O9_14025"
     CDS             complement(3771..>4000)
                     /locus_tag="AC16O9_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041949853.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6517768.1"
                     /translation="LVVAGGVGANRRLRSALDARAKVQGFDVVYPEPELCTDNGAMIA
                     LAAALRLQAGLADGATHAFTVRPRWDLAAAG"
BASE COUNT          558 a         1420 c         1422 g          600 t
ORIGIN      
        1 cgcgggacgc gatcgagcgc gcaggcgcgc ggcgcgcgat ggccgagcgc gcggcggagc
       61 atctcgtgct cgccgaactg catgggctgc ccacgcacgg catgtcgcgc gtgccctttt
      121 attgcgcgat gctgaagaac ggccgcgccg acggcgcggc gcgcccggcg ctcgttgcgg
      181 agaaggcggc ggtgtgcctg atcgacaacg ccgatggcct gccgtacgag tcctgcgcct
      241 gggcgatcga ggaggtgatc cagcgcgcgc gccgcaacgg catcggcttt gccggcatcc
      301 gcaacagcgc gcacgtcggc gtgctcggca ttcacttgct gaaggttgcg gccgcgggtc
      361 tggtcggcgt ggcgttcacc aactcgcccg cggcgattcc cgcctggggc ggcaagaagg
      421 cgctgttcgg caccgacccc gtcgccttcg cctttccgcg ccaggacgcc gatccgatcg
      481 tcgtcgacct cgcgctcacc accgtggtgc gcggcaagat catggtggcg atgcaaaagg
      541 gcgagcgcat tcccgagggc tgggcgctcg atcgcgacgg caagcccacc accgacccga
      601 aggaggcgat cgagcgcggc agcctcttcc cgatcggcgg cgcgaagggc gcgatgctcg
      661 cgctcgcctt cgagctggtg tgcgccgcgc tgaccggtgc ggcgatcggt cccgaggccg
      721 actcgttctt cgccgagcag ggcaacaggc cgaggatcgg ccaggcgttc cttgccatcg
      781 atccggcggc gctcgccggc acggacaagt atctcgagcg cgtggaggcg gtggtgcgcg
      841 cgatgctcgc cgacgagggg gtgcggcttc cgggatcgaa gcgctttgcc gccgagacac
      901 gtttcgcgaa ggagggcatc gaggtgcccg atgatcttct ggcgaagatc gagaaactat
      961 gttcgacgta gttctcgcct ttccgaagat gaaggttgcg gtgctgacgc gccgcgacct
     1021 ggttgccgag atccgctatt tgccgcctgc ggccgagacg ctcgcgccgc aaaacgcgct
     1081 cgccgagcgc gcggcacgcc agctcgagcg ctaccgcgac gatcccgatg cgccgttcga
     1141 cctgccgctc gcgatcgagg gtacgccatt ccagaagcgc gtctgggagg cgctgtgcgc
     1201 gatcccgcgc ggccgcacgc gcagctacgg ggagctcgcg cgcgagctcg gcggcgaggc
     1261 gcgcgccgtc ggccaggcct gcggcgacaa ccgcctgccg atcgcgattc cctgccaccg
     1321 tgtcgtggcc gccgacggcg tcggcggctt cgcccacgcc accgacggct acctgatcga
     1381 ggccaagcgt tggctgctcc tgcacgaagc gcaagcggac gccttcgcgc tcaagccctg
     1441 agcgcggccg acgccgcccg ggtggacgcg ttctgcgacg cgctctggct ggaggacggg
     1501 ctggcgagga acacgcttga agcctatcga cgcgacctgg cgcagctcgg cgcgttcctg
     1561 aaggggcgcg cgctgctgca ggcgcgcgaa gaagatctgt tcggcttcct cgccgcgcgc
     1621 cgcggcaaga acacgagcgc ggcccgcatg ctctcgacgc tgaagcgctt ctaccggcac
     1681 tgcctgcgcg agcaccagat ccgggccgac ccgacgctga agctcgatcc gccgcggcgc
     1741 gcgccgcgct tcccgaagag cctgtcggag gccgacgtgg aggcgctgct cgccgcgccg
     1801 gacgctgaca cgccgcgcgg cctgcgcgac cgcgccatgc tggaggcgct ctacgcgacc
     1861 gggctgcgcg tgtccgagct ggtggcgctg aagggcttcg aggcgaacct cgacgcgggc
     1921 gtggtgcgcg tgctcggcaa gggctcgaag gagcgcctgg tgccgctcgg cgaggaggcg
     1981 gtgtcctggc tgcagcgcta cctcgcgcgc gcgcgtccgg ccctgctcaa gcgcaggtcg
     2041 agcgacgcgc tgttcgtcac cgatcgcggc gcgggcatga cgcggcaggc cttctggcac
     2101 ctcatcaagc gctatggccg gcgcgcgatc cccggcaagg cgctctcacc gcacgtgctg
     2161 cgccacgcct tcgccacgca cctcatcaac cacggcgcgg acctgcgcgt ggtgcagatg
     2221 ctgctcggcc acgccgacat ctccaccacg cagatctaca cccacgtcgc gcgcgagcgc
     2281 ctgaaggcgc tgcacgcgaa gcaccacccg cgtggctgat acgccggcca cggtgttcct
     2341 gaaggcgagg ggggtcgcct acaccgagca cgagtacgac tacgtcgagc acggcggaac
     2401 ggaagtgtcc gcgcgcgagc tgggcgtggc cgagcacgac gtggtgaaga cgctggtgat
     2461 gcaggacgag tcgggaacgc ccctgctcgt gctcatgcac ggcgaccgca cggtctccac
     2521 caagaacctc gcgcgccagg cgggcgtgaa gcgcatcgag ccgtgccggc ccgaagtcgc
     2581 gcagcgccat tcgggctacc agatcggcgg cacctcgccc ttcggcacga aaaagccgct
     2641 gcgcgtgtac ctcgagcgct cggtgctcga gctgccgaag gtctacatca acggcggccg
     2701 gcgcggattc ctcgtcgggc ttgcgcccgg ggagatcgtg cgcgtgctgc agccggtgct
     2761 ggtcgaggtg gggctggcgg actagcgctt gcgctcgatc gtgacgccga cgaccttcgc
     2821 gttggcgagg atgccgacct tcgccgcgcc gactttcacc cacggcgcgc cgaagtcctc
     2881 gagcagcagc cgcgcaatgc gctcggcgag cgtctcgacc agccggaaat gcccgctccc
     2941 ggcgagcgcg cggatcttca gcgccacctg ctcgtagtcg atggtgtcgg acaccttgtc
     3001 gctcttgaac acggcggcgc cggggaggcc gatttccagg tcgacggaca cggtctgcgc
     3061 cacgtgccgc tcgcgctggt ggatgccgat gagcgcttcg acgcgcaggt cgcggatcac
     3121 gatggcgtcc atggtggcgc gattataggg gcgcgcgggt aacattcggc ccatgcagac
     3181 cgcgctcgtc gtgctcgggg cctacctgat cggctcgata tccttcgcca tcgcgctgtc
     3241 gcgcctggcg ggcatgcccg acccgcgcag ctacggctcg ggcaatcccg gggccaccaa
     3301 cgtgctgcgt accggccgga aggtcgtcgc ggcgctcacc ctcgccggcg acggggccaa
     3361 gggctggctg gcggtgttcc tggcccaatg gtggaccggc cagtcggcca tcggcgggct
     3421 ggacgtgccg ctcgccggcg tcgccgtatt cctcggccat ctgttcccgg tgtttcacgg
     3481 cttcaagggg ggtaagggcg tggcgaccgc cgccggcgtg ctcctagcgt tcaacgcatg
     3541 gctcggcctc gccaccctcg cgacctgggc ggcgatcttc gctttcttcc gcatctcgtc
     3601 gctcgcggga ttggtggccg cggcgtttgc gccgctctac accttctggc tgttcgggct
     3661 gcggccggta ttccctgcgg tcatcgccat cgccgtgctc atggcctggc ggcacaagga
     3721 caatttccgg cgcctgctgt ccggcgccga aagccgcgtc ggcgcagacc ctagcccgcc
     3781 gccgcaagat cccagcgcgg gcgcaccgta aaggcgtgcg tcgcgccgtc ggcgaggccc
     3841 gcctgcaggc gcagcgcagc cgccagcgcg atcatggcgc cgttgtcggt gcacagctcg
     3901 ggctccggat acaccacgtc gaagccctgc accttcgccc gggcatcgag cgcgctgcgc
     3961 agccgccggt tggcgccgac gccgccggcg accaccagct
//
LOCUS       DBNHKE010000204         4740 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_830491, whole genome shotgun sequence.
ACCESSION   DBNHKE010000204 DBNHKE010000000
VERSION     DBNHKE010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_830491"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="AC16O9_14030"
     CDS             <1..115
                     /locus_tag="AC16O9_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HIR6517769.1"
                     /translation="RPETWIQWLAVVAIGLTAINMFGGFAVTQRMLQMFRK"
     gene            128..1543
                     /gene="pntB"
                     /locus_tag="AC16O9_14035"
     CDS             128..1543
                     /gene="pntB"
                     /locus_tag="AC16O9_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HIR6517770.1"
                     /translation="MSATLATVSYIGATILFILSLGGPSHPETSRRGNLYGIIGMAIA
                     VLATVFGPRVTPAGYAWIIIAMLIGGSVGLYASRIVRMTQMPELVALMHSLVGLAAVL
                     VGFANFIDPAASVGFSGAEKTIHEVEIYLGILIGAVTFSGSVIAFGKLSAKISGKPML
                     LPGRHWLNLAGLAVVLWFGWRFIQAHSIADGMLPLVVMTVIALLFGIHMVMAIGGADM
                     PVVVSMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRRFLAVIA
                     GGFGTEGGAVAAKGGAQPAGEVAPISAPETAELLREAKNVIIVPGYGMAVAQAQHTVY
                     EITRHLREKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVQEMDELNDDFPQTDVA
                     MVIGANDIVNPAAQEDPASPIAGMPVLEVWKAKTSVVMKRSMASGYAGVDNPLFYKEN
                     NRMLFGDAKKMLDEVLVALKG"
     gene            1602..2438
                     /locus_tag="AC16O9_14040"
     CDS             1602..2438
                     /locus_tag="AC16O9_14040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6517771.1"
                     /translation="MQRSLVARLRALTDRLGRRMSAPTLKIGVSARILHPQRGARGLQ
                     SKTLQYLEQTAAHWLMSRDVLVLMVPTVDTGGILHRSSIRLSDYARHLDGLMLQGGAD
                     VSPLSYGEDPLSREWAGDRLRDEYEIELLHEFIEAGKPVLGVCRGLQLINVAYGGTLY
                     QDIVQQVPKARRHVTEDYEKNLHEVHFEPAGALARLYPGVERARVNSIHHQSVKTLGR
                     NLRVEAWSESDRVVEAIRGTGASYVLGVQWHPEFHPPGDQSVLDSAPILDEFLRAAQR
                     AA"
     gene            2444..3655
                     /locus_tag="AC16O9_14045"
     CDS             2444..3655
                     /locus_tag="AC16O9_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6517772.1"
                     /translation="MGLGFLPELPPAFGQPALFGALLLAGLLGGELARRLLGLPRITG
                     YVLAGVLLGPQASGLLAPGALGEARVLIDLSLGLIVFELGHRLDVQWLHRNRWLFVAA
                     IAESAFCFGAVLGTLLAFGFQPLLAASAAAIGTATSPAVVLLVAHELRASGQVTERML
                     LFTAVNCVLAYIALTLLLPFLHLEHAVSWREAVLHPLYILGGSALVGSLASLSLLWLA
                     GWVGKREDRQFILLIALVVLAVGAARSLNLSVVVTLIILGMLARNFDYEQVLLPLRFG
                     YGGQLFFVILFVLTGASVEFTALPGVAAAVLAFIVARFAGKGLAILVFGRLSGVGPRS
                     AGLLSLALLPMSGLAVVMVLDTQALYPAFGGELAAVVLTAVAVLEILGPIATQFALRR
                     AGEADPAAGSA"
     gene            3664..>4740
                     /locus_tag="AC16O9_14050"
     CDS             3664..>4740
                     /locus_tag="AC16O9_14050"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HIR6517773.1"
                     /translation="MDETDFKASPELTFGVELELQLVQPHDLDLARDAGELLARLEKL
                     KLPGAVKPEITESMIELNTSIHRSYPSLAAELDAMRDAVVKEAGMLNLRVCGGGSHPF
                     HSWSERRVFPTERFRHILERYGYLAKQFTVFGQHIHVGCASGDDAVYLVHMMTRYVPH
                     FIALSAASPFYQGEDTSFQSSRLTAINAFPLAGHMPFVDDWAEFRVYFEKLRALGVVE
                     SMKDFYWDIRPKPEYGTVEIRVCDTPLSVARAAQLAAYAQALAKWLLEQRPRQPKRAV
                     YLVNAFNRFEAARFGLRGQLIDPYEEVKRPLAEDILDALAGVMPQAAALESADALSAL
                     AADVRRGYSDAGWLRERFAANQSRG"
BASE COUNT          656 a         1655 c         1632 g          797 t
ORIGIN      
        1 ccggccggag acctggatcc agtggctcgc ggtggtcgcc atcggcctta ccgcgatcaa
       61 catgttcggc ggcttcgccg tgacgcagcg catgctgcag atgttccgca agtaagggag
      121 acgatccatg tccgcgactc tggcgacggt ctcttacatc ggcgcgacca tcctgttcat
      181 cctcagcctc ggcggcccct cgcacccgga gacctcgcgg cgaggcaacc tctacggcat
      241 catcggcatg gcgatcgccg tgctggcgac ggtgttcggg ccgcgcgtca cgcccgccgg
      301 ctacgcctgg atcatcatcg ccatgctgat cggcggcagc gtcggcctgt acgcctcgcg
      361 catcgtgagg atgacgcaga tgcccgagct ggtggcgctg atgcacagcc tggtcggcct
      421 cgctgcggtg ctggtgggtt tcgccaactt catcgacccg gcggcctcgg tgggcttcag
      481 cggcgcggag aagaccatcc acgaggtgga gatctacctc gggatcctga tcggcgcggt
      541 gaccttctcg gggtcggtga tcgcgttcgg caagctctcg gccaagatca gcggcaagcc
      601 gatgctgctg cccgggcgcc actggctgaa tctcgcgggc ctcgcggtgg tgctctggtt
      661 cggctggcgc ttcatccagg cgcactcgat cgccgacggc atgctgccgc tggtggtgat
      721 gaccgtgatc gcgctgctgt tcggcatcca catggtgatg gcgatcggcg gcgccgacat
      781 gccggtggtg gtatcgatgc tgaacagcta ctccggctgg gcggccgcgg ccacgggctt
      841 catgctgtcc aacgacctgc tgatcgtcac cggcgccctg gtcggctctt cgggggcgat
      901 cctgtcctac atcatgtgcc gcgcgatgaa ccggcgcttt ttggcggtga tcgccggcgg
      961 cttcggcacc gagggcgggg ccgtggctgc caagggcggc gcgcagcccg cgggcgaggt
     1021 cgcgccgatc agcgcgcccg agaccgccga gctgctgcgc gaggcgaaga acgtgatcat
     1081 cgtcccgggc tacggcatgg cggtggcgca ggcgcagcac acggtctacg agatcacccg
     1141 gcacctgcgc gaaaagggcg tcaacgtgcg ctttggcatc cacccggtgg ccgggcgcat
     1201 gccgggccac atgaacgtcc tgctcgccga ggccaaggtg ccctacgaca tcgtgcagga
     1261 gatggacgag ctgaacgacg actttccgca gaccgacgtc gccatggtga tcggcgcgaa
     1321 cgacatcgtg aatccggccg cccaggagga ccccgcgagc ccgatcgccg gcatgcccgt
     1381 gctcgaggtt tggaaggcga agacctcggt ggtgatgaag cgcagcatgg cctcgggtta
     1441 tgccggcgtg gataacccgc tcttctacaa ggagaacaac cgcatgctgt tcggcgacgc
     1501 gaagaagatg ctcgacgagg ttctggttgc cctgaagggc tgatcgaaac tccggccgcg
     1561 gtccgcagtc agaccgcgaa taccaacccg ggagagaccc cttgcagcgc tcgctcgtcg
     1621 cccgcctgcg cgccctgacc gaccggctcg gccgccgcat gagtgcgccg acgctgaaaa
     1681 tcggcgtctc ggcgcgcatc ctgcatccac agcgcggcgc gcgcggcctg cagtcgaaga
     1741 cgctgcagta cctcgagcag accgccgcgc actggctcat gtcgcgcgac gtgctggtgc
     1801 tgatggtgcc caccgtggat accggcggca tcctccaccg cagcagcatc cgcctgtcag
     1861 actacgcgcg ccacctcgac ggcctcatgc tgcagggcgg cgccgacgtc agccccttga
     1921 gctacggcga ggatccgctg tcgcgcgagt gggcggggga ccgtctgcgc gacgaatacg
     1981 agatcgagtt gctgcacgag ttcatcgagg cgggcaagcc ggtgctcggc gtgtgccgcg
     2041 gcctgcagct catcaacgtc gcctacggcg gcacgctcta ccaggacatc gtgcagcagg
     2101 tgcccaaggc gcggcgccac gttaccgagg actacgagaa gaatctgcac gaggtgcatt
     2161 tcgagccggc gggggcgctc gcccgcctgt acccgggcgt cgagcgcgcg cgcgtcaact
     2221 ctatccacca ccagtccgtg aagaccctgg gccgcaacct gcgcgtcgag gcctggtccg
     2281 aatccgaccg cgtcgtcgag gcgatccgcg gcaccggcgc gagctacgtg ctcggcgtgc
     2341 aatggcaccc cgagttccat cccccgggcg accagagcgt gctcgacagc gcgccgatcc
     2401 tcgacgaatt cctgcgcgcg gcgcagcgcg cggcctgagc ccgatgggcc tcggctttct
     2461 gcccgaactg ccgcccgcct tcggccagcc ggcgctgttt ggcgcgctgc tgctcgccgg
     2521 gctgctgggc ggcgagctgg cgcggcggct gctcgggctg ccgcgcatca cggggtacgt
     2581 gctcgccggg gtgctgctcg gaccccaggc ctcggggctg ctcgcccccg gcgcgctcgg
     2641 cgaggcgcgc gtgctgatcg acctctcgct cgggctgatc gtgttcgagc tcggccaccg
     2701 cctcgacgtg cagtggttgc accgcaaccg ttggctgttc gtcgccgcta tcgccgagag
     2761 cgcattctgc ttcggcgcgg tgctcgggac gctcctcgcc ttcggtttcc agccgctgct
     2821 cgcggcatcc gccgcggcca tcggcaccgc gacgtcgccc gcggtggtgc tgctggtggc
     2881 gcacgagctg cgcgcctcgg gccaggtgac cgagaggatg ctcctgttca ccgccgtgaa
     2941 ttgcgtgctc gcctacatcg cgctgacgct gctgctgccg ttcctgcatc tcgagcacgc
     3001 ggtaagctgg cgcgaagccg tgctgcaccc cctctacatc ctcggcggct cggcgctggt
     3061 cggctccctt gccagcctgt cgctgctgtg gctcgccggc tgggtcggca agcgcgagga
     3121 ccgccagttc atcctgctca tcgcgctggt ggtgctggcg gtgggcgccg cgcgcagcct
     3181 caacctgtcg gtggtggtga ccctcatcat cctcggcatg ctggcgcgca acttcgacta
     3241 cgagcaggtg ctgctgccgc tgcgcttcgg ctacggcggg cagctgttct tcgtcatcct
     3301 gttcgtgctg accggcgcca gcgtcgagtt cacggcactg ccgggcgtgg ccgcggccgt
     3361 gctcgccttc atcgtcgcgc gctttgccgg caagggcctc gccatcctcg tcttcgggcg
     3421 gctctcgggg gtgggtccgc gcagcgccgg gctgctctcg cttgcgctgc tgccgatgtc
     3481 gggtctggcc gtggtgatgg tgctggacac gcaggccctg tacccggcgt tcggcggcga
     3541 actggcggcg gtggtgctca cggcggtggc ggtgctcgag atcctcgggc ccatcgccac
     3601 ccagttcgcg ctgcgccgcg ccggcgaggc cgacccggcg gccggcagcg cctgagggcc
     3661 gacgtggacg agaccgattt caaggcctcg ccggagctca ccttcggcgt cgagctcgag
     3721 ttgcagctgg tgcagccgca cgaccttgac ctggcgcgcg acgccggcga gctgctggcg
     3781 cgactcgaga agctgaagct ccccggggcg gtgaagcccg agatcaccga gagcatgatc
     3841 gagctgaaca cctcgatcca ccgcagctat cccagcctgg ccgcggagct cgacgccatg
     3901 cgcgatgcgg tggtgaagga agccggcatg ctgaatcttc gcgtgtgcgg cggcggctct
     3961 cacccgttcc acagctggag cgagcggcgc gtgttcccca ccgagcgctt ccgccacatc
     4021 ctcgagcgct acggctacct cgccaagcag ttcaccgtgt tcggccagca catccacgtc
     4081 ggctgcgcga gcggcgacga cgcggtgtac ctggtgcaca tgatgacgcg ctacgtgccg
     4141 catttcatcg ccctgtcggc cgcttcgccc ttctaccagg gcgaagacac gtcgttccag
     4201 tcctcgcgtc tcaccgcgat taacgccttc ccgctggccg ggcatatgcc gtttgtcgat
     4261 gactgggcgg agttccgcgt gtacttcgag aagttgcgcg cgctcggcgt ggtggagagc
     4321 atgaaggact tctactggga catccgcccc aagcccgagt acggcaccgt cgagatccgc
     4381 gtctgcgaca cgccgctcag cgtggcgcgc gcggcgcagc tcgccgccta cgcgcaagcg
     4441 ctcgccaagt ggcttctcga gcagcgcccg cgacagccca agcgcgccgt gtacctggtg
     4501 aacgccttca accgcttcga ggcggcgcgc ttcgggctgc gcggccagct catcgacccg
     4561 tacgaggaag tgaagcgccc gctcgccgag gacatccttg acgcgctggc gggcgtcatg
     4621 ccgcaggcgg ctgcactgga aagcgccgac gcgctgtcgg cgctcgccgc cgacgtgcgc
     4681 cgcggctatt cggacgccgg ctggctgcgc gagcgcttcg ccgccaacca gtcgcggggg
//
LOCUS       DBNHKE010000205        10853 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_513718, whole genome shotgun sequence.
ACCESSION   DBNHKE010000205 DBNHKE010000000
VERSION     DBNHKE010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_513718"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="AC16O9_14055"
     CDS             <1..904
                     /locus_tag="AC16O9_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6517774.1"
                     /translation="GTPNTKVKNTQVHGAEVVLEGDTLSDSGAHARELAAREGYTFVH
                     PYDDAAVVAGQGTVGLEMLEDVPGLEMLVVPVGGGGLASGIATAAKALRPETHVYGVE
                     SKAWSAMFQRMHGLPVDVGGDTIAEGIAVRDVSALTLEICKRFLDEVLLVDEEIIERA
                     IVALIEIEKTVAEGAGAAGLAAVLAHPQRFAGRRVGIPICGGNIDSRVLSAVLMRGLV
                     RDGRLVRMRVMMPDLSGSLAKVAALIAAAGGNIVEVQHQRVFGTASVRTPEVEFVIET
                     RDREHTRAIVAHLSTHGATARLDT"
     gene            complement(901..1290)
                     /locus_tag="AC16O9_14060"
     CDS             complement(901..1290)
                     /locus_tag="AC16O9_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6517775.1"
                     /translation="MIDHVDHIVLTTRDLEGCVRFYRDVLGMALESFRTPTETRQALK
                     FGNQKINLHEWGKEFVPRAHVAAPGTLDLCFIASVPLEQVIARLNAANVKILEGPVMK
                     TGAVSKIRSVYVRDPDLNLVEISEPVG"
     gene            complement(1358..1813)
                     /locus_tag="AC16O9_14065"
     CDS             complement(1358..1813)
                     /locus_tag="AC16O9_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6517776.1"
                     /translation="MSDNVHPIGRGYCYEDLKVGMRFRTHRRTIAEADLAGFVNLTWL
                     TEELFAVEDDSQRAIKGRPVPGALVYSFAEGLLLPSMQDTGLAFLNATLDVKGPTVVG
                     DTIHVEVEVTEARLTSKGDRGLVRFANKVVNQRGETVIEYNPLRMLKRK"
     gene            complement(1815..2591)
                     /locus_tag="AC16O9_14070"
     CDS             complement(1815..2591)
                     /locus_tag="AC16O9_14070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517777.1"
                     /translation="MSAFDLKGKVALVTGGNGGIGLGMAEGLAAAGADVVIWGSNPDK
                     NAAAEKLLKKHGRRVLAQRCDVSDEAQVERSFAEALKAMGRVDGCFANAGVSQVRAPL
                     AELPTKEWRRVMQVNLDGAFFTLRAAARHMTGRAVAGDAGGRLVGMASSAAIHGAARA
                     SSYAATKGAMLTMIRALAVEMARFQVTANSILPGWIESDMTAAAIGDAKFSAAVLPRI
                     PLRRWGKKEDFAGIAVYLMSDASGYHTGDSFVIDGAYTIF"
     gene            complement(2588..2920)
                     /locus_tag="AC16O9_14075"
     CDS             complement(2588..2920)
                     /locus_tag="AC16O9_14075"
                     /inference="COORDINATES: protein motif:HMM:NF052504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sll1863 family stress response protein"
                     /protein_id="HIR6517778.1"
                     /translation="MAKIEAPSTKEVVMMNRDEYVRKLKAQLDQWNAEAKQWEAKAHE
                     AQSQMKAEYEKQLAAVNARRDEALYQMKLLQNASLDAWQDMMRGTEEAWKNMSEAFNK
                     ARSHFENK"
     gene            2880..3815
                     /locus_tag="AC16O9_14080"
     CDS             2880..3815
                     /locus_tag="AC16O9_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517779.1"
                     /translation="MTTSLVEGASILAIMTPVFFPDFSLEHVAVSTGTLRLRRGGEGP
                     PLLLLHGNPQTHAMWHKVAPALARNHTVVCPDLRGYGGSFKPPVSADHAAYAKREMAK
                     DMAELMTHFGHDAFLVAAHDRGARVAHRLALDHPQRVQKLALMDIIPTLEHFERTDMA
                     MAMGYYHWFWFAQPHPFPENVISAAPDAWWRAHTSREPKPPDFFDREALADYMSAVHD
                     PEMIRGMCEDYRAAATIDLVHDRASRAAGEKIRCPLLVLWGKKAKIEAWYDALAIWRT
                     YSAAGVSGGSVNSGHYLAEEAPSEVLARLLPFLSA"
     gene            complement(3807..4159)
                     /locus_tag="AC16O9_14085"
                     /pseudo
     CDS             complement(3807..4159)
                     /locus_tag="AC16O9_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
     gene            complement(4241..5305)
                     /locus_tag="AC16O9_14090"
     CDS             complement(4241..5305)
                     /locus_tag="AC16O9_14090"
                     /inference="COORDINATES: protein motif:HMM:NF016071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HIR6517780.1"
                     /translation="MKKAGVIRSLIAAAVIAAFAGSAGADTIDEVETNSPVTAAQALV
                     FDASGAAVVNAFIADGDGDVFAFQAKEGDIVTVDINAAVMGSPVDTFVTVLQTQALGH
                     IVFRDNDDGPDGSGDSYIANIVIPADGVYYVGVAGYPNRVADGGVLRGGPGNATGAYT
                     LIVSGVSPVVTQTAPEPNTSTQTEASTQTEASTQTQTESGPVTSPFPQQTVDVLSIGI
                     DIKPGVRHIAKIDPRSPRDIPVALLGSRNFNVRDVDPGSLTFGSTGDERSLKRCNQRL
                     TRVNRGRRPDLICIFENQESGFQATDEEGVLRGKLKDGTPFEGRGMLKVIAEKRKSHR
                     HDWRHDHRRDRDDDRRKNRR"
     gene            complement(5492..6631)
                     /locus_tag="AC16O9_14095"
     CDS             complement(5492..6631)
                     /locus_tag="AC16O9_14095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517781.1"
                     /translation="MDFAFSDEQEAIREAIAKICARFPDEYWMKKDKEGGFPSELHQA
                     LAKDGWLGIAMPEAYGGSGLGITEAAVMMQAIAESGAGFSGASAVHMNIFGLNPVVVF
                     GNDAQKKRMLPGLIAGKERACFAVTEPNTGLNTRKIKTLAKREGRRYVISGQKVWIST
                     AQVAEKILVLTQAQAGLTLFYTDFDRKHIEVREIEKMGRKAVDSNQVFFDGLPVPEED
                     RIGEEGKGFEYILHGMNPERILIAAECVGLGRCAVKRAARYANERIVFDRPIGKNQAI
                     QHPLAANWMALEAANLMVFKAAWLYDRKKACGAEANASKYLAAEACFDACQQAVMTHG
                     GYGYAKEYHVERYLRESLVGRIAPVSPQMILCFIAERVLGLPKSY"
     gene            complement(6632..7009)
                     /locus_tag="AC16O9_14100"
     CDS             complement(6632..7009)
                     /locus_tag="AC16O9_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6517782.1"
                     /translation="MARSRYHVRPTDVTPYSPANHTGTRNFRLIGRETVGATQMEMLI
                     GEVERGKGALPHAHPGIEQVCYLLEGAAHVEVGGEKFDLAPGEACFFPADLPHVFTAT
                     SERAKVMVIYAPPYEENPARRST"
     gene            complement(7009..7728)
                     /locus_tag="AC16O9_14105"
     CDS             complement(7009..7728)
                     /locus_tag="AC16O9_14105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517783.1"
                     /translation="MIHALFDNDTALVTGSGNGIGRAIAEALRAEGVRVLGADLKGSD
                     IDVDLSKPGEARRLAAEAIAKLGKVTIFVHSASPRRHEEDTPLEVSEATWRAMQEVNL
                     NAGFLLGQALGRHMREQGIRGRMLYITSLHAYAPRNLPHYSAAKAGQTMLVREFARAL
                     GKDGIRVNAIAPGAIPGGGFDAAKNPGLLATIPMGRFGTPADVAQMALAVLSERFGAY
                     VTGATIAVDGGIAQHNWIERR"
     gene            complement(7725..10811)
                     /locus_tag="AC16O9_14110"
     CDS             complement(7725..10811)
                     /locus_tag="AC16O9_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6517784.1"
                     /translation="MQLPELCIKRPVFATVMSLMIVLLGVISFQRLTVREYPKIDTPV
                     VSVRTVYKGASAQVIESQVTQPLEDSLAGIEGVRTLKSVSRVEVSQITVEFVSERDVD
                     AAANDVRDRVARVRARLPEAADESVVSKIEADAQAIMWLAFFSERHGSLELSDYADRY
                     VADRLKTLTGVASVIIGGARQYAMRLWLDRERLAAYALTVQDVENALRRQNVEIPAGR
                     IESTQREFTVLAESDLRTAEEFNKLIIAERQGYPVRLRDVGVARLGALDERNIVRVNG
                     NPAVGLGVVKQSTANTLEVARAVKAEIERIRESLPEGMTLRVAFDSSIFIERSIDAVY
                     KVLGEAVLLVVLVIFLFLRSVRSTLIPFVTIPVSLVGAFFFLYLLGFSVNVLTLLGIV
                     LAVGLVVDDAIVVLENCHRHVELGKPTRLAAIDGSREIAFAVVAMSLTLVAVFAPLAF
                     QQGRTGRLFVEFALTVAGAVAVSGFVALTLTPMMCSRLLRAHERHGRVYLAMEGFFTG
                     MTSGYRSVLGGALRRRWIVGLAFLAILGGTAFTYLALKKELSPLEDRGFFLSFILAPE
                     GASLAYTDANVRAVERIYAGVPEIVNTFAVVAPGLERPNPVNLGITFSQFKPWEQRTR
                     RTQDITAELRPKLFAGVPGVFVFPVDPPSLGQSFRNPPVAFVVQADSYETLDKAGSAL
                     LAEARQSKMLVNADTDLKLNKPQLAVDIDRERAAALGVDIETIGRTLETLLGGRQVTR
                     FKREGKQYDVMVQLEARDRNTPADLTAIYVRARDGRLVQLSNLVTVRETVAAKELNHF
                     DRQRALILQANVAPGHSLGEALEFLEQAAAKVLPKSARTGLDGQSREFYEAGTTLLFT
                     FGLALAFIYLVLAAQFESFVSPLIIMLTVPLAALGALGTLWATGNSINVYSQIGLVML
                     IGLITKNGILIVEFANQLRARGMRRIEAVIESSTLRLRPILMTSLATVLGALPLALAA
                     GAGSEARQAIGWVVVGGLSVGTLFTLFVIPTAYTVLVRDAQPAPAAAPTAIKENQA"
BASE COUNT         1714 a         3769 c         3589 g         1781 t
ORIGIN      
        1 gggcacgccg aacaccaagg taaagaacac ccaggtgcac ggcgccgagg tggtgctgga
       61 gggcgacacg ctgtcggact ccggcgcgca cgcgcgcgag ctggcggcgc gcgaaggcta
      121 taccttcgtc cacccctacg atgacgccgc ggtcgtcgcc ggccagggca ccgtcggcct
      181 ggagatgctc gaggacgtcc caggcctgga gatgctggtc gtgccggtgg gcggcggcgg
      241 cctcgcctcg ggcatcgcca ccgcggccaa ggcgctgcgt cccgagacgc acgtatacgg
      301 cgtcgagtcg aaagcctgga gcgccatgtt ccagcgcatg cacggcctgc cggtggacgt
      361 gggcggcgac accatcgccg aaggcatcgc ggtgcgcgac gtgagcgcgc tgacgctcga
      421 gatctgcaag cgcttcctcg acgaagtgct gctggtcgat gaggagatca tcgagcgcgc
      481 catcgtcgcg ctgatcgaga tcgagaagac ggtggccgag ggcgcgggcg cggccggcct
      541 cgccgccgtg ctcgctcatc cgcagcgctt cgccggccgg cgcgtcggca tcccgatttg
      601 tggcggcaac atcgactcgc gcgtgctgtc ggcggtcctc atgcgcgggc tggtgcgcga
      661 cggccgcctg gtgcgcatgc gcgtcatgat gcccgatctg tccggcagcc tggccaaggt
      721 ggcggcgctg atcgccgcag ccggcggcaa catcgtcgag gtacagcacc agcgcgtctt
      781 cggcaccgcc tcggtgagga ctccggaagt cgagttcgtc atagagacgc gcgaccgcga
      841 gcacacacgc gccatcgtcg cacacctgag cacgcacggc gccacggcgc ggctggacac
      901 ctagcccacg ggctccgaga tctcgaccag gttgaggtcg ggatcgcgca cgtacaccga
      961 gcggatcttc gacacggcgc cggtcttcat caccggccct tcgaggatct tcacgttcgc
     1021 ggcattcagc ctcgcgatca cctgctcgag gggcaccgag gcgataaagc acaagtcgag
     1081 cgtcccgggc gccgcgacgt gcgcgcgcgg cacgaactcc ttgccccact cgtgcaggtt
     1141 gattttctgg ttgccgaatt tcagcgcctg gcgggtttcc gtgggcgtcc tgaacgattc
     1201 cagcgccatc ccgagcacat cgcgatagaa gcgcacgcag ccctccaggt cgcgggtggt
     1261 cagaacgata tggtcgacgt ggtcaatcat aaatggggac agacctcatt tttcttatct
     1321 gcgaacaaag tgcaaatgag aaaaatgagg tctgtcccta ttttcgtttc agcatgcgca
     1381 gcgggttgta ctcgatgacc gtttcgccgc gctgattcac caccttgttg gcaaagcgca
     1441 ccaggccgcg gtcgcctttc gaggtcagcc gcgcctcggt cacctcgacc tcgacgtgaa
     1501 tggtgtcgcc gactaccgtc ggccccttca cgtcgagcgt ggcgttgagg aacgcgagcc
     1561 ccgtgtcctg catcgacggc agcagcagcc cctcggcgaa ggaatagacc agcgcgcccg
     1621 gtaccggccg ccccttgatg gcgcgctgtg agtcgtcctc gaccgcgaac agctcctcgg
     1681 tgagccaggt caggttgacg aagcccgcca ggtccgcttc cgcgatggtg cggcggtgcg
     1741 tgcgaaagcg catgccgacc ttcaggtcct cgtagcagta gccgcggccg atcgggtgaa
     1801 cgttgtcgct catgtcagaa gatcgtgtag gcgccgtcga tgacgaacga atcgcccgta
     1861 tggtagccgc tcgcgtcgct catcaggtac acggcgatac cggcaaagtc ctccttcttt
     1921 ccccagcgcc gcagcgggat gcgcggcagg acggcggcgg agaacttcgc atcgccgatc
     1981 gctgccgccg tcatgtccga ctcgatccag cccggcagga tggagttggc ggtgacctgg
     2041 aagcgcgcca tctccaccgc gagcgcgcgg atcatggtga gcatcgcgcc cttggtcgcg
     2101 gcgtagctcg acgcgcgcgc cgcgccgtgg atcgcggcgc tgctcgccat gccgacgagc
     2161 cgtccgcccg cgtcgcccgc caccgcccgg ccggtcatgt ggcgcgccgc ggcacgcagc
     2221 gtaaagaacg cgccgtccag gtttacctgc atcacgcggc gccattcctt cgtcggcagc
     2281 tcggcgagcg gcgcgcgcac ctggctcacc ccggcgttgg cgaagcagcc gtcgacgcgg
     2341 cccattgcct tcagcgcctc ggcgaagctt cgttcgacct gcgcctcgtc gctcacgtcg
     2401 cagcgctgcg ccagcacgcg ccggccgtgc tttttgagca gcttctcggc ggcggcgttc
     2461 ttgtccgggt tcgaccccca gatcaccacg tcagcccctg cggcggcgag gccttcggcc
     2521 atgcccaggc cgatgccgcc gttacccccc gtgacgagcg cgaccttgcc tttcaggtcg
     2581 aatgcgctca tttgttctcg aagtgcgacc tcgccttgtt gaacgcctcg ctcatgttct
     2641 tccacgcctc ttcggtgccg cgcatcatgt cctgccaggc gtcgagcgac gcgttctgca
     2701 gcagcttcat ctggtagagc gcctcgtcgc ggcgagcgtt gacggcggcg agctgcttct
     2761 cgtactcagc cttcatctgc gactgcgcct cgtgcgcctt cgcctcccac tgcttggcct
     2821 cggcattcca ctggtcgagc tgggccttca gcttccgcac gtactcgtcg cggttcatca
     2881 tgacgacctc cttggtggaa ggagcttcga ttttagccat aatgactccc gtgttcttcc
     2941 ctgatttctc gctggagcac gttgccgtgt ccaccggaac gctgcgcctg cgccgcggcg
     3001 gcgaagggcc gccgctcctg ctcctgcacg gcaacccgca gacgcacgcc atgtggcaca
     3061 aggtggcgcc ggcgctggcg cggaaccaca ccgtggtctg tcccgacttg cgcggctacg
     3121 gcggctcgtt caagccgccc gtcagcgccg accacgcggc gtacgccaag cgcgaaatgg
     3181 ccaaggacat ggcggagctg atgacccact tcggccacga cgcgttcctc gtcgccgcgc
     3241 acgatcgcgg cgcgcgggtc gcgcaccgcc tcgcgctcga ccatccgcag cgcgtgcaga
     3301 aactcgcgct catggacatc atccccacgc tcgagcattt cgagcgcact gacatggcga
     3361 tggccatggg ctactaccac tggttctggt tcgcgcagcc gcacccgttt cccgaaaatg
     3421 tgatcagcgc cgcacccgat gcctggtggc gcgcgcacac ctcgcgcgag cccaagcccc
     3481 ccgatttctt tgaccgcgag gcgctcgccg actacatgag cgcagtccac gacccggaaa
     3541 tgatccgtgg catgtgcgag gactaccgcg ccgcggccac gatcgacctc gtgcatgacc
     3601 gcgccagccg cgcggcgggc gagaagatcc gctgcccact gctcgtgctg tgggggaaga
     3661 aggcgaagat cgaggcctgg tatgacgcgc tcgccatctg gcgcacctac agcgccgccg
     3721 gggtcagcgg ggggtcggtg aattcgggcc actacctcgc ggaggaagcg cccagcgagg
     3781 tgctcgcacg cctgctgccg tttctctcag cctgacgcca gtaccagcgc tagcgccacg
     3841 gcgcccggca gcgcctgaac ccacaggatg cgccggctca cgctccacgc gccgtagacg
     3901 ccggcgacga tcacgcaggc gagaaagaac accttgaacg gctgcgagcc ggcgatcagg
     3961 cccaggcgag ccctgcagcc agaaagccgt tgtagagccc ctggttggca gcgagcacct
     4021 tcgaagcaag cgcgaactca ggggtctggc gaaacacctt ccggccgaat ggcttgtccc
     4081 agaggaacat ctccaggacg aggaacgcca gatgcagcag cgccaccatg gcggtagcca
     4141 agtcggcgag cactcccatg gcgcgggagt gtacgcacta aaggaaacgg ccgccgcagc
     4201 ggggggctgg ggcggccgtc gggggcagag cgaggaagga ctagcggcgg ttcttcctgc
     4261 gatcgtcgtc gcggtcgcgc cggtgatcgt ggcgccagtc gtgacggtgg ctcttgcgct
     4321 tctcggcgat gaccttgagc atgccgcggc cttcgaaggg ggtgccgtcc ttcagcttgc
     4381 cccggagcac gccttcctcg tcggtcgcct ggaaaccgga ttcctggttc tcgaagatgc
     4441 agatcaggtc cggccgccgc ccgcggttga cgcgcgtgag gcgctggttg cagcgcttca
     4501 ggctgcgttc gtctccggtg ctaccgaaag tcagcgagcc cgggtccacg tcgcgcacgt
     4561 tgaagttccg cgagccaagc agcgccaccg gaatgtcgcg cggcgacctc ggatcgatct
     4621 tcgcgatgtg ccgcacgccc ggcttgatgt cgatgccgat gctcagcacg tcgaccgtct
     4681 gctgcgggaa aggcgaggtc accgggccgg actcggtctg ggtctgcgtc gaagcctcgg
     4741 tctgcgtcga agcctcggtc tgcgtcgaag tgttgggctc gggcgcagtc tgggtcacca
     4801 cgggcgacac gccgctcacg atgagcgtat aggcgccagt cgcgttcccc ggcccgccgc
     4861 gcagcacgcc gccgtccgcg acgcggttcg ggtagccggc cacgccaacg tagtacacgc
     4921 catccgcagg gatcacgatg ttcgcgatgt aggagtctcc gctcccgtcc ggaccgtcgt
     4981 cgttgtcacg gaacacgata tggcccaggg cttgggtctg cagcaccgtc acgaaggtgt
     5041 cgacagggct ccccatgacg gccgcgttga tgtccacggt gacgatgtcg ccctccttcg
     5101 cctggaaggc gaacacgtcg ccgtcgccgt cggctatgaa ggcgttgacc acggccgcgc
     5161 cgctcgcatc gaacacgagg gcctgcgccg cggtgaccgg gctgttcgtc tcgacttcgt
     5221 cgatcgtatc cgcgcccgcg ctccctgcga aagccgcgat gacggccgca gcgatgaggc
     5281 tgcgaatgac tcctgctttc ttcatggtcc tctcccgcta tgtgtcaaat gtcacatgcc
     5341 ttttcggggc atgcgcaacg actgtactgg gggggcatgc ccccccacag ggaggaatcc
     5401 gcaggggtcg tcagaaatct tcagggcgca gacaagcggc gccagggagc ggtccggcgg
     5461 ccgcccgccg ccggtcaaaa ttcacgcgcg gctagtacga cttcggcagg ccgagcacgc
     5521 gctcggcgat gaagcacagg atcatctgcg gcgacacagg agcgatgcgc ccgaccagcg
     5581 actcccgcag atatctttcc acgtgatatt ccttggcgta gccgtagccg ccgtgcgtca
     5641 tcacggcctg ctggcaggcg tcgaagcagg cttcggcggc aaggtacttc gaggcgttcg
     5701 cttcggcgcc gcacgctttc ttccggtcgt agagccaggc ggccttgaac accatcaggt
     5761 tggcggcctc cagcgccatc cagttcgccg ccagcgggtg ctggatcgcc tggttcttgc
     5821 cgatcgggcg atcgaacacg atgcgctcgt tcgcgtagcg cgcggcgcgc ttcacggcgc
     5881 agcgccccag gcccacgcac tcggcagcaa tgaggatgcg ctccgggttc atgccgtgca
     5941 ggatgtactc gaagcccttg ccctcctcgc cgatgcggtc ttcctccggc accggcaggc
     6001 cgtcgaagaa cacctggttg gagtccaccg ccttgcggcc catcttctcg atctcgcgca
     6061 cctcgatgtg cttgcggtcg aagtcggtat agaagagcgt gaggccggct tgcgcctgcg
     6121 tcagcaccag gatcttctct gccacctggg cggtcgagat ccacaccttc tgaccggaga
     6181 tgacgtagcg cctgccctcg cgcttcgcca gggtcttgat cttgcgcgtg ttgagcccgg
     6241 tgttcggctc ggtgacggcg aagcaggcgc gctccttgcc cgcgatcagc cccggcagca
     6301 tgcgcttctt ctgcgcgtcg ttgccgaaca cgacgaccgg attgagcccg aaaatgttca
     6361 tgtgcaccgc cgaggcgccc gagaatccgg caccggattc ggcgatcgcc tgcatcatca
     6421 ccgcggcctc ggtgatgccg aggcccgagc cgccgtaggc ttccggcatc gcgatgccga
     6481 gccacccgtc tttggcgagc gcctggtgca gctcggaggg gaagcccccc tccttgtcct
     6541 tcttcatcca gtactcgtcg ggaaagcgcg cgcagatctt cgcaatcgcc tcgcggatcg
     6601 cttcctgctc gtcgctgaag gcgaaatcca ctcaggtgct ccgccgcgcg gggttttcct
     6661 cgtacggcgg cgcgtagatc accattacct tcgcgcgttc gctcgtggcc gtgaagacgt
     6721 gcggcaggtc ggcgggaaag aagcacgcct cgcccggcgc caggtcgaac ttctcgccgc
     6781 ccacctcgac gtgcgcggcg ccttcgagca ggtagcacac ctgctcgatg cccggatgcg
     6841 cgtgcggcag cgcacccttg ccgcgctcca cctcgccgat cagcatctcc atttgcgtcg
     6901 cgcccaccgt ctcgcggccg atgaggcgga agttccgggt gcccgtgtgg ttcgcgggcg
     6961 aatacggcgt cacgtcggtc gggcggacgt ggtagcggga gcgcgccatc agcgccgctc
     7021 gatccagttg tgctgcgcga tgccgccgtc caccgcgatg gtcgcgcctg tgacgtaggc
     7081 gccgaagcgc tcggagagca ccgcgagcgc catctgcgca acgtccgccg gcgtgccgaa
     7141 acgtcccatc ggaatggtgg cgagcaggcc cgggttcttc gccgcatcga agccgccgcc
     7201 cgggatcgcg ccgggggcga tggcgttcac gcggatgccg tccttgccca gggccctcgc
     7261 gaattcgcgc accagcatcg tctgcccggc cttggctgcg ctgtagtgcg gcaggttcct
     7321 cggcgcatag gcgtgcagcg aggtgatgta gagcatgcgg ccgcggattc cctgctcgcg
     7381 catgtggcgc ccgagcgcct ggccgagcag gaagcccgcg ttcaggttca cttcctgcat
     7441 cgcgcgccag gttgcctcgc tcacctcgag cggcgtgtcc tcctcgtgcc ggcgcgggct
     7501 cgcgctgtgc acgaagatcg tgaccttgcc gagcttcgcg atcgcttccg ccgccaggcg
     7561 cctcgcttcg cccggctttg acaggtcgac gtcgatgtcc gagcccttca ggtcggcgcc
     7621 gagcacgcgc acgccctcgg cgcgcagcgc ttcggcgatg gcgcggccga tgccgttgcc
     7681 cgagcccgtg acgagcgcgg tgtcgttgtc aaagagcgcg tgaatcatgc ctggttctcc
     7741 tttatcgcgg tgggcgccgc cgccggcgcc ggctgcgcgt cgcgcaccag caccgtgtag
     7801 gccgtcggaa tgacgaacag cgtaaagagc gtccccaccg acaacccgcc gaccaccacc
     7861 cagccgatcg cctggcgcgc ctcggaaccc gcgcccgccg ccagcgctag cggcagggcg
     7921 ccgagcacgg tggcgagcga ggtcatcagg atcggccgca ggcgcagtgt cgaggactcg
     7981 atcaccgcct cgatgcggcg catgccgcgc gcacgcagct ggttggcgaa ctcgacgatc
     8041 aggatgccgt tcttcgtgat caggccgatg agcatcacca ggccgatctg cgagtacacg
     8101 ttgatcgagt tgcccgtggc ccacagggta ccgagcgcgc caagcgcggc gagcggcacc
     8161 gtcagcatga tgatgagcgg cgagacgaag ctctcgaact gcgcggcgag cacgaggtag
     8221 atgaacgcca gcgcgagacc gaaggtaaac agcagcgtcg tgccggcttc gtagaactcc
     8281 cgcgactggc catccaaccc ggtgcgcgcg ctctttggca gcaccttggc cgccgcctgc
     8341 tcgaggaact ccagcgcctc gccgagcgag tggcccggcg cgacgttcgc ctgcaggatc
     8401 agggcgcgct ggcggtcgaa gtggttgagc tctttcgccg ccacggtctc gcgtaccgtg
     8461 acgagattcg acagctgcac gaggcgccca tcccgcgcgc gcacgtaaat cgcggtcagg
     8521 tccgccggcg tgttgcggtc gcgcgcctcg agctgcacca tgacgtcgta ctgcttgccc
     8581 tcgcgcttga agcgcgtcac ctggcgcccg ccgagcagcg tctcgagcgt gcggccgatg
     8641 gtctcgatgt caacgccgag cgccgcggcg cgctcgcggt cgatgtcgac cgcgagctgc
     8701 ggcttgttca gcttcaggtc ggtatcggcg ttgaccagca tcttgctctg gcgcgcttcg
     8761 gcgagcagcg cgctgcccgc cttgtcgagc gtctcgtagc tgtcggcctg caccacgaac
     8821 gccaccggcg ggttgcggaa gctctggccg agcgagggcg ggtcgaccgg aaacacgaac
     8881 acgccaggca cgcccgcgaa cagcttcggc cgcagctcgg cggtgatgtc ctgcgtgcgg
     8941 cgcgtgcgct gctcccaggg cttgaactgg ctgaaggtga tgcccaggtt gaccgggttg
     9001 gggcgctcga gccccggcgc cactaccgcg aaggtgttga cgatctccgg cacgccggcg
     9061 tagatgcgct cgaccgcgcg cacgttggcg tcggtatagg cgagcgaggc gccctcgggc
     9121 gccaggatga acgacaggaa gaacccccgg tcctcgagcg gcgaaagctc tttcttgagc
     9181 gcgaggtagg tgaacgccgt gccgcccagg atggcgagga acgcgaggcc cacgatccag
     9241 cgccggcgca gggcgccgcc gagcacgctg cggtagcccg acgtcatacc ggtgaagaat
     9301 ccctccatcg ccagatagac ccggccatgc cgctcgtggg ctctgagcag ccgcgagcac
     9361 atcatcggcg tgagcgtgag cgcgacgaag cccgacaccg cgactgcgcc ggccaccgtc
     9421 agcgcgaact cgacgaacag gcgcccggta cgaccctgct ggaaggcgag cggcgcgaac
     9481 accgcgacca gcgtgagcga catggcgacc acggcgaagg caatctccct ggagccgtcg
     9541 atcgcggcga ggcgcgtcgg cttgcccagc tcgacatggc ggtggcagtt ctcgagcacc
     9601 acgatggcgt catccaccac cagccccacc gccagcacga tgccgagcag cgtcagcacg
     9661 ttgaccgaga acccgagcag gtagagaaag aagaacgcgc cgacgagcga caccgggatg
     9721 gtgacgaacg ggatcagcgt cgagcgcacc gagcgcagga agaggaagat gaccagcacc
     9781 accagcagca ccgcctcgcc cagcaccttg tagaccgcgt cgatcgagcg ctcgatgaag
     9841 atggacgagt cgaaggcgac gcgcagcgtc atgccctcgg gcaggctctc gcgtatgcgc
     9901 tcgatctcgg ccttcaccgc gcgcgccacc tcgagcgtat tcgcggtcga ctgcttgacc
     9961 acgccgagcc cgaccgcggg attgccgttg acgcgcacga tgttgcgctc gtcgagcgcg
    10021 cccaggcgcg ccacgcccac gtcgcgcagc cgcaccgggt agccctggcg ctccgcaatg
    10081 atcagcttgt tgaattcctc ggcggtgcgc aggtcggact cggcgagcac cgtgaactcg
    10141 cgctgcgtgg attcgatgcg cccggccggg atctcgacgt tctggcgccg cagcgcgttc
    10201 tccacgtcct gcacggtgag cgcgtaggcc gcgagccgct cgcggtcgag ccacagccgc
    10261 atcgcgtact ggcgcgcgcc gccgatgatc accgacgcca ccccggtgag cgtcttcagc
    10321 cggtcggcga catagcgatc ggcgtagtcc gagagctcga gcgagccgtg gcgctccgag
    10381 aagaaggcga gccacatgat cgcctgcgcg tcggcctcga tcttcgacac caccgactcg
    10441 tccgccgcct cgggcagccg cgcgcgcacc cgcgccaccc ggtcgcgcac gtcgttggcc
    10501 gccgcgtcga cgtcgcgctc ggagacgaac tcgaccgtga tctggctcac ctccacgcgc
    10561 gacaccgatt tcaacgtgcg cacgccctcg atgccggcga gcgaatcttc gagcggctgc
    10621 gtcacctgcg actcgatcac ctgcgcgctc gcgcccttgt agacggtgcg taccgagacc
    10681 accggcgtgt cgatcttcgg gtactcgcgc accgtgaggc gctggaacga gatgacgcca
    10741 agcagcacga tcatcaggct catcaccgtc gcgaagacgg ggcgcttgat gcagagctcg
    10801 gggagctgca tggaccttgc cttggccttt gaccttagac gatctcgcct ttt
//
LOCUS       DBNHKE010000206         5232 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1148529, whole genome shotgun sequence.
ACCESSION   DBNHKE010000206 DBNHKE010000000
VERSION     DBNHKE010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1148529"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..439)
                     /locus_tag="AC16O9_14115"
     CDS             complement(104..439)
                     /locus_tag="AC16O9_14115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6517785.1"
                     /translation="MPADANHNGDIFGGWIMAQVDVAGGTVAGRVARGRVATVSVNSF
                     VFKQPVQIGDILSLYADVVRIGNTSITVNVEVFAERGRAESRVVKVTEATLTYVAIDG
                     KGAPRPVPK"
     gene            complement(527..2479)
                     /locus_tag="AC16O9_14120"
     CDS             complement(527..2479)
                     /locus_tag="AC16O9_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR6517786.1"
                     /translation="MDRGQTGTTLKHWRWEQDREGLAWLTFDKQGESTNTFSREALEE
                     LGAALGVLAAAKPKGLVIRSAKENFIAGADINQFTRFKTPEEALEFTRLGWDTLQKLR
                     DLPFPSTAMVNGFCMGGGLELALACTYRVALDDPKTRFSFPEVMLGIWPFWHGLQWLP
                     KLVGPSAGLDMILTGRAIDARRAKRIGLADESVPLRVLENTARLVTLQAKPRKALGLA
                     DRVMLGVGRSFVAAQARKQVARRVRREHYPAPYAILEAWVKYDGDPFVAPDDPVCSIK
                     ALSTHPTTRNLIRIFFLQERMKGLAKGVDFKAQHVHVIGAGVMGGDIAAVCAMRGIRV
                     TLQDTSPERLAPAMKRAAELFEKRLKDKRRVRDALDRLIPDVSGAGAARADVVIEAIF
                     ENLEAKRELFAKVESVAKPGAVIATNTSSLKLADVAAQFKDPSRLVGIHFFNPVPQLQ
                     LVEVVTGARSSEGMVRKAMAFVRQIDKLPLPVKDSPGFLVNRVLGPYMQNAFRKLDEG
                     VKPETLDAAMEAFGMPMGPMELADTVGLDICVAAGRELAKKSAGGAPEAPKALLDKVA
                     LGQLGKKTGQGIYRYEKGKPAKGQPDAYGSELVEALIEPYLNEAKAVRDEGIVADAEL
                     IDAGLIFGTGFAPFRGGPLHYLESQK"
     gene            complement(2482..3780)
                     /locus_tag="AC16O9_14125"
     CDS             complement(2482..3780)
                     /locus_tag="AC16O9_14125"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6517787.1"
                     /translation="MIRQPIYVIDGARTPFLKSKNRPGPFAAGDLAVQAGRALLVRQK
                     FAPEQFDEVILGCAAPSVDEVNIGRVAALRMGCGEKVPGWTVMRNCASGMQALDSGIN
                     NILAGRSQLVLAGGVDALSRAPLLYNDRMVNWFSDLAAAKTTGQRAALFARMPFKALL
                     APVIGIMKGLTDPMVGLLMGQTAENLAHRFGITREQMDAFAVRSHQRVARAQDEGPLK
                     PGGGEVEALYDARGNSYGLDDGVRRDASMQGLAKLKPFFDRKYGNVTPGNSSQITDGA
                     AWLILASEEAVKRHDLTPIGRIVDSQWAGLDPAQMGLGPVHAATPILQRHRLGLDDLD
                     FWEINEAFAAQVLGCVAAWKDENYCKEQLGLDRAMGELDQDRLNVDGGAVALGHPVGA
                     SGARIVLHMLRLLKRKNAKRGMASICIGGGLGGAMLLEAA"
     gene            complement(3782..>5232)
                     /locus_tag="AC16O9_14130"
     CDS             complement(3782..>5232)
                     /locus_tag="AC16O9_14130"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF007000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6517788.1"
                     /translation="GKDVFVPLEWIIGGREYAGKGWMMLMNSLAAGRSISLPASSAGG
                     AKALARVTGAYARVRSQFKTPIGKLEGVEEALGRIAANAYMMEATRVMTAGAVDQGEK
                     PSVISAIAKYHMTERARTCVNDAMDIHGGKGICLGPNNWVGRGYQVLPVGITVEGANI
                     LTRTLIIFGQGAIRAHPYVLREMRAAKELQGTEASIEFDDAFTAHIGHTLSNGVRAFL
                     HGLTRSMFAAAPLNAARETRHNYRHVTRLSAAFAFLADISMLAMGGALKRKEKISGRL
                     GDVLSMMYLVSATLKRYEDQGRLQADLPLVRWSVREALYSAQEAIDQVLSNFPIKALA
                     TFLRWTIFPLGTPFRPPLDSRNHECARIALEPGAARDRLTAGIYLPKGEADATGVLEA
                     AFLAAVACEPIEKKFRAAVKAGTLTPRSGTDTAQLARDRGVITPEEFAQWQRKEALRK
                     RVIMVDDFPQDYGRAEIAQKLRDEQPTHAKAA"
BASE COUNT          751 a         1839 c         1792 g          850 t
ORIGIN      
        1 gccgcctgga acagcaccgc gcggcggttc ggcgtccgcg gccggtagag tgtgccggcg
       61 aggcgatagc cgtcgcgggc ggcgaattcg accgcggtgc cgctcacttc ggcacggggc
      121 gcggcgcgcc tttgccgtcg atcgcgacat aggtcagggt cgcttcggtg actttcacga
      181 ccctcgactc ggcgcggccg cgctcggcga atacctcgac gttcacggtg atcgaggtgt
      241 ttccgatgcg tacgacgtcg gcatacaggg acaggatgtc gccgatctgc accggctgct
      301 tgaagacgaa ggagttcacc gacaccgtgg cgacgcgccc gcgcgccacg cgccccgcga
      361 ccgtgccgcc ggcgacgtcc acctgcgcca tgatccagcc gccgaagatg tcgccgttgt
      421 ggttggcgtc ggccggcatc ggcacgacgc gcagcgccgg gaccttgccc gcaggcaact
      481 ccaggtccgg cggctggtgc gacgcgcgac ccacggcgtc ccgcgcctac ttctgcgact
      541 cgaggtagtg cagcgggccg ccgcggaacg gcgcgaagcc ggtgccgaag atcagccccg
      601 cgtcgatcag ctcggcgtcg gcgacaatgc cctcgtcacg caccgctttc gcctcgttga
      661 ggtacggctc gatgagcgcc tcgaccagct ccgacccgta cgcatccggc tgccccttcg
      721 ccggcttgcc cttctcgtag cgatagatgc cctgaccggt cttcttgccg agctgcccga
      781 gcgccacctt gtcgaggagc gccttcggcg cctcgggcgc gccgcccgcg ctcttcttcg
      841 ccagctccct gccggcggcc acgcagatgt cgaggcctac cgtgtcggcg agctccatcg
      901 ggcccatcgg catgccgaag gcctccatcg ccgcatcgag cgtctccggc ttcacgccct
      961 cgtcgagctt gcggaaggcg ttctgcatgt agggcccgag cacgcggttg acgagaaagc
     1021 cgggagaatc cttcaccggc agcggcagct tgtcgatctg gcgcacgaac gccatcgcct
     1081 tgcgcaccat gccttcgctc gaacgcgcgc ccgtgaccac ctcgacgagc tgcagctgcg
     1141 gcacggggtt gaagaagtgg atgccgacga ggcgcgaggg atccttgaac tgcgccgcga
     1201 catcggcgag cttgaggctc gaggtgttgg tcgcgatgac cgcgccgggc ttggccaccg
     1261 actccacttt cgcgaacagc tcgcgcttgg cctcgaggtt ctcgaagatc gcctcgatga
     1321 cgacgtcggc gcgcgccgcg ccggcgccgg agacgtcggg aatcagccgg tcgagggcgt
     1381 cgcgcacgcg ccgcttgtcc ttcaggcgtt tctcgaacag ctcggcggcg cgcttcatgg
     1441 ccggcgccag ccgctcgggc gaggtgtcct gcagcgtgac gcggatgccg cgcatggcgc
     1501 acaccgcggc gatgtcgccg cccatgacgc ccgcgccgat gacgtgcacg tgctgcgcct
     1561 tgaagtcgac gcccttggcg aggcccttca tcctctcctg caagaagaag atgcggatca
     1621 ggttgcgcgt ggtcggatgg gtggagagcg ccttgatcga gcacacggga tcgtcgggcg
     1681 cgacgaacgg gtcgccgtcg tacttcaccc aggcctccag aatggcgtaa ggcgccgggt
     1741 agtgctcgcg gcgcacgcgc ctggcgacct gcttgcgcgc ctgggcggcg acgaagctgc
     1801 ggcccacgcc gagcatcacg cggtccgcca gtccgagcgc cttgcgcggc ttagcctgca
     1861 gcgtgaccag gcgcgcggtg ttctccagca cgcgcaaggg gaccgactcg tccgcgaggc
     1921 cgattctctt cgcgcgccgc gcgtcgatgg cgcggccggt caggatcatg tcgagtccgg
     1981 ccgacgggcc gaccagtttc ggcagccact gcaaaccgtg ccagaacggc cagatgccga
     2041 gcatcacctc gggaaaggaa aagcgcgtct tcggatcgtc gagcgccacg cgataggtgc
     2101 aggcgagcgc gagctcgagg ccgccgccca tgcagaaccc gttcaccatc gcggtgctcg
     2161 gaaacggcag gtcgcgcagc ttctgcagcg tgtcccagcc caggcgcgtg aactccagcg
     2221 cctcctccgg cgtcttgaag cgcgtgaact ggttgatgtc ggcaccggcg atgaaatttt
     2281 ccttcgccga gcggatgacg aggcccttgg gcttggccgc ggcgagcacg ccgagcgccg
     2341 cgcccagctc ctccagcgcc tcgcgcgaaa acgtgttggt cgattcgccc tgcttgtcga
     2401 aggtgagcca ggcgaggccc tcgcggtcct gctcccagcg ccagtgcttg agtgtcgtgc
     2461 cggtctgccc gcggtccatg gtcaggcggc ctccagcagc atcgcgccgc ccagcccgcc
     2521 gccgatgcag atcgaggcca tgccacgctt cgcattcttg cgcttgagca gccgcagcat
     2581 gtgcagcacg atgcgcgcgc ccgaggcgcc caccgggtga ccgagcgcga cggcgccgcc
     2641 gtcgacgttg agcctgtcct gatcgagctc gcccatcgcg cgatcgagcc ccagctgctc
     2701 cttgcagtag ttctcgtctt tccaggcggc gacacacccc agcacctgcg cggcgaacgc
     2761 ctcgttgatc tcccagaagt cgagatcgtc gagccccagc cggtggcgct gcaggatcgg
     2821 ggtcgcggcg tggaccgggc cgagccccat ctgcgccgga tcgagccccg cccactgcga
     2881 gtcgacgatg cgcccgatcg gcgtcagatc gtggcgcttc accgcctcct cggaagcaag
     2941 gatgagccag gccgcgccgt cggtgatctg cgagctgttg ccgggggtga cgttgccgta
     3001 cttgcggtcg aagaacggct tcagcttcgc caggccctgc atcgaagcgt cgcgccgtac
     3061 gccgtcgtcc aggccgtagc tgttccccct cgcgtcgtag agcgcctcga cctcgccgcc
     3121 gccgggcttg agcgggccct cgtcctgggc gcgcgccacg cgctggtggc tgcgcaccgc
     3181 aaaggcatcc atctgctcgc gcgttatgcc gaagcggtgc gcgaggttct cggcggtctg
     3241 gcccatgagc aggccgacca tcgggtcggt gaggcctttc atgatgccga ttaccggcgc
     3301 gagcagcgcc ttgaacggca tgcgtgcaaa gagcgccgct cgctgcccgg tcgtcttcgc
     3361 cgccgccagg tcggagaacc agttcaccat gcggtcgttg tagagaagcg gcgcgcgcga
     3421 cagcgcatcc accccgcccg cgagcaccag ctgcgagcgc ccggcgagga tgttgttgat
     3481 gcccgagtcg agcgcctgca tgccgcttgc gcaattgcgc atcaccgtcc agcccggcac
     3541 tttctccccg cagcccatgc gcagcgcggc gacgcggccg atgttcacct cgtcgaccga
     3601 gggtgcggcg cagcccagga tcacctcgtc gaactgctcg ggcgcgaact tctggcgcac
     3661 cagcagcgcg cggccggcct gtactgccag gtcgcccgcg gcgaacgggc cgggccggtt
     3721 cttggatttc aggaacggcg tgcgcgcgcc gtcgattacg tagatgggtt ggcggatcat
     3781 gtcaggcggc cttggcgtgt gtcggctgct cgtcgcgaag cttctgcgcg atctcggcac
     3841 ggccgtagtc ctgcgggaag tcgtcgacca tgatgacgcg cttgcgcagt gcctccttgc
     3901 gctgccactg tgcgaactcc tccggcgtga tgacgccgcg gtcgcgcgcg agctgcgccg
     3961 tgtcggtgcc cgagcgcggc gtgagcgtac cggccttcac cgccgcgcgg aacttcttct
     4021 cgatcggctc gcaggccacg gcggcgagaa acgccgcttc cagcacgccg gtggcatcgg
     4081 cctcgccctt gggcaggtag atgcctgcag tgagccggtc gcgcgccgcg cccggctcga
     4141 gcgcgatccg cgcgcactcg tggttgcgcg agtcgagcgg cggccggaac ggcgtgccga
     4201 gcgggaagat ggtccagcgc aggaaggtgg caagcgcctt gatcgggaaa ttggagagca
     4261 cctggtcgat cgcttcctgc gccgagtaca gggcctcgcg caccgaccag cgcaccagcg
     4321 gcaggtcggc ttgcaggcgc ccctggtcct cgtagcgctt gagcgtcgcc gataccagat
     4381 acatcatgga gagcacgtcg cccaggcgac ccgagatctt ttccttacgc ttcagtgcgc
     4441 cgcccatcgc cagcatcgat atatcggcga ggaaggcgaa ggccgcggac agccgcgtca
     4501 cgtggcggta gttgtggcgc gtttcgcgcg ccgcgttgag cggcgccgcc gcaaacatgg
     4561 agcgcgtgag gccgtgcagg aaggcgcgca cgccgttgga cagcgtgtgc ccgatgtgcg
     4621 cggtgaaggc gtcgtcgaac tcgatcgagg cctcggttcc ctgcagctct ttcgcggcgc
     4681 gcatctcgcg cagcacgtag gggtgggcgc ggatcgcccc ctggccgaag atgatcagcg
     4741 tgcgggtgag gatgttcgcc ccttccacgg tgatgcccac cggcagcacc tggtagccgc
     4801 gcccgaccca gttgttcggc ccaaggcaga tgcccttgcc gccgtggatg tccatggcgt
     4861 cgttcacgca ggtgcgcgcg cgctcggtca tgtggtactt ggcgatggcc gagatcaccg
     4921 agggcttctc gccctggtcg accgcgcccg cggtcatgac gcgggtggcc tccatcatgt
     4981 aggcgttggc ggcgatgcgc ccgagcgctt cttcgacgcc ttcgagcttg ccgatcggcg
     5041 tcttgaactg gctgcgcacc cgggcatacg ccccggtcac gcgcgcgagc gccttggcgc
     5101 cgcccgccga ggacgccggc agcgagatcg agcgtccggc cgcgaggctg ttcatcagca
     5161 tcatccagcc cttgcctgcg tactcgcgcc cgccgatgat ccactcgagc ggcacgaaca
     5221 cgtccttgcc cg
//
LOCUS       DBNHKE010000207        16379 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_249839, whole genome shotgun sequence.
ACCESSION   DBNHKE010000207 DBNHKE010000000
VERSION     DBNHKE010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_249839"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1233
                     /gene="acdA"
                     /locus_tag="AC16O9_14135"
     CDS             49..1233
                     /gene="acdA"
                     /locus_tag="AC16O9_14135"
                     /EC_number="3.13.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-sulfinopropanoyl-CoA desulfinase"
                     /protein_id="HIR6517789.1"
                     /translation="MFELSDTQKALQQSARDLAKRKFAPRAAEIDRREEYPWHNVELL
                     TQAGFMGMTIPQEYGGQGLGYLEAVLVIEEMARVCGVTGRIVVEANMGAIGAIMKYGT
                     QAQKTLAAKLVLAGDKPAICITEPEAGSAATQMSTRADRKGGRYVLNGRKHWITGGGV
                     SKLHLIFARVFENGVEQGIGGFIAVRGEARGLVVGRREPAMGLRGIPECEILFEDLEI
                     PLEMMLVPPEGIRRGFAGLMNAYNGQRVGAATVALGIAAGAYELALEYAQTRKQFDRP
                     ICEFQGLQWILVDMHTQLAAARLLIHKAAAGSGDGFPDMTEAAQAKVFAADMAIKVTN
                     DALQVFGAAGYSRELPLERMVRDARMFTIGGGTAQILRTVIASQLLGRKLPQTRDGYR
                     NL"
     gene            complement(1230..3200)
                     /locus_tag="AC16O9_14140"
     CDS             complement(1230..3200)
                     /locus_tag="AC16O9_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR6517790.1"
                     /translation="MADAAEAAPIEARYRSLAPLWRVVLIVLVSGAIGLALNQLLNLG
                     FFVGKTLLDTAYLYWLCALLVGSVFLLVPAGKHARRNAVPWYDVLLFATSFAVFAYFA
                     LHAQRIVEEGWEYKAPAQAVWIAYVGWVMLLEATRRAGGNAVFVVVALISLYPVFAGH
                     MPGPIAGLPRDLASTASFHFASSESALGIPTRAFGELVIGFVMFGAVLQYTGAAHFFN
                     NLAFALFGSVRGGPAKVSIFASGLMGSVSGSVVSNVLTTGVVTIPAMKRIGFRPAYAG
                     GVEACASTGGVLMPPVMGATAFVMASFLGLPYVAIAVAALVPSLLFYFALFVQIDGYA
                     AKNGLKGLPRSELPSLGQTFLEGWHYLFVFALLVYMLLVLQRESLAPFYATALLLVIN
                     QFSRRYRLDWRRLRTLLEGVGGALSELAALLAGVGLIIGALSVTGLAGTLANDLVYLA
                     GNNIYVLLVMGAVTSFIFGMGMTITACYIFLAIVLAPPLVGAGFDPVAVHLFMLYWGM
                     VSFITPPVALAAFVAAGIAKAPPMQVGLQSVRLGAAMYFVPFFFVLNPALILRGTPWE
                     IVVVVGTAIAGIALIGSALEGYVVGIGAVARSALGWAARLLMLAGGLVMAMPGGGELG
                     LSHLQLSLAGLALAAAGAAITWLGARATVPSS"
     gene            complement(3215..4369)
                     /locus_tag="AC16O9_14145"
     CDS             complement(3215..4369)
                     /locus_tag="AC16O9_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6517791.1"
                     /translation="MTHALLRAALGATLAFSVAAHADVKLPPTISWSAYDVGSGGYNQ
                     AVAIGNALKQKYNVSLRVLPGKNDVSRNLPIREGQVQFSANGVGGAYLAMEGVFEFGA
                     PSWGPQPVRGLMLNTSDQVLTVVAAGDAGVRTIADLKGKRVAWVIGSPALNQNITAML
                     AFSGLTWDDVRKVEFGGFGAAMDGIINNQVDAAFTSSISGKAYQIAKSPRGLVYPIIS
                     HKDKAGWARMNAVAPFFFPFMGTEGAELSKDKPAESATYPYPILMTYASQDANLVYNM
                     TKAMVETYDMYKGAAPGNAGWAVDRQNFAWVIPFHDGAIRFWKEMGKWGAAEQAHNDK
                     LLERQTVLTAAWAEQKKGSYADDKAFVEAWQKRRAAALTKAGFDPVQTSW"
     gene            4441..5022
                     /locus_tag="AC16O9_14150"
     CDS             4441..5022
                     /locus_tag="AC16O9_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201021.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HIR6517792.1"
                     /translation="MSQPISRYPVPELKNLPEDVRARILAVQEKSGFVPNVFLVLAHR
                     PEEFRAFFAYHDALMEKEGGLTKAQREMIVVATSGANHCQYCVVAHGAILRIRAKNPL
                     VADQVAINYRKADIPPREKAMLDFAMKVALESHALGDSDLEALRRHGFTDEDAWDIAA
                     IAAFFALSNRMADFTAMRPNDEFYPMGRVPKEK"
     gene            complement(5027..6892)
                     /gene="meaB"
                     /locus_tag="AC16O9_14155"
     CDS             complement(5027..6892)
                     /gene="meaB"
                     /locus_tag="AC16O9_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR6517793.1"
                     /translation="MSGPLSGIVVLEVGHMLAGPYCGMLLADLGAEVIKIEPPEGDIG
                     RRVSPHQVGGHNAYFASLNRSKKSVALDLARDDGRAALRGLAARAHALVTNLRPAAIR
                     KLGLTYEALKDANPKLVCVALTGYGLEGSHADRPAYDYVIQALTGVMAITGEPGGAPA
                     KTGYSAVDNSAGMMGALGLLSKIVEGRGGQIDVSMHDVMLSQLNYLAGAWLNAGERAQ
                     RMARSAHPYIVPAQVFETSDGWLVVFISHDEFWRRFCGEVGRADWVQDARFATMAARR
                     ENRDAVLAALVPLFKGGSTASWMARLAPLGVVAAPVQTLEQALGSELAKERDMLVAID
                     AGGAAIRAVGNPIRVDGMAQAYAPPPQLGEHNAELLQARSSLADRVLARERRAIARAI
                     SEIENGSAEGAALGRQLAAHRGHARVIGITGPPGAGKSTLLAALTKELLAAGASVAVV
                     AVDPSSPVSGGALLGDRIRMGDVQGHEEVYIRSLASRGHAGGVSGATRSVVALLDAAR
                     FDYVLVETVGAGQSEVEIASIAATRLVVCPPGLGDDVQALKAGILEIADAFVVNKADL
                     AGAERTARDLLGMLALKKGPAVPVFSTAATTGQGVADVARWLQAPAARGGAVLAA"
     gene            complement(6889..7302)
                     /locus_tag="AC16O9_14160"
     CDS             complement(6889..7302)
                     /locus_tag="AC16O9_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR6517794.1"
                     /translation="MSAPAAGPLAGKRILVAKPGLDGHDAGAKVIALALRDAGAEVIY
                     TGLRKSPDFIVRVALDEDVAAVGLSILSGSHQALSCEVLEGLRAAGAGDIPVFVGGTI
                     PPQDREALLAAGVKGVFTNDAPLAQMIDELARALA"
     gene            complement(7299..8879)
                     /locus_tag="AC16O9_14165"
     CDS             complement(7299..8879)
                     /locus_tag="AC16O9_14165"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR6517795.1"
                     /translation="MSDARVTTPSGIEVPVVVREEMAAAPSAPGVYPYTRGIFADGYR
                     GRPWTIRQYSGFGSAETSNQRYRFLLEQGQTGLSVALDLPTQCGYDPDDPMARPEVGK
                     VGVSLFSLSEMETLFDGIDLGAISTSFTINGTAAIIYAMYLAAADKQGVPRAKLTGTI
                     QNDILKEYVARGTWIFPVRPSMRLIADSVLYANEVTPRFNAISIAGAHVRDAGCTAVE
                     EMAYTLANGLAYVDELIRRGGDVAKFARRLSFFFYVHMDLFEEACKFRAGRRLWAQLL
                     RSRYGVTDEKALHFRFGVVCGGSSLVAAQPYNNIVRVAIENLAAVLGGAQSIFTCAYD
                     EAFQIPTEFSAELALRTQQIVQHESGVAATVDPLGGSYYVEWLTDRMEEGMRQAMGEI
                     DAYGGVVKAIEDGWLQRRIALRAREKKDATDRGERVVVGMNRYRREGQAQSFGEVFRL
                     DPGTAARVLEKHAAVKQRRDAARAGRALDALEAAAADDKENLMPLLVECCHAYATVGE
                     MVARLKRCWGEFQEPVRL"
     gene            complement(8876..9613)
                     /locus_tag="AC16O9_14170"
     CDS             complement(8876..9613)
                     /locus_tag="AC16O9_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR6517796.1"
                     /translation="MSTVKSADRTLQILETFATAGEPLGIAELARRLAIPVSACYALL
                     RTLELRGYLYELGLRKGWYPTLRWLQKARAVAEHDPMLERVTPLLEALGRATGETIVF
                     GKRSGTEVVYLNVVESPHSIRYSAQPGDRKPLHSSAAGKALLGALPPAERAALLDTLK
                     LARVTRNTLVRRELLEKDLAAGAKRGWYATRGENVADVHALGAPVRIDGELYAVVVAG
                     PAHRIEAALKPLAEALQRTARAIGARR"
     gene            complement(9682..11082)
                     /locus_tag="AC16O9_14175"
     CDS             complement(9682..11082)
                     /locus_tag="AC16O9_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6517797.1"
                     /translation="MDIPYTPARQLARLLHARKLSATEVMQAFIARIERVNPKVNAIV
                     TFLPEQALKEAKALDRQKGKKPPLAGLPIAYKDLLPTKGVRTTMGSLVFKDNVPAADA
                     LLVERLKAAGAITLGKTNTPEFGAGSNTFNKVFGATRNPYDPSKTAGGSSGGAAAAVA
                     CGMLPFADGSDLAASLRNPGNYCNVVGFRPTPGRVPSYPAANAWDTQSVLGPVARTVE
                     DAAFLLSAMAGPDRRAPVSIAEPGSIFAKPLKRSFKKVRMAWTKDFGGLPVEPAVSAV
                     LEAQRRVFQDLGCIVEEACPDFSGATEAFETLRAVSFALRYARYLKTHRAELKATVIW
                     NIEQGLALDGAKIARAEVLRTELYQRLRAFLETYEFLLAPVSQLPPFPVETEYPTEIA
                     GVKLTNYLDWMKSCYYITIASHPAISVPAGFTPAGLPVGLQVIGRYRDDFGVLQLAHA
                     FEQETQAWKRRPPLAG"
     gene            11134..11790
                     /locus_tag="AC16O9_14180"
     CDS             11134..11790
                     /locus_tag="AC16O9_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517798.1"
                     /translation="MLLFFDIAPEAIAEHDDWHTHEHLPERLSIPGFLRGTRWVGLRG
                     GQSRYFVIYEVRDTGVLTSAAYLERLNNPTPWTQKMMPSYRGMTRGFCAVTGSAGLGM
                     GHAALVVRFKPGDAAPLRDWLVKEVIPGLATRPGLGGCFLFESAVAPPMTREQRIRGA
                     DAGVDWALVVTGYDEETVAGVERADLAKTRLEGRGAAIAGFGLYRTHYTLYEAEAKRS
                     "
     gene            complement(11794..13251)
                     /gene="rng"
                     /locus_tag="AC16O9_14185"
     CDS             complement(11794..13251)
                     /gene="rng"
                     /locus_tag="AC16O9_14185"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HIR6517799.1"
                     /translation="MSEEVLVNITPQETRVAAVHSGAVQELLIERAASRGLVGNIYMG
                     RVARVLPGMQSAFVEVGLERAAFRHVADIWEQRRADGEAAKPIEKVLAEGQPLLVQVV
                     KDPIGTKGARLSTQVSIAGRLLVYLPQDPHIGISQRIEDESGRQALRDQVRALVPPEE
                     KGGFIIRTLAEGASDAELAADIAFLRHLWADIRDRALGAQPPLLVHQDLSLAQRVLRD
                     MVTDATDVVRVDSRENFQKLAAFAETYTPQLRGRLEHYAGERPLFDLYNVEAEIEKAL
                     SRRVDLKSGGTLFIDQTEAMTTIDVNTGGYVGSRSFDDTVFKTNLEAAQAIARQLRLR
                     NLGGIIIVDFIDMQSDEHRAAVLEELKKALARDRTRMTVSGFTQLGLVEMTRKRTRES
                     LAHVLCEPCPVCDGRGAVKTARTVCYEILREILREARAFNAREFRVLGAQAVIDLLQE
                     EESGSVAMLQDFIGKPISLQVEPGYTPEQYDVVLM"
     gene            complement(13229..13867)
                     /locus_tag="AC16O9_14190"
     CDS             complement(13229..13867)
                     /locus_tag="AC16O9_14190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6517800.1"
                     /translation="MVKLLDRSIYLASRSPRRRELLGHIGVKFHLLLFRNRPGAAPDV
                     DEAPLAGENPRDYVMRVARDKAAAGWKRMLERNLPQAPVLAADTTVALEGRIFNKPAD
                     RAEAEMMLAALSGKRHEVLTAVALQYDDEVETALSVSDVQFRELSEEEIRAYVATGEC
                     DDKAGAYAIQGRAALFIAEIRGSQSGIIGLPLYETAQLLQKIGGRRERRSPR"
     gene            complement(13899..14297)
                     /locus_tag="AC16O9_14195"
     CDS             complement(13899..14297)
                     /locus_tag="AC16O9_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR6517801.1"
                     /translation="MARLLLVAHGARLRGWAAQARDDYLARMPRGYTVELAARAPANA
                     RIVALDERGRELTSVQFAAELARWAAEQAPTAFVVGGPDGLDARTKAGAALLLRLSSL
                     TLPHALAQVLLAEQLYRAATRLTGHPYHRE"
     gene            complement(14263..14637)
                     /gene="rsfS"
                     /locus_tag="AC16O9_14200"
     CDS             complement(14263..14637)
                     /gene="rsfS"
                     /locus_tag="AC16O9_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6517802.1"
                     /translation="MDIRRKQRAVVDALEDVKGRDILVFNVAQLSPFFERVVIASGDS
                     SRQLKALADSVRRKLAALGERVVGIEGEAGGEWVLVDLGDIVVHVMHPAVRSHYNLEE
                     IWGGKPVKLAGHGKAAPRRARR"
     gene            complement(14637..15182)
                     /gene="nadD"
                     /locus_tag="AC16O9_14205"
     CDS             complement(14637..15182)
                     /gene="nadD"
                     /locus_tag="AC16O9_14205"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIR6517803.1"
                     /translation="MSTTGILGGTFDPVHNAHLALARAALEHLALERIVWLPTGAPKY
                     RAPASTPAQHRVAMLRLALEGEPRYAIDERELDPAHSGYTVDTLKAIGGRPVLLLGAD
                     QYAKFETWHRWQEILERASLAVFARPGWAASDARARHVPFTPMNISASDIRARLARGA
                     DVSADVPPKVLEYIRRHGLYR"
     gene            complement(15182..>16379)
                     /locus_tag="AC16O9_14210"
     CDS             complement(15182..>16379)
                     /locus_tag="AC16O9_14210"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR6517804.1"
                     /translation="LARADTGAKNRALAAMAAALRADAAALLAANRDDVAHAQKDGRD
                     AAFVDRLTLTPALVEQMAAGLEQVAALPDPVGEVSGRVTRPTGIEVARMRVPLGVIGI
                     IYESRPNVTADAAGLCVKAGNACILRGGSEALHSNRAIAACVRAGLKAAGLPEAAVQV
                     VEVADRAAVGELITLHEYVDILVPRGGKDLIARLMRESRIPMLKHLDGVCHVYVDDRA
                     DPAMAVRIADNAKTQRYGTCNTMETLLVAEGIAPRVLPEIGRIFSAKGVEMRADDAAR
                     KLLPGAKPATEADFATEWLAPVVSIRVVKGVDEAIEHIARYGSQHTDAIVTADEARAQ
                     KFLREVDSSSVLVNASTRFADGFEFGLGAEIGISTDKLHARGPVGLEGLTTQKFIVLG
                     HGEIRR"
BASE COUNT         2459 a         5706 c         5771 g         2443 t
ORIGIN      
        1 ccaagggtcg tgggttcgaa tcccgccggg cgcgccacac ccacgccgat gtttgagctg
       61 agcgacacgc agaaggccct gcagcaaagc gcgcgcgacc tggcgaaaag gaagttcgcg
      121 ccgcgcgccg ccgagatcga ccgccgcgaa gaatacccgt ggcacaacgt cgagctgctg
      181 acgcaggccg gcttcatggg catgaccatt ccccaggagt acggcggcca gggactgggc
      241 tacctggagg cggtgctggt gatcgaggaa atggcgcggg tctgcggcgt cacgggccgc
      301 atcgtcgtcg aggccaacat gggggccatc ggcgccatca tgaagtacgg cacgcaggcg
      361 caaaagacgc tggccgcgaa gctggtgctg gccggggaca agccggcgat ctgcatcacc
      421 gaacccgagg cgggcagcgc ggcgacgcag atgagcacac gggccgatcg caagggcggg
      481 cgctacgtgc tgaacggccg caagcactgg atcaccggag gcggcgtctc caagctgcat
      541 ctgatcttcg cgcgggtgtt cgagaacggc gtggagcagg gcatcggcgg gttcattgcc
      601 gtgcgcggcg aggccagggg cctcgtcgtc ggccggcgcg agccggcgat gggcctgcgc
      661 ggcatcccgg agtgcgagat cctgttcgag gacctggaga taccgctgga gatgatgctg
      721 gtgccccccg agggaatccg gcgtggcttc gcaggactga tgaacgccta caacggccag
      781 cgcgtcggcg ccgccacggt ggcgctcggc atcgccgccg gggcctacga gctggcgctc
      841 gagtatgcgc agacgcgcaa gcagttcgac cgaccgatct gcgagtttca gggcctgcag
      901 tggattctgg tcgacatgca cacgcagctg gccgccgcgc gtctgctgat ccacaaggcc
      961 gcggcgggat ccggcgacgg ctttcccgac atgaccgagg ccgcccaggc caaggtgttc
     1021 gccgccgaca tggcgatcaa ggtcaccaac gacgccctgc aggttttcgg cgcggccggc
     1081 tattcgcgcg agttgccgct cgagcgcatg gtccgggacg cgcgcatgtt caccatcggc
     1141 ggcggcacgg cgcagatcct gcgcaccgtg attgcctcgc agctgctcgg acgcaagctg
     1201 ccgcagacgc gcgacggcta caggaatctc tagcttgaag gcacggttgc ccgcgcgccc
     1261 agccacgtaa tcgcggcgcc ggccgcggcg agcgccaacc cggcgagcga aagctgcagg
     1321 tggctcaggc ccagttcgcc gccgccgggc atcgccatga ccaggccgcc cgcgagcatg
     1381 agcagccgcg ccgcccagcc gagcgcgctg cgcgcaaccg cgccgatgcc caccacgtag
     1441 ccttcgagcg ccgagccgat gagcgcgatg ccggcgatcg ccgtgccgac cacgacgacg
     1501 atctcccacg gcgtgccgcg caggatgagc gccgggttga gcacgaagaa gaacggcacg
     1561 aaatacatcg ccgcgcccag gcgcaccgac tgcaggccga cctgcatggg cggcgccttg
     1621 gcgatgccgg cggcgacgaa cgcggcgagc gccaccggcg gcgtgatgaa cgacaccatg
     1681 ccccagtaca gcatgaacag gtgcaccgcc accggatcga agccggcgcc gaccagcggc
     1741 ggcgcgagca cgatggcgag gaagatgtag cacgcggtga tcgtcatgcc catgccgaag
     1801 atgaagctgg tgactgcgcc catcaccagc agcacgtaga tgttgttgcc ggcaaggtag
     1861 acgaggtcgt tggcgagcgt gccggcaagg ccggtgaccg agagcgcgcc gatgatgagg
     1921 cccacgcccg cgagcagcgc cgcgagctcg gaaagcgcgc cgcccacgcc ttctagcagc
     1981 gtgcgcaaac gcctccagtc gagccggtag cggcgcgaga actggttgat gaccaggagc
     2041 agcgccgtgg cgtagaacgg cgcgagcgac tctcgctgca gcacgagcaa catgtagacc
     2101 agcagcgcga acacgaacag gtagtgccag ccctcgagga atgtctgccc gagcgagggc
     2161 agctctgatc gcggcaagcc cttcaagccg ttcttcgccg cgtagccgtc gatctgcacg
     2221 aacagggcga agtagaaaag cagcgacggc accagcgcgg cgacggcgat ggcgacgtag
     2281 ggcaggccga ggaacgacgc catgacgaaa gcggtggcgc ccatcaccgg cggcatgagc
     2341 acgccgcccg tggaggcgca ggcctcgacc ccgcccgcgt aggccggccg gaagccgatg
     2401 cgcttcatcg ccgggatcgt gaccacgccg gtggtgagca cgttggaaac cacgctcccg
     2461 gagaccgagc ccatcaggcc gctggcgaag atcgacacct tggccgggcc gccgcgcacc
     2521 gagccgaaga gggcgaatgc gaggttgttg aagaaatgcg ccgcgccggt gtattgcagc
     2581 accgcgccga acatgacgaa gccgatgacg agctcgccga aggcgcgcgt cgggatgccg
     2641 agcgcactct cgctggaggc gaagtgaaat gacgcggtcg aggcaagatc gcgcggcagc
     2701 ccggcgatcg gccccggcat gtgcccggcg aacaccggat acagagagat cagcgccacg
     2761 accacgaaca cggcgttgcc gccggcgcgc cgcgtcgcct cgagcagcat cacccagccg
     2821 acgtaggcga tccaaaccgc ctgcgccggc gccttgtact cccatccttc ctcgacgatg
     2881 cgctgcgcgt gcagcgcgaa atacgcgaac acggcgaacg aggtcgcaaa cagcagcacg
     2941 tcgtaccagg gcacggcatt gcgccgcgcg tgcttgcccg ccggcaccag cagaaacacg
     3001 ctgcccacga gcagcgcgca tagccagtac agatacgcgg tgtcgagaag cgttttgccg
     3061 acgaagaagc cgaggttgag cagctggttg agcgcaaggc cgatcgcgcc cgagacgagg
     3121 acgatgagga cgacgcgcca cagcggcgcg agcgaacgat agcgtgcctc gatcggcgcc
     3181 gcctcggcgg cgtcggccat ggcgcttgcc taactcacca gctcgtctgc accgggtcga
     3241 agccggcctt ggtcagcgcc gcggcgcgcc gcttctgcca cgcttcgacg aacgccttgt
     3301 cgtcggcgta cgagcccttc ttctgctcgg cccaggccgc cgtgagcacc gtctggcgct
     3361 cgagaagctt gtcgttgtgc gcctgctcgg cggcgcccca cttgcccatt tccttccaga
     3421 agcggatcgc gccgtcgtgg aacgggatca cccaggcgaa gttctgccgg tcgaccgccc
     3481 agccggcgtt gcccggcgcg gcgcccttgt acatgtcgta ggtctcgacc atcgccttgg
     3541 tcatgttgta gacgagattc gcgtcctgcg aggcgtaggt catcaggatc gggtacggat
     3601 aggtggccga ttccgcgggc ttgtccttcg acagctccgc gccctcggtg cccatgaacg
     3661 ggaagaagaa cggcgccacc gcgttcatgc gcgcccagcc tgccttgtcc ttgtgcgaaa
     3721 tgatcgggta gacgaggccg cgcggcgact tggcgatctg ataggccttg cccgagatcg
     3781 acgaggtgaa ggcggcgtcg acctggttgt tgatgatgcc gtccatcgct gcaccgaagc
     3841 cgccgaactc gaccttgcgc acgtcgtccc aggtgaggcc ggagaaggcg agcatcgcgg
     3901 tgatgttctg gttgagcgcc ggcgagccga tcacccaggc gacgcgctta cccttcaggt
     3961 ccgcgatggt gcgcacgccc gcatcgcccg cagcgaccac ggtcagcacc tggtcggagg
     4021 tgttcagcat cagtccgcgc accggctgcg ggccccagct cggcgcgccg aactcgaaca
     4081 cgccttccat ggcgaggtag gctccgccca ctccgttggc ggagaattgc acctgaccct
     4141 cgcgaatcgg caggtttctc gacacgtcgt tcttgcccgg cagcaccctg aggctcacgt
     4201 tgtacttctg cttgagcgcg ttgccgatgg cgacggcctg gttgtagccg cccgagccga
     4261 cgtcgtaggc gctccaggaa atcgtcggcg gcagcttgac gtcggcgtgc gcggcgacgg
     4321 agaaagcgag cgtggcgccg agcgccgcgc gaagcaaagc gtgggtcatg atgccgtcct
     4381 cctccaaggt atgcccaccg atgttaaccg attccccggg gcgctagaat gctatccccg
     4441 atgagccagc cgatcagccg ctaccccgtt cccgagctca agaacctgcc cgaagacgtg
     4501 cgcgcgcgca tcctcgcggt gcaggaaaaa tcaggcttcg tccccaatgt gttcctggtg
     4561 ctggcgcacc gccccgagga gttccgggcg ttcttcgcct atcacgacgc gctgatggag
     4621 aaggagggtg ggctcaccaa ggcgcagcgc gagatgatcg tggtcgcgac ttccggggcc
     4681 aaccactgcc agtactgcgt ggtggcgcac ggcgccatcc tgcggatccg ggcgaagaat
     4741 cccctggtgg ccgaccaggt ggcgatcaac taccgcaagg ccgatatccc gccgcgagaa
     4801 aaagcgatgc tcgacttcgc catgaaggtg gcgctcgagt cgcacgcgct gggcgactcc
     4861 gacctcgagg cgctgcgccg acacgggttc accgacgagg acgcttggga catcgccgcc
     4921 atcgccgcct tcttcgcgct gtccaaccgg atggcggact tcaccgccat gcggccgaac
     4981 gacgagttct atccgatggg gcgggtgccg aaggagaagt agccgctcag gcggccagca
     5041 ccgcgccgcc gcgcgctgcg ggcgcctgca gccagcgcgc cacgtcggcg acaccctgcc
     5101 cggtggtggc ggcggtgctg aacacgggaa cggccgggcc tttcttcagc gcgagcatgc
     5161 cgagcagatc gcgcgccgtg cgctccgcgc ccgcgaggtc ggccttgttg acgacgaacg
     5221 cgtcggcgat ctccaggatg cccgctttca gcgcctgcac gtcgtcgccc aggcccggcg
     5281 ggcagaccac caggcgggtc gccgcgatgc tcgcgatctc gacctcggat tgccccgcgc
     5341 cgaccgtttc caccagcacg taatcgaagc gcgcggcgtc gagcagcgcg accacgctgc
     5401 gcgtggcgcc gctcacgccg ccagcgtgcc cgcgcgaggc gagcgagcgg atgtagacct
     5461 cctcgtgtcc ctgcacgtcg cccatgcgga tgcgatcgcc gagcagcgcg ccgcccgaca
     5521 ccgggctcga ggggtccacc gccaccaccg cgacgctcgc gccggccgcg agcagttcct
     5581 ttgtgagcgc ggcgagcagc gtggacttgc ccgcgccggg cgggccggtg atgccgatca
     5641 cccgggcgtg accgcgatgc gcggcaagct gccggccgag cgccgcgccc tcggcgctgc
     5701 cgttctcgat ttcgctgatg gcgcgcgcga ttgcgcggcg ctcgcgcgcc agcacgcggt
     5761 ctgcgaggct cgagcgcgcc tgcagcagct cggcgttgtg ctcgccgagc tgcggcgggg
     5821 gcgcataggc ctgcgccatg ccgtcgacgc ggatcgggtt gccgacggcg cggatcgctg
     5881 cgcccccggc gtcgatcgcg accagcatgt cgcgttcctt tgcgagctcg gagccgagcg
     5941 cctgctccaa cgtctggacc ggggcggcca cgacgccgag cggcgccagg cgcgccatcc
     6001 acgacgcggt gctgccgccc ttgaaaagcg gcaccagcgc ggcgagcacg gcgtcgcggt
     6061 tctcgcgccg cgccgccatg gtggcgaagc gcgcgtcctg cacccagtcg gcgcggccga
     6121 cctcgccgca gaaacgccgc cagaactcgt cgtgcgaaat gaacaccacc agccagccgt
     6181 cgctcgtctc gaacacctgc gccgggacga tgtacgggtg cgccgagcgc gccatgcgct
     6241 gcgcgcgctc gcccgcgttg agccacgccc ctgccaggta attcagctgc gagagcatta
     6301 cgtcgtgcat cgagacgtcg atctggccgc cgcggccttc gacgatcttc gatagcagcc
     6361 cgagcgcgcc catcatgccg gcggagttgt cgaccgccga gtagccggtc ttcgcgggcg
     6421 cgccgccggg ctcgccggtg atcgccatga cgcccgtgag cgcctggatg acgtagtcgt
     6481 aggccgggcg gtcggcgtgg ctgccttcca ggccgtagcc ggtgagcgca acgcaaacca
     6541 gcttcgggtt cgcgtctttc agcgcctcgt aggtcaggcc gagcttcctg atcgccgcgg
     6601 gccgcaggtt ggtgaccagg gcgtgcgcgc gggcggcgag gccgcgcagc gcggcgcggc
     6661 catcgtcgcg cgccaggtcg agcgcgacgc tcttcttgct gcggttgagg ctcgcgaaat
     6721 aggcgttatg cccgcccacc tggtgcgggc tgacgcgccg gccgatgtcg ccctcgggcg
     6781 gctcgatctt gatcacttcg gcgcccaggt cggcgagcag catgccgcag tagggaccgg
     6841 cgagcatgtg gccgacctcc agtacgacga tccccgagag cgggccgctc atgccagcgc
     6901 gcgcgccagc tcgtcgatca tctgcgcgag cggcgcgtcg ttggtgaaca cgcccttcac
     6961 gcctgcggcg aggagcgcct cgcggtcctg cggcgggatg gtgccgccga cgaataccgg
     7021 gatgtcgccc gcgcccgcgg cgcgcaggcc ctcgagcacc tcgcaggaca gcgcctggtg
     7081 gctccccgag aggatggaaa ggcccacggc ggcgacatcc tcgtcgagcg ccacgcgcac
     7141 gatgaaatcg ggcgacttgc gcaggcccgt gtagatcacc tccgccccgg cgtcgcgcag
     7201 cgcgagcgcg atcaccttgg cgcccgcgtc gtgcccatcg aggccgggct tggcgacgag
     7261 gatccgcttg cccgcgaggg gccctgcggc gggcgcgctc atagccgcac cggctcctgg
     7321 aactcgcccc agcagcgctt gaggcgcgcg accatctcgc ccacggtggc gtaggcgtgg
     7381 cagcactcga cgagcagcgg catcaggttc tccttgtcgt cggcggccgc ggcctcgagc
     7441 gcatcgagcg cccgccccgc gcgcgccgcg tcgcgccgct gcttcacggc ggcgtgcttt
     7501 tccagcacgc gcgcggccgt gcccgggtcg aggcggaaca cctcgccgaa gctctgcgcc
     7561 tgcccttcgc gccggtagcg gttcatgccg accaccacgc gctcgccgcg gtcggtcgcg
     7621 tccttcttct cgcgggcgcg caacgcgatg cggcgctgca gccagccgtc ctcgatcgcc
     7681 ttcaccacgc cgccgtaggc gtcgatctcg cccatcgcct ggcgcatgcc ttcctccatg
     7741 cggtcggtga gccactcgac gtagtagctg ccgccgagcg gatccacggt cgccgcgacg
     7801 ccgctttcgt gctgcacgat ctgctgcgtg cgcaatgcga gctcggcgga gaactcggtc
     7861 gggatctgga aggcctcgtc gtaggcgcag gtgaaaatcg actgcgcgcc gccgagcacc
     7921 gcggcgaggt tctcgatcgc cacgcgcacg atgttgttgt agggctgcgc ggcgacgagc
     7981 gaggagccgc cgcacaccac gccgaagcga aagtgcagcg ccttctcgtc ggtaacgccg
     8041 tagcggctgc gcagcagctg cgcccacagg cggcgcccgg cgcggaactt gcacgcctcc
     8101 tcgaacaggt ccatgtgcac gtagaagaag aacgacagcc gccgcgcgaa cttcgccacg
     8161 tcgccgcccc ggcggatgag ctcgtccacg taggcgaggc cgttggcgag cgtgtaggcc
     8221 atctcctcca ccgccgtgca gcccgcgtcg cgcacgtgcg ccccggcgat cgagatcgcg
     8281 ttgaagcgcg gcgtcacctc gttcgcgtag agcaccgagt cggcgatcag gcgcatcgac
     8341 ggccgcaccg ggaagatcca ggtgccgcgc gcgacgtact ccttcaggat gtcgttctgg
     8401 atggtgccgg tgagcttcgc gcgcggcacg ccctgcttgt cggccgcggc gaggtacatc
     8461 gcgtagatga tcgccgccgt gccgttgatg gtgaacgagg tggagatcgc gccgaggtcg
     8521 atgccgtcga acagggtctc catctccgag agcgagaaca gcgacacgcc caccttgccg
     8581 acctccggcc gcgccatggg gtcgtcgggg tcgtagccgc attgcgtggg caggtcgagc
     8641 gcgaccgaga gcccggtctg cccctgctcg agcaggaacc ggtagcgctg gttggacgtc
     8701 tccgcggagc cgaagcccga gtactggcgg atggtccagg gccgcccgcg gtagccgtcg
     8761 gcgaagatgc cgcgcgtata cggatagacg cccggcgcgc tgggcgcggc ggccatctcc
     8821 tcgcgcacca ccacgggcac ctcgattccc gagggcgtcg ttacgcgcgc gtcgctcatc
     8881 ggcgcgcccc gattgccctg gcggtgcgct gcagcgcctc ggcgaggggc ttgagcgcgg
     8941 cctcgatgcg gtgcgcgggt ccggcgacca ccacggcgta gagctcgccg tcgatgcgca
     9001 ccggggcgcc gagcgcgtgc acgtcggcga cgttctcgcc gcgcgtcgcg taccagccgc
     9061 gcttcgcgcc cgccgcgagg tccttttcca gcagctcgcg ccgcacgagc gtgttgcgcg
     9121 tgacgcgcgc gagcttgagc gtgtcgagga gcgcggcgcg ctcggccggc ggcagcgcgc
     9181 caagcagtgc cttgcccgcc gcgctcgagt gcagcggctt gcggtcgccg ggctgtgcgc
     9241 tgtagcggat cgagtgcggg gactcgacga cgttgaggta cacgacctcg gtgccgctcc
     9301 tcttgccgaa aacgatcgtc tcgccggtgg ccctgccgag cgcctcgaga agcggtgtta
     9361 cgcgctcgag catcggatcg tgctcggcca ccgcgcgcgc cttctgcagc cagcgcagcg
     9421 tggggtacca gcccttgcgc aggccgagct cgtacaggta cccgcgaagc tccagggtgc
     9481 gcagcagcgc gtagcaggca gacaccggga tcgcgagccg gcgcgcgagc tcggcgatgc
     9541 cgagcggctc gcccgcggtg gcgaaggtct ccaggatctg cagcgtgcgg tcggcgctct
     9601 tgacggtcga catgttcaca tatgtgaaat agctttcacg attgtggcag tgtgccagcg
     9661 cggcaaggcg cgggcaaggg gtcagcccgc cagcggcggg cggcgcttcc aggcctgcgt
     9721 ttcctgctcg aacgcgtgcg cgagctgcag caccccgaag tcatcgcgat agcgcccgat
     9781 gacctgcagg ccgacgggca gccccgccgg ggtgaagccc gcgggcaccg agatcgccgg
     9841 gtggctggcg atggtgatgt agtagcagct cttcatccag tcgagatagt tcgtcagctt
     9901 cacgccggcg atctcggtcg ggtactcggt ctcgaccggg aagggcggca gctggctcac
     9961 cggcgcgagc aggaactcgt aggtctccag gaacgcgcgc aggcgctggt agagctcggt
    10021 gcgcagcact tcggcgcgcg cgatcttcgc gccgtcgagc gcaagcccct gctcgatgtt
    10081 ccagatcacc gtggccttca gctcggcgcg gtgcgtcttc aagtaccgcg cgtagcgcag
    10141 ggcgaaggac accgcgcgca gcgtctcgaa ggcttccgtg gcgccgctga aatcggggca
    10201 ggcttcttcg acgatgcagc cgaggtcctg gaacaccctg cgctgcgctt ccagcactgc
    10261 gctcaccgcg ggctccaccg gcaagccgcc gaagtctttc gtccacgcca tgcgcacttt
    10321 cttgaaagag cgcttgagcg gcttggcgaa gatcgatccc ggctcggcga tcgacaccgg
    10381 cgcgcgccgg tcgggccccg ccatggcgga gagcaggaag gcggcgtctt ccaccgtgcg
    10441 cgcgaccggt ccgagcaccg actgggtgtc ccaggcgttc gccgcggggt aggaaggcac
    10501 gcggcccggc gtcgggcgga agccgacgac gttgcagtag ttgccggggt tccgcaggct
    10561 cgcggcgagg tccgagccgt cggcgaacgg cagcatgccg caagcgaccg cggccgcggc
    10621 gccgcccgag gagccgcccg cggtcttgga agggtcgtag ggattgcgcg tcgcgccgaa
    10681 caccttgttg aaggtgttgc tgccggcgcc gaattccggc gtgttggtct tgcccagcgt
    10741 gatcgcgccc gcagccttca ggcgctcgac cagcagcgcg tctgcagccg ggacgttgtc
    10801 cttgaagacg agcgatccca tggtggtgcg gacgcccttc gtcggcagca ggtccttgta
    10861 ggcgatgggc aggccggcaa gcggcggctt cttgcctttc tggcggtcca gggccttcgc
    10921 ttccttcagc gcctgctcgg gcaggaaggt gacgatcgcg ttcaccttgg ggttcacgcg
    10981 ctcgatccgg gcgatgaaag cctgcatcac ttctgtggcg gagagcttgc gcgcgtggag
    11041 aaggcgggcg agctggcgcg cgggcgtata ggggatgtcc atggcttcag tctatcgcgc
    11101 tagacttctc cggctatgct cggccgcgcc gccatgctgc tgttcttcga catcgccccg
    11161 gaggcgatcg ccgagcacga cgactggcat acgcacgagc acctgcccga gcgattgtcg
    11221 atcccgggat tcctgcgcgg cacgcgctgg gtgggcttgc gcggcgggca gtcgcggtat
    11281 ttcgtgatct atgaggtgcg cgacaccggc gtcctgacct ccgcggccta tctcgagcgg
    11341 ctcaacaacc ccacgccgtg gacccagaag atgatgccga gctatcgcgg gatgacgcgg
    11401 ggtttctgcg ccgtgaccgg gagcgccggg ctggggatgg gacacgccgc gctggtggtg
    11461 cggttcaagc ctggcgatgc ggcgccattg cgcgattggc tcgtgaaaga ggtcataccc
    11521 gggcttgcga cgcggccggg gctgggtggc tgcttcctgt tcgagtcggc cgtcgcgccg
    11581 ccgatgacgc gtgagcagcg cattcgtgga gcggacgcgg gcgtggattg ggcgctggtg
    11641 gtcaccgggt acgacgagga aacggttgcc ggtgtcgagc gcgctgattt ggcgaagact
    11701 cgactggaag gtcgcggggc ggcgatagcg ggtttcggcc tctaccgcac gcactacacg
    11761 ctttacgagg ccgaggcgaa gcgttcttga ggactacatc agcacgacgt cgtactgctc
    11821 cggcgtatac cccggctcga cctgcagcga gatcggcttg ccgatgaagt cctgcagcat
    11881 cgccaccgac cccgactcct cctcctgcag caggtcgatc accgcctgcg cgcccagcac
    11941 gcggaactcg cgcgcgttga aggcgcgcgc ctcgcgcagg atctcgcgca ggatctcgta
    12001 gcacacggtg cgcgcggtct tcaccgcgcc gcgcccgtcg cataccgggc agggctcgca
    12061 cagcacgtgc gcgagcgact cgcgcgtccg tttgcgcgtc atctccacga ggccgagctg
    12121 ggtgaagccg ctcaccgtca tcctcgtgcg gtcgcgcgcc agcgccttct tcagctcctc
    12181 cagcaccgcg gcgcgatgct cgtcggactg catgtcgatg aagtcgacga tgatgatgcc
    12241 gccgaggttc ctgaggcgca gctgccgcgc gatcgcctgc gccgcctcga ggttggtctt
    12301 gaacacggtg tcgtcgaaac tgcggctgcc gacgtagccg ccggtgttga cgtcgatcgt
    12361 ggtcatcgcc tcggtctggt cgatgaacag cgtcccgccc gatttcaggt cgacgcggcg
    12421 cgagagcgcc ttctcgatct ccgcctcgac gttgtacagg tcgaacagcg gccgctcgcc
    12481 ggcgtagtgc tcgagccggc cgcgcagctg cggcgtatag gtctcggcga acgccgcgag
    12541 cttctggaag ttctcgcgcg agtccacgcg caccacgtcg gtggcgtcgg tgaccatgtc
    12601 gcgcagcacg cgctgcgcga gcgacaggtc ctggtgcacg agcagcggcg gctgcgcacc
    12661 gagcgcgcgg tcgcggatat cggcccacag gtggcgcagg aacgcgatgt cggcggcgag
    12721 ctcggcgtcg ctcgcgccct cggcgagcgt ccggatgatg aagccgccct tttcctcggg
    12781 cggcaccagc gcgcgcacct ggtcccgcag cgcctggcgg ccgctctcgt cttcgatcct
    12841 ttgcgagatg ccgatgtgcg ggtcctgcgg caggtacacg agcagccgcc cggcgatcga
    12901 cacctgggtg gagagccgcg cgcccttggt gccgatcggg tccttcacta cctgcacgag
    12961 cagcggctgc ccctcggcca gcaccttctc gatgggcttg gccgcctcgc cgtcagcgcg
    13021 gcgctgctcc cagatgtcgg cgacgtgccg gaacgcggcg cgctccaggc ccacctcgac
    13081 gaacgccgac tgcatgccgg gcagcacgcg cgccacgcgt cccatgtaga tgttgcccac
    13141 cagcccgcgg ctcgccgcac gctcgatcag cagctcctgc accgcgccgg agtgcaccgc
    13201 cgcgacgcgc gtttcctgcg gcgtgatgtt aacgaggact tcttcgctca cgccgccctc
    13261 cgatcttctg cagcagctgc gcggtttcgt acagcggcag tccgatgatg cccgactggc
    13321 tgccgcggat ctcggcgatg aacagcgccg cccggccctg gatggcgtag gcacccgcct
    13381 tgtcgtcgca ctcgccggtg gcgacgtagg cgcggatttc ctcctccgag agctcgcgga
    13441 actgcacgtc cgagaccgag agcgcggtct cgacctcgtc gtcgtactgc agcgccaccg
    13501 cggtcagcac ctcgtggcgc ttgcccgaga gcgcagcgag catcatctcg gcctcggcgc
    13561 ggtcggcggg cttgttgaag atgcggccct ccagggccac ggtggtgtcg gccgcgagca
    13621 ccggcgcctg cggcaggttg cgctcgagca tgcgcttcca gcccgcggcc gccttgtcgc
    13681 gcgccacgcg catcacgtaa tcacgcgggt tctcgcccgc cagcggcgcc tcgtcgacgt
    13741 cgggcgcggc gcccgggcgg ttcctgaaca gcagcaggtg gaacttgacg ccgatgtggc
    13801 caaggagctc ccgccgccgc gggctgcgcg aggcaaggta gatgctgcga tcgagcagtt
    13861 tgaccatggc ggcgccgctt ccgccgagtg taacaaagct actctcggtg ataggggtgc
    13921 ccggtgaggc gggtcgcggc ccggtacagc tgctcggcga gcagcacctg cgcgagggcg
    13981 tgcggcagcg tgagcgacga caggcgcaat agcagcgcgg ctccggcctt tgtcctcgcg
    14041 tccagcccgt cggggccgcc gacgacgaaa gcggtcggcg cctgctcggc ggcccagcgc
    14101 gcgagctcgg cggcgaactg cacgctcgtc agctcgcgcc cgcgctcatc gagcgcgacg
    14161 atgcgcgcgt tcgcgggcgc tcttgcggcg agctcgaccg tgtagccgcg cggcatgcgc
    14221 gcgaggtagt cgtcgcgcgc ctgcgccgcc cagccgcgca gcctagcgcc gtgcgcgacg
    14281 aggagcagcc ttgccatggc ccgcgagctt caccggcttg ccgccccaga tctcctcgag
    14341 gttgtagtgg ctgcgcaccg ccgggtgcat gacgtgcacg acgatgtcgc ccagatccac
    14401 cagcacccac tcgccgccgg cttcgccctc gatgccgacc acgcgctcgc cgagggcggc
    14461 gagcttgcgg cgcacgctgt cggcgagcgc cttcagctgg cggctggagt cgccgctcgc
    14521 gatcaccacg cgctcgaaaa agggcgagag ctgcgccacg ttgaacacga ggatgtcgcg
    14581 ccctttcacg tcctcgaggg catcgaccac cgcgcgctgc ttcctgcgga tgtccatcag
    14641 cggtacaggc cgtggcggcg gatgtactcg agcaccttcg gcggcacgtc ggccgagacg
    14701 tccgcgccgc gcgcgaggcg cgcgcggata tcgctcgccg agatgttcat cggcgtgaac
    14761 ggcacgtgcc gcgcgcgggc gtcgctcgcc gcccagcccg gccgggcgaa caccgcgagg
    14821 ctcgcgcgct cgaggatctc ctgccagcga tgccaggtct cgaacttggc gtactggtcg
    14881 gcgccaagga gcagcaccgg tcggccgccg atcgccttca gcgtgtccac ggtgtagccg
    14941 gaatgcgccg ggtcgagctc gcgctcgtcg atggcgtagc gcggctcgcc ctcgagcgcg
    15001 aggcgcagca tggcgacgcg gtgctgcgcc ggggtgctgg ccggcgcgcg gtacttcggc
    15061 gcgccggtcg gcagccacac gatgcgctcg agcgcgaggt gctcgagcgc ggcgcgcgcc
    15121 agcgccaggt gcgcgttgtg taccggatcg aacgtgccgc caagaatccc cgtggtcgac
    15181 actaccggcg gatttctccg tggccgagga cgatgaactt ttgcgtggtg aggccctcga
    15241 gccccaccgg cccgcgcgcg tgcagcttgt cggtggagat gccgatctcg gcgccgaggc
    15301 cgaactcgaa gccgtcggca aagcgcgtcg aggcgttgac cagcaccgag ctcgagtcca
    15361 cctcgcgcag gaacttctgc gcgcgcgctt cgtccgcggt gacgatggcg tcggtgtgct
    15421 gcgagccgta ccttgcgatg tgctcgatgg cctcgtcgac gcccttgacc acgcggatcg
    15481 acaccaccgg cgcgagccac tcggtggcga aatcggcctc ggtcgcgggc ttcgcgccgg
    15541 gcaggagctt tcgcgcggcg tcgtcggcgc gcatctcgac gcctttcgcg ctgaagatgc
    15601 ggccgatctc gggcagcacg cgcggcgcga tgccctcggc caccagcagc gtctccatgg
    15661 tgttgcaggt gccgtagcgc tgcgtcttgg cgttgtcggc gattctcacc gccatcgccg
    15721 ggtcggcgcg atcgtccacg tagacgtggc acacgccgtc caggtgcttg agcatcggga
    15781 tgcgtgactc gcgcatcagg cgcgcgatca gatccttgcc gccgcgcggc acgaggatgt
    15841 ccacgtactc gtgcagcgtg atcagctcgc ccacggcggc gcggtcggcc acttccacga
    15901 cctgcaccgc cgcctcgggc aggcccgccg ccttcaggcc cgcgcgcacg cacgcggcga
    15961 tggcgcggtt ggagtgcagc gcttccgagc cgccgcgcag tatgcaggcg ttgccggcct
    16021 tcacgcacag cccggcggcg tccgcggtga cgttcgggcg cgactcgtag atgatgccga
    16081 tgacgccgag cggcacgcgc atgcgcgcca cttcgatgcc cgtggggcgc gtcacgcgcc
    16141 cggagacctc gcccaccggg tcgggcagcg ccgccacctg ctcgagcccg gccgccatct
    16201 gctcgacaag ggccggcgtc agcgtcagcc ggtcgacgaa cgccgcgtcg cgcccgtcct
    16261 tctgcgcgtg cgcgacgtca tcgcggttcg cggcgagcag cgccgccgcg tcggcgcgca
    16321 gcgccgcggc catcgccgcg agcgcgcggt tcttggcgcc ggtgtcggcg cgggcgagg
//
LOCUS       DBNHKE010000208         5666 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1413655, whole genome shotgun sequence.
ACCESSION   DBNHKE010000208 DBNHKE010000000
VERSION     DBNHKE010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1413655"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1710)
                     /locus_tag="AC16O9_14215"
     CDS             complement(118..1710)
                     /locus_tag="AC16O9_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6517805.1"
                     /translation="MSSLNILVIDDERAIRQTVVAALGKAGYVAAEAGCVAEAAAKLK
                     GGEFDVALCDIKMPDGSGIDLVRDSRAAGTNTVFIMITAFASLETAVQALRAGAADYI
                     IKPVRNEELLNRLAGIDTLRRLSDENRVLRQAIEDRAPRLYQCTSPAMLEVERLVRKV
                     AAVDSTVLITGESGTGKGVVARAIHGQSTRREGQFLAVNCSAIPEHLLESEFFGHTKG
                     AFTGAERARKGLFLQATEGTLFLDEISDLPLPLQAKLLNAIEDKEVRPVGSEQVRRID
                     TRIIAATNADLRERIGQGRFREDLFFRLSMFEIHIPPLRERPADLRGLIQFLLRGFKG
                     EGNALVPREIDPVAEEILLAHGWPGNVRELENVIRRACLLAENGHIGVSDLTSEIVAG
                     TGPRAAAGAAPAAEGALREQLRVVEADIIRREIEAAGGDRRLAAQRLGLGLSTLYRKL
                     EELERRGLAEPATLLPGVECARQRPGGRNEIVVPARGAVRGGARGAGARRLRAAARGS
                     RETGGGRAAGVSAAARDAALFL"
     gene            complement(1718..3241)
                     /locus_tag="AC16O9_14220"
     CDS             complement(1718..3241)
                     /locus_tag="AC16O9_14220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6517806.1"
                     /translation="MKIGLQAPDGAVLVHPDAAPGAASTPQARGLSLRAKGLVVLAIL
                     FVYILVVLGVIALQWHKSLSVGERIENLHLQETKAERLQSSLSHAIINLQDTLKSGNP
                     KDLDTDLVLVVSDTRQLEAHYPGLARHADGLASAVAGLEPNMPGAALRSLRGQLEAVR
                     AEANAISAQIHESKDALTATHFAQQGWIATVAAAMSVGGVVLLGALIVLFFGRLARDI
                     GKLEARAMDIVTGYDGAPLEVTRGDEVGGLMRAVNRTQSMLRLREKQLELARQQRFYQ
                     EKMAAIGSLAAGVAHEINNPIAAISGLAQDMSDHRQARLCSDARCQPEMILEQTGRIA
                     LISRQLAEMASMHSQQPDLLDLNGLVERTCNFLGYDKRFAGIDVVLDLDRNLPAVRSV
                     ADHLTQVLMNLMINAADALESTHGGARRIRVATRAEAAGAVLTVSDNGHGMDAALLSR
                     VFDESFTTKPLDKGRGLGLFLCKNLIKQNGGRIELESVLHAGTTARIVLPPREGAVA"
     gene            complement(3390..3704)
                     /locus_tag="AC16O9_14225"
     CDS             complement(3390..3704)
                     /locus_tag="AC16O9_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6517807.1"
                     /translation="MTRFDVSLIAVSLAVLAAAPSAQAADINKGRQIFNQHCAGCHGP
                     RGISVMPNAPHLADGTVLLRPDGEILASIRGGKNAMPAYAGILKDAEILDVIAYMRTL
                     RR"
     gene            complement(3763..4362)
                     /locus_tag="AC16O9_14230"
     CDS             complement(3763..4362)
                     /locus_tag="AC16O9_14230"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR6517808.1"
                     /translation="MRALRQPGARVGVIGLGVGTLAAYGRPGDVYRFYEIDPAVVQIA
                     RREFGCLEDSFARVEVVLGDARLQLEREPAQQFDVLAVDAFAGDAVPMHLLTHEALAV
                     HVRHVKPGGVIAFHVSNQFLDLPALVLRLAGARGLKAAHVLDEARSRGGFSSDWVLLT
                     TDAAFPDLPEIRAATVSARPGADQRPWSDDFNNLLQVPK"
     gene            4559..5497
                     /locus_tag="AC16O9_14235"
     CDS             4559..5497
                     /locus_tag="AC16O9_14235"
                     /inference="COORDINATES: protein motif:HMM:NF048240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6517809.1"
                     /translation="MAYAVWVGNAKSPEPPARAEPALAHAVSAEQLGTLSQQLEARLK
                     QSPDDGPGWAMLARSYVMLGRFEPAARAYAQAAQRMPNNADLLADYADALAMARGRTL
                     EGEPLAIVKRALELDPGHAKSLALAGSAEFEGQRYSAAVAYWERVVPLTDAHSEFGRS
                     IRRSIAQARELGKLPSTGAAGVPALPHPAPAIAAAPAAASGLAGTVKLAPALDSQVAP
                     GDALFIFARATEGERAPLAVVRAKAGDLPYAFRIDDSQALTPHGGLSGQRRVVLVARI
                     SRSGDAIAKPGDLQGSSKPVAPGTTGLEIVIDQVRQ"
     gene            complement(5475..>5666)
                     /locus_tag="AC16O9_14240"
     CDS             complement(5475..>5666)
                     /locus_tag="AC16O9_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517810.1"
                     /translation="YREALKDYNVAILLNSGYYRSYWGRAMVFEALGEEAAARNDYAR
                     ACALGLREVCAKAIAAPGR"
BASE COUNT          840 a         2025 c         1916 g          885 t
ORIGIN      
        1 accgcgtacg gcttgcgcag gccttcgctg ctcttcgggc cctcgcccgt gagcagctgg
       61 cgcagccgcg aattctcatc ctcccgaacc acgtcgccgc tgccgtacag gctgcgctca
      121 tagaaaaagc gcggcgtctc gggcggcggc ggaaacacca gcggcgcggc ctccaccggt
      181 ctctcgggag cctcgggcag cggcgcgcag gcggcgagcg ccagcgccgc gtgcaccgcc
      241 acgaaccgca ccgcgcgccg gcacgacgat ctcattgcgc ccgcctggtc gctgccgcgc
      301 acactcgaca cccggcagca gggtcgcggg ctcggcgagg ccgcgccgct cgagctcctc
      361 gagcttgcgg tacagcgtcg acaggccgag cccgagacgc tgggccgcca gccggcggtc
      421 gccgcccgca gcctcgattt cccgccgaat gatgtcggct tcgaccacgc gcagctgctc
      481 gcgcagcgcg ccctccgcgg ccggcgcggc gccggccgcg gcgcgaggcc ccgtgccggc
      541 gacgatttcc gaggtcaggt cgctcacgcc gatgtgcccg ttctccgcga gcaggcaggc
      601 gcgccggatg acgttttcga gttcgcggac gttgccgggc cagccgtgcg ccagcaggat
      661 ttcctcggcc accggatcga tctcgcgcgg cacgagtgcg ttgccctcgc ccttgaaacc
      721 ccgcagcaga aactggatca gcccgcgcag gtccgcggga cgctcgcgca gcggcgggat
      781 gtggatttcg aacatgctga ggcggaaaaa caggtcctcg cggaaccggc cctggccgat
      841 cctttccctg agatcggcgt tggtggcggc gatgatccgg gtatcgatgc ggcgcacctg
      901 ctcgctgccc accggccgca cctccttgtc ctcgatggcg ttgagcagct tggcttgcag
      961 gggcaatggc agatcgctga tctcgtcgag gaacagggtg ccctcggtcg cctgcaggaa
     1021 aagccccttg cgcgcccgct cggcaccggt gaacgcgccc ttggtgtggc cgaagaattc
     1081 gctttccagc agatgctccg gaatggcgct gcaattcacg gcgagaaact gcccctcgcg
     1141 ccgcgtgctc tgcccgtgga tcgcgcgtgc caccacgccc ttgccggtgc cgctctcgcc
     1201 cgtgatcagg accgtgctgt cgaccgcggc gaccttgcgc acgagccgct ccacttccag
     1261 catcgccggc gaggtgcact ggtagagcct cggcgcgcgg tcctcgatag cctggcgcag
     1321 cacccggttc tcgtcgctca gcctgcgcag cgtatcgatg cccgcgagcc ggttgagcag
     1381 ctcctcgttg cgcaccggct tgatgatgta gtcggcggcc ccggcgcgca gcgcctgcac
     1441 ggcggtctcc agcgaggcga acgccgtgat catgatgaag accgtattgg tgcccgcggc
     1501 gcggctgtcg cgcaccagat cgatgccgct gccgtccggc atcttgatgt cgcacagcgc
     1561 gacgtcgaac tcgccgccct tgagcttggc cgccgcctcg gcgacgcacc cggcctcggc
     1621 cgccacgtag cccgccttgc ccagcgccgc gacaaccgtc tgccggatgg cccgctcgtc
     1681 gtcgatcacg aggatgttca ggctcgacat ggtccgttca ggcgacggcc ccctcgcgcg
     1741 gcggcaagac gatgcgcgcc gtggtgccgg catgcagcac cgattccagc tcgatgcggc
     1801 cgccgttctg cttgatcagg ttcttgcaca ggaacaggcc gaggccgcgg cccttgtcga
     1861 gcggcttggt ggtgaacgat tcgtcgaaaa cccgcgacaa cagggcggcg tccatgccgt
     1921 gcccgttgtc gctgaccgtg agcaccgcgc cggcggcctc ggcgcgcgtc gccacgcgaa
     1981 tccggcgtgc gccgccgtgc gtgctctcca gtgcatcggc cgcgttgatc atcaggttca
     2041 tcaagacctg cgtcaggtga tccgcgaccg agcgcaccgc gggcagattg cggtcgaggt
     2101 cgagcaccac gtcgatgccc gcaaagcgct tgtcgtagcc caggaagttg caggtcctct
     2161 cgaccaggcc gttgagatcg agcaggtcgg gctgctgcga gtgcatcgag gccatctcgg
     2221 caagctggcg cgaaatcagc gcgatgcggc cggtctgctc gagtatcatc tcgggctggc
     2281 agcgcgcatc cgagcaaaga cgggcctgcc tgtgatcgct catgtcctgc gccaggccgc
     2341 tgatggcggc gatcggattg ttgatttcgt gcgccacccc ggcggcgagc gacccgatcg
     2401 ccgccatctt ttcctggtag aagcgctgct ggcgcgcgag ttccagctgc ttttcccgga
     2461 ggcgcagcat ggattgcgtg cggttcaccg cgcgcatcag gccgcccacc tcatcgccgc
     2521 gcgtgacctc gagcggcgca ccgtcgtagc ccgtgacgat atccatcgcg cgcgcttcga
     2581 gcttgccgat gtcgcgcgcc aggcggccga agaacagcac gatcagcgcg cccaacagca
     2641 cgacgccgcc cacgctcatt gcggccgcga ccgtcgcgat ccagccctgc tgcgcaaagt
     2701 gggttgccgt cagcgcatcc ttcgattcgt ggatctgcgc cgaaatggcg ttcgcttcgg
     2761 cgcgcaccgc ctccagctgc ccgcgcagcg agcgcaacgc cgcgcccggc atgttgggct
     2821 ccagtccggc gacagcgctg gccaggccat cggcgtgccg ggccagcccc gggtaatgcg
     2881 cttcgagctg ccgcgtatcc gacaccacca gcacgagatc cgtgtccagg tccttcggat
     2941 tgccggactt cagcgtatcc tgcagattga tgatggcgtg cgacaacgaa ctctgcagcc
     3001 gttcggcctt ggtctcctgc agatgaaggt tctcgatcct ctcgcccacg ctcagcgact
     3061 tgtgccattg cagcgcgatg acgccgagga cgaccaggat gtagacgaac agaatggcca
     3121 ggacgaccag gccctttgcg cgcagcgaca gcccccgagc ttgaggcgtg gaagcggcac
     3181 ccggtgccgc gtccgggtga accaggaccg cgccatccgg cgcctgcaat ccgatcttca
     3241 tcgctgccgt cgcgttgcca tggaggaaac ttttgctacc cgggcagttc cacagccgtc
     3301 atcttcgtgc gaacggaggg acggggtttg atgcagatca aggcctcgcc ccggccgcgc
     3361 gcgcgcgagc ccgaaacagg gcggcgaact cagcgccgca gggtgcgcat gtacgcgatc
     3421 acatcgagga tttccgcgtc cttgaggatg ccggcatagg caggcatggc gttcttgccg
     3481 ccgcggatcg acgccaggat ctcgccatcg ggccgcagca gcacggtgcc gtcggcaaga
     3541 tgcggtgcgt tcggcatcac gctgatgcct cgggggccgt ggcagcccgc gcaatgctgg
     3601 ttgaagatct gccggccctt gttgatgtca gcggcttgcg ccgacggcgc cgcggccagc
     3661 accgccagcg agacggcgat cagcgaaacg tcaaatctcg tcatgcgcgt tctccgagca
     3721 gcagcgccgg cggcgcttgg ttgatgccga tgttacaccc gctcatttcg gcacctgcaa
     3781 caggttgttg aaatcgtcgc tccacggccg ctggtccgcg cctggccgcg cggaaacggt
     3841 cgcggcacgg atctcgggca ggtcggggaa ggccgcgtcg gtcgtgagca gcacccagtc
     3901 gctgctgaac ccgccccggg accgagcctc gtcgagcaca tgggcggcct tcagcccgcg
     3961 cgccccggcg agccgcagca ccagcgcagg cagatcgaga aactggttgc tgacatggaa
     4021 cgcgatgacg ccacccggct tgacgtgccg aacatgcacg gcgagcgcct cgtgggtaag
     4081 gaggtgcatc ggcacggcgt cgccggcgaa cgcgtccacc gcgagcacgt cgaactgctg
     4141 agcgggctcg cgctcgagct gcaggcgcgc atcgccgagc acgacttcga ctcgcgcgaa
     4201 actgtcctcg aggcagccga actcgcgccg cgcgatctgc accaccgccg ggtcgatttc
     4261 atagaaccgg tacacgtcgc ccggcctgcc gtaggccgcg agcgtgccga cccccaggcc
     4321 gatgacgccg acgcgcgcgc cgggctgccg cagggcgcgc aaggtgcgcc cgatgcccga
     4381 ggtggtctgg tcataggtgc ccagggcgcg ccggctgccg tttgatctgc ctcaaccccg
     4441 gtttgccgcc ggtctgttat tgatcgccgg catgatgtct tcgtcgccgc cgcccgttcg
     4501 ccgccgcgcg ctgctctggg ccgctctcgc cgtgggaggg gccgtcgccc tggctgccgt
     4561 ggcgtacgcc gtctgggtcg gcaacgccaa gtcgcccgag ccaccggcgc gcgccgagcc
     4621 cgcgctggcg cacgcggtat cggcggagca gctcggcacg ctgtcccagc agctcgaagc
     4681 gcggctcaag caatcgcccg atgacggacc gggatgggcg atgctcgcgc gctcgtacgt
     4741 gatgctcggg cgcttcgagc cggcggcgcg cgcctacgcg caggccgcgc agcggatgcc
     4801 gaacaacgcc gacctgctcg ccgactacgc cgatgcgctg gcgatggcgc gcggccgcac
     4861 gctggagggc gagcccctcg cgatcgtcaa gcgcgcgctc gagctcgacc ccggccacgc
     4921 caagtcgctg gcgctggcgg gaagtgccga attcgaaggc cagcgctatt cggccgcggt
     4981 tgcctactgg gagcgcgtcg tgccgttgac ggacgcgcat tccgagttcg ggcgttcgat
     5041 ccggcgcagc atcgcccagg cgcgcgaact cggcaagctt ccctcgaccg gcgcggcggg
     5101 cgtgcccgcc ctgccccatc ctgcgccggc catcgcggcc gcgccggcgg ccgcctccgg
     5161 tctcgccggc acggtcaagc tcgcgccggc gctggactcg caagtcgcgc ccggggacgc
     5221 gctgttcatt ttcgcgcggg ccacggaggg cgagcgcgcg ccgctcgcgg tagtccgcgc
     5281 gaaagcaggc gatctgccgt acgcgtttcg catcgacgac agccaggcgc tgacaccgca
     5341 cgggggcctt tcgggacagc gccgcgtcgt cctcgtcgcg cgcatttcca ggtccggcga
     5401 cgcgatcgcc aagccggggg acctgcaggg cagcagcaag ccggtcgcgc ccggcacgac
     5461 gggcctcgaa atcgttatcg accaggtgcg gcaatagcct tggcacagac ttcgcggagg
     5521 cccagcgcgc acgcgcgcgc atagtcgttg cgggcggcgg cctcttcgcc taatgcctcg
     5581 aacaccatgg cgcgacccca atacgatctg tagtaaccgg aattgagcag gatggcgacg
     5641 ttgtagtctt tcagcgcctc ccgata
//
LOCUS       DBNHKE010000209         3457 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_196720, whole genome shotgun sequence.
ACCESSION   DBNHKE010000209 DBNHKE010000000
VERSION     DBNHKE010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_196720"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1130
                     /locus_tag="AC16O9_14245"
     CDS             159..1130
                     /locus_tag="AC16O9_14245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6517811.1"
                     /translation="MSEPDWAFPNELRPRAEDWDFDLERALGALVQLRVEVPEDAFTA
                     QILGTERLGNGVVIGEDGLILTIGYLITEASSVWITNAQGAVLAGHPLAYDQATGLGL
                     VMPLGRLGAPALARGSAARARVGEDVLIAGHGGRAHALKAQIFDKREFAGYWEYVLDE
                     ALFAAPAHPQWGGTALIGADGKLLGVGSLLVEEKKGEQSVQGNMFVPVDILEPILEDL
                     VKRGRARRAARPWLGMYTTEMGAKLVVAGLAAGGPADRAGVKLGDVVVAVAGDRPAGL
                     ADLFRRVWALGPAGAEVPLTIARSGKRQDLRLASVDRGDFLKKPQLH"
     gene            complement(1153..1662)
                     /locus_tag="AC16O9_14250"
     CDS             complement(1153..1662)
                     /locus_tag="AC16O9_14250"
                     /inference="COORDINATES: protein motif:HMM:NF017045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HIR6517812.1"
                     /translation="MIEIKAKLKTSRAAYKLEVKGRLRLPFALRQQRGAKVKLDSGEE
                     VLLNLPRGEVLRGGDLVTASDGRVVEVQAAAEKLLHASLPGAAELAQAAYRLGDRHVP
                     VEFRPDGLRVAADAAVERMLRELGAKLSEVEAPFEPVVPPHEREHAQGHGHRAHHDHG
                     HDHDHGHKH"
     gene            complement(1659..2609)
                     /locus_tag="AC16O9_14255"
     CDS             complement(1659..2609)
                     /locus_tag="AC16O9_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517813.1"
                     /translation="MSFRQQSVLSLGPDGFHRIAYTDWGDADNPHVVVCVHGLSRNCR
                     DFDFLAQALSGDYRVVCMDVVGRGESDWLEDKAGYTFSTYLADAAALVARVTTPVRSG
                     FLREMRARTVDWVGTSMGGLMGMMLAAKRGTPIGRLVMNDVGPFVPWNGLFRLKGHLG
                     RPHGFGTLQAAGEFLRGALAAFGPLTEAQWQHLIKHSVRQRAEDARWYLRFDPAIGQW
                     KAQSDPELPIGPEFLRGIDLWTVWNAVNGPTLVLRGAESEVLLASTVEEMRRRKPGTQ
                     SVEFAGVGHAPALFDKKQIDAVRGFLKQRRGRDRRERASE"
     gene            complement(2606..>3457)
                     /locus_tag="AC16O9_14260"
     CDS             complement(2606..>3457)
                     /locus_tag="AC16O9_14260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6517814.1"
                     /translation="VSQLLVDPGDRVVEVVPLWPNLQEIPKILNARVATVALEFSPEG
                     WRLDLRKLLDALKPGTKALYLNSPNNPTGWTISREDQRAVLEHCRRHGIWIFADDAYE
                     RLYFEGNHVAPSFLDLAEPGERVISTNTFSKSWLMTGWRLGWAVLPAALVADMGKLVE
                     YNTSCAPVFVQRAGLAAVTQGEPVIARTLERFRKARDFLVQELRAIPGVEVASPSGTM
                     YAFFRVRGMTDSLGFCKRLVAEHGLGLAPGAAFGPEGEGYVRWCFAADQAKLADGAAR
                     LRKALGA"
BASE COUNT          513 a         1257 c         1190 g          497 t
ORIGIN      
        1 ggcaagatcc gctacgccaa gatcaaggtc gaggaaggcg ccgagctggc cggcgagatc
       61 agcatgatgg agcgcggccc gcagggcgtg ctggtcaagc gaacggggac gtaaactgcg
      121 cacttgacgc gcggccgcgg gcacgcaaca gtgcccgcat gagcgagccc gactgggcct
      181 ttcccaacga gctgcggccg cgcgccgagg actgggactt cgatctcgag cgcgcgctcg
      241 gcgcgctggt gcagttgcgc gtggaggtgc ccgaggacgc cttcacggcg cagatcctcg
      301 gcaccgagcg cctgggcaac ggcgtggtca tcggcgagga cggcctgatt ctcaccatcg
      361 gctacctcat caccgaggcc agctccgtat ggatcaccaa cgcccagggc gcggtgctcg
      421 ccggacaccc gcttgcctac gaccaggcca ccggcttggg gctggtgatg ccgctcgggc
      481 ggctcggcgc gccggcgctc gcgcgcggct cggcggcgcg cgcgcgcgtc ggcgaagacg
      541 tgctcatcgc cggccatggc ggccgcgcgc atgcgctcaa ggctcagatc ttcgataagc
      601 gcgagttcgc cggctactgg gagtacgtgc tggacgaggc gctgttcgcc gcgcccgcgc
      661 acccgcagtg gggcggcacg gcgctgatcg gcgcggacgg caagctgctc ggcgtgggct
      721 cgctgctggt cgaggaaaag aaaggcgagc aaagcgtcca gggcaacatg ttcgtgccgg
      781 tggacatcct cgagccgatc ctcgaggacc tggtcaagcg cggccgcgcg cggcgcgccg
      841 cgcggccctg gctcggcatg tatacgaccg agatgggagc gaagctggtg gtggcggggc
      901 tcgccgccgg cggtccggcc gaccgcgccg gcgtcaagct cggcgacgtg gtggtggcgg
      961 tggcgggcga tcgaccggcc ggtctggccg acctctttcg ccgcgtctgg gcgctcgggc
     1021 cggcgggtgc cgaggtcccg ctcaccatcg cccgctcggg caagcgccag gacctgcgcc
     1081 tggcatcggt cgaccgcggc gacttcctga agaagccgca gctacactga gcctatcggc
     1141 cgagttgcgg cctcagtgct tgtggccatg gtcatgatca tggccatgat cgtgatgagc
     1201 gcggtgacca tggccctgcg cgtgctctcg ttcatgcggc ggcaccactg gctcgaacgg
     1261 cgcctcgacc tcgctcagct tcgcgcccaa ctcacgcagc atcctttcga ccgcggcgtc
     1321 ggcggcgacg cgcagcccgt ccggccggaa ctcgaccggc acgtgccggt cgccgagccg
     1381 ataggcggcc tgcgccagct cggccgcacc cggcagcgac gcgtgcagca gcttttccgc
     1441 ggcggcctgc acctcgacca cgcgcccgtc ggaagcggta accaggtccc cgccccgcag
     1501 cacctcgccg cgcggcaggt tcagcagcac ctcctcgccc gagtccagct tcaccttcgc
     1561 gccccgctgc tgtcgcaagg caaacggcag gcgcaaccgg cccttcacct cgagcttgta
     1621 cgcggcgcgc gaagtcttca gcttggcctt gatctcgatc attccgacgc cctctcccgc
     1681 cgatccctgc cgcgccgctg cttgaggaag ccgcgcaccg catcgatctg cttcttgtcg
     1741 aacagcgcgg gcgcatgccc gacgcccgcg aactccacgc tctgcgtgcc cggtttgcgc
     1801 cggcgcatct cttccaccgt cgaggcgagc agcacctcgg actccgcgcc gcgcagcacc
     1861 agcgtcgggc cgttcacggc gttccatacc gtccacaggt cgatgccgcg caggaactcc
     1921 ggcccgatcg gcagctccgg gtcggactgc gctttccact gtccgatcgc cgggtcgaag
     1981 cgcaggtacc agcgcgcatc ctccgcgcgc tggcgcacgc tatgcttgat gaggtgctgc
     2041 cactgcgcct cggtcagcgg cccgaaggcg gcgagcgccc cgcgcaggaa ttccccggcc
     2101 gcctgcaggg tgccgaagcc gtgcggccgg cccagatggc ctttcagccg gaatagtccg
     2161 ttccacggca cgaacgggcc gacgtcgttc atcaccaggc ggccgatcgg cgtgccgcgc
     2221 ttggccgcca gcatcatccc catcaacccg cccatcgaag tgccgaccca gtccacggtg
     2281 cgcgcgcgca tctcgcgcag gaagcccgag cgcaccgggg tcgtcacgcg cgccaccagt
     2341 gccgcggcgt ccgcgaggta cgtcgaaaag gtgtagccgg ccttgtcctc cagccagtcg
     2401 ctctcgccgc ggccgacgac gtccatgcac acgacgcggt agtcgccgct cagcgcctgc
     2461 gcaaggaagt cgaagtcgcg gcagttgcgc gacagcccgt gcacgcacac caccacgtgc
     2521 ggattgtccg cgtcgcccca gtcggtatag gcgatgcggt ggaacccgtc gggcccgagg
     2581 ctgaggacgc tctgctgccg gaagctcaag cgcccagcgc ctttcgcagc cgcgccgcgc
     2641 catccgcgag cttcgcctgg tctgcggcaa agcaccagcg cacatagccc tcgccttcgg
     2701 ggccgaaggc cgcgcccggc gccaggccga ggccgtgctc ggcgaccagc cgcttgcaaa
     2761 agccgaggct gtcggtcatg ccgcgcacgc ggaagaacgc gtacatggtg cccgaggggc
     2821 tcgccacctc gacgcccggg atcgcgcgca gttcctgcac caggaagtcg cgcgccttgc
     2881 ggaagcgctc gagcgtgcgc gcgatcaccg gctcgccttg cgtcaccgcg gccaggcccg
     2941 cgcgctgcac gaacaccggc gcgcaggagg tgttgtactc gacgagcttg cccatgtcgg
     3001 ctaccagcgc cgcaggcagc accgcccagc ccaggcgcca gccggtcatc agccagctct
     3061 tcgagaacgt attggtgctg atgacgcgct cgcccggctc ggcgaggtcg aggaacgagg
     3121 gcgccacgtg gttgccctcg aaatacaggc gctcgtaggc gtcgtcggcg aagatccaga
     3181 tgccgtggcg gcggcagtgc tcgagcacgg cgcgctggtc ctcgcgcgag atggtccagc
     3241 cggtggggtt gttgggcgag ttgaggtaga gcgctttcgt ccccggcttg agcgcatcga
     3301 ggagcttccg caggtccaga cgccagccct cgggcgagaa ctcgagcgcg acggtcgcga
     3361 cccgcgcgtt gaggatcttg gggatctcct gcaggttcgg ccacagcggc accacctcga
     3421 ccacgcggtc gcccggatcg accagcagct gcgacac
//
LOCUS       DBNHKE010000210         4377 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_38267, whole genome shotgun sequence.
ACCESSION   DBNHKE010000210 DBNHKE010000000
VERSION     DBNHKE010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_38267"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16O9_14265"
     CDS             <1..186
                     /locus_tag="AC16O9_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517815.1"
                     /translation="SDLAMRIATEAVQLHGGYGYCKDYRVERLMRDAKITQIWEGTNQ
                     IHRQLIGRSFIDKETHG"
     gene            179..1054
                     /locus_tag="AC16O9_14270"
     CDS             179..1054
                     /locus_tag="AC16O9_14270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517816.1"
                     /translation="MADMRTVGVAGCGTMGAGIAIVAARAGFRTLVYDTRAEALDRAR
                     QQTEAFFAKSVARGKLKAEQVTAILGNLVGTTKLEDFKACDVVIEAVFEDLKVKHGLF
                     AQLNGICPPHTLFASNTSTISITEIAGGSGRDDRFVGMHFCLPAQLMKLVEMSPGLNT
                     SEASFQAAWDFCKALGQHPVKTQDTPGFVLNYFLIPYHNDAIRLVEAGVAEPAEIDKA
                     IKGAMGYAMGPFELLDLVGMDTHLLVSEALQGLTHEPRAACPPLVKRMIAAGHLGRKS
                     GRGFHVYQDSKMYGA"
     gene            1057..1701
                     /locus_tag="AC16O9_14275"
     CDS             1057..1701
                     /locus_tag="AC16O9_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6517817.1"
                     /translation="MAYSLIDTGDSRSFPDAHALIQGAGAAEGEVVIVIGAAVARGLA
                     DIRDAESKTAILLELGTECLGVHTGESRGTEGSNVLGFARFRLGNAEPTKLVELVRQP
                     RTDPAALAAAKKVFEDAGLTVAVCNDFPGRIVERLIRPYLNAALRRLDEGLASADDMD
                     ATLRLGLGHPEGPIALLERTGLAAHFEVSQALYEALGDAAYAPARRARVAKQRA"
     gene            1702..2688
                     /locus_tag="AC16O9_14280"
     CDS             1702..2688
                     /locus_tag="AC16O9_14280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6517818.1"
                     /translation="MRAVVVREHGPLAQHRLETVADPVPGRGEVLIAARAIGVNYPDL
                     MVMQGSYQLLPPRPFSPGKEVAGVVGAVGAGVTRVRVGDPAMALPEYGTYAEKVVVPE
                     AHCFRMPEKMPFEEGAVLALAYQTAHFALLERGGYRKGESVLVTGASGGVGLAAVQIA
                     SALGATVLAGVTSREKGELCTRHGADHVVDLSLPDLRNSVREQVHAALGKRGVDIVLD
                     NVGGDAFDGALRALGWCGRLVSIGFASGRIPEAKAGYLLVKNLAVLGLQWTDYRAREP
                     GRVQQVQEALYALYGEGRLKPVITARYPLERFAEALARFEARQVQGKMLLLP"
     gene            2755..3507
                     /locus_tag="AC16O9_14285"
     CDS             2755..3507
                     /locus_tag="AC16O9_14285"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR6517819.1"
                     /translation="MDPDRDLPLALAALGAYFDAEGLACDYAALAASRERGHLAVCLA
                     ALETFDPRQVRIAAQMAFWINVFNAGVLRDAPELALELEAAEPGTLSFFDRPRLAIFG
                     LQFSLDDIYHGLLRGNAAAHGRLQAPMRRDDPRLAYTPIAFDERVHFALFRGSRSSPA
                     FCVFAGGSLDAQLERAATRYIRRLARVAPDGSAVTVPRLLQWYTRDFGDERDVLEFVL
                     NRLDEASLERAERNISRLKLRFDAYDWTLNRR"
     gene            complement(3494..3835)
                     /locus_tag="AC16O9_14290"
     CDS             complement(3494..3835)
                     /locus_tag="AC16O9_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6517820.1"
                     /translation="MKATLVIRRGRHGEPPREEVWEVPFEPGQSVLDALRWIRARRDP
                     TLAIRYSCTNANSCKECMVRIAGKTVYACIERLHEGTIRIEPLPNKRLVRDLVTEIAP
                     PDERLAGASGG"
     gene            complement(3832..>4377)
                     /locus_tag="AC16O9_14295"
     CDS             complement(3832..>4377)
                     /locus_tag="AC16O9_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6517821.1"
                     /translation="AVGGANGANRLSGNAITEALVFGRVAGESAARHVRPNAPGVDGA
                     VFAKEEQRLLAGHAACDFNPAAMLEEVQTLMQDDVGPFRTQSGLERALGRLRALRAEL
                     PAAKPAAGKPYDTALADWLDLCTMARVGECVAAAALARTESRGAHQREDFPGMDPQWA
                     FNQAVSLAGEDLRLESSAVPA"
BASE COUNT          642 a         1547 c         1538 g          650 t
ORIGIN      
        1 tccgacctag cgatgcgtat cgccactgaa gccgtgcagc tgcacggcgg ctacggctac
       61 tgcaaggact accgcgtgga gcgcctgatg cgcgacgcca agatcacgca gatctgggag
      121 ggcaccaacc agatccaccg ccagctcatc ggccgcagct tcatcgacaa ggaaacgcat
      181 ggctgacatg cgcacggtag gcgtcgcagg ctgcggcacc atgggggcgg gcatcgcgat
      241 cgtcgcggcg cgcgcgggct ttcgcacgct ggtctacgac acgcgcgccg aggcgctgga
      301 cagggcgcgc caacagaccg aggcgttctt cgccaagtcg gtcgcgcgcg gcaagctgaa
      361 ggcggagcag gtgacggcga tcctcggcaa tctcgtgggc accacgaaac tcgaggactt
      421 caaggcctgc gacgtcgtca tcgaggcggt gttcgaggac ctgaaggtga agcacgggct
      481 gttcgcgcaa ctgaacggca tctgcccgcc gcacacgctc tttgcctcga acacctcgac
      541 catctcgatc accgagatcg ccgggggctc cgggcgcgac gaccgtttcg tcggcatgca
      601 cttctgcctg ccggcgcagc tgatgaagct cgtggagatg tcgcccgggc tgaacaccag
      661 tgaagcgagc tttcaggcgg cgtgggactt ctgcaaggcg cttggccagc acccggtgaa
      721 gacgcaggac accccgggat tcgtgctcaa ttacttcctt attccgtacc acaacgacgc
      781 catccggctg gtcgaggcgg gcgtcgcgga gcctgccgag atcgacaaag ccatcaaggg
      841 cgcgatgggc tatgccatgg ggcccttcga gctcctcgac ctcgtcggca tggacacgca
      901 tctgctggtg agtgaggcgc tgcaggggct cacgcacgag ccgcgcgccg cctgcccgcc
      961 actcgtgaaa cgcatgatcg ccgccggcca cctgggccgg aaatccggcc gcggctttca
     1021 cgtctaccaa gactcgaaga tgtacggcgc ctgagcatgg cctattccct gatcgacacc
     1081 ggcgacagcc gttcctttcc cgacgcgcac gcactgatcc agggcgccgg ggcggccgaa
     1141 ggcgaggtgg tcatcgtcat cggcgcggcg gtggcgcgcg ggctcgcgga catccgcgac
     1201 gccgagagca agaccgccat cctgctcgag ctcggcaccg agtgcctggg cgtgcacacg
     1261 ggcgaatcgc gcggcaccga gggctccaac gtgctcggct tcgcgcgctt tcgcctcggc
     1321 aacgccgagc cgaccaagct cgtcgagctg gtccggcagc cgcggaccga tcctgccgcg
     1381 ctcgccgcgg cgaagaaggt cttcgaggac gccgggctca cggtggcggt atgcaacgac
     1441 tttccggggc gcatcgtcga gcgcctgatc cggccctacc tcaacgcggc gctccggcgc
     1501 cttgacgagg gcctcgccag cgccgacgac atggacgcga cgctcaggct cggactgggc
     1561 catcccgagg ggccgatcgc gctccttgag cgcacggggc tcgccgcgca cttcgaggtc
     1621 tcgcaggcgc tctacgaagc gctcggcgac gcggcctacg cgcccgcgcg ccgcgcgcgc
     1681 gtcgccaagc agcgcgcgta gatgcgcgcg gtcgtcgtcc gcgagcacgg cccgcttgcg
     1741 cagcaccggc tggaaaccgt cgcggatccg gtgccgggcc gcggcgaggt gctgatcgcg
     1801 gcgcgcgcga tcggcgtcaa ctaccccgac ctgatggtga tgcagggcag ctaccagctc
     1861 ctgccgccgc ggccgttcag cccgggcaag gaggtggccg gcgtggtggg cgcggtcggg
     1921 gcgggcgtga cgcgcgtacg cgtgggcgac cccgccatgg cgctgcccga gtacggcacc
     1981 tacgcggaaa aagtcgtggt gccggaggcg cactgcttcc gcatgcccga aaagatgccg
     2041 ttcgaggaag gcgcggtgct cgcgctcgcc taccagaccg cgcacttcgc gctcctcgag
     2101 cgcggcggct accgcaaagg cgaatcggtg ctcgtcaccg gggcgtccgg cggcgtgggg
     2161 ctcgccgccg tgcagattgc aagcgcgctg ggcgcgacag tgctcgccgg cgtcaccagc
     2221 cgcgaaaagg gcgagctctg cacgcgccac ggcgccgacc acgtcgtcga tctgtcactg
     2281 cccgatctgc gtaacagcgt gcgcgagcag gtgcacgcgg ccctgggcaa gcgcggcgtc
     2341 gacatcgtcc tcgacaacgt gggcggcgac gccttcgacg gcgcgctgcg cgcgctcggc
     2401 tggtgcggcc gcctggtgtc gatcggcttt gcctcgggcc gcattcccga ggcgaaggcg
     2461 ggctacctgc tggtgaagaa cctcgcggtg ctcgggctgc agtggaccga ctatcgcgcc
     2521 cgcgagcccg ggcgcgtgca gcaggtgcag gaagcgctct acgcgctcta cggcgagggc
     2581 cggctgaagc ccgtgatcac cgcgcgctat ccgctcgagc gcttcgccga ggcgctggcg
     2641 cggttcgagg cgcgccaggt gcagggcaag atgctgctcc tgccctgagc ggcgcctcat
     2701 ctgacgctcg tcgcggtcgc gccaaggatg ctgtaatagc ggtgggcagg agccatggac
     2761 cccgaccggg acctaccgct ggcactggcg gcgctgggcg cgtacttcga cgccgagggg
     2821 ctggcgtgcg actatgccgc gctcgccgcc tcgcgcgagc gcggccacct cgcggtgtgc
     2881 ctcgccgcgc tcgaaacctt cgacccccgg caggtgcgca tcgccgcgca gatggcgttc
     2941 tggatcaacg ttttcaacgc cggcgtgctg cgcgacgcgc cggagctggc gctggagctc
     3001 gaagccgccg agccgggcac gctgtcgttc ttcgatcgcc cgcggctggc gatcttcggt
     3061 ctgcagttct ctctcgacga catctaccac ggcctgctgc gcggcaacgc ggcggcgcac
     3121 ggccggctgc aagcgcccat gcggcgcgat gacccgcgcc tggcctatac gcccatcgcc
     3181 ttcgacgagc gggtgcactt cgcgctgttc cgcggctcac gctcctcgcc ggcgttttgc
     3241 gtcttcgcgg gcggcagcct ggacgcgcag ctcgagaggg ccgcgactcg ctacatccgg
     3301 cgcctggcgc gggtggcgcc cgacggcagc gcggtcaccg taccgaggtt gctgcagtgg
     3361 tacacgagag atttcggcga cgaacgcgac gtgctcgagt tcgtcctcaa ccggctggac
     3421 gaagcgtcgc tcgagcgggc cgagcgcaat atcagccgcc tgaagctgcg attcgacgcg
     3481 tacgactgga cgctcaaccg ccgctagcgc cggccagccg ctcgtccggc ggcgcgattt
     3541 cggtgaccag gtcgcgcacc agccgcttgt tcggcagcgg ctcgatgcgg atggtgccct
     3601 cgtgcagccg ctcgatgcac gcgtacacgg tcttgccggc gatgcgcacc atgcactcct
     3661 tgcagctgtt ggcgttggtg cacgagtaac ggatggcgag cgtcgggtcg cgccgcgcgc
     3721 ggatccagcg cagcgcatcg agcaccgact ggcccggctc gaacggcacc tcccagacct
     3781 cctcgcgcgg cggctcgccg tgcctgccgc ggcggatcac gagtgtggcc ttcatgcggg
     3841 caccgcgctc gactcgaggc gcaggtcttc gcctgcaagc gaaaccgcct ggttgaacgc
     3901 ccactgcggg tccatgccgg gaaagtcctc gcgctggtgc gcgccgcgcg attcggtgcg
     3961 cgcgagtgcc gccgcggcga cgcattcgcc gacgcgcgcc atggtgcaca gatccagcca
     4021 gtcggcgagc gccgtgtcgt agggcttgcc cgcggcgggc ttcgccgcgg gcagctcggc
     4081 gcgcagcgca cgcagccggc cgagcgcgcg ctcgagcccg ctttgggtgc ggaacggccc
     4141 gacgtcgtcc tgcatcagcg tctgaacctc ctccagcatc gcggccgggt tgaaatcgca
     4201 agccgcgtgg cccgcgagaa ggcgctgttc ctctttcgcg aaaactgctc cgtcgactcc
     4261 gggagcgttc gggcgcacgt gccgcgcggc gctttccccc gccacgcggc cgaacaccag
     4321 ggcctcggtg atcgcgttgc cggaaaggcg gttcgcgccg ttcgcgccgc ccaccgc
//
LOCUS       DBNHKE010000211         6336 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_990501, whole genome shotgun sequence.
ACCESSION   DBNHKE010000211 DBNHKE010000000
VERSION     DBNHKE010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_990501"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1704
                     /locus_tag="AC16O9_14300"
     CDS             199..1704
                     /locus_tag="AC16O9_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HIR6517822.1"
                     /translation="MARIAIGGFQHETNTFAPSPADYAAFEAGGGWPGVQYGEPLFGA
                     VAGANIPAAGAIDALRAAGHRLVGTAWAAASPSAAVTSDAYERIAGEMLRRLRDALPV
                     DGVYLDLHGAMVSEGFDDGEGELLRRVREAVGPRVPVVASLDLHCNFTRAMFAGADAF
                     VAYRTYPHVDMAETGARAARLLDSMLRGGARLAGAWRGFEYLTGIPSQCSFIEPCKGL
                     YADLAALEKKFDTSLSFTPGFPMADFAECGMAVQAYGTDAKRVEQAVQELRGRVADAE
                     KDFALDLHLPDEAVRRAGARGAPGRPVVLADTQDNPGAGGNGDTTGLLRALIGQRAEG
                     AALGLLIDKDAAAKAHAAGQGATLGFSLGGKSRIPGDAPFEGEFTVERLGDGKFTCTG
                     PMFKGFRMTLGSMALLRSTSAPGVRVVLASRKVQAADQEMFRHVGIEPVKERILALKS
                     SVHFRADFEPIAKEVLVVKAPGPALADPTEFKWTKLRKGVRLRPLGPAHAG"
     gene            complement(1709..2596)
                     /locus_tag="AC16O9_14305"
     CDS             complement(1709..2596)
                     /locus_tag="AC16O9_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6517823.1"
                     /translation="MKVFVTGASGYIGGSVATALRAAGHRVAGLVRSAQKAELVRALG
                     IEPVSGTLDDGALLARQAREADAVIHTADADHRASVEAMLAALAGSGKPFIHTSGSSI
                     VGDQAWGEASEKIYDDDTPFAPGPGRAARVAVGARVLAAAGEGVRAIVIAPSLIYGPG
                     RGAHRQSIQVPRLTALAKKSGIARHVGRGENIWANVHIDDLVELYLRALDKAPAGASY
                     WAENGECSMRALCEAISRALGYGGRTQPMTREESIVELGEGPAAYTYGSNSRVRATRA
                     RRELGWAPRHDSLLQSVAA"
     gene            complement(2593..3993)
                     /locus_tag="AC16O9_14310"
     CDS             complement(2593..3993)
                     /locus_tag="AC16O9_14310"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6517824.1"
                     /translation="MAAIHELSAAEIAASYEGGELSPVEATRALLDHIEAWEPRLNAM
                     YLIRRDAALKQARDAEGRWRVQAPLSPLDGVPVTIKENIHTKGDPAPIGTAANEPAAP
                     AGADAPPAARLRQAGCVILGKTTMPDYGMLSSGLSSVHGVTRNPWNTDRNTSGSSSGA
                     GAAAAAGYAPLHLGTDIGGSVRLPATHCGIFALKPSLGRVPVYPPFMGRVTGPMTRDV
                     EAAAAMMNWLALPDARDFMSLPYRPTDFTAELHGLDLKKLRIGLLPDMRAGLPVHAEV
                     RAAAQAAAKALAAAGAEVEEIPSFLTAEMLDGAARFFEARSHNDFAQLPPERQKKVLP
                     FIAEWCTWRAKDFSGRDVMQAYGQVMAMREAAVAASEPFDFVLSPVSPILPYEAELPC
                     PGNDPHNALPHIAFTVPYNMSEQPAASINWTFSADGLPIGVQLAGKRFDDVGVLKLAR
                     AIEDLRPTQRPWPALR"
     gene            4095..5405
                     /gene="ugpB"
                     /locus_tag="AC16O9_14315"
     CDS             4095..5405
                     /gene="ugpB"
                     /locus_tag="AC16O9_14315"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HIR6517825.1"
                     /translation="MKLRLLAAALAAAGAFAAPAQAQKEINWWHSMGGALGERTNEIA
                     AGFNASQSEWKVNAIYKGSYPESMTAAIAAFRAGNPPHIVQVFEVGTATMMAAKGAVK
                     PVYQLMKEANEPFDPKNYLGAVAGYYTDTKGNLLSMPFNSSTPVFYVNKDAFKKAGLD
                     AEKAPKTWKEFEAAAAKLKAAGQECVYTSSWPSWVHVENFSAWHNLPIGTKSNGFGGL
                     DTRFTINSPAHVKHVGMLGEFVKKGYFTYAGLGNKGDAKFASGECAMFTGSSGAQANI
                     KKLDKFAFSVNFLPYHDDVKGAPQNSIIGGATLWVLSQKDKSVYPGIAKFFTYLSSTE
                     VQAKWHQQTGYAPITNAAYDATKKAGFYEQNPGTDISVRQLSYKTPTANSKGLRFGNF
                     VQGRQVIEEEMEAVFAGKKDAKAAMDDAVKRGNDILIQFQKANK"
     gene            5454..6335
                     /gene="ugpA"
                     /locus_tag="AC16O9_14320"
     CDS             5454..6335
                     /gene="ugpA"
                     /locus_tag="AC16O9_14320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HIR6517826.1"
                     /translation="MEKRVVFRSAWLPYALLAPQIAVTIIFFFWPAVQAAWYSFQLQD
                     AFGLRTEFVGLSNFAVLFADEHYLASFKTTAVFSLWVAASGIVISLLLATMADRVLRG
                     ALAYKTLLIWPYAVAPAVAGVLWAFLFAPSIGILTYGLREIGVNWNWVLKGDQAMLLV
                     VIASVWKQVSYNFLFFLAGLQSIPRSLIEAAAIDGAGPLRRFWSVVFPLLSPTTFFLL
                     VVNIVYAFFDTFGVIDATTEGGPAGATQILVYKVYHDGVKASDLGGSSAQSVVLMMIV
                     IALTAVQFRFIERRVQY"
BASE COUNT          984 a         2118 c         2226 g         1008 t
ORIGIN      
        1 tggttgcggc tcatgtacgc cgtggtccag accggatcga ggatcgcgag gttcgagtgc
       61 ggtaccacgc gcagcacctt gtcctgggcg gagacggaga acgcggcgct ggccgcggtc
      121 agcaaaacaa aggcgaggat cttgcggttc atgcgggtat cctttgcgct cggattggca
      181 aaaacaggtt agcaggccat ggcacgcatc gcaatcggcg gtttccagca cgaaacgaac
      241 accttcgcgc cttcgccggc ggactacgcc gcgttcgagg cgggcggcgg ctggcccggc
      301 gtgcagtacg gcgagcccct gttcggggcg gtggccggcg ccaacattcc ggccgcgggc
      361 gcgatcgacg cgctgcgcgc cgcgggccac cggctcgtcg gcacggcctg ggcggccgcc
      421 agcccgtcgg cggcggtcac cagcgacgcg tacgagcgca ttgccggcga gatgttgagg
      481 cgcttgcgcg atgcgctgcc cgtggacggc gtctacctcg acctgcacgg ggccatggtg
      541 agcgagggct tcgacgacgg cgaaggcgaa ttgctgcggc gtgtgcgcga ggccgtgggc
      601 ccacgcgtgc cggtggtggc gagcctcgac ctgcactgca acttcacccg ggcgatgttc
      661 gcgggcgccg acgctttcgt cgcctaccgc acgtacccgc acgtcgacat ggccgaaacc
      721 ggggcgcgcg ctgcccgctt gctggacagc atgctccgcg gcggcgcgcg gctcgcgggc
      781 gcctggcgcg gcttcgagta cctgaccggg atcccctcgc aatgctcgtt catcgagccg
      841 tgcaagggcc tgtacgccga cctggcggcg ctggaaaaga agttcgatac gtcgctctcc
      901 ttcacgcccg gcttcccgat ggccgacttc gccgaatgcg gcatggcggt ccaggcctac
      961 ggcacggacg cgaaacgcgt ggagcaggcg gtgcaagagc tgcgcgggcg ggtcgccgac
     1021 gcggaaaagg acttcgcgct cgatctgcac ctgcccgacg aagcggtgcg gcgtgcgggc
     1081 gcgcgcggtg cccccggccg gccggtggtg ctcgccgaca cgcaggacaa tccgggcgcc
     1141 ggcggcaacg gcgacaccac gggactgctg cgcgcgctga tcgggcagcg cgccgaaggc
     1201 gcggcgctcg ggctgctcat cgacaaggac gccgcggcga aggcgcacgc ggcggggcag
     1261 ggcgccacgc tcggcttctc gctgggcgga aagtcgcgca tccccggcga tgcgcccttc
     1321 gaaggcgagt tcacggtcga gcgcctcggc gacggcaagt tcacctgcac cgggccgatg
     1381 ttcaagggct ttcgcatgac cctcggcagc atggcgctcc tgcgcagcac gtcggcgccc
     1441 ggggtgcgcg tggtgcttgc ctcgcgcaag gtgcaggccg cggaccagga gatgttccgc
     1501 cacgtcggca tcgagccggt aaaggagcgc atcctcgcgc tgaagagctc ggtgcatttc
     1561 cgcgccgact tcgagccgat cgcgaaggaa gtgctggtgg tgaaggcgcc ggggccggcg
     1621 cttgccgacc cgacggagtt caagtggacc aagctcagga agggcgtgcg cctgcggccg
     1681 ctcgggcccg cgcacgcggg ctgatcgtct aggcggcgac cgactgcagc agcgagtcgt
     1741 gccgcggcgc ccagccgagc tcgcgccgcg cgcgcgtcgc gcgaacgcgc gaattggagc
     1801 cgtaggtata ggcggcgggg ccttcgccca actccacgat cgactcctcc cgcgtcatcg
     1861 gctgcgtgcg cccgccgtag cccagcgcgc ggctgatcgc ttcgcatagc gcgcgcatcg
     1921 agcactcgcc gttctccgcc cagtacgacg cgccggccgg ggccttgtcg agcgcacgca
     1981 ggtacagctc gacgaggtcg tcgatgtgca cgttcgccca gatgttctcg ccgcgcccca
     2041 cgtggcgcgc gatgccggat ttcttcgcca gcgccgtgag ccgcggaacc tggatgctct
     2101 gcctgtgtgc gccgcgcccc ggcccgtaga tcaggctcgg ggcaatgacg atcgcgcgta
     2161 cgccttcgcc ggcggctgcc agcacgcgcg cgccgacggc gacgcgcgcc gcgcgtccgg
     2221 gtccgggcgc aaacggcgtg tcgtcgtcgt agatcttctc gctcgcctcg ccccaggcct
     2281 ggtcgccgac gatgctcgag ccgctggtgt ggatgaacgg cttgccgctg cccgcgagcg
     2341 ccgccagcat cgcttcgacc gaagcgcggt ggtcggcgtc ggcggtgtgg atcacggcat
     2401 cggcttcgcg cgcctggcgt gcgagcagcg cgccgtcgtc cagcgtgccg gataccggct
     2461 cgatgccgag cgcgcgcacc agctcggctt tctgcgcgct gcgcaccagc ccggcgaccc
     2521 ggtgtccagc ggcgcgcagg gccgtcgcca ccgagccgcc gatgtagccg gaagcgccgg
     2581 tgacgaaaac cttcatcgca gcgcgggcca gggcctctgc gtcgggcgca ggtcctcgat
     2641 ggcgcgcgca agcttaagca cgccgacatc gtcgaagcgc ttgccagcga gctgcacgcc
     2701 gatcggcagc ccgtcggcgc tgaaggtcca gttgatcgat gctgcgggtt gctccgacat
     2761 gttgtagggc acggtgaacg cgatgtgcgg cagcgcgttg tgcggatcgt tgcccgggca
     2821 cggcagctcg gcctcgtagg gcaggatcgg cgacacgggc gagagcacga aatcgaacgg
     2881 ctcgctggcc gccaccgcgg cttcacgcat cgccatcacc tggccgtagg cctgcatcac
     2941 gtcgcgtccg gagaaatcct tcgcgcgcca ggtgcaccac tcggcgatga acggcagcac
     3001 cttcttctgc cgctcgggcg gcagctgcgc gaaatcgttg tgcgagcgtg cctcgaagaa
     3061 gcgcgcggcg ccgtcgagca tctcggccgt gagaaagctc gggatctcct cgacctcggc
     3121 gcccgccgca gcaagcgcct tggccgcggc ctgcgccgcg gcgcgcacct ccgcgtgcac
     3181 cggaagcccg gcgcgcatgt cgggcagcaa gccgattcgc agcttcttca ggtcgagccc
     3241 gtggagttcc gccgtgaagt cggtcggccg gtagggcagg ctcatgaagt cgcgcgcatc
     3301 gggcagcgcg agccagttca tcatcgccgc cgcggcttcg acgtcgcgcg tcatcgggcc
     3361 ggtgacgcgg cccatgaagg gcgggtacac cggcacgcgc ccgaggctgg gcttgagcgc
     3421 gaagatgccg cagtgcgtcg ccggcagccg taccgagccg ccgatgtcgg tgccaagatg
     3481 cagcggcgcg tagcccgccg cggcggcggc gcccgcgccg gagctcgagc ccgaggtatt
     3541 tctgtcggtg ttccacgggt tgcgcgtgac gccgtgcacg ctcgacaggc ccgaggagag
     3601 catcccgtag tcgggcatgg tggtcttgcc caggatcacg cagcccgcct ggcgcaggcg
     3661 cgccgcgggc ggcgcgtcgg cgccggccgg tgcggccggc tcgttggccg cggtgccgat
     3721 cggcgccgga tcgcccttgg tgtggatgtt ctccttgatc gtcaccggca cgccgtctag
     3781 cggcgacagc ggcgcctgca cgcgccagcg cccttcggcg tcgcgcgcct gcttcagcgc
     3841 cgcgtcgcgg cggatcaggt acatggcgtt caggcgcggc tcccaggcct cgatgtggtc
     3901 gaggagcgcc cgcgtcgctt cgaccgggga gagctcgccg ccctcgtagc tggcggcgat
     3961 ttcggcggcg ctgagttcgt ggatggcggc catggcgttc gagttatata taccgtacgc
     4021 gaggataatt ctgtagtatt tccgtcatgt ggcggcgaca gcatcgccac tttcacagga
     4081 taagaggaga ttccatgaaa ctcagattgc ttgccgcggc attggccgcc gccggcgcgt
     4141 tcgcggcgcc ggcgcaggcg cagaaggaaa tcaactggtg gcattcgatg ggcggcgcgc
     4201 tcggcgagcg caccaacgag atcgcggccg ggttcaatgc cagccaatcg gagtggaaag
     4261 tcaacgcaat ctacaagggc agctatccgg agtcgatgac cgccgcgatc gcggccttcc
     4321 gcgccggcaa cccgccgcac atcgtccagg tgttcgaggt cggcacggcg accatgatgg
     4381 ccgccaaagg cgccgtcaaa cccgtctacc agctgatgaa agaggccaac gagcctttcg
     4441 atcccaagaa ctacctgggc gcggtcgcgg gctactacac cgacaccaag ggcaacctgc
     4501 tgtcgatgcc gttcaacagc tcgacgccgg tgttctacgt gaacaaggac gcgttcaaga
     4561 aagcgggcct ggatgccgag aaggcgccca agacatggaa ggagttcgag gccgcggcag
     4621 ccaagctgaa ggcggcgggc caggagtgcg tgtacacgtc gagctggccg tcctgggtcc
     4681 acgtggagaa cttcagcgcc tggcacaacc tgcccatcgg caccaagtcg aacggcttcg
     4741 gcgggctgga cacgcgcttc acgatcaatt cgccggcgca cgtgaagcac gtgggcatgc
     4801 tcggcgagtt cgtgaagaag ggctacttta cctacgccgg cctcggcaac aagggtgacg
     4861 ccaagttcgc gagcggcgaa tgcgccatgt tcaccggcag ctcgggcgcg caggccaaca
     4921 tcaagaagct ggacaagttc gcgttctcag tgaatttcct gccgtaccac gacgatgtca
     4981 agggtgcgcc gcagaactcg atcatcggcg gtgccaccct gtgggtgctg tcgcagaagg
     5041 acaagagcgt gtacccgggc atcgccaagt tcttcaccta cctctcgtcg accgaggttc
     5101 aggcgaagtg gcatcagcag accggctacg cgcccatcac caatgccgcg tacgatgcga
     5161 ccaagaaggc ggggttttac gagcagaacc cgggcacgga tatttcggtg cgccagctca
     5221 gctacaagac gccgaccgcc aactcgaaag gcctgcgctt cggcaacttc gtgcaaggcc
     5281 ggcaggtgat cgaggaggag atggaggccg tgttcgccgg caagaaggac gccaaggccg
     5341 cgatggacga cgcggtcaag cgcggcaacg acatcctgat ccagttccag aaggccaaca
     5401 agtagagctc ggggaagcaa aggggcgctg cggcgcccct ttgaattcct gccatggaaa
     5461 aacgggtcgt cttccgctcg gcgtggctgc cgtacgcgct gctcgcgccg cagatcgcgg
     5521 tcacgatcat cttcttcttc tggccggcgg tgcaggcggc ctggtactcg ttccagctgc
     5581 aggacgcctt cgggctgagg accgagttcg tcggcctcag caatttcgcc gtcctgttcg
     5641 ccgacgagca ctacctggcg tcgttcaaga ccactgccgt gttcagcctc tgggtggcgg
     5701 cctcgggcat cgtcatctcg ctgctgctgg cgacgatggc ggaccgcgtg ctgcgcggcg
     5761 cgctcgccta caagacgctg ctgatctggc cctatgcggt cgcgccggcg gtggccggcg
     5821 tgctgtgggc cttcctgttc gcgccgtcga tcggcatcct cacctacggt ctgcgcgaga
     5881 tcggcgtcaa ctggaactgg gtgctgaaag gcgatcaggc gatgctgctg gtggtgatcg
     5941 cctcggtctg gaagcaggtc agctacaact tcctgttctt cctcgccggc ctgcagtcga
     6001 tcccgcgctc gcttatcgag gcggcggcga tagacggcgc cgggccgctg cggcgcttct
     6061 ggagcgtggt cttcccgctg ctctcgccaa ccacgttctt cctgctggtg gtcaacatcg
     6121 tctacgcgtt cttcgacacc ttcggcgtca tcgacgcgac caccgagggc gggccggccg
     6181 gcgcgacgca gattctcgtc tacaaggtgt accacgacgg cgtcaaggcc tccgatcttg
     6241 gcggctcgtc ggcgcaatcg gtggtgctga tgatgatcgt gatcgcgctg accgcggtgc
     6301 aattccgctt tatcgagcgc cgggtgcagt actagg
//
LOCUS       DBNHKE010000212        36148 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1361602, whole genome shotgun sequence.
ACCESSION   DBNHKE010000212 DBNHKE010000000
VERSION     DBNHKE010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 36148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 36148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1361602"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC16O9_14325"
     CDS             complement(<1..917)
                     /locus_tag="AC16O9_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6517827.1"
                     /translation="MRLTLALLIALAPAWAHAQGKPAPAMPVRAALAKSAPAVDEATA
                     VGTLRADEAVTLRPEIAGRIAEIRFKEGQRVERGAVLVQLDQAEYAAVLASSRAQAEL
                     DRQRLERAADLFKKNFISQQAYDEARANAARSAAKQREDEARLAKTVIRAPFAGVAGL
                     RQVSEGAYVAAGTDIARLEKIDQLKLDFRVPEIYVGRLKPGQAVRVQLDAYGERSFPG
                     SVYALEPAVDEATRTVLVRARVANPQIKLRPGMFARVWAQLAVRENAVWVPEQAIVPR
                     GQDRFVFRVVDGKAEMVKVQTGVRKVGEV"
     gene            complement(960..1376)
                     /locus_tag="AC16O9_14330"
     CDS             complement(960..1376)
                     /locus_tag="AC16O9_14330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6517828.1"
                     /translation="MKPSLAPGAKGERTLTVDVARCIAFMGPENAVYATPHMVSDVEY
                     SIRDFLLEHLDAGEDTVGTRVVIDHLAATPAGMEVTLRYEVVAVEGRSVRCAFTVHDN
                     VEQCGRGEHVRFVVDVEKTRQRLAKKRASLPGHPAP"
     gene            complement(1373..1579)
                     /locus_tag="AC16O9_14335"
     CDS             complement(1373..1579)
                     /locus_tag="AC16O9_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6517829.1"
                     /translation="METVRLGIEGMTCAGCVNSVTRVLTALPGVAQADVSLTKARAKV
                     TYDPAHTSVEAIKQAIERAGYKAA"
     gene            complement(1627..2055)
                     /locus_tag="AC16O9_14340"
     CDS             complement(1627..2055)
                     /locus_tag="AC16O9_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6517830.1"
                     /translation="MKILVAVDGSKASLEAVDWLVAQANQFREPPTVELLTVHAPIPK
                     LVRVGLRMKKAEIAKWYRGQGAANLKQAERRLQRARLKYHAQVHVGPVAETIVRHAQR
                     TKCDLIVIGSSGMGAAANLLAASVATKVLHLAKRPVLLAR"
     gene            2164..3312
                     /locus_tag="AC16O9_14345"
     CDS             2164..3312
                     /locus_tag="AC16O9_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HIR6517831.1"
                     /translation="MLIPLEAPSQPVDPARFALFDLGFRPFYLLAGAYAALAIPLWGL
                     EYAGWLPGPGILWHAHEMVFGYAFAVIAGFLLTAVRAWTGRPTPSGAALAALAGLWLA
                     ARALAYVSLPLAAWAGALFALALAFAIGRPLVAAGNRRNLFFIALVLGLGAAGIVVQR
                     HPQLGLALGLDAVLFIMAVMAGRVVPAFTNNAVAGAGARRLPWLEWSSLGALLALLAA
                     DTLQRPELAAGLALLAAPLHAARLALWAPGKVLRRPILWILHLSYAWIVVHLLLRGFT
                     GFGFLPYALGTHALTVGAIGGLTLGMMTRTARGHTARPLQAGRAEVAAYLLVQLAAVT
                     RVLVPLAVPAAYVGAALAAALFWFAAFAVFTLAYVPILTRPRLDGQPG"
     gene            complement(3314..3706)
                     /locus_tag="AC16O9_14350"
     CDS             complement(3314..3706)
                     /locus_tag="AC16O9_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR6517832.1"
                     /translation="MSAAELYPYLRTLHIACAAISIAGFLARGALMLMGSASLQMRFV
                     RVAPHVVDTVLLASALWLAWFVGQVPLVHDWITAKVLVLVAYIALGTIALKRGRTKRV
                     RAAAFAAALASAGYIVWVALTRDATPWS"
     gene            complement(3693..4103)
                     /locus_tag="AC16O9_14355"
     CDS             complement(3693..4103)
                     /locus_tag="AC16O9_14355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIR6517833.1"
                     /translation="MRLQKNTLLALYSALEFAARPGRQLSVTEIAAALHVSPHHLAKV
                     LRMLGRAGLVDSVRGARGGYRFVGNAKRVTLMDVVALFEDIGSAVPRGRRARRGAEQA
                     LDGVLAEIDQTARATFGSVTLDTMLKLIARHERG"
     gene            complement(4100..4525)
                     /locus_tag="AC16O9_14360"
     CDS             complement(4100..4525)
                     /locus_tag="AC16O9_14360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6517834.1"
                     /translation="MRAAASADLPQVIKIDQRATGLAKPEYWAGILRRYGRGGVRRRF
                     LVAEAGGDILGYIVGEVRDWEFGSPPCGWVFGLGVRPQAREAGVATAMLKAITAAFGR
                     QGVATVRTLIARDNRLVLSFFRSQGMMAAPVIPLEKDIG"
     gene            4659..5795
                     /locus_tag="AC16O9_14365"
     CDS             4659..5795
                     /locus_tag="AC16O9_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6517835.1"
                     /translation="MKLHAWFAAALLAAASATQAQAPVRIGAFLSVTGPAAFLGDPEQ
                     KTLELYVERLNAAGGVLGRKLELVAYDSGGDAEKARAFAKRLIEQDKVDVIVGGSTTG
                     ETMAVVPLAEAAGMPFISLAGAVVIVEPVKKWVFKTPHTDRMACDKIFVDMKARGVAK
                     AALISGSGGFDKSMRAQCLGVAKNHGIDIVADETYGAGDTDMTAQLTKIKNATGVQAV
                     LNAGFGQGPAIVTKNYKQLGIGVPLYQSHGVASKQYIQLAGDAAEGVRLPAAALLVAE
                     ALPDSDPQKKVVLEYKRAYEERFKQDVSTFGGHAYDGLMLAVEAMKKAGGTDKAKVRD
                     ALESIRGYLGTAGVVNMSPQDHMGLDLTAFRVLEVRKGDWTLVK"
     gene            5799..6674
                     /locus_tag="AC16O9_14370"
     CDS             5799..6674
                     /locus_tag="AC16O9_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517836.1"
                     /translation="MSGELAQFLLSGLTVGAIYALVALGFSIIYNASHVINFAQGEFV
                     MIGGMATVSLLGAGLPLAPAALAAVALAALLGLLLARFAVQPARGASVVTLIIITIGA
                     AILLRGLASIVWDKRIHALPAFSGEAPIAIAGATLAPQSLWVIGVTALIVFALWGFFN
                     RTLTGKAILAVSHNRLAAQLMGISVRRVLLVSFALSAALGALAGILIAPITFTSWDVG
                     VMLGLKGFAAAILGGLGSGPGAVLGGLVLGLLESLGAGYVSSAYKDAIAFIVMLAVLM
                     LMPGGLLGGRAAERV"
     gene            6686..7603
                     /locus_tag="AC16O9_14375"
     CDS             6686..7603
                     /locus_tag="AC16O9_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517837.1"
                     /translation="MRGLLALAAVLALAPLAFANNYHYEIAILAGLNAIVCVGLNLLI
                     GYAGQVSLGHAGFFGIGAYGTAILAARYGWPPLAALAAATAGAAALAWVVGRPILRLR
                     GHYLAMATLGLGIIIAIVITTEDRFTGGPDGMSVPPLALAGWTLTGERVWYWLVGGLL
                     VAAVWLSLNLIDSPPGRALRALHGSEVAAEVAGIDSARHKLLAFVLSAGIAGLAGALT
                     AHYAGFITPGKVSFLHSIELVVMVVFGGMASTFGAVVGAAVLTALPQLLTVFKEYEMV
                     VFGAVMMLTMIFMPKGLVPTLAALARRRR"
     gene            7600..8361
                     /locus_tag="AC16O9_14380"
     CDS             7600..8361
                     /locus_tag="AC16O9_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517838.1"
                     /translation="MSLLRAEGLAKEFGGVRAVEGLDFEIRAGQVFSIIGPNGAGKTT
                     LLNLLTGIYAPSAGRLWLEGRALAARKPHEFAAAGIGRTFQNLQIFFNMSALENVMAG
                     RHLHERCSLAAALLRLPRRARAERDCRRLAAELLAFVGLEASVGAPADALPYGALKRL
                     EIARALAGEPRLLLLDEPAAGLNPSEIRDMDALIQRLAERGITVVLVEHNMKLVMGVS
                     DHVLVLDYGRKLAEGAPQIVRNDPRVIEAYLGAGD"
     gene            8363..9064
                     /locus_tag="AC16O9_14385"
     CDS             8363..9064
                     /locus_tag="AC16O9_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517839.1"
                     /translation="MLRVAALHSRYGRIPALKGIDLYVEAGELVSLVGANGAGKTTLL
                     RALSGVQPVAGGRVEFCGEDVTTATPEARVALGIVQVPEGRQVFGPLSVEDNLRLGAY
                     RRGRAQSAASLERVYALFPDLLERRAQPAGNLSGGQQQMLAMGRALMARPRLLLLDEP
                     SMGLAPRLVAQIFACVAQLRAADTALFLVDQNARAALAIADRGYVLETGSIVLAGTGA
                     QLLADAGVRSAYLGL"
     gene            9110..9523
                     /locus_tag="AC16O9_14390"
     CDS             9110..9523
                     /locus_tag="AC16O9_14390"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6517840.1"
                     /translation="MKPTLAPGITATRAFGVDRERTIDFMGEDARVYATPMLVRDIEV
                     TCRELLLAHLEPGEDSVGTRVEIDHLAATLFGMKVEITATLAELKGRAAVFDVVATDG
                     LDTICRGRHARFIANVAQVKERLKQKQAKAASKTA"
     gene            9520..12138
                     /locus_tag="AC16O9_14395"
     CDS             9520..12138
                     /locus_tag="AC16O9_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6517841.1"
                     /translation="MKRVATYCYQCVAGPDLLTVKVEGGVAREVEPNFAAAAVHPGGG
                     KVCVKAYGLVQKTYHPDRILQPMKRTNPKKGHDEDPGWTPIAWDEALELIAGKLRSVK
                     DRGLLDESGYPRVAASFGGGGTPQSYMGSFPAFLAAWGPVDMGFGSGQGVKCYHSEHL
                     YGELWHRGFTVSPDTPLTEYLISCGANVEASGGVVGVWRHANARARGMKRVQVEPHLS
                     VTGACSAEWVPIKPKTDAAFLYALVHVLLHEAPRERLDQAFLFGHTSSPYLVGAHGFY
                     LREPATGAPIVATSLEGRETHVALEIGADGEVLGEGKLEGETAFAKLVAHMQPYSPEW
                     AAAICDVPAARIRRIANEYLDAAHVGETIEIEGKTLPYRPVAVSLGKTVTNGWGGFEC
                     CWARTLLAALVGALEVPGGILGTTVRLNRPMSERHRSVRAGPDGLMAYPFNPTDARHW
                     SAQPNIRNAYRSMVPLAADGPWSQALGPTHFSWMFLDATPPGLPRVTLPEVWFLYRTN
                     PAISFWDTAALEERMARFPFVVAFAFTRDETNHYADVLLPDATDLESLQLIRIGGSKY
                     IEQFWDHEGFALRQPAIAPRGDARDFTDITTALAERTGLLEPYNAAINRGTLGVPLKD
                     FALDVLVAHKAESIWDAVCRAASFELSGGKESNGLAWWKDKGLATKPFPRTAWYLFPT
                     LAAKGLRFEMPYQERLLRIGTELGRRLHEHGMTWWDKQLAEYQALPAYKDFAGEWDRG
                     SRYPFWLLTSRSMQYAWGGNVGMQMIKEVADNIAGHGGVILNAAKARELGINEGDWLE
                     IATPAHSVRAKAVLRQGIRPDTLLLLGQFGHWATPVAKDFGMPSMNSLVPMSLELTDA
                     TGSGADIVRVSLRKTA"
     gene            12135..12734
                     /locus_tag="AC16O9_14400"
     CDS             12135..12734
                     /locus_tag="AC16O9_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6517842.1"
                     /translation="MTRWAMVADLRRCVGCQTCTAACKQTNDTPPGVQWRRVLDMEMG
                     EFPDVKRAFVPVGCMHCDDPPCMHVCPSTATRKRADGIVTIDYDLCIGCAYCAVACPY
                     QARYKSSDDALRPDVATKCTFCAERIDAGLAQGLKPGVDPEATPACVNSCIAQALHFG
                     DVEDPESNIAGLLAENQHFRMHEELGTGPRFYYLWDRKA"
     gene            12731..13492
                     /locus_tag="AC16O9_14405"
     CDS             12731..13492
                     /locus_tag="AC16O9_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517843.1"
                     /translation="MNWLQKHWDARAAANFMLGGAGAGLMIAAALGRLEGGAPVALAL
                     VLVAAGLGAVWLEIGKKLRALNVFLNPMTSWMTRESFVALLLFSFGAAALAKPLPFLP
                     LAALTALAFVFCQGRILRASKGIPAWRAPQVVLLIVATGLAEGAGLALLFSAAPELLA
                     WLVLALILRVYAWVCYAAAAPNAALEAPGKALVQIGTVGALAVALAALWLPAAAPLAG
                     ALALAPGLWLKYALVTRAALNQGFALRSAPVRGVR"
     gene            13599..14861
                     /locus_tag="AC16O9_14410"
     CDS             13599..14861
                     /locus_tag="AC16O9_14410"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR6517844.1"
                     /translation="MPRERLERLQLKRLKASLAAAYENVALHRQRMRARRLRPQDIRT
                     LEDLRRLPFTVKGDLRDQYPFGMFARPRASLVRLHASSGTTGKPTVVGYTPQDIDTWS
                     ALMARSMACAGVRPGDVVHNAYGYGLFTGGLGAHYGAERLGATVVPMSGGATERQVLL
                     MQDFGARVLCATPSYALNIAEVAEREGVDLRQSRLEIGLFGAEPWSEAMRREIEARLG
                     LRAIDLYGLSEIMGPGVAVECEAQDGLHGWEDHFLFEIIDPDSGQPVPEGEAGELVIT
                     TLTKQALPMIRYRTRDITRASTARCACGRTHLRLQRITGRNDDMLIIRGVNVYPSQIE
                     AVLVGLPGITPHYQLVVERRGSLDELTVEIEAASAELAGAELEERARHQIKSMIGVTA
                     SVVAKKPGEVPRSQGKAVRVRDLRPKTR"
     gene            14861..16096
                     /locus_tag="AC16O9_14415"
     CDS             14861..16096
                     /locus_tag="AC16O9_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517845.1"
                     /translation="MFANVARSRLLPMSLPFRYFGAAVAFQAAAWALLLANARALGTF
                     AAGLGPLFAALHLVTLGVLAMAAIGASLQLLPVATRQPVRAQWAAKLAWWLLVPGTAL
                     FAAGAAAYAVRAMAAGAVAAALALGIYGVLLFANLRGARGMRVVVAHGWAALACLAAL
                     AASGLALVARYEHGLELDHLALRNAHLVLAAYGFMGLLALGLSNFLLPMLAVAPPPAA
                     RRASAVLALAVAGIALALAGWLRAGALVGLAAAVGHVVLIEGSLRARLRAPLGPSFLL
                     VRASWGCLVASLALGALMAFDLAPAGAPVLFGLLLVPGWLLTFLLGVMQRIVPFLASV
                     HALSGGRPLASSLTPPRLLAAHAALHVGALALLLAGFHVAGAAAGLAAALAYAAFFVY
                     VLLKARHHGIRPRHQPAAA"
     gene            16059..16505
                     /locus_tag="AC16O9_14420"
     CDS             16059..16505
                     /locus_tag="AC16O9_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR6517846.1"
                     /translation="MAFDRAISRQLHEEHVATLTLWGRVEHSLAAGALDAALMREAAA
                     TLAGEIERHFEFEETQLFPRLAAAGEGDIAELLVEEHATIREAGARFMALARAEPPPP
                     ELRALALELAERLVSHVQKEEMALLPALDDLLDEQADRELQLVYAS"
     gene            16502..17431
                     /locus_tag="AC16O9_14425"
     CDS             16502..17431
                     /locus_tag="AC16O9_14425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6517847.1"
                     /translation="MTRKSTALEIRRLAPGDMDAVVALDAPLAGRTRRAYFERRLAAA
                     LRQPELHLQFAAASGKDLLGHALARVLEGEFGHAQPALRLEVISVAPKAQRRGIGRAL
                     HQALEEEARKRKLAELRTVAAWRDHDLLRFLDSLGYALAPNAVLECAVHGSPLLKRAE
                     QARDSVDVRTLSAKDLENVVRVDRHITGRERGAYVKHALDEALRESGVRISLAARIDD
                     VVCGYIMARADLGDFGRTEPVAVLDAIGVSPEYAERGVGRALLEQLFLNLEALRIERV
                     ETVVEARALDLLGFFHGAGFAPAQRLAFVKPTA"
     gene            17428..17817
                     /locus_tag="AC16O9_14430"
     CDS             17428..17817
                     /locus_tag="AC16O9_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR6517848.1"
                     /translation="MIEWDERYEVGVAVIDGQHREMLELVNRLLEGLRDGRDHDELVE
                     LLRALVRSAEHNVATEERLMQEHGLAIGHHAEEHQHMLESIRRFDLRLDSGGITASAR
                     WLSEWLLGHIDEDDRPFAEQLRARGVS"
     gene            17839..18585
                     /locus_tag="AC16O9_14435"
     CDS             17839..18585
                     /locus_tag="AC16O9_14435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6517849.1"
                     /translation="MKLDGMTAVVTGAASGIGRATAEAFGAAGAHVILGDVNAEGGEA
                     AAAAIRARGQGADFVRLDVTDPASVEAFRAAALARRNEVHIVANVAGWGRTEPFVQNA
                     PELWDKLVALNLMGPVRVSRAFLEPMIARGAGRIVNVASDAGRVGSLGETVYSAAKGG
                     AIAFTKSLAREVARYNITVNCVCPGPTDTPLMAAVPDKIKEAFTRVTPMRRLAKPGEI
                     ADAILFFASGRAAFVTGQVLSVSGGLTMAG"
     gene            18611..19429
                     /locus_tag="AC16O9_14440"
     CDS             18611..19429
                     /locus_tag="AC16O9_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HIR6517850.1"
                     /translation="MYKTKAAEWRPQHFGWQVKARVATITLNRPERKNPLTFESYAEL
                     RDTFHKLQYAEDVRAVVFTGEGGNFCSGGDVHEIIGPLVKMDMPDLMRFTRMTGNFVK
                     EMRNCPQPIIAAVDGVCAGAGAIISMASDLRYATPAAKTAFLFVRVGLAGCDMGACAL
                     LPRIIGQGRAAELLYTGRALGAEEGLAWGYFNEVVPAEKLLARAHEMAQSLADGPAFA
                     HAMTKKCLHQEWSLSIEQALETEAEAQAICMQTKDFERAYNAFVAKQKPRFEGD"
     gene            19451..21076
                     /locus_tag="AC16O9_14445"
     CDS             19451..21076
                     /locus_tag="AC16O9_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6517851.1"
                     /translation="MDRPSLDPHRLEAERRRGLWPQRVVTDALDRWVAEKPEACALVS
                     WREETGICEQLSYRDLAGRTARAAHALAKRGIGRGDVVAFQLPNCWQFVAAHLACVRL
                     GAVSNCLMPIFRHRELSYMLRHGEARALIAPSRYRGFDHGALARRLGRELPGLAQVLI
                     ADDTGADSFETALAAAEPSIAPGARLEPDDIMQLLFTSGTTGEPKGVLHTSNTLLSAV
                     HEYARRMRIGPDDVIFMPSPLGHQLGFCYGMLMALELGIPLVLTDVWNPKRALELMRE
                     HGATFSFGATPFLADLAGQPGVEAQRPPRLRLFASSGAPIPPAVVEAARTRLGVQVAA
                     CWGMTECASVTITPPEGTKVMESDGCALPNGEVRIVGADGREAARGVVGTLQVRGASL
                     FVGYLKRPELSRLEEGGWFDTGDLARMDDEGYIRICGRSKDVIIRGGENIPVIEIESA
                     LYRMSEVGDVAIVAMPDPRLQERACAFVTLHPNCTLTLEHVRRHLQAEGFSTHFWPER
                     LEILQEMPRTPTGKIQKFVLRERAKTLVAEGLS"
     gene            21085..21945
                     /locus_tag="AC16O9_14450"
     CDS             21085..21945
                     /locus_tag="AC16O9_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6517852.1"
                     /translation="MPASAVFSALAIHTLWGGNPVAVKLGLEAFPPLWSGFLRFALGC
                     VCVYAWARYVRISIRPTREEWPSLLAMGALFTVQIALLNYGFGGTTAANGAVLIASNP
                     IFGVLFAHFLVPGDRVGVARAVGVAVAFAGVALVITRGAGLAVASAGDWIVLASACLL
                     GLRLAVAGRLLKRVNDVRLTIWQMLLSLPLFLGGALAWEEIRWENIAVAPVAGILYQG
                     IVVAGLAFTVNFYLMRRYSPSVMISFNFVSPVAGVLLGVAILGERLAASLLGGMALVA
                     LGLALIARRR"
     gene            21960..22676
                     /locus_tag="AC16O9_14455"
     CDS             21960..22676
                     /locus_tag="AC16O9_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR6517853.1"
                     /translation="MSHHRYLLLLGVLFALVFGALAVAPSHRADWALENALVVAFVVA
                     VAVTYKRFTLSRISYTLIFVFLCLHEIGSHYTYAEVPYDAWFESLTGRTFNSLVGWER
                     NNFDRVIHFSYGLLLAYPVREVFWRVASARGFWSYFLPLDITMSTSMIYELVEWFAAE
                     VFGGELGQAYLGTQGDVWDAQKDMGLASLGALIAMLITYGINRVLQRDLAVEWAESLR
                     VKDPRPLGEDEIARMLRQRK"
     gene            22713..24713
                     /locus_tag="AC16O9_14460"
     CDS             22713..24713
                     /locus_tag="AC16O9_14460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6517854.1"
                     /translation="MCPMSCHPTLCGMLAEVRDGKLVGVKGDDANPDSRGFLCIRGQA
                     SREIFDNPNRLLHPLIRDRRADEFRRATWDEAFERIVAGIAQSPPANTAIWPGHGTFT
                     TNYGTRISAQLMARFANFHGSQFWNPTMICWGLGAFGLGLTGMLETNTKEDMGEHSRL
                     IVLWAANLASQPNTARHLLAAKRRGAHVVTIDVRHTEATAKSDEVLLIRPGTDTALAL
                     ALINVICAEKLHDAEFVARHAVGFGQLAAHVKEYTPEWAAAITGLAPERIAALARRYA
                     RTRPAMIVLGGSSMHKSDNGWQAGRAIACLPGLTGNVGIAGGGFGPRHGSGAHGRGLA
                     SIIEPGRRAPGTAMPNQMSAVIAALREGRIHNLLLMGTNMLSSFAAAGAVAEGLARTR
                     LVVSYDLFLNDTARRYADVVLPGTAWLEELGCKMTHTHLYLMEPALAPAGETRSVNQV
                     FKALGERLQLEGFYPWASDEALVDAILDHPCTGRATVASLRARGGIGALHISHVANPT
                     LEFDTPSRKIEFYSARAQALGLPPLPSFDAPAANAYPLPLTQGRTLTHFHAFYNNGRE
                     LPTLARRDTHPEVWLAPTDAKQRGIEDGAAIRVFNDRGSLAARAHVTERMPPGALWLR
                     DGWPGLNQLTDGAAVLPDEAAEIFAFSAGQSSFDARVEVAPA"
     gene            complement(24710..24958)
                     /locus_tag="AC16O9_14465"
     CDS             complement(24710..24958)
                     /locus_tag="AC16O9_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR6517855.1"
                     /translation="MALMINETCTACDACVPECPNDAITAGNPIYVIDPLKCTECVGA
                     KDEPQCRLVCPVSDCIVTNPDFVETPEQLQEKYQSLHG"
     gene            complement(24971..25816)
                     /gene="bcrD"
                     /locus_tag="AC16O9_14470"
     CDS             complement(24971..25816)
                     /gene="bcrD"
                     /locus_tag="AC16O9_14470"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HIR6517856.1"
                     /translation="MSIHTVGIDIGSGAVKAALFRSDGARTEWLAKRCERIRRRDPME
                     LAKQTYDEVLEERKLAAEDVAYTATTGEGENVKFATGHFYTMTTHARGGVYLMPGVSA
                     VVDIGALNGRAIYVDERGKVLSYKMTSQCASGSGQFLENIARYLGVAVEEIGRLSTTS
                     ENPEKVSSICAVLAETDVINMVSRGIATQDILKGIHLSMASRLVKLLKVTGIKSGKVL
                     LTGGLALDDGLLAALREELVTEKVAGIEVASHPDSIYAGAIGAALWGAFRHDKLKELQ
                     ARAAA"
     gene            complement(25813..27159)
                     /gene="bcrA"
                     /locus_tag="AC16O9_14475"
     CDS             complement(25813..27159)
                     /gene="bcrA"
                     /locus_tag="AC16O9_14475"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit A"
                     /protein_id="HIR6517857.1"
                     /translation="MRCFIGIDLGSTTTKAVVIDEQQRVLGRGITNSRSNYDTAATIA
                     KQEALLSARFHLFREALAASGALNGGLEDFVAQLERDFRLEQFLVQLADLEDVCERSV
                     ATARFGEAEKAVDEALVQVFARLRQEAPELFAPGAKRKSDFFRDIAGSRYHSLSEEVA
                     KEAGLRYDFLLNVFDRSIIEVENRVYDASISKNLRAALERTYRALPETAGKKAKISAP
                     IQGVLDLALEEAYVVGTGYGRARLPFSKEHIRSEILCHGLGAHVMYPSTATVLDIGGQ
                     DTKAIQVDPQGIVESFQMNDRCAAGCGRYLGYIADEMNMGLHELGPLAMKATKTVRIN
                     STCTVFAGAELRDRLSLGEKREDIMAGLHRAIILRALSILARSGGVRNEFTFTGGVAK
                     NEAAVKSLTELIEENYGKLKINIDPDSIYTGALGGATFAWRAVVEEGKAIEKRETA"
     gene            complement(27170..28531)
                     /gene="bcrB"
                     /locus_tag="AC16O9_14480"
     CDS             complement(27170..28531)
                     /gene="bcrB"
                     /locus_tag="AC16O9_14480"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HIR6517858.1"
                     /translation="MSTPIQIVPGSSTPKKPPQAKKEDAMLLQKELINRNYHELATAH
                     ETNRKVSATFVPGNLNELLMCFDFARSLPETNALQNGMRKKSGKMIMDAERDGHSEDV
                     CTYVKADLGMMLQGPVGPTGEPLPTPDVLLLSYTGCFTFMKWFELIRQKYDCETVMLH
                     VPYQGDGRISPNMRDYVVKQLKEKVIPTLEKVSGVKFDIDRLRQYMRESVKAEEDLVW
                     TLQSAKHKPSPIDGYFGAVYYIGPIFTAFRGMPEASEYYRVLRGEIELRMREGKGPVT
                     PDGEHPGEKYRLVVEGPPNWTSFREFWKMFYDEGAVVVASTYAKVGGVYDFGFRHDPE
                     HPLESLAEYCLGCYTNLNFPQRIDMICRYLDEYQADGLLINSIKSCNSFSAGQLLILR
                     EVERRTGKPAAFIESDLVDPRYFSAANIKNRLESYFQMVRQKRGAGGGARPGLPPVVP
                     AAH"
     gene            complement(28528..29694)
                     /gene="bcrC"
                     /locus_tag="AC16O9_14485"
     CDS             complement(28528..29694)
                     /gene="bcrC"
                     /locus_tag="AC16O9_14485"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HIR6517859.1"
                     /translation="MNAQEIVARCERLFEDLRFGAVKDWKAAVPGRKAIGYMPIYVPR
                     EIIHAAGMLPVGIFGGGDQLEVIQGDAYYQSYICRIPRSTLELGLTGRLDVLDGMLFP
                     SICDVIRNLSGMWQIMFKDKYVKYFDVPQNYDDAIGGEFYVHEMQQLRADLGKLAGRE
                     VTDDALRASIAVYNENRKAVRELYAYRAAAPWQAPTSELYLVLRAGCVLPPEEHTALV
                     RAYLAAARAEERPRRDNARVVLTGSFCEQPPLSLIKSIEMSGCYVVDDDFQLIQSWLL
                     DDVPVTGEPLEELSKAFLHRSAATSSKYDEKKEDKGKFLLKQIKERGAEGVVFAAASF
                     CDPALLERPMLQEVLAKHKVPYTAFKYAENTGQMAPIREQAGTFADSIKLWSAA"
     gene            complement(29691..30461)
                     /locus_tag="AC16O9_14490"
     CDS             complement(29691..30461)
                     /locus_tag="AC16O9_14490"
                     /EC_number="4.2.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF027439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexa-1,5-dienecarbonyl-CoA hydratase"
                     /protein_id="HIR6517860.1"
                     /translation="MSETPLKAWLERDGALLRLRLARPKANLIDAAMIAALSDAFAEY
                     RDNTGLLAALVDHEGPHFSFGASVEEHLPGACEQMLAGLHRLLGAMLEWPRPILMCVR
                     GQCLGGGLEFALAGNLLFAAPDARFGQPEMKLGVFAPAASVLLPLRIGQARAEDLLYS
                     GRSIDAPTALAWGLANEVAEDPAAAAAAYFGEHLAPKSAAALAHAVRAAREPFADLAR
                     ARLDDVEALYLDKLMATRDAREGLAAFMEKRAPKWEHR"
     gene            complement(30458..31567)
                     /gene="oah"
                     /locus_tag="AC16O9_14495"
     CDS             complement(30458..31567)
                     /gene="oah"
                     /locus_tag="AC16O9_14495"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HIR6517861.1"
                     /translation="MGKPDAKFLKDHDLPALAERGVRYEKRAAKTPDGKEVPGLHNAW
                     IWLDNPTQYNSYTTEMVKAVILAFRAASNARDVNCVVFTGAGDKAFCTGGNTKEYAEY
                     YAGHPQEYRQYMRLFNDMVSAILACDKPVICRVNGMRIGGGQEIGMACDFSVAQDLAR
                     FGQAGPKHGSAPIGGATDFLPVIAGAERAMAACVLCEPFSAHEAYHMGVLTDIVPALK
                     VDGKFVANPLVETQRVYDEFGRLVFGRPKTGEALAQGKALMKQGAVDLSKLDECVEAL
                     CAKILLTFPDCTTKTLEELRKPKLEAWNRNKEDARAWLALNMMTEARAGFTAFNSGPK
                     DDREVDFVLLRQKLAAGEGWVGALHDAIQPKAKRG"
     gene            31692..33386
                     /locus_tag="AC16O9_14500"
     CDS             31692..33386
                     /locus_tag="AC16O9_14500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6517862.1"
                     /translation="MLAHASVSLALAGSGGAGVMTAGNMLLEAAAQAGYYGLMTRTSG
                     PQIRGGEAAAMLRVSTFPGDGQDDAFHVLAAIDWQNVQRFAAELPLGPQSLIVGDPAH
                     GAPPEVFLRGGARYAPLPLKKLAGEVAGGWPNMVALGLLAALLGLPEAALEAAVRKSM
                     KKGLDAGLAAARLGRAQAAGLPPCTLAAPPAHAPRWLVTGNEAAGYGALRGGVRFVAA
                     YPITPATELLEYLAPALAQVGGTLVQAEDELASINMALGASYGGVPALTATSGPGLSL
                     MIESLGLGVAAEIPVVVVDVMRTGPSTGIATKSEQADLGTALYGLHGDAPHVVVAPNS
                     LADCLAATEWAVGLAESLQVPAIVLSDQYFGQARAVVDAPSASSGAVARLRAEAAGGY
                     QRYAVTDAGVSPMAIPGAPGLTYTADGLEHNERGTPSSQASDHLAQMDKRARKLARLD
                     PGEHWASVEGEGDTALITFGSCTGPAREALARNGNGVRLVSLRLLAPAQPERLKKALE
                     GVKRVLVLEQNHSGQFLRYLRAEYELPGEVRSLRRPGPLPFRPGEIRDALGQWSRT"
     gene            33383..34216
                     /locus_tag="AC16O9_14505"
     CDS             33383..34216
                     /locus_tag="AC16O9_14505"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6517863.1"
                     /translation="MNAPADATLCAPKDFKSDTKPVWCPGCGDYSVLAAITKALAALN
                     LRPENVVVVSGIGCSSRIPAYTACYGFHGVHGRALPVATGLKAARPDLTVLVASGDGD
                     GYSIGGNHFLHACRRNIDLTYIVMDNHVYGMTKGQPSPTTEPGWSSKLAPGGTGLKEF
                     HPLVIALASGANFIARAYSGDPNGCAALIAEAVRHPGFSFVEVLSPCVTFRPEQREWK
                     TLAHPAPVAPTDDPGRAARRLMTDDGLNIGVLYQGKRAPYARASGTEQTVEALACQFR
                     L"
     gene            34198..34707
                     /locus_tag="AC16O9_14510"
     CDS             34198..34707
                     /locus_tag="AC16O9_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR6517864.1"
                     /translation="MPVPTLEEFMARAYAMELEAHERYALFAEQLDTHNNRAVAEMFG
                     KLARIEALHAKRILEEMGWPSLPTLPPAYAWDTPEAPETAPPDAVHYLMQPYHALEIA
                     LRCELQAQKYFEDIAAASGVPPAVKKAALEMADEEREHVSLVRDWMKRVPVPPDGWDE
                     DPDPPRVTE"
     gene            34704..35105
                     /locus_tag="AC16O9_14515"
     CDS             34704..35105
                     /locus_tag="AC16O9_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6517865.1"
                     /translation="MRVLQPRGWANPVGYANGIEARGRVVFIAGQVGWDAAQKFHSQD
                     LAPQFEQALANVLAVLAEAGGRAEHICRMTAYCCDKPAYLAARPALGRIWRSLMGRHF
                     PAMSMIFVADLLDAPGKIELEATAVIPEETT"
     gene            35102..35803
                     /locus_tag="AC16O9_14520"
     CDS             35102..35803
                     /locus_tag="AC16O9_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6517866.1"
                     /translation="MKGLKGKTAIVTGGAAGIGAAIVERFRAEGTKVVVFDLKGSPSL
                     DITDYEAVKKAVAAAGPVDILVNNAGWDMFKPFLATDPAFWQKIISINLVGPMNLLHC
                     VLPGMAERGGGKVVNIASDAGRVGSSGEAPYSACKGGIIAMTKTLARELASKGVRLNT
                     VCPGLTETSMLEAFMQGAGNPDKLREAYRRAVPIGRLGKPEDIAGAVLFLASDDAEFI
                     TGQTISVSGGLTMHG"
     gene            complement(35790..>36148)
                     /locus_tag="AC16O9_14525"
     CDS             complement(35790..>36148)
                     /locus_tag="AC16O9_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6517867.1"
                     /translation="SHVGAVLVVRGEQLVGIFTERDALNRVMAEGRDPAATRLAEVMT
                     HDPKTVAADETLGYALLVMHEHGFRHLPVLEKRRPVGVISARHALDPDLEEFTAEAER
                     RKKIRRHAAGTRPSRA"
BASE COUNT         5051 a        12957 c        12554 g         5586 t
ORIGIN      
        1 tcgacctcgc cgaccttgcg cacgccggtc tgcaccttga ccatttcggc cttgccgtcg
       61 acgacgcgga acacgaacct gtcctggccg cgcggcacga tcgcctgctc gggcacccac
      121 accgcgttct cgcgcaccgc gagctgcgcc cacacgcgcg cgaacatccc cgggcgcagc
      181 ttgatctggg gattggcgac gcgcgcgcgc accagcaccg tgcgcgtcgc ctcgtccacc
      241 gccggctcga gggcgtacac cgagccggga aagctgcgct cgccgtaggc gtcgagctgc
      301 acgcgtaccg cctgtccggg cttcaggcgc ccgacgtaga tctcgggcac gcggaaatcg
      361 agcttcagct ggtcgatctt ctccaggcgc gcgatgtcgg tgccggcggc gacatacgcg
      421 ccctcgctca cctggcgcag gcccgcgacg cccgcgaagg gcgcgcggat cacggtcttg
      481 gcgaggcgcg cctcgtcctc gcgctgcttg gcggccgagc gcgccgcgtt ggcgcgcgcc
      541 tcgtcgtagg cctgctggct gatgaagttc ttcttgaaca ggtccgccgc gcgctcgagg
      601 cgctgccggt cgagctcggc ctgcgcgcgg ctcgaggcga gcacggcggc gtactcggcc
      661 tggtcgagct gcaccagcac cgcgccgcgc tcgacgcgct ggccttcctt gaagcggatc
      721 tcggcgatgc ggccggcgat ctcgggtcgg agggtcaccg cctcgtcggc acgcagcgtc
      781 cccaccgcag tcgcctcgtc gaccgcgggc gccgacttgg cgagcgcggc gcgcaccggc
      841 atagcgggcg cgggcttgcc ctgcgcgtgc gcccacgcgg gcgcgagcgc gatcaacagg
      901 gcgagggtca ggcgcatggc gattcagtaa gcgcctgatt ctacttgaag cgccgggggt
      961 tacggcgccg gatggccggg caggctggcg cgctttttcg ccaggcgctg ccgggtcttc
     1021 tccacgtcca ccacgaagcg cacgtgctcg ccgcgcccgc actgctcgac gttgtcgtgc
     1081 accgtaaagg cgcagcgcac gctgcggcct tccaccgcga ccacttcgta gcgcagcgtg
     1141 acctccatgc ccgcgggcgt ggcggccagg tggtcgatga ccacgcgcgt gccgaccgtg
     1201 tcctcgcccg cgtcgagatg ctcgagcagg aagtcgcgga tcgagtactc gacgtcgctc
     1261 accatgtgcg gcgtggcgta gaccgcgttc tccggcccca tgaacgcgat gcagcgcgcg
     1321 acgtccacgg tgagcgtgcg ctcgcccttg gcgccgggag cgagcgaggg cttcacgccg
     1381 ccttgtagcc ggcgcgctcg atggcctgct ttatcgcctc gacgctggtg tgcgcggggt
     1441 cgtaggtgac cttggcgcgc gccttggtga gcgacacgtc ggcctgggcg acacccggca
     1501 gggcggtgag aacgcgcgtc acgctgttca cgcagccggc gcaggtcatg ccttcaatgc
     1561 ccagtcttac ggtttccatc tggccgatcc gggacagcgt agcacggtga ggtccggccg
     1621 gcgtgttcag cgcgcgagca gcaccgggcg cttcgcgagg tgcagcacct tggtcgccac
     1681 cgacgcggcg agcaggttgg ccgccgcgcc catgccgctc gagccgatga cgatcaggtc
     1741 gcactttgtg cgctgcgcgt ggcgcacgat ggtctcggcc acggggccga catggacttg
     1801 cgcgtggtac ttgagacggg cgcgctgcag cctgcgctcg gcctgcttca ggttggccgc
     1861 cccctgcccc cggtaccact tcgcgatctc ggctttcttc atgcgcaggc cgacgcgcac
     1921 gagcttcggg atcggcgcgt gcacagtgag cagctcgacc gtgggcggct cgcggaactg
     1981 gttcgcctgc gcgacgagcc agtccaccgc ctcgagcgag gccttcgatc cgtccaccgc
     2041 gaccagaatc ttcatgcgcg ctcctgcacg gttgagcttg acctccatga tattccagcg
     2101 gcgggcgcgg ccttgacatg catcaagccg gctgccgcgc gcggcgcgta gcatgccgcg
     2161 cgcatgctga ttcccctgga ggcgccgtcg cagccggtcg atcccgcgcg ctttgcgctt
     2221 ttcgatctgg gcttccggcc gttctacctg ctcgccggcg catacgccgc gctcgcgata
     2281 ccgctgtggg ggctggagta cgcgggctgg ctgcccgggc cgggcatcct gtggcacgcg
     2341 cacgagatgg tcttcggcta cgccttcgcg gtgatcgcgg gtttcctgct cacggcggtg
     2401 cgtgcctgga ccggccggcc gacgcccagc ggcgccgcgc tcgccgcgct cgccggcctg
     2461 tggctcgccg cgcgcgcgct cgcctacgtg tcgttgccgc tcgcggcgtg ggccggggcg
     2521 ctgttcgcac tcgccctcgc cttcgccatc ggccggccgc tcgtcgccgc cggcaaccgg
     2581 cgcaacctgt tctttatcgc cctggtgctc gggctcggcg ctgcgggcat cgtggtgcag
     2641 cgccatccgc agctcggcct cgcgctcggc ctcgacgccg tgctcttcat catggcggta
     2701 atggcgggcc gcgtggtgcc cgccttcacc aacaacgcgg tggcgggcgc cggcgcgcgg
     2761 cgcctcccat ggctcgagtg gtcctcgctc ggcgcgctgc tcgcgctctt ggccgccgac
     2821 accctgcagc ggcccgagct cgccgcgggc ctcgcgctgc tcgccgcgcc gctgcacgcg
     2881 gcgcgccttg ccctgtgggc gccgggcaag gtactgcgcc ggccgatcct gtggatcctg
     2941 catctgtcct acgcctggat cgtcgtgcac ctgctgctgc gcggcttcac cggcttcggg
     3001 tttctgcctt atgcgctcgg cacgcacgcg ctcacggtag gcgccatcgg cgggctgacg
     3061 ctgggcatga tgacgcgcac ggcacgcggg catacggcgc ggccgctgca agcggggcgc
     3121 gcggaggtcg cggcctacct gctggtgcag ctcgccgcgg tcacgcgcgt gctggtgccg
     3181 ctcgccgtgc ccgccgccta cgtcggcgcg gccctcgccg cggcgctgtt ctggtttgcc
     3241 gcgttcgccg tcttcaccct tgcctacgtg ccgatcctca cgcgcccgcg gctcgacggc
     3301 cagccgggct gactcaggac cacggcgtgg cgtcgcgcgt cagcgcgacc cagacgatgt
     3361 acccggcgct ggcgagcgcg gcagcgaacg cggccgcgcg cacgcgcttc gtccggcccc
     3421 gcttcagcgc aatcgtgccg agggcgatgt aggcgacgag cacgagcacc ttggccgtga
     3481 tccagtcgtg caccaacggc acctggccga cgaaccaggc gagccacagc gcgctggcga
     3541 gcagcaccgt gtcgacgacg tgcggggcga cgcgcacgaa gcgcatctgc aaggacgcgg
     3601 accccatcag catgagagcg ccgcgggcga gaaagcccgc gatgcttatc gccgcgcagg
     3661 cgatgtgcag cgtcctgagg taggggtaga gctcagccgc gctcatggcg ggcgatgagc
     3721 ttcagcatgg tgtcgagcgt caccgagccg aaggtcgcgc gcgccgtctg gtcgatctcg
     3781 gcgagcaccc cgtcgagcgc ctgctcggcg ccgcggcgcg cccggcggcc gcgcggcacg
     3841 gcagagccga tgtcctcgaa cagcgcgacc acgtccatca gcgtcacgcg cttggcgttg
     3901 ccgacgaagc ggtagccgcc acgcgcgccg cgcaccgaat cgaccagccc cgcgcgcccc
     3961 agcatgcgca gcaccttggc gaggtggtgc ggcgagacgt gcagcgcggc ggcgatctcg
     4021 gtcaccgaca gctgccgccc gggccgcgca gcgaactcca gcgcgctgta gagcgcgagc
     4081 agcgtgttct tctgcagcct caaccgatgt ccttttcgag cggaatcacc ggcgcggcca
     4141 tcatgccctg gctgcggaag aacgacagca ccaggcggtt gtcgcgcgcg atcagcgtgc
     4201 gcaccgtggc aacgccctgg cgcccgaaag cggccgtgat cgccttgagc atcgccgtgg
     4261 ccactcccgc ctcgcgcgcc tgcgggcgca cgccgaggcc gaacacccag ccgcagggcg
     4321 gcgagccgaa ttcccagtcg cgcacctcgc cgacgatata gccgaggatg tccccgccgg
     4381 cctccgcgac caggaagcgc cggcggacgc ccccacggcc gtagcggcgc aggattcccg
     4441 cccagtactc gggcttggcg agcccggtgg cgcgctggtc gatcttgatc acctgcggca
     4501 ggtcggcgct ggctgcggcg cgcacgcgca gggcgcgacc ggccgcgccg cgtcgcctta
     4561 ttgcggctat ttcttgacgc atgtcaaacc gccggctccc cagccatata tacgctttac
     4621 ggtaccggat tgtcggattc aatgaaagga acgagatcat gaaactgcac gcgtggttcg
     4681 ctgccgcact gcttgccgcc gcctccgcta cgcaggccca ggcgccggtc cgcatcggtg
     4741 ccttcctgtc ggtcaccggt cccgccgcct tcctcggcga cccggagcaa aagacgctcg
     4801 agctctacgt agagcgcctg aacgccgcgg gcggcgtgct cggccgcaag ctcgagctcg
     4861 tcgcctacga ctcgggcggc gacgccgaga aggcacgcgc cttcgccaag cggttgatcg
     4921 agcaggacaa ggtcgacgtc atcgtcggcg gctcgaccac cggcgagacc atggccgtgg
     4981 tgccgctcgc cgaggccgcg ggcatgccgt tcatctcgct tgccggcgcc gtggtgatcg
     5041 tcgagccggt gaagaaatgg gtgttcaaga cgccgcacac cgaccgcatg gcctgcgaca
     5101 agatcttcgt cgacatgaag gcgcgcggcg tggccaaggc tgcgctcatc tcgggcagcg
     5161 gcggcttcga caagtcgatg cgcgcgcagt gccttggcgt ggcgaagaac cacggcatcg
     5221 acatcgtggc cgacgagacc tacggcgccg gcgacaccga catgacggcg cagctcacca
     5281 agatcaagaa tgcgaccggg gtgcaggcgg tgctgaacgc cggcttcggc caggggccgg
     5341 cgatcgtcac caagaactac aagcagctcg gcatcggcgt gccgctgtac cagtcccacg
     5401 gcgtcgcctc caagcagtac atccagctcg ccggcgacgc tgccgaaggc gtgcgcctgc
     5461 cggccgccgc gctgctggtg gccgaggcgc tcccggacag cgacccgcag aagaaggtgg
     5521 tgctcgagta caagcgggcc tatgaagagc gcttcaagca ggacgtgtcc accttcggcg
     5581 gtcacgccta cgacgggctg atgctcgccg tcgaggcgat gaagaaggcc ggcggcaccg
     5641 acaaggcgaa ggtgcgcgac gccctggagt cgattcgcgg ctacctgggc acggcgggcg
     5701 tggtcaacat gtcgccgcag gaccacatgg ggctcgatct cacggcgttc cgcgtgctgg
     5761 aggtgcgtaa gggcgactgg acgttggtga agtaggcggt gagcggcgag ctcgcccagt
     5821 tcctgctctc cggcctgacc gtcggggcga tctatgctct ggtggcgctc ggcttctcga
     5881 tcatctacaa cgcgagccac gtcatcaact tcgcgcaggg cgagttcgtg atgatcggcg
     5941 gcatggccac ggtgtcgctc ctcggcgccg gcctgccgct cgcgcccgcg gcgctcgccg
     6001 cggtcgcgct cgccgcgctc ctcggcctgc tgctggcgcg cttcgcggtg cagccggcgc
     6061 gcggcgcctc ggtggtgacg ctgatcatca tcaccatcgg cgcggcgatc ctgctgcgcg
     6121 ggctggcgag catcgtctgg gacaagcgca tccacgcgct gccggcgttc tcaggcgaag
     6181 cgccaatcgc catcgcgggc gccacgctcg cgccgcagag cctgtgggtg atcggcgtca
     6241 ccgcgctcat cgtgttcgcg ctgtgggggt tcttcaatcg gacgcttacc ggcaaggcga
     6301 tcctcgcggt gtcgcacaac cgcctggcgg cccagctgat gggcatcagc gtgcgccgcg
     6361 tgctcctcgt ctcgttcgcg ctgtcggcgg cgctcggcgc gctcgccggc atcctcatcg
     6421 cgccgatcac cttcacctcc tgggacgtcg gcgtgatgct cgggctgaag ggcttcgccg
     6481 ccgcgatcct cggcggcctg ggcagcggcc ccggcgcggt gctcgggggc ctcgtgctgg
     6541 ggctgctcga gtcgctcggc gccggctacg tttcctcggc ctacaaggac gccatcgcct
     6601 tcatcgtcat gctcgccgtg ctgatgctga tgcccggcgg gctgctcggc ggccgcgccg
     6661 ccgagcgcgt ctagcagccc atgccatgcg cggcttgctc gccctcgccg cggtgctcgc
     6721 cctcgcacca ctcgccttcg ccaacaacta ccactacgaa atcgcgatcc tcgccggcct
     6781 gaacgcgatc gtctgcgtcg ggctgaacct gctcatcggc tatgccggcc aggtgagcct
     6841 cgggcacgcc ggcttcttcg gcatcggcgc ctacggtacg gcgatcctcg ccgcgcgcta
     6901 cggctggccg ccgcttgccg ccctcgccgc ggcgaccgcc ggggcggcgg cgctcgcctg
     6961 ggtggtgggg cgcccgatcc tgcgcctgcg cggccactac ctcgcgatgg ccacgctcgg
     7021 gctgggcatc atcatcgcca tcgtcatcac cacggaggac cgcttcaccg ggggccccga
     7081 cggcatgagc gtgccgccgc tcgcgctcgc cggctggacg ttgaccggag agcgcgtgtg
     7141 gtactggctg gtcggggggc tgctggtcgc cgcggtgtgg ctgtcgctca acctgatcga
     7201 ctcgccgccc gggcgcgcgc tgcgcgcact gcacggctcg gaggtagccg cggaagtggc
     7261 cggcatcgac agcgcgcgcc acaagctgct cgcgttcgtg ctgtccgccg gcatcgcggg
     7321 gctcgccggc gcgctcaccg cgcactacgc cggcttcatc acgccgggca aggtgagctt
     7381 cctgcattcc atcgagctgg tcgtgatggt ggtgttcggc ggcatggcct ccaccttcgg
     7441 cgcggtggtc ggcgcggcgg tgctcaccgc gctgccgcaa ctgctcacgg tattcaagga
     7501 atacgagatg gtggtgttcg gcgccgtgat gatgctcacc atgatcttca tgccgaaggg
     7561 cctggtgccc acgctcgcgg cgctggcgag gcggcgccgg tgagcctgct gcgcgccgag
     7621 ggcctcgcca aggaattcgg cggcgtgcgc gcggtcgagg ggctggactt cgagatccgc
     7681 gccgggcagg tgttctcgat catcggcccg aacggcgccg gcaagaccac gctgctcaat
     7741 cttctcaccg gcatctacgc ccccagcgcc gggcggttgt ggctggaggg ccgcgcgctc
     7801 gcggcgcgca agccgcacga gttcgccgcc gcgggcatcg gccgcacgtt ccagaacctg
     7861 cagatcttct tcaacatgag cgcgctcgag aacgtgatgg ccgggcgcca cctgcacgag
     7921 cgctgctcgc tggcggcggc gctcctgcgc ttgccgcggc gcgcgcgcgc cgagcgcgac
     7981 tgccggcgcc tggccgcgga actgctcgcc ttcgtcggtc tggaggcgag cgtgggcgcg
     8041 cctgccgacg ccctgcccta cggtgccctc aagcggctgg agatcgcgcg cgccctcgcc
     8101 ggcgagccgc ggctgctgct gctcgacgag ccggcggcgg ggctcaaccc ttccgagatc
     8161 cgcgacatgg acgcgctcat ccagcgcctc gccgagcgcg gcatcaccgt ggtgctggtc
     8221 gagcacaata tgaaactggt gatgggggtc tccgaccacg tgctggtgct cgactacggc
     8281 cgcaagctcg ccgagggcgc gccgcagatc gtgcgcaacg atccgcgcgt catcgaggcc
     8341 tacctcggcg ccggcgactg acatgctgcg cgtcgccgcc ttgcacagcc gctacgggcg
     8401 cattccggcg ctcaagggca tcgacctgta cgttgaagcg ggcgagctcg tgtcgctggt
     8461 cggcgccaac ggcgcgggca agaccacgct gctgcgcgcg ctctccggcg tgcagccggt
     8521 ggcgggcggg cgcgtcgagt tctgcggcga ggacgtgacc accgctacgc ccgaggcgcg
     8581 cgtcgccttg ggcatcgtgc aggtacccga ggggcgccag gtgttcggtc cgctctcggt
     8641 cgaggacaac ctgcgcctgg gcgcctaccg ccgcggccgc gcgcagagcg cggcgtcgct
     8701 cgagcgcgtc tacgccctgt ttcccgacct cctcgagcgc cgcgcccagc ccgcgggcaa
     8761 cctttcgggc ggccagcagc agatgctcgc catgggccgc gcgctgatgg cgcgcccgcg
     8821 gctgctgctc ctcgacgagc cgagcatggg gctcgcgccg cgcctggtgg cccagatctt
     8881 tgcctgcgtg gcgcagctgc gtgccgccga caccgcgctc tttctcgtcg accagaacgc
     8941 gcgcgccgcg ctcgccatcg ccgaccgcgg ctatgtgctc gaaaccggca gcatcgtgct
     9001 cgccggcacc ggcgcgcagc tcctcgccga cgcgggcgtg aggagcgcct acctcggtct
     9061 ttaacctgga tcaaataccc cggacccgtt tgcgacaagg atagccccca tgaaacccac
     9121 cctcgccccc ggcatcaccg cgacgcgcgc attcggcgtc gaccgcgaac gcaccatcga
     9181 cttcatgggc gaggacgccc gcgtctacgc cacgccgatg ctggtgcgcg acatcgaggt
     9241 cacctgccgc gagctgctgc tcgcgcatct cgagccgggc gaggactccg tcggcacgcg
     9301 cgtcgagatc gaccacctgg cggcgacgct cttcggcatg aaggtcgaga tcaccgcgac
     9361 gctcgccgag ctcaagggcc gcgccgcggt gttcgacgtc gtcgccaccg acggcctcga
     9421 caccatctgc cgcggccggc acgcgcgctt catcgccaac gtcgcccagg tgaaggagcg
     9481 cctcaagcag aagcaggcca aggccgcctc gaagacggca tgaagcgcgt cgccacctac
     9541 tgctaccagt gcgtcgcggg gccggacctg ctcacggtca aggtcgaggg tggcgtcgcg
     9601 cgcgaagtcg agcccaactt cgccgccgcg gcggtgcacc cgggcggcgg caaggtgtgc
     9661 gtgaaggcct acggcctggt gcagaagacc taccacccgg accgcatcct gcagccgatg
     9721 aagcgcacca acccgaaaaa gggccacgac gaggatcccg gctggacgcc gatcgcgtgg
     9781 gacgaggcgc tcgagctgat tgccggcaag ctccggtccg tcaaggaccg ggggctgctc
     9841 gacgaatccg gctatccgcg cgtcgcggcg agcttcggcg gcggcggcac gccgcagtcc
     9901 tacatgggca gcttccccgc gttccttgcg gcgtgggggc cggtggacat gggctttggc
     9961 tccggccagg gggtgaagtg ctaccactcg gagcacctgt acggcgagct ctggcaccgc
    10021 ggcttcaccg tctcgcccga cacgccgctc accgagtacc tgatctcctg cggcgccaac
    10081 gtcgaagcct cgggcggcgt cgtcggcgtc tggcgccacg ccaacgcgcg cgcgcgcggc
    10141 atgaagcgcg tgcaggtcga gccgcacctt tcggtgaccg gcgcctgctc cgcggaatgg
    10201 gtgccgatca agcccaagac cgacgccgcc tttctctacg ccctggtcca cgtgctgctg
    10261 cacgaggcgc cgcgcgagcg gctggaccag gcctttctct tcgggcacac ctcgtcgccc
    10321 tacctggtcg gcgcgcacgg cttctatctg cgcgagccgg cgaccggcgc gccgatcgtc
    10381 gcgacttcgc tcgaaggaag agaaacgcac gtcgctctcg agatcggcgc cgacggcgag
    10441 gtgctcggcg aaggcaaact cgagggcgaa accgcgttcg ccaagctcgt cgcgcacatg
    10501 cagccctact cgcccgaatg ggccgcggcg atctgcgacg tgcccgcggc gcgcatccgc
    10561 cgcatcgcca acgagtacct ggacgccgcg cacgtcggcg agacgatcga gatcgagggt
    10621 aagaccctgc cttaccgccc ggtcgccgtg agcctcggca agacggtgac caacggctgg
    10681 ggcggcttcg agtgctgctg ggcgcgcacg ctccttgccg cgctcgtggg cgcgctcgaa
    10741 gtccccggcg gcattctcgg cacaacggtg cggctcaacc gcccgatgtc ggagcgccac
    10801 cggagcgtgc gcgccggccc cgacggcctg atggcctacc cgttcaatcc gaccgatgcg
    10861 cggcactgga gcgcgcagcc caacatccgc aacgcctacc gctcgatggt gccgctcgcc
    10921 gccgacggcc cgtggagcca ggcgctcggg ccgacgcact tttcctggat gttcctcgac
    10981 gcgacgccgc cgggcctgcc gcgcgtcacg ctgcccgagg tgtggttcct gtaccgcacc
    11041 aaccccgcga tctcgttctg ggacaccgcc gccctcgaag agcgcatggc gcgctttccg
    11101 ttcgtggtcg cctttgcgtt cacgcgcgac gagaccaatc actatgccga cgtgctgctg
    11161 ccggacgcca ccgacctgga gagcctgcag ctgatccgca tcggcggctc gaagtacatc
    11221 gagcagttct gggaccacga gggcttcgcg ctgcgccagc ccgccatcgc gccgcgcggc
    11281 gacgcgcgcg acttcaccga catcaccacc gcgctcgccg agcgcacggg cctgctcgag
    11341 ccctacaacg ccgcgatcaa ccgcggcacg ctcggcgtgc ccctgaagga tttcgcgctc
    11401 gacgtgctcg tcgcgcacaa ggccgagagc atctgggacg cggtgtgcag ggcggcgagc
    11461 ttcgagctga gcggcggcaa ggaatcgaac ggcctcgcct ggtggaagga caaaggcctt
    11521 gcgaccaagc ccttcccgcg caccgcgtgg tacctgtttc cgacgctggc ggcgaagggc
    11581 ctgcggttcg agatgcccta ccaggagcgg ctgctcagga tcggcaccga gctcggccgg
    11641 cgcctgcacg agcacggcat gacctggtgg gacaagcagc tcgccgagta ccaggcgctg
    11701 ccggcgtaca aggatttcgc cggcgagtgg gaccgcgggt cgcgctatcc gttctggctg
    11761 ctcacctcgc gctcgatgca gtacgcgtgg ggcggcaacg tcggcatgca gatgatcaaa
    11821 gaggtcgccg acaacatcgc cggccacggg ggcgtgattt tgaacgcggc aaaggcgcgc
    11881 gagctcggca tcaatgaagg cgactggctc gagatcgcca cgcccgcgca ttcggtgcgc
    11941 gcgaaagccg tgctgcgaca gggcatccgc ccggatacgc tgctgctgct cggccagttc
    12001 gggcactggg cgacgccggt ggcgaaggac ttcggcatgc cctcgatgaa cagcctggtg
    12061 ccgatgtcgc tcgagctcac ggacgccacc ggctcgggcg cggacatcgt ccgcgtgtcc
    12121 ctgaggaaga ccgcatgacg cgctgggcga tggtcgcgga cctgcggcgc tgcgtcgggt
    12181 gccagacctg caccgcggcg tgcaagcaga cgaacgacac gccgccgggc gtgcagtggc
    12241 ggcgcgtgct cgacatggag atgggagagt ttcccgacgt gaagcgcgcc ttcgtgccgg
    12301 tgggctgcat gcattgcgac gacccgccgt gcatgcacgt gtgcccgagc acggcgacgc
    12361 gaaagcgcgc cgacggcatc gtcaccatcg attacgacct gtgcatcggc tgcgcctact
    12421 gcgcggtcgc ctgtccgtac caggcgcgct acaagtcgag cgacgatgcg ctgcggccgg
    12481 acgtggcgac gaaatgcacg ttctgcgcgg agcgcatcga cgcagggctt gcccaggggc
    12541 tcaagcccgg ggtcgacccg gaagcaacgc cggcgtgcgt gaattcctgc atcgcgcagg
    12601 cgctgcactt cggcgatgtg gaagacccgg aaagcaacat cgccgggctg ctcgccgaga
    12661 accagcactt ccgcatgcac gaggagctcg gcaccgggcc gcgcttttac tacctgtggg
    12721 accgcaaggc atgaactggc tgcagaagca ctgggacgcg cgcgcggcgg cgaacttcat
    12781 gctcggcggc gccggcgccg ggctgatgat cgcggcggcg ctcggccgcc tcgagggcgg
    12841 cgcgccggtc gcgctcgcgc tcgtactcgt ggcggcgggc ctgggcgccg tgtggctcga
    12901 gatcggcaag aagctgcgcg cgctcaacgt gttcctcaat cccatgacct cgtggatgac
    12961 gcgcgagtcg ttcgtggcgc tgctgctgtt ctccttcggc gccgcggcgc tcgccaagcc
    13021 gctgcctttc ctgccgctcg ccgccctcac cgcgctcgcc ttcgtctttt gccagggccg
    13081 catcctgcgc gcctccaagg gcatcccggc gtggcgcgcg ccgcaggtgg tgctgctcat
    13141 cgtcgcgacc gggctcgccg agggcgcggg cctggcgctc ctgttcagcg cggcgccgga
    13201 gcttctcgcc tggctcgtgc tggcgctgat cctgcgcgtc tacgcgtggg tgtgctacgc
    13261 cgcggcggcg ccaaacgccg cgctcgaggc gccgggcaag gcgctcgtgc aaatcggcac
    13321 cgtgggggcg ctcgcggtcg cgctcgcggc gctgtggctg ccggccgcgg cgccgcttgc
    13381 cggcgcgctc gcgcttgccc cggggctgtg gctgaagtac gcgctggtga cgcgcgccgc
    13441 cctcaaccag ggattcgccc tgcgcagcgc gccggtgcgg ggcgtacgct gaggggccgc
    13501 ccatgaccgc gatgctgcaa ccccggtacg ccgccaaggt gcacacgcgg gcaagcgctg
    13561 cacgccgccg ctattacttc gagcgcgaag cggaaacaat gccgcgcgag cggctcgagc
    13621 ggctgcagtt gaagcgcctg aaggcgagcc tcgccgcggc gtatgaaaac gtcgcgctgc
    13681 accgccagcg gatgcgcgcc cggcgcctgc ggccgcagga catccgcacg ctggaggacc
    13741 tgcgccggct gccgttcacg gtaaaaggcg acctgcgcga ccagtacccc ttcggcatgt
    13801 tcgcgcgccc ccgcgcgtcg ctcgtgcgcc tgcatgcctc ctccggcacc accggcaagc
    13861 cgaccgtggt cggctacacg ccgcaggaca tcgacacctg gtccgcgctg atggcgcgct
    13921 ccatggcctg cgcgggggta cggcccggcg acgtcgtgca caacgcctac ggctacggcc
    13981 tgttcacggg cggcctgggc gcgcactacg gcgccgagcg actcggcgcc accgtggtgc
    14041 cgatgtccgg cggggccacc gagcggcagg tgttgttgat gcaggatttt ggcgcgcgcg
    14101 tgctgtgcgc cacgccctcc tacgcgctca acatcgccga ggtcgccgag cgcgaaggcg
    14161 tggatctgcg ccagagccgc ctcgagatcg gattgttcgg cgccgagccc tggagcgaag
    14221 cgatgcgccg cgagatcgag gcgcgcctgg gcctcagggc gatcgacctc tacggcctgt
    14281 cggaaatcat ggggccgggg gtcgcggtgg agtgcgaggc gcaggacggc ctgcacggct
    14341 gggaggacca cttcctgttc gagatcatcg acccggacag cggccagccc gtgcccgagg
    14401 gcgaggcggg cgagctggtg atcacgacgc tcaccaagca ggcgctgccc atgatccgct
    14461 accgcacgcg cgacatcacg cgcgcgagca ccgcgcgctg cgcctgcggg cgcacgcatc
    14521 tcaggctgca gcgcatcacc gggcgcaatg acgacatgct catcatccgc ggcgtcaacg
    14581 tctacccgtc gcagatcgag gcggtgctgg tcggcctgcc cggcatcacc ccgcactacc
    14641 agctggtcgt cgagcgccgc ggcagcctcg acgagctcac cgtggaaatc gaagcggcct
    14701 cggccgagct cgccggcgcc gagctcgagg agcgcgcgcg gcaccagatc aagtcgatga
    14761 tcggcgtcac tgccagcgtg gtggcgaaga agcccggcga agtgccgcgc tcgcagggca
    14821 aggcggtgcg cgtgcgcgac ctcaggccga agacgcgctg atgttcgcca acgtcgcgcg
    14881 cagccgcctg ctgccgatgt cgctgccgtt tcgctacttc ggcgcggcgg tcgcgttcca
    14941 ggccgcggcg tgggcgctgc tccttgccaa cgcgcgcgcg ctcggcacgt tcgccgccgg
    15001 cctggggccg ctgttcgccg cgctgcacct cgtcaccctc ggcgtgctgg cgatggcggc
    15061 gatcggcgcg tcgctgcagc tcctgccggt cgccacgcgc cagccggtgc gcgcgcagtg
    15121 ggcggcgaag ctcgcatggt ggctgctcgt gccgggcacg gcgctgtttg ccgccggcgc
    15181 cgcggcctac gcggtgcgcg cgatggccgc gggcgcggtg gcggccgccc tcgcgctcgg
    15241 catctacggc gtgctgctct tcgccaacct gcgcggcgcg cgcggcatgc gcgtggtggt
    15301 ggcgcacggg tgggcagcgc tcgcctgcct cgccgcgctc gccgccagcg gccttgcgct
    15361 cgtggcgcgc tacgagcacg ggctcgagct cgatcacctg gcattgcgca acgcgcacct
    15421 ggtgctcgcc gcctacggct tcatgggcct gctcgcgctc gggctgtcga acttcctgct
    15481 gccgatgctc gccgtggcgc cgccgcctgc cgcgcgccgc gcgagcgccg tgctcgcgct
    15541 cgccgtcgcg ggtatcgcgc tcgcgctcgc cggctggctg cgggccggtg cgctggtggg
    15601 cctcgcggcc gccgtcggcc acgtcgtgct catcgagggc agcctgcgcg cgcgcctgcg
    15661 cgcgccgctc gggccgtcgt tcctgctggt gcgcgcctcc tggggctgtc tcgtcgcgag
    15721 cctcgcgctt ggtgcgctga tggcgttcga tctcgcgccc gcgggcgccc cggttctgtt
    15781 tggcctgctg ctggtgccgg ggtggctgtt gacgttcctg ctcggcgtga tgcagcgcat
    15841 cgtgcccttt ctcgcctcgg tgcacgcgct ctcgggcggg cggccgctcg cctcgtcgct
    15901 cacgccaccg cggctgctcg ccgcgcacgc ggcgttgcac gtcggcgcgc tggcgctgct
    15961 gctcgccggg ttccacgtcg cgggcgccgc cgccgggctg gccgccgcac tggcgtacgc
    16021 cgcgttcttc gtctacgttc tcttgaaggc ccgacaccat ggcattcgac cgcgccatca
    16081 gccggcagct gcatgaagag cacgtcgcga ccctcaccct ctgggggcgc gtcgagcaca
    16141 gcctcgcggc cggcgcgctc gacgcggcgc tgatgcgcga ggccgccgcc acgctcgccg
    16201 gcgagatcga gcgccacttc gagttcgagg aaacgcagct cttcccgcgg ctcgccgcgg
    16261 caggcgaagg cgacatcgcc gagctccttg tcgaggagca cgccacgatt cgcgaggcgg
    16321 gcgcgcgttt catggcgctc gcgcgcgccg agcccccgcc gcccgagtta cgcgcgctcg
    16381 ccttggagct cgccgagcgt ctcgtcagcc acgtgcagaa ggaggagatg gcgctgctgc
    16441 ccgcgctgga cgacctgctc gatgagcagg ccgaccgcga gctgcagctc gtctacgcgt
    16501 catgacccgg aaatcgaccg cgctggagat tcgccgcctc gcgcccggcg acatggacgc
    16561 cgtggtggcg ctcgacgcgc cgctcgccgg ccgcacccgc cgcgcctact tcgagcgccg
    16621 cctcgccgcg gcgctgcgcc agcccgagct gcacctgcag ttcgccgcgg cctcgggcaa
    16681 ggacttgctc ggccacgcgc tcgcgcgcgt gctcgagggc gagttcggcc acgcgcagcc
    16741 ggcgctgcgt ctcgaggtga tcagcgtcgc gcccaaggcg cagcgccgcg gcatcggccg
    16801 cgcgctgcat caggcgctcg aggaggaggc gcgcaagcgc aagctcgccg agctgcgcac
    16861 cgtcgccgcc tggcgcgatc acgacctgct gcggtttctc gacagcctgg gctacgcgct
    16921 ggcgccgaac gcggtgctcg aatgcgcggt gcacggcagc ccgctgctca agcgcgccga
    16981 gcaggcgcgc gactcggtcg acgtgcgcac gctctcggcc aaggacctcg agaacgtggt
    17041 gcgggtcgat cgccacatca cggggcgcga gcgcggcgcg tacgtgaagc acgcgctcga
    17101 cgaggcgctg cgcgagtcgg gcgtgcgcat ctcgctcgcc gcgcgcatcg acgacgtggt
    17161 ctgcggctac atcatggcgc gcgcggacct gggcgatttc ggccgcaccg agccggtggc
    17221 ggtgctcgat gcgatcggcg tgtcgcccga gtacgccgag cgcggcgtgg gccgggcgct
    17281 gctcgagcag ctgttcctca acctcgaggc gctgcgcatc gagcgcgtcg agaccgtggt
    17341 cgaggcgcgc gcgctggatc tgctcgggtt cttccacggc gcgggcttcg cgccggcgca
    17401 gcgcctcgcg ttcgtcaagc cgaccgcatg atcgagtggg acgagcgcta cgaggtcggc
    17461 gtcgcggtga tcgacggcca gcaccgcgag atgctcgagc tcgtcaaccg cctgctcgag
    17521 ggcctgcgcg acggccgcga ccacgacgag ctggtggagc tgctgcgcgc gctggtgcgc
    17581 tcggccgagc acaacgtcgc caccgaggag cggctgatgc aggagcacgg gctcgccatc
    17641 ggccaccacg ccgaggagca ccagcacatg ctcgaatcga tccgccggtt cgacctgcgc
    17701 ctggactccg gcggcatcac cgcaagcgcc cgctggctga gcgagtggct gctcggccac
    17761 atcgacgagg acgaccggcc gttcgccgag cagcttcgcg cgcgcggcgt gagctgaaga
    17821 tacgcggagg atagacgcat gaaactcgat ggcatgaccg cagtggtgac cggcgccgcc
    17881 tccggcatcg gccgcgcgac cgcggaggcg ttcggcgccg cgggcgcgca cgtgatcctc
    17941 ggcgacgtga acgcggaagg cggcgaggcg gcggcggcgg cgatccgcgc gcgcggccag
    18001 ggcgcggact tcgtgcggct ggacgtcacc gacccggcgt cggtcgaggc gttccgcgcc
    18061 gcagcgctcg cgcgccggaa cgaggtgcac atcgtcgcca acgtcgccgg ctggggccgg
    18121 accgagccgt tcgtgcagaa cgcgccggag ctctgggaca agctggtggc gctcaacctg
    18181 atgggcccgg tgcgcgtgag ccgcgccttc ctcgagccga tgatcgcgcg cggggcgggc
    18241 cgcatcgtca acgtggcgag cgacgcgggc cgcgtcggca gcctcggcga aaccgtgtac
    18301 tcggccgcca agggcggcgc catcgccttc accaagtcgc tggcgcgcga agtggcgcgc
    18361 tacaacatca ccgtcaactg cgtctgcccc ggccccaccg acacgccgct gatggccgcg
    18421 gtgcccgaca agatcaagga ggccttcacg cgcgtcacgc cgatgcgccg cctggccaag
    18481 ccgggcgaga tcgccgacgc gatcctgttc ttcgccagcg gccgcgcggc cttcgtcacc
    18541 gggcaggtgc tgtcggtgag cggcggcctg accatggccg gctgaacttc tcaaccagga
    18601 gcacgacatc atgtacaaga ccaaggcggc ggaatggcgg ccgcagcatt tcggctggca
    18661 ggtcaaggca cgcgtcgcca ccatcacgct caaccggccc gagcgcaaga acccgctcac
    18721 tttcgagagc tatgccgagc tgcgcgacac cttccacaag ctgcagtacg ccgaggacgt
    18781 gcgcgcggtg gtgttcaccg gcgaaggcgg caacttctgc tcgggcggcg acgtgcacga
    18841 gatcatcggc ccgctggtga agatggacat gccggacctg atgcggttca cgcgcatgac
    18901 gggcaacttc gtcaaggaga tgcgcaactg cccgcagccg atcatcgcgg cggtggacgg
    18961 cgtgtgcgcg ggcgcgggcg ccatcatctc gatggcgagc gacctgcgct acgccacgcc
    19021 cgcggccaag accgcgttcc tgttcgtgcg cgtgggcctg gccggctgcg acatgggcgc
    19081 ctgcgccctc ctgccgcgca tcatcggcca ggggcgcgcc gcggagctgc tctacaccgg
    19141 gcgcgcgctc ggcgccgagg aaggcctcgc ctggggctac ttcaacgagg tggtgcccgc
    19201 ggagaagctc ctggcacgcg cgcacgaaat ggcgcagtcg ctcgccgacg gccccgcctt
    19261 cgcccacgcc atgaccaaga agtgcctgca ccaggaatgg agcctgtcca tcgagcaggc
    19321 gctcgagacc gaggccgagg cgcaggcgat ctgcatgcag acgaaggatt tcgagcgcgc
    19381 ctacaacgcc ttcgtcgcca agcaaaagcc caggttcgag ggcgactgag ccggcggggc
    19441 ggcgcgcgcg atggaccgcc cctcgctcga cccgcatcgg ctcgaggcgg agcgccggcg
    19501 cggcctgtgg ccgcagcgcg tcgtcaccga cgcgctcgac cgctgggtcg ccgagaagcc
    19561 cgaggcctgc gcgctcgtgt cgtggcgcga ggaaacgggc atctgcgagc agctcagcta
    19621 ccgcgacctg gccggccgta cggcgcgcgc ggcgcacgcg ctcgccaagc gcggcatcgg
    19681 gcgcggcgac gtggtcgcgt tccagctgcc gaactgctgg cagttcgtgg ccgcgcatct
    19741 cgcctgcgtg cgcctgggcg cggtgagcaa ttgcctgatg ccgatcttcc gccaccgcga
    19801 gctctcttac atgctgcgcc acggcgaggc gcgcgcgctg atcgctccgt cgcggtatcg
    19861 cggcttcgac cacggggcgc tggcgcgccg gctcggtcgc gagctgcccg ggctggcgca
    19921 ggtgctgatc gccgacgata ccggcgcgga ttcgttcgaa accgcgctcg cggcggcgga
    19981 gccgtcgatc gcgcccggcg cgcggctcga gcccgacgac atcatgcagc tgctgttcac
    20041 ttccggcacc accggcgagc ccaagggcgt gctgcatacc tcgaatacgc ttctcagcgc
    20101 ggtgcacgag tatgcgcggc gcatgcgcat tggtcccgac gacgtcatct tcatgccttc
    20161 gccgcttggc catcagctcg gcttttgcta cggcatgctg atggcgctcg agctcggcat
    20221 cccgctcgtg ctcaccgacg tctggaaccc caagcgcgcc ctcgagctga tgcgcgagca
    20281 cggcgccacg ttctcgttcg gcgcgacgcc gtttctcgcc gacctcgcgg gacagcccgg
    20341 cgtcgaggcg cagcggccgc cgcggctgcg gctcttcgcc agctcgggcg cgccgatccc
    20401 gccggcagtg gtggaagcgg cgcgcacgcg gctcggcgtc caggtggccg cctgctgggg
    20461 catgaccgag tgcgccagcg tcaccatcac cccgcccgag ggcaccaagg tgatggagtc
    20521 ggacggctgc gcgctgccga acggcgaggt gcgcatcgtc ggcgcggacg gccgcgaggc
    20581 cgcgcgcggc gtggtcggga cgctgcaggt gcgcggcgcg tcgctgttcg tgggttacct
    20641 gaaacgcccc gagctctcgc gcctcgagga aggcggctgg ttcgacacgg gcgacctcgc
    20701 gcgcatggac gacgagggct acatccgcat ctgcgggcgc agcaaggatg tcatcatccg
    20761 cggcggtgag aacatcccgg tgatcgagat cgaaagcgcg ctctatcgca tgagcgaagt
    20821 gggcgacgtc gccatcgtgg cgatgcccga tccgcggctg caggagcgtg cctgcgcttt
    20881 cgtgacgctg cacccgaatt gcacgctgac gctcgagcac gtgcgccggc atctccaggc
    20941 cgagggcttc tccacgcact tctggcccga gcggctcgag atcctgcagg agatgccgcg
    21001 cacgccgacg ggcaagatcc agaagttcgt gctgcgcgag cgggcgaaga cgctcgtcgc
    21061 cgaggggctt tcctgaactg gtcgatcccg gcgagcgccg tcttctcggc gctggcgatc
    21121 catacgctgt ggggcggcaa tcccgttgcg gtgaagctcg ggctggaggc gttcccgccc
    21181 ttgtggagcg ggttcctgcg ctttgcgctg ggctgcgtgt gcgtctacgc ctgggcgcgg
    21241 tacgtacgga tttcgatccg gcccacgcgc gaggagtggc cgtcgctgct cgccatgggt
    21301 gcgctgttca ccgtccagat cgcgctgctg aactacggct tcggcggcac caccgcggcg
    21361 aacggcgcgg tgctgatcgc cagcaatccg atcttcggcg tgctgttcgc gcactttctc
    21421 gtgcccggcg accgcgtcgg cgtggcgcgg gcggtcggcg tcgccgtcgc attcgccggc
    21481 gtggcgctgg tgatcacccg cggcgccggg ctggcggtcg ccagcgccgg cgactggatc
    21541 gtgctggcga gcgcgtgcct gctcgggctg cggctcgcgg tcgccgggcg gctgctgaag
    21601 cgggtgaacg acgtgcggct gacgatctgg cagatgctgc tttcgttgcc gttgttcctg
    21661 ggcggggcgc tggcctggga ggagattcgc tgggagaaca ttgcggtcgc gccggtggcg
    21721 gggatcctgt atcaggggat cgtcgtggcc gggctggctt tcaccgtgaa cttctatctg
    21781 atgcgccgct actcgccgag cgtgatgatc agcttcaatt tcgtctcgcc ggtcgcgggg
    21841 gtgctgctgg gcgtggcgat tctcggcgag cgcctggcgg cgagcctgct cggcgggatg
    21901 gcgctggtcg cgctgggatt ggcgctgatc gcccggcgcc gttaagctgc gcccaggccg
    21961 tgagccacca tcgctacctg ctgctgcttg gcgtgctgtt cgccctggtg ttcggggcgc
    22021 tcgccgtcgc gccctcgcat cgcgccgact gggcgctgga gaacgcgctg gtggtcgcct
    22081 tcgtggtcgc ggtcgccgtc acctacaagc gcttcacgct gtcgcggatc tcgtacacgc
    22141 tcatcttcgt cttcctctgc ctgcatgaaa tcggctcgca ctacacgtac gccgaggtgc
    22201 cgtacgacgc ctggttcgag tccctcaccg gcaggacgtt caacagcctc gtcggctggg
    22261 agcgcaacaa cttcgaccgc gtgatccact tctcgtacgg gctgctgctt gcctacccgg
    22321 tgcgcgaggt gttctggcgc gtggcctcgg cgcgcggctt ctggagctac ttcctgccgc
    22381 tcgacatcac catgtccact tcgatgatct acgagctggt cgagtggttc gccgccgagg
    22441 tgtttggcgg cgagctgggc caggcctacc tcggcaccca gggcgacgtc tgggacgcgc
    22501 aaaaggacat ggggctggcg agcctgggcg cgctgatcgc gatgctcatc acctacggca
    22561 tcaaccgcgt gctgcagcgc gacctggcgg tggagtgggc cgaaagcctg cgggtgaagg
    22621 atccgcggcc cctcggcgag gacgaaatcg cgcgcatgct gaggcagcgg aagtagcgcg
    22681 tgacacggtc agccctgaac accgtccgca cgatgtgccc catgagctgc catccgacgc
    22741 tctgcggcat gctggcggaa gtcagggacg gaaaactcgt cggcgtcaaa ggcgacgacg
    22801 ccaaccccga cagccggggg ttcctgtgca ttcgcggcca ggcctcgcgc gagatcttcg
    22861 acaatcccaa ccgcctgctt cacccgctga tccgcgacag gcgcgcggac gaattcaggc
    22921 gcgcgacctg ggacgaagcc ttcgagcgca tcgtcgcagg catcgctcag tcgcccccgg
    22981 caaacacggc gatctggccc ggccacggca ccttcaccac caactacggc acgcgcatca
    23041 gcgcgcaact gatggcgcgc ttcgccaact tccacggcag ccagttctgg aacccgacga
    23101 tgatctgctg gggcctgggc gccttcggcc tgggcctcac cggcatgctc gagaccaata
    23161 ccaaggagga catgggcgag cattcgcggc tcatcgtcct ctgggccgcg aatctcgcca
    23221 gccagcccaa cacggcgcgg catctgctcg ccgccaagcg ccgcggcgcg cacgtcgtga
    23281 ccatcgacgt gcgccacacc gaggccacgg ccaagtcgga tgaggtcctc ctcatccgcc
    23341 cgggcacgga cacggcgctc gcacttgcgc tgatcaacgt catctgcgct gaaaagctcc
    23401 acgacgccga gttcgtcgca aggcacgccg tcggtttcgg gcagctcgcc gcgcacgtaa
    23461 aggaatacac gcccgaatgg gccgcggcaa tcaccggcct cgccccagag cgcatcgcgg
    23521 cgctggcgcg ccgctacgcc cgcacgcgcc cggcgatgat cgttcttggc ggcagctcga
    23581 tgcacaagag cgacaacggc tggcaggccg ggcgcgcgat cgcctgcctg cccgggctga
    23641 ccggcaatgt cggcatcgcg ggcggcggct tcggtccgcg gcacggcagc ggcgcgcacg
    23701 gccggggact cgccagcatc atcgagcccg ggcggcgcgc cccgggcacc gcgatgccta
    23761 atcagatgtc ggcggtgatc gcggccctgc gcgagggccg catccacaac ctcctgctga
    23821 tgggcaccaa catgctgtcg tcgttcgccg ctgcgggcgc ggtggccgag gggctggcgc
    23881 gcacgcggct ggtggtgagc tacgacctgt tcctgaatga caccgccagg cgctacgccg
    23941 atgtcgtgct gcccggtacg gcctggctcg aggagctggg ctgcaagatg acgcacacgc
    24001 acctgtacct gatggagccc gccctggcgc cggccggcga gacgcgatcg gtcaaccaag
    24061 tgttcaaggc gctgggcgag cggctgcagc tcgagggctt ctacccctgg gcctcggacg
    24121 aagcgctggt ggacgcgatc ctcgatcacc cgtgcacggg ccgcgccacg gtggcctcgc
    24181 tcagagcgcg cggcggcatc ggcgcattgc acatctcgca cgtggccaac cccacgctcg
    24241 aattcgatac cccgtcgcgc aagatcgagt tctactcggc gcgggcgcaa gcgctcggcc
    24301 tgccgccgct accctcgttc gacgcgcccg ccgcgaacgc ctacccgctg ccgctgacgc
    24361 agggccgcac gctgacgcac tttcacgcct tctacaacaa cgggcgcgag ctgccgacgc
    24421 tggcgcgacg cgacacgcac ccggaagtct ggctcgcgcc caccgacgcg aagcagcgcg
    24481 gcatcgagga cggcgccgcg atccgcgtgt tcaacgaccg cggctcgctc gcggcccgcg
    24541 cccacgtgac cgagcgcatg ccgccagggg ccctgtggct gcgggacggc tggccggggc
    24601 tcaaccagct gaccgacggc gccgcggtgc tgccggacga ggccgcggag atctttgcgt
    24661 tctccgcagg ccagtcgagc ttcgacgcgc gggtggaggt cgcgccggcc tagccgtgca
    24721 gcgactggta cttctcctgc agctgctccg gcgtctccac gaaatccgga ttggtcacga
    24781 tgcaatcgct caccgggcac accagccggc actgcggctc gtccttcgcc cccacgcact
    24841 cggtgcactt cagcggatcg atgacgtaga tcggattgcc cgcggtaatc gcatcgttcg
    24901 ggcactccgg cacgcaggcg tcgcaggcgg tgcaggtctc attgatcatc agggccatgg
    24961 aatcctcctc tcaggcggcg gcgcgcgcct gcagctcttt cagcttgtcg tgccggaacg
    25021 caccccacaa agccgcgccg atcgctccag catagatcga gtccgggtgg ctcgccacct
    25081 cgatacccgc gaccttctcg gtcaccagct cctcgcgcaa ggcggcgagc agcccgtcgt
    25141 ccagggccag cccacccgtc agcagcacct tgccgctctt gatgcccgtg actttcagca
    25201 gcttcaccag ccgcgaggcc atcgacaggt ggatgccttt caggatgtcc tgggtggcga
    25261 tgccgcgcga gaccatgttg atgacgtcgg tctccgcgag caccgcgcag atcgagctca
    25321 ccttctccgg gttctcggag gtcgtcgaca gccgcccgat ctcctccacc gcgaccccca
    25381 gatagcgcgc gatgttctcc aggaactgcc ccgacccgga ggcgcactgg ctggtcatct
    25441 tgtaggacag gaccttgccg cgctcatcca cgtagatggc gcggccgttc aaggcgccga
    25501 tgtccaccac cgcgctcacg ccgggcatca ggtacacgcc gccgcgcgcg tgcgtggtca
    25561 tggtatagaa gtgcccggtg gcgaacttga cgttctcgcc ttccccggtc gtcgcggtgt
    25621 aggcaacatc ctcggccgca agcttcctct cctcgagcac ttcgtcgtag gtctgtttcg
    25681 cgagctccat cgggtcgcgc cgccggatgc gctcgcagcg cttcgccagc cactcggtcc
    25741 gggcgccgtc cgagcgaaac agcgccgcct tcaccgcgcc ggacccgatg tcgatgccta
    25801 ccgtatggat gctcatgccg tctcccgctt ctcgatggcc ttgccctcct ccaccaccgc
    25861 gcgccaggcg aaggtcgcgc cgcccagagc cccggtgtag atcgagtcgg ggtcgatgtt
    25921 gatcttcagc ttgccgtagt tctcctcgat cagctcggtg agcgacttga ccgccgcctc
    25981 gttcttggcg acgccgccgg tgaacgtgaa ctcgttgcgc accccgcccg agcgcgccag
    26041 gatcgacagc gcgcgcagga tgatggcgcg gtgcagcccg gccatgatgt cttcgcgctt
    26101 ctcgcccagc gacagccggt cgcgcagctc ggcgccggca aacaccgtgc aggtggaatt
    26161 gatgcgcacc gtcttggtcg ctttcatggc cagaggcccc agctcgtgca ggcccatgtt
    26221 catctcgtcg gcgatgtagc cgagatagcg tccgcacccg gcggcacagc ggtcgttcat
    26281 ctggaacgac tccacgatgc cctgcggatc cacctggatc gccttggtgt cctggccgcc
    26341 gatgtcgagc accgtggccg tggacggata catcacgtgc gcaccgagcc cgtggcacag
    26401 gatctcggag cggatatgct ccttcgagaa cggcagccgc gcgcggccgt agccggtgcc
    26461 gaccacgtag gcttcctcca gcgccaggtc gagcacgccc tggatgggcg ccgagatctt
    26521 cgccttcttg cccgccgtct cgggcagcgc ccggtaggtg cgctccagag ctgcgcgcag
    26581 gttcttcgag atggacgcgt cgtacacgcg attctccacc tcgatgatcg agcgatcgaa
    26641 gacattgagc aggaagtcgt agcgcagccc cgcctccttc gccacctctt cgctgagcga
    26701 gtggtagcgg ctgccggcga tgtcgcggaa gaagtcgctc tttcttttcg ccccgggcgc
    26761 aaagagctca ggcgcctcct gccgcaggcg cgcgaacacc tgcacgagcg cctcgtcgac
    26821 cgccttctcc gcctcgccga agcgcgccgt cgccacgctc ctctcgcaca cgtcctcgag
    26881 gtcggcgagc tgcaccagga actgctcgag ccggaagtcg cgctcgagct gcgcgacgaa
    26941 gtcttcgagc ccgccgttga gcgcgccgct cgcagcgagc gcctcgcgaa acaggtgaaa
    27001 gcgcgccgaa agcagggctt cctgcttggc gatggtcgcc gcggtgtcgt agttcgagcg
    27061 cgagttggtg atgccgcgcc cgagcacgcg ttgctgctcg tcgatcacca ccgccttggt
    27121 ggtggtcgag cccaggtcga tgccgatgaa gcagcgcatg gcggtctcct cagtgcgcgg
    27181 cgggaaccac cggcggcagc cccgggcgtg cgccgccgcc ggcgccgcgc ttctgcctga
    27241 ccatctggaa gtagctctcc aggcggttct tgatgttggc cgcggagaag tagcgcggat
    27301 ccaccaggtc cgactcgatg aacgcggcgg gcttgccggt gcgccgctcg acctcgcgca
    27361 ggatcagcag ctgtcccgcg gagaagctgt tgcagctctt gatcgagttg atcagcaggc
    27421 cgtcggcctg atactcgtcc agataccggc agatcatgtc gatcctctgc gggaaattca
    27481 ggttggtgta gcagcccagg caatactcgg cgagcgactc gagcggatgc tccgggtcgt
    27541 ggcgaaagcc gaagtcgtac acgccgccga ccttggcgta ggtcgaggcc accaccaccg
    27601 cgccctcgtc gtagaacatc ttccagaact cgcgaaagct cgtccagttg ggcggcccct
    27661 cgaccaccag gcggtatttc tcgcccggat gctcgccgtc gggtgtcacc ggccccttgc
    27721 cctcgcgcat gcgcagctcg atctcgccgc gcagcacccg gtagtactcg ctcgcctcgg
    27781 gcatgccgcg gaacgcggtg aagatcgggc cgatgtagta caccgcgccg aagtagccgt
    27841 cgatcggcga aggcttgtgc ttggccgact gcagcgtcca caccaggtcc tcctcggcct
    27901 tcaccgactc gcgcatgtac tggcgcagcc ggtcgatgtc gaacttgacc ccggacacct
    27961 tctccagcgt cgggatgacc ttttccttga gctgcttgac gacgtagtcg cgcatgttcg
    28021 gcgagatcct gccgtcgccc tggtaaggca cgtgcagcat caccgtctcg cagtcgtact
    28081 tctggcggat cagctcgaac cacttcatga acgtgaagca gccggtgtac gaaagcagca
    28141 gcacgtcggg cgtgggcagc ggttcgcccg taggccccac cggcccctgc agcatcatgc
    28201 cgaggtcggc cttgacgtag gtgcagacgt cctcggagtg cccgtcgcgc tcggcgtcca
    28261 tgatcatctt gccggatttc ttgcgcatgc cgttctgcag cgcgttggtc tcgggcaggc
    28321 tgcgcgcgaa atcgaagcac atcaggagct cgttcaggtt tccgggcacg aaggtcgccg
    28381 agaccttgcg gttcgtctcg tgcgccgtgg cgagctcgtg gtagttgcgg ttgatgagct
    28441 ccttttgcag cagcatggcg tcttccttct tcgcctgcgg cggcttcttc ggggtgctgg
    28501 atccgggcac gatctggatc ggtgtgctca tgcggcgctc cacaatttga tcgagtcggc
    28561 gaaggtgccc gcctgctcgc ggatcggggc catctggccg gtgttctcgg cgtacttgaa
    28621 cgcggtatag ggcaccttgt gcttcgccag cacctcctgc agcatcggcc gctcaaggag
    28681 cgccgggtcg cagaacgacg ccgcggcgaa caccacgccc tcggcgccgc gctccttgat
    28741 ctgcttgagc aggaacttgc ccttgtcttc cttcttctcg tcgtacttgg acgaggtggc
    28801 ggccgagcgg tgcaggaacg ccttcgacag ctcttcgagc ggctcgccgg tcaccggcac
    28861 gtcgtccagc agccagctct ggatgagctg gaagtcgtcg tccaccacgt agcagcccga
    28921 catctcgatc gacttgatga ggctcaacgg cggctgctcg cagaacgagc cggtgagcac
    28981 cacgcgcgcg ttgtcgcgcc gcggccgttc ctcggcgcgc gccgccgcga ggtacgcgcg
    29041 caccagcgcc gtgtgctctt cgggcggcag cacgcagccc gcgcgcagga ccagatacag
    29101 ctcggaagtc ggcgcctgcc agggcgccgc ggcgcggtag gcgtagagct cgcgcaccgc
    29161 cttgcggttc tcgttgtaca cggcgatcga ggcgcgcagc gcgtcgtcgg tcacctccct
    29221 gccggcgagc ttgccgagat cggcgcgcaa ctgctgcatc tcgtgcacgt agaactcgcc
    29281 gccgatggcg tcgtcgtagt tctgcggcac gtcgaagtac ttgacgtact tgtccttgaa
    29341 catgatctgc cacatgcccg agaggttgcg gatcacgtcg cagatcgacg ggaacagcat
    29401 gccgtcgagg acgtcgagcc gcccggtgag gccgagctcg agcgtcgagc gcgggatgcg
    29461 gcagatgtag ctctggtagt aggcgtcgcc ctggatcacc tcgagctggt cgccgccgcc
    29521 gaagatcccg accggcagca tgcccgcggc gtggatgatc tcgcgcggca cgtagatcgg
    29581 catgtagccg atggctttcc tgcctggtac ggccgccttc cagtccttca ccgcaccgaa
    29641 gcgcaggtcc tcgaacaggc gctcgcagcg cgccacgatc tcctgcgcgt tcatcggtgc
    29701 tcccatttcg gcgcgcgctt ttccatgaag gcggcgaggc cttcacgggc atcgcgcgtg
    29761 gccatcagct tatccaggta cagggcctcg acgtcgtcga gccgcgcgcg cgccaggtcc
    29821 gcgaacggct cgcgcgccgc gcgcaccgcg tgcgcgagcg cggcagcgct ctttggcgcc
    29881 aggtgctcgc cgaagtacgc ggcggccgct gcggccgggt cctccgcgac ctcgttggcg
    29941 aggccccagg cgagcgcggt gggcgcgtcg atcgagcggc ccgagtacag caggtcctcg
    30001 gcgcgcgcct ggccgatgcg cagcggcagc agcaccgacg cggcgggcgc gaacacgccg
    30061 agcttcattt ccggctggcc gaagcgggcg tcgggcgcgg caaacagcag gttgccggcg
    30121 agcgcgaact cgagcccgcc gcccaggcac tggccgcgca cgcacatgag tatcggccgc
    30181 ggccactcca gcatcgcgcc gagcaggcgg tgcaggccgg cgagcatctg ctcgcaggcg
    30241 cccggcaggt gctcctcgac gctcgcgccg aaggaaaagt gcgggccttc gtggtcgacc
    30301 agcgccgcga gcagccccgt attgtcgcgg tattcggcga aggcgtcaga cagcgccgcg
    30361 atcatcgccg cgtcgatcag gttggctttc gggcgcgcca gccgcagccg cagcagcgcg
    30421 ccgtcgcgct cgagccaggc cttgagcggc gtctcgctca cccgcgcttc gccttcggct
    30481 ggatggcgtc gtgcagcgcg cccacccagc cctcgcccgc ggcgagcttc tgcctgagca
    30541 gcacgaagtc cacctcgcgg tcgtccttcg ggcccgagtt gaacgcggtg aagccggcgc
    30601 gtgcctcggt catcatgttg agcgcgagcc aggcgcgcgc gtcctccttg ttgcggttcc
    30661 aggcctcgag cttgggtttc ctgagctctt ccagcgtctt ggtggtgcag tcggggaagg
    30721 tgagcaggat cttcgcgcac agcgcctcga cgcattcgtc cagcttcgag aggtccacgg
    30781 cgccctgctt catgagtgcc ttgccctgcg cgagcgcctc gcccgtcttc ggccggccaa
    30841 agaccaggcg cccgaactcg tcgtacacgc gctgcgtctc gaccagcggg ttggcgacga
    30901 acttgccgtc gaccttgagg gccggaacga tgtcggtgag cacccccata tggtaggcct
    30961 cgtgcgcgga gaacggctcg cacagcacgc aggctgccat ggcgcgctcg gcgccggcaa
    31021 tcaccggcag gaagtcggtg gcgccgccga tcggcgccga gccgtgcttg gggccggcct
    31081 ggccgaagcg cgccaggtcc tgggcgaccg agaaatcgca ggccatgccg atctcctggc
    31141 cgccgccgat gcgcatgccg ttgacgcggc agatcacggg cttgtcgcag gcgaggatcg
    31201 ccgacaccat gtcgttgaac aggcgcatgt attgccggta ctcctgcggg tgcccggcgt
    31261 agtactcggc gtactccttg gtgttgccgc cggtgcagaa cgccttgtcg cccgcgccgg
    31321 tgaacaccac gcagttcacg tcgcgcgcgt tggacgcggc gcgaaaggcg aggatcaccg
    31381 ccttcaccat ctcggtggtg tacgagttgt actgcgtcgg gttgtcgagc cagatccagg
    31441 cgttatgcag tcccggcacc tccttgccgt cgggcgtctt cgccgcgcgc ttttcgtagc
    31501 gcacgccgcg ctcggcgagc gccggcaggt cgtggtcctt gaggaacttc gcgtccggct
    31561 tgcccatgtc gacccctttc tcctgaaatc ggtatccaaa taccgaatta gaccctttct
    31621 acccccggga aaagcgcccc ggtttgatcc atctcaaggt ttgggcaggg ccttggcccc
    31681 caaatagcgc catgctcgcc cacgcctcgg tttccctggc gctcgccggc tccggcggtg
    31741 ccggcgtcat gaccgccggc aatatgctgc tggaggccgc ggcgcaggcc ggctactacg
    31801 gcctgatgac gcgcaccagc ggcccgcaga tccgcggcgg cgaggccgcg gcgatgctgc
    31861 gcgtgtcgac cttccccgga gacggccagg acgacgcttt tcacgtgctc gccgccatcg
    31921 actggcagaa cgtgcagcgc tttgccgccg agctgccgct cgggccgcag agcctcatcg
    31981 tcggcgatcc cgcccacggc gcgccgcccg aggtgttcct gcgcggcggc gcgcgctatg
    32041 caccgctgcc gctgaagaag ctcgcgggcg aggtcgctgg gggctggccc aacatggtcg
    32101 cgctcgggct gctcgcggcg ctgctcggcc tgcccgaggc ggcgctcgag gccgcggtgc
    32161 gcaagtccat gaagaagggg ctcgacgcgg ggctcgcggc cgcgcgcctc ggcagggcgc
    32221 aggcggccgg gctgccgccc tgcacgctcg ccgcgccgcc ggcgcacgcg ccgcgctggc
    32281 tggtcaccgg caacgaggcc gccggctacg gcgcgttgcg cggcggcgtg cgcttcgtcg
    32341 ccgcctatcc gatcaccccg gcaaccgagc tgctcgagta cctcgcgccc gcgctcgccc
    32401 aggtcggcgg cacgctggtg caggccgagg acgagctcgc ctccatcaac atggcgctcg
    32461 gcgcctccta cggcggcgtg cccgcgctca ccgccacctc gggcccgggt ctgtcgttga
    32521 tgatcgaatc gctgggcctg ggcgtcgccg cggaaatccc ggtcgtggtc gtggacgtga
    32581 tgcgcaccgg cccctcgacc ggcatcgcca cgaagagcga gcaggccgac ctggggaccg
    32641 cgctctacgg gctgcacggc gacgcgccgc acgtggtggt cgcgcccaac tcgctcgccg
    32701 actgcctcgc cgccaccgag tgggccgtgg gcctggcgga atcgctgcag gtgccggcga
    32761 tcgtgctctc cgaccagtac ttcggccagg cgcgcgcagt ggtggatgcg ccgagcgcgt
    32821 cgagcggcgc cgtcgcccgg ctccgcgccg aagcggccgg gggctaccag cgctacgcgg
    32881 tgaccgacgc cggcgtctcg ccgatggcga ttccgggcgc gcccggcctc acctacaccg
    32941 ccgacgggct cgagcacaac gaacgcggca cgccctcgtc gcaggcgagc gaccatctcg
    33001 cgcagatgga caagcgcgcg cgcaagctcg cgcgactcga cccgggcgag cactgggcga
    33061 gcgtcgaggg cgagggcgac actgcgctca tcaccttcgg ctcgtgcacc ggcccggcgc
    33121 gcgaagcgct ggcgcgaaac ggcaacggcg tgcgcctggt gtcgctgcgc ctgctcgcgc
    33181 cggcgcagcc cgagcgcctg aagaaggcgc tcgaaggcgt caagcgcgtc ctggtgctgg
    33241 agcagaacca cagcggccag ttcctgaggt acctgcgcgc cgagtacgag ctgccgggcg
    33301 aggtccggag cctcaggcgc cccggcccgc tgccgttccg ccccggggaa atccgcgatg
    33361 cgctcgggca atggagccgc acatgaacgc gcccgccgac gcgacgctct gcgcgccgaa
    33421 ggacttcaag tccgacacca agccagtgtg gtgcccgggc tgcggcgact attcggtgct
    33481 cgccgcgatc accaaggcgc tcgccgcgct caacctgcgc cccgagaacg tcgtcgtcgt
    33541 ctccggcatc ggctgctcct cgcgcatccc ggcctacacc gcgtgctacg gctttcacgg
    33601 cgtgcacggg cgcgcgctgc cggtggccac cggcctgaag gccgcgcgcc cggatctcac
    33661 ggtgctggtg gcgagcggcg acggcgacgg ctactccatc ggcggcaacc acttcctgca
    33721 cgcctgccgc cgcaacatcg acctcaccta catcgtgatg gacaaccacg tctacggcat
    33781 gaccaagggc cagccctcgc ccaccaccga gcccggctgg tcctccaagc tcgcgcccgg
    33841 cggcacgggc ctgaaggaat tccacccgct ggtgatcgcg ctcgcgtccg gcgccaactt
    33901 catcgcgcgc gcgtactcgg gcgacccgaa cggctgcgcc gcgctgatcg ccgaggcggt
    33961 gcgccatccg ggcttttcct tcgtcgaggt gctctcgccc tgcgtcacct tccgccccga
    34021 gcagcgcgag tggaagacgc tggcgcaccc ggccccggtc gcgcccaccg acgacccggg
    34081 gcgcgccgcg cggcgcctga tgaccgacga cggcttaaac atcggcgtgc tctaccaggg
    34141 caagcgcgcg ccttatgcgc gcgcatccgg caccgaacaa accgtggagg cgctcgcatg
    34201 ccagttccga ctctagagga gttcatggcg cgcgcctacg ccatggagct cgaggcgcac
    34261 gagcgctacg cgctgttcgc cgagcagctc gacacgcaca acaaccgcgc ggtggccgag
    34321 atgttcggca agctggccag aatcgaggcg ctgcacgcca agcgcatcct cgaggaaatg
    34381 ggctggccga gcctgcccac gctgccgccc gcctatgcct gggacacgcc cgaggcgccg
    34441 gaaaccgcgc cgccggacgc ggtgcactac ctgatgcagc cctaccacgc gctcgagatc
    34501 gcgctgcgct gcgagctgca ggcgcaaaag tacttcgagg acatcgccgc cgcgagcggg
    34561 gtgccgccgg cggtgaagaa ggccgcgctc gagatggccg acgaggagcg cgagcacgtg
    34621 agcctggtcc gcgactggat gaagcgcgtg ccggtgccgc cggacggctg ggacgaggac
    34681 cccgacccgc cgcgcgtcac cgaatgagag tgctgcaacc gcgcggctgg gcgaaccccg
    34741 tcggctacgc caacggcatc gaggcgcgcg gccgcgtcgt cttcatcgcc ggacaggtcg
    34801 gctgggacgc ggcgcagaaa ttccattcgc aggacctcgc gccgcagttc gagcaagcgc
    34861 tcgcgaacgt gctcgcggtg ctcgcggaag cgggcgggcg cgccgagcac atctgccgca
    34921 tgaccgcgta ctgctgcgac aagccggcgt acctcgccgc gcgcccggcg ctcgggcgca
    34981 tctggcgctc gctcatgggc cggcacttcc cggcgatgag catgatcttc gtcgccgacc
    35041 tgctcgatgc gcccggcaag atcgagctcg aagccaccgc tgtgattccg gaggagacaa
    35101 catgaaggga ttgaaaggca agaccgccat cgtcaccggg ggcgccgccg gcatcggcgc
    35161 cgcgatcgtc gagcgcttca gggccgaagg cacgaaggtc gtggtgttcg acctgaaggg
    35221 ctcgccgagc ctcgacatca ccgattacga ggcggtgaag aaggcggtgg ccgcggcggg
    35281 gccggtggac atcctcgtca acaacgccgg ctgggacatg ttcaagccgt tcctcgccac
    35341 cgatcccgcc ttctggcaga agatcatctc gatcaacctc gtcgggccga tgaacctgct
    35401 gcactgcgtg ctgccgggca tggccgagcg cggcggcggc aaggtggtga acatcgcctc
    35461 cgacgcgggc cgcgtcggct cctcgggcga ggcgccctac tcggcgtgca agggcggcat
    35521 catcgccatg accaagacgc tcgcgcggga gctggcgtcc aagggcgtgc ggctcaacac
    35581 ggtgtgcccg ggcctcaccg agacctcgat gctcgaagcc ttcatgcagg gcgccggcaa
    35641 cccggacaag ctgcgcgagg cctaccgccg cgcggtaccg atcgggcgcc taggcaagcc
    35701 cgaggacatc gccggcgcgg tgctgtttct cgccagcgac gacgccgaat tcatcacggg
    35761 gcagacgatc agcgtctcgg gcggcctgac tatgcacggc taggccgcgt gcccgccgcg
    35821 tggcggcgga tcttcttgcg gcgctcggcc tcggcggtga actcctcgag atccgggtcg
    35881 agggcatggc gcgcggagat gacgccgacc ggacggcgtt tctcgagcac gggcaggtgc
    35941 cggaagccgt gctcgtgcat gacgagcagc gcgtagccga gggtctcgtc cgcggcgacg
    36001 gtcttcgggt cgtgcgtcat gacctcggcg agccgggtcg ccgccgggtc gcgtccctcg
    36061 gccatgacgc gattgagcgc gtcgcgctcc gtgaagatgc cgaccagctg ctcgccgcgc
    36121 accaccagca cggcgccgac gtggctca
//
LOCUS       DBNHKE010000213         7849 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_779989, whole genome shotgun sequence.
ACCESSION   DBNHKE010000213 DBNHKE010000000
VERSION     DBNHKE010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_779989"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1055)
                     /gene="cofD"
                     /locus_tag="AC16O9_14530"
     CDS             complement(87..1055)
                     /gene="cofD"
                     /locus_tag="AC16O9_14530"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR6517868.1"
                     /translation="MGTVVALGGQVGGAKLAEGLYRLRGKDLAVIVNTGDDFDYLGLH
                     VSPDIDTMLYALAGIADAAKGWEPAGETFASHAMLMRLRGPGAVPLGDRALALQLLRA
                     EALRAERTPTEITQDLAQRLGIEARVLPMSDDPVRTVVNTDSGAVSFQDYFVDLNCEP
                     AVRSFQYGGADEARLSDEVLDALHAHDLEAVVLGPCNPYHTMHPILAVNGMREALRKR
                     GAPVVAVSPIIGGRALQGSAAKMMRELGREVSARAVAMEYYKLIDGIIVDSADEALVE
                     GIRASGIHALAVPTLMRTPEDRLKLARAALEFARSVRLRKEIAADA"
     gene            complement(1056..1739)
                     /locus_tag="AC16O9_14535"
     CDS             complement(1056..1739)
                     /locus_tag="AC16O9_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6517869.1"
                     /translation="MPSRHRMPGKIFRFGPTPEHRAQHLFVFLHGSGATSQSMIPIAF
                     RFQARFPSAALVVPSGFARSEVDHDAQQWYSSRGLNDDNRAERVAAILPRVEALVRRE
                     QTNCRVPMERTVLIGFSQGGTVALEAVKQLPELAGAVVAYAARFARLPKSGTRIGSRV
                     HLVHGEYDAIVSRVHAERAARTLAALHVPVTLDIVEHLGHALTHSAICLGSLRLLQGI
                     YEGRRATLH"
     gene            complement(1823..4669)
                     /gene="uvrA"
                     /locus_tag="AC16O9_14540"
     CDS             complement(1823..4669)
                     /gene="uvrA"
                     /locus_tag="AC16O9_14540"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6517870.1"
                     /translation="MDSHGTLRIRGARTHNLKNLSIELPRNRLVVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSAYARQFLQLMGKPDVDLIEGLSPAIAIEQKAGSHNPRSTVG
                     TVTEVHDYLRLLFARVGTPYCPDHGLALQSQSVAQMVDHVLALPADTPLMVLAPVVAG
                     RRGEQADLLDELRAQGFTRVRVDGKVHELDAAPKLARHAKHSVDVVVDRLKVRPEAKQ
                     RLAESFETALRHADGRAVAVETDSGREHLFSAKFACPACSYALAELEPRLFSFNNPAG
                     ACPRCDGLGAVEFFDPKRVVAHPNLSLASGAIRGWDRRNHFYYTLLQSLAAHYGFDIE
                     TPWEALADDVQDRVLNGSGSERIAFRYPNERGRTTVKEHAFEGVLPNLERRYRETDSS
                     VVREELAKYRNTRTCPECGGARLRREARHVRVADTTIHELSCWPLRQSQAFFQGLALP
                     GARAAVAEKIVHEISGRLGFLVNVGLDYLTLERAAQTLSGGEAQRIRLASQIGSGLTG
                     VLYVLDEPSIGLHQRDNARLIETLRHLRDLGNSVIVVEHDAETILAADHVVDMGPGAG
                     EAGGNVVAEGPPAAILKSEQSLTGQYLSHRLSIEVPRLRRRRNPRQQLRISGASGHNL
                     KQVAFELPVGLFVCVTGVSGSGKSTLVNETLYRALARHLYGSAEEPAPHEAVSGLEHF
                     DKVVSVDQSPIGRTPRSNPATYTGLFAPIRELYAGVPEARARGYDAGRFSFNVKGGRC
                     EACQGDGLVKVEMHFLPDIYVPCDVCHGTRYNRETLEIRYKGKTIHDVLQLTVREAHA
                     FFGAVPVVARKLQTLLEVGLGYVQLGQAATTLSGGEAQRVKLSLELSKRDTGRTLYIL
                     DEPTTGLHFHDIKLLLEVLHRLRDAGNTVVVIEHNLDVVKTADWIVDLGPEGGDGGGL
                     IVAQGTPEAIARSGDSHTGRYLKAVLEVPRRRAA"
     gene            4762..5964
                     /locus_tag="AC16O9_14545"
     CDS             4762..5964
                     /locus_tag="AC16O9_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517871.1"
                     /translation="MSAQELRAGASLAGIFGLRMLGLFLILPVFAVHAPQLAGGDNLV
                     LVGAALGAYGLVQALLHVPFGMASDRWGRKPVIVVGLVIFAGGSFLAAGAEHIWTAII
                     GRGLQGAGAISAVVMALAADLTRDEHRVKTMAMIGATIGFSFALSLVAAPSLYHLIGM
                     GGLFAFTGALALAGIGVIAGLVPDAPRRPRVAAAPGALRRALLDPQLLRLNAGIFVLH
                     LVQMAMFVVVPPLLVASGLPLAAHWKIYLPVVLASFVVMLPAVLHADRRNAGRGVMLG
                     AIALLLLAEGGLAFSGPRIAVLALLMLAFFSAFNVLEALIPSLVSRLVPAQARGTAIG
                     VYNTAQTLGLFCGGLLGGWIAGRYGPAAVFGACAALALAWLGIAYGMRPVNRIGGESF
                     TMSTVKGR"
     gene            5967..6428
                     /gene="ssb"
                     /locus_tag="AC16O9_14550"
                     /pseudo
     CDS             5967..6428
                     /gene="ssb"
                     /locus_tag="AC16O9_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            6484..6813
                     /locus_tag="AC16O9_14555"
     CDS             6484..6813
                     /locus_tag="AC16O9_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR6517872.1"
                     /translation="MPIYEYRCTDCGFQDEYLQRVSEPPLTDCPSCGKPAFRKLVSAA
                     GFQLKGSGWYATDFKGAGKAAAKADAQGAAKPESKTEAKSEAKAEAKAEAKSAPTKTP
                     AAANSDS"
     gene            6876..7472
                     /locus_tag="AC16O9_14560"
     CDS             6876..7472
                     /locus_tag="AC16O9_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR6517873.1"
                     /translation="MRKYFITGLLIWIPLVITIWVLDLIVTTLDQSLHLLPPQWRTES
                     FLGFHVPGLGVILTLVIVFVTGVLATNFLGARLVNLWNEILHRIPVVNSIYSSVKQIS
                     DTLFSSSGQAFRKALLVQWPREGMWTIAFLTGRPGGDVVRHLPGDYVSVYVPTTPNPT
                     GGYFVMMARKDVIELDMSVDAALKYIISMGVVAPGPGR"
     gene            7481..>7849
                     /locus_tag="AC16O9_14565"
     CDS             7481..>7849
                     /locus_tag="AC16O9_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR6517874.1"
                     /translation="MRTHYCAELSAALVGKQVTLAGWAHRRRDHGGVIFIDLRDREGL
                     AQVVCDPDRAQAFRAAEGVRSEFVLQVTGRVRARPEGTVNPNLASGAVEVLAESVEIL
                     NPSLPPPFQIDEDNLSESVRL"
BASE COUNT         1132 a         2681 c         2796 g         1240 t
ORIGIN      
        1 cgcctgatgg acttcctcgc gcgcatccac gcgcgccccg cctaccgccg cgccatcgac
       61 cagggcggcg agtacgcgct gctgcgctag gcgtcggccg cgatttcctt gcgcaggcgc
      121 accgagcgcg cgaactcgag cgcggcgcgg gcgagcttca ggcggtcttc gggcgtgcgc
      181 atgagcgtgg gcaccgcgag cgcgtggatg ccgctcgcgc ggatgccttc gaccagcgct
      241 tcgtcggccg aatcgacgat gatcccgtcg atcagcttgt agtactccat cgccaccgcg
      301 cgcgccgaca cctcgcggcc cagctcgcgc atcatcttcg ccgccgagcc ctgcagcgcg
      361 cgcccgccga tgatcgggct caccgcgacc accggcgcgc cgcgcttgcg cagcgcctcg
      421 cgcatgccgt tgacggcgag gatcgggtgc atcgtgtggt acgggttgca ggggccgagc
      481 actaccgctt cgaggtcgtg cgcatgcagc gcgtcgagta cctcgtcaga gaggcgcgcc
      541 tcgtcggcgc cgccgtactg gaagctgcgt acggcgggct cgcagttcag gtcgacgaag
      601 tagtcctgga agctcaccgc gcccgagtcg gtgttgacga cggtgcgcac cgggtcgtcg
      661 ctcatcggca gcacgcgcgc ctcgatgccg aggcgctgcg cgaggtcctg cgtgatctcg
      721 gtcggcgtgc gctcggcgcg cagcgcctcg gcgcgcagca gctgcagcgc cagcgcgcgg
      781 tcgccgagcg gcacggcgcc gggaccgcgc aggcgcatca gcatggcgtg gctggcgaag
      841 gtctcgccgg cgggctccca gcccttggcg gcgtcggcga tgccggcgag cgcgtagagc
      901 atggtgtcga tgtcgggcga gacgtgcagc cccagatagt cgaaatcgtc gccggtgttg
      961 acgatcaccg cgaggtcctt gccgcgcagg cggtacagcc cctcggcgag cttcgccccg
     1021 ccgacctggc cccccagtgc gacgacggtg cccattcagt gcagcgtggc gcgccggccc
     1081 tcgtagatgc cctgcagcag gcgcagcgag ccaaggcaga tggcgctgtg cgtgagcgca
     1141 tggcccaggt gctcgacgat gtcgagcgtg accggcacgt gcagcgcggc gagggtacgc
     1201 gccgcgcgct cggcgtgcac gcgcgacacg atcgcgtcgt actcgccatg caccaggtgc
     1261 acgcgcgagc cgatgcgcgt gccgctcttc ggcaggcgcg cgaagcgcgc cgcgtaggcg
     1321 accaccgcgc cggcgagctc ggggagctgc ttcaccgcct cgagcgccac cgtgccgccc
     1381 tgcgagaagc cgatcagcac ggtgcgctcc atcggcacgc ggcagttggt ctgctcgcgg
     1441 cgcaccagcg cctcgacgcg cggcaggatc gccgccacgc gctcggcgcg gttgtcgtcg
     1501 ttcaggccgc ggctcgagta ccactgctgc gcgtcgtggt cgacctccga gcgggcgaag
     1561 ccgctcggca ccaccagcgc cgcgctcggg aagcgcgcct ggaaccggaa cgcgatcggg
     1621 atcatcgatt gggaggtggc gccgctgccg tgcaggaaca cgaacaggtg ctgggcgcga
     1681 tgctccgggg tcggcccgaa gcggaagatc ttgccgggca tgcggtgtcg ggagggcaag
     1741 gccattctcg ccccattgcc cgttgcctgt aaaccacttc agcccagccc cttttccggg
     1801 cgggcggcgc gtttgcaccg cgtcaagccg cgcgccggcg tggcacctcc agcaccgcct
     1861 tgaggtagcg cccggtatgg ctgtcgccgc tgcgcgcgat cgcctcgggc gtgccctgcg
     1921 cgacgataag cccgccgccg tccccgcctt cggggccgag gtcgacgatc cagtcggcgg
     1981 tcttgaccac gtccaaattg tgctcgatca ccaccacggt attgccggca tcacgcaggc
     2041 ggtgcagcac ctcgagcagc agcttgatgt cgtggaagtg cagcccggtc gtgggctcgt
     2101 cgaggatgta gagcgtgcgg ccggtgtcgc gcttggacag ctcgagcgag agcttcacgc
     2161 gctgcgcctc gccgcccgag agcgtggtcg ccgcctggcc gagctgcacg tagcccaggc
     2221 cgacctcgag cagcgtctgc agcttgcggg cgacgaccgg gacggccccg aagaaggcat
     2281 gcgcctcgcg caccgtcagc tgcagcacgt cgtggatagt cttgcccttg tagcggattt
     2341 cgagcgtctc gcggttgtag cgcgtgccgt ggcagacgtc gcaaggcacg tagatgtcgg
     2401 gcaggaaatg catctccacc ttgaccaggc cgtcgccctg gcaggcttcg cagcgcccgc
     2461 ccttcacgtt gaacgagaag cgcccggcgt cgtagccgcg cgcgcgcgcc tccggcaccc
     2521 cggcgtagag ctcgcggatg ggcgcgaaca gcccggtata ggtggccggg ttggagcgcg
     2581 gcgtgcgccc gatcgggctc tggtcgacgc tcaccacctt gtcgaaatgc tccaggccgg
     2641 acacggcctc gtgcggcgcg ggctcctcgg cgctgccgta caggtggcgc gcgagcgcgc
     2701 ggtacagcgt ctcgtttacc agcgtggact tgcctgaacc ggagacgccg gtcacgcaga
     2761 cgaacagccc gaccggcagc tcgaacgcca cctgcttcag gttgtggccg ctggcgccgg
     2821 aaatgcgcag ctgctggcgc gggttgcgcc gccgtctcaa gcggggcacc tcgatgctca
     2881 ggcggtgtga caggtactgc cccgtaagcg actgctcact tttgagtatc gcggcaggcg
     2941 gaccttcggc gacgacgttg ccgccggctt cgcccgcgcc ggggcccatg tccaccacgt
     3001 gatccgcggc caggatcgtc tccgcgtcgt gctcgacgac gatcaccgag ttgcccaggt
     3061 cgcgcaggtg ccgcagcgtc tcgatgagcc gggcattgtc gcgctggtgc aagccgatcg
     3121 aaggctcgtc gagtacatag agcacgccgg tcaggccgga gccgatctgg ctcgccaggc
     3181 gaatgcgctg cgcttcgccg ccgctaagcg tctgcgccgc gcgctcgagg gtaaggtagt
     3241 cgaggccgac gttgacgaga aagcccaggc ggcccgagat ctcgtgcacg atcttctcgg
     3301 cgaccgcggc gcgcgcgcca ggcagcgcga ggccttggaa gaacgcctgc gactggcgca
     3361 gcggccagca cgacagctcg tggatcgtgg tgtcggcgac gcgcacatgg cgcgcctcgc
     3421 ggcgcaggcg tgcgccgccg cactccgggc aggtgcgcgt attgcgatac ttggccagct
     3481 cctcgcgcac cacgctcgag tcggtctccc ggtagcggcg ctcgaggttc ggcagcacgc
     3541 cctcgaaggc gtgttccttc acggtggtgc ggccgcgctc atttgggtag cgaaaggcga
     3601 tgcgctcgct gccggagcca ttgagcaccc ggtcctgcac gtcgtcggcg agcgcctccc
     3661 acggcgtctc gatgtcgaaa ccgtagtgcg cggcgagcga ctgcagcagc gtgtagtaga
     3721 agtggttgcg ccggtcccag ccgcggatgg cgccgctcgc caggctgagg ttgggatggg
     3781 cgaccacgcg cttgggatcg aagaactcga ctgcgcccag gccgtcgcag cgcgggcagg
     3841 cgccggccgg gttattgaac gaaaacaggc gcggctcgag ctcggcgagc gcgtagctgc
     3901 acgccgggca ggcgaatttc gccgagaaca ggtgctcgcg gcccgaatcg gtctccacgg
     3961 cgacggcgcg cccgtcggca tggcgcagcg ccgtctcgaa cgattcggcc aggcgctgct
     4021 ttgcctccgg ccgcaccttg agccggtcta cgaccacgtc caccgagtgc ttggcgtgcc
     4081 gggcaagctt cggcgctgcg tccagctcgt gcaccttgcc gtccacccgc acgcgcgtga
     4141 agccctgcgc gcgcagctcg tcgagcaggt cggcctgctc gccgcggcgt cccgccacca
     4201 ccggcgcgag caccatgagc ggcgtatccg cgggcagggc cagcacgtga tccaccatct
     4261 gcgccaccga ctgcgactgc agcgcgaggc cgtggtcggg gcagtacggc gtgccgacgc
     4321 gcgcgaacag cagccgcagg tagtcgtgga cctcggtgac cgtgccgacc gtggagcgcg
     4381 gattgtggct gcccgccttc tgctcgatgg cgatcgccgg cgacaggccc tcgatcaggt
     4441 cgacgtcggg ctttcccatc aattgcagga actggcgcgc gtaggcggaa agcgactcca
     4501 cgtagcggcg ctggccctcg gcatacagcg tgtcgaaggc gagcgaggac tttccagagc
     4561 cggacaggcc ggtgatcacc accaggcggt tgcgcggcag ctcgatgctc aggttcttca
     4621 agttgtgcgt gcgcgcgccg cgaatgcgca gagtgccgtg ggaatccatc gcgccggttt
     4681 ggcaaaagta aacttgctac tatagcgagt tccggatcac cgtttccagc ctttctttcc
     4741 tccgtaatgg atgcccagcg tatgagcgcg caggagctgc gggcgggcgc ctcgctcgcc
     4801 ggcatcttcg gcctgcgcat gctcgggctg tttctcatcc tgccggtgtt cgcggtgcac
     4861 gcgccgcagc ttgcgggcgg cgacaacctg gtgctggtgg gcgccgcgct cggcgcgtac
     4921 ggcctggtgc aggcgctgct gcacgtgccg ttcggcatgg cctcggaccg ctggggacgc
     4981 aagcccgtca tcgtggtcgg cctggtgatc ttcgccggcg gcagctttct cgccgcgggc
     5041 gccgagcaca tctggacggc gatcatcggc cgcgggctgc aaggcgcggg cgcgatatcc
     5101 gcggtggtga tggcgctcgc cgccgacctg acgcgcgacg aacatcgcgt gaagacgatg
     5161 gccatgatcg gcgccaccat cggctttagc ttcgcgctgt cgctggtggc ggcgccgtcg
     5221 ctctatcacc tcatcggcat gggcggcctg ttcgcgttca ccggcgcgct ggcgctggcg
     5281 ggaatcggcg tgatcgcggg gcttgtgccc gacgcgccgc ggcggccgcg tgtcgccgca
     5341 gcccccggcg cgctgcgccg ggcgctgctc gatccgcagc tgctgcggct caacgccggc
     5401 atcttcgtgc tgcacctcgt gcaaatggcg atgttcgtcg tggtgccgcc actgctggtc
     5461 gcttccggcc tgccgctcgc ggcgcactgg aagatctacc tgccggtggt gctggcatcg
     5521 ttcgtcgtga tgctgccggc ggtactccac gcggatcggc gcaatgcggg gcgcggggtg
     5581 atgctgggcg ccatcgcgct gctgctgctg gccgaagggg gcctcgcgtt ctccgggccg
     5641 cgcatcgcgg tgctggccct gctgatgctc gcgttcttca gcgccttcaa cgtgctcgag
     5701 gcgctcattc cttcgctcgt gtcgcggctc gtaccggcgc aggcgcgcgg cacggcgatt
     5761 ggcgtctaca acaccgcgca gacgctgggc ctgttctgcg gcggccttct cgggggctgg
     5821 attgcggggc gctacggccc ggccgcggtg ttcggggcgt gcgctgcgct ggcgctagcc
     5881 tggctcggca tcgcctacgg catgcgcccg gtcaaccgga tcggcggtga gtcgtttacg
     5941 atgagtacgg tcaaagggag gtagccatgg catcggtcaa caaggtcatc gtagtgggca
     6001 acctgggcgc ggatccggaa acgcggtacc tgccgagcgg cgaagcggtc accaacatcc
     6061 gcgtcgccac caccgaccgc tggaaggaca aggcgagcgg cgagatgaag gaggccaccg
     6121 agtggcatcg catcgctttc ttcgggcgcc tggcggagat cgcgggcgag tacctgaaga
     6181 agggctcgca ggtgtacgtc gagggcagcc tgcgcacgcg caagtggcag gacaaggagg
     6241 ggcacgaccg ctacagcacg gagatccgcg gcgacgtgat gcagatgctt ggctcgcgcg
     6301 ccggggccgg cgagccgcgc gcggccccgg cgggcgctga cgccggtgat gcgcgcggcg
     6361 agcctgcggc gcggccggcg aagaagcccg cgggcaagtt cgacgacatg gccgacgaca
     6421 tcccgttcta tccgccccga tggcgctata attcgcgccg attcaggaac ttgccggacg
     6481 acgatgccga tctacgaata ccgctgtacc gactgcggct tccaggacga gtacctgcag
     6541 agggtctcgg agccgccgtt gaccgattgc ccctcgtgcg ggaagcccgc gtttcgcaag
     6601 ctggtgagcg cggcggggtt ccagctgaag ggcagcggct ggtacgcgac cgatttcaag
     6661 ggcgccggca aggccgcggc caaggccgac gcgcaaggcg cggcgaagcc ggaatccaag
     6721 accgaagcca agtccgaggc gaaggcggag gcgaaagccg aagccaagag cgcgccgacg
     6781 aagacgccgg cggccgcgaa ctcggacagt tgagtcgcta gccgggctcg ccaccggccc
     6841 agcgcaagct gggctttctt ctttgcaggg gaagcgtgcg gaaatacttc attaccggac
     6901 tgctgatctg gatcccactg gtcatcacga tctgggtcct ggacctgatc gtgacgacgc
     6961 tcgaccagtc gctgcacctg ctgccgccgc aatggcgcac cgagagcttc ctcggtttcc
     7021 acgtcccggg cctgggcgtg atcctgacgt tggtgatcgt gttcgtcacc ggcgtgctcg
     7081 ccaccaactt cctcggcgcg cgcctggtga acctgtggaa cgaaatcctg caccgcatcc
     7141 cggtggtcaa ctcgatctac tcgagcgtca agcagatctc cgacacgctg ttctcctcga
     7201 gcggccaggc gttccgcaag gcgctgctcg tgcagtggcc gcgcgaaggc atgtggacca
     7261 tcgctttcct gaccggcagg cccggcggcg acgtcgtcag gcacctgccg ggtgactacg
     7321 tcagcgtcta cgtgccgacc acgccgaacc cgaccggcgg ctacttcgtc atgatggcgc
     7381 gcaaggacgt catcgagctc gacatgtcgg tggacgcggc gctgaaatac atcatctcga
     7441 tgggcgtggt cgcgcccgga ccgggccggt agccggagcg atgcgcacgc actactgcgc
     7501 cgagctctcc gccgcgctgg tcggcaagca agtgacgctt gccggctggg cgcaccgtcg
     7561 gcgcgatcat ggcggcgtca tcttcatcga tttgcgcgac cgcgagggcc tcgcgcaggt
     7621 cgtgtgcgac cccgatcgcg cgcaggcctt ccgcgccgcc gagggcgtgc gcagcgagtt
     7681 cgtcctgcaa gtgaccggcc gggtgcgcgc tcggcccgaa ggcaccgtca acccgaacct
     7741 tgcctcgggc gcggtggagg tgctcgccga gtcggtggag atcctcaatc catcgctgcc
     7801 gccgccgttt cagatcgacg aggacaacct gtcggagtcc gtgcggctc
//
LOCUS       DBNHKE010000214         4507 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_515916, whole genome shotgun sequence.
ACCESSION   DBNHKE010000214 DBNHKE010000000
VERSION     DBNHKE010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_515916"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="AC16O9_14570"
     CDS             <1..1803
                     /locus_tag="AC16O9_14570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6517875.1"
                     /translation="ALDWEHGDAAAVDAAFASAAHRIAVRLEDTRVAPSALEPRAASA
                     AWDGKRYTLTVSTQGVALVRKVLAENVFKCPPETIRVLTHDVGGGFGMKVQTYAEYAA
                     LMAAAKRVGRPVHWCATRLESFLADTHGRDGVLEGELALDRDGKFLALRARTQVGIGA
                     YASTYAAIVATNNTKNCLSSVYRIPAIHAGVQMVLTNAAPLGPYRGAGRPEAIYLVER
                     LIDEAARATGIDRVALRRRNLIPASAMPYKAPSGQTYDSGEFEAVLDAALELADWKGF
                     AARRKASEAKGRLRGIGLCCFLEVAGGAPLTEVADLRFEPDGTAVLRTGAQAMGQGQM
                     TTLPLILARQLQIDASRVRIVQGDSDEVPGGIPTVASRSTMMVGGAMGLAAEEAINKG
                     KGVAAELLEAAAADIEYGKGEFRVAGTDRRVGWRELAQGRADALKTVATFAAPEMSFP
                     NGCHVCEVEVDPETGVVSVAGYAAVDDVGHVVHQAIVEGQIHGGVAQGLGQVLGEHLV
                     YGEDGQLLTASFMDYAVPRADDLPMLALGHHVAPCTTNPLGAKGAGESGVAGSLPAGV
                     NAILDALASRGVRHLDMPMTPQRVWRALQKGGKA"
     gene            1800..2567
                     /locus_tag="AC16O9_14575"
     CDS             1800..2567
                     /locus_tag="AC16O9_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6517876.1"
                     /translation="MNTSAKGLRVIVTAGAAGIGLAIARTFRDHGAKVHVCDVDEKAL
                     AALDKDIQQTKADVSNVADVERLFADAQRTLGGLDVLVNNAGIAGPTAKVEDIKVEDW
                     DRCIAVDLNGMFYCTRRGMPLIKAAGGGSIINLSSTAGRLGFALRTPYAAAKWAVVGF
                     TQSLAAEAGPDGVRVNCIQPGVVEGERVERVIDAKARGLGITNDDMRARLLESVSLRS
                     MVSAQDVANTALFLATDAGQHISGQAISVCGGVRYMV"
     gene            2578..3513
                     /gene="kce"
                     /locus_tag="AC16O9_14580"
     CDS             2578..3513
                     /gene="kce"
                     /locus_tag="AC16O9_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,5-dioxohexanoate:acetyl-CoA acetone
                     transferase"
                     /protein_id="HIR6517877.1"
                     /translation="MAAAQGKVIITCAVTGSIHTPSMSPHLPVTPAEIAKAAIGAAQA
                     GAAIVHLHARNPKDGSPSQDPALFHEFLPQIAVASDVVINLTTGGAATMTIEERLQPA
                     LQLKPEVASLNMGSMNFGLYEMLGRFKEFKHDWEKPYLADSDERIFKNTFRDIAYILT
                     SCSENGTRFEIECYDIGHLYTAAHFLDRGLIKPPMLIQSVFGIRGGIGGHPEDVLHMK
                     RTADRLFGDAYYWSVLGAGRNQMAIAAQAAVMGGNVRVGLEDSLWLGRGQLAKSNAEQ
                     VAKARRIIEEVGLQVATPNEAREMLKLKGAKNVDF"
     gene            3515..4315
                     /locus_tag="AC16O9_14585"
     CDS             3515..4315
                     /locus_tag="AC16O9_14585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6517878.1"
                     /translation="MPRPPPLACDCHIHVFGPFARYPLDAARKYTPDEALLEDYIKVA
                     TALGTGRVVLVQPSVYGVDNSCQRAAIRSLEAHARGIAVIDDSVSDAQLGELNGAGFV
                     GARLNFMRAGELESLERTAARVKDLGWHVQLHLPGKMIPQIAERLLKLPTDFVIDHFG
                     RMDAWQGCVQPAFRELLRLVDSGRCWVKLSAPYRMDGAPYPRVAPFARELVKRNPERM
                     LWGTDWPHPDVAVVPEDAGLLRTMFDWVPDAETRQRILVDNPAKLYRF"
     gene            4315..>4507
                     /locus_tag="AC16O9_14590"
     CDS             4315..>4507
                     /locus_tag="AC16O9_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517879.1"
                     /translation="MKWHIHWPAMVLALVGLVFFAADRLTRTPAVVHVVPGQVIEIPV
                     ARDGHYYVEGSVNGTPLKFM"
BASE COUNT          711 a         1534 c         1622 g          640 t
ORIGIN      
        1 gcgctcgact gggagcacgg cgacgcggcg gcggtggacg ccgcattcgc aagcgccgcg
       61 cacagaatcg ccgtgcgcct cgaggacacg cgcgtcgcgc cgagcgcgct cgagccgcgc
      121 gcggcgagcg ccgcctggga cggcaagcgc tacacgctca cggtctccac gcagggcgtg
      181 gcgctggtgc gcaaggtcct cgccgagaac gtgttcaagt gcccgccgga gacgatccgc
      241 gtgctgacgc acgacgtcgg cggcggcttc ggcatgaagg tgcagaccta cgccgagtac
      301 gcggcgctca tggccgcggc gaagcgggtc gggcggcccg tgcactggtg cgcgacgcgc
      361 ctggagagct tcctcgccga cacgcacggg cgcgacggcg ttctcgaggg cgagctggcg
      421 ctggaccggg acgggaagtt cctcgccctg cgcgcgcgca cgcaggtcgg catcggggct
      481 tacgcctcga cctacgccgc gatcgtcgcc accaacaaca ccaagaactg cctctcgagc
      541 gtgtaccgga tcccggcgat ccacgccggc gtgcagatgg tgctgaccaa cgccgcgccg
      601 ctcgggccgt atcgcggcgc cgggcgtccg gaggcgatct acctggtcga gcggctgatc
      661 gacgaggcgg cgcgcgccac cggcatcgat cgcgtcgcgc tgcggcgccg gaacctgatc
      721 ccggcctccg ccatgccgta caaggcgccg agcggccaga cgtacgacag cggcgagttc
      781 gaggcggtgc tcgacgcggc cctcgagctt gccgactgga agggcttcgc cgcgcgccgc
      841 aaggcgtccg aggcgaaggg tcgcctgcgc ggcatcgggc tgtgctgctt cctggaggtc
      901 gcggggggtg cgccgctcac cgaggtcgcc gacctgcgct tcgagcccga cggcaccgcc
      961 gtgctgcgca ccggcgcgca ggccatgggc cagggccaga tgaccaccct gcccctgatc
     1021 ctcgcgcggc agctgcagat cgacgcgtcg cgcgtgcgga tcgtgcaggg cgacagcgac
     1081 gaagtgccgg gcgggattcc gacggtcgct tcgcgctcca ccatgatggt cggcggcgcc
     1141 atggggcttg ccgccgaaga ggcgatcaac aagggcaagg gcgtcgccgc cgagctgctc
     1201 gaggccgccg ccgcggacat cgagtacggc aagggcgagt ttcgcgtcgc cggcaccgac
     1261 cggcgcgtgg gctggaggga gctcgcgcag ggcagggccg acgcgctcaa gacggtggct
     1321 actttcgccg cgccggagat gagctttccc aacggctgcc acgtgtgcga agtggaggtc
     1381 gatccggaaa ccggcgtggt gagcgtggcg ggctatgctg cggtggacga cgtcgggcat
     1441 gtcgtgcacc aggcgatcgt cgaaggccag atccacggcg gcgttgcgca ggggctcggc
     1501 caggtgctgg gcgagcacct ggtgtacggc gaggacgggc agctgcttac cgcgtcgttc
     1561 atggactacg ccgtgccgcg tgccgacgac ctgccgatgc tcgcgctcgg ccaccacgtt
     1621 gcgccctgca ccaccaaccc gctcggcgcc aagggcgcag gcgaatccgg cgtcgccggg
     1681 tcgctgcccg cgggcgtcaa tgcgatcctc gacgcgctcg ccagccgcgg cgtacgtcat
     1741 ctcgacatgc cgatgacgcc gcagcgcgtc tggcgggctt tgcagaaagg ggggaaggca
     1801 tgaacacatc ggccaagggc ctgcgcgtca tcgtcaccgc gggcgccgcg ggcatcggcc
     1861 tcgccatcgc gcgcaccttt cgcgaccacg gcgccaaggt gcacgtgtgc gacgtcgacg
     1921 agaaagcgct cgccgcgctc gacaaggaca tccagcagac gaaggccgac gtctccaatg
     1981 tcgccgatgt agagcggctc ttcgccgacg cgcagcgcac gctcggcggg ctcgacgtgc
     2041 tggtgaacaa cgccggcatc gccggcccta ccgccaaggt cgaggacatc aaggtcgagg
     2101 actgggaccg ctgcatcgcc gtcgacctga acggcatgtt ctactgcacg cgtcgcggca
     2161 tgccgctcat caaggcggcg ggcggcggct cgatcatcaa cctgtcctcg accgcggggc
     2221 gcctgggctt cgcgctgcgc acgccctacg cggcggcgaa gtgggcggtg gtcggcttca
     2281 cccagagtct cgccgccgag gcggggcccg acggggtgcg cgtcaactgc atccagccgg
     2341 gcgtggtgga aggcgagcgc gtcgagcgcg tgatcgacgc caaggcgcgc gggctgggca
     2401 ttaccaacga cgacatgcgc gcgcggcttc tcgagagcgt gagcctgagg agcatggtca
     2461 gcgcccagga cgtggccaac acggcgctgt tcctcgccac cgacgcgggc cagcacattt
     2521 ccgggcaggc catctcggtc tgcggcggcg tacgctacat ggtctaggag aaatacgatg
     2581 gcagcagccc agggcaaggt catcatcacc tgcgcggtga cggggtcgat ccacacgccg
     2641 agcatgtcgc cgcacctgcc ggtgaccccg gccgagatcg ccaaggcggc gatcggcgcg
     2701 gcgcaggccg gcgccgcgat cgtgcacctg cacgcgagaa atcccaagga cggcagcccg
     2761 tcgcaggacc cggcgctgtt ccacgagttc ctgccgcaga tcgcggtggc gagcgacgtg
     2821 gtgatcaacc tcaccaccgg cggcgcggcg acgatgacca tcgaggagcg cctgcagccc
     2881 gcgctgcagc tcaagcccga ggtcgcctcg ctcaacatgg gctcgatgaa cttcggcctg
     2941 tacgagatgc tcgggcgatt caaggagttc aagcacgact gggaaaagcc ctacctcgcc
     3001 gattccgacg agcgcatctt caagaacacc ttccgggata tcgcctacat cctgacctcg
     3061 tgcagcgaga acggcacgcg cttcgagatc gagtgctacg acatcgggca cctctatacc
     3121 gcggcgcact tcctggaccg cgggctgatc aagccgccga tgctgatcca gtccgtgttc
     3181 ggcatccgcg gcggcatcgg cgggcatccc gaggacgtcc tgcacatgaa gcgcacggcc
     3241 gatcgcctct tcggcgacgc ctattactgg tcggtgctgg gcgccggccg caaccagatg
     3301 gcgatcgcgg cccaggctgc cgtgatgggc ggcaacgtgc gtgtcgggct ggaggacagc
     3361 ctgtggctcg ggcgcgggca gctggcgaag tccaacgccg agcaggtggc caaggcgcgg
     3421 cgcatcatcg aggaagtcgg gctgcaggtg gcgacaccca acgaggcccg cgagatgctg
     3481 aagctgaagg gtgcgaagaa cgtagacttc taggatgccc cggccgccgc cgctcgcctg
     3541 cgactgccac atccacgtct tcgggccgtt cgcgcgctac ccgctcgatg cggcgcgcaa
     3601 gtacacgccc gacgaggcgc tgctcgagga ctacatcaag gtggcgaccg cgctcggcac
     3661 cgggcgcgtg gtgctggtgc agccgagcgt ctacggcgtg gacaattcct gccagcgcgc
     3721 ggcgatccgc tcgctcgagg cgcacgcgcg cggcatcgcg gtgatcgacg actcggtgag
     3781 cgatgcgcag ctcggcgagc tgaacggcgc gggcttcgtc ggcgcgcgct tgaacttcat
     3841 gcgcgcgggc gagctcgagt cgctcgagcg caccgcggcg cgcgtgaagg atctcggctg
     3901 gcacgtgcag ctgcaccttc cgggcaagat gattccccag atcgccgagc ggctgctgaa
     3961 gctccccacc gacttcgtca tcgatcactt cgggcgcatg gatgcctggc agggctgcgt
     4021 gcagcccgcg ttccgggaat tgctcaggct cgtcgattcc ggaagatgct gggtaaagct
     4081 gtccgcgccc tatcggatgg acggcgcgcc ctatcccagg gtcgcgccgt tcgcccgcga
     4141 gctggtgaag cgcaatcccg agcgcatgct gtggggcacc gactggccgc atccggacgt
     4201 ggcggtggtg cccgaggacg cgggactgct gcggacgatg ttcgactggg tgccggacgc
     4261 ggagacgcgc cagcgcatcc tggtggacaa cccggcgaag ctgtaccgat tctgatgaag
     4321 tggcacatcc actggccggc gatggtcctg gccctggtcg gcctggtgtt cttcgccgcg
     4381 gaccgcctga cgcgcacgcc cgcggtggtg cacgtggtcc ccgggcaggt gatcgagatt
     4441 cccgtggcgc gggacgggca ctactatgtc gagggctccg tcaacggcac acccctgaag
     4501 ttcatga
//
LOCUS       DBNHKE010000215        16179 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_780382, whole genome shotgun sequence.
ACCESSION   DBNHKE010000215 DBNHKE010000000
VERSION     DBNHKE010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_780382"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="AC16O9_14595"
     CDS             <1..1492
                     /locus_tag="AC16O9_14595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6517880.1"
                     /translation="GEAYTDERELRRKDGSQLWCKVVGRAIDPAQPQEGSIWIYDDVS
                     AEHAAREGLERMVAARTSELRDAQERAQHLADHDALTGLPNRRILEDRLTQALALSQR
                     NRKQTAVMFVDLDRFKAINDSLGHAVGDQVLKEVARRLVKQLRVGDTVCRIGGDEFVV
                     VLPESKRSTDAAGVAAKIIETLSQPAQVADRELTVTPSIGIAVFPEDGRDAAMLIRNA
                     DAAMYHAKESGRANYQFFTEQMNQAAARRVALEGDLRRAEQRGELALHYEPIAAVKDR
                     TVVAHEARVRWQHARTGLVPAADLAVLAEETGLILGIGAWALREACRWCTFIGVEHEV
                     QVAVNLSARQFNDPGLIELVRRTLAESGLPPRLLSLEIGEAALMQQPDVAASTLRKLK
                     ELGVALAVDDFGAGSSSLAALRRFPLDRLKLDRSLVADAAGDPGAAALVGGIVGLAHA
                     LKLTVVAEGADTPAQRELLAALGCDYMQGLVAGAPVDADAAAEDYV"
     gene            1507..2298
                     /locus_tag="AC16O9_14600"
     CDS             1507..2298
                     /locus_tag="AC16O9_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517881.1"
                     /translation="MAPLVLHFSHANGFPAPCYEAMLAPLRRRYRVGYIEAIGTDPRY
                     PPTEGWPHLVEQLIDTLEREYGGEPVFGVGHSLGAYLSFLAAVRRPELFRAIVLLDAP
                     IIGAFKGRALGVVKRLGMVDRVTPAGMTRERRSVWTTREEATAHFRSKKLFRNFTDEC
                     LEHYVRYGLAGAPGRYRLLIDPEIEYRIYRTIPHDMGRHLRALKLPAGFVGGEQSDVL
                     RRVGLGAMRGPRFAKMGVPGGHLFPFEHPLVAAAALEELLGRLAP"
     gene            complement(2295..2945)
                     /locus_tag="AC16O9_14605"
     CDS             complement(2295..2945)
                     /locus_tag="AC16O9_14605"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6517882.1"
                     /translation="MIRTLLTSATLALTLAAPAAFPAPPATRDEALAALEHPVTERRA
                     EAVVWMANHGRMEDTGLLMKRLRDESAFVRGYAEQGLWLLWSRSGDAEIDRVMGRGVE
                     DMQSGRYEEAVAAFSDVIRRRPDFAEGWNKRATALFLAGAYRRSLADCDEVLKRNPQH
                     FGALSGYGQIYFQLGEYARAIEWWRRALDVNPNMLGVEIGIKRAEELLREKRGRST"
     gene            2962..3396
                     /locus_tag="AC16O9_14610"
     CDS             2962..3396
                     /locus_tag="AC16O9_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6517883.1"
                     /translation="MIALLLAPLELWAADDATAAPDPVLQQAREALSRQDYARSAAVL
                     REALVKAPANPDYHNLYAYSLRKAPGPDMDLVFKHYHEALRLAPKHRGAHEYVGEAYL
                     MVGNVGKAREHLAQLDRLCFFGCEEYSSLKQAIREYEAKGGR"
     gene            3482..4534
                     /locus_tag="AC16O9_14615"
     CDS             3482..4534
                     /locus_tag="AC16O9_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6517884.1"
                     /translation="MKPLRVAVAGAGYFCQFQLDGWKRIPGVELAGICNRDLAKAEAL
                     AARFGVARVFGALAPMLEATAPDLVDIVTPPVTHRDYVGEAARRRVATICQKPFGRTH
                     AEAQALVEVAKRAGILLAVHENFRFMPHFREMKRLLDAGRLGALHSIAFRLRPGDGQG
                     PEAYLARQGYFQKMPRFLVFETAIHLIDTFRFLAGEVAAVTARLRRLNPAIAGEDAGY
                     IIFEFANGATGLFDGNRLNDHVAANARRTMGEAWLEGSAGVLRLDGEARLWWKPHQQP
                     ETEHRYDRGRDDTFGGGACDALQAHVVRHLREGAPLENSGADYLANMRIMEAVYASHA
                     EGRRIEIDAFAPEELR"
     gene            4545..5759
                     /gene="otnK"
                     /locus_tag="AC16O9_14620"
     CDS             4545..5759
                     /gene="otnK"
                     /locus_tag="AC16O9_14620"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HIR6517885.1"
                     /translation="MLLGCIADDLTGATDLGVTLAREGLSVVQVNGVPDAKLEAPDAD
                     AVVVALKSRTNPPEEAVEWSLRALAWLRGAMASHIYFKVCSTFDSTPRGNIGPVADAL
                     LAALGREFALATPAYPRNQRTVYLGNLFVGAVPLAESGMRNHPLTPMTDSNLVRVMGA
                     QTKGRVGLVDFAAVEAGAAAVQARCEALRAEGCRYAIVDAIRDEHLVAAGEACAGMKL
                     SIGGAGLAMGIARALAKGAKKATGAALPKVDGAAAILSGSCSEATLAQVEAARAKFPS
                     LHLDPARLDVEAALAWAKPKLGAGPVLIYTSAPPSARAEGRADAIEQAFRALAKGLAA
                     AGVRRFVVAGGETSGAVAEALGVKALAIGPEIAPGVPWTAALGGKPMLLALKSGNFGG
                     PDFFTRAFEVLA"
     gene            5756..6388
                     /gene="otnC"
                     /locus_tag="AC16O9_14625"
     CDS             5756..6388
                     /gene="otnC"
                     /locus_tag="AC16O9_14625"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HIR6517886.1"
                     /translation="MTENQLREQLVMFGQSLYARGYAHGSSGNLSVRLPDGLLVTPTN
                     SCLGRLDPAKIAKLDKAGKHVSGDKPSKEGFLHLAMYEERASAQGIVHLHCTHCVALS
                     CLRHADAANVLPPLTAYYVMRIGKLPLVPYYRPGDRALAQAVREKARKHHAVLLANHG
                     PVVAGKSLEDAVYNAEEMEETAKLHLLLTGRDARPLEAAQIADLEAHFPS"
     gene            6401..7684
                     /locus_tag="AC16O9_14630"
     CDS             6401..7684
                     /locus_tag="AC16O9_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6517887.1"
                     /translation="MRPSFIFILADDLGYADLGCYGARSGCSPVLDRMAAEGLRFTEG
                     YANSSVCSPTRFALATGRWQHRLRGGADEPISGRKRGDPVLGLPPAHPTLASLLGDAG
                     YATALVGKWHLGYPPNFHPLRSGYQEFFGSLSGGFDYFTHCDTAGVHDLWEGEKEIHR
                     QGYMTDLLSERAVRYVKNARAPFLLSLHYSAPHWPWETRADEAEAKRIEKIWHTDGGS
                     LAVYQKMIHHMDEGIGQVLAALPDPRNTLVVFTSDNGGERFSDAWPLVGKKMDLLEGG
                     IRVPYIVRWPARVKAGGVTAQHAITMDWVATFLEAAGVKAHPDYPLDGLSLLKVLAEP
                     GAPFARDLFWRMKYRAQRAVRSGDWKYFASEDGEFLFNLAVDARERANYAKREPARLA
                     ELRAKYEAWEKSIPPIPPDAAFSIPYTRAEVAQPG"
     gene            complement(7681..7890)
                     /locus_tag="AC16O9_14635"
     CDS             complement(7681..7890)
                     /locus_tag="AC16O9_14635"
                     /inference="COORDINATES: protein motif:HMM:NF020908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HIR6517888.1"
                     /translation="MLALEWIAERKIEEAVSRGELDRLPNAGRPLDLYEDPLVPPEMR
                     MAKRILGNAEVSLEEIFTELRNRRR"
     gene            7911..9641
                     /locus_tag="AC16O9_14640"
     CDS             7911..9641
                     /locus_tag="AC16O9_14640"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6517889.1"
                     /translation="MSRRAYRILPTEARTFHPRLMGTRGAVASNSNLSASAGADVLKA
                     GGNAIDAAVAASFVEGVVNAQMHTIGGECPLLVRLAGESRVIAVNGNMAAPAKATPDA
                     FRARGFTDIPDAGILAAGVPAAFGALVTALREWGTLPLAEVLAPAIELAQNGFPVSEG
                     LRNQHKYGIAPMQARFEAEWPGSARLYLPVPEVGALFRNPALARSFEYLAAAKDPLQA
                     FYQGEIAAEIAKFSKAHDGLLGRADLAAFETRIEAPVSLRVGDTELYKTGYWSQGPAE
                     LQTLALMQQFDLKKIGFGTPDYCHLLVEAMKLAYADREQYYGDPAMAKVPEQTLLSPG
                     YAKKRAALIDMQRASLELRPGGAWNNAALLPEAEKLTPKDWGPGTVHVDAVDAKGNMA
                     SFTPSGAWISSAEVIEPLGFPLSTRMMTFYLEPAHHPNVVAPGKRPRTTLTPSMAFRN
                     GKPWMAFGTMGGDNQGQWLLQFYLCRAVFGMSIPEAIEAPRLSSEHTPGFFAPHASEP
                     NRVRIEPRFGAQVLDELRRRGHDLDVAPDWTEGFVSCAAFDEQSGLIEAGCDPRGSKA
                     ECFPAFAMAW"
     gene            9709..10671
                     /locus_tag="AC16O9_14645"
     CDS             9709..10671
                     /locus_tag="AC16O9_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HIR6517890.1"
                     /translation="MMTVLGSGEHRYRVVENWAKLPAGWEFKDVAAVACDRRDQVYVF
                     NRGEHPMMVFDRDGNFLRSWGEGSYPRAHGLHIDVDDTLYLTDDGGHFVRKCTPEGRV
                     LLELGVPGKPAPFMSGKPFHRCTHTALSPEGEIYVSDGYGNAKVHKYSPDGKLLLSWG
                     EPGSDPGQFNVVHNIVTDAEGWVYVADRENHRVQVFDARGRYETQWNNMHRPCGLYCC
                     RGKQLQFIIGELGPGMNINRDHPNLGPRLSIVDPEGRLVARLGGEQGPGLETGKFIAP
                     HGLAVDSRGDIYVGEVSYTEFPRLYPERGIPKGLRSLQKLTKVA"
     gene            10686..10994
                     /locus_tag="AC16O9_14650"
     CDS             10686..10994
                     /locus_tag="AC16O9_14650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6517891.1"
                     /translation="MIHVIAVITAKPGKREEILAHFRANVPAVRAEKGCIEYGPAVDA
                     DPAPQMQTKYGPDTFVAIEKWESMDALKAHAAAPHMAAYAAKVKDMIAGRVIHILTPA
                     "
     gene            complement(10981..11901)
                     /locus_tag="AC16O9_14655"
     CDS             complement(10981..11901)
                     /locus_tag="AC16O9_14655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6517892.1"
                     /translation="MEVAVLGAGAVGCYYGGMLARAGHAVTLIGRPVHVAAFAARGLR
                     FEGLKFDEQVAVGASTEASAVRGCRVVLFCVKSTDTESAAEQMAPHLDAGALVVNLQN
                     GVDNTERIAARIAQPVIPAVVYVATEMAGPGHLKHHGRGDLVIGELNAKLPAGALAQV
                     KRWFEAAGVPVAISGNVAGELWAKLVVNCAYNALSAISQLPYGKMIEGPGIRDVMREV
                     VEETLAVARASGVTMAPDILERTYRIAEAMPAQYSSTAQDLARGKPTEIEHLNGFVAR
                     RGASLGVPTPANRTLAALVRLLESKPLKPA"
     gene            11943..12710
                     /locus_tag="AC16O9_14660"
     CDS             11943..12710
                     /locus_tag="AC16O9_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR6517893.1"
                     /translation="MTIHSLTARRAADPVREAKIHLAAAHRLAVLHELEEGIDNHFTV
                     TVPGATDRYLILPFGLHWSEARASDMIVFDESGRTLEGEGVVELSAQCIHAPIHRLTG
                     ARVVLHTHQTWALALNMLKENRLLPASQTAAYFHGHVAYDDTYAGTADFVEEGERLAR
                     LIGDKHVLFMKNHGVLVVGDTVAQAYRRIYKLERVCRAQVLAMGTGKPLEVLSDEIAA
                     QVQSPPVDERHSRAERERLYFEAMMRVLDRENPGYAD"
     gene            12755..13957
                     /locus_tag="AC16O9_14665"
     CDS             12755..13957
                     /locus_tag="AC16O9_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517894.1"
                     /translation="MPFIMAATLIDMMAIGIIIPVLPAMVGEFTGSQVHQAYWYGVVT
                     FAFSLANFFGSPFLGALSDSRGRRTVLLLGFCGMAFSFLATVLATALWVLVAVRVVSG
                     AMQANISVANAYVADITPPEMRAKRFGMLGAMFGIGFILGPVLGGLLGAIDLRLPFFV
                     AGTLALCNLAYGYFVLPESLPPERRRAFEWRSLNPVGALRGLAQTRGVGVLVAVIACS
                     GLAQFVLYTTWVLYTTFKFGWGPLENGWSLAAVGLVSAIVQGLLLGRLLRIFSPRRLA
                     VLGLLSSTVAFVLWGAATEGWMMFAIIFVNLLGVTVAASIQSLISQAVDARSQGQALG
                     AAGGLNSLMAVVAPAIGAPLLALVSHLPQGDWRIGTPFYFCAALQAAALALAVIHFRG
                     ERRARLAG"
     gene            complement(13954..15018)
                     /locus_tag="AC16O9_14670"
     CDS             complement(13954..15018)
                     /locus_tag="AC16O9_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6517895.1"
                     /translation="MVERNNGLRTHVLEAGDPRAPGLLLLHGFPELAFSWRKVMLPLA
                     EAGYHVMAPDQRGYGRTTGWDPDYDGDLASYHLLNLARDALGVVRALGHDTVHLAGHD
                     FGSWVAAWCALVRPDVFRAVALMSAPFTGPPGLVPEAPDPIHEALAALPRPRKHYQAY
                     YRTREANRDMTHCAQGLHAFLRAYFHMKSADWTQNRPAPLAGWTAAELARMPTYYVMD
                     LHETMAQTVAHEMPSREQIAACRWLTEAELAVYSAEFGRTGLQGGLQWYRARVEPPLP
                     RLFELFAGRRIEVPACFIAGKSDWGPYQRPGNLEDMRDKACARYLGTHLVDGAGHWVQ
                     QEQPEAVAGLLREFYGARRG"
     gene            complement(15060..16079)
                     /locus_tag="AC16O9_14675"
     CDS             complement(15060..16079)
                     /locus_tag="AC16O9_14675"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR6517896.1"
                     /translation="MLETTVAGSLPKPFWLAETDKLWPAWKASGAELEKAKRDATLLW
                     LKEQEDAGIDIVSDGEQSRQHFVHGFLEFVEGIDFEHKVKMGIRDNRYEAMVPVVTGP
                     IRLKGRVHQAEARLARAHTKKKLKFTMPGPMTIVDTIADRHYGDKVKLAMAFAELLNQ
                     EARALEKDGVDVIQFDEPAFNVYMDDVNRWGIKALERAAKGLRCTTCVHICYGYGIKA
                     NLEWKEKLGGEWRQYEKIFPALARSKIKQVSLECIHSHVPPELMKLLKGKDVLVGVID
                     VASDKVETPQEVAATIRNALKYVPRKHLFPCTNCGMAPMRRDIALAKLKALGAGAALA
                     RKRAR"
BASE COUNT         2362 a         5666 c         5675 g         2476 t
ORIGIN      
        1 gggcgaggcc tatacggacg agcgcgagct gcgccgcaag gacggcagcc agctctggtg
       61 caaggtcgtc ggccgcgcca tcgacccggc gcaaccgcag gagggctcga tctggatcta
      121 cgacgacgtt tcggccgagc acgccgcgcg cgaaggcctc gagcgcatgg tggccgcgcg
      181 caccagtgag ctgcgcgatg cgcaggagcg cgcccagcac cttgccgacc acgacgcgct
      241 caccggcctg ccgaatcgcc gcatcctcga ggaccgcctg acgcaggcgc tcgcgctcag
      301 ccagcgcaac cgcaagcaga ccgcggtgat gttcgtcgat ctcgatcgct tcaaggcgat
      361 caacgattcg ctcggccacg cggtgggcga ccaggtgctg aaggaagtgg ccaggcgcct
      421 ggtgaagcag ctgcgcgtcg gcgacaccgt gtgccgcatc ggcggcgacg aattcgtggt
      481 ggtgctgccc gagtcgaagc gctcgaccga cgccgccggc gtggccgcca agatcatcga
      541 gacgctgtca cagccggcgc aggtcgccga ccgcgagctc acggtcaccc cctcgatcgg
      601 catcgccgtg ttcccggagg acgggcgcga cgccgccatg ctgatccgca acgccgacgc
      661 cgcgatgtac cacgccaagg agtccgggcg cgccaactac cagttcttca ccgagcagat
      721 gaaccaggcg gcggcgcgcc gcgtggcgct ggagggcgac ttgcgccgcg ccgagcagcg
      781 cggcgagctg gcgttgcact acgagccgat cgccgcggtg aaggaccgca cggtggtggc
      841 gcacgaggcg cgcgtgcgct ggcagcacgc gcgcaccggg ctggtgcctg ccgccgacct
      901 cgccgtgctg gcggaagaaa cggggctgat cctgggcatc ggcgcctggg cgctgcgcga
      961 ggcctgccgc tggtgcacgt tcatcggcgt ggagcacgag gtgcaggtcg cggtgaacct
     1021 gtcggcgcgc cagttcaatg acccgggcct gatcgagctg gtgcggcgta cgctcgccga
     1081 gagcgggctg ccgccgcgcc tgctgtcgct cgagatcggc gaggcggcgc tgatgcagca
     1141 gcccgacgtc gcggcgagca cgctgcgaaa gctcaaggag ctgggggtgg cgctggcggt
     1201 cgacgacttc ggtgcgggca gttcgagcct cgccgcgctg cgccgctttc ccctggatcg
     1261 gctcaagctc gaccggagcc tggtcgccga cgcggcgggc gacccgggcg ccgccgcgct
     1321 cgtcggcggc atcgtcgggc tcgcccacgc gctcaagctc acagtggtgg ccgaaggcgc
     1381 ggacacgccg gcgcagcgcg aactgctcgc cgcgctcggc tgcgattaca tgcaggggct
     1441 ggtcgccggc gcgccggtgg acgccgacgc ggccgccgag gactacgtct agtcgattgc
     1501 ggccgagtgg cgccgctcgt tctgcatttt tcgcatgcca acggctttcc cgcgccgtgc
     1561 tacgaagcca tgctcgcacc gctgcgccgg cgctatcgcg tcggctacat cgaggcgatc
     1621 ggcacggacc cgcgctaccc gccgacggag ggctggccgc atctcgtcga gcagctgatc
     1681 gatacgctgg agcgcgagta cggcggcgag cccgtcttcg gcgtcggcca ttcgctcggc
     1741 gcctacctca gctttctcgc cgccgtgcgc cgccccgagc tgtttcgcgc catcgtgctc
     1801 ctcgatgcgc ccatcatcgg cgccttcaag ggccgcgcgc tcggggtggt gaagcgcctc
     1861 ggcatggtcg accgcgtcac gcccgccggc atgacgcgcg agcggcgcag cgtctggacc
     1921 acgcgcgagg aggcaaccgc gcacttccgc tcgaagaagc tgttccgcaa cttcaccgac
     1981 gagtgcctcg agcactacgt gcgctacggc ctcgccggcg cgccggggcg ctaccggctt
     2041 ctcatcgacc cggagatcga gtaccggatc taccgcacca tcccgcacga catgggccgg
     2101 cacctgcgag cgctcaagct gccggcgggc ttcgtcggcg gcgagcaatc cgacgtgctg
     2161 cggcgcgtgg ggctcggggc gatgcgcggc ccgcgctttg ccaagatggg cgtgcccggc
     2221 gggcacctgt tcccgttcga gcacccgctc gtcgccgccg ccgcgctcga ggagctgctc
     2281 ggccggctcg cgccctaggt gctgcgcccg cgcttttcgc gcagcagctc ctcggcgcgc
     2341 ttgatgccga tctcgacgcc gagcatgttg gggttcacgt ccagcgcgcg gcgccaccac
     2401 tcgatcgccc tggcgtactc gccgagctgg aagtagatct ggccgtagcc cgagagcgcg
     2461 ccgaagtgct gcgggttgcg tttcagcacc tcgtcgcaat cggcgagcga gcggcggtac
     2521 gcgccggcga gaaacagcgc cgtggcgcgc ttgttccagc cttcggcgaa gtcgggccgc
     2581 cggcggatca cgtcgctgaa tgcggctacc gcctcctcgt accggcccga ctgcatgtcc
     2641 tcgacgccgc gccccatgac gcggtcgatc tcggcgtcgc ccgagcggct ccacagcagc
     2701 cacaatccct gctcggcgta gccgcgcacg aaggcgctct cgtcgcgcag gcgcttcatc
     2761 agcaggccgg tgtcttccat gcgcccgtgg ttggccatcc agaccaccgc ctcggcgcgg
     2821 cgctcggtga ccgggtgctc gagcgcggcg agcgcttcgt cgcgcgttgc gggaggcgcc
     2881 gggaatgcgg ccggtgccgc cagtgttaac gccagggtcg ccgaggtaag gagggtccgg
     2941 atcatgatgc ttcgtgctct gatgatagcg ctgctcctcg ccccgctgga actgtgggcc
     3001 gcggacgacg caactgcggc cccggatccc gtgctgcaac aggcgcgcga ggccctctcg
     3061 cgccaggact acgccaggtc ggccgccgtg cttcgcgagg cgctcgttaa ggcgccggcc
     3121 aaccccgact accataacct gtacgcctac agcctgcgca aggcgcccgg tccggacatg
     3181 gacctcgtgt tcaagcacta tcacgaggcg ctgcgcctcg cgcccaagca ccgcggcgcg
     3241 cacgagtacg tcggcgaggc ctacctgatg gtgggcaacg tgggaaaggc gcgcgagcac
     3301 ctggcgcagc tcgaccgcct gtgcttcttc ggctgcgagg agtacagcag cctgaagcag
     3361 gcgatccgcg agtacgaagc aaagggcggc cgctagggcc gcggctggtg tagatttcgc
     3421 cagcgatggc tggcgcgccg gatccgttcg acattgcaat gcttcccggc gacctgtggg
     3481 catgaagccg ctgcgcgtgg cggtggcggg tgccgggtat ttctgccagt tccagctcga
     3541 cggctggaag cgcattcccg gggtggagct cgcaggcatc tgcaatcgcg acctcgccaa
     3601 ggccgaggcg cttgcggcgc gcttcggcgt cgcgcgcgtg ttcggcgcgc tcgcgccgat
     3661 gctggaggcg acggcaccgg acctcgtcga catcgtcacg ccgccggtca cgcaccgcga
     3721 ctacgtcggc gaggcggcgc gccggcgcgt tgcgacgatc tgccagaagc cgttcgggcg
     3781 cacccacgcc gaggcgcagg cgctcgtcga ggtcgcgaag cgcgccggca tcctgctcgc
     3841 ggtgcacgag aacttccgct tcatgccgca cttccgcgag atgaagcgcc tgctcgacgc
     3901 ggggcggttg ggcgcgctgc actcgatcgc ctttcgtctg cgcccgggcg acgggcaggg
     3961 gcccgaggcc tacctcgcgc gccagggcta cttccagaag atgccgcgct ttctggtgtt
     4021 cgagaccgcg atccacctca tcgacacctt ccgctttctc gcgggcgagg tggcggcggt
     4081 gacggcgcgg ctgcggcgcc tcaatccggc gatcgcgggc gaggacgccg gctacatcat
     4141 cttcgagttc gcgaacggcg ccacgggcct cttcgacggc aaccgcctga acgatcatgt
     4201 ggccgccaat gcgcgccgca ccatgggcga ggcctggctc gaaggctcgg cgggcgtgct
     4261 gcgcctggat ggcgaggcgc gcctgtggtg gaagccgcac cagcagcccg aaaccgagca
     4321 ccgctacgac cggggccgcg acgacacctt cggcggcggc gcctgcgacg cgctgcaggc
     4381 gcacgtggtg cgccacctgc gcgagggcgc gccgctggag aacagcggcg ccgactacct
     4441 cgccaacatg cgcatcatgg aggcggtgta cgcctcgcac gcggaaggac ggcgcatcga
     4501 aatcgatgct ttcgcgccgg aggaattgcg ctagattgcg cgtcatgctc ctcggctgca
     4561 tcgcggacga cctgaccggc gccaccgacc tcggcgtgac gctcgcgcgc gagggcctgt
     4621 cggtggtgca ggtgaacggc gtgcccgatg cgaagctcga agcgccggac gccgacgcgg
     4681 tggtggtggc gctgaagtcg cgcaccaatc ctcccgagga agcggtcgag tggtcgctgc
     4741 gcgcgctcgc ctggctgcgc ggcgcgatgg cgagccatat ctacttcaag gtgtgctcga
     4801 ccttcgattc gacgccgcgc ggcaacatcg gcccggtggc cgacgcgctg cttgcggcgc
     4861 tcggccggga gttcgccctg gcgacgcctg cctatccgcg caaccagcgc acggtgtacc
     4921 tgggcaacct gttcgtcggc gcggtgccgc tcgccgaatc gggcatgcgt aaccatccgc
     4981 tcacgccgat gaccgactcg aacctggtgc gcgtgatggg cgcgcagacc aaggggcgcg
     5041 tcgggctggt ggatttcgcg gcggtggaag cgggcgccgc ggcggtgcag gcgcgctgcg
     5101 aggcgctgcg cgccgagggc tgccgctatg cgatcgtcga cgcgattcgc gacgagcacc
     5161 tggtcgcggc cggggaagcc tgcgccggca tgaagctctc gatcggcggt gcgggtctgg
     5221 ccatgggcat cgcgcgggcg ctggcgaaag gcgcgaagaa agccacgggc gctgcgctgc
     5281 ccaaagtcga cggggcggcg gcgatcctct cgggcagctg ctcggaggcg acgctcgcgc
     5341 aggtggaagc cgcgcgcgcg aaattcccgt cgctgcacct cgatccggcg cggctcgacg
     5401 tcgaggcggc actcgcgtgg gcgaagccga agctcggcgc aggtccggtg ctgatctaca
     5461 cgtccgcgcc gccctcggcg cgcgccgagg ggcgggcgga tgcgatcgag caggcgttcc
     5521 gcgcgctggc aaagggcctc gccgccgcgg gcgtgcgccg cttcgtggtg gcgggcggcg
     5581 aaacctcggg cgcggtggcc gaggcactgg gcgtcaaggc gctcgccatc gggccggaga
     5641 tcgcgcccgg cgtgccctgg accgcggcgc tcggcggcaa gccgatgctg ctcgcgctga
     5701 agtcgggcaa cttcggcggc ccggacttct tcaccagggc gttcgaggtg ctcgcatgac
     5761 cgagaaccag ctgcgcgagc agctcgtcat gttcgggcag tcgctgtacg cgcgcggcta
     5821 cgcgcacggc agctccggca acctctccgt gcgcctgccc gacgggctgc tggtaacgcc
     5881 gaccaactcg tgcctggggc ggctcgaccc ggcgaagatc gcgaagctcg acaaggcggg
     5941 caagcacgtc tcgggcgaca agccctcgaa ggagggcttt ctccacctgg cgatgtacga
     6001 ggagcgcgcg agcgcccagg gcatcgtgca tctgcattgc acgcactgcg tcgccttgtc
     6061 gtgcctgcgg catgccgacg ctgcgaacgt gctgccgccg ctcaccgcgt actacgtgat
     6121 gcgcatcggc aagctgccgc tcgtgccgta ctaccggccg ggcgaccgcg cgctcgcgca
     6181 agcggtgcgc gagaaggcgc ggaagcatca cgcggtgctc ctcgccaacc acgggccggt
     6241 ggtggcggga aagtcgctcg aggacgcggt gtacaacgcc gaggagatgg aggagacggc
     6301 caagctccac ctcctgctca ccgggcgcga cgcgcggccg ctcgaggcgg cgcagatcgc
     6361 cgatctcgag gcgcactttc cgagctgacg cgcggcgaaa atgcggccca gcttcatctt
     6421 catcctcgcc gacgacctcg gctacgccga cctcggctgc tacggcgctc gctccggctg
     6481 ctcgccggtg ctcgatcgca tggcggccga gggcctgcgc ttcaccgagg gctacgcgaa
     6541 ttcgtcggta tgctcgccga cgcgcttcgc gctcgccacc gggcgctggc agcatcgact
     6601 gcggggcggc gccgacgagc cgatctcggg aagaaagcgc ggcgatccgg tgctcgggct
     6661 gccgcccgcg cacccgacgc tcgcctcgct cctcggcgat gcgggctacg ccacggcgct
     6721 cgtcggcaag tggcacctgg gctatccgcc gaacttccat ccgctgagaa gcggctacca
     6781 ggaattcttc ggctcgctct cgggcggctt cgactacttc acgcattgcg acaccgcggg
     6841 cgtgcacgac ctgtgggagg gcgagaagga gatccaccgc cagggctaca tgaccgacct
     6901 cctgtccgag cgcgccgtgc ggtacgtgaa gaacgccagg gcgcctttcc tcctgtcgct
     6961 gcactacagc gcgccgcatt ggccgtggga gacgcgcgcg gacgaggccg aggcgaagcg
     7021 catcgagaag atctggcata ccgacggcgg ctcgctcgcc gtgtaccaga agatgatcca
     7081 ccacatggac gagggcatcg gccaggtgct cgccgcgctg cccgatcccc ggaacacgct
     7141 cgtcgtcttc accagcgaca acggcggcga gcgcttctcc gacgcgtggc cgctggtcgg
     7201 caagaagatg gacctgctcg agggcggcat ccgcgtgcct tacatcgtgc gctggcccgc
     7261 gcgcgtgaag gcgggcggcg tcacggcgca gcacgcgatc accatggatt gggtggcgac
     7321 cttcctcgag gcggcgggcg tgaaggcgca tcccgactat ccgctcgacg ggctgagcct
     7381 gctcaaagtg ctggcagagc cgggcgcgcc gttcgcgcgc gacctcttct ggcgcatgaa
     7441 gtaccgggcg cagcgcgcgg tgcgctcggg cgactggaag tacttcgcga gcgaggacgg
     7501 cgagttcctg ttcaacctcg cggtcgatgc gcgcgagcgc gccaactacg ccaagcgcga
     7561 gccggcgcgc cttgccgagc tgcgcgccaa gtacgaagcc tgggagaagt cgattccgcc
     7621 gattccgccg gacgcggcgt tcagcattcc ctatacgcgc gccgaggtgg cgcaacccgg
     7681 ctagcgccgg cggttacgca gctcggtgaa gatctcctcg agcgacactt ccgcattgcc
     7741 gaggatgcgc tttgccatgc gcatctcggg cggcaccagc ggatcctcgt agaggtcgag
     7801 cggccggccg gcgttcggca gccggtcgag ctcgccgcgc gaaacggcct cctcgatttt
     7861 gcgttcggcg atccattcca gcgcgagcat tcggctattc tacggcgacg atgagccggc
     7921 gcgcctaccg catcctgccc accgaggcgc gcaccttcca cccgcggctc atgggcacgc
     7981 gcggcgccgt ggcttcgaac agcaaccttt cggccagcgc cggcgccgac gtgctgaagg
     8041 cgggcggcaa cgccatcgac gccgcggtag cggcgagttt cgtcgaaggc gtcgtcaacg
     8101 cgcagatgca tacgatcggc ggcgaatgcc cgctgctcgt gaggctcgcg ggcgaatcgc
     8161 gggtgatcgc ggtgaacggc aacatggcgg cgccggcgaa ggcgacgccc gacgcgtttc
     8221 gcgcgcgcgg tttcacggac atcccggacg caggcatcct tgccgcgggt gtgcccgccg
     8281 ccttcggtgc gctggtgacc gcgctgcgtg agtggggcac gctgccgctc gctgaggtgc
     8341 tcgcgccggc gatcgagctg gcgcagaacg gctttccggt gtccgagggc ctgcgcaacc
     8401 agcacaagta cggcatcgcg ccgatgcagg cgcgcttcga ggccgagtgg ccggggagcg
     8461 ccaggctcta cctgcccgtg cccgaggtgg gcgcgctgtt ccgcaacccg gcgctcgcgc
     8521 gcagcttcga gtacctcgcc gcggcgaagg atccgctgca ggctttctac cagggcgaga
     8581 tcgccgccga gatcgcgaag ttctcgaagg cgcacgacgg cctgctcggg cgcgcggacc
     8641 tcgccgcctt cgagacgcgc atcgaggcgc cggtgtcgct gcgcgtgggc gacaccgagc
     8701 tctacaagac cggctactgg tcgcagggac ccgccgagct gcaaacgctc gcgctgatgc
     8761 agcagttcga cctgaaaaag atcggcttcg gcacgcccga ctactgccac ctgctggtgg
     8821 aggcgatgaa gctcgcctac gccgaccgtg agcagtacta cggcgacccg gctatggcga
     8881 aggtgcccga gcagacgctg ctctcgcccg gctacgcgaa gaagcgcgcg gcgctcatcg
     8941 acatgcagcg cgcgagcctg gagctgcgcc cgggcggcgc gtggaacaac gccgcgctcc
     9001 tgcccgaggc cgagaagctc acgcccaagg actggggccc gggcacggtg cacgtcgatg
     9061 cggtggacgc caagggcaac atggcttcgt tcacgccgag cggcgcgtgg atctcgtcgg
     9121 cggaagtgat cgagccgctc ggctttccgc tttcgacgcg catgatgacc ttctacctgg
     9181 agcccgcaca ccacccgaac gtggtcgctc ccggcaagcg cccgcgcacc acgctgacgc
     9241 cgtcgatggc gttcaggaac ggcaagccct ggatggcgtt cggcaccatg ggcggcgaca
     9301 accaggggca gtggctgctg cagttctacc tgtgccgcgc ggtgttcggc atgtcgattc
     9361 ccgaggcgat cgaggcgccg cggctgtcct ccgagcacac accggggttc ttcgccccgc
     9421 atgcgagcga gccaaaccgc gtgcgcatcg agccgcgctt cggcgcccaa gtgctggacg
     9481 agctgcgccg gcgcggccat gatctggacg tggcgcccga ctggaccgaa gggttcgtct
     9541 cgtgcgccgc attcgacgag caaagcggcc tgatcgaagc cggctgcgac ccgcggggct
     9601 cgaaggccga atgctttccc gcgttcgcga tggcttggta aatggggaca gacctcattt
     9661 tttcgctctg cggaataatg aggtctgtcc ccatttaaag gacggtggat gatgacggta
     9721 ttgggcagcg gcgagcaccg ctaccgcgtg gtggagaact gggcgaagct gcccgcgggc
     9781 tgggagttca aggacgtggc ggcggtcgcc tgcgaccgca gggaccaggt ctacgtcttc
     9841 aatcgcggcg agcacccgat gatggtgttc gatcgcgacg gcaacttcct gcgctcctgg
     9901 ggcgagggca gctacccgcg cgcgcacggc ctgcacatcg acgtcgacga cacgctctat
     9961 ctcaccgacg atggcgggca cttcgtgcgc aagtgcacgc ccgagggcag ggtgctcctc
    10021 gagctgggcg tgccgggcaa gcccgcgccg ttcatgagcg gcaagccctt tcaccgctgc
    10081 acgcataccg cgctctcgcc cgagggcgag atctacgtga gcgacggcta cggcaacgcc
    10141 aaggtgcaca agtactcgcc cgacgggaag ctcctgctct cctggggcga gccgggctcg
    10201 gaccccggtc agttcaacgt ggtgcacaac atcgtcaccg acgccgaagg ctgggtgtac
    10261 gtcgccgacc gcgagaacca tcgcgtgcag gtgttcgacg cccgcggcag gtacgagacg
    10321 cagtggaaca acatgcaccg gccctgcggc ctgtactgct gccgcgggaa gcagctgcag
    10381 ttcatcatcg gcgagctcgg ccccggcatg aacatcaacc gcgaccatcc caacctcggg
    10441 ccgcgcctgt cgatcgtcga ccccgagggc aggctcgtcg cgcgcctggg cggcgagcag
    10501 ggcccgggac tggagaccgg aaagttcatc gcgccgcacg gcctcgcggt ggactcgcgc
    10561 ggcgacatct atgtcggcga ggtgagctac actgaatttc cgcgtctgta tccggagcgc
    10621 ggcatcccga aaggcctgcg ctcgctgcag aaactcacca aggtcgcctg aacaagggag
    10681 aacgcatgat ccacgtcatc gccgtcatca ccgccaagcc cggcaagcgc gaggagatcc
    10741 tggcgcactt tcgcgccaac gtgcccgcgg tgcgcgctga gaaaggctgc atcgaatacg
    10801 gccccgccgt cgacgcggac cccgcgccgc agatgcagac caagtacggg cccgacacct
    10861 tcgtcgccat cgagaagtgg gaaagcatgg acgcgctcaa ggcgcacgcc gccgcgcccc
    10921 acatggcggc ctacgcggcg aaggtgaagg acatgatcgc cggccgcgtg atccacattc
    10981 tcacgccggc ttgagcggct tggactcgag cagccgcacc agggcggcga gcgtgcggtt
    11041 cgcgggcgtg ggcacgccca gcgaagcgcc gcggcgcgcg acgaacccgt tcaagtgctc
    11101 gatctcggtc ggcttgccgc gcgcgagatc ctgcgcggtg gaggagtact gcgcgggcat
    11161 cgcctcggcg atcctgtagg tgcgttccag gatgtccggc gccatggtga cgccgctcgc
    11221 cctggcgacg gcgagcgttt cctcgacgac ctcgcgcatc acgtcgcgaa tgcccgggcc
    11281 ctcgatcatc ttgccgtagg gcagctgcga gatggcggag agcgcgttgt aggcgcagtt
    11341 cacgaccagc ttcgcccaca gctccccggc cacgttgccg gagatcgcga ccgggacgcc
    11401 ggcggcctcg aaccagcgct tcacctgcgc cagcgcgccc gccggaagct tcgcgttgag
    11461 ctcgccgatg acgaggtcgc cgcgcccgtg gtgcttcagg tggcccgggc cggccatctc
    11521 ggtggccacg tagaccaccg cggggatgac cggctgcgcg atgcgcgccg cgatgcgctc
    11581 ggtgttgtcc acgccgttct gcaggttcac gacgagcgcg ccggcgtcga ggtgcggcgc
    11641 catctgctcg gccgcgcttt ccgtgtcggt ggacttgacg cagaacagga ccacgcggca
    11701 cccgcggacc gcggatgcct cggtgctcgc cccgaccgca acctgctcgt cgaacttcag
    11761 gccctcgaag cgcaggcccc gcgccgcgaa cgccgcgacg tggaccggcc ggccgatgag
    11821 cgtcaccgca tgcccggcgc gcgccagcat gccgccgtag tagcagccca ccgcgcccgc
    11881 cccgagcacc gcgacctcga tcgactcgcc ttcagccatc ggcgccaata tatactggac
    11941 cgatgaccat ccattcgctg accgcccgcc gcgccgccga tcccgtgcgc gaggcgaaga
    12001 tccatctcgc cgcggcgcac cgcctggccg tgctgcacga gctggaggag gggatcgaca
    12061 accacttcac cgtgaccgtc cccggcgcga cggaccgcta cctgatcctg cccttcggcc
    12121 tgcactggtc cgaggccagg gcgagcgaca tgatcgtgtt cgacgagtcc ggccggacgc
    12181 tcgaaggcga gggcgtggtc gagctctccg cgcagtgcat ccacgcgccg atccatcgcc
    12241 tgaccggggc gcgcgtcgtg ctgcacacgc accagacctg ggcgctcgcg ctcaacatgc
    12301 tcaaggagaa ccgcctgctc cccgcgagcc agaccgcggc ctacttccac ggccacgtcg
    12361 cctacgacga cacctatgcg ggcacggcgg acttcgtcga ggagggcgag cgcctcgcgc
    12421 gcctgatcgg cgacaagcac gtgctgttca tgaagaacca cggcgtgctg gtcgtgggcg
    12481 acacggtcgc gcaggcctat cggcgcatct acaagctcga gcgcgtctgc cgcgcgcagg
    12541 tgctggcgat gggcaccggc aagccgctcg aagtgctctc ggacgagatc gcggcgcagg
    12601 tgcaatcgcc gcccgtggac gagcgccact cgcgcgccga gcgcgagcgc ctgtacttcg
    12661 aggcgatgat gcgggtgctc gaccgggaga atcccggcta cgccgactga ctccgggcgc
    12721 gagtccctcg ccgcggccgc cacgcgcggc ggcgatgccg ttcatcatgg cggcgacgct
    12781 gatcgacatg atggcgatcg gcatcatcat tcccgtactg ccggcgatgg tcggcgagtt
    12841 caccggctcg caggtgcacc aggcctactg gtatggcgtc gtcaccttcg ccttcagcct
    12901 ggcgaatttc ttcggttcac cgttcctcgg cgcgctgtcg gacagccgcg gccgccgcac
    12961 ggtcctgctg ctcgggtttt gcggcatggc gttcagcttc ctcgccaccg tgctcgccac
    13021 cgcgctgtgg gtgctcgtcg cggtgcgcgt ggtgagcggg gcgatgcagg ccaacatttc
    13081 cgtcgccaac gcctacgtcg ccgacatcac gccgcccgag atgcgcgcca agcgcttcgg
    13141 catgctcggc gcgatgttcg gcatcggctt cattctcggc ccggtgctgg gcggcctgct
    13201 cggcgcgatc gacctgcgcc tgccgttctt cgtcgcgggc accctggcgc tgtgcaatct
    13261 cgcctacggc tatttcgttc tgcccgagtc gctgccgccg gagcgccggc gcgcgttcga
    13321 gtggcgctcg ctcaatccgg tgggcgcgct gcgcggcctg gcgcagaccc ggggcgtggg
    13381 tgtgctggtg gcggtgattg cgtgcagcgg cctcgcgcaa ttcgtgctgt acaccacctg
    13441 ggtgctctac acgacgttca agttcggctg ggggccgctg gaaaacggct ggtcgctggc
    13501 ggcggtcggt ctggtttcgg ctatcgtgca gggcctgctg ctcggccggc tgctgcggat
    13561 cttcagcccg cggcgcctgg ctgtgctcgg tttgctgtcg tcgaccgtcg cgttcgtgct
    13621 gtggggcgcc gcgaccgagg gctggatgat gttcgcgatc atcttcgtca acctgctcgg
    13681 cgtcaccgtc gcggcctcga tccagagcct gatctcgcag gcggtggacg cacgtagcca
    13741 gggacaggcg ctgggcgccg cgggcggcct caacagcctg atggcggtgg tcgcgccggc
    13801 gatcggcgcg ccgctcctcg cgctggtgtc gcacctgccc cagggcgact ggcgcatcgg
    13861 aacgccgttc tatttctgcg ccgcgctgca ggcggcggcg ctcgcgctcg ccgtaatcca
    13921 ctttcgcggc gaacgccgcg cgcggctcgc cggctagccg cgccgcgcgc cgtagaactc
    13981 gcgcagcagc ccggccaccg cttcgggctg ctcctgctgc acccagtgtc ccgcgccatc
    14041 gacgaggtgc gtgcccaggt agcgcgcgca ggccttgtcg cgcatgtcct cgaggttgcc
    14101 cggccgctgg taggggcccc agtcgctctt gcccgcgatg aagcaggcgg gaacctcgat
    14161 tctccgtccc gcaaagagct cgaacaggcg cggcagcggc ggctccacgc gcgcccggta
    14221 ccactgcaag ccgccctgca gcccggtgcg gccgaactcc gcgctgtaga cggccagttc
    14281 cgcctcggtc agccagcggc aggcggcgat ttgctcgcgc gaaggcatct cgtgcgcgac
    14341 cgtctgcgcc atggtctcgt gcaggtccat gacgtagtac gtgggcatgc gcgcgagctc
    14401 ggcggccgtc cagccagcga gcggcgccgg gcggttctgc gtccagtcgg cgctcttcat
    14461 gtggaaatag gcgcgcagga acgcgtgcag cccctgcgcg cagtgcgtca tgtcgcggtt
    14521 cgcttcgcgc gtgcggtagt aggcctggta atgcttgcgc gggcggggga gcgccgcgag
    14581 cgcctcgtgg atcgggtcgg gcgcttccgg caccagtccg ggcggtcccg tgaaaggcgc
    14641 gctcatcagc gccaccgcgc ggaacacgtc gggccgcacc agcgcgcacc acgcagcgac
    14701 ccaggagccg aagtcgtgtc cggcgaggtg cacggtgtcg tggcccagcg cgcgcaccac
    14761 gcccagcgcg tcgcgggcga gattgagcag gtggtaggag gcgagatcgc cgtcgtagtc
    14821 cggatcccag ccggtggtgc ggccgtagcc gcgctggtcg ggcgccatca cgtgataccc
    14881 cgcttccgcg agcggcagca tcaccttgcg ccagctgaag gcaagctccg gaaacccgtg
    14941 cagcagcaac aggccgggtg cgcgcgggtc gcccgcctcc agcacgtgcg tgcgcagccc
    15001 gttgttccgc tcgaccaagc gcgagcggat gcccgccgga agcggcagcg cccgcaaggt
    15061 cagcgcgcgc gctttctcgc cagcgcggcg ccggcgccca gcgccttcag cttcgccagc
    15121 gcgatgtcgc gccgcatcgg cgccatgccg cagttggtgc agggaaacag gtgcttcctc
    15181 ggcacgtact tgagcgcgtt gcggatggtg gcggccacct cctgcggcgt ctcgaccttg
    15241 tcggaggcga cgtcgatcac gcccaccagc acgtccttgc ccttcaagag cttcatcagc
    15301 tcgggcggca cgtgcgagtg tatgcattcc agcgacactt gcttgatctt gctcctggcc
    15361 agcgccggaa agatcttctc gtactggcgc cattcgccgc cgagcttctc tttccactcg
    15421 aggttcgcct tgatgccgta gccgtagcag atgtgcacgc aggtcgtgca cctgaggccc
    15481 ttggccgcgc gctcgagcgc cttgatgccc cagcggttca cgtcgtccat gtagacgttg
    15541 aacgccggct cgtcgaactg gatcacgtcg acgccgtcct tttccagcgc gcgcgcctcc
    15601 tggttgagca actcggcgaa ggccatcgcc agcttcacct tgtcgccgta gtggcggtcg
    15661 gcgatggtgt cgacgatcgt catcggcccg ggcatggtga acttgagctt cttcttggtg
    15721 tgggcgcgcg ccaggcgcgc ctcggcctgg tgcacgcggc ccttgagccg gatcggaccc
    15781 gttaccaccg gcaccatggc ctcgtagcgg ttgtcgcgga tgcccatctt caccttgtgc
    15841 tcgaaatcga tgccctcgac gaactcgagg aagccgtgca cgaagtgctg gcgcgactgc
    15901 tcgccgtcgg aaacgatgtc gatgccggcg tcctcctgct ccttcagcca cagcaacgtc
    15961 gcgtcgcgct tggccttctc gagctccgcg ccgctcgcct tccacgcggg ccagagcttg
    16021 tcggtctcgg cgagccagaa gggcttgggc aggctgcccg caaccgtcgt ctcgagcatc
    16081 cgcgtctccc tctttggccg cgccagatta cgcgtgatcg ggcgggccat cgatggaata
    16141 gtgcgcggtc acgaacacgc ggtcgacaac ggcgggacg
//
LOCUS       DBNHKE010000216        27760 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1204072, whole genome shotgun sequence.
ACCESSION   DBNHKE010000216 DBNHKE010000000
VERSION     DBNHKE010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 27760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27760
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1204072"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="AC16O9_14680"
     CDS             <1..826
                     /locus_tag="AC16O9_14680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR6517897.1"
                     /translation="GELMRLPRAGVAVRFGEKLLEDLDRALGRVPEPRAYFHPPERFD
                     MQLELPAPVMEAERVLFAARRLLLQLEGFLAARQAGVRGFTLHLSHRDGRVTRIEIGS
                     ATPHRDIEHWQRLLRERLEATAVKSPAEAIRIEAAGLTPFSGVSSGLFGDTRSEAEAW
                     ERLTERLQARLGGTQVHGLATHAEHRPERAWRTLAADAAAADDAAPGPRPLWLLEPPR
                     RLGDGEFELLAGPERIESGWWDGDDVRRDYYIAACGSSLGWIFRGGEGWFLHGLFA"
     gene            810..3842
                     /locus_tag="AC16O9_14685"
     CDS             810..3842
                     /locus_tag="AC16O9_14685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HIR6517898.1"
                     /translation="MDCLPEYAELHCLSNFSFLRGASHPEELVSRAAALGYAALALTD
                     ECSLAGIVRAHVAAKEHKLKLIAGTELRLEDGVRLALLAADRRSYGALCSLITTGRRR
                     AKKGVYILARADVEACAASGLLALLLEGDAHWLAGHFPGRAWIAAELHCGPNDRTRLE
                     SLRALSRASGLPLAAAGDVHMHLRSRRRLQDALTAIRLGTPVERCGRALFPNAERHLR
                     ARVRLAQLYPPELLAESMAIAGRCTFSLDELRYEYPEELVPEAKTASAHLAELTAQGL
                     AWRFPAGVPQKVRALVDYELRLIAELRYETFFLTVHDIVRFARGRGILCQGRGSAANS
                     AVCYALGITEVDPGRLQTLFERFVSKERNEPPDIDVDFEHQRREEVIQYLYAKYGRGR
                     AALAATVICYRPRSAVRDLGKALGLAPEQVERAARSLAWWDGRQVLPERLAESGLDSS
                     SPALQRLVTLTGELVGFPRHLSQHVGGFVISRGPLAELVPVENAAMPERTVIQWDKDD
                     LEALGLLKVDVLALGMLSAIRRALDFLGMQMHEVPAEDPAVYAMIQKADTIGVFQIES
                     RAQMSMLPRLRPACFYDLVIEVAIVRPGPIQGGMVHPYLRRRRGLEPVVYPSAAVQGV
                     LERTLGVPIFQEQVMQLAVVAAGFTPGEADRLRRSMAAWKRKGGLEPFRERLAQGMRE
                     RGYPREFADAIYRQILGFGEYGFPESHSASFALLVYVSAWLKCHHPAAFCAALLNSQP
                     MGFYAPSQLVQDARRHGVEVRAPDVCVSAWDCALEGGALRLGLRLVGGLAEVAAAKIV
                     AARAQGAFASVEDLARRAALNRRDLRCLAAGGALAGLAGHRRQAHWTAAGIVQAAGVL
                     REAPIDEPSLALPPPSEGEDIVADYASLGLSLGRHPLALLRPRLSRLGLLPAAAVHAR
                     AHGSTARAAGLVTCRQRPDTASGVIFVTLEDETGCVNVVVWRDLIERQRRALLGARLL
                     AVEGRIERDGEVVHLIAGRLSDRSALLGQLLAPSRDFR"
     gene            3917..4222
                     /locus_tag="AC16O9_14690"
     CDS             3917..4222
                     /locus_tag="AC16O9_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6517899.1"
                     /translation="MSKPNPTAIIAKDLADLIPGFFTNRNKELELLRGALAGADFEQL
                     RHLGHRMKGVGASYGFTTISEIGKRIEDGARSSNRGVIDACIGEYADYLATVQVVYE"
     gene            4215..5369
                     /locus_tag="AC16O9_14695"
     CDS             4215..5369
                     /locus_tag="AC16O9_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6517900.1"
                     /translation="MSRSSADRRRAPRSAPAPFRVLVVDDDVDMARFLGRLLAQEGMK
                     VQTALSGETGLASIAESPPDLVLLDVMMPGTNGFDVCRRLKSDELTALIPVVLVTSLE
                     DSESRVRGIEAGADDFLSKPVKREELIARVKTLRRLHETRKELESRRLAAEVQRKEAI
                     RKAFSRYISPRLADRIAEAAGEQGDPFQGRAQRTNLVALFADLRGFTRLTEKNEVSAV
                     VGMLNEYFAILTEAAYQHDGTIFNMAGDSLLVGFNVPFPQADAAERAWHTALEMVGRF
                     APVAAGWHSTLGLELGVGVGICAGEAIIGNVGSEHYASYTIIGNPVNTAARLMQMAAA
                     GEVLVCGPVFERIRHLLSGHRVDGRGDVALRGRSQPIPVFAVRAVAERTA"
     gene            5366..6598
                     /locus_tag="AC16O9_14700"
     CDS             5366..6598
                     /locus_tag="AC16O9_14700"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6517901.1"
                     /translation="MRVLIIDDDPDLRNLLAHYIRQQWPGGEVEEYDPLERDKPDARF
                     ALGAYDVIILDYMLGRGDGLQWLSEFKKRADCPPIIFLTGAGNEIIAVRAMKAGADEY
                     QRKQELTKERLITAIRDVAHDRIEKTVPPEVAARMEGHILGARIRIPGIKVLRLIGEG
                     GMSRVYLASRESDDEPLVVKILRSEVTQDKRALARFIEEYNLVERISTRHVAHIHGHG
                     VSEDNAYLVMEFFDGGDLNKRLAGKALPAEEALRVFRELMFALGDIHEQGILHRDLKP
                     QNLMFRADGSLAIVDFGIAKHIDSIDHTNKGEILGTPRYMSPEQVQGRALDLRTDIYS
                     AGVLLYQMMLGSHLFEGETAVEVALHHLNTPPPGLPERLQRYQRLFDKLLEKDRDARF
                     RNADEVIGYLSRKFYQGQ"
     gene            6617..7822
                     /locus_tag="AC16O9_14705"
     CDS             6617..7822
                     /locus_tag="AC16O9_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6517902.1"
                     /translation="MRKKIPVEELQLGMFVGELDRPWIGTPFLYQGFVVREPQELEAL
                     RKYCREVYIDTEKADRSVARPASRAAIPGLPGTGRIEHRETVAVEVEWPRARDALEGA
                     GTALTDVFESVRVRKLLDATQARSAVGNMTQSMLRNPDALMLFAAMRHRGGYQLERAM
                     DVSVYMIAFARFLGMDEEDIERAGHVGLLQDIGMLDLPQEMLQKVTRLEPAEFKIIRG
                     HVARGREVLAGTSGLPADVAQIAALHHERYDGSGYPGGLKGDALGVIGAMAGIVDTFD
                     ALTVKRPYAEAMSPSNALNLLFRMRGRSFHPQLVEQFIRCVGYFPVGSIVELNSGEVG
                     VVIAQNAEQRLQPKVMVVRDAGGQPLRPQKFLNLARLPKASGAEPYRIRRTLEFGRAG
                     ISVDEVLVG"
     gene            complement(7829..8218)
                     /locus_tag="AC16O9_14710"
     CDS             complement(7829..8218)
                     /locus_tag="AC16O9_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6517903.1"
                     /translation="MPDRPLLRICYVEDDEDIQRVARLALERVGKMEVSIIGDPTVAL
                     EGILAFKPDLVLLDWMMPVMDGPTLFKRMRETDGLRDLPVVFITAKASQRELDELREM
                     GAAGVLSKPFSPKDLPEQLRAIWRTLP"
     gene            complement(8231..10051)
                     /locus_tag="AC16O9_14715"
     CDS             complement(8231..10051)
                     /locus_tag="AC16O9_14715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6517904.1"
                     /translation="MTSGSAAPVTLAPTREASARAEDAPGHADPQRVLADRVEQLYGQ
                     MPLGMLAHLLLGGIATYELWDQRTRDLVLFWWGLVLLVTAARAVLYYGYRASGNKLVE
                     ADQWMRWMAISALASGVTWGFAGAVFFPSHPDEQQVFLAFLLAGVTSAGIPAYAASWP
                     MFAMYAGAIVLPFTYMLGTFGNRLFTEIAVLVPIFYLINVGIAYRLNRVFLSGYRLRH
                     AYDRLTEDHTRLNKQLEQQIEDLLEARQEVEASGRKLTLFAERAPISVIEMDANSTIL
                     EMNPAAEHMLGYSTLELIGRSALNTLFPADEATLTPQWWAGFVARREPVTRLRVRCLR
                     RDGLEIMCEFTITPLVSESGDLVSIIAQCQDITQQLEAERLKKEFTSTLSHELRTPLT
                     SIIGSLQLVNSGVVGDASGEIAELSSVAERNAQRLLDLINDLLDIDKIESGKFTLQPE
                     RMRLDELVQESLVLNKSFADRFRVALEVHGELPDVQVHVDRKRLMQVMTNLLSNAAKF
                     SPEGSAVEVSTRCSEGMVRVAISDRGPGIPEDFRSRIFTRFAQADSTTTRHKGGTGLG
                     LAICKRLIEMMDGRIGFEDRPGGGTTFHFELPVMADAVST"
     gene            complement(10110..10745)
                     /gene="lexA"
                     /locus_tag="AC16O9_14720"
     CDS             complement(10110..10745)
                     /gene="lexA"
                     /locus_tag="AC16O9_14720"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR6517905.1"
                     /translation="MGAIEPLTERQGEILKLIRELTESSGFPPTRAEIAEKMGFRSVN
                     AAEQHLRALERKGALEILQGASRGLRVKEKSAPRHGRLMELPVVGRVAAGSPILAEEH
                     LQGRYQVDPNLFTPRADYLLRVRGMSMRDAGILEGDLLAVHRTQEARSGQVVVARLAD
                     EVTVKRYRRRGHAVQLLPENPDFTPIDVDLRRDPLTIEGVAVGVIRNGRGL"
     gene            10825..11682
                     /locus_tag="AC16O9_14725"
     CDS             10825..11682
                     /locus_tag="AC16O9_14725"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HIR6517906.1"
                     /translation="MAAIAVHGGAGRWMASDRTLAVAGVARAAAVGWHVLAAGGSAFD
                     AVVEAVASLEDDPIFNAGTGSVLNARDDVEMDASVMAGDTLAAGGVACIQRVRNPVLV
                     AKRVLQVSDCVLLAGPAATAFAREQGFEDYDPGRPRAAHAGRRGGTVGAVALDDAGRL
                     AAATSTGGREGKPPGRVGDSPVPGAGNYADAAAAVSATGWGEQMLRYVTAKDVATRIA
                     RGALPEPALREALDDMARRFGEPIGAIALDARGRLGVVHGTEAMPHAWRRSGEPEVTA
                     RFAAGRIPA"
     gene            11679..12860
                     /gene="pncB"
                     /locus_tag="AC16O9_14730"
     CDS             11679..12860
                     /gene="pncB"
                     /locus_tag="AC16O9_14730"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR6517907.1"
                     /translation="MIIRSLLDTDLYKFTMMQVVLHHFPGAQVEYRFTCRTPGVNLVP
                     HLEEIGAEIADLCRLRFRDEELAYLRGLRFMKSDFVDFLGLFQFNQKYIRLAPLGKTG
                     EVDITIRGPWLHTILYEIPVLAIVSEVYFRRTQPGADLAEGRKRLAQKIALVRKVEPE
                     LEFRISDFGTRRRFSLAWHEEVVQTLKSEVPQYFAGTSNVLLAMQHRVTPLGTLAHEY
                     MQACQALGPRLRDAQVFALDKWAQEYRGDLGIALSDTYGTDAFLRDFDLYFCKLFDGA
                     RHDSGDPFAWGEKMIAHYQKNRVDPHTKTLIFSDQLSFPLAIEIARRFHGRARTAFGI
                     GTNLTNDVGFEPINIVIKMTGCNGQPVAKLSDAPGKTVSTDPGYLAYLRRVYGLEPAP
                     R"
     gene            12857..14074
                     /locus_tag="AC16O9_14735"
     CDS             12857..14074
                     /locus_tag="AC16O9_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517908.1"
                     /translation="MTGQGRTAVAVLATVFLFNLLGRGSGDTYAVFLLPLEREFGWAR
                     SQLTSVYSMYLLVGAFMAPLAGTLFDRFGPRVTYSGGLLCLSVAFFLASTLGNLWEFY
                     LFIGVTIGVGVGLVGMVPASALLTRWFRARLSSAIGIAFSATGTGVLLFVPFSQWLIG
                     QLGWRGAYQVLAGLVFLAVPFALFVVPWRLYVAGLQDSVSGLRARAGGEGVTVRAAVR
                     TRTFWALVRVFFFTATAMFIVLVQAVVYFIDLGFSPITAATAFGVVGMLSVVSVSTSG
                     LAAERFGYRNTVSASFAGTALGVGLLAVMSAWPSGTLLAIFVGVFGLCMGVRGPIVSS
                     IATRRFAGPKVATIYGLIYAANAVGAAFGSLMGGVLHDLSGGYRAGFALSVCAVGLAV
                     APFWTVKELREFR"
     gene            complement(14058..15209)
                     /locus_tag="AC16O9_14740"
     CDS             complement(14058..15209)
                     /locus_tag="AC16O9_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR6517909.1"
                     /translation="MQLSRWIRPGDGVIFGQACAEPQTLVEALVAQRAEYTGATLFMG
                     VNWSGTVKPEHADHLRLTAYCGSGLNRKLADAGVLDIHPHPYSQLATLIRQKRIRADV
                     VLVQVSPPNARGEYSLGLSAEYLVPALEVCRSVIAEVNERVPWTFSERLLKKSDFAAM
                     LETSREPVTLPYGAPGPQELAIARNALEFIPDGATLETGLGALPDAVLQLLGDRKDLR
                     IHSGSVGDGVAALMQKGVVAGVQGGTLMGTRRLFDFAHENPKLRLRSIEYTHNARVLG
                     GIERFVAINSAIEVDTTGQVNAEVAKGSYLGAVGGALDFVRAANQSPGGVSLMLLPAA
                     RIVETLQGPVATPRSEAGVIVTERGAADLRGCTLRERLERMRAIASGTP"
     gene            15244..15834
                     /locus_tag="AC16O9_14745"
     CDS             15244..15834
                     /locus_tag="AC16O9_14745"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HIR6517910.1"
                     /translation="MLDDKAQDLIFREAHTPQGFLDSPVPDAKLKQVYDLMKWGPTTM
                     NTQPMRIVFVRSKAAKEKLAPALSSGNLDKTMKAQVTAIIAYDSRFYENLPKTFPNNP
                     KAKDNFAGEEKKASNDRVALRNGSLQGAYFIIAARALGLDCGPMSGFDNAKVDAAFFP
                     DGRCKSNFLINLGEADPAKVFPRNPRLSFDEACRIE"
     gene            complement(15837..16664)
                     /locus_tag="AC16O9_14750"
     CDS             complement(15837..16664)
                     /locus_tag="AC16O9_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR6517911.1"
                     /translation="MTQLPFKQVDVFTGRPFLGNPVAVVIGAEGLDAKQMQRIAAWTN
                     LSETTFLLRPTQSAADYRLRIFTPRRELPFAGHPTVGSAHAALESGFVKASNGTLRQE
                     CGAGILELALEEQRIYVRAPAPKVSAATVPLFGNARALRVDVGPVWVVVDLGDAGAVD
                     GLAPDMTAIAELSDALDATGVTVFGRTGNKAAPIHVRSFAPAHGIPEDPVCGSGNVSV
                     GAFLRETGLLKEFGASYTARQGMQVGRDGRVAVRVTADAIEIGGEAVTCVDGTLRVA"
     gene            complement(16661..17041)
                     /locus_tag="AC16O9_14755"
     CDS             complement(16661..17041)
                     /locus_tag="AC16O9_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR6517912.1"
                     /translation="MAQAALFLIFFAAPLLAHAQSPQAELRCKATGEDFVYDCIITLS
                     RGGRPLAGAEMAVAADMPTMPMAHNVRPARARPGAKPGEYKARLDLEMLGEWTIKLRL
                     DSPVRDLLVLHYEFDSKGATPRKR"
     gene            complement(17017..17904)
                     /locus_tag="AC16O9_14760"
     CDS             complement(17017..17904)
                     /locus_tag="AC16O9_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517913.1"
                     /translation="MDDYAIGVLTNIGLFSFVALSAYLLLIAGEMSFGQQAFFGVGAY
                     AGGILTALYGWPLPLAAFAAMGLGALAQLLVALPTLRLRGLYFAMATLAAAEMARILF
                     ELLHWRRKVDGELAGPDGTQGFRGIRWIFEHGIEPLHYLGIVGVLLALVLALLLVVER
                     SKLGATLRGVGEDPELAASVGIDVQRVKLAVAAAAGALAALGGVLYAHHNTYIEPRNF
                     DIMLGVHSLAYALIGGLGTAFGPLLGVLLDIGVLESTRIFQGYRMIVFGGLVAVVLIY
                     RPRGLLDERIVQWLKQRFS"
     gene            complement(17877..18749)
                     /locus_tag="AC16O9_14765"
     CDS             complement(17877..18749)
                     /locus_tag="AC16O9_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517914.1"
                     /translation="MLADFLQNTLDGLSAGASYALLALGFTLIFGVLRRANLSFGPSI
                     MFGAYCATWAHVNFGAGLLLLALISVGGAVLAGAYVERLCFAPHRGGSAIAAMVASFA
                     LWMQFEELATLVMPRHLYAFPMLYEGDPLSLGPLTLRVDTLAMLAAALAACALLWWTL
                     YRTRFGLAVRALIEQPAAAALAGINVPWVFMRVFALASALGGIAGYLVVATHGQITPM
                     LGMWATLKGLLAMMLGGLGSLPGAVIGGLALGLIEAHTQWYLGPQLRDLVAYLLLFVL
                     LAARVPWMTTRSAS"
     gene            complement(18771..19490)
                     /locus_tag="AC16O9_14770"
     CDS             complement(18771..19490)
                     /locus_tag="AC16O9_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517915.1"
                     /translation="MNFLSVQDIVTAYGKIEALRGVSLEVAEGAITCLLGPNGAGKTT
                     LMLTLAGILRPKRGSIRFAGEEIAGRAPHAIVARGVALVPENRLVFPAMTVAENLQAG
                     AYHRRDKATVRSDMEGMFARFPRLKERAAQQAGTLSGGEQQMLAVARALMSRPKLLLM
                     DEPSLGLAPLVVEEIFRIVAELHRDGTTIFLVEQNAHMALKVAQHFYLMEQGRVTFSG
                     DPGSLAEDEVIQRAYLGSVQR"
     gene            complement(19487..20236)
                     /locus_tag="AC16O9_14775"
     CDS             complement(19487..20236)
                     /locus_tag="AC16O9_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6517916.1"
                     /translation="MLELRSLSRHFGGVRAVDGLDLTVADGQIFGLIGPNGSGKSTTV
                     NVVAGLFAPTAGAVLFRGRDIGALPTHLRLREGIARTFQNIRLFGQLSVWQNLWVAQN
                     SAADRARQGFFSRWLGGQAGVREEIGRLLEFSGLAHKQNELAANLAFGEQRRLELARA
                     MAAKPRLLLLDEPAAGMNSEEIGQLRERILKLRASGVTILLVEHVMELVMGVTDRIAV
                     LNFGQKIAEGTPAEIQADAAVRKAYLGGGAA"
     gene            complement(20229..21095)
                     /locus_tag="AC16O9_14780"
     CDS             complement(20229..21095)
                     /locus_tag="AC16O9_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517917.1"
                     /translation="MDFYFGLAQLIGVHTLLGLSAYIVLLTGQVSLAQAGFFAVGAYT
                     AGMLTVLAGWHLVPALGVSALTGASVACAVGFPALRVKGLMLVIATLAFGEGVRLFFF
                     NFDYQVAKGNLKVGPLGGEGFRQIRFFPENGWETWDVMLLIWVFVVAAMALLWWFDRS
                     RYGSVLRAVGEDELAAQSVGINLTAVKVSAMTVGGAIAGVGGGLYAHYTTHIEHASFN
                     ILVATFAIAYPIVGGLRNVFGTLLAVIFVQGFLVEGLRFLGDWRNLLFGALIVLAMNL
                     RPSGILGRRRHA"
     gene            complement(21098..21976)
                     /locus_tag="AC16O9_14785"
     CDS             complement(21098..21976)
                     /locus_tag="AC16O9_14785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6517918.1"
                     /translation="MLDLLQTVSDGVLFGATYALIGIGFTLVFGVMHKINMAYAAASV
                     GGAYAGLLVLTVMAQAPPALVFFGAFVAGGVLGWVVYLTCFHFIPLSSPLATLMSTVG
                     MLLLIDEVIVHVTAGMPQSYPAMFDDVMLRAGEFSMRGDLLLVFGVSVLAMIALLLLL
                     YRTSLGLATRAVSQQPVAAQLCGIRLVRVNALTFVVTGLLGGLAGALIGASVGTLSPL
                     LTTPLTVKGLIVTVIGGLGSIPGAIVAGLLVGGFENVLQLYRGVTERDLYVMLLLFVF
                     LTFRPGGLFAPKGGRD"
     gene            complement(22031..23203)
                     /locus_tag="AC16O9_14790"
     CDS             complement(22031..23203)
                     /locus_tag="AC16O9_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6517919.1"
                     /translation="MKTLALAVSLALAAGVAHAQTIKIAGFGAQSGPVRSFGVNSEAA
                     LRAAADVVNKAGGVKLADGSKAKIEIEFYDDRCNAEEGISVIRRIAGTDAVVAVGPTC
                     SNVAEPLFGILQKKVGDSGDSGLQMPVFTDVAIKVGLAKISEWAFRNVPNEMTMYNSL
                     FYWLKTKHPELKTVYGGVEEDFAHSRATWYVVMKDRAAASGFEVLGNTKWLLADTNFA
                     TQVREMKKANADVIAISAHPFSACGALKEMQRQGVKPKLLVGLTSSSSLETLQGCAKQ
                     AEGIIIPTSFAPINKDATFAAKTTAKFKGAADLHSMAAWENVFILKQVMESEGISGKP
                     GDVQSDRRKIRDGLAKLRTTDGLIGTSKRDAEGEADKPYVFVQARKGEWKVLHNPL"
     gene            23259..24914
                     /locus_tag="AC16O9_14795"
     CDS             23259..24914
                     /locus_tag="AC16O9_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6517920.1"
                     /translation="MAKKKRVVAVRNTDPWAALKPGAANRQELSPLSFLPRAAEIYPQ
                     RVAVIHGAARITYAQFFERARRLASALARGGVGRGDVVSAMLPNVPAMLDAHYGVPVL
                     GAVLNTINTRLDADTVAYILEHAGAKVLITDRVFAGVVGPALKKLKNKPFVVEVDDPL
                     YSGPGERLGSMEYEKFLAGGDAGFAWRQPRSEDDPIALNYTSGTTGRPKGVVYHHRGT
                     YLEALGNIVAWAMPAHPVYLWTLPLFHCNGWCYPWSVTAMAGTHVCLRAVDPALIFPM
                     IVEHGVTHMCGAPTVLTMLISAPHEQRRPFAGPVHIMTGGSPPPAKVIRDMEELGFKV
                     LHIYGMTELQGPSTFCAEQDAWAALPLEARAVEMARQGVRYQVVEGHIVGDPQTCEPV
                     PKDGRTLGEILVQGNTVMLGYLKDAKATAEAFHGGWMHTGDLAVWHPSSYIEIKDRKK
                     DIIISGGENISSVEVEIALYKHPATALAAVVARPDEKWGETPCAFVQLKPGAQATEEE
                     FIQWCRDNLARYKVPRKVVFGNLPTTATGKIQKYVLRETARTL"
     gene            complement(24918..25811)
                     /locus_tag="AC16O9_14800"
     CDS             complement(24918..25811)
                     /locus_tag="AC16O9_14800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6517921.1"
                     /translation="MVSLARYAWLSVATALVTMALKGIAAWLTGSLGLLSDALESFVN
                     LGAGLIALYTLRVATRQPDEKHPFGYTKAEYFSSAVEGGLIVVAAIAIVAAAVPRLIE
                     PQPILRFDVGIFISLLAAALNLGTALVLLRAGKRHDSIALEADAHHLLTDVWTSIGVV
                     AGVGATVLTGWLALDPLIAIAVAVNILWVGARVMRRSVLGLMDRALPEPDLERIRAIL
                     DGYRADGLDYHALRTRRAGRLRLVEMHLLVPGEMSVREGHRIADEIEEKIRAKLPGAA
                     VMTHLEPIEDPASYRDDTLIR"
     gene            25994..26164
                     /locus_tag="AC16O9_14805"
     CDS             25994..26164
                     /locus_tag="AC16O9_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517922.1"
                     /translation="MKLEQGKPDAAAATEKPLKTAAATGRRPTRINEDARHCLDLPTT
                     AEIVRCAEPYRY"
     gene            26246..27439
                     /locus_tag="AC16O9_14810"
     CDS             26246..27439
                     /locus_tag="AC16O9_14810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6517923.1"
                     /translation="MELDPRTLVVASVLAALLLGSVSIAFAQVQGSSHIIGAWGRAML
                     VLAIGLLGLALRDAIPLWLSAVVGNLLIVAAIALAMRALRTFVGSTPRDETSWALLAA
                     LFLMLLYLTEVRPSHIGRTIAISAALGIIAWRAARLLHLRAPANCKLSSQVTEYIFWG
                     VAALSFVRIVGTVLFPPVASLAAGPFNASVFLFYSGLIIVSTLSVMWMEIESLQGDLV
                     RAARYDALTKVYNRGTFLEEFEREVSRCARGSPAFSLAIFDLDRFKRLNDLYGHPTGD
                     RVLQAFADVLRADIRRHDVVGRYGGEEFALLMPNTRKETALRVVERVRRDLDARGITV
                     DGTRIEVTVSGGISTYGADGEDWGTLLTAADTALYEAKDAGRNRVVTAGAAPVPARAA
                     PSPPG"
     gene            complement(27436..>27760)
                     /locus_tag="AC16O9_14815"
     CDS             complement(27436..>27760)
                     /locus_tag="AC16O9_14815"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6517924.1"
                     /translation="ARASMVMDHGRSGPLGALGGAEGGVNKVAIEQSGSQYRPPHLSK
                     DQDIQLAPGDCIRVSTPGGGGYGDPLRRDPSKVALDVSRGYYTRAEAERLFGVRFAED
                     GSARR"
BASE COUNT         4440 a         9340 c         9625 g         4355 t
ORIGIN      
        1 gggcgagctg atgcggctgc cgcgcgccgg ggtcgcggtg cgcttcggcg aaaagctcct
       61 cgaagatctc gaccgggccc tcggccgggt gccggagcca cgcgcttatt tccacccccc
      121 cgagcgcttc gacatgcagc tcgagctgcc ggcgccggtg atggaggccg agcgcgtgct
      181 gttcgcggcg cgccggctgt tgctgcaact ggaaggcttc ctcgccgcgc gccaggcggg
      241 cgtgcggggt ttcacgctgc acttgtcgca ccgggatgga cgggtgacgc ggatcgagat
      301 cggttccgcc accccgcatc gcgatatcga gcattggcag cggctgctgc gcgagcgcct
      361 cgaggcgacc gccgtgaaga gcccggcgga ggcgatccgc atcgaggctg ccgggctcac
      421 cccgttctcc ggcgtctcgt ccggtttgtt cggcgatacg cgcagcgagg cggaagcctg
      481 ggagcggctg accgagcgcc tgcaggcgcg gctgggcggc acacaggtgc atggactggc
      541 gacgcacgcc gagcaccgcc ccgagcgcgc ctggcggacg ctggccgccg acgccgcggc
      601 cgcggacgac gcggcgcccg ggccgcgccc gctttggctg ctcgagccgc cgcgccgcct
      661 cggcgacggg gaattcgagc tgctcgccgg gcccgagcgc atcgaatcgg gctggtggga
      721 cggcgacgat gtgagacgcg attactacat cgccgcctgc ggcagctccc tggggtggat
      781 cttccgcggc ggcgaggggt ggttcctgca tggactgttt gcctgagtac gccgagctgc
      841 actgcctgtc gaacttctcc ttcctgcgcg gggcgtcgca tccggaggag ctggtgtccc
      901 gcgcggcggc gctcggctac gcggcgctcg cgctcaccga cgagtgctcg ctcgccggca
      961 tcgtgcgcgc gcatgttgcg gccaaggagc acaagctcaa gctgattgcc ggcaccgagc
     1021 tgcggctcga ggacggcgtg cggctcgcgc tgctcgccgc ggaccgccgc tcctatggcg
     1081 cgctttgcag cctgatcacg acggggcgcc ggcgcgcgaa gaagggcgtc tacatcttgg
     1141 cacgcgccga cgtcgaagcc tgtgcggcga gcggcttgct cgcgttgctg ctggaggggg
     1201 acgcgcactg gctcgccggg catttccccg gccgcgcctg gatcgcggcg gagttgcact
     1261 gcggacccaa tgaccgcacc cggctcgaat ccctgcgcgc gctctcgaga gcgagcggcc
     1321 tgccgctggc ggcggcgggc gacgtgcaca tgcacctgcg ctcgcgccgg cggctgcagg
     1381 acgcgctcac ggcgattcgc cttggcaccc cggtcgagcg ttgcgggcgt gccctgttcc
     1441 ccaatgccga gcggcacctg cgcgcgcgcg tgcgcctggc gcagctctat ccgcccgagc
     1501 tcctcgccga gagcatggcc atcgccgggc gctgcacttt ctcgctcgat gagctgcgct
     1561 acgagtaccc cgaggagctg gtgcccgaag caaagacggc gtctgcgcac ctggcggagc
     1621 tcacggcgca ggggcttgcc tggcgctttc ccgccggcgt gccgcagaag gtgcgcgcgc
     1681 tggtcgacta cgagctgcgc ttgatcgccg agttgcgtta cgagacgttc tttctcactg
     1741 tgcacgacat cgtgcgcttc gcgcgcgggc gcggcatcct gtgccagggc cgcggctcgg
     1801 cggcgaactc ggcggtgtgc tacgcgctcg gcatcaccga ggtggacccg gggcgtctgc
     1861 agacgctgtt cgagcgcttc gtgtccaagg aacgcaacga gcctcccgac atcgacgtcg
     1921 acttcgagca ccagcggcgc gaggaggtga tccagtacct ctacgccaag tacggccgcg
     1981 gtcgagctgc gctcgccgcc accgtcatct gctaccggcc gcgcagcgcc gtgcgcgacc
     2041 tgggcaaggc gctcggtctt gccccggagc aggtggagcg cgcggctcgc tcgcttgcct
     2101 ggtgggacgg gcgccaggtg cttcccgagc ggctggcgga aagcggcctc gactcgtcta
     2161 gcccggccct gcagcggctg gtgacgctga ccggcgagct ggtgggcttt ccgcgccacc
     2221 tgtcgcagca cgtcggcggc ttcgtcatct cgcgcgggcc gcttgccgag ctggtgccgg
     2281 tcgagaacgc ggccatgccc gagcgcaccg tgattcagtg ggacaaggac gatctcgagg
     2341 cgctcgggct gctcaaggtc gacgtccttg cgctcggcat gctctcggcg atccgccgcg
     2401 cgctggactt cctcggcatg cagatgcacg aggtgcctgc ggaggacccc gccgtgtacg
     2461 ccatgatcca gaaggcggac acgatcggtg tgttccagat cgagtcgcgc gcgcagatga
     2521 gcatgctgcc gcgcctgcgg ccggcatgct tctacgatct ggtgatcgag gtggcgatcg
     2581 tgcgtcccgg gccgatccag ggcggcatgg tgcatcccta cctgcgccgg cgccggggcc
     2641 tggagccggt ggtctacccc agcgcggcgg tgcagggcgt gctggaaagg acgttgggcg
     2701 tgccgatctt ccaggagcag gtgatgcagc tcgcggtggt cgccgccggc ttcacaccgg
     2761 gcgaggcgga ccggctgcgc cgctcgatgg cagcctggaa gcgaaagggc gggctcgagc
     2821 ctttccggga acggctggcg cagggcatgc gcgagcgggg ctatccgcgc gagttcgccg
     2881 atgccatcta tcgccagatc ctcggctttg gcgagtacgg ctttcccgag tcgcattcgg
     2941 ccagcttcgc actgctggtg tacgtctcgg catggctcaa gtgtcaccac ccggcggcgt
     3001 tctgcgccgc gctgctcaac agccagccga tgggctttta cgcgccgtcg cagctcgtgc
     3061 aggatgcgcg gcgccacggt gtcgaagtgc gggcgccgga cgtttgcgtg agcgcctggg
     3121 actgcgcgct ggagggcggc gcgctgcgcc tggggctgcg cctggtcggc ggactggcgg
     3181 aggtggcggc agcgaaaatc gtcgccgcac gggcgcaggg cgcgttcgcc tcggtcgagg
     3241 atctcgcgcg ccgcgccgcg ctcaaccggc gcgacctcag gtgcctggcg gcgggtggcg
     3301 cgctcgccgg tctcgccggg catcggcgcc aggcacactg gaccgcggcg gggatcgtgc
     3361 aggccgcggg cgtgctgcgc gaagcgccca ttgacgagcc gtcgctcgca ctgccgccac
     3421 ccagtgaagg cgaggacatc gtcgctgact acgcgagcct gggcttgagc ctcggccggc
     3481 atccgctggc gctgctgcgt ccgcgcttga gccgcctcgg cctgctgccg gcggcggcgg
     3541 tccatgcccg tgcgcatggc agcacggcgc gcgcagcggg gctggtgact tgccgccagc
     3601 ggccggacac ggcgagcggc gtgattttcg tcaccctgga agacgagacc ggctgcgtca
     3661 atgtggtggt atggcgcgat ctcatcgagc gccagcgccg cgcgttgctg ggggcgcggt
     3721 tgctcgcggt cgagggccgc atcgagcgcg atggcgaagt cgtgcatctg atcgccgggc
     3781 ggctgagcga tcgcagcgcg ctgctggggc agttgctcgc gccgtcgcgc gacttccgtt
     3841 gagtccgcta agcccctgac cgcctttgac attccgctac gtctggcttc cgcgcaaccc
     3901 ttataattct gcgccaatgt caaagcccaa tcccaccgcg atcatcgcca aggacctggc
     3961 ggacctgata cccgggttct tcacgaaccg aaacaaggag ctcgagctgc tgcgcggggc
     4021 gctcgccggc gcagacttcg agcagctccg acacctcggg catcgcatga agggggttgg
     4081 cgcgtcctac ggattcacca cgatctcgga gatcggcaag cgcatcgaag acggggcgcg
     4141 cagctcgaac cgcggcgtca tcgacgcttg catcggcgag tacgcggact acctggcaac
     4201 ggtgcaggtt gtctatgagt aggtcgagcg ccgatcgccg gcgcgcgcct cgttcagcgc
     4261 cggcgccgtt ccgcgttctg gtggtggacg atgacgtcga catggcaagg ttcctcggcc
     4321 gcttgctcgc ccaggagggc atgaaggtgc agaccgcgct gagcggggaa accgggctcg
     4381 ccagcatcgc ggaatcgccg ccggacctgg tgctgctcga tgtcatgatg cccggcacca
     4441 acggcttcga cgtttgccgg cggctcaaga gcgacgaact gaccgcgctg atccccgtcg
     4501 tgctcgttac gtcgctggaa gacagcgaga gccgggtgcg cggcatcgag gcgggcgccg
     4561 acgacttcct gtccaagccg gtgaagcgcg aggagctgat cgcgcgcgtg aagacgctgc
     4621 gccggctgca cgagacgcgc aaggagctgg agagccggcg cctggccgcc gaggtgcagc
     4681 gcaaggaagc gatccgcaag gccttttcgc gctacatttc gccgcggctg gcggaccgta
     4741 tcgccgaggc ggccggcgag cagggcgacc cgttccaggg ccgtgcccaa cgtacgaatc
     4801 tggtcgccct gtttgccgac ttgcggggtt tcacgcgcct gacggagaag aacgaggtga
     4861 gcgcggtggt cggcatgctg aacgagtact tcgcgatcct gaccgaggcg gcctaccagc
     4921 atgacggcac catcttcaac atggcgggcg acagcctgct ggtcgggttc aatgtgccgt
     4981 tcccgcaggc cgatgccgcc gagcgcgcgt ggcacacggc gctcgagatg gtgggccggt
     5041 tcgcgccggt ggcggccggc tggcacagca cgctcgggct ggagctcggg gtcggcgtcg
     5101 gcatctgcgc cggcgaggcc atcatcggca acgtcggctc cgagcactac gcgagctaca
     5161 ccatcatcgg caacccggta aacaccgcgg cacgcctcat gcagatggcc gctgcgggag
     5221 aggtcctggt ttgcggcccg gtattcgagc gcatccggca cctgctctcc ggccatcgcg
     5281 tggacgggcg cggcgatgtg gcgctgcgcg ggcgctcgca acccattccg gtgttcgcgg
     5341 tgcgtgcggt ggcggagcgc acggcatgag agtgctgatc atcgacgacg atcccgacct
     5401 gcggaacctg ctggcgcact acatccgcca gcaatggccg gggggagaag tcgaggagta
     5461 cgacccgctg gagcgcgaca agccggacgc gcgcttcgcg ctcggcgcgt acgacgtcat
     5521 catcctcgac tacatgctgg gtcgcggcga cggcctgcag tggctgagcg aattcaagaa
     5581 gcgggccgac tgcccgccga tcatcttcct caccggcgcc ggcaacgaga tcatcgccgt
     5641 gcgcgccatg aaggcgggcg cggacgagta tcagcgcaag caggagctca ccaaggagcg
     5701 cctgatcacc gcgatccgcg atgtcgcgca cgacaggatc gagaagaccg tgccgccgga
     5761 agtcgcggcg cgcatggaag gccatatcct cggcgcgcgg atccggatcc cggggatcaa
     5821 ggtgctgcgc ctgatcggcg agggcggcat gtcccgtgtg tacctggcct cgcgcgagtc
     5881 ggacgacgag ccgctggtgg tgaagatcct gcgcagcgag gtaacccagg acaagcgcgc
     5941 gctggcgcgc ttcatcgagg agtacaacct cgtcgagcgc atcagcacgc gccacgtggc
     6001 gcacatccat ggtcacggcg tatccgagga caacgcttac ctggtgatgg agtttttcga
     6061 cggtggcgac ctcaacaagc gtctggccgg gaaggccctg cctgcggagg aggcgctgcg
     6121 ggtctttcgc gagctgatgt tcgcgctggg cgacatccac gagcagggca tcctgcatcg
     6181 cgacctgaag ccgcagaacc tgatgtttcg cgccgacggc agcctggcga tcgtcgattt
     6241 cggcatcgcc aagcacattg actccatcga ccataccaac aagggcgaaa ttctcggtac
     6301 gccgcgctac atgagcccgg agcaggtgca gggccgggcg ctcgacctgc gcaccgacat
     6361 ctacagcgcg ggtgtgctcc tgtaccagat gatgctggga tcgcacctgt tcgaaggcga
     6421 aaccgcggtg gaagtggcgc tgcatcatct gaacacgccg ccgcccggcc ttccagagcg
     6481 cctgcagcgc tatcagcgcc tgttcgacaa gcttctggag aaggaccgcg acgcgcgctt
     6541 tcgcaatgcc gatgaggtca tcggctacct gtcgcgcaag ttctaccagg gccagtagcc
     6601 cgacgcccgc gcgtcgatgc gcaagaagat tccggtggag gaattgcagc tcggcatgtt
     6661 tgtcggcgag ctggaccggc catggatcgg cacgccgttc ctgtaccagg gcttcgtggt
     6721 acgcgaaccg caggagctcg aagcgctcag aaagtactgt cgcgaggtgt acatcgacac
     6781 ggaaaaggct gaccgttcgg tggcgcggcc ggcctcgcgg gccgcgattc ccgggctgcc
     6841 ggggaccggg cgcatcgagc accgggagac cgtggcagtg gaggtcgagt ggcccagggc
     6901 ccgggatgcc ctggagggcg ccggcacggc gctgaccgac gtgttcgaat cggtgcgcgt
     6961 gcgcaagctg ctcgacgcaa cccaggcgcg ttcggcggtg ggcaacatga cgcaaagcat
     7021 gctgcgcaat cccgacgcgc tgatgctatt cgccgcgatg cggcatcgcg gcggctatca
     7081 gctcgagcgc gccatggacg tatcggtcta catgatcgcg ttcgcccgct ttctcggcat
     7141 ggacgaggag gacatcgagc gcgcgggcca cgtgggactg ctgcaggaca tcggcatgct
     7201 ggacctgccg caggagatgc tgcagaaggt gacgcggctg gagccggcgg agttcaagat
     7261 catccgcggc cacgtggcgc gcggccggga ggtgctcgcc gggaccagcg gcctgccggc
     7321 cgacgtggcg cagatcgcag cgctgcatca cgagcgctac gacggctcgg gctacccggg
     7381 cggtctcaag ggcgatgccc tcggcgtgat cggcgccatg gcgggcattg tcgacacttt
     7441 cgacgcgctg acggtgaagc ggccgtatgc cgaggcgatg tccccgtcga acgcgctcaa
     7501 cctgctgttc cggatgcgcg gccgcagctt ccacccgcag ctcgtcgagc agttcatccg
     7561 ctgcgtcggc tactttcccg tcggcagcat cgtggagctg aatagcggcg aagttggcgt
     7621 ggtgatcgcg cagaatgccg agcagcggct gcagcccaag gtcatggtgg tgcgcgatgc
     7681 cgggggccag ccattgcggc cgcagaagtt cctcaatctg gccaggctgc ccaaggcctc
     7741 cggcgccgag ccctaccgca ttcgccgcac cctggaattc ggtcgcgccg ggatatcggt
     7801 cgacgaggtg ctcgtcggct agcgcgggct aaggcagcgt gcgccagatc gcgcgcagct
     7861 gctcgggcag gtccttcggc gagaagggct ttgacagcac gccggcggcg cccatctccc
     7921 gcagctcgtc gagctcgcgc tgcgacgcct tggcggtgat gaacacgacc ggcaggtcgc
     7981 gcaagccgtc ggtttcccgc atgcgcttga acagcgtcgg cccgtccatc accggcatca
     8041 tccagtcgag cagcacgagg tcgggcttga aggcgaggat gccctcgagc gcgacggtgg
     8101 gatcgccgat gatcgacacc tccatcttgc ccacgcgctc cagcgccagg cgtgcgacgc
     8161 gctggatgtc ctcgtcgtcc tcgacgtagc agattcgcag cagcgggcgg tcaggcattc
     8221 agtcctcttt tcaggttgag accgcatcgg ccatcaccgg cagctcgaaa tggaacgtgg
     8281 tcccgccgcc tgggcggtcc tcgaagccga tgcggccgtc catcatctcg atcaggcgct
     8341 tgcagatcgc cagacccagg ccggtgccgc ccttgtggcg cgtggtggtc gagtcggcct
     8401 gggcaaaacg cgtgaagatg cgcgagcgga aatcctccgg gatgccgggg ccgcgatcgc
     8461 tgatcgccac gcgcaccatg ccctcggagc aacgcgtgct gacctcgacc gcggaaccct
     8521 cgggcgagaa cttggcggcg ttcgagagca gattggtcat cacctgcatc agccgcttgc
     8581 ggtccacgtg cacctgtaca tcgggcaact cgccatgcac ctcgagcgcg acgcgaaagc
     8641 ggtctgcgaa ggacttgttg aggaccagcg actcctgcac cagctcgtcc aggcgcatgc
     8701 gctcgggctg cagcgtgaac ttgccggact cgatcttgtc gatgtcgagc aggtcgttga
     8761 tcaggtcgag cagccgctgc gcgttgcgtt cggcaaccga tgacagctcg gcgatctcgc
     8821 cgctcgcgtc gcccacgacg ccgctattca ccagctgcag cgagccgatg atggaggtca
     8881 gcggcgtgcg cagctcgtgc gacagcgtcg aggtgaattc cttcttcagg cgctcggcct
     8941 cgagctgctg cgtgatgtcc tggcactggg cgatgatcga caccagatcg ccgctttcgc
     9001 tgaccagcgg cgtgatcgtg aattcgcaca tgatctcgag gccgtcgcgg cgcaggcagc
     9061 gcacgcgcag gcgggtcacc ggctcccggc gcgcgacgaa tccggcccac cattgcggcg
     9121 tgagcgtggc ctcgtcggcc gggaacagcg tgttcaacgc gctgcggccg atcagctcga
     9181 gcgtcgagta gccgagcatg tgctcggccg cggggttcat ctccaggatc gtggagttgg
     9241 cgtccatttc gatcaccgag atcggcgcac gctcggcgaa cagcgtcagc ttgcgcccgg
     9301 aggcctcgac ctcctggcgt gcctccagca ggtcctcgat ctgctgctcg agctgcttgt
     9361 tgaggcgcgt atggtcctcg gtcaggcggt cgtaggcgtg ccgcaggcgg tagccggaga
     9421 ggaatacccg gttcagccgg taggcgatac cgacgttgat cagatagaag atcggcacca
     9481 gcacggcgat ctcggtgaag agccggttgc cgaaagttcc cagcatgtag gtgaacggca
     9541 gcacgatggc gccggcatac atggcgaaca tcggccacga cgcggcatag gcggggatcc
     9601 cggcggaggt gacgccggcg agcaggaaag cgaggaacac ctgctgctcg tcggggtgcg
     9661 agggaaagaa tacggcgccg gcgaagcccc aggtcacgcc gctggcgagc gcgctgatcg
     9721 ccatccagcg catccactgg tcggcctcca ccagcttgtt gccgctggca cggtagccgt
     9781 agtagaggac ggcgcgcgct gcggtgacga gcaggacgag gccccaccag aacagcacca
     9841 ggtcgcgggt gcgctggtcc cagagctcgt aggtggcgat accgccgagc agcaggtggg
     9901 cgagcatgcc gagcggcatc tggccgtaga gctgctcgac gcggtccgca agtacgcgct
     9961 gcgggtcggc gtggcccggc gcatcttcgg cgcgagcgga agcctcgcgc gtgggcgcca
    10021 gagtgacggg tgctgcgctt ccggaagtca tcgttgggcg ggactcgccc cccgattata
    10081 tcggctgccg cgaagtgccg aggccgcggc tacagcccgc gaccgtttcg aatcacgccg
    10141 accgcgacgc cttcgatggt gaggggatca cggcgcaggt ccacgtcgat cggcgtgaaa
    10201 tccggattct ccggcagcag ctgcaccgcg tgcccacggc gccggtagcg tttcacggtg
    10261 acctcgtcgg cgagccgggc aaccaccacc tggccgctgc gcgcttcctg cgtacggtgc
    10321 accgcgagga gatcgccctc gagaatgccg gcgtcgcgca tcgacatgcc gcgcacgcgc
    10381 aacaggtagt cggcgcgcgg cgtgaacagg ttgggatcga cctgatagcg accctgcagg
    10441 tgctcctccg ccaggatcgg actgcccgcg gcgacgcggc ccaccacagg cagctccatc
    10501 aggcggccat ggcgcggcgc ggatttttcc tttacgcgca ggccgcgcga cgcgccctgc
    10561 aggatctcca gcgcgccctt gcgctccagc gcgcgcaggt gctgctcggc ggcgttcacc
    10621 gagcgaaagc ccatcttctc cgcgatttcc gcccgcgtcg gcgggaagcc cgaggattcg
    10681 gtgagctcgc ggatcagctt cagaatctcg ccctgtcgtt cggtcagcgg ttcgatggcg
    10741 cccatgcggt tccccaagat actgtgtctc cttacagtga tggaagtata tccagtatcc
    10801 ggcggcgcgc aagtagaatg gcccatggcg gcaatcgctg tgcacggggg tgcggggcgc
    10861 tggatggcct ctgatcgcac gctggccgtc gccggtgtgg cgcgcgccgc ggccgtgggc
    10921 tggcacgtgc tggccgcagg gggcagtgcc ttcgatgccg ttgtcgaggc ggtcgcgagc
    10981 cttgaggacg acccgatttt caacgccggc actggctcgg tcctgaacgc acgtgacgac
    11041 gtggaaatgg acgccagcgt gatggcgggc gacacgcttg ccgccggcgg cgtcgcctgc
    11101 atccagaggg tgcgcaatcc cgtgctggtg gcaaagcgcg tgcttcaggt gagcgactgc
    11161 gtcctgctgg cagggcccgc ggcgaccgcg ttcgcgcggg agcagggctt cgaggactac
    11221 gatccgggcc ggccgcgcgc cgcgcacgcc ggccggcgcg gcggcaccgt cggcgcggtg
    11281 gccctggacg atgctgggcg cctggccgcg gcgacgtcga ccggcgggcg cgaaggcaag
    11341 ccgccggggc gcgtgggcga ctcgccagtc ccgggtgccg gcaattacgc ggacgcggcc
    11401 gccgcagtgt cggcgaccgg gtggggcgag cagatgctgc gctacgtcac ggccaaggat
    11461 gtcgcgacga ggatcgcgcg cggcgcgctg cccgagccag cgctgcgtga agcgctcgac
    11521 gatatggcgc gccgcttcgg cgagccgatc ggcgccatcg cgctcgatgc gcgcggccgc
    11581 ctgggcgtcg tgcacggcac agaggcgatg ccgcacgcct ggcggcgcag cggcgaacct
    11641 gaggttaccg cccgtttcgc cgccggtaga atccccgcat gatcatccgc tcgctgctcg
    11701 acaccgacct gtacaagttc accatgatgc aggtggtgct gcatcacttt ccgggcgcgc
    11761 aggtcgagta ccgcttcacc tgccgcacgc cgggcgtgaa tctggtgccg cacctcgagg
    11821 agatcggcgc ggagatcgcg gacctgtgcc ggctgcgttt tcgcgatgag gagctcgcct
    11881 acctgcgcgg gctgcgcttc atgaagtccg acttcgtcga ttttctcggg ctgttccagt
    11941 tcaaccagaa atacatccgc ctcgcgccgc tcggcaagac gggcgaggtc gacatcacca
    12001 tccgggggcc gtggctgcac accatcctgt acgaaattcc ggtgctggcc atcgtgtcgg
    12061 aggtgtactt ccgccgcacg cagcccgggg cggaccttgc ggagggccgc aagcgcctgg
    12121 cgcagaagat cgcgctggta cgcaaggtcg agcccgagct cgagttccgg atctcggact
    12181 tcggcacgcg ccggcgcttt tcgctcgcct ggcacgagga agtggtccag acgctgaaaa
    12241 gcgaggtgcc gcagtatttc gcggggacct ccaacgtgct gctggcgatg cagcaccgcg
    12301 tgacgcccct gggcaccctg gcgcacgagt acatgcaggc ctgccaggcg ctggggccgc
    12361 ggctgcgcga cgcgcaggtg ttcgcgctcg acaagtgggc gcaggagtac cgcggcgacc
    12421 tcggcatcgc gctctcggac acctacggca cggacgcctt cctgcgcgac ttcgacctgt
    12481 atttctgcaa gctgttcgat ggcgcgcgcc acgactcggg cgacccgttc gcctggggcg
    12541 agaagatgat cgcgcactac cagaagaacc gcgtcgaccc gcacaccaag acgctgatct
    12601 tctccgacca gctgtcgttt ccgctcgcga tcgagatcgc gcggcgcttc cacggccggg
    12661 cgcgcaccgc gttcggcatc ggcaccaatc tcaccaacga cgtgggcttc gagccgatca
    12721 acatcgtcat caagatgacc gggtgcaacg gccagccggt ggccaagctt tccgacgccc
    12781 cgggcaagac ggtgagcacc gatccgggct acctcgcgta cctgcgccgg gtgtacgggc
    12841 tggagccagc gccccgttga cgggccaggg ccgcaccgcg gtcgccgtgc tggcgaccgt
    12901 gttcctgttc aacctgctgg gccgtggcag cggcgatacg tatgccgtgt tcctcctgcc
    12961 gctggagcgc gagttcggct gggcacgctc ccagctcacc agcgtgtact cgatgtactt
    13021 gctggtgggc gcgttcatgg cgccgcttgc cggaacgctg ttcgaccgct tcggccctcg
    13081 cgtcacctac agcggcgggc tgctgtgcct gtcggtggcg ttcttcctcg cgtccacgct
    13141 cggcaatctg tgggagttct acctgttcat cggcgtgacc atcggcgtgg gcgtgggcct
    13201 ggtcggcatg gtgccggcgt cggcgctgct cacgcgctgg tttcgcgcgc gcctgtcgag
    13261 cgccatcggc atcgcgttct cggccaccgg caccggcgtg ctgctgttcg tgcctttctc
    13321 gcaatggctg atcgggcagc tgggctggcg cggcgcctac caggtactcg ccggcctggt
    13381 cttcctggcg gtgccgttcg cgctgttcgt cgtgccctgg cgcctgtacg tggcgggact
    13441 gcaggacagc gtaagtggac tgcgcgcgcg cgccggcggc gagggcgtga cggtgcgcgc
    13501 ggccgtgcgc acgcgcacct tttgggcgct ggtgcgggtg ttcttcttca ccgccaccgc
    13561 catgttcatc gtgctggtgc aggcggtggt ctacttcatc gatctcggct tctcgcccat
    13621 cacggcggcc accgcgttcg gcgtcgtggg catgctctcg gtcgtctcgg tgtcgacaag
    13681 cggcctcgcc gccgagcgct tcggctaccg caacacggta agcgcgagct tcgccggcac
    13741 cgcgctgggc gtggggctgc tcgccgtgat gtcggcatgg ccgtcgggca cgctgcttgc
    13801 gatcttcgtc ggggtgttcg ggctgtgcat gggcgtacgc ggcccgatcg tctcgtccat
    13861 cgccacgcgc cgcttcgccg gccccaaggt ggccaccatc tacggcctga tctatgccgc
    13921 caacgccgtc ggcgccgcgt tcggctcgct gatgggtggc gtgctgcacg acctgagcgg
    13981 cggctaccgc gccggcttcg cgctttcggt ctgcgccgtg ggactggcgg tggcgccgtt
    14041 ctggaccgtg aaggagctca gggagttccg ctagcgatgg cgcgcatgcg ctcgaggcgc
    14101 tcgcgcagcg tgcagccgcg caggtcggcg gcgccgcgct cggtgacgat gacgccggct
    14161 tcgctgcgcg gcgtggccac cgggccctgc agcgtttcga cgatgcgggc ggcgggcaac
    14221 agcatcagcg agacgccgcc cggcgattga ttggcggcgc gcacgaaatc gagcgcgccg
    14281 ccgactgcgc cgaggtagct gcccttggcc acctcggcgt tcacctggcc cgtggtgtcc
    14341 acctcgatgg cggagttgat cgccacgaag cgctcgatgc cgccgaggac tcgcgcgttg
    14401 tgcgtgtact cgatcgagcg caggcgcagc ttcgggttct cgtgcgcaaa atcgaacagc
    14461 cggcgtgtgc ccatcaacgt gccgccctgc acgccggcga ccacgccctt ttgcatcagc
    14521 gcggcaacgc cgtcgccgac cgagccggag tggatgcgca ggtcttttcg atcgccgagg
    14581 agctgcagca cggcgtcggg cagggcgcca agtccggtct cgagggtggc gccgtcgggg
    14641 atgaactcga gtgcgttgcg ggcgatggcg agctcctggg ggccgggcgc gccgtagggc
    14701 agggtgacgg gctcgcgcga ggtctcgagc atggcggcga aatccgactt cttcagtaac
    14761 cgctcgctga aggtccaggg cacgcgctcg ttcacctcgg cgatcacgct gcggcacacc
    14821 tcgagcgccg gcaccaggta ttccgccgac agcccgaggc tgtactcgcc cctcgcgttc
    14881 ggcgggctga cctggacgag cacgacgtcg gcccttatcc gcttctgccg aatgagcgtt
    14941 gccagttgcg agtacgggtg cgggtggatg tcgagcacgc ccgcatccgc gagcttgcgg
    15001 ttgagtcccg agccgcagta ggccgtgagg cgaagatgat ctgcgtgctc cggctttacc
    15061 gttcccgacc agttcacgcc catgaagagc gtcgcgccgg tgtattcggc gcgctgcgcg
    15121 accagcgcct cgacgagcgt ctgcggctcg gcgcaggcct gcccgaagat cactccgtcg
    15181 ccggggcgaa tccaccgcga gagctgcatg cggccgatgc taacatcgcc ccagggaggc
    15241 acgatgctcg acgacaaggc gcaggacctg atcttccgcg aagcgcacac gccgcaaggc
    15301 tttctcgact cgccggtacc ggacgcgaag ctgaaacagg tctacgacct gatgaaatgg
    15361 ggccccacca ccatgaacac gcagccgatg cgcatcgtgt tcgtgcgctc gaaggcggcg
    15421 aaagagaagc ttgcgcccgc gctcagctcg ggcaacctcg acaagacgat gaaggcgcaa
    15481 gtgacggcga tcatcgccta tgactcgcgc ttctacgaga acctgccgaa gacctttccc
    15541 aacaacccga aggcaaagga caacttcgca ggcgaagaga agaaggcctc gaacgaccgc
    15601 gtcgcgctgc ggaacggctc gctgcagggc gcctacttca tcatcgccgc gcgcgcgctc
    15661 ggcctggact gcggcccgat gtccggtttc gacaacgcca aggtggacgc ggcgttcttt
    15721 ccggacgggc gctgcaagtc gaacttcctc atcaacctcg gtgaggccga cccggcgaaa
    15781 gtgttcccgc gcaacccgcg actgagcttc gacgaagcct gccgcatcga gtaggactag
    15841 gcgacgcgca acgtcccgtc gacgcaggtc actgcctccc cgccgatctc gatcgcatcg
    15901 gcggtgacgc gcaccgccac gcgcccgtca cgtcctacct gcatgccctg gcgcgcggtg
    15961 tacgaggcgc cgaactcctt cagcagcccg gtttcgcgca gaaaagcgcc gacggagacg
    16021 ttgccgctgc cgcacaccgg gtcctcggga atgccgtgcg cgggagcgaa agagcgcacg
    16081 tggatcggcg ccgccttgtt cccggtacgc ccgaacaccg tgacgccggt cgcatccagc
    16141 gcatcgctga gctcggcgat ggccgtcatg tccggcgcca agccatcgac cgcgccggcg
    16201 tcgccgaggt cgaccacgac ccacaccggc ccgacatcca cccgtaacgc gcgcgcgttg
    16261 ccgaagagcg gcacggtggc ggcggaaacc ttcggcgcgg gtgcgcgcac gtagatgcgc
    16321 tgctcctcca gcgcgagctc gagaatgccc gcaccgcatt cctgccgcag cgtgccgttg
    16381 ctggccttga cgaaaccgct ttcgagcgcg gcgtgcgcgc ttcccaccgt cggatgcccg
    16441 gcgaacggca gctcccggcg cggcgtgaag atgcgcagcc ggtagtcggc cgcggactgg
    16501 gtcggcctga gcaggaacgt ggtctccgaa agattggtcc acgccgcgat gcgttgcatc
    16561 tgtttggcat cgaggccctc ggcgccgatc acgaccgcca ccggattgcc gaggaacggt
    16621 ctgccggtga acacgtccac ttgcttgaag gggagttgcg tcatcgcttc ctgggcgttg
    16681 cgcccttgct gtcgaattcg tagtgcagca ccagaagatc gcgcactggg ctgtcaaggc
    16741 gcaactttat cgtccattcg ccgagcatct ccagatccag ccgtgccttg tactcgcccg
    16801 gcttcgcgcc gggcctggcg cgggcaggcc ggacgttgtg cgccatgggc atcgtgggca
    16861 tgtccgcagc caccgccatt tccgcgccgg cgagcggccg gccgccgcgg ctgagcgtga
    16921 tgatgcagtc gtagacgaaa tcctcgccgg tcgccttgca gcgcagctcg gcctggggag
    16981 attgcgcgtg cgcaagcagg ggagcggcaa agaagatcag gaaaagcgct gcttgagcca
    17041 ttggacaatc ctttcgtcga gcaggccgcg cggccggtaa atcagcacta cggccaccag
    17101 gccgccgaac acgatcatgc gatagccctg gaaaatccgc gtgctctcca gcacgccgat
    17161 gtccaggagc acgcccaaca gcggcccgaa cgcggtaccg agaccaccga tgagcgcgta
    17221 ggcgaggctg tgcacgccga gcatgatgtc gaagtttcgc ggctcgatgt aggtattgtg
    17281 gtgcgcgtac agcacgcctc ccaacgcggc gagcgcaccc gccgctgcgg cgacggcaag
    17341 cttaacgcgc tgcacgtcga tgcccacgga tgcggcgagc tccggatcct caccgacgcc
    17401 acgcagcgtg gcgccgagct tcgagcgctc gacaacgagc aacagcgcca acaccagcgc
    17461 cagcagcacg ccgacgatgc cgagatagtg cagcggctcg atgccgtgct cgaagatcca
    17521 gcggatgccg cggaagccct gcgtcccgtc ggggccggcg agctcaccgt caaccttccg
    17581 gcgccagtgc agcagctcga ataggatgcg cgccatctcg gcggcggcca gcgtcgccat
    17641 ggcgaaatag agcccccgca gccgcaacgt gggcagcgcg accagcaatt gggcgagggc
    17701 gccgagcccc atggcggcga acgcagcgag cggcaagggc catccgtaga gcgcggtcag
    17761 gataccgcca gcgtacgcgc caacaccgaa gaacgcctgc tggccgaatg acatctcccc
    17821 tgcgatcagc agcaggtacg ccgagagggc gacgaacgag aacagaccga tgttggtcag
    17881 gacgccgatc gcgtagtcat ccatggcact ctcgcggcca gcaggacgaa aagcagcagg
    17941 taggcgacga gatcgcgcag ctgcggcccc aggtaccact gcgtgtgcgc ttcgatgaga
    18001 ccgagggcga gcccgccgat cacggcgccg ggcagggagc caagcccgcc gagcatcatc
    18061 gccagcagac ccttcagggt ggcccacatg ccgagcatcg gcgtgatctg gccgtgggtc
    18121 gcgacgacga gatagccggc gatgccgccc agcgccgagg caagcgcgaa cacgcgcatg
    18181 aagacccacg gtacgttgat gccggcgagc gcggcggcgg ccggctgctc gatcagcgcg
    18241 cgcacggcaa ggccgaagcg ggtgcgatac agcgtccacc agagcagggc acaagccgcg
    18301 agcgccgcgg cgagcatcgc cagcgtgtcg acgcgaagcg ttaacggccc cagcgaaagc
    18361 ggatcgccct cgtacagcat ggggaaggcg tacaaatgcc ggggcatgac gagcgtcgcc
    18421 agctcttcga actgcatcca cagcgcaaag ctcgccacca tggcggcgat ggccgaaccc
    18481 ccgcggtgcg gcgcgaagca caggcgctcc acgtaggcgc cggccagcac cgcgccgccg
    18541 accgagatca gcgcgagcag cagcaggccg gcgccgaaat tcacgtgcgc ccatgtcgcg
    18601 caatacgcgc caaacatgat cgacgggcca aaggacaaat tagcgcggcg cagaacgccg
    18661 aagatcaaag tgaagcccag ggcgagcagg gcgtaggacg caccggcgct caggccgtcg
    18721 agcgtgttct ggaggaaatc ggcgagcacc ggctcgactc cggttcgtga tcagcgctgc
    18781 acggatccca ggtacgccct ctggatcacc tcgtcctcgg cgagcgagcc cgggtcgccg
    18841 ctgaaggtca ctcgtccctg ctccatgagg tagaagtgct gcgccacctt gagcgccatg
    18901 tgcgcgttct gctcgacgag gaagatggtg gtgccgtcgc ggtgcagctc ggcgacgatg
    18961 cggaagatct cctctaccac caggggcgcg aggccgagcg agggttcgtc catcagcagc
    19021 agcttcgggc gcgacatcaa ggcgcgcgcc accgccagca tttgctgctc accacccgag
    19081 agggtgcccg cctgctgtgc cgcgcgctcc ttcagtcgcg ggaagcgagc gaacatgcct
    19141 tccatatcgc tcctgacggt tgccttgtcg cgacggtgat aggcgccagc ctggaggttt
    19201 tccgccactg tcatcgccgg gaacacgagc cggttctccg gcaccagcgc cacgccgcgc
    19261 gcgacgatgg catgcggcgc tcgcccggcg atttcctcgc ccgcaaagcg gatgctgccg
    19321 cgcttcggcc tgaggatgcc cgcgagggtc agcatgagcg tggtcttgcc cgcgccgttg
    19381 gggccgagca ggcaggtgat ggcgccctcg gccacctcca gggatacgcc cctcagcgcc
    19441 tcgatcttgc cgtaggcggt gacgatgtcc tggacgctca ggaagttcac gcggcaccgc
    19501 ctccgaggta ggccttgcgc accgccgcgt cggcctggat ctcggccggc gtgccctcgg
    19561 cgatcttctg gccgaagttg agcaccgcga tgcggtcggt gacgcccatc accagctcca
    19621 tcacgtgctc gaccagcagg atggtgacgc cgctcgcgcg cagcttgagg atgcgctcgc
    19681 gcagctgccc gatttcctcg ctgttcatgc cagcggcagg ctcgtcgagc agcagcagcc
    19741 tgggctttgc cgccatggcg cgcgcgagct ccaggcgccg ctgctcgccg aaggcgaggt
    19801 tggcggcaag ctcgttctgc ttgtgcgcga ggcccgagaa ctcgagcagg cggccgatct
    19861 cctcgcgcac gccggcctgg ccgccgagcc agcgcgaaaa gaacccctgg cgcgcccggt
    19921 cggcggcgga attctgcgcc acccacaggt tctgccagac cgagagctgc ccgaacaggc
    19981 ggatattctg gaacgtgcgc gcaatgccct cgcgcagccg caggtgcgtg ggcagcgcgc
    20041 caatgtcgcg gccgcggaac agcaccgcgc ccgcggtcgg cgcgaacagt ccggcgacca
    20101 cgttcaccgt ggtgctcttg cccgagccgt tggggccgat gaggccgaag atctgcccgt
    20161 ccgcgaccgt gaggtcgagc ccgtccaccg cacgcacgcc gccgaaatgg cgcgacagcg
    20221 agcgcagctc aagcatgacg ccgccggccc aggatgccgc tcgggcgcag gttcatggcg
    20281 agcacgatga gcgcgccgaa caggaggttg cgccagtcgc cgaggaagcg cagcccttcc
    20341 accaggaagc cctgtacgaa gatcaccgcg agcagcgtgc cgaagacgtt gcgcaggccg
    20401 cccacgatcg ggtaggcgat ggcgaaggtg gcgaccagga tgttgaagct tgcatgctcg
    20461 atatgggtgg tgtagtgggc gtacagtccg ccgcccacgc cggcgatggc gccgcccacg
    20521 gtcatggcgc tgaccttcac cgccgtgagg ttgatgccca cggactgcgc cgcgagctcg
    20581 tcctcgccga cggcgcgcag cacgctgccg tatcgcgagc gatcgaacca ccaaagcagc
    20641 gccatggcgg caaccacgaa tacccatatg agcagcatca cgtcccaggt ctcccagccg
    20701 ttctccggaa aaaagcggat ctggcggaag ccctcgccgc ccagcgggcc caccttgagg
    20761 ttgcccttgg cgacctggta gtcgaaattg aagaagaaca ggcgcacgcc ttcgccgaac
    20821 gccagggtcg cgatcaccag catcaggccc ttcacgcgca gggcgggaaa gcccacggcg
    20881 caggcgacgc tggcgccggt cagcgcgctg acccccaatg ccggcaccaa gtgccagccg
    20941 gcgagcaccg tcagcatgcc tgccgtgtag gcgccgacgg cgaagaaccc ggcctgcgcc
    21001 aggctgacct ggcccgtcag gagcacgatg tacgccgaca ggccgagcag cgtgtgcacg
    21061 ccgatcagct gcgccagccc gaagtagaag tccacgctta gtccctgccg ccctttggag
    21121 cgaacaatcc gccgggccgg aacgtgagga acacgaacag cagcagcatc acgtacaggt
    21181 cgcgctcggt gacgccgcgg tagagctgca gcacgttctc gaagccgccg accagcaggc
    21241 cggcgacgat cgcgccgggg atgctgccca ggccgccgat gacggtgacg atcagccctt
    21301 tcaccgtcag cggcgtggtc agcagcggcg agagcgtgcc gaccgacgcg ccgatgaggg
    21361 cgccggccag gccgccgagc aggccggtga cgacgaaagt gagcgcgttg acgcgcacca
    21421 ggcgaatgcc gcagagctgc gccgccaccg gctgctgcga caccgcgcgc gtcgccaggc
    21481 cgagcgaggt acggtacagc agcagcagca gagcgatcat ggcgagcacc gacacgccga
    21541 acacgagcag caggtcgccg cgcatgctga actcgcccgc gcgcagcatg acgtcgtcaa
    21601 acatcgccgg gtagctttgc ggcatgccgg cggtcacgtg cacgatgacc tcgtcgatca
    21661 acagcagcat gccgaccgtg gacatcagcg tggcgagcgg gctggagagc gggatgaagt
    21721 gaaagcaggt caggtacacc acccagccga gcacaccgcc ggcgacgaag gcgccgaaga
    21781 aaaccagcgc gggcggggcc tgcgccatca ccgtgagcac gagcagcccg gcataggcgc
    21841 cgcccaccga ggccgcggcg taggccatgt tgatcttgtg catcacgccg aacaccagcg
    21901 tgaagccgat gccgatcagc gcgtaggtcg cgccgaacag cacgccatcg gacaccgtct
    21961 gcagcaagtc gagcatgggc aaagaaacgg gcgcgggatc accgcgcccg tcggctgtgc
    22021 ctccgtgagg ctagagcggg ttgtgcagga ccttccactc gccctttctt gcttgcacga
    22081 atacgtaggg tttgtccgcc tcgccctcgg cgtcgcgctt gctcgtcccg atcagtccgt
    22141 cggtggttct cagcttcgcg aggccgtcgc ggatcttgcg ccggtcggac tgcacgtcgc
    22201 caggcttgcc cgagatgcct tcggattcca tcacttgctt caggatgaac acgttctccc
    22261 aggccgccat cgagtgcagg tcggccgcac ccttgaactt ggcagtggtc ttggcggcga
    22321 aggtcgcgtc cttgttgatc ggcgcgaacg aggtcggaat gatgattcct tcggcctgct
    22381 tggcgcagcc ctgcagcgtc tccaggctcg aggagctggt caggccgacg agcaacttgg
    22441 gctttacgcc ctggcgctgc atctccttga gagcaccgca agcagagaac ggatgagccg
    22501 agatggcgat cacgtccgca ttcgccttct tcatctcgcg cacctgcgta gcgaaattgg
    22561 tgtcggccag cagccacttg gtgttaccca gaacctcgaa accacttgcc gccgcacggt
    22621 ccttcatgac gacgtaccag gtcgcgcgcg agtgcgcgaa gtcctcttcc accccgccgt
    22681 acacggtctt gagttcgggg tgcttggtct tcagccagta gaacagcgag ttgtacatcg
    22741 tcatctcgtt gggcacgttg cggaacgccc attcggagat cttcgccagg ccgaccttga
    22801 tggcgacgtc ggtgaacacc ggcatctgca gtccggaatc gccgctgtcg ccgacttttt
    22861 tctgcaagat cccgaacagg ggctcggcga cgttggagca ggtcggcccc accgcgacca
    22921 ccgcgtcggt tccggcgatg cgtcggatca ccgagatgcc ttcttccgcg ttgcagcggt
    22981 cgtcgtagaa ctcgatctcg atcttggcct tggagccgtc agcgagcttc acgccgcccg
    23041 ccttgttgac gacgtccgcg gcggcgcgca gggccgcttc gctattgacg ccgaacgagc
    23101 gcaccgggcc tgactgagcg ccgaagcctg cgatcttgat ggtctgggcg tgcgcgacgc
    23161 cggcggccag cgccagcgag actgccagag caagtgtttt cacgggaccc cctcctagga
    23221 gtagatttgc gcagccagtg tagaacgggg aggggccgat ggccaagaag aaacgcgttg
    23281 ttgcggtgcg caacaccgat ccgtgggcgg cgctcaagcc cggtgcggca aaccggcagg
    23341 agctctcgcc gctctcgttc ctgccgcgag cagcggagat ctatccgcaa cgggtcgcgg
    23401 tgatccacgg cgccgcccgt atcacctacg cgcagttctt cgagcgagcg cgtcgcctcg
    23461 cctcggcgct cgcccggggc ggcgtggggc gcggcgacgt cgtgtcggcg atgctgccca
    23521 acgtcccggc gatgctcgac gcgcactacg gcgtgcccgt actcggcgcc gttctgaaca
    23581 cgatcaacac gcgcctcgac gcagataccg tcgcctacat cctggagcac gccggcgcga
    23641 aggtgctgat caccgaccgg gtgttcgccg gggtcgtcgg gccggcgctg aagaaactga
    23701 agaacaagcc tttcgtcgtc gaagtcgacg acccgctgta ctccggtccg ggcgagcggc
    23761 tcggatcgat ggagtacgag aagttcctcg ccggcggcga tgccggcttc gcctggcgcc
    23821 agccgcgctc ggaagacgac ccgatcgcgc tcaactacac ctcgggcacc acgggccgcc
    23881 cgaagggcgt tgtgtatcac caccgcggca cctatctgga agcgctcggc aacatcgtcg
    23941 cgtgggcgat gcccgcgcac ccggtctacc tgtggaccct gccgctgttc cactgcaacg
    24001 gctggtgcta cccgtggtcg gtgacggcga tggccggcac gcacgtctgc ctgcgcgcgg
    24061 tggaccccgc gctcatcttt cccatgatcg tcgagcacgg cgtgacgcac atgtgcggcg
    24121 cgccgacggt gctgacaatg ctgatctcgg cgccgcatga gcagcggcgc cccttcgcgg
    24181 ggccggtgca catcatgacg ggcggctcgc cgccgcccgc caaggtgatc cgggacatgg
    24241 aagagctggg cttcaaggtg ctgcacatct acggcatgac cgagctgcag gggccgtcca
    24301 cgttctgcgc ggagcaggac gcctgggcgg cgctcccgct ggaggcgcgc gcggtggaaa
    24361 tggcgcgtca gggggtgcgc taccaggttg tggagggcca catcgtcggc gacccgcaga
    24421 cttgcgagcc ggtgccgaag gacggccgca cgctgggcga gatcctggtt caaggcaaca
    24481 cggttatgtt gggctatctg aaggacgcaa aagccaccgc cgaggcgttc cacggcggct
    24541 ggatgcatac gggcgacctc gcggtctggc atcccagcag ctatattgag atcaaggacc
    24601 gcaagaagga catcatcatc tcgggcggcg agaatatctc gtcggtcgaa gtcgagatcg
    24661 cactctacaa gcacccggcc acggcgctgg cggcggtggt ggcgcggccc gacgagaagt
    24721 ggggcgagac gccgtgcgcc ttcgtgcagc tcaagcccgg ggcgcaagcc accgaggagg
    24781 agttcattca gtggtgccgc gacaatctcg cgcgctacaa ggtgccgcgc aaggttgtgt
    24841 tcggcaatct gcccaccacg gccacgggca agatccagaa gtacgtgctg cgcgagactg
    24901 cgaggacgct ctagcgccta gcggatcagc gtgtcgtcgc ggtaggaggc ggggtcctcg
    24961 atcggctcga ggtgcgtcat caccgcggca ccaggcagct tcgcccggat cttctcctcg
    25021 atctcgtcgg cgatgcgatg cccctcgcgc acgctcatct cgccgggcac cagaagatgc
    25081 atctcgacca ggcgcaggcg ccccgcgcgc cgcgtgcgca gcgcgtggta gtcgaggccg
    25141 tcggcgcggt agccgtccag gatggcgcgg atgcgctcca aatcgggttc gggcagcgcg
    25201 cgatccatca gccccagcac cgaacggcgc atgacccgcg cgccgaccca caggatattc
    25261 accgcgacgg cgatggcgat cagcggatcg agcgcgagcc agccggtcag caccgtggcg
    25321 ccgacaccgg caacgacgcc gatggacgtc cacacatcgg tgagcaggtg gtgcgcgtcg
    25381 gcttccagcg caatcgaatc atggcgcttg cccgcacgca gcagcaccag cgcggtgccc
    25441 aggttcaggg ccgccgcgag cagcgagatg aagatgccga cgtcaaagcg caggatgggc
    25501 tgcggctcga tcaaccgcgg caccgccgcg gcgacgatcg cgatggccgc aacgacgatc
    25561 aacccgccct cgaccgccga cgagaaatac tctgccttgg tatagccgaa tggatgcttc
    25621 tcgtccggct gccgcgtcgc cacgcgcagc gtatagagcg cgatgagtcc cgcgccgagg
    25681 ttgacgaagg attccagcgc gtccgagagc agtccgagcg agcctgtcag ccatgccgcg
    25741 atacccttca gcgccatggt gacgagggcc gttgccacgg aaagccaggc gtagcgggcg
    25801 agggagacca tcggcccgaa gcctatcagg ggattgcgtc cgggcccgga tggttctagc
    25861 atggcatccg gggttcccca acaaggacag acccatgaca aacaacgcgt tagcggccgc
    25921 tcttatcgca gtacttgcag ttcttcccgg gaacgcggct tgggctcaga ccgaggcctc
    25981 gaagccgggc gggatgaagc tcgagcaggg caaacccgac gccgcggcag cgaccgaaaa
    26041 gccgctgaag accgccgccg cgacgggcag gcgtccgacg cgcatcaacg aggatgcccg
    26101 gcactgcctc gatctgccga ccacggcgga gatcgtcagg tgcgctgagc catatcgcta
    26161 ctaacgcgcc gacgggtcca cgccgggccc tgagtttccg ggctcgtagc acagggcagg
    26221 acccttcagc catggcgctc tagccatgga actcgatccc cgcaccctgg ttgtcgcgag
    26281 cgtgcttgcc gcgctcctgc tgggatcggt ttcgatcgcg tttgcccagg tgcaagggtc
    26341 gagccacatc atcggcgctt ggggcagggc gatgctggtg ctcgcgattg gcctgctcgg
    26401 gctggcattg cgcgacgcga tcccgctctg gctctctgcc gtggtgggca atctgctgat
    26461 cgtcgcggct atcgctctgg cgatgcgcgc gctgcgcact ttcgtcggca gcacgccgcg
    26521 cgacgaaacg agctgggcct tgctggcggc gctgttcctg atgctgctgt atttgacgga
    26581 ggtgcgtccc tcccatatcg gtcgcacgat tgccatttcc gcggcgcttg gcatcatcgc
    26641 ctggcgcgcg gcccgtctcc tgcacttgag ggcgcccgcc aactgcaagc tgtcttcgca
    26701 agtcaccgag tacatcttct ggggcgtggc cgcgctcagc ttcgttcgga tcgtcggaac
    26761 ggtgttgttt ccgccggtcg cgtcgctggc cgccggcccg ttcaatgctt ccgtgttcct
    26821 gttctattcc ggactcatca tcgtctccac gttgagcgtc atgtggatgg agatcgagtc
    26881 gttgcagggc gatctcgtgc gtgctgcgcg ctacgatgcg ctcacgaagg tctacaaccg
    26941 cggcacgttc ctggaagaat tcgagcgcga ggtatcacgc tgcgcgcgcg gcagcccggc
    27001 gttcagcctg gcgatcttcg accttgaccg cttcaagcgc ctgaacgatc tgtacgggca
    27061 cccgaccggc gaccgggtgc tgcaggcctt tgccgacgtc ctgcgcgccg acattcgcag
    27121 gcacgatgtc gtgggtcgct atggggggga ggagttcgcg ctgctcatgc cgaacacccg
    27181 caaggaaacc gcgctgcgcg ttgtcgagcg cgtgcgccgc gacctggacg cgcgcggcat
    27241 caccgtggac ggaacgcgca tcgaagtcac ggtgagcggg ggaatctcca cctacggcgc
    27301 cgacggcgaa gactggggca cgctgctgac cgccgccgat accgccctgt acgaagcaaa
    27361 ggacgccggc cgcaaccggg tcgtcaccgc gggcgctgcg ccggtgcccg cgcgggccgc
    27421 gccctcgccg cccggctagc ggcgcgcgct gccgtcctcg gcaaagcgca ccccgaacag
    27481 gcgctcggcc tcagcccgcg tgtagtagcc gcggctcacg tcgagcgcaa ccttcgacgg
    27541 atcgcggcgc agcggatcgc cgtagccgcc gccgcccggc gtcgacacgc ggatgcaatc
    27601 gccgggcgcc agctggatgt cctgatcctt ggacaggtgc ggtggccggt attggctgcc
    27661 ggactgctcg atcgcgacct tgttgacgcc gccttcggcg ccgccgagcg cgcccagggg
    27721 gccgctacgg ccgtggtcca tcaccatcga ggcgcgcgcc
//
LOCUS       DBNHKE010000217         1889 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_358014, whole genome shotgun sequence.
ACCESSION   DBNHKE010000217 DBNHKE010000000
VERSION     DBNHKE010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_358014"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="AC16O9_14820"
     CDS             complement(<1..527)
                     /locus_tag="AC16O9_14820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6517925.1"
                     /translation="MADTTGTIVQCIGAVTDIEFPREGMPKIYDALVLEDTDEGGLAE
                     KGLTFEVEQQLGDGVVRCIALGSSDGLRRGMKVRATGAPISVPVGEGTLGRIMDVLGR
                     PIDDVGPIKSETRRSIHQIAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFGGA
                     GVGKTVNMLELINNIA"
     gene            complement(539..1402)
                     /gene="atpG"
                     /locus_tag="AC16O9_14825"
     CDS             complement(539..1402)
                     /gene="atpG"
                     /locus_tag="AC16O9_14825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR6517926.1"
                     /translation="MPGTKEIRVKIKSVQNTRKITKAMEMVAASKMRKAQERMRMARP
                     YGEKIRTVAAHISHANPEYRHPFLVERDSVKRVGLIVITTDKGLCGALNTNLLRIILT
                     KYKQWQAEGEEIDVCCIGNKGMGFMQRLGANIVSHAVQIGDRPQMDKLIGTIKVMLDA
                     YTEDRFDRLMIGYTRFINTMKQEPVVEQLLPLSGERMGAPETVWDYLYEPEAKAVLDQ
                     VMTRYIEAIIFQAVAENMASEQSARMVAMKAASDNAETLIDELTLVYNKNRQAGITKE
                     LSEIVGGAAAV"
     gene            complement(1414..>1889)
                     /locus_tag="AC16O9_14830"
     CDS             complement(1414..>1889)
                     /locus_tag="AC16O9_14830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6517927.1"
                     /translation="LFNAGIRPAINAGISVSRVGGAAQTKVMKRKDTGGGVRLALAQY
                     RELAAFAQFASDLDEATRKQLERGRMVTELMKQPQYQPLQVWEMAVILFAVNNGYFDD
                     IDVKKALAAERSMRDYVKDKNAALIERVQDKKDLSAEDEKALKDAITDWKKNGSY"
BASE COUNT          271 a          625 c          594 g          399 t
ORIGIN      
        1 gcgatgttgt tgatgagctc gagcatgttc acggtcttgc cgaccccggc gcccccgaac
       61 aggccgatct tgccgccctt ggcgaacggg cagatgaggt cgatcacctt gatgccggtc
      121 tcgagcagct cgaccgaggg gctgagctcg tcgaacttgg gcgcgatctg gtggatcgag
      181 cggcgcgtct cggacttgat cgggccgacg tcgtcgatcg ggcgcccgag gacgtccatg
      241 atgcggccca gcgtgccttc gccgaccggc acggagatcg gcgcgccggt cgcgcgcacc
      301 ttcatgccgc ggcgcaggcc gtcggaggag ccgagcgcga tgcagcgcac cacgccgtcg
      361 ccgagctgct gctcaacttc gaacgtcagg cccttttcgg ccagaccgcc ctcgtccgta
      421 tcctccagga cgagcgcgtc gtagatcttg ggcatccctt cgcgcggaaa ctcgatgtcg
      481 gtgaccgcgc cgatgcactg gacgatggtg cctgtggtgt ctgccatggt tcgattcctc
      541 aaacggcggc ggcgccgcct acgatttccg aaagctcttt ggtaatgccg gcctggcggt
      601 tcttgttgta gaccagtgtc agctcgtcga tcagcgtctc ggcgttgtcc gaggcggcct
      661 tcatcgccac catgcgcgcc gactgctccg aggccatgtt ctcggccacc gcctggaaga
      721 tgatcgcttc aatgtagcgc gtcatcacct ggtcgagcac cgctttcgcc tcgggctcat
      781 acaggtagtc ccagaccgtt tccggcgcgc ccatgcgctc gcccgagagc ggcagcagct
      841 gctcgaccac cggctcctgc ttcatggtgt tgatgaagcg cgtatagccg atcatcagcc
      901 ggtcgaagcg gtcctcggta taggcgtcga gcatcacctt gatggtgccg atcagcttgt
      961 ccatctgcgg ccggtcgccg atctgcacgg cgtgggagac gatgttggcg cccaggcgct
     1021 gcatgaagcc catgcccttg ttgccgatac agcaaacgtc gatctcctcg ccctcggcct
     1081 gccactgctt gtacttcgtc aggattatgc gcaggaggtt cgtgttcagc gcgccgcaca
     1141 ggcccttgtc ggtcgtgatg acgatgaggc ccacgcgctt cacgctgtcg cgctccacga
     1201 gaaacggatg gcggtactcc ggattggcgt ggctgatgtg cgcggcgacg gtgcggatct
     1261 tctccccgta gggccgggcc atgcgcatgc gctcctgggc cttccgcatc ttcgaggcgg
     1321 cgaccatctc catggccttg gtgatcttcc gagtgttctg cacactcttg atcttgaccc
     1381 gaatttcctt ggtgcccggc atatggcctc ggctcagtac gagccgttct tcttccagtc
     1441 ggtgatcgcg tccttcaacg ccttctcgtc ttcggcggag aggtctttct tgtcctgcac
     1501 ccgctcgatc agcgccgcgt tcttgtcctt cacgtaatcg cgcatcgagc gctcggcggc
     1561 gagcgctttc ttcacgtcga tgtcgtcgaa gtagccgttg ttcacggcga acaggatcac
     1621 cgccatctcc cagacttgca gcggctggta ctgcggctgc ttcatgagct cggtcaccat
     1681 gcggccgcgc tcgagctgct ttcgcgtcgc ctcgtcgagg tcggaggcga actgcgcgaa
     1741 tgccgcgagc tcgcggtact gagcgagcgc taggcgcacg ccgccgccgg tgtccttcct
     1801 cttcatcacc ttggtctgcg ccgcgccgcc gacgcgcgac accgagatgc cggcgttgat
     1861 cgcgggccgg atgccggcgt tgaacaggt
//
LOCUS       DBNHKE010000218         6880 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1204821, whole genome shotgun sequence.
ACCESSION   DBNHKE010000218 DBNHKE010000000
VERSION     DBNHKE010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1204821"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="AC16O9_14835"
     CDS             complement(<1..1186)
                     /locus_tag="AC16O9_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517928.1"
                     /translation="MSTPDALQQARDAFDRRAWAEARERLAGADENSPLGAGDLERLA
                     TASYLMGREADFLQCLERAYQAHQKSDDSPRAARCAFWLGLRLLLRGEAGRATAWLSR
                     ARRLVEGLDCVEQGYLLLPAAEQHLDAGDGGTAHAAAADAAGIGERFADADLVACARH
                     LQGRALIQQARLQEGLALLDEAMLAVTAGECSPIVTGLVYCSVIDACREAYALARARE
                     WTSALAQWSEQQPQMVAFTTACLVHRAEILQARGAWAEAIAEARRACERSKQGVDLRP
                     PAAAFYQEAEIHRLRGEFAAAEAAYRSASESGADPQPGLALLRLAQGDAQAAAASIRR
                     ALGATPDRLRRTRLLAAGVEILLAAGEPEEVRGACVELEEIARNHQTDMLDAMAAQAR
                     GAR"
     gene            complement(1311..1721)
                     /locus_tag="AC16O9_14840"
     CDS             complement(1311..1721)
                     /locus_tag="AC16O9_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517929.1"
                     /translation="MHPGQLVASYKFWDIAPLWAAERLDNELVVARALARGVIVDGLR
                     LQSVDASWVKPSRSLTGYPYVGYAADPAKPPVLLRAEALEHLLAVVRQAAVPSRQILG
                     DEFVLRDDFRDWIRATRQMLPAFWFGDGERRVEA"
     gene            complement(1732..2235)
                     /locus_tag="AC16O9_14845"
     CDS             complement(1732..2235)
                     /locus_tag="AC16O9_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517930.1"
                     /translation="MNSLLVAAALAAGAGGALEVTPARTEAAGYAVTHGLVVSNLVRN
                     CRQYRERLKQDPDAALAAWRQRNAERAAAAEGYLVYASTVIERQEGAAAAEAYNKRTR
                     ALFRRQANNTLNDIFNRAHPQPGICARWIDAIAAGEADLNWESKYVPALDELVEFERR
                     VRAGKAN"
     gene            complement(2232..2786)
                     /gene="slmA"
                     /locus_tag="AC16O9_14850"
     CDS             complement(2232..2786)
                     /gene="slmA"
                     /locus_tag="AC16O9_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR6517931.1"
                     /translation="MPRAKGERKLEILKALAQMLEAPRWEKITTAALAAKLDVSEAAL
                     YRHFASKAQMYEGLIEFIETSVFTLANKITADERDGRKQADRLLEMLLAFAEKNPGMV
                     RVMTGDALVGEHERLQARMNQFFDRFEATLKQALRAAADGDAAAHAAMLLRYAIGSLH
                     QFAKSGFAKKPTEGLAQQRAFLAA"
     gene            complement(2796..3464)
                     /locus_tag="AC16O9_14855"
     CDS             complement(2796..3464)
                     /locus_tag="AC16O9_14855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR6517932.1"
                     /translation="MRLVSLPPSRRVWIFDLDNTLHDAQPHIFPSMHDQINLYLMRRF
                     DLDEAGANAMRRGFWQRYGTTMNGLMRHHNTDPREFLAATHQFPELADMVVHEAAVRH
                     ALARLPGKKLIFSNAPRRYVFDVIRALGVARFFEAVYTIESTRYRGKPALAGFRLLLR
                     RHDLDPRRCALVDDMLENLRTAKRLGMATVWVSREKRNVPYVDLRVSSVTQLPGLVFR
                     YSRQ"
     gene            complement(3469..4359)
                     /gene="argB"
                     /locus_tag="AC16O9_14860"
     CDS             complement(3469..4359)
                     /gene="argB"
                     /locus_tag="AC16O9_14860"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR6517933.1"
                     /translation="MNAPSDPQPDAALKARILAEALPYIQRFHGKTIVVKYGGNAMTD
                     EALKSGFARDVALLKLVGMNPVVVHGGGPQIEQMLAKLGKKGEFVQGMRVTDRETMDI
                     VEMVLGGTVNKEVVELINHAGGKAVGLTGQDGVFIRARKLMLPGKDNGRVDLGQVGEI
                     ESIDPSVISALEQGGFIPVVAPIGTGVDGTTYNINADMVAGKLAEILRAEKLVVLTNT
                     PGVLDKEGKLLTGLTPKKFDDLVAKGVISGGMLPKIGSALDAARNGVKSVHIIDGRVP
                     HALLLEVMTDQGVGTLIKSK"
     gene            complement(4434..4862)
                     /locus_tag="AC16O9_14865"
     CDS             complement(4434..4862)
                     /locus_tag="AC16O9_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6517934.1"
                     /translation="MSNITRYDPFNDLMDDLFKGFFVRPYGYEPREAVAPRLKVDVAE
                     KNGAYLVTAEVPGVKKDDIHVSVDGAQVTLEAEVKQEREAGKDERVLHVERTYGKAMR
                     SFTLPQEIDEAKVEAKYHDGVLELTLPKKAAAARKQIAIQ"
     gene            4970..5530
                     /locus_tag="AC16O9_14870"
     CDS             4970..5530
                     /locus_tag="AC16O9_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6517935.1"
                     /translation="MPSADDFRSQIEALRPYLMRFASLQLRDAAAAEDAVQEALLAAL
                     AGEAGFAGRANLRTWLTGILKHKIVDAIRRQAREPLLPQETEEGLQDFDALFERNGHW
                     AERPAGWGDPDGALEQRQFFRALELCLERLPARTAQAFMMREHLGLETPEICKELDIT
                     PTHCWVMLYRARMTLRECLQQTWFGK"
     gene            5527..5721
                     /locus_tag="AC16O9_14875"
     CDS             5527..5721
                     /locus_tag="AC16O9_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR6517936.1"
                     /translation="MMLTCQETTHLVSQGLDRDLAFGERVALRLHLAVCIGCRRTRRQ
                     MAFLRRAVRELVEIDLKTSA"
     gene            complement(5729..6706)
                     /locus_tag="AC16O9_14880"
     CDS             complement(5729..6706)
                     /locus_tag="AC16O9_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR6517937.1"
                     /translation="MQQEQMVPVTILTGFLGAGKTTLLNRILQEDHGHKIAVIENEFG
                     EVGVDSDLIEKSEEQILEMNNGCICCTVRGDLIRILGELKDKRSSGALKFDRVVIETT
                     GMADPGPVAQTFFTDEDIGNYYLLDSILTVVDAKHAPRQLDEFHEAQEQVGFADRILM
                     SKTDLVTEDDVRKLAERIKRMNPRAPVKHVHFGNAPIAEILDIRGFNLNAILELDPEF
                     LVDNHHEHADEVASFVFRSEKPFDGDKLENFLSGMIQVYGPDLLRYKGVLWMKGKSQR
                     VVFQGVHMMMGGDMGKPWAKGEKKQSTLVFIGKKLPKDLFIAGLEQCLA"
BASE COUNT          967 a         2401 c         2349 g         1163 t
ORIGIN      
        1 cgcgcgcgcc gcgcgcctgc gccgccatgg cgtccagcat gtccgtctgg tggttgcgcg
       61 cgatctcctc gagctccacg caggcgccgc ggacttcctc gggctcgccc gcggcaagca
      121 ggatttccac gccggccgcg agcagccgcg tgcgccgcag cctgtcgggc gtcgcgccga
      181 gcgcgcggcg aatggatgcc gcggccgcct gcgcgtcgcc ctgcgccagg cgtagcagcg
      241 ccagtcccgg ttgcgggtcg gcgccgctct cgctcgcgct gcggtaggcc gcctctgccg
      301 ccgcgaactc gccgcgcagg cggtggatct cggcttcctg gtagaacgcc gccgcgggcg
      361 ggcgcaggtc gacgccctgc ttcgagcgct cgcaggcgcg gcgcgcctcc gcgatcgcct
      421 cggcccaggc gccgcgcgcc tgcaggatct cggctcggtg cacgagacac gccgtggtga
      481 acgccaccat ctgcggctgc tgctcggacc attgcgcgag cgcagaagtc cactcgcgcg
      541 cccgcgcgag ggcgtaggcc tcgcggcagg cgtcgatcac gctgcagtag accagcccgg
      601 tgacgatagg cgagcactcg cccgcggtca ccgcgagcat cgcctcgtcg agcagcgcga
      661 gaccctcctg caggcgcgcc tgctggatca gcgcgcggcc ctgcaggtgg cgcgcgcagg
      721 cgacgagatc ggcatcggcg aagcgctcgc cgatgcccgc ggcatcggcc gcggccgcgt
      781 gcgcggtgcc gccgtcacct gcgtcgaggt gctgctcggc ggccggcagc agcagatagc
      841 cctgctcgac gcagtcgagt ccctcgacca gccgccgcgc gcgcgacagc caggcggtgg
      901 cgcggcccgc ttcgccgcgc agcagcagcc gcaggccgag ccagaacgcg cagcgcgccg
      961 cgcgcgggct gtcgtcgctc ttctggtgcg cctggtacgc gcgctcgagg cactgcagga
     1021 agtcggcctc gcggcccatg aggtacgacg cggtcgcgag gcgctcgagg tccccggcgc
     1081 cgagcggcga gttctcgtcc gcgccggcga ggcgctcgcg cgcctcggcc caagcgcgcc
     1141 ggtcgaaggc gtcgcgtgcc tgctgcagcg catcgggcgt gctcacggcc gatagcttaa
     1201 gccggcccgg tccgtggcta cagatccctc atcccgcacg cagatacttg ttccacagca
     1261 gattcgtgag ccgcctcccg gccgccgccg cctcgaggaa gcgccggcgc tcaggcctca
     1321 acgcggcgct ccccgtcgcc gaaccagaac gcgggcagca tctgccgcgt cgcacggatc
     1381 cagtcgcgga aatcgtcgcg cagcacgaac tcgtcgccga ggatctggcg cgagggcacc
     1441 gcggcctgcc gcaccaccgc gagcaggtgc tcgagcgctt cggcgcgcag cagcaccggc
     1501 ggcttcgcgg ggtcggcagc gtagccgacg tagggatagc cggtcagcga tcggctcggc
     1561 ttgacccagc tcgcatcgac gctctgcaac ctgaggccat cgacgatgac gccgcgcgcc
     1621 agcgcacgcg cgaccaccag ctcgttgtcg agccgctccg ctgcccacag cggggcgatg
     1681 tcccagaatt tgtacgaggc gacgagctgc ccggggtgca tggcgcgcct gctagttcgc
     1741 cttccccgcg cgcacgcgcc gctcgaactc caccagctcg tcgagcgccg gcacgtactt
     1801 cgattcccag ttgaggtcgg cctcgcccgc ggcgatggcg tcgatccagc gcgcgcagat
     1861 gccgggctgc ggatgcgcgc ggttgaagat gtcgttgagc gtgttgttgg cctggcggcg
     1921 gaacagcgcc cgggtgcgct tgttgtacgc ctcggcggca gcggcgccct cctggcgctc
     1981 gatcacggtg ctcgcgtaca cgaggtagcc ctcggccgcc gcggcgcgct cggcgtttcg
     2041 ctgtcgccag gcggcgagcg cggcgtccgg gtcctgcttc agccgctcgc ggtactggcg
     2101 gcagttgcgc accaggttgg acaccaccag gccgtgcgtg accgcgtagc ccgcggcctc
     2161 ggtgcgcgcg ggcgtcactt ccagcgcgcc gccggcgccg gcagccaggg cagcggcgac
     2221 gagcaggctg ttcaagcggc gaggaacgcg cgctgctgcg cgaggccttc ggtgggcttc
     2281 ttggcaaagc cgctcttcgc gaactggtgc agcgagccga tggcgtagcg gagcagcatc
     2341 gccgcgtgcg ccgcggcgtc gccgtcggcg gcggcgcgca gcgcctgctt cagcgtcgcc
     2401 tcgaagcggt cgaagaactg gttcatgcgc gcctgcagcc gctcgtgctc gcccaccagc
     2461 gcatcgccgg tcatcacgcg caccatgccc gggttcttct ccgcgaacgc gagcagcatc
     2521 tcgaggagcc ggtcggcctg cttgcgcccg tcgcgctcgt cggcggtgat cttgttggcc
     2581 agcgtgaaca ccgaggtctc gatgaactcg atcaggccct cgtacatctg cgccttggag
     2641 gcgaagtgcc ggtagagcgc ggcttccgag acgtccagct tcgccgcgag cgcggcggtg
     2701 gtgatcttct cccagcgcgg cgcctcgagc atctgggcga gcgccttgag gatttcgagt
     2761 ttccgctcgc ctttggcccg gggcatcggg aaattctact gccgggagta gcgaaagacg
     2821 aggcccggca gctgcgtgac cgaagatacg cgcaggtcga cgtagggcac gttcctcttc
     2881 tcccggctca cccacaccgt cgccatgccg aggcgctttg cggtgcgcaa attctccagc
     2941 atgtcgtcca ccagcgcgca gcgccgggga tcgaggtcgt ggcgcctgag cagcagccgg
     3001 aacccggcga gcgccggctt gccgcggtag cgcgtgctct cgatcgtgta gaccgcctcg
     3061 aaaaagcgcg ccacgccgag cgcgcggatc acgtcgaaca cgtagcggcg cggcgcgttg
     3121 gagaagatga gcttcttgcc cggcaggcgc gccagcgcat ggcgcaccgc ggcctcgtgc
     3181 accaccatgt cggcgagctc ggggaactgg tgcgtggccg cgaggaactc gcgcgggtcg
     3241 gtgttgtggt ggcgcatgag gccgttcatg gtggtgccgt agcgctgcca gaatccgcgg
     3301 cgcatcgcgt tggcacccgc ctcgtccagg tcgaagcgcc gcatcaggta gagattgatc
     3361 tgatcgtgca tcgacggaaa gatgtgcggc tgcgcgtcgt gcagcgtgtt gtcgaggtcg
     3421 aagatccata cccgccgcga gggcggcagg ctgacgaggc gcatcgcgct acttgctctt
     3481 gatgagcgtg ccgacgccct ggtcggtcat cacttcgagc agcagcgcgt gcggcacgcg
     3541 cccgtcgatg atgtgcacgc tcttcacgcc gtttctcgcg gcgtcgagcg ccgagccgat
     3601 cttgggcagc atcccgccgg agatcacgcc tttcgccacc aggtcgtcga acttcttcgg
     3661 cgtgaggccg gtcaggagct ttccctcctt gtcgagcacg cccggggtgt tggtgagcac
     3721 gacgagcttc tcggcgcgca ggatctcggc gagcttgccc gccaccatgt cggcgttgat
     3781 gttgtaggtg gtgccgtcta ccccggtgcc gatcggcgcc accaccggga tgaagccgcc
     3841 ctgctcgagc gccgagatga ccgaggggtc gatggactcg atctcgccca cctggccgag
     3901 gtccacgcgc ccgttgtcct tgcccgggag catgagcttt ctcgcgcgga tgaacacgcc
     3961 gtcctggccg gtgagcccca ccgccttgcc gccggcatgg ttgatgagct ccacgacttc
     4021 cttgttcacc gtaccgccga gcaccatttc cacgatgtcc atggtctcgc ggtcggtaac
     4081 gcgcatgccc tgcacgaact cgcccttctt gccgagcttg gcgagcatct gctcgatttg
     4141 cggcccgccg ccgtgcacca ccaccgggtt catgccaacg agcttcagca gcgccacgtc
     4201 gcgcgcgaag ccggacttca gcgcctcgtc ggtcatggcg ttgccgccgt acttgacgac
     4261 gatggtcttg ccgtggaagc gctggatgta gggcagcgcc tcggcgagga tgcgcgcctt
     4321 gagggcggcg tccggctgcg ggtccgaagg cgcgttcatg tgcggggatt ctacaaacaa
     4381 aaaagggccg gcgcttacgc gccggcccct gtcgactgcg tgcgtgcgca gtgttactgg
     4441 atggcgatct gcttgcgcgc cgccgccgcc ttcttcggca gcgtcagctc gagcacgccg
     4501 tcgtggtact tcgcctcgac cttggcctcg tcgatctcct gcggcagcgt gaagctgcgc
     4561 atcgccttgc cgtaggtgcg ctcgacgtgc agcacgcgct cgtccttccc ggcctcgcgc
     4621 tcctgcttga cttcggcttc cagcgtcact tgcgcgccgt ccacgctgac gtggatgtcg
     4681 tccttcttca cgcccggcac ctcggcagtg accaggtagg cgccgttctt ctcggcgacg
     4741 tccaccttca ggcgcggcgc caccgcctcg cgcggctcat agccgtacgg gcgcacgaag
     4801 aagcccttga aaaggtcgtc catcagatcg ttgaacgggt cataacgggt gatgttgctc
     4861 atagcctgtc tcctttggtc cttgcgggcc ctcaagccct gtgctcccaa ggtaggggcg
     4921 ggggccgggg tttcaagacc ccccgggcct ttagaatcct ccttccgcga tgccgagcgc
     4981 cgacgacttc cgcagccaga tcgaggccct gcgcccctac ctgatgcgct ttgcgagcct
     5041 gcagctgcgc gacgcggcgg ccgccgagga cgcagtgcag gaggcgctgc tcgcggcgct
     5101 cgccggcgag gccgggttcg ccgggcgcgc caacctgcgc acctggctca ccggcatcct
     5161 caagcacaag atcgtggacg ccatccgccg ccaggcgcgc gaacccctgc tcccccagga
     5221 gaccgaggag gggctgcagg acttcgacgc cctgttcgag cgcaacggcc actgggccga
     5281 gcgtcccgcg ggctggggtg atccggacgg cgcgctcgag cagcggcagt tctttcgcgc
     5341 gctcgagctg tgcctcgagc ggctgccggc gcgcaccgcg caggccttca tgatgcgcga
     5401 gcacctcggg ctggagacgc ccgagatctg taaggaactc gacatcaccc cgacccattg
     5461 ctgggtgatg ctgtaccgcg cgcgcatgac cttgcgcgag tgtttgcagc agacctggtt
     5521 cggaaaatga tgctgacctg ccaggaaacc acgcacctcg tgtcccaggg gctggaccgc
     5581 gatctcgcct tcggcgaacg cgtggcgctg cgcctgcacc tcgccgtgtg catcggctgc
     5641 cgccgcacgc ggcggcagat ggcgttcctg cgccgcgcgg tgcgcgagct ggtggagatc
     5701 gacctgaaga cgagcgcctg agcgcggctc aggccaggca ctgctccagg ccggcgatga
     5761 acaggtcctt cggcagcttc ttgccgatga agacgagcgt cgattgcttt ttctcgccct
     5821 tcgcccaggg cttgcccatg tccccgccca tcatcatgtg cacgccctgg aacacgacgc
     5881 gctgggactt tcccttcatc cacagcacgc ccttgtagcg cagcaggtcc gggccgtaca
     5941 cctggatcat gccggagagg aagttctcca gcttgtcgcc gtcgaagggc ttttccgacc
     6001 tgaacacgaa cgaggccacc tcgtcggcgt gctcgtggtg gttgtcgacc aggaactcgg
     6061 ggtcgagctc gaggatcgcg ttgaggttga agccgcggat gtcgaggatc tccgcgatcg
     6121 gcgcgttgcc gaagtgcacg tgctttaccg gcgcgcgcgg gttcatgcgc ttgatgcgct
     6181 cggcgagctt tcgcacgtcg tcctcggtca cgaggtcggt cttcgacatg aggatgcggt
     6241 cggcgaagcc cacctgctcc tgcgcctcgt ggaactcgtc gagctgcctg ggcgcgtgct
     6301 tggcgtccac cacggtgaga atcgagtcga gcaggtagta gttgccgatg tcctcgtcgg
     6361 tgaagaaggt ctgcgccacc gggccggggt cggccatgcc ggtggtctcg atcaccacgc
     6421 ggtcgaactt gagcgcgccg ctcgagcgct tgtccttcag ctcgcccagg atgcggatca
     6481 ggtcgccgcg caccgtgcag cagatgcagc cgttgttcat ctcgaggatc tgctcctcgg
     6541 acttctcgat gagatcgctg tcgacgccga cctcgccgaa ctcgttctcg atcacggcga
     6601 tcttgtgccc gtggtcctcc tggaggatgc ggttgagaag cgtcgtcttg cccgcgccca
     6661 aaaacccggt gaggatcgtc accggcacca tctgttcctg ttgcatggcc taagtataaa
     6721 tggggacaga cctcattatt tcgcagagcg aaaaaatgag gtctgtccct atttcttcag
     6781 cagcaggaga cccttcaggt agtcgccctc ggggaattcg agggccacgg ggtggtcggc
     6841 cgccgcgtgg aagcgctcga tgatctgcgc ctcggcgccc
//
LOCUS       DBNHKE010000219         4872 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1257951, whole genome shotgun sequence.
ACCESSION   DBNHKE010000219 DBNHKE010000000
VERSION     DBNHKE010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1257951"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC16O9_14885"
     CDS             complement(<1..295)
                     /locus_tag="AC16O9_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517938.1"
                     /translation="MINAKLDIGFFEARDARTDVPVQVTLDERGLTLVMPTDSGEPVL
                     LHGERRGQGHYVLRAPNAGIDASLHRFADSTILEGFWRNGRERGFWRLHLPLET"
     gene            521..1576
                     /locus_tag="AC16O9_14890"
     CDS             521..1576
                     /locus_tag="AC16O9_14890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6517939.1"
                     /translation="MIAAVIGRKFPALRDLRVQFAALACVTALPFVAGAAYDIWYAMQ
                     TTSGTILTEARHDVIEVRIGGLALALMVSGLLAAMLLHRMSPTRDSAHAAGAPRNADA
                     LKALLDRRVQSRTRELERSIEELESHAHVVSQRLHAPLSAIQACVTLLESDHRVGLGE
                     EGVGLLGRLRRNAQRIAGLLDVLTEYSRLGRRWLVLQPLDMTEVVKAAIDDLHLSWSE
                     RKRISVARLPRVVADFTLASLVWRHLIDNALKFSARATEREVRIEGAVRHGVAEFRVS
                     DSGVGFDPSQEAKLFTLFERLHGEDEYAGAGTGLAFVKRVVERHGGSVWAEGRPGKGA
                     TFGFALPLGAPIEAPAA"
     gene            1609..4833
                     /gene="icmF"
                     /locus_tag="AC16O9_14895"
     CDS             1609..4833
                     /gene="icmF"
                     /locus_tag="AC16O9_14895"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HIR6517940.1"
                     /translation="MTDLSESRSLAYKAKHKIRFVTAASLFDGHDASINIMRRILQAS
                     GAEVIHLGHNRSVEDVVSAALQEDVQGIAVSSYQGGHIEYFKYMVELLRSRGGGHIQV
                     FGGGGGVIVADEIKELQDAGVARVYSPEDGQKMGLQGMINDMLARCDFDLAQHAPTSL
                     AKNDPRALAQVITVLELGAFKDRAAVLEAAKANPAPVLGITGTGGAGKSSLTDELVRR
                     FRLDQEDRLKIAILSVDPTRKKTGGALLGDRIRMNAIGGANVFMRSLATRDSGAEIAK
                     CLPDAIAACRAAGYGLVIAETSGIGQGNAAIAPLADATLYVMTPEFGAASQLEKIDML
                     DFADFVAINKFDRRGAEDALRDVRKQVQRNREAFKASPDTMPVFGTIAARFNDDGVTA
                     LYHAVADKMKAKGLKLAAGKLPRPASRVSSNVHAVVPPKRARYLAEIAEAVRGYHAHG
                     AAQARIARERQSLREAKRISGLAELDRHLAAKDAALDARAKKLVDAWPQTVKAYSGDE
                     YVVKIRDKEVRTRLTTESLSGTKVPKVALPKFEDAGEILRWQWRENVPGAFPYTAGVF
                     AFKRENEDPTRMFAGEGDAFRTNKRFHLLSRDMPAKRLSTAFDSVTLYGFDPDERPDI
                     YGKVGNSGVSIATLEDMKVLYSGFDLCDPATSVSMTINGPAPTILAMFFNTAIDEQLE
                     KFRKDNGREPTDDEAEKIRAWTLSTVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDI
                     QEYFVHRQVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTFVEAYLARGMHIDDFA
                     PNLSFFFSYGMDPEYAVLGRVARRIWAVAMRERYGANERSQKLKFHSQTSGRSLHAQE
                     IAFNDIRTTLQALISTYDNTNSLHTNAYDEAITTPTEDSVRRAMAIQLIINKEWGLAK
                     NENPNQGSFVIETLTDLVEEAVLKEFEAISARGGVLGAMETGYQRGKIQEESMYYEHK
                     KHDGSYPIVGVNTFRSPHGEAVPARIELIRSTEAEKHGQLKRLREFQQRHAKEAPAML
                     ERLRQAVIRDENVFAVLMDAVRVCSLGQITHALFEVGGQYRRSM"
BASE COUNT          871 a         1623 c         1625 g          753 t
ORIGIN      
        1 ccgtctcgag cggcaggtgc agccgccaga aaccgcgctc gcggccgttg cgccagaatc
       61 cctccaggat cgtgctgtcg gcgaaacggt gcaggcttgc gtcgatgccc gcattcggtg
      121 cgcggagcac gtagtggcct tggccgcggc gctcgccgtg caggagcacc ggctcgcccg
      181 aatcggtggg catgaccagc gtgaggccgc gttcgtccag cgtgacctgc actggcacat
      241 cggtgcgggc gtcgcgcgct tcaaagaagc cgatatcgag cttcgcgttg atcatgcgga
      301 tctcctttcg gagctcgtat ggggaagaag agcgccagcc tagggcttgg cgcgaacgct
      361 gaagagccca gagccggccg agaccgagct ctgtaagtgt ttgaattatg gcactaatag
      421 aacttcgcgg caaggcgctc gatacaacag accgttccgg tgcaagaccg cgcccgccaa
      481 gtgtaatctg cgcttttacg atctcggagg cagagccgcc atgatcgctg cagtcatagg
      541 tcgaaaattt cccgccctgc gcgatttgcg cgtgcaattc gccgcgctgg cctgtgtgac
      601 ggcgctgccg tttgtagcag gggccgcgta tgacatctgg tacgcgatgc agaccacgtc
      661 cgggacgatc ctcaccgagg cgcgccatga cgtgatcgag gtgcgcatcg gcggtctggc
      721 gctcgcgctc atggtctcgg gtctgcttgc cgcaatgctg ctgcaccgca tgtcgccgac
      781 ccgcgattcg gcgcatgccg cgggggcgcc gcggaacgcc gatgcgctga aggccttgct
      841 cgatcgtcgg gttcagtcgc gcacgcgcga actggagcgc tcgatcgagg agctcgagtc
      901 gcatgcccac gtcgtatcgc aacgactgca cgcgccgctg agcgctatcc aagcctgcgt
      961 gacgctgctc gagtccgacc atcgtgtggg gctgggcgag gagggcgtcg gcttgctcgg
     1021 ccgcctgcgc cgcaacgcgc agcgcattgc tgggcttctc gacgtcctga ccgagtattc
     1081 caggctcggg cgcagatggc tcgtgttgca gccgctcgat atgaccgaag tggtcaaggc
     1141 ggccatcgac gacttgcacc tgagctggtc ggaacgaaag cgcatatcgg tcgcgaggct
     1201 tccccgtgtc gtggccgact ttaccctggc gtcgctggtg tggcgccacc tgatcgacaa
     1261 tgcgctcaag ttttcagccc gcgcgacgga acgcgaagtg cgtatcgagg gcgcggtacg
     1321 ccacggcgtc gcggaattcc gggtgagcga cagcggcgtc ggttttgatc cgtcccaaga
     1381 ggcaaagctg ttcacgctgt tcgagcgctt gcacggggaa gacgagtatg cgggcgcggg
     1441 caccgggttg gcgttcgtga agcgcgtggt cgagcgccac ggcggcagcg tgtgggcaga
     1501 agggcggccg ggaaaaggtg caaccttcgg gtttgccctg ccgctcggtg cgccgatcga
     1561 agcgccggct gcttgacgct tacgtaaacg tcaactagcc ttgccgccgt gaccgacctg
     1621 tccgagtcga ggagccttgc ctacaaggcc aagcacaaga ttcgcttcgt caccgcggcg
     1681 agcctgttcg acggccacga cgcctcgatc aacatcatgc gccgcatcct gcaggcctcg
     1741 ggcgcggagg tgatccacct cggccacaac cgctcggtgg aggacgtcgt aagcgccgcg
     1801 ctgcaggagg atgtgcaagg catcgccgtg tcctcctacc agggcgggca catcgagtac
     1861 ttcaagtaca tggtggagct gctccggagc cgcggcggcg gccacatcca ggtgttcggc
     1921 gggggcggcg gcgtgatcgt cgccgacgag atcaaggagc tgcaggacgc cggcgtggcg
     1981 cgcgtctact cgcccgagga cgggcagaag atgggcctgc agggcatgat caacgacatg
     2041 ctcgcgcgct gcgacttcga cctggcgcag catgcgccga cgtcgctcgc gaagaacgac
     2101 ccgcgcgcgc tcgcgcaggt gatcacggtg ctcgagctgg gcgccttcaa ggaccgcgcc
     2161 gcggtgctcg aagccgccaa agccaacccg gcgccggtgc tcggcatcac cggcactggc
     2221 ggcgcgggca agagctcgct gaccgacgag ctggtgcggc gcttccgcct cgaccaggaa
     2281 gaccgcctca agatcgccat cctgtcggtc gacccgacgc gcaagaagac cggcggtgcg
     2341 ctgctcggcg accgcatccg catgaacgcc atcggtggcg cgaacgtctt catgcgctcg
     2401 ctcgccacgc gcgactcggg cgccgagatc gccaaatgcc tgcccgatgc catcgccgcc
     2461 tgccgcgcgg cggggtacgg cctggtgatc gcggaaactt ccggcatcgg ccagggcaac
     2521 gcggccattg cgccgctcgc cgatgcgacg ctgtacgtca tgacgcccga gtttggcgcg
     2581 gcgagtcagc tcgagaagat cgacatgctc gatttcgccg acttcgtggc gatcaacaag
     2641 ttcgaccgcc ggggcgcgga agatgcgctg cgcgacgtga gaaagcaggt gcagcgaaac
     2701 cgcgaggcgt tcaaggcctc gcccgacacg atgcccgtct tcggcaccat cgccgcacgc
     2761 ttcaacgacg acggcgtcac ggcgctgtac cacgccgtgg cggacaagat gaaggccaag
     2821 ggcctgaagc tcgccgcggg caagctcccg cgccccgcaa gccgcgtgtc ctcgaacgtg
     2881 cacgccgtcg tgccgcccaa gcgcgcgcgc tatctcgcgg agatcgccga ggccgtgcgc
     2941 ggctaccacg cgcacggcgc cgcgcaggca cgcatcgcgc gcgagcgcca gagccttcgc
     3001 gaagcaaagc gcatcagcgg gctggccgag ctcgaccggc acctcgccgc gaaggacgcc
     3061 gcgctcgacg cgcgcgcgaa gaagctcgtc gatgcctggc cgcagaccgt caaggcgtac
     3121 tcgggcgacg agtacgtggt gaagatccgg gacaaggaag ttcgcacgcg cctgaccacc
     3181 gagtcgctct cgggcaccaa ggtgcccaag gtcgcgctgc cgaagttcga ggacgcgggg
     3241 gagatcctgc gctggcagtg gcgcgagaac gtgccggggg cgtttcccta caccgcgggc
     3301 gtgttcgcct tcaagcgcga gaacgaggac ccgacgcgca tgttcgcggg cgagggcgac
     3361 gcgttccgca ccaacaagcg ctttcatctg ctgtcgcgcg acatgccggc gaagcgcctt
     3421 tccaccgcct tcgactcggt gacgctgtac ggcttcgacc cggacgagcg ccccgacatc
     3481 tacggcaagg tgggcaactc cggcgtctcc atcgcgacgc tggaggacat gaaggtgctg
     3541 tactcaggct tcgacctgtg cgacccggcg acgtccgttt ccatgaccat caacggcccc
     3601 gcgccgacga tcctggcgat gttcttcaac accgccatcg acgagcagtt ggagaagttc
     3661 cgcaaggaca acgggcgcga gccgacggac gacgaagccg agaagatccg cgcgtggacc
     3721 ctttccaccg tgcgcggcac ggtgcaggcc gacatcctga aggaggacca gggccagaac
     3781 acgtgcatct tctccaccga gttctcgctc aaggtgatgg gcgacatcca ggagtacttc
     3841 gtccaccgcc aggtgcgcaa tttctactcg gtgtcgatct cgggctacca catcgccgag
     3901 gccggcgcga acccgatctc gcagctcgcc ttcacgctcg ccaacggctt caccttcgtc
     3961 gaggcctacc tcgcgcgcgg catgcacatc gacgacttcg cgccgaacct ctcattcttc
     4021 ttctcctacg gcatggaccc cgagtacgca gtgctcgggc gggtggcgcg gcgcatctgg
     4081 gcggtcgcca tgcgcgagcg ctacggtgcc aacgagcgca gccagaagct caagttccac
     4141 tcgcagacct cgggccgcag cctgcacgcg caggagatcg ccttcaacga catccgtacc
     4201 acgctgcagg cgctgatctc gacctacgac aacaccaact cgctgcacac caacgcctac
     4261 gacgaggcga tcaccacgcc caccgaggac tcggtgcgcc gcgccatggc gatccagctc
     4321 atcatcaaca aggaatgggg cctggcgaag aacgagaacc ccaaccaggg atccttcgtc
     4381 atcgagacgc tcaccgacct cgtcgaggag gcggtgctga aggagttcga ggcgatcagc
     4441 gcccgcggcg gcgtgctggg cgcgatggag acgggctacc agcgcggcaa gatccaggaa
     4501 gagtcgatgt actacgagca caagaagcac gacggctcgt acccgatcgt cggcgtcaac
     4561 accttccgca gcccgcacgg cgaggcggtc ccggcaagaa tcgagctgat ccgctccacc
     4621 gaggcagaga agcacgggca gctcaagcgc ctgcgcgagt tccagcagcg ccacgccaag
     4681 gaggcgccgg cgatgctgga gcggctgcgt caggccgtga tccgggacga gaacgtcttc
     4741 gccgtcctga tggacgccgt gcgcgtgtgc tcgctcggcc agatcaccca cgcgctgttc
     4801 gaggtcggag ggcagtaccg caggagcatg tagcgctcag aacgtaaaga gcggccgctc
     4861 cagcttcatg cc
//
LOCUS       DBNHKE010000220         6902 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1311337, whole genome shotgun sequence.
ACCESSION   DBNHKE010000220 DBNHKE010000000
VERSION     DBNHKE010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1311337"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..689)
                     /locus_tag="AC16O9_14900"
     CDS             complement(153..689)
                     /locus_tag="AC16O9_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR6517941.1"
                     /translation="MSKALEEKRLGGEVVFSGRLLKVHRDRVRLPDGSEGAREYIRHP
                     GAVAIVALFEDGRVLLERQFRYPLARTFIEIPAGKLEPGEDHLATGKRELLEETGYVA
                     AEWRKLGLIHNAIGYSDEVIEIWLARGLELRAQQLAEGEFLELFSVPLAEAQAMARDG
                     RITDVKTITGLLWAEGAA"
     gene            complement(689..2188)
                     /gene="nuoN"
                     /locus_tag="AC16O9_14905"
     CDS             complement(689..2188)
                     /gene="nuoN"
                     /locus_tag="AC16O9_14905"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIR6517942.1"
                     /translation="MSPNLIALIDHLPVLPEVILLLGASTLMIVDLLVKDARRGTSFV
                     LAQLVLLACVLATLFVIRGTGTSRLIIFSGMFVADYLANVLKLFGYVAVSAALVYSRH
                     YLHDRGLLRGEFLTLLLFALLGVMVMVSANSFITLYLGLELLSLCLYAMVALNRDSAA
                     SVEAAMKYFVLGALASGLLLYGMSMIYGATGSLLLPEVAARAGALAAGSESARTLLVF
                     GLVFVVAGLAFKLGVVPFHMWIPDVYQGASTAVTLVIGTAPKIAAFAMALRLLVNGLP
                     GLAVDWQQMLIVLAVLSMAIGNITAIAQSNLKRMLAYSTIAHMGFMLLGLLSGVVGGN
                     SLNAVDAYGAAMFYTVVYVLMSLGAFGVLLLVSRAGFECERLEDMKGMNRRDPWVAFL
                     MLLLMFSLAGVPPLAGFYAKFAVISAVVQAGQLWLAVVAVVFSLIGAFYYLRVVKLMY
                     FDDPAGDLAPAGGGGARVLLSVNALALLAIGILPQGLMALCQVAIRWSI"
     gene            complement(2199..3677)
                     /locus_tag="AC16O9_14910"
     CDS             complement(2199..3677)
                     /locus_tag="AC16O9_14910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR6517943.1"
                     /translation="MNAPLLSLAIWVPIGAALLVLASGGDRNAPLQRTIALVGALAGF
                     LVTLPLYAGFDTGTAHMQFVELAPWIGQFNVNYHLGVDGISVLFVILNSFITVLVVIA
                     GWSVIQSRVGQYYASFLMLSGVMNGVFSALDGVLFYVFFEATLIPMFIIIGVWGGPNR
                     VYAAVKFFLYTLAGSLLMLVALLYLYGKSGASFAILDWHKLPLSLAAQTWLFFGFLLA
                     FAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFIRFTLPILPDASRELAW
                     LMIALSLVAVVYIGLVALVQVDLKKLIAYSSIAHMGFVTLGFFVFNAWGVEGALVQMI
                     SHGFVSAALFLCVGVLYDRMHTRMIADYGGVANTMPRFATLMMLFAMANSGLPATSGF
                     VGEFMVILGAMQANFWLAFAAATTLVFGAAYTLWMYKRVMFGAVANERVGALKDLEPR
                     ELVVLGLLAVAVLAMGVYPLPFVEVMHTSVDELLRHVAQPKL"
     gene            complement(3685..5568)
                     /gene="nuoL"
                     /locus_tag="AC16O9_14915"
     CDS             complement(3685..5568)
                     /gene="nuoL"
                     /locus_tag="AC16O9_14915"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR6517944.1"
                     /translation="MKALYLLVPLAPLAGAVVAGFFGRAIGRAGAHWVTILSVLASFL
                     ASCWILVDVLQGNTFNGSVYTWLSSGGVSVEIGFLIDRLTALMMVVVTFVSLMVHIYT
                     IGYMAEDPGYQRFFAYISLFTFSMLMLVMANNFLQLFFGWEAVGLVSYLLIGFWYTRP
                     TAIHANLKAFLVNRVGDFGFLLGIALVLAHFGSLDYAAVFGQAPSSAAPMTLILILLF
                     VGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMAPLYELSPTARS
                     VVLVIGGITALAMALVALTQFDIKRVVAYSTLSQLGYMTVALGASAYSAAIFHLMTHA
                     FFKAVLFLAAGSVIIALHHEQDMRRMGGLKQYMPWTYWTLLLGAISSAGIPGFAGFFS
                     KDAIIEAVRHSATPGALFAWLCVLITVFITAAYTFRLVFMAFHGKPRFDPGHPPHESP
                     AVVTVPLVLLAIPSVLAGYVVGPVVYGDFFGAALPPTAGEFHGLWAFIGHGLTSLPFW
                     LALAGIATAYFLYRVRTDLPKRIAIALGPLYAVVERKYGFDELNAWLFGAGARVLGTG
                     LWKAGDQAVIDGIAVNGSARLVGWFSGVVRLLQSGLLNTYAFLMLFGVLAGVSWVMWR
                     FWQ"
     gene            complement(5565..5876)
                     /gene="nuoK"
                     /locus_tag="AC16O9_14920"
     CDS             complement(5565..5876)
                     /gene="nuoK"
                     /locus_tag="AC16O9_14920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR6517945.1"
                     /translation="MAPLALNHFLVLGALLFAISVVGIFLNRRNVIVLLMAIELMLLA
                     VNLNFVAFSHFLGDIAGQVFVFFILTVAAAESAIGLAILVVLFRNLATINVEDLDSLK
                     G"
     gene            complement(5876..6472)
                     /locus_tag="AC16O9_14925"
     CDS             complement(5876..6472)
                     /locus_tag="AC16O9_14925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR6517946.1"
                     /translation="MFETVVFYVFAAILLLSALRVITARNPVHGALALVLAFFTAAGI
                     WLLLRAEFLAITLVLVYVGAVMVLFLFVVMMLDINLERLREGFWKNLPMAAVVGGIMA
                     FEMVAMLATRHFGASATTRVVPPGYSNTRELGRVLYTQYVYAFEIAAVILLVAIIAAI
                     ALTLRRRKDTKAQDPARQLAARRADSVRLVSMPADRER"
     gene            complement(6492..>6902)
                     /gene="nuoI"
                     /locus_tag="AC16O9_14930"
     CDS             complement(6492..>6902)
                     /gene="nuoI"
                     /locus_tag="AC16O9_14930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR6517947.1"
                     /translation="IVRPKPTLNYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCE
                     AVCPALAIHIESEQRADGTRRTTRYDIDLIKCIYCGFCEEACPVDAIVETRIFEFHGE
                     QRGDLYYTKPMLLAVGERYEESIAKDREADAAFR"
BASE COUNT         1376 a         2337 c         2301 g          888 t
ORIGIN      
        1 ccgctgcaga agatgggaat cctcgagacg cccgcctccg gcggcgcgcc cgtgatgcag
       61 ggcctgaagt gccccgagtg cggcagccgt accctgatcc gcaaggacgg ctgcgactgg
      121 tgcagctcct gcggctacac cgggctctgc ggctaggccg cgccctcggc ccagagcagc
      181 ccggtgatcg tcttcacgtc cgtgatgcgc ccgtcgcgcg ccatggcctg cgcctcggcg
      241 agcggcacgc tgaacagctc caggaactcg ccctcggcga gctgctgcgc gcgcagctcg
      301 aggccgcgcg cgagccagat ctcgatcact tcgtcgctgt agccgatggc gttgtggatc
      361 aggccgagct tgcgccattc ggcggccacg tagccggtct cctcgaggag ctcgcgcttg
      421 ccggtcgcca ggtgatcctc gcccggctcc agcttgcccg cgggaatctc gatgaaggtg
      481 cgcgcgagcg ggtagcggaa ctggcgctcg aggagcacgc ggccgtcctc gaacaaagcg
      541 acgatcgcca ccgcgcccgg gtggcggatg tactcgcgcg cgccctcgct gccgtcgggc
      601 aggcgcacgc ggtcgcggtg caccttgagc aggcggcccg agaacaccac ttcgccgccg
      661 aggcgctttt cctcgagcgc cttcgacact agatcgacca gcggatcgcc acctggcaca
      721 gcgccatcag gccctgcggc aggatgccga tcgcaaggag cgccagcgcg ttcaccgaca
      781 gcagcacgcg cgcgccgccg ccgccggccg gcgccaggtc ccccgccggg tcgtcgaagt
      841 acatcagctt caccacgcgc aggtaataga aggcgccgat cagcgagaac acgaccgcca
      901 ccaccgcgag ccacagctgc ccggcctgca ccaccgccga gatcaccgcg aacttggcgt
      961 agaacccggc gagcggcggc acgccggcga gcgagaacat cagcagcagc atgagaaacg
     1021 cgacccacgg gtcgcgtcgg ttcatgccct tcatgtcctc caggcgctcg cactcgaagc
     1081 cggcgcgcga gacgagcagc agcaccccga aggcgccgag ggacatcagc acgtagacca
     1141 ccgtgtagaa catggccgcg ccgtaggcat cgaccgcgtt caggctattt ccccccacca
     1201 cgcccgagag cagcccgagc agcatgaagc ccatgtgcgc gatggtcgag taggccagca
     1261 tgcgcttcag gttcgattgc gcgatggcgg tgatgttgcc gatcgccatc gacagcacgg
     1321 cgaggacgat cagcatctgc tgccagtcca ccgccagccc gggcaggccg ttcaccagca
     1381 gccgcagcgc catcgcgaag gcggcgatct tcggcgcggt gccgatgacc agcgtcaccg
     1441 cggtgctcgc gccctggtag acgtcgggga tccacatgtg gaacggcacc acgccgagct
     1501 tgaaggcaag gcccgccacc acgaacacca ggccgaacac gagcagcgtg cgcgcgcttt
     1561 ccgagccggc ggcgagcgcc ccggcgcgcg ccgcgacctc ggggagcagc agcgagccgg
     1621 tggcgccgta gatcatcgac atgccgtaga gcagcagccc cgaggcgagc gcgccgagga
     1681 cgaagtactt catcgccgcc tcgacggagg ccgccgagtc gcggttcagc gccaccatgg
     1741 cgtacaggca cagcgacagc agctccaggc cgaggtagag cgtgatgaag ctgttcgcgc
     1801 tcaccatcac catgacgccg agcagcgcga acagcagcag cgtaaggaac tcgccgcgca
     1861 gcaggccgcg atcgtgcagg taatggcgcg agtacaccag ggccgccgac accgccacgt
     1921 agccgaacag cttcagcacg ttggccaggt agtccgccac gaacatgccg ctgaagatga
     1981 tcagccggct ggtgcccgtt ccccggatca cgaacagggt cgcgaggacg cacgccagca
     2041 ggaccagctg cgccagcacg aagctcgtgc cgcgacgggc gtccttcacc agcaggtcca
     2101 cgatcatcag cgtcgacgcc ccgagcagga ggatgacctc gggaagcacc ggcaggtggt
     2161 cgatgagcgc gatcaggttc ggcgacatgg ctagcgcatc acagcttagg ctgcgccacg
     2221 tggcgcagca gttcgtcgac cgaggtgtgc atcacctcga caaaaggcag cgggtatacg
     2281 cccatcgcga gcactgccac ggcgagcagc cccagcacga ccagctcgcg cggctcgagg
     2341 tccttgagcg ccccgacgcg ctcgttggcg acggcgccga acatcacccg cttgtacatc
     2401 cacagggtgt acgcggcgcc gaacacgagt gtcgtcgcgg cggcgaaggc gagccagaaa
     2461 ttggcctgca tcgcccccag gatcaccatg aactcgccga caaagccgct ggtggccggc
     2521 aggccgctgt tggccatcgc gaacagcatc atcagcgtgg cgaagcgcgg catggtgttc
     2581 gccaccccgc cgtagtccgc gatcatgcgc gtgtgcatgc ggtcgtagag cacgccgacg
     2641 cacaggaaca gcgccgccga gacgaaaccg tgcgagatca tctgcaccag cgcgccctcc
     2701 acgccccagg cgttgaagac gaagaagccc agcgtgacga agcccatgtg cgcgatcgac
     2761 gaataggcga tgagcttctt caggtcgacc tgcaccagcg ccaccaggcc gatgtacacc
     2821 acggcgacga gcgagagcgc gatcatcagc cacgccagct cgcgcgaagc gtcgggcagg
     2881 atcggcagcg tgaagcggat gaagccgtag gcgccgagct tcaacatgat cgccgccagc
     2941 accaccgagc cgccggtcgg tgcctcgacg tgggcgtcgg gcagccaggt atgcaccggc
     3001 cacatcggca ccttgacggc gaaggcgagc aggaagccga agaacagcca cgtctgcgcc
     3061 gcgagcgaca acggcagctt gtgccagtcg agaatggcga agctcgcgcc cgacttgccg
     3121 tacaggtaca gcagcgcgac cagcatcagc agcgagccgg cgagcgtgta gaggaagaac
     3181 ttgaccgccg cgtagacccg gttcggcccg ccccagacgc cgatgatgat gaacatcggg
     3241 atcagcgtcg cctcgaagaa cacgtagaac agcacgccat ccagcgccga gaacacgccg
     3301 ttcatcaccc ctgagagcat gaggaacgac gcgtagtact gtccgacgcg gctctggatc
     3361 acgctccagc ccgcgatcac caccagcacg gtgatgaacg agttcaggat cacgaacagc
     3421 accgagatgc cgtccacgcc aaggtggtag ttgacgttga actggccgat ccagggggcg
     3481 agctccacga actgcatgtg cgccgtgccg gtgtcgaaac cggcatacag cggcagcgtg
     3541 accaggaagc cggcaagcgc gcccacgagc gcgatcgttc gctgcagcgg cgcgttgcgg
     3601 tcgccgcccg aggcgagcac gagcagtgcc gcgccgatgg gcacccagat ggcaagcgac
     3661 aggagcggcg cgttcatcgg taggctactg ccagaaccgc cacatgaccc agctgacccc
     3721 ggcgagcacg ccaaacagca tcaggaacgc ataggtgttg agcagcccgc tttgcagcag
     3781 gcgcaccacg cccgagaacc agcccaccag gcgcgccgag ccgttcacgg cgatgccgtc
     3841 gatgacggcc tggtcgcccg ccttccacag cccggtgccc agcacgcgcg ctccggcgcc
     3901 gaacagccag gcgttgagct cgtcgaagcc gtacttgcgc tccaccaccg cgtacagcgg
     3961 gcccagggcg atggcgatgc gcttgggcag atcggtgcgc acacggtaca ggaagtacgc
     4021 cgtggcgatg ccggcgagtg cgagccagaa cgggagcgac gtcaggccgt ggccgatgaa
     4081 ggcccacaag ccgtggaact cgccggcggt aggcggcagc gccgcgccga agaaatcccc
     4141 gtacaccacc ggccccacga cgtagccggc cagcacggac gggatggcga gcagcacgag
     4201 cggtacggtc accaccgcgg gcgactcgtg cgggggatgc ccgggatcga agcgcggctt
     4261 gccgtgaaag gccatgaaca cgagccggaa ggtgtatgcg gcggtgataa agacggtgat
     4321 cagcacgcag agccaggcga acagcgcccc gggcgtcgcc gaatgacgca ccgcctcgat
     4381 gatcgcgtcc ttggagaaga agcccgcgaa ccccggaatg ccggccgacg agatcgcgcc
     4441 caggagcagc gtccagtagg tccacggcat gtactgcttc agcccgccca tgcggcgcat
     4501 gtcctgctcg tggtgcagcg cgatgatcac cgagcccgca gcgaggaaca gcaccgcctt
     4561 gaagaatgcg tgcgtcatga ggtggaagat ggcggccgag tacgccgaag caccgagcgc
     4621 caccgtcatg tagccgagct gcgacagcgt cgagtaggcc acgacgcgct tgatgtcgaa
     4681 ctgcgtgagc gcgaccagcg ccatggcgag cgccgtgatg ccgccgatca ccagcaccac
     4741 cgagcgcgcg gtcggcgaga gctcgtacag cggcgccatg cgcgccacca tgaagatgcc
     4801 cgcggtcacc atggtggcgg cgtggatcag cgccgagatc ggcgtcgggc cttccatcga
     4861 gtcgggcagc cagacatgca gcgggaactg cgcgctcttg cccatggcgc cgacgaacag
     4921 caggatcagt atcagcgtca tcggcgcggc tgacgagggc gcctgcccga acaccgcggc
     4981 gtagtcgagg ctgccaaagt gcgccagcac cagcgcgatg ccgagcagga agccgaagtc
     5041 gccgacgcgg ttgaccagaa acgccttcag gttggcgtgg atcgccgtgg gacgcgtgta
     5101 ccagaagccg atgaggagat aggacaccag ccccaccgcc tcccagccga agaacagctg
     5161 caggaaattg ttcgccatca ccagcatcag catcgagaag gtgaacagcg agatgtacgc
     5221 aaagaacctc tggtagccgg gatcctcggc catgtagccg atggtgtaga tgtgcaccat
     5281 cagcgagacg aaggtcacca ccaccatcat cagcgcggtg agccggtcga tgaggaagcc
     5341 gatctcgacg ctgaccccgc cgctcgagag ccaggtgtag acgctgccgt tgaaggtgtt
     5401 gccctgcagg acgtcgacga ggatccagca cgaggcgagg aacgacgcca gcaccgacag
     5461 gatcgtcacc cagtgcgcgc ccgcgcgccc gatcgcgcgc ccgaagaacc cggccacgac
     5521 cgcgccggcg agcggcgcca gcggaacgag gagatagagc gccttcatcc cttcagcgaa
     5581 tccaagtctt cgacgttgat ggtggcgagg ttcctgaaca gcaccaccag gatcgccagg
     5641 ccgatggccg actccgcagc cgccaccgtg aggatgaaga acacgaacac ctgccccgcg
     5701 atgtcgccga ggaagtgcga gaaggcgacg aagttgaggt tgacggcgag cagcatgagc
     5761 tcgatcgcca tcagcagcac gatgacgttg cggcggttga ggaagatgcc caccacgctg
     5821 atcgcgaaca gcagcgcgcc gagcaccagg aaatgattga gcgcgagcgg cgccatcagc
     5881 gctccctgtc tgcgggcatg ctgaccaggc gcacgctgtc tgcgcgacgt gccgccagct
     5941 ggcgcgccgg gtcctgcgcc ttggtgtcct tgcgacggcg cagcgtcaac gcgatggccg
     6001 cgatgatcgc caccagcagg atcacggcgg cgatctcgaa cgcgtacacg tactgcgtgt
     6061 agagcacgcg gccgagctcc cgggtgttgc tgtagcccgg cggcacgacg cgcgtggtcg
     6121 ccgacgcgcc gaagtggcgg gtcgccagca tggcgaccat ctcgaaggcc atgatgccgc
     6181 cgaccaccgc cgccatcggc aggttcttcc agaacccctc gcgcaggcgc tcgaggttga
     6241 tgtcgagcat catcaccacg aacaggaaca gcaccatcac cgcccccacg tacaccagca
     6301 ccagcgtgat ggcgaggaat tcggcgcgca gcagcagcca gatgccggcc gcggtgaaga
     6361 aggcgagcac cagcgccagc gcgccgtgca ccggattgcg cgcggtgatc acgcgcagcg
     6421 ccgagagcag caagatggcg gcgaagacgt agaagaccac ggtctcgaac atgcgtcagt
     6481 aggcccggtt gtcagcggaa ggcagcgtcg gcctcgcggt ctttcgcgat actctcttca
     6541 tagcgttcgc ccactgccag cagcatgggc ttggtgtagt acaggtcgcc gcgctgctcg
     6601 ccgtggaact cgaagatgcg cgtctcgacg atggcgtcca cggggcaggc ctcctcgcag
     6661 aagccgcagt agatgcactt gatgaggtcg atgtcgtagc gcgtggtgcg gcgcgtgccg
     6721 tcggcgcgct gctccgactc gatgtgaatg gcgagcgccg ggcacaccgc ctcgcacagc
     6781 ttgcacgcga tgcagcgctc ctccccggaa gggtagcggc ggagcgcatg ctcacctcta
     6841 aagcgcggcg aaagcggacc tttttcaaag ggatagttta gtgtcggctt ggggcgaacg
     6901 at
//
LOCUS       DBNHKE010000221         2666 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1363666, whole genome shotgun sequence.
ACCESSION   DBNHKE010000221 DBNHKE010000000
VERSION     DBNHKE010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1363666"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..756
                     /locus_tag="AC16O9_14935"
     CDS             148..756
                     /locus_tag="AC16O9_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517948.1"
                     /translation="MSFSTNELRQLALPLLACLALLGAGSALITWTEARNAAADRALT
                     AARAERSQANERLMRIAEEEREVKEKLEIYQRLKRAGILGEERRLEWADAMARIRTER
                     ELPDLRYRVERQLPLKTVPGKPANVEFYASTMRVVLALLHEGDLLHFLSDLRASGNAY
                     YAVRSCSLARTGQPIASSVLSPRLRAECEIDLITVLDLAAKT"
     gene            753..1190
                     /locus_tag="AC16O9_14940"
     CDS             753..1190
                     /locus_tag="AC16O9_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517949.1"
                     /translation="MRQAPALVLACALLAPGAAAAQEQLGRLFFTPEQREALDARRRA
                     RLPDKPAAVAVSPTTRIDGYVKRSSGKSTLWVDGYAVRDGSHPEGLRVRAGRDPSRVT
                     VRIGEDGRPIEIKVGQTLDRGNGEITDALGGGEVRVERRGAAR"
     gene            1231..>2666
                     /locus_tag="AC16O9_14945"
     CDS             1231..>2666
                     /locus_tag="AC16O9_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517950.1"
                     /translation="MLGLLAVAVMVFAYVLTSRLNAASRFVGVDRETNARVLSQAKQA
                     LIGWMAVNATADNNPGRLPCPEAVNAIGTPSEGISAPLVTPSTPNCATVGRLPWRTLG
                     LSRLVDAASEPLWYAVSPGWALQNSSTLLSINPDSLGALVIDGQPAPNEVVALIIAPG
                     PAMNVQAAAGCNAVAQARAAPAPSMNPANYIECFNAATPAFSTTGPVTSFNDQVVRVT
                     VADLMPALEAAIADRAQREIAPAVRSTGFELDTNTPRRWIGAAPLPSGNPRLYPYAVP
                     FTDPTTPVSSYLGVTGTYQGLMPFASSASAVAFESTPPNVIKTTAAGTLKTVNCYWES
                     ADVYACEGEYEKDADPTLDITLQMTATFTNVAMGLRARNTAALTAEARNNSGDPWITT
                     GMSISKLLEINDGSSAGKPRGSVTAQLDATLPNIAAMGWGNKALYRLRMGRTVVADHP
                     ILSATGRALRFSGGSSAINSGNTVTGATS"
BASE COUNT          424 a          946 c          915 g          381 t
ORIGIN      
        1 gctgcgcgcc gatcccgcct accagatcga gcgcacgcaa ctgccgtttg acgtcacttc
       61 ggagggcacg ctgtccggcg atatcagcac cgccacggaa accggcgagg cgccgcgttt
      121 caccatcgtg ctgacgcgga gaatcctgtg agcttctcga cgaacgagct gcgccagctc
      181 gccctgccct tgttggcgtg cctcgcgctg ctcggcgcgg gctcggcgct catcacctgg
      241 accgaggcca ggaacgccgc cgcggaccgg gcgctcaccg ccgcgcgtgc cgagcgctcg
      301 caggccaacg agcgcctgat gcgcatcgcc gaggaggaac gcgaggtcaa ggagaagctg
      361 gagatctacc agcggctcaa gcgcgccggc atcctcggcg aagagcggcg gctggaatgg
      421 gccgatgcca tggcgcgcat ccgcaccgag cgcgagcttc ccgacctgcg ctaccgcgtc
      481 gagcgccagc tgccgctgaa gaccgtgccc ggcaagccgg cgaacgtcga gttttacgcc
      541 agcaccatgc gcgtggtgct cgcgctgctg cacgagggcg acctgctgca tttcctgagc
      601 gacctgcgcg cctccggcaa cgcctactat gccgtgcgca gctgctcgct cgcgcgcact
      661 ggccagccaa tcgcgagcag cgtgctctcg ccgcggctgc gcgcggagtg cgagatcgac
      721 ctgatcaccg tcctcgacct ggcggcgaag acatgaggca ggcacccgcc ttggtgttgg
      781 cgtgcgcctt gctggcgccg ggagcggcgg ccgcgcagga gcagctgggg cggttgttct
      841 ttaccccgga gcagcgcgaa gcgctcgacg cgcggcgcag ggcgcgcctg ccggacaagc
      901 ccgccgcggt tgcggtatcg ccgacgacgc gcatcgacgg ctacgtcaag cgctcgagcg
      961 gcaagtccac actgtgggtc gacggttatg cggtgcgcga cgggtcccac cccgagggcc
     1021 tgcgcgtgcg cgccgggcgc gacccgagcc gcgtcacggt gcggatcggc gaagacggcc
     1081 gtcccatcga gatcaaggtc ggccagacgc tcgatcgcgg caatggcgag atcaccgacg
     1141 cgctcggcgg cggcgaagtg cgggtcgagc gccgtggggc ggcgcgctag cccacgcgcc
     1201 gcggggcgcc gcgcgcagcg cggcgtggcc ttgctcggcc tgctggctgt cgcggtcatg
     1261 gtcttcgcgt acgtgctgac cagccggctt aacgcggcga gccggttcgt cggcgtcgat
     1321 cgcgagacca acgccagggt cctcagtcag gcgaagcagg cgctgatcgg ctggatggcg
     1381 gtcaacgcga cggccgacaa caacccggga cgcctgccct gccccgaggc ggtcaatgct
     1441 atcggcacgc cgtccgaggg catctccgcc cccttggtaa cgccgtcgac gccgaactgc
     1501 gccaccgtgg gccggctgcc ctggcgcacg ctggggctca gcaggctcgt ggacgccgcc
     1561 tctgagccgc tctggtacgc ggtctcgccc ggatgggcgc tgcagaacag ctcgacgctg
     1621 ctcagcatca acccggacag cctgggcgcc ctggtcatcg acgggcagcc cgcgccgaac
     1681 gaagtcgtgg cgctgatcat tgcgccaggc ccggcgatga acgtgcaggc cgccgccggg
     1741 tgcaacgcgg tcgcacaggc gcgtgccgcg ccggcaccgt ccatgaaccc tgccaactac
     1801 atcgaatgct tcaacgcggc gacgccggcg ttctcgacga ccggaccggt aacgtccttc
     1861 aacgaccagg tggtgcgcgt gaccgtggcg gacctcatgc ctgcgctcga agcggcgatt
     1921 gcggatcgcg cgcagcgcga gatcgcgccg gcggtgcgca gcacggggtt cgagctcgac
     1981 acgaatacac cgcggcgctg gataggcgcg gcccccctgc cttccggcaa tccgcggctc
     2041 tatccctacg cggtgccttt caccgatccg accactccgg tgtcgagcta cctcggcgtc
     2101 accggaacct accagggctt gatgcccttt gcgtcttcgg cgagcgcggt agcgttcgag
     2161 tcgactccgc cgaacgtgat caagacgacc gcggccggca cgctcaagac cgtcaactgc
     2221 tactgggagt cggcggacgt gtatgcctgc gagggtgaat atgaaaaaga cgccgacccg
     2281 accctggaca tcaccctgca gatgacggcc acgttcacga acgtcgccat gggtcttcgc
     2341 gcccgtaata ccgccgcgct caccgcggag gcgcgcaaca acagcggcga tccgtggatt
     2401 acgaccggca tgtccatatc gaagctgctt gagatcaacg acggcagcag cgcgggtaag
     2461 ccgcgcggct cggtcacggc tcagctggac gcgaccctgc ccaacatcgc cgctatgggc
     2521 tggggcaaca aggcgttgta ccgcctgcgc atgggccgca ccgtggtggc cgatcacccc
     2581 attttgagcg cgaccggcag ggccctgcgc ttcagcggcg gctccagcgc gatcaacagc
     2641 ggcaataccg tgaccggcgc gacctc
//
LOCUS       DBNHKE010000222        26547 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_200083, whole genome shotgun sequence.
ACCESSION   DBNHKE010000222 DBNHKE010000000
VERSION     DBNHKE010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 26547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 26547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_200083"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16O9_14950"
     CDS             complement(<1..224)
                     /locus_tag="AC16O9_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6517951.1"
                     /translation="MLETAVGELIESRRLVVASPDTSVLDAARQMRVSHVGAVLVVRG
                     EQLVGIFTERDALNRVMAEGRDPAATRLAEV"
     gene            332..688
                     /locus_tag="AC16O9_14955"
     CDS             332..688
                     /locus_tag="AC16O9_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517952.1"
                     /translation="MLTTGALLLAAVALALGIVLHLRRRRARTDDRMRLYDIMRRRGV
                     SPPPAEDAVGAQDVAAAERRCAACANKEMCDELLRIGQTRGYDRFCPNALYIEWLRSN
                     SLDFELPPPPSAGRPD"
     gene            complement(668..1672)
                     /locus_tag="AC16O9_14960"
     CDS             complement(668..1672)
                     /locus_tag="AC16O9_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517953.1"
                     /translation="MAPRWRVPLLVLGFASLALGIAGGLARLGAALPAPAGAIALHGA
                     LMVSGFLGTVIGLERAVALGRLWPYAAPLASGLGGLCLILGFAAPGFALLALAAALLA
                     AASLAVLARQPSPEMATLAAGAVAWLAGNLALYAGAAAVPWWIAFLALTIGGERLELS
                     RYLRRGAFARGSFVLLAAALLAAPVFPRVLGLVLVLLAAWLFAFDLARVTVRQRGLPR
                     YIAVCLLSGYAWLAFGGALLATLTAYDAALHAIFVGFVFSMVFGHAPVILPAVLRVPL
                     PYHAALYVPLALLHLSLALRVFVSPLAGAWGNAAAVALFIATAATLAVRRARSVRTAC
                     "
     gene            complement(1676..1978)
                     /locus_tag="AC16O9_14965"
     CDS             complement(1676..1978)
                     /locus_tag="AC16O9_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR6517954.1"
                     /translation="MDEKEVMQALRGVEDPEAGMSVVDLGLVYGVQAAPGRVRVQMTM
                     TSPACPAAPYLVDEAMAAIRALAPAGTDVAVELVWDPPWTPERMSAEAQSRFGWTG"
     gene            2059..2373
                     /locus_tag="AC16O9_14970"
     CDS             2059..2373
                     /locus_tag="AC16O9_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517955.1"
                     /translation="MHFMLVILIFLVGAVLWGYFYANPQGVSRGKLLACNIAVLALGA
                     AIAAAAGVLLLEDGLRVKPGEPGLAWFLAIMGAGTAFMIVVASGGLVRNLLLFPLSKR
                     SR"
     gene            2426..4285
                     /locus_tag="AC16O9_14975"
     CDS             2426..4285
                     /locus_tag="AC16O9_14975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6517956.1"
                     /translation="MRLLLVNPALPESFWSFRWALERVLGGKRALNPPLGLATLAALC
                     PPHWQVTIVDENVEPLPLEPHADLIGIGGMGVQAPRQKQLLRYYREAGYRVVAGGSYA
                     SLCPEEYREHADHVIAGEAEYIWPKFCTDLERGVAQALYRETGEVRLEDSPVPRFDLL
                     KLDAYSTASLQLSRGCPFRCEFCDIIVMFGRKPRYKTPERIERELDSLRRLGARKVFF
                     VDDNFIGNKARAKETLRCVAAYQARHGWRMRFGTEASLNLADDPELLALFREAGFEWA
                     FLGLETPDPQTLREAAKSQNTAGDMLGAVRRIYAAGVDILGGFIVGFDRDTPAAFEKQ
                     RRFILDSGIMVAMVGLLTALPKTPLYERLKREGRLLERADHGDNTRARTNIVPKSMSV
                     AQLTAGYQRLYARLLTDGAIAERIRNKLAHFGASARLVRETPLEAAGILWRLLARGIA
                     RGGPRRAWHFLRSLPLVRPHLVPLAVNDWIAGLAMRDYAERHFGLPASRAVMSPEGAL
                     ARLRRSLLGWRRRGAVRLTLHRPGHAEPQIAVRVTGALDRALARRLARQLGAVLRRSR
                     TRVVLALESLGPPQELERLTRTLGRHGDRLLIVVGEGLRELLRCEPALPAPQN"
     gene            4413..6503
                     /locus_tag="AC16O9_14980"
     CDS             4413..6503
                     /locus_tag="AC16O9_14980"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HIR6517957.1"
                     /translation="MLPSAVIKRDGTLAPFDATKIAAALARAGAATGEFDADQAARLA
                     AQAVKVIAHRFAAATPTIENIQDVAEQVLIAADHLATARAYIVYREQHKRLRADRRTV
                     VDVAASMNEYLERQDWRVNANANQGYSLGGLILNVAGKVTANYWLSHVYPPEAGQAHR
                     DGDLHIHDLDMLAGYCAGWSLRTLLAEGLNGVPGKVEAAPAKHLSSAVGQIVNFLGTL
                     QNEWAGAQAFSSFDTYMAPFVRKDRLGYAEVKQCIQELVYNLNVPSRWGTQTPFTNLT
                     FDWTCPEDLRGQAPVIGGEEMPFTYGELAPEMAMINRAYIEVMTAGDAKGRVFTFPIP
                     TYNITKDFDWDGPNTQALFAMTAKYGLPYFQNFVNSDLEPHMVRSMCCRLQLDLRELL
                     KRGNGLFGSAEQTGSVGVVTINCARLGHRFQGDERALLERLDQLLEIGRTSLEIKRKV
                     IQRHMDAGLFPYTKRYLGTLRNHFSTLGVNGLNEMVRNFTAGAHDITSAWGHAFACRV
                     LDHVRARMVEFQEETGHLYNLEATPAEGTTYRFAKEDRKRFPGILQAGSPDMPYYTNS
                     SQLPVGFTDDPFEALERQEELQSKYTGGTVLHLYMTERISSAEACKALLRRSLERFRL
                     PYVTVTPTFSICPGHGYIAGEHETCPQCGSACEVWTRVMGYHRPVASFNKGKQGEFRE
                     RRFFEEDKCCASAG"
     gene            6482..6973
                     /locus_tag="AC16O9_14985"
     CDS             6482..6973
                     /locus_tag="AC16O9_14985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR6517958.1"
                     /translation="MLRVGGLTRLSACDYPGKLAAVVYCQGCAWRCGYCHNPELQPAR
                     GAYEIPWPEVAAFLEKRRGLLDAVVFSGGEPTLQRGLADAMREAKAMGYLVGLHTAGA
                     VPRRLAEVLPLVDWVAMDVKAPFDEHEAVTCVRGSGARARLSAELLRASGVARELHTV
                     SAA"
     gene            complement(6970..7908)
                     /locus_tag="AC16O9_14990"
     CDS             complement(6970..7908)
                     /locus_tag="AC16O9_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR6517959.1"
                     /translation="MPRAEPTLEAFSTPLARYFAATRPAFLSVTLVGCLLGLASAYGG
                     GMALDPATAFVTLFFALVAHAGINVLNDYYDALNGSDAANAERRYPFTGGSRFIQNGL
                     ISVSATGIFGYALLMAVVPAGLWLAHASGPGLLGIGLAGLMVGWAYSAPPFKLMSRGL
                     GEAAVACGWLIVVLGADYVQRGAFSSAPLVAGLPYALHVANLLFTNQFPDVKADAAAG
                     KRNLVVRFGPRRARWLYPAIAALAYLWLIGAVLTGALPVLALVALAAAFPALSAARVL
                     ARNALRPAALDPAIRATLGAALMHGALLAAALLAAR"
     gene            complement(7909..9333)
                     /gene="cysG"
                     /locus_tag="AC16O9_14995"
     CDS             complement(7909..9333)
                     /gene="cysG"
                     /locus_tag="AC16O9_14995"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HIR6517960.1"
                     /translation="MSIAPVAERAYFAAFLDLDGKPGTVVGGGTVAALKAEALLRSGV
                     RVTVVAPELCPRLTELTLLGAVRHEPKRFAPGDLVGAEIAIAASDDTEVNEAVSVAAR
                     ALRIPVNVADDAKRSSFIMPSVVDRPPLQIAISSAGASPVLARKLRTIIEAAVPFAFG
                     RLAALAGRFRAASRRRLPDPEARRRFWEEVMDGPVAELMLAGREDEAARALEQRLEDG
                     TPPAGGFVSLVGAGPGDPELLTLRALRVMQRANVVLYDHLVAPALVDLARRDAERIYV
                     GKEHDNHALPQEDINALMVRLAREGKRVVRLKGGDPFIFGRGGEEIESLAAQGIGFEV
                     VPGVTAAAGAAAYAGIPLTHRDYAQSCVFVTGHLKKGAIEFDWRAIARPRQTVAVYMG
                     VGGVQAICRGLVEHGLAPDLPAALVENATLAKQRVIEGTLATLPAAAAAAAVRPPALL
                     IVGEVVRLRGRLAWFERAPQVSVA"
     gene            9415..9732
                     /locus_tag="AC16O9_15000"
     CDS             9415..9732
                     /locus_tag="AC16O9_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6517961.1"
                     /translation="MHRIALALALAAAATTALPADPAPARRAELLHLVRQDCGSCHGL
                     TMKGGLGPSLEPAALAGKDANMLRFVILQGRHGTAMPPWRAHVSEAEAGWIVEQLKKG
                     LPK"
     gene            9729..10871
                     /locus_tag="AC16O9_15005"
     CDS             9729..10871
                     /locus_tag="AC16O9_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR6517962.1"
                     /translation="MKRLVSAALALALAACAAPRGTGDLGLVVERAAGRVMLIETSGR
                     TALGRVTGLGDLSHAAAVFSRDGRYAYVFGRDGGLTKVDLLRARIDKRIIQAGNSIGG
                     AISQDGSLVAVANYSPGGIRLFDARTLEPLAEVPTGAKVVGIADAPGNRFVFSLFEAG
                     EIWIVDASQPRAPAVTRLSNIGRQPYDGFVTPNGRHYIAGLFGEDGLALVDLWNPGMG
                     ARRILDGYGRGEAPLPVYKMPHLRGWAVAGRYIFLPAIGRHEVLVLDSVTWTEAGRIA
                     VHGQPVFAVAQPGGRRVWVNFAFPTNDVVQVIDVPQMQVVQELRPGRAVLHMEFTPRG
                     EQAWVSSRDDGAVVVYDGASFAELARLPAESPSGIFLTSRGAMTGF"
     gene            10873..11820
                     /gene="ahbB"
                     /locus_tag="AC16O9_15010"
     CDS             10873..11820
                     /gene="ahbB"
                     /locus_tag="AC16O9_15010"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit beta"
                     /protein_id="HIR6517963.1"
                     /translation="MFDAESFRLVNAWQRGFPLVPRPFAAIARASGFDEGETIARFAQ
                     LKSRGLIDRIGPVFRPNTVGASTLAAMSVPAERIEGVAQQVSAHPGVNHNYEREHAIN
                     LWFVATAPSAAALQNVLGAIEEETGLAVLRLPLTEEFHIDLGFDLEDHSVPRTAGAAP
                     VAPLPEAERALAAHLTAGLPLVPRPYAAFGVPETDLIAALERWLATGIVRRIGAVVRH
                     RPLGYEANAMVVWDAPDAEASDAGRRLAADPAVTLAYRRARALPAWPFNLYCMVHGRE
                     RAAVTQSIERLSAQHGVSGYPRAVLFSQRCFSQRAAHYA"
     gene            11813..12256
                     /locus_tag="AC16O9_15015"
     CDS             11813..12256
                     /locus_tag="AC16O9_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6517964.1"
                     /translation="MRELERAVVNRLQGGFPLAPRPFAAAAAELGTDEATLIETLRAM
                     LAAGTLTRFGPLFDAERLGGAFTLCAMSVPASDLERVVAQVNAHPEVAHNYAREHRFN
                     LWFVLATGARARIAEVIATIEAETGYPVLDLPREREYFIDLRLTA"
     gene            12253..12723
                     /gene="ahbB"
                     /locus_tag="AC16O9_15020"
     CDS             12253..12723
                     /gene="ahbB"
                     /locus_tag="AC16O9_15020"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit beta"
                     /protein_id="HIR6517965.1"
                     /translation="MKATDEAIVRATQGGLPLVAEPYAALARELGVAQDELCARLAAM
                     LEDGRIRRIGAVPNHYALGYKANAMTVWDVADDAVDELGAAVGALEFVTHCYRRPRRL
                     PDWRYNLFAMVHGRSRAETEAHRAAIRAQLGGALAADDVLYSTRILKKTGLRLA"
     gene            12725..13873
                     /gene="nirJ"
                     /locus_tag="AC16O9_15025"
     CDS             12725..13873
                     /gene="nirJ"
                     /locus_tag="AC16O9_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HIR6517966.1"
                     /translation="MFRVSRFMEEILQPAPPARRGSGTPLGPVVIWNLIRRCNLTCMH
                     CYSISADIDFPGELSTAEVLGVMDDLKAFNVRALILSGGEPLLRRDLFEIAARGKALG
                     FYLGLSTNGTLIDAPLARRIADAGFDYVGISLDGLQATHDRFRRKAGAFDASLAALRR
                     CRDLGVKVGVRFTLTQDNFADLPGLLELVEAEGIPRFYLSHLNYAGRGNVNRKRDAHF
                     RVTREALELLYARAWDAAQRGLHKDFVTGNNDADGVYALYWARRNVPEFDEPRLRASL
                     AAWGGNASGVNVANIDNLGNVHPDTMWWDCTLGNVRARPFSAIWQDTRHPVMAGLKER
                     PRRVKGRCAGCAYFDVCGGNTRVRAMQVTGDPWAEDPGCYLEDAEIAA"
     gene            13870..15378
                     /locus_tag="AC16O9_15030"
     CDS             13870..15378
                     /locus_tag="AC16O9_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR6517967.1"
                     /translation="MRALLAAALLAPALAWPADLYETHCASCHGAARLGAMGPALLPE
                     SLGRLKKAEAARVIRDSRPGVQMPAFSKTLSEAEIAQLVELIYRPVVPAPRWGEAEIR
                     ASRVVHHAPGALPAKPQFDADPLNLFVVVEAGDHHVTILDGDRLEPLARFASRFALHG
                     GPKFSPDGRYVFFASRDGWITKYDLWNLKTVAEVRAGLNTRNAAVSADGKYVMAANFL
                     PHSLVVLDAALELVKVLGTDSRVSAVYDAAPRRSFVAALKDVPELWEISYDPKAEPIA
                     GGLVHDYRFKEGAFVPGFLNARRTRLDQVLDDFFFTPDFRQVVGASRGGKRGQVVHLD
                     VRRTIAEVDLPGLPHLASGITWERRGRRVMATPNLREAVVSVIDVEGWKTVAEIPTLG
                     PGYFLRSHEATPYAWVDSMIGPQGRDTLQVIDKDTLEVVARLTPAPGKTLAHVEFDRH
                     GRYALASLWEAEGALIVFDARTLAEVKRIPMRKPVGKYNVHNKITRSEGTSW"
     gene            15429..16067
                     /locus_tag="AC16O9_15035"
     CDS             15429..16067
                     /locus_tag="AC16O9_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6517968.1"
                     /translation="MFGEMSAAELDRIAASTLPLHVDKGETVCHTGDPCTGFHVVVYG
                     QVKLGFTSPQGVEKVVEIVRPGQSFGEALMFLDAPYIVFAQALADSMLLHVAKHAVLD
                     ELGRDPGFARRMLSGLSQRLHGLVRDVEAYSLRSSHERVIGYLLAEAPVDANGAPVEV
                     QLSAGKSVIASRLNMTPEHFSRILHELGNDGVIEIRGRTVRIPNLARLRGSA"
     gene            16144..17133
                     /gene="ubiU"
                     /locus_tag="AC16O9_15040"
     CDS             16144..17133
                     /gene="ubiU"
                     /locus_tag="AC16O9_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiU"
                     /protein_id="HIR6517969.1"
                     /translation="MELVCPAGALPALKAAVDNGADWVYLGLRDDTNARNFPGLNFDE
                     SALVAGLAYAHARGRKVLMAVNTYPQAGSWARWTAAVDRAARLGVDAIIVADAGLMRY
                     ASRTHPRLELHLSVQASATSYEAINFFHEHFGVKRAVLPRVLSLAQVAQVARHTPVEI
                     EVFGFGSLCVMVEGRCTLSAYACGEAPNTTGACSPAHAVRWRETAAGLESRLNGILID
                     RYAPGERAAYPTVCKGRYAATGETYYAFEEPASLNTLDLVPQFGEAGVRAVKIEGRQR
                     SPAYVAQVTRVWREAIDAWKAGGFAPRPAWAAALQRHAEGSHTTLGAFSRPWK"
     gene            17130..18014
                     /gene="ubiV"
                     /locus_tag="AC16O9_15045"
     CDS             17130..18014
                     /gene="ubiV"
                     /locus_tag="AC16O9_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiV"
                     /protein_id="HIR6517970.1"
                     /translation="MKLALGPLLYYWPRDAVLAFYEEIAAAPVDTVYLGEVVCSRRHE
                     LAFEDWLAIAARLAAAGKEVVLSAQALTESEGDLKLVRRAVANGRFRVEANDWGAVRL
                     LAAVTGWIAGPHLNVYNPQTLDLVTALGATRWVAPTEATRDMVAGLLAERPAGLEAEV
                     FAYGRMPLAFSARCFTARRLNRQKEDCGYACLDFPDGIALKTREGEPFLAVNGIQTLS
                     AGVYCLVDELAALRETGVEVLRVSPQSVGTHDVLHILRAACDDALSGFDARKALAAVA
                     PGKLWNGFWHGQPGMPAR"
     gene            18011..18304
                     /gene="ubiT"
                     /locus_tag="AC16O9_15050"
     CDS             18011..18304
                     /gene="ubiT"
                     /locus_tag="AC16O9_15050"
                     /inference="COORDINATES: protein motif:HMM:NF047962.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis accessory
                     factor UbiT"
                     /protein_id="HIR6517971.1"
                     /translation="MIPLSDRLHCMLAPLRGKVLRLEIAGLGGGPQFTLDFIGLRPSF
                     GKPDVTVRASLGDYLALALRREDPDTLFFTRRLVIEGDTELGLQIKNALDALG"
     gene            complement(18357..18665)
                     /locus_tag="AC16O9_15055"
     CDS             complement(18357..18665)
                     /locus_tag="AC16O9_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6517972.1"
                     /translation="MKIVIVALTAAAAVLSTPARADEALAKKHNCLACHQPDKKLVGP
                     SYKDIAKKYKGQASAAGMLADKVKKGGSGAFGPVPMPPNPAVPDADIKTLVDWILKMG
                     "
     gene            complement(18682..20409)
                     /locus_tag="AC16O9_15060"
     CDS             complement(18682..20409)
                     /locus_tag="AC16O9_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR6517973.1"
                     /translation="MTNPNIRLTRAAAWLLLPLAAATAYGQAAKHPTTESEIRYQAGG
                     SPLAAEDMHQDVNPKAPPMSKAEFDRARVLYFERCAGCHGVLRKGATGKPLTPDITLP
                     KGTEYLKVFIKYGSPAGMPNWGTSGDMSDDEVDLMARYVQQTPPTPPEFGMADMKKTW
                     KVIVPPAQRPKKKMNKFNIANIFSTTLRDTGEVALIDGDTKKIINIVKTGYAVHISRV
                     SASGRYVLVIGRDAKINMIDLWMEKPDNVAEIRIGLEARSVDTSKAKGYEDKLAIAGA
                     YWPPQYVIMKGDTLEPLKIVSTRGMTVDTQEYHPEPRVAAIVAEHRRPHFVVNVKETG
                     KIVMVDYTDLKAITSTEVEAARFLHDGGWDASKRYFLVAANQSNKIAVLDTKTDKLAA
                     LIDVDKIPHPGRGANFVHPKYGPVWATGHLGSEKIALIGTDPAKNKQHAWKVVQMLDG
                     QGGGNLFIKTHPKSKNLWVDTPLNPDPKISQSVAVFDIGNLGKPAVVLPIGEWANLGE
                     GAKRIVQPEYNKAGTEVWFSVWSAKNQESALVVVDDKTRKLKAVIKDPRLITPTGHFN
                     VYNTQHDIY"
     gene            complement(20487..20972)
                     /locus_tag="AC16O9_15065"
     CDS             complement(20487..20972)
                     /locus_tag="AC16O9_15065"
                     /inference="COORDINATES: protein motif:HMM:NF021520.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HIR6517974.1"
                     /translation="MVQSADGLIEAALAAARAGDWATHRARFAQLRQACAALLERDED
                     LCQRFDTLGAAAPQHDPEGCRAAFEELAALLRERHADALRAGRAPADSPTLDLRGLQP
                     PEPIVRIFAALERAPGESLRAILPHEPLPLYPLLRERGFSYSGAQRADGAFELLIEPS
                     T"
     gene            complement(21017..21721)
                     /locus_tag="AC16O9_15070"
     CDS             complement(21017..21721)
                     /locus_tag="AC16O9_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517975.1"
                     /translation="MERAPARVTSAWSAAGLGLVLVVLFASLAIRHASDELGAVLGAV
                     RGVHRAAASLEVVAALALTWLAWRRALGVAVLTVFLSVLGIAVGQNPPPAAAAANLLG
                     GLALAAAFAWLLGRAQGHAARAARRLGHAVAALFVAQCALGAWLAIFAEDIWGWVLAG
                     HVALGLGLAAGAAWLALRLERAAQRFALLGLALAVVAAGAAAALFGQPLAASLAHGAA
                     GALLAAAAAYAHARLT"
     gene            complement(21697..22674)
                     /locus_tag="AC16O9_15075"
     CDS             complement(21697..22674)
                     /locus_tag="AC16O9_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR6517976.1"
                     /translation="MTDSARRQVAAWLLLCAAMVFAIVLVGGVTRLTRSGLSIVEWQP
                     LIGVLPPLTEAHWAELFAKYRETPEFRLVNFAMDLEGFKRIFWWEYVHRLLARAIGLV
                     FLLPLIWFQLKKKLEPGLAWKLWGIFALGALQGLMGWLMVASGLIDDPKVNPVRLTLH
                     LGLALAIFSAELWIALGLLWPRAGRAPRLAAALPFAVFVMALSGGMVAGLRAGAAYNT
                     FPLMNGHWVPPEILMLEPWWRNFLYNMATVQFVHRGLFWLLAVLVPIAWWQRRTHWAA
                     HALLGAFVLQATLGIATLLAGVPVALGAAHQGGAVLVLAAAIWNAHRHA"
     gene            22782..22952
                     /locus_tag="AC16O9_15080"
     CDS             22782..22952
                     /locus_tag="AC16O9_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517977.1"
                     /translation="MNAERIEHDEVPDEPVPAMQQLLDNPFLLLFLGIAMPTVLYIVW
                     GVMEIVSLPIAK"
     gene            22963..23508
                     /locus_tag="AC16O9_15085"
     CDS             22963..23508
                     /locus_tag="AC16O9_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HIR6517978.1"
                     /translation="MSAILPPQDRVWWKQPLDRVELLWIVVALIWCLIMFFMMPYWHV
                     YGQQNLSNEAYRSKPEDFVKKTEAMIAQYKVREDGGDLKVPVVRPPAGSDVYLVAQLW
                     RWYPILELEAGKSYRIHLMSLDWLHGFSLQPENINIQVHPGYEHVLTVTPTNPGTYSI
                     ICNEYCGINHHTMVSRLYVVK"
     gene            23521..25245
                     /locus_tag="AC16O9_15090"
     CDS             23521..25245
                     /locus_tag="AC16O9_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR6517979.1"
                     /translation="MSVNRMFRTCPSSGLVFHEPAEKLMRWNAVAAVVSLLIGGLLAI
                     GVVLTRWQAVHLLPPDRFYQYLTAHGLNMLVFWIIFFEIAVLYFASSTLLKSRIATPA
                     MAWLGFWLMVLGALVNNYSVFRGDSSVMFTSYAPMGAHPTFYLGLILFAVGALIACFV
                     FFGTLVVAKAERTYHGSVPLVTFGAITAGIIAVFTIAAGAIILIPTFLWSVGLVEHID
                     SLMYRTVWWAFGHSSQQINVSAHVAVWYAVAAIVFGAKPLSEKVSRTAFLLYILFLQL
                     ASAHHLLADPGLTSHWKIFNTSYAMYLAVLASMVHGLTVPGSIEVAQREKGFNHGLFE
                     WLRKAPWGNPAFSSMFMSLIGFGFLGGISGVMMGTEQLNLIIHNTLYVPGHFHATVVV
                     GTTLAFMGLTYFLIPVLFRRELAFPGMAKWQPYVFGLSMMGVSLFLMGAGTLGVPRRH
                     WDIGFAGNAFAHEFPGAAWLMMALAGITGLIAIVGGGMYLLITVYSVFLGKKIETPGF
                     QHVRAKDGIAPAPKPVAAAIAGHHPNVGAGGLVAPGTFALAMVFLASFVLYYFINWKY
                     LSTVWPLK"
     gene            25242..26096
                     /gene="cyoE"
                     /locus_tag="AC16O9_15095"
     CDS             25242..26096
                     /gene="cyoE"
                     /locus_tag="AC16O9_15095"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR6517980.1"
                     /translation="MRQVLNLFKLRIGVAIAVAAAAGMAVESGAGPGAAAGAMLILAV
                     LVASAAAGAFNQYVERDLDARMGRTRNRPFVTGALKHGPAWLWLIGAMLALSVLAAAV
                     AANPLAALYVFLGAFTYGVVYTVWLKRRTWWNIVVGGLAGSFAVLAGAAAVSPAPGPE
                     ALALAVVLFLWTPPHFWSLAIVCREDYAKARVPMLPVVVGDARAARAIFASAALLVGA
                     SLVPAAFGLGWIYLSFAAAGGILFLLRCRALVRRPGRGAARAAFLASLLQLGAVLAGA
                     MLDAALLA"
     gene            26077..>26547
                     /locus_tag="AC16O9_15100"
     CDS             26077..>26547
                     /locus_tag="AC16O9_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6517981.1"
                     /translation="MLRFSLSALLLAAALGAHAFDEKLALRESQAAIGRPIGDYTLRD
                     TEGRTVRLAELRGKPLVVNFVYTGCFQICPASTQFLAKAIAEAERTLGPGTFRVATIG
                     FNLPFDTPGAMKEFARKHAVESPHWLFLSPEAETLPALVSEFGFRYESTTAGFDH"
BASE COUNT         3889 a         9538 c         9170 g         3950 t
ORIGIN      
        1 acctcggcga gccgggtcgc cgccgggtcg cgtccctcgg ccatgacgcg attgagcgcg
       61 tcgcgctccg tgaagatgcc gaccagctgc tcgccgcgca ccaccagcac ggcgccgacg
      121 tggctcacgc gcatctgccg cgccgcgtcc aggacgctcg tgtcgggaga cgcgaccacg
      181 agcctgcgcg actcgatcag ttcgcccacg gcggtttcga gcatgcgggc cagcgtacac
      241 gttgcgtatg ccccgccttt gatgcggatc aagcggagcg gttgatgcag gtcaacggca
      301 cgggccgacc gcggtctagc atggcccggt aatgcttacg accggcgccc tcctgctcgc
      361 cgcggtcgcc ctcgcgctgg gaatcgtgct gcacctgcga cggcgccgcg cgcgcaccga
      421 cgaccgcatg cgcctctacg acataatgcg ccgccgcggc gtatcgccgc cgccggccga
      481 ggacgccgtg ggcgcgcagg acgtcgccgc cgccgagcgc cgctgtgccg cgtgcgcaaa
      541 caaggagatg tgcgacgagc tgctgcgcat cggccagacc cggggctacg accgtttctg
      601 ccccaatgcc ctctatatcg agtggctgcg cagcaacagc ctggacttcg aactgccgcc
      661 gcccccctca gcaggccgtc cggactgagc gcgcgcgccg cacggcgagc gtcgccgcgg
      721 tggcgatgaa cagcgcgacc gccgccgcgt tgccccaggc tcccgccagc ggcgacacga
      781 aaacgcgcag cgcgagcgac aggtgcagca gggcaagcgg gacgtagagc gccgcgtgat
      841 acggcagcgg cacccgcagc accgccggca ggatcaccgg cgcgtggccg aacaccatcg
      901 agaacacgaa gccgacgaag atcgcgtgca gcgccgcgtc gtacgcggtc agcgtcgcca
      961 gcagcgctcc gccgaacgca agccaggcgt agcccgagag caggcacacg gcgatgtagc
     1021 gcggcaggcc gcgctggcgc acggtgacgc gcgcaaggtc gaaggcgaac agccacgccg
     1081 cgagcagcac cagcaccagc cccagcaccc ggggaaacac cggcgcggcg aggagcgcgg
     1141 cggccagcag gacgaaggaa ccgcgcgcga aggcgccgcg gcgcaggtag cgcgacagct
     1201 cgaggcgctc gccaccaatg gtgagcgcga ggaacgcgat ccaccacggc accgcggccg
     1261 cgccggcgta cagcgccagg ttccccgcca gccacgccac ggcgccggcg gcgagcgtcg
     1321 ccatctcggg agacggctgg cgcgcgagca ccgccaggct cgcggcggcg agcagcgccg
     1381 cggcgagcgc gagcagcgcg aaacccggcg cggcgaagcc caggatcagg cacagcccgc
     1441 cgagcccgct cgccagcggc gcggcatagg gccaaagccg gccgagcgct acagcgcgct
     1501 cgagcccgat caccgtgcca aggaaccctg agaccatcag cgcgccgtgc aacgcgatcg
     1561 cgcccgccgg cgcgggcagg gcggcgccca ggcgcgccag accgcccgcg atgcccaagg
     1621 cgagcgacgc gaagccaagc accagcagcg gtacgcgcca gcgcggcgcc aagcgctagc
     1681 ccgtccagcc gaagcggctc tgcgcctcgg cgctcatgcg ctccggggtc cagggcggat
     1741 cccagaccag ttccacggcc acgtcggttc ccgccggcgc gagcgcgcgg atcgccgcca
     1801 tcgcctcgtc cacgaggtat ggcgccgcgg ggcacgcggg cgaggtcatt gtcatctgca
     1861 cgcgcacgcg cccaggcgcg gcctgcacgc cgtagacgag gccgaggtcg acaacgctca
     1921 tcccggcttc cgggtcctcg acgccgcgaa gcgcctgcat gacttctttc tcgtccatgc
     1981 ggccagtaaa gcgcatcgcg tccgcaactc cttgatttgc atcaatccgc cggggtgccg
     2041 cggcgctatc gtagacacat gcacttcatg ctggtgatcc tgatcttcct cgtcggcgcg
     2101 gtgctctggg gctacttcta cgccaacccg cagggcgtat cgcgcggcaa gctcctcgcc
     2161 tgcaatatcg cggtgctcgc gctcggcgcc gcgatcgctg cggccgcggg cgtgctgctg
     2221 ctcgaggacg ggctgcgcgt caagcccggc gagccgggcc tggcctggtt cctcgccatc
     2281 atgggcgccg gcacggcgtt catgatcgtg gtcgcgagcg gcgggctggt gcgcaacctg
     2341 ctcctgttcc cgctttccaa gcgctcgcgt tgaccggcgt caactcctgg ccgcggcgcg
     2401 cgcgctgcgt cgctagcatc caccgatgcg attgctgctc gtcaatccgg cgctgccgga
     2461 gagtttctgg agcttccgct gggcgctgga gcgcgtgctc ggcggcaagc gcgcgctcaa
     2521 cccgcccctc ggcctcgcca cgctcgccgc cttgtgcccg ccgcactggc aggtgacgat
     2581 cgtcgacgag aacgtcgagc cgctgccgct cgaaccgcac gccgatctga tcggcatcgg
     2641 cggcatgggt gtgcaggcgc cgcggcaaaa gcagctgctg cgctactacc gcgaggccgg
     2701 ctaccgcgtg gtcgcgggcg gcagctacgc ctcgctgtgc cccgaggagt accgcgagca
     2761 cgccgatcac gtcatcgccg gcgaggccga gtacatctgg ccgaagttct gcaccgacct
     2821 cgagcgcggc gtggcgcagg cgctgtatcg cgagaccggc gaagttcggc tcgaggattc
     2881 gcccgtgccg cgcttcgacc tgctgaagct cgacgcctat tcgaccgcga gcctgcagct
     2941 ctcgcgcggc tgcccgttcc gctgcgagtt ctgcgacatc atcgtcatgt tcgggcgcaa
     3001 gccccgctac aagactcccg agcgcatcga gcgcgagctc gacagcctgc ggcgcctggg
     3061 cgcgcgcaag gtgttcttcg tcgacgacaa cttcatcggc aacaaggcgc gggcgaagga
     3121 gacgctgcgc tgcgtcgccg cctaccaggc gcgtcacggc tggcgcatgc gctttggcac
     3181 ggaggcctcg ctcaacctcg ccgacgaccc cgagctgctc gcgctatttc gcgaagcggg
     3241 gttcgagtgg gctttcctcg gcctggaaac gcccgacccg cagaccttgc gtgaagcggc
     3301 gaagtcgcag aacaccgccg gcgacatgct cggcgcggtg cgccgcatct acgccgcggg
     3361 cgtcgacatc ctcggcggct tcatcgtcgg cttcgaccgc gatacgcctg cagcattcga
     3421 aaagcagcgc cgcttcattc tcgacagcgg catcatggtg gccatggtcg gactgctcac
     3481 cgcgctgccc aagaccccgc tctacgagcg cctgaagcgc gaggggcgcc tgttggagcg
     3541 cgccgatcac ggcgacaaca cgcgcgcgcg caccaacatc gtgcccaagt cgatgagcgt
     3601 cgcgcagctc accgcaggct accagcggct ctacgcccgg ctgctcaccg acggcgccat
     3661 tgccgagcgc atccgcaaca agctggcgca cttcggcgcc tcggcgcgcc tggtgcgcga
     3721 gacgccgctc gaggccgcgg gcatcctgtg gcggctgctc gcgcgcggca tcgcgcgcgg
     3781 cggcccgcgc cgcgcctggc atttcctgcg cagcctgccg ctcgtgcggc cgcacctggt
     3841 gccgctcgcc gtgaacgact ggatcgccgg cctcgccatg cgcgactacg ccgagcggca
     3901 tttcggcctg ccggcgtcgc gcgccgtgat gagcccggaa ggcgcgctgg cgcggctgcg
     3961 ccgctcgctg ctcggctggc ggcgccgcgg ggccgtgcgc ctcacgctgc accgccccgg
     4021 ccacgccgag ccgcagatcg ccgtgcgcgt caccggcgcg ctcgaccggg cgctggcgcg
     4081 gcgcctcgcg cgccagctcg gcgcagttct caggcgctcg cgcacgcgcg tcgtgctcgc
     4141 gttggagtcg ctcggcccgc cgcaagagct ggagcggctc acgcgcacgc tcggccggca
     4201 cggcgatcgc ctgctgatcg tcgtcggcga gggcctgcgc gaattgctgc gctgcgagcc
     4261 cgcgcttccc gcaccccaga attgatccgc gtcaaccgcg ggcattgact gcgccctgcc
     4321 ggacgccggt agagtgtggc ctccaataac aagatgttgt ggaggaaatc gtggaaacca
     4381 ctataagtag cgctccccgg gctgcggcca ccgtgctgcc gagcgcggtg atcaagcgcg
     4441 acggcacgct cgcgcctttt gacgccacca agatcgccgc cgccctggcg cgcgccggcg
     4501 ccgcgaccgg cgagttcgac gcggaccagg cggcccgcct cgccgcgcag gcggtcaagg
     4561 tgatcgcgca ccgctttgcg gccgcgacgc cgaccatcga gaacatccag gacgtcgccg
     4621 agcaggtgct gatcgccgcc gaccaccttg ccacggcgcg cgcctacatc gtctaccgcg
     4681 agcagcacaa gcggctgcgc gccgaccggc gcacggtggt ggacgtggcc gcgtcgatga
     4741 acgagtacct cgagcgccag gactggcgcg tgaacgccaa cgccaaccag ggctattcgc
     4801 tcggcggcct gatcctcaac gtggcgggca aggtgacggc gaactactgg ctgtcgcacg
     4861 tctatccgcc ggaagccggc caggcgcacc gcgacggcga cctgcacatc cacgacctcg
     4921 acatgctcgc cggctactgc gccgggtggt cgctgcgcac gctgctggcc gaggggctga
     4981 acggcgtgcc gggcaaggtc gaggccgcgc cggcgaagca tctgtccagc gccgtgggcc
     5041 agatcgtcaa cttcctcggc acgctgcaga acgaatgggc gggcgcccag gcgttcagtt
     5101 cgttcgacac ctacatggcg cccttcgtac gcaaggaccg cctgggctac gccgaggtaa
     5161 agcagtgcat ccaggagctg gtctacaacc tgaacgtgcc ctcgcgctgg ggcacgcaga
     5221 cgccgttcac caacctcacc ttcgactgga cctgccccga ggacctcagg ggccaggcgc
     5281 cggtgatcgg cggcgaggag atgccgttca cctacggcga gctcgcgccc gagatggcga
     5341 tgatcaaccg cgcctacatc gaggtgatga ccgcgggcga cgccaagggg cgcgtgttca
     5401 ccttcccgat tccgacctac aacatcacca aggatttcga ctgggacgga ccgaacacgc
     5461 aggcgctctt cgccatgacg gcgaaatacg ggctgcccta cttccagaac ttcgtgaact
     5521 ccgacctcga gccgcacatg gtgcgctcga tgtgctgccg gctgcagctg gacctgcgcg
     5581 agctgctcaa gcgcggcaac ggcctgttcg gctctgcgga gcagactggg tcggtgggcg
     5641 tggtcacgat caactgcgcc cgcctgggtc atcgcttcca gggcgacgag cgtgcgctgc
     5701 tcgagcggct ggaccagctc ctcgagatcg ggcgcacgag cctcgagatc aagcgcaagg
     5761 tgatccagcg ccacatggac gcgggcctgt tcccatacac gaagcgctac ctcggcacgc
     5821 tgcgcaacca tttctcgacg ctcggcgtga acggcctgaa cgagatggtg cgcaacttca
     5881 ccgccggcgc gcacgacatc acgagcgcgt gggggcatgc attcgcctgc cgcgtgctcg
     5941 atcacgtgcg cgcgcgcatg gtcgagttcc aggaggagac cgggcacctg tacaacctcg
     6001 aggcgacgcc ggccgagggc acgacgtacc gcttcgccaa ggaggaccgc aagcgcttcc
     6061 ccggcatcct gcaggcgggc agccccgaca tgccctacta caccaattcg tcgcagctgc
     6121 cggtaggctt caccgacgat ccgttcgaag cgctcgagcg ccaggaggag ctgcagagca
     6181 agtacaccgg cggcaccgtg ctgcacctgt acatgaccga gcgcatcagc tcggccgagg
     6241 cgtgcaaggc gctgctgcgc cgctcgctcg agcgcttccg cctgccctac gtcacggtga
     6301 ctccgacctt ctcgatctgc ccggggcacg gctacatcgc cggcgagcac gagacctgcc
     6361 cgcagtgcgg ctcggcctgc gaagtctgga cccgcgtcat gggctaccac cggccggtcg
     6421 cctcgttcaa caagggcaag cagggcgagt tccgcgagcg ccgcttcttc gaggaggaca
     6481 agtgctgcgc gtcggcgggc tgacccgcct ttcggcctgc gactaccccg gcaagctcgc
     6541 cgcggtggtc tactgccagg gctgcgcctg gcgctgcggc tactgccaca accccgagtt
     6601 gcagcccgcg cgcggcgcgt acgagattcc gtggcccgag gtggcggcct tcctcgagaa
     6661 gcgccgcggc ctgctggacg ccgtcgtctt cagcggcggc gagcccaccc tgcagcgcgg
     6721 tctcgctgac gcgatgcgcg aggcgaaggc gatgggctac ctcgtcggac tgcacaccgc
     6781 gggcgccgtg ccgcggcgcc tcgccgaagt gctgccgctc gtggattggg tggcaatgga
     6841 cgtgaaggcg ccattcgacg agcacgaggc cgtaacgtgc gtgcgcggca gcggcgcacg
     6901 cgcgcgatta agcgcggagc tcctccgcgc gagcggtgtc gcccgcgagc tccataccgt
     6961 gtcggccgcc tagcgcgccg cgagcagcgc cgccgcgagc agcgcgccgt gcatcagcgc
     7021 ggcgccgagg gtggcgcgga tcgcggggtc gagcgccgcc ggccgcagcg cgttgcgcgc
     7081 cagcacgcgc gcggcgctca aggcgggaaa ggccgcggcg agcgcaacga gcgcaagcac
     7141 cggcagcgcg ccggtgagca ccgcgccgat cagccacaga tacgccagcg ccgcgatcgc
     7201 ggggtagagc cagcgtgcgc gccggggccc gaaccgcacc acgaggtttc tcttgccggc
     7261 ggcggcatcg gcctttacgt ccgggaactg attggtgaac agcaggttcg cgacgtgcag
     7321 cgcatacggc aggccggcga cgagcggcgc cgacgagaac gcgccgcgct gcacgtagtc
     7381 cgcgcccagc accacgatca gccagccgca ggccactgcc gcctcgccca agccccggct
     7441 catcagcttg aacggcggcg cggaatacgc ccagcccacc atgaggcccg cgaggccgat
     7501 gcccagcaac ccggggccgg acgcatgcgc gagccagagc ccggccggca cgaccgccat
     7561 gagcagcgcg tagccgaaga tgccggtggc gctcacgctg atcagtccgt tctggatgaa
     7621 gcggctaccg ccggtgaaag ggtagcgccg ctcggcgttg gcagcgtcgc tgccgttcag
     7681 tgcatcgtag taatcgttca gcacgttgat gccggcgtgc gccaccagcg caaagaacag
     7741 ggtgacaaac gcggttgccg ggtcgagcgc catgccgccg ccgtacgcgc tcgccagccc
     7801 gaggaggcag ccgacgaggg taacgctgag gaacgccggg cgcgttgcgg cgaaatagcg
     7861 tgcgagcgga gtggagaatg cttcgagcgt cggttcggcg cgcggcattc aggcgaccga
     7921 gacctgcggc gcgcgctcga accacgcgag ccgcccacgc aggcgcacca cctcgccgac
     7981 gatgagcagc gccggcggcc tcacggcggc ggcggccgcc gccgcgggca gcgtggcgag
     8041 cgtgccttcg atcacgcgct gcttcgcgag cgtcgcgttt tccaccagtg ccgcgggcag
     8101 atcgggcgcg aggccgtgct cgacgaggcc gcggcagatc gcctgcaccc cgcccacgcc
     8161 catgtacacg gcgacggtct gccgcggtcg cgcgatcgcg cgccagtcga actcgatcgc
     8221 gcccttcttc aggtgcccgg tgacgaacac gcaggactgc gcgtagtcgc ggtgcgtcag
     8281 gggtatgccg gcgtaggcgg ccgccccggc cgccgcggtc acgcccggca cgacttcgaa
     8341 gccgatgccc tgcgcggcga gcgactcgat ctcttcgccg ccgcgaccga agatgaacgg
     8401 gtcgccgccc ttcaggcgca ccacgcgctt gccctcgcgc gcgaggcgca ccatcagggc
     8461 gttgatgtct tcctgcggga gggcgtggtt gtcgtgctct tttccgacgt agatgcgctc
     8521 ggcgtcgcgc cttgcgagat ccaccagcgc cggcgccacc aggtggtcgt agagcaccac
     8581 gttcgcgcgc tgcatgacgc gcagcgcgcg cagcgtgagg agttccgggt cgccgggccc
     8641 ggcgcccacc aggctgacga agccgcccgc cggcggggtg ccgtcctcga gcctttgctc
     8701 cagggcgcgc gccgcctcgt cctcgcggcc ggcgagcatc agctcggcga ccgggccgtc
     8761 catgacctcc tcccagaagc gccgccgcgc ttcgggatcg ggcaggcggc gcctggacgc
     8821 ggcgcggaag cgcccggcga gcgcggcgag gcgcccgaac gcgaacggca ccgctgcctc
     8881 gatgatcgtc cgcagcttcc tcgcgaggac gggcgaggcg ccggcgctcg agatcgcgat
     8941 ctgcagcggc ggccggtcca ccaccgacgg catgatgaag ctcgagcgct ttgcatcgtc
     9001 ggcgacgttc accgggatgc gcagcgcgcg cgccgccacc gacaccgcct cgttcacttc
     9061 cgtatcgtcg ctcgccgcga tcgcgatctc cgcgcccacc agatcgccgg gcgcgaagcg
     9121 cttgggctcg tggcgtaccg cgccaagcag cgtcagctcg gtcaggcgcg ggcacagctc
     9181 gggcgccacc accgtgacgc gcacgcctga gcgcagcagc gcctcggcct tgagcgcggc
     9241 gaccgtgccg ccgcccacca cggtgccggg cttgccgtcc aggtcgagga aggcggcgaa
     9301 ataggcgcgc tcggcgacgg gggcgatgct cacgtcctcg actctaggga ccaagccggc
     9361 cgcaacattg atctaggtta aatgtgtccg gtgccgctgc ggcacccttg cggcatgcac
     9421 cggatcgcac tcgcgctcgc gctcgccgcg gcggccacca cggccttgcc ggccgatccc
     9481 gcgcccgcgc gccgcgccga gcttctgcac ctcgtgcgcc aggactgcgg ctcgtgccac
     9541 ggcctgacga tgaagggcgg cctcggcccg tcgctcgagc ccgcggcgct cgccggcaag
     9601 gatgcgaata tgctgcgctt tgtcatcctg cagggccggc acggcacggc gatgccgccc
     9661 tggcgcgccc acgtctccga ggccgaggcc ggctggatcg tggagcaact gaagaaagga
     9721 ctcccgaaat gaaacgcctc gtcagcgccg cgctcgctct ggccctcgcc gcatgcgccg
     9781 cgccgcgcgg caccggcgac ctcggcctgg tggtcgagcg cgccgcgggc cgcgtcatgc
     9841 tcatcgaaac cagcggccgc accgcgctcg ggcgcgtcac gggcctgggc gacctgtcgc
     9901 acgccgcggc cgtgttctcg cgcgacggcc gctacgccta cgtgttcggg cgcgacggcg
     9961 gcctcaccaa ggtggacctg ctgcgcgcgc ggatcgacaa gcgcatcatc caggccggca
    10021 attccatcgg cggcgccatt tcccaggacg gcagcctggt cgcggtggcc aactactcgc
    10081 ccggcggcat ccgactgttc gacgcgcgca cgctcgagcc gctcgcggag gtaccgaccg
    10141 gcgccaaggt ggtcggcatc gcggatgcgc ccggcaaccg cttcgtgttc agcctgttcg
    10201 aggcgggcga gatctggatc gtggacgcct cgcagccgcg cgcgcccgcc gtgacgcggc
    10261 tgagcaacat cggtcgccag ccctatgacg gcttcgtcac gcccaacggc cggcactaca
    10321 tcgcggggct cttcggcgag gacgggctcg cgctcgtcga cctgtggaac cccggcatgg
    10381 gcgcgcgccg catcctcgac ggctacggac gcggcgaagc gccgctgccg gtgtacaaga
    10441 tgccgcattt gcgcggctgg gcggtggccg ggcgctacat tttcctgccg gcgatcggcc
    10501 gccacgaggt gctggtgctg gacagcgtca cctggaccga ggccggccgc atcgcggtgc
    10561 acggccagcc ggtgttcgcg gtggcacagc cgggcggacg ccgcgtgtgg gtaaacttcg
    10621 cttttccgac aaacgacgtg gtgcaggtga tcgacgtgcc gcagatgcaa gtggtgcagg
    10681 agctgcggcc cgggcgcgcg gtgctgcaca tggagttcac gccgcgtggc gagcaggcct
    10741 gggtgtcctc gcgcgacgac ggcgcggtgg tggtgtacga cggcgcgagc ttcgccgagc
    10801 tggcgcgcct ccccgccgag agcccgagcg gcatctttct cacctcgcgc ggcgcgatga
    10861 ccgggttctg agatgttcga cgccgagagc tttcgcctgg tcaacgcctg gcagcggggc
    10921 tttcccctgg tgccgcggcc cttcgccgcc atcgcgaggg cgagcggctt cgacgaaggc
    10981 gagaccatcg cgcgcttcgc gcagctcaag tcgcgcgggc tgatcgatcg catcggcccg
    11041 gtgttccggc cgaatacggt cggcgccagc acgctcgcgg cgatgagcgt gccggccgag
    11101 cgtatcgagg gcgtggcgca gcaggtcagc gcccatcccg gcgtcaacca caattacgag
    11161 cgcgagcacg ccatcaacct gtggttcgtc gccaccgcac cctcggccgc ggcgctgcag
    11221 aacgtgctcg gtgccatcga agaggagacc gggctcgcgg tgctgcgcct gccgctcacc
    11281 gaggaattcc atatcgacct ggggttcgac ctcgaggacc actcggtgcc gcgcacggcc
    11341 ggggcggcgc cggtggcgcc cctgccggag gccgagcgtg cgctcgccgc gcacctgacg
    11401 gcgggattgc cgctcgtgcc gcggccctat gctgccttcg gcgttccgga aacggatctg
    11461 atcgccgcgc tcgagcgctg gctcgccacc gggatcgtgc ggcgcatcgg cgccgtggtg
    11521 cggcaccgtc cgctcggcta cgaagcgaac gcgatggtgg tgtgggatgc gcccgacgcc
    11581 gaggcgagcg acgccgggcg gcgcctggcc gccgatcccg cggtgacgct cgcctaccgg
    11641 cgcgcgcgcg ccctgcccgc gtggcccttt aacctgtact gcatggtgca cggccgcgag
    11701 cgcgcggcgg tgacgcagtc gatcgagcgc ctctcggcgc agcacggcgt gagcggctac
    11761 ccgcgcgccg tcctcttcag ccagcgttgc ttcagccagc gcgccgcgca ctatgcgtga
    11821 gctcgagcgc gccgtcgtca accgcctgca gggcggcttt ccgctcgcgc cgcgcccgtt
    11881 cgccgcggcc gcggccgagc tcggcaccga cgaggcgacg ctgatcgaga ccctgcgtgc
    11941 gatgctcgcg gcggggacgc tcacgcgctt cggcccgctg ttcgacgccg agcgcctggg
    12001 cggcgccttc acgctgtgcg ccatgagcgt gcccgcgtcg gacctcgagc gcgtcgtcgc
    12061 gcaggtgaac gcgcacccgg aagtggcgca caactacgcg cgcgagcacc gcttcaacct
    12121 ctggttcgtg ctcgccaccg gcgcgcgcgc gcgcatcgcc gaggtcatcg ccacgatcga
    12181 ggccgagacc ggctatcccg tgctcgatct gccgcgcgag cgcgagtact tcatcgacct
    12241 gaggctgacc gcatgaaagc gaccgacgaa gccatcgtgc gcgcgacgca gggcgggctg
    12301 ccgctggtgg ccgagcccta cgcggcgctc gcgcgcgagc tgggcgtcgc gcaggacgag
    12361 ctgtgcgcgc gcctcgcggc gatgctcgag gacggccgca tccggcgcat cggcgcggtg
    12421 ccgaaccact acgcgctcgg ctacaaggcg aacgccatga cggtgtggga cgtggccgac
    12481 gacgcggtgg acgagctcgg cgccgcggtc ggcgcgctcg agttcgtcac gcactgctac
    12541 cgccggccgc ggcggctgcc cgactggcgc tacaacctgt tcgcgatggt gcacggccgc
    12601 agccgcgccg agaccgaggc gcatcgcgcc gcgatccgcg cgcagctcgg cggcgcgctc
    12661 gccgccgacg acgtgctcta cagcacgcgc atcctgaaga agaccggcct gaggctcgcc
    12721 tgacatgttt cgcgtctcgc gcttcatgga agagatcctg cagccggcgc cgccggcgcg
    12781 gcgcggcagc ggcacgccgc tcggccccgt ggtgatctgg aacctgatcc ggcgctgcaa
    12841 cctgacgtgc atgcactgct actcgatctc cgcggacatc gacttccccg gcgagctttc
    12901 caccgccgag gtgcttgggg tgatggacga cctgaaggcg ttcaacgtgc gcgcgctgat
    12961 cctctcgggc ggcgagccgc tgctgcgccg cgatctgttc gagatcgcgg cgcgcggcaa
    13021 ggcgctcggc ttctacctgg ggctttcgac caacggcacg ctgatcgacg cgccgctcgc
    13081 gcggcgcatc gccgatgccg gcttcgacta cgtgggcatc agcctcgacg gcctgcaggc
    13141 gacccacgac cgcttccgcc gcaaggccgg cgccttcgac gcctcgctcg cggcgctgcg
    13201 ccggtgccgc gacctcggcg tcaaggtcgg cgtgcgcttc acgctcacgc aggacaactt
    13261 cgccgacctg ccggggctgc tcgagctggt ggaggccgag ggcatcccgc gcttttacct
    13321 ctcgcacctc aactacgcgg gccgcggcaa cgtgaaccgc aagcgcgacg cgcacttccg
    13381 cgtgacgcgc gaggcgctcg agctgctcta cgcgcgcgcc tgggacgccg cccagcgcgg
    13441 tctgcacaag gacttcgtca ccggcaacaa cgacgccgac ggcgtgtacg cgctttactg
    13501 ggcgcggcgc aatgtcccgg aattcgacga gccgcgcctg cgcgccagcc tcgccgcctg
    13561 gggcggcaac gcctccggcg tcaacgtcgc caacatcgac aacctcggca acgtgcaccc
    13621 cgacaccatg tggtgggact gcacgctggg caacgtgcgg gcgcgcccgt tctcggcgat
    13681 ctggcaggac acgcgccacc cggtgatggc ggggctgaag gagcgcccgc gccgcgtgaa
    13741 ggggcgctgc gcgggctgtg cctatttcga cgtgtgcggc ggcaacacgc gcgtgcgcgc
    13801 gatgcaggtg acgggcgatc cctgggccga ggatccgggc tgctacctcg aggacgcgga
    13861 gatcgccgcg tgagggcgct gctcgccgcc gccctgctcg cgcccgcact ggcctggccc
    13921 gccgatctgt atgagacgca ctgcgcgagc tgccacggcg ccgcgcgcct cggcgccatg
    13981 ggcccggcgc tcctgcccga gagcctcggg cgcctgaaga aggcggaggc cgcgcgcgtg
    14041 atccgcgaca gccggcccgg cgtgcagatg cccgccttct ccaagaccct gagcgaagct
    14101 gaaatagcgc agctcgtgga gctcatctac cgcccggtgg tgcccgcgcc gcgctggggc
    14161 gaagccgaga tccgcgcctc gcgcgtcgtg caccacgcgc cgggcgcgct gccggcaaag
    14221 ccgcagttcg acgccgaccc gctgaacctg ttcgtggtgg tcgaagccgg cgaccatcac
    14281 gtcacgatcc tcgacggcga ccggctcgag ccgctcgcgc gcttcgcctc gcgcttcgcg
    14341 ctgcacggcg ggccgaagtt ctcgcccgac gggcgctacg tgttcttcgc ctcgcgcgac
    14401 ggctggatca caaagtacga cctgtggaac ctgaagacgg tggccgaggt gcgcgcaggg
    14461 ctcaacacgc gcaacgccgc agtgtccgcc gacggcaagt acgtgatggc ggccaacttc
    14521 ctgccgcaca gcctcgtggt cctggacgcg gcgctggagc tggtgaaggt tctcgggacg
    14581 gactcgcgcg tctcggcggt gtacgacgcc gcgccgcgcc gcagcttcgt cgccgcgctg
    14641 aaggacgtgc ccgagctctg ggagatttcc tacgacccga aggccgagcc gatcgccggc
    14701 ggcctggtgc acgactaccg cttcaaggaa ggcgcgttcg tgccggggtt cctcaacgcg
    14761 cggcgcaccc ggctcgacca ggtgctcgat gatttcttct tcacgccgga ctttcgccag
    14821 gtggtgggcg cgagccgcgg cggcaagcgc ggccaggtgg tgcacctcga cgtgcgccgc
    14881 accatcgccg aagtggacct gccgggcctg ccgcacctcg cctcgggcat cacctgggag
    14941 cggcgcggcc ggcgcgtcat ggcgacgccc aacctgcgcg aggccgtggt gagcgtgatc
    15001 gacgtcgagg gctggaagac ggtggccgag atcccgacgc tcggtccggg ctacttcctg
    15061 cgcagccacg aagcgacgcc ctacgcctgg gtcgattcga tgatcgggcc gcagggccgc
    15121 gacacgctgc aggtgatcga caaggatacc ctcgaggtcg tggcgcgcct cacgcccgcg
    15181 ccgggaaaga cgctcgcgca cgtcgagttc gaccgccacg gccgctacgc gctcgccagc
    15241 ctgtgggagg ccgagggcgc gttgatcgtg ttcgacgcgc gcacgctcgc cgaggtgaag
    15301 cgcatcccga tgcgaaagcc cgtgggcaag tacaacgtgc acaacaagat cacgcgctcc
    15361 gagggcacca gctggtgaac cggcgcgcgc cgcagatcaa gatccaggcg ttcctcgcca
    15421 acctgccgat gttcggcgag atgagcgcgg cggagctcga ccgcatcgcg gcgagcacct
    15481 tgccgctgca cgtcgacaag ggcgagaccg tgtgccacac gggcgatccg tgcacgggct
    15541 ttcacgtcgt ggtctacggc caggtcaagc tcggcttcac ctcgccacag ggcgtggaga
    15601 aggtggtcga gatcgtgcgc ccgggccaaa gcttcggcga ggcgctgatg ttcctcgacg
    15661 ccccctacat cgtgttcgcg caggcgctcg ccgactccat gctgctgcac gtcgccaagc
    15721 acgcggtgct cgacgagctc gggcgcgacc cgggcttcgc gcggcgcatg ctctcgggcc
    15781 tctcgcagcg gctgcacggg ctggtgcgcg acgtcgaggc ctacagcctg cgctcgagcc
    15841 acgagcgcgt gatcggctac ctgctcgccg aagcgcccgt agacgccaac ggcgcgccgg
    15901 tcgaagtgca gctctcggcg ggcaagagcg tcatcgcgtc gcgcctcaac atgacgccgg
    15961 agcatttctc gcgcatcctg cacgagctcg gcaacgacgg cgtgatcgag atccgcggcc
    16021 gcaccgtgcg catcccgaat ctcgcgcgcc tgcgcggcag tgcgtagcac gccggcgcag
    16081 gatcgttgat gcgcgtcaaa ccgctcctcg gtcggtgagc ccggcgcccc tagaatcgcg
    16141 cgggtggaac tggtctgtcc cgccggcgcg ctgccggccc tgaaagcggc cgtcgacaac
    16201 ggcgccgact gggtctacct cggcctgcgc gacgacacca acgcgcgcaa tttccctggc
    16261 ctcaatttcg acgagtccgc gctcgtcgcg gggctcgcct acgcgcatgc gcgcggccgc
    16321 aaggtgctga tggcggtcaa cacctacccg caggcgggca gctgggcgcg ctggaccgcc
    16381 gcggtggatc gcgcggcacg gctcggcgtg gacgccatca tcgtcgccga cgccggcctg
    16441 atgcgctatg cgagccgtac gcacccgcgg ctggagctgc acctctcggt gcaggcctcg
    16501 gcgaccagct acgaggcgat caacttcttt cacgagcatt ttggcgtcaa gcgcgcggtg
    16561 ctgccgcgcg tgctgtcgct cgcccaggtc gcgcaggtcg cgcgccatac gccggtcgag
    16621 atcgaggtct tcggctttgg cagcctgtgc gtgatggtcg aggggcgctg cacgctctcg
    16681 gcctacgcct gcggcgaggc gcccaatacc accggcgcct gctcgcccgc gcacgcggtg
    16741 cgctggcgcg aaaccgccgc cgggctcgag tcgcggctga acggcatcct catcgaccgc
    16801 tacgcgccgg gagagcgtgc ggcgtatccc accgtgtgca agggccgcta cgcagccacc
    16861 ggcgagacct actatgcctt cgaagagccg gcgagcctga acacgctcga tctcgtgccg
    16921 cagttcggcg aggccggcgt gcgcgcggtg aagatcgagg ggcgccagcg cagcccggcg
    16981 tatgtggcgc aggtgacgcg cgtctggcgc gaggcgatcg acgcctggaa ggcgggcggg
    17041 ttcgcgccgc gcccggcctg ggccgcggcg ctccagcgcc acgccgaagg cagccacacc
    17101 accctcggcg cgttttcgcg accctggaaa tgaagctcgc gctcggcccg ctcctttact
    17161 actggccgcg cgacgcagtg ctcgccttct acgaggagat cgctgcggcg cccgtggaca
    17221 ccgtgtacct gggcgaggtg gtgtgctcgc ggcgccacga gctcgcgttc gaggactggc
    17281 tcgcgattgc cgcgcggctc gccgcggccg gcaaggaggt ggtgctctcg gcgcaggcgc
    17341 tcacggagtc cgagggcgac ttgaagcttg tgcgccgcgc cgtggccaac ggccgctttc
    17401 gcgtggaggc gaacgactgg ggcgcagtgc gcctgctcgc cgccgtcacc ggttggatcg
    17461 ccggcccgca cctcaacgtc tacaacccgc aaacgctcga cttggtgacg gcgctcggcg
    17521 cgacgcgctg ggtcgcgccc acggaggcga cgcgcgacat ggtcgccggc ctcctcgccg
    17581 agcggccggc gggactggag gccgaggtgt tcgcctacgg tcgcatgccc ctcgcctttt
    17641 ccgcgcgctg cttcacggcg cggcggctga accgccagaa ggaggactgc ggctacgcct
    17701 gcctcgattt cccggacggc atcgcgctca agactcgcga gggcgagccg tttctcgcgg
    17761 tgaacggcat tcagacgctc tcggccggcg tatattgcct cgtggacgag cttgcggcgc
    17821 tgcgcgagac gggggtggaa gtccttcgcg tcagccccca gtcggtcggc acccacgacg
    17881 tactgcacat cctgcgcgca gcctgcgacg acgcgctaag cggtttcgac gcgcgcaagg
    17941 cgctcgcggc cgtggcgccc ggcaagctct ggaacggctt ctggcacggg cagcccggga
    18001 tgccggcgcg gtgatcccgc tctccgaccg gctgcactgc atgctcgcgc cgctgcgcgg
    18061 caaggtgctg cgcctggaaa tcgccggtct cggcggcggc ccgcagttca cgctggattt
    18121 catcggcctg cgcccgtcct tcggcaagcc cgacgtgacg gtgcgtgcaa gcctcggcga
    18181 ttaccttgcg ctggcgttgc gtcgcgaaga tcccgacacg ctcttcttca cccgccgcct
    18241 ggtgatcgaa ggcgacaccg aactcgggct gcagatcaag aacgcgctcg acgcgctcgg
    18301 ctgaccaaaa aaaagggcgg gagcaacgct cccgccctcc gctgcgtccg gaccgttcag
    18361 cccatcttca ggatccagtc gaccagcgtc tttatgtcgg catcgggcac cgccgggttg
    18421 ggcggcatgg gaaccggccc aaacgcgccg ctgccgccct tcttgacctt gtccgcgagc
    18481 atccccgcgg cactggcctg ccccttgtac ttcttggcaa tgtccttgta ggaggggccg
    18541 accagcttct tgtcgggctg gtggcaggca aggcagttgt gcttcttggc gagcgcctcg
    18601 tcggcgcgcg ccggcgtgct cagtaccgcc gcggccgcgg tcaaggcaac gatcacgatc
    18661 ttcatgttcc ggacgtcctt tctagtagat gtcgtgctgc gtattgtaaa cgttgaagtg
    18721 cccggtcggc gtgatcagcc gcggatcctt gatcacggcc ttcagcttcc tggtcttgtc
    18781 gtcgaccacc accagcgccg actcctggtt cttcgcgctc cacaccgaga accatacctc
    18841 ggtgcccgcc ttattgtact cgggctgcac gatgcgcttg gcgccctcgc ccaggttcgc
    18901 ccattcgccg atcggcagga ccacggcagg cttgccgagg ttcccgatgt cgaacacggc
    18961 gaccgactgc gagatcttcg ggtcggggtt gagcggcgtg tcgacccaca ggttcttcga
    19021 cttcgggtgg gtcttgatga agagattgcc gccgccctgg ccgtcgagca tctgcaccac
    19081 cttccaggcg tgctgcttgt tcttcgccgg gtcggtgccg atgagcgcga tcttctcgct
    19141 gccgaggtgc ccggtggccc agaccgggcc gtacttcggg tgcacgaagt tcgcgccgcg
    19201 ccccgggtgc gggatcttgt cgacgtcgat caacgccgcc agcttgtccg tcttggtatc
    19261 cagcaccgcg atcttgttcg actggttggc ggcgacgagg aagtagcgct tgctcgcgtc
    19321 ccagccgccg tcgtgcagga accgcgccgc ctcgacttcg gtcgacgtga tcgccttgag
    19381 atcggtgtag tcgaccatca cgatcttgcc ggtttccttg acgttgacca cgaaatgcgg
    19441 tcgccggtgc tcggccacga tcgcggcaac gcgcggctcg ggatggtatt cctgcgtgtc
    19501 gaccgtcatg ccgcgggtgg agacgatctt cagcggctcc agcgtatcgc ccttcatgat
    19561 cacgtactgc ggcggccagt aggcgccggc gatggccagc ttgtcctcat agcccttggc
    19621 cttcgaggta tccaccgagc gcgcctccag gccgatgcgg atctccgcca cgttgtcggg
    19681 cttctccatc cacagatcga tcatgttgat cttcgcgtcg cggccgatga ccaagacata
    19741 gcggccggag gctgacacgc gcgagatgtg caccgcgtag ccagtcttca cgatgttgat
    19801 gatcttcttg gtgtcgccgt cgatcagcgc cacctcgccc gtgtcgcgca gcgtcgtcga
    19861 gaagatgttg gcgatgttga acttgttcat cttcttcttc ggtcgctgcg cgggcggcac
    19921 gatgaccttc caggtcttct tcatgtcggc catgccgaat tccggcgggg tcggcggcgt
    19981 ctgctgcacg tagcgcgcca tcaggtcgac ctcgtcgtcg gacatgtcgc ccgaggtgcc
    20041 ccagttcggc atgcccgcgg gcgagccgta cttgatgaac actttcaggt actcggtgcc
    20101 cttgggcagc gtgatgtcgg gcgtgagcgg cttgccggtg gcgcccttgc gcagcacgcc
    20161 gtggcagccg gcgcagcgct cgaagtacag cacgcgcgcg cggtcgaact ccgccttgct
    20221 catcggcggc gccttcgggt tcacgtcctg gtgcatgtcc tccgccgcga gcggcgagcc
    20281 gcccgcctgg taccggatct cggattcggt ggtcggatgc ttggcggcct gtccgtaggc
    20341 tgtcgcagcg gcgagcggca gcaagagcca cgccgcagca cgcgtcaggc ggatgttggg
    20401 attcgtcacg agtacctccc tgtgctgatt gaaatcgtca ttcccgctag ggtgcgccgc
    20461 agcgcgcccg aaaactttga tttatctcaa gtgcttggct cgatcagcag ctcgaacgcg
    20521 ccgtcggcgc gctgcgcacc gctgtaggag aagccgcgct cgcgtagcag agggtacaac
    20581 ggcagcggct cgtgcggcag gatcgcgcgc agcgactcgc cgggcgcgcg ctccagcgcg
    20641 gcgaagatgc gcacgatcgg ctcgggcggc tgcaagccgc gcaggtccag cgttggggag
    20701 tccgccggcg cccgccctgc gcgcagcgcg tcggcgtgcc tctcgcgtag cagcgcggca
    20761 agctcctcga aagcggcgcg gcagccctcg gggtcgtgct gcggcgccgc ggcgcctagg
    20821 gtatcgaagc gctggcacag gtcctcgtcg cgctcgagca gcgcggcgca ggcctggcgc
    20881 agttgcgcga agcgcgcgcg atgcgtcgcc cagtcgcccg cacgcgccgc ggcgagcgca
    20941 gcctcgatca acccatcggc ggactgcacc atggcgcgac cttcgcaagt cgcgccaccg
    21001 ccgcccttga tgtgggtcaa gtaaggcggg cgtgcgcgta ggcggcggcg gcggcgagca
    21061 gcgcgccggc cgccccgtgc gcgagcgacg cggcgagcgg ctggccgaac agcgccgccg
    21121 cggcgccggc ggcgacgacc gcgagcgcga gcccgagcag cgcgaagcgc tgcgccgcac
    21181 gctccagccg cagcgcgagc cacgccgccc cggccgcgag ccccagcccg agcgccacgt
    21241 gcccggcaag cacccagccc cagatgtcct cggcgaagat cgcgagccaa gcaccgagcg
    21301 cgcattgcgc cacgaacagc gcggcgaccg cgtgtccgag ccggcgtgct gcccgcgccg
    21361 cgtggccttg cgcgcgcccg agcagccagg cgaacgccgc ggcaagcgcg agcccgccca
    21421 gcaagttggc ggcggcagcc gcgggcgggg gattctgccc cacggcgatt ccgagcaccg
    21481 acaagaacac cgtgagcacc gccaccccga gcgcccggcg ccaggcgagc caggtgagtg
    21541 ccagggccgc caccacttcg agcgaggcgg cggcgcgatg cacgccgcgc accgcgccga
    21601 gcacggcgcc gagctcgtcg ctcgcatggc ggatggcgag gctggcgaag agcacgacca
    21661 gcaccaggcc gaggcccgcg gcgctccacg cgctggtcac gcgtgccggt gcgcgttcca
    21721 tatcgccgcg gccagcacca gcaccgcgcc gccctggtgc gcggcgccga gcgccaccgg
    21781 cacgccggcg agcagcgtgg cgatgccgag cgtggcctgt agcacgaagg cgccgagcag
    21841 cgcgtgggcc gcccagtgcg tgcgccgttg ccaccatgcg atcggcacca gcaccgcgag
    21901 cagccagaac aagccgcggt gcacgaattg cacggtggcc atgttgtaga ggaaattccg
    21961 ccaccacggc tcgagcatca ggatctcggg cggcacccag tgcccgttca tgagcggaaa
    22021 cgtgttgtag gccgcgcccg cgcgcagccc cgccaccatg ccgccggaca gcgccatgac
    22081 gaacactgcg aaaggcagcg ccgcggcaag gcgcggcgcg cggcccgcgc gcggccagag
    22141 cagcccgagc gcgatccaca gctccgcact gaagatggcg agcgcgagcc cgaggtgcag
    22201 ggtgaggcgc accgggttca ccttcgggtc gtcgatcagg ccggaggcca ccatcagcca
    22261 gcccatcagc ccctgcagcg cgccgagcgc gaagatgccc cagagcttcc aggcgagccc
    22321 cggctcgagc ttctttttca gctggaacca gataagcggc agcagaaaca ccaggccgat
    22381 ggcgcgcgcg agcaggcggt gcacgtactc ccaccagaag atgcgcttga agccctccag
    22441 atccatggcg aagttgacca gccggaattc cggcgtctcg cggtacttgg cgaacagctc
    22501 cgcccagtgc gcctccgtga gcggcggcag cacgccgatg agcggctgcc actcgacgat
    22561 ggaaagcccc gagcgcgtca ggcgcgtgac gcccccgacc agcacgatgg cgaacaccat
    22621 tgcagcgcac aacagcagcc aggccgcgac ctggcggcgc gcggagtcgg tcatgacggc
    22681 gagtgtgccc ggcttcggtc cggaaagctt gataggtatc aaatttttgc gcccacaggg
    22741 ctggtctact agccgctgtc tgaaccagcg aaggggacga gatgaacgcc gaacgcattg
    22801 aacacgacga agtgcccgac gagccggtcc cggcgatgca acagctgctc gacaacccgt
    22861 tcctgctgct gtttctcggc atcgccatgc ccaccgtgct ctacatcgtc tggggcgtga
    22921 tggaaatcgt gtccctgccg atcgccaaat aagggagcac acatgagcgc aatcctgccc
    22981 ccgcaggacc gagtatggtg gaagcagccg ctcgaccgcg tcgagctgct gtggatcgtg
    23041 gtggcgctga tctggtgcct gatcatgttc ttcatgatgc cctactggca cgtctacggt
    23101 cagcagaacc tttccaacga ggcctaccgc tcgaagcccg aggacttcgt caagaagacc
    23161 gaggcgatga tcgcgcaata caaggtgcgc gaggacggcg gcgacctcaa ggtgccggtg
    23221 gtgcgcccgc ccgccggcag cgacgtctac ctggtggcgc agctgtggcg ctggtacccg
    23281 atcctcgagc tggaagcggg caagtcctac cgcatccacc tcatgtcgct cgactggctg
    23341 cacggcttct cgctgcagcc cgagaacatc aacatccagg tgcaccccgg ctacgagcac
    23401 gtgctcaccg tgacgcccac caacccgggc acctactcga tcatctgcaa cgagtactgc
    23461 ggcatcaacc atcacacgat ggtgagccgc ctgtacgtgg tgaagtaagg gagaccgccc
    23521 atgagcgtca accggatgtt ccgcacctgc ccgtcgagcg gactggtatt ccacgagccc
    23581 gccgagaagc tgatgcgctg gaatgccgtc gcggccgtgg tgtcgctgct gatcgggggc
    23641 ctgctcgcca tcggcgtcgt gctcacccgc tggcaggcgg tgcacctgct gccgcccgac
    23701 cgcttctacc agtacctcac cgcccacggg ctcaacatgc tcgtgttctg gatcatcttc
    23761 ttcgagatcg cggtgcttta cttcgccagc tcgacgctgc tcaagtcgcg catcgcgacg
    23821 ccagccatgg cgtggctcgg cttctggctg atggtgctcg gcgcgctggt caacaactac
    23881 tcggtgttcc gcggcgattc cagcgtcatg ttcacctcct acgcgccgat gggtgcgcac
    23941 cccacgttct acctggggct gatcttgttc gcggtggggg cgctcatcgc ctgcttcgtg
    24001 ttcttcggca ccctggtggt ggcgaaggcc gagcgcacct accacggctc ggtgccgctg
    24061 gtgaccttcg gcgcgatcac cgcgggcatc atcgccgtgt tcacgatcgc cgccggcgcc
    24121 atcatcctga tcccgacgtt cctgtggtcg gtggggctgg tcgagcacat cgattcgctc
    24181 atgtaccgca ccgtgtggtg ggcgttcggc cactcctcgc agcagatcaa cgtcagcgcg
    24241 cacgtcgccg tgtggtacgc ggtggcggcg atcgtgttcg gcgccaagcc gctctcggag
    24301 aaggtaagcc gcaccgcgtt cctgctgtat atcctgttcc tgcagctggc gagcgcgcac
    24361 cacctgctcg ccgaccctgg cctcacctcg cactggaaga tcttcaacac gagctacgcc
    24421 atgtacctcg ccgtgctcgc ctccatggtg cacggactga cggtgccggg gtcgatcgag
    24481 gtggcgcagc gcgaaaaggg gttcaaccac ggcctcttcg agtggctgag gaaggcacct
    24541 tggggcaacc cggcgttctc ctcgatgttc atgtcgctga tcggcttcgg cttcctcggc
    24601 ggcatctcgg gcgtgatgat gggcaccgag cagttgaacc tgatcatcca caacacgctg
    24661 tacgtgccgg ggcacttcca tgccacggtg gtcgtgggca cgacgctcgc cttcatgggg
    24721 ctcacctact tcctgatccc ggtgctgttc cgccgcgagc tcgccttccc gggcatggcg
    24781 aagtggcagc cgtacgtgtt cggcctgtcg atgatgggcg tgtcgctgtt cctgatgggc
    24841 gcgggcacgc tcggcgtgcc gcgccggcat tgggacatcg gcttcgccgg caacgcgttc
    24901 gcgcatgagt tccccggcgc cgcgtggctg atgatggcgc tcgccggcat caccggcctc
    24961 atcgcgatcg tcggcggcgg catgtacctg ctgatcacgg tgtactcggt gttcctcggc
    25021 aagaagatcg agacgccggg cttccagcac gtgcgcgcga aggacggcat cgcgccggcg
    25081 cccaagccgg tggcggcggc gatcgcgggg caccacccga acgtgggcgc gggcggcctg
    25141 gtggcgccgg gcaccttcgc cctggcgatg gtgttcctcg cctccttcgt cctgtactac
    25201 ttcatcaact ggaagtacct gtcgacggtc tggccgctga agtgagacag gttctcaatc
    25261 tcttcaagct caggatcggc gtcgccatcg ccgtcgccgc cgccgccggc atggcggtgg
    25321 agtccggcgc cggccccggt gccgccgccg gtgcgatgct gatcctcgcg gtgctggtcg
    25381 cctcggcagc cgccggcgcg ttcaaccagt acgtggagcg cgatctcgac gcgcgcatgg
    25441 gccgcacgag gaaccgcccg ttcgtcaccg gggcgctgaa gcacgggccg gcgtggctgt
    25501 ggctgatcgg cgcgatgctc gcgctttcgg tgctcgccgc cgccgtggcg gcgaacccgc
    25561 tcgccgcgct gtacgtgttc ctcggcgcgt tcacctacgg tgtggtctac acggtgtggc
    25621 tgaagcggcg cacgtggtgg aacatcgtgg tcgggggact cgccgggagc ttcgcggtgc
    25681 tcgccggcgc ggccgcggtg tcgcccgcac caggccccga ggcgctcgcg ctcgccgtgg
    25741 tgctctttct gtggacgccg ccgcatttct ggagcctcgc catcgtctgc cgcgaggatt
    25801 acgcgaaggc gcgcgtgccg atgctgccgg tggtggtggg cgacgcgcgc gcggcgcgtg
    25861 ccatcttcgc cagcgcagcg ctcctggtcg gcgcctcgtt ggtgcctgcg gccttcggcc
    25921 tgggctggat ctacctctcg tttgccgcag ccggcgggat tcttttcctg ctgcgctgcc
    25981 gggcgctcgt gcgccgcccc ggacgcggcg ccgcgcgcgc cgcgttcctc gcctcgctct
    26041 tgcagctggg cgcggtgctc gcgggcgcca tgctcgatgc tgcgcttctc gcttagcgcg
    26101 ctgctgctcg ccgcggcgct cggcgcgcac gccttcgacg agaaactcgc gctgcgcgag
    26161 agccaggccg cgatcggccg cccgatcggc gactacacgc tgcgcgacac cgaggggcgc
    26221 accgtgcggc tcgccgagct gcgcggcaag ccgctggtgg tgaacttcgt gtacaccggc
    26281 tgcttccaga tctgcccggc gagcacgcag ttcctcgcca aggcgatcgc cgaggccgag
    26341 cgcacgctcg ggccggggac gtttcgcgtc gccaccatcg gcttcaacct gccgttcgac
    26401 accccgggcg cgatgaagga gttcgcgcgc aagcacgcgg tcgaatcgcc ccactggctg
    26461 ttcctgtcgc cggaggcgga gacgctgccc gcgctggtgt cggaattcgg ctttcgctac
    26521 gagtccacca cggccgggtt cgaccac
//
LOCUS       DBNHKE010000223         7256 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_624207, whole genome shotgun sequence.
ACCESSION   DBNHKE010000223 DBNHKE010000000
VERSION     DBNHKE010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_624207"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..927)
                     /locus_tag="AC16O9_15105"
     CDS             complement(307..927)
                     /locus_tag="AC16O9_15105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6517982.1"
                     /translation="MIDLYTWSTPNGRKVSIMLEECALPYKVHPINIGKGEQFAPEFL
                     ALNPNNRIPAIVDSDGPDGKPLPLFESGAILIYLAEKTGKFIPKDARTRLICLQWLMF
                     QMGGVGPMFGQAHHFIRNKEVVPYGIKRYSEETRRLYGVLQKRLSEAEYLADEYSIAD
                     IATFPWVARFEWHKVDLADFPAVKRWFDKINARPAVQKGMAVPTPP"
     gene            972..1166
                     /locus_tag="AC16O9_15110"
     CDS             972..1166
                     /locus_tag="AC16O9_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR6517983.1"
                     /translation="MLKWLVTILLSLAVLAAATPWLQRLGIGRLPGDLKFSLRGRAYY
                     VPFASTVLFCLVAWVIGRVL"
     gene            1211..2488
                     /locus_tag="AC16O9_15115"
     CDS             1211..2488
                     /locus_tag="AC16O9_15115"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6517984.1"
                     /translation="MKKILVLFPKEWDRLELARPEYRERYEFVFAGFDLFRFPQNAQL
                     ATFDVFGFVNRLLARFRHERLDGVFSNNEYFGALIAAVLAEKLGLPGTDPRVVITAQH
                     KFYARQAFARIAPEAGARYAAFPYTVRSRADLPFELPCFVKPVRATYSVLARRIERFD
                     ELQRHLSFWPFEKWVIKRLVKPFNDLMRWYTDYALDAHHLIAEEILPGVQVNMDGYVH
                     DGCVRALGVIDEHMYPGTCAFRSFEYPSRLPAEVQARMTALAGKLLTGLGYRHGFFNL
                     EFCWDPETGRIAVLELNPRMASQLSYLYECVDGLRPYEMLLALATGESPEGAPPTPSY
                     RNAASFALRKFDGKPLAAHPTPEQLARVRHEFPEAHLMLYLKRGASLAREMKWLGSYR
                     YAVVNMAAGSQAALYERFVALTRRLFPASDLPA"
     gene            complement(2471..3052)
                     /locus_tag="AC16O9_15120"
     CDS             complement(2471..3052)
                     /locus_tag="AC16O9_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6517985.1"
                     /translation="MDTEHRILAAAARLFAERGYAGARTAEIARAARVTERTLFKHFP
                     DKDALLERVLAPALALAATPPPASAASFAVWFETLIRQRLAAAQAHPHALRLVLVELL
                     TDEDARRRFAPLWKRELWGGLANAVARFQARGELRADLEAEALARMVLSLALGYLLSR
                     TLVAPGLAWDDAREIGRMLAVLRQGAAGSGGQV"
     gene            complement(3076..4134)
                     /locus_tag="AC16O9_15125"
     CDS             complement(3076..4134)
                     /locus_tag="AC16O9_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR6517986.1"
                     /translation="MKKYVVGGVVRDELLGLPVADRDWVVVGATPEDMVRQGFRPVGK
                     DFPVFLHPETHEEYALARTERKSGRGYRGFTVYAAPDVTLEADLARRDLTINAMARDE
                     AGRLIDPYGGEKDLRAGVLRHVSEAFEEDPVRILRVARFVARLGFRIAPETQALMRRM
                     VASGEADHLVAERVWQEFSRGLMETHPARMLDVLAECGLAARLLPELKNERAALERAA
                     AAGAPLAVRFAVLTWGLEEDAVRALCERLRAPNEDLELAQAASRCRPLLAARAPRGLL
                     ELLKRADAVRRPERFARLLEAVRLAEPGADLQRVEKAFAAAAAVDAGAIARESSADIG
                     ARVEQAREAAIGKALAQM"
     gene            complement(4131..4268)
                     /locus_tag="AC16O9_15130"
     CDS             complement(4131..4268)
                     /locus_tag="AC16O9_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6517987.1"
                     /translation="MLHVVGNKNHSSWPLRPWVAMKVLAVLGYLAEREAESLRQLEQY
                     A"
     gene            complement(4277..5203)
                     /locus_tag="AC16O9_15135"
     CDS             complement(4277..5203)
                     /locus_tag="AC16O9_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR6517988.1"
                     /translation="MRHEDVLVVGGSGFVGRHLVAALAKRGARVTVPTRRRERAKHLL
                     LLPTVEVVEADVGRAGELERLAAGKDTVVNLVGVLHSRRGRRDERGPNDYGPDFARAH
                     VELPQAVVRACRAAGAKRLLHMSALGADPQAPSEYLRSKGIGEQAALAADDLAVTVFQ
                     PSVIFGPEDGFLNLFAQLARFLPVLALACPEARFQPVYVADVAQAFVAALDAREAVGR
                     RYTLCGPRQYALRELVQAVCRITGRRRLVIGLGDRLSYWQARLMELAPVKLMTRDNYY
                     SMQVPSVCDGVFPFGIEPAALEGVAPGWLAPR"
     gene            complement(5206..7062)
                     /locus_tag="AC16O9_15140"
     CDS             complement(5206..7062)
                     /locus_tag="AC16O9_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6517989.1"
                     /translation="MLGAYDAFRAGDPDRFERHARALEDHTLAPWVEYWRLKLRIEDA
                     DAADIERYLAGHANTYLGDTLRADWLKELGRRGDWPAFERALGPLVQDELEIRCHAWR
                     ARLARGDVPAEIEARALWSAELRELPEGCRKLVGELVARNAVDEERIWQRARLQLYYG
                     YVTAARRTLEDLPAAARPDERLLAQAVTAPRQLAARGPRDLEPRATREMLVFALLRLA
                     RSDLDAAARALREGAGARLPPAEREYLWARLATLAAFEHRDEALDWYAHAGAAPLDDT
                     QLAWKARAALRAGRWQAVLDAIEPMSPQAGLDLAWTYWRGRARDALGDAEGAREQYLR
                     IAGQPLYYSVLATEELGGEASIPMPYHEASEAEVDAARSHPELARALELYRLGLRAEA
                     TREWMFAVRHMGDRRLLAAAELARRAAVFDRAIGTAGRTELLHDYRMRYLAPYREVFR
                     EYAGAYGLEEAWVLGTVRQESRFIPEARSGAGATGLMQILPHTARWVARRIGYKDYSP
                     KRVAEVETNVTLGARYLQHMLERTGHPVLASTAYNAGPSRALRWQPARPLEGAIFIES
                     IPFDETREYVKRVMANTVHYALLLEGRSAPLKARLGTITPRSAGAAAAEVTD"
BASE COUNT         1049 a         2662 c         2481 g         1064 t
ORIGIN      
        1 gcgcatgcag gagaacatgg cctggatgaa ccgcgtggta ggcacgcagc cgatccccgg
       61 cgagccgcag ctgcgcatcg acgagatcaa cgccttcctg gcgaagtacg cgagaaagaa
      121 ttagggacag acctcaatta cgcgacgacg cacgcgtcat cgcgacgctc ttgcccttca
      181 atacatctcg gcttttccgg cggggtgaat cttcccgccg gaaaagccga gaccatatgg
      241 aacccaacgc aggcttcggt gctatcgctt cgcttgcgcg agaaactaaa tgaggtctgt
      301 ccccatttac gggggcgtcg gcaccgccat gcccttctgc accgcaggcc gggcgttgat
      361 cttgtcgaac cagcgcttca ccgccgggaa gtcggcgagg tccactttgt gccactcgaa
      421 gcgcgccacc cacggaaagg tggcgatgtc ggcgatcgag tactcgtcgg cgaggtactc
      481 ggcttccgaa aggcgcttct gcagcacgcc gtacaggcgc cgcgtctcct cgctgtagcg
      541 cttgatgccg tagggcacca cttccttgtt gcggatgaag tggtgcgcct ggccgaacat
      601 cgggccgacg ccgcccatct ggaacatgag ccactgcagg cagatgagcc gggtgcgcgc
      661 gtccttcggg atgaacttgc cggtcttctc cgccaggtag atgaggatcg cgcccgattc
      721 gaacagcggc agcggcttgc cgtccgggcc gtcggaatca acgatcgccg ggatccggtt
      781 attggggtta agggcgagaa actcgggcgc gaactgctcg cccttgccga tgttgatcgg
      841 gtgcaccttg tagggcagcg cgcattcctc cagcatgatg gagaccttgc ggccgttcgg
      901 cgtgctccac gtgtacagat cgatcatgcg cggctccggg caaagcgcgc gagtgtatcc
      961 tgtcggggcc gatgctcaag tggctggtca cgatcttgct gtcgctcgcc gtgctcgcgg
     1021 ccgccacgcc ctggctgcag cgcctgggca tcggccggct ccccggcgac ctgaagttca
     1081 gcttgcgcgg ccgcgcgtac tacgtgccgt tcgcgagcac cgtgctcttt tgcctcgtgg
     1141 cgtgggttat cggccgggtc ctgtgaaccg taaggaatag ccggcgcccg gcgactgttt
     1201 taccgaacag atgaagaaga tcctcgttct cttcccgaag gagtgggacc ggctcgagct
     1261 ggcccggccg gagtaccggg aacgctacga gttcgtgttc gcgggcttcg acctgttccg
     1321 ctttccgcag aacgcccagc tcgccacgtt cgacgtgttc ggcttcgtca accggctgct
     1381 cgcgcgcttt cgccacgagc gcctggacgg ggtgttcagc aacaacgagt acttcggcgc
     1441 gctgatcgcc gcggtgctgg ccgaaaagct cgggctcccc ggcaccgacc cgcgcgtggt
     1501 gatcaccgcc cagcacaagt tctacgcgcg ccaggccttc gcgcgcatcg cgccggaagc
     1561 cggggcgcgt tacgccgcgt tcccctacac ggtgcgcagc cgcgccgacc tgcccttcga
     1621 gctgccctgc ttcgtgaagc ccgtgcgcgc cacctactcg gtgctggcgc ggcgcatcga
     1681 gcgcttcgac gagctgcagc gccacctgtc gttctggccg ttcgagaagt gggtgatcaa
     1741 gcgcctggtg aagccgttca acgacctgat gcgctggtac accgactacg cgctcgacgc
     1801 gcaccacctg atcgccgagg agatcctgcc cggcgtgcag gtgaacatgg acggttacgt
     1861 gcacgacggg tgcgtgcgcg cgctcggggt gatcgacgag cacatgtacc cgggcacctg
     1921 cgcctttcgc agcttcgagt atccctcgcg cctgccggcg gaggtccagg cgcgcatgac
     1981 ggcgctcgcc ggaaagctgc taacgggcct gggctatcgc cacggcttct tcaacctcga
     2041 gttctgctgg gatccggaga ccggccgcat cgcggtgctc gagctcaacc cgcgcatggc
     2101 ctcgcagctt tcgtaccttt acgagtgcgt cgacggcctg cggccctacg agatgctgct
     2161 cgcgctcgcg acgggcgaat cgcccgaggg cgcgccgccc accccctcct accgcaatgc
     2221 ggcaagcttc gcgctgcgca agttcgacgg caagccgctg gccgcgcacc ccacgcccga
     2281 gcagctggcg cgcgtgcgac acgagtttcc cgaagcccac ctgatgctgt acctgaagcg
     2341 cggcgcgagc ctcgcgcgcg agatgaagtg gctcggcagc taccgctatg ccgtggtcaa
     2401 catggccgcg ggttcccagg ccgcgctgta cgagcggttt gtcgcgctca cgcgcaggct
     2461 gtttcccgcc tcagacctgc ccgcctgatc cggccgcgcc ctgccggagc accgccagca
     2521 tgcgcccgat ctccctggcg tcgtcccagg cgagccccgg cgccaccagc gttcgcgaca
     2581 gcaggtagcc gagcgcaagc gacagcacca tgcgcgcgag cgcctcggcc tcgaggtcgg
     2641 cgcgcagctc gccgcgcgcc tggaagcgcg cgaccgcgtt cgccagcccg ccccagagct
     2701 cgcgcttcca gagcggcgcg aagcgccggc gcgcgtcctc gtccgtcagc agctcgacca
     2761 gcaccaggcg cagggcgtgc gggtgcgcct gtgccgcagc cagccgctgg cggatcagcg
     2821 tctcgaacca gacggcaaag ctcgccgcgc tggccggggg cggcgtggcc gccagagcga
     2881 gcgccggcgc cagcacgcgc tcgagcagcg cgtccttgtc ggggaagtgc ttgaacagcg
     2941 tgcgctcggt aaccctggcc gcgcgcgcaa tctccgcagt gcgcgcgccg gcatagccgc
     3001 gctcggcgaa caggcgcgcc gcggccgcca gaatccggtg ttcagtatcc actataccta
     3061 agtatagtag acactttaca tctgcgccag ggctttcccg atagccgctt cgcgtgcctg
     3121 ctccacccgg gcgccgatgt cggcgctcga ctcgcgggcg atggcgcccg catccaccgc
     3181 ggcggccgcg gcgaacgctt tctccacgcg ctgcaagtcc gccccgggct cggccaggcg
     3241 cacggcttcg agcagccgcg cgaagcgctc gggccgccgc acggcgtcgg cgcgcttcag
     3301 cagctcgagc agcccgcgcg gggcgcgcgc cgcgagcagc ggccggcagc ggctcgccgc
     3361 ctgcgcgagc tcgaggtcct cgttcggcgc ccgcaggcgc tcgcacagcg cgcgcaccgc
     3421 gtcttcctcc aggccccagg tgagcaccgc gaagcgcacc gcgagcggcg cgcccgcggc
     3481 cgccgcgcgc tcgagcgccg cgcgctcgtt cttcagttcc ggaagcagcc gcgcggcgag
     3541 gccgcactcg gcgagcacat cgagcatgcg tgccggatgc gtctccatga ggccgcgcga
     3601 gaactcctgc cacacgcgct cggcgaccag gtggtcggct tcacccgagg cgaccatgcg
     3661 ccgcatcagc gcctgggtct ccggcgcgat gcgaaagcca aggcgcgcga cgaaccgcgc
     3721 cacgcgcagg atgcgcaccg ggtcttcctc gaacgcctcg ctcacgtgcc tgagcacgcc
     3781 cgcgcgcagg tccttctcgc cgccgtaggg gtcgatcagg cgcccggctt cgtcccgcgc
     3841 catggcgttg atggtgaggt cgcgccgcgc caggtcggct tccagcgtca cgtcgggcgc
     3901 ggcgtacacc gtgaatcccc tgtagccgcg cccgctcttt ctctcggtgc gcgcgagcgc
     3961 gtactcctcg tgcgtctcgg ggtgcaggaa caccgggaag tccttgccca cgggccggaa
     4021 gccctggcgc accatgtcct cgggcgtggc gccgaccacc acccagtcgc ggtcggcgac
     4081 cggcaggccg agcagctcat cgcgcacgac cccgccgacg acgtactttt tcacgcgtac
     4141 tgttcgagct ggcgcagcga ttcggcttcg cgctcggcga gatacccgag gacggcgagc
     4201 accttcatcg ccacccacgg ccgcagcggc cacgacgagt ggttcttgtt gccgacgacg
     4261 tgcagcatcc cggccctcag cgcggcgcga gccaccccgg cgccacgccc tccagcgccg
     4321 caggctcgat gccgaaggga aagacgccgt cgcacacgct cggcacctgc atcgagtagt
     4381 agttgtcgcg cgtcatcagc ttcaccggcg cgagctccat caggcgcgcc tgccagtacg
     4441 acagccggtc gccgaggccg atgaccaggc gccgccgccc cgtgatgcgg cataccgcct
     4501 gcaccagctc gcgcagcgcg tactggcgcg ggccgcacag cgtgtagcgc cggcccaccg
     4561 cctcgcgcgc gtcgagcgcc gcgacgaacg cctgcgccac gtcggcgacg tacaccggct
     4621 ggaagcgcgc ttcggggcag gcgagcgcca gcaccggcag gaagcgcgcg agctgcgcaa
     4681 acaggttgag gaaaccgtct tccgggccga agatcaccga gggctggaac accgtcaccg
     4741 cgaggtcgtc ggcggcgagc gccgcctgct cgccgatgcc cttggagcgc agatactcgg
     4801 agggcgcctg cgggtcggcg ccgagcgcgc tcatgtgcag cagccgcttg gcgcctgccg
     4861 cccggcaggc acgcaccacg gcctgcggca gctccacgtg cgcgcgcgca aaatcgggac
     4921 cgtagtcgtt cgggccgcgc tcgtcgcgcc ggccgcgccg gctgtgcagc acgcccacca
     4981 ggttgacgac cgtgtccttg cccgcggcga gccgctcgag ctcgcccgcg cggccgacgt
     5041 cggcctcgac cacctcgacg gtgggcagca gcagcaggtg cttggcgcgt tcgcgccggc
     5101 gcgtgggcac cgtgacgcgc gcgccgcgtt tggcgagcgc cgcgaccaga tgccggccca
     5161 cgaagcccga gccgccgacg acgagaacgt cttcgtggcg catgctcagt ccgtgacctc
     5221 ggcggccgcg gcgcccgcgc tgcgcggcgt aatggtcccc aggcgcgcct tgagcggcgc
     5281 gctgcggccc tccagcagca gcgcgtaatg caccgtgttc gccatcacgc gcttcacgta
     5341 ctcgcgcgtc tcgtcgaacg ggatcgactc gatgaagatc gcaccttcga gcggccgcgc
     5401 cggttgccag cgcagcgcgc ggctcgggcc cgcgttatag gcggtcgagg cgagcaccgg
     5461 gtggccggtg cgctcgagca tgtgctgcag gtagcgcgcg ccgagcgtga cgttggtctc
     5521 gacctcggcg acgcgcttgg gcgaatagtc cttgtagccg atgcgccgcg ccacccagcg
     5581 cgcggtgtgc ggcaggatct gcatcaggcc ggtggcgccg gcacccgagc gcgcctcggg
     5641 gatgaagcgg ctctcctggc gcaccgtgcc gagcacccag gcctcctcca gcccgtaggc
     5701 gccggcgtat tcgcgaaaca cctcgcgata aggcgccagg tagcgcatgc ggtagtcgtg
     5761 cagcagctcc gtgcgcccgg cggtgccgat ggcgcgatcg aacaccgcgg cgcggcgcgc
     5821 gagctccgcc gccgcgagca gccgccggtc gcccatgtgg cgcaccgcaa acatccattc
     5881 gcgcgtggcc tcggcgcgca agcccagccg gtagagctcg agcgcgcgcg ccagttcggg
     5941 atgcgaccgc gccgcgtcca cctcggcttc gctcgcctcg tggtagggca tcgggatgct
     6001 cgcttcgccg ccgagctctt cggtcgcgag cacgctgtag tacagcggct gcccggcgat
     6061 ccgcaggtac tgctcgcgcg ccccctcggc atcgccgagc gcatcgcggg cgcggccgcg
     6121 ccagtaggtc caggcgagat cgagcccggc ctgcggcgac atcggttcga tcgcgtcgag
     6181 caccgcctgc caacgtcccg cgcgcagcgc cgcgcgcgcc ttccaggcga gctgcgtgtc
     6241 gtcgagcggt gcggcgccgg cgtgcgcgta ccagtcgagc gcctcgtcgc gatgctcgaa
     6301 cgcggcgagc gtggccaggc gcgcccacag atactcgcgc tcggccggcg gcaggcgtgc
     6361 gccggcgccc tcgcgcagcg cccgcgccgc ggcgtcgagg tcactgcgcg ccaggcgcag
     6421 cagcgcgaac accagcatct cgcgcgtcgc gcgcggctcg aggtcgcgcg gcccccgcgc
     6481 ggcgagctgc cgcggcgccg ttaccgcctg cgccagcagc cgctcgtcgg gccgcgccgc
     6541 tgccggcagg tcctccagcg tgcgccgcgc cgcggtgacg tagccgtagt acagctgcag
     6601 ccgcgcgcgc tgccagatgc gctcctcgtc cacggcattg cgcgccacca gctcgccgac
     6661 gagcttgcgg cagccctcgg gcagctcgcg cagctcggcg ctccagagcg cgcgcgcctc
     6721 gatttccgcc ggcacatcgc cgcgcgcgag gcgcgcgcgc cacgcgtggc agcggatctc
     6781 gagctcgtcc tgcaccagcg gccccagcgc gcgctcgaag gcgggccagt cgccgcgccg
     6841 gccgagctcc ttcagccagt cagcgcgcag cgtgtcgccg agataggtat tggcgtggcc
     6901 ggcaagatag cgctcgatgt cggcggcgtc cgcgtcctcg atgcgcagct tcaggcgcca
     6961 gtactcaacc cagggcgcga gcgtgtggtc ctcgagcgcg cgcgcgtggc gctcgaagcg
     7021 gtcggggtcg ccggcgcgaa aagcgtcgta agcgccgagc accgcgtcgt cgtttccttg
     7081 cgcgctgccc gccacagctg cggcgcaggc cagtgctata ttcaggaaac gcctataaat
     7141 catgaactaa gaatagcacg cgcgatgcga tcgcaagagc ttaaagcatg gcgcacggcc
     7201 gagcgcgaac gattgcttgc gttgcgcggc gcgctcgagg ccgacacgct cgaagc
//
LOCUS       DBNHKE010000224        27589 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_834975, whole genome shotgun sequence.
ACCESSION   DBNHKE010000224 DBNHKE010000000
VERSION     DBNHKE010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 27589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_834975"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..695
                     /locus_tag="AC16O9_15145"
     CDS             132..695
                     /locus_tag="AC16O9_15145"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6517990.1"
                     /translation="MHALKRLADAVDATNDWIGRAFAWLTLGMVLTEFTVVLSRYVFG
                     LGSTIMQESIVYMHATVFLVCAGYALAHNGHVRCDIFYATMTPRRRAIVDIIGTLVFL
                     LPMCVLIWWMGWPYVKASWAVMEVSQEGRLGIPAVYLLKTLILAFTGLLTFQALSLLL
                     QSALLVAGVEPNRGRVDGEPASGGPVV"
     gene            692..2257
                     /locus_tag="AC16O9_15150"
     CDS             692..2257
                     /locus_tag="AC16O9_15150"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6517991.1"
                     /translation="MKEVLALLMIVGLCGFVLIGYPVSFTLAGTALVFALAGMAFSVF
                     DPDFLGVLPHRIYGVMTNEVLIAIPLFIFMGVMLERSKVAEELLDTMGQLFGRLRGGL
                     GISVSVVGAMLAASTGIVGATVITMGLISLPTMLRRGYDPRLACGSIAAAGTLGQIIP
                     PSIVLVVLGDQISNAHQEAQRTLGNFSPEPVSVGDLFAGALIPGLALVGLYIFYQIII
                     AFVSPKSSPAIPRDESMQTGNMTARVMHALVAPLVLIVSVLGSILVGIATPTEAAGVG
                     AIGATLLAGHRLAEGKPIPVYTAAASLIGVIILTTFVDMRVTRTHITTAETISIGVAV
                     LLCLGIVWGLLVALWRVFRINTLQQVMRTTARLSTMIFAIIIAAQLFSLVFRGLGGDD
                     MVHKFLTGLPGGVIGAMIVVMGLLFVLGFFLDTIEILFVVVPIVGPVLLQFGLDPVWL
                     GIMIAVNLQTSFLTPPLGYALFYLRSVAPPEVTTMDIYRGVVPFVILQLMMLVILTYF
                     PAMATWLPKVIFG"
     gene            2338..3087
                     /locus_tag="AC16O9_15155"
     CDS             2338..3087
                     /locus_tag="AC16O9_15155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6517992.1"
                     /translation="MKVLVSVKRVVDYNVKVRVKADGTGVETANVKMSMNPFDEIAVE
                     EAVRLKEAGTVTEIVAVSCGPQACQETLRTALAIGADRAILVLAEGELQPLAVAKLLK
                     AVCEKEKPELVIMGKQAIDDDANQTGQMLAALLGRPQATFASKVKIADGKAEVTREVD
                     GGLDTLSIKLPAVITTDLRLNEPRYVTLPNIMKAKKKPLETLKPDELKVDIAPRLETL
                     KVVEPPKRKGGIKVSDAKALVDKLRNEAKVI"
     gene            3090..4028
                     /locus_tag="AC16O9_15160"
     CDS             3090..4028
                     /locus_tag="AC16O9_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6517993.1"
                     /translation="MTVLVFAEHDNQTVKKSTLNTVAAAQKIGGDIHLLVAGHECGAA
                     AQAAAQVAGVAKVLHADAKQLDDEIAENVAALMVGLAKNYSHLLAPATSNGKNVMPRV
                     AALLDVQQVSDIVSVESADTFVRPIYAGNALATVRSKDKIKVITVRTTGFDAVAASGG
                     SGAVEKVEAPADSGLSSFVGREVSKSERPELTSAKIIVSGGRGMANGENFKKVLEPLA
                     DKLGAAMGASRAAVDAGFVPNDWQVGQTGKIVAPDLYVAVGISGAIQHLAGMKDSRVI
                     VAINKDEEAPIFSVADYGIVGDLFQVVPQLLEELKK"
     gene            4041..5822
                     /locus_tag="AC16O9_15165"
     CDS             4041..5822
                     /locus_tag="AC16O9_15165"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6517994.1"
                     /translation="MSAYVAPLKDMKFVLQELAGLGAVGQLPGYQEASPDTVDAILEE
                     ASKFASQVLDPLNYSGDREGSKWSDGQVTTPKGFKDAYRQYVEAGWGALPISADWGGQ
                     GLPRLVATPVEEMWCSANMSFSLCPMLTQGAIHALELRGAPVLQKAFLPKMASGEWTG
                     TMNLTEPQAGSDLALLKTRAVPEGDHYRISGQKIFITFGEHDYTENIVHLVLARTPDA
                     PEGVKGISLFVVPKFLVNADGSLGARNGVACASIEHKLGIHGSPTAVMVFEGAIGYLV
                     GEENKGLAYMFIMMNAARFAVGLEGVAIAERAFQRALAYARERLQGRDLAGGGTVPII
                     RHPDVRRMLMLMKSQTEAMRALAYVVAAALDVAARHPDAKLREQNQAFVDLMIPVVKG
                     WCTETGIEVASLGVQVHGGMGFIEETGAAQHLRDARITTIYEGTTGIQANDLVGRKIA
                     REGGASAKAWIAHLQALDAELAKSGNADVKAIRAALATGAAAVSESIDYIVAQAGKDV
                     TAAFAGAVPFLKLMGVVAGGWQMARAALAAERLMASGDKAFLEAKLATARFYADQVLV
                     QAPGLSDTVVKGASAVMAVPEDAMLAV"
     gene            complement(5903..7414)
                     /locus_tag="AC16O9_15170"
     CDS             complement(5903..7414)
                     /locus_tag="AC16O9_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6517995.1"
                     /translation="MFARFWSSELPYVIVIALVLTGLLLRARPGERSSYQNTLWLFLA
                     GIIGQAASLGAAKLGFDAAARVVDVFFRIVWTIALIRIAGFAFFRLALPKLGKPLPRI
                     IEDLAIIVIYTVYGLSQLRHAGVDFTGILATSAIITAVLAFAMQDTLGNVLGGLAIQL
                     DNSVRVGDWIQLDGLAGRVIDIRWRSTAVETRDWQTVVIPNSQLMKNRFTILGRREGA
                     PLQWRRGIRFMIDPAVPPARVIALVDEEMREIMIANVARAPAPSCILIGFEAGNLLYE
                     LRYFLTDLQEDEFTDSAVRVHLFASLQRAGLRVAEEQRTVHAVAKDEAHAESVRQREL
                     QRRHIMLKQVDLLAPLSEEERNFVAERLQYAPFARGDIITKQGSIAHWLYVIAFGEAE
                     VRFEQPGRAPRVIGTVRPGQFFGEMGLLAGESRNATVVAKTDVECYRLDRASFQALLL
                     ARPELANEVSKVIAARKPELETARAEAAEAMANPPEPAQPALLERIQKFFGLK"
     gene            complement(7525..9153)
                     /locus_tag="AC16O9_15175"
     CDS             complement(7525..9153)
                     /locus_tag="AC16O9_15175"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR6517996.1"
                     /translation="MSRESMQYDVLVVGGGPAGLAAAIRLKQLAEELSVCVIEKGSEI
                     GAHILSGAVMDPRGLDELLPEAKSNGAPLGVPVSEDRFLFLTATGSFRTPNFLLPACF
                     RNHGNAVISLGNVTRWLGQQAEGLGVEIFPGFAGAEVLYDDKGAVRGVATGDMGVNRK
                     GEKTDAYQPGVELLAKYTFFAEGCRGHLGLQLEKKYGLRDGVDPQVYGIGLKELWEVK
                     PERHQPGLVVHTAGWPLERDTYGGSFLYHSENGLVAVGFVVGLSYTNPYLSPYEEFQR
                     YKTHPAIRGTFEGGKRIAYGARAIAAGGLQSLPKLVFPGGCLIGDDAGFLNASRIKGS
                     HAAIKSGMLAAEAAHEALKAGRAGDELAAYPEAFRKSWLFDELYRARNFKPWMSKGLV
                     TGSLMVGIDQVVFGGKAPWTLRHAHADHETLAQRTDVQPIDYPKPDGKLTFDRLTSVS
                     FSNTNHAEDQPVHLTLKDESVPVKVNLAQFDAPEQRYCPAGVYEIVRDGDEPRLQINA
                     QNCVHCKTCDIKDPTQNIVWVVPEGGGGPNYPNM"
     gene            9176..9601
                     /locus_tag="AC16O9_15180"
     CDS             9176..9601
                     /locus_tag="AC16O9_15180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6517997.1"
                     /translation="MSDIRRLAHVERIPIRWGDMDAMGHVNNTVYFRFMEQARISWFD
                     RLVPGEQAWQSTGVVIANASCNYRRAMTYPGTVEVRLFVGAPGGASVPTTYELRVDGD
                     PEPYADGAATVVFIDMKSQKSKRIPEEIRARLERAEAAA"
     gene            9598..11496
                     /locus_tag="AC16O9_15185"
     CDS             9598..11496
                     /locus_tag="AC16O9_15185"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR6517998.1"
                     /translation="MSGKPLWTPDPARAAQTGMARFMQLAGKKDYAELHRWSVEKRED
                     FWALLWQFAEVRGAPGKRRLVDGDRMPGAQWFPDARLNFAENLLRQRDDSLAIAFWGE
                     EQVKRRLTRAELHDLVSQLAQALRAAGVAKGDRVAAYLPNMPEATAAMLATASLGAIW
                     SSCSPDFGVQGVLDRFGQIEPKVLFCADRYFYGGKEFDLRAKNEQILAGLPSVRKAVS
                     APYAGGFGDFVAPFTPAALAFEQVEFNHPLYILYSSGTTGVPKCIVHGTGGTLLQHLK
                     EHLLHSDVRAGDRVYYFTTLGWMMWNWLMSGLGAGAALLLYDGSPFVSRGRIIFDMAD
                     AEGMTHLGTSAKFIDAIAKINLEPRVTHRLERVRAILSTGSPLVPEAFDYVYKNIKQD
                     VCLSSISGGTDIVSCFVLGNPMLPVWRGEIQCKGLGLAVEVFDDDGKPLSGEKGELVC
                     TKPLPAMPVGFWNDPDGAKYHAAYFDKYPNVWRHGDWCEITAHGGIVIYGRSDAVLNP
                     GGVRIGTAEIYRQVESLAEVVESLVIGQDGEGDVRVVLFVKLREGLKLDEALVAKIKK
                     HIRDNTTPRHVPAKVLQVGDIPRTKSGKIVELAVRHVVHGRPVKNVEALANPEAREHF
                     RDRAELAS"
     gene            11507..12865
                     /locus_tag="AC16O9_15190"
     CDS             11507..12865
                     /locus_tag="AC16O9_15190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6517999.1"
                     /translation="MSGGARALAIVLFGTVVAPLDSAVNAAFPAITAAFSLEVAAIQW
                     LVIAYMVTFSSLIIVSGRVADLFGHRRVFGWGLAVSALAFLGCGLAPGYGGLLAARVV
                     QGVGVAFLLGAGPALALGAYPATERTRVLARYAAAFAIGLAAGPALGGLLVDAFGWRA
                     VYLARVPIALGALLLLVYVPPTRIRPGRAFDSAGALLLAAWTGSLMLALTLRGAVGVG
                     LGAAAAAAFALFVAREARAAEPTIRVALFRDAAFAVPNLASVAVHFAGFGIFLLGPYF
                     FVRITGLSAQGAGLLLAVGPAAAALGSALAGRIAHAAGARHVALAGTLLNLAGVSITA
                     TWDAQTGVAAIAGTLALQGLGIGLFQVAYADTVIAALPAEDRGVAASLANLTRTFGVV
                     SCAALMSALFAAGERAALGGGAPPVAAFADGFAFAFGVAATGLTALLALSFVKGRAWR
                     DA"
     gene            12930..14597
                     /locus_tag="AC16O9_15195"
     CDS             12930..14597
                     /locus_tag="AC16O9_15195"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR6518000.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGIRVTNIKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFQSSKMRRANNFTTGQIYESVIKKE
                     RRGDYLGGTVQVIPHITDEIKQFIKKGAGDADVAIIEVGGTVGDIESLPFLEAIRQMG
                     LDLGRHNVCYVHLTLVPYIASAGEIKTKPTQHSVKELREIGIQADALLCRTDRPLPDD
                     ERRKIALFCNVQKEAVISAWDVDLIYKIPMMLHEQMLDEIVCHKLQILARAADLSPWV
                     KLVHALEHPEHEIDLAFVGKYVDLQDSYKSLNEALRHAGIHTRSRVNVHYFDSEEIEA
                     KGTAVLEPMDAILVPGGFGKRGTEGKMAAIRYARENGIPYLGICLGMQLATIEFARNV
                     CGLEGANSTEFDAETPHPVVALISEWLDRTGQVERRDAKSDLGGTMRLGAQKCPVEAG
                     TLAHRVYGAEVNERHRHRYEVNNVYVPQLEAKGYKVAARTPSERLPEIMELPAHPFFI
                     GVQFHPEFTSTPRKGHPLFKAFVEAAIEQRARAKRAPPGGSLSAVAA"
     gene            14594..15436
                     /gene="kdsA"
                     /locus_tag="AC16O9_15200"
     CDS             14594..15436
                     /gene="kdsA"
                     /locus_tag="AC16O9_15200"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR6518001.1"
                     /translation="MKLCGFEAGLDQPLFLIAGPCVVESRELQVDTAGRLKEICARLS
                     IPFIFKSSFDKANRSSSKSYRGLGMEQGLRILAEVKRQVGVPVLTDVHDEDEVKPAAE
                     VVDVLQTPAFLCRQTDFIRAVAAAGRPVNIKKGQFLSPQEMQNVVDKAREASGRDNVL
                     VCERGFSFGYGNLVSDMRSLAIMRATGCPVVFDATHSVQLPGGQGTSSGGQREFIPVL
                     ARAAVASGISGLFMETHPNPEIALSDGPNAWPLGLMPPLLETLAALDRLVKESGFAEN
                     QVKH"
     gene            15455..16738
                     /gene="eno"
                     /locus_tag="AC16O9_15205"
     CDS             15455..16738
                     /gene="eno"
                     /locus_tag="AC16O9_15205"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6518002.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLLESGSMGRAAVPSGASTG
                     RREAVELRDGDARRYGGKGVLKAVEHINTEISEAIVGLDASEQGFIDKTLVDLDGTEN
                     KSRLGANALLAVSMAVAKSAAEESGLPLYRYFGGSGPMQMPVPMMNVVNGGAHANNNL
                     DIQEFMIVPVGAKSFSDALRCGAEVFQALRKLVGERGSSTAVGDEGGFAPDLPNHAAA
                     IELILEAIGKAGYKPGSDVALALDCAATEFHKNGRYVLASEKLELNAQEMCDYLAAFA
                     ERYPIVSIEDGLAEDDWDGWKRLTERLGSKLQLVGDDLFVTNVRTLREGVKRGVANAI
                     LVKVNQIGTLTETFAAIELAKRSGYAAVISHRSGETEDTTIADIAVGTNALQIKTGSL
                     SRSDRVAKYNQLLRIEEDLGDSVSYPGREAFARLR"
     gene            16740..17021
                     /gene="ftsB"
                     /locus_tag="AC16O9_15210"
     CDS             16740..17021
                     /gene="ftsB"
                     /locus_tag="AC16O9_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HIR6518003.1"
                     /translation="MKILGGVLAALILLIQYPLWLGKGGWLRVSEVDRQLSAQQAKNG
                     ALEARNGALEAEVRDLKQGLEAIEERARYELGMIKSDEVFFQVVEKPGK"
     gene            17166..18524
                     /locus_tag="AC16O9_15215"
     CDS             17166..18524
                     /locus_tag="AC16O9_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR6518004.1"
                     /translation="MNLHAKLKEREAQGKPLRLGVIGAGKFATMYIAQVPRTPGVHLV
                     GIADLNPAYAKANLERTGWAPEHFGASSLDAAAKERRTHLGEDWRALVAHPAIDIVIE
                     ATGNPLAAVEHALEAFRNGKHVVMVTVEADAFCGPLLAQKAAAAGVVYSLAYGDQPAL
                     ICDLVDWARAAGFPVVAAGRGHKWQAHFAQSTPETVWGYYGLTPEQAKVGGLNPKMFN
                     SFLDGSKPAIETSAVCNATGLTPAPGGLAYPAGRIDQIPNLMRPRAEGGVLHHKGQVE
                     VVSSLQPDGTPVDYDIRFGVFVSFEGESDYIRRCFAEYGVKTDDSGRYACLYKRWHLI
                     GLEVGISVASVGLRGEPTGCATGFRADAVATAKRTLKAGEVLDGEGGYTVVGRLMPAA
                     ESLAQGCLPLGLAHGWKLLKPVAAGQPLRWSDVAFDAEVAAVKLRREMEAACRAAERH
                     AA"
     gene            18514..18765
                     /locus_tag="AC16O9_15220"
     CDS             18514..18765
                     /locus_tag="AC16O9_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518005.1"
                     /translation="MPPEGAPRFLTKFAFRIRTRSGTPVENLMLQAADRAQAEARIQQ
                     MYPYCEIVECRELTATPRDETASLDSILSIISREEGEPR"
     gene            complement(18762..19550)
                     /locus_tag="AC16O9_15225"
     CDS             complement(18762..19550)
                     /locus_tag="AC16O9_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6518006.1"
                     /translation="MAKIAVFNQKGGVGKTTTSLNLTAALARRGFNPLSIDLDPQAHL
                     SYVCGAAVERSDDSIFGFYQQAKPLTQLIRPATGGWLAISAHVELSKVDTHYGKGANV
                     LNRLNLGIIRENLNTGRPIVIDCCPVLGVLSLSAIFAADRVLIPVSADYLALKGVQQC
                     EKTLKALEHVLKKRVARRYVVTRFDTRRKLSHQIVGELHARFGMDICGTRISENVALA
                     ESPASCKDVFAHAPESRGAQDYDALVEELIATGFIERRPPPPAA"
     gene            complement(19601..19993)
                     /locus_tag="AC16O9_15230"
     CDS             complement(19601..19993)
                     /locus_tag="AC16O9_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6518007.1"
                     /translation="MDKRSQKERFDDGLKTRREVLGAAYVDKAMAGVDDFSKPFVDLL
                     NTYCWNDIWNRPGLDRKTRSMLNLAMLSALGKEHELKLHINGALNNGLSKEQIREVFL
                     QVAIYCGVPSAVVAFRCAKEVFKDRGTS"
     gene            complement(20007..20780)
                     /locus_tag="AC16O9_15235"
     CDS             complement(20007..20780)
                     /locus_tag="AC16O9_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR6518008.1"
                     /translation="MDLSDKLLRLKPAAIDDDEWVVRLELAALYRAFDWLGWSESIFN
                     HITARVPGGERHYLINPYGLNYSEVTATNLVKINLKGEVMDGSKYPVNVAGFVIHSAI
                     HGAREDAHCIIHTHSTAGSAVASKKDGLRFDNFYSALLYGQVAYHDFEGVTTDLSEQA
                     RLVKSIGDKAILILRNHGLLVACPGIPQAYLTYWGLQRACEIQVATDAMRGENLPISQ
                     RVLDETPARSQAFNKDPRPGGPPFDALLRRAGIRYEEII"
     gene            complement(20801..21946)
                     /locus_tag="AC16O9_15240"
     CDS             complement(20801..21946)
                     /locus_tag="AC16O9_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518009.1"
                     /translation="MTLGGAGMYIVTVALPLVQAEYGVDRSDASLPYTLTMIGFGLGG
                     IVMGRLADRFGVLVPVVIGTVCLGAGFVVAGFAGSLWQFALAQGLLIGFLGCSATFAP
                     LVADTSLWFHRRRGIAVGICASGNYLAGALWPPVVQHYFDTVGWRQTYMGVGVFCIAA
                     MLPLALVLRRRPPVLVVTSADGTGHAPAPQSLGLRPGLVLGLLCTAGVACCVAMSMPQ
                     VHIVALCGDLGFAAARGAEMLSLMLGFGIVSRLLSGWICDRIGGLRTLLLGSTLQMLA
                     LGLYLGADDLTSLYVVSAMFGLFQGGIVPSYAIIVREYFAPRVAGTFVSIVLTSTLFG
                     MALGGWMSGAIFDLTGSYRAAFLNGIGWNLLNMAIAGTLLLRARKRL"
     gene            complement(22013..22660)
                     /locus_tag="AC16O9_15245"
     CDS             complement(22013..22660)
                     /locus_tag="AC16O9_15245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6518010.1"
                     /translation="MRSSVAAFVLLLAAGCATAQSPHTHQHSFRDAEKWSHIFDDPQR
                     DAWQKPHEVIQALAIKPGALVADLGAGTGYFSARLANMLPGGRIYAVDIEPDMVRYLE
                     ARAKREGLRNIVPVKGEPDDPRLPAQVDLILLVDVYHHIGERSAYFSRLRGTLRSGGR
                     IAIVDFKLDSPVGPPPAGRIAPETVKAEMKAAGYRVAGEHSFLPYQYFLVFAPAS"
     gene            22662..23471
                     /locus_tag="AC16O9_15250"
     CDS             22662..23471
                     /locus_tag="AC16O9_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6518011.1"
                     /translation="MASLSGLRSGTTLVLLHGLASNATRWWHFAAHAQLPGLKLLRPT
                     LRGGAGSSDRGRIDMRVWCDDLVRLLDAENCERAVVGGHCLGANIALHFASRHPGRTA
                     GLVLIEPMPPQALAGSMRRIRFFRRPILALAAVVRALNALGIRRAGFEPLDLEQWDRA
                     LARDEGDLGRYASPLSDLRLAPVAAYLQSLAALGEPLPPLASIRAPALVLLSRSSTMT
                     DPVRTRAILEELPRVEFKVLEAEHWIPTEQPEDMRMAIEAWLAANAASLAQ"
     gene            complement(23475..23550)
                     /locus_tag="AC16O9_15255"
     tRNA            complement(23475..23550)
                     /locus_tag="AC16O9_15255"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23515..23517),aa:Asn,seq:gtt)
     gene            complement(23569..24783)
                     /locus_tag="AC16O9_15260"
     CDS             complement(23569..24783)
                     /locus_tag="AC16O9_15260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HIR6518012.1"
                     /translation="MNAPHAPSLLASVPMAPRDPILGVTELYVADKNPRKVNLGVGVY
                     YDDKGKVPLLECVQRAEQQRVQTAAPKSYLPIDGLQAYDQAVQALVFGADAPVLKEKR
                     VVTVQAIGGTGGLKIGADFLKLLAPAAQVWISEPSWENHRALFEAAGFKVQAYPYYDA
                     ATRGLKFEAMCQALEALPAGTLVVLHSCCHNPTGVDLSQEQWRKVLAIVQARALLPFL
                     DLAYQGFAEGIDADAYAPRLFAAAMSPIFLSSSFSKSLSLYGERVGAFTLVAGSADEA
                     ARALSHVKRVVRTNYSNPPTHGGDIVARVLNTPELRALWEKELAAMRDRIKAMRKALA
                     DGVQKRSGKDFSFVLKQRGLFSYTGLTKDQVERLRKEHSIYAIDTGRVCVAALNSGNI
                     DTVAEAIAGVIR"
     gene            24825..26855
                     /gene="uvrB"
                     /locus_tag="AC16O9_15265"
     CDS             24825..26855
                     /gene="uvrB"
                     /locus_tag="AC16O9_15265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR6518013.1"
                     /translation="MALAFPNSPFRLHQPFAPAGDQPEAIEALTEGVRDGLAFQTLLG
                     VTGSGKTYTMAHVIARLGRPALVLAPNKTLAAQLYSEFREFFPENAVEYFVSYYDYYQ
                     PEAYVPARDLYIEKDSSINEHIEQMRLSATKSLMERRDAVIVATVSAIYGIGDPSEYH
                     GMILHLRQGEKATQREVIARLVAMQYERNELDFRRGTFRVRGDVIDVFPAEHAEHALR
                     LSLAEDEIERLQLFDPLTGRVLHGLTRFTVYPSSHYVTPRETTLRAIEAIKAELRERI
                     EFFQKAGRLVEAQRIEQRTRFDLEMLVELGFCKGIENYSRHLSGRKAGEPPPTLIDYL
                     PPDALMIIDESHVTIGQLGGMYKGDRSRKENLVEYGFRLPSALDNRPLRFDEFEGVVR
                     QTVFVSATPADYEAQKSAQVVEQVVRPTGLVDPAVEVRPASSQVDDLLSEIRLRSAKR
                     ERVLVTTLTKRMAEELTEYLSEHGVKVRYLHSEVDTVERVEIIRDLRLGEFDVLVGIN
                     LLREGLDLPEVSLVAILDADKEGFLRSERSLIQTMGRAARHLNGRAILYADVVTESMQ
                     RALGETERRRRKQLTWNASHGITPRGVSKRIKDLIDGVYDAEDAAAELRAAQDEARYE
                     AMSEKQVAREIRRLEKDMIGHARNLEFEKAAQLRDQLAALKKRVFGVELAPE"
     gene            complement(26865..>27589)
                     /locus_tag="AC16O9_15270"
     CDS             complement(26865..>27589)
                     /locus_tag="AC16O9_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518014.1"
                     /translation="GERRPDQRPQHSQQRPPHQQQRPPQPQPGQQPQGPRPAESALTG
                     TRDGEEHRRGRRGRGRDRNRDRRPDGSRDGQGQGGRAPEQAGSLPADPGIPSYLAREP
                     DEKELRAREREMHEAQMREEARLNAEAEQRAAQVQPEPQPEREMREPRPQPAPEREMR
                     EPRPQPAPEREIPASPPAMAKVDPREVLESAGLQMVETRPDRVPAALAEPEAVPLGRP
                     RRERSRPAAAEDEPLVQVETKN"
BASE COUNT         4328 a         9283 c         9623 g         4355 t
ORIGIN      
        1 atcggctgga gcgcgctcgg cgagcagggc tacatgaacg cccgttcgct gatcaagttc
       61 tagacgtctg tcgcgaggta actacagcct aggcgcgggc aacccccgcg cctaggttct
      121 tctgcacccg catgcacgca ctcaagcgtc tcgccgacgc ggtcgacgcg accaacgact
      181 ggatcgggcg cgcctttgcc tggctcacgc tcggcatggt gctcaccgag ttcaccgtgg
      241 tgctgtcgcg atatgtgttc gggctgggct cgaccatcat gcaggagtcg atcgtctaca
      301 tgcacgccac cgtgttcctg gtgtgcgcgg gctatgcgct ggcgcacaac ggccacgtgc
      361 gctgcgatat tttctacgcc acgatgacgc cgcgccgccg ggcgatcgtg gacatcatcg
      421 gcacgctggt gttcctgctg ccgatgtgcg tattgatctg gtggatgggc tggccttacg
      481 tcaaggcgtc gtgggccgtg atggaggtct cgcaggaggg gcggctgggt atcccggcgg
      541 tgtatctcct gaagacgctg atcctggcgt tcacggggct cctcaccttc caggcgctgt
      601 cgctgctgct gcagtccgcg ctgctggtgg ctggcgtcga gccgaaccgg ggccgcgttg
      661 acggcgagcc ggcgagcggg gggccggtcg tatgaaggaa gttctcgcgc tgctgatgat
      721 cgtcggcctg tgcggcttcg tgctgatcgg ctacccggtg tccttcacgc ttgccggcac
      781 tgcgctcgtc ttcgcgctgg ccggcatggc gttcagcgtg ttcgacccgg acttcctcgg
      841 cgtgctgccg caccgcatct acggggtgat gaccaacgag gtgctgatcg cgattccgct
      901 gttcatcttc atgggcgtga tgctcgagcg ctcgaaagtc gccgaagagc tgctcgacac
      961 catgggacag ctgttcggcc ggctgcgcgg cgggttgggc atttccgtct cggtggtcgg
     1021 cgcgatgctt gccgcctcca ccggcatcgt cggcgccacc gtgatcacca tgggactcat
     1081 ttccctgccg acaatgctgc gccgcggcta cgatcccagg ctcgcctgcg gctcgatcgc
     1141 cgccgccggc acgctcggac agatcattcc gccgtccatc gtgctggtgg tgctcggcga
     1201 ccagatctcc aacgcacacc aggaggcgca gcgcacgctg ggcaacttct cgcccgagcc
     1261 ggtctcggtg ggcgacctgt tcgccggcgc gctgatcccg gggcttgcgc tcgtcggcct
     1321 ctacatcttc taccagatca tcatcgcgtt cgtcagcccg aagtcctcgc cggcgatccc
     1381 gcgcgacgag tcgatgcaga cgggcaacat gacggcccgt gtcatgcacg cgctagtcgc
     1441 gcccctggtg ctgatcgtgt cggtgctcgg ctcgatcctg gttggcatcg ccaccccgac
     1501 cgaggcggcg ggcgtgggcg ccatcggcgc cacgctgctc gccgggcacc gcctcgcaga
     1561 aggcaagccg atccccgtct acactgccgc cgcctccctg atcggggtca tcatcctcac
     1621 gaccttcgtc gacatgcggg tgacgcgcac gcacatcacc acggctgaaa cgatctcgat
     1681 cggcgtcgcc gtgctgctgt gcctgggcat cgtctgggga ttgctggtcg cgctctggcg
     1741 cgtgttccgc atcaacacgc tgcagcaggt gatgcgtacc accgcgcgcc tgagcacgat
     1801 gatcttcgcc atcatcattg ccgcgcagct tttttcgctc gtgttccgcg ggctgggcgg
     1861 cgacgacatg gtgcacaagt tcctcaccgg gctgcccggc ggggtgatcg gcgcgatgat
     1921 cgtggtgatg gggctgctgt tcgtgctggg cttcttcctc gacaccatcg agatcctgtt
     1981 cgtcgtcgtg ccgatcgtcg ggccggtgct gctgcagttc ggcctcgacc cggtgtggct
     2041 cggcatcatg atcgcggtca acctgcagac ttccttcctc acgccgccgc tcggctacgc
     2101 gttgttctac ctgcgcagcg tcgcgccgcc cgaggtcacc acgatggaca tttaccgcgg
     2161 cgtggtgccc ttcgtgatcc tccagctcat gatgctggtg atcctgacct acttcccggc
     2221 gatggcgacc tggctgccga aagtcatctt cggctgacgc tgctggtcgt ttcaaacgct
     2281 cggcaggcgc ggggcgccgg gctataattt gccgctcttt cacctcatga ccgcctcatg
     2341 aaagtcctgg tcagcgtcaa gcgcgtggtc gattacaacg tcaaggtgcg cgtcaaggcc
     2401 gacggcacgg gcgtagagac cgccaacgtc aagatgtcga tgaacccgtt cgacgaaatc
     2461 gcggtcgagg aggcggtgcg gctcaaggaa gccggcacgg tgaccgagat cgtcgccgtg
     2521 tcctgcggcc cgcaggcgtg ccaggagacg ctgcgcacgg cgctcgcgat cggcgccgac
     2581 cgcgccatcc tggtgctggc cgaaggcgag ctgcagccgc tcgcggtggc aaagctgctc
     2641 aaggcggtgt gcgagaagga aaagcccgag ctcgtgatca tgggcaagca ggcgatcgac
     2701 gacgacgcga atcagacggg ccagatgctc gccgcgctgc tcggccggcc gcaggccacc
     2761 ttcgcctcga aagtgaagat cgcggacggc aaggccgagg tgacgcgcga ggtggacggc
     2821 ggcctcgaca ccctctccat caagctgccg gcggtgatca ccaccgacct gcgcctgaac
     2881 gagccgcgct acgtcacgct gccgaacatc atgaaggcga agaaaaagcc cctcgagacg
     2941 ctcaagcccg acgagctcaa ggttgacatc gccccgcggc tggagacgct caaggtcgtc
     3001 gagccgccga agcgaaaggg cggcatcaag gtttccgacg ccaaggcgct ggtcgacaag
     3061 ctgcgcaacg aagcgaaggt gatatagcga tgacggtcct cgtctttgcg gagcacgaca
     3121 accagaccgt caagaagtcg acgctcaaca cggtcgcggc cgcgcagaag atcggcggcg
     3181 acatccatct gctcgtggcc ggacacgaat gcggcgcggc ggcgcaagct gccgcgcagg
     3241 tcgcgggcgt ggccaaggtg ctgcacgccg acgccaagca gctcgacgac gagattgccg
     3301 agaacgtcgc cgcgctgatg gtcggcctcg ccaagaacta ctcgcacctg ctcgcgccgg
     3361 cgacgtcgaa cggcaagaac gtgatgccgc gcgtcgcggc gctgctcgac gtgcagcagg
     3421 tctcggacat cgtgtcggtc gagagcgcgg acacgttcgt gcggccgatc tatgccggca
     3481 acgcgcttgc cacggtcagg tcgaaggaca agatcaaggt gatcacggta cgcaccaccg
     3541 ggttcgacgc ggtggccgcg agcggcggca gcggggcggt ggaaaaggtc gaggcgcccg
     3601 ccgacagcgg cctgtcgtcg ttcgtcggtc gcgaggtgtc caagtccgag cgcccggagc
     3661 ttacttcggc caagatcatc gtgtcgggcg gtcgcggcat ggccaacggc gagaacttca
     3721 agaaggtgct cgagccgctc gccgacaagc tcggcgcggc gatgggcgcc tcgcgcgccg
     3781 cggtcgacgc gggtttcgtg ccgaacgact ggcaggtggg gcagaccggc aagatcgtcg
     3841 cgccggacct gtacgtcgcg gtcggcatct cgggcgccat ccagcacctc gccggcatga
     3901 aggacagccg cgtcatcgtc gccatcaaca aggacgagga ggcgccgatc ttctcggtgg
     3961 ccgactacgg catcgtcggg gacctgttcc aggtcgtgcc gcagctgctc gaggaactga
     4021 agaagtaaac ggaggtcgcc atgtccgcgt acgtcgcgcc gctgaaggac atgaagttcg
     4081 tgctgcagga gctggccggc ctcggcgcag tagggcagct ccctgggtac caggaggcca
     4141 gcccggatac ggtcgacgca atacttgagg aggcttcgaa gtttgcgtca caagtgcttg
     4201 atccattgaa ctatagtggt gatcgtgagg gctccaaatg gagcgatggg caggtcacca
     4261 cgcccaaggg cttcaaggac gcctaccgcc agtacgtgga agccgggtgg ggtgcgctgc
     4321 cgatcagtgc cgattggggc gggcagggcc tgcccaggct ggtggccacg ccggtcgaag
     4381 agatgtggtg cagcgcgaac atgagcttct cgctgtgccc gatgctcacc cagggtgcga
     4441 tccacgcgct cgagctgcgc ggcgcgcccg tgctgcagaa ggccttcctg ccgaagatgg
     4501 cgagcggcga gtggaccggc accatgaatc tcaccgagcc gcaggcgggc tccgatctgg
     4561 cgctgctcaa gacgcgtgcg gtgcccgaag gggaccacta ccgcatctcc gggcagaaga
     4621 tcttcattac gttcggcgag cacgactaca ccgagaacat cgtgcacctg gtgctcgcgc
     4681 gcacgcccga tgcgcccgag ggcgtgaagg gcatttcgct cttcgtggtg cccaagttcc
     4741 tggtcaatgc cgacggctcg ctcggcgcgc gcaacggcgt ggcctgcgcc tcgatcgagc
     4801 acaagctcgg catccacggc agcccgaccg cagtgatggt cttcgagggc gcgatcggct
     4861 acctggtcgg cgaggagaac aaggggctcg cctacatgtt catcatgatg aacgccgcgc
     4921 gcttcgcggt cggcctggag ggcgtggcca tcgccgagcg cgccttccag cgcgcgctcg
     4981 cctatgccag ggagcgcctg caggggcgcg atctcgccgg cgggggcacg gtgccgatca
     5041 tccggcatcc cgacgtgcgc cgcatgctca tgctgatgaa gtcgcagacc gaggcgatgc
     5101 gcgcgctcgc ctacgtggtg gcggcggcgc tcgatgtcgc ggccaggcac ccggacgcga
     5161 agctgcgcga gcagaaccag gcgttcgtcg acctcatgat cccggtggtg aagggctggt
     5221 gcaccgaaac cggcatcgag gtcgcctcgc tcggcgtgca ggtgcacggc ggcatgggct
     5281 tcatcgagga aaccggcgcc gcgcagcatc tgcgcgacgc gaggatcacc accatctacg
     5341 aaggcaccac cggcatccag gcgaacgacc tcgtcggccg caagatcgcg cgcgagggcg
     5401 gggcgagcgc caaggcctgg atcgcgcacc tgcaggcgct cgatgcggag ctcgcgaaat
     5461 cgggcaacgc cgacgtgaag gcgattcgcg cggcgctcgc cacgggcgcc gcggcggtga
     5521 gcgagagcat cgactacatc gtcgcccaag cgggcaagga cgtgacggcg gcgttcgccg
     5581 gcgcggtgcc gttcctgaag ctcatgggcg tggttgccgg cggctggcag atggcgcgcg
     5641 ccgcgctcgc cgcggagcgg ctgatggcgt ccggcgacaa ggcgttcctc gaggccaagc
     5701 tcgccaccgc gcgcttctac gccgaccagg tcctggtgca ggcgccgggg ctgagcgata
     5761 cggtagtgaa gggcgccagc gccgtcatgg cggtgcccga ggacgcgatg ctggccgttt
     5821 gactcgttct tcttgccgct tctaaatggg gacagacctc atttttcttt tcacgatatg
     5881 aaaagaaaaa tgaggtctgt ccctatttaa gcccgaagaa cttctgaatt ctttccagta
     5941 gcgccggctg cgccggctcg ggcgggtttg ccatcgcctc ggccgcttcg gcgcgcgcgg
     6001 tttcgagctc gggcttgcgc gcggcgatca ccttgctgac ctcgttggcg agctcgggcc
     6061 gcgcgagcag cagcgcctgg aacgaggcgc ggtccaggcg gtagcactcg acgtcggtct
     6121 tggccaccac ggtggcgttg cgcgattcgc ccgcgagaag ccccatctcg ccgaagaact
     6181 gtcccgggcg cacggtgccg atgacgcgcg gggcgcggcc cggctgctcg aagcgcacct
     6241 cggcctcgcc gaaggcgatg acgtagagcc agtgcgcgat cgagccctgc ttggtgatga
     6301 tgtcgccgcg cgcaaatggc gcgtactgca ggcgctcggc gacgaagttg cgctcctctt
     6361 cggagagcgg cgcgagcaga tccacctgct tgagcatgat gtggcggcgc tgcagctcgc
     6421 gctggcgcac gctttcggca tgcgcctcgt ccttggccac cgcgtgcacc gtgcgctgct
     6481 cctcggccac gcgcaagccc gcacgctgga gcgaggcaaa caggtgcacg cgcaccgccg
     6541 agtcggtgaa ctcgtcctcc tgcaggtcgg tgaggaagta gcgcagctcg tagagcaggt
     6601 tgcccgcctc gaagccgatc agaatgcagc tcggcgccgg cgcgcgcgcc acgttggcga
     6661 tcatgatctc gcgcatctcc tcgtcgacga gcgcgatgac gcgcgccggc ggcacggccg
     6721 ggtcgatcat gaagcggatg ccgcggcgcc attgcagcgg cgcaccctcg cgccgcccga
     6781 ggatggtgaa gcggttcttc atcagctggc tgttggggat caccacggtc tgccagtcgc
     6841 gggtctccac cgccgtggag cgccagcgga tgtcgatcac ccggccggcg aggccgtcca
     6901 gctggatcca gtcgccgacg cgcaccgagt tgtcgagctg gatggcgagg ccgccgagca
     6961 cgttgccgag cgtgtcctgc atggcgaaag cgagaaccgc ggtgatgatc gcggacgtgg
     7021 cgaggatgcc ggtgaagtcg accccggcgt gccgcagctg cgacaggccg tacaccgtat
     7081 agatgacgat gatcgccagg tcctcgatga tgcgcggcag cggcttgccg agcttgggca
     7141 gcgcgaggcg gaagaatgcg aagccggcga tgcgtatcag cgcgatcgtc cagacgatgc
     7201 ggaagaagac gtcgacgacc cgcgccgcag cgtcgaagcc gagcttggcc gcgccgaggc
     7261 ttgcagcctg gccgatgatg ccggcgagga acagccacag cgtgttctgg tagctcgagc
     7321 gctcgcccgg acgcgcgcgc agcagcagcc ccgtcagcac cagcgcgatg acgatgacgt
     7381 acgggagctc ggagctccag aagcgggcga acacggtcag gggcctagaa cttgatcttg
     7441 ccggcgagga tgtccttcga catcccccag tctcccacga agccgggcaa ggggtagaac
     7501 tcggcgaagt tcttcatgcc agcctcacat gttcgggtag ttcggcccgc cgccgccctc
     7561 gggcaccacc cagacgatgt tctgcgtcgg gtccttgatg tcgcaggtct tgcagtgcac
     7621 gcagttctgc gcgttgatct gcagccgcgg ctcgtcgccg tcgcgcacga tctcgtagac
     7681 gcccgcgggg cagtagcgct gctcgggcgc gtcgaactgc gccaggttca ccttcaccgg
     7741 cacgctttcg tccttcagcg tgaggtgcac cggctggtcc tcggcgtggt tggtgttgga
     7801 gaacgagacc gaggtcagcc ggtcgaaggt gagcttgccg tcgggcttgg ggtagtcgat
     7861 gggctgaacg tcggtgcgct gcgccagcgt ctcgtggtcg gcgtgcgcgt ggcgcagcgt
     7921 ccacggcgcc ttgccgccga agaccacctg gtcgatgccg accatcagcg agccggtgac
     7981 cagccccttc gacatccacg gcttgaagtt gcgcgcgcgg tacagctcgt cgaacagcca
     8041 ggacttccgg aacgcctccg ggtacgccgc gagctcgtcg cccgcgcggc ccgccttgag
     8101 cgcctcgtgc gccgcctcgg cggcgagcat gcccgacttg atcgccgcgt ggctgccctt
     8161 gatgcgcgag gcgttgagga agccggcgtc gtcgccgatc aggcagccac cggggaagac
     8221 cagcttcggc agcgactgca gcccgccggc agcaatcgcc cgcgcgccgt aggcgatgcg
     8281 cttgccgccc tcgaacgtgc cgcggatggc ggggtgcgtc ttgtagcgct ggaactcttc
     8341 gtacggcgag aggtacgggt tggtgtagct cagcccgacg acgaagccga cggcgacgag
     8401 gccgttctcg gagtggtaca ggaacgagcc gccgtaggtg tcgcgctcga gcggccagcc
     8461 ggccgtgtgc acgacgagcc ccggctggtg ccgctcgggc ttcacttccc acagctcctt
     8521 cagcccgatg ccgtagacct ggggatccac gccgtcgcgc agcccgtact tcttctccag
     8581 ttgcaggccg aggtggccgc ggcagccttc ggcgaagaac gtgtacttcg ccagcagctc
     8641 cacgcccggc tggtaggcgt cggtcttttc gcccttgcgg ttcacgccca tgtcgcccgt
     8701 ggcgacgccg cgcactgcgc ccttgtcgtc gtagagcact tcggcgccgg cgaagccggg
     8761 gaagatctcc acgccaaggc cttcggcctg ctggccgagc cagcgcgtga cgttgcccag
     8821 gctgatgacg gcgttgccgt ggttcctgaa gcacgcgggc aggaggaagt tcggcgtgcg
     8881 gaacgatccg gtggcagtga ggaacaggaa gcggtcctcg ctcaccggca cgccgagcgg
     8941 cgcgccgttg gatttcgcct ccggcagcag ctcgtcgagc ccgcgcggat ccatcacggc
     9001 gccggagagg atgtgcgcgc cgatctcgga gcctttctcg atcacgcaga cggaaagttc
     9061 ttccgcgagc tgcttgagcc ggatcgcggc ggcgaggccg gcagggccgc cgcccaccac
     9121 cagcacgtcg tactgcatcg attcgcggct catgcgggca ttatattatc cgggcatgag
     9181 cgacatccgc cggctcgcgc acgtcgagcg catcccgatc cgctggggcg acatggacgc
     9241 gatgggccac gtcaacaaca ccgtgtactt ccgcttcatg gagcaggcgc gcatcagctg
     9301 gttcgaccgc ctggtgcccg gcgagcaggc ctggcaatcc acgggcgtgg tgatcgccaa
     9361 cgcctcgtgc aattaccggc gcgccatgac ctacccgggc accgtggagg tgcggctctt
     9421 cgtgggcgca cccggcggcg ccagcgtgcc gaccacctat gagctgcgcg tggatggcga
     9481 cccggagccg tacgccgacg gcgccgccac ggtggtgttc atcgacatga agtcgcagaa
     9541 atccaagcgc atcccggaag agatccgcgc gcgcctggag cgcgcggaag cggccgcatg
     9601 agcggcaagc cgctgtggac gcccgacccc gcgcgcgccg cgcagactgg catggcgcgc
     9661 ttcatgcagc tcgcgggcaa gaaggactac gccgagctgc accgctggtc ggtggaaaag
     9721 cgcgaggact tctgggcgct gctctggcag ttcgccgaag tgcgcggcgc gccgggcaag
     9781 cggcgcctgg tggacggcga tcgcatgccc ggcgcgcagt ggtttcccga tgcgcgtctc
     9841 aacttcgccg agaacctgct gcgccaacgc gacgattcgc tcgccatcgc gttctggggc
     9901 gaggagcagg tcaagcggcg tctgacgcga gccgagctgc acgatctcgt gtcgcagctg
     9961 gcgcaggcgc tgcgcgcggc cggggtggcg aagggcgacc gcgtggcggc ctacctgccc
    10021 aacatgcccg aggccaccgc ggcgatgctg gccacggcga gcctcggcgc catctggtcg
    10081 agctgctcgc ccgatttcgg cgtgcaaggc gtgctcgacc gcttcggcca gatcgagccc
    10141 aaggtgctct tttgcgccga ccgctacttc tacggcggca aggaattcga cctgcgggcg
    10201 aagaacgagc agatcctcgc cgggctgccg agcgtgcgca aggcggtcag cgcgccctac
    10261 gcgggcggct tcggcgattt cgtcgcaccg ttcacgcccg ccgcgctcgc gttcgagcag
    10321 gtggaattca accacccgct ctacatcctg tactcctcgg gcaccacggg cgtgcccaag
    10381 tgcatcgtgc acggcaccgg cggcacgctg ctgcagcacc tgaaggagca cctgctgcac
    10441 agcgacgtgc gggcgggcga ccgcgtgtac tacttcacca cgctgggctg gatgatgtgg
    10501 aactggctga tgtccggcct cggggccggc gccgcgctgc tgctctacga cggctcgccc
    10561 ttcgtcagcc gcggccgcat catcttcgac atggcggacg ccgagggcat gacgcacctg
    10621 ggcacctcgg ccaaattcat cgatgccatc gccaagatca acctggagcc cagggtgacg
    10681 caccgcctgg agcgcgtgcg cgccatcctc tcgacgggct cgccgctggt gcccgaggcc
    10741 ttcgactacg tctacaaaaa catcaagcaa gacgtctgcc tcagctcgat ctcgggcggc
    10801 accgacatcg tcagctgctt cgtgctcggc aacccgatgc tgccggtgtg gcgcggcgag
    10861 atccagtgca agggcctggg gctcgcggtg gaggtgttcg acgacgacgg caagccgctg
    10921 tccggggaga agggcgagct ggtgtgcacg aagccccttc cggcgatgcc ggtcggcttc
    10981 tggaacgatc ccgacggcgc gaagtaccac gccgcctact tcgacaagta ccccaacgtc
    11041 tggcgccacg gcgactggtg cgagatcacc gcgcacggcg gcatcgtcat ctacggccgc
    11101 tccgatgccg tgctcaatcc gggcggggtg aggatcggca ccgccgagat ctaccgccag
    11161 gtggagagcc tggcggaggt ggtggagtcg ctggtcatcg gccaggacgg ggagggtgac
    11221 gtgcgcgtgg ttctgttcgt caagctgcgc gaaggcctca agctcgacga ggcgctggta
    11281 gcgaagatca agaagcatat tcgcgacaac accacgccgc ggcacgtccc ggcgaaggtg
    11341 ctgcaggttg gcgacatccc gcgcaccaag tcgggcaaga tcgtcgagct cgccgtacgc
    11401 cacgtcgtgc acggccggcc ggtgaagaac gtcgaggcgc ttgccaaccc cgaggcgcgg
    11461 gagcatttcc gcgaccgcgc ggagctcgcc agctgagcaa cgcggggtga gcggcggcgc
    11521 gcgcgcgctc gcgatcgtgc tcttcggcac ggtcgtcgcg ccgctcgact cggcggtgaa
    11581 cgcggccttt ccggcgatca ccgcggcctt ctccctcgaa gtcgcggcga tccagtggct
    11641 cgtcatcgcc tacatggtga cgttcagcag cctgatcatc gtcagcggcc gggtggccga
    11701 ccttttcggc caccggcgcg tgttcggctg gggcctggcc gtctcggcgc tggccttcct
    11761 cggctgcggc ctcgcgcccg gctacggcgg gctgctcgcc gcgcgcgtcg tgcagggcgt
    11821 cggcgtcgcg ttccttctcg gcgcagggcc ggcgctcgcg ctcggcgcct accccgcgac
    11881 cgagcgcacg cgcgtgctgg cgcgctacgc ggcggctttc gccatcgggc tcgcggccgg
    11941 tcctgcgctg ggcggcttgc tggtggacgc ctttggctgg cgcgcggtgt acctcgcgcg
    12001 ggtgccgatt gcgcttggcg cgctgctgct gcttgtgtac gtgccgccga cacgcatccg
    12061 gccggggcgc gccttcgaca gcgcgggcgc cctgctgctc gctgcctgga ccgggagcct
    12121 gatgctcgcg ctcacgttgc gcggcgcggt tggcgtcgga ctcggcgctg ctgcggcggc
    12181 cgctttcgcg ctgttcgtgg cacgcgaagc ccgcgcggcg gaaccgacaa tccgcgtcgc
    12241 cctgttccgc gacgcggcgt tcgccgtgcc caacctggcg tcggtggcgg tgcatttcgc
    12301 cggcttcggc atctttctcc tcgggcccta tttcttcgtg cggatcaccg gcctgtcggc
    12361 ccagggcgcc ggtctgctgc tcgcggtcgg tccggccgcc gcagccctgg gctccgcgct
    12421 cgccgggcgc atcgctcatg ccgcgggcgc gcggcacgtc gcgcttgccg gcacgctgct
    12481 caacctggcc ggggtgtcga tcaccgcgac gtgggacgcg cagaccggcg tggcggcgat
    12541 cgcgggcacg ctcgcgctgc agggcttggg catcggcctg ttccaggtcg cctacgccga
    12601 caccgtgatc gccgcgctgc cggcggagga tcgcggcgtc gccgccagcc tcgccaacct
    12661 cacgcgcacc ttcggcgtgg tgagctgcgc cgcgctgatg tcggcgctgt tcgccgccgg
    12721 cgagcgcgcg gcgctcgggg gcggcgcgcc gcccgtcgcg gcgttcgcag acggcttcgc
    12781 cttcgcgttc ggcgtcgcgg cgacgggact cacggcgctg ctcgcgctca gcttcgtgaa
    12841 gggcagggcg tggcgggatg catgagccgc gcgtttgtgg cgcgcgcgcc tttcctgtag
    12901 actccttcat ccctacacgc cgtgggtaaa tgaccaagta tgtcttcgta accggtggtg
    12961 tagtttcctc tctggggaaa ggcatcgccg ccgcctccct cgccgcgatt ctcgagtcgc
    13021 ggggcatccg cgtcaccaat atcaagctgg acccgtacat caacgtcgat cctgggacga
    13081 tgagcccgtt ccagcacggc gaggtgttcg tcaccgagga cggcgccgag accgacctcg
    13141 acctgggcca ctacgagcgc ttccagagct cgaagatgcg ccgcgccaac aacttcacca
    13201 ccggccagat ctacgagtcg gtgatcaaga aggaacgccg cggcgactac ctgggcggca
    13261 cggtgcaggt gatcccgcac atcaccgacg agatcaagca gttcatcaag aagggcgcgg
    13321 gcgacgccga cgtcgccatc atcgaggtgg gcggcaccgt cggcgacatc gagtcgctgc
    13381 cgttcctcga ggcgatccgc cagatggggc tcgacctcgg ccgccacaac gtgtgctacg
    13441 tgcacctgac gctggtgccc tacatcgcga gcgccggcga gatcaagacc aagccgacgc
    13501 agcactcggt gaaggagctg cgcgagatcg gcatccaggc cgacgcgctg ctgtgccgca
    13561 ccgaccggcc gctgcccgat gacgaaaggc gcaagatcgc gctcttctgc aacgtgcaga
    13621 aggaggccgt catctcggcc tgggacgtgg acctgatcta caagatcccg atgatgctgc
    13681 acgagcagat gctcgacgag atcgtctgcc acaagctgca gatcctggcg cgcgccgccg
    13741 acctctcgcc ctgggtcaag ctggtgcacg cgctggagca cccggagcac gagatcgacc
    13801 tcgccttcgt gggaaagtac gtcgacctgc aggattccta caagtcgctg aacgaggcgc
    13861 tgcgccacgc cggcatccac acgcgcagcc gcgtcaacgt gcattacttc gattcggaag
    13921 agatcgaggc gaagggcacg gcggtgctcg agccgatgga cgcgatcctg gtgcccggag
    13981 gcttcggcaa gcgcggcacc gagggcaaga tggccgccat ccgctacgcg cgagagaacg
    14041 ggatcccgta tctcggtatc tgcctcggca tgcagctcgc cactatcgag ttcgcgcgga
    14101 acgtctgcgg gctggagggc gccaacagca ccgaattcga tgccgagacg ccgcacccgg
    14161 tggtggccct gatcagcgag tggctcgacc gtaccgggca ggtcgagcgg cgcgacgcca
    14221 agtccgacct cggtggcacc atgcggcttg gggcgcagaa gtgcccggtc gaggcgggca
    14281 cgctcgcgca ccgcgtctac ggcgccgagg tcaacgagcg ccaccgccat cgctacgaag
    14341 tgaacaacgt gtacgtgccg cagctcgagg cgaagggata caaggtggcg gcgcgcacgc
    14401 cgagcgagcg cctgccggag atcatggagc tgcccgccca cccgttcttc atcggcgtgc
    14461 agttccaccc cgagttcacc tcgacgccgc gcaagggcca tcccctgttc aaggcgttcg
    14521 tcgaggcggc gatcgagcag cgcgcgcgcg ccaagcgcgc gccccccgga ggctcgctct
    14581 cggccgtcgc ggcatgaagc tgtgcgggtt cgaggcgggg ctcgaccagc cgttgttcct
    14641 catcgccggc ccgtgcgtcg tggaatcgcg cgagctgcag gtggacaccg cggggcgcct
    14701 gaaggagatc tgcgcccgcc tgtccatccc gttcatcttc aagtcctcct tcgacaaggc
    14761 gaatcgcagc tcgagcaagt cgtatcgcgg cctgggcatg gagcaggggc tgcgcatcct
    14821 cgccgaggtg aagcgccagg tcggcgtgcc ggtgctgacg gacgtgcacg acgaggacga
    14881 ggtgaagccg gcggccgagg tggtcgacgt gctgcagacg ccggcgttcc tgtgccggca
    14941 gaccgatttc atccgcgcgg tggctgccgc gggccggccg gtgaacatca agaagggaca
    15001 gtttctctcg ccgcaggaga tgcagaacgt ggtcgacaag gcgcgcgagg cgagcggccg
    15061 cgacaacgtg ctggtgtgcg agcgcggctt ttccttcggc tacggcaacc tggtctccga
    15121 catgcggtcc ctcgccatca tgcgcgccac gggctgcccc gtggtgttcg acgccacgca
    15181 ctcggtgcag ctgccgggcg gccagggcac ctcctcgggc ggccagcgcg agttcatccc
    15241 ggtgctggcg cgcgccgcgg tggcgagcgg catctccggg ctgttcatgg aaacgcaccc
    15301 caaccccgaa atcgcgctgt ccgacggccc gaacgcctgg ccgctcgggc tgatgccgcc
    15361 cctgctcgaa acgctcgccg cgctggaccg gctggtgaaa gaatcgggct tcgcggaaaa
    15421 ccaggtcaaa cactagccga ggcaaaccga tcccatgagc tccatcgttg acgtcgtcgc
    15481 gcgcgagata ctcgattcgc gcggcaatcc cactgtcgaa gccgacgtgc tgctcgaatc
    15541 cggctccatg ggccgcgccg cggtgccctc gggcgcctcc accggccggc gcgaggcagt
    15601 cgagctgcgc gacggggacg ccaggcgcta cggcggcaag ggcgtgctga aggcggtcga
    15661 gcacatcaat accgagattt ccgaggcgat cgtgggcctg gacgcgtccg agcagggctt
    15721 catcgacaag accctcgtcg acctcgacgg caccgagaac aagtcgcgac tcggcgccaa
    15781 cgcgctgctc gcggtgtcga tggcggtggc gaagagcgcg gccgaggagt ccgggctgcc
    15841 gctgtaccgc tacttcggcg gctccggccc gatgcagatg ccggtgccga tgatgaacgt
    15901 cgtgaatggc ggcgcgcacg ccaacaacaa cctcgacatc caggagttca tgatcgtgcc
    15961 ggtaggggcg aagagcttca gcgacgccct gcgctgcggc gcggaggtgt tccaggcgct
    16021 gaggaagctg gtcggcgagc gcggctcgtc cacggcggtc ggcgacgagg gcggcttcgc
    16081 gccggacctg cccaaccacg ccgctgccat cgagctgatt ctcgaggcga tcggcaaggc
    16141 gggctacaag ccgggcagcg acgtggcgct ggcgctcgat tgcgccgcca ccgaattcca
    16201 caagaacggg cgctacgtgc tcgcctccga gaagctggag ctcaacgcgc aggaaatgtg
    16261 cgactacctc gccgcgttcg ccgagcgcta cccgatcgtg tcgatcgagg acggcctcgc
    16321 cgaggacgac tgggacggct ggaagcggct caccgagcgc ctcggcagca agctccagct
    16381 ggtgggcgac gacctcttcg tcaccaacgt gcgcacgctg cgcgaaggcg tgaagcgcgg
    16441 cgtcgccaac gccatcctgg tgaaagtcaa ccagatcggc acgctgaccg agaccttcgc
    16501 cgccatcgag ctcgccaagc gctcgggcta cgctgcggtg atctcgcatc gctcgggcga
    16561 gaccgaggac accaccatcg ccgacatcgc ggtcggcacc aacgcgctgc agatcaagac
    16621 cggctcgctg tcgcgctcgg accgcgtcgc caagtacaac cagctgctgc gcatcgagga
    16681 ggatctgggg gattccgtga gctatcccgg ccgggaggcc ttcgcacggc tgcgctgaga
    16741 tgaagatcct gggcggcgtg cttgccgcgc tcatcctgct catccagtat ccgctgtggc
    16801 tgggcaaggg cgggtggctg cgcgtgtcgg aggtggaccg ccagctgtcc gcgcagcagg
    16861 cgaagaacgg cgcgctcgag gcgcgcaacg gcgccctgga agccgaggtg cgcgacctca
    16921 agcaggggct ggaagcgatc gaggagcgcg cgcgctacga gctgggcatg atcaagtccg
    16981 acgaggtatt cttccaagtc gtcgaaaagc ccggcaagta ggggtcgggc cgcttttccg
    17041 gcaaggagat cttatgaagc tgacgcccgc ccagagcgcg cagttcgagc gcgaaggatt
    17101 cctgttcttc cccggcctgt tcacgccacc ggaagagctg cagggcgcgc tgaaagcggc
    17161 ctgagatgaa cctgcacgcg aagctcaagg aacgcgaggc gcagggcaag ccgctgcgcc
    17221 tgggcgtgat cggcgcgggc aagttcgcca ccatgtacat cgcgcaggta ccgcgcaccc
    17281 caggcgtgca tctggtcggc atcgccgacc tgaacccggc gtatgcgaag gccaacctcg
    17341 agcgcaccgg ctgggcgccc gagcacttcg gcgcatcgtc gctcgacgcc gcggcgaagg
    17401 aacggcgcac gcacctgggc gaggactggc gcgcgctggt ggcgcacccg gcgatcgaca
    17461 tcgtcatcga ggccacgggc aacccgcttg ccgccgtgga gcacgcgctg gaggcgttcc
    17521 gcaacggcaa gcacgtggtg atggtcacgg tagaggccga cgcgttctgc gggccgctcc
    17581 ttgcgcagaa ggcggctgcg gcgggcgtgg tgtacagcct cgcctacggc gaccagcccg
    17641 cgctcatctg cgacctcgtc gactgggcgc gcgccgcggg cttcccggtg gtggcggcgg
    17701 ggcgcgggca caaatggcag gcgcacttcg cgcagtccac gccggagacg gtgtggggct
    17761 attacggact gaccccagag caggccaagg tcggcgggct caacccgaag atgttcaatt
    17821 ccttcctcga cggatcgaag cccgcgatcg agaccagcgc cgtgtgcaac gcgaccggcc
    17881 tcacgcccgc gccgggcggg ctcgcgtacc cggccgggcg catcgaccag attccgaacc
    17941 tgatgcgccc gcgcgccgaa ggcggtgtgc tgcaccacaa gggccaggtc gaagtggttt
    18001 cctcgctgca gccggacggc acgccggtcg actacgacat ccgcttcggg gtattcgtgt
    18061 cgttcgaggg ggagagcgac tacatccgcc gctgcttcgc cgagtacggc gtaaagaccg
    18121 acgacagcgg ccgctacgcc tgcctgtaca agcgctggca cctgatcggg ctcgaagtcg
    18181 gcatttcggt cgcttcggtg ggactgcgcg gcgagcccac cggctgcgcc acgggctttc
    18241 gcgccgacgc cgtggcgacc gccaagcgca cgctcaaggc cggagaggtg ctggacggcg
    18301 agggcggcta cacggtggtc ggccggctca tgccggccgc ggagtcgctg gcgcaaggct
    18361 gcctgccttt gggcctggcc cacggctgga agctgctcaa gcccgtcgcc gcggggcagc
    18421 cgctgcgctg gagcgacgtc gccttcgacg ccgaggtggc tgcggtcaag ctgcgccgcg
    18481 agatggaggc cgcgtgccgc gccgcggagc gccatgccgc ctgagggcgc gccgcgcttc
    18541 ctcaccaagt tcgcgttccg gatccgcacc cgcagcggca cgccggtgga aaacctcatg
    18601 ctgcaggccg ccgaccgcgc gcaggccgaa gcccgcatcc agcagatgta cccctactgc
    18661 gagatcgtcg aatgccgcga gctgacggcg acgccgcgtg acgagacggc gagcctcgac
    18721 agcatcctct cgatcatctc gcgcgaagag ggcgagccgc gctaggcggc gggcggcggc
    18781 gggcgccgct cgatgaagcc cgtggcgatg agctcctcga cgagcgcgtc gtagtcctgc
    18841 gcgccccggc tctcgggggc atgcgcgaac acgtccttgc acgaagccgg gctttccgcc
    18901 agtgcgacgt tctccgagat gcgcgtgccg cagatgtcca tgccgaagcg cgcgtgcagc
    18961 tcgccgacga tctggtgcga gagcttgcgg cgcgtgtcga aacgggtgac cacgtagcgc
    19021 cgcgcgacgc gcttcttcag cacgtgctcg agcgccttca gcgtcttctc gcactgctgc
    19081 acgcccttca gcgccaggta atccgcggaa accgggatca gcacccggtc ggcggcgaag
    19141 atcgccgaca gcgacagcac gccgagcacc gggcagcagt cgatgacgat cggccggccg
    19201 gtgttcaggt tctcgcggat gatgccgagg ttgaggcggt tgaggacgtt ggcacccttg
    19261 ccgtagtggg tgtcgacctt ggacagctcg acgtgcgccg agatcgcgag ccagccgccg
    19321 gtcgccggac ggatgagctg cgtcagcggc tttgcttgct ggtagaagcc gaaaatcgaa
    19381 tcgtcggagc gctcgacggc cgcgccgcag acgtaggaca ggtgcgcctg ggggtcgagg
    19441 tcgatcgaca gcgggttgaa gccgcggcgg gcgagggccg cggtgaggtt gagcgaggtc
    19501 gtggtcttgc cgaccccgcc cttctgattg aataccgcga tcttggccat gcggcgttgc
    19561 ccctgggccg accctaccta cggggcgcgc cgcctgtcaa ttaactagtt ccgcggtcct
    19621 tgaagacttc cttggcgcag cggaacgcca ccaccgccga gggcaccccg cagtagatcg
    19681 cgacctgcag gaatacctcg cggatctgct ccttggagag gccgttgttg agcgcgccgt
    19741 tgatgtgcag cttcagctcg tgctccttgc ccagcgccga aagcatggcg aggttgagca
    19801 tggagcgcgt cttgcgatcc aggccgggcc ggttccagat gtcgttccag caataggtgt
    19861 tgagaaggtc gacgaagggc ttgctgaagt cgtcgacccc cgccatcgcc ttgtcgacgt
    19921 aggcggcgcc gagcacctcg cgccgcgtct tcagtccgtc gtcgaaccgt tctttctgac
    19981 tgcgcttgtc catcgatgtc tccgttctag atgatctctt cgtagcggat cccggcgcgc
    20041 cgcagcaggg cgtcgaacgg cgggccgccg gggcgcgggt ccttgttgaa agcctgcgag
    20101 cgtgccggcg tctcgtcgag cacgcgctgc gagatgggga ggttctcgcc gcgcatcgcg
    20161 tcggtcgcga cctggatctc gcaggcgcgc tgcaggcccc agtaggtgag gtaggcctgg
    20221 ggaatgccgg ggcaggcgac gagcaggccg tggttgcgca ggatcaggat cgccttgtcg
    20281 ccgatgctct tcacgaggcg cgcctgctcc gacagatcgg tggtgacgcc ctcgaagtcg
    20341 tggtacgcca cctgtccata caggagtgcg ctgtagaaat tgtcgaagcg caggccgtcc
    20401 ttcttcgacg ccaccgcgct gccggcggtg ctgtgcgtgt ggatgatgca gtgcgcgtct
    20461 tcgcgcgcgc cgtggatcgc gctgtggatg acgaagccgg ccacgttcac cgggtacttc
    20521 gagccgtcca tcacctcgcc cttgaggttg atcttgacga ggttggtggc cgtgacctcg
    20581 ctgtagttga ggccgtaggg attgatcagg tagtggcgct cgccgcccgg cacgcgcgcg
    20641 gtgatgtgat tgaagatcga ctcgctccag cccagccagt cgaacgcgcg gtagagggcg
    20701 gccagctcca gccggacgac ccattcgtcg tcgtcgatcg cagcgggctt gagccgcagc
    20761 agcttgtcgc tcaggtccat gtcggcctcc ttggcaccga ctatagccgc tttcgcgcac
    20821 gcagcaacag cgtccccgcg atcgccatgt tgagcaggtt ccagccgatc ccgttcagga
    20881 acgcggcccg gtacgagccc gtcaggtcga agatcgcgcc cgacatccag ccacccaggg
    20941 ccatgccgaa aagcgtcgag gtcagcacga tgctgacgaa ggtgccggcg acccgcggcg
    21001 cgaagtattc gcgcacgatg atggcgtagg agggcacgat gccgccctgg aacaggccga
    21061 acatggcgga caccacgtac agcgacgtca ggtcgtcggc gccgagatac aggccgagcg
    21121 cgagcatctg cagcgtggac ccgaggagca gcgtgcgcag gcccccgatg cggtcgcaga
    21181 tccagcccga cagcaggcgg ctgacgatgc cgaagccgag catcagcgac agcatctccg
    21241 cgccgcgcgc tgcggcgaag ccgaggtcgc cgcacagcgc cacgatgtgc acttgcggca
    21301 tggacatcgc cacgcagcag gcgacgccgg cggtgcagag caggccgagg accagtcccg
    21361 gccgcaggcc gagcgactgc ggcgcgggcg cgtgcccggt accgtccgcc gaggtgacga
    21421 ccagcacggg cggccgccgt cgcagcacga gcgcgagcgg cagcatggcc gcgatgcaga
    21481 agacgccaac gcccatgtag gtctggcgcc agccgacggt gtcgaagtaa tgctgcacca
    21541 ccgggggcca cagcgcgccg gcgaggtagt tgccgctcgc gcagatgccg accgcgatgc
    21601 cgcggcggcg atggaaccag agcgaggtgt cggcgaccag cggcgcgaac gtcgccgagc
    21661 agccgagaaa gccgatcaac aggccctgcg ccagcgcgaa ctgccacagg ctgccggcga
    21721 accccgcgac gacgaagccc gcgcccaggc acacggtgcc gatgacgacc ggcaccagca
    21781 ccccgaagcg gtcggccagc cgtcccatga cgatcccgcc caggccgaag ccgatcatgg
    21841 tgagcgtata gggcagggac gcgtcggagc ggtccacgcc gtattccgcc tgcaccagcg
    21901 gcagggcgac ggtgacgatg tacatgcccg cgccgccgag cgtcatcagc gcgagcgagg
    21961 ccgccaggcg cagccaggcg tacggcgatt ccgcttcgga gctcaagggc ggctaggaag
    22021 cgggcgcgaa gaccagaaag tactggtagg gcaggaacga atgctcgccg gccacgcggt
    22081 agcctgccgc cttcatctcc gctttcacgg tttcgggcgc aatgcgcccg gcgggcggcg
    22141 ggcccacggg cgagtcgagc ttgaaatcga cgatggcgat gcggccgccg gaacgcagcg
    22201 tgccgcgcag gcggctgaag taggcgctgc gctcgccgat gtggtggtag acgtcgacca
    22261 gcaggatgag atcgacctgc gccggcaggc gcgggtcgtc cggttcccct ttgaccggca
    22321 cgatattgcg caggccttcg cgcttcgctc gcgcctcgag gtagcgcacc atgtccggct
    22381 cgatgtcgac cgcgtagatc cggccgccgg gcagcatgtt ggcaagacgc gcggaaaagt
    22441 agcccgtgcc ggcaccgagg tcggcaacga gcgcgccggg ctttatcgcg agggcctgga
    22501 tcacctcatg cggcttttgc caggcgtcgc gctgcgggtc gtcgaagatg tgcgaccatt
    22561 tctcggcgtc gcggaacgag tgctgatgcg tgtgcgggga ctgcgcggtg gcgcaaccgg
    22621 cggccagcaa gaggacgaag gcggcaactg acgatcgcat gatggcaagt ttatccggtt
    22681 tgcggtccgg cacgacgctg gtcctgctgc acgggcttgc cagcaacgcg acgcgctggt
    22741 ggcatttcgc cgcgcacgcg cagctgccgg ggctgaagct ccttcggcct accctgcgcg
    22801 gaggtgccgg ctcgagcgac cgcgggcgca tcgacatgcg cgtatggtgc gacgacctcg
    22861 tgcggctgct cgatgcggaa aactgcgagc gcgccgtggt tggcggccac tgcctcggcg
    22921 ccaacatcgc gttgcacttt gcgtcgcgcc accccgggcg cacggcgggc ctggtgctga
    22981 tcgagccgat gccgccgcag gcgctggcgg gcagcatgcg gcggattcgc ttcttcaggc
    23041 ggccgatcct cgccctggcc gccgtcgtgc gcgcgctcaa tgcgctcggc atccgccggg
    23101 caggcttcga gccgctcgat ctcgagcagt gggaccgcgc tctggcacgc gacgagggcg
    23161 acctggggcg gtacgcctcg ccgctatccg accttaggct cgcgcccgtg gcggcctacc
    23221 tgcaaagcct cgccgctctg ggcgagccgc tgccgccgct cgcctcgatc cgcgccccgg
    23281 cgctggtgct gctttcgcgc agcagcacga tgaccgatcc ggtacggacg cgcgccattc
    23341 tcgaagagtt gccccgtgtc gaattcaaag tgctggaggc cgagcactgg attccgacag
    23401 agcagccgga agacatgcgg atggcgatcg aggcgtggct cgctgcgaac gcggcgagcc
    23461 tcgcacaatg agagtggctc cccgaccagg gctcgaacct gggacctgcg gattaacagt
    23521 ccgtcgctct accaactgag ctatcgggga tccggaaaga ccgcgattct accggattac
    23581 cccggcgatg gcctcggcga cggtgtcgat gttgcccgaa ttgagcgccg cgacgcacac
    23641 gcgcccggtg tcgatggcgt agatcgaatg ctccttgcgc agccgctcga cctggtcctt
    23701 ggtcagaccg gtgtacgaga acaggccgcg ctgcttgagc acgaacgaga aatcctttcc
    23761 cgagcgcttc tgcacgccgt cggcgagcgc cttgcgcatc gccttgatgc ggtcgcgcat
    23821 cgccgctagc tccttttccc acagggcgcg aagctcgggc gtgttgagca cgcgcgccac
    23881 gatgtcgccg ccgtgcgtcg gcgggttgga ataattggtg cgcaccacgc gcttcacgtg
    23941 cgagagcgcg cgcgccgctt cgtcggcgct gcccgccacc agcgtgaacg cgccgacccg
    24001 ctcgccatag agcgacagcg acttggagaa cgagctcgac aggaagatcg gcgacatggc
    24061 ggcggcgaac aggcgcggcg cataggcgtc cgcgtcgatg ccttcggcga acccctggta
    24121 cgcgaggtcg aggaacggca gcagtgcccg tgcctgcacg atcgcgagca ccttgcgcca
    24181 ctgctcctgg ctgaggtcca cgcccgtcgg gttgtggcag caggaatgca gcacgacgag
    24241 ggtgccggcg ggcagcgctt ccagcgcctg gcacatcgcc tcgaacttca ggccgcgggt
    24301 cgccgcgtcg tagtagggat aggcctgcac cttgaacccg gcggcctcga acagcgcgcg
    24361 gtggttctcc cagctcggct cgctgatcca gacctgcgcc gcgggcgcga gcagcttcag
    24421 gaagtcggcg ccgatcttca gtccgccggt gccgccgatc gcctgcaccg tgacgacgcg
    24481 cttttccttc agcaccggcg catccgcgcc gaacaccagc gcctgcaccg cctggtcgta
    24541 agcctgcagg ccgtcgatcg gcagatagga cttgggcgcg gcggtctgca cgcgctgctg
    24601 ctccgcgcgc tgcacgcact cgagcagcgg caccttgccc ttgtcgtcgt agtacacgcc
    24661 gacgccgagg ttgaccttgc gcggattctt gtcggcgacg tagagctcgg tcacgcccag
    24721 gatcggatcg cggggcgcca tcggcacgga ggcgaggagg gagggggcgt gcggcgcgtt
    24781 catcgtgcta ggatctttcg gctttggcag ccgaaattct acccgtggcg ctggctttcc
    24841 ccaacagccc gttccgcttg caccagccgt tcgcgcccgc gggcgaccag cccgaggcga
    24901 tcgaggcgct gaccgagggc gtgcgcgacg gcctcgcgtt ccagacgctg ctcggcgtca
    24961 ccggctccgg caagacctac accatggcgc acgtgatcgc gcgcctcggc cgcccggcgc
    25021 tggtgctggc gccgaacaag acgctggcgg cccagctcta ctcggagttc cgcgagttct
    25081 tcccggagaa cgcggtcgag tacttcgtct cgtactacga ctactaccag cccgaggcct
    25141 acgtcccggc gcgcgacctc tacatcgaga aggactcctc gatcaacgag cacatcgagc
    25201 agatgcgcct gtcggcgacc aagtcgctga tggagcgccg cgacgcggtg atcgtggcca
    25261 cagtctcggc gatctacggc atcggcgatc cgtccgagta ccacggcatg atcctgcacc
    25321 tgcgccaggg cgaaaaggcg acgcagcgcg aggtgatcgc gcgcctggtg gcgatgcagt
    25381 acgagcgcaa cgagctcgac ttccgccgcg gcacgttccg cgtgcgcggc gacgtgatcg
    25441 acgtctttcc cgccgagcat gccgagcacg cgctgcgcct gtccctcgcc gaggacgaga
    25501 tcgagcggct gcagctgttc gatccgctca ccggccgggt actgcacggc ctgacgcgct
    25561 tcaccgtcta cccgtcgagc cactacgtga cgcctcgcga gaccacgctg cgcgccatcg
    25621 aggcgatcaa ggcggagctg cgcgagcgca tcgagttctt ccagaaggcc gggcgcctgg
    25681 tggaggcgca gaggatcgag cagcgcaccc gtttcgacct ggagatgctc gtcgagctcg
    25741 gcttctgcaa gggtatcgag aactattcgc gccacctctc cggccgcaag gcgggcgagc
    25801 cgccgccgac ccttatcgac tatctgccgc ccgatgcgct gatgatcatc gacgagagcc
    25861 acgtcaccat cggccagctg ggcggcatgt acaagggcga tcgctcgcgc aaggagaacc
    25921 tcgtcgagta cggctttcgg ctgccctcgg cgctcgataa ccggccgctg cgcttcgacg
    25981 agttcgaagg cgtggtgcgc cagacggtgt tcgtgtcggc caccccggcc gactacgagg
    26041 cgcagaagtc cgcgcaggtg gtcgagcagg tggtgcgccc gaccgggctg gtggacccgg
    26101 cggtcgaggt gcgcccggcg tcgagccagg tcgacgacct gctgtccgag atccgcctgc
    26161 gaagcgcgaa gcgcgagcgc gtgctcgtga ccacgctcac caagcgcatg gccgaggagc
    26221 tgaccgagta tctatccgag cacggcgtga aggtgcgcta cctgcattcg gaggtggaca
    26281 ccgtggagcg cgtggagatc atccgcgacc tgcgcctggg cgagttcgac gtcctcgtgg
    26341 gcatcaacct gctgcgcgag gggctcgacc tgcccgaagt gtctctggtg gcgatcctcg
    26401 atgcggacaa ggagggcttc ctgcgctccg agcgctcgct gatccagacc atgggccgcg
    26461 ccgcgcgcca tctgaacggg cgcgcgatcc tgtatgccga tgtcgtcacc gaatcgatgc
    26521 agcgcgccct cggcgagacc gagcgccggc ggcgcaagca actgacgtgg aatgcgtcgc
    26581 acggcatcac gcccaggggc gtgagcaagc gcatcaagga cctgatcgac ggcgtgtacg
    26641 acgccgagga cgcggccgcc gagctgcggg ctgcgcagga cgaagcgcgc tacgaggcga
    26701 tgagcgaaaa gcaggtggcg cgcgagatcc ggcgcctgga aaaggacatg atcggccacg
    26761 cgcgcaacct cgagttcgag aaggcggcgc agctgcgcga ccagctcgcc gccctcaaga
    26821 agcgcgtgtt cggcgtcgag ctcgcgcccg agtagccgcc cgcgtcagtt cttcgtctcg
    26881 acctggacca ggggctcgtc ctcggccgcc gcgggacgcg aacgctcgcg ccgcggccgg
    26941 ccgagcggca cggcttccgg ctcggccagt gccgcgggca cgcggtcggg ccgcgtttcg
    27001 accatctgca ggccggcgct ttccagcact tcccgcggat cgactttcgc catggccggc
    27061 ggtgacgcgg gtatctcgcg ctcgggcgcc ggctgcggcc gcggttcgcg catctcgcgc
    27121 tcgggcgccg gctgcggacg cggttcgcgc atctcgcgct cgggctgcgg ttccggttgc
    27181 acctgcgccg cgcgctgttc ggcctcggca ttcagccgcg cctcctcgcg catctgcgcc
    27241 tcgtgcattt cccgctcgcg ggcacgcaat tccttctcgt ccggttcccg cgccagatag
    27301 ctcggaatgc ccggatccgc cggcagcgaa ccggcctgct ccggcgcgcg accgccctgg
    27361 ccctggccgt cgcgggaccc gtcgggacgc cgatcgcgat tgcggtcgcg gccgcggccg
    27421 cgccggccgc gacgatgttc ttcgccgtcg cgggtgccgg taagtgcact ctccgcaggg
    27481 cgggggccct gaggctgctg tccgggctgg ggctgcggcg gccgctgctg ctggtgcggc
    27541 ggccgctgct gcgagtgctg cgggcgctgg tctggccggc gctcgccgc
//
LOCUS       DBNHKE010000225         5692 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_42015, whole genome shotgun sequence.
ACCESSION   DBNHKE010000225 DBNHKE010000000
VERSION     DBNHKE010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_42015"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="AC16O9_15275"
     CDS             <1..852
                     /locus_tag="AC16O9_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6518015.1"
                     /translation="FDAIFFGAVGAPDVPDHVSLWGLRLPICQGFDQYANVRPSRVLP
                     GVTSPLANAKDIDWVIIRENSEGEYSGSGGRVHTGLPEEVATETSVFTRAGVERIHRF
                     AFELARKRPRKMLTLVTKSNAQRHGMVLWDQIFYEVAEKYPDVKTDRILVDAMTTRMV
                     LKPASLDTIVASNLHADILSDLAAALSGSLGIAPTANLNPERKFPSMFEPIHGSAFDI
                     TGKGVANPIATFWTAAMMLEHLGEPKAAARLMKAIERTTEEQVFTPDLGGVHDTRAVT
                     SAVKRAL"
     gene            869..1744
                     /locus_tag="AC16O9_15280"
     CDS             869..1744
                     /locus_tag="AC16O9_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6518016.1"
                     /translation="MLHADSARNRLTGIGLISLTYMLFTLLDGSAKWLVQSVPVIVVV
                     WLRFLSQTVLASALLFPLRGAALVRTRHLRWHVARGLMFCAMTGINFWALQYLTLTVT
                     ASIFFTVPILIALMSAPLLGERMDARRWAAILIGLAGVLVIVRPGSAAFHPAMLLSLV
                     NAVLYAAFNLMTRKLAAYDSPETIQFLPALVASVVLAPFALVLWDSPTGWFEWTLLCL
                     MGVFGGTGHYLLAVAHRYAPASTLAPFLYQQILYMALFGYLVFGTVPDPAVWIGAAIV
                     IASGLYLFARERRAR"
     gene            1793..3199
                     /locus_tag="AC16O9_15285"
     CDS             1793..3199
                     /locus_tag="AC16O9_15285"
                     /inference="COORDINATES: protein motif:HMM:NF039580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6518017.1"
                     /translation="MAESVSIPLWLAVAGALLAAWALYEHVLMPALRWLVMHPANQVI
                     DEVGKRLRIGIRPFQRTRRQALIHRLLTDPKVQQAAEEYAREHNVSVTAALHRVERYA
                     RETVPAFNAYLYFRIGYWLGKRLAQLLYRVRLGYVDAENLQRVAPDSTVVFVMNHRSN
                     MDYVLAGYLAADQTALSYAVGEWARIWPLSALIRWMGAYFVRRNSRDELYRRVLERYI
                     VMATEAGVPQAVFPEGGLTRDGRMREPKLGAIDYMMRGFRVDGERDLVFVPLGINYDR
                     VLEDRTLLRDLDPDAPRRGRLAALGTTLRFFLRNFWLMVRSEWHRFGYACVNFGGPVS
                     MREYCEARNLDFHRLHGDERKRAMADLGSHLMQTVGRIVPVLPVPLIATVFVRQGDTA
                     ISELELKAEVGQLVEALRARGAHVYVPRKDFDYAVHVGLRMLTLRRLVGERDGLYYAR
                     SEELPLLRYYANSIAHLL"
     gene            complement(3365..3631)
                     /locus_tag="AC16O9_15290"
     CDS             complement(3365..3631)
                     /locus_tag="AC16O9_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518018.1"
                     /translation="MRLAVTLLFAALMLAQVPASAQERGWRGQQGQKQGFAPRERERE
                     GREALRRERSERREDRREQRFTPEEREKLRQDLLDANRDLRRRR"
     gene            3683..4405
                     /gene="ompR"
                     /locus_tag="AC16O9_15295"
     CDS             3683..4405
                     /gene="ompR"
                     /locus_tag="AC16O9_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmolarity response regulator transcription
                     factor OmpR"
                     /protein_id="HIR6518019.1"
                     /translation="MAELKTRILVVDDDQRLRDLLTRYLGEQKFEVRAVADAPAMDKQ
                     LARERYDLLVLDLMLPGEDGLAICRRLRAAGGAPAIIMLTAKGDEVDRIVGLEMSADD
                     YLPKPFNPRELVARINAVLRRKPRGGVPGAPGVEDGIYRFGAYEFNPGTRELKKDGKP
                     VALTTGEYSVLKVLVERPRQPLSRDQLMELARGREYEVFDRAIDVQISRLRKLLEDDP
                     SHPRYIQTVWGHGYVFVPDGQK"
     gene            4406..5692
                     /locus_tag="AC16O9_15300"
     CDS             4406..5692
                     /locus_tag="AC16O9_15300"
                     /inference="COORDINATES: protein motif:HMM:NF027838.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6518020.1"
                     /translation="MTLFWRSFLLIAALIVVSVVASFQIQRVYEREPRARELAQQAVS
                     SVNLTRAALVNADPFRRRALLIELNEREGLRVYPVTNAETIEPLPRDPLLERVVANVR
                     GDLGGATRFANARDGMEGFWVSFFIDEDEFWVMLPQERFDPELGLGWLGWGAALLALA
                     LLGASLIAATLSRPLRALAGAARRVGHGEKPAPLPESGPRELATVSTAFNRMARDLEA
                     LERERAMVLAGISHDVRTPLSRLRLALEMSGADAAAAQGMSADIDEMDQVIGQFLEFA
                     RGENEAKTPADLGALLAEIGEHYARLGKDVKVGAAPIAPFAFARMALRRAVSNLIDNA
                     LRYAGEPVEVEARREGAEVVIEVLDRGPGIPAAETERLKQPFTRLDPARSGRGGAGLG
                     LAIVERVARAHGGRLALAARPGGGLAARLTLSAAAA"
BASE COUNT          811 a         2025 c         1957 g          899 t
ORIGIN      
        1 ttcgacgcca tcttcttcgg cgcagtcggc gcgccggacg tgcccgacca tgtgtcgctg
       61 tggggcctca ggctgccgat ctgccagggc ttcgaccagt acgcgaacgt gcgcccttcg
      121 cgcgtgctgc cgggggtcac cagccccctc gccaacgcga aggacatcga ctgggtgatc
      181 atccgcgaga actccgaggg cgagtactcg ggctcgggcg ggcgcgtgca taccgggctg
      241 ccggaggaag tcgccaccga gacctcggtg ttcacgcgcg cgggcgtcga gcgcatccac
      301 cgtttcgcct tcgagctggc gagaaagcga ccgcgcaaga tgctgacgct ggtcaccaag
      361 tccaacgcgc agcgccacgg catggtgctg tgggaccaga tcttctacga ggtggcggag
      421 aaatacccgg acgtgaagac cgaccgcatc ctggtggacg ccatgaccac gcgcatggtg
      481 ctcaagcccg cctcgctcga caccatcgtc gcgtcgaacc tgcacgccga catcctctcg
      541 gatctcgccg ccgcgctctc cgggtcgctc ggcatcgcgc ccacggcgaa cctcaacccg
      601 gagaggaagt ttccctcgat gttcgagccg atccacggct cggccttcga catcaccggc
      661 aagggcgtcg ccaacccgat cgccaccttc tggaccgcgg cgatgatgct cgagcacctg
      721 ggcgagccga aggccgccgc acggctgatg aaggcgatcg agcgcaccac cgaggagcag
      781 gtgttcacgc cggacctcgg cggcgtgcac gacacgcgcg cggtgacgag cgcggtcaag
      841 cgcgccctgt aggcgtccgg ccccggacgt gctgcacgcg gacagcgcga ggaaccgtct
      901 caccggcatc ggcctgatct cgctcaccta catgctgttc acgctgctcg acggcagcgc
      961 gaagtggctg gtgcagagcg tgcccgtgat cgtggtggtg tggctgaggt ttctgtcgca
     1021 gacggtgctc gcctcggcgc tgctgttccc gctgcgcggc gcggcgctcg tgcgcacgcg
     1081 ccacctgcgc tggcacgtcg cgcgcggcct gatgttctgc gccatgaccg gcatcaactt
     1141 ctgggcgctg caatacctca cgctcaccgt cacggcgtcg atcttcttca cggtgccgat
     1201 cctgatcgcg ctgatgagcg cgccgctcct cggcgagcgc atggacgcgc gccgttgggc
     1261 ggcgatcctc atcggccttg cgggcgtgct ggtgatcgtg cgcccgggct cggcggcgtt
     1321 tcatccggca atgctgctct cgctggtcaa cgccgtgctc tacgccgcct tcaacctgat
     1381 gacgcgcaag ctcgcggcct acgactctcc ggagaccatc cagttcctgc ccgcgctggt
     1441 ggcgagcgtg gtgctggcgc cgttcgccct cgtcctgtgg gactcgccga ccggctggtt
     1501 cgaatggacg ctgctgtgcc tgatgggcgt gttcggcggc accggccact atctgctcgc
     1561 cgtcgcgcac cgctacgcgc cggcgagtac gctcgcgccg tttctctacc agcaaatcct
     1621 gtacatggcg ctgttcggct acctggtgtt cggcaccgtt cccgatcccg cggtgtggat
     1681 cggcgccgcc atcgtcatcg ccagcggcct gtatctgttc gcgcgcgagc gccgcgcccg
     1741 ctaggcaggc gttgcacgcc ggtaacggct agactcttcg cagggaggca gggtggcgga
     1801 gagcgtttcg attcctctgt ggctggcggt cgcgggcgcg ctgctcgcgg cctgggcgct
     1861 ctacgagcac gtgctgatgc cggccctgcg ctggctggtg atgcacccgg ccaaccaggt
     1921 gatcgacgag gtcggcaagc ggctgcgcat cggcatccgc ccgttccagc gcacgcgccg
     1981 gcaggcgctg atccaccgct tgctcaccga tcccaaggtg cagcaggccg ccgaggaata
     2041 cgcccgcgag cacaatgtgt ccgtcaccgc tgcgctgcac cgcgtcgagc gctatgcgcg
     2101 cgagacggtg ccggcattca acgcctacct gtacttccgc atcggctact ggctcggcaa
     2161 gcgcctggcg cagctgctct accgcgtgcg cctgggctac gtcgacgcgg agaacctgca
     2221 gcgtgtggcg ccggactcta ccgtggtgtt cgtgatgaac caccgctcga acatggacta
     2281 cgtgctggcg ggctacctcg ccgccgacca gacggcgctg tcctacgcgg tgggcgagtg
     2341 ggcgcggatc tggccgcttt cggcgctgat ccgctggatg ggcgcgtact tcgtgcgccg
     2401 caactcgcgc gacgagctgt accggcgcgt gctcgagcgc tacatcgtga tggccaccga
     2461 agcgggcgtg ccgcaggccg tgttcccgga aggagggctc acgcgcgacg ggcgcatgcg
     2521 cgagcccaag ctcggcgcca tcgactacat gatgcgcggc ttccgcgtcg acggcgagcg
     2581 cgacctggtg ttcgtgccgc tcggcatcaa ctacgaccgt gtactcgagg accgcacgct
     2641 gctgcgcgac ctggaccccg acgcgccgcg ccgcgggcgc ctcgcggcgc tcggcaccac
     2701 gctgcgcttt ttcctgcgca acttctggct catggtgcgc agcgagtggc accgctttgg
     2761 ctacgcctgc gtgaacttcg gcggcccggt atccatgcgc gagtactgcg aggcgcgcaa
     2821 cctggacttc caccgcctgc acggcgacga gcgcaagcgc gcgatggccg acctcggcag
     2881 ccacctgatg cagaccgtgg ggcgcatcgt gccggtgctg ccggtgccgc tcattgccac
     2941 cgtgttcgtg cgccaaggcg atacggcgat ctccgagctg gagctgaagg ccgaggtcgg
     3001 gcagctggtc gaggcgctcc gcgcacgcgg cgcccacgtc tacgtgccgc gcaaggactt
     3061 cgactacgcc gtgcacgtgg ggctgcgcat gctcaccctg cgccgcctgg tgggcgagcg
     3121 cgacggcctg tactacgcgc gcagcgagga gctgccgctc ctgcgctact acgccaactc
     3181 catcgcccac ctgctttaaa tagggacaga cctcattttt ccgggggacg aagcaagccc
     3241 ccgcgcgggt ttgcttccca tacatctcgg ctttttccgg ccgggagatt catccggccg
     3301 gaaaaagccg agaccatggg aaattcgacg aaggcaccgc cggaaaaatg aggtctgtcc
     3361 ccatttaccg acgccggcgc aggtcgcggt tggcgtcgag caggtcctgg cgcagctttt
     3421 cgcgctcttc gggggtgaag cgctgctcgc ggcggtcttc gcggcgctcg ctgcgctcgc
     3481 ggcgcagcgc ctcgcggccc tcgcgctcgc gctcgcgcgg cgcgaagccc tgcttctggc
     3541 cctgttgccc gcgccagccc cgttcctgcg cggaagcagg aacctgcgcc agcatcaatg
     3601 ccgcgaacag cagcgtaacg gccagtctca tgggcgcagt gtaaggtgaa tgcgccacgg
     3661 attctgtaac attccgtaac ccatggccga gctcaagacc cgcatcctcg tcgtcgacga
     3721 cgaccagcgc ctgcgcgacc tgctcacgcg ctacctcggc gagcagaagt tcgaggtgcg
     3781 cgcggtcgcc gacgcgccgg ccatggacaa gcagctcgcg cgcgagcgct acgacctgct
     3841 ggtgctcgac ctgatgctgc cgggcgagga cggcctcgcc atctgccggc gcctgcgcgc
     3901 cgcgggcggc gcccccgcga tcatcatgct caccgccaag ggcgacgaag tggaccgcat
     3961 cgtcggcctg gagatgagcg ccgacgacta cctgcccaag cccttcaacc cgcgggaact
     4021 ggtggcacgc atcaacgccg tgctgcgcag gaagccccgg ggcggcgtgc cgggcgcgcc
     4081 cggcgtggag gacggcatct atcgcttcgg cgcctacgaa ttcaatcccg gcacgcgcga
     4141 gctgaagaag gacggcaagc ccgtcgcgct caccacgggc gaatactcgg tcctcaaggt
     4201 gctggtggag cgaccgcgcc agccgctgtc gcgcgaccag ctgatggagc tcgcgcgcgg
     4261 gcgcgagtac gaggtattcg accgcgccat cgacgtgcag atctcgcgcc tgcgcaagct
     4321 ccttgaggac gatccctcgc acccgcgcta catccagacg gtgtggggcc acggctacgt
     4381 cttcgtgccc gacggccaga agtagatgac gctgttctgg cggtcattcc tgctgatcgc
     4441 cgcgctgatc gtcgtctcgg tcgtcgcctc gttccagatc cagcgcgtct acgagcggga
     4501 gccgcgcgcg cgcgagctcg cgcagcaagc ggtgagctcg gtcaacctga cgcgcgccgc
     4561 gctggtcaac gccgatccgt tccggcgccg cgcgctgctc atcgagctga acgagcgcga
     4621 gggcctgcgc gtgtacccgg tcaccaacgc cgagacgatc gagccgctgc cccgcgaccc
     4681 cctgctcgag cgcgtggtcg cgaacgtgcg cggcgacctc ggcggcgcca cgcgcttcgc
     4741 caacgcacgc gacggcatgg aaggcttctg ggtcagcttc ttcatcgacg aggacgagtt
     4801 ctgggtcatg ctgccgcagg agcgcttcga ccccgagctc gggctcggct ggctcggctg
     4861 gggcgcggcg ctcctcgcgc tcgcgctgct cggcgcctcg ctcatcgccg cgacgctgag
     4921 ccggccgttg cgcgcgctcg ccggcgccgc gcgccgcgtc ggccacggcg agaagccggc
     4981 gccgctcccc gagtccgggc cgcgcgagct ggcgacggtt tccacggcgt tcaaccgcat
     5041 ggcgcgcgac ctcgaggcgc tggagcgcga gcgcgccatg gtgctggccg gcatctcgca
     5101 cgatgtgcgc acgccgctgt cgcgcctgcg cctggcgctg gagatgagcg gcgccgacgc
     5161 ggcggccgcg cagggcatga gcgccgacat cgacgaaatg gaccaggtga tcggccagtt
     5221 cctggagttc gcgcgcggcg agaatgaagc gaagacgccg gccgatctcg gcgcgctgct
     5281 cgccgagatc ggcgagcact acgccagact cggcaaggac gtgaaggtgg gcgccgcgcc
     5341 gatcgcgccc tttgccttcg cgcgcatggc gctgcggcgc gcggtgtcca acctgatcga
     5401 caacgcgctg cgctacgcgg gcgagccggt cgaggtcgag gcgcggcgcg aaggcgccga
     5461 ggtggtgatc gaggtgctcg atcgcggccc gggcattccc gccgccgaaa ccgagcgcct
     5521 gaagcagcca ttcacgcggc tcgaccccgc caggagcggg cgcggcggcg ccgggctcgg
     5581 cctggccatc gtcgagcgcg tggcacgcgc gcacggcggc cgtctcgcgc tcgcggcgcg
     5641 gcccggcggc gggctcgcgg cgcggctcac cttgtcggcg gccgcagcgt ga
//
LOCUS       DBNHKE010000226        10150 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_95331, whole genome shotgun sequence.
ACCESSION   DBNHKE010000226 DBNHKE010000000
VERSION     DBNHKE010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_95331"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..571
                     /locus_tag="AC16O9_15305"
     CDS             95..571
                     /locus_tag="AC16O9_15305"
                     /inference="COORDINATES: protein motif:HMM:NF014012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR6518021.1"
                     /translation="MLHFAILFVLFYAFWILMSGFFTPFLLAAGAATSLAVVWFAHRM
                     DLADREGHPVHLLWSALTYWPWLVREILKSALDVSKVILDPQLPASPTVVQFKPRQKT
                     VVGLVTHANSITLTPGTLSVEVGPDRFLVHGLTRESANAAVDSDMDRRVERFERRP"
     gene            568..852
                     /locus_tag="AC16O9_15310"
     CDS             568..852
                     /locus_tag="AC16O9_15310"
                     /inference="COORDINATES: protein motif:HMM:NF015993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrpF/PhaF family protein"
                     /protein_id="HIR6518022.1"
                     /translation="MMFAAAALALLVALALALARAIAGPTVFDRALAANSIGTLAMMI
                     LAVFGFLTGRPEFLDLAILYGLLNVIGTIAVLKFFRYGDLGDPGRPEEER"
     gene            849..1181
                     /gene="mnhG"
                     /locus_tag="AC16O9_15315"
     CDS             849..1181
                     /gene="mnhG"
                     /locus_tag="AC16O9_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HIR6518023.1"
                     /translation="MNVLLEALSWALLAAGGAFCVIGALGLVRLHSFFPRTHAASVID
                     SAGAGLLLAGMLLQAGWTLVAVKLLVIGLLIFFASPTATHALANAARERGVAYDEMTE
                     EGEAPSKP"
     gene            1202..1711
                     /locus_tag="AC16O9_15320"
     CDS             1202..1711
                     /locus_tag="AC16O9_15320"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HIR6518024.1"
                     /translation="MMAATVIAIIAQRNLFSVVVLAGVYSFLMATVLVALDAVDVAMT
                     EASVGAGISTVLLLGALYLCGGEEAKPMARPWLPLAVTLVIVGALVYGTLGLPAFSDP
                     MAPIHTHVVPRYLGQALAETGVPNVVTAVLASYRGYDTLGETVVVFTAGIGVIALLRR
                     ARRGKEKGK"
     gene            1708..2130
                     /locus_tag="AC16O9_15325"
     CDS             1708..2130
                     /locus_tag="AC16O9_15325"
                     /inference="COORDINATES: protein motif:HMM:NF009162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HIR6518025.1"
                     /translation="MRREPVLRVVGKLLIPYMLVFALYVQFHGDFGPGGGFQAGVITA
                     ASIIFYALIFGLPAARRAVPEALVEAMVALGAFLYVAVGFAGLALGGNYLDYFVLHPN
                     PVEGQHRGIFWVEVGVLVTVSGVMLKIFYVFAGRGRES"
     gene            2134..2502
                     /locus_tag="AC16O9_15330"
     CDS             2134..2502
                     /locus_tag="AC16O9_15330"
                     /inference="COORDINATES: protein motif:HMM:NF005621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HIR6518026.1"
                     /translation="MSAFASHYTYWVTVLLMAAGLYIMIARGNLVKKLIGLSIFQTSV
                     YLLYIAPGKLIGGTAPILSQAYKVYSNPLPHVLILTAIVVGIATLALGLAIVVRIREA
                     YGSIEEDEIVAADAKRGDGE"
     gene            2499..3980
                     /locus_tag="AC16O9_15335"
     CDS             2499..3980
                     /locus_tag="AC16O9_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HIR6518027.1"
                     /translation="MISEHLPALQVVVPLAAAPLIVLLRHPGIAWAIAVAASVFAFGA
                     SLGLAAEVARSGVISYAMGSWPPPWGIEYRVDALSAFVLVLVSAIGALVAPYALRSVA
                     AEVPREQRYLYYAVHCLCLAGLLGMTITGDAFNVFVFMEIASLAMYVLIALGRDRRAL
                     LASYQYLILGTIGATFYVIGVGLLYLVTGTLNIADMAERLPQAGAARPVLAALAFITV
                     GLSLKLALFPLHLWLPNAYAYAPSAATALLAATATKVSVYVLLRYYFSVFGAGLVVES
                     LPVPEILIGLSIAAIVTASLVALWQADFKRLLAYSSVAQIGYITLGIGLATRDGLTAA
                     VVHLFNHGITKGALFLLVGGIALRAGGVNFARLQGLARAMPLTCLGVTLAGLSLIGVP
                     GTAGFISKWYLVLGAIEAGHWWLAGLVVLTSLIAVAYVWRFVEAAYLSEPAAGTARIA
                     EAPLPMLAISWVLVGACVWFGFDTSFTVEGARRAALLMLGGTP"
     gene            3977..5428
                     /locus_tag="AC16O9_15340"
     CDS             3977..5428
                     /locus_tag="AC16O9_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR6518028.1"
                     /translation="MTAAQLIAACLVVPAAGAAGIALAGRWPNARETVTLGTAALLFA
                     CVLGLLPTVLAGGIPQLFVLEMLPGIALGFRVEPLGMLFALVASGLWIVNSVYSIGYM
                     RGNEEPHQTRFYVCFALALAATMGIALSANLLTLFVFYEMLTLVTWPLVTHHGDDEAR
                     KGGRVYLGVLLGTSIVLLLPAVIATWAIAGTLEFTPGGILGDKVSGAALGGLLALYMF
                     GIGKAALMPMHRWLPAAMVAPTPVSALLHAVAVVKAGVFAVVKVVVYVFGTGALAATT
                     ASDWLPTVAGFTVIAASVVALRADNLKRRLAYSTVSQLSYVVLAAAILAPLSVIGATL
                     HIAAHAVGKITLFFAAGAIYTAAHKTEVSQLDGIGRRMPWTMGAFAVGVLSMIGLPPA
                     AGFVSKWYMLAGALQAEHWVAVGVIAASTLLNAAYFVPIVYRAFFRAPHGERHGEAPL
                     PMVLALVATALATLALFFFPQVPLALARALPGG"
     gene            5430..5687
                     /locus_tag="AC16O9_15345"
     CDS             5430..5687
                     /locus_tag="AC16O9_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518029.1"
                     /translation="MSDHWLVRPSTIRLLWRVFIAVLALTVIAEFLVVHHPHFAVESV
                     PAFGAWYGFVACAGMILFAKGLALFLKRPDTYYEGEEGRDA"
     gene            5680..7365
                     /locus_tag="AC16O9_15350"
     CDS             5680..7365
                     /locus_tag="AC16O9_15350"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HIR6518030.1"
                     /translation="MPSAWLHPGLLVIVAGLALALLRGRAREAVALVAPLAALWLVLA
                     APTGIAWSGTWLGLEVRPLAYDALSRLFAAIFAIMAFAGGLYALNQKSRLELPAAYVY
                     AGSALGAVLAGDLVTLFVFWELMAVGSTLVIWSAGTGPSYRASMRYLSIHLLGGVLLF
                     AGIAGHVAATGSTEFTRLALDAPAHWLILAGFLINAGAPPLSAWLPDAYPEASWSGMV
                     FLSAFTTKTAVYVLLRGFPGTELLVWVGLFMVFYGIVYALLENDMRRILAYSIVNQVG
                     FMVTGIGIGTEMALNGAAAHAFTHIIYKALPLMSAGSVLYATQKRKCTDLGGLFRTMP
                     LTTVCGTIGALAISSFPLTSGFVSKSMISQAAGDEHLLWVWLALAAATAGVFLHAGIK
                     FPWFVFFQKDSGLRPPEPPLNMRLAMLLFAALCIGLGVFYQPLYALLPFSTGYVPYTG
                     SHVVVQLELLLFSGLAFFVMLPMLRRTLTITLDVDWFYRRLAPALAAALLAALRAVGA
                     VLGAARDGGMRRIKTGAHALHGPQALFARTWTTGGMALWAALLLAGYLLVVYL"
     gene            7502..8635
                     /locus_tag="AC16O9_15355"
     CDS             7502..8635
                     /locus_tag="AC16O9_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6518031.1"
                     /translation="MNKEQHKPETARRKFLTGAAAATAGAATLGFPMIAKSQSPIVIK
                     MQGSWSATDIFNEMAQEFVTRVNSMSGGRLRVDYLVGGAVVKPFEVMDAVSKGVLDAG
                     HTVPVYWYGKSKVASLFGSGPINGSDAFQTLAWIYRGGGLELYKELLDKLGLNVVGFF
                     AMPMPTQPLGWFKKPITDAKQLKGLKYRTVGLAADLFQEMGAKVTQLPGGEIIPALER
                     GVIDAFEFNNPTSDSRFGAQDVIKNYEMGSFHQAMEFFEIIINKKKWDSMGKDLQAIW
                     QYGVEAASQANYWTGMDNYSRDLQVLIDKHKVKVIRTPKSVFQAQLNAWDVLTKRLSA
                     EDPFFAKVVESQLAWAKRVGYYWFLNDAEFKMGYEHVFKTKLA"
     gene            8731..9327
                     /locus_tag="AC16O9_15360"
     CDS             8731..9327
                     /locus_tag="AC16O9_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6518032.1"
                     /translation="MISRFIYGIDQLSKTVGHAFAWCIIILTFGTSYEVFVRYALNNP
                     TSWAFDFSYIMYGALFFMAGAYALSRNGHVRGDFIYRMWRPRMQAGVELTLYVLFYFP
                     GVLALVYAGWLYGFDSFKIREVSVNSPAGIPVWQLKILIPFGAALLALQGVAETLRCI
                     LCLRDGQWPPRLHDVEELEDVLAQQHAAEAAAAKEGAK"
     gene            9324..>10150
                     /locus_tag="AC16O9_15365"
     CDS             9324..>10150
                     /locus_tag="AC16O9_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR6518033.1"
                     /translation="MSDPVIALVMLGLFIFVILLGFPIAFTLMGMAVGFGFYAYYRPD
                     QEFFDSRVFYLLTQNTWSIMSNDVLVAVPLFLFMGYVVERSNILDKLFYSLQVALRHI
                     PGSMAVAALATCAMFATATGIVGAVVTLMGLLAFPVMLRAGYDQRLSAGVICAGGTLG
                     ILIPPSIMLIVYSATASVSVVRLYAAALLPGFLLAGLYVGYVITRAVVNPKLCPPLPK
                     EESEVPWAKAGLLLLQAFVPLAVLIFSVLGSILFGLATPTEAAAEYTISMIEGGIRIP
                     "
BASE COUNT         1320 a         3480 c         3471 g         1879 t
ORIGIN      
        1 cgctcgcgca cacggtgcgc gaatccacgg cgcgctgggc gggcgacaag ccgcgcgcca
       61 gcggctagaa tcgcgcgctg cggcgcacgc acccatgctc cacttcgcga tacttttcgt
      121 cctgttctac gcgttctgga tcctgatgtc gggattcttc acgccgttcc tgctcgcggc
      181 gggcgcggcg accagcctgg ccgtggtgtg gttcgcgcac cgcatggacc tggcggaccg
      241 cgaaggccat cccgtgcacc tcctgtggtc ggcgctcacg tactggccgt ggctcgtgcg
      301 cgagatcctg aaatccgcgc tcgacgtctc gaaggtgatt ctcgacccgc agctgccggc
      361 ctcgccgacg gtggtgcagt tcaagccgcg gcagaagacc gtggtcggcc tggtgaccca
      421 cgccaactcc atcaccctga ccccgggcac gctgtccgtc gaggtggggc ccgatcgctt
      481 cctggtgcac gggctcacgc gcgagagcgc caacgcggcg gtcgacagcg acatggaccg
      541 gcgcgtcgag cgcttcgagc gcaggccatg atgttcgccg ccgcggcgct cgcgctgctc
      601 gtcgcgctcg ccctggcgct cgcgcgcgcg atcgcgggcc cgacggtgtt cgaccgcgcg
      661 cttgccgcca attccatcgg cacgctcgcc atgatgatcc tggcggtgtt cggtttcctc
      721 accgggcgcc ccgagttcct cgatctcgcc atcctctacg gactgctcaa cgtcatcggc
      781 acgatcgccg tgctcaagtt cttccgctac ggcgatctcg gcgacccggg caggccggag
      841 gaagagcggt gaacgtgctg ctcgaagccc tgtcctgggc gctgctcgcc gccggcggcg
      901 cgttctgcgt catcggcgcg ctcggcttgg tgcgcctgca cagcttcttt ccgcgcaccc
      961 acgccgcgag cgtcatcgac tcggcgggcg ccgggctgct gctcgccggc atgctgctgc
     1021 aggcgggctg gacgctggtg gcggtgaagc tcctggtgat cgggctgctc atcttctttg
     1081 cttcgcccac cgcgacgcac gcgcttgcca acgccgcgcg cgagcgcggg gtggcatacg
     1141 acgagatgac cgaggagggg gaggcgccat cgaagccctg atcatccagg cgctgctcac
     1201 gatgatggcg gcgacggtca tcgccatcat cgcgcagagg aacctgttct cggtggtggt
     1261 gctcgccggc gtctacagct tcctcatggc gacggtgctg gtggcgctcg atgcggtgga
     1321 cgtcgccatg accgaggcgt cggtgggcgc cggcatctcg acggtgctgc tgctcggcgc
     1381 gctgtatttg tgcggcggcg aggaggccaa gccgatggcg cggccgtggc tgccgctcgc
     1441 ggtgacgctg gtgatcgtcg gcgcgctggt gtacggcacc ctcggcctgc cggctttctc
     1501 cgatcccatg gcgccgatcc atacgcacgt cgtgccgcgc tacctgggcc aggcgctcgc
     1561 cgaaacgggc gttcccaacg tggtgaccgc cgtgctcgcg agctaccgcg gctacgacac
     1621 gctcggcgaa acggtggtgg tgttcacggc gggcataggc gtgatcgcgc tgctgcgccg
     1681 cgcgcgccgc ggcaaggaga agggcaagtg aggcgcgagc ccgtcctgcg cgtggtgggc
     1741 aagctgctca ttccgtacat gctggtgttc gccctgtacg tgcagttcca tggcgatttc
     1801 ggcccgggcg gcggcttcca ggcgggagtg atcacggcgg cgtcgatcat cttctacgcg
     1861 ctcatcttcg gtctgccggc cgcgcggcgc gcagtacccg aggccctggt cgaagccatg
     1921 gtggcgctcg gcgcgttcct gtacgtggcg gtcggctttg ccgggctcgc gctcggcggc
     1981 aactacctgg attacttcgt gctccacccc aacccggtag aaggccagca tcgcggcatc
     2041 ttctgggtcg aggtcggcgt gctggtcacg gtgtcgggcg tgatgctgaa gatcttctac
     2101 gtgttcgccg ggcgcggccg cgagagctga agtatgtcgg cgttcgcgag tcactatacc
     2161 tactgggtca ccgtgctgct gatggccgcg ggcctgtaca tcatgatcgc gcgcggcaac
     2221 ctggtgaaga agctcatcgg gctgtcgatc ttccagacct cggtgtacct gctgtacatc
     2281 gcgcccggca agctgatcgg cggcaccgcg cccatcctgt cccaggccta caaggtgtat
     2341 tcgaacccgc tgccgcacgt gctgatcctc accgcgatcg tggtggggat cgcgacgctc
     2401 gcgctcggcc tcgccatcgt ggtgcgcatc cgcgaggcct acggcagcat cgaggaagac
     2461 gagatcgtcg ccgcggacgc gaagcgcggg gacggcgagt gatctccgag cacctgccgg
     2521 cgctgcaggt ggtcgtgccg ctcgccgcgg cgccgctcat cgtgctgctg cgccatccgg
     2581 gcatcgcctg ggcgatcgcc gtggcggcga gcgtgttcgc gttcggcgcg agcctcgggc
     2641 tcgctgcgga ggtggcgcgc agcggcgtca tctcctacgc catgggcagc tggccgccgc
     2701 cctggggcat cgagtaccgc gtcgacgcgc tgtcggcgtt cgtgctggta ctggtatcgg
     2761 cgatcggcgc gctggtcgcg ccctacgccc tgcgcagcgt ggccgcggag gtaccgcgcg
     2821 agcagcgcta cctgtattac gcggtgcact gcctgtgcct cgcgggcctg ctcggcatga
     2881 cgatcaccgg cgacgccttc aacgtgttcg tgttcatgga gatcgcctcg ctcgcgatgt
     2941 acgtgctgat cgcgctgggg cgcgaccggc gcgcgctgct cgcctcgtac cagtacctga
     3001 tcctcggcac catcggcgcc acgttctacg tgattggcgt cgggctgctc tacctcgtca
     3061 ccggcacgct caacatcgcc gacatggccg agcgcctgcc gcaggccggc gcggcgcggc
     3121 cggtgctggc cgcgctcgcg ttcatcaccg tcgggctgtc gctcaagctc gcgctgttcc
     3181 ccttgcacct ctggctcccg aacgcctatg cctacgcgcc ctccgcggcc accgcgctcc
     3241 tcgccgcgac cgccaccaag gtctcggtgt acgtcctgct gcgctattac ttttcggtgt
     3301 tcggcgccgg gctggtggtc gagtccctgc cggtgccgga gattttgatc ggcctgtcga
     3361 tcgccgccat cgtcaccgcg tcgctcgtcg cgctgtggca ggcggacttc aagcgcctgc
     3421 tcgcctactc gagcgtggcg cagatcgggt acatcacgct cggcatcggt cttgcgacgc
     3481 gcgacgggct gaccgcggcg gtggtgcacc tgttcaacca cggcattacc aaaggggccc
     3541 tgttcctgct cgtcggcggc atcgcgctgc gcgcgggtgg cgtgaatttc gcgcgcctgc
     3601 agggcctcgc gcgcgcgatg ccgctcacct gcctcggcgt gacgcttgcc gggctgtcgc
     3661 tcatcggcgt gccgggcacg gccgggttca tcagcaagtg gtatctcgtg ctcggcgcca
     3721 ttgaggccgg gcactggtgg ctcgcggggc tggtggtact cacctcgctg atcgccgtgg
     3781 cctacgtctg gcgcttcgtc gaggccgctt acctcagcga gccggccgcg ggcacggcgc
     3841 gcatcgcgga ggcgccgctg ccgatgctgg cgatttcctg ggtgctggtc ggtgcctgcg
     3901 tctggttcgg cttcgacacg tcctttacgg tcgagggcgc gcgccgcgcg gcactgctca
     3961 tgctgggcgg cacgccgtga ccgccgcgca gctgatcgcc gcgtgcctcg tcgtgcccgc
     4021 cgcgggcgcg gcgggcatcg cgctcgccgg acgctggccg aacgcgcgcg agacggtgac
     4081 gctcggcacc gcggcgctgc tgttcgcgtg cgtgctcggc ctgctgccta ccgtgctggc
     4141 gggcggcatt ccgcagctgt tcgtgctcga gatgctcccc ggcatcgcgc tcggctttcg
     4201 cgtcgagccg ctcgggatgc tcttcgcgct ggtggcctcc gggctgtgga tcgtgaactc
     4261 ggtctactcc atcggctaca tgcgcggcaa cgaggagccg caccagacgc gcttttacgt
     4321 ctgcttcgcg ctggcgctcg ccgccaccat gggcatcgcc ctgtcggcaa acctgctgac
     4381 gctgttcgtg ttctacgaga tgctgacgct ggtcacctgg ccgctggtga cgcaccacgg
     4441 cgacgacgag gcgagaaagg gcgggcgcgt gtacctcggc gtgctgctcg gcacgtcgat
     4501 cgtgctgctg ctgccggccg tgatcgccac ctgggcgatt gccggaacgc tggagttcac
     4561 cccgggcggc attctcggcg acaaggtgtc gggcgccgcg ctcggggggc tgctggcgct
     4621 gtacatgttc ggcattggca aggcggcgct gatgccgatg caccgctggc tgccggcggc
     4681 gatggtggcg ccgacgccgg tgtcggcgct gctgcacgcg gtggcggtgg tgaaggcggg
     4741 cgtgttcgcg gtggtgaaag tcgtcgtcta cgtcttcggc accggggcgc tcgccgccac
     4801 cacggcttcc gactggctgc cgacggtcgc cggcttcacc gtcatcgccg cctccgtggt
     4861 cgcgcttcgc gccgacaatc tgaagcgccg cctcgcctac tcgacggtga gccagctgtc
     4921 ctacgtggtg ctggcggcgg cgatcctcgc gcccctttcg gtgatcggag cgaccctgca
     4981 tatcgccgcg cacgccgtcg gcaagatcac cctgttcttc gctgccggcg cgatctacac
     5041 cgctgcccac aagaccgaag tcagccagct cgacggcatc ggccggcgca tgccctggac
     5101 catgggcgca tttgccgtcg gggtgctgtc gatgatcggg ctgccgccgg ccgccggctt
     5161 cgtcagcaag tggtacatgc tggcgggcgc actccaggcc gagcactggg tggcggtcgg
     5221 cgtgatcgcc gcgagcacgc tgctcaacgc cgcctacttc gtgccgatcg tgtaccgcgc
     5281 gttctttcgc gcgccgcacg gcgagcgcca cggcgaggcg ccgctgccga tggtgctcgc
     5341 gctcgtcgcc accgcgctcg ccacgctggc gttgttcttc ttcccgcaag tgccgctcgc
     5401 gctggcgcgc gcgctgcccg gaggctgaga tgagcgatca ctggctggta cgtccctcca
     5461 cgatccggct gctgtggcgg gtgttcatcg ccgtgctcgc ccttaccgtg atcgcggagt
     5521 tcctcgtcgt gcaccacccg cactttgccg tcgagtcggt gcccgcgttc ggcgcctggt
     5581 acggctttgt cgcctgtgcc ggcatgattc tgttcgccaa ggggctggcg ctgttcctca
     5641 agcggcccga cacctactac gagggcgagg agggccgcga tgcctagcgc ctggctgcat
     5701 ccgggcctgc tggtgatcgt cgcgggcctc gcgctcgcgc tgctgcgcgg ccgggcgcgc
     5761 gaggcggtgg cgctcgtcgc cccgctcgcc gcgttgtggc tcgtgcttgc cgcgcccacg
     5821 ggcatcgcct ggagcggcac ctggctcggc ctggaggtgc ggccgctcgc ctacgatgcg
     5881 ctgtcgcggc tgttcgccgc gatcttcgcg atcatggcgt tcgccggcgg cctgtatgcc
     5941 ctcaaccaga agagccggct cgagctgccc gcggcctacg tctacgccgg atcggcgctt
     6001 ggcgcggtgc tcgccggcga cctggtcacg ctgttcgtgt tctgggaact gatggcggtc
     6061 ggctcgacgc tcgtcatctg gagcgcgggg accggaccgt cgtaccgcgc gagcatgcgc
     6121 tacctttcga ttcacctgct gggcggcgtg ctgctgttcg ccggcatcgc gggccacgtt
     6181 gccgcgaccg gatccacgga attcacgcgc ctggcgctcg acgcgcccgc ccactggctg
     6241 atcctcgccg ggttcctgat caacgccggt gcgccgccgc tttcggcgtg gctgcccgac
     6301 gcctacccgg aagcgagctg gagcggcatg gtgttcctgt cggcgttcac caccaagacc
     6361 gcggtgtacg tgctgctgcg cggcttcccg ggcaccgagc tcctcgtgtg ggtgggcctg
     6421 ttcatggtgt tctacggcat cgtctacgcg ctgctcgaga acgacatgcg gcgcatcctc
     6481 gcctacagca tcgtcaacca ggtcggcttc atggtgaccg gcatcggcat cggcacggag
     6541 atggcgctga acggcgccgc ggcgcacgcc ttcacgcaca tcatctacaa ggccctgccg
     6601 ctcatgtccg cggggtcggt gctctacgct acgcaaaagc gcaagtgcac cgacctcggg
     6661 ggcctgttcc gcaccatgcc gctcacgacg gtgtgcggca ccatcggcgc gctcgcgatc
     6721 tcgtcgttcc cgctcacctc gggcttcgtg tcgaagtcga tgatctcgca ggccgcgggc
     6781 gatgagcacc tgctgtgggt gtggctcgcg ctcgccgcgg cgaccgcggg tgtcttcctg
     6841 cacgcaggga tcaagttccc gtggttcgtg ttcttccaga aggattcggg cctgcgcccg
     6901 cccgagccgc cgctcaacat gcgcctcgcg atgctgctgt ttgcggcgct gtgcatcggc
     6961 ctcggggtct tctaccagcc gctgtatgcc ctgctgccct tcagcacggg ctacgtgccc
     7021 tacaccgggt cccacgtcgt cgtgcagctc gagttgctgc tgttctcggg gctcgcgttc
     7081 tttgtcatgc tgcccatgct gcggcgcaca ctcaccatca cgctggacgt cgactggttc
     7141 taccgccggc ttgcgccggc gctcgccgcc gccctcctgg ctgctctgcg cgcggtcggg
     7201 gcggtgctcg gggcggcgcg ggacggcggc atgcgacgaa tcaagaccgg cgcgcatgcg
     7261 ttgcacgggc cacaggcgct gttcgcgcgc acctggacga cgggcggaat ggcgctgtgg
     7321 gccgcgctat tgctggccgg gtatctcctg gtcgtctacc tctgagcgcg cgagccgcat
     7381 ccgcgtttca catcgtgaag tcgccttgcg gcggctgaag ggggggcgct agaatggccg
     7441 agtactacac acacggggcc caagccccgc gcagcaccca caccgaaaga ggagacccag
     7501 catgaacaag gaacaacata agcccgaaac tgcgcgccgc aagttcctga ccggcgcggc
     7561 ggcggcgacg gcgggcgcgg cgacgctcgg cttcccgatg atcgccaagt cgcaatcgcc
     7621 gatcgtcatc aagatgcagg gatcgtggag cgccacggac atcttcaacg agatggcgca
     7681 ggagttcgtg acgcgcgtca actcgatgtc gggcggccgg ctgcgcgtcg actacctggt
     7741 cggcggcgcg gtcgtgaagc ccttcgaggt gatggacgcg gtgtccaagg gcgtgctgga
     7801 cgccggccac acggtgccgg tgtactggta cgggaagtcc aaggtggcgt cgctgttcgg
     7861 ctccgggccg atcaacggct ccgacgcgtt ccagacgctc gcctggatct accgcggcgg
     7921 cggcctcgag ctgtacaagg aactgctcga caagctcggc ctcaacgtcg tcggcttctt
     7981 cgccatgccg atgcccaccc agcccctggg ctggttcaag aagcccatca cggacgccaa
     8041 gcagctcaag ggcctgaaat accgcactgt gggcctggcg gcggacctgt tccaggagat
     8101 gggcgccaag gtgacccagc tgccgggcgg cgaaatcatt cccgccctcg agcgcggcgt
     8161 gatcgacgcc ttcgagttca acaacccgac ctcggacagc cgcttcggcg cccaggacgt
     8221 gatcaagaac tacgagatgg gcagcttcca ccaggcgatg gagttcttcg agatcatcat
     8281 caacaagaag aagtgggact cgatgggcaa ggacctgcag gccatctggc agtacggcgt
     8341 cgaggccgcg tcccaagcca actactggac cggcatggac aactactcca gggaccttca
     8401 ggttctcatc gacaagcaca aggtcaaagt gatccgcacg ccgaagtcgg tgttccaggc
     8461 gcagctcaac gcctgggacg tgctgacgaa gcggctctcg gccgaggatc cgttcttcgc
     8521 caaggtggtc gagtcgcagc tcgcctgggc caagcgcgtg gggtactact ggttcctcaa
     8581 cgacgccgag ttcaagatgg gctacgagca cgtgttcaag accaagctgg cgtagcctta
     8641 gaggcgcaca ggcaggatcc agccggggcg acgcttcgcg cgtcgccccg ttttcttcgg
     8701 ggcgcgtagt cgcgcgcggg ggctcgcaaa atgatcagcc gcttcatcta cggcatcgac
     8761 cagctgagca agacagtcgg ccatgctttt gcgtggtgca tcatcatcct cacgtttggc
     8821 acctcctacg aggtgttcgt gcgctacgcg ctgaacaacc cgacgagctg ggcgttcgat
     8881 ttcagctaca tcatgtacgg cgccctgttc ttcatggcgg gcgcgtacgc gctgtcgcgc
     8941 aacggccacg tgcgcggcga cttcatctac cgcatgtggc gcccgcgtat gcaggccgga
     9001 gtcgagctga cgctgtacgt gctgttctat tttccgggcg tgctggcgct ggtgtacgcc
     9061 gggtggctgt atgggttcga ttccttcaag atccgcgagg tgagcgtcaa cagcccggcc
     9121 ggcatcccgg tgtggcagct gaagatcctg attccgttcg gcgccgcgct gctcgcgctg
     9181 cagggcgtcg ccgagacgct gcgctgcatc ctgtgcctgc gcgacggcca gtggccgccg
     9241 cgcctgcacg acgtcgagga gctcgaggac gtgctcgcgc agcagcacgc cgccgaggcg
     9301 gccgcagcca aggagggcgc caagtgagcg atccggtcat cgccctggtg atgctggggc
     9361 tgttcatttt cgtcatcctg ctcggctttc cgatcgcctt cacgctgatg ggcatggcgg
     9421 tgggcttcgg cttctacgcc tactaccgcc cggaccagga gttcttcgac agccgcgtct
     9481 tctacctgct gacgcagaac acctggagca tcatgagcaa cgacgtgctc gttgcggtgc
     9541 ccctgttcct gttcatgggc tacgtggtcg aacgctcgaa catcctcgac aagctgttct
     9601 acagcctgca ggtcgcgctg cgccacatcc cgggctcgat ggcggtggcg gcgctcgcca
     9661 cctgcgccat gttcgccacc gccaccggca tcgtcggtgc ggtggtgacg ctgatggggc
     9721 tgctcgcgtt cccggtgatg ctgcgcgccg gctacgacca gcggctctcc gcgggggtga
     9781 tctgcgccgg cggcacgctc ggcatcctca tcccgccctc gatcatgctg atcgtgtact
     9841 cggccactgc ctcggtctcc gtggtgcgcc tgtatgccgc ggcgctgctg ccggggttcc
     9901 tcctcgccgg gctgtacgtc ggctacgtga tcacgcgcgc ggtggtcaat ccgaagctct
     9961 gcccgccgct gccgaaggag gaaagcgagg tgccctgggc caaggcgggc ctgctgctcc
    10021 tgcaggcgtt cgtgccgctc gccgtgctga tcttctcggt gctcggctcg atcctgttcg
    10081 gcctggcgac gccgaccgag gcggcggccg aatacacgat cagcatgatc gagggcggga
    10141 tcaggatacc
//
LOCUS       DBNHKE010000227        37936 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_149156, whole genome shotgun sequence.
ACCESSION   DBNHKE010000227 DBNHKE010000000
VERSION     DBNHKE010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 37936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 37936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37936
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_149156"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16O9_15370"
     CDS             complement(<1..534)
                     /locus_tag="AC16O9_15370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6518034.1"
                     /translation="MTLTLFDLDNTLLAGDSDYEWGQFLIARGVLSRAEYEAQNARFF
                     EQYKAGTLDIHEYLGFALGPLAAHTPEDLARWHEDFMRERVRPMLLPRALELVARHLA
                     AGELCAVVTATNSFVTAPIARELGVPHLVATEPERIAGRFTGRLAGTPCFREGKLRRV
                     DEWLAALGRRFEDFEDSR"
     gene            complement(531..1136)
                     /locus_tag="AC16O9_15375"
     CDS             complement(531..1136)
                     /locus_tag="AC16O9_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdaA/DnaA family protein"
                     /protein_id="HIR6518035.1"
                     /translation="MTRQLALPLSAPPVPTLENFVPGANGELLERLRELAAGRLREAV
                     IYLWGAAGCGRSHLLAASARPGVTLADDVEALDAAGQQGLFNAINRAREDGGTVLAAG
                     AAPPAQLALREDLRSRLGWGLVYEVKPLTDAERAVYLRAEAARRGLRLADEVVWYLLT
                     HVKRDMPSLRALLEQLDRASLEQRRPVTLPLVREALKAFEQ"
     gene            complement(1133..2197)
                     /locus_tag="AC16O9_15380"
     CDS             complement(1133..2197)
                     /locus_tag="AC16O9_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6518036.1"
                     /translation="MPLSANPHLWGWLAVALVVGGLLYLLSPILAPFLFAAILAYILD
                     PLVERLTGKFVPRTLAVVLVMSGVLALLVALALVVLPLFAKELRLLAERLPAFIAWLN
                     QKLVPFAERYFGGALQLDVASVRALATEVVAENQDLVGKLLGSLRIGGLALLAFLVNL
                     LLVPVVLFYLLRDWHQMLARVDRLIPRHLHQRTRTILREVDSVLAEFLRGQLAVILVM
                     SVYFVLALWLAGLEFALPIGLITGLLVFVPYVGALLGFALGTVTALMQFDSLWGLAWV
                     WLAFGVGQTLEGMVVTPWLVGERIGLHPVAVIFALLAFGQVFGFFGVLMALPASAALL
                     VALRHVREGYLASSLYGGGK"
     gene            2241..3248
                     /gene="purM"
                     /locus_tag="AC16O9_15385"
     CDS             2241..3248
                     /gene="purM"
                     /locus_tag="AC16O9_15385"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR6518037.1"
                     /translation="MADPLSYRDAGVDIDAGDALVEAIKPFARRTMRPEVMAGIGGFG
                     ALCRIPRKYRQPVLVSGTDGVGTKLKLAFAFGRHDTVGIDLVAMSVNDVLVQGAEPLF
                     FLDYFACGKLEPRVAAMVVKGIARGCELAGCALIGGETAEMPGMYPAGEYDLAGFCVG
                     VVEKRRIISGRSIRPGDVLLGLASSGAHSNGYSLIRRILEREAPDGALTDALMQPTRI
                     YVRSILQLLRTVNVKGLAHITGGGLVGNVPRALPPRTRAVIDARAWPRPALFQWLQQK
                     GAVDEAEMHRVFNCGIGMVAVVAASDARRATKRLRALGETVYEIGLIERARGEAEAVV
                     S"
     gene            complement(3241..3705)
                     /locus_tag="AC16O9_15390"
     CDS             complement(3241..3705)
                     /locus_tag="AC16O9_15390"
                     /inference="COORDINATES: protein motif:HMM:NF015126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HIR6518038.1"
                     /translation="MATRKKAKAKPKRRKALAKRPAARRKVVKRARKPARKAPVRRAA
                     PKAARRKIVKRRAPEPRPAAAPEPAAPGERIGVVTHFYGEPSVAIVKLETGMLRVGDT
                     IHIRGHTTDFSQRVASLQVEHAPVNEVGPEDDFGVRVDQHVREHDVVYKVTS"
     gene            complement(3734..4600)
                     /locus_tag="AC16O9_15395"
     CDS             complement(3734..4600)
                     /locus_tag="AC16O9_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6518039.1"
                     /translation="MRAPALLALLCGATFIALSPIWVRLADVGPTASAFWRVALAVPL
                     LWAAYALAPGARGARRADWRMLGVAGVAFAGDLAFWHWSIQFTSVANSTLLANLASIF
                     VTLAAWLLWRQKPRALFLVALGLALVGVGMLVRTSVDFAPRALLGDALGVVTAMFYAW
                     YLLTVKGLRDRGAATLRLMAVTSTVTALVLLPAALASGEVMLPAGAGGWLVLLGLAWI
                     THAGGQGLIAYALAHLPAAFSSVGLLFQPVMAAAFAWVLLGEPLVALQVAGGVVVLAG
                     IYLARRSSLGTE"
     gene            4630..8067
                     /gene="dnaE"
                     /locus_tag="AC16O9_15400"
     CDS             4630..8067
                     /gene="dnaE"
                     /locus_tag="AC16O9_15400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6518040.1"
                     /translation="MAAPRFVHLRLHSEYSVTDGIVRLDAAVGRAARDGMPALALTDS
                     ANVFGMVKFYQAARAAGVKPIVGADCWVQGAAEADKPARVLLLCASRTGYLRLCELLS
                     RAWLQNQRQGRAEIARAWLAESDNEGLIALSGAGAGDVGAALAAGNAAAAERLAREWS
                     ARFPGRFYLELQRAGFAHGETLVARSVELAARLGLPVVATHPVQFLDPEDFKAHEARV
                     CIAQGYVLGDQRRPRQFTPEQYLKSQDEMARLFADLPQALDNALEIARRCNLQIELGR
                     SRLPAFPTPPGVTLDQYLRRQAEQGLATRLAKLFADAAERARAAPHYQERLDFEIRTI
                     IQMGFAGYFLIVADFINWAKQNGVPVGPGRGSGAGSLVAYSLGITDLDPITYNLLFER
                     FLNPERVSMPDFDIDFCQDGRDRVIDYVRQKYGADSVSQIATFGTMAARAVVRDCGRV
                     LDLGYNFCDQVAKLIPVQPGRTITLEDARSMEPLLAQREKKEDEVRELLALGEKLEGL
                     TRNVGMHAGGVLIAPGKLTDFCPLYAAEGGVNVISQLDKDDVEAVGLVKFDFLGLTTL
                     TILDWAERYVRQMGDDQFALDRIPLDDAATYQLLSAGNTTAVFQLESRGMRDLIKRAR
                     PDRFEDVIALVALYRPGPMDLIPDFIARKHGSQRVEYLDPRLEPILRPTYGIMVYQEQ
                     VMQIGQVIGGLTLGGADLLRRAMGKKKAEEMAQQRDVFVAGAEKNGLSRTKATQLFDL
                     MEKFAGYGFNRSHAAAYALLAYQTAYMKAHHASAFMAANLSLVMGDTDKVRQFHDDAL
                     ANGLTIQAPDINASEFRFVPVDEKTLRYGLGAVRGTGESAIHAIVEARKAGPFADLFD
                     LCRRVDKRAVNRRVIEALVRAGALDALEPNRASLLATAGRALEAAEQRERQAAQVSLF
                     GDAADADGAERALVPAQPWGLKQKLTEEKAALGFALSGHLFSVYEHDLAGFPRVPLAQ
                     LAAAEHRVWLAGVVASARTQMTRRGRMMVVMLDDGSAQLELSVFNELFERHRDKLKED
                     ALLIVQGKAQRDEFSGGLRVTADELYDLGALRERFAARLRLEMNGHSDARQLMELLTP
                     YRAQGACPVFVHYETGAAACDVALGDAWRVRPDGQLLERLGAWLAPENVQVVYAGGV"
     gene            complement(8064..9026)
                     /locus_tag="AC16O9_15405"
     CDS             complement(8064..9026)
                     /locus_tag="AC16O9_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518041.1"
                     /translation="MLATFPMSLGTLSAPRVYSASDLCRAMLAEGAYARPLEAACLDR
                     TLRHDAARGLLEVQAGASWRSLRPYVGADFLPGSVGESVAANRAGPDGEPVVRHLHAF
                     TLATGAGELRRVSRERAADLFRLAVGGFGAFGPFYSLTLDVASLARSAARSAAPVRIE
                     LADEPPGGQSTALELLVPPQRSDEVVDRTRAALAERRCCLRLLEARCVHPEAETFLRW
                     ARSEYVALHIEFDTRATLGACASAAQLRTHLIDLALAAGGGFMPGSLPLATRAQAEAC
                     YPMLGAFLAEKRRLDPAERVLSAWYRGTRRVWRAESCRVRWAKD"
     gene            9142..9921
                     /locus_tag="AC16O9_15410"
     CDS             9142..9921
                     /locus_tag="AC16O9_15410"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6518042.1"
                     /translation="MPLTHRIVDALKRSLRARGMTYAALARELRLSEASVKRLFSRHT
                     FTLSRMEDVLRVLDLDLAELARMTRGTQGQDIELSLEQETLLASDERLLSVFWLLLNG
                     WRFDDIVEAFVITRTELTLALAKFERARLIDWDAGERVRLRVAKDFVWRAGGPVKKAY
                     GLRVTAEFLRSRFSGPLEMLRFEARDLSPASAAMLRRRLERVAAEFNELAEVDAGLPA
                     RRREGVGLLIACRPWAFSAMNALKRRDTGRDQAVGQRSNRA"
     gene            complement(9888..10178)
                     /locus_tag="AC16O9_15415"
     CDS             complement(9888..10178)
                     /locus_tag="AC16O9_15415"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR6518043.1"
                     /translation="MQVRTIRLCIRGRVQGVGFRDAMSREAERLGLTGWVRNRAGGDV
                     EAVAQGDAAALEALTAWARRGPTAARVEGVDCGPPAPEHARSYARFERWPTA"
     gene            10298..10693
                     /locus_tag="AC16O9_15420"
     CDS             10298..10693
                     /locus_tag="AC16O9_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518044.1"
                     /translation="MRAALIVVAILALAAFLAVAVVLPQMAGSEAREAAQALIAGTEG
                     AQKQIAAAAQSAGSLTGTGKDLKMPARSDPKHGELKWIIETNGAIRGWNEKNAIEISI
                     TPILQGGTVTWNCRGYPITAMPANCGGKS"
     gene            complement(10718..11944)
                     /locus_tag="AC16O9_15425"
     CDS             complement(10718..11944)
                     /locus_tag="AC16O9_15425"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6518045.1"
                     /translation="MGAMDDQKLGRFEVLAEIGRGAMGIVYKAKDPMLERTVAIKTIN
                     MSMDRDGAEMYEKRFNQEARAAGGLNHPNIVTVYDIGKTDTECYMAMEYIEGAELRTL
                     LLPGKPMPVPRALSIAAQVAEGLAYAHERGVVHRDIKPANIMVPETGAVKITDFGIAR
                     MRSSNVQTQTGMMMGSPKYMSPEQVIGKRADHRTDIFSLGVILYEMLTGSTPFSGESV
                     NAVMYQIVNFVPPAPSAINPAAPAVLDGIVAGMLAKSLEERFQSAAEVARGLRECERQ
                     VHGVAATSATSTLPLGSLAEGATPTVVDKDVKSAILAQTLERSRRAEDGAPAPDAAPA
                     RGLSRTFDSLEATQRLVALSDAALTRDPAANHPLQLSMQTTSTALSGGWRLRDTLVVA
                     AGAISGLIAAAIIVLL"
     gene            complement(11954..12286)
                     /locus_tag="AC16O9_15430"
     CDS             complement(11954..12286)
                     /locus_tag="AC16O9_15430"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HIR6518046.1"
                     /translation="MRALQEDFRSLFGSAKGGPALAVGIRQYEEGKYAEAAESLNTSL
                     YQGLSSPDRVKAHKYLAFIDCVSGRPAACRREFDRALAIDPGMELSAAEVGHPIWGPV
                     FRAVKAGR"
     gene            complement(12435..13643)
                     /locus_tag="AC16O9_15435"
     CDS             complement(12435..13643)
                     /locus_tag="AC16O9_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6518047.1"
                     /translation="MIEQPSRTLVCSIVFLDIVEYSAKPVAEQLLLKQQFNKLLSSAL
                     ENVAPRDRVVLDTGDGAGITFLGDPEDALFVAMSLRAAARAESSGAIAVRIGINLGPV
                     RLVKDINGQINIIGDGINVAQRVMTFAQAGQLLVSRSFYEVVSCLSLDYASLFSYVGA
                     RTDKHVREHEVYSVGIGTDAARRVIETSSRRVAPRRRRGPRLIGELTDVRPFGVHRLA
                     FLGAPLAFVLLVSGGVAVKAQISHPPLAIKPGPAALQMAAPEPARAEAPANGEKPAAA
                     ESAQPQPPVTPAPVAAAPTQRGQLRETLAARTPRPAREARKPEAAPAAAPAQIAASAA
                     AGTIQLAILPWGEVFVDGKSRGVSPPLRSLEVPLGTHTVEVRNTSFPTHTRRVEVRSG
                     EPVRIRHRFR"
     gene            complement(13681..15747)
                     /gene="metG"
                     /locus_tag="AC16O9_15440"
     CDS             complement(13681..15747)
                     /gene="metG"
                     /locus_tag="AC16O9_15440"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6518048.1"
                     /translation="MSRRRLFVTTALPYANGAFHIGHIMEYIQADIWVRFQRMRGHEA
                     HFVGADDAHGAPIMLAAEKAGKAPDAFVAEIARGRAQYLEGFHIGFDNWHSTHSPENT
                     ALSQDIYRRLKRANLIDIRPVEQFYDPVKNMFLADRYIKGECPNCGAKDQYGDACENC
                     SAAYAATDLKNPYSTLSGAKPVLKTSEHHFFRLSDPKCKAFLKDWLERPGRLQPQVVN
                     KAREWLDGEGDKALGDWDISRDPPYFGIRVPDIAEEKYLYVWLDAPIGYLASFKNYCA
                     KAGIDFEKFLQDPGTEQIHFIGKDIIYFHTLFWPAMLEFAGKPYRVPDHVYVHGFITV
                     SGDKMSKSRGTGISPLRYLELGMNPEWLRYYIAAKLNANVEDIDFNPGDFVARVNSDL
                     VGKYVNIASRAASFITRHFDGRLHDFGATPAPAWAAAVAIGDDIAAAYEGREFGRAVR
                     EVMAVADRVNQGFDQARPWELAKDADKRDELHRACSDAIHAFFALTVYLAPLLPATAA
                     RVARELFGLERDLSFEDRKLRATRIRPFKHLLTRVDDRQLDALFDVASVPQPAAPQRH
                     AETQQHAAKENAVPDPISLEEFTKIDLRVARIAKAEYVDGADKLLKLTLDLGGTTRQV
                     FAGIKSAYEPQKLEGRLAVVVANLAPRTMKFGVSEGMVLAASGDGAGIFLISPDSGAV
                     PGMRVK"
     gene            15820..16905
                     /gene="apbC"
                     /locus_tag="AC16O9_15445"
     CDS             15820..16905
                     /gene="apbC"
                     /locus_tag="AC16O9_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR6518049.1"
                     /translation="MAISEADVKAALGALIDPNTGKDFVSTRSVKAIRVAGADVEVDI
                     ELGYPGRSQFEPIRREVIAALKKSGAASANVKVVAKVVPHSVQRGVKLVPGIKNIIAV
                     ASGKGGVGKSTTAVNLALALAAEGAAVGVLDADIYGPSQPTMLGIAGRPESKDGKSLE
                     PMVGHGLQAISIGFLIDVDTPMVWRGPMVTQALEQLLKDTRWRELDYLVVDLPPGTGD
                     IQLTLAQKVPVTGVVIVTTPQDIALIDARKGLKMFEKVGIPILGVVENMSLHVCPKCG
                     HESHIFGSGGAARMCKEYGMELLGQLPLDESIREQADSGKPTVVSDPEGRAAEIYRRI
                     ARRCAVKIAESQKDMTSKFPNIVVQNT"
     gene            17210..18418
                     /locus_tag="AC16O9_15450"
     CDS             17210..18418
                     /locus_tag="AC16O9_15450"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HIR6518050.1"
                     /translation="MGDRPGSLARNRQQTANPQEQLFDTHPEVSLDFEVVRQAARQKY
                     TRPFLILDTAIVREKLRRFRAAMPRVRPHYAVKANPDRRVLKVLAQEGAGFEIASTAE
                     LDQLLSLGVPAAEVFFSNPMRSRDADAYAVAKGVRWFVVDSVDELKRMHAVDPTACLY
                     LRIATPNIGSDWPLSGKFGAGAAEAREIIATASKLGADLGGVTFHAGSQCRNPENWRV
                     GMEKARTLFDAMAKAGLKPRLLDIGGGFPVRHVKPIPSIEVIGAVVNEALQAFPDDVQ
                     VIAEPGRYLVSDAGYFVCRVLGTAMRAGKRWMHWDAGLFGGVIETTEGLKYKIRTERS
                     GPDIAWNVAGPTCDSVDVVMRDEPLPSDLQEGDFIYIRNAGAYTTAYASGFNGFPLPE
                     VRVFESRSRS"
     gene            complement(18349..18900)
                     /locus_tag="AC16O9_15455"
     CDS             complement(18349..18900)
                     /locus_tag="AC16O9_15455"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6518051.1"
                     /translation="MSHPAGTRVYLVAAVASNGVIGSGGKLPWRLPEDLRHFKELTLG
                     HPVIMGRKTWESLGKPLPGRENIVVTRAPGFDAPGAHVAGSLEAALALCAGEPLAFVI
                     GGGELYAAALPLADAMVLTEIGREFPGDTNFPPYDRSAWRETQRKPHTAADGLRFDFV
                     LYERERDSKTLTSGSGKPLKPLA"
     gene            19002..20423
                     /locus_tag="AC16O9_15460"
     CDS             19002..20423
                     /locus_tag="AC16O9_15460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR6518052.1"
                     /translation="MLVTGFLLVALPLAGALLYSAWSTQRLAEQSRHAVYNAAQAARG
                     SRSLVNRIASVERLAQQVAVLPDAGLLQDYERLHEGFQLLTADLARLPLDVQQLAALD
                     RTVAREQVLYELLAAAPQAPPDIATVRARVGELAESAHEVLAISARVADQEVEQLRSS
                     AEIVQRRLLNLVLVAIAVALVAALALTRVIARPIAELDAGIRQLGGPDFSAPIRVSGP
                     QDLRDLGERLDWLRQRLTDLEAQKNRFLRHLSHELKTPLASLREGAELLNDEVAGPLA
                     PAQRAVVGIMRDNSVKLQRLIEDLLDYQRALHAAASLEVSTVQLDALVREVADAHRLS
                     ALAKGQNVALELAPAVVEADAVKLRSIVDNLFGNAVKFTPAGGTITVRAHEADGKAVI
                     EVIDSGPGVPLVEREAVFESFFRGRNKAGGRVAGSGLGLAIAREYVEAHGGRIALVAE
                     GEGGHFRVTLPKRPALLAMEEAT"
     gene            20420..21004
                     /locus_tag="AC16O9_15465"
     CDS             20420..21004
                     /locus_tag="AC16O9_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518053.1"
                     /translation="MRVAALLCAALLAGCALLRPDAREVVQVREIVGAAVSAAHAPAA
                     EQRRQLVHAEQTYVAVPDEVNRVRLAALLATLPAPWRDEARAASLLEPLATRRADTPV
                     AQLAGMLAANVAERQRLARELRAAEARTSAAESRAAAAESRVAAAESRAAAAERREEA
                     AAARAGVLQHQVDALKAIERGILEREERRRKQQR"
     gene            21009..22367
                     /locus_tag="AC16O9_15470"
     CDS             21009..22367
                     /locus_tag="AC16O9_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6518054.1"
                     /translation="MSHEAPSILLVDDDRGLLQLISMRLTAAGYRVTAVEAAEAALAS
                     LAVQRPNLVITDLRMQGMDGLGLFDAIHRQAPSLPVVILTAHGTIPDAVAATRRGVFS
                     FLTKPFEPKVLLETVADALRLSTAPSGDRAHWRSEIITRSAAMEALLGQAQRVAASDA
                     SVCIFGASGTGKELLARAIHRASRRAEAPFIAVNCGAIPEGLLESELFGHKRGAFTGA
                     VQDRRGLFQACEGGTLFLDEVGDMPPALQVKLLRALEDRVVRPVGANDAVPIDVRIIS
                     ATHRKLDERIATGEFREDLFYRLNVVKLELPSLAQRREDIPLLGGHFLARLAERYGRP
                     RLGISPEAMEQLVSAAWPGNVRQLLNVIERAVALTTTEMISASLVRQALDVGAGSLTP
                     LDEARRAFEREYLVRILKITGGNVTQAARLAGRNRTEFYRLLERHALEPGMFKTKVAA
                     ES"
     gene            22566..22892
                     /locus_tag="AC16O9_15475"
     CDS             22566..22892
                     /locus_tag="AC16O9_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518055.1"
                     /translation="MEHTKGKFEIPILAQVAGGFAISALVLIGISGSIYKLVAPQGWV
                     VQAFGHSFGLGAALLAALLGAAAVVWAVPGYMPVQRARLADAVVYGFAAAGLLYLGQM
                     LALGGF"
     gene            complement(22967..23779)
                     /locus_tag="AC16O9_15480"
     CDS             complement(22967..23779)
                     /locus_tag="AC16O9_15480"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6518056.1"
                     /translation="MSPDSGERQYLDLLAEVLERGAAKSDRTGTGTRSVFGRQMRFGL
                     EERFPLLTTKKLHLKSIIVELLWFLRGDTNVRWLQERGVTIWDEWADENGELGPVYGY
                     QWRHWRTADGREIDQIRQVLDSLRTRPDSRRHLVSAWNPADVDKMALPPCHALFQFYV
                     AGGRLSCQMYQRSADLFLGVPFNIASYALLTLMVAQVTDLRPGEFVLTLGDAHLYLNH
                     LEQAREQLGRAPRPFPRMRLNRAVRDLFGFRYEDFTLEDYDPHPAIRAPIAV"
     gene            complement(23776..25026)
                     /locus_tag="AC16O9_15485"
     CDS             complement(23776..25026)
                     /locus_tag="AC16O9_15485"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6518057.1"
                     /translation="MRFLIVDPSRDLRGAVAGMLRARWPDATIEEWDPRERGSPEAAP
                     KLTSYAAILMYVEPTASDALAWMAGMLRDPAAPPVILLTESGGENLALQGLKAGAADF
                     LRKPDLTGERLVRSIEDAVREQEARRTEAANPMAPFERTVKLDAQRIGAPLEGDERRI
                     PGYRSLRKIGEGGMAQVYLAERERDGLQLVLKVLDAKLRADETFFKRFVREYRLIAGL
                     QNEYVAHIYDQGFAGEHPFIAMEYLAGGTLAVRIHEGMTSLTALRITSQVARALGAIH
                     ERGVIHRDLKPPNILFRDNGRPVLVDFGLAKNLSAKTTLTRHGMVIATPRYMSPEQCL
                     GRSADHRSDLYSLGVIFYEMLSGRRLYEGEGPAGLVYQHVHGPIPRLPARLAGYQGIL
                     DRLLAKTPAERFQSAHELFAMIAI"
     gene            complement(25026..25688)
                     /locus_tag="AC16O9_15490"
     CDS             complement(25026..25688)
                     /locus_tag="AC16O9_15490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6518058.1"
                     /translation="MTPLLEAIEIESAPSPDAAVLWLHGLGADGHDFEPVVPQLQLPR
                     ALRVRFVFPHAPVRPVTLNMGMHMRAWYDIAQLGGGAEDAAGIRASQAQLEALIARER
                     ARGVATGRIVLAGFSQGGAIALHTGLRQAERLAGILALSTYLPLAGTVATEHAAANLG
                     APLFMAHGRFDDIIPIERARQSRAALDALGCKVDWHEYPMPHAVCAEEIADIAAWLRA
                     VL"
     gene            complement(25685..26242)
                     /gene="mog"
                     /locus_tag="AC16O9_15495"
     CDS             complement(25685..26242)
                     /gene="mog"
                     /locus_tag="AC16O9_15495"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HIR6518059.1"
                     /translation="MSSVLVVGVVSASDRAAGGVYKDEGIPALKDWLGRAIVAPGVRY
                     EERLIPDERAVIETALVELVDARGCHLVLTTGGTGPAPRDVTPEATLAVADKVLPGFG
                     EQMRAVSLKYVPTAILSRQVAVVRRQALIINLPGQPKAIKETLDGVFAAVPYCIDLIG
                     GPYIETDEAVVKAFRPKSAQRPRPA"
     gene            complement(26235..26717)
                     /gene="yjgA"
                     /locus_tag="AC16O9_15500"
     CDS             complement(26235..26717)
                     /gene="yjgA"
                     /locus_tag="AC16O9_15500"
                     /inference="COORDINATES: protein motif:HMM:NF016626.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR6518060.1"
                     /translation="MRDDTVSKTRRKQEMHELQALGAELVALSAAHLERMTLPAELAR
                     AVHEARSIDSHEARRRQVQYIGKLMRGVDAAPIRAQLAAVHGGSAEERARHQRLEHWR
                     ARLIDDDDALTEFAQAHPSGDLQQLRALIRNARREQAAGRPPRAFRELFRVLREAAGE
                     "
     gene            26740..28095
                     /gene="pmbA"
                     /locus_tag="AC16O9_15505"
     CDS             26740..28095
                     /gene="pmbA"
                     /locus_tag="AC16O9_15505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HIR6518061.1"
                     /translation="MKSGGRFTYTQDQLKALAAQVLAHARRLGASACECDVSEGQGLS
                     VTVRKGAVDTIEHNRDKSIGVTVYLGERPAARRGHASTSDFSAPALAQTVEAALAIAR
                     HTAVDDCAGLPEPELLARETPDLDLYHPWDIGTEEAIGIARRCEAAGFALSPRIRNSE
                     GASVSAQHAQFVFANSHGFMAGFPTSRHSLYVALIAEDARGMQRDDWYSAARVPQALA
                     DAEALGRYAGERALARLGARRIGTRRAPVLFEAPLATQLLGSFVSAASGGSLYRKATF
                     LADSLGTAVFAPGITIEERPLEPRALASSPFDDEGVATRARTVVRDGVLEGYFLGCYA
                     ARKLGMTSTGSAGGNHNLVLASNGPDFRGMLARLHRGLLVTELLGHGVNLVTGDYSRG
                     AAGYWVEGGEIAYPVEELTIAGNLREMFRGIASVGGDVVVRGSRRSGSVLVQNMTIAG
                     E"
     gene            28230..28430
                     /locus_tag="AC16O9_15510"
     CDS             28230..28430
                     /locus_tag="AC16O9_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518062.1"
                     /translation="MAVAILLILWARFEVHGTKDLEEALSEDAAPASAVKPKPSPEAD
                     RSHGSKVEEAVAAGRWAAGFSP"
     gene            complement(28446..29075)
                     /locus_tag="AC16O9_15515"
     CDS             complement(28446..29075)
                     /locus_tag="AC16O9_15515"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6518063.1"
                     /translation="MGANVAAVAKQSENQAERVESVILIAELRNFTHMSEMLEPQRVL
                     GLADQFFECAAGAVAKNGGRSMAVHNDSLMSVFHGAAINALARSAVRAAQQMFGDFDA
                     IVQAWEREYGLRTAIALGVHRGEAVYGEAGPGAQRNAVVFGDCVSITERLVHRARAGE
                     LVLSDALMKVISVAELELDAEPLPPLELHNRPSIRIYGVLRDDRLDFTA"
     gene            complement(29202..29813)
                     /locus_tag="AC16O9_15520"
     CDS             complement(29202..29813)
                     /locus_tag="AC16O9_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6518064.1"
                     /translation="MEMSGEERIPATQDQTWAALNDPEILKACVPGCESIERVAENEY
                     QVLMVARVGPVSAKFKGKLQLSDLNPPVSYAIAFEGQGGAAGFGKGSAQVQLAPEGDG
                     TLLKYEVKASVGGKLAQIGSRLVDAAAKKISKDFFAAFNEKVGAQHGAAGADTDAHDA
                     HEAHASGPVPPDPDLPKVSNTSLIFLAAGALVVLVVGMVVFLA"
     gene            complement(29804..31015)
                     /locus_tag="AC16O9_15525"
     CDS             complement(29804..31015)
                     /locus_tag="AC16O9_15525"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6518065.1"
                     /translation="MSPQLESAAGRGKLSENVMHFARLLRAAGLRVGPDRVIDTVRAL
                     RIAGAERREDWYWTMSSVLLSREEQRPIFDQAFRLFWRDPKLTEKMMQMLLPKTEGRT
                     PKPEEQQSQRLTDALLGQNKPEEEREERKIELDARLTFSSREMLQRMDFDTMSAAELA
                     EAKKMMRELRLPLPMVRTRRLAPHRRGTRVHLRATMRESLREGGDIIPLVRARTRVVH
                     PPLVVLCDISGSMNPYARMFLHFLHAITNDRDRVHTFVFGTRLTNVTRQLRHRDVDVA
                     MAKVADAIKDWSGGTRIGVCLREFNWRWARRVLGQNACVLLVSDGLDREAGEGLAEEM
                     ERLAKASHYLVWLNPLLRYERFEAKPAGVRAMLPHVDLFLPVHNLKSLVELARTLSRP
                     LARPMEASAWK"
     gene            complement(31017..31931)
                     /locus_tag="AC16O9_15530"
     CDS             complement(31017..31931)
                     /locus_tag="AC16O9_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6518066.1"
                     /translation="MPQPLPASIDQTQTLLAAADYVADRSLATALYLSLAMQRPLFLE
                     GEAGTGKTEIAKVVAQSLGRELIRLQCYEGLDIAQAAYEWNYPRQMIEIRLAEAAGEK
                     SKDKMSHDIFDARFLIKRPLALALEGKPGAAPVLLIDELDRTDEPFEAYLLEVLSDWQ
                     ITVPEIGTLRAAEPPIVVITSNRTREIHDAVKRRCFYHWVDYPDARRELEILKKKAPG
                     AAQALSREVVAFVQRLRGVDLFKLPGVAETIDWANCLVALDRIALDPETVNNTLGVLL
                     KYQDDIEKIAGDEATRLVAEAKSAAAAA"
     gene            complement(31940..32431)
                     /locus_tag="AC16O9_15535"
     CDS             complement(31940..32431)
                     /locus_tag="AC16O9_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR6518067.1"
                     /translation="MREHRTLRWLGPLLDRPGLWHFDRRSVALGAALGVFFGFMIPVA
                     QILASALVALLLRANLPVAAVSTLVSNPLTYGPIFVLAHRTGTALLGQVPDPVEQAHV
                     EEQARAPIDDPKTWGERFGAIGKPLALGLAVFAFVGGIIVWVLVNLLWLLSVRLRPRA
                     PPR"
     gene            complement(32466..33263)
                     /locus_tag="AC16O9_15540"
     CDS             complement(32466..33263)
                     /locus_tag="AC16O9_15540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6518068.1"
                     /translation="MHAFNYHRPANVNDAAALAAKSADAKLLAGGQSLVQAMKLRLAA
                     PTDIVDLGMIKDLAGIKVDGKTVIIGAMARHAEVAGSAEVKKAIPALAALAGMIGDRQ
                     VRNMGTLGGALANSDPAADYPAAVLGLAATITTTKGTHAADKFFKGLYETALKPGEVI
                     TSVSFPIPKRAAYAKFKNPASRFAIVGVFVADMGNGVRVAVTGAGPCALRLAEMEKAL
                     TAKFAPDSVANLKVKADGLNSDLHASAEYRAHLITVMAKRAVEAALK"
     gene            complement(33274..35628)
                     /locus_tag="AC16O9_15545"
     CDS             complement(33274..35628)
                     /locus_tag="AC16O9_15545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6518069.1"
                     /translation="MGAPLIGARVERKEDYRFLTGAGQFTDDVTLPRQSYAAFVRSPH
                     GHAKIKSVNTAKAKAAPGVLDVFTGADIAAAKLGGLPCGWLITDVNGQPMKEPPHPVL
                     AQGKVHHVGEQVAVVIAETQDQAQDAAELVQVDYEVLPAVADVRLARAKGAPQLHEIA
                     PNNTCYVWALGDKAAVDAAFANAAHVTKLDFVNNRLIPNAIEPRAANGSYSRADDSYT
                     LYVANQNPHVERLLMTAFVMGLPEHKVRVVAGDVGGGFGSKIYLYAEDVVVTWASRQL
                     GRPVKWTADRSESYVSDAHGRDHHTVAELALDKDGKFLAMRVKTTANIGAYLSTFASC
                     VPTILYATLLSGQYTTPLIHCEVTAVFTNTAPVDAYRGAGRPEAAYVVERLVETAARE
                     MKIDPAEIRRKNFIRQFPYQTPVALQYDIGNYDATMDAAQKLADVAGYAARKKASEAK
                     GKLRGLGYAAYIEACGIAPSAVAGSLGARAGLFEAGEVRVHPTGSVTVFTGSHSHGQG
                     HETTFAQVVADRLGLPIENVDIVHGDTSKILFGMGTYGSRSIAVGGTAIVKALDKIIA
                     KGKKIAAHLLEAAEGDIEYANGVFKVSGTDKQKMFGEVAFAAYVPHNYPHDKLEPGLN
                     ENAFYDPSNFTFPAGTYVCEVEVDPDTGVVRIERWSAVDDFGKIINPMIVEGQVHGGL
                     VQGIGQALTEGAKYDSTGQLVTGSFMDYCIPRADDVPSFQVDTRETPCTHNPLGVKGC
                     GEAGAIGAPAALINAITDALGVKDIAMPATPQTVWRTLQQKKAA"
     gene            complement(35639..36118)
                     /locus_tag="AC16O9_15550"
     CDS             complement(35639..36118)
                     /locus_tag="AC16O9_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6518070.1"
                     /translation="MAIKVSMKVNGKAVSGEVEARTLLVTFIREQLRLTGTHVGCDTA
                     QCGACTVQMNGKAVKSCSMLVMQAEGADILTIEGLAKADGTLHPMQAAFKEHHGLQCG
                     FCTPGVVMNAVDFAKRHPNASEQDVREALEGNLCRCTGYHNIVKSIMAGSKAMTAAK"
     gene            36235..37242
                     /locus_tag="AC16O9_15555"
     CDS             36235..37242
                     /locus_tag="AC16O9_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6518071.1"
                     /translation="MDSVDIEVLRSAEAWRKSGHGVTLGTIVKTWGSAPRPVGALVAI
                     RDDGQVAGSVSGGCVEDDLIEKVREKALAKDAPQLVTYGVTNEEATRWGLPCGGTLQV
                     VLEPVSEASGLGGLLDTISQQQLVRRRLDMDSGRVTVEPGRWSDALEFDGKVLSTVHG
                     PRWRLLLIGAGQLTHYLAEMAKMLDYHVSVVEPREEYAAGWNVPGVKLDRRMPDDVIA
                     ESNLDGHSAVVALTHDPKIDDLALMEALKSAAFYVGAIGSKKNNDARRKRLLEFDLSQ
                     AEIARLRGPVGLYIGSKTPPEIAVAIMAEMTAVRHGVTDPSWAAKPQPRFDPSACEVK
                     T"
     gene            37239..37817
                     /locus_tag="AC16O9_15560"
     CDS             37239..37817
                     /locus_tag="AC16O9_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6518072.1"
                     /translation="MKLVGLLLAAGSATRFGSDKLQHPLPHGVSIAVQAARHLKAEVP
                     RVIAAVRPDGLATARALAAEGCEVVVCERAADGMGASLACAARAAGAVDGYLVALADM
                     PFVRRSTIAAVREALERGALLAAPYWRARRGHPVAFAGRLRGELEALAGDEGARRVLD
                     AHAAALVKVPVGDPGAVRDIDQPSDLAPPLAV"
BASE COUNT         5781 a        12986 c        13297 g         5872 t
ORIGIN      
        1 gcggctgtcc tcgaagtctt cgaagcggcg gccgagcgct gcgagccact catcgacgcg
       61 ccggagcttg ccttcgcgaa agcacggcgt gccggcgagc cgcccggtga agcggccggc
      121 gatgcgctcg ggctcggtgg caacgaggtg cggtacgccg agctcgcgcg cgatgggtgc
      181 ggtgacgaag ctgttggtcg cggtgaccac cgcgcacagc tcgcccgcgg cgaggtgccg
      241 cgccacgagc tcgagcgcgc gcggcagcag cattggccgc acgcgctcgc gcatgaagtc
      301 ctcgtgccag cgcgccagat cctcgggcgt atgcgcggcg agcgggccga gcgcgaagcc
      361 caggtactca tggatgtcca gggtgccggc cttgtactgc tcgaagaagc gcgcgttctg
      421 cgcctcgtac tcggcgcgcg acagcacgcc gcgtgcgatg aggaactggc cccactcgta
      481 gtcgctgtcg ccggccagca gggtgttgtc gaggtcgaac agcgtcaacg tcactgctcg
      541 aacgccttca gcgcttcgcg taccagcggc agcgtcacgg ggcgacgctg ctcgagcgaa
      601 gcgcggtcaa gctgctcgag cagtgcgcgc agcgacggca tgtcgcgctt gacgtgcgtg
      661 agcaggtacc agaccacctc gtcggcgagc cgcaggccgc gccgcgccgc ctcggcacgc
      721 aggtatacgg cgcgctcggc atcggtgagc ggcttcactt cgtaaaccag cccccagccc
      781 aggcggctgc gcaggtcctc gcgaagcgcg agctgcgccg gcggcgccgc gcccgcggcg
      841 agcaccgtgc cgccgtcttc gcgcgcccgg ttgatggcgt tgaacagccc ctgttgccct
      901 gccgcgtcga gggcctcgac atcgtcggcg agggtgacgc cgggccgtgc gctcgccgcg
      961 agcaggtggc tgcgtccgca gccggccgcg ccccagagat agattacggc ctcgcgcaag
     1021 cgccccgcgg cgagctcgcg caggcgctcc agcagctcgc cattggcgcc cgggacgaaa
     1081 ttctccagcg tcggcaccgg cggcgccgag agcggcagcg ccagctggcg cgtcacttcc
     1141 cgccgccgta caacgagctc gccagatatc cctcgcgcac gtgtcgcagg gcgacgagca
     1201 gcgccgcgct ggcgggcagc gccatcagca cgccgaaaaa gccgaacacc tgcccgaagg
     1261 cgagcagcgc gaagatcacg gctaccgggt gcaggccgat acgctcgcca accagccacg
     1321 gcgtcaccac catgccctcg agtgtctgcc cgacaccgaa cgcgagccac acccaggcga
     1381 ggccccacag gctgtcgaac tgcatgagcg cagtcaccgt gccgagcgcg aagccgagca
     1441 gcgcgccgac gtacggcacg aacaccagca ggccggtgat caggccgatc ggcaacgcga
     1501 actccagccc ggcgagccac agggcgagca cgaagtacac gctcatcacc aggatcacgg
     1561 cgagctgccc gcgcaggaac tcggcgagca ccgaatcgac ttcgcgcaga attgtgcgcg
     1621 tcctctggtg caggtggcgc gggatcagac ggtcgacgcg cgccagcatc tggtgccagt
     1681 cgcgcagcag gtagaacagc accaccggca cgagcagcag gttgacgagg aacgcgagaa
     1741 gcgcgaggcc gccgatgcgc agcgatccca ggagcttgcc gaccaggtcc tggttttcgg
     1801 ccacgacctc cgtggccagc gcgcgtacgc tcgccacgtc gagctgcagc gcaccgccga
     1861 aatagcgctc tgcgaacggc accagcttct ggttcagcca ggcgatgaac gcgggcagcc
     1921 gctcggcgag caggcgcagc tccttggcga acagcggcag cacgacgagc gcgagcgcga
     1981 cgaggagcgc cagcacgccg ctcatcacca ggactaccgc gagcgtgcgc ggcacgaact
     2041 tgcctgtgag gcgctcgacc agcgggtcga gaatgtaggc gaggatcgcg gcgaacagga
     2101 acggcgcgag aatgggcgac aacaggtaca gcagcccgcc caccaccagc gcgacagcga
     2161 gccagcccca gagatgcggg ttggcggaga gcggcatggg catctaaagt agaatcgcgc
     2221 ggaaaacgag aaactctagc atggccgacc cgctttccta ccgcgacgcc ggcgtcgaca
     2281 tcgacgccgg cgatgcgctc gtcgaggcca tcaagccatt tgcccggcgc accatgcgcc
     2341 ccgaagtgat ggcgggcatc ggcggcttcg gcgcgctgtg ccgaatcccg aggaagtacc
     2401 gccagccggt gctcgtatcc ggcaccgacg gcgtgggcac caagctcaag ctcgcgtttg
     2461 ccttcgggcg ccacgacacc gtgggcatcg acctggtggc gatgagcgtg aacgacgtgc
     2521 tggtgcaggg cgccgagccg ctgttcttcc tcgattactt cgcctgcggc aagctcgaac
     2581 cgcgcgtggc ggccatggtg gtgaagggca tcgcgcgcgg ctgcgagctc gccggctgcg
     2641 cgctgatcgg cggcgagacc gcggagatgc ccggcatgta tcctgcgggc gagtacgacc
     2701 tggcgggatt ttgcgtcggt gtggtggaaa agcgtcgcat catcagcggc cgctcgatcc
     2761 ggcccggcga cgtgctgctc gggctcgcct cgagcggcgc gcactcgaac ggctactcgc
     2821 tgatccgcag gatcctcgag cgcgaggcgc cggacggcgc gctcaccgac gccttgatgc
     2881 agccgacgcg gatctacgtg cgatcgatcc tgcagctcct gcgcacggtg aacgtgaagg
     2941 ggctcgcgca catcacgggc gggggcctgg tgggcaacgt tccgcgtgcg ctgccgccgc
     3001 gcactcgcgc ggtgatcgac gcccgcgcct ggccgcggcc ggcgctgttc cagtggctgc
     3061 agcagaaggg cgccgtcgac gaggccgaga tgcaccgcgt cttcaactgt ggcatcggca
     3121 tggtcgcggt ggtcgccgcg agcgacgccc ggcgcgcaac gaagcgcttg cgcgccctgg
     3181 gcgaaacggt ctacgagatc ggcctgatcg aaagggcccg gggcgaagcc gaggccgtcg
     3241 tcagctagtt accttgtaga ccacgtcgtg ctcgcgcacg tgctggtcga cgcgcacgcc
     3301 gaaatcgtct tccggcccca cttcgttcac cggcgcgtgc tccacctgca gcgaggcgac
     3361 gcgctggctg aagtcggtgg tgtgtccccg gatatggatc gtgtcgccga cgcgcagcat
     3421 gccggtctcg agcttcacga tggcgacaga aggctcgcca tagaaatgcg tcacgacccc
     3481 gatgcgctca cccggcgcgg cgggttcggg cgccgccgcc ggccgcggct cgggcgcccg
     3541 gcgcttgacg atcttgcgcc tggcggcttt cggcgccgcg cgcctgaccg gcgccttgcg
     3601 cgcgggcttg cgcgcgcgct ttacgacctt cctgcgcgcc gccggccgct tggccagtgc
     3661 cttgcggcgc ttgggcttgg ccttcgcttt cttccgggtt gccatgcgta tcctcctcgc
     3721 ctgaggtgac gcgctattct gtccccaggg aactgcggcg cgcaagatag atccccgcca
     3781 gcacgacaac cccgccggcc acctgcaggg ccaccagcgg ctcgccgaga agcacccagg
     3841 cgaatgcagc cgccatcacg ggctggaaca gcaggccgac ggaggagaaa gccgccggca
     3901 ggtgcgcgag cgcgtaggcg atgagcccct ggcctcccgc gtgcgtgatc caggccaggc
     3961 cgagaagcac cagccagccg ccggcaccgg ccggcagcat gacctcgccc gaggcgagcg
     4021 ccgcgggcag cagcaccagg gcggtcacgg tgctggtcac cgccatcaac cgcagcgtcg
     4081 ccgcgccacg atcgcgcagc cctttcaccg tgagcaggta ccaggcgtag aacatcgccg
     4141 tgaccacgcc cagggcgtcg cccagcagcg cgcgcggcgc gaagtccacg ctggtgcgta
     4201 ccagcatgcc gacgccgacc agggcgagcc ccagcgcgac caggaacagc gcgcgcggct
     4261 tctgccgcca cagcagccat gccgccagcg tgacgaagat cgacgcgaga ttggcgagca
     4321 gcgtggaatt ggcgaccgag gtgaactgga tcgaccagtg ccagaacgcg agatcgccgg
     4381 caaaggcaac tcccgcgacg ccgagcatgc gccagtccgc gcgccgcgcg ccgcgtgcgc
     4441 ccggcgcgag cgcgtacgct gcccagagca gcggcacggc gagcgcgacg cgccagaatg
     4501 cgctcgcggt cggcccgacg tcggcgagcc gcacccagat cggcgacagc gcgatgaacg
     4561 tcgcgccgca gagcagggcg agcagggccg gagcgcgcat gcgcggatgg taaactttcg
     4621 ctgcttccca tggctgcacc tcgcttcgtt catctcaggc tgcacagcga gtactcggtg
     4681 acggacggca tcgtccggct cgatgcggcg gtcgggcgcg ccgcgcgcga cggcatgccc
     4741 gcgctggccc tcaccgacag cgccaacgtc tttggcatgg tgaagttcta ccaggcggcc
     4801 cgcgccgcgg gcgtgaagcc gatcgtcggc gcggactgct gggtgcaggg ggcggccgaa
     4861 gccgacaagc cggcgcgggt cctcctgctg tgcgcctcgc gcaccggcta cctgcgcctg
     4921 tgcgagctgc tgtcgcgcgc ctggctgcag aaccagcgcc aggggcgcgc ggagattgcg
     4981 cgcgcgtggc tcgccgagtc cgataacgaa ggcctgatcg cgctctcggg cgcgggcgcg
     5041 ggcgacgtgg gcgccgcgct cgccgccggc aacgcggccg ccgccgagcg gctggcgcgc
     5101 gaatggtcgg cgcgctttcc ggggcgcttc tatctcgaac tgcagcgcgc gggcttcgcc
     5161 cacggcgaga cgctggtggc gcgcagcgtc gagctcgccg cgcgcctcgg cctgccggtg
     5221 gtcgccacgc acccggtgca gttcctcgac cccgaggact tcaaggccca cgaggcgcgc
     5281 gtgtgcatcg cccagggcta cgttctgggc gaccagcgcc ggccgcggca attcacgccc
     5341 gagcagtacc tgaagtcgca ggacgaaatg gcgcggctgt tcgccgacct gccgcaggcg
     5401 ctcgacaacg cgctcgagat cgcacgccgc tgcaacctgc agatcgagct cggccgcagc
     5461 cgcctgccgg cgtttcccac gcccccgggc gtgacgctgg accaatacct gcgccggcag
     5521 gccgagcagg ggctggcgac gcggctggcg aagctctttg ccgacgcggc cgagcgcgcg
     5581 cgcgccgcgc cgcactacca ggagcggctc gatttcgaga ttcgcaccat tatccagatg
     5641 ggcttcgcgg gctacttcct catcgtcgcc gacttcatca actgggccaa gcagaacggc
     5701 gtacccgtgg ggccgggccg tggctcggga gcgggctcgc tggtggccta ttcgctcggc
     5761 attaccgacc tcgacccgat cacctacaac ctgctgttcg agcgcttcct caacccggag
     5821 cgcgtgtcga tgccggactt cgatatcgac ttctgccagg acgggcgcga tcgcgtcatc
     5881 gactacgtac gccagaagta cggcgcggac agcgtgtcgc agatcgcgac cttcggcacc
     5941 atggcggcgc gcgcggtggt gcgcgactgc ggacgcgtgc tggacctggg ctacaacttc
     6001 tgcgaccagg tggcgaagct gatcccggtg cagcccgggc gcaccatcac gctcgaggat
     6061 gcgcgcagca tggagccgct gctcgcgcag cgcgagaaga aggaggacga ggtgcgcgag
     6121 ctgctcgcgc tcggcgagaa gctcgaaggc ctgacgcgca acgtcggcat gcatgcgggc
     6181 ggcgtgctca tcgcgccggg caagctcacc gacttctgcc cgctgtatgc ggccgagggc
     6241 ggcgtcaacg tgatctcgca gctggacaag gacgacgtcg aggcggtggg actggtgaag
     6301 ttcgactttc tcggcctcac gacgctcacc atcctcgact gggccgagcg ctacgtgcgc
     6361 cagatgggcg acgatcagtt cgcccttgac cgtatcccgc tcgacgacgc agcgacgtac
     6421 cagctgctct cggccggcaa caccaccgcc gtgttccagc tggaatcgcg cggcatgcgc
     6481 gatctgatca agcgtgcccg tccggaccgc ttcgaggacg tgatcgcgct ggtggcgctg
     6541 taccggcccg ggcccatgga cctgatcccg gacttcatcg cgcgcaagca cggcagccag
     6601 cgcgtcgagt acctcgatcc gcggctcgag ccgatcctgc ggccgaccta cggcatcatg
     6661 gtctaccagg agcaggtaat gcagatcggc caggtgatcg gcggcctgac cctgggcggc
     6721 gcggacctgc tgcggcgcgc gatgggcaag aagaaagccg aggagatggc gcagcagcgc
     6781 gacgtcttcg tcgccggcgc cgagaagaac ggcttgtcac ggaccaaggc gacgcagctg
     6841 ttcgacctga tggagaagtt cgcgggctac ggctttaacc gctcccatgc cgcggcctat
     6901 gcgctgctgg cctaccagac cgcctacatg aaggcgcacc acgcctccgc gttcatggcg
     6961 gcaaacctct cgctggtgat gggcgatacc gacaaggtgc gccagttcca cgacgatgcg
     7021 ctcgccaacg gtctcacgat acaggcgccc gacatcaacg cctccgagtt ccgctttgtg
     7081 ccggtcgatg aaaagaccct gcgctacggc ctgggcgcgg tacgcggcac cggagagtcg
     7141 gcgatccatg ccatcgtcga ggcgcgcaag gccgggccct ttgccgacct attcgatctg
     7201 tgccgccggg tcgataagcg cgcggtgaat cgccgtgtca tcgaagccct ggtgcgcgcg
     7261 ggcgcgctcg atgcgctcga gcccaatcgc gccagcctgc tggccaccgc gggccgcgcg
     7321 ctcgaggcgg ccgagcaacg cgagcgccag gccgcccagg tcagcctgtt cggcgatgcc
     7381 gccgatgccg acggcgccga gcgcgccctg gtcccggcgc agccgtgggg cctgaagcag
     7441 aagctgaccg aggagaaggc ggcgctcggc ttcgcgcttt cgggccactt gttctcggtc
     7501 tacgagcacg acctggccgg tttcccgcgc gtgccgctgg cgcagctcgc ggcggccgag
     7561 catcgggtgt ggctcgcggg cgtggtggcc tcggcgcgca cgcagatgac gcgccgcggc
     7621 cgcatgatgg tggtgatgct cgacgacggc agcgcgcagc tcgagctgtc ggtgttcaat
     7681 gagctgttcg aaaggcaccg cgacaagctc aaggaggacg cgctgctcat cgtgcagggc
     7741 aaggcgcaac gcgatgaatt ctccggcggg ctgcgcgtga cggcggacga gctctacgat
     7801 ctcggcgcgc tgcgcgagcg cttcgccgcg cgcctgcgcc tggagatgaa tgggcactcg
     7861 gacgcgcgcc agttgatgga gctgctcacg ccgtaccggg cgcaaggggc ctgcccggtc
     7921 ttcgtgcact acgagaccgg cgcggcggcc tgcgacgtcg cgctcggcga tgcctggcgg
     7981 gtgcgtcccg acggccagtt gctcgagcgc ctcggcgcct ggctggcgcc ggagaatgtg
     8041 caggtggtgt acgcgggcgg cgtctagtcc ttcgcccagc gcacgcggca gctctcggcg
     8101 cgccacacgc ggcgcgtccc ccggtaccag gcactcagca cccgctccgc gggatccagt
     8161 cggcgctttt cggcgaggaa ggcgcccagc atcgggtagc aggcctcggc ctgggcgcgc
     8221 gtcgcaagtg gcaggctgcc gggcatgaaa ccgccgccgg ccgcgagcgc cagatcgatg
     8281 aggtgcgtgc gcagttgcgc tgcgcttgcg catgcgccga gcgtggcgcg ggtatcaaac
     8341 tcgatgtgca gagccacata ctcgctgcgc gcccagcgca gaaaggtctc cgcctccgga
     8401 tggacgcagc gcgcctccaa cagcctcaag cagcagcgcc gttcagcgag cgccgcgcgt
     8461 gtccggtcca cgacctcgtc gctgcgctgc ggaggcacca gcagctcgag cgcggtcgac
     8521 tggcctcccg gaggctcgtc ggcaagctcg atgcgcacgg gcgcggccga ccgagctgcg
     8581 cttctcgcca gcgaggccac gtcaagcgtc aggctgtaga acggcccgaa tgccccgaat
     8641 ccccccaccg ccagccggaa caggtccgcc gcgcgctcac ggctgacgcg acgcagttca
     8701 ccggcgcctg ttgccagagt gaaggcatgc agatgcctga cgaccggctc gccgtcgggc
     8761 ccggcccggt tggccgcaac gctttcgcca acgcttccgg gcaggaagtc ggcgccgaca
     8821 tagggccgca ggctgcgcca ggacgcgcct gcctggactt cgagcaggcc gcgcgcggcg
     8881 tcgtggcgca gggtgcgatc gaggcaggcg gcctccaggg gccgggcgta cgcgccttcc
     8941 gcgagcatcg cccggcaaag gtcgctcgcg gagtacacgc gaggtgcgga aagcgtacca
     9001 agggacatcg ggaacgtggc gagcatcggc agcctcggcg ggttagcggt cgacccattg
     9061 tcgaggcggc gcctgttgcg ccgcaataac gcagcggcaa ggtctcgctt tacgataccg
     9121 gcggttccct ggcctgcacc catgccgctc acccaccgca ttgtcgatgc gctcaagcgc
     9181 tccctgcgcg cccgcggcat gacctacgcg gcgctcgcgc gcgagctgcg gctctcggaa
     9241 gcgagcgtca agcggctgtt ctcgcgccat acattcactc tgtcgcgcat ggaggacgtc
     9301 ctgcgggtgc tcgatctcga cctggcggaa ctggcgcgca tgacgcgggg cacgcaaggc
     9361 caggacatcg aactcagcct ggagcaggaa acgctgctgg cgagcgacga gcggctgctt
     9421 tcggtgttct ggctgctgct gaatggctgg cgcttcgacg acatcgtcga agcgttcgtc
     9481 atcacgcgta ccgagctgac gctcgcgctc gccaagttcg agcgcgcgcg cctcatcgac
     9541 tgggatgctg gcgagcgcgt gcggttgcga gtggcaaagg acttcgtgtg gcgcgccggc
     9601 ggaccggtca agaaggccta cggcctgcgc gtgactgcgg agttcctgcg ctctcgcttc
     9661 agcgggccgc tcgagatgct gcgcttcgag gcacgcgacc tgtcgccggc ctcggcggcg
     9721 atgctgcggc gccgcctgga gcgtgtcgcc gccgagttca acgagcttgc cgaagtggat
     9781 gcggggctgc ccgcacgccg gcgcgaaggc gtcggcctgc tgatcgcctg ccggccatgg
     9841 gcgttctccg cgatgaacgc gctgaagcgc cgcgatacgg gccgcgatca ggcggtcggc
     9901 cagcgctcga atcgcgcata gctgcgcgca tgctccggcg cgggggggcc gcaatcgacg
     9961 ccctcgacgc gcgccgcggt cggcccgcgt cgggcccagg cggtcagcgc ctcgagggcc
    10021 gcggcgtcac cctgcgctac ggcctcgaca tcgccgccgg cacggtttcg cacccagccg
    10081 gtgagtccca gccgctcggc ttcgcggctc atggcgtcgc gaaagccaac gccctggacc
    10141 cggccgcgga tgcacagccg gattgtgcgt acttgcaaaa cttctccagc gttcggcgcc
    10201 gctcgctgcg agcgcgggcg ctccatggta agctgaatag agtcttatcc tccaatctgg
    10261 cgcggatgga aagacacggg caaaaggggg cgactcgatg cgtgcagctc taatcgtcgt
    10321 ggcgattctg gctttggcgg catttctcgc ggtcgcggtc gtgctgccgc agatggcggg
    10381 cagcgaggcg cgcgaagccg cgcaggcgtt gatcgcgggc accgagggtg ctcagaagca
    10441 gattgcggcg gcggcgcaga gcgcgggctc gctcaccggc accggcaagg acctgaagat
    10501 gccggcccgc agcgacccca agcacggcga actgaaatgg atcatcgaga caaacggcgc
    10561 catccgcggc tggaacgaga agaatgcgat cgaaatctcg atcacgccga tccttcaggg
    10621 cggcacggtg acgtggaact gccgcggcta cccgatcacg gcgatgccgg ccaactgcgg
    10681 cggcaagtcc tgagcgccgc cgcaaggcgc cgcggcacta caacagcacg ataatcgccg
    10741 cggcgatcag cccgctgatc gcgcccgcgg ccaccaccag cgtatcgcgc agccgccagc
    10801 cgccgctcag ggcggtcgac gtggtctgca tgctgagctg cagcgggtgg ttcgccgccg
    10861 gatctcgggt cagtgccgcg tcgctcagcg ccaccagccg ctgcgtcgcc tcgagcgagt
    10921 cgaaggtacg cgacaggcca cgggccggtg ccgcgtccgg ggccggcgca ccgtcctcgg
    10981 cgcggcgcga gcgctccagg gtctgcgcca ggatggcgct tttcacgtcc ttgtccacca
    11041 ccgtcggtgt cgcgccttcc gcgagcgagc cgagcggcag cgtcgaggtc gcgctggtgg
    11101 ccgccacgcc gtgcacctgt cgctcgcact cgcgcaggcc gcgcgcgact tcggccgcgc
    11161 tctggaagcg ctcctcgagc gatttcgcca gcatgccggc gacaatgccg tcaagaacgg
    11221 cgggcgccgc cgggttgatc gcgctcggcg cgggcggcac gaagttcacg atctgataca
    11281 tcacggcatt gacgctttcg ccgctgaatg gcgtcgagcc agtcagcatc tcgtagagga
    11341 tcacgccgag cgaaaagatg tcggtgcgat gatcggcgcg cttaccgatc acctgctcgg
    11401 gcgacatgta cttcggcgag cccatcatca tgccggtctg cgtctggacg ttggacgagc
    11461 gcatgcgcgc gatgccgaaa tcggtgattt tcactgcgcc ggtttccggc accatgatat
    11521 tggccggctt gatgtcacgg tgcacgacgc cgcgctcgtg ggcgtaggcc aggccttcgg
    11581 ccacctgcgc tgcgatcgac agggcgcgcg gcaccggcat cggcttgcca ggcaggagca
    11641 gcgtgcgcag ctccgcgccc tcgatgtact ccatcgccat gtagcactcg gtgtcggtct
    11701 tgccgatgtc gtacaccgtg acgatgttgg gatgattgag cccgcccgcc gcgcgcgcct
    11761 cctgattgaa gcgcttctcg tacatctcgg cgccgtcgcg gtccatgctc atgttgatgg
    11821 tcttgatggc gacggtgcgc tcgagcatcg ggtccttcgc cttgtagacg atgcccatcg
    11881 cgccgcggcc gatctcggca aggacttcga agcggccgag cttttggtcg tccatcgcgc
    11941 ccattgcggc ggcttagcgg ccggccttga ccgcgcggaa caccgggccc cagatcggat
    12001 gccccacctc ggcggcgctc agctccatgc ccggatcgat ggccagcgcg cggtcgaatt
    12061 cacgccggca ggcggccggt cgccctgaca cgcaatcgat gaacgccaga tacttgtgcg
    12121 cctttacgcg gtcggggctc gagagtccct gatacaggct ggtgttgagg ctctcggcag
    12181 cctcggcgta cttgccttcc tcgtactggc ggatgccgac ggcgagcgcc ggcccgcctt
    12241 tggcgctgcc gaacaggctc ctgaagtcct cctgcagggc gcgcacggac gcgctctggc
    12301 agccgctgaa agccaagccc gcaacgctca gcgcaatcgc cagcctaatt ttcatcccgc
    12361 cagtttccag actgcgccat cgtccatcgg caatcccccg aagctaccgg gcggaagata
    12421 ccgcgagagc acgcttagcg aaagcgatgg cggatgcgca ccggctcgcc cgagcgtact
    12481 tcgacgcgcc gcgtatgggt tggaaacgag gtgttgcgca cctcgacggt gtgcgttccc
    12541 agagggactt ccagcgagcg cagcggcgga ctgacgccgc ggcttttgcc gtctacgaag
    12601 acttcgcccc aaggcaggat cgcgagctgg atggtgccgg cggcagccga cgcggcgatc
    12661 tgcgccggcg cggccgccgg ggcagcttcc ggcttgcgtg cctcgcgcgc cgggcgtggg
    12721 gtgcgggcgg ccagcgtttc gcgcagttgc ccgcgttgcg tgggcgcggc tgccaccggc
    12781 gctggcgtca cgggcggctg cggttgcgcc gactcagcgg cagccggctt ttcgccgttg
    12841 gcgggcgctt cggcgcgcgc aggctccggg gccgccatct gcagcgccgc cggtccgggc
    12901 ttgatcgcga gcggcggatg gctgatctgt gccttgacgg ccaccccccc gctgacgagc
    12961 agcacgaagg cgaggggcgc gccgaggaac gccaagcggt gcacgccgaa cggccgcacg
    13021 tcggtgagct cgccgatcaa ccgagggccg cggcggcgcc gcggcgccac gcggcgcgag
    13081 ctggtctcaa tcaccctgcg cgcggcgtcg gtgccgatgc cgaccgagta cacctcgtgc
    13141 tcgcgcacgt gcttgtcggt gcgcgcgccg acgtagctga acaggctggc gtagtcgagc
    13201 gacaggcagg acaccacctc gtagaaggag cgcgacacca gcagctgtcc cgcctgggcg
    13261 aacgtcatca cgcgctgtgc gacgttgatg ccgtcgccga tgatgtttat ctggccgttg
    13321 atgtccttca cgaggcgcac cgggcccaga ttgatgccga tgcgcacggc gatggcaccg
    13381 ctcgattccg cgcgcgccgc cgcgcgcagc gacatggcga cgaacagcgc atcttcggga
    13441 tcgccgagga aggtaatgcc ggcgccgtcg ccggtgtcga ggacgacgcg atcgcgcggc
    13501 gccacgtttt ccagcgccga gctgagcagc ttgttgaact gctgcttgag caacaactgc
    13561 tcggccactg gcttcgccga gtactcgacg atgtccagga acacgatgct gcaaaccagc
    13621 gtccggctgg gctgttcgat catgcgtgct tcgtgggtcg acccgaaggc cgtaacgcgt
    13681 tcattttacg cgcatacccg gaaccgctcc gctatcgggg gaaatgagaa atatgcccgc
    13741 tccgtcgccg gaagcggcga gcaccatgcc ttcggagacg ccgaatttca tggtgcgcgg
    13801 agcgaggttg gccacgacga cggccagccg cccctcgagc ttttgcggct cataggccga
    13861 cttgatgccg gcgaacacct ggcgcgtggt gccgccgaga tcgagcgtga gcttgagcag
    13921 cttgtcggcg ccgtcgacgt actcggcctt tgcaattcgc gccacgcgca ggtcaatttt
    13981 tgtgaattct tcgaggctaa tggggtcggg cacggcgttc tccttcgcgg catgctgctg
    14041 ggtctcggcg tggcgctgcg gggcggcggg ttgcgggacc gaggcgacgt cgaacagggc
    14101 gtcgagctgc ctgtcgtcca cgcgcgtgag caggtgcttg aacggccgaa tgcgcgtcgc
    14161 gcgcagcttg cggtcctcga agctcaggtc gcgctcgagg ccgaacagct cgcgcgcgac
    14221 ccgtgccgcc gtcgccggga gcaggggtgc aaggtagacc gtgagcgcaa agaacgcgtg
    14281 gatcgcgtcg ctgcaggcgc gatgcagttc gtctcgtttg tccgcgtcct tggccagctc
    14341 ccagggcctg gcctggtcga acccctggtt gacgcgatcc gcgacggcca tcacctcgcg
    14401 cacggcgcgg ccgaattcgc gcccctcgta ggccgcggcg atgtcgtcgc cgatcgcgac
    14461 ggcggcggcc caagccggcg cgggcgtcgc gccgaagtcg tgcagcctgc cgtcgaagtg
    14521 ccgggtgatg aagctggccg cgcggctcgc gatattgacg tacttcccga ccagatcgct
    14581 gttcacccgt gcgacgaaat ccccgggatt gaaatcgatg tcctcgacgt tggcgttgag
    14641 cttcgccgcg atgtagtagc gcagccactc cggattcatg cccagctcga gatagcgcag
    14701 ggggctgatg cccgtgccgc gggacttgga catcttgtcc ccggacactg tgatgaagcc
    14761 gtgcacgtag acatggtcgg gcaccctgta gggcttgccg gcgaactcca gcatcgccgg
    14821 ccagaacagg gtgtggaagt agatgatgtc cttgccgatg aaatggattt gctcggtgcc
    14881 ggggtcctgc aggaacttct cgaagtcgat cccggccttg gcgcagtagt tcttgaagct
    14941 tgccaggtag ccgatcggcg catccagcca gacgtacagg tacttttcct ctgcaatgtc
    15001 gggaacgcgg atgccgaagt aaggcgggtc gcgcgagata tcccagtcgc ccagggcctt
    15061 gtcgccctcg ccgtccagcc actcgcgggc cttgttgacg acctgcggct gcagccgccc
    15121 gggccgctcc agccagtcct tgaggaacgc tttgcacttc ggatcggaca gccggaagaa
    15181 gtggtgctcc gaggtcttca gcaccggctt cgcgcccgag agcgtcgaat acgggttctt
    15241 caggtcggtg gcggcgtagg cggcgctgca gttctcgcag gcgtcgccgt attggtcctt
    15301 ggcgccgcag ttcgggcact cgcccttgat gtagcggtcg gcaaggaaca tgttctttac
    15361 cgggtcgtag aactgctcga cgggccggat gtcgatgaga ttggcgcgct tgagccgccg
    15421 gtagatgtcc tgcgacagcg cggtgttttc cggcgaatgc gtcgaatgcc agttgtcgaa
    15481 gccgatatga aagccctcca ggtactgcgc acggccacgc gcgatctcgg cgacgaacgc
    15541 gtccggggcc ttgccggcct tctccgccgc gagcatgatc ggcgcgccgt gcgcgtcgtc
    15601 ggcgcccacg aaatgcgcct catggccgcg catgcgctgg aagcgcaccc agatgtcggc
    15661 ctggatgtac tccatgatgt ggccgatgtg gaacgcgccg ttcgcatagg gcagcgcggt
    15721 ggtcacgaag aggcggcgtc gagacatgga gttacgcgaa aaagcgcaat tttatccgtg
    15781 ttttaggtac actaccgggc cccttcgtct attcccgcca tggccatctc cgaagcagac
    15841 gtgaaagccg cgctcggcgc gctcatcgat ccgaacaccg gcaaggattt cgtcagcacg
    15901 cgctcggtga aggcaatccg cgtcgccggc gcggacgtcg aggtcgacat cgagctcggc
    15961 tatcccgggc gcagtcagtt cgagcccatt cgccgcgagg tgattgcggc gctgaagaaa
    16021 tccggtgccg ccagcgccaa cgtgaaggtg gtggcgaaag tcgtaccgca ctccgtgcag
    16081 cgcggcgtga agctggtgcc ggggatcaag aacatcatcg ccgtggcctc cggcaagggc
    16141 ggcgtcggca agagcaccac cgcggtgaat ctcgccttgg cgctggccgc cgagggtgct
    16201 gcagtgggcg tgcttgacgc ggatatctac gggccgtcgc agccgacgat gctcggcatc
    16261 gctggccggc cggagtccaa ggacgggaaa tcgctcgagc cgatggtcgg gcacgggctg
    16321 caggcgatct cgatcggctt tctcatcgac gtcgatacgc ccatggtgtg gcgcgggccg
    16381 atggtgacgc aggcgctcga gcagttgctg aaggacacgc gctggcgcga gctcgactac
    16441 ctggtggtcg acctgccccc gggcactggc gatatccagc tcacgctcgc gcagaaagtg
    16501 ccggtgaccg gcgtggtgat cgtgaccacg ccgcaggaca tcgcgctcat cgatgcgcgc
    16561 aagggcctga agatgttcga gaaggtcggc atcccgatcc tcggcgtggt ggagaacatg
    16621 tcgctgcacg tgtgcccgaa atgcggtcac gagagccaca ttttcggcag tggcggcgcc
    16681 gcgcgcatgt gcaaggaata cggcatggag ctgctcggcc agctcccgct cgacgagtcg
    16741 atccgcgagc aggccgattc cggcaagccg accgtggtct ccgatcccga gggccgggcg
    16801 gccgagatct accggcgcat cgcgcggcgc tgcgcggtca agatcgcgga gtcgcagaaa
    16861 gacatgacct cgaagttccc caacatcgtc gtgcagaaca cctgagcggc gtgacgcccg
    16921 cggccgtcca tcagagccac aacttatagt aggctggaag cttagcagcg gtacctattg
    16981 tggtttctca gagccggcgc ccgcctcctc ccagtgcggc gccccgaaaa tcagtgcaca
    17041 aagcgcttga ctcgcgaggg gtcatcccta taatcgcccg aagttttccc tgatggctca
    17101 acgagctatg aaggaggtca tcatgacagg ccagaggacc gtgtacccgg tgtacgacgt
    17161 cgtcgttcgc gacattcctg agacctagat agcccctggg agaacattga tgggcgatcg
    17221 ccctggcagc ctcgcacgca accgacagca gaccgcaaac ccgcaagagc agctctttga
    17281 cacgcacccc gaggtcagcc tcgacttcga ggtcgtccgc caggccgcgc gccagaagta
    17341 cacgcggccg ttcctgatcc tcgacaccgc catcgtgcgc gaaaagctgc gccgcttccg
    17401 cgcggccatg ccgcgcgtgc ggccgcacta cgcagtcaag gccaaccccg accggcgcgt
    17461 gctgaaggtg ctggcgcagg aaggcgcggg cttcgagatc gcttccaccg ccgagctcga
    17521 ccaattgctc tcgctcgggg tgccggccgc cgaggtgttc ttctccaacc cgatgcgctc
    17581 gcgcgacgcg gacgcctacg cggtggcgaa gggcgtgcgc tggttcgtgg tcgatagcgt
    17641 cgatgagctc aagcgcatgc acgcggtgga cccgacggcg tgcctgtacc tgcgtatcgc
    17701 cacgccgaac attggcagcg actggccgct ctcgggcaag ttcggtgccg gcgccgcgga
    17761 ggcgcgcgaa atcatcgcca ccgcgtccaa gctgggtgcg gatctggggg gcgtcacctt
    17821 ccacgccggc tcgcagtgcc gcaacccgga gaactggcgc gtcggcatgg aaaaggcgcg
    17881 cacgctgttc gacgccatgg cgaaggccgg cctcaagccg cgcctgctgg acatcggcgg
    17941 cggcttcccc gtgcgccacg tgaagcccat tccgtcgatc gaagtgatcg gcgcagtggt
    18001 caacgaggcg ctacaggcgt ttcccgacga cgtgcaggtg atcgcagagc cgggccgcta
    18061 cctcgtgtcc gatgccggct acttcgtgtg ccgcgtgctg ggcacggcga tgcgcgccgg
    18121 caagcgctgg atgcactggg acgcgggcct gttcggcggc gtcatcgaga ccacggaagg
    18181 cctgaagtac aagatccgca ccgagcgctc gggcccggac atcgcgtgga acgtggcggg
    18241 gccgacctgc gactcggtcg atgtcgtgat gcgcgacgag ccgctgccct cagacctgca
    18301 ggagggcgat ttcatctaca tccgcaatgc gggcgcctat accaccgcct acgccagcgg
    18361 ctttaacggc tttccgctgc ccgaggtcag ggttttcgag tcgcgctcgc gctcgtagag
    18421 cacgaagtcg aagcgcaagc cgtcggcggc ggtgtgcggc ttgcgctggg tctcgcgcca
    18481 ggccgagcgg tcgtacggtg ggaagttcgt gtcgccgggg aactcgcgcc cgatctcggt
    18541 cagcaccatg gcgtcggcaa gcggcagcgc cgccgcgtaa agctcgccgc cgccgatgac
    18601 gaaggccaag ggttcgcccg cgcacagggc gagtgccgct tccagtgagc cggccacatg
    18661 cgcgcccggg gcgtcgaagc cgggggcgcg ggtcaccacg atgttctctc ggccaggcag
    18721 cggcttgccg agcgactccc aggtcttgcg ccccatgatc accgggtgtc cgagcgtcag
    18781 ttccttgaag tgccgcaggt cttcgggcag gcgccagggc agcttgcccc cggatccgat
    18841 gaccccgttg ctggccacgg ccgcgaccaa gtagacgcgc gtcccggcgg ggtgtgacat
    18901 aaggctcgtc ctgacgttgt tttacgtgag attatagggt tatggcacct caggagagtg
    18961 ctccgaggcc ggccctgcgc catccgcgct cctttctcgg attgctcgtt accggcttcc
    19021 tgctggtggc gctgccgctc gccggcgcgc tgctgtattc ggcctggagc acgcagcgcc
    19081 tcgccgagca gagtcgtcat gcggtgtaca acgcggcgca ggccgcgcgc ggcagccgat
    19141 cgctcgtcaa tcgcatcgct tccgtcgagc gcctcgcgca gcaggtggcg gtccttccgg
    19201 acgccggcct gctgcaggac tacgagcgcc tgcacgaagg cttccagctg ctcaccgccg
    19261 acctggcgcg cctgccgctc gacgtccagc agcttgcggc gctcgatcgc acggtggcgc
    19321 gcgagcaggt gctctacgag ctgctcgcgg cggcgccgca ggcgccgccc gacatcgcca
    19381 cggtgcgcgc gcgcgtcggc gagctcgctg aaagcgcgca cgaggtgctc gcgatcagcg
    19441 cgcgcgtcgc cgaccaggag gtggaacaac tgcgctcgag cgccgagatc gtgcagcgcc
    19501 gcctgctgaa tctcgtgctc gtcgccatcg cggtcgccct ggtcgcggcg ctcgcactca
    19561 cgcgcgtcat cgcgcggccg atcgccgagc tcgacgccgg catccgccag ctgggcggac
    19621 ccgatttctc cgccccgatc cgcgtcagtg ggccgcagga cctgcgcgac ctgggcgagc
    19681 ggctggactg gctgcgccag cggctcaccg acctggaagc gcagaagaac cgcttcctgc
    19741 gccatctgtc gcacgagctg aagacgccgc tcgcctcgct gcgcgagggc gcggagctgc
    19801 tcaacgacga ggtggccggg ccgctcgcgc cggcgcagcg cgcggtggtc ggcatcatgc
    19861 gcgacaacag cgtgaagctg cagcggctga tcgaagacct gctcgattac cagcgcgcgc
    19921 tgcacgcggc cgcctcgctc gaggtgagta cggtgcagct cgacgccctg gtgcgcgaag
    19981 tagccgacgc gcaccggctc tcggcgctcg ccaagggcca gaacgtggcg ctggagctgg
    20041 cgcccgccgt ggtcgaggcg gacgcggtga agctgcgctc gatcgtcgac aatcttttcg
    20101 gcaacgcggt gaagttcacg ccggcaggcg gtaccattac ggtgcgcgcg cacgaagccg
    20161 acggcaaggc ggtgatcgag gtgatcgact ctggtcccgg cgtgccgctg gtcgagcgcg
    20221 aggcagtgtt cgagtcgttc ttccgcgggc gcaacaaggc cggcggccgc gtcgccggca
    20281 gcgggctcgg cctggcgata gcccgcgagt atgtcgaggc gcacggcggg cgcattgcgc
    20341 tggtcgccga aggcgagggc gggcattttc gcgtcacgct gcccaagcgc ccggcgctgc
    20401 tggcgatgga ggaggcgaca tgagggtggc ggccctcctc tgcgccgctc tgcttgccgg
    20461 ctgtgcgctg ctgcggcccg acgcccgcga ggtcgtgcag gtgcgcgaga ttgtcggcgc
    20521 tgctgtaagc gcggcgcacg cgcctgcagc tgagcagcgc cgccagctcg tgcacgcgga
    20581 gcagacgtac gtggcggttc cggacgaggt gaaccgcgtg cggctcgccg cactgctcgc
    20641 gacgctgccg gcaccttggc gcgacgaagc gcgcgcggca agcctgctgg agccgctggc
    20701 gacgcgccga gccgacaccc cggtggcgca gctcgccggg atgctcgcgg cgaacgtggc
    20761 cgagcggcag cgcctcgcgc gtgaactgcg cgcggcggaa gcgcgcacgt ctgcggcgga
    20821 gtcgcgtgcg gcggcggcgg agtcgcgcgt ggcggcggcc gagtcgcgtg cggcggccgc
    20881 cgagcggcgc gaggaagcgg cggcggcgcg tgctggcgtc ctgcaacacc aggtagacgc
    20941 actgaaggca atcgagcgcg gtatcctcga gcgtgaggag cggcgcagga aacagcaaag
    21001 gtgaaaccat gagccacgaa gccccctcca tcctgctggt cgacgacgac cgcggcctgc
    21061 tgcaactgat ctccatgcgg ctgacggccg cgggctaccg cgtcacagcg gtggaagcgg
    21121 cggaggcggc gctcgccagc ctggccgtgc agcgcccgaa cctggtgatc accgacctgc
    21181 gcatgcaggg catggacggc ctgggcctgt tcgacgccat ccatcgacag gcgccctcgc
    21241 tccccgtggt gatactcacc gcgcacggca ccatccccga cgccgtggcg gcgacgcgcc
    21301 gcggcgtgtt cagctttctc accaagccgt tcgagcccaa ggtactgctg gaaacggtcg
    21361 ccgacgcgct gcggctatcc acagcgccga gcggcgaccg cgcgcactgg cgcagcgaga
    21421 tcatcacgcg ctcggcagcg atggaagcgc tcctcgggca ggcgcagcgg gtcgcggcgt
    21481 cggacgcgag cgtgtgcatt ttcggcgcga gcggaaccgg caaggagctg ctggcgcgcg
    21541 ccattcaccg cgccagccgg cgcgccgagg cgcccttcat tgcagtgaac tgcggcgcga
    21601 ttcccgaggg cctgctcgaa tcggagctct tcggccacaa gaggggtgcc ttcaccggcg
    21661 cggtgcagga ccgccgcggg ctgttccagg cttgcgaggg cgggaccctg tttctggacg
    21721 aagtggggga catgccgccc gcgctgcagg tgaagctgct gcgtgcgctt gaagatcgcg
    21781 tggtacggcc ggtcggcgcg aacgacgccg tgccgatcga cgtgcgcatc atctcggcca
    21841 cgcaccgcaa gctcgacgag cgtattgcca cgggcgagtt ccgcgaggat ctgttttacc
    21901 ggctgaacgt ggtgaagctc gagctgccgt cgcttgcgca gcggcgcgag gacatcccgc
    21961 tgctcggggg gcatttcctt gcgcgcctcg ccgagcgcta cggccgcccg cggctcggca
    22021 tctcgcccga ggcgatggag cagctggtca gcgccgcctg gcccggcaac gtgcgccagc
    22081 tgctcaacgt tatcgaacgc gcggtggcgc tcaccacgac ggagatgatc tcggcgtcgc
    22141 tggtgcggca ggccctggac gtcggcgcgg gctcgctcac gccgctcgac gaggcgcggc
    22201 gcgctttcga gcgcgagtac ctggtgcgca ttctcaagat caccggcggc aacgtcacgc
    22261 aagccgcacg gctggccggg cgcaaccgca ccgagttcta ccggctgctc gagcgccacg
    22321 cgctcgagcc aggcatgttc aagacgaagg tcgctgccga atcctgaccg gttgtcgccg
    22381 agcggcgaca gagaaagctg cgcgcagtca gtcccttgcc gtcggtgtga aatcgttcgt
    22441 cgctgccggc agacgtttgg gccacgtttg cgggcgcgta cttgttcacg caagggcgcc
    22501 aacccattga agaagatgat ttttcctgcg cgggcacgga gcttgcaagg atttgtgcga
    22561 gtccgatgga acacaccaag ggaaagttcg agattccgat cctggcacag gtcgccggcg
    22621 gctttgccat tagcgcgctg gtcctgatcg gcatcagcgg gtcgatctac aagctggtcg
    22681 cgccgcaggg ctgggtggtg caggcattcg gacacagttt tggcctgggc gcggcgctgc
    22741 ttgcggcctt gctcggcgct gccgcagtgg tgtgggcggt gcctggctat atgccggtgc
    22801 agcgcgctcg cctcgccgat gcggtggtgt atggctttgc ggcagcgggg ttgctgtacc
    22861 tgggccagat gctggctctc ggaggcttct aaagaaggaa cgcctcgacc ctgtcgcacc
    22921 tcctcccccg ggcccgtcct tcccccctac aggcgggccc ttttttttag accgcgatcg
    22981 gcgccctgat cgccgggtgc gggtcgtagt cctcgagggt gaagtcctcg tagcggaacc
    23041 cgaacaggtc gcgcaccgcc cggttgaggc gcatgcgcgg gaacgggcgc ggtgcgcgcc
    23101 cgagctgttc gcgcgcctgc tcgagatggt tcaggtacag atgcgcgtcg cccagcgtga
    23161 gtacgaactc gcccgggcgc aggtcggtga cctgcgccac catcagcgtg agcagcgcgt
    23221 aggacgcgat gttgaagggc acgccgagaa acaagtcggc gctgcgctga tacatctggc
    23281 acgaaagccg ccccccggcg acgtagaact ggaacagcgc gtggcagggc ggcagcgcca
    23341 tcttgtcgac gtcggccgga ttccaggcgc ttaccaggtg ccggcgcgaa tccgggcgcg
    23401 ttcgcaggct gtcgagcacc tggcggatct ggtcgatctc gcgcccgtcg gccgtgcgcc
    23461 agtggcgcca ctggtagccg tacacggggc cgagctcgcc gttctcgtcg gcccattcgt
    23521 cccagatcgt gacgccgcgc tcctgcagcc agcgcacgtt ggtgtcgccg cgcaggaacc
    23581 acagcagctc gacgatgatc gacttcaggt gcagcttctt cgtcgtgagc agcgggaagc
    23641 gctcctcgag cccaaagcgc atctggcgcc cgaacaccga gcgcgtgccg gtgccggtcc
    23701 ggtccgactt cgccgcgccg cgctccagca cttcggcgag gaggtccagg tactggcgtt
    23761 cgccgctgtc cgggctcata tggcgatcat cgcaaaaagc tcgtgcgcgc tctggaagcg
    23821 ttctgccggc gtcttggcga gcagccggtc cagtatgccc tgatagccgg ccagccgcgc
    23881 gggcaagcgc ggaatggggc catgcacgtg ctggtagacc aggcccgcag ggccctcgcc
    23941 ctcgtacagg cgccggccgc tcagcatctc atagaagatg acgccgagcg aatacaggtc
    24001 ggagcggtgg tcggcggacc ggccgaggca ctgctcgggg ctcatgtagc gcggggtggc
    24061 gatcaccatg ccgtggcggg tgagcgtggt cttcgccgac aggttcttcg ccagcccgaa
    24121 gtccaccagc accgggcggc cgttgtcgcg aaacaggatg ttgggcggct tcaggtcgcg
    24181 gtggatcacg ccgcgctcgt ggatcgcacc cagcgcgcgc gccacctgcg aggtgatgcg
    24241 cagtgccgtg agcgaggtca tgccctcgtg aatgcgcacc gcaagcgtgc cgccggcgag
    24301 gtactccatg gcgatgaacg ggtgctcgcc ggcaaagccc tgatcgtaga tgtgcgcgac
    24361 gtactcgttc tgcaggcccg cgatcagccg atactcgcgc acgaagcgct tgaagaaggt
    24421 ctcgtcggcg cgcagcttcg catccagcac cttgagcacc agctgcaggc cgtcgcgctc
    24481 gcgctcggcc aggtacacct gcgccatgcc gccttcgccg atcttgcgca agctgcggta
    24541 gccggggatg cgccgctcgt cgccctccag gggcgcaccg atgcgctgcg cgtcgagctt
    24601 gacggtacgc tcgaacggcg ccatcgggtt ggcggcctcg gtgcgccgcg cctcctgctc
    24661 gcgcaccgcg tcctcgatcg agcgcaccaa ccgctcgccg gtgaggtccg gcttgcgcag
    24721 gaaatccgcg gcaccggcct tcaagccctg gagcgccaga ttctcgccgc ccgattcggt
    24781 gagcaggatc accggcggcg cggccggatc gcgcagcatg cccgccatcc acgccagcgc
    24841 gtcgcttgcg gtgggctcca cgtacatgag gatcgcggcg tagctcgtca gcttgggcgc
    24901 ggcctccggg ctgccgcgct cgcgcggatc ccattcctcg attgtggcgt ccggccagcg
    24961 cgcgcgcagc atgccggcca ctgcgccgcg caggtcgcgg gatggatcca cgatgagaaa
    25021 tcgcatcaca gaaccgcgcg cagccaggcc gcgatatccg cgatctcctc cgcgcacacc
    25081 gcgtgcggca tcgggtattc gtgccaatcg accttgcagc cgagggcgtc gagcgcggcg
    25141 cgcgactgcc gggcacgctc gatcggaatg atgtcgtcga agcggccatg ggccatgaac
    25201 agcggcgcgc cgaggttggc cgcggcgtgc tccgttgcaa ccgtgccggc gagcggcagg
    25261 taggtcgaca gcgcgaggat gccggcgagg cgctcggcct gccgcagccc ggtgtgcagc
    25321 gcgatggcgc cgccctggga gaagccggcc agcacgatgc gcccggttgc cacgccgcgc
    25381 gcgcgctcgc gcgcgatcag cgcttcgagc tgggcctgcg aggcgcggat gccggccgcg
    25441 tcctcggccc cgccgccgag ctgcgcgatg tcgtaccagg cgcgcatgtg cattcccatg
    25501 ttgagggtca ccgggcgcac cggggcgtgc gggaagacga agcgcacgcg cagcgcgcga
    25561 ggcagttgca gctgcggcac taccggctcg aaatcgtggc cgtcggcgcc caggccgtgc
    25621 agccacagca ccgcggcgtc gggcgacggc gcgctctcga tctcgattgc ttcgagcagc
    25681 ggcgtcacgc cgggcgcggc cgctgcgccg actttggccg gaacgccttc accacggcct
    25741 cgtcggtctc gatgtagggg ccgccgatca ggtcgatgca gtagggcacc gcggcgaaca
    25801 cgccgtcgag cgtctccttg atcgccttgg gctggcccgg caggttgatg atcagcgcct
    25861 gccgccgcac caccgccacc tggcgcgaca ggatcgccgt cggcacgtac ttgaggctga
    25921 cggcgcgcat ctgctcgccg aagccgggca gcaccttgtc ggcgaccgcg agcgtcgctt
    25981 ccggcgtcac gtcgcgcggc gccgggccgg tcccgccggt ggtgagcacg aggtggcagc
    26041 ccctggcgtc aaccagctcc accagcgcgg tctcgatcac ggcccgctcg tcgggaatca
    26101 ggcgctcctc gtagcgcacc ccgggtgcga cgatcgcccg gccgagccag tccttcagcg
    26161 ccggaatgcc ctcgtccttg tagacgccgc ctgcggcgcg gtcgctcgcc gacaccacgc
    26221 cgacgaccag cacgctactc accggcggcc tcacgcagca cgcggaacag ctcacggaag
    26281 gcgcgcggcg ggcggcccgc ggcctgctcg cggcgcgcgt tgcgtatcag cgcgcgcagc
    26341 tgctgcaggt cgcccgacgg gtgcgcttgc gcgaactcgg tgagggcgtc gtcgtcgtcg
    26401 atcaggcgcg cgcgccaatg ctcgagccgc tgatgacgcg cgcgctcctc ggccgagccg
    26461 ccatgcaccg ccgcgagctg cgcgcgaatc ggcgcggcgt cgacgccgcg catcagcttg
    26521 ccgatgtact gcacctggcg ccggcgcgct tcgtggctat cgatgctgcg cgcctcatgc
    26581 actgcccgcg ccagctcggc gggcagcgtc atgcgctcaa ggtgcgcggc ggacagcgcg
    26641 accagctcgg cgccgagcgc ctgcaactcg tgcatctcct gcttcctgcg cgtcttgctg
    26701 acggtgtcgt cccgcatgcc gctgctatga tagcgcgcca tgaaaagcgg cggccgcttc
    26761 acctacacgc aggatcagct taaggcgctc gccgcgcagg tgctggcgca cgcccgccgc
    26821 ctcggcgcga gcgcctgcga atgcgacgtc tcggaaggtc agggactgtc ggtcacggtg
    26881 cgcaagggcg ccgtcgacac catcgagcac aaccgcgaca agagcatcgg cgtcaccgtc
    26941 tatctcggcg agcgccccgc ggcgcgccgc ggccacgcca gcacctcgga cttctcggcg
    27001 ccggcgctgg cgcagaccgt cgaagccgcg ctcgccatcg cgcgccacac cgcggtcgac
    27061 gactgcgccg ggctgccgga gccggaactg ctcgcgcgtg aaaccccgga cctcgacctc
    27121 taccatccct gggacatcgg caccgaggag gcgatcggca tcgcccggcg ctgtgaagcg
    27181 gcggggtttg cgctgtcgcc gcgcatccgc aactcggagg gcgcttcggt ctcggcgcag
    27241 cacgcgcagt tcgtcttcgc taacagccat ggcttcatgg caggcttccc gacctcgcgc
    27301 cattcgctgt acgtggcatt gatcgccgag gacgcgcgcg gcatgcagcg cgacgactgg
    27361 tacagcgccg cgcgcgtgcc gcaagcactc gccgacgccg aggcgctcgg ccgctacgcc
    27421 ggcgagcgtg cgctggcccg gctcggcgcg cggcgcatcg gcacgcgccg ggcgccggtg
    27481 ctgttcgagg cgccactcgc gacacagctg cttggcagct tcgtctcggc ggcgagcggg
    27541 ggcagcctgt accgcaaggc cacgttcctc gccgacagcc tcggcacggc ggttttcgcg
    27601 cccgggatca cgatcgagga gcggccgctc gagccgcgtg cgctcgcgag ctcgccgttc
    27661 gacgacgagg gcgtggcgac gcgcgcgcgc accgtggtgc gcgacggcgt gctcgaaggc
    27721 tacttcctcg gctgctacgc ggcgcgcaag ctcggcatga cgagcaccgg cagcgccgga
    27781 ggcaaccaca acctggtgct cgcctccaac gggccggatt tccgcggcat gcttgcgcgt
    27841 ctgcaccgcg gcctgctggt caccgagctg ctcggccacg gcgtcaacct cgtgaccggg
    27901 gactattcgc gcggcgccgc gggctactgg gtggagggcg gcgagatcgc ctatccggtc
    27961 gaagagctca cgatcgccgg gaacctgcgc gagatgttcc gcggcatcgc cagtgtgggc
    28021 ggcgacgtcg tggtgcgcgg ctcgcgccgc agcggctccg tgctggtgca gaacatgacc
    28081 attgcggggg agtaggccgg cgagaacctg ccgggttcca tacggtccaa taccggcacg
    28141 gaccttgcat tgcgaaaacc atgacttcaa cccttaggcc ggaacctaat agtgaacacc
    28201 cgatcgtcgc ctgggcgggt atccttgccg tggcagtcgc catcctcttg attctctggg
    28261 cgcgattcga ggtgcacggc acgaaagatc tggaagaagc gctgtctgag gacgcggcgc
    28321 ccgcctcggc agtcaagccc aagccgtcgc ccgaggccga caggtcgcac ggctcgaaag
    28381 tggaggaggc agtcgccgcg ggccgttggg ccgcggggtt ctcgccgtga aggaagcgca
    28441 gcccgctagg ccgtaaagtc gagccggtcg tcccgcagga ccccgtagat gcgaatcgag
    28501 gggcggttgt gcagctcgag cggcggcagc ggctccgcgt cgagctcaag ctcggccacc
    28561 gagatcacct tcatcagcgc atccgagagc accagctcgc ccgcgcgtgc gcgatgcacc
    28621 aggcgctcgg tgatgctgac gcagtcgccg aacaccacgg catttcgctg cgcgcccgga
    28681 ccggcttcgc cgtagaccgc ctcgccgcgg tgcaccccga gcgcgatcgc ggtgcgcagg
    28741 ccgtactcgc gctcccaggc ctgcacgatc gcgtcgaaat ctccgaacat ctgctgcgcc
    28801 gcgcgcaccg cggagcgcgc cagggcgttg atcgcggcgc cgtggaatac cgacatcaac
    28861 gagtcgttgt gcaccgccat gctgcggccg ccgttcttgg ccaccgcgcc cgcagcgcac
    28921 tcgaagaact ggtcggccag cccgagcacg cgctgcggct cgagcatttc ggacatgtgc
    28981 gtgaaattgc gcagctcggc gatcaggatc accgattcca cgcgctccgc ctggttctcg
    29041 ctctgcttgg ccacggctgc tacgttggca cccatgtcgg catctccccg caacgccacc
    29101 gggccatcgt aggcaagccc cctacggcac ggggaggaaa taattccgcc cggcgcatgc
    29161 accgggacag gcggcgccgc gcccccgatg ggcggcccgg ctcaggcaag gaacacgacc
    29221 atgccaacga cgagcaccac cagggcgccg gcggcgagaa aaatcagtga ggtgttggag
    29281 actttcggca ggtccgggtc gggcggcacc gggccggagg cgtgggcctc gtgcgcgtca
    29341 tgcgcgtccg tgtccgcgcc tgccgcgcca tgctgcgcgc cgaccttctc gttgaacgcg
    29401 gcgaagaaat ccttggagat cttcttcgcc gccgcatcga ccagccgcga gccgatctgg
    29461 gcgagcttcc cgccgacgct cgccttcacc tcgtacttga ggagcgtgcc gtcgccttcg
    29521 ggggcaagct gcacctgggc cgagcccttg ccgaagccgg cggcgccgcc ctgaccctcg
    29581 aaggcaatgg cgtaggagac cggcgggttg aggtccgaca gctgcagctt gcccttgaac
    29641 ttggcgctca ccggaccgac gcgcgccacc atcagcactt ggtactcgtt ctccgccacg
    29701 cgctcgatcg actcgcagcc cggcacgcag gccttgagga tctcggggtc gttgagcgcg
    29761 gcccaggtct ggtcctgggt ggccgggatg cgctcttcgc cgctcatttc catgcgcttg
    29821 cctccatggg tcgcgccagc ggccggctca gggtacgcgc cagctcgacc aggcttttca
    29881 gattatgcac cggcaggaac aagtccacgt gcggcagcat cgcgcgtacg cccgcgggct
    29941 tggcctcgaa gcgctcgtag cgaagcaggg gattcagcca cacgaggtaa tggctcgcct
    30001 tggcgaggcg ctccatttcc tcggcgaggc cctcgccggc ctcgcggtcc aggccgtcgg
    30061 ataccaggag cacgcaggca ttctgcccca gcacgcgccg cgcccagcgc cagttgaact
    30121 cgcgcaggca gacgccgatg cgcgttcctc ccgaccagtc cttgatggcg tcggcgacct
    30181 tcgccatggc gacgtcgacg tcgcggtgcc ggagctggcg cgtgacgttg gtgaggcgtg
    30241 tgccgaagac aaaggtgtgc acgcggtcgc ggtcgttggt aatggcgtgc aggaagtgca
    30301 ggaacatgcg cgcatacggg ttcatcgacc ccgagatgtc gcacagcacg accaggggcg
    30361 ggtgcacgac acgcgtcctg gcgcgcacca gcggaatgat gtcgccgcct tcgcgcaggc
    30421 tctcgcgcat cgtggcgcgc aggtgcacgc gcgtgccgcg gcggtgcggc gcgaggcggc
    30481 gcgtgcgcac catcggcagc ggcaggcgca gctcgcgcat catcttcttc gcctccgcca
    30541 gctccgccgc cgacatggtg tcgaagtcca tgcgctgcag catctcgcgc gaggaaaagg
    30601 tgagccgcgc gtcgagctcg atcttgcgct cctcgcgctc ttcctcgggc ttgttctgcc
    30661 caaggagcgc atcggtgagg cgctggcttt gctgctcctc gggctttggc gttcgccctt
    30721 cggtcttcgg cagcagcatc tgcatcatct tctcggtgag cttggggtcg cgccagaaca
    30781 ggcgaaacgc ctggtcgaag atcggccgct gctcctcgcg cgacagcagc accgaggaca
    30841 tggtccagta ccagtcctcg cgccgctcgg ccccggcgat tctcagcgcg cgcacggtgt
    30901 cgatgacgcg gtccggtccg acgcgcagcc ccgcggcgcg cagcaggcgc gcgaagtgca
    30961 tcacgttctc cgacagcttg ccgcggcctg ctgcggactc gagctgcggc gacatcctag
    31021 gccgcggcgg cggcgctctt cgcctcggcc accaggcgcg tcgcttcgtc gccggcgatc
    31081 ttctcgatgt cgtcctggta cttgaggagc acgccgagcg tgttgttgac ggtctcggga
    31141 tccagggcga tgcggtccag cgccaccagg caattggccc agtcgatggt ctcggcgacg
    31201 ccgggcagct tgaacaggtc gacgccgcgc aagcgctgca cgaaggccac cacttcgcgc
    31261 gacagcgcct gcgccgcccc cggcgctttc ttcttcagga tctccagctc gcgccgcgca
    31321 tccgggtagt ccacccagtg gtagaagcag cggcgcttca ccgcgtcgtg gatctcgcgt
    31381 gtgcggttgg acgtgatgac gacgatgggc ggctcggctg cgcgcagggt gccgatctcc
    31441 ggcaccgtga tctgccagtc cgagagcacc tccagcaggt acgcctcgaa cggctcgtcg
    31501 gttcggtcga gctcgtcgat gagcagcacc ggcgccgcgc ctggctttcc ttccagcgcc
    31561 agcgcgagtg gccgcttgat gagaaagcgc gcatcgaaga tgtcgtgcga catcttatcc
    31621 ttggatttct cgcccgcggc ctcggcgagc cggatctcga tcatctgccg ggggtagttc
    31681 cattcgtagg cggcctgcgc gatgtcgagg ccctcgtagc actgcaggcg gatcagctcg
    31741 cggccgagcg actgcgccac caccttggcg atctcggtct tgccggtgcc ggcctcgccc
    31801 tcgagaaaca gcggccgctg catggcgagc gaaaggtaca gcgcggtggc gaggctgcgg
    31861 tcggccacgt agtcggccgc ggcgagcagc gtctgggtct ggtcaatcga tgcggggagc
    31921 ggttgcggca tgcgccattt tagcgcgggg gcgcgcgcgg gcgcagccgg acggagagca
    31981 gccacagcag gttcaccagc acccagacga tgatgccgcc aacgaatgcg aacacggcga
    32041 gcccgagcgc gagcggcttg ccgatcgcgc cgaagcgctc gccccaggtt ttcggatcat
    32101 cgattggcgc ccgcgcctgc tcttcgacgt gcgcctgctc gacggggtca ggcacttgtc
    32161 ccagcagcgc ggtgccggtg cggtgcgcga gcacgaagat cgggccgtag gtgagcgggt
    32221 tggacaccag cgtggatacg gccgccaccg gcaggttggc gcgcagcagg agcgcgacga
    32281 gcgcggaggc gagaatctgc gccaccggga tcatgaagcc gaagaacacg ccgagcgccg
    32341 cgcccagcgc gacgctgcgg cggtcgaaat gccaaagccc gggacggtcg agcagcggcc
    32401 cgagccagcg caacgtgcga tgctcgcgca acttctcccg cgagggtagc cagcggtgca
    32461 gcgcgctact tcagcgccgc ctcgaccgcg cgcttggcca tcaccgtgat caggtgcgcg
    32521 cggtactcgg cggacgcatg caggtcgctg ttcagaccgt cggccttcac cttgaggttc
    32581 gccaccgaat cgggcgcgaa cttcgccgtc agcgccttct ccatctccgc gaggcgcagc
    32641 gcgcaggggc ccgctccggt gaccgccacg cgcaccccgt tgcccatgtc ggcgacgaac
    32701 acgccgacga tggcgaagcg cgacgcaggg ttcttgaact tggcgtacgc ggcgcgcttg
    32761 gggatcggga agctgaccga ggtgatgacc tcgccgggct tgagcgcggt ctcgtacagg
    32821 cccttgaaga acttgtcggc ggcgtgcgtg cccttggtgg tggtgatggt ggcggcgagc
    32881 ccgagcaccg cggccgggta gtcggccgcc gggtcgctgt tggcgagcgc gccgccgagc
    32941 gtgcccatgt tcctcacctg gcggtcgccg atcatgccgg cgagcgccgc gagcgcgggg
    33001 atcgccttct tcacctcggc ggagcccgcg acttcggcgt ggcgcgccat ggcgccgatg
    33061 atgacggtct tgccgtcgac cttgatgccg gcaagatcct tgatcatgcc gagatcgacg
    33121 atgtccgtag gcgcggcgag ccgcagcttc atcgcctgca ccagcgactg cccgccggcg
    33181 aggagcttcg cgtccgcgct ctttgccgca agcgcggcgg cgtcgttcac attggcggga
    33241 cggtggtagt tgaatgcgtg catcgtgatc tcctcaggcc gctttcttct gttgcagggt
    33301 gcgccagacg gtctgcggcg tcgccggcat ggcgatgtcc ttgacgccga gcgcgtcggt
    33361 gatggcgttg atcagcgccg cgggcgcacc gatcgcgccg gcttcgccgc agcccttcac
    33421 gccgagcggg ttgtgcgtgc agggtgtctc gcgcgtgtcc acctggaacg aaggcacgtc
    33481 atcggcgcgc gggatgcagt agtccatgaa cgagccggta accagctgcc ccgtcgagtc
    33541 gtacttcgca ccttccgtca acgcctggcc gatgccctga acgagcccgc cgtgcacctg
    33601 gccctcgacg atcatcgggt tgatgatctt gccaaagtcg tccaccgcgc tccagcgctc
    33661 gatcctcacc acgccggtgt ccgggtcgac ttcgacctcg cacacgtaag tgccggcggg
    33721 gaaggtgaag ttggacggat cgtagaacgc gttctcgttc agtccgggct cgagcttgtc
    33781 gtgcgggtag ttgtgcggca cgtaagcggc gaacgccacc tcgccgaaca tcttctgctt
    33841 gtcggtgccc gagaccttga atacgccgtt ggcgtactcg atgtcgcctt cggcggcctc
    33901 cagcaggtgc gcggcgatct tcttgccctt ggcgatgatc ttgtcgagcg ccttcacgat
    33961 cgccgtgccg cccacggcga tcgagcgcga gccgtaggtg cccatgccga agaggatctt
    34021 ggaggtgtcg ccgtgcacga tgtcgacgtt ctcgatcggc aggccgaggc ggtcggcgac
    34081 cacctgggcg aaggtggtct cgtggccctg gccgtgcgag tgcgagccgg tgaacaccgt
    34141 caccgagccg gtcgggtgca cgcgcacttc gcccgcttcg aacaggcccg cgcgcgcgcc
    34201 gagcgacccg gcgaccgccg agggcgcgat accgcaggct tcgatgtagg ccgcgtagcc
    34261 caggccgcgc agcttgcctt tagcctcgga ggccttcttg cgcgcggcgt agcccgccac
    34321 gtcggcgagc ttctgcgccg cgtccatcgt cgcgtcgtaa ttgccgatgt cgtactgcag
    34381 cgccaccggc gtctggtagg ggaactggcg gatgaagttc ttgcgccgga tctcggcggg
    34441 gtcgatcttc atctcgcgcg ccgcggtctc caccagccgc tcgaccacgt aggccgcttc
    34501 ggggcggccg gcgccgcgat aggcgtccac cggcgcggtg ttggtgaaca ccgccgtcac
    34561 ctcacaatgg atgagcggcg tcgtgtactg tcccgagagc agcgtcgcat aaaggatcgt
    34621 cggcacgcag gaggcgaacg tcgacaggta agcgccgata ttcgccgtcg tcttgacccg
    34681 catggcgagg aactttccgt ccttgtcgag cgcaagttcg gcgaccgtgt ggtggtcgcg
    34741 tccgtgcgcg tccgagacgt acgattccga gcgatcggcg gtccacttga ccgggcgccc
    34801 gagctgcctg gacgcccaag taacgaccac gtcctcggcg tacaggtaga tcttggagcc
    34861 gaaaccgccg ccgacgtcgc ccgccaccac gcgtaccttg tgctcgggca ggcccatgac
    34921 gaaggcggtc atcagaaggc gctcgacgtg cgggttctgg ttggcgacgt acagcgtgta
    34981 gctgtcgtcc gcgcggctgt aggagccgtt ggccgcgcgc ggctcgatcg cgttcgggat
    35041 cagccggttg ttgacgaaat cgagcttggt aacgtgcgcc gcattggcga aggccgcgtc
    35101 caccgccgcc ttgtcgccca acgcccacac gtagcaggtg ttattcggcg cgatctcgtg
    35161 cagctgcggc gcgcccttgg cgcgcgccag gcgcacgtcg gccaccgcgg gcagcacttc
    35221 gtaatccacc tgcaccagct cggcggcatc ctgcgcctgg tcctgggtct cggcgatcac
    35281 cacggcgacc tgctcgccga catggtgcac cttgccctgc gcgagcaccg ggtgcggcgg
    35341 ctccttcatc ggctggccgt tgacgtcggt gatcagccag ccgcagggca ggccgccgag
    35401 cttggcggcg gcgatgtccg cgcccgtgaa tacgtcgagc acgcccggcg cggctttcgc
    35461 cttggccgta tttaccgact tgatcttggc gtggccgtgc ggcgagcgca cgaacgcggc
    35521 gtagctctgg cgcggcagcg tgacgtcgtc cgtgaactgg ccggcgccgg tgagaaagcg
    35581 gtaatcctct ttgcgctcga cgcgagcgcc gatcaggggt gcacccatgg gcgcctcctt
    35641 acttggcagc cgtcatggcc ttggacccgg ccatgatgga tttcacgatg ttgtgatagc
    35701 ccgtacagcg gcacagattg ccttccagcg cctcgcgcac gtcctgctcg ctcgcgttcg
    35761 ggtggcgctt ggcgaagtcc acggcgttca tcaccacgcc cggggtgcag aagccgcact
    35821 gcagaccgtg gtgctccttg aacgccgcct gcatcgggtg cagcgtgccg tcggccttgg
    35881 ccaggccctc gatagtgagg atgtccgcgc cctcggcctg catgacgagc atcgagcagg
    35941 acttcaccgc cttgccgttc atttgcacgg tgcacgcgcc gcactgcgcc gtgtcgcagc
    36001 ccacgtgcgt gcccgtgagg cgcagctgct cgcggatgaa ggtgaccaga agcgtgcgcg
    36061 cctcgacctc cccggaaacg gccttgccgt tgactttcat ggataccttg atagccatgc
    36121 ggcctccttc ggatggtggt gtctggcagt actggcggga cggccatcct agccaagcgc
    36181 gccccgtgga tcaatgttgc gccgcaatag cggccgcgga gtaagatctc cggcatggac
    36241 tccgtcgata tcgaggtgct gcgcagcgcc gaagcctggc gcaagtccgg acacggcgtg
    36301 acgctcggca ccatcgtcaa gacctggggc tcggcgccgc ggccggtggg cgcgctggtg
    36361 gcgatccgcg atgacggcca ggtggcgggc tcggtgtcgg gcggctgcgt cgaggacgac
    36421 ctgatcgaga aagtgcgcga gaaggcgctg gcgaaggacg cgccgcagct cgtcacctat
    36481 ggcgtgacca acgaggaggc aacgcgctgg ggcctgccct gcggcggcac cctgcaggtg
    36541 gtgctcgagc cggtgagcga ggcgagcggg ctcggcggcc tgctcgacac gatctcgcag
    36601 cagcagctgg tcaggcgccg gctggacatg gattccggcc gcgtgacggt cgaaccgggg
    36661 cgctggagcg acgcgctgga attcgacggc aaggtgctga gcacggtgca tgggccgcgc
    36721 tggcggcttt tgctgatcgg cgcgggccag ctcacgcact acctcgccga gatggcgaag
    36781 atgctcgatt accacgtgag cgtcgtcgag ccgcgcgagg agtacgccgc gggatggaac
    36841 gtgccggggg tgaagctcga ccggcgcatg cccgatgacg tcatcgccga atcgaacctc
    36901 gacggccaca gcgcggtggt ggcgctcacg cacgatccca agatcgacga cctggcgctg
    36961 atggaagcgc tgaagtcggc ggcgttctat gtcggcgcca tcggctccaa gaagaacaac
    37021 gacgcgcgcc gcaagcggct gctggagttc gacctgtcgc aggccgagat cgcgcgcctg
    37081 cgcgggccgg tgggcctgta catcggatcg aagacgcccc ccgagattgc ggtggcgatc
    37141 atggccgaga tgaccgcggt gcgccacggc gttaccgatc ccagctgggc ggcgaagccg
    37201 cagccgcgct tcgatccctc cgcgtgcgag gtcaagactt gaagctcgtc ggcctgctgc
    37261 tcgcggcagg ctcggctacg cgcttcggtt ccgacaagct gcagcatccg cttccgcacg
    37321 gcgtctcgat cgccgtgcag gccgcgcgcc acctgaaggc cgaagtgccc cgcgtcatcg
    37381 ccgctgtgcg gccggacgga ttggcgacgg cgcgcgcgct tgccgccgaa ggctgcgaag
    37441 tggtcgtgtg cgagcgcgcc gccgacggca tgggcgcgag cctcgcctgc gcggcgcgcg
    37501 cggcgggcgc agtcgacggc tacctggtcg cgctcgccga catgccgttc gtgcgccgct
    37561 ccaccatcgc cgcggtgcgc gaggccctgg agcgcggcgc gctgctggcg gcgccgtact
    37621 ggcgcgcccg gcgcggccat ccggtggctt tcgccgggcg gctgcgcggc gagctcgagg
    37681 cgctcgccgg cgacgaaggc gcgcgtcggg tgctggacgc gcacgccgca gcgctggtga
    37741 aggtgccggt gggcgatccc ggcgccgtgc gcgacatcga ccagccctcc gacctggcgc
    37801 cgccgctcgc ggtgtaggcg cgcggctttc cgggtagact cgccacaggg gatcttgagg
    37861 cacagcgcag cgtggaaacc gcccagcgcg cgatactgtt cgcggacgtc accgacagca
    37921 cgcgcatcta cgagtc
//
LOCUS       DBNHKE010000228        11163 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1472451, whole genome shotgun sequence.
ACCESSION   DBNHKE010000228 DBNHKE010000000
VERSION     DBNHKE010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1472451"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="murG"
                     /locus_tag="AC16O9_15565"
     CDS             complement(<1..1008)
                     /gene="murG"
                     /locus_tag="AC16O9_15565"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR6518073.1"
                     /translation="MRTALIMAGGTGGHVFPGLAVADEMRAAGWNVVWMGARSGMEAR
                     LVPAHGYRMTYVRAAALRGKGLLAALLLPANLLVGFVQAARAIFRLRPDVVLGMGGYV
                     AFPGGMMASLLARPLAVHEQNAIAGLTNRVLAAVADKAMTAFPEALKGAEWTGNPVRR
                     EIAAMAPPEARFAGRDGPLRLLVVGGSLGAQALNDCVPRALALLEPRPRVVHQSGEKH
                     LEALRANYRAAGVQGELVAFIDDMARRYAEADLVICRAGAITVAELSAGGVASLLVPF
                     PHAVDDHQSANARFLAEKAAALLLPQAELTPHKLAATLRALDRPRLLEMARRARGLGK
                     PD"
     gene            complement(1005..2168)
                     /gene="ftsW"
                     /locus_tag="AC16O9_15570"
     CDS             complement(1005..2168)
                     /gene="ftsW"
                     /locus_tag="AC16O9_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR6518074.1"
                     /translation="MSATYVFAKPAAAPEYDRSLAWAALLLMAIGLVMVYSASIATAE
                     ASRYTGANPAYFLLRHAVFLGAALAGAIVVFLLPVRFWQKCAPLAFLAGLALLALVLV
                     PGIGREVNGARRWLDLGAITVQPSELVKLAAVLYAADYTVRKHAVLKSFRRGLLPMLA
                     VMMFVSWLLLREPDFGALVVIAVTAFGILFLGGMNGRHFAALVAMLALGFALLVLTSE
                     YRMQRIFGFMDPWTDPFGRGYQLSHALIAFGRGEFLGVGLGASVEKLFYLPEAHTDFL
                     LAVIAEELGFLGVAAVIGLFTWVVMRAFAIGRQAALRERHFAALAAQGIGIWIGFQAL
                     INMGVNMGLLPTKGLTLPLMSFGGTGLLANSVALALLLRIDWENRQLARGMRA"
     gene            complement(2172..3503)
                     /gene="murD"
                     /locus_tag="AC16O9_15575"
     CDS             complement(2172..3503)
                     /gene="murD"
                     /locus_tag="AC16O9_15575"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR6518075.1"
                     /translation="MRLAGRAVLVLGLGDTGLAIARWVEGQGARARVADTRAAPPRAA
                     DFAGELRSGPFTPALLEGIELLCVSPGLSLQEDVVRAALARGIPVVGEIELFAWHVRA
                     HNHTRVLAITGTNGKTTVTALTGHLLRTAGVDVEVAGNIGPSALAALAARARLPAAWV
                     LELSSYQLETTWSLEPEAAAMLNVSEDHLDRYAGIDDYAAAKARIFMGQGVQVLNRDN
                     WRSLGMGLPNRRRVTFGLDAPASEEDFGIVDGALHEGSHGFARIAQLPIHGLHNVANA
                     LAACALARALDVPRERLAAGLASFRGLPHRLQRLRERRGVDWYDDSKGTNVGAALAAL
                     RGLGRPAVLIAGGDGKGQDFTPLAPAVSAHARAVLLIGRDAPLIERALAAAGVPLERC
                     ATLEDAVARAAALAREGDAVLLSPACASFDMFRDYRHRGEAFAAAVAALED"
     gene            complement(3554..4645)
                     /gene="mraY"
                     /locus_tag="AC16O9_15580"
     CDS             complement(3554..4645)
                     /gene="mraY"
                     /locus_tag="AC16O9_15580"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR6518076.1"
                     /translation="MLLEFAQWLARDVRLFNVFNYITLRAVLACLTALAISLILGPLV
                     IRKLTAYKIGQSVRDDGPKTHLTKAGTPTMGGALILLSIGITTLLWGDLQNRYLWVVL
                     LVTLGFGAIGWIDDYRKVVHRNPKGLSARAKLFWQSAIGLAAAAYLASIAQGNPALNE
                     LIVPFFKFVAYPLGPIGFIILTYFVIVGTSNAVNLTDGLDGLAIMPTVMIGAALGIFA
                     YVAGHSVFSKYLGLPYIAGAGELTVFCGALAGAGLGFLWFNAYPAEVFMGDVGALALG
                     AALGTMAVIVRQEIVLFIMGGVFVVETLSVMLQVIYFKATGGRRIFRMAPLHHHYELE
                     GWKESQVVVRFWIITMMLVLFGLSTLKLR"
     gene            complement(4645..5997)
                     /locus_tag="AC16O9_15585"
     CDS             complement(4645..5997)
                     /locus_tag="AC16O9_15585"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR6518077.1"
                     /translation="MMSLAETAAATQGRLRGTDARYTAVSTDSRGVGAGELFVAIRGE
                     RFDGHDFIAAAKARGAAAALVDARFGGEAPLPLVVVDDTRRALGRLGRHWRSRFTPVV
                     IGIAGANGKTTTKEMLASILRAHAGEGAVLATKGNLNTDIGVPLTLLGLTGAHRYCAV
                     ELGMNHPGEIEWLAAIAQPTIGLVTNAQREHMEFMGSVEASAAENAHVYRALPATGIA
                     VLNADDACHGIFVEAAGARRVVEFGLAPKAAVGGGYALRPLASDIVVRTTDGEARARL
                     AIAGLHNVRNALAAAACAHAAGVPVATIGRGLEAFRPYSGRMQLKRTQSGATLIDDSY
                     NANPDSVRAAIDVLAACQAPTVLVLGDMGEVGTQGPQFHREIGAYARERGVGALFALG
                     EASREAVAAFGKGGRHFASPAHLQAALPAAATVLVKGSRFMRMERVVAALTGEAEGSH
                     "
     gene            complement(5994..7445)
                     /locus_tag="AC16O9_15590"
     CDS             complement(5994..7445)
                     /locus_tag="AC16O9_15590"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR6518078.1"
                     /translation="MDPLGQLARQGAMIERLAADSRRCAPGVAFFAYPGERADGRRFI
                     PQAVAQGAAGVLWEAEGFAWNPAWRVPNAGVPDLRRCAGALAHEFYGRPSAALWTCGV
                     TGTNGKTTCTQWLAAALTGAGAKCGVIGTLGTGFPGQLEHARNTTPDALELHGTLADM
                     RRQGAVAVTMEVSSHGLQQGRVGGVAFDCALFTNLSHDHLDYHGTMDAYAAAKARLFD
                     TEGLGCAVLNLDDVFGAQLAQRMAARGVRTIGTSLVSAPATGEFIVASVREGAPLRIA
                     SSWGEAEVMLDALGRFNVSNALGVLGALIARGMAFGDAVRCLERLPDVPGRMQRLGGK
                     GAPLVVVDYAHTPDALEQVLRALRPVAAARGGRLVAVFGAGGERDRAKRAPMGAAASR
                     LADRVVLTSDNPRGEDPLAILREIEAGVDAPHTVEPNRMHAIHAAIDAAALADVVLLA
                     GKGHEATQEIAGQRLPFSDAEVARAALARRGKA"
     gene            complement(7453..9177)
                     /locus_tag="AC16O9_15595"
     CDS             complement(7453..9177)
                     /locus_tag="AC16O9_15595"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR6518079.1"
                     /translation="MSAAAPALVRLPVWRARFVMAGLVAAFLVLAARSLYLQAMQTEF
                     LQEKGEARYSRVLDVPGTRGRIVDRNGEALAISTPVQSVWAIPGDVQASPAQLKRLAA
                     HLGLEARELRRRLEGSSRDFVYLKRQIAPEAADKVAQLGLEGIYQHREYRRYYPGGEV
                     TAHVVGFTGVDEAGQEGVELAFESRLGGTPGNRRVIKDRRGRVVEDVESIRAAQDGRD
                     LQLSIDGKIQSLAYAALKGAVAAHKAKAGALVAIDVRTGEVLALANVPSFNPNNRARL
                     SGAQIRNRAVTDVFEPGSTLKPFTVALALESGRVSPSTLVHIGNGGLRIANYTIQDPH
                     PETDLSVAQVIQKSSNVGAARIALALRREDMWSLFERAGFGVAPHIGFPGEASGRLRA
                     AESWRPIEQATMAYGHGISVSLMQLARAYTIFARDGELVPATLLKAQAPAPGTRVVSA
                     QTARQVRAMLELAVQPGGTAPRARIMGWRVAGKTGTAHKQENGGYAVDRYIASFVGLA
                     PASQPRLVVAVMIDEPANGQYFGGLVAAPVFAEVARGALRALGVPYDAPLEPVPLPGA
                     GDEAREST"
     gene            complement(9174..9446)
                     /gene="ftsL"
                     /locus_tag="AC16O9_15600"
     CDS             complement(9174..9446)
                     /gene="ftsL"
                     /locus_tag="AC16O9_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR6518080.1"
                     /translation="MAKLNLLLLLVLVVCALGLVTSQHKARRLFSELERAQARARELD
                     VEYGKLQLEASTWALHTRIERVATGTLGMRTPDARQVRVVAVAGGK"
     gene            complement(9434..10417)
                     /gene="rsmH"
                     /locus_tag="AC16O9_15605"
     CDS             complement(9434..10417)
                     /gene="rsmH"
                     /locus_tag="AC16O9_15605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6518081.1"
                     /translation="MSAAPGTHRPVLAAEALDALAIRADGVYVDGTFGRGGHSRLILE
                     RLGPRGRLIALDRDPQAVSAARAIADARFSITHASFGALAQALEAQGAALVQGMLFDL
                     GVSSPQLDDPQRGFSFRADGPLDMRMDPSQGASAADWLAAASESELREAIGGYGEERF
                     AKQIAAAIVAARRHAPLRTTRELADLVGAAVRTREPGQDPATRTFQALRILVNRELEE
                     VSLMLPQALARLAPEGRLAVIAFHSLEDRIVKRFLQACARPALPRDLPLRASELPQAL
                     LRIVGKPVRPGRAETAANPRARSAVLRVAERTAAPYDPSLIDRIEPDAWRS"
     gene            complement(10427..10876)
                     /gene="mraZ"
                     /locus_tag="AC16O9_15610"
     CDS             complement(10427..10876)
                     /gene="mraZ"
                     /locus_tag="AC16O9_15610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6518082.1"
                     /translation="MGGTFHGATVVTLDAKGRLAIPTRHRAALLADARHLVLTAHPEG
                     CVLLYPPAAWEPVRTQAQSFPSFHPQASWWKRLLLGFEEHVQPDGSGRILLSGALRQH
                     ARLERDAMLVGQGHYFELWDSARWDDKLAHALSGPPSAPPGMEDFSL"
BASE COUNT         1659 a         4062 c         3956 g         1486 t
ORIGIN      
        1 atcgggcttg cccagaccgc gcgcgcgccg tgccatctcc agcaggcggg gccggtcgag
       61 cgcgcgcagc gtcgccgcga gcttgtgtgg cgtgagctcg gcctgcggca agagcagcgc
      121 cgcggctttc tccgcgagaa agcgcgcgtt ggcgctctgg tgatcgtcga cggcgtgcgg
      181 gaaaggcacc agcaggctcg ccacgccgcc cgccgagagc tcggcgacgg tgatcgcccc
      241 ggcgcggcag atgacgaggt cggcctcggc gtagcgccgc gccatgtcgt cgatgaacgc
      301 cacgagttcc ccttgcacgc cggcggcgcg gtagtttgcg cgcagcgcct ccagatgctt
      361 ctcgcccgac tggtgtacca ccctgggtcg cggctcgagc agcgccagcg cccgcggcac
      421 acagtcgttc agcgcctgcg cgccgaggct gccccccacc accaggagcc ggagcggccc
      481 gtcgcgcccg gcgaagcgcg cctcgggcgg cgccatcgcg gcgatttctc gccgcaccgg
      541 gttgccggtc cactcggcgc ccttgagcgc ctcgggaaac gccgtcatcg ccttgtcggc
      601 gaccgcggcg agcacgcggt tggtgagccc ggcaatcgcg ttctgctcgt gcacggccag
      661 cggccgcgcg aggagcgacg ccatcatgcc gccgggaaac gccacgtagc cccccatgcc
      721 cagaaccacg tccgggcgca ggcggaagat ggcgcgcgcc gcctgcacga agccgacgag
      781 caggttcgcc ggcaggagca gcgcggccag cagccccttg ccgcgcagcg cggccgcgcg
      841 cacgtacgtc atccggtagc cgtgcgccgg cacgaggcgc gcctccatgc cggacctggc
      901 ccccatccac accacgttcc agcccgcggc gcgcatttcg tcggcgaccg ccagccccgg
      961 gaacacgtgc ccgccggtgc cgccggccat gatgagcgcg gtcctcatgc gcgcatcccc
     1021 cgcgccagct gccggttctc ccagtcgata cgcagcagca gcgccagcgc gacgctgttg
     1081 gcgaggaggc ccgtgccgcc gaagctcatc agcggcagcg tgaggccctt ggtgggcagc
     1141 agccccatgt tcacgcccat gttgatgagc gcctggaacc cgatccagat gccgataccc
     1201 tgcgccgcaa gcgcggcgaa gtggcgctca cgcagcgccg cctgccggcc gatggcgaag
     1261 gcgcgcatca ccacccacgt aaacagcccg atcaccgccg cgacgccgag aaagccgagc
     1321 tcctcggcga tcaccgcgag gaggaagtcc gtgtgcgcct cgggcaggta aaagagcttc
     1381 tccacgctcg cgcccagccc gacgccgaga aattcgccgc ggccgaaggc gatcagcgcg
     1441 tgcgacagct ggtagccgcg gccgaacgga tcggtccacg ggtccatgaa gccgaagatg
     1501 cgctgcatgc ggtactcgga ggtcagcacg agcagcgcga agccgagcgc gagcatcgcc
     1561 accagcgcgg cgaagtgccg gccgttcatg ccgccgagga agagaatccc gaacgccgtc
     1621 actgcgatca ccaccagcgc gccgaagtcg ggctcgcgca gcagcagcca cgatacgaac
     1681 atcatcaccg cgagcatcgg cagcagcccg cggcggaagc tcttcagcac cgcgtgcttg
     1741 cgcaccgtgt aatcggccgc gtagagcacc gcggcgagct tcaccagctc cgagggctgc
     1801 accgtgatcg cgccgaggtc gagccagcgg cgcgcgccgt tcacctcgcg cccgatgccc
     1861 ggcaccagca ccagcgcgag gagcgcgagg ccggcgagga acgcgagcgg cgcgcatttc
     1921 tgccagaacc gcacgggcag caggaacacg acgatcgcac cggccagcgc cgcgcccagg
     1981 aacaccgcgt ggcgcagcag gaaataggcc gggttggcgc ccgtgtagcg gctcgcctcc
     2041 gcggtggcga tggaggccga gtacaccatt accaggccga tcgccatcag caggagcgcc
     2101 gcccaggcga gcgagcggtc gtactcgggg gcggccgcgg gcttggcgaa gacgtaggtg
     2161 gcgctcacgc gtcagtcctc cagcgccgcc accgccgcgg cgaacgcctc gccgcggtgc
     2221 cggtagtcgc gaaacatgtc gaagctggcg caggcgggcg agagcagcac cgcgtcgccc
     2281 tcgcgcgcga gtgccgccgc gcgcgccacg gcgtcctcga gcgtggcgca acgctcgagc
     2341 ggcaccccgg ccgcggcgag cgcgcgctcg atgagcggcg cgtcgcggcc gatgagcagc
     2401 acggcgcgcg cgtgcgcgct caccgcgggc gcaagcgggg tgaagtcctg ccccttgccg
     2461 tcgccgcccg cgatcagcac tgcgggccgc cccaggccgc gcagcgcggc gagcgccgcg
     2521 cccacgttgg tgcccttgga atcgtcgtac cagtccacgc cgcggcgctc gcgcagccgc
     2581 tgcagccggt gcggcagccc gcggaagctc gccaggccgg cggccagccg ctcgcgcggc
     2641 acgtcgagcg cacgcgccag cgcgcaggcg gcgagcgcgt tggcgacatt gtgcaggccg
     2701 tggatcggca gctgcgcaat gcgcgcgaaa ccgtgcgagc cctcgtgcag cgctccgtcc
     2761 acgatgccga agtcctcctc cgacgcgggc gcatcgagcc cgaaggtgac gcggcgccgg
     2821 ttgggcaggc ccatgccgag cgagcgccag ttgtcgcggt tcagcacctg cacgccctgc
     2881 cccatgaaga tgcgcgcctt tgctgccgca tagtcgtcga tgccggcgta gcggtcgagg
     2941 tgatcctcgc tgacgttgag catcgccgcc gcctccggct ccagcgacca ggtggtctcg
     3001 agctggtagc tggagagctc gagcacccac gccgcgggca gcctggcgcg cgcggcgagc
     3061 gcggcaagcg ccgagggccc gatattgccc gccacctcga catcgacgcc ggcagtgcgc
     3121 aacagatggc cggtgagcgc ggtgacggtg gtcttgccgt tggtcccggt gatcgcgagc
     3181 acgcgcgtgt ggttatgcgc gcgcacgtgc caggcgaaga gctcgatttc gccgaccacc
     3241 gggatgccgc gcgcgagcgc cgcccgcacg acgtcctcct gcagggacag cccggggctg
     3301 acgcacagca gctcaatgcc ctcgagcagc gccggcgtga acgggcccga ccgcagctct
     3361 cccgcgaagt ccgcggcgcg cggcggcgcc gcccgcgtat cggcgacgcg cgcgcgtgcg
     3421 ccctggccct cgacccagcg cgcgatcgcg aggccggtgt cgcccaggcc gagcaccagc
     3481 acggcgcgcc cggcaagccg catcggctcg ggccgcggca gcaggttgag gacgggctcg
     3541 cgcatcgaca tggtcaccgc agcttcagcg tcgagagccc gaacagcacg agcatcatgg
     3601 tgatgatcca gaagcgcacc accacctgcg attccttcca gccttcgagc tcgtagtggt
     3661 ggtgcagcgg cgccatgcgg aagatgcgcc gccccccggt cgccttgaag tagatgactt
     3721 gcagcatcac cgacagcgtc tcgacgacga acacgccgcc catgatgaac agcacgatct
     3781 cctggcgcac gatcaccgcc atcgtgccga gcgcggcgcc gagcgcgagc gcgcccacgt
     3841 cgcccatgaa cacctcggcg ggatacgcgt tgaaccagag aaagcccagt cccgcgccgg
     3901 cgagcgcgcc gcagaacacc gtgagctcgc ccgcgcccgc gatatagggc aggccgaggt
     3961 acttgctgaa cacgctgtgg ccggccacgt acgcgaagat gcccagcgcg gcgccgatca
     4021 tcaccgtcgg catgatggcg agtccgtcga ggccgtcggt caggttgacg gcgttcgacg
     4081 tgccgacaat gacaaagtac gtaaggatga tgaagccgat cggcccgagc gggtacgcga
     4141 cgaacttgaa gaacggcacg atgagctcgt tcagcgccgg attgccctgc gcgatgctgg
     4201 cgaggtaggc cgccgccgcg aggccgatcg ccgactgcca gaacagcttc gcgcgcgccg
     4261 acaggccctt cggattgcgg tgcaccacct tgcggtagtc gtcgatccag ccgatggcgc
     4321 cgaagccgag cgtcaccagc agcaccaccc acaggtagcg gttctgcagg tcgccccaga
     4381 gcagcgtggt gatgccgatc gacagcagga tcagcgcgcc gcccatggtc ggcgtgcccg
     4441 ccttggtgag gtgcgttttg ggcccgtcgt cgcgcaccga ctggccgatc ttgtaggcgg
     4501 tcagcttgcg gatcacgagc gggccgagaa tcagcgagat ggcgagcgcg gtgaggcagg
     4561 cgagcaccgc gcgcagcgtg atgtagttga agacgttgaa caggcgcacg tcgcgggcca
     4621 gccattgcgc gaattcgagc agcatcagtg actcccttcg gcttcgccgg tgagcgccgc
     4681 caccacgcgc tccatgcgca tgaagcgcga gcccttcacc agcacggtgg cggcggccgg
     4741 cagcgccgct tgcaggtgcg cgggcgaggc gaaatggcgc ccccccttgc cgaaggcggc
     4801 gaccgcctcg cggctcgcct cgcccagggc gaagagcgcg ccaacgccgc gctcgcgggc
     4861 gtaggcgccg atctcgcggt ggaactgcgg cccctgcgtg cccacctcgc ccatgtcgcc
     4921 gagcaccagc accgtgggcg cctggcaggc ggcgagcacg tcgatggccg cgcgcaccga
     4981 atcggggttg gcgttgtagc tgtcgtcgat gagcgtggcg ccgctttgcg tgcgcttgag
     5041 ctgcatgcgc cccgagtagg gcctgaaggc ctccaggccc cggccgatcg tcgccaccgg
     5101 gacgcccgcc gcgtgcgcgc aggctgcggc ggcgagcgca ttcctcacgt tgtgcaggcc
     5161 ggcgatggcg aggcgcgcgc gcgcctcgcc gtcggtcgtg cgcaccacga tgtcgctcgc
     5221 gaggggccgc agcgcgtagc cgccgccgac cgcggccttg ggcgcgaggc caaactcgac
     5281 gacgcggcgc gcaccggcgg cctcgacgaa gatgccgtgg cacgcatcgt cggcattcag
     5341 caccgcgatg cccgtggccg gcagggcgcg atagacgtgc gcgttctccg cggcgctcgc
     5401 ctcgacgctg cccatgaact ccatgtgctc gcgctgcgcg ttggtgacca ggccgatcgt
     5461 cggctgcgcg atcgcggcga gccactcgat ctcgccgggg tggttcatgc cgagctcgac
     5521 ggcgcagtag cgatgcgcgc cggtcaggcc gagcaatgtg agcggcacgc cgatatcggt
     5581 attgaggttg cccttcgtgg cgagcaccgc gccttcgccc gcgtgcgccc gcaggatcga
     5641 ggccagcatc tccttggtgg tggtcttgcc gttcgcgccg gcaatgccga tgaccaccgg
     5701 cgtaaaccgg ctgcgccaat ggcgccccag ccgccccagc gccctgcggg tgtcgtccac
     5761 cacgaccagc ggtagcggcg cctcgccgcc gaagcgcgcg tcgaccagcg ccgcggcggc
     5821 gccgcgcgcc tttgccgcag cgatgaagtc gtggccgtcg aaacgctcac cgcggatggc
     5881 gacgaacagc tcgcccgcgc cgacgccccg cgaatcggtc gagacggccg tgtagcgcgc
     5941 gtcggtcccg cgcagacggc cctgcgttgc ggcggcggtc tcggcgaggc tcatcatgcc
     6001 ttcccccgcc gtgcgagcgc ggcgcgcgcc acttcggcgt ccgagaatgg caggcgctgc
     6061 ccggcgatct cctgcgtcgc ctcgtggccc ttgcccgcga gcagcaccac gtcggcgagc
     6121 gcagccgcgt cgattgcggc gtggatggcg tgcatgcggt tcggctccac cgtgtgcggc
     6181 gcgtcgacgc cggcctcgat ttcgcgcagg atcgcgagcg gatcttctcc gcgcgggttg
     6241 tcggaggtga gcacgacgcg atcggcaagc cgcgaggccg cggcgcccat cggcgcgcgc
     6301 ttggcgcggt cgcgctcgcc gccggcgccg aacaccgcca ccaggcgccc gccgcgcgcc
     6361 gccgcgaccg ggcgcagcgc gcgcagcacc tgctccagcg catcgggcgt gtgcgcgtag
     6421 tcgacgacca cgagcggcgc gcccttgccg ccgaggcgct gcatgcgtcc cggaacatcg
     6481 ggcaggcgct cgaggcagcg caccgcgtcg ccgaacgcca tgccgcgggc gatgagcgcg
     6541 ccgagcacgc cgagcgcatt cgagacgttg aagcggccga gcgcgtcgag catgacttcg
     6601 gcctcgcccc agctcgaggc gatgcgcagc ggcgcgccct cgcgcaccga ggcgacgatg
     6661 aactcgccgg ttgcgggcgc cgagaccagg ctggttccga tggtgcgcac gccgcgcgca
     6721 gccatccgct gcgcgagctg cgcgccgaac acgtcgtcga ggttgagtac cgcgcagccg
     6781 agcccctcgg tgtcgaacag gcgtgccttg gccgccgcgt acgcgtccat cgtgccgtgg
     6841 tagtcgagat ggtcgtgcga caggttggtg aacagcgcgc agtcgaaggc gaccccgccc
     6901 acgcgcccct gctgcaggcc gtgcgaggac acctccatcg tcaccgccac cgcgccctgc
     6961 cggcgcatgt cggccagcgt gccgtgcagc tcgagcgcgt ccggtgtggt gttgcgcgcg
     7021 tgctcgagct gcccgggaaa gccggtgccg agcgtgccga tcacgccgca cttcgcgccg
     7081 gcgcccgtga gcgcggccgc gagccactgc gtacaggtgg tcttgccgtt ggtgccggtg
     7141 acaccgcagg tccagagcgc cgccgacggc cggccgtaga actcgtgggc aagcgcgccc
     7201 gcgcaccgcc gcagatccgg cacgcccgcg ttgggcacgc gccatgccgg attccaggcg
     7261 aagccctcgg cctcccatag cacgccggcg gcgccttgcg cgaccgcctg cggaatgaag
     7321 cggcggccat cggcgcgctc gcccgggtag gcgaagaacg ccacgccggg cgcacagcgg
     7381 cggctgtccg cggcgagcct ttcgatcatc gccccctggc gcgcgagctg tccgagcggg
     7441 tccatgcgcg catcaggtgc tttcccgcgc ttcgtcgccc gcgcccggca gcggcacggg
     7501 ctcgagcggc gcgtcgtagg gcacgccgag cgcgcgcagc gcgccccgcg ccacttccgc
     7561 aaacacgggt gccgccacca ggccgccgaa gtactggcca ttggcgggct cgtcgatcat
     7621 taccgccacc accaggcgcg gctggcttgc tggggcgagg ccgacgaacg aggcgatgta
     7681 ccggtccacg gcgtagccgc cgttctcctg cttgtgcgcg gttccggtct tgccggcgac
     7741 gcgccagccc atgatgcgcg cgcgcggcgc ggtgccgccc ggctgcaccg cgagctcgag
     7801 catcgcgcgc acctggcgcg cggtttgcgc gctcaccacg cgtgtgcccg gcgccggcgc
     7861 ctgcgccttg agcagcgttg ccggcaccag ctcgccgtcg cgcgcgaaga tggtgtaggc
     7921 gcgcgccaac tgcatcaggc tcaccgagat gccgtggccg taggccatgg tcgcctgctc
     7981 gatcgggcgc cagctctcgg ctgcgcgcag cctgccgctc gcttcgccgg gaaagccgat
     8041 gtgcggcgcg acgccgaatc cggcgcgctc gaacaggctc cacatgtcct cccggcgcag
     8101 cgccagcgcg atgcgcgccg cgcccacgtt ggaggacttc tggatcacct gcgcgacgct
     8161 cagatcggtc tccgggtgcg ggtcctggat ggtgtagttg gcaatgcgca ggccgccgtt
     8221 gccgatgtgc accagcgtcg agggcgacac gcgccccgac tcgagcgcca gcgccaccgt
     8281 gaagggcttg agcgtggagc ccggctcgaa cacgtcggtg accgcacggt tgcggatctg
     8341 ggcgcccgaa aggcgcgcgc ggttgttcgg attgaacgag ggcacgttgg cgagcgccag
     8401 cacttccccc gtgcgcacgt cgatcgccac cagcgcgccc gcctttgcct tgtgcgcggc
     8461 gaccgcgccc ttcaaggcgg cgtaggcgag gctctggatc ttgccgtcga tcgagagctg
     8521 caggtcgcgg ccgtcctgcg ccgcgcggat cgactcgacg tcctcgacga cgcgaccgcg
     8581 gcggtccttg atgacgcggc ggttgcccgg ggtgccgccc aggcgcgact cgaaggcgag
     8641 ctccacgccc tcctgccccg cctcatcgac gccggtgaaa cccactacgt gagcagtcac
     8701 ttcgccgccg ggatagtagc ggcggtactc acggtgctgg tagataccct cgaggccgag
     8761 ctgcgccacc ttgtcggcgg cctccggcgc gatctggcgc ttcagataga cgaagtcgcg
     8821 cgagctgccc tcgagcctgc ggcgcagctc gcgcgcctcg agcccgaggt gcgcggcgag
     8881 ccgcttcagc tgcgcgggcg aagcctgtac gtccccgggg atggcccaca ccgactgcac
     8941 cggcgtgctg atggccagcg cctcgccgtt gcggtccacg atccgcccgc gcgtgcccgg
     9001 cacatccagc acccggctgt agcgcgcctc gcccttttcc tgcaggaact cggtctgcat
     9061 cgcctgcaga tacagcgagc gcgcggcgag cacgagaaag gcagccacca gcccggccat
     9121 cacgaagcgc gcccgccaca ccggcaggcg caccagcgcg ggcgcggcgg cgctcacttg
     9181 ccccccgcca cggcgacgac gcgcacctgg cgcgcgtccg gcgtgcgcat gccgagcgtg
     9241 ccggtggcca cgcgctcgat gcgcgtgtgc agcgcccagg tgctcgcctc gagctgcagc
     9301 ttgccgtact cgacgtcgag ctcgcgggcg cgcgcctgcg cacgctcgag ctcggagaag
     9361 agcctgcggg ccttgtgctg ggaggtaacg agcccgagcg cgcagacgac gagaaccagg
     9421 aggaggagga ggttcagctt cgccacgcgt cgggctcgat tcggtcgatc agggacgggt
     9481 catagggggc ggccgtgcgc tccgccacac gcagcacggc gctgcgcgcg cgcggattgg
     9541 ccgcggtttc ggcgcgcccc gggcgcaccg gcttgcccac gattctgagc aaggcttgtg
     9601 gcaactcgct ggcgcgcagc ggcaaatccc ggggcagcgc agggcgcgcg caagcttgca
     9661 ggaagcgttt cacgatgcgg tcttcgagcg agtgaaaggc gatcaccgcc agccgtccct
     9721 ccggggcgag gcgcgcgagc gcctgcggca gcatcagcga cacttcctca agctcccgat
     9781 tgacgagaat ccgtagagct tgaaaggtgc gcgtcgccgg atcctggccc ggctcccgtg
     9841 tgcggaccgc cgcacccacg agatcggcaa gttcgcgggt cgtgcggagg ggcgcgtgcc
     9901 ggcgagcagc aacaatcgcc gctgcaatct gtttagcaaa ccgttcttcc ccatatcccc
     9961 cgatggcctc cctgagttcg gattcgcttg ccgccgcgag ccaatccgcg gcgctcgcgc
    10021 cctgcgacgg atccatgcgc atgtcgagcg gcccgtcggc gcggaaggag aacccgcgct
    10081 gcggatcgtc gagctgcggc gaagacacgc cgaggtcgaa cagcatcccc tgcacgagcg
    10141 ccgcaccttg tgcctccagt gcttgcgcga gcgcgccgaa agacgcgtgc gtgatggaaa
    10201 aacgcgcatc ggcgatggcg cgcgccgccg acactgcctg cgggtcgcgg tccagcgcga
    10261 tgagccgccc gcgcgggccg agccgctcga ggatcagccg gctgtgcccg ccgcgcccga
    10321 aggtgccgtc cacgtagacg ccatcggcgc ggatcgccag cgcatcgagc gcctcggcgg
    10381 cgaggacggg gcgatgcgtg ccgggcgcag cgctcatcgc ccgcgctcac agcgagaaat
    10441 cctccatgcc ggggggcgcg ctcggggggc cggagagcgc gtgggcgagc ttgtcgtccc
    10501 agcgcgcgga gtcccagagc tcgaagtagt ggccctggcc gacgagcatg gcgtcgcgct
    10561 cgagccgggc gtgctggcgc agcgcgcccg agagcaggat gcggccggaa ccgtcgggct
    10621 gcacgtgctc ctcgaagccg agcagcagtc gcttccacca gctcgcctgc gggtgaaagc
    10681 tgggaaaaga ctgcgcctgc gtgcgcactg gctcccaggc ggcgggggga tacaggagca
    10741 cgcagccctc ggggtgcgcg gtgagcacga ggtggcgcgc gtcagcgagc agcgcggcgc
    10801 gatggcgcgt ggggatggcg agccggccct ttgcgtccag cgtgaccacc gttgcgccgt
    10861 ggaacgttcc acccatgccc cgttttctcc aaggcggacg gggcatgaac cacgcctccc
    10921 cgtgttccgc cacttttttc tacctttttc cactctatgc gacaaaaggg gcggcggtca
    10981 aggcaattct ttttgcctga caatgactta gctgcgtttc ctgggggcgt ttcttgaaat
    11041 aaaactacta tatatctcag tagcttataa aagctactga aagtgctgtc ttagaactac
    11101 ggccttagcc ggcggccaga cggtcgagct tcgcggcctc cacgcccagc ttggccccgg
    11161 ccg
//
LOCUS       DBNHKE010000229        35506 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_995792, whole genome shotgun sequence.
ACCESSION   DBNHKE010000229 DBNHKE010000000
VERSION     DBNHKE010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 35506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 35506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_995792"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="AC16O9_15615"
     CDS             complement(<1..866)
                     /locus_tag="AC16O9_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6518083.1"
                     /translation="MTRVAPLAPALAEAARATARVAAGRSLDEPTEQGEARSAVLDLC
                     YGTLRRYGRVHALVRALSRRGDAAPQVQALLWCALYALESGRYAEYTVVDQAVRACAL
                     LEHWPARGYVNGVLRSYLRDRDALQRRVLADDEARYQHPRWWIERLRRELPQHWQPVL
                     EAGNAHPPMCLRVNRRRADPAAYLAQLAAAGLPARSVGAYALLLDKPVPVARLPGFAE
                     GQVSVQDAGAQRCASLLDLAPGQRVLDACAAPGGKCAQMLEAAEVEMTALDADPARAA
                     RIAPGLARLGLSA"
     gene            complement(863..1786)
                     /gene="fmt"
                     /locus_tag="AC16O9_15620"
     CDS             complement(863..1786)
                     /gene="fmt"
                     /locus_tag="AC16O9_15620"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6518084.1"
                     /translation="MRLVFAGTPAFAERALAALLDAGHDLALVLTQPDRPAGRGLRPA
                     QSAVKQLALARGLDVLQPATLRDLDVQARVAAARPAFLVVAAYGLLLPQWALDVAPGG
                     ALNIHASLLPRWRGAAPIQRALLAGDAETGITIMRMDAGLDTGPMLAQERIAISAGDD
                     AQSLHDRLAALGARMIVAALAEAAAERVRAVPQSAQGVTYARKIEKRETEIDWRRPAS
                     EIERAVRALRPVPGAMARLRGETIKIWRAAVRGDAGDAGTVLAADAAGVLVACGEGAL
                     LATELQRAGGKRLAAAEFLRGCAIARGERLQ"
     gene            complement(1794..2297)
                     /gene="def"
                     /locus_tag="AC16O9_15625"
     CDS             complement(1794..2297)
                     /gene="def"
                     /locus_tag="AC16O9_15625"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6518085.1"
                     /translation="MALLNILRYPDARLHKTAAPVTVFDEALKKLVRDMAETMYAAPG
                     VGLAASQVDVHKQVIVLDVSERHDSLVVLVNPEIVEASGVSDIEEGCLSVPGVYDIVE
                     RAERVKVRAFDQNGNPFTLEAQGLLAVCIQHEMDHLKGKVFVEYLSQLKQQRVRAKLA
                     KQLRKSA"
     gene            2443..3630
                     /locus_tag="AC16O9_15630"
     CDS             2443..3630
                     /locus_tag="AC16O9_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6518086.1"
                     /translation="MHDQLRKSITALVFALSGLASSDALTQVPPIPQTPLAFKADAPD
                     RYVVVRGDTLWGISERFTDSPWRWPEIWNFNREQIRNPHWIYPGDVIVLDRVSGTLSI
                     AGADAGAPGTGAGAPVAPSDAGASAPAGRPDLGVGVGSVVGTVKLSPRVRAESTARDA
                     IPSIPPSAIEPFLSRPLVIEPDGLDNAPTIIATEDNRVIIEAGNQAYVRGMGDSKEEN
                     WFIYRRGKALVDPDTNVTLGYEAIYLGTARVTRAGDPATVRLTTVTQEVGRGDKLLPV
                     GVPEVPKYAPHAPAVFMQGRVIGIYGGLGKVGEAGPQQIITLNRGRADGVEVGHVFAL
                     YRPGPLIADASARTGGKPATFKLPDERYGLAFVFRIYDRVSYALVMRISKPVNPLDVV
                     QTP"
     gene            3685..4773
                     /gene="dprA"
                     /locus_tag="AC16O9_15635"
     CDS             3685..4773
                     /gene="dprA"
                     /locus_tag="AC16O9_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6518087.1"
                     /translation="MSKDPGLASWLQLTLTPGLGPAAIRGLLAQFGLPEKVLSAPRVQ
                     LERVGGPAAAALLQPDAVAAAVENALRWAEAPGHAVVTLADAAYPKLLLEIADPPPLL
                     YAVGRLELLARPALAIVGSRNATAQGMRNAEQFAAAFSAAGLTIVSGLALGIDAAAHA
                     GGLAAEGSTIAVLGTGVDVIYPRQNATLARRIAESGLLLSEFALGSAGAAHHFPRRNR
                     LISGLSQGCLVVEAAVASGSLITARAAAEQGREVFAIPGSIHSPLSKGCHALIKQGAK
                     LAESAEDVLAELTTFRRIATASTRAAAPQAADEEPLLAHMGFDPVDVDSICARSGLTV
                     ERVNADLLRLELGGRIAALPGGKYQRLN"
     gene            4797..5255
                     /locus_tag="AC16O9_15640"
     CDS             4797..5255
                     /locus_tag="AC16O9_15640"
                     /inference="COORDINATES: protein motif:HMM:NF016264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smg family protein"
                     /protein_id="HIR6518088.1"
                     /translation="MFDILVFVFENCRQADVSQAPERVARKLSAAGFDDTEISAALSW
                     LAGVVRAPQRSFAPLPESGTAFRAFAPKELAKLDAECRGFLLFLERSGILSPASREHV
                     IERALATAGDTLSLDQLKLIVVMVLWSQQAPASQLVAEELLSARGERLPN"
     gene            5354..7915
                     /locus_tag="AC16O9_15645"
     CDS             5354..7915
                     /locus_tag="AC16O9_15645"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIR6518089.1"
                     /translation="MTKKLIIAEKPSVAADIARALSGFTRKGDYFESNDYVLSSAVGH
                     LLELAVPEEFDVKRGKWSFAHLPLIPPRFELAPIERGAERLNLLLRLLKRKDVDGLIN
                     ACDAGREGELIFRYIVQHARIKKPIQRLWLQSMTPASIRDGFEGLRADKELLPLADAA
                     KSRSEADWLVGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLTILVEREEKIREFKPRD
                     YWEVHARFGARAGEYPGRWFDPEFKRDDDAERKAERLWQQRAAEAVVAACRGKPGSVT
                     EETKPTTQASPLLFDLTSLQREANGRFGFSARGTLALAQALYERHKVLTYPRTDSRAL
                     PEDYIATVKKTMDMLGGDREYAPFAKSVLKQGWVKPNRRIFDNSKISDHFAIIPTLQA
                     PKHLNDAERKLYDLVVRRFLAVFHPSAEFLQTTRITLVGEHRFRTEGKVLQNPGWLAV
                     YGRTAQDESANLPAVAAGEKVATLEVAAMPLQTKPPARFSEATLLSAMEGAGKLVEDD
                     ELRAAMEAKGLGTPATRAAIIEGLIREEYIHRVGRELVPTPKAFSLLFALKHFGVTEI
                     TSPELTGDWEFKLKQMERGKLKREEFMEHIEQVTRDLVERIRNGDIPDTAFATVSAPC
                     PKCGGTVQENYRKFQCQKCDFSIWKVTSGREWSLEEVAELITKRYVGPLTGFRSRMGK
                     PFSAALKLNDELRPEFDFGQGSAAGEDEPAPDFSAQDSLGACPKCGARVFEHGVAYLC
                     EKSVGNERNCDFRTGKIILQQPVEREQVHKLLATGRTDLLTRFISKKGRPFKAFLVKT
                     PGGKIGFEFQARASKSGAKPKPEAPLAEVKPMPKKKPAPKAPAKPTRRGSARRAR"
     gene            complement(7881..10301)
                     /gene="gyrB"
                     /locus_tag="AC16O9_15650"
     CDS             complement(7881..10301)
                     /gene="gyrB"
                     /locus_tag="AC16O9_15650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR6518090.1"
                     /translation="MGEPEQHRPEPGEYDSDSIKVLKGLEAVRKRPGMYIGDTSDGTG
                     LHHMVFEVVDNSVDEALAGFCDEIVVTIHTDNSISVLDNGRGIPTGIKEDDELRRSAA
                     EIVMTELHAGGKFDSNTYKVSGGLHGVGVSVVNALSSWLRLTVWRDGKVHQLEFADGV
                     PTAPLHVTGTTEKRGTEVHFLASRQIFVNVDYHYDILARRLRELSFLNNGVRIVLVDQ
                     RSGKEENFAFVGGVKGFVEYMNRAKSVLHPNVFSAQGEKDGISVDVAMQWNDSYQENV
                     ICFTNNIPQKDGGTHLTGLRAAMTRVLNKYIEESELAKKARVETTGDDMREGLACVLS
                     CKVPEPKFSSQTKEKLVSSEVRPAVEEIIAEKLRQYLDERPNDARIITSKIVDAARAR
                     EAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCELYLVEGDSAGGSAKQGRDRKF
                     QAILPLKGKILNVERARFDKLLSSAEIATLITALGCGIGKEEYNADKLRYHRIIIMTD
                     ADVDGSHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKSKLGKEERYLKDEHELNEFM
                     LRQALVDAALVPAANREPIRGEALAELAKSYLLAEAVIERVARWIDHDSLQALLRGAQ
                     VDLSRRDLAEASVESLAAAMPRNGVHVHADFDPKTERHQVRIERTRHGNMRVTAIDAE
                     FVQSGDYAQIRSTAELLRGLIGVGAYVQRGERKQPVKDFAEAMHWLLAEVQKSMVLQR
                     YKGLGEMNPTQLWETTMDPSTRRLLRVQIEDGIAADEIFTKLMGDEVEPRRAFIESNA
                     LGVRNLDV"
     gene            complement(10316..11422)
                     /gene="dnaN"
                     /locus_tag="AC16O9_15655"
     CDS             complement(10316..11422)
                     /gene="dnaN"
                     /locus_tag="AC16O9_15655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR6518091.1"
                     /translation="MTLVKAKRDELLAPLSAVSGIIERRHTLPILSNVLIDRADGVLS
                     FLATDIEIQITARSTLAAGGEARPVTVGARKFVDILRALPEGAEVTVQQQEKRLQLKA
                     GKSRFVLQTLPAEDFPRLARPSGEVARFALAQKDLRRQLALVQYAMAQQDIRYYLNGL
                     LLLIEDRQLRLVATDGHRLAYAALALPASLPRLEVIVPRKTVLELSKLLTDVEDEVKI
                     ELSATQAAFSFGSVELVSKLVDGKFPDYTRVIPTQHKNLVTLEREALRQALQRAAILS
                     NEKFRGVRWVLTDGSLKIVSSNAEQEEAQEELEVTYGGDALDIGFNVNYLLDVLNNVP
                     AAEVQCRFGDAASSALLQFPAEPEFKYVVMPMRI"
     gene            complement(11621..12952)
                     /gene="dnaA"
                     /locus_tag="AC16O9_15660"
     CDS             complement(11621..12952)
                     /gene="dnaA"
                     /locus_tag="AC16O9_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR6518092.1"
                     /translation="MQSLWDACLRRLEQELPAQQFNTWIRPLAADDAAAEPGTLVLSA
                     PNRFVLELVRERFAARIERLVTEASGREFGLRLVLARGGEAAAQREPAVRVNAVPPPA
                     LERARLNRLFTFDSFVTGKANQLARAAAIQVAENPGISYNPLFIYGGVGLGKTHLLQA
                     IGNHLAEQRPQARLRYTHAEQYVTDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQFFSN
                     KGRTQEEFFYAFNALVEAHKQIVISCDTYPKEIAGMEERLISRFGWGLTVAIEPPELE
                     MRVAIVLKKAEAEAAQISEEIAFFIAKHIRSNVRELEGALKKVLAFARFTGREITLEM
                     AKEALRDILNVASRQISVDGIQKTVAEYFKIKISDMHSKKRSRNVARPRQVAMSLAKD
                     LTPMSLPEIGEAFGNRDHTTVLHACRTISTLRKKDSVLNRDYLVLEQVLKG"
     gene            13162..13296
                     /gene="rpmH"
                     /locus_tag="AC16O9_15665"
     CDS             13162..13296
                     /gene="rpmH"
                     /locus_tag="AC16O9_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6518093.1"
                     /translation="MKRTYQPSKTRRARRHGFLVRSRTASGRSVLRARRARGRKRLSV
                     "
     gene            13310..13666
                     /gene="rnpA"
                     /locus_tag="AC16O9_15670"
     CDS             13310..13666
                     /gene="rnpA"
                     /locus_tag="AC16O9_15670"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6518094.1"
                     /translation="MPARGKPARYGFGAERRLARSAEFERLLSRGTRRSFSGYTFYLE
                     RRAEGPPRLGILVSRRHAAKATERNGIKRCIREAFRLEQARLGALDVLVRPPYGARAS
                     AAMLTRVRELFGRLEP"
     gene            13663..13887
                     /gene="yidD"
                     /locus_tag="AC16O9_15675"
     CDS             13663..13887
                     /gene="yidD"
                     /locus_tag="AC16O9_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6518095.1"
                     /translation="MTGLLRLALRGYQLLVSPLLGPSCRFHPSCSQYADEALARHGLL
                     RGGWLAGRRLCRCGPWHPGGYDPVPQRPRD"
     gene            13906..15570
                     /gene="yidC"
                     /locus_tag="AC16O9_15680"
     CDS             13906..15570
                     /gene="yidC"
                     /locus_tag="AC16O9_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR6518096.1"
                     /translation="MDTQRLILFFIFSFSLLLLWDAWQKEQQPKTAPAPAAQSAPGVP
                     TPAKPLAAPPPPSAAPALAPAAAAVPGAASAPEQSEIVQVSTDLLVADISTLGASLAR
                     LELRNHKDANDSTRNFVLLGAEHAYSAQSGLIGEGMPNHRSAWRALPGERTLGAGADK
                     LQVRFAADGPPGVAVEKVYTFRRNSYLVDVAFELKNAGGAAVAAYAYYQIVRDDKAPA
                     HESRMMYVYTGPVYYTLERAFQKVDFSDIEKGKAELPKEARDGWIGMMQHYFVSAWLP
                     PDKAAREFYARKLPDGLYAAGVILATPQAAPGDAASVSSQLFSGPQEQKRLKQAAPGM
                     DLVVDYGWLAIIAWPLFAVLEFFHRWSGNWGVAIILLTITIKLLFFPLSAASYKSMAK
                     MKLVTPRLTKIREMYGNDRAKMNQAMMELYKTEKINPLGGCFPILVQIPVFIALYWVL
                     LAAIELRHAPFIFWIKDLSAQDPYYVLPVLMAASMVLQTRMNPVPPDPVQAKVMQFMP
                     YVFSVFFFFFPAGLVLYWLVNNILSIAQQWQIQRMFDRDKTAHAKR"
     gene            15557..16888
                     /gene="mnmE"
                     /locus_tag="AC16O9_15685"
     CDS             15557..16888
                     /gene="mnmE"
                     /locus_tag="AC16O9_15685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR6518097.1"
                     /translation="MPSADPPGRAPDTIAAIATPAGRGAIGIVRVSGARAPEIARQVL
                     GRIPPPRIATLVWARDARGELLDEGLALYFPAPYSYTGEPGLEFHGHGGPTVLQLVLT
                     ACLDAGARLAEPGEFTRRAFLNGKLDLAQAESVADLIDAASREAARSALRSLQGEFSA
                     AVDALVAQLVELRALTEAMLDFPDEDVETLHRDDAAARLARARAALADVVEKSRQGSL
                     LRSGIHVVLAGRPNVGKSSLLNRLAGEERAIVTPIAGTTRDALRESIQIEGVPLVIID
                     TAGLRPSTDPVERLGIERTQRELERADIVIVIFEAGEMHAPLDAVPAGTALIEVYNKI
                     DLAPGFVPPAGALAVSAMTGAGMDALRRAILQAAGWSATGETVFLARERHLRALRRAV
                     AHLEAAAREPERWELFAEELRLAQVALAQITGEFTADDLLGEIFGRFCIGK"
     gene            17056..18894
                     /locus_tag="AC16O9_15690"
     CDS             17056..18894
                     /locus_tag="AC16O9_15690"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HIR6518098.1"
                     /translation="MSAPAPDPSDTSNAPAWIRHRGLRQWVTDIARLAKPDRIVWCDG
                     SQAEYDRLCAELVRAGTFIKLNEKLRPNSYLARSHPSDVARMEDRTFICSGTKEQAGP
                     TNNWVEPAEMRTTLKRLFDGCMRGRTMYVMPFSMGPIGSHIAQIGVEISDSAYVAVNM
                     RIMTRMGRAVADWLGEEGDYVPCVHSVGVPLAPGAKDVPWPSNEKEKWIVHFPETREI
                     WSYGSGYGGNALLGKKCLALRIASVMARDEDWLAEHMLVLGVESPAGEKSYVAAAFPS
                     ACGKTNFAMLIPPATYQGWRVTTIGEDIAWIKPGHDGRFYAINPEAGFFGVAPGTSQA
                     TNPNAMKTLAANVIFTNVALTPEGDVWWEDMTETPPARLIDWRGKEWTPGCGRVAAHA
                     NARFTVPAAQCPSMDPKWEDSGGVPIAAFMFGGRRSGTVPLVVEAPTWEDGVYMGATL
                     GSETTAAITGKVGVVRRDPMAMLAFCGYNIGDYFAHWLKMGKAAHHPPRIFRVNWFRK
                     DANGKFVWPGFGENMRVLEWIVKRCHGQAQGVDTALGIIPRHEDLNWRGLERFTREQY
                     EEIVRVDAAAWKKEVAAHDDLLGKLGAHLPQELALRRSRLHERLAA"
     gene            18875..20233
                     /locus_tag="AC16O9_15695"
     CDS             18875..20233
                     /locus_tag="AC16O9_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6518099.1"
                     /translation="MRGSPPEVTRVFHRNPRERYPVAVRGDGAYLIDRDGKRYLDASG
                     GAAVSCLGHSDAVVVRAIQEQLARLPFAHTSFFTNEPMEALADALVARAPESLQKVYF
                     VSGGSEAMEAALKLARQYFVEKGEPQRRHVIGRRQSYHGNTLGALAVGGNQWRRRQFE
                     PLLVDVTHVSPCYAYRGKQAGETDEAYGKRLAGELEGEIQRLGTQSVIAFVAETVVGA
                     TLGAVTPVPGYFKRVREVCDRYGVLLILDEVMCGMGRCGTLWAFEHEGVVPDLVAIAK
                     GLGAGYQPVGAVMVSGEIHDRIVAGSGFFQHGHTYLGHAAACAGALAVQRRLHEDGLL
                     ARVAPLGALLEQRLHAAFDAHAHVGEIRGRGLFWGLELVAEKSSKTPFEPGQRVHARV
                     KKAALDAGLMCYPMGGTLDGARGDHVLLAPPFIVEERQLDELVAKLGAAIDAAIRARV
                     AA"
     gene            20283..21245
                     /locus_tag="AC16O9_15700"
     CDS             20283..21245
                     /locus_tag="AC16O9_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6518100.1"
                     /translation="MRTLSALAASLIVAGAFATSAQAQQKYMTIGTGGVTGVYYPAGG
                     AVCRLVNKDRAKHGIRCSVESTGGSVFNINTIKAGELDMGVAQSDVQFNAAKGKQQFK
                     EPFGELRAVFSLHPEPVTVVVRKESNINSFADLKGKKFNVGNPGSGTRATLDELIGAM
                     GWNLSAFALASELKADEHGPALCDGKIDGFFYLVGHPSANIQDPTTVCGAKLVSVTGS
                     AVDKLVSANPYYAHATIPGGLYPGNPQDTKTYGVLATVVSSTRVPADTVYQVVKAVFD
                     NFDEFKKLHPAFQVLKPENMIKDGLTAPLHDGAVRYYKEKGWLK"
     gene            21321..23912
                     /locus_tag="AC16O9_15705"
     CDS             21321..23912
                     /locus_tag="AC16O9_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR6518101.1"
                     /translation="MAKTAPAPSSDLKQLVADSDTGGRAAGGIAGKLIFALALGWSLF
                     QLWYASPLPFTFGWGVLNDTEARSLHLGVGLLLAFLCYPTLRTPSQRNRIPWFDWIVG
                     LAAAVAGSYFLFFYAELATRPGQPNLQDIVVASAGIVLLLEATRRAVGLPMTILAVIF
                     LGYIMLGQYLPDVIAHKGASWERMLVHQWLTTEGVFGVALGVSVGYIFIFVLFGSLLD
                     KAGAGNYMMQVSFAMLGHLRGGPAKVAVVSSGLNGLISGSSVSNVVSGGIFTIPLMSK
                     AGYGGVKAGAIETASSVNGQIMPPVMGAAAFLMVEYVGIPYTDVIKHAFLPALISYIA
                     LFYIVHLEALKLGLEPTAAARARPLHQKLTAWGLGVTGTIVVCSVIYWVAEAAKNLFG
                     PVAAPWVLGLLLAALYVYTVWVAARCPDLPQDIDVDNPILPDTWPTVKAGLHFLIPIG
                     VLIWCLMIEELSPALSAFWATASLVALMATQRPLTALFRGKRQMAKAARIGAVEVVEG
                     LNDGARNMISIAIATATAGIIVGGITLTGLGFRMTDFVEVVSQGNVFAMLLFTAFVCL
                     VLGLGVPTTANYILVATLMAPVIVELGAQSDLVIPLIAVHLFVFYYGIMGDITPPVGL
                     ATFAAAAISGEDAIKTGVQGAVYALRTVVLPFVFIFNPMLLLIDVRSWWEVLLVAFAA
                     TVASCVFSAATLGWFQTRCRWWEIVLLLVGTFALFRPDYFMDKLYTPYTQVPAKEIFR
                     VAGELGEKERLVLVIKGTTIEGEDVRKTVAVQLAEPGDGRKRLAAAGLTIMALGEEVR
                     VAAVRFGSRARKSGFQQGFEVAAVKVPTDRPSEHWVYIPALALIGFVYFWQRRRGAAG
                     GRRRRPS"
     gene            complement(23909..24811)
                     /locus_tag="AC16O9_15710"
     CDS             complement(23909..24811)
                     /locus_tag="AC16O9_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR6518102.1"
                     /translation="MKARIATIQYMLRPIGDWREFERQVRFVLGAAGDYRPQFVLLPE
                     IFTTQLLSALPRQDVHAAIRALDQFTARYQDLMCEHAERGGYYLIGGSHPTLRDGKLY
                     NTSYLFSPRGEMHTQDKTHRTRWEREQWKTTPGDHLRLFETEFGDIAILVCYDVEFPE
                     LARMVCDAGADILFVPSSTDDRQGFWRVRYCCHARAIENQVYVAMASTVGSLPVEGLG
                     LHYGQASILTPSDYPFSRDGIAAEGVANTEQIVIADVDLELLFSNRVDGTTIPLYDKR
                     AELYASKVEIIRTVPAGKQKVGAI"
     gene            complement(24808..25467)
                     /locus_tag="AC16O9_15715"
     CDS             complement(24808..25467)
                     /locus_tag="AC16O9_15715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6518103.1"
                     /translation="MRPTPYVRHMQADDLARVKEIQDSVYPGIGGWRMEQLESQLRVF
                     PQGQLVACIDQRVVGAASSLIVKWDSYGTHHTYREVTAAGMFTTHDASARTLYGAEVF
                     ADPRSRRLGIGRALYRARRRLCRALNLRRIMSCGRMPNYHLVADRMSPEEYSARVIWG
                     DLEDPVLMFQMSEGFHWCGVVRDYLPSDKESCGHANIIVWLNDRYRADQPTAIPKGPI
                     L"
     gene            complement(25494..26672)
                     /locus_tag="AC16O9_15720"
     CDS             complement(25494..26672)
                     /locus_tag="AC16O9_15720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518104.1"
                     /translation="MISLARYASLLALPDVPQAFVSSFIGRIPIGIAGLAILLLVQSV
                     EGSYAQAGLIGATYVAGLAVAAPLLGRLIDRIGPRRVLAACALLYPAALLALIAALLG
                     RAPTAAGLLFAALAGASFPPVTACQRAWLRQKLGDDALLTAALSLDALMVELVFIAGP
                     LLVALLVAVFSPAAAVLAAALCGLLGSLQFMRTRALASWTRATHFSGAFLGPVAEPRF
                     ALLLVLVVCYASVFGLVEIGVAAYAAESGRPALAGVLLGVMSVGSAAGALAYGTRHWH
                     LALARQFSYSLAVMGLGIAPLALVSQTVPFALWCVIAGIAMTPTLIIQATLVAKSVKT
                     EHATEGFTWSATALLAGVGIGLAGGGLLLEQGSVSTLFLLATLVSVAAALLARASLTV
                     "
     gene            26830..28722
                     /gene="mnmG"
                     /locus_tag="AC16O9_15725"
     CDS             26830..28722
                     /gene="mnmG"
                     /locus_tag="AC16O9_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR6518105.1"
                     /translation="MQFPSEFDVIVVGGGHAGTEAALAAARMGCSTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMALATDESGIQFRTLNRSKGPAVRATRAQADRV
                     LYRRAIRTRLENQPNLTLFQQAVDDIVLEGDRACGVVTQLGIRFRGRAVVLTVGTFLS
                     GRVHVGLENYAAGRAGDPAAVTLAARLREMHLPVGRLKTGTPPRLDGRTIDFTAMEEQ
                     PGDTPEPVFSFLGDRSMHPRQLPCWVAHTNERTHELIRGALDRSPMYTGVIQGVGPRY
                     CPSIEDKIHRFADKASHQIFLEPEGLETHEIYPNGISTSLPFDVQLQLVRSIRGLEQA
                     HILRPGYAIEYDYFDPRNLKSSLETKTVAGLFFAGQINGTTGYEEAAAQGLLAGINAG
                     LRVRGGAPWCPRRDEAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLMLREDNADLRLTE
                     AGRRLGVIDDSRWDAFCRKRDAITAEQERLEKLWVGPRAITEAEATRVLGQPLEREYT
                     LASLLRRPNVSYRSLMTLANTGFSVESDQVAEQIDIQAKYQGYVERQKAEVERRAQHE
                     DHLLPHALDYASVRGLSKEVQQKLQDHRPETVGQAARISGVTPAAIALLLVHLKRVTT
                     LQKKSA"
     gene            28719..29345
                     /gene="rsmG"
                     /locus_tag="AC16O9_15730"
     CDS             28719..29345
                     /gene="rsmG"
                     /locus_tag="AC16O9_15730"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6518106.1"
                     /translation="MTPAAALHSGLAELGQLLDDTAQQKLIAFIELLAKWNKTYNLTA
                     IRDPLQMISHHLLDSLAVRPHLPAGALADVGSGGGLPGIPLAIAEPQRRVTLNDANHK
                     KAAFLQQAVIELKLANADVHTGRVQAWHPSSPFACVIARGYAELAQFIASCRHLVSTG
                     GALAAMKGAYPKEELSRVPQDVECREVWRLQVPSLDAERHLVLCRCAP"
     gene            29342..30136
                     /locus_tag="AC16O9_15735"
     CDS             29342..30136
                     /locus_tag="AC16O9_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6518107.1"
                     /translation="MSETRIVAVVNQKGGVGKTTTSVNLAAALARAGQRTLLVDLDPQ
                     GNATMGSGIDKRAASRTVYHVLLGLGEIAKIRLRSEQGGYDLLPANRELAGAEVELVE
                     LNNREGRLKAALASVRDEYDFVFIDCPPSLSLLTLNGLVAAQSVLIPMQCEYYALEGL
                     SDLVGTIKRVRANLNPQLEITGLVRTMYDARNTLAQQVSAQLETHFGDKVYRSLVPRN
                     VRLAEAPSYGIPAVVWDPESKGAQAYLALADEMLERERAGLPAAAA"
     gene            30133..30972
                     /locus_tag="AC16O9_15740"
     CDS             30133..30972
                     /locus_tag="AC16O9_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6518108.1"
                     /translation="MNQKRKGLGRGLDALLGGDEAPRDRLATLPVARIRPGRYQPRTR
                     MDQEALAELASSIQSQGLMQPVLVRPVDRERYELIAGERRWRAAQMAGLQEIPALVRE
                     VADESALAMSLIENIQRENLNPMEEAAGLQRLVDEFRMTHEQAADAVGRSRSATTNLL
                     RLLRLAKPVQGMVMEGVLEMGHARALLSLDGARQIEAANRIAARGLSVREAEALVNKM
                     QRGQTARRARKTDRDLARLEEEVSGRLGTTVEIRAGGKGRGKVIVHYSGLDHLDQLLK
                     KLR"
     gene            31058..31438
                     /locus_tag="AC16O9_15745"
     CDS             31058..31438
                     /locus_tag="AC16O9_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR6518109.1"
                     /translation="MNLSLGSLQRPIRTVLRWQLAATAALTLLAGVVAGAEGALSAAL
                     GGGVSVVAGGVSAWVAARGKARSAGEVLLGALLAEGVKIGLLVLLLWLVLATYKGVVA
                     PVFLGSFVATVLIFGMAFFVREND"
     gene            31443..32252
                     /gene="atpB"
                     /locus_tag="AC16O9_15750"
     CDS             31443..32252
                     /gene="atpB"
                     /locus_tag="AC16O9_15750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6518110.1"
                     /translation="MAAGKDFNASDYIQHHLTFLTKPVGDGGFWALNVDTLVTSVLIG
                     VLAMGAIWWVVRGATAGVPNRRQALVEMLIEFIDDQVKSIFHHGDRNTFVAPAALTVF
                     VWVLVMNAMDFLPIDIVVGPLEWMGLHAWRPVPTADINTTFALALSVWLLMIWFSIAS
                     KGLFGWIHELFCAPFGKNPLLWPANLLFNFIEYVSKPLSHSLRLFGNMYAGEIIFLLL
                     WLLGATGLFGTLIATVLGLGWAIFHILIVVLQAYIFMMLTVVYIAMAHEHH"
     gene            32294..32566
                     /gene="atpE"
                     /locus_tag="AC16O9_15755"
     CDS             32294..32566
                     /gene="atpE"
                     /locus_tag="AC16O9_15755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR6518111.1"
                     /translation="MEKLQMLAMIQAYTGIGIGLMIGVGAAGACIGVGIMCSRFLEAA
                     ARQPELTNSLQVKVFLLLGLIDASFIIGVGLAMMFAFANPLLAVIQ"
     gene            32592..33062
                     /locus_tag="AC16O9_15760"
     CDS             32592..33062
                     /locus_tag="AC16O9_15760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6518112.1"
                     /translation="MSFNLTLIAQALTFALFIWFTVKVVWPYMLRAIEARQKTIADGL
                     AAAEQGHKSLESSKRHAEDAISQARNRSSEILAQAEKRAVQMVEEAKNAARAESEREK
                     AAAKAEIEQEASRAREQLRDQVAALAVAGAEKILRREVDAKAHAELLESIKRQL"
     gene            33067..33603
                     /locus_tag="AC16O9_15765"
     CDS             33067..33603
                     /locus_tag="AC16O9_15765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR6518113.1"
                     /translation="MAEPSTIARPYAQAVFALADGAGKLAEWSAALANLAAVAEDGRV
                     REAVSDPNRSAAQAAGLLSSILAGKLSGDAANLVRVLAENGRLDVLQEIRTQYEALKN
                     EREGVIEAEVISAFEMNEAQVADLVARLENKTGRKVKMRVSVDQNLIGGVKVVLGDKV
                     IDGTARAQLGALEAALKA"
     gene            33613..35154
                     /gene="atpA"
                     /locus_tag="AC16O9_15770"
     CDS             33613..35154
                     /gene="atpA"
                     /locus_tag="AC16O9_15770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6518114.1"
                     /translation="MQLNPSEISELIKSRIQNLDASAQMRTQGTVVSVTDGICRVHGL
                     SDVMQGEMLEFPKSTFGLALNLERDSVGAVILGEYEHITEGDTVKCTGKILSVPVGPE
                     LLGRVVNSLGQPIDGKGPVKTRMTDVIEKVAPGVIARQSVSQPVQTGLKAIDAMVPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGKDLFCVYVAIGQKASTIANVVRKLEEHGAM
                     EYTIVVAASASESAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNPEYVEKFTKGEVKGKTGSLTALPVIETQA
                     GDVTAFVPTNVISITDGQIFLESDLFNAGIRPAINAGLSVSRVGGAAQTPIIKKLGGG
                     VRLALAQYRELAAFSQFASDLDEATRKQLERGQRVTELMKQRQYSPLSVAEMGVSLYA
                     ANEGFLDDVNVNKVVSFEDAMLAHIRSNNQDMLDKINESGDYNDEIAAEIRKAIEDFK
                     ASGVY"
     gene            35166..>35506
                     /locus_tag="AC16O9_15775"
     CDS             35166..>35506
                     /locus_tag="AC16O9_15775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR6518115.1"
                     /translation="MAGGKEIKTKIASVQNTQKITAAMEMVAASKMRKAQDQMFATRP
                     YAERIAEVVSHVARAHAEYKHPFLIDREIKRAGIIAISTDRGLCGGLNTNMFKKALNH
                     IGELDEQGIEI"
BASE COUNT         5954 a        11625 c        12046 g         5881 t
ORIGIN      
        1 gccgagaggc ctaggcgcgc cagtccaggc gctatgcgcg ccgcgcgcgc cgggtcggca
       61 tcgagcgccg tcatctccac ctctgcggcc tccagcattt gtgcgcactt gccgcccggc
      121 gcggcgcaag cgtcaagcac gcgctggccc ggcgcgagat cgaggagcga cgcgcagcgt
      181 tgcgccccgg cgtcctgcac cgaaacctgc ccttcggcga acccgggaag gcgggccacc
      241 ggcaccggct tgtccaggag cagcgcgtac gcgccgacgc tgcgcgccgg aaggccagcc
      301 gcggcgagct gggcgagata ggcggcggga tcggcgcgcc ggcggttcac ccgcaggcac
      361 atcggcggat gcgcgtttcc cgcctccaga accggctgcc agtgctgcgg cagctcgcgc
      421 cgcaggcgct cgatccacca gcgcgggtgc tggtagcgcg cctcatcgtc ggcgagcacg
      481 cgccgctgca gggcatcgcg atcgcgcagg tagctacgca gcacgccatt gacgtagccg
      541 cgcgcgggcc aatgttcgag cagcgcgcag gcgcgcaccg cctgatcgac gaccgtatat
      601 tcggcatagc gtccgctttc cagggcgtac agcgcgcacc agagcagggc ctgtacctgc
      661 ggcgcggcat cgcccctgcg cgacaaagcg cgcaccaggg catggacccg gccatagcgg
      721 cgcagggtgc cgtaacacag gtcgagcacc gcactgcgcg cttcgccctg ctcggtcggt
      781 tcatcaagac tgcgccccgc cgccacgcgc gccgtggcgc gcgccgcctc ggcgagcgcc
      841 ggtgccagtg gcgccacgcg cgtcattgca gccgctcgcc gcgagcgatc gcacagccgc
      901 gcaggaactc ggcagctgca agccgcttac ccccggcgcg ctgcagctcg gtggcgagca
      961 acgcgccctc gccgcaggcg acgagcaccc cggctgcgtc cgccgcgagc accgtcccgg
     1021 catcgccggc gtcgccgcgc acggcggcgc gccagatctt gatcgtctcg ccacgcagcc
     1081 tggccattgc gcccggcacg gggcgcaggg cgcgcacggc acgctcaatc tcggacgcag
     1141 gccggcgcca gtcgatctcg gtctcgcgtt tctcgatctt gcgcgcgtag gtgactccct
     1201 gcgcggactg aggtacggcg cgcactcgct ccgcggccgc ctctgcaagc gcagccacga
     1261 tcatgcgcgc gcccagggca gcgagtctgt cgtgcaacga ctgtgcatcg tccccggcgg
     1321 agatggcgat gcgttcctga gcgagcatgg gtccggtatc cagaccggca tccatgcgca
     1381 tgatggtaat tccggtttcc gcgtcgcccg caagcagcgc gcgctggatc ggcgctgcgc
     1441 cacgccagcg gggcagcagt gatgcgtgga tgttgagcgc gccgccgggc gcgacgtcta
     1501 gcgcccattg cggcagcagc aggccgtaag cggcgaccac caggaacgcc gggcgggctg
     1561 cagcgacacg cgcttgcaca tcgagatcac gcagcgtcgc aggctgcagt acatcgaggc
     1621 cgcgcgcgag cgcaagttgt ttgaccgcgc tttgcgccgg gcgcaggccg cgccccgcag
     1681 ggcggtcggg ttgcgtcagc accagcgcca ggtcgtggcc cgcatcgagc aacgcggcga
     1741 gcgcgcgctc tgcgaatgcc ggggtgcccg cgaagacgag gcgcaggaag gtgttaggcg
     1801 ctcttacgca gctgcttggc gagctttgcg cgcacgcgct gctgcttgag ctgcgatagg
     1861 tactccacga acaccttgcc cttcaggtga tccatctcgt gctgaatgca cacggcaaga
     1921 aggccctgag cctcgagcgt gaaggggttg ccgttctgat cgaaggcccg cactttgacg
     1981 cgctcggcgc gctcgacgat gtcgtacacg ccaggcaccg acaggcaacc ttcctcgatg
     2041 tcggagacgc ccgaggcttc aacgatctcg ggattgacca gcaccaccag cgaatcgtgg
     2101 cgctccgaca cgtcgagcac gatgacctgc ttgtgcacat ccacctgcga cgcggctagg
     2161 ccgacgccgg gcgcggcata catcgtctcg gccatgtctc ggaccagctt tttcagggcc
     2221 tcgtcgaaaa cggtgacagg agcggccgtc ttatgcagcc gggcgtcggg atagcgaagg
     2281 atgttcagca gagccatcgg aagggtaaat agcgttgcaa attgaataaa ttagatgcag
     2341 aatctaaggc accagataaa cagttcgagg tttgtgctac atttggatgg caaaggggca
     2401 tttcaagcca gcccgggaac aaggataagg ttcgggggca ccatgcacga ccaattgcgt
     2461 aagtctatca cagcgctcgt ttttgccctg agcggcctgg ccagcagcga cgcgctgacg
     2521 caggtcccgc cgatcccgca aacccccctg gcgttcaagg ccgacgcccc cgatcggtat
     2581 gtcgtggtgc gcggcgatac gctgtgggga atctcggagc gtttcacgga ttcgccgtgg
     2641 cgctggcccg aaatctggaa cttcaaccgc gagcagatcc gcaatccgca ctggatctac
     2701 ccaggggacg tcattgtcct tgatcgcgtg agcggcacgc tctcgatagc gggggcggac
     2761 gccggggcgc cggggacggg cgccggggcg ccggttgcgc ccagcgatgc cggtgccagc
     2821 gcgccggcgg gccggccaga cctcggcgtc ggcgtcggca gcgtagtagg cacggtcaag
     2881 ctctccccgc gggtgcgcgc cgagtccacg gcgcgcgacg cgatccccag catcccgcca
     2941 agcgccattg agccgttcct ttctcgaccc ctggtgatcg agcccgacgg cctcgacaat
     3001 gcgccgacca tcatcgccac tgaagacaac cgcgtcatca tcgaggccgg caaccaggcc
     3061 tacgtgcgcg gcatgggcga ctccaaggaa gagaactggt tcatctaccg ccgcggcaaa
     3121 gcgctggtcg accccgatac caacgtgacc ctcggctacg aagcgatcta ccttggcacg
     3181 gcgcgcgtca ctcgcgctgg agatccggcg acggtgcgcc tcaccacggt gacgcaggaa
     3241 gtgggcaggg gtgacaagtt gctgccggtg ggtgtgcctg aggtaccgaa gtacgcgccg
     3301 catgcccccg cggtcttcat gcagggacgg gtgatcggca tctatggcgg cctgggcaag
     3361 gtcggcgaag ccggaccgca gcagatcatc accttgaatc gcggacgcgc tgacggcgtc
     3421 gaggtcggcc atgtgttcgc gctgtaccgg ccgggaccgc tcattgccga tgcctcggcc
     3481 aggacagggg gcaaaccggc gacgttcaag ctgcccgacg agcgctatgg ccttgcgttc
     3541 gtgttccgca tctacgaccg cgtttcctac gcgctggtga tgcgcatctc caagccggtc
     3601 aatccgttgg atgtggtgca gacgccctga agcgcgctcc ggcgcggtgc gcctgcgccg
     3661 gcccgaagac accccgccgc cgggatgagc aaggacccgg gcctggcgag ctggctgcag
     3721 ctcaccctta cgccgggcct cggtcccgcc gccatccgcg gtctgcttgc ccagttcggc
     3781 ttacctgaga aggtactttc tgctccgcgc gtacagctcg aacgcgttgg cggtcctgcc
     3841 gccgctgcgc tgctgcagcc cgacgctgtc gctgccgccg tcgagaacgc gctgcgctgg
     3901 gccgaggcgc ccggtcatgc cgtcgttacg ctggcggacg ccgcctatcc caagctgctg
     3961 ctcgagatcg ccgatccgcc gccgctgctc tacgccgtcg gccgtctcga gttgctggcg
     4021 cgcccggcgc tcgccatcgt cggcagccgc aacgccactg cgcaaggcat gcgcaacgcc
     4081 gagcagtttg cggcggcatt cagcgccgcc ggactgacca tcgtgtcggg actggcgctg
     4141 ggcatcgacg ctgcggcgca cgccggcggg ctcgccgcgg agggctcgac catcgctgtg
     4201 ctcggcaccg gcgtcgacgt gatctacccg cgacagaatg cgacgctcgc caggcgcatc
     4261 gccgagtccg ggttgctgct ctcggagttc gcgctcggca gcgccggtgc cgcccaccat
     4321 ttcccgcgcc gcaaccgctt gatcagcggg ctgtcgcagg gttgcctggt ggtcgaggca
     4381 gccgtggcct ccggatcgct gatcacggcg cgcgcggccg ccgagcaggg ccgcgaggtg
     4441 ttcgccatcc ccggatccat ccactcgccg ctgtccaaag gctgccacgc gctcatcaag
     4501 cagggcgcca agctggccga atcggccgag gatgtactcg ccgagctgac cacgttccgg
     4561 cgcatcgcca ccgcgagcac tcgcgccgcg gcaccgcaag ccgcagacga ggaacccctg
     4621 ctcgcgcaca tgggcttcga cccggtggac gtggattcga tatgcgcgcg ctcgggactt
     4681 acggtagaaa gggtaaacgc ggacttgctg cgcctggagc tgggcgggcg cattgccgcg
     4741 ttgccgggcg gcaaatacca gcggcttaac tgagggtcaa ccaggagaat aacaacatgt
     4801 tcgacatcct cgtcttcgta ttcgagaact gccggcaagc cgacgtgtcg caagcgcccg
     4861 agcgcgtggc gcgcaagctc tcggccgcgg ggttcgacga cacggagatc agcgccgcgc
     4921 tctcgtggct cgcgggcgtg gtgcgcgcgc cgcagcgctc gttcgcgccg ctgcccgaaa
     4981 gcggcacggc gtttcgcgcg ttcgcgccga aggagctcgc caagctcgac gccgaatgcc
     5041 gcggcttcct gctgttcctc gagcgctccg gcatcctctc gccggcatcg cgcgagcacg
     5101 tcatcgagcg tgccctcgcg accgcgggcg acacgctgtc gctggatcag ctcaagctga
     5161 tcgtggtcat ggtgctgtgg agccagcagg cgcctgcgag ccagctggta gccgaagagc
     5221 tgctatcggc gcgcggcgag cgcctgccga actgacgcgc attcgcgcgc gcgggcgcgc
     5281 gcgcgcgaat aaatcgacgg gcggttgaaa gggcgcgagc tttgcgggta tcctctgcgg
     5341 cctttcgcgc cgcatgacca agaaactcat catcgccgag aaaccctccg tcgccgcgga
     5401 catcgcgcgc gcgctcagcg gctttacgcg caagggcgac tatttcgaga gcaacgatta
     5461 cgtcctttcc tcggccgttg gtcacttgct cgagctggcg gtgcccgagg agtttgacgt
     5521 caagcgtggc aagtggtcgt ttgcccacct gcccctgatc ccgccgcgct tcgagctcgc
     5581 gcctatcgag cgcggcgctg agcggctcaa cctgctgttg cgcctgctca agcgcaagga
     5641 cgtcgacggc ctgatcaatg cctgcgacgc gggccgcgaa ggcgagctca tattccgcta
     5701 catcgtgcag cacgcgcgga tcaagaagcc gatccagcgg ctgtggctgc agtcgatgac
     5761 gcctgcatcg attcgcgacg gcttcgaggg tttgcgcgct gacaaggagt tgctgccgct
     5821 cgccgacgcc gcgaagagcc ggtcggaggc cgactggctg gtcggcatca acggcacgcg
     5881 cgccatgacc gcgttcaatt ccaaggaggg cggcttttac ctgacgaccg tcggccgggt
     5941 gcaaacgccg acgctcacga ttctcgtgga gcgcgaggag aagatccgcg aattcaagcc
     6001 gcgcgactac tgggaagtgc acgcgcgctt cggcgccagg gcaggcgagt atccggggcg
     6061 ctggttcgat ccggagttca agagggacga cgatgccgag cggaaggccg agcggctgtg
     6121 gcagcaacgc gcggccgagg ccgtcgttgc cgcctgccga ggcaagccgg gcagtgtcac
     6181 cgaagaaacc aagccgacga cgcaggcctc acctctgctg ttcgatctga ccagcctgca
     6241 gcgagaggcc aacggccgct tcggcttttc tgcgcgcggg acgctggcgc tggcgcaggc
     6301 gctctacgag cgccacaagg tcctgaccta tccgcgcacc gactcgcgcg ccctgcccga
     6361 ggactacatc gccacggtga aaaagaccat ggacatgctg ggcggcgacc gcgagtacgc
     6421 cccgttcgcc aagtccgtgc tgaagcaggg ctgggtcaag cccaaccggc gcatcttcga
     6481 caacagcaag atctcggacc acttcgccat catcccgaca ctgcaggcgc cgaagcacct
     6541 gaacgacgcc gagcgcaagc tctacgacct cgtggtgcgg cgcttcctcg ccgtgttcca
     6601 cccgtccgca gaattcttgc agaccacccg catcaccttg gtcggggagc atcggttccg
     6661 taccgaaggc aaggttctgc agaacccggg ctggctcgcc gtctacgggc gcacggcgca
     6721 ggacgaaagc gcgaacctgc ccgcggtggc cgcgggcgag aaggtcgcga cgctggaggt
     6781 cgccgccatg ccgctgcaga ccaagccgcc ggcgcgcttc agcgaggcga cgctgctctc
     6841 cgccatggag ggcgcgggca agctggtgga ggacgacgaa ctgcgcgcgg ccatggaagc
     6901 caagggactc ggcacgccgg cgacgcgcgc ggcgatcatc gaagggctga tccgcgagga
     6961 gtacatccat cgcgtcgggc gcgagctggt gcccacgccg aaggcgttct cgctgctctt
     7021 cgcgctgaag catttcggcg tcaccgagat cacctcgccc gagctcaccg gcgattggga
     7081 gttcaagctc aagcagatgg agcgcggcaa gctgaagcgc gaggagttca tggagcacat
     7141 cgagcaggtg acgcgcgacc tggtcgagcg catccgcaac ggcgacatcc cggacaccgc
     7201 cttcgccacg gtgagcgcgc catgcccgaa atgcggcggc accgtgcagg agaactaccg
     7261 caagttccag tgccagaaat gcgacttctc catctggaag gtcacctcgg ggcgcgagtg
     7321 gtcgctggag gaggtggccg agctgatcac caagcgctac gtcggaccgc tgaccgggtt
     7381 ccgcagccgc atgggcaagc ccttcagcgc cgcgctgaag ctgaacgacg agctgcgccc
     7441 cgagttcgat tttggccagg gcagcgccgc cggcgaagac gagccagcgc ccgacttttc
     7501 ggcgcaggac agcctcggcg cctgccccaa gtgcggcgcg cgcgtgttcg agcacggcgt
     7561 cgcgtacctt tgcgagaaat cggtgggcaa cgagcgcaac tgcgatttcc gcaccggcaa
     7621 gatcatcctg cagcagccgg tcgagcgcga gcaggtgcac aagctcctcg ccaccggccg
     7681 caccgacctg ctgacgcgct tcatttccaa gaagggccgc ccctttaagg cgttcttggt
     7741 gaagacgccg ggtggcaaga tcggtttcga gttccaggcg cgagcgtcca agtccggcgc
     7801 aaagccgaag cccgaggcac ccctggcgga ggtgaagccg atgccgaaga agaaaccagc
     7861 gcccaaggcg ccggccaagc ctacacgtcg aggttccgca cgccgagcgc gttagactcg
     7921 ataaacgcgc ggcgcggctc gacttcgtcg cccatcagct tggtgaagat ctcgtccgcg
     7981 gcaatgccgt cctcgatctg caccctgagc agccgccgcg tcgacgggtc catggtggtt
     8041 tcccatagct gcgtggggtt catctcgccc agccccttgt agcgctgcag caccatcgat
     8101 ttctgcactt cggcgagcag ccagtgcatc gcctcggcga agtccttcac cggctgcttt
     8161 cgctctcccc gctgcacgta cgccccgaca ccgatcagcc cgcgcagcag ctccgcggtc
     8221 gaccggatct gcgcatagtc cccggattgc acgaactccg cgtcgatcgc cgtgacgcgc
     8281 atgttgccgt gccgcgtgcg ctcgatgcgc acctgatgcc gctcggtctt tggatcgaaa
     8341 tcggcgtgca catgcacgcc attgcgcggc atggccgcgg ccagcgactc gaccgaggcc
     8401 tcggctaggt cgcggcgcga caggtcgacc tgcgcaccgc gcagaagcgc ctgcagcgag
     8461 tcgtggtcga tccagcgcgc cacgcgctcg atcaccgcct cggcgagcag gtaggacttg
     8521 gccagctcag cgagcgcctc gccacggatc ggctctcggt tcgccgccgg caccagcgcc
     8581 gcgtccacca gcgcctggcg cagcatgaac tcgttcagct cgtgctcgtc cttcaggtag
     8641 cgctcctcct tgccgagctt ggacttgtag agcggcggct gcgcgatata gatgtggccg
     8701 cgctccacca gctcgggcat ctggcggtag aagaaggtga gcagcagcgt gcggatgtgc
     8761 gagccgtcga cgtccgcgtc ggtcatgatg atgatgcggt ggtagcgtag cttgtcggcg
     8821 ttgtactcct ccttgccgat gccgcagcct agcgcggtga tcagcgtggc gatctcggcg
     8881 gatgacagca gcttgtcgaa ccgcgcgcgc tcgacgttga ggatcttgcc cttcagcggc
     8941 aggatcgcct ggaacttgcg gtcgcgcccc tgcttggccg agccgcccgc cgagtcgccc
     9001 tccacgaggt agagctcgca cagcgcgggg tccttttcct ggcagtcggc gagcttgcca
     9061 ggcagtccca tgccgtccag cacgcccttg cgccgtgtca tctcgcgcgc cttgcgcgcc
     9121 gcttcccggg cgcgcgcggc atcaacgatc ttcgaggtga tgatgcgcgc gtcgttcggc
     9181 cgctcgtcca ggtactggcg gagcttctcg gcgatgatct cctcgaccgc cggacgcacc
     9241 tccgaggaca ccagcttctc cttggtctgc gaggagaact tgggctccgg caccttgcac
     9301 gagagcacgc acgccaagcc ctcgcgcatg tcgtcgccgg tggtctccac ccgagccttc
     9361 ttcgccagct cgctttcctc gatgtacttg ttgagcacgc gcgtcatcgc cgcgcgcaag
     9421 ccggtgaggt gcgtgccgcc gtccttctgc gggatgttgt tcgtaaagca gatgacgttt
     9481 tcttggtacg agtcgttcca ctgcatggcg acgtcgacgc tgatgccgtc cttttcgccc
     9541 tgcgcgctga aaacattggg gtgcagcacc gacttcgcgc ggttcatgta ctcgacgaaa
     9601 cccttcacgc cgccgacgaa ggcgaagttc tcctccttgc cggagcgctg gtcgacgagc
     9661 acgatgcgca cgccgttgtt caggaacgaa agctcgcgca ggcgccgcgc aagaatgtcg
     9721 tagtgatagt cgacgttgac gaagatctga cgcgaggcga ggaaatgcac ctcggtcccg
     9781 cgtttttcgg tagtgccggt gacatgcaga ggcgcggtcg gcacgccgtc cgcaaactcg
     9841 agctggtgta ccttgccatc gcgccagacg gtgagccgca gccagctgga gagcgcattg
     9901 acgaccgaca cgcccacgcc gtgcaggccg cccgacacct tgtaggtgtt ggaatcgaac
     9961 ttgccgcccg cgtgcagctc ggtcatcacg atttcggcgg ccgagcgccg cagctcgtcg
    10021 tcttccttga tgccggtcgg gatgccgcgg ccgttgtcca gcaccgagat cgagttgtcg
    10081 gtgtggatcg tcaccacgat ctcgtcgcag aacccggcga gcgcctcgtc caccgagttg
    10141 tccaccacct cgaacaccat gtggtgaagt ccggtgccat cggacgtgtc gccgatgtac
    10201 atgccagggc gcttgcgcac cgcctccagc cccttcagga ccttgatgct gtccgagtcg
    10261 tactcgccag gctcgggtcg atgctgttcg ggttcgccca cggtgctccc ttttctcaga
    10321 tgcgcatcgg catgacgacg tacttgaact cgggctccgc cggaaactgc agcagcgcgc
    10381 tggaggccgc gtcgccgaag cggcattgca cttcggcggc cggcacgttg ttcagaacat
    10441 cgagcagata gttgacgttg aagccgatat cgagcgcatc gccgccgtag gtgacttcga
    10501 gctcctcctg cgcctcttcc tgctcggcgt tggacgagac gatcttcagg ctgccgtcgg
    10561 tcagcaccca gcgcacgccg cggaacttct cgttcgacag gatcgcggcg cgctgcagcg
    10621 cctggcgcaa cgcttcgcgc tcgagggtca ccaagttctt gtgctgcgtc ggaatgacgc
    10681 gcgtgtagtc gggaaacttg ccgtcgacaa gcttggacac cagctccacg ctgccgaaac
    10741 tgaatgcagc ctgcgtcgca gacagttcga tcttcacctc gtcttcaacg tcggtgagga
    10801 gtttcgaaag ctcgagcacc gtctttcgcg gcacgatcac ttccagacgc ggcaggctcg
    10861 ccggcagtgc cagcgcggcg tacgcgaggc ggtggccgtc ggtcgccacc aggcgcagct
    10921 gccggtcctc gatcagcagc agcaggccgt tcaggtagta gcggatatcc tgctgcgcca
    10981 tggcgtactg caccagcgcg agctgccggc gcaggtcttt ctgcgccagt gcaaagcgcg
    11041 ctacctcacc gctggggcgg gccagacgcg gaaagtcctc cgccgggagg gtctgcaata
    11101 cgaatcggct cttgccggcc ttcagctgca ggcgcttctc ctgctgttgc acggttacct
    11161 cggccccctc ggggagcgca cgcaggatat cgacaaactt ccgcgcgccg acggttaccg
    11221 gccgcgcctc gccgcccgcg gccagggtcg agcgtgccgt gatctggatc tcgatgtcgg
    11281 tggcgagaaa cgacagcacg ccgtcggcgc gatcgatgag gacgttcgac aggattggca
    11341 gggtgtggcg acgctcgatg atgccgctca ccgcggaaag cggtgcgagc aactcgtcgc
    11401 gcttcgcttt gaccaaggtc attatttatc cttagtaata gttaatagag caggccgagt
    11461 tgtgccggga gcccgaaaaa gcctgcgcgc gcatccagtt gcgatccacg gcggcgcggg
    11521 acaagctgtg ggcggcatgt gcgcgactgt gcatagaaaa gcgctgcacg cgctgcacgc
    11581 gacttacgca cattcggcgc aacggaaatc cacaggctat tcatcccttc agcacctgct
    11641 ctaggaccag gtaatcgcgg ttcaagaccg agtctttctt gcgcagtgtg gaaatggtgc
    11701 ggcaggcgtg cagcaccgtc gtgtggtcgc ggttgccgaa cgcctcgccg atctcgggca
    11761 ggctcatcgg cgtcaggtcc ttggcgagcg acatcgccac ttgccgcggg cgcgctacgt
    11821 tgcggctacg cttcttggag tgcatgtccg agatcttgat cttgaagtac tcggcgaccg
    11881 tcttctggat gccgtccacg ctgatctggc gactggcgac gttcaggatg tcgcgcagcg
    11941 cctccttggc catctccaga gtgatctccc ggcccgtaaa gcgcgcaaat gcgagcacct
    12001 tcttcagcgc gccctcgagc tcacgcacgt tggagcggat gtgcttggcg atgaagaacg
    12061 cgatctcctc tgagatctgc gcggcctccg cttccgcctt cttcagcaca atagcgacgc
    12121 gcatctccag ctcgggtggc tcgatcgcca cggttagacc ccagccgaag cgcgagatca
    12181 ggcgctcttc catgccggcg atctccttcg ggtaggtgtc gcaggagatc acgatttgct
    12241 tgtgggcttc gaccagcgcg ttgaacgcgt agaagaactc ctcctgggta cggcccttgt
    12301 tcgagaagaa ctggatgtcg tcgatcagca ggaggtccag cgagtggtag taacgcttga
    12361 actcatcgaa cgatttctgc tggtaggcgc gcaccacgtc ggtgacgtac tgctcggcgt
    12421 gcgtatagcg cagccgcgcc tgcggccgct gctcggcgag gtggttgccg atcgcctgca
    12481 acaggtgcgt cttgcccagg ccgacgccac cgtagatgaa cagcgggttg taggagatgc
    12541 ccgggttctc ggcgacttgg atcgccgcgg cgcgcgccag ctgattcgcc ttgcccgtga
    12601 cgaagctgtc gaacgtgaac aggcgattca ggcgcgcccg ctcgagcgcg ggcggcggca
    12661 cggcgttcac gcgaaccgcc ggctcgcgtt gcgccgcggc ctcgccgccg cgcgccagca
    12721 ccagccgcaa tccgaactcg cgcccgctcg cctcggtgac caggcgctcg atgcgtgcgg
    12781 caaagcgctc gcgcaccagc tcgagcacga agcggttcgg ggccgatagc accagcgtac
    12841 ccggctctgc cgccgcgtcg tccgcggcca gggggcgaat ccaggtgttg aattgttgcg
    12901 cgggaagctc ctgctccaga cggcgcaggc aggcgtccca aaggctttgc atgttttctc
    12961 ggcggtctcg cgcagcgcgt tgttcggggg cttcaccggg gccgcgcttg gcgcgccggc
    13021 gaggataaat tttacccctt ccgcgccatg ttatccacag gattgacgcg ctcttggcgc
    13081 atggctcggt tcagcgcaag tatttgacaa tgctgcgcag cgcaggctct aataccgccc
    13141 tttttcgcag cgagcatgcc gatgaagcgc acttatcagc cgtccaagac tcgccgcgcg
    13201 cggcgccacg gatttcttgt gcgcagccgt accgcttcgg gccgctccgt gctgcgcgca
    13261 cgacgcgctc gcggccgcaa gcgcctctcg gtctaggccg cagaggagcg tgcccgctcg
    13321 cggcaagcct gcgcgctacg gcttcggcgc agagcgcagg ctggcgcgca gcgcggagtt
    13381 cgaacggctg ctgagccggg gtacgcggcg gagtttttca ggctatacct tttacttgga
    13441 gcgccgcgcg gaaggaccgc cgcggcttgg gatactcgtg tcgcgcagac acgcggcgaa
    13501 ggctacggag cgcaatggca tcaagcggtg catacgtgag gcattccggc tcgagcaggc
    13561 gcgccttggc gcgctcgacg tactggtgcg cccgccttac ggcgcgcgcg cctcggcggc
    13621 catgctaacg cgcgtgcgcg agctgttcgg caggcttgag ccatgaccgg cttgttgcgc
    13681 ttggcgctgc gcggttatca actcttggtc agccccctgc tcgggccttc ctgccgtttt
    13741 caccccagtt gctcgcagta cgccgacgaa gcgctggcgc gacatgggct tttgcgcggg
    13801 ggctggcttg ccggccgccg gctgtgccgc tgcgggccct ggcacccggg cggctacgat
    13861 ccggtgccgc agcggccccg ggactaaggc ggggtgcggg gcgcgatgga cacccaaaga
    13921 ctgatcctgt tcttcatctt cagcttctcg ctgctgctgc tgtgggacgc atggcagaag
    13981 gagcagcagc cgaagaccgc gccggccccg gcggcgcaat cggcgcccgg cgtgccgacg
    14041 ccggccaagc cgctcgccgc gcccccgccg ccgagcgcgg ctccggcact cgcgcccgcg
    14101 gcggcggccg tgccgggtgc ggcgtccgcg cccgagcaat ccgagatcgt gcaggtcagc
    14161 accgacctgc tggttgcgga tatcagcacg ctgggcgcct cgctcgcgcg actcgaattg
    14221 cgcaaccaca aggatgccaa cgattcgacg cgaaacttcg tgctgctcgg ggccgagcac
    14281 gcctatagcg cgcagagcgg gctgatcggc gagggaatgc ccaaccatcg ctcagcctgg
    14341 cgcgcacttc ccggtgagcg cacgctgggc gccggcgccg acaagctgca ggtgagattt
    14401 gccgccgacg gaccgcccgg cgtcgcggtg gagaaggtct acacgttccg ccgcaacagc
    14461 tatctcgtcg acgtcgcttt cgagctcaag aacgcgggcg gcgcggctgt cgccgcttac
    14521 gcgtactacc agattgtgcg cgacgacaag gcgcccgcgc acgagagccg catgatgtat
    14581 gtctataccg gtccggtgta ctacacgctg gagcgcgcgt tccagaaggt cgatttttcc
    14641 gacatcgaga agggcaaggc ggagttgccg aaggaagcgc gcgatggctg gatcggcatg
    14701 atgcagcact atttcgtgtc ggcttggctg ccgcccgaca aggccgcgcg cgagttctac
    14761 gcgcgcaagc tcccggacgg cctgtacgcc gccggcgtca tcctcgccac gccgcaggcc
    14821 gcgcccggag acgcggccag tgtctcgtca cagctgttct cggggcccca ggagcagaag
    14881 cgcctgaagc aggccgcgcc gggaatggat ttggtggtcg actacggctg gctcgcgatc
    14941 atcgcctggc ctctgttcgc ggtgctcgaa ttcttccacc gctggtcggg caactggggc
    15001 gtggccatta tcctgctgac gatcacgatc aagctgctct tcttcccctt gtcggcggcg
    15061 agctacaagt cgatggcgaa gatgaagctg gtgacaccgc gcctgaccaa gatccgcgaa
    15121 atgtacggca atgatcgcgc gaagatgaac caggcgatga tggagctgta caagaccgag
    15181 aagatcaacc cgctgggcgg ctgcttcccg atcctggtgc agatcccggt gttcatcgct
    15241 ttgtactggg tgctgctcgc ggcgatcgag ctgcgccacg cgccgttcat cttctggatc
    15301 aaggacctct cggcgcagga tccctattac gttctgccgg tgttgatggc agcctcgatg
    15361 gtgctgcaga cgcgcatgaa cccggtgccg ccggatccgg tgcaggcgaa agtcatgcag
    15421 ttcatgcctt atgtcttcag cgtgttcttc ttcttcttcc ccgccggcct ggtgctgtac
    15481 tggctggtca acaacatcct ttccatcgcc cagcaatggc agatccagcg catgttcgat
    15541 cgcgacaaga ctgcccatgc caagcgctga cccacccgga cgagcgcccg ataccatcgc
    15601 cgcgatcgcc acgcccgccg ggcgcggcgc tatcggcatc gtgcgcgtat cgggggcgcg
    15661 cgcgcctgaa atagcgcgtc aagttctcgg acgcataccg ccgccccgca tcgcgactct
    15721 ggtatgggcg cgcgatgcgc gcggcgagct cctcgacgag ggacttgcgc tctactttcc
    15781 cgccccgtat tcgtatactg gggagcccgg gctggaattc cacggtcacg gcgggccgac
    15841 ggtgctgcaa ttggtgctca cggcctgcct cgacgccggc gcgcggctcg ccgagcctgg
    15901 agaattcact cgccgcgcgt tcctgaacgg caagctcgat ctggcgcaag cggagtccgt
    15961 cgccgacctg atcgatgccg caagccgcga agcggcgcgc tcggcgctgc gctcgctaca
    16021 aggggaattt tccgcggccg tggacgcact ggtcgcgcag ctcgtcgagc tgcgcgcgct
    16081 gaccgaggcg atgctggatt ttccggacga ggacgtggag accctgcatc gcgacgatgc
    16141 ggccgcccgc ctggcgcgcg cgcgcgcggc gctcgcagat gtcgtcgaaa agagccggca
    16201 gggcagtctc ctgcgcagcg gcatccacgt cgtgcttgcc ggccggccaa atgtcggcaa
    16261 gtcaagcctg ctcaaccgcc ttgccggcga ggagcgcgcc atcgtcacgc cgatcgccgg
    16321 cacgacgcgc gatgcgctgc gcgaatctat ccagatcgag ggcgtgccgc tcgtcatcat
    16381 cgatacggcg gggctgcggc cctcgaccga ccccgtcgag cgcttgggca tcgagcgcac
    16441 gcaacgcgag ctcgagcgcg ccgacatcgt catcgtgatc ttcgaagccg gcgaaatgca
    16501 tgcgccactc gacgcagtgc cggcaggcac ggcgctgatc gaggtctaca acaagatcga
    16561 tcttgcgcct ggattcgtgc cgcctgccgg cgcgctggcc gtgtcggcga tgaccggggc
    16621 gggcatggac gcgctcaggc gcgccatcct gcaggccgcc ggctggagcg cgaccggcga
    16681 gaccgtgttc ctggcgcgcg agcggcatct gcgcgcgctg cggcgcgccg tggcacatct
    16741 cgaggccgcg gcgcgcgagc cggagcggtg ggagctgttc gcggaagaac tgcggctcgc
    16801 gcaggtcgcg ctggcgcaga tcaccggcga gttcaccgca gacgatctgc tcggcgagat
    16861 cttcggccgc ttctgcatcg gcaagtaagc gcgggccgcg cggggcgcca gtaagccatt
    16921 gttgctgcga gagtttatgt acgtatctcg gcttggcgac gcgcgaaggg cgagggcccg
    16981 ggttacaatc acgaaaaaca tcgagcaccg gctcagcgta cagctgggcc gtttgacttt
    17041 ctggagtcag cacgcatgag cgcgcctgca cccgatccta gcgacacatc gaacgccccg
    17101 gcgtggatac gtcaccgcgg cctgcggcag tgggtcaccg acatcgcgcg gcttgccaag
    17161 ccggatcgca ttgtgtggtg cgacggctcg caggccgaat atgaccgcct gtgcgccgag
    17221 ctggtgcgcg ccggaacgtt catcaagctg aacgaaaagc tgcgccccaa cagctacctc
    17281 gcgcgctcgc acccgagtga cgtggcgcgc atggaagacc gcaccttcat ctgcagcgga
    17341 acgaaggagc aggccgggcc gaccaacaat tgggtcgagc cggccgagat gcgaacgacg
    17401 ctcaagcgcc tgttcgacgg ctgcatgcgc ggccgcacca tgtacgtgat gccgttctcg
    17461 atgggtccga tcggctcgca catcgcgcag atcggcgtcg agatctccga ctcggcctac
    17521 gtcgccgtga acatgcgcat catgacgcgc atgggccgcg cggtcgcaga ctggctcggc
    17581 gaggagggcg actacgtgcc ctgcgtgcat tcggtaggcg tgccgctcgc cccgggcgcc
    17641 aaggacgtgc cctggccgag caatgaaaag gagaagtgga tcgtgcactt cccggagacg
    17701 cgcgagatct ggtcttacgg ctccggctat ggcggcaacg cgcttcttgg caagaaatgc
    17761 ctcgcgttgc gtatcgcctc ggtgatggca cgcgacgaag actggctcgc agagcacatg
    17821 ctggtcctcg gcgtggaatc gccggcgggc gagaagtcct acgttgcggc agcgttcccc
    17881 agcgcttgcg gcaagaccaa ctttgccatg ctgatcccgc ctgcgaccta ccagggctgg
    17941 cgcgtcacta cgatcggcga ggatatcgcc tggatcaagc ccgggcacga cgggcgcttc
    18001 tacgccatca acccagaagc cggctttttc ggcgttgcgc ccggtacttc gcaggcgacc
    18061 aatccgaacg ccatgaagac gctggccgct aacgtcattt tcaccaacgt tgccctcacg
    18121 cccgagggcg acgtgtggtg ggaggacatg accgagacgc cgcccgctcg cctgatcgac
    18181 tggcgcggca aggagtggac gccgggctgc ggtcgcgttg cggcccacgc caacgcgcgc
    18241 ttcaccgtgc cggcggcgca atgcccctcg atggatccga aatgggagga ctcgggcggc
    18301 gtgccgatcg cagcgttcat gttcggaggc cggcgctcgg gcaccgtacc gctggtggtc
    18361 gaggcgccga cctgggaaga cggggtgtat atgggcgcga cgctcggctc cgagaccacg
    18421 gccgccatta ccggcaaggt gggcgtggtg cggcgcgatc cgatggcgat gctcgccttc
    18481 tgcggctaca acatcggcga ttacttcgca cattggctga agatgggcaa agcagcgcat
    18541 cacccgccgc gcatcttccg cgtcaactgg ttccgcaagg acgcgaacgg caagttcgtc
    18601 tggccgggct tcggcgagaa catgcgcgtg ctggagtgga tcgtgaagcg ctgccatggc
    18661 caggcacagg gcgtcgacac ggcccttggc atcattccgc ggcacgagga cctcaattgg
    18721 cgcgggctcg agcgctttac gcgcgagcag tacgaggaga tcgtgcgcgt ggacgccgcc
    18781 gcgtggaaga aggaggtcgc ggcgcacgat gacttgctcg gcaagctcgg cgcgcacctg
    18841 ccgcaggagt tggccctgcg ccgctcacgc ctgcatgaga ggctcgccgc ctgaagtgac
    18901 ccgcgtcttc caccgcaatc cgcgcgagcg ctacccggtc gcggtgcgcg gtgacggcgc
    18961 gtacctgatc gatcgcgacg gcaagcgcta cctcgacgca tccggcggcg cggcggtctc
    19021 ctgcctcggg cattccgacg cagtggtggt gcgcgctatc caggagcagc ttgcgcgcct
    19081 gccgttcgcg cacacgtcgt tcttcaccaa cgagccgatg gaagcactcg ccgatgcgct
    19141 cgtggcgcgt gccccggaat cgttgcagaa ggtgtacttc gtgtctggtg gatcggaggc
    19201 gatggaagcg gcgctgaagc tcgcgcgcca gtatttcgtc gagaaaggcg agccgcagcg
    19261 gcggcacgtg atcgggcgcc gccagtccta ccacggcaat acgctcggcg cgctcgccgt
    19321 cggcggcaac cagtggcggc gccggcagtt cgagccgctg ctggtcgatg tgacgcacgt
    19381 atctccctgc tacgcgtatc gcggcaagca agccggcgag accgacgagg cttacggcaa
    19441 gcggctggcc ggcgagctcg agggcgagat ccagcgcctg ggcacgcaat cggtaatcgc
    19501 gttcgtcgcc gagaccgtgg tcggcgctac gctcggcgcg gtaacgccgg tgccgggcta
    19561 tttcaagcgc gtgcgcgagg tgtgcgaccg ctacggcgtg ctgctgatcc tcgacgaagt
    19621 gatgtgcggc atgggtcgct gcggcacctt gtgggccttc gagcatgaag gcgtggtgcc
    19681 ggatctggtg gcgatcgcca agggtctggg cgccggctac cagccggtcg gcgctgtcat
    19741 ggtgagcggc gagatccacg accgcatcgt cgccgggagc ggcttcttcc agcacgggca
    19801 tacctatctg gggcatgctg ctgcgtgcgc cggcgcgctg gcggtgcagc ggcgactgca
    19861 cgaggatggc ctgctcgcgc gcgtggcgcc actcggcgca ctgctcgaac aaaggctgca
    19921 cgcggcgttc gacgcgcacg cgcacgtcgg cgagatccgc ggccgcggtc tgttctgggg
    19981 cctcgagctg gtggccgaga agtcgagcaa gaccccgttc gagcccgggc agcgcgtgca
    20041 cgcgcgcgtg aaaaaggcgg cgctcgatgc cggcctgatg tgctacccaa tgggcggcac
    20101 gctcgatggc gcgcggggcg accacgtctt gcttgcgccg ccattcattg ttgaagaacg
    20161 gcagctcgac gagctggtcg cgaagctcgg cgccgcgatt gacgctgcga tccgcgcaag
    20221 ggttgccgcg tagcagccta gtcggctaag ctaggctggc actctacttg gaggagaccg
    20281 ttatgcgcac actcagcgcg ctcgcagcaa gcctgatcgt cgccggcgct tttgcgacct
    20341 cggcgcaggc gcaacagaaa tacatgacca tcggcacggg cggcgtcacc ggcgtgtatt
    20401 acccggcggg cggcgcagtc tgccggctgg tgaacaagga ccgcgccaag cacggcatcc
    20461 gctgctccgt cgagtcgacc ggcggctcgg tcttcaacat caacaccatc aaggccggcg
    20521 agctcgacat gggcgtcgcg cagtccgacg tgcagttcaa cgcggcgaag ggcaagcagc
    20581 agttcaagga gccgttcggc gagctgcgcg cggtgttctc gctgcacccc gaaccggtaa
    20641 cagtggtggt gcgcaaggaa tcgaacatca acagcttcgc cgacctgaag ggaaagaagt
    20701 tcaacgtcgg caacccgggt tcgggcacgc gcgccacgct cgacgagctg atcggcgcga
    20761 tgggctggaa cctgtcggcc tttgcgctcg cttccgagct gaaggccgac gagcacggcc
    20821 cggcgctgtg cgacggcaag atcgacggct tcttctacct cgtcggccat ccgtcggcga
    20881 acatccagga tccgacgacg gtgtgcggcg cgaagctcgt gtcggtcacc ggctcggccg
    20941 tggacaagct ggtgtcggcc aacccgtact acgcgcacgc caccatcccg ggcggcctgt
    21001 atccgggcaa cccgcaggac acaaagacct acggcgtgct cgccaccgtg gtgtcttcga
    21061 cgcgcgtgcc ggccgacacc gtgtaccagg tggtgaaggc ggttttcgac aacttcgacg
    21121 agttcaagaa gctgcacccc gcgttccagg tgctcaagcc cgagaacatg atcaaggatg
    21181 ggctgacggc gccgctgcat gacggcgcgg tgcgctacta caaggaaaaa ggctggctga
    21241 agtaggcggc ggcttgcgct agcttcacca aagggggcgg aatgcgagtt tcgccccctg
    21301 ttgttttcgg ggagaagacc atggccaaga cggctcccgc accgagcagc gacctgaagc
    21361 agttggtggc cgactccgac accggcgggc gcgcggcggg cggcatcgcc ggcaagctga
    21421 tcttcgcgct tgcgctgggc tggtcgctgt tccagctgtg gtatgcctcg ccgctgccgt
    21481 tcactttcgg ctggggcgtg ttgaacgaca ccgaggcgcg gtcgctgcac ctgggcgtgg
    21541 gtctgctgct ggcgttcctg tgctatccca cgctgcgcac accgtcgcag cgcaaccgca
    21601 tcccctggtt cgactggatc gtgggcctcg ccgcggccgt ggcgggctcc tacttcctgt
    21661 tcttctacgc cgagctggcg acgcgcccgg gccagcccaa cctgcaggac atcgtggtcg
    21721 cctccgcggg catcgtgctg ctgctcgagg cgacgcggcg cgcggtcggg ctgccgatga
    21781 cgatcctggc ggtcatcttt ctcggctaca tcatgctcgg gcagtacctg ccggacgtga
    21841 tcgcgcacaa gggcgcgtcc tgggagcgca tgctggtgca ccagtggctg accaccgaag
    21901 gcgtgtttgg cgtcgcgctc ggcgtgtcgg tgggctatat cttcatcttc gtgctgttcg
    21961 ggtcgctgct cgacaaggcg ggcgccggca actacatgat gcaggtatcg ttcgcgatgc
    22021 tcgggcacct gcgcggcggg ccggccaagg tggccgtggt gtcctccggc ctgaacgggc
    22081 tcatctcggg ctcgtcggtg tcgaacgtgg tgtcgggcgg catcttcact attccgctga
    22141 tgagcaaggc gggctatggc ggcgtgaagg cgggcgcgat cgagactgca tcctcggtca
    22201 acggccagat catgccgccg gtgatgggtg cggccgcgtt cctgatggtc gagtacgtgg
    22261 gcattccata cacggacgtc atcaagcatg cgttcctgcc cgccttgatc tcgtacatcg
    22321 cgctcttcta tatcgtgcat cttgaagcgc tcaagctcgg cctcgagccg acggcggccg
    22381 cgcgcgcgcg cccgttgcac cagaagctga ccgcctgggg cctgggcgtc acgggcacga
    22441 tcgtggtgtg cagcgtgatc tactgggtcg ccgaggccgc gaagaacctg ttcggtcccg
    22501 tggcggcgcc gtgggtgctc ggcctgctgc tcgccgcgct ctacgtctac accgtgtggg
    22561 tggcggcgcg ctgccccgac ctgccgcagg acatcgacgt cgacaacccg atcctgcccg
    22621 acacctggcc tacggtgaag gcgggcctgc atttcctgat cccgatcggc gtgctcatct
    22681 ggtgcctcat gatcgaagag ctgtcgcccg cactctcggc gttctgggcc acggccagcc
    22741 tggtcgcgct gatggcgacg cagcgcccgc tgacggcgtt gttccgcggc aagcgccaga
    22801 tggcgaaggc ggcgcgcatc ggggcggtgg aggtcgtgga ggggctcaac gacggcgcgc
    22861 gcaacatgat ctccattgcc atcgccacgg ccaccgccgg catcatcgtc ggcggcatca
    22921 cgctcacggg cctcgggttc cgcatgaccg acttcgtcga ggtggtgtcg cagggcaatg
    22981 tgttcgcgat gctgctgttc acggcgttcg tgtgcctcgt gctcggcctg ggcgtgccga
    23041 cgacggcgaa ctacattctg gtggcgacgc tgatggcgcc ggtgatcgtg gaactcggcg
    23101 cgcagtcgga tctggtcatt ccgctgatcg cggtgcacct gttcgtcttc tactacggga
    23161 tcatgggcga catcacgccg ccggtgggcc tggcaacgtt cgcggcggct gcgatctcgg
    23221 gcgaagacgc gatcaagacc ggcgtgcagg gcgcggtgta cgcgttgcgc acggtggtgc
    23281 tgccgttcgt gttcatcttc aacccgatgc tgctgctgat cgacgtgcgc agctggtggg
    23341 aggtactgct ggtcgcgttc gccgcgaccg tggcgtcctg cgtcttctcc gcggcgacgc
    23401 tcggctggtt ccagacgcgc tgccgctggt gggagatcgt gttgctgctg gtcggcacgt
    23461 tcgcgctgtt ccgtcccgac tatttcatgg acaagctcta caccccgtac acgcaagtgc
    23521 cggccaagga gatcttccgc gtggcaggcg agctgggcga gaaggagcgg ctggtgctgg
    23581 tcatcaaggg caccaccatc gagggcgagg acgtgcgcaa gaccgtggcg gtgcagctcg
    23641 ccgagccggg cgacggccgc aagcgcctgg ccgctgccgg actcaccatc atggcgctgg
    23701 gcgaggaagt ccgcgtcgcg gccgtgcgct tcggcagccg cgcgcgcaag tccggattcc
    23761 agcagggctt cgaggtggcc gcggtcaagg tgccgaccga ccggccgtcc gagcactggg
    23821 tctacattcc ggcgctggca ttgatcggct tcgtctactt ctggcagcgc cgccgcggcg
    23881 cggctggggg ccggcgccgg cgtccgagct agatggcgcc gaccttctgt ttgcccgccg
    23941 gcacggtgcg gatgatctcg accttggagg cgtagagctc ggcgcgcttg tcgtaaagcg
    24001 gaatcgtggt gccgtcaacg cggttcgaga acagcagctc caaatcgacg tcggcgatca
    24061 cgatctgctc ggtgttggcc acgccttccg cggcgatgcc gtcgcgagaa aatgggtagt
    24121 cggacggggt gaggatgctc gcctgcccat agtgcaggcc gaggccttcg acgggcagcg
    24181 agccgacggt gctcgccatg gcgacataga cctggttctc gatggcgcgc gcatggcagc
    24241 agtagcgcac gcgccagaaa ccctgccggt cgtcggtgct cgaaggcacg aacaggatat
    24301 ccgcccctgc atcgcagacc atgcgcgcga gctcggggaa ctcgacgtcg tagcagacga
    24361 gaatcgcgat atccccgaac tcggtctcga acagacgcag gtgatcgccc ggggtggttt
    24421 tccactgctc tcgctcccag cgcgtccgat gggtcttgtc ctgcgtgtgc atctcgccgc
    24481 gcggcgagaa aaggtacgaa gtgttgtaga gcttgccgtc gcgcagcgtg gggtggctgc
    24541 cgccgattag gtagtagccg ccgcgctcgg cgtgctcgca catgaggtcc tggtagcggg
    24601 cggtgaactg gtcgagcgcg cggatagcgg cgtgcacgtc ctggcgcggc agcgccgaga
    24661 gcagctgcgt ggtgaagatc tcgggcagca gcacgaactg cggccggtag tcccccgccg
    24721 cgcccaggac gaagcgcacc tggcgctcga actcgcgcca gtcgccgatg gggcgcagca
    24781 tgtattggat ggtcgcgatg cgtgctttca caggatcggt cctttaggga ttgcggtggg
    24841 ctggtcagcg cggtagcggt cgttcagcca tacgatgatg ttcgcatgcc cgcaggattc
    24901 cttgtccgag ggcaggtagt cgcgcaccac gccgcaccag tggaagcctt cgctcatctg
    24961 gaacatgagc acggggtctt ccagatcgcc ccagatgaca cgcgcagaat attcttccgg
    25021 cgacatcctg tcggccacca gatggtagtt cggcatgcga ccgcacgaca tgatgcggcg
    25081 caggttgagc gcgcggcaca ggcgccggcg cgccctgtag agcgcgcggc cgatgccgag
    25141 gcgacgcgag cgcgggtcgg caaacacttc ggccccgtag agcgtgcgcg ccgaagcgtc
    25201 gtgcgtggta aacattcctg ctgcagtcac ttcccggtag gtgtggtggg tgccgtagga
    25261 atcccacttc acgatgagcg agcttgccgc cccgacgacg cgctggtcga tgcaagcaac
    25321 gagttggccc tgcggaaaga cgcgcagctg gctctccagc tgctccatgc gccagccgcc
    25381 gatgccgggg tagacgctgt cctggatctc cttgacgcgg gcgagatcgt cggcctgcat
    25441 gtggcgcacg taaggcgtcg gacgcatcgc gcgaattcta ggttagaggc gactcaaacg
    25501 gtcaagctgg cgcgcgccag cagcgcggcc gcgacgctga cgagcgtggc gagcaaaaag
    25561 agcgtcgaga ccgagccttg ctcgagcaga aggccacccc cggcgagccc aatacccacc
    25621 ccggcaagca gcgccgtcgc cgaccacgtg aagccctcgg tggcatgttc cgttttcacg
    25681 gacttcgcga cgagcgttgc ctggatgatc agcgtgggcg tcatcgcgat cccggcgatc
    25741 acgcaccaca gcgcgaacgg gacagtctgg gataccagcg caaggggcgc gatgcccaga
    25801 cccatgactg cgagtgagta ggagaactgg cgagcgagtg cgagatgcca gtggcgggta
    25861 ccgtaggcaa gcgcacctgc ggcgctgcct acgctcatca cgccgagcag caccccggcg
    25921 agcgcgggac ggccggactc ggcagcgtac gccgcgacgc cgatctcgac gaggccgaaa
    25981 accgaggcgt agcaaacgac caggacgagc agaagcgcaa agcgtggctc cgctacgggg
    26041 ccgagaaagg cgccagagaa gtgcgtcgcc ctggtccagc tggcaagcgc acgcgttcgc
    26101 atgaactgca gagacccgag cagcccgcag agggcggccg ccaggacggc ggcagcgggg
    26161 gaaaagaccg cgaccagcag cgccacgagc agcggcccgg cgatgaacac cagctcgacc
    26221 atcagcgcat cgagcgagag cgcggcagtc agcagcgcgt cgtcgcccag cttttggcgc
    26281 aaccacgcgc gctggcaggc ggttaccggc ggaaagctgg cacccgcgag ggcggcgaag
    26341 aggaggccgg ccgcggtcgg cgcacggccg agcagcgccg cgatgagcgc gagcaatgct
    26401 gcgggatata gcaacgcgca ggcggcaagc acgcggcgcg ggcctattct gtcgatcagt
    26461 cggccgagca ggggtgcggc cacggcgagg ccggcgacat acgttgcacc gatcaggcca
    26521 gcctgagcgt acgaaccctc cacgctctgg acgagcagca agatggcaag gccggcgatg
    26581 ccgatgggga tgcggccaat gaatgaactg acgaaggcct gcgggacgtc gggtagcgcc
    26641 agcaaactgg cgtaccgcgc gagggagatc atagaaggcc gcgattctcc catgggatca
    26701 gtgatgctgc agtgcagcat cttcctaagt tgctcttgtt ccacgtggaa catggttagg
    26761 cgtagcggga caagctagtt tcacgtggaa cattcacgta tcatgcggcc tctcccgaag
    26821 caggcccgta tgcagtttcc gagtgaattc gacgtcatcg tggttggcgg tggccacgct
    26881 ggcaccgaag ccgccctcgc cgccgcgcgt atgggctgca gcacgctgct cttgacccat
    26941 aacatcgaga cgctcggaca gatgtcctgc aacccctcca tcgggggtat cggtaagggg
    27001 cacctggtaa aggaagtcga tgccctgggt ggcgctatgg cgctcgcgac cgacgagtcg
    27061 gggatccagt ttcggacgct gaatcggtca aagggcccgg ccgtgcgggc gacacgtgcc
    27121 caggcggacc gcgttcttta ccgacgtgcc attcgcacgc ggctggagaa ccagccgaat
    27181 ctgacgctat ttcagcaggc ggtggacgac attgtgctcg agggcgatcg cgcctgcggc
    27241 gtggtgaccc agctcggcat ccggttccgc ggccgggcgg ttgtactgac tgtgggcacg
    27301 ttcttatcgg gccgtgttca cgtaggtctg gagaactacg cggcggggcg cgccggggat
    27361 ccggccgcag tgacgcttgc agcgcgactg cgggaaatgc atctgccggt gggtcgcctt
    27421 aagaccggga caccgccgcg cctcgatggg cggacgatcg acttcacggc catggaggag
    27481 cagcccgggg acactcccga gccggtgttt tcctttctcg gcgatcggag catgcacccg
    27541 aggcagctgc cgtgctgggt ggcgcacacc aacgagcgca cccatgagtt gattcgcgga
    27601 gcgctggacc gctcgcccat gtacaccggg gtcatccagg gcgtcgggcc acgctattgc
    27661 ccgtcgatcg aggacaagat ccatcgcttc gccgacaagg cgagccatca gatcttcctt
    27721 gagccagagg gtctcgagac gcacgagatc tatcccaatg gcatttccac cagcctgcct
    27781 ttcgacgtgc agttgcagct cgtgcgctcg attcgcgggc tggaacaggc gcatatcctg
    27841 cggccgggtt acgccatcga gtacgactac ttcgacccgc gcaatctgaa gtcgtcgctg
    27901 gaaacgaaga ccgtggccgg actgttcttt gccggtcaga tcaacggcac gactggctac
    27961 gaagaggctg cggcacaggg gctgctggcg gggatcaacg ccggacttcg ggtacgcggc
    28021 ggggcgccgt ggtgcccgcg ccgggacgag gcgtacctgg gcgtgctcgt ggacgacctc
    28081 gtgacgcgag gcgtgtccga gccgtaccgc atgttcacca gccgcgccga gtaccggctg
    28141 atgctgcgcg aggacaacgc cgatctgcgc ctgaccgaag ctggccgcag gctcggggtc
    28201 atcgacgact cgcggtggga tgccttctgc cgcaagcgtg acgccattac ggctgagcag
    28261 gaaaggctgg agaaactgtg ggtcggccca cgggcgatca ccgaagccga ggcaacccgg
    28321 gtgctcggcc agccgctcga gcgcgagtac accttggcat cgctgctgcg ccggcctaat
    28381 gttagttatc gctcacttat gacactagcc aatacgggct ttagtgtcga gagcgaccag
    28441 gtggctgagc aaatcgacat ccaggccaag tatcaaggct atgtggagcg gcagaaggcc
    28501 gaggtggagc gacgtgccca gcatgaggat catcttctgc cgcacgcgct ggattacgcc
    28561 tcggtgcgcg ggctttccaa ggaagtacag cagaagctgc aggaccaccg ccccgaaacg
    28621 gtgggccagg cggcgcgcat ctcaggggtg acaccggcgg cgattgcgtt gttgctggtg
    28681 cacctgaagc gcgtaacaac gctgcagaag aaaagtgcat gacgccggcc gctgcgctgc
    28741 acagcgggct cgccgagctc ggccagttat tggacgacac ggcgcagcag aagctgatcg
    28801 ccttcatcga actgctggcg aagtggaaca agacctataa cctgaccgcg atccgcgatc
    28861 cgctgcagat gatcagccac cacttgctcg attccctcgc cgtgcgcccg caccttccgg
    28921 ccggcgccct tgccgacgtg ggctcgggcg gcggcctgcc gggcattccg ctcgccatcg
    28981 ccgagccgca gcgccgcgtg acgctgaacg atgccaatca caagaaggcc gcgtttctgc
    29041 agcaggcggt catcgagctc aagttggcga acgccgatgt acacacggga agggtgcagg
    29101 cttggcaccc gtcgagtccg ttcgcgtgcg tgatcgcgcg aggctacgcc gagctcgcgc
    29161 aattcatcgc cagctgccgc cacctagtga gcacgggcgg cgcgcttgcc gccatgaaag
    29221 gtgcctaccc gaaggaagag ctgagccggg tgccgcagga cgtcgagtgc agggaagtgt
    29281 ggcgcctgca ggtgccgtcg ctcgatgcgg agcgccacct cgtgctgtgc cggtgtgcgc
    29341 catgagcgaa acgcgcatcg tcgccgtcgt gaatcaaaaa ggcggggtcg gcaagaccac
    29401 caccagcgtg aatcttgcag cggcgctggc gcgcgcggga cagcgcacgc tgctggtgga
    29461 cctggacccc caaggcaacg ccaccatggg cagcggcatc gacaagcgtg ccgcatcgcg
    29521 caccgtgtat cacgtgctgc ttggcttggg cgagatcgcc aagatccggc tgcgctccga
    29581 gcagggcggc tacgacctgc tgccggcaaa ccgtgagctc gcgggagccg aagtcgagct
    29641 ggtggagctg aacaaccgcg aaggccggct caaggctgcg ctcgccagcg tgcgcgacga
    29701 gtacgacttc gtgttcatcg attgcccgcc ttcgctgtcg ctgctcacgc tgaacggcct
    29761 ggtcgcggcg caatcggtgc tgatcccgat gcaatgcgag tactacgcgc tcgaggggct
    29821 gtccgacctc gtgggcacca tcaagcgcgt gcgcgccaac ctgaatccgc agctggagat
    29881 cacggggctc gtgcgcacca tgtacgacgc gcgcaatacg ctcgcacagc aggtgtcggc
    29941 gcagctggaa acgcatttcg gcgacaaggt gtaccgcagc ctggtgccgc gcaacgtgcg
    30001 cctggcagag gcgccgagct acggcatccc ggccgtggtc tgggatccgg agtccaaggg
    30061 cgcgcaggcc tacctggcgc tcgccgacga gatgctcgag cgtgagcgtg ccggcctgcc
    30121 ggcggcggcg gcatgaacca gaagcgcaag ggactggggc gcggccttga cgcgctgctc
    30181 ggcggcgacg aggcgccgcg cgacaggctc gccacactgc cggtggcgcg gatccgtccg
    30241 ggccgctatc agccgcgtac ccgcatggac caggaggcgc tcgcggagct cgcctcgtcg
    30301 atccagtcgc agggcctgat gcagccggtg ctggtgcgac cggtcgatcg cgagcgctac
    30361 gagctcattg ccggcgagcg gcgctggcgc gctgcgcaga tggccgggct gcaggaaatt
    30421 ccggcgctgg tgcgcgaggt ggccgacgaa tccgcgctcg ccatgtcgct gatcgagaac
    30481 atccagcgcg agaacctcaa cccgatggaa gaggccgcgg gcctgcagcg tctggtggac
    30541 gaattccgca tgacgcatga acaggccgcc gacgcagtag gccgctcgcg cagcgccacg
    30601 accaacttgc tgcgcctgct gcgcctggca aagccggtgc agggcatggt gatggagggc
    30661 gtgctggaga tgggccatgc gcgcgcgctg ctgtcgctgg acggcgcacg ccagatcgag
    30721 gccgccaacc gcatcgccgc gcgcggcctg tcggtgcgcg aggccgaagc cctggtcaac
    30781 aagatgcagc gcgggcagac agcgcgccgc gcgcgcaaga ccgaccgcga cttggcgcgg
    30841 ctcgaagagg aggtctcggg ccgtctgggc acgaccgtgg agatccgcgc cggaggcaag
    30901 ggccgcggca aggtaatcgt gcattactcc gggctcgacc atctcgacca gctgctgaag
    30961 aagctgcgat gaaacagcgg ccatgctgcg ccgcaacgcg ttgaccagtg ttggcgcaag
    31021 cagataaaat cccacggctt tgcgggatgc gcagcgcgtg aacctgagcc tgggcagcct
    31081 gcaacggccg atccgcactg tcctgcggtg gcagttggcc gccaccgcag cactcacgct
    31141 cctggcgggg gtcgtggccg gcgccgaggg tgccctgtcc gcggctctcg gtggcggggt
    31201 gagtgtcgtc gccggcggcg tatccgcctg ggtggcggcg aggggcaagg cgcgatccgc
    31261 gggggaggtc ctgttgggtg cgttgctggc cgagggcgtc aagatcggcc tgctcgtgct
    31321 cctgttgtgg ctggtgctgg cgacctacaa gggcgtcgtc gcgccagtgt tcctcggatc
    31381 gttcgtggca acggtcctga tatttggcat ggcttttttt gtgcgcgaga acgactgaaa
    31441 gaatggctgc cggcaaagac ttcaacgcct cggattacat ccagcaccac ctgaccttcc
    31501 tcaccaagcc ggtgggcgac ggcgggttct gggcgctgaa cgtcgacacg ctcgtcacct
    31561 cggtgctgat cggcgtgctc gccatgggcg cgatctggtg ggtggtgcgt ggcgcgaccg
    31621 cgggcgtgcc caaccggcgc caggcactgg tcgagatgct gatcgagttc atcgacgacc
    31681 aggtgaagag cattttccac cacggcgacc gcaacacgtt cgtggcaccg gctgcactca
    31741 ccgtgttcgt gtgggtgctg gtgatgaacg cgatggactt tctgcccatc gacatcgtcg
    31801 tcggtccgct ggagtggatg gggctgcacg cgtggcgccc ggtgcccacc gccgacatca
    31861 acaccacgtt cgccttggcg ctgtcggtat ggctgctgat gatctggttc tcgattgcat
    31921 ccaagggttt gttcggctgg atccacgaac tgttctgcgc gccgttcggc aagaatcccc
    31981 tgctgtggcc cgccaacctg ctgttcaact tcattgagta cgtgtccaag cccctgtcgc
    32041 actcactgcg gctgttcggc aacatgtacg caggcgagat catcttcctg ctgctctggc
    32101 tgctcggcgc gaccggcctg ttcggcacgc tgatcgccac cgtgctcggc ctgggctggg
    32161 ccatcttcca cattctgatc gtggtgctgc aggcctacat cttcatgatg ctgacggtgg
    32221 tgtacatcgc catggcccac gagcatcact gaacccgact caaccgacct tcccgcttag
    32281 aaaggaaagc accatggaga aactgcaaat gctggcgatg attcaggcct acacgggcat
    32341 cggcatcggc ctgatgatcg gcgtcggcgc ggccggcgcg tgtataggcg tgggcatcat
    32401 gtgcagccgc ttcctcgagg ccgccgcgcg ccagcccgag ctcacgaact cgctgcaggt
    32461 gaaggtgttc ctgctgctcg gcctgatcga cgcctcgttc atcatcggcg tcggcctggc
    32521 gatgatgttc gcgttcgcca acccgctgct cgccgtcatt cagtaggcgc gaccgcccgc
    32581 agggagccgt tatgagcttc aatctgacgc tgatcgccca ggcgctcacc ttcgcactgt
    32641 tcatctggtt cacggtgaag gtggtctggc cgtacatgct gcgtgccatc gaggcacggc
    32701 agaagaccat cgccgacggc ctggcggccg ccgagcaggg gcacaagtcg ctcgagtcct
    32761 ccaaacgcca cgccgaggac gccatcagtc aggcgcgcaa tcgctcgagc gaaatcctgg
    32821 cgcaggccga gaagcgcgcc gtgcagatgg tcgaagaggc gaagaacgcc gcgcgtgctg
    32881 aaagcgagcg cgagaaggcc gccgccaagg ccgagatcga gcaggaagcc tcgcgcgcgc
    32941 gcgagcaact gcgcgaccag gtggccgcgc tcgctgtggc cggcgcggag aagattctgc
    33001 gccgcgaagt cgacgcgaag gcgcacgccg agttgcttga gtccatcaag aggcagctgt
    33061 agcagcatgg ccgagcccag caccatcgcg cgaccctacg cccaggcggt gttcgcgctc
    33121 gctgacggcg ccggcaagct cgccgagtgg tcggcggcgc ttgccaacct tgccgcggtg
    33181 gccgaggacg ggcgcgtacg cgaggcggtg tccgatccga accggtccgc ggcgcaggcc
    33241 gcgggcctgc tgagctcgat actggcgggc aagctctcgg gcgacgcggc gaacctggtg
    33301 cgcgtgcttg ccgagaacgg ccgcctcgac gtgctgcagg aaatccgcac gcagtacgag
    33361 gcgctgaaga acgagcgcga aggcgtgatc gaggccgagg tgatctcggc gttcgagatg
    33421 aacgaggcgc aggtcgccga tctggtggcg cgcctggaga acaagaccgg gcgcaaggtc
    33481 aagatgcgcg ttagcgtcga tcagaacctg atcggcggcg tcaaggtggt gctcggcgac
    33541 aaggtcatcg atggcaccgc gcgcgcgcag ctcggcgcgc tcgaggccgc attgaaagct
    33601 taaggagcag gcatgcaact caacccgtcc gaaatctcgg aactgatcaa gagccgcatc
    33661 cagaacctcg atgcgagcgc gcagatgcgc acgcagggca ccgtggtgtc ggtgaccgat
    33721 ggcatctgcc gcgtccacgg gctgtcggac gtgatgcagg gcgagatgct ggaattcccg
    33781 aagagcacct tcggcctggc gctcaacctc gagcgcgact cggtgggcgc ggtgattcta
    33841 ggtgagtatg agcacatcac cgagggcgac accgtgaagt gcaccggcaa gatcctgtcg
    33901 gtgccggtgg gcccggagct cctcggtcgt gtggtcaact cgctcggcca gccgatcgac
    33961 ggcaagggcc cggtgaagac ccggatgacg gacgtcatcg aaaaggtcgc gcccggcgtg
    34021 atcgcgcgcc agtcggtgtc gcagccggtg cagacgggcc tgaaggcaat cgacgccatg
    34081 gtgccgatcg gacgcggcca gagggaactg atcatcggcg accggcagac cggcaagacc
    34141 gcggtggcgg tggacacgat catcaaccag aagggcaagg acctgttctg cgtgtacgtg
    34201 gcgatcgggc agaaggcctc caccatcgcc aacgttgtgc gcaagctcga agagcacggc
    34261 gccatggagt acaccatcgt ggtcgccgcg tcggcctccg aatcggcggc catgcagtac
    34321 atcgcgccgt attccggctg caccatgggc gagtacttcc gcgaccgcgg ccaggacgcg
    34381 cttatcatct acgacgacct gaccaagcag gcctgggcgt accgccagat ctcgctgctg
    34441 ctgcgccgtc cgcccgggcg cgaggcatat cccggcgacg tgttctacct gcactcgcgt
    34501 ctgctggagc gcgcggcgcg cgtgaatccc gagtacgtgg agaaattcac caagggcgag
    34561 gtcaagggca agaccggttc gctcaccgcg ctgccggtga tcgagacgca ggccggcgac
    34621 gtgaccgcgt tcgtgccgac caacgtgatc tcgattaccg acggccagat cttcctcgaa
    34681 tcggacctgt tcaacgccgg tattcgtccg gcgatcaacg ccggcctgtc ggtatcgcga
    34741 gtcggcggcg ccgcgcagac cccgatcatc aagaagctcg gcggcggtgt gcgactcgcg
    34801 ctggcccagt accgcgagct ggcggctttc tcgcagttcg cttccgatct cgacgaggct
    34861 acccgcaagc agctcgaacg cggccagcgg gtaaccgagc tgatgaagca gcgccagtat
    34921 tcgccgctgt cggtcgccga aatgggcgtt tcgctgtatg ccgccaacga gggtttcctc
    34981 gacgacgtca atgtcaacaa ggtggtcagt ttcgaggatg caatgctcgc gcatattcgc
    35041 tccaacaacc aggacatgct cgacaagatc aacgaatccg gcgactacaa cgacgagatc
    35101 gcggccgaga tcaggaaggc gatcgaagac ttcaaggcca gcggcgtcta ctaggggatt
    35161 tttcgatggc tggcggaaaa gaaatcaaga ccaagatcgc gagtgtgcag aacactcaga
    35221 agatcaccgc ggctatggaa atggtcgcgg ccagcaagat gcgcaaggcc caggaccaga
    35281 tgttcgccac ccggccctac gccgagcgca tcgccgaggt ggtcagtcat gtggcgcgcg
    35341 cgcacgccga gtacaagcat ccgttcctga tcgaccgcga aatcaagcgt gccggcatca
    35401 tcgcgatttc caccgatcgc ggtttgtgcg gcggtctcaa caccaacatg ttcaagaaag
    35461 cgctgaacca catcggcgaa ctcgacgaac agggtatcga gatcga
//
LOCUS       DBNHKE010000230         3997 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_202748, whole genome shotgun sequence.
ACCESSION   DBNHKE010000230 DBNHKE010000000
VERSION     DBNHKE010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_202748"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1201
                     /locus_tag="AC16O9_15780"
     CDS             68..1201
                     /locus_tag="AC16O9_15780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518116.1"
                     /translation="MTLRLLIVLTVLAHMGFVGSRVTVSLNAIAFGATPLTVGVLMSL
                     YAALPMLLAVHAGRRIDRAGVAGPLLAGLLAVAGGVLLPALWPSLAALFVAAMVIGTG
                     FLFVHVALNNAVGALAAPELRAASFAWLALGFSVGGFFGPLAAGFAIDGLGHRLAFLV
                     LAACPALAAAVLWARRRRLPAHAVPKAETGEHRLADLLRDPRLRNAFIASAVIAMGWD
                     LYAFVVPIYGTRIGLSASLIGIVMGSFSAATFTVRLALPALARRVREWPMVMAAMVIA
                     GLSYALFPLAESVAPLMALSFALGLGLGCAQPFIMSLLYAASPPGRQGEVVGIRTTML
                     SGSHAVLPLAMGALGAALGMAPVFWSMALVLLAGGGFAWRRVK"
     gene            1210..2211
                     /locus_tag="AC16O9_15785"
     CDS             1210..2211
                     /locus_tag="AC16O9_15785"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR6518117.1"
                     /translation="MRHVALSQFLIQRQHAGKINADLRLLMETVARACKAISTRVNKG
                     ALADGHGATGGENVQGEAVQKLDMLSNEILLEANEWGGNLAALASEEMELPHPIPTHF
                     PKGEYLLLFDPLDGSSNINVNISVGTIFSVLRCPKMADGSLCEANEAAFLQPGRRQVA
                     AGFAVYGPTTVLVLTVGDGAHAFTLDRETYTFVLTHPDIRIPADTAEFAINISNQRRW
                     EPPVKRYIEECLAGKDGPRGKDFNMRWVASMVADVFRVLSRGGIFMYPRDAKNTNGRL
                     RLMYEANPMAYIVEQAGGAATDGRTRILELQPTGLHQRVAVILGSKNEVDVVTSYHK"
     gene            complement(2403..3134)
                     /locus_tag="AC16O9_15790"
     CDS             complement(2403..3134)
                     /locus_tag="AC16O9_15790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HIR6518118.1"
                     /translation="MFWVKKEDEPKFNEAVDTLSKIFGFTFAGDMLITFNRNMGFFED
                     ARFMEAFNGNVRFPVEKTLIWRLHVLCWNALNSLRVEGDFVECGVFQGFCSAVAARYL
                     DFGSVDKQWYLYDTFSGIPPDQLNPGHQNSPDFEQPGLYEGVVQRFAGYRNVHVVRGR
                     VPDVLHERAPEKVAFMHIDMNSAAAELAALEFFWERLSPGAAIVLDDYGWRGYRAQKL
                     AEDPFFAARGRQVLELPTGQGLVLV"
     gene            complement(3208..3585)
                     /locus_tag="AC16O9_15795"
     CDS             complement(3208..3585)
                     /locus_tag="AC16O9_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HIR6518119.1"
                     /translation="MPLWIRHLSLTAAPRRQYPDLEISVQRRDTMAKKKAKKAGKKRK
                     PNAAFMKAMNPSATLSAVIGSSAMPRTEVTKKIWGYIKRNGLQDKKNRRMINADDKLK
                     AVFGGKSQVSMFEMTKLVSKHLK"
     gene            3761..>3997
                     /locus_tag="AC16O9_15800"
     CDS             3761..>3997
                     /locus_tag="AC16O9_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR6518120.1"
                     /translation="MNIADLRQEYMRMGLAEAQAHADPLVQFERWLQDALAAELPLAN
                     AMTLATVAASGAPTARVVLLKGLEQGGFVFYTNYR"
BASE COUNT          593 a         1378 c         1318 g          708 t
ORIGIN      
        1 tcggcatcgc cgtcacctgc ctgggcgtcg cgctggtgac cgcgcgcggc aggcggcagg
       61 ccgcggcatg acgctgcgcc tgctcatcgt cctcaccgtg ctcgcgcaca tggggttcgt
      121 cggcagccgc gtgacggtgt cgctcaacgc catcgccttc ggcgcgaccc cgctcaccgt
      181 cggcgtgctg atgtcgctgt acgccgcgct gccgatgctg cttgcggtgc acgccgggcg
      241 gcgcatcgac cgcgccggcg tggcggggcc gctcttggcg gggcttctcg cggtggcggg
      301 cggggtcctg ctgccggcgc tgtggccgag cctcgccgcg ctgttcgtcg ccgcgatggt
      361 gatcggcacc ggctttcttt tcgtgcacgt ggcgctgaac aacgccgtcg gggcgctggc
      421 cgcgcccgag ctgcgcgcgg cgagcttcgc ctggctggcg cttggctttt cggtgggcgg
      481 cttcttcgga ccgctcgccg ccggcttcgc catcgacggc ctcgggcacc gcctcgcctt
      541 cctggtgctc gccgcgtgtc cggcgctcgc cgccgccgtg ctgtgggcgc ggcgccggcg
      601 cctgccggcg catgccgtgc cgaaggcgga aaccggcgag caccgcctgg ccgacctgct
      661 gcgcgatccg cggctgcgca acgccttcat cgcgagtgcc gtgatagcga tgggctggga
      721 cctgtacgcc ttcgtcgtgc cgatctacgg cacgcgcatc gggctttcgg cttcgctgat
      781 cggcatcgtg atgggcagct tctcggcggc gaccttcacc gtgcgcctgg cgctgcccgc
      841 gctggcgcgg cgcgtgcgcg agtggccgat ggtgatggcg gcgatggtga tcgccggcct
      901 gtcctatgcg ctgtttcctt tggccgagag cgtggccccg ctgatggcgc tctccttcgc
      961 gctcggcctc ggcctgggct gcgcgcagcc cttcatcatg tcgctgctct atgccgcatc
     1021 gccccccggg cgccagggcg aggtggtcgg catccgcacc acgatgctga gcggcagcca
     1081 cgccgtgctg ccgctcgcca tgggcgcgct cggcgccgcg ctcggcatgg caccggtgtt
     1141 ctggtcgatg gcgctcgtgc tgcttgccgg cggcggtttt gcgtggcggc gggtaaaatg
     1201 agctacgcca tgcgccacgt cgccctctcg cagttcctga ttcaacgcca gcacgccggc
     1261 aagatcaacg ccgacctgcg cctgctgatg gagaccgtgg cgcgcgcctg caaggcgatc
     1321 agcacgcgcg tcaacaaggg cgcgctcgcc gacgggcacg gcgcgaccgg cggcgagaac
     1381 gtgcagggcg aggcggtgca gaagctcgac atgctgtcca acgagatcct gctggaggcg
     1441 aacgaatggg gcggcaacct ggccgcgctc gcttccgagg aaatggagct gccgcatccg
     1501 atccccacgc acttcccgaa gggtgaatac ctgctgctgt tcgacccgct cgacggctcg
     1561 tccaacatca acgtcaatat ctcggtcggc accatcttct cggtgctgcg ctgcccgaaa
     1621 atggccgacg gcagtttgtg cgaggcgaac gaggccgcct tcctgcagcc cgggcgcagg
     1681 caagtggcgg cgggcttcgc ggtctacggg ccgacgaccg tgctagtgct caccgtcggc
     1741 gacggcgcgc acgcgttcac gctggatcgg gagacctaca ccttcgtcct cacgcacccg
     1801 gacatccgca tcccggccga taccgccgag ttcgccatca acatctccaa ccagcggcgc
     1861 tgggagccac cggtgaagcg ctacatcgaa gagtgcctcg ccggcaagga cggcccgcgc
     1921 ggcaaggact tcaacatgcg ctgggtggcc tcgatggtcg ccgacgtgtt ccgcgtgctc
     1981 tcgcggggcg gcatcttcat gtacccgcgc gacgcgaaga acaccaacgg gcggttgcgc
     2041 ctgatgtacg aggcgaaccc gatggcctac atcgtcgagc aggcgggcgg cgcggccacc
     2101 gacggccgca cgcgcatcct ggagctccag cccaccggcc tgcaccagcg cgtcgcggtg
     2161 atcctcggct ccaagaacga ggtcgacgtc gtgacctcgt accataaata gggacagacc
     2221 tcattttccc tgtttgcgcg acggaccaag cgtaggcgcg ccgccgtcgc cgttcaaaac
     2281 gatctcggct tttctggctg gaagatttac ccagccagaa aagccgagac catagagaac
     2341 cgaacgcagg cttgggcggt tgcgcgtcgc cggcctcaaa gaaaaatgag gtctgtcccc
     2401 atttacacga gcaccagccc ctgccccgtc ggcagctcga gcacctggcg gccgcgggcg
     2461 gcgaagaacg ggtcttcggc gagtttctgc gcgcggtagc cgcgccagcc gtagtcgtcg
     2521 agcacgatcg cggcgccggg cgacaggcgc tcccagaaga actccagcgc ggccagctcg
     2581 gcggcggcgg agttcatgtc gatgtgcatg aacgccactt tctcgggcgc gcgttcatgc
     2641 agcacatcgg gcacccggcc ccggacgacg tgcacgttgc ggtagcccgc gaagcgctgc
     2701 accactccct cgtacaggcc gggctgctcg aagtccggcg agttctggtg cccgggattg
     2761 agctggtcgg gcgggatccc gctaaaggtg tcatagaggt accactgctt gtcgacgctg
     2821 ccgaagtcga ggtagcgcgc cgcgaccgcg ctgcaaaagc cctggaacac gccgcactcc
     2881 acgaagtcgc cttcgacgcg caggctgttg agcgcgttcc agcacagcac gtgcaggcgc
     2941 cagatcaggg tcttctcgac gggaaacctg acattgccgt tgaacgcctc catgaaccgg
     3001 gcgtcttcga agaaccccat gttgcgattg aaggtgatca gcatgtcgcc ggcaaacgtg
     3061 aagccgaaga tcttggaaag cgtatccacc gcctcgttga acttcggctc gtcttccttc
     3121 ttcacccaga acatccgcgc ctcccaggaa tggaaaaggc cgggcgcttg cgcggcccgg
     3181 ccttcccgta actccgcctg aaataggtta cttgaggtgc ttgctgacca gcttggtcat
     3241 ctcgaacatc gacacctgcg acttgccgcc gaatacggcc ttcagcttgt cgtcggcatt
     3301 gatcatgcgg cggttcttct tgtcctgcag cccattgcgc ttgatgtagc cccagatctt
     3361 cttggtcact tcggtgcgcg gcatggcgct cgagccgatg acggccgaga gcgtcgcgct
     3421 cggattcatc gccttcatga acgctgcatt cggcttgcgc ttcttgcccg ccttcttcgc
     3481 cttctttttc gccatcgtgt ctctcctctg aacggaaatc tcgagatccg gatattgccg
     3541 ccgcggtgcg gcggtgagcg aaagatgccg aatccagagg ggcatgtcaa tgaccacgag
     3601 ggagtacggc gcgattttcc cctgcgcctg ccgcgcgtcg cggcgcatgt cgctcattgc
     3661 ggacacgcgc gcgcacttga cgcgcgcggc atggcccggc cgtcgccgcc gagagcagca
     3721 acccgctgat ttccgccttc attcttatag aatggccgcc atgaacatcg ccgacctgcg
     3781 ccaggaatac atgcgcatgg gccttgcgga ggcgcaggcg catgccgatc ccctggtgca
     3841 gttcgagcgc tggctccagg acgcgctcgc cgccgagctg ccgctcgcca acgccatgac
     3901 gctggcgacc gtcgccgcct cgggcgcgcc cacggcgcgc gtcgtgctgc tgaagggcct
     3961 ggaacagggc ggcttcgtgt tctatacgaa ctaccgc
//
LOCUS       DBNHKE010000231        10962 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_520487, whole genome shotgun sequence.
ACCESSION   DBNHKE010000231 DBNHKE010000000
VERSION     DBNHKE010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_520487"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..2782)
                     /gene="acnA"
                     /locus_tag="AC16O9_15805"
     CDS             complement(92..2782)
                     /gene="acnA"
                     /locus_tag="AC16O9_15805"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR6518121.1"
                     /translation="MPHNLHDTLQPLKGGKFYSLPKLGQALGVNIGRLPLSIRIVLES
                     VLRNCDGKKVTEEHVKQLAGWQAHAARTAEIPFVLARIVLQDFTGVPLLCDLAAMRGV
                     AKKMGRNPKVIEPLVPVDLVVDHSVQIDHYGTKNALDLNMKLEFRRNEERYQFLKWGM
                     QAFDTFKVVPPGIGIVHQVNLEYLARGVHRSKDGVFYPDTLVGTDSHTTMINGIGVVG
                     WGVGGIEAEAGMLGQPVYILTPDVVGVHLKGRLHPACTATDLVLTVTEMLRNAKVVNK
                     FVEFFGEGTASLSVTDRATIGNMAPEYGATMGFFPVDEKTLEYFRGTGRSDAEIDAFA
                     AYFKAQGMFGVPKVGAIDYSQTLELDLASVMPSLAGPKRPQDRINLERVKSTFTSLYS
                     RPAAENGFGRKQEDLAKVHRAFDGTEMRNGDVLIAAITSCTNTSNPGVLLAAGLVAKK
                     AVELGLSVKPHVKTSLAPGSRVVTEYLTKAGLMPYLEKLGFNLAAYGCTTCIGNAGPL
                     AQPIEEAIVKNDLVCAAVLSGNRNFEARIHPNIRANFLASPPLVVAYAIAGTMLKDFK
                     TEPLGKSKDGRDVYIGHIWPSADEVKALLKLAMDAPTFRRLYANLGDANPMWKKISAP
                     SGQVYSWPTSTYIAEPPFFAEFDMQPNLPGEVRGAKVLGIFGDSVTTDHISPAGSIKP
                     TSPAGIYLQEHDVAVADFNSYGARRGNHEVMMRGTFANVRIKNLMMGGKEGGLTKFQG
                     QEMPIYDAAMRYIAEGTPTVVFGGEEYGTGSSRDWAAKGTQLLGVKAVIVRSFERIHR
                     SNLVGMGVLPLQFTGSDSAQSLGLKGDETIDVLGLDDIRPQQELTLVITRAGGAKQEV
                     RVKSRIDTPIEVDYFKHGGILPYVLRELISKAGPSAAKAA"
     gene            complement(2772..2984)
                     /locus_tag="AC16O9_15810"
     CDS             complement(2772..2984)
                     /locus_tag="AC16O9_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518122.1"
                     /translation="MSAVAELRGRIAELSLEIRRYPTPIARCDEQLTQLLAQRARRMA
                     QLDKVTRDGPCDPVGHGTNDGGTHAA"
     gene            complement(2981..3406)
                     /locus_tag="AC16O9_15815"
     CDS             complement(2981..3406)
                     /locus_tag="AC16O9_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518123.1"
                     /translation="MTRLLAAAAAALLAGCTSTYSLTLMPRDSGQIYYGEATGQTGGE
                     MRLRVTIGERTYEGTWVVSDPGPSTGFVVGGVFGTRRSGVGTSVIVDNAPGSDAKALL
                     RSADGKGLRCDFKGVTGAAGSGTCRDDQGLTYDVQLRRT"
     gene            complement(3406..4833)
                     /locus_tag="AC16O9_15820"
     CDS             complement(3406..4833)
                     /locus_tag="AC16O9_15820"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HIR6518124.1"
                     /translation="MSAHISNVRPKPDKVLKLIADYATKFQVKRAEAYDTARYCLMDT
                     LGCGFEALEYPACTKLLGPIDPETVTPRGAKVPGTKFQLDPVQAAFSLGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNGQPLTMKDVLTAMIKAHEIQGVIALENSFN
                     RVGLDHVVLVKVASTAVVAHMLGCTYDQVVNAVSQAWLDGQSLRTYRHAPNTGSRKSW
                     AAGDATSRAVRLALISKTGEMGYPSVLTAKTWGFYDVLFKGKPFKFQRGFGSYVMENV
                     LFKISFPAEFHSQTAVECAMQLHALVKDRIKDIKRIKIRTHEAAIRIIDKKGPLHNPA
                     DRDHCIQYMVAVPLIFGRLTAADYEDGVARDPRIDALRAKIVCVEDKRFTRDYHDPKK
                     RSIANALTVEFKDGSKLKEVVCEYPIGHKRRRKEGMPVLVEKFKTNLARRFPRPQQQA
                     ILELCLDAPRLDATPVRDFVDMMVA"
     gene            complement(4833..5036)
                     /locus_tag="AC16O9_15825"
     CDS             complement(4833..5036)
                     /locus_tag="AC16O9_15825"
                     /inference="COORDINATES: protein motif:HMM:NF023369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HIR6518125.1"
                     /translation="MKAYESDLTRFMREFLAAHPEEIESQKIGRAAWWDKTPRTRGAP
                     SPARPAPKAGGNEYTFAPLSENK"
     gene            complement(5033..6184)
                     /gene="prpC"
                     /locus_tag="AC16O9_15830"
     CDS             complement(5033..6184)
                     /gene="prpC"
                     /locus_tag="AC16O9_15830"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036440 - citrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate synthase/citrate
                     synthase"
                     /protein_id="HIR6518126.1"
                     /translation="MNSSPNAPKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGYDIL
                     DFADAAEFEEVAYLLVHEKLPTTQELAAYKAKLKKLRGLPAPLRTALEQMPKSAHPMD
                     VLRTGCSVLGTLEPEAPAHSVEGARAIADRLMASFGSMLLYWHHFAHGGKRIETATDD
                     DSIGGHYLHLLHGKPPSPSHVRAMHTSLVLYAEHEFNASTFTCRVITGTGSDMYSAIA
                     GGIGALRGPKHGGANEVSDEIQKRYRSADEAESDIRRRVAAKEVVIGFGHPVYTIADP
                     RNKLIKAVAERLSREAKDMTQFEIADRIESVMAAEKKMFANLDWFSAVSYSKMGVPTL
                     HFTPLFVISRTSGWAAHVIEQRIDGKIIRPSANYTGPENRQWVPLKDRK"
     gene            complement(6159..7034)
                     /gene="prpB"
                     /locus_tag="AC16O9_15835"
     CDS             complement(6159..7034)
                     /gene="prpB"
                     /locus_tag="AC16O9_15835"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HIR6518127.1"
                     /translation="MSPGARFRQALKEEQPLQVIGAICAYHARLAQRTGYKAIYLSGG
                     GVAAGSLGMPDLGISNLDDVLIDVRRITDVCDLPLLVDCDTGFGASAFNVGRTVRSLI
                     KAGAAATHIEDQVGAKRCGHRPGKELVSAEEMCDRIKAAADARTDRAFVIMARTDGLA
                     GEGLEAALARAVKYAEAGADMIFPEAVTELAQYRKFVDAVKVPVLANITEFGKTPLFT
                     LDELRAAGVAIALYPLSAFRAMNKAALAVYEALRREGTQKDVVAMMQTRDELYDYLDY
                     HAYEKKLDELFAQRS"
     gene            complement(7031..8026)
                     /locus_tag="AC16O9_15840"
     CDS             complement(7031..8026)
                     /locus_tag="AC16O9_15840"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR6518128.1"
                     /translation="MLKAPVRVAVTGAAGQIGYSLLFRIASGDMLGTDQPVILQLLEI
                     PDEKAQKALKGVMMELDDCAFPLLAGMQAASDPMQGLKDADIALLVGARPRGPGMERK
                     DLLEANGKIFAPQGQALDKVAKRDVRVLVVGNPANTNCLIAMKNAKSLKPTQFTAMMR
                     LDHNRATSQIAHKIGKPNASVKKVTVWGNHSATQYPDLFQAECEGKKVWPMINDQAWL
                     EGTFIPTVQKRGAAIIEARGLSSAASAANAAIDHMRSWVLGTPAGDWVSMGVPSDGSY
                     GIAEGVLYGYPVTCKDGRYEIVKGIEVSEFSRKRMEATLKELHEERDGVKSLLGA"
     gene            8169..8570
                     /gene="sdhC"
                     /locus_tag="AC16O9_15845"
     CDS             8169..8570
                     /gene="sdhC"
                     /locus_tag="AC16O9_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR6518129.1"
                     /translation="MANVTIRKPRPKYLSLTALLFEIRLPLPGWVSILHRISGLLLFL
                     AVVWLLFMLDRSLASEADFEAIRRYVGLLPVKLSLLVLIWAFCHHFCAGIRFLLLDLD
                     RGVDKATARFTSWITLAASLALTAWLGAKLW"
     gene            8564..8908
                     /gene="sdhD"
                     /locus_tag="AC16O9_15850"
     CDS             8564..8908
                     /gene="sdhD"
                     /locus_tag="AC16O9_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR6518130.1"
                     /translation="MVDRVVTGAHYGLRDWLAQRVTAAVMAVYTVIMLAVLIGAAPIG
                     YAVWKDLFTQGWMRVATLLFAASLAWHAWVGMRDILMDYVKPTGARLTLQVGVLLVIA
                     AYLAWTIQILWR"
     gene            8913..10688
                     /gene="sdhA"
                     /locus_tag="AC16O9_15855"
     CDS             8913..10688
                     /gene="sdhA"
                     /locus_tag="AC16O9_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR6518131.1"
                     /translation="MSDLPVRRFDAVVVGAGGSGMRAALELARADLKVAVLTKVFPTR
                     SHTVAAQGGIAAPLANSTEDNWHWHMYDTVKGSDYLGDQDAIEYMCRRATEVVVELEH
                     MGMPFDRLENGKIYQRPFGGHTQDYGSAKMAMRACAAADRTGHAMLHTLYQQNVRANT
                     QFFVEWMALDLVRDPQDGAVIGVTALEMETGEVTLFQAKAILLATGGAGRIFAASTNA
                     FINTGDGLGMAARAGLPLQDMEFWQFHPTGVYGAGVLITEGVRGEGGYLLNKDGERFM
                     ERYAPNAKDLASRDVVSRAMTTEIKEGRGCGPEADHVLLKLDHLGPEVINQRLPGIRE
                     IAIKFANADPVREPVPVVPTVHYQMGGVPANYMGQVVAPEGASQEAIVPGLYAAGECS
                     CVSVHGANRLGTNSLLDLLVFGKSSGEQMAKDIRALPRPHRNLPRDSGEASRARLARL
                     DAARGGERVAEVAAELRRVMQAHCGVFRFPEDLAAGVEKMTQIARRAANVAIHDRSKV
                     FNTARIEALELDNLVQTAMSTVVSAQARKESRGAQSRADFPERDDAHWMKHTLWYKEG
                     NRLAYKPVHLKALTVESFEPKVRTY"
     gene            10698..>10962
                     /locus_tag="AC16O9_15860"
     CDS             10698..>10962
                     /locus_tag="AC16O9_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6518132.1"
                     /translation="MKLSIYRYDPDKDAKPYMQELEVTLEHSDRMLLDALVKAKAQDD
                     SLAFRRSCREGVCGSDAMNINGKNGLACITKVADLPEHVTLRPL"
BASE COUNT         1670 a         3651 c         3727 g         1914 t
ORIGIN      
        1 gcccgggcgg cgacacgatc atggccgcgt cgctctacgg gatcgcggac gccgacctgc
       61 aggcgctcgc gaagtacctc gccggcaagc cctaggccgc cttcgccgcc gacggcccgg
      121 ccttcgagat caactcgcgc agcacgtagg gcaggatgcc gccgtgcttg aagtagtcga
      181 cctcgatcgg cgtgtcgatg cgcgacttga ccctcacctc ctgcttcgcg ccgccggcgc
      241 gcgtgatcac gagcgtcagc tcctgctgcg gccggatgtc gtccaacccg agcacatcga
      301 tggtctcgtc gcccttcagg ccgagcgact gcgccgagtc ggaccccgtg aactgcagcg
      361 gcagcacgcc catgccgacc aggttggagc ggtggatgcg ctcgaagctg cgcacgatca
      421 ccgccttcac gccgagaagc tgcgtgccct tggccgccca gtcgcgcgat gagccggtgc
      481 cgtactcctc gccgccgaac accacggtgg gcgtgccttc cgcgatatag cgcatcgcgg
      541 cgtcgtagat gggcatctcc tggccctgga acttcgtcag gccgccttcc ttgcccccca
      601 tcatcaggtt cttgatgcgc acgttggcga acgtgccgcg catcatgacc tcgtggttgc
      661 cgcggcgcgc gccgtaggag ttgaagtcgg ccaccgcgac gtcgtgctcc tgcaggtaga
      721 tgccggcagg ggaggtcggc ttgatcgagc ccgcgggcga gatgtggtcg gtggtgaccg
      781 agtcgccgaa gatgccgagg accttcgcgc ccctgacctc gcccgggaga ttcggctgca
      841 tgtcgaactc ggcgaagaag ggcggctcgg cgatataggt cgaggtcggc cagctgtata
      901 cctgccccga aggcgccgag atcttcttcc acatcgggtt cgcgtcgccc aggttggcgt
      961 acaggcgccg gaaagtcggc gcatccatcg ccagcttgag cagggccttc acctcgtcgg
     1021 cgctcggcca gatgtggccg atgtacacgt cgcggccatc cttcgatttt ccgagcggct
     1081 cggtcttgaa gtccttcagc atggtgccgg cgatggcata cgccaccacc aggggcgggc
     1141 tggcgagaaa gttggcgcgg atgttggggt ggatgcgcgc ctcgaaattg cggttgcccg
     1201 agagcacggc cgcgcacacc aggtcgttct tgacgatcgc ctcttcgatc ggctgcgcca
     1261 gcggcccggc gttgccgatg cacgtggtgc acccgtaggc agccagattg aagccgagct
     1321 tttccaggta gggcatcagt cctgccttgg tgaggtactc ggtgaccacg cgcgagcccg
     1381 gcgcgagcga ggtcttcacg tgcggcttga ccgagaggcc gagctctacc gccttcttcg
     1441 ccaccagtcc ggccgcgagc agcacgcccg ggttggaggt gttggtgcag gaagtgatgg
     1501 cggcgatcag cacgtcgccg ttgcgcatct cggtgccatc gaaggcccgg tgcaccttgg
     1561 cgaggtcttc ctgcttccgg ccaaagccgt tctccgccgc cggcctcgaa tacagcgaag
     1621 tgaaggtcga tttgacgcgc tccaggttga tgcggtcctg cggccgcttc gggccggcca
     1681 gcgacggcat gacgctcgcg aggtcgagct ccagcgtctg gctgtagtcg atcgcaccaa
     1741 ccttgggcac gccaaacatg ccctgcgcct tgaagtacgc ggcgaaggcg tcgatctcgg
     1801 cgtcgctgcg cccggtgccg cggaagtact ccagggtctt ctcgtccacc gggaagaacc
     1861 ccatggtggc gccgtactcg ggcgccatgt tgccgatggt ggcgcggtcg gtgacgctga
     1921 gcgaggcggt gccctcgccg aagaactcga cgaacttgtt caccaccttg gcgttgcgca
     1981 gcatctcggt gacggtgagc accagatcgg ttgcggtgca ggccgggtgc aggcggccct
     2041 tcaggtgcac gccgaccacg tccggcgtca ggatgtacac cggctggccg agcatgccgg
     2101 cctcggcctc gatgccgccc acgccccagc cgaccacgcc gatcccgttg atcatggtgg
     2161 tgtggctgtc ggtgccgacg agcgtgtcgg gatagaacac gccgtccttc gacctgtgca
     2221 cgccgcgcgc caggtactcg aggttcacct gatggacgat gccgatgccc ggcggcacca
     2281 ccttgaacgt gtcgaaggcc tgcatgcccc acttcaggaa ctggtagcgc tcctcgttgc
     2341 gcctgaattc gagcttcatg ttcagatcga gcgcgttctt ggtgccgtag tgatcgatct
     2401 gaacgctgtg gtcgaccacc aggtccaccg gcaccagcgg ctcgatgacc ttggggttcc
     2461 tgcccatctt cttggccacg ccgcgcatgg cggcaaggtc gcagagcagc ggcacgccgg
     2521 tgaaatcctg cagcacgatg cgcgccagca cgaaagggat ctccgccgtg cgcgccgcgt
     2581 gcgcctgcca cccggcgagc tgcttcacgt gctcctcggt gaccttcttg ccgtcgcagt
     2641 tgcgcagcac cgattccagc acgatgcgga tcgagagcgg cagccggccg atattcacgc
     2701 ccagggcctg gccgagcttg ggcagcgagt agaacttgcc gcccttcagc ggctgcaagg
     2761 tgtcgtgcag gttatgcggc atgcgtgcct ccgtcattgg tcccgtgccc gaccgggtcg
     2821 caaggcccgt cgcgcgtgac tttgtcgagc tgcgccatgc ggcgcgcgcg ctgcgccagc
     2881 agctgggtca gctgctcgtc gcagcgcgcg atcggtgtcg gatagcggcg gatctccagc
     2941 gacagctcgg cgatgcggcc gcgcagctcg gcgaccgcgc tcatgtcctg cgcagctgca
     3001 cgtcgtaggt cagtccctgg tcgtcgcggc aggtgccgct gccggcggcg ccggtcacgc
     3061 ccttgaagtc gcagcgcagg cccttgccgt ctgcagagcg cagcagcgcc ttggcatccg
     3121 aaccgggtgc attgtcgacg atcaccgagg tgcccacgcc cgagcggcgg gtgccgaaca
     3181 ccccgcccac cacgaagccc gtgctgggcc cggggtcgct gaccacccag gtgccctcgt
     3241 aggtgcgctc tccgatggtg acgcgcagcc gcatctcccc gcccgtctgc ccggtcgcct
     3301 cgccgtagta gatctgtccg ctgtcgcgcg gcatcagggt gagcgagtag gtgctcgtgc
     3361 agccggcgag gagggcggcc gcggcggccg ccagcagccg cgtcatcacg ccaccatcat
     3421 gtccacgaag tcgcgcaccg gcgtggcgtc gagccgcggc gcatcgaggc acagctcgag
     3481 gatcgcctgc tgctgggggc gcggaaagcg ccgcgccaga ttggtcttga acttctccac
     3541 caggaccggc atgccttcct tgcgccggcg cttgtggccg atcgggtact cgcagaccac
     3601 ttccttcagc ttcgagccgt ccttgaactc cacggtaagc gcgttggcga tcgagcgctt
     3661 cttcgggtcg tggtagtccc gcgtgaagcg cttgtcttcg acgcagacga tcttcgcgcg
     3721 cagcgcgtcg atgcgcgggt ctcgcgccac gccgtcctcg tagtccgccg ccgtcaggcg
     3781 cccgaagatg agcggcaccg ccaccatgta ctggatgcag tggtcgcgat cggccggatt
     3841 gtggagcggc cctttcttgt cgatgatgcg gatcgccgcc tcgtgcgtgc ggatcttgat
     3901 gcgcttgatg tccttgatgc ggtccttgac cagcgcgtgc agctgcatgg cgcattcgac
     3961 cgcggtctgc gaatggaatt cggccgggaa cgagatcttg aacagcacgt tctccatcac
     4021 gtagctgccg aagccgcgct ggaacttgaa gggcttgccc ttgaacagga cgtcgtagaa
     4081 gccccaggtc ttcgccgtca gcaccgaggg atagcccatc tcgccggtct tggaaatgag
     4141 cgcaaggcgc acggcgcgcg aggtggcatc gcccgccgcc cagctcttgc gcgagccggt
     4201 gttgggggca tggcggtagg tgcgcagcga ctgcccgtcc agccaggcct gcgataccgc
     4261 gttcaccacc tggtcgtagg tgcagcccag catgtgcgcg accaccgcgg tggaggcgac
     4321 cttgaccagc acgacatggt ccaggccgac gcggttgaac gagttctcca gcgcgatcac
     4381 gccctggatc tcgtgcgcct tgatcatcgc ggtgagcacg tccttcatcg tgagcggctg
     4441 gccgtttctc gacagccagt cggccgtggc gaggatgccg ccgagattgt ccgacggatg
     4501 tccccactcg gcggcgagcc aggtgtcgtt gaagtcgagc cagcggatca tggcgccgag
     4561 gctgaaggcg gcctgcacgg gatcgagctg gaacttcgtg cccgggacct tggcgccgcg
     4621 cggggtcacg gtctcggggt cgattggccc gaggagcttg gtgcaggcgg ggtactcgag
     4681 cgcctcgaag ccgcagccca gggtgtccat caggcagtaa cgcgccgtgt cgtaggcctc
     4741 ggcgcgcttc acctggaatt tcgtcgcgta gtccgcgatc agcttgagca ccttgtccgg
     4801 cttgggccgg acgttggaga tgtgcgccga catcatttgt tctcggatag gggggcgaac
     4861 gtgtactcgt tgccgccggc cttgggtgcc ggacgcgccg gtgacggggc gccgcgcgtc
     4921 cgcggggtct tgtcccacca ggcagcgcgg ccgatcttct gcgactcgat ttcctcgggg
     4981 tgcgccgcca ggaattcgcg catgaaccgg gtcaggtcgg attcgtacgc cttcacttgc
     5041 ggtccttcag cggcacccat tgccggttct cggggccggt gtagttggcg ctcgggcgga
     5101 tgatcttgcc gtcgatccgc tgctcgatga cgtgcgccgc ccagccgctg gtgcgcgaaa
     5161 tcacgaacag cggcgtgaag tgcagcgtcg gcacgcccat cttgctgtag gacaccgcgc
     5221 tgaaccagtc gaggttggca aacatcttct tctcggccgc catcaccgac tcgatgcggt
     5281 cggcgatctc gaactgcgtc atgtccttcg cctcgcgcga caggcgctcg gccaccgcct
     5341 tgatcagctt gttgcgcggg tcggcgatcg tgtacaccgg gtgaccgaag ccgatcacca
     5401 cttccttggc ggcgacccgc ctgcggatgt cggattcggc ctcgtcggcg gacctgtagc
     5461 gcttctggat ctcgtcggaa acctcgttgg cgccgccgtg cttgggcccg cgcagggcgc
     5521 cgatgccgcc ggcgatggcg gagtacatgt ccgagcccgt gccggtgatc acacggcagg
     5581 tgaaggtgct ggcattgaac tcgtgctcgg cgtagagcac gagcgaggtg tgcatcgcgc
     5641 gcacgtgcga aggcgagggc ggcttgccgt gcaggaggtg caggtagtgc ccgccgatcg
     5701 agtcgtcgtc ggtcgcggtc tcgatgcgct tgccgccgtg cgcaaagtgg tgccagtaga
     5761 gcagcatcga gccgaagctc gccatcagcc ggtcggcgat ggcgcgcgcc ccctcgaccg
     5821 aatgcgccgg cgcttccggc tcgagcgtgc cgagcaccga gcagccggtg cgcagcacgt
     5881 ccatcgggtg cgcgctcttc ggcatttgct cgagcgcggt tctcagcggc gcgggcaggc
     5941 cgcgcagctt cttcagcttg gccttgtagg cagcgagctc ctgcgtcgtg ggcagcttct
     6001 cgtgcaccag caggtaggcg acctcctcga actccgccgc gtcggcgaaa tcgaggatgt
     6061 cgtagccgcg gtaatgcagg tcgttgccgg tgcggccaac cgtgcacagc gcggtgttgc
     6121 cggcggtgac gccggagagc gcgaccgact tcttgggctt aggagcgttg ggcgaagagt
     6181 tcatcgagtt tcttctcgta ggcgtgatag tcgagatagt cgtacagctc gtcgcgcgtt
     6241 tgcatcatgg cgacgacgtc cttctgcgtg ccctcgcggc gcagcgcctc gtacacggcg
     6301 agcgccgcct tgttcatggc gcggaacgcc gacagcggat agagcgcgat ggcaacgccg
     6361 gccgcgcgca gctcatcgag cgtgaacagc ggcgtcttgc cgaattcggt gatgttggcg
     6421 agcaccggga ccttcacggc gtcgacgaac ttgcggtact gcgcgagctc ggtgacggct
     6481 tccgggaaga tcatgtcggc gcccgcctcg gcgtacttca ccgcgcgcgc cagcgccgcc
     6541 tccagacctt cgccggccag cccgtcggtg cgcgccatga tgacgaacgc gcggtcggtg
     6601 cgggcgtccg cggcggcctt gatgcggtcg cacatctcct cggccgagac cagctccttg
     6661 ccggggcggt ggccacagcg cttggcgccg acctggtcct cgatgtgcgt cgccgcggcg
     6721 cccgccttga tcagcgatcg gaccgtgcgc ccgacgttga aggcgctcgc gccgaatccc
     6781 gtgtcgcagt cgaccagcag cggcaggtcg cacacgtcgg tgatgcggcg cacgtcgatc
     6841 agcacatcgt ccaggttgga gatgccgaga tcgggcatgc cgagcgagcc ggcggcgacg
     6901 ccgccgccgg agaggtagat cgccttgtag ccggtgcgct gcgcgaggcg cgcgtgatag
     6961 gcgcagatgg cgccaatcac ctggagcggc tgctcctcct tcagggcctg ccggaacctc
     7021 gcgcccgggc tcatgcgcca aggaggctct tcacgccgtc gcgctcttca tggagctcct
     7081 tcagcgtcgc ctccatgcgc ttgcgactga actccgacac ctcgatgcct ttgacgatct
     7141 catagcggcc gtccttgcag gtcaccgggt agccgtagag cacgccctcg gcgatgccgt
     7201 agctgccgtc cgagggcacg cccatgctca cccagtcccc cgcgggtgta ccgagcaccc
     7261 agctgcgcat gtggtcgatg gctgcattgg cggcgctggc ggcggaggaa aggccgcgcg
     7321 cctcgatgat cgccgcgccg cgcttttgca ccgtgggaat gaacgtgccc tcgagccagg
     7381 cctggtcatt gatcatcggc cagaccttct tgccctcgca ctccgcctgg aacaggtctg
     7441 ggtactgcgt cgccgagtgg ttgccccaca ccgtgacctt cttcaccgaa gcgtttggct
     7501 tgccgatctt gtgcgcgatc tgcgaggtgg cgcgattgtg gtccaggcgc atcatcgccg
     7561 tgaactgcgt gggcttgagg ctctttgcgt tcttcatcgc gatcaggcag ttggtattgg
     7621 cggggttgcc gaccaccagc acccgcacat cgcgcttcgc gaccttgtcg agggcctggc
     7681 cctgcggcgc gaatatcttg ccgttcgctt cgagcaggtc cttgcgctcc atcccggggc
     7741 cgcgcgggcg cgcacccacg agcagcgcga tgtcggcgtc cttgagtccc tgcatcgggt
     7801 cggaggccgc ctgcatgccg gcgagcagcg gaaacgcgca gtcatcgagc tccatcatca
     7861 cgcccttcaa cgccttttgc gccttctcgt ccgggatttc gagcagctgc aggatcactg
     7921 gctggtcggt tccgagcatg tcgccgctgg cgatgcggaa caagagcgag tagccgatct
     7981 ggccggcggc accggtgacg gcgacgcgga cgggcgcttt gagcatgggc ggaagctccg
     8041 aagacgtggc gaactggttc cggaattcta ccgcagcgcg cgcgccaaac gcagtgcaca
     8101 aaaggaactc aatcaatgcg ctacggcgct ataatcgtta tcggtggcgc ggagcgccta
     8161 cccttcacat ggcgaacgta acgataagaa agccgaggcc gaagtacctc agcctgacgg
     8221 cactcctgtt cgagatccgg ctacccctgc cgggctgggt atcgatcctg catcggatca
     8281 gcggcttgct gcttttcctg gccgtggtct ggctgctgtt catgctcgac cgcagccttg
     8341 cgtccgaggc ggacttcgag gcgatacgcc gctatgtcgg cctgctgccg gtcaagctgt
     8401 ctctgctcgt cctgatctgg gccttttgcc accacttctg cgccggcatc cgtttcctgc
     8461 tcctcgacct cgaccggggc gtcgacaagg ccactgcgcg attcacgagc tggatcaccc
     8521 tcgccgcaag ccttgcgctc accgcgtggc tgggagcgaa gctgtggtag atcgcgtcgt
     8581 cactggcgcg cactacggcc tgcgcgactg gctcgcgcaa cgcgtcacgg ccgcggtcat
     8641 ggcggtgtac acggtgatca tgctcgcggt gctgatcggc gccgcgccga tcggctacgc
     8701 cgtatggaag gacctgttta cgcagggctg gatgcgcgtg gcgacgttgc tgttcgccgc
     8761 gagcctcgcc tggcacgcct gggtcggcat gcgcgacatc ctgatggact acgtgaagcc
     8821 cacgggcgcg cggcttacgc tgcaggtcgg cgtgctgctc gtcatcgcgg cgtatctcgc
     8881 gtggaccatc cagatcctgt ggcgctgagg caatgagcga tctcccggtt cgccggttcg
     8941 atgccgtcgt cgtgggcgcg ggcggctcgg gcatgcgcgc ggcgctcgag ctcgcgcgcg
     9001 ccgacctcaa ggtggcggtt ctcaccaagg tattcccgac gcgctcgcat accgtggcgg
     9061 cgcagggcgg catcgcggcg ccgctcgcca attccaccga agacaactgg cattggcaca
     9121 tgtacgacac cgtcaagggg tcggactacc tcggcgatca ggatgccatc gagtacatgt
     9181 gccggcgcgc gaccgaggtc gtggtcgagc tcgagcacat gggcatgccg ttcgaccggc
     9241 tggagaacgg caagatctac cagcgcccgt tcggcggcca cacgcaggac tacggcagcg
     9301 cgaagatggc catgcgcgcc tgcgcggcgg ccgaccgcac cgggcacgcc atgctgcaca
     9361 cgctgtatca gcagaacgtg cgcgccaata cgcaattctt cgtcgaatgg atggcgctcg
     9421 acctggtgcg cgacccgcag gacggcgcgg tgatcggcgt cacggcgctg gaaatggaaa
     9481 ccggcgaagt cacgctgttc caggcgaagg cgatcctgct cgcgaccggc ggcgcagggc
     9541 gcatcttcgc cgcgagcacc aatgccttca tcaacaccgg cgacggcctc ggcatggcgg
     9601 cgcgcgccgg actgccgctg caggacatgg aattctggca gttccacccg acgggcgtct
     9661 atggcgcggg cgtgctgatc accgagggcg tgcgcggcga aggcggctac cttctgaaca
     9721 aggacggcga gcgcttcatg gagcgctacg caccgaacgc caaggatctg gccagccgcg
     9781 acgtggtgtc gcgcgccatg accaccgaaa tcaaggaagg gcgcggctgc ggccccgagg
     9841 ccgaccacgt gctgctcaag ctcgaccacc tggggccgga ggtgatcaac cagcgcttgc
     9901 cgggaatccg cgagatcgcc atcaagttcg ccaatgccga tccggtccgc gagccggtac
     9961 cggtggtgcc gacggtgcac taccagatgg gcggcgtccc ggccaactac atggggcagg
    10021 tggtggcgcc cgagggcgcg agccaggaag ccatcgtgcc gggcctgtac gccgcggggg
    10081 aatgctcatg cgtctccgtg cacggggcca accgcctcgg caccaattcg ctcctcgacc
    10141 tgctggtctt cggcaagtcc tcgggcgagc agatggccaa ggacatccgc gcgctgccgc
    10201 gcccgcaccg caacctgccg cgggactcgg gcgaggcttc gcgcgcgcgc ctggcgcggc
    10261 tcgatgcggc ccgcgggggc gagcgagtcg ccgaggtcgc cgcggagctg cggcgcgtga
    10321 tgcaggcgca ttgcggcgtg ttccgttttc cggaggatct ggcggccggc gtggagaaga
    10381 tgacgcagat cgcccggcgc gccgcgaacg ttgccatcca cgaccggtcc aaggtgttca
    10441 ataccgcgcg catcgaggcg ctggagctgg acaacctcgt gcagaccgcg atgtcgacgg
    10501 tggtgtccgc gcaggcgcgc aaggaaagcc gcggcgcgca gtcgcgcgcc gacttcccgg
    10561 agcgcgacga cgcgcactgg atgaagcaca cgctctggta caaggaaggc aaccgcctgg
    10621 cgtacaagcc ggtgcacctc aaggcgctga cggtcgaatc cttcgagcca aaggttcgaa
    10681 cctactagga cccgccgatg aagctgtcga tctaccgcta cgacccggac aaggacgcca
    10741 agccctacat gcaggagctc gaggtgacgc tcgagcattc ggaccgcatg ctgctcgacg
    10801 ccctcgtcaa ggctaaagcg caggacgaca gcctcgcctt ccggcgctcc tgccgcgagg
    10861 gcgtgtgcgg gtccgatgcc atgaacatca acggcaagaa cggcctcgcc tgcatcacca
    10921 aggtggccga cctgccggag cacgtcacgc tgcggccgtt gc
//
LOCUS       DBNHKE010000232         7187 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1208727, whole genome shotgun sequence.
ACCESSION   DBNHKE010000232 DBNHKE010000000
VERSION     DBNHKE010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1208727"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC16O9_15865"
     CDS             complement(<1..203)
                     /locus_tag="AC16O9_15865"
                     /inference="COORDINATES: protein motif:HMM:NF017559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR6518133.1"
                     /translation="MTAGPRPWYREPWTWIVVAPPATAVVAGLATIWIATSGADGLVA
                     EDYYKQGLALNKVIAREERARALA"
     gene            complement(200..835)
                     /locus_tag="AC16O9_15870"
     CDS             complement(200..835)
                     /locus_tag="AC16O9_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR6518134.1"
                     /translation="MSGGQPIKVWDLGVRLFHWTLVLAYAVAYLSGEVESDRLHIIAG
                     YAVLALIAFRLVWGFVGTRHARFVDFVYGPAATWRYARSMLRRAPLHYLGHNPLGGWM
                     VVALLLSLAAACWSGLEAYGAQGHGPLAQAGLVAPAYAHGDGGRDADDDEAREAEGDE
                     FWEELHELFSHLTLLLVALHIAGAVAASLLHGENLVKAMITGYKRPHEPQT"
     gene            complement(854..2233)
                     /gene="ccoG"
                     /locus_tag="AC16O9_15875"
     CDS             complement(854..2233)
                     /gene="ccoG"
                     /locus_tag="AC16O9_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HIR6518135.1"
                     /translation="MKARGAPAQVVEQALYEVRKAIHPRAVHGWFAGWRWALVWATQI
                     VFYGLAWLPWNGRQAVLFDIAHRKFTIFGLVFFPQDIIYLTVLLVIAALSLFLFTAVA
                     GRLWCGYACPQTVYTEIFLWVEEKIEGDRPARLKLDAAPLSARKLALKVAKHGAWIAI
                     ALWTGFTFVGYFTPIRELWHAVWSFSTGPWETFWVLFYGFATYGNAGWMREQVCKYMC
                     PYARFQSAMFDPDTLIITYDRERGEPRGKGKDKGHCVDCSICVQVCPTGIDIRDGLQY
                     ECIGCAACIDGCDRVMEKVGFPKGLIRYSTTYAMEQKLTRAEMFRRAFRPRVLLYSAI
                     LWAIIIAAGVALWNRVPLKVDVIRDRAAISRETAGGQIENVYRLQIMNTHESARRFDI
                     GVAGLPGATLAGEAAVVLDGAQARMVPVRVRVPAGALAPGTHPLAFTVRARDDAGVAV
                     RERSVFIVR"
     gene            complement(2230..3129)
                     /gene="ccoP"
                     /locus_tag="AC16O9_15880"
     CDS             complement(2230..3129)
                     /gene="ccoP"
                     /locus_tag="AC16O9_15880"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR6518136.1"
                     /translation="MNEFTSSFWDLYIAILTVVSILACGVLLLAQDKRRVAGGTSETT
                     GHTWDEDLGEYNNPLPRWWLWLFWITIVFSLVYLALYPGLGSYQGLWQWTQVSQLEAE
                     TARHEEKFGPLYERYAQMDVKALAAEPRALAIGQKLFLNNCAQCHASDGAGSRGFPDL
                     TDRDWQWGGTPEAIKTSITQGRTGVMPPMGAALGEQGTKDAAHYVMSLSGLPHDTMRA
                     ARGKPLFAQNCAACHGAEGAGNPMLGAPNLADGTWLYSAAEPVIVETISQGRTNQMPA
                     HQELLGKARIHLLTAYVLSLSQK"
     gene            complement(3126..3269)
                     /locus_tag="AC16O9_15885"
     CDS             complement(3126..3269)
                     /locus_tag="AC16O9_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase subunit 3"
                     /protein_id="HIR6518137.1"
                     /translation="MDLNLLRSLVTVAALVAFLGIVWWAYSPARKARLEDAGRSVLEE
                     PER"
     gene            complement(3278..3895)
                     /gene="ccoO"
                     /locus_tag="AC16O9_15890"
     CDS             complement(3278..3895)
                     /gene="ccoO"
                     /locus_tag="AC16O9_15890"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR6518138.1"
                     /translation="MKLTHDMIERNQALLIVLVILVVAVGGLVEIVPLYFQKYVTEPV
                     AGLKPYSALQLTGRDVYIREGCYNCHSQMVRPFRAEVERYGRYSVAGEFVYDRPFQWG
                     SKRTGPDLHRVGGRYTDEWHRLHLLNPRDLVPESNMPAYPWLARDAANAADIGAKMRA
                     LRTLGTPYSDEEIARAPEALKGKTEMDALIAYLQVLGTAGRAGAK"
     gene            complement(3906..5336)
                     /gene="ccoN"
                     /locus_tag="AC16O9_15895"
     CDS             complement(3906..5336)
                     /gene="ccoN"
                     /locus_tag="AC16O9_15895"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR6518139.1"
                     /translation="MSSSETTYNYRVVRQFSIATIVWGVVGMLVGVIIAAQLLWPELN
                     LGIPWFSYGRLRPLHTNAVIFAFGGSALFATSYYVVQRTCHARLFSDRLAAFTFWGWQ
                     AVIVLAAITLPLGITTSKEYAELEWPIDILIALVWVAYAVVFFGTIWKRRVAHIYVAN
                     WFFGAFILTVALLHIVNSAAMPATFWKSYSAYAGAQDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFIPKQAGRPIYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWAQSLGMVFSL
                     ILLAPSWGGMINGIMTLSGAWHKLRTDPILKVLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTIGHVHSGALGWVGLISMGAMYYLIPRLFGRTEMWSVRLINVHFWVATIGIV
                     LYIAAMWIAGVMQGLMWRAVGEDGILTYTFVESVKATYPFYAIRLLGGVAYLAGMVLM
                     AYNVAKTVGAGKSAEAPIPAPAMAHA"
     gene            5575..6117
                     /locus_tag="AC16O9_15900"
     CDS             5575..6117
                     /locus_tag="AC16O9_15900"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR6518140.1"
                     /translation="MAKKQPFLTDIKTLRARARRHIEQGAVTESYRADRKTVIKLLNE
                     ALATEIICVLRYRRHHFMAAGINADSVAAEFLVHANEEQQHADQIAARIVQLQGEPNF
                     SPEGLASRAHAEYVEGENLIDMIKENLVAERIAIDSYREMIEYVGDKDTTTKRMLEGI
                     LAMEEEHADDLVGMLEELDT"
     gene            complement(6114..7088)
                     /locus_tag="AC16O9_15905"
     CDS             complement(6114..7088)
                     /locus_tag="AC16O9_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6518141.1"
                     /translation="MRAMRLHAPGKPLALDDVPVPEAAPGQVLVKVLACGVCRTDLHV
                     ADGDLSECSYPVVPGHEIVGRVEKGGTRFARGARVGIPWLGWTCGACEYCKCGRENLC
                     PKARFTGYTLQGGFAEFAVADERFCFALPERYGDAEAAPLLCAGLIGYRSYAMAGEGK
                     RLGLYGFGAAAHVLAQVARAEGREVYAFTRPGDAKAQAFAKRLGAVWAGDSDAAPPAP
                     LDAAILFAPVGALVPQALRAVKQGGCVVCGGIHMSDIPSFPYALLWGERRVQSVANLT
                     RADGADFLDLAARAPIRTEVKTYSLEHANEALGDLRAGRLEGAAVLVP"
BASE COUNT         1241 a         2495 c         2386 g         1065 t
ORIGIN      
        1 gcgagcgcgc gcgcgcgctc ctcccgcgcg atgaccttgt tgagggcgag gccctgcttg
       61 taatagtcct cggcgaccag accgtcggcg cccgaggtgg cgatccagat cgtcgccagg
      121 ccggcgacca ccgcggtggc gggtggcgcc accacgatcc aggtccaggg ctcgcgatac
      181 caggggcggg gtccggcagt catgtctgag gttcgtgagg tcgcttatac ccggtgatca
      241 tcgccttcac caggttctcg ccatgcagga ggctcgcggc gacggcgccc gcgatgtgca
      301 gcgccacgag cagcagggtg aggtgggaaa agagctcgtg cagctcctcc cagaattcgt
      361 cgccctccgc ctcgcgggcc tcatcgtcgt cagcgtcccg cccgccgtcg ccgtgcgcgt
      421 acgccggggc aaccagcccg gcctgggcca gcgggccatg gccctgtgcg ccgtacgctt
      481 ccagcccgct ccagcacgcg gccgcgagcg acagcagcag cgccaccacc atccagccgc
      541 ccaggggatt gtggccaagg taatgcaatg gcgcgcgcct caacatcgac ctggcgtacc
      601 gccaggtcgc cgccggaccg tagacgaagt cgacgaagcg cgcgtgccgc gtgccgacaa
      661 agccccacac cagccgaaac gcgatgagcg cgaggactgc gtagccggcg atgatgtgca
      721 gccggtcgct ctccacttcc ccgctcagat aggccacggc gtacgcgagg accagcgtcc
      781 agtgaaacag ccgcacgccg agatcccaga ccttgatcgg ctgcccgccg ctcatgtgag
      841 tcgctccatc cgactaacgc acgatgaaca cggatctctc gcgcaccgcg acgcccgcat
      901 cgtcgcgcgc ccgcaccgtg aacgccaacg ggtgcgttcc cggggccagc gcgcccgcag
      961 gaacgcgcac gcggaccggc accatgcgcg cctgtgcccc gtccaggacc accgcggcct
     1021 cgccggccag cgtcgccccg ggcaggcctg cgacgccgat gtcgaagcgc cgcgcggatt
     1081 cgtgcgtgtt catgatctgc agtcggtaca cgttctcgat ctgcccgccg gcggtctcgc
     1141 gcgagatcgc ggcgcgatcg cggatcacgt ccaccttgag cggcacccga ttccacagtg
     1201 cgacccctgc ggcgatgatg atcgcccaca ggatcgcgct gtagagcagc acacggggac
     1261 ggaaggcgcg cctgaacatc tcggcgcgcg tcagcttctg ctccatcgcg tacgtggtcg
     1321 agtagcggat cagccctttg ggaaagccca ccttctccat gacgcggtcg cagccgtcga
     1381 tgcacgccgc gcagccgatg cactcgtact gcaggccgtc gcggatgtcg atgcccgtgg
     1441 ggcagacctg cacgcagatg ctgcagtcca cgcagtggcc cttgtccttg cccttgccgc
     1501 gcggctcgcc gcgctcgcgg tcgtaggtga tgatcagcgt gtccgggtcg aacatcgcgc
     1561 tctggaagcg cgcgtacggg cacatgtact tgcacacctg ctcgcgcatc cagccggcgt
     1621 tgccgtaggt ggcgaagccg tagaacagca cccagaacgt ctcccacggc ccggtggaaa
     1681 acgaccacac cgcgtgccac agctcgcgga tcggcgtgaa gtagccgacg aaggtgaacc
     1741 cggtccacag cgcgatcgcg atccatgcgc cgtgcttggc gaccttcagc gcgagcttgc
     1801 gcgcgctgag cggcgccgcg tcgagcttca gccgtgccgg ccggtcgcct tcgatcttct
     1861 cctccaccca gaggaagatc tccgtgtaca cggtctgcgg gcaggcatag ccgcaccaca
     1921 ggcggccggc gaccgcggtg aacagaaaca acgacagcgc cgcgatgacc aggagcaccg
     1981 tgagatagat gatgtcctgc gggaagaaca ccagcccgaa gatggtgaac ttgcggtgcg
     2041 cgatgtcgaa cagcaccgcc tggcggccgt tccagggcag ccacgccagg ccgtagaaca
     2101 cgatctgcgt cgcccatacc agcgcccagc gccagcccgc aaaccacccg tgcaccgcgc
     2161 gcgggtggat cgccttgcga acctcgtaca gcgcctgctc gacgacctgt gctggtgcgc
     2221 ctcgcgcttt cacttctgcg acagcgacag cacgtacgcg gtgagcagat gaatcctcgc
     2281 cttgccgaga agttcctggt gcgcgggcat ctggttcgtg cgtccctggg agatggtctc
     2341 gacgatcacc ggctcggccg cgctgtacag ccatgtgccg tcggcaagat tgggcgcgcc
     2401 gagcatcggg ttgcccgcgc cctcggcgcc gtgacaggcg gcgcagttct gcgcgaacag
     2461 cggcttgccg cgcgcggcgc gcatggtgtc gtgcggcagg cccgagagcg acatgacgta
     2521 gtgcgccgcg tccttcgtgc cctgctcgcc cagcgccgcg cccatcggcg gcatgacgcc
     2581 ggtgcgtccc tgggtgatgc tcgtcttgat cgcctcgggc gtgccgcccc attgccagtc
     2641 gcggtcggtg aggtcgggaa agccgcgcga gccggcgccg tcggaggcgt ggcactgcgc
     2701 acaattgttg aggaacagct tctgcccgat ggcgagcgcg cgcggctcgg cggcgagcgc
     2761 cttcacatcc atctgcgcgt agcgctcgta gagcggcccg aacttctcct cgtgccgcgc
     2821 cgtttccgcc tcgagctgcg acacctgggt ccactgccac aggccctggt agctcccgag
     2881 cccggggtag agcgccagat agaccaggct gaacacgatc gtgatccaga acagccacag
     2941 ccaccagcgc ggcagcgggt tgttgtactc gcccaggtcc tcgtcccagg tatgaccggt
     3001 ggtctcgctc gtgccgccgg cgacgcggcg cttgtcctgc gccagcagca gcacgccgca
     3061 ggcgaggatc gagaccaccg tcaggatcgc gatgtagaga tcccagaagc tgctcgtgaa
     3121 ctcgttcatc gctcgggctc ctcgaggacg ctgcgcccgg cgtcttccag gcgggccttg
     3181 cgtgcgggcg agtaggccca ccagacgatg ccgaggaagg ccacgagggc cgccacggtg
     3241 accaggctgc ggagcaggtt gaggtccatg cgccccccta tttcgcgccg gcgcgccccg
     3301 cggtgccgag cacctgcagg taggcgatga gcgcatccat ctcggtcttg cccttgagcg
     3361 cctcgggcgc gcgggcgatc tcttcgtcgg aatacggcgt gccgagcgtg cgcagcgcgc
     3421 gcatcttggc gccgatgtcg gccgcgttgg ccgcgtcgcg cgcgagccac ggataggccg
     3481 gcatgttgga ctcgggcacc aggtcgcgcg ggttgagcag gtgcaggcgg tgccattcgt
     3541 ccgtgtagcg cccgccgaca cggtgcaggt cggggccggt gcgcttcgag ccccactgga
     3601 acgggcggtc gtagacgaac tcgcccgcca ccgagtagcg cccgtagcgc tcgacctcgg
     3661 cgcggaacgg ccgcaccatc tgcgagtggc agttgtagca gccctcgcgg atgtagacgt
     3721 cgcggcccgt gagctgcagc gcgctgtagg gcttcagtcc cgcgaccggc tcggtgacgt
     3781 acttctggaa gtacagcggc acgatctcca ccaggccgcc gaccgccacc accaggatca
     3841 ccaggacgat gagcagcgcc tggttgcgtt cgatcatgtc gtgggtcagt ttcatcgaac
     3901 gctccctagg cgtgcgccat cgcgggcgcc gggatcggcg cctccgcgct cttgccggcg
     3961 ccgacggtct tcgccacgtt gtaggccatg agcaccatgc cggcgaggta cgccacgccg
     4021 ccgagcaggc ggatggcgta gaacggatag gtcgccttca cgctctcgac gaaggtgtag
     4081 gtgagtatgc cgtcctcgcc caccgcgcgc cacatcaggc cctgcatcac gccggcgatc
     4141 cacatcgcgg cgatgtagag cacgatgccg atcgtggcga cccagaagtg cacgttgatc
     4201 aggcgcacgc tccacatctc ggtgcggccg aacaggcgcg gaatcaggta atacatcgcg
     4261 cccatcgaga tcagcccgac ccagccgagc gcgccgctgt gcacgtggcc gatggtccag
     4321 tcggtgtagt gcgagagcgc gttcacggtc ttgatcgaca tcatcggccc ctcgaacgtg
     4381 ctcatgccgt agaacgacag cgacacgatg aggaccttca ggatcgggtc ggtgcgcagc
     4441 ttgtgccacg ccccggagag cgtcatgatg ccgttgatca tcccgcccca ggagggcgcg
     4501 agcaggatca gcgagaacac catgccgagc gactgcgccc aatcgggcag cgcggtgtag
     4561 tgcaggtggt gcgggccggc ccacatgtag gtgaagatca gcgcccagaa gtgcaccacc
     4621 gacaggcggt aggagtagat cgggcgcccc gcctgcttgg ggatgaagta gtacatcatc
     4681 ccgaggaacc cggcggtgag gaaaaagccc accgcgttgt gcccgtacca ccactgcacc
     4741 atcgcatcct gcgcgccggc gtaggccgag taggacttcc agaacgtggc cggcatcgcg
     4801 gcgctgttca cgatgtgcag cagcgccacc gtcaggatga aggcgccgaa gaaccagttg
     4861 gcgacgtaga tgtgcgcgac cctgcgcttc cagatcgtgc cgaaaaagac gaccgcgtag
     4921 gccacccaca ccagggcgat caggatgtcg atcggccact cgagctcggc gtactccttg
     4981 gaggtggtga tgccgagcgg cagcgtgatc gccgcgagca cgatgaccgc ctgccagccc
     5041 cagaaggtga acgcggcgag gcggtccgag aacaggcgcg cgtggcaggt gcgctgcacg
     5101 acgtagtagg aggtcgcaaa gagcgccgag ccgccgaagg cgaagatcac cgcgttggtg
     5161 tgcagcggcc tgaggcggcc gtaggaaaac cagggaatgc cgaggttcaa ttcgggccag
     5221 agcagctgcg cggcgatgat tactccgacg agcatcccga ccacgcccca gacgatggtg
     5281 gcgatcgaga actgccgtac aacccggtag ttgtaggtgg tttcgctgct cgacattccc
     5341 gcccccctgg tggcgtaccg aaggcgatgc tagcgagcga ggccgccccg gagttgatct
     5401 gcgtcaagca gggtccccga tagccggcgg aaatgcccgg cattggatta atcaacttca
     5461 cgcatggggc attgggcggt tcaatacgcc cctgccacgc gctgcgccgt acggtctcgg
     5521 gtgcaaccgc gctgatgatg gtcctggaca acgtgttcgc ataggagaaa gaagatggcc
     5581 aagaagcagc cgttcctcac cgatatcaag accctccgcg cccgcgcgcg caggcacatc
     5641 gagcaggggg cggtgaccga gagttaccgc gccgaccgta agaccgtcat caagctcctc
     5701 aacgaggcgc tcgccaccga gatcatctgc gtgctccggt atcgccgcca ccacttcatg
     5761 gccgcgggca tcaacgccga ttcggtcgcg gccgagtttc tcgtgcacgc caacgaggag
     5821 cagcagcatg ccgaccagat cgcggcgcgc atcgtgcagc tgcagggcga gccgaacttc
     5881 tcccccgagg gcctcgcctc gcgcgcgcat gccgagtacg tcgagggcga gaacctgatc
     5941 gacatgatca aggaaaacct ggtcgccgag cggatcgcga tcgacagcta ccgcgagatg
     6001 atcgagtacg tcggcgacaa ggacaccacc accaagcgca tgctcgaagg catcctggcg
     6061 atggaagaag agcacgccga cgacctcgtc ggcatgctcg aggagctcga cacctagggc
     6121 accagcaccg ccgcgccttc gagacggccc gcccggagat ccccgagcgc ctcgttcgcg
     6181 tgctccaggg aataggtctt gacctcggtg cggatcggcg cgcgcgccgc gaggtcgaga
     6241 aagtccgcgc cgtcggcgcg cgtgaggttc gcgaccgact gcacgcgccg ctcgccccag
     6301 agcagcgcgt acggaaacga cgggatgtcg ctcatgtgga tgccgccgca gaccacgcag
     6361 ccgccctgct tcaccgcccg cagcgcctgc ggcacgagcg cccccaccgg cgcaaacagg
     6421 atcgccgcgt cgagcggcgc gggcggcgcc gcatccgaat cccccgccca caccgcgccc
     6481 agccgcttcg cgaacgcctg cgcctttgcg tcgccgggtc gcgtgaacgc gtagacctcg
     6541 cgcccctcgg cgcgtgccac ctgcgccagt acatgcgccg cggcgccgaa tccgtacaac
     6601 cccagccgct tgccctcgcc cgccattgcg tacgagcggt agccgatgag gcccgcgcag
     6661 agcagcggcg cggcttcggc gtcgccgtag cgctcgggaa gcgcgaagca aaagcgctcg
     6721 tcggccaccg cgaactccgc gaagccgccc tgcagcgtgt agcccgtgaa gcgcgctttg
     6781 gggcacaggt tctcgcgccc gcacttgcag tactcgcagg cgccgcaggt ccagccgagc
     6841 caggggatgc ctacgcgcgc gccgcgcgcg aagcgtgtac cgcccttctc cacgcggccg
     6901 acgatctcgt gtccggggac cacgggatag ctgcattcgg acagatcgcc gtcggcgacg
     6961 tgcagatcgg tgcggcacac gccgcaggcg agcaccttga cgagcacctg ccccggcgcc
     7021 gcctccggca ccggcacgtc atcgagcgcc agcggcttcc ccggcgcatg taaacgcatg
     7081 gcacgcataa atggggacag acctcattat ttcgcagagc gaaaaaatga ggtctgtccc
     7141 tatttatcgt cgtccatcag aacgtcgtgc gccgggcgct cgaggtc
//
LOCUS       DBNHKE010000233        31506 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_256986, whole genome shotgun sequence.
ACCESSION   DBNHKE010000233 DBNHKE010000000
VERSION     DBNHKE010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 31506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_256986"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="AC16O9_15910"
     CDS             <1..606
                     /locus_tag="AC16O9_15910"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR6518142.1"
                     /translation="YSTKIDMQEFKKYRGLFAEQGDFDIPENYNLGVAFKANPQLTIA
                     ADYKRINYSKVASVGNSSRQGGCAPTMPAGPGVGSGCLGASGASIGFGWQDVNVFKIG
                     VEYQMNSKLLLRAGYNLTDNPINASDVTFNILAPGVVEDHLTLGFTYALQGGSEITMA
                     YMHAFKNSVTGPASNPYFGVGGTETISLAEDSLGISWGMKF"
     gene            588..1271
                     /locus_tag="AC16O9_15915"
     CDS             588..1271
                     /locus_tag="AC16O9_15915"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HIR6518143.1"
                     /translation="MGNEVLGGAAKAVLLDLDGTLLDTAPDLAAAVNAMLAGQGLKPL
                     PAAAIRDYIGKGIPHLVERCLVEAGLPLTCAQLEPALRAFGDHYRAFNGKSSRPYPGV
                     VEGLQRLRAASLKLACVTNKARTFTTPLLEATGLAPLLDAVVTSDEVGRRKPHPEPYL
                     HACKLLQVAPEEAVVIGDSANDAEAGRSAGCRVLLVSYGYSEGRDVRELGADAIGAEF
                     RDAVDLLLG"
     gene            1366..1794
                     /locus_tag="AC16O9_15920"
     CDS             1366..1794
                     /locus_tag="AC16O9_15920"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6518144.1"
                     /translation="MKATRILAALLLAAAALSPFAVHADKGGRHQIVIQVTDNDPAKW
                     NLALNVAQNLQQAYGKDKTDVEIVAYGPGLNMFKSGSTVAKRLNAAQDASVALYACEN
                     TMQKMKVTTADLHPGSVVAPGGGVVHVFKRQREGWDTIRP"
     gene            complement(1802..2224)
                     /locus_tag="AC16O9_15925"
     CDS             complement(1802..2224)
                     /locus_tag="AC16O9_15925"
                     /inference="COORDINATES: protein motif:HMM:NF015581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR6518145.1"
                     /translation="MADPHPVVSYSKRANFEDVRDDLKLAIESRGLVIDYHSFVGRML
                     ERTGKDVGSAKKLYADAQAFVFCSAALSRKTMEADPANAAFCPYRIIVYATAQDPGTV
                     HVAYQRPWRPDGSAASQAALKEVDALLDGLAKEAVGLG"
     gene            2278..3165
                     /locus_tag="AC16O9_15930"
     CDS             2278..3165
                     /locus_tag="AC16O9_15930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6518146.1"
                     /translation="MIRIKRCAAALCLALIAAPAAATHEPWVTERFPQDEAKWWWDAE
                     WWERGRIPQTPGHEVASRPASYRSGDVEVPVEIHAPKGAGRFPVVVFLHGRRGIDPLT
                     RLVPLRLAARGFTVVAPDLYAGRFIAPFPIRHDEALEEDAARAIDFALALPEARGQAK
                     TCVVSHTRGGYYSLKALVTHGRQAKTACYASYYPHWQHPEAPEPEQVYRYAREVEALE
                     VPVLVFFGEHEQYQRARPILAGIEALQKKGRDARVLVYPGVGRGFDFRPREVRTFADD
                     LAAQDAMRRTAEFMRRHLP"
     gene            3162..3638
                     /locus_tag="AC16O9_15935"
     CDS             3162..3638
                     /locus_tag="AC16O9_15935"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6518147.1"
                     /translation="MNPARTFRWLALAALLASTQAFAQVAKVVWHVDFADARRLSAMI
                     QNVNNMVTTYQGNLEDYEVHVVFLAGGIRFVTTDPLKGTPFAEDEAFRKARPDLITRL
                     QQLRTLHQVKLELCEITREQLNLPKDKIIEGVAPVRSGVVRIAELQSKGYAYLKVD"
     gene            complement(3642..4538)
                     /locus_tag="AC16O9_15940"
     CDS             complement(3642..4538)
                     /locus_tag="AC16O9_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HIR6518148.1"
                     /translation="MRPAWSVLLLTTLIGAGQGLYLALYAAELAGLGGSTLFVAGAAL
                     SLALLGAGLLASFFHLGRPERAWRSAARWRSSWLSREVLVLPAFMAAVAAWAAAHWLA
                     QPTLALGAAGAVLALALFICTGMIYACLPFLQEWRTPLTIVNFTLLGSASGLTLAAAL
                     AAWLHAPLARPYVIAALVVGAAAYLGRLAALARNTRLRPKSTLATAIGVKHPRIVQIA
                     QGAMGGSFNTREFFHGRPQEVVRAVRWALLALVFPVPALLLLYGQPGAAFLLQFAGLL
                     AERWYFFAEAKHPQNLYYQAIA"
     gene            complement(4542..5279)
                     /locus_tag="AC16O9_15945"
     CDS             complement(4542..5279)
                     /locus_tag="AC16O9_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6518149.1"
                     /translation="MTTKQLALVIDLNVCVGCHACVTSCKEWNTAGAAGPLSDQNPYG
                     ADPTGTFFNRVQTFEVGEYPKTETVHFPKSCLHCEDPPCVPVCPTGASYKRAEDGIVL
                     VDYDKCIGCKYCSWACPYGAREVDGQQKVMKKCTLCVDRIYDAQLPEAERKPACVLAC
                     PTSARLFGDIHDPESQASRAIREQGGYALMPEWGTHPANHYLPRRKTEKRLHPDELER
                     HDNPRKIDGLLPQPSPQAPALDDVTSW"
     gene            complement(5279..7972)
                     /locus_tag="AC16O9_15950"
                     /pseudo
     CDS             complement(5279..7972)
                     /locus_tag="AC16O9_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853975.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
     gene            8077..8601
                     /locus_tag="AC16O9_15955"
     CDS             8077..8601
                     /locus_tag="AC16O9_15955"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6518150.1"
                     /translation="MKICIVSDSHDRADALARAVREAKAAGAEAVIHCGDLIGAQTVK
                     PALAAGLPVHLIHGNNIGDTQALHRQSRMSGGQLQYHGADARLELGSRRVFVVHYDDY
                     GYAMACTGEWDLVCCGHSHKAEAREVTGVNGGRTWLVNPGTVAGLSAPATWLLGDLAS
                     MRFEIRALNASISA"
     gene            8670..9482
                     /locus_tag="AC16O9_15960"
     CDS             8670..9482
                     /locus_tag="AC16O9_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518151.1"
                     /translation="MFTSNPFATLSASVPAEFMQGFVLLMLVLVAAGTLFEVVHKGSA
                     KYFFQNWRNSRSKAAKPLGGGELVAIAVKTALVDVAASGEFCNMRRRVAHLLTMYGFV
                     LYVIATAVMVFDLSARGLVAQLWWLGGLMVLAGGYWFWFFIRVDVAAEGNSPLRVVYA
                     DVFILSLLVSVTLGLVWAGAGGGAGVLFALYVLTTAVLFGSVPWSKFAHMFFKPAAAF
                     EKRVSNANGSAENLPTLTRDDPEQQKRHSMDLLRDAPMDMGLGIKREAPRHY"
     gene            9508..9987
                     /gene="aprB"
                     /locus_tag="AC16O9_15965"
     CDS             9508..9987
                     /gene="aprB"
                     /locus_tag="AC16O9_15965"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019418 - sulfide oxidation [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HIR6518152.1"
                     /translation="MPTFVYMTRCDGCGHCVDICPSDIMHIDKTYRRAYNIEPNMCWE
                     CYSCVKACPQHAIDVRGYADFAPLGHSVRVHRDEQKGTIAWKIKFRNGTEKNFLSPIT
                     TKPWGTAIPKLAEVPGPSQEMRDSQLLYNEPKWIRMDDGGLHSLQAAGLKMKKGVYY"
     gene            9987..11855
                     /gene="aprA"
                     /locus_tag="AC16O9_15970"
     CDS             9987..11855
                     /gene="aprA"
                     /locus_tag="AC16O9_15970"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019418 - sulfide oxidation [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HIR6518153.1"
                     /translation="MAYKTIIEDGIDILVVGAGLGGTGAAWEARFWGQDKKIVIAEKA
                     NIDRSGAVAQGLYAINCYMGTRWGENKPEDHVRYARIDLMGMLREDLLFDMARHVDSS
                     VHQFEEWGLPIMKDPKTGRYLREGRWQIMIHGESYKPIVAEAARKSADKIFNRICVTH
                     LLMDDSKENRVAGAVGFNVRTGDYHVFKAKTVICGAGGASNIFKPRSVGEGAGRTWYA
                     PWSSASAYGLMIDAGAKMTQMENRIVLARFKDGYGPVGAYFLHLKTYTQNGLGEEFES
                     KWWPELQKMVGKEYLDPDSFHLTHRPIPTCLRNHALISEVNAGRGPIHMVTMRAFQDP
                     HLEEVGWENFLGMTVGQAVLWAATDVDPKNENPELTTSEPYVMGSHATGSGAWCSGPE
                     DVSPPEYFWGYNRMTTIEGLFGAGDAVGGTPHAFSSGSFTEGRLAAKAACQYIDDGKA
                     EGIVVSDEQIKRRKEEIYKPLEHYRVYRNAIVAGKVNPHYINPEQGLDRLQKLMDEYC
                     GGMTVNYMTNEKLLNIGLKKLKIMEEDLDKLAAEDFHELLRAWELKHRHRAAECVTQH
                     TLFRKETRWPGYYYRGDAMKVDDENWHVLTVSRRDPKTGEYTMEKAPCYHLVSDKE"
     gene            11903..12253
                     /locus_tag="AC16O9_15975"
     CDS             11903..12253
                     /locus_tag="AC16O9_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518154.1"
                     /translation="MNIIDKTDEEILGVAEPLWRDLIKNSNEGKYGEFAKNFSSSLAL
                     AIDQVVAGHQFANSELARSLSADFDALGIIRRGEHVTVLYRQRSSKKPGEWLGRLVLG
                     YEDGKVRIFGASIF"
     gene            12237..12755
                     /locus_tag="AC16O9_15980"
     CDS             12237..12755
                     /locus_tag="AC16O9_15980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6518155.1"
                     /translation="MRPSSERIGDGKFVELTYKVTDKKSGHVLTRVEFPLGYVHGHNE
                     ILAPSVHMALAGKSAGDVIEVTIDGNEIFGPRDESLVVTDRIENVPEEYRQIGTSILM
                     ESDTGQTRSFLVTRMDDATLTVDGNNPLCGREVVFTLEIMTVRDATDEETRAGGAIVA
                     GADVDPSRMRAI"
     gene            12767..12928
                     /locus_tag="AC16O9_15985"
     CDS             12767..12928
                     /locus_tag="AC16O9_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518156.1"
                     /translation="MSEEKKPVTKVPDGHTPFYVTRQLAPTEKGFVGYETVWKPFQKE
                     VEYKTPKKP"
     gene            13067..13993
                     /locus_tag="AC16O9_15990"
     CDS             13067..13993
                     /locus_tag="AC16O9_15990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6518157.1"
                     /translation="MADRRLQVFHAVAKHLSFTKAAEALFMTQPAVTFQIRQLEEHFD
                     TRLFDRAHGRIALTPAGQVALEYAERILGLSAELDTRLKEMSGQVLGPLLIGASTTIA
                     EFLLPQVLGEFKSRYPGVVPRLFVANSEAVQARIAERTLDLGFIEGESHLPSLVTDTC
                     SEDELQVVCAPSHPLAQTGSVAPKMLVEHAYISREPGSGTREVIDRYLQKAGVSPDAL
                     QVVMELGSPEALKGLAATGLGFTIMSRATVAKEIRLGQLVRVPLAPRLTRHLSVVYPK
                     ERFHSRLVNSFVQFAKERFAALQAAEAGSPAA"
     gene            complement(13990..15174)
                     /gene="sat"
                     /locus_tag="AC16O9_15995"
     CDS             complement(13990..15174)
                     /gene="sat"
                     /locus_tag="AC16O9_15995"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HIR6518158.1"
                     /translation="MTGLVKPHGGGALKPLMLAGDALAAERARAATLPRLRVSSREKG
                     DLIMLGIGGFTPLAGFMTRADWEGVCDGYRTAAGLFWPIPITLSTDKATASGIKTGAE
                     IALVDAEDGAVLATMKVTEQYAIDKAHECMSVFRTVDAAHPGVKLVMEQGEVNLAGPV
                     KVLSDGGFKAKYGALFMTPAETRAEFERLGWSRVAAFQTRNPMHRSHEYLAKVAIETC
                     DGVLVHSLLGALKPGDIPAEVRTRAIAVLMEKYFRPGTVVQAGYPLDMRYAGPREALL
                     HALFRQNYGCSHQIVGRDHAGVGSYYGPFDAHRIFDEIPPGALETHPLKMDVTFWCYK
                     CGGMASGRTCAHADADRLQVSGTQLRKWLSEARPVPAEFSRPEVLEVLRAYYTGLEAR
                     PS"
     gene            15282..16949
                     /gene="soxB"
                     /locus_tag="AC16O9_16000"
     CDS             15282..16949
                     /gene="soxB"
                     /locus_tag="AC16O9_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HIR6518159.1"
                     /translation="MQRREFLRLLAMAAAAGASLRASASDTQAASELYDLPAYGNLSL
                     LHMTDAHAQLLPIYYREPSMNLGAGGTASKPPHLVGEALLKHFGIAPGGRAAHAYTYL
                     DFAAAARRYGKVGGYAHLATLVKRVRAARPHALLLDGGDTWQGSATALWTEGADMIGA
                     QQLLGVDLMTAHWEFTYGAARVQRAVKQELGSIEFLAQNVKTLDFEDPVFKPYTLRSV
                     NGVALAVIGQAFPYTPIANPRHMVAEWTFGIQEPRLQALVDEVRAKGAQVVVLLSHNG
                     MDVDLKVAGRVRGLDAILGGHTHDGMPVPVRVGKTLVTNGGASGKFLGVLDLDVKGGA
                     VAGYRYRLLPVFSNLLPADAAMSAYIESVRAPYRAKLEEKLAVTEGLLYRRGTFNGTW
                     DQLLLEALLAEKGAEIAFSPGFRWGSTLLPGDAISFEHLMDQTAITYPYTSLNELTGE
                     QIKAILEDVADNLFNEDPYLQQGGDMVRVGGMTYACNPRADIGARISDMRLAGRPLEA
                     AKRYKVAGWAPVAEGASGEPVWELAARYLRARRTVAPLEAAVPRLSG"
     gene            complement(16939..17568)
                     /gene="soxX"
                     /locus_tag="AC16O9_16005"
     CDS             complement(16939..17568)
                     /gene="soxX"
                     /locus_tag="AC16O9_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR6518160.1"
                     /translation="MNKIGTVATLAAATALLAACAAMQSTPRERATKMMERDFHPVGI
                     AGMDRLKEDAVAAACNKHDDNPPKDVADKLQAQQYAAVKWPADGKFVGNWKEGEKIAQ
                     RGRGMTWRDKPGKTSRGEPNGGSCYNCHQIGPDTTSFGTIGPSLYNFAKLRGYGADIQ
                     RYAYAKIYNAKAYSLCSQMPRFGHSGTLTEEQMKDLTALLMDPASPVNR"
     gene            complement(17579..18397)
                     /gene="soxA"
                     /locus_tag="AC16O9_16010"
     CDS             complement(17579..18397)
                     /gene="soxA"
                     /locus_tag="AC16O9_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR6518161.1"
                     /translation="MRQALMLGIAAAIAAAAPWPQAMAQSEDPVAADFDKFRAIMEEA
                     NPAELFDAKGEELWKKPGGPNKVSLEKCDLGLGPGVVKGAYAQMPRYFADVDRVMDAE
                     ARIVHCMVTLQGYQLDKLDDKPFSSATRTPDPVSITTYVATQSAGMKFAPSQSHPKEK
                     EAYALGKEIFFYRAGPWDFSCASCHALEGKRIRTQPLPNFAKPATALAAVQGWPGYRM
                     TGGVMHTLQWRLNDCFRQQRFPEPGYTSETLSALLTYLTVNAQGQEYKGPGIKR"
     gene            complement(18447..18755)
                     /gene="soxZ"
                     /locus_tag="AC16O9_16015"
     CDS             complement(18447..18755)
                     /gene="soxZ"
                     /locus_tag="AC16O9_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR6518162.1"
                     /translation="MADPMKIRATVQGDVADVRVLMAHPMETGQRSQGGKKVPLHFIQ
                     SITAQLNGKQVFSADISQAISRNPVFAFKVKGAKAGDKISVTWKDNKGESRTDETTVR
                     "
     gene            complement(18769..19233)
                     /gene="soxY"
                     /locus_tag="AC16O9_16020"
     CDS             complement(18769..19233)
                     /gene="soxY"
                     /locus_tag="AC16O9_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR6518163.1"
                     /translation="MLQTRRSVLKAGGALGLLALAGTALLKPGRALAARNASAFEAKS
                     AVDAMKALGMTNAEDSKDIAIRAPDIAENGAVVPIDVTSNIAGTTSISVFIEKNPFPY
                     TGTFDFAEGSVPFVHLRAKMGETSPVRVVVAANGKFYTAVKEVKVTIGGCGG"
     gene            complement(19396..20097)
                     /locus_tag="AC16O9_16025"
     CDS             complement(19396..20097)
                     /locus_tag="AC16O9_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6518164.1"
                     /translation="MKCPECVRLLNAYVDDQLEAAGALAVQEHLRGCARCRTRDESLR
                     TLRDTVHRHTQFRVAPAAMHRALRNEYGARRAAPVQPARRHFAWGAAAMATLALALWG
                     LQGAVERPGAGAGRGAGKVVYHISSSQTASAALRTLRNHLDASPDTKVVVVAHNEGVD
                     FLLRGARDESGQLFQTSVQQFRARGVDFRVCNNTLVRRKIGSDGILPEATLVPSGIAE
                     IGRLQENEGYAYMRL"
     gene            complement(20094..20654)
                     /locus_tag="AC16O9_16030"
     CDS             complement(20094..20654)
                     /locus_tag="AC16O9_16030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6518165.1"
                     /translation="MSDEGKRVRFERLVLPHLDAAYSLAGWLVRDDAQAEEAVQESCL
                     RAFRYFDALRGDEARPWLLGIVRNTCYGLLQRTRQEGPPLSFDEDGCGEDAVAAGAVV
                     SFPLNPEAAAIREADRECVHNALRALPLEFREAIILREIHGFSYREIAATCEVPIGTV
                     MSRIARGRRLLQQALGERGASEDTGT"
     gene            20784..21236
                     /locus_tag="AC16O9_16035"
     CDS             20784..21236
                     /locus_tag="AC16O9_16035"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6518166.1"
                     /translation="MKSIYGLALAGAVGAAMLSGCGTMGADTQATVWPDKVIYHVNEG
                     DDQAVDALRNIKNHLAVNPKAKIVVVTHSKGIAFLKKGARDKNGNPFNVPVEELKERG
                     VQFDLCEITLQRTKQNAKDYLPEITLVPSGVAEITRLQQREGYAYLRP"
     gene            21403..22116
                     /locus_tag="AC16O9_16040"
     CDS             21403..22116
                     /locus_tag="AC16O9_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6518167.1"
                     /translation="MIQIENLVKAFGAKRAVDGVSFAVERGEVLGFLGPNGAGKSTTM
                     RMITGFFPPTSGRVRVGGHDVAEAPLEAKRLIGYLPEAAPSYPDMSVHGFLSFAAEIR
                     GLAGEARRKAVGRVAELCFLGSVLHQSIDTLSKGYRHRTCLAQALIHDPEVLILDEPT
                     DGLDPNQKHEVRNVIRELGRTKAIIFSTHILEEVDAVCTRAIIIDRGRIVASGTPDEL
                     GRPLDEVFRRLTLPDTVKK"
     gene            22113..22847
                     /locus_tag="AC16O9_16045"
     CDS             22113..22847
                     /locus_tag="AC16O9_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6518168.1"
                     /translation="MNAWHAIRTIAKRELASYFASPVAYVFLVIFLLLTGFLTFTAGA
                     FFERGEAGLAAFFAWHPWVYLVLVPAVGMRLWAEERRAGTLELLLTLPVSAWQAILGK
                     FLASWVFLAIALALTFPVVVTVNVLGEPDNGAIAAGYLGSLFLAGAYLSITCLTSAMT
                     RNQVIAFILAVVACLFLILAGFSPVTSLLERWASPALVDTVAAFSVVTHFDGFQKGVI
                     DSKDVIFFLSVIGYALFATGVVLRNR"
     gene            22844..24724
                     /locus_tag="AC16O9_16050"
     CDS             22844..24724
                     /locus_tag="AC16O9_16050"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR6518169.1"
                     /translation="MMRLAKKHEALAYSAVGLAALFLILVAFNYLAAGAAVRADLTAG
                     KLYTLSDGTKRVLRGLPAPVKVKLYITTGEAMPVPVRSFAQRAQSLVEEFKAVAGPNL
                     VIERYDPRPDSEQEDAALLDGIEPQQLMTGEQFYLGVAVSQLDRKQTLPVLAPARERL
                     LEYDLIRAIARAGSAARPKIGLMSGLPVLGEKFNPYTRQSSEPWVLANELRRDFDVQL
                     VNLSATEIDKDLNVLLLIHPRLIGEQTEYALDQFVLRGGKLIAFVDPYAYFDQLPMPP
                     GMPAQVSDSTLPRLFKAWGVSMDPGKVIADVVFASGEGQRYTPTVLSLNRTAFARDDV
                     VTGQIETLLYAFGGAFEVQPAEGLTVTELVRSSPNSMLVDDAKATAAGQETTRSFEAG
                     GKSLPLALRLSGKFRTAFPDGRPEDKAAKPRPEAAAAPAHLAASARDNAVVLVADTDM
                     LQDGAAVDVQEVFGRRIVVPSNGNLAFAQGLVEQLAADESLLSLKTRASAFRPLTVVR
                     QMEAEAQLQYFGKIKELEDELQRTSAALQTLQGGAPEAAKGAQILSPEQQAELERFRK
                     KVAETRAALKDLRKNLRRDSERLVFLTKVANIALMPLIVALAGLAIALVRRRRQARLA
                     AA"
     gene            24721..25791
                     /locus_tag="AC16O9_16055"
     CDS             24721..25791
                     /locus_tag="AC16O9_16055"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR6518170.1"
                     /translation="MNARVAAVLVVLLAVLGGGALLVYQQERARQPAAADALGQPVLR
                     GVPAADIATIVIREPGAQLTLQRREAGWVIAERAGFPADLEQVRGLVIGVLGLKVGAT
                     EPIGDKDRARLRLDGKATQLEFRGADGKALATLLVGKKYFKREPEDAGKAAADGRFVM
                     RPESPNTVVIVADPLPLATAQSASWVAKTGFGAERVRSMEVDLAGAGKWRVERAGLES
                     DWKLIPLYAGEKLDVLRANSATYSLNRVEIVDVAAPGARNEVTGLDKPTTVVASTLDG
                     LTYTIKLGKLRGDEYFVTLWVSGEPKPTGADAAERAKSIAARLPAEKALAGHILLISR
                     SKFEDVLKKRAELLEKKETGKK"
     gene            25838..27904
                     /gene="fusA"
                     /locus_tag="AC16O9_16060"
     CDS             25838..27904
                     /gene="fusA"
                     /locus_tag="AC16O9_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6518171.1"
                     /translation="MGKHAIKHLRTIALVGQAGSGKTSLAEALLARAGAVPAGGSVER
                     GSTVCDYTPLEKQLQHSLKLAVASFEVPNGEAPTVIHLLDTPGYADFLGHALPALAAV
                     ETAAVVINAQNGIEMMTGRFLSWAAHRGLDRLIIVNKIDADNVDLEGLLGEIQEAFGK
                     ECLPLNLPAAKATKVSDCFFAPSGEADFSSVEAAHRNLVDQVVEVDEKLMAEYLEKGE
                     IEAEALHEPLEAAMREGHLIPVVFCSAKTGAGVQELLDVIVKLLPNPAEGNPPVYHGV
                     PADGGAPTEIQATPDPSQHALAHVFKVEIDPYIGRLAVFRVHQGRITPGMQLYVGEGR
                     KPIKPGHLYLLRGKTQTEVSEAVPGDICAIAKVDEIQFDHILHDAAEDAHIHAKPLEL
                     PTSVFGLALQPKKRGDEQKVSDVLHKLVAEDPCLKIEHNAQANETVMRGLGELHLRTT
                     LEKIATQHKLELDTRPPSIPYRETIAARAEGHNRHKKQTGGAGQFGEVFLKVEPLPRG
                     AGFEFVDSVKGGAIPNQFIPSVEKGVRAVLESGYVAGYPIQDVRVIVYDGKSHPVDSK
                     DVAFMSAGRKAFLDALGKARPIVLEPIVNVQIVAPEQKMGDIAGELSGHRGQIKGSDA
                     PRPGTVEITAQAPLSEMEQFPARLKSITAGHGSYSLEFSHYEPAPPQLQQKLISAHRP
                     AASADD"
     gene            complement(27922..28752)
                     /locus_tag="AC16O9_16065"
     CDS             complement(27922..28752)
                     /locus_tag="AC16O9_16065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6518172.1"
                     /translation="MKTVLITGAAGDVGTHLRRELAGKYRIRASDLRELKEKFKGQQV
                     MRADISDFKAALRITKGVDAIIHLGGYSVEGPWEGILSANIIGCYNVFEAARRNGVKR
                     ILFPTSNHAVGFYRRDQTIDHRVYPKPDSRYGVSKVFGEALGSLYADKYGMQVFQIRI
                     GNVNPAPIDKRRLSIWISPRDLAQLVTIGIEHPEIRFEIVYGISGNKRAWYDNANAFR
                     LGYRPQDDSESFAAEVLAREKPGDPVAELYQGGTFVVAEEIPNPAPLPRKRKARKRRG
                     "
     gene            28817..29128
                     /locus_tag="AC16O9_16070"
     CDS             28817..29128
                     /locus_tag="AC16O9_16070"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6518173.1"
                     /translation="MLRALVLAIAFAAAAGAPPARAEATPQVVMYATAWCPYCAKARE
                     YFAESGIAYVEHDVEKSARANAEFKRLGGRAVPLIVVGREKMSGFSEQGFEDMLARAS
                     R"
     gene            29131..30072
                     /locus_tag="AC16O9_16075"
     CDS             29131..30072
                     /locus_tag="AC16O9_16075"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6518174.1"
                     /translation="MTWQPSQRVPDAAVHDVDASFARYRLPLAKIDRLAAGLTRWAEG
                     PVYFGDQRCLVWSDVPGDCMWRWDEETGAVGVWRKPSGHANGNTRDRQGRLVTCEHSG
                     RRVSRTEPDGTVTTLADRFDGKRLNSPNDVVCKTDGSIWFTDPSFGILGWYEGRKAPA
                     ELPTNVYRIDPSGAMSVVAEGINQPNGLAFSPDESILYIVESRSVPRKILACDVVGGK
                     TLSNRRVLIDAGPKGTPDGFRVDVDGNLWCGWGMGEAGLDGVHVFNPDGKLIGRIDLP
                     ERCANVCFGGLHRNRLFMAATTSLYALYVNTQGVANG"
     gene            complement(30069..31121)
                     /locus_tag="AC16O9_16080"
     CDS             complement(30069..31121)
                     /locus_tag="AC16O9_16080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6518175.1"
                     /translation="MRGAVFLGNRKIEIRNFPDPTPGPGEVVLRMRASGMCGSDLKFY
                     RSAPGEAQKALGLGGDAEPFIAGHEPCGEVVARGPGVSEKEAPTGLRVMDHHYAGCGV
                     CPHCRLGWSQLCRAGITVYGVTAHGGHAEYLKVPVRTIVPLPEELSFAEGAAVACGTG
                     TAYGALRRMHVAGGATLAVFGQGPVGISATLLGSKMGLRVIAVEANAERLALAKQYGA
                     DALVDASKTDVEKALKDLTRGEGVDLALDCTGVAAARLQAVRAAKTWGTVCFVGEGGD
                     VTLDVSRDMLRKQLTLIGSWTFSAMGMGECARFVADNKIPLERIFTHRWKLDQADQAY
                     KLFDKQASGKGVIEFS"
     gene            31222..>31506
                     /locus_tag="AC16O9_16085"
     CDS             31222..>31506
                     /locus_tag="AC16O9_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6518176.1"
                     /translation="MFREDLSRNFGFLLNDVARLLRTAYDRRVRKLGLTRAQWWVLTH
                     LFRSNGVSQTELAEILEIEKPTLGRLLDRLEAKRWVRREHDARDRRVWRVH"
BASE COUNT         5104 a        10763 c        10690 g         4949 t
ORIGIN      
        1 tactccacga agatcgacat gcaggagttc aagaaatacc gcggcctgtt cgccgagcaa
       61 ggtgacttcg acatccccga gaactacaac ctcggcgtcg cgttcaaggc caacccgcag
      121 ctgaccatcg ccgccgacta caagcgcatc aactacagca aggtggcctc ggtcggcaat
      181 tcgagcaggc agggcggctg cgcgccgacg atgccggcgg ggccgggcgt cggctccggc
      241 tgtctcggcg cgagcggcgc gagcatcggc ttcggctggc aggacgtgaa cgtgttcaag
      301 atcggcgtcg agtatcagat gaacagcaag ctcctgctgc gtgcgggcta caacctcacc
      361 gacaatccga tcaatgcgag cgacgtgacc ttcaacatcc tcgcgcccgg cgtggtcgaa
      421 gaccacctga cgcttggctt cacgtatgcg ctgcaaggcg gctcggaaat caccatggcc
      481 tatatgcatg cgttcaagaa ctcggtaacc gggccggcga gcaatcctta cttcggtgtc
      541 ggcggtaccg agaccatctc cctcgccgag gattcgctcg gcatttcctg gggaatgaag
      601 ttctaggcgg tgcggccaaa gccgtcctcc tcgacctcga cggcacgctc ctcgacaccg
      661 ccccggacct tgccgcggcg gtgaacgcga tgctcgccgg gcaggggctc aagcccctgc
      721 cggcggccgc gatccgcgac tacatcggca agggcattcc gcacctggtc gagcgctgcc
      781 tcgtcgaggc cgggctgccg ctcacctgcg cgcagctcga gccggcgctg cgcgccttcg
      841 gcgaccacta ccgggcgttc aacggcaaat cgagccggcc gtacccgggc gtcgtcgaag
      901 gtctgcagcg gctgcgcgcc gcgtccctga agctcgcctg cgtcaccaac aaggcgcgca
      961 cgttcacgac gccgctgctc gaggccacag gcctggcgcc ccttctcgac gcggtggtga
     1021 cgagcgacga ggtcggccgg agaaagccgc atccggagcc ctacctgcac gcctgcaagc
     1081 ttctgcaggt cgcgccggag gaagccgtgg tgatcgggga ttccgccaac gatgccgagg
     1141 ccgggcgctc ggcgggatgc cgcgtgctcc tcgtgtccta cggctacagc gaggggcgcg
     1201 atgtgcgcga gctgggcgcg gacgccatcg gggcggaatt tcgagacgcc gtagacctgc
     1261 tgctaggtta atccttccat aagaagtttc tctacaatcc gaggcatggc gtgtccatga
     1321 tcggtgtttt gcagggtaat caccacgaaa gaggagacag acaagatgaa agcgacccgc
     1381 attctcgccg cactgctgct tgctgccgcc gcgctctcgc cgtttgccgt gcacgccgac
     1441 aagggcggac gccatcaaat cgtgatccag gtgaccgaca acgacccggc caagtggaat
     1501 ctcgccctca acgtcgcgca gaatctgcag caggcgtacg gcaaggacaa gaccgacgtc
     1561 gaaatcgtcg cctacggtcc gggcctcaac atgttcaagt ccggctcgac ggtcgcgaag
     1621 cgcctgaacg cggcgcagga cgcaagcgtg gcgctctacg cctgcgagaa cacgatgcag
     1681 aagatgaagg tgaccactgc ggatctgcat cccggttccg tagttgcccc gggcggcggc
     1741 gtcgtccacg tcttcaagcg ccagcgcgag ggttgggaca ccatccgccc gtagccggcg
     1801 tctagcctag cccgacggct tccttcgcga ggccgtcgag cagcgcgtcg acttccttga
     1861 gcgccgcctg cgatgcggcg ctgccgtccg gccgccacgg ccgctggtac gccacatgca
     1921 ccgtcccggg atcctgcgcg gtcgcgtaca cgatgatgcg gtacgggcag aacgcggcgt
     1981 tcgcaggatc ggcctccatg gtcttgcggg agagtgccgc gctgcagaag acgaaggcct
     2041 gcgcgtccgc gtagagtttc ttcgccgagc ccacgtcctt gccggtgcgc tcgagcatgc
     2101 ggccgacgaa cgaatggtag tcaatgacca gtccgcggct ctcgatcgcg agtttcaggt
     2161 cgtcgcgcac atcctcaaag ttcgctcgtt tggagtaact tacgaccgga tgcggatctg
     2221 ccatgggccc ctcccagtga taggtggccc agcgtaggcc cgccgcctgg aggcatcatg
     2281 atccggatca aacgctgcgc cgccgcactg tgccttgcgc tcatcgccgc gccggccgcg
     2341 gccacccacg agccctgggt caccgagcgc ttcccgcagg acgaggcgaa gtggtggtgg
     2401 gacgccgagt ggtgggagcg cggccggatt ccccagacgc ccggccacga ggtggcctcg
     2461 cgcccggcga gctatcgcag cggcgacgtg gaggtcccgg tcgagatcca cgcgccgaag
     2521 ggcgccggcc gctttccggt cgtcgtcttc ctgcacggcc ggcgcgggat cgacccgctg
     2581 acgcgcctcg tgccgctgcg cctcgccgcg cgcggcttca cggtggtcgc gccggacctc
     2641 tacgccggtc gcttcatcgc gcccttcccc atccggcacg acgaggcgct ggaagaggac
     2701 gcggctcgcg cgatcgactt cgcgctcgcg ctcccggagg cgcgcggcca ggcgaagacc
     2761 tgcgtcgttt cgcacacgcg cggcggctat tactcgctca aagccctggt gacccacggc
     2821 cgccaggcga agacggcgtg ctacgcctcc tactacccgc actggcagca ccccgaggcg
     2881 cccgagcccg agcaggtcta ccgctacgcg cgcgaggtcg aggcgctcga agtgccggtg
     2941 ctcgtgttct tcggcgagca cgagcagtac cagcgcgcgc gcccgatcct cgcggggatc
     3001 gaggcgctgc agaaaaaggg ccgcgacgcg cgcgtcctgg tctaccccgg cgtcggccgg
     3061 gggttcgact tccgccctcg cgaagtgcgc accttcgccg acgacctcgc cgcgcaggac
     3121 gcgatgcgcc gcacggcgga attcatgagg aggcatctgc catgaaccct gcacggacgt
     3181 ttcgctggct cgcgctcgcc gcgctgctcg ccagcaccca agccttcgcc caggtcgcca
     3241 aggtggtctg gcacgtggac ttcgcggacg cgcggcgcct gagcgcgatg atccagaacg
     3301 tgaacaacat ggtcaccacc taccagggca acctcgagga ctacgaggtg cacgtcgtgt
     3361 tcctggcggg cggcatccgc ttcgtcacga ccgatcccct gaagggcacg ccgttcgccg
     3421 aggacgaggc gttccgaaag gcgcgccccg acctgatcac gcggctgcag cagctcagaa
     3481 cgctgcacca ggtgaagctc gagctgtgcg agatcacgcg cgagcagctc aacctgccca
     3541 aggacaagat catcgagggc gtggcgccgg tgcgctcggg cgtggtgcgc atcgccgagc
     3601 tgcagtcgaa aggctacgcc tacctcaagg tggactagcc ctcaggcgat cgcctggtag
     3661 tacaggttct gcgggtgctt cgcttcggcg aagaagtacc agcgctcggc gagcaagccg
     3721 gcgaactgca gaaggaacgc cgcgccaggc tggccgtaaa ggagcaatag cgccggcacc
     3781 ggaaacacga gcgcgagcag cgcccagcgc accgcgcgca ccacctcctg cggacggccg
     3841 tggaagaact cgcgcgtgtt gaacgagccg cccatggcgc cttgagcgat ctgcacgatg
     3901 cgcgggtgct tcacgccgat ggcggtggcg agcgtggact tcggccgcag gcgcgtgttg
     3961 cgcgcgagcg cggcgagccg gccgaggtag gccgcggcgc ccacgacgag cgcggcgatg
     4021 acgtagggcc gcgcgagcgg cgcgtgcagc caggcggcga gcgctgcggc cagcgtgagg
     4081 cccgaggcgc tgccgagcag cgtgaagttg acgatcgtga gcggcgtacg ccattcctgc
     4141 aggaacggca ggcaggcgta gatcatcccg gtgcagatga aaagcgcgag cgcgagcacc
     4201 gcgccggccg cgccgagcgc gagcgtcggc tgggcgagcc agtgcgccgc cgcccatgcc
     4261 gcgaccgccg ccatgaacgc gggcagcacg agcacctcgc gcgacagcca cgagctgcgc
     4321 cacctcgccg ccgagcgcca cgcgcgctcg ggacgcccga gatgaaagaa cgaggcgaga
     4381 aggccggcgc cgagcagcgc gagcgatagc gcggcgcccg cgacgaacag ggtcgagccg
     4441 ccgagtccgg cgagctcggc ggcgtagagc gcgaggtaaa gcccctgccc cgcgccgatc
     4501 agcgtcgtca gcaggagcac cgaccaggcg ggccgcatgg cttaccagct cgtcacgtcg
     4561 tcgagcgccg gcgcctgcgg cgacggctgc ggcagcaggc cgtcgatctt gcgcgggttg
     4621 tcgtggcgct cgagctcgtc cgggtgcagg cgcttctcgg tcttcctgcg cggcaggtag
     4681 tggttggcgg ggtgcgtgcc ccactcgggc atcagcgcgt agccgccctg ctcgcggatc
     4741 gcgcgcgagg cttgcgactc gggatcatgg atatcgccga acaggcgcgc gctggtcggg
     4801 caggcgagca cgcacgcggg cttgcgctcg gcctccggca gctgcgcatc gtagatgcga
     4861 tcgacgcaca gggtgcactt cttcatcacc ttctgctgcc cgtccacctc gcgcgcgccg
     4921 taggggcagg cccacgagca gtacttgcag ccgatgcact tgtcgtaatc gacgaggacg
     4981 atgccgtcct cggcgcgctt gtacgacgcg cccgtcgggc acaccggcac gcagggcgga
     5041 tcttcgcaat gcaggcagga cttggggaaa tgcaccgtct cggtcttcgg gtattcgccg
     5101 acctcgaagg tctgcacgcg gttgaagaag gtccctgttg gatcggcgcc gtacggattc
     5161 tggtcggaga gcggccccgc cgcgcctgcc gtgttccact ccttgcagct cgtcacgcag
     5221 gcgtggcagc ccacgcacac gttgaggtcg atcaccagtg cgagctgttt cgtcgtcatg
     5281 cgggggaaat cctcacgcgc acgtcgtacc agccggcctg ccccgtgacc ggatcggagt
     5341 tcgacgtgtc gccgggcagg cggtcggaaa tgagcgagtt caggatgaag ccgcgggtga
     5401 actcgttggc gtcgggggcg aggccccagg cccccgggga cttgccgatc gcgttccagg
     5461 tccacacggt gccgggctcg accgcctcgc tgtgccgccc catgcagcgc acgcgccccc
     5521 actgcgactc gacccacatc cagtcgccgt cgccgatgcc gctcgccgcc gccgtgcgcg
     5581 ggttgatgaa caggtagttg tgcgcgtgga tctgccgcag ccacgcgttc tgcgaatccc
     5641 aggagtggta catggccatc ggccgctgcg tgatcgcggc gagcggatac ttgtcgagat
     5701 cggtggcctg cacctcgagc ggcgcgctgt aaaagggcag cgggtcgaag tgcgtctcga
     5761 cgcgctgcgc aaggtgctcg ggcggccggc ggccgggcca cttgccctgc gcggcgaggc
     5821 gaaagcgctg cagcacctcc gagtacaggt gcaccaggat cggctcgccc cagcgccgca
     5881 gccgcatgcg ctgcgcccac tccatgtagc cgcggttcca gttgcgcatg tagcggtagg
     5941 aaggcgccat ctcgtgcttg aacacgcagt tgttcttcgc gtacatctcc cactgcgcgg
     6001 ggttgggctc gccgagcatc gccttcgcgc cgtcggcgcc gcgccagccg gccaggaaac
     6061 ccatgcccga gccgggctcg gtctcgtggc gcacgatgaa gtccgggtag tcgcggtact
     6121 tgcgcccgcc ttgcgcgtcg gtgaaggcgg ggagcttcag gcggctcgcg agctcgatca
     6181 ggacttcctg gaacggcttg cactcgccct tgggcggcag caccggcacg cgcaccgcgt
     6241 ccacggggcc gtcgaattcc gagatcggcc ggtcgagcat cgacatcacg tcgtggcgct
     6301 cgaggtaggt ggtgtcgggg agcacgaggt ccgcgaacgc cgtggtctcc gactggaacg
     6361 cgtcgcacac gacgagaaag gggatcttgt actcgccgtc ggcgccgcgg tcgttcagca
     6421 tccggcgcac ctcgtccgtg ttcatggtcg agttccacgc catgttggcc atgaagatca
     6481 tcagcgtgtc gagcgggtac gggtcgccgc gccaggcgtt ggtgatcacg ttgtgcatca
     6541 ggccgtgcac cgagagcggg tactcccagg aaaagccctt gtcgatgcgc accgggttgc
     6601 cctcggcgtc gacgaacagg tcctcgggcg atcccggcca gccgagcgcg agcccggcga
     6661 gcggcgtatt gggctgcacg ccctcggggc cggtgggcgt cttcgccgag ggcgggatgg
     6721 cgcgcgggaa cggcgccttg tggcgaaagc ccccgggacg gtcgatggtg cccagaaggc
     6781 tcatcaggat ggcgagcgaa cgcaccgtgt ggaagccgtt cgagtgcgcc gccagcccgc
     6841 gcatggcgtg gaacgccacc gggttgccgg tcaccgactg gtgttcgttg ccccagcagt
     6901 cggtccacgc gatcggcagc tcgattctct cgtcgcgcgc gatcacgccc atctcgtgcg
     6961 ccaggcgccg gatggtcgcc gccgggatcc cggtgatgcc ctcggcccac tcgggcgtat
     7021 gcgcctgcag gcgatcggcg agcagctgga acgcgggccg cacgcgcgtg ccgtcggcga
     7081 gccggtacgc gccaagcagg cgcgggtccg cgcccggcgt gtggctgagc accgcgcgct
     7141 cggaatggcg gtcccaccag agctggttct cgggatacag cgggttctcg ggctcgacat
     7201 cctcgtccac ggcgagcagg ccgtggcgcg gcgaggccgg gtcggcgatc acgagctgtc
     7261 cgccgttggt gtagcgcgcg acgaactcgc ggtcgtacag cccctgcgcg atgagctcgt
     7321 gattgagcgc aagcagcagc gcgccgtcgg tgcccgggcg gatcggcacc cattcgtcgg
     7381 cgatcgccga gtagccggtg cgcaccgggt tcaccgagat gaagcgcccg ccgtcgcgct
     7441 tgaacttcga gatcgcgatc ttgagcggat tggagtggtg atcctcggcg gtgccgagca
     7501 tgacgaagag cctggcgcgc tcgaggtccg ggccgccgaa ctcccagaac gagccgccga
     7561 tcgtgtagat catgccggcg gccatgttca ccgagcagaa tcccccgtgc gcggcgtagt
     7621 tgggcgtgcc gaactggcgc gcaaacaggc cggtgagcgc ctgcatctgg tcgcgcccgg
     7681 tgaacagcgc gaacttcttc gggtccgcgg cgcggatcgc gcccaggcgc gtttccagca
     7741 tcgcgaacgc ctcgtcccag gagatcggct cgaattcgcc cgcgccgcgc gcggcgccgg
     7801 ccttgcgccg cagcggccgc gtgaggcgcg cgggcgagag ctgcttcatg atgccgctcg
     7861 cgcccttggc gcagatcacg cccttgttga gcggatgctc gggattgccg tcgatgtagc
     7921 gcagctcgcc gtcacgcagg tgcacgcgga tgccgcagcg gcacgcgcac atgtagcagg
     7981 tggtggtctt gacctcggcg gacacgtcat gcggattgtc cgcccgcccc cggggcacgg
     8041 caacgagcaa tactgatcct gccatagaat gcgcgcgtga agatctgcat cgtctccgac
     8101 agccacgacc gcgccgacgc gctcgcgcgg gcggtgcgcg aagcgaaagc cgcaggcgcc
     8161 gaggcggtga tccactgcgg cgacctgatc ggggcacaga ccgtgaagcc ggcgctcgcg
     8221 gccgggctgc cggtgcacct catccacggc aacaacatcg gcgacacgca ggcgctgcac
     8281 cgccagtcgc gcatgagcgg cgggcagctg cagtatcacg gggccgacgc gcgcctcgag
     8341 ctcggcagcc ggcgcgtgtt cgtcgtgcac tacgacgact acggctacgc catggcctgc
     8401 accggcgagt gggacctggt gtgctgcggg cactcgcaca aggccgaggc gcgcgaggtg
     8461 acgggcgtga atggcgggcg cacctggctc gtcaatcccg gcacggtcgc cggcctgtcg
     8521 gccccggcga cctggctgct gggcgatctc gcgtcgatgc gcttcgagat ccgcgcattg
     8581 aacgcctcta taagcgcata gcgaactcta agccgggcgc ctaggggcgg cgcttagaat
     8641 tcccgcaggg aagccgagga ggcgaagtca tgttcaccag caatccgttt gccacgctct
     8701 cggcgtcggt gccggccgag ttcatgcaag gctttgtgct cctgatgctc gtcctggtcg
     8761 cggcgggcac gctgttcgag gtcgtgcaca agggcagcgc gaagtacttc ttccagaact
     8821 ggcgcaactc gcgcagcaag gcggcgaagc cgctcggcgg cggcgagctc gtcgccatcg
     8881 cggtgaaaac ggcgctggtg gacgtcgcgg cctccggcga gttctgcaac atgcggcgcc
     8941 gcgtggcgca tctgctcacg atgtacggct tcgtgctcta cgtgatcgcg accgccgtga
     9001 tggtgttcga cctgtcggcg cgcggcctgg tggcgcagct gtggtggctg ggcggcctga
     9061 tggtgctcgc cggcggctac tggttctggt tcttcatccg cgtcgacgtc gccgccgagg
     9121 gcaactcgcc gctgcgcgtc gtgtacgccg atgtcttcat cctgtcgctg ctggtgagcg
     9181 tgaccctcgg cctcgtctgg gcgggtgccg gcggcggcgc cggcgtgctc ttcgcgctct
     9241 acgtcctcac cacggcggtg ctgttcggct cggtgccgtg gtccaagttc gcgcacatgt
     9301 tcttcaagcc ggcggcggcc ttcgagaaga gggtttccaa cgcgaatggc tcggccgaga
     9361 acctgccgac gctgacccgg gacgatcccg agcagcagaa gagacactcc atggatctgc
     9421 tgcgggacgc gccgatggac atggggctcg gcatcaagcg cgaagcgccc cgtcactact
     9481 gagaagcagg aacaaggagc cggactcatg cctaccttcg tctacatgac aaggtgcgac
     9541 ggatgcggac actgcgtcga catctgtccc tcggacatca tgcacatcga caagacctat
     9601 cggcgcgcct acaacatcga gccgaacatg tgctgggagt gctactcctg cgtgaaggcg
     9661 tgcccgcagc atgcgatcga cgtgcgcggc tacgccgact tcgcgccctt gggccacagc
     9721 gttcgcgtcc accgcgacga acagaaaggc acgatcgcgt ggaagatcaa gttccgcaac
     9781 ggcacggaaa agaacttcct gtcgcccatc accaccaagc cctgggggac ggcgattccc
     9841 aagctcgccg aggttcccgg gcccagccag gagatgcgcg acagccagct cctgtacaac
     9901 gagccgaaat ggatccgcat ggatgacggc ggtctacatt cgcttcaggc cgcgggtctc
     9961 aagatgaaga aaggcgtgta ctactgatgg cctacaagac gatcatcgaa gacggcatcg
    10021 acatcctggt cgtcggtgcg ggcctgggcg gcaccggcgc cgcctgggag gcgagattct
    10081 ggggacagga caagaagatc gtcatcgccg agaaggccaa catcgatcgc tcgggtgcgg
    10141 tcgcccaggg cctgtacgcc atcaactgct acatgggcac ccgctggggc gagaacaagc
    10201 ccgaggacca cgttaggtac gcgcgcatcg acctgatggg gatgttgcgg gaagacctgc
    10261 tgttcgacat ggcccgccac gtcgactcct cggtgcacca gttcgaggag tggggcctgc
    10321 ccatcatgaa ggacccgaag accgggcgct atctgcgcga ggggcgatgg cagatcatga
    10381 ttcacggcga gtcctacaag cccatcgtcg ccgaggccgc caggaaatcg gcggacaaga
    10441 tcttcaaccg catctgcgtc acccacctgc tgatggacga cagcaaagag aaccgggtcg
    10501 ccggtgccgt cggcttcaac gtacgcacgg gcgactacca cgttttcaag gcgaagaccg
    10561 tgatctgcgg tgcgggcggt gcctccaaca tcttcaagcc gcgttcggtg ggtgaaggcg
    10621 ccggccgcac ctggtatgca ccgtggtcgt cggcgtccgc ctatgggctg atgatcgacg
    10681 ccggcgccaa gatgacgcag atggaaaacc gcatcgtgct ggcgcgcttc aaggacggct
    10741 atggtccggt cggcgcctac ttcctgcacc tgaagaccta cacgcaaaac ggcctgggcg
    10801 aagaattcga gtccaagtgg tggcccgagc tccagaagat ggtcggcaag gaatacctgg
    10861 atccggattc cttccacctg acccatcggc cgatcccgac gtgtttgcgc aaccacgcat
    10921 tgatcagcga ggtcaacgcc ggccgcggcc ccattcacat ggtcaccatg cgcgccttcc
    10981 aggatcccca tctggaagag gtcggctggg agaacttcct cggcatgacg gtcggccagg
    11041 cggtgctctg ggccgccacc gacgtcgatc ccaagaacga gaatcccgag ctgaccacct
    11101 ccgagcccta cgtcatgggc tcgcacgcga ccgggtcggg tgcctggtgc tccggtccgg
    11161 aggacgtttc cccgcccgag tatttctggg gctacaaccg catgacgacg atcgaaggcc
    11221 tgttcggcgc cggcgacgcc gtcggcggca cgccgcacgc cttctcctcc ggctctttca
    11281 ccgagggtcg tctcgccgcg aaggccgcct gccagtacat cgacgacggc aaggccgaag
    11341 gcatcgtcgt atccgacgag cagatcaagc gccgcaagga agagatctac aagcccctgg
    11401 agcactaccg ggtctaccgc aacgcgatcg tggcgggcaa agtcaacccg cactacatca
    11461 atcccgagca gggcctggac cgcctgcaga agctgatgga cgagtattgc ggtggcatga
    11521 ccgtcaacta catgaccaac gagaagcttc tgaacatcgg cctgaagaag ctcaagatca
    11581 tggaggaaga cctcgacaaa ctggccgcgg aggatttcca cgagttgctg cgggcgtggg
    11641 aactgaagca ccgccatcgc gcggcggagt gcgtcacgca gcacacgctg ttccgcaagg
    11701 agacccgctg gccgggctac tactaccgtg gcgacgccat gaaggtggac gacgagaact
    11761 ggcacgtgct cacggtgtcg cgtcgcgacc ccaagaccgg cgaatacacc atggagaagg
    11821 cgccttgcta tcacctggtt tcggacaagg agtagcagcg tccccgatgc cgccggcccc
    11881 ttccgttcgc ggagagccgc gcgtgaacat catcgacaag acggacgaag agatcctggg
    11941 cgtcgccgaa ccgctgtggc gcgacctcat caagaattcg aacgagggca agtacgggga
    12001 attcgcgaag aacttctcca gttccctggc gctcgccatc gatcaggtgg tggcggggca
    12061 ccagttcgcc aacagcgagc tcgcgagaag cctgtccgcg gacttcgacg cgctcggcat
    12121 catccgccgg ggcgagcatg tgacggttct ttaccggcag cgcagcagca agaagccggg
    12181 cgaatggctc ggccggctgg tcctcggtta tgaggacgga aaggtcagga ttttcggtgc
    12241 gtccatcttc tgagcgcatc ggcgacggca agttcgtcga gctcacctac aaggtgaccg
    12301 acaaaaagtc ggggcatgtt ctcacccggg tcgaattccc gttgggctat gttcacggac
    12361 acaacgagat tctggctcct tccgtccaca tggcgctcgc cggcaagtcc gccggcgacg
    12421 tcatcgaagt gacgatcgat ggcaatgaga tcttcggccc cagggacgag tcgctggtcg
    12481 ttaccgatcg catcgagaac gtccccgagg agtatcggca aatcggcacc tccatactca
    12541 tggaaagcga cacgggccag acccgcagct ttctcgtgac gcggatggac gacgcgacgc
    12601 tgacggtcga tggcaacaat ccgctctgcg gccgagaggt cgtcttcacg ctcgagatca
    12661 tgactgtgcg ggacgcgacc gatgaagaaa cgagagcggg cggggcgatc gtggcgggcg
    12721 cggatgtcga cccgtcacgc atgagggcaa tctgaagagg tgatgcatga gcgaggaaaa
    12781 gaagccggtt accaaggttc ctgatggcca cacgcctttt tatgtgacgc gccagctggc
    12841 gccgaccgaa aaggggtttg ttggttacga gaccgtctgg aaaccgttcc agaaggaagt
    12901 cgagtacaag acgcccaaga aaccgtaggc gtcataccgc cactcttatg agccccgcag
    12961 gggaaagccc gcggcggggg tcataagtca cccccggggc ggcctttatc gatgcccggg
    13021 tttatgcttc aatggcattt ccttatccac tgtcggtggc gcagtcatgg cggaccgtcg
    13081 cctgcaagtc ttccacgccg tcgcgaagca cctgtccttc accaaggcgg ccgaggcgct
    13141 gttcatgacg cagccggcgg tgacgttcca gatccgccag ctcgaggagc acttcgacac
    13201 gcgcctgttc gaccgcgcgc acgggcgcat cgcgctcacc cccgccggcc aggtggcgct
    13261 cgagtacgcc gagcgcatcc tcggcctttc cgccgagctc gacacgcggc tgaaggagat
    13321 gagcggccag gtgctggggc ctttgctcat cggcgccagc accaccatcg ccgagttcct
    13381 gctgccgcag gtgctgggcg aattcaagtc gcgctacccg ggcgtggtgc cgcgcctgtt
    13441 cgtcgccaac tcggaagccg tgcaggcgcg catcgccgag cgcacgctcg acctcggatt
    13501 catcgagggc gagtcgcacc tgccctcgct ggtgaccgat acgtgctcgg aggacgagct
    13561 gcaggtggta tgcgcgcctt cgcacccgct cgcccaaacg ggctcggtcg cgccgaagat
    13621 gctggtcgag cacgcctaca tcagccgcga gccgggctcg ggcacgcgcg aggtgatcga
    13681 ccgctacctg caaaaggcgg gggtgtcgcc cgatgcgctg caggtggtga tggagctggg
    13741 cagccccgag gcgctgaagg gcctcgccgc caccgggctc ggattcacga tcatgtcgcg
    13801 cgccacggtg gccaaggaga tccggctcgg ccagctggtg cgcgtgccgc tcgcgccgcg
    13861 gctcacccgg cacctgtcgg tggtctatcc caaggagcgc ttccactcgc gactggtgaa
    13921 cagcttcgtg cagttcgcca aggagcgctt cgcggcgctg caggctgctg aagccgggag
    13981 cccggccgcc tagctcggtc gagcttccag gccggtgtag taggcgcgca gcacttcgag
    14041 cacctcgggg cggctgaact cggccggcac cgggcgagcc tccgagagcc acttgcgcag
    14101 ctgcgtgccc gagacctgca ggcgatcggc gtcggcgtgg gcgcaggtgc gccccgaggc
    14161 catgccgccg cacttgtagc accagaaggt cacgtccatc ttgagcgggt gcgtctcgag
    14221 cgcgcccggc gggatttcgt cgaagatgcg atgcgcgtcg aacgggccat agtagctgcc
    14281 cacgccggcg tggtcgcgcc cgacgatctg gtgcgagcag ccgtagttct ggcgaaacag
    14341 cgcgtgcagc agcgcctcac gcggccccgc gtagcgcatg tccaggggat agcccgcctg
    14401 caccacggtg ccggggcgga agtacttctc catcagcacg gcgatggcgc gcgtgcgcac
    14461 ctcggccggg atgtccccgg gcttgagcgc gccgagcagc gagtgcacca gcacgccgtc
    14521 gcaggtctcg atcgccacct tggcgaggta ctcgtgcgag cggtgcatcg ggttgcgggt
    14581 ctggaacgcg gcgacgcgcg accagcccag ccgctcgaac tcggcacgcg tctcggccgg
    14641 cgtcatgaag agcgccccgt acttcgcctt gaacccgccg tccgaaagca ccttcaccgg
    14701 cccggcgagg ttcacctcgc cctgctccat caccagcttc acgcccgggt gtgcggcgtc
    14761 cacggtgcgg aacaccgaca tgcactcgtg cgccttgtcg atcgcgtact gctccgtcac
    14821 cttcatggtg gcgagcacgg cgccgtcctc agcgtcgaca agcgcgatct cggcacccgt
    14881 cttgatgccg ctggccgtcg ccttatccgt ggacagcgtg atcggaatgg gccagaacag
    14941 gcccgccgcc gtgcgatagc cgtcgcacac gccttcccag tcggcgcgcg tcatgaaacc
    15001 ggcgagcggc gtgaagccgc cgatgccgag catgatgagg tcgcccttct cgcgcgagct
    15061 cacgcgcagc ctgggcagcg tggcggcgcg cgcgcgctcg gcggcgagcg cgtctcccgc
    15121 gagcatcagc ggcttgaggg cgccgccgcc gtgcggcttg accagacctg tcatgcgggc
    15181 attgtccgta cccgcgccgc cgccgggcaa ctgcgtccta ttgatccgcc aataaggttg
    15241 gctgatccac ggcgacccct tcgctcatgc agaatgcgca catgcaaaga cgcgaattcc
    15301 tccgcctgct cgcgatggcg gctgctgccg gtgcatcgct gcgcgcgagc gcgagcgaca
    15361 cacaggcggc gagcgagctc tacgatctgc cggcctacgg caacctgtcg ctgctgcaca
    15421 tgaccgacgc gcacgcgcag ctgctgccga tctactaccg cgagccgagc atgaatctgg
    15481 gcgccggggg caccgccagc aagccgccgc acctcgtggg cgaggcgctg ctcaagcatt
    15541 tcggcatcgc tcccggcggg cgcgccgcgc acgcctacac ctacctcgat ttcgccgccg
    15601 ccgcgcgccg ctacggcaag gtcggcggct acgcgcatct ggcaaccctg gtgaagcgcg
    15661 tccgcgcggc gcgcccgcac gcgctgcttc tggatggcgg cgacacctgg cagggctcgg
    15721 ccacggccct atggaccgag ggcgccgaca tgatcggcgc gcagcagctg ctcggcgtgg
    15781 atctgatgac cgcgcactgg gagttcacct acggcgccgc gcgcgtgcag cgcgcagtga
    15841 agcaggagct cggctcgatc gagttcctgg cgcagaacgt gaagacgctc gacttcgagg
    15901 atccggtctt caagccctac acgctgcgct ccgtgaacgg cgtcgcgctg gccgtcatcg
    15961 gccaggcctt tccctacacg ccgatcgcca atccgcgcca catggtcgcc gagtggactt
    16021 tcggcatcca ggagccgcgg ctgcaggcgc tcgtggacga ggtgcgcgcc aagggggcgc
    16081 aggtggtggt gctcctgtcg cacaacggca tggacgtgga cctcaaggtc gcgggccgcg
    16141 tgcgcgggct cgatgccatt ctcggcggcc acacgcacga cggcatgccg gtgcccgtgc
    16201 gcgtcggcaa gaccttggtc accaacggcg gcgcgagcgg gaagtttctc ggcgtgctcg
    16261 acctggacgt gaaggggggc gcggtcgccg gctaccgcta ccggctgctg ccggtgttct
    16321 cgaacctgct cccggccgac gccgcgatga gcgcctacat cgagagcgtg cgcgcgccgt
    16381 accgcgcgaa gctggaagag aagctcgccg tcaccgaggg actgctgtat cggcgcggca
    16441 ccttcaacgg cacctgggac cagctgctgc tcgaggcgct gctcgccgag aagggcgccg
    16501 agatcgcgtt ctcgccggga ttccgctggg gcagcacgct gctgccgggc gacgcgatca
    16561 gcttcgagca cctgatggac cagaccgcga tcacctatcc ctatacgagc ctgaacgagc
    16621 tcaccggcga gcagatcaag gcgatcctcg aggacgtggc ggacaacctg ttcaacgaag
    16681 acccgtacct gcagcagggc ggcgacatgg tgcgggtggg cggcatgacc tacgcctgca
    16741 atccgcgcgc cgacatcggc gcgcgcatct ccgacatgcg cctcgccggc cgcccgctcg
    16801 aggcggcgaa gcgctacaag gtggcgggct gggcgccggt cgccgagggc gcgagcggcg
    16861 agccggtgtg ggagctcgcc gcgcgctacc tgcgcgcgcg gcgcaccgtc gcgccgctcg
    16921 aagcggccgt gccgcggctc agcggttgac cggcgaagcc gggtccatca gcagcgcggt
    16981 caggtccttc atctgctcct cggtgagcgt gcccgagtgg ccaaagcgcg gcatctggga
    17041 gcacaggctg taggccttcg cgttgtagat ctttgcgtac gcatagcgct ggatgtccgc
    17101 gccgtagccg cgcagcttgg cgaagttgta cagactcggg ccaatcgtgc cgaacgacgt
    17161 cgtatcgggg ccgatctggt ggcagttgta gcagctgccc ccgttcggct cgccgcgcga
    17221 ggtcttgccc ggcttgtcgc gccaggtcat gccgcggccg cgctgcgcga tcttctcgcc
    17281 ctccttccag ttgccgacga acttgccgtc ggcgggccac ttgaccgcgg catattgctg
    17341 cgcctgcagc ttgtcggcca cgtccttcgg cgggttgtcg tcgtgcttgt tgcaggcggc
    17401 ggcaaccgcg tcttccttca ggcggtccat gccggcgatg cccaccgggt ggaagtcacg
    17461 ctccatcatt ttcgttgccc gctctcgcgg cgtcgactgc atggccgcgc aagcggcgag
    17521 cagcgccgtg gcggcagcaa gcgttgcgac ggtaccgatc ttgttcatgg cgcctcccct
    17581 agcgcttgat gccggggccc ttgtactcct ggccctgggc gttgacggtt aggtacgtga
    17641 gcagcgcgct cagcgtctcc gaggtgtagc ccggctcggg gaagcgctgc tggcggaagc
    17701 agtcgttcag gcgccactgc agggtgtgca tcacgccacc cgtcatgcgg tagcccggcc
    17761 agccctgcac cgcggcgagc gccgttgcgg gcttcgcgaa gttgggcagc ggctgggtgc
    17821 ggatgcgctt gccctcgagc gcatggcaac tcgcgcagga gaagtcccaa gggcccgcgc
    17881 ggtagaagaa gatctccttg ccgagcgcgt aggcttcctt ctccttcgga tgcgactgcg
    17941 acggcgcgaa cttcatgccc gcagactgcg tggcgacgta ggtcgtgatc gacaccgggt
    18001 ccggggtgcg cgtggcgctg ctgaagggct tgtcgtcgag cttgtccagc tggtagccct
    18061 gcagcgtcac catgcaatgc acgatgcgcg cctcggcgtc catgacgcga tcgacgtcgg
    18121 caaagtagcg cggcatctgc gcgtaggcgc ccttgacgac gcccggtccg aggccgaggt
    18181 cgcacttctc gagcgaaacc ttgttcggcc cgccaggttt cttccacagc tcctcgccct
    18241 tggcgtcgaa cagctcggcc gggttggctt cctccatgat ggcgcggaac ttgtcgaagt
    18301 ccgcggcgac cggatcctcg ctctgcgcca tcgcctgcgg ccagggcgcg gccgcggcaa
    18361 tcgctgcggc aatacccagc atgagcgctt gtctcatctt cacctcctcc atttcgatcg
    18421 aacggggctt ctgcggcccc gggccgtcag cgtaccgtcg tctcgtcggt gcgcgattcg
    18481 cccttgttgt ccttccaggt caccgaaatc ttgtcgccgg ctttcgcgcc cttcaccttg
    18541 aacgcgaaca ccgggttgcg cgagatcgcc tggctgatgt cggcgctgaa cacctgcttg
    18601 ccgttcagct gcgcggtgat gctctggatg aaatgcagcg gcaccttctt gccgccctgg
    18661 ctgcgctggc cggtctccat cgggtgcgcc atcaggacgc gcacgtcggc gacgtcgccc
    18721 tgcacggtgg cgcggatctt catcgggtct gccatggtcg tcttctcctc agccgccgca
    18781 gccgccgatg gtgaccttca cttccttaac ggcggtatag aacttgccgt tggcagccac
    18841 caccacgcgc accgggctgg tctcgcccat cttggcgcgc aggtgcacga aggggaccga
    18901 gccttccgcg aagtcgaagg tgcccgtgta cgggaacggg ttcttctcga tgaacacgga
    18961 aatgctggtg gtgccggcga tattgctcgt gacgtcgatc ggcaccacgg cgccgttctc
    19021 ggcgatgtcc ggcgcgcgga tcgcgatgtc cttgctgtcc tcggcgttcg tcatgccgag
    19081 cgctttcatc gcgtccacgg cagacttcgc ctcgaaagcg ctggcgtttc gggccgcgag
    19141 ggcgcgcccg ggcttgagca gggcggtccc ggccaatgcg agcagcccaa gtgcgccgcc
    19201 cgccttcaat acggatctgc gagtctgtag catgcttcct cctcctgccc ggtttgacgt
    19261 gtagtaatga aaagcctggg ctcggtcccc aagcctactc aaatgtccct gcggcgcaca
    19321 agcttcatga ccagacgcac gtcaaggaaa tttgatttgc cccataacga cagctaatcc
    19381 tagcgcggcg cgcgctcaca ggcgcatata cgcatagcct tcgttctcct gcaggcgccc
    19441 gatttccgcg atacccgagg gtaccagcgt cgcttcgggc aggatgccgt cgctgccgat
    19501 cttgcgtcgc acaagcgtgt tgttgcacac gcggaaatcc acgccgcgcg ccctgaactg
    19561 ctgcaccgac gtctggaaca gctggcccga ttcgtcgcgg gcgccgcgca gcaggaagtc
    19621 gacgccctcg ttgtgcgcca ctaccacgac tttcgtgtcc ggcgaggcgt caaggtggtt
    19681 gcgcagcgtg cgcagcgccg cgctcgcggt ctggctgctc gagatgtggt agaccacctt
    19741 gcccgcgccg cggccggcgc cggctcccgg tcgctcaacc gcgccttgca gcccccagag
    19801 cgccagcgca agcgtggcca ttgctgcggc gccccaggcg aagtggcgac gcgccggctg
    19861 caccggggcg gcacggcgcg cgccatattc gttgcgcagc gcgcggtgca tggccgccgg
    19921 cgcgacgcgg aactgggtgt gccggtgcac ggtgtcgcgc agcgtgcgca ggctctcgtc
    19981 gcgggtacgg cagcgcgcac agcctcgcag gtgctcctgc accgcgagcg cgcccgccgc
    20041 ctcgagctga tcgtcgacgt acgcgttcag gagccggaca cattcagggc acttcacgtg
    20101 ccggtatcct cgcttgcgcc gcgctcgccg agcgcctgct gcagcagcct gcgcccgcgc
    20161 gcgatgcgcg acatgacggt gccgatcggc acttcacagg tggccgcgat ttcgcgatag
    20221 ctgaagccgt ggatttcgcg caggatgatc gcctcgcgga attccagcgg cagcgcgcgc
    20281 agggcgttgt gcacgcactc gcggtcggct tcgcggatcg ccgccgcctc gggattgagc
    20341 ggaaagctga ccacggcgcc cgcggccacg gcgtcctcgc cgcaaccgtc ctcgtcgaag
    20401 gacagcggcg gaccctcctg gcgggtgcgc tgcagcaggc cgtagcaggt attgcggacg
    20461 atgccgagca gccacggccg cgcctcgtcg ccgcgcaggg cgtcgaagta gcggaacgcg
    20521 cgcaggcagg attcctgcac ggcttcctcg gcctgggcgt catcgcgcac cagccaccct
    20581 gcaaggctgt aggcggcgtc gagatgcggc agcaccagac gctcgaaccg cacgcgcttt
    20641 ccctcgtcgg acaaagcccc attcccccaa ataccgcgcc cgcgtcgatt ctattccagg
    20701 gccggcggcg ggaatattcc ggcgcccgca ccggtatgga ggcggcaggg ctctaatccc
    20761 gatacaacac aaggaggaga acaatgaaat cgatttatgg gctggcactg gcgggggccg
    20821 ttggcgcggc gatgctgtcg ggttgcggca cgatgggtgc cgacactcag gcgaccgtct
    20881 ggccggacaa ggtgatctac cacgtgaacg agggcgacga tcaggcggtt gacgcgctgc
    20941 gcaacatcaa gaaccacctc gcggtcaacc ccaaggcgaa gatcgtcgtg gtgacgcact
    21001 ccaagggcat cgccttcctg aagaagggag cgcgcgacaa gaacgggaac ccctttaatg
    21061 tcccggtgga agagcttaag gagcgcggcg tgcagttcga cttgtgcgag atcacgctcc
    21121 agcgtaccaa gcagaacgcc aaggactacc tgcccgagat cactctcgtg ccctccgggg
    21181 tggccgagat cacccgcctg cagcagcgcg agggctacgc ctacctgcgg ccctgagcag
    21241 cgtccccggc ggtggcggcg gcacgccgcc gccggggcgg cagccgggga ggatcgggac
    21301 acccctggcg cgggccgggc gcagccggct atttcggcga taatgcgccc ccttgaagtt
    21361 tcccggcgcg ccgcctaaat gacgcccggg gcggaaatcc cgatgatcca aatcgaaaac
    21421 ctggtgaagg ctttcggcgc caagcgcgcc gtggacggcg tgtccttcgc cgtcgagcgc
    21481 ggcgaggtgc tcggctttct cggcccgaac ggcgccggca agtcgaccac catgcgcatg
    21541 atcacgggct ttttcccgcc gaccagcggc cgcgtccggg tcggcggcca cgacgtggcc
    21601 gaggcgccgc tcgaggcaaa gcgcctgatc ggctacctgc ccgaggccgc gccgtcctac
    21661 cccgacatga gcgtgcacgg gtttctttca ttcgccgccg agatccgcgg gctggcggga
    21721 gaagcgcgcc gcaaggccgt gggccgcgtg gccgagctgt gctttctcgg ctcggtgctg
    21781 caccagtcga tcgacacgct gtccaagggc taccggcacc gcacctgcct tgcgcaggcg
    21841 ctgatccacg atcccgaggt gctgatcctc gacgagccca ccgatggcct cgacccgaac
    21901 caaaagcacg aggtgcggaa cgtgatccgc gagctcggca ggaccaaagc gatcatcttc
    21961 tcgacgcaca tcctcgagga ggtcgacgcc gtctgtaccc gcgccatcat catcgaccgc
    22021 ggccgcatcg tggcgagcgg cacgcccgac gagctcggcc ggccgctcga tgaggtgttc
    22081 cgccgcctca cgctccccga cacggtgaag aaatgaacgc ctggcacgcc atccgtacca
    22141 tcgccaagcg cgaactggcc tcgtacttcg cctcgcccgt ggcctacgtg ttcctcgtca
    22201 tcttcctgct gctgaccggc tttctcacct tcaccgccgg cgcgttcttc gagcgcggcg
    22261 aggcgggcct cgccgcgttc ttcgcctggc atccgtgggt gtacctggtg ctggtgccgg
    22321 cggtcggcat gcgcctgtgg gccgaagagc ggcgcgcggg gacgctcgag ctgctgctca
    22381 cgctgccggt gtccgcctgg caggcgatcc tcggcaagtt cctcgcttcc tgggtgttcc
    22441 tcgccatcgc cctcgcgctg accttcccgg tggtggtgac cgtgaacgtg ctcggcgagc
    22501 ccgataacgg ggccatcgcc gccggctatc tcggcagcct gttcctcgcc ggcgcgtatc
    22561 tctccatcac ctgcctgacc tcggcgatga cgcgcaacca ggtcatcgcc ttcatcctcg
    22621 ccgtggtcgc gtgcctgttc ctgatcctcg ccggcttcag cccggtgacg agccttcttg
    22681 agcgctgggc gagcccggcc ctggtcgaca cggtcgccgc gttctcggtg gtcacgcact
    22741 tcgacggctt ccagaagggc gtcatcgact cgaaggacgt gatcttcttc ctctcggtga
    22801 tcggctacgc gctgttcgcc accggcgtgg tgttgcgcaa ccgatgatgc ggctggcgaa
    22861 gaagcacgaa gcgctcgcct actccgcggt ggggctcgcg gcgctgttct tgatcctggt
    22921 cgcgttcaac tacctcgccg cgggcgccgc ggtgcgcgcc gacctcaccg cgggcaaact
    22981 ctacacgctc tcggatggca cgaagagggt gctgaggggc ctgccggcgc cggtgaaggt
    23041 caagctctac atcacgacgg gcgaggccat gccggtgccg gtgcgcagct tcgcgcagcg
    23101 cgcgcagagc ctggtcgaag agttcaaggc cgtcgccggc ccgaacctgg tcatcgagag
    23161 atacgatccg cggcccgaca gcgagcagga ggatgcggcg ctgctcgacg gcatcgagcc
    23221 acagcagctg atgacgggcg agcagttcta cctcggcgtg gcggtgagcc agctcgatcg
    23281 caagcagacc ctcccggtgc tcgccccggc gcgcgagcgg ctgctcgagt acgacctgat
    23341 ccgcgccatc gcccgcgccg gcagcgccgc gcggccgaag atcgggctga tgagcgggct
    23401 gccggtgctg ggcgagaagt tcaaccccta cacccgccag agctccgagc cctgggtgct
    23461 cgccaacgag ctgcgccgcg atttcgacgt gcagctggtc aacctgtcgg cgaccgagat
    23521 cgacaaggac ctcaacgtcc tgctgctcat ccatccgcgg ctgatcggcg agcagaccga
    23581 gtacgcgctc gaccagttcg tgttgcgcgg cggcaagctc atcgccttcg tcgacccgta
    23641 cgcctacttc gaccagctgc cgatgccccc gggcatgccg gcgcaggttt ccgactcgac
    23701 gctgccgcgc ctgttcaagg cctggggcgt gagcatggac ccgggcaagg tgatcgccga
    23761 cgtggtgttc gcctccggcg aggggcagcg ctacacgccg acggtgctgt cgctcaaccg
    23821 caccgcgttc gcccgcgacg acgtggtgac gggccagatc gagacgctgc tctacgcctt
    23881 tggcggcgcc ttcgaggtgc agcccgccga ggggttgacg gtcaccgagc tggtgaggag
    23941 ctcgcccaat tccatgctgg tcgacgacgc caaggccacg gccgcgggcc aggagacgac
    24001 gcgcagcttc gaggccggcg gcaagagcct gccgctcgcg ctgcgccttt ccggcaagtt
    24061 ccgcaccgcg ttccccgacg gccggcccga agacaaggcg gccaagccgc ggcccgaggc
    24121 cgctgccgcg cccgcgcacc tggccgcgtc ggcgcgcgac aacgccgtgg ttctggtcgc
    24181 cgataccgac atgctgcagg acggcgccgc ggtggacgtg caggaagtct tcggccggcg
    24241 catcgtcgtg ccctcgaacg gcaacctcgc cttcgcgcag ggcctggtgg agcagctcgc
    24301 cgccgacgag agcctgctgt cgctgaaaac ccgcgcgtcc gccttccgcc cgctcacggt
    24361 ggtgcgccag atggaagccg aggcgcagct gcagtacttc ggcaagatca aggagctcga
    24421 ggacgagctg cagcggacca gcgcggcgct gcagacgctg cagggcggtg cgcccgaggc
    24481 ggccaagggc gcgcagattc tctcgcccga gcagcaggcc gagctcgagc gcttccgcaa
    24541 gaaggtggcc gagacgcgcg cggcgctgaa ggatctgcgc aagaacctgc ggcgcgattc
    24601 cgagcgcctc gtgttcctca ccaaggtggc caacatcgct ttgatgccgc tcatcgtggc
    24661 gctcgcgggc ctcgccatcg cactcgtgcg gcggcggcgc caggccaggc tggctgcggc
    24721 atgaacgcgc gtgttgccgc ggtgctggtg gtgctgctcg ccgtactggg cggcggcgcg
    24781 ctgctcgtgt accagcagga gcgcgcgcgc cagcccgcgg ccgcggacgc gctgggccag
    24841 cccgtgctgc gcggcgtgcc ggccgcggac atcgcgacca tcgtgatccg ggagcccggc
    24901 gcccagctca ccctgcagcg gcgcgaggcc ggctgggtga tcgccgagcg cgccgggttt
    24961 cccgccgacc tcgagcaggt gcgagggctg gtgatcgggg tgctcggcct caaggtcggc
    25021 gccaccgagc cgatcggcga caaggaccgc gcgcgcctgc ggctcgacgg caaggcgacg
    25081 cagctcgagt tccgcggcgc cgacggcaag gccctggcga cgctgctggt cggcaagaaa
    25141 tatttcaagc gcgagcccga ggacgcgggg aaggcggccg ccgacggccg cttcgtaatg
    25201 cgccccgagt cgcccaacac cgtcgtcatc gtcgccgacc cgctgcccct ggccacggcg
    25261 caaagcgcga gctgggtggc gaagaccggc ttcggcgccg agcgggtgcg cagcatggag
    25321 gtggatctcg ccggcgccgg caagtggcgc gtcgagcggg cgggcctgga gagcgactgg
    25381 aagctgatcc cgctctacgc gggcgaaaag ctcgacgtgc tgcgcgccaa ctcggcgacc
    25441 tactcgctca accgcgtcga gatcgtcgac gtcgcggcgc ccggcgcgcg caatgaggtc
    25501 acgggcctcg acaagccgac gaccgtggtc gcctcgacgc tcgacggcct cacctatacg
    25561 atcaagctcg gcaagctgcg cggcgacgag tacttcgtga cgctgtgggt ctcgggcgag
    25621 cccaagccca ccggcgccga cgccgccgag cgcgcgaaga gcatcgcggc gcgcctgccc
    25681 gccgagaagg cgctcgccgg ccacatcctg ctcatctcgc gctcgaagtt cgaggacgtt
    25741 ctcaagaaac gcgcagaact gctggagaag aaggaaaccg gcaagaagta gccgagccgc
    25801 ggcttcccga agtaggatag gcgcaggagg agatgcgatg ggcaagcatg ccatcaagca
    25861 cctgaggacc atcgcgctcg tcggccaggc gggaagcggc aagacctcgc tggccgaagc
    25921 gctgctggcg cgcgcggggg cggtacccgc cggcggcagc gtcgagcgcg gcagcacagt
    25981 gtgcgactac acgccgctgg aaaagcagct gcagcattcg ctcaagctcg ccgtcgccag
    26041 cttcgaggta cccaacggcg aggcgcccac cgtcatccat ctgctcgata cccccggcta
    26101 cgccgatttc ctcggccacg cgctgcccgc gctcgcggcg gtggagacgg cggcggtcgt
    26161 catcaacgcg cagaacggca tcgagatgat gaccgggcgc ttcctcagct gggcggccca
    26221 ccgcggcctg gaccggctga tcatcgtcaa caagatcgac gccgacaacg tggatctcga
    26281 gggcctgctc ggcgagatcc aggaggcttt cggcaaggag tgcctgccgc tcaacctgcc
    26341 ggcggcgaag gcgacgaagg tctccgactg cttcttcgcg ccctcgggcg aggcggattt
    26401 ctcctcggtg gaggccgcgc accgcaacct cgtcgaccag gtggtggagg tcgacgaaaa
    26461 gctgatggcc gagtacctgg agaagggcga gatcgaggcc gaggcgctgc acgagccgct
    26521 ggaagcggcg atgcgcgagg ggcacctgat cccggtggtg ttctgctcgg cgaagaccgg
    26581 cgccggcgtg caggagctcc tcgacgtgat cgtgaagctc ctgcccaacc cggccgaggg
    26641 caacccgccc gtgtaccacg gcgtgccggc cgacggcggc gcccccacgg agatccaggc
    26701 tacgccggac ccgtcgcagc acgcgctcgc gcacgtgttc aaggtcgaga tcgaccccta
    26761 catcgggcgg ctggccgtgt tccgcgtgca ccaggggcgc atcacgcccg gcatgcagct
    26821 gtatgtcggc gaaggcagga aacccattaa accggggcac ctttacctgc tgcgcggcaa
    26881 gacgcagacc gaggtgagcg aggcggtgcc gggcgacatc tgcgccatcg ccaaggtcga
    26941 cgagatccag ttcgaccaca tcctgcacga cgcggccgag gacgcgcaca tccacgccaa
    27001 gccgctcgag cttcccacct cggtgttcgg cctcgcgctg cagccgaaaa agcgtggcga
    27061 cgagcagaag gtgtccgacg tgctgcacaa gctggtggcc gaggatccgt gcctgaagat
    27121 cgagcacaac gcgcaggcca acgaaaccgt gatgcgcggc ctgggcgagc tgcacctcag
    27181 gaccacgctg gagaagatcg ccacacaaca caagctcgag ctcgacacgc gcccgccgtc
    27241 gattccgtac cgcgagacca tcgcggcgcg cgccgaaggg cacaaccggc acaagaagca
    27301 gaccggcggc gcgggccagt tcggcgaggt gttcctgaaa gtcgagccgc tgccgcgcgg
    27361 cgcgggcttc gagttcgtcg acagcgtgaa gggcggcgcg atccccaacc agttcatccc
    27421 gtcggtggag aaaggcgtgc gcgcggtgct ggagagcggc tacgtcgccg gctacccgat
    27481 ccaggacgtg cgcgtcatcg tctacgacgg caagagccac ccggtcgatt ccaaggacgt
    27541 ggcgttcatg tcggcgggcc gcaaggcctt cctcgatgcg ctcggcaagg cccgcccgat
    27601 cgtgctcgag ccgatcgtca acgtgcagat cgtcgccccg gagcagaaga tgggcgacat
    27661 cgccggcgag ctctcgggac accgcggcca gatcaagggc tcggacgcgc cgcgcccggg
    27721 caccgtggaa atcacggcgc aggcgccgct ctccgagatg gagcagttcc cggcgcgcct
    27781 gaagtcgatc actgccgggc atggctcgta ttcgctcgaa ttcagccact acgagcccgc
    27841 gccgccgcag ctgcagcaaa agctgatctc ggcgcatcgc ccggcggcga gcgccgacga
    27901 ttaaataggg acagacctca tttatccgcg ccgcttgcgc gccttgcgct tgcgcggcag
    27961 tggtgccggg ttggggatct cctcggccac gacgaaggtc ccgccctggt acagctccgc
    28021 gaccgggtca ccgggctttt cgcgtgccag cacttcggcc gcgaacgact ccgaatcgtc
    28081 ctgcggccgg tagccgagcc ggaaggcgtt cgcgttgtcg taccaggcgc gcttgttgcc
    28141 ggagatgccg tagacgatct cgaagcggat ttccggatgc tcgatgccga tcgtcaccag
    28201 ctgcgcgagg tcccgcggcg agatccagat cgagagccgc cgcttgtcga tcggcgccgg
    28261 gttgacgttg ccgatgcgga tctggaacac ctgcatgccg tacttgtcgg cgtagaggct
    28321 gcccagcgcc tcgccgaaca ccttggacac gccgtagcgg ctgtcgggtt tcggatagac
    28381 acggtggtcg atggtctggt cgcgccggta aaagcccacc gcatggttcg aggtaggaaa
    28441 gaggatgcgc ttcacgccgt tgcgtcgtgc ggcctcgaac acgttgtagc agccaatgat
    28501 gttggcgctg aggatccctt cccagggccc ttccaccgag tagccaccca ggtggatgat
    28561 cgcgtccacg cccttcgtaa tgcgcagcgc cgccttgaaa tccgagatgt cggcgcgcat
    28621 gacctgctgg cccttgaact tctccttcag ctcacgcagg tcggacgcgc ggatgcggta
    28681 cttgcccgcc agctcgcgcc gcaggtgggt gccgacgtcg ccggcggccc cggtgatgag
    28741 cacggtcttc atggttcctc cccgagccag tatagcgatt gccgccgccg cggcgctgtg
    28801 ctactaagca agtcccgtgc tccgcgcgct cgtccttgcc atcgccttcg ccgccgccgc
    28861 cggcgccccg ccggcgcggg ccgaggctac gccgcaagtc gtgatgtacg cgaccgcctg
    28921 gtgcccgtac tgcgcgaaag cgcgcgaata cttcgccgag tcgggcatcg cctatgtcga
    28981 gcacgacgtc gagaagtcgg cgcgcgccaa cgccgagttc aagcgcctgg gtggccgcgc
    29041 cgtgccgctc atcgtggtcg gacgcgagaa gatgagcggc ttcagcgagc agggtttcga
    29101 agacatgctc gcgcgcgcct cgcgctgacg atgacctggc aaccgagcca gcgcgttccc
    29161 gacgccgcgg tgcacgacgt ggacgcgtcc ttcgcgcgct accgcctgcc gctcgccaag
    29221 atcgatcgtc tcgccgccgg cctcacgcgc tgggccgagg ggccggtgta cttcggcgac
    29281 cagcgctgcc tcgtctggag cgacgtgccg ggcgactgca tgtggcgctg ggacgaagaa
    29341 acgggcgcgg tcggcgtctg gcgcaagcct tccggccacg ccaacggcaa cacgcgcgac
    29401 cgccaggggc gcctcgtcac ctgcgagcac agcggccggc gcgtctcgcg caccgagccc
    29461 gacggcaccg tgaccacgct cgccgaccgg ttcgacggca agcggctcaa ttccccgaac
    29521 gacgtcgtgt gcaagaccga cggctcgatc tggttcaccg acccgagctt cggcatcctc
    29581 ggctggtacg agggccgcaa ggcgcccgcc gagctgccga cgaacgtcta ccgcatcgat
    29641 ccctccggcg cgatgagcgt ggtcgccgag ggcatcaacc agcccaacgg cctcgcgttc
    29701 tcgcccgacg agtcgatcct ttacatcgtc gagtcgcgct cggtgccgag gaagatcctc
    29761 gcctgcgacg tggtgggcgg gaagaccctc tccaaccggc gcgtcctgat cgacgccggg
    29821 ccgaaaggca cgcccgacgg ctttcgcgtc gacgtggacg gcaacctctg gtgcggctgg
    29881 ggcatgggcg aagcgggcct ggacggcgtg cacgtgttca acccggacgg caagctgatc
    29941 ggccgcatcg acctcccgga gcgctgcgcc aacgtgtgct tcggcgggct gcaccggaac
    30001 cgcctgttca tggcggcgac cacgtcgctc tacgcgctct acgtcaacac gcagggcgtc
    30061 gcgaacggct agctgaactc gatcacgccc ttgcccgagg cctgcttgtc gaagagcttg
    30121 taggcctggt cggcctggtc gagcttccag cggtgggtga agatcctctc gagcgggatc
    30181 ttgttatcgg cgacaaagcg cgcgcactcg cccatgccca tcgcgctgaa ggtccaggag
    30241 ccgatgagcg tgagctgctt tcgcagcatg tcgcgcgaga catccagcgt cacatcgccg
    30301 ccttcgccca cgaagcacac cgtgccccag gtcttcgccg cgcgcaccgc ctgcagccgc
    30361 gccgcggcga cgcccgtgca atcgagcgcg aggtccacgc cttcgccgcg cgtcaggtcc
    30421 ttcaacgcct tctcgacgtc ggtcttggag gcgtccacca gcgcatcggc gccgtactgc
    30481 ttggcgagcg cgaggcgctc ggcgttcgct tccaccgcga tcacgcgcag gcccatcttc
    30541 gagccgagca gcgtggcgct gatccccacc ggcccctgcc cgaacaccgc gagcgtggcg
    30601 ccgccggcga cgtgcatgcg ccgcagcgcg ccgtaggcgg tgcccgtgcc gcaggccacc
    30661 gccgcgccct cggcgaacga caactcctcg ggcagcggca cgatcgtgcg taccggcacc
    30721 ttcaggtact cggcgtgccc gccgtgcgcg gtgacgccat acaccgtgat gcccgcgcgg
    30781 cagagctgcg accagcccaa gcggcagtgc gggcacacgc cgcagcccgc gtaatggtgg
    30841 tccatcacgc gcaggcccgt cggtgcttcc ttttccgaga cgcccggtcc gcgcgccacc
    30901 acctcgccgc agggctcgtg cccggcgatg aacggctcgg cgtcgccgcc caggccaagc
    30961 gccttttgcg cctcgcccgg cgccgagcgg tagaacttga ggtcgctgcc gcacatgccg
    31021 gaggcgcgca tgcgcagcac cacttcgccc ggaccgggcg tcgggtcggg aaagttgcgg
    31081 atttcgatct tgcgattgcc gaggaatact gcgccgcgca tgtcggatga ccctccccaa
    31141 tgttgccctg caatgtggcc gccatcatac cggacggttt ccttgcattg cttatcttaa
    31201 gctaggcaac tatcatcggg catgttccgc gaggacctgt cccgcaactt cggcttcctg
    31261 ctgaacgacg tggcgcggct gctgcgtacc gcctacgacc ggcgcgtgcg caagctcggg
    31321 ctgacgcggg cgcagtggtg ggtgctcact cacttgttcc gcagcaacgg cgtcagtcag
    31381 accgagctcg ccgagatcct ggagatcgag aagccgacac tcgggcggct gctcgaccgc
    31441 ctggaagcca agcgctgggt gcgccgcgag cacgatgcgc gcgaccggcg cgtctggcgc
    31501 gtgcac
//
LOCUS       DBNHKE010000234         6349 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_838265, whole genome shotgun sequence.
ACCESSION   DBNHKE010000234 DBNHKE010000000
VERSION     DBNHKE010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_838265"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2051
                     /gene="polA"
                     /locus_tag="AC16O9_16090"
     CDS             <1..2051
                     /gene="polA"
                     /locus_tag="AC16O9_16090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR6518177.1"
                     /translation="GEIGGAVGATLQRSLDWLPTGRSLLTVKRDVALPLKLEDLRAAP
                     DAEKQRALYERFGFKGWLAELGDGAAPATPRAEPGQAERRTYLTLADESALKDWLAQM
                     ERAELVGFDTETTGLEPMVAELVGMSFAFGAEAAYLPLAHQYPGAPEQLGVGRALELL
                     KPWLESTRHRKVGQNLKFDAHVLANHGVRLAGIAHDTLLESYVLEVHERHDLDSLAQR
                     HLGWKTIGYDEVTGKGAARIPFSAVSIERATEYAAEDADCALAVHAALYPRIAGEAKL
                     KRVYETIEMPVLPVLLGMERNGVLLDRDKLEAQSHELGKEMLELEQKAYGAAGQPFNL
                     NSPKQIQEILFERQQLPVKKKTPSGQPSTDEDVLAELAQDYPLPRLLLEYRAIAKLKS
                     TYTDKLPKMIHPQTGRVHTTYSQAVAVTGRLASNDPNLQNIPIRTPQGRRIREAFVAA
                     PGCRIVSADYSQIELRIMAHLSGDEGLRHAFAHGQDVHRATAAEVFGRTLEQVSDDER
                     RTAKVINFGLIYGMSAFGLAQNLGIERTTAQAYIDSYFSRYPGVKRYMDGTRDKARRE
                     GYVETVFGRRLWLPEIKSSNPQRRAGAERAAINAPMQGTAADLIKLAMIAVQGWLDAQ
                     RLGARLIMQVHDELVLEVPEAELARVQDGLRSRMQDVAALDVPLIVDMGVGANWDQAH
                     "
     gene            2120..3142
                     /gene="dctP"
                     /locus_tag="AC16O9_16095"
     CDS             2120..3142
                     /gene="dctP"
                     /locus_tag="AC16O9_16095"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR6518178.1"
                     /translation="MQGTRTCIAAALAGALAALPAPAAAQKVNWKFSSWGNPRAFTKG
                     IEVLAETVAKQSGGDFTITIGMGEQFSKARENLDGIKLGALDAAHFCNFYHPGKNPAF
                     MVFSLPFLPLGDWDVSLKVRTTLFKHPALVADMDNWNAVTYATTLLPQYEFLGKGKPP
                     VTLADWKGKRVRAGGGLGDAMEVLGAIKTTTAATEVYTSMQRGTMDAASFPYTYAQVA
                     YKIHEVSDWYTTNMSPGTSECPLVFNKSSWAKLPDQYRKMVTGALDAVNQAQIQAYVD
                     IDKKNLPMLAEKLKPVTYSKAQLEEFRRVAGKPVWDKWVSENKDKFDAQGVLDLVFKT
                     AKEAGG"
     gene            3232..3726
                     /locus_tag="AC16O9_16100"
     CDS             3232..3726
                     /locus_tag="AC16O9_16100"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6518179.1"
                     /translation="MFLGIAQVLGRQLFNLPISGYIDFVELSMATFAFLGVAYCQRHG
                     GHVRMEMVLKFARGRWQWALEVFGTLVALFIIAVLIWYGYGHFLRAWTLGDSTIDAEL
                     PVWPSKLIVPIAFALLWLRLLVQLVGYARLVLRPDAQPVAVPVILSAEELARKEIEES
                     AEKR"
     gene            3728..5029
                     /locus_tag="AC16O9_16105"
     CDS             3728..5029
                     /locus_tag="AC16O9_16105"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6518180.1"
                     /translation="MDSFTIGIVGTIAMVVLVFTGVRVFYAAAAVGLVGLVAMIGWDA
                     GAGMAGTIPHSKSVTYTLSVLPMFILIGYLAFHAGLTEALFDAARKWLGWVPGGLAVA
                     TIMAATGFAAVSGASTATAAVFARVAVPDMLKAGYDKRLAAGVVAAGGTLATLIPPSA
                     LLVIYAIIVEESVGALLVAGFLPGLVSALIYVALIVIMAKLNPKLGAAIRGYSWRERW
                     KSVPGTAPIIAVVVIIFSAMYFGWATPTEAGALGAFVVFVLALVHGMRWRGLKDALLE
                     TAKLTVMIFSIIWGVLLFVRFLGFAGLPDAFAKWVVALDVPPLAILICILLFYVVLGM
                     FMDAIGMLLLTLPVVYPAVVALGYDPIWFGIIVVKMAEICLITPPIGLNCFVVNGVRP
                     DIPLSSVFRGIFPFFIADVVTVGVFIAFPEVILWLPRTMNM"
     gene            complement(5095..5769)
                     /locus_tag="AC16O9_16110"
     CDS             complement(5095..5769)
                     /locus_tag="AC16O9_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HIR6518181.1"
                     /translation="MRVAPLIVGLVIAAPAWAQLESITNREAINALKGALDRGARAAV
                     AQLGREDGFLGDARVRIALPEPLRRAEKNMRRFGMSRYADELILTLNRAAEAAVPEAK
                     QLFVDALRQMSVQDAKGILTGGDTAGTAYFRRTTEGELGRRFLPIVQRATARVGLAQK
                     YNQYAAHGVRVGLVGAEDASLDDYVTRKALDGLFFMVAEEEKKIRKDPVGTASSLLRK
                     VFGALK"
     gene            5782..6222
                     /locus_tag="AC16O9_16115"
     CDS             5782..6222
                     /locus_tag="AC16O9_16115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR6518182.1"
                     /translation="MKTQIKLIAFVAVAAIVAGAAFGVLRRAQTLQARFVALRGANFS
                     TADLRRRTVLVNFWATSCTTCVAEMPKLVAAWNKYAPRGYETVAVAMSYDHPNAVAQF
                     AQARSPRPRDAAQHILSVTARAWGSARQPPKPVRKRTSGVLGVL"
BASE COUNT          996 a         2179 c         2130 g         1044 t
ORIGIN      
        1 ccggcgagat cggcggcgcg gtgggcgcga ccctgcagcg ctcgctcgac tggctgccca
       61 cgggccgcag cctgctcacc gtgaagcgcg acgtcgcgct gccgctcaag ctcgaggatc
      121 tgcgcgccgc gcccgacgcc gaaaagcagc gcgcgctcta cgagcgcttc ggcttcaagg
      181 gctggctcgc cgaactcggc gatggcgcgg cgccggcgac cccccgcgcg gagcccgggc
      241 aggccgagcg ccggacgtat ctcacgctgg ccgacgaatc cgcgctcaag gactggctgg
      301 cgcagatgga gcgcgccgag ctggtcggct tcgacaccga gaccaccggc ctcgagccga
      361 tggtggcgga gctcgtcggc atgtcgttcg cgttcggcgc cgaggccgcg tacctgccgc
      421 tcgcgcacca atatcccggc gcgccggagc agctcggcgt cgggcgtgcg ctcgagctgc
      481 tcaagccctg gctggaaagc acacgccacc gcaaggtcgg ccagaacctg aagttcgacg
      541 cgcacgtgct cgccaaccac ggcgtgcggc tggccggcat cgcgcacgac acgctgctcg
      601 agtcgtacgt gctcgaggtg catgagcgcc acgatctgga ttcgctcgcg cagcgccacc
      661 tgggctggaa gaccatcggc tacgacgagg tcaccggcaa gggcgcggca cgcatcccgt
      721 tctccgccgt cagcatcgag cgcgccaccg agtacgccgc cgaggacgcc gactgcgcgc
      781 tcgcggtgca cgcggcgctc tacccgagga tcgctggcga ggcgaagctc aagcgcgtct
      841 acgagacgat cgagatgccg gtgctgccgg tgctgctcgg gatggaacgc aacggcgtgc
      901 tgctcgatcg cgacaagctc gaggcgcaaa gccacgagct cggcaaggag atgctcgagc
      961 tggagcaaaa ggcctacggc gcggccggcc agcccttcaa cctgaattcg cccaagcaga
     1021 tccaggagat cctgttcgag cgccagcagc tgccggtgaa gaaaaagacg ccctcgggcc
     1081 agccctcgac cgacgaggac gtgctcgccg agctggcgca ggactacccg ctgccccggc
     1141 tgctgctcga gtaccgcgcg atcgcgaagc tgaagtccac ctacaccgac aagctgccca
     1201 agatgatcca tccgcagacc gggcgcgtgc acaccaccta ctcgcaggcg gtggcggtca
     1261 ccgggcggct cgcctccaac gatcccaacc tgcagaacat cccgatccgc acgccgcagg
     1321 gccggcgcat ccgcgaggcg ttcgtcgccg ccccgggctg ccgcattgtt tccgcggact
     1381 actcgcagat cgagctgcgc atcatggcgc acctctcggg cgacgagggg ctgcgccacg
     1441 ccttcgcgca cggccaggac gtgcaccgcg ccaccgccgc cgaggtgttc gggcgcacgc
     1501 tggagcaggt gagcgacgac gagcggcgca ccgccaaggt gatcaacttc ggcctgatct
     1561 acggcatgag cgcgttcggc ctggcgcaga acctcggcat cgagcgcacc acggcgcagg
     1621 cctacatcga ctcgtacttc tcgcgctacc cgggcgtcaa gcgctacatg gacggcacgc
     1681 gcgacaaggc gcgccgcgag ggctacgtgg aaacggtatt cgggcggcgc ctgtggctgc
     1741 cggagatcaa gtcgtcgaac ccgcagcggc gcgccggcgc cgagcgcgcc gcgatcaacg
     1801 cgccgatgca gggcacggcc gcggacctga tcaagctcgc catgatcgcg gtgcagggct
     1861 ggctcgacgc gcagcggctc ggcgcccggc tcatcatgca ggtgcacgac gagctggtgc
     1921 tcgaggtgcc ggaagcggag ctcgcgcgcg tgcaggacgg actgcgcagc cgcatgcagg
     1981 acgtggcggc gctcgacgtg ccgctgatcg tcgacatggg cgtcggcgcc aactgggacc
     2041 aggcgcactg acccgggttg tcggccgttc tggccctgtg ctaccgtcct gacgcatcca
     2101 tcgaggagga ggaaagacca tgcaaggcac ccgcacgtgc attgccgccg cgcttgccgg
     2161 cgcgctggcc gcgctcccgg ctcccgccgc ggcgcagaag gtgaactgga agttctccag
     2221 ctggggcaac ccgcgcgcct tcaccaaggg catcgaggtg ctcgccgaga ccgtggccaa
     2281 gcagtccggc ggcgatttca ccatcaccat cggtatgggc gagcagttct cgaaggcccg
     2341 agagaacctc gacggcatca agctcggcgc cctggacgcc gcgcatttct gcaacttcta
     2401 ccacccggga aagaaccccg cgttcatggt gttctcgctg cccttcctgc cgctcggcga
     2461 ctgggacgtg tcgctcaagg tgcgcacgac gctcttcaag caccccgcgc ttgtcgcgga
     2521 catggacaac tggaacgcgg tgacctacgc cacgaccctg ttgccgcagt acgaattcct
     2581 cggcaagggc aagccccctg tgacactcgc ggactggaag ggcaagcgcg tgcgcgcagg
     2641 cggcgggttg ggtgacgcga tggaggtgct cggcgccatc aagaccacta ccgcggctac
     2701 cgaggtctac acctcgatgc agcgcggcac catggacgcg gcctcattcc cgtacaccta
     2761 tgcgcaggtc gcctacaaga tccacgaggt ctcggactgg tacacgacca acatgtcgcc
     2821 cggcacctcg gagtgcccgc tcgtcttcaa caagagctcc tgggcgaagc ttcccgacca
     2881 gtacaggaag atggtcaccg gggccctgga tgccgtgaac caggcgcaga tccaggccta
     2941 cgtggacatc gacaagaaga acctgccgat gctcgctgaa aagctcaagc ccgtaaccta
     3001 cagcaaggcg cagctggagg agttccggcg cgtcgcgggc aagcccgtct gggacaagtg
     3061 ggtgagcgag aacaaggaca agttcgacgc gcagggcgtg ctcgacctcg tcttcaagac
     3121 cgccaaagag gccggcggct gagcgccccc gcgctcgagc gcttcgaccg taggctcggc
     3181 cgcgtcgagg agcttttcaa cctcgtcgcg gccgcctgta ttttcgggct gatgttcctc
     3241 ggcatcgcgc aggtgctggg gcggcaactg ttcaacctgc cgatctcggg ctacatcgac
     3301 ttcgtcgagc tgtcgatggc caccttcgcg ttcctcgggg tggcctactg ccagcgccac
     3361 ggcgggcatg tgcggatgga gatggtgctg aagttcgccc ggggccgctg gcaatgggcg
     3421 ctcgaagtgt tcggcacgct cgtggcgctg ttcatcatcg cggtgctgat ctggtacggc
     3481 tacgggcatt tcctgcgcgc ctggacgctc ggcgacagca cgatcgacgc ggagctgccg
     3541 gtgtggccct cgaagctcat cgtgccgatc gccttcgcgc tgctgtggct gcggctgctg
     3601 gtgcagctcg tcggctacgc gcggctcgtg ctgcggcccg acgcgcagcc cgtggcggtg
     3661 cccgtgatcc tcagcgccga ggagctggcg cgaaaggaaa tcgaggaaag cgcggagaag
     3721 cgctagcatg gattcgttca ccatcggcat cgtcggcacc atcgccatgg tggtcctcgt
     3781 cttcaccggg gtacgcgtgt tctacgcggc agcggcggtc gggctggtcg gcctcgtcgc
     3841 gatgatcggc tgggacgcgg gcgcgggcat ggcgggcacc atcccgcact ccaagtccgt
     3901 cacttacacg ctctcggtgc taccgatgtt catcctcatc ggctacctcg cgttccacgc
     3961 cggcctgacg gaagcgctgt tcgacgcggc gcgcaagtgg ctcggctggg tgccgggcgg
     4021 gctcgcggtg gcgacgatca tggccgccac gggtttcgcc gcggtctcgg gcgcgagcac
     4081 cgccaccgcg gcggtattcg cgcgcgtcgc ggtgcccgac atgctgaagg cgggctacga
     4141 caagcgcctc gcggcgggcg tcgtcgccgc gggcggcacg cttgcgacgc tcatcccgcc
     4201 gagcgcgctg ctcgtcatct acgccatcat cgtcgaggag tcggtgggcg cgctgctggt
     4261 ggcggggttc ctgccgggcc tggtgtccgc gctgatctac gtggcgctca tcgtcatcat
     4321 ggccaagctc aacccgaagc tcggcgccgc gatccgcggc tacagctggc gtgagcgctg
     4381 gaagtccgtg cccggcacgg cgccgatcat cgccgtcgtg gtgatcatct tctccgccat
     4441 gtacttcggc tgggcgacgc cgaccgaggc cggcgcgctc ggcgcgtttg tcgtcttcgt
     4501 gcttgcactc gtgcacggca tgcgctggcg cgggttgaag gacgcgctgc tggagaccgc
     4561 gaagctcacg gtgatgatct tctccatcat ctggggcgtg ctgctcttcg tccgcttcct
     4621 cggctttgcg ggactgccgg atgcgttcgc caagtgggtg gtggcgctcg acgtgccgcc
     4681 gctcgcgatc ctcatctgca ttctgctgtt ctacgtggtg ctgggcatgt tcatggacgc
     4741 catcggcatg ctgctgctta cgctgccggt ggtgtacccg gccgtggtcg cgctcggcta
     4801 cgacccgatc tggttcggca tcatcgtcgt caagatggcg gagatctgcc tcatcacgcc
     4861 gcccattggc ctgaactgct tcgtcgtgaa tggcgtgcgt cccgacatcc cgctgtccag
     4921 cgtgttccgg ggcatctttc cattctttat cgccgacgtg gtcaccgtcg gcgtgttcat
     4981 cgccttcccg gaggtcatcc tctggctgcc gcgtaccatg aacatgtaaa tagggacaga
     5041 cctcattttt ttcgttcggg acccggccgc gaatgagaaa aatgaggtct gtccctattt
     5101 aagggcgccg aagaccttgc gcagcaggct ggaggcggtg cccacggggt ccttgcggat
     5161 cttcttctct tcctcggcca ccatgaagaa cagcccgtcg agcgccttgc gcgtcacgta
     5221 gtcgtcgagg ctcgcgtcct cggcgccgac gaggccgacg cgtacgccgt gcgctgcgta
     5281 ctggttgtat ttctgcgcca agccgacgcg cgccgtagcg cgctgcacga tcggcaggaa
     5341 tcgccgcccc aactcgccct cggtcgtccg gcggaaatac gccgtccccg ccgtgtcgcc
     5401 gcccgtgagg atgcccttcg cgtcctgcac gctcatctgc cgcagcgcgt ccacgaacag
     5461 ttgcttcgcc tcgggcaccg cggcctcggc ggcgcggttc agcgtcagga tcagctcgtc
     5521 ggcgtagcgc gacatgccga agcggcgcat gttcttctcg gcgcgccgca acggctcggg
     5581 cagcgcgatc ctgacccgcg cgtcgccgag aaagccgtct tcgcgcccca gttgcgccac
     5641 ggctgcccgc gcaccgcgat caagggcgcc cttcagggcg ttgatcgcct cgcggttggt
     5701 gatgctttcg agctgcgccc aggcgggcgc cgcgatgaca aggccgacga ttagcggggc
     5761 aacgcgcacg ctgatctcca gatgaagacg cagatcaagc ttatcgcctt cgttgccgtg
     5821 gcggcaatcg tcgccggcgc cgcgttcggc gtcctgcggc gtgcgcaaac gctgcaggcg
     5881 cgctttgtcg cgctgcgcgg agccaatttc agcaccgccg atctgcgcag acgcacggta
     5941 ctggtgaatt tctgggccac ctcctgcacc acctgcgtgg ccgagatgcc gaagctggtg
     6001 gcggcgtgga acaagtacgc cccgcgcggc tacgagacgg tggcagtggc gatgagctac
     6061 gaccatccca acgccgtggc gcaattcgcg caggcgcgct cgccgcgccc gcgtgacgcc
     6121 gcgcagcaca tactgagcgt caccgcccgc gcctggggca gcgcacggca gcctccgaaa
     6181 ccggtgcgta aacgcacttc gggggttttg ggggtcctct aaagtggggt catggcttcg
     6241 gcggtgccgg agcggacgat taaccacttg aaaggagcct caccatgaaa cgccaagttc
     6301 tcgcagtcgc cctcggcacc gtgtttgcgc tgccggccct cgccaacaa
//
LOCUS       DBNHKE010000235         8133 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1367763, whole genome shotgun sequence.
ACCESSION   DBNHKE010000235 DBNHKE010000000
VERSION     DBNHKE010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1367763"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..739)
                     /locus_tag="AC16O9_16120"
     CDS             complement(119..739)
                     /locus_tag="AC16O9_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518183.1"
                     /translation="MSDAKLARQPALAARLRRAAERLGAPGLVGAAILATCLGYYGGS
                     LQPLAQKIEALRALQATGAAPRARAPQQDLAETLRQFSAAFPAEGEVAGLLKQLYGLG
                     ERAGLRLTQGEYRYVAADALGMVQYRVTLPVRGRYPEIRQFIGAALAEVPWAAVTQVH
                     LQRERVGDGRIDARVELTLHLRAGGAGVAGATVAAEPFAVAREGAR"
     gene            complement(736..1272)
                     /locus_tag="AC16O9_16125"
     CDS             complement(736..1272)
                     /locus_tag="AC16O9_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518184.1"
                     /translation="MKRVPALQLDHFARGTRGTGAGWMLLAAALVAAVALGAWQSGLQ
                     RELDAREAQASSAERDYRVRTAASAVDPRRLEDAQRRARRIALELRLPWNELFRAVEE
                     AADPAVALLAVEPDVSRGALRVTGEAKNKDAMLRYVRRLGAHKPIVRAMLESHVERPA
                     AARAPVQFTLVAYWEGGP"
     gene            complement(1269..2069)
                     /locus_tag="AC16O9_16130"
     CDS             complement(1269..2069)
                     /locus_tag="AC16O9_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518185.1"
                     /translation="MSPRWRSETAILLTPTRVALRQSVRGLRPAPARVAALALEGAER
                     HWQAPLAALDGLLAAARARGGEASVVLSSHFVRYCLVPPSELLATREDEVRFAQQNFV
                     RIHGAAAEGWSVRVGPGLAGGAAVASGVERALVDDLRALLARHGLRPRALQPALMALF
                     NRARGELPAAACRVVALEPGIALSALLAPGWRELRSQGVPGPLGEALEPLLARERALE
                     DEDAAGIQTCVLPLLPFAAAAALGEGVRVLEPWWNDAPAHAAGEREAA"
     gene            complement(2050..3732)
                     /locus_tag="AC16O9_16135"
     CDS             complement(2050..3732)
                     /locus_tag="AC16O9_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6518186.1"
                     /translation="MNAPAPAFVLDAAALRRAREDARRDGASALALLEERAGLAPREF
                     VAQLGALLRYPALGNAELSALAPDYAALPFAEASRRGVIFARDAAGTLLAVCADPLNA
                     ELREWAETRVRHDFGWAIAHRDDLAVYLARQEDALRAIDAALVPAASANDAAALEDLS
                     LASISEDTNPTVRLVNSTLYDALRTGASDIHLESRAGGLEIKYRIDGVLSAVGQVAGR
                     EQAQQVISRVKVMSELDISETRVPQDGRLKVGVRGREVDLRVSIMPSIFGEDAVLRIL
                     DKQSLADRVTGLTLTQLGFDAATVAGLRRLSREPYGMLLVTGPTGSGKTTTLYAAISE
                     INHGRDKIVTIEDPVEYQLSGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDP
                     ETAQIGVQAALTGHLVLTTVHANNVFDVIGRFTHMQVDPYNLVSALNGVLAQRLVRLN
                     CPECSGPVRVEAAELAESGIARREAEGWRFRAGRGCGVCRGTGFKGRRALGELLRLTP
                     EIAELIVSRAPQRALRAAAEAAGTRFLRQAALDLVKSGETTLDEVNRVTAVA"
     gene            complement(3729..4916)
                     /locus_tag="AC16O9_16140"
     CDS             complement(3729..4916)
                     /locus_tag="AC16O9_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6518187.1"
                     /translation="MRYRVLAISARSDPVELTLEAADAEHARREAAERGLHVVGVRAA
                     RPWALRRPGRARFPLLAFSQELCALLAAGIGLVESLQTLADKEERPAVRVVLEDVIRV
                     LSAGAPLSEALAAHPAAFAPLFVATVKAAEKTGDLPEALARFVAYRAQIDQVRKRAIG
                     ALIYPALLLGVGALVTLFLLAYVVPRFASLYQDAGRDLPFLSELLLAWGRFFEAHWAL
                     VLAGTAAFAVAAAQALTRPALAARLAEAPWKVPALGERLRVYQLARLYRTLGMLLESG
                     LPIVTALRQSSAMLAPQLQPRLERAVTALGGGRLISSALDESGLTTPVALRMLRVGER
                     SGRMSELMGRIATFHEDEMARSVEWFTRLFEPLLMLAIGALIGLIVLLMYVPVFELAG
                     SLQ"
     gene            complement(4921..5355)
                     /gene="gspG"
                     /locus_tag="AC16O9_16145"
     CDS             complement(4921..5355)
                     /gene="gspG"
                     /locus_tag="AC16O9_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR6518188.1"
                     /translation="MQLERLFPRRAASAGFTLLELLVVMVIIGLLAGYVAPRYFGQIG
                     KSEVKVARAQIDALEKAIDQYRLDTGRFPPQEGGLVALVAKPDNEPKWAGPYLRRDVP
                     LDPWGRPYVYRVPGQKSDYDLLSYGKDGRQGGSGEAEDIQNR"
     gene            5831..6034
                     /locus_tag="AC16O9_16150"
     CDS             5831..6034
                     /locus_tag="AC16O9_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6518189.1"
                     /translation="MTIGTVKFFNAAKGFGFIAPESGEKDVFVHISAVEKSGMGTLAE
                     GQRVSFEIVTERGKLAASNLQAA"
     gene            6110..6766
                     /locus_tag="AC16O9_16155"
     CDS             6110..6766
                     /locus_tag="AC16O9_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HIR6518190.1"
                     /translation="MSATSLPLLSSVEARVLGVLVEKQRAVPDTYPLTLNALVSGCNQ
                     KSSRDPVMDLDGAQAQEALDSLRRLTLVIESSGGRVPRYEHNMERVLQIPTTSAALLA
                     VLMLRGPQTAGELRINCDRLHKFADISAVEGFLHELAERPAGALVAQLPRQPGSRENR
                     WAHLLCGAPAAEPHAAPAAAPRAEAGGELASLRGRVEALEASVAELRALVAALQSARD
                     "
     gene            complement(6831..7898)
                     /locus_tag="AC16O9_16160"
     CDS             complement(6831..7898)
                     /locus_tag="AC16O9_16160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6518191.1"
                     /translation="MGGRLVVYGIGAIGGQVAGRLHGAGVALTAVEPWDAQREAVVRG
                     GLRIAGAEGGVERYPLPVIAPHELEGQVDLCLLAVKSYDTEAALRRVLPHLAPDALVV
                     SLQNSINEEQIAPRVGAGRVLGGVVLVNAVLLEPGLITVTASVSRAAAAVHLPGVYVG
                     EYLRPAGDNARRVARLLEAVWPAEPIDDLLRERWTKMVTNSALNPVSGLGGLGSAAML
                     ASAGARRAIAGIAAEVLRVAAAEGHRVARVMGDFSDEEVYAGALGASTAFDRGLAARA
                     ARVSADAATSLLQDVLRGRRTEVDYFSGLAARKGRAHGIATPWCDAATALVHDIEQGR
                     RAPSAANVEELARRALGAGAR"
     gene            complement(7900..>8133)
                     /locus_tag="AC16O9_16165"
     CDS             complement(7900..>8133)
                     /locus_tag="AC16O9_16165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6518192.1"
                     /translation="GLYGKAVVTDLAPARSDLVAQGLGAHGALVERPEQLAPALERAL
                     AAGKPALLNIAVQSQISMRAQGIVDSRKQGGAF"
BASE COUNT         1101 a         3081 c         2946 g         1005 t
ORIGIN      
        1 gcgaccggcg ctgcggcccg cggccgcgcc gcgtcggccg cgccggccgc gcggtaatcg
       61 tgcagcccga gcgccgcagc gagcgcggcg acccccgcgg cggcgagcgc gcggcggttc
      121 atcgcgcgcc ctcccgggcg accgcgaagg gctcggccgc aacggtggcg cccgccaccc
      181 ccgcgccccc ggcgcgcagg tgcagcgtca gctcgacgcg cgcgtcgatg cggccgtcgc
      241 ccacgcgctc gcgctgcagg tggacctgcg tgaccgcggc ccagggcact tcggcgagcg
      301 ccgcgccgat gaactgccgg atctccggat agcggccgcg caccggcagc gtgacgcggt
      361 actgcaccat gccgagcgcg tcggcggcga cgtagcggta ctcgccctgc gtgagccgca
      421 ggccggcgcg ctcccccagg ccgtagagct gcttgagcag gcccgcgact tcgccctcgg
      481 ccgggaacgc cgcggaaaat tgccgcagcg tctcggcgag gtcctgctgc ggcgcgcgcg
      541 cgcggggcgc ggcgccggtg gcttgcagcg cgcgcagcgc ttcgatcttc tgcgcgagcg
      601 gctgcagcga cccgccgtag tagcccaggc aggtggcgag gatcgccgcg ccgacgagcc
      661 ccggcgcgcc gaggcgctcg gcggcccggc gcaggcgcgc agcgagcgcc ggctggcgcg
      721 cgagcttcgc gtcgctcacg gcccgccctc ccagtaggcg accagcgtga actgcaccgg
      781 cgcgcgcgcc gcggcgggcc gctcgacgtg cgactcgagc atcgcgcgca cgatcggctt
      841 gtgcgcgccc aggcgccgca cgtagcgcag catggcatcc ttgttcttcg cctcgccggt
      901 cacgcgcagc gcgccgcggc tcacgtccgg ctccaccgcg agcagcgcca cggccgggtc
      961 ggcggcctcc tccaccgcgc ggaacagctc gttccacggc agccgcagct cgagcgcgat
     1021 gcgccgcgcc cgccgctgcg cgtcctcgag ccggcgcggg tccaccgcgc tcgccgccgt
     1081 gcgcacccgg tagtcgcgct cggcgctcga ggcttgcgcc tcgcgcgcgt ccagctcgcg
     1141 ctgcagcccg ctttgccagg cgccgagcgc cactgcggcg acgagcgccg cggcgagcag
     1201 catccagccc gcgccggtgc cgcgcgtgcc gcgcgcgaag tgatcgagtt gcagtgccgg
     1261 cacgcgcttc acgcggcctc ccgctcgccg gcggcgtgcg ccggggcgtc gttccaccag
     1321 ggctcgagca cgcgcacgcc ctcgccgagc gcggccgcgg cggcgaaggg cagcagcggc
     1381 agcacgcagg tctggatgcc ggccgcgtcc tcgtcctcga gcgcgcgctc gcgcgcgagc
     1441 agcggctcga gcgcctcgcc cagcggtccg ggcacgccct ggctgcgcag ctcgcgccag
     1501 cccggggcga gcagcgcgct cagcgcgatg cccggctcga gcgcgacgac gcgacacgcc
     1561 gccgccggca gctcgccgcg ggcgcggtta aacagcgcca tcagcgccgg ctgcagcgcg
     1621 cgcgggcgca ggccgtggcg cgcgagcagc gcgcgcagat cgtccaccag cgcccgctcc
     1681 acgccgctcg cgaccgcggc gccgcccgcg aggcccggac cgacgcgcac gctccagccc
     1741 tcggccgccg cgccgtggat gcgcacgaag ttctgctgcg cgaagcgcac ctcgtcctcg
     1801 cgcgtcgcga gcagctcgct cggcggcacc aggcagtagc gcacgaagtg gctcgagagg
     1861 acgacgctcg cctcgccgcc gcgcgcgcgg gccgcggcaa gcagcccgtc gagcgccgcg
     1921 agcggcgcct gccagtgccg ctcggcgccc tcgagcgcga gcgccgccac gcgcgcgggt
     1981 gcggggcgca ggccgcgcac gctctggcgc agcgcgacgc gcgtcggggt gaggaggatc
     2041 gcggtctcgc tacgccaccg cggtgacacg gttcacctcg tccaaagtgg tttcgccgct
     2101 cttcaccagg tcgagcgccg cctggcgcag gaaccgggtg ccggccgcct cggcggccgc
     2161 gcgcaaggcg cgctgcgggg cgcgcgacac gatcagctcg gcgatctcgg gcgtcaggcg
     2221 cagcagctcg ccgagtgcgc gccggccctt gaagcccgtg ccgcggcaca cgccgcagcc
     2281 gcggcccgcg cgaaagcgcc agccctcggc ctcgcgccgc gcgatgcccg attcggcgag
     2341 ctcggcggcc tccacgcgca cgggcccgga gcactccggg cagttcaggc gcaccaggcg
     2401 ctgcgcgagc accccgttca gcgcggagac caggttgtag gggtccacct gcatgtgcgt
     2461 gaagcgcccg atgacgtcga aaacgttgtt cgcgtgcacg gtggtgagca cgaggtggcc
     2521 ggtgagcgcc gcctgcacgc cgatctgcgc ggtctcgggg tcgcggatct cgccgaccat
     2581 gatcttgtcg gggtcgtggc gcaggatgga gcgcaggccg cgcgcgaagg tgaggccctt
     2641 tttctcgttc accgggatct gcagcacgcc ggacagctgg tactcgaccg ggtcctcgat
     2701 ggtgacgatc ttgtcgcgcc cgtggttgat ctccgagatc gcggcgtaca gcgtcgtcgt
     2761 cttgccgctg ccggtcgggc ccgtgacgag cagcatcccg tagggctcgc gcgacagccg
     2821 ccgcaggccc gccacggtgg ccgcgtcgaa gcccagctgc gtgagcgtga ggcccgtcac
     2881 gcggtcggcg agcgactgct tgtccaggat ccgcagcacc gcgtcctcgc cgaagatgct
     2941 cggcatgatg gacacgcgca ggtccacctc gcggccgcgc acgccgacct tgaggcgccc
     3001 gtcctgcggc acgcgcgtct cggagatgtc gagctcggac atcaccttca cgcgcgagat
     3061 gacctgctgc gcctgctcgc gcccggcgac ctggccgacc gccgagagca cgccgtcgat
     3121 gcggtacttg atctccaggc ccccggcgcg cgactccagg tggatgtcgc tcgcgccggt
     3181 gcgcagcgcg tcgtacagcg tcgagttgac caggcgcacc gtcgggttgg tgtcctcgct
     3241 gatcgaggcg agcgacaggt cctcgagcgc ggcggcgtcg ttggcgctcg ccgcgggcac
     3301 cagcgcggcg tcgatcgcgc gcagcgcgtc ctcctggcgc gcgaggtaca ccgcgaggtc
     3361 gtcgcggtgc gcgatcgccc agccgaagtc gtggcgcacg cgcgtctccg cccactcccg
     3421 cagctccgcg ttcaggggat cggcgcatac cgcgagcagc gtccccgccg cgtcgcgcgc
     3481 gaagatcacg ccgcggcgcg aggcctcggc gaagggcagc gccgcgtagt cgggcgcgag
     3541 cgcgctcagc tcggcgttgc cgagcgcggg atagcgcagc agcgcgccga gctgcgcgac
     3601 gaactcgcgc ggcgcgaggc cggcgcgctc ttccagcagc gcgagcgcgc tcgcgccgtc
     3661 gcggcgcgcg tcctcgcgcg cgcggcgcag cgccgccgcg tcgagcacga aggcgggcgc
     3721 gggggcgttc actgcaggct cccggcgagc tcgaacaccg gcacgtacat gagcagcacg
     3781 atcaggccga tcagcgcgcc gatggcgagc atcagcagcg gctcgaacag ccgcgtgaac
     3841 cactccaccg agcgcgccat ctcgtcctcg tggaaagtcg cgatccggcc catcagctcg
     3901 ctcatgcggc cggagcgctc gcccacgcgc agcatgcgca gcgccacggg cgtggtgagg
     3961 ccgctctcgt cgagcgccga ggagatgagc cgtccgccgc cgagcgcggt caccgcgcgc
     4021 tccaggcgcg gctgcagctg cggcgcgagc atggcgctcg actggcgcag cgccgtgacg
     4081 atcggcaggc cgctctccag gagcatcccg agcgtgcggt acaggcgcgc gagctggtac
     4141 acgcgcagcc gctcgccgag cgccggcacc ttccagggcg cctccgcgag gcgcgcggcc
     4201 agcgcggggc gcgtgagcgc ctgcgcggcc gcgacggcga acgccgcggt gccggcgagc
     4261 accagggccc agtgggcctc gaagaagcgg ccccaggcaa gcagcagctc ggaaaggaac
     4321 ggcaggtcgc gccccgcgtc ctggtacagg ctcgcgaagc gcggcaccac gtaggcgagc
     4381 aggaacaggg tgaccagcgc gcccacgccg aggagcagcg cgggatagat caaggcgccg
     4441 atcgcgcgct ttcgcacctg gtcgatctgc gcgcggtagg ccacgaagcg cgcgagcgcc
     4501 tcgggcaggt cgccggtctt ctcggccgcc ttgaccgtgg cgacgaacag cggcgcaaag
     4561 gccgccgggt gcgcggcaag cgcctcggag agcggcgcgc ccgccgagag cacgcggatc
     4621 acgtcctcga gcaccacgcg caccgccggg cgctcctcct tgtcggcgag cgtctgcagc
     4681 gattccacca gcccgatccc cgcggcgagc agcgcgcaca gctcctggct gaacgcgagc
     4741 agcgggaagc gcgcgcgccc cgggcgccgc agcgcccagg gccgcgcggc gcgcacgccc
     4801 accacgtgca ggccgcgctc ggcggcctcg cggcgggcat gttcggcgtc ggccgcctcg
     4861 agcgtcagct cgaccgggtc ggagcgcgcg gagatggcga gcacgcgata gcgcatgggc
     4921 ctagcgattc tggatgtcct cggcttcgcc gctgccgccc tggcgcccgt ccttgccgta
     4981 gctgagcagg tcgtagtcgc tcttttgtcc gggcacgcgg tagacgtagg gccggcccca
     5041 gggatcgagc ggcacgtcgc ggcgcaggta cgggcccgcc cacttcggct cgttgtcggg
     5101 cttggcgacg agcgcgacga gcccgccctc ctgcggcggg aagcgcccgg tatccaggcg
     5161 gtactggtcg atcgccttct cgagcgcgtc gatctgcgcg cgcgcgacct tgacctcgga
     5221 cttgccgatc tggccgaagt agcgcggcgc gacgtagccg gcgagcaggc cgatgatcac
     5281 catcacgacg agcagctcga gcagcgtgaa gcctgcgctc gccgcgcgcc ggggaaacaa
     5341 gcgctccagt tgcatgtcca cctccaggct cgcggacttc accatggccc gcggatcggg
     5401 cacaggcgga ccccctatag ccaggcctgg ggcgtacgcg cgccccggca acccacctag
     5461 gggttcgagt ctagtgtatg caccccctgc ctccggtgcg gtcaaatgtc acactttgac
     5521 gttcgccgca ctgcatgatc ggggcggaat gtccggaatc gtcatagggg aaacgccggg
     5581 cgtgttgcac ggcacaagcc ggagcggaat tcctctcctg cgccgggtcg gcttgcccat
     5641 gccgccgccg gcgccaaggc gcggtttgcg ccggaaccca atcgggcgca cactgcgccc
     5701 gcgatctacg tcttggcccg tctttcatcc acgcccggtt cccgggcgcc caatgcaaat
     5761 accccactac gactaggttg ttcgttgccg cgcccgattg tgcgcggcgc cttcgtaatg
     5821 gagaacccgc atgacaatag gaaccgtgaa attcttcaat gccgccaagg gattcggatt
     5881 catcgcaccg gaaagcggcg aaaaggacgt gttcgtgcac atcagcgccg tggagaaatc
     5941 gggcatgggc acgctggccg aaggccagcg cgtcagcttc gagatcgtca cggagcgcgg
     6001 caagctggcg gcgagcaatc tgcaggcggc ctgagcgccc gtcagggcgc agggaaggcg
     6061 gtcctcggga ccgccttttt tttggcaaac tcgcgtacgg catgaagcca tgagcgcaac
     6121 atcccttccc ctcctgtcga gcgtcgaagc gcgcgtgctg ggcgtcctgg tcgaaaagca
     6181 gcgcgcggtc ccggacacct atccgctgac gctcaacgcg ctggtgtccg gctgcaatca
     6241 aaagagcagc cgcgatccgg tgatggacct cgacggtgcg caggcgcagg aagcactcga
     6301 cagcctcagg cgcctcacgc tggtcatcga atcgagcggc gggcgggtgc cgcgctacga
     6361 gcacaacatg gagcgcgtgc tgcagatacc gacgacctcg gcggcgctgc tggccgtgct
     6421 gatgctgcgc gggccgcaga ccgcgggcga gctgcgcatc aactgcgacc ggctgcacaa
     6481 gttcgcggat atctccgccg tcgagggctt cctgcacgag cttgccgaac ggcccgccgg
     6541 cgcgctggtg gcgcagctgc cgcgacagcc gggctcgcgc gagaaccgct gggcgcattt
     6601 gctgtgcggc gcgcccgccg ccgagccgca cgcggccccg gcggccgcgc cccgcgccga
     6661 ggcgggcggt gaactcgcgt ctttgcgggg ccgggtggag gccctggaag ccagcgtggc
     6721 cgaattgcgc gcgctggtgg cggcactgca atccgcgcgg gattgagacg ccgcgcgccg
     6781 atgcgcaagg aattgcgcat ccggaaagcc cgcgcgcgcg aaaccaatcg ctatcttgcc
     6841 cccgcgccga gcgcgcgccg cgcgagctcc tcgacgttcg ccgccgaggg cgcgcgccgc
     6901 ccctgctcga tgtcgtgaac cagcgcggtc gccgcgtcgc accagggcgt ggcgatgccg
     6961 tgcgcgcgcc ccttgcgcgc cgccaggccg ctgaagtagt cgacctcggt gcggcgcccg
     7021 cgcagcacgt cctgcagcag cgaggtggct gcgtccgcgc tcacgcgtgc ggcgcgcgcg
     7081 gccagcccgc ggtcgaacgc ggtcgaggcg ccgagggcgc cggcgtagac ctcctcgtca
     7141 ctgaaatcgc ccatcacgcg cgcgacgcga tggccctcgg ccgccgccac gcgcagcact
     7201 tcggcggcga tgccggcaat ggcgcgccgc gcgccggcgc tcgcgagcat cgccgccgac
     7261 cccaagccgc ccaggccgga cacgggattg agcgcgctgt tggtcaccat cttcgtccag
     7321 cgctcgcgca gcaggtcgtc gatcggctcg gcgggccaca cggcctcgag caggcgcgca
     7381 acgcgccgcg cgttgtcgcc ggccgggcgc aggtactcgc cgacgtagac cccgggcaag
     7441 tgcactgcgg ccgcggcccg gctcaccgag gcagtgaccg tgatcagccc gggctcgagc
     7501 agcaccgcgt tgaccagcac gacgccgccc agcacgcgcc cggcgccgac ccgcggcgca
     7561 atctgctcct cgttgatcga attctgcagg ctcacgacca gcgcatccgg cgccaggtgc
     7621 ggcagcacgc ggcgcagcgc agcttcggtg tcgtaggact tgaccgccag caggcacagg
     7681 tcgacctgcc cctcgagctc gtgcggtgcg atcaccggca gtgggtagcg ctccacgccg
     7741 ccctcggcgc cggcgatgcg caatcccccg cgcaccaccg cctcgcgctg cgcgtcccac
     7801 ggctccacgg cggtcagcgc cacgcccgcg ccgtgcagcc tgccggcgac ctggccaccg
     7861 atcgcgccga tgccatacac gaccagccgc ccgcccatat cagaaggcgc cgccctgctt
     7921 cctgctgtcg acgatgccct gcgcccgcat gctgatctgg ctttgcaccg cgatgttgag
     7981 cagcgccggc ttgcccgcgg cgagcgcgcg ctcgagcgcg ggcgcgagct gctcgggccg
     8041 ctcgaccagc gcgccgtgcg cgcccaggcc ctgcgccacc aggtcggagc gcgccggcgc
     8101 gaggtcggtg accacggcct tgccgtacag gcc
//
LOCUS       DBNHKE010000236        12785 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1315612, whole genome shotgun sequence.
ACCESSION   DBNHKE010000236 DBNHKE010000000
VERSION     DBNHKE010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1315612"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="AC16O9_16170"
     CDS             <1..557
                     /locus_tag="AC16O9_16170"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase family
                     protein"
                     /protein_id="HIR6518193.1"
                     /translation="CAAARRLAPARFDLESMELLSLTLSDTLPAAALFERHRAFGARL
                     EAAVPARRAPHAQSANPDRRLRVGYVSCDFNRHPVAWFLKPLLERHDRAGYEAICYFT
                     GDKRDEVTEEVRAAADGWREATALTDAQLAQLVEEDGIDVLVDLTGHAGALRLGAFAR
                     QPPPCRPAGSAISAARGSRASSTG"
     gene            494..1282
                     /locus_tag="AC16O9_16175"
     CDS             494..1282
                     /locus_tag="AC16O9_16175"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase family
                     protein"
                     /protein_id="HIR6518194.1"
                     /translation="MQASWLGYLGSTGLTRIQYRLTDARADPPGEADRLHTETLVRLP
                     HSLWCYRPVHDAPHAAAPPHMAQGHATFGSFNHAPKLSAAARRLWAEILRGAPGARLL
                     IVGFPEGRARRALLDEFGAAGVEASRLTVLPRVSFGEYLRQFDAVDLALDSLPYGGGT
                     TTFDTLWMGVPVLTLAGERSAGRSSASILGALGLDDWVATTPEDYVRLALAHAADGSG
                     LAHLRATLRARLRRSPLMDEAGFARDMESAYRVMWRTWCAQRIT"
     gene            1314..1646
                     /locus_tag="AC16O9_16180"
     CDS             1314..1646
                     /locus_tag="AC16O9_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518195.1"
                     /translation="MFGNWFNTKEVDEVADAIVADLVKRLPPSPGPHTRKTADRLRKT
                     HDVIFARLEQFARSRRLNIYKKARLGNRVRWALREAGYAPEFTDALSYELVTVATLAS
                     RQAAKTSG"
     gene            1655..4108
                     /locus_tag="AC16O9_16185"
     CDS             1655..4108
                     /locus_tag="AC16O9_16185"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6518196.1"
                     /translation="MLGWLLGKRRAAAPARGAREVLVEAFAHHQAGRLAEAQAGYQAV
                     LALDARNFDAVNLLGVLALQSARPGEAAEHFARAADIAPQNAAAHNNLGEAYRRLGRT
                     AEAIESLERAVAQDANYAEAHFNLGGVLRRAGRAEEARAAFARAATLKPAFTEAHLRL
                     GETLNALGRPEEAAVALRAALELDPELAEAHSSLGTALRDAGHLDEALASCQRAVALK
                     PESALAHLNVGNVLRDKGFFDEAIAAYRSATTLDPDFAEAHNNLGNALRDRHRLDEAL
                     ASFERALRLNPGLMEAWLNVGSALCRQRRFADAAEVLRGALARKPEFAEAHLELGNAL
                     MAMGEQAEALECYRAALVHDPESEVARWSLAMSQLPAIADSAAEQALARDAFAAELDA
                     LEGWCESRSAERTARAVAVQQPFYLAYLEGDHHELLARYGRVSCRLIGNWQNAAGLAA
                     PSRITRAEIRVGIVSAHLHDHSVWNAIVKGWLSRIDRTRFDIRLFHLGVANDAETAIA
                     KTLATHYSYGRTDWREWAPLILEHQPDVLIYPEIAMDPTTAKLAAMRLAPVQAAAWGH
                     PITTGLPSIDYFLSADALEPAAGAEHYTEKLVRLPGLGVCLEAQKTVAREVDPAALGI
                     RDGVPLLLCPGTPFKYTPRHDAVLVEIARRLGECQFLFFTPHPPELAARLRRRMERAF
                     AQAGLDASAFVTWVPWQDRASFHGLMAQADAHLDTLGFSGFNTALQSIEAGLPVVAWE
                     GRFLRGRLASGILRRMGLDELVATSEAEYAQLAVKLAQDAAYWGEVRERLVAARERLY
                     GDAEPVRALERFLEDAVRK"
     gene            complement(4098..6914)
                     /locus_tag="AC16O9_16190"
     CDS             complement(4098..6914)
                     /locus_tag="AC16O9_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6518197.1"
                     /translation="MDQASGPIDAGKVLAVVHAVAREARPSIEPHVTLDSSLEKELGL
                     DSLARVELVLRLERQFATSLPEQALATSETPRDLLRFLLSSAGHAPRAADLSVASLAH
                     GTDVRPPVKAQTLTEALEYHVERQPERLTVHLYEDGQETPITYRQLYDGALGYAARLA
                     EAGLQPGQMVAIMLPTCKEYLYSFYGVLLAGGVPVPLYPPARLTTIEDHMTRHVGILK
                     SAGATIMITIPEAKALAYLLRAQVESLRAVMMPADLQGSGAGFAPVRGRSGDIGFLQY
                     TSGSTGSPKGVVLSHANLLVNVQAMGKAVSAGPDDVFVSWLPLYHDMGLIGGCFATMF
                     LGFPVVLMSPLAFLSRPGSWMRAIHRHRGTISGGPNFSYELCLRRIADEEMEGIDLSS
                     WRFAFNGAEPVSPDTITAFADRFSRWGFRRNSLSPVYGLAECTVGVSFTTPGEPWRMD
                     ALDREVFSQTGEAVPAKAADPSPLKVVGCGNVIEGHDLRVVDAAGLELPDGQEGLLQF
                     RGPSATSGYYRNPAATKSLFDGEWVNTGDRAYLKEGMVYITGREKDIIIRGGRNISPY
                     ELEQAVGDLGGVRRGCVAVFGSVDPASGTERLVVLAEMRENDPSRHDDLRRMINELAV
                     SLVGAPADDIVLAPPATVPKTSSGKIRRVAAREFYERGPTSVKPQAVWLQFARLVLAG
                     AAPQLRRTWRTVRGALYALRVYVAFGLLFPFALLTAALAGPRITWRVGGAVCRAFLRA
                     AGIPLVVRGADALPSGPAVLAVNHTSYLDAVVLVAVLSPRVHAFVAKREFESNLLMRL
                     LLKGFGTIFVERFDVQKSAEHADVLVEAARAGASIVIFPEGTLTRNTGLMAFRAGAFQ
                     VAAQAGVPVVPVALRGVRSVLRDGTWYARRAPIAVTLSAPIAPDGDDWAATLRLRDRA
                     RAEILKHCGEPDLARALPAQDATS"
     gene            6942..8009
                     /locus_tag="AC16O9_16195"
     CDS             6942..8009
                     /locus_tag="AC16O9_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6518198.1"
                     /translation="MPTVDAARPFLERLDDAARNLLLSVARPVSFTRGARLVRFGEAT
                     RGAYVLREGTAEASVVLPGGDRLTVAKLGAGGLLGETALFERGTCTATVIAATNVDGW
                     FLDRDEFRALAAQRTPEALRLQHALTLLLAEKLRALNARVMDVATPEDRPPAKGAGKD
                     PLEGVKRSRKSTFDLQPFLPLLPLFEGFDEQEIAEVLDASKFVELPPGHGVFTIGQPS
                     EAAYAVLRGAVEIVARHAKRERRMAILGPGQLFGFMSLLAGGAHGSAATVREAAILLE
                     MPRAGFEALYFGASTVTTALHHAIQRSLLASLAQTNRHFTRLISLARLRGARREGDKL
                     ETVQGGQIVAAPAPASAAPAA"
     gene            complement(7987..8910)
                     /locus_tag="AC16O9_16200"
     CDS             complement(7987..8910)
                     /locus_tag="AC16O9_16200"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR6518199.1"
                     /translation="MCRALLYLGQPVPLDDFLFRPDSALVKQSYMPKMLYLLNLAGFG
                     MRAWDAASHEPDKPFAYASDAIPVFDRNLKGLAEKIRARCLLAHVRGVPYNTRVEIAT
                     QNVHPFQFPGVPLAMAHNGDLARFADMKPLLLPHLRPEFAAQIRGTTDSEWIYALVVS
                     RLKDPAARPSAEELHDAVAHTLGVLRAAREKLGIALASPVNLFLTDGMQLAAVRYTLD
                     FGCYPQDPAEFLPGHLAYYSLWYTVGRDYGLHDGEWRMTGGSDSADSVLVASEPLTRD
                     VSAWVEVPEYTMLHALIRRNQQPAITLRELR"
     gene            8984..10633
                     /locus_tag="AC16O9_16205"
                     /pseudo
     CDS             8984..10633
                     /locus_tag="AC16O9_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651778.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
     gene            10633..>12785
                     /locus_tag="AC16O9_16210"
     CDS             10633..>12785
                     /locus_tag="AC16O9_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6518200.1"
                     /translation="MDRVSTQQLATLPIEFKLNGRSVVGRPDETLIETAKRHGVEIPH
                     LCYTPGLRPDGNCRACVVEIKGERVLAPSCCRYPTEGMEVTSDSARAVLSQKMVLELL
                     LSDMPEKQYRKDSELDQWARKLDMGLPRFKARRQPAADITHPAIAVNLDACIQCTRCL
                     RACREEQVNDVIGYAWRGEHSKIVFDFDDPMGASTCVACGECVQACPTGALMPAREAG
                     LQQIDKTVDSVCPFCGVGCLLTYHVKENVIQFVNGKDGPSNHGRLCVKGRYGFDYVQH
                     PHRLTTPLIRKPGVKKSADVKVDPGSWGEVFREATWEEALDLAATGLARIRDADRTAL
                     AGFGSAKGSNEEAYLFQKLVRMGFGTNNVDHCTRLCHASSVAALLEGIGSGAVSNQVN
                     DVEHSEVIFLIGANPTSNHPVAATWLKNAAKRGAKLIVADPRRTDLSRHASHFLQFNP
                     DTDVALLNAMIHTIIDEGLANEAFIKDRTSGYDALKDNAKAFSPEKMAPICGIPAATI
                     REVARLYATSKASIILWGMGISQHVHGTDNARCLIALTLMTGQVGRPGTGLHPLRGQN
                     NVQGASDSGLIPMMYPDYQRVATPEAKARFEKFWGAELDPKPGLTVVEIMHAAYDGKI
                     RGMYIMGENPAMSDPDLAHARAAMAKLEHLVVQDIFLTETAYLADVVLPATAWPEKDG
                     TVTNTDRMVQLGRKALDAPGEAKPDLWIIQALARRL"
BASE COUNT         1923 a         4487 c         4577 g         1798 t
ORIGIN      
        1 cttgcgccgc cgcgcgccgg ctcgcgccgg cgcgcttcga cctggagtcg atggagctgc
       61 tgtcgctcac gctgtccgac acgctgccgg ccgcggcgct gttcgagcgc caccgcgcct
      121 tcggcgcgcg cctggaggcc gcggtgccgg cgcgccgcgc gccgcacgcg cagtccgcga
      181 acccggatcg gcgcctgcgc gtcggctacg tgtcgtgcga cttcaaccgc caccctgtgg
      241 cctggtttct caagccgctg ctcgagcgcc acgaccgcgc gggctacgag gcgatctgct
      301 acttcaccgg cgacaagcgg gacgaggtga ccgaggaggt gcgcgccgcc gccgacggct
      361 ggcgcgaggc gacggcgctg acggacgcgc agctcgcgca gctggtggaa gaggacggca
      421 tcgacgtgct ggtcgatctc accgggcacg ccggtgcgct gcgcctcggc gcgttcgcca
      481 ggcagccgcc cccgtgcagg ccagctggct cggctatctc ggcagcacgg ggctcacgcg
      541 catccagtac cggttgaccg acgcgcgtgc cgacccgccg ggcgaggccg atcgcctgca
      601 caccgagacg ctcgtgcgcc tgccgcacag cttgtggtgc taccgcccgg tgcacgacgc
      661 gccgcacgcc gccgcgccgc cgcacatggc gcagggccac gcgacctttg gctcgttcaa
      721 ccacgcgccc aagctctcgg ccgcggcgcg ccgcctgtgg gccgagatcc tgcgcggcgc
      781 gccgggcgcg cggctgctga tcgtcggctt tcccgagggc cgcgcgcgcc gcgcgctgct
      841 cgacgaattc ggcgcggcag gcgtggaggc gtcgcgcctc acggtgctgc cgcgcgtgtc
      901 gttcggcgag tacctgcgcc agttcgacgc ggtggacctg gcgctcgatt ccctgcccta
      961 cggcggcggc accacgacct tcgacacgct gtggatgggc gtgccggtgc tcacgctcgc
     1021 cggcgagcgc tccgccgggc gcagctcggc gagcatcctc ggcgcgctcg gcctcgacga
     1081 ttgggtggca acgacgcccg aggactacgt gcgcctcgcg ctcgcgcacg cggcggacgg
     1141 ctcgggcctc gcgcacctgc gcgccaccct gcgcgcgcgg ctgcggcgat cgccgctgat
     1201 ggacgaagcg gggttcgcgc gcgacatgga aagcgcttac cgcgtgatgt ggcgcacatg
     1261 gtgcgcgcag cgcatcacgt agaatggcgc ctgcccaatt ctgcgaggcg tccatgttcg
     1321 gtaactggtt caacacgaag gaggtcgacg aggtggccga cgccatcgtc gccgacctcg
     1381 tgaagcggct gccgccgtcg cccggcccgc acacgcgcaa gaccgccgac cggctgcgca
     1441 agacgcacga cgtgatcttc gcgcgcctgg agcagttcgc gcgctcgcgc aggctcaata
     1501 tctacaagaa ggcgcgcctc ggcaaccgcg tgcgctgggc actgcgcgag gcgggctacg
     1561 cgccggaatt caccgacgcg ctcagctacg agctggtcac cgtcgccacg ctggcgtcgc
     1621 gccaagcggc caagacgagc ggctgacgcc gccgatgctc ggctggttgc tcggcaagcg
     1681 gcgcgccgcg gcgcccgcgc ggggcgcgcg ggaggtgctg gtcgaggcgt tcgcgcacca
     1741 ccaggccgga cgcctcgccg aggcgcaggc cggctaccag gcggtgctcg cgctcgacgc
     1801 gcgcaacttc gacgcagtga acctgctcgg cgtgctcgcc ctgcagtcgg cgcgtcccgg
     1861 ggaggcggcc gagcacttcg cgcgcgcggc cgacatcgcg ccgcagaacg cggcggcgca
     1921 caacaacctc ggcgaggcct accggcgcct cggccgcacc gcggaagcga tcgagagcct
     1981 cgagcgggcg gtggcgcagg acgccaatta cgcggaagcg catttcaatc tcggcggcgt
     2041 gctgcgccgc gccggccgcg ccgaggaagc gcgcgcggcc ttcgcccgtg ccgcaacgct
     2101 gaagccggcg ttcaccgagg cgcacctgcg actgggcgag acgctgaacg cgctcgggcg
     2161 cccggaggag gcggcggttg cgctgcgcgc ggcgctcgag ctcgacccgg agcttgccga
     2221 ggcgcacagc agcctcggca ccgcgctgcg cgacgccgga catctcgacg aggcgctcgc
     2281 ctcctgccag cgcgcagtgg cgttgaagcc cgagtcggcg ctcgcgcacc tcaacgtcgg
     2341 caacgtgttg cgcgacaaag gtttcttcga cgaggcgatc gccgcctacc gcagtgcgac
     2401 cacgctggat cccgacttcg cggaggcgca caacaatctg ggcaatgcgc tgcgcgaccg
     2461 ccaccgcctg gacgaggcgc tcgccagctt cgagcgggcg ctgcgcctca accccgggct
     2521 catggaagcc tggctcaacg tcggcagcgc gctctgccgg cagcggcgct tcgccgacgc
     2581 cgcggaagtg ctgcgcggcg cgctcgcacg caagcccgaa ttcgccgagg cgcacctcga
     2641 gctgggcaat gcgctgatgg ccatgggcga gcaggccgag gcgctcgagt gctaccgcgc
     2701 cgctctcgtg cacgacccgg aatcggaggt ggcgcgctgg tccctcgcca tgtcccagct
     2761 gccggcgatc gccgacagcg cggccgagca ggcgctcgcg cgcgacgcat tcgccgcgga
     2821 gctggacgca ctcgagggct ggtgcgagtc gcgcagcgcg gaaaggaccg cgcgcgcggt
     2881 ggccgtgcag cagccgttct acctcgcgta cctggagggc gatcaccacg agctgctggc
     2941 gcgctacggg cgagtgtcct gcaggctgat cggcaactgg cagaacgccg ccggcctcgc
     3001 cgcgccctcg cgcatcacgc gcgccgagat ccgcgtcggc atcgtctccg cgcacctgca
     3061 cgaccattcg gtgtggaacg ccatcgtcaa gggctggctc tcgcgcatcg accgcacccg
     3121 cttcgatatc cggctgttcc acctcggcgt ggccaacgac gccgagaccg cgatcgccaa
     3181 gacgctggca acgcactact cgtacgggcg taccgactgg cgcgaatggg cgccgctgat
     3241 cctcgagcac cagcccgacg tgctgatcta ccccgagatc gccatggacc cgaccaccgc
     3301 caagctcgcg gcgatgcgcc ttgcgccggt gcaggcggcg gcctggggcc acccgatcac
     3361 taccgggttg ccgagcatcg attacttcct ctccgccgac gcgctcgagc cggccgcggg
     3421 cgccgagcac tatacggaga agctggtgcg gctgccgggg ctcggcgtat gcctggaggc
     3481 gcagaagacc gtggcgcgcg aagtggaccc ggcagcgctg ggcatccgcg acggcgtgcc
     3541 cctgctgctg tgccccggca cgcccttcaa gtacacgccg cgccacgacg cggtgctggt
     3601 ggagatcgcg cggcgcctgg gcgagtgcca gttcttgttc ttcacgccgc acccgcccga
     3661 gctcgccgcg cggctgcgcc gccgcatgga gcgcgcgttc gcgcaggccg ggctcgacgc
     3721 ttcagccttt gtgacctggg tgccctggca ggatcgcgcg tcgtttcacg ggctgatggc
     3781 gcaggccgac gcgcacctgg atacgctcgg gttttccgga ttcaacaccg cgctgcagtc
     3841 gatcgaggcg ggcctgccgg tcgtggcctg ggagggacgg ttcctgcgcg gccgcctggc
     3901 gagcggcatc ttgcgccgca tggggctcga cgagctggtg gcgaccagcg aagccgaata
     3961 cgcgcagctc gccgtgaagc tggcgcagga cgcggcctac tggggcgaag tgcgcgagcg
     4021 cctggtcgcg gcgcgcgaac ggctctacgg cgacgccgag ccggtgcgcg cgctggagcg
     4081 cttcctcgag gacgcggtca ggaagtagcg tcctgcgccg ggagcgcgcg cgccaggtcg
     4141 ggctcgccgc aatgcttgag gatctcggcg cgcgcgcggt cgcgcagccg cagcgtcgcc
     4201 gcccagtcgt cgccgtcggg cgcgatcggc gcgctcaatg tcacggcgat cggcgcacgc
     4261 cgcgcgtacc aggtgccgtc gcgcagcacc gagcgcacgc cgcgcaacgc gaccggcacc
     4321 accggcaccc cggcctgcgc cgccacctgg aacgcgccgg cgcggaacgc catcagcccg
     4381 gtgttccgcg tcagcgtgcc ctccggaaag atgacgatgg aggcgccggc gcgcgcggcc
     4441 tcaacgagga cgtcggcgtg ctcggcgctc ttttgcacgt cgaagcgctc gacgaagatc
     4501 gtgccgaagc ccttgagcag cagccgcatc aggagattgg actcgaactc gcgcttggcg
     4561 acgaacgcgt gcacgcgcgg cgaaagcacc gccaccagca ccaccgcgtc gagatagctc
     4621 gtgtggttca ccgcgagcac cgccgggccc gacggcagcg cgtcggcgcc gcgcacgacg
     4681 agcggaatgc ccgcggcgcg caggaaggcg cggcataccg ccccgccgac gcgccaggtg
     4741 atgcgcggcc cggcgagcgc cgcagtcagc agggcgaagg gaaataacag cccgaaggcg
     4801 acgtagacgc gtagggcata cagcgcgccg cgcaccgtgc gccaggtgcg gcgcagctgc
     4861 ggcgccgcgc cggcgagcac cagccgcgcg aactgcagcc ataccgcctg cggttttacc
     4921 gacgtcgggc cgcgctcgta gaactcgcgc gccgccacgc gccggatctt gccgctcgag
     4981 gtcttgggca cggtggcagg gggcgcgagc acgatgtcgt cggccggcgc gccgaccagg
     5041 ctcacggcga gctcgttgat catgcgccgc aggtcgtcgt ggcgcgacgg gtcgttttcg
     5101 cgcatctccg cgagcaccac gaggcgctcg gtgccgcttg ccgggtcgac gctgccgaac
     5161 accgcgacgc agccgcggcg cacgccgccg aggtccccca ccgcctgctc cagctcgtag
     5221 gggctgatat tgcgcccgcc gcggatgatg atgtcctttt cgcggccggt gatgtacacc
     5281 atgccttcct tcaggtaggc gcggtcaccc gtattcaccc attcgccgtc gaacaggctc
     5341 ttggtcgcgg ccgggttgcg gtagtagccc gacgtggccg atgggccgcg gaactggagc
     5401 agcccctcct ggccgtcggg cagctcgagc cccgcggcgt ccaccacgcg caggtcgtgg
     5461 ccttcgatca cgtttccgca gcccaccacc ttgagcggcg acggatcggc cgccttcgcc
     5521 ggcaccgcct cgccggtctg cgaaaagacc tcgcggtcga gcgcgtccat gcgccacggc
     5581 tcgcccggcg tggtgaacga gacgccgacg gtgcattcgg cgaggccgta caccggcgaa
     5641 agggagttgc ggcgaaagcc ccagcgcgag aacctgtccg cgaacgcggt gatcgtgtcc
     5701 gggctcaccg gctcggcgcc gttgaacgcg aagcgccacg aggacaggtc gatgccttcc
     5761 atttcctcgt cggcgatgcg gcgcaggcac agctcgtagg agaagttcgg tccgccggaa
     5821 atggtgccgc ggtggcggtg gatggcgcgc atccagctgc cgggccgcga caggaaggcg
     5881 agcggcgaca tcagcaccac cgggaagccg agaaacatgg tggcgaagca gccgccgatg
     5941 aggcccatgt cgtggtagag cggcagccag ctcacgaaca cgtcgtcggg gccggcgctg
     6001 accgccttgc ccatggcctg aacgttgacc agcaggttgg cgtgcgacag caccacgcct
     6061 ttcgggctgc ccgtgctgcc ggaggtgtac tgcaggaaac cgatgtcgcc ggatcttccg
     6121 cgcaccgggg cgaacccggc gccgctgccc tgcaggtccg cgggcatcat gaccgcgcgc
     6181 aggctctcca cctgtgcgcg cagcagatag gcgagcgcct tcgcctcggg aatggtgatc
     6241 atgatggtcg cgccggcgct cttcaggatg ccgacgtggc gcgtcatgtg gtcctcgatg
     6301 gtggtgaggc gcgccggcgg gtacagcggc accggcacgc cgcccgcgag cagcacgccg
     6361 tagaaggaat agaggtattc cttgcaggtg ggcagcatga tcgcgaccat ctgccccggc
     6421 tgcagcccgg cctcggcgag ccgcgcagcg tagccgagcg cgccgtcgta gagctggcgg
     6481 taggtgatgg gcgtttcctg gccgtcctcg tacagatgca ccgtgaggcg ctcgggctgg
     6541 cgctcgacgt ggtactcgag cgcctcggtg agcgtctgcg ccttgaccgg cgggcgcacg
     6601 tcagtgccgt gcgccaggct ggcgacgctc agatccgcgg cgcgcggggc gtggccggcg
     6661 ctcgagagca ggaagcgcaa caggtcgcgc ggcgtttcac tcgtggcgag cgcctgctcc
     6721 ggcaggctgg tggcgaactg gcgttccagc cgcaggacga gctcgacgcg cgccagactg
     6781 tccaggccca gttccttctc cagcgagctg tccagcgtga cgtggggctc gatgctcggg
     6841 cgggcctcgc gcgcgacggc gtgcaccacc gccagcacct ttcccgcgtc gatcggtccg
     6901 ctggcctgat ccatggtcgg ccgagtgtac actcgtcggc gatgccgacc gtcgatgccg
     6961 cgaggccgtt tctcgagcgc ctggatgatg cggcgcgcaa tctgctgctc tcggtcgcgc
     7021 ggccggtgtc cttcacgcgc ggcgcgcgcc tggtgcgctt cggcgaggcg acgcgcggcg
     7081 cctatgtact gcgcgagggc acggcggaag cgagcgtggt gctgccgggc ggcgaccgcc
     7141 tcacggtggc gaagctcggc gcgggcggcc tgctgggcga aacggcgctg ttcgagcgcg
     7201 gtacgtgcac ggctaccgtg atcgccgcga ccaatgtgga cggctggttt ctcgaccgcg
     7261 acgagttccg cgcgctcgcg gcgcagcgca ccccggaggc gctgcgcttg cagcacgcgc
     7321 tcacgctgct gctggcggaa aaactgcgtg cgctcaacgc gcgcgtcatg gatgtggcga
     7381 cgccggagga ccggccaccg gcgaagggcg ccggcaagga tccgctcgaa ggcgtgaaac
     7441 gctcgcgcaa gagtactttc gacctgcagc cgttcctgcc gctgctgccg ctgttcgagg
     7501 gcttcgacga gcaggagatc gcggaggtgc tcgatgcgtc gaagttcgtc gagctaccgc
     7561 ccggccacgg ggtgtttacc atcgggcagc cgtcggaggc ggcgtacgcg gtgctgcgcg
     7621 gtgccgtcga gatcgtggcg cgacacgcca agcgcgagcg gcgcatggcg atcctcgggc
     7681 cgggccagct gttcggcttc atgagcctgc tcgcgggcgg cgcccacggc tcggcggcga
     7741 cggtgcgcga ggcggcaata cttctcgaga tgccgcgcgc ggggttcgag gcgctgtact
     7801 tcggcgccag caccgtgacc accgcgctgc accacgcgat ccagcgcagc ctgctcgcgt
     7861 cgcttgcgca gaccaaccgc cacttcaccc gcctgatcag cctggcgcgg ctgcgcggcg
     7921 cgcgccgcga gggcgacaag ctcgagaccg tgcagggcgg ccagatcgtg gccgcgccgg
     7981 cgcccgctag cgcagctccc gcagcgtgat cgccggctgc tgattgcgcc ggatgagcgc
     8041 gtgcagcatc gtgtactcgg gcacctcgac ccaggccgag acgtcgcgcg tcagcggctc
     8101 ggaggcgacc agcaccgagt cggcgctgtc gctgccgccg gtcatgcgcc actcgccgtc
     8161 gtgcagcccg tagtcgcgcc cgaccgtgta ccagaggctg tagtaggcga ggtgcccggg
     8221 caggaactcc gccggatcct gcgggtagca gccgaagtcg agcgtgtagc gcacggccgc
     8281 gagctgcatg ccgtcggtga ggaacaggtt gacgggcgag gcgagcgcaa tgccgagctt
     8341 ttcgcgcgcc gcgcgcagca cgcccagcgt gtgcgccacc gcgtcgtgca gctcctcggc
     8401 cgagggccgc gccgccgggt ccttgaggcg cgacacgacg agcgcgtaga tccattcgct
     8461 gtcggtggtg ccgcggatct gcgccgcgaa ttccgggcgc aggtgcggca gaaggagcgg
     8521 cttcatgtcg gcgaagcgcg cgaggtcgcc gttgtgcgcc atggcgagcg gcacgcccgg
     8581 gaactggaac gggtgcacgt tctgcgtcgc gatctcgacc ctggtgttgt aaggcacgcc
     8641 gcgcacgtgg gccagcaggc agcgggcgcg gatcttttcg gccagcccct tcaggttgcg
     8701 gtcgaatacc gggatggcgt ccgaggcgta ggcgaagggc ttgtccggct cgtgcgacgc
     8761 cgcgtcccag gcgcgcatgc cgaagccggc gaggttgagc aggtacagca tcttcggcat
     8821 gtagctttgc ttgaccagcg ccgaatcggg gcggaacaga aaatcgtcca gcggcaccgg
     8881 ctggccgagg tagagcagcg cgcggcacat ggttcgccaa tggtcccgaa ccccttgacc
     8941 gcagtcaaga aagcgagacg cacggctgcc ggataatggc cgcatgaaag tccgtccgac
     9001 gcccaagggg cgcagggtcg agccgcaggc gctgcaggag gtcagggcgc tgctcggcac
     9061 atcgccgcgc cgtcccgacc tgctgatcga gcacctgcat cgcatccagg accgctacgg
     9121 acacctttcc gccgcgcgcc tcgcggcgct ggcgcaggag ctgaggctct cgctcaccga
     9181 agtgttcgag gtggcgacct tctatcacca cttcgacgtg gtgtaggagg gcgagcaggc
     9241 gccgcccgcg ctcaccgtgc gcatatgcga ttccatcgcc tgcgagatgg caggcgcgcg
     9301 ggagctcctc gagaaattgc cggccatcct cggcaaggac gtgcgcgtga ttcccgcgcc
     9361 ctgcgtcggg cgctgcgaag tcgcgccgtg cgccgtggtg caccagaatc ccgtgggcaa
     9421 cgccaccgtc gacaaggtgc gcgcggcggt gcagaaaaag tccttcaagt gcgcgcagcc
     9481 cgcgtacatc gattacgccg cgtatcgcaa ggcgggcggc taccgcctga tcgccgactg
     9541 ccttgccggc aagcacacgc gcgacgcgat caccacgatc atggaggact ccggcttgag
     9601 gggcctgggc ggcgcggggt tcccggccgg ccgcaagtgg cgcctggtcg ccgccgagcc
     9661 cgcgccgcgg ctgatggcgg tcaacatcga cgaaggcgag cccggcacgt tcaaggaccg
     9721 ctggtatttg gagcgcgacc cgcaccgctt cctcgagggc atgctggtgg cggcgtggtg
     9781 cgccggggtc ggcgagatct acgtctacct gcgcgacgag tacgccggct gccgcgcggt
     9841 gctggagcgc gagctggcca agctgcagaa gaacccgccg tgcgcgctgc cgccgatcag
     9901 tcttcgccgc ggcgcgggcg cctacatctg cggcgaggag tcggcgatga tcgagtcgat
     9961 cgaaggcaaa cgcggcatgc cgcgcctgcg accgccctac gtagcgcagg tgggcctctt
    10021 cggctacccg acgctcgagc acaacatgga gacggtgcac tgggtgcgcg agatcgtgga
    10081 gaaaggcgcc gcctggttct ccggccacgg ccgcaacggc cgcaagggcc tgcgctcgtt
    10141 ctcggtatcg gggcgggtga acaagcccgg catgcacctt gctcccgcgg gcgtcagcgc
    10201 gcgcgagctg atcgacgagt actgcggcgg catgctgccc gggcacgagt tctacgccta
    10261 cctgccgggc ggcgcctcgg gcggtatcct gcccgcgtcg aagggcggcg tgccgctcga
    10321 cttcgacacg ctgcagccgc acggctgctt catcggctcg gcggcgatcg tcatcctgtc
    10381 gcagcacgac caggcggcgg ccgcggcgcg caacctgatg cggttcttcc gcgaggagtc
    10441 ctgcggccag tgcacgccct gccgcgtcgg taccgccaag gcgctcgcgc tgatgcagga
    10501 aagaaagtgg aacgcgccgt tgctcggcga gctctcacag gcgatgatgg acgcctcgat
    10561 ctgcggcctg gggcaggcgg cgccgaaccc ggtgcagagc gtcatcaaat actttccgca
    10621 cgaggtgcaa tgatggatcg cgtgagcacc cagcaactgg cgacgctgcc gatcgagttc
    10681 aagttgaacg gccggagcgt ggtcggccgg ccggacgaga ccctcatcga gaccgcgaaa
    10741 cgccacggcg tcgagatccc gcacctttgc tacacgcccg gcctgcggcc tgacggcaac
    10801 tgtcgcgcct gcgtggtcga gatcaagggc gagcgcgtgc tcgcgccctc gtgctgccgc
    10861 tacccgacag aaggcatgga agtgacgagc gactcggcgc gcgccgtgct gtcgcagaag
    10921 atggtgctcg aattgctgct gtccgacatg ccggaaaagc agtaccgcaa ggattccgag
    10981 ctcgaccagt gggcacggaa gcttgacatg ggcctgccgc gcttcaaggc gcgccggcag
    11041 cccgcggccg acattacgca cccggcgatc gcggtcaacc tcgatgcctg catccagtgc
    11101 acgcgctgcc tgcgcgcctg ccgcgaggag caggtgaacg atgtcatcgg ctacgcctgg
    11161 cgcggcgagc attcgaagat cgtgttcgac ttcgacgacc cgatgggcgc ctcgacctgc
    11221 gtcgcctgcg gcgagtgcgt gcaggcctgc cccaccggcg cgctgatgcc ggcgcgcgag
    11281 gcgggactgc agcagatcga caagaccgtg gattcggtgt gcccgttctg cggcgtcggg
    11341 tgcctgctga cctaccacgt caaggaaaac gtgatccagt tcgtcaacgg caaggacggc
    11401 ccgtcgaacc acgggcgcct gtgcgtgaag ggccgctacg gcttcgacta cgtgcagcat
    11461 ccgcaccgcc tgacgacacc gctcatccgc aagcccgggg tgaagaaatc ggcagacgtc
    11521 aaggtcgatc cgggtagctg gggcgaggtg tttcgcgaag cgacctggga agaggcgctc
    11581 gatctcgccg ccacgggcct cgcgcgcatc cgcgacgccg accgcacggc gctcgccggc
    11641 ttcggttccg cgaaaggctc gaatgaggag gcgtacctgt tccagaagct ggtgcgcatg
    11701 ggttttggca ccaacaacgt cgatcactgc acgcgcctgt gccacgcctc gtcggtggcg
    11761 gcgctgctgg aaggcatcgg ctcgggcgcg gtgtcgaacc aggtgaacga tgtcgagcac
    11821 tcggaagtca tcttcctcat cggcgccaac ccgacctcca accacccggt ggcggcgacc
    11881 tggctgaaga atgccgccaa gcgcggcgcc aagctgatcg tggccgatcc gcggcgcacc
    11941 gacctgagcc gccacgcgtc gcacttcctg cagttcaacc cggataccga cgtcgcgctc
    12001 ctgaacgcga tgatccacac catcatcgac gaaggcctgg cgaacgaagc gttcatcaag
    12061 gaccgcacca gcggctacga cgcgctgaaa gacaacgcga aggcgttctc gccggagaag
    12121 atggcgccga tctgcgggat tcccgcggcc accatccgcg aggtggcgcg gctgtacgcg
    12181 acctcgaagg cctcgatcat cctgtggggc atgggcatca gccagcatgt gcacggcacc
    12241 gacaatgcgc gctgcctcat cgcgctgacg ctgatgaccg gccaggtcgg gcgcccgggc
    12301 accggcctgc acccgctgcg tggccagaac aacgtgcagg gcgcttccga ctcgggcctc
    12361 atcccgatga tgtacccgga ctaccagcgc gtggcgacgc ctgaagcgaa ggcgcgcttc
    12421 gagaagttct ggggcgccga gctcgacccc aagcccggcc tcaccgtggt cgagatcatg
    12481 cacgccgcgt acgacggcaa gatccgcggc atgtacatca tgggcgagaa cccggcgatg
    12541 tcggacccgg atctcgcgca cgcgcgcgcg gcgatggcca agctcgagca cctggtggtg
    12601 caggacatct tcctgaccga gacggcgtac ctcgccgacg tggtgctgcc ggccaccgcc
    12661 tggccggaga aggacggcac cgtgaccaac accgatcgca tggtgcaact gggcaggaag
    12721 gcgctcgacg ctccgggcga ggcgaagccc gacctttgga tcatccaggc gctggcgcgg
    12781 cgcct
//
LOCUS       DBNHKE010000237         4417 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_522008, whole genome shotgun sequence.
ACCESSION   DBNHKE010000237 DBNHKE010000000
VERSION     DBNHKE010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_522008"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1004
                     /locus_tag="AC16O9_16215"
     CDS             39..1004
                     /locus_tag="AC16O9_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HIR6518201.1"
                     /translation="MRVLVTGCAGFIGMHCTLRLLERGDEVLGADNLSPYYDVTLKQA
                     RLQRLEAERSFRFERSDLADATACARLFEAARPEAVLHLAAQPGVRYSFENPSSYIQS
                     NLVAFANVLEACRRHAPRHLVYASSSSVYGANPKLPWSETDNVDHPISLYAATKKANE
                     LMAHVYSHLHALPATGLRYFTVYGPWGRPDMSPMLFARAILEGKPIQVFNHGDMKRDF
                     TYIDDAAEATLRVLDRPPVTKPPHRVLNVGNHQPVALLEYIARLEAALGKKAVKDMRP
                     MQPGDVPATYADTRALQDAVGFAPATPLAEGLARFAEWFKDYYRA"
     gene            complement(982..2856)
                     /locus_tag="AC16O9_16220"
     CDS             complement(982..2856)
                     /locus_tag="AC16O9_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6518202.1"
                     /translation="MRNTGRQFLIRSSLAFAHDVVAAGVAWLAAFWLRFELQMPPPEF
                     WEVAVTTLPAVLAVHAVAFWALGLYRGLWRYASLPDLQRILVAVAVAALAVPALFTLV
                     ALETPVPRSVYLIMPVLLIGAMCGSRLLYRAWKERTLLGMVRHPQAQPVLVLGAGSAA
                     SSLLRDLANSNQWRVVGLLDDDAANRGSAIQGVKILGALADIGAAAARFGVAQAIIAM
                     PGATHTQRRRAVDLCSAAGLEVMTVPSYADLVSGRMSVSALRNVELDDLLGRDPVALD
                     DAGLRGLIEGKCVLVTGAGGSIGAELCRQIARYAPSRLVLFELSEFALYAIEQEFRDR
                     FPHVAASAVIGDAKDEPRVRDTFARFRPQIVFHAAAYKHVPLMEGENASQAVANNVLS
                     TVVTARAAQAAGAQKFVLVSTDKAVNPTNVMGASKRLAELACQALAGAGGTQFVTVRF
                     GNVLGSTGSVIPRFREQIARGGPVTVTHPDIQRYFMSIPEAAQLVLQAALMGKGGEIF
                     VLDMGEPVRIVELARQLIRLSGLSEDDIRIEYTGLRPGEKLYEELLAEAETTLPTPHP
                     KLRVARASASGNGALVGEVIDWIAAVRDPDAETVRERLRVWVPEYAPAPGGAKRGNNP
                     "
     gene            complement(2853..3833)
                     /locus_tag="AC16O9_16225"
     CDS             complement(2853..3833)
                     /locus_tag="AC16O9_16225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR6518203.1"
                     /translation="MPALAPIAAFLVALLVARLLLTPAGRRIALDTPNERSLHAQPVP
                     RTGGIALAAGMAAACALVWPGLPVILAAAALLAAASFADDVIGLSTLARLALHLGAAT
                     AALAYDLEVTAPLLFIVLALALAWTTNLYNFMDGSDGLAGGMAVFGFGAYALAAYLFG
                     ALTLAALCASLAAAAAAFLVFNWHPARLFMGDVGSVPLGFLAGALGLQGWAIGAWPLW
                     FPILVFAPFMCDATLTLIKRLLRREPVWRAHREHYYQRLVRMGFGHRGAALIEYAAMA
                     TCAALALLVFREPAAVQGIALALAAVALAGVAAWIDRRWARFVRVSGAAA"
     gene            complement(3844..>4417)
                     /locus_tag="AC16O9_16230"
     CDS             complement(3844..>4417)
                     /locus_tag="AC16O9_16230"
                     /inference="COORDINATES: protein motif:HMM:NF013255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6518204.1"
                     /translation="ALGAGARRFVYLSSIKAAQPDDAYGRAKSSAEQALLQLDGLEPV
                     ILRPPLVYGPRVKANFLALMRAIDRGWPLPLASIRNRRSLIYVGNLVDAIIRCVEGPA
                     VRKSYAVSDGAPVSTPELCRALGRALGRPARLWPFPPALLRLAPGLARLVESQEADEH
                     AIREELGWRPPYSFEEGLRDTAAWYRARGG"
BASE COUNT          747 a         1593 c         1518 g          559 t
ORIGIN      
        1 ataaagcgtg cgcaaggtaa cctatgataa gagatctcat gcgcgtcctc gtcaccggct
       61 gtgcgggttt catcggcatg cattgcacgc tgcgcctgct cgagcgcggt gacgaggtgc
      121 tgggggcgga caatctgtcg ccctactacg acgtgacgct caagcaggcg cgcctgcagc
      181 gactcgaagc cgaacgcagc tttcgcttcg aacggtccga tctcgcggat gcgacggcat
      241 gcgctcgcct gttcgaggcg gcccggccgg aggccgtgct gcacctcgcg gcgcagcccg
      301 gcgtgcgcta ctccttcgaa aacccatcgt cctatatcca gtcgaacctc gtcgctttcg
      361 ccaacgtgct ggaagcgtgc cgcaggcacg cgccgcgcca tctggtctac gcctcgagct
      421 cgagcgtcta cggcgcgaac ccgaagctgc cctggtcgga aaccgacaac gtcgatcacc
      481 ccatcagcct gtacgccgcg accaagaagg ccaacgagtt gatggcgcac gtctacagcc
      541 acctgcacgc gctgccggca acggggctgc gctacttcac cgtgtacggg ccctggggcc
      601 ggcccgacat gtcgccgatg ctcttcgcgc gcgccatcct cgaaggcaag ccgatccagg
      661 tgttcaacca cggcgacatg aaacgcgatt tcacctatat cgacgacgcc gcggaagcga
      721 cgctgcgcgt gctcgatcgc ccgcccgtga caaagccgcc gcatcgcgtg ctgaatgtcg
      781 gcaaccacca gccggtggcg ctcctagagt acatcgcgcg gctcgaggcc gcgctcggca
      841 agaaggcggt gaaggacatg aggccgatgc agcccggcga cgtgcccgcg acctacgccg
      901 acacgcgcgc gctgcaggac gccgtgggtt tcgcccccgc gacgccgctc gccgagggcc
      961 tggcgcgctt cgccgagtgg ttcaaggatt attaccgcgc ttagcgccgc cgggcgccgg
     1021 cgcatactcc ggcacccaca cccgcaggcg ctcgcgcacc gtttcggcgt ccgggtcgcg
     1081 aaccgcggca atccagtcga tcacctcgcc caccagcgcc ccgttgccgc tcgcgctggc
     1141 gcgcgcgaca cgcagcttgg ggtgcggcgt gggtagcgtg gtctcggctt cggcgagcag
     1201 ttcctcgtag agcttctcgc cggggcgcag gcccgtgtac tcgatgcgga tgtcgtcctc
     1261 ggacaggccg gacagccgga tcagctggcg cgccagctcg acgattctca ccggctcgcc
     1321 catgtcgagc acgaagatct cgcccccctt gcccatcagc gctgcctgca gcacgagctg
     1381 cgccgcctcc gggatggaca tgaagtagcg ctgtatgtcg gggtgcgtga ccgtcaccgg
     1441 cccgccgcgc gcgatctgct cgcgaaagcg cgggatcacg ctgccggtgg agccaagcac
     1501 gttgccgaag cgcaccgtga cgaactgcgt gcccccggcg ccggcgagcg cctggcaggc
     1561 aagctcggca aggcgcttcg aagcgcccat cacgttggtc ggattgaccg ccttgtcggt
     1621 cgagacgagc acgaacttct gcgcgccggc ggcctgcgcg gcgcgcgccg tgaccacggt
     1681 cgagagcacg ttgttggcca cggcctgcga ggcgttctca ccctccatca gcggcacgtg
     1741 cttgtaggcc gcggcgtgga acacgatctg cggcctgaag cgcgcaaacg tgtcgcgcac
     1801 gcgcggctcg tccttggcgt cgccgatcac cgcgctcgcg gcgacgtggg gaaagcggtc
     1861 gcggaattcc tgctcgatgg cgtacagcgc gaactcggag agctcgaaga gcaccaggcg
     1921 cgaaggcgca tagcgcgcga tctgccggca gagctcggcg ccgatcgacc cgcccgcgcc
     1981 ggtgaccaga acgcacttgc cctcgatcag cccgcgcagg cctgcatcgt cgagcgccac
     2041 cggatcgcgc cccagcaggt cgtcgagctc gacgttgcgc agcgccgata cgctcatcct
     2101 gccggagacg aggtcggcat aggacggcac ggtcatgact tcgaggcccg ccgccgagca
     2161 cagatccacc gcgcgccggc gctgcgtatg cgtcgcgccc ggcatggcga tgatcgcctg
     2221 cgccaccccg aagcgcgccg cggccgcgcc gatatcggcc agcgcgccga ggatcttcac
     2281 gccctggatc gcgctgccgc ggttcgcggc gtcgtcgtcg agcaggccga cgacgcgcca
     2341 ttggttgctg ttggcgagat cgcgcaagag gctggatgcc gcggagccgg cgccgagcac
     2401 cagcaccggc tgcgcctgcg gatggcgcac catgccgagc agcgtccttt ccttccacgc
     2461 gcggtacagc aggcggctgc cgcacatggc accgatcagc agcaccggca tgatcaggta
     2521 caccgagcgc ggcaccggcg tttcgagcgc caccagcgtg aacagcgccg gcacggcgag
     2581 cgccgcgacc gcaaccgcga ccagaatgcg ctgcaggtcc ggaaggctcg cgtagcgcca
     2641 caggccgcgg tacaggccca gggcccagaa cgccaccgcg tgcaccgcca ggaccgcggg
     2701 cagcgtcgtc accgcaacct cccagaactc cgggggtggc atctgcagct cgaagcgcag
     2761 ccagaacgcc gcgagccagg ccaccccggc ggcgacgacg tcgtgcgcga aggcgagcga
     2821 ggagcggatc agaaactggc ggccggtgtt cctcacgccg cggctcccga gacgcgcacg
     2881 aaccgtgccc agcgccggtc gatccacgcg gcgacgccgg cgagcgcaac cgcggcgagc
     2941 gcaagcgcga tgccctgcac ggccgccggc tcgcggaaca ccagcagcgc aagggccgca
     3001 caggtcgcca tcgccgcgta ttcgatgagc gccgcgccgc ggtggccgaa gcccatgcgc
     3061 acgagacgct ggtagtagtg ctcgcggtgc gcccgccaca cgggctcgcg gcgcaacagc
     3121 cgcttgatca acgtcagcgt cgcatcgcac atgaagggcg caaagacgag gatcggaaac
     3181 cacagcggcc aggcgccgat cgcccagccc tgcaagccga gagcccccgc cagaaaaccg
     3241 agcggcaccg atcccacgtc gcccatgaac agtcgcgccg gatgccagtt gaacaccaga
     3301 aacgccgccg cggcggcggc gagcgaggcg cacagcgccg cgagcgtcag cgcaccgaaa
     3361 aggtacgccg ccagcgcata agcgccgaag ccgaacaccg ccataccgcc cgcaagaccg
     3421 tccgagccgt ccatgaagtt gtagagattc gtggtccagg cgagcgccag ggcgagcacg
     3481 atgaagagca gcggcgcagt gacttcgagg tcgtacgcca gcgccgccgt cgccgccccg
     3541 aggtgcaacg cgagccgcgc cagcgtcgac agcccgatca cgtcgtctgc gaaggacgcc
     3601 gcggcgagga gcgccgcggc agcgaggatc acgggcaggc ccggccagac gagcgcacat
     3661 gcggcggcca tgccggccgc gagtgcgatg ccgccggtgc gcggcaccgg ctgcgcgtgc
     3721 agcgagcgct cgttcggcgt atcgagcgcg atgcgtcggc ccgcgggcgt gagcagcagc
     3781 cgcgcgacca gcaaagcgac gagaaacgcg gcaatcggcg ccagcgcggg catgccgcgc
     3841 attctagccg ccccgcgcgc ggtaccacgc cgcggtatcc cgcaacccct cctcgaacga
     3901 gtaaggcggg cgccagccca gctcttcccg tatcgcgtgc tcgtccgcct cctgcgactc
     3961 cacgaggcgc gcgagcccgg gggcgagccg cagcagcgcg ggcggaaacg gccacagccg
     4021 cgccgggcgc ccgagcgcgc ggccgagcgc gcggcaaagc tcgggcgtgg acacgggcgc
     4081 gccgtcgctg acggcgtagc tcttgcggac cgcgggtccc tcgacgcagc ggatgatggc
     4141 gtccacgaga ttgccgacat agatgaggct gcggcgattg cggatgctcg cgagcggcaa
     4201 cggccagccg cggtcgatgg cgcgcatgag cgcgaggaaa ttggccttca cgcgcggccc
     4261 gtaaacgagc ggcgggcgca ggatcacggg ctcgagtccg tcgagctgca ggagcgcttg
     4321 ctcggcgctc gatttcgcgc ggccgtaggc gtcgtcgggt tgcgccgcct tgatcgagct
     4381 gagatacacg aaacggcgcg cgccggcgcc aagcgcg
//
LOCUS       DBNHKE010000238         9673 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1421341, whole genome shotgun sequence.
ACCESSION   DBNHKE010000238 DBNHKE010000000
VERSION     DBNHKE010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1421341"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="AC16O9_16235"
     CDS             complement(<1..1132)
                     /locus_tag="AC16O9_16235"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HIR6518205.1"
                     /translation="MVRLDVYALDAGGHGIARNAAGKVVFVEGALGGETVDAQVLRSK
                     PKFDTARTVAVLKAASGRRAPPCPWVENCGGCAIQHADTCTQVAAKQRWLEDCFERIG
                     KVKPESMLPPIYGEEWGYRRRARLSVRYVEKKGGAMVGFRERRSTFVADMRSCLVLPS
                     HVSALIPKLRALIGELSIRERVPQIEVAVGDEAVVLVFRHLQPFSEADLDLLRTFADA
                     ARVQVWLQPRGPDSAAPLHPGSPPALYYALPEFGVRIAFHPTDFTQVNHAVNRLLVSR
                     AVRLLDPQPGERIGDLFCGLGNFSLPLAARGAQVIGLEGAKALVERARANALANGLTA
                     QFEVANLFEPNLAPYGAFDKLLIDPPREGAMELVKALPEAWPR"
     gene            complement(1120..1896)
                     /locus_tag="AC16O9_16240"
     CDS             complement(1120..1896)
                     /locus_tag="AC16O9_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6518206.1"
                     /translation="MTPILAFDIETVPDVGGIRRLYALPESLPDREVAEIAFQKRRTQ
                     TGSDFLGPQLHRVAVISCVLREGEGIRVFSIGEPETPENEAIQRFFDGIEKLVPQLVS
                     WNGGGFDLPVLNYRALVHGVAAPKFWDQGEDDRDFRFNSYLGRYHARHLDLMDVLAMY
                     QPRNNAPLDEVAQLAGLPGKIGVGGAQVWAAYLEGRMAEIRDYCEADTANTYLLYLRF
                     QKLRGALTGPQYLKECALLRGALEKLKAPHWREFLTRWSG"
     gene            complement(1923..2822)
                     /gene="cysM"
                     /locus_tag="AC16O9_16245"
     CDS             complement(1923..2822)
                     /gene="cysM"
                     /locus_tag="AC16O9_16245"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HIR6518207.1"
                     /translation="MKRTVEDFIGETPLVRLQRLPGEDSARRGNVILAKLEGNNPAGS
                     VKDRPAISMVRRAEERGAIKKGDVLIEPTSGNTGIALAMAAAIRGFRMILIMPEHLSV
                     ERRQTMRAFGAEIILTPQKGGMEAARDLAEKMVREGKGVMLDQFANPDNPLAHYETTG
                     PEIWRDTGGKITHFVSSMGTTGTIMGCSRFFKEKNPNVQIVGCQPEEGAQIPGIRKWP
                     EAYLPRIYDKARVDRLEYVSQADAEEMTRRLAREEGIFAGISSGGATAVALRIAAELK
                     NAVIVTIICDRGDRYLSTGVFPD"
     gene            complement(2819..3802)
                     /gene="rfaD"
                     /locus_tag="AC16O9_16250"
     CDS             complement(2819..3802)
                     /gene="rfaD"
                     /locus_tag="AC16O9_16250"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HIR6518208.1"
                     /translation="MLHVITGAAGFIGSRLVAALNQRGVTEILAVDNLENVDKVPNLV
                     GCEIEDYLDKREFLARLEAGQFDGAVEAVLHQGACSDTMASDGRYVMDNNYRYSRALL
                     DWCQEEEVALVYASSASVYGAGKNGFREERECEAPLNVYGYSKFLFDQVVRRTLPEGA
                     AQIVGLRYFNVYGPNEAHKGRMASVAFHAWHQLAAEGRVKLFVGSGGFGDGEQRRDFI
                     HVDDVVRVNLHFLEHRGRSGIFNCGTGRAQTFNELAAAVINAARGTRASAAELAAQKL
                     IEYVPFPPQLAARYQSFTEADLTQLREAGYDGEFMDVADGVAAYVNERLQA"
     gene            complement(3802..4731)
                     /gene="rfaE1"
                     /locus_tag="AC16O9_16255"
     CDS             complement(3802..4731)
                     /gene="rfaE1"
                     /locus_tag="AC16O9_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HIR6518209.1"
                     /translation="MRRPATDKARVLVVGDVMLDRYWYGDATRISPEAPVPVVLWQRE
                     EERLGGAANVARNCAALGAPTTLLSVTGRDEAGERVARLLEAEGVTASLRRDPSVRTT
                     QKLRVVHKKQQLLRIDMETPPSKEVLASKLADFRKALPACDVVLLSDYGKGGLAHVAT
                     MIREGRRAKKTVLVDPKGDDYSRYRGASMITPNLKELEDVVGRWKDERDLAARAQALR
                     ARLRLDALLLTRGEGGMTLFRPGRTVRIKGEPRDVADVTGAGDTVIGVLAVMLAAGES
                     MESAARLANRAGGIKVTKFGTAVVTREELFGGS"
     gene            complement(4844..6022)
                     /gene="lapB"
                     /locus_tag="AC16O9_16260"
     CDS             complement(4844..6022)
                     /gene="lapB"
                     /locus_tag="AC16O9_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR6518210.1"
                     /translation="MEFEYWWLLALPLFFGLGWLAARIDIRHLVSESRALPRSYFKGL
                     NFLLNEQPDKAIESFIEVVKVDPETVELHFALGSLFRRRGEYDRAIRMHQNLVERPDL
                     GAEQKLAAAFELAQDYLKAGILDRAEELFARLETGAHAAEARRYLLEIFQQEKDWARA
                     IDMAGKLQDDSGESRAKQISQFLCELAASEATHSRPQEARKHLEAALEANRKSVRASI
                     SLGDLERAAGNLERAIEHWQRVESQNPAYLALVAPRLLDAYRQVARLEVGLTLLAGYL
                     ERYPSLDLLDTVFQTTLEAKGAEAAYKLVRDELRRNPTLLGLDRLLEAQIVADTHPER
                     RRDLELIRNLVHSHTRRLARYRCEECGFKARQFHWHCPACGSWESYPPRRTEEFDLSP
                     "
     gene            complement(6022..6318)
                     /locus_tag="AC16O9_16265"
     CDS             complement(6022..6318)
                     /locus_tag="AC16O9_16265"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6518211.1"
                     /translation="MRLLTWTIRIALFILLLAFAARNTETVTLRFYFDLAWQAPLVVL
                     LLAFFAAGAVLGLLAALGAVLRQRREAARLRRELRAVGARTHDAPTLPPPPAEG"
     gene            complement(6347..6664)
                     /locus_tag="AC16O9_16270"
     CDS             complement(6347..6664)
                     /locus_tag="AC16O9_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR6518212.1"
                     /translation="MTKSELIARLAQRYPQLVAKDAEYAVKMILDAMTQALLSGHRIE
                     IRGFGSFGLNYRPPRIGRNPKSGEKVHVPEKYVPHFKAGKELRERVDSAVEAAAQQAQ
                     ATQ"
     gene            complement(6702..8435)
                     /gene="rpsA"
                     /locus_tag="AC16O9_16275"
     CDS             complement(6702..8435)
                     /gene="rpsA"
                     /locus_tag="AC16O9_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR6518213.1"
                     /translation="MASVSHAVSKSPANESFAALFEESLTRKEMRIGEVITAEVVSVD
                     PNYVVVNAGLKSESIIAAEEFKNDAGEVEVKPGDFVSVSIEALEDGYGATRLSRDKAK
                     RMAAWIELEESLEKGEKIEGVITGKVKGGLTVMTKGIRAFLPGSLVDLRPVKDTTPYE
                     GKTMEFKVIKLDRKRNNVVVSRRAVLEASAGAEREALLSSLQEGAVVKGIVKNITDYG
                     AFVDLGGIDGLLHITDLAWRRVKHPSEVLNVGDEVTAKVLKFDAEKNRVSLGLKQLGE
                     DPWVGISRRYPQGTRLFGKVTNLTDYGAFVEVEAGIEGLVHVSEMDWTNKNVHPSKVV
                     QVGDEVEVMILEIDEERRRISLGMKQCKPNPWEDFAREFKKGDRVKGQIKSITDFGVF
                     VGLSGGIDGLVHLSDLSWSLAGEEAVKNFKKGEEVEAVVLAIDVERERISLGIKQLGG
                     DPFTNFIAGHDKNSVVPGTVKSVDAKGAVVALADGIEGYLRASEFARDRIDDLRAALK
                     EGDSVSAMIINVDRKNRSINLSVKAKDMAEETDAMKQLRSENQASASGSTNLGALLRA
                     KLDKGSSEPQK"
     gene            complement(8513..9157)
                     /gene="cmk"
                     /locus_tag="AC16O9_16280"
     CDS             complement(8513..9157)
                     /gene="cmk"
                     /locus_tag="AC16O9_16280"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR6518214.1"
                     /translation="MTVPVVAIDGPSASGKGAVAQQVAEALGFHCLESGALYRLVALA
                     GPEHPEDTARRMQVEFRGGRIFLSGQDVTESLREEGVGVRASDVAQRPAVRQALLERQ
                     RAFRRAPGLVAEGRDMGTVVFPDAPLKVFLTATPEVRARRRYKQLIDKGIDANLAALS
                     RDLAQRDARDVARAVAPLAPAPDALLLDSSDRSLDEVVGLILGWCRERNLGGRA"
     gene            complement(9154..>9673)
                     /locus_tag="AC16O9_16285"
     CDS             complement(9154..>9673)
                     /locus_tag="AC16O9_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6518215.1"
                     /translation="IAGGPVRVEGVGRASIQGDVRFTEVLEVMGAQVSMGEDWIEAEG
                     RAPLRAFDLDLNHIPDAAMTAAALALFADGPCVVRNIASWRVKETDRIAAMAAELAKL
                     GAGVEAGVDYLKVAPARLVSGASLDTYDDHRMAMSLSLAALGGVRVRINDPDCVAKTF
                     PGYFAAFAAIAR"
BASE COUNT         1450 a         3398 c         3240 g         1585 t
ORIGIN      
        1 gccgcggcca cgcctcgggc agcgccttga ccagttccat ggccccctcg cgcggcgggt
       61 cgatcagcag cttgtcgaag gcaccataag gcgcgagatt cggctcgaaa aggttggcga
      121 cctcgaactg tgcggtaagg ccgttggcga gcgcgttggc gcgcgcgcgc tcgacgagcg
      181 ccttcgcgcc ctccagcccg atcacctgcg cgccgcgcgc ggcgagcggc agcgagaagt
      241 ttcccaggcc gcagaacagg tcgccgatgc gctcgcccgg ctgcgggtcg agcagccgta
      301 cggcgcgcga tacgagcagg cgattgaccg catggttcac ctgcgtgaag tcggtgggat
      361 ggaaagcgat gcgcacgccg aactccggca gcgcgtaata cagcgccggc ggcgatccgg
      421 gatgcaaggg cgcggcactg tccggtccgc gcggctgcag ccagacctgc acgcgcgccg
      481 cgtcggcaaa cgtgcgcaac aggtcaaggt cggcctcgct gaacggctgc aggtggcgga
      541 acaccagcac cacggcttcg tcgcccaccg ccacctcgat ctgcggcacg cgctcccgga
      601 tcgacagctc gccaatgagc gcacgcagct tcgggatgag ggcggacacg tgtgacggca
      661 gcaccaggca gctccgcatg tcggcgacga acgtggagcg gcgctcgcga aagccgacca
      721 tcgccccgcc cttcttctcg acgtagcgca ccgagagccg cgcgcggcgg cggtagcccc
      781 actcctcgcc atagatcggc gggagcatcg attccggctt taccttgccg atacgctcga
      841 agcaatcttc cagccagcgc tgcttggcag ccacctgcgt gcacgtatcc gcatgctgga
      901 tggcgcagcc gccgcagttc tcgacccagg ggcatggcgg cgcgcggcgc ccgcttgccg
      961 ccttcagcac cgcgaccgtg cgcgcggtgt cgaacttggg cttggagcgc agcacctggg
     1021 catcgacggt ctcgccgccg agcgcgccct cgacgaacac gaccttgccc gcggcgttgc
     1081 gcgcgatgcc gtggccgccg gcatcgagcg cgtagacgtc taaccggacc aacgggtgag
     1141 gaattcgcgc cagtgcgggg ccttcagctt ctccagcgcg ccgcgcagca gcgcgcattc
     1201 ctttaggtac tgcggcccgg tgagcgcgcc gcgcagcttc tggaagcgca ggtaaaggag
     1261 gtaggtgttg gcggtatcgg cttcgcagta gtcgcggatc tcggccatgc gcccttcgag
     1321 gtaggccgcc cagacctgcg cgccgccgac gccgatcttg cccggcagcc ccgcgagctg
     1381 cgccacctcg tcgagcggcg cgttgttgcg cggctggtac atggccagca cgtccatcag
     1441 gtcgaggtgg cgcgcatggt aacggccgag gtagctgttg aagcggaagt cgcggtcgtc
     1501 ctcgccctgg tcccagaact tcggcgcggc gacgccgtgc accagcgcgc ggtagttgag
     1561 caccggcagg tcgaagccgc cgccgttcca ggacacgagc tgcggcacca gcttctcgat
     1621 gccgtcgaag aagcgctgga tagcctcgtt ctcgggcgtt tccggctcgc cgatggaaaa
     1681 caccctgatg ccctcgccct cccggagcac gcacgagatc accgcgacgc ggtgcagctg
     1741 cggcccgagg aagtcgctgc cggtctgggt gcgccgcttc tggaatgcga tttccgcgac
     1801 ttcccggtcc ggcagcgatt cgggcagcgc ataaaggcgt cgaatgccgc cgacatccgg
     1861 cacggtttca atgtcgaagg cgagaatcgg cgtcacgtgc aataaatgag gtctgtcccc
     1921 atttaatcgg ggaacacacc ggtggacagg tagcggtcgc cgcggtcgca gatgatggtg
     1981 acgatgaccg cgttcttcag ctccgcggca atgcgtagcg cgactgccgt cgcgccgccc
     2041 gaggaaatgc cggcaaagat gccttcctcg cgcgccaggc gccgcgtcat ctcttcggcg
     2101 tcggcctggc tcacgtactc cagccggtcg acgcgcgcct tgtcgtagat ccggggcagg
     2161 taggcttccg gccacttgcg gatgccgggg atctgcgcgc cctcctcggg ctggcagccg
     2221 acgatctgga cgttcgggtt cttctccttg aaaaagcgcg agcagcccat gatggtgccg
     2281 gtggtgccca tcgaggacac gaagtgcgtg atcttgccgc ccgtgtcacg ccagatctcg
     2341 ggtcctgtcg tctcgtagtg cgcgagcggg ttgtccgggt tggcgaactg gtcgagcatc
     2401 acccccttgc cctcgcgcac catcttctcg gcgaggtcgc gcgcggcctc catgccgccc
     2461 ttttgcggcg tgaggatgat ctcggcgcca aaggcgcgca tggtctggcg ccgctccacc
     2521 gacaggtgct cgggcatgat caggatcatg cggaagccgc ggatcgctgc cgccatggcg
     2581 agcgcgatgc cggtattgcc cgaggtgggc tcgatcagca cgtcgccctt cttgatcgcg
     2641 ccgcgctcct cggcgcggcg caccatggaa atcgcgggcc ggtccttgac cgagccggcc
     2701 gggttgttgc cctcgagctt ggcgagaatc acgttgccgc ggcgcgcgct gtcctcgccg
     2761 ggcaggcgct gcagccgcac cagcggcgtc tcgccgatga agtcctcgac ggtgcgcttc
     2821 acgcctgcag ccgctcgttc acgtacgccg cgacgccgtc ggccacgtcc atgaactcac
     2881 cgtcgtagcc ggcctcgcgc agctgcgtca ggtccgcctc ggtgaagctc tggtagcgcg
     2941 cggcgagctg cggcgggaac ggcacgtact cgatcagctt ctgcgccgcg agctcggcgg
     3001 cgctcgcgcg cgtgccgcgc gcggcattga tcaccgcggc ggcgagctcg ttgaaggtct
     3061 gcgcgcgccc ggtgccgcag ttgaagatgc ccgagcggcc gcggtgctcg agaaagtgca
     3121 ggttcacccg caccacgtcg tcgacgtgga tgaagtcgcg gcgctgctcg ccgtcgccga
     3181 agccgcccga gccgacgaag agcttcacgc gcccctcggc ggcgagctgg tgccaggcgt
     3241 gaaaggcgac cgaggccatg cggcccttgt gcgcctcgtt cggcccgtag acgttgaagt
     3301 agcgcaggcc gacgatctgc gccgcgccct ccggcagcgt gcgccgcacg acctggtcga
     3361 agaggaactt ggagtagccg tagacgttga gcggcgcctc gcactcgcgc tcctcgcgaa
     3421 agccgttctt ccccgcgccg tacaccgacg ccgacgaggc gtataccagc gccacttcct
     3481 cctcctggca ccagtcgagc agcgcgcgcg aatagcggta gttgttgtcc atcacgtagc
     3541 ggccgtcgga cgccatggtg tcggagcagg cgccctggtg cagcaccgct tccaccgcgc
     3601 cgtcgaactg gccggcctcc agccgcgcga ggaactcgcg cttgtcgagg tagtcctcga
     3661 tctcgcagcc gacgaggttg ggcaccttgt cgacgttctc caggttgtcg accgccagga
     3721 tctcggtaac gccgcgctgg ttgagcgcgg cgaccagccg cgacccgatg aacccggcgg
     3781 cgccggtaat gacgtgcagc atcagctccc cccgaacagc tcttcgcgcg tcaccaccgc
     3841 ggtgccgaac ttggtgacct tgatgccgcc ggcgcgattg gcgaggcgcg ccgcggactc
     3901 catggattcg cccgcggcca gcatcacggc gagcacgccg atcacggtgt cgccggcgcc
     3961 ggtgacatcc gcgacatcgc gcggctcgcc cttgatgcgc accgtgcgcc cgggccggaa
     4021 cagcgtcatg ccgccctcgc cacgcgtgag cagcagcgca tccagccgca gccgcgcgcg
     4081 cagcgcctgc gcgcgcgccg cgaggtcgcg ctcgtccttc cagcgcccga ccacgtcctc
     4141 gagctccttc aggttcgggg tgatcatgct cgcgccgcgg tagcgcgagt agtcgtcgcc
     4201 cttggggtcg accagcacgg tcttcttcgc gcggcgcccc tcgcggatca tggtggcgac
     4261 gtgcgcgagc ccgcccttgc cgtagtccga gagcagcacc acgtcgcacg cgggcagcgc
     4321 cttgcggaag tcagcaagct tcgaagccag cacctccttc gacggcggcg tctccatgtc
     4381 gatgcgcagg agctgctgct tcttgtgcac cacgcgcagc ttctgcgtgg tgcgcaccga
     4441 agggtcgcgc cgcaggctcg ccgtgacgcc ctcggcctcg agcagccggg ccacgcgctc
     4501 gccggcctcg tcgcgtccgg tcaccgacag cagcgtcgtc ggcgcgccga gcgcggcgca
     4561 gttgcgcgcc acgttcgccg cgccgcccag gcgctcctcc tcgcgctgcc acagcaccac
     4621 cggcaccggc gcctcgggcg agatgcgcgt ggcgtcgccg taccagtagc ggtcgagcat
     4681 cacgtcgccc accaccagca cgcgcgcctt gtccgtcgcc ggccgcctca tgtgcgccgt
     4741 ccgatggcgt agtactcgaa gccgtacccc tggaccacgg ccggatcgta caggttgctg
     4801 ccgcccacca ggccgacgta gccgctgccg atgacggtca cgttcatggc gagaggtcga
     4861 actcctcggt gcggcgcggc ggatagctct cccagctgcc gcaggccggg cagtgccagt
     4921 ggaattgtcg tgccttgaag ccgcactcct cgcagcggta gcgcgccaag cgccgggtat
     4981 gactatggac caggtttctg atcagctcga gatcacggcg ccgctcggga tgcgtgtcgg
     5041 cgacgatctg cgcttccagc aggcggtcga ggccgagcag ggtcggattg cggcgcaact
     5101 cgtcacggac gagcttgtag gcggcctccg ctcccttcgc ctcgagggtg gtctggaaca
     5161 cggtgtcgag caggtcgagc gacggatagc gctcgaggta gccggcgagc aacgtcagac
     5221 ccacttcgag ccgcgccacc tggcggtagg cgtcgagcag gcgcggcgcg accagcgcca
     5281 ggtaggccgg gttctgcgat tcgacccgct gccagtgctc gatggcgcgc tcgaggttgc
     5341 ccgcggcgcg ctccaggtcg ccgagcgaga tgctggcgcg taccgacttg cggttcgcct
     5401 ccagcgccgc ctcgaggtgc ttgcgcgctt cctgcggccg cgagtgcgtc gcctcgctcg
     5461 ccgccagctc gcacaagaac tgcgagatct gcttggcgcg cgactcgccc gagtcgtcct
     5521 gcagcttgcc ggccatgtcg atggcgcgcg cccagtcctt ctcctgctgg aagatctcga
     5581 gcaggtagcg gcgtgcctcg gcagcgtgcg cacctgtttc caggcgcgcg aacagctcct
     5641 cggcgcgatc gaggatgcct gccttcaggt agtcctgcgc cagctcgaac gccgcggcga
     5701 gcttctgctc ggcgccgagg tcggggcgct cgaccaggtt ctggtgcatg cggatggcgc
     5761 ggtcatactc gccgcggcgc cggaagaggc tgcccagcgc aaagtgcagc tcgacggtct
     5821 cgggatcgac cttgaccacc tcgatgaacg actcgatggc cttgtcgggc tgctcgttga
     5881 gcaggaagtt cagccccttg aagtacgagc gcggcagcgc gcgcgattcg gagaccaggt
     5941 gccggatgtc gatgcgtgcc gcgagccagc ccagtccgaa gaacagtggc agcgcgagca
     6001 gccaccagta ctcgaactcc atcagccctc cgccggcggc ggcggaagcg tcggcgcgtc
     6061 gtgggtgcgc gcgcccacgg cacgcagctc gcgccgcagg cgcgccgcct cgcgccgctg
     6121 gcgcagcacg gcgccgagcg ctgcgagcag gccgagcacg gcgccggcgg cgaagaaggc
     6181 gagcaacagc acgaccagag gtgcctgcca ggcgaggtcg aagtagaacc gcagggtgac
     6241 ggtttccgtg ttgcgcgccg cgaaggcgag cagcaggatg aacagcgcga tgcggatcgt
     6301 ccaggtgagc aagcgcatga ggtgagccgg ggtccgagcc ccgacgctac tgcgtggcct
     6361 gcgcctgctg ggcggcggcc tcgacagcgg agtcgacgcg ctcgcgcaac tccttgccgg
     6421 ccttgaagtg cggcacgtac ttctccggca cgtgcacctt ctcgcccgac ttgggattgc
     6481 ggccgatgcg cggcggccgg tagttgagcc cgaagctgcc gaagccccgg atctcgatgc
     6541 gatgccccga gagcagcgcc tgggtcatcg cgtcgaggat catcttcacc gcgtactccg
     6601 cgtctttcgc caccagctgc gggtagcgct gcgccagccg ggcgatcagt tcggacttgg
     6661 tcatggcggc cgtttcccct gccgttcttg gtccggtccg tttacttctg cggctcggaa
     6721 ctgcccttgt cgagcttggc ccgcagcagc gccccgagat tggtcgagcc cgaggcgctc
     6781 gcctggttct cgctgcgcag ctgcttcatg gcgtcggtct cttccgccat gtccttggcc
     6841 ttcaccgaca gattgatcga gcggttcttg cggtcgacgt tgatgatcat cgcgctcacg
     6901 ctgtcgcctt ccttgagcgc ggcgcgcagg tcgtcgatgc ggtcgcgcgc gaactcggag
     6961 gcgcgcaggt agccttcgat gccgtcggca agcgccacca cggcgccttt cgcgtccacc
     7021 gactttaccg tgcccggcac cacgctgttc ttgtcgtgcc cggcgatgaa attggtgaac
     7081 gggtcgccgc cgagctgctt gatgccgagc gaaatgcgct cgcgctcgac gtcgatcgcc
     7141 agcaccaccg cctcgacctc ctcgcctttc ttgaagttct tgaccgcttc ctcgccggcg
     7201 agcgaccacg acaggtcgga caggtgcacc aggccgtcga tgccgcccga caggccgacg
     7261 aacacgccga agtcggtgat cgacttgatc tgccccttga cgcggtcgcc cttcttgaac
     7321 tcgcgcgcaa aatcctccca gggattgggc ttgcactgct tcatgccgag ggagatgcgc
     7381 cggcgctcct cgtcgatctc gaggatcatc acctcgacct cgtcgccgac ctgcaccacc
     7441 ttggacgggt gcacgttctt gttggtccag tccatctccg agacgtgcac caggccttcg
     7501 atgcccgctt ccacctccac gaacgcgccg tagtcggtga ggttggtgac cttgccgaac
     7561 aggcgcgtgc cctgcggata gcggcgcgaa atgccgaccc agggatcctc gcccagctgc
     7621 ttcaggccaa gcgacacgcg gttcttctcg gcgtcgaatt tcagcacctt ggcggtgact
     7681 tcgtcgccga cgttgagcac ttcggaagga tgcttcactc ggcgccaggc gaggtcggtg
     7741 atgtgcagca gcccgtcgat accgcccagg tcgacgaatg cgccgtaatc ggtgatgttc
     7801 ttgacgatgc ccttcacgac cgcgccctcc tgcaggctgg agagcagcgc ctcgcgctcg
     7861 gcaccggcgc tcgcctcgag caccgcgcgg cgcgacacga ccacgttgtt gcgcttgcgg
     7921 tcgagcttga tgaccttgaa ctccatggtc ttgccttcgt acggcgtggt gtccttcacc
     7981 ggccgcaggt cgaccagcga ccccggcagg aaggcgcgga tccccttggt catgacggtg
     8041 agcccgccct tcaccttgcc ggtgatcacg ccctcgatct tctcgccctt ctccagcgac
     8101 tcctcgagct cgatccacgc ggccatgcgc ttggccttgt cgcgcgacag gcgcgtggcg
     8161 ccgtagccgt cttccagggc ctcgatcgac acggagacga aatcgccggg cttgacctcg
     8221 acttcgccgg cgtcgttctt gaactcctcg gcggcgatga tggattcgga cttcagcccg
     8281 gcgttgacga cgacatagtt gggatcgacg ctgaccacct cggcggtgat cacctcgccg
     8341 atgcgcattt cctttcgcgt caggctctct tcgaagagcg cggcgaagga ttcgttggcg
     8401 ggggacttgg agactgcgtg ggaaacgctt gccatgaaag ggaaaaacct cggtttcaac
     8461 cgctcggccg gcgaaggcgc gcgcgggtaa ggttgcaaaa aagggccggc cgtcaggccc
     8521 gcccacccag attcctctcc cggcaccacc ccaggatcag gccgacgact tcgtcgaggg
     8581 accggtccga ggagtccagc agcagcgcgt ccggggccgg cgccagaggc gccaccgctc
     8641 gggccacatc gcgggcatcg cgctgcgcca ggtcacggga aagggccgca aggttagcat
     8701 cgattccttt gtctatcaac tgcttatacc tgcgtcgggc ccgcacctcc ggggtggcgg
     8761 tgaggaacac tttgaggggc gcatccggga acaccacggt gcccatatcg cggccctcgg
     8821 ccaccagtcc gggcgcgcgc cggaaggctc gctggcgctc cagcagcgcc tgccgtactg
     8881 cgggccgttg ggcgacgtcg gacgcgcgta cgcccacccc ttcctcgcgc aaactttccg
     8941 taacgtcttg tccagaaagg aaaatccttc ctccccggaa ttccacttgc atgcggcggg
     9001 cggtgtcctc gggatgctca gggccagcca gtgcgaccag ccggtaaagg gccccgctct
     9061 caaggcaatg aaagccgagc gcctcggcca cctgctgcgc gaccgcgccc ttgcccgagg
     9121 ccgacggtcc gtcgatcgcg accaccggga ccgtcatcgg gcgatggcag cgaacgccgc
     9181 gaaatagccc ggaaaggtct tggcgacgca gtccgggtcg ttgatgcgca cgcgcacgcc
     9241 gccgagcgcg gcgagcgaca ggctcatcgc catgcggtgg tcgtcgtagg tgtcgaggct
     9301 cgcgccggaa acgagcctgg ctggcgccac cttgaggtaa tccaccccgg cctcgacgcc
     9361 ggcgccgagc ttggcgagct cggccgccat cgccgcgatg cggtcggtct ccttgacgcg
     9421 ccaactggcg atgttgcgca ccacgcaggg accgtcggcg aacagcgcca gcgccgccgc
     9481 ggtcatcgcc gcgtcgggaa tgtggttcag gtcgagatcg aacgcgcgca gcggcgcgcg
     9541 tccttcggcc tcgatccagt cctcgcccat cgacacctgc gcgcccatca cctcgagcac
     9601 ctcggtgaag cgcacgtcgc cctggatgct cgcgcgcccg acgccttcga cccgcaccgg
     9661 gccgccggcg atc
//
LOCUS       DBNHKE010000239         5805 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_416909, whole genome shotgun sequence.
ACCESSION   DBNHKE010000239 DBNHKE010000000
VERSION     DBNHKE010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_416909"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="AC16O9_16290"
     CDS             <1..1041
                     /locus_tag="AC16O9_16290"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6518216.1"
                     /translation="MCIGGTSQWPATIFRGIDQWGERYGYILVDPIGGAIGAFSHADG
                     ISTGGQARTPICKLPNVEHTEQSFPVLFLYRKEIVDSGGAGKFRGGLSAESCFIPHGT
                     DVIVQDTLSSGNAIPTSTGMMGGYPGAVNRYRFVRDSDALERMKRSALVGDIDELSGK
                     PETLQLRQENFVQNPADAYAVLWSAAGGYGDPLERDAKKVEADVENGDVSVEAAKSIY
                     GVVAGDAGATLAEREARRLARIGGTASGKKLNAAVTLQATENLAVRGGHWCCAKCATD
                     LGPVRDNYKMRCVRLDRPIQASNPIVGDPARFIDPTPQFRQFCCPGCGLLIENEVAVQ
                     SEPLLQDVELRG"
     gene            1062..2294
                     /locus_tag="AC16O9_16295"
     CDS             1062..2294
                     /locus_tag="AC16O9_16295"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF005734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HIR6518217.1"
                     /translation="MKKVTPVSGAGSSGNASSAAASVATTRMGDYTGILPIQKIREML
                     GSGEIKPLMRPIEPDQLQPASIDLRLGDYAYPIDASFLPGQGVRVLDKMKQLDGRFSD
                     FRIDLRNGAVLEKGRIYVVPLQEAIQLRSEVAAFANPKSSTGRLDILTRLIADGAAAF
                     DQVGEGFKGELYIEVAPRSFSVVVKTGTRLNQLRFRRTRGEEPKPITASDWNQLLEEG
                     KIVDVRDPERKTRSFKTGVLPFTVDLRGSAADNELLGYRARKHTRRIDLDRLDYDPLE
                     FWEPIRFHKTSSLILDPDEFYILMTREAIAVPPEYAAEMMPYDTRAGEFRVHYAGFFD
                     PGFGWDVRTGKAGSSRGVLEVRSHEIPFLLEHGQTVGWLRYERMAARPDVLYGAGSLS
                     NYQGQGLKLAKQFAPPAG"
     gene            2383..2841
                     /locus_tag="AC16O9_16300"
     CDS             2383..2841
                     /locus_tag="AC16O9_16300"
                     /inference="COORDINATES: protein motif:HMM:NF022479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HIR6518218.1"
                     /translation="MPSSLNDIAHVIQLAVAPVFLLTGVGALLAVLSGRLGRAVDRSR
                     VVEGQLPDLDQAALAGASEELALISRRTRLIYTAIALAVLCALLICVLIAVAFIDAFL
                     AINLARFLATLFVLAMLALIASLLAFLREIFLAVSSARTAMRRRAEGLKP"
     gene            2888..3394
                     /locus_tag="AC16O9_16305"
     CDS             2888..3394
                     /locus_tag="AC16O9_16305"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6518219.1"
                     /translation="MPVTELNHYLLVAKNLEKTKNFYQKVLGLKLASERPDFGFPGYW
                     LKAGQNICVHLASQAPNRIRDQFLLKKHRKGANGSGTVDHIAFLAKKPEEVRKRIQKH
                     RVAMHYRSFPESKLFQIFLKDPDDVTIELNFLGEVIDAKAWAGKGSASAVTLSKSDGR
                     RTSAARQN"
     gene            3397..3864
                     /locus_tag="AC16O9_16310"
     CDS             3397..3864
                     /locus_tag="AC16O9_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6518220.1"
                     /translation="MALERIDHYLLQTTDLAATRDWYVETLGMREGPHPDFKFPVVWL
                     YLGDKDVLHLSEGGRDVSENRKRYLGQESQAERGSGVIDHVAFAGTGLKQMIAHLTKL
                     GVPFQQRMADDQGLYQLFLKDPVNDVKVELSYANAEARTADLRPELTASQLRA"
     gene            complement(3854..4711)
                     /locus_tag="AC16O9_16315"
     CDS             complement(3854..4711)
                     /locus_tag="AC16O9_16315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6518221.1"
                     /translation="MDSRELGLVVAQQLLDVDDLHYGLWDGSLELTLANVPIAQQRYT
                     ELLLSALPGAAPGPVRVLDVGCGTGNLIAHLLERGYLADGVSPSPGLTRLARKRLERI
                     AGSTARIFECKFEDLPVDRLEHRYDVVLFGESFQYIELESALQLLPRLLKPGAQLVIC
                     DFFKTEHHGDGQPGDRTFGGGHAWRAFNERMRSAPFALERDEDITLRVSPNVELVNQV
                     LEQRLMPAAGTLGEYLASNYPKLAWVFGRLFRKKLAKLRRKYFSGHRTAATFERYKTY
                     HLLVYRFRP"
     gene            complement(4724..>5805)
                     /locus_tag="AC16O9_16320"
     CDS             complement(4724..>5805)
                     /locus_tag="AC16O9_16320"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6518222.1"
                     /translation="MPDATLRDLHDAGLFRMQQPRRVGGSEIEFAAVVTFGALVARAC
                     ASTAWNLINLGSHHLLLGMFPPKVQDEVWSASPDTLIASSFVFPAGRAQRVDGGYRIS
                     GRWPFSSGVDPSDWNMLAGQVAGAEGEPPEQRVFLLHKSQYTVHDTWFAGGLRGTGSK
                     DIEVKDQFVPEQRTLAVADMKGGPTPGSAVNPGPLYQMPVFAMFPYMLSGVALGVAEG
                     LIDDFAAGSAGRGKMTGAKISAVQSVQLRLGEAAALARASRLFQVGNCREAEGTIKAG
                     KVPDIQTKARYRLEGAYAVDWAVKAVDVLFMLSGASGLYESGHVARAFRDAHAVKQHF
                     SFNTDVAGTTYGRVALGLPSDNPTL"
BASE COUNT          993 a         1950 c         1978 g          884 t
ORIGIN      
        1 atgtgcatcg gcggcaccag ccagtggccg gcgaccatct tccgcggcat cgaccagtgg
       61 ggcgagcgct acggctacat cctggtggac ccgatcggcg gcgcgatcgg cgcgttctcc
      121 cacgccgacg gcatctccac cggcggccag gcgcgaacgc ccatctgcaa gctgcccaac
      181 gtcgagcaca ccgagcagag cttcccggtg ctgttcctgt accgcaagga aatcgtcgat
      241 tccggcgggg cgggcaagtt ccgcggcggc ctgtcggcgg agtcctgctt cattccgcac
      301 ggcaccgacg tcatcgtgca ggacaccctg tcgtcgggaa atgcgatccc gacgtcgacc
      361 ggcatgatgg gcggctaccc gggggcggtg aaccgctacc gtttcgtgcg cgactcggac
      421 gcgctggagc gcatgaagcg ctcggcgctg gtgggagaca tcgacgagct ctccggaaaa
      481 ccggagacgt tgcagctgcg ccaggagaac ttcgtgcaga acccggccga tgcctacgcc
      541 gtgctgtggt cggcggccgg cggatacggc gacccgctgg agcgcgacgc gaagaaagtc
      601 gaggccgatg tcgagaatgg cgacgtgtcg gtcgaggcgg cgaagtcgat ctacggggtg
      661 gtcgcgggcg acgccggcgc gacgctcgcc gagcgcgagg cgcggcggct ggcgcgcatc
      721 ggcggcacgg cctcgggcaa gaaattgaac gccgcggtga cgctgcaggc gaccgaaaac
      781 ctggcggtgc ggggcgggca ctggtgctgc gcaaagtgcg ccaccgatct cgggccggtg
      841 cgggacaact acaagatgcg ctgcgtgcgg ctcgaccgcc cgatccaggc cagcaacccg
      901 atcgtcggcg atccggcgcg cttcatcgac ccgacgccgc agttccggca gttctgctgc
      961 ccggggtgcg ggctgctgat cgagaacgag gtcgcggtgc aatccgagcc tttgctgcag
     1021 gacgtcgagc ttcgcggtta gacgacgccc atcgcgaccg cgtgaaaaag gtgactccgg
     1081 tgagtggggc gggctcgagc ggcaatgcga gttccgctgc ggcctccgtg gccacgaccc
     1141 ggatgggcga ttacaccggg atcctgccca tccagaagat aagagaaatg ctgggcagcg
     1201 gcgagatcaa gcccctcatg cggcccattg aacctgacca gctgcaaccg gcaagcatcg
     1261 atctgcggct cggcgattat gcttacccca tagacgccag ctttctcccg ggccagggcg
     1321 tcagggttct ggacaagatg aagcagcttg acgggcgctt cagcgacttc cgcatcgact
     1381 tgcgcaacgg cgcggtgctc gaaaagggca ggatctacgt cgtccctctc caggaagcga
     1441 tccaattacg aagcgaggtc gcggcgtttg ccaatccgaa gagctcgacg ggtcggctag
     1501 acatcctgac gcgcctcatc gccgatgggg ccgcggcatt cgaccaggtc ggcgagggat
     1561 tcaaggggga gctctacata gaagtcgcgc cgcgaagctt cagcgtggtg gtcaagacgg
     1621 gaacgcggct caatcagctc aggttccggc gcacccgagg cgaggagccc aaacccatca
     1681 ccgcgtcgga ctggaatcag ctgctggagg aaggcaagat cgtcgatgtg cgagacccgg
     1741 agcgaaagac aaggtcgttc aagacgggcg tactgccttt caccgtggat ctgcgggggt
     1801 ccgccgcgga caatgagttg ctcgggtacc gggcaaggaa gcacacgagg cgcatcgatc
     1861 tggacaggct cgactacgat ccgctcgagt tctgggagcc gatcaggttc cacaagacat
     1921 cgtcgctcat tcttgatcct gacgagttct acatcctgat gacgcgagag gcgatcgccg
     1981 tgcccccgga gtacgcggcg gagatgatgc cctacgacac gcgcgccggc gagttcaggg
     2041 tccattacgc gggcttcttc gatccggggt ttggatggga tgtgcggacc ggcaaagccg
     2101 gcagcagcag aggcgtgctc gaggtccgct cccacgagat tccgttcttg ctggagcacg
     2161 ggcaaaccgt cggctggctt cgctatgaac gcatggcggc ccggcccgat gtgctctacg
     2221 gcgccgggtc cttgtcgaac taccaggggc aaggcttgaa gctggccaag cagttcgccc
     2281 cgccggcggg ctaggcgtgc acctcggcac gctgccgcgg cacgagcggc gcgacggctg
     2341 cccgtccgcc gcctggaatt gcgcgcaccc gacgaggtag ccatgccctc cagcctcaac
     2401 gacatcgccc acgtcatcca gctcgcggtc gcgcccgtgt tcctgctcac cggcgtcggc
     2461 gcgctgctcg ccgtgctcag cgggcgcctc ggccgggcgg tggaccgcag ccgcgtggtg
     2521 gaagggcagc tgcccgacct cgatcaagcg gcgctcgccg gggcgagcga agagctcgcg
     2581 ctgatctcgc gccgcacgcg gctcatctac accgccatcg cgctcgccgt gctgtgcgcg
     2641 ctgctcatct gcgtgctgat cgcggtggcc ttcatcgacg ccttcctggc gatcaacctc
     2701 gccaggttcc tcgccacgct gttcgtgctg gcgatgctcg cgctgatcgc cagcctgctc
     2761 gcgttcctgc gcgagatctt cctcgccgtc agcagcgcgc gcaccgcgat gcgccgccgc
     2821 gccgagggat tgaagccctg agccgacccg tggacaatac ggtccgagtc accgaggagc
     2881 ccgccgcatg cccgtcaccg agctgaacca ctacctcctg gtcgccaaga acctggagaa
     2941 gacgaagaac ttctaccaga aggtgctggg cctcaagctc gcgagcgagc gcccggattt
     3001 cggcttcccc ggctactggc tgaaggcggg ccagaacatc tgcgtgcacc tcgcctccca
     3061 ggcgccgaac cggatccgcg accagttcct gctgaaaaag cacaggaagg gcgccaatgg
     3121 ctcgggcacc gtcgaccaca tcgcgtttct cgcaaagaag ccggaggagg tcaggaagcg
     3181 catccagaag cacagggtgg cgatgcacta ccgcagcttt ccggagtcga agctgttcca
     3241 gatcttcctc aaggaccccg acgacgtcac catcgagctc aacttcctcg gcgaggtgat
     3301 cgatgcgaag gcctgggcgg gcaagggctc ggcgtcggcg gtgacgctgt ccaagtccga
     3361 cggcaggcgc acctcggcgg cccgccagaa ctagcgatgg cgctcgagcg catcgaccat
     3421 tacctcctgc agaccacgga cctcgcggcg acgcgcgact ggtacgtcga gacgctgggc
     3481 atgcgcgaag gcccgcatcc ggacttcaag ttcccggtgg tgtggctcta cctcggcgac
     3541 aaggacgtgc tccacctgag cgagggcggg agggacgtgt ccgagaaccg caagcgctac
     3601 ctcggccagg aatcgcaggc ggagcggggc tcgggcgtca tcgaccacgt cgccttcgcc
     3661 ggcacgggcc tcaagcagat gatcgcgcac ctgacaaagc tcggcgtgcc gttccagcag
     3721 cgcatggcgg acgatcaggg cctgtaccag ctcttcctca aggacccggt caacgacgtg
     3781 aaggtcgagc tttcctacgc caacgccgag gcgcggaccg cggacctccg gcccgagctc
     3841 acggcgtcgc agcttagggc ctgaagcggt agacgaggag gtggtaggtc ttgtagcgct
     3901 cgaaggtcgc cgccgtgcga tggccggaga agtacttgcg gcgcagcttg gccagcttct
     3961 tgcggaacag ccgcccgaaa acccaggcaa gcttcggata gttgctggcc aagtactcgc
     4021 ccagcgtccc ggcggccggc attagccttt gttccagcac ctgattcacc agctcgacgt
     4081 tgggactgac gcggagcgtg atgtcctcgt cgcgctcgag cgcaaagggc gcgctgcgca
     4141 tgcgctcgtt gaatgcgcgc caggcgtggc cgccgccgaa ggtgcggtcg cccggttggc
     4201 cgtcgccatg gtgctcggtc ttgaagaaat cgcagatcac cagttgcgct ccgggcttga
     4261 gcagccgcgg cagaagctgc aacgcgctct ccagctcgat gtactggaag ctttcgccga
     4321 acagcaccac gtcgtagcgg tgctcgaggc gatccaccgg caggtcctcg aacttgcatt
     4381 cgaagatccg tgccgtgctg ccggcgatgc gctccagccg cttgcgtgcg agccgggtga
     4441 gcccgggcga cgggctcacg ccgtcggcga ggtagccgcg ctccagcagg tgggcgatca
     4501 ggttgccggt gccgcagccc acgtcgagca cccgcacggg gccgggagcg gcgcccggaa
     4561 gcgcggaaag cagcagctcc gtgtagcgct gctgggcgat gggcacgttg gcgagcgtga
     4621 gctcgagcga cccgtcccag agcccatagt gcagatcgtc gacatccagc agctgctgcg
     4681 cgacgaccaa ccccagctcg cggctatcca tgctgcggct cggctagagc gtcggattgt
     4741 cggaaggcag cccgagcgcc acgcgcccgt aggtggtgcc ggcgacgtcg gtattgaacg
     4801 agaagtgctg cttcaccgca tgcgcgtcgc ggaacgcgcg cgccacgtgc cccgactcgt
     4861 acaggccgct cgctcccgag agcatgaaca gcacgtccac cgccttcacc gcccagtcca
     4921 ccgcgtaggc gccctcgagc cggtagcgcg ccttggtctg gatgtcgggc accttgcccg
     4981 ccttgatcgt gccttccgcc tcgcggcagt tgccgacctg gaacaggcgc gaggcgcgcg
     5041 ccagcgcggc ggcttcgccc aggcgcagct gcacgctctg caccgcggaa atcttggcgc
     5101 cggtcatctt gccgcgcccc gccgagccgg cggcgaaatc gtcgatcagc ccctcggcga
     5161 cgccgagcgc gacgcccgag agcatgtacg ggaacatcgc gaacaccggc atctggtaga
     5221 gcggccccgg gttcaccgcg ctgccgggcg tcgggccgcc cttcatgtcg gccaccgcga
     5281 gcgtgcgctg ctcgggcacg aactggtcct tcacctcgat gtccttcgag cccgtgccgc
     5341 gcaggccgcc ggcgaaccag gtgtcgtgca ccgtgtactg ggacttgtgc agcaggaata
     5401 cgcgctgctc gggcggctcg ccctccgccc ccgccacctg cccggcgagc atgttccagt
     5461 cgctcggatc gacgccgctc gagaacggcc agcggccgga gatgcggtag ccgccgtcga
     5521 cgcgctgcgc gcgacccgcg ggaaagacga acgaggaagc gatcagcgtg tcgggcgaag
     5581 cgctccagac ctcgtcctgc accttgggcg ggaacatgcc gagcagcagg tggtgcgagc
     5641 cgaggttgat caggttccag gccgtggacg cgcaggcgcg cgccaccagc gcgccgaagg
     5701 tgaccaccgc cgcgaactcg atctcggaac cgccgacgcg gcgcggctgc tgcatgcgga
     5761 acagccccgc gtcgtgcagg tcgcgcagcg tcgcgtcggg catgc
//
LOCUS       DBNHKE010000240        23364 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_311744, whole genome shotgun sequence.
ACCESSION   DBNHKE010000240 DBNHKE010000000
VERSION     DBNHKE010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 23364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_311744"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC16O9_16325"
     CDS             complement(<1..434)
                     /locus_tag="AC16O9_16325"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6518223.1"
                     /translation="MSELNSNPAEAPSAAVEEPVGSVLARAREAAGLPLEEAARRLKF
                     APRQLDALERGEWGRLPGGAAVRGMVRSYARLLGLDADALVARVNSGQALPDAERLAE
                     RFRQPVPFSDGSRRSNLAYVVLSVALLAVVAAVVLEWRNERAG"
     gene            complement(427..1200)
                     /gene="pilW"
                     /locus_tag="AC16O9_16330"
     CDS             complement(427..1200)
                     /gene="pilW"
                     /locus_tag="AC16O9_16330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HIR6518224.1"
                     /translation="MRRLLACALVALAGCAAQPTGPVGNTGTIVGEPSAPRNRARIHT
                     ELATMYYARGNMSVALEELRTAVAADPTYATAHGVFGLVYKELRENTLAEESFRRALG
                     HAPGDPDINHNYGWFLCQIGRESEAKPYFRRALDNPLYATPARTFAAAGICALRSGER
                     AVAEENLQRALRLDPNLPIALLQFAQLRYTEGRYDEARRLLTQHAAVAQQSAESLWLA
                     LRVERRLGARAAEQSYAIQLRRRFPNSPEFQQLQRGSYE"
     gene            complement(1197..2273)
                     /gene="rlmN"
                     /locus_tag="AC16O9_16335"
     CDS             complement(1197..2273)
                     /gene="rlmN"
                     /locus_tag="AC16O9_16335"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR6518225.1"
                     /translation="MKANLLDLDAAALAAFFAEQGEKPFRARQVLRWVHQSGESDFER
                     MSDLAKALRAKLAASARVEAPRVVGDSTAADGTRKWLLKVDGANAVDAVFIPETGRGT
                     LCVSSQAGCVLDCAFCSTGKQGFNRNLSTAEIVGQLWLANRLLEGERPVTNVVLMGMG
                     EPLLNLENVIPALRLMLDDNAYGLSRRRVTVSTSGVIPGMDQLRDACPVALAVSLHAP
                     SDELRDRLVPVNRKYPIRELLKACRRYLEKAPRDFITFEYVMLEGVNDGEAEARALAR
                     LAAQVSCKFNLIPFNPFPRTEFRRSPPERIRRFAQVLQAKGATVTTRKTRGDAIDAAC
                     GQLAGEVADRTRRSRALRVAEAQP"
     gene            complement(2279..2704)
                     /gene="ndk"
                     /locus_tag="AC16O9_16340"
     CDS             complement(2279..2704)
                     /gene="ndk"
                     /locus_tag="AC16O9_16340"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR6518226.1"
                     /translation="MALERTLSIIKPDAVKKNVIGKILARFEAAGLRVIAARMMHLSR
                     AEAEGFYAVHRERPFFRDLVDFMISGPVVVQVLEGDNAIARNRDLMGATDPKKAAKGT
                     IRADFADSIDANAVHGSDGPDTARVEIAYFFPACEVFSR"
     gene            complement(2790..3947)
                     /gene="tilS"
                     /locus_tag="AC16O9_16345"
     CDS             complement(2790..3947)
                     /gene="tilS"
                     /locus_tag="AC16O9_16345"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6518227.1"
                     /translation="MASSRKPPSADLGGLDLRGKRVAVGLSGGVDSVVLLDLLRTAAP
                     RHGFRVEALHVNHGLSPRAGEWARFCRSLCRKWGVPLTVRRVKVDKRGHGLEAAARAA
                     RRTVFARHPAPVIALAHQLDDQAETVLLNLLRGAGVRGASAMPTVGRVGDKTLLRPLL
                     AVRRREIEAYARSRGLRWVEDESNRDAALTRNFLRLRVGPLLATRFPRWRESLARAAR
                     HFASADDRARLALREFLRGHGLRAPSEAKLVDMLRQLTGKGTRTAIEHDGALLRVYRG
                     RVSIDRGARGELRLRPARGRGIAAARLERARLTLRARTGGERLQLDARRPRRTLKNLF
                     QEAGVPAWRRERLPLLYCDDELVWVPELGVAAGWQAARGARGLVPEWRETS"
     gene            complement(3916..4884)
                     /locus_tag="AC16O9_16350"
     CDS             complement(3916..4884)
                     /locus_tag="AC16O9_16350"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR6518228.1"
                     /translation="MKTTFLDFEQPIAELEQRIEELRFVQDDSAVDISDEIQRLAKKS
                     QALTKEIYAKLTPWQVAQVARHPQRPYTLDYVGTLFTGYEELHGDRSFAEDASIVGGL
                     ARFDGLPCVVIGHQKGRDTKEKILRNFGMPKPEGYRKALRLMKLGEKFELPVFTFVDT
                     PGAYPGIDAEERGQSEAIGRNLYAMAGLRTPIIATIIGEGGSGGALAIAVGDVTLMLQ
                     YATYSVISPEGCASILWKSADKAPEAAETLGITATRLKALGLVDKVVPEPLGGAHRDP
                     QAAAQSLKKALAEAWRDLRSTKPKQLVEDRLERLMSYGKFKEAAER"
     gene            complement(4899..6002)
                     /locus_tag="AC16O9_16355"
     CDS             complement(4899..6002)
                     /locus_tag="AC16O9_16355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6518229.1"
                     /translation="MSEGTLASSAALGDEQARQSLRLRRYLMGAGTSLLVAASLLLWH
                     WFGLLPLKIAIGGAALIGFFIVLFYGMFRSGLNQRFRDPSLTSEMIVAALMTLAYLMY
                     HAASARDALSLFYLVALLFGVLRLGTARLLVLALIALAAHAGVLFATGALQGQGARAN
                     WLQLAALAVVLPWFAVMGGYVNGLRLRLSESNRRLKEAADRAEEHAIRDVLTGVHNRR
                     HLLDMLQREIARAARLGRPLSVCMIDIDHFKSINDTLGHAAGDTVLRHFAVLASSGLR
                     AMDVFGRFGGEEFMLILPDTEASGAAATAERLRSSVERREFPGLPAQRRVTVTIGIAA
                     HLKGESGEALTARADGALYRGKHAGRNQVVVLR"
     gene            complement(6004..6552)
                     /locus_tag="AC16O9_16360"
     CDS             complement(6004..6552)
                     /locus_tag="AC16O9_16360"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR6518230.1"
                     /translation="MRACPRVHLVRRGEPFMTLARAIVGQQISVKAAQSVWDRVIACV
                     GDCTPQAVLRREPRSLRACGLSDRKTEYIVDLARRFDRGLVHPHRWPALSDEEIIADL
                     VQVRGIGRWTAEMFLIFNLLRPDVFPLDDLGLQAALRRHYFGGRRTSPARMRRLGEQW
                     APWRSVATWYLWRSLDPLPVEY"
     gene            complement(6611..7639)
                     /locus_tag="AC16O9_16365"
     CDS             complement(6611..7639)
                     /locus_tag="AC16O9_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HIR6518231.1"
                     /translation="MRGWSRRLPDFWRNSGFHLLERDPAGRLRVTDDFLRAYYLRPEI
                     HPVEESCDAELRLHASLMETPRRAVAAAEIDALADQDARHNYRMLLRFRDRLLAAGTL
                     EGCYMTLFKGDIDVPPLFIEQLAHVILRNILDGAEDPLRLRAAELFFRDQKATLQDGH
                     VLLADHETVEMHAAGNRYGSIGKLIVEAQGTLGKVDLDVLDRANAALYWERESRHDTV
                     ISLTYGRPALEALCRVIEGWIAHFHAVEVRVKPLRKIDEARWAWHIGLDAESTDILNE
                     LWSGSQVDAGRMRNIVALFSMQFADPALMRESVSGRTVYLALSVNEAQVVRMKPQNLL
                     LNLPLREA"
     gene            complement(7611..8189)
                     /locus_tag="AC16O9_16370"
     CDS             complement(7611..8189)
                     /locus_tag="AC16O9_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR6518232.1"
                     /translation="MDNLLILSLDSHGVPHRWISWQQACFYYAKNLVAWTLGERTFTF
                     RGGMSRLTGERSSITAHSIIAIRGRALAARGFHQVPPLNNRELFRRDRHTCAYCGHEY
                     GYFRLTRDHITPLSRGGRDTWMNVVTACRHCNGHKRNRMPQECGMELMYAPYVPNKAE
                     YLILTNRRIFADQMEFLRQHVAAHSRLVASPA"
     gene            complement(8257..8544)
                     /locus_tag="AC16O9_16375"
     CDS             complement(8257..8544)
                     /locus_tag="AC16O9_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6518233.1"
                     /translation="MRSVTKADLVEAAARAGNLTKSSAGEAVNAVFDAIVASVAKGNR
                     VTVVGFGTFLPRKRKARAGRSPLNGKEIRISSKTVPAFAAGQGFKDRVASR"
     gene            complement(8664..9548)
                     /locus_tag="AC16O9_16380"
     CDS             complement(8664..9548)
                     /locus_tag="AC16O9_16380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6518234.1"
                     /translation="MSEQPRLGYIGIGLMGRPMSLRLLAAGYALTVWNRSRDKLVPLT
                     AKGAQAVDSPAAVARAADIVMMCVTDQHAAGQVLFGPGGIAAGGAPGKLLVDFSSLAP
                     SSAREFATRLERECGMGLVDAPVSGGTVGAEQGTLAIMAGGKPEHIERARPVLAHLAQ
                     RFTRMGDAGAGQVTKLCNQVIVGCLFPVIAEAVRLAEAAGVDAHALPQALKGGFADSL
                     PLQVFGARMAARQFDPPLGAVATMLKDLENAAEVAKGARVPLPMARTAAELYRLLVAQ
                     GRGEHEPSALVDLLAGKR"
     gene            complement(9545..11227)
                     /gene="ilvD"
                     /locus_tag="AC16O9_16385"
     CDS             complement(9545..11227)
                     /gene="ilvD"
                     /locus_tag="AC16O9_16385"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6518235.1"
                     /translation="MARRSIPILPSRVTVEGLDRAPHRAFLRSLGVGDADLGKPFVGV
                     VSTDGRVTPCNALLGEFARTACDGVREAGGVPFDFASVSVADSMSMNHGGMRYSLVSR
                     ELIADGIEAVARGHAYDGLVAFAGCDKTLPGMMMALVRLDLPGVFVYGGAALPGRWHG
                     RDVTVLDTYEGVGAVLAGDMPASDLDELERACLPTLGSCPGQFTANTMAMVSETLGLA
                     PPGTATLPAVDAARGAAARAAGAAVMRLLRDGGPRPRELVTRKSLENACAAVAATGGS
                     TNAALHIPAIAHEAGIRFDMDDFARVAKRTPLIADMKPGGRYLAKDLHAAGGVYAVLK
                     ALLERGALHGGCLTLSGSTLEAALADHAGADGDVVRKAPILKTGGLVVLKGNLAPEGA
                     LIKVAGLKTLVFEGPARVFDSEEACADVVRRGRYRPGDVLVIRYEGPKGGPGMREMLG
                     VTALIYGQGAGEKVALLTDGRFSGATRGMMVGYVSPEAAAGGALALVRNGDRIRIDAA
                     AGELTLLVPRVELRRRKMKAPRRAPLSGVLEKYAAVVGSAHRGAVTHSGGRK"
     gene            complement(11230..12204)
                     /locus_tag="AC16O9_16390"
     CDS             complement(11230..12204)
                     /locus_tag="AC16O9_16390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR6518236.1"
                     /translation="MRLVTRGLLAACAALSIAPAAAQQPTYPTKSIRFVVAFAPGEPG
                     DVLARAMVPLLAERLRQPVVIENKPGGNGSEGIDLVAKAAPDGYTIGLGGSGALATNS
                     SLYAKMPYNPVTDLAPVTSIAQFPFLLVAPPTLPAATLEELLALARAKPGEMLIGFGG
                     NGTATHLAAELFKLMAKVQMVNVPYKGDGAAAADAANGKLSLALVDVQSALPHVKSGK
                     LKALAVSSAKRVFAAPDAPTFAEAGLPGYEAGDWLGIVVPVTTPGAIVARLNGDFVAA
                     LKRAEVRDRIIAAGAEPAPSTPVGFGAVIAAEVVKWSEVVQLSGARGD"
     gene            complement(12274..12921)
                     /locus_tag="AC16O9_16395"
     CDS             complement(12274..12921)
                     /locus_tag="AC16O9_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518237.1"
                     /translation="MQLARKPTEIFSPASGALDKAPAETWRSYEPIFRAHATAVITPD
                     FLAALAQVEGAGNPLARTPWRWRLTEDPFDVYRPASSAVGMYQITDGTLQQAKRYCIH
                     DNQVAEDGPWHDWRSCWFNGLYFRVVPSHAAEMTAAFLDKSVSRTLEARKIDATLRQK
                     QDLAAIMHLCGVAAGEAFAKRGFQLGSNQRCGGQNVGGYLGQVHALQRTFATLGR"
     gene            complement(13047..13178)
                     /locus_tag="AC16O9_16400"
     CDS             complement(13047..13178)
                     /locus_tag="AC16O9_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518238.1"
                     /translation="MKILPALLLVTLIGASACAAATRQERQRVVKNCLRNRGYSVLN"
     gene            complement(13199..14173)
                     /locus_tag="AC16O9_16405"
     CDS             complement(13199..14173)
                     /locus_tag="AC16O9_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6518239.1"
                     /translation="MLRVAICGAGNWGTRLIESVQGVSPKIRFVAAVTRDPAALKPLG
                     ERFGLALTSRYADVLADSAIDAVVLATPHSQHAAEIAAAAQARKHVFVEKPFTLTRES
                     AERAIEACRAAGITLHVGFNRRYAPAYAEMKRRIAAGEIGALRHIEGNFSGPPSYQIA
                     PGNWRSNRIESPGGSMTARGCHVLDAMVHLAGLATEVLAISERQQLDIDVDDTTSCLL
                     RFAGGATGTLATLHAATRFYRLHAFGAKGALELRGDTALELSDLEGNVRRFAFAATDK
                     ERAELEAFADAAAGGLKFVIAPEEIVNVVAGTEAVVASARSGNAIKIG"
     gene            complement(14178..15167)
                     /locus_tag="AC16O9_16410"
     CDS             complement(14178..15167)
                     /locus_tag="AC16O9_16410"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6518240.1"
                     /translation="MLAAHVGAFGRPEAAIEVLEQPDPGAPGPEEVVVETEFFPINPA
                     DVLNIEGKYGAVPPALPMTPGAEGVGRIAAAGAAVRHVKPGDRVLLPGPGCWRERIKA
                     PAKAVFALPEGVDPKQLAMLRVNPPTAYLMLHQFVAPKPGQWVIQNAANSGVGHCLIR
                     LARQAGVKTVNVVRREELIKPLQAFGGNVVLTDGPDLDKRVRAAIGEAPLPLAIDAVG
                     GSATQRLARCVSDGGTVVNYGVLSGEPCMVDGRDTVFRDVHLRGFWLRKWYVDTPPAQ
                     VAALYRELTSWVADGTLAVEVEAVYPIGKIKEAVAHAARGGRSGKILVSFAKG"
     gene            complement(15339..15686)
                     /locus_tag="AC16O9_16415"
     CDS             complement(15339..15686)
                     /locus_tag="AC16O9_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6518241.1"
                     /translation="MTEGQSTVSDIRLDHMNIPARDPLGLAQWYARTFSLKAEEHLVR
                     GPGLLIAFHSGAPVNRSPDELHMGFRVPSLEALSEWAKKFDATPKAGPEFTSFRITDP
                     EGNGIELYTPSRA"
     gene            complement(15762..16955)
                     /locus_tag="AC16O9_16420"
     CDS             complement(15762..16955)
                     /locus_tag="AC16O9_16420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518242.1"
                     /translation="MRWLMLLILFLVRLAMGFQFQSVASTSAQLIDAFGLSYAQVGTL
                     IGLFLLPGVFIAIPSGALTRAISDKNLLMLGAVTMTVGAYVMGSAADATELYAGRLIT
                     GVGGTIFNVILTKMVTEWFFEKEIVPALAIMLTAWPIGIALGLLIHNPVAAAYGWNSV
                     MYVTALAALAALLLTALFYRAPPAPLDLHGEPLRYGLPMRQLVHTSVVGVAWTLYNAA
                     FILVVSFMPGALISLGYERGAASSVTSLFMWTMMVSVPIGGRLLQSTRHVTLALAALL
                     LASSAMLVVITTGTLPEAGLVLVGIISGIPAGALMSLSAEAVSRENRGPGLGIFYTWY
                     YLGMTLMPVLAGWLRDWTGSAKAPVLLAALLMASVVACVALLRTLQARWRIDERGSGV
                     SAAAD"
     gene            17236..17577
                     /locus_tag="AC16O9_16425"
     CDS             17236..17577
                     /locus_tag="AC16O9_16425"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6518243.1"
                     /translation="MALFMLMGKYSPEAIKTIMETGSDREAAARTALEAAGGKLLGFY
                     GMFGQDYNVAIVAEVPGNAEYIGAIAPALMAGTFTAFKSIPLYKASDVVKASQIAKKV
                     SAAYTPPTKAK"
     gene            complement(17708..17851)
                     /locus_tag="AC16O9_16430"
     CDS             complement(17708..17851)
                     /locus_tag="AC16O9_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518244.1"
                     /translation="MTSVIPASLQACGIDFPPPVSTSIWRRFATTCSGLYFFFAICLL
                     LPS"
     gene            17787..18338
                     /locus_tag="AC16O9_16435"
     CDS             17787..18338
                     /locus_tag="AC16O9_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6518245.1"
                     /translation="MLTGGGKSIPQACKEAGITDVTYYRWRREYNEVRPHSSRATGRR
                     RRRPVAREPSCSRSRRWCNKVTTVLLQRIGQVNELVVRATSRALPNARTAERANCGNP
                     SAHTRNRSSWPHNPLTIVPMPATLKAQLDSDRAADCASLPTLRRESLTEANPSAHTAR
                     TPTPNHAGPARRSCPRQRSTRAL"
     gene            18482..19897
                     /locus_tag="AC16O9_16440"
     CDS             18482..19897
                     /locus_tag="AC16O9_16440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6518246.1"
                     /translation="MFNRDRPKVMAALPELIRPLADLVDENGMLSGDDLASRKPGWFI
                     DKLRADAVLRPTATEQVAAILTYCNEHAIGVVPQGGMTGLVRATETEAGNIVLSLERM
                     NRIEHIDIVGRTARVEAGVILQALQEKLAGCDLAFPLDLGARGSCQLGGNASTNAGGL
                     RVVRYGMMRDLVLGIEAVLADGTVVSSMNEMLKNNTGYDLKQLFIGSEGTLGIITRLV
                     LRLYEAPKSRSTALVGVSRFEQVTGLLKHMDSALAGQLSAFEVMWNSFYAFNTRFLGD
                     APLGTNYPWYAVVESLGGSPVRDQAQFEAALESALEAGLVDDAIVAKSDADAKRLWRV
                     RESIESMMGRPVFVYDVSLPIVRMEAYIRELEKRLPRELPGCEYGTFGHLADGNLHVV
                     VLLDRNQQGGGLAELHVVSDRAVYEPLAAIGGSVSAEHGVGLEKKPYLPLSRSPAELA
                     LMKRLKQLLDPNGILNPGKIF"
     gene            complement(20220..20705)
                     /locus_tag="AC16O9_16445"
     CDS             complement(20220..20705)
                     /locus_tag="AC16O9_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518247.1"
                     /translation="MGATRRLADRYWISDANWSSLPMIPAGTRIRTKEYSEHHITVDV
                     DVMYTQEMHLGLDYGRSQSITDWARKMIVQDDPRSRIAGWPESARHAVSAGKVAIGMT
                     REQVIVSLGYPPAHETPSLDADRWKYWYGKFDTLQVVWDAQGRVRDVLADPRVRSAVM
                     P"
     gene            complement(20936..21011)
                     /locus_tag="AC16O9_16450"
     tRNA            complement(20936..21011)
                     /locus_tag="AC16O9_16450"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20975..20977),aa:Glu,seq:ttc)
     gene            complement(21035..21110)
                     /locus_tag="AC16O9_16455"
     tRNA            complement(21035..21110)
                     /locus_tag="AC16O9_16455"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21075..21077),aa:Ala,seq:ggc)
     gene            complement(21142..21531)
                     /locus_tag="AC16O9_16460"
     CDS             complement(21142..21531)
                     /locus_tag="AC16O9_16460"
                     /inference="COORDINATES: protein motif:HMM:NF023696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HIR6518248.1"
                     /translation="MRGIVLLLALVSAGAHAQRMEPGEWEFVSEIAMPGLPRPQQSGY
                     RACLTPEQARDPMYWGRGAQVPSDCRVATLKLGPDYTSWALECPASNMRGAGRAQISR
                     NSMSGELKLTGGVVTKTRGRRLGPCKP"
     gene            complement(21528..22859)
                     /gene="gltX"
                     /locus_tag="AC16O9_16465"
     CDS             complement(21528..22859)
                     /gene="gltX"
                     /locus_tag="AC16O9_16465"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR6518249.1"
                     /translation="MTVRTRFAPSPTGFLHIGGARTALFSRAYARRHGGAFVLRIEDT
                     DTERSTRESEAAILEGLQWLGLDWDEGPFFQMQRLPRYREVAEQLIAAGHAYRDYMPR
                     EALDALRAEQSARGEKPRYDRAIGQKYKGEGPAPVRFRTPQEGTVAWRDLVKGPIEFP
                     NAELDDLVLLRADGVPTYNFGVVVDDLDMRITHVIRGDDHVNNTPRQIHIFRALGSTL
                     PEFAHVPMILGADGERLSKRHGAVSVTQYRDDGFLPEALANYLARLGWSHGDDELFSM
                     AELEQWFDLGHVSHSPAKFDPEKLAWLNQQYMKQADEARLAALAAPFLGREPGPAVVA
                     LLKERAGTVKELAAQAEMFYREPGAQSVPAEAKPALAALQKELESLAWERKALGAAVS
                     SAAKAHNLKMPQLAMPLRRVLTGREQTPSIDAVLELLGREAVLARIGRALA"
     gene            22974..23048
                     /locus_tag="AC16O9_16470"
     tRNA            22974..23048
                     /locus_tag="AC16O9_16470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23006..23008,aa:Val,seq:cac)
     gene            23067..>23364
                     /locus_tag="AC16O9_16475"
     CDS             23067..>23364
                     /locus_tag="AC16O9_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6518250.1"
                     /translation="MPESGATRIRLPGKHEADPSLAHGAGLTAKQLQAVQDDAWSDRT
                     LWTEEEWLVVRYAEALAAVPQEVDDKLFAALKSRFSERQIVDLTMRLALCAAWNK"
BASE COUNT         3766 a         8181 c         7836 g         3581 t
ORIGIN      
        1 cctgcacgct cgttgcgcca ctcgagcacg acggcggcca ccaccgccag cagggcgacc
       61 gaaagcacga cataagcgag gttcgagcgc cgcgagccgt cggagaaggg caccggctgg
      121 cgaaaacgct ccgcgaggcg ctcggcatcg ggcagcgcct gccccgagtt gacccgcgcc
      181 accagcgcat cggcgtccag cccgagcagg cgcgcgtagc tgcgcaccat gccgcgcacg
      241 gcggcgccgc cgggcagccg cccccactcg ccgcgctcca gcgcgtcgag ctggcgcggc
      301 gcaaacttga ggcgacgcgc cgcctcctcc agcgggagtc cggccgcctc gcgcgcgcgc
      361 gccagcaccg agccgaccgg ctcctcgacc gccgcggacg gcgcttcggc agggttggaa
      421 ttcagctcac tcatagctgc cgcgctgcaa ttgctggaac tccggcgaat tggggaagcg
      481 ccgccgcagc tggatggcgt aggactgctc cgccgcgcgc gcgcccaggc gccgctccac
      541 gcgcagcgcc agccacagcg actcggcgct ctgttgcgcc actgcggcgt gctgcgtaag
      601 caggcggcgc gcctcgtcgt agcggccctc ggtgtagcgc agctgcgcga actgcagcaa
      661 cgcaatgggc agattgggat cgaggcgcag cgcgcgctgc aggttttcct cggccacggc
      721 gcgctcgccc gagcgcagcg cgcagatgcc cgccgccgcg aaggtgcgcg cgggtgtcgc
      781 atacagcgga ttgtccagcg cgcgccggaa gtacggcttg gcttcgctct cgcggccgat
      841 ctggcacagg aaccagccat agttgtggtt gatgtcggga tcgcccggtg cgtggccgag
      901 cgcgcgccgg aagctttcct cggcgagcgt gttctcgcgc agctccttgt acacgaggcc
      961 gaacacgccg tgcgcggtgg cataggtcgg atcggcggcc accgcggtgc gcagctcctc
     1021 cagggccacg ctcatgttgc cgcgcgcgta gtacatcgtg gcgagctcgg tgtggatgcg
     1081 cgcacgattg cgcggggccg acggctcgcc gacgatggtg ccggtgttgc ccaccggccc
     1141 cgtgggctgc gccgcgcagc cggcaagcgc gacaagcgcg caggcgagca ggcgcctcac
     1201 ggctgcgcct ccgccacgcg cagcgcgcgg ctgcgccgcg tgcggtcggc gacctcgccg
     1261 gcgagctgac cgcaggcggc gtcgatggca tcgccgcggg tcttgcgcgt ggtgaccgtc
     1321 gcgcccttcg cctgcagcac ctgcgcgaag cgccggatgc gctccggcgg cgagcggcgg
     1381 aattccgtgc gcgggaaggg gttgaacggg atcaggttga acttgcagct gacctgtgca
     1441 gccaggcgcg cgagcgcgcg cgcctcggcc tcgccgtcgt tgacgccctc gagcatcacg
     1501 tactcgaagg tgatgaagtc gcgcggcgcc ttttccaggt agcgccggca ggccttgagc
     1561 agctcgcgga tcgggtactt gcggttcacc ggcaccagcc ggtcgcgcag ctcgtcgctg
     1621 ggcgcgtgca gcgacaccgc gagcgccacc gggcaggcgt cgcgcaactg gtccatgccc
     1681 gggatcacgc ccgaggtgga cacggtgacc cggcggcgtg acagcccgta ggcgttgtcg
     1741 tcgagcatca ggcgcagcgc cggaatcacg ttctcgagat tgaggagcgg ctcgcccatg
     1801 cccatcagca cgacgttggt caccgggcgc tcgccttcca gcagacggtt cgcgagccac
     1861 agctggccga cgatctccgc cgtggacaga ttgcggttga agccctgctt gccggtcgag
     1921 caaaacgcgc agtcgagcac gcaccccgcc tggctcgaca cgcacaaggt gccgcgaccc
     1981 gtctcgggga tgaacaccgc gtcaacagcg ttcgccccat cgaccttcag cagccatttg
     2041 cgcgtgccgt ctgcggcggt gctgtcgccg acgacgcgcg gcgcctcgac gcgtgcgctc
     2101 gccgcgagct tcgcgcgcag cgccttggcc aggtcgctca tgcgctcgaa atcgctctcg
     2161 cccgactggt gcacccagcg cagcacctgg cgcgcccgga acggcttctc gccctgctcc
     2221 gcaaagaatg cggccagcgc ggcggcatcg aggtcgagga ggttggcctt catcgctctc
     2281 agcgcgagaa tacctcgcag gcgggaaaga aataggcgat ctcgacgcgc gcggtgtcgg
     2341 gcccatcgga gccgtgcacg gcgttggcgt cgatcgagtc cgcgaagtcg gcgcggatgg
     2401 tgcccttggc cgccttcttc ggatcggtgg cgcccatcag gtcgcggttg cgcgcgatgg
     2461 cgttgtcgcc ctcgagcacc tgcaccacca ccgggccgga gatcatgaag tcgaccaggt
     2521 cgcggaagaa cgggcgctcg cggtgcacgg cgtagaagcc ttcggcttcg gcgcgcgaca
     2581 ggtgcatcat gcgcgcggca atcacgcgca ggcccgctgc ctcgaagcgc gcaaggatct
     2641 tgccgatcac gttctttttc accgcgtcgg gcttgatgat ggacaaggtg cgctcgagcg
     2701 ccatattccc gtttcccccg gagttcccaa gaaattgtta gcccgcgatt ctatcacggg
     2761 cgcgtcgcga gtttcgtaaa aacaagggct taggaagtct cgcgccactc cgggacgagg
     2821 ccgcgcgcgc cacgcgccgc ctgccagccg gcggcgacac cgagctccgg cacccacacc
     2881 agctcatcgt cgcagtacag aagcggcagc cgctcgcgcc gccacgccgg cacgcccgcc
     2941 tcctgaaaca ggttcttcag cgtgcgccgt ggccgccgcg cgtcgagctg cagccgctcg
     3001 cccccggtgc gcgcgcgcag cgtgagccgc gcgcgctcca ggcgcgcggc ggcgatgccg
     3061 cgcccgcgcg caggccgcag gcggagctcg ccgcgcgcgc ccctgtcgat cgacacgcgg
     3121 ccgcggtaga cgcgcagcag cgcgccgtcg tgctcgatcg cagtgcgcgt gcccttgccg
     3181 gtcagctgcc tgagcatgtc gaccagcttc gcttcgctcg gcgcgcgcag gccatggccg
     3241 cgcaggaact cgcgcagcgc gaggcgcgcc ctgtcgtcgg cgctggcgaa gtggcgcgcc
     3301 gcccgcgcga ggctctcgcg ccagcgcgga aagcgcgtcg cgagcagcgg cccgacccgc
     3361 aggcgcagga agttgcgcgt cagcgccgcg tcccggttgg attcgtcctc gacccagcgc
     3421 agcccgcgcg agcgtgcgta cgcttcgatc tcgcggcgcc gcaccgcgag cagcgggcgc
     3481 aacagggtct tgtcaccgac ccggcccacc gtcggcatgg cgctcgcccc gcgcacgccg
     3541 gcgccgcgca gcaggttcag cagcaccgtc tcggcttgat cgtcgagctg gtgcgcaagc
     3601 gcgatgaccg gtgcgggatg gcgcgcgaac acggtgcggc gtgccgcgcg cgccgccgcc
     3661 tcgagcccgt ggccgcgctt gtcgaccttc acgcgccgca ccgtgagcgg cacgccccac
     3721 ttccggcaga gcgagcggca gaaacgcgcc cactcgccgg cgcgcgggct cagtccgtgg
     3781 ttgacatgca gcgcctcgac gcgaaagccg tggcgcggcg ctgccgtgcg cagcagatcg
     3841 agcagcacca ccgagtcgac acctcccgag agcccgaccg ccacgcgctt gccgcgcagg
     3901 tccagcccgc ccaggtcagc gctcggcggc ttccttgaac ttgccatagc tcatgagtcg
     3961 ctcgaggcgg tcctcgacga gctgcttggg cttggtgctg cgcaggtcgc gccaggcctc
     4021 ggccagcgcc ttcttcagcg actgcgccgc tgcctgcggg tcgcgatgcg caccgcccag
     4081 cggctcgggc accaccttgt ccaccagccc gagcgccttc aggcgggtgg cggtgatgcc
     4141 gagcgtctcg gcggcctccg gcgccttgtc ggcgctcttc cacaggatcg acgcgcaacc
     4201 ctcgggcgag atgaccgagt aggtcgcgta ctgcaacatg agggtcacgt cgcccaccgc
     4261 aatcgcgagc gccccgcccg aaccgccctc gccaatgatg gtggcgatga tcggcgtgcg
     4321 cagccccgcc atggcgtaga ggttgcgtcc gatggcctcg gactgcccgc gctcctcggc
     4381 gtcgatcccg ggataggcgc cgggcgtgtc gacgaaggta aacacgggca gctcgaactt
     4441 ctctccgagc ttcatcagcc gcagcgcctt gcggtagccc tcgggcttgg gcatgccgaa
     4501 attgcgcagg atcttctcct tggtgtcgcg ccccttctgg tggccgatga cgacgcaggg
     4561 caggccgtcg aagcgcgcaa ggccgcccac gatcgaagcg tcctcggcga agctgcggtc
     4621 cccgtgcagc tcttcgtagc cggtgaacag tgttcccacg taatcgagcg tgtaggggcg
     4681 ctgcgggtgg cgtgcgacct gcgccacctg ccagggcgtg agcttggcgt agatctcctt
     4741 ggtgagcgcc tggcttttct tcgccaggcg ctggatctcg tcggagatgt cgaccgccga
     4801 atcgtcctgc acgaagcgca gctcctcgat gcgctgctcg agctccgcga tcggctgctc
     4861 gaagtcgagg aaggtggtct tcatccgcgc gcgagattct agcgcaggac gacgacctgg
     4921 tttctgcccg catgcttgcc gcggtacagc gccccgtcgg cgcgcgccgt cagcgcctcg
     4981 ccgctttcgc ccttgaggtg ggcggcgatg ccgatcgtca cggtcacgcg gcgctgcgcg
     5041 ggcaagcccg ggaactcgcg cctctcgacg ctcgagcgca ggcgctccgc cgtcgccgcc
     5101 gcaccggacg cctcggtatc gggcaggatg agcatgaact cctcgccgcc gaagcggccg
     5161 aagacgtcca tcgcgcgcaa gccgctgctc gcgagcaccg cgaagtgcct gaggaccgtg
     5221 tcgccggctg cgtgcccgag cgtgtcattg atgctcttga aatgatctat gtcgatcatg
     5281 cagaccgaaa gcggcctgcc cagacgtgcc gcgcgcgcga tctcgcgctg cagcatgtcc
     5341 agcaggtggc gccgattgtg cacgccggtc agcacgtcgc gaatcgcgtg ctcctccgcg
     5401 cgatcggcgg cttccttcag ccgccggttg ctttccgaga gccgcaggcg cagcccgttg
     5461 acgtatccgc ccatcaccgc gaaccacggc agcacgacgg cgagcgctgc gagctgtagc
     5521 caattggcgc gtgcgccttg cccctgcagc gccccggttg cgaacagcac gcccgcatgc
     5581 gcggcgaggg cgatgagcgc cagcacgagc agccgcgcag tacccagccg cagcacaccg
     5641 aagagcagcg ccaccagata gaacaggctc agcgcatcgc gcgcggacgc cgcgtggtac
     5701 atgaggtagg cgagcgtcat cagcgcagcg acgatcatct cgctcgtcag gctcggatcg
     5761 cgaaagcgct gattgaggcc cgagcggaac atgccgtaga agagcacgat gaaaaaaccg
     5821 atcagcgcag cgccgccgat ggcgatcttg agcggcagca ggccgaacca gtgccagagc
     5881 aagagcgacg ccgccacgag aagcgacgtc cccgcgccca tgaggtagcg ccgcagacgc
     5941 agcgactggc gcgcctgctc gtctccgagc gcggccgacg acgccagcgt cccttcgctc
     6001 atctcaatat tctaccggca gcggatcgag cgaccgccac aggtaccagg tcgcgaccga
     6061 gcgccagggc gcccattgct cacccagccg ccgcatgcgc gcgggcgatg tccggcgccc
     6121 gccaaaatag tggcgccgca atgccgcctg cagccccagg tcgtcgagcg ggaacacgtc
     6181 ggggcgcaac aggttgaaga tgagaaacat ctccgcggtc cagcgcccga tgccgcgcac
     6241 ctgcaccaga tcggcgatga tctcctcgtc ggacaaggcc ggccagcggt gcgggtgcac
     6301 gaggccccgg tcgaagcgcc gcgccagatc gacgatgtac tcggtcttgc ggtccgacag
     6361 gccgcaggca cgcaagctgc gcggctcccg ccgcaacacg gcttgcgggg tgcagtcgcc
     6421 gacgcacgcg atcacgcgat cccagacgct ttgcgccgcc ttcaccgaga tctgctggcc
     6481 gacgatggcg cgcgccagcg tcatgaacgg ctcgccgcgg cgcaccaggt gcacgcgggg
     6541 gcacgcgcgc atgatcgccg ccaagactgg gtcgcgtcgc gccagcgtgc gcttggcccg
     6601 gtgccagtac tcaggcttca cgcagtggga ggttgagcag caggttctgc ggcttcatgc
     6661 gcaccacctg cgcctcgttc acgctcaagg cgagatagac ggtgcggcca gacacgcttt
     6721 cccgcatcag cgccggatcg gcgaactgca tcgagaacag cgcgacgatg ttgcgcatgc
     6781 gcccggcatc gacctggctg cccgaccata gctcgttgag aatgtccgtg gactctgcat
     6841 cgagcccgat gtgccaggcc cagcgcgcct cgtcgatctt gcgcagcggc ttcaccctca
     6901 cctcgacggc atggaaatgc gcgatccagc cctcgatcac ccggcacagc gcctcgagcg
     6961 caggccggcc gtaggtcagg ctgatgaccg tgtcgtggcg cgattcgcgc tcccagtaga
     7021 gcgccgcgtt ggcgcggtcc agcacgtcga ggtcgacctt gccgagggtc ccttgcgcct
     7081 cgacgatcag cttgccgatg ctgccgtagc ggttgcccgc ggcgtgcatt tcgacggtct
     7141 cgtggtcggc gagcagcaca tgcccgtcct gcagggtggc cttctggtcg cggaagaaga
     7201 gctccgcggc gcgcagccgc agcgggtcct cggcgccatc gaggatgttg cgcaggatga
     7261 cgtgcgccag ctgctcgatg aacagcggcg gcacgtcgat gtcgcccttg aacagggtca
     7321 tgtagcagcc ctcgagcgtg cccgcggcga gcaggcggtc gcggaacctg agcagcatgc
     7381 ggtagttgtg ccgggcgtcc tggtcggcaa gcgcgtcgat ctcggcggcg gccaccgcac
     7441 ggcgcggcgt ctccatcaac gacgcatgca gccgcagctc cgcgtcgcag gactcctcga
     7501 ccgggtggat ctccggccgc aggtagtagg cgcgcaggaa gtcgtcggtg acccgcagcc
     7561 ggccggccgg gtcgcgctct aggaggtgga aaccgctatt gcgccagaaa tcaggcaggc
     7621 gacgcgacca accgcgaatg cgccgcgacg tgctggcgca ggaactccat ctggtcggcg
     7681 aagatgcgac ggttcgtgag gatcaggtac tcggccttgt tcggcacgta cggcgcgtac
     7741 atcagctcca tgccgcattc ctgcggcatg cggttgcgct tgtggccgtt gcagtgccgg
     7801 cacgcggtca ccacgttcat ccaggtgtcg cgcccgccgc gcgacagcgg cgtgatgtgg
     7861 tcgcgcgtga ggcggaagta gccgtactcg tggccgcagt aggcgcaggt atggcggtcg
     7921 cggcggaaca gctcccggtt gttgagcggc ggcacctgat ggaagccgcg tgcggcgagc
     7981 gcgcgaccgc ggatggcgat gatcgagtgc gcggtgatgc tggagcgctc tcccgtcagg
     8041 cgcgacatgc cgccgcgaaa cgtgaaggtg cgctcgccca gcgtccaggc aacgaggttc
     8101 ttggcgtagt agaagcaggc ctgctgccag ctgatccagc gatggggcac gccgtggcta
     8161 tcgagcgaca ggatcagcag gttgtccatc accgccgaga ttctgccaag ggttcagcaa
     8221 cttggcaatg atcccgccag cgagacgcgg gcggcctcag cggctggcga cgcggtcctt
     8281 gaagccttgc ccggcggcga aggccggcac ggtcttcgac gaaatgcgga tctccttgcc
     8341 gttcaatggg ctgcgcccgg cacgggcctt acgcttgcgc ggcaggaagg tgccgaagcc
     8401 caccaccgtc acccgattgc ccttggccac gcttgccacg atcgcatcga acaccgcgtt
     8461 caccgcttct cccgcgctcg actttgtcag gttgccggcc cgtgcggccg cttcgacgag
     8521 atcagccttg gtgacggatc gcatggcgtt gttgtctcca ctaacttcaa gcgagccgaa
     8581 atcataatca gatcgccgcg cgaatacaac gccaaaagat cgcgtgcgcg caaagtcgtg
     8641 cgcggacgtt gaaggaattt gtgctagcgc ttacccgcga gcagatcgac gagcgcgctc
     8701 ggttcatgct cgccgcgtcc ctgcgcgacg agcaggcggt agagctccgc cgcagtgcgc
     8761 gccatcggca gcggcacgcg cgcgcccttg gccacctcgg cggcgttctc cagatccttc
     8821 agcatggtcg ccacggcgcc gagcggcggg tcgaattgcc gcgccgccat gcgcgcgcca
     8881 aatacctgaa gcggcagcga atcggcgaag ccacccttca gcgcctgcgg cagcgcgtgg
     8941 gcgtcgacgc cggcggcttc cgcgagccgc accgcctcgg cgatcaccgg gaacaggcac
     9001 ccgacgatga cctggttgca gagtttcgtc acctggcccg cgccggcgtc ccccatgcgc
     9061 gtgaagcgct gcgcgaggtg cgcgagcacc gggcgcgcgc gctcgatgtg ctccggcttg
     9121 cctcccgcca tgatggcgag cgtgccctgc tcggcgccca ccgtgccgcc cgacaccggg
     9181 gcatccacca gccccatgcc gcattcgcgc tcgagacgcg tggcgaactc gcgcgccgag
     9241 gacggcgcga ggctcgaaaa atccaccaac agcttgccgg gagcgccgcc cgcagctatg
     9301 ccgccgggcc cgaacaacac ctgcccggcg gcgtgctggt cggtcacgca catcatcacg
     9361 atgtcggccg cacgggctac ggcagcgggc gaatccacgg cctgcgcgcc tttcgccgtc
     9421 agcggcacga gcttgtcccg gctgcggttc cacaccgtga gcgcgtagcc cgcggcgagc
     9481 agccgcaggc tcatcgggcg acccatcagg ccgatgccga tgtaacccag gcgcggctgt
     9541 tcgctcattt cctccctccg ctatgggtaa ccgcgccgcg atgcgccgac ccgaccaccg
     9601 cggcatactt ctcaagcacg cccgagagcg gcgcgcgccg cggcgccttc atcttgcgcc
     9661 tgcgcagctc gacgcgcggc acgagcagcg tcaactcgcc tgccgcggca tcgatgcgga
     9721 tgcggtcgcc gttcctgacc agggcaagtg cgccgcctgc cgccgcctcg ggcgacacat
     9781 agccgaccat catgccccgt gtcgcgcccg agaatcgccc gtcggtgagc aatgccacct
     9841 tctcgcccgc cccctggccg tagatgagcg ccgtgacgcc gagcatctcg cgcatgccgg
     9901 ggccgccttt cggtccttca taacggatca ccagcacgtc gcccggccgg tacctgcctc
     9961 ggcgcacgac atcggcgcag gcctcctcgg agtcgaatac gcgcgccggg ccctcgaaca
    10021 cgagcgtctt cagacccgcc accttgatga gcgccccttc cggcgcgagg ttgcccttga
    10081 gcaccaccag ccccccggtc ttcaggatcg gggcctttcg caccacgtcg ccgtcggccc
    10141 ccgcatgatc cgccagggcg gcttcgagcg tgctgccgga gagcgtgagg cagccgccgt
    10201 gcagcgcacc gcgctcgagc agcgccttta gcacggcata cacgccgcct gcggcgtgca
    10261 ggtccttcgc caaatagcgc ccgccgggct tcatgtcggc gatgagcggc gtgcgcttcg
    10321 ccacgcgcgc gaaatcgtcc atgtcgaagc ggataccggc ctcgtgcgca atcgccggga
    10381 tgtgcagcgc ggcgttggtc gagccgccgg tggcggccac tgcggcgcag gcgttctcca
    10441 ggctcttgcg cgtcaccagc tcgcgcggcc gcggaccgcc atcgcgcagc agccgcatca
    10501 ccgccgcgcc ggcggcacgc gcggccgcgc cgcgcgcggc gtccaccgcc ggcaaggtcg
    10561 ccgtgccggg cggcgccagc cccagcgtct cggacaccat cgccatggta ttggcggtga
    10621 actggccggg gcaggaaccg agggtcggca ggcaggcgcg ctccagctcg tccaggtcgg
    10681 aagccggcat gtctccggcg aggaccgcgc cgacgccttc ataggtgtcc agcacggtga
    10741 cgtcgcgccc gtgccagcgg cccggcagcg ctgccccacc atatacaaat acgcccggga
    10801 gatcgagccg caccagtgcc atcatcatcc cgggcagcgt cttgtcgcag ccggcaaagg
    10861 cgacgaggcc gtcgtaggcg tggccgcgcg ccaccgcctc gatgccgtcg gcgatcagct
    10921 cgcgtgacac cagcgagtag cgcatgccgc catggttcat cgacatcgag tcggcgaccg
    10981 acacggaagc gaaatcgaac ggcacaccgc ccgcctcgcg cacgccgtcg caggccgtgc
    11041 gcgcgaactc accgagcagc gcgttgcacg gggtcacgcg cccgtcggtc gaaacgacac
    11101 cgacgaaggg cttgccgagg tcggcgtcgc cgacgcccag gcttcgcagg aatgcgcggt
    11161 gcggcgcgcg gtccagcccc tcgaccgtga cccgcgaggg caggatcgga atgctccgcc
    11221 gcgccacgct cagtcgccgc gcgcgcccga gagctgcacc acctccgacc atttcacgac
    11281 ttctgccgcg atcaccgcgc cgaacccgac cggcgtgctc ggcgcgggtt cggcacccgc
    11341 ggcgatgatg cgatcgcgca cctcggcgcg cttcagcgcc gcgacgaaat cgccgttgag
    11401 ccgcgcgacg atcgcgcccg gcgtggtcac cggcaccacg atgccgagcc aatcaccggc
    11461 ctcgtatccg ggcagacccg cctcggcgaa ggtcggcgcg tcgggcgcgg cgaacacgcg
    11521 cttcgcgctg ctcaccgcga gcgccttgag cttgcccgac ttgacgtgcg gcagggccga
    11581 ctgcacgtcg accaaggcga gcgagagctt gccgttggcc gcgtccgccg ccgcggcgcc
    11641 atcgcctttg tacggcacgt tcaccatctg cactttcgcc atcagcttga acagctcggc
    11701 cgccaggtgc gtcgcggtgc cgttgccgcc gaagccgatg agcatctcgc ctggcttggc
    11761 gcgcgcgagc gccaacagct cctcgagcgt cgctgccggc agcgtgggcg gcgcgaccag
    11821 cagaaaagga aactgcgcga tgctcgtcac cggcgccagg tccgtcaccg ggttgtacgg
    11881 catcttcgcg tacaggctcg agttggtggc aagcgcgccc gagccgccga ggccgatggt
    11941 gtagccgtcg ggcgcggctt tggcgaccag gtcgatgccc tcgctgccat ttccgccggg
    12001 cttgttctcg atcaccaccg gctggcgcag ccgctccgcg agcagcggca ccatggcgcg
    12061 cgccagcacg tcgccgggct cgccgggcgc aaacgcgacg acgaagcgga tcgacttcgt
    12121 cgggtaggtg ggctgctgcg ccgccgccgg cgcgatgctg agcgcggcgc atgcggcgag
    12181 cagcccgcgg gtcacgagtc tcatgcagcc tccttgaagc gtgaagggta gcctgcgcag
    12241 gcccggtgcg ccagtgtcgt gcgccggcgc cgctcagcgc ccgagcgtgg cgaaggtgcg
    12301 ttgcagggcg tgcacctgcc ccagatagcc gcccacgttc tgcccgccgc agcgctggtt
    12361 cgagcccagc tggaaccccc gcttcgcgaa tgcctcgcct gccgccaccc cgcacaaatg
    12421 catgatcgcc gccaggtcct gcttctggcg cagcgtggcg tcgatcttgc gagcctcgag
    12481 cgtgcgggac acgctcttgt cgagaaaggc ggccgtcatc tccgccgcgt ggctgggcac
    12541 cacgcggaag tacagcccgt tgaaccagca cgagcgccag tcgtgccacg gaccgtcctc
    12601 ggcgacttgg ttgtcgtgga tgcagtagcg cttcgcctgc tgcaaggtgc cgtcggtgat
    12661 ctggtacatg ccgaccgcgc tcgaggccgg gcggtagacg tcgaacggat cctcggtgag
    12721 ccgccagcgc cagggcgtgc gcgcgagcgg gttgcccgcg ccctcgacct gcgccagcgc
    12781 ggcgaggaag tcgggggtga tgacggcggt ggcgtgcgcg cggaagatcg gctcgtagct
    12841 gcgccaggtt tccgccggcg ccttgtcgag cgcgccgctc gccggcgaga agatctccgt
    12901 gggcttgcgc gcgagttgca ccagcgcgtt ggcggcgagc cacagcgcga cggcgagcgc
    12961 aagcgcgatg ccgatctgcg accagagcgg cgcgtggcgc agcctgtgcc acgccgtgcg
    13021 cgcgattccg ggacgtttgc tgcctttcag ttgaggaccg agtagccccg gttgcgcagg
    13081 cagttcttca ctacccgctg gcgctcctgc cgggttgcgg cggcgcaggc gctggcgccg
    13141 atcagggtca cgagcagcag agcgggaagg atcttcatcg ccgctagtgt agccgcgctc
    13201 agccgatctt gatcgcgttg ccgctacgcg ccgacgccac caccgcctcg gtccccgcca
    13261 ccacgttcac gatctcctcg ggcgcgatca cgaatttcaa cccgcctgcc gccgcgtcgg
    13321 cgaacgcctc cagctccgcg cgctccttgt cggtcgccgc gaaggcgaag cgccggacgt
    13381 tgccttcgag atcggagagc tcgagcgcgg tatcgccgcg caattccagg gctcccttcg
    13441 cgccgaacgc gtgcaggcgg tagaaccggg tcgccgcgtg cagcgtcgcc agggtgccgg
    13501 tcgcgccgcc cgcgaagcgc aacaggcagg aggtcgtgtc gtcgacatcg atgtcgagct
    13561 gctggcgctc gctgatggcg agaacttcgg tcgcgagccc ggcgagatgc accatggcgt
    13621 cgagcacgtg gcagccgcgt gcggtcatcg agccgcccgg gctctcgatg cggttggagc
    13681 gccagttgcc cggcgcgatc tgatagctcg gcgggccgga gaagttgccc tcgatatggc
    13741 gcagcgcacc gatctcgccg gccgcgatgc gccgcttcat ctcggcatag gccggcgcgt
    13801 agcgccgatt gaaaccgacg tgcagcgtga tgccggccgc gcggcaagcc tcgatcgcgc
    13861 gctccgcgct ctcgcgcgtc agggtgaagg gcttttccac gaacacgtgc ttgcgggctt
    13921 gcgccgccgc cgcgatctcg gcggcgtgct gcgaatgcgg cgtcgccagc accaccgcgt
    13981 cgatggcgga atcggccagc acatccgcgt aacgcgatgt cagcgccaga ccgaagcgct
    14041 cgccgagcgg tttgagtgcg gccggatcgc gggttaccgc cgcgacgaag cggatcttcg
    14101 ggctcacgcc ttgaaccgac tcgatcagcc gcgtgcccca gttgccagcc ccgcagatgg
    14161 cgacccgcag caagcggtca gcctttcgcg aaagacacca ggatcttgcc ggagcggccg
    14221 ccgcgcgcgg cgtgcgccac cgcttccttg atcttaccga tcgggtacac ggcctcgact
    14281 tccacggcga gcgtgccgtc ggccacccat gacgtgagct cgcgatacag ggccgcgacc
    14341 tgcgcgggcg gggtgtccac ataccacttg cgcagccaga agccccgcag atgcacgtcg
    14401 cgaaacactg tatcccgccc gtccaccatg cagggctcgc cgctcagcac gccgtagttg
    14461 accaccgtcc cgccgtcgct cacgcaccgg gcgagccgct gcgtggccga gccgcccacc
    14521 gcgtcgatgg cgagcggcag cggcgcctcg ccgattgccg cccgcacgcg cttgtccaga
    14581 tccggtccgt cggtgagcac gacgttgccg ccgaacgcct gcaacggctt tatcagctcc
    14641 tcgcgacgca cgacgttgac cgtcttcacg cctgcctgac gcgcgagccg gatcaggcag
    14701 tgaccgacgc cggagttggc cgcgttctgg atcacccact gcccgggctt gggcgcgacg
    14761 aactgatgca gcatcaggta ggcggtcggc ggattcacgc gcagcatcgc gagctgcttc
    14821 gggtccacac cttccggcag cgcgaagacc gccttcgcgg gcgccttgat gcgctcgcgc
    14881 cagcacccgg ggccgggcag caacacccgg tcgccgggct ttacgtggcg gaccgccgcg
    14941 cccgccgcgg cgatgcgccc gacgccttcc gcgcccggcg tcatgggcag cgcgggcggt
    15001 accgcgccgt acttgccttc gatgttgagg acatcagcgg gattgatcgg gaagaactcg
    15061 gtctccacga ccacttcttc ggggccgggc gcgccgggat cgggctgctc gagcacctcg
    15121 atcgcggctt cgggtcggcc gaacgcgcct acgtgagcgg cgagcatgga gtcatcgtat
    15181 tgcatttctc acgcgccggc caagacatgc cggatgccct gggcgcaagc gtgcaatctc
    15241 tgttcggcca tgctgaccgt cgacgaggtc cgcgccgtac cgctgttttc gaagctcgcg
    15301 acgctccagc tctcccgccg cctggctggc accatgaact aggcgcggct gggcgtgtac
    15361 agctcgatgc cgttgccttc cggatccgtg atcctgaagg acgtgaattc cggtcccgcc
    15421 ttcggcgtgg catcgaactt cttcgcccat tcactgagcg cctcgagcga tggcacgcga
    15481 aagcccatgt gcagctcgtc ggggctgcga ttgaccggcg cgcccgaatg gaacgcgatg
    15541 agcaggccgg gcccgcgcac caggtgctct tccgccttta gggaaaacgt gcgcgcgtac
    15601 cactgggcca gaccgagcgg atcccgggcc gggatgttca tgtgatcaag acggatgtcc
    15661 gacacggtgg attgcccttc ggtcaaggat tgcagagcgg catcttaatc cacgcacggc
    15721 cccttcgttc gcaaacgaat ccggccaagt cgtcggcgcc atcaatcggc tgccgccgaa
    15781 accccggatc cccgttcgtc tatccgccac ctggcttgca gcgtgcgcag cagcgcgaca
    15841 caagcaacaa cgctcgccat gagcagtgcc gcgagcagca ccggggcctt cgcgctcccg
    15901 gtccagtcgc gcagccagcc cgcgagcacc ggcatcagcg tcatgccgag gtagtaccag
    15961 gtgtagaaga tgccgagtcc ggggcccctg ttctcgcggc ttacggcctc ggccgacagc
    16021 gacatgagcg cgccagccgg tattccggaa atgattccca ccaggacaag acctgcctca
    16081 ggcaatgttc cggtggtgat gacgacgagc atggccgacg aggccagcag cagcgccgcg
    16141 agcgccagcg tgacatgcct ggtggactgc agcagtcgcc cgccgatcgg taccgagacc
    16201 atcatcgtcc acatgaacag gctggtgacc gaactggcgg cgccgcgctc gtagcccagc
    16261 gatatgagcg cgcccggcat gaagctgacc acaagaatga acgcagcgtt gtagagcgtc
    16321 caggccacgc cgaccacgct ggtgtggaca agctgccgca ttggcagccc gtatctcagc
    16381 ggctcgccgt gcagatcaag aggtgcgggc ggtgcgcggt agaacagcgc ggtcagcagc
    16441 aaggccgcca gcgccgcgag cgccgtgacg tacatcaccg agttccagcc gtaggcggcc
    16501 gcgaccggat tgtgaatcag cagtccgagc gcgatgccga tcggccaggc cgtcagcatg
    16561 atggccagcg ccggcacgat ctctttctcg aagaaccact cggtgaccat cttcgtgaga
    16621 atcacgttga agatcgtgcc gcccacgccg gtgatgagcc ggccggcata cagctcggtc
    16681 gcgtccgcgg cagatcccat cacgtaggcg ccgaccgtca tcgtcactgc gccgagcatc
    16741 agcaggttct tgtcggagat cgcgcgcgtc agggcgccgc tgggaatcgc gatgaacacc
    16801 cccggcagca ggaacaggcc gatcagcgtg ccgacttgcg cgtaactgag gccgaaggcg
    16861 tcaatgagct gcgcggaggt cgaggcgacc gactggaact ggaagcccat cgccagccgg
    16921 acgagaaaca ggatgagcag catcagccag cgcacgggca cctccctgga tcggctcgca
    16981 cccgggcccc acaggttcga gatactgccg actcgtgatt ccacggacgg cgtcgaagga
    17041 tatacttcac cacgcaggat caaaacaaag aatcggccgt agcagaccga ttcgttgcct
    17101 cacccttcgg gtccgcaggt cccgctttta gggtacagcc tgtaccaccc agcgagctcg
    17161 ccggcgcgtt gctttgcgca gccgcgcatg gtacttcttg aagcccagga gatcacttta
    17221 gaggaggagg acgtcatggc actgttcatg ttgatgggaa agtactcacc ggaggcgatc
    17281 aaaaccatta tggaaaccgg ctcggatcgg gaggcggccg ccaggacggc gcttgaagcg
    17341 gcgggcggca agctgctcgg cttctacggg atgttcggcc aggactacaa cgtcgccatc
    17401 gtcgcagagg tgccgggaaa cgccgaatac atcggggcga tcgctcctgc gctgatggct
    17461 ggaaccttca cggcgttcaa atcgatcccg ctttacaagg cgagcgacgt ggtgaaggcc
    17521 tcgcaaatcg ccaagaaagt aagcgcagcc tacacgccgc cgacgaaagc aaagtagaag
    17581 acggacgccg cggcgtcgaa acgaaaaggg ccagcgcttg ctggcccttt gtatttggcg
    17641 cgcccggcag gatttgaacc cactgagctg accggctttt tctgtaccag gttgagtgta
    17701 gtagggttca acttggaagg agaaggcaga tggcgaagaa gaagtacagc cccgagcagg
    17761 tggtggcgaa gcggcgccag atcgaggtgc tcaccggcgg cgggaagtcg atcccgcagg
    17821 cctgcaagga ggccgggatc accgatgtca cgtactaccg ctggcgccga gagtacaacg
    17881 aggtgaggcc gcacagttcg cgggctaccg gccgccggcg ccggcggccg gtagcccgcg
    17941 agccctcatg ctcgcgcagc cggagatggt gcaataaggt aacaacggtc ctgttacaaa
    18001 gaatcggtca ggtcaacgag ctggtcgtac gcgcgacttc acgtgcccta cccaacgcaa
    18061 gaacggccga gcgggcgaat tgcgggaatc cttcagcgca tacacgtaat agaagttcgt
    18121 ggccacacaa ccccttgact atcgtcccga tgcccgccac tctcaaggcg caactggatt
    18181 ctgatcgtgc cgcagactgc gcatcgctgc ccacgcttcg gcgcgaatcg cttacagaag
    18241 cgaatccctc ggcgcatacc gctcgaacgc ctacgccaaa ccacgccgga cctgctcggc
    18301 gttcgtgtcc acgccagcgc agtacacgcg ccctatagcc tgtccgtagc ggtcgcgccc
    18361 gtgattgtcg aaccgtcctt gaccgcgcgt gttgcacggc acaaggccga aggaacttcc
    18421 tatgctcttg cgttgaatca agccccgcga tcccgctcgt ggtaggagtg ttcatccgcg
    18481 catgttcaac cgggatcgac cgaaggtgat ggccgcgcta cccgagctga tcaggccgct
    18541 cgccgacctc gtcgacgaga acgggatgct gtcgggagac gatctcgcga gccgcaagcc
    18601 cggctggttc atcgacaagc tcagggccga cgcggtgttg cggccgaccg cgaccgaaca
    18661 ggttgccgcg attctcactt attgcaatga acacgcgatc ggcgtcgtgc cccagggcgg
    18721 catgacgggg ctcgtcagag ccacggagac cgaagcgggc aatatcgtgc tgtcgctgga
    18781 gcgcatgaat cgtatcgagc acatcgatat cgtcggccgc acggcgcggg tcgaggcggg
    18841 cgtgatactg caggcattac aggaaaagct ggccggctgc gaccttgctt tcccgctgga
    18901 tctcggtgcg cggggcagct gccagctcgg cggcaacgcc tcgaccaacg ccggcggttt
    18961 gcgtgtcgtg cgttacggca tgatgcggga tctcgtgctc ggcatcgaag ccgtgctggc
    19021 cgatggtacc gtcgtgagct cgatgaacga aatgctcaag aacaacaccg gctacgacct
    19081 caaacagttg ttcatcggtt cggaagggac gctcggcatc atcacgaggc tcgtgctgcg
    19141 gctctacgaa gcgccgaaaa gcaggagtac ggcgctcgtc ggcgtctcgc gcttcgagca
    19201 ggtgacggga ctcctgaaac acatggacag cgcgctggcc gggcaactga gcgcgttcga
    19261 ggtgatgtgg aacagcttct atgcgttcaa cacgcgattt ctcggcgacg cacccctcgg
    19321 caccaactat ccctggtacg ccgtcgtcga atcgctgggc ggcagcccgg tacgcgacca
    19381 ggcccagttc gaggccgcgc tcgagtcagc gctcgaggcc ggtctggtcg acgatgccat
    19441 cgtcgcgaaa tccgatgcgg atgcgaaacg cttgtggcgc gttcgcgaaa gcatcgagtc
    19501 gatgatgggc cgaccggtct tcgtctacga tgtcagcctg ccgatcgtca ggatggaggc
    19561 ctacattcgc gaactcgaga agcggctgcc acgcgagctg cctggctgcg aatacgggac
    19621 cttcggtcac ctggccgatg gcaacctcca cgtcgtcgtg ctgctcgacc ggaaccagca
    19681 gggcggcggc ctcgccgagc tgcacgtcgt cagtgatcgc gcggtgtacg agcctctcgc
    19741 ggcgatcggc ggatccgtct cggcggaaca tggcgtcggt ctcgagaaaa agccttatct
    19801 cccgctctcg cgaagcccgg cggaactggc gttgatgaag cggctgaaac agctgctcga
    19861 tccgaacgga atcctgaacc cggggaagat cttctaggaa aaggggtcgc gcatgaccca
    19921 gtcaccaacg cgtggcgtcc cgtgtccggc tgtggctcgg cgccgcgatc acaccacagc
    19981 gtcgtctgga cgggcggcga aatgatcgtc tggggcggcg gcaatttcgc gtccgtccgg
    20041 tagggcgccg gggcaaggta cacgccatag tcacccgtag cgcgggtcat cagtctggct
    20101 ccgctcagcc ggcgttgcgc tggcggcccg tagcccgcga gccctcgtgc tcgcatagct
    20161 caaaatggtg cagaactgta ccaacgaccc cggcaaaagt gatcggtcag ctcaacgtac
    20221 tagggcatca cggcggaccg gactcgcgga tcggccaata cgtctcggac acgcccctgc
    20281 gcatcccata ccacctgtaa cgtgtcgaac ttcccatacc agtacttcca gcgatcggcg
    20341 tccaaagacg gcgtctcatg cgccggcgga tatcccaggg aaacgatgac ttgctcccgg
    20401 gtcatgccaa tcgcgacctt gccggcgctg acggcgtgtc gcgccgattc gggccagccc
    20461 gcgatcctgc tcctgggatc gtcctgcacg atcatctttc gggcccagtc ggtgatgctc
    20521 tgactccgcc cgtaatccaa gcctagatgc atctcctgcg tgtacatcac gtcgacgtcg
    20581 accgtgatgt gatgctctga gtactccttc gtgcgaatcc gggtgccggc cgggatcatg
    20641 ggcagcgagg accaattggc gtcgctgatc caatacctgt ccgcaagtcg gcgggtggcg
    20701 cccacgttcc ttctggccct ttcttagtgg aagttgcagc aggtatatcc gctcagtgcg
    20761 ggcccgtgcg tgacgcaacc ggaattggta cctgccagga ttggcgccag catggcagat
    20821 gaagccagcc caacgcgcca ccctctcatc gactttaggt tcatgggcac gaccctccgt
    20881 ccgcacgcct aggcgaggat ccctccaaag gcccgttcgg cgcaagcggt gcaggtggcg
    20941 tccccacggg gattcgaacc ccggttaccg ccgtgaaagg gcgatgtcct aggcctctag
    21001 acgatgggga cttgtgcgga caacaagtct ggtctggtgg aggtaaccgg gatcgaaccg
    21061 gtgacctctt gcatgccatg caagcgctct cccagctgag ctataccccc agcacagggc
    21121 gcgaattata gcccacgaac gctagggctt acagggcccg agccgacgcc cgcgcgtctt
    21181 cgtcaccacg ccgcccgtga gcttcagctc gccagacatg ctgttgcgcg agatctgcgc
    21241 ccgccccgcc ccgcgcatgt tcgacgccgg gcactccagc gcccacgagg tgtaatcagg
    21301 cccgagcttg agcgtcgcga cccggcaatc cgagggcacc tgcgcgccgc ggccccagta
    21361 catggggtcc ctcgcctgct cgggcgtgag gcacgcgcgg tagcctgact gctgcggccg
    21421 cggcagcccg ggcatggcga tctccgacac gaactcccac tcgcccggct ccatgcgctg
    21481 cgcgtgcgcg cccgccgaaa cgagcgcgag cagcaggacg atccccctca cgcgagcgcg
    21541 cgtccgatgc gcgcgagcac ggcctcgcgc ccgaggagct cgagcactgc gtcgatcgac
    21601 ggcgtctgct cgcgcccggt gagcacacgc cgcaggggca tcgccagctg cggcatcttg
    21661 aggttgtgcg ccttggcggc ggaagacacc gcggcgccga gcgccttgcg ctcccaggcg
    21721 agcgactcga gttccttctg cagcgccgcg agcgccggct tcgcctccgc aggcacgctc
    21781 tgcgcgcccg gctcgcggta gaacatctcc gcctgcgccg cgagctcctt gaccgtgccc
    21841 gcgcgctcct tcagaagcgc gaccaccgcc ggtcccggct cgcggccgag aaacggcgcg
    21901 gcgagcgcgg caagccgcgc ctcatccgcc tgcttcatgt actgctgatt cagccaggcg
    21961 agcttttccg ggtcgaactt cgccggcgag tggctgacgt gcccgaggtc gaaccactgc
    22021 tcgagctcgg ccatcgagaa cagctcgtcg tcgccgtgcg accagcccag gcgcgcgagg
    22081 tagttggcga gcgcctccgg caggaacccg tcgtcgcggt actgcgtgac gctcaccgcg
    22141 ccgtggcgct tcgagaggcg ctccccgtcg gcgccgagga tcatcggcac gtgcgcgaac
    22201 tcggggagcg tcgaccccag ggcgcggaag atgtggatct ggcgcggcgt gttgttgacg
    22261 tggtcgtcgc cgcggatgac gtgcgtgatc cgcatgtcga ggtcgtccac caccacgccg
    22321 aagttgtagg tcggcacgcc gtccgcgcgc agcagcacca ggtcgtcgag ctcggcattc
    22381 gggaactcga tcgggccctt gaccaggtcc ctccaggcga ccgtaccctc ctgcggcgtg
    22441 cgaaagcgta cgggcgccgg gccttcgccc ttgtacttct gcccgatcgc gcggtcgtag
    22501 cgcggcttct cgccgcgtgc gctctgctcg gcgcgcagcg cgtcgagcgc ctccctgggc
    22561 atgtagtcgc gatacgcgtg tcccgccgcg atcagctgct cggcgacttc ccggtagcgc
    22621 ggcaagcgct gcatctggaa gaacggcccc tcgtcccagt ccaggccgag ccactgcagg
    22681 ccctccagga tcgcggcctc ggattcgcgc gtcgagcgct cggtgtcggt gtcctcgatc
    22741 ctcagcacga acgcgccgcc gtggcgccgc gcgtaggcgc gcgaaaacag cgcggtgcgc
    22801 gcgccaccga tgtgcaggaa ccccgtcggg ctgggggcga agcgcgtgcg cacggtcatg
    22861 ggtgctgcga ttttcccatg tccgcgacgg tccaacccaa tcgccgggga caagtcccct
    22921 atacctatgg cccgaggccc cgcagaacag gtaaaatcgc gccctctccg gcagggtcgt
    22981 tagctcagcg gtagagcact gctctcacac agcaggggcc actggttcga tcccagtacg
    23041 acccaccatt cgacgccgag gcttccttgc ccgaatccgg cgccacgcgc attcgtcttc
    23101 ccgggaaaca cgaggccgac ccctcgctcg cgcacggcgc gggcctgacg gcgaagcagt
    23161 tgcaggcagt ccaggacgac gcctggtccg accgcacgct gtggaccgag gaggagtggc
    23221 tcgtggtgcg ctatgccgag gcgctcgccg cggtgccgca ggaggtggac gacaagctgt
    23281 tcgcggcgct gaagtcgcgc ttctccgagc gccagatcgt cgacctgacg atgcgcctcg
    23341 cgctgtgcgc ggcgtggaac aagt
//
LOCUS       DBNHKE010000241         5806 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_787950, whole genome shotgun sequence.
ACCESSION   DBNHKE010000241 DBNHKE010000000
VERSION     DBNHKE010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_787950"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC16O9_16480"
     CDS             complement(<1..770)
                     /locus_tag="AC16O9_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6518251.1"
                     /translation="MNKAREFDQSVLTELKALLGERVTTSSGIREHHGKDESYFPHAA
                     PDAVAFPLSTEEVRDIVNICRRHRVPMIPYGVGTSLEGHILAIRGGVCIDMGQMNKVL
                     AVHEEDMDAVVQAGVTRKQLNEFIKHTGLFFPIDPGADATLGGMSATRASGTNAVRYG
                     TMRDNVLSLKVVLADGRIIQTSRRAKKSAAGYDLTRLFVGSEGTLGIITEITVKLHPV
                     QAAMSAAVCAFETVKGAADTVIQTLQSGVPIARSELLCA"
     gene            complement(775..981)
                     /locus_tag="AC16O9_16485"
     CDS             complement(775..981)
                     /locus_tag="AC16O9_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HIR6518252.1"
                     /translation="MDQETFNLSIRKFLKMVGVNSQREIEQAVQQALADGKLKAEGPL
                     AARVTLEIPALGVRVPFEGEIRLK"
     gene            complement(981..2399)
                     /gene="pgi"
                     /locus_tag="AC16O9_16490"
     CDS             complement(981..2399)
                     /gene="pgi"
                     /locus_tag="AC16O9_16490"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR6518253.1"
                     /translation="MTTTPPTDCPAWAKLAAHAEGWRDTHLAELFRHDAERSARFGAH
                     APALLLDYSRQRVGALTLRLLAQLAAERGIEGWRAALFAGAPINVTENRAVRHAALRA
                     GDAAPSEVKAALLRMRALAAGLGGVKRIVHLGTGGSDLGARLLMDALREHARIDVRFA
                     ANIDPLDLSRALEGAEPATTLVVAVSKTFTTAETMANARAARDWLGGRGKLFAVSANE
                     AAARAFGAAEVLPMWDWVGGRFSIWSAASFSALAALGPDTFDEFLAGGRDMDRHFLEA
                     PLEKNVPVLLALLGAWNVNFLGAATHCVLPYAHALKLLPAYLQQLEMESNGKRVDREG
                     RALGYATAPVVWGAEGTVSQHSFHQLLHQGTQVVPADFIVAGVSAELDANAEAQARAL
                     AFGTTDPGLPPHRRYPGNRPSSTLRLARIDARALGALVAAYEHKVFVQGVLWNVNSFD
                     QWGVELGKQLAGSILKDQMGRT"
     gene            complement(2396..3985)
                     /locus_tag="AC16O9_16495"
     CDS             complement(2396..3985)
                     /locus_tag="AC16O9_16495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6518254.1"
                     /translation="MIPRLALPPAPAVLALIALAYVLPGLTGHDPWKAYDAIAIEIAY
                     QMHVSGDWVVPRLAGEPWLEDPPLYHWIALAFGKLAGWAVPFHSAARLASGACVLVAL
                     WLLYRTAGAAAPLLVLGSIGFMVHAHEALPELAALAAACAAYALLPQAHARPLLHGAA
                     FGAALGLAFLALGPLVPAALGLAVAAAHAACPAWRSWRALPFLAAALLVAAALAASWP
                     WALGARAPALSADWWSAATQRQGTPLDNLRYYLVTGSWFTWPAWPLAAWALWARRREW
                     REPRLFAPAAACLLTLAALAATGPPQDASVIALLAPLALLGAEGVARLRRGAAAALDW
                     FAVMCFTFFAALVWLGYVAMMLGVPPKIAHNFAKSAPGFEAQFAAAPFLLALALLVGW
                     LYVAFFTAPSPMRGVTRWAAGVTLLWGSFAMLWMPWADYQKSYRPVALQIRSRVPADA
                     GCIAGQNLGVSQRAALSYHGALRTEPASRAGADGCRLLLVQGSPRDERDGPDARWSKL
                     ADVGRPGDKNERYRLYRLDVP"
     gene            complement(4056..4319)
                     /locus_tag="AC16O9_16500"
     CDS             complement(4056..4319)
                     /locus_tag="AC16O9_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIR6518255.1"
                     /translation="MKTTGHPDYQPVIFLDTSSNHSFLSRSAIDTKGRETMKWSDGND
                     YPVVKVEVSSESHSFYTGKQKIVDTAGRVEKFKRRYTKAKKSA"
     gene            complement(4393..5727)
                     /locus_tag="AC16O9_16505"
     CDS             complement(4393..5727)
                     /locus_tag="AC16O9_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6518256.1"
                     /translation="MSAARPFPLVPAPRLLWAAALLFALGVLAALAPALGPAWTLVAG
                     AAALAAALDAAAGLRLPAPGVARRVPHSLALGVPFEVALRAANASARALRLEVNDLHP
                     ASFECEGLPQTVALAPGEWREVRYRARPLARGAVAFGETEMRLASPLGLWQVARRAGE
                     AAQARVFPNFRALARYTLLATDNRLSQIGVLQVRRRGEGMEFHQLREYRQGDSQRAID
                     WKATARTARLIAREYEDERDQRVLLVIDCGRRMAAKDDALSHFDHALNAALLLAHVAL
                     RQGDAVGVLTLGASGAPRYVAPRKSAATVNTILERLYDLEPSLAMPDYLRAAREVMAR
                     MRRRALVVLLTNLRDEDDDTLTPALGLLRKRHLVVLASLREAILGRALAARVDSFDRA
                     VTHAAAADYLAQRERAFGRVAAAGALCLDVEPERLAISLVNRYLELKRQGRL"
BASE COUNT          849 a         2049 c         2143 g          765 t
ORIGIN      
        1 gcgcacagca attcgctcct cgcgatcggt acgcccgact gcagcgtctg gatcacggtg
       61 tcggccgcgc ccttcactgt ctcgaacgcg cacaccgccg ccgacatcgc cgcctgcacc
      121 gggtgcagct tgacggtgat ctcggtgatg atgccgagcg tgccctccga ccccacgaac
      181 agacgcgtca ggtcgtaacc cgcggccgac ttcttcgcgc ggcgcgaggt ctggatgatg
      241 cggccgtcgg ccagcacgac tttcagcgac agcacgttgt cgcgcatcgt gccatagcga
      301 acggcgttgg tgccggaggc gcgcgtcgcg ctcatgccgc cgagggtcgc gtccgcgccc
      361 ggatcgatgg ggaagaacag gcccgtgtgc ttgatgaact cgttcagctg cttgcgcgtc
      421 acgccggcct gcaccacggc gtccatgtct tcctcgtgca ccgccagcac cttgttcatc
      481 tggcccatgt cgatgcacac gccgccgcgg atggcgagga tgtggccctc cagcgaggtg
      541 ccgacgccgt agggaatcat cggcacgcgg tgccggcggc agatgttgac gatgtcgcgc
      601 acttcctcgg tcgagagcgg gaacgccacc gcgtcggggg cggcatgcgg gaagtagctt
      661 tcgtccttgc cgtggtgctc gcggatgccg ctggaggtgg tcacccgctc accgagcagc
      721 gccttgagct cggtgaggac gctctggtcg aattcgcgcg ctttgttcat gggctcattt
      781 tagccttatc tccccctcga acggcacccg cacgccgagc gcgggtattt ccagcgtcac
      841 gcgcgcggcg agcgggccct cggccttcag cttgccgtcg gcgagcgcct gctgcaccgc
      901 ctgctcgatc tcgcgctgcg agttcacgcc caccatcttc aggaacttgc ggatgctgag
      961 gttgaaggtt tcctggtcca ttacgtcctc cccatctggt ctttgagtat gctgccggcc
     1021 aattgtttcc ccagctccac gccccactgg tcgaagctgt tgacgttcca gagcaccccc
     1081 tgcacgaaca ccttgtgctc gtaggcggcg acgagcgcgc cgagggctcg cgcgtcgatg
     1141 cgcgcgaggc gcagggtgct cgaggggcgg ttgcccgggt aacggcggtg cggcggcagc
     1201 cccgggtccg tggtgccgaa ggcaagcgcg cgcgcctgcg cctcggcgtt cgcgtcgagc
     1261 tcggcgctca cgccggcgac gatgaaatcc gcgggcacga cctgcgtgcc ctggtgcagc
     1321 agctggtgga acgaatgctg gctcacggtg ccctcggccc cccacaccac gggcgccgtg
     1381 gcgtagccga gcgcgcggcc ttcgcggtcg acgcgcttgc cgttcgattc catctccagc
     1441 tgctgcaggt aggcggggag gagcttcagc gcgtgcgcgt agggcaacac gcagtgcgtg
     1501 gctgcgccga gaaaattcac gttccaggcg ccgagcagcg caagcagcac cgggacgttc
     1561 ttctcgagcg gtgcttcgag gaagtgccgg tccatgtcgc ggccgccggc gaggaactcg
     1621 tcaaatgtat cgggcccgag cgcggcgagc gcgctgaagc tcgccgccga ccagatcgag
     1681 aagcgcccgc cgacccagtc ccacatcggc agcacctcgg ccgcaccgaa ggcgcgcgcc
     1741 gccgcctcgt tcgcgcttac cgcgaagagc ttgccgcgcc cgccgagcca gtcccttgcc
     1801 gcgcgcgcgt tggccatggt ctccgcggtg gtgaaggtct tcgataccgc caccaccagc
     1861 gtcgtcgccg gctcggcgcc ctccagcgcg cgcgagagat cgagcggatc gatgttcgcg
     1921 gcgaaacgca cgtcgatcct ggcgtgctcg cgcagcgcgt ccatgaggag ccgcgcgccg
     1981 aggtcggagc cgccggtgcc caggtgcacg atgcgcttca cgccgccgag cccggcggcg
     2041 agcgcgcgca tgcgcaagag cgccgctttc acttcactcg gcgccgcgtc gccggcgcgc
     2101 agcgccgcgt ggcgcacggc gcggttctcg gtgacgttga tcggcgcgcc ggcgaagagc
     2161 gcggcgcgcc agccctcgat gccgcgctcg gcggcgagct gtgcgagcag ccgcagcgtg
     2221 agcgcgccga cacgctggcg cgagtaatcg agcagcagcg caggcgcgtg ggcgccgaag
     2281 cgcgcgctgc gctcggcgtc gtggcgaaag agctccgcga ggtgcgtgtc gcgccagcct
     2341 tcggcgtgcg cggcaagctt cgcccaggcg gggcagtcgg tcggtggcgt ggtagtcacg
     2401 ggacgtccag gcggtagagg cggtagcgct cgttcttgtc gccgggccgg ccgacgtcgg
     2461 cgagcttcga ccagcgcgcg tcgggcccgt cgcgctcgtc gcgcggcgag ccctgcacga
     2521 gcagcagccg gcagccatcg gcgccggcgc ggctcgccgg ctcggtgcgc agcgcgccat
     2581 ggtagctgag cgcggcgcgc tgcgacacgc ccaggttttg cccggcgatg cagccggcat
     2641 cggcgggcac gcgcgaccgg atctgcagcg cgaccgggcg atagctcttc tggtagtcgg
     2701 cccagggcat ccacagcatg gcaaagctgc cccagagcag cgtcacgccc gccgcccagc
     2761 gcgtgacgcc gcgcatcggc gagggcgcgg tgaagaacgc gacgtacagc cagccgacga
     2821 gaagcgccag cgcaagcaga aacggcgccg cagcgaactg cgcctcgaaa ccgggcgccg
     2881 acttggcgaa gttgtgcgcg atcttgggcg gcacgccaag catcatggcg acgtagccga
     2941 gccacaccag tgcggcaaag aaggtgaagc acatgaccgc gaaccagtcg agcgccgccg
     3001 cggcgccgcg gcgcagccgc gcgacccctt cggcgccgag cagcgcgagc ggcgcgagca
     3061 gcgcgatcac gctggcatcc tgcggcgggc cggtggcggc gagcgcggcg agggtgagca
     3121 ggcaagcggc cgccggcgcg aacaggcgcg gctcgcgcca ctcgcggcgc cgcgcccaga
     3181 gcgcccacgc ggcgagcggc cacgccggcc aggtgaacca gctgccggtg accaggtagt
     3241 agcgcaggtt gtcgagcggc gtgccctgcc gctgcgtcgc ggccgaccac cagtccgcgg
     3301 acagggcggg cgcgcgcgcg ccgagcgccc agggccagct cgcggcgagc gccgcggcga
     3361 cgagcagcgc cgcggcgagg aacggcagcg cgcgccagct gcgccacgcc ggacatgccg
     3421 cgtgcgccgc agccacggcg aggccgagcg ccgccggcac cagcgggccg agcgcgagaa
     3481 atgcgagccc gagcgcggcg ccgaaggcag caccgtgcag cagcggccgc gcgtgggcct
     3541 gcggcagcaa ggcgtaggcg gcgcaggccg cggccagcgc ggcgagctcg ggcagcgcct
     3601 cgtgcgcgtg caccatgaag ccgatcgagc ccagcaccag cagcggcgcg gcggcgccgg
     3661 cggtgcggta gagcagccac agcgcaacga gcacgcaggc gccgctcgcc agccgcgccg
     3721 cgctgtggaa cggcaccgcc cagccggcga gcttgccgaa ggcgagcgcg atccagtgat
     3781 agagcggcgg gtcctcgagc cagggctcgc cggcgaggcg cggcaccacc cagtcgcccg
     3841 agacgtgcat ctgatacgcg atctcgatgg caatggcgtc gtacgccttc cagggatcgt
     3901 gaccagtgag tcccggcagc acgtaggcga gcgcgatcag cgccagcacc gcgggcgcgg
     3961 gcggcagtgc gaggcgggga atcatgacgc gattctaggc ccggaaaaca aaaaggcagc
     4021 cgcagctgcc ttcccggggg tgcgcgaacg ccgcctcagg cggacttctt cgccttggtg
     4081 tagcggcgct tgaatttctc cacgcgtccg gcggtgtcca cgatcttctg cttgccggtg
     4141 tagaacgaat gcgattccga ggacacctcg accttgacca ccgggtaatc gttgccgtcg
     4201 gaccatttca tggtctcgcg gcccttggtg tcgatcgcgg agcgcgagag gaagctgtgg
     4261 ttcgagctgg tgtcgaggaa gatcaccggc tggtaatcgg gatggcctgt cgtcttcatg
     4321 gcggggaatg ggctgcggaa aagcggcgaa ctatacagaa atcagctact tgggtcaacg
     4381 ccggcggcgc cgctacagcc ggccctggcg cttgagctcg aggtagcggt tgacgagcga
     4441 gatcgcgagc cgctcgggct cgacgtccag gcacaaagcg cccgccgcgg cgacgcggcc
     4501 gaaggcgcgc tcgcgctgcg ccaggtagtc ggcggctgcc gcgtgcgtca ccgcgcggtc
     4561 gaagctgtcc acgcgcgcag cgagcgcgcg cccgaggatc gcctcccgga gcgaggccag
     4621 caccacgagg tggcgcttgc ggagcagccc gagcgcgggg gtgagcgtgt cgtcgtcctc
     4681 gtcgcgcagg ttggtgagca gcaccaccag agcgcgccgg cgcattcgcg ccatcacctc
     4741 gcgcgcagcg cgcaggtagt cgggcattgc cagcgagggc tcgaggtcgt acaggcgctc
     4801 gaggatggtg ttcaccgtgg ccgccgactt cctcggcgcg acgtagcgcg gcgcgccgga
     4861 cgcgcccagg gtgagcacac cgacggcgtc gccctggcgc agcgccacgt gcgcgagcag
     4921 cagcgcggcg ttcagggcat ggtcgaagtg cgacagcgcg tcgtccttgg cggccatgcg
     4981 ccggccgcag tcgatcacca gcagcacgcg ctggtcgcgc tcgtcctcgt actcgcgcgc
     5041 gatcaggcgc gcggtgcgcg cggtggcctt ccagtcgatg gcgcgctggc tgtcgccctg
     5101 gcggtactcg cgcagctggt ggaactccat gccctcgccg cggcggcgca cctgcagcac
     5161 gccgatctgc gacaggcggt tgtcggtggc gagcagcgtg tagcgcgcaa gggcgcggaa
     5221 gttcgggaac acgcgcgctt gggcggcttc acccgcgcgc cgcgccacct gccacaggcc
     5281 gagcggcgag gcgaggcgca tctccgtttc gccgaaggcc acggcgccgc gcgcgagcgg
     5341 ccgcgcgcgg tagcgcactt cgcgccactc gcccggcgcc agcgccacgg tctgcggcag
     5401 gccctcgcac tcgaagctcg ccgggtgcag gtcgtttacc tccaggcgca gcgcgcgcgc
     5461 cgaggcgttg gcggcgcgca gcgcgacctc gaagggcacg ccgagggcga gggaatgcgg
     5521 cacgcgccgc gccacgccag gcgcgggcag gcgcagcccg gcggccgcgt cgagcgcggc
     5581 ggcgagcgcc gccgccccgg cgacgagcgt ccaggccggc ccaagcgcgg gcgcgagcgc
     5641 cgcgagtacg ccgagcgcga agagcagcgc ggcggcccac agcaggcgcg gggccggcac
     5701 cagcggaaag ggccgcgcgg cgctcactgc cgcggcgctt gcgtcttgtc cagaatgtcc
     5761 gtcagcaccg cgtccgcgtc gcgcccttcg agctcgagct cgggcg
//
LOCUS       DBNHKE010000242        17328 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_100502, whole genome shotgun sequence.
ACCESSION   DBNHKE010000242 DBNHKE010000000
VERSION     DBNHKE010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_100502"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="AC16O9_16510"
     CDS             <1..967
                     /locus_tag="AC16O9_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6518257.1"
                     /translation="ETIPGIEAQIPLEWLMKQVELTLTEEEKKQIQALGGWEKLMETL
                     KQRLEEQKGRHQGGSKWIGTGGTSPFGAYGYNPEGIRIGQDRSRNRSAVKVWDQREYR
                     NLDDQVELGTRNIKVALRRLRRFAREGAPEEFDLETTIDSTARKAYLDIHMRPERHNT
                     VKVLMFMDIGGTMDDHIKLAEELFSASKTEFKHLEFYYFHNCLYDFVWKDNRRRHSER
                     TRTWDILHKYGHDYKVIFVGDATMSPYEILQPGGSIEYFNEEPGAVWVKRVSEVYSKC
                     VWLNPEPEEIWGYRQSIQIMKELMNGRMYPTTLEGLERAMRQLAK"
     gene            964..1992
                     /locus_tag="AC16O9_16515"
     CDS             964..1992
                     /locus_tag="AC16O9_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518258.1"
                     /translation="MSLLFPTTLVGSYPQPEWLLDRSKLLSRLPPRVRAKELWKIPPE
                     ALAEAQDDATIVAIKAQEEAGLDIITDGEIRRESYSNRFATALEGVDIDNPGTAMDRA
                     GKPVPVPRIAGRIRRKHPVEVEDLKFLKRHTTRKVKMTVPGPFTMMQQAQNDHYKSDE
                     DAAMDYAVAVNEEIKDLFAAGADIVQVDEPYMQARPEKAREYGLKVLNRALDGITGTT
                     AVHICFGYAAVVHKRPSGYSFLPEFALCRCQQVSIETAQSNLDCSVLAKLAGKSIMVG
                     CIDLNDMTVETPQQIAARIERALPYVDKTHVILAPDCGMKYLPRTVADGKLRAMVDAA
                     RLLRAEYA"
     gene            1994..2797
                     /locus_tag="AC16O9_16520"
     CDS             1994..2797
                     /locus_tag="AC16O9_16520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6518259.1"
                     /translation="MAGRLSRLVLNLRRNIPPGTPLDRHLQRFARVAGWFGAEMDLLA
                     ALYGTDKFGEHDYTPVYEELMRASRRKAVRLLEVGVGGYAGALGGESLRMWASYFRRG
                     RITGIDLYDKTALSGGRVTVYQCSQVDAPKLTSLAKELGPFDFILDDGSHMNAHQIET
                     FGILWPHLKDGGTYIVEDVQTSYWPSYGGGPLGTPGYEKSCMQWFKALADSVNIPEFL
                     QPAPGGLDAGIASIAFHHNLIVLKKDLAERYSNARLDDPVVKERLMAPG"
     gene            complement(2794..5742)
                     /locus_tag="AC16O9_16525"
     CDS             complement(2794..5742)
                     /locus_tag="AC16O9_16525"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR6518260.1"
                     /translation="MDWTLLIEALLLVAAAGIIFAAWMHTRQRDRLAARALRASEERF
                     RSLTALSADWFWETDAEHRITWLTGGSTVLAFFGSGLAFGKRLWELPGVELDGRAFTA
                     HLEQLRRHAPFFELELSRTHASGSRHVHVVSGQARYDAAGAFLGYRGVGRDVTERRRG
                     ERELAAAKERLQLALDGSNASLWDTDLQSGKVYLSEGWTEFLGLPRAAAAATTTLEEL
                     MHGVHPDDLERAVKLSVEVMKGERQDYAEEHRFQVADGSWKWLLSRGRITERDADGRA
                     LRMTGINLDITARKHAEEAMREAEERYRSLIELAPDAVIVSSGGVIEYANPAAARLLK
                     AAAPKVLLGRGMEAFVHPEHVERYRERLRYLGAGPGATGFEERRLRCADGSEALVEIA
                     SVSFLERGRLVVQTVARDVTEQRDARRVLAERERRFSDVVEASGEYVWETDAQWRYTY
                     LSKRVESVLGYMRAELLGRTPREFMPLGEPRALDEWFGRSTREGQPFRDLVHRSITKS
                     GKVIWQQVSGIPVRDAAGMLTGYRGTGADVTARKQAEERIQYLATRDALTGLPNRALL
                     GDRAAQSILAAARSRGMLAVLLIDLDRFKLVNDSLGHRAGDALLHAVAERFSNTLRRD
                     DTLARLGGDEFVLLWDGLKAAQDAALVAQRVQAILQRPFTLEGRALNVTASIGIAIYP
                     EDGRDFNELLKNADTALYDAKEAGRSVFRFFSPELHTRAVERLALESDLRGALARSEL
                     QLRWQPVVRHDAVVGAEVLLRWNHPQRGLLKPDAFIPQAEECGLIRAIGEWTLERALS
                     QIGAWQRQLPGAPWFALNVSALELAQGEAYVARLRDALAAHGVPAERLELEVTERVLM
                     AHLPESVETLRRIGALGVRLAIDDFGTGYSSLAYLRRLPIHKLKIDRGFLRELESSAG
                     DAMIIETVAAMAKGLGLLVAAEGVETDAQLRRLRAVGCEEWQGHLYSEPLEAPEFERL
                     ARSGRAAKSA"
     gene            complement(5744..7546)
                     /gene="tfpB"
                     /locus_tag="AC16O9_16530"
     CDS             complement(5744..7546)
                     /gene="tfpB"
                     /locus_tag="AC16O9_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly ATPase TfpB"
                     /protein_id="HIR6518261.1"
                     /translation="MAQAATASAAERRLTLLELIDWLVEDGAVARGPGEQLKKERRYW
                     RGAQHPLAMIAAEKWKDARAPHKPLTLDALAEWLAQRVGMEYLHIDPLKIDFSAVTEV
                     MSSAYASRFRILPVSLTAKEAVIATAEPYVREWEPEIARVIQRPIKRVIANPQDIERY
                     QVEFYNLARSVKGANRGAAGASGLSNFEQLVELGSANKSLDANDAHIVRVVDWLWQYA
                     FEQRASDIHIEPRREVGLVRFRIDGVLHQVYQIPASVLAAMTSRIKILGRMDVVEKRR
                     PQDGRIKTRMADGDEVELRLSTLPTAFGEKLVMRIFDPEVLVRDFRELGFSEEDHRRW
                     KEMTARPNGIVLVTGPTGSGKTTTLYSTLKSLATPEVNVCTIEDPIEMVEPAFNQMQV
                     QQAIDLGFAEGVRALMRQDPDIIMVGEIRDLETAEMAVQSALTGHLVLSTLHTNDAPS
                     AVTRLLELGIAPYLLNATFNGVMGQRLLRTLCPHCKQPVELNRAEDQAAWDALVAPFT
                     SKRPTTVYKPVGCLECRMTGFMGRVGIYETLLHTPPIKQMITAGADLAAVREQAMKDG
                     MKPLRVSGAMKVAAGLTTIDEVLKVAPPVRENTA"
     gene            7578..8855
                     /locus_tag="AC16O9_16535"
     CDS             7578..8855
                     /locus_tag="AC16O9_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518262.1"
                     /translation="MPKAKALAPEVKLREVWAWSMYDFANSGYTTVVITAVYNAFFVA
                     AVAGRAPWATFAWTAALSVSYLAVLLTAPLVGAWADAHAAKKRLLFYATVGCVLFTAA
                     LYFVGPGAVALAIVLIVLSNFFFATGENLIAAFLPELADSRSMGRVSGWGWAFGYLGG
                     LVSLGLSLIYITWAQGAGQSGTQFVPVVMLITAAVFALCATPTFLFLKERAVPQMRDG
                     KAWARVMHTLHRAQQYRDLRRFLVCILFYQAGIMAVIALAAIYAQEAMKFTQQQTIVL
                     VLVVNVTAALGAFGFGYLQDAIGHVRAMALILAGWIGMITLAYFAEDAATFWVAANLA
                     GLCMGSSQAAGRAIVGYLSPRTREAEFFGLWGLAVKAASIFGPLTYGLVTWIFAGDHR
                     LGILAVGAYFVIGLVLLAGIDVERGRRAALAPA"
     gene            complement(8860..9282)
                     /locus_tag="AC16O9_16540"
     CDS             complement(8860..9282)
                     /locus_tag="AC16O9_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6518263.1"
                     /translation="MQRNGSAVWKGGLKDGSGQVSTGSGGLASAPYTFASRFESGGKT
                     DPEELVAAAHAACFSMALSGQLGQANLRADSIATTASLTLDKTDAGMTVTAIHLVVKA
                     KVPGADKAVFEKAAGNAKAGCPISRLLNAKITLEATLE"
     gene            9362..9871
                     /locus_tag="AC16O9_16545"
     CDS             9362..9871
                     /locus_tag="AC16O9_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR6518264.1"
                     /translation="MSIKVGDRVPDGVLFESIGYSDENHCPARPGQVKVAELVQGKRI
                     AIFGLPGAFTMTCSGKHLPGYLANYDKFKAKKVDEIWCVSVNDGYVMAAWGRDQKADG
                     KIRMLGDGNGQWTKALGLEVDSSAGGMGMRMRRCSMLVEDGVVRQLNLEAPGKFEVSD
                     AETLLKQLG"
     gene            9868..10185
                     /locus_tag="AC16O9_16550"
     CDS             9868..10185
                     /locus_tag="AC16O9_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518265.1"
                     /translation="MTRAALLGVLLLATATVHAQSRTEAGRQQFREASRICRYVPHAE
                     QRACMARELCKNNREPAACEERYFVNAERRDIVLEACKGKQGTVLRDCMREEYKKLPE
                     PRS"
     gene            10202..12877
                     /locus_tag="AC16O9_16555"
     CDS             10202..12877
                     /locus_tag="AC16O9_16555"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6518266.1"
                     /translation="MKKLLACLLLAALPALAAPQKVTEVEGVAEYRLDNGLRVLLAPD
                     AGAETVTAHVTYLVGSRHEGYGEKGMAHLLEHMLFKGSTNHPDVKQEFAKRGARWNGT
                     TSYDRTNYFETLPASGDNLEWALAMEADRMVNSFVRKADLESEMTVVRNEFEMGENSP
                     GSVLFQRMLRLAFNWHNYGHAIIGARSDIESVPIERLQAFYRTWYQPDNALLTLGGRF
                     EAARALALVEKHFGAIPRPARKLPELYTVEPTQDGERSVTLQRTGDTPLVAALYRVPA
                     ASHPDFPAIEVLVEAMRAAPQGRLHRALVQKGLASAIWGFEHALHDPGYIAFGAQLAK
                     DGDLEAAGAALLDTLHAAPKAPIEADEVERGRTTLLNEMDKATLDSGALVRALAEFHA
                     LGDWRLYFLYRDRLRAVKAPDVQRVAETYLRPANRVLGRFVPTENPQRAQIPEAPDLQ
                     AALQGYRGGAALAAGEAFDPAPQNIEARLVRRTLANDVRLALLPKRTRGQKVVIKLDL
                     HWGNEQNTLGRSTACSLAGEMLMRGTKRHTRAELREAFDRLQATVRISASGATLETRR
                     EHLAEALRLVAEVLREPAFPDAEFEELKRSTLTSAQAELSDPAAIASQQLRRHLHPYP
                     KGHWHYEQSPEERVAALEAATLEEARACHAQLIGGTGADVAAVGDFEAAELAALVELL
                     FGDWKNPVPYAHIPKRYFEHPALERAFVTPDKANAVLRAGLNLRLRDDHPDFPALVLA
                     NYLLGGSSTARLPERIREKEGLSYSTYTWFMASPLDPVASFSVSAIFAPQNKARVERA
                     VREELERALREGFAEEEVAAAKRGLLESRRLARTQDGALVARLAFYLHLNRTFAWDID
                     FERRIAELEPARLREALARHVDLARLAVTMAGDFK"
     gene            complement(12882..13268)
                     /gene="mscL"
                     /locus_tag="AC16O9_16560"
     CDS             complement(12882..13268)
                     /gene="mscL"
                     /locus_tag="AC16O9_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR6518267.1"
                     /translation="MSMLQEFKSFAMKGNVVDLAVGIVIGAAFGKIVSSLVGDVIMPV
                     VGSIVGGIDFSGLAVNIGDASVKYGKFIQSTVDFVIIAAAIFLVVKAINRMKREEAKP
                     AAPAAPPAPPREQVLLEEIRDLLKSR"
     gene            complement(13284..13832)
                     /gene="greB"
                     /locus_tag="AC16O9_16565"
     CDS             complement(13284..13832)
                     /gene="greB"
                     /locus_tag="AC16O9_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR6518268.1"
                     /translation="MSKAFVRESDGAGEEDERADAPVPAKNYITPAGFARLDAERRRL
                     VERERPEVVRAVSAAAANGDRSENADYIYGKKRLREIDRRVRFLIKRLENASVVDNAG
                     RDTEQVFFGATVRVALRTGEQRSITIVGADEVDPARGRVSWLSPIARALLKARAGDVV
                     RLRTPVGVEELEIVGLVYDPID"
     gene            13895..14608
                     /locus_tag="AC16O9_16570"
     CDS             13895..14608
                     /locus_tag="AC16O9_16570"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR6518269.1"
                     /translation="MAFQRKVDRDDLLTRLRVREVALFSLIALTAILANLPESYARQL
                     GIQHDYLYAALVGAVVIGLFLYLKFFFFFAVVLLILGANLPEQIAQDFGISKIPLILA
                     LVAMVGVSLINYVVKVLPTGLEPKPREKSPEGVRALLYAIDKNNVVYGQRVLAMNFDP
                     NLHHENGYTPLAYAALKGNPQMIEMLLGNGADPGLTTREGETPVELALRAGHTGLADL
                     LKQARQARETALAAGSPPG"
     gene            14651..>17328
                     /locus_tag="AC16O9_16575"
     CDS             14651..>17328
                     /locus_tag="AC16O9_16575"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR6518270.1"
                     /translation="MNETPVVPAIAPQAICVDALLEKYAKGGEATVGEVRARVARALA
                     QAEPEARRSHWERRFLQALEDGFIPAGRINSAAGVQLQATLINCFVQPVGDSISEVVD
                     GKPGIYTALQEAAETMRRGGGVGYDFSSIRPKGAEVKGTRSRASGPVSYMRVFDRSCE
                     TVESAGARRGAQMGVLRCDHPDIEDFIHAKDQGDLANFNVSVGVTDAFMQAVEQDAEV
                     ELVHKAKPWPGPDVAQRADGLWVYRKVRARALWDQIMRSTYDHAEPGILFLDRMNRDN
                     NLNYCEAIEATNPCAEQPLPPYGCCCLGSIDLTRFVRNAFAADAAFDYEAFGAVVDVA
                     VRMLDNVLEVTAWPLEAQRREAMAKRRVGLGFTGLGDALIMLRQHYDSDAARAAAARI
                     SAAMRDRAYLASAALAEERGAFPLFNADLYLSGTSFAARLPEEVKAPIRAHGIRNSHL
                     LSIAPTGTISLAFADNASNGIEPPFSWTYTRKKRMPDGTLQEFPVEDHAWRRYKAAGG
                     DMENLPPHFVTALELSAAAHERMVAAVAPFIDTSISKTVNVPEDYPYGEFKDLYLAAW
                     KSGLKGLATYRPNKVLGSVLSVDTPQDFVEDDVNRRIRIKTLPEPVLASLRWPGRPEF
                     AAGNLSWTYMIEHPQHHFAVFVGEAENGGPHAFEVWVNGSEQPRGLGAVAKSLSMDMR
                     AEDPGWLRLKLDTLARTAGDDAFDMSFPPRGEKRRMPSVVSALAQLVRWRVEQLGGLD
                     GEKQADAFASVTPVVDSMFSLKEPKTGADGTMSWTVDVHNPRTGDDFVLGLKEIALPD
                     GTTRPYSMWLSGEYPRALDGLCKLLSLDMRVVDPAWIGMKLKKLLDFPEPLGDFLAFV
                     PGSRKQQSYPSTVSYLARLILHRYAMLGLLDEDGAPL"
BASE COUNT         2709 a         6114 c         5862 g         2643 t
ORIGIN      
        1 cgagacgatc cccggcatcg aggcgcagat tccgctcgag tggctgatga agcaggtcga
       61 gctgacgctc accgaggagg aaaagaagca gatccaggcg ctcggcggct gggagaagct
      121 catggagacg ctcaagcagc ggctcgagga gcagaaaggc cgccaccagg gtggttcgaa
      181 atggatcggc accggtggca cctcgccctt cggcgcctac ggctacaacc cggaaggcat
      241 tcgcatcggc caggaccgca gccgcaaccg cagcgcggtg aaggtctggg accagcgcga
      301 ataccgcaac ctcgacgacc aggtcgagct cggcacgagg aacatcaagg tagcgctgcg
      361 ccgcctgcgg cggttcgccc gcgagggggc acccgaggag ttcgacctcg agaccaccat
      421 cgactcgacc gcgcgcaagg cctacctcga catccacatg cggcccgagc gccacaacac
      481 cgtcaaggtg ctgatgttca tggacatcgg cggcaccatg gacgaccaca tcaagctcgc
      541 cgaggagctg ttctcggcct ccaagaccga gttcaagcac ctcgagttct actacttcca
      601 caactgcctg tacgacttcg tgtggaagga caaccggcgg cgccactccg agcgcacgcg
      661 cacctgggac atcctgcaca agtacggcca cgactacaaa gtgatcttcg tcggggatgc
      721 caccatgagc ccgtacgaga tcctgcagcc gggcggctcg atcgagtact tcaacgagga
      781 gcccggcgcg gtgtgggtga agcgcgtctc cgaggtgtac tcgaagtgcg tgtggctgaa
      841 tcccgagccc gaggagatct ggggctaccg ccagtcgatc cagatcatga aggagctgat
      901 gaacggccgc atgtacccga ccacgctcga ggggctggag cgcgccatgc gtcagctggc
      961 gaaatgagcc tgctgttccc caccacgctg gtcggcagct acccgcagcc cgagtggctg
     1021 ctcgaccgct cgaagctgct gtcgcgcctg ccgccgcgcg tgcgcgctaa agagctgtgg
     1081 aagatcccgc cggaggcgct cgccgaagcg caggacgacg ccaccatcgt cgccatcaag
     1141 gcgcaggagg aggccgggct cgacatcatc accgacggcg agatccggcg cgagagctac
     1201 tccaaccgct tcgccacggc gctcgagggc gtcgacatcg acaaccccgg cacggcgatg
     1261 gaccgcgccg ggaaacccgt gcccgtgccg cgcatcgccg gcaggatccg ccgcaagcac
     1321 ccggtcgagg tggaggacct caagttcctc aagcgccaca ccacgcgcaa ggtgaagatg
     1381 accgtgcccg gtcccttcac catgatgcag caggcgcaga acgatcatta caagagcgac
     1441 gaggacgcgg ccatggacta cgccgtggcg gtgaacgagg agatcaagga tctcttcgcc
     1501 gccggcgccg acatcgtgca agtggacgag ccctacatgc aggcgcgccc ggagaaggcg
     1561 cgggagtacg gactgaaggt cctcaaccgc gcgctggacg gcattaccgg gacgaccgcc
     1621 gtgcacatct gcttcggcta cgcggcggtc gtgcacaagc gtccctcggg ctactccttc
     1681 ctgcccgagt tcgcgctctg ccgctgccag caggtgtcga tcgagaccgc gcagtcgaac
     1741 ctcgactgct cggtgctggc gaagctcgcc ggcaagagca tcatggtcgg ctgcatcgac
     1801 ctgaacgaca tgacggtcga gacgccgcag caaatcgcgg cacgcatcga gcgcgccctg
     1861 ccctacgtgg acaagacgca cgtcatcctc gcgcccgact gcggcatgaa gtatctgccg
     1921 cgtaccgtcg ccgacggcaa gctgcgcgcg atggtggatg cggcgcggct attgcgggca
     1981 gagtacgcat agcatggcgg gccgcctcag ccgcctggtg ctcaacctgc ggcgcaacat
     2041 ccccccgggc acgccgctcg accgccacct gcagcgcttc gcgagggtcg cgggctggtt
     2101 cggtgcggag atggatctcc tcgccgcgct ctacggcacc gacaaattcg gcgagcacga
     2161 ctacaccccg gtgtacgaag agctgatgcg cgcctcgcgg cgcaaggcgg tacggctgct
     2221 ggaagtcggc gtcggcggct acgccggggc gctgggcgga gagtcgctgc gcatgtgggc
     2281 gagctacttt cggcgcggca ggatcaccgg catcgacctg tatgacaaga ctgcgctttc
     2341 gggcgggcgc gtcaccgtct accagtgctc gcaggtcgac gcgccgaagc tcacttcatt
     2401 ggcgaaggaa ctgggaccgt tcgatttcat cctcgacgac ggcagccaca tgaacgcgca
     2461 ccagatcgag accttcggca tcctctggcc gcacctgaag gacggcggca cctacatcgt
     2521 cgaggacgtg cagacctcct actggccatc ctacggcggc gggccgctcg gcacgccggg
     2581 ctacgagaag tcctgcatgc agtggttcaa ggcgctcgcc gacagcgtga acattcccga
     2641 attcctgcag cccgcgcccg gcgggctcga tgcgggcatc gccagcatcg ccttccacca
     2701 caatctgatc gtgctgaaga aggacctcgc cgagcgctat tccaacgccc ggctcgacga
     2761 ccccgtggtg aaggagcggc tgatggcgcc gggctaagcc gacttcgccg cccgcccgct
     2821 ccgcgcgagg cgctcgaatt ccggcgcctc cagcggctcg ctgtacaggt gtccctgcca
     2881 ctcctcgcag cccaccgcgc gcaggcgccg cagctgcgcg tcggtctcca cgccctcggc
     2941 ggccaccagc aggccgaggc ccttcgccat cgcggccacg gtctcgatga tcatcgcgtc
     3001 gccggcgctc gattcgagct cgcgcaggaa gccgcggtcg atcttcagct tgtggatcgg
     3061 caggcgccgc aggtaggcga ggctcgaata cccggtaccg aagtcgtcga tcgccaggcg
     3121 cacgcccagc gcgccgatgc gccgcagcgt ctccacggac tccggcaggt gcgccatcag
     3181 cacgcgctcg gtgacttcga gctcgaggcg ctccgccggc acgccgtgcg cagcgagcgc
     3241 atcgcgcagc cgcgcgacgt acgcttcgcc ctgcgccagc tccagcgccg agacgttcag
     3301 cgcgaaccac ggcgcgcccg gcagctgccg ctgccaggcg ccgatctgcg acagcgcgcg
     3361 ctccagcgtc cattcgccga tggcgcggat caggccgcac tcctcggcct gcgggatgaa
     3421 ggcgtccggc ttgagcagcc cgcgctgcgg atggttccag cgcagcagca cctcggcgcc
     3481 gaccaccgcg tcgtgccgga ccacgggctg ccagcgcagc tgcagctcgc tgcgcgccag
     3541 cgccccgcgc aggtcgctct ccagggcgag gcgctccacc gcgcgcgtgt gcagttcggg
     3601 cgaaaagaag cggaaaacgc tgcgccccgc ctccttggcg tcgtaaagcg cggtatcggc
     3661 gttcttcagc agctcgttga agtcgcgccc gtcctcgggg tagatggcga tgccgatcga
     3721 ggcggtgacg ttgagcgcgc gcccctcgag cgtgaacggg cgctgcagaa tcgcctgcac
     3781 gcgctgcgcc accagcgcgg cgtcctgggc cgccttcagc ccgtcccaca gcagcacgaa
     3841 ctcgtcgccg cccaggcgcg cgagcgtgtc gtcgcgccgc agcgtgttcg aaaagcgctc
     3901 ggccaccgcg tgcagcagcg cgtcgccggc gcggtggccg agcgagtcgt tcaccagctt
     3961 gaagcggtcg agatcgatga gcaataccgc gagcatgccg cggctgcgtg ccgcggcgag
     4021 gatcgactgc gcggcgcggt cgccgagaag cgcccggttg ggtagcccgg tgagcgcatc
     4081 gcgcgtggcg aggtactgga tgcgctcctc ggcctgcttg cgcgccgtga cgtcggcgcc
     4141 ggtgccgcgg tagcccgtca gcatcccggc tgcatcgcgc accggtatcc cgctcacctg
     4201 ctgccagatc accttgccgg acttggtgat cgagcggtgc accaggtcgc ggaacggctg
     4261 tccctcgcgc gtgctgcgcc cgaaccactc gtccagcgcg cgcggctcgc cgagcggcat
     4321 gaattcgcgc ggcgtgcgcc cgagcagctc tgcgcgcatg tagcccagaa cgctttccac
     4381 ccgcttcgaa aggtaggtgt agcgccactg cgcgtcggtc tcccagacgt attcgcccga
     4441 ggcctcgacc acgtcgctga agcgccgttc gcgctcggcg agcacgcggc gcgcgtcacg
     4501 ctgctcggtg acgtcgcgcg ccaccgtctg caccaccagc cggccgcgct cgaggaacga
     4561 gacgctcgcg atctccacca gagcctcgct gccatcggcg cagcgcagcc tgcgttcctc
     4621 gaagccggtg gcgccgggcc cggcgccgag gtagcgcagc cgctcgcggt agcgctcgac
     4681 atgctccggg tggacgaacg cctccatgcc gcggcccagc agcaccttgg gcgccgcggc
     4741 cttcagcagg cgcgccgccg ccgggttggc gtactcgatg acgccgccgc tcgacacgat
     4801 cacggcgtcg ggcgcgagct cgatcaggga gcggtagcgc tcctcggctt cgcgcatcgc
     4861 ctcctcggcg tgcttgcgcg cggtgatgtc caggttgatg ccggtcatgc gcaatgcccg
     4921 cccgtcggca tcgcgctcgg tgatgcggcc gcgcgacagg agccacttcc agctgccgtc
     4981 ggcgacctgg aagcggtgct cctcggcgta gtcctggcgc tcgcccttca tcacctcgac
     5041 cgacaacttc accgcgcgct ccaggtcgtc cggatgcacc ccgtgcatca gctcttccag
     5101 ggtggtggtg gcggcggcgg ccgcgcgcgg caggccgagg aactcggtcc agccctcgct
     5161 caggtagacc ttgccgctct gcaggtcggt gtcccagagg ctggcgttgg agccatcgag
     5221 cgccaactgc aggcgctcct tggcggcggc gagctcgcgc tcgccgcgcc ggcgctcggt
     5281 gacgtcacgg cccaccccgc ggtagccgag gaaggcgccg gcggcgtcat agcgcgcctg
     5341 gcccgaaacc acgtgcacat ggcgcgagcc gctggcgtgg gtgcgcgaga gctcaagctc
     5401 gaaaaacggg gcgtggcggc gcagctgctc gaggtgcgcg gtgaaggcgc gcccgtcgag
     5461 ctcgaccccg ggcagctccc acaggcgctt gccgaacgcg agccccgagc cgaagaaggc
     5521 gagcacggtg gagccgcccg taagccaggt aatgcggtgc tcggcgtcgg tctcccagaa
     5581 ccagtcggcc gacaacgccg tcaggctgcg aaagcgctcc tcgctcgcgc gcagcgcacg
     5641 ggcggcgagg cggtcgcgct gccgcgtgtg catccaggcg gcgaagatga tgccggccgc
     5701 cgcgaccagc agcagcgcct cgatgagcag cgtccagtcc atgctaggcc gtgttctcgc
     5761 gcaccggcgg cgccaccttg agcacctcgt cgatcgtggt gagtccggcg gcaaccttca
     5821 tggcgccgct cacgcgcagc ggcttcatgc cgtccttcat cgcctgctcg cgcaccgcgg
     5881 cgaggtccgc gcccgcggta atcatctgct tgatcggcgg cgtatgcagc agcgtctcgt
     5941 agatgccgac gcggcccatg aagccggtca tgcggcactc caggcagccc acgggcttgt
     6001 acaccgtcgt cggccgcttc gacgtgaacg gcgcgacgag cgcgtcccag gccgcctggt
     6061 cctcggcgcg gttgagctcc accggctgct tgcagtgcgg gcagagcgtc cgcagcagcc
     6121 gctgccccat gacgccgttg aacgtcgcgt tcagcaggta gggcgcgatg ccgagctcga
     6181 gcagccgggt caccgcgctg ggcgcgtcgt tggtgtgcag cgtcgagagc acgaggtgcc
     6241 cggtcagcgc cgactgcacc gccatctcgg cggtctcgag atcgcggatc tcgcccacca
     6301 tgatgatgtc cgggtcctgg cgcatgagcg cgcgcacccc ttcggcgaag ccgaggtcga
     6361 tcgcctgctg cacctgcatc tggttgaacg ccggctcgac catctcgatc gggtcttcga
     6421 tcgtgcacac gttcacctcg ggcgtcgcga ggctcttcag cgtggaatac agcgtcgtgg
     6481 tcttgcccga gcccgtcggt ccggtcacca gcacgatccc gttcgggcgc gccgtcatct
     6541 ccttccagcg gcggtggtcc tcctcggaga agccgagctc gcgaaagtcg cgcaccagca
     6601 cttcggggtc gaagatgcgc atcaccagct tctcgccgaa cgcggtgggc agcgtcgaca
     6661 ggcgcagctc gacctcgtcg ccgtcggcca tgcgcgtctt gatgcgcccg tcctgcgggc
     6721 ggcgcttctc caccacgtcc atgcgcccga ggatcttgat gcggctggtc atcgccgcca
     6781 gcaccgaggc cgggatctgg tacacctggt gcagcacgcc gtcgatgcga aagcgcacca
     6841 ggcccacttc gcgccgcggc tcgatgtgga tgtcgctcgc gcgctgctcg aaggcgtact
     6901 gccagagcca gtcgacgacg cgcacgatgt gcgcgtcgtt cgcatccagc gacttgttgg
     6961 cgctgccgag ctcgaccagc tgctcgaagt tggaaagtcc cgaggcgccc gcggcgccgc
     7021 ggttggcgcc cttcaccgag cgcgccaggt tgtagaactc gacctggtag cgctcgatgt
     7081 cctgcgggtt ggcgatcacg cgcttgatcg gccgctggat gacgcgcgcg atctccggct
     7141 cccactcgcg cacgtagggc tcggcggtcg cgatgaccgc ttccttggcg gtgagggaga
     7201 ccggcagtat gcggaagcgc gaggcgtagg cgctcgacat cacctcggtg acggccgaga
     7261 agtcgatctt cagcggatcg atgtgcaggt attccatgcc cacgcgctgc gccagccact
     7321 cggcgagcgc gtccagggtg agcggcttgt gcggcgcgcg cgcgtccttc cacttctcgg
     7381 cggcgatcat ggcgagcggg tgctgcgccc cgcgccagta gcgccgctcc ttcttcagct
     7441 gctcgccggg cccgcgggcg acggccccgt cctcgaccag ccagtcgatg agctcaagta
     7501 gcgtcagccg gcgttcggcg gcggacgcgg tggcggcctg tgccatgggc gcggacacta
     7561 taccgctaga atccggtatg cccaaggcaa aggcgctggc gcccgaggtc aaactgcgtg
     7621 aagtctgggc gtggagcatg tacgacttcg ccaactcggg ctacaccacg gtggtcatca
     7681 ccgccgtcta caacgccttc ttcgtcgccg cggtggccgg gcgcgcgcct tgggccacct
     7741 tcgcctggac ggcggcgctc tcggtgtcct acctcgccgt gctgctcacg gcgccgctgg
     7801 tcggcgcctg ggccgatgcg catgcggcga agaagcgttt gctgttctac gcgacggtcg
     7861 gctgcgtgct gttcaccgcg gcgctgtact ttgtcggacc cggcgcggtg gcgctggcca
     7921 tcgtgctcat cgtgctgtcg aacttcttct tcgccaccgg cgagaacctg atcgccgcgt
     7981 tcctgccgga gctcgccgac tcgcgctcca tggggcgcgt ctccgggtgg ggatgggcct
     8041 tcggctacct cggcggcctg gtctcgcttg gcctgtcgct catctacatc acctgggcgc
     8101 agggcgcagg ccagagcggc acgcagttcg tgccggtcgt catgctgatc accgcggccg
     8161 tattcgcgct gtgcgcgacg cccacgttcc tgttcctgaa ggagcgggcc gtgccgcaga
     8221 tgcgcgacgg caaggcatgg gcgcgggtca tgcacacgct gcaccgcgcg cagcagtacc
     8281 gcgacctgcg gcgcttcctc gtctgcatcc tgttttacca ggcgggcatc atggcggtga
     8341 tcgcgctcgc cgcgatctac gcgcaggagg cgatgaagtt cacgcagcag cagaccatcg
     8401 tgctcgtgct ggtggtcaac gtcacggccg cgctcggcgc cttcggcttc gggtacctgc
     8461 aggacgcgat cgggcacgtg cgcgcgatgg cgctcattct cgccggctgg atcggcatga
     8521 tcaccctcgc ctacttcgcc gaggacgccg cgacgttctg ggtcgcggcc aacctcgccg
     8581 gcctgtgcat gggctcctcc caggcggccg ggcgcgccat cgtcggctat ctttccccgc
     8641 gcacgcgcga ggcggagttc ttcggcctgt gggggctcgc ggtgaaggcc gcctcgatct
     8701 tcggcccgct cacctacggc ctggtgacct ggatcttcgc cggcgaccac cggctcggca
     8761 tcctcgccgt gggggcgtat ttcgtgatcg gcctcgtgct gctggccggc atcgacgtcg
     8821 agcgcggccg gcgcgcggcg ctcgcgcccg cctgagccgc tactcgagcg tcgcctccag
     8881 ggtgatcttg gcgttcagca gccgcgaaat cgggcagccc gccttggcgt tgccggcggc
     8941 cttttcgaag accgccttgt cggcgccggg gacctttgcc ttcaccacca ggtggatcgc
     9001 ggtcaccgtc atgccggcgt cggtcttgtc gagggtcagg ctcgccgtcg tcgcgatgct
     9061 gtccgccctg aggttcgcct ggccgagctg cccggagagc gccatcgaga agcaggcggc
     9121 gtgcgccgcg gcgaccaact cctcgggatc ggtcttgccg cccgactcga accggctggc
     9181 gaaggtatag ggcgcggagg ccagcccgcc gctgcctgtg gagacctggc cgctgccgtc
     9241 cttcaggccg cctttccaga ctgctgagcc gttacgttgc atgcgagcct ccttggaaaa
     9301 agtgaactca ggcgcgttat tatcgtcgca gtgcttacat tcccactaga aggaaactgc
     9361 gatgagcatc aaagtcggtg atcgcgtgcc ggacggggtg ctgttcgaat ccatcggtta
     9421 cagcgacgag aatcactgcc cggcgcgccc cggccaggtc aaggtcgcgg agctggtgca
     9481 gggcaagcgc atcgcgatct ttggactccc gggcgcgttc acgatgacct gctccggaaa
     9541 gcacctcccc gggtatctcg ccaattacga caagttcaag gcgaagaagg tcgacgagat
     9601 ctggtgcgtt tcggtcaacg acggctatgt catggcagca tggggtcgcg atcagaaggc
     9661 cgacggcaag atccgcatgc tgggcgacgg caacggccag tggacgaagg cgctgggact
     9721 ggaagtggac tccagcgccg gcggcatggg catgagaatg cggcgctgct ccatgctggt
     9781 cgaagacggc gtcgtcaggc agctcaacct cgaggcgccc gggaagttcg aggtaagcga
     9841 cgccgagacg ctcctgaagc agctgggatg acgcgcgccg ccctcctcgg cgtgctgctg
     9901 ctcgccaccg ccaccgtgca cgcgcaatcc cgcaccgagg ccgggcgcca gcagttccgc
     9961 gaggcctcgc gcatctgccg ctacgtgccg cacgccgagc agcgcgcctg catggcgcgc
    10021 gagctgtgca agaacaaccg cgagccggcg gcctgcgagg agcgctactt cgtcaacgcc
    10081 gagcgccgcg acatcgtgct cgaggcctgc aagggcaagc agggcacggt gctgcgcgac
    10141 tgcatgcgcg aggaatacaa gaagcttccc gagccccggt cgtagcgatc cggccgcgcc
    10201 gatgaagaag ctgctcgcct gcctgctgct cgccgccctg cccgcgctcg ccgcgccgca
    10261 gaaggtcacc gaggtcgagg gcgtcgccga gtaccggctc gacaacggcc tgcgcgtgct
    10321 gctggcgccc gacgcgggcg cggagacggt cacggcgcac gtcacctacc tggtgggctc
    10381 gcgccacgag ggctacggcg agaagggcat ggcgcacctg ctcgagcaca tgctgttcaa
    10441 gggctccacg aaccatcccg acgtgaagca ggagttcgcc aagcgcggcg cgcgctggaa
    10501 cggcaccacc tcctacgacc gcaccaacta cttcgagacg ctgccggcct cgggcgacaa
    10561 cctggaatgg gcgctggcca tggaagccga ccgcatggtg aactccttcg tgcgcaaggc
    10621 ggacctcgag agcgagatga ccgtggtgcg caacgagttc gagatgggcg agaacagccc
    10681 cggcagcgtg ctgttccagc gcatgctgcg gctcgccttc aattggcaca actatggcca
    10741 cgccatcatc ggcgcgcgct ccgacatcga atcggtgccg atcgagcgtc tgcaggcgtt
    10801 ctaccgcacc tggtaccagc cggacaatgc actgctcacc ctcggcgggc gcttcgaggc
    10861 ggcgcgcgcg ctcgcgctgg tcgagaagca cttcggcgcc atcccgcgcc cggcgcgcaa
    10921 gctgcccgag ctctacacgg tggagccgac gcaggacggc gagcgttcgg tcacgctgca
    10981 gcgcacgggc gacacgccgc tcgtcgccgc gctgtaccgc gtgcccgccg ccagccaccc
    11041 ggatttcccg gcgatcgagg tcctggtgga ggcgatgcgc gccgcgccgc agggccggct
    11101 gcaccgggcg ctggtgcaga aaggcctggc gagcgccatc tggggattcg agcacgcgtt
    11161 gcacgatccc ggctacatcg ccttcggcgc ccagctcgcc aaggacggcg acctcgaggc
    11221 ggcgggcgcg gcgctgctcg acacgctgca cgcggcgccg aaggctccga tcgaggccga
    11281 cgaggtggag cgcgggcgca ccacgctgct gaacgagatg gacaaggcga cgctcgactc
    11341 gggcgcgctg gtgcgcgcgc ttgccgagtt ccacgccctg ggcgactggc gcttgtactt
    11401 cctgtaccgc gaccgcctgc gcgccgtgaa agcgcccgac gtgcagcgcg tggccgaaac
    11461 ctatctcagg ccggccaacc gcgtgctcgg gcgcttcgtg ccgaccgaga acccgcagcg
    11521 cgcgcagatt ccggaagcgc cggacctgca ggcggcgctg cagggctacc gcggcggcgc
    11581 cgcgctcgcc gcgggcgagg ccttcgatcc cgcgccacag aacatcgagg cgcgcctcgt
    11641 gcggcgcacg ctcgccaacg acgtgcgcct cgcgctcctg cccaagcgca cgcgggggca
    11701 gaaggtcgtg atcaagctcg acctgcactg gggcaacgag cagaacacgc tcgggcgcag
    11761 caccgcctgc tcgctcgctg gcgaaatgct gatgcgcggc acgaagcgac atacgcgcgc
    11821 cgagctgcgc gaagcgtttg atcgcctgca ggcgacggtc aggataagcg cgagcggcgc
    11881 cacgctggag acgcggcgcg agcacctcgc cgaggcgctg cgcctggtgg ccgaggtgct
    11941 gcgcgagccg gcgtttccgg acgctgagtt cgaggagctc aagcgcagca cgctcaccag
    12001 cgcgcaagcc gagttgagcg acccggcggc catcgcctcg cagcagctgc gccgccacct
    12061 gcatccgtac ccgaagggcc actggcacta cgagcagtcg cccgaggaac gcgtggcggc
    12121 gctcgaggcg gcgacgctgg aagaggcgcg cgcttgccac gcgcagctca tcggcggcac
    12181 cggcgccgac gtggcagcgg tgggcgactt cgaggccgcc gagctcgcgg cgctcgtgga
    12241 gctgctgttt ggcgactgga agaatcccgt gccctacgcg cacatcccga agcgctactt
    12301 cgagcacccc gcgctcgagc gcgcgttcgt cacgcctgac aaggcgaacg cggtgctgcg
    12361 cgccgggctc aatctgcgcc tgcgcgacga ccatccggac ttccccgcgc tggtgctcgc
    12421 caactacctg ctcggcggct cctcgactgc gcgcctgccc gagcgcattc gcgagaagga
    12481 agggctgtcc tacagcacct acacctggtt catggcgagc ccgctcgatc cggtggccag
    12541 cttctcggtc tcggcgatct tcgcgccgca aaacaaggcg cgcgtcgagc gcgccgtgcg
    12601 tgaggagctg gagcgcgcgc tgcgcgaggg ctttgccgag gaggaggtgg cggcggcaaa
    12661 gcggggactc cttgaatcca ggcgcttggc gcgcacgcag gacggcgcac tcgtcgcgcg
    12721 tctagcgttc tatctccacc tgaaccgcac gttcgcctgg gacatcgatt tcgagcggcg
    12781 catcgccgag ctcgagccgg cacggctgcg cgaggcgctc gcccgccatg tcgacctggc
    12841 gcggctcgcg gtcaccatgg cgggagattt caagtaggac gctagcgcga cttcagcagg
    12901 tcgcggatct cctccagcag cacctgctcg cgcggcggcg cgggcggggc cgcgggcgca
    12961 gccggcttcg cctcttcacg cttcatgcgg ttgatcgcct tcacgaccag gaagatggcg
    13021 gcggcgatga tgacgaagtc gacggtgctc tggatgaact ttccgtactt cacggaggcg
    13081 tcgccgatat tgaccgccag gcccgaaaag tcgatgccgc cgacgatcga gccgaccacc
    13141 ggcatgatga cgtcgccgac cagtgacgac acgatcttgc cgaaggcggc gccgatcaca
    13201 atgccgacgg cgaggtcgac gacgttgccc ttcatggcga agctcttgaa ctcctgcagc
    13261 atgctcatcg cgctctccct gggtcagtcg attggatcat agacgagtcc gacaatctca
    13321 agctcttcca cgcctaccgg cgtgcgcagc ctcaccacgt cgccggcgcg cgccttgagc
    13381 agcgcgcgcg cgatcggcga gagccacgac acgcgcccgc gcgccgggtc cacctcgtct
    13441 gcgccgacga tggtgatgct gcgttgctcg cctgtacgca aagcgacacg caccgtggcg
    13501 ccgaagaaga cctgctcggt gtcgcggccg gcgttgtcga cgacgctcgc gttctccagc
    13561 cgcttgatga ggaagcgcac ccggcggtcg atctcgcgca ggcgcttctt tccgtagatg
    13621 tagtcggcgt tctccgagcg gtcgccgttg gcggccgccg cggacaccgc gcgcaccacc
    13681 tccgggcgct cgcgctccac caggcgccgg cgctcggcgt cgaggcgcgc gaagcccgcc
    13741 ggcgtgatgt agttcttcgc cggcaccggc gcatcggcgc gctcgtcttc ctcgccggcg
    13801 ccgtcggatt cccggacgaa cgctttcgac atgcaggcgc gattctaccc gcaccttgct
    13861 tgcgcgcgcg gtgccggtcg ccgagaatcc gcccgtggca tttcaacgaa aggtcgacag
    13921 ggacgatctg ctcacgcgcc tgcgcgtgcg cgaggtcgcg ctgttcagcc tgatcgcgct
    13981 caccgccatc ctcgccaacc tgccggagag ctacgcgcgc cagctcggca tccagcacga
    14041 ctacctgtac gcggcgctgg tcggcgcggt ggtcatcggc ctgttcctgt acctgaagtt
    14101 cttcttcttc ttcgccgtgg tcctgctgat cctcggggcc aacctgcccg agcagatcgc
    14161 gcaagatttc ggcatctcga agatccccct gatcctggcg ctggtagcga tggtcggggt
    14221 gtcgctcatc aattacgtgg tgaaggtgct gcccacgggc ctcgagccca agccgcgcga
    14281 gaagagcccg gaaggcgtgc gcgccctgct ctacgccatc gacaagaaca acgtggtcta
    14341 cggccagcgc gtgctggcga tgaacttcga cccgaacctg caccatgaga acggctacac
    14401 cccgctcgcc tacgcggcgc tcaagggcaa cccgcagatg atcgagatgc tgctggggaa
    14461 cggcgccgat ccgggcctca cgacgcgcga gggcgagacc ccggtcgagc tcgccctgcg
    14521 cgcggggcac accggcctcg ccgacctgct caagcaggcg cgccaggcgc gcgaaaccgc
    14581 acttgcggcc gggtcgcctc cagggtgaca atactgtata aataagcagt ttagtgcagg
    14641 ctgagccgcc atgaacgaaa cccccgtcgt ccccgccatc gccccgcagg cgatctgcgt
    14701 cgacgccctg ctcgagaagt acgccaaggg cggcgaagcc accgtcggcg aggtgcgtgc
    14761 gcgggtcgcg cgcgcgctcg cgcaggccga gcccgaggcg cgccgatccc actgggagcg
    14821 gcgcttcctg caggcgctcg aggacggctt catccccgcc gggcgcatca actccgcggc
    14881 cggggtgcag ctgcaggcga cgctcatcaa ctgcttcgtg cagccggtgg gcgattcgat
    14941 ctcggaggtc gtcgacggca agccgggcat ctataccgcg ctgcaggagg ccgccgagac
    15001 catgcgccgc ggcggcggcg tcggctacga cttttcctcg atccgcccga agggcgccga
    15061 ggtgaagggc acgcgctcgc gcgcgagcgg cccggtgtcc tacatgcgcg tgttcgaccg
    15121 ctcgtgtgag acggtggaat cggcgggcgc acgccgcggc gcgcagatgg gcgtgctgcg
    15181 ttgcgaccac ccggatatcg aggacttcat ccacgccaag gaccagggcg acctcgccaa
    15241 tttcaatgtc tcggtcggcg tcaccgacgc cttcatgcag gcggtggagc aggacgccga
    15301 ggtcgagctg gtgcacaagg cgaagccctg gcccgggccg gacgtcgcgc agcgcgccga
    15361 cgggctgtgg gtgtaccgca aggtgcgcgc gcgcgcgctg tgggatcaga tcatgcgctc
    15421 gacctacgat cacgccgagc cggggatcct gttcctcgac cgcatgaacc gcgacaacaa
    15481 cctcaactac tgcgaggcga tcgaggcgac caacccgtgc gccgagcagc cgctgccgcc
    15541 ctacggctgc tgctgcctcg gctcgatcga cctgacgcgc ttcgtgcgca atgccttcgc
    15601 cgccgacgct gcgttcgact acgaggcatt cggcgccgtg gtcgacgtcg ccgtgcgcat
    15661 gctcgacaac gtgctcgagg tgaccgcctg gccactcgag gcgcaacgac gcgaggcgat
    15721 ggccaagcgc cgcgtcggcc tgggcttcac tggcctgggc gacgcgctca tcatgctgcg
    15781 ccagcactac gacagcgacg cggcgcgcgc cgccgccgcg cgcatttccg ccgcgatgcg
    15841 cgaccgcgcc tacctcgcct cggcggcgct ggccgaagag cgcggcgcgt ttccgctgtt
    15901 caacgccgat ctgtacctgt cgggcaccag cttcgccgcg cgcctgcccg aggaggtgaa
    15961 ggcgccgatc cgcgcacacg gcatccgcaa ttcgcacctg ctgtcgattg cgcccaccgg
    16021 caccatcagc ctggcgttcg ccgacaacgc ctccaacggc atcgaaccgc ccttctcctg
    16081 gacctacacg cgcaagaagc gcatgcccga cggcacgctg caggagtttc cggtcgagga
    16141 ccacgcctgg cggcgctaca aggccgccgg cggcgacatg gaaaacctac cgccccactt
    16201 cgtcaccgcg ctcgagctct cggcggcggc gcacgagcgc atggtggccg cggtcgctcc
    16261 gttcatcgat accagcatct cgaagactgt caacgtgccg gaggactacc cctacggcga
    16321 gttcaaggac ctgtacctcg cggcgtggaa gtccggcctc aagggcctcg ccacctaccg
    16381 gccgaacaag gttctcggct cggtgctctc ggtggacacg ccgcaggact tcgtcgagga
    16441 cgacgtcaac cgccgtatcc gcatcaagac gctgcccgag ccggtgctcg cgtccctgcg
    16501 ctggccggga cggccggagt ttgcggccgg gaatctgtcc tggacctaca tgatcgagca
    16561 tccgcagcac catttcgcgg tgttcgtggg cgaggcggag aacggcggcc cgcacgcctt
    16621 cgaggtctgg gtgaacggca gcgagcagcc gcgcggcctg ggcgcggtgg caaagtcgct
    16681 ttccatggac atgcgcgccg aggacccggg gtggctgcgc ctcaagctcg acacgctcgc
    16741 gcgcaccgca ggcgacgacg ccttcgacat gtcgttcccg ccgcgcggcg agaagcgccg
    16801 catgccctcg gtggtctcgg cgctggcgca attggtgcgc tggcgcgtgg agcagctcgg
    16861 cggcctcgac ggcgagaagc aggccgacgc cttcgccagc gtgacgccgg tggtcgactc
    16921 gatgttcagc ctgaaggagc ccaagaccgg cgccgacggc accatgtcct ggaccgtgga
    16981 cgtgcacaac ccgcgcaccg gcgacgactt cgtgctcggc cttaaggaaa tcgcgctgcc
    17041 cgacggcacc acgcgcccct actcgatgtg gctctcgggc gagtacccgc gcgcgctcga
    17101 cggcctgtgc aaactgctct cgctcgacat gcgcgtggtc gacccggcgt ggatcggcat
    17161 gaaattgaag aagctgctcg atttcccgga accgctgggc gactttctcg ccttcgtgcc
    17221 cggttcgcga aagcagcaga gctacccttc gacggtgtcg tacctggcgc gcctcatcct
    17281 gcaccgctac gccatgcttg gcctcctcga cgaggacggc gcaccgct
//
LOCUS       DBNHKE010000243        11707 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1105257, whole genome shotgun sequence.
ACCESSION   DBNHKE010000243 DBNHKE010000000
VERSION     DBNHKE010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11707
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1105257"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1482
                     /gene="murC"
                     /locus_tag="AC16O9_16580"
     CDS             106..1482
                     /gene="murC"
                     /locus_tag="AC16O9_16580"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6518271.1"
                     /translation="MKHKVRHIHFIGVGGSGMSGIAEVLLNLGYQVSGSDLAESPATR
                     RLAELGARVATAHAAHNIDGADAVVVSSAVRADNPEVQAARARRVPVVPRALMLAELM
                     RLKQGVAIAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLNAAGSNARLGQGEYIVVE
                     ADESDASFLHLQPVLAVVTNIDADHMETYQHDFARLKQAFVQFLQNLPFYGSAVLCAD
                     DAHVREILPFVSKPVVTYGFAAEAMVRAERVEHAAGAMRFTTQREGKPALSVHLNLPG
                     AHNVQNALAAIAVGTELGVADAAILKALAEFRGVGRRFQRCGEIKVEGGGRFTLVDDY
                     GHHPAEMAATLAAARGAFPGRRMVLAFQPHRYTRTRDLFEDFVEVLSTADALLLAEVY
                     AAGEPPIVAADGRSLARAVRVAGKVEPVFVEDVAQLAAAIRRVARDGDVVVTMGAGSI
                     ANVPAQLT"
     gene            1479..2402
                     /gene="murB"
                     /locus_tag="AC16O9_16585"
     CDS             1479..2402
                     /gene="murB"
                     /locus_tag="AC16O9_16585"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR6518272.1"
                     /translation="MNMADALRLQGLRGVLKAREPMSRHVSWRAGGAAARFYAPADLE
                     DLQSFLAQLPPREPLLFVGLGSNLLVRDGGFNGTVVLTHAAGLHPQTLGGRLYADAGV
                     AAPKVARFAARHDLAHAEFLAGIPGTVGGALAMNAGCYGGETWDLVERAVTIDRSGAL
                     RTRARAEFDVGYRHCALRDGRRLGEDEWFAGAWFALRPGDGAVARGRIRELLVRRIAS
                     QPLQLPNAGSVFRNPPGDHAARLIESCGLKGLARGGARVSEKHANFIVNPERRASAAD
                     IEGLIEEVRRRVAERTSVELMPEVRIVGEAA"
     gene            2399..3310
                     /locus_tag="AC16O9_16590"
     CDS             2399..3310
                     /locus_tag="AC16O9_16590"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR6518273.1"
                     /translation="MSPGRVAVLMGGRSAEREVSLMSGTGVLEALRAKSVDAHGFDPA
                     ERDLGELKREGFARCFIALHGRGGEDGTVQGALEVMGIPYTGSGVLGSAVALDKWRAK
                     MIWLAGGLPTPRYRILQANDDWDAVAADLGLPLIVKPANEGSTLGLTKVTAVAQLPAA
                     YELAAKKYRDVALAECFVDGPEYTAGILGETVLPLIRIEAPQGNYDYQHKYFSDDTKY
                     LCPCGLAPEQEAKLKALSLEAFRLLGCSGWGRVDLMLDEAGEPWLLEVNTSPGMTGHS
                     LVPIAARAVGMSYEDLCLKILEGAHVG"
     gene            3300..4019
                     /locus_tag="AC16O9_16595"
     CDS             3300..4019
                     /locus_tag="AC16O9_16595"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6518274.1"
                     /translation="MWDNPRLLNAAAGALTALALMVFAVAAMQLLLRSELFPLRELTV
                     RGSLAHTSHADIERAAHGRVNGNFFAVDLAQVRAALEALPWVRRVEVRRVWPDRLEAR
                     LEEHVALARWAAGGLVNLQGERFAGASDAILPQFAGPAGSEADVTRRYRRFTEILAPL
                     ERVPERVALSPRYAWRLRLDDGLAIELGRDAPQDPVEARLARLVAAWPQTLARMPMKH
                     EHVDLRYPNGFALRVAEAKGG"
     gene            4023..5252
                     /gene="ftsA"
                     /locus_tag="AC16O9_16600"
     CDS             4023..5252
                     /gene="ftsA"
                     /locus_tag="AC16O9_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6518275.1"
                     /translation="MAKETKNLVVALDIGTSKVAALVAELRADGSLEILGMGGHESKG
                     MKKGVVVNIEATVNAVQRALEEAELMADCKIASAFTGIAGSHIKSFNSTGMVAIKDRE
                     VGALDVERAIETAKAVNIPTDQQILHVLRQEFIIDGQEDVREPIGMSGVRLEVKVHIV
                     TGAVSAAQNIVKCVRRCGLEVNDLILQPLASSRAVLSEDETDLGVCLVDVGGGTSDIA
                     VFTHGAIRHTAVIPIAGDQITNDIAMALRTPTAEAEVIKVRHGVALRQLADPNEMIEV
                     PGIGDRGPRVMSRQTLAEVIEPRVEELFSLVQQVLRESGFEELLSSGVVLTGGSSVMR
                     GMVELGEEIFHMPVRVGVPRYAGGLADVVRSPRYATAVGLLMEGVQQVRQGQLSRHDG
                     SMRAVLSRMREWFQRNF"
     gene            5283..6449
                     /gene="ftsZ"
                     /locus_tag="AC16O9_16605"
     CDS             5283..6449
                     /gene="ftsZ"
                     /locus_tag="AC16O9_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6518276.1"
                     /translation="MFEIVDSQAAGPVIKVIGVGGAGGNAVNHMIEQGVEGVEFIAVN
                     TDAQVLSRNRSRNQIQLGSSGLGAGAKPEQARAVAMAERDRIEEAVSGAHMVFITAGM
                     GGGTGTGAGPVIAEVARSLGILTVAVVTKPFSFEGSKRTKIAEQGLAELAPHVDSLIV
                     ILNDKLEEVLGEDVTQEEAFRAADNVLNDAVAGIAEIINQPGLVNVDFQDVRTVMSEQ
                     GMAMMGSAVAAGVDRARIAAEQAVACPLLEGVNLAGARGVLVNISASRTSLKLRETKE
                     VMNTIRAFAAEDATIIYGGVYDDGLTDNLRVTVVATGLGAPLMARQGKPQLVVSQKTG
                     TDDQPMTGQGAVDYQQLDSVPAVIRRNRASTVEALQQSGVDKYDIPAFLRKQAD"
     gene            6552..7466
                     /gene="lpxC"
                     /locus_tag="AC16O9_16610"
     CDS             6552..7466
                     /gene="lpxC"
                     /locus_tag="AC16O9_16610"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR6518277.1"
                     /translation="MLKQRTLKSLIRAKGVGLHTGQKVNMTLRPAQPDTGIVFRRIDL
                     ASPVDIPARADRVGETRLSSCLVHQEVKVYTVEHLMSALAGLGVDNAYVDLDGPEVPI
                     MDGSAAPFALLLQQAGLAEQMAPKRFLRVTRAIEVREGDKWARLDPYEGLRLAFSIVF
                     NHPVIDKTSQSVSVDFAETSYLKEIARARTFGFMHDVERLREDGLALGGGLDNALVLD
                     EYRVLNAEGLRFADEFIRHKVLDALGDLYLLGRPLLGAFSAHKSGHALNNRLLRALVD
                     DTGAWEMASFEHAEQAPAGVAGLAWLPV"
     gene            7467..7652
                     /locus_tag="AC16O9_16615"
     CDS             7467..7652
                     /locus_tag="AC16O9_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518278.1"
                     /translation="MLLARVLGLLVAAGLGGCVLMYLATGERRYLRYAWLIFKYAVFL
                     AAFVLVLIFAERLAGEF"
     gene            complement(7579..8040)
                     /locus_tag="AC16O9_16620"
     CDS             complement(7579..8040)
                     /locus_tag="AC16O9_16620"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR6518279.1"
                     /translation="MERVVAKARELRALTRACLDFLPPGLGSQVSAANLRDGELSLVA
                     ANPAAAAKLKLVAESLRKFLLLQGTKVSSVSVRVQPTRSHREAHPPAPRVALSEGGKA
                     ELSELYSRLSPDSPLHRALGVLLKRQNVKTRPQGAPQRSARGRKQRGTPRT"
     gene            8113..9063
                     /locus_tag="AC16O9_16625"
     CDS             8113..9063
                     /locus_tag="AC16O9_16625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6518280.1"
                     /translation="MQIIVIPSRLAKARTLNVSTRHLVASLFAALALLVVMTTALYWV
                     TFRFATDIKVPLVQELVLSAQQGEAERARQFVQQNLNAMAVKLGEMQAQLARLDALGD
                     RLSSLAGIRPQEFRFSEAPGLGGAQPTALPPQNLSLAEFGEKLYVVSRQMENRTDMLG
                     VLEAQLFEQAVKKKLIPTMMPVNAPFNASGFGMRIDPFTGMQAMHEGIDFLADLGSSI
                     VAAAGGVVIFAGFHPQYGNVVDIDHGNDLVTRYAHASKLHVREGDVVQRGRRIADVGT
                     TGRSTGPHLHFEVRFKGVAQNPARFVLASQGAQHSARAGR"
     gene            9155..>11707
                     /gene="secA"
                     /locus_tag="AC16O9_16630"
     CDS             9155..>11707
                     /gene="secA"
                     /locus_tag="AC16O9_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6518281.1"
                     /translation="MANVLTKIFGSRNERLLKQYSRTVARINALETGIAALSDEALRA
                     KTVEFRTRLSERLSAVAEAERDAAEAEALEALLPEAFAVVREASKRTLRMRHFDVQLV
                     GGMVLHDGKIAEMKTGEGKTLVATLPAYLNALAGYGVHIVTVNDYLARRDAEWMGKIY
                     RFLGLSVGVNIPQMEPDEKRAAYAADITYGTNNEFGFDYLRDNMAMRVEERFQRRLRY
                     GIVDEVDSILIDEARTPLIISGQAEDRTEIYARMNEVAPLLTRQKDEKGEGDFWVDEK
                     NHQISLSEAGHAKAEEVLARVGLLPQGASLYEPAYINLVHHLHASLRAHNLFHRDQHY
                     VVQDGDVIIVDEFTGRLMHGRRWSDGLHQAVEAKEKAAIQNENQTLASITFQNYFRMY
                     RKLAGMTGTADTEAYEFQEIYGLETVVIPTHKKMIREDRLDLVYRTAPEKHKAIIEDI
                     KDCYTRGQPVLVGTTSIESSELLSGLLTKEKLPHQVLNAKQHAREAEIVAQAGRPKMV
                     TIATNMAGRGTDIVLGGNVDKQVELVEADDKVAPEDKKSRAERLRGEWQQLHDHVIRE
                     GGLHIIGTERHESRRVDNQLRGRSGRQGDPGSSRFYLSLEDPLLRIFAGERINKIMVM
                     LRMPEGEAIEHGMVTRSIESAQRKVEARNFDIRKQLLEYDDVANDQRKTIYALRNELL
                     ESSDVSARIADLRAGVVADLFRVHLPEESVEEQWDVKGLEGGLKAELNLELPVARWLE
                     QEPDLGDEALLARITEKAHEAYHAKIPSGAEDSFRQYERYVMLQILDGHWREHLAALD
                     HLRQGIHLRGYAQKNPKQEYKREAFELFSLMLEAIRNEVTRILMTVRIRSAEE"
BASE COUNT         1914 a         3886 c         4150 g         1757 t
ORIGIN      
        1 ggaactggac cggccgcggc tcctggacat ggcgcgcaag gcgcgcgcgc tgggcaagcc
       61 cgacgccgcg catcgggtgg ccgcgcgctg catggagatt gccggatgaa gcacaaggtc
      121 cggcacatcc acttcatcgg cgtcggcggc tccggcatgt cgggtatcgc cgaggtgctg
      181 ttgaacctgg gctaccaggt gagcggctcg gacctcgccg agagcccggc gacgcggcgc
      241 ctcgccgaac tcggcgcgcg cgtggcgacg gcgcacgcgg cgcacaacat cgacggcgcc
      301 gacgcggtgg tggtgtcgag cgcggtgcgc gccgacaacc ccgaagtgca ggcggcgcgc
      361 gcgcggcgcg tgccggtcgt gccgcgcgcg ctgatgctcg ccgagctgat gcgcctgaag
      421 caaggcgtcg ccatcgccgg cacgcacggc aagacgacca ccaccagcct ggtggcgagc
      481 gtgcttgccg agggtgggct cgacccgacc ttcgtcatcg gcgggcggct gaacgccgcg
      541 ggctcgaatg cgcgcctcgg ccagggcgaa tacatcgtcg tcgaggcgga cgaatccgac
      601 gcttcgttcc tgcacctgca gccggtgctg gcggtagtca ccaacatcga cgccgaccac
      661 atggaaacct accagcacga tttcgcgcgc ctcaagcagg cgttcgtgca attcctgcag
      721 aacctgcctt tctacggcag cgcggtgctg tgcgcggacg acgcacacgt gcgcgagatc
      781 ctgccgttcg tctccaagcc cgtggtgacc tacggcttcg ccgccgaggc gatggtgcgc
      841 gccgagaggg tagagcacgc cgcgggcgcg atgcgcttca ccactcagcg cgagggtaag
      901 cctgcgctct ccgtgcacct caacctgccg ggggcgcaca acgtgcagaa cgcgctcgcc
      961 gcgatcgcgg tgggcaccga gctcggcgtg gcggacgccg cgatcctcaa ggcgctcgcc
     1021 gagttccgcg gcgtggggcg gcgcttccag cgctgcggcg agatcaaggt cgagggcggc
     1081 ggccgcttca ccctcgtcga cgactatggg caccaccccg cggaaatggc cgcgacgctc
     1141 gctgcagcgc gcggcgcctt ccccggccgc cggatggtgc tcgcctttca gccgcaccgc
     1201 tacacgcgca cccgcgacct gttcgaggac ttcgtggagg tgctgtcgac ggccgacgcg
     1261 ctgctccttg ccgaggtcta cgccgccggc gagccgccga tcgtcgccgc cgacggccgc
     1321 tcgctcgcgc gcgccgtgcg cgtcgccggc aaggtcgagc cggtcttcgt cgaggatgtc
     1381 gcgcagctcg cggccgccat ccgccgcgtg gcgcgcgacg gcgacgtggt ggtgaccatg
     1441 ggcgcgggat cgatcgccaa cgtgccggcg cagctgacat gaacatggcg gacgcgctca
     1501 gactgcaagg actgcgcggc gtgctgaagg cacgcgagcc gatgtcgcgc cacgtcagct
     1561 ggcgcgcggg cggcgcggcc gcgcgcttct acgcgccggc ggacctggag gacctgcagt
     1621 cgttccttgc gcagctgccg ccgcgcgagc cgctgctgtt cgtcggcctg ggctcgaacc
     1681 tgctggtgcg cgacggcggc ttcaacggca ccgtggtgct gacccacgcg gccgggctgc
     1741 acccgcagac gctcggcggg cggctatatg ccgacgcggg tgtcgccgcg ccaaaggtgg
     1801 cgcgcttcgc cgcgcgccac gacctggcgc acgcggagtt cctggccggc attcccggca
     1861 cggtgggcgg cgcgcttgcc atgaacgccg gctgctacgg cggcgaaacc tgggacctgg
     1921 tggagcgcgc ggtgaccatc gacaggagcg gcgcgctgcg cacccgggcg cgcgcggagt
     1981 tcgacgttgg ctatcggcat tgcgcgcttc gcgacggccg ccggctgggc gaggacgagt
     2041 ggttcgcggg cgcgtggttc gcgctgcgcc ccggggacgg cgcggtggcg cgtggacgca
     2101 tccgcgagct cctggtgcgg cgcatcgcct cgcagccact gcaattgccg aacgcgggca
     2161 gcgtgttccg caacccgccg ggcgatcatg cggcgcggct catcgagtcc tgcggcctga
     2221 agggcctcgc gcgcggcggc gcgcgcgtct cggaaaagca cgccaatttc atcgtcaacc
     2281 cggaacgccg cgcgagcgcc gccgacatcg aggggctgat cgaggaagtg cgccggcggg
     2341 tcgccgagcg gacgtcggtc gaactgatgc cggaagtgcg catcgtaggg gaggcggcat
     2401 gagccccggc agggtcgcgg tgctgatggg cggaaggtcc gccgagcgcg aggtgtcgct
     2461 gatgagcggc acgggagtgc tcgaggcgct gcgcgcgaag tcggtggacg cgcatggctt
     2521 cgaccctgcg gagcgcgacc tcggggagct gaagcgcgag ggcttcgcgc gctgcttcat
     2581 cgcgctgcac ggccgcggcg gcgaggacgg cacggtgcag ggcgcgctcg aggtgatggg
     2641 cattccgtac acgggaagcg gcgtgctcgg ctccgccgtc gccctggaca agtggcgcgc
     2701 caagatgatc tggcttgccg gcggcctgcc gacgccgcgc taccgcatcc tgcaggcgaa
     2761 cgacgactgg gacgccgtcg cggcggacct cggcctgccg ctgatcgtca agccggccaa
     2821 cgagggctcg acgctcgggc tgacgaaagt caccgccgtg gcgcaactgc ccgcagcgta
     2881 cgagcttgcc gccaagaaat accgcgacgt cgcgctggcg gagtgcttcg tcgacggccc
     2941 cgaatacacc gccggcatcc tgggcgagac ggtgctgccc ctgatccgca tcgaagcgcc
     3001 tcagggcaac tacgactacc agcacaagta tttcagcgac gacacgaagt acctgtgccc
     3061 ctgcgggctg gcgccggagc aagaggcaaa gctgaaggcg ctgtcgctcg aggctttccg
     3121 cctgctcggc tgcagcggct gggggcgcgt cgacctgatg ctcgacgagg cgggcgaacc
     3181 gtggctgctg gaagtgaaca cttcgcccgg catgaccggg cacagcctgg tgccgatcgc
     3241 ggcgcgtgcc gtgggcatgt cctacgagga cctgtgcctg aagatcctgg agggcgcgca
     3301 tgtgggataa cccgcgcctg ctcaacgccg ccgccggcgc gctcaccgcg ctcgcgctca
     3361 tggtgttcgc ggtggcagcg atgcagctgc tgctgcgctc cgaactgttt ccgctgcgcg
     3421 agctgacggt gcgcggcagc ctcgcgcata cctcgcacgc cgacatcgag cgggcggcgc
     3481 acggccgcgt caatggcaac ttcttcgccg tggacctggc gcaggtgcgc gccgcgctcg
     3541 aggccctgcc ctgggtgcgt cgcgtcgaag tgcgccgcgt gtggcccgat cggctcgagg
     3601 cgaggctgga ggagcacgtc gcgctggcgc gctgggccgc gggcggcttg gtcaacctgc
     3661 agggcgagcg cttcgccggg gccagcgacg cgatcctgcc gcagttcgcg gggccagcgg
     3721 gcagcgaagc cgacgtgacg cgccgctacc gccgcttcac cgagattctg gcgccgctcg
     3781 agcgcgtgcc ggaacgcgtg gcgctctcgc cccgctacgc ctggcgcctg cgcctggacg
     3841 acggcctcgc catcgaactc ggccgcgacg cgccgcagga cccggtcgag gcgcgcctgg
     3901 cgcgcctggt ggcggcctgg ccgcaaaccc tcgcgcgcat gccgatgaag cacgagcacg
     3961 tcgacctgcg ctaccccaac ggtttcgcgc tgcgcgtggc cgaggcgaag ggcggatagg
     4021 ccatggccaa ggaaaccaaa aacctcgtcg tggcgctcga tatcggcacg tccaaggtgg
     4081 ccgcgctggt ggcagagctg cgcgccgacg gctcgctcga gatcctcggc atgggcgggc
     4141 acgagtccaa gggcatgaag aagggcgtgg tggtgaacat cgaggccacg gtgaacgcgg
     4201 tgcagcgcgc gctggaggag gccgagctga tggccgactg caagatcgcc tcggccttca
     4261 ccggcatcgc cggcagccat atcaagagct tcaactccac cggcatggtg gcgatcaagg
     4321 accgcgaggt gggtgcgctc gacgtcgagc gcgcgatcga gacggccaag gcggtgaaca
     4381 ttcccaccga ccagcagatc ctgcacgtgc tgcgccagga attcatcatc gacggccagg
     4441 aggacgtgcg tgagccgatc ggcatgtccg gcgtgcggct ggaagtgaag gtgcacatcg
     4501 tcactggcgc ggtgtcggcg gcgcagaaca tcgtcaagtg cgtgcgccgc tgcggacttg
     4561 aagtgaacga cctcatcctg cagccgctcg cctcgtcgcg ggcggtgctc tcggaggacg
     4621 agaccgacct cggcgtgtgc ctggtggacg tcggcggcgg caccagcgac atcgccgtat
     4681 tcacgcacgg cgccatccgc cacacggcgg tgatcccgat cgccggcgac cagatcacca
     4741 acgacatcgc catggcgctg cgcacgccga ccgcggaggc cgaggtgatc aaggtgcgcc
     4801 acggcgtggc cctgcggcag ctcgccgacc cgaacgagat gatcgaagtg cccggcatcg
     4861 gcgaccgggg cccccgcgtc atgtcgcgcc agacgctcgc cgaggtgatc gagccgcgcg
     4921 tcgaggagct gttttcgctg gtgcagcagg tgctgcgcga atccggtttc gaggagctgc
     4981 tctcctccgg cgtggtgctg accggcggca gctccgtcat gcgcggcatg gtcgagctgg
     5041 gcgaggagat cttccacatg ccggtgcgcg tgggcgtgcc gcgctacgcg ggcgggctcg
     5101 ccgacgtggt gcgcagcccg cgctacgcca cggcggtggg actgctgatg gagggcgtgc
     5161 agcaggtgcg gcagggacag ctgtcgcgcc acgacggatc gatgcgtgcc gtgctttcgc
     5221 gcatgcgcga gtggtttcag aggaatttct gacgttggtt caactggcaa ggaggaaaac
     5281 gcatgtttga gatcgtggac agtcaagcgg cagggccggt catcaaggtg atcggggtcg
     5341 gtggcgccgg cggcaacgcc gtcaatcaca tgatcgagca gggcgtcgaa ggcgtggagt
     5401 tcatcgccgt caacaccgac gcgcaggtgc tttcgcgcaa ccgctcgcgc aaccagatcc
     5461 agctcggctc cagcgggctc ggggcgggcg ccaagcccga gcaggcgcgc gcggtggcga
     5521 tggccgagcg cgaccgcatc gaggaggcgg tctcgggcgc gcacatggtg ttcatcaccg
     5581 ccggcatggg cgggggcacc ggcaccggcg ccggcccggt gatcgcggag gtggcgcgtt
     5641 cgctcggcat cctcaccgtg gcggtggtga ccaagccgtt ctcgttcgag ggctcgaagc
     5701 gcacgaagat cgccgagcag ggactcgcgg agcttgcgcc gcacgtggat tcgctcatcg
     5761 tgatcctcaa cgacaagctc gaggaggtgc tgggcgagga cgtgacgcag gaggaagcgt
     5821 tccgcgccgc cgacaacgtg ctgaacgacg cggtcgcagg tatcgccgag atcatcaatc
     5881 agccgggact ggtgaatgtc gacttccagg acgtgcgcac cgtcatgtcc gagcagggca
     5941 tggcgatgat gggctcggcc gtcgctgccg gcgtggaccg cgcgcgcatc gccgccgagc
     6001 aggcggtcgc ctgcccgctg ctcgaaggcg tgaacctcgc cggggcgcgc ggcgtgctgg
     6061 tcaacatcag cgcctcgcgc acgagcctca agctgcgcga aaccaaggag gtgatgaaca
     6121 ccatccgcgc attcgcggcg gaggatgcca ccatcatcta cggcggcgtg tacgacgacg
     6181 gccttaccga caacctgcgc gtgacggtcg tggcgaccgg gctgggcgca ccgctgatgg
     6241 cgcgccaagg caagccccag ctcgtggtat cgcagaagac cggcaccgac gaccagccga
     6301 tgaccggcca gggcgccgtc gactaccagc aactcgactc ggtgcccgcg gtgatccggc
     6361 gcaaccgggc ctcgacggtc gaggcgctgc agcaaagcgg ggtcgacaag tacgacattc
     6421 cggcctttct gcgcaagcag gcggactagg atccgcgcgg gctcgggcgg ggcgcctctt
     6481 ttctcctcct agccggaggc gctccgccgt gctcgtctgc tagaatccca agttcgctca
     6541 agaagttagg catgctcaag caacgcaccc tgaagtccct gattcgcgcc aagggcgtcg
     6601 gcctgcacac cggacagaag gtcaacatga cgctgcggcc ggcgcagccc gacaccggca
     6661 ttgtctttcg ccgcatcgat cttgcttcgc ccgtggacat cccggcgcgc gccgatcgcg
     6721 tgggcgaaac gcggctgtcg tcgtgcctcg tgcaccagga ggtgaaggtg tacaccgtcg
     6781 agcacctgat gtcggcgctc gccggcctgg gcgtggacaa cgcttacgtg gacctggatg
     6841 gtccggaagt gccgatcatg gacggcagcg cagcgccgtt cgcgctcctg ctgcaacagg
     6901 cgggcctcgc cgagcagatg gcccccaagc gcttcctgcg cgtgacgcgg gccatcgagg
     6961 tgcgcgaggg cgacaaatgg gcgcgcctcg atccctacga gggactgcgg ctcgcgttct
     7021 ccattgtttt caaccatccg gtgatcgaca agaccagcca gtcggtgagc gtcgacttcg
     7081 ccgagacctc gtacctcaag gaaatcgcgc gcgcgcgtac cttcggcttc atgcacgacg
     7141 tcgagcgcct gcgcgaggat ggcctcgcgc tcggcggcgg cctggacaac gcgctggtgc
     7201 tggacgaata ccgggtgctc aatgccgagg gcctgcgctt tgccgacgag ttcatccgcc
     7261 acaaggtgct cgatgcgctc ggcgacctgt acctcctcgg gcggccgttg ctcggcgcct
     7321 tctcggcgca taagtccggg catgcgctga acaaccggct gctgcgcgcg ctggtcgacg
     7381 acaccggcgc ctgggagatg gcgagcttcg agcatgccga gcaggcgccg gccggcgtgg
     7441 ccggcctagc ttggttgccg gtctaggtgc tgctggcgcg cgtcctcggg ctgctggtgg
     7501 cggccggact tggcggctgc gtgctgatgt acctcgcgac cggcgagcgc cggtacctgc
     7561 gctacgcgtg gctcatcttc aagtacgcgg tgttcctcgc tgctttcgtc ctcgtgctga
     7621 tctttgcgga gcgccttgcg ggcgagtttt gacgttttgg cgttttagca acacgccgag
     7681 ggcacggtgg agcggggaat ccggactcag gcggctgtaa agctctgata attcagcttt
     7741 tccgccctcc gaaagggcga cccggggtgc cggcggatga gcctcccggt gtgaccgagt
     7801 tggttgcacc cttaccgaaa ctgaactaac cttcgttccc tgtagtaaca agaacttacg
     7861 caagctctcg gcgaccagtt tgagcttcgc ggcggctgcc ggattggcgg caaccagcga
     7921 aagctcgccg tcgcgcagat tggcggcgct tacttggctc cccagtccgg gcggtaggaa
     7981 atcgaggcaa gcgcgggtga gcgcgcgcag ttcgcgcgcc ttggcgacga cgcgttccat
     8041 gcccgcgtcg gtgatcaggt agcgatcggc tttggctggg tgcaagggga gttcggaaac
     8101 agggagcgcc gagtgcagat tatcgtaatc ccgagtcgtc tggccaaagc gcggacgctg
     8161 aacgtgtcga cgcggcactt ggtcgcgtcg ctgttcgccg ccctggcgct gctggtggtg
     8221 atgaccacgg cgctgtactg ggtcaccttc cgctttgcca ccgacatcaa ggtacccttg
     8281 gtgcaggagc tcgtgctctc cgcccagcag ggcgaagccg agcgcgcgcg ccagttcgtg
     8341 cagcagaacc tcaacgccat ggccgtgaag ctcggcgaga tgcaggcgca gctcgcacgc
     8401 cttgacgcgc tcggcgaccg cctgtcgtcg ctggccggca tccggccgca ggaattccgc
     8461 ttcagcgagg ccccgggcct gggcggcgcg cagccgaccg cgctgccgcc gcagaacctg
     8521 tcgcttgccg agttcggcga gaagctgtac gtggtgtcgc gccagatgga aaaccggacc
     8581 gacatgctcg gcgtgctgga agcgcagctg ttcgagcagg cggtgaagaa gaagctgatt
     8641 ccgaccatga tgccggtcaa cgcgccgttc aacgcgtccg gctttggcat gcgcatcgat
     8701 cctttcaccg gcatgcaggc gatgcacgag gggatcgact tcctcgccga cttgggctcg
     8761 tccatcgtcg ctgctgccgg cggcgtggtc atcttcgccg gatttcatcc gcagtatggc
     8821 aacgtcgtcg acatcgacca cggcaacgac ctcgtgacgc gctatgcgca cgcgtcaaag
     8881 ctgcacgtgc gcgaaggcga tgtcgtacag cgcggccgcc gcatcgccga cgtcggcacc
     8941 accggccgct ccaccggccc gcacctgcac ttcgaagtgc gcttcaaggg cgtggcgcag
     9001 aacccggcgc gcttcgtgct ggcctcgcag ggcgcgcagc acagcgcccg cgccggccgc
     9061 tagtcagacc ctcggggcgc ccgccccgcc gtgctaaaat tttgagtttc aagggtattt
     9121 ttcgcgcgtc cgccgctgcg gcggcgactt cgcaatggcc aacgtcctaa ccaagatctt
     9181 cggcagccgc aatgagcgcc tgctgaagca gtattcacgc accgtcgcgc gcatcaacgc
     9241 gctcgagacc ggcatcgcgg cgctctcgga cgaggcgctg cgcgccaaga ccgtcgagtt
     9301 ccgcacgcgg ctgtccgagc gtctcagcgc cgtagccgag gccgagcgcg acgcggcgga
     9361 agccgaagcg ctggaggcgc tgctcccgga agcctttgcc gtggtgcgtg aggcgtccaa
     9421 gcgcaccctg cgcatgcgcc acttcgacgt gcagctcgtc ggcggcatgg tgctgcacga
     9481 cggcaagatc gccgagatga agaccgggga gggcaagacc ctggtggcca cgctaccggc
     9541 gtacctcaac gcgcttgcgg gctacggcgt gcacatcgtg acggtcaacg actacctggc
     9601 gcggcgcgac gcggaatgga tgggcaagat ctaccgcttc cttgggctga gcgtgggcgt
     9661 caacatcccg cagatggagc cggacgagaa gcgtgccgcc tacgcggccg acatcaccta
     9721 cggcaccaac aacgagttcg gcttcgatta cctgcgcgac aacatggcga tgcgagtcga
     9781 ggagcgcttc cagcgccgtc tgcgctatgg catcgtcgac gaggtggact cgatcctgat
     9841 cgacgaggcg cgcacgccgc tgatcatctc cggccaggcc gaggaccgca ccgagattta
     9901 cgcgcgcatg aacgaggtgg cgccgctgct tacgcgccag aaggacgaga agggcgaggg
     9961 agatttctgg gtcgacgaga agaaccacca gatttcgctc tcggaggccg gccacgccaa
    10021 ggccgaagag gtcctcgcgc gcgtcggcct gctgccgcag ggcgcgagcc tgtacgagcc
    10081 ggcctacatc aatctggtgc accacctgca cgcgtcgctg cgtgcgcaca accttttcca
    10141 ccgtgaccag cactacgtgg tgcaggacgg cgacgtgatc atcgtcgacg agttcaccgg
    10201 gcgcctgatg cacgggcggc gctggtcgga cggcctgcac caggcggtcg aggcgaagga
    10261 aaaggcggcg atccagaacg aaaaccagac gctggcgtcg atcaccttcc agaactactt
    10321 ccgcatgtac cgcaagctcg cgggcatgac cggcaccgcc gacaccgagg cctacgagtt
    10381 ccaggagatc tacggcctcg agacggtggt catcccgacg cacaagaaga tgatccgcga
    10441 ggaccggctc gacctcgtgt accgcactgc gccggagaag cacaaagcga tcatcgagga
    10501 catcaaggac tgctacacgc gcgggcagcc ggtgctggtg ggcaccacgt cgatcgagtc
    10561 ttccgagctg ctgtcgggcc tgctgacgaa agagaagctg ccgcaccagg tgctgaacgc
    10621 caagcagcac gcacgcgagg ccgagatcgt ggcgcaggcc ggacggccga agatggtcac
    10681 catcgccacc aacatggcgg ggcgcggcac cgacatcgtg ctcggcggca acgtcgacaa
    10741 gcaggtagag ctggtggagg cggacgacaa ggtcgcgccg gaggacaaga agtcgcgcgc
    10801 cgagcgcctg cgcggcgagt ggcagcaact gcatgaccac gtgattcgcg agggcggcct
    10861 gcacatcatc ggcaccgagc gccacgagtc gcgccgggtg gacaatcagc tgcgcggccg
    10921 ctcgggccgc cagggcgacc cgggcagctc gcgcttttac ctgtcgctcg aggacccgct
    10981 gctgcgaatc ttcgccggcg agcgcatcaa caagatcatg gtgatgctgc gcatgccgga
    11041 gggcgaagcc atcgagcacg gcatggtgac gcgctcgatc gagagcgcgc agcgcaaggt
    11101 cgaggcgcgc aacttcgaca tccgcaagca gctgctggaa tacgacgacg tcgccaacga
    11161 ccagcgaaag accatctacg cgctgcgaaa cgagctgctc gaatcgagcg acgtatcggc
    11221 gcgcatcgcc gacctgcgcg ccggcgtggt cgcggacctg ttccgggttc acttgcccga
    11281 ggagagcgtc gaggagcagt gggacgtgaa agggcttgag ggcgggctca aggccgagct
    11341 caacctggag ctgccggtcg cacgctggct cgagcaggag ccggatctcg gcgatgaggc
    11401 gctgctcgcg cgcatcaccg agaaggcgca cgaggcgtat cacgccaaga tcccctcggg
    11461 cgccgaagac tcgttccgcc agtacgagcg ctatgtgatg ctgcagatcc tggatgggca
    11521 ctggcgcgag catctcgccg cgctcgatca cctgcgccag gggatccacc tgcgcggtta
    11581 cgcgcagaag aacccgaaac aggaatacaa gcgcgaagcg tttgaattgt tctcgctgat
    11641 gctcgaggca attcgtaatg aggtcacccg tatcctcatg acggtccgga ttcgcagcgc
    11701 cgaagaa
//
LOCUS       DBNHKE010000244         6637 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_737025, whole genome shotgun sequence.
ACCESSION   DBNHKE010000244 DBNHKE010000000
VERSION     DBNHKE010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_737025"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="AC16O9_16635"
     CDS             complement(<1..475)
                     /locus_tag="AC16O9_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6518282.1"
                     /translation="MRAGWLAALMFSGCALAQPAKVSTERLALCGACHGEQGVSRIPG
                     TPSLAAQPSLFIENQLVLIREGVREVAAPMAEAVQGMRDAEIRALAKHYAALRPPPPA
                     DAPDPALARRARAVADKAYCGSCHLPDYSGRDQIPRLAGQREEYLLESMRAYVENR"
     gene            complement(475..1668)
                     /locus_tag="AC16O9_16640"
     CDS             complement(475..1668)
                     /locus_tag="AC16O9_16640"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6518283.1"
                     /translation="MKRAVLAATLLAAMSAYAQPKPPPAWHQGKPPALADSKLAPLPG
                     RMTETPASEIPITKLKLPKGFAVEIWAAGMPGGRAMARGPNGKIYVGTRAIGRVYEVT
                     DHGTRRTSRVVVDKLTQPAGVAMRGESLYVMAINKVLRFDGIEKNPDVQPVDMTAAFN
                     LPPEQHHNWKYIAFGPDGKLYVPFGAPCNICEPPAEYAQIRRYNPDGSGMEVIARGVR
                     NSVGFDFHPQTGELWFTDHGRDWMGDNGPEDELNRVSKPGLNFGYPYCHAKGVLDADF
                     KRADGCKGVTLPVTTMGPHAAAMGVLFYTGTMFPKEYQNTMFIARKGSWNRTVKFGFD
                     VVNVRATTDGKSAKVKPFMTGFLEDPKAGKFWGRPAYLLQMPDGALLVSDEQLGAIYR
                     VSYYK"
     gene            complement(1721..3766)
                     /locus_tag="AC16O9_16645"
     CDS             complement(1721..3766)
                     /locus_tag="AC16O9_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor family protein"
                     /protein_id="HIR6518284.1"
                     /translation="MRLALAWLAVVACGAFAQPSDPVVVTATRVDRPSLDIPAAIDRL
                     YAEDVREGRLQVNLSESLGRVPGVVVQNRHNYAQDLQVSSRGFGARASFGVRGIRLIA
                     DDIPASMPDGQGQAATFDLGSAGRVEVLRGPFSALYGNASGGVILVQTEDAPPVPTLE
                     GALTAGSYGTRRAALKFGARAQGVGHAVNLSRFESDGYREHSAVRRDQLNTKLDLALG
                     RGTGLTLVGMALHQPDTQDPLGLTAAQVAQNPRQAAPAALTFNTRKSVYHDQVGVTLG
                     HEVQGVARLQVSAWGGGRDVVQYLSIPLAAQSAPTASGGVVDLERVFGGIALRGQRDM
                     AAGGRPLRLAAGLEYERMDERRRGFINNNGVAGALKRDEDNAVHASDAYVQAEWRFAE
                     RWSAHAGLRSSRVEFKTADNFIAAGNPDDSGSVTFEANTPVAGILYRLDALTSLYANY
                     GRGFETPTFVELAYRSVGTGLNFDLVPSRSRHVEAGFKAVRPGLGRLNLAVFDIATRD
                     EIVVDQSSGGRTTFRNAGRTERRGIELGAESLLAGPFEARAAYTWLDAAFASDFGTAA
                     AGRQLPGVPEVQFYAEGAWRHAPLGLRAALELLHRSRVPVNDANSEFAGSFTVANAVL
                     GFEQRAGRWRLAEFLRVDNLADRAYVGSVIVNDGNGRFYEPAPQRNIVLGIQANLRF"
     gene            3788..4123
                     /locus_tag="AC16O9_16650"
     CDS             3788..4123
                     /locus_tag="AC16O9_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518285.1"
                     /translation="MPPRGSRLACTLKTIDGCQGSFDVTPGEQPNSIAAVGEINWNKA
                     PGKPVQEGAFTVIGDLGMTGQVILVNQYQWRALTEAKLEKFFYAAMLWGKSPFKVIED
                     AQLILKRGK"
     gene            complement(4136..4792)
                     /gene="msrA"
                     /locus_tag="AC16O9_16655"
     CDS             complement(4136..4792)
                     /gene="msrA"
                     /locus_tag="AC16O9_16655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6518286.1"
                     /translation="MFAFMKKMTQLLPKDSTLPGRKEKMAVPETHAVNGNRMVPPFPQ
                     GLQLAQFGLGCFWGAERIFWQTPGVYSTAVGYAGGETPNPTYEEVCSGFTNHTEAVLV
                     VFDPKRVSYEALLKVFWEGHDPTQGMRQGNDRGSQYRSAIYTCDEAQAKAATAARDAF
                     QAALKAKGYGAITTEIKPAGEFYYAEDYHQQYLAKNPGGYCGLGGTGVSCPVGALEKA
                     "
     gene            4825..5808
                     /locus_tag="AC16O9_16660"
     CDS             4825..5808
                     /locus_tag="AC16O9_16660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6518287.1"
                     /translation="MKICIFGAGAVGGVMAGALHRAGHDVSVVARGANLTAIRERGLR
                     IRAQASGEIATHKVRAEADPAKLGPQDCVIVALKAHSLPEAARAVPALAGPDTTVVTA
                     MNGVPWWFFDRLRFGEGKLRLDSLDPDGALARAMPTERIVGCVIHFAASTPEPGLVSH
                     NMGNKLILGEPGGRNSARTQAIAGALAAAGFDCVQSDFIEKDFWVKLLGNVSFNPVSA
                     LTLATADKLIADAEIKDYMVTIMREVLAIGRAVGVDADIDPEARIDMARHLGRFKTSM
                     LQDMEAGKPLEIDGLLTGTLEVAQKAGIAAPFTHSLCGLIRLRAQATGQYR"
     gene            5805..6428
                     /locus_tag="AC16O9_16665"
     CDS             5805..6428
                     /locus_tag="AC16O9_16665"
                     /inference="COORDINATES: protein motif:HMM:NF018684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR6518288.1"
                     /translation="MRALLLALLAALPLAAAASYDGPALDACRAIGERALKRDGADVK
                     ALAFDNDRHLYFERKARKLGSQPIGATLSGHGAIVRAAGPAVEIVFLCLLASEGRALW
                     FHWLPRRDAPALAQCRRSTGAGVCLEALLELAERDLLEVSALRFQESLEADARAGNDA
                     ASTAYRSSAAAWRTYRDLECARRGAAGSDAWRACRIDLTRWRYLELQ"
     gene            complement(6422..>6637)
                     /locus_tag="AC16O9_16670"
     CDS             complement(6422..>6637)
                     /locus_tag="AC16O9_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518289.1"
                     /translation="EVWAVVERGDVGLPEAAVARAGVGHLFESPHDSSAEILRRVRAA
                     GVPRRWRMASYDLGSARRGRWPRLEDH"
BASE COUNT          966 a         2365 c         2284 g         1022 t
ORIGIN      
        1 ggcggttctc gacgtacgcg cgcatcgatt cgagcaggta ctcctcgcgc tggccggcaa
       61 ggcgcgggat ctggtcgcgc ccgctgtagt ccgggaggtg gcagctcccg cagtacgcct
      121 tgtccgcgac tgcccgggcg cgccgcgcca gcgccgggtc gggtgcgtcc gcgggcggtg
      181 gcggcctgag cgctgcgtag tgcttcgcca gcgcgcggat ttccgcgtcg cgcatgccct
      241 gaaccgcctc ggccatcggc gccgcgacct cgcgtacgcc ctcgcggatc agcaccagct
      301 ggttttcgat gaacagcgac ggctgcgcgg cgagcgaggg cgtgcccggg atccgcgaca
      361 cgccttgctc gccatggcac gccccgcaga gtgcgaggcg ctcggtcgaa accttcgccg
      421 gctgcgccag cgcgcagccg gaaaacatca gggcggcgag ccagcccgcc cgcactactt
      481 gtagtagctc acacggtaga tcgcgccgag ctgctcgtcc gagaccagca gcgcgccgtc
      541 gggcatctgc agcaggtacg ccggacggcc ccagaacttg ccggccttgg gatcctcgag
      601 gaacccggtc atgaacggct tcaccttcgc gctctttccg tcggtcgtgg cgcgcacgtt
      661 gaccacgtcg aagccgaact tcacggtgcg gttccaggag cccttgcgcg cgatgaacat
      721 cgtgttctgg tactccttcg ggaacatcgt gccggtgtag aacagcaccc ccatcgccgc
      781 tgcgtgcggc cccatggtgg tcaccggcag ggtcacgcct ttgcagccat cggcgcgctt
      841 gaagtcggcg tcgagcacgc ccttggcgtg gcagtaggga tagccgaagt tcaagcccgg
      901 cttggagacc cggttcagtt catcctccgg cccgttgtcg cccatccagt cgcgaccgtg
      961 gtcggtgaac cagagctcgc ccgtctgcgg gtggaaatcg aagcccacgg agttgcgcac
     1021 gcctcgggcg atcacctcca tgcccgagcc gtccgggttg tagcgacgga tctgcgcata
     1081 ctcggcgggc ggctcgcaga tattgcacgg cgcaccgaac ggtacgtaga gcttgccgtc
     1141 cggcccgaag gcgatgtact tccagttgtg gtgctgctcg ggcggcaggt tgaacgcggc
     1201 ggtcatgtcc accggctgca cgtccggatt cttctcgatg ccgtcgaagc gcagcacctt
     1261 gttgatcgcc atcacgtaga gagattctcc gcgcatcgcg acgcccgcgg gctgcgtaag
     1321 cttgtcgacc acaacgcggc tggtgcgccg cgttccgtgg tcggtgacct cgtacacgcg
     1381 gccgatggcg cgggtaccca cgtagatctt gccgttcggc ccgcgcgcca tggcgcgacc
     1441 gcccggcatg cccgcggccc agatctcgac ggcaaagccc ttgggcagct tgagcttggt
     1501 gatcgggatc tccgaagccg gcgtctcggt catgcgcccg ggtagcggcg cgagcttgga
     1561 gtcggctagg gccggcggct tgccctggtg ccaggccggc ggcggcttgg gttgcgcgta
     1621 agcggacatc gcggcgagaa gcgtcgcggc gagcactgct ctcttcatgg cgtggttcct
     1681 ccgggtagag agggcgaata ttagtcctgc ccggaatttt ttagaaccgc aggttcgcct
     1741 ggatgccgag cacgatgttg cgctgcggcg ccggctcgta gaagcgcccg ttgccgtcgt
     1801 tgacgatgac cgagccgacg taggcccggt cggcgaggtt gtcgacccgc aggaactcgg
     1861 ccaggcgcca gcgaccggcg cgctgctcga agccgagcac ggcgttggcg acggtgaagc
     1921 tgcccgcgaa ctcgctgttg gcatcgttca ccgggacgcg cgagcggtgc agcagctcca
     1981 gggcggcgcg aaggcccaag ggcgcgtgcc gccaggcgcc ttcggcatag aactgcacct
     2041 cgggaacgcc cggcagctgg cgtcccgcgg cggcggtgcc gaaatccgac gcaaaagcgg
     2101 catccagcca cgtgtaggcg gcgcgcgcct cgaacgggcc ggcgagcaga gattccgccc
     2161 cgagctcgat gccccgccgc tcggtgcgcc cggcgttgcg gaacgtcgta cgcccgccgc
     2221 tcgactggtc gaccacgatc tcgtcacgcg tcgcgatgtc gaagaccgcc aggttgagcc
     2281 ggccgagtcc gggccgtacg gccttgaagc ccgcctccac gtgccggctg cgcgacggca
     2341 ccaggtcgaa gttgaggccg gtgccgacgc tgcggtacgc cagctcgacg aacgtgggcg
     2401 tctcgaaacc gcggccgtaa ttcgcataca ggctggtcag cgcgtcaagc cggtacagaa
     2461 tgcccgccac cggcgtgttc gcttcgaagg tcacgctgcc gctgtcgtcc gggttgccgg
     2521 cggcgatgaa gttgtccgcg gtcttgaact cgacgcggct gctgcgcagc cccgcatgcg
     2581 ccgaccagcg ctccgcgaag cgccactcgg cctgcacgta ggcgtcgctg gcgtgcacgg
     2641 cattgtcctc gtcgcgcttg agcgcgcccg cgacgccgtt gttgttgatg aagccgcggc
     2701 gccgttcgtc catgcgctcg tattccagcc ccgccgccag gcgcagcggc cgtccgcccg
     2761 ccgccatgtc gcgctgcccg cgcagcgcga tgccgccgaa gacccgctcc agatccacca
     2821 cgccgcccga agcggtgggc gcgctctgcg cggcaagcgg aatcgatagg tactgcacca
     2881 cgtcgcggcc gccgccccag gcgctcacct gcaggcgcgc tacgccttgg acctcgtgcc
     2941 ccagcgtcac cccgacctgg tcgtggtata cggactttcg cgtgttgaac gtcagcgccg
     3001 cgggcgcggc ctgccgcggg ttctgcgcca cctgcgccgc ggtcagcccg agcgggtcct
     3061 gcgtgtcggg ctggtgcagc gccatgccga ccagcgtgag cccggtaccc cgccccaacg
     3121 ccaggtcgag cttggtattg agctggtcgc gccgcaccgc gctgtgctcg cggtagccgt
     3181 cggattcgaa gcgcgacagg ttgacggcat gtccgacgcc ctgggcgcgt gcgccgaact
     3241 tcagcgctgc gcgccgtgtg ccgtagctcc cggcggtcag cgcgccctcc agcgtcggca
     3301 ccggcggcgc gtcctccgtc tgtacgagga tgacgccgcc cgaagcgttg ccgtaaagcg
     3361 ccgaaaacgg cccgcgcagt acctcgacgc ggccggccga ccccagatcg aacgtcgcgg
     3421 cctggccctg gccgtcgggc atgctggcgg gaatgtcgtc cgcgatcagg cgtatgccgc
     3481 gcaccccgaa gctggcgcgc gcgccgaatc cgcgcgaaga cacctgcagg tcctgcgcat
     3541 agttgtgcct gttctggacc acgacgccgg gcacgcggcc caggctttcc gacaggttga
     3601 cttgcaaccg gccttcgcgc acgtcttccg cgtagagccg gtcgatggcg gccgggatgt
     3661 cgaggctggg ccggtcgacg cgcgtcgccg tcaccaccac cggatcggat ggctgggcga
     3721 acgcgccgca cgcaaccacc gccagccatg ccaacgcaag ccgcatgcgt ttattatggc
     3781 ggcaaccatg ccgccccgcg gaagccgcct cgcgtgcacc ctgaagacca tcgacggctg
     3841 ccagggctcg ttcgacgtca cgcccggcga gcaaccgaac agcatcgccg cggtgggcga
     3901 aatcaactgg aacaaggcgc ctgggaagcc ggtgcaggaa ggcgccttca ccgtcatcgg
     3961 cgacctcggc atgaccgggc aggtgatcct cgtcaaccag taccagtggc gcgcgctgac
     4021 cgaggcgaag ctggagaagt tcttctacgc ggcgatgctc tggggcaaga gcccgttcaa
     4081 ggtgatcgag gacgcgcagc tgatcctcaa gcggggcaag tagcgcgccc ggcgctcagg
     4141 ccttttctag cgcgcccacc gggcaggaaa cgccggtgcc gccgagcccg cagtagcccc
     4201 cgggattctt cgccaggtac tgctggtggt agtcctcggc gtagtagaac tcgcccgcgg
     4261 gcttgatctc ggtggtgatc gccccgtacc ccttcgcctt cagcgccgcc tggaacgcgt
     4321 cgcgcgccgc ggtcgccgcc ttcgcctgcg cctcgtcgca ggtatagatc gccgagcggt
     4381 actgcgagcc gcggtcattg ccctggcgca tgccctgcgt cgggtcgtgg ccttcccaga
     4441 acaccttgag cagcgcctcg tagctcacgc gcttcgggtc gaacaccacc agcaccgcct
     4501 cggtgtggtt ggtgaagccg ctgcacacct cctcgtacgt cgggttgggc gtctcgccgc
     4561 cggcatagcc gactgccgtg ctgtacacgc cgggcgtctg ccagaagatg cgctcggcgc
     4621 cccagaagca gcccagcccg aactgcgcga gctgcaggcc ttgcggaaac ggcggcacca
     4681 tgcggttgcc gtttacggcg tgcgtttccg gcacagccat cttctccttg cgcccgggaa
     4741 gcgtgctgtc cttaggcagg agctgggtca tcttcttcat gaacgcgaac atgggcgttt
     4801 cctcccggag gctagaatta catcatgaaa atctgcatct tcggcgccgg cgcggtgggc
     4861 ggcgtgatgg ccggcgcgct gcaccgcgcc ggccacgacg tctcggtcgt ggcgcggggc
     4921 gcgaacctga cggcgatccg cgaacgcgga ttgcgcatcc gcgcgcaggc cagcggtgag
     4981 atcgccacgc acaaggtgcg cgccgaagcc gaccccgcga agctcgggcc gcaggactgc
     5041 gtcatcgtcg cgctcaaggc gcacagccta cccgaggcgg cgcgcgcggt gccggcgctc
     5101 gccgggcccg acaccaccgt cgtcacggcg atgaacggcg tgccctggtg gttcttcgac
     5161 cggctgcgct tcggcgaggg caagctgcgc ctggacagcc tcgaccccga cggcgcgctc
     5221 gcgcgcgcca tgccgaccga gcgcatcgtc ggctgcgtga tccatttcgc ggcctcgacg
     5281 ccggagcccg gcctggtcag ccacaacatg ggcaacaagc tcatcctcgg cgagcccggc
     5341 ggccgcaact ccgcccgcac ccaggcgatc gccggcgcgc ttgccgcggc cggtttcgat
     5401 tgcgtgcaga gcgatttcat cgagaaggat ttctgggtca agctcctcgg caacgtcagc
     5461 ttcaacccgg tttcggcgct caccctcgcc accgccgaca agctgatcgc cgacgccgag
     5521 atcaaggact acatggtgac catcatgcgc gaggtgctgg cgatcgggcg cgcggtgggc
     5581 gtcgacgccg acatcgaccc ggaggcgcgt atcgacatgg cgcgtcatct cgggcgcttc
     5641 aagacctcga tgctgcagga tatggaagcg ggcaagccgc tcgagatcga cggcctgctg
     5701 accggcacgc tggaagtggc gcagaaggcg ggcatcgccg ccccgttcac gcacagcctc
     5761 tgcggcctca tccgcctgcg cgcccaggcg accgggcagt atcgatgagg gcgctccttc
     5821 tcgcgctcct ggcggcgctg ccgctcgcgg ccgccgccag ctacgacggc ccggcgctcg
     5881 acgcctgccg cgcaatcggc gagcgcgcgc tgaagcggga cggcgccgac gtcaaggcgc
     5941 tcgcattcga caacgaccgg cacctgtatt tcgagcgcaa ggcgcgcaag ctcggcagcc
     6001 agccgatcgg cgccacgctc tcgggccacg gggcgatcgt gcgcgcggcc ggccccgcgg
     6061 tggagatcgt gttcctttgc ctgctggcga gcgaggggcg cgcgctctgg ttccactggc
     6121 tgccgcggcg cgacgcgccg gcgcttgccc agtgccgccg cagcacgggc gccggcgtct
     6181 gcctggaggc gctgctcgag ctcgcggagc gggatctgct ggaggtatcc gcgctgcgct
     6241 tccaggaatc gctggaagcc gacgccaggg ccggaaacga tgccgcctcg acggcctacc
     6301 gcagctcggc ggccgcctgg cgcacctatc gggacctgga atgcgcacgg cgcggcgccg
     6361 ccggcagcga cgcctggcgc gcgtgccgga tcgaccttac gcgctggcgc tatctcgagc
     6421 ttcagtgatc ctcgagccgc ggccagcggc cgcggcgcgc ggagccgagg tcgtagctgg
     6481 ccatgcgcca gcgccgcggc acgccggcgg cccgcacgcg gcgcaggatc tcggcgcttg
     6541 agtcgtgcgg cgactcgaaa aggtgcccga ccccggcgcg cgccaccgct gcctccggca
     6601 ggccgacatc gccgcgctcc accacggccc acacttc
//
LOCUS       DBNHKE010000245        12827 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1372012, whole genome shotgun sequence.
ACCESSION   DBNHKE010000245 DBNHKE010000000
VERSION     DBNHKE010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1372012"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /gene="mnmC"
                     /locus_tag="AC16O9_16675"
     CDS             complement(<1..1517)
                     /gene="mnmC"
                     /locus_tag="AC16O9_16675"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF002480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmC2/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC1"
                     /protein_id="HIR6518290.1"
                     /translation="MSAPLFPARLAFNAEGTPYSREFDDLYHSADGGAAQAHHVFLGG
                     NGLPARWRGARSFTILETGFGFGLSFLATWDAWREDPARSARLHYVSVEKHPFTAQDL
                     AVLHAPHAAFAALSEELRSAWPLLVPGMHRLEFENGRVVLTLAFGDAAELVPQLRLAA
                     DAIYLDGFAPAKNPDMWSLALLKSVARLAAPGATAATWSVASSVREGLRAAGFVSERR
                     RGFARKSEMLVARFVPARVLRPAPSLPGERSAVVVGAGIAGAAVCERLASSGWEVTLV
                     ERHRGLALEASGNHAGAFHPVLAPDDSPFARITRAAFTFLLEHWRQFDAIGASPEWKR
                     CGVLQLARDAREAASQRAALEALKPPLSYAQLLDARAASVSAGATLAAGGLWFQEGGW
                     IRPRSFARALITRSGARVLYGREAAALERAGPRWIVRDREGDEIAAAGAVVLANAADA
                     LHLAPMPQVRLRRVRGQLTYLPPLRGLKTVVLRGGMVLPPIDGVSVAGASFDLDD"
     gene            1567..3072
                     /locus_tag="AC16O9_16680"
     CDS             1567..3072
                     /locus_tag="AC16O9_16680"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6518291.1"
                     /translation="MNKPAELKLDTVPCWIGGKPVVPAGRMGEVYNPATGQVTKRVPY
                     CDAAAIDDAVKAASAALPEWRDASILRRARVMQKFLQLMQSHQKDIARLITAEHGKTL
                     PDAMGSVQRGIEVVEFVCGIPQLLKGEYSENVDTAVDTHTLRQPVGVCAGITPFNFPA
                     MVPMWMFPVAIACGNTFILKPSEKVPSASIRMAELFKEAGLPDGVFNVVHGDKAAVDA
                     ILAHPGIAAVSFVGSTPIAKYIYETCAKSGKRVQALGGAKNHAVVLPDADLEFAADAL
                     IGAGYGSAGERCMAISAVVAVGAAADPLVKLLAKKAQAIKIGPGDADGVDMGPLVTAQ
                     HRDKVAGYIEKGKREGAKPVLDGSKPPVKEGFFLGTTLFDQVKPEMEIYQHEIFGPVL
                     VVLRVDTLEEAIALVNRNPYANGTAIFTESGGAARRFENEVQVGMVGINVPIPVPVAF
                     YSFGGWKNSLFGDLHVHGMEGVKFYTRTKVVTTRWPHQDTPAPGLHMPTLG"
     gene            3082..3882
                     /locus_tag="AC16O9_16685"
     CDS             3082..3882
                     /locus_tag="AC16O9_16685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6518292.1"
                     /translation="MADRLKGKAALVLGAGSVGEGVGNGRAIAALFAREGARVAGADV
                     RLAAAEETARLIAKDGGKMLALEADVTQEADLQRTVDATLKAFGRIDILVNNVGGSIP
                     GDVETITPEGWRGQFEFNLHYVYHSMRIVAPLMAKQGGGAIVNISSIAGLRHLGNELA
                     AYAAAKAGLQQFSRVTAVRYAAQGVRVNTVIPGLMNTPLVAERLAKSQSGGDAAALIA
                     KRHARTPTGRMGDAWDVAWAVVFLASDEAKFVTGAELVVDGGLTATIR"
     gene            complement(3879..4526)
                     /locus_tag="AC16O9_16690"
     CDS             complement(3879..4526)
                     /locus_tag="AC16O9_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6518293.1"
                     /translation="MLKLDSVDVAIQSVQILRRVSLELPTGTMAGLIGRNGAGKTTLM
                     KSIMGILKVSGGTISFDGADLLASPTWKRTGMGIGYMPEDRRLIPDLTCAENLLLPSW
                     AAKRADGEARLEKVYRLIPELREFAPRKGSQLSGGQQKMVAIGRALMCGEKLLLLDEP
                     FEGVAPALAQRLVEVVANLKREGMSVILSESDLQHSERMVDRIIVIDRGAVSVRG"
     gene            complement(4519..5328)
                     /locus_tag="AC16O9_16695"
     CDS             complement(4519..5328)
                     /locus_tag="AC16O9_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6518294.1"
                     /translation="MAAILDAQGLNKSFGAVTAASDINAAIARDSVVGLIGTNGAGKT
                     TFINMITGYLKPDTGRIYYEGRDITQLEPREVTRIGICRSFQIPQLYASLTAYENMMI
                     GIGVVLRNAGRGGFFSRGRPSVPGYDQPAEEVAERLLERFGLAEYRDKNAQVLPGGIR
                     KLLDIALTMVARPKILLLDEPTSGVSAEEKFDIMQMIMKAVRAEGITVLFVEHDMEVV
                     SRFAERVLAFYDGRIIADAPPGEALNDPEVKRYVVGGELHRMDEEKARPRA"
     gene            complement(5330..6265)
                     /locus_tag="AC16O9_16700"
     CDS             complement(5330..6265)
                     /locus_tag="AC16O9_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6518295.1"
                     /translation="MRADRTPRTLALILVAILAVGPIAPQWTLFLLTVALAKGLVVLG
                     LMMLLRCGLVSFGQALYYCLGAYAAGMLGRLYEVSGVFTLMLTGMAASAVVAFVLGFL
                     LARYRSIFFGLLSLAISMILYGLLVKTEALGSSDGFNVLPSTLFGWRPADAWVRYSVL
                     AVAAGVAFIAALAVHRYLGTPLGRLAPAIKDNEIRVEYMGASARRAIHVKYVIAAALA
                     GLGGALAATTVGHIDPEMAYWTTSGEFIFITILAGTGSVIAPFLGALIFGIIQTVAFD
                     ISPNTWQMVLGASLLAVIVFLPQGLWSLFKRRKAA"
     gene            complement(6262..7158)
                     /locus_tag="AC16O9_16705"
     CDS             complement(6262..7158)
                     /locus_tag="AC16O9_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6518296.1"
                     /translation="MDQLVAVLIDGIIYSAWLFVAAVGLTLIYGVMKIVNVTHGSFYA
                     LGAYAAASITGWALAQGMAPSLSYAVLLGSALLVTLVVAPVIERGILRFMYVKDEVVI
                     LLITYALFLILEDTIKLIWGVSPYFIAEPYGLLGSFDVMDLTYPYYNLILVGVAIACG
                     FGLFWMLGHTRTGKLLVAVIHDTEVSRAMGINVERMYLFTFTFGSLLAAIGGAFTAPT
                     VSVVPGLGIEVIVLSFAVVVIGGLGSLPGAALGAVIVGLVRSATIHYLPQVELFVIYL
                     VMAMVLAFRPRGLFSIAEARKI"
     gene            complement(7215..8483)
                     /locus_tag="AC16O9_16710"
     CDS             complement(7215..8483)
                     /locus_tag="AC16O9_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6518297.1"
                     /translation="MKRAIAAAAAAALVPAFAAAQEAPIKIGVVSFLSGAAAGPFGVP
                     ARNGAEIMFEMLNAGKVPAPYATKGFGGAPLQMNLVDESGGTTTQVTEFRNMVQRDNV
                     DLVIGYISSGSCLGIAPVAEELKKLTVFFDCGTPRIFEEKSYKYVFRTASTATMDSVG
                     AALYVRDLKKGKISTIAGLNQNYAWGQDSWGDFELSMKKLVPGVKVATSQMPKLFAGQ
                     YNAEISTILGAKADVVHSSFWDGDLEAFILQAGPRGLFKRSTVILTTGETAMYKLADR
                     IPDGVVIGARGPYGPYAPDNALNKWFSETFQDRYGVPPNYAAYQMAQAILGTKAAWEK
                     AQAANGDKRPSQDQVIAAFERLTFESPAGKVEMAIGAGHQAITETAYGVTKKVKGKMT
                     VANVKRYPAEMVNPPDGIKAADWIANGFKK"
     gene            8675..9454
                     /locus_tag="AC16O9_16715"
     CDS             8675..9454
                     /locus_tag="AC16O9_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6518298.1"
                     /translation="MGHETILYQEDGPIGWLTLNRPDDGNMFTPQMCHEVRDCINAVR
                     RETRTRVLVITGAGDKFFCIGGRKEGMEDTLLYAGVLPTLEMYEAIDRLQKPVIASVN
                     GFAVGGGNVLQVMCDLTIAKESAVFRQVGPMMGSFDAGYGTWYLEDLVGKKKAKEMWM
                     LNPKLSAREALAIGLINRVVPDDQLRDETKKMALELAERGAFALASIKGAFNARHGGV
                     SGLARVAHDLLLREYLETEESHELSEAFRARRKPDPGKFGR"
     gene            9458..11074
                     /locus_tag="AC16O9_16720"
     CDS             9458..11074
                     /locus_tag="AC16O9_16720"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6518299.1"
                     /translation="MNTVLTRLNPQEAARHYASGAWRKDTFYSLLQQHAAARPGAYAL
                     RDARVRLTWKELLAWVDATAQALHLAGVRRGQRVAVWLPSRAESVVTLLACARNGYLC
                     VPSLHQNYTGAEIVQLLERTRAAALVMQAGYGAGGAAALPAMKVVFDVDGASFPAPGA
                     ACEVAAVADPDKIIYMAFTSGTTGVPKGVMHSDNTLLANGRAMVADWKHDHSTVLLSL
                     SPTSHHIGAVAIEQMMAAGFELVLKDPPAGMTPLDWALACRATYVMGVPTHAIDILAE
                     AKRRGLKALGAVRLFYMAGSPIPREVAQTFLGMGITPQNIYGMTENGSHQYTLPTDDA
                     QTIVETCGRACHGYETRIFSQENPDLEVPAGTVGEIGTRGALLMLGYFDNQATTEDSF
                     NKDGWFMSGDLGTLDARGCLRIVGRKKDLIIRGGHNIHPAKIEDLAHQHAAVQKAAAF
                     GIADDRLGEKVCLAVICREGERVTGEALLDHLHAVGLSKYDMPEYFLALDAFPLTASG
                     KILKRELQEWAKSGRIAPQAVRWREPAKEK"
     gene            11076..11792
                     /locus_tag="AC16O9_16725"
     CDS             11076..11792
                     /locus_tag="AC16O9_16725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6518300.1"
                     /translation="MSAPSRVIAVTGASRGIGAAIAAELLRRGFTVGALTRKAVGGDG
                     TLAVACDVNDEASVRTAFKTLAQKAGRIDGLVNNAGVHLYGRSAELPTARYEEVMNTN
                     ARSVFLCCREAYPYLARAGGTIVNIGSFFDKLGVKQNLAYCASKAAVGAITRCLAVEW
                     APKGIRVLDVAPGYIETELNAEAMQKGALRDFLAARVPTGGPGSVQDVARLVAALFAE
                     DIPYLTGETLYLDGGQGMAH"
     gene            11792..>12827
                     /locus_tag="AC16O9_16730"
     CDS             11792..>12827
                     /locus_tag="AC16O9_16730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6518301.1"
                     /translation="MKVFERLDGTLALEDEERMLVESVRTLARDVLAPRAEHYDRTAE
                     FPADNVKAINGLGLNAMFIPEAYGGHPVSYAAYLACVREISKACAATGIIWATNFHGI
                     KPLVDFGAEAQKTRLLPRIAQGGLVALAITEPSAGSDATGMKTRFRPEGDDIVIDGGK
                     TFITNGDVADLILLFGKWSEIDDDRKSISALILEKGVPGLSVVRTEDKMGIRASGTAT
                     LAFDGCRVPRANLLGAPGDGLKLLLASLNKSRPSVAAHALGIARAAFEDALAYINERR
                     QSGRRIVEFQGVQFLIADLAAELAQCEAWLWHVARLVDAGAQDIGIEASMLKLRASDL
                     AMRNATQALPL"
BASE COUNT         2132 a         4357 c         4445 g         1893 t
ORIGIN      
        1 tcgtcgtcca gatcgaagct cgcgcccgcg accgagacgc cgtcgatcgg cggcagcacc
       61 atgcccccgc gtagcacgac ggtcttcagg ccgcgcagcg gcggcaggta ggtgagctgg
      121 ccgcgcacgc gccgcagcct gacctgcggc atgggcgcga gatgcagcgc atcggcggcg
      181 ttggcgagca ccacggcgcc cgccgcggcg atctcgtcgc cctcgcggtc gcgcacgatc
      241 cagcgcggcc ccgcccgctc cagcgctgcc gcctcgcgcc cgtagagcac gcgcgcgccg
      301 gagcgcgtga tcagcgcccg cgcgaagctg cgcggccgga tccagcctcc ttcctggaac
      361 cataggccgc ctgcggcgag cgttgcgccc gcgctgacgc tggccgcgcg cgcatcgagc
      421 agctgcgcat agctgagcgg aggcttgagc gcctcgagcg cggcgcgctg cgacgctgcc
      481 tcgcgcgcgt cgcgcgcgag ctgcagcacg ccgcagcgct tccattccgg tgatgcgccg
      541 atcgcatcga actggcgcca gtgctcgagc aggaacgtga aggccgcgcg cgtgatgcgc
      601 gcgaacggac tgtcgtcggg cgccagcacg gggtgaaagg cgcccgcgtg attccccgag
      661 gcctcgagcg ctagcccgcg gtgccgctcg accagcgtca cctcccagcc gctcgaggcg
      721 aggcgctcgc acaccgccgc cccggcaatg ccggcgccga cgacgacggc gctcctttct
      781 ccgggcagcg acggcgcggg tcgcagcacc cgcgccggca cgaaacgcgc gaccagcatt
      841 tcgctcttcc tggcgaaacc acggcgcctc tcggacacaa agcccgccgc gcgcaagccc
      901 tcgcgcacgc tggatgccac actccaggtg gccgccgtag cgccgggcgc ggcgaggcgc
      961 gctaccgact tcagcagcgc gagcgaccac atgtcggggt tcttcgccgg cgcgaagcca
     1021 tcgaggtaga tcgcgtccgc ggcgagccgc aactgcggca cgagctccgc ggcgtcgccg
     1081 aaggcgagcg tgagcacgac acgcccgttc tcgaactcga gccggtgcat cccggggacc
     1141 agcagcggcc aggcgctgcg caactcctcg gagagcgcgg cgaacgccgc gtgcggcgcg
     1201 tgcagcaccg cgaggtcttg cgccgtgaaa ggatgctttt ccaccgagac gtaatgcagg
     1261 cgcgccgagc gcgcgggatc ctcacgccac gcgtcccagg tggcgaggaa gctgaggccg
     1321 aaaccgaagc cggtctcgag gatggtgaag cttcgcgcac cgcgccagcg cgccggcaag
     1381 ccgttgccgc cgaggaacac gtggtgcgct tgcgccgcgc cgccgtcggc gctgtggtac
     1441 aggtcgtcga actcgcggct gtacggggtg ccctcggcgt tgaaggcaag gcgggcggga
     1501 aagagggggg cggacacgcg ctgagtatat cggcgtaaaa ttttctcctg tacgaggagt
     1561 ccgccgatga acaagcccgc cgaactgaag ctcgacaccg tgccctgctg gatcgggggc
     1621 aagcccgtgg tgcccgccgg acgcatgggg gaggtgtaca accccgcgac cgggcaggtg
     1681 acgaagcgcg tgccctactg cgacgccgcg gcaattgacg acgcggtgaa ggccgcctcc
     1741 gccgcgctgc cggagtggcg tgacgcttcc atcctgaggc gcgcgcgcgt catgcagaag
     1801 ttccttcagc tgatgcagtc gcaccagaag gacatcgcgc gcctgatcac cgcggagcac
     1861 ggcaagacct tgcccgacgc gatgggctcg gtgcagcgcg gcatcgaagt ggtggagttc
     1921 gtctgcggca tcccgcagct tctgaaaggc gagtactcgg agaacgtcga cacggcggtg
     1981 gacacgcaca ccctgcgcca gccggtcggg gtgtgcgcgg gcatcacgcc gttcaatttt
     2041 ccggccatgg tgccgatgtg gatgttcccg gtggcgatcg cctgcggcaa cacgttcatc
     2101 ctcaagccct cggagaaggt gccctcggcg tcgatccgca tggcggagct tttcaaggag
     2161 gcagggctgc ccgacggcgt gttcaacgtg gtgcacggcg acaaggcggc ggtggacgcg
     2221 atcctcgcgc acccgggaat cgcggcggtc tccttcgtcg gctcgacgcc gatcgccaaa
     2281 tacatctacg agacctgtgc gaagagcggc aagcgggtgc aggcgctcgg cggcgcgaag
     2341 aaccacgccg tggtgctgcc cgacgccgac ctcgagttcg ccgccgacgc gttgatcggc
     2401 gcgggttacg gctcggcggg ggagcgctgt atggcgatct cagccgtggt ggcggtgggc
     2461 gcagcggccg acccgctcgt caagctgctg gcgaaaaagg cgcaggcgat caagatcggc
     2521 ccgggcgatg ccgacggcgt cgacatgggg ccgctcgtca cggcgcagca tcgcgacaag
     2581 gtcgcgggct acatcgagaa gggcaagcgc gaaggcgcga agccggtgct cgacggcagc
     2641 aagccgccag tgaaggaagg gttcttcctc ggcaccacgc tattcgacca ggtgaagccg
     2701 gagatggaga tctaccagca cgagatcttc ggcccggtgc tggttgtgct gcgcgtcgat
     2761 acgctggagg aggcaattgc gctggtgaac cgcaacccct atgccaacgg cacggcgatc
     2821 ttcaccgaat cgggcggcgc cgcgcggcgc ttcgagaacg aggtgcaggt gggcatggtc
     2881 ggcatcaacg tgccgatccc ggtgccggtg gcgttctact ccttcggcgg ctggaagaac
     2941 tcgctgttcg gcgacctgca cgtgcatggc atggagggcg tcaagttcta tacccgcacc
     3001 aaggtcgtca ccacgcgctg gccgcaccag gacacgccgg ctccgggctt gcacatgccg
     3061 acgctgggct gaaaggcccc aatggcggac cggctgaagg gcaaggcggc gctcgtcctc
     3121 ggcgcgggct cggtgggcga gggtgtcggc aacgggcgcg ccatcgcggc gctcttcgcg
     3181 cgcgaaggcg cgcgcgtcgc gggcgccgat gtgcggctcg cggcggccga ggaaacggcg
     3241 cggctcatcg ccaaggatgg cggcaagatg ctggcgctcg aggccgacgt cacccaagag
     3301 gccgacctgc agcgcacggt ggacgcaacc ttgaaggcgt tcgggcgcat cgacatcctg
     3361 gtgaacaacg tcggcggcag catcccgggc gacgtggaga cgatcacgcc cgagggctgg
     3421 cgcgggcagt tcgagttcaa tctgcactac gtgtaccact cgatgcgcat cgtggcgccg
     3481 ctgatggcga agcagggcgg cggggccatc gtcaatattt cctcgatcgc gggattacgc
     3541 catctgggca acgagctcgc cgcgtacgcg gcggcaaagg ccgggctgca acaattctcg
     3601 cgcgtcaccg cggtgcgcta cgcggcgcag ggtgtgcgcg tcaataccgt gattccggga
     3661 ctgatgaaca cgccgctggt ggccgagcgt ctcgcgaagt cgcagtccgg gggcgatgcg
     3721 gcggcgttga tcgccaagcg ccacgcgcgc acgccgacgg ggcgcatggg cgacgcctgg
     3781 gacgtggcct gggcggtcgt gttcctcgct tcggacgagg cgaagttcgt caccggcgcc
     3841 gagctcgtcg tcgacggggg gctgacggct acgatccgct agccgcgcac gctgaccgcg
     3901 ccgcggtcga tgacgatgat gcggtcgacc atgcgctccg aatgctgcag gtcggactcc
     3961 gacaggatca ccgacatgcc ttcgcgcttg aggttcgcca ccacctccac cagccgctgc
     4021 gccagtgccg gcgcgacgcc ctcgaacggc tcgtcgagca gcagcagctt ctcgccgcac
     4081 atcagcgcgc ggccgatggc caccatcttc tgctgcccgc cggagagctg cgagcccttg
     4141 cgcggcgcga actcgcgcag ctccgggatc agccggtaga ccttctccag ccgcgcctcg
     4201 ccgtcggcgc gcttcgccgc ccaggacggc agcagcaggt tctccgcgca ggtcaggtcc
     4261 gggatcaggc gccggtcctc gggcatgtag ccgatgccca tgccggtgcg cttccaggtc
     4321 ggcgaggcga ggagatccgc gccgtcgaag ctgatcgtgc cgcccgagac cttgagaatc
     4381 cccatgatgc tcttcatgag cgtggtcttg ccggcgccgt tgcggccaat caggcccgcc
     4441 atggtgccgg tgggcagctc gagcgacacg cggcgcagga tctgcaccga ctggatggca
     4501 acatcgacgc tgtcgagctt aagcacgcgg tcgtgccttc tcttcgtcca tgcggtgcag
     4561 ctcgccgccg accacgtagc gcttcacctc cgggtcgttc agcgcctcac cgggcggcgc
     4621 gtcggcgatg atccgaccgt cgtagaaggc gagcacgcgc tccgcgaagc ggctcaccac
     4681 ctccatgtcg tgctcgacga acagcacggt gatgccttcg gcacgcaccg ccttcatgat
     4741 catctgcatg atgtcgaact tttcctcggc cgagacgccg ctggttggct cgtcgagcag
     4801 caggatcttg ggccgcgcca ccatggtgag cgcgatgtcg agcagcttgc ggatcccgcc
     4861 cgggagcacc tgcgcgttct tgtcgcggta ctcggcgagg ccgaagcgct cgagcaggcg
     4921 ctcggcgacc tcctccgccg gctggtcgta gcccggcacg ctcggccggc cgcgcgagaa
     4981 gaagcctccg cgcccggcgt tgcgcagcac cacgccgatg ccgatcatca tgttctcgta
     5041 cgcagtgagc gaggcgtaga gctgcggaat ctggaacgag cggcagatgc cgatgcgggt
     5101 gacctcgcgc ggctcgagct gcgtgatgtc gcggccctcg tagtagatgc ggccggtgtc
     5161 gggcttgagg tacccggtga tcatgttgat gaaggtggtc ttgccggcgc cgttggtgcc
     5221 gatcaggccg accaccgagt cgcgtgcgat ggcggcgttg atgtcgctgg ccgcggtgac
     5281 tgcgccgaag ctcttgttca ggccctgcgc gtcgaggatc gccgccatct caggccgcct
     5341 tccggcgctt gaacagcgac cacaggccct gcggcaggaa cacgatgacc gccagcaggg
     5401 atgcgccgag caccatctgc caggtgttgg gcgagatgtc gaacgccacc gtctggatga
     5461 tgccgaagat gagcgcgccc aggaacggcg cgatcacgct gccggtgccg gcgagaatcg
     5521 tgatgaagat gaattcgccc gaggtggtcc agtaggccat ctccgggtcg atgtggccga
     5581 cggtggtggc cgcgagcgca ccgcccaggc ctgcgagcgc cgcggcgatg acgtacttga
     5641 cgtggatcgc gcggcgcgcc gaggcgccca tgtactcgac gcggatctcg ttgtccttga
     5701 ttgccggcgc aaggcgtccg agcggtgtgc cgaggtagcg atgcaccgcc agcgcggcga
     5761 tgaaggccac cccggcggcc acggcgagca cgctatagcg cacccatgcg tcggcggggc
     5821 gccagccgaa gagcgtcgag ggcagcacgt tgaacccgtc gctcgagccc agcgcttccg
     5881 tcttcacgag cagcccgtaa aggatcatcg agatcgcgag gctgagcagc ccgaagaaga
     5941 tgctgcggta gcgcgcgagc agaaatccca ggacgaaggc gaccacggcc gaagccgcca
     6001 tgccggtgag catgagcgtg aacacgccgc tcacctcgta caggcgcccg agcatgcccg
     6061 cggcataggc gccgaggcag taatagagcg cctggccgaa ggacaccagg ccgcagcgca
     6121 gcagcatcat gaggccgagc accacgaggc ccttggcgag cgcaacggtg agcaggaaca
     6181 gcgtccattg cggcgcgatc gggcccacgg cgaggatcgc gacgaggatc agcgccagtg
     6241 tacgcggcgt gcggtcggcc ctcatatctt tcgcgcctcg gcgatgctga acaggccgcg
     6301 cggccggaac gcgagcacca tcgccatcac gagatagatg acgaagagct cgacctgcgg
     6361 caggtaatgg atggtggccg agcgcaccag gccgacgatc accgcgccga gcgcggcgcc
     6421 gggcaggctg ccgaggcctc cgattaccac caccgcgaac gagagcacga tcacctcgat
     6481 gcccaggccc ggcaccaccg acaccgtggg cgcggtgaag gcgccgccga tcgcggccag
     6541 cagcgagccg aaggtgaagg tgaacaggta catgcgctcg acgttgatgc ccatcgcgcg
     6601 gctcacctcg gtatcgtgga tcaccgccac cagcagcttg ccggtgcgcg tgtggccgag
     6661 catccagaac aggccgaagc cgcacgcgat cgccacgccg accaggatca ggttgtagta
     6721 cgggtaggtc aggtccatca cgtcgaagct gcccagcagc ccgtagggct cggcgatgaa
     6781 atacgggctc acgccccaga tcagcttgat ggtgtcttcc agaatcagga acagcgcgta
     6841 ggtgatgagc aggatcacca cctcgtcctt cacgtacatg aagcgcagga tgccgcgctc
     6901 gatgaccggc gccacgacca gcgtcaccag cagcgccgag ccgagcagca ccgcgtagga
     6961 gagcgaaggc gccatgccct gcgcgagcgc ccagccggtg atcgaagcgg ccgcataggc
     7021 gccaagcgcg tagaagctgc cgtgcgtcac gttgacgatc ttcatgacgc cgtagatcag
     7081 cgtcagtccg accgcggcaa cgaacagcca cgcggagtag atgatgccgt cgatcaggac
     7141 ggcgacgagt tggtccacgc tcccccggga cgaaaaacgg ctgccctgcg gcagccgttg
     7201 ttcagctcaa acgcctactt cttgaagccg ttggcaatcc agtccgcggc cttgatgcca
     7261 tcgggtggat tgaccatctc ggccgggtag cgcttgacgt tcgccaccgt catcttgcct
     7321 ttcaccttct tggtcacgcc gtaggccgtc tcggtgatcg cctggtggcc ggcgccgatc
     7381 gccatctcca ccttgcccgc ggggctctcg aaggtcaggc gctcgaaagc cgcgatcacc
     7441 tggtcctggc tcgggcgctt gtcgccgttc gcggcctgcg ctttctccca cgccgccttg
     7501 gtgccgagga tcgcctgcgc catctggtag gcggcgtagt tcggcggcac gccgtagcgg
     7561 tcctggaagg tctccgagaa ccacttgttc agcgcgttat ccggcgcata ggggccgtag
     7621 ggcccgcgcg cgccgatcac cacgccgtcc ggaatgcggt cggcgagttt gtacatcgcg
     7681 gtctcgccgg tggtgaggat cacggtgctc ctcttgaaca ggccgcgcgg tcccgcctgc
     7741 aggatgaacg cctccaggtc gccgtcccag aagctcgagt gcaccacgtc cgccttggcg
     7801 ccgagaatcg tcgagatctc ggcgttgtac tggccggcga agagcttggg catctgcgag
     7861 gtcgcgacct tcacgccggg gacgagcttt ttcatcgata gctcgaagtc gccccaggag
     7921 tcctggcccc aggcatagtt ctggttcagg ccggcgatgg tgctgatctt gcccttcttc
     7981 aggtcacgca cgtagagcgc cgcgccgacg ctgtccatgg tcgcggtgga agccgtgcga
     8041 aacacgtact tgtagctctt ctcttcgaag atgcgcggcg tgccgcagtc gaagaacacg
     8101 gtgagcttct tcagctcttc ggccaccggc gcgatgccga ggcagctgcc gctcgagatg
     8161 tagccgatga cgaggtccac gttgtcccgc tgcaccatgt tgcggaactc ggtcacctga
     8221 gtggtggtgc cgcccgactc gtcgaccagg ttcatttgca gcggcgcgcc accgaagccc
     8281 ttggtggcgt agggcgccgg caccttgccg gcgttcagca tctcgaacat gatctccgcg
     8341 ccattgcgcg ccgggacgcc gaacggccct gcggccgcgc cggacaggaa cgagacgacg
     8401 ccgatcttga tcggcgcttc ctgggcggcg gcaaacgccg gcaccagcgc ggcggccgcg
     8461 gccgcggcaa tagccctctt catgacgtgc atggattcct cctccttgat ggccggaaga
     8521 tggtgcagca aaacggcacc gacggtcaag caagcgcggc gatcgtctcg cggctcgaac
     8581 tgcaggcgtg cgtgcgcgcg cggcggcggc ggccattcaa attcgcgtgc cgaacgacta
     8641 tcatcgcctg gcgcaatccg cagaggaaac tcccgtgggt catgaaacca ttctctacca
     8701 agaggacggg ccgatcggct ggctcaccct caaccgtccc gacgacggca acatgttcac
     8761 gccgcagatg tgccatgagg tgcgcgactg catcaacgcg gtgcggcgcg agacgcgcac
     8821 ccgcgtgctc gtcatcaccg gcgcgggcga caagttcttc tgcatcggcg gcaggaagga
     8881 gggcatggag gacacgctgc tctatgccgg ggtgctcccc acgctcgaga tgtacgaggc
     8941 gatcgaccgg ctgcaaaagc ccgtcatcgc ctcggtgaac ggctttgcgg tgggcggtgg
     9001 caacgtgctg caggtgatgt gcgacctgac gatcgccaag gaaagcgcgg tgttccgcca
     9061 ggtcgggccg atgatgggca gcttcgacgc cggctacggc acctggtacc tcgaggatct
     9121 cgtcggcaag aaaaaggcca aggagatgtg gatgctgaac ccgaaattga gcgcgcgcga
     9181 ggcgctcgcc atcgggctca tcaaccgcgt ggtgcccgac gaccagctgc gcgacgagac
     9241 gaagaagatg gcgctggagc tcgccgagcg cggcgcgttc gccctcgcct cgatcaaggg
     9301 cgccttcaac gcccgccacg gcggggtctc gggcctcgcg cgcgtggcgc atgacctctt
     9361 gctgcgcgag tacctcgaga ccgaggagtc gcacgagctc tcggaggcct tccgcgccag
     9421 gcgcaagccc gacccgggca agttcggccg ctagggcatg aacacggtcc tgacgcgcct
     9481 caacccgcag gaggcggcgc gccattacgc ctcgggggcg tggcgcaagg acacgttcta
     9541 ttcgctgttg cagcagcacg ccgccgcgcg cccgggggct tacgcgctgc gcgacgcgcg
     9601 cgtgcgattg acatggaagg aactcctcgc ctgggtcgac gcgacggcgc aggcgctgca
     9661 ccttgccggc gtgcgccgcg gccagcgcgt cgccgtctgg ctgccctcgc gcgccgagag
     9721 cgtggtgacg ctgctggcct gcgccaggaa cggatacctc tgcgtcccct cgctgcacca
     9781 gaactacacc ggcgccgaga tcgtgcaact cctcgagcgc acgcgcgccg cggcgctggt
     9841 gatgcaggcg ggctatggcg ccggcggcgc ggcggcactg cccgcgatga aagtggtatt
     9901 cgacgtcgac ggcgcgtcgt tcccggcgcc gggcgcggcc tgcgaggtgg cggccgtcgc
     9961 cgatcccgac aagatcatct acatggcctt cacctcgggc accaccggcg tgcccaaagg
    10021 cgtgatgcac tccgacaata cgctgctcgc caacggccgc gccatggtcg ccgactggaa
    10081 gcacgatcat tcgacggtgc tgctcagcct gtcgccgacg agccaccaca tcggcgccgt
    10141 cgcgatcgag cagatgatgg ccgcgggctt cgagctcgtg ctgaaggacc cgcccgcggg
    10201 catgacgccg ctcgactggg cgcttgcctg ccgcgccacc tacgtgatgg gcgtgccgac
    10261 gcacgccatc gacatcctcg ccgaggccaa gcgccgcggc ttgaaggcgc tcggcgcggt
    10321 gcgcctgttc tacatggcgg gctcgccgat cccgcgcgag gtggcgcaga ccttcctcgg
    10381 catgggcatc acgccgcaga acatctacgg catgaccgag aacggctcgc accagtacac
    10441 cctgcccacg gacgacgcgc agaccatcgt cgagacctgc gggcgcgcct gccacggcta
    10501 tgagacgcgc atcttcagcc aggaaaatcc cgacctggag gtgccggcgg gcacggtcgg
    10561 cgagatcggc acgcgcggcg cgctccttat gctcggctat ttcgacaacc aggcgaccac
    10621 cgaggattcg ttcaacaagg acggctggtt catgtccggg gacctcggca cgctcgacgc
    10681 gcgcggctgc ctgaggatcg tcggccgaaa gaaagacctc atcatccgcg gcgggcacaa
    10741 catccacccg gcgaagatcg aggacctggc gcaccagcat gcggcggtgc agaaggcggc
    10801 ggcgttcggc atcgccgacg atcgcctcgg cgagaaggtc tgccttgcgg taatctgccg
    10861 cgagggcgag cgcgtgaccg gggaagcctt gctggatcac ctgcacgcgg tcggcctttc
    10921 caagtacgac atgcccgaat acttcctcgc cctggacgcc ttcccgctca ccgcgagcgg
    10981 caagatcctg aagcgcgagc tgcaggaatg ggcgaagagc ggccgcatcg cgccgcaagc
    11041 cgtgcgctgg cgcgagcccg caaaggagaa atgaggtgtc cgcgccaagc cgtgtcatcg
    11101 cggtcaccgg cgcgagccgc ggcatcggcg cggcgatcgc cgcggagctg ctgcgccggg
    11161 gattcacggt gggcgcgctg acccggaagg ccgtcggcgg cgacggcacg ctggccgtgg
    11221 cctgcgacgt caacgacgaa gcatccgtca ggacggcgtt caagacgctc gcgcagaagg
    11281 ccgggcgcat cgatgggctg gtgaacaacg ccggcgtgca cctctacggc cgctcggcgg
    11341 agctgccgac cgcaagatac gaagaggtga tgaacaccaa cgcacgctcg gtgttcctgt
    11401 gctgccgcga ggcctatccg tacctggcgc gcgcgggcgg caccatcgtc aacatcggct
    11461 cgttcttcga caagctcggc gtgaagcaga acctcgccta ttgcgcctcc aaggccgcgg
    11521 tcggcgccat cacgcgctgc ctcgcggtcg aatgggcgcc gaagggaatt cgcgtgctcg
    11581 acgttgcccc cggctacatc gagaccgagc tcaacgccga ggcgatgcag aagggcgcgc
    11641 tgcgcgactt cctcgcggcg cgcgtgccga ccggcgggcc ggggagcgtg caggacgtcg
    11701 cgcgcctggt ggcggcgctc ttcgccgagg acattccgta cctcaccggc gagaccctct
    11761 acctcgacgg cggccagggg atggcccatt gatgaaggtg tttgagcgcc tcgatggcac
    11821 gctcgcgctc gaggacgagg agcgcatgct ggtggagagc gtgcgcacgc tcgcgcgcga
    11881 cgtcctcgcg ccgcgcgccg agcactacga ccgcacggcc gagttcccgg cagacaacgt
    11941 taaggcgatc aacggcctcg gcctgaacgc gatgttcatc cccgaggcct atggcgggca
    12001 cccggtgtcc tacgcggcgt atctcgcctg cgtgcgcgag atcagcaagg cctgcgcagc
    12061 caccggcatc atctgggcga ccaacttcca cggcatcaag ccgctggtcg acttcggcgc
    12121 cgaggcgcag aagacgcgac tgctgccgag gatcgcgcag ggcgggctcg tggcgctcgc
    12181 catcaccgag ccaagcgccg gatccgacgc caccggcatg aagacgcgct tccggcccga
    12241 gggcgacgac atcgtcatcg acggcggcaa gaccttcatc accaatggcg atgtcgccga
    12301 cctgatcctg ctgttcggca agtggagcga gatcgacgac gaccggaaat cgatctcggc
    12361 gctgattctg gaaaagggcg tgccgggcct gtcggtcgtg cgcaccgagg acaagatggg
    12421 gatccgcgcc tcgggcaccg cgacgctcgc cttcgacggc tgccgcgtgc cgcgtgcgaa
    12481 cctgctcggc gcgccgggcg acggcctcaa gctgctgctc gcctcgctca acaagtcgcg
    12541 cccgagcgtc gccgcgcacg cgctcggcat cgcgcgcgcg gcgttcgagg acgcgctcgc
    12601 ctacatcaac gagcggcgcc agtcgggccg gcgcatcgtc gagttccagg gcgtgcagtt
    12661 cctgatcgcg gacctcgccg ccgagctcgc gcagtgcgag gcctggctgt ggcacgtcgc
    12721 gcgcctggtg gacgccggcg cgcaggacat cggcatcgag gcctcgatgc tcaagctccg
    12781 tgcctccgac ctcgcgatgc gcaacgcgac ccaagccctg ccgctgc
//
LOCUS       DBNHKE010000246         5043 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_894557, whole genome shotgun sequence.
ACCESSION   DBNHKE010000246 DBNHKE010000000
VERSION     DBNHKE010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_894557"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..379
                     /locus_tag="AC16O9_16735"
     CDS             41..379
                     /locus_tag="AC16O9_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518302.1"
                     /translation="MSTNAYRAALVQTASLAIAALQCDLIGTTDLSTKGHRRVLQQLL
                     GGLGEYRHAEEKGVAPRQFAPGEWALRIGEVAGDVAGESAPDVRLASTLFQVVLSGSM
                     CRAVLDNQEG"
     gene            382..1299
                     /locus_tag="AC16O9_16740"
     CDS             382..1299
                     /locus_tag="AC16O9_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518303.1"
                     /translation="MPYKKHEDQLACERRYREKRRAEWLKVKGDGPSPVANQYARRIG
                     NVLDLGIPVPKHGLKIAVLPDTQVMPGVPMEHLGWYGAYIAEKKPDVIVCLGDFGDYP
                     SLSVFGRGERDFAAHRYSKDIAAFHEGMQQFLGPIEAEKARDKSWRPWMEFIEGNHEW
                     HIDRITQQFPFLEGTIDKSDLCLPEYGWRVHPFLQPVAIGGVAFCHYFPSGVMGRPIT
                     SAPQILSKLHMSAVAGHQQGREIAFSRRADGGSMTAIISGSFYQHNYKYLSPFTNAHW
                     RGAWFLHEVKDGHFDEMPLSLEYLRRRYA"
     gene            1296..1529
                     /locus_tag="AC16O9_16745"
     CDS             1296..1529
                     /locus_tag="AC16O9_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518304.1"
                     /translation="MNASEMDRETLAAVFASRVALAKRTPEQAQADALLEHQRTEAWV
                     KSPSTKEASFLWFCDEFDLDAGAVRKAIRERRG"
     gene            1531..2751
                     /locus_tag="AC16O9_16750"
     CDS             1531..2751
                     /locus_tag="AC16O9_16750"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIR6518305.1"
                     /translation="MPEPRPYQIEGRDFLAARRHALLADEMRVGKTPQAILAAHKAGA
                     RSMLVVCPAVAVLQWEREIERWWPSGPLPRTRVWSYDKARANWVMGTRGAVDVFIPDE
                     CHFAGNPEAARTRMVYAKHSFAWSAGATWPLSGTPAPKSAASLWPMLRAFGIVDMTYE
                     DFLTRYCTLRPMSWVPTGTKVKTAPELNAMLAKIMLRRTRRQVRPDMPDIGFNFLEVK
                     PETSASYVVPPGLSDAALLEWMALNEHANREGRVEVALAKGGPLAEEIAFAINNGLLK
                     QTVVFGWHIAPLEWLVGTLNARGIRAAAITGKTPRTERERIQNEFRAGRLDVVMANIL
                     AAGTAIDLSAARHGYFLEMDWVPGNNVQAANRLVSLDRSEAVTFDVVTWPGSADDRVQ
                     NVLLTRVAQLAKLY"
     gene            2766..3311
                     /locus_tag="AC16O9_16755"
     CDS             2766..3311
                     /locus_tag="AC16O9_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518306.1"
                     /translation="MVKITMEFPTLDAAIVALGKLSSAADTTIVDGKPRRGRSDKGLP
                     RKSRSAPTESNAKGEGAGAQSEGAAEATVASAATPAAAPAPAASQVAAAAGTKDGGTS
                     TNAEPAAKAPDVAGAKPATAEDAQKAIEKLFDAPPPVGGLEQARIVMSRFGVKRVRDL
                     QEDQRAEFIALAEKVLAGGAA"
     gene            3308..3433
                     /locus_tag="AC16O9_16760"
     CDS             3308..3433
                     /locus_tag="AC16O9_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518307.1"
                     /translation="MNPGFYSLTCILSAALAAAKDPVDRAALFVAINGVANEENQ"
     gene            3430..4638
                     /locus_tag="AC16O9_16765"
     CDS             3430..4638
                     /locus_tag="AC16O9_16765"
                     /inference="COORDINATES: protein motif:HMM:NF022379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HIR6518308.1"
                     /translation="MSGVHARLSASAAHRWLKCPPSVNAKGGGSSIHAATGTFAHDIA
                     ARCLNDPSISASDFFLKKEKVDGFEVECDLEMVDAVNLYLDEIAADLQPGDLTWVEMP
                     LLQALSRIDPDLGGTADYVRYRPATRQLLVMDFKYGSGVYVEADDNDQLKLYAVGAML
                     ECGRSVNEVTVGIVQPRFEGAKPVRTSVFKGHELLEFVADVQEAANKSRDPNAPLSAG
                     NHCKFCPLARTCPELERRHHELVAADFGVVGDATVAPTAAAPGALVDYRALAVALDSV
                     PLVKERIKAIEEFAYQEALKGAEIPGYKLVDKRPVRRWKSEGEVILWAEAQAIDPYAP
                     RSVVSPAQLEKKLAESAPKGKKKEAGKVLEPFVERVSSGTALVAATDARPPAKRVTAE
                     DFAVIAEDRG"
     gene            4680..>5043
                     /locus_tag="AC16O9_16770"
     CDS             4680..>5043
                     /locus_tag="AC16O9_16770"
                     /inference="COORDINATES: protein motif:HMM:NF022444.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ssDNA-binding protein"
                     /protein_id="HIR6518309.1"
                     /translation="MAKVTLKDVRLTFPQLFEPKQVNGQGDPKFSGAFLFAKNHPQVA
                     DIKAAIVEAATAKWGAKHAEVLKALYAGDKLCLHDGDAKSDYDGYAGNLFINASNKIR
                     PLVIDGQRAPLVAADGKPY"
BASE COUNT          892 a         1651 c         1767 g          733 t
ORIGIN      
        1 cgcccccgcc gctcgaggcg cccgaagggg cgaactgccc gtgagcacta acgcctaccg
       61 cgcggccctc gtgcagactg cctcgctcgc catagccgcg ctacagtgcg atttgatcgg
      121 cactaccgac ttgtcgacga agggccaccg gcgcgtgctg cagcagctgc tgggcggcct
      181 cggggagtat cgccacgcag aagagaaggg tgtcgcgccg cgccagttcg cgcccggaga
      241 gtgggcgctg cgcatcgggg aggtggcggg agacgtggcc ggcgagtcgg caccggacgt
      301 ccggctggcc tcgacgctgt ttcaggtggt gctgagcggc agcatgtgcc gcgcggtact
      361 cgacaaccag gagggctagc cgtgccgtac aagaaacacg aggaccagtt ggcgtgcgag
      421 cgccgataca gggagaagcg ccgcgccgag tggctgaagg tgaagggcga cggcccctcg
      481 ccggtcgcca accagtacgc gcgccgcatt ggcaacgtgc tagatcttgg catacccgtg
      541 ccaaaacatg gcctgaagat cgccgtgctg cctgacaccc aggttatgcc cggcgtgccg
      601 atggagcacc tcggctggta cggggcgtac atcgccgaga aaaagccgga cgtcattgtc
      661 tgcctcgggg atttcggcga ctacccgtcg ctctcggtgt tcggccgcgg cgagcgcgat
      721 ttcgccgcgc accgctactc gaaggacatc gcggctttcc acgagggcat gcagcagttc
      781 ctcgggccga tcgaggccga gaaggcgcgc gacaagtcct ggcgcccgtg gatggagttc
      841 atcgaaggca accacgagtg gcatatcgac cggatcacgc agcagttccc gttcctcgag
      901 ggcacgatcg acaagtccga cctgtgcctg cccgagtacg gctggcgcgt gcacccgttc
      961 ctgcagccgg tggcgatcgg cggggtggct ttctgccact acttcccctc aggggtgatg
     1021 ggccgcccga tcacgagcgc cccgcagatc ctctcgaagc tgcacatgag cgccgtcgcc
     1081 gggcaccagc aggggcgcga gattgcgttc tcgcgccggg ctgacggcgg cagcatgacg
     1141 gcgatcatct ccgggtcgtt ctatcagcac aactacaagt acctgtcgcc cttcaccaac
     1201 gcccactggc gcggcgcgtg gttcctgcac gaagtgaagg acgggcactt cgacgagatg
     1261 cccctgtcgc tcgagtacct gcgccggagg tacgcgtgaa cgcaagcgag atggaccggg
     1321 agacgctggc cgcggtgttc gcgagccggg tggcgctcgc caagcgcacg ccggagcagg
     1381 cccaggccga cgcgcttctg gagcaccagc gcaccgaggc gtgggtgaag tcgccctcga
     1441 ccaaggaagc cagcttcctg tggttctgcg acgagttcga cctcgacgcg ggggcggtgc
     1501 gcaaagccat acgggagcgc cgggggtagc gtgcccgagc ctcgccccta ccagatcgaa
     1561 ggccgcgact tcctcgccgc gcgccggcat gcgctgctcg ccgacgagat gcgcgtgggc
     1621 aagacgcccc aggcgatcct ggccgcgcac aaggcgggcg cgcgctcgat gctggtcgtg
     1681 tgcccggcgg tggcggtcct gcagtgggag cgcgagatcg agcggtggtg gccttctggc
     1741 ccgctaccgc gcaccagggt ctggtcctac gataaggcgc gcgcgaactg ggtgatgggt
     1801 acgcgcgggg cggtggacgt cttcatccct gacgagtgcc acttcgctgg caaccccgag
     1861 gccgcacgca cccgcatggt gtacgccaag cacagcttcg cgtggtccgc cggggcgacg
     1921 tggccgctat caggcacgcc ggcgccgaag agcgccgcgt ctctgtggcc tatgctgcgc
     1981 gcattcggca tcgtcgacat gacctacgaa gacttcctga cgcggtactg cacgctgcgg
     2041 ccgatgtcgt gggtgccgac ggggacgaaa gtcaagacgg ccccggagtt gaacgcgatg
     2101 ctggcgaaaa tcatgctgcg ccggacgcgc cgccaagtgc gcccggacat gccggacatc
     2161 gggttcaact tcctcgaggt gaagcctgag acgagcgcca gctacgtcgt accgccgggt
     2221 ttgtcggacg ctgcgctgct tgagtggatg gcgctcaacg agcacgcaaa ccgcgagggc
     2281 cgcgtcgaag tggcgctcgc caagggcgga ccgctcgctg aagagatcgc cttcgccatc
     2341 aacaacgggc tgctgaagca gaccgtggtg ttcgggtggc acattgcgcc gctcgagtgg
     2401 ctcgtcggca cgctgaacgc gcgcggcatt cgcgccgccg ccatcacggg gaagacgccg
     2461 cgcaccgagc gcgagcgcat acagaacgag ttccgtgccg ggcgcctcga cgtggtgatg
     2521 gcgaacatcc tggcagcggg caccgccatc gacctgtcgg ccgcccggca cgggtacttc
     2581 ctcgagatgg actgggtgcc tggcaacaac gtgcaggcgg cgaaccggct ggtcagtctg
     2641 gatcgaagcg aggcggtgac gttcgacgtg gtgacgtggc ccggctcggc agatgatcgt
     2701 gtgcaaaacg ttttgttgac aagagtcgcg cagctagcta aactgtattg acactggagg
     2761 aaacgatggt caagatcacg atggagttcc cgaccctgga cgcggcgatc gtcgcgctgg
     2821 gcaaactctc gagcgcggcc gacacgacga tcgtcgacgg caagccgcgc cgcgggcgca
     2881 gcgacaaggg tctgccgcgc aagtcgcggt cggcgccgac ggaatcgaat gccaaagggg
     2941 agggggcggg cgcacagtcg gaaggggccg ccgaggccac cgtcgcgagc gccgcgacgc
     3001 cggctgccgc ccccgccccg gcggcatctc aggtcgctgc ggcggcaggc acgaaggacg
     3061 gcggtaccag caccaacgcc gagcctgccg ccaaggcacc ggacgtggcg ggtgccaaac
     3121 cggcaaccgc cgaggacgcg cagaaggcga tcgagaagct gttcgacgcc ccgcctccgg
     3181 tcggcggcct cgagcaagcg cgcatcgtca tgagccggtt cggcgtgaag cgcgtgcgcg
     3241 acctgcagga agaccagcgc gccgagttca tcgcgctcgc ggagaaggtg ctcgccggag
     3301 gtgcggcatg aaccccggct tctacagcct gacgtgcatc ctgtctgcgg ctctcgccgc
     3361 cgcgaaagat ccggtcgacc gggcggccct gttcgtcgcc atcaacggcg tcgccaacga
     3421 ggaaaatcaa tgagtggcgt ccacgcccgt ctgtccgcca gcgccgcgca ccgctggctg
     3481 aagtgcccgc cctcggtgaa cgccaagggc ggcggcagtt cgattcacgc agccaccggc
     3541 accttcgcgc acgacatcgc ggcgaggtgt ctgaacgacc cgtccatctc cgcgtccgac
     3601 tttttcctga agaaagagaa ggtcgatggt ttcgaggtcg agtgtgacct ggagatggtc
     3661 gacgcggtca acctttacct ggacgagatc gcggccgacc tgcagcccgg cgacctgacc
     3721 tgggtggaga tgccgcttct gcaggcgctc tcgcgcatcg acccggatct cggcggcacc
     3781 gccgactacg tgcgctaccg cccggccacc cggcagctgc tggtgatgga cttcaagtac
     3841 ggcagcggcg tgtacgtcga ggccgacgac aacgaccagt tgaagctgta cgccgtgggc
     3901 gccatgctcg agtgcggccg gtcggtcaac gaggtcacgg tcggcatcgt gcagccgcgc
     3961 ttcgagggcg cgaagcccgt gcgcacctcg gtgttcaagg gccacgaact gctcgagttt
     4021 gtcgccgacg tgcaggaggc cgccaacaag tcgcgcgacc cgaacgcgcc gctctcggcg
     4081 gggaaccact gcaagttctg cccgctcgcg cgcacctgtc cggaactgga gcggcgccac
     4141 cacgaactcg tggccgcgga tttcggcgtc gtcggcgatg cgacggtagc gccgacggcc
     4201 gctgcgcccg gcgcgctcgt ggactacagg gcgctggcgg tggcgctcga ctcggtgccg
     4261 ctcgtgaaag agcgcatcaa ggcgatcgaa gagttcgcct accaggaggc actgaaaggg
     4321 gcggagattc ccggctacaa gctggtggac aagcgcccgg tgcgccgctg gaagtccgag
     4381 ggcgaggtga ttctgtgggc cgaggcccag gcgatcgacc cgtacgcccc gcgctccgtc
     4441 gtcagtcctg cgcaactcga gaagaaactc gctgagtccg ccccgaaggg gaaaaagaaa
     4501 gaggccggca aggtactcga gccgttcgtc gagcgcgtgt ccagcggcac ggcgctcgtc
     4561 gcggcgacgg acgcgcgccc gccggcgaag cgggtgacgg cagaagattt cgcggtgatc
     4621 gccgaggatc gcggataaga gtgaagcagt ccccctcggt actacaggag aagcaaacga
     4681 tggcaaaagt gacactgaaa gatgtgcggc tcacgttccc ccaactcttc gagccgaagc
     4741 aggtcaacgg ccagggcgac ccgaagttct cgggcgcgtt cctgttcgcc aagaaccacc
     4801 cgcaggtcgc cgacatcaag gcggccatcg tcgaagccgc cacggcgaag tggggtgcga
     4861 agcacgccga ggtgctgaag gcgctctacg cgggcgacaa gctgtgcctg cacgacggcg
     4921 atgcgaagag cgactacgac ggctacgccg ggaacctctt catcaacgcc agcaacaaga
     4981 tccgcccgct ggtcatcgac gggcagcgcg ccccgctggt ggcggccgat ggtaagccgt
     5041 act
//
LOCUS       DBNHKE010000247         4753 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1266919, whole genome shotgun sequence.
ACCESSION   DBNHKE010000247 DBNHKE010000000
VERSION     DBNHKE010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1266919"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1650
                     /locus_tag="AC16O9_16775"
     CDS             136..1650
                     /locus_tag="AC16O9_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HIR6518310.1"
                     /translation="MTANELLPLAVLASSLVPGLVIFTLPEAWVGTRSALNLFGALAK
                     LALVGVLLAGVNEGQDYAVRYEALPGLDIAFKADGLGLLFVTLSALLWLLTTLYAIGY
                     LERSPRRSRFFGFFSICVTATMGIAMAGNLFTFFVFYELLTLSTFPLVVHRGTEQAMK
                     AGTLYLAYTLAGGTVLLTGIVWLHHLLGHTEFAHGGIVAALGPQAAGQLKVIFVLLIA
                     GLAVKAALVPLHGWLPRAMVAPAPVSALLHAVAVVKAGAFGIIRIVYEIYGIELAQSL
                     ELLTPLAVVAAATILWGSLRALFQDDLKRRLAFSTVSQVAYIVLGVTLFGPVGTVGGL
                     VHLVHQGIMKVTLFFCAGNFAETLGIHKVSETDGAGRRMPLSAAAFSIAALGMMGAPL
                     TAGAISKAWLIDGAHAAGMDWAAWVLWTSSLLNAAYFLPILGRLWLRRAPAAWPEERI
                     ARRGWRETAWLLLLPPLATAAATIAAGIFSQVELSPLDWARLIAEREYPPPAAE"
     gene            complement(1670..3097)
                     /locus_tag="AC16O9_16780"
     CDS             complement(1670..3097)
                     /locus_tag="AC16O9_16780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HIR6518311.1"
                     /translation="MTHAAGIALLLALYVALALLPLALAAAQGLPRRPWLDELSSGLA
                     MAGFSILLLEFALSGRSRRLSERIGMDLTMRFHQFMALAALAFVMVHPFLYIPAFGER
                     PAYGAASASRVLLTPAAAVTGMLAWILLAMLIAFAWFRTQLRWSYELWRLSHGLGALA
                     IAALGAHHTLDIGRYAQHAWLHGFWLAAWAAALAALAVIYLVKPALQSRRRWRVANVA
                     PEAERLWRVVLEPEGESDFRFAAGQFVWVKLRRALGRITEHPFSIASAPGQLPRLQFL
                     VKEAGDFTRDIGRVAPGTAAYVDGPYGNFTLAGREGAGIMLIGGGAGIAPVLSLARDL
                     AATHDPRPLKIVYADRSAAQLAARAELEVMARAPGRELHFVVAEPPADWRGLTGWLDL
                     ANLRKCVPRSGAADWLYFVCGPPGMIDAVEMGLAELGVPLAQITSERFVYDTGIATPR
                     ERLTRAVIAAVVAAQLAAVIAFVLR"
     gene            complement(3168..3908)
                     /gene="fnr"
                     /locus_tag="AC16O9_16785"
     CDS             complement(3168..3908)
                     /gene="fnr"
                     /locus_tag="AC16O9_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HIR6518312.1"
                     /translation="MSNVTTIQPAAANLKFEVTCSSCNLRELCLPAGLSAEDLARVEN
                     VVYARRRLKRGEALYQAGGDFSAIYAIRSGFFKTSLLDNDGREQVTGFFMGGELLGMD
                     GIGGSYHGTASALEDSEVCILPYALIEDVSRDIPTLQRQLHAVLAREIARDHGVMMLL
                     GSMRAEERLATFLLNLSKRFQRRGYSGTEFHLRMTRDEIGSYLGLKLETVSRLFSAFQ
                     KDGLIEVQQKHVRIRDSEGMEQVLASRR"
     gene            complement(3921..4286)
                     /locus_tag="AC16O9_16790"
     CDS             complement(3921..4286)
                     /locus_tag="AC16O9_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6518313.1"
                     /translation="MPTRNVYLVEDSPLVSERLLEMLESVPGVRVTGRAAGADEAIRE
                     ILAARPDVVVLDIRLAQGSGFDVLRTLHDRAPEIEVYMLSGFATEPYRHLAARLGARG
                     FFDKSTEFARVRDAVARPH"
     gene            complement(4355..>4753)
                     /locus_tag="AC16O9_16795"
     CDS             complement(4355..>4753)
                     /locus_tag="AC16O9_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6518314.1"
                     /translation="PTLRVRVLSMHGEQQYAARALKAGASGYLNKDSAAEQLVGALRK
                     VAGGGFHLSETAAAGLVAGTRGALHETLSDREIEVLRLLVEGLTPAQIGKRLHLSVKT
                     VSTHKTRAQDKLGAGSTAELVRYALEHKLV"
BASE COUNT          650 a         1677 c         1707 g          719 t
ORIGIN      
        1 ggcgccggcg gcgggcgcgc tgcggccggt gccgcgcacg ctggagtgga ccgcgtttgc
       61 gctcgccgcc gcgagcgtgc tgctcgggtt tcgcggcgtc gacctgctgg cgctgcttgc
      121 ggcgggaggc gcgccgtgac ggcgaacgag ctgctgccgc tcgcggtgct cgcctcgtcg
      181 ctcgtgccgg gcctcgtcat cttcaccctg ccggaggcct gggtcggcac gcgcagcgcg
      241 ctcaacctgt tcggcgcgct ggccaagctc gcgctggtcg gcgtgctgct cgccggcgtg
      301 aacgaaggcc aggactacgc cgtgcgctac gaggcgctcc ccggcctcga catcgcgttc
      361 aaggcggacg ggctcggatt gctgttcgtg acgctctcgg ctctgctgtg gctgctcacc
      421 acgctgtacg cgatcggcta cctcgagcgc tcgccgcgcc gcagccggtt cttcggcttc
      481 ttcagcatct gcgtcaccgc caccatgggc atcgcgatgg cggggaatct cttcacgttc
      541 ttcgtcttct acgagctgct gacgctgtcg actttcccgc tggtcgtgca ccgcggtacc
      601 gagcaggcga tgaaggcggg cacgctctac ctcgcctata ccctggcggg gggcacggtg
      661 ctgctcaccg ggatcgtctg gctgcaccat ctgctcggcc acaccgagtt cgcgcacggc
      721 ggcatcgtgg ccgcgctcgg gccgcaggcc gccgggcagc tcaaggtcat cttcgtgctg
      781 ctgatcgcgg gcctcgccgt caaggccgcg ctggtgccgc tgcacggctg gctgccgcgc
      841 gcgatggtgg cgccggcgcc ggtctcggcg ctgctgcacg cggtggcggt ggtcaaggct
      901 ggcgccttcg gcatcatccg catcgtctac gagatctacg gcatcgagct cgcgcaatcg
      961 ctcgagctgc tcacgcccct tgccgtggtc gccgcggcca ccatcctgtg gggcagcctg
     1021 cgcgcgctgt tccaggacga tctcaagcgg cggctcgcct tctcgaccgt cagccaggtg
     1081 gcctacatcg tgctgggcgt gaccctgttc gggccggtcg gcacggtcgg cggcctggtg
     1141 cacctggtgc accagggcat catgaaggtc acgctctttt tctgcgcggg caatttcgcc
     1201 gagacgctcg gcatccacaa ggtgagcgag accgacggcg caggtcggcg catgccgctc
     1261 agcgcggctg cgttctcgat cgccgcgctc ggcatgatgg gtgcgccgtt gaccgccggc
     1321 gcgatcagca aggcctggct catcgacggc gcgcacgccg cgggcatgga ctgggcggcg
     1381 tgggtgctgt ggacctcgag cctgctgaac gcggcctatt tcctgccgat cctggggcgg
     1441 ctgtggctgc gccgcgcccc ggcggcgtgg cccgaggagc gcatcgcgcg gcgcggctgg
     1501 cgcgaaacgg cgtggctgct gctgctgccg ccgctcgcga ccgctgctgc gacgattgcc
     1561 gccggcatct tctcgcaggt cgagctcagc ccgctcgact gggcgcggct gatcgccgag
     1621 cgggagtacc cgccaccggc ggccgagtag cggtgcagca cggatttggc tatcgcagca
     1681 cgaacgcgat gaccgcggcg agctgcgctg ccaccaccgc cgcgatcacc gcgcgcgtca
     1741 gccgctcgcg cggcgtggcg atgccggtgt cgtagacgaa gcgctcggag gtgatctgcg
     1801 ccagcggcac gccgagctcg gccaggccca tctccaccgc gtcgatcatg ccgggtggcc
     1861 cgcaaacgaa gtacagccag tcggccgcgc cgctgcgcgg cacgcatttc ctcaagttcg
     1921 ccaggtcgag ccagccggtc agtccgcgcc agtccgcggg cggctcggcg acaacgaagt
     1981 gcagctcgcg cccgggggcg cgcgccatca cctccagctc ggcgcgcgcc gcgagctgcg
     2041 ccgccgagcg gtcggcgtag acgatcttga ggggccgcgg gtcgtgcgtg gcggccaggt
     2101 cgcgcgccag gctcagcacg ggcgcgatgc cggcgccgcc gccgatcagc atgatgccgg
     2161 cgccctctcg cccggcgagc gtgaagttgc cgtaggggcc gtcgacgtag gccgcggtgc
     2221 ccggcgcgac gcggccgatg tcgcgcgtga agtcgcctgc ctccttcacc aggaattgca
     2281 gccgcggcag ctgcccgggc gccgaggcga tggagaacgg atgctcggtg atgcggccga
     2341 gcgcacggcg cagcttcacc cacacgaact ggcctgcggc gaagcggaaa tcgctctcgc
     2401 cctcgggctc gagcaccacg cgccacaacc gctcggcctc cggcgcgacg ttcgccacgc
     2461 gccagcgccg gcgcgactgg agcgcgggct tgaccaggta gatcacggcg agcgccgcca
     2521 gcgccgccgc ccacgccgcg agccagaagc cgtgcagcca agcgtgctgc gcgtagcgcc
     2581 cgatgtcgag cgtgtggtgc gcgccgagcg ccgcgatggc gagcgcgccc aggccgtgcg
     2641 agagccgcca cagctcgtag ctccagcgca gctgcgtgcg gaaccaggcg aaggcgatca
     2701 gcatggcgag caggatccag gcgagcatgc cggtcactgc cgccgccggc gtgagcagca
     2761 cgcgcgaggc gctcgccgcg ccgtaggccg ggcgctcgcc gaacgccggg atgtagagga
     2821 acgggtggac catgacgaag gcgagcgcgg cgagcgccat gaactggtga aagcgcatcg
     2881 tcaggtccat gccgatgcgc tcagacagtc gccgcgagcg gcccgagagc gcgaattcga
     2941 gcagcaggat cgaaaagccc gccatggcga ggccgctgga gagctcgtcg agccacggcc
     3001 ggcgcggcag gccctgcgcc gcggccagcg caagcggaag cagggcgagc gcgacgtaca
     3061 acgcgaggag cagcgcgatg cctgcagcat gcgtcatccg ccgcatggta cctacagcgc
     3121 tgctcagccc gcgtggtgcg cgaggcgctc cggtaagtgc acgtgcctca gcgccgcgac
     3181 gccagcacct gctccatacc ctcgctgtcg cggatgcgca cgtgcttctg ctgcacctcg
     3241 atcagcccgt ccttctggaa cgcggagaac aggcgactca ccgtctccag cttcagtccg
     3301 aggtagctgc cgatctcgtc gcgcgtcatg cgcaggtgga actcggtgcc ggagtagccg
     3361 cggcgctgga agcgcttgga caggttgagc aggaacgtcg ccaggcgctc ctcggcgcgc
     3421 atcgagccta ggagcatcat cacgccgtgg tcgcgcgcga tttcgcgcgc cagcaccgcg
     3481 tgcagctggc gctgcagcgt cgggatgtcg cgcgagacgt cctcgatcag cgcgtagggc
     3541 aggatgcaga cctcgctgtc ctcgagcgcg ctggccgtgc cgtggtagct gccgccgatg
     3601 ccgtccatgc cgaggagctc gccgcccatg aaaaagcccg tcacctgctc gcggccgtcg
     3661 ttgtcgagga ggctggtctt gaaaaagccg ctgcggatgg cgtagatggc gctgaagtcg
     3721 ccgcccgcct ggtacagggc ttcgccgcgc ttgaggcggc ggcgcgcgta gaccacgttc
     3781 tcgacgcgcg ccaggtcttc ggcgctcagc ccggccggca ggcagagctc gcgcaggttg
     3841 cagctcgagc aggtgacctc gaacttgagg ttcgcggcgg ccggctggat cgtggtgacg
     3901 ttggacatga agtgctcctc ttagtgcggg cgcgcaacgg cgtcgcgcac acgggcgaac
     3961 tcggtggact tgtcgaagaa gccgcgcgct ccgaggcgcg cggcgaggtg ccggtacggc
     4021 tcggtggcaa agccggacag catgtagacc tcgatctctg gcgcgcggtc gtgcagcgtg
     4081 cgcagcacat cgaaaccgct gccctgcgca agccggatgt cgagcacgac gacgtcgggg
     4141 cgtgcggcga gaatctcgcg gatcgcctcg tcggcgcccg ccgcgcggcc ggtgacgcgc
     4201 acgccgggga cgctctcgag catctcgagc aaccgctcgc tcaccaaagg cgagtcctcg
     4261 acgaggtaga cgttgcgcgt tggcatgccg ccagtgtggt gccgcgccgc cgcgccggcc
     4321 atcggcggcg gctgattctc ggttcggaaa tttcctagac cagcttgtgc tccaaggcgt
     4381 agcgcacgag ctcggcggtg ctgccggcgc cgagcttgtc ctgcgcgcgc gtcttgtgcg
     4441 tgctcaccgt cttcaccgac aggtgcaagc gcttgccgat ctgggccggg gtgagtccct
     4501 ccaccagcag ccgcagcacc tcgatctcgc ggtcggacag cgtctcgtgc agcgccccgc
     4561 gcgttcccgc gaccagtccg gcggcggcgg tttcactgag gtggaagccg cccccggcca
     4621 ccttgcgcag ggcgcccacc agctgttccg cggcgctgtc cttgttcagg tatcccgaag
     4681 cgcccgcctt cagggcgcgc gccgcatact gctgctcgcc gtgcatcgac agcacccgca
     4741 cccggagcgt cgg
//
LOCUS       DBNHKE010000248        11492 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_262321, whole genome shotgun sequence.
ACCESSION   DBNHKE010000248 DBNHKE010000000
VERSION     DBNHKE010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_262321"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC16O9_16800"
     CDS             complement(<1..457)
                     /locus_tag="AC16O9_16800"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6518315.1"
                     /translation="MIFHRALLREFAQLATAVFLSLFLIAITTRLVRLLGQAAGGKIP
                     TDAVVAFLGFFALNALPLLLSLTLFVTVLLTVTRSYRDSEMVIWFSAGLSLAAWVRPV
                     LAFALPLVALIATLSMVIAPWAVRMSEQYRSRVDARDDVARVDPGVFRES"
     gene            494..1999
                     /locus_tag="AC16O9_16805"
     CDS             494..1999
                     /locus_tag="AC16O9_16805"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR6518316.1"
                     /translation="MEFSIRALAPQGAKTGCLVLGVYQATGAGAPVLTRAAAAADRAA
                     RGRLATVLAHGDLSAKPGSTLLLHNVPGLAAERVLLVGLGEQKEFAETAFRKAVHGAA
                     VALRELGARDAVLYFAELKVGRRAMAWSVRQAALALRDAFYRFDQLKTQKKPPAPALA
                     EVTLAIHGEAVTEQARNALREAAATAEGVALARTLGNLPPNICTPSYLADEAKKLARQ
                     FKLGLEVLERRDMQRLGMGALLAVTRGSHQPPKLIVLRYMGGAKKAAPLVLVGKGITF
                     DTGGISLKPAGEMDEMKYDMSGAGSVLGAIRALAGMKAPVNVIGVVPTCENMPGGQAT
                     RPGDVVKTLSGQTVEILNTDAEGRLILCDALTYAERFKPDAVIDIATLTGACVIALGH
                     VATGLFANDDKLAEKLRAAGEDAWDRVWPMPLWEDYQEQLRSNFADMANIGGRPAGSV
                     TAACYLARFTKKLRWAHLDIAGTAWKSGRDKGSTGRPVPLLVRFALANAGR"
     gene            1996..2430
                     /locus_tag="AC16O9_16810"
     CDS             1996..2430
                     /locus_tag="AC16O9_16810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR6518317.1"
                     /translation="MTSVDFYFNAADKLQVACRLAGKALAQGKRLLIYAPQPDTAQSI
                     DRLLWTWPATGFVPHCRDGDALAAETPVLIAADVGSAAECDILLNLGPDTPPAFERYE
                     RLLEVVARDDGDRQAARERYRFYRERGYRVASHDLAGASRSE"
     gene            2423..2779
                     /locus_tag="AC16O9_16815"
     CDS             2423..2779
                     /locus_tag="AC16O9_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518318.1"
                     /translation="MSDPNPLVQRVDALLRRHQEASQGGGDDLPVLTEIVDPAKPAGP
                     AALDAAAVEALSRELERAVLARLENELQRTLAALGDGLRAALREAVAGAVARELQARK
                     LDAGALAAKRSTSTGS"
     gene            2782..5508
                     /locus_tag="AC16O9_16820"
     CDS             2782..5508
                     /locus_tag="AC16O9_16820"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6518319.1"
                     /translation="MSLDKSFDPHAVEARWYGRWEAAGWFAHRADARPAYCIQLPPPN
                     VTGTLHMGHAFQQTLMDALIRYHRMRGANTNWVVGTDHAGIATQIVVERQLQDEGKSR
                     HELGREKFVDRVWQWKQRSGASIMGQMRRLGASGNWAYADTEGQKAGYFTLDAKMSRA
                     VVEVFVRLYEEGLIYRGKRLVNWDPVLGTAVSDLEVDSEEEDGKIWEIRYPGEGFSVT
                     VATTRPETMLGDVAVAVNPKDERYAALVGKTVRLPLTGRTIPVIADDYVDPAFGTGCV
                     KITPAHDFNDFAVGQRHGLAPIEVLTLEAKVKGTPYAGLDRFEARKRVLADLKQQGLL
                     VSEKPYRLRVPRSGRTNEIVEPMLTDQWFVKMEGLARSGLEAVASGEVKFFPEHWRST
                     YDHWLENIQDWCISRQLWWGHQIPAWYDESGNITVARSEDEARAKAGGAKLRRDEDVL
                     DTWFSSALVPFSSLGWPEKTKDLDVFLPSSVLITGFDIIFFWVARRIMMTRHFTGRVP
                     FRHVYINAIVRDAEGEKMSKSKGNTLDPLDLIDGITLPELLAKSTLGLLRADHKAKIE
                     KYVRAHYPQGIPAFGADALRFTFAAQATFARTLNFDLGRCEGYRNFCNKLWNATRFVL
                     MNTEGRDCGQDDSAAVELSAWDRWIVSTLQRAEAEVEKGFDEYRFDNAAGAIYRFVWD
                     EYCDWYVEVAKEQLAKGNQAQQRGTRRTLVRVLEAALRLAHPIIPFVTEELWQRVAPL
                     AGKTGETIMLAPYPRSQPEKIDEAAEREIALAKEVVNAGRNLRSEAKIPPKERIPFYV
                     TGTLSASAAAGFVTLARVSDLRSVADLPASDSPVAVAGPHKIMPHIEVDPEAERERIA
                     KEVNRLEAEIAKARAKLANASFVERAPAHVVQQERDRLQAFQATLDKLAAKT"
     gene            5505..6767
                     /locus_tag="AC16O9_16825"
     CDS             5505..6767
                     /locus_tag="AC16O9_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518320.1"
                     /translation="MTLPHGLRAFRHRSFRLFFLGQGVSQFGTWLQLIATSWLIYRLT
                     GSALYLGLASFALQIPFLVLAPFAGVWVDRLEKRTVLRITNSIALTQSLVMLALVATG
                     LIQPWHLIAGNLVLGVVNAFDSPARQSFLIELVEGREDLPNAIAFQSMLMNGARFLGP
                     MIGGAVIALFGEVWGFALNSASYLAILAALAWLTVHARAPEGAPERWLSGLAAGFRWA
                     YGFGPSRNLLLLLAAVSIGVQPVQALAPYFARDVFGGDSQTLGWLIGAGGFGAVSGML
                     YLALRSTVRGLLAVIGGAGAAAGAAVIAFTWTSQLWLALPLVYLSGMGIMLTAGSTNT
                     ILQTIVEDRLRARVAAIYIMSFLGMSPLGALLAGWAAQYLGPPAALALGGGLALCASL
                     AYLARLPAMRRELRPLYAQLGITPRPVQ"
     gene            complement(6761..7390)
                     /locus_tag="AC16O9_16830"
     CDS             complement(6761..7390)
                     /locus_tag="AC16O9_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR6518321.1"
                     /translation="MSSRRRFLQGAAALLLVREALAAGTLEKGVYRVSGAARVNDRTA
                     VRGMEVKPGDAVTTAAGGEIIFVVNRDAFLLRENSRLEVGAVANVFRIVAGALLSVYQ
                     PGVRKTLHAQNATIGIRGTAVYVESGADKTYVCTCYGEAELTPLADPAAREIVRTRHH
                     EQPRYILPKGAPQMMMKAPVVNHTDAELAMLESLVGRGVPFDPTDYNRY"
     gene            complement(7387..7827)
                     /locus_tag="AC16O9_16835"
     CDS             complement(7387..7827)
                     /locus_tag="AC16O9_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518322.1"
                     /translation="MRMHLAADGETAALCAVLAAAQPAAADPLLVWLRSSGACAFALL
                     APLRLAPGKAWRWLAWGAMPAVATCRTFGLPAYLEGGAIWLHGRRAAGLRATHKGQCA
                     VIEGAFPAPLAAEPRLESAFRERIEAQHGWRFDTAWPSGGEARR"
     gene            7902..8891
                     /locus_tag="AC16O9_16840"
     CDS             7902..8891
                     /locus_tag="AC16O9_16840"
                     /inference="COORDINATES: protein motif:HMM:NF042524.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR6518323.1"
                     /translation="MQTEHTATANGNPAAGSRWLVTVTQFPTEDPAGRMRMLRTLESL
                     GAAVMREGVYLLPDTADNRYALERLAQYVAQCAGIASVLRVEAADAAQQAQLRRYFDR
                     SARYDELTKNVLSLRVGFGVSEASAIARVLSKQRRNFEAIAALDFFPNEARARTEQAL
                     AQAEAEVRALLSQALPGAAAPGAEPLQRRTWATRRPLWADRLACAWLIRRFIDSEAKI
                     VCLDKSAACPEDALGFGFEGARFASSEARTTFEQMLSHFGLAGHAALGRIGGIVHFLE
                     VRGTPVADAAGVQTLLQAAVRRGANDDELIREAEKAFDLLYEAYSSQRETTRA"
     gene            complement(8892..10334)
                     /locus_tag="AC16O9_16845"
     CDS             complement(8892..10334)
                     /locus_tag="AC16O9_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6518324.1"
                     /translation="MRLPTLCLAGLMVFALRAPAQPLPELGDAAGAAIAPQVERRIGE
                     SIVREIRYRDPAYVDDAEVTAYLDSLGRRLLSAIPDARHDFEFFAVRDPAINAFALPG
                     GFVGVHTGLLVAAESESELASVLAHEIAHVTQRHIARLIGAQQQMQLPSMVALAAAIL
                     LGGSRPDLAVGAAAAAQGGVIQQQLSYTRDFEREADRIGARTLQEAGFDVRAMPSFFE
                     KMQRYGRLGDDGTVPSYLRTHPMTTERIADAQGRAASAKYRQHLDSLDFHLVRAKLRA
                     EGGEPLDAVAAFDAAVRERRYANEAAARYGLVSARLRARQFAEVRADLAALRATGAAS
                     PMIETLAARVQQASGDAAGALATLGTALQRYPGRPPLIHAYALALYQAGRHAEALEAL
                     DAPVRRHPEDARLRALQAQTYAALGKRLLQHQAQAELYLLRGSVPAAIEQLQLARAAG
                     DGNFYELSAVEARLKELRARHAQEQRDAKR"
     gene            10377..10877
                     /gene="moaC"
                     /locus_tag="AC16O9_16850"
     CDS             10377..10877
                     /gene="moaC"
                     /locus_tag="AC16O9_16850"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR6518325.1"
                     /translation="MAGKPRARRALTHFDRGGRAHMVNVGAKARTHRVAVASGRILMR
                     ATTLRLLADGRAKKGDVLGVARIAALQAAKRTPELIPLAHPLAITRAAVDFELQPRQG
                     AVAIVATVECRGPTGVEMEALTAAAVGLLTVYDMLKAVDRGMVLTDLRLEAKRGGRSG
                     AWRRTP"
     gene            10874..>11492
                     /locus_tag="AC16O9_16855"
     CDS             10874..>11492
                     /locus_tag="AC16O9_16855"
                     /inference="COORDINATES: protein motif:HMM:NF052711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6518326.1"
                     /translation="MNLLRTGLVVAALAAAAGGYAWLKIGNGGGEHPQYRLARAERGP
                     LTAAVAASGTLNAVTTVQVGSQISGQVKEIFADFNTAVKAGQVIARIDPETYELRVNQ
                     ARADLDAAQSAVAVARSGLAAQMAELGRVKVTLADAQRDHERKKALVERSFISPSELD
                     KARTLLDAMREQLKAVEAQIRVGEAQVKSALAAVKQRESLLKQAQV"
BASE COUNT         1766 a         4039 c         4048 g         1639 t
ORIGIN      
        1 gcgactcgcg gaacacgccc gggtcgacgc gcgccacgtc gtcgcgagca tccacgcggc
       61 tgcggtactg ctcgctcatg cgcacggccc agggcgcgat caccatcgac agggttgcga
      121 tcagcgccac cagcggcagg gcgaacgcga gcaccgggcg tacccaggcg gcgagcgaca
      181 ggccggcgct gaaccagatc accatctcgg agtcgcggta gctgcgcgtc accgtgagca
      241 gcactgtgac gaacagcgtc agcgacagca gcaacggaag cgcattgagc gcgaagaagc
      301 cgaggaaggc gaccacggcg tcggtcggaa tcttgccgcc cgccgcctgt ccgagcaggc
      361 gcaccaggcg cgtcgtgatg gcgatcagga acagcgacag gaacaccgcg gtcgccagtt
      421 gcgcgaactc acgcagcagc gcgcggtgaa agatcatgcg atacttaaag cactcattac
      481 acgggagatt cgggtggaat ttagcatacg tgcgctggcg ccccagggcg cgaagaccgg
      541 ctgtctcgtg ctcggcgtct atcaggctac cggcgccggc gcacctgtgc tcacgcgcgc
      601 cgcggctgcg gctgaccgcg ctgcgcgcgg ccggctcgcc acggtgctgg cgcacggcga
      661 cctgagcgcc aagcctggct cgacgttgct gctgcacaac gtgcccggcc tcgctgcgga
      721 gcgcgtgctc cttgtcgggc tgggggaaca gaaggaattc gcggaaaccg cctttcgcaa
      781 ggccgtgcat ggcgctgcgg ttgcgctgcg cgagctcggt gcgcgcgacg ccgtgctcta
      841 cttcgccgag ctgaaggtcg gtcgtcgtgc catggcctgg agcgtgcggc aggcggccct
      901 ggcgttgcgc gatgcctttt accgcttcga ccagctgaag acgcagaaga agccgcccgc
      961 acccgcgctg gccgaggtga cgctcgcgat ccacggtgaa gcggtgaccg agcaggcgcg
     1021 caatgcgttg cgcgaggcgg cggcgacggc tgaaggcgtc gcgctcgcgc gcacgttggg
     1081 gaacctgccg cccaacatct gcacgccctc ctacctggcg gacgaggcga agaagctcgc
     1141 gcgccagttc aagctcggcc tcgaggtgct ggagcgccgc gacatgcagc gcctgggcat
     1201 gggggcgctg ctggcggtaa cgcgcggctc gcaccagccg cccaagctga tcgtcctgcg
     1261 ctacatgggc ggcgcgaaga aggctgcgcc gctggtcctg gtcggcaagg gcatcacctt
     1321 cgacaccggc ggaatttcgc tgaagcccgc cggcgagatg gacgagatga agtacgacat
     1381 gtcgggcgcc ggcagcgtgc tgggcgcaat acgcgcgctg gccgggatga aggcgccggt
     1441 gaacgtcatc ggcgtggtgc cgacctgcga gaacatgccc ggcggccagg cgacgcgccc
     1501 gggcgatgtg gtgaagaccc tgtcggggca gaccgtggag atcctgaaca ccgacgccga
     1561 gggtcgcctc atcctgtgcg acgcgctcac ctatgcggag cgcttcaaac ccgacgcggt
     1621 aatcgacatc gcgacgctca ccggcgcctg cgtcatcgcg ctggggcacg tggccacggg
     1681 cctgttcgcc aacgacgaca agctcgccga gaagctgcgc gcggccggcg aggacgcctg
     1741 ggaccgcgtg tggccgatgc cgctgtggga ggactaccag gagcagctgc gctcgaactt
     1801 cgccgacatg gcgaatatcg gcgggcggcc cgcgggcagc gtcaccgcgg cgtgctatct
     1861 cgcccgcttc accaagaagc tgcgctgggc gcacctcgac atcgccggca cggcgtggaa
     1921 gagcggccgc gacaagggct ccaccggccg gccggtgccg ctgctggtgc gctttgcgct
     1981 cgcgaacgcg ggacgatgac cagcgtcgat ttctacttca acgccgcgga caagctgcag
     2041 gtggcctgcc gcctggccgg caaggcgctg gcgcaaggca agcggctgct gatctacgcc
     2101 ccgcagcccg ataccgcgca gagcatcgac cggctgctgt ggacttggcc ggccaccggc
     2161 ttcgtgccgc actgccgcga cggggacgcg cttgccgcgg agacgcccgt gctcatcgcc
     2221 gccgatgtcg gcagcgccgc cgagtgcgac atcctgctca acctcgggcc ggacacgccg
     2281 cccgcattcg agcgctacga gcgcctgctc gaggtggtgg cgcgcgacga cggcgaccgc
     2341 caggcggcgc gcgagcgcta ccgcttctac cgcgagcgcg gctaccgcgt cgccagccac
     2401 gatctcgccg gagcgagccg cagtgagtga tcccaatcct ctggtgcagc gcgtcgatgc
     2461 gctgctcagg cgccaccagg aggcgtctca gggcggcggc gacgacctgc cggtgctgac
     2521 cgagatcgtc gatccggcca agcccgcggg gccggccgcc ctcgatgcgg ccgccgtgga
     2581 ggcgctgtcg cgcgagctcg agcgtgcggt gctggcgcgc ctggagaacg agctgcagcg
     2641 caccctcgcc gcgcttggcg atgggctgcg cgctgcgctg cgcgaggcgg tggcgggtgc
     2701 cgtggcgcgc gagctgcagg cgcgcaagct cgatgccggc gccctcgccg ccaagcgctc
     2761 cacctccacg ggcagctgag gatgtcgctc gacaagagct tcgatccgca cgcggtcgag
     2821 gcccgctggt atgggcgctg ggaagctgcg ggctggttcg cgcaccgcgc tgacgcgcgg
     2881 cccgcctact gcatccagct gccgccgccg aacgtcaccg ggacgctgca catggggcat
     2941 gcgttccagc agacgctgat ggatgcgctg atccgctacc accgcatgcg cggcgcgaac
     3001 accaactggg tggtcggcac cgaccacgcg gggatcgcca cgcagatcgt ggtcgagcgc
     3061 cagctgcagg acgaaggcaa atcgcgccac gagctggggc gcgagaagtt cgtcgatcgc
     3121 gtctggcagt ggaagcagcg ctccggcgcc tcgatcatgg gccagatgcg ccgcctgggc
     3181 gcctcgggca actgggccta cgccgacacc gaaggccaga aggccggcta cttcaccctg
     3241 gacgcgaaga tgtcgcgcgc cgtggtcgag gtgttcgtgc ggctgtacga ggaaggcctg
     3301 atctaccgcg gcaagcggct cgtcaactgg gatcccgtgc tcggcaccgc ggtgtccgac
     3361 ctcgaagtgg acagcgagga ggaagacggc aagatctggg agatccgcta cccgggcgaa
     3421 ggcttcagcg tgacggtggc gaccacccgg ccggagacca tgctcggcga cgtcgcggtc
     3481 gcggtgaacc ctaaggacga gcgctacgcc gcgctcgtcg gcaagacggt gcggctgccg
     3541 ctcaccggcc gcacgatccc ggtcatcgcc gacgactacg tcgaccccgc gttcggcacc
     3601 ggctgcgtga agatcacgcc ggcgcacgac ttcaacgact tcgccgtcgg ccagcgccac
     3661 gggctcgcgc cgatcgaggt cctgaccctc gaggcgaagg tgaagggcac gccgtacgcg
     3721 ggactcgacc ggttcgaagc gcgcaagcgc gtgctcgccg acctgaagca gcaggggctg
     3781 ctggtttcgg agaagcccta caggctgcgc gtgccgcgct ccgggcgcac caacgagatc
     3841 gtcgagccga tgctcacgga ccagtggttc gtgaagatgg aggggctggc gcggtccggc
     3901 ctcgaggcgg tggcgagcgg cgaggtgaag ttctttcccg agcactggcg cagcacctac
     3961 gaccactggc tggagaacat ccaggactgg tgcatctcgc gccagctgtg gtggggccac
     4021 cagatcccgg cctggtacga cgagtccggc aacatcaccg tggcgcgcag cgaggacgag
     4081 gcgcgcgcca aggcgggcgg cgccaagctg cgtcgcgacg aagacgtgct cgacacctgg
     4141 ttctcctccg ccctggtgcc gttctcctcg ctcggctggc cggagaagac gaaggacctg
     4201 gacgtgttcc tgccctcctc cgtactgatc acgggcttcg acatcatctt cttctgggtc
     4261 gcgcgcagga tcatgatgac gcgccacttc accggccggg tgccgttccg ccacgtctac
     4321 atcaacgcca tcgtgcgcga cgccgagggc gagaagatgt ccaaatcaaa gggcaacacg
     4381 ctcgatccgc tcgacctcat cgacggcatc acgctgcccg agctgctcgc caagtcgacg
     4441 ctcgggctgc tgcgcgccga tcacaaggcg aagatcgaga aatacgtgcg cgcgcactat
     4501 ccgcagggga tccccgcctt cggcgccgac gcgctgcgct tcaccttcgc ggcgcaggcc
     4561 acgtttgcgc gcacgctcaa tttcgacctc gggcgctgcg agggctacag gaacttctgc
     4621 aacaagttgt ggaacgcgac gcgcttcgtg ctgatgaaca ccgaagggcg tgattgcggg
     4681 caggacgaca gcgccgccgt cgagctgtcg gcctgggacc gctggatcgt gagcacgctg
     4741 cagcgcgccg aggccgaagt cgagaaaggc ttcgacgaat accgcttcga caacgccgcc
     4801 ggcgcgatct accgcttcgt gtgggacgag tactgcgact ggtacgtcga ggtggcgaaa
     4861 gagcagctgg ccaaaggcaa ccaggcgcag cagcgcggca cgcggcgcac gctggtgcgc
     4921 gtgctcgagg cggcgctgcg cctcgcacac ccgatcatcc ccttcgtgac cgaggagctg
     4981 tggcaaaggg tggcgccgct cgccggcaag accggcgaga ccatcatgct tgcgccgtat
     5041 ccgcgcagcc agccggagaa gatcgatgaa gcggcggagc gcgagatcgc gctggcgaaa
     5101 gaagtggtga acgccggccg caatctgcga agcgaagcca agatcccgcc gaaggaacgc
     5161 attccgttct atgtcaccgg gacgctgagc gcttccgccg cagcgggatt cgtgacgctg
     5221 gcacgggtat cggatctgag gagcgtcgcc gacttgccgg cgtcggactc cccggttgcc
     5281 gtcgccgggc cccacaagat catgccgcac atcgaggtcg atccagaagc cgagcgcgag
     5341 cgcatcgcca aggaagtgaa ccgcctcgag gccgagatcg ccaaggcccg cgccaagctc
     5401 gccaacgcgt ccttcgtcga gcgcgccccc gcgcacgtgg tacagcagga gcgcgaccgc
     5461 ctgcaggcgt tccaggccac gctggacaag ctcgccgcga agacgtgacg ctcccgcacg
     5521 ggctgcgcgc cttccggcac cgcagcttcc ggctgttctt cctcggccag ggggtatcgc
     5581 agttcggcac ctggctgcaa ctcatcgcca ccagctggct catctaccgc ctgaccggct
     5641 cggcgctcta tctcgggctc gccagcttcg cgctgcagat cccgttcctg gtgctggcgc
     5701 cattcgccgg ggtctgggtc gaccggctgg aaaagcgcac ggtgctgcgc atcaccaaca
     5761 gcatcgccct cacccagtcc ctggtcatgc tcgcgctcgt cgccacgggc ctgatccagc
     5821 cctggcacct gatcgccggc aacctggtgc tcggggtggt gaacgccttc gacagcccgg
     5881 cgcgccagtc cttcctcatc gagctggtcg agggccgcga agacctgccg aacgccatcg
     5941 ccttccagtc gatgctgatg aacggcgcgc gcttcctcgg tccgatgatc ggcggcgccg
     6001 tgatcgcctt gttcggcgag gtgtggggct tcgcgctgaa cagcgcctcc tacctcgcga
     6061 tcctggcggc gctggcgtgg ctcacggtgc acgcgcgggc gcccgagggc gcccccgagc
     6121 gctggctctc ggggctggcg gcgggctttc gctgggcgta cggctttggc ccttcgcgca
     6181 acctgctgct tctgcttgcc gcggtgagca tcggcgtgca gccggtgcag gcgctggcac
     6241 cctatttcgc gcgcgacgtg ttcggcggcg actcgcagac gctgggctgg ctgatcggcg
     6301 ccggaggctt cggcgcggtg tcgggcatgc tctatctcgc gctgcgctcg acggtgcgcg
     6361 ggctcctggc ggtgatcggc ggcgcgggcg cggccgccgg cgccgccgtg atcgcattca
     6421 cctggaccag ccagctgtgg cttgcgctgc cgctcgtgta cctctccggc atgggcatca
     6481 tgctcaccgc aggctcgacc aacacgatcc tgcagacgat cgtcgaagac cgcctgcgcg
     6541 cgcgcgtcgc cgccatttac atcatgtcct tcctcggcat gagccccctg ggagcgctgc
     6601 tggcgggctg ggcggcccag tacctcggtc cacccgcggc gctcgcgctg ggcggcggcc
     6661 tcgccctgtg cgcctcgctc gcatacctgg cacggctgcc cgcgatgcgg cgcgagttgc
     6721 ggccgctcta cgcgcagctc ggcatcaccc cgcgccccgt tcagtagcgg ttgtagtcgg
     6781 tcgggtcaaa cggcacgccg cgcccgacca gcgactcgag catcgcgagc tcggcgtcgg
     6841 tgtggttgac caccggcgcc ttcatcatca tctgcggcgc gcccttgggc aggatgtagc
     6901 gcggctgctc atgatggcgc gtgcgcacga tttcccgcgc cgcggggtcg gcgagcggcg
     6961 tgagctcggc ttcgccatag caggtgcaga cgtaggtctt gtcggcgccg ctctcgacgt
     7021 agaccgccgt gccgcggatg ccgatggtgg cgttctgcgc gtgcagcgtc ttgcgcacgc
     7081 cgggctgata gaccgagagc agcgccccgg cgacgatgcg aaagacgttc gccacagcgc
     7141 ccacttcaag gcgcgagttc tcgcgcagca ggaacgcgtc gcggttgacg acgaagatga
     7201 tctcgccgcc ggccgccgtg gtgacggcgt cgcccggctt cacctccatg ccgcgcacgg
     7261 cggtccggtc attgacgcgc gccgcgccgc tcacgcggta cacgcctttc tccaaggtcc
     7321 cggcggcaag cgcctcgcgc acgaggagca gcgccgcagc gccctgcagg aaacgccgac
     7381 ggctgctcac cggcgcgcct cgccgccgct cggccacgcg gtatcgaagc gccacccgtg
     7441 ctgcgcttcg atgcgctcgc gaaacgccga ctccagcctg ggctcggcgg cgagcggcgc
     7501 gggaaatgcg ccttcgatta ccgcgcattg ccccttgtgc gtggcgcgca ggcccgccgc
     7561 gcgacgccca tgcagccaga tcgccccacc ctccagatac gccggcaggc cgaacgtgcg
     7621 gcaggtggcg accgccggca tcgcgcccca cgccagccag cgccatgcct tgccgggggc
     7681 caggcgcagg ggcgccaaga gcgcaaacgc gcacgcgccc gaggagcgaa gccagacgag
     7741 aagcgggtcg gccgccgccg gctgggcagc cgcgagcacg gcgcacaacg ccgccgtctc
     7801 gccgtccgcg gcgagatgca tgcgcatcca tcgcgcagcc tgggccatga cttccgctta
     7861 tatatcaggc acgccgcgct atgcaataat cggcgcgcgc catgcagacg gaacacaccg
     7921 cgacggcgaa cggaaatccg gccgcgggca gccgctggct ggtcaccgtg acgcaatttc
     7981 ccaccgagga tcccgcgggc cgcatgcgca tgctgcgcac gctggagtcg ctcggcgccg
     8041 ccgtgatgcg cgaaggcgtc tatctgctcc ccgacacggc ggataaccgg tatgcactgg
     8101 agcggctggc gcagtacgtc gcacagtgcg caggcatcgc cagcgtgctg cgggtggagg
     8161 cggcggacgc ggcgcagcag gcacagctca ggcgctattt cgaccgctcc gcgcgctacg
     8221 acgagctgac caagaacgtg ctcagcctgc gcgtcggctt cggcgtgtcc gaggcctccg
     8281 cgatcgcgcg cgtactgagc aagcagcgcc gcaatttcga ggccatcgcg gcgctcgatt
     8341 tctttcccaa cgaggcgcgc gcacgcaccg agcaggcgct tgcgcaggcg gaggccgagg
     8401 tgcgcgcgtt gctgtcccag gcgctgccgg gcgctgcggc gcccggcgcc gagccgctcc
     8461 agcgccgcac ctgggcgacg cgccggccgc tgtgggccga ccgcctcgcc tgcgcctggc
     8521 tgatccggcg cttcatcgac tcggaggcga agatcgtttg tctcgacaag agcgccgcct
     8581 gccccgaaga cgcgctcggc ttcggcttcg agggcgcgcg ctttgccagc agcgaggcgc
     8641 gcaccacctt cgagcagatg ctgagccatt tcggccttgc cgggcacgcg gcgctcggcc
     8701 gcatcggcgg catcgtccac tttctggaag tgcgcggcac gccagtggcg gacgctgccg
     8761 gcgtgcaaac gctgctgcag gcggccgtgc ggcgcggcgc gaacgacgac gagctgatac
     8821 gggaggcaga aaaggccttt gacctgctct acgaggctta ctcgagccag cgcgaaacga
     8881 cgcgcgcctg atcagcgctt ggcgtcgcgt tgctcctgcg cgtgacgcgc gcgcaattcc
     8941 ttcaggcggg cctcgactgc cgagagctcg tagaagttgc cgtcgcctgc cgcacgcgcc
     9001 agctgcagct gctcgatcgc cgccggcacg ctgccgcgca gcaggtacag ctctgcttgc
     9061 gcctggtgct gaagcaggcg cttgccaagc gccgcgtagg tctgcgcctg cagcgcccgc
     9121 agacgcgcgt cctccggatg ccggcgcacc ggggcatcga gcgcttcgag cgcctccgcg
     9181 tggcgccccg cctgatacag cgccagcgcg taggcgtgga tgagcggcgg gcggcccggg
     9241 taacgctgca gcgcagtccc gagcgtggcc agcgctccgg cggcgtctcc cgacgcctgc
     9301 tgcacgcgtg ctgccagcgt ctcgatcatc gggctggccg cgcccgtggc gcgcagcgcc
     9361 gccaggtcgg cacgcacttc tgcgaactgg cgcgcgcgca ggcgcgcgct caccagcccg
     9421 tagcgggcgg ccgcctcgtt ggcgtagcgc cgctcgcgca ctgcagcgtc aaacgcggcc
     9481 accgcatcga gcggctcgcc gccttcggca cgcagcttcg cgcgcacgag gtggaaatcc
     9541 aggctgtcca gatgctgccg gtacttcgcg ctcgccgcgc gcccttgcgc atcggcgatg
     9601 cgctcggtgg tcatggggtg ggtgcgcagg tagctcggca ccgtgccgtc gtcgcccagc
     9661 cgcccgtagc gctgcatctt ctcgaagaag cttggcatgg cgcgcacgtc gaagcccgct
     9721 tcctgcagcg tgcgagcgcc gatccgatcc gcctcgcgct cgaagtcgcg cgtatagctg
     9781 agctgctgct ggatgacgcc gccctgggcc gccgccgccg cgccgaccgc gaggtccggc
     9841 cgcgagccgc cgagcaggat cgcggcagcg agcgccacca tgctcggcag ctgcatttgc
     9901 tgctgcgcgc cgatcagccg ggcgatgtgc cgctgcgtga cgtgcgcgat ctcgtgcgcc
     9961 agcaccgagg cgagctcgga ctcgctttcg gccgcgacca gcagcccggt gtgcacgccg
    10021 acgaaccctc cgggcagggc aaaagcgttg atggccggat cgcgcaccgc gaaaaactcg
    10081 aaatcgtgac gcgcgtcggg gatcgcggac agcaggcgcc ggccgaggct gtcgaggtag
    10141 gcggtcacct ccgcgtcgtc gacgtacgcg gggtcgcggt agcggatctc gcgcacgatg
    10201 ctctcgccaa tgcggcgctc cacctgcggc gcgatcgcgg cgccggccgc atcgccgagc
    10261 tcgggcagcg gctgcgccgg agcacgcagc gcgaaaacca tcaggccggc gaggcatagg
    10321 gtcgggaggc gcatgcgaat atgataccgt cgcgtccttc aagttccggt gcaggcatgg
    10381 caggaaaacc gagagcgcgg cgcgcgctga cccatttcga tcgcggcgga cgcgcgcaca
    10441 tggtcaacgt cggcgccaag gcgcgcaccc atcgcgtcgc cgtcgcctcc gggcgcatcc
    10501 tgatgcgcgc gacgacgttg cgcctgctcg ccgacggacg ggccaagaaa ggcgacgtgc
    10561 tcggcgtcgc gcgcatcgcc gcgctgcagg cggccaagcg cacgccggag ctgattccgc
    10621 ttgcccatcc gcttgccatc acgcgtgccg cagtggattt cgagctgcag ccgcggcaag
    10681 gcgccgtcgc gatcgtcgcg acggtcgaat gccgcggccc gaccggcgtg gagatggagg
    10741 cgctcaccgc ggccgcggtc ggcctgctca ccgtgtacga catgctcaag gccgtggacc
    10801 gtggcatggt gctcaccgac ctgcgcctgg aggccaagcg cggcgggcgc tccggcgcct
    10861 ggcggcgcac gccttgaatc tcctgcgcac cgggctggtg gtcgccgcgc ttgccgccgc
    10921 ggcgggcggc tacgcgtggc tgaagatcgg caacggcggc ggcgagcacc cgcagtaccg
    10981 cctggcgcgc gccgagcgcg gcccgctcac cgcagcggtg gcggcgagcg gcacgctgaa
    11041 tgccgtgacc acggtgcagg tcggctcgca gatctccggc caggtaaaag agatcttcgc
    11101 cgacttcaac acggcggtga aggcgggcca agtcattgcc cgcatcgacc ccgagaccta
    11161 cgagctgcgc gtcaaccagg cacgcgccga cctcgacgcc gctcagagcg cggtggcggt
    11221 ggcgcgctcc ggccttgccg cgcagatggc cgagcttggt cgcgtcaagg tcacgctcgc
    11281 cgacgcacag cgcgaccacg agcgcaagaa ggcgctagtg gagaggagct tcatctcgcc
    11341 ttcggagctc gacaaggcgc gcacccttct ggatgcgatg cgcgagcagc tgaaggcggt
    11401 cgaagcgcaa atccgcgtcg gcgaggcgca ggtgaagagc gcccttgccg cggtgaagca
    11461 acgcgagtca ctgctcaagc aggcgcaggt gg
//
LOCUS       DBNHKE010000249         5699 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_473216, whole genome shotgun sequence.
ACCESSION   DBNHKE010000249 DBNHKE010000000
VERSION     DBNHKE010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_473216"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16O9_16860"
     CDS             complement(<1..347)
                     /locus_tag="AC16O9_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518327.1"
                     /translation="MRRVLSLQLALVCVLTTQQGVIAAQPAPESLEPLPSEPSKRVFY
                     GEYYNPEQYARCLERRAKENRDPRIDGCYIYRERLEPLDPKRRDEFGEFYDPKRYHDC
                     RAGVEARDTQCEY"
     gene            513..932
                     /locus_tag="AC16O9_16865"
     CDS             513..932
                     /locus_tag="AC16O9_16865"
                     /inference="COORDINATES: protein motif:HMM:NF037100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HIR6518328.1"
                     /translation="MPNLTIQLDAALLKAAKVYAARHDTSISELVRRHLAELTRATNN
                     EIAAQLAAERSRIQALAKKRGLHNLRVFGSVARGEADDASDVDLLVDAHKGTSLIDLI
                     GFQRELAALLGRKVDVVTPAGLPPEVRSRALAEATPL"
     gene            929..1279
                     /locus_tag="AC16O9_16870"
     CDS             929..1279
                     /locus_tag="AC16O9_16870"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: protein motif:HMM:NF014043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR6518329.1"
                     /translation="MRTGDPAAARQARLQHIAEAIARIREDTGGGKKAFLASRVHQQA
                     VILNLLIIGEAVKHIDRESLSTQSGIPWTDITGMRDVIAHKYYHLDLDLVWDIITKNL
                     APLAAAVAALRRRG"
     gene            complement(1318..3522)
                     /locus_tag="AC16O9_16875"
     CDS             complement(1318..3522)
                     /locus_tag="AC16O9_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HIR6518330.1"
                     /translation="MATQPTAGGGAQGAVPKDDFVWVLGTLCALHRVPFDADLFLRQF
                     PPPYSVEKLLGALGALGFHAGQASTASIGARTTCPCIAFERAEAESGEPQLRVAIVAK
                     CDTGQAVVFKSSSRDPVSVPLARFDEAFVPVLVLLRRDLNEPADPDAKRARPAAFGFG
                     WFVPELLRHKPIWRDVLVASLAIQVAGLATPLFTQVVIDKVIVHHTQSTLWAVGVALG
                     MFMLFGAVMTWLRQYLVVHTGNRVDAVLGQSVFRHLFRLPMPYFEHRTTGVLVARLQG
                     VEQIRDFVSGAAVALLLDCPFLFVFLAVMFWYSWQLTLIALAVLLAIAILSLAVTPMF
                     RVKLDRQFLTGARNTAFVTEYVSGMATVKSLQLEPVLEERYGDQLAQYLAAGFETRQL
                     ANTYNVLANTLEQVMTLAILVMGALLVIDSSARAAAGAGAFTIGMLVAFQMFASRLSQ
                     PLLRLVGLWQQFQQARVAVRRLGDIMDMPAEPHALVPQRASSAQAGQIELKAVSFRYS
                     EQHPWLYRNLNLAIRPGQLTVLVGPSGCGKSTLAKLLLGFYLPTDGRILIDGQDIRTM
                     NANELRAGFGVVPQETTLFSGTVFDNLQMANPHASFEQIVEACRMAEIHDVIEALPQG
                     YQAPLGEHGVGLSGGQRQRIAIARALLKRPKVLIFDEATSNLDAPTAESIARTVNQLK
                     GMVTIIFIAHQLPRGLKVDEVVQFTPRAAAKAEAGPQAPTQVSVVEDRGGEG"
     gene            complement(3583..4209)
                     /locus_tag="AC16O9_16880"
     CDS             complement(3583..4209)
                     /locus_tag="AC16O9_16880"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6518331.1"
                     /translation="MHIRQRGHNRQACFFGGEDYALYLGLLGYFAREHACTVHAYCLM
                     TNHVHLLLTPQTDSGSALMMKSLAQCYTQHINRAHRRTGSLWDSRFRSCLVDSERYAL
                     ACYRYVELNPVSAGMVRHPREYPWSSYRANADGRADPLIRPHPAFPGAEPYRRLFDEA
                     LDQNLVDELRKATNGGFAAGAPRARKGRPRRNAGPQENEVCPHLVRAV"
     gene            4327..5412
                     /gene="hppD"
                     /locus_tag="AC16O9_16885"
     CDS             4327..5412
                     /gene="hppD"
                     /locus_tag="AC16O9_16885"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR6518332.1"
                     /translation="MAERWDNPMGTDGFEFIEYAAPDPQVMGALFERLGFRAVARHRH
                     KNVVLYRQGGVNFIVNAEPDSFAQRFARLHGPSICAIAFRVQDAKRAYERAVDLGAWG
                     YAGTAGPGELNIPAIKGIGDSLIYLIDRWRGKGGKKEGDIGNIGFYDVDFEPLSGAEL
                     NPAGYGLTVIDHLTHNVHKGRMKEWADFYERLFNFKEIRYFDIEGQVTGVKSKAMTSP
                     CGKIRIPINEEGTEKAGQIQEYLDLYHGEGIQHVALGSTDLFRTVDALRANGVKLLDT
                     VDTYYELIDKRIPGHGENIAALKKRKILVDGKKGALLLQIFSENQLGPIFFEFIQRKG
                     DEGFGEGNFKALFESIELDQMRRGVLK"
     gene            5479..>5699
                     /locus_tag="AC16O9_16890"
     CDS             5479..>5699
                     /locus_tag="AC16O9_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR6518333.1"
                     /translation="MRYQSGFGNTFSSEAEKGALPVGQNSPQKTPRGLYPEVLSGSAF
                     TAPRHENLSSWLYRLRPSAMHGPFKQLGQG"
BASE COUNT         1038 a         1943 c         1802 g          916 t
ORIGIN      
        1 aaatactcgc actgtgtgtc tcgcgcttct acgccagccc tgcaatcgtg atacctcttg
       61 gggtcgtaga actcgccgaa ttcgtcgcgt ctcttggggt ccagcggctc gaggcgctct
      121 ctgtagatgt agcaaccatc tatccgggga tcacgattct ctttggcgcg tcgttccagg
      181 cacctcgcgt actgctcggg gttgtagtac tcaccgtaga agactctctt gctgggctcg
      241 ctcggcagcg gttcgaggct ctcgggcgcc ggttgcgcag cgatcactcc ctgctgtgtg
      301 gtgagcacac agaccaacgc caactgcaag gaaagaactc ttctcacctt ttcgctcctc
      361 catcgtccac cacactcacc gccggcgcct gcgaccccgt ctccgccccg cgccaccgct
      421 tgagccccac gtcgctaggc gcgacaaaag ctggccgccc gcatcccaca tgttcccgtg
      481 gtactatcac gcctccacgt ggagcccccg ccatgcccaa cctgacgatc cagctcgacg
      541 ccgccctgct caaggccgcc aaggtgtatg ccgcccggca cgacacctcg atcagcgagc
      601 tcgtgcgacg gcacctcgcc gagctcaccc gcgcgacgaa caacgagatc gcggcgcagc
      661 tggcggccga gcgatcgcgc atccaggcgc tggcgaagaa gcgcggcctg cacaatctcc
      721 gggtgttcgg ctcggtcgcg cggggcgagg ccgacgacgc gagcgacgtg gacctgctcg
      781 tcgacgcgca caagggcacg agcctcatcg atctcatcgg cttccagcgc gagctcgcgg
      841 cgctcctcgg ccgcaaggtg gatgtcgtga cgccggccgg gctgccgccc gaggtccgtt
      901 cgcgcgctct cgcggaggcg acgccccttt gaggaccgga gaccccgccg ccgcgcggca
      961 ggcccgcctg cagcacatcg cggaggccat cgcccgcatc cgcgaggaca cggggggcgg
     1021 aaagaaggcc ttccttgcca gtcgcgtgca ccagcaggcc gtgatcctca acctgctgat
     1081 catcggcgaa gccgtgaagc acatcgacag ggagtcgctg tctacgcaat ccggcatccc
     1141 ctggacggac atcacgggca tgcgggacgt gattgcgcac aagtactatc acctggacct
     1201 ggatctggtc tgggacatca tcacgaagaa tctcgctccg ctggcggcag cggtcgccgc
     1261 cttgcggcgg cgagggtgaa gccgccgcgc gcgcgcctgc tcctgtccgc ggccgactca
     1321 cccttccccc ccgcgatcct ccaccacact cacctgcgtc ggcgcctgcg gccccgcctc
     1381 cgccttcgcc gccgcccgcg gcgtgaactg caccacctcg tccaccttca accccctcgg
     1441 caactggtgc gcgatgaaga tgatcgtcac catacccttc aactggttca ccgtccgcgc
     1501 aatgctctcc gccgtgggcg catcgagatt gctcgtcgcc tcgtcgaaga tcagcacctt
     1561 cggccgcttc agcaacgccc gcgcaatcgc gatgcgctgc cgctgcccgc cggacagccc
     1621 cacgccgtgc tcgccgagcg gcgcctggta gccctgcggc agcgcctcga tcacgtcgtg
     1681 gatctccgcc atgcggcacg cctcgacgat ctgttcgaag ctcgcatggg gattcgccat
     1741 ctgcaggttg tcgaacacgg ttcctgaaaa caacgttgtc tcctgcggca ccacgccgaa
     1801 cccggcgcgc aattcgtttg cgttcatggt gcggatgtcc tgcccgtcga tcaggatgcg
     1861 cccatccgta ggcaggtaga acccgagcag cagcttcgcc agcgtgctct tgccgcagcc
     1921 gctcggcccg acgagcaccg tgagctggcc cggccggatg gccaggttga ggttccggta
     1981 gagccacgga tgctgctccg agtagcggaa ggagaccgcc ttcagctcga tctgccccgc
     2041 ctgcgcgctg ctcgctcgct gcggcacgag cgcgtgcggc tccgccggca tgtccatgat
     2101 gtctcccagg cgccgcaccg ccacccgcgc ctgctggaac tgctgccaca ggccgaccaa
     2161 ccgcagcagc ggctgcgaca ggcgcgacgc gaacatctgg aacgcgacca gcatcccgat
     2221 ggtgaacgcg ccggcgccgg cggccgcccg cgcggagctg tcgatcacga ggagcgcccc
     2281 catcaccagg atggcgagcg tcatgacctg ctccagcgtg ttcgcgagca cgttgtaggt
     2341 gttggcgagc tggcgcgtct cgaagccggc ggcaagatac tgcgcgagct gatcgccgta
     2401 gcgctcctcg agaaccggct cgagctgaag cgacttcacg gtcgccatgc ccgagacgta
     2461 ttcggtgacg aacgccgtat tgcgcgcacc ggtgaggaac tggcggtcga gcttcacgcg
     2521 aaacatcggc gtcacggcca ggctcaggat cgcgatcgcc agcaggaccg cgagggcgat
     2581 cagcgtcagc tgccagctgt accagaacat caccgccagg aagacgaaca ggaacgggca
     2641 gtcgagcagc agcgcgaccg ccgccccgga aacgaagtcc cggatctgct cgacgccctg
     2701 cagccgcgcg accagcacgc cggtcgtgcg gtgctcgaag tacggcatcg gcaggcgaaa
     2761 caggtgccgg aacaccgact ggccgagcac cgcgtcgacc cggttcccgg tgtgcacgac
     2821 caagtattgc cgaagccatg tcatgacggc gccgaacagc atgaacatcc ccagcgccac
     2881 gcccacggcc cagagcgtgc tctgcgtgtg gtgcacgatc accttgtcga tcaccacctg
     2941 cgtgaagagc ggcgtggcga gccccgcgac ctggatcgcc agggacgcga cgagcacgtc
     3001 gcgccagatg ggcttgtgcc ggagcagttc ggggacgaac cagccgaagc caaaggcggc
     3061 gggccgggcg cgcttggcgt cggggtcagc gggctcgttg aggtcgcgcc gcagaagcac
     3121 cagcaccggg acgaaggctt cgtcaaatct tgcaagagga acgctaacgg ggtcccgcga
     3181 ggacgacttg aagaccacgg cctgaccggt atcgcacttc gcgacgatcg cgaccctgag
     3241 ctgcggctcg cccgattcgg cctcggcgcg ctcgaacgcg atgcacgggc acgtggtgcg
     3301 cgcgccgatc gacgcggtgc tcgcctggcc ggcgtgaaag ccgagcgcgc cgagcgcgcc
     3361 gagcagcttc tctacggagt acggcggggg aaactgcctc aggaacagat cggcatcaaa
     3421 ggggacacgg tgcaatgcgc aaagggtgcc gagcacccag acgaaatcgt ccttgggcac
     3481 ggcaccctgc gcaccgcccc ctgcggtcgg ctgggtcgcc acggttcccc tttgcgcaga
     3541 cagcctagac cacggccggc ctcctggcat ccaccatacg gtctagaccg cccggactaa
     3601 atggggacag acctcatttt cctgcggccc agcatttctc ctggggcgac ccttccgggc
     3661 acgcggcgct cccgcggcaa atcctccgtt ggtagccttc ctgagctcgt cgaccaggtt
     3721 ctgatcgagc gcctcgtcaa acagccggcg atagggctcc gcaccgggaa acgcggggtg
     3781 cggccggatc aggggatccg ctcttccgtc cgcgtttgcg cgatagctcg accaggggta
     3841 ctcccgcggg tgccgcacca taccggcgct gacagggttc agttccacgt accgataaca
     3901 ggcgagcgca tagcgctccg aatccaccag gcacgagcgg aagcggctgt cccacagact
     3961 gcccgtgcgc cggtgggcgc ggttgatgtg ctgcgtgtag cactgtgcca gggacttcat
     4021 catcagcgcg ctgccgctat cggtttgcgg cgtcagcaga aggtgcacgt ggttggtcat
     4081 gagacagtat gcgtgcacgg tgcatgcgtg ctcgcgggcg aagtagccga gcaggccgag
     4141 gtataaggca tagtcttcgc caccgaaaaa gcacgcttgc cggttgtgtc cccgctgcct
     4201 gatatgcaag gcgacacccg ggaccacgag gcgaccggtt ctgggcatgc ccttgaaacg
     4261 gccacacccg tcgcccggtt gacagcgggg aagaaaaatg aggtctgtcc ccatttaaaa
     4321 tgccgcatgg ccgagcgctg ggacaacccg atgggcacgg acggcttcga gttcatcgag
     4381 tacgcagccc cggacccgca ggtcatgggg gcgcttttcg agcgcttggg gttcagggcc
     4441 gtcgcccgcc acaggcacaa gaacgtggtg ctgtatcggc agggtggcgt caacttcatc
     4501 gtcaacgcgg agccggactc gttcgcgcag cgctttgccc gattgcacgg gccgagcatc
     4561 tgcgccatcg cgtttcgcgt gcaggacgcg aagagggcct acgagcgcgc ggtagatctc
     4621 ggcgcctggg gttacgctgg aaccgccggg ccgggggaac tcaacatccc ggcgatcaag
     4681 ggcatcggcg attccctgat ctatctcatc gaccgctggc gcggcaaggg cggcaagaaa
     4741 gagggcgaca tcggcaacat cggcttctac gacgtcgact tcgagccgct ctccggcgct
     4801 gagctaaatc ctgccgggta cggcctcacg gtcatcgacc acctcacgca caacgtgcac
     4861 aaggggcgca tgaaggagtg ggccgacttt tacgagcggc tgttcaactt caaggagatc
     4921 cgctacttcg acatcgaggg ccaggtgacc ggcgtgaagt cgaaggccat gaccagcccc
     4981 tgcggcaaga tccgtatccc gatcaacgag gaaggcaccg aaaaagcggg ccagatccag
     5041 gagtacctgg acctgtacca cggcgagggc atccagcacg tggcgctcgg ctcgaccgac
     5101 ctgttcagga cggtggacgc gctgcgcgcg aacggcgtga agctcctcga caccgtggac
     5161 acctactacg agctcatcga caagcgcatt ccgggccacg gcgagaacat cgccgcgctg
     5221 aagaagagga agatcctcgt cgacggcaag aagggcgcgc tcctgctgca gatcttctcc
     5281 gagaaccagc tcggcccgat cttcttcgag ttcatccaga ggaagggtga cgaaggcttc
     5341 ggcgagggca acttcaaggc actgttcgaa tccatcgagc tcgaccagat gcgccgcggc
     5401 gtccttaaat agggacagac ctcattttca acatagcgga ataatgaggt ctgtccccat
     5461 ttaatatgcc agcagaatat gcgctatcag tccgggttcg gtaacacctt ttcgtccgag
     5521 gcggagaaag gcgcgctgcc ggttgggcag aattcgccgc agaagacacc cagaggtctt
     5581 tatcccgagg tgctctcggg cagcgcgttc accgcgccgc ggcacgaaaa tctttccagc
     5641 tggctttacc gcctgcgtcc ttcggcgatg cacggtcctt tcaagcaact gggccaggg
//
LOCUS       DBNHKE010000250         7522 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1320817, whole genome shotgun sequence.
ACCESSION   DBNHKE010000250 DBNHKE010000000
VERSION     DBNHKE010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1320817"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="AC16O9_16895"
     CDS             complement(<1..1634)
                     /locus_tag="AC16O9_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HIR6518334.1"
                     /translation="MRLHQIRLSGFKSFVDPTSIHFPGQLVGIVGPNGCGKSNVIDAV
                     RWVLGETRASALRGDSMQDVIFNGSGNRKPLARASVELMFDNSLGRAGGQWSQYAELS
                     VKRVLQRDGESSYYINNQHVRRRDVTDMFLGTGLGPRAYAIIEQGMISRVIEAKPDEL
                     RVFLEEAAGVSRYKERRKETEHRLEDTRANLSRVTDIRLELGAQIEKLDAQAKVATRY
                     QEIQAELQLKQQLLWFLRRRDAAAERARHAQEIASQANALEAGTATLREIESRLETSR
                     AAHYAAGDGMNAAQAALYAANAEVARLESELRHVEETRQRLEFQHAERRTQLGAWREQ
                     RSQLTQALHLWAGRAGGARQRVADAQTRAAQEAGRLPQAEQAFRQAQEQQSECRSQLL
                     RAESRAQLEQSNLEHLERALEALGQRRERLLAERRALQQPDAAALAAQEARRGAGDTA
                     IAQAEDTLNALQEECDALEAERAAAAERLSAASHERASAAAQLATLEQIQAAAEDNAP
                     LRDWLERNALQALPRLWQQVRIEPGWEVAVESVLRE"
     gene            1708..2082
                     /gene="queF"
                     /locus_tag="AC16O9_16900"
     CDS             1708..2082
                     /gene="queF"
                     /locus_tag="AC16O9_16900"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR6518335.1"
                     /translation="MPSRPSKRLQTFRNPSPRRDYRIHMQLPEFTCLCPLTGQPDFAV
                     LELEYVPDRLCVELKSLKLYAWSFRDEGAFHEAVTNRIADDLVRALRPRYLRLTARFN
                     VRGGIATSVVAEHRKRGWKPAA"
     gene            2079..3245
                     /gene="dapC"
                     /locus_tag="AC16O9_16905"
     CDS             2079..3245
                     /gene="dapC"
                     /locus_tag="AC16O9_16905"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 -
                     succinyldiaminopimelate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR6518336.1"
                     /translation="MNPLLGRLQPYPFEKLRALLEGVTPNPALRPISLQIGEPKHATP
                     ELVKAALAASLDGLAAYPATAGTPALREAIAAWLTRRYALKAIDAATQVLPVNGSREA
                     LFAFAQTVVDPAGGTARVVCPNPFYQIYEGAALLAGAAPVYLNDGMAFDQIDWRGVQL
                     AYVCSPGNPTGRVLAPEDWKLLFELADRHGFVIASDECYSELYYDELNPPLGALQAAQ
                     QLGRAGFERVVAFSSLSKRSNAPGMRSGFVAGDATILKRFLLYRTYHGSAMSLPVQQA
                     SIAAWRDEEHVRANRRLYAEKYRAVLPLVAAPLATEMPQGGFYLWVRTPMDDAAFARE
                     LQRQYNVLVLPGSYLARDAHGANPGRNHVRIALVAPLDACVEAMQRIGQFARAL"
     gene            3255..4076
                     /gene="dapD"
                     /locus_tag="AC16O9_16910"
     CDS             3255..4076
                     /gene="dapD"
                     /locus_tag="AC16O9_16910"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR6518337.1"
                     /translation="MEQAQRIIDQAWEERAGLSPAKAPKPLREAIEFALAGLDAGSLR
                     VAEKRAGAWVTHQWLKKAVLLSFRLEDNRVMAGGFTNYYDKVGAKFSAYDAAAFGRGG
                     FRVVPPAMARRGCFIARNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPVQANPVIIEDNCFIGARSEVVEGVIVEENSVLSMGVFLGQSTKILNRE
                     TGEVSYGRVPAGSVVVAGTLPSGDGRCALYCAVIVKRVDAKTRAKTSLNDLLRGD"
     gene            4110..5306
                     /locus_tag="AC16O9_16915"
     CDS             4110..5306
                     /locus_tag="AC16O9_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR6518338.1"
                     /translation="MSAMKRLFQLMSDKKASDIFLSIGAPITIKINGVAMPVNQTILT
                     PSAVQTLLYEVLNEHQVREFEEELELNTAYTMEGVGTFRISAFRQKGTPAVVVRYIPG
                     NVPPLDTLGLPPVVKDVVMQKRGLILMVGATGSGKSTSLAAMIDHRNAERSGHILTLE
                     DPVEFIFKNRKSIVNQREVGTDTKAFPIALKNALRQAPDVILIGEIRDRDTMSMALQY
                     AQSGHLCMATLHANNSYHAMNRIISFYPLENRPTLLLDLSASLQAVISQRLVRTKAGQ
                     RRPACEVLLNTRHISELIEKGEINEMREAMEKSMAPGSQTFEQSLFKLFVDGLITQDE
                     AMLNADSATNMLWLINQATAGQITSGQMSVPVKPLTVQKDDDKQEGLLSGNGASFDGI
                     KIDTTT"
     gene            5308..6435
                     /gene="dapE"
                     /locus_tag="AC16O9_16920"
     CDS             5308..6435
                     /gene="dapE"
                     /locus_tag="AC16O9_16920"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR6518339.1"
                     /translation="MKPALELARELIACRSVTPADGGCQALIARRLAEVGFRIEDASV
                     NGVTNLWARRGEARPLVCFAGHTDVVPPGPLDKWLTDPFAPTERDGKLYGRGAADMKS
                     SLASIVVAIEQFVAERPAHAGSIALLLTSDEEGPSVDGTARVVERLKARGETIDYCIV
                     GEPSSSADLGDTVRVGRRGSLTARITVRGVEGHVAYPEQVKNPIHLLAPALAELAASE
                     WDRGNESFPPTSFQVSNLTAGTGVENVVPGAAQLICNFRFSTASSDASLRARLEDVLR
                     KHKLDYSISWTLGGKPFLSSRGRLIEAVRGAVQAHTGRAPQLSTGGGTSDGRFIIDIC
                     PEVIELGPVNATIHKLNEHVELDALERLPRIYLDTLRALLH"
     gene            6432..7271
                     /gene="prmB"
                     /locus_tag="AC16O9_16925"
     CDS             6432..7271
                     /gene="prmB"
                     /locus_tag="AC16O9_16925"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6518340.1"
                     /translation="MTLGELVRDAARRLRRAPLHYGHGTTNARDEAAFLVLRGLGLPF
                     EAPADRPVTSRERKRIERLLMRRIVERIPVPYLLQEAWLADEPFYVDRRAIVPRSHIA
                     AMLQQRLRPWLRRPARRVLDLCTGTGCLAVLAAKAFPRARVDAADLSRAALAVAGRNL
                     TLHRLRRRVRLVRSDLFAGLGQRRYDLIVANPPYVDARAMRALPREYLHEPRLALAGG
                     ADGLALVRRILGQAGAHLAPRGILVCEVGRGRRLLERACPRTPFAWPAAQVFVLSRED
                     LPA"
     gene            complement(7277..7351)
                     /locus_tag="AC16O9_16930"
     tRNA            complement(7277..7351)
                     /locus_tag="AC16O9_16930"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7317..7319),aa:His,seq:gtg)
     gene            complement(7369..7445)
                     /locus_tag="AC16O9_16935"
     tRNA            complement(7369..7445)
                     /locus_tag="AC16O9_16935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7409..7411),aa:Arg,seq:tct)
BASE COUNT         1142 a         2637 c         2515 g         1228 t
ORIGIN      
        1 ctctcgcgca gcaccgattc caccgccact tcccagcccg gctcgatgcg cacctgctgc
       61 cagaggcgcg gaagcgcctg cagggcgttg cgttcgagcc agtcgcgcag cggcgcgttg
      121 tcctccgccg ccgcctggat ctgctccagg gtcgcgagct gcgccgcggc gctcgcgcgc
      181 tcgtgcgacg ccgccgagag ccgctcggcc gccgcggcgc gctcggcctc gagcgcgtcg
      241 cactcttcct gcagggcgtt cagcgtgtcc tcggcttggg cgatggccgt gtcgcccgcg
      301 ccgcggcgcg cttcctgcgc cgcgagggcc gcagcgtccg gctgctgcag cgcccggcgc
      361 tcggccagca ggcgttcgcg ccgctgccct agcgcttcca gcgcgcgctc gaggtgctcg
      421 aggttcgact gttccagctg ggcgcggctt tcggcgcgca gcagctggct gcggcactcg
      481 ctctgctgct cctgcgcctg tcggaacgcc tgctcggcct gcggcagccg ccccgcttcc
      541 tgcgccgcgc gcgtctgcgc gtccgccacc cgctgccgcg cgccacccgc gcgcccggcc
      601 cacaggtgca gcgcctgcgt cagctgcgag cgctgctcgc gccaggcgcc gagctgcgtg
      661 cgccgctcgg cgtgctggaa ctcgagccgc tggcgcgttt cctcgacgtg gcgcagctcg
      721 gattcgaggc gcgccacctc ggcgttggcg gcgtagagcg ccgcttgcgc cgcgttcatg
      781 ccgtcgcctg cagcgtagtg cgccgcgcgt gacgtctcca ggcggctttc gatctcgcgc
      841 aacgtggcgg tgcccgcctc cagcgcgttc gcctggctgg cgatttcctg cgcatggcgc
      901 gcgcgctcgg cggcggcgtc gcgccgccgc aggaaccaca gcagctgctg cttgagctgc
      961 agctcggcct ggatctcctg gtagcgcgtg gcgacctttg cctgcgcgtc gagcttctcg
     1021 atctgcgccc cgagctcgag ccggatgtcg gtgacgcgcg acaggttggc gcgcgtgtcc
     1081 tccagccggt gctcggtctc cttgcgccgc tccttgtagc gcgagacgcc cgccgcctcc
     1141 tcgaggaaca cgcgcagctc gtcgggcttg gcctcgatca cgcgcgagat catgccctgc
     1201 tcgatgatgg cgtacgcgcg cgggcccagg ccggtgccga ggaacatgtc ggtgacgtcg
     1261 cgccggcgca cgtgctggtt attgatgtag taagacgact cgccatcgcg ctgcagcacc
     1321 cgcttcaccg agagctcggc gtactggctc cactggccgc cggcgcgccc cagggagttg
     1381 tcgaacatga gctccacgct ggcccgcgcg agcggcttgc gattgcccga gccgttgaag
     1441 atcacgtcct gcatcgagtc gccgcgcagc gccgaggcgc gcgtctcgcc cagcacccag
     1501 cgcaccgcat cgatcacgtt cgacttgccg cagccgttcg gaccaacgat gccgacgagc
     1561 tgacccggga agtggatcga ggtgggatcg acgaaggact tgaagcctga gagacggatc
     1621 tggtggagtc gcacggagcg tgttctcgtt tcgaaaatga taccatctgc gcgctcgcaa
     1681 ttcgccgcgc cacgccgtaa ttttccgatg ccctcgcgtc ccagcaagcg cctgcagacg
     1741 ttccgcaatc cgtcgccgcg gcgcgactat cgcatccaca tgcagctgcc cgagttcacc
     1801 tgcctgtgcc cgctcaccgg ccagccggac ttcgccgtgc tggagctgga atacgtgccg
     1861 gaccggctgt gcgtcgagct gaagtcgctc aagctctatg cctggtcgtt ccgcgacgag
     1921 ggcgcgtttc acgaagccgt caccaatcgt atcgcggacg acctggtgcg cgcgctgcga
     1981 ccgcgctacc tgcgcctcac cgcgcgcttc aacgtgcgcg gcggaatcgc taccagcgtg
     2041 gtcgccgagc accgcaagcg cggctggaag cccgccgctt gaatccgctg ctcgggcgcc
     2101 tgcagcccta cccgtttgag aaactgcgcg cgctcctcga gggcgtcacg cccaacccgg
     2161 cgctgcggcc gatcagcctg cagatcggcg aacccaagca tgccacgccc gagctggtga
     2221 aggcagcgct cgcggcatcg ctcgacggcc tggctgctta tcccgcgacc gcgggcacgc
     2281 cagcgctgcg cgaagcgatc gcggcatggc tcacgcggcg ctatgcgctg aaggcgatcg
     2341 atgccgcgac gcaggttttg ccggtgaacg gttcgcgcga ggcgttgttt gcctttgcgc
     2401 agaccgtcgt cgatccggca ggcggcacgg cccgggtcgt atgccccaat ccgttctacc
     2461 agatctacga aggcgccgcg ctgctcgccg gcgccgcgcc ggtctacctg aacgacggca
     2521 tggcgttcga ccagatcgac tggcgcggcg tgcagctcgc ctatgtgtgc tcgccgggca
     2581 accccacggg gcgcgtgctg gctccggagg actggaagct tctgttcgag ctcgccgacc
     2641 ggcacggctt cgtcatcgct tccgacgagt gctactcgga gctctattac gacgagctga
     2701 acccgcccct tggcgcgctg caggcggcac agcagctcgg gcgtgccggg ttcgagcgcg
     2761 tggtggcctt ttccagcctg tccaagcgct ccaacgcgcc cggaatgcgc tcgggcttcg
     2821 tcgcgggcga cgcaacgatc ctcaagcgct ttctgctcta tcgcacctac cacggctccg
     2881 ccatgagcct accggtgcag caggcctcga tcgccgcctg gcgcgacgag gagcacgtgc
     2941 gcgccaaccg gcgcctgtac gcggaaaagt accgtgcggt gctgccgctg gtggccgcgc
     3001 cgctcgccac cgagatgccc cagggcggct tctacctctg ggtgcgcacg cccatggacg
     3061 acgcggcctt cgcgcgcgag ctgcagcgtc aatataatgt gctggtgctt ccggggagct
     3121 atcttgcgcg cgacgcgcac ggcgccaatc ccggccgcaa ccacgtgcgc atcgccctcg
     3181 tcgccccgct cgacgcatgc gtcgaggcga tgcaaagaat cgggcagttt gcccgcgcac
     3241 tctgacgagg agctatggaa caagcgcagc gcatcatcga ccaggcctgg gaagagcgtg
     3301 ccggcctctc gcccgccaag gcgcccaagc cgctgcgcga ggcgatcgag ttcgcgctcg
     3361 ccgggctcga cgccggatcg ctgcgcgtcg cggaaaagcg cgccggcgcc tgggtgacgc
     3421 atcaatggct caagaaggcg gtgctgctct cgttccgcct cgaggacaac cgggtaatgg
     3481 cggggggatt cactaactat tacgacaagg taggggcgaa gttctcggcc tacgacgcgg
     3541 cggccttcgg ccgcggcggc ttccgcgtgg tgccgccggc gatggcgcgc cgcggctgct
     3601 tcatcgccag gaacgtggtg ctcatgccct cgtacgtgaa cattggcgcc tacgtcgacg
     3661 agggcacgat ggtggacacc tgggccaccg tcggctcctg cgcgcagatc ggcaagaacg
     3721 tgcacctctc gggtggcgtc ggcatcggcg gtgtgctcga gccggtgcag gccaacccgg
     3781 tgatcatcga ggacaactgc ttcatcggcg cgcgctcgga agtcgtggag ggggtgatcg
     3841 tcgaggagaa cagcgtgctg tcgatgggcg tgttcctcgg ccagagcacc aagatcctca
     3901 accgcgagac cggggaagtg agctacgggc gcgtgccggc cggctcggtg gtggtcgccg
     3961 gcaccctgcc ctcgggcgac ggccgctgcg cgctgtactg cgcggtgatc gtcaagcggg
     4021 tcgacgcgaa aacacgggcc aagacgagcc tgaacgattt gctgcgaggg gattaaacta
     4081 gcggggtagc actgccccgg aggtgtgcga tgtcggcgat gaaacgcctg ttccagttga
     4141 tgtccgacaa gaaggcctcg gatatctttc tgtccatcgg tgcgccgatc accatcaaga
     4201 tcaacggcgt ggcgatgccc gtgaaccaga ccatcctcac gcccagcgcc gtgcagacgc
     4261 tgctctatga ggtgctgaac gagcaccagg tgcgcgagtt cgaggaggaa ctggagctca
     4321 acaccgccta taccatggaa ggggtgggca ctttccgtat cagcgctttt cgccagaagg
     4381 gcacgcccgc ggtggtggtg cgctacatcc ccggcaacgt gccgccgctt gacaccctcg
     4441 gtctgccgcc ggtggtcaag gacgtggtga tgcagaagcg cggcctgatc ctgatggtgg
     4501 gtgccacggg ctccggcaag tcgacctcgc tcgcggcgat gatcgaccac cgcaacgccg
     4561 agcgctcggg ccacatcctg acgctcgagg acccggtcga gttcatcttc aaaaaccgca
     4621 agtcgatcgt caaccagcgt gaagtcggca ccgataccaa ggcgtttccg atcgccctca
     4681 agaacgccct gcgccaggcg ccggacgtca tcctgatcgg cgagatccgc gaccgcgaca
     4741 cgatgtcgat ggcgctgcag tacgcgcagt ccggccacct gtgcatggcg acgctgcacg
     4801 cgaacaacag ctaccacgcc atgaaccgga tcatcagctt ctacccgctg gagaaccgcc
     4861 ctacgctgct gctcgacctt tcggcctcgc tgcaggcggt gatctcgcag cgcctggtgc
     4921 gcaccaaggc ggggcagcgc aggcccgcct gcgaggtgct gctcaacacg cgccacatct
     4981 ccgagctgat cgagaaaggc gagatcaacg agatgaggga agcgatggaa aagagcatgg
     5041 cgcccggctc gcagacgttc gagcagtcgc tgttcaagct gttcgtcgac ggcctcatca
     5101 cccaggacga ggcgatgctg aacgcggact cggccaccaa catgctgtgg ctgatcaatc
     5161 aggccaccgc cggccagatc acgtcgggcc agatgtccgt gccggtgaag cccctgaccg
     5221 tgcagaagga cgacgacaag caggagggcc tgctctccgg caacggcgcg tcgttcgacg
     5281 gcatcaagat cgatacgacg acctgaagtg aagcccgctc tcgagcttgc ccgcgagctg
     5341 attgcttgca ggtcggtcac gcccgcggac ggcggctgcc aggcactcat cgcacggcgc
     5401 ctcgccgagg tgggttttcg catcgaggac gcaagcgtca acggcgtcac caacctgtgg
     5461 gcgcggcgcg gggaggcgcg gcccctggtg tgcttcgccg ggcacaccga cgtcgttccg
     5521 ccgggcccgc tcgacaagtg gcttaccgat cctttcgcac cgaccgaacg tgacggcaag
     5581 ctctatggac gcggtgccgc ggacatgaag tcctccctgg ccagcatcgt ggtcgccata
     5641 gagcagtttg tcgcggagcg gccagcgcac gccggctcta tcgccctcct gctcacctcg
     5701 gacgaggaag gcccctcggt ggacggtacc gcgcgcgtgg tcgagcggct caaggcgcgc
     5761 ggcgagacca tcgactactg catcgtcggc gagccttcct catctgccga tctcggcgac
     5821 acggtgcgcg tcgggcggcg cggctcgctc acggcgcgca tcacggtgcg cggcgtcgag
     5881 ggtcatgtcg cctacccgga gcaggtgaag aacccgatcc acctcctcgc ccccgccctc
     5941 gccgagctcg cggccagcga gtgggaccgc ggcaacgagt cgttcccgcc gaccagcttc
     6001 caggtctcga acctcaccgc cggcacaggc gtggagaacg tcgtgccggg cgccgcgcag
     6061 ctgatctgca acttccgctt ctccactgcg agcagcgacg cttcgttgcg cgcgcgcctc
     6121 gaggacgtgc tgcgcaagca caagctcgac tacagcatca gctggacgct cggcggcaag
     6181 ccctttctca gcagccgcgg ccgcctgatc gaggccgtgc gaggcgccgt gcaggcgcat
     6241 accggccgcg cgccgcagct gtccaccggc ggcggcacct cggacgggcg cttcatcatc
     6301 gacatctgcc ccgaggtcat cgagctcggc cccgtcaacg ccaccatcca caagctgaac
     6361 gagcacgtcg agctcgacgc cctggaacgg ttgccgcgca tctatctcga cacgctgcgc
     6421 gcgctgctgc attgacgctc ggcgagctgg tgcgggatgc cgcccggcgg ctgcgccggg
     6481 cgccgctgca ttacggacac gggacgacga acgcccgcga cgaggcggca tttctggtcc
     6541 tgcgcggctt gggcctgccc ttcgaagcgc ccgccgatcg tcccgtcacg tcgagggagc
     6601 gcaaacgcat cgagcgcctg ctcatgcgcc gcatcgtcga gcgcattccg gtgccctatc
     6661 tcctgcagga ggcgtggctt gccgacgagc cgttctacgt cgaccggcgc gccatcgttc
     6721 cacgctcgca tattgcggcg atgctgcagc agcggctgcg tccgtggctg cgccggcccg
     6781 cgaggcgcgt gctggacttg tgtaccggaa cgggttgcct tgcggtgctc gcggcaaaag
     6841 cgtttccgcg ggcacgcgtg gacgccgcgg acttgtcccg cgccgcgctc gccgtcgccg
     6901 gccggaacct caccctgcac cggctgcgcc gccgcgtgcg gctcgtgcgc tcagacctgt
     6961 tcgcgggact ggggcagcgg cgctacgacc tgatcgtcgc caatccgccg tatgtcgatg
     7021 cgcgggcgat gcgggcgttg ccgcgcgagt acctccacga gccgcgcctg gcgctcgccg
     7081 gaggcgccga cggcctggcg ctggtgcgcc gaatcttggg gcaagcgggc gcgcacctgg
     7141 ccccgcgagg aatcctcgtg tgcgaggtcg gtcggggccg caggttgctc gagcgcgcct
     7201 gcccgcgcac gccgttcgcc tggcccgcgg cgcaggtgtt cgttctgtcg cgcgaagact
     7261 tgccagcctg agaaagtggg gtgaccgatg ggactcgaac ccacaacaac tggaatcaca
     7321 atccaggact ctacctttga gctacggtca ccgtcgtcgc tgcagttctg gcctgcccgg
     7381 cgggagtcga acccacaacc cccagcttag aaggctggtg ctctatccgg ttgagctacg
     7441 ggcagatgtt ggttacaaga tatcacatcg agtaaatcgg gctgacttta attattagcc
     7501 tgttgatacg tgatatcaac ag
//
LOCUS       DBNHKE010000251         5413 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1373519, whole genome shotgun sequence.
ACCESSION   DBNHKE010000251 DBNHKE010000000
VERSION     DBNHKE010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1373519"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="AC16O9_16940"
     CDS             <1..193
                     /locus_tag="AC16O9_16940"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR6518341.1"
                     /translation="TGIRAPANWHGVWVVNLRSGKPQLEFSASLAELLEARGIRRTHL
                     TLTDERGLAAATVILECDA"
     gene            181..1209
                     /gene="nagZ"
                     /locus_tag="AC16O9_16945"
     CDS             181..1209
                     /gene="nagZ"
                     /locus_tag="AC16O9_16945"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR6518342.1"
                     /translation="MRRVRARLPLGPAVIDPAGTILGEDDVRRLRHPAVGGVILFARN
                     FESPEQLQALTRDIRALREPQLLIGVDHEGGRVQRFKEGFTALPPMRALGQLWDRDRE
                     AARAAARAAGYVIGAELAAHGLDFSFTPVLDLDYGASSVIGDRAFHYDPLAVAALGAE
                     LVRGLADGGMGAVGKHFPGHGYATADSHVAVPTDDRPLAEILRKDLVPYGPAIQAGLA
                     GVMPAHVIYSRVDAEPAGYSRVWLQEVLRGRLGFKGLVFSDDLSMEGASTAGGVAERA
                     QAALGAGCDMVLLCGDPEGQERLLHSLQGSTLASAERAEQMRHRGGRDLRRSVAFRDA
                     QGALGRLA"
     gene            complement(1206..1772)
                     /gene="efp"
                     /locus_tag="AC16O9_16950"
     CDS             complement(1206..1772)
                     /gene="efp"
                     /locus_tag="AC16O9_16950"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6518343.1"
                     /translation="MAKVSAVEIRVGNLIEWDKRVWRVLKCYHVHVGGRGGAFMQVEM
                     KDIESGTKTNQRFRTEDKVERAFVDARAMEYLYQDGDSYVFMDKENYEQLSLPAEFLE
                     GQAGYLLPNAEVQINFYNGRAIGIAIPPSVVLTVTDTEPGIRNATATNTFKPAKLETG
                     ITVQVPPFINKGERIKVDTNEGTYMERA"
     gene            1845..3611
                     /gene="uvrC"
                     /locus_tag="AC16O9_16955"
     CDS             1845..3611
                     /gene="uvrC"
                     /locus_tag="AC16O9_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR6518344.1"
                     /translation="MDDAKFDAQRFVAGLPNLPGVYRMLGAAGEALYVGKARDLKKRV
                     GSYFQKNAGSPRIRMMLSQLARIETTVTRSEDEALLLENNFIKSLAPRYNIVFRDDKS
                     YPYLMITAHSFPRLGFHRGARDARSRYFGPFPHAHAVRESIQLLQRVFRLRTCEDTVF
                     QNRSRPCLLHQIRRCTAPCTGLIGEGAYAEDVEHATLFLRGREDDVARKLTERMQAAA
                     RARHYEQAAVYRDQVRALSRVQARQYVESNRGVDADVVACAEQDGAGCVNLAMIRNGR
                     HVGDRSFFPSNAAAAGPAEVLQAFLEQHYAEQPKPALVLVSHAVEFPAVVPTQGERRV
                     WVEMARKNAELALQQRAREKSAQEARLAALREALHLPDSTQRIECFDISHTMGEATVA
                     SCVVYDRHAMQKGEYRRYNVGDAAPGDDYAAMRQVLGRRFERVNAEGGKVPDLVLVDG
                     GKGQAAVARAVLAELGMNDVSVVGVAKGPERKPGLEELVIEADGAALSLPPTHPGLHL
                     VQQIRDEAHRFAVLGHRARRAKTRTASALTQIPGIGSERRQRLLTHFGGVREVQAAAV
                     DELMQVAGISRPLAERIYKALH"
     gene            3611..4177
                     /gene="pgsA"
                     /locus_tag="AC16O9_16960"
     CDS             3611..4177
                     /gene="pgsA"
                     /locus_tag="AC16O9_16960"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR6518345.1"
                     /translation="MPLNLPNLITWLRIILIPLLIGIFYLPDDWLSPSGKNASACAVF
                     VVAALSDWLDGYLARALDQTSAFGAFLDPVADKLIVAAALIVLLQLERVEPVIALIII
                     GREIAISALREWMAKLGQAGSVAVAFVGKVKTASQMVAIPLLLYHDALFGVISTQWVG
                     TLLINIAAVLTVISMVHYLRRALPYAGG"
     gene            4202..4277
                     /locus_tag="AC16O9_16965"
     tRNA            4202..4277
                     /locus_tag="AC16O9_16965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4235..4237,aa:Gly,seq:gcc)
     gene            4329..4402
                     /locus_tag="AC16O9_16970"
     tRNA            4329..4402
                     /locus_tag="AC16O9_16970"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4361..4363,aa:Cys,seq:gca)
     gene            4423..4509
                     /locus_tag="AC16O9_16975"
     tRNA            4423..4509
                     /locus_tag="AC16O9_16975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4457..4459,aa:Leu,seq:taa)
     gene            4894..5142
                     /locus_tag="AC16O9_16980"
     CDS             4894..5142
                     /locus_tag="AC16O9_16980"
                     /inference="COORDINATES: protein motif:HMM:NF041373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HIR6518346.1"
                     /translation="MRTLRWQINSILSQFDWLEIIRKRTCGAKTRRGTPCKIKTNTYR
                     CKYLGGLSTGPRTAEGKARSAKNLILAWQVQRSRKQTP"
BASE COUNT          887 a         1767 c         1844 g          915 t
ORIGIN      
        1 gaccggcatc cgcgcgccgg ccaactggca cggcgtctgg gtggtcaacc tgcgctcggg
       61 caagccgcag ctggaattct cggcatccct cgccgagctc ctcgaggcgc gcggcatccg
      121 ccgtacgcac ctgaccctga ccgacgagcg tggcctggcc gccgcaacgg tgattctgga
      181 atgcgacgcg taagggcgcg gttgccgctc gggccggcgg tcatcgatcc ggcgggcacg
      241 attctcggcg aggacgatgt ccgccgcctg cggcaccccg cagtgggcgg cgtcatactg
      301 ttcgcgcgca acttcgagtc gcccgagcag ctgcaggcgc tgacccggga catccgcgcc
      361 ctgcgcgagc cgcagcttct gatcggcgtg gatcatgagg gcggccgcgt gcagcgcttc
      421 aaggaggggt ttaccgcgtt gccgccgatg cgagcactgg gccagctatg ggaccgggac
      481 cgcgaggcgg cgcgcgcggc ggcccgagcc gccggctacg tgatcggggc ggaactggcg
      541 gcgcacggcc tcgatttcag cttcacgccg gtactcgacc tcgactatgg ggcaagctcg
      601 gtgatcggag accgcgcgtt tcactacgac ccgcttgccg tcgcggcgct gggagcggaa
      661 ctggtgcgcg gcctcgccga cggcggcatg ggtgcggtcg gcaagcattt cccgggtcac
      721 gggtacgcca ccgcggattc gcacgtcgcc gtgccgaccg acgatcgccc gctcgcggag
      781 atcctgcgca aggatctggt gccctacggc cccgccatcc aggcgggcct ggccggggtg
      841 atgcccgcgc acgtgatcta ttcccgggtc gatgccgagc cggcgggcta ttcgcgtgtc
      901 tggctgcagg aggtgctgcg cggcaggctg ggcttcaagg gcctggtgtt cagcgacgac
      961 ctgtcgatgg aaggggcgag cacggcgggc ggcgtcgccg agcgcgccca ggccgcgctc
     1021 ggtgcgggct gcgacatggt gctgctgtgt ggggatccgg agggccagga gcgcctgctg
     1081 cactccctgc aggggagcac cctggcctcg gcggagcgtg ccgagcagat gcgccatcgc
     1141 ggcggccgcg acctgcgacg cagcgtcgcc tttcgcgatg cgcagggcgc gctcggcagg
     1201 ctcgcctagg cgcgctccat gtaggtgcct tcgttcgtgt cgaccttgat gcgctcgccc
     1261 ttgttgatga acggcggcac ctgcacggtg atgccggttt ccagcttggc gggcttgaag
     1321 gtgttggtgg cggtcgcgtt gcggatcccg ggctcggtgt cggtcacggt gagcaccacc
     1381 gagggcggga tggcgatgcc gatggcgcgg ccgttgtaga aattgatctg cacctcggcg
     1441 ttgggcagga ggtagccggc ctggccttcc aggaactcgg cgggcagcga cagctgctcg
     1501 tagttctcct tgtccatgaa cacgtagctg tcgccgtcct gatacaggta ctccatggcg
     1561 cgcgcatcca cgaaggcgcg ctccaccttg tcctcggtgc gaaagcgctg attggtcttg
     1621 gtgcccgact cgatgtcctt catctccacc tgcatgaacg cgccgccacg gccgccgacg
     1681 tgcacgtggt agcacttgag cacgcgccat acgcgcttgt cccactcgat cagattgccg
     1741 acgcgaattt cgactgcgga gaccttggcc atggcgggat tgccggatgg aaaacgggcg
     1801 cgaattttac actgattccg aggcgcggcg ctagaatcgt ccccatggac gacgcgaagt
     1861 tcgacgcaca gcgcttcgtc gccggcctgc ccaacctgcc cggcgtctac cgcatgctcg
     1921 gtgccgcggg cgaggccctg tacgtaggca aggcgcggga tctcaagaag cgcgtgggtt
     1981 cgtatttcca gaagaacgcc ggcagcccgc gtatccgcat gatgctgtcc cagctggcgc
     2041 gcatcgagac cacggtgacg cgctcggagg acgaggcgct gctgctggag aacaacttca
     2101 tcaagagcct ggcgccgcgc tacaacatcg tcttccggga cgacaagtcc tatccgtacc
     2161 tgatgatcac cgcgcactcc tttccgcgct tgggctttca ccgcggcgcg cgcgacgcgc
     2221 gcagccgcta tttcgggccg ttcccgcacg cccacgccgt gcgcgaaagc atccagctgc
     2281 tgcagcgcgt gttccggttg cgcacttgcg aggatacggt gttccagaac cgctcccggc
     2341 cgtgcctgct gcaccagatc cggcgctgca cggcgccctg caccgggctg atcggcgagg
     2401 gcgcctatgc cgaggacgtg gagcacgcga cgctcttcct gcgcggacgc gaggacgacg
     2461 tggcgcgcaa gctgaccgaa cgcatgcagg cggcggcacg cgcgcggcac tatgagcagg
     2521 cggcggtgta ccgcgatcag gtgcgcgcgc tgtcgcgcgt gcaggcgcgc caatacgtgg
     2581 aatcgaaccg cggcgtggac gccgatgttg tggcctgcgc cgagcaggat ggcgcgggct
     2641 gcgtcaatct ggcgatgatc cgcaacggcc gtcacgtcgg cgaccgcagt ttctttccgt
     2701 caaacgccgc cgctgccggg cccgccgaag tgctgcaggc cttcctggag cagcattacg
     2761 ccgagcagcc aaagcccgcg ctggtactgg tgagccacgc ggtggagttc cccgccgtgg
     2821 tgccgacgca gggagagcgc cgcgtgtggg tggaaatggc acgcaagaac gccgagctcg
     2881 cgctccagca gcgcgcgcgc gagaagagcg cgcaggaggc ccgcttggcg gcgttacgcg
     2941 aagcactgca ccttcccgat tcgacgcagc gcatcgaatg cttcgacatc agccatacca
     3001 tgggcgaagc gacggttgcc tcctgtgtgg tctacgatcg gcacgcgatg cagaaaggcg
     3061 agtaccggcg ctacaacgtc ggcgatgcgg cgccgggcga cgattacgcg gcgatgcgcc
     3121 aggttctggg gcgccgcttc gaaagggtga acgccgaggg cggcaaggtc ccggacttgg
     3181 tgctggtgga cggtggcaag ggacaggcag ccgttgcgcg cgcggtactt gccgagctgg
     3241 gcatgaacga cgtcagcgtc gtgggcgtgg ccaaggggcc ggagcgcaag cccggactcg
     3301 aagagctggt catcgaagcc gacggcgccg cactcagcct gccgccgacg cacccgggat
     3361 tgcacctcgt gcagcagatc cgcgacgagg cgcatcgctt tgcggtcctc gggcatcgcg
     3421 cccggcgcgc caagacgcga accgcctcgg ccttgacgca gatccccggc atcggctcgg
     3481 agcgccgcca gcgcttgctg acccatttcg gcggcgtgcg ggaggtgcag gcggcggcgg
     3541 tggacgagct gatgcaagtc gcgggcatca gccgaccgct cgcggaacgc atctacaagg
     3601 cgctgcactg atgccgctca acctgcccaa tctcatcacc tggctgcgca tcatcctgat
     3661 tccgctgctg atcgggatct tctaccttcc cgatgactgg ctttccccga gcggcaagaa
     3721 cgcttcggcc tgcgcggtgt tcgtggtcgc ggctttgagc gactggctgg acgggtacct
     3781 ggcgcgcgcg ctcgaccaga cgtccgcgtt cggcgcattt ctcgatccgg ttgccgacaa
     3841 gctgatcgtg gcggccgccc tcatcgtatt gctgcagctg gagcgggtcg agccggtgat
     3901 cgcgctgatc atcatcggcc gggagatcgc catatcggcg ctgcgcgagt ggatggcgaa
     3961 gctcgggcag gccggcagcg tcgccgtggc attcgtcggc aaggtgaaaa cggcgagcca
     4021 gatggtggcc atccccctgc tgctctacca cgatgcgctt ttcggcgtca tcagcacgca
     4081 gtgggtgggg accctgctca tcaatatcgc cgcggtgctg acggtgatct ccatggtgca
     4141 ttacctgcgc cgcgcgctgc cgtacgcggg aggatgagct ataatccggg cccccaaaga
     4201 cgcgggagta gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg cgagttcgag
     4261 actcgtctcc cgctccagtt taggctaggg gaggccgcca ggccttccct gtttccgagg
     4321 catcccctgg cgggatggca gagtggtcat gcagcggcct gcaaagccgt ggacgccggt
     4381 tcgattccga ctcccgcctc cagactttct tcctacagcg ccgcccgggt ggcgaaactg
     4441 gtagacgcaa gggacttaaa atccctcggc cgcaaggccg tgccggttca agtccggccc
     4501 cgggtaccaa tctttagcga tagcagtcac taacggtact ttaattagtc cccgcaatag
     4561 tccccgtata cgccccggcg cttgaagggg gcgcctgagg cggaaactcg ggatcgccac
     4621 caatctcgcg cccgcctttc tggggcgtga gggaccgggg caggggggta gcatcctttt
     4681 tcggcttcga ccgcccccgg ttgtccaagg gttcccgaca ctgtgcccgg atgcggcgcg
     4741 ctcgattcga cacccacccc ctatgccgat tcctgcgcgg cgcgcgcaat tttctacagc
     4801 gtttacttca gagcgcgttt ctcggcggcg gtacagccct ttgcgctcga cagtttcagg
     4861 acctagagtt acggggtact aggagcgccc aagatgcgta ctctccgctg gcaaattaac
     4921 agcatcctta gccagttcga ttggctggaa atcattcgta agcggacgtg cggcgctaag
     4981 actcgacgcg gaacaccttg caagatcaag accaacacct accgctgcaa gtacctcggc
     5041 gggctatcca ccggcccccg aaccgccgag ggcaaggcca ggagcgcgaa gaatctcatt
     5101 ctcgcgtggc aggttcaacg gtctcggaag caaaccccat gacgcgcgcg cgaagtgcga
     5161 gcgtcgaagt cgccgaccac gaggacgcgg tgcgcgctgc gcttggcgtc ggcgctatgc
     5221 gcacgctgca ctgatgcggc tggggggcta gcgcccccca gacccccctc aaagaatcaa
     5281 aggctcaaat catccaatca tcaagcgaaa atcaccggcc gccacgtacg gcacgcgcat
     5341 gggagaagtt gaacttgctg tcgttgagca ggaagccatt gaagaaattg acgaaccacg
     5401 cgacgtcgac gat
//
LOCUS       DBNHKE010000252        14150 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1162102, whole genome shotgun sequence.
ACCESSION   DBNHKE010000252 DBNHKE010000000
VERSION     DBNHKE010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14150
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1162102"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..83)
                     /locus_tag="AC16O9_16985"
     tRNA            complement(3..83)
                     /locus_tag="AC16O9_16985"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            405..1016
                     /locus_tag="AC16O9_16990"
     CDS             405..1016
                     /locus_tag="AC16O9_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6518347.1"
                     /translation="MRTFLCRSVSGIAVALLAAPALAAQLENMQAFVGQAGLYVLLLV
                     AIVVAIYVYSMRSRDKRAAPLARIFAEGEAIHSVEPNTLVTDCVRAMAEAKIGALIVM
                     DGGRLVGIFTERDALNKVLAVGLDPRSTKVSQVMTKDPYSVPPTTTVGAAMQVVTQRR
                     FRHLPVVQDGKVLAVLSSGDLTHWLVKDQMGEIKELVDLAARS"
     gene            complement(1074..1149)
                     /locus_tag="AC16O9_16995"
     tRNA            complement(1074..1149)
                     /locus_tag="AC16O9_16995"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Lys,seq:ttt)
     gene            complement(1201..1872)
                     /gene="queC"
                     /locus_tag="AC16O9_17000"
     CDS             complement(1201..1872)
                     /gene="queC"
                     /locus_tag="AC16O9_17000"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR6518348.1"
                     /translation="MKAVVLVSGGLDSATALGWARREGYECYALSVDYGQRHGAELAA
                     AAKVAKRLGALEHRVVKLDLAQFGGSALTDKKIGVPDQGATSGIPVTYVPARNTILLS
                     LALAWAEVLQAQHVFMGANAVDYSGYPDCRPEYLKAYEAMANLATKAAVEGRRISIRA
                     PLVDLPKKEIVKLAMELGVDAAETVTCYQPDAAGRACGACDACRIRKKGFADAGIPDS
                     TRYQR"
     gene            complement(1869..2666)
                     /gene="ybgF"
                     /locus_tag="AC16O9_17005"
     CDS             complement(1869..2666)
                     /gene="ybgF"
                     /locus_tag="AC16O9_17005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR6518349.1"
                     /translation="MNAVRGVAAALLVALAGTAQAQLFGGDDQARRRIEEVRTETLLQ
                     LQQMEQRLGERMKALEATSIERRAILDLAGQLEALRADLAKQRGQIEVLIYQMEVAEK
                     RQKDLYVDIDTRLRKLESAIEQQAAVAARPPPEAGPSEEERRAYESALNQFKVGNYSA
                     AIDLLGQLLVKYPNGKLAPNAQYWIGMGYSGRRDYRNAIGALQKVIQQWPLDPKAADA
                     MLSIASAQEAMGDVKSAQATLQEVLAKYPGSTAADQARLRLQQSVKR"
     gene            complement(2668..3243)
                     /gene="pal"
                     /locus_tag="AC16O9_17010"
     CDS             complement(2668..3243)
                     /gene="pal"
                     /locus_tag="AC16O9_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR6518350.1"
                     /translation="MRILLSILIAATLAACATTETPGTQPPAGVEDRKPGAVQPAPGP
                     GTQGVPSKPVTGVDLTGGKPAPTPGVPPHRDPASVLAARSVYYDFDRYDIKDEFKPLI
                     EAHAAYLRGQPELKMLVQGNTDERGSREYNIALGQRRSEGVKRMLLLLGAREEQVEAV
                     SLGEEKPKELGHTEEAWTRNRRSDMLYTGEY"
     gene            complement(3258..4538)
                     /gene="tolB"
                     /locus_tag="AC16O9_17015"
     CDS             complement(3258..4538)
                     /gene="tolB"
                     /locus_tag="AC16O9_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR6518351.1"
                     /translation="MRNLLAAVLFAFAACSAHAQLSIEITGAGAQRIPIAIAPLAGEG
                     ALLDPSGATIGAIVRADLERSGLFRSLEIPRLVPQPTEASVVNYAEWRARLADALVLG
                     SVAARPDGRYEVRFRLYDVVKQTALGGVAYTLTREQVRATAHRIADYVYEKLTGEKGV
                     FSTRIAYVVKRGERYELQVADADGANEQAMLVSNEPIISPAWSPDGKRLAYVSFQNKK
                     PIVYVQDVEVPKQRIVANFKGSNSAPAWSPDGKRLALALTRDGNSQIYLIDPDGSNLQ
                     RFTRSAGIDTEPFFSPDGDWLYFTSDRGGSPQIYRMALRGGDAQRVTFEGGYNVSPRV
                     NPDGSSLAYIARNGGRFQLALLELATRQTQILTDSDLDESPSFAPNGRLILLATIVAG
                     RGVLTAVSADGRVKQRLSVTAGDVREPAWGPLNP"
     gene            complement(4554..5348)
                     /locus_tag="AC16O9_17020"
     CDS             complement(4554..5348)
                     /locus_tag="AC16O9_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6518352.1"
                     /translation="MAAKAASGLLFEAPREHFFGPSAMLALAVHVLLVAALMLGVHWQ
                     SRAPDVVMVELWQAPPQPVAQPAPPPKVKPPPKVEPPPPPAPKPEPRIEKPDIALKAK
                     PKPKPRPEPKPEPRPEPKPVAKPEPAPPPDETMAQRLLAEALQREDQRRAADRERQQI
                     RDQLLREQQAARNRAMQTWIDKIRGKIRGNIVLPPDIQGNPEALVLVTQLPTGEVLEA
                     KLVISSGHQAYDDAVMRAILKSSPLPKPDTPGLFERDLKLTFHPKD"
     gene            complement(5348..5776)
                     /gene="tolR"
                     /locus_tag="AC16O9_17025"
     CDS             complement(5348..5776)
                     /gene="tolR"
                     /locus_tag="AC16O9_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR6518353.1"
                     /translation="MPAPQRSRRPMHEINIVPYVDVMLVLLVIFMVTAPLITPGVVDL
                     PSVSKAANAPATVLEIVIREDKSVTLRVRDAQGALAEERRLARGELARAVRERQAKSP
                     DRPVLIAADKDVKYEAVLNVMDELQRQQIRRIGLLVQPAK"
     gene            complement(5779..6462)
                     /gene="tolQ"
                     /locus_tag="AC16O9_17030"
     CDS             complement(5779..6462)
                     /gene="tolQ"
                     /locus_tag="AC16O9_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR6518354.1"
                     /translation="MNVTTDLDIWILIMGASPVVKAVMALLVVVSFMSWLYIFSKWFT
                     IRRARSQTERFEREFWGGNDLNLLYQGAVNNRHSIGSLERIFESGFREFVKLRNQRPS
                     GDASDMVDGARRAMRATSQREMDHLERHLAFLASTGSVSPYVGLVGTVWGIMHAFRSL
                     ANVQQATLAQVAPGIAEALVATALGLFAAIPAVVAYNRYSYLIDRLSIRFESFMEEFS
                     NILHRQAVK"
     gene            complement(6467..6859)
                     /gene="ybgC"
                     /locus_tag="AC16O9_17035"
     CDS             complement(6467..6859)
                     /gene="ybgC"
                     /locus_tag="AC16O9_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR6518355.1"
                     /translation="MFSWPLRVYYEDTDFGGVVYYANYLRFFERARTEWLRSLGVDHL
                     RLAREDGLQFVVRRAEIDFLRGARLDDELSITVECTERKRTYLWLRQRALCGVEPMAE
                     GRVQVACVRRDDFKPTPIPAALAAQLGG"
     gene            complement(6860..7891)
                     /gene="ruvB"
                     /locus_tag="AC16O9_17040"
     CDS             complement(6860..7891)
                     /gene="ruvB"
                     /locus_tag="AC16O9_17040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6518356.1"
                     /translation="MAIESDRLIAAAPASPQEEQFERSLRPTRLADYVGQAKTRDQLA
                     IFIEAARGRNEALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAAL
                     LTNLEPRDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKLDLPPFTLV
                     GATTRAGMLTNPLRDRFGIVARLEFYNETELAAIVRRSAGLLEVALAEEGGVEIARRA
                     RGTPRVANRLLRRVRDYAQVKAAGAVSKSVAEAALGMLDVDVLGLDVMDRKLLLAVIE
                     KFGGGPVGVENLAHAIGEDADTIEDVLEPYLIQQGYLQRTPRGRVATLAAYRHFGLAA
                     PSGGHDLLA"
     gene            7962..8534
                     /locus_tag="AC16O9_17045"
     CDS             7962..8534
                     /locus_tag="AC16O9_17045"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR6518357.1"
                     /translation="MSAARALAAAFAALWASGVLAQAKAPDNGWYLGGGIRPVMPAIE
                     DSDIGYRHFGGYRFSRHWGVELGYSDLGRGPESTEPAFLAQQKTGAQTSAWTLAGTGV
                     LPIGEAFSVQGRLGFSVTSPDATFLAPSTGISSAFPRYRPTVLWGFGGQYDVTGSIGL
                     RLDYNNFGRLSDDPNGTRSDLWSINAVVRF"
     gene            complement(8538..9104)
                     /gene="ruvA"
                     /locus_tag="AC16O9_17050"
     CDS             complement(8538..9104)
                     /gene="ruvA"
                     /locus_tag="AC16O9_17050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6518358.1"
                     /translation="MIGRLTGKLVAKHPPQVMVDCNGVAYEVDVPMSTFYSLPATGEP
                     VTLHTHLAVREDAQALYGFSTLEERSAFRQLIRIAGVGARTALSVLSGLSVAELSQAV
                     AMQDAARLTKIPGIGKKTAERLLLELKGKLAEARPAGTTAAGADVLNALLALGYNDKE
                     ALAATRGLAPGLSVAEGIRAALKTLAKP"
     gene            complement(9101..9616)
                     /gene="ruvC"
                     /locus_tag="AC16O9_17055"
     CDS             complement(9101..9616)
                     /gene="ruvC"
                     /locus_tag="AC16O9_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6518359.1"
                     /translation="MRILGIDPGLQVTGFGVVDKNGQRLAYVASGCVKSGRGELAERL
                     KTLLEGLAEVIAAHRPQQVVVEKVFVNVNPQSTLALGQARGAAICAAVLAGLPVAEYT
                     ALQVKQSVVGNGQAGKSQVQHMVKRLLALAGDPSPDAADALACAICHAHGARLGRLST
                     RGLRMRRGRLA"
     gene            complement(9613..10341)
                     /locus_tag="AC16O9_17060"
     CDS             complement(9613..10341)
                     /locus_tag="AC16O9_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6518360.1"
                     /translation="MAGHSKWANIKHRKAAADAKKGKVFTRLIKEITVAAKLGGGDAA
                     TNPRLRLAMDKAREANMAKDSVLNAVKRGSGQLEGVSYEEVRYEGYGPGGAAVIAACL
                     TDNRTRTVAEVRHAFTKNGGNLGSDGSVAFLFKHCGQFVFAPGASEEKIMDAALEAGA
                     EDVQANDDGSIEVVCPPHEFLAVKAAFDKAGLKPELAEVTLKPTTEAALAGSDAQRMK
                     SLLDALESLDDVQDVYTTAVFEDA"
     gene            10360..11841
                     /locus_tag="AC16O9_17065"
     CDS             10360..11841
                     /locus_tag="AC16O9_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HIR6518361.1"
                     /translation="MPAPLLVAKNAKAELHLLPALANRHGLVTGATGTGKTVTLQTLA
                     EQFSRAGVPVFMADVKGDLSGMAKAGGGNAKVAERAKLLELELRHEACPVVFWDVFGK
                     RGHPVRATISDMGPLLLSRMLGLNDTQEGVLNLVFRIADEAGLLLLDVKDLRAMLQHV
                     GENAAQFTTHYGNVSAASIGAIQRGLLTLENQGAAQFFGEPMLNIADLMQVESGKGAV
                     NILAADQLMNSPKLYGTFLLWMLAELFEQLPEVGDPDKPKIVFFFDEAHLLFEDAPKA
                     LLDKIEQVVRLIRSKGVGVYFVTQNPLDIPESVLGQLGNRVQHALRAFTPKDQKAVKS
                     AAETMRPNPKLKIAEAITELGVGEALVSLLDEKGRPGITERAFVVPPGSQLGPLAEAE
                     RAAAISASPIAGHYEKTVDRESAYEKLKARADQVAQEQAAAKPKESSMASDILFGKTG
                     PRGGRQSQGLIEAMAKSAARTVGSQVGRELIRGVLGSLLGKRR"
     gene            11838..12734
                     /locus_tag="AC16O9_17070"
     CDS             11838..12734
                     /locus_tag="AC16O9_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6518362.1"
                     /translation="MSPAAYRGWGMLFALGGTVAFAFRPVLIKLGYEAAPVSATTLLF
                     LRMTLSLPFFAAVAWWLRHEPRLARRDWAGIVGLGFLGYYLASLLDFLGLQYVPAGLG
                     RLIMFLYPTLVVILSALFLARRPTGREIAALAMTYAGIALVLARQLGVEPEHRLFLWG
                     AALIFGSAMSYAVYLVTGSQLVKRVGSMRFTAYTMMVSTVPALVQFALLERPAALQLP
                     GEVWLYAILLATVCTVLPVFLVAEALKRIGANHFALIGGLGPVTTVLADFALLEGALG
                     PLQLLGGALVIAGVLFVSLKKG"
     gene            12740..13519
                     /locus_tag="AC16O9_17075"
     CDS             12740..13519
                     /locus_tag="AC16O9_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6518363.1"
                     /translation="MDLGIKGKNALVCAASKGLGKGCAMALAREGVNLVITARGKEAL
                     EATAEEIRKSTGAKVTSVAGDITTEEGRKAALAACPNVDILVNNAGGPPPGDFRNWSR
                     EDWIKAVDANMLTPIELIKATIDGMISRKFGRIINITSGAVKMPIAELGLSNGARTGL
                     TGFVAGLSRQVAQHNVTINGLLPGPFDTDRLRGNMEFNAKKLGTTAAELAKQRAAANP
                     SKRFGNIDEFGATCAFLASVHAGYITGQNVLMDGGAFPGTF"
     gene            complement(13520..>14150)
                     /locus_tag="AC16O9_17080"
     CDS             complement(13520..>14150)
                     /locus_tag="AC16O9_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6518364.1"
                     /translation="GAYLHQGQICMATGTVLAQRKIARDLTERLVAKAKHLPVGDPSA
                     GPCALGPIINGRQIERIDGIVKDAVAKGAVLRAGGTYDKLFYAPTVLENVTPAMRVYT
                     EEVFGPVLAVVPFDTDEEAIALNNATEYGLSTGVITQSVERAMNFTAKLKTGIIHVND
                     QTVADEPWVPFGGRGASGNGGRHGGVANWEEFTQWQWVTIQDKATPYPF"
BASE COUNT         2059 a         4903 c         4846 g         2342 t
ORIGIN      
        1 attggtaggc cgtggcggat tcgaaccgcc gaccaacgga ttaaaagctg gtatccgtta
       61 acgcaagttg ttgatttcgg cccgccctca cccgccaaaa cgcgccaaag ctcgccaaga
      121 aggttacgtt ctcgtcacgt tgttctcagc tgggacgtaa ctgtccgctt ccgacccaga
      181 accgcggttc gtggcatgcg attttcgccg ttccttgacc cgccgaccgc agccgtcaag
      241 agccctttat cgcatgcgga cagagcgatc gaacgaggcg gtgcgggcga ttgtcgatgg
      301 catcgggcga acgcacgccc gcggcgcggg tccaaggccc gtagggggca gcagcgggga
      361 agaggcgttg tggcggcaag cgccaggttt gaggaggata ggccatgaga acgtttcttt
      421 gtagatcggt gtccggtatc gctgtcgcgc tgcttgccgc tccggcgctt gctgcgcaac
      481 tggagaacat gcaggccttc gttggacagg cgggacttta tgtcctgcta ctggtcgcga
      541 tcgtcgttgc catctacgtt tactcgatgc gatcgcgaga caagcgtgca gcgcctttgg
      601 ccagaatatt cgcggaaggc gaagcgattc actcagttga acccaacacc ctggtcacgg
      661 attgtgttcg ggcgatggcg gaggcgaaga tcggcgccct gattgtgatg gatggcgggc
      721 gattggtagg catcttcacg gagcgagatg cgctgaataa ggtattggcg gtcggcctcg
      781 atccgcgcag cacgaaagtg tcacaggtga tgacgaaaga tccttatagc gtcccgccga
      841 cgacaaccgt tggcgccgcg atgcaggtcg ttacccagcg acggttccgg cacctgcccg
      901 tcgttcaaga cggcaaggta ctggccgtct tatcgagtgg cgatctaacg cattggttgg
      961 tgaaagatca aatgggcgag atcaaagagc tggtcgacct tgctgcccgg tcctgacttt
     1021 cgccgcagca gaaagcgaaa agggcctagc gttacgcaag gcccttgatg gattggtagg
     1081 ccgtggcgga ttcgaaccgc cgaccaacgg attaaaagtc cgctgctcta ccagctgagc
     1141 taacggcccc catgggaagg gcgcgatttt atcagccctc gcccccgctt cgccacccgt
     1201 tcagcgctga tagcgcgtcg aatcgggaat tccggcgtcc gcgaagccct tcttgcggat
     1261 gcggcacgca tcgcacgcgc cgcacgcgcg ccccgcagca tccggttggt agcaggtgac
     1321 ggtctcggcc gcatccaccc ccagctccat cgccagcttc acgatttcct tcttcggcag
     1381 atccaccagc ggcgcacgaa tcgagatccg ccgcccctcc accgccgcct tggtcgccag
     1441 gttcgccatc gcctcgtacg ccttcaagta ctcgggccgg caatccggat agcccgagta
     1501 gtccaccgcg ttcgccccca tgaagacgtg ctgcgcctgg agcacctccg cccaggccag
     1561 ggcaagggac agcaggatgg tgttccgcgc ggggacataa gtgacgggga tcccggaggt
     1621 cgccccctgg tccggaaccc caattttctt gtcggtgagc gccgagccgc cgaactgggc
     1681 gagatccagc ttcaccaccc ggtgctcgag ggcccccagc cgcttggcga ccttcgccgc
     1741 tgcagccaat tcagccccgt ggcgctgtcc gtaatctacc gagagagcgt agcactcgta
     1801 cccctcgcga cgcgcccagc cgagcgcagt cgcagagtcg agccccccgg atacgaggac
     1861 gacggccttc agcgcttgac cgactgctgc agccgcagcc gcgcctggtc ggccgcggtg
     1921 ctgccggggt acttggccag cacctcctgc agcgtcgcct gcgccgactt cacgtcgccc
     1981 atcgcctcct gggcgctcgc gatcgacagc atcgcgtccg cggccttggg atcgagcggc
     2041 cactgctgga tcaccttctg cagcgcgccg atggcgttcc tgtaatcgcg ccggcccgag
     2101 tagcccatgc cgatccagta ctgcgcgttc ggcgccagct tgccgttcgg gtacttcacc
     2161 agcagctgcc cgagcaggtc gatcgccgcc gagtagttcc ccaccttgaa ctggttgagc
     2221 gcactctcgt aggcgcgcct ttcctcctcg gagggcccgg cctcgggcgg cggcctcgcc
     2281 gcgacggccg cctgctgctc gatcgcgctc tcgagcttgc gcagccgcgt gtcgatgtcg
     2341 acgtagaggt ccttctgccg cttctcggcg acctccatct ggtagatcag cacctcgatc
     2401 tggccgcgct gcttggcgag atccgcgcgc agcgcctcga gctggccggc gaggtcgagg
     2461 atcgcgcgcc gctcgatcga ggtcgcttcc agcgccttca tgcgctcgcc gagccgctgc
     2521 tccatctgct gcagctgcag cagcgtctcg gtgcgcactt cctcgatgcg ccggcgcgcc
     2581 tggtcgtcgc cgccgaacag ctgcgcctgg gcggtgccgg ccagcgccac gagcagcgcc
     2641 gcggcgacgc cacgcacggc gttcatgtca gtactcgccc gtgtacagca tgtcgctgcg
     2701 gcggttgcgc gtccaggcct cctcggtgtg gccgagctcc ttgggctttt cctcgcccag
     2761 gctgacggcc tcgacctgct cctcgcgcgc gcccaggagc agcagcatgc gcttcacgcc
     2821 ctcggagcgg cgctggccga gcgcgatgtt gtactcgcgg ctgccgcgct cgtcggtgtt
     2881 gccctgaacc agcatcttca gctcgggctg cccgcgcagg taggccgcgt gcgcctcgat
     2941 cagcggcttg aactcgtcct tgatgtcgta gcggtcgaag tcgtagtaca ccgagcgcgc
     3001 cgccaggacg cttgccgggt cgcggtgcgg cgggacaccc ggcgtgggtg cgggcttgcc
     3061 gcccgtcagg tcgacgccgg tgaccggctt cgagggcacg ccctgcgtgc cggggccggg
     3121 cgccggctgc acggcgccgg gcttgcggtc ctccacgccc gccggcggct gcgtgccggg
     3181 cgtttccgtg gtcgcgcacg ccgcgagcgt cgcggcgatg aggatgctca gaagtattcg
     3241 catgatcgac ctccttctca tggatttagg ggtccccagg ccggctcgcg cacgtcgccg
     3301 gccgtgaccg agagtcgctg cttgacgcgc ccgtcggcgg aaaccgccgt gagcacgccg
     3361 cgccccgcta cgatggtcgc cagcaggatc aggcggccgt tgggggcgaa gctgggcgac
     3421 tcgtcgaggt cgctgtcggt gaggatctgg gtctggcgcg tggcgagctc gagcagtgcc
     3481 aactggaacc tgccgccgtt gcgcgcgatg taagcaaggc tgctaccgtc cgggttgacg
     3541 cggggcgaaa cgttgtagcc gccctcgaac gtgacgcgct gcgcgtcgcc gccgcgcagc
     3601 gccatgcggt agatctgcgg gctgccgccg cggtccgagg tgaagtagag ccagtcgccg
     3661 tcgggcgaga agaacggctc ggtatcgatg ccggccgagc gcgtgaagcg ctgcaggttg
     3721 ctgccgtcgg ggtcgatcag gtagatctgc gagttgccgt cgcgcgtgag cgcgagcgcg
     3781 aggcgcttgc cgtcgggcga ccaggccggc gccgagttcg agcccttgaa attggccacg
     3841 attcgttgct tgggcacctc gacgtcctgc acgtagacga tcggcttctt gttctggaac
     3901 gagacgtagg cgaggcgctt gccgtcgggc gaccaggccg gcgagatgat cggctcgttc
     3961 gagaccagca tcgcctgctc gttggcgccg tcggcgtccg ccacctgcag ctcgtagcgc
     4021 tcgccgcgct tcaccacgta cgcgatgcgc gtggaaaaca cgcccttctc gccggtgagc
     4081 ttctcgtaca cgtagtcggc gatgcggtgc gcggtggcgc gcacctgctc gcgcgtcagc
     4141 gtgtaagcga cgccgccgag cgccgtctgc ttcaccacgt cgtagaggcg aaagcgcacc
     4201 tcgtagcgcc catcggggcg cgcggccacc gagccgagca ccagcgcgtc ggcgagccgc
     4261 gcgcgccact cggcgtagtt cacgacggac gcctcggtcg gctgcggcac gaggcgcggg
     4321 atttccaggc tgcggaacag cccgctccgc tccaggtcgg cgcgcacgat ggcgccgatg
     4381 gtggcgcccg aggggtcgag cagcgcgccc tcgccggcga gcggcgcgat cgcgatcggg
     4441 atgcgctgcg cgccggcgcc ggtgatctcg atcgagagct gcgcgtgggc cgagcacgcg
     4501 gcgaaggcga agaggactgc cgcgagcagg tttcgcatcg gcctgattat atttcagtcc
     4561 ttcgggtgga acgtgagttt gaggtcgcgc tcgaacaggc ccggggtgtc gggcttcggc
     4621 aacggcgagg acttgaggat cgcgcgcatc accgcatcgt cgtacgcctg gtgcccgctc
     4681 gagatcacga gcttcgcctc cagcacctcg cccgtcggca gctgcgtcac cagcaccagc
     4741 gcctcgggat tgccctggat gtcgggcggc agcacgatgt tgccgcggat cttgccgcgg
     4801 atcttgtcga tccacgtctg catggcgcga ttgcgcgccg cctgctgctc gcgcagcagc
     4861 tggtcgcgga tctgctggcg ctcgcgatcg gcggcgcggc gctgatcctc gcgctgcagc
     4921 gcctcggcga gcaggcgctg cgccatggtc tcgtcgggcg gcggcgcggg ctcgggcttg
     4981 gccaccggct tgggctcggg ccggggctcc ggtttcggct cgggcctggg cttgggcttg
     5041 ggcttcgcct tcagcgcgat gtcgggcttc tcgatgcgcg gctcgggctt cggcgccggc
     5101 ggcgggggcg gctcgacctt gggcggcggc ttgaccttgg gcggcggcgc gggctgcgcc
     5161 accggctgcg gcggcgcctg ccacagctcg accatcacca cgtcgggcgc gcggctctgc
     5221 cagtgcacgc cgagcatcag tgccgctacc agcagcacgt gcaccgccag cgccagcatc
     5281 gccgagggac cgaagaaatg ctcgcgcggc gcctcgaaca gcaagccgct cgccgccttc
     5341 gccgccatca cttcgccggc tgcaccagca ggccgatgcg ccggatctgc tgccgctgca
     5401 gctcgtccat cacgttgagc accgcctcgt acttcacgtc cttgtcggcg gcgatgagca
     5461 ccgggcggtc cgggctcttc gcctggcgct cgcgcaccgc gcgtgcgagc tcgccgcgcg
     5521 cgaggcgccg ctcctcggcc agggcccctt gcgcgtcgcg cacgcgcagc gtgacgctct
     5581 tgtcctcgcg gatcacgatc tccagcacgg tggcgggcgc gttggccgcc ttcgacaccg
     5641 agggcaggtc caccacgccc ggcgtgatga gcggcgcagt caccatgaag atgacgagca
     5701 gcaccagcat cacgtcgacg tacggcacga tgttgatctc gtgcatcggc cggcgcgacc
     5761 gttgcggcgc gggcatcgct acttcaccgc ttggcggtga aggatgttgg agaactcttc
     5821 catgaagctc tcgaagcgga tcgagaggcg gtcgatcagg tacgagtagc ggttgtacgc
     5881 gaccaccgcg ggaatggcgg cgaagagccc cagcgccgtg gccaccagcg cctcggcgat
     5941 gccgggcgcg acctgcgcga gcgtcgcctg ctgcacgttg gccaggctcc tgaacgcatg
     6001 catgatgccc cacaccgtgc cgaccaggcc cacgtacggg ctcaccgagc ccgtggaggc
     6061 gagaaacgcg agatggcgct cgaggtggtc catctcgcgc tgggaggtgg cgcgcatggc
     6121 gcggcgcgcg ccgtccacca tgtcgctcgc gtcgccgctc gggcgctggt tgcgcagctt
     6181 cacgaactcg cgaaagccgg actcgaagat gcgctcgagg ctgccgatgg agtggcggtt
     6241 gttcaccgcg ccctggtaca gcaggttgag gtcgttgccg ccccagaact cgcgctcgaa
     6301 gcgctcggtc tgcgagcgcg cgcggcggat cgtgaaccac ttgctgaaga tgtagagcca
     6361 cgacatgaag gagaccacga ccagcagcgc catgaccgcc ttgaccaccg ggctcgcgcc
     6421 catgatcagg atccagatgt cgaggtcggt agtgacgttc atgggtttaa ccgcctagtt
     6481 gcgcggcgag cgccgcgggg atgggggtgg gcttgaaatc gtcgcggcgc acgcacgcga
     6541 cttggacccg gccttcggcc atcggttcca cgccgcacag cgcgcgctgg cgcagccaca
     6601 ggtaagtgcg tttccgctcg gtgcattcca cggtgatgct cagctcgtcg tccagtcgcg
     6661 cgccgcgcag gaaatcgatc tcggcccgcc gcaccacgaa ctgcagcccg tcctcgcgcg
     6721 ccaggcgcag gtggtcgacg ccgaggctgc gcagccactc ggtgcgcgcg cgctcgaaaa
     6781 agcgcaggta gttcgcatag tagacgactc cgccgaaatc ggtgtcttcg tagtagaccc
     6841 gcagcggcca gctgaacact tacgcgagca agtcgtgccc gcccgacggc gcggcgaggc
     6901 cgaagtggcg gtaggcggcg agcgtggcga cgcgcccgcg cggcgtgcgc tgcagatagc
     6961 cctgctggat gagatacggc tcgagcacgt cctcgatggt gtcggcgtcc tcgccgatgg
     7021 cgtgcgcgag gttctccacg cccacgggcc cgccgccgaa cttctcgatc acggcaagca
     7081 ggagcttgcg gtccatcacg tccaggccga gcacgtccac gtcgagcatg ccgagcgcgg
     7141 cctcggcgac gctcttcgac actgcgcccg cggccttcac ctgcgcatag tcgcgcacgc
     7201 ggcgcagcag ccggttcgcc acgcgcggcg tgccgcgcgc gcggcgcgcg atctcgacgc
     7261 cgccctcttc ggccagcgcc acttcgagca ggcccgccga gcggcgcacg atcgcggcga
     7321 gctcggtctc gttgtagaac tcgaggcgcg cgacgatgcc gaagcggtcg cgcaaggggt
     7381 tggtgagcat gccggcgcgc gtggtcgcgc ccaccagcgt gaagggcggc aggtcgagct
     7441 tgatcgagcg cgccgccggc ccctcgccga tcatgatgtc gatctggtag tcctcgagcg
     7501 ccgggtacag gatctcctcc accaccggcg acaggcggtg gatctcgtcg atgaacagca
     7561 cgtcgcgcgg ctccaggttg gtgagcagcg cggcaaggtc gcccgggcgc tcgagcaccg
     7621 ggcccgaggt ctggcgcagg ttgaccccca tctcgcgcgc gatgatgtgc gcgagcgtgg
     7681 tcttgcccag ccccggcggg ccgaacagca gcacgtggtc gagcgcctcg ttcctgccgc
     7741 gcgcggcctc gatgaagatg gcgagctggt cgcgcgtctt cgcctgcccg acgtagtcgg
     7801 cgagccgcgt cgggcgcagc gaacgctcga actgctcttc ctggggggag gcgggggccg
     7861 ccgcgatcaa gcgatcgctc tcgatggcca tgacgtgatt ttgccagacc cgggccgttc
     7921 accccttgtc gattgtgcgg cgcaggcata gcatttcggt gatgtccgcc gcccgcgcgc
     7981 tcgccgccgc cttcgccgcc ctctgggcct cgggtgtgct cgcgcaagcg aaggcgccgg
     8041 acaacggctg gtacctcggc ggcggcatcc gcccggtgat gccggcgatc gaggatagcg
     8101 acatcggtta ccggcatttc ggcggctacc gcttctcccg tcactggggc gtcgagcttg
     8161 gctactccga cctcgggcgc gggcccgaga gcaccgagcc ggcgttcctc gcccagcaga
     8221 agaccggcgc ccagacgagc gcctggacgc tggccgggac cggcgtgctg ccgatcggcg
     8281 aagccttctc cgtgcagggc cgtctcggct tctcggtgac ttcgcccgat gccacgttct
     8341 tggcgccctc gaccggaatc agctctgctt ttccgcgtta ccggcccacg gtgctctggg
     8401 gcttcggcgg tcagtacgac gtgaccggca gcatcggcct gcgcctcgat tacaacaatt
     8461 tcggtcggct ctcggacgac ccgaacggca cgcgctccga cctctggtcg atcaacgcgg
     8521 tagtgcgctt ttagccctca gggcttggcg agcgtcttga gcgcggcgcg gatgccttcc
     8581 gcgacgctca gcccgggggc caggccccgg gtcgccgcga gcgcctcctt gtcgttgtag
     8641 ccgagcgcga gcagcgcgtt cagcacgtcc gcgccggcgg cagtggtgcc cgccggcctc
     8701 gcctccgcga gcttgccctt cagctccagc agcagccgct cggcggtctt cttgccaatt
     8761 cctgggatct tcgtcaggcg cgcggcgtcc tgcatggcga cggcctgcga tagctcggcg
     8821 accgacagcc ccgagagcac ggagagcgcg gtgcgtgcgc ccacacccgc gatgcggatg
     8881 agctggcgga aagcgctgcg ctcctccagc gtggagaagc cgtagagcgc ctgcgcgtcc
     8941 tcgcgcacgg cgaggtgggt gtgcagggtg accggctcgc ccgtagccgg caggctgtag
     9001 aacgtgctca tcggcacgtc cacctcgtag gcgacgccgt tacagtcgac catcacctga
     9061 ggcgggtgct tggcgacgag cttcccagtg agccggccga tcacgccagc ctcccgcgcc
     9121 gcatgcgcag cccgcgcgtc gacaggcgcc cgaggcgcgc gccgtgcgcg tggcagatgg
     9181 cgcaggcgag cgcgtcggcg gcgtccgggc tcgggtcgcc cgcgagcgcg agcagccgct
     9241 tcaccatgtg ctggacctgc gactttcccg cctggccgtt gccgaccacc gattgcttga
     9301 cctgcagcgc cgtgtactcg gcgaccggca gcccggcgag caccgcggcg cagatcgcgg
     9361 cgccgcgcgc ctggccgagc gcgagcgtcg actggggatt gacgttgacg aacacctttt
     9421 ccaccaccac ctgctgcggc cggtgcgccg cgatcacctc ggcgaggccc tcgagcagcg
     9481 tcttcaggcg ctcggcgagc tcgccgcgcc ccgatttcac gcagccgctc gcgacatagg
     9541 cgaggcgctg gccgttcttg tcgacgacac cgaagcccgt gacctgcaag ccgggatcga
     9601 tgcccaggat cctcacgcgt cctcgaacac cgcggtggtg tagacgtcct gcacgtcgtc
     9661 cagcgattcg agcgcatcga gcaggctctt catgcgctgc gcgtcgctgc cggcgagcgc
     9721 ggcttcggtg gtcggcttga gcgtcacctc ggcgagctcg ggcttgagcc ctgccttgtc
     9781 gaacgccgcc ttcacggcga ggaactcgtg cggcgggcac acgacctcga tcgagccgtc
     9841 gtcgttggcc tgcacgtcct cggcgccggc ctccagcgcc gcgtccatga tcttctcttc
     9901 gctcgcgccg ggcgcgaaca cgaactgccc gcagtgcttg aacaggaacg cgaccgagcc
     9961 gtccgagccg aggttgccgc cgttcttggt gaacgcgtgg cgcacttcgg ccacggtgcg
    10021 ggtgcggttg tcggtgaggc aggcggcaat taccgccgcg ccgcccggac cgtagccctc
    10081 atagcgcact tcctcgtacg acacgccctc cagctgtccg gagccgcgct tgacggcgtt
    10141 cagcacgctg tccttggcca tgttcgcctc gcgcgccttg tccatggcga gccgcagccg
    10201 cgggttggtg gcggcatcgc ccccgcccag tttcgcggcg acggtgatct ccttgatcag
    10261 gcgcgtgaac accttgccct tcttcgcatc ggccgcggcc ttgcggtgct tgatgttcgc
    10321 ccatttgctg tgaccggcca tgcgataatt ttaccgtcca tgcccgctcc gctcctcgtc
    10381 gcgaagaacg ccaaggcgga gctccacctc ctgccggcgc tggccaaccg gcacgggctc
    10441 gtcaccggcg ccaccggcac cggcaagacc gtcaccctgc agaccctcgc cgagcagttc
    10501 tcgcgcgcgg gcgtgcccgt gttcatggcc gacgtgaagg gcgacctctc gggcatggcg
    10561 aaggcgggcg gcggcaacgc caaggtcgcc gagcgcgcca agctcctcga gctcgagctg
    10621 cggcacgagg cctgcccggt cgtgttctgg gacgtgttcg gcaagcgcgg ccacccggtg
    10681 cgcgccacga tttcggacat ggggccgctg ctcctttccc gcatgctcgg gctgaacgac
    10741 acgcaggaag gcgtgctcaa cctcgtcttc aggatcgccg acgaggcggg gctgctgctc
    10801 ctcgacgtga aggacctgcg cgccatgctg cagcacgtgg gcgagaacgc agcgcagttc
    10861 acgacccatt acggcaatgt ctccgcggcc tcgatcggcg ccattcagcg cggactgctc
    10921 acgctcgaga accagggcgc ggcgcagttc ttcggcgagc cgatgctgaa catcgccgac
    10981 ctgatgcagg tcgagagtgg caagggggcg gtcaacatcc tcgccgccga ccagctgatg
    11041 aactcgccca agctctacgg cacgtttctc ttgtggatgc tggccgagct cttcgagcaa
    11101 ctgcccgagg tgggcgaccc ggacaagccg aagatcgtgt tcttcttcga cgaggcgcac
    11161 ctgcttttcg aagacgcgcc caaagcgctg ctcgacaaga tcgagcaggt ggtgcggctc
    11221 atccgctcca agggcgtggg cgtgtacttc gtgacccaga accctctcga catccccgaa
    11281 tcggtgctcg ggcagctcgg caaccgcgtg cagcacgccc ttcgcgcctt cacgccgaag
    11341 gaccagaagg cggtgaaatc cgccgccgag accatgcggc ccaacccgaa gctgaagatc
    11401 gccgaggcga tcaccgagct cggcgtgggc gaagcgctgg tgagcctgct cgacgagaag
    11461 ggccggcccg gcatcaccga gcgcgccttc gtggtgccgc cgggcagcca gctcggcccg
    11521 ctcgccgagg ccgagcgcgc ggccgcgatc agcgcctcgc ccatcgccgg ccactacgag
    11581 aagacggtgg accgcgaatc ggcgtacgag aagctcaagg cgcgcgccga ccaggtggcg
    11641 caggagcagg ccgccgccaa gccgaaggaa tcgagcatgg cctccgacat cctgttcggc
    11701 aagaccggcc cgcgcggcgg ccgccagtcg cagggcctga tcgaggcgat ggcgaaaagc
    11761 gcggcccgca ccgtaggctc gcaggtggga cgcgagctca tccgcggcgt gctcgggtcg
    11821 ctcctcggca agcgtcgttg agtccggccg cgtaccgcgg ctggggcatg ctgttcgcgc
    11881 tcggcggcac cgtcgccttc gccttccgcc cggtgctgat caagctgggc tacgaggccg
    11941 cgccggtgag cgcgacgacg ctccttttcc tgcgcatgac gctgtcgctg ccgttcttcg
    12001 ccgcggtggc gtggtggcta aggcacgagc cacggctcgc gcggcgcgat tgggcgggca
    12061 tcgtcggcct ggggttcctc ggctactacc tcgcgagcct gctcgacttc ctcggcctgc
    12121 agtacgtgcc ggcgggcctg gggcggctga tcatgttcct gtatccgacg ctggtcgtga
    12181 tcctctcggc gctgttcctc gcgcggcgcc cgacggggcg cgagatcgcg gcgctcgcga
    12241 tgacctacgc cggcattgcg ctcgtgctcg cgcgccagct cggcgtcgag cccgagcatc
    12301 gcctcttcct gtggggcgcg gcgctcatct tcggctcggc gatgtcctac gcggtgtacc
    12361 tcgtcaccgg cagccagctc gtgaagcgcg tcggctcgat gcgcttcacc gcctacacga
    12421 tgatggtgtc gacggtgccc gcgctcgtgc agttcgcgct gctcgagcgc cccgccgcgc
    12481 tgcagctgcc gggcgaggtg tggctgtacg cgatcctgct cgccacggtg tgcacggtgc
    12541 tccccgtgtt tctcgtcgcc gaggcgctga agcggatcgg cgcgaaccac ttcgcgctca
    12601 tcggcgggct cgggcccgtc accacggtgc tcgccgattt cgcgctgctc gaaggcgcgc
    12661 tcgggccgct gcagctcctc ggcggagcgc tggtgattgc cggtgtattg ttcgtatccc
    12721 tcaagaaagg ctgaacacca tggacctcgg catcaaaggc aagaacgcgc tcgtgtgcgc
    12781 ggcatcgaag ggactgggca agggctgcgc catggcgctc gcgcgcgaag gcgtgaacct
    12841 cgtcatcacc gcgcgcggca aggaggcgct ggaggccacg gcggaggaga tccggaagag
    12901 caccggcgcc aaggtcacgt cggtcgccgg tgacatcact accgaggagg gccgcaaggc
    12961 cgccctcgcc gcttgcccga acgtcgacat cctggtgaac aacgcgggcg ggccgccgcc
    13021 gggggacttc cgcaactggt cgcgcgagga ctggatcaag gcggtggacg cgaacatgct
    13081 cacgccgatc gagctcatca aggcgacgat cgacggcatg atctcgagga agttcggccg
    13141 catcatcaac atcacctcgg gcgcggtgaa gatgccgatc gccgagctgg ggttgtccaa
    13201 cggcgcgcgt acggggctga ccggttttgt cgcggggctc tcgcgccagg tggcgcagca
    13261 caacgtgacc atcaacgggc tgctgcccgg gcctttcgac accgaccgcc tgcgcggcaa
    13321 catggagttc aacgcaaaga agctgggaac gacggccgcc gagctcgcca agcagcgcgc
    13381 cgccgccaac ccctcgaagc gcttcggcaa catcgacgag ttcggcgcga cctgcgcctt
    13441 cctcgccagc gtgcacgccg gctacatcac cggccagaac gtgctgatgg acggcggggc
    13501 gttcccgggg acgttctagc tagaacggat aaggcgtcgc cttgtcctgg atcgtcaccc
    13561 actgccactg ggtgaactcc tcccagttgg ccacgccgcc gtggcgcccg ccgttgcccg
    13621 aggcgccgcg cccgccgaag ggcacccagg gctcgtcggc cacggtctgg tcgttcacgt
    13681 ggatgatccc ggtcttcagc ttcgcggtga agttcatcgc gcgctcgacc gactgcgtga
    13741 tgacgccggt cgacaagccg tactcggtgg cgttgttgag cgcgatcgcc tcctcgtcgg
    13801 tgtcgaacgg cacgaccgcg agcacggggc cgaacacctc ctcggtgtac acccgcatcg
    13861 cgggcgtgac gttttcgagc acggtgggcg cgtaaaagag cttgtcgtag gtgccgcccg
    13921 cgcgcagcac cgcgcccttg gccacggcgt ccttcacgat gccgtcgatg cgctcgatct
    13981 ggcggccgtt gatgatcggc ccgagcgcgc acggtcccgc gctcggatcg cccaccggca
    14041 ggtgcttcgc tttggcgacg agccgctcgg tcaggtcgcg cgcgatcttc ctttgcgcga
    14101 gcaccgtgcc ggtcgccatg cagatctggc cctggtgcag gtaggcgccc
//
LOCUS       DBNHKE010000253         7565 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_1268508, whole genome shotgun sequence.
ACCESSION   DBNHKE010000253 DBNHKE010000000
VERSION     DBNHKE010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_1268508"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="AC16O9_17085"
     CDS             complement(<1..433)
                     /locus_tag="AC16O9_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518365.1"
                     /translation="MLIDFFLKLKSHKLPVSIKEYLTLLEAMGKGVIGPSVDDFYYLS
                     RTALVKDEANFDKFDRAFAEFWQGVETIPGIDAQVPLEWLLKQVELTLTEEEKKQIQA
                     LGGWEKLMETLKQRLEEQKGRHQGGSKWIGTGGTSPFGANGY"
     gene            640..2280
                     /locus_tag="AC16O9_17090"
     CDS             640..2280
                     /locus_tag="AC16O9_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6518366.1"
                     /translation="MHFDALIIGAGFAGIYQLHRLRDGLGLKARLLEAGGDLGGTWYW
                     NRYPGARCDSESHAYRYFFSRELAEEWVASERYPGPAEIRAYLEHVARKFDLRRDMQF
                     DTRVTAARYDEAAGRWEVDTEGGAHYSATYLVTAVGCLSAASVPEIPGLADFKGRWFH
                     TGQWPHEGVDFRGRRVGQIGTGSTGIQAAPVIAETAAHLTVFQRTANYSVPARNHPLT
                     REFQRWVKEHFEEIRDTARSTINGHAFRISERNAADASPEERAAVYRAAWEKGGLQFR
                     ATFRDLLTDRAANATAAEFIKARIRATVRDPATAEALAGIDHPYAAKRPPIDTGYFET
                     FNRPNVALVDLRKDPIERITPGGIRTRSREHPLDLIVFATGFDALTGPLLAIDIRGRG
                     GRSLREDWKAGPRTYLGLQVPGYPNLFTITGPGSPSVLTNMPVAIEQHVDWITECIAH
                     LRRNGIRTIEPAGEAARAWGEHVEECARATLLWDAKHSWYFGANVAGKPRVFMPYAGG
                     LARYARHCAGIAERGYEGFVLDRGRAVAAAAPVAQAGV"
     gene            2285..2722
                     /locus_tag="AC16O9_17095"
     CDS             2285..2722
                     /locus_tag="AC16O9_17095"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6518367.1"
                     /translation="MATALRALLERMTQAICRGDAEAAAACFAEGGVYHDGFYGEFQG
                     RAAIADMVRRHFHGNATDFSWTLSDVIAEGDRGYASYEFSYTSKIPGTEGRRAGFPGI
                     CKVRVKDGLIERYEEIFERAQALARLGFADERIVRSVRKWAAE"
     gene            3224..4180
                     /locus_tag="AC16O9_17100"
     CDS             3224..4180
                     /locus_tag="AC16O9_17100"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6518368.1"
                     /translation="MLEFLRGRSRLMQWISTTIVALVLGAPGWAAAQGEGGIAVFGTS
                     LSDPGNAFVLLGKAKSTPPDYSVDPFLVPDRPYARGGHHFSNGPTWVEQFARPRGLGA
                     GARPAFAPGGGTNFAVGSARARAVGANPHLAFQVGTFLTATGGVAPSDWLYVIEMGGN
                     DMRDALLAAASGMDPQPILNEASTAIYDNIEARYWAGARKFLVWNMPNVGLTPAIAPT
                     PLAGLANLLALQFNVKLEANVLTPLSSLEGIQIARFDVYTTLQNVAATPSSFGLTNVT
                     QACITLAAPYACAAPGDYLFWDGIHPTTAAHGILAFAVASAL"
     gene            complement(4206..4442)
                     /locus_tag="AC16O9_17105"
     CDS             complement(4206..4442)
                     /locus_tag="AC16O9_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518369.1"
                     /translation="MSEPITARIADHMRFARPLDTLAQEVARFHAADTPDYERLRHIV
                     LWLRDYADGVRHPREDVAFERLVRRDPSRAPGLA"
     gene            complement(4769..5422)
                     /locus_tag="AC16O9_17110"
     CDS             complement(4769..5422)
                     /locus_tag="AC16O9_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518370.1"
                     /translation="MTAGKISALIAVLALVGIVRANAFPLVPPMPQEHVVTITPGQNK
                     LLCFGGTTESSGYGGRCTPNPMDAKDSVTLDTTDGHVDGSHAGIYTNDSTMYGRKLGD
                     VSRLSFRYTGSSPTANSPRFSLPIDKDGDGAFDQWAFIHALDCNDGAGSVDAIINQKC
                     PIKLEGAATYDNWAALVAAFPNARIARNQYVLGLVFIVADEPGIWTVDRVKFGYPAK"
     gene            5850..7514
                     /locus_tag="AC16O9_17115"
     CDS             5850..7514
                     /locus_tag="AC16O9_17115"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6518371.1"
                     /translation="MGEIERYLAKPWLAHYQNGVPAAVEVPLVSVAQAFDQACDRDPG
                     RRAVVFYGRDVTYRELRDAVDRLANALAALGLKKGNRVALYLVNSPQFIIAYFAVLKC
                     GAVVTPISPVYTSHEVRHQLEDSGACAVICQDVLYEKVARSGVALDFVVVTQVGEYLP
                     AWKRLFGGKAIGIPSAPGIHWLQDLLKEHPPQPPAVTIDPKVDLAALPYTGGTTGNPK
                     GVMLTHYNLLAAQTSAQAAFPALEPGKEVLLAFLPFFHIYGQAVIMLNGLCQGNLLVL
                     FTNPDTDAILTAMERYQATVFYGVPTLYEYLKDHKDTGKVDWKRLKVVASGADTLHES
                     TMQGWAKRTGSNIIEGYGLSETSALSHVNPLHRTKPGSFGCPLPNMSAAVIDPETHAF
                     VPPGTTGELLLAGPNVMLGYWNQPEETARVFLEAGGVRWLRTGDIVRMDEEGYFHFYD
                     RSKDLIKFKGFSVFAKDVEDVLYAHPHVKAAGVVGVPEPSVGQLIKAIVVLQTDARGK
                     VSEDEIKTYCREQLAAYKVPHIVEFRGELPKTDVGKVSRRELREEH"
BASE COUNT         1263 a         2538 c         2493 g         1271 t
ORIGIN      
        1 tgtagccgtt ggcgccgaag ggcgaggtgc cgccggtgcc gatccacttc gagccgccct
       61 ggtggcggcc tttttgctcc tcgaggcgct gcttcagggt ctccatgagc ttttcccagc
      121 cgccgagcgc ctggatctgc ttcttctcct cttccgtgag cgtcagctcg acctgcttga
      181 gcagccattc gagcggaacc tgcgcgtcga taccggggat ggtctccacg ccctgccaga
      241 actccgcgaa ggcgcggtcg aacttgtcga aattcgcctc gtccttcacc agcgccgtgc
      301 ggctgaggta gtagaagtcg tccaccgacg ggccgatcac gcccttgccc atcgcctcga
      361 gcagcgtcag gtactccttg atggagaccg gcagcttgtg gctcttcagc ttgaggaaga
      421 agtcgatcag catggcgcgc gcccaggagg ccattgtagt ccgttgacaa cgggtgcaac
      481 ttcgcctccg ctccccatcg cgacatttgc cgctagaatc ggttcgattc gggcagcccg
      541 ggagcccctc ttgcacacgc gcagttcgcg cagccgcgtt ctcccggtcc acgcgcgtgg
      601 accgggcggc gccgcgtaag cggcggcgga aatagcgtca tgcacttcga cgccctgatc
      661 atcggcgcgg gttttgccgg gatctaccag ctgcaccgtc tgcgcgacgg cctcgggctc
      721 aaggcgcgcc tgctcgaggc gggcggggac ctcggcggca cctggtactg gaaccggtac
      781 ccgggcgcgc gctgcgattc ggagagccac gcgtaccgct atttcttctc gcgcgagctc
      841 gccgaggagt gggtggcgag cgagcgctac ccgggcccgg ccgagatccg cgcgtacctc
      901 gagcacgtgg cgcgcaagtt cgacctgcgg cgcgacatgc agttcgacac ccgcgtgact
      961 gccgcgcgct acgacgaggc tgccggccgc tgggaggtcg ataccgaagg cggcgcgcac
     1021 tattcggcga cgtacctcgt caccgcggtg ggctgcctgt cggcggccag cgtgccggag
     1081 atcccggggc tcgcggattt caagggccgc tggttccaca ccggccagtg gccgcacgag
     1141 ggcgtggact tccgcggccg gcgcgtcggc cagatcggca ccggctccac cggcatccag
     1201 gccgcgccgg tgatcgccga gaccgcggcg cacctcaccg tgttccagcg cacggccaac
     1261 tacagcgtgc cggcgcgcaa ccatccgctc acgcgcgagt tccagcgctg ggtcaaggag
     1321 cacttcgagg agatccgcga caccgcgcgc tcgaccatca acggccatgc cttccgcatc
     1381 tcggagcgca acgccgccga cgccagtccc gaggagcgcg cggcggtcta ccgggcggcc
     1441 tgggagaagg gcggcctgca gttccgcgcc accttccgcg acctgctcac cgaccgggcg
     1501 gccaacgcca ccgccgccga gttcatcaag gcgcgcatcc gcgcgaccgt cagggacccg
     1561 gcgaccgccg aagccctcgc cggcatcgac catccgtacg ccgccaagcg cccgccgatc
     1621 gacaccggct acttcgagac cttcaaccgg ccgaacgtcg cgctcgtcga cctgcgcaag
     1681 gacccgatcg agcgcatcac cccgggcggc atccggacgc ggagccgcga gcacccgctc
     1741 gacctcatcg tcttcgccac cggtttcgac gcgctcacgg ggccgctgct cgcaatcgac
     1801 atccgcggcc gcggcggccg cagcctgcgc gaggactgga aggcggggcc gcggacctac
     1861 ctcggcctgc aggtgccggg ctatccgaat ctcttcacga tcaccgggcc gggcagcccc
     1921 tcggtgctca ccaacatgcc ggtggcgatc gagcagcacg tggactggat caccgagtgc
     1981 atcgcgcacc tgcgcaggaa cggcatccgc accatcgagc ccgcgggcga ggcggcaaga
     2041 gcctggggcg agcacgtcga ggagtgcgcg cgggcgaccc tgctctggga cgcgaagcac
     2101 tcctggtact tcggcgccaa cgtcgccggg aagccccggg tcttcatgcc ctacgccgga
     2161 ggcctcgcgc gctatgcgcg ccactgcgcc ggcatcgccg agcgcggcta cgagggcttc
     2221 gtgctggacc gtgggcgtgc ggtcgcggcg gcggcgccgg tcgctcaggc gggcgtctag
     2281 ccccgtggcc acggcactgc gcgcgctcct cgagcgcatg acgcaggcga tctgccgcgg
     2341 cgacgcggag gccgcggcgg cctgcttcgc cgagggcggc gtctatcacg acggcttcta
     2401 cggcgagttc cagggccggg ccgcgatcgc ggacatggtg cggcggcact ttcacggcaa
     2461 cgccaccgat ttctcctgga cgctgagcga cgtcatcgcc gagggcgatc gcggctacgc
     2521 gagctacgag ttctcctaca cctcgaagat tccggggacc gaaggccgcc gtgcggggtt
     2581 tcccggaatc tgcaaggtgc gggtgaagga tggcctcatc gagcgctacg aggagatctt
     2641 cgagcgcgcg caggcgctcg cgcggctcgg gtttgccgac gagcgtatcg tgaggtcggt
     2701 gaggaagtgg gcggcggagt aaacccgaag gatgggaact tccttccgcg ttgtgccctg
     2761 taacacgcgc agcgacggac aacgctcgtt cggcgaggga gctgccatgg acctggcggc
     2821 gtatgagttc ggccgtaccg cgctcgcaca caggcgttca accgcgatta tggcaagtaa
     2881 gggcttcaga ccccccctcg ctccacgcag gtggtttcgt cgaggtcgag cgcggtcacc
     2941 ccaaggcgcg gccgctgcac gaggcagact cgcttggcgc catccgatgc tgaatctcct
     3001 tctccagccc attgagttcc tggggccatg gggtcgccct tttctcgtgc ggcattgatt
     3061 ctggtcaaga tttcgcctcc gaatcggggt agttagctag gttcggcagt cctcagcggg
     3121 acgccgccgg ttctcgccgc ggcgtgaccc ttggctgtcg tgcatccgct tggcagcaga
     3181 ggtcatgcca tgcgcttctt tgtcaacctg gaggacaaac gtcatgcttg aattcctacg
     3241 cgggcgcagc cgcctcatgc aatggatctc gacgacgatc gtcgcgctcg tgctcggcgc
     3301 gccaggctgg gcagcggcgc agggcgaggg cggcatcgca gtcttcggca ccagcctgtc
     3361 ggatcccggc aatgccttcg tgctgctggg caaagccaag agcacgccgc cggactactc
     3421 cgtggatccc ttcctcgttc ccgaccggcc ctatgcgcgc ggcggccatc acttcagcaa
     3481 tggcccgacg tgggtcgagc aattcgccag gccgcgcggg cttggcgcgg gcgcgcggcc
     3541 ggccttcgcg ccgggcggcg gcacgaactt cgccgtgggt agcgccagag cgcgcgctgt
     3601 tggcgcgaac ccgcacctgg cgttccaggt gggcaccttc cttacggcga cgggcggcgt
     3661 cgcgccctcg gactggctgt acgtgatcga gatgggcggc aacgacatgc gcgacgcgct
     3721 gctcgcggcc gcctccggca tggatcccca gcccatcctg aacgaggcgt cgacggcgat
     3781 ctatgacaac atcgaggcgc gttactgggc cggggcgcgg aagtttctcg tctggaacat
     3841 gcccaatgtc gggctcaccc cggcgatcgc cccgacgccg ctcgccgggc tggcgaacct
     3901 gctcgcgctg cagttcaacg tcaagctgga agcgaatgtc ctgacaccgc tctcgtcgct
     3961 ggaggggatc cagatcgcgc gcttcgacgt ctacaccacg ctgcagaacg tcgccgccac
     4021 gccgtcgagc ttcggcctca cgaacgtgac gcaggcctgc atcaccctgg ccgcgcccta
     4081 cgcctgcgcc gcgccggggg attacctgtt ctgggacggc atccatccca cgacggccgc
     4141 gcacggcatc ctggcgttcg cggtcgcaag cgcgctatag ccctcaggca agcagcgatg
     4201 cggggctagg cgaggcccgg cgcgcgcgag ggatcgcggc ggaccagccg ctcgaaggcg
     4261 acgtcctcgc gcgggtggcg cacgccgtcg gcgtagtcgc gcagccacag gacgatgtgg
     4321 cgcaaacgct cgtaatcggg ggtatccgcg gcatgaaacc gcgccacctc ctgcgccagc
     4381 gtatcgagcg gccgcgcgaa gcgcatatgg tcggcaatcc gcgccgtgat cggttcgctc
     4441 atgcccgcta tctgacaaag gaaatgacct tccttccgcg ttgcgccgag caacacgcgc
     4501 agtgtgggac agtggcaggt cggcgagggg gatcccgtgg ccctggcggc tcgtgcgtcc
     4561 ggccgtaccg cgctcgcaca caggcgttca accggcgtta tggcaaataa gatttaatac
     4621 tcaacggagt tcttcaacac aataggccaa ttgcggacgt tcctggtccc ccaaaaacca
     4681 aaaccccgcc taagcggggt tcgggtaaag ggctacgcgt gaaccagggg acctggtgcc
     4741 gtttcctacc tcgtgcgtga tggtgccttt acttcgcagg atagccaaac ttcacgcgat
     4801 cgaccgtcca gatgcccggt tcatcggcca ctatgaacac cagcccgagc acatactggt
     4861 tccgggcgat gcgggcattc ggaaatgccg cgaccagcgc tgcccagttg tcgtaggtcg
     4921 ctgcgccttc caacttgatc ggacacttct gattaatgat cgcatcgacc gagcccgcac
     4981 cgtcgttgca gtcgagcgca tgaatgaatg cccactggtc aaaggccccg tcaccgtcct
     5041 tgtcgatggg caggctgaac cggggactat tcgctgtagg cgaagacccg gtgtatctga
     5101 acgacagcct gctgacatcg cccagctttc gaccatacat cgtgctgtcg tttgtataga
     5161 tgcccgcgtg ggaaccgtcg acgtgtccgt ccgtggtatc gagcgtgacc gaatctttgg
     5221 catccatggg attcggcgtg cagcggccgc catatccaga gctttcggtc gtgccgccga
     5281 aacacagcag tttgttctgg ccaggcgtga tcgtgaccac gtgctcctgg ggcatcgggg
     5341 gtaccaacgg aaatgcattt gctctcacaa ttccaacaag cgccaaaacc gcgatcagcg
     5401 ccgaaatctt ccctgcagtc attgagtttt cctgggtgcg taaggtggac attgggaggg
     5461 caccgtacgc cctattggct ggaaagcaag gtaaatcaag cgcgggccga cggacggtac
     5521 tcgcctccgc cgcgattgac cgcttcgagt cgttagcggc caggttgggg aatgtccgct
     5581 aacgggctat cccgttgaaa atataggaac ttctttccgc gttgcgctgc acaactcgcg
     5641 cggctcaggg cggcgagggc gcgattcggg gatcgagggc cgaaggctcg agcggcgcac
     5701 aggcaaggcg ccgcggtagc ggcggggcaa ccggcgttaa ccggcgtgat ggtaaatgcg
     5761 agggtcttgc ctgggtcaag ccctggcttg caagctagat tcgcgcgaac ggtgacgcgc
     5821 gcaggctaga atcgcgccgg gaggaggcga tgggcgaaat cgagcggtat ctggcgaagc
     5881 cctggctcgc gcactaccag aacggcgtgc cggctgccgt cgaggtgccg ctcgtgtcgg
     5941 tcgcgcaggc gtttgaccag gcctgcgatc gcgaccccgg ccgccgcgcc gtcgtgttct
     6001 acggccgcga cgtcacctac cgcgagctgc gcgacgcggt cgaccgcctg gccaatgcgc
     6061 tggccgcgct cggcctgaag aagggcaacc gcgtggcgct gtatctggtc aacagcccgc
     6121 agttcatcat cgcctacttc gcggtgctca agtgcggcgc cgtggtgact ccgatcagcc
     6181 cggtctatac cagccacgaa gtccgccatc agctcgaaga cagcggcgcg tgcgcagtga
     6241 tctgccagga cgttctgtac gagaaggtcg cgagatcagg cgttgcgctc gatttcgtcg
     6301 tcgtgacgca ggtcggcgaa taccttccgg cgtggaagcg cctgttcggc ggcaaagcca
     6361 tcggcattcc ctccgcgccc ggcattcact ggctgcagga cctgctgaaa gaacatccgc
     6421 cgcaaccgcc cgcagtgacg atcgacccga aggtggacct tgccgcgttg ccctatacgg
     6481 gcggaaccac cggcaacccc aagggcgtca tgctcaccca ctacaacctg ctcgccgccc
     6541 aaaccagcgc gcaggcggcg tttccggctt tagagccggg caaggaagtg ctcctcgcgt
     6601 tcctgccgtt cttccacatc tacggccagg cggtgatcat gctgaacggc ctgtgccagg
     6661 gcaatctgct ggttctgttc accaaccccg acaccgatgc gattcttacg gccatggagc
     6721 gttaccaggc cactgtgttc tacggcgtgc ccacgctcta cgaatatctc aaggaccaca
     6781 aggacaccgg caaggtggac tggaagcggc tcaaggtggt ggcctcgggc gcggacacgc
     6841 tgcacgaatc gaccatgcag ggctgggcga aacgcaccgg ctccaacatc atcgagggct
     6901 acggtctgtc ggaaaccagc gccctgagtc acgtcaatcc gctgcaccga acgaagccgg
     6961 gatcgtttgg ctgcccgttg ccgaacatgt cggccgccgt catcgacccg gagacgcacg
     7021 catttgtgcc gcccggtacc acgggggaat tgctactcgc gggcccgaac gtgatgctcg
     7081 gttactggaa ccagcccgag gagacggcac gcgtgttcct cgaggcgggc ggcgtgcgct
     7141 ggttgcgcac cggcgacatc gtgcgcatgg acgaggaagg ctatttccat ttctatgacc
     7201 gctccaagga cctgatcaag ttcaagggct tctcggtttt cgccaaggac gtggaggacg
     7261 tgctctacgc ccatccgcac gtcaaggccg cgggcgtggt cggcgtgccg gaaccttcgg
     7321 tcggccagct catcaaggcc atcgtggtgc tgcagacgga tgcgcgcggc aaggtgagcg
     7381 aagacgagat caagacctat tgccgcgagc agctcgccgc gtacaaggtg ccgcacatcg
     7441 tggaattccg cggcgagttg cccaagaccg acgtgggcaa ggtctcgcgg cgcgagttgc
     7501 gcgaggagca ctgagcgcca tgaaccgccg cgttgcacgg ccatgaccga cgcgttcttc
     7561 tcggt
//
LOCUS       DBNHKE010000254         2144 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_686782, whole genome shotgun sequence.
ACCESSION   DBNHKE010000254 DBNHKE010000000
VERSION     DBNHKE010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_686782"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..451)
                     /locus_tag="AC16O9_17120"
     CDS             complement(137..451)
                     /locus_tag="AC16O9_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518372.1"
                     /translation="MIRMTKICIAIACAFGVGAALAQSVLRGDVKAGNAGDATQILAQ
                     ASTAAPTQTAPAGTAGGTSTGGMLAGVGTADKVGSTIVLVGAAVARTSAASDGSDTPP
                     SR"
     gene            679..1191
                     /locus_tag="AC16O9_17125"
     CDS             679..1191
                     /locus_tag="AC16O9_17125"
                     /inference="COORDINATES: protein motif:HMM:NF014639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR6518373.1"
                     /translation="MGIANLSLAKLSFRQIAAAGALTCAGLLGTGYYLQYFQGQDPCP
                     LCLLQRGFYYGILVVLAVGALHGPGRKGRIAYSIGALLFAAGGIATAVRHVWLQHLPP
                     DKVPACGPDLFYMMENFPLGRTLQLLLRGSGQCAEVHWRFLGLSIAEWSLLWFLALAA
                     LALTLALRKQ"
     gene            complement(1253..>2144)
                     /locus_tag="AC16O9_17130"
     CDS             complement(1253..>2144)
                     /locus_tag="AC16O9_17130"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6518374.1"
                     /translation="PPGYAHFISSDWQSPYRAQRIAELLRALPTHSLGSFARIQGDVV
                     SLPARELLPRLLEVKPASEEGRRALQLLTGWDASMSAERAEPLILWAWWREFSRAIYG
                     DELGEALRGNWLARAQFLTAVLTDRDGQGRWCDDVRTRSLESCDSVLAASLDTALADL
                     RQRYGGDMTEWRWGAAHEARHEHRPFGRVPWLARWFDIVVPSPGDAYTVNVGRNRMEN
                     EAHPFANSHAASMRAIYDLSDLDNSLYIHSGGQSGNILSAHYRSFSEAWSRVEYIPMI
                     ADRKKIEAAGAKRLRLAPGK"
BASE COUNT          321 a          744 c          697 g          382 t
ORIGIN      
        1 tttcattgcg gaggctcctt gaagatgctt ttccacactc tattcccctt ttcggcctag
       61 tggcaatcgg actcgccggc cccggaaagc aaaaggcccc ttgcggggcc ttcgcacttg
      121 ccttgcggtg ctgcgctcag cgcgagggag gcgtatcgct gccgtcgctc gcagccgagg
      181 tgcgcgccac cgccgcgccc accaaaacga tcgtgctgcc gaccttgtct gccgtaccga
      241 cgcccgcgag catgccgccc gtggacgtgc cgcccgccgt gccggccggc gcggtctggg
      301 tcggcgccgc cgtggacgcc tgtgccagta tctgcgtcgc gtcgcccgcg ttacccgcct
      361 tcacgtcgcc acgcagcacc gactgcgcca gcgccgcgcc cacgccgaat gcacacgcga
      421 ttgcaataca gatcttcgtc atgcggatca tgtgcaaccc cctacgccaa ttaactgcct
      481 agtcgcacgc cgcccttttc agaacttgtg ctgcctgcat gggcaaacag gcaatttgtc
      541 acaccttatg cccttgcagg ccgtcggcgc aatcccacga acgtgggagg gaaaggggtc
      601 acgcgcgtcc gccccgccgc gcttttccga aaagcttcac aaagtactgc gcatttccat
      661 ggtaacttgc tatgacatat ggggattgcg aatctatcgc tggccaagct atcttttcgt
      721 cagatagcag ctgcaggcgc gctgacctgc gcgggcctgc tcggcaccgg gtattacctg
      781 cagtacttcc agggccagga tccctgtccg ctgtgcctcc tgcagcgcgg cttctattac
      841 gggatcctcg tcgtgctggc ggttggcgcg ctgcacgggc ccgggcgcaa ggggaggatc
      901 gcgtattcga tcggcgcgct gctcttcgcg gcgggcggca tcgccacggc ggtgcgccac
      961 gtgtggctgc agcatctgcc gcccgacaag gtgccggctt gcggcccgga cctgttctac
     1021 atgatggaga acttcccgct cgggcgcacg ctgcagctgc tgctgcgcgg ctcgggccag
     1081 tgcgccgaag tgcactggcg ctttcttggc ctgtcgatcg ccgagtggtc gctcctctgg
     1141 ttcctcgccc tcgccgccct cgccctcacc ctcgcgctgc gcaagcaata aatagggaca
     1201 gacctcattt atcttttcgc cgcgtagcgg acaagataaa tgaggtctgt ccctatttac
     1261 cgggcgcgag gcgcaggcgt tttgcgcccg cggcctcgat cttcttgcgg tcggcgatca
     1321 tcgggatgta ctcgacgcgc gaccaggctt cgctgaacga cctgtagtgc gccgagagga
     1381 tgttgcccga ctgcccgccg gagtggatgt agagcgagtt gtcgaggtcg gaaaggtcgt
     1441 agatcgcgcg catgctcgcc gcgtgcgagt tggcgaacgg gtgcgcctcg ttctccatgc
     1501 ggttgcgccc cacgttcacc gtgtacgcgt cgccgggcga aggcacgacg atgtcgaacc
     1561 agcgcgcaag ccacggcacg cgcccgaacg gccggtgctc gtggcgcgcc tcgtgcgccg
     1621 cgccccagcg ccactccgtc atgtcgccgc cgtagcgctg gcgcagatcg gcgagcgccg
     1681 tatcgagcga tgccgcgagc accgagtcgc aactctccag ggagcgcgtg cgcacgtcgt
     1741 cgcaccagcg cccctggccg tcgcggtcgg tgagcacggc ggtgaggaac tgggcgcgcg
     1801 cgagccagtt gccgcgcagc gcctcgccga gctcgtcgcc gtagatcgcg cgcgagaact
     1861 cgcgccacca cgcccacagg atgagcggct cggcgcgctc ggcgctcatc gacgcatccc
     1921 atccggtaag aagctgcagc gcgcgccggc cctcttcgct tgccggcttc acttcgagca
     1981 ggcgcggcag gagctcgcgc gccggcagcg acaccacatc gccctggatg cgcgcgaagc
     2041 tgccgaggga gtgcgtggga agcgcgcgca gcagctcggc gatgcgctgc gcgcggtagg
     2101 gcgactgcca gtcgctggag atgaagtgcg cgtagcccgg cggc
//
LOCUS       DBNHKE010000255        19075 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_528622, whole genome shotgun sequence.
ACCESSION   DBNHKE010000255 DBNHKE010000000
VERSION     DBNHKE010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 19075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_528622"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="AC16O9_17135"
     CDS             complement(<1..930)
                     /locus_tag="AC16O9_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518375.1"
                     /translation="MSRTLDKLKQAERTTELRDRIATERGKEQTQQAKMAEDKRGLDL
                     ARERQQAEAELRRIAHARAEAETKAARLVADRERAEAHALQEAKAREQAERRATELAE
                     ARRRQEQAAAQHARERAESEQQAGQLMRERLAAEEQARREVAARADAERKAHEAARER
                     EATEKKLNRELEMRSAAEAEAKRLAEERAAIESAAVEQAHRNAEAERNATALAKERAK
                     RETEAAQAAAARAEASEAARAAAAERAAADKRATLATRERETRDTQAAHKARERAQAE
                     QDAARLAEERREGEERALAQAREREQNERRAAAA"
     gene            complement(953..2008)
                     /locus_tag="AC16O9_17140"
     CDS             complement(953..2008)
                     /locus_tag="AC16O9_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6518376.1"
                     /translation="MTYVAKPRLHHPTLPKNKLGFVRRDYEGKVSTLCAGCGHDSISA
                     ALIQAFWELDVEPHRVAKLSGIGCSSKTPDYFLGNSHGFNTVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHVMRRGVNMTYILENNGVYGLTKGQFSATADKGSKA
                     KKGMVNADAPVDTVSLALLMGATFVARSFSGDKKQLTPLIKAAIAHEGAAFIDVISPC
                     VAFNNHPGSTKSYDYVREHNEAVNRLDFWPSRDAITVDYSEGGVIEVPQHDGSIIKLR
                     KLDAEYDPSDKINAVSHIARAAEKGEVLTGLLYLAPDAKDLHAHLNTYEVPLNRLSEK
                     ELCPGAGMLEKINAAMR"
     gene            complement(2005..3855)
                     /locus_tag="AC16O9_17145"
     CDS             complement(2005..3855)
                     /locus_tag="AC16O9_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6518377.1"
                     /translation="MMSLKRIEALNDFVVKFANVNGSGSASANELFAKSFLRMGIPVS
                     PRNIFPSNIQGLPTWYEVRVTEKGWLGRRGGVDLMVAMNPQTWAQDVKEIEPGGYLFY
                     DNTKPLPPSKFRDDVNVIGMPLTEISNATFTDARERQLFKNIIYVGALSALMEIEPGV
                     IAELIGEQYKGKEKLLKPNLNAFQMGHNYAGEKLKGMLGLKVKRANAVGDKIFMEGNA
                     AAALGCVYGGATVCAWYPITPSSSLAEAFQRYCSRLRVDAQTKQNKFAIIQAEDELAS
                     IGIVTGAGWNGARAFTATSGPGISLMSEFIGLAYFAEIPAVIIDVQRGGPSTGMPTRT
                     QQSDVLACAYASHGDTKHVLLFPEDPRECFEFAAASLDLAERLQTPVFMMTDLDIGMN
                     QRLCEPLAWDDARRYDRGKVMTAEMLDEGKEFGRYLDVDGDGIPYRTYPGTHESKGAY
                     FTRGTSKNPYAVYSEAGSDYRYNMERLLKKHDFAKTLVPKPVLTASREPARFGVIYYG
                     STSPSMHEALDALTEKDIFVNALRVRAFPFQDEIADFVQSHSRVFVVEQNRDAQLKTL
                     LVNEAGVNPASLISVLHYDGTPITARFIAREIAEIVSALNVRPIKKDKAA"
     gene            complement(3852..5678)
                     /locus_tag="AC16O9_17150"
     CDS             complement(3852..5678)
                     /locus_tag="AC16O9_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6518378.1"
                     /translation="MQPTDIRNPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRHDDAF
                     MVNWKSNVFPGILGRTCDRPCEPACRRGRVEEENQARPEPVAICRLKRVAADNKDSAG
                     VKARMPRPAKQKNGKRIACVGAGPASLTVARDLAPLGYQVTVFDQDPRAGGMIWSQIP
                     RFRLPLEVIDEEVGYVLDLGVEFKGGHRIESMTKLMAEGFDAIFVGSGAPRGRDLDIP
                     GRKEAAKNIHIGIDWLSSVSFGHTTGIGKRVIVLGGGNTAMDCCRTSKRIGGEDVKVI
                     VRSGFEEMKASPWEKEDAQHEGIPILNYLVPKAFLHENGRLKGMTFEKVKAEYDDKGR
                     RKLVPTGEPDQTHACDDVLVAVGQENAFPWIERDSGIEFDKWGLPVLDEATFQSSVKN
                     VFFGGDAAFGPKNIIWAVAHGHGAAISIDKLLNGEDLRERPAPTVNLLSQKMGIHEWS
                     YDNEIAPDARFKVPWRDIKVTLKNVKTEVELGFDVATAWKEAQRCLNCDVQTVFQARQ
                     CIECDACVDICPMDCITFTQNGEEAELRQRLMAPATNLAQALYVQDGLKTGRVMVKDE
                     DMCLHCGLCAERCPTGAWDMQKFLLEMAAAGPGCRSRERKAA"
     gene            complement(5804..7294)
                     /locus_tag="AC16O9_17155"
     CDS             complement(5804..7294)
                     /locus_tag="AC16O9_17155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6518379.1"
                     /translation="MNSGIDWNELLALSADWYWEQDADMKFTVFTSAELEAAGMAASD
                     YLGRPRWETPHHGVSASQWDQHRALLERREPFRDFIVGRYRGDGSECFVAISGQPMLD
                     AGGRFIGYRGISRDVTARVIAERALRESEARYRALTDVSPAAIYVQVGGILRYVNPAA
                     LRLFGESRAADVVGRPLLEYIHGDERADVAERMRRVRDEGASYPPTVRNFLRRDGTLF
                     TGEVSVVPHVYQGEAGSIAVVRDITERQRAEAEIRTLNAELEARVRQRTAQLQRLNDE
                     LESFSYSVSHDLRAPLRAIHGFSDLLAQQAGAALDAQAGHYLERVQDAAARMGRLIDD
                     LLELSRVTRAPVRPEPVDLSRLALQVADELRAGEPRRAVEIAIAPGLAARGDPALLRV
                     VLQNLLENAWKFTRRREGARIEVGEQRAAGEHAFFVRDNGAGFDMAYAGKLFGAFQRL
                     HSEEHFPGTGIGLATVKRIVERHGGRAWAEGEPEHGAAFFFTLAEG"
     gene            7426..8385
                     /gene="tal"
                     /locus_tag="AC16O9_17160"
     CDS             7426..8385
                     /gene="tal"
                     /locus_tag="AC16O9_17160"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR6518380.1"
                     /translation="MPTQLESLRRHSTVVADTGDIDAIARWRPQDATTNPSLLLAAAQ
                     EARFRPLVDEALERGRGAPEPTLEWLFVLFGREILKHMPGRVSTEVDARLSFDAAGSI
                     AKARRFIELYDQAGVARERVLIKLASTWEGIRAAEKLEREGIRCNMTLLFSFAQAAAC
                     ADAGVALISPFVGRIYDWHKAARKVDDIPIAEDPGVASVTRIYQYYKKFGYATQVMGA
                     SFRKTGQILALAGCDLLTIAPDLLEKLAETEGEVARCLSPQDAAAQGFERVALDEKGY
                     RWRHNEDAMAVEKLAEGIRRFDADARKLEQFVLPLVKERRAAA"
     gene            8391..9164
                     /locus_tag="AC16O9_17165"
     CDS             8391..9164
                     /locus_tag="AC16O9_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6518381.1"
                     /translation="MAAPIQTTLEERAGGSVARVTLANPAKLNALDAQAILALAETMR
                     GLAGDLKLRAVVLTGAGDKAFSGGVDVAELGATTPATAREAITRLHRCITAVRECPVP
                     VIARVNGYCIGGALELAAGCDLRIAADHARFGMPEVRLGIPSVIEAALLARLMGAGRA
                     RWLLLTGELIDAAEALRWGLVERVVPAQGLDAEVDHALECILESGAEAIRAQKRLNRA
                     YEELPFAEAVAQSIDAFEQSYRTGEPARQIGDYLRRRRK"
     gene            complement(9140..9334)
                     /locus_tag="AC16O9_17170"
     CDS             complement(9140..9334)
                     /locus_tag="AC16O9_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518382.1"
                     /translation="MPLWKRFWLLGSAIWVVVCVLNAVTILAFSEGEGAKAAQPMVLA
                     VAGPALLYAVLWLYFRLRRK"
     gene            complement(9334..10005)
                     /locus_tag="AC16O9_17175"
     CDS             complement(9334..10005)
                     /locus_tag="AC16O9_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518383.1"
                     /translation="MRSSSRSQLLLGAFAGLIVLVIGGSFYYGSLKKQALESSAAASV
                     ADATARLRDAAALPLDAPKAAAQLQAHEAALGAHLERLRAEDASRNKSLAEAAELYLV
                     DVQAMLRVQANAARALAAAQGSRRALAAHMEHAEGRGAAWIQRALALKQKAERDNFDY
                     RTAVGTLAGLYYAHGESQQKLRAVVPEAPLLAEAERRRLHQAATEAEAKAVQDLEQLR
                     RLPIG"
     gene            10070..10885
                     /locus_tag="AC16O9_17180"
     CDS             10070..10885
                     /locus_tag="AC16O9_17180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6518384.1"
                     /translation="MLPELKSAADITYRESGRKEAPALVLLHGIGSTSAGWRLQYGPL
                     GAHFRVIAWDAPGYGGSKPLAADAPSAEDYARALARLLDALGVESAIIGTNSWGTPTG
                     VVFARLFPARVRALVLGGPAAGWGAAPQAEREQRIAARIERVSTLGVQRMRADDAAEL
                     VAPGTRAEVLAWIKGAEGLNASGYAQAARMLGAVDVPREVTAVACPVRVVSGTLDRRT
                     PPETNAKRIAAAAPNATLQMVPDCGHLPHLEHPEVFNAAVLAMARSLGEARAA"
     gene            10882..11961
                     /locus_tag="AC16O9_17185"
     CDS             10882..11961
                     /locus_tag="AC16O9_17185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR6518385.1"
                     /translation="MNAPQAHEFLTLHEIVAAARAALPPGPWAYLIGGTETETTVRRN
                     RQALDSLAFRPRVLRDVRRVAATASCLGRKVRLPVLLAPIGGLESLVEGGAAAAARGA
                     AQFGVPQMLSSVCKPGLEETAAAADALRIFQLYVRGDDAWVDDYVRRARDHGYAAFCL
                     TVDVAMYSRRERDLVGRFVKPWRADAAQGRDFQAALAWDQVKRYKDRHDLPLVLKGIA
                     TAEDAQIAVEHGVDVIYVSNHGGRQLDHGLGSIAVLPEVVAAVGGRAQVWVDGGFMRG
                     TDVVKALALGAQAVGLGRLACLGLAAAGTPGLVRALEILEEEIVTCLGLLGATALAEL
                     GPRHLASAAPVGPAGALSAFPLLGE"
     gene            12005..13756
                     /locus_tag="AC16O9_17190"
     CDS             12005..13756
                     /locus_tag="AC16O9_17190"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6518386.1"
                     /translation="MALKTLGRYRILGELGRGAMGVVYRAVDPVIEREVALKALHAEL
                     PADVAGEVRVRFLREARSAGRLNHPNIVTIFDVGEDAGAAYIAMELLDGPSLAQMLKE
                     RQTIAFHVAADIIAQVADALHHAHQHSIVHRDVKPANVVVSRTGRAKLTDFGVAYVPA
                     SDVTQHGSALGSPRYMAPEQVLGQPIDARADVFSLGVVLYELLTRRTPFEWPGDTTVF
                     ALMQRIAGEPHPPLRQIDAAIPAGFDRIMDRALAKRPQDRYQSAAELAADLRNYHSLG
                     GGTEFAKTVPQETLRPATVVAEEQSLRNQLLDDLEQFAKDFETQEVARLRAEQEARLR
                     KEQALQRWGAAQAHEREAFERGERVVPDEDPKSMTTTRRMEAVEIMRQQAAFRKAQAE
                     AKPKAMAALDAAMRAVLQYLTEFGKEMQAHAPHAGAPYEFIYVGKVPSVTLVQPTVSN
                     RTLKLPENKELCAQIQFRFWVQPAEPPKFMLLGEDVTRCEQYLKSLRVSFEKRARGDD
                     KERPVQFVVNGPLPCDVNVRADYEALTATLELINVRRFGRFTVRLDTASFKAAIDDLA
                     RYVLGVDDEFEKLIPEA"
     gene            13801..13974
                     /locus_tag="AC16O9_17195"
     CDS             13801..13974
                     /locus_tag="AC16O9_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518387.1"
                     /translation="MALAHGEPPQAGDADDDAEAEAAQKAASFDTAGDKAQANFRRRV
                     GEALQEMRGPRES"
     gene            complement(14009..15493)
                     /locus_tag="AC16O9_17200"
     CDS             complement(14009..15493)
                     /locus_tag="AC16O9_17200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6518388.1"
                     /translation="MDKKILLVTAPYQCWGVQVVGTWPPLHLVYLAGAAREAGVVPKI
                     HDAMNESHSYADIRAQIESFRPDYVLAYDYLPVTGAISTAALPASLAILKLAKEIDPG
                     IVTLLGGPHPTFMSKEVLESERGVVDYVLLGEGEATLKALLQALRGGSASGVKGIAYL
                     DGARAVGTGSRALIADLDQLQPAWDLLRWDAYRYRVSPGGRMASILSSRGCEMHCSFC
                     SQRLFWRGAWRARRPEKVVEEIVLLHQRYGVTVFTLIDAFPTRDRERWERLLDLLIER
                     DLGLSVLMETRVEDIVRDADILHKYRKAGVIHIYMGAESGDDAVLTSLNKGIEMKTTK
                     QALALLNQAGIISEASFMIGFPQETPSSVRNTIDAAIYLNPDVAVFPIVTPWPYTPMY
                     KEMKDRIRVFDYAKYNLVTPIIEPFEMSLEEVNEALANCYMEFYAHKLKEVLALEKGF
                     KRDYLMSAFRLMMKDQCSAFRMKGARMPHQATEMLGLIAEFRAA"
     gene            complement(15493..15885)
                     /locus_tag="AC16O9_17205"
     CDS             complement(15493..15885)
                     /locus_tag="AC16O9_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518389.1"
                     /translation="MSDSTLAILVMLNNLFHDFAVALLFASLLMLGLFYRCIRGQGDP
                     ADTALVRVVAGTFEKVVYACWAALILGGIGRTLAYESFEWNEAAGRGQVAALVVKHVL
                     LVAVVVWGTYIQHRLRKHLKRLKAQATN"
     gene            complement(15903..16934)
                     /locus_tag="AC16O9_17210"
     CDS             complement(15903..16934)
                     /locus_tag="AC16O9_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6518390.1"
                     /translation="MRWQLTRPQWTILAVLLVAALVGWSVAYFTRSPMERADAITEPT
                     AFDQPPIFVGMHGVAEAAALKPFALALNEKKLLVSYLSTDRVDEFSDRLEFRRTLHLR
                     QNEPASITGVAVEGDRIYATDFVSGDLLVAEYKTGKLLQSYGWLPGYQTRMKALGVAY
                     FQNNLYVSDVASGKMLAIGALAQKDVRDEGELIVAFPNGGPSEFELGYPTWSMVTADG
                     RLLVSDAKSGEVKAFTCSGRSAHLFDQAEPARLKTPMGIAMDDLRSPELLAKARRVFD
                     PSRVHELGRIHVVDAAQARVKVFDALGKYVLTYGEELRQPNGIAIDPKQRLIYISDVQ
                     LRAIALYKY"
     gene            complement(16938..17744)
                     /gene="ccsA"
                     /locus_tag="AC16O9_17215"
     CDS             complement(16938..17744)
                     /gene="ccsA"
                     /locus_tag="AC16O9_17215"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR6518391.1"
                     /translation="MFWLAAVVYAIAFGLFAVALVFRKPGTLGHAITVAWAGFFAHSV
                     VMVARWVQTGHPAFVSYFESMSASAWFGMLAFLALQQWKPFLRPAGAGLMPLIVLLMG
                     WAGTRPFGGQSLPVSLQSFWLFVHAGFATAAAGCFLLAAGVAGMRLYQQRRNAPSDPA
                     YHIAPGDRYDEFNFRLILLGFLFWGIMTVSGAIWADGAWGRYWAWDPVEVWSLISWLV
                     YAVYLHIYTVWRRLRGVFLAWYAIIGALFVAMSLWGIGFLYRTLHTYGGN"
     gene            complement(17768..18589)
                     /gene="ccsA"
                     /locus_tag="AC16O9_17220"
     CDS             complement(17768..18589)
                     /gene="ccsA"
                     /locus_tag="AC16O9_17220"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR6518392.1"
                     /translation="MTSLEAGLLFGAGALYASSFLLAGKGWLRYGFVSVVAGLALHLA
                     SAAIRWVGTGHPPVFGTFEATLAASWFLMLFVALAFGSVHRQFRLLVLTAVPIALLLL
                     AYGVTFFDTSRIPLTISERSLWVEFHALFSWLAFAPFTLAFCLSGSYLWSTRSRRAAA
                     PPPAHADILDELAFRYITLGFVAHSVMFALGSYYSSILFGTWWRWDPAFSLSLVAWLL
                     VGLYIHLRLFYHWNSKRAARSYLLIFAVIGFSYWGLIYLPAGRTFHVFDLDLKAL"
     gene            complement(18586..>19075)
                     /locus_tag="AC16O9_17225"
     CDS             complement(18586..>19075)
                     /locus_tag="AC16O9_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6518393.1"
                     /translation="VKLNIFPPGSEDYFHVPEYPHKIFVSFYPDHAQVDGKAVSKSMH
                     PVNPVYMLRIFRGRLPVFTGAIKPGEWADFDGLSISFPSYVKSGTFQIVSNPGHPIIW
                     AAFIVMGLGLAWRLLFYRKNIALWQDDAGRTWLSGRFDYFPRLNAGWLASLGEQFKAE
                     AA"
BASE COUNT         3005 a         6634 c         6467 g         2969 t
ORIGIN      
        1 cgccgccgcc gccctgcgct cgttctgctc gcgctcgcgc gcctgcgcga gcgcccgctc
       61 ctcgccctcg cggcgctcct cggcgaggcg tgccgcgtcc tgctcggcct gcgcgcgctc
      121 gcgcgccttg tgcgcggcct gcgtgtcccg ggtctcgcgc tcgcgcgtgg cgagcgtggc
      181 gcgcttgtcg gccgcggcgc gctcggccgc cgccgcgcgc gccgcctcgc tcgcttcggc
      241 ccgggccgcg gctgcctgcg cggcttccgt ttcgcgcttg gcgcgttctt ttgcgagcgc
      301 ggtggcattg cgctcggcct cggcgttgcg gtgcgcctgc tccaccgccg ccgactcgat
      361 cgccgcgcgc tcctcggcga ggcgtttcgc ctcggcttcc gccgcgctgc gcatctcgag
      421 ctcgcgattg agctttttct cggtcgcctc gcgctcgcgc gcggcttcgt gcgccttgcg
      481 ctcggcgtcg gcgcgggccg cgacttcgcg ccgcgcctgc tcctcggccg caaggcgctc
      541 gcgcatcagc tgcccggcct gctgctccga ctccgcgcgc tcgcgcgcat gctgcgcggc
      601 ggcttgttcc tgcctgcggc gcgcctcggc aagctcggtg gcacggcgct cggcctgctc
      661 ccgggccttg gcttcctgca gcgcgtgcgc ttccgcgcgc tcgcggtcgg cgaccaggcg
      721 cgccgccttg gtttcggcct cggcgcgggc gtgggcgatg cgccgcagct cggcttcggc
      781 ctgctggcgc tcgcgcgcga ggtccaggcc gcgcttatcc tcggccatct tcgcctgctg
      841 ggtctgctcc ttgccgcgct cggtggcgat gcggtcgcgc agctcggtcg tgcgctccgc
      901 ctgcttgagc ttgtcgaggg tgcggctcat gacaggcgga ggctaatcgc cctcagcgca
      961 ttgccgcgtt gatcttctcc agcatccccg ccccagggca caactctttt tcagacagcc
     1021 tattgagcgg cacctcatag gtattgaggt gtgcatgcag gtccttggca tccggcgcca
     1081 gatacagcag cccggtcaga acctctccct tctccgccgc ccgcgcgata tgggataccg
     1141 cgttgatctt gtccgaaggg tcgtactccg catccaattt gcgcagcttg atgatcgacc
     1201 catcatgctg cggcacctcg atcaccccgc cttccgaata atcgacggta atcgcatccc
     1261 gcgacggcca gaagtccaga cgattcacgg cctcgttgtg ctcccggacg tagtcgtagc
     1321 tcttggtcga gcccggatgg ttgttgaacg cgacgcaggg cgagatcacg tcgatgaaag
     1381 ccgctccctc atgcgcgatc gccgccttga tcaacggcgt gagctgcttc ttgtcgcctg
     1441 aaaaggaacg cgccacaaac gtggcgccca tcagcaacgc cagcgacacc gtgtccaccg
     1501 gcgcatcggc attcaccatg cccttcttcg ccttcgagcc cttgtcggcg gtcgccgaga
     1561 actggccctt ggtcagcccg tacaccccgt tgttctccag gatgtaggtc atgttcaccc
     1621 cgcgccgcat cacgtgcgcg aactgcccca ggccgatgga agccgaatcc ccgtcgccgg
     1681 acaccccgag atagatcaaa tcccgattgg ccaaattcgc cccggtcagc accgacggca
     1741 tgcgcccgtg cacggtgttg aacccgtggg aattccccaa aaagtaatcc ggcgtcttgg
     1801 aagaacaccc gatccccgaa agcttcgcca cccgatgcgg ctcgacatcg agctcccaga
     1861 acgcctggat cagcgccgcg gaaatcgagt cgtgcccgca gccggcgcac aaagtagaca
     1921 ccttgccttc gtaatcacga cgaacaaacc ccaacttgtt cttcggcagg gtcggatggt
     1981 gcagccgggg cttggcaacg taggtcatgc ggccttgtcc ttcttaatcg gccgcacgtt
     2041 cagcgcggac acgatttcgg cgatctccct ggcgatgaac cgcgccgtga tcggcgtgcc
     2101 gtcgtagtgc agcaccgaaa tcagcgaggc cggattgacg ccggcctcgt tgacgagcag
     2161 cgtcttgagc tgcgcatccc ggttctgctc caccacgaac accctggagt gcgactgcac
     2221 gaagtcggcg atctcgtcct ggaacggaaa cgcccgcacg cgcagcgcgt tgacgaagat
     2281 gtccttctcg gtcagcgcat cgagcgcctc gtgcatcgac ggcgaggtcg agccgtagta
     2341 gatcaccccg aagcgcgccg gctcgcgcga cgcggtcagc acgggcttgg gcaccagcgt
     2401 cttggcgaag tcgtgcttct tcagcagccg ctccatgttg tagcggtaat cggacccggc
     2461 ttccgaatac accgcgtacg ggttcttgct ggtgccgcgc gtgaagtagg cgcctttcga
     2521 ctcatgggtc cccgggtaag tccggtacgg tatgccgtcc ccgtccacgt ccagatagcg
     2581 cccgaactcc ttgccctcgt ccagcatctc ggcggtcatc accttgccgc ggtcgtaccg
     2641 gcgcgcgtcg tcccacgcga gcggctcgca cagccgctgg ttcatgccga tgtcgaggtc
     2701 ggtcatcatg aacaccggcg tctgcagccg ctcggccagg tcgagcgagg ccgccgcgaa
     2761 ctcgaagcac tcgcgcggat cctcgggaaa gagcagcacg tgcttggtgt cgccatgaga
     2821 cgcatatgca caggccagca cgtccgactg ctgggtgcgc gtcggcatgc cggtcgaagg
     2881 cccgccgcgc tgcacgtcga tgatcaccgc cgggatctcg gcgaaatagg ccagcccgat
     2941 gaactcgctc atcagcgaga tccccggtcc ggaagtcgcg gtaaacgccc gcgccccgtt
     3001 ccaccccgcc ccggtgacga tgccgatcga ggcgagctcg tcctcggcct ggatgatggc
     3061 gaacttgttc tgcttggtct gtgcatccac gcgcagccgc gagcagtagc gctggaacgc
     3121 ttcggccagg gacgaggacg gcgtgatcgg ataccaggcg cacaccgtgg cgccgccgta
     3181 cacgcagccc agcgcggccg cggcgttgcc ttccatgaag atcttgtcgc cgaccgcgtt
     3241 ggcgcgcttc accttgagcc cgagcatgcc cttcagcttc tcgccggcgt agttgtggcc
     3301 catctggaag gcgttcaggt tgggcttgag gagcttttcc ttgcccttgt actgctcgcc
     3361 gatcagctcg gcgatgacgc cgggctcgat ctccatcagc gcggacagcg cgccgacata
     3421 gatgatgttc ttgaacagct gccgctcgcg cgcgtcggtg aacgtggcgt tgctgatctc
     3481 ggtgagcggc atgccgatga cgttgacgtc gtcgcggaac ttcgacggcg gcaagggctt
     3541 ggtgttgtcg tagaacaggt acccgccggg ctcgatctcc ttcacgtcct gcgcccaggt
     3601 ctgcggattc atcgccacca tcaggtcgac gccgccacgc cggccgagcc agcctttctc
     3661 ggtgacgcgc acctcgtacc aggtgggcag gccctggatg ttggagggga agatgttgcg
     3721 cgggctgacc gggatgccca tccgcaggaa gctcttcgcg aacagctcgt tggcggaggc
     3781 cgagcccgag ccgttcacgt tggcgaactt gacgacgaag tcgttcagcg cctcgatgcg
     3841 cttcagggac atcatgcggc cttccgctcg cggctgcggc agccgggccc ggccgcggcc
     3901 atctcgagca ggaatttctg catgtcccag gcgccggtcg ggcagcgctc ggcgcacagg
     3961 ccgcagtgca ggcacatgtc ctcgtccttc accatcacgc gcccggtctt caggccgtcc
     4021 tgcacgtaga gcgcctgcgc caggttcgtc gccggcgcca tcaggcgctg gcgcagctcg
     4081 gcttcctcgc cgttctgcgt gaaggtgatg cagtccatcg ggcagatgtc gacgcaggcg
     4141 tcgcactcga tgcactgcct ggcctggaat accgtctgca cgtcgcagtt caggcagcgc
     4201 tgcgcttcct tccacgcggt ggcgacgtcg aaaccgagct cgacttcggt cttcacgttc
     4261 ttcagcgtca ccttgatgtc gcgccagggc accttgaagc gcgcgtcggg cgcgatctcg
     4321 ttgtcgtagc tccactcgtg gatgcccatc ttctgcgaca gcaggttcac ggtgggcgcc
     4381 gggcgctcgc ggaggtcctc gccgttgagc agcttgtcga tcgagatggc ggcgccgtgg
     4441 ccgtgcgcca ccgcccagat gatgttcttc ggcccgaacg ccgcgtcgcc gccgaagaag
     4501 acgttcttca ccgaggactg gaacgtggcc tcatcgagca ccggcaggcc ccacttgtcg
     4561 aactcgatgc cgctgtcgcg ctcgatccag ggaaaagcgt tctcctgccc caccgcgacg
     4621 agcacgtcgt cgcacgcgtg cgtctggtcg ggctcgcccg tgggcacgag cttgcgccgc
     4681 cccttgtcgt cgtactcggc cttcaccttc tcgaaggtca tgcccttcag cctgccgttc
     4741 tcgtgcagga aggccttggg cacgaggtag ttgaggatcg ggatgccctc gtgctgcgcg
     4801 tcctcctttt cccagggcga ggccttcatc tcctcgaacc ccgagcgcac gatcaccttc
     4861 acgtcctcgc cgccgatgcg cttggaggtg cggcagcagt ccatcgccgt gttgccgccg
     4921 cccagcacga tgacgcgctt gccgattccc gtcgtgtgcc cgaacgacac cgaggacagc
     4981 cagtcgatgc cgatgtggat gttcttcgcc gcctctttgc gccccgggat gtcgaggtcg
     5041 cgcccgcgcg gcgcgcccga gccgacgaag atcgcatcga agccctcggc catgagcttc
     5101 gtcatcgact cgatcctgtg gccgcccttg aattccacgc ccaggtcgag gacgtagccc
     5161 acctcctcgt cgatcacttc gagcggcagg cgaaagcgcg ggatttgcga ccagatcatg
     5221 ccgcccgcgc gcgggtcctg gtcgaaaacg gtgacttggt agccgagcgg cgccaggtcg
     5281 cgcgccacgg taagggaagc cggcccggca cccacgcagg cgatgcgctt gccgttcttt
     5341 tgcttcgccg gcctgggcat gcgcgccttc acgcctgcgc tgtccttgtt gtcggcggca
     5401 acgcgcttca gccggcagat ggcgacgggc tcgggcctgg cctggttctc ttcctcgacg
     5461 cggccgcggc ggcaagcggg ctcgcaggga cggtcgcagg tgcgcccgag gatgccgggg
     5521 aagacattcg acttccagtt gaccatgaag gcgtcgtcat ggcgccctgc agcaatcaag
     5581 cggatgtatt cgggtacggg ggtgtgggcg ggacacgccc actggcaatc gacgactttg
     5641 tggaagtagt cggggttcct gatgtcggtg ggctgcaact cctcgtctcc ttggcggcgc
     5701 cgcttccgtt gcgggcgcgc gacggctgcc gcacaaggac aagtctactc cgggataacg
     5761 cggggggaaa ggctttgtat tcaaacccct gcgacgaggc gggttacccc tcggcgagcg
     5821 tgaaaaagaa ggccgcgccg tgctcgggct cgccttcggc ccaggcccgc ccgccgtggc
     5881 gctcgacgat acgcttcacc gtggccaggc cgatgccggt gcccggaaag tgctcctcgc
     5941 tgtgcaagcg ctggaaggcc ccgaagagct tgcccgcgta ggccatgtcg aagccggcgc
     6001 cgttgtcgcg cacgaagaag gcgtgctcgc ctgcggcgcg ctgctcgccg acctcgatgc
     6061 gcgccccctc gcgccggcgc gtgaacttcc aggcgttctc gaggaggttc tgcagcacca
     6121 cgcgcagcag cgcggggtcg ccgcgcgcgg ccagcccggg cgcgatcgcg atctcgacgg
     6181 cgcgccgcgg ctcgccggcg cgcagctcgt cggccacctg cagcgcgagg cgcgacaggt
     6241 cgaccggctc cggacgcacc ggcgcccgcg tcacgcgcga gagctcgagc aggtcgtcga
     6301 tcagccgccc catgcgcgcc gccgcgtcct gcacgcgctc caggtagtgc cccgcctggg
     6361 cgtcgagcgc cgcgccggcc tgctgcgcga gcaggtcgct gaagccgtgg atcgcgcgca
     6421 gcggcgcgcg caggtcgtgc gataccgagt agctgaagga ctcgagctcg tcgttcaggc
     6481 gctgcagctg cgcggtgcgc tggcgcaccc gcgcctcgag ctcggcgttg agcgtgcgaa
     6541 tctccgcctc ggcgcgctgg cgctcggtga tgtcgcgcac cacggcgatg ctgcccgcct
     6601 cgccctggta gacgtgcggc acgacggaaa cctcgccggt gaacagcgtg ccgtcgcggc
     6661 gcaggaaatt gcggaccgtg ggcgggtagg acgccccttc gtcgcgcacg cggcgcatgc
     6721 gctcggcgac gtcggcgcgc tcgtcgccgt ggatgtactc gagcagcggc cgcccgacga
     6781 cgtccgcggc gcgcgactcg ccgaacaggc gcaacgcggc cggattcacg tagcgcagga
     6841 tgccgccgac ctgtacatag atcgccgcgg gcgagacatc ggtgagcgcg cggtagcgcg
     6901 cctcgctctc gcgcagcgcg cgctcggcga tgacgcgcgc ggtgacgtcg cggctgatgc
     6961 cgcggtagcc gatgaagcgt ccgcccgcgt cgagcattgg ctgcccgctg atcgccacga
     7021 agcattcgct gccgtcgccg cggtagcggc cgacgatgaa gtcgcggaac ggctcgcgcc
     7081 gctccagcag cgcacgatgc tgatcccact gtgacgcgct cacgccatgg tgcggcgtct
     7141 cccagcgcgg cctgcccaga tagtcgctcg ccgccatccc cgcggcctcg agctcggccg
     7201 aggtgaacac cgtgaatttc atgtccgcgt cctgctccca ataccagtcc gccgacaggg
     7261 ccagcagttc gttccagtcg atgccgctat tcaacgcgcc tcgcctcccg ctcgaggcga
     7321 taagcgtagc ggaaatcagg ccgcctcggc ctgctcccgg gggcctgcca gtcatgtgag
     7381 cgggcggaat atccgcacgg caccgggtcc gggtaagcta tgcgcatgcc cacccagctc
     7441 gaatccctgc gccggcactc caccgtcgtc gccgacaccg gcgacatcga cgccatcgcg
     7501 cgctggcgcc cgcaggacgc gaccaccaat ccgtcgctgc tgctggccgc ggcgcaggag
     7561 gcgcgcttcc gccccctggt cgacgaggcg ctcgagcgcg gccgcggcgc gcccgagccc
     7621 acgctggagt ggctgttcgt gctgttcggc cgcgagatcc tgaagcacat gccggggcgc
     7681 gtctccaccg aagtggacgc gcgcctgtcg ttcgacgccg cgggctcgat cgccaaggcg
     7741 cgccgcttca tcgagctcta cgaccaggcg ggcgtggcgc gcgagcgcgt gctcatcaag
     7801 ctcgcgagca cctgggaggg gatccgcgcc gccgagaagc tcgagcgcga aggcatccgc
     7861 tgcaacatga cgctgctgtt ttcgttcgcg caggccgcgg cctgcgccga cgccggcgtc
     7921 gcgctgatct cgccgttcgt cggccgcatc tacgactggc acaaggcggc gcgcaaggtc
     7981 gacgacatcc cgatcgcgga agaccccggc gtcgcctcgg tgacgcgcat ctaccagtac
     8041 tacaagaaat tcggctacgc cacgcaggtc atgggggcga gcttccgcaa gaccgggcag
     8101 atcctggcgc tcgccggctg cgacctgctc accatcgccc cggacctgct ggaaaagctc
     8161 gccgagaccg agggcgaggt ggcacggtgc ctgtcgccgc aggacgccgc cgcgcagggc
     8221 ttcgagcgcg tcgcgctcga cgagaagggc taccgctggc gccacaacga ggacgccatg
     8281 gcggtggaaa agctggccga gggcatccgc aggttcgatg ccgacgcgcg caagctcgag
     8341 cagttcgtgc tgccgctcgt caaggagcgg cgcgccgccg cctgacgcgc ttggccgcgc
     8401 cgatccagac cacgctcgag gagcgcgccg gcgggagcgt ggcgcgggtc acactcgcca
     8461 acccggcgaa gctgaacgcg ctcgacgcgc aggcgatcct ggcgctcgcc gaaacgatgc
     8521 gcggcctcgc cggcgacctg aagctgcgcg ccgtggtcct caccggcgcg ggcgacaagg
     8581 cgttctcggg cggcgtcgac gtggccgagc tcggcgcgac gacgccggcg accgcgcgcg
     8641 aagccatcac ccgcctgcac cgctgcatca ccgcggtgcg cgagtgcccg gtgccggtga
     8701 tcgcgcgcgt gaacggctac tgcatcggcg gcgcgctgga gctggcggca ggctgcgacc
     8761 tgcgcatcgc cgccgaccac gcgcgcttcg gcatgccgga ggtgcgcctc ggcatcccgt
     8821 cggtgatcga agcggcgctg ctcgcgcgcc tgatgggcgc cgggcgcgcg cgctggctgc
     8881 tgctcacggg cgagctgatc gacgccgccg aggcgctgcg ctgggggctg gtggagcgcg
     8941 tggtgccggc acagggcctg gacgccgagg tcgaccatgc gctcgagtgc atcctggaga
     9001 gcggcgccga ggcgatccgc gcgcaaaagc ggctgaaccg cgcctacgag gagctgccgt
     9061 ttgccgaggc ggtggcgcag agcatcgacg ccttcgagca gtcctaccgg acgggcgagc
     9121 ccgcgcgcca gatcggggat tacttgcggc gcaggcggaa gtagagccaa agcaccgcgt
     9181 agagcaacgc cggccccgcc accgcgagca ccatcggctg cgcggctttc gccccctcgc
     9241 cctcgctgaa ggcgaggatg gtgacggcgt tcagcacgca cacgacgacc cagatcgccg
     9301 agccgagcag ccagaagcgc ttccagagcg gcatcagccg atcggcaggc gccgcagctg
     9361 ctcgaggtcc tgcacggcct tcgcctcggc ctccgtcgcc gcctggtgca ggcgccggcg
     9421 ctccgcttcg gcgagcagcg gcgcctcggg caccacggcg cgcagcttct gctgcgactc
     9481 cccgtgcgcg tagtagagcc ccgcgagcgt ccccaccgcg gtgcggtagt cgaagttgtc
     9541 gcgctcggcc ttttgcttca gcgccagcgc gcgctggatc caggccgcgc cgcggccctc
     9601 ggcgtgctcc atgtgcgcgg cgagcgcgcg gcggctgcct tgcgccgcgg caagcgcgcg
     9661 cgcggcgttc gcctgcacgc gcagcatcgc ctgcacgtcc accaggtaga gctcggcggc
     9721 ttcggcgagc gacttgttgc gcgaggcgtc ctcggcgcgc aggcgctcga ggtgcgcgcc
     9781 gagggccgct tcgtgcgcct gaagctgcgc ggcggccttc ggcgcgtcga gcggcagcgc
     9841 ggccgcgtcg cgcagccgcg ccgtggcgtc ggccacgctc gccgcggcgc tcgactcgag
     9901 ggcctgcttt ttcagcgagc cgtagtagaa gctgccgccg atgacgagca cgatgaggcc
     9961 ggcgaaggcg ccaagcagga gctggctgcg ggaggacgag cgcatggccc ccggatttta
    10021 cgccgcctga taaagtcgac gctgagccga aaactgcccg aggcccgtga tgcttcccga
    10081 actgaaatcc gccgccgaca tcacctaccg cgaatccggc aggaaggagg cacccgcgct
    10141 cgtgctgctg cacggcatcg gctcgacctc ggcgggctgg cgcctgcagt acggtcccct
    10201 gggcgcgcat ttccgcgtca tcgcctggga cgcgcccggc tacggcggct cgaagccgct
    10261 cgcggccgac gcgccttcgg ccgaggacta cgcgcgcgcg ctcgcgcgcc tgctcgatgc
    10321 gctcggtgtc gagtccgcga tcatcggcac caactcctgg ggcacgccca ccggcgtcgt
    10381 gtttgcacga ctgttcccgg cgcgcgtgcg cgcgctggtg ctcggcgggc cggcggcagg
    10441 ctggggcgcg gcaccgcagg cggagcgcga gcagcgcatc gccgcgcgca tcgagcgcgt
    10501 gtccacgctc ggcgtgcaaa ggatgcgcgc ggacgacgcc gcggagctcg tcgcccccgg
    10561 cacgcgcgcc gaggtgctcg catggatcaa gggcgccgaa gggctgaacg cgagcggcta
    10621 tgcgcaggcc gcgcgcatgc tcggcgcggt cgacgtgccg cgcgaggtca ccgcggtcgc
    10681 ctgcccggtg cgggtcgtgt cggggacgct cgaccggcgc acgccgcccg agaccaacgc
    10741 caagcgcatc gccgccgcgg cccccaacgc cacgctgcag atggtcccgg actgcgggca
    10801 cctgccgcat ctcgaacatc ccgaggtgtt caacgccgcg gtgctggcga tggcgcgctc
    10861 gctcggcgag gcgcgcgcgg cgtgaatgca ccgcaggccc acgagttcct cacgctgcac
    10921 gagatcgtgg cggcggcgcg cgccgcgctg ccgcccgggc cctgggccta cctgatcggc
    10981 ggcaccgaaa ccgagaccac ggtgcgccgc aaccgccagg cgctcgattc gctcgcgttc
    11041 cggccgcgcg tgctgcgcga cgtgcggcgc gtcgccgcca ccgcgagctg cctcgggcgc
    11101 aaggtgcgcc tgccggtgct gctggcgccg atcggcggcc tggagtcgct ggtcgagggc
    11161 ggcgcggcag ccgcggcgcg cggcgccgcg cagttcggcg tgccgcagat gctgtcctcg
    11221 gtgtgcaagc cggggctgga ggagacggcg gccgccgcgg acgcgctgcg catcttccag
    11281 ctctatgtac gcggcgacga cgcctgggtg gacgattacg tgcgccgcgc ccgcgaccac
    11341 ggctacgcgg ccttctgcct cacggtggac gtcgccatgt acagccggcg cgagcgcgac
    11401 ctcgtcgggc gcttcgtcaa gccctggcgc gcggacgccg cgcagggccg tgatttccag
    11461 gccgcgctcg cctgggacca ggtgaagcgc tacaaggacc ggcacgacct gccgctcgtc
    11521 ctcaagggca tcgccaccgc ggaggacgcg cagatcgcgg tcgagcacgg cgtcgacgtg
    11581 atctacgtct ccaaccatgg cgggcgccag ctcgaccacg ggctgggcag catcgcggtg
    11641 ctgcccgagg tggtggccgc cgtgggcggc cgcgcccagg tgtgggtgga cgggggcttc
    11701 atgcgcggca cggatgtggt caaggcgctc gcgctcggcg cgcaggccgt gggcctgggg
    11761 cgcctcgcct gcctcgggct cgccgccgcg ggcacgccgg ggctcgtgcg cgcgctcgag
    11821 atcctcgagg aggagatcgt gacctgcctc gggctcctcg gcgccacggc gctcgccgag
    11881 ctcggcccgc gccacctggc gagcgcggcg ccggtcggcc cggccggcgc gctgagcgcg
    11941 tttcccctgc taggagaata attctccgag cgggtatgct gcgcagcgga ggaaagtcgg
    12001 caccgtggca ttgaagacac ttgggcgcta ccgcatcctc ggcgagctgg ggcgcggcgc
    12061 catgggcgtc gtgtaccgcg cggtcgatcc ggtgatcgag cgcgaggtgg cgctgaaggc
    12121 gctgcacgcc gagctgccgg ccgacgtggc cggcgaggtg cgcgtgcgct tcctgcgcga
    12181 agcgcgctcg gccgggcgcc tcaaccatcc caacatcgtc accatcttcg acgtcggcga
    12241 ggacgccggc gccgcctaca tcgcgatgga actgctcgac ggcccgtcgc tcgcgcagat
    12301 gctgaaggaa cgccagacga tcgcattcca cgtcgccgcc gacatcatcg cccaggtcgc
    12361 ggatgcgctg caccacgcgc accagcattc gatcgtgcac cgcgacgtga agccggccaa
    12421 cgtcgtggtg tcgcgcacgg ggcgcgccaa gctcaccgac tttggcgtgg cctatgtccc
    12481 ggcgtccgac gtcacgcagc acggatcggc gctcggctcg ccgcgctaca tggcgcccga
    12541 gcaggtgctc ggccagccga tcgacgcgcg cgccgacgtg ttctcgctcg gcgtggtgct
    12601 gtacgagctg ctcacgcggc gcacgccgtt cgagtggccg ggcgacacca cggtgtttgc
    12661 gctcatgcag cgcatcgccg gcgagccgca tccgccgctg cggcagatcg acgccgcgat
    12721 cccggccggg ttcgaccgca tcatggaccg cgcgctcgcc aaacggccgc aggaccgcta
    12781 ccagagcgcc gccgagctcg cggccgacct gcgcaactac cacagcctgg gcggcggcac
    12841 cgagttcgcc aagaccgtgc cgcaggagac cctgcggccg gcgacggtgg tggccgaaga
    12901 gcagagcctg cgcaaccagc tgctcgacga cctcgagcag ttcgcgaagg acttcgagac
    12961 gcaggaggtc gcgcggctgc gcgcggagca ggaagcgcgc ctgcgcaagg agcaggcgct
    13021 gcagcgctgg ggcgcggccc aggcgcacga gcgcgaggca ttcgagcgcg gcgagcgcgt
    13081 ggtgcccgac gaagacccga agagcatgac caccacgcgc cgcatggagg ccgtggagat
    13141 catgcggcag caggcggcct tccgcaaggc gcaggccgag gcgaagccca aggcgatggc
    13201 ggcgctcgac gccgcgatgc gcgcggtgct gcagtacctg accgagttcg gcaaggagat
    13261 gcaggcgcac gcgccgcacg cgggcgcgcc ctacgagttc atctacgtcg gcaaggtgcc
    13321 ttcggtgacg ctggtccagc ccacggtcag caaccgcacg ctgaagctgc ccgagaacaa
    13381 ggagctctgc gcgcagatcc agttccgctt ctgggtgcag ccggcggagc cgccgaaatt
    13441 catgctgctc ggcgaggatg tcacgcgctg cgagcagtac ctgaagtcgc tgcgcgtgag
    13501 cttcgagaag cgcgcgcgcg gcgacgacaa ggagcgcccg gtgcagttcg tcgtcaacgg
    13561 acccctgccc tgcgacgtca acgtgcgcgc ggactacgag gcattgaccg ccaccctcga
    13621 gctgatcaac gtgcgccgct tcggccgctt cacggtgcgc ctggacacgg cgtcgttcaa
    13681 ggcggcgatc gacgatctgg cgcgctacgt gctcggcgtc gacgacgagt tcgagaagct
    13741 gatccccgag gcctagtccc tccactcacg gtacgggcgc gagggtctgg ggggctggcg
    13801 gtggcgctgg cgcatggcga acccccacag gccggcgatg ccgacgatga cgccgaggcc
    13861 gaggccgcgc agaaggccgc atccttcgat accgcaggcg acaaggcgca ggcgaatttc
    13921 cggcgccgcg tgggcgaggc gctgcaggag atgcgcgggc cgcgcgagag ctagtcgccg
    13981 gggtcgtgaa gcagtgcgct gcgcgcgcct aggccgcgcg gaattcggcg atgaggccga
    14041 gcatttccgt ggcctgatgc ggcatgcgcg cgcccttcat cctgaacgcg gagcactgat
    14101 ccttcatcat caggcgaaac gcgctcatca ggtagtcccg cttgaaaccc ttctcgagcg
    14161 cgagcacttc cttcagcttg tgggcgtaga actccatgta gcaattcgcc aacgcctcgt
    14221 tgacctcctc gagagacatc tcgaagggct cgatgatcgg ggtcaccagg ttgtacttgg
    14281 catagtcaaa caccctgatc ctgtccttca tctccttgta catcggcgta tagggccacg
    14341 gcgtcacgat cgggaatacg gcgacgtcgg gattcaggta gatcgcggcg tcgatggtgt
    14401 tccggacgga gctcggcgtt tcctgcggaa agccgatcat gaacgaggct tcgctgatga
    14461 tgccggcctg gttgagcagc gccagcgcct gcttggtggt cttcatctcg atgcccttgt
    14521 tcaggctcgt gaggaccgcg tcgtcgccgc tttcggcccc catgtagatg tggatgacgc
    14581 cggctttcct gtatttgtgg aggatgtccg catcgcgcac gatgtcctcg acccgcgttt
    14641 ccatgagcac ggacaatccc aaatcccgtt cgatcaacag gtcgagcagc cgttcccagc
    14701 gctcgcggtc gcgcgtggga aaggcatcga tcagggtgaa taccgtgacg ccgtaccttt
    14761 ggtgcagcag aacgatttcc tcgacgacct tttcgggccg ccgggcgcgc caggcgccgc
    14821 gccagaacag cctctggctg cagaagctgc agtgcatctc gcagccgcgc gaactcaata
    14881 tggaggccat gcgtccgccc ggactgaccc ggtagcggta agcgtcccag cgcaggagat
    14941 cccaggcggg ctgcagttga tccagatcgg cgatcagcgc ccggctgcca gtgccgaccg
    15001 cgcgcgcgcc gtcgaggtag gcgatgccct tgacgccgct cgccgatccg ccacgcaagg
    15061 cctgcagcag ggccttgagc gtcgcctcgc cctcccccag gagaacgtag tccacgacgc
    15121 cccgctcgga ctcgagcacc tccttggaca tgaacgtggg gtgggggccg ccgagcagcg
    15181 tcacgatgcc cggatcgatc tccttggcga gcttcaggat cgcgagcgac gccggcagcg
    15241 cggcggtact gatcgcgccg gtcaccggaa gataatcgta ggccagcacg taatccggcc
    15301 tgaaactctc gatctgcgcc cgaatgtcgg cgtacgagtg cgactcgttc atcgcgtcat
    15361 ggatcttcgg caccacgccc gcctcgcgcg cggcgcccgc cagatagacc aggtgcaacg
    15421 gcggccacgt gccgaccacc tggacgcccc agcattggta aggcgcagtg accagcagta
    15481 tcttcttgtc catcagttcg tcgcctgcgc cttgaggcgc ttgaggtgct tgcgcagacg
    15541 gtgctggata tacgtgcccc agacgacgac cgcgacaagc aggacgtgct tcaccacgag
    15601 cgccgccacc tggccgcggc ccgccgcctc gttccactcg aagctctcgt aggccagcgt
    15661 gcgcccgatg ccgccgagga tcagagccgc ccagcatgcg taaaccacct tctcgaaggt
    15721 gccggccacc acgcgcacca gcgcggtatc ggccggatcg ccctgccccc ggatgcagcg
    15781 gtagaacaga ccgagcatca gcaggctggc gaacagcagc gccacggcga agtcgtggaa
    15841 caggttgttc agcatgacga ggatcgcgag ggtcgaatcg ctcatggtcc gtgtccgccc
    15901 gatcagtact tgtacagcgc gatggcgcgc agctgcacgt cggagatgta aatgagccgc
    15961 tgcttcgggt cgatggcgat gccgttcggc tggcgcagct cctcgccgta ggtcagcacg
    16021 tacttgccga gcgcgtcgaa gaccttgacg cgcgcctgcg ccgcgtctac gacgtggatc
    16081 cgacccagct catgaacgcg cgaggggtcg aacacgcgtc ttgctttggc cagcagctcg
    16141 ggcgagcgca ggtcgtccat ggcgatgccc atcggcgtct tgagacgcgc cggctcggcc
    16201 tggtcgaaca gatgcgccga cctgccgctg caggtgaacg ccttcacctc gccgctcttg
    16261 gcgtcgctca ccagcaggcg gccatcggcg gtcaccattg accaggtcgg gtagcccagc
    16321 tcgaattccg atggcccgcc gttgggaaaa gcgacgatca gctcgccttc gtcccgcacg
    16381 tccttttgcg ccagcgcgcc gatcgcgagc atcttgccgg aggcgacgtc ggagacatac
    16441 agattgttct ggaaataggc gacgccgagc gccttcatgc gcgtctggta accgggcagc
    16501 cagccgtaag attgcagcag cttgccggtc ttgtattccg cgaccagaag atcgcctgaa
    16561 acgaaatcgg tcgcgtagat gcggtcgccc tccaccgcga cgccggtgat cgacgcgggc
    16621 tcgttctgcc gcaggtgcag cgtgcgcctg aattcgagcc tgtcggaaaa ctcgtcgacg
    16681 cggtccgtgc tcagatacga caccagcagc ttcttctcgt tcagcgccag cgcgaagggc
    16741 ttgagcgcgg cggcctccgc aacgccgtgc atcccgacga atattggcgg ctggtcgaac
    16801 gccgtgggct cggtaatggc gtcggcgcgc tccatcggcg agcgggtaaa gtacgcgacg
    16861 gaccatccga ccagcgccgc gacgagcagc acggccagga tcgtccattg cggtcgggtc
    16921 aactgccagc gcacggctca attgcctcca taggtatgca gggttctgta cagaaagccg
    16981 ataccccata gcgacatcgc gacgaacagc gcgccgatga tcgcatacca cgcaagaaac
    17041 acgccccgca gcctcctcca taccgtgtag atgtggaggt agaccgcgta caccagccag
    17101 ctgatgaggc tccagacctc gaccggatcc caggcccagt agcggcccca ggccccgtcc
    17161 gcccagatcg cgcccgacac ggtcatgatg ccccagaaca ggaagcccag caggatgagg
    17221 cggaagttga attcgtcgta gcgatcgccc ggcgcgatgt gataggcggg atccgacggc
    17281 gcgttgcggc gctgctggta cagtcgcatg cccgccacgc ccgcggccaa cagaaagcag
    17341 cctgccgccg ccgttgcaaa tcccgcgtgc acgaaaagcc agaaactctg cagactcacg
    17401 ggcagcgact ggccgccgaa cggacgcgta ccggcccagc ccatgagcag cacgatcagg
    17461 ggcatcaggc ccgcgccggc cgggcgcaga aacggcttcc actgctgcag cgccaggaac
    17521 gcgagcatgc cgaaccaggc gctggccgac atcgattcga agtaggaaac gaaggccgga
    17581 tggcccgtct gcacccaccg cgcaaccatg acgacgctat gcgcgaagaa cccggcccac
    17641 gctacggtga tggcgtggcc gagcgtgccg ggcttgcgaa acaccagcgc gacggcgaac
    17701 aggccgaagg cgatcgcata gaccacggcg gcgagccaga acaacgcgga aagcacggtc
    17761 tgcgaggtca tagggctttc aagtccaggt caaagacatg gaacgtcctc cccgccggga
    17821 ggtagatcag cccccagtag gagaagccga tcacggcgaa gatcagcagg tacgaccgcg
    17881 ccgcccgctt gctgttccag tggtagaaga gccgcaggtg gatatacagg ccgaccagca
    17941 gccaggccac cagggaaagc gaaaaggccg ggtcccagcg ccaccaggtg ccgaacagga
    18001 tggaggaata gtagctgccg agcgcgaaca tgacgctgtg ggcgacgaaa cccagcgtga
    18061 tgtaacgaaa cgcgagctcg tcgaggatgt ccgcgtgcgc cgggggcggc gcagccgcgc
    18121 ggcggcttcg ggtcgaccac agataggacc ccgacaggca gaacgccagg gtgaacggcg
    18181 caaaggcgag ccaggaaaag agcgcgtgga actccaccca caggctgcgt tccgaaatgg
    18241 tcagcgggat cctgctcgta tcgaagaagg tcacgccgta cgccagcagg agcagcgcga
    18301 tcgggacggc ggtcaggacc agcagcctga attgacgatg caccgagccg aaggcaagcg
    18361 cgacgaagag catcagaaac cagctcgcgg caagcgtggc ttcgaaggtg ccgaagaccg
    18421 gcgggtgccc ggttccgacc catcgaatgg ccgcggatgc aagatgaagt gccaagccgg
    18481 ccacgacgct gacaaagcca taacgcaacc atccttttcc agccagaaga aaagacgagg
    18541 cgtacagcgc gccggcgccg aaaagcaggc cggcctcgag ggaagtcatg cggcttccgc
    18601 cttgaattgc tcgcccaggc tcgcgagcca gccggcgttc agcctcggga aataatcgaa
    18661 gcgcccggac agccaggtgc ggccggcgtc atcctgccac agcgcgatgt tcttccggta
    18721 aaagagcagt cgccatgcca ggcccaggcc catgacgatg aaggcggccc agatgatcgg
    18781 atgccccgga ttgctgacga tttgaaaggt ccccgacttg acgtacgatg gaaaggaaat
    18841 gctcaagccg tcgaagtccg cccactcccc cggcttgatg gcgccagtga acacggggag
    18901 cctgccgcgg aatatcctca acatgtacac ggggttgacc ggatgcatcg acttgctgac
    18961 ggcttttccg tccacttgcg catggtcggg ataaaaggaa acgaagatct tgtggggata
    19021 ttccggcacg tggaaatagt cttcgctgcc aggggggaag atgttcagtt tgacg
//
LOCUS       DBNHKE010000256         8792 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_158514, whole genome shotgun sequence.
ACCESSION   DBNHKE010000256 DBNHKE010000000
VERSION     DBNHKE010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_158514"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="AC16O9_17230"
     CDS             complement(<1..1394)
                     /locus_tag="AC16O9_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6518394.1"
                     /translation="MLLTRKASEQAVPQSRLARGLSGVLAKTMDRRAFLRRSGVTLGA
                     GAAAAHLPFSMIGEAQAQAKGAGKMEVKRTVCTHCSVGCAIDAVVENGVWVRQEPVFD
                     SPLNLGAHCAKGASVREHGITHDSHRLKSPMKLEGGKWVKVSWDQAYNDISQKLLAIR
                     KESGPDALMIIGSSKHNNEQSYLLCKWARLWGTNNTDHQARICHSTTVAGIANTWGYG
                     AMTNSYNDEQNSKCLLFFGSNAAEAHPVSMLHTLHAKENGAKVIVADPRYTRTAAKAD
                     KFVRVRSGSDVAFLMGLMYHIFKNGWEDKKYIHDRVYGMDKVKAEVMAKYTPAAVEEV
                     TGVGEKEMYETAKMLSDNRPGFIIWAMGQTQHTNGNAIVRASAILMLALGNAGRGGGA
                     NIYRGHDNVQGATDIGPNPDSLPGYYGLAEGSWKHWCKVWGVDYEWMKGRFQDKAMME
                     KPGITVSRWIDGVL"
     gene            complement(1405..1602)
                     /locus_tag="AC16O9_17235"
     CDS             complement(1405..1602)
                     /locus_tag="AC16O9_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HIR6518395.1"
                     /translation="MVQKNAKLSRRNFLLTVGAGGAATAAAVVAGGKALQGTSAASGK
                     QGSRGYHETAHVNNYYRTAKV"
     gene            complement(1768..2397)
                     /locus_tag="AC16O9_17240"
     CDS             complement(1768..2397)
                     /locus_tag="AC16O9_17240"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR6518396.1"
                     /translation="MSVPLQFVPTATPEDQARAQLYGLVASLFYSAPDAQLLSALVHA
                     EGFRGEDDERTAQGRALAGAWRELSEACRSAFPVLLAHEHTDLFVSAGRAEVTPYLMH
                     YVMRYESETPLVDLRRQLAEWGIARHESVHEPEDHISALCETMRFAIAVQQRTLEEQK
                     AFFNRYLYRGAIAFCDAVRASDKARFYKSVAGFARAFLEVEREAFDIGG"
     gene            complement(2394..4511)
                     /locus_tag="AC16O9_17245"
     CDS             complement(2394..4511)
                     /locus_tag="AC16O9_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6518397.1"
                     /translation="MALQDKTIKLCSCNGTVALDAKALAAALKQGLPLSVSTELCRKE
                     AGVFQAALGEPEVLVACTQEAALFSELAQAAEAKGRLDFVNIREAAGWSAEGKRATPK
                     IAALLALAALPEPEPVPNVEYKSAGEVLVIGPSAAALDWAGRLAAELGGALEPSVLIT
                     DCKGGELPAERTYPVWSGKPVRASGHLGAFEVEWRQENPIDLELCTRCNACLDACPEN
                     AIDFTYQIDLDKCKAHRKCVAACGALGAVDFSRTDTARRETFDLVLDLSRVPLLAMAD
                     LPQGYAAPGADPLAQALAAAKLAQLVGEFEKPRFFQYREAICAHGRSGKTGCTRCLDV
                     CSTGAISADGDHVKVDPHLCTGCGGCAMVCPSGAMTYAYPRAPDFGRRLKTLVGTYRD
                     AGGTDACVLVHDLEQGRALTYGVGRRAIRGGKGLPARVLPLAVYHPASIGIDALLGAV
                     CYGASQIVVLMDAAQPEAYAAALEEQMRFAQAILNGLGYAGTHFTVLRPADAKALEGA
                     LWALEPAQSVGKAATFNLSPEKRTTLDFAFEHLAKQARAPKEVLELPAGAPFGALTVN
                     KDTCTLCKACIGACPEAALLDAPETPSLRFIERNCVQCGLCEKTCPENAIRLVPRLLL
                     GAAAKDPVVLNEAEPFNCVRCGKPFGTRQMVDNMLGKLGAHSMFSGEGALKRLQMCGD
                     CRVVDMMQNTKEASIFDVAGRKP"
     gene            complement(4495..4950)
                     /locus_tag="AC16O9_17250"
     CDS             complement(4495..4950)
                     /locus_tag="AC16O9_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HIR6518398.1"
                     /translation="MAQDRDDFLHRWSRRKQDAAAQAKAPAKAAAADPAPKLPSLDSL
                     TFESDFKDFMHAKVEQSVKRAALKKLFADPRFNVIDYMDVYIDDYTNLETIPPDMLAR
                     LDHAKGTLFKRDDETKDAARQEPAEPETLAQAPEAPQEEEPKKDDGAAG"
     gene            complement(4950..5420)
                     /locus_tag="AC16O9_17255"
     CDS             complement(4950..5420)
                     /locus_tag="AC16O9_17255"
                     /inference="COORDINATES: protein motif:HMM:NF023177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HIR6518399.1"
                     /translation="MNNPTFPVSIVMQRRAIQHRWQSDVWEPFAVLPGAEKGGPRVLA
                     DTPEATRWLYPGMEIMLRVADADGYFLNVSTSEPCVFVLWRMEGERAVPAHLTVSYSE
                     ASSWMDGGEEVDRVPMTPELFAWVSDFVKAHYRPQPKKKIRPQSFKSPKDRARG"
     gene            5479..6543
                     /gene="moaA"
                     /locus_tag="AC16O9_17260"
     CDS             5479..6543
                     /gene="moaA"
                     /locus_tag="AC16O9_17260"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6518400.1"
                     /translation="MTQKVFPIHPARAPATAYDGRPLADALGRGLRDLRVSVTDRCNF
                     RCVYCMPRATFDRSFKFLPHAEILSFEEIARLARLFAGLGVKKIRITGGEPLVRRELH
                     RLVAMLAEVPGLDLTLTTNGSLLAKQAGALAAAGLKRVTVSLDSLDDATFRAVNDADY
                     PVARVIEGIEAAAAAGMAPIKINMVVQRGVNDGHVLAMAERWRGTGHILRFIEYMDVG
                     STNGWRMDDVVPSAEIVKQIGARYPLEPADPNYAGEVAERWRYRDGAGEIGVISSVTQ
                     AFCASCNRLRLSAEGSLYTCLFAERGHDLKWLLRRGSPDAEIVNEIAAVWRARADRYS
                     EIRTAETAKARKVEMSYIGG"
     gene            6536..7381
                     /locus_tag="AC16O9_17265"
     CDS             6536..7381
                     /locus_tag="AC16O9_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR6518401.1"
                     /translation="MARKPLRPELTDAAKPATFEVEAYNERGEMVPTAVAGEHPLTLY
                     LDKKELVTLMTLGHAPEALAIGYLRNQNIVGSIEEIAAVQVDWETESCAITTRKGVKG
                     LKAKMGRRTVTTGCGQGTVYGDLMEQIAQVRLRADVTLADEQLYVLIDKVRKHETIYK
                     QAGAVHGCALATRAGDIMMFVEDVGRHNAVDAIAGFMWLQAIDGSDKVFYTTGRLTSE
                     MVIKCAQMRIPFLVSRSGLTQMGYAIAQKVGITMLGRASGKHYLAFTGRERLLRQSPQ
                     PTAYA"
     gene            7396..8055
                     /locus_tag="AC16O9_17270"
     CDS             7396..8055
                     /locus_tag="AC16O9_17270"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6518402.1"
                     /translation="MVELYFAPGACSFVPHVGLEAIKAATGADFKPNLVKLHKGEQKT
                     PEYLALNPNGQVPVLVVDGKPLSQIVAVCDYLDRSFPKARLLPTESWSRAQALSQLAW
                     MNNTVHPTFTHWFRPYEFAQSEAAQADVKAAAVERFRKHLERLQEWSKAAAPYWFGDQ
                     VSFHDAYCFTLLRWGGYAGIDPDSVPRLKAYVERVIQAPPVAAALERERVKLDTYKKA
                     A"
     gene            complement(8078..>8792)
                     /locus_tag="AC16O9_17275"
     CDS             complement(8078..>8792)
                     /locus_tag="AC16O9_17275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518403.1"
                     /translation="FVPGLICVAAGAGFLALVKDPGRVKKTSKSIGFHVDGRTMARIF
                     TVLLVATACGGIIFNSTTVSMPKVFDERLRALTQTNFGIGALVAVVYTIAAFAQVAMG
                     ALIDRFELRRLMIGVALAQIPLLALAANLQGWAMLGAAVLMMLAVFGQIPLNDAIVGR
                     YCADEFRARVLAVRYVVSLGVAAVAVPLIAVLHATEGGFANVFSVLALLAAGMLAASL
                     FFPTRDALVASRARAAQPA"
BASE COUNT         1380 a         3068 c         2909 g         1435 t
ORIGIN      
        1 tcgagcacgc cgtcgatcca gcgtgacacg gtgatgccgg gcttctccat catcgccttg
       61 tcctggaagc gacccttcat ccattcgtaa tcaacgcccc agaccttgca ccagtgtttc
      121 catgaaccct cggccaggcc gtaatagccc ggcagcgagt ccggattcgg accgatgtcg
      181 gtggcaccct gcacgttgtc atggccgcgg tagatgttcg cgccgccgcc acgcccggca
      241 ttgccgaggg cgagcatcag gatcgcgctc gcgcgcacga tggcgttgcc gttggtgtgc
      301 tgcgtctggc ccatcgccca gatgatgaag cccgggcggt tgtcggagag catcttggcg
      361 gtttcgtaca tctccttctc gccgacgccc gtcacctctt ccacggcggc aggcgtgtac
      421 ttggccatca cctcggcctt taccttgtcc atgccgtaga cgcgatcgtg aatgtacttc
      481 ttgtcttccc agccgttctt gaagatgtgg tacatcagcc ccatcaggaa cgccacgtcg
      541 ctgcccgagc gtacgcgcac gaacttgtcg gccttggcgg cggttctggt gtagcgcggg
      601 tcggcgacga tcaccttggc gccgttttcc ttggcgtgca gggtgtgcag catcgacacc
      661 gggtgcgcct cggccgcgtt cgagccgaag aacagcaggc acttcgagtt ctgctcgtcg
      721 ttgtacgagt tggtcatcgc gccgtagccc caggtgttcg cgatgccggc gacggtggtc
      781 gagtggcaga tgcgcgcctg gtggtcggtg ttgttcgtgc cccagaggcg cgcccacttg
      841 cacagcaggt aggactgctc gttgttgtgc ttggacgagc cgatgatcat cagcgcgtcg
      901 ggcccgcttt ccttcctgat cgcgagcagc ttctgcgaga tgtcgttgta ggcctggtcc
      961 cagctcacct tcacccactt gccgccctcg agcttcatcg gcgacttcag gcgatgcgag
     1021 tcgtgcgtga tgccgtgctc gcgcaccgaa gcgcccttgg cgcagtgcgc gccgaggttg
     1081 agcggcgagt cgaacaccgg ctcctggcgt acccacacgc cgttctcgac gacggcgtcg
     1141 atggcgcagc ccacggagca gtgcgtgcac accgtgcgct tcacttccat cttgccggcg
     1201 cccttggcct gcgcctgcgc ctcgccgatc atgctgaagg gcagatgcgc ggcagccgcg
     1261 ccagcgccga gcgtgacgcc cgagcggcgc aggaaggcgc ggcgatccat ggtcttggcg
     1321 agcacgccgg agagcccgcg ggcgagtcgc gactgcggta cggcctgctc ggatgctttt
     1381 ctggtcagaa gcatggcgtt ctcctcagac cttcgcggtg cggtagtagt tgttgacgtg
     1441 cgccgtctcg tgatagccgc gcgagccctg cttgccgctc gccgccgacg tcccctgcaa
     1501 ggccttgccc ccggccacca ccgcggccgc ggtcgcggcg ccgccggcgc cgaccgtcaa
     1561 aaggaaattc ctgcgggaga gcttggcgtt tttctggacc atgttgcgcc tccttgttgt
     1621 gcgtacgccg gctccgctca gcgcggagcg atagaaaact tttgcaaatt accaacctct
     1681 ttgtgcctga tcaagagaaa gaaattccgc aatcgcgaat agttctcgat gacaacggca
     1741 atattgagaa tagttctcga atgtccttta tcccccgatg tcaaaggctt cacgctcgac
     1801 ttcgaggaac gcccgcgcga atcccgcgac cgatttgtaa aaacgtgctt tgtcggaggc
     1861 ccttaccgca tcgcaaaacg cgattgcacc gcggtagagg tagcggttga agaacgcttt
     1921 ctgctcttcg agtgttcgct gttgcaccgc aatagcaaat cgcatggttt cgcacagcgc
     1981 cgagatgtga tcctcgggtt cgtggacgct ctcgtgccgc gcgatgcccc actcggcgag
     2041 ctgccggcgc aaatccacca gcggcgtctc gctctcgtag cgcatgacgt agtgcatgag
     2101 gtagggcgtc acctcggcgc gccccgcgga cacgaacaga tcggtgtgct cgtgcgcgag
     2161 cagcaccggg aacgcgctgc ggcacgcctc gctcagctcg cgccacgcgc cggcgagcgc
     2221 gcgtccctgc gcggtgcgct catcgtcctc gccgcgaaag ccctccgcgt gcaccagcgc
     2281 cgagagcagc tgcgcgtcgg gcgccgagta gaacaggctc gccaccagcc cgtagagctg
     2341 cgcgcgggcc tggtcctcgg gcgtcgccgt cggcacgaac tgcagcggga cgctcacggc
     2401 ttgcggcccg cgacgtcgaa gatcgaggcc tccttcgtgt tctgcatcat gtccaccacg
     2461 cggcagtcgc cgcacatctg caggcgcttg agcgcgccct cgccgctgaa catcgagtgc
     2521 gcgccgagct tgccgagcat gttgtccacc atttgcctgg tgccgaaggg cttgccgcag
     2581 cgcacgcagt tgaacggctc ggcctcgttc agcaccaccg gatctttcgc cgccgcgcca
     2641 agcagcagcc gcggcacgag ccggatcgcg ttctccgggc aggtcttctc gcacagcccg
     2701 cactgcacgc agttgcgctc gatgaagcgc aggctcggcg tctcgggcgc atcgagcagc
     2761 gcggcctccg ggcaggcgcc gatgcacgcc ttgcacagcg tgcaggtgtc cttgttcacc
     2821 gtcagcgcgc cgaatggggc gcccgccggc agctcgagca cctccttggg cgcgcgcgcc
     2881 tgctttgcca ggtgctcgaa ggcgaaatcg agcgtggtgc gcttctccgg cgacaggttg
     2941 aacgtcgccg ccttgccgac gctctgcgcc ggctcgagcg cccacagcgc gccctcgagc
     3001 gccttcgcgt cggccgggcg cagcacggtg aagtgcgtgc ctgcgtagcc caggccgttg
     3061 aggatcgcct gcgcgaagcg catctgctcc tcgagcgccg ccgcgtacgc ctcgggctgc
     3121 gccgcatcca tcagcaccac gatctggctc gcgccgtagc acacggcgcc gagcagcgcg
     3181 tcgatgccga tcgacgcggg gtggtacacc gcgagcggca gtacgcgcgc cggcaggccc
     3241 ttgccgccgc ggatcgcgcg ccggccaacg ccgtaagtga gcgcgcggcc ctgctccagg
     3301 tcgtgcacca gcacgcacgc atccgtgccg ccggcgtcgc ggtaggtgcc gacgagcgtc
     3361 ttcagccgcc gtccgaaatc cggggcgcgc ggataagcgt aggtcatggc gccggacggg
     3421 cacaccatcg cgcagccgcc acagcccgtg cacaggtgcg gatcgacctt gacgtggtcg
     3481 ccgtcggcgg aaatggcgcc ggtcgagcac acgtcgaggc agcgcgtgca gccggtcttg
     3541 cccgagcgac cgtgcgcgca aatggcttcg cgatactgga agaagcgcgg cttctcgaac
     3601 tcgcccacca gctgcgccag cttcgccgcc gcgagcgcct gcgcgagcgg atccgcgccc
     3661 ggcgcggcgt agccctgcgg cagatcggcc atcgcgagca gcggcacgcg ggacaggtcg
     3721 agcaccaggt cgaaggtctc gcgccgcgcc gtatcggtgc gcgagaagtc caccgcgccc
     3781 agggcgccgc aagcggcgac gcacttgcgg tgcgccttgc acttgtccag atcgatctgg
     3841 taggtgaagt cgatcgcgtt ctccgggcag gcgtcgaggc aggcgttgca gcgcgtgcac
     3901 agctcgaggt cgatcgggtt ttcctggcgc cattcgacct cgaatgcgcc gaggtgcccg
     3961 cttgcgcgca cgggctttcc cgaccacacc ggataggtgc gctccgccgg cagctcgccg
     4021 cccttgcagt ccgtgatcag cacgctcggc tcgagcgcgc cgccgagctc ggccgccagg
     4081 cgcccagccc agtcgagcgc cgcagccgac gggccgatga ccagcacctc gccggccgac
     4141 ttgtactcga cgttcggcac cggctcgggc tcgggcagcg ccgccagcgc cagcagcgcc
     4201 gcgatcttcg gcgtggcgcg cttgccttcg gccgaccagc cggcggcttc gcggatgttg
     4261 acgaaatcga gcctgccctt ggcctcggcg gcctgcgcca actcggaaaa cagcgccgcc
     4321 tcctgcgtgc aggcgaccag cacctcgggc tcgccgagcg cggcctggaa cacacccgcc
     4381 tccttgcggc aaagctccgt gcttacgctg agcggcaggc cctgcttcag cgccgcggcg
     4441 agcgccttgg cgtccagcgc caccgtgccg ttgcagctgc acagcttgat cgtcttatcc
     4501 tgcagcgcca tcgtctttct tcggctcctc ttcctgtggc gcctcgggcg cctgcgccag
     4561 cgtttcgggc tcggccggct cttgccgcgc cgcgtccttc gtttcgtcgt cgcgcttgaa
     4621 cagcgtgccc ttggcatgat cgagccgcgc cagcatgtcc ggcgggatgg tctcgaggtt
     4681 ggtgtagtcg tcgatgtaca cgtccatgta gtcgatgacg ttgaagcgcg ggtcggcgaa
     4741 cagcttcttc agcgctgcgc gcttcacgct ctgctccacc ttggcgtgca tgaaatcctt
     4801 gaagtccgat tcgaaggtca ggctgtcgag cgagggcagc ttgggcgcag gatcggcggc
     4861 cgccgccttg gccggcgcct tggcctgcgc ggccgcgtcc tgcttgcggc gcgaccagcg
     4921 gtgcaggaag tcgtcgcgat cctgggccat cagccgcgcg cccggtcctt cgggctcttg
     4981 aacgattgcg gccggatctt cttcttcggc tgcgggcggt agtgcgcctt cacgaaatcg
     5041 ctgacccagg cgaacagctc gggcgtcatc ggcacgcggt cgacctcctc gccgccgtcc
     5101 atccagctgc tcgcctcgct gtagctgacc gtgagatggg ccggcaccgc acgctcgcct
     5161 tccatgcgcc agagcacgaa cacgcagggc tcgctcgtgg acacgttgag gaaatagccg
     5221 tcggcatcgg ccacgcgcag catgatctcc atgcccgggt acagccagcg cgtcgcctcc
     5281 ggcgtgtcgg cgagcacgcg cggccccccc ttctccgccc cgggcaggac ggcgaaaggt
     5341 tcccagacat cgctttgcca gcggtgctga atcgcgcgcc gctgcatcac gatcgacacc
     5401 ggaaacgtcg gattattcat gcgaaggaac tatagctcag aacgttcatt tgctatgatc
     5461 ttccgttcga gaaacaccat gacgcaaaag gtttttccca tccatccggc ccgcgcaccg
     5521 gcgaccgcct acgacggccg gccgcttgcg gacgcgctcg ggcgcggcct gcgcgacctg
     5581 cgcgtctcgg tgaccgaccg ctgcaacttc cgctgcgtgt actgcatgcc gcgggcaacg
     5641 ttcgaccgga gcttcaagtt cctgccgcac gcggaaatcc tttcctttga ggagattgcg
     5701 cggctggcgc gcctgttcgc cgggctgggc gtgaagaaga tccgcatcac cggcggcgag
     5761 ccgctggtgc ggcgcgagct gcaccggctg gtggcgatgc tcgcggaggt ccccgggctc
     5821 gacctgacgc tcaccaccaa cggctcgctg ctggcgaagc aggcaggcgc gctcgccgcc
     5881 gccggcctga agcgcgtcac cgtgagcctg gactcgctcg acgatgccac cttccgcgcg
     5941 gtgaacgacg ccgactaccc ggtcgcccgg gtgatcgagg gcatcgaggc cgccgccgcc
     6001 gccggcatgg cgccgatcaa gataaacatg gtggtgcagc gcggcgtgaa cgacggccac
     6061 gtgctggcaa tggccgagcg ctggcgtggc acgggccaca tcctgcgctt catcgagtac
     6121 atggacgtcg gcagcaccaa cggctggcgc atggacgacg tcgtgccctc cgccgagatc
     6181 gtcaagcaga tcggcgcgcg ctacccgctc gagcccgcgg atccgaacta cgccggcgag
     6241 gtcgccgagc gctggcgcta tcgcgacggc gcgggcgaga tcggcgtcat ctcttcggtg
     6301 acgcaggcgt tctgcgcgag ctgcaaccgg ctgcgcctgt cggccgaggg ctccctctac
     6361 acctgcctgt tcgccgagcg gggccacgac ctgaagtggc tgctgcgccg gggcagcccg
     6421 gatgcggaga tcgtcaacga gatcgccgcg gtgtggcgcg cgcgcgccga ccgctactcc
     6481 gagatccgca ccgccgagac cgcgaaggcg cgcaaagtcg agatgtccta catcggtggc
     6541 taggaaaccc ctgcggcccg agcttaccga cgcggcgaag cccgccacgt tcgaggtcga
     6601 ggcctacaac gagcgcggcg agatggtgcc gacggcggtc gccggcgagc acccgctcac
     6661 cctgtacctg gacaagaagg agctggtgac gctgatgacg ctcgggcatg cgcccgaggc
     6721 gctcgccatc ggctacctgc ggaaccagaa catcgtcggc tcgatcgagg agatcgcggc
     6781 ggtgcaggtc gactgggaga ccgagtcctg cgccatcacc acgcgaaagg gcgtcaaggg
     6841 cctgaaagcg aagatgggcc ggcgcacggt caccaccggc tgcggccagg ggaccgttta
     6901 cggcgacctg atggagcaga tcgcgcaggt ccggctgcgc gcggacgtga cgctcgccga
     6961 cgagcagctc tacgtgctga tcgacaaggt gcgcaagcac gagaccatct acaagcaggc
     7021 gggcgcggtg cacggctgcg cgctcgccac gcgcgcgggc gacatcatga tgttcgtcga
     7081 ggacgtcggc cgccacaacg cggtcgacgc catcgccggc ttcatgtggc tgcaggcgat
     7141 cgacggctcg gacaaggtgt tctacacgac cggccggctc acgagcgaga tggtgatcaa
     7201 gtgcgcgcag atgcgcattc cgttcctggt ctcgcgctcg ggcctgacgc agatgggcta
     7261 cgccatcgcg cagaaggtcg gcatcaccat gctcggccgc gcgagcggca agcactacct
     7321 ggccttcacc ggccgcgagc ggctgctgcg ccagtcgccg cagcccacgg cctacgccta
     7381 ggaggaggat gcaccatggt tgaactgtac tttgcccccg gcgcctgctc gttcgtgccg
     7441 cacgtcgggc tggaagcgat caaggccgcg accggcgcgg acttcaagcc caacctcgtc
     7501 aagctgcaca agggcgagca gaagacgccc gagtacctgg cgctgaaccc gaacggccag
     7561 gtgccggtgc tggtcgtgga cggcaagccg ctcagccaga tcgtcgccgt ctgcgactac
     7621 ctcgaccgca gcttcccgaa ggcgcggctg ctgcccaccg aatcgtggtc gcgcgcccag
     7681 gcgctctcgc agctcgcctg gatgaacaac accgtgcacc cgacgtttac gcactggttt
     7741 cgcccctacg agttcgcgca gagcgaggcc gcgcaggcag acgtgaaagc cgcggcggtg
     7801 gagcgcttcc gcaagcacct cgagcggctg caggaatgga gcaaggctgc cgcgccctac
     7861 tggttcggcg accaggtgtc gttccacgat gcctactgct ttacgctcct gcgctggggc
     7921 ggctacgcgg gcatcgatcc ggattcggtg ccgcggctga aagcctacgt cgagcgcgtg
     7981 atccaggcgc caccggtagc cgccgcgctc gagcgcgagc gcgtcaagct cgacacctac
     8041 aagaaggccg cctaggcgcc cgcggcctga aggccgctca ggccggctgc gcggcgcgcg
     8101 cgcggctcgc caccagcgcg tcgcgcgtcg ggaagaacag gctcgccgcg agcatccccg
     8161 cggcgagcag cgcgagcacc gaaaacacgt tggcgaagcc gccctcggtc gcatgcagca
     8221 ccgcgatgag cggcacggcc accgccgcca ccccgagcga caccacgtag cgcaccgcga
     8281 gcacgcgcgc gcggaactcg tcggcgcagt atcttccgac gatggcgtca ttgagcggaa
     8341 tttgaccgaa taccgcgagc atcatgagca ccgccgcgcc gagcatggcc cagccctgca
     8401 ggttcgcggc cagcgccagc aaggggatct gcgccagcgc cacgccgatc atcagccgcc
     8461 ggagttcgaa ccggtcaatg agcgcgccca tcgccacctg ggcgaacgcg gcgatcgtgt
     8521 acaccaccgc cacgagcgcg ccgatgccga agttggtctg cgtcagcgcg cgcaggcgct
     8581 cgtcgaacac cttcggcatc gacaccgtgg tggaattgaa gatgatgccg ccgcaggccg
     8641 tggcgacgag cagcacggtg aagatgcgcg ccatggtgcg tccgtcgacg tgaaagccta
     8701 tcgacttcga ggtcttcttc acccggcccg gatccttcac cagcgcgaga aagccggccc
     8761 ccgcggccac gcagatcagc ccggggacga ag
//
LOCUS       DBNHKE010000257        29567 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG04813
            SRR25158312_k127_423076, whole genome shotgun sequence.
ACCESSION   DBNHKE010000257 DBNHKE010000000
VERSION     DBNHKE010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414221
            Sequence Read Archive: SRR25158312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 29567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:28:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,482
            CDSs (total)                      :: 3,437
            Genes (coding)                    :: 3,397
            CDSs (with protein)               :: 3,397
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 3 of 40
            Pseudo Genes (incomplete)         :: 36 of 40
            Pseudo Genes (internal stop)      :: 2 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158312_k127_423076"
                     /isolate="MAG04813"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.28 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC16O9_17280"
     CDS             <1..602
                     /locus_tag="AC16O9_17280"
                     /inference="COORDINATES: protein motif:HMM:NF048738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase Cds6 family protein"
                     /protein_id="HIR6518404.1"
                     /translation="TDFYGAGAYPINYPNEWDRQRGRNGYGIWVHGTPPDTYSRPPRA
                     SDGCIVLANPDLLSVGRLMQVGMTPVIIADEIEWTDAGQLAAERSSLALALERWRADW
                     ESRDTERYLTHYADEFRAGRQDRKTWAAHKRSVNGAKSWIRVALDKVSMLRYPRESDV
                     VVVTFEQDYRSSNLSNTMRKLQYWKKANGRWRILYEGAA"
     gene            599..1198
                     /locus_tag="AC16O9_17285"
     CDS             599..1198
                     /locus_tag="AC16O9_17285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6518405.1"
                     /translation="MKFRLSSLWKPLVVLSAAAAGPGALAAEPQVDLKTSAGTIRLEL
                     YPAKAPKTVANFLLYVKDGHFDGTIFHRVIPGFMIQGGGFDPGFKQKATRAPVVNEAQ
                     SAVKAGLKNEVGTVAMARTSDPNSATAQFFINVNDNAFLNWGDPRSDGNGYAVFGKVV
                     SGMDVVTKIAKTPTGPGGPFRSDVPREAVVIQSATVVGK"
     gene            1208..1942
                     /locus_tag="AC16O9_17290"
     CDS             1208..1942
                     /locus_tag="AC16O9_17290"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR6518406.1"
                     /translation="MPATLFISDLHLSGERPATNERFFEFLRTQASHAAALYVLGDLF
                     EYWIGDDELAAADGDPLARRVADALGEVSHRGVSVAFMHGNRDFLVGQRFGEASGARL
                     LEDPSVEEIGGVRTLLMHGDTLCTDDHDYQAWRRTARSSEWQRQFLAKTLPERRAALQ
                     ALREKSKEVVGAKPAEIMDVNDTAVRGALRAHDLTRLVHGHTHRAARHALEVDGRRCE
                     RWVLPDWYGRGGYLAVDDAGPRLMGF"
     gene            complement(1939..3111)
                     /locus_tag="AC16O9_17295"
     CDS             complement(1939..3111)
                     /locus_tag="AC16O9_17295"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071269 - L-homocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HIR6518407.1"
                     /translation="MAKPFEQSTLGVRAGQRRTDFKEHSEALFLTSSYVFENAAQAAA
                     GFAGGGEDFVYSRYTNPTVTMMQDRLAALEGAQACVATASGMSAILATAMVLLKAGDH
                     IVSSNAIFGATVQLFSTLLPKFGIETTFVPLSRIDEWQRAVRPNTRLLFLESPSNPLG
                     EVADLGAVAAVARKAGATLAVDNCLCTPALQRPLALGADLVIHSATKYLDGQGRVLGG
                     AVAGAKGPVLDGLVGFLRTAGPSISPFNAWVILKGMETLEIRMLAQSERALAVAQWLE
                     GHPQVARVYYPGLPSHPQHTLAKRQQRAGGAIVSFDVKGGKEAAWKVIDSTQLISITG
                     NLGDVKTTITHPATTTHGRLTPEARAASGIGDGLIRLAVGLEGVEDLKADLARGLG"
     regulatory      complement(3143..3221)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(3231..4751)
                     /gene="purF"
                     /locus_tag="AC16O9_17300"
     CDS             complement(3231..4751)
                     /gene="purF"
                     /locus_tag="AC16O9_17300"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR6518408.1"
                     /translation="MCGILGIVARSPVNQLLYDGLLLLQHRGQDAAGIVTAERKTFHM
                     QKGNGQVRDVFRTRNMRSLPGNIGVAHCRYPTAGSAFNSAESQPFYVNSPFGIVLGHN
                     GNLTNADALKEEMFRQDLRHINTNSDSEVLLNVLAHELEKTSVKLRLDPQTIFRAVAN
                     VHRRCRGAYAVIAMIAGYGVLAFRDPYGIRPACIGYNETEHGTQYMVASESVAVDALG
                     FRLLRDIAPGEAVLIDEEGNFHSQQCAPSSSLNPCIFEYVYLARPDSVIDGASVYEAR
                     LAMGDKLAEKIRHSYRHLDIDVVIPIPDSSRPSALQLAQGLGLPYREGFVKNRYIGRT
                     FIMPGQAVRRRSVRQKLNTIGVEFKGRNVLLVDDSIVRGTTSSEIVQMAREGGARKVY
                     FASAAPPVRCPNVYGIDMPTRSELIAAHRSEEDVGREIGADALIFQELEALEDAVRQV
                     NPRLKTFETSCFDGRYITGDVTNDYLHAIEVRRDAARDSGEDGEGAQLDLNLVTAQ"
     gene            complement(4780..5271)
                     /locus_tag="AC16O9_17305"
     CDS             complement(4780..5271)
                     /locus_tag="AC16O9_17305"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR6518409.1"
                     /translation="MTWLDYLILAGFVVSIAWGAWRGLVREVISVAAWVFAFLGANYF
                     GSPLAEVMPESIPTPELRVLIAFVAVFVVVLAVCTLVGQFLRKLVKVAGLGELDHVLG
                     GVFGVARALVILLAFAIVAGLTAFPRQSAWRDSVCGPPMGRAALALRPWLPQALGQRL
                     RYH"
     gene            complement(5273..5845)
                     /locus_tag="AC16O9_17310"
     CDS             complement(5273..5845)
                     /locus_tag="AC16O9_17310"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6518410.1"
                     /translation="MAEPQDVTTLKRRGRRRLVGAIALVLVAVIVLPWIFDPQPRQTA
                     PVSVRIPNEDESAFKPKAVPKPAPPKDLPPQVAAKPEVPAPQAEAKPVAPPPPSKPAP
                     DASERARAEAALANAQYLFQVGAYAEPDDVMAKLKAAKIPYYTEPVATEKGRVTRVRA
                     GPFASREAAERALKRVKALGFKPGGVTTSP"
     gene            complement(5838..7124)
                     /gene="folC"
                     /locus_tag="AC16O9_17315"
     CDS             complement(5838..7124)
                     /gene="folC"
                     /locus_tag="AC16O9_17315"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR6518411.1"
                     /translation="MSRSLAEWLEFIGRQHPDAIALGLDRVREVLGRMDVRIACPVVT
                     VAGTNGKGSVCAFLEAVLHASGRRVGLYTSPHLLRYNERVRISMQEASDALLVEGFSA
                     VERARGAVPLTYFEYGTLGAFWLFAREAPDVLVLEVGLGGRLDAVNVLDADCAVLTNV
                     GLDHMAYLGHTREAIGHEKAGIFRAGRPAVIAEADPPASVLAAAERVGASLLRLGRDF
                     GYRVHPGQWDYWGPGTRRPALAYPALRGQRQLHNAAGALAALDTLRERLPVSMQDVRR
                     GLAEVVLPGRFQVLPGRPQLILDVAHNPAAAEALAENLGAAGYAAETFAVFGMLRDKD
                     IAGVAQALAPRVTRWHLCTLGGPRGTDAEALAQILSAAGVRAPMFKHASVAEALGAAR
                     GAAGENDKIVAFGSFLTVAEVMQSLAATRTGAGTHG"
     gene            complement(7121..7990)
                     /gene="accD"
                     /locus_tag="AC16O9_17320"
     CDS             complement(7121..7990)
                     /gene="accD"
                     /locus_tag="AC16O9_17320"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR6518412.1"
                     /translation="MSWLQKLLPPKIQRSSGPKRAVPEGLWTKCPACDAVLYSTDLEK
                     NLNVCPKCGFHQRIGARARLDALLDADGRFEIGAEVLPVDSLKFRDSRKYTERLLEAE
                     AESGESEALVVMQGAIRSLGVVVAAHEFEFMAGTMGSVLGERFVRGVRVAIEQKLPFV
                     VITASGGARMQEGLLSLMQMAKTTAALTDLAERRLPFVTVLTDPTTGGVSASYAMIGD
                     VVIAEPKSLVGFAGPRVIEQTVRQKLPEGFQRAEFLLEKGAVDMIVDRRQLRDKLATL
                     LALLQGIAVDPPS"
     gene            complement(7987..8799)
                     /gene="trpA"
                     /locus_tag="AC16O9_17325"
     CDS             complement(7987..8799)
                     /gene="trpA"
                     /locus_tag="AC16O9_17325"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR6518413.1"
                     /translation="MSRIQARFDALQQGGRKALIPYITAGDPSPALTVPLMAALVEAG
                     ADILELGVPFSDPMADGPVNQRSSERALRHGVGLGDVLGMVRDFRRKDATTPVVLMGY
                     ANPIEAMGVDRFVESAKQAGVDGVIVVDYPPEECTQFATLARASGIDPIFLLAPTSTQ
                     KRIEAVARVGSGYLYYVSLRGVTGAGHLDLDDVAVRIPKIRAATRLPIGVGFGIRDAA
                     SAQRVALVADAVIIGSRIIQEIEAAGPEQAVARVRSLLKPIREALDAQVVVQ"
     gene            complement(8802..9995)
                     /gene="trpB"
                     /locus_tag="AC16O9_17330"
     CDS             complement(8802..9995)
                     /gene="trpB"
                     /locus_tag="AC16O9_17330"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR6518414.1"
                     /translation="MHDLPDARGHFGPYGGVFVAETLTHALEELLAAYEAARRDPAFA
                     AEFHRDLKHYVGRPSPVYHAQRWSQMLGGAQIYLKREDLNHTGAHKINNTIGQALLAR
                     RMGKSRVIAETGAGMHGVAAATVAARYGMQCVVYMGAEDIKRQVQNVYRMKLLGASVV
                     PVESGSKTLKDALNEAMRDWVTNVDNTFYIIGTVAGPHPYPMMVRDFQSVIGEECKRQ
                     MPEIAGRQPDAVIACVGGGSNAMGIFYPYIETEGVRLIGVEAAGEGLATGRHAASLCA
                     GKPGVLHGNRTYLLQDANGQIIETHSVSAGLDYPGVGPEHAWLKDSGRAEYVAVTDAD
                     ALRAFSDLCHYEGIIPALESAHAVAHAAQLAPSMPKDRILLVNLSGRGDKDMQTVAER
                     SGLKL"
     gene            complement(9988..10608)
                     /locus_tag="AC16O9_17335"
     CDS             complement(9988..10608)
                     /locus_tag="AC16O9_17335"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR6518415.1"
                     /translation="MRTRIKICGITRPQDASDAAAAGADAIGLVFYPPSPRFLSVERA
                     REIRDALPPFVQAVALFVNADAAQVAQVIGRVHPAMLQFHGEETPDFCGQFGLPYIKA
                     CRVSPGVDLLEYLRPFSAARGWLLDAHVEEYGGVGASFDWSLVPAKLARPLVLSGGLT
                     RDNVAAAVRRVRPWAVDVSSGVETAKGIKDAAKIAALIAEVRNADA"
     gene            complement(10605..11396)
                     /gene="truA"
                     /locus_tag="AC16O9_17340"
     CDS             complement(10605..11396)
                     /gene="truA"
                     /locus_tag="AC16O9_17340"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR6518416.1"
                     /translation="MRIALALEYDGSRFLGWQTQPGGGTVQDTLQAALSGIAGAPVQV
                     TCAGRTDRGVHARGQVVHFDTEAERPDSAWVRGVNALLPDSVAVLWAIRVADEFHARY
                     AARARTYRYVLLNRPVRPALAARHAGWYHTTLNIDAMRAAARQLVGEHDFSAFRSAEC
                     QAKSPVRTLHALEVEARGERIDFLLRANAYLHHMVRNIVGTLIYVGNGRHAPAWVGEV
                     LASRDRARAAPTFAAEGLYLERVEYAERWGLPARSRDGQGIPALP"
     gene            complement(11438..14170)
                     /locus_tag="AC16O9_17345"
     CDS             complement(11438..14170)
                     /locus_tag="AC16O9_17345"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="motility hub landmark protein FimV"
                     /protein_id="HIR6518417.1"
                     /translation="MVRVHKPVALLRLALAAMLLLPLLAQAAGLGRLSVKSFLGQPLR
                     AEIEIVAVQPGEESSLEARLASGNAFAQAGIEFNPALSGIQFAIDKRSDGRAVILVNT
                     RQPVNEPFVDLLVELRWSTGLFVREYTVLLDPPGYEGPKAAPMAAAPVSTPSASPAAP
                     PAPTTPPPGVLTAPKPEPSAAGTPMAAAPAAEPQSAPAAEPQPAPAEEPKPAPAPSPA
                     PALAAPVAARAAPGEQTYEVKKGDTLGEIALQYRPQGVTFHQMLIALQRANEDAFINN
                     NINLVRAGRILNIPDAEAAAAVDVQDANRLVIAQHADFAEYRSRLGITVAAAPAAPQA
                     AERSATGEITPQTEAPKPAPPKDQLKLSKADPNKPGAPAAKAAREDDLAARERALQEA
                     QSRVGELERNVSDLRKLLEIRNQQLKQLQDAAAPKPAPSPVSKAPEPPKPAPAPQAAT
                     APEPKPAAPSPAPVVAETPKPAAPEAPKPVAEAPKPAPPPAPVAAKPKPRPMAPPPLP
                     EPSLLDEFLGNPVALGGLGGVVLLLAGYGAYAWQRKKKSASKFQDSVLSVGSIEPTVG
                     KIDAGAAAPAAAAASVQPSSASQAPAGMATDDVDPIAEADVYMAYGRDAQAEEILKEA
                     LQKDPNRVPVMAKLLEIYANRRDAHSFEQIAVKLKGATNGAGPDWDKAASLGRNVDPG
                     NGLYGGGAAPAAAAAFAATAPAMAATPSVDFDIGGGTGSQPVPEPTPALDLDLGGPSA
                     APEQTDFAPSGTLVLDSQESKAASAGLDFDLGGGGEKPADTGGGLNFEFPGTAPAAPE
                     PTATPAAPSGSGGGLDFDLNLGSGGGETKEASAPLDLSAISLDLGTPGEAAAPAGDAQ
                     ADPKWQEVATKLDLAKAYEEMGDKDGARELLNEVLREGDAAQQGQANQLLAKLG"
     gene            complement(14240..15364)
                     /gene="asd"
                     /locus_tag="AC16O9_17350"
     CDS             complement(14240..15364)
                     /gene="asd"
                     /locus_tag="AC16O9_17350"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6518418.1"
                     /translation="MPRVGIVGWRGMVGSVLVQRMREERDFDLIEPVFFSTSQAGGKG
                     PSIGKDTGPVQSATDISALKSLPVIIACQGGDYTNEVYPQLRSAGWKGYWIDAASALR
                     MKGDATIILDPVNMPVIERALADGKRDFIGGNCTVSLMMMGMAGLFRRDLVEWVTSMT
                     YQAASGAGAANMRELAEQMAHVGDVAKPALADPGATALDIERRVSDALRNGAMPKEHF
                     GYPLAGSLLAWIDKDLGNGQSREEWKAQAEANKILGRSERPIPVDGICVRVGAMRCHS
                     QALTIKLKKDVPLDEVQGIVAEGNEWVRVVPNRREETLAELTPAAVTGKLAIPVGRLR
                     KLPMGGDYLAAFTVGDQLLWGAAEPLRRMLRILLDSGVRP"
     gene            complement(15377..16432)
                     /gene="leuB"
                     /locus_tag="AC16O9_17355"
     CDS             complement(15377..16432)
                     /gene="leuB"
                     /locus_tag="AC16O9_17355"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR6518419.1"
                     /translation="MRIAVLPGDGIGKEIVAQAVRVLRRLELPLELDEAPVGGAGYEA
                     AGDPLPEATLELARKADAILFGAIGGPQYDTLPRDKRPERGLLRLRKELGLFANLRPA
                     QVFPELAQASSLKPELVAGLDVLIVRELTGDIYFGEPRGITTLAGGEREGVNTMRYRE
                     SEIRRIARVAFEAARRRSKRLCSVDKANVLETTELWKQVVSELGKEYPDVQLSHMYVD
                     NCAMQLVRAPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDEKGKGLYEPIHG
                     SAPDIAGKGLANPLATMLSAAMMLRYSLGRAADADRIEAAVRKVLGQGLRTADIYTDG
                     CRKVGTQEMGDAVVAAL"
     gene            complement(16437..17078)
                     /gene="leuD"
                     /locus_tag="AC16O9_17360"
     CDS             complement(16437..17078)
                     /gene="leuD"
                     /locus_tag="AC16O9_17360"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6518420.1"
                     /translation="MDRFTVHTGIVVPLDRGNVDTDQIIPKQFLKSIKKTGYGPNLFD
                     AWRYMDHGEPGMDHAGRPRNPNFVLNDPRYQEATILMARKNFGCGSSREHAPWALLDY
                     GFRCVIAPSFGDIFYNNSFKNGLLPVVLQEAEVDRLFYECASFPGFRLTVDLDAQTVS
                     TADRSQVMRFEVDAFRKYCLLNGLDEIGLTLRHADKIRTFEAKHLAQHPWLAR"
     gene            complement(17085..18500)
                     /gene="leuC"
                     /locus_tag="AC16O9_17365"
     CDS             complement(17085..18500)
                     /gene="leuC"
                     /locus_tag="AC16O9_17365"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6518421.1"
                     /translation="MDRTLYDKLWDSHLVHSEADGTALIYIDRHLVHEVTSPQAFEGL
                     KLAGRKPWRMQSVVATADHNTPTTGWERGIAGIVDPTSRIQVETLDANMREHPAKAYF
                     PFLDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVLATQ
                     CLMTKKMKNMRVSVEGTLARGVTGKDIVLAVIGRIGTAGGNGHAMEFAGSAIRALSME
                     GRMTVCNMSIEAGARAGMVAVDEATIAYLKGRPFAPTGPDWDVAVAYWKTLKSDPNAK
                     FDREITLRAEEILPQVSWGTSPEMVAPVDGEVPDPEREKDAIKREGMERALQYMGLKP
                     RTRITDIKLDKIFIGSCTNSRIEDLRIAAAVVKGRRVAPSVRLAMVVPGSGLVKAQAE
                     KEGLDKVFRDAGFDWRQPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGDGGRTHLV
                     SPAMAAAAAVAGHFVDIRQWR"
     gene            complement(18562..19371)
                     /locus_tag="AC16O9_17370"
     CDS             complement(18562..19371)
                     /locus_tag="AC16O9_17370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6518422.1"
                     /translation="MRSFRVLALLLAAAAGCQTVQTTQPGAVGVDRQQSMLLSSKEVN
                     QSAASAYRQVLANASKKDQLNRDAALLARVRGVSQRLIPQTGVFRPDAPGWDWEVNLI
                     SSKELNAWCMPGGKIAFYTGLIEKLKLTDDEIAAVMGHEIAHALREHGRERASKAMAQ
                     GIALGILGAVAGVSRGGMDLTQLVLNVTLNLPNSRENETEADRIGVELAARAGYDPRA
                     AVAVWEKMARASEGQSLEILSTHPAPATRIADLKVYAARVMPLYEQARPRS"
     gene            complement(19381..20517)
                     /locus_tag="AC16O9_17375"
     CDS             complement(19381..20517)
                     /locus_tag="AC16O9_17375"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6518423.1"
                     /translation="MPPSLRLSAYFGAYFLFMGAYVPYFALYLAARGFGAGEIAFVMA
                     MPQLARIAAPSFWGWLADRSGAARAIVIGSGCAIVAGYLALGAAEGAIAVALVMLVMS
                     LASAGALPLVEAITMAAIEGRAERYGPIRMWGSIGFIAAVLGTGVWLDAHAPQSLLGV
                     ILVLAALALGTAFLLPRARPAAAGPGGAEARLGDVLRRADVIALLAACSCMSAAHGAL
                     YGFFSLHAEALGYSKTAIGTLWTLGVLAEIVVFLAWPRLARRYSLRVLLIASFLCAAA
                     RFVAIGWGTHVLALLVVAQLLHAATFGVFHAASVAAVHRLFTGRLQARGQALYSSLSY
                     GLGGGAGLLLAGWTWQTLGPGASFSVSAAFALAGGALVIWRVRV"
     gene            20560..23556
                     /locus_tag="AC16O9_17380"
     CDS             20560..23556
                     /locus_tag="AC16O9_17380"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013713.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermine/spermidine synthase domain-containing
                     protein"
                     /protein_id="HIR6518424.1"
                     /translation="MPGPAPPRAAFYLLFTLSGFAGLIYESIWTHYLKLYLGHAAYAQ
                     SLVLAVFMGGMALGAAGCARLSARLRNPLAAYALAEGVIGLAALGFHEAFVALTDWSF
                     AALLPALGAEWAVLGAKLALACLLILPQSVLLGATFPLMSAGLVRAHPSASGESVAML
                     YFTNSLGAAFGVLASGFVLIAWVGLPGTLRIAGVINLALAGIVWLLSRPLRAPALETR
                     HALETGVLTLLLAASFVTGVASFIYEISWIRMLSLVLGASTHSFELMLSTFILGLALG
                     GYAVRRRVDRVASPERLLGWVQVVMGLAALATLPVYDRSFELMERLMAGIARNDAGYA
                     LFNLSGQAIAALVMLPATFCAGMTLPLITGALLRQGAGEGAIGRVYAANTVGAIAGVL
                     FALHVGLPALGLKGALIAGALLDAALGLYLLYRFAAPRAHWVPVAAACAALFAAAALA
                     FELDANKMTAGVYRHGDLDSSRDATVLYAKDGKTATVHLVKYSSATSIRTNGKSDGSI
                     NLEGEAEADRGTDEITMVLTGALPLALKPEAKSAAVIGIGTGLTTHTLLQSLDLERVE
                     TVEIEAAMVQAARGFLPRNSAAFADPRGTIVIDDAKTYFSARQRRYDIIVSEPSNPWV
                     SGVSSLFTREFYRRIASHLAPGGLLVQWFQLYEIDPGLVASVLGALGAEFAHYAVYAP
                     SNHDVLIVASAAPLPPAPQARVFEHPGLAKELWTVHMLSAGDLDARYLGGRATLEPLF
                     ESYAARANSDYFPVLDLNAVRHRFAERSATGIVAITSAAVPVLDLLEPQFVRRPPNPL
                     HKGAYAFERIEKTRQARYARDFLVHLAPPVPEGIPPQLQKDLEVVQLRLLGCREARRF
                     DVWLHSLLRVAELVNGVLPAGEAAALWSRIGATSCLSTLHDFQRRWIALFTAVGARDA
                     RAMASLASALLEGTGNLGADAREYLLLAGMSGAIASGRPEQALALWQSQAPLLRGALA
                     QPVFRLLRCHAERAGCAAAFVAYNED"
     gene            complement(23553..24911)
                     /gene="der"
                     /locus_tag="AC16O9_17385"
     CDS             complement(23553..24911)
                     /gene="der"
                     /locus_tag="AC16O9_17385"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6518425.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRRRDAIVIDVPGVTRDRHYGE
                     GRVGARPFIVIDTGGLEPGAKSGIFAEMGRQAEQAMAEADAVVFLTDGRAGLHADDRE
                     IAARLRRIGARVWLVANKCEGMASDSAVAEFHALGLGQPNAISAAHGEGVADLIEGVL
                     AGFPEAAAEDEEARDKHPRVAVVGRPNVGKSTLVNALVGEERVIAFGEPGTTRDAIEV
                     PFERGGRRYTLIDTAGLRKRGRQGETVEKFSIVKTPQAIEQSNVAILVLDAVEGVSEQ
                     DAHVAGYILERARALVVAVNKWDAAGKEARERVKHELAWKLGFLGFAETHFLSAKAGK
                     GLGELMRSVDAAYAAAMAKMPTPKLTRALIAAVERQAPPRKGLRRPKLRYAHQGGHNP
                     PRIIIHGNSLDQLPESYRRYLEGVFRDTFALRGTPMRVEFRTGRNPYAGRGPAPRRSK
                     AP"
     gene            complement(24925..26049)
                     /gene="bamB"
                     /locus_tag="AC16O9_17390"
                     /gene_synonym="yfgL"
     CDS             complement(24925..26049)
                     /gene="bamB"
                     /locus_tag="AC16O9_17390"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR6518426.1"
                     /translation="MARRAALALLLPALSGCFGGSSDGPQPAALEPLAQPQALRVLWS
                     ANVGAAAAFEFSPALAGDALYAAARDGTVLRLDAASGRERWRVRVEAPLSGGVGADGT
                     TLAVATESGAVFALDALTGKERWRARVSSEVLAAPRLAAGMVLVRSADSRIHALDAAD
                     GKRRWTYQRAPAPLRLRTPQGMTPHADLLYAGFSGGKLVALALAGGTLRWEATVADPK
                     GTTELERVTDVVGEPAVVGREVCAAAFQGRVACFDAQNGNLLWARDISSVTGVSADAR
                     YAYLSDERGAVHALDRTNGRSLWKQDKLAQRQLSLPLPLGESVVLGDLEGFVHVLARD
                     SGAFVARLGTDGSPVRAAPLALSRGFLVQTQGGGLFALSP"
     gene            complement(26052..26684)
                     /locus_tag="AC16O9_17395"
     CDS             complement(26052..26684)
                     /locus_tag="AC16O9_17395"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR6518427.1"
                     /translation="MALQNLDLEEQEKLDALKAWWKDNARLIVLVVAAAAIAVTGTNG
                     WRWYQRQQALEAGALYATLAKAVRAGDAKALRDAGGALTERFAGTLYASMGALAAADF
                     HFKRGELKSAKAQLQWVVDRSSSEDFRDVARLRLAALLLDERAYDEALALLAAPPLEP
                     YAPQFAALRGDVLVAKGQPAEARAAYQLALEKAGGPLRDSVQMRLDALGG"
     gene            complement(26687..27949)
                     /gene="hisS"
                     /locus_tag="AC16O9_17400"
     CDS             complement(26687..27949)
                     /gene="hisS"
                     /locus_tag="AC16O9_17400"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR6518428.1"
                     /translation="MSTPLQAVRGMNDVLPDAAEQRLWLEGVVARWFARYGYRHLRTP
                     LLEHTALFRRAIGEATDIVEKEMYTFTDALNDESLTLRPEATASTVRAAIEHSLLYEG
                     PKRLWYQGPMYRHERPQKGRYREFHQFGAEALGFAGPDVDAELIVMCAALWRELGLDG
                     IRLELNTIGSSEERGRYRTDLIRHLEAHASALDADAKRRLHTNPLRVLDSKNPMVQQV
                     VASAPALMDYLGETSLAHFEGLKGLLATAGVAFTVNTKLVRGLDYYNLTVFEWVSDRL
                     GAQGTVCGGGRYDGLFAQLGGKPAPGVGFAAGIERLVALLGAPAPVAPGAYLVHQGEG
                     TLAYAFALGEQLRAAGFAVVLHAGGGSFKSQMKKADASGASLALIVGEDELKAGAVTV
                     KPLRDARPQQQVARAALASRLHELLKGS"
     gene            complement(27953..29209)
                     /gene="ispG"
                     /locus_tag="AC16O9_17405"
                     /gene_synonym="gcpE"
     CDS             complement(27953..29209)
                     /gene="ispG"
                     /locus_tag="AC16O9_17405"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR6518429.1"
                     /translation="MLERRFSSRVMVGPVAIGGGAPVVVQSMTNTDTADAEATARQVA
                     ELAAAGSELVRITVNTPEAAAQVAPIRARLDALGCAVPLVGDFHYNGHKLLAQFPDCA
                     RALAKYRINPGNVGRGAKRDEQFATMIEAAVRYAKPVRIGVNWGSLDAELLARMMDEN
                     ARRRDPLEADAVMREAVIASALESARAAEELGLGPERIVISCKMSQVQDLIAVYRELA
                     RRCTYALHLGLTEAGMGSKGIVASSAALAVLLQEGIGDTIRISLTPDPGGERTREVLV
                     AQELLQTMGIRSFAPMVAACPGCGRTTSTYFQELAARIQAFLREQMPQWRARYPGVEK
                     MRVAVMGCVVNGPGESKHADIGISLPGTNETPVAPVYVDGAKTVTLKGARIAEEFQAI
                     VEDYVRRNYAPGASRPRDRSIPIKVA"
     gene            complement(29211..>29567)
                     /locus_tag="AC16O9_17410"
     CDS             complement(29211..>29567)
                     /locus_tag="AC16O9_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HIR6518430.1"
                     /translation="PARQETPARTELASAAPRASAAAQPAAAPVDEEARIASGAWRRI
                     VLRFERESWVEITSGTGKLLVSQLHAEGSERVVVGVPPFSVVIGNAQHVQLVYNDQPV
                     DLKPHTKVEVARLTLD"
BASE COUNT         4356 a        10549 c        10082 g         4580 t
ORIGIN      
        1 tcaccgattt ctacggcgcg ggcgcctatc cgatcaatta cccgaacgag tgggaccggc
       61 agcgcggccg caacggctac ggcatctggg tgcacggcac gccgcccgat acctacagcc
      121 ggccgccgcg cgcaagcgac ggctgcatcg tgctcgccaa tcccgacctg ctgtcagtcg
      181 gacggctgat gcaggtcggc atgacgccgg tgatcatcgc cgacgagatc gagtggacgg
      241 acgccggtca gctggctgcc gagcgcagtt cgctggcgct tgccctggag cgctggcgcg
      301 ccgactggga aagccgcgat accgagcgtt atctcacgca ttacgcagac gagttccgcg
      361 ctggccgtca ggatcgcaag acctgggctg cgcacaagcg cagcgtcaac ggcgcaaagt
      421 cctggatccg tgtcgcgctc gacaaggtgt cgatgcttcg ctacccgcgc gagtccgacg
      481 tcgtggtcgt cactttcgag caggactacc gctcgagcaa cctttccaac accatgcgca
      541 agctgcagta ctggaagaag gcaaacggcc gctggcgcat cctttacgag ggcgccgcat
      601 gaaattcaga ctgtccagcc tgtggaaacc cctggtcgtg ctttcggccg cagcggccgg
      661 gcccggcgcg ctcgccgccg agccccaggt ggacctcaag accagcgccg gcaccatccg
      721 gctcgagctc taccccgcca aggcgccgaa gacggtggcc aacttcctgc tgtacgtgaa
      781 ggacggccat ttcgacggca ccatcttcca ccgcgtgatc ccgggcttca tgatccaggg
      841 cggcggcttc gatccgggct tcaagcagaa ggcgacgcgc gccccggtgg tcaatgaagc
      901 gcaaagcgcg gtgaaggccg ggctgaagaa cgaagtcggc accgtcgcca tggcgcgcac
      961 ctcggatccg aactcggcca ctgcgcagtt cttcatcaac gtgaacgaca acgccttcct
     1021 caactggggc gacccgcgca gcgacggcaa cggctacgcc gtgttcggca aggtggtctc
     1081 cgggatggac gtggtgacga agatcgccaa gacgccgacc ggcccgggcg gaccgttccg
     1141 cagcgacgtg ccgcgcgagg cggtggtgat ccagtcggcc accgtggtcg gcaagtagcc
     1201 cggcggcgtg cccgccacgc tgttcatctc ggacctgcat ctctcgggcg agcgcccggc
     1261 gaccaacgag cggttcttcg agttcctgcg cacgcaggcg tcgcacgcgg cagcgctgta
     1321 cgtgcttggc gacctgttcg agtactggat cggcgacgac gagctcgccg ccgcggacgg
     1381 cgacccgctg gcgcggcgcg tcgccgatgc gctcggagaa gtgtcgcacc ggggcgtgag
     1441 cgtggcgttc atgcacggca accgcgactt cctcgtcggc caacgttttg gcgaagcgag
     1501 cggtgcgcgc ctgctcgagg atccgtcggt ggaggagatc gggggcgtgc gtacgctgct
     1561 gatgcacggc gacaccctgt gcaccgacga tcacgactac caggcctggc gccgcaccgc
     1621 gcgctcgagc gagtggcagc gccagttcct cgccaagacc ctgcccgagc gacgcgccgc
     1681 gctgcaggcg ctgcgcgaga agagcaaaga ggtcgtcggc gcgaagccgg ccgagatcat
     1741 ggacgtgaac gacactgcag tgcgcggagc gctgcgtgcg cacgacctga cgcgccttgt
     1801 gcatggacac acgcaccgtg cggcgcgcca cgcgctggaa gtcgacggcc ggcgctgcga
     1861 gcgctgggtg ctgcccgact ggtacgggcg cggcggctac ctcgccgtcg atgacgccgg
     1921 tccgcgactg atgggcttct agcccaatcc gcgcgccagg tcggccttca ggtcctcgac
     1981 accctccagg cccaccgcga gccggatgag gccgtcgccg atacccgaag cggcgcgggc
     2041 ctccggtgtc aggcgcccgt gggtcgtcgt cgccgggtgc gtgatggtcg tcttcacgtc
     2101 gcccaggttg ccggtgatcg agatgagctg cgtcgagtcg atcaccttcc acgccgcctc
     2161 cttgccgcct ttcacgtcga aagagacgat ggcgccgccg gcgcgctgct ggcgctttgc
     2221 gagtgtgtgc tgcgggtggc tcggcaagcc cgggtagtag acacgggcca cctgcggatg
     2281 gccttcaagc cattgcgcca cggccagcgc gcgctccgac tgcgccagca tgcggatctc
     2341 cagcgtctcc atccccttga ggatgaccca ggcgttgaac ggcgagatcg acggcccggc
     2401 ggtacgcagg aaccccacca ggccgtccag cacgggcccc ttggcgccgg cgactgcacc
     2461 gccgagaacg cgcccctggc catcgagata cttcgtggcc gaatggatca ccaggtccgc
     2521 cccgagcgca agcgggcgct gcagcgccgg cgtgcacagg cagttgtcca cggccagggt
     2581 cgcccccgcc tttcttgcca ccgcggcgac ggcgccgagg tcggccacct cccccagcgg
     2641 gttggagggc gattccagga acaagagccg cgtgttcggc cgcaccgccc gctgccactc
     2701 gtcgatgcgg ctcagcggca cgaacgtcgt ttcgatgccg aacttcggca gtagcgtcga
     2761 gaacagctgc acggtcgcgc cgaagatggc gttcgaggac acgatgtggt cgcctgcctt
     2821 gagcaggacc atcgccgtgg cgaggatcgc cgacatgcca gacgcggtcg cgacgcacgc
     2881 ctgcgcgccc tcgagcgccg caagccggtc ctgcatcatc gtgacggtcg ggttggtgta
     2941 gcgcgagtag acgaagtcct cgccgccgcc ggcgaatccc gcagcggcct gcgccgcgtt
     3001 ctcgaacacg tagctcgagg tgaggaacag ggcctcggaa tgttccttga agtcggtgcg
     3061 gcgctggccg gcgcgtaccc ccagcgtgga ttgctcgaac ggctttgcca taaaaaaacc
     3121 cggtcctttc gttcactaag cggaccgggc ttcctcggca cgctttagct gtatttgtaa
     3181 cgcgcccgca agctgtggct caaatcggcg cgaaacgcac tatagcaaat tcattgcgcg
     3241 gtgaccagat tgaggtcgag ctgggcccct tcgccgtcct cgcccgagtc gcgcgcggcg
     3301 tcgcgccgca cctcgatggc gtgcaggtag tcgttggtga cgtcgccggt gatgtagcgc
     3361 ccgtcaaagc acgaggtctc gaacgtcttc agcctgggat tcacctggcg cacggcgtcc
     3421 tcaagggctt ccagctcctg aaagatgagg gcatcggcgc cgatctcgcg accgacgtcc
     3481 tcctcgctgc ggtgcgcggc gatcagctcg gagcgcgtcg gcatgtcgat gccgtacacg
     3541 ttgggacaac gcaccggcgg cgccgccgac gcgaagtaca ccttgcgtgc cccgccttcg
     3601 cgcgccatct gcacgatttc gctgctggtc gtgccgcgta cgatggagtc gtccaccagc
     3661 agcacgttgc gacccttgaa ctcgacgccg atggtgttca gcttctgccg caccgagcgc
     3721 cggcgcaccg cctgccccgg catgatgaag gtacggccga tgtagcggtt cttgacgaag
     3781 ccctcgcggt acggcagacc gagcccctgc gccagctgca gcgccgaggg ccggctcgaa
     3841 tccgggatcg ggattaccac gtcgatgtcg aggtggcggt agctgtggcg gattttctcc
     3901 gccagcttgt cgcccatcgc caggcgcgcc tcgtacaccg aggcgccgtc gatcacagag
     3961 tcgggccgcg ccaggtacac gtactcgaag atgcaagggt tgagcgagct cgacggcgcg
     4021 cactgctggc tgtggaaatt gccttcctcg tcgatcagca ccgcctcgcc cggcgcgatg
     4081 tcgcgcagca ggcgaaagcc gagcgcatcc acagccaccg actccgaggc caccatgtat
     4141 tgcgtgccgt gctcggtttc gttgtagccg atgcaggccg ggcggatgcc gtacggatcg
     4201 cggaaggcga gtaccccgta gcccgcgatc atcgcgatca cggcgtaggc accgcggcag
     4261 cggcggtgca cgttggccac cgcgcggaag atggtttgcg ggtccaggcg cagcttgacc
     4321 gaagtcttct ccagctcgtg cgccaggacg ttaagcagca cttcggaatc ggagtttgtg
     4381 ttgatgtgcc gcaggtcttg gcggaacatc tcctctttca gcgcatcggc attggtcagg
     4441 ttgccgttat ggccgagcac gatgccgaac ggcgaattga cgtaaaacgg ctgcgactcc
     4501 gcggaattga aggccgaccc tgcggtcgga tagcggcagt gcgccacgcc gatgttgccg
     4561 ggcagcgagc gcatgttgcg cgtgcggaaa acgtcgcgca cctggccgtt gcccttctgc
     4621 atgtggaagg tcttgcgctc ggcggtgacg atgcccgccg cgtcctggcc gcggtgctgc
     4681 aggagcagca gcccgtcata cagcagctgg ttgacgggcg agcgcgctac gatgccgagg
     4741 atcccgcaca tgcgagccgt ctcgcggtga cgttaagggt caatgatagc gcaggcgctg
     4801 gcccagcgcc tgcggcagcc acggccgcag tgccagcgcc gcgcgcccca tcggcgggcc
     4861 gcagaccgaa tcgcgccagg cgctctggcg cgggaatgcg gtaaggccgg caacgatggc
     4921 gaacgcgagc aagatgacaa gtgcccgcgc cacgccgaac acgcccccga gcacatggtc
     4981 caattcgccg agccccgcca ccttgacgag tttgcgcagg aactgcccca ccagcgtaca
     5041 aaccgccaga acgacgacga ataccgcgac gaatgcgatc agcacgcgca gctcgggcgt
     5101 cgggatcgat tcgggcatca cctcggccag cggactgccg aagtagttgg cgccgaggaa
     5161 cgcgaaaacc caggccgcga cggagatcac ctcgcgcacc aacccacgcc aggcgcccca
     5221 ggcgatcgac accacgaacc cggccaggat gaggtagtcg agccaggtca tttcacggac
     5281 tggtcgttac gccgcccggc ttgaacccca gggccttgac gcgcttcagc gccctttccg
     5341 cggcctcgcg actggcgaag ggaccggcgc gcacacgcgt caccctgccc ttttcggtcg
     5401 caaccggctc ggtgtagtag gggatcttcg ccgcctttag cttcgccatg acatcgtcgg
     5461 gttccgcgta cgccccgacc tgaaagaggt attgcgcatt ggcgagcgcc gcttcggcgc
     5521 gcgcgcgctc gctcgcgtca ggcgcgggtt tgctcggcgg cgggggagca accggcttgg
     5581 cttcggcctg cggcgccggc acctcgggct tcgccgcgac ttgcggcggg aggtccttgg
     5641 gcggcgcggg cttgggaacc gccttgggct tgaacgccga ttcgtcttcg ttcggaatgc
     5701 gcacgctcac gggcgcggtc tgccgcggct gcggatcgaa gatccagggc aggacgatca
     5761 ccgcgacgag caccagcgcg atagcgccta ccagccgccg ccggccgcgg cgcttcaggg
     5821 tcgtgacgtc ctggggttca gccatgggtg ccggcgccgg ttcgggtggc cgcgagggat
     5881 tgcatcactt cggcgacggt gagaaaggat ccgaaggcga cgattttatc attctctccc
     5941 gcggcccccc gggccgcgcc gagcgcctcg gcgacactgg cgtgcttgaa catcggcgcg
     6001 cggacccccg ctgcggacag gatctgcgcc agcgcctcgg cgtcggtacc gcgcggcccg
     6061 ccaagcgtgc acagatgcca gcgcgtgacg cgcggcgcca gcgcctgcgc aacgccggcg
     6121 atgtccttgt cccgcagcat cccgaacacg gcgaaggttt ccgcggcata gccggcggca
     6181 ccgagattct ccgccagcgc ctcggccgct gccgggttgt gcgcgacatc gaggatcagc
     6241 tggggcctgc ccgggagcac ctggaagcgc ccgggcagca ccacctcggc cagaccgcgg
     6301 cgcacgtcct gcatgctcac gggcaagcgc tcccgcaggg tgtcgagcgc cgcgagcgcg
     6361 cccgcggcgt tgtgcagctg tcgctgcccg cgcagcgccg gataggcaag cgcggggcgc
     6421 ctcgtccccg gcccccagta gtcccactgc ccgggatgca cgcgatagcc gaagtcccgc
     6481 cccaggcgta gcagcgacgc tccgacgcgc tcggccgcgg cgagcacgct cgcgggcgga
     6541 tccgcttcgg cgatgaccgc cggccgcccg gcacggaaga tgcccgcctt ctcgtggccg
     6601 atggcctcgc gcgtgtggcc caggtacgcc atgtggtcga gaccgacgtt ggtgagcact
     6661 gcgcaatcgg cgtcgagcac gttgaccgcg tcgaggcgtc cgcccaagcc cacctcgagc
     6721 accagcacgt cgggcgcctc gcgcgcgaac agccagaatg cccccagcgt gccgtactcg
     6781 aagtaggtga gcggcacggc gccgcgcgcc cgctccacgg cgctgaagcc ttcgacgagc
     6841 agcgcgtcgg aggcctcctg catgctgatg cgcacgcgct cgttgtagcg cagtaggtgc
     6901 ggcgaggtgt acagcccgac gcgccggccg gaagcgtgca ggacggcttc caggaatgcg
     6961 cagacggagc ccttgccgtt ggtaccggcg accgtcacga cgggacacgc gatgcgcaca
     7021 tccatgcgcc cgagcacctc gcgcacgcga tccagtccca gcgcgatggc gtccgggtgc
     7081 tggcgcccga tgaactccag ccactcggcg agcgagcggc tcacgaaggc gggtcgacgg
     7141 cgattccctg tagcagcgcg agcagcgtgg cgagcttgtc ccgcagctgg cggcggtcga
     7201 cgatcatgtc gaccgcgcct ttttcgagca ggaactccgc ccgctggaag ccttccggga
     7261 gcttctggcg cacggtctgc tcgatgacgc gcggccccgc gaagccgacc agggacttgg
     7321 gctcggcgat caccacgtcg ccgatcatgg cgtagctcgc cgacacaccg cccgtggtgg
     7381 ggtcggtgag caccgtcacg aacggcaggc gacgctcggc gagatcggtg agcgccgcgg
     7441 tggtcttcgc catctgcatg agcgagagca agccctcctg catgcgcgcc ccgccgctcg
     7501 cggtgatgac gacgaacggc agcttctgct cgatcgccac gcgcacgccg cgtacgaaac
     7561 gctcgccgag caccgagccc atggtgccgg ccatgaactc gaactcgtgc gccgccacca
     7621 ccacgccgag cgacctgatc gcgccctgca tcaccaccag tgcctcggac tcgcccgact
     7681 cggcctccgc ctccagcagc cgctcggtgt atttgcgcga atcgcggaac ttcaggctgt
     7741 ccaccggcag cacctcggcg ccgatctcga aacgcccgtc ggcgtcgagc agcgcgtcga
     7801 gccgcgcgcg cgcgccgatt ctctggtgaa agccgcactt ggggcataca ttgaggttct
     7861 tctccaggtc ggtgctgtag agcaccgcat cgcaggccgg gcacttggtc cacaggcctt
     7921 cgggcacggc gcgcttcggc cccgaagagc gctggatctt cggcggcagg agcttttgca
     7981 gccagctcat tgcacgacta cctgcgcgtc gagcgcttcg cggatcggct tgagcagcga
     8041 tcttacgcgg gcgacggcct gctccggccc ggccgcctcg atctcctgga tgatgcggct
     8101 gccgatgatc accgcgtccg cgactagcgc cacgcgctgc gcggaggcgg cgtcgcggat
     8161 gccgaagccg acgccgatcg gcaggcgcgt cgccgcccgg atcttcggaa tgcgaacggc
     8221 cacgtcgtcc agatccaggt gccccgcgcc ggtcacgccg cgcagcgata cgtaatacag
     8281 gtaaccgctg ccgacccgcg ccaccgcttc gatgcgtttc tgggtggagg tcggcgccag
     8341 caggaagatc ggatcgatgc cgctcgccct ggcgagcgtc gcaaactgcg tgcactcctc
     8401 aggcgggtag tcgacgacga tgacgccgtc cacgcccgcc tgcttcgcgg actcgacgaa
     8461 gcgatccacg cccatcgcct cgatcgggtt ggcgtagccc atcagcacca ccggcgtcgt
     8521 cgcatccttg cggcggaaat cgcgcaccat gccgagtacg tcgccgagac ccacgccgtg
     8581 gcgcagcgcc cgctcgctgg aacgctggtt caccgggccg tcggccatcg gatcgctgaa
     8641 cggcacgccc agctcgagga tatcggcgcc cgcttcgacc agcgccgcca tcagcggcac
     8701 ggtcagcgcc ggtgacggat cgcccgcagt gatgtacgga atgagcgcct tgcgcccccc
     8761 ctgctgcagg gcatcgaagc gggcctggat gcgcgacacg gctagagctt gagccccgag
     8821 cgctcggcca cggtctgcat gtccttgtcg ccgcggccgg aaagattgac gagcaggatg
     8881 cggtccttcg gcatcgaggg cgcgagttgc gctgcatgcg cgacggcatg cgccgactcg
     8941 agcgcgggga tgatgccttc gtagtggcac aggtcgctga acgcgcgcaa cgcgtccgcg
     9001 tcggtgacgg cgacgtactc ggcgcggccc gagtccttca gccaggcgtg ctcgggcccg
     9061 acgcccggat agtcgagtcc cgcggaaacc gagtgcgtct cgatgatttg gccgttggca
     9121 tcctgcaaga ggtaggtgcg gttcccgtgc agcacgccgg gcttgccggc gcagagcgac
     9181 gccgcgtgcc ggccggtggc gaggccctcg cccgcggcct cgacgccgat caggcgcacg
     9241 ccctcggtct cgatataggg atagaagatg cccatggcgt tggagccgcc gccgacgcac
     9301 gcgatcaccg cgtcgggctg gcggccggcg atctcgggca tctggcgctt gcactcctcg
     9361 ccgatcaccg attggaagtc gcgcaccatc atcggatagg gatgcgggcc ggccaccgtg
     9421 ccgatgatgt agaaggtgtt gtcgacattc gtgacccagt cgcgcatcgc ctcgttgaga
     9481 gcgtccttga gcgttttcga accggattcg accggcacca cgctcgcgcc gagcagcttc
     9541 atgcggtaca cgttctgcac ctggcgcttg atgtcctcgg cacccatgta gaccacgcac
     9601 tgcatgccat agcgcgcggc caccgtagcg gcggccacgc cgtgcatgcc ggcaccggtc
     9661 tcggcgatca cacgcgactt gcccatgcgc cgcgcgagca gcgcctggcc aatggtgttg
     9721 ttgattttgt gcgcgccggt gtggttgagg tcctcgcgct tgaggtagat ctgcgccccg
     9781 cccagcatct gcgaccagcg ctgcgcgtgg tacaccgggc tcgggcgccc gacataatgc
     9841 ttgaggtcgc ggtgaaactc ggctgcgaac gcgggatcgc gccgcgccgc ctcataggcg
     9901 gcgagcagtt cctccagcgc gtgagtcagc gtctccgcga cgaacacgcc gccgtagggg
     9961 ccgaagtgcc cgcgcgcgtc gggcaagtca tgcatctgca ttcctgacct ccgcgatgag
    10021 cgccgcgatc ttcgcggcgt ccttgatgcc cttggccgtt tcgacgccgc tggacacgtc
    10081 caccgcccag ggccgcacgc gccgcacggc cgccgcgacg ttgtcgcgcg tcagaccgcc
    10141 cgagagcacc agcggccgcg ccagcttggc gggcacgagc gaccagtcga agctcgcgcc
    10201 gacgccgccg tattcctcga cgtgcgcgtc gagcagccag ccgcgcgccg cggaaaacgg
    10261 gcgcaggtat tctagcaaat cgacccccgg cgacacgcgg cacgccttga tgtacggcag
    10321 cccgaactgg ccgcagaaat cgggcgtttc ctcgccgtgg aattgcagca tcgcggggtg
    10381 cacccggccg atcacctgcg cgacctgcgc cgcgtcggcg ttgacgaaga gcgcaacagc
    10441 ctgcacgaac ggcggcagcg catcgcggat ctcccgcgcg cgctccacgc tcaggaatct
    10501 ggggctcggg ggatagaaca ccaggccgat cgcatcggcg cccgcggcgg ccgcgtccga
    10561 agcgtcctgg ggtcgcgtga tcccgcagat tttgatgcgc gtcctcacgg cagggccggg
    10621 atcccctgtc cgtcgcgcga gcgcgccggc aagccccagc gttcggcgta ctccacacgc
    10681 tcaaggtaca ggccctcggc ggcgaaagtc ggcgccgcgc gggcgcggtc ccgcgacgcc
    10741 agcacctcgc ccacccaggc cggcgcgtgc cggccgttgc cgacgtagat cagcgttccg
    10801 acgatgttgc gcaccatatg atgcaggtag gcgttcgcac gcaggagaaa atcgatgcgt
    10861 tcgccgcgcg cctcaacctc gagcgcatgc aaggtgcgca ctggcgactt ggcctggcac
    10921 tcggccgagc gaaacgccga aaagtcgtgc tcgccgacca gctggcgcgc ggcggcgcgc
    10981 atcgcgtcga tattcagcgt ggtgtggtac cacccggcgt ggcgcgcggc gagcgccggg
    11041 cgcaccggcc ggttgagcag cacgtagcga taagtgcggg cgcgcgcagc gtaacgcgcg
    11101 tggaactcgt cggccacgcg tatcgcccac aataccgcga cggagtcggg cagcagcgcg
    11161 ttaacgccgc gcacccacgc gctatccgga cgttccgcct cggtatcgaa atgcaccacc
    11221 tggccgcggg cgtgcacgcc gcgatcggtg cggccggcgc aggtgacttg cacgggggcg
    11281 ccggcgatgc ccgagagcgc ggcctgcagc gtgtcctgca ccgtgccgcc gccgggctgc
    11341 gtctgccagc cgaggaagcg gctaccgtcg tattccagtg cgagcgcgat tctcatgcgc
    11401 gggagaatga acccggcgcc tcgcgcgccg tcgtgcgtca gcccagcttg gccagcagct
    11461 ggttggcctg cccctgctgc gccgcgtcgc cttcgcgcag cacctcgttg agcagctcgc
    11521 gcgcgccgtc cttgtcgccc atttcttcgt aggccttggc caggtcgagc ttggtggcga
    11581 cttcctgcca cttgggatcc gcctgcgcgt cgcctgcggg tgcagccgcc tcgcccggtg
    11641 tgccgagatc caggctgatc gcggacaggt ccaagggggc cgaggcctct ttggtttcgc
    11701 ctccgccact gcccaggttc aggtcgaagt ccaagccacc gccggagccc gaaggcgcag
    11761 ctggcgtggc tgtcggctcg ggcgcagcag gcgctgtgcc aggaaactcg aagtttaggc
    11821 cgccgccggt gtcggccggc ttctcgccgc cgccgcccaa gtcgaagtcg agcccggccg
    11881 aggccgcctt ggattcctgc gaatcgagga ccagcgtgcc gctaggcgca aaatctgtct
    11941 gctcgggcgc cgccgacggg ccgccgagat cgaggtcgag cgcaggcgta ggctccggca
    12001 cgggctgcga gcccgtgccg ccgccgatgt cgaagtccac ggacggcgtc gcggccattg
    12061 ccggtgccgt cgccgcaaag gccgccgcgg ccgccggcgc ggcgccaccg ccgtacaggc
    12121 cgttgcccgg atcgacgtta cgacccagcg acgccgcctt gtcccaatcg ggtcccgcgc
    12181 cgttcgtagc gcctttcagc ttgacggcga tctgctcgaa agagtgcgca tcgcgacggt
    12241 tggcgtagat ctctagcagc ttggccatta ccggcacccg gttcggatcc ttttgcagcg
    12301 cttccttcag gatctcctcg gcctgagcgt cgcgcccgta ggccatgtag acgtcggcct
    12361 cggcgatcgg atcgacgtcg tcagtcgcca tgccggccgg cgcctggctg gccgaggacg
    12421 gctgcacgct cgccgccgcc gccgccgggg cagcggcgcc cgcatcaatc ttgcctaccg
    12481 tcggctcgat cgatccgacg ctgaggacgc tatcctggaa tttgctagcg gatttctttt
    12541 tacgttgcca ggcgtaggcg ccatagcccg cgagcagcag cacgacgccg cccaagccgc
    12601 ctagggccac ggggttgccg aggaactcgt cgagcaggct cggctcggga agcggcgggg
    12661 gcgccatcgg ccgcggctta ggcttggcgg caaccggtgc aggcggcggc gccggtttcg
    12721 gtgcctcggc taccggcttg ggtgcctcgg gcgcggcagg tttgggggtc tcggcgacta
    12781 cgggtgcggg gcttggagcg gcaggcttag gctccggcgc ggttgccgcc tgcggcgccg
    12841 gcgctggctt tggcggttcg ggcgccttgg agaccggcga cggcgcgggc ttcggtgccg
    12901 ccgcgtcctg caactgcttg agctgctggt tgcggatctc gagcagcttt cgaaggtcgc
    12961 tgacattgcg ttccagctcg ccgacccgag actgcgcctc ctgcagcgca cgctcgcgcg
    13021 ccgcgagatc gtcttcgcgc gcggccttgg cggccggcgc gcccggcttg ttcggatccg
    13081 ctttggacag cttcagctgg tccttgggcg gcgcgggctt gggggcctcg gtttgcggcg
    13141 taatctcgcc ggtggcgctg cgctcggcgg cttgcggcgc ggccggcgcg gcggcgactg
    13201 tgatgcccaa gcggctgcgg tactcggcaa agtcggcgtg ctgcgcgatc accaggcgat
    13261 tggcgtcctg aacgtcgacg gccgcggccg cctccgcatc gggaatgttg agaatgcgcc
    13321 cggcgcgcac gaggttgatg ttgttgttga tgaacgcatc ctcgtttgcg cgttgcagcg
    13381 cgatcagcat ctggtgaaac gtgacgcctt gcggccgata ctgcagcgcg atctcgccca
    13441 gtgtgtcgcc cttcttcact tcataggtct gctcgcctgg cgcggcccgc gccgcgacag
    13501 gcgctgcgag cgcgggcgcg ggcgacggtg ctggcgcggg cttcggttcc tcggccggcg
    13561 ccggctgcgg ctcggcagcc ggtgcagact gcggctcggc agcgggtgcg gcggccatcg
    13621 gcgttccggc ggcgctcggt tcgggcttgg gcgccgtgag cacgccaggc ggcggcgttg
    13681 tcggcgccgg aggcgccgcg ggcgatgccg acggcgtcga caccggagcg gcggccatcg
    13741 gtgccgcctt cggtccctcg tacccgggcg gatcgaggag cacggtgtac tcgcgcacga
    13801 acaacccggt cgaccagcgc agctccacca gcagatcgac gaacggctcg ttcaccggct
    13861 ggcgcgtatt gacgaggatg accgcgcggc cgtcgctgcg cttgtcgatc gcgaactgga
    13921 ttccgctcag cgcgggattg aactcgatgc cggcctgcgc gaacgcgttg cccgaagcca
    13981 gacgcgcctc gagcgaggat tcctcacccg gctgcaccgc gacgatctcg atctcggcgc
    14041 gcagcggctg ccccagaaag gacttcacgc tcaaccggcc gagacccgcc gcctgcgcaa
    14101 gcagaggcag caacaacatc gctgccaagg cgagtcgtag caacgcgacc ggtttatgca
    14161 cccgcaccac cctgagtctt tcttatagga tttcgtacca taacatcatg gcgttaggcc
    14221 tgcaagctga gacattttct taaggccgga cgcccgaatc caggaggatc cggagcatcc
    14281 ggcggagcgg ctccgcggcg ccccaaagca attggtcgcc gaccgtgaac gccgccagat
    14341 agtcgccgcc cataggcagt ttgcgcaggc ggccgaccgg aatcgcgagc ttgccggtca
    14401 ctgcggcagg cgtcagttcg gccagcgtct cttccctgcg gttggggacc acgcgcaccc
    14461 attcgttgcc ctccgccacg ataccctgca cttcgtcgag cggcacgtcc tttttgagct
    14521 tgatggtgag cgcctggctg tggcagcgca ttgcgcccac gcgcacgcag atgccatcca
    14581 ccgggatcgg ccgctcgctg cgcccgagga ttttgttcgc ctccgcctgg gccttccact
    14641 cctcgcggct ctggccgttg ccgaggtcct tgtcgatcca ggcgagtagg ctcccggcca
    14701 gcggataccc gaagtgctcc ttgggcatcg cgccattgcg cagcgcgtcg gaaacacgcc
    14761 gctcgatgtc gagtgccgtg gcgccgggat cggccagcgc gggcttggcg acgtctccga
    14821 cgtgcgccat ttgctcggcc agctcgcgca tgttcgcggc cccggcgccc gacgcggcct
    14881 gataggtcat gctggtcacc cactcgacga ggtcgcgccg gaacagcccg gccatgccca
    14941 tcatcatcag gctcacggtg cagttgccgc cgatgaaatc gcgcttgcca tcggcgagcg
    15001 cgcgctcgat caccggcatg ttcacgggat cgaggatgat cgtcgcgtcg cccttcatgc
    15061 gcagcgcgct cgccgcgtcg atccagtagc ccttccagcc ggccgagcgc aactgcgggt
    15121 agacctcgtt cgtgtaatcg cccccctggc aggcgatgat caccggcagg cttttcagcg
    15181 cggaaatgtc ggtcgcgctc tgcaccggcc cggtgtcctt gccgatcgac gggcccttcc
    15241 cgcccgcctg cgaggtggag aagaacaccg gctcgatgag gtcgaagtcg cgctcctcgc
    15301 gcatgcgctg cacgagcacc gacccgacca tgccccgcca cccgacgatt ccgaccctcg
    15361 gcattgccct taccccctac agcgccgcca ccacggcgtc gcccatttcc tgcgtgccga
    15421 ccttgcggca gccgtcagta tagatgtccg cggtgcgcag gccctgcccc agcaccttgc
    15481 gcaccgccgc ctcgatccgg tcggcgtccg ccgcacggcc gagcgagtag cgcagcatca
    15541 tcgccgctga cagcatcgtc gccaaggggt tggcgagccc cttgccggcg atatccggcg
    15601 ccgagccgtg gatcggctcg tagagcccct tgcctttctc gtccagggaa gccgagggca
    15661 gcatgccgat cgagccggtg agcatggagg cttcgtccga caggatgtcg ccgaacatgt
    15721 tgccggtcac gatgacgtcg aactgcttgg gcgcgcgcac gagctgcatg gcgcagttat
    15781 cgacgtacat gtggctgagc tgaacgtccg ggtactcctt gccgagctcg cttaccacct
    15841 gcttccacag ctcggtggtc tcgagcacgt tcgccttgtc caccgagcac aggcgcttgc
    15901 tgcgccggcg cgccgcctcg aaggcaacgc gcgcgatgcg gcgaatctcg ctttcgcggt
    15961 agcgcatggt gttgacaccc tcgcgctcgc cgccggcgag cgtggtgatg ccgcgcggct
    16021 cgccgaagta gatgtcgccc gtcagctcgc gcacgatgag cacatcgagc ccggccacca
    16081 gctcgggctt gagcgaggag gcctgcgcca gttccggaaa cacctgcgcc gggcgcaggt
    16141 tggcgaacag gccgagctcc ttgcgcagcc gcagcaggcc acgctcgggc cgcttgtcgc
    16201 gcggcagcgt gtcgtactgc ggcccgccga tggcgccgaa caggatcgcg tccgctttgc
    16261 gcgcgagctc gagcgtcgcc tcgggcaacg ggtcgcccgc cgcctcgtag cccgcgccgc
    16321 ccaccggggc ctcgtcgagc tccaagggaa gctccaggcg acgcagcacc cgcaccgcct
    16381 gggcgacgat ctccttgccg atcccgtccc cgggcagtac cgcgatcctc atcgcgtcac
    16441 cgggcaagcc agggatgctg cgcgagatgc ttggcttcga aggttcggat cttgtcggcg
    16501 tggcgcagcg tgaggccgat ctcgtcgagg ccgttcagca ggcagtactt gcgaaacgca
    16561 tccacctcga agcgcatcac ctgcgagcga tccgccgtgg acaccgtctg tgcgtcgagg
    16621 tccaccgtaa ggcggaaacc cggaaacgac gcgcactcgt agaacagccg atcgacctcg
    16681 gcctcctgca gcacgaccgg cagcaggccg ttcttgaagc tgttgttgta gaagatgtcg
    16741 ccgaacgagg gcgcgatcac gcagcgaaag ccgtagtcga gcagcgccca gggcgcgtgc
    16801 tcgcgcgagc tgccgcagcc gaagttcttg cgcgccatga gaatcgtcgc ctcctgatag
    16861 cgcgggtcgt tcagcacgaa gttcgggttg cgcgggcgcc cggcgtggtc catgccgggc
    16921 tcgccgtggt ccatgtagcg ccacgcgtcg aacaggttcg gcccgtagcc ggtcttcttg
    16981 atcgacttca ggaactgctt ggggatgatc tggtcggtgt cgacgttgcc gcggtcgagc
    17041 ggcaccacga tcccggtgtg cacggtgaat ctatccataa ggccctagcg ccactgccgg
    17101 atgtcgacga aatggcccgc caccgcggcg gccgcggcca tcgccgggct caccaggtgc
    17161 gtgcggccgc cgtctccctg gcgcccttcg aagttgcggt tggaggtcga ggcgcagcgc
    17221 tcgccgggct cgagccggtc ggcgttcatc gcgaggcaca tcgagcagcc cggctggcgc
    17281 caatcgaagc ccgcgtcgcg gaacaccttg tcgaggcctt ccttctcggc ctgcgccttc
    17341 accagtccgg agcccggcac caccatggcg aggcgcacgc tcggcgccac gcgccggccc
    17401 ttgaccacgg ccgccgcgat gcgcaggtcc tcgatgcgcg agttggtgca cgagccgatg
    17461 aagatcttgt cgagcttgat gtcggtgatc ctggtgcgcg gcttgagccc catgtactgc
    17521 agcgcgcgct ccatgccctc gcgcttgatg gcgtccttct cgcgctcggg gtccggcact
    17581 tcgccgtcca ccggcgccac catctcgggc gaggtccccc aggacacctg cggcaggatt
    17641 tcttcggcgc gcaaggtgat ctcgcggtcg aacttcgcgt tggggtcgga cttcagcgtc
    17701 ttccagtagg cgaccgccac atcccagtcg ggacccgtgg gcgcgaacgg gcggcccttc
    17761 aggtaggcga tcgtcgcctc gtcgaccgcg accatgccgg cgcgcgcgcc ggcctcgatc
    17821 gacatgttgc acaccgtcat gcgcccttcc atcgagagcg cgcggatggc gctgccggcg
    17881 aactccatgg catggccgtt gccgccggcg gtgccgatgc gcccgatgac cgccagcacg
    17941 atgtccttgc cggtgacgcc gcgcgcaagc gtaccctcga cgctgacgcg catgttcttc
    18001 atcttcttgg tcatcaagca ctgcgtggcg agcacgtgct cgacttcgga agtgccgatg
    18061 ccgtgcgcca ggcaggcgaa ggcgccgtgc gtgctggtgt gcgaatcgcc gcacaccacg
    18121 gtcatgccgg gcagcgtcgc gccctgctcg ggcccgatga cgtgcacgat cccctggcgc
    18181 ttgtcgagga acgggaagta cgccttcgcg ggatgctcgc gcatgttggc gtcgagcgtt
    18241 tccacctgga tgcgcgaggt gggatcgacg atgccggcga tgccgcgctc ccagccggtg
    18301 gtgggggtgt tgtggtcggc ggtggcgacc accgactgca tgcgccaggg cttgcgcccg
    18361 gcgagcttca gtccctcgaa cgcctgcgga ctggtgactt catgcaccag gtgtcggtcg
    18421 atgtagatga gcgcggtgcc gtccgcctcg gagtgcacga ggtggctgtc ccagagcttg
    18481 tcgtagagcg tgcggtccat atgcggcgtc gcggaaacgc aggattatgg cacagcaaac
    18541 tccttgatag gactgggttt actaagagcg gggccgcgcc tgctcgtaga gcggcatgac
    18601 gcgcgcggca tacaccttca ggtcggcgat gcgcgtcgcg ggtgccgggt gcgtggacag
    18661 gatttcgagc gactggccct cgctcgcccg cgccatcttc tcccacaccg cgacggcggc
    18721 gcgcggatcg tagccggcgc gtgccgcgag ctccacgccg atgcggtcgg cctcggtctc
    18781 gttctcgcgc gagttcggca ggttgagcgt cacgttgagc accagctgcg tcaggtccat
    18841 gcctcctcgc gatacgcccg cgacagcgcc gagaatgccc agcgcgatgc cctgcgccat
    18901 cgccttcgag gcgcgctcgc gcccgtgctc gcgcagcgca tgcgcgatct cgtgccccat
    18961 cacggcagcg atctcgtcgt cggtgagctt cagcttttcg atcagcccgg tgtagaacgc
    19021 gatcttgccg cccggcatgc accaggcgtt cagctccttc gagctgatca ggttcacctc
    19081 ccagtcccag cccggcgcgt cgggccggaa cacgccggtc tgaggaatga gtcgctgcga
    19141 gacgccgcgc acgcgcgcca gcaatgccgc gtcccggttc agctggtcct tcttcgaggc
    19201 gttcgccagc acctgccggt aggcgctcgc ggcactctgg ttgacctctt tcgaggacag
    19261 cagcatcgac tgctgccggt cgactccgac cgccccgggt tgcgtggttt gcaccgtctg
    19321 gcagccggct gcggccgcca gcaacagcgc gaggacacgg aagctccgca tggcaaaagc
    19381 ctacacgcgc acgcgccaga tgacgagcgc cccgcccgcc agcgcgaacg cagcgctcac
    19441 cgagaagctc gccccggggc cgagcgtttg ccaggtccag cccgcaagga gcagcccggc
    19501 gccgccgccc agcccgtagg acaggctcga atacagcgcc tgcccccgcg cctgcagccg
    19561 accggtgaac aggcggtgca ccgcggccac cgaggcggcg tggaacacac cgaaggtcgc
    19621 ggcgtgcagg agttgcgcca caaccagcag cgccagcacg tgggtgcccc agccgatggc
    19681 cacaaagcgc gccgccgcgc acaggaagct cgcgatgagc agcacgcgca ggctgtagcg
    19741 ccgggcaagc cgcggccacg ccaggaacac gacgatctcg gccagtacgc cgagcgtcca
    19801 cagcgtcccg atcgcggtct tgctgtagcc cagcgcctcg gcatgcagcg agaaaaagcc
    19861 gtacagcgcg ccgtgcgccg cgctcatgca actgcaggcg gcaagcaagg caatcacatc
    19921 ggcacggcgc agcacgtcgc caaggcgcgc ttcggcgccg ccggggccag ccgccgcggg
    19981 ccgcgcacgg ggcagtagga acgcggtgcc cagcgcaagc gcggccagca cgaggatcac
    20041 gccgaggagc gattgcggcg cgtgggcgtc cagccacacc ccggtgccga gcacggcggc
    20101 gatgaagccg atcgagcccc acatgcggat cgggccgtag cgctccgcgc ggccctcgat
    20161 cgcggccatg gtgatcgcct ccacgagcgg cagtgccccg gccgaggcga ggctcatcac
    20221 gagcatgacg agggcgaccg cgatcgcccc ttcggccgcg ccgagcgcga ggtagccggc
    20281 gacgatggca catccggagc cgatcacgat ggcgcgggcg gcgccgctgc gatcggccag
    20341 ccagccccag aacgagggcg ccgcgatgcg cgcgagctgc ggcatcgcca tgacgaacgc
    20401 gatctccccg gcgccgaagc cgcgcgccgc gaggtacagc gcaaagtacg gcacgtaggc
    20461 gcccatgaac aggaagtacg cgccgaaata agcggacagt cggagcgacg gcggcatcgc
    20521 ggcgcatctt accggcacgg cgtgctataa ccgcccgcca tgccgggccc cgcaccgccg
    20581 cgtgccgcgt tctacctgtt gttcaccctg tccggattcg cgggactgat ctacgaatcc
    20641 atctggaccc attacctcaa gctatacctc gggcacgccg cctacgcgca gtcgctggtg
    20701 ctggcggtat tcatgggggg catggcgctc ggcgccgccg gctgcgcgcg cctgtcggcg
    20761 cgcctgcgca acccgctcgc cgcctacgcg ctcgcggaag gcgtgatcgg gctcgccgcg
    20821 ctgggtttcc atgaagcctt cgtcgcgctc accgactggt cgttcgcggc gctgctgccg
    20881 gccttgggcg ctgaatgggc ggtgctcggc gcgaagctcg cgctggcgtg ccttctgatc
    20941 ctgccgcaat cggtgctgct cggcgccacc tttccgttga tgagcgcggg cctcgtgcgc
    21001 gcgcacccgt cggcgtcggg cgaatcggtg gccatgctct atttcaccaa cagcctcggc
    21061 gcggcgttcg gggtgctggc gagcggcttc gtgctgatag cctgggtggg gctgccgggc
    21121 acgctgcgca tcgcgggcgt gatcaatctg gcgctcgccg gcatcgtctg gctgctgtca
    21181 cggccactgc gcgcgccggc gctcgaaacg cgccacgcgc tggaaaccgg cgtgctgacg
    21241 ttgctgctcg ccgcgagttt cgtgaccggc gtcgcctcgt tcatctacga gatcagctgg
    21301 atccgcatgc tctcgctggt gctcggcgcc tcgacgcact ctttcgagct gatgctctcg
    21361 accttcatcc tcggcctcgc gctcggcggc tacgcggtgc gccgccgcgt ggatcgcgtc
    21421 gcctcgcccg agcgacttct gggctgggtg caggtggtga tgggcctcgc cgcgctcgcc
    21481 acgctgccgg tgtacgaccg cagcttcgag ctgatggagc gcctcatggc cggcatcgcc
    21541 cgcaacgacg ccggctacgc cctgttcaac ctctccgggc aggcgatcgc ggcgctggtg
    21601 atgctgcccg ccacgttctg cgctggcatg acgttgccgc tcatcaccgg cgcgctgctg
    21661 cgacagggcg cgggcgaagg cgccatcggc cgcgtgtacg cagccaatac ggtaggcgcc
    21721 atcgccggcg tgctgttcgc gctgcacgta ggcctgcccg cgctcggcct gaagggggca
    21781 ctcatcgcag gcgctctgct cgatgccgcg ctcggccttt acctcctgta tcggttcgcc
    21841 gcgccgcgcg cgcactgggt gcccgtggcc gccgcctgcg cggcgctgtt cgccgccgcg
    21901 gcgctcgcct tcgagctcga tgccaacaag atgaccgcgg gcgtgtaccg gcacggcgac
    21961 ctcgacagct cgcgcgacgc gacggtgctc tacgcaaagg acggcaagac cgccaccgtg
    22021 cacctggtga agtacagctc ggccaccagc atccgcacca acggcaagtc cgacggctcg
    22081 atcaacctcg agggcgaggc ggaggccgac cgcggcaccg acgagatcac catggtgctc
    22141 accggcgcgc tgccgctcgc gctcaagccc gaggcgaaga gcgccgcggt gatcggcatc
    22201 ggcaccggcc tcaccacgca cacgctgctg caaagcctcg acctcgagcg cgtcgagacc
    22261 gtggagatcg aggcggcgat ggtgcaagct gcgcgcggct tcctgccgcg caacagcgcc
    22321 gcgttcgccg atccgcgcgg caccatcgtc atcgacgacg ccaagaccta cttttcggcg
    22381 cgccagcgac ggtacgacat catcgtctcg gagccctcga acccctgggt aagcggcgtc
    22441 tcgagcctgt ttacgcgcga gttctaccgt cgcatcgcca gccacctcgc ccctgggggt
    22501 ctgctggtgc agtggttcca gctctatgag atcgatccgg gcctggtcgc gtcggtactg
    22561 ggggctctgg gcgcggagtt cgcgcactac gcggtgtacg cgccgagcaa tcacgacgta
    22621 ctcatcgtgg cgagcgccgc gccgctgccg ccggcgccgc aggcgcgcgt gttcgagcac
    22681 ccgggcctgg cgaaggagct gtggaccgtg cacatgctct ccgccggcga tctcgacgcg
    22741 cgctacctgg gcggccgcgc cacgctcgag ccgctgttcg agagctacgc cgcgcgcgcc
    22801 aactcggatt actttccggt gctggacctg aacgcggtgc gccatcgctt cgccgagcgc
    22861 agcgccaccg gcatcgtggc gatcacgagc gccgcggttc ccgtgctcga tctgctcgag
    22921 ccgcagttcg tgcggcgccc gccgaacccg ctgcacaagg gcgcctatgc cttcgagcgc
    22981 atcgagaaga cgcgccaggc gcgctacgcg cgcgacttcc tggtgcatct ggcgccaccc
    23041 gtgcccgagg gcattccgcc gcagctgcaa aaggatctcg aggtggtgca gttgcgcctg
    23101 ctcggctgcc gcgaagcgcg gcgcttcgac gtctggctgc acagcctgct gcgcgtcgcc
    23161 gagctggtga acggggtgtt gccggcgggc gaagcggcgg cgctgtggtc gcgcatcggc
    23221 gcgacctcgt gcctaagcac gctgcacgac ttccagcgcc gctggatcgc gctgttcacg
    23281 gcagtgggcg cgcgcgatgc gcgcgccatg gcaagcctcg cctcggcgct gctcgagggc
    23341 acgggaaacc tgggcgcaga tgcgcgcgag tacctgctgc tggccggcat gagcggcgcg
    23401 atcgcctcgg ggcggcccga gcaggcgctc gcgctgtggc agtcgcaggc cccgctgctg
    23461 cgcggcgcgc tcgcgcagcc ggtgttccgg ctgctgcgct gccacgcgga gcgtgccggt
    23521 tgcgccgcag ctttcgtcgc ttacaacgag gactaggggg ccttgctgcg ccggggcgcc
    23581 ggtccacgcc cggcgtaggg attgcggccg gtgcggaatt ccacccgcat cggcgtgccg
    23641 cgcaaagcga acgtgtcgcg gaacaccccc tcgaggtagc gccggtagct ctcgggcaac
    23701 tggtcgagcg agttgccgtg gatgatgatg cgcggcggat tgtgcccgcc ttgatgcgca
    23761 tagcgcagct tggggcgccg caggcccttc ctcggcggcg cctgccgctc cacggcggca
    23821 atcagcgcgc gcgtgagctt gggcgtcggc atctttgcca tcgccgcggc gtatgcggcg
    23881 tccaccgagc gcatcagctc gcccaggccc ttgcccgcct tggccgagag gaagtgcgtc
    23941 tcggcgaagc ccaggaaccc cagcttccag gcgagctcgt gcttcacgcg ctcgcgcgct
    24001 tccttgcccg ccgcgtccca tttgttcacc gccacgacga gcgcgcgcgc acgctcgagg
    24061 atgtagccgg cgacatgcgc gtcctgctcc gacacgccct ccaccgcgtc cagcaccagg
    24121 atagccacgt tcgactgctc gatcgcctgc ggcgtcttta cgatggagaa cttctccacc
    24181 gtttcgccct gcctgccgcg cttgcgcagc cccgcggtgt cgatcagcgt gtagcgccgg
    24241 ccgccgcgct cgaacggcac ctcgatggcg tcgcgcgtgg tgccgggctc gccgaaggcg
    24301 atcacgcgct cctcgccgac cagcgcgttg accagggtcg acttgcccac gttcggccgt
    24361 ccgaccacgg cgacgcgtgg atgcttgtcg cgtgcttcct cgtcctcggc cgctgcctcc
    24421 ggaaacccgg cgagcacgcc ctcgatcagg tcggcgacgc cttcgccgtg cgcagcggaa
    24481 atcgcgttcg gctgccccag gccgagcgcg tggaactcgg ccaccgcgct gtcgctcgcc
    24541 atgccctcgc acttgttcgc caccagccag acgcgcgcgc cgatgcggcg cagacgcgca
    24601 gcgatctcgc ggtcgtcggc gtgcagcccg gcgcggccgt cggtgagaaa caccacggcg
    24661 tcggcctcgg ccatcgcctg ctcggcctgc cggcccatct cggcgaagat gccgctcttc
    24721 gcaccgggct cgaggccgcc cgtgtcgatg acgatgaacg ggcgcgcgcc cacgcgcccc
    24781 tcgccgtagt ggcggtcccg tgtcacgccc ggcacatcga tcacgatcgc gtcgcggcgg
    24841 cgcgtcaggc ggttgaatag cgtcgacttg ccgacgttgg gccgcccgac cagggcgatg
    24901 accggcttca cgcgcgcgcc cggctcaggg cgacagggcg aacagcccgc cgccctgcgt
    24961 ctgcaccaga aaaccacgcg acagcgcgag cggcgccgcg cgcaccgggc tgccatcggt
    25021 gcccaggcgc gcgacgaagg cgcccgagtc gcgcgccagc acgtgcacga acccttccag
    25081 gtcgccgagc acgacgctct cgcccaacgg cagcggcagc gacagctgcc gctgcgcgag
    25141 cttgtcctgc ttccacaggc tgcggccgtt ggtgcggtcg agcgcatgca ctgcaccccg
    25201 ctcgtcgctc agataggcat agcgcgcgtc cgcgctcacg ccggtcacgc tcgagatgtc
    25261 gcgcgcccac agcaggttgc cgttttgcgc gtcgaagcag gcaactcggc cctggaacgc
    25321 ggcggcgcat acctcgcgcc cgaccactgc cggctcgccg accacgtcgg tgacgcgctc
    25381 gagctcggtc gtgcccttgg ggtcggccac cgtcgcttcc catcgcagtg tgccgccggc
    25441 gagcgccagc gccaccagct tgccgcccga aaaccccgca tacagcaggt ccgcgtgcgg
    25501 agtcatgccc tggggcgtgc gcagccgcag cggcgcgggc gcgcgctggt aggtccagcg
    25561 ccgcttgccg tcggccgcat ccagcgcgtg tatgcggctg tcggcgctgc gcaccagcac
    25621 cattccggcg gccagccgcg gcgccgcaag cacctcgctc gagacgcgcg cgcgccagcg
    25681 ctccttgccg gtgagcgcat cgagcgcgaa caccgcgccg ctctccgtcg ccacggcgag
    25741 cgtcgtgcca tccgccccga cgccgcccga cagcggcgct tccacgcgca cgcgccagcg
    25801 ctcgcggccg ctggcggcat ccaggcgcag caccgtgccg tcgcgcgccg cggcgtagag
    25861 cgcatcgccg gcgagcgcag gactgaattc gaacgcggcc gccgcgccca cgtttgccga
    25921 ccacagcacg cgcagtgcct gcggctgcgc cagcggctcg agcgccgcgg gctgcgggcc
    25981 gtcagacgag ccgccgaagc agccgctgag cgcgggcagc agcagggcga gcgccgcgcg
    26041 ccgcgccatg ctcagcctcc gagcgcgtct aggcgcatct gtacgctgtc gcgcaacggc
    26101 ccgcccgcct tctccagcgc cagctggtag gcggcgcgcg cctccgccgg ctggcccttt
    26161 gcaaccagca catcgccgcg cagcgccgca aactgcggcg cgtatggctc gagcggcggc
    26221 gccgcaagca gcgcgagcgc ctcgtcgtag gcgcgctcgt cgagcagcag cgcggcgagg
    26281 cgcagtcgcg cgacgtcgcg gaagtcctcg gacgaagagc ggtcgactac ccactgcagc
    26341 tgcgccttgg cgctcttcag ctcgccgcgc ttgaagtgga aatccgccgc agcgagcgcg
    26401 cccatcgacg cgtacagcgt cccggcgaaa cgctcggtga gcgcgccgcc cgcgtcgcgc
    26461 agcgccttgg cgtccccggc gcgcaccgcc ttggcgagcg tggcgtagag cgcgcccgcc
    26521 tcgagcgcct gctggcgctg gtaccagcgc cagccgttgg tgccggtgac ggcgatcgcc
    26581 gccgcagcca ccaccagcac gatgaggcgc gcattgtcct tccaccacgc cttgagcgcg
    26641 tcgagctttt cctgctcttc caggtcgagg ttctgcagtg ccatggtcag gatcccttga
    26701 gaagttcgtg caggcgcgac gccagcgcgg cgcgcgccac ctgctgctgg ggccgcgcgt
    26761 cgcgcagcgg cttcacggtt accgcaccgg ccttgagctc gtcttcgccg acgatcagcg
    26821 ccagcgacgc gccgctcgca tcggccttct tcatctgcga cttgaagctg ccgccgcccg
    26881 catgcagcac gaccgcgaaa cccgccgccc gcaattgttc accgagcgcg aacgcatacg
    26941 ccagcgtgcc ctcgccctgg tgcaccaggt aggcgccggg cgccacaggc gccggcgcgc
    27001 cgagcagcgc gaccagccgc tcgatcccgg ccgcgaagcc cacgccgggg gcgggcttgc
    27061 cgccgagctg cgcgaacagg ccatcgtagc gcccgccacc acataccgtg ccctgggcgc
    27121 ccagccggtc gctcacccac tcgaacaccg tgagattgta gtagtccagg ccgcgcacca
    27181 gcttcgtgtt caccgtgaac gccacgcccg ccgtggcgag cagccccttg aggccctcga
    27241 aatgcgccag cgaagtctcg cccaggtaat ccatgagcgc cggtgcgctc gccacgacct
    27301 gctgcaccat cgggttcttc gaatccagca cgcgcaaggg attcgtgtgc agccggcgct
    27361 tcgcgtcggc gtcgagcgcc gacgcgtggg cttcgagatg ccggatcagg tcggtacggt
    27421 agcgcccgcg ctcttcgctc gagccgatgg tgttcagctc gagccggatg ccgtccaggc
    27481 cgagctcccg ccacagcgcc gcgcacatca cgatcagctc ggcgtcgaca tcgggcccgg
    27541 cgaagccgag cgcctcggcg ccgaactggt ggaattcgcg gtagcggccc ttttgcggcc
    27601 gctcgtggcg gtacatcggc ccctggtacc aaagccgctt ggggccttcg tagagcaggc
    27661 tgtgctcgat cgccgcacgc accgtagagg cggtggcttc gggccgcagc gtcaggctct
    27721 cgtcgttgag cgcgtcggtg aaggtgtaca tctccttctc gacgatgtcg gtcgcctcgc
    27781 cgatggcgcg gcgaaacagc gccgtgtgct cgagcagcgg cgtgcgcagg tgccggtagc
    27841 cgtagcgcgc gaaccagcgc gccacgacgc cctcgagcca caggcgctgc tcggcggcat
    27901 cgggcaatac gtcgttcatg ccgcgaacgg cctgcagagg cgtgctcacg gctcaggcga
    27961 ccttgatcgg aatgctgcgg tcacgggggc gggaggcgcc cggcgcataa ttcctgcgca
    28021 cgtagtcctc gacgatcgcc tggaactcct cggcgatgcg cgcgcccttc agggtgacgg
    28081 tcttcgcgcc gtccacgtac accggcgcca ccggcgtctc gttggtcccc ggcaggctga
    28141 tgccgatgtc ggcatgcttg gattcgccgg gcccgttcac cacgcaaccc atcaccgcga
    28201 cgcgcatctt ctcaacgccg ggatagcgtg cacgccactg cggcatctgc tcgcgcagga
    28261 aggcctggat gcgcgcggcg agctcctgaa aataggtgct ggtggttcgc ccgcaccccg
    28321 gacaggcggc gaccatcggc gcgaacgagc ggatgcccat ggtctgcagc agctcctgcg
    28381 ccaccagcac ctcgcgcgtg cgctccccgc cggggtccgg cgtgagcgag atccggatcg
    28441 tgtcgccgat gccctcctgc agcagcaccg cgagcgccgc gctggaagcg acgatgccct
    28501 tcgagcccat gcccgcctcc gtcaggccga gatgcagcgc gtaggtgcag cgccgcgcga
    28561 gctcgcggta caccgcgatc agatcctgca cctggctcat cttgcaggag atgacgatgc
    28621 gctcgggccc gaggccgagc tcttcggcgg cgcgcgccga ctcgagcgcg gaagcgatca
    28681 ccgcctcgcg catcacggcg tcggcctcga gcgggtcgcg gcgccgcgca ttctcgtcca
    28741 tcatgcgcgc gagcagctcg gcgtcgaggc tgccccagtt gacgccgatg cgcacgggct
    28801 tcgcgtagcg caccgccgcc tcgatcatgg tcgcgaactg ctcgtcgcgc tttgcgccgc
    28861 ggccgacgtt gccgggattg atacggtact tggccagcgc gcgcgcgcaa tcgggaaact
    28921 gcgccagcag cttgtggccg ttgtagtgga aatcgcccac cagcggcacg gcgcagccca
    28981 gcgcgtcgag ccgcgcccga atcggcgcca cctgcgccgc ggcctccggc gtgttcaccg
    29041 tgatgcgtac gagctcggag ccggcggccg cgagctcggc cacctggcgc gcagtggcct
    29101 cggcgtcggc ggtatcggtg ttggtcatcg actgcacgac cacgggcgcg ccgccgccga
    29161 tggcgacggg gcccaccatc acgcggctgg agaacctgcg ctcgagcatg tcagtccagc
    29221 gtcaggcgcg cgacttcgac cttggtgtgc ggcttcaggt cgaccggctg gtcgttgtag
    29281 acgagctgca cgtgctgcgc gttgccgatc acgacgctga acggcggcac cccgaccacc
    29341 acgcgctcgc tgccctcggc atgcagctgt gagacgagga gcttgccggt gccgctggtg
    29401 atctcgaccc acgactcgcg ctcgaagcgc agcacgatgc gccgccaggc gccgctcgca
    29461 atgcgcgctt cctcgtccac gggcgcggcg gcgggctgcg ccgcggcgct ggcgcggggt
    29521 gcggcgctcg ccagctcggt gcgcgccggc gtttcctggc gcgcggg
//
